LOCUS       DAZGSP010000001       135899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZGSP010000001 DAZGSP010000000
VERSION     DAZGSP010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 135899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 135899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..135899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.001"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..416)
                     /locus_tag="ACHK74_000001"
     CDS             complement(<1..416)
                     /locus_tag="ACHK74_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP0218890.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     VGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYR"
     gene            424..930
                     /gene="tnpA"
                     /locus_tag="ACHK74_000002"
                     /pseudo
     CDS             424..930
                     /gene="tnpA"
                     /locus_tag="ACHK74_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(948..1616)
                     /gene="ydcL"
                     /locus_tag="ACHK74_000003"
     CDS             complement(948..1616)
                     /gene="ydcL"
                     /locus_tag="ACHK74_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFP0218891.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPIALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(1918..2511)
                     /gene="tehB"
                     /locus_tag="ACHK74_000004"
     CDS             complement(1918..2511)
                     /gene="tehB"
                     /locus_tag="ACHK74_000004"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFP0218892.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(2508..3500)
                     /gene="tehA"
                     /locus_tag="ACHK74_000005"
     CDS             complement(2508..3500)
                     /gene="tehA"
                     /locus_tag="ACHK74_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFP0218893.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVMSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            3624..4604
                     /gene="ydcK"
                     /locus_tag="ACHK74_000006"
     CDS             3624..4604
                     /gene="ydcK"
                     /locus_tag="ACHK74_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFP0218894.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(4599..5135)
                     /gene="rimL"
                     /locus_tag="ACHK74_000007"
     CDS             complement(4599..5135)
                     /gene="rimL"
                     /locus_tag="ACHK74_000007"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFP0218895.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLSKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(5198..5422)
                     /gene="ydcH"
                     /locus_tag="ACHK74_000008"
     CDS             complement(5198..5422)
                     /gene="ydcH"
                     /locus_tag="ACHK74_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFP0218896.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(5562..7217)
                     /gene="opgD"
                     /locus_tag="ACHK74_000009"
                     /gene_synonym="mdoD"
     CDS             complement(5562..7217)
                     /gene="opgD"
                     /locus_tag="ACHK74_000009"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFP0218897.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(7442..8785)
                     /gene="ydcJ"
                     /locus_tag="ACHK74_000010"
     CDS             complement(7442..8785)
                     /gene="ydcJ"
                     /locus_tag="ACHK74_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP0218898.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            9002..9925
                     /gene="ydcI"
                     /locus_tag="ACHK74_000011"
     CDS             9002..9925
                     /gene="ydcI"
                     /locus_tag="ACHK74_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0218899.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEDLNNDV
                     VRVGALPTAALGILPLVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(9963..11603)
                     /gene="trg"
                     /locus_tag="ACHK74_000012"
     CDS             complement(9963..11603)
                     /gene="trg"
                     /locus_tag="ACHK74_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFP0218900.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            12002..12151
                     /gene="hokB"
                     /locus_tag="ACHK74_000013"
     CDS             12002..12151
                     /gene="hokB"
                     /locus_tag="ACHK74_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFP0218901.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(12129..12197)
                     /gene="yncP"
                     /locus_tag="ACHK74_000014"
     CDS             complement(12129..12197)
                     /gene="yncP"
                     /locus_tag="ACHK74_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFP0218902.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(12223..12396)
                     /locus_tag="ACHK74_000015"
     CDS             complement(12223..12396)
                     /locus_tag="ACHK74_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0218903.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(12641..13171)
                     /gene="cybB"
                     /locus_tag="ACHK74_000016"
     CDS             complement(12641..13171)
                     /gene="cybB"
                     /locus_tag="ACHK74_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFP0218904.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            13360..14361
                     /gene="gap"
                     /locus_tag="ACHK74_000017"
     CDS             13360..14361
                     /gene="gap"
                     /locus_tag="ACHK74_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP0218905.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(14403..15842)
                     /gene="aldA"
                     /locus_tag="ACHK74_000018"
     CDS             complement(14403..15842)
                     /gene="aldA"
                     /locus_tag="ACHK74_000018"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFP0218906.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVDGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(16039..16839)
                     /gene="ydcF"
                     /locus_tag="ACHK74_000019"
     CDS             complement(16039..16839)
                     /gene="ydcF"
                     /locus_tag="ACHK74_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFP0218907.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(17111..21013)
                     /gene="hrpA"
                     /locus_tag="ACHK74_000020"
     CDS             complement(17111..21013)
                     /gene="hrpA"
                     /locus_tag="ACHK74_000020"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFP0218908.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARRKDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            21214..21819
                     /gene="azoR"
                     /locus_tag="ACHK74_000021"
     CDS             21214..21819
                     /gene="azoR"
                     /locus_tag="ACHK74_000021"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFP0218909.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVNFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(21870..23186)
                     /gene="ynbD"
                     /locus_tag="ACHK74_000022"
     CDS             complement(21870..23186)
                     /gene="ynbD"
                     /locus_tag="ACHK74_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFP0218910.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVNEAISYIRARRPQIVLKEDHKAMLKLWENR"
     gene            complement(23176..24933)
                     /gene="ynbC"
                     /locus_tag="ACHK74_000023"
     CDS             complement(23176..24933)
                     /gene="ynbC"
                     /locus_tag="ACHK74_000023"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFP0218911.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYMPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGSVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSIGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(24949..25845)
                     /gene="ynbB"
                     /locus_tag="ACHK74_000024"
     CDS             complement(24949..25845)
                     /gene="ynbB"
                     /locus_tag="ACHK74_000024"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP0218912.1"
                     /translation="MLEKSLVTLFALLILATLINHFLLWRLPERKGGEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISIHFPRWLYWVIPLNYLLIGFNC
                     FELFLLFIPLSGFLILATWRVFVGEPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPS
                     MNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(25845..26450)
                     /gene="ynbA"
                     /locus_tag="ACHK74_000025"
     CDS             complement(25845..26450)
                     /gene="ynbA"
                     /locus_tag="ACHK74_000025"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP0218913.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMVLNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLFMSAER"
     gene            complement(26621..28927)
                     /gene="ydbD"
                     /locus_tag="ACHK74_000026"
     CDS             complement(26621..28927)
                     /gene="ydbD"
                     /locus_tag="ACHK74_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFP0218914.1"
                     /translation="MFKARICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIIR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(28991..29851)
                     /gene="pdxI"
                     /locus_tag="ACHK74_000027"
     CDS             complement(28991..29851)
                     /gene="pdxI"
                     /locus_tag="ACHK74_000027"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFP0218915.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRIMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(30083..30673)
                     /gene="paaY"
                     /locus_tag="ACHK74_000028"
     CDS             complement(30083..30673)
                     /gene="paaY"
                     /locus_tag="ACHK74_000028"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFP0218916.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPGRKRLVFDENLRPKQ"
     gene            complement(30655..31605)
                     /gene="paaX"
                     /locus_tag="ACHK74_000029"
     CDS             complement(30655..31605)
                     /gene="paaX"
                     /locus_tag="ACHK74_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFP0218917.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(31706..33019)
                     /gene="paaK"
                     /locus_tag="ACHK74_000030"
     CDS             complement(31706..33019)
                     /gene="paaK"
                     /locus_tag="ACHK74_000030"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HFP0218918.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(33046..34251)
                     /gene="paaJ"
                     /locus_tag="ACHK74_000031"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(33046..34251)
                     /gene="paaJ"
                     /locus_tag="ACHK74_000031"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFP0218919.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(34251..34673)
                     /gene="paaI"
                     /locus_tag="ACHK74_000032"
     CDS             complement(34251..34673)
                     /gene="paaI"
                     /locus_tag="ACHK74_000032"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFP0218920.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(34663..36090)
                     /gene="paaH"
                     /locus_tag="ACHK74_000033"
     CDS             complement(34663..36090)
                     /gene="paaH"
                     /locus_tag="ACHK74_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFP0218921.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(36092..36880)
                     /gene="paaG"
                     /locus_tag="ACHK74_000034"
     CDS             complement(36092..36880)
                     /gene="paaG"
                     /locus_tag="ACHK74_000034"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFP0218922.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALECDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(36880..37647)
                     /gene="paaF"
                     /locus_tag="ACHK74_000035"
     CDS             complement(36880..37647)
                     /gene="paaF"
                     /locus_tag="ACHK74_000035"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFP0218923.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(37644..38714)
                     /gene="paaE"
                     /locus_tag="ACHK74_000036"
     CDS             complement(37644..38714)
                     /gene="paaE"
                     /locus_tag="ACHK74_000036"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFP0218924.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(38722..39219)
                     /gene="paaD"
                     /locus_tag="ACHK74_000037"
     CDS             complement(38722..39219)
                     /gene="paaD"
                     /locus_tag="ACHK74_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFP0218925.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(39234..39980)
                     /gene="paaC"
                     /locus_tag="ACHK74_000038"
     CDS             complement(39234..39980)
                     /gene="paaC"
                     /locus_tag="ACHK74_000038"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFP0218926.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(39989..40276)
                     /gene="paaB"
                     /locus_tag="ACHK74_000039"
     CDS             complement(39989..40276)
                     /gene="paaB"
                     /locus_tag="ACHK74_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFP0218927.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(40288..41217)
                     /gene="paaA"
                     /locus_tag="ACHK74_000040"
     CDS             complement(40288..41217)
                     /gene="paaA"
                     /locus_tag="ACHK74_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFP0218928.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQHARKVA"
     gene            41502..43547
                     /gene="paaZ"
                     /locus_tag="ACHK74_000041"
     CDS             41502..43547
                     /gene="paaZ"
                     /locus_tag="ACHK74_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFP0218929.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQRHALQLACAGGGSLAGTLVTADPQIARQFITDAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            43795..46068
                     /gene="tynA"
                     /locus_tag="ACHK74_000042"
     CDS             43795..46068
                     /gene="tynA"
                     /locus_tag="ACHK74_000042"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFP0218930.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDETL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(46125..47624)
                     /gene="feaB"
                     /locus_tag="ACHK74_000043"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(46125..47624)
                     /gene="feaB"
                     /locus_tag="ACHK74_000043"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HFP0218931.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            47860..48765
                     /gene="feaR"
                     /locus_tag="ACHK74_000044"
     CDS             47860..48765
                     /gene="feaR"
                     /locus_tag="ACHK74_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFP0218932.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGHLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(48937..49266)
                     /gene="ydbL"
                     /locus_tag="ACHK74_000045"
     CDS             complement(48937..49266)
                     /gene="ydbL"
                     /locus_tag="ACHK74_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFP0218933.1"
                     /translation="MMKKTLLFCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(49271..49456)
                     /gene="ynbE"
                     /locus_tag="ACHK74_000046"
     CDS             complement(49271..49456)
                     /gene="ynbE"
                     /locus_tag="ACHK74_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFP0218934.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(49453..52092)
                     /gene="ydbH"
                     /locus_tag="ACHK74_000047"
     CDS             complement(49453..52092)
                     /gene="ydbH"
                     /locus_tag="ACHK74_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFP0218935.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMEKSQMPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYYHEENIFTLWRSLRFGDNLQA
                     WLEQNARLLGNDCPQGKECEEKQ"
     gene            52300..53289
                     /gene="ldhA"
                     /locus_tag="ACHK74_000048"
     CDS             52300..53289
                     /gene="ldhA"
                     /locus_tag="ACHK74_000048"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP0218936.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            53400..53822
                     /gene="hslJ"
                     /locus_tag="ACHK74_000049"
     CDS             53400..53822
                     /gene="hslJ"
                     /locus_tag="ACHK74_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFP0218937.1"
                     /translation="MKKVAALVALSLLMAGCVSSDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(53819..54085)
                     /gene="ydbJ"
                     /locus_tag="ACHK74_000050"
     CDS             complement(53819..54085)
                     /gene="ydbJ"
                     /locus_tag="ACHK74_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFP0218938.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            54359..57883
                     /gene="nifJ"
                     /locus_tag="ACHK74_000051"
     CDS             54359..57883
                     /gene="nifJ"
                     /locus_tag="ACHK74_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFP0218939.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQVEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            58250..59383
                     /gene="ompN"
                     /locus_tag="ACHK74_000052"
     CDS             58250..59383
                     /gene="ompN"
                     /locus_tag="ACHK74_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFP0218940.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            59524..59958
                     /gene="uspF"
                     /locus_tag="ACHK74_000053"
     CDS             59524..59958
                     /gene="uspF"
                     /locus_tag="ACHK74_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFP0218941.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            60134..61069
                     /gene="ttcA"
                     /locus_tag="ACHK74_000054"
     CDS             60134..61069
                     /gene="ttcA"
                     /locus_tag="ACHK74_000054"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFP0218942.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(61198..62571)
                     /gene="dbpA"
                     /locus_tag="ACHK74_000055"
     CDS             complement(61198..62571)
                     /gene="dbpA"
                     /locus_tag="ACHK74_000055"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFP0218943.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQVAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(62601..62774)
                     /gene="ynaL"
                     /locus_tag="ACHK74_000056"
     CDS             complement(62601..62774)
                     /gene="ynaL"
                     /locus_tag="ACHK74_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFP0218944.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(63049..64032)
                     /gene="zntB"
                     /locus_tag="ACHK74_000057"
     CDS             complement(63049..64032)
                     /gene="zntB"
                     /locus_tag="ACHK74_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFP0218945.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHESAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(64046..64093)
                     /gene="ydaN"
                     /locus_tag="ACHK74_000058"
     CDS             complement(64046..64093)
                     /gene="ydaN"
                     /locus_tag="ACHK74_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFP0218946.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            64287..65519
                     /gene="dgcM"
                     /locus_tag="ACHK74_000059"
     CDS             64287..65519
                     /gene="dgcM"
                     /locus_tag="ACHK74_000059"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFP0218947.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKLLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(65540..66103)
                     /gene="smrA"
                     /locus_tag="ACHK74_000060"
     CDS             complement(65540..66103)
                     /gene="smrA"
                     /locus_tag="ACHK74_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFP0218948.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(66433..67341)
                     /gene="abgR"
                     /locus_tag="ACHK74_000061"
     CDS             complement(66433..67341)
                     /gene="abgR"
                     /locus_tag="ACHK74_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0218949.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            67517..68827
                     /gene="abgA"
                     /locus_tag="ACHK74_000062"
     CDS             67517..68827
                     /gene="abgA"
                     /locus_tag="ACHK74_000062"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFP0218950.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            68827..70272
                     /gene="abgB"
                     /locus_tag="ACHK74_000063"
     CDS             68827..70272
                     /gene="abgB"
                     /locus_tag="ACHK74_000063"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFP0218951.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            70306..71832
                     /gene="abgT"
                     /locus_tag="ACHK74_000064"
     CDS             70306..71832
                     /gene="abgT"
                     /locus_tag="ACHK74_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFP0218952.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            71843..72358
                     /gene="ogt"
                     /locus_tag="ACHK74_000065"
     CDS             71843..72358
                     /gene="ogt"
                     /locus_tag="ACHK74_000065"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFP0218953.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            72553..73305
                     /gene="fnr"
                     /locus_tag="ACHK74_000066"
     CDS             72553..73305
                     /gene="fnr"
                     /locus_tag="ACHK74_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFP0218954.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            73457..74407
                     /gene="uspE"
                     /locus_tag="ACHK74_000067"
     CDS             73457..74407
                     /gene="uspE"
                     /locus_tag="ACHK74_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFP0218955.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(74457..74714)
                     /gene="ynaJ"
                     /locus_tag="ACHK74_000068"
     CDS             complement(74457..74714)
                     /gene="ynaJ"
                     /locus_tag="ACHK74_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFP0218956.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            74958..75989
                     /gene="ynaI"
                     /locus_tag="ACHK74_000069"
     CDS             74958..75989
                     /gene="ynaI"
                     /locus_tag="ACHK74_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFP0218957.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPQEQGR"
     gene            complement(76040..77653)
                     /gene="mppA"
                     /locus_tag="ACHK74_000070"
     CDS             complement(76040..77653)
                     /gene="mppA"
                     /locus_tag="ACHK74_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFP0218958.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(77990..78889)
                     /gene="pgrR"
                     /locus_tag="ACHK74_000071"
     CDS             complement(77990..78889)
                     /gene="pgrR"
                     /locus_tag="ACHK74_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFP0218959.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSAMASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            79027..79947
                     /locus_tag="ACHK74_000072"
     CDS             79027..79947
                     /locus_tag="ACHK74_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0218960.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVLSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            79947..80879
                     /locus_tag="ACHK74_000073"
     CDS             79947..80879
                     /locus_tag="ACHK74_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0218961.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            80897..81535
                     /locus_tag="ACHK74_000074"
     CDS             80897..81535
                     /locus_tag="ACHK74_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0218962.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGDNIGINQPVTDGDKPLFM"
     gene            81844..82572
                     /gene="mpaA"
                     /locus_tag="ACHK74_000075"
     CDS             81844..82572
                     /gene="mpaA"
                     /locus_tag="ACHK74_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFP0218963.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(82547..83512)
                     /gene="ycjG"
                     /locus_tag="ACHK74_000076"
     CDS             complement(82547..83512)
                     /gene="ycjG"
                     /locus_tag="ACHK74_000076"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFP0218964.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            83631..84137
                     /gene="tpx"
                     /locus_tag="ACHK74_000077"
     CDS             83631..84137
                     /gene="tpx"
                     /locus_tag="ACHK74_000077"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFP0218965.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(84181..85722)
                     /gene="tyrR"
                     /locus_tag="ACHK74_000078"
     CDS             complement(84181..85722)
                     /gene="tyrR"
                     /locus_tag="ACHK74_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFP0218966.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(85870..86931)
                     /gene="ycjF"
                     /locus_tag="ACHK74_000079"
     CDS             complement(85870..86931)
                     /gene="ycjF"
                     /locus_tag="ACHK74_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFP0218967.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(86928..88325)
                     /gene="ycjX"
                     /locus_tag="ACHK74_000080"
     CDS             complement(86928..88325)
                     /gene="ycjX"
                     /locus_tag="ACHK74_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFP0218968.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            88480..89478
                     /gene="ycjW"
                     /locus_tag="ACHK74_000081"
     CDS             88480..89478
                     /gene="ycjW"
                     /locus_tag="ACHK74_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0218969.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(89589..90494)
                     /gene="ompG"
                     /locus_tag="ACHK74_000082"
     CDS             complement(89589..90494)
                     /gene="ompG"
                     /locus_tag="ACHK74_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFP0218970.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(90539..91621)
                     /locus_tag="ACHK74_000083"
     CDS             complement(90539..91621)
                     /locus_tag="ACHK74_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0218971.1"
                     /translation="MAQLSLQHIQEIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(91635..92294)
                     /gene="pgmB"
                     /locus_tag="ACHK74_000084"
     CDS             complement(91635..92294)
                     /gene="pgmB"
                     /locus_tag="ACHK74_000084"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP0218972.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFLAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(92291..94558)
                     /gene="ycjT"
                     /locus_tag="ACHK74_000085"
     CDS             complement(92291..94558)
                     /gene="ycjT"
                     /locus_tag="ACHK74_000085"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFP0218973.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSHEAWQRELDFASGELR
                     RNVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQKCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTIARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     LFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWCEGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEGE"
     gene            complement(94555..95610)
                     /gene="ycjS"
                     /locus_tag="ACHK74_000086"
     CDS             complement(94555..95610)
                     /gene="ycjS"
                     /locus_tag="ACHK74_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP0218974.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFTLDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(95620..96396)
                     /gene="ycjR"
                     /locus_tag="ACHK74_000087"
     CDS             complement(95620..96396)
                     /gene="ycjR"
                     /locus_tag="ACHK74_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP0218975.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVRVLEQVAARTGTVVYLEPLNRYQDHMINTLADARR
                     YIVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGTLDFH
                     ALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(96415..97467)
                     /gene="ycjQ"
                     /locus_tag="ACHK74_000088"
     CDS             complement(96415..97467)
                     /gene="ycjQ"
                     /locus_tag="ACHK74_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFP0218976.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(97498..98340)
                     /gene="ycjP"
                     /locus_tag="ACHK74_000089"
     CDS             complement(97498..98340)
                     /gene="ycjP"
                     /locus_tag="ACHK74_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP0218977.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(98327..99208)
                     /gene="ycjO"
                     /locus_tag="ACHK74_000090"
     CDS             complement(98327..99208)
                     /gene="ycjO"
                     /locus_tag="ACHK74_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP0218978.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVSLSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(99229..100521)
                     /gene="ycjN"
                     /locus_tag="ACHK74_000091"
     CDS             complement(99229..100521)
                     /gene="ycjN"
                     /locus_tag="ACHK74_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP0218979.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(100535..102214)
                     /gene="ycjM"
                     /locus_tag="ACHK74_000092"
     CDS             complement(100535..102214)
                     /gene="ycjM"
                     /locus_tag="ACHK74_000092"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFP0218980.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     NESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(102426..102740)
                     /gene="pspE"
                     /locus_tag="ACHK74_000093"
     CDS             complement(102426..102740)
                     /gene="pspE"
                     /locus_tag="ACHK74_000093"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFP0218981.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(102815..103036)
                     /gene="pspD"
                     /locus_tag="ACHK74_000094"
     CDS             complement(102815..103036)
                     /gene="pspD"
                     /locus_tag="ACHK74_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFP0218982.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(103045..103404)
                     /gene="pspC"
                     /locus_tag="ACHK74_000095"
     CDS             complement(103045..103404)
                     /gene="pspC"
                     /locus_tag="ACHK74_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFP0218983.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPAKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(103404..103628)
                     /gene="pspB"
                     /locus_tag="ACHK74_000096"
     CDS             complement(103404..103628)
                     /gene="pspB"
                     /locus_tag="ACHK74_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFP0218984.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(103682..104350)
                     /gene="pspA"
                     /locus_tag="ACHK74_000097"
     CDS             complement(103682..104350)
                     /gene="pspA"
                     /locus_tag="ACHK74_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFP0218985.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            104517..105494
                     /gene="pspF"
                     /locus_tag="ACHK74_000098"
     CDS             104517..105494
                     /gene="pspF"
                     /locus_tag="ACHK74_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFP0218986.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(105614..106879)
                     /gene="puuE"
                     /locus_tag="ACHK74_000099"
     CDS             complement(105614..106879)
                     /gene="puuE"
                     /locus_tag="ACHK74_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFP0218987.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGKPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDVQFDAAMKILQDALSD"
     gene            complement(106917..108197)
                     /gene="puuB"
                     /locus_tag="ACHK74_000100"
     CDS             complement(106917..108197)
                     /gene="puuB"
                     /locus_tag="ACHK74_000100"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFP0218988.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHNTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(108199..109686)
                     /gene="puuC"
                     /locus_tag="ACHK74_000101"
     CDS             complement(108199..109686)
                     /gene="puuC"
                     /locus_tag="ACHK74_000101"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFP0218989.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAMSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGESNMKRVWLEAGGKSANIV
                     FADCSDLQKAASSTAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(109961..110518)
                     /gene="puuR"
                     /locus_tag="ACHK74_000102"
     CDS             complement(109961..110518)
                     /gene="puuR"
                     /locus_tag="ACHK74_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFP0218990.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(110545..111309)
                     /gene="puuD"
                     /locus_tag="ACHK74_000103"
     CDS             complement(110545..111309)
                     /gene="puuD"
                     /locus_tag="ACHK74_000103"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFP0218991.1"
                     /translation="MENIMNNPVIGVLMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            111521..112939
                     /gene="puuA"
                     /locus_tag="ACHK74_000104"
     CDS             111521..112939
                     /gene="puuA"
                     /locus_tag="ACHK74_000104"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFP0218992.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            113088..113252
                     /locus_tag="ACHK74_000105"
     CDS             113088..113252
                     /locus_tag="ACHK74_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0218993.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRLTRPQTL
                     LRRGGSNYGY"
     gene            113242..114630
                     /gene="puuP"
                     /locus_tag="ACHK74_000106"
     CDS             113242..114630
                     /gene="puuP"
                     /locus_tag="ACHK74_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFP0218994.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGTYLWYLIRRYRKVPL
                     YEGDRTPVSETL"
     gene            114761..115006
                     /gene="ymjA"
                     /locus_tag="ACHK74_000107"
     CDS             114761..115006
                     /gene="ymjA"
                     /locus_tag="ACHK74_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFP0218995.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            115319..116962
                     /gene="sapA"
                     /locus_tag="ACHK74_000108"
     CDS             115319..116962
                     /gene="sapA"
                     /locus_tag="ACHK74_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFP0218996.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            116959..117924
                     /gene="sapB"
                     /locus_tag="ACHK74_000109"
     CDS             116959..117924
                     /gene="sapB"
                     /locus_tag="ACHK74_000109"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFP0218997.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            117911..118801
                     /gene="sapC"
                     /locus_tag="ACHK74_000110"
     CDS             117911..118801
                     /gene="sapC"
                     /locus_tag="ACHK74_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFP0218998.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            118801..119793
                     /gene="sapD"
                     /locus_tag="ACHK74_000111"
     CDS             118801..119793
                     /gene="sapD"
                     /locus_tag="ACHK74_000111"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFP0218999.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            119795..120601
                     /gene="sapF"
                     /locus_tag="ACHK74_000112"
     CDS             119795..120601
                     /gene="sapF"
                     /locus_tag="ACHK74_000112"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFP0219000.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            120669..121022
                     /gene="ycjD"
                     /locus_tag="ACHK74_000113"
     CDS             120669..121022
                     /gene="ycjD"
                     /locus_tag="ACHK74_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFP0219001.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQSWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            121390..122178
                     /gene="fabI"
                     /locus_tag="ACHK74_000114"
     CDS             121390..122178
                     /gene="fabI"
                     /locus_tag="ACHK74_000114"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFP0219002.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            122323..123450
                     /gene="yciW"
                     /locus_tag="ACHK74_000115"
     CDS             122323..123450
                     /gene="yciW"
                     /locus_tag="ACHK74_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFP0219003.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISALVRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            123518..125452
                     /gene="rnb"
                     /locus_tag="ACHK74_000116"
     CDS             123518..125452
                     /gene="rnb"
                     /locus_tag="ACHK74_000116"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFP0219004.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(125530..125634)
                     /locus_tag="ACHK74_000117"
     CDS             complement(125530..125634)
                     /locus_tag="ACHK74_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219005.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            125688..127673
                     /gene="pdeR"
                     /locus_tag="ACHK74_000118"
                     /pseudo
     CDS             125688..127673
                     /gene="pdeR"
                     /locus_tag="ACHK74_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(127655..127783)
                     /gene="ymiD"
                     /locus_tag="ACHK74_000119"
     CDS             complement(127655..127783)
                     /gene="ymiD"
                     /locus_tag="ACHK74_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFP0219006.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            127820..127993
                     /gene="yciZ"
                     /locus_tag="ACHK74_000120"
     CDS             127820..127993
                     /gene="yciZ"
                     /locus_tag="ACHK74_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFP0219007.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            128083..128832
                     /gene="yciT"
                     /locus_tag="ACHK74_000121"
     CDS             128083..128832
                     /gene="yciT"
                     /locus_tag="ACHK74_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFP0219008.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            129101..129319
                     /gene="osmB"
                     /locus_tag="ACHK74_000122"
     CDS             129101..129319
                     /gene="osmB"
                     /locus_tag="ACHK74_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFP0219009.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(129445..129771)
                     /gene="yciH"
                     /locus_tag="ACHK74_000123"
     CDS             complement(129445..129771)
                     /gene="yciH"
                     /locus_tag="ACHK74_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFP0219010.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(129771..130508)
                     /gene="pyrF"
                     /locus_tag="ACHK74_000124"
     CDS             complement(129771..130508)
                     /gene="pyrF"
                     /locus_tag="ACHK74_000124"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFP0219011.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(130701..131870)
                     /gene="lapB"
                     /locus_tag="ACHK74_000125"
     CDS             complement(130701..131870)
                     /gene="lapB"
                     /locus_tag="ACHK74_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFP0219012.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(131877..132185)
                     /gene="lapA"
                     /locus_tag="ACHK74_000126"
     CDS             complement(131877..132185)
                     /gene="lapA"
                     /locus_tag="ACHK74_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFP0219013.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(132334..133098)
                     /gene="pgpB"
                     /locus_tag="ACHK74_000127"
     CDS             complement(132334..133098)
                     /gene="pgpB"
                     /locus_tag="ACHK74_000127"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFP0219014.1"
                     /translation="MRSIARRTAVGVALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            133268..133858
                     /gene="ribA"
                     /locus_tag="ACHK74_000128"
     CDS             133268..133858
                     /gene="ribA"
                     /locus_tag="ACHK74_000128"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFP0219015.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(133922..>135899)
                     /gene="acnA"
                     /locus_tag="ACHK74_000129"
     CDS             complement(133922..>135899)
                     /gene="acnA"
                     /locus_tag="ACHK74_000129"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFP0219016.1"
                     /translation="EAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRK
                     HGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSED
                     QVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAF
                     AASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLA
                     KKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSG
                     PLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNIN
                     LASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINV
                     TRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSI
                     KPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRH
                     LPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFE
                     RIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGS
                     QEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT        33668 a        35448 c        33004 g        33779 t
ORIGIN      
        1 ttccggtagc gcatccagcc cagtttcggc agaaaaatac ggctgttttc ctgatcgagc
       61 ttaacgccct gcgggtagcg gaatacatca ttctgtcccc gctttttgaa tcggggaaaa
      121 gcagcccgat tctggaagaa gtttttgtaa gcccgctcaa ggtctttcag tgactgttgc
      181 aatggctgtg acggggcatc tttaagccat tgcgtttcag tggcattttt ccactcaacc
      241 agccaggaag ccattttccc gtaagggatg tatttattac cgacctcatg attctcattc
      301 tgacgtgcca gagcacgatt gaaaacgaaa cgacatgcgc ctgcgaagcg cctcatttca
      361 cgctcctgtt gaccaccggg tcttaactgg aatttaaatg cttgtagtcg tttcacaggc
      421 caaatgatac tttggtctgt gaaaaaagaa accgatatcc ggcgtggaag gcattgtgtt
      481 ttcctgatgc atgttcacct ggtctttgtc accagatacc gacgtcagat ttttgaccat
      541 gacgcgacag aaaaactacg cacttacttt tcaaatgtat gtgcttattt tgaagctgaa
      601 ctggttgaaa tggatggcga accagatcac gtccatttgt taattaacta acctcccaaa
      661 ctggcgatat ccagtctggt aaacagcctc aaaggcgtat cgggtaggtt actgcgacga
      721 gatcgaccag atattgcagt caggtattac tacaaaggcg ttttgtggag tcctggctat
      781 tttgccagta gctgcggagg tgcgccaata tccgtcatcc gccaatacat tgaacaacag
      841 caaacacctg gtcaggtgga aaaccgcgcc ttatatcccc gccctgaagg atggggtttt
      901 acggcgcacc ggataaaaag cgccatctaa cgatggcgcg tctggaacta ctttttgtta
      961 acgtcaaaca tggtggcatc ggtcgccatg tcatcaataa cttgtttaat attttcgaaa
     1021 gccattggcg tactttcgtt attcaggtct ttaccttcgc cctgacgaac gactttgata
     1081 accggtttat tagtcgctgc atcaatcagc tcaccttcaa aatagaggcg agtatccatg
     1141 gtacggtggc ctgtagccat ttgcgtcccc gcaaccacta atgcaatagg aaccacttca
     1201 tagaattgca gcccttcttt gctggtatct acaccggtaa tggccccacg gaaaatcaga
     1261 ctacgcggcc cagcggtggt aaccactggt ttacgctgcg ctatcgcttc tttcatttcg
     1321 gtgttggtat agttcaaaat tttatccaga actttctgcc ctacctgggt agaaggtttt
     1381 ggtaccggat aataagtgat tgggttccag acgatgctgt catatttgct ttgatcaaaa
     1441 ctcgggtcta cccaacgtaa aacaggttta cctgttgctg aggttgtttc ttttaaatca
     1501 gagtaattgt ttaaaaaacc agaatattta tctggctggg tgattttaga ggcacaacca
     1561 gacagagcca acaagccgca taaagctgca actttcgcaa atgatgtggt acgcatgaat
     1621 agtttcctga gatgattata ttactttcta agttatagca aaaagcgccg cttcgtgttg
     1681 tggcaaaaaa agtgttggtg aaaataaatt tttcagcccc ttttcagacg acatagtcaa
     1741 tgccgaaaaa ggggctaagt caatcatcag tagttaaatt tcagtaagtt aagctgctaa
     1801 aaacttcgac tactgtgatt aatttcacat cccttcgata aaaaatatgc ttaccgaaaa
     1861 gaagaacagt aaatcatctc agcgtgggga attttcacgc gttttgtgct taccgggtca
     1921 ttttttacgt gccagcatcg tggcgaaacg cagtttaata cgattaccgt tggcgtcggt
     1981 gcggtgcagc tcgccgacgt cttcattgta tttcaccctc tcccagcctt cataatatcg
     2041 acgtaattct ccctctttga aggcaaacgg gaagccgacg gtacatggat aatcagcggt
     2101 atccatcgcc gccacaatca ggttgtaacc accaggttta gtgcaacgtt gcatattggc
     2161 aatcagccct gggatggttt tagcctcaag gaacatcagt accacagtcg aaagaataaa
     2221 atcgtactgt ctatcaaatg tgaggttatt cagatcaacg acccgggtgt gtaaattatc
     2281 cagattttca atggatttaa tgcgctctac attggcgata ctcatagcat ttttatccca
     2341 tgcgtcaaca tcataaccat tggctgccag gtaaagactg ttacgaccat tgccacagcc
     2401 cagatccagc gtttttcccg gtttaaccac tttcaccgct tccagtactt cagagtgtgt
     2461 gcgggttaat tcatatttat cagtaaaata gttttcgtca cgaatgatca ttctttgtcc
     2521 tctgctttca ttaaaacggc gcgctcggtt ctgactaaca attttccctg catcagaagc
     2581 gcaaaagtac ggatgagcag tattgcaata ataaaattgg taaagataaa cagcggtacc
     2641 gccagcgtat ggaaaaatcc attttcgctg ccactcccca gatgcaaacc ggtggttgcc
     2701 agtgcagata cgccgaacga gaaactccag aatgaagcat taaatggctg ggagagatac
     2761 catggcatca gacgtagcat aaacagcagt tgcagcagtc cataaccaaa aagcattttc
     2821 gccagcgtat caccctcgcc gccgttgacg ctcaaccagg cactacaggc caccagcgca
     2881 ggagcgagct gaatgccgag tgatgtccgc agtgccgtgg gtaattctcc cgaactgcgc
     2941 agacgctgca agatcacagg ttcaaggctt agccatgaga aaacgcctgc gcctaaaaac
     3001 accagaccgg cgtcggtgta gcccaacgca ccacaggcca ttgcgctgat aaagttgttg
     3061 gcaactgtcg gcagatacag tccaggcgtg gtagcttctt cagggtgaga tccgcgccat
     3121 aatcccgcag tttgccaggc ggcataagcc aactgaacca cgacaccaaa actgaacagg
     3181 cacaccgcca gtgggcgaaa ccacggaaca aaaccaatcg ccaccagcat cgtcgttgcc
     3241 ggaaacaaac tcacaaaact gctcatcact ggatggcgca cttctgccag cacgctatgc
     3301 ggaaagcgta taagtcgggt aataaatgcg ctagttaaca atccccagat gatcatcgcc
     3361 agaatcacca gcccatcccc taaccagtgg ctgacctgcc aaacctggct ggcatagcgc
     3421 caggcaaatc ccatccctat cgtccccaac acaataccaa agtagcctgc cggcaaattg
     3481 agcactttat cgctctgcat cttcttactc attttgttta atttataaag tatatttgaa
     3541 atgcatttta tggaattttc tcgagcatag ccagagccgc agaatttgct acggttatag
     3601 taagtcacct gtgggaaaac gctatgcgca aatatcgtct aagtgaagaa cagcgagcct
     3661 ttagttatca ggaagatggc actaaaaaaa atgtgttatt acggcagatt atcgccatta
     3721 gcgattttaa cgatgtgata gcaggaaccg ctggcggctg gatcgatcgc gaaacggtgt
     3781 tagcacagga aggaaattgc tggatttacg accagaacgc tattgcgttc ggcggagcgg
     3841 tgatttccgg taatacgcgc atcaccggca ccagcgtgct gtggggagag gtttacgcaa
     3901 cggataatgt ctggatcgat aatagtgaaa tcagccaggg tgcatatata agtgacagcg
     3961 tcaccattca tgactcactg gtttgcgggc aatgtagaat atttggtcac gccttaattg
     4021 atcaacattc tatgatcgtc gccgcacaag gcttaacgcc cgaccatcaa ctcctgctcc
     4081 aaatttatga tcgcgcaagg gttagcgcct cgcgtattgt ccaccaggca caaatttatg
     4141 gcgatgccgt cgtccggtat gcttttattg aacatcgcgc cgaagttttt gattttgcca
     4201 gtgttgaagg taatgaagag aacaacgtct ggctatgcga ttgcgctaaa gtctacggtc
     4261 acgcgcaggt gaaagctggc atagaagaag atgccattcc gacgattcat tacagttcgc
     4321 aggtggcgga atatgccatt gtggaaggta attgtgtgtt aaaacatcat gtcttgattg
     4381 gcggaaatgc cgtggtacgt ggtggaccga ttctgctcga cgagcacgtt gtaatacaag
     4441 gtgagagccg tatttcaggt gcggtgataa ttgaaaacca tgtggagtta accgaccacg
     4501 ccgtcgttga agcctttgat ggcgacacgg ttcatgtccg tggtccaaaa gtcattaacg
     4561 gcgaggagcg cattacgcga acgccgctgg cagggttatt atgaatcgat aatacgcgcg
     4621 tataagttca catcatcata ggcatcattc aggaactcag cctgtttcag gcaaccttca
     4681 aggataaaac cattgcgcaa agcgacctgg ttgctttgcg gattgtccac ccgacatttg
     4741 atcacgaagc gtctaagttc accagactga gcgtaatgat gaatcaatgc ctgcagcgcc
     4801 tgagaaatga tcccctgccc ctgatgagat tcgtccagcc agtagccgat ttcagcagtt
     4861 ttactcagtg gttcaatacg attaaacgag ataacgccga taagttcatc ttctttgaaa
     4921 atcatgaaca ttttggcata gccgcgttga tgcaacatca cattaccctg caccgttttt
     4981 cgcgtgtcct cttcactctg aacgaactgc ggccagttta gcgactgctg taaccaggtt
     5041 ttatttttac agattaactg ataaagaggt gtgacgtgac tttccgcaac agcatgtaat
     5101 tcaagtgatt cgcttacttt tatcgtttca gtcatcttat ctccagtcaa tccatgctgt
     5161 tgttaaaaaa gccgcctggc tggcggctta ggaaagttta cacctctttg acgctctcct
     5221 gctgcaggat tttgagcatc tcatctttta actgtagctt ttgttttttc atgcggacca
     5281 cttccgcatt gtaccctcga ccgtcggaac cttcctttct ggcaatttca tgatcaagtt
     5341 tattgtgttt atcgaacaag gacataaagc gaggattttc gtttttcaga cgggatatta
     5401 aatctcggta ttctggaaac atacgcactc cctgttagtg gctgacaagg tgtatctgaa
     5461 atacacacca tcagattact catttaactt agggaaaaat gcgagcggga tcgtgtttaa
     5521 ccagacggca cctcccgaag aaggtgccga agaaaaaacg attaactcat cacgcggtcg
     5581 tcaacatact gacgtttatc cggcgctggc gggaaatact gatacagcca tgtttcactg
     5641 atagcgtcgc cctgacaacg cagatacatc cgcatatcga ccggatcagt ggagtccgaa
     5701 gtcggatacc agtcaaactg aatacgataa ccatcaatgg gttcaatata gagaatttcg
     5761 atttgcttcg cttccccact ggaaagcgta atcaccggct caatgccttt tggtgcggca
     5821 gctttcagat caccaccaac gaaatcgacg gcaaaacgac gcgcccattt ttcgggatag
     5881 tgttcacctg gtgcccaacc ttccgggaaa ccgcccatgc cggtacgcgt cgccataacg
     5941 cgcgctaatg ggcaatgaac aggcggttgc gcactccagt acagacgata ctggaatgca
     6001 aactcatcac ccgcctttac agctttttct ggctgccaga agcagacaat gttatccagc
     6061 gtttcgcccg ttgttgggat ttccatcagg ccgatggtgc ccttacccca cttgttacgc
     6121 ggttccaccc acagacttgg gcgtttgtta taccagccca taatgtcctg ataatgggag
     6181 aaatcacgat ccagttgcaa taaaccaaac cctttcgggt tgttgtcggt gtaagcattg
     6241 aactgcaatt tttgcggatt attcagcgga cggcaaatcc actcgccgtt gccccgccac
     6301 atggacagac gatcagagtc atgaatttgc ggatgaatgg tgtcgcacat ccgacgttca
     6361 ttagtaccgc agctgaacat actggtcatc ggcgcaatgc ccagctgttt aatgtctttg
     6421 cgcgcataca ggtgattttc cacatccata atcacctgac ttttctcaca atggatagtg
     6481 aacttatagg caccagtaat gctggcgcta tcgagcaacg cataaacggt aaaggtagtt
     6541 gcccccggtt ttaccgtatc aaaccagaag gcggtaaagt cggggaactc ttctttactg
     6601 tcggtgtaag tgtcaatcgc caggccgcgg gccgacaaac cgtattgata tgtgtcatca
     6661 acggcgcgga aataactcgc tccgaggaat gatactacat cacggcgcgc cagttcgggg
     6721 gctttaaaca cgcgaaaacc ggcaaagccg agatcgcttt gcccttctaa ttgtttggta
     6781 tcaacacctg catcgttgta tttgaacaac tccgggcgaa agtgaatttc acgcgccaga
     6841 tgtgttgctg gatctacaga aaacatacga acgcggcgac ggaatcccat tcccatatgg
     6901 aagaactgag cgtccagttg acggttctca acgttatgcc agagcgattt ttcggcgtcg
     6961 tattgaatac tgttataagc ctgcggcgtc attgtcgcca gcgtgtcagg taacggacga
     7021 ggcgcaccac gccacgctgt ttgcgctaag tcgtgcgcca ttgactgtag aatggagaag
     7081 tcaaaacgct gggtttgccc gtcggcaata tcagaatctg ccgcgaatgc cgcctgagaa
     7141 aaaagagaag caatgccgct ggtaccgcac acggcggcca tagccattga acctttaata
     7201 aatcgtctac gatccatacc tgaaagtgag tccttctggt gttgtgaatg gtcccgcgct
     7261 ggtcgttttc agcggcaaga aagtgcttat ttatgaccgc tcactctaga caatatttat
     7321 taataatcca attgttgaac tcagagaata tgcataaatc ggcaattttt ttatgtcgat
     7381 gggatgagcc tgattcgttt gtaaaatcag gcaaattttt gcaaaggcgg atggtcagaa
     7441 tttaaagcaa accacagcga cgtttactgc gttcttccgc ttcctgataa agctggaact
     7501 catccaaaac cggacaaccc aacgcctgct caaatgcctc gcgactggca ttaccgtgat
     7561 tgcgtgcctg cgtttcatta cccagatttg actggaagat ccccgccgcg ctaacgggca
     7621 agaaatcttc ataggtaatg ggttgcgccg cgacccaacc acgttcaatt aagggctgtg
     7681 gatcgtctcc aggatgaatc gcctgacgat gcgcctcacc tgaaggcgtc agacggtacc
     7741 ggaaccatgc cagcccttgc tgacgcatta aaaactcact gtcaggaaaa gtgcggaagg
     7801 tttcctgtaa atgcatttgg tgagtgagat tatcctgccc ggttccagcg ttacgcagaa
     7861 gatcatcata cagttgtcgc cctttcggcg ttaatgccac gccacgctgc tcaatttcac
     7921 caaagcgcgc ggtatgcgtg ccctgtttct gccccgcaaa caacaccgtc tcttccagtg
     7981 ctttaaagct ggtctggcgt agtaaaatcg gtacctcgcg gcgcggcggc ccctcgatga
     8041 gaattttggg ttcaattccg cattcaggca tcatcgactg cacccggtca atatccagcg
     8101 tacgtggcgt caggtggttg atatggcatc caggaaaaca gaccacatca gcaatcaacc
     8161 gatgttcgtt gtgcaaggcg cgataggttt cttcatctac cgttgctgac tggtgccagc
     8221 gaaacgtttc cagggcttcc tgcacaaact cctgtgcctg tgtttcgtta aaaccgccct
     8281 gctgctcata ttcctctaac agttgtcgac aacgtggggt gaagatatcg cgctgacgca
     8341 gaatctccgc cgctttctgg cgcaaaattt cgttctcgat aagctcaagg cggagtaagg
     8401 aggtaaaaac gcggaaggga ttacgcgcca gagaagcatc atcaatgggc cgaaatgctg
     8461 tcgaatgtac cggcacccct gcctgcgaga gatcgtaata gctcaccggg tacatcccca
     8521 taatggcaaa catccgccga agagtagcaa gctcttgtgc agtcccaacg cgaatcgccc
     8581 catgacgttc aacattcagt cgcgccagct cgtctgcatt taccattttt tcgtgcagtt
     8641 gaggattgtt ttccagcaca gccagattca catcagctac cagttccagc agcgtgccat
     8701 attgcggaac ttcttgctgg tacatggctg acattgcctg cgaaaactgt tcccgaatct
     8761 catccgccgt gatgctgttc gccatagtgt ctgcctccag tggtcagtaa tctggagtgt
     8821 aggtaaccgc attcactctg gcgggaagaa tttacaaact gtgatctcgc cgcgaaaaca
     8881 tcaatattat ccattttgct gtaacataat tgctttaatt gttaataata ttttgcaatc
     8941 aagttatcat aatcaaacaa cttcacttgt cagcgacacc gcttcgtttt taacatcgct
     9001 tatggaaaaa aatagtctgt ttagtcagcg catccgtttg cgccaccttc atacattcgt
     9061 agctgtcgca caacaaggaa ctttggggcg cgcggctgaa acccttaatt tgagtcaacc
     9121 tgcgctctct aagacattga atgaactgga gcagctgact ggcgctcgct tgtttgagcg
     9181 tggtcgtcag ggggcgcaac ttaccttacc cggcgaacaa tttttaacgc atgcagtcag
     9241 agttcttgac gccatcaaca ctgccggaca gtcgcttcat cgtaaagaag atcttaataa
     9301 tgatgtcgtc agggttggtg cactacctac tgcggcactg gggatattac ctttggttat
     9361 aggtcagttt catcagcaac aaaaagagac gaccttgcaa gttgcgacaa tgagtaaccc
     9421 tatgattctg gcgggtttaa aaaccgggga aatcgatatc ggcattggtc ggatgtcaga
     9481 tcctgaactg atgaccgggc ttaattacga actgctgttt cttgaatcgc tgaagctggt
     9541 tgtccgccct aatcacccgc tacttcagga gaacgtaacg ctaagccggg tgctggaatg
     9601 gccggtcgtt gtatcaccag aaggcactgc gccacgccag cattcagatg cattagtgca
     9661 gagccagggc tgtaaaattc cttcgggttg tatcgaaacg ctgtctgctt cgctatctcg
     9721 tcaacttacg gttgaatacg actacgtgtg gtttgtccct tctggcgcgg taaaagacga
     9781 cctgcgtcat gccacgctgg tggccctgcc tgttccggga catggtgcag gcgaaccgat
     9841 tggaatactg acccgcgtag atgcgacgtt ctcttctggt tgccagttga tgattaacgc
     9901 tattcgaaaa tcaatgccgt tctgaaaggt gaagggatct gtcgatccct ccttgaacat
     9961 tttcacaccg tagcgaaact aactggttca cccgctccgc gaggttctgc cgacacagaa
    10021 tgtttgttca gacggaatac gtccaccgcc tcagttaatc gtgccgcctg ttcttcaagg
    10081 gacaccgccg ctgctgaggc ctcttctacc agagaagcat tctgttgcgt caccttatcc
    10141 atttcagaaa tcgcctggct aacctgagtt atgcctctac tttgttcatc cgaggcggcg
    10201 gcgatttcct gcatgatatg tgtgacactc gcgacggcat caacaatagt gctcatggtt
    10261 ttccctgccg ttgccacctc atccgacccc aggtcaatta acctgactga ttcactgatc
    10321 aagccttcaa tctctttcgc cgcttgggcg ctgcggcttg cgagtgtccg tacttcgctg
    10381 gcgacaacgg caaatccacg gccttgctca cccgctcgcg cggcttcaac ggcagcattc
    10441 agtgccagaa tattcgtctg gaaagcaata ctgttgatga cggcggtgat ctcggaaatt
    10501 ttcttcgaac ttgtagagat agcgcccatc gtttttacta caccggaaac cgtctgcccg
    10561 ccatcgctgg ctttaataga agcctcttgc gccagtttgc tggcatgatg cgcgttatcc
    10621 gcattctgtt tcaccgtcgc agtgagttgc tccatgctgg cggcagtttg ttcgatagcc
    10681 gccgcttgtt cttcggtgcg agatgacagg tccgcattgc cagctgaaat ttcgctggtg
    10741 ccacgataaa tctcttccgc gccctgtcga acagtcccta ctgtcatccc cagtgaatgc
    10801 tgcatttgct gtaaatgacg acttaagcga ccgatttcat tacgacccgc aggttcatca
    10861 ttcatcgtca gatcgccact ggcgattttt tcaatccgtt gtgcggcatg ttgcagtgga
    10921 cgaatgacga tccgacgtag caccataaat gttatcagcg tcatgaccag ggcaagcaca
    10981 aacgcgccaa tcatgaacat cccacccagg cgggtgcgct gatgggccag ttccgccagt
    11041 tggttggctc tggtgctacg tatcttaacg gctttgttca aaatatcggt ataagcatta
    11101 tccagcggtc ggatctgctc actttcatga ttgataatcg cttcaaacat gccatttttg
    11161 gcatatttca acataggttg catacccgtg atataagcct gaaagcgttg atttaattca
    11221 gtgtcgaggg cttcatcagc aggtgttttc accggccgat tctgataagc acgataacct
    11281 tgctgcgact gtttaatctc cgattcggct tgcgcaatat tgcgcttcat tgcttccatt
    11341 tccgcaatac gactcgcagc cccggcctga atcatattga tccgggctga acgcaaaaaa
    11401 tctgaactgt tcgctaaccc ggtccgaatc tcaatctccg cagtaacatc acgctgatcg
    11461 cgatcggcct gccagaggaa atagccagcc agagcagaac ttaatgcgaa tagaactaag
    11521 ataccgccaa gaatgcaggc aaataacgga accagcctga tgtgatgcaa aaaacctaat
    11581 cgatgtgagg gagttgtatt cattaccgtt gtctctcgtc caggtttact cctgatagaa
    11641 agtcatcggc acttgacggt aattacttat gaccaaaaac ctctttgcgt cacattttta
    11701 caacaattat tgcagagaaa attaatgcta ttggaatgaa gagagtaacg aatggctaaa
    11761 ctggctaacc cgaatgacga aaaattctgg aaaacagcac gcaatttcgt gaagtgatta
    11821 ttttcgtccc taaggaaacc tcataggatg cctgcgggtg cctgagactt tctgttttca
    11881 ggctaaccgg gaatcagaaa gacgaaggcc ccgaccgata tttctatcaa ccgaggccaa
    11941 cgaatgaacc tagcaattca gatcttagcc tcttacccgc catccggcaa ggagaaaggc
    12001 tatgaagcac aaccctctgg tggtgtgtct gctcattatc tgcattacga ttctgacatt
    12061 cacactcctg acccgacaaa cgctctacga actgcggttc cgggacggtg ataaggaggt
    12121 tgctgcgctc atggcctgca cgtccaggta agggcaagcg cggggatttt ccccgcgcat
    12181 ctgactagca gattcatttt ctcatggcac cccttttaca cttcagttag tgcatgtttt
    12241 tttcgaggca ctaatagaac catcattaca gacaaatttg ccgcctgccg tacagtgtga
    12301 gatcccccct ttcgaaccag aacagggttt gcgcccggca tcggcataaa aggaaacaag
    12361 cgttcccata aacaaaataa gtgccagttt tttcatataa cctccatggt ttatcgaatc
    12421 ttatgttcga tttgaacgtt atacgctggt ttattttgat accgttgatg agatcaaaga
    12481 tcgggtgaat tgattaaaaa taagccagat ggacgtatgg gcaaggatta tggttttatt
    12541 tgtcatacaa ataaatataa taggcgcttc cgatgtagac ccgtattctt cgcctgtacc
    12601 acgggtcggt tttagtacag gcgttttctt taaatatcct tcaggaacgt ttacgcggca
    12661 tcatgcgtag aagtgtgttg tccttccaga aatagtggtg tgccagtgca gccgcagcgt
    12721 gcaacccgat gacaaaatat cccagattcg ccagcgtctc atgccacgac tttaagctat
    12781 caacccgttc gaaattggcc tctgaagcgt aaggcatcgt caaaccaaac gcaaaccacg
    12841 ggttgccccg gttatacatc atcaccaaac caatcactgg cagcgcaata aacaggagat
    12901 aaatcaccaa atgtcccaaa tgcgccagtc ccgtcatcat cggttttggc ttaggtataa
    12961 tcggcggggt tgggtatttc agtcttaaca aaagacgaac caccatcagc acgagaattg
    13021 agataccaca ggaaacatga accatgttga tgagtggccg atcgctacgt gggaagaaac
    13081 cacgaaactc cattgcgcaa tacgctgcga taaccagcaa aaagaccagc cagtgaatgc
    13141 tgatttgtaa ccttgagtat ttattttcca taacatttcc tgctttaaca taattttccg
    13201 ttaacataac gggcttttct caaaatttca ttaaatattg ttcacccgtt ttcagtacgt
    13261 tacgtttcac tgtaagaatg gagattgccc atcccacaat cctggtctaa gcctggaaag
    13321 gatcaatttt catccgaatg ttcctgacag gagaaaacca tgagtaaagt tggtattaac
    13381 ggttttggtc gtatcggtcg actggtgttg cgtcgattac ttgaagtcaa aagcaacata
    13441 gacgttgtcg ctattaatga tctcacttcc ccaaaaattc tcgcctacct gctgaaacat
    13501 gattcaaact acggaccatt cccctggagc gttgatttta cggaagattc acttatcgtt
    13561 gatgggaaaa gtatcgcggt ttacgccgaa aaagaggcta aaaatattcc gtggaaagcg
    13621 aaaggtgcag aaatcattgt cgaatgtact ggcttttata cctccgccga gaaatcgcag
    13681 gcgcatcttg atgctggtgc gaagaaggtg ttgatttccg cccctgccgg tgaaatgaaa
    13741 actatcgttt ataacgtcaa tgacgacact ctggatggca acgacaccat tgtttccgtg
    13801 gcgtcatgca ccactaactg tcttgcgccg atggccaaag ccttgcatga cagtttcggg
    13861 atagaagtcg gcacgatgac aaccattcat gcctatactg gcacccagtc actggtggat
    13921 ggcccacgtg gtaaagattt acgtgcttca cgcgcagcgg cagaaaatat cattccccac
    13981 actacggggg cggcaaaagc cattggtctg gtgatcccgg aactgagcgg caaactgaaa
    14041 ggtcatgcgc aacgcgtgcc ggtgaaaaca ggttcggtca ctgagctggt gtccattctc
    14101 ggaaaaaaag tgactgccga agaggtgaat aacgcactta aacaagcaac caccaataac
    14161 gagtcatttg gttataccga tgaagaaata gtctcttccg atatcattgg cagccatttc
    14221 ggttcggtgt ttgatgccac gcaaacggaa attaccgccg tgggcgattt acaactggtg
    14281 aaaacggtcg cctggtacga taacgaatat ggcttcgtca cgcagcttat tcgcaccctc
    14341 gaaaaattcg ctaaactctg acgcgcacag gcggaggaaa aaacctccgc ctcttttact
    14401 cattacgact gtaaataaac cacctgggtc tgcagatatt catgcaggcc atgtttacca
    14461 tctgcgccgc caataccgga tttacgccat ccggcgtgga agccttgcat agcttcgaag
    14521 ttttcacggt tgatgtaagt ttcaccaaac ttcagccctt taatggcttt catcgcgacg
    14581 ttcagatttt gggtatagat tgatgaggtc aggccgtaat cactgtcatt agccattgag
    14641 atagcatctt ccagcgtgtc aaatgcgacc accggcagaa ccgggccaaa ggtttcctca
    14701 tgcataatcg acatttcctg gcgaacatcc agcagcaatg tcggcggata ataatatcct
    14761 ttcccgtcta ctgctttgcc acccaacgcc actctcgccc cttcttctac tgcgcgcgcc
    14821 actttttgct cgaccctttc cagcgccgcg gcgttaatca acggccccat cgcaatgtcg
    14881 ttgcgttcag cggggttacc aaattgaacc gcctgcatcg cttcacccag ccgattgacg
    14941 aactgatcat aaatgccttt ctgtacataa acacgttctg cacagttaca cacttgccca
    15001 ctattaatga cgcgtgaatc aacgatggct ttgactgcca gttcaagatc ggcatcgtcc
    15061 attacgatag ctggtgcttt accacccaat tccaggcaca ctttggtgat gtttttcgcc
    15121 gcagtcgcca taatcttctc acctgcagag acgctgcctg tcatactgac cattgcgacc
    15181 tttgggttac ccgccagttc ttgcccaacg gtttcaccac gccccagtac aaggttaaac
    15241 acgccgcgcg gaaggcctat ttcatcgacg attttggcga atgcaatcgc attgtttggc
    15301 gtaaattcac tgggtttaat gacgatggta ttaccggtca aaagagcggg agccattttg
    15361 cgggcaatga ggaagaacgg gaagttccac ggcagaatgc cggtagtcac accaagcgca
    15421 cgtttaaaca aaagaatatt ttctcctgga cgatcgcttt gaataatctc gccctcgtaa
    15481 cgccgtgccc actccgccat gtaatcgata tagtcggcag taaaagccac ttcgacttca
    15541 gccagctgct ggatcttgcc cccttcttca acaatcagcg cactgatttc actggcgcgt
    15601 tcgcggatgc cggcggagat tttgcgcaac caactggcgc gttcaatagc aggcaacgct
    15661 tcccattctg gttgtgcacg ttctgctgca tcgattgcct tacgggcatc ctcggcctga
    15721 ccatcgggta tgcgggaaat gacagcctct gtagcagggt ttaccacatc aatccatgcg
    15781 tctccacgcc aggtaacaaa ctgtccatcg atatacatag gatgttgaac gggtactgac
    15841 atgggcgact cctgtgattt atatgtttgt tttcggtgaa tgcattgtta attaacatca
    15901 atcatcagag agacggcatc gtgaaaggac aattctgtga agggcttcat aaaaacaagt
    15961 gtaaaaggaa taaaaggcag aaataaaagt caggagtcat gcccgtttgc aaacaagtgg
    16021 ttcacaaacg ggcaattttt aacgtaacga gcgactttcc atcgcctcga tgagcaccgc
    16081 atcatgtttc agcgtttgcc atgcatggat gacttctgcc ggaaaatcaa cgtgaacgat
    16141 aaaatctcgc ccgcggggac cgtagccatc gctatcatcg cgtaaacgcg gcagctcgcc
    16201 agtgagtagt gagagataac gctcaaccgg ccataatcct tgtaactgat taataaagat
    16261 tacactgtct gcgttatttc ctaactgagg aacgaatccg ggataactta accagcgtgg
    16321 tgcatcggga ttgtccccag tcatacggcg gaacgtcgcc atcgtgcgcc gctgcatggt
    16381 ggggtcctga acaacgatag ccgtatgaac tcgttctacg gcctgattca atagcgcgat
    16441 gctaaagcgt gcgttttcac cgcagtttgt tgactggtct tcaatccaga ttttttcatg
    16501 cggaatgtgc cagaactgat gagcgatatc cgccaggatg gtcgcttctg ctctgccagt
    16561 ggtgcggata gtgttgtagt gcggatgctg tgcgatggcg ctatacaaaa aagttgtcga
    16621 gtgaccgata ccaccactaa tcagtaaagg aatttgctga tcgcgagcaa tcttacatgc
    16681 cgcgtcgata gtcggcataa ccgcattgcc tgcaaggatc acgcaatcgg cctgatacgg
    16741 cacctcaccg gagaaatcat cctgcgccag ccactgtccg ataacattta tggcatctat
    16801 agttgccggc gaaagcgtcg gaaacggggt aatgttcatg gttcctcctt atcgttccct
    16861 taagaatact gcgtgagata atagtgagaa gcggagcact ctgaaaggag gaagtcgtat
    16921 gtccttaacc gccactccgc aagcctgaca tatcttttaa ggaaatgctg tagataaaca
    16981 ttattcatct tgcagcagag aattgtattt ttctgaattc ctgaattttt acccttaccg
    17041 ttttagaatt aataaaaaac ccggcgctaa atgcttaccg ggttttttct ttatcaagca
    17101 aatagcaggg ttaaccgcta atctgctcca tcgcctgcaa aatacgctta tctgaaatcg
    17161 gataaggcgt accaagttgt tgagcgaagt aactaacgcg caactcttct atcatccaac
    17221 ggatctcttt cacgtcttca tccttacgac gtgcgggcgg cagtttgttg atccattgct
    17281 gccacgcctg ctggacgttt tcgactttca gcatctgagc acggtcgcga tgtggatcaa
    17341 ccgccagttt ttccagccgt ttttcaatcg cctgcaaata tcgcagcgtg tcgcccagcc
    17401 gtttgaagcc gttaccagtg acaaaaccgc gatataccaa cccgcccatc tgcgctttaa
    17461 tgtcagaaag ccccagtgcc atggtcatat ccacccgccc tttcagacgt ttgttgatat
    17521 tgaacaccgc agtgaggatt tgctcgacct gcttcgcaat atccaccacc gtgtcattca
    17581 gttcggcgcg cactttttca tgcagcgcag caaagccttc ttccgtccag accgggccac
    17641 cattggcgtc gatcaattga tccacaccgc aggagataca gtcgtcgatc agctccagca
    17701 ctttgccata cgggttaaag tacagtccca gcttggcttt gttcggtaac ttttcatgta
    17761 aatatttgat tggcgatgga atattcagca gcagtagacg gcgaagaccg ttccacattg
    17821 cctgcttttg ctccagcggg ttatcaaaca gtttgatcgc cacactgtcg cgctcatcca
    17881 ccagcgccgg ccacgccttc actttgtagt tgccacgctt ctgttcgtag ctttccggca
    17941 gctgaccaaa actccagata tgtaagccgc tctgctcgat accgtcatcc gccaccgcag
    18001 atagcgtttc ctgcactttg cctttcagcg catctttcag atcttgtagc gagcgccctt
    18061 cttttagctt cttgtttttg tcatccacca cgcggaaggt aattttcagg tgatcaggca
    18121 cctgatccca gtgccagtct tcgcggtcaa cggtaacgcc ggtcatccgc cgtaactcgc
    18181 gctcaaggct gtcgagcaac ggtaactcca gcggtttgac gcggcctaaa aacgcttcgg
    18241 catagtttgg cgcgggtaca aaattacggc gtaccggttt cggcaacgat ttaatcagag
    18301 caatcaccag ttcgcggcgc agaccgggga tctgccattc aaacccgctt tcctcaacct
    18361 ggttaagtaa cggcagcgga atatgtacgg tcacaccgtc agcatccgcg ccgggctcaa
    18421 actgatagct caaacgcagc ttgagattgc cctgatgcca gaagttcggg taatccagct
    18481 tgctgatttt ttctgcgccc tctttgatca acatgctttt ttcaaagttg agcaaatcag
    18541 gggtttcgcg gctgactttt ttccaccagc tgtcgaagtg acgagcggag attacatcgt
    18601 ggctgatgcg ctggtcgtag aactcaaaca acgtttcgtc atcaaccaga atatcgcggc
    18661 gacgtgattt gtgttccagc tcttccactt cggcccgcag tttcaggttt tcacggaaga
    18721 atgcgtgacg cgtctgccag tcaccttcca ccagcgcatg gcgaataaag agttcacggc
    18781 ataacgccgg atcgatctgg ctgtagttga ccttgcgcgc ggcaacaatc ggcaaaccat
    18841 aaacagtgac tttttccgtt gccatcaccg cgccctgcgc ccgttcccag tgaggttcgc
    18901 tgtaggtgcg tttaatcaaa tgctgagcga caggctccac ccattccggg tcgatacgcg
    18961 cagcaatgcg cccccacagg cggctggttt ctaccagttc cgccaccatt acccatttcg
    19021 gcggtttttt gaataaacca gaaccgggaa agatggagaa acgcgcgtta cgtgcgccgg
    19081 tatattcttg tttatcggca tctttcatgc cgatatggga aagtaaaccc gtcagcaacg
    19141 caatgtgaat ttcgcgatac tccgccggtt cgctgttaac cggaatgcca agttctttca
    19201 ccacctggcg caactgggtg tagatatcct gccattcgcg cacgcgcaga tagttgagat
    19261 aatcggtacg acacaggcga cggaaggcgt tggaagaaag cgccttttgc tgctcgccaa
    19321 gataattcca cagattcaca aacgcgagga agtcagactc tttgtcgtgg aagcgacgat
    19381 gtttttcgtc cgatgcctgc tgtttatcca tcggacgctc gcgcggatcc tgaatggaaa
    19441 gcgcggacgt gataatcatc gcctcacgca cgcagccatg tttttgcgct tccagcacca
    19501 tacgcgccag acgtggatcg acaggcaact gcgagagctg gcgaccgagc ggcgtcagtt
    19561 tataggcgct ggcctgttca tccgtggtga tcgcgcccag ttcttcgagc agacgcacgc
    19621 catcctggat attgcgttta tccggtgctt cgacaaacgg gaacgcagcg atatcgccca
    19681 gccccagcgc ggtcatctgc aaaataaccg aggccaggtt ggtacgcaga atctccggat
    19741 cggtaaactc cgggcgcgaa aggaaatcgt cttcggaata gagacgaata cagatccctt
    19801 cggacacacg cccacagcgg cctttacgct gattggcaga cgcctgcgaa atgggttcaa
    19861 tcggcaaacg ctgcactttg gtgcgatagc tgtagcggct gatacgtgct gtaccggggt
    19921 cgatgacgta tttaatcccc ggtacagtca gcgaggtttc cgcgacgttg gtcgccagca
    19981 caatgcgccg tccgctgtgc gactggaaca cgcggttctg ttcgctgttc gaaagccgcg
    20041 cataaagcgg caagatttcg gtatggcgta agttcagctt gttcagcgca tcggcggtat
    20101 cgcgaatttc ccgctcacca ctcataaaga tcagaatgtc gccagggctt tcctggctca
    20161 gttcgtctac ggcgtcaaaa atcgcctgca actgatcgcg ctcggtgtca tcggcttctt
    20221 caacaatcgg tcgatagcgc acttccaccg gataggtccg accggagact tcgataatcg
    20281 gcgcattatt aaagtggcgc gaaaagcgtt ccgggtcgat agtcgcggaa gtgataatga
    20341 tttttaggtc aggacgccgc ggcagcaact ctttcaaata gccgagcaga aaatcgatat
    20401 tcaggctgcg ttcgtgcgct tcgtcaataa tgatggtgtc gtactgcatc agcaggcggt
    20461 cttgctggat ctccgccagc aggataccgt cggtcatcag cttgaccatc gtgttatcac
    20521 ttacgtgatc gctgaaacgc actttgtaac cgatgcaacc gcctggctcc gttttcagct
    20581 cttccgcaat acggttcgcc accgttcttg ccgccagacg acgcggctgg gtatggccga
    20641 tcagtccttt aatcccgcgc cccagctcca tacagatttt cggtagctgg gtcgttttac
    20701 cggaaccggt ttccccggcg acgatcacca cctggtgatc acgaatcgct tcgagaatat
    20761 cctgtttttt ctgactaact ggcaaattgt ccggataagt gatttccggt cgtgccgctt
    20821 cacgcagcag gactttccct gccgcctggt caatctcttt cgccatctcc tggaaaatgg
    20881 cctgttgtgc atcaggattt ttaaccttct taacaccgtg cagacggcga gaaaaacgca
    20941 gtctgtcgcg cagcatcaac gaatccagcc gctgctgcaa agcagtaaag gtcaattttt
    21001 gttgttctgt cataacgtta gtgggcagta gtctgcctga ttaattttct tttaaatgat
    21061 agatatagag taccacatcg cggctttcta tgcgttgttc aataaattcg aacataggct
    21121 tcgatttatt gcgctatctc tgtggcagga ttgtgaataa agtgtcagca agcaacgggg
    21181 catcgcccat tcaaacatct ataaggaaac accatgagca aggtattagt tcttaaatcc
    21241 agcatcctgg cagggtactc tcagtctaat cagttgtccg attattttgt tgaacaatgg
    21301 cgcgaaaagc actccgctga tgaaatcacc gttcgcgacc tggctgcaaa tccgattccg
    21361 gtactggatg gcgaactggt tggcgctctg cgtccgagcg atgcgccgct gactccgcgt
    21421 cagcaggaag ctctggcgct ttccgatgag ctgattgccg agctgaaagc ccacgacgtt
    21481 atcgttattg cggcaccaat gtacaacttc aacatctcga ctcagttgaa aaactatttt
    21541 gacctggttg cacgcgcagg agttactttc cgctataccg agaacggtcc ggaaggtctg
    21601 gtaacgggta aaaaagccat cgttattacc agccgcggcg ggatccacaa agatggacca
    21661 acggacttgg tgacgccgta tctgtccacg ttcctcggct ttatcggcat taccgatgtg
    21721 aattttgtct tcgccgaagg gatcgcatac ggtccggaaa tggcagcgaa agcgcagtct
    21781 gacgcgaaag cagccatcga cagcattgtt gctgcataac cggacacgat cttgatgtgg
    21841 actgtagttc agtccacatc aagatccact tacctgtttt cccataattt cagcatcgct
    21901 ttgtgatctt ccttaagcac aatctgcggg cgtctggctc gaatatagct aatcgcttca
    21961 ttaacggttt tacagtgtcc gtaacataac aaccatgccg ccaccaccag cgcactgcgc
    22021 gataatccca atgcacaatg gaccagaacg ctgccttgct cttcgcgtaa tgtttccagc
    22081 atcgccacgg cctgtcggag ctccccctct tccggaacca ccagatccag catcggtaca
    22141 cagaaataga gtcgatcttt tgtcgctcgt ccccgaggga attcaaaggt gacgtccaga
    22201 accgcattct gtgccggaat atgtcgtgga aacgccccta aataaacacc agcagtaatt
    22261 ttgctcaccg gctccaggcg acgacaaaac caacgcatag acagccacat cccgatgcgc
    22321 cagggcaatg tcagccagta aacggcgggc gggagtttcc cctgactatc tttccctgtt
    22381 gttatcgcgc caagcccacc gtacccacgg ccaataatga gtagcgataa tactggccaa
    22441 cataaccaga ctgaccacca taactgaatc atcaccatta gctccatcaa cacaatgcac
    22501 gagcccgcgc ctacgacata tggcagtgct attttgattc gacgttgatc aggtttctga
    22561 taattccaac gacgatcgac gggcaccatc cagtcaatca acatacctac cgccagccct
    22621 gtgatgacat caataaaatg atgctgccag gtcgtcagcg tcgaaatggc gatgagtaaa
    22681 aaccatccgc cgcagacttt acgccacctc tcagccagat gctgacgaaa gtgacgccaa
    22741 agtagccagc agagaataat atgcagcgaa ggagactggt tataaggcag atcaaacagt
    22801 tcaagttgcg aaaatagcca tcccgtcacc ccactcactt caggacggat aaaactaaac
    22861 ttcagcggat agagcaaaaa accgcagcag gccattaccg ttgccagaat aagccggtgg
    22921 acaagtcggc gctgttcgaa tgtcgtgcta caaacgaaca gcgaaaatcc atataaaaga
    22981 tccagactcc agtaaggaac aatagtccag ggaagaaaag ggatcgccgt ttcccaaccg
    23041 aatacctgac tggggatatc atggctgtta aggtcctgaa ccgcggtgaa ctgattaaga
    23101 gatccatagg tgaagaagaa aaacggggcc agcaacaata accagccagc gccttgtagc
    23161 aatacgttcc gacgttcagt tatcacgacg caccgccatc gaaaccgtaa aaatacccca
    23221 ctcatcaatc cgttgtgtgc atttatcaaa tccggcatca cgcacgagtg aatccatctc
    23281 cccttgcgaa cgcacgcgca ttacccacgg tttaccatct ttatgactgg ttaacacccc
    23341 ggcaatcatc tccagttgag ggtgccacgg ctgcccggtg tagatgagaa tgccgcccgg
    23401 ttcgatggca ttggcaagac ctgcgagtga gttttttacc tgctcatttt cgggaaacag
    23461 ctcatacagt ccagagacaa tcgccagcgt aggccgcgga gttaacgcgc tgagttcctc
    23521 cgggttaaac gcatcgccct gttcaaaacg cacccgccca gacattcccc gttgggcaat
    23581 catctcttgc ccctgtgcaa catttaactc gctgtaatca cgtaacaaaa tatcgcttac
    23641 ggcaggctcg tttgccagcg catccagtac atagcgccca tgccctgcgg caatgtcaac
    23701 cacgcggacg gctaaacctt tggcgtggag atcggcaacg gcttgtttaa tcagtatttg
    23761 taaatgggtt ttacgctggc gaataccgcg ccaaccaata ctgttcaggt agattttgtc
    23821 aattaagcgc ccgaatgcgt tactgccttg cggttgattt tgatagacat aatccaggga
    23881 actgcctgaa tcaaagccgg tgctcattcc gagatgcagg cccgcagagt gcgccccgaa
    23941 caatttcatc gcctttcgca taaagcgata cgccaaatca accggcgata atggcacgga
    24001 tccacctgaa aggagccgcc agcgatccgc tgatggtcca gtgcggtctt catgctgata
    24061 atcaaatttt tgtgacttgt tagcatataa acgacgaata aagctttgca ttttttcaaa
    24121 tgcctgcgcc ctgttctctt cacccaacgt gtcgtgataa aagcctggca gcagatgcag
    24181 ctctttcaga gggctacgta atctctgata aaaatcaatc tgcggttggc gatgcaccac
    24241 atagtcatcg cctgatatca gaagttgcgt ggggagcgta atcgccgccg catctctaat
    24301 aatacgttca gacgttttat agagatcgag caagatattg acggcaatcg cccgtgtgat
    24361 cagcggatca ttattgaaac tcgccacccg ttgccgatcg tgggtcaaat agcgtccttt
    24421 cacataggaa ttaataaaaa acagaccacg cagacgatgc cataacgcca gcgcaggacg
    24481 tgccagcggc atatacaatt taaccttaaa ggccggagaa gccagcacca gcccgcgaat
    24541 tgcaggcgca taatcatgaa cccatgtggc aaccagcact gcgccgacgc tttgcgcgat
    24601 cacaaccacc tcttccagtc caacctggct gtcgctggca gcaaaacgga caaattcatc
    24661 gacatcctgc actgaacgcg caagagatgg gctataacca cgcggccccg aagtttttcc
    24721 atgcccccgg gcatcccatg cataaaaagc agtatctggc atcgccagtt catcaacgag
    24781 atgttgtaga cgaccagaat gttcatgacc gcgatgaaat aagacgatga cctttttcgc
    24841 cccgggctgt aaagcgggcc agtgccgata aaacaacgct gtattatcac tggtggtaaa
    24901 aaaatgttcc ccagggatgc gtgaattttc cattttctct attttccttc agtaacagca
    24961 gtagcgtata aaataaaaaa ataccggagc ggtgaaaatt aatgagtcaa tcctgtcaag
    25021 gagtccgcca tgtcctggta atagtttccc actatcttta acaccaatat ctcgtttgat
    25081 ggctgacatc actacatcgc cgcaaaaacc actaatacca attaacaaac ccgctaataa
    25141 tgcctgtaat gtatttagcg gtgtcagcaa cggtccaata atcagtgacg caatcatggt
    25201 ggtgatgacg ccccccacca gaccttccag cgtttttccc gggctgactt tggggaccac
    25261 ttttcttctg ccgcaggatt tcccccataa atactgtgca atatcgtttg actcggttaa
    25321 cgccaaaaga aataacacca gtaacgcccc gccctggata ttcatggatg gcaacattaa
    25381 taaccaggcg gcatgactca aggcgaaaac ggtcattatc cagccccaaa aaatagcgct
    25441 cacggtatgc agaaaaccgg agggttcacc cacaaaaact cgccaggtgg ctaatatcag
    25501 aaacccagac aaaggtataa ataacagaaa cagctcaaag caattaaaac cgataagcaa
    25561 gtagttaaga ggaataaccc aatataacca ccgcggaaaa tgtatggata taagcgtaca
    25621 atattctttc aatgccagaa aacttatcag cgcaaaaaac gtcaacgtca tccagcgtgg
    25681 acctgaaatc accagtgaaa aacaaatgac gatgccccac caggtacgaa tacgtaatgt
    25741 aacctcaccc ccttttctct ccggtaaccg ccatagcaga aaatggttta tcagcgttgc
    25801 taaaattaac aacgcgaaaa gtgttaccag agatttttcc agcattatct ttcagcactc
    25861 ataaaaagca cactccgaca acgattaatc gcagtccaga gaagcagaat tgaagcaatg
    25921 ctccataaaa gattattcca ttgcatccac tgtggataaa tggcaacggc cagcccccac
    25981 aacccaaata ttaacgcacg atcgcttttg ccgaatggac cggcataact gcgaataccg
    26041 ttaattgtct gcgcgagtaa accacaaaac tcagtcaata tggtgcaaaa taacatgagt
    26101 attacgagtg atgcgttact ttccggtaaa aataaaaacg gtaaatagag cgcaatatcg
    26161 gaaataacat cgccagtttc attcaaaatc gcccccagac gtgtttgctg attgcactca
    26221 cgcgccaaca tgccatccag cgcattgagc accatacgga taaaaagcac gatgggcaat
    26281 agcaaaaaga ggatgggttg tgccaccaac accaacagca atccggtaaa aagagaaagc
    26341 gccagtgccg taagagtgat gtgattcgct gtaacgtggt gcttataaag ccaaaacatc
    26401 gtcggcctta acagcgactg aaagagcggt tttatctggt agagcgtcat tatcaatcct
    26461 tgataattcg gtttgcgcta tggtgcatca attcccctga cggagatatc gcaacacgcc
    26521 gcttcattaa aaattcagaa tatatcaagg tattggggac tcactgccag gaatgattat
    26581 ccccaattaa tcaaagggtt aagaaatatt acgttgtgga tcaatacaac tcatcaagtt
    26641 ttctcatcgc ttgttcccgt attgcgcgcg tcttaccgtt aagcgccagc tggcggacta
    26701 aatccagttc agggttaaca agctctaacg ccagcaaagg atcacgtttc agcctcgcct
    26761 ttaatacatc aactggaaaa cggggattat tcatcgccac aatccaccac tcgggatcaa
    26821 tttctgccgc cagtactgca ggcggtgtgt ggcggttgtt aagtgcgccg aacttgaaaa
    26881 atgtgagatt gcttttcgcc agtgtactcc accatatacg atccggttgt tttgccagta
    26941 catccagttg tgctaacgca tcggggtcga gtgcataata gacgcgtaat gcttcatcaa
    27001 cctgcgcacg ggagctgttt tcctgcctca gaaaaaactt cgcccaaata tcattgcgtg
    27061 gcgtattttg ctgtaattcc ttgaggacaa cagcgggtaa attatcccaa ctaactaatg
    27121 cactctctaa aaagtcccag tcttccaaac gtatcagggc tgtggcattt aatagcatct
    27181 gatcatcaac ttcaccgtgg ctgataagct cagacaaaat catatcagcg ctggtttcat
    27241 acgcagctga atctgctttc ggattatttt tccgatgcga taaagcacgt tgccaaattt
    27301 tttgtttcat cgacgtgctc agatactgat ttttcgccag attactccac ccctcaggat
    27361 cgttgaaacg tgtcaataac cgttgtagag cagcgtcaga gtgaattacc tgcatatccc
    27421 gacagcaaaa gtaggcaggg gattttttct ccatcctttt aacaaaatta aactgtaagt
    27481 tatctggcaa tgctaaaaat aacacacgta ttagatcatc gtcattctcg aatttttggt
    27541 ataaataggc tgcaactttg tcactctctt gtggcgtcat ccgcaataat tgaccatggt
    27601 gtttcatctc tgccagcttg aacgctaagc ttgtgacaac attgtttgat gaatcgttcg
    27661 ccaactgcat tgcctgattg taggtgagtt gtcctccagc accgacctga cgaatagagg
    27721 aatcatcact gcggatatag ccatcaataa tggattcagg caataaccac gcatctttat
    27781 aaagctcctg aatgatcata tcccaatctc ttttcgccag agtcattacg atatccagag
    27841 ggagattttc acgtagcgca agattcaacg gaccgacacc atcaggtaga cgactcattc
    27901 gcgtcattat ttcgacagag agcggttcac ggtcgagcaa ccccagaaca acaggctggt
    27961 aacccaattg ctgatcggtc aactccagaa tctcattgtc aggcatatat ctcgccatac
    28021 tttcaatcgt ttgttcatca ttgctattaa taagttgttt gcgtaggtta gctgaaattc
    28081 ctttaagcct tgggagagtt cggattatgg caagatcaaa tgtcgcaata tctcgtcctt
    28141 caaatgcagc aacgttgcct tcatccagca tttgcaattc ctgcactaac tcaggagaag
    28201 gcttagggcg aaacagcgct tcttttttca tctgattaca gacattccct gccatgcgaa
    28261 tattgcttcg cttaaaaatt tctttcagag catcttcatc atttattaga ccccaataat
    28321 atgcatcagg atttattatc atcagcgctt ttttgacatt ttcagtgtca tttttgtcac
    28381 tcgcacggac catgaattca ctatttgtcg aagacgcaac aacaggtgca attatgtcat
    28441 tgataaatat cccacgcgca ttaatactcc ccttaatcgt agacggatta tcgtaatatg
    28501 agtcgataaa taccagtcca cttgcattta tcgaaccggt aattaacagt ggtgaatccg
    28561 taactatatt cgttgccgtc acgttaccga gaataaccca tggattagaa agagagaaag
    28621 tggaaatatt acctttgact gtcaaattac caagaacaat caatttttga ttactctcta
    28681 tattaccttc taccactaaa tcgccacgat gcactttcca gtccgatgca gataaaccga
    28741 aggggacaag attcacgcta ctgttgatag cttgttcaac ttgttgttta tcttcagcgg
    28801 tagccgcaga aaaaaatggt tcaatatcaa ctgtatttgc aacacatcga agttccgcct
    28861 gtactggaag actcagcata aacaggggaa gtaaacctat ccagccgcaa atcctggcct
    28921 taaacatatg cactccatgc cattaaaaaa gaaacaaaag ggaaatgcat ctttcccttt
    28981 tgtatattcg ttattctcgc gaaataccat ccaacgtaga caacacttcc tcagaaagat
    29041 gcaatttttc agcagccata ttctcccgta aatgcgcaac agaagacgtc cctgggatca
    29101 gcaaaatatt cggtgaacgt tgtaacagcc acgccagcgc cacctgcatg ggtgttgcac
    29161 ccaggctcgc agcaacatcc gacagcgtag aggattgcag cggtgtaaag cccccgagcg
    29221 ggaagaacgg cacgtaggca atgccgtcac gagccaaagc atcaatcatt gcgtcatcag
    29281 cacgatgagc aatgttgtat tcgttctgca cacagacgat ttcggcaatc ttgcgcgcct
    29341 ctgcaacctg cgtcggtgtg acgttgctta ggccaatatg ttttaccagg ccttgttgtt
    29401 gcatctctgc cagcacggtc aggctggctt caatcgatcc ttccgctggg ccatgaccat
    29461 cccccatcat aatgcgcaag ttaaccacat ccagtacgtc cagcccgaga ttacgtagat
    29521 tatcgtgcac cgctttttgc agctctgccg gagaaaatgc gggcaaccag gatgcgtcct
    29581 ctccacgccg cgcaccaatt ttagtgacaa ttgtcaggtc gtcagagtaa ggataaagcg
    29641 cttcgcggat aatctgattg gtgacgtgcg gaccataaaa gtcgctggta tcaatgtgat
    29701 tgacgcccaa tgccagcgcc tcgcgcagca cggttatagc gacgtggcga tctcttgggg
    29761 gaccaaaaac cccaggacct gccagttgca tcgcgccata accaagacgg ttaacggatt
    29821 ttgtaccgag agtaaatgta ttgctgctca tcgcaccctg ccctgtaata aaacggattg
    29881 agaaaaatca gtatagaaac gaaaagagat aaataatgcg aaggcgtaaa caagattact
    29941 ggatgagttg ctctattgtc ctgaaagaca ccaaaacaaa acgcgtaaag cgccgcattc
    30001 ggcaataaaa aaaccgcaac ctgtatcagc gttgcggttt aggtatcaca tgctaaaaat
    30061 tagaacgttc agtatctgga cgttactgtt tcggtcgcag attctcatca aataccaggc
    30121 gtttcctgcc aggttcaact tcccgcaatg gttcgacttg atgtaacgtc tgcttacagc
    30181 gtgtcaccag cacctgatac tcatgcgtac cctgcttttt ccatgccagc tcctgctcac
    30241 tgagttcacg aatcgctttc gccgggctgc cgacaatcag gtaattggca ggcatttctg
    30301 ctttggcttt cacaaatgcg gatgcgccaa caatgctgtt ctcgccaatc accgcgccgt
    30361 ccatcactac cgcgttcatt cccactaatg cattgcggcg gataatgcaa ccatgaagga
    30421 tagcgctatg accaatatgt ccatcttctt ccacgacagt atcctgctcg ggaaaaccgt
    30481 gcataacgca attatcctga atgttcgcgc catctttcac cacgatacga ccaaaatcgc
    30541 cacgcaggct ggcatttggc ccaacgtaaa cgcccttgcc tagaataaca tcgccgatca
    30601 ataccgctgt cggatggaca aaactctctt ctggcacaac cggagtcaga ccatctatct
    30661 ggtaaattgg catatcgcct cctgttcaat attcaaaccg ccaaaacgtt gaaaatacag
    30721 gcttcccggc gcaggcagtt caccgaccga ggtttcacct ttttcactaa cgaacgctaa
    30781 agcagcaggc gctacgcgct gataaatgtt gatacacagc tgacgcgccg tatgccctgc
    30841 ccagtgcgcc ggaagcaact cctctggcaa caatgggtct ttaaggacga cacggcgata
    30901 aaaatggatc agtaaaagct gaatatgaaa tgcacgctcc ggggttaact cgtctgctgc
    30961 ctcttttaac agcggcacca gcgggcggaa tgactgaata aaggtttcgt acatggcatt
    31021 ttgttcagtt aaatgccagc actcttccac tctggcacgc agtgctgcac gagaaagcgc
    31081 cagtggtatt tgcgcttcaa aacaaatcac gttatccgcc acccccgctt catgcaaaag
    31141 tgtctgtaca tccgccagtt tttgcgacgg cgatgccatc aggctgggtg ccagtgcgcc
    31201 aaaaccttgc cagatcaact gctttttgac atcagccagc gttgatttat ctaaaccttc
    31261 cgagagcaac aggagccatt taccatccca tgcaggttgc tctgcgcgat aaattttact
    31321 ttctgcccgt cgcgtcaggc gcaagccttt atcactgagg ctatagaaac tgcgtcgccc
    31381 gatgcgggaa acatccagcc agccttcttt attaagacga aacaaagcgg tgcgcacgaa
    31441 acgctcacca aatcccagcc cttccagcaa agcagccaga ctacccagcc agatttcacc
    31501 accacgatgg gaaagcgaat caccatacag agaggagatc aaagatgtgc cactgaccgg
    31561 aacagcgttt acagcatgtt ggataaaagt aacaagttta ctcattcgat tcagtctgtt
    31621 gttattgttg tgatttatga atcatagcac aggtcgtatc gacttctggc cctggcgctt
    31681 tacctcacca gggccagacg acacgtcagg caccaacaat attgcgcaga tcaaacaccc
    31741 gacacgcctt gccttctgaa cgcgggatac tgccacagtt aacgatcatc acatcggtag
    31801 agatccccac catcgactta atccgatggc gcagctggtg gcacacctgg caacgctgct
    31861 catgcgtcaa tgtcaggctg ctttctttca actccacttt cacagaaagt gaatcaagat
    31921 gcccccggcg gttcacctcc agttggtaat ggggcgataa atgttcgaat ttgacaatct
    31981 cttcttccag ttgtgacggg aagacattta caccgcgaat gatcaacatg tcatcgctgc
    32041 gtccgctgat gcgatccatg cggcgcatag tccgcgccgt tcctggtaac agtcgggtta
    32101 aatcacgcgt gcggtaacga atgaccggca atgcttcttt ggtcagcgtg gtgaataaca
    32161 gttcgccatg ttcaccatcg gcaagcggtg tgccgtcatg aggattaaca atttcaggat
    32221 agaaatgatc ttcccaaatg gttgggccgt cggtagtttc cagacactcc atcgccaccc
    32281 ccggccccat cacttctgac aggccataaa tatccagtgc ggtgatcccc agacgccgtt
    32341 caatctcttt acgcatggcc tgtgtccacg gctcagcacc aaacactcca acccgcagcg
    32401 agcaaccgct ggcatcaccg cccaactgcc gctccagctc ttcaatcagg ttaaggcaat
    32461 aagatggcgt aaccatgatc atatctggtt gaaaatcgcg gatcagttgc gcttgttttt
    32521 ccgtctgccc accagacatc gggatcaccg tagcgcccag acgttcggca ccatagtgcg
    32581 cgcccagccc accagtaaac aggccgtaac cataggcaac atgaattttg tctttgggcg
    32641 tgccacctgc cgcacgcaag gaacgcgcta caatattggc ccagttatca atatcgtttt
    32701 gcgtatagcc gacaactgtc ggtttacctg tggttcccga agaagcatga atgcgcacca
    32761 cttgttccat cggcacggca aaggtgtcga agggatagtt gtcgcgcaga tcctgtttgg
    32821 tggtacaggg aaatttacgc agatctgaaa gttccctgaa atcatcagga tgtacgcctg
    32881 ctgcgtcaaa tttgcgccga tacatcggga cattttcata cgcgtgtttg agcgtccatt
    32941 tcagacgttg tgtttgcaac gcctgtaact catcaacaga cgcggtttca atcgggtcaa
    33001 gctttgtatt ggttatcatt gtagggtact cgcaggttga tatgctcaaa cacgctccag
    33061 aatcatggcg atgccctgac cgacaccgat gcacatggtg cacaatgcgt aacgaccgtt
    33121 acgccgatgc agctcatggc tggcagccag tgccaggcgg gcaccactca ttcccaacgg
    33181 atggcctaag gcgatagcgc ctccgttggg gttaacatgt ggggcatcat caggcagccc
    33241 caattcgcgt agtacaccca acgcctgggc cgcgaacgct tcgttcagtt caatcacgtc
    33301 catatcgtga atactcagcc ccgcgcgttc cagcacccgg cgagttgcag gcaccggacc
    33361 aagccccatc aggcgcggtt ccaccccggc ggttgccatg gctacgattc gcgcccgcgg
    33421 tgtcagtcct tgcgctgctg ccatctgttc actggcaata atcaacgcag cggctccgtc
    33481 attcaccccg gaggcattgc ctgcggtaat caccccattg gcacgaaatg gtgcttttaa
    33541 cccacgtaac tgttccagcg tcgtttccgg gcgcagatgc tcatcatgtt gtatttctgt
    33601 tacaacgcct ttcttgtttt tcaacacaac cggaacaatc tcctcagcca gaatgcctga
    33661 ggattgcgct tttgccgtac gttgctgact gcgtagcgca aaactatctt gatcttctcg
    33721 cgatattttt aacagttcag ctacattctc tgccgtttcc ggcatgctgt cagttccaaa
    33781 ttgttgagcc atgagcgggt tcacaaatcg ccagccaata gtggtatcaa acatctcagc
    33841 ctgacgagaa aatgcactgg ttgccttgcc cataacaaac ggtgcccgag acattgactc
    33901 cacgccacca gcgatcagca aatcgccatc gcccgcttta atcgcccgtg cggcgaaccc
    33961 cagtgcgtcc agcccggaac cacacaagcg gttaatggtt gtgccggaaa cactctgcgg
    34021 cagccccgcc agtaacgtcg ccatccgggc tacgttacgg ttgtcttctc ccgcctgatt
    34081 agcacagccg aggatcacat catcgataca ctccgcatcg agacgcgggt ttcgcaccag
    34141 cagctcccgc aaagggatag cagccagatc gtcagcccga acacctgata atgccccacc
    34201 atagcggcca attggcgtac gaattccgtc acagataaag gcttcacgca tcaggcttct
    34261 cctgtaatgg tgccgccgat gcggtgagat ttaccgcgaa acagcgcaac cgttttttgt
    34321 tgttggttaa caatttcgat gtcgtaaaca ccggtttgct tgccctgatg acgtacctgc
    34381 gcagtagcag ttaaggtgtc tccggcaaag cctggacgca aaaaatcaat cgtgcaggca
    34441 gaagcgacgg ctgccagccc ctggctattg caggcgtagg caaaggcagt atcagccagt
    34501 gaaaatagct gcccgccgtg gcaactttga tgaccgttaa gcatttgtgc agtgacggtc
    34561 atggtcacta cagcaaagcc ttcatccatt gagataatgt cgatgccaag cgctttggcg
    34621 caggcatcgt tctcatacat tgcatgggca ttttgccagg ccttatgact cataaccgct
    34681 ctccagaagc gctcgttggc gcagcaatga acatgggcga tagcgttctt cgccatagtg
    34741 atgttgtaga ttttccagga gccttaatat tcgctgccag ccaagttgcg ctccccaggc
    34801 aagcgggcca tatggataat tcaccccaag acgcatggcg gtatcgatat cctgttcaga
    34861 ggccacgcct ttttgcagcg catcaagggc ttcattgatg atcattgcta ccgttcgcca
    34921 aatcagcatt cctgggtaat ctgcaatttg cagcactgtt ttgccctgct gttgcaggta
    34981 ataaatggcc ttgcgggtcg ctgagtccgg gttcaccgct gccgcagcaa tggtcaccac
    35041 cttgcccgcc attttgtcga ccactaccac cgggcgtgcc agtcgtattg ccagcgcctg
    35101 tgccgtctcg ccttgtgtct caatcaataa aacatcgtca atttccgtga cgccgtcact
    35161 tttcttttct actttcattg ggctaaaact gtcgcttacc gcttccaggc caacaactgc
    35221 ctcacgttcc gcgcgccagt cgtacacgcc cagcccactt ttcttgccca accgtccacc
    35281 aatcaccagt tcctgttgca ccagcgaagg taaaaaacga cgctcctgcc agaaagcgtt
    35341 aaacaccgaa caggtgacag caaaattgac gtcctgacca atcagatcgg ttaattccag
    35401 cggccccatc gggaaaccag cgccatcgcg aagtgcagcg tcaatcactt ctggtgcagc
    35461 aacctgctct tccagtgccc gccaggcctc ggaataataa ggacgcgcca cacggttaac
    35521 gataaatcca ggggtcgaat gacagcgcac aggctgctta ccccaactca acgttagttc
    35581 acacaactgc tcaacaactt ccgctgccgt tgccagccca ctgaccacct ccaccaactt
    35641 catcaccggt gccgggttaa aaaaatgcag ccccgcaaca cgttcaggat tttttacctc
    35701 cgcagcaatc gcggttatag agattgacga agtgttagtg gtcaatagcg tttgtggcgg
    35761 gcaaacttcc gccagctgtg caaagagcgc ctttttgact tccagacgtt cagacgccgc
    35821 ttcaatgacc aggtccgcag ctgccagcgc gtgaatatcg gtcaccggga tcaggcgttt
    35881 caatgtgcgt tcacaggttt cagcagtcag ttttccccgc gtcacgcgtg aatttagccg
    35941 cgcgtgtatc ccgtcgattg cgcgggtcag cgcttcagca gaaatgtcat acagtaaaac
    36001 ctgatgtcca tgactggcgg caacttcagc aatgccagcc cccatggtgc cgctcccaat
    36061 cactgccaca gtttgcacat ttatcatcat gctatttccc cgtgaactgc ggtgagcgtt
    36121 tagccaggaa cgcactgaca ccttcacgat aatctgcgct ccgtccggca agtcgctgat
    36181 agtcgcgttc cagatccagt tgcgtatcga gcgtattggt ttcagcgcta tttatcgctt
    36241 gcttgataag tccaagacca aatgtcggtt gtgtcgccag atgccgtgcc agctgttgcg
    36301 cggtatctgc cagcgtttca tcatcaacaa cctgccagat catcccccat tcgtgcgcct
    36361 gttcagcact cagttgattc cccagcagtg ccagccccat ggcgcgcgct cgcccagcaa
    36421 cgcgtggcag taaccaggtt ccaccgcaat cgggtattaa gcctaactta ctaaacgcca
    36481 tgacgaattt tgctgaacgg gcagcaataa cgatgtcgca ctccagtgcc agtgttgcgc
    36541 ctgcccctgc cgccacgcca ttgactgcac agatcaccgg ttttggcagt tttgccaggc
    36601 gacgtaccag tgggttatag aaacgttcaa ctgacatccc taaatccggt gcggggcctg
    36661 tgggatcaac gttacgatcg ttaagatcct gaccagcaca aaatccgcgc ccggcacctg
    36721 taagtaacag gcaacggata gtgtcgtcgc gctcgacctg tttcaggcac tctgccagtt
    36781 gtgcgtgcat ctcatcatta aaactgttca ggcgttccgg gcggttgagc gttagtgtca
    36841 tcacgccctt ttctacatga ctgaggatga attccatcat tagcgtcctt taaagtcggg
    36901 cgtgcgtttt tgtaagaaag cggagatgcc ttcatgacga tcttctgttg ccgccagcaa
    36961 ggtgaataac tgtcgctcct gggcaagtcc ggcttgcaaa gccacttcct gcgactggcg
    37021 cagcgcttgc tttgccgctt gtaaggccag cggcgagtga cgtgccattt tcgatgccag
    37081 ctgtaaggcg tattcgaggg ttaaatcgct ggggaagacg tcgctaacca gcccggcctg
    37141 ctgtgcttgc tgagcggtga tactttctcc gctcagcacc attttgctgg ctaacgattt
    37201 accgacacta cggattaaac gttgcgttcc tcctgcgcct ggcatgatgc cgagagtgat
    37261 ttccggcaaa ccaaaacgcg cgttctctcc ggcaaccacc acatcgcaca acaatgccag
    37321 ttcgcaaccc gcaccaagcg cgtaaccgtt gacggctgcg atgagaggtt tgttgaaggc
    37381 ctgcaatcgc gcccatagct gcgggcgtgt atcgtttaag gtggccgcga gatctttttc
    37441 tgccatttcg ttgagatcgg ccccagcggc aaaaaagcgt gcattaccgg taatcacaca
    37501 gaccgaaatg ctgctatcgg tagccgcagc ttccagttca tttaccagtt gcgtcagaag
    37561 ggcattattt agcgcattac gtgcggcggg acggttaagg gtcagcaaca atacttgttg
    37621 ctgacggctg acgatcagtt cgctcatgcc atccccttcg cgtcaaagtc aaccaccaca
    37681 tcgctggtca gcggcagtgc ctggcaactc aacacatagc ctgcggccag ttcatccggt
    37741 tccagactgt aattggtttc catcgccact ttgccacgca gcactttgca tttgcaggtc
    37801 gcacagacgc cgcctttgca ggcatagggc agatcagccc cctggcgcaa tgccgcatcg
    37861 agaatgcttt catcgtcggc attaagcacg atttcccgat cccgcccatc ctgacgtaca
    37921 gtcacttttt gtccgtcact ttgcacgtta acgctacgtt tgacgcgcgt gccaggcgta
    37981 ttaaaccgct ccagatgaat ggttttatct ggcattccca gtgcttttaa ggcggcttcc
    38041 gtctcatcca tcatcgccgc cggaccacaa ataaatgcct catcataaag acgaaaatta
    38101 atgagcgagg ccccaagtga ctgtaatttt tcaccgtcaa tacgcccgtg aagcagatcg
    38161 ctgtcgaggg tttcctgact gaaaatgcac aacaactgta aacgctgagg atatttgtct
    38221 ttcaggtctg ccagtgcctg gcgaaacatc atgctctggc tggtacggtt accgtagatc
    38281 agggtgaact gactttcagg ctcggtttgt aaagtggtgg cgataatcgc cagcattggc
    38341 gtaatacctg atcctgctgc aatagccaga tagcggccct ggcgttcggc ctgtggctga
    38401 tagccgaaat gcccctgcgg caccatgacc tccagcgtca taccctggcg gatgtgttcg
    38461 cgggcatagc gggagaaacg tccgccttca atggctttca ccgccacact aatttcgcca
    38521 ggcaggtagc tgcggcaaat ggagtaacaa cggcgtaatt cttcaccatc aagactggct
    38581 tttaaggtca aatgttgacc ggggcgaaag cgatacgcct cctgcaaagg ctggggcacc
    38641 gcaaaggtaa tggtcaccgc atcacgggtt tccggctcca cttttgccac cgttaaggaa
    38701 tgaaacgttg tcatggcatc ctcaaataca tttgaaataa tcgaaaggtt cgcggcaact
    38761 atcgcagcga tacaatgctt tgcaggccgt ggaaccaaat tcactgataa gtgtggtatg
    38821 gacgctggcg cagcgcgggc aacgtacttc tggcggcaaa tgggcatggc aactgtgtcc
    38881 ggcgggcggg ctaatgccat actgtcgcag acgctcacgg gcatcggggg tcatccagtc
    38941 ggtggtccat gccgggtcga gttgcagcac aacctgaacg ggggtaaacc cgtgggttgt
    39001 cattgcctca cgtatcgcgc caatcaaatg ttccgttgcc ggacaaccgg aataagtcgg
    39061 cgtaaagccg atcacccatc cttctcccat ctgtgtcaca ttacgcacca tgcctaaatc
    39121 ggtaatggtc agcaccggga tctccgggtc cgggatctgg ctgagtagcg cccatatctc
    39181 atgaacctgt ggcggtgcaa tggtggccag acgttgcata cccatctcct ctgttaccat
    39241 tgctgaccgg gcaagacacg ctggagatac tgcatttctg ccagcatggg tccaaggtgt
    39301 tcggtatgca gtcctttttt accgccagtg cgatacgcct gctcttgcgg tacgttcaat
    39361 gtggcttcgt tgatcccagc aaaaacttcg gcttcccacg ctgcgcgtaa agtgcgtgga
    39421 tcaaccgcaa taccctcttc acttagtgca atatcaatct cgtcggcatc gaacagttcg
    39481 gcggtaaaac gccacaactt gttgatggcc tgctgcatct tttgccctga tacgtcagta
    39541 ccattgccca gtcgctccag ccagccacga ctaaaacgca ggtgataacg cgcttcttta
    39601 attgctttgg cagaaatcgc cgccagttgc ggatcacggc tctccatcag acgggtaaag
    39661 agcgccacat gccatgcatc gatgaaatac tggcgtgcaa tggtgtcggc aaaattgccg
    39721 tttggctgtt caaccaacaa taagttgctg aactggcgct cgtctcgggt gaaggccagg
    39781 gtatcttcat cgccttctcc cgctaattca gcggcatacg ataagaagtt gcgtgcctga
    39841 cctaataaat cgaggccaat gtttgccagt gcgagatcga tttccagttc cggtgcgtga
    39901 ccgcaccatt cccccaaccg ctgggagagc accaggcagt tatcgcccag gcgcaaggtg
    39961 taagccgtta actgattcat ttccgacctc acatgtgctc aatgccatca gggatggtgt
    40021 aaaacgttgg atggcgatag accttgcttt cagccggatc aaaaaattca ccgcgttctt
    40081 ccggttgcga ggcaacaatt tcactcgcct tcaccaccca aattgaacat ccttcgctac
    40141 gacgggtgta agcatcacgg gcattttcca gtgccatccg ctcatcggca gcatgtaaac
    40201 tgccaacatg gcggtgtgat aagccctgtt tgccacgcac gaaaacttcg tataacggcc
    40261 agtaaacatt actcattttg aatctcctta tgcgacctta cgggcatgtt gtttttgtgc
    40321 atgggccagc gctgcttccc gtacccaggt accttcttcc caggctttac gtttggcgtc
    40381 gagcctctcc tgattacaaa ttccgcgacc gttaattact tcgttaaact cctgccagtc
    40441 gatctcacca aagcggtagt gaccgctttc agtgtcaaaa tgcagatccg ggtcaggaac
    40501 ggtcataccg agcatttcaa cctgtggaac ggtgttgtcc acgaaacgct ggcggagttc
    40561 gtcgttggtg aaacgtttga ttttccaggc gagacttctg gcgctgtttg gcgagttatc
    40621 atcgtttggc ccgaacatca ttaaggctgg ccaccagaaa cggttaatgg cgtcttgcaa
    40681 catctgcttt tgagcttcgc tgccctgcgc cagtgccatg caggcttcaa acccctgacg
    40741 ctggtgaaaa ctctcttctt tgcagatttt caccattgct ctggcatacg ggccataaga
    40801 agtgcggcat aacgccacct gattgacgat cgctgcgcca tccaccagcc agccgataac
    40861 gccgatatca gcccaactca atgtcggata attaaagatg gaggaatatt tcatccgtcc
    40921 gtcgagcatt ttttggtaga tgtcttctct ggcgcagccc agtgtttcgg cggcgctata
    40981 gagatacagg ccatgacctg cttcatcctg cactttggct aacagaatgg ctttacgccg
    41041 caaggttggt gcgcgagtga tccagttacc ttcaggcaac ataccaacaa tctcggaatg
    41101 cgcatgctgc ccaatctggc ggatcaatgt ctttcggtaa gcatcgggca tccagtcctg
    41161 tggctcgata gccgtttcct gggctatccg ttgctcaaag cgttcttctt gggtcacgtc
    41221 atcaccttta cgattccagt tttcgtgaat taattaaaaa cacgaatcac attgttttat
    41281 gaaagttaca caatggttaa ctaaaatcac aaattttgta tgcttgaact gtgacagatt
    41341 tcgcactcta ttttgataac tcattattta tcagtgcatt aatgttttta gtagttgata
    41401 tacagatcac gaagttaaca tttcgttaaa agatcccttg ctttttatga ttcgcgattt
    41461 aactattagc aacagaaatg tgaaacatct ggagagtagc gatgcagcag ttagccagtt
    41521 tcttatccgg tacctggcag tctggccggg gccgtagccg tttgattcac cacgctatta
    41581 gcggcgaggc gttatgggaa gtgaccagtg aaggtcttga tatggcggct gcccgccagt
    41641 ttgccattga aaaaggtgcc cccgccctcc gcgcgatgac ctttatcgaa cgtgcggcga
    41701 tgcttaaagc ggtcgctaaa catctgctga gtgaaaaaga gcgtttctat gctctttctg
    41761 cgcaaacagg cgcaacgcgg gcagacagtt gggttgatat tgaaggtggc attgggacgt
    41821 tatttactta cgccagcctc ggtagccggg agctgcctga cgatacgctg tggccggaag
    41881 atgaattgat ccccttatcg aaagaaggtg gatttgccgc gcgccatgta ctgacctcga
    41941 agtcaggcgt ggcagtgcat attaacgcct ttaacttccc ctgctgggga atgctggaaa
    42001 agctggcacc aacgtggctg ggcggaatgc cagccatcat caaaccagct accgcgacgg
    42061 cccaactgac tcaggcgatg gtgaaatcaa ttgtcgatag tggtcttgtt cccgaaggcg
    42121 caattagtct gatctgcggt agtgctggcg acttgttgga tcatctggac agccaggatg
    42181 tggtgacttt cacggggtca gcggcgaccg gacagatgct gcgagttcag ccaaatatcg
    42241 tcgccaaatc tatccccttc actatggaag ctgattccct gaactgctgc gtactgggcg
    42301 aagatgtcac cccggatcaa ccggagtttg cgctgtttat tcgtgaagtt gtgcgtgaga
    42361 tgaccacaaa agccgggcaa aaatgtacgg caatccggcg gattattgtg ccgcaggcat
    42421 tggttaatgc tgtcagtgat gctctggttg cgcgattaca gaaagtcgtg gtcggtgatc
    42481 ctgctcagga aggcgtgaaa atgggcgcac tggtaaatgc tgagcagcgt gccgatgtgc
    42541 aggaaaaagt gaacatattg ctggctgcag gatgcgagat tcgcctcggt ggtcaggcgg
    42601 atttatctgc tgcgggtgcc ttcttcccgc caaccttatt gtactgtccg cagccggatg
    42661 aaacaccggc ggtacatgca acagaagcct ttggccctgt cgcaacgctg atgccagcac
    42721 aaaaccagcg acatgctctg caactggctt gtgcaggcgg cggtagcctt gcgggaacgc
    42781 tggtgacggc tgatccgcaa attgcgcgtc agtttattac cgacgcggca cgtacgcatg
    42841 ggcgaattca gatcctcaat gaagagtcgg caaaagaatc caccgggcat ggctccccac
    42901 tgccacaact ggtacatggt gggcctggtc gcgcaggagg cggtgaagaa ttaggtggtt
    42961 tacgagcggt gaaacattac atgcagcgaa ccgctgttca gggtagtccg acgatgcttg
    43021 cagctatcag taaacagtgg gtgcgtggtg cgaaagtcga agaagatcgt attcatccgt
    43081 tccgcaaata ttttgaggag ctacaaccag gcgacagcct gctgactccc cgccgcacaa
    43141 tgacagaggc cgatattgtt aactttgctt gcctcagcgg cgatcatttc tatgcgcata
    43201 tggataagat tgctgctgcc gaatctattt tcggtgagcg tgtggtgcat gggtattttg
    43261 tgctttctgc ggctgcgggt ctgtttgtcg atgccggtgt cggtccggtc attgctaatt
    43321 atgggctgga aaacttgcgc tttatcgaac ccgtaaagcc aggcgatacc atccaggtgc
    43381 gtctcacctg taagcgcaag acgctgaaaa aacagcgtag cgcagaagaa aaaccaacag
    43441 gtgtggtgga atgggcggta gaggtattca atcagcatca aaccccggtg gcgctgtatt
    43501 caattctgac gctggtggcc aggcagcacg gtgattttgt cgattaatcg gtgaatgaag
    43561 ggcaacggcc aatagttgcc cttttatttc actaagtttt gtgacgttgt cacattatgc
    43621 atgatgtgta catctatttt cagggcaccc actgtatgaa aagctggcac acctgccaaa
    43681 caacctggca ggtgcaggca atcccctttg catcagtact gataatgtga acctgactaa
    43741 accgcccaca gagcgcggtt gctaacaaga acacaacatc tgacgaggtt aataatggga
    43801 agcccctctc tgtattctgc ccgtaaaaca accctggcgt tggcagtcgc cttaagtttc
    43861 gcctggcaag cgccggtatt tgcccacggt ggtgaagcgc atatggtgcc aatggatgaa
    43921 acgcttaaag aatttggtgc cgatgtgcag tgggacgact acgcccagat ctttaccctg
    43981 attaaagatg gcgcgtacgt gaaagtgaag cctggtgcgc aaacagcaat tgttaatggt
    44041 caacctctgg cactgcaagt gccggtagtg atgaaagaca ataaggcctg ggtttctgac
    44101 acctttatta acgatgtttt ccagtccggt ctggatcaaa cctttcaggt agaaaagcgc
    44161 cctcacccac ttaatgcgct aactgcggac gaaattaaac aggccgttga aattgttaaa
    44221 gcttccgcgg acttcaaacc caatacccgt tttactgaga tctccctgct accgccagat
    44281 aaagaagctg tctgggcgtt tgcgctggaa aacaaaccgg ttgaccagcc acgcaaagcc
    44341 gacgtcatta tgctcgacgg caaacatatc atcgaagcgg tggtggatct gcaaaacaac
    44401 aaactgctct cctggcaacc cattaaagac gcccacggta tggtgttgct ggatgatttc
    44461 gccagtgtgc agaacattat taacaacagt gaagaatttg ccgctgccgt gaagaaacgc
    44521 ggtattactg atgcgaaaaa agtgattacc acgccgctga ccgtaggtta tttcgatggt
    44581 aaagatggcc tgaaacaaga tgcccggttg ctcaaagtca tcagctatct tgatgtcggt
    44641 gatggcaact actgggcaca tcccatcgaa aacctggtgg cggtcgttga tttagaacag
    44701 aaaaaaatcg ttaagattga agaaggtccg gtagttccgg tgccaatgac cgcacgccca
    44761 tttgatggcc gtgaccgcgt tgctccggca gttaagccta tgcaaatcat tgagcctgaa
    44821 ggtaaaaatt acaccattac tggcgatatg attcactggc ggaactggga ttttcacctc
    44881 agcatgaact ctcgcgtcgg gccgatgatc tccaccgtga cttataacga caatggcacc
    44941 aaacgcaaag tcatgtacga aggttctctc ggcggcatga ttgtgcctta cggtgatcct
    45001 gatattggct ggtactttaa agcgtatctg gactctggtg actacggtat gggcacgcta
    45061 acctcaccaa ttgctcgtgg taaagatgcc ccgtctaacg cagtgctcct taatgaaacc
    45121 atcgccgact acactggcgt gccgatggag atccctcgcg ctatcgcggt atttgaacgt
    45181 tatgccgggc cggagtataa gcatcaggaa atgggccagc ccaacgtcag taccgaacgc
    45241 cgggagttag tggtgcgctg gatcagtaca gtgggtaact atgactacat ttttgactgg
    45301 atcttccatg aaaacggcac tattggcatt gatgccggtg ctacgggcat cgaagcggtg
    45361 aaaggtgtta aagcgaaaac catgcacgat gagacggcga aagatgacac gcgctacggc
    45421 acgcttatcg atcacaatat cgtgggtact acacaccaac atatttataa tttccgcctc
    45481 gatctggatg tagatggcga gaataacagc ctggtggcga tggacccagt ggtaaaaccg
    45541 aatactgccg gtggcccacg caccagtacc atgcaagtta atcagtacaa catcggcaat
    45601 gaacaggatg ccgcgcagaa gtttgatccg ggcacgattc gtctgttgag taacccgaac
    45661 aaagagaacc gcatgggcaa tccagtttcc tatcaaatta ttccttatgc aggtggtact
    45721 cacccggtag caaaaggtgc ccagttcgcg ccggacgagt ggatctatca tcgtttaagc
    45781 tttatggaca agcagctttg ggtaacgcgt tatcatcctg gcgagcgttt cccggaaggc
    45841 aaatatccga accgttctac tcatgacacc ggtcttggac aatacagtaa agataacgag
    45901 tcgctggaca acaccgacgc cgttgtctgg atgaccaccg gcaccacaca tgtggcccgc
    45961 gccgaagagt ggccgattat gccgaccgaa tgggtacata ctctgcttaa accatggaac
    46021 ttctttgacg aaacgccaac gctaggggcg ctgaagaaag ataagtgatt gtttcagaca
    46081 aaaaacggca ccaggtgccg ttttttactt atgagcgaac cagattaata ccgtacacac
    46141 accgacttag tttcacacca accgtccagc cagtcggggc caaaatcacg gcccgttcct
    46201 gactgcttca tcccaccaaa cggtaagtta gcgtcaatta aggtatggct gtttacccac
    46261 accgtccctg cctgtaagcg atcgctatat tccagagcct gggagagatt ttgcgtccag
    46321 acactggcag ttaagccata ttccgtgtcg tttgccagtt gtaacgcctc ttctccatcc
    46381 gctactcgca ccaggtttac caccggacca aacacctctt cacgagttaa gcgcaattta
    46441 gcatcgggat ttaccaccag cgttggcgca acataatacc cctctccggc tggtccattc
    46501 gacccgcgaa tcagctctgc ttgctgtgcc tgcgcatcgt cgaggaatga acacactttg
    46561 tcgcagtgcg cacgagaaac caaagggtta atctgtgcaa caggtgacat ccccggtccc
    46621 acttgcaacg attttaccgc ctgctcaaat ccactaacca gcgtgtcaaa caacggcgct
    46681 tcaatataaa ttcgcgaact ggcggcgcat acttgccctt gattcaggaa gcttccggtc
    46741 atcaagcctt caataaccca ttgcggatca gcatctttta atacaattgc cgggttttta
    46801 ccgcccagtt ccagcgttac acgcgttaag tgatcagcag cagttctggc aatacctttt
    46861 cccgtcgcgg ttgaaccggt aaaactgatt ttcgcaacat gaggatgtga cgtcagggcc
    46921 gcgccgcata cagcacctga cccggtgacg acattaaaaa cgccatcagg gataccagcc
    46981 tcgctggcca gttccgccac gcgcaacatc gtcagtggcg tggtttccga aggcttaatc
    47041 acgattgaac agcctgctgc cagtgctggc atcaccttcc acataccaat catcaacgga
    47101 aagttccatg gcacaattcc cgccactacg ccaaccggct ctttacgcgt ccaggcctga
    47161 taacgcgccc cctggggtaa gggaatcgac aagtccagcg ttttacccgc gattttggtc
    47221 gttaacccgg cggtataacg catccagttc agcgtacagc ccacttcaaa agcacgggaa
    47281 atggcaattg acttgccttg ctccagggtt tccagttgcg ccagctcctc actgtgctgt
    47341 tccaccagat cggcaaaacg tagcagaata cgttcacgct ctgcgggtaa tcgcccggcc
    47401 cagcgacgcg agacaaaggc ccgccaggca gacatgactg cgttatctac atccgcttcg
    47461 ttggcatcag cagtagacgc aatttcttgc ccggtggccg gatcaaagat cgccaatcgt
    47521 ttttcacttt gtgcggggcc tggacgacca tcaatataaa gaccgtgttg acgatcgaga
    47581 aactgttgga cctggcttaa tactgctaca tgcggctctg tcatcacttt tccttattat
    47641 ttacccagtg tgatttcagt gtacgcagcg acaagaaaag tcactttttt cgctgtgaca
    47701 ttcgctttgt cagctgtgac aagctccgca aatcgtgaca ataaccgctt atgtctctat
    47761 gcctgagaag acttatttcc tatagtcagg ctcattagct tcgtttactg caacacaaat
    47821 gcaacaataa aaatacattt cacagagcga aaacgtgcca tgaaccccgc agtggataat
    47881 gagtttcagc aatggctttc ccaaatcaat caggtatgcg gaaattttac cggacacctg
    47941 ctgactgagc gttacacggg tgtactggat acgcattttg ccaaaggact aaagctgagt
    48001 accgtgacaa ccagcggggt gaatttatcc cgcacctggc aggaagtaaa aggcagcgac
    48061 gatgcctggt tttacaccgt ttttcagctt agtggtcagg caataatgga gcaggatgag
    48121 cgtcaggtgc agattggcgc tggcgatatt acgttactcg atgcctcacg cccctgttcg
    48181 ctttactggc aggagtcttc taaacagatt tcattacttt tgccacgcac tctgctggaa
    48241 caatattttc cccatcaaaa acctgtctgc gcagaaagac tggacgctga cttacccatg
    48301 gtgcaactca gtcatcgcct gttacaggag agcatgaata atccggcact ttctgaaaca
    48361 gaaagtgaag ctgcgctaca ggcgatggtg tgtctgctgc gcccggtact tcatcagcgg
    48421 gaatctgttc aacctcgtcg tgaacgtcag tttcaaaaag tggttacgtt gatagacgat
    48481 aatattcgcg aagagatatt acgcccggag tggatagccg gagagacagg tatgtcagta
    48541 cgtagtttgt accgaatgtt tgccgataaa ggtttggtag tcgcgcaata tattcgtaac
    48601 cgtcgtctcg atttttgtgc agatgcgatt cgccatgccg cagatgatga aaaactggca
    48661 ggcatcggct ttcattgggg attttctgac cagagtcatt tttcaacggt atttaagcaa
    48721 cgctttggga tgacgccagg cgagtatcga cgtaaattcc gctaactaat tggcgttgtg
    48781 tgtactgcaa cgccaattag ttaaaacaga tatttgacta tgcatttttc agggcgaaag
    48841 gcaatttgac aggagttttt gaatattcac cttgtttcag gaaaatagat caatcgcttt
    48901 ctcctgtagc gttatcccga tattgcggtt ttgctattaa aattttcgca cccatttacc
    48961 gtttatccct tgtacatatt cccccggttt ggcccgagct acgagctttt gcccggcgag
    49021 tttcgcgata tcatctacag acacattatt ttgctttgcc agttgttggt aactcgcttt
    49081 acgttcggca ttaatgtcgg ttactaattt ctctgtttca gcatccgttt tcagcgcaac
    49141 cagataacca taaaatgttt cacctacccg cccctgagtt ctggcttcat ccagagtcaa
    49201 tgccattaca ttgctgctta ccagcccaac aagaaacgca caaaaaagta atgttttctt
    49261 catcatcgcc tcagaaaaga tcgctacgag tttcaagcag ctcttcgaca tctttgtctg
    49321 ccttgatgat gatctcatgc tcaattttaa cgttcatatt gatagtgatc ggttccttgg
    49381 gcgcagcgac ttcaatacgc ggcgtacagg cagccagcat aaaagatgac gtcaacgcag
    49441 ccagtaaaat tttcattgtt tttcctcaca ctcttttcct tgcggacagt catttcccag
    49501 cagacgtgcg ttctgctcca accatgcctg gagattatcg ccaaagcgta aactgcgcca
    49561 cagcgtaaaa atattctctt catgatagta attaaggttt accgtaccgc ttttaccatc
    49621 aacgcgactg gtacctgtaa tgttggcctg catggttaat aaaccgagat tatctaagtt
    49681 aatttttgtc gatgaccggc taatttccat atagcgcaac cagttaattg ctgaacccgc
    49741 agtcatattg tctttcacca ccgcatctgc ggtgtcttta tccagccgca aggtcattgg
    49801 cccgctattt gccagccaac catctttcac tatccatttt tcgttgttta gccataacgg
    49861 caatgcacca ctaaatgccc cggacatggc gaattgtttc ggattgacgg cgctaaccag
    49921 ttcgctggat gagagattat tcagccgcaa cagcgccggg tcatgttgcg gcatacgtaa
    49981 ttgtttcatc agtacgttac cgcctaacac atcgacgcta acatcagtca acagcaaggg
    50041 ttcttcttca gtccacggat aacgcccttg caaatcagcc gtaatatttt tcgctgtcac
    50101 cagattaatc acttcggcaa ttcgcaacgt aacggggccg cgagtcccca gatgccaggc
    50161 tccatcggca aaacggaaag gcaggacaaa atcgacaccg ttaacctgat tatctggcat
    50221 ccaggcacta ccgcctttca acacgccgtg tcctcccgcg cggaatcctt gttcaggcgc
    50281 agcagaaaat gcaacctgag catacagttc accatcgcgt aagttcatct tccagtcggg
    50341 tggcaccagc ggctggaata cagtcagtga ttgttttggc caccaggcgt taccgcgcag
    50401 acgaataccg tcccagcgac catttacccg aaccgggcca atctcaccag catgtaaatc
    50461 gcctttaaag aggaaatacg tcggatcgcg cccatcgacg ctaaatttta aggttgatgg
    50521 cggtaacaca ctgccgccag tgaacagcgt ttgcccggcg tccagtgaca gcgcgccgct
    50581 gaaggaggga tgctgtgcgt cacgtaccca gacgacgggc ttgtcgagaa ttaatcgcgg
    50641 cttttctacc gtcatcgtac cgtattgtaa ctggtcgaaa ccggtggaga gatcggtcag
    50701 cgtaatcgtg ctgtcatgcc actcaccttt tcctgcgaca tcccaggtgg cattcatcgg
    50761 tgtaaaactg ccttttcccc agtagcgcca ctgccagcgg ccagcgtcag ggagaaaatc
    50821 attcgccagc ccatccatat gcagcacgaa atcgcccagt tcattttcat gcgcctgcaa
    50881 aatggcctgc aaacgtccgt caacaccacg ttgggtgact tttacacccg ctaaaggcca
    50941 gcggatttca tcgatgtcca gcgaatcgat gactcttccc ttcgaacgta ataacgcgcc
    51001 gggctcaaag gccagcgttg ggtcagtcag acttccactt agctgcgcgg gtaagcgggc
    51061 atataaaatg aggtccgcct gtttcgcttc accggtcagt tgcagtggca tctgactttt
    51121 ttccatgctt aattttcctg gaccaaaatt aagcaccgcg ttgcctttac ccgcttgccc
    51181 ctgggtcagc acactcagtc gcccgctgac cagagcgttc tcgagccctg cctgccagtt
    51241 gtcgactttg acacccagtc ggccactcaa aggaaaacct gcatacggcc agctccagcg
    51301 accatcgctt acggtcaatt gttgacgagt aatttgccac ggcaaatcga gcaacggatc
    51361 gccgttatcc cgtgccagca caatcaattg cccgctattt tcctgccagt ccagctctgc
    51421 atccaccagt gacggttcct gcggcaagtt taacgtcgca gtagcatgcc cacttacagg
    51481 aagtccatcc ggcacgagcg gcatagtaaa ttcccccacc agtttaaccg gcggctgatt
    51541 ttcaaacgcg acgacatcca gttcgctgac tgtaagttgt tgccctttca gctggccttg
    51601 aaatttaact ttttcgccct gataacgcag ttgctggata tcagaggtta atgcgagaga
    51661 gagttttccc tgccattcct gccagggaga aaaaatcagt ttatcgatat tgatccaggt
    51721 gttaggcagc atggactgcc actgcgcgag ggtttttgga gcagctggcg attgctccgt
    51781 ctgcggcaat ttcgccagac aagcagaatc aagttctacc atgccgacgt tcaataacca
    51841 gcggctggga tgtgaaaggc tggcgttggt gatatgcgca agctgacaat ctcccaccag
    51901 ataacggaga tcgggaatga ttaaaccttt acgcgtaatg cgtgggcttt catctaatgc
    51961 aatacgtgtc ccgagcggta gccagatgcc cgccagcgtg ggaacccaca gcccgagcgt
    52021 catcagcagc gtcaacggca caagaataat cagtaataac agcgcgagaa cggctttata
    52081 tttacccagc atgggtagtt aatatcctga tttagcgaaa aattaagcat tcaatacggg
    52141 tattgtggca tgtttaaccg ttcagttgaa ggttgcgcct acactaagca tagttgttga
    52201 tgaatttttc aatatcgcca tagatttcaa ttatatttga aattttgtaa aatattttta
    52261 gtagcttaaa tgtgattcaa catcactgga gaaagtctta tgaaactcgc cgtttatagc
    52321 acaaaacagt acgacaagaa gtacctgcaa caggtgaacg agtcctttgg ctttgagctg
    52381 gaattttttg actttctgct gacggaaaaa accgctaaaa ctgccaatgg ctgcgaagcg
    52441 gtatgtattt tcgtaaacga tgacggcagc cgcccggtgc tggaagagct gaaaaagcac
    52501 ggcgttaaat atatcgccct gcgctgtgcc ggtttcaata acgtcgacct tgacgcggca
    52561 aaagaactgg ggctgaaagt agtccgtgtt ccagcctatg atccagaggc cgttgctgaa
    52621 cacgccatcg gtatgatgat gacgctgaac cgccgtattc accgcgcgta tcagcgtacc
    52681 cgtgacgcta acttctctct ggaaggtctg accggcttta ctatgtatgg caaaacggca
    52741 ggcgttatcg gtaccggtaa aatcggtgtg gcgatgctgc gcattctgaa aggttttggt
    52801 atgcgtctgc tggcgttcga tccgtatcca agtgcggcgg cgctggaact cggtgtggag
    52861 tatgtcgatc tgccaaccct gttctctgaa tcagacgtta tctctctgca ctgcccgctg
    52921 acaccggaaa actaccatct gttgaacgaa gccgccttcg atcagatgaa aaatggcgtg
    52981 atgatcgtca ataccagtcg cggtgcattg attgattctc aggcggcaat tgaagcgctg
    53041 aaaaatcaga aaattggttc gttgggtatg gacgtatatg agaacgaacg cgatctgttc
    53101 tttgaagata aatccaacga cgtaattcag gatgacgtat tccgtcgcct gtctgcctgc
    53161 cacaacgtgc tgtttaccgg gcatcaggca ttcctgacag cagaagctct gaccagtatt
    53221 tctcagacta cgctgcaaaa cttaagcaat ctggaaaaag gcgaaacctg cccgaacgaa
    53281 ctggtttaat cttgccgctc ccctgcattc caggggagct tattcagata atccccaaag
    53341 acctttcatc ctctattctt aaaatagccc tgagtcagaa actgtaattg agaaccacaa
    53401 tgaagaaagt agccgcgctt gttgcgctaa gcctgctgat ggcgggatgt gtaagtagtg
    53461 acaaaattgc tgtaacgcca gaacagttac agcatcatcg ctttgtgctg gaaagcgtaa
    53521 acggtaagcc cgtgaccagc gataaaaatc cgccagaaat cagctttggt gaaaaaatga
    53581 tgatttccgg cagcatgtgt aaccgcttta gcggtgaagg caaactgtct aatggtgaac
    53641 tgacagccaa agggctggca atgacccgta tgatgtgcgc taacccgcag cttaatgaac
    53701 tcgataacac cattagcgaa atgctgaaag aaggtgcaca agtggatctg accgcgaacc
    53761 agttaacgct ggcgaccgcg aaacagacat taacttataa gctggcggat ttaatgaatt
    53821 aatagctgcc acagctcccg gcggcaagtg actgttcact acagcgtttg ccgttgggta
    53881 atgcacacat cccaatcgcc gtaccatcca gttgacgggc aacagaaagc gaaccgccga
    53941 tcattgcaca atttgcttct ccactactgg acatcgacgc ttttaaacct ggcgctacgt
    54001 gcgccgcagt cgcctgctga acaggctcac tactacacgc cgacaacaat aaagcggcac
    54061 accctaccca aaacgctgct cgcatctttt cttcctctga tcttcaagcc aaacgacacc
    54121 gccataaata ataggcagca cagagggcgg cgtcgagagc tgtcctgcgc gttgccccgc
    54181 atttttactt ttttatggct atttttttgc cctctgtttg atcaaaacat tcattacgct
    54241 gatgtggggg acacaaaagc gaaaatgcag aagaaagcca tttgctaaaa ttgaaagatt
    54301 actactgggc gcgcagcaat ttcgtgcgcc cctcatttgc gcaatgtaag ggtgtcatat
    54361 gattactatt gacggtaatg gcgcggttgc ttcggtcgca tttcgcacca gtgaagttat
    54421 cgccatctac cctattaccc ccagttccac gatggcagaa caggctgatg cctgggccgg
    54481 aaacggctta aagaacgttt ggggagacac accacgcgtg gttgaaatgc agtcggaagc
    54541 gggtgctatc gctaccgtgc atggcgcttt gcagacgggt gccctttcaa catcgtttac
    54601 gtcatcgcag ggtttgctgc tgatgatccc gacgctgtac aaactggcag gcgaactaac
    54661 accgtttgtc ctgcatgtag cggcacgtac cgttgccaca catgcactct ctatttttgg
    54721 cgatcattcc gacgttatgg cggtgcgcca gacgggttgc gcgatgttgt gtgcagcaaa
    54781 cgtccaggaa gcgcaagact ttgctctcat ttcgcacatc gcgacgctga aaagccgcgt
    54841 gccatttatt catttctttg atggtttccg cacgtcccac gaaatcaata aaattgtccc
    54901 gctggccgat gacacgattc ttgatctcat gccgcaggtc gaaattgatg ctcatcgcgc
    54961 ccgggcactc aacccggaac atccggtgat ccgcggtacg tccgccaatc ctgacactta
    55021 tttccagtct cgcgaagcca ccaacccatg gtacaacgcg gtctatgacc atgttgaaca
    55081 ggcgatgaat gatttctctg ccgcgacagg tcgtcagtat cagccgtttg aatattacgg
    55141 gcatccgcaa gcggaacggg tgattatcct gatgggctct gccattggca cctgtgaaga
    55201 agtggttgat gaattgctaa cccgtggcga aaaagttggc gtgctgaaag ttcgcctgta
    55261 ccgccccttc tccgctaaac atttactgca agctctgccg ggatccgtac gcagcgtggc
    55321 ggtactggac agaaccaaag aacccggtgc ccaggcagaa ccgctctatc tggatgtaat
    55381 gaccgcactg gcagaagcct ttaataatgg cgagcgcgaa actctgcccc gtgtcattgg
    55441 tgggcgctat ggtctttcat ccaaagaatt tggcccagac tgtgtactgg cggtatttgc
    55501 cgagctcaac gcggctaaac cgaaagcgcg ctttacggtt ggtatttacg atgatgtgac
    55561 caatctgtca ctgccgttgc cggaaaacac cctgccaaac tcggcgaaac tggaagcctt
    55621 gttttatggc cttggtagtg atggcagcgt ttccgcgacc aaaaacaata tcaagattat
    55681 cggtaattcc acgccgtggt acgcacaggg ctattttgtt tacgactcca aaaaggcggg
    55741 cggcctgacg gtttctcacc ttcgagtgag cgaacagccg attcgttccg cttatctcat
    55801 ttcccaggct gattttgttg gctgccacca gttgcagttt atcgataaat atcagatggc
    55861 tgagcgttta aaacctggcg gcattttcct gctcaacacg ccgtacagcg cagatgaagt
    55921 gtggtcgcgc ttgccgcaag aagttcaggc cgtgttaaac cagaaaaaag cgcgcttcta
    55981 tgtgattaac gcggcgaaaa tcgcccgcga atgtggcctg gcggcccgta ttaataccgt
    56041 catgcagatg gcttttttcc atctgacgca aattctgcct ggcgatagcg ccctcgcaga
    56101 attgcagggt gcgattgcca aaagttacag tagcaaaggc caggatctgg tggaacgcaa
    56161 ctggcaggct ctggcgctgg cgcgtgaatc cgtagaagaa gttccgttgc aaccggtaaa
    56221 tccgcacagc gccaatcgac cgccagtggt ttccgatgcc gcccctgatt tcgtgaaaac
    56281 cgtaaccgct gcgatgctcg ccgggcttgg tgacgccctc cccgtttcgg cgctgccgcc
    56341 agacggcacc tggccgatgg gcactacgcg ctgggaaaaa cgcaatatcg ccgaagagat
    56401 ccccatctgg aaagaggaac tctgtaccca atgtaaccac tgcgttgccg cttgcccaca
    56461 ctcagctatt cgcgcaaaag tggtgccgcc tgaagcgatg gaaaacgccc ctgccagcct
    56521 gcattcgctg gatgtgaaat cgcgtgatat gcgcgggcag aaatatgtct tgcaggtggc
    56581 accggaagat tgcaccggtt gtaacctgtg cgtcgaagtt tgcccggcga aagaccgtca
    56641 gaatccagag attaaagcca tcaatatgat gtctcgcctg gaacatgtcg aagaagagaa
    56701 aatcaattac gatttcttcc tcaacctgcc agaaatcgac cgtagcaaac tggaacgtat
    56761 tgatattcgt acatcgcagc tgattacacc gctgtttgaa tattcaggtg cttgctccgg
    56821 ttgtggcgag acgccgtata ttaaattact gactcagctc tatggcgacc ggatgttgat
    56881 cgctaacgcc actggctgtt cttcaattta tggcggtaac ctgccctcta caccgtatac
    56941 caccgatgcc aacggtcgtg ggccggcatg ggcgaactct ctatttgaag ataatgccga
    57001 atttggcctt ggtttccgcc tgacggtcga tcaacaccgt gtccgcgtgc tgcgtctgct
    57061 ggatcaattt gccgataaaa tcccggcgga attactgacg gcgttgaaat cagacgccac
    57121 gccagaggtt cgtcgtgaac aggttgcagc tttacgccag caactcaacg atgttgccga
    57181 agcacatgaa ctgctacgtg atgcagatgc actggtggaa aaatcaatct ggctgattgg
    57241 tggtgatggc tgggcttacg atatcggctt tggcggtctg gatcatgtat tgagtttgac
    57301 ggaaaacgtc aacattctgg tgctggatac gcaatgctat tccaacaccg gtggtcaggc
    57361 atcgaaagcg acaccgctgg gtgcagtaac taaatttggc gagcacggca aacgtaaagc
    57421 gcgtaaagat cttggcgtca gtatgatgat gtacggtcat gtttatgtgg cgcagatttc
    57481 tctcggcgcg cagctgaacc agacggtgaa agcgattcag gaagcggaag cgtatccggg
    57541 gccatcgctg atcattgctt atagcccgtg tgaagagcat ggttacgatc tggcactcag
    57601 ccacgaccag atgcgccaac tcacagctac cggcttctgg ccgctatatc gctttgatcc
    57661 gcgtcgtgcc gatgaaggca aactgccgct ggccttggat tcacgcccgc cgtcagaagc
    57721 actggaagaa acgttacttc acgagcaacg tttccgtcgg ctgaattcgc agcagccaga
    57781 agtggcagaa cagttatgga aagatgctgc agctgatttg caaaaacgct atgacttcct
    57841 ggcacaaatg gccggaaaag cggaaaaaag caacaccgat taaatgctct ggataaggat
    57901 tatccaattc taaaaaaaaa gcccggacga ctgttcgggc ttgtcttttt atatgttgga
    57961 aaatcagtgg caatgcaatg gcccaacaga aaataaaatc tgtgacaata aaggcatata
    58021 acccgcgcag aataacgtat agcaaattaa aagtataatt tttattttgt atgtattatc
    58081 ttcgatacat tcaccagact tattatcatt tcagaggaat tatttgatta aggttttact
    58141 taaggcgtaa caaatgatat ttatcggcta actgaacttc tcctttagga tgttttcact
    58201 ccccgaaatg gggataaaag gcaaataaaa taactaaagg atttattcaa tgaaaagcaa
    58261 agtactggca cttttaattc ctgccctgct cgccgcaggt gctgcacatg cagccgaagt
    58321 ttataataaa gacggcaaca aattagatct gtatggcaaa gttgatggcc tgcattattt
    58381 ttctgataat tcagcgaaag atggcgacca gagctatgcg cgtctgggtt ttaaaggcga
    58441 aacccaaatt aacgatcaac tcactggcta cggtcaatgg gaatacaata ttcaggcaaa
    58501 caacactgaa tcttcaaaaa accagtcatg gacccgtctg gcatttgccg ggctgaaatt
    58561 tgcagattac ggttctttcg attacggacg taattatggc gtaatgtacg acatcgaagg
    58621 ctggaccgat atgctgcctg aatttggcgg tgactcttat accaatgcag acaactttat
    58681 gactggtcga gccaatggcg tcgcgactta tcgtaatact gatttcttcg gtctggtaaa
    58741 tggtctgaac ttcgcggtgc agtatcaagg taacaacgaa ggagccagta atggtcagga
    58801 aggcaccaac aacggacgtg atgttcgcca tgaaaacggt gacggctggg gtctttccac
    58861 aacatatgat ttaggcatgg gctttagcgc tggtgcggca tacacctctt ctgaccgcac
    58921 caatgaccag gttaaccata ctgcggcggg tggtgataaa gcagacgcgt ggactgctgg
    58981 gctaaaatac gatgctaaca atatttacct ggcaaccatg tattcagaaa cgcgtaatat
    59041 gaccccgttt ggcgacagcg attatgctgt tgcaaacaaa acccagaact ttgaagtcac
    59101 tgcacagtac cagtttgatt ttggtctgcg tccggcagtc tctttcctga tgtctaaagg
    59161 ccgtgacctg cacgctgccg gtggtgcaga caacccggca ggtgttgatg ataaagatct
    59221 ggttaaatac gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacttatgt
    59281 tgactataaa atcaacctgt tggatgaaga tgacagtttc tacgctgcca atggcatctc
    59341 taccgatgat attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt
    59401 tatggcgggc tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc
    59461 gtcattacac aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt
    59521 tttatgaaca gaacgattct tgtccctatc gatatttccg attcagaatt aactcaacgc
    59581 gtgattagcc acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg
    59641 gtaatacctt cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg
    59701 gcaatggatg acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt
    59761 aaactgccaa ccgacagagt gcatgtccat gttgaggaag gctcgccaaa agaccgcatt
    59821 ctggaattgg cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat
    59881 atcaccactt atctgctcgg ttctaacgcc gcagctgtag tgcgtcacgc agagtgctcc
    59941 gtgctggttg tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc
    60001 gcaaatgtgc tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca
    60061 gatttcttat gctgggcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt
    60121 gagtcgcttt taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa
    60181 caaattacaa aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat
    60241 tgaagaaggc gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct
    60301 ggagattctg cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa
    60361 cctcgatcaa aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct
    60421 gggcgttgag tacaagattg ttgaagagaa tacttacggt atcgtgaaag agaagattcc
    60481 agagggcaaa accacttgct cactgtgttc tcgccttcgt cgcggtatcc tttatcgtac
    60541 cgcaacggaa ctgggggcga cgaagatcgc gttgggtcac catcgtgacg atatcctgca
    60601 aacgttgttc ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat
    60661 gagcgatgat ggcaaacata tcgttattcg tccgctggcc tactgccgcg agaaagatat
    60721 tcagcgattt gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca
    60781 gcctaacctg caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc
    60841 agggcgtatc gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga
    60901 tactaacctg ttcgatttca aaggcatcac ccacggttct gaagtggtta acgggggtga
    60961 tctggcgttt gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga
    61021 tgaaaatcag ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caagatggcg
    61081 cccgatgcgc aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc
    61141 gcatccggca atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta
    61201 ttttaataac cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca
    61261 tgctttatga gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat
    61321 tttgccaata tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc
    61381 cgggcgcatt ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag
    61441 cggcacaatg gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc
    61501 agaaatgata ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct
    61561 atttcctgca cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag
    61621 ctcaaagttc accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt
    61681 cgcaaccagt acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgatcgcg
    61741 ttgctccaaa tcgccgtgta atgacaatgc actttgccct acttcattca gcgcgtcgca
    61801 gacagcctgg caatcttttt tggtattgca aaacaccacg caagaggatg gctgatgcaa
    61861 gcttaataac cgttgcaaca gaggaatttt gcctttgctg gatgtctcat aaaattgttg
    61921 ttcaatgggt ggcaaagcat ctgttgagtc aatttcaatc gccaaaggat cgcgttgcac
    61981 tcgtccgctg attacggcga tggcttccgg ccaggttgcc gaaaacagaa gcgtctgtcg
    62041 agatgcaggc gcaaaacgga tgacatcatc aatggcatcg ctaaatccca tatccagcat
    62101 gcggtcggcc tcatccatca ccaaagtatt cagcgcatcc agtgataccg tgcctttttg
    62161 caagtgatcc agcaaacgac ccggcgttgc cacgataata tgcggcgcat gttgcaacga
    62221 atcacgctgc ataccgaacg gttgaccacc gcacaacgtc aaaattttgg tatttgggag
    62281 aaaacgcgcc agccgacgca attcacctgc cacctgatcc gccagttcac gcgtaggaca
    62341 cagcactaaa gcctgggttt gaaatagcga cgcatcaatt tgctgtaaca agccgaggcc
    62401 aaaagccgcc gttttgccgc tgccggtttt cgcctgcacg cgaacatctt ttccggcaag
    62461 gatcgccgga agcgcggcga cctgcaccgg cgtcatggtt aaataaccca actcattaag
    62521 gttcgtgagt tgggcgggag gcaaaacatt cagggtagaa aaagcggtca caatctattc
    62581 tcgtggtcat cgacgcaaag ttagcaggcg cgtatcctcg cagatctacg ctcacgatgc
    62641 gacaatttaa tcggttcttc atcaggtggt gggtcaggca tgggttgcgg gcgagggatc
    62701 ggatcgggca ctggaacagg atcgccagga atcggttcag ggacaggaat ttgcaaataa
    62761 ataagtgtcg tcatatttcc ctccggtcat tgggtggact cttaaagggt agacgctgat
    62821 aaataacagg caaaaaaaag ccgactcatc aaagtcggcg tcgtacgaat caattgtgct
    62881 atgcagtaat tcaaaaaagg aagtaagaca atatggagcg caacgcccat cgcttgacgt
    62941 tgcattcacc tgcaagggag atattgccct gaatgggtag agagtttatt gacttcgctc
    63001 aaactttgcg gcgtttttgt atacaaacag ccggaaaaat tgcttttgtt acaaccattt
    63061 actacgatgc aaccataaag caacaccacc aataagaaca actaacagaa tacaaaaaat
    63121 tgaaaatccg aattgccacc cgccgccagg gatcccacca aggttgacgc caaataaccc
    63181 tgtcagaaag gtactgggta aaaagaccat tgccatcaat gacattgtat aggtacgacg
    63241 agctaaattt tcctgcatca cctgagcgat ttcatccgcc atcacgccag tccgtgctat
    63301 acaggcgtcg atttcgtcaa gaccgcgccc aaggcgatcg gcaatatcct gcatccgacg
    63361 gcgttggtca tcgctcatcc acggcaaacg ttcactggca agacgagcat aaacatcacg
    63421 ttgcggtgcc atatagcgac gcatcacgat taattgtttg cgcagcagag ccaggaatcc
    63481 acgcggtgga atttgctgat caaggagatt atcttcaagg tcgataattt tatcgtgcag
    63541 ctgctcgata aattcgctgg aatgatcggt caacgcatcg cacacatcca ccagccaccc
    63601 tccgcaatcg gtcggacccg tgccctcttc cagatcgctc accacatcgt ccagcgccag
    63661 cactttgcgt tgtcgggtcg aaacaattaa ccgcccgtcc atatatacac gcatggcgac
    63721 cagttgatcg gggcgttcat cggtgctgcc gtttatacag cgcaatgtaa tcagcgtgcc
    63781 ttcaccgaga cggctgactc ggggccgagt gctctcgcct gccagcgcat cacgtacgtt
    63841 attgggaagc agcggtgttg tcgccagcca ttgggcgctt tcatggtgta cataattaag
    63901 gtggagccag cagggatgcg cttcatcaat cacatctgta ttttccagcg gtttaacgcc
    63961 gcctctacca tccagcatcc aggcaaatac tgcatccggg acattaacgt ccgatccctt
    64021 aatcgcttcc acagtgcctc catcatcaac gcattatttt gtagtctagc cttctggccc
    64081 tgttacgcaa catctcatca ccccattacc ctgaaatgat taataaaatt ctgtctaaat
    64141 tgaatacaaa aagcaaaatg cttttccgta tacaaaccgt gtgaagtgtt aaatagcgtc
    64201 tatcattatc agaattatct gatcatatga cgtggctttt ttgcgatcgg atagcaacaa
    64261 aaattgataa aaataacggg atctcaatga ttacgcacaa cttcaatacc ttggacttac
    64321 tcaccagtcc tgtctggatt gtttcgccct ttgaggaaca gttaatttat gccaatagcg
    64381 cggcgcgact gttgatgcaa gacctcacgt ttagtcagct acgaaccgga ccctattccg
    64441 tctcctcaca aaaagaactg ccgaaatacc tctccgatct gcaaaaccaa cacgatatta
    64501 tcgaaatcct cactgttcag cgtaaagaag aggaaacagc attaagctgt cggcttgttt
    64561 tgcgagagct gacagaaaca gaaccggtga ttattttcga aggtatcgaa gcgccggcaa
    64621 cgctgggttt aaaagccagt cgctcggcaa attatcagcg caaaaaacaa ggtttttatg
    64681 cgcgcttttt tctgactaac tctgcaccaa tgttgttgat tgacccctca cgagatggcc
    64741 aaatcgtcga tgctaacctc gccgcgctca atttctatgg ttataaccat gaaacgatgt
    64801 gccagaaaca tacctgggaa attaatatgc tcgggcgtcg cgtcatgcct atcatgcatg
    64861 aaatttcgca tttacccggc ggtcataaac ctttgaattt tgttcataaa ctggcggatg
    64921 gatctactcg tcatgtgcag acctatgccg ggccgattga aatttatggc gacaagctca
    64981 tgttatgtat tgtgcatgat attactgagc aaaaactgct ggaggagcag ctggaacatg
    65041 ctgctcacca tgacgcgatg accggattac tgaatcggcg acagttttat cacattaccg
    65101 agccaggcca aatgcagcat ctcgccatcg ctcaggatta cagcttgttg ctcatcgaca
    65161 ccgatcgttt taaacacatt aacgatctct atgggcattc taaaggtgat gaggtgttat
    65221 gcgccctcgc ccgcaccctt gaaagttgcg ctcgcaaagg cgatttggtg tttcgttggg
    65281 gaggcgaaga gtttgtctta ttgctaccaa gaaccccact ggataccgcg ctttcgctgg
    65341 ctgaaactat ccgtgtaagc gtggcaaaag tgagtatttc gggcttacca cgctttaccg
    65401 tcagcattgg tgtggcgcat cacgaaggaa atgaaagcat cgatgaactg tttaaacgcg
    65461 ttgatgatgc tttgtatcgg gcgaaaaatg atggacgcaa ccgcgtgctg gcggcataag
    65521 ccgcggatgc gtctcgagat caacgactgc gcttagcgtg gcgctcccag ttttcttgct
    65581 tcgcctgcgc cgttttacgt agtgcgacgt aacacgcccc gctgccgcca tgatgcggta
    65641 gcgcagtgca atatgcctga acatcatcaa attcggtcag ccagcgcgcc acatagctgc
    65701 ggacaatatt ggcatgcgat ttatcatccc gtcctttgcc atgaataatc agcacgttac
    65761 gcaaaccatc agccatggct tgttgaatga aactgaacat cattttgcgg cactcttcca
    65821 ccggctggcg caaaagattc aggctcgcct gttgcggata tttaccactg cgcagcttat
    65881 ccagcacccc atgttgcaac ccttcccgcc gaaactccag cggctgactt agtgggatga
    65941 tgtcgagaaa tccggtggtg aggaaattat caagctgcag cgtgtcgata cgctgcggcg
    66001 cacgttggtt acgcgttgga tgccagtgga catcggtagc acgtttcagc ggctggacat
    66061 cttccatggc gtcaagaaac agcgatttgt cgtcaaggtt catgttacat cctcccgcaa
    66121 ttaagagcgc gatatgataa ccagaccggg tcggtccaac aacgtattac ccaaaattcc
    66181 agtaataagt tccaaatatt gccgatattt taagcaaaat gcttatgcat gattattcat
    66241 tcacgatatt aataatgtaa cttatatttt cgtgaaatct gtcactgaag aaaattggca
    66301 actaaaggtt aaaaccgtta taacacagtc accggcgcag aggagacaat gccggactta
    66361 agacgcggat gcactgctgt gtgtactgta gagtctggcg gatgtcgaca gactctattt
    66421 ttttatgcag ttttaacttt gcagatagcc gcattctcgc cggaattgcg tgattaatga
    66481 cgcggtcagt ggtgtctggc gactatcacg ccgctgaatc aaataatagg ccgctttcgg
    66541 taaaatttcg ctaaccggca acatcaccag cccctgtccg tgcaagggat cgcagcccat
    66601 ttcttcaggc agtatgctga gaaaatcgct ttttgccacc agactgatac aggctgagaa
    66661 cgtctcgcag actacaccga cctgtggcgt ttgcgcctga tcgtcaagca attcactcaa
    66721 ctgtttgtag taactgccgt gtggcgtcgg cattgtccag ctgtaatcca gtaactgttt
    66781 gatcgaacgg gcaccaatgg cggggtgtcc cgggcggcaa aagatcgcga attgcttttc
    66841 cagtaatttc tcaaaagtaa attcgtggtc gtacggtccc tgataatagg tattgatggt
    66901 gaaatccaat tctccctgac gcaattcatt aatcatcgac accagttgcc cttccataat
    66961 gcgtactttt acctgcggat gctgctgatg aaaacgagat atgacagctg gcatcagact
    67021 gcgggaaata ctggccccca tgccgatatt aatctgccct gccagttgcc cttgtcgttg
    67081 gcgaatttcc tcttgcgctg cgcgcagctc ttcaagaata agactggcgt gctgataaaa
    67141 actttcaccg gcatcagtta acgtcacgcc tttactacgg cgaaaaaaga gttgtgccgc
    67201 taatccttct tccagctcct gaatagattt actcagtgcc ggttgcgaca tattcaacat
    67261 tcggctcgct ccgcgaatgc tgccctgacg agccacttca acaaaagcac gaatttgatg
    67321 aatttttacc tgaaaagcca tgacgccacc gataaccgtt atttatcaga ccaaagaaac
    67381 tggcatctac tttaatgcag atgattgtgt cagggtaatt tatgaacggt taaaactgtg
    67441 aaaaatcagt tagtgataag taaaaactat cgctacgtga accgggtcac actttttact
    67501 gatgacggga aaggttatgg agtctttgaa tcaatttgtt aattcgcttg ccccaaaatt
    67561 atcgcactgg cgacgtgatt ttcatcacta tgcagagtct ggctgggtgg aattccgcac
    67621 tgccaccctt gttgcggaag aattgcagca gctcggctat tcactggcgc tgggccgcga
    67681 agtagttaat gaaagtagcc ggatgggact acctgatgaa ctcactctac aacgcgaatt
    67741 cgagcgcgct cgtcaacagg gtgcgctagc acaatggatt gcggtttttg aaggtggttt
    67801 cactggtatc gtcgccaccc tggataccgg tcgccccggt ccggtgatgg ctttccgtgt
    67861 cgatatggac gcgctggatc tcagtgaaga gcaggatgtc agccatcgcc cctaccgcga
    67921 cggttttgcg tcatgtaacg ccggaatgat gcatgcctgt ggtcatgatg gacataccgc
    67981 cattgggctt gggctggcgc atacccttaa acaattcgag tccggactac atggcgtcat
    68041 caaactgatt tttcagcctg cagaggaagg tacgcgtggc gcgcgggcga tggtcgatgc
    68101 aggtgtcgta gatgatgttg attattttac tgccgtgcac attggcactg gcgtacctgc
    68161 gggcaccgtg gtgtgcggca gtgataattt tatggcaacc accaaatttg acgcgcactt
    68221 caccggtacc gccgctcacg caggcgcaaa accagaagac ggtcacaatg ccttgttggc
    68281 ggcagcacaa gccactcttg cactgcatgc aatcgccccg cacagcgaag gagcttccag
    68341 agtaaacgtg ggcgttatgc aggcaggaag cggtcgtaac gttgttcctg cctcggcgtt
    68401 gctgaaagtg gaaacacgcg gggccagcga cgtcattaat caatatgttt ttgaccgtgc
    68461 acaacaagcg attcagggcg cagcaaccat gtatggtgtc ggcgttgaaa ctcgtctgat
    68521 gggtgcagct accgccagtt ctccttcgcc gcaatgggtc gcatggttgc aaagtcaggc
    68581 ggctcaggtc gcgggggtca atcaggccat tgaacgtgtt gaagcgcctg cgggttccga
    68641 agatgccaca ttaatgatgg cccgcgtgca gcaacatcaa gggcaagcct cctacgtggt
    68701 gtttggcaca cagctggcgg caggtcatca caacgaaaaa ttcgattttg acgagcaggt
    68761 tctcgctatt gccgtcgaaa cgctggcgcg caccgcgctc aattttccct ggacgcgagg
    68821 tatctgatgc aggaaatcta tcgttttatc gacgatgcga ttgaagccga tcgccaacgt
    68881 tataccgata ttgccgatca aatctgggat catccagaaa cacgttttga agagttctgg
    68941 tcagcggagc atctggcttc ggcgctggaa tctgcaagct tcaccgttac ccgcaacgta
    69001 ggcaatatcc caaatgcatt tattgcttcg tttggtcaag gcaaaccggt tatcgccctg
    69061 ctgggagaat atgacgccct ggcaggttta agtcagcaag ctggttgcgc gcaacctaca
    69121 tccgcgacgc ccggtgaaaa tggtcacggt tgcggacaca atttgctggg aaccgccgcc
    69181 tttgccgctg caatagccgt caagaaatgg ctggaacaat atgggcaagg cggcacagtg
    69241 cgcttttatg gttgtcctgg cgaagaaggc ggctcgggta aaacgttcat ggtccgcgag
    69301 ggggtatttg atgatgtgga tgcggcactc acctggcacc cggaagcctt tgccggcatg
    69361 ttcaataccc gcacgctggc aaacattcag gcatcatggt gctttaaagg gatcgcagca
    69421 catgccgcga attcccctca tttgggacgc agcgcccttg atgccgtaac gttgatgacc
    69481 actggcacca acttcctcaa cgaacatatt attgaaaaag cgcgcgtaca ctatgccatc
    69541 acaaatagcg gcgggatctc gcccaacgtg gtccaggcgc aggcagaagt gctttatctt
    69601 atccgtgccc ccgaaatgac cgacgtgcag catatttatg atcgggtcgc caaaatcgcc
    69661 gaaggtgcgg cattgatgac cgaaaccacg gttgaatgcc gcttcgacaa agcctgttcc
    69721 agttatctcc cgaatcgcac cttagaaaat gccatgtacc atgccctatc ccattttggt
    69781 accccggaat ggaactccga agaactggct tttgcgaaac aaattcaggc tacgctcacc
    69841 cccaacgatc ggcaaaacag tctgaataat atcgccgcaa ccggtggcga aaacggcaag
    69901 gtttttgcac tacgtcatcg tgaaacggta ctggcgaatg aggtcgctcc atatgccgcc
    69961 accgataacg tgcttgcggc atcgactgat gtcggcgacg tcagttggaa actgcctgtt
    70021 gcccagtgtt tcagcccctg ttttgccgtc ggtacaccgc tacatacgtg gcaactggtt
    70081 agccaggggc gaacatctat tgctcataaa ggaatgctgc tggcggcgaa aactatggca
    70141 gcaaccacag tcaatctctt cattgattca gggctattgc aagaatgcca acaagagcat
    70201 cagcaagtta cggacacgca accgtatcac tgccctatcc cgaaaaacgt gacaccgtca
    70261 cctttaaaat aacaacaacg caaacacaac aaccgaggaa tgcccatgag tatgtcatcc
    70321 ataccgtcgt cctcccaatc cgggaagctc tatggctggg tcgaaagaat tggtaacaag
    70381 gttccccatc cttttttgct ctttatctat ttgataatcg tactcatggt gacgacggca
    70441 attttgtcag cctttggcgt cagtgcgaaa aacccgaccg atggtacgcc ggtggtggtg
    70501 aaaaacctgc tcagtgtgga aggattacac tggtttttac ccaatgtgat taaaaacttt
    70561 agcggttttg ctccacttgg agcgatcctg gcgctggttt taggtgccgg cctagcggag
    70621 cgcgtcggct tactgccagc gctaatggtt aaaatggcat cgcatgttaa tgcccgctac
    70681 gccagttata tggtgctgtt tattgctttt ttcagccaca tttcttccga tgcggcgtta
    70741 gtgatcatgc caccgatggg tgcgctgatt tttctggcgg tgggcaggca tccagttgca
    70801 ggtttactgg ctgccattgc aggcgtaggt tgcggcttta cggctaattt actgattgtc
    70861 acaaccgacg tgttgctgtc ggggatcagc acggaagcgg cggctgcgtt caatccgcaa
    70921 atgcacgtca gtgtaattga taactggtat tttatggcca gctccgtagt cgtactgacg
    70981 attgttggcg gcttgataac cgacaaaatc atcgagccac ggttaggtca atggcaggga
    71041 aacagcgatg agaaactgca gacattgacc gaaagtcagc gttttggttt acgcatagca
    71101 ggtgtcgtat cgctactttt tattgctgcg atcgcgctga tggtgatccc ggaaaacggg
    71161 atattgcgcg atccgattaa tcacaccgtg atgccatcac cctttattaa aggtatcgtg
    71221 ccactaatca ttcttttttt ctttgtggtt tcgctggctt atggcatcgc tacccgcaca
    71281 attcgacgtc aggcggattt accgcattta atgattgaac cgatgaaaga gatggcggga
    71341 tttatcgtga tggtttttcc cctcgcccag tttgtcgcca tgtttaactg gagcaacatg
    71401 gggaaattca tcgccgtggg gctgaccgat atacttgaaa gttcagggct tagcggcatc
    71461 ccggcgtttg tcggtctggc gttgctttcc tctttcttat gcatgtttat tgccagcggt
    71521 tccgcaatct ggtcgattct ggcccccatt ttcgtaccaa tgtttatgct acttggcttt
    71581 cacccggcat ttgcgcaaat cctctttcgt attgccgact catccgtatt gcctttagcg
    71641 ccggtatctc cttttgttcc actgtttctt ggattcctgc aacgctacaa accagacgcg
    71701 aaactgggta cttactattc gttagtcttg ccctatccgc ttatcttttt ggtggtatgg
    71761 ctgctgatgt tgctggcgtg gtatcttgtt ggtctgccga taggtccggg gatttaccca
    71821 cgtttgtctt aagagagaac ggatgctgag attacttgaa gaaaaaattg ccacgccact
    71881 gggtccactg tgggtgattt gcgatgagca atttcgcctg cgggcggttg aatgggaaga
    71941 gtacagcgaa cgcatggtgc agctgctgga catccattat cgcaaagaag gctatgagcg
    72001 catttctgcc accaacccag gcggtttaag cgacaagctt cgtgattatt ttgccggtaa
    72061 tcttagcatt attgatacgc ttcccactgc cacagggggg acgccatttc agcgcgaagt
    72121 ctggaaaact ctacgcacta tcccctgcgg gcaggtaatg cattacggcc aactggctga
    72181 acaattgggc cgccctggcg cggcgcgtgc cgttggtgcg gcaaacggat cgaatcccat
    72241 cagcatcgtc gtaccttgcc atcgggttat tggccgaaac ggcaccatga ccggatatgc
    72301 aggcggagtt cagcgaaaag agtggttatt gcgccacgaa ggttatcttt tgctgtaaac
    72361 attaaacaat ttgtgccagc ttgttcacac ttttatgtaa agttaccctt aacaacttaa
    72421 gggttttcaa atagatagac atatatttac atctaatatc ggaattctct gctgttaagg
    72481 tttgcttaga cttacttgct ccctaaaaag atgttaaaat tgacaaatat caattacggc
    72541 ttgagcagac ctatgatccc ggaaaagcga attatacggc gcattcagtc tggcggttgt
    72601 gctatccatt gccaggattg cagcatcagc cagctttgca tcccgttcac actcaacgaa
    72661 catgagcttg atcagcttga taatatcatt gagcggaaga agcctattca gaaaggccag
    72721 acgctgttta aggctggtga tgaacttaaa tcgctttatg ccatccgctc cggtacgatt
    72781 aaaagttata ccatcactga gcaaggcgac gagcaaatca ctggtttcca tttagcaggc
    72841 gacctggtgg gatttgacgc catcggcagc ggccatcacc cgagcttcgc gcaggcgctg
    72901 gaaacctcga tggtatgtga aatcccgttc gaaacgctgg acgatttgtc tggtaaaatg
    72961 ccgaatctgc gtcagcagat gatgcgtctg atgagcggtg aaatcaaagg cgatcaggac
    73021 atgatcctgc tgttgtcgaa gaaaaatgcc gaggaacgtc tggctgcatt catctacaac
    73081 ctgtcccgtc gttttgccca acgcggcttc tcccctcgtg aattccgcct gacgatgact
    73141 cgtggcgata tcggtaacta tctgggcctg acggtagaaa ccatcagccg tctgctgggt
    73201 cgcttccaga aaagcggtat gctggcagtc aaaggtaaat acatcaccat cgaaaataac
    73261 gatgcgctgg cccagcttgc tggtcatacg cgtaacgttg cctgattttt ccgcataact
    73321 cactatcctt ctgtcatatc attaaatttt tctgatttat tgatctggca gaaggttcat
    73381 cactgtttca ttcaccagat atgggttaat cttttaatta caaactgcgt tgacagttgt
    73441 tgtaaggaga ccctgtatgg ctatgtatca gaacatgctc gttgttatcg atcctaacca
    73501 ggacgaccaa ccagcattgc ggcgagctgt ttatttacat caacggattg gtggcaaaat
    73561 taaagccttt ttgccgatct atgacttctc atacgaaatg accaccctgc tctccccgga
    73621 cgaacgtacc gctatgcgtc agggcgtcat cagccagcgt acagcctgga tccacgagca
    73681 ggcaaaatat tatctcaatg ctggcgttcc cattgaaatt aaagtggtct ggcataaccg
    73741 tcccttcgaa gccatcattc aggaagtgat cagcggcggg cacgatttgg tgctaaaaat
    73801 ggcgcaccaa catgaccgtc tggaagcggt gatttttacg ccaacggact ggcatctgtt
    73861 acgcaaatgc ccaagcccgg tgtggatggt gaaagaccag ccgtggccgg aaggaggcaa
    73921 ggcgctggtg gcggtgaatc tcgccagtga agagccgtat cataatgcgc tcaatgaaaa
    73981 actggtcaaa gagacgatcg aactggcaga acaagtcaac cataccgaag ttcatctggt
    74041 tggcgcttat ccggtaacgc caattaatat cgcgattgaa ctgccggaat ttgatccgag
    74101 cgtttataac gatgccattc gtgggcaaca tttgctggca atgaaagctc tgcggcagaa
    74161 attcggcatt aatgaaaaca tgacgcacgt agaaaaaggt ctgccagaag aggtgattcc
    74221 tgatttggcg gagcacttac aggcggggat tgtggttctg ggcacggtag gacgcaccgg
    74281 tatttcagca gcattcctcg gcaacacggc ggaacaggtg attgatcatc ttcgctgcga
    74341 tctgttggtc attaaacctg accagtatca gacacccgtt gaactggatg acgaagaaga
    74401 cgattaacga ctcttcaata actgacaagg cccgagttat cgggcctatt ttttacttac
    74461 tgctcttcgc cgccaaggaa ataaattccc aacgggattg ccagcaacac agtaaagacc
    74521 aggctataaa caaaaatcat cgatgactga atcacataca ttgatgtcgt ccactcagac
    74581 agcggaatat tgtactgttc tataacgccg ccgatagtcg cgcgagtcac aaccgacgcc
    74641 gcaataatga aaaccgccaa caaaccaaag agaaatttct tccctttcgc tgacttcagt
    74701 ttcgccataa tcatgatgta atccttgtgc agacaaattt cttgttacct aaacgtaaca
    74761 ataccatgac atttcaccgt tgtctgctct gaaaacctca cctcttagct ggatttacaa
    74821 tcaacgcatt gattagttag ctaatgttgc tacttccttc cctgactatt gatgatctga
    74881 atgaattgtc gcggtaaaat agccccgcca ggggtttccg gcatgacgac aattatctgg
    74941 aggagcctga taaaacaatg atcgctgaac tgtttacaaa taatgcgctt aatctggtca
    75001 ttattttcgg tagctgcgca gcattgattc tgatgagctt ttggtttcgc cgcggaaatc
    75061 gtaaaagaaa aggtttttta ttccatgcgg tgcaattttt aatctacacc ataattatca
    75121 gtgctgttgg tagcattatt aattatgtta tagaaaacta taaactcaaa tttatcaccc
    75181 caggcgttat cgattttatc tgtacgtccc tgattgcggt tattctgacg attaagctat
    75241 ttttgctgat taatcagttt gaaaaacagc agattaaaaa aggtcgcgat atcaccagtg
    75301 cgcggattat gtcgcgcatt atcaaaatca ccattattgt ggtgcttgtt ctgctttatg
    75361 gcgaacattt cggcatgagc ctttctggct tgctgacctt tggtggtatt ggtggtctgg
    75421 ctgtcggtat ggccggtaaa gatattctga gtaatttctt ttccgggatc atgctctatt
    75481 tcgaccgtcc tttcagtatt ggcgactgga tccgttcacc ggacagaaat atcgaaggta
    75541 cagtagcgga aattggctgg cgaattacca aaattacgac ctttgataat cgtccattgt
    75601 acgtaccgaa ctcgctgttt tcgtcgatca gcgtagaaaa cccaggacga atgaccaacc
    75661 gccgcattac cacgaccatt ggtttacgtt atgaggatgc ggcaaaagtg ggcgttattg
    75721 tcgaagccgt acgtgagatg ctgaaaaatc acccggccat cgaccagcga caaaccttac
    75781 tggtttattt caaccagttt gccgactcgt cattgaatat tatggtttat tgctttacca
    75841 aaaccacggt atgggctgaa tggcttgctg cacagcaaga cgtttatttg aagattatcg
    75901 atattgtgca gtcacacggc gcggactttg ccttccccag ccagacgctg tatatggata
    75961 acattacacc gcaggaacag gggcgctaaa tcagacatca ctggcgttat ccacaagggt
    76021 aacgccagtt cccatcacat caatgcttca caatatacat agtccggctg tacgccacat
    76081 cttccgggtt attaatggga taacctttca gccacggctt gattaatcgt ccattggtat
    76141 attgataaat tggtgcaatc ggtgcttgct ccatgaggat tttttctgcc gcgttgtaat
    76201 cggcattacg cgctttaacg gtattttccg tcgatgcctg ggccagaact ttgtcgtatg
    76261 ccggattgtt aaagcgtgaa atatttcctg aatgcgttga cgttaataat gtcaggaaag
    76321 tggagggttc attataatcc cccacccatg aggcgcggat aacatcaaaa ttgccggtgt
    76381 tacggctatc gatataggtt ttccattcct gattttgcaa tttaacatct acgccaaggt
    76441 tctttttcca catcgatgct acagcaatcg caattttttg atggttttct gaagtgttat
    76501 acaaaagcgt cagcttcagc ggtttttgcg gaccataacc agctgcgctc aacaaagttt
    76561 ttgcctgcgc attcagttct tcctgactca tttgttcaaa cggcgaaggt tccggcgtaa
    76621 atccggcggt aacatctggt gtaaaatgcc acgctggctt ttcgcccgtc cctaatactt
    76681 tttcggtcat caggcggcga tctatcgtca tacttaatgc cagacgaacg cgctgatctg
    76741 ccgtcggccc tttttgcgtg ttaaacgcat aataataggt cccgagctgc ggcggcgtat
    76801 aaacctgccc cggaatatcc ttcaacagct tctgatacat atttttcggg aaggattcgg
    76861 tgatatcaat atcccccgcg aggtaacgct tagtggctgc ggattcctga ttaattggca
    76921 ggaaggtcac tttttgcagt accgttttgg cgttatccca ataatgggta ttcggtacca
    76981 cgaccagttt ttcattgact acgcgatctt taagaacata agcgccattg ccgatcagat
    77041 ttccgggttt cgtccactct ttaccgcttt ctacgttggc tttttgcacc gggaagaagg
    77101 caaagttagc ggttaaattc acaaaccacg gcaacggttt atcaagctga attttcaagg
    77161 tatgggcatc aactgcggtg acgccaagct ggtcaggcgt agctttacca tcaataatcg
    77221 cctgtgcgtt gttgattccc gccagcgcgg caaaccatgc aaatggcgat agcgttttcg
    77281 ggtccaccag acgctgccag ctgtagacaa aatcttgcgc cgttaccggt gtgccatccg
    77341 cccattttgc gttatcgcgc agggtaaaag tccagatacg gttgtcatta cttttccact
    77401 gagtcgcaac gccggggaca atctcccctt tctcgttctg attcaccagt ccttcaaaca
    77461 gatcgcgaat gacctgaatc tctggcaggc ccacggcttt agcgggatcc agcgacgcag
    77521 gctcatcttt aatgtggcgc accagctcct gcttctctgc cagtactgtg ccgctcggaa
    77581 cttctgcagc atacgaaaga gaaatgctgc tgaccaacag cgcacaacac gtgactgaaa
    77641 cagagtgctt cataagtttc cctctaaccg acattaaaat tgatgcgtaa ttatttgttt
    77701 cgccttaaag aaatgcaaac aacttccatc aaaaaagtga cacaggaagc ataaaatgtt
    77761 ttatgtatca atatattaaa tcagatttag tgtaaataat cgacgccaaa atgcggtaaa
    77821 gcacatagaa caccgaacac tacgttcagg cttcgcacgt tcaaaccagt tttcggtatc
    77881 ataatcagca tctgtcgcca ttgtacgaag aataaaaaat aaccaaaacg ttgaaaggaa
    77941 ctgagaatac gttaagagag aaattaaatt aatgtatcag caagtagtgc taaacagcac
    78001 ctttatactt cagcctatct ataaataatg agaatgccga gccagcatgt cgacggtgtg
    78061 gatagtacag gtgataaccg ggtaaatctg gtgtgaattt atccaaaaca cgtatcagtt
    78121 ttttttcttt aatagcccgc tcaacctgat cataaggtag atacgccaat ccatgcccat
    78181 caattgcagc atcaatgatc aggtctatcg tattcagtaa aagctgacct tccatgcgaa
    78241 cacgaacttc acgtccaccg cgtattaatc tccagcgatt tgctgtaccc gatgtgggaa
    78301 gatacaaatt aattgcctga tgatctatta attgtgacac tgacgttgga acacttcggc
    78361 gagaaaaata atccggtgag ccaacaatag ccattggtat atctggcccg attcgaatgg
    78421 cgatcatatc tttatccatc tccccaccca gacggacgcc tgcatcaaaa cgttcagaaa
    78481 cgacatcggt caaaccataa tcaatggtga gctgaatatc aatttcagga tgcgatttca
    78541 ggaatgtgcg cattgctggt aacaatatcg tttttgctgc atgttctaca gtagtaatac
    78601 gtattgtccc ggatgggcgg ttctgcagat cgctcaggga tgccatggct gaatctatgt
    78661 catgcaacat agggccaaga acagacaaaa gatgctcgcc cgcttcagtt ggaacaacgc
    78721 tgcgcgtggt tcgcgtcaga agccgcaatc ccaatcgttc ttctatacga cgcactatct
    78781 ggcttaaagc tgactgcgcc atgctcaggc gggctgctgc acgagtgaag ctacgctcct
    78841 ctgcaacgac gacaaacgcc atcagatcag cgatttcttc acgcttcatt cagtatctcc
    78901 atgcaatcta ttaatcattt aaatgatagg tctaatcatg attttgggtc taatcaactt
    78961 aatgatcaat atctatagtg atatcaccaa ccgttaaaac gaaccgacaa ctccatgagg
    79021 aacggaatga tgaataataa agtcagcttc actaacagca ataatccaac catctctttg
    79081 tctgcagtga tctatttccc tccaaaattt gatgaaaccc gtcagtatca ggcaattgtg
    79141 ttatctcacc caggtggggg cgttaaagaa caaacggccg gaacctatgc caaaaaactg
    79201 gcggaaaagg gatttgtcac tattgcttat gacgcatctt atcaaggtga aagtggcggc
    79261 gagccgcgtc agttagaaaa cccatacatt cgtactgaag acatcagtgc cgtgattgat
    79321 tacctgacaa cgctttctta tgtcgacaat acccgaattg gtgcgatggg aatttgtgcc
    79381 ggtgcaggat atacagccaa tgctgcaatt caggatcgcc gcatcaaggc catcggtacc
    79441 gttagtgcgg tcaatattgg ttcgatgttc cgtaatggtt gggaaaacaa tgtgaaatct
    79501 attgatgccc tgccatacgt tgaagcgggt tcaaacgccc gaaccagcga tattagcagc
    79561 ggagaatatg ccgttatgcc actggcacca atgaaggagt ccgatgcgcc gaacgaagaa
    79621 ctgcgtcagg cctgggagta ttaccacacc cctcgcgcgc agtatccaac agcaccgggt
    79681 tacgctactt tgcgcagcct taaccagatt attacctatg atgcttacca tatggcggaa
    79741 gtgtacctga ctcagccgat gcagattgtg gcgggcagcc aggcagggag caaatggatg
    79801 agtgacgacc tgtacgatcg ggcatcaagc caggataaac gctatcacat tgttgaaggc
    79861 gcaaaccata tggatcttta tgacggcaaa gcctatgtgg ctgaagcaat ttcagtatta
    79921 gctccgttct ttgaggaaac tctgtaatga aacatattca aatccgcaat agtgatatgg
    79981 cctggcatat tgctgccaat attcagtttc caccaaattt tgatgagtca aaacagtatc
    80041 cggcgattat cagcgtccat ccttttggca gttgtaagga gcagacgtct ggcaatatct
    80101 atggcaaagc attggctgaa aaaggctatc tcgttctcgc ttatgacgcc agtttccagg
    80161 gagaatctgg tggggagcct cgctggatag aagatccgac acagcgcgta gaggatatta
    80221 gccgtgttat cgattatgca gtaactctgc catatgttaa tgctgaacgt attggcgtgc
    80281 tgggagtgtg tgggggtggt ggctatgcca ttaatgccgc gctgacagaa aaacgaatta
    80341 aagcagtagt gagtattacg ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca
    80401 attacgatcc gattggcgca ttgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg
    80461 gtggagaatt acagatcaac gaattacttc ccgccagcct ggatgcagca aaagcccatg
    80521 gactgaccga acgcgatgtt tatgaagcaa ctgactacta taaaacacca cgcgggcaac
    80581 aaccgggagg tgcgacaaaa atgttgttct cacatgcaca gaaaacgctg gcctgggatg
    80641 ccttcgcatt tactgaagtg ttactgactc agccggtgat ggtagtagtt ggtgaaaaag
    80701 tcggtgcctt cggtgcttat cgtgatggac tggaagtcta tggccgggcg atggtttcgc
    80761 aggatcgtca gttagtttca ttacccgatt tctcacatta tgaactgtat gacaaaccgg
    80821 aagcagttca ggaggcgctg gaaaaagtta ttcctttttt caacacccat ctgggataag
    80881 tttcatgaga aatattatga aaaatgtact gattcttggt gctggcggtc agatagcccg
    80941 ccatgtgatt aatcaactgg cagataaaca aaccattaaa caaactttat ttgcccgtca
    81001 gccagcaaaa atccacaaac cctatccaac taatagcaag attattatgg gagatgtact
    81061 gaatcatgca gcactgaagc aggccatgca gggacaggac gttgtgtatg ccaaccttac
    81121 gggggaagat ttagatattc aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt
    81181 taaacgtctg atctttgttc tttccctcgg tatttatgat gaagttccag gaaaatttgg
    81241 cgaatggaat aatgcagtta ttggtgagcc cctaaaacca ttccgcaggg ctgcggatgc
    81301 aattgaggct tctggactgg aatatacaat tcttcgccct gcctggctga ctgatgaaga
    81361 tattattgat tatgagctta cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg
    81421 taagagtgtc gcagcattaa tcacagatat catcgataaa cctgagaaac acattggtga
    81481 taatataggt atcaatcaac ccgtgactga tggtgacaag ccattattca tgtaaatcaa
    81541 ttacatagtt accatcgtaa tgtactatgt gcagtacgat ttattaaact tattaaggtg
    81601 aactgatcaa tccgatgcga ttaacatgtg tagaaccagt aagaataaaa tacgccatgt
    81661 atataacatt acgacttaca tctaagtcag cataattcta atatctgcac atcaataaaa
    81721 atgcaaacaa cttccggcaa aaaagtgata caagatcgat gacatagcgt tgaagaaaag
    81781 gatttgataa tccgcaaaat ttgcacaaca ctgcctgtaa tacttcctga accagagacg
    81841 atcatgaccg taaaccgccc acgcgccgaa cgcggcgcat ttccgcccgg aacagaacat
    81901 tacggacgtt cattattggg cgcgccgtta atctggtttc ctgcccctgc agccagtcgc
    81961 gaaagtggtt tgattctggc tggcactcac ggtgatgaaa actcttcggt cgtcacgctc
    82021 tcctgtgctc tgcggacttt gacaccttct ttgcgccgcc atcatgtggt gctgtgtgtg
    82081 aatcctgacg gctgccagct ggggttacgg gccaatgcta atggtgtgga tttaaacaga
    82141 aactttccgg cggcgaactg gaaggaaggt gaaacggttt atcgctggaa cagcgccgct
    82201 gaagaacgtg atgtcgtatt actgacaggt gacaaacctg gctcagaacc tgaaacccag
    82261 gcgctgtgcc agcttataca ccgcattcaa cctgcctggg tggtctcatt ccacgatcca
    82321 ctggcctgta ttgaagatcc cagacacagc gaattaggcg aatggctggc ccaggcgttt
    82381 gaattgcctc tggtaaccag cgtcggttat gaaacgcctg gctctttcgg cagttggtgt
    82441 gcagatctca acttgcattg catcaccgca gagtttccgc ccatctcctc cgacgaagcc
    82501 agcgaaaaat atctctttgc gatggcaaac ttgctgcgct ggcatcctaa agatgcaatt
    82561 cgcccgtcgt gaactgaagc gccggttcca catctaccgc cagccaggtc ggtccgtcaa
    82621 gatcggcgaa actgacctgc ggcaccagcg gtaaagcggc gctaatggca cgagaggtac
    82681 acaacatgca gcccagcatc agactgaaac cttgtgcacg cgcttcagtc gccagcgcca
    82741 gcgcttccgt cagaccaccg gttttatcga gcttaatgtt aaccatctca tagcgccctt
    82801 tcagcgcctt cagattgcta cgtgtatgac aactctcatc agcacaaatc ggcaacggat
    82861 gaataaaatt ctccagcgcc gcatcgtcct gcgcaggaag gggttgttca agcatcgcaa
    82921 cgcctaaatc cgccaatagc tggcaacgcg ccgccaaccc ttctgcacgc caggattcat
    82981 ttgcatcaac gatcagcgtc gcatcgggca cagctgtgcg aattgccacc atccgctcac
    83041 taataagatg gttatccagc ttcactttca gtaatttcgc gcctgcctgc cagagtgttg
    83101 atgcactatt ggccatctga tcaggcgtac cgatgactac cgtctgtgca gtgataactg
    83161 tctcgggaag cgttatgccg atcaaatcag ccagcgattg ctgctgtttt cgcgcggcca
    83221 gatcccacaa tgcacaatcc agcgcattac gtgctgcgcc ggcagggaga attttttgca
    83281 actcctcccg tgtcagccct ttctctaatt gcgacacgac gctcataatt tgcgccatta
    83341 ccgaggcatc actttctcca taacgcggat acggcgtgca ttcgccggtg cctttaatac
    83401 cctcttcttc cagttcaacc accaccacgc gcgcttcact gcgacttccc cgggcaatca
    83461 caaacggggt atgtaagggc caggcttcct caaatacctt aacggttctc atctttactc
    83521 cttgttgtcg cggtggtctg cggattaagc gcaaatagcg tttgctgtgt tattgacagt
    83581 tagcataaac taggtgtgac gttaactata tgtaaacagg aaagataatc atgtcacaaa
    83641 ccgttcattt ccagggcaac ccggttacag tcgccaattc catcccgcag gcgggtagca
    83701 aagcgcagac ttttactctc gtagcaaaag atctgtctga cgtcaccctc ggtcagtttg
    83761 cgggtaaacg caaagtgctg aacattttcc cgagtattga taccggagtt tgcgccgcat
    83821 cagtacgtaa gtttaaccaa ctggcaaccg agatcgacaa caccgttgtg ctgtgtattt
    83881 ctgccgatct gccgttcgcc cagtctcgtt tctgcggcgc agaaggtctg aacaacgtta
    83941 tcaccctctc cactttccgt aacgctgaat tcctgcaagc ctacggtgtg gcaattgctg
    84001 atggcccact gaaaggtctg gcagcacgtg ccgttgtggt tattgacgaa aatgacaatg
    84061 tgattttcag ccagctggtg gatgaaatca ccaccgagcc ggattacgaa gcagctctgg
    84121 ctgtactgaa agcataattt aatatgcctg atggtgttgc accatcaggc atattcgcgc
    84181 ttactcttcg ttcttcttct gactcagacc atattcccgc aacttattgg caatcgcggt
    84241 atgtgaaacg ccgagacgtt ttgccagttt gcgcgtgctg ggataattgc gataaagctg
    84301 ggttaatacc gagcgttcaa aacggctggt gatttcgtcc agcgaacctt ccatcgcatc
    84361 ttcgcccacg gctaccgttg cggcgtcata atccggcaac aaaatatcct gtggacgcag
    84421 ctcataaccg tccagttgtg tcagtgcgcg atagatagcg ttctttaact gccgcacatt
    84481 tcccggccac gcataacgcg taagtacagt attcaggtca gcggccagtt tcggacgcgg
    84541 cacgccctgc tcgtcggcaa agcgggcgac gaacagctca gttaacggca tgatgtcctg
    84601 cggacagtca cgtagcggcg gcagattgag cgtcaacacg ttcagacgat aatagagatc
    84661 ttcacggaac atgccttttt gcaccagttc gaccagattc ttctgcgtag cgcaaatcac
    84721 ccgcacatcg acatgcacct catggtcttc gccaacccga cggaaagtgc catcattaag
    84781 gaaacgcagt aatttcgcct gcatccgtgg tgacatttcc cctatttcat ccaacagcac
    84841 cgaaccaccg ttcgcctgct caaagaatcc tttcttccct tccggagcat gaccaaacag
    84901 ttcactctcg accgcatctt ccggtataga cgcacagttc agcgccaggt aaggtttgcc
    84961 cgctctgggg cttgcctgat ggcaggcgta ggcaaagaga tctttacctg tacctgtgtc
    85021 acccgtaatc agcagcggcg cgcttagcat cgccagtttc tgcgcctgtt cgacaacatg
    85081 cttcattttc gggctgacgg cgacaatttg actgaaggcg ctgacgtcct gggcggcgac
    85141 attttgcaac tggcggccca tacgaatcgt tgatcgcaac atcaccaccg caccggtcag
    85201 gacgtgttga tcattttcat cctgaagata aacaggcgta atctccatca ggaaattctg
    85261 cccattaata acgacatgct cgttatggga atcttgcggt tcgctttcca gccaacgtaa
    85321 aaaattaaag ccgttaatca attgtgcggc ggtatggttg cgcaggcgat ccaatttttg
    85381 cgcaaaaagc tgacagctcg ccgggttcgc catatccact ttgcttttca tatcgacaga
    85441 gagcacaggt tcgggcaacg cctccagtaa cgcgctcaac gccagatgct cacgttcgga
    85501 aggcatccac gggacagtac gcacatcggt aacacctgca atacggcgta tttcggccat
    85561 cagactgctg aaactctcaa actccagttc agcaaaattg aggtagattc gcccaatggg
    85621 atcaatctca ataccgcgta aatcaatgcc tcttagcacg agtagatcga gtaattcgcg
    85681 ggtcagaccg agtcggtctt cacaaaagac ttccagacgc atgggaacct tcacctgaaa
    85741 aaagaacaat taatatgatg atatgacact atttggatag caggaaggtt tctgtcaaca
    85801 atcattgaca caaagacgga aaaacgggca cttcggcgta aagatatccg gtcagcaccc
    85861 gatattgcat tatttttcgc tgggcgtttt gcctttttgc agcgtttctt tcacctgacc
    85921 gataagctga cgacggaaat ccccgaggcg aggtttgtca tcgtcaatcc acggcagcgg
    85981 gcggcaaagc tccatagctt taatcccgag tcgtgccgtc agaagtccag caccaattcc
    86041 ctgagctgcg cgggtagaca aacgagcagc gagatcttgc gacatccagt ccatccccac
    86101 ttcgcgcacc agttcgctgg ctccggcaaa agcgatattc agcaatacca gcttaaacag
    86161 acgcaaacgg ctgtaatacc ccagttcaat gccatacagc gtggcgatgc gattaattaa
    86221 acgcagattg cgccaggcga taaacgccat atcgaccaac gccagcgggc tgaccgcaat
    86281 catcaacgtt gattccgccg ccgaacggct gatttcgcgc cgcgcctggg catctaaaac
    86341 tggctggacc aaatgcgcat acaaactgac cacttcacgg tcgttttgcg tttcatggat
    86401 tgaggcatac cagcgttgca gcgctggatg agactgatca atacccgcct gctgcgccag
    86461 tttttcgcaa aatgcgcggc ctttgcccgt gccgtggctg tgcaacaaat cgcgcgcttc
    86521 gtcgcgttca tgggcgcgct gtcgcaagcg ccataagcgc cgccactctg ttaccacaga
    86581 acctacgcca gcgccgataa tcaatgcccc agcggcacat ccacccagcg ccacccagtc
    86641 ctgagtttgc caggcattca ttgtccactg tacaccctgc ccgacaacgc ttgcgccaaa
    86701 cagagccagc ccgcccatca ccattttgcg ccacaggctg cgtttcggac gtaacgctgc
    86761 atccattacc gcttcaactt gcccctcttc ttcaggcgct tcatcgagcg tggccggggc
    86821 aaaattttgc gcctgatttt cgtcaaaggt ctgctgcgcc ctgaattttg gattctgatc
    86881 gacctccagc ggaccgtcga aatcaatacg tggttttaac ggttcggtca tcgcaattta
    86941 tctcctatta aaaattcaag cgcggcatca aggcgaatat gcggcagcgg tttgtcgaca
    87001 tccatcacct gcggacgaaa cgcctcaaac tggaagcctt gcttatccca gaacgcctga
    87061 ccgggcaaac gagcgggaac ttcgccagga taaacagtga gcggtgcgcc atcgctgagt
    87121 cgattaccgc gtagcgccgg gattttctca ccgttaacat caataatgcc gctggtggtc
    87181 gcctgcaccg aggccagccc caggcaatcc atactaatcc cttcaaacgc cgcattttgc
    87241 caggcatcct gaatcagttg ttgcagtaat gaaaccatat tggcgtgctg atcgatggtc
    87301 acatggtccg ctttagtggc agcaaacaat agcttatcga taaccggcga aaacaaacgc
    87361 cggaacaagg tacgctgccc gtagtggaaa ctttgcatca gctgcgtcag tgccagacgc
    87421 atatcattaa atgcctgtgg cccactgttg agaggttgca ggcaatcaac cagcacaatc
    87481 tggcggtcaa agcgcagaaa atgattctta tagaacccct tcaccacctt ctcgcagtaa
    87541 taattaaacc gctcgcgcag cattccggca ttggtgtgct tatcggcctg cgccagtttg
    87601 gactcgcccc aggcatcgac atccggccac gggaagaatt gcagcgcggg cgcacctgcc
    87661 atatctcccg gcaagacaaa gcgcccaggc tgaataaagt gcagcccctg ctgtttacag
    87721 tggtggagat aatcggtcca cgcggcagca atgtctgcca gccggttttc gtcggcaggc
    87781 gctagcgggt ccagcccttc gcacatcatt cgccatttga ccgaccattc gccgcgctga
    87841 ccattgagta agcccgtcat ctggcgtgac cagcttaaat agtcctgcgc cagcatcggc
    87901 aggtcgagca accattcgcc gggataatcc acaatctcca gatacagcgt ggaggtgtcc
    87961 ttgaagtggc gtagcagcga atcgttcgat ttaaaacgta gcgccaggcg gatttcactg
    88021 acgccgcgcg ttggcgtcgg ccaggcggga ggatcgccat ataactgcgc cagtccttca
    88081 tcatatgtga agcgcggaat gccaaagtca cgctgaggaa tgcgttttac gcccagcagg
    88141 cgctcttcac gcaccgcgct taacagcggc aagcgtgctc cggcgtgaat attgagcaac
    88201 tgattgacca tcgcagtgat aaacgctgtt ttgccgctgc ggcttagtcc ggttacggcg
    88261 aggcgcagat gtctgtcgac accccgattc accagcgcat taagttcatt tttaagtcgc
    88321 ttcattgctg tcctgtgctg ctctgaagac cagaaaaaaa tcattcgtaa ggatatgaat
    88381 gtgggggcca ggccattgat tatcaactgc ccgtgttgtc atcgacgggc gaactccata
    88441 agatgtgcag cattcacgtt cggacggagt tcctgaccta tgtcccctac tatttatgat
    88501 attgccaggg ttgcaggcgt atcaaaatcc accgtatcac gcgtgctgaa taagcaaact
    88561 aatatctccc cggaagcgcg cgaaaaagtg ttacgggcca ttgaagaatt acagtatcag
    88621 ccaaacaagc tggcccgcgc gctgacctct tcgggttttg atgccattat ggtgatttct
    88681 acccgttcga ccaaaactac ggcgggtaat ccgtttttct ctgaagtttt gcatgccatc
    88741 accgccaaag ctgaagagga aggtttcgac gtgatattgc agacgtcgca caatccggca
    88801 gaagacttac aaaaatgtga aagcaaaatt aagcagaaaa tgattaaagg cattattatg
    88861 ctcagttcgc cagcggatga gtcatttttt gcccaactcg ataaatacga tattccggtg
    88921 gtggtaattg gcaaagttga aggtcaatat gcccatgttt attctgtcga taccgataat
    88981 tttggcgaca gcattgcgtt gaccgatgca ttaattgaaa gtgggcatca aaatattgcc
    89041 tgcctgcatg ctccgcttga tgtccatgtt tcggttgatc gcgttaatgg ctataagcaa
    89101 agtctgtccg cgcataatat tgcagtgcgt gataaatgga ttgttgacgg cggttatacc
    89161 catgaaacag cactgcaagc agcacggcaa ttattaagtc agtcaccgtt gcctgaggca
    89221 gtgtttgcca ctgacagcct gaaattaatg agcatttatc gtgcggcggc agagaaaaat
    89281 attgctattc cgcagcagtt agcggtggtg ggttatagta atgaaacgct gtcgtttatt
    89341 ttaacgcctg caccaggcgg catcgatgtt ccgacgcagg agttaggtca acaaagctgc
    89401 gagttattat tccgcttaat ttctggaaaa ccgtcaccac agaatattac cgttgccacg
    89461 catatgacgc tgaaataaat accagctacc ttgcctgaat gccggatgcg atgctagcgc
    89521 atcttatccg gcctacattt atagatcgga taaagtacta gcgccgcatc cggcaattta
    89581 gcccattatc agaacgagta atttacgccg acacctgcat aatggaattt atcgctgtcg
    89641 ccttcgtcgt gatcctgcca ctcaaacgcg tattccagcg aaacggaaag accattctgg
    89701 aaatcataac cgtaaaataa acccacacgg ttaaaatcat ggccttcacg ttcgatatca
    89761 tcctgccagt cccagttact ccagcgatcc agcccaatgc gcgtatacgg cgtcaccgag
    89821 tggttgccga gcgtcagcgg caaataggcg cgaatttctt gcgtagaaaa ctcaccgtta
    89881 ttgcggctgt cgtccatatt aaagccgcgc tcgagataat agttcactcg caaggcaacc
    89941 gtttcgttga aggtatattg caggcctgtt tccgtttcga ctcgggtatc agcgtaaccg
    90001 gtcgtgttca gatcgttggc aaatttatac atcgacaacc aaccgttgaa acgtaaatcg
    90061 tcagtcagtt tcacatccca gtctggcgcg attttccagc gctgcatatt cgccgtgtct
    90121 ttacccggtt catcaacgta gtgataacca taattacgga aaccgccggt caggccgaaa
    90181 ctgaaatcat cgttttcgag gaactgataa tgcacctcca gctccgggcg atcaaaccac
    90241 gttccacgtt tacccgcgct ataatctacc ggcccttcct gataataggc cagagcaatt
    90301 ctccacggcc cgttggcggc attaaaatag actgaaggct ccgccagccc atccatatct
    90361 tcgccataac cctcgacgtt ttctatttcg tacatcgcgc cgatattaaa gtgccagtcg
    90421 ttcctttcct cggcctgtgc gcaggccatt cccgcacaca tcaccagtgc ggtacagggt
    90481 aataactttt tcattattgt tccttgtatt attctggggg aatgccttcc ccctgtattt
    90541 agcgaattgc tatttccgtt tctgcatcaa agaaatgaca tttcgtcata tcaaaatgaa
    90601 tagtgatatt ttctcctgca tgataatcat ttaacgcacc agcacggacc actaactcgt
    90661 gccccccaac cgtggtgtag agcataaatt ccgcaccggt taattctgcc acgctaattt
    90721 tggcggaaat gttattttcc tcttgcgcgt ccggatgaat atcttccgga cgtattccca
    90781 tcacgatggg cttatgcaaa cttccctgtg ttttcagaac cgctaatttc tcttcgggaa
    90841 tggttaattt aagcgtttcc gtaacgaatt tatcgccatc gatcgtgcca cgaataaaat
    90901 tcatcgccgg tgatccaata aatccggcaa caaacatatt cgctggttgg ttataaaccg
    90961 ttttcggcgc accaacttgc tgaacaatcc catccttcat aatcacaatc cgcgtcgcca
    91021 tggtcatcgc ttcggtctga tcgtgggtca cgtagatcat ggtggtattc agcttctggt
    91081 gtagcttgct gatctctgcg cgcatttgca cgcgcagctt ggcatcaagg ttagagagcg
    91141 gttcatccat taaaaacacg cccgcttcgc gcacaatcgc ccgcccgagc gccacacgct
    91201 ggcgttgccc gccggaaagc gcgcccggct tgcgtttcag gtactcacgc aggccgagaa
    91261 tttgcgccgc ccagttcacc cgctcatcaa tcacctcttt ggcgattttt tgcatcttca
    91321 ggccaaacgc catgttgtcg taaaccgtca tatgcggata caacgcgtag ttctggaaca
    91381 ccatcgctat attgcgtgct ttggctggaa cgtcattcat tcgtttgccg tcgatcaaca
    91441 gatcgccgcc gctgatctcc tcaagcccgg caatcatgcg cagggtggtc gacttaccgc
    91501 agcccgacgg gccgacaaac acgatgaact ctttatcggc aatttccagg ttgaagtcct
    91561 tcaccacatg cacctggtta tcgtagattt cttgaatatg ttgtaacgaa agctgagcca
    91621 tgttgattcc tttgctatac gttttgccag aaggccgata accgcgacca ggtcagagag
    91681 tccgttgaag gcaacagtaa ttgcgccccg gttaagcccg cgccgatccc caccgagcgc
    91741 ataccgctgg cgttaatggc gtcaatgccc gcctgcgcat cttcaatgcc gatacatgcc
    91801 tgcggcggca cgcccagccc ggcacaggca gcgagaaaga tttccggatc cggtttcgag
    91861 tttttaagct gggaagcatc cgcgcagaag gtgaaaaact cgcgcagctc cagcgccgct
    91921 aaaatcgtcg gcgcattcag ggagacggaa gccagcccta ccggaatctg ctgtgcacgg
    91981 agctctgcca gcaaattgcg aataccgggc agcacagcat taaccgtcag ctcgcgcagt
    92041 gagtggacgt aaagcagatt tttgcgatac gccagttgcg cccgctcctg cgggttaaag
    92101 tcgccctctt tgcccccgtg ttgcagaatg cgccgcagag actcatcgcg gctgatccct
    92161 tttagggatt cgttaaactg cgcatcaatg ctgatgccaa tttcagcggc aatctgctgc
    92221 cacgccagga aatgcagatg cgcggtatcg gtgattacac catccagatc gaaaattacc
    92281 ccttgcagtt tcattcaccc tcctgatgcg tggtagcggt cccattgaag ggcaaaataa
    92341 aatcacccaa acagaaaaca gattcttcag ccacggatat aagctgaccg ttcaaacgca
    92401 gtgaaacggg cgcagaagtt cgaatcgcaa tacgctgcgc gtcgagagtg acctgtaatt
    92461 cgcagccctg ccagaacaga gggaaagaca actgttgcca ctgctcaggt aacgccggat
    92521 tgagatgcaa ttcaccgtca cgcacgctca ccccggcaaa accctgaatc gcccccagcc
    92581 agatagcgcc agttgcggca gcatggatac catcatcaca actatgcgga tcagcaccaa
    92641 gatcgatttc agtcccctcg caccagaact gataactttg ggtcagcagg ccacagcgtg
    92701 cggcaacaat gccgtggatt gctttactta atgacgagtc gtgaatagtg cgcggttcat
    92761 aaaattgcag attggcaaga cacgatgccg ctgagaactg ctccggcagc atgtaattga
    92821 gcatcaccac atctgcctgc ttgaggatct gcatctcgtt cacttctgcg cgtgaataat
    92881 ccagcagtat ggtttgcttc cccgccgccg ctttgtattt cgccaggtta atcgccggct
    92941 tagccataaa cgaatcatcc tgcggcaaaa cgccgtcggg ctgaatttct ggcagccgca
    93001 gttctttaag gaacatttcg gcacgatgaa taaacgcgtc gtcgctacag ccaaactggc
    93061 gggcaatact cagcgcctgc tggacgttgt aatacgccat atagctggtg aaggcgttat
    93121 tattgacatg ttcggtatat tcgtcaggcc caataacatc atgaatttcc agacggtcgt
    93181 taaccctcac cgcgcggcta atccagaact ttgcagtttc cagaagtagc gccatgcctt
    93241 catgcgcaat gaaactttca tcccccgtgg tctgccagta ttgaataacc gcccaggcga
    93301 tatcggccac cagatgatgt tccgcctgcg ccgaggccac tttttgccgc agcccggtgc
    93361 gaatgttaat ggcggcaaaa agtggcgtct cttcttcgcc gctgcgcgcg ctttcccacg
    93421 ggaatagcgc gccctgccag ccattgcgtc gcgctttctc ctgcgcgcca ggcaagttgt
    93481 gccagcgata acgcagtaaa cttctggcaa tcgtcggatc gctaaacaga tggaacggca
    93541 gcaaaaatac ttccgtatcc cagaaaacgt ggcctttgta gccttcgccg gttaagcctt
    93601 ttgccgcaat gctgctacgc tcgtcgtgag caggcgtcat gatgcgcaga tgataaagcg
    93661 cataatctaa cgcttgctga tcgtgcgcat cgccgccgtt taccgtgata cgacgtttct
    93721 gccaccactg acgccagttt tctgttgatg ctgcaagaag ttggtcgtaa ctctgctgcg
    93781 cgcacatttc aagctggcga agcgacgcgc tgccccactc gtctaaaacg gcttgcctgt
    93841 catcccgcca gtcgatccac accagttttt gcaacgtcac tgtctcgcct gcatgaagct
    93901 gcgcactggt atgttgcagt aaacggcgct ctttggcggt atagcatttc tgcacatcac
    93961 cgctcacctt acagcaacag ctgatggcca catcactgcg tccatcctgg gtggtgtaga
    94021 tcccctgcat caaatgctga ccaaacaccc gcacctgggt ttcgtcgaga tgttggcgac
    94081 cgtggttggt ttgcgtggcg tcgatacctg ttgaaatcag cactgacgcg tcggcgtcca
    94141 gtggcgtaat agtgatttcc agcgcaatga gcggcagttg gtctgccgaa acaaagcgac
    94201 ggctggcgat ggtgtaacct gcgccgttgc tggtacgcca gacaacgttg cggcgtaatt
    94261 cgccactggc aaagtcaagc tcacgctgcc aggcttcgtg ggataacgag aaaacctcac
    94321 catttatggc aatctccatc cccaagatat caggcaggtt caccagttcg ttgatttcac
    94381 ctttacccgc ccgatgatac agccccgcca gatacatccc tcgcgtctgg cgcgtgtaat
    94441 cttcttcatg gctggcgcga agcccaagat agccgttccc ctgcgccatc agcgatgcat
    94501 acttgttcag ggtatgctgg ctaaaatggg gttctgataa cgttactggc ctggtcataa
    94561 ttccacacgc gtccctgttt ctgcggattg atataacgcc gccaccagtt gctggatgat
    94621 gtacccctgc tcggcgtcgg ctatcatcac gggcttgccc tgtacgtgat tgataaaggc
    94681 ttccatgctg cgcaaatggc ggttgtcgtc tgccatttcc cgttgcatca gcgtcattaa
    94741 ttcaccgttg ttatcggtgt agatatgtgc tggaaacagc gtcgcaccgg ctttatcacc
    94801 acaaaagctg acgttcataa tcgactgttc gcggatgttg agtgcaaatg acgtttccag
    94861 ccacaggatg ccgccgttat gaaattcaat ggtgccaaac agcgaatctt cgacgctgta
    94921 agttgccgga tcccactcgc caaattgacc acagctcttt tgcgtgccga tcttttgaaa
    94981 gctatgcgca ttcacgcttt tcaccgccgg aaagcccagc acatacatcg cagcatccag
    95041 catatgaatg ccgatgtcga tcagcgggcc accaccctgc agttctttat tggtaaagac
    95101 accccagccg ggaacgccgc agcgacgcag ggcgcgggcg gtggtaacgt aaatctctcc
    95161 caaaacgccg ttggtcacct gttcacgcag ctgttgcgta tcgagcgtaa aacgatggtg
    95221 aaagtcgtag gccagcacct tgccctgttt gcgcgcggta tcgcacattt cccgcgcctg
    95281 ttctggcgtc atggcgggcg gtttttcgca catcacatgg cagcccgctt ccagtgccat
    95341 cagggtatgt tcgtaatgaa aacggttagg tgagcagacg ctaaccacat caggtttcac
    95401 cgccagcagc atggcctgcg gatcgtccca cacggatgca ttaccgtatt tatctgccag
    95461 cgcctgcgcc tgggaaaggc ggctatcaca gacagccacc agttccagat cgttgcgggt
    95521 gcagtagtac gaagcatgaa ccttatccgc cacctggcct gcgcctatta tcgcgacccg
    95581 cagcggagag cttgtcattg cacttttcac aaagacctct tagcaggtac gcaaccaggc
    95641 caacgaatca cggtacgcct gggcaggatc ttctgcccgg atacgccctt catacaccac
    95701 ataaccctga tagttatcgg cgcgcagctg ttcaaacagc gcgtggaaat cgagggtgcc
    95761 actgcccggc tggtaacgat ggttatcggc aatatgcaca tgaccgagca agtcacggtt
    95821 gtcgtgcagc gcctgcgcca ggttatcttc ttcgatgttc atgtgataga aatcgccgat
    95881 aatctgcaca tgtttaagat tgttttcgac gatgtaacga cgagcatcgg cgagggtgtt
    95941 gatcatatga tcctgatagc ggtttaacgg ttcgagatac accacggttc cggtacgcgc
    96001 ggcgacctgt tccagtacgc gcaccatttt gcggtcgccg tccaggctac gcggcgaggt
    96061 catcggcggt aagcggaagg taaacatgcc ccacgctgcc ggaacgacga tgcctttacc
    96121 ccccacttct gccagcgctt cgagaatgcg ttcgatctgc tttaagccat taagacgacg
    96181 ctcttcgata aagtcaccaa tccagccgtc atagccaccg caggcggtgg tcaccggtaa
    96241 accagtttct ttgatcgccg ctttgacttc ttcgagattg ttgaccagca gtttgccgtc
    96301 gatctcaaaa ccatcgaaac ccatctcttt gatataacga aatttctcca gaatattttc
    96361 cggaaagaac gcctgattct gtgtgcctat tttcattttt ttcattcctt tgaattaaaa
    96421 cgtaacgccc attttgatgc tctgttccgg atgctggtcg acatactgca tgtagctttc
    96481 cgggctattg gcaaaggtca ccaccgggtc gattagatct tcgcaattga gataaccgtt
    96541 catcagcagt tcccagcagg tttcttcaat acgcttacgg ctccagcgcg gataatccgg
    96601 gttcggttcg ctgcacgcgc gggagaaaac aattttggcg ttattgaaat gcgcttcgcg
    96661 tccgaggtta aaaccttcag caaatggttt ggcaaacgcg acataagaga tggtgccgcc
    96721 ataagcaagg ccacgcagcg ccgattgcag cgcgtcggcg taaccgctgg tttcgataat
    96781 cacatcggca ccctgcttgc cggtcagcgt tttgatctct ttacctacgt cagtgccgat
    96841 ggggttaagg cagaaatccg caccgtggcg acgggcaata tcacagcgat gggcgatagg
    96901 atcgacgcca attaccaccg atgcgccagc gcgtttcgcc agttggatgg ctatttgacc
    96961 aatcgcgcca agccctacca ccaccacaaa atcacccacg cgcacgttgg catcgcgcac
    97021 gccgctcatg gcaaactgcg ccgggtcgta acagacggcg tttttccagg agctgccttg
    97081 cggcattttg cgcagcttgt agttattcac cgcgttaatg atgaccgtct cggagagtgg
    97141 gccgtagccg catacgctgt cgcccaccgc gtagtcggtt acgtcgctgc cgcactcgat
    97201 aatgtcgcca accaccatgt tgccaagctg gaatttgcca aactcaatgc cgcgcggcgc
    97261 atctgccgga cgcggagtga acatctgcca ttcaccgtta aagtcttcat cgataaacgg
    97321 gctggcggcg cggaagtcga ccacttccgt tccgtgtttc ggtgcgccga aacgcacgcg
    97381 gatcttaact tcattggcta aaatcgcccg atcttcatac tcaaccagcg cagcaacgcg
    97441 cggtgctgtg gctactaact ttttcattta aaactccttg taaagaaact ggccgcttta
    97501 gcccttcacg ccaccggcgg tcaaaccact cttaataaaa cgttccgaaa gcgcatacat
    97561 aatcaccacc ggcaatgcgg tcaccagtga ggccgccatc atccgtcccc agatgtaatc
    97621 tggcgtactg aacagtgcgt tcaggcccac cggtaaggtg aaattgctgg cgctggaaag
    97681 gaaaatcgac gcaaacagat agtcgttcca cgccaccata aagcagtaga cgaacaccga
    97741 tatcagcccg gacatcgcca ggggcacggt gatacggaag atgatttgca gacggttgag
    97801 gccatccatc atcgccgctt cttcgatctc atcagggatg gtgtcgaagt agcttttcag
    97861 cataaacacg gcggtgggca gggtttgcgt caccatggta atgatcagcg ccatctcggt
    97921 gtcgtaaatg cccagcgcgg tgatgatttt gaacagcggc accaccagca aaatgccaga
    97981 gaacatatac accgtgtaaa agctggcatt gatggtcatc cgacctttaa agcgcaggcg
    98041 ggaaagcgcg taagccccaa gaatgccgag gaataccgcc accacggatg aaaccaccga
    98101 caccaccaga ctgttacgga agtagtcgac aaacgggaaa atcatcgggt taaaaatgtc
    98161 gacgtaatgc tccagcgtcc actgctgcgg cagcagcgta ggatgcagtg agatcgcctc
    98221 tttcgcgccc ttgaacgagg tcatcagcat cacaaaaaat ggaaacagcg tgatgatgag
    98281 aaacagcgcc agcccgcagt aaaaaccgat gcgactgagt gtgcgtttat ttgttgccat
    98341 tgaggttcac ccgtttcctg gtcagcagaa tgacagcgaa aataatgatg aagagcacca
    98401 ccgagatcgc cgccgctttt cctaaatcat taaaggcaaa ggcggttttg tagagatata
    98461 caccgagaat atcgaccttg gtcgtcagca aataaacatc cgcgaacatg tagaacatcc
    98521 agatggtgcg cagggtcacc accgtcgcca ggaccggcat aatcgcgggc agcgtgacga
    98581 tacgaaaccg ttgccaggca ttagcgccat ccatctctgc cgcttcgtac agcgatttgt
    98641 cgatggtctg caaaatcgcc agaaacgaga taaaggcata cgggaagtag cgccagatgg
    98701 cgaacagcac caccagcaca aagctactgc ccggattatc gaaccacagc ggtgcctgct
    98761 catagagatg cagaagatcg acgccgaggt agttcacaat gccgtagccg ttgttgaaca
    98821 tgtatttcca ggcaaacacc agcgaaatgg acggcgttac gtaggagagg ctcaccagcg
    98881 aacgcgcggt tttgcgcagg cggaactcgc ggttgaaaaa catcgccact gccagcccga
    98941 gcacggtgct gcccgccacc accagcgcgg tataccagac cgtcatccac agcgagtgcc
    99001 agaagccggg atcggagagg atacgcacat agttactcac cccaacaaag gttgactcga
    99061 tgttgggatt gagcggcaga cgtaaaaaac tgatttcgat attcgacacc attggccagg
    99121 ccaccagacc acccagcagt aataagctgg gcgcgagaag cagcagcgca aagggcatat
    99181 cggaacgacc tgaaaaaagc ctgttcattg cggattcctg tcctgggttt agtgctgttc
    99241 gatcagttca tccagctttt tctggctcgc ttgcagcgtg ccagggagat cggctttacc
    99301 cacggtgacg ttatgcacca tggagctcac cacgccagaa cccgtcacat cacccatgcg
    99361 ggtaaagttt ttatccccta ctgcaccaaa gacctgaata tttggcagtt caccgattag
    99421 ctgattcggt agttcaccca gcgccttaat aacgtcgttg tctttccagg tggcggtagt
    99481 gacaaccgct ttattcaccg gcagcgcagc acctggcgac atcatcaccc agtcggcaat
    99541 gttgtctgcc tgctccataa aggtgacaaa tttttcagct gcttccgtct cttcggtctt
    99601 ttgcccggcg gtaatggtca gcgaggtcaa catgccgtag accgcagagt ttttctcggt
    99661 tggcaccacg aaaccgacgt ttttcgggtc gccttcttta atcacagccg gaaggatata
    99721 ggtggagtaa atcgccatcg gcgcggtgcc gttcataaag gcgtctttca cttccatgat
    99781 gtcgttagaa cccggcatag tgttggcagc aaggtcgcgg taataggtca gtgcctgcat
    99841 catctctggt gtatcaaggg tgattttgcc ttcggcgtta aagacgttag cttggttgga
    99901 taacgcaaac tgggagaagg attgttccgt caacacgctt tctgctgtag gcagcgcaat
    99961 gccgtatttt ttattcgccg ggtcattcag tttctgtgca acgtccagca gctgttgcca
   100021 gtttttcggc tcctcaagcc ccgcttttgc cagcacatct ttgcgatacc agataccgcc
   100081 aatccaggcg ctgacaggaa caccggtcca tgcgctacca tcttcggtac gcacaatacg
   100141 cagtacgcca tcgtaaaacg cgccttcacc aacgttgctg atgactgtgg caaccgcttt
   100201 gcgatcgata agctgctctt tgtccatcac tttggcgtag tcatggctgg tttctatcac
   100261 ttccggcagc gaaccgctac gtgaaagagt aatgacttta gtgttatagg catcttcttc
   100321 cacgggcact tgcttaacgg tgatgccagg gttttctttt tcaaaacggg caatcaattt
   100381 actgataacg gcctggcgct cctgctccac cgaagaatgc ataaattcga tggatacatt
   100441 cgttttattt tcttctttac agcctgaaat cagggcgcag gaaaccagtg ctgataacag
   100501 cacgattttt gatttaatca tgagatgttc ctttttattt aatccacata acctgccagg
   100561 ggcgcagcgt tatttcatta cccaatatat caacttcact aatcagatcc cgaccatgca
   100621 gattagtaat atttacatgc tgaatatttt tactgacatt aaacaatcca gtcagacaat
   100681 taccatcagc gttacttctt tgaatacgca ttacggatga attaatcgta tcgatggtaa
   100741 aattattatc cggatgaaac tcgttatggc tgcgacgaag tgtaattaaa cgcgacaact
   100801 catgatatac cgcatgcctt aatgtagctt catcgttcag ttctcgggtt atctctttac
   100861 tgtgatattt tttacggtta atcgcacggt tatatccgag tttttcgaca cctgcgtaat
   100921 cattacgcga gccaagaata ctttgaatat atatcgctgg cacaccgggg aaacttaaca
   100981 aaatcgcatg ggcgagaata aacctggcgc aacgttcttc atcgctactc tcacggcggc
   101041 ttaacgcatc catataggtc acatttattt catacggact gcgtgtaccg tcgggattat
   101101 ttttccagtt tactaatgca ccttcctgct gtaacgcctc gaccagctct aatatttcgc
   101161 tttcaggcaa caagccccgt agcgggttta gcccgatgcc atcgtgagag gcgaggaagt
   101221 taaaccaggt ggtgttgctg gaaggtagtg tcaggttttg cgcccacgca caaagcgcct
   101281 caacgttctg tttttgcacc gcatgcagca ccagtggcgg cagcgagaac tggtacacca
   101341 tatgtgcttc gtcatcgcct gcgccaaagt aagcaatgtt gtctttatgc ggaacattgg
   101401 tctcggtaat gatcactgta cctggcgcaa cgttatcaat aatcgaccgt aacagtttga
   101461 taatcagatg tgttttttcc agatggatgc agcttgttcc cggctctttc cacataaagc
   101521 caacggcatc caggcggaca tactcagcac ctttcgcaag gtaacagagt aaaacatcca
   101581 ccatcgccag caacacttca gggctacggt aattcaggtc aatttgatcg tcactaaagg
   101641 tggtccataa atggcgcgtt gaatgatcgc gcatctggaa tggcgttaat aacggtaacg
   101701 cacgcgggcg agtgacggcg ctgagatcgg tttgcgggtc aacggcaata aaaaaatctt
   101761 caaaacctgg atgctgttgt aaatagtttt taaaccattc actttttgcc gacatatggt
   101821 tgcagacaaa atcaaacatt aaatgactgc attcaccgag ttgctgaata tcctgccact
   101881 cccccgcttc actggcgacc tgatgataat caattaccga aaagccatca tcagatgacc
   101941 atggataaaa cggcaacaaa tgaacatgtg aaaaaatgct ttgcagccat tgatggtaaa
   102001 actgattaaa tgtgggtaat ggttttaaat cattgctgtg aaattgatcg gcataggtaa
   102061 ttaacacgac atcactttca ttccagtgtt ttttacgtcg ctgtgtaatt aatcgtttcg
   102121 cactctcaag acgcgttaca agtttctgta aatgagttgc ggtaaatgtt ccaccgtaga
   102181 tttcgtccag gtaatccgta attttctgtt tcatgttgcc tttccgtggt agcggtccca
   102241 tgaaacgaga gactactccg cagaaaataa actgtcaatt gagcacaagg gttttagtca
   102301 cgcaggaaat cattttttac cagggaaaaa gcgtatttgt gagctgacgc aaataatcta
   102361 tggggatgta aataaggtgt gacagaaaaa aaacggcgca taagcgccgc tcatggtgaa
   102421 ttcttttaac ctttgacctt cggcattgcg atgtctttca ggccaccggc gttctcaacg
   102481 tgggtatatc ccatctcgct aaggatctct tttgcttgcc ctgactggcg tccggcattg
   102541 caatacactt tcacggtgtc gtttttatcc ggaacggcgg tggcaatgcg ctctttcact
   102601 tccttcaggg gaatattgat ggccccctga acgtgctctt gctgatactg ctctggaaca
   102661 cgaacatcga tccagtgttc agcggcgaaa acgggcagtg aaaacaccag tgccagagca
   102721 agtaagcctt ttttaaacat gtaagcgtcc ttctatggat gaaaattgcc atccattata
   102781 cgcctgagat ttaacaattt ttgcgaaact cccctcacct tttataacgc tgtgccagtt
   102841 tattagcagc ccgacttaac agcggttcca gcgccacagc cagcaacatt ttcagcggtc
   102901 ggcgagcaac tgattttatc gcccagcccg ctacacccgc cgggccatag cgcagtgcgg
   102961 taagaagtac cagcttgcct gctaatttga aaccaggctt taccttttgc ccggcctgtt
   103021 gccagcgagt attcataact ttcctcacag ttgacggaaa cggctacgta acgtgaaagt
   103081 atcggaagtg acataacgtt ccatctcgcg taaacgcgtt tcacttgccg ccagttcacg
   103141 gtcgacttca tccagcaatt cgctgctgga aggtagctgc tcaccaaagg ccatgttgtc
   103201 cggcattgga tcaagcgcaa atgacaaaat gatgtaagca accagggtaa acagcgccag
   103261 accgaagaaa atcgacagca ccaccaggat acgcaccagt tttgccggta catcaaaata
   103321 gttggcaatc ccggcgcaga cgccgcggac catgccctgc tgtggaatac gccataattt
   103381 tttattgaga ttaatgcccg ccattagcga tccctccagt tcggatgttc tgcatcaaga
   103441 atagattcca gcgcctgaat acgttcgcgc atccgttttg cttcatcagc cagttgcgct
   103501 aatcgctgct gctcactttg cgacaattca ctgcgaccag aacgattgct gtaatgcagc
   103561 cataaccaga tcggtaaaac aaacagcaca aaaatggtta acggaatagc cagaaatagc
   103621 gcgctcataa gtactcctta gacgatgagc ggcggcgcgt tcggacgccg cctggatgtt
   103681 attattgatt gtcttgcttc attttggctt ttaattgtgc cagttgttcg ctgattgcat
   103741 catcggcttt cagttcggca aactgatcgt ccagcgattt ttgtttaccg aagctgtggc
   103801 tttctgcttc cgcttccatc tggtcaatac gacgttcgaa agattcgaaa cgagccattg
   103861 cttcatccag tttgccactg tccagctgac gacgcacatc gcgcgacgag tttgccgcct
   103921 gatgacgtaa catcaatgcc tgctggcgag cgcgtgtttc gctcaatttg ttttccagct
   103981 caccgatctc tttcttcatg cgtgccagcg tgtcgtccac cagcgtcact tcatgttcca
   104041 ggctcttaat cagatcggtc agtttctgtt tttcaattaa cgctgcacgt gccagatcct
   104101 ctctctcttt cagcagcgcc agttcggctt tttcctgcca ttcaacttca cgcgccgacg
   104161 cttgttcaat acggcgagtc agctgtttct tttctgccaa cgcgcgcgcc gaagtagaac
   104221 gtacttcaac cagtgtatct tccatctcct ggatcatcag acgaaccagt ttctgtggat
   104281 cttccgcttt ctctaacaga gcgttgatgt tggcattcac gatgtcggca aagcgagaaa
   104341 aaatacccat aatgttgtcc tcttgatttc tgcgttcagg attgtcctgc tgaactgtta
   104401 atacaatttg cgtgccaatt ttttatcttt ttgatttata aagatctgat tgaagaatca
   104461 acagcaacat gccaggatga gttagcgaat tacactaaca agtggcgaat ttcatcatgg
   104521 cagaatacaa agataattta cttggtgagg cgaacagctt tctcgaagtg ctggaacagg
   104581 tttcgcatct cgcaccgctg gacaaaccgg tgctcatcat cggcgaacgc ggcaccggta
   104641 aagagctgat tgccagccgc ctgcattatc tctcctcccg ttggcaaggg ccgtttattt
   104701 cccttaactg cgcggcgtta aatgaaaatc tgctggattc cgaactgttt ggtcacgaag
   104761 cgggggcgtt taccggtgcg caaaaacgtc atccagggag atttgaacgt gccgacggcg
   104821 gtacgctatt tcttgatgaa ctcgctacgg caccgatgat ggtgcaggag aaattattgc
   104881 gcgtgattga gtacggtgaa ctggagcgcg ttggcggcag ccagccattg caggtgaatg
   104941 tgcggttggt atgcgcgacg aatgccgatc tcccggcgat ggtcaatgaa ggcacttttc
   105001 gcgctgacct gctcgaccga ctggcttttg atgttgtaca actgccacca ctgcgcgagc
   105061 gcgaaagcga cataatgttg atggcagaac actttgccat ccagatgtgt cgggaaatca
   105121 agctgcctct gttcccgggg tttacggagc gcgccagaga aacattgctg aattatcgtt
   105181 ggccgggaaa tattcgtgaa ttgaaaaacg tggtggaacg ttcagtgtat cgccacggca
   105241 ccagcgatta tccgcttgat gacatcatta ttgatccctt taaacggcgt ccgcctgaag
   105301 acgctatcgc cgtttcagaa accacctcgc ttccaacact gccgctggat ttacgtgagt
   105361 ttcagatgca gcaggaaaaa gagttgctgc aactcagttt gcaacaaggg aaatataacc
   105421 agaagcgcgc ggctgaatta ctggggttaa cttatcatca gttccgcgcg ttgttgaaaa
   105481 agcaccagat ttagcgaagt tgagcaatac aacttgccgg atgcggcgtg aatgctttat
   105541 tcggcataca taatcaatac gtcgtttgca ggcctgataa gcgcagcgca tcagacatta
   105601 ttgcgttggg ctattaatcg ctcagcgcat cctgcaaaat tttcattgcc gcatcgaatt
   105661 gcacatccgg gatggtcagc ggatacagga agcgaatcac gttgccgtat gcgccacagg
   105721 tcagcaggag cagcccctgc gccagcgcgc gttgctggat tttctgtgca atcgccgctg
   105781 acggcttgcc cgtttgcgga tcgttaaact ctgccgcaat catcgacccc aggccgcgta
   105841 ccgcagcaat ggccggaacg ctttctttgg catcaatcaa cgtgtttttg agacgctggc
   105901 ccagttgatt cgcgcgttcg cagagtgatt ctttgtcgat aatattgagc accgcgtgcg
   105961 cggcagccac cgccagcggg ttcccggcgt aggtgccacc caacccgccc ggcgcgggcg
   106021 cgtccataat attcgcgtta ccgaccacgc ccgaaagcgg catcccgccc gcgaggcttt
   106081 tcgccatcgt cattaagtcc ggcttatcgg cgtaatgatc catggcaaac agtttaccgg
   106141 tacgcgcaaa gccgctttgc acttcatcgg caatcatcac aataccgtgc tcgtcgcaca
   106201 ggcggcgaat ggcggcaacc agctcttttg gcgcaacgtt gaaaccgccc tcgccctgca
   106261 ccggttcgaa aataatggcc gccacctgct tcgcttcaat gtccgattta aacaagcgtt
   106321 cgatggcgtc gagggagtcc tgtgttgaaa tgccgtgtaa atctgacgga taaggtacgt
   106381 gatacaccga accagggaac gggccgaagc cgattttgta cggcgcaact tttccggtca
   106441 gcgccatggt catatatgta cgaccgtgga agccgccgct aaacgcaatc acgccaggtc
   106501 gtccggtatg ggcgcgagca attttcaccg cgttttccac cgcttccgcg ccggtagtga
   106561 agaatgcggt tttagcctgc ccgctcactg gggcaagggc gttaattttc tccgccagcg
   106621 tgacgtagct ttcgtacggc acaatctgat acgccgtgtg ggtaaactgt tgcaattgct
   106681 gctccaccgc cgcgaccaga tcagggtggc gatgcccggt attcagcacc gcaatgcctg
   106741 cggcgaaatc gatgtattcg ttgccctcta cgtccttcag cgtagcgttt tctgccgact
   106801 gggcgaagaa gttacacatc acgccaaccc cgcgtggggt agcagaaaga cgacgctgat
   106861 ggaattcatt gttgctcatg tttcacacct tttgactctt cactgttaat cggaaattaa
   106921 acgcccagac gatcgcgcag gctgtaataa gccgcgccca tcgcggtaaa tggcacgcgc
   106981 agcgtgcgcc cgccggggaa cgggtaatgc ggcagattgg cgaaggcatc gaaacgttcg
   107041 gcgtcgccgc gcagcagttc ggcaatcaga cgcccggcaa gatgggtaca ggtgacgccg
   107101 tggccgctgt agccctgcat gtaatagatg ttggtatcaa ggcgaccgaa ctgcggcatg
   107161 cgcgacaagg tcagcaggaa gttgcccgtc cagcggtaat caattttcac gcccttcagc
   107221 tgcgggaagg ttttcagcag tttcggcacc acaaggcgct caacgtcatc cgggtcgcgc
   107281 gcgccgtaga ccacgccgcc gccgtacagc aggcggttgt cggcggtaag acggtagtaa
   107341 tcaagcagat agttacagtc ttccacacag tagtttttcg ggatcagcga acgggctaaa
   107401 tcttccgaca gccgttcggt ggtgatcacc tgcgtgccgc acggcatgct gcgtttcgcc
   107461 agttccggct ctactttatc gcccagatac gcattcccgg cgacaatcac atacttcgcc
   107521 gtcacctggc ctttggcagt gcgcacaacg gctggcgtgt tgtgctggat ctgcgtcacg
   107581 gcagaaagtt catacacgcg cccgccgttg aggcggatgg cgtccgcttc gccaatggcg
   107641 aggtttagcg ggtgaatatg cccaccgctg tgatccagca gcgcaccggt atagcgatcg
   107701 ctggctactt cacggcgaat ggcgttggcg tccagcaatt ccagctgttt attgccgtag
   107761 cgttcccagt tctctttctg ctcttcaagt gtggcgagct gtttatcatt catcgccaca
   107821 aacaggccgc cggggcggta gtcgcagtca atctgataac gtttgatacg ttcgcggatg
   107881 atctcaccac cttcgaacat catgctgccg agcatacggg cggtgtccat gccgtagctt
   107941 ttttcgatca cgtcgatgtc gcggctgtag gagttcacaa gctgcccgcc attgcgcccg
   108001 cttgcgccaa agccgatgcg tgaggcttcg agaaccacta cgtcaaagcc cgcttccgcc
   108061 agatgcagcg cggaggagag cccggtatag ccgccgccaa ccacgcaaac gtcgcaggtg
   108121 attgactcat tcagcgtgtc gaatggcgcg tatttattcg cactggcggc gtaataactg
   108181 ctggtatgtt cggtcatttc aggcctccag gcttatccag atggttttca gttcggtgaa
   108241 tttttcaagg gcatgcaggg atttgtcgcg accgttgccg ctctgcttat agccgccaaa
   108301 cggcacggtc atatcgccgt cgttgtagtt attgacgaag acggaaccgg ctttcaggcg
   108361 gcggctcata cggtgcgcgc gggagaggtc gcgcgtccat accgccgcgc caaggccgta
   108421 ctggctgtcg ttggcaagct gtagcgcctg ttcttctgat gtgaaacgcg tgaccaccag
   108481 caccggaccg aaaatctctt cgcggcttaa ggacgcattc gggtctacat ccacaaagat
   108541 ggtcgggccg atggcggcag ccagcccggc gttacggcca tccagcagca gttgcccttt
   108601 gctttcgcct tcccgaataa agctatggac cgagtcggcg tgggcgcagt cgattaaagt
   108661 gcccatggtg gttgcggggt caagcggatg gcccggctgc cagttttgcg cctgctgttt
   108721 taacagggct aagaattcat cggcaatgct ctcttccagc aacaggcgtg ttccggcgat
   108781 gcacacctga ccctggttgt agaagatgcc tgctgcggtg gagctcgccg ctttttgcag
   108841 atccgaacaa tcggcgaaaa cgatgttggc gcttttgccg cccgcttcca gccagacgcg
   108901 tttcatgttg ctctcgcccg catctttcag cagctgtttc ccggtacggg ttgaaccggt
   108961 aaaggcaatg gcgtcgatat cgttatgacg cgacagcgcc tgcccggctt catgaccaaa
   109021 acccgtcacc acgttcaaca caccatccgg caagcctgct tctttcgcca gccccgcgag
   109081 acgaatcgca ctgagcggtg atttttcaga cggttttaga atcacgctgt ttcccgccgc
   109141 cagcgccggg ccgagtttcc agcaagtcag caacagcggg aagttccacg gcacgatggc
   109201 ggcaatcacg ccgaccggtt cacgcacgat catcgccagc tcatggctac tggtggtcgc
   109261 cacttcgcca tacactttgt cgatcgcttc ggcgtaccag cgaatggcgc gcgccgcgcc
   109321 gggaatatca tcacgcagac tgtgacgaat cggtttgccg gtgtcgagag tttccagcag
   109381 tgccagctct tcggcgtggg cttccattaa atcggcgagt ttattcagta ccgctttacg
   109441 tttagccgga gaagagagtg accagtcgcc gcgttcaaat acgccgcgtg ctgcgctcat
   109501 cgcacggtcg atatcgacgc tcttgccgcg ggcaattttc gccagcggtg cctgggtgac
   109561 cggatcaacg gtttcaaagg tttcattttc cgccgcagca gtatattcac cgttaataaa
   109621 taagcggttt tcaatggcga gacttaacgc tttatcctgc cagtaagcca gatgatgaaa
   109681 attcattatg actcctgttt cacgtctatc agatatatgc agtttgtgaa cgtcagggcg
   109741 cagagatccc cgggccgtat gcccgtttat ttctggcgac attctttata tacacttcat
   109801 ccttcaagcc gcctctgcct tggctgcgtt cgctcccccg aatcacttac ctgagtaagc
   109861 tcatcgggat tcgctcactt gccgccttga tgcaacttga atgattttgt gtatagattg
   109921 ccgggcgcat tgcgcttacc cggcttacag aacaaaaaga ttaaaacgtg gtgggcgtat
   109981 gggcgctgat aattcggcaa atacctgccg acgtattact gaaactgtgc ggtatgccgg
   110041 tattaatggc ataactttgc cccgcgacga ggtggtaatc ctgaccatta atcgtcagaa
   110101 caatttcacc ttccagtact gtgcctattt cctcaccctg atgcttaatt ctttccccag
   110161 tggttgtgcc cggctggtac gtttcaaaga tcatcgccag cgtgcgattc gggttaccgt
   110221 tatgaaccag cttcattgac acaccctgac tgcccatctc aattaagtcg tcctgattaa
   110281 tgacgacctg cggctcatca ggtttttccg gctcggaaaa gaattccgag agtgacagac
   110341 catacacctt cagcagcttt tgcagcgtac tgatggcagg gctgacttta tcttgttcta
   110401 tcgtactgat agcactgtga gtcagcccgg agagttcggc ggcacgacgt tgtgaaagcc
   110461 cctgctgctg gcggatttcc gacaagcgtt ttcctggcgc cagtccctca tcactcatat
   110521 ttgcatttcc ttaaactgta gtggtcagag tcgctgtttt tcagcgatat ggtgctgaca
   110581 agcggtgata aagccctcga acaatatacg cgaaagcgcg tactcgctac tgttccattc
   110641 cgggtgccac tgtacgccca gcgcaaaagg atgattgatg acgctaaccg cctcaactaa
   110701 gccatccggc gagcgagctt caacacgcaa ccgtgggcta acgaccttcg ccccttgtcc
   110761 atgtagagag tttacccaaa agttgctaca ttcaggtaac aacgcagaca gtaatccccc
   110821 ctcttcaacc tgaacttcat gcgacggtgc atattgctgt tccaccggca gttcgggatc
   110881 ttcccgatgt tccagcaatt caggctgttc gcacagcttg cgatgcaacg aaccaccggt
   110941 tgccaccact agttcttgta aaccccggca gatggcgaaa atgggaatgc gcctttcgag
   111001 tgcggcatta attatcgcca tgctcagaag atcacgcccg ggatcggcgt caggctcatc
   111061 gccgttttca ccatataggt gcggctgcac attgctggga ctaccaggaa gataaatgcc
   111121 atcgagtttc ggcaaaagtt gttcaagtaa cgacggttcc gccagcgcat gtggtagcgc
   111181 aattggcaag ccgcctgcgt ggatgatggc attcaggtac ttttcttgca gagtctgggt
   111241 cgcatgaccc ttaagcctgt tcctgcacat taagacaccg ataaccggat tgttcattat
   111301 attttccatg ctcaatctca caaagtggac taaattatcg ccattactgc gctttaaatg
   111361 acctttatgt tcaatatttt ttcaatctag cagtgagttt tcatttttgc aaactcaatt
   111421 taacatttga caaacattta gtttgcatac agattcgaat ggtggtcatt atattttacg
   111481 ctttgataac gagcggaaaa caaaccaaag gcgaagaatc atggaaacca atatcgttga
   111541 agtagagaac tttgttcagc agtcagaaga gaggcggggt agcgccttta cgcaggaagt
   111601 gaaacgctac ctggagcgct acccgaatac gcaatatgtt gatgttttac ttactgattt
   111661 gaatggttgc ttccgtggaa aacgtatccc tgtttcaagc ctgaagaagt tggagaaagg
   111721 gtgttatttc ccggcctcgg tgtttgcaat ggatattctg ggcaacgtag tagaagaagc
   111781 gggtctgggt caggaaatgg gcgagccgga tcgtacttgt gttcctgttc tcggttcctt
   111841 aaccccttct gccgccgatc cagagtttat cggtcagatg ctcctgacca tggtcgatga
   111901 agatggcgct ccctttgacg ttgagccgcg gaacgttctt aaccgcctgt ggcagcagct
   111961 acgccagcgc ggattgttcc cggttgtagc ggtagagctg gagttctatt tactggatcg
   112021 tcagcgcgac gctgaagggt atctgcaacc gccctgcgcg cccggcaccg atgaccgcaa
   112081 tacacaaagc caggtttact ccgttgataa cctcaaccac ttcgccgacg tgctcaatga
   112141 tattgatgaa ctggcgcagt tacagttaat tcccgccgac ggtgcggtcg ccgaagcctc
   112201 gccgggtcag tttgaaatca acctttacca taccgataac gtgctggaag cctgcgatga
   112261 tgcgctggcg ctaaaacgcc tggtgcgtct gatggcagaa aagcataaga tgcacgccac
   112321 ttttatggcg aagccgtatg aagagcacgc gggcagcggg atgcatatcc atatcagtat
   112381 gcaaaataat cgtggcgaga atgtgctttc tgacacagaa ggcgaagatt cgccgctgct
   112441 gaaaaagatg ctcgccggga tgattgacct gatgccgtcg tcgatggcgt tgctggcacc
   112501 aaacgtgaac tcgtatcgcc gcttccagcc gggaatgtat gtgccgacgc aggcgtcgtg
   112561 gggccataac aaccgcaccg tcgccctgcg tattccgtgc ggcgaccgtc ataatcaccg
   112621 cgtggaatat cgcgtggcgg gtgccgatgc caacccatat ctggtgatgg cagcgatttt
   112681 tgccggtatt ttgcatggcc ttgataacga gctgccgttg caggaagaag tcgaaggcaa
   112741 cgggctggaa caggaaggct tacccttccc gattcgccag agcgatgccc tgggtgagtt
   112801 tatcgagaat gatcacctgc gccgctattt aggcgaacgg ttttgccatg tgtatcacgc
   112861 ctgtaaaaat gatgagctgt tgcagtttga gcgcctcatc acagaaacag aaattgagtg
   112921 gatgttgaaa aacgcgtaat tccccatgcg gggttgtttc atcggcctga gcggagccag
   112981 gcctgacatt tgctggtaat ccagacattt cccggatgtc gcgttatgga gcgcaggcgg
   113041 caacgggctt ccgttaacga ttaagactgt gacgaggaaa tgagatgatg ccgatgatta
   113101 aatccccgca tggcgaggga ggctgcgtat gcgcccctcc tgctactgac tggactccgc
   113161 cccctttgct tcccttgcta aacaggtttg atttcaggtt gacacgaccg caaaccttat
   113221 tacgcagggg aggcagcaat tatggctatt aattcaccac tgaatattgc tgcgcaaccc
   113281 ggcaaaaccc gtctgcgaaa atcactgaaa ttgtggcagg tggtaatgat gggtctggcc
   113341 tatctcacgc cgatgaccgt atttgatact tttggtattg tctccggcat tagcgacggt
   113401 cacgttccgg cgtcctattt gctggcgctg gcgggcgtgc tgtttaccgc tatcagctac
   113461 ggcaaactgg ttcgccagtt tccggaggcc ggttcggcct atacctacgc gcaaaagtcg
   113521 attaatccgc acgtcggatt tatggtcggc tggtcatcac tgctggatta tctctttttg
   113581 ccaatgatca acgttttgtt ggcgaaaatc tatctctccg ccctcttccc ggaagtgccg
   113641 ccgtgggtgt gggtggtaac cttcgtcgcc attttaaccg ccgcgaatct gaagagcgtc
   113701 aacctggtcg ctaacttcaa taccctgttt gtactggtgc aaatctccat catggtggtg
   113761 tttatcttcc tggtagttca gggactgcat aaaggagaag gcgttggcac cgtctggtca
   113821 cttcagccgt ttatcagcga gaacgcgcac ctgatcccga ttattaccgg ggcgacgatt
   113881 gtctgtttct cgttcctcgg tttcgatgcg gtgaccacgc tttcggaaga gacgccggac
   113941 gccgcacgcg tgatcccgaa agccatcttc ctgacggcgg tatacggcgg cgttatcttt
   114001 atcgctgcgt cgttctttat gcagctgttc tttcctgata tcagccgttt taaagacccg
   114061 gacgccgcac tgcctgaaat tgcgctgtac gtcggcggca agctcttcca gtcgattttc
   114121 ctctgcacca cgtttgtgaa cacgttagcc tctggcctgg cttcacatgc cagcgtgtcg
   114181 cgtctgctgt atgtgatggg acgtgacaat gtgtttccgg agcgcgtgtt tggctatgtg
   114241 cacccaaaat ggcggacccc ggcactcaac gtcattatgg tcgggattgt cgcgttgtcg
   114301 gcgctgttct tcgatttagt taccgccaca gcgttaatta acttcggtgc tctggtggcg
   114361 tttaccttcg tgaatctgtc ggtgtttaat catttctggc ggcgtaaagg aatgaataaa
   114421 agctggaagg atcacttcca ctatttgctg atgccgctgg ttggcgcgct gacggtgggt
   114481 gtgctgtggg ttaacctaga gtcaacgtca ctgacactcg gtctggtatg ggcttcgtta
   114541 ggcggcacat atttgtggta tctgatccgc cgctatcgca aagtgccgct gtacgaaggt
   114601 gacagaacgc cggtgagcga aacgttatag ccggatgcgc ggcgcattat cctgcctgag
   114661 ggtaacgcgc cgcgccatta actataaaaa gatgtatgac ccggatcacg agtgctatca
   114721 tccgccctta tgcttagcgc attaacccca ggggcaaata atgaaccacg atatcccgct
   114781 aaaatatttt gatattgccg atgagtacgc gaccgaatgc gcagagcctg tcgcagaggc
   114841 tgaacgcaca ccgctggccc actacttcca gttactgctc acccgtttga tgaacaatga
   114901 agagatcagc gaagaagccc aacatgaaat ggctgctgaa gcggggatta atcccgtacg
   114961 cattgatgag atcgcagagt ttttgaatca atggggtaat gagtaatgat ttaattgcag
   115021 acgatcggtc agtatcgttg tccggataaa gcgttcacgc cgcatctggc taacaatgac
   115081 acgcactgca gcgtgcttta atgggtttct cacgatcgta ccgctctgct cctcgctaag
   115141 ttgttctgtg agttatagtt atagagaaat cacgggtacg aatgttgaac agatggcgac
   115201 gccacctgtt caacgaccaa ccacacggcc ctcattacga cccctgaaca caaacggtaa
   115261 tggcaaatgt cattaaatat tagtaagata gcctctttac ataaaatccc ttaatattat
   115321 gcgccaggta ttatcgtctc ttttggtgat tgctggactt gtgagtggtc aggcaatcgc
   115381 cgcgcctgaa tcccccccgc atgctgatat ccgcgacagc ggttttgtct attgcgtcag
   115441 cgggcaagtc aacaccttta acccatccaa agcgagcagt gggttaattg tcgataccct
   115501 tgccgcccag ttttatgatc gactgctgga tgtcgatccc tatacctatc gcctgatgcc
   115561 ggaacttgcc gaaagctggg aagtactcga caacggcgcg acctatcgct tccacctgcg
   115621 tcgcgatgtt ccgtttcaaa aaaccgactg gtttactccc actcgtaaaa tgaatgccga
   115681 cgatgtggtg tttaccttcc agcgaatttt tgaccgcaac aacccgtggc ataacgtcaa
   115741 cggcagcaac ttcccctact tcgacagcct gcaatttgcc gataacgtga aaagcgtccg
   115801 caaactggat aatcataccg ttgagttccg actcgcccag ccggatgctt cttttttgtg
   115861 gcacctcgca acccattatg cttcggtcat gtcggcagaa tatgcccgga agttagagaa
   115921 agaagatcgc caggagcaac tcgaccgtca accggtcggc accggtccgt atcagttgtc
   115981 ggaataccgc gccgggcaat ttattcgcct acaacgtcat gatgacttct ggcgcggtaa
   116041 accgttaatg ccgcaggtag tggtggattt aggctccggc ggcaccggac gtctgtcgaa
   116101 actcctgacc ggggaatgcg acgttctggc ctggcctgct gccagccagc tatccatttt
   116161 gcgtgacgac ccgcgcttgc gtttaacgct gcgtcctggg atgaacgtcg cctatctggc
   116221 atttaacacc gccaaaccgc cgctaaataa tcccgctgtc cgccatgcac tggcactggc
   116281 gattaataac cagcgcctga tgcaatccat ctattatggt acggctgaaa cggcggcctc
   116341 tattttaccg cgcgcctcgt gggcctatga caacgaggct aaaattactg aatacaatcc
   116401 ggcgaaatcg cgcgaacagt tgaaggcgtt ggggctggaa aacttaacgc tgaaactgtg
   116461 ggtgcccaca cgttcgcagg cgtggaaccc cagtccactg aaaactgccg aactgattca
   116521 ggcggatatg gcgcaggttg gcgtaaaagt ggtgattgtg ccggtagaag gtcgctttca
   116581 ggaggcgcgg ttgatggata tgagccatga tctgacgtta tccggttggg cgacggacag
   116641 taacgacccg gacagtttct tccgtccgtt actgagctgc gcggcaattc attcacagac
   116701 caacctcgcc cactggtgcg atccgaaatt cgacagcgtg ttgcgtaagg cgctctcctc
   116761 gcagcagctg gcagcgcgta ttgaagccta tgacgaagcg cagagtattc tggcgcagga
   116821 attacctatt ctgccgctgg cgtcgtcatt gcgtttgcag gcctatcggt acgatatcaa
   116881 aggtctggta cttagcccgt ttggtaacgc ctcctttgct ggggtgtatc gcgagaaaca
   116941 ggatgaggtg aaaaaaccat gattatcttc accttacgcc gcattttgtt attgattgtc
   117001 accttgttcc tgctgacctt cgttggcttc agtttgagct atttcactcc acacgcgccg
   117061 ttacaaggcg cgtcactgtg gaatgcctgg gtgttctggt ttaacggcct gatccactgg
   117121 gattttggtg tttccagcat taacggtcaa ccgattgccg aacagttaaa agaggtcttc
   117181 cctgcgacga tggaactgtg catcctcgcc ttcggttttg cgctgattgt tggcatcccc
   117241 gtggggatga ttgccgggat tacgcgccat aagtggcagg ataacttgat caacgccatc
   117301 gccttgctgg gcttttcaat cccggtgttc tggctggcgc ttctgttgac gctgttttgt
   117361 tcactcacgc taggttggtt gcccgtttca gggcgtttcg atctgctgta cgaagtgaaa
   117421 ccgattaccg ggtttgcgtt gattgatgcc tggctttcgg actcaccatg gcgggatgaa
   117481 atgatcatga gcgcaatccg ccatatgata ttgcccgtga tcaccctgtc ggttgcgcca
   117541 acaactgaag tgatccgact gatgcgtatc agcactatcg aagtgtatga ccagaactat
   117601 gttaaagcgg cggctacccg cggtttgtcg cgctttacta ttttgcgtcg ccacgttctg
   117661 cataacgcgt taccgccggt tattcctcgc ctgggcttac agttttccac catgctgacg
   117721 ctggcgatga tcaccgaaat ggtttttagc tggccgggtt tagggcgctg gttaattaac
   117781 gccatccgcc agcaggatta cgcagccatt tccgccggag tgatggtgtg tggctcactg
   117841 gtcattattg ttaacgtgat ttctgatatt ttgggtgcca tggctaaccc tctgaaacat
   117901 aaggaatggt atgccttacg atagcgtata cagcgaaaag cgcccgccgg gcacgctgcg
   117961 taccgcctgg cgcaaatttt atagtgacgc ctctgcgatg gtcggcctgt acggctgcgc
   118021 ggggctggct gtactgtgta tttttggcgg ctggtttgcg ccttacggca tcgaccagca
   118081 atttctcggt taccaattat tgccgccgtc atggtcgcgc tatggcgaag tttctttctt
   118141 cctggggact gacgacctcg gacgcgatgt tttaagccgt ttactgagcg gagctgcgcc
   118201 aaccgtgggt ggcgcatttg tggtgacgct tgccgcgacg atctgtggcc tggtactggg
   118261 caccttcgcc ggggcgacgc acggcctacg ctcagcggtg cttaaccata ttctggatac
   118321 cttactggcg atcccttcgc tgctgctggc aattatcgtt gtagcgtttg ccggaccgag
   118381 tttgtcccac gctatgtttg ctgtctggct ggcattgctg ccgcgtatgg tacgttcgat
   118441 ttacagcatg gtgcatgacg aactggaaaa agagtacgtt atcgccgccc gtctggatgg
   118501 cgcatcaacg ctgaatattc tctggtttgc tgtgatgcca aacatcaccg ccgggctggt
   118561 caccgagatc acccgcgcac tgtcgatggc aattctcgat atcgccgcgc tgggctttct
   118621 cgatctcggc gcacaactcc cctcgcctga atggggagcg atgctcggtg atgcgctgga
   118681 actgatttat gtcgccccgt ggactgtcat gctgccaggt gcggcaatta tgattagcgt
   118741 gttgttggtt aacctccttg gcgatggcgt tcgtcgtgcg attattgcgg gggtggaata
   118801 atgccattac tcgatattcg taacctgacc attgaattta aaaccggtga tgagtgggtt
   118861 aaagccgtcg accgggtaag tatgacctta accgaaggtg aaatccgcgg tcttgttggt
   118921 gaatccggtt ccggcaagag tttgattgcg aaagcgattt gtggggtgaa taaagataac
   118981 tggcgtgtta ctgctgaccg tatgcgtttt gatgatatcg atttgctgcg tctctccgca
   119041 cgcgaacggc gcaaactggt tggccataac gtgtcgatga ttttccagga accgcagtcg
   119101 tgtcttgacc cttcagaacg tgtgggtcgc cagttgatgc aaaacatccc agcctggacc
   119161 tataaaggcc gttggtggca gcgttttggc tggcgcaaac gccgtgccat tgaactgctg
   119221 caccgcgtgg ggattaaaga tcacaaagat gcgatgcgca gttttcccta tgagttgacc
   119281 gaaggtgaat gtcagaaagt gatgatagcc attgcactgg cgaatcaacc gcgtctgctg
   119341 attgctgacg aaccgaccaa ctcaatggag ccaacaaccc aggcgcaaat ctttcgcctg
   119401 ctgacgcgtc tcaaccaaaa cagcaatacc actattttgc ttatcagcca tgacttacaa
   119461 atgcttagcc aatgggcgga taaaattaac gtgctttact gcggtcaaac agtggaaacc
   119521 gcgccgagta aggagttggt gacgatgcca catcatcctt atacccaggc gctgatccgc
   119581 gcgataccag acttcggcag cgcgatgccg cataaaagtc gcctcaatac gctgcccggc
   119641 gctatcccac tgctggaaca gttaccgatt ggttgtcgtc tggggccacg ttgcccgtat
   119701 gcacaacgag aatgcattgt gacgccacgt ttgacggggg cgaaaaatca tctctatgcc
   119761 tgtcatttcc cgctgaacat ggagaaagag tgagatgatc gaaacgctgc ttgaagtgcg
   119821 taatttaagt aaaacgttcc gctaccggac cggctggttt cgtcgtcaga ccgtagaagc
   119881 ggtaaaaccc ttgagcttta cgctacgtga aggccagaca ctggcgatta ttggcgagaa
   119941 tggttcgggt aaatccacgc tggcaaaaat gctggcggga atgatagagc ctaccagcgg
   120001 cgagttattg attgacgatc atccactgca ttttggcgat tattccttcc gcagtcagcg
   120061 tattcgcatg atttttcagg atccttcgac ctcgttgaat ccccgtcaac gtatctcgca
   120121 aattctggat tttccactgc gcctgaacac cgacctggaa ccggaacagc gtcgcaaaca
   120181 gattattgaa acgatgcgta tggttgggct gctaccggat cacgtcagtt actatccgca
   120241 tatgttggca cccggacaaa aacagcgtct gggtctggcg cgcgcgctga tattgcgccc
   120301 aaaagtcatt attgccgatg aagccctcgc ttcactggat atgtcgatgc gttcgcagtt
   120361 gattaatctg atgctggagt tacaggaaaa acagggcatt tcgtatattt atgttaccca
   120421 gcatatcgga atgatgaagc acattagcga ccaggtgctg gtgatgcatc agggcgaggt
   120481 tgtcgagcga ggcagcaccg cggatgtgct ggcatcgccg ctgcatgaac tcaccaaacg
   120541 gctgattgcc gggcactttg gcgaagcatt gacagcggat gcgtggcgta aagatcgcta
   120601 attttctgaa tcctccttcc agattatcca cctcattctg acaagagatt aaatcttgtc
   120661 agaatgaggt gatggataaa ataaaatcaa atgcgcgtga tttaagacgc aatctcactt
   120721 tgcaggaacg aaagctctgg cgatatcttc gcagccgacg ttttggtgat ttcaaatttc
   120781 gccgtcaaca tccggtgggg agctacattc tcgattttgc ttgctgctcg gcgcgcgtag
   120841 tcgttgagct ggatggtggg cagcatgatt tggcagttgc ctatgatacc aggcgcacta
   120901 gctggcttga gtcgcagagc tggaccgtgc tgcgtttctg gcataacgag attgattgta
   120961 atgaggaggc ggtgctggag attattctgc aggaactgaa ccgccggtca ccctcaccct
   121021 gaaagggcga gggggccgac cgagccgaat agctgttgtg gtgaaaacat ggagacagtg
   121081 ctggagaata ttcggcaagg tctgaaccat cccagccatc gccataaaag ggttaggggc
   121141 tgtatgagcc tgtttgttgc tggggtaaca atatttgcac aatatggtcc cctcgcccct
   121201 ctggggagag ggttagggta aggggaaaag cgcccaccct gccgcagcct gctccggtcg
   121261 gacctggcaa ctatagctac tcacagccag gttgattata ataaccgttt atctgttcgt
   121321 actgtttact aaaacgacga atcgcctgat tttcaggcac aacaagcatc aacaataagg
   121381 attaaagcta tgggttttct ttccggtaag cgcattctgg taaccggtgt tgccagcaaa
   121441 ctatccatcg cctacggtat cgctcaggcg atgcaccgcg aaggagctga actggcattc
   121501 acctaccaga acgacaaact gaaaggccgc gtagaagaat ttgccgctca attgggttct
   121561 gacatcgttc tgcagtgcga tgttgcagaa gatgccagca tcgacaccat gttcgctgaa
   121621 ctggggaaag tttggccgaa atttgacggt ttcgtacact ctattggttt tgcacctggc
   121681 gatcagctgg atggtgacta tgttaacgcc gttacccgtg aaggcttcaa aattgcccac
   121741 gacatcagct cctacagctt cgttgcaatg gcaaaagctt gccgctccat gctgaatccg
   121801 ggttctgccc tgctgaccct ttcctacctt ggcgctgagc gcgctatccc gaactacaac
   121861 gttatgggtc tggcaaaagc gtctctggaa gcgaacgtgc gctatatggc gaacgcgatg
   121921 ggtccggaag gtgtgcgtgt taacgccatc tctgctggtc cgatccgtac tctggcggcc
   121981 tccggtatca aagacttccg caaaatgctg gctcattgcg aagccgttac cccgattcgc
   122041 cgtaccgtta ctattgaaga tgtgggtaac tccgcggcat tcctgtgctc cgatctctct
   122101 gccggtatct ccggtgaagt ggtccacgtt gacggcggtt tcagcatcgc tgcaatgaac
   122161 gaactcgaat tgaaataatc attctgttgg taaatattgg gcggcgttct gccgcccgtt
   122221 atctctgtta tacctttctg atatttgtta tcgccgatcc gtctttctcc ccttcccgcc
   122281 ttgcgtcagg ataacgattt cctttacgac caaggagcgc ccatggaaca acgccacatc
   122341 accggcaaaa gccactggta tcatgaaacg caatccagta ctgcggagta tgacgttctg
   122401 cctctggtcc cggaagccgc aaaggtcagc gatccctttc tgctcgacgt gatccttgat
   122461 gaagaaacgc tggctccctt cctttcatgg ctggtccctg cgcgcgttct tgcagtggaa
   122521 ttgttccctg accagcttac cgtgacccgt tcacagacct tcaccgctta tgaacgcttg
   122581 tctacggccc tgacggttgc tcaggtttgc ggcgtccagc ggttatgtaa ctactattcg
   122641 gcgcgactta cgccgctccc cgggcctgat tcctccaggg aaagcaatca taggttggca
   122701 caaatcacgc aatatgcccg ccaactggct agctcgcctt ctattatcga caaccgatcg
   122761 cgccagcatc tgaatgacgt cggtcttact gccggggact gtgtgatcat taaccaaatc
   122821 attggtttta ttggctttca ggcgcggacc attgcgacat ttcaggctta tctcgggcac
   122881 ccggtacgct ggttacccgg tctggaaata caaaactacg ccgacgcgtc actgtttgct
   122941 gatgaatcaa tacgctggcg aagcagctat gaggtggaaa aactacctga agagtacaca
   123001 aaaagttcaa ctgcagaact ttgccaactg gccgaaacac tctctctcca ccctatttca
   123061 ctttcccttc tcgaaaggtt gttaaacagt acacgggtta atacacagcc ggataataag
   123121 cttgcggcgt tgttatgcgc gcggataaat ggcagtcctg cttgttttgc cgcctgtatg
   123181 gattcatcaa atgaatataa aaaaatcagc gcccttgtgc gcaagggcga aaatgaaatt
   123241 aaccaatggg ctgaccgtca ttctgttgag cacgctaccg ttcaggcgat acaatggctg
   123301 acccgagcac ccgatcgctt tagcgccgcc cagttcagcc ctttactcga acacgaaaaa
   123361 tcatcaacgc agattattaa tctgctggta tggagcgggc tgtgtggctg gataaatcgc
   123421 ttaaaaatcg cgttgggtga gacatattaa ccttgccgcg tcagacagat tcgcgtaaaa
   123481 ctgtcagccg ctctaatggc caccaaaata gacaattatg tttcaggaca acccgctgct
   123541 agcgcagctt aaacagcaac tgcattccca gacgccacgc gctgaagggg tggtaaaagc
   123601 cacagaaaaa ggctttggct tcctggaagt cgacgcgcaa aaaagttatt tcattccgcc
   123661 gccgcagatg aaaaaagtca tgcatggcga ccgaattatc gcggtgatcc acagtgaaaa
   123721 agaacgtgaa tccgcagagc cagaagaact ggttgaaccg ttcctgactc gtttcgtggg
   123781 taaagttcag ggcaaaaatg accgtctggc catcgttcct gatcatccac tcttaaaaga
   123841 cgccattcct tgccgcgcag cccgtggcct gaaccacgag tttaaagaag gcgactgggc
   123901 ggttgccgaa atgcgccgtc atccgctgaa aggcgatcgt tctttctatg ctgaactgac
   123961 acaatacatc acttttggtg acgaccactt tgtaccgtgg tgggttacac ttgcgcgcca
   124021 taatctggaa aaagaagcac cagacggcgt cgctaccgaa atgctcgatg aaggtctggt
   124081 tcgtgaagat ctgaccgcgc tggattttgt caccatcgac agtgccagca cagaagatat
   124141 ggatgacgcc cttttcgcta aggcgttgcc ggatgacaaa cttcagctga ttgtggcgat
   124201 tgccgatcca accgcgtgga ttgcagaagg cagcaagctg gacaaagccg cgaaaattcg
   124261 cgcattcacc aactatctgc ctggcttcaa catccctatg ctgcctcgcg agctttctga
   124321 cgatctctgc tcactgcgcg ccaatgaagt ccgcccggta ctggcatgcc gcatgacgct
   124381 ctccgctgat ggcaccattg aagataatat cgaattcttt gccgccacca tcgaatccaa
   124441 agcgaagctg gtgtatgacc aggtttctga ctggctggaa aataccggtg actggcagcc
   124501 tgaaagtgaa gcaattgccg aacaagtccg tttgttagcg caaatttgcc aacgccgcgg
   124561 cgagtggcgt cataaccacg cactggtgtt taaagatcgc ccggattacc gctttattct
   124621 cggtgaaaaa ggtgaagtgc tggatatcgt cgccgagccc cgtcgcattg ccaaccgtat
   124681 cgtcgaagaa gcgatgattg ccgctaacat ttgtgcggcc cgcgtactgc gcgataagct
   124741 cggttttggc atctataacg tgcatatggg ctttgatccg gcaaatgccg acgcgctggc
   124801 agcgttgctg aaaacgcacg gtctgcatgt cgatgccgaa gaagtgctca cgctggacgg
   124861 tttctgcaaa ctgcgtcgtg aactggacgc gcaaccaact ggtttcctcg acagccgcat
   124921 tcgtcgcttc cagtcatttg ctgaaattag cactgaaccc ggtcctcact ttggcctcgg
   124981 tctggaagca tacgccacct ggacttcgcc gatccgtaaa tatggcgaca tgatcaacca
   125041 ccgtctgctg aaagcggtta tcaaaggcga aactgcgacg cgcccacagg atgaaatcac
   125101 tgtccaaatg gccgagcgtc gccgtctcaa ccggatggca gaacgtgatg ttggcgactg
   125161 gttatacgca cgcttcctga aagacaaagc cgggaccgac acccgtttcg cggcggaaat
   125221 tgtcgatatc agccgtggcg gcatgcgtgt gcgtttggtt gataacggcg ctatcgcctt
   125281 tattccggca cctttcttac acgctgtgcg cgatgaactg gtttgcagcc aggaaaacgg
   125341 cactgtacaa attaaaggtg aaacggttta caaagtaact gacgttattg acgtcaccat
   125401 tgccgaagtc cgcatggaaa cccgcagcat tattgcgcgc ccggtcgcgt aatctccttt
   125461 cacggcccat tcctcatgga tgggccgttt atttccccgc tctaccttcg ttatatccag
   125521 gcagattttt taactgactt tcgtttgaaa actggcgttt ttccaaaact ggattacaaa
   125581 taaatatata aaaaaaacaa tgcgtttagc tgccgttaat ttcatgtaag tcatgttgtt
   125641 ccttttattt aaccgcggac tccgcagtta accggaggat atgcatcatg aaaaccgtta
   125701 gggagtccac aacgttgtac aactttctcg gatcgcacaa tccatactgg cggttgacgg
   125761 aaagcagcga tgttttgcgc ttttctacca ccgaaaccac agaacctgat cgtactttgc
   125821 agttatctgc cgaacaggct gctcgcatca gggaaatgac ggtcatcacc tccagcctga
   125881 tgatgagtct gaccgtcgat gagagcgatc tttctgtgca tctggtagga cgaaaaatca
   125941 ataaacggga atgggctggc aacgcgtctg catggcatga cacaccggca gttgctcgtg
   126001 atttatcaca cgggctttcc tttgctgagc aggtagtttc tgaagcacat tccgcaatag
   126061 tgattctcga cagccggggg aatatccaac gcttcaatcg gttatgtgaa gattaaactg
   126121 ggttgaaaga acacgacgtc attggccaaa gcgtgtttaa actgtttatg agccgtcgtg
   126181 aagctgcggc atccaggcgc aataaccgtg tattttttcg aagcggcaat gcatatgaag
   126241 tcgaactgtg gataccaaca cgtaaaggcc agcggctgtt tctgtttcgc aataaatttg
   126301 tccacagcgg cagtggcaaa aacgagattt ttttaatctg ttccggcacc gacattaccg
   126361 aagagcgccg cgctcaggag cgactgcgta ttctggcaaa taccgacagt atcaccggac
   126421 tgccgaatcg taacgcaatg caggatttaa tcgatcacgc tattaatcat gcagataaca
   126481 ataaagttgg ggttgtgtat cttgatttgg ataatttcaa aaaggtcaac gacgcctatg
   126541 ggcatttgtt tggtgaccag ttattacgcg acgtgtcatt ggctatttta agctgtctcg
   126601 aacatgacca ggtgttggcg cgtccaggtg gcgatgagtt tctggtactg gcatccaaca
   126661 cctcacaaag cgcgctggaa gcaatggcat cacgaatttt gacccgctta cggctcccct
   126721 ttcgcattgg tttaattgaa gtttatacca gctgttcagt aggtattgca ctctctcccg
   126781 aacatggttc agacagcacg gctattattc gtcacgccga cacagcaatg tacacagcga
   126841 aggaaggcgg acgaggacaa ttttgcgttt ttaccccaga aatgaatcaa cgggtatttg
   126901 aatatctctg gctggatacc aacttgcgta aagcactgga aaacgatcag ttggttattc
   126961 actatcaacc gaaaatcacc tggcgtggcg aagtgcgcag tctggaagca ctagtacgtt
   127021 ggcagtcacc tgaacgtggg ttgattccac cgttggactt catttcctac gccgaagagt
   127081 cagggctaat tgtgccttta ggccgttggg tgattctcga tgtcgtacgc caggtggcga
   127141 agtggcggga taaaggtatt aacctgcgag tggcggtaaa tatttctgca cgtcagctcg
   127201 ccgatcaaac cattttcacc gccctgaaac aggttctcca ggaactcaat tttgaatact
   127261 gccctataga tgttgaactg acagagagtt gtctgattga gaatgatgaa ctggcactgt
   127321 ctgttattca acaatttagc caactaggtg cgcaagtgca tctggatgat tttggtaccg
   127381 gctactcttc actttcgcaa ctggcgcgct ttccgatcga tgccatcaaa cttgaccagg
   127441 tttttgttcg agatattcac aaacaacctg tctcgcagtc actggtccgg gcgatcgtcg
   127501 ctgtggccca ggcattgaat cttcaggtga tcgccgaagg tgtagagagt gcaaaggaag
   127561 atgctttttt aaccaagaac gggatcaatg agcggcaagg atttttgttt gccaaaccga
   127621 tgcccgccgt cgccttcgaa cgctggtata aacgctatct gaagcgcgca taagtagatg
   127681 aatatatata cttagcgcta tatcccgtca ttatatatcg tattcctgga tgataattag
   127741 ccgttgctgt taaacacttc tcttttagcg gcaatttttg cattattcat tttccacttt
   127801 tgctataagg atgtccttga tgtctgaatt tgatgcccag cgcgttgccg aacgtattga
   127861 tattgtgtta gatatccttg tggcaggcga ttaccactct gcgatccata atctcgagat
   127921 acttaaagca gaactcttgc gccaggttgc tgaatctacg ccagacatcc cgaaggcacc
   127981 gtgggagatt taacaatcgt gccttcaggt tttcctattt gaggatgccc ggcgcgtcag
   128041 tattttttta tttagtatta taacgttata agaattacag cgatgaattc ccgacaacaa
   128101 actattctac agatggtcat tgaccagggt caggttagcg taaccgatct ggcaaaagcc
   128161 actggagttt ctgaagttac cattcgccag gatctcaaca ccctcgaaaa actgagttac
   128221 ctccgccgtg cacatggctt tgcagtttcg cttgatagtg atgacgtcga aacccgtatg
   128281 atgagcaact atacgctgaa gcgtgaactc gccgagtttg ccgcgtcact ggttcaaccg
   128341 ggcgaaacca tctttatcga aaatggtagc agcaatgccc tgctcgcccg gactctgggc
   128401 gaacagaaga aaaatgtcac tatcatcaca gtcagcagct acattgcgca tttgctgaaa
   128461 gacgcgcctt gtgaagtcat tttgctcggt ggcgtgtacc agaaaaaaag cgaaagtatg
   128521 gttggccctt tgacacgcca gtgcatccag caggtacatt tcagcaaagc atttattggt
   128581 attgatggct ggcaacctga aactggattc accggtcgcg atatgatgcg taccgatgtg
   128641 gtcaatgccg tgctggaaaa agagtgcgaa gcgatagtcc tgactgacag ctcgaaattt
   128701 ggtgctgtac attcatactc catcggtccc gttgagcgat tcaatcgcgt gattaccgat
   128761 tcgaaaatac gcgccagcga tctgatgcat cttgagcaca gtaaactcac cgttcacgtc
   128821 gttgacattt aaccatttcc cttctctacg gatgatttgc agtttggcaa atcatccgct
   128881 ctaagatgat tcctggttga taattaagac tatttacctg ttattaacac tctcaagata
   128941 taaaattatt atcagcgata taacaggaag tcattatcac ctgcgtgata taaccctgcg
   129001 cgcgagcaga tttcacggaa taatttcacc aggcttattc ttagctatta tagttataga
   129061 gagcttactt ccgtgaatca taaattcagg agagagtatt atgtttgtaa cgagcaaaaa
   129121 aatgaccgcg gctgttctgg caattaccct ggcaatgtct ctgagtgcct gttctaactg
   129181 gtctaaacgg gaccgcaaca ccgcaatcgg cgcgggtgca ggggcattag gcggtgcagt
   129241 actgaccgat ggcagtacgt tgggtacatt aggtggtgca gctgtcggtg gtgttattgg
   129301 tcaccaggtc ggtaaataat cgtaaataaa tgtcgattat cgactcgcaa ttataatacg
   129361 cttgattctg ttcagtacga aataactgaa taatacacac agtaaaatat tcgaaagcca
   129421 cggatatatc cgtggctttt tatgttaacc acctgcgagt tttaccttca tccctttcgc
   129481 ttcaagtagt gattttaata aatcacgctt atcgccctgg atttcaataa ctccatcttt
   129541 aactgctccg ccgcagccgc attttttctt cagttccgct gcaagttttg tcagttcggc
   129601 atcatcgaga tcgacaccgg taatcaggca aacgccctta cctttacgtc cactggtctg
   129661 acgctgaata cgcaccacac cgtcgccttt agggcgtaca ggggccgctt tgggctcatc
   129721 aatacgtccg gtttccgttg agtagaccag acggctgttg gaatcactca tcatgcactc
   129781 cgctgtaaag aggcgttgat cgctttcagc gtctgcgctg gatctaccga ttgcgttacc
   129841 gggcgaccaa tcaccatata atcaacacca gccgacaacg cctgttctgg cgtcataatg
   129901 cggcgctggt caccagcttc actcccctgc ggacgaatgc ccggcgtaac cagtttgaac
   129961 tcctgaccga atacctgttt aaagcgcaca gcttcctgag cagaacacac cacaccatca
   130021 aggccacatt tttgcgtcag tgccgccaga cgttctgcat aatctgcagg tgacagtgtc
   130081 atgccaagat cgaccaggtc gctggcttcc atgctggtca acactgtcac agcaatcaaa
   130141 agcggtgcat ctttgccaaa cggaaccagt gcctcacgcg ctgcggtcat catacgcgcc
   130201 ccaccagagg catgaacatt caccatccac acgcctaagt cagctgcagc agcgacagcg
   130261 tgcgcagcag tgttaggaat atcgtggaat ttcaggtcaa gaaagatatc aaaaccacgc
   130321 tgttgaagtt cgcgcacaaa ctgtggccca aacaatgtaa acatctcttt gccgaccttc
   130381 agacgacaat cgcgtgggtc gatcttgtcg acaaaggaca gcgcgtcatc acgattatga
   130441 taatcaaggg caacaaccac aggagaattc gtaacagcgc gggaagaaga tgaagcagtt
   130501 aacgtcatga ccagaccttc ttgatgatgg gcaccggaag tggcgcgaaa caggtaaacg
   130561 gcgagcattc tacccgtgga aataaaaaat taacagttgc gatttttccc ctggcaggct
   130621 ttttctgcgc cgtcgttgcc ggagcacaat ggaacatcaa taactagtat gttgtaacta
   130681 aagtcggatt tttttaaaaa ttacaggcca tcaagaccgc gaatcggttt aatggttgac
   130741 caggcccgac aagacggaca atgccagtag agggtgtatg cggtaaaacc acatttctgg
   130801 cagcgataac gaggcttact acgtaccttt tcgccaacca tgtcacgcag caccatcagg
   130861 ctctctttgg cacgcccttc ttccgcttca tttaagtggt aatccattaa cttatggaac
   130921 acacgcatgg tcggatgacg ctgaagctgg cgcgtaatat agacctgtgc ggcctcacta
   130981 ccgtcgcgcg cttcgatgat atcagcaagc atcaattcag catcggcacc ggtgttctct
   131041 tccaccgcgc gttgcaggaa ttctgcccat tcggcagttt tacccaactg ctgatagcag
   131101 gtttgcaaca tttccagcgt ttcgctgacc agttctctgt cctgggatat cacgcgttgc
   131161 aaactttcga cggctttggc gtattctcct ttcgccataa acacgcgtcc catcatgatg
   131221 gatacgcggg cgctgttttt atctgccgcc gccccttttt ttagcaaggt catggcacga
   131281 tcgagatcgt cgctggccat atgctgcagg gctaactcac agtagaaatg ggcaatttcg
   131341 acgcgctgtt tatctttacc cagcttcacc aggcgttcgg caacatcaat tgctttctgc
   131401 cactcactgg tagcctggta gatttgtagc aactgttgca gcgcgccaat gcggaagtca
   131461 gtttcatcgg tcagctgatt gaacatgtct tccgcgcggt cgtataaccc ggcggccatg
   131521 taatcacgcc ctagttgttg aatcgccaac agacgctgtt cataggtcag cgaggcgctt
   131581 tccattaggg tctgatggat gcgaatagcg cgatcaactt cgccacgcga acggaacagg
   131641 tttccgagcg taaggtgggc ttcaacggtg cctgtatcct ctttaagcat atcgagaaac
   131701 aggtctaccg ctttatcctg ttgattacta agtaggaagt taacccccgc tacgtaatca
   131761 cgcgacaagc ggttggcttc atcttgcttg ttttgttgcg cacttctgcg gcccatatac
   131821 cagccatagg cagcggctac aggcaaaagc agaaacaaca actccagcat agaaagttat
   131881 tccttcgccg ctgacgagtg cggcactaca gccacgtcag tcgcgggtga aagctggttt
   131941 tccagtcgct ttattttacg ttcagcgcgc gccagggaaa cacgaactcg cagccagaac
   132001 aggccacaaa tcaaccaacc gatagcaaac cccgcagcaa acaataccgc cagcaatgtg
   132061 gaaatacggt actccccttg cgctaacaga taattaaacg tcacctgttg atcgttctgc
   132121 gcacccaacg tgaccgaaat cacgaagatc gctaacacca gtaagaaaat gagtaaatat
   132181 ttcacattac ttcccgttat gtggttagag cgaataaatt atggtcaact taccgcaata
   132241 gccctttaaa gtaccatttt cgcgccgtct gcgaaatggc atccacgcgc attgttgatg
   132301 atttaagggc taaattcgga aaatcaacca gcgttaactt tcttgttctc gttgcgctat
   132361 ttcgcgattt tcttccgcag gtggtgttaa tggcccacaa attcgttgcg caagccacgt
   132421 tgccaccgcc accagcgccc acgaaatcaa cgtagctact accagatcgc gtggccaatg
   132481 catcccgagc agcaggcggc ttcccatgac tcccgttgcc cagaccagca agatagcaat
   132541 ggttaacgtt cgccgacgcg gccacagcaa accaacggcc agcagtgccc aactggcagc
   132601 aaacatcgtg tgaccggaag gaaaggcaaa ccccgtctct ttctgccagt gtgaacgcaa
   132661 atattgtggg atatttttct cttcagccaa ctgttctttc actaaatttc cgcgttctgc
   132721 tcgctttaaa gtgtagaact catcaaccgg aatatgatgt gttttttcca gccagataac
   132781 aaaaggtcgt ggttcctgga ctttgtcttt gatccaggat ttaacgcctt gtcccacaag
   132841 gattgcggcc gccagaatgg caaataatac aaaggcagcc ttaatgcgaa aacgcagaca
   132901 ccagagaaac cagccgaata aaatcaaatg tgtaatgacg ccccagggct gggtgacagt
   132961 ttcagtaacc caaaaagccg cttttagtag ccaactttgt tctccaggtt gccaacgcca
   133021 gccagaaatc catacggcta ctggcatgac aagcaatagt gcaactccca ctgcggtacg
   133081 tctggcaatc gaacgcatgg cctctccttt ttgataagtc ccacaatcat aactgaaaac
   133141 gccagttcca ggaaaaattg acagatttgt gccattccgt gaacgatcga cgcgtcgtga
   133201 ttaggtgaac cccttctcgt tatggcaaaa taagccaata cagaaccagc attatctgga
   133261 gaatttcatg cagcttaaac gtgtggcaga agccaaactg ccaaccccat ggggcgattt
   133321 cctgatggtg ggatttgaag aactggcaac cggacacgat catgtcgcgc tagtctatgg
   133381 cgatatttcc gggcataccc cggtacttgc gcgcgtccat tccgaatgtc tgaccggtga
   133441 cgccctgttc agcttgcgct gcgattgtgg cttccagctc gaagcggcat tgacgcaaat
   133501 tgccgaggaa ggccgtggta ttttgctgta tcaccgtcag gaaggtcgta acattggtct
   133561 gctgaataaa atccgcgctt acgcactgca ggatcaaggt tacgataccg tagaggctaa
   133621 ccaccagtta ggcttcgccg ctgatgagcg cgacttcact ctttgcgctg atatgttcaa
   133681 actccttggc gtcaatgaag tccgcttgtt aaccaataac ccgaaaaaag tcgaaattct
   133741 gaccgaagca gggattaata ttgttgaacg cgtaccattg attgtaggtc gtaaccccaa
   133801 taacgaacat tatctcgata ccaaagccga gaaaatgggt catttgctga acaaataacc
   133861 ctctcgcatt gtggaattaa tttgcttgcc ggaagcaaaa taaccggcaa gcaaatactt
   133921 gttacttcag catattacga atgacataat gcaaaatgcc gtcgttctgg tagtaggtca
   133981 actccgtcgc ggtgtcgata cgacaacggc agggtacgac ctcctggcta ccatccgcac
   134041 gcgtaagcgt caccggaacc gtcgcaccgg gttgtagatt ttgcagatcg ccaatatcaa
   134101 tcttctcttc cccggttagt cctaacgttt tacgcgttac gccttgcgga aattccagcg
   134161 gcaggatgcc catgccaatt aaattcgaac ggtgaattcg ttcaaacgat tcggcaatca
   134221 ccacacgaat tccaagcaga cgaggacctt ttgccgccca gtcacggctt gagcctgaac
   134281 catactcttt cccggcaatc accgccagcg gcgtttgttc ctgcttatag cgcatcgcag
   134341 catcataaat agagactacg tcgctgtcag gtaaatgccg cgtcatcccc ccttcaacgc
   134401 caggcaccat ttcattacgg atgcgaatat tggcgaaggt gccgcgcatc atcacttcat
   134461 ggttaccacg ccgcgaaccg taggagttaa agtcttttcg ctcaacaccc cgaccttgta
   134521 gatatcgacc cgctgggctg tcgggcttaa tactgcccgc cggagagata tggtcagtgg
   134581 tgactgaatc ccccagcatt gcgaggatcc gcgcaccgtg aatatcttcc actggtgctg
   134641 gtgttgcctg catttcatca aagaaaggcg ataagcgaat ataggttgag tcctcctgcc
   134701 aaccgtaggt atcggatcgt gtgacgttaa tttccttcca ctctgctgtg ccttcaaaaa
   134761 cttctgcgta ctctttgcgg aacatttctg tggagacttg ttctaccgca cgggcaattt
   134821 cttgtgccga tggccagata tctttcagat ataccggatc gcctttacga tcatggccga
   134881 taggctcaga agccaggttg atattcatat ttcccgccag cgcataggca accaccagcg
   134941 gcggcgaggc cagccagttg gttttaacca gcggatggat acggccttca aagttacggt
   135001 tgccggacag cactgcaccg acggttaaat cgcctttttt gattgccgtt tcgataggat
   135061 cgggcagcgg cccagagtta ccaatacagg tggtacaacc gtatcccaca aggttaaacc
   135121 ccagttcgtc gagatacggt gtcaatttcg cttttgccag ataatcagaa acaactttcg
   135181 agcctggtgc cagcgacgct ttgacccacg gttgccgctt gaggccaaga gttacggctt
   135241 ttttcgccag caagcctgca gccatcagca cacttgggtt agaggtgttg gtgcacgagg
   135301 ttatcgcagc aatgaccaca gcgccatcag gtaactgata ctgatgtccg ttcataacat
   135361 aatcgaccgg ctggcgatct ttatgcgtgg cattcacttc cagttcgtta ctggcggcaa
   135421 atgcttttgg tacatcgggc agtgcaacgc gatcctgtgg gcgttttggc cctgccaggc
   135481 tcgcttcaac gtcattcata tccagttcta acgtactggt aaaaatcggt tcatcgcccg
   135541 ggttacgcca catgccctgc gctttggcat atttttcgac caactcaacc tgatcttcgc
   135601 tgcgcccgct taaacgcatg taatcgaggg ttacagcatc gattgggaag aagccacagg
   135661 tggcaccata ttctggcgac atattggcaa tggtggcgcg atccgccagc ggtagtgaat
   135721 ccagaccatc accataaaat tcgacgaatt tccccaccac gccatgtttg cgcagcattt
   135781 gggtaacagt gagaaccagg tctgtggcgg taataccttc acgtaattta ccggtaagtt
   135841 taaagcccac tacgtccggg ataagcatgg aaaccggctg gcctaacatt gcggcttct
//
LOCUS       DAZGSP010000002        95821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZGSP010000002 DAZGSP010000000
VERSION     DAZGSP010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 95821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.002"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            50..568
                     /gene="yfgH"
                     /locus_tag="ACHK74_000130"
     CDS             50..568
                     /gene="yfgH"
                     /locus_tag="ACHK74_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP0219017.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAVGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            584..1123
                     /gene="yfgI"
                     /locus_tag="ACHK74_000131"
     CDS             584..1123
                     /gene="yfgI"
                     /locus_tag="ACHK74_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFP0219018.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1218..2795)
                     /gene="guaA"
                     /locus_tag="ACHK74_000132"
     CDS             complement(1218..2795)
                     /gene="guaA"
                     /locus_tag="ACHK74_000132"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP0219019.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2864..4330)
                     /gene="guaB"
                     /locus_tag="ACHK74_000133"
     CDS             complement(2864..4330)
                     /gene="guaB"
                     /locus_tag="ACHK74_000133"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFP0219020.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4492..5862
                     /gene="xseA"
                     /locus_tag="ACHK74_000134"
     CDS             4492..5862
                     /gene="xseA"
                     /locus_tag="ACHK74_000134"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFP0219021.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5859..6074)
                     /gene="yfgJ"
                     /locus_tag="ACHK74_000135"
     CDS             complement(5859..6074)
                     /gene="yfgJ"
                     /locus_tag="ACHK74_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP0219022.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6143..7615)
                     /gene="der"
                     /locus_tag="ACHK74_000136"
     CDS             complement(6143..7615)
                     /gene="der"
                     /locus_tag="ACHK74_000136"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFP0219023.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7733..8911)
                     /gene="bamB"
                     /locus_tag="ACHK74_000137"
     CDS             complement(7733..8911)
                     /gene="bamB"
                     /locus_tag="ACHK74_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFP0219024.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGLTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8922..9542)
                     /gene="yfgM"
                     /locus_tag="ACHK74_000138"
     CDS             complement(8922..9542)
                     /gene="yfgM"
                     /locus_tag="ACHK74_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFP0219025.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9560..10834)
                     /gene="hisS"
                     /locus_tag="ACHK74_000139"
     CDS             complement(9560..10834)
                     /gene="hisS"
                     /locus_tag="ACHK74_000139"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFP0219026.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10945..12063)
                     /gene="ispG"
                     /locus_tag="ACHK74_000140"
                     /gene_synonym="gcpE"
     CDS             complement(10945..12063)
                     /gene="ispG"
                     /locus_tag="ACHK74_000140"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFP0219027.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12090..13103)
                     /gene="rodZ"
                     /locus_tag="ACHK74_000141"
     CDS             complement(12090..13103)
                     /gene="rodZ"
                     /locus_tag="ACHK74_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFP0219028.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13388..14542)
                     /gene="rlmN"
                     /locus_tag="ACHK74_000142"
     CDS             complement(13388..14542)
                     /gene="rlmN"
                     /locus_tag="ACHK74_000142"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFP0219029.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14692..15123)
                     /gene="ndk"
                     /locus_tag="ACHK74_000143"
     CDS             complement(14692..15123)
                     /gene="ndk"
                     /locus_tag="ACHK74_000143"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFP0219030.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15272..17584)
                     /gene="pbpC"
                     /locus_tag="ACHK74_000144"
     CDS             complement(15272..17584)
                     /gene="pbpC"
                     /locus_tag="ACHK74_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFP0219031.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAKLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17585..22546)
                     /gene="yfhM"
                     /locus_tag="ACHK74_000145"
     CDS             complement(17585..22546)
                     /gene="yfhM"
                     /locus_tag="ACHK74_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFP0219032.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLLENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLYLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22753..23598
                     /gene="sseA"
                     /locus_tag="ACHK74_000146"
     CDS             22753..23598
                     /gene="sseA"
                     /locus_tag="ACHK74_000146"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFP0219033.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23934..24059
                     /gene="timP"
                     /locus_tag="ACHK74_000147"
     CDS             23934..24059
                     /gene="timP"
                     /locus_tag="ACHK74_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFP0219034.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24091..24867)
                     /gene="sseB"
                     /locus_tag="ACHK74_000148"
     CDS             complement(24091..24867)
                     /gene="sseB"
                     /locus_tag="ACHK74_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFP0219035.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKSVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(25009..26292)
                     /gene="pepB"
                     /locus_tag="ACHK74_000149"
     CDS             complement(25009..26292)
                     /gene="pepB"
                     /locus_tag="ACHK74_000149"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFP0219036.1"
                     /translation="MTEAMNITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26470..26670)
                     /gene="iscX"
                     /locus_tag="ACHK74_000150"
     CDS             complement(26470..26670)
                     /gene="iscX"
                     /locus_tag="ACHK74_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFP0219037.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26682..27017)
                     /gene="fdx"
                     /locus_tag="ACHK74_000151"
     CDS             complement(26682..27017)
                     /gene="fdx"
                     /locus_tag="ACHK74_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFP0219038.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27019..28869)
                     /gene="hscA"
                     /locus_tag="ACHK74_000152"
     CDS             complement(27019..28869)
                     /gene="hscA"
                     /locus_tag="ACHK74_000152"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFP0219039.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28886..29401)
                     /gene="hscB"
                     /locus_tag="ACHK74_000153"
     CDS             complement(28886..29401)
                     /gene="hscB"
                     /locus_tag="ACHK74_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFP0219040.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29497..29820)
                     /gene="iscA"
                     /locus_tag="ACHK74_000154"
     CDS             complement(29497..29820)
                     /gene="iscA"
                     /locus_tag="ACHK74_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFP0219041.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29837..30223)
                     /gene="iscU"
                     /locus_tag="ACHK74_000155"
     CDS             complement(29837..30223)
                     /gene="iscU"
                     /locus_tag="ACHK74_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFP0219042.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30251..31465)
                     /gene="iscS"
                     /locus_tag="ACHK74_000156"
     CDS             complement(30251..31465)
                     /gene="iscS"
                     /locus_tag="ACHK74_000156"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFP0219043.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31577..32065)
                     /gene="iscR"
                     /locus_tag="ACHK74_000157"
     CDS             complement(31577..32065)
                     /gene="iscR"
                     /locus_tag="ACHK74_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFP0219044.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32345..33085)
                     /gene="trmJ"
                     /locus_tag="ACHK74_000158"
     CDS             complement(32345..33085)
                     /gene="trmJ"
                     /locus_tag="ACHK74_000158"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFP0219045.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            33204..34007
                     /gene="suhB"
                     /locus_tag="ACHK74_000159"
     CDS             33204..34007
                     /gene="suhB"
                     /locus_tag="ACHK74_000159"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFP0219046.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34152..35006
                     /gene="yfhR"
                     /locus_tag="ACHK74_000160"
     CDS             34152..35006
                     /gene="yfhR"
                     /locus_tag="ACHK74_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0219047.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGRGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35197..36477
                     /gene="csiE"
                     /locus_tag="ACHK74_000161"
     CDS             35197..36477
                     /gene="csiE"
                     /locus_tag="ACHK74_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFP0219048.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSFF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36469..37608)
                     /gene="hcaT"
                     /locus_tag="ACHK74_000162"
     CDS             complement(36469..37608)
                     /gene="hcaT"
                     /locus_tag="ACHK74_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFP0219049.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37768..38658)
                     /gene="hcaR"
                     /locus_tag="ACHK74_000163"
     CDS             complement(37768..38658)
                     /gene="hcaR"
                     /locus_tag="ACHK74_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFP0219050.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPVYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38794..40155
                     /gene="hcaE"
                     /locus_tag="ACHK74_000164"
     CDS             38794..40155
                     /gene="hcaE"
                     /locus_tag="ACHK74_000164"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFP0219051.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40152..40670
                     /gene="hcaF"
                     /locus_tag="ACHK74_000165"
     CDS             40152..40670
                     /gene="hcaF"
                     /locus_tag="ACHK74_000165"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFP0219052.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40670..40990
                     /gene="hcaC"
                     /locus_tag="ACHK74_000166"
     CDS             40670..40990
                     /gene="hcaC"
                     /locus_tag="ACHK74_000166"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFP0219053.1"
                     /translation="MNRIYACPVADAPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            40987..41799
                     /gene="hcaB"
                     /locus_tag="ACHK74_000167"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             40987..41799
                     /gene="hcaB"
                     /locus_tag="ACHK74_000167"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFP0219054.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNVSLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41809..43011
                     /gene="hcaD"
                     /locus_tag="ACHK74_000168"
     CDS             41809..43011
                     /gene="hcaD"
                     /locus_tag="ACHK74_000168"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFP0219055.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43108..43530
                     /gene="yphA"
                     /locus_tag="ACHK74_000169"
     CDS             43108..43530
                     /gene="yphA"
                     /locus_tag="ACHK74_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP0219056.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43578..44450)
                     /gene="yphB"
                     /locus_tag="ACHK74_000170"
     CDS             complement(43578..44450)
                     /gene="yphB"
                     /locus_tag="ACHK74_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFP0219057.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44462..45556)
                     /gene="yphC"
                     /locus_tag="ACHK74_000171"
     CDS             complement(44462..45556)
                     /gene="yphC"
                     /locus_tag="ACHK74_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP0219058.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDKVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINSPD"
     gene            complement(45589..46587)
                     /gene="yphD"
                     /locus_tag="ACHK74_000172"
     CDS             complement(45589..46587)
                     /gene="yphD"
                     /locus_tag="ACHK74_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0219059.1"
                     /translation="MSASSLSLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46612..48123)
                     /gene="yphE"
                     /locus_tag="ACHK74_000173"
     CDS             complement(46612..48123)
                     /gene="yphE"
                     /locus_tag="ACHK74_000173"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP0219060.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(48146..49129)
                     /gene="yphF"
                     /locus_tag="ACHK74_000174"
     CDS             complement(48146..49129)
                     /gene="yphF"
                     /locus_tag="ACHK74_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP0219061.1"
                     /translation="MPTKIRTTRNLLLMATLLGSALFARAAEKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGGDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVLAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49226..52507)
                     /gene="yphG"
                     /locus_tag="ACHK74_000175"
     CDS             complement(49226..52507)
                     /gene="yphG"
                     /locus_tag="ACHK74_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFP0219062.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVVLDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QRAKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDYQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52625..53818
                     /gene="yphH"
                     /locus_tag="ACHK74_000176"
     CDS             52625..53818
                     /gene="yphH"
                     /locus_tag="ACHK74_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP0219063.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPGRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(53882..55135)
                     /gene="glyA"
                     /locus_tag="ACHK74_000177"
     CDS             complement(53882..55135)
                     /gene="glyA"
                     /locus_tag="ACHK74_000177"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFP0219064.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55463..56653
                     /gene="hmpA"
                     /locus_tag="ACHK74_000178"
     CDS             55463..56653
                     /gene="hmpA"
                     /locus_tag="ACHK74_000178"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFP0219065.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56698..57036)
                     /gene="glnB"
                     /locus_tag="ACHK74_000179"
     CDS             complement(56698..57036)
                     /gene="glnB"
                     /locus_tag="ACHK74_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFP0219066.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57097..58431)
                     /gene="glrR"
                     /locus_tag="ACHK74_000180"
     CDS             complement(57097..58431)
                     /gene="glrR"
                     /locus_tag="ACHK74_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFP0219067.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58421..59134)
                     /gene="qseG"
                     /locus_tag="ACHK74_000181"
     CDS             complement(58421..59134)
                     /gene="qseG"
                     /locus_tag="ACHK74_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFP0219068.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTLAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PREGEKPAPSTHEVTPDEP"
     gene            complement(59298..60725)
                     /gene="qseE"
                     /locus_tag="ACHK74_000182"
     CDS             complement(59298..60725)
                     /gene="qseE"
                     /locus_tag="ACHK74_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFP0219069.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61301..65188)
                     /gene="purL"
                     /locus_tag="ACHK74_000183"
                     /gene_synonym="purI"
     CDS             complement(61301..65188)
                     /gene="purL"
                     /locus_tag="ACHK74_000183"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFP0219070.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIGSLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            65446..67002
                     /gene="mltF"
                     /locus_tag="ACHK74_000184"
     CDS             65446..67002
                     /gene="mltF"
                     /locus_tag="ACHK74_000184"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFP0219071.1"
                     /translation="MKKLKINYLFIGILALLLVVALWPSIPWFGKADNRIAAIQARGQ
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(66999..67502)
                     /gene="tadA"
                     /locus_tag="ACHK74_000185"
     CDS             complement(66999..67502)
                     /gene="tadA"
                     /locus_tag="ACHK74_000185"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFP0219072.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(67560..68195)
                     /gene="yfhb"
                     /locus_tag="ACHK74_000186"
     CDS             complement(67560..68195)
                     /gene="yfhb"
                     /locus_tag="ACHK74_000186"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFP0219073.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGGTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            68404..69252
                     /gene="yfhH"
                     /locus_tag="ACHK74_000187"
     CDS             68404..69252
                     /gene="yfhH"
                     /locus_tag="ACHK74_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP0219074.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGDKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            69308..69568
                     /gene="yfhL"
                     /locus_tag="ACHK74_000188"
     CDS             69308..69568
                     /gene="yfhL"
                     /locus_tag="ACHK74_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFP0219075.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(69762..69842)
                     /gene="shoB"
                     /locus_tag="ACHK74_000189"
     CDS             complement(69762..69842)
                     /gene="shoB"
                     /locus_tag="ACHK74_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFP0219076.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(70262..70642)
                     /gene="acpS"
                     /locus_tag="ACHK74_000190"
     CDS             complement(70262..70642)
                     /gene="acpS"
                     /locus_tag="ACHK74_000190"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP0219077.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(70642..71373)
                     /gene="pdxJ"
                     /locus_tag="ACHK74_000191"
     CDS             complement(70642..71373)
                     /gene="pdxJ"
                     /locus_tag="ACHK74_000191"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFP0219078.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(71385..72113)
                     /gene="recO"
                     /locus_tag="ACHK74_000192"
     CDS             complement(71385..72113)
                     /gene="recO"
                     /locus_tag="ACHK74_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFP0219079.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(72125..73030)
                     /gene="era"
                     /locus_tag="ACHK74_000193"
     CDS             complement(72125..73030)
                     /gene="era"
                     /locus_tag="ACHK74_000193"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFP0219080.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(73027..73707)
                     /gene="rnc"
                     /locus_tag="ACHK74_000194"
     CDS             complement(73027..73707)
                     /gene="rnc"
                     /locus_tag="ACHK74_000194"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFP0219081.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(73980..74954)
                     /gene="lepB"
                     /locus_tag="ACHK74_000195"
     CDS             complement(73980..74954)
                     /gene="lepB"
                     /locus_tag="ACHK74_000195"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP0219082.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(74970..76769)
                     /gene="lepA"
                     /locus_tag="ACHK74_000196"
     CDS             complement(74970..76769)
                     /gene="lepA"
                     /locus_tag="ACHK74_000196"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFP0219083.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(76967..77446)
                     /gene="rseC"
                     /locus_tag="ACHK74_000197"
     CDS             complement(76967..77446)
                     /gene="rseC"
                     /locus_tag="ACHK74_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFP0219084.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(77443..78399)
                     /gene="rseB"
                     /locus_tag="ACHK74_000198"
     CDS             complement(77443..78399)
                     /gene="rseB"
                     /locus_tag="ACHK74_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFP0219085.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(78399..79049)
                     /gene="rseA"
                     /locus_tag="ACHK74_000199"
     CDS             complement(78399..79049)
                     /gene="rseA"
                     /locus_tag="ACHK74_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFP0219086.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(79082..79657)
                     /gene="rpoE"
                     /locus_tag="ACHK74_000200"
     CDS             complement(79082..79657)
                     /gene="rpoE"
                     /locus_tag="ACHK74_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFP0219087.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(79654..79809)
                     /gene="rseD"
                     /locus_tag="ACHK74_000201"
     CDS             complement(79654..79809)
                     /gene="rseD"
                     /locus_tag="ACHK74_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFP0219088.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            80065..81687
                     /gene="nadB"
                     /locus_tag="ACHK74_000202"
     CDS             80065..81687
                     /gene="nadB"
                     /locus_tag="ACHK74_000202"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFP0219089.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHKLRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(81672..82409)
                     /gene="trmN"
                     /locus_tag="ACHK74_000203"
     CDS             complement(81672..82409)
                     /gene="trmN"
                     /locus_tag="ACHK74_000203"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFP0219090.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSGSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            82541..83875
                     /gene="srmB"
                     /locus_tag="ACHK74_000204"
     CDS             82541..83875
                     /gene="srmB"
                     /locus_tag="ACHK74_000204"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFP0219091.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(84084..84965)
                     /gene="yfiE"
                     /locus_tag="ACHK74_000205"
     CDS             complement(84084..84965)
                     /gene="yfiE"
                     /locus_tag="ACHK74_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0219092.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRSTVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEESFVAG"
     gene            85068..85655
                     /gene="eamB"
                     /locus_tag="ACHK74_000206"
     CDS             85068..85655
                     /gene="eamB"
                     /locus_tag="ACHK74_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFP0219093.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(85711..86094)
                     /gene="grcA"
                     /locus_tag="ACHK74_000207"
     CDS             complement(85711..86094)
                     /gene="grcA"
                     /locus_tag="ACHK74_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFP0219094.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            86399..87088
                     /gene="ung"
                     /locus_tag="ACHK74_000208"
     CDS             86399..87088
                     /gene="ung"
                     /locus_tag="ACHK74_000208"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP0219095.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(87136..88173)
                     /gene="yfiF"
                     /locus_tag="ACHK74_000209"
     CDS             complement(87136..88173)
                     /gene="yfiF"
                     /locus_tag="ACHK74_000209"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP0219096.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            88380..88799
                     /gene="trxC"
                     /locus_tag="ACHK74_000210"
     CDS             88380..88799
                     /gene="trxC"
                     /locus_tag="ACHK74_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFP0219097.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            88868..89566
                     /gene="tapT"
                     /locus_tag="ACHK74_000211"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             88868..89566
                     /gene="tapT"
                     /locus_tag="ACHK74_000211"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFP0219098.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            89598..92258
                     /gene="pat"
                     /locus_tag="ACHK74_000212"
     CDS             89598..92258
                     /gene="pat"
                     /locus_tag="ACHK74_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFP0219099.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            92372..93727
                     /gene="pssA"
                     /locus_tag="ACHK74_000213"
     CDS             92372..93727
                     /gene="pssA"
                     /locus_tag="ACHK74_000213"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFP0219100.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            93773..94096
                     /gene="yfiM"
                     /locus_tag="ACHK74_000214"
     CDS             93773..94096
                     /gene="yfiM"
                     /locus_tag="ACHK74_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFP0219101.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTIW
                     QLTRH"
     gene            complement(94093..95391)
                     /gene="kgtP"
                     /locus_tag="ACHK74_000215"
     CDS             complement(94093..95391)
                     /gene="kgtP"
                     /locus_tag="ACHK74_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFP0219102.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(95717..>95821)
                     /gene="rrf"
                     /locus_tag="ACHK74_000216"
     rRNA            complement(95717..>95821)
                     /gene="rrf"
                     /locus_tag="ACHK74_000216"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        22852 a        26126 c        24090 g        22753 t
ORIGIN      
        1 ttgatgatct taaatgtctt atttttcgta atgtgtataa caaggaatag tgatgaaatt
       61 taaaaaatgt cttctgcctg tggcaatgtt agcgtcattc actctggcag gatgccagtc
      121 aaatgctgac gatcatgccg ccgatgttta tcaaaccgat caactgaata ccaaacaaga
      181 aactaaaacc gttaatatta tttccattct tcccgcaaaa gttgccgtag acaactccca
      241 aaataaacgg aacgcacaag ccttcggcgc gcttattggc gcagtcgctg gcggtgttat
      301 cggccacaac gtcggttctg gcagcaattc cggaacgacg gcaggggcag ttggcggcgg
      361 agctgtaggc gcggcagtgg gttctatggt gaatgataaa accttagtgg aaggtgtttc
      421 tttaacatat aaggaaggca ccaaagtgta tacctctacc caggtgggta aagagtgcca
      481 gtttacgaca ggtttagccg ttgttattac cacaacgtat aacgaaacgc gtattcagcc
      541 aaatactaaa tgtcctgaaa agagctaata atcaggagga gtcatgaaga aagtttttct
      601 ttgcgccatc ttggcctcct taagctatcc ggctatcgcc tcatcattgc aggatcaact
      661 ctctgctgta gcagaagctg aacagcaagg taaaaatgaa gagcaaaggc agcatgacga
      721 atgggtcgcg gagcgcaaca gggaaatcca gcaagagaag caacgtcgcg caaatgccca
      781 ggccgccgct aacaaaagag cggcaacggc agcggcaaat aagaaagctc gtcaggataa
      841 actggacgcc gaagcctctg cggacaaaaa acgcgatcaa agttatgaag atgagctacg
      901 cagcttagag attcagaaac aaaaactggc gctggcaaaa gaagaagccc gcgttaagcg
      961 agaaaacgaa tttatcgatc aggaactgaa gcacaaagct gcgcaaaccg atgtggtgca
     1021 atctgaagct gacgccaaca gaaatatgac tgaaggcggt cgcgatctga tgaaaagcgt
     1081 gggcaaagca gaagagaaca aatcggacag ctggtttaat taatcgatgt tagtaacttc
     1141 aagcctatga ttcttgaaga taaaaaaccc tctgtagtaa cagagggttt tgttcattca
     1201 tagtgcaggg attaaaatca ttcccactca atggtagctg gcggcttgcc gctgatgtca
     1261 tacaccacgc gggaaatacc gttcacttca ttgataatgc ggttggaaac gcgaccgagg
     1321 aaatcgtacg gcagatgcgc ccagtgtgcg gtcataaagt cgatggtttc gacagcacgc
     1381 agagagacaa cccagtcata cttacgacca tcgcccatta cgccaacgga acgtactggc
     1441 aggaagacgg tgaacgcctg gctgactttg tcgtacaggt ccgctttacg cagttcttca
     1501 atgaagatgg cgtcagcacg gcgcagcagg tcacagtact ctttcttcac ttcacccaga
     1561 acacgaacgc caaggcctgg tcccgggaac gggtgacggt acagcatgtc gtacggtaga
     1621 cccagctcca gaccaatctt acgcacttcg tctttgaaca gctctttcag cggttcaacc
     1681 aggcccatct tcatctcttt cggcaggccg cccacgttgt ggtgagattt gatgacgtgt
     1741 gctttaccgg ttgcagacgc cgcagattcg ataacgtcag ggtagatggt gccctgtgcc
     1801 agccacttca cgtcttccag tttcagcgct tcttcatcga atacttcaac gaaaacgcgc
     1861 ccgatgattt tacgttttgc ttccggatcg ttttcgccag ccagcgctga caggaagcga
     1921 tcttctgccg gtacgtgaac aatgttaaga ccaaagtgat cgccaaacat atccagcacc
     1981 tgctctgctt cgttgagacg cagcaggccg ttgtcgacga atacgcaagt caggttttta
     2041 ccgatagcgc ggtgcagcag cattgcggtt acggaggaat ccacaccacc agagaggccg
     2101 aggatgactt tatcgtcgcc tacctgctcg cggatgcgag ctacagcatc gtcgataatt
     2161 ttcgctggcg tccacagggc ttcacactgg cagatatcac gcacaaaacg ctccagcatg
     2221 cgcataccct ggcgggtgtg agtcacttcc gggtggaact gtacgccata gaagcgtttt
     2281 tcttcgttag ccataatggc aaacgggcag ctttcggtgc tggctacggt gacgaagtcg
     2341 gacgggatag cggtaacttt atcgccgtgg ctcatccaga catcgagcag cggtttaccg
     2401 tctgcggtca gcgcatcttc gataccgcga accagtgcgc tgtcgtttac gacttcaacc
     2461 tgcgcgtagc caaattcacg ttcgttagag gcttcaacgt gaccgcccaa ctgcattgcc
     2521 atggtctgca tgccatagca aacgccgaat accggtacgc ctgcttcaaa gacatactgc
     2581 ggcgcacgcg ggctgttttc ttcggtggtg ctttccgggc cgccggaaag aataatgccg
     2641 cttggattga agtcacgaat ttgtgcttct gtcacatccc acgcccacag ttcgcagtaa
     2701 acacccagct cacgcacgcg gcgcgcaacc agttgagtgt actgagaacc gaagtccaga
     2761 atgaggatgc gatgcttatg aatgttttcc gtcattgacg cttattccga ggcaagtgaa
     2821 acagataata taaatcgccc gacatgaagt cgggcgaaga gaatcaggag cccagacggt
     2881 agttcgggga ctctttagta atggtcacgt cgtgaacgtg gctttcctga atgcccgcac
     2941 cgctgatacg tacaaactcc gctttagtac gcagttcgtc gatagtacca cagccggtca
     3001 gacccataca ggagcgcagg ccgcccatct gctggtgaat gatctctttc aggcgacctt
     3061 tataagctac gcgaccttcg ataccttccg gcaccagttt gtcggcagcg ttatcgctct
     3121 ggaaataacg gtcagaggaa cctttggaca tcgcgcccag ggaacccata ccacggtaag
     3181 atttgtaaga acggccctgg tagagttcga tttcacccgg agattcttca gtacccgcca
     3241 gcatggaacc taccatcacc gcgcttgcgc cagcggcgat agctttggcg atgtcgccgg
     3301 agaagcgaat accaccatct gcgataaccg gaataccggt gccttccagg gcttcaactg
     3361 cgtcagcaac ggcagtgatc tgcggaacac ctacgccagt aacgatacga gtcgtacaga
     3421 tagaaccagg gccgataccg actttaaccg cgctgcaacc agcttctgcc aaagcgcgtg
     3481 cacctgcagc tgttgccacg ttgccgccga tgatttgcag atccggatat ttagcacggg
     3541 tttcacggat acgctgcaaa acgccttctg agtgaccgtg ggaggagtcg atcagcagaa
     3601 cgtcaacgcc agcggcaacc agcgcgtcaa cacgctcttc gttacccgca cctgcgccaa
     3661 ctgcagcacc aacacgcaga cggccttgct cgtctttaca ggcgttcggt ttacgttccg
     3721 ctttctggaa gtctttcacg gtgatcatgc cgatcaggtg gaattcgtca tcaaccacca
     3781 gcgctttttc aacgcgtttt tcgtgcattt ttgccagcac cacttcacgg gcttcacctt
     3841 cacgcacggt gaccagacgc tctttcggcg tcatgtaaac gctaaccggc tggttcaggt
     3901 cggtaacaaa acgcacgtca cgaccggtga taatacccac cagttcgttt tcttcggtaa
     3961 cgaccggata gcccgcaaaa ccgttacgct cagtcagttc tttcacttcg cgcagcgtcg
     4021 tggttggcag aacagtctgc ggatcagtca ccacaccaga ttcgtgtttt ttcacacggc
     4081 gaacttcttc tgcctggcgt tcaatggaca tgtttttgtg gataaagccg ataccgcctt
     4141 cctgagccag agcaatagcc aggcgcgctt ccgttacggt atccattgct gcggaaagca
     4201 tagggatatt cagacgaata gttttcgtca gctgggtgct gaggtcagca gtattcggca
     4261 gaacggtaga atgagcagga acgaggagaa cgtcgtcaaa cgtcagagct tctttagcga
     4321 tacgtagcat gggcaatatc tcgaccagag tggttaataa atattgccgc ggcattatac
     4381 agagcgtaac cgattgcatc tacccctttt tgcaaaaaat gcttgctatc cccgaagggc
     4441 gggttactat cgactgaata acctgctgat ttagaatttg atctcgctca catgttacct
     4501 tctcaatccc ctgcaatttt taccgttagt cgcctgaatc aaacggttcg tctgctgctt
     4561 gagcatgaga tgggacaggt ttggatcagc ggcgaaatct ctaatttcac acaaccggct
     4621 tccggtcact ggtactttac actcaaagac gacaccgccc aggtacgctg cgcgatgttc
     4681 cgcaacagca accgccgggt gaccttccgt ccacagcatg gacaacaagt tttagttcgc
     4741 gccaatatta cgctctacga gccgcgcggt gactaccaga taatcgttga gagtatgcag
     4801 ccggccggtg aagggctgct gcaacagaag tacgaacagc tcaaagcgaa gttgcaggct
     4861 gaaggtttgt tcgatcagca atacaaaaaa ccacttccct cccctgcgca ttgcgttggt
     4921 gtgatcacct caaaaaccgg tgctgcgcta catgatattt tgcatgtgtt aaaacgtcgc
     4981 gatccttctc taccggtgat catctacccc accgccgttc agggcgatga cgcaccgggg
     5041 caaattgttc gcgccattga actggcgaat cagcgcaatg agtgcgacgt gttgatcgtt
     5101 gggcgcggcg gcggttcgct ggaagattta tggagtttta acgacgaacg cgtagcgcgg
     5161 gcgatttttg ccagccgcat tccggtcgtc agcgccgtcg ggcatgagac ggatgtgacc
     5221 attgccgatt ttgttgccga tctgcgtgcg ccaacgccgt ctgccgccgc tgaagtagtg
     5281 agccgtaatc agcaagagtt actgcgccag gtgcaatcga cccgtcaacg gctggagatg
     5341 gcgatggatt attatctcgc caaccgcacg cgtcgcttta cgcagatcca tcaccgatta
     5401 cagcaacagc atccgcagct ccggctggca cgccagcaaa ccatgcttga gcgcctgcaa
     5461 aagcggatga gctttgcgct ggaaaatcaa cttaagcgta ccgggcaaca gcagcagcgg
     5521 ttaacacagc ggctgaatca gcaaaatcca cagccgaaga ttcatcgcgc gcaaacgcgc
     5581 attcagcaac tggaatatcg tttagcagaa accctgcgcg tacagcttag cgccacgcgt
     5641 gaacgtttcg gtaatgcagt aacgcacctc gaagccgtaa gcccactgtc aacgctggcg
     5701 cgtggataca gcgttactac tgctactgac ggcaatgtac tgaaaaaagt gaagcaagtt
     5761 aaagcgggtg aaatgctaac cacacgtctg gaagacggct ggatagaaag tgaagtaaaa
     5821 aacatccagc cggtaaaaaa atcgcgtaaa aaggtgcatt aagccagcac gaattcaacg
     5881 cgttttttcg atatcaagcc atgcccgtgc tgacagaagt aatctactgc accgcaggct
     5941 tttaacacct gcaacggttg atggcagtct ggacaaagag ctttcatttc aataatttcc
     6001 ccgcaggatg ggcaacgcgc atggtcgtta tcctgatcaa gcacatgctg acattgtgga
     6061 caatgcagtt ccatatgaat tcctccggta gccatccctc tacattcata gagggaatgg
     6121 cagataaaat acttacggat aattatttat ttttcttgat gtgcttcatc agacgcttac
     6181 gtttacgcat ctgggttggc gtcagggtgt tacgcttgtt cgcatacggg ttttcccctt
     6241 ctttgaactg aatacgaatc ggcgatccca ttacgtccag cgatttgcgg aagtagttca
     6301 tcaagtagcg cttgtaggaa tcaggcaggt ctttcacctg attaccgtga atcaccacaa
     6361 tcggcgggtt ataaccacca gcgtgggcat atttcagctt cacacgacga ccgcgtacca
     6421 gcggcggttg gtgatcttca acagccatcg tcatgatgcg cgtcagcata gaggtcccca
     6481 cacgacgggt ggagctgtca tacgcttcac gtactgattc aaacaagtta ccaacaccac
     6541 tgccgtgcaa ggcagagata aagtgcacac gagcaaaatc gataaagccc agacggaagt
     6601 ccagcgtttc tttcacctgc tctttcactt cctggctcag gccatcccac ttattcacca
     6661 caatgacaag tgagcgccca ctattgagaa taaagcccag cagcgagaga tcctgatcgg
     6721 aaataccttc gcgcgcatca atcaccaaca tcaccacgtt ggcatcttca atggcctgca
     6781 acgttttgat tacggagaat ttctctacag catcggtgat tttgccgcgt ttacgtacgc
     6841 cagcggtgtc aatgagcaca tactcacgtc catcgcgttc cattgggata tagatgctgt
     6901 cacgcgtcgt accaggcatg tcataaacaa caacgcgctc ttcaccaaga atacggttag
     6961 tgagtgtaga cttacctacg ttcggacgac ccacaatcgc cagtttgatc ggcagacttt
     7021 gcgggtcaaa gtcgtcttcc tcttcttctt cgccgttctc ttccgcttca aattgcgccc
     7081 agtattcagc gtcttcgtcg acttcctctt gcggtgcgag atcttccatc cacggcagca
     7141 gcacatgctc cagcagactt aatacgccac gaccgtgaga cgcggcgatc gggtagattt
     7201 cacctaagcc aagcgagtag aaatcaacca ctgcctgatc gggatccaga ccgtcagttt
     7261 tgtttgccac caggaaggtc ggtttttcac gggagcgcag atgtttggca atcgcttcat
     7321 ctgccggcat caggcccgcg cgcgcatcca ccataaacag tacgacgtcc gcttcttcaa
     7381 tcgccagcag cgactgttcc gccatgcggg tttctacacc gtcttctgtg ccatcaatcc
     7441 cgccggtatc aatacagata aactcacggc cttcaatttc cgcacgaccg tacttacggt
     7501 cacgagtcag acccgggaaa tccgcaacca gcgcatctcg ggtgcgagtt agacggttaa
     7561 ataacgtgga ttttcctacg ttagggcgcc cgacaagcgc gaccacaggt accatgttta
     7621 aagcctcatt tttataaatc atcagacaac gcacgctata ttcgcgtcgt tgttaaaaac
     7681 aggaaaacgg cccctgtcca ggagccgttt tcaaagtgaa cgacagagac gattaacgtg
     7741 taatagagta cacggttccg tcttttgcct ggatcagcag tttgccgtca gcggcaaccg
     7801 gttcagtctg gaaaccggaa ctatcaactt tttgctgggc aacgaaacga ccatcttcga
     7861 cgttaatcca gtgcagataa ccttcactgt caccgaccac caggttgcca ttatacagca
     7921 ccggagaagt cagcaggcga tgcagcagat cgctttgtgt ccacagcgta acgccgccat
     7981 caatggtcaa cgccatcacc ctgtcatttt gatcgaccag atagatgcga ttgccgtcga
     8041 cgatgaaatc attcaccgaa cccagttcgc gtttccacat aatctgacca ctgcgcagat
     8101 caagcgccgt caggttacca ttataggcca gcgcgaaaac aacgccgtta acaacgacgg
     8161 gagtcgtgtc aacatcgctc agacggtcaa tttcggtaga accggtcgcc tgggaaatac
     8221 gctgctgcca aatcatctgg ccctgttcca tcagcactgc gctgacgcga ccattatcgc
     8281 cacccacgac ggccgcacca aaagccgttg ccggcgcaga ctcgccacgc aaagagagcg
     8341 aaggcatatc gaggttaact gtccatttga cagcgccgtc agcctcgttc agcgcttgta
     8401 actgaccgtt actggtgtgg attaacacca gaccgtcgct gaccaccggg cgcgaaagtg
     8461 cttcacccgc gactttagtt tgccatgcca cagtaccatc gctggtattc agcgcgtaaa
     8521 cctgcgcctt ttcgctgcca atgtagacat gcccaccaga cacggtcaga ccgccagaaa
     8581 gtaatgcagg ctctttagag aaccagccat ctttctcggc caggctgaca gaccagattt
     8641 ctttgccatc atccgcattc agcgctttta ccaaaccagc gcggtccgct gcatagacaa
     8701 cgttgtccgc cagtgccgga tgaagattgg aatagaagtt gccaatgccg ctaccaacgg
     8761 aagtgctcca cgccgtggtc ggcgtaaact ggttttcaac ggttggcaat ggggacatct
     8821 ttaccacatc ttcttcgctg ttaaacagcg aacagccgct taaaagggta acggaaagca
     8881 gtcctggcag cagtaattta cgcaattgca tcgggtccct ctcagatgga caaattatta
     8941 attttcatct gcatcatttc gctcagtgcc ggagtaacat cgcttttcac gcctgcttcc
     9001 catgcactac gcgcaccttg cttatcacct ttgctcagca atgcttcacc acgcaggtcg
     9061 gcaacaatgg cagcccaccc ttcacctttg atggtatcaa gggttttcag cgcggcatca
     9121 gcctgcttga gctgtacctg aacgcgagca agacgcagat ttatcacggc tttgagattt
     9181 tcatcgctcg tgtctgccag cccctgttgt aactgggcgg cagctttctc tagttcattt
     9241 ttgtcaacaa attgctgcgc aagttccaaa gaagccagcg caccataagt atttttattt
     9301 tcagcagcaa atttttccgc cgccgggatg ctatccggtt tgccttcgct cactgcggta
     9361 accgcatttt gataggcaag agaagcggag cgtgcagaat caacctgatg gctgttccag
     9421 tagcgccagc caatcagtgc gccaacgccc aaaatcaccc caacagccag tgctttgcca
     9481 ttttcagcaa aaaagcgttt aaccgcttct acctggtcgt tttcgttctc gtaaatttcc
     9541 acgctgtcct tctccttcct taacccagta acgtgcgcaa atgcgcggct acgctatcct
     9601 gcgcaactgc cgtttgctca ccagagcgca aatccttcac tactgctgtg ccgttagcca
     9661 cttcagactc acccagcacc acagcaacgc gggcacccca tttatcagca cgggcaaact
     9721 gtttcttaaa gttgccgccg ccgtggttgg tcatcaattt cacgcccggt aattcatcac
     9781 gcagacgctc agctaatgcc atagccgcag attgtgtatc agcacctgaa gccaccaggt
     9841 atatatcgac aacaggatcg gctttaaatt ccggattaac ggcctgtact aacaatacaa
     9901 gacgttcgag gcccatcgca aaaccgacag ccggtgttgc acgaccgccc agttgttcca
     9961 caagaccgtc ataacgaccg cctgcacaca cggtgccctg ggagccgaga ctgttagtca
    10021 cccactcgaa aacggtacgg ttatagtaat ccagaccacg caccagacgc tggtttacgg
    10081 tgtaagcgat ccccgcgctc tcaagcagtt tgcacagacc ggcaaaatgc tcacgagatt
    10141 cctcgtccag ataatcacct aatgccggag cgtcgttgag aagcgcctgc acttccggat
    10201 tttttgaatc cagcacgcgc agcgggttag tgtacatgcg gcgtttgcag tcttcgtcca
    10261 gcttttcttt atgctgctca aggaatgcca ccagcgcatc gcggtaattg gcgcgtgctt
    10321 ccagcgaacc gatagaattc agctcaagag ttacgtgttc ggaaataccc agcgcgcgcc
    10381 accagcgggc ggtgagcata atcagttcag cgtcgatatc cggaccttgc agaccgaaaa
    10441 cttcgcagcc caactgatgg aactgacgat aacgcccttt ctgcggacgc tcgtgacgga
    10501 acatcggccc gatataccac agacgctgtt cctgattgta cagaagacca tgctcgatgc
    10561 cggcgcgtac acagcccgcc gtcccttcag ggcgcagagt caggctgtcg ccattgcgat
    10621 cctcaaaggt gtacatctct ttttcaacca cgtcggtgac ttcaccaatc gcacgtttga
    10681 atagcggggt ctgctctaca atcggcaagc ggatttcact gtaaccgtag ctgccgagca
    10741 cgtttttcag tgtgccttca atgcgctgcc agatggccgt ttcgccaggc aggtaatcgt
    10801 tcatgccgcg aatggcttga atgttttttg ccacgtttat tctctttctg aatataaaaa
    10861 tgaaccctca acgcttccct caatgtttcg ggagctatgc gggttcaatc atacacggga
    10921 agcgaggcgc ttcccatcac gttattattt ttcaacctgc tgaacgtcaa ttcgacgcgc
    10981 ttcgtccagc tgactggctt tcgcacgaat gcgtgcttcc agctggtcga tcatatcgtt
    11041 gttgtccaga cggtctttgc gcacgccatc ttcatagagg ccgcttttct tgttgccgcc
    11101 ggtgacgccg agtgtagaaa ccagcgcctc acctgggcca ttcaccacgc agccgataat
    11161 cgaaacgtcc atcggagtga tgatatcttc caggcgttgc tccagcgcgt taaccgtacc
    11221 gataacatca aattcctgac gcgaacaggt cgggcaggcg atgaagttga tacctcgcga
    11281 acggatacgc agcgatttca aaatatcgaa accgactttg atctcttcga ccggatcggc
    11341 cgccagtgat acgcgcagcg tgtcgccgat gccttcagac agcagcagac ctaaaccaat
    11401 ggcggatttt actgccccgc tgcgtgcacc accggcttcg gtgatcccca gatgcagcgg
    11461 ctgatcgatc tgttttgcca gcaaacgata agactcaaca gcgaggaaga cgtcagacgc
    11521 tttcacgctg actttgaact gatcgaagtt caggcgatcg agatgatcaa catggcgcat
    11581 ggcggattcc agcaacgcct gcggcgtcgg ttcgccatac ttttcttgca gatctttttc
    11641 cagcgatccg gcgttaacgc caatacggat cggaatgttt ttatcgcgcg cacagtcaac
    11701 caccatgcga atacgctctt cattaccgat attgccaggg ttaatacgca gacaatcgac
    11761 gccgtattcc gctactttca gtgcaatgcg atagtcgaag tggatgtcag ccaccagcgg
    11821 cacgttaacc tgctgtttga tgagtttgaa cgcttctgcc gcgtccatcg tcggtacgga
    11881 gacacggacg atatcagcgc caacgcgttc cagcgccttg atttgattga ccgttgcttc
    11941 gacgtctgtc gtacgcgtat tggtcatgga ctgtacggcg atgggagcac catcgccaat
    12001 cggcacattc ccaacgtaaa tacgtgttga ttttctacgt tgaattggag cctggttatg
    12061 catgaaaaat ctcccgcgtt acccgtctgt tactgcgccg gtgattgttc ggcattgagg
    12121 gtcagacgcg caacctggtt agttctgata aaacgactca gatcgacagg tttcccttga
    12181 tactggatct gtactgcggc tggcgcacca attttcagtt tgtatggtgc ctggccggtt
    12241 aagttcaaat taccgtcttt acgctgcata ccgctaaaca attttttacc ggtagcatca
    12301 gtaacctcca gccagcaatc ggcagtaaag ttcatcacca gcgcattcgg atcagccgcc
    12361 ggcgtagtca cgcctgcctg atcggttggc aacggcgcag cgccatctgg cgttgttgtt
    12421 gccgttggtg ccggggtcgc ggcggtatca acatttgctt gcgaaggcga aacaaccgca
    12481 ttctgttgcg gatcaacagc tggtgctggc gcagttacgg caggtgtttg cgtgttggtt
    12541 gcggtagtat ccacagacgc tggcggcgtg gacgttgtag ccgggtctgt agttgtcgac
    12601 gtatttaacg gaacactctg cccctgctcg ctattactgc tcagttccgc cgaagattga
    12661 tcggccatag tggtgatctc ttcctgctga gctttgtggt cttgccacca ccaggcaccg
    12721 ctcaggccga taaccacaaa caacaccagc caggtgaagg tcatcagcca gccgtcacgt
    12781 tttttgcggc gtttaccgag ggaaaaactc tgcataggcg caacttttgc agcccgtagt
    12841 ggagcctgct tttccagccc tggcagcagt tcttcttctg gaatatgtac cagacgcgca
    12901 taagagcgga tatatccgcg caggaatgtt gaagcaagat cggcgggtgc cttatcttct
    12961 tcaatgtcgc gtaccgtgga aaccttcagg caaagtcgct cggcaacggc ctgctgacta
    13021 agtcctagtt gttcgcgagc attacgcagg cgagcgccgg tagtaagtgc ttcattttgg
    13081 tcgtgcgtgg cttcagtatt cattcgctgc aggtacgttt aaatgagaat tgagatgccg
    13141 gtgaaccatc atgctcaccc acaccgcgaa acatccgtta agttaaccgt tatcatacag
    13201 tataagaccg tctgtgtgct cgtgacaaag cccacattca ctaaggctaa cttactgttg
    13261 catcgttaca tactgcctta aagtcagcaa aaacgcaccg ttaatattga ccagacaatt
    13321 gcaacttaat acttcattca taaaagatta cgccacggta cataaagtaa ccgtggcgta
    13381 atggctatca gaccgcttta atgtcgatgg cttcaccctg catccgttta cgcagggtac
    13441 gtttcgtacg gtcgataaca tcgcccgcca actgaccaca ggcagcatcg atatcatcac
    13501 cacgggtttt acgcacaata gtggtgaaac cgtagctcat cagcactttt gagaaacggt
    13561 cgatacggct gttcgagctg cgtccatacg gcgcgcccgg gaaggggttc cacgggatca
    13621 ggttgatctt acacggcgta tctttcagca gttccgccag ttggtgcgcg tgttcagtgc
    13681 cgtcgttaac gtggtcaagc atcacatact caatagtgac acggccctga ttggcgttgg
    13741 atttctccag ataacggcgc accgcagcaa ggaacgtttc gatattgtac tttttgttga
    13801 tcggcacaat ttcgtcacga atttcgtcgt tcggcgcgtg cagggaaatt gccagtgcaa
    13861 cgtcgatcat atcgcccagt ttatccagcg ccggaactac accggaagta gaaagcgtga
    13921 cgcgacgttt agacaggcca aaaccgaaat catcaagcat gatttccatc gccggaacga
    13981 cgttgttcag gttgagcagc ggctcgccca tgcccatcat cactacgtta gtgattgggc
    14041 gctgaccggt gacttttgct gcgccgacga ttttcgccgc acgccacacc tggccaataa
    14101 tttccgatac ccgcaggttg cggttaaagc cctgctgggc ggtggaacag aatttacact
    14161 ccagcgcaca ccccacctgc gaagagacgc agagcgtggc acggtcgtct tccgggatat
    14221 acaccgtttc gacgcgctga tcgccaacgg cgatcgccca tttaatggtg ccgtcggatg
    14281 aacgctgttc ttcaaccact tccggtgcgc ggatctccgc cacctctttc agtttgccgc
    14341 gcaacacttt gttgatgtcg gtcatctcat caaagttgtc gcagcaatag tgatacatcc
    14401 acttcatcac ctgatcggcg cggaagggtt tttcacctaa atctttaaaa aactcccgca
    14461 tctgctgacg gttgagatcc agcaggttga tttttccatc tttcgtggtg acgttttcag
    14521 gtgtgactaa ttgttcagac atatgctatt ccggcctcgt tattacacgt tatggcccct
    14581 ggagggttga aaaaagaaac gccccggtga gcggctgctc gtccgggggc gctgcattgt
    14641 acaaattctg gcgcacggat gccacgtttg cacgcggcat ttacgaaatt attaacgggt
    14701 gcgcgggcac acttcgcctt cgccaaagaa ataagcgatt tcgcgagcgg cagattcgac
    14761 ggaatcagaa ccgtgggtac cgttttcggt caggctgtca gcgtaatcag cgcgcagagt
    14821 accagccagt gcgtttgccg gattggtcgc gcccagcaga tcgcggtgac gctgaacggc
    14881 gttttcacct tccagcacgg aaaccacgat cgggccagag gtcatgaatt caaccagacc
    14941 atcaaagaac ggttttccat cgtgttcagc ataaaagcca cgtgcctgtt caacggtcag
    15001 gtgcagcatt ttggtgccga caattttgaa ccctgcagct tcaaagcgcg caaagatatt
    15061 accaatgacg ttttttgcta ccgcgttcgg tttgatgatg gaaaaagtac gttcaatagc
    15121 catttttacc tctgtaaatt gttctgttgt tgtctgtacc agcgtacgag atggcgcgga
    15181 ttataatgag caacagggcc gttgactatt gatgaaggta acattttttt aaaataaaat
    15241 gagttttagc aacaatcagg ggcaaaacag actattgcat gacaaatttc actgtcgcga
    15301 tttgccccac atcatccatc accagcaatt gataatcacc tttatccgtc aaatgcaggg
    15361 tcacgttgcg cccgcgttca gttaacggtt cgccattcag aaaccaccag cgttcacctg
    15421 cccctccact tgattgcaac ggcaaagtcg cttccgctgc gcccggtaaa cgtttaataa
    15481 tcgcgccatc gcgaacgcct gtcagctgca gagggagctt ggcatcgtga ccgtatggcg
    15541 ggcaaattgt cgaggccggt ggtaagcgca cagcgcgacg ttctgatgcg ggcagccagg
    15601 gttccagcgg tagcggccag acatttatca tttcttgtcg cgcttgcggg caatcagcgg
    15661 caacacgttt gccattttca tccagccaga tggggaaacg aatgccatta atgccttcct
    15721 gctccggcag taatagagtt ggcggctgac tcccgtccag cagccaggtt gccaggcggc
    15781 ggcgacagtt accgtcacct tccggcaaag actgtccgcc cggccagcag ataacgccac
    15841 gagtgactga gttcgggcgc gggtcttccg gcagattcgc actgcgcgac agtaagatat
    15901 tattgacctg attcaacaat ggtacggcac tggcaaagcc aaactgacca acaacgggcg
    15961 tgccgtccgg tctgccagtc cagatcccaa tgacatagcg agcgttaacc ccaatcgccc
    16021 aggcgtcacg atagccatag ctggtgcccg ttttccatgc cagtggggcg acgcgcggca
    16081 aggcactatc cggcaagggt ttcgcttcat cagccataat ccggcgaatg atccacgccg
    16141 cccccgacga cattaaaggc cgttcaagca gcggatcgtc aggctgtaag cgcaatttgc
    16201 ctgccttgcc gtggcgagca aacgcggtat acgctgccgc catatcttcc agttttgcac
    16261 cagcgccgcc gagaatgagt gaaagattcg gcgcagcacc gttgggcaaa tataacggca
    16321 atccaacatt gcgtaacttt gccgcaaacc gtttcggtcc ataggcttcc agcacctgca
    16381 cagcaggtaa gttcagcgag cgcaccagcg cctcgctcat gctgatcggg ccatgaaaac
    16441 cgctatcaaa gttacctggt cgataatcac cggtgcgccg ggggacgtct tgcagcagtg
    16501 atgccgggtg gatcaagcct tcatccagcg ccagaccata aacaaacggt ttgagcaccg
    16561 atcctggcga tcggatcgca ttgaccatat caacatgacc aaagcgtgaa tcatcgttga
    16621 gatcaaccga tcccacccag ccgcgaacac gcatatcggt atgatcaacc acgatcatcg
    16681 ccagtgagct gcgcggtggc aaccgccctt tccagttttg cgccagttct tccagacgtc
    16741 gttgaagacc ggcatccagc gtagtaacga ttttgtcgct tttgctttta ccgagcatca
    16801 tgcgcgaaaa cagcggtgcc agttgcggca tttgtcgggg tgccagccag atgggttctt
    16861 cccttgactc ttttacctgc tcacgggacc acacaccttg cacggccatc cgttcgagca
    16921 ctttattacg cgcggcttcg gcacgctccg gccaacgatc cgggcgaaga cggctgggcg
    16981 cttgcggcaa aaccgccagc attgccgcct cggaatagct taaattcgca ggcgactttc
    17041 cgagataagc ccaacttgcc gcaccgatcc cctgcaacgt accgccaaac ggagcgcggt
    17101 taagatacaa ggtcagaatt tcacgcttag acagatgcca ttccagttgc aacgcgcgcc
    17161 agagctggcg aattttgccg ccaaatgttt tggggtgagg atcaagcaga cgagcaacct
    17221 gcatagtgag cgtgctgcca ccggaaataa cccgtcccga agtgagatct tgccatgctg
    17281 cgcgcgccac cgagaatgga ttcacccccg gatgcttcca gaaccagcga tcttcataat
    17341 tgatcagcgc ttcaaggtaa cgtggagaaa catcttcgat tgttaccgga taacgccaga
    17401 tgccgtcagc atcggcgaag cgccatagcg gcgtaccatc ctgcgccacg accactcgtg
    17461 cgggattgac ttcatgcagc ggtagaggcc agagtttatc tgctccccac gctgcaagaa
    17521 aaaggagaaa tggcgcagct gccagcgtta tccagcagcc gcgtttggtt aacaagcgag
    17581 gcatttacgg tcggacaatc agcagatctt cagccgcgcc ggtcgcccgc cattggggaa
    17641 catacattga ttccaccatc ggttgcggta cctgatacgt tcccggcgtc accgcccgcg
    17701 ccagatacac cagcgttacc ggttggtatt catcaacggc aaccgccgcc acaaagcgat
    17761 cgtcacggaa ctcaatgtgc ttaatactcg cctgctgcat ctggttcagt aagttttgca
    17821 cttcgccacc actttgctcc aggctggcgc taccgttcgc cagattctga ttttccagtt
    17881 ccagacccgc aggcagcaga tccacgacta acgcatccgg cacgctgtta ctggctttta
    17941 cctgcaacca gaccagcacc agatcgccgc tacgtaacga gtccagcgat ttgctcttac
    18001 catcagtacc aagaatatga cgctcgattt gcagcacatt gttcgccggt aaaggtgcgg
    18061 attgcggata accgctggca tccacacgca gccataacgg ctgatcgcca ctgtttctca
    18121 cctgcaaggt ggcaagttga tcgctattca gattgctgtt tagcgttttc tcgcctgtca
    18181 gcggctcagc tgagaaagag gtttgcgcct gccatttacc cggtaaatcc tgaatcgtac
    18241 gggcagccag gaacaacgcg ttactttcct gcgtcgatag ccagcgttca ccaaatgcct
    18301 gctgcgaaag cgtgttcagc aaagtgttct gctcatcggg tagcagttta ttttcttcca
    18361 gcaagtagag cattaacgcg ttgtcgcgca gtggactacc gtaatcaccc agccatatcc
    18421 gctcatcact atttctcggc gttttcagcg ccagcacaat cgcttcttca ccacgcgttg
    18481 catcacccat ggttttcagc gcaacgccaa gttgcagcag cggtaaacca gaagcggcat
    18541 ctgcacgatg ctcccagatt tcacgcagcg cacccagtgg tgctttttgc tgacgggcca
    18601 gcaccagcgc agcgtaagac tgaacggcga atttactggc tttgagatta tccgcgtacg
    18661 ggatcgacat catgcccgga tcttgtaaat agcgcagcag acgctcatta ccccggttaa
    18721 tggcgtctgt cggcacgctg taaccctgtt cgcctgcgcg gaccaggaaa tccatcacgt
    18781 aagccgtcag ccagtactct tcgtcaccgt ttttatccca cagcgcaaag ccgccgttat
    18841 cacgttgcat ttgcagcaaa cgggaaatgc cgatatcgac cgatgcacgg cgtttctcat
    18901 cactgtcgcc tttgatgccc aacgcctgca gttgggctgc gttggtataa agtgacggga
    18961 acaggccgct ggcggtttgc tcaagacagc cgtacggata cgcttttaac tctttgatat
    19021 aacgtgcaat gttcagcggt ggtttgccgc tcaacaacaa ttgcccttcc agcgtaacag
    19081 gcgagaagtt ttgcaatcca tccgccggaa tcgcccatgt ctcaccaggc tgtaacgccg
    19141 taccgtaatt aaccgtttgt gccgggaacg ccggacggac gccgattttc cactgcttat
    19201 gctgatcggc aacggtttca cccggtaacg ctaacccgct aatggtggcc tgaatttctc
    19261 catcgccata acccggcaat gctcgcaccg ggataaacag cgtagtacgc acacctggcg
    19321 ctaattcaac ggctgcgggt gaatcgctga ccagttcaag caaaccactg gcggtcaggg
    19381 caacgttcag tttttgcggt ttatcggtaa gattagtgat atccagcgtc agacgcgagg
    19441 tatcgccact cgccataaag cgcggcatgt tcagttcagc aatcaccggt gcggcaacta
    19501 tcactttgct ttcgttgcta ccgaaatcat ctgccgtcca ggcttgcgcc atgacgcgca
    19561 attcaccgtt aaaatcgcca atcggcagtg taaccgagcc ttcgccctgt tcgttgagcg
    19621 ttaccggcag cgcctgctgc gcgacaatat tgacgtgatt gaccggcggt ttaccaccac
    19681 gtttcagctc atcaccatcg ccaccgaaac gcagagctgc cagacgcccc tgaccttcaa
    19741 taacctgacc gtaaatatcg taaatgtctg cgccatagcg tttctgacca aagaacgcct
    19801 gccacggatc tggcgtgacg tagtcagtaa tattcagcac accactatca acggcagaca
    19861 ccagcacatt cacctgttta ggcttctcgc catttttagt gctggcttta attttcacgg
    19921 ttaatggttg attgggacgc atttttgctg gtgtttccag cgccagatcg aggcgacggt
    19981 tttcatcgcc aagcggcaga tgcaacacac caaccgcgcg ttttggcgtc gcggagcgag
    20041 atttatcgcc aggacgtacc accagcgtac ttaaatacag atcatgacga ttccaggttt
    20101 tatcgaccgg aatcgtcaga tccagccctt gagccggaac atcaatctct tgccaccaca
    20161 gcggcccttc actggactcg accatcgcat aacctttacc cgccgttggc gcggcgatat
    20221 gcaacttaat ggtgtcgcca gggcgataac tggctttatc cagtttcagc gtgacacggt
    20281 cgggtcgcac tgcgccgcta ccgtcgctgt tgtcctgcca gctatagcca gcccagaaac
    20341 gaacactact gaccgcttca ttcggcgctt tgacttccag acgataagcg ccccactcta
    20401 ccggaaaact gaccttgccg gtttcgtccg ctttcagatc cagagtttgt tcattttcga
    20461 gcagatcttt ttgatcaaac tgtgactgcc agccttcatc ttctgaccag ttccagtagt
    20521 aatcgcggcg ttcgcgaatc aggcgcacct gcaagcccga cacggctttt ttcacgcctt
    20581 gcgcatcgct ataaacgatg tcaaaagcgg cgttactgcc ttcatcaaca atgggctgtt
    20641 tcaccgtgct gtcagtacga taatcgtaaa ccgatttcga ggcgaactgc ggacggatcc
    20701 ccggcaatgc atcggcaggc cagatagcct gctcagcgcg gcgcgtcacc gggcgaccgc
    20761 ccgattccag caaactaccc tggaaaataa cctgtaatgg ggaatgcgtt tccttccact
    20821 ggctttctgt agaaacttcg ccgcgccctt tatcatccag cgtcaactga acttcatcca
    20881 gcgtgcggga aagattttcg gcagctatat cgccgaattc aaaaccaggt aaggctgaca
    20941 cagcttcacg cagtgggcgc aggaaaagtt gcccttgcaa agtattacca ttagcaggtg
    21001 caccatacag gtagtacccc accacggaga atttcacttc atctttcggc gttagcgggg
    21061 ttttctcacc ggtcagattc agcgccatgc gctctggcat aaaatcttcg acgtggaaat
    21121 cccacatccg atactggtta tcgcccgtgt tagcgcgaat atgccacata ccggttgccg
    21181 cattgctatc gagtggccag gtaaagtggt agaggccatt ctccggctga ctaacgacgc
    21241 tcctgagtac ctgcccatcg ggtttaatca cgtctaactt gatgggttga ttgggcaacg
    21301 ctttaccgtc tgcatcacgc agcaaaccat tgaggattac cgtttcacct gggcgataaa
    21361 gatcgcgtgg gccaaacatg aaaaactgtt tgctatagcc tggcgcgcca gcaatgttaa
    21421 attctgctaa gtccagcgcc ggaagtttta aatcgagtag cgttgtctga ccgtctttac
    21481 gcgccaacag taatgccgcg tttttatcat tttccagctg cacatgcccc tgagcgtcac
    21541 tggttgcctg agtcagagtc tgccctttct catttaataa agagacttca attccttgct
    21601 gggccgcgcc gttttccaga ctttgggtaa agatatccag acgattgtga taacggtgag
    21661 ctgaaacgcc gatatcactt aacgtaaaca gcgtcgcggg attactgtaa tcgtaacgtc
    21721 cagcctgatt catcacagcc agatacacgc ccgcctgctg aagcggtttg atatcgccca
    21781 gcggcagcaa taatttttca cgggtgttac gcgcaggatt gagatcaaac cgtccggtgt
    21841 agaccagatc cgccatctgc agcagtttgt ctgactgcca gttcgccagc gaattgcggt
    21901 attcccattg gctaatgaat gctggcagag attctggctt aacgcggaag aagttaacat
    21961 cgacattatt aacgttgagc gccattaccg gcagcccttc aacgactttg ccaggcagca
    22021 gcgaaccacg gctggcaaaa ccgacgctgg gttggatgtc gcgggtagtt atagtttttt
    22081 cgtaatcttt actgaaggtt gcgttgttga gtgctttgac ctccttgcca atagtaacga
    22141 tcaaatcacg tttcggttcg aggtggcgta aacgtaactc tttaagatta tctgacagct
    22201 cccaggcacc atccactttg ccgctttttt tatcgacgac atgaataacg cgtgagaaat
    22261 cctgatccgg gtcgagaggg atggagaacg tcagcaccag cgtggctgca ccatcaagtt
    22321 ggacttcaga gagatccagc aatgtcagca ccttaccggc actctgttgg gccagttttt
    22381 gtctctccgg cacggagagt tttgctgaac tcgcgttttc tgaagaggcc gctttagtga
    22441 cttcagaagg cgcatctttt ttcaccgctg ttggcgcgtt atcgttgttg tcgcaccctg
    22501 ccagcgccag cattagcatg caggcggcta cgcgtaactt tttcattttt catccctgcc
    22561 acaatggccc gttagcaacg tcgaataatt attatgcgtg agaatttacg ttatgtaatt
    22621 cagtatcacg cccatacaat ccgaatttta gtgagaattg tggctcagac agccaaatca
    22681 gcctctttcg cttgtcgctt ttcactaaac atccgacaat ttagcctaac cccggcaaaa
    22741 atggagatgc ctatgtccac gacatggttt gtaggagccg actggctcgc cgaacatatt
    22801 gatgacccgg aaattcagat tattgatgcc cgcatggcgt cgcctggaca ggaagatcgt
    22861 aacgttgctc aggagtatct gaatggacat attcccggcg cagtgttttt tgatatcgaa
    22921 gcgctttctg atcacacttc cccgcttccg cacatgctgc cgcgcccgga aacgttcgcc
    22981 gtggcgatgc gtgaattagg cgttaaccag gataagcacc tgattgtcta tgacgaaggt
    23041 aatcttttct cagccccacg agcatggtgg atgctgcgca cctttggtgt agagaaagtg
    23101 tcgattctgg ggggtggact tgcaggctgg cagcgcgatg atctgctgtt agaagaaggt
    23161 gcagtagagc tgccggaagg agagtttaac gccgcgttta atcctgaagc cgtggtgaaa
    23221 gtaaccgatg tattattggc aagccatgaa aatacggcgc aaattattga tgcccgcccg
    23281 gctgcacgtt ttaacgcaga agttgatgaa cctcgcccag gtttacgtcg cggacatatt
    23341 cccggagcac tgaatgttcc gtggacggaa ctggtgcgcg aaggcgaact aaaaacgacc
    23401 gatgaactgg atgcgatatt ttttggtcgc ggcgtcagct acgacaaacc aattatcgtc
    23461 agctgcggct ctggtgtaac ggcagccgtg gttttgttag cactcgcgac gctggatgtg
    23521 ccaaacgtga aactgtacga cggcgcatgg agtgaatggg gcgcgcgggc agatttaccg
    23581 gttgagccag tgaaataagt attttacagg caataaaaaa ccgccgaatt tggcggtttt
    23641 ttattgctgg tctggttcgc ggcctttcca gcaggttgta ttaccgtagt aatgcaagcg
    23701 cgtctcagcg gagacaatac tcgccagtaa ctctcttttt gtcaagcaaa agagagttat
    23761 tattgttctg ttagtgtatt atccactgcg gccctttccg ccgtctcgca agcgggcgct
    23821 ggcttaagga aaggatgttc cgcgaccgtt agagcaagcg cgtctcagcg cttgctatcg
    23881 cggcaatatc gccagtggtg ctgtcgtgat gcggtcttcg catggaccgc acaatgaaga
    23941 tacggtgctt ctgtatcgta cttattgttt ctggtgcgct gttttcccaa gtaaataata
    24001 accggagtct ctccggcgac aatttactgg tggttaacaa ccttcagagc agcaagtaag
    24061 cccgaatgcc gccctttggg gcggcatatt ttagattatc cgattctgtt taaagtcacg
    24121 caaaaaacca ccccagcgac gttcatagaa tggcacaata tgttcggtaa taaagtggct
    24181 aattcctttt tccccttttt tcacctgaca aatatcgatt ggttcatcgc caggtaatgt
    24241 atcggtcgct acacttcccg tcgcctgaat aatttcttcg atatcaccat cggcttcaat
    24301 gccaataagt aaattaggct gtgcctcttc gttctcttta attgaacaaa taaaagcacg
    24361 cttcaccgac ttaatggttt taaataaggt ggtgagtgaa tcaatcattt gtgctggcgg
    24421 ctctgcgact tccgataata tcagcgattc accgccttcc aggatttcct ggctgctcag
    24481 cggatttccc tcttcaccaa tcaacaaact gatttcacgc ggcataaatt ctttaccggt
    24541 cggcagtttg gcattaagga agagcgtttc gccaagggtc atctcaaaca atgtgcgaac
    24601 gggcattacg acaaatgcct gttcgtcttc aaccgcctgt tgaagcgctt ctaacgaggt
    24661 gaaaaaagga atgacgctgg tgccgtcttc tttttcccag tgctgtaaat caagcgcact
    24721 atcttcaacc acagcctcgc cctgcgccgc cgtaccaggc acccagacgg tggattccag
    24781 tagagtacgg aaaaaggccg ggcggtgcgc cggttcggtt gctgcttttt ccagcaggtc
    24841 ttcaagttcg ttttttgttt cggacataag aaccacaatt cattcaacgt tcggggcgca
    24901 aatgcctgat gcgctacgct tatcaggcct acaagggatt cgcaatttgt tgaatttgca
    24961 gaatttgtag gccggataag gcgtttacgc cgcatccggc atattagttt acgccgttaa
    25021 cagattagca atcgtgcgca caccaagtcc cgtagcgccc gcagaccact gttcaaccgg
    25081 cgctttacgg taagtcgccg agcagtcgat atgcagccag ccttgctgat agttctcaac
    25141 aaagtgcgac aggaagcccg ccgccgtgct cgcgcctgcc ggatacgccg cgcttccggt
    25201 attgttcagt tcggcaaagt tagacggcag ctggctgcgg tggaattccg ccagcggcag
    25261 acgccagaac ggttcgttct cttgtgaggc actcgccagc aaacgaccgg caagcgcatc
    25321 gtcaaaactg aacagcgcgt gataatcatt acccagcgca gttttcgccg ccccggtgag
    25381 ggtcgccgca tcaatgatca gttccggttt ctgcgcactg gcatcaatca gaccatcggc
    25441 cagcaccaga cgcccttcgg catcagtgtt catcacttca acttttttac cgttgcgata
    25501 ggtgatgata tcgcccagct tgaacgcatt gccgctaatc aggttatccg cacagcagag
    25561 gaacagcttc acgcgcttgt tcagtccgcg cgtaatggca aatgccagcg ccccggtaac
    25621 cgttgccgcg ccgcccatgt ccgacttcat cgagtccata aacgcagtct gttttatgct
    25681 gtagccgccg gagtcaaaag tgataccttt acctaccagg cacgcgtaca ccggtgcttc
    25741 tttatcgcca gttgggttgt aatccagcgc cagcaatacc ggagaacgtt ctgaaccgcg
    25801 cccgacagtg tgcagcccca tataaccttg ctcacgcaga tcttcgcctt tggtgatccg
    25861 ataagtcaca cgatcgcccg cgacgttgct gatcagatca acagcacgct gtgccagttg
    25921 cgatggtccc aattcttctg ccggtgcgtt gatggtgtca cgcacccagt cgatgatcat
    25981 caggcggtta tccagttcct ggcgctgggc atcgtccaga tccggccact ccactttacg
    26041 cgtgcctttc ggggctttgt aaccttgcca gaatgcccag cagcgatccg catcccagcc
    26101 ttcaccgctt aactgaacat gcttgatgcc cagaccgtca atcttgcgcg ccgcacgctg
    26161 gatcagcccc agatcgtctg ccccgttcaa atgcagggta atgccgtcat tattaatgct
    26221 gtaagttgct ttttctcccc agcgcgcgtc ggcaggttgg gtagagaggg taatattcat
    26281 cgcttctgtc attttagtta tccttctttg taaaagggcc gccagttggc agccccagga
    26341 tttttccgct aaatgattcg cgttgcagga aggcggcaag tgagtgaagc cccaggagca
    26401 tagataacta tgtgactggg gtgaacgagc gcagccaacg catctgcggc gtgaagcatg
    26461 acgcggaaat tattcggcct cgtccagcca gactaacaaa atcgcttcga ggattttctc
    26521 gttggatgcc tgcgggtcgt cgtcgaaatc ttccaaatcg caaatccact gatgcatatc
    26581 ggtgaatcga accgttttcg gatcaagatc gggatacgca tcgtacagtg cttcgccaat
    26641 ttcgcggcta tcggtccact taagtcccat actaacctct gttaatgctc acgcgcatgg
    26701 ttgatagtgt aacgcgggat ttcgaccact aaatcttcgt cggtgacgcg cgcctggcag
    26761 cttaaacggc tttccggctc cagtccccag gctttgtcca gcatgtcgtc ttcctgctct
    26821 gagctttccg gcagcgagtc aaaaccttca cgaacgatgc agtggcaggt ggtgcaagca
    26881 caggattttt cacaggcgtg ttcaatctcg ataccgttac gcagagctgc gtcgagaatg
    26941 gtttcaccgc tattagcttc cagaacagcg ccatcagggc agagatcctg atgaggcaaa
    27001 ataacaatct ttggcatatt aaacctcgtc cacggaatgg cctttcagcg cacgacgaac
    27061 cgactggtcc atgcggcgag cggcgaaatc ctgggtttgt ttgtctacgt ttttaatcgc
    27121 ttgttcgatg gcgtcaacgt catcgccctg cgccacttca ctcaggtgag cggcagcatc
    27181 gtcaatgacc tgacgttctg cggcgcttaa cagcgcggca tcagcagcca gcgcgccgtg
    27241 cagactttcc agcacacgcg ccgcttctac tttttgttct gccagcattc gggcttttac
    27301 gtcctgctcg gcatagctca ttgagtcttt gatcatcgaa gcgatttcgc tatcggtcag
    27361 accgtaagac ggtttgacct gaatagacgc ctcaacgccg gtggatttct ccatcgccgt
    27421 cacgctcaaa agaccgtcgg catcgacctg gaacgtcacg cgaatatgcg caccaccagc
    27481 cggtagcgcc ggaataccac gcagcgcaaa acgcgccagt gagcggcagt cctgcaccag
    27541 ttcgcgctca ccctgcatta catggataga catcgccgtc tgaccatctt taaaggtggt
    27601 gaaatcctga gcgcgggcca ccggaatcgt ggtattacgc ggaatcactt tctccaccag
    27661 gccgcccatc gtttcgaggc ccagcgacag tgggatcaca tcaagcagca gcatttcgct
    27721 gtccggcttg ttacccacca gaatatccgc ctgaatcgcc gcgccaatag cgacgacttt
    27781 atccgggtcg atggaagtca gcggtggacg accgaaaaat tcgcctaccc gttcacgcac
    27841 cagcggcacg cgagtagaac cgcccaccat caccacttcc agcacttcat cagcttctac
    27901 acccgcgtct ttcagcgcgc gacgacaagc cagtaaggtt cgttttacca gtggcgcgat
    27961 cagttcattg aattgttcac ggctgatttc gccctgccag cccgcaacgt taacggtcac
    28021 ggagtccgca tcgctcagcg cgattttggc tgcaatggcg gcatccagca gttcacgctg
    28081 aacgcggtta tcgctacgat caggaatgcc cgcctgctcg cgaatgtaat ccgccagcag
    28141 atggtcgaaa tcatcgccgc cgagcgcgga atcaccgccg gttgccagca cttcaaacac
    28201 gccgcgactt aagcgcagaa tggaaatatc aaacgtcccg ccaccgaggt cataaacagc
    28261 gatcacgcct tcctgaccgg aatccagccc gtaggcgata gccgcagcgg tcggttcgtt
    28321 aagtaagcgc agaacatgca accccgccag acgcgccgcg tctttggtgc cctgacgctg
    28381 ggcatcgtca aagtacgccg gaacggtgat aactacacca tccagctcgc ctgccagggc
    28441 ttcagttgcc cgcgccgcca gtgctttgag gatgtccgca gaaacgcgca ccgggttcag
    28501 cagccccgcc gccgtttcaa tcatcggcag gccgttttcg ctggcctgga attgataagg
    28561 cagatgcgga tagcgttgct ggatatcagc cagcgagcgc cccatcaggc gtttaacaga
    28621 actaatggtg ttggcggtat caagcgctgc gttagtacgc gcgtcataac ccaccgaatg
    28681 cccttgctgt tgatagtgaa caacagatgg cagcaggtga cggccttcat ggtcggctaa
    28741 cgtttcggcc tgaccgctgc gcactgtcgc caccagcgag ttggttgtgc ccaggtcaat
    28801 accagccgcc agacgacgct gatgcggcgc agcactcaaa ccaggttcac taatttgtaa
    28861 taaggccatg tttagcttcc agaaattaaa aatcgagcag tttttcttcg agttgttcgg
    28921 cactgctccg cagtttatcg agaaaacgca gcttacgcac ggtatccgcc gccgcgtccc
    28981 acgcctcgtt gtctaactgt tcaaccatca actgatggcg ggtatcaaac atctttttca
    29041 cacgtttgat aaagctttcc agccgcgctt catctttcgc ctgttcgatc tcgtccagct
    29101 cttcgcgcag ctccaactgt tccatcagga acgcggtgtc gcgcacagta tgctgctcgc
    29161 tggcgagatc aaaaccgtgt aaagaaagca aatattccgc gcgcattaac ggatgacgca
    29221 gcgtttgcca ggcctggtta atggttgcag attgctgtac ggcggcgagt tgttccgcct
    29281 ggcttccgct ggcgaattta tcaggatgat actgacgttg tagatcctga aaacgcaggc
    29341 tcagcgcctg ggtatcgagt tgataacggg caggcaagcc aaagagggtg aagtaatcca
    29401 taacattctc agggctgcga tccgccgcga taacggcgaa tcaggtaaaa caaaccccac
    29461 gcgcaggcga ccacggtggg gttatcggta tgcgcatcaa acgtggaagc tttcgccgca
    29521 accacactca tctttgacgt tcgggttggt gaatttaaac ccttcgttca ggccttcttt
    29581 tacgaagtcc agctgcgtac cgtccagaaa ttgcaggctt ttgccatcga ccacgacttt
    29641 cacgcctttg tcttcaaaca cgatgtcttc cggcgtcggt tcgtcaacaa attccagtac
    29701 ataagccata cctgaacatc cggaggttct cacgcccaga cgcaggccaa accctttgcc
    29761 gcggttagcc agaaaggtat ttactcgcgc tgctgcactg tcgctcagtg taatcgacat
    29821 aaccaaacct caactcttat tttgcttcac gtttgctttt atagtccgca atggcggctt
    29881 tgatcgcgtc ttctgccaga atagaacagt gaattttcac cggcggcagt tcaagttctt
    29941 cagcaatatc ggtatttttg atcgcctgcg cttcgtcgag agacttccct ttcacccatt
    30001 cggtgaccag ggagctggaa gcgatagcag aaccgcagcc gtaagtttta aaacgcgcgt
    30061 cttcaatgat accttcatcg ttgactttaa tctgcaactt catcacgtcg ccacaggccg
    30121 gtgcccccac catgccgctg ccgacgttct cgtcgttgtt gtcaaaggaa cccacgttac
    30181 gcggattctc gtaatggtcg ataacttttt cgctgtaagc cattataaat tctcctgatt
    30241 ccgataccga ttaatgatga gcccattcga tgctgttcag atccacgccc tgcttgtaca
    30301 tttcccacag cggagaaagg tcacgcagac gaccgatgga tttacgaact aactcgatgg
    30361 tgtagtcgat ctcttcttca gtagtaaaac gacctaaaga gaaacggata gagctatgtg
    30421 ccagctcgtc gttcagcccc agcgcgcgca gcacgtagga cggttcgagg cttgctgacg
    30481 tacaggcgga acctgaagaa actgcgaggt ctttcagcgc cataatcagc gactcacctt
    30541 caacgtagtt gaagctgacg ttgagaatgt tcggcgcacc gtgttccagg tcaccgttca
    30601 ggtaaacttc ttcgatatct ttgatgccgt tccacagacg gttacgcagg ccgcgcagac
    30661 gttccatctc ggtcgccatc tcttcttttg cgatgcgata ggcctcgccc attccgacga
    30721 tctggtgaac aggcagagtg ccggaacgca taccgcgctc gtgaccgccg ccgtgcattt
    30781 gcgcttcgat gcgtacgcgc ggtttacgac gtacatacag cgcaccgata cctttcgggc
    30841 catagatttt gtgaccggag aaagacatca ggtcaacttt caactggctc aggtcgatag
    30901 gcagtttgcc cacgctctgg gttgcatcaa cgtgatagat aatgccacga gcacggcaca
    30961 tttcgccgat agccgcgata tcctgcacca cgccgatttc gttatttacg tgcatgatgg
    31021 aaacaaggat ggtgtcgtca cgcatcgctg cttcaagttc tttcaggtcg ataatgccgt
    31081 tacgctgcgg tgccaggtag gtgacttcaa aaccttcgcg ctccagctga cggcaggtat
    31141 ccagtaccgc tttgtgttcg gttttgctgg tgatgatgtg cttgcctttt ttctgataaa
    31201 agttggctgc acctttgatc gccaggttgt cagattcggt tgcaccagag gtaaagacga
    31261 tttcacgcgg atcagcgccg accagatcgg caatctgatt acgggcgata tctaccgctt
    31321 cttcagcctg ccagccgaaa cggtgagaac gagaggccgg gttaccaaag gttccgtcca
    31381 tcgtcataaa ctgcatcatt ttctcggcaa cacgcgggtc caccggcgtg gttgcggagt
    31441 agtcgagata aatcggtaat ttcattgctc tataaactcc gtacatcact caatgcaagg
    31501 aaccaggcta ccggctggat gtacgaccgt gtttacggag cgcgatacgc cccggcctga
    31561 ttctgaattc tttttattaa gcgcgtaact taacgtcgat cgcgtcttgt atgcgggtgc
    31621 gtggcgcgtc gtgagtatgc tgacgaccag acacatccag cacttcctgg ttattaacca
    31681 gttcgcctaa agtaatgttg ttgagaaaac cggtgagacg gtcgctcaaa tcacgccaca
    31741 gcgcgtgggt caggcattta tcgccgccct ggcagccgcc tttaccctga caacgggtgg
    31801 catctacaga ttcgtcaacg gcgctaatca cttcgccaac ggcgatgctg ctggcatctt
    31861 tgcctaacag ataaccaccg cctggtccac gtacgctgga aaccagacca tttttacgca
    31921 gacgggaaaa cagttgttcc agataagaaa gggaaattcc ctgacgttcg gaaatatcag
    31981 ccaacggtac cgggcccgct tcagagttga gcgcaacgtc aagcattgcg gtcacggcat
    32041 agcgcccttt agatgtcagt ctcatgtctt acttcacctc aaactcgccc ctgcccgggg
    32101 ttttttattg taaaagtggg ggtattgcat agcagggtca agtctgacat tcccgagtaa
    32161 attggtcaac tatttacttg actgatttag tcgggtattt aaccttcagt gccatttttt
    32221 tatcgtggta tttgtacctg ttgtcggatg cggcgtgaac gccttatccg acctacgatt
    32281 gcgtgcactt ccagcacttg taggcctgat aagacgcgtt aagcgtcgca tcaggcaacc
    32341 actgttattc ggccttatta cctttattct gctgctctat agaagccaga atcccgcgca
    32401 ggatattcaa ttcctggctt tccgggcgcg cacgggtaaa cagacggcgc aatttattca
    32461 tcacctgccc cggatggttt tcacggataa aaccggttgc cagcagcgtt tgttcgagat
    32521 gaccataaaa acgctccaga tcatcgacca gcggatacgg cgtctcttca tgctcgacct
    32581 gctcgccatt ttcctgagtc gccagccagg ccatacgcac ttcataggca ataacctgaa
    32641 ccgccatcgc caggttcagc gagctgtatt ccgggttcgc ggcaatcgcg acatgataat
    32701 ggcatttctg caactcttca ttggtcaagc cgacgcgctc acgaccaaac accagcgcca
    32761 ccggggtatt tgccgcttca gcgacgcttt tcagaccgca ttcgcgcggg tcgagcatcg
    32821 gccacggcag cgtgcgggag cgtgcgctgg tgcccaccac caggctacaa ccggctaacg
    32881 cttcgtcgag cgtatcgacg atgtgagcat taccaatcac atcgctggcc cctgctgcca
    32941 gggcaatcgc ctgggagtcg ggtttcacca gtggattaac cagccacaga ttggttaatc
    33001 ccattgtttt catggcacgg gcaacagaac ccatattgcc ggtgtgtgac gtctccacca
    33061 gcacaattcg aatattttgc agcattgtct ttcttcatct aaagattatt cacgcatctt
    33121 atcataaaac gaagacagat gccgatctcg ctgctatact ctgcgccgtt ttcccgttct
    33181 ttaacatcca gtgagagaga ccgatgcatc cgatgctgaa catcgccgtg cgcgcagcgc
    33241 gcaaggcggg taatttaatt gccaaaaact atgaaacccc ggacgctgta gaagcgagcc
    33301 agaaaggcag taacgatttc gtgaccaacg tagataaagc tgccgaagcg gtgattatcg
    33361 acacgattcg taaatcttac ccacagcaca ccatcatcac cgaagaaagc ggtgaacttg
    33421 aaggtactga tcaggatgtt caatgggtta tcgatccact ggatggtact accaacttta
    33481 tcaaacgtct gccgcacttc gcggtatcta tcgccgttcg tatcaaaggc cgcaccgaag
    33541 ttgctgtggt atacgatcct atgcgtaacg aactgttcac cgccactcgc ggtcagggcg
    33601 cacagctgaa cggctaccgt ctgcgcggta gcaccgctcg cgatctcgac ggtaccattc
    33661 tggcgaccgg cttcccgttc aaagcaaaac agtacgccac tacctacatc aacatcgtcg
    33721 gcaaactgtt caacgaatgt gcagacttcc gtcgtaccgg ttctgcggcg ctggatctgg
    33781 cttacgtcgc tgcgggtcgt gttgacggtt tctttgaaat cggtctgcgt ccgtgggatt
    33841 tcgcggcagg cgagctgctg gttcgtgaag cgggcggcat cgtcagcgac ttcaccggtg
    33901 gtcataacta tatgctgacc ggtaacatcg ttgctggtaa cccacgtgtt gtgaaagcca
    33961 tgctggcgaa catgcgtgac gagttaagcg acgctctgaa gcgttaatga ctcaggcggg
    34021 tgatgtcact cacccgccct cgcctttcag gcgctattct gaaatacttc ctcaccgctt
    34081 tactttcttt cgtcactctc ccaccatctt tccccgatta atggatgata aagaagtcgc
    34141 aaggaaaggt tatggcactg ccagtgaaca aacgcgttcc caaaattctg tttattctct
    34201 ttgttgttgc cttctgcgtt tatttagtgc cgcgcgttgc catcaacttc ttctattatc
    34261 ctgacgataa aatttacggt cccgacccct ggtcggcgga atccgtcgaa tttacggcta
    34321 aggacggtac tcgtctgcaa ggctggttta ttccttcttc gacgggccct gctgacaacg
    34381 ccatcgcaac catcattcat gctcacggca atgccggaaa tatgtccgcc cactggccgc
    34441 tggtcagttg gttacctgag cgtaatttca acgtttttat gtttgattat cgcgggtttg
    34501 gtaaatcaaa aggcacgccg tcccaagccg gattgctgga cgatacgcaa agtgccatca
    34561 atgtggtgcg ccatcgcagt gatgtaaacc cacaacgtct ggtgctgttc gggcagagta
    34621 ttggcggggc gaatattctg gatgttattg gtcggggtga tcgtgaaggc atacgtgcgg
    34681 tgatcctcga ctccacattt gcctcttatg caaccatcgc caaccagatg atccccggca
    34741 gtggctactt acttgatgag agttacagcg gcgaaaatta tatcgccagc gtcagcccga
    34801 tcccgctttt actcattcac ggtaaagctg atcacgttat cccatggcag cacagcgaaa
    34861 agttgtatag cctggcaaaa gagccaaaac ggttgatcct aatcccggat ggcgaacaca
    34921 ttgatgcttt ttccgatcgt cacggcgatg tttatcgcga acagatggtg gactttatcc
    34981 ttagcgcgtt gaatccacag aactaaccca tgatcgctag cacgataatc attcacaaaa
    35041 ccaccttaag acatgctaat ccactggtca gaacagttta agatgagaaa aattctgtga
    35101 cgcttgccaa catttctgat gattagcatt cccttcgcca tttccctgag caaactttag
    35161 ctattcttat caattatgct tatgggagat ctacagatga tgcctacgct tgctccacca
    35221 tctgtccttt cggctcccca gcgccgctgc cagatcttgc tgacgctctt tcagccgggg
    35281 ttgaccgcca ccacggcaac tttcagcgag cttaatggtg tggatgatga tattgccagt
    35341 cttgatatca gcgaaacagg acaggagatc ctgcgctatc atcaactcac actgacggct
    35401 ggttatgacg gtagctaccg ggttgaaggt acagtgctta accaacgttt gtgtttattt
    35461 cactggctac gacgtggttt ccgtctgtgt ccatcattta ttaccagcca tttcaccccc
    35521 gccctgaaga gtgaactgaa gcggcgcgga attgcgcgta acttttacga cgataccaat
    35581 ctacaagcgt tagtgaatct ctgctcccga cgactgcaaa aacgctttga aacgcgcgat
    35641 attcatttcc tgtgtctgta tctgcaatat tgtttgctgc aacaccacgc tggaattacg
    35701 cctcagttta atccgctcca acgtcgctgg gcggaatcct gccttgaatt tcaggtagcg
    35761 caggaaattg gacgccactg gcagcgtcgg gcgctccagc ctgtaccgcc tgatgagcca
    35821 ctgtttatgg cattactttt ttccatgttg cgggttcccg atccattgcg tgatgcgcat
    35881 cagcgagaca gacaattgcg tcagtctatc aaacgtctgg taaaccattt tcgtgagctg
    35941 ggaaatgttc gtttttatga tgaacagggg ttatgcgatc agctttatac ccacctcgcc
    36001 caggcgttaa atcgcagttt ttttgccatc ggtattgata ataccctgcc ggaagagttc
    36061 gccagactgt acccacgcct ggtgcgcacc acccgcgcgg cgctggccgg atttgaaagt
    36121 gaatacggcg tccatctttc tgatgaggaa agtggtctgg tcgcggtgat tttcggtgcc
    36181 tggctaatgc aggaaaacga tctgcatgaa aaacagatta ttctactgac cgggaatgat
    36241 agcgagcgag aagcgcagat tgagcagcag ttacgcgaac taacgttact gccgctcaac
    36301 attaagcata tgtcggtaaa ggcatttttg cagacaggcg ctccgcgtgg cgcggcactg
    36361 attattgcgc cttataccat gccattaccg ctcttttcac caccgctgat ctatacggac
    36421 ctgacgctga caacacatca acaggagcag atccgcaaaa tgctcgaatc agcatgaggg
    36481 aacaactttc gggcgcaaaa acattgccgg cagcgccacc agcgccatta cccagaacac
    36541 gccgtggccc agatattgat acaggaaacc ggcgaaaacg gtcatgatag cgatactgcc
    36601 gcccatcgcg acggcagagt aaaccgcctg taaacggatg acttcgctac cctggcgagc
    36661 agcaatataa cgcatggcgg ccaggtggca gaccgtgaag gtgccgcaat gcagaatttg
    36721 caccactatc aaccacggca acgccgtagt tgctcccata atgccccagc gcactacgcc
    36781 gcaaatcgcc gagatcaaca gcatatcgcg tgcactacaa cggcggaaaa gtttattact
    36841 cagcgcaaag ataatgactt ccgccaccac gcccagcgac cacaaatacc ccaccgccga
    36901 ggccgagtag ccagctgcct gccagtaaat ggcgctaaaa ccgtaatagg ccgcatgtgc
    36961 cccctgcaat aaacaaacgc aggccagaaa gcgccagttc tggcgaacca gcgccaacca
    37021 cgctgaccaa ccggtgctct cctgctggcg gcttgcccct tgtggctgaa tcgtcggacg
    37081 gatgagaaag ccgagcagca tggatgccac gcccaacgtc aacagcgcga ggatcacccg
    37141 ataatcaaac atagtgacca gtttgcccgt cagcgccgag ccaatgacaa acgccaccga
    37201 gccccacagt cgcactttgc cgtaatcaag cgggaactgc ttttgccacg tattcgccag
    37261 tgcatcggtc aacggtacca gcggtgagaa aaagaggtta aagccaatca tcaccagcat
    37321 cagccacgct acgtgcgccc ccgcccagaa ggcgacagca aagagaagtg tcagcagtgc
    37381 cagcacgcgc aaggcggaaa tcaggcggga aggatcgctg acgcggggcg cgatgagcaa
    37441 actcccgagg aaacgggcaa ccagacctgc ccccaataac aggccgatgg tttctggcgt
    37501 taaaccaatc cctttaagcc agacgctcca gaaaggtaga aaaatgccgt aactaaaaaa
    37561 gtatgtgaaa tagccgagcg ccaaccagcg cgtggattgc aaaaccatga gtccctcccg
    37621 tcaggaggcg ttagtctggg gttaatcgaa tgttggtgca agttgaaaaa taagaatatt
    37681 cgagcctgct tcccgatgtc tgtaacgcca gttcacaagc actaaaaaac tgagatccgc
    37741 gacgcatgtt gcaaaaataa tgcgtaatta tgccgttacg cttgccaaac gttcctgcac
    37801 aatggcgatg aaatcgcgta atgccggctt catctcccct ttcttccacg ccatcagtaa
    37861 agcaatggaa ggcacatttc cggcaatggg acgaaaaaca acctgtccgg tattaaaatt
    37921 attcatataa ccgggtatca aagtgacgcc cagccccatg cccaccagat tcatggtcac
    37981 cagaatattc gttgccacct ggacgatatt tggctggctg ttttcttgcg caaaccacgc
    38041 tttaacgatc ggcgcaagcg aaccggaata caccggatcg gtactgacga aattcacgcc
    38101 atccagttgc gcggcggtga tctctttttc atgtgctaaa gggtgatcaa ccggtaacac
    38161 aaccactaat ggttcgtcaa aaagctccag ataatcaatc tccgggctat aaacaggatg
    38221 gcgcattaag ccgacgtcga gttcaccacg gcgaattttt tcctcctgtt gcgtggtgat
    38281 taaactcacc agctcaatca aggtgtctgg ctgtctgaga cgaaacatcg gtaatacttt
    38341 tggcagtaaa ttcacttccg ccgatggcac aaagccaatg gttaattgtc tgtcttcctg
    38401 aacaattttc cgcgcccgta atttggcatt ttccgcttgt tcgaggattg ccagcgcgtc
    38461 ctggagaaaa cattctcccg ccgccgtcaa cgcgactttg cgcttatccc tcaccagtaa
    38521 cggaacaccg acacagtttt caagatcgcg gatctggctg cttaacgaag gctgtgaggt
    38581 atgcagtttt tccgccgcac gggtaaagtt cagtgcctgc gccactgcga cgaaatagcg
    38641 taaatgccgt agttccatca ccttcccctt gttatcgaaa aaacgtctca gctggtagaa
    38701 attaactatt tcacatatta gcaaccaacc agcaacatcc ttatggcaca aaaatagaag
    38761 gtcaatacat cttatctttc aggattaaaa aatatgacca caccctcaga tttgaacatt
    38821 taccaactga ttgacaccca aaatggtcgg gtcactccgc gtatttatac cgacccggat
    38881 atttaccaac tggagcttga gcgtattttc ggtcgttgct ggctatttct cgcccacgaa
    38941 agccagatcc caaaacccgg tgatttcttt aacacctaca tgggagaaga tgcggttgtc
    39001 gtagtgcgtc agaaagacgg cagcatcaag gcgtttctca accaatgccg ccaccgggcc
    39061 atgcgtgtga gttatgcaga ttgcggcaac actcgcgcct ttacctgccc gtatcacggc
    39121 tggtcttatg gcattaacgg cgagttgatc gatgtaccgc tggaacctcg cgcctaccca
    39181 caagggttgt gtaaatccca ctggggacta aacgaagttc cttgtgtgga gagttataaa
    39241 gggctgattt ttggcaactg ggataccagc gcaccgggcc tgcgtgatta cctgggtgac
    39301 attgcctggt atctggatgg catgctggat cgtcgcgaag gcggcaccga aattgtcggc
    39361 ggcgtacaaa agtgggtgat caactgtaac tggaaattcc cggcagagca gttcgccagt
    39421 gaccagtatc atgctctgtt cagccatgct tctgccgttc aggtattagg ggcgaaagat
    39481 gatggcagcg ataagcgcct cggtgatgga caaaccgccc gcccggtgtg ggaaaccgcc
    39541 aaagatgcgc tgcaatttgg tcaggacggt cacggtagcg gtttcttctt tactgaaaaa
    39601 ccggatgcta atgtctgggt cgatggcgca gtttcaagct attaccgcga aacctatgcc
    39661 gaagcagaac aacgtttagg tgaagttcgc gccctgcgcc tggcgggtca taacaatata
    39721 ttccccacgc tttcatggct aaacggcact gccacactcc gcgtctggca tccgcgcggc
    39781 cctgatcaag ttgaagtgtg ggcgttctgt attactgaca aagccgcctc cgatgaagtt
    39841 aaagccgctt ttgaaaacag cgccactcgt gcttttggtc ctgctggttt tctcgagcag
    39901 gatgactcgg agaactggtg tgaaatccag aaattgctta aaggccaccg cgcccgcaac
    39961 agcaaactgt gtctggaaat ggggcttggt caggaaaagc gccgcgacga cggcattcct
    40021 ggcattacta actatatctt ttcagaaact gccgctcgtg gaatgtacca acgctgggcc
    40081 gatcttctga gtagcgaaag ctggcaggaa gtgctcgata aaaccgccgc ttaccagcag
    40141 gaggtgatga aatgagtgcg caagtttcac tagagttaca tcaccgcatt agccagtttc
    40201 tctttcacga agccagctta ctggacgact ggaaatttcg tgactggctg gcgcagctcg
    40261 acgaagagat tcgttacacc atgcgcacca cagttaacgc gcaaacacgc gaccgccgca
    40321 aaggcgtcca gccaccgaca acctggattt ttaatgacac caaagaccag ctggagcggc
    40381 gaatcgcccg tctggaaacg ggcatggcat gggcagaaga gccgccgtca cgcacccgcc
    40441 acttaatcag caactgccag ataagcgaaa ccgacatccc aaacgtattt gctgttcggg
    40501 tgaattatct gctttatcgg gcacaaaaag agcgcgatga aacgttctat gtcgggactc
    40561 gttttgacaa agttcgccgt ctggaagatg acaactggcg cttgctggaa cgggatatcg
    40621 tcctggatca agcggtaatc acttcccata acctgagtgt actgttctga tgaatcgaat
    40681 ttatgcgtgt cccgttgcgg atgcgccgga gggtgaggct ctccggatcg atacctcgcc
    40741 cgtcatcgcc ctgttcaacg ttggcggcga gttttatgcc attaacgatc gttgcagcca
    40801 tggtaatgcg tcaatgtcag aagggtatct ggaagatgac gccacggtgg agtgcccgct
    40861 acacgccgcc agtttttgcc tgaaaacggg gaaagcgtta tgcctgcccg ccaccgatcc
    40921 gctcaccact tatccagtac acgttgaagg tggtgacatt ttcatcgact taccggaggc
    40981 gcagccatga gcgatctgca taacgagtcc atttttatta ccggcggcgg atcgggatta
    41041 gggctggcgc tggtcgagcg atttatcgaa gaaggcgcgc aggttgccac gctggaactg
    41101 tcggcggcaa aagtcgccag tctgcgtcag cgatttggcg aacatattct ggcggtggaa
    41161 ggtaacgtga cctgttatgc cgattatcaa cgcgcggtcg atcagatcct gactcgttcc
    41221 ggcaagctgg attgttttat cggcaatgca ggcatctggg atcacaatgt ctcactggtt
    41281 aatactcccg cagagacgct cgaaaccggc ttccacgagc tgtttaacgt caatgttctc
    41341 ggttacctgc tgggcgcaaa agcctgcgct ccggcgttaa tcgccagtga aggcagcatg
    41401 attttcacac tgtcaaatgc cgcctggtat cctggcggcg gtggcccgct gtacaccgcc
    41461 agtaaacatg ccgcaaccgg acttattcgc caactggctt atgaactggc accgaaagtg
    41521 cgggtgaatg gcgtcggccc gtgtggtatg gccagcgacc tgcgcggccc acaggcgctc
    41581 gggcaaagtg aaacctcgat aatgcagtct ctgacgccgg agaaaattgc cgccatttta
    41641 ccgctgcaat ttttcccgca accggcggat tttacgggtc cgtatgtgat gttggcatcg
    41701 cggcgcaata atcgcgcatt aagcggtgtg atgatcaacg ctgatgcggg tttagcgatt
    41761 cgcggcattc gccacgtagc ggctgggctg gatctttaag gaagcacgat gaaagaaaaa
    41821 accatcatta ttgtcggtgg cgggcaagcg gcggcaatgg ctgcggcctc gctacgccag
    41881 caagggttca ccggtgagct gcatctgttt tccgatgagc gacatcttcc ttatgaacga
    41941 cctccactct cgaaatccat gttgctggaa gattccccgc agttacagca ggtgttaccc
    42001 gctaactggt ggcaggaaaa caatgttcat ctgcattccg gtgtaaccat caaaacgctg
    42061 ggtcgcgaca cacgagagtt agtgttaacc aacggcgaaa gctggcactg ggatcagctt
    42121 tttatagcaa ccggcgcggc agctcgaccg ctgccgttgc ttgatgcact gggagaacgc
    42181 tgctttaccc tacgccatgc cggtgatgcc gccagactgc gagaagttct gcagcccgaa
    42241 cggtcagtcg tgattgtcgg tgccggaact attggtctgg aactggctgc cagcgccacg
    42301 cagcgcagat gtaaggtgac agtgattgaa ctggcggcaa ccgtcatggg ccgtaatgca
    42361 ccaccgcccg tgcaacgcta tcttttacag cgccaccagc aggctggtgt gcgcattctg
    42421 ctcaataatg ccattgaaca tgttgtcgat ggtgaaaaag tagaactgac gctgcaaagt
    42481 ggggaaacgc ttcaggctga tgtggtgatt tacggtattg gtatcagcgc caacgagcaa
    42541 ctggctcgcg aggccaacct tgatactgcc aatggcattg tcattgatga ggcttgccgc
    42601 acctgcgatc ccgcgatctt tgccggtggc gatgtggcaa tcactcgtct tgataatggt
    42661 gcactacacc gctgcgaaag ctgggaaaac gccaataacc aggcgcaaat tgccgctgcc
    42721 gcaatgttag ggctaccgct accgctactg ccgccgccgt ggttctggag cgatcagtac
    42781 agtgataact tacagtttat tggcgatatg cgtggcgatg actggctttg tcgtggcaac
    42841 ccggaaactc agaaggcgat ttggtttaat ctgcaaaacg gcgtgcttat cggtgcggtc
    42901 acgctgaatc aggggcgtga gattcgccca attcgcaaat ggatccagag cggcaaaacg
    42961 tttgatgcga aactgctgat agatgagaac atcgcgctta aatcactgta accaggataa
    43021 ttagcgaata tctcaatgcc tggggcgtgg cgaggtgcaa gagtgtgtat tacgtttaaa
    43081 tcacattatc ttgcaaaggg attggttatg aacacactac gttattttga ttttggagct
    43141 gcccgccccg ttttgttatt aattgcccgt atcgccgtgg tcttaatttt cattattttt
    43201 ggttttccca aaatgatggg ctttgacggt acggtccaat atatggcctc gttgggcgcg
    43261 ccaatgccga tgctggcagc gattattgcg gtagttatgg aagtgcccgc cgcgatatta
    43321 atcgtgcttg gctttttcac ccgtccgctg gcggtgctgt ttattttcta cacgctgggt
    43381 acggcggtga ttggtcacca ttactgggat atgaccggcg atgcggttgg gccaaatatg
    43441 attaatttct ggaagaatgt cagtatcgct ggcgcattcc tgctgctggc aattaccggg
    43501 ccgggggcaa tttctctcga tcggcgttag gaaagatgcc ggatgcggcg tgaacgcctt
    43561 atccggcatt taataaatta cagccactct acccgcaacg acatctctga agttgttgat
    43621 tcccctggtg ctagcgcaat gagatcaccc ccttccgggc gatgatgatc atccggcgca
    43681 tggctcatcg gttccagaca gaagaaatca aacgcatatc ctttatcaaa cgcagggtcg
    43741 gaaacaaaga tgaaataaca cggtgcaggt ggcgtcgttt ccatgatgat ggcataacct
    43801 tcctgcggct gctcgatgcg ggcctgacca ttccatccgg caaaaccatt gttcacccac
    43861 tggcgcggca acggcgcggg ctggttaaaa tccagttcct gcggtagctg ctcgcaaaac
    43921 tcacccgcca gccactgctc ccgctccagc cagtaaccgc tcgcctgtgc ctgaatccgc
    43981 gtttgcggcg acaacggaaa ataaggatgc cagccggtac caaatggcag cgtctctgcc
    44041 ccttgattgg tgacagagag cgtcaccgtc agcgtatccg ccgttaaatg aaacgcctga
    44101 cttacccgat agtgatagac accgctgcga tgttcataca ccagacacaa actatcatcg
    44161 ctgtgcgaga cacattgcca ctcgcccagc cagccatcgc cgtgtagata gtgcgcatcc
    44221 cactcaacat tcggttgcag ttgatactca cgcccctgcc agacaaaacg attgccgctc
    44281 acccggtttg caaatggcac cagcggaaaa catgaggcat cggttgccac accgcttttt
    44341 ttaccaggac gtaagagcgg cgtcgtatcg cgccagaagc cttcgattac gccgccctgg
    44401 tcagaaacgt ccagctttag cgacccgtgg gataaggtat agatggtcat ccgctaactc
    44461 cttaatccgg ggagttaatc acaactttcc cgcatttgcc gctcgccatc agcgcatagg
    44521 catcacctgc ctgttccagc gagaagcgat gggtaatggc gttacgcggc cacagcttcc
    44581 agtccgtcag atcatgggcg catttttcca tatggaacag actggtcacc caggagccga
    44641 taatccgccg ttgatggtgc atcagatcgg cgctgacctc gaattccact tttccggttt
    44701 caccaatgta aaccacccgt ccccagtcag cggtggattg cagtgccagc aagcgacctg
    44761 cggcattacc ggaacaatcg agcgcaacat ccgcgccacc gtgggtgagt tcggcgataa
    44821 tctgcggcag accttcggtg gttgctaaat agccgtgatc catcacccct aactgttttg
    44881 ccatcgccag acgttccggc agcatatcaa cgccgatgat ccgttttgca ccgcgacctt
    44941 tcgccagcat catcgccatc atgccgaccg gccccagacc gaccaccagc actttatcgc
    45001 tgccggaaac ttcgccgcgc aaaatccctt cataggccgt gccaacgccg caactgataa
    45061 acgcaccatc ttcgtagctc agcgcatccg gcaggaggat cagatctttt tcttccgcca
    45121 gcagatattc ggcatgaccg ccgtcacgct gccagccgta agccgctttt cctttgccag
    45181 tacaagagat aggaaagccg cggcggcagt tcgggcaaaa accacagcca gaaatgtgat
    45241 acaccagcac gcgatcaccc tctttaaaat ggcgacagcc ttgccccatc gccacaatct
    45301 gcccgcacgg ttcatgaccg ttgataaatc cctggtataa cggtttatcg ggtgccgccg
    45361 ccgtggcacg gtgttgatga tagatatagt ggacatcgct tccgcaaatc ccggaggatt
    45421 tcattttgat cagtacctgg ttaatccccg gcgtcggcac cgcaacttcc cgcagatcga
    45481 cggtcgaatt tcctggtaaa taagctgcca gcatcgtttt cataaatccc tcattaacaa
    45541 tacgattaat tttcatccct gccgcacccg cgccaggggc gttaaggttt agcgtttcgc
    45601 tttactgcta cgctgggtca gcaagatatt cgccagcacc gccaccacga tgatgacgcc
    45661 gcgcaccacc tgctggaaaa aggagttaat cccgagcagc accagaccgt taccgattag
    45721 cgtaatcacc agcacaccaa gcaatgtacc gaacagggaa ccgcgaccgc cggaaagtgc
    45781 cgtaccgccg accacgaccg cggcgatgac gtcaaactcc agaccgtttg cggcacctgc
    45841 gttaccagaa ccgaggcgcg ccgccaacaa aatgccggtc accgccgcta ataatcccga
    45901 aagggtaaag ataagaatgc gcacccgacg aacgttgatg ccgcacaact gcgccgccgt
    45961 agcattaccg ccaacggcaa aaaccgagcg cccgaaggcg gttttgcggc tgatgaacac
    46021 aaacagcgca aacaacacaa tcatgatcag cgcggatacc ggcacaccga gaaattgtcc
    46081 gcccagccag tccagcacct cgttttcgtc aatcggcact ggcagtgcgt tcgtcataaa
    46141 cagccccatt ccgcgcaggg cgctccacag ccccagtgtg gcaacgaaac ttggtacgtt
    46201 aaacacgccg cgcagcaccc cggccagcgt tcccatcagc gcgcctaaca gcaacaccag
    46261 cagacacgcc accgccagcg gaacttcaaa ttgcagcaaa aatgccaggc acaccgagac
    46321 aaaagccacc atcggcccaa cgctgacatc aatttcaccg gagataataa tcagcgtcat
    46381 cgcccaggcg gcaatcccaa tggtggcggc atcgcgcagc acgttcatct ggttattcaa
    46441 tgagataaag ccaggcgcgt tcagggagaa gaccagataa agaatggcga ttaccaccag
    46501 caaaccgatc tcattaatat ggcgactgac aaattgtttg agcgagacgc tcttgccctg
    46561 cggcaatgat aatgacgaag cagacatctt caggttcctc ggtaaaattc atcagtgcac
    46621 agacagaatg gcggacatca gctcatccac attgaccggc gagtgaaact cctgcgagaa
    46681 cgtaccgtgc tgtaacaaca ggatgcggtc gcacaccagc ggcaactcct ccacttcact
    46741 ggagataaac accacgcttt ttccttcggc agccagctca cggacaatac ggtaaatctg
    46801 ctgtttggct tcgatatcga cgccgcgcgt tggctcgtcg agcaacaaaa tctggctggc
    46861 ggcatagacc caacgaccga tcaccacttt ttgctgattg ccgccagaaa gcgtgccgat
    46921 gggcgtttcg ctactggcgg ccttgaccgt catccgctgc atcacctctt cggtcaggcg
    46981 gcggatggtg gaccattgca gtacgccgtt ggcgctgatt ttttgccgat tggtcagcac
    47041 tgtattttcg tcaacgccca accagggaat gatccccgct tctttgcggt tttctggcgt
    47101 atagccaatg ccgcgtttca gcatgtcgcc gtaatcgggg cgcgtgattt tctcgccgtt
    47161 gataacaatt tcgccctgtt catactcctc cagcccaaca atcgccttca gcaattcact
    47221 gcgccctgcc cccagcaggc cagcaatgcc aagcacttcg ccgcgacgta acgtaaagct
    47281 gatatcctcc agcttgggct tatggcgtaa tgcacggact tccagcacgg cctgatcgac
    47341 aatttcctga ggggctaccg gcgcaatatc aacgtgatcg cgccctagca ttagcgacac
    47401 aatatgatgc gtggaggtgt tttcgagcat cacatcgccc gccacctgac catcgcgcat
    47461 aacggtggca caggaggcaa tgcggcgaat ttcttccatc cggtggctga cataaatcac
    47521 cgccacgccc agtgccgaca tctttttcac cgcgctgatc accagttcaa cttccgcact
    47581 ggcaagcgaa ctcgtaggtt catcaagaat gaccacgcgc ggctcgccct tcatcacccg
    47641 tgcaatttcc accagttgct tttgcgccgg gcttagcgtt gaaacaagtt gttcaggact
    47701 aacgtcaacg cccagcgcct gtaagcaacg ttgggcatcc tgcgccattt gcaggtaatc
    47761 aatcatgccg ttgcggcgag gccattgacc aaggcagagg ttttctgcca ccgtcagccc
    47821 ttccaccaga cttaattcct gataaaccgc acgaaccccc agttcagcgg cacggcgagt
    47881 caacgtagct tcgtcacctt ccagtcgcgt ctcgccaatc cagatatcac cgctatccgg
    47941 gcgttcgctg ccggtaagca ttcgaatgag agtcgatttg cccgcgccgt ttttacctaa
    48001 cagcgcacga acttcgcctt tattgagcgt gaagttaacg ttatccagcg caacgacgcc
    48061 ggggtaacgc ttatttcctg ccaccacttt tgctaccggg actgcctctg ttgccgtgaa
    48121 catagggacc tctgcgaatc agcgattagg gcagaccatc aacgtgcgtt gccagccact
    48181 gtttaccatc ttccgttttg gtatagagat cgattggcac ctgaatcact ttttcaccgt
    48241 cggcttgttt attgataacc ttcaatgttt gcgcgagaac agcattgccc attttcttac
    48301 cggaaatatc cactaccgct ttcagcacct gattgttttc cagctcctga gcaatttcag
    48361 tggtcatatc cgaaccgaaa acagcaattt ttccggcctg attttgatta cgtaccgctt
    48421 ttaccgcgcc gagtgtcgca ccgcccgact cccccataat ggcgttgaga tccggcgtgg
    48481 agataatcag tttttcacca acggaaatcg ctttatctaa aacagtccct tcctgattag
    48541 cgacaatttg cgcgccggga acgcgggatt ttaatacttc ttcaaatcct ttacgtcgct
    48601 gcacacaaac ttcaaaggct tcgcaattga tgacggcaat tttcggctgg tcaattttat
    48661 tggcaataaa ataatcggcg gcagcgttac ccagtttttt accaaattcc agcggatcgc
    48721 cgaccagata cgccgagaca tatttatcgc cccccttttg attaatacag gtgttgtagc
    48781 aaatcaccgg aatacccgct tcactggcgc gacgaacggt acggctactg ccattttcag
    48841 acactgccga taaaataatg gcatcgacat tacgcgccac gagggtatca acaaaggtac
    48901 tttctttcga aatatcaccc tgggcgttag tttcaattaa ctgtacctgt actgaagaat
    48961 cttttgccgc atcctgaacg ccctgacgca ctccagcgta atatccctga gtatcaaggt
    49021 atattgcgcc aatggtcatt tctttttccg cagccctggc aaatagtgcg ctacctaata
    49081 gcgtagccat taataataga ttacgggttg ttctgatttt tgtaggcata gagcctcctg
    49141 tagggttttt attaacaacg gcttattcta attattttgt gatgagcggc agcgcgtgcc
    49201 gctcaaatat tacagttaat agggtttagt gaacgtagtt aaaaataaaa ggcatcacgg
    49261 tggtgaataa tgccgctttc ggccaggccg gattaatcgc cgtcaattca gcgcgagcag
    49321 actcatattg cgcaacctcc ccgagtcccg cggacgccag catcgccacc atcaggcatt
    49381 tttctttatg ctgctgttgt aaatcgccat acagcgataa caggtcaggt tgtgaaacag
    49441 caaagaaatc cgcctcaata ctggttttcg ccatctcttg cgcccactgt ttcatttcac
    49501 taaacagttg ctgtgcggtg tgttgttcgc ccagcagtcg cagcgccatt ccttgccaga
    49561 agagataatc aaccggctga tcgttgtaat aactgtggat gttaatggtg cgatcgccgg
    49621 tcgccgccag acgtaaacaa cgcatcgctt cagtttcatc gccctgcgcg ttggcgcata
    49681 tcgcctgcca gaaccagatg tcgttatcag tttgccccgg taaacggcct tcgcttaaat
    49741 tctccggata atgcagcgcg gcatgaagca gttcgctggc ctgctgcggc tgtctggcat
    49801 caaggtgctg ccaggcgcgt aataactggt tgaggataaa ctgactggtg accttccctt
    49861 ccccgccttc ccacgggtgg aatttgcgcg tggcgagaat gtccgccgct ttgtctgcct
    49921 gacccgtgag atgccacaaa ttgagcaact ctgctgtcat gtcgtcgcgt ttcagcgcaa
    49981 tttccagatt attttccaga cgcgccagtc gtttctccgg tgtggctccg cttaacttat
    50041 caagcaaatc ccgttcgaaa agcagacgtg catcctgcgg cgcaagctga taagcattat
    50101 caagataacg cgcggccagc tcataatcgt gttgcttatt ccacgcatgg atcgctaacc
    50161 cgcgccagcc gtcggcaaac tccggcgaca tctctacgca acgttgccat aaggtaatgg
    50221 ctttgttgta gctacgcttg ttgtagtaga agcaggccag taaatgacgg gcaaaccagc
    50281 actcttcaat actctccagc gccgccactt cttccagcgt attcgggaaa cggacaaact
    50341 gcgggaagac atcaatggct tttgcgacca gttcgccgcg ttcggctttc ggcaataaac
    50401 tcgcctgcag gtaaagcggc agcgtgcgct ggtagtccag tgcgttcagc atctctgccg
    50461 ccagggtggg cattccccag ttaatcagtt gcccggcggt catcagagcg ttaacgtcgc
    50521 gtccctgaca cagaccgcgc cactgcgcga gggctgattc gctacgacca tcgaaccagt
    50581 tcagccacca cagagtggcg ttcagcggat aatcacgcag cagtttcgcg cgctgcaaac
    50641 gcgcgttgtc ctgacgacca cttaacacca gcagcagatt atgcaggcaa agcacttcct
    50701 gattggttgg gcaggcacga agactttgtt ggcaaaaatc cagaccagcg tcgaagttac
    50761 cattacgcgc cgccagtcgt gccaggccat aatagccacc ggctttgctg ttgccgctcc
    50821 agaccgcacg ccagaaatcc tcttcggctt gttgatattg tccctgacgt tcgtaagcac
    50881 tggcgcgaat caaactggcc tgtccgcact gcggattttt gttcagcgca tgtgcgcgtt
    50941 tcagcgcctg agtggcatac gccaccgctt gcgggaaatc tgcgcggtta tattccagca
    51001 tcgccagcgc caggttacag cggtaatcca gcggatccag caccacgccg cgcaggtagt
    51061 aatcgaacgg tgaacggctg gcgtgatgat attgctccag atgctgaccg ataaaccagg
    51121 cttcatctgt actggtaatg tcttgtgctg cgagtggcgc tttggcgacg tccggcaacg
    51181 gcaactcttg cggctgatgt tcctgataac tcaggacaat attgccgtcg gcatcggaga
    51241 gctcaatggt cagcctttca ggattgatac cgtgcaacac gccctggatg gcggtcgcag
    51301 gcatcagtgc cacggcatca tcaagtaacg cgttgcattt gccgatttcg cggatcgcca
    51361 ggcggtatcc gttcaacgga gagatggcat acagccccca ctcaatcccc cgctcactac
    51421 gctggagttt tatcaccgca tcgcgggagg cattttgcac catgcccaaa gaatgataag
    51481 gcaggaaata ctgctcaaaa cgcttctctt cgtaagcatc aagccaggta aaatcaggct
    51541 ggttatcggc aaaaataccg gtcatcagtt cgatatacgg gccgttattg tcggtcagac
    51601 tcttgtccca cgcctggccg aattcactgt gtccccagct ccactgtttt ttacctggcg
    51661 cgatatggtg gttggcaacg tgtagcaaac cgccatcttc atcgtgacac cacgcgccaa
    51721 caaaatcgta ctgtgatttt tcagccatat atgaggttgg aacgggcaca tttttatagc
    51781 gagaaatgtc cactccggcg gagtagtcca ctttgtagta agtgccggtg gcgatgggga
    51841 aagcggagac ggcccgtttg ccgtgatcaa acaccgcagt cacatccggt gggaaaacgc
    51901 tctgatgccc ttccccccct ttcactgccg ggttggccca ccacaagaaa tgacgcggcg
    51961 tggcgttacc gttatagacg cggctggcga tttccagcgc cgcccggtca gggcgcaggg
    52021 tgaaacctgt catcacctgt aaaccatgca tcggctccgt ttcgcctacc cacaccgtct
    52081 gtgcaccgtc ttcatgggct tcgagggtga aatcaacggg cataaaggtg gtcgggcgat
    52141 gatgttgcgg ccagttaaac tcaatcccac cggaaatcca cggccccagc agccccacca
    52201 gcgcaggttt aatgacttca ttgtgataaa caaaatcgcg ctgtttcact ttatcccacg
    52261 cgcgatgcac ccgaccgccc agttccggca ggatcatcac tttgatgtag tcgttttcca
    52321 gccacaccgc ctgccaggat ttcagagttt tctgctcgct cagcgtatcg gtcacgccgt
    52381 agggataaac cgcgccggac gatccctgat aaacgcgatt ttccaggaac atgggatgta
    52441 tatcctgcgg cccggtttca taggtcggga tctcaacgcg ctcttgccac acttttactg
    52501 gagtcatggt gccctcaata ttaacaagac atactgaatt aaaagatttg tggcagtgta
    52561 ttgaacaatc tggcaatgtt ttcgcggaat aatcacgcaa ttaactaaac aaggtttagt
    52621 gaagatgaga gcctgcatta acaatcaaca gattcgccac cataacaaat gcgtgattct
    52681 ggaactgctg taccggcaaa agcgcgccaa taaatcaacg ctggcccggc tggcgcaaat
    52741 ttcgattccg gcagtcagta atattttgca ggaactggaa agcgaaaaac gggtggtgaa
    52801 tattgacgat gaaagccaga cgcgcgggca tagtagcggt acatggctga ttgcgccgga
    52861 aggtgactgg acgctgtgcc tgaacgtaac gcccaccagt attgagtgtc aggttgctaa
    52921 tgcttgttta agtccgaaag gtgaatttga gtatttacag attgatgcac cgacgccgca
    52981 ggcgctgctg tccgaaatcg aaaaatgctg gcatcgtcat cgcaaactgt ggccgggccg
    53041 caccatcaat ctggcgctgg caatccacgg gcaggttgat ccggtgaccg gcgtatcgca
    53101 aaccatgccg caagcgccgt ggacaacgcc ggttgaggtg aagtatctgc tggaagagaa
    53161 gctgggcatt cgggtgatgg tcgataatga ctgcgtgatg ctggcgctgg cggagaaatg
    53221 gcaaaataat tcgcaggaac gggatttctg cgtgatcaac gttgattacg gcattggctc
    53281 gtcgttcgtg attaacgagc aaatttatcg cggcagtttg tatggtagcg gacagattgg
    53341 tcacaccatc gttaatccgg atggcgtcgt ctgcgactgt ggacgttacg gctgcctgga
    53401 aaccgtcgcc tcgttaagtg cattaaaaaa acaggcgcgg gtatggctaa aatcacaacc
    53461 ggttaatact cagcttgatc ctgaaaaact gacaaccgcg cagttaatcg ctgcctggca
    53521 gagtggagaa ccgtggatca ccagctgggt tgatcgctct gccaatgcca ttggtttgag
    53581 tctgtataac ttcctcaaca tcctcaatat taatcagatt tggttgtacg gtcgcagttg
    53641 tgcctttggt gagaactggc ttaatactat tattcgccag acaggattta acccgttcga
    53701 ccgcgacgaa ggaccgagcg tgaaagcgac gcaaattggc tttgggcaat taagccgcgc
    53761 acaacaggtg ctgggaattg gctatttgta tgttgaggcg cagttacgac agatttgatg
    53821 gcgcgataac gtagaaaggc ttcccgaagg aagccttttt tcaacagatt aaccgtttcg
    53881 cttatgcgta aaccgggtaa cgtgcgcaga tgtcgagaac tttacctttg atgcgctcga
    53941 taacggcttc atcattgatg ctgtccagca cgtcacacat ccagccagcc agttctttcg
    54001 cttctgcttc tttgaagccg cgacgcgtaa ttgccggagt acccacgcga ataccggagg
    54061 tcacaaacgg gctcttcgga tcgttcggta cgctgttttt gttgacggtg atgttagcac
    54121 ggcccagagc ggcgtctgct tctttaccgg tcaggttttt atcgaccaga tcaaccagga
    54181 acagatggtt atcagtaccg ccggaaacca ctttgtagcc gcgttccagg aacacttcta
    54241 ccatcgcttt agcgttttta gcgacctgct gctggtaagt tttgaactca ggctccatcg
    54301 cttctttcag agcaaccgct ttaccggcga ttacgtgcat caacggaccg ccctgaccac
    54361 cagggaaaac ggcagagttc agttttttgt acagctcttc gctaccacct ttcgccagga
    54421 tcaggccgcc gcgcggaccc gccagggttt tgtgagtggt ggtagtaaca acgtgagcat
    54481 gaggaaccgg gttcgggtag acgccagcag caaccaggcc cgcaacgtgc gccatatcaa
    54541 cgaacaggta agcaccgatg ctgtcagcga tttcacgcat tttcgcccag tccaccacgc
    54601 cggaatatgc agagaaacca ccgataatca ttttcggctt gtgttctttg gcttgttttt
    54661 ccagatcggc gtagtcgata tgaccggtag catcgatacc gtaaggaacg atgttgtaca
    54721 gtttaccgga gaagttaacc ggagaaccgt gagtcaggtg accgccatgc gccaggttca
    54781 tacccagaac ggtatcacct ggttccagca gcgcggtgta gaccgcaaag ttagcctggg
    54841 agccggagtg cggctggacg ttagcgtagt cagcgccgaa cagttctttc gcacgatcga
    54901 tcgccagttg ttcaacgata tcaacatact cgcaaccgcc gtagtagcgt ttgcccggat
    54961 aaccttcagc atatttgttg gtcagctgag aaccctgcgc ctgcattacg cgcgggctgg
    55021 tgtagttttc ggaggcgatc agttcgatgt gctcttcctg acgtactttt tcctgctcca
    55081 tagcctgcca cagttcggca tcataatcgg caatgttcat ttcacgcttt aacatccgca
    55141 tctcctgact cagctaacaa taaaattttt ggcctttata ggcggtcctg ttggacaacg
    55201 gcgaacagta taaccgaatc attgtgcgat aacaggtctt gacaaaggaa tttacgcaaa
    55261 cgattacctt caggctacgc aaggctttgg agaataaaga gcttgcaacc ggaaacggat
    55321 ttcttttcag gtttgtgatg caaatttttc acttcatcac attctttctg aaaaacacca
    55381 aagaaccatt tacattgcag ggctattttt tataagatgc atttgagata catcaattaa
    55441 gatgcaaaaa aaggaagacc atatgcttga cgctcaaacc atcgctacag taaaagccac
    55501 catcccttta ctggtggaaa cggggccaaa gttaaccgcc catttctacg accgtatgtt
    55561 tactcataac ccagaactca aagaaatttt taacatgagt aaccagcgta atggcgatca
    55621 acgtgaagcc ctgtttaacg ctattgccgc ctacgccagt aatattgaaa acctgcctgc
    55681 gctgctgcca gcggtagaaa aaatcgcgca gaagcacacc agcttccaga tcaaaccgga
    55741 acagtacaac atcgtcggtg aacacctgtt ggcaacgctg gacgaaatgt tcagcccggg
    55801 gcaggaagtg ctggacgcgt ggggtaaagc ctatggtgta ctggctaatg tatttatcaa
    55861 tcgcgaggcg gaaatctata acgaaaacgc cagcaaagcc ggtggttggg aaggtactcg
    55921 cgatttccgc attgtggcta aaacaccgcg cagcgcgctt atcaccagct tcgaactgga
    55981 gccggtcgac ggtggcgcag tggcagaata ccgtccgggg caatatctcg gcgtctggct
    56041 gaagccggaa ggtttcccgc atcaggaaat tcgtcagtac tctttgactc gcaaaccgga
    56101 tggcaaaggc tatcgtattg cggtgaaacg cgaagagggt gggcaggtat ccaactggtt
    56161 gcacaatcac gccaatgttg gcgatgtcgt gaaactggtt gctccggcag gtgatttctt
    56221 tatggctgtc gcagatgaca caccagtgac gttaatctct gccggtgttg gtcaaacgcc
    56281 aatgctggca atgctcgaca cgctggcaaa agcaggccac acagcacaag tgaactggtt
    56341 ccatgcggca gaaaatggcg atgttcacgc ctttgccgat gaagttaagg aactggggca
    56401 gtcactgccg cgctttaccg cgcacacctg gtatcgtcag ccgagcgaag ccgatcgcgc
    56461 taaaggtcag tttgatagcg aaggtctgat ggatttgagc aaactggaag gtgcgttcag
    56521 cgatccgaca atgcagttct atctctgcgg cccggttggc ttcatgcagt ttaccgcgaa
    56581 acagttagtg gatctgggcg tgaagcagga aaacattcat tacgaatgct ttggcccgca
    56641 taaggtgctg taatttgatg ttgccggatg gaaacatccg gcaacccttg acgcggttta
    56701 aattgccgcg tcgtcctctt caccggtacg gatgcgaatg acccgtgcca cgtcaaagac
    56761 gaagatttta ccgtcaccga ttttgccggt ttgcgccgtg cgaataatgg tatcgacaca
    56821 ggtatcgaca atgtcgtccg gtacgacaat ctcaattttc actttcggca gaaaatccac
    56881 catatactcc gcgccgcggt acagctcggt atggcctttc tggcgaccaa agcctttcac
    56941 ttcggtcacc gtcatgccgg taataccgac ttcggccagt gcttcgcgga catcgtccag
    57001 cttgaagggt tttataatcg catcaatctt tttcatgcta ttccttgaaa aggtcgcctg
    57061 tcttttgatc tgctaaacgt aacacataac gccaattcat tccttgaaat cgtttgcatc
    57121 cagctcgtgt cgggaaagca gtttataaaa ttctgtccgg ttgcgccccg ccattctcgc
    57181 cgcgtgggtg acgttgcctt tggtaatttg cagcagctta cgcaaatagt tgagttcaaa
    57241 ctgattacgt gcctcaacaa aggttggcag cgccgtgttt tcaccctcca gcgcctgctc
    57301 tactaatgca tcgctaataa ccggagatga ggtcagcgcc acgcactgtt caatcacgtt
    57361 gaccaactgg cgcacattac ccggccagct cgcggtcatc aggcgtttca tcgcatcagt
    57421 ggagaacgcg cggacaaacg gtttatgtcg ctctgccgcc tggcgcaaca ggtgatttgc
    57481 cagaagcgga atgtcttctg tgcgctctgc cagagccgga attttcaggc tgacaacgtt
    57541 gaggcggtaa tagagatctt cacggaattc cccgcgcgcc atcgcttttg gcaggtcgcg
    57601 gtgagtggcg gaaataatcc gcacgtcgat atcaatatcg cggttgctgc ccagcgggcg
    57661 cactttacgc tcctgcaaca cgcgcagcag tttgacctgc aatggtgcgg gcatatcgcc
    57721 aatctcatcg agaaatagcg taccgccttc cgccgcctgg aataaacctt cgcgattgct
    57781 gacagcgcca gtaaacgcgc cacgcgcatg accaaacagc tccgactcca gcaactgctc
    57841 gggcaatgcg ccacagttaa tagcaataaa tggtttgctg ttgcgcgggc tggcgttgtg
    57901 gatagcctgg gcgaaaatct ctttcccggt gccgctctgg ccattaatca acacgctgac
    57961 gtctgattgc gccaccagcc gcgcctgttc cagcaaacgc agcatcagcg ggctgcgggt
    58021 gacaatcgcc tcgcgccagc gttcgtcggt ggctggcgcg gattgttcca gcgcatcgtc
    58081 aattgcctga tatagcgcat ctttatcgac aggcttggtg aggaaactga acacgccctg
    58141 ctgtgttgca gcaacggcat cgggaataga accatgcgcg gtaagaataa ttactggcat
    58201 tcccggctgc actttctgaa tttcagcaaa cagctgcata ccgtccattt catccatccg
    58261 caggtcgctg atgactaaat ctactttttc gcgattcagt acccgtaatc cttcagcgcc
    58321 actttccgcc gtgaccacgc tgtagccttc gctggtcagg cgcaggccaa gcagtttcag
    58381 caatcccgga tcgtcatcga ccaataataa atgcgcaggt ttatggctca tcaggagtga
    58441 cctcatgggt ggatggcgcg ggcttttcac cctcgcgcgg cgtatccggc gagaaatttc
    58501 cagccggttt gcgggtcgag agctggcgtt caatatcagt caggttttcc agcttgcggg
    58561 tggtgagttc cagttgctgc tgtagaacgt gatgttgctg gcgcaatgta tccagctcgc
    58621 tgtcgctggc ctgctggagc ttgccgtagc gttggcgctc ttccgccagt tgcagttgca
    58681 gcgcctgacc gtcgcgccag agttgataca gagggcgaac ctgtgccggg atctcggtac
    58741 ttagcgcatc aatgcgcgcg accagctggc ggcgctcata cggcgtaatt ttggcgtcgg
    58801 cgagcaaaat cccttgttta aaggtatttt gccagctgcc gtcgtcatat tggcgagctt
    58861 gctgacgtga ctgcgcaggc attaaacgat cagcacaatc catcgcccgc agccagtaaa
    58921 gcggattggt ttcggttgat ttaccttgca gcgcccagat gtcgctacat tcagtagaaa
    58981 gatagtcagc cagttgataa accggaattt tttcttctgc tagcgtatca atggctggct
    59041 tattgtgact ctgcacgcaa cccagcaatg ccagacatgg cagccctgcc agccacagcc
    59101 gtcggggcaa taatcgttga aaaatgtgtc gcatattcac cagacttaaa gcctatccca
    59161 gtgggcgtaa ttgttgcaga cagtctggac atggacagcg cggagaaacc ggagcgtaca
    59221 tatcgtacgt gaagatttcg agcactgccc ggggccgaac tgacaaataa aatagcctat
    59281 aggataggct gttagcatta tttcgtgttt ttcgacgacg gtaattcaat gcggaaacaa
    59341 acgtcttgcc cgctctcgtc gaccagatac agttcccctt gcatacggcg aatacaatcc
    59401 ctggcaatgc ttaatcccag accgctgccc ttcaccgccc cttttcgctg gtggcttccc
    59461 tgaaaaaagg gttcgaagat catggcgcgt tcctcttgcg gaatgggcgt gcctgtattg
    59521 atgacatcaa tataaacccg cgcaccatgt gaactgctgc gaaggcaaat gttaccggat
    59581 tcagccccgt agtgcaccgc attggagtaa agattatcca gtacgctcat cagcagcatt
    59641 ggctccgcca ggcaagctgt tgctttgaga tcgacgtcgg tatgcatcat tttagcccgt
    59701 gcgggcaggc tatgagcaga aaccactgtc tccaccagcg gtgctaattc aacattctcc
    59761 agttccaccg cactgtccgc ctgtttacgg ttgtaatcaa gcagttgttc gatcagtttt
    59821 tgcaaattgc ggctgctgct atcaagaatg ctcaccacct ctttttgctc tggcgtaagc
    59881 ggcccgacaa cctggtcagc cagtaattca gtgccctcgc gcatactcgc cagtggcgtt
    59941 tttaattcat gagataaatg tcttaaaaat tgatggcgtt gggattccag ccatgacagg
    60001 cgctcactta accaaagaat acgttgccca accgagcgta actcgctcgg tccactgaac
    60061 gagacgctat tgcccagaga acgcccttcc cccagacggt tgatcatgcg ctcgatattt
    60121 ttcaccggac caataatcat ccgcgtgaaa agcagtacca ttaccagact caccagaaat
    60181 agcaccagcg attgccaacc aaaatattgc ccacgttcgg cgatttcacg ctgaagttgc
    60241 tgcccacgag agaacaccac tgtgcgcgtg gcctgcacca tttcggtatt ggcactggca
    60301 aaggcttcaa gacgcgcggc ggcggcagca tcgggaccac tgttgttaca ctgaagttgg
    60361 gccagattgt tcaaatcctg acgcaatgcc tggtaaagtt tatcgtctgg cagtacgcct
    60421 gcgtgggcat cgagcatttc gctgtaacgc ttgcgctggc tttgataaac cttcgcaagc
    60481 gtagggtcgt ccagcacgca atactgacgg tagctacgct ccatctccag cgccgcgttg
    60541 gtcattgctt cgctgcgccg ggcatcgata agcgtggtgc ggttgaccag cgccgcctgg
    60601 tcgctaagcg cattcaggct ttgccaggct tgccatgcca gcaccaacaa gggcagcaga
    60661 atcagcaaaa atgccagcat taccagttgt cgtaatgagc ggggaaaaac gggccagcgt
    60721 ttcaaggtgt tactctcgtc agacgcgaat agcctgatgc taaccgaggg gaagttcaga
    60781 tacaacaaag ccgggaatta cccggctttg ttatggaata aggcggtgcc taactcgacg
    60841 tttcgcccga tggttgatat agctacgctg atatcagaag ttggacggca ggcaccttgt
    60901 tgtgcgtcat tcgtatttta tgtagcacgt cccgaagggg ctgacataag tcggtgaatg
    60961 agccactgct tactattatg cagtaactgt gccaataaag aaaatagttt ggtaacgtac
    61021 tgattatacg ctgctttgag gggtttatgg cttctccgct gtttgaatgt aaatcagcca
    61081 ctgtgtcgct aaaaagagac aacttaagat aaacttatta gattacatta aaatcaatca
    61141 tttaagtgtc gccagaaagc gacacggtaa accacccatt gtcgtgattt acagacacaa
    61201 aaaagctccc tgagttggga gcttataata gcggttggta tttaatgccg gatgcggcgt
    61261 gaatgccgca tccgggctgc aaaaccaatg ggctgacgac ttaccccaac tgcttacgcg
    61321 cattgcggaa aatacgcatc catgggccat cctcgcccca gttttccgga tgccaggagt
    61381 tgctgacagt acggaaaaca cgttccgggt gcggcatcat aatggtgact cgaccacttt
    61441 cagtcgtgac tgccgtaata ccgttcggag aaccgttcgg gttagccggg taggtttcag
    61501 tgactttgcc gaagttatcg acatagcgca gtgccaccag ccctttgctt tccagtgccg
    61561 ccagatgcgc cgcatcacgc acttccacgc gcccttcacc gtgagagaca gcaatcggca
    61621 tttgcgagcc caccatcccc tgcaacagca gcgacgggct ttgggtcact tcaaccaggc
    61681 tgaaacgcgc ttcaaagcga tcggaggtat tgcgcacaaa acgtggccac aactcactac
    61741 ctgggatcag ttcacgcaga ttagacatca tctggcaacc gttacatacc cccagcgcca
    61801 gcgtttgcgg acggtggaag aaggttgcaa actcatcgcg tacacggtca ttgaacagga
    61861 ttgacttcgc ccaaccttca ccggcaccca gcacatcacc gtaggagaaa ccaccgcacg
    61921 cgaccagggc gtggaaatct tccaggcccg tgcgtccagc cagcaggtca ctcatatgca
    61981 cgtcgatagc atcaaagcct gcacggtgga aagctgccgc catttcaaca tgcgagttca
    62041 cgccctgctc acgcagtacg gcaactttcg ggcgcgcgcc agtggcaata aacggtgctg
    62101 ccacatcttc gttgatatcg aacgacagtt ttacattcag gcccggatcg gcgtcgtttg
    62161 atttcgcctg gtgctcctgg tcagcacact ccgggttgtc acgcaggcgc tgcatctgcc
    62221 aggtagtttc tgcccaccag acacgcaacg tagtgcggct ttcgctgaat acagtctgcc
    62281 cgttggcggt aatcacaaaa cggtcaccgg aaaccgcctg cccaacataa tggacgcaat
    62341 cagcaagccc atgctgtgcc agtacggact cgaccgcttc acggtcagcg gcacgaacct
    62401 gaatcaccgc acccagttct tcgttaaaca acgccgccag gcgatcgtca cccagagagc
    62461 cgatatccgc atcaatgcca caatgaccag caaaggccat ttccgccagt gtgaccaaca
    62521 gaccgccatc agagcggtcg tgatacgcca gcagcttacg ctgtgcaacc agcgcctgaa
    62581 tcgcgtcata gaagcctttc agttgcgcga catcgcgtac atctgccggt ttgtcgccaa
    62641 gctgacgata aacctgcgcc agcgccgttg cgcccagcgc gttattgcct ttgcccaaat
    62701 caatcagcag cagtgcgtta tcttcggtag aaagctgcgg cgtgatggtg tgacgtacat
    62761 cttccacgcg ggcaaatgca gaaatcacca gcgacagcgg cgacgtcatt tcgcgctctt
    62821 cgttaccttc ctgccagcgg gttttcatcg acatagagtc tttacccacc gggatcgtca
    62881 ggcccagcgc cggacaaagc tcttcgccca cggctttaac ggcttcatac aggcccgcat
    62941 cttcaccagg gtggcctgcc gccgccatcc agttggcgga aagtttaatg cgtttgatat
    63001 cgccaatctg tgttgcggca atattggtta acgcttcacc gaccgccaga cgggcagagg
    63061 cggcgaaatc cagcagcgca accggcgcac gctcgccaat cgccatcgct tcaccgtagt
    63121 agctgtcgag gctggcggta gtgaccgcgc agttagcgac cggcacctgc cacggaccca
    63181 ccatctgatc gcgcgctacc atgccagtta cgctgcggtc accgatggtc accaggaaag
    63241 ttttctccgc cacggtcggc agatgcagta cgcgtttcac cgcgtctgca atggtgatcc
    63301 cttcacggac cagcgcatcg cccttcgctt tcagcgtttg tacatcgcgg gtcatcttcg
    63361 gcgttttacc aagcaggacg tccagcggca gatcgatcgg ctgattatca aaatgacgat
    63421 cgtgcagaga aagatgcagt tcttcggtcg cttcaccaat caccgcgtag ggtgcgcgct
    63481 cacgcttaca cagttcgtca aacagcggta attgatcggc agcaaccgcc agcacgtagc
    63541 gttcctggga ttcgttacac cagatttcca gcgggctcat gcctggctcg tcgctcagaa
    63601 tgtcgcgcag ttcaaattta ccgccacgcc cgccgtcgct caccagttcc ggcatagcgt
    63661 tagaaagacc gcctgcgcca acgtcgtgga taaacagtat tgggttggca tcaccaagct
    63721 gccagcaacg gtcaatcact tcctggcagc gacgctccat ctccgggttg tcgcgctgta
    63781 cggaagcaaa gtcgaggtcg gcatcagact gaccagacgc catggaagac gccgcgccgc
    63841 cgccaagacc gatgttcatt gccgggccgc cgagaacgac cagcttcgcg ccaacgttga
    63901 tctcgccttt ttgtacgtga tcggcgcgaa tgttgccgat cccgcccgcc agcatgatcg
    63961 gtttgtgata accgcgcagc tcttcgccgt tgtggctgtt cactttttct tcataagtac
    64021 ggaagtagcc gttcagtgcc ggacgaccaa attcgttgtt aaacgccgca ccgcccagcg
    64081 ggccttcggt catgatgtcc agcgcggtga caatgcgctc aggcttaccg aaatcttctt
    64141 cccacggctg ttcgaagcca ggaattcgca ggttggaaac ggagaaacca accaaacccg
    64201 ctttcggctt cgcgccgcgc ccggtggcac cttcatcgcg gatttcaccg ccggaaccgg
    64261 tcgccgcgcc cggccacgga gaaatcgccg tcgggtggtt gtgagtttcg actttcatca
    64321 gaatatgcgc cggttcctga tggaaatcat agcggcccgt ttcgtggtca gcaaagtagc
    64381 ggcccacttc agaaccttcc attacggcgg cgttatcttt ataagcagag agaacgtgat
    64441 ctggcgtggt ttcgaaagta tttttgatca tcctgaacag cgatttcggc tgttgttcac
    64501 cgtcgataat ccagtcggcg ttaaaaattt tgtggcggca gtgctcagag ttcgcctgag
    64561 cgaacatata cagctcgatg tcgttcgggt tacgaccaag ctttgtgaaa gcatcctgca
    64621 gatagtcaat ttcatcttcc gccagagcca ggccaagacg caggttagcg tcgatcagcg
    64681 cctgacggcc ctgccccagc aaatcaacgc ttgtgaccgg agtcggttga tggtgggcaa
    64741 acaactgctc tgcatcatct aaatcaaaaa agaccgtttc catcatgcgg tcgtgcagtt
    64801 cagcggtaac ctgctgccat tgttcattgg tcagcgtacc ggcttctata tagtaagcaa
    64861 cgccgcgctc aaggcggttt acctgttgta gcccgcagtt atgggcaata tcggtcgctt
    64921 tcgaagacca gggagagatg gtgccaggac gcggggtcac cagcaggagt ttgccttgcg
    64981 gggcgtggct ggcgagtgcc ggaccatatt tcagcaggcg ttcaagttgt gcgtgctcat
    65041 catcgtttaa cggcgcattg aggtcagcaa aatggacata ctcggcgtaa atattgtgaa
    65101 ccgggagcct ggcagcctga aaacgtgcca gcagtttgtt gattcggaat gccgacagtg
    65161 caggcgaacc acgcagaatt tccatcataa gtctctcgtc ttctaagctt tcggtgtacc
    65221 caaggggggg aaacgggcgt cattataaag aatctgatgc gctgacgaaa ccgtttgcgt
    65281 ggaaataaaa tcaccatcgt gaattagcaa cgcgtgccgc caatggctgt aataagttgc
    65341 catctggcgc aggtttacgc aaaatgccgc tcatttatga gtaaaccttt cactattatt
    65401 acgttttttc aagctgggac gcgcacgaca cagagaatta actaattgaa aaaattaaag
    65461 attaattatc tgttcatcgg cattctggca ctgctgctcg tggtcgctct ctggccatcc
    65521 attccctggt ttggtaaagc cgacaaccgt atcgccgcca ttcaagcgcg gggacagttg
    65581 cgtgtaagca ccattcatac tcccctgact tataacgaaa tcaacgggaa accttttggc
    65641 ctggattacg aactggcgaa acagtttgcc gattacctcg gtgtaaaact gaaagtgacc
    65701 gtgcggcaga atatcagcca gctgtttgac gacctcgata atggtaacgc cgacctgctg
    65761 gcggcaggac ttgtctataa cagtgagcgg gtaaaaaatt atcagcctgg ccctacctat
    65821 tattccgtgt cacaacaact ggtttataaa gtgggtcagt atcgcccacg tacgctgggc
    65881 aacctgacgg cggagcaact caccgttgca ccgggtcatg tggtggttaa cgatctccag
    65941 accctgaaag aaacaaaatt cccggaatta agctggaagg tagacgacaa aaaaggctct
    66001 gcggaattaa tggaagatgt catcgaagga aaactcgatt acaccattgc tgattctgtc
    66061 gccatcagcc tgtttcagcg cgttcacccg gagctcgccg tagcgctcga tatcaccgat
    66121 gaacaaccgg tgacttggtt tagcccgtta gatggcgata ataccctttc cgccgccctg
    66181 ctcgacttct tcaacgaaat gaatgaagac ggtacgctgg cacgcattga agagaaatac
    66241 ctggggcatg gcgatgattt tgattacgtc gatacgcgca catttttacg cgccgtcgat
    66301 gcggtactgc cgcagttaaa gcccctgttt gagaaatacg ccgaagaaat tgactggcgt
    66361 ttgctggccg ctattgctta tcaggaatcg cactgggatg cacaggccac ttcaccgacg
    66421 ggtgtgcgcg gcatgatgat gttaaccaaa aataccgcgc aaagcctcgg cattacggat
    66481 cgtaccgatg ccgaacagag catcagcggt ggcgtgcgtt atttgcagga tatgatgagt
    66541 aaagtgccgg aaagtgtgcc ggagaacgag cggatctggt ttgccctcgc tgcgtacaat
    66601 atgggctatg cgcatatgct ggatgcccgc gccctgacgg caaaaaccaa agggaatcct
    66661 gacagttggg ctgacgtaaa acagcgtctg cctttactta gccagaaacc ctattacagc
    66721 aagctgactt acggctacgc tcgtggacat gaagcctacg cttatgtcga aaatattcgt
    66781 aagtatcaga ttagcctggt gggttatctg caagagaaag agaagcaggc tacagaagcg
    66841 gcgatgcaac tggcgcagga ttatccggcg gtatcgccta cggagttggg caaagagaaa
    66901 tttccttttc tctcgtttct ttcccagtcg tcatcaaact atttgaccca ttctccctct
    66961 ctgctgtttt ccaggaaagg gagtgaagag aaacaaaatt aatccgtcga ggattgcgct
    67021 tttttctgcg ctttaatttc ctggcggcgc atgcgaaaga agtcactgag caacgccgcg
    67081 cactcatccg ccagtattcc ttccgtaatt tccactcggt gattcatacc cggatgatgc
    67141 agcacatcca ttaaagatcc cgcagcgcca gttttcgcgt cacgcgcacc aaagaccacg
    67201 cgaccaatgc gactgtggat catcgctccg gcacacatta cacatggttc aagcgtgaca
    67261 tacaacgtgg cgtcgatcag acgataattt tgcatcacca gaccaccctg ccgcagggcc
    67321 atgatttctg catgtgcggt gggatcatgg cgaccaatcg ggcggttcca gccttcgccg
    67381 attacccgat tgttatgcac taataccgcg ccgaccggca cttcccgctc atcccaggca
    67441 cgtttcgcca gcgtcagcgc gtgacgcatc cagtattcgt ggctaaattc gacttcagac
    67501 aaaaagaaaa ctccggttat aaaagcgcgg cgcattatac acggacgcta tgctttactc
    67561 tattccagtt gctggagttc accgcgcggg gttactcgcc aacgatgctg acagaaataa
    67621 agcagcggat tgtcctgatt actgtcgcta tagccactgt acagccgcag cggagtgccg
    67681 attttgcgct ccagttgtgc gaccttttca tgtcccagac aacgcatcgt caatacccaa
    67741 ccaccatagc cacgctgaat ttggctggcg ataagattaa cccgcggcag ccagggcgta
    67801 tcgaaataaa ccgcttcaac cagcggctgc ggagagccgg taatcaacca gatatcagca
    67861 tcggaactta acaggtaggt ggttaatcgc tcctgaacca gcggaaaggc ggtaacattg
    67921 tcgcgaaacc agcgcacgaa atcggcctgc aacgtctgta aacgtgcttc gctgtgacca
    67981 aaagtgcccc cccacagaag cagactcatc ggccagcgtg ccgcacgacc ttttatcaat
    68041 aacgcaatgg ctataatcgg taacaacggc aggacaagta acgcattcag cggttggcga
    68101 cgcagtaaat agcgcagaaa actgccgaac atatcctgct gatgcaatgt tccatctaag
    68161 tcaaaaaaca ccacacgacg ctcgtgagtt gccaaaccat actcctctgg actgaaacat
    68221 cctgattaat tttcgctact acatagccta acagatagat catcactttt ccggcaacaa
    68281 tcgaccgcat catggctaac tggaattttt aattcatggc aattagcggc aatggaatat
    68341 aaaattcact cgcgtgtctc tcatattgcc tgttgtcgcc actatttcat cgcgaggaaa
    68401 gatatgaacg gcttacttcg tatccgtcag cgttaccagg ggcttgccca gagtgataaa
    68461 aaactggcgg attatctgct gctacaacct gatacggcgc gccatttaag ctctcagcaa
    68521 ctggccaacg aagccggagt cagtcagtcc agcgtcgtga agttcgcgca aaaactcggc
    68581 gataaaggtt ttccggcgct taagttggcg ttgagtgaag cgctggcaag ccagccggaa
    68641 tcgccctccg tgcccattca taaccaaatc cgcggtgatg atccgttacg gctggtcggc
    68701 gaaaaactga ttaaagaaaa tactgccgct atgtacgcaa cgctaaacgt taatagtgaa
    68761 gagaaactgc atgaatgcgt aacaatgttg cgctctgcgc ggcggataat tctgaccggt
    68821 attggcgctt cgggtctggt ggcgcaaaac tttgcctgga agctgatgaa gattggcttc
    68881 aatgctgccg cagtgcgcga tatgcatgcg ctactcgcaa cagtacaggc gtcgtcccct
    68941 gacgatctgt tattagccat ttcctacacc ggtgtacgac gcgagttaaa cctggcggca
    69001 gatgagatgc tgcgagtggg cggaaaagtg ctggcgatta ccggctttac tccgaatgcc
    69061 ctgcaacagc gtgcttctca ttgcctgtat accattgccg aagaacaagc gacaaacagt
    69121 gcttcaatct ctgcttgtca cgctcaggga atgttaacgg atttgctgtt cattgcgctg
    69181 attcagcagg atctggaact ggcaccagaa cgtattcgtc atagtgaagc gctggtgaaa
    69241 aaactggtct gagtaaagaa tgcgcgtata atgcccgccc ggtttgtgtt gttttgagag
    69301 tttccttatg gcgttgttaa tcactaaaaa atgcatcaat tgtgatatgt gtgaacccga
    69361 atgcccgaat gaggcgattt caatgggaga tcatatctac gagattaaca gcgataagtg
    69421 taccgaatgc gtagggcact acgagacacc aacctgccag aaggtgtgcc cgatccccaa
    69481 tactattgtg aaagatccgg cgcatgtcga gacagaagaa cagttgtggg ataaatttgt
    69541 gctgatgcac cacgcggata aaatttaata ttctactctg gaagtagagt attaattata
    69601 ttactgggaa gccttaacgc cattatattt atttaattga tgacattagc ataatcattc
    69661 actaagttaa tttatatagt atctgccaag acacttattt atagttatta aaggcgcgtc
    69721 cgattggttc accggacgca ccttaagtac gtttccttgt gttataagaa cagaaggatc
    69781 agctgtaaaa cagcaatgat gattttgatg acccgtttaa tcaggtatcg gcaatcagtc
    69841 attcgttttt ccttaaacaa ggaatgcagc tgttcagatt agcccttaca tctccccaaa
    69901 actgaacgtg cgagttattg agggtgcatg ctgcactcca caccagagct ttgacgacac
    69961 cactcgtttc aatgggggaa ttctgtggca tggtgtaaag cacagcaaaa tcttcaataa
    70021 cgaagccaat tttaatgtac ttacgaattg gcggtcacgt taatctttcc atcaatattg
    70081 ctttcttcgt aaaggctcga gtttttatgc taaagattgc aagttgcttg taaaagataa
    70141 gtacactgat ccataatcgc tgttgttgag ggtgcatgct gcacaaaatt aaagttaaaa
    70201 agtaaaaccc ccgttcctta ccagttcggg ggttttactt tttaaagaga acggtattat
    70261 tttaactttc aataattacc gtggcacaag catagtggcg ttcatctgcc agcgttacat
    70321 gcatatttac aacgccgagc ttttccgcca gttttaatgc ctcaccccat agccgtagcc
    70381 gtggtttgcc gagctcatca ttgaatactt caaattgatt aaacgccaga ccattgcgga
    70441 tcccggtgcc aaacgctttt gctgcggctt ctttcacagc aaaacgcttc gccagaaaac
    70501 gcaccggctg gtggtgcgtt ttccagattg cccattcgtt atcgcttaat acgcggcgtg
    70561 ccaggcgctc accggatcgg gcgatcactg cttctatgcg agcgatctct acgatatccg
    70621 tgcctaaacc taatattgcc attagccacg cgcttccagc atcagacgct tcatttctgc
    70681 caccgcatct ttcagtccgg tcatcactgc acgaccaata atggcatgac cgatattcag
    70741 ttcatgcatc tcagggatgg cggcaatagc tttcacgttg tgataggtca gaccgtgacc
    70801 agcgttaact ttcagaccga gactagcggc aaaggtcgcg gctttggcga tacgcgccag
    70861 ctcttgcgcc tgttcggcgt cagttttggc atcagcatag caaccggtgt ggatctcgat
    70921 aaacggtgcg ccaacctctg ccgcagcttt gatctgctct tcatcggcgt cgataaagag
    70981 agaaacctgg atcccggcat ctgccagacg tttgcaggca tcgcgcattt tgtcacgctg
    71041 ccctgcgacg tccaggccgc cttcggttgt tacttcctga cgcttttccg gtaccagaca
    71101 gcaaaaatgt ggcttcgtct caacggcgat cgccagcatc tcttcggtca ccgccatctc
    71161 cagattcatg cgggtatcca gcgtctgacg caggatgcgc acatcacggt cagtaatgtg
    71221 gcggcgatct tcacgtaaat gcacggtaat gccgtccgct cccgcctgct cagcaataaa
    71281 cgccgcctgc accggatccg ggtaagcagt accgcgtgcg ttacgcagcg ttgcaatatg
    71341 gtcaatgttg acgcctaaca gtaattcagc catgacaatc ctcatcattc ataatgtgtt
    71401 ttcaccgttc gcttaggcat aaactgccgg aacagttccc tgctctttaa tggtttaccg
    71461 ccaagatacg gcttaagcgc catgcgggta aagcgtttcg cggcgcgtag tgtgtctgcg
    71521 tcaggaaatt cccgcgcgtt taacgctttt aactgccttc cggtgaacgt tttattgtcg
    71581 ataacgacgc ttgcgataaa ccctttttct tcgcgataac gatacgtcat ggtgtcatct
    71641 accggctcgc cgctacccgc acaatgggta aaattgacgc catagcccag atgcccgagc
    71701 agtgccaatt caaagcggcg cagcgcgggt tctggcgtac cagtggcacc tgcaagagac
    71761 tgaatgcagt gcaagtaatc gaaaaagagt tcagagaagc gcgtctcgta ttccagtacg
    71821 cgggagagaa gttcgttgat gtacagaccg ctgtaaagcg tgataccgct taatggcagc
    71881 gccagcgaga cagcttcagc actgcgcagc gttttgactt cgccacgccc gccgaaacgt
    71941 agcaagagag gggtgaaagg ctgtaatgca cctttcagag tagagcgttt agagcgtgcg
    72001 cctttggcaa ccagacgcac gcgacccgat tcctccgtga agacgtccag catcaggctg
    72061 gtttcgctcc acgggcgact atgcaggaca aatgcgcgct gccagccttc catcggagtg
    72121 actcttaaag atcgtcaacg taaccgagac tgcgcagtgc gcgttcgtcg tcggcccaac
    72181 cggatttcac ttttacccac agttcaaggt ggacaggcgc ttcgaacatt tcctgcatgt
    72241 ctttacgcgc ttcaatcccg atggttttga tcttggcccc tttgttgcca ataaccatct
    72301 tcttctgccc ttcacgctca acgagaatca aaccattgat gtcataacca ccgcgttcgt
    72361 tagagacgaa acgttcgatc tccacggtca cggagtacgg cagttcagcg ccgaggaaac
    72421 gcatcagttt ttcgcggatg atttcagacg ccataaaacg ctgtgagcga tcggtgatgt
    72481 aatcttccgg gaagtgatgg gtcgcttcag ggaggtgctt acgcacgatt gctgcgatag
    72541 tgtcgacatt cagcccggtt tcagcagaga tcggcacgat atcgaggaag ttcatctgac
    72601 ttgccaggaa ctgtaagtgc ggcagcagat cggctttttc ctgcacgtta tccactttgt
    72661 tcaccgcgag gatcaccggc gctttgccat cgcgcagttt gttgagcacc atttcgtcgt
    72721 ccggcgtcca gcgggtgcct tcaacgacaa aaatcaccag ctcaacgtcg ccaatagagc
    72781 tgctcgccgc tttgttcatc aggcggttaa tagcgcgttt ttcttccata tgcaggccag
    72841 gggtatcaac gtagatcgcc tgatacgcgc cttcagtatg gatccccaca atgcggtgac
    72901 gagttgtttg cgctttgcgg gaggtgatgg agatcttctg ccccagcagt ttgttcaaca
    72961 atgtggattt gccaacgttc ggacgtccga cgatggcaat aaatccgcag taacttttat
    73021 cgatgctcat tccagctcca gttttttcaa cgcctgttcg gcggcagcct gctcagcctt
    73081 acgacggctt gaacctgtgc caaccaccgg ttcactcagg ccgctgacct ggcagtggat
    73141 agtaaattcc tgatcgtgcg cttcgccacg tacctggact accagataag tcggcagcgg
    73201 cagatggcga ccctgcaaat attcttgcaa gcgcgttttc ggatcttttt gtttatcgcc
    73261 tgggctaatt tcgtccaaac gagtttgata ccagttgagg attaatttct cgacggtttg
    73321 aatatcactg tcgaggaata cgccaccaat taatgcttcg acggtgtcgg cgagaattga
    73381 ctcacgacga aatccaccgc ttttaagttc acctggccct aaacgtaagc actcgcctaa
    73441 ctcaaattcg cgcgccagtt ccgccagcgt attgccacgg accagcgtgg cgcgcatccg
    73501 gctcatatcg ccttcatcca cacgagggaa acggtgataa agcgcattgg cgataacgta
    73561 gctcagaata gagtcgccta aaaattctaa acgctcgtta tgtttactgc tggcactacg
    73621 atgagttaat gcctgctgca acagttcctg atgattaaaa gtgtagccca gcttccgttg
    73681 aagccgatta attacgatgg ggttcatgcg ataccaataa ataaatgcgt caacaattca
    73741 gcacacgaaa cagacctgat atacatggct ctgctaactg cttcgctgca gtttcagttt
    73801 accggtatat ggggaccaac gctgtttcgt gtgccgtggc aacctggagg tgccaacctt
    73861 aaacttcggg ggaatattct atacacaacg acgggggatg tcgttagcca cgggagattt
    73921 atctcataaa taattcacgt tgtcgccata acggcgacaa cgtgaacgaa gatgagctat
    73981 taatggatgc cgccaatgcg acttaagcgc acaccagtcg gccattcgcc ttcttgctta
    74041 tcgaagctca tccagatagc cgttgcccga ccgaccagat tcgcttcagg cacaaacccc
    74101 caataacggc tgtccgcgct gttgtcgcgg ttgtcgccca tcatgaagta ttgtcccgga
    74161 ggaacaatcc aggttgccag ttgttgccct ggctgctggt aatacatccc cacctgatcc
    74221 tgcgcaatcg gcactgtcag aattcggtgc gtcacatcac ccaatgtctc tttacgctcg
    74281 gaaagacgaa ttccattttc tttggtttcg tttttcggca cttcaaagaa tccgctggtc
    74341 gcttccccac cattacggcg tgagaaggtc tgaacgaaat cgctcggttc cacgtttgag
    74401 taggtgaccg gcagcgcgtt ttcacacgcc tggccggaac tgcatcccgg ttgaatcgtc
    74461 agctctttcg agaccggatc gtaagtgact ttatcgcccg gtaaacccac cgcgcgcttg
    74521 atgtaatcaa gctttggatc ttccggatat ttaaagacca cgatatctcc gcgtttcgga
    74581 tgaccggttt cgatcagcgt tttctggtag ataggatctt taatgccata agcaaacttt
    74641 tctaccagaa taaaatcacc gattaacaga gtcggcatca tcgaacctga cgggatctgg
    74701 aacggttcat aaataaacga acgcacaatc aatacgatag ccagtaccgg aaaaacagaa
    74761 gcaccggttt ccagccagcc aggcttcggc gcaacctttt tcaacgttgc tttatccagt
    74821 gagtcaccgg cagccgcctg cgccgctgcc tgacgttccc gccgtttagg tgcgaaaaag
    74881 aatttatcca cgcaccataa aatgcccgtc accagtgtgg caatcaccag aatcagggca
    74941 aacatattcg ccatgccaac tcctaagggt tatttgttgt ctttgccgac gtgcagaatg
    75001 gcgaggaacg cttcctgcgg cagctcgacg ttaccgatct gcttcatgcg tttcttacct
    75061 tctttctgct tctgcagcag ctttttctta cggctgatat cgccgccata acatttagcc
    75121 agtacgtttt tacgcagctg tttcacggtg gatcgcgcaa taatgtgcgt accaatcgct
    75181 gcctgaatgg cgatatcaaa ctgctggcgt gggatcagat ctttcatctt ctccaccaac
    75241 tcgcgaccgc ggttttgcga attatcacgg tgggtgatca gcgccaacgc atcaacacgt
    75301 tcaccgttga ttaatacgtc tacacgtacc atgtcggacg cctggaagcg tttgaagttg
    75361 taatccagag acgcataacc acgcgaggta gatttcaggc gatcgaagaa gtcgagcacc
    75421 acttccgcca tcgggatctc gtacgtcagc gccacctgat taccgtggta aaccatattg
    75481 gtctgcacgc cgcgtttttc tacgcacagc gtaataacgt tgccgagata tgcctgcggc
    75541 agcagcatgt gacattcggc aatcggctcg cgcagttcgt agatgttatt taccgctggc
    75601 agcttggatg gactgtcgac gtaaataact tctcttgacg tggtttcaac ttcatacact
    75661 acggtcggcg cagtggtgat cagatccaga tcgtattcac gttccagacg ttcctggatg
    75721 atctccatgt gcagcaggcc gaggaagccg cagcggaaac caaagcccag cgcgctggag
    75781 ctttccggct catagaacag tgaagcatcg ttcaggctga gtttacccag cgcgtcacgg
    75841 aaggcttcat agtcgtcgga acttaccggg aacagaccgg cgtatacctg cggtttgact
    75901 ttcttaaagc caggcagcgc cttttctgcc ggattacgcg ccagcgttaa ggtatcgccg
    75961 actggagcgc cgtggatatc tttaatcgca cataccagcc agcctacttc gccacatttc
    76021 agttcagtgc ggtcaacctg tttcggcgtg aagatgccca gacggtcggc gttataggtc
    76081 tgcccggtac tcatgacttt cactttgtcg cccttacgca gggtgccgtt tttaatacgg
    76141 ataagtgaaa caacgcccag gtagttgtcg aaccatgagt cgataattag tgcctgcaac
    76201 gggccttccg gatcgccttc cggcggcgga atgtcgcgca ccagacgttc gagaacgtcc
    76261 tgcacgccaa cgccggtttt cgctgaacag cgcaccgcgt cagtggcgtc gatgccgacg
    76321 atatcttcaa tttcttccgc cacgcgttca ggatcggctg ccggcaggtc aatcttgttc
    76381 agtactggca ccacttcgag atccatttcc atggcggtgt agcagtttgc cagggtttgc
    76441 gcttccacgc cctgcccggc gtcgaccacc agcaatgcac cttcacaggc agccagcgaa
    76501 cgggaaactt cataggagaa gtctacgtgg cccggggtgt cgataaagtt aagctgatag
    76561 gtttcgccgt cagacgcttt gtagtccagc gtcacgcttt gcgctttgat ggtaatgcca
    76621 cgctcacgct caagatccat ggaatcgaga acctgcgcct ccatttcacg gtcagacagg
    76681 ccaccgcaga tctggataat acggtcagac agcgtcgatt taccgtggtc aatgtgagct
    76741 atgatcgaaa agttacgtat attcttcata aagtatgatt attgtgcctt aatgcccggt
    76801 taaccaggct tttagaagtc gctgttctga gcttaacgtc tgtattaata gaaacgccgc
    76861 attctacact acaacattga ggcgaggaaa tgttcatacc gtatggattg tggtatctgg
    76921 aaacgtcctc gcatttgtta tgcaaaatgc aacaaagcca gtgaaatcac tggctcgcgt
    76981 cttccgaaga tgtttcaaat cgcacaaggc caggcggcaa tgccacgctt aagatgatcg
    77041 gttgccattc tgcccgggca gcaaacttac gcgagtagcc gcgcgcaatc aggaacccgc
    77101 caataccacc gaggatcgca ccacataatg ctgcgacgtc agaagcaaag agtagctgaa
    77161 atagcgaagc gataaggaat aatcccacca gcggcgacat ataaaccagt aatgcggagc
    77221 taagcaggct gccttcggcg atccccaatt ccactttttg ccccggcact aacggttcat
    77281 cacagggtac gacaatggta tgcgtggttt gcgggccaag tttattcaac acgcggctac
    77341 cgcaaccggc gcgtgaagcg cagctgctgc atgaagcttt aacatcacaa ctgaccagcg
    77401 cctgcccgtt ttgccaggag acgacggtag cccactcttt gatcattgcg ctgccccgaa
    77461 cttaatattc tcggcaatgc gtttcgccgt ttgcggcggc agttcaccga caatggtgat
    77521 ttcggcgtta tcacgtacgc ttgtactgac ggttctgcgt ccggtgcgca acatctgatc
    77581 ggtgctcgat ggcgtagcgc ggttaacgtt taccgagaag ctgaataatc cgtcagaata
    77641 gagacgtgat tcgataggca tgttgtccat cgtcggtagc ggacgtcgac tactggaaac
    77701 ttcgctaaaa ccctgtggca accaggttgg cgtccagctg aatttagctt tttcacctac
    77761 gggaacagaa agcaacggcg gcaaatttgc cttcgccagc gtctgcatac tgctgctgat
    77821 atcctgattg acgttaaaag caatcacgcg aaattgttcc agcgtttcac catcgcgatc
    77881 aaggagatca acccgcatcg gtaatttcga ttcggtgtcc atccacacga tgtagctgta
    77941 gcgtgtacca tctcgggcaa ccacgcgaat gacttcgcaa agacgatcag caatacgcgt
    78001 gcgcccgacg gagataaagt cgtagtaagg agaaaggcgt ttgaaatcgg tatagataag
    78061 cgatggcaga gaatcaacaa tgtaatcgcc attaagcgtg aacggttcaa gtcccggttc
    78121 aaaatagctg atttcattgc cgcgctgtac cacttcccgg cgcgggccat ccatttgcaa
    78181 caattgtgca agaggacggt tatcgaggcg tgcatgtcga taacgcagag actcaacacc
    78241 ctgtttattg atgctgatga atgacagctc gtaattcagt gactgactgg ccaggttcat
    78301 ctgctgtaat aacgccccgg acgcgggagt ggccgaggcg ttagcagaga ataacaggct
    78361 acctgtcact aatgacatgg caaaccaaag ttgcttcatt actgcgattg cgttcctaaa
    78421 gtttgaattc ctggcacctg tacagcggct tgctgggttt gcgcctgctc aaactgaagc
    78481 tgttcagagt ggagtcggcg ttgcagttcg taatcctgca acattgcatt aatgcgacga
    78541 cgctgctcct gtacctgctg ctgttgacca ttgtttgcgg tcgcttcaga aggtactccc
    78601 aggcttaccg ggctggcttt acccatcatc ggcagtgtat taaataccgg cgtttcgggc
    78661 tgctgggacg tttcagattg tccattatag tgctggacgc caacgataac tgcaagcgat
    78721 acgcatgctg ctacgcccat ttgggtaagc tgtgccgccc acggacgtac tttctgccag
    78781 aatggcattt tctgccattg atgcggcgca ggctgggctt ccgggatcaa tgtcgccggt
    78841 tgacgtactg gctcttcttc aatggcggcc atcacgcgtg aagagatatc gaaatggagc
    78901 acctcgggag tatcaccccg cattgaatca cggattaagt gatagctttc ccaggttttc
    78961 tgcatttctg ggttatgagc cagttcgtta agcagctcac tatccagcgt ttcgccatcc
    79021 attaaagcgg aaagttgttc tttctgcatg cctaataccc ttatccagta tcccgctatc
    79081 gtcaacgcct gataagcggt tgaactttgt tatcaatagc ttccctcgct cggaagatac
    79141 gtgaacgcac cgtacctacc ggacaatcca tgatagcggc tatctcttca tagctcaggc
    79201 catccagctc ccgcaaggtt attgccatgc gcaaatcttc cgggagggac tcaatagttc
    79261 ggaaaactat ctgtctcagt tcttctgaca acattaagtt ctcagggttc gaaatttctt
    79321 tcaacgcgcc gccactttcg aagttttcag cttcaatggc atccacatca ctggaaggtg
    79381 gacgacgccc ctgagcaacc aggtaatttt tcgctgtatt tacagcaatc cgatacagcc
    79441 atgtataaaa agcgctatct ccccggaacg aatccagcgc acgataggct ttaataaaag
    79501 cttcttgtac cacatcggga acatcacccg acggcacata gcgggaaacc agactcgcca
    79561 ctttatgctg atagcgcact accagtaagt taaaggcttt ctgatctccc ttctggaccc
    79621 gttcaaccag gacctggtcc gttaactgct cgctcatccg aggtaaagtc tccccaaacc
    79681 aaatttccac gcgctatcga aacgccactc cattagctgc aatttgagca agcaaagggt
    79741 tagagtgtct cgtttttgta aagttccgta acgcatctgt ttttgtttgt catgctgtag
    79801 acggatcatt atctatcatt ataagtctac agaatctgaa catcgcatta tctgtgtaga
    79861 aatgcccatt taactgcctg aagagtaacc caacggcctt tttatttcac cacctaatcc
    79921 tccaccagcc agtaacttct ctttttctcg ccgccctgca tcagcgtgtt tagcaactgt
    79981 aacaaatatt aaaatagcag gtgtttattc gcacaacatg atgctatgct gaccaaacca
    80041 tgtttagtaa attaaacaaa gaaaatgaat actctccctg aacattcatg tgacgtgttg
    80101 attattggta gcggcgcagc cggactttca ctggcgctac gcctggctga ccagcatcag
    80161 gtcatcgttc taagtaaagg cccggtaacg gaaggttcaa cattttatgc ccagggcggt
    80221 attgccgccg tgtttgatga aactgacagc attgactcgc atgtggaaga cacattgatt
    80281 gccggggctg gtatttgcga tcgccatgca gttgaatttg tcgccagcaa tgcacgatcc
    80341 tgtgtgcaat ggttaattga ccagggggtt ttgtttgata cccacgttca accgaatggc
    80401 gaagaaagct accatctgac ccgtgaaggt ggacatagtc accgtcgtat tcttcatgcc
    80461 gccgacgcca ccggtagaga agtacaatcc acgctggtga gcaaggcgca gaaccatccg
    80521 aatattcgtg tgctggagcg cagcaacgcg gttgatctga ttgtctctga caaaattggc
    80581 ctgccgggca cgcgacgggt tgttggcgcg tgggtatgga accgaaataa agaaacggtg
    80641 gaaacctgcc acgcaaaagc agtggtactg gcaaccggcg gtgcgtcgaa ggtttatcag
    80701 tacaccacca atccggatat ttcttctggc gatggcattg ctatggcgtg gcgcgcgggc
    80761 tgtcgggttg ccaatctcga atttaatcag ttccacccta ccgcgctgta tcacccacag
    80821 gcacgcaatt tcctgttaac ggaagcactg cgcggcgaag gcgcttatct caagcgcccg
    80881 gatggcacgc gttttatgcc cgattttgat gagcgcggcg aactggcccc gcgcgatatt
    80941 gtcgcccgcg ccattgacca tgaaatgaaa cgcctcggcg cagattgtat gttcctcgac
    81001 atcagccata agcccgccga ttttattcgc cagcatttcc cgatgattta tgaaaaattg
    81061 ctcgggctgg ggattgatct cacacaagaa ccggtaccga ttgtgcctgc tgcacattat
    81121 acctgcggtg gtgtaatggt tgatgatcat gggcgtacgg acgtcgaggg cttgtatgcc
    81181 attggcgagg tgagttatac cggcttacac ggcgctaacc gcatggcctc gaattcattg
    81241 ctggagtgtc tggtctatgg ctggtcggcg gcagaagata tcaccagacg tatgcctgat
    81301 gcccacggcg tcagtacgtt accgccgtgg gatgaaagcc gcgttgagaa ccctgacgaa
    81361 cgggtagtaa ttcagcataa ctggcacaag ctacggctgt ttatgtggga ttacgttggc
    81421 attgtgcgca caacgaagcg cctggaacgc gccctgcggc ggataaccat gctccaacaa
    81481 gaaatagacg aatattacgc ccatttccgc gtctcaaata atttgctgga gctgcgtaat
    81541 ctggtacagg ttgccgagtt gattgttcgc tgcgcgatga tgcgcaaaga aagccggggg
    81601 ctgcatttta cgctggatta cccggaactg ctcacgcatt ccggtccatc gatcctttcc
    81661 cccggcaatc attacataaa cagataaaaa gcctgggtca gcgccgtata cgcttcggaa
    81721 tagttctggt ctggcccacg aatgactaag cgatcgctaa agcattctcc cgcctgcggg
    81781 gagaatgcca gcagcacccg atgcggcagt cgcgcttcgt tttccgccac atccgtccgc
    81841 aaacgtaaat gccagcccat gcttaatgcc agttcagtaa aaccattacc aatctgctct
    81901 ggcagcacca cgcagaaaaa tccctcttcg gtaatgcact ccgccgcaca ggtcagcagc
    81961 gatgggtgat caagcgtagt ggtatagcga gcctgttccc gttgaggtgt cgcgcactct
    82021 actccctgct gatagtaagg tgggttactg atgattaaat cgaagcgaac tgtctgctgt
    82081 gtgatccact gctgaatatc cgccgtatgg acgttaatcc gctctgccca cggggactgg
    82141 ttgatatttt cctgcgcctg cgcggcagct tcactttcca gctcaacggc atcaattatc
    82201 acgctgccac tggttcgctg cgccagcatc agtgccagca acccgctacc cgcgccgata
    82261 tcaaggcaac gtttcacccc agccaccggt gcccatgcgc ccagcaaaat gccatccgtt
    82321 cccactttca tcgcacagcg atcgtgagca acaaaaaact gtttaaaagt aaatccatta
    82381 cgacgaagca cggatgtaga ctgtgacatg aaaataaaac cttgcaggaa aaacggcgat
    82441 agcaccgggt gagaacaata cccgagaagc gatatccata caaacagatg aagattgcag
    82501 ccgtaacgtc tataatcagc gccccacaca gaggtagaac atgactgtaa cgactttttc
    82561 cgaacttgaa ctcgacgaaa gcctgctgga agccctccag gataaaggtt tcactcgccc
    82621 gaccgccatt caggctgccg ccattccgcc tgcgctcgat ggccgtgatg tactcggttc
    82681 tgcgccgaca ggcaccggta aaacggcggc gtatctgctg ccagcgttgc agcacctgct
    82741 cgatttccca cgtaagaaat ccggtccacc gcgtattttg atcctcaccc caactcgcga
    82801 gctggcgatg caggtggccg atcatgcccg cgaactggcg aaacatacgc atctggatat
    82861 cgccaccatc accggcggcg tagcctatat gaaccacgcg gaagtgttca gcgaaaatca
    82921 ggacatcgtg gtcgccacga ccggacgtct gctgcaatac ataaaagaag agaacttcga
    82981 ttgccgcgcg gttgaaacgc tgatcctcga cgaagcagac cgtatgctgg atatgggctt
    83041 cgctcaggat atcgaacata ttgctggcga aacgcgctgg cgtaaacaga ccctgctctt
    83101 ttcggcaacg ctggaaggcg atgcgattca ggactttgcc gagcgtctgc tggaagatcc
    83161 ggtggaggtt tctgccaatc cgtccacccg tgagcgcaaa aaaattcatc agtggtatta
    83221 ccgcgccgat gacctggaac ataaaaccgc attgctggtg catctgttaa aacagccgga
    83281 agcgacccgc tcaattgtgt ttgtgcgtaa gcgtgaacgt gtgcatgagc tggcaaactg
    83341 gctgcgcgaa gcgggcatca acaactgcta tctcgaaggt gagatggtac aaggcaagcg
    83401 taacgaagcg atcaagcgtt tgaccgaagg tcgcgtaaac gtactggttg caaccgatgt
    83461 tgccgcgcgc ggtatcgaca ttcctgacgt cagccacgtc tttaacttcg atatgccgcg
    83521 cagtggcgat acttatttgc accgtatcgg acgtaccgcg cgcgccggtc gtaaaggcac
    83581 cgcaatttcg ctggtggaag cccatgacca tttgctgctg ggcaaggttg gccgttacat
    83641 tgaagagccg attaaagctc gcgttattga tgagttacgc ccgaaaacgc gtgcgccaag
    83701 cgagaagcag accggcaagc catcgaagaa agtactggca aaacgcgccg agaagaaaaa
    83761 agctaaagag aaagagaaac cgcgggtgaa aaaacgccat cgcgacacca aaaatattgg
    83821 taagcgccgt aaaccaagcg gaacgggcgt gccaccgcaa acgacagaag agtaatctca
    83881 atgccgggtt taagcctggt attaaaagtg ctggcaaacg caaaactgcc tgatgcgcta
    83941 cgcttatcag gcctacgtgg ctcatgcaat atattgaatt tgcacgatct tgtaggccgg
    84001 ataaggcgtt cacgccgcat ccggcatgaa taaagcgcac tttgtcgaca agttaaatgc
    84061 cgggtttaat cccgacatta tttttatcca gccacaaaac tctcttcaac acactgaata
    84121 aaaatgtgca tcgccgggct aaccgctttt ccagcatgat gtgcgcacat tgccgtaatg
    84181 gtctgcgact gttcgccaaa aggcaattca attaattctc cgctctcaag ctctttttcc
    84241 accgcaaagc ggggcaagta actgacaccg atattcgccg cgacacaacg tttgatactt
    84301 tcaatgctta acagctcaat ggtgttttcc accgtgctcc gccgctggcg caatgtactt
    84361 tcaaagatct gccgaaagac acattgcggt tcgttgataa taaagctaca ggcgttatgt
    84421 cttcccggct cagtaaaatc gacatctgca atttgcggtg aagccaccag caccagcgat
    84481 tgttcaccca actctcgtcg gttcagggca tcatcattcc ctacgcgata aaagacgccg
    84541 acatcggcct catcattcag tagcgcatca cggatcacgt aacagttcag cgactgcaac
    84601 gataaacgca cttttggtgc gcgttgccga aaacgctgca acacctgcgg cattcggtaa
    84661 gagagcaacg tttcgcccga aacaacgcgt agttccccgt ccggatctga ctcctttttg
    84721 gcagcctcac gaagcgtatc catcactcgg gtaagttcat aaatgtgcgg cagcaacttt
    84781 tttccttcgc gggtaaggca catccgtcgg ccaattttct caaataactg gactgaaaac
    84841 tcctgctcaa gctgctgaat atgaaaagtc accgtcgatt gtgtacagca caatttttgc
    84901 gaagctcgca aaaaggaacc ctcttccacc acggttttaa gcgtaataaa acggcgcaga
    84961 tccataaccc caaacctatc gaaaatatcg aatctagaat ataaaaacat tcattttttt
    85021 aaatgttccg tgtcgggtac tgtctaccaa aacagaggag ataacaagtg acaccgaccc
    85081 ttttaagtgc tttttggact tacaccctga ttaccgctat gacgccagga ccgaacaata
    85141 ttctcgccct tagctctgct acgtcgcatg gatttcgtca aagtacccgc gtgctggcag
    85201 ggatgagtct gggatttttg attgtgatgt tactgtgtgc gggcatttca ttttcactgg
    85261 cagtgattga cccggcagcg gtacaccttt tgagttgggc gggggcggca tatattgtct
    85321 ggctggcgtg gaaaatcgcc accagcccaa caaaggaaga cggacttcag gcaaaaccaa
    85381 tcagcttttg ggccagcttt gctttgcagt ttgtgaacgt caaaatcatt ttgtacggtg
    85441 ttacggcact gtcgacgttt gttctgccgc aaacacaggc gctaagctgg gtagttggcg
    85501 tcagcgtttt gctggcgatg attgggacgt ttggcaatgt gtgctgggcg ctggcggggc
    85561 atctgtttca gcgattgttt cgccagtatg gtcgccagtt aaatatcgtg cttgccctgt
    85621 tgctggtcta ttgcgcggta cgcattttct attaacgaaa aaaagcggaa gaggtcgccc
    85681 tcttccgctt agtaacttgc tacttaagcc ttacaggctt tcagtaaagg tacgagcgat
    85741 aacgtcgcgc tgctgttccg gagtcagaga gttaaagcga actgcataac cggatacacg
    85801 gatggtcagc tgcggatatt tttccggatg cttaactgca tcttccagag tttcgcgacg
    85861 cagaacgtta acgttcaggt gttgaccacc ttcaacgcga acttctggtt tcacttctac
    85921 tggaacttca cggtattcaa tgtcacccag tttgcttact gcaaccactt catcttctgc
    85981 ataacctgct tttgcaacga tgcaacgcgc ttcgcctttt tcgctgtcca gcagccagaa
    86041 agagttcagc agatcgtcgt tagcggcttt agtaatctgg atacctgtaa tcatgtgatg
    86101 cctccccggc aaaattattt gatttgttca gcctgtcgcg gccaattggt aaaaccattg
    86161 ttgcttgagt gtatatatac tcctcaaaca cccttgaatc tttgatttaa atcaataaaa
    86221 accacacatc aagtattgtc gcaaatggat tttattgttt tacatcaact tatgcgggtg
    86281 tgaaatttta ccaatttaca tttttttgca ctcgtttaag tctaaaaaat gagcatgatt
    86341 ttgttctgta gaaagaagca gttaagctag gcggattgaa gattcgcagg agagcgagat
    86401 ggctaacgaa ttaacctggc atgacgtgct ggctgaagag aagcagcaac cctattttct
    86461 taataccctt cagaccgtcg ccagcgagcg gcagtccggc gtcactatct atccaccaca
    86521 aaaagatgtc tttaacgcgt tccgctttac cgagttgggt gacgttaaag tggtgattct
    86581 cggccaggat ccttatcacg gaccgggaca ggcgcatggt ctggcatttt ccgttcgtcc
    86641 cggcattgcc attcctccgt cattattgaa tatgtataaa gagctggaaa atactattcc
    86701 gggcttcacc cgccctaatc atggctatct cgaaagctgg gcgcgtcagg gcgttctgct
    86761 gctcaatact gtgttgacgg ttcgcgcagg tcaggcgcat tcccacgcca gcctcggttg
    86821 ggagacgttt actgataaag tcatcagcct gattaaccag caccgcgaag gcgtggtgtt
    86881 tttgttgtgg ggatcacatg cgcaaaagaa aggggcgatt atagataagc aacgccatca
    86941 tgtactgaaa gcaccgcatc cgtcgccgct ttcggcgcat cgtggattct ttggctgcaa
    87001 ccattttgtg ctggcaaatc agtggctgga acaacgtggc gagacgccga ttgactggat
    87061 gccagtatta ccggcagaga gtgagtaaat ttgcggggaa atgccggatg gcagagttgc
    87121 cacccggctg atttatcagg ctttattctg acgccaccat tcaccaagca aaacgccggt
    87181 tgcgacagag atattcagcc cggcaacgtt gcccgtaccg tcaatcttca cgcgcagatc
    87241 gttcggatcg cgtgcggcat ccggtaaccc ttcatattcc tgacccagca ccagtaccat
    87301 tttcgctggc agactagttt tgaacagcgg tttaccctgc tcgctggaag tggtcactac
    87361 ggtgtaacct gcctgacgga aatcatccag cacgttaaca atgttgtcgc cagtaatcgg
    87421 ctgaacgtgc tctgcgccgc cttctgcggt acggatagcc gcccccgact ccagcagtgc
    87481 cgcatcctgc accacgacac ctttcacgcc gaagtgcgcg cagctgcgca tcatgccacc
    87541 caggttatgc gggttagatt cgttttccag tgccagaacg caatcctgcg cgcctgcctg
    87601 gcttacccac tgctgcacgg ttgtaccgtt acgctttttg atcaagaagc aaacgccgcc
    87661 gtgatgttcc gtgcctgacg cttttgtcaa ttccgcttca tccaccacat ggtacgcttt
    87721 gcggtttgct gccatccagc gcaaggcttc tttaaaacgc ggcgttacac tctggataaa
    87781 ccaggcgcga acaatcgctt ccggacggct ctggaaaagt gcctgacatg cattttcgcc
    87841 gtagacgcgg gtttcttccg cacgctgacg acgcaacact tccggatcaa taaaactttt
    87901 accactgatg ccaccgtgat cggccttttc cggcgtctca tcacccggcg cgcgggaaac
    87961 cgtgcgccac ggcgaatctt cccacttgcg gtcacggggc tgactctgtt tgtcatcgcg
    88021 ggcggggcga cggccaccgt cagcacgaga ttttcctgga cgcccgcccc ctttcccggt
    88081 acgcgggttg tgggtacgtt tatcagaatc atcatcactg cggacataca tcactttgac
    88141 cttgccgctt ttacctttca tttcatcgtt catgcttttc tccaccagcg ctgcgcgaag
    88201 cgcgcagatt acccgaagtc cgcgcggttc gccatgattt cgtaccaaag cctgcgacta
    88261 tcatacctat tgaataaaac agattgttgt ctggaacaat gtccccgata atatgtaaca
    88321 tattagaaac ataccggcgt cgttgccgat aagtctcctt actcatcccg aggttagtta
    88381 tgaataccgt ttgtacccat tgtcaggcca tcaatcgcat tcccgacgat cggatcgaag
    88441 atgcggcaaa atgcggacgc tgcggtcacg acttgtttga cggagaggtg attaatgcga
    88501 ccggtgaaac actcgacaaa ttgctgaagg atgatctacc tgtggtgatc gacttctggg
    88561 caccgtggtg cggcccctgc cgtaatttcg caccaatttt tgaagatgtc gcgcaagagc
    88621 gtagcggtaa agtgcgcttt gtgaaagtga ataccgaagc tgaacgtgaa ttgagcagtc
    88681 gctttggaat tcgtagtata ccgacgatca tgattttcaa aaacggtcag gttgtcgaca
    88741 tgcttaatgg cgcagtaccg aaagcgccgt tcgatagctg gctgaacgaa tctctttaat
    88801 cttaccgggg cgcatcttgt gccccgtttt ctcctctgcg acaatggcgt tttttcgacg
    88861 ctctcttatg accgaaaacg ctgttctcca gttacgcgcc gagcgtattg cgcgcgcaac
    88921 acgtcctttt cttgcccgcg gtaatcgcgt tcgtcgctgc caacgctgtc ttttgccaga
    88981 gaaattatgt ctctgttcga caattacccc agcacaagca aaaagtcgct tctgtttgct
    89041 gatgttcgac accgagccaa tgaagcccag taataccggg cgtctgattg ctgatatttt
    89101 gcctgatacc gttgcgtttc aatggtcgcg taccgaaccc tcgcaagatt tactggatct
    89161 ggtacaaaac ccggactatc agccaatggt ggtctttccc gcttcgtatg ctgatgagca
    89221 acgggaagtg atcttcacac cacctgccgg taagccaccg ctgtttatca tgctcgatgg
    89281 tacctggccg gaagctcgca agatgtttcg taaaagtccg tatctggata atcttcccgt
    89341 catttccgtc gatctttccc ggctttctgc ctatcgcctg cgtgaagccc aggctgaagg
    89401 ccaatattgt actgccgagg tagccatcgc actgttagat atggccggcg ataccggggc
    89461 ggcagcaggg ttaggcgagc attttacccg ctttaaaaca cgctatctgg caggaaaaac
    89521 gcaacatctg ggtagcatca cagcagaaca gttagaaagc gtttaaaatc attcggtcac
    89581 ttctgcggga gaccggtatg agtcagcgag gactggaagc actactgcga ccaaaatcga
    89641 tagcggtaat tggcgcgtcg atgaaaccca atcgcgcagg ttacctgatg atgcgtaacc
    89701 tgctggcggg aggctttaac ggaccggtac tcccggtgac gccagcctgg aaagcggtgt
    89761 tgggtgtgtt ggcctggccg gatattgcca gcttgccctt tacacccgac cttgcggttt
    89821 tatgtaccaa tgccagccgt aatcttgctc ttctggaaga gctcggcgag aaaggctgta
    89881 aaacctgcat tattctttcc gccccggcat cgcaacacga agatctccgc gcctgcgccc
    89941 tgcgccataa catgcgcctg cttggaccaa acagtctggg tttactggct ccctggcaag
    90001 gtctgaatgc cagcttttcg cctgtgccga ttaaacgcgg caagctggcg tttatttcac
    90061 aatcggcagc cgtctccaac accatcctcg actgggcgca acagcgtgag atgggctttt
    90121 cctactttat tgcgctcggc gacagcctgg atatcgacgt tgatgaattg ctcgactatc
    90181 tggcgcgcga cagtaaaacc agcgccatcc tgctctatct cgaacagtta agcgacgcgc
    90241 gacgctttgt ttcggcagcc cgtagtgcct cgcgtaataa accgattctg gtgattaaaa
    90301 gcggacgtag cccggcggca cagcgactgc tcaacacgac ggcaggaatg gacccggcat
    90361 gggatgcggc tattcagcgt gccggtttgt tgcgggtaca ggatacccac gaactgtttt
    90421 cggcggtgga aacccttagc catatgcgcc cgctgcgtgg cgaccggttg atgattatca
    90481 gcaacggtgc tgcgcctgcc gcgctggcgc tggatgcctt atggtcacgc aatggcaagc
    90541 tggcaacgct aagcgaagaa acctgccaga aactgcgcga tgcactgcca ggacatgtgg
    90601 caatctctaa cccgctcgat ctgcgcgatg acgccagcag tgagcactat attaaaacgc
    90661 tggacattct gctccacagc caggattttg atgcgctgat ggttattcat tcgcccagcg
    90721 ccgctgctcc cgcaacagaa agcgcgcaag tattaattga agcggtaaag catcatcccc
    90781 gcagcaagta tgtttctctg ctgacgaact ggtgtggcga acattcctcg caagaggcac
    90841 gacgattatt cagcgaagcc gggctgccga cctaccgtac cccggaagga accatcactg
    90901 cttttatgca tatggtggag taccggcgta atcagaagca actacgcgaa acgccggcgt
    90961 tgcccagcaa tctgacctcc aataccgcag aagcgcatct tctgttgcaa caggcgattg
    91021 ccgaaggagc aacgtcactc gatacccatg aagttcagcc tatcctgcaa gcatatggca
    91081 tgaacacgct ccccacctgg attgccagcg atagcaccga agcggtgcat attgccgaac
    91141 agattggtta tccggtggcg ctgaaattgc gttcgccgga tattccacat aaatcggaag
    91201 ttcagggcgt catgctctac ctgcgtacag ccaatgaagt ccagcaagcg gcaaacgcca
    91261 ttttcgatcg cgtaaaaatg gcctggccgc aggcacgggt ccacggcctg ttggtgcaaa
    91321 gtatggctaa ccgtgctggt gctcaggagt tgcgggttgt ggttgagcac gatccggttt
    91381 tcgggccgtt gatcatgctg ggcgaaggcg gtgtggagtg gcgtcctgaa gatcaagccg
    91441 tcgtcgcgtt accgccgctg aacatgaacc tggcccgcta tctggttatt caggggatca
    91501 aaagtaaaaa gattcgtgcg cgcagtgcgc tacgcccatt ggatgttgca ggcttgagcc
    91561 agcttctggt gcaggtttcc aacttgattg tcgattgccc ggaaattcag cgtctggata
    91621 ttcatccttt gctggcttct ggcagtgaat ttaccgcgct ggatgtcacg ctggatatct
    91681 cgccgtttga aggcgataac gagagtcggc tggcagtgcg cccttatccg catcagctgg
    91741 aagaatgggt agaattgaaa aacggtgaac gctgcttgtt ccgcccgatt ttgccagaag
    91801 atgagccaca acttcagcaa ttcatttcgc gagtcaccaa agaagatctt tattaccgct
    91861 actttagcga gatcaacgaa tttacccatg aagatttagc caacatgaca cagatcgact
    91921 acgatcggga aatggcgttt gtagcggtac gacgtattga tcaaacggaa gagatcctcg
    91981 gcgtcacgcg tgcgatttcc gatcctgata acatcgatgc cgaatttgct gtgctggttc
    92041 gctcggatct caaagggtta ggcttaggtc gacgcttaat ggaaaagttg attacctata
    92101 cgcgagatca cggactacaa cgtctgaatg gtattacgat gccaaacaat cgtggcatgg
    92161 tggcgctagc ccgcaagctc gggtttaacg ttgatatcca gctcgaagag gggatcgttg
    92221 ggcttacgct aaatcttgcc caacgcgagg aatcatgagt aaggtactgg aaatgttgac
    92281 cacttaatcg ggactggtgt tattattgcc cgcttatgtc gtctgcattg cacagaggac
    92341 ccttcaatga acagagaaga aatgcactgt gatgttgtca aaatttaagc gtaataaaca
    92401 tcaacaacac cttgcccaac tacccaagat ttctcaatca gttgatgatg tcgatttctt
    92461 ttacgctccc gccgacttcc gggagacgct gctggaaaaa atagccagcg cgaagcagcg
    92521 catttgcatt gtcgccctgt atctcgaaca ggatgacggt ggcaaaggca ttctgaacgc
    92581 gttgtatgaa gctaaaaggc agcgtccgga actggatgtg cgggtgctgg tcgactggca
    92641 tcgtgcacaa cgtggacgca ttggcgctgc ggcatctaac actaacgctg actggtactg
    92701 ccgcatggcg caggaaaatc cgggcgtaga tgttccggtt tatggcgttc caatcaatac
    92761 ccgtgaagcc cttggtgttc tgcactttaa aggctttatc atcgacgata gcgtacttta
    92821 tagcggtgcc agcctgaacg atgtttacct gcatcagcac gataaatatc gctacgaccg
    92881 ttatcatctg atccgtaacc gtaagatgtc agacattatg tttgaatggg ttacacagaa
    92941 tattatgaat ggccgcggcg ttaatcgtct ggatgatgtt aatcggccaa aaagcccgga
    93001 aatcaagaac gatattcgtc tgttccgcca ggagctgcgt gatgccgctt atcatttcca
    93061 gggcgatgcc gacaacgatc agctttccgt aacgccgcta gtggggctgg ggaaatcgag
    93121 tctgttgaac aagaccattt tccatcttat gccttgtgcc gagcagaaac taaccatctg
    93181 tacgccatac ttcaacctgc cagcaatcct tgtgcgcaat attatccagt tgctgcgcga
    93241 agggaaaaag gtcgaaatta ttgttggtga taaaaccgcg aacgacttct acattccgga
    93301 agatgaacct ttcaagataa ttggcgcatt gccttatctc tatgagatca atctgcgtcg
    93361 tttcctgagc cgtttgcagt attacgtcaa tactgaccag ctagtggttc ggttatggaa
    93421 agatgacgac aacacctatc acctaaaagg gatgtgggtt gatgataagt ggatgttgat
    93481 caccggtaat aacctgaacc cgcgcgcctg gcgtctggat ctggaaaacg ccattttgat
    93541 ccacgatcca caactggagc ttgcgccgca gcgagagaaa gaactggagc tgatccgcga
    93601 gcataccacc atcgttaagc actatcgcga tctgcaaagt attgccgatt atccggtgaa
    93661 ggttcgtaaa ctcatccgcc gtttgcgccg tatccgcatc gaccgattaa ttagccgcat
    93721 cctgtaatca caaccccgtc ctgtacgggg tttgtttttt ggaggccacg ttttgcgtat
    93781 tctttttgtc tgttcgctat tgttactttc tggatgcagc catatggcta acgatagctg
    93841 gagcgggcag gataaagctc aacactttat cgcctcggcg atgctttccg ccgccggaaa
    93901 tgaatattca cagcatcagg ggatgagccg ggatcgcagt gccatgtttg gattgatgtt
    93961 ctctgtcagt ttgggggcgt caaaagagct ttgggatagc cgccccgaag ggagcggctg
    94021 gagctggaag gatttggcct gggatgtcgc cggtgcaagc accggctata ccatctggca
    94081 actgacccgt cactaaagac gcatcccctt ccctttgcga tgtagcatca aagaaaccag
    94141 aaacgccacc acggccatca aggtcacata ccagaagaag gctgtttcca ttcctattga
    94201 tttcagcgac aacgctacgt actccgccga accaccaaat atagcattag cgaccgcata
    94261 tgacagacca acgcctaatg cgcgaacctg tgccgggaac atctcagcct tcagtattcc
    94321 actgattgat gtataaaaac tcactatcag cagggcacac atcaccagac caaaagcggc
    94381 ataaggcgag gaaacgtttt gcaatgctga gagaatagga acggtaaaaa tggctgccag
    94441 cgaaccgaaa cataacattg aggtacggcg accaatctta tccgacagcg cgccaatgag
    94501 tggttgaata agcatgaata caaacaatgc ggcagtcata atgccactcg ccacgttggc
    94561 atgcattccc gcagtattta ccagatactt ctgcatataa gtggtgaagg tatagaaaca
    94621 aagggagccc gcagcggtaa aaccgagaac catgatgaat gcacggcgat tgcgccataa
    94681 tcctttcaga gatccagctt cttttaaagc gcgcgtttct tgttgcgaag tttcatctaa
    94741 ctgacgacgt aaccacaacg ccacaacagc taacacagct cctaacgcga aaggaatacg
    94801 ccatccccac tctctgagtg cagcgtcttc catggtgtgt tgtaaaacca cgacaaccag
    94861 tagggctagc agttgtccgc cgatcaacgt cacatactga aatgatgcgt aaaaaccttt
    94921 gcgcccttca acggcaactt cactcatata ggtggcgctg gtgccatatt ctccgccaac
    94981 agataatccc tgaaataaac gcgcgagaag caataatgcc ggagcccacg tacctatagt
    95041 ttcataacct gggaggcagg cgataaccag cgatccgaaa cacatcatac acaccgataa
    95101 cagcatcgat tttttgcgac catgtttatc ggctatgcgg ccaaatagcc aaccgcctat
    95161 tgggcgcatc aggaatcccg cagcaaaaac acctgctgtt tgtagtagtt gagtcgtcgt
    95221 gttcccggaa gggaagaaaa tgtgggcaaa gtagagtgaa cagaacgagt agacatagaa
    95281 atcgaaccac tcgaccagat tacctgaaga ggcccccaca atcgcccaaa tgcggcgacg
    95341 agtatcacta cttgtcagtt tgctgtctgc cgttacagta ctttcagcca tgccattatg
    95401 tctcctgccg taatccgatg cttttgtcgg tcgcttttgt ttatttcttt tggtaaagaa
    95461 aatgttatac atttgttgcg tatcattatg caaccttaac catgaattta gttagcagga
    95521 aaatggttat tgaggagctt aaggataaat ttctggtgag gagaacacgt atggagatgg
    95581 gcaagttggg gaagccgtat tcgctgctga atctggcata tgtgggagta taagacgcgc
    95641 agcgtcgcat caggcatttt tttctgcgcc aacgcaaaaa ggccatcgtg actgatggcc
    95701 tttcgcttaa tttgatgcct ggcagttccc tactctcgca tggggagacc ccacactacc
    95761 atcggcgcta cggcgtttca cttctgagtt cggcatgggg tcaggtggga ccaccgcgct
    95821 a
//
LOCUS       DAZGSP010000003        95027 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZGSP010000003 DAZGSP010000000
VERSION     DAZGSP010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 95027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.003"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(63..1613)
                     /gene="cueO"
                     /locus_tag="ACHK74_000217"
     CDS             complement(63..1613)
                     /gene="cueO"
                     /locus_tag="ACHK74_000217"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFP0219103.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQHGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTVAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            1779..2126
                     /gene="yacC"
                     /locus_tag="ACHK74_000218"
     CDS             1779..2126
                     /gene="yacC"
                     /locus_tag="ACHK74_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFP0219104.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            2232..3098
                     /gene="speE"
                     /locus_tag="ACHK74_000219"
     CDS             2232..3098
                     /gene="speE"
                     /locus_tag="ACHK74_000219"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFP0219105.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLTHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            3114..3908
                     /gene="speD"
                     /locus_tag="ACHK74_000220"
     CDS             3114..3908
                     /gene="speD"
                     /locus_tag="ACHK74_000220"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFP0219106.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(3946..4308)
                     /gene="yacL"
                     /locus_tag="ACHK74_000221"
     CDS             complement(3946..4308)
                     /gene="yacL"
                     /locus_tag="ACHK74_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFP0219107.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(4484..7081)
                     /gene="acnB"
                     /locus_tag="ACHK74_000222"
     CDS             complement(4484..7081)
                     /gene="acnB"
                     /locus_tag="ACHK74_000222"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFP0219108.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            7436..9289
                     /gene="yacH"
                     /locus_tag="ACHK74_000223"
     CDS             7436..9289
                     /gene="yacH"
                     /locus_tag="ACHK74_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFP0219109.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVTQTVTAPVIPSN
                     TVSTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQRQ
                     AVREKMQTNPQIRQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSAVRERLSERGARRQER"
     gene            complement(9360..10784)
                     /gene="lpdA"
                     /locus_tag="ACHK74_000224"
     CDS             complement(9360..10784)
                     /gene="lpdA"
                     /locus_tag="ACHK74_000224"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFP0219110.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(11109..13001)
                     /gene="aceF"
                     /locus_tag="ACHK74_000225"
     CDS             complement(11109..13001)
                     /gene="aceF"
                     /locus_tag="ACHK74_000225"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFP0219111.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGSDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(13016..15679)
                     /gene="aceE"
                     /locus_tag="ACHK74_000226"
     CDS             complement(13016..15679)
                     /gene="aceE"
                     /locus_tag="ACHK74_000226"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFP0219112.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(15840..16604)
                     /gene="pdhR"
                     /locus_tag="ACHK74_000227"
     CDS             complement(15840..16604)
                     /gene="pdhR"
                     /locus_tag="ACHK74_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFP0219113.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            17145..18518
                     /gene="aroP"
                     /locus_tag="ACHK74_000228"
     CDS             17145..18518
                     /gene="aroP"
                     /locus_tag="ACHK74_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFP0219114.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(18561..19415)
                     /gene="ampE"
                     /locus_tag="ACHK74_000229"
     CDS             complement(18561..19415)
                     /gene="ampE"
                     /locus_tag="ACHK74_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFP0219115.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(19412..19963)
                     /gene="ampD"
                     /locus_tag="ACHK74_000230"
     CDS             complement(19412..19963)
                     /gene="ampD"
                     /locus_tag="ACHK74_000230"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFP0219116.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            20051..20944
                     /gene="nadC"
                     /locus_tag="ACHK74_000231"
     CDS             20051..20944
                     /gene="nadC"
                     /locus_tag="ACHK74_000231"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFP0219117.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            21147..21587
                     /gene="ppdD"
                     /locus_tag="ACHK74_000232"
     CDS             21147..21587
                     /gene="ppdD"
                     /locus_tag="ACHK74_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFP0219118.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTHYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            21597..22982
                     /gene="gspE"
                     /locus_tag="ACHK74_000233"
     CDS             21597..22982
                     /gene="gspE"
                     /locus_tag="ACHK74_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFP0219119.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            22972..24174
                     /gene="hofC"
                     /locus_tag="ACHK74_000234"
     CDS             22972..24174
                     /gene="hofC"
                     /locus_tag="ACHK74_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFP0219120.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPCWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(24209..25252)
                     /gene="guaC"
                     /locus_tag="ACHK74_000235"
     CDS             complement(24209..25252)
                     /gene="guaC"
                     /locus_tag="ACHK74_000235"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFP0219121.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(25408..25452)
                     /locus_tag="ACHK74_000236"
     CDS             complement(25408..25452)
                     /locus_tag="ACHK74_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219122.1"
                     /translation="MIIKTLKMSARKRN"
     gene            25477..26097
                     /gene="coaE"
                     /locus_tag="ACHK74_000237"
     CDS             25477..26097
                     /gene="coaE"
                     /locus_tag="ACHK74_000237"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFP0219123.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            26097..26840
                     /gene="zapD"
                     /locus_tag="ACHK74_000238"
     CDS             26097..26840
                     /gene="zapD"
                     /locus_tag="ACHK74_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFP0219124.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            26850..27047
                     /gene="yacG"
                     /locus_tag="ACHK74_000239"
     CDS             26850..27047
                     /gene="yacG"
                     /locus_tag="ACHK74_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFP0219125.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(27147..27536)
                     /gene="mutT"
                     /locus_tag="ACHK74_000240"
     CDS             complement(27147..27536)
                     /gene="mutT"
                     /locus_tag="ACHK74_000240"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFP0219126.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(27596..30301)
                     /gene="secA"
                     /locus_tag="ACHK74_000241"
     CDS             complement(27596..30301)
                     /gene="secA"
                     /locus_tag="ACHK74_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFP0219127.1"
                     /translation="MLIKLLTKIFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTVEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(30363..30875)
                     /gene="secM"
                     /locus_tag="ACHK74_000242"
     CDS             complement(30363..30875)
                     /gene="secM"
                     /locus_tag="ACHK74_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFP0219128.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(31106..32023)
                     /gene="lpxC"
                     /locus_tag="ACHK74_000243"
     CDS             complement(31106..32023)
                     /gene="lpxC"
                     /locus_tag="ACHK74_000243"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFP0219129.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(32124..33275)
                     /gene="ftsZ"
                     /locus_tag="ACHK74_000244"
     CDS             complement(32124..33275)
                     /gene="ftsZ"
                     /locus_tag="ACHK74_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP0219130.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(33336..34598)
                     /gene="ftsA"
                     /locus_tag="ACHK74_000245"
     CDS             complement(33336..34598)
                     /gene="ftsA"
                     /locus_tag="ACHK74_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFP0219131.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(34595..35425)
                     /gene="ftsQ"
                     /locus_tag="ACHK74_000246"
     CDS             complement(34595..35425)
                     /gene="ftsQ"
                     /locus_tag="ACHK74_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFP0219132.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(35427..36347)
                     /gene="ddlB"
                     /locus_tag="ACHK74_000247"
     CDS             complement(35427..36347)
                     /gene="ddlB"
                     /locus_tag="ACHK74_000247"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP0219133.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(36340..37815)
                     /gene="murC"
                     /locus_tag="ACHK74_000248"
     CDS             complement(36340..37815)
                     /gene="murC"
                     /locus_tag="ACHK74_000248"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFP0219134.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(37869..38936)
                     /gene="murG"
                     /locus_tag="ACHK74_000249"
     CDS             complement(37869..38936)
                     /gene="murG"
                     /locus_tag="ACHK74_000249"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFP0219135.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(38933..40177)
                     /gene="ftsW"
                     /locus_tag="ACHK74_000250"
     CDS             complement(38933..40177)
                     /gene="ftsW"
                     /locus_tag="ACHK74_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFP0219136.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(40177..41493)
                     /gene="murD"
                     /locus_tag="ACHK74_000251"
     CDS             complement(40177..41493)
                     /gene="murD"
                     /locus_tag="ACHK74_000251"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFP0219137.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(41496..42578)
                     /gene="mraY"
                     /locus_tag="ACHK74_000252"
     CDS             complement(41496..42578)
                     /gene="mraY"
                     /locus_tag="ACHK74_000252"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFP0219138.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(42572..43930)
                     /gene="murF"
                     /locus_tag="ACHK74_000253"
     CDS             complement(42572..43930)
                     /gene="murF"
                     /locus_tag="ACHK74_000253"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFP0219139.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(43927..45414)
                     /gene="murE"
                     /locus_tag="ACHK74_000254"
     CDS             complement(43927..45414)
                     /gene="murE"
                     /locus_tag="ACHK74_000254"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFP0219140.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(45401..47167)
                     /gene="ftsI"
                     /locus_tag="ACHK74_000255"
     CDS             complement(45401..47167)
                     /gene="ftsI"
                     /locus_tag="ACHK74_000255"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFP0219141.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(47183..47548)
                     /gene="ftsL"
                     /locus_tag="ACHK74_000256"
     CDS             complement(47183..47548)
                     /gene="ftsL"
                     /locus_tag="ACHK74_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFP0219142.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(47545..48486)
                     /gene="rsmH"
                     /locus_tag="ACHK74_000257"
     CDS             complement(47545..48486)
                     /gene="rsmH"
                     /locus_tag="ACHK74_000257"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFP0219143.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(48488..48946)
                     /gene="mraZ"
                     /locus_tag="ACHK74_000258"
     CDS             complement(48488..48946)
                     /gene="mraZ"
                     /locus_tag="ACHK74_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFP0219144.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(49548..50552)
                     /gene="cra"
                     /locus_tag="ACHK74_000259"
     CDS             complement(49548..50552)
                     /gene="cra"
                     /locus_tag="ACHK74_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFP0219145.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(50634..50720)
                     /locus_tag="ACHK74_000260"
     CDS             complement(50634..50720)
                     /locus_tag="ACHK74_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFP0219146.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(50732..51223)
                     /gene="ilvN"
                     /locus_tag="ACHK74_000261"
     CDS             complement(50732..51223)
                     /gene="ilvN"
                     /locus_tag="ACHK74_000261"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP0219147.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(51226..52950)
                     /gene="ilvI"
                     /locus_tag="ACHK74_000262"
     CDS             complement(51226..52950)
                     /gene="ilvI"
                     /locus_tag="ACHK74_000262"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFP0219148.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            53069..53113
                     /locus_tag="ACHK74_000263"
     CDS             53069..53113
                     /locus_tag="ACHK74_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219149.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(53268..54212)
                     /gene="leuO"
                     /locus_tag="ACHK74_000264"
     CDS             complement(53268..54212)
                     /gene="leuO"
                     /locus_tag="ACHK74_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFP0219150.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLER
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            54872..54961
                     /gene="leuL"
                     /locus_tag="ACHK74_000265"
     CDS             54872..54961
                     /gene="leuL"
                     /locus_tag="ACHK74_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFP0219151.1"
                     /translation="MTHIVRFIGLLLLLNASSLRGRRVSGIQH"
     gene            55054..56625
                     /gene="leuA"
                     /locus_tag="ACHK74_000266"
     CDS             55054..56625
                     /gene="leuA"
                     /locus_tag="ACHK74_000266"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFP0219152.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            56625..57716
                     /gene="leuB"
                     /locus_tag="ACHK74_000267"
     CDS             56625..57716
                     /gene="leuB"
                     /locus_tag="ACHK74_000267"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFP0219153.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEESI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            57719..59119
                     /gene="leuC"
                     /locus_tag="ACHK74_000268"
     CDS             57719..59119
                     /gene="leuC"
                     /locus_tag="ACHK74_000268"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFP0219154.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            59130..59735
                     /gene="leuD"
                     /locus_tag="ACHK74_000269"
     CDS             59130..59735
                     /gene="leuD"
                     /locus_tag="ACHK74_000269"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFP0219155.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(59784..60962)
                     /gene="setA"
                     /locus_tag="ACHK74_000270"
     CDS             complement(59784..60962)
                     /gene="setA"
                     /locus_tag="ACHK74_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFP0219156.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(61064..61195)
                     /gene="sgrT"
                     /locus_tag="ACHK74_000271"
     CDS             complement(61064..61195)
                     /gene="sgrT"
                     /locus_tag="ACHK74_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFP0219157.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            61284..62939
                     /gene="sgrR"
                     /locus_tag="ACHK74_000272"
     CDS             61284..62939
                     /gene="sgrR"
                     /locus_tag="ACHK74_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFP0219158.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIVRQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      62973..63072
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            63103..64086
                     /gene="thiB"
                     /locus_tag="ACHK74_000273"
     CDS             63103..64086
                     /gene="thiB"
                     /locus_tag="ACHK74_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFP0219159.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            64062..65672
                     /gene="thiP"
                     /locus_tag="ACHK74_000274"
     CDS             64062..65672
                     /gene="thiP"
                     /locus_tag="ACHK74_000274"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFP0219160.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            65656..66354
                     /gene="thiQ"
                     /locus_tag="ACHK74_000275"
     CDS             65656..66354
                     /gene="thiQ"
                     /locus_tag="ACHK74_000275"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFP0219161.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(66468..67232)
                     /gene="yabI"
                     /locus_tag="ACHK74_000276"
     CDS             complement(66468..67232)
                     /gene="yabI"
                     /locus_tag="ACHK74_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP0219162.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(67318..68196)
                     /gene="araC"
                     /locus_tag="ACHK74_000277"
     CDS             complement(67318..68196)
                     /gene="araC"
                     /locus_tag="ACHK74_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFP0219163.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            68535..70235
                     /gene="araB"
                     /locus_tag="ACHK74_000278"
     CDS             68535..70235
                     /gene="araB"
                     /locus_tag="ACHK74_000278"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFP0219164.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLSWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            70246..71748
                     /gene="araA"
                     /locus_tag="ACHK74_000279"
     CDS             70246..71748
                     /gene="araA"
                     /locus_tag="ACHK74_000279"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFP0219165.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            71917..72612
                     /gene="araD"
                     /locus_tag="ACHK74_000280"
     CDS             71917..72612
                     /gene="araD"
                     /locus_tag="ACHK74_000280"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP0219166.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPAIGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            72687..75038
                     /gene="polB"
                     /locus_tag="ACHK74_000281"
     CDS             72687..75038
                     /gene="polB"
                     /locus_tag="ACHK74_000281"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFP0219167.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RVLVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            75203..78109
                     /gene="rapA"
                     /locus_tag="ACHK74_000282"
     CDS             75203..78109
                     /gene="rapA"
                     /locus_tag="ACHK74_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFP0219168.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQIMESLDQAGWRLDALRLIVVTHQ"
     gene            78121..78780
                     /gene="rluA"
                     /locus_tag="ACHK74_000283"
     CDS             78121..78780
                     /gene="rluA"
                     /locus_tag="ACHK74_000283"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFP0219169.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(78897..79712)
                     /gene="djlA"
                     /locus_tag="ACHK74_000284"
     CDS             complement(78897..79712)
                     /gene="djlA"
                     /locus_tag="ACHK74_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFP0219170.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            79967..82321
                     /gene="lptD"
                     /locus_tag="ACHK74_000285"
     CDS             79967..82321
                     /gene="lptD"
                     /locus_tag="ACHK74_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFP0219171.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            82374..83660
                     /gene="surA"
                     /locus_tag="ACHK74_000286"
     CDS             82374..83660
                     /gene="surA"
                     /locus_tag="ACHK74_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFP0219172.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            83660..84649
                     /gene="pdxA"
                     /locus_tag="ACHK74_000287"
     CDS             83660..84649
                     /gene="pdxA"
                     /locus_tag="ACHK74_000287"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFP0219173.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADTTLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLGVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            84646..85467
                     /gene="rsmA"
                     /locus_tag="ACHK74_000288"
     CDS             84646..85467
                     /gene="rsmA"
                     /locus_tag="ACHK74_000288"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFP0219174.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            85470..85847
                     /gene="apaG"
                     /locus_tag="ACHK74_000289"
     CDS             85470..85847
                     /gene="apaG"
                     /locus_tag="ACHK74_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFP0219175.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            85854..86696
                     /gene="apaH"
                     /locus_tag="ACHK74_000290"
     CDS             85854..86696
                     /gene="apaH"
                     /locus_tag="ACHK74_000290"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFP0219176.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(86774..87253)
                     /gene="folA"
                     /locus_tag="ACHK74_000291"
     CDS             complement(86774..87253)
                     /gene="folA"
                     /locus_tag="ACHK74_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFP0219177.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(87446..89308)
                     /gene="kefC"
                     /locus_tag="ACHK74_000292"
     CDS             complement(87446..89308)
                     /gene="kefC"
                     /locus_tag="ACHK74_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFP0219178.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFVVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(89301..89831)
                     /gene="kefF"
                     /locus_tag="ACHK74_000293"
     CDS             complement(89301..89831)
                     /gene="kefF"
                     /locus_tag="ACHK74_000293"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFP0219179.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSVPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(89939..91270)
                     /gene="yaaU"
                     /locus_tag="ACHK74_000294"
     CDS             complement(89939..91270)
                     /gene="yaaU"
                     /locus_tag="ACHK74_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0219180.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRYFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(91328..91615)
                     /gene="fixX"
                     /locus_tag="ACHK74_000295"
     CDS             complement(91328..91615)
                     /gene="fixX"
                     /locus_tag="ACHK74_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFP0219181.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(91612..92898)
                     /gene="fixC"
                     /locus_tag="ACHK74_000296"
     CDS             complement(91612..92898)
                     /gene="fixC"
                     /locus_tag="ACHK74_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP0219182.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(92949..93890)
                     /gene="fixB"
                     /locus_tag="ACHK74_000297"
     CDS             complement(92949..93890)
                     /gene="fixB"
                     /locus_tag="ACHK74_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFP0219183.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGMFDAAQPDAS
                     RTGETHTMEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKDNIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(93905..94675)
                     /gene="fixA"
                     /locus_tag="ACHK74_000298"
     CDS             complement(93905..94675)
                     /gene="fixA"
                     /locus_tag="ACHK74_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFP0219184.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVIAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT        22794 a        26324 c        23937 g        21972 t
ORIGIN      
        1 tttgcgtttg tcatcagttt aatgcccgga gagatccggg catatttccg aatacggtct
       61 ttttataccg taaaccctaa catcatcccc gtatcttcat gctccagcag atggcagtgc
      121 gccatataag catgttcttt cggtgcatcg tgattaaact tcaccagcac ttcgctgaca
      181 ttaccttcta ccttaacggt atctttccag cccgcgcgat gagccgctgg cggtttgcca
      241 ttttctgaca agatacggaa ctgcgtgccg tgaatatgga acggatgcag catcatgtca
      301 cccacgccag agataaccca acgttcgtac tgccctttcg ccgccgcaaa catcggctta
      361 ttcatatcaa acgcctgacc gttgatttta ttggcatggt ggaaatcgaa cttcccgccg
      421 tggttcatat gattcatatt gccgtgcccc atatggccca tcatctggct gtgatccatc
      481 ccggccatcg cctgatcgcc atatttctcc atcagcatct gcatacccat catatcgagc
      541 atcgggtcca tagaaagttg cagcttgcgt accgtcagcc cttccagcga aggtaacgca
      601 ggcaggctac ttaatgtgtc tggcaaagca ccggaggcac taatagcaat cggctgaatc
      661 cgcattaccg gatgcggctt gtcaaacggc gcaatcgcca tccccatctg gctgaccggc
      721 agcgtcacca ggtcaaaggg tttgttgtcg ttaacctcca ccagcacttc aaaacgctcg
      781 cccatcagca ccggcagctc gttcaccttc accggttcag gtagcagacc accgtcgctg
      841 gcaatcacat aaagcgggcg attgtcgctg gtggcgaaat tgagcgagcg ggcattacag
      901 ccattgagca aacgcaggcg cagccaacca cgcggggcag cgtgttgcgg gtagattgca
      961 ccgttggtca gcaacgtatc gccaaaccag cccacggcga cggtcatcac atccagttga
     1021 taatcaatct gcccgtcggc gctaaatttc ttatcctgaa cgatcaccgg aacatcatcg
     1081 ataccccact gttttggcag cattaatttc aggatctcgt catcttcaat taccaccaac
     1141 ccagccaacc ccatcgccac ctgtcgcccg gtcttgccat gttgatgtgg atggaaccag
     1201 caggtagcgg caggttgatc aacgttcaac gtcaccgagc gcttgccacc tggcggaata
     1261 attccctgcg ggccgccgtc cacttcaccc ggtacttcca gcccgtgcca gtgcaacgtc
     1321 gtctcttccg tcagttggtt atagatatca accgttaccg ctttgccgtg ctgtaatttc
     1381 accgccggcc ccagcagatt gccgttatag ccccaggtag ttgcagtttt cccgccaaag
     1441 gtggactggc ctgcgccaat agttaactga atgcgattac gggcatcggt cgtgagcaaa
     1501 tcagggatcg gtaacgttgg gcgttccgcc gcaaagactg cgcggctcca cagcggcaag
     1561 gctgaagcca cacccagcgc gacggaatat tttaagaaat cacggcgttg catagttatt
     1621 tccttattct taagcaggcg aaacaaaatc aaacgttgag catagtcctt ccccttacgg
     1681 gaaggtcaag ccgcagacac aatttaatcg acaataatgg tcgtcgcctc gctcgcagtg
     1741 tgctaacgtt tatcttcttt aagcccctgg tagaagcaat gaagacgttt ttcagaacag
     1801 tgttattcgg cagcctgatg gccgtctgcg caaacagtta cgcgctcagc gagtctgaag
     1861 ccgaagatat ggccgattta acggcagttt ttgtctttct gaagaacgat tgtggttacc
     1921 agaacttacc taacgggcaa attcgtcgcg cactggtctt tttcgcccag caaaaccagt
     1981 gggatctcag taattacgac accttcgaca tgaaagccct cggtgaagac agctaccgcg
     2041 atctcagcgg cattggcatt cccgtcgcta aaaagtgcaa agccctggct cgcgattcct
     2101 taagcctgct tgcctacgtc aaataatccc tgataccttt ttgtagaaat attgaccgtg
     2161 catccgcggt caatgttagc tattatgttg cgcccttttt ttacgggtgt taacaaagga
     2221 ggtatcaacc catggccgaa aaaaaacagt ggcatgaaac gctacacgac cagtttgggc
     2281 agtactttgc ggtagataac gttctgtatc atgaaaagac cgatcaccag gatctgatca
     2341 tttttgagaa cgctgcattt ggtcgcgtaa tggcgctgga tggcgtagta caaaccaccg
     2401 agcgcgacga gtttatctat catgagatga tgactcatgt tccgctactg acccacggtc
     2461 acgcgaaaca tgtgctgatt atcggcggcg gcgacggtgc catgctgcgt gaagtaaccc
     2521 gacataaaaa cgttgagtca atcacgatgg tggaaatcga tgcgggtgtc gtatcgttct
     2581 gccgtcagta tctacccaac cataacgccg gtagctacga cgatccgcgc tttaagctgg
     2641 tgatcgatga tggcgtcaat ttcgttaatc aaaccagcca gacctttgat gtcattatct
     2701 ccgactgcac cgatcctatc ggtcccggcg aaagcctttt cacctcagca ttttatgaag
     2761 gctgcaaacg ttgcctgaat cctggcggta tcttcgtcgc acaaaacggc gtctgctttt
     2821 tacagcagga agaagccatc gacagccatc gcaaactcag ccattacttc agcgacgttg
     2881 gcttttatca ggcggcgatc ccgacctatt acggcggtat catgactttt gcatgggcga
     2941 cagataacga cgccttacgc catctctcaa ccgaaattat tcaggcgcgt tttctcgctt
     3001 ctggcctgaa atgccgttat tacaatccgg caatccatac ggcagctttt gccttacctc
     3061 agtatctgca agacgcactg gcttcacagc cgtcctaagg agaagataag aaattgaaaa
     3121 aactgaaact gcatggcttt aataatctga ccaaaagtct gagtttttgt atttacgata
     3181 tctgctacgc caaaactgcc gaagagcgcg acggttatat tgcttatatc gatgaactct
     3241 ataatgccaa ccgtctgacc gaaatcctgt cagaaacctg ttccattatc ggggccaata
     3301 ttcttaacat cgcccgtcag gattacgaac cacagggtgc cagcgtcact attctggtga
     3361 gtgaagaacc ggtcgacccg aaacttatcg acaaaacaga acaccccggc ccactgccag
     3421 aaacggtcgt tgcccatctc gataaaagtc atatttgcgt acatacctac ccggaaagtc
     3481 atcctgaagg cggtttatgt accttccgcg ccgatattga agtctctacc tgcggcgtga
     3541 tttctccgct gaaggcgctg aattacctga tccaccagct tgagtccgat atcgtaacca
     3601 ttgattatcg cgtgcgcggt tttactcgcg acattaacgg tatgaaacac tttatcgacc
     3661 atgagattaa ttcgattcag aactttatgt ctgacgatat gaaggcgctg tatgacatgg
     3721 tggatgtaaa tgtctatcag gaaaatatct tccataccaa gatgttgctt aaagagttcg
     3781 accttaagca ctacatgttc cacaccaaac cggaagattt aaccgacagc gagcgccagg
     3841 aaattaccgc tgcgctgtgg aaagaaatgc gcgagattta ttacgggcgc aatatgccag
     3901 ctgtttaacg tctcgggcgg agctcccagg ctccgccaga tttatttact tctgctgcac
     3961 gaaattgcgg taagccgcca cgacctgcag aaaatcctca acgccgcata gcgacaggct
     4021 ttcttcgtcg tagtagttca tcccttcttc catttcatcg ccagcgaatt ccagttgatt
     4081 ggcgcgaacc atcacctctt caccgtccat ccacagggtg tattcatgcc ctgcccgctg
     4141 ccaggaacgt tcgctacctt tcagtgcgtg cgccgcttgt tccacttcat caagcaaggc
     4201 caggttttct ttcacctctt cattaaacca gtgcccgacc acttcatgcc ccatggacat
     4261 acgcaccttt acaactccgg taatatcgcg cagaaattcg taatccatag tgtgttcctc
     4321 tgctcccggc aatgcgccga aatcgcattg cgccattgat gtaattatcg cagccttacc
     4381 ggagaaaaac agcggaacgg cgatggttta gaaataaaaa aatgcccggg aatctcattc
     4441 accgggcatt gtgtcgttta tgcgcagcgc gtgcgcttac ttattacacc gcagtctgga
     4501 aaatcacccc atcggctttt tcggtgtact gagaaagctg gttgaagttc agataacgat
     4561 aagtatcaac ggcagtttta tctacttgcg ccacgtaggt ctggtactct tccggcgtcg
     4621 gcagtttgcc aatcagcgcc gcaacagccg ccagttccgc agaagccagg aagacattcg
     4681 cgccagtacc cagacggttc gggaagttac gggtagaggt ggaaaccacc gtcgcaccgt
     4741 ctgctacacg cgcctggtta cccatacaca gggaacagcc agggatctcg atacgcgcac
     4801 cactcttacc gaagacgctg tagtagcctt cttcagtcaa ctgcgcggcg tccatacggg
     4861 ttggcggtgc cacccacagg cgggtcggca gttggccttt gtgcgcatcc agcagtttac
     4921 ccgcagcacg gaagtgaccg atgttggtca tgcaggaacc gataaatact tcgtcgatct
     4981 tctcaccctg taccgcagac agcggacgcg cgtcatccgg gtcgttcggt gcacagagga
     5041 ttggctcttt aatatccgcc agatcgatgt cgatcactgc cgcgtattca gcatctgcat
     5101 cggcttccag cagctcagga ttcgccagcc atttttccat gccctgaata cgacgttcca
     5161 gggtgcgacg atcgccgtaa ccttccgcga tcatccactt cagcaggacg atgttagagt
     5221 tcaggtattc gatgatcggt tctttgttca gcttgatggt acaaccagcg gcagaacgct
     5281 cggcggacgc atcggtcagt tcaaatgcct gctcaacttt cagatccggc agaccttcaa
     5341 tttccaggat gcggccagag aagatgtttt tcttgccttt cttctcaacg gtcagcagac
     5401 cttgtttgat cgcatacagc ggaatggcat gtaccagatc gcgcagggtg atgcccggct
     5461 gcattttgcc tttgaagcgc accagaacgg attccggcat atccagcggc attacgcctg
     5521 ttgcggctgc aaatgccacc agaccagaac ccgccgggaa agagataccg atcgggaaac
     5581 gggtatggga atcaccaccg gtaccgacgg tatccggcag cagcatacgg ttcagccagg
     5641 agtgaatgac gccgtcaccc ggacgcagcg acacaccgcc acggttcata atgaagtccg
     5701 gcagcgtgtg gtgcgtgttc acgtcaactg gcttcggata cgccgcggtg tggcagaaag
     5761 actgcatcac caggtcagcc gagaagccca ggcacgccag gtctttcagt tcatcacggg
     5821 tcatcgggcc ggtggtgtcc tgggaaccta cagaagtcat tttcggttca cagtacgcgc
     5881 ccggacgaat gcctttcacg ccacaggcac ggcctaccat tttttgcgcc agcgagaagc
     5941 cgcgatcgct ctcagcgaca tctttcgcct gacggaacac atcactgtgc ggcagaccaa
     6001 gtgcttcacg cgctttggtg gtcaggccac gcccgataat cagcggaata cggccaccag
     6061 cacgcacttc atcaatcagc acgtcggttt tcagttcgaa ggtcgccagc agttcgccgg
     6121 tttcgtggtt acgcacttca cctttgtacg ggtaaacgtc aatcacgtcg cccatgttca
     6181 ggttagagac gtcgacttcg attggcagtg cacccgcgtc ttccatcgtg ttaaagaaga
     6241 tgggtgcaat tttaccgccg aggcacaaac caccgccgcg tttgttcggc acatgtggaa
     6301 tatcatcgcc cataaaccac agaacggagt tagtggcgga tttacgcgaa gaacccgtac
     6361 ccacaacgtc accgacgtac gccagcggga aacctttctg ttgcagagct tcgatttgct
     6421 tgatcggacc aacaacacca ggctggtctg gctcaatacc ttcacgggcg tttttcagca
     6481 tcgccagcgc gtgcagtggg atatccgggc gtgaccacgc atccggtgcc ggagaaaggt
     6541 catcggtgtt agtttcgcca gtgactttga agacggtaac ggtcagtttt tcagccagcg
     6601 ccgggcgatt caggaaccat tcggcatccg cccaggactg cataacctgc ttcgcatatt
     6661 cgttgcctgc tttcgctttc tcttctacgt catagaagtt atcgaacatc agcagcgtgt
     6721 gagaaagtgc tttggcagca ataggtgcca gtttggcatc atccagcgcg tcgatcagcg
     6781 gatgaatgtt gtaaccaccc tgcatggtgc ccagcagttc gatggctttt tccggagtca
     6841 gcagagggga tttggcttcg cctttcgcga tagcagccag gaagcctgct ttgacatagg
     6901 cggcttcatc gacgcctggg ggaacacggt tggttaacag atctaacagg aattcttctt
     6961 cgcccgcggg cgggtttttc agcagctcta caagtgcggc catttggttt gcatccaggg
     7021 gtttgggcgc aatcccctca gcggcacgct cagctacgtg cttacggtat tcttctagca
     7081 cgacggttct cctcgctctc attgtcatag tgcggcaggc gattctcttc acgctcctgt
     7141 gagacagcag tttgtagggt aaatgcccag taccgcaacg ggcagaatag caggattttg
     7201 acgatgtgtt aatctgttta caaaaaagca acataaaaaa gtggctgaat cgttaaggat
     7261 ggtctagaga ttgtttccct ccgtaaaaac ttcgttaatt cgcatggtga taatcacctt
     7321 tctcaacgaa tcccaagatt agtcaaaatt taaactaccg cctctttata ctcggattca
     7381 cagcacctgc gggtggcagt tcgccgacca ttgcgatttc cttgagatcc gaattatgaa
     7441 aatgactttg ccgtttaaac cccatgtgct ggcgctaatt tgcagtgccg ggctttgtgc
     7501 cgcctctacc gggctatata taaaaagccg cacagtggaa gcgcctgtgg aaccgcaatc
     7561 gacacaacag actgcgcctg acatcaccgc agttacgctt cctgcaacgg tttccgcacc
     7621 gcccgtaacg cccgccgtcg tcaaatccgc attcagcact gcacaaatcg atcaatgggt
     7681 tgcgcctgtc gcgctgtacc ccgattctct gctttcacaa gtgttaatgg catcaaccta
     7741 tccggcaaac gttgctcaag cagtgcaatg gtcgcacgat aatccactta aacaaggcga
     7801 tgctgctatt caggcggtat ctgaccagcc gtgggacgcc agcgttaaat cactggtggc
     7861 ctttccacaa ttgatggcat tgatgggcga aaacccgcaa tgggtgcaaa acctgggcga
     7921 tgcttttctg gcgcagccgc aggacgtgat ggactcggta cagcgattgc ggcaactggc
     7981 gcaacaaacc ggctcgctga agtcatcaac cgaacagaaa gttattacca caacgaagaa
     8041 agctgtaccg gtaacacaga cagtcacggc tcccgtcata ccatccaata ccgtttcaac
     8101 tgccagcccc gtcattacag agcctgcaac aaccgtcatt tccattgagc ccgccaatcc
     8161 tgatgtggtc tatattccca actacaaccc aaccgtggtt tacgggaact gggccaatac
     8221 tgcgtatccg ccggtttatc tgccaccacc agccggagaa ccgtttattg acagctttgt
     8281 gcgcggattc ggctatagca tgggtgttgc taccacgtac gcactattca gcagcatcga
     8341 ctgggacgac gacgatcatg accatcatca tcatgacgat gatgattatc atcaccacga
     8401 tggcggtcat cgtgacggta atggctggca acacaacggc gacaacatca atatcgacgt
     8461 caacaatttc aaccgtatca ccggtgagca tcttactgat aagaatatgg catggcggca
     8521 caatccaaac taccgtaatg gtgtgcccta tcatgatcag gatatggcaa agcggtttca
     8581 tcaaaccgat gtcaacggcg gaatgagcgc cacgcagcta cctgctccaa cacgcgacag
     8641 ccagcgtcag gcggcagcaa gtcagtttca gcaacgaaca cacgccgccc ccgtcattac
     8701 acgagatacc caacgtcagg cagcggcaca gcggtttaat gaagctgaaa actatgggag
     8761 ctatgacgac ttccgcgact tcagccgtcg ccaaccactg acccagcaac aaaaggacgc
     8821 cgctcgtcag cgttatcagt cggcctcgcc tgagcagcgc caggcagtcc gcgagaaaat
     8881 gcagactaac ccacagatcc agcagcgaag agacgcagcg cgtgagcgta ttcagtccgc
     8941 ctcgcctgag cagcgccagg cagtccgcga gaaaatgcag actaacccac agatccggca
     9001 gcgaagagac gcagcgcgtg agcgtattca gtcagcctcg cctgagcagc gccaggtgtt
     9061 taaggaaaaa gtacagcagc gcccactgaa ccaacagcaa cgtgataacg cccgccagcg
     9121 tgttcaatca gcatcacctg aacaacgtca ggtttttcgg gaaaaagttc aggagagccg
     9181 cccacaacgt ctaaacgaca gtaaccatac tgtcaggctg aataacgagc aacggtcagc
     9241 agtacgcgaa cgtctctctg agcgcggagc aaggcgacag gaaaggtaaa ttgcagacgt
     9301 aaaaaaagcg gcgtggttag ccgctttttt aattgccgga tgttccggca aacgaacaat
     9361 tacttcttct tcgctttcgg gttcggcagg tcggtaatgc taccttcgaa cacttctgcc
     9421 gccaggccca cagactcgtg cagagtcggg tgcgcgtgga tggtcagtgc gatgtcttca
     9481 gcatcacaac ccatttcgat tgccaggccg atttcaccca gcagctcgcc gccgttggta
     9541 ccgacaatcg caccaccgat cacacggtga gattctttgt cgaaaatcag cttggtcata
     9601 ccgtctgcgc agtcggaagc gatagcacga ccagaagcag cccacgggaa ggtggcggtt
     9661 tcatagctga tgcctttctc tttcgcttct ttctcagtca gacctaccca tgcaacttct
     9721 ggttcggtat aggcgatgga cgggataact ttcggatcga agtagtgttt cttaccggcg
     9781 ataacttcag cggcaacgtg accttcgtga acacctttgt gtgccagcat cggctgaccg
     9841 acgatatcgc cgatagcaaa gatgtgcggt acgttggtac gcagctgttt gtcaacgcgg
     9901 atgaaaccac ggtcgtccac ttccacgcca gctttgcctg cgtcgaggtt tttaccgttc
     9961 ggcacacgac caatcgctac cagcacggcg tcgtaacgct gcggttcagc gggtgctttt
    10021 ttgccttcca tcgtcacata aataccgtct tctttcgctt caacggcggt aactttggtt
    10081 tccagcatca ggttgaattt cttgctgata cgcttggtga agactttaac gatgtctttg
    10141 tcagctgccg ggataacctg gtcgaacatt tcaaccacgt caatctgtga acccagcgcg
    10201 tggtatacgg tgcccatttc cagaccgatg ataccgccac ccattaccag caggcgttct
    10261 ggtacttctt tcagttccag cgcgtcagtg gagtcccaga tacgcggatc ttcatgcgga
    10321 ataaacggca gttggatcgg gcgagaaccc gctgcaatga tcgcgttgtc gaagttgatc
    10381 acggttttac cgttctcacc ttcaacttcc agggtgttag ccccggtaaa tttacccaga
    10441 ccgttgacca ctttgacttt gcggcctttc gccataccag ccagaccacc ggtcagctga
    10501 ttgatcactt tctctttcca ggtacgaatc ttgtcaatat cggttttcgg ttcgccgaag
    10561 acgataccgt gttcagccag cgctttggct tcttcgataa cttttgctac gtgcagcagt
    10621 gctttagaag ggatacagcc gacgttcagg caaacaccgc caagggtgtt gtaacgttct
    10681 acgattacgg tttccagacc taaatcagcg caacggaagg cagcggagta acctgcgggg
    10741 cctgccccaa gtaccacgac ctgagttttg atttcagtac tcatcatgac ctctatatat
    10801 ttatctccgg cggtcatacc cgtcgtcttt caggccgcag cggcgttagc ttcacgccct
    10861 caccccagtc acttacttat gtaagctcct ggggattctg gcgcttgcta ccttgctgca
    10921 cccagaaatc catagggtat gtgaagtaat accctaacca ccaccgggtc gttctatccg
    10981 tcggtatttt acaaaattgt taacaatttt taaacaacaa acggcaaccg atttgtctat
    11041 tcgctaataa cctcaataca ttcatgagat taccagaaaa aagccggccg ttgggccggc
    11101 tcttttactt acatcaccag acggcgaatg tcagacagcg tgttgttaat gatggtaatg
    11161 aaacgggcac catcagcacc gtcgatcacg cggtggtcga aggagagaga aatcggcagc
    11221 atcagacgcg gcacgaactc tttaccattc cacaccggct ccatcgcgga cttggaaacg
    11281 ccgaggatag ccacttccgg cgcgttcaca atcggcgcga agtgggtagt acccaggccg
    11341 ccgatgctgg agatggtgaa gcaaccgccc tgcatttcgc ccgcagtcag cttaccgtca
    11401 cgcgctttct tagaaatagt catcagctcg cgagacagct cgatgatgcc tttcttgttg
    11461 acgtctttga ataccggaac aaccagaccg ttcggggtat ccaccgccac accgatgttg
    11521 atgtatttct tcagggtcag acgctgaccg tcttccgaca acgaactgtt gaagcgaggc
    11581 atctgctcaa gagctgcagc aacggctttc atgatgaaga caaccggggt gatcttcaca
    11641 tccagcttac gtttcgccgc ttcttcgttc tgctgtttac ggaacgcttc cagctcggtg
    11701 atatcggttt tgtcgaagtg agtaacatgc gggatcatca cccagttacg gctcaggttc
    11761 gcaccagaaa ttttctggat acggcccagt tccacttctt cgatttcacc aaacttgctg
    11821 aagtccacct tcggccacgg cagcatgcct gggataccgc cgccagtcgc cgccggagct
    11881 gcttctgcac gtttgatagc ttctttcacg taagcctgaa cgtcttcgcg caggatacga
    11941 cctttacggc cagtgccctt cactttcgcc aggttaacgc caaactcgcg tgccagacgg
    12001 cggatcagcg gagtcgcgtg aacgtaagcg tcgttttctg caaattcaga tttgccttcc
    12061 gctttcgcag ccggtgctgc tgccggagct tcagcttttg ctgccggagc cggcgctgcc
    12121 gcttcctgtt tcgcaggagc tgccgcaggc gctgcgcctt caacttcgaa gatcataatc
    12181 agcgagccag ttttcacttt atcgccaacg ttgactttca gttccttcac gacgcctgca
    12241 aacggagccg gaacttccat agaagctttg tcgccttcta cggtaatcag tgactgttca
    12301 gcggcaactt tgtcgcccac tttcaccatc acttcggtca cttcaacttc gtcactgccg
    12361 atatccggaa cgttaacttc tttcacgcca gcagctggtg caggggccgc tgccggagcc
    12421 gcttcctgtt tagccgccgg agctgccgcg cctgcttcac ccgcgacttc gaagaccata
    12481 atcagcgagc cggtagacac tttgtcaccc acgttcactt tgatctcttt cacggtgcca
    12541 gcaaacggag ccggaacttc catagacgct ttatcgcctt ctacggtgat cagcgactgt
    12601 tcagcttcaa ctttatcgcc aactttcacc aggatttcgg tcacttcaac ttcgtcgctg
    12661 ccgatatccg gaacgttaac gtcttttgcc gctgcagccg ctggtgctgc tgccggagct
    12721 gcttctttct tctcttctgc ctgagcaggt gcagcgtctg ctgcaccgtc ggcggaatcg
    12781 aaaatcataa tcagtgcgcc ggtctgggtt ttatcgccaa cagagacttt gatctcttta
    12841 acgatacccg cctgcggaga cggaacttcc atagaggctt tgtcgccttc tacggtgatc
    12901 agcgactgtt cggcttcaac tttgtcgccc actttgacca ggatctcggt gatttcaact
    12961 tcatcagccc cgatgtccgg tactttgatt tcgatagcca ttattctttt acctcttacg
    13021 ccagacgcgg gttaacttta tctgcatcga tgttgaattt ggcgattgcg tcagcaacca
    13081 ctttcttatc gatttcgcca cgtttagcca gttcgcccag cgccgctaca accacgtagg
    13141 aagcatcaac ttcgaagtgg tgacgcaggt tctcacggct gtcggaacga ccgaagccat
    13201 cagtacccag tacgcggtag tcgtcagccg gtacgtaagt acggacctgc tcagcgaaca
    13261 gtttcatata gtcggtagat gccactgccg gagcgtcgtt catcacctga gcgatatacg
    13321 gaacgcgcgg agtttccagc gggtgcagca tgttccagcg ttcacaatcc tgaccatctc
    13381 gcgccagttc ggtgaaggag gtcacgctat aaacgtcaga acctacgccg taatctttcg
    13441 ccaggatctc tgccgcttca cggacgtgac gcaggataga accggagccc agcagctgaa
    13501 ctttaccttt gctaccttca atggtttcga gtttgtagat acctttacgg ataccttcct
    13561 cagcaccttc cggcattgcc ggcatgtggt agttttcgtt cagcgtggtg atgtagtagt
    13621 aaacgttctc ttgtttttca ccgtacatac gctccagacc gtcatgcata atgacagcaa
    13681 cttcgtaagc gtaagccggg tcgtaagaga tacagttcgg aatagtcagc gactgaatgt
    13741 ggctgtgacc atcttcgtgc tgcagacctt cgccgttcag ggtggtacga ccggaagtac
    13801 cgccgatcag gaagccacgc gcttgctggt cgccagccgc ccagcacaga tcgccaatac
    13861 gctggaagcc gaacatcgag taatagatgt agaacgggat catcggcaga ttgttggtgc
    13921 tgtaagaggt cgccgctgcc agccaggaac aacctgcgcc cagctcgttg atcccttcct
    13981 gcagaatctg acctttctcg tcttctttat agtaagcaac ctgctcgcgg tcctgcgggg
    14041 tgtactgctg accgttcggg ctgtaaatac caatctgacg gaacagacct tccataccga
    14101 aagtacgcgc ttcgtcggcg atgatcggta ccagacgatc tttgatcgac ttgttcttca
    14161 gcattacgtt cagggcacga acgaaagcga tagtggtaga gatctctttg ctctgctctt
    14221 ccagcagcgc gccgaagtct tgcaggctcg gcagctcaag cttctcggtg aagttcggct
    14281 gacggcttgg cagataaccg tgcagtttct gacgctgtgc gtgcagatag gtatgctctt
    14341 cagaaccttc cgggaaggtg atgtacggca gtttttcgat atctgcatca gacaccggca
    14401 cattgaaacg gtcgcggatg tgacgcacgc cgtccatgtt cattttctta acctggtgcg
    14461 cgatgttttt accttcagcc gcgtcgccca tgccgtaacc tttaatggta tgagcaagga
    14521 ttactgtcgc tttgcctttg gtttcctgcg ctttcttgaa tgcagcgtag attttcttcg
    14581 gatcgtgacc accacggttc agtgcccaga tctgctcgtc agtccagtct gcaaccagtg
    14641 ctgcggtttc aggatattta ccgaagaagt gttcacgaac gtacgcacca tctttcgatt
    14701 tgaaggtctg gtagtcgccg tcaacggttt cgttcatcag ctggatcagt ttaccgctgg
    14761 tatctttacg cagcagttca tcccaacggc taccccacat cactttgatc acgttccagc
    14821 cagcaccttc gaagatgcct tccagttcgt tgatgatctt gccgttaccg gtgaccgggc
    14881 cgtcaagacg ctgcaggtta cagttgataa cgaagaccag gttatccagt ttttcacggg
    14941 tagcgatggt gatcgcacct ttggattccg gttcgtccat ttcaccgtcg ccgaggaacg
    15001 cgtaaacggt ctgtttagag gtatctttca ggccacggtg ttccagatat ttcaggaatt
    15061 tagcctggta aatagcacca atcggcccca gacccataga tacggtcggg aactgccaga
    15121 attccggcat cagtttcggg tgcggatagg aagagaggcc attgccgtga acttcctgac
    15181 ggaagttatc cagctgctcc tgagtcagac gaccttccag gaaagcacga gcgtacacgc
    15241 ccggggagat gtggccctgg aagtaaacca ggtcgccgcc atcctgctcg ttgcgtgcac
    15301 ggaagaagtg gttaaagcac acatcataaa tggttgcgga agactggaag gacgccatat
    15361 ggccgcccag ttcgaggtct tttttcgacg cacgcagcac cgtcatgatg gcgttccagc
    15421 ggatagctga acgaatacgg cgttccagtt ccagattacc cggatactcc ggttgttctt
    15481 caacggggat ggtgttgatg tagttgctga tacctgtgcc tgcggctacg tttacaccgc
    15541 ctttgcgggc ttcagcaagc agttggtcga tcagatactg agcacgctca acaccttctt
    15601 cacggatgac cgattcgatc gcctggagcc agtcgcgagt ttcgatcgga tccacgtcat
    15661 ttgggaaacg ttctgacatg ggttattcct tatctatcta ataacgttga gttttctgga
    15721 acctgtccca ttgaactctc gccggaaagc tcaataagac aggttctacg tttagttgcc
    15781 gcgcttttat atgcgcttga tttacaacat cttctggata atttttacca gaaaaatcac
    15841 taattctttc gttgctccag acgacgcaga gaacgctcac ggcggctctc ttcacgactt
    15901 ctgtcgagca aaatttcttc gataaaggcc agatggcgat gcgatgcttc gcgcgcttct
    15961 tccggcttac cggccataat cgcttcaaat atgcgggtgc ggtgactact caccagcggc
    16021 agcatctcgc gacgcgaata gagcaattcg aagttctggc ggacattctg ggccaacatc
    16081 ggctccatac accttagcag atgaagcaga accacattgt gggccgcttc agtgacagca
    16141 atctgatact ggagtacggc gtttgattcc gcgtccagat cgccagactg ctgcgccagc
    16201 tctatggcgt ggtggagttc acggatgcgt tccttgtctt catcggtact acgcagcgcg
    16261 gcgtaataag cggcgatacc ttccagggcg tgtcgtgttt cgagcaagtc atactgtgac
    16321 tcaggatggt cggagagcag ctccaccagc ggatcgctga agctttgcca taggctgctc
    16381 tggacaaaag tgccgccacc ctggcgacga agcaacaagc ccttcgcttc gagacgttga
    16441 atcgcctcac gcaaggaggg acgggagacg tcaaactgtt ttgccagttc gcgttccggt
    16501 gggagttttt cgcccgggcg gagagtgcct tcgaggatca aaaactccag ttgctgctca
    16561 atcacatcgg agagttttgg ttggcggatt ttgctgtagg ccatgagttc ctgtcttaag
    16621 ccacttgccg aagtcaattg gtcttaccaa tttcatgtct gtgacgctaa agtaacaaag
    16681 tattcacctt atgtccatac aggttttgat tgaaatcatg aaactgtgca cattttaaca
    16741 acttgacata tataacgttt caaagttgta actatgcaca aatgtagact ttacgtagga
    16801 aaggagtttt ttaacgatta ttaaactata aaaatccgaa ttgaaccgat tcacttacca
    16861 attttgtgat ttttaattca attaaaacga atttaaattc attctacata ttgagagggg
    16921 ttgaggctga gctttacaaa cggtttcttt ttaagcaact catcttcaac catgcataaa
    16981 gcgggtgcat tcgctgccgc ataccattat tcttgatctg acggaagtct ttttgtaaca
    17041 attcaaactt ctttgatgta aacaaattaa tacaacaaac ggaattgcaa acttacacac
    17101 gcatcactgc gtagatcaaa aaaacaacca ccgcacgagg tttcatgatg gaaggtcaac
    17161 agcacggcga gcagctaaag cgcggcctta aaaaccgcca tattcagctt atcgcgctgg
    17221 gtggcgcgat agggaccggg ttattcctgg gtagcgcctc cgtaatacag tccgcagggc
    17281 cagggattat cctgggttac gccattgctg gttttatcgc ctttctgatc atgcgtcagc
    17341 tgggtgaaat ggtggtcgaa gaacctgtcg caggctcctt tagccacttt gcttataaat
    17401 actggggcag ttttgccggt ttcgcctctg gctggaacta ctgggtactg tacgttttag
    17461 ttgccatggc tgagctgact gccgtgggta aatacattca gttctggtat ccggaaatcc
    17521 ccacctgggt ttctgccgcc gtattctttg tggtgattaa cgccatcaac ctgaccaacg
    17581 ttaaagtgtt tggcgagatg gagttctggt ttgccattat caaagttatc gcggtggtag
    17641 cgatgatcat cttcggcggc tggctgctat tcagtggcaa cggcggcccg caggcgaccg
    17701 ttagcaacct gtgggatcag ggtggtttcc tgccgcacgg cttcaccggg ctggtgatga
    17761 tgatggcgat tatcatgttc tcgttcggtg gtctggaact ggtggggatc accgcagcag
    17821 aagctgataa cccggagcaa agtataccga aagcaactaa ccaggttatc taccgcatcc
    17881 tgattttcta tattggttcg ttagccgttc tgctctcact gatgccgtgg acccgcgtta
    17941 ccgccgatac cagtccgttt gtgctgatct tccacgagtt aggcgatacc tttgtggcga
    18001 atgcgctgaa catcgtggta ctgactgcgg cgctctccgt gtacaacagc tgcgtatatt
    18061 gcaacagccg tatgctgttt ggtctggcac aacagggtaa tgcgccaaaa gcgctggcgt
    18121 ctgtcgataa acgtggtgta ccagtaaata ccattctggt gtctgcactg gtaacggcgt
    18181 tgtgcgtact gattaactac cttgccccag agtccgcttt cggactgtta atggcgctgg
    18241 tggtatctgc actggtaatc aactgggcga tgattagcct ggcgcatatg aaattccgtc
    18301 gcgccaagca ggaacaaggc gtggtaactc gcttccctgc tctgctttat ccgctgggta
    18361 actggatctg cctgctgttt atggcggcgg tactggtgat tatgctgatg accccaggaa
    18421 tggcgatttc ggtatacctg atcccggtat ggctgatcgt gttaggtatc ggctatctgt
    18481 ttaaagagaa aaccgccaaa gccgtaaaag cgcattaatc tctctacgcc ctcacccgta
    18541 cagggtgagg gcaataatct ttacaccaac gccccgtaaa tcgtcagtag tgcaatcacc
    18601 accacgacca cgaacgaggt tttcttcgcc attgaaaccg ctgccttcgg cgtctccacc
    18661 ttatcgacat gcggttcacg cgccagagag aactgcgcca gacgcgttaa cacctgatac
    18721 tgcgaagtat ggaaatcacc cagcgaagca aaccaggccg gtaacgcttt ctcaccatga
    18781 ccgatcaagg catataccac acccgcaaga cgaaccggca cccaatccag tacatgaagc
    18841 acggcatcaa tgccggactg taaacgatga tgcggcgtct gatatcgcgc cagccagtat
    18901 tgccatgcac gcaaaaacgc ataccccatc agcgtaacgg gtccccaggt tccccccaca
    18961 atcagccaga acagcggtgc aagataaaaa cgaaagttaa tccacagcaa tgcattttgc
    19021 agctcacgca aatactcacg ttcgtcgcag cctgccggga cgccgtgaat catggtgagt
    19081 tcgccagcca tcgtggcacg ggcatggcta tcattacgtg aagcagctgt cagataagca
    19141 tgataatgaa gacgaacttt acctgcgcca atacacagca aaccaatcag cagccacacc
    19201 agtagcgtgg gaacgttgaa caatactccc tgcaatgcgc gtaacagtaa aaaagtcacg
    19261 cccatcgcaa taatggtcat gcctaacgtg cgcccgagag aaaaatgttt cacccgccga
    19321 aagaacgctt caagacgatg atcaagctgc cagtgctcgc ccaacttaaa caggcgctcg
    19381 aaaattaaca ccagtaaggt tgtaaatagc gtcatgttgt ctccttgctg accagcacac
    19441 gaaaccgtgc ccaatcaaat gcaggaccgg gatcggtttt ccgatccggc gcaatatcac
    19501 aatggcccgt catgttttta gcgatatccg gatagcaatc aatcagtgcc cgcgtaaccg
    19561 ccgcaagctg ttgatactgc gcatcggtat acgccagcgt atcggtgcct tcaagctcaa
    19621 tcccaataga aaaatcattg cagcgttcgc gcccctgata ctgagagact cccgcatgcc
    19681 atgcacgttt atcgaaagga acatactgga ctatttcacc atcacggcga atcaaacagt
    19741 gagcggagac gcgcaaatgg gcgatctcag caaagaaagg atgtgcctgc ggatcaatag
    19801 ttccagtgaa taatgcgtcg atccacggac cgccaaactc gcctggcggc aggctaatat
    19861 tgtgcaccac cagcagggtg ggtgtttcgt catccgggcg gcaatcgtaa tgtggtgagg
    19921 gaacgcggcg cgcgccaacc agccacccct gttctaacaa catgcaggat ctccttatat
    19981 gtggtgctaa tacccggttc agagtagcat gtttctacct tatgattcgt tagctatctg
    20041 gagttttaac atgccgcctc gccgctataa ccctgacacc cgacgtgacg agctgctgga
    20101 acgcattaat ctcgatatcc ccggcgcggt ggcccaggcg ctgcgggaag atttaggcgg
    20161 aacagtcgat gccaacaatg atattacggc aaaactttta ccggaaaatt ctcgctctca
    20221 tgccacggtg atcacccgcg agaatggcgt cttttgcggc aaacgctggg ttgaagaggt
    20281 gtttattcaa ctggcaggcg acgatgtcac cataatctgg catgtggatg acggcgatgt
    20341 catcaatgcc aatcaatcct tgttcgaact tgaaggccca tcccgcgtgc tgttaacggg
    20401 cgaacgcact gcgcttaatt ttgtgcaaac cctttcagga gttgccagta aggtacgcca
    20461 ctatgtcgaa ttgctggaag gcaccaacac gcagttgttg gatacgcgca aaaccttacc
    20521 cggcctgcgt tcagctctga aatacgcggt actttgcggc ggcggagcga atcaccgtct
    20581 ggggctttct gatgccttcc tgatcaaaga aaaccatatt attgcctccg gctcagtgcg
    20641 ccaggcggtc gaaaaagcgt cctggctgca cccggatgcg ccagtagaag tcgaagtaga
    20701 gaatctggaa gaacttgatg aagccctgaa agcaggagcc gatatcatca tgctggataa
    20761 cttcgaaaca gaacagatgc gcgaagccgt caaacgcacc aacggcaagg cgctactgga
    20821 agtgtctggc aacgtcactg acaaaacact gcgtgaattt gccgaaacgg gcgtggactt
    20881 tatctccgtc ggtgcgctaa ctaaacacgt acaagcactc gacctttcaa tgcgttttcg
    20941 ctaatagttg acagatttat ccgctccatc gcgggcggat aatctcccct tccccaactt
    21001 tcttcgtaac gcctcgcaaa tttcttgtct ttcgctgcaa cgaaataaaa aacctcggaa
    21061 tctgctttcc cgtttcgttt tttgcccctc gcctgtgctg atggcgtttg ccaaagtagc
    21121 gccaaccaaa tcaaggagcg aaacagatgg acaagcaacg cggttttaca cttatcgaac
    21181 tgatggtggt tattggcatc attgccattt taagcgccat tggtattccc gcttatcaaa
    21241 actacctgcg caaagccgca ctcaccgaca tgctacaaac ctttgtgcct taccgtaccg
    21301 ccgtagagtt gtgcgcgctg gaacatggtg gattagatac ctgcgacggt ggcagcaatg
    21361 gcattccctc gcctaccacc acccactatg tttcagccat gagtgtggca aaaggcgtgg
    21421 tgtcgctgac cgggcaagaa agtctcaatg gactaagcgt cgtcatgaca ccgggttggg
    21481 ataacgcaaa cggcgtcacc ggctggacgc gcaactgcaa tattcaaagt gacagcgcat
    21541 tgcatcaagc ctgcgaagat gtcttccgct ttgatgacgc caactaagga gcggcaatga
    21601 atattccaca gctcactgcc ctgtgtctgc gttatcatgg agtcttgctg gatgccagcg
    21661 aagaggtggt tcatgttgcg gtagtcgatg caccttcgca tgagctactg gacgcattgc
    21721 atttcgctac caccaaacgt attgagatca cctgctggac gcgccaacaa atggaaggtc
    21781 acgccagtcg cacacaacag acattgcccg tagctgttca ggagaagcat cagcccaaag
    21841 cagagttgct aactcgaacg ttacaatctg cgctggaaca acgcgcgtct gatattcata
    21901 tcgaaccagc ggacaatgcc taccgcatcc gcttgcgtat cgacggcgta ttgcatcctt
    21961 taccggatgt ttcaccggat gccggagtcg cattaaccgc cagattaaaa gtgctgggaa
    22021 acctggatat tgcggaacat cgcctgccgc aggacgggca attcactgtc gaactggcag
    22081 gaaacgccgt ctcatttcgt attgcgacct taccatgtcg gggtggtgaa aaggtggtat
    22141 taaggttgtt acagcaggtg agccaggcac tggatgtcaa cacgcttgga atgcagccgt
    22201 tacaactggc ggactttgct catgccttgc aacaaccaca gggactggtg ctggtaactg
    22261 gccctacagg cagcggcaaa acggtcacgc tttatagtgc cctgcaaacg ctgaataccg
    22321 ctgacattaa tatttgtagc gtcgaagatc cggttgagat ccccatagcc ggactaaacc
    22381 agacgcaaat ccatccgcgt gccggactca cctttcaggg cgtgttgcgt gcgttattgc
    22441 gccaggatcc tgacgtcatc atgatcggag agatccgcga tggcgaaaca gcagagatcg
    22501 ctattaaagc ggcgcaaact ggtcacctgg tgttgtctac cctacacact aattccacct
    22561 gcgaaacgct ggtacgttta cagcaaatgg gggtcgcccg ctggatgcta tcatcggcgc
    22621 ttacgctggt aatagcccag cgtctggtac gcaaactttg cccacattgt cgccggcagc
    22681 aaggggagcc catccacatt ccagacaatg tatggccatc gccgctgccc cactggcagg
    22741 cacccggttg tgtacattgc taccacggtt tttatggtcg tacggcctta tttgaagttc
    22801 tgcccataac gccggtcatt cgtcagctta tttccgctaa taccgacgtt gaatcgctgg
    22861 aaacgcacgc acgacaggcg ggtatgcgta cgctttttga aaacggctgc ctggccgtgg
    22921 agcaaggctt aaccaccttt gaagagttaa tccgcgtact ggggatgccg catggcgagt
    22981 aagcaactct ggcgctggca tggcataacc ggcgacggca atgcgcaaga tgggatgcta
    23041 tgggcagaga gccgtgcttt gctgctcatg gcactacagc aacagatggt taccccactt
    23101 agcctgaagc gaatcgccat caattctgcg cagtggcgag gagataaaag cgcggaagtc
    23161 attcatcaac tggcgacgct actcaaagcc gggttaacgc tttctgaagg gctggcactg
    23221 ctggcggaac agcatcccag taagcaatgg caagcgttgc tgcaatcgct ggcgcacgat
    23281 ctcgaacagg gcattgcttt ttccaatgcc ttattaccct ggtcagaggt atttccgcca
    23341 ctctatcagg caatgatccg cacgggtgaa ctgaccggta agctggatga atgctgcttt
    23401 gaactggcgc gtcagcaaaa agcccagcgt cagttgaccg acaaagtgaa atcagcgtta
    23461 cgttatccca tcatcatttt agcgatggca atcatggtgg ttgtggcaat gctgcatttt
    23521 gttctgccgg agtttgccgc tatctataag accttcaaca ccccactacc ggcactaacg
    23581 caggggatca tgacgctggc agactttagt ggcgaatgga gctggctgct ggtgttgttc
    23641 ggctttctgc tggcgatagc caataagttg ctgatgcgcc gaccgacctg gcttatagtg
    23701 cggcagaaat tgctgttacg catcccgatt atgggttcac tgatgcgggg acaaaaactc
    23761 acgcagattt ttacgattct ggcgctgaca caaagtgcag gcattacttt tttgcagggc
    23821 gtagagagcg tcagagaaac aatgcgctgc ccgtgctggg tgcaacttct gacacaaatc
    23881 cagcacgata tcagtaacgg tcatcccatc tggctggcgc taaaaaatac cggggagttt
    23941 agcccgctct gtttgcaatt agtgagaaca ggagaggcat ccggctcgct ggacctcatg
    24001 ttagacaacc tcgcccatca tcatcgggat aacacaatgg cgctggcgga taacctcgca
    24061 gccttactgg aaccggcgtt gctgatcata acgggaggaa ttatcggtac gctggtggtg
    24121 gcgatgtatc tgccaatttt ccatttaggc gatgcgatga gtgggatggg ataacgctgg
    24181 cgtgttgctc cacaccagcg ttggaagatt acaggttgtt gaagatgcgg ttttcttgtt
    24241 cctgcacacg gataaatgtg gtgcgcttgg tcagttcttt caggcgtgaa gccccaacgt
    24301 atgtacaagc tgaacgcagg ccgcccaaaa tatcacgcgc ggtattttca accgggcctc
    24361 gcagcggcag cttaacggtt ttaccttctg ctgcgcgata ttccgcaacg ccgccaacgt
    24421 gacgtttcat cgcagactcg gagctcatgc cgtagaacag cataaatttc tcgccgttct
    24481 cctcaacgat gcgaccgccg ctctcttcgt ggcccgccag catgccgcca agcatgacga
    24541 aatcggcacc gccgccaaag gcttttgcga catcgcccgg cgtggtgcag ccaccgtcgc
    24601 tgacgatcat tccgcccaga ccgtgcgccg catcggcaca ttcgattacc gcagaaagct
    24661 gcggataacc gacgcctgtt ttgacgcgag ttgtacaaac agaacctggg ccaatgccaa
    24721 ctttaacgat atcagcaccg gagaggataa gctcctcaca catttcacca gtcactacgt
    24781 tgccagcaca aatggttttg gtcggccacg cttcacgcgc tttcgcaacg aactgcacga
    24841 agtgttcgga ataaccattc gccacatcaa tacaaacgaa gtttaatgcc gggttcaagt
    24901 cgagaatctg tttagttttt tcgaaatccg catcagacgt accggtagaa accatcacat
    24961 gtttcagcac atcagcggaa gaattgttga taaacgcttg ccactcttcg acagaatagt
    25021 gtttatgcac agcagtcaaa atatcaaaag aagccagcgc agaggccata gaaaatgtac
    25081 ctacggtgtc catatttgcg gcgataattg gcacgccgga ccagctctga cctgaatgtt
    25141 tgaaggtgaa ttgacgttcc agttcaacat cggaacggct tttaagagtg gagcgtttag
    25201 ggcggatgag aacgtcttta aaacctaact tcagatcttc ttcaatacgc atgtgcggat
    25261 tcctggggtt aatggctata agggtaagag cgcaactcca gtgacgctat catacgcact
    25321 aatcaatgcg gcgcaagact gcgaaattgt ctttttttac gctacaatcc cataaattta
    25381 cctggtaaat agtgagcgaa cgtttcatca atttcgcttt cgtgcgctca tttttaacgt
    25441 tttgataatc atgatgaatt cccgggaata taaattatga ggtatatagt tgccttaacg
    25501 ggaggcattg gcagtggcaa gagtaccgtt gccaatgcgt ttgctgatct cggaattaac
    25561 gtcattgatg ccgatattat tgcgcgtcag gtggttgaac caggtgcacc tgcgctacat
    25621 gccattgctg atcactttgg cgctaacatg attgctgctg atggaacatt gcagcgccgg
    25681 gccttgcgcg agcggatctt cgccaacccg gaagagaaaa actggcttaa cgacctgctg
    25741 catccgctga ttcagcaaga gacgcaacac cagatccagc aagctacttc cccctatgta
    25801 ctgtgggttg tgccattgct ggtagaaaac tcactgtata aaaaagcgaa tcgagtgctg
    25861 gtggtggatg tcagcccaga aacgcaactt aagcgcacca tgcagcgcga tgatgtaact
    25921 cgcgagcatg tcgaacaaat ccttgctgct caggcaacgc gcgaagcccg ccttgccgtg
    25981 gcagatgacg tcattgataa taacggcgca ccggatgcta tcgcatcgga tgttgcccgc
    26041 ctgcacgcac actatttgca gcttgcgtcg cagtttgtct cacaggaaaa accgtaatgc
    26101 agacccaggt cctttttgaa catccactaa atgaaaaaat gcgtacatgg ctgcgcattg
    26161 agtttttgat tcagcaactc accgttaatt tacccatcgt tgaccacgct ggcgcgctgc
    26221 atttcttccg taatgtcagt gaattactgg atgttttcga gcgcggcgaa gtccgcactg
    26281 agctgttgaa agaacttgac cggcagcaac gtaaactcca gacctggatt ggcgtgcctg
    26341 gcgtggacca gagccgtatt gaagcattaa ttcagcagtt aaaagcggcg gggagcgtat
    26401 taatttccgc gccgcgtatc gggcaatttc tgcgtgaaga tcgtttgatt gctctggtgc
    26461 gtcagcgact gagcatccca ggcggctgtt gcagctttga tttacctaca ttgcacattt
    26521 ggctgcatct accccaggcg cagcgcgaca gccaggtaga aacctggatt gccagcctga
    26581 acccgctcac ccaggcactt accatggtgc tggatttaat tcgccagtcg gctcccttcc
    26641 gtaaacaaac cagcctgaat ggtttttatc aggataacgg tggcgatgcc gacttgctgc
    26701 gcctgaatct gtcgctcgat tcacagcttt atccgcaaat ttccggacat aagagccgtt
    26761 ttgccattcg ttttatgccg ctggacactg aaaacggaca ggtaccggaa cgtctggatt
    26821 tcgaactggc ctgttgctaa ggagtgaaaa tgtcagaaac tattacggtg aattgcccaa
    26881 cctgcgggaa aacggtggtg tggggtgaaa tcagcccgtt tcggccattt tgctccaaac
    26941 gttgtcagct gatcgacctc ggagaatggg ctgctgaaga aaaacgaatc cccagcagtg
    27001 gcgatctttc cgaaagcgat gactggagcg aagagccaaa gcagtgacat ttgtctgatg
    27061 ccgcacgtag gcctgataag acgcggacag cgtcgcatca ggcatcttgt gcaaatgtcg
    27121 gatgcggcgt gaacgcctta tccgacctac agacgcttaa gcttcgcaat taccggttca
    27181 ttggctggcg gaaaatcatc ggcattaaga ccgactagcg acatccactc accgggttgc
    27241 ccttctttac cccacggctc cccttcccag ctttcgacca gccagaacca cagagttata
    27301 tgcctgtccg ggaattcata ttccagtttt tcaaatagcg aaaaatgttg tggggtaatc
    27361 ccgacttctt cctgaagttc acgcaccacc gcctgttccg gcgtttcgcc catttcaatt
    27421 ttaccgccgg gaaactccag tttattcgcc atgtgcgcat ctgctgcgcg acgcgttata
    27481 aagatttcat tgttctcgtt gcgaataata cctaccgcaa tttgcagctt tttcattgtc
    27541 ttaaacctat aaaaaaggcg cagaatcctg cgccttttac ttcaactgtt agcttttatt
    27601 gcaggcgacc atggcactgc ttgtattttt taccagaacc gcacgggcaa ggatcgttac
    27661 gtcctacttt gcgctcaccg gtttgcgccg ccagtgcagc tgcggctgca gagtcgtcat
    27721 cctgatggct aagctgctgc atttgcgcta aacgctcggc ttccatacga cgctgttgtt
    27781 ccagctcctc aacctcttca ggcatacgta cctggacttt gctcagcgtg ctgataactt
    27841 catattttaa cgactccagc atcgctgcaa acatggagaa cgattcacgt ttgtattcct
    27901 gcttcggatc tttctgtgca tagccacgca ggtggatacc ctgacgcaga tagtccatcg
    27961 ccgccaggtg ctctttccac agagagtcga gagtttgcag catgacgcct ttttcgaagt
    28021 gacgcatcat ctcagcacca accacttctt ctttacgctg atacacttcg atggactgcg
    28081 ccagaattcg ctcacgcagc gtctcttcat gcagttctgg ttctttatcc agccactcgg
    28141 caattggcaa atcgaggtcg aaatcgttct tcagacgttc ctgcagcccc ggaatatccc
    28201 acatttcttc cagtgactgt ggcggaatgt aggcatcaat ggtcgctttg aacacatctt
    28261 cacgaatgct gttgatggtt tcgctcacat cgctgacatc cagcagttcg ttacgctggg
    28321 agtaaatggc gcgacgctga tcgttagcca cgtcatcata ttccagcagt tgcttacgaa
    28381 tgtcgaagtt acggctttca actttacgct gggcgttggc aatcgcttta gtcacccacg
    28441 ggtgttcaat ggcttcgcct ggcttcatac ccagtttacg catcatgccg gatactcggt
    28501 cggaagcaaa aatacgcatc agcgcatctt ccatcgacag gtagaaacgg gaagaaccag
    28561 catccccctg acgaccagaa cgaccgcgca actggttatc gatacgacgg gattcgtgac
    28621 gctcggtacc gatgatatgc aggccacctg cttccagtac cgcatcgtga cgtacctgcc
    28681 agtcggcttt aattttttca atttgctcta cggtcggatt ttccagcgcg gcaacttctg
    28741 cctgccagct accaccgagc acaatatctg taccacgacc cgccatattg gtcgcgatag
    28801 tcaccgcagc cggataacct gcctgagcaa caatcgccgc ttcgttggcg tggaatttgg
    28861 cgttcaggac gttgtgctta ataccggctt tggtcagttc gtttgacacc agctccgatt
    28921 tttcgatgga gatagtaccc accagcaccg gctggccttt cgcagtacgt tctttgatat
    28981 cttcaatgat cgcctgaatt ttttccgctt cagtcatgta gaccaggtcc ggcagatctt
    29041 tacgaatcat tggacggttg gtcggaacaa cgacggtatc cagcttgtag atggagctaa
    29101 attcgaaagc ttcggtatca gcagtaccgg tcatccccgc cagtttttca tacagacgga
    29161 agtagttctg gaaggtgatc gaagccagcg tttggttttc gttctggatc tgcacacctt
    29221 ctttcgcttc cacagcctgg tgcagaccat cggaccagcg acggccctgc atggtacgac
    29281 cggtgtgttc gtcaacgatg ataacttcac catctttaac gatgtagtcg acgtcgcggg
    29341 taaacagcgc atgagcgcgc agcgccgccg ttacgtggtg catcagcatg atgttggccg
    29401 gagagtacag agactcccct tcatccatga tgccctcttt caccagcagt tcttcaatca
    29461 gcaccagacc acgttcggtc aggttcacct ggcgagattt ttcgtccacc gagaagtggc
    29521 cttcgccctg gaaggtttcg gagtcttctt tttcctgacg gatcaggtgc ggaataattt
    29581 tattcacgcg tttatacatt tccgagctgt cttctgccgg gccggaaatg atcagcggtg
    29641 tacgcgcttc atcgatcagg atggagtcca cttcgtccac cagcgcatag tgcagtttac
    29701 gctgtacacg ttcttcaggg ctgaacgcca tattgtcgcg caagtagtca aagccgtatt
    29761 cgttgttcgt accgtaagtg atgtcagctg cgtaagcttc gcgctttgcc ggtgctggca
    29821 tgcccggcag gttgataccg acagtcaggc caaggaattc aaacagcgga cggttgtttt
    29881 cggcgtcacg ttgcgccagg tagtcgttga cggtaactac gtgcacgcct ttaccggtta
    29941 gtgcgttcag gtaagcaggc agcgttgcgg tcagggtttt accttcaccg gtacgcattt
    30001 cggcgatgca gcgttcgtta agaaccatac cgccgagtaa ctgaacgtcg aagtgacgca
    30061 taccaaagac gcgcttactt gcctcacgta ccacggcgaa agcttccggg atcagatttt
    30121 ccagcacttc gcctttttcc agacgtgcac gaaactctgc ggttttccct ttcagttctt
    30181 cgtcggagag tttttccatc tccggttcca tggcattgat gatgttgacc actttgcgca
    30241 tccggcgcag ggtgcgatcg ttacgactac cgaaaatttt agttaacaat ttgattagca
    30301 taataaaatc tcaaacgccc cgcgttgcgg agttaataaa atgaagtaaa ggtttattgt
    30361 tgttaggtga ggcgttgagg gccagcacgg atgccttgcg cctggcttat ccagacgggc
    30421 gtgctgaatt ttgcttgtgg ggtaaaatgc gcataatcaa tgcgataacc cttttcagac
    30481 ggcgtgcctt cctgggtcag cagcgcgctg agcgtatcca gtaatgcaag atgttgcgcc
    30541 tgaagaggca aagattcttc agcaacgggc agtgtttgcg gtgccattgc gaaagaaaga
    30601 tgacggatta ccgtgcgaat ggcatgttga tgccagtaat caacggaata gttcgaattc
    30661 gggcggcgtg tgttcgcttc cagcaaggcc aattgaccaa agttaacttt ggctgaaggc
    30721 tcgtggttgc gggttgtcgc ttttgcgggc gcgtttggtt cggcggcgtt gctgagcgca
    30781 ggcaaaccta aactcgccgc aaccatccct aataagagat gcggccagaa gtagcgttta
    30841 ccaaactgtc gccagcgcgt cagtattcca ctcacgttat tgccatcccg atcgtattca
    30901 aacgtattta gcgcacaaat cttatcatta aagcctgcag tccacagcac gaatgaagat
    30961 aagttgtgcg gaaaagtgct taagaaaaca gcgttcgcac cgttttttat agctcttata
    31021 aaattcaaaa aaaaacgact ggttcacctg gccggagaga gtgccagatt tgccagtcga
    31081 attttatacg acagtataaa tgtcgttatg ccagtacagc tgaaggcgct ttgaaggcca
    31141 acggcagttc tgcgtcgtcc tggaaggtca catattccca ggcttcctgt ttcgccaaga
    31201 cagcctgcag cagtttgtta ttcagtgcat gaccggattt ataagcggta aatgcaccaa
    31261 taatattgtg accacacatg aacaagtcac cgatcgcatc gagcattttg tgacgcacaa
    31321 attcgtcttc aaaacgcagg ccgtcttcgt tcagtacgcg ataatcgtca acaacgatgg
    31381 cacaatcgaa gctgccgccc aggcacaaac cacgggactg cagatattcg atatcacgca
    31441 tgaaaccgaa cgtacgcgca cggctgatct ggcgcataaa cgcatcagcg gagaagttca
    31501 tcgcatagcg ctggttgctg gaatcaatag ccggatggtt aaaatcgatg gtgaaatcca
    31561 gcgaaaaacc attgtacggc ttaaattcag cccacttatc gccatcttcg acacgaacag
    31621 tctctttgat gcgaacaaat tttttggcgc agttcaactc gtcgataccg gcgtcaagca
    31681 gcaggtatac aaacggagcg gcgctgccgt ccatgatcgg gatttccggc gcgttaactt
    31741 cgataacaat gttatcgatg cccaagcccg cgagagcagc attgaggtgc tctacggttg
    31801 aaatccgtac atcatgctcg ttgaccagac acgtacagag catggtatca cgcacagatt
    31861 tggcatcggc cgggaaatct accggtggat tcaagtcggt gcgacgatag atgaccccgg
    31921 tgttggccgg cgcagggcgt aacgtcaggg tgactttctt gccggtatgt aaaccgacac
    31981 ccgtcgcctg aacgatacgt ttaagtgtcc tttgtttgat catcgtatta tctcgccaaa
    32041 ttacctatcc aaccgaagtg tactatacat tcggcgggcc agtttagcac aaagagcctc
    32101 gaaacccaaa ttccagtcaa ttcttaatca gcttgcttac gcaggaatgc tgggatatcc
    32161 agataatccg gctctttcgc agtttgcggc gcattgtcat tcacgacttt agcaaccggc
    32221 ttctgctcct gggtcagcgg agccatccca tgctgctggt agcgatccat cactggctgc
    32281 tgaacctgct tattggtcac cagagtgatt tcaggacgtt tgtccatgcc gatacctgtc
    32341 gcaacaacgg ttacgcgcag ctcgtcattc atatccgggt caagagaagt accgataacc
    32401 acagtcgcgt tgtcggaagc aaatgcacgg atggtgttac ctaccgtttc gaactcatcc
    32461 agacgcaggt cgaagcccgc cgtgatgtta accagcacgc cgcgcgcgcc agacaggtcg
    32521 atatcttcca gcagcggaga agagatagcc atttcagcag cttcttccgc acggtcttca
    32581 ccgctcgcca cgccagaacc catcattgcg tagcccatct cagacattac ggtgcgtacg
    32641 tctgcaaagt ccacgttcat caaacccgga cgagtaatca gttcagcgat accttgcaca
    32701 gcgcctttca gtacatcgtt cgctgcgcca aacgcatcca gcagggagat accgcggccc
    32761 agaactttca gcagtttgtc gttcgggata gtgatcagag agtccacatg cttggacagt
    32821 tcagtgatcc cctgctccgc gaatgccata cgcttcttgc cttcaaagtt gaaaggctta
    32881 gtgacgacag caacggtcag gatacccaaa tcttttgcca cttcagcgac gactggtgct
    32941 gcacctgtac cggtaccacc acccataccc gcagcaataa agaccatgtc tgcaccttcc
    33001 agcgccgcac gcaatgcatc gcgatcctca tcagccgcat tgcggccaac ttctggatta
    33061 gcgccagcgc ccagtccttt ggtgataccg ctaccgattt gaatcgtctg tccaaccgct
    33121 gttttacgca gcgcttgtgc atcggtattt accgcgaaga attcaacacc ttcaatgcgc
    33181 tcgcgcacca tgtgttcaac agcattaccg ccgccgccgc cgacgccgat gactttaatc
    33241 accgcgtcat tggtaagttc cattggttca aacatagttt ctctccgatt tgtgcctgtc
    33301 gcctgaggcc gtaatcatcg tcggcctcat aaaaattaaa actcttttcg cagccaacta
    33361 ttgagtcgct tgatccacga gccaactgat gctgtaacac gtttttctac ttcagcttca
    33421 ccgttaagat gtgactcttt cccatagtga agcaatccca ccgccgtcga ataatacggc
    33481 tcctgagcat aatccgttaa accggtaatg ttcagcggcg cgccgatacg cacttgcgta
    33541 tgaaacacgc gctgagcaca ggctgcaaga ccttcgatct gcgctgcgcc accggttaat
    33601 acaatgcctg ccgccaggtg atgtttaacc ccttgttggc gaagcttttc ctgcaactgc
    33661 aatatctctt cgttgaccag gttgagcagc tcggtatagc gcggctcgat cacctctgcc
    33721 agggtctgac gttgcagact ccgcggcgga cgaccgccta cgctcggcac ttccacgctc
    33781 tcatcttttc caacgatgga acccagcgca caaccgtggc gaactttaat cgcttcggcg
    33841 tcgcttggcg gcgtgccaaa ggcgtaagcg atatcactgg tcacgacatt gccagcataa
    33901 ggaattacct tagtgtggcg caatgcccca ccggtataaa cggcgatatc cattgtacca
    33961 ccaccgatat cgacgacgca gacacccagt tcacgttcat cttccgtcaa taccgaataa
    34021 cttgatgcca gtccggcaaa tatcagttgg tcaactttca gtccacaacg ttcaaccgct
    34081 ttgacgatgt ttttcgccat atcgttgtga catgtgatca ggtgcacttt tgcctgcatc
    34141 ctcacgcccg aaagtcctac cggattcttg atcccttcct gatagtcaat cgcatactct
    34201 tgcgggatca catgcagcac acgatgctca tcgcgcacac gcaccgattt cgcggtatgg
    34261 acgacgtttt ccacatcttc ttgcgtcact tcttcttcag aaataggcac cataccaatt
    34321 tcattctggc agctgatgtg cttaccagaa agcgccagat ataccgaaga gatctgacaa
    34381 tctgccatca attctgcctg gtcaatggca cgttgtacgc acttgaccac ggattcgagg
    34441 tcgttcaccc cgcctttatc cataccacgc gacgggcagc tgcccacgcc aatgatattg
    34501 accataccgt cgggcagaac ttcccctact aaagcggcaa ccttcgcggt accaatctcc
    34561 agtcctacta ccagttttct gtccgtcgcc ttgatcattg ttgttctgcc tgtgcctgat
    34621 tttgttgctg agtagattcc tctggcggca agggcgccca gcctactgcc gctccagagt
    34681 cataacgcaa atcaacgtag ctaatccgtt tgccatcggt ttgcgcctgc tgctgtaaaa
    34741 ccggataaag ttctacaaag cgagccaaac gtttcatcgt atcgccccgg ccaagattga
    34801 gcttaatatc gttattcagc gtcaactgcc aggaacgccg cgcggtcatc gccgcttcct
    34861 tcagagtaaa tctgtccttt gccagcatct gccccatttc gcgatagccc tgcaacactt
    34921 cattggcgct gccttccggg ccatacagca ttggaagcac ctgcttgctg gtgcgatctg
    34981 gcggcacgct gaaggtgttt ccttccgcgt ctaccatatg ttgatcattc caccgcgcaa
    35041 tcggcacata ttcaaccaga tgaatcttca attcatcagg ccactgcttt ctgacgctca
    35101 cctgcttaat ccacggcagg cgttgttcta tttgcgtctg aatgatgttg acatcctggg
    35161 tcataaaggt acccggctca cccaatgcca ggatcgactg ccggatatcg tcattacgcg
    35221 tgtaatggcg ttcaccggtc aacaccagct ttgagagcgg caggcgttgc gcatcttcca
    35281 tccagcccaa cacgacccag ccgctcacca acactgtcgt taaaacggtc agcaggaaaa
    35341 ggatccccgc cagacgcgtt ccattattgc ggcgagaaga aacctcttct tcactgtttc
    35401 gcgtgttcag agcagcctgc gacatattag tccgccagtt ccagaattcg tactaccaac
    35461 tgcgagaagc tcatacctgc ctgacgtgcc gccatcggca ccaggctgtg gctggtcata
    35521 cccggtgagg tattggcttc cagcagataa aactgtccat cgctgtccag cataacgtca
    35581 atacgtcccc atcctttgca acctaacgtc gtccatgctt tcagcactaa tgcctgcaaa
    35641 ttggcctctt gtgacgcttc cagacctgcg gggcagaaat actgtgtctc atcagagaga
    35701 tacttcgcct cataatcata gaaggttccg gacggttgaa tacgtattga cggtaaaatt
    35761 tcttcaccga gtatcgcaac cgtgaactcc ggcccactta gccatttttc aatcaatact
    35821 tcttcatcgt gctgaaatgc caatcttaat gcatcttgta gagcattttc tgctactact
    35881 tttgacattc ccacactgga accttcgcgg ctcggcttaa cgataaccgg caaacccaga
    35941 gcagaaattt ctgctaactg cttatcactc aggccttttt caaactctgc gcgggttaac
    36001 gctacccacg gcgcgaccgg taaaccggca ccttgccaca gaagtttgct gcgtagttta
    36061 tccattgaaa gcgcagatgc catcactccg ctgccggtat aaggcaagcc catcagctcg
    36121 agcatgccct gcagcgtgcc atcttcaccg ccgcgaccgt gtagcgcgat aaacactttc
    36181 tgaaagccca tcgacttcag ttgcgtcacg tcgacttctt tcgggtcgac aggatacgca
    36241 tcaataccgc cttcacgcag cccggctaac accgctgcgc cagaattcag agaaacttcc
    36301 cgctcagcgg aagtcccgcc caacaggacc gcgattttat cagtcatgtt gttcttcctc
    36361 cggagtttgc ggcttcagtt tgatttcagc taaagaacgg gcaatttttc cgatattacc
    36421 agccccctga acgagaatca ggtcgttacc ggttaatacc ggtgccagca tctcggctac
    36481 ctgcgcaggg tccggcacca gaatgggatc aattttccca cgtccacgaa ttgtgcgaca
    36541 cagcgaacgg ctgtccgctc ccggaattgg cgcttcgcca gccggataca cttccagcat
    36601 caacagggta tcaacctgcg tcagcacatt ggcgaaatca tcatacaggt cgcgcgtacg
    36661 ggtaaaacgg tgcggctgaa acagcattac caggttttta tccggccagc ctgcgcgcgc
    36721 cgctttaatg gtggcgtcaa cttccgtcgg gtggtggccg tagtcatcga ccagcattgc
    36781 cgtaccgctt ttaccattca ctggctccag cgggaattca ccgaggaaat caaaacggcg
    36841 cccagtcccc tggaagctct ccagcgcacg taaaatagcc tcgtcatcaa ttccctcttc
    36901 cgtagcaacc gcaaccgcag ctgcggcgtt cagcgcgtta tgacgacctg gcgcattcag
    36961 ggtgacgcgc atcggctctt tgtcctggcg cagcagcgta aagtgcccct gcgggccaat
    37021 ctgctgataa tcttctacac gcacgtcggc atcttcgctg aagccgtaag tcgtggtctg
    37081 acgtcccact cgcggtaaca attcgcggat caccggatca tcaacacaca tcaccgcacg
    37141 accgtaaaac ggcaggttgt gcagaaaatt aataaaagtc tgttttaaat tctcaaagtc
    37201 gccctggtag gtatccatgt ggtcggcttc gatattggtg acaatcgcca ccatcggttg
    37261 cagatgcagg aacgatgcat cactctcatc tgcttcggca atcaggtacc gaccatgccc
    37321 caaacgcgca tgaacccccg ccgcttttac cagcccgccg ttaacgaagg ttgggtcgag
    37381 ccccgcttct gcgtagatgc tggaaaccat cgcggtggtt gtcgttttgc cgtgcgttcc
    37441 tgcaatggcg atgccatgac gaaaacgcat taactcagcc agcatttcgg cacgacggat
    37501 caccggaata cgcgcttcat gagctgcgac aatttccggg ttatcggcag aaatggcgct
    37561 ggaaacaacg accacgctgg catcacgtac gttttccggg cgatggttga aataaatcgt
    37621 cgctcccaga ttcattaact gctgcgtgac cggatttggc gctaaatcgg aaccactgat
    37681 ctgataacct tcattggcca gaacttcggc aataccgccc ataccggcac caccgatgcc
    37741 gacaaaatgt atgtgccgaa cgcgacgcat ttcgggcacg atggaacgca gttttgccaa
    37801 ttgttgtgta ttcattcttt acgccattaa cttcttaaat tcatacgatg caaaaggcat
    37861 cgctacaatt acgcccgggc agcccggctc acttcatttg ccactcgctc ggtggcatcc
    37921 ggaatggatg cagcgcgggc gcgttctgcc atggttaata aggtttctcg cgaccacccg
    37981 gccagggtgt tggcgacagc atccacgcta agctgtggct gctcgataat tttggctgcg
    38041 cccgcttttt ccagcggtag cgcattccag tattgctggc ggtctttatg ttgaaacggc
    38101 acaaacaacg ccggtagtcc tgccgcggcg atttcactca ccgttaaagc accggagcgg
    38161 caaacgacga catccgccca cgcatacgcc gccgccatat catcaataaa ttccgtcact
    38221 ttatgctgcg gttgccccgc ttcggcatac gcctgttcaa cggattgttg cgaaccttta
    38281 ccgctctgat gccagatagt gactgaatca cccagtttcg cagcaacctg cggcattgtc
    38341 tggttaagaa tgcgtgcgcc ctgggaacca cccactacca gcacacgaac cggaccttca
    38401 cgtccagcca aacgttgctg cggcaacggc agcgccagca catcggtacg caccgggtta
    38461 cccaccactt ccgcattagg gaaagcgcct ggaaacgcct gcatcacttt ggtggcaatc
    38521 ttcgccagcc atttattggt taagcccgca ataccgtttt gttcatgaag tacaaccgga
    38581 atgcctaacg accacgcggc cagaccacct ggacctgata catagccgcc cataccgagt
    38641 accacgtcag gtttgtacgc tttcataatc gcccgcgcct gacgccaggc gttgaagata
    38701 cgcagcggcg cagctatcag tgcttttata ccttttccac gcagaccaga gatacgaatg
    38761 aaatcaattt cgatgccatg ttttggcact aagtccgctt ccatacggtc ggcagtcccc
    38821 agccagcgaa cttgccaacc ctgagccatt agatggtgcg caaccgccag tcccgggaat
    38881 acatgtccac cggttccgcc tgccatcacc attaatcgct ttccttgacc actcatcgtg
    38941 aacctcgtac aaacgcctgc gctttctcca gacgcgtttc ataatcaata cgcaacagca
    39001 tcatgatggc tgtcgacata atcagtaagc tcgaaccacc gtaactgatc agcggcaatg
    39061 tcagaccttt ggtcggtaac atccccgccg ccgcgccaac gttaaccagc gcctggaagc
    39121 taaaccagat gccaatagaa caggcgagaa aaccggaaaa acggtggtca atttctaacg
    39181 ctttgcggcc aatcgacatc gcgcgaaaag cgacgaagaa taccattaaa agtgccagca
    39241 ccacaccgac ataccccagt tcttcgccga taatggcgaa aataaagtca gtgtgcgctt
    39301 ccggcagata ctccagtttt tgtaccgagt tacctaaacc ttgtccccaa agttcgccgc
    39361 gaccaaacgc catcagcgat tgcgttaact gatagccgct gccaaaggga tcttcccacg
    39421 ggttccagaa tgcggtaaca cggcggatac ggtacggttc ggcgagtatc agcaacacaa
    39481 ccgctgaaat gcccataccg ataatggcaa tgaactgcca caatttcgct cccgccagga
    39541 acaacatcgc cagcgtagtc acaaacaaca ccaccaccgt accaaggtct ggctgtgcca
    39601 gcagtaacac tgccaacacc agaatcacgc ccatcggttt caggaagccg cgcaggttat
    39661 tacgtacttc gtcgccttta cgcaccagat agttggcgat ataacaaaac agcgacagtt
    39721 ttgtcagctc cgcaggctgg atacgcagca aaccgagatc gatccaacgc gatgcccctt
    39781 taaccgagct acccactacc agcacgatca tcagcaggat gatagatccg agcagcatcg
    39841 tggcactgta gcgttgccag aactccatcg gcagacgcag cgtaatgatc gccagaatga
    39901 atgccaggat cagatagacg ccatcacgct tcgcgaagaa gaacggatcg ttggttaagc
    39961 gttgccctat gggcattgac gccgaggtca ccatgataaa gccaatcgcc gcaaggccga
    40021 aggtcagcca cagtaaggtg cgatcgtaca tgatcaggct gtcggtatct ttttcccgcg
    40081 agcccatcac ccagcctttt agcgccgtgg agatccagac caggatactg aatcctggca
    40141 ggcgcggcat tttcaggcga gggagagata aacgcatcaa cctaactcct tcgccagacg
    40201 ggcaaactca ttgcctcgtt gttcaaagtt cttgaactga tcaaggctgg cacaggccgg
    40261 ggagagcaga accatatcgc ccggctgaac acgcggagcc agcaagcgca tcgcctgttc
    40321 catagtttcg gtttgttctg ccacttccgg gcgtagcgcc gccagctgcg caccgtcacg
    40381 accaaaacag tacaaacgca cgttgtcgcc attcagatag cgcgccagtg gactgaagtc
    40441 cgccgattta ccatcgccgc ccagcaacaa atgcagcgtg ccgtctacct gcaagccatt
    40501 caacgccgct tccgtactgc cgacgttagt ggctttcgaa tcgttaatcc agcgtacgcc
    40561 attgtgctcc agcaccactt caaagcgatg cggcagaccg gtgaaggtgg ttaacgcttt
    40621 caggctgctg gcgcgcggca aaccagctgc gtctgccagt gccagtgctg ccagcgcatt
    40681 ggtgtagtta tgctgcccgg aaagtttcat ctctttcacg ttcagcactt tctcgccttt
    40741 cactcgcagc caggtttctc cctgctgatg attcaggtga tagtcaccca tgttgacgcc
    40801 aaagctgacg cagcgttcat cagcaccgcg aatcggcatt gttaaggcat catcagcatt
    40861 gacgacacaa actttcgcat tttcgtaaat acgcaatttt gccgcacggt attgctgcaa
    40921 accaaacgga tagcggtcca tatgatcttc agtcacgttc agaatggtcg ccgccaccgc
    40981 ctgtaagctg gaagtggttt ccagctggaa gctcgacagt tccagcacgt acagttcgca
    41041 ctcagcatca agcagcatca acgctggcag gccaatattg ccacccacac caacgttaac
    41101 ccccgccgct ttcgccattt cacccactag cgtggtgacc gtgcttttgc cgttagaacc
    41161 ggtaatcgcc acaatcggtg cttgtgcttc gcgacagaac agctcgatat cgccaacgat
    41221 ttcgattccg gcatcagcgg cagcgcttaa ggatggatgc gccagtgcaa taccgggact
    41281 ggcgacaatc agatctgccg ccatcagcca ttcatcattc agactgcccg tgtggcgttc
    41341 tacggcttcg ggtaatttat ccaggccagg cggtgtcata cgcgtatcca taacgcgcgg
    41401 cgtcacaccg cgagcgagga aaaagtccac gcaggaaagc ccggtgaggc ccaggccgat
    41461 aatgacgaca tttttaccct gataatcagc catgcttaac gtaccttcag cgttgccaga
    41521 ccaatcagaa ccagcatcag cgaaataatc cagaaacgca caatgacgcg cggttccggc
    41581 cagcctttca gttcatagtg gtgatgaatc ggtgccatgc ggaaaatacg ttgtccgcgc
    41641 agtttaaagg agccgacctg caggatgaca gaaagcgttt ctaccacgaa cacgcccccc
    41701 ataatcacca gcaggaattc ctgacgtagc agtacggcga taatgcctaa cgcaccacct
    41761 aacgccagcg aacctacatc gcccataaag acctgcgccg gataggtgtt aaaccacagg
    41821 aagcccagtc ctgccccgac tatcgccgtg cagacaatga ccagttcccc tgcgtgtcgc
    41881 agatacggta tatgcaagta gctggcaaag ttcatattgc cggtcgccca cgccaccagc
    41941 gcaaaaccac cggcgacaaa tacggtcggc ataattgcca ggccgtcgag accatcggtc
    42001 aggtttaccg cgttgccagt acccacaatg acgaagtaag ccagcagaat gtagaacagc
    42061 cccagctgcg gcatcacatc tttaaagaat gggaccacca gctgcgttgc gggcgtgtct
    42121 ttgccggcaa ggtacagggc gaaggcgaca cccagcgcaa tgaccgacat ccagaaatac
    42181 ttccaacgag cgatcaaccc tttggtgtct ttacgcacca ctttgcgata atcatcaaca
    42241 aagccaataa caccgtaacc taccagcacc accaacacgc accagacgta cggattggac
    42301 gggtaagccc acagcagtac ggagatcaca atcgccgtca ggatcataat cccgcccatg
    42361 gtcggcgtac cgcgcttgct gaagtgtgat tcaggaccgt cgttacgcac cacctgacca
    42421 aaggaaagtt tttgcaaatg agcaatcata cgcgggccca tccacaatga gatgaacagc
    42481 gcggtcagca ggctgacgat ggcgcgaaac gtcagatagg aaaagacgtt aaagccggaa
    42541 taatatttga ccaaatgttc ggccagccaa actaacatgt cccattctcc tgtaatgcgc
    42601 gtactacctc ttccatggcg gcactacgtg aacccttaac taaaatcgta attacctgtt
    42661 gctcagcaat cagtgattta agacgcgtaa ttaacgcagt tttatcagca aaatgttcgc
    42721 caacgccgct ggcggtgctg atagcatggc tttgtttacc cacgcttaac acgcggtcaa
    42781 taccagcagc ttttgccgct tcgcccacct gtacatggca ggcttcgctt tcagcgccca
    42841 gttccgccat atcgcccacc accagcacgc ggtagcccgg catttcagcc agtacctgaa
    42901 cagcggcggt cattgaaccg acattggcgt tgtaggagtc gtcgagcagc aactggtttt
    42961 ctgccagttg gatggggaac agacggcctg gaacagcttt cagatttgcc agccccgctt
    43021 tgatagcatc aagcgttgcg cccacggaca tggagagcgc agcggctgcc agcgcattcg
    43081 caatattgtg acgccccggc aacggcagca gaacatcgac gctaccggtt ggggtttgta
    43141 gggtaaattc cgtaccgtgc gaggtcacat ggatattggt ggcggtgaaa tcgctgttgg
    43201 cggcattggg tgagaaacgc cacactttgc gtgagccaat tacgctctgc cagttcagcc
    43261 agtcgttgtt gtcggcgttc ataatggcga taccgttttc cggcaggccg ctaaagattt
    43321 cacctttcgc tttcgcgaca cccgcaagcg agccaaaacc ttccagatgc gccgctgcca
    43381 ggttgttgac cagcgcagct tccgggcgag tcagactcac agtccaggct atttcgccct
    43441 gatggttcgc gccaagttca ataactgcgt aatcgtattc cggcgttaag cgcaacagcg
    43501 tcatcggtac accgatgtcg ttgttgagat tgcctgccgt ataaagcgtg ttgccgcact
    43561 ggcttaaaat cgccgccgtc atctctttaa cggaggtttt gccggaggac cccgtcagag
    43621 caaccacgcg cgccggaact tgctggcgaa cccatgcagc cagttcacca aacgccagac
    43681 gcgtatcctt gacgattaac tgcggcaggt cgatgtccag cggacggcta accagtagtg
    43741 cgcctgcgcc gccagctttc gcctggtcgg caaaatcgtg ggcatcaaaa cgttcgcctt
    43801 tcagggcaac aaacaggcag cccggcgtca gttttcgggt atcagtggtt acagcatcaa
    43861 gggtgatatc tgcaccttgc agttcaccgt tgagaatgtc ggtaagttgg ctaagggtta
    43921 cgctaatcat gcaatcaccc ccagcagacg cgccaccgtg acgcgatcgg agtagtccag
    43981 acgctgattg ccaacaatct ggtaatcttc atggcctttg cccgcgacca gtaccacatc
    44041 attctcttta gcctgcataa cggcgcaagt caccgcttca gcacggcctt ccatcacttt
    44101 ggcatgtccg gcatctaaca ttcccgccag aatatcgttg atgatggcac gcggttcttc
    44161 ggtacgcggg ttatcgtccg tcaccaccgc cacgtcagca aactcttcgg caattgcgcc
    44221 catcagtgga cgcttacctt tatcgcgatc gccaccacag ccaaagacgc accacagctt
    44281 gcccgcacag tgcaggcgcg ccgcctgtaa ggctttttcc agtgcatccg gcgtatgcgc
    44341 gtaatccacc accaccgtcg gtttgcctgg cgcagtgaac acttccatac gtccgcaaac
    44401 cggttgcaga cgcgcggcgg ttttcagcag atcagccagt ggatagccga gtgccagcag
    44461 tgtcgccagc gcgagcagca ggttgctgac gttaaaagcg cccatcagat ggctttcaat
    44521 ttcgccatcg ccccaacttg agctaaagcg aatcgtcgca ccgctgtcgt gatagttcac
    44581 gtcgatcgct tttaaccagc gtccgtgaca gttcggattg atatgatctt ccattgatac
    44641 cgcaaccgcg tccggcagtt ttgccagcca gcggcggccc acttcatcgt cggcgttaat
    44701 aatcgcctga ccgcaatgat gttcagaata aagcagccat ttcgcagctt cgtagtgttc
    44761 catatcacca tgataatcaa ggtgatcgcg gcttaagttg gtaaagaccg acgccgcaaa
    44821 tttcaatgcc gccacacggt gctgtaccag cccgtgggag gaaacttcca ttgcgcaaaa
    44881 cgtcgcgccc tgctccacca gccccgccag ctcatgctga acatcgactg cggaaccggt
    44941 agtattttct gtcgggatca ctttcccaag cagaccgtta ccaacggtgc ccattaccgc
    45001 gctggtttcg ccaagcagtt ggctccactg cgccagcagc tgggtagtcg tggttttgcc
    45061 gttggtgccc gttacgccta cgagacgtaa attgtcagag ggttcatggt aaaagcggcc
    45121 cgccagtgca gataaacgct cgttgagctg gctgagatag atgaccggta cgccgtgcat
    45181 ttcacggatt tcaccgtcgg tcgcctcatc tttcgcctct gcaataatgg cagccacacc
    45241 ttgcgctatc gcctgcggga tatatcgacg cccgtccgcc tgatgaccta ctacagctac
    45301 aaagagatcg cccgccgcag ccacacggct gtcgagtgtc atctctcgca gtgctcgcga
    45361 aggtgcgtct ggcacccacg gagcaagaag gtcgcgcaaa ttacgatctg ccacctgtcc
    45421 cctcgccttg attaatcaca aattcatttt tatcgcccgt tgtcagcgca tccggctcga
    45481 tgttcatggt acgcaatacg ccgcccatga tggcaccaaa gaccggcgcg gaaacggcgc
    45541 cgccgtagta tttacccgcc tgcggatcgt tgataacaac aaccagcgcg aagcgcggct
    45601 gactcgcagg cgcaacgcct gcggtataag caatatattt attgatgtag cgaccgtccg
    45661 gcccgacctt tttcgcggta ccggttttaa tggcgatacg atagccttta atcgccgctt
    45721 tcacgccgcc gccgcctggt agcgccacgc tttccatcat atgcaccacg gtgcgaacaa
    45781 tggattccgg gaagacacgt tcaccgggaa ccgggggatc aactttggta atcgacagtg
    45841 ggcgataaat gccgtagctg ccgatagttg cgtagactcg cgctaactgt aacggtgtta
    45901 ccattagccc gtagccgaaa gagaaggtgg ccctctctat gtcagaccac cgttgttttt
    45961 gaggatataa gccactgcgt tctccgacca accccaaatt ggtcgctttt cccagtccaa
    46021 aacgtgagta agtatctact aacgctgagg acggcatcgc taacgccagc ttggaaacac
    46081 cgacgttact cgacttctgt aataccccgg tcagggttaa ttcgctgtag cgtgccacgt
    46141 ctttgatttc gtggccgtta attcgataag gaatggtatt gagtaccgag ttttcccgca
    46201 ccacgccacg ttgcaacgcg gtcattacca ccatcggttt aaccgttgag cccggttcaa
    46261 acacgtcggt gatggtacgg ttacgcatcg cctctttcgg cgtgccgctc agattgttag
    46321 ggttgtatga cgggctgtta gccatcgcca gcacttcacc ggtgttgaca tccaccagca
    46381 cggcgctacc ggattcagcc ttgttaaagg ccaccgcgtt gttcagttcg cgataaacca
    46441 gcgcctgcaa gcgttcatca atactcaacg ccaggttgtg cgccgcctgg ctgtcagtag
    46501 aagaaatatc ttcaattacg cgaccatagc ggtctttacg cacaatgcgc tcacccggct
    46561 gcccggtaag ccatttatcg aaactcttct caacgccctc aatcccctga ctatcgacgt
    46621 tagtaaagcc gatgaggtga gcagtcactt cgccggatgg ataataacgg cgagattctt
    46681 cacgcagatg aatccccggc agtttcagtt ttttgatgta gtccgccatg tcagggttta
    46741 cctgacgcgc cagataaata aagcgccctt tcgggttggc gttaatgcgg gcagaaagct
    46801 gatccagcgg aatattgagc gcgttagcca gcgccttcca gcggtcaccg acgctgatac
    46861 cgccagcgtc atgcacttct ttcgggtcag cccaaatcgc ttttaccggc acgctcaccg
    46921 ctaacgggcg accagaacgg tcagtaatca tgccgcggga ggtggaaact tgctgaacgc
    46981 gaagagaacg catgtcgccc tctttcacca gcatatccgg ggagataact tgtaaccacg
    47041 ctacgcgtcc gagcagaaaa gccagcgcca ggagaataca gccgcataac aacgcaaaac
    47101 gccaactaat aaagttggca tgttcttcct gacgttttgg tttctgcgtt ttcgccgctg
    47161 ctttcatgcg tcgcgtttat ccttattttt gcactacgat attttcttgt gacggatcaa
    47221 catgctgcat ttgcagcttt tccgtggcga tcctttccac ccggctatgg tcgccgagcg
    47281 cattctcttc aaggatcagg ttgcgccatt caatgtctaa agcatctcgc tccagcacca
    47341 gttgttcgcg ctgagcggtc agtaaacggg tatggtgcgc cgtggttacc acggtcaccg
    47401 ccgtcaaaat aatgcaaatg aacaggcaga gtggcagctt cccaaatcgc aaaagatcgt
    47461 caccgataac accaggcaat gcatggcgct cgtggcttcc catcgatcct ttaactttgc
    47521 ttagagcttc tgtcactctg ctgatcatgc gttcgtcctc tctgcgatac gcagaactga
    47581 actacgggca cgaggattct cagccacctc ttcttcgccc ggcattaact tgcctaatgc
    47641 tcgcagctga cggccaccca gttttttgag ctgctcttca gtcatcggta accctgccgg
    47701 aacttgcgga ccgcggctgt tttcacgcat aaaacgtttc acaatacggt cttccagcga
    47761 gtggaagctg atgatcgaaa gccgcccacc cggggccagc acgttgagcg agctttttag
    47821 cgcctgctct atctcctcca gttcactgtt tacccaaatg cgcaccgcct ggaaggtacg
    47881 agtcgcggga tgtttaaact tatctttcac cggcgttgca gcagccacga cttccgccag
    47941 ttctttggtg cgggtcatcg gctgttcgcg gttacgctcg acaatggcgc gggcaatgcg
    48001 tttggcaaaa cgctcttcac cataggtttt caatacccag gcgatatcgg cttcttctgc
    48061 ggtttgtagc cattcagcgg ctgactgccc acgggttggg tccatacgca tgtccagcgg
    48121 accatcgcgc ataaaggaaa agccgcgctc agcatcatca agttgcggtg aagagacgcc
    48181 aagatcgagg agaatgccgt cgatcttgcc gataagatcg cgctcggcaa cgtattcgcc
    48241 cagcgcggag aaaggtccgt ggatgatgga gaagcgcgga tcatcaatag tcttcgcaac
    48301 ggcgatagcc tgcgggtcgc gatcgatcgc cagcaaacgc ccctcttcgc caagctgcga
    48361 gaggatcaga cgtgagtgac caccgcgacc aaaagtccca tcaatgtaga tgccatcagg
    48421 acggatattg aggccattaa cggcttcatc cagcagcacc gtagtatgtt tatagttttc
    48481 catcatttta tagagacaag tcctgcagtc gctccgataa gtctccggta gccaactgct
    48541 ctgcgtcgat atcttccttg acctgttgat gccaggttgt ttcatcccac agctcaaact
    48601 tgttgaactg tccaaccagc atcacttctt ttgtcagccc ggcatgttgc cgcagtactg
    48661 gcgcgattaa caatcgacct gcgccatcca tctgacattc gctggcatga cctaacagta
    48721 ggcgctgcac acggcgctca accgggttca tgctcgacag acgcgataat ttttgctcga
    48781 taatttccca ttcaggcagg gggtaaagca gcaggcacgg gtgatgaatg tcaatggtgc
    48841 aaaccatttg accggcagcg ttctcaagca gctgttcccg gtaacgggta ggcactgata
    48901 agcgcccttt gctgtcgaga ttgactaacg ttgctccccg gaacatgcca gcctcacccc
    48961 ttattcccac tttgccccac aatttcccac tgaaaggagt ttacggagcg gaggaaaagc
    49021 ttgtcaagcc agtcacaagg cataccgagg taaaaaaccc gatgtttacg gctaatatca
    49081 gcgttgttta ttttacgtcc aacgtgcgca gcaaattaac gttatgaata gctgtaagaa
    49141 aaaatccctc atcgtcatca ccgttttaaa atgactcact attttccaca gcaaatataa
    49201 agtgtcagtt tgcgacgcga gcggcatttt aggacatatc ttccccggtt aacagtccct
    49261 gttgcgtctg tgtggcgctt gatgccagcg ggtttgcgca aaagcaaaga aaagtgcttg
    49321 ttaaatcggc aattcatctc tgcatgtaac aaaataatgc aaaaacgcgc tgtcgatatc
    49381 acttaattat tgtgaatttc agctggtgag ttaattaacg ggcgggttta aaagaactta
    49441 tcaataatag tcaggaatag tcttatttac tttaaggata ttgatgtcca ttcccgtact
    49501 ctacgcgcca gagggaaatt cacctggcgc gtatttttgt tcgcggctta gctacggctg
    49561 agcacaccgc gacgatagag attacgttta atgcgcgtta aaccaggttt tggcttacgc
    49621 ggttcgtcca ggcttgccag gacaatctcc agcacacgct ctgcgacatc gcggtgacgt
    49681 tgagccactg ccagcaccgg gcactgtaag aagtcgagca gttcgttatc gccaaaggtg
    49741 gcaattgcca ggtcagaagg cagtttacca tcgcgacgta gcgttacatc catcacccct
    49801 tgcaacaacg caaacgacgt tgtgaacagc gcctgcggca tcggatgcgt ttccagccat
    49861 ttttcgaata actgggcagc cgcctcccgc tcatagctgt tggcatacag gaaatgcact
    49921 tcgcgcggat catctttcca ggcagtacgg aaaccttgtt cacgcaggaa gctgacagaa
    49981 agctccggca gcgcgccaag ataaagcacc gtctcggcgg gaaacttacg taactcttcc
    50041 gccagcattt cggcatcatc ctgatcggca ccaaccacgc tggtgaagtg ttcacgatcg
    50101 agggcgcggt ccagcgcgac aatcgggaac gggtcgttag cccagcgttg ataaaaagga
    50161 tgctcaggag gcaacgacgt cgaaacaata atggcatcga cctgacgctg taaaaggtgc
    50221 tcaatacagc gcatttcgtt gtctggctga tcttctgagc aggcaattag cagttgataa
    50281 ccccgttgcc gcgcctggcg ttcaagatag ttagcgatgc gggtatagct ggtgttctcc
    50341 agatcgggga tcacaagacc aatagaacgt gtgcgtccag cacgaagccc agctgccacg
    50401 gcgttcgggt ggtaattgtg ctcacgcacc acagccatga ctttttcaac ggttttgtcg
    50461 ctcacacggt attgcttcgc tttgccgtta ataacatagc ttgcagtggt ccgcgacact
    50521 cccgccagcc gagcgatttc atccagtttc acaattgccc cttgcgtaaa atgtaaaaac
    50581 catagccatt gtactggtat gggttaaatt cattaacatc taagcgcaga aaattaactg
    50641 cggcaactgc ttttattcca ggttcccact gatttcgcaa aaaaagccca acgtgacatg
    50701 ttgggctgca aattgcgcac tgagatcatt ctcaacgcat tattttatcg ccgcgcgaaa
    50761 gtccgaccac accagagcga gcaacttcca caattttcgc cacatcgcga atcgatgcta
    50821 aaaatgcatc aagcttatcg ctggtgcctg ctaattgaac ggtataaagc gagggggtga
    50881 catcgataat ttgcccacgg aatatttccg tattacgttt cacttcgtca cgcccgtaac
    50941 cgctggcctg aattttcacc agcatgattt cccgctcaac atgcgcgccc tgccccaact
    51001 cactcacgcg caagacatcg accagcttgt gtaattgctt ttcgatctgc tcaagcactt
    51061 tttcatcgcc cacggtctgg atggtcatac gcgataatgt cggatcgtcg gttggcgcaa
    51121 cggtcaggct ttcaatgttg tagccacgct gggaaaaaag gccaatcacg cgggataacg
    51181 cgcctgattc attttcgagt aagactgata atatccggcg cataatcagg ttctctccgt
    51241 tttgcttaac cacatttcat ccattccgcc cccgcgaatc tgcatcgggt agacgtgctc
    51301 actgccatcg acggtaacat caacaaacac caggcgatta ttgcgcacct gttccagcgc
    51361 ctcgctaagt ttgctttcca gctcatgcgg atgagagatc tggatcccga cgtgaccata
    51421 ggcttccgcc agacggacga aatcgggtag tgattgcata taagattgtg aatgacggcc
    51481 ggaatagatc atgtcctgcc actgtttcac catccccaga tagcggttat tgagattcac
    51541 caccagtacc ggcaactcgt attgcaacgc ggtagacagt tcctggatgt tcatctgaat
    51601 actgccgtcg ccagtgacgc aaaccacggt ttcttctggc aacgccattt tgacgcccag
    51661 tgccgcaggt aaaccaaaac ccatcgtgcc gaggccaccg gaattgatcc agcgacgcgg
    51721 tttgtcgaat ggataataaa gtgcagcaaa catctggtgc tgcccgacat cggacgtcac
    51781 gtaagcgtct cccttcgtca accgccaaag agtctcgatc accgcctgcg gtttaatctt
    51841 ttcactgtga gtgtcatatt tcaggcactg acgagcgcgc cactgttcaa tctgctgcca
    51901 ccagtcgcgg atctcatcca gcggttgatg ggcggattct tgcgacagga gttcaagcat
    51961 ttgttcgagg acctggcgag catcccccac aatcgggata tcagcagtca cggttttaga
    52021 aatggaagta ggatcaatat cgatatgcag aacagtggca tttgggcagt actttgccag
    52081 attattcgtc gttcggtcat caaatcttac cccgacggcg aaaatcacat ccgcgttatg
    52141 catcgtcata ttggcttcgt aggtgccgtg cattcccagc atgcccagcg cctgacgatg
    52201 cgttgccgga aatgccccca ggcccatcaa tgaggaaaca acgggcagat tcaacgcctc
    52261 caccgtttct ttcaactgct gatggcagcc cgccgtgatt gccccaccgc ctacgtagac
    52321 aaccggtttt tttgccgcca ccagcgtttg cagagcacgc ttaatttgcc ctttatgtcc
    52381 ggtagtagtg ggattgtaag aacgcatact aaccgactcc ggccagacat agggtaattt
    52441 gttcgccgga ttaagaatat ctttcggtaa atcaacgact actggtccag gacgaccact
    52501 tgccgccagc cagaaagcct ttttcagcac ctgcggaatg tcttccgttt gcttaaccag
    52561 aaaactgtgt ttaaccaccg gtcgcgaaat ccccaccatg tcgcactcct gaaaggcatc
    52621 gtaacctatc aacgaggtcg ctacctgccc ggaaaggaca actaatggaa tggaatccat
    52681 ataagcggtg gcgatgccgg taatcgcatt ggtcgcccct ggacccgacg ttaccagcac
    52741 gacgccgact tccccggtcg cacgcgccag gccatcggcc atatgcaccg ccgcctgctc
    52801 atgacgaact aatacatgat caataccacc cacggtatgc aatgcatcgt aaatatcaag
    52861 gactgcgcct ccgggataac cgaatacttg tttaacgccc tgatcgataa gcgatcggac
    52921 gaccatctcg gctccagaca acatctccat ggcctgcctc actgtttgac ggaaaaaatg
    52981 tgtaaaacac attaaccgtc cgatcttgct taagcaattg gcagccagag acataaacgc
    53041 agggtaataa gaataaacag gaggaaaggt gaaaagaaac gataaaatcc tccattacga
    53101 aggattaaat tgacaataaa tcggctgaat cccacaactt acagcaattt ctctattttt
    53161 ttaattaatc ctaaaataga gagcacacac attcagaata aaattctaca ttcaataaaa
    53221 aatgcagaat aaaccagaca ttcatgtctg gcctattctg cattcagtta gcgtttgcaa
    53281 attgagacta attgttcttc catccactga tggcctttat cgcgcccggc agcttcatgc
    53341 caggagagat aacaggttct gctgttttgt tttaacggca gcggtaatac ctgtaattct
    53401 aaggattcag cgaactcttc agccagccaa cgcggcgcaa tagcgaccaa atgcgtttgc
    53461 gacaccacgc taagtacgct catcattgcc atgccctgat acgcgatact ggcttgctta
    53521 tctaccgtgt cataccaagg ttgactaaat gacgcgaaac gttcgagcga aaccgccgca
    53581 tgttgttcgt tataaacatc atgtttcagt aacgggccct taattgttgg atgatttttg
    53641 ctggctacca gcaccatttc atctttaaat aatggcacgc tggtaaattc aggacgatgg
    53701 aagtcttcat aactaatcac aaactccgtt tcctgataac gcagctgatg ttcagtgttc
    53761 tgatttaatg aagacttgaa cataacatgt atatttggcg caatctgctc aatgtgatta
    53821 taaatctgcg aggtcagaat gctgtctaac gggctgcaaa cacaaagatg aaatacacgt
    53881 tcactgctcg cgggttcaaa acctgaacca ggcaattcat tttgtactag ttgcaatgcc
    53941 tgacgaactg agccaaaaag ttgaaatgcg cgagcagtcg gttgaatacc acggccataa
    54001 cgaacaaaaa gctcgtcatt aaacatcacc ttcaggcgtg caacagcgtt actgaccgca
    54061 ggttgcgaca tacccagaac atgagcggca cgggtaatgt tttgctcctg catcacggca
    54121 tcgaaaacgg ttaataagtt gagatcgacc atgcgtagct gtggcttgct taactccgcc
    54181 gtctctgaat gatctgtttg tacctctggc atacttaact ccactgtcac acttaactcc
    54241 ctttccctta ttggaatgca gagaaataat cctgaagaat atgatttatc atgcatataa
    54301 aataagaaaa agcaaaatga gtaaaattcg gaaaacataa agatacgggt tctgataaaa
    54361 acagaatcca ttgccgtgga atgagtcatt taccacagca taataatcca taatgttttt
    54421 gttgcgaaaa caatctaatc ataactaccg cgaatactca atcatctaca aaatggatta
    54481 aatgtgctta tttaataatt aatttatata tttaatgcat taattcttaa cattaattga
    54541 tcaataatat tcaccaaatc aatatcaaaa aaaatcgcaa aacatataat tcaatacaaa
    54601 tcatcaggat aggttttgca acgcgtgcat tttgtcccct ttttcctcgt tgattagatg
    54661 caaaaattta tgctgaaata tgtcaaccga tgaaaagcgt cggtagttaa gcagaaatta
    54721 atatcgctta ctttaaccac cgcagcacaa ttagctaatt ttacggatgc agaactcacg
    54781 ctggcgggac gtttttattg cgtcagggtt gacatccgtt tttgtatcca gtaactctaa
    54841 aagcatatcg cattcatctg gagctgattt aatgactcac atcgttcgct ttatcggtct
    54901 actactacta ctaaacgcat cttctttgcg cggtagacga gtgagcggca tccagcatta
    54961 agccagcacg cagtcaaaca aaaaacccgc gccattgcgc gggttttttt atgcccgaag
    55021 cgaggcgctc taaaagagac aaggacccaa accatgagcc agcaagtcat tattttcgat
    55081 accacattgc gcgacggtga acaggcgtta caggcaagct tgagtgtgaa agaaaaactg
    55141 caaattgcgc tggcccttga gcgtatgggt gttgacgtga tggaagtcgg tttccccgtc
    55201 tcttcgccgg gcgattttga atcggtgcaa accatcgccc gccaggttaa aaacagccgc
    55261 gtatgtgcgt tagctcgctg cgtggagaaa gatatcgacg tggcggccga atccctgaaa
    55321 gtcgccgaag ccttccgtat tcataccttt attgccactt cgccaatgca catcgccacc
    55381 aagctgcgca gcacgctgga tgaagtgatc gaacgcgcta tctatatggt gaaacgcgcc
    55441 cgtaattaca ccgatgatgt tgaattttct tgcgaagatg ccgggcgtac acccattgcc
    55501 gatctggcgc gagtggtcga agcggcgatt aatgccggtg ccaccaccat caacattccg
    55561 gacaccgtgg gctacaccat gccgtttgag ttcgccggaa tcatcagcgg cctgtatgaa
    55621 cgcgtaccta acatcgacaa agccattatt tccgtacata cccacgacga tttaggcctg
    55681 gcggtcggca actcactggc ggcggtacat gccggtgcac gccaggtgga aggtgcaatg
    55741 aacgggatcg gcgagcgtgc cggtaactgt tcgctggaag aagtcatcat ggcgattaaa
    55801 gttcgtaagg atattctcaa cgtccacacc gccattaatc accaggagat atggcgcacc
    55861 agccagttag ttagccagat ttgtaatatg ccgatcccgg caaacaaagc cattgttggc
    55921 agcggcgcat tcgcacactc ctccggtatc caccaggatg gcgtgctgaa aaaccgcgaa
    55981 aactacgaaa tcatgacacc agaatctatt ggtctgaacc aaatccagct gaatctgacc
    56041 tctcgttcgg ggcgtgcggc ggtgaaacat cgcatggatg agatggggta taaagaaagt
    56101 gaatataatt tagacaattt gtacgacgct ttcctgaagc tggcggacaa aaaaggtcag
    56161 gtgtttgatt acgatctgga ggcgctggcc ttcatcggta agcagcaaga agagccggag
    56221 catttccgtc tggattactt cagcgtgcag tctggctcta acgatatcgc caccgccgcc
    56281 gtcaaactgg cctgcggcga agaagtcaaa gcagaagccg ccaacggtaa cggtccggtc
    56341 gatgccgtct atcaggcgat aaaccgcatc actgactata acgtcgaact ggtgaaatac
    56401 agcctgactg ctaaaggtca cggtaaagat gctctgggtc aggtggatat tgtcgccaac
    56461 tacaacggtc gccgcttcca cggcgtcggc ctggccaccg atattgtcga gtcctccgcc
    56521 aaagccatgg tgcacgtact gaacaatatc tggcgcgccg cagaagtcga aaaagagttg
    56581 caacgcaaag ctcaacacaa cgaaaacaac aaggaaaccg tgtgatgtcg aagaattacc
    56641 atattgccgt attgccgggg gacggtattg gtccggaagt gatgacccag gcgctgaaag
    56701 tgctggatgc cgtgcgcaac cgctttgcga tgcgcatcac caccagccat tacgatgtag
    56761 gcggcgcagc cattgataac cacgggcagc cgctgccgcc tgcgacggtt gaaggttgtg
    56821 agcaagccga tgccgtgctg tttggctcgg taggcggccc gaagtgggaa catttaccac
    56881 cagaccagca accagaacgc ggcgcgctgc tgcctctgcg taagcacttc aaattattca
    56941 gcaacctgcg cccggcaaaa ctgtatcagg ggctggaagc attctgtccg ctgcgtgcag
    57001 acattgccgc aaacggcttc gacatcctgt gtgtgcgcga actgaccggc ggcatctatt
    57061 tcggtcagcc aaaaggccgc gaaggtagcg gacaatatga aaaagccttt gataccgagg
    57121 tgtatcaccg ttttgagatc gaacgtatcg cccgcatcgc gtttgaatct gctcgcaagc
    57181 gtcgccacaa agtgacgtcg atcgataaag ccaacgtgct gcaatcctct attttatggc
    57241 gggagatcgt taacgagatc gccacggaat acccggatgt cgaactggcg catatgtaca
    57301 tcgacaacgc caccatgcag ctgattaaag atccatcaca gtttgacgtt ctgctgtgct
    57361 ccaacctgtt tggcgacatt ctgtctgacg agtgcgcaat gatcactggc tcgatgggga
    57421 tgttgccttc cgccagcctg aacgagcaag gttttggact gtatgaaccg gcgggcggct
    57481 cggcaccaga tatcgcaggc aaaaacatcg ccaacccgat tgcacaaatc ctttcgctgg
    57541 cactgctgct gcgttacagc ctggatgccg atgatgcggc ttgcgccatt gaacgcgcca
    57601 ttaaccgcgc attagaagaa agcattcgca ccggggattt agcccgtggc gctgccgccg
    57661 ttagtaccga tgaaatgggc gatatcattg cccgctatgt agcagaaggg gtgtaatcat
    57721 ggctaagacg ttatacgaaa aattgttcga cgctcacgtt gtgtacgaag ccgaaaacga
    57781 aaccccactg ttatatatcg accgccacct ggtgcatgaa gtgacctcac cgcaggcgtt
    57841 cgatggtctg cgcgcccacg gtcgcccggt acgtcagccg ggcaaaacct tcgctaccat
    57901 ggatcacaac gtctctaccc agaccaaaga cattaatgcc tgcggtgaaa tggcgcgtat
    57961 ccagatgcag gaactgatca aaaactgcaa agaatttggc gtcgaactgt atgacctgaa
    58021 tcacccgtat caggggatcg tccacgtaat ggggccggaa cagggcgtca ccttgccggg
    58081 gatgaccatt gtctgcggcg actcgcatac cgccacccac ggcgcgtttg gcgcactggc
    58141 ctttggtatc ggcacttccg aagttgaaca cgtactggca acgcaaaccc tgaaacaggg
    58201 ccgcgcaaaa accatgaaaa ttgaagtcca gggcaaagcc gcgccgggca ttaccgcaaa
    58261 agatatcgtg ctggcaatta tcggtaaaac cggtagcgca ggcggcaccg ggcatgtggt
    58321 ggagttttgc ggcgaagcaa tccgtgattt aagcatggaa ggtcgtatga ccctgtgcaa
    58381 tatggcaatc gaaatgggcg caaaagccgg tctggttgca ccggacgaaa ccacctttaa
    58441 ctatgtcaaa ggccgtctgc atgcgccgaa aggcaaagat ttcgacgacg ccgttgccta
    58501 ctggaaaacc ctgcaaaccg acgaaggcgc aactttcgat accgttgtca ctctgcaagc
    58561 agaagaaatt tcaccgcagg tcacctgggg caccaatccc ggccaggtga tttccgtgaa
    58621 cgacaatatt cccgatccgg cttcgtttgc cgatccggtt gaacgcgcgt cggcagaaaa
    58681 agcgctggcc tatatggggc tgaaaccggg tattccgctg accgaagtgg ctatcgacaa
    58741 agtgtttatc ggttcctgta ccaactcgcg cattgaagat ttacgcgcgg cagcggagat
    58801 cgccaaaggg cgaaaagtcg cgccaggcgt gcaggcactg gtggttcccg gctctggccc
    58861 ggtaaaagcc caggcggaag cggaaggtct ggataaaatc tttattgaag ccggttttga
    58921 atggcgcttg cctggctgct caatgtgtct ggcgatgaac aacgaccgtc tgaatccggg
    58981 cgaacgttgt gcctccacca gcaaccgtaa ctttgaaggc cgccaggggc gcggcgggcg
    59041 cacgcatctg gtcagcccgg caatggctgc cgctgctgct gtgaccggac atttcgccga
    59101 cattcgcaac attaaataag gagcacacca tggcagagaa atttatcaaa cacacaggcc
    59161 tggtggttcc gctggatgcc gccaatgtcg ataccgatgc aatcatcccg aaacagtttt
    59221 tgcagaaagt gacccgtacg ggttttggcg cgcatctgtt taacgactgg cgttttctcg
    59281 atgaaaaagg ccaacagcca aacccggact tcgtgctgaa cttcccgcag tatcagggcg
    59341 cttccatttt gctggcacga gaaaacttcg gctgtggctc ctcgcgtgag cacgcgccct
    59401 gggcattgac cgactacggt tttaaagtgg tgattgcgcc gagttttgct gacatcttct
    59461 acggcaatag ctttaacaac cagctgctgc cggtgaaatt aagcgatgca gaagtggacg
    59521 aactgtttgc gctggtgaaa gccaatccgg ggatccattt cgacgtggat ctggaagcgc
    59581 aagaggtgaa agcgggagag aaaacctatc gctttaccat cgatgccttc cgccgccact
    59641 gcatgatgaa cggtctggac agtattgggc ttaccttgca gcacgacgac gccattgccg
    59701 cttatgaagc aaaacaacct gcgtttatga attaatcccc ttgcccggtc aaatgaccgg
    59761 gctttccgct atcgtccacg tcatcaaacg tctttaacct ttgcggttaa aaataatgcg
    59821 acaacagaaa taaccgcaat tacccagtag acagcaaagt gcccccaact ttgtgcaatt
    59881 gctccctgaa taacgccagc cagaattacc ccggtagaaa tactgttagt aaataaggtg
    59941 gtagctgccc ccgctcttcc aggcattaaa tcctgaaacc atagcatccc aatacccgca
    60001 acaatgccga taaatacagc gttaaaaagt tgcagcgtca tcaacgccat acggctatga
    60061 aagaaaatca atccggtgta aaacagtact cctgccgcca ctgctatgac catcattcgc
    60121 cgcttaccat aacgtttgac atagtagcca gccagaatca ttgctggtat ttccagtcca
    60181 gctgccgtcc ccatcaggaa acccgccagt ttgtctggca atcctaactc gctactgatc
    60241 cacaacggca tatcaataat gtacatggtg ttgcaggtcc acattaacgt cgaggcgaca
    60301 aataacatcc gtacgttact atcctgccag ccaccttgca ttgataaagc attttccgac
    60361 ggcagttcta cccgcgccac agacggaagc ataaatgcaa tcaataccag actgagtgtg
    60421 aatatcccgg cggcaatcga aaacatcacc gtaaagccgt aattcaacgc cagcataaag
    60481 gccaacggtg gaccgataac ccatgccaga gaaagctgcg cacgcatcac cgagctaaac
    60541 atcaccactt ctcgcgccga gttatccgca tattcccgcg ccagagcaaa taactgtggc
    60601 attgccgtat tggccagaga tgccagaagc acaccacagg tgataagcgt cagataatga
    60661 cgattaaatg caaacaatag cgcattgccg atagccatca aacagcaaaa tataatcagt
    60721 tttcgccgat cgccctgact gtcagaacgt tttgccaacc agaggcttac gccgatccca
    60781 gcaatagcat tcaccgtata aaagagtccg atccagaaag gttgcgcgcc aacctcacga
    60841 ctcagaaata agctcaatgt aggagcctgt agcgccccgg ccacccccat cataaaagcg
    60901 accagcataa atgccgcgta aacaccgttc atacggcgag ccatcgtcat tatccagatc
    60961 atacgttccc tttttagcgc ggcgagaata aaaaaaacca gcaggtataa tctgctggcg
    61021 ggtgatttta caccaatact cagtcacaca tgatgcaggc aagtcaactt tcagaattgc
    61081 ggtcatccca ctgcatcagt ccttcaagca tggttaatcg ttgtgggacg cttaaccaac
    61141 gcaaccagca caacttcgct gtcgcggtaa aatagtgctg ataaaactga cgcatggggc
    61201 acctccttgc ttcatcgtta ggaattattg acttaatata gggaaaataa aattgctgtc
    61261 ttttgcgcag gagttcccct tttatgccat ctgctcgtct gcaacaacag ttcatccgcc
    61321 tgtggcaatg ctgcgagggt aaatcgcagg acacaacgct caacgaactg gcagcgttat
    61381 tgagctgctc gcgtcgtcat atgcgtaccc tgctcaacac catgcaggat cgcggctggc
    61441 tgacgtggga agcggaagtc gggcgcggta aacgctcgcg tctgacattc ctctataccg
    61501 ggctggcgct tcagcaacag cgggcggaag acctgctgga gcaggatcgt atcgaccaac
    61561 tagtgcagtt ggttggcgac aaagcgactg tgcggcaaat gctggtttct catctgggcc
    61621 gcagcttccg ccaggggcgg cacatcctgc gcgtgctcta ctatcgtccg ttgcgtaatc
    61681 tgctacctgg cagcgcattg cgccgttccg aaacccatat tgtccggcaa atcttcagtt
    61741 cgctaacgcg cataaatgag gaaaatgggg aactggaagc agacatcgcc caccactggc
    61801 agcaaatatc accgcttcac tggcgtttct ttttgcgtcc aggagtccat tttcatcatg
    61861 gtcgtgaact ggaaatggac gacgtgatcg cctctttaaa acgaatcaat acgctgccgc
    61921 tctattcgca tattgcggac attgtgtcgc cgacgccctg gacgctggat atccatctca
    61981 cgcaaccgga ccgctggtta ccgttactgc tgggacaagt tccggcgatg atcctgccgc
    62041 gcgaatggga aaccctcagt aactttgcca gccatcccat cggcaccggt ccgtatgcgg
    62101 tgatccgcaa caccaccaat caactgaaaa ttcaggcatt cgatgacttc ttcggttacc
    62161 gggcattaat cgatgaagtt aacgtctggg ttctgccgga aattgccgac gagccagctg
    62221 gaggtctgat gctaaaaggt ccacagggcg aggaaaaaga gattgaaagc cgcctggagg
    62281 aaggttgcta ctatttactg ttcgacagcc gcacccatcg cggggcgaat cagcaagtca
    62341 gggactgggt aagctatgtg ctttctccaa ctaatctggt ctatttcgct gaggaacagt
    62401 accagcaact gtggttcccg gcttatggac tgctcccccg ttggcaccat gcccgcacca
    62461 taaagagcga aaaaccggct ggcctggaaa gcctcaccct gaccttttat caggatcaca
    62521 gtgagcatcg ggtgattgcc gggatcatgc agcagattct ggcaagtcac caggtcacgc
    62581 tggaaatcaa agagatcagc tacgatcagt ggcatgaagg agagatcgag agcgatatct
    62641 ggcttaatag cgccaacttt acgctgccgc tggatttttc gctgttcgcg cacttgtgcg
    62701 aggtgccgct gctccaacac tgtattccga tcgactggca agccgacgcc gcccgctggc
    62761 gcaatggcga aatgaacctg gcgaactggt gccagcaact ggtcgccagt aaagcaatgg
    62821 tgccgcttat ccaccactgg ctgatcattc agggacaacg cagtatgcgc ggcctgcgta
    62881 tgaataccct cggctggttc gattttaaat cagcgtggtt tgcgccgccg gatccatgat
    62941 tgctggtaga taacaaaatc actacactaa cgccgttctc aacggggtgc cacgcaaacg
    63001 cgtgcgctga gaaaataccc gtcgaacctg atccggataa cgccggcgaa gggatttgag
    63061 gctccttctc aagtcctttg ccactctttt ttgaggtgca aagtgttaaa aaaatgtctt
    63121 cccctgctgt tgctgtgcac agcgcccgtt ttcgctaaac ccgttctgac tgtttatacc
    63181 tacgattcct tcgccgccga ctgggggcct ggtccaaaga tcaaaaaagc ttttgaagcc
    63241 gactgtaact gcgaactgaa actggtggcg ctggaagatg gcgtttcgct tctcaaccgt
    63301 ctacggatgg aaggcaaaaa tagtaaagcc gatgtggtgc tggggctgga taacaatctg
    63361 ttagacgccg ccagtaaaac cggactattt gccaaaagcg gtgtggcagc ggatgccgtt
    63421 aacgttcccg gcggctggaa taatgacact ttcgtcccgt ttgattacgg ctatttcgcc
    63481 ttcgtctatg acaagaacaa actgaaaaac ccgccgcaaa gcctgaaaga attggttgag
    63541 agcgatcaaa actggcgggt gatttatcag gatccgcgca ccagtacgcc ggggctgggc
    63601 ctgctgttgt ggatgcaaaa agtctatggc gatgacgccc cacaagcctg gcaaaaactg
    63661 gcgaagaaaa ccgtcacggt caccaaaggc tggagcgaag cctacggcct gtttttaaaa
    63721 ggtgaaagcg atctggtact gagttacacc acctctccgg cttatcacat tctcgaagag
    63781 aaaaaagata actacgccgc cgcgaacttt agcgaaggtc actatctgca ggtggaagtc
    63841 gccgcccgca ccgctgccag caagcagccg gagctggcgc aaaaattcct ccagtttatg
    63901 gtttctccgg cttttcagaa tgcgatccca accggtaact ggatgtatcc ggtggcaaat
    63961 gtcacgctgc ctgccgggtt tgaacaattg accaaaccgg ccaccacgct ggagttcaca
    64021 ccagctgaag tggctgcaca acgtcaggca tggattagcg aatggcaacg cgccgtcagc
    64081 cgttaattcc cggctggtta attccaggtg taagcgccgc cacgctggtg gtagcggttg
    64141 cgctcgcggc gtttctcgcc ctgtggtgga acgcgccgca gggtaactgg gtggcagtct
    64201 ggcaggacag ctacctgtgg catgtggtgc gcttctcctt ctggcaggcg tttctctcgg
    64261 cgctactctc tgtcgtaccc gcgatattcc tcgcccgcgc cctctatcgc aggcgctttc
    64321 cgggtcggct ggcgctgttg cgtctgtgcg caatgacctt gatcctcccg gtgctagttg
    64381 ctgttttcgg cattcttagc gtctatggtc gtcagggctg gctggcatcg ctctgccaat
    64441 cgctcggtct ggagtggacc ttttcgccct acggcctgca aggcattttg ctggcgcacg
    64501 tattttttaa tctgccgatg gcgagccgct tattactcca ggcactggaa aacattcccg
    64561 gcgaacaacg tcagcttgcc gcccagcttg ggatgcgcgg ctggcatttt ttccgcttcg
    64621 tcgaatggcc gtggctacgg cgacaaatcc cgccggttgc cgcgcttatc tttatgctct
    64681 gtttcgccag cttcgccacc gtgctatcgc tgggcggcgg tccgcaggca actactatcg
    64741 agctggctat ttatcaggcg ctgagttacg actacgatcc tgcccgcgcg gcgatgctgg
    64801 cgctgctcca gatggtgtgt tgccttgggc tggtgctgtt gagtcagcga ctaagtaagg
    64861 ccattgcgcc cggtaccacg ctgctgcaag gctggcgcga tccggacgat cgtctgcata
    64921 gccgcatttg cgacacggtg ttaatagtgc tggcgctgct gctgttgctg ccaccgttgc
    64981 tggcggtgat cgtcgatggg gtaaatcgcc agttgccgga agtgctggca caaccggtgc
    65041 tgtggcaggc gctgtggacc tcgttgcgta ttgcgctggc ggcaggtgta ttgtgcgtag
    65101 tgctgaccat gatgctgcta tggagcagtc gcgaactgcg ggcgcggcag aaaatgctgg
    65161 cgggtcaggc gctggagatg agcggcatgt tgatcctcgc catgccgggg attgtgctgg
    65221 ctaccggctt ctttttactg ctcaacaaca ccattggcct gccgcaatct gctgacggca
    65281 ttgtgatttt caccaatgcg ttaatggcga tcccttatgc gctgaaagta ctggaaaacc
    65341 cgatgcgcga tatcaccgcc cgctacagta tgttgtgtca gtcgctgggc attgaaggct
    65401 ggtcgcgctt aaaagtggtc gagctgcgcg ccctgaaacg tccactggcg caggcgctgg
    65461 cttttgcctg cgtgctgtcg attggtgatt ttggcgtagt ggcgttgttc ggtaacgatg
    65521 atttccgtac cctgccgttt tatctctacc agcaaattgg ctcctatcgc agccaggacg
    65581 gtgcggtcac cgcgttaatt ctgctactac tctgttttct gctgtttacc gtgattgaaa
    65641 aactaccggg gcgaaatgtt aaaactgact gatatcacct ggctttatca ccatttgccg
    65701 atgcgtttta gcttaacggt agaacgcggc gagcaggtgg caatcctcgg gccaagcggc
    65761 gcgggtaaaa gtaccctgct gaatttgatc gccggttttc tgacgccagc cagcggttcg
    65821 ctgactatcg acggcgttga tcacaccacg acgccgccgt cacgccgtcc ggtgtcgatg
    65881 ctgtttcagg aaaacaacct gttcagtcac ctgacggtcg cgcagaacat cgggctgggg
    65941 ctaaatccgg gattgaaact gaacgcggca cagcaggaga aaatgcacgc tatcgcccgc
    66001 cagatgggga ttgataattt aatggcgcgg ttacctggcg agctttccgg cggtcagcga
    66061 cagcgcgtgg cgttagcgcg ttgtctggtg cgcgaacagc cgattttatt gctcgatgaa
    66121 ccgttctctg cgctcgatcc ggcgttacgt caggagatgc tgacgctggt gagcacgagc
    66181 tgccagcagc aaaaaatgac gctattgatg gtgtcgcaca gtgtggaaga tgcggcgcgg
    66241 atcgccacac gctcggtggt ggtcgccgac gggcgcatcg cctggcaggg taagaccaat
    66301 gagttgttga gcggtaaggc gagtgcttcg gcactattgg ggattacggg ttagtatgca
    66361 ctgtcggatg cggcgtgaac gccttatccg acctacgggt agtgcgtttt gtaggcctga
    66421 taagacgcgg tcagcgtcgc atcaggcaac ccgcacaaga ctatctccta acccccaacc
    66481 actttacgca aaatatcgat atacaccggc atcaacgggt ggcgaattaa caccaccagc
    66541 gccaccacgc cgatggcaga aattaacggc gtcagccaca acaaacgacc acgggacaaa
    66601 taatgactca aacggtcagt cgctttacca ctgcgccata accgccagca cagccagcca
    66661 ccaacccaca aaaacaccgc tgttgccagc agcaaccatt taaactcacc gctctgcatt
    66721 ccggctggaa tatcgatcgc cgcgcccgcc agaatccctg gcaggaagta aaacggtggc
    66781 cacagcaggc agccgataat attcggcgta atgaattttg ccaccggcag atccagcatt
    66841 cccgccacca ttggcaccag cggacgcgtc gggccaacaa aacgaccgac cagaatggtg
    66901 aacatgctgt gttgatgcaa cgcatgttca gttttatcga gtaatgcttt gtttttcttc
    66961 agaaatgacc agcgatgcaa cggcttttta aaacgccagc ccagccagaa ggagatccag
    67021 tcgcccagca ggcaaccgac gatccctgcc agccaggcgt gccagaaact taactcgccg
    67081 ctgccaatca gcgctcccag ccccgccatc agcaccgtac cgggtagaat caaaccgacc
    67141 agcgccagcg actccagaaa ggccaccaac accaccgcca tcaatgaata cacggtagat
    67201 tgggtaataa agtgttccag caatgcttgc ataatgtgcc tgtcaaatgg acgaagcagg
    67261 gattctgcaa accctatgct actccgtcaa gccgtcaatt gtctgattcg ttaccaatta
    67321 tgacaacttg acggctacat cattcacttt ttcttcacaa ccggctcgga actcgctcgg
    67381 gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt caaaaccaac
    67441 attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct tcgcctggct
    67501 gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc ggaaaagatg
    67561 tgacagacgc gacggtgaca agcaaacatg ctgtgcgacg ctggcgatat caaaattgct
    67621 gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat ccatcggtgg
    67681 atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa gcagatttat
    67741 cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt gcccaaacag
    67801 gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaacccgtat tggcaaatat
    67861 tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa cccactggtg
    67921 ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg gcgggaacag
    67981 caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca ccccctgacc
    68041 gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga taaaaaaatc
    68101 gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat taaacgagta
    68161 tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc cgccattcag
    68221 agaagaaacc aattgtccat attgcatcag acattgccgt cactgcgtct tttactggct
    68281 cttctcgcta acccaaccgg taaccccgct tattaaaagc attctgtaac aaagcgggac
    68341 caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa aagtccacat
    68401 tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc
    68461 ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc cgtttttttg
    68521 gatggagtga aacgatggcg attgcaattg gcctcgattt tggcagtgat tctgtgcgag
    68581 ctttggcggt ggactgcgcc agcggtgaag agatcgccac cagcgtagag tggtatcccc
    68641 gttggcaaaa agggcaattt tgtgatgccc cgaataacca gttccgtcat catccgcgtg
    68701 actacattga gtcaatggaa gcggcactga aaaccgtgct tgcagagctt agcgtcgaac
    68761 agcgcgcagc tgtggtcggg attggcgttg acagtaccgg ctcgacgccc gcaccgattg
    68821 atgccgacgg taacgtgctg gcgctgcgcc cggagtttgc cgaaaaccca aacgcgatgt
    68881 tcgtattgtg gaaagaccac actgcggttg aagaagcgga agagattacc cgtttgtgcc
    68941 acgcgccggg caacgttgac tactcccgct acattggtgg tatttattcc agcgaatggt
    69001 tctgggcaaa aatcctgcat gtgactcgcc aggacagcgc cgtggcgcaa tctgccgcat
    69061 cgtggattga gctgtgcgac tgggtgccag ctctgctttc cggtaccacc cgcccgcagg
    69121 atattcgtcg cggacgttgc agcgccgggc ataaatctct gtggcacgaa agctggggcg
    69181 gcttgccgcc agccagtttc tttgatgagc tggacccgat cctcaatcgc catttgcctt
    69241 ccccgctgtt cactgacacc tggactgccg atattccggt gggcacctta tgcccggaat
    69301 gggcgcagcg tctcggcctg cctgaaagcg tggtgatttc cggcggcgcg tttgactgcc
    69361 atatgggcgc agttggcgca ggcgcacagc ctaacgcact ggtaaaagtt atcggtactt
    69421 ccacctgcga cattctgatt gccgacaaac agagcgttgg cgagcgggca gttaaaggta
    69481 tttgcggtca ggttgatggc agcgtggtgc ctggatttat cggtctggaa gcaggccaat
    69541 cggcgtttgg tgatatctac gcctggttcg gtcgcgtact cagctggccg ctggaacagc
    69601 ttgccgccca gcatccggaa ctgaaagcgc aaatcaacgc cagccagaaa caactgcttc
    69661 cggcgctgac cgaagcatgg gccaaaaatc cgtctctgga tcacctgccg gtggtgctcg
    69721 actggtttaa cggtcgtcgc acaccgaacg ctaaccaacg cctgaaaggg gtgattaccg
    69781 atcttaacct cgctaccgac gctccgctgc tgttcggcgg tttgattgct gccaccgcct
    69841 ttggcgcacg cgcaatcatg gagtgcttta ccgatcaggg gatcgccgtc aataacgtga
    69901 tggcgctggg cggcatcgcg cggaaaaacc aggtcattat gcaggcctgc tgcgacgtgc
    69961 tgaatcgccc gctgcaaatt gttgcctctg accagtgctg tgcgctcggt gcggcgattt
    70021 ttgctgccgt cgccgcgaaa gtgcacgcag acatcccatc agcccagcaa aaaatggcca
    70081 gtgcggtaga gaaaaccctg caaccgcgca gcgaacaggc acaacgcttt gaacagcttt
    70141 atcgccgcta tcagcaatgg gcgatgagcg ccgaacaaca ctatcttcca acttccgccc
    70201 cggcacaggc tgcccaggcc gttgcgactc tataaggaca cgataatgac gatttttgat
    70261 aattatgaag tgtggtttgt cattggcagc cagcatctgt atggcccgga aaccctgcgt
    70321 caggtcaccc aacatgccga gcacgttgtt aatgcgctga atacggaagc gaaactgccc
    70381 tgcaaactgg tgttgaaacc gctgggcacc acgccggatg aaatcaccgc tatttgccgc
    70441 gacgcgaatt acgacgatcg ttgcgctggt ctggtggtgt ggctgcacac tttctccccg
    70501 gccaaaatgt ggatcaacgg cctgaccatg ctcaacaaac cgttgctgca attccacacc
    70561 cagtttaacg cggcgctgcc gtgggacagt atcgatatgg actttatgaa cctgaaccag
    70621 actgcacatg gcggtcgcga gttcggcttc attggcgcgc gtatgcgtca gcaacatgcc
    70681 gtggttaccg gtcactggca ggataaacaa gcccatgagc gtatcggctc ctggatgcgc
    70741 caggcggtct ctaaacagga tacccgtcat ctgaaagtct gccgttttgg cgataacatg
    70801 cgtgaagtag cggtcaccga tggtgataaa gttgccgcac agatcaagtt cggtttctcc
    70861 gtcaatacct gggcggttgg cgatctggtg caggtggtga actccatcag cgacggcgat
    70921 gttaacgcgc tggtcgatga gtacgaaagc tgctacacca tgacgcctgc cacacaaatc
    70981 cacggcgaaa aacgacagaa cgtgctggaa gcggcgcgta ttgagctggg gatgaagcgt
    71041 ttcctggaac aaggtggctt ccacgcgttc accaccacct ttgaagattt gcacggtctg
    71101 aaacagcttc caggtctggc cgtacagcgt ctgatgcagc agggttacgg ctttgcgggc
    71161 gaaggcgact ggaaaaccgc cgccctgctt cgcatcatga aggtgatgtc aaccggtctg
    71221 cagggcggca cctcctttat ggaggactac acctatcact tcgagaaagg taatgacctg
    71281 gtgctcggct cccatatgct ggaagtctgc ccgtcgattg ccgtagaaga gaaaccgatc
    71341 ctcgacgttc agcatctcgg tattggtggt aaggacgatc ctgcccgact gatcttcaat
    71401 acccaaaccg gtccagcgat tgtcgccagc ctgattgatc tcggcgatcg ttaccgtctg
    71461 ctggttaact gtatcgacac ggtgaaaaca ccgcactccc tgccgaaact gccggtggcg
    71521 aatgcgctgt ggaaagcgca accggatctg ccaactgctt ccgaagcgtg gatccttgct
    71581 ggtggcgcgc accataccgt cttcagccat gcgctgaacc tcaacgatat gcgccagttc
    71641 gccgagatgc acgacattga aatcacggtg attgataacg acacccgcct gccagcgttt
    71701 aaagacgcac tgcgctggaa cgaagtgtat tacggatttc gtcgctaagt agccacatca
    71761 ggtatgtaac gcctgatgcg acgctgacgc gttttatctg gcctacacgc tgcgattttg
    71821 taggccggat aagcaaagcg catccggcat tgttttcccc ctcgcccctt tggggagagg
    71881 gccggggtga ggggaaacga cctctcagga gtcaacatgt tagaagatct caaacgccag
    71941 gtattagaag ccaacctggc gctgccaaaa cacaacctgg tcacgctcac atggggcaac
    72001 gtcagcgccg ttgatcgcga gcgcggcgtc tttgtgatca aaccttccgg cgtcgattac
    72061 agcgtcatga ccgctgacga tatggtcgtg gttagcatcg aaaccggtga agtggttgaa
    72121 ggtacgaaaa agccctcctc cgacacgcca actcaccggc tgctctatca ggcattcccc
    72181 tccattggcg gcattgtgca tacgcactcg cgccacgcca ccatctgggc gcagtcgggt
    72241 cagtcgattc cagcaatcgg caccacccac gccgactatt tctacggcac cattccctgc
    72301 acccgcaaaa tgaccgacgc agaaatcaac ggcgaatatg agtgggaaac cggtaacgtc
    72361 atcgtagaaa cctttgaaaa acagggtatc gatgcagcgc aaatgcccgg cgttctggtc
    72421 cattcccacg gcccgtttgc atggggcaaa aatgccgaag atgcggtgca taacgccatc
    72481 gtgctggaag aggtcgctta tatggggata ttctgccgtc agttagcgcc gcagttaccg
    72541 gatatgcagc aaacgctgct ggataaacac tatctgcgta agcatggcgc gaaggcatat
    72601 tacgggcagt aatgactgta taaaaccaca gccaatcaaa cgaaaccagg ctatactcaa
    72661 gcctggtttt ttgatggatt ttcagcgtgg cgcaggcagg ttttatctta acccgacact
    72721 ggcgggacac cccgcaaggg acagaagtct ccttctggct ggcgacggac aacgggccgt
    72781 tgcaggttac gcttgcaccg caagagtccg tggcgtttat tcccgccgat caggttcccc
    72841 gcgctcagca tattttgcag ggtgaacaag gctttcgcct gacaccgctg gcgttaaagg
    72901 attttcaccg ccagccggtg tatggccttt actgtcgcgc ccatcgccaa ttgatgaatt
    72961 acgaaaagcg cctgcgtgaa ggtggcgtta ccgtctacga ggccgatgtg cgcccgccag
    73021 aacgctatct gatggagcgg tttatcacct caccggtgtg ggtcgagggt gatatgcaca
    73081 atggcactat cgttaatgcc cgtctgaaac cgcatcccga ctatcgtccg ccgctcaagt
    73141 gggtttctat agatattgaa accacccgcc acggtgagct gtactgcatc ggcctggaag
    73201 gctgcgggca gcgcatcgtt tatatgctgg ggccggagaa tggcgacgcc tcctcgcttg
    73261 atttcgaact ggaatacgtc gccagccgcc cgctgctgct ggaaaaactc aacgcctggt
    73321 ttgcaaacca cgatcccgat gtgatcatcg gctggaacgt ggtgcagttc gatctgcgaa
    73381 tgctgcaaaa acatgccgag cgttaccgtc ttccgctgcg tcttgggcgc gataatagcg
    73441 agctggagtg gcgcgagcac ggctttaaaa acggcgtctt ttttgcccag gctaaaggtc
    73501 ggctaattat cgacggtatc gaggcgctga aatccgcgtt ctggaatttc tcttcattct
    73561 cgctggaaac tgtcgctcag gagctattag gcgaaggaaa atctatcgat aacccgtggg
    73621 atcgaatgga cgaaattgac cgccgtttcg ccgaagataa acctgcgctg gcaacttata
    73681 acctgaaaga ttgcgagctg gtgacgcaga tcttccacaa aactgaaatc atgccatttt
    73741 tactcgaacg agcaacggtg aacggcctgc cggtggatcg gcacggcggt tcggtggcag
    73801 cgtttggtca tctctatttt ccgcgaatgc atcgcgctgg ttatgtcgcg cctaatctcg
    73861 gcgaagtgcc accgcacgcc agccctggcg gctacgtgat ggattcacgg ccagggcttt
    73921 atgattcagt gctggtgctg gactataaaa gcctgtaccc gtcgatcatc cgcacctttc
    73981 tgattgatcc cgtcgggctg gttgaaggca tggcgcaacc tgatccagag cacagtaccg
    74041 aaggttttct cgatgcctgg ttctcacggg agaaacattg cctgccggag attgtgacta
    74101 acatctggca cgggcgcgat gaagccaaac gtcagggtaa taagccgctt tcgcaggcgc
    74161 tgaagatcat tatgaatgcc ttttatggcg tgctcggcac caccgcctgc cgcttcttcg
    74221 atccgcgcct ggcatcgtcg atcaccatgc gtggtcatca gatcatgcgg cagaccaaag
    74281 cattgattga agcgcagggc tacgacgtta tctacggaga taccgactcg acttttgtct
    74341 ggctaaaagg tgcacattcg gaagaagagg cggcgaaaat cggtcgtgta ctggtgcagc
    74401 acgttaacgc ctggtgggcg gaaacgctgc aaaaacaacg gctgaccagc gcattagaac
    74461 tggagtatga aactcatttc tgccgttttc tgatgccaac cattcgcgga gccgataccg
    74521 gcagtaaaaa gcgctacgcc gggctgattc aggagggcga taaacagcgg atggtgttta
    74581 aagggctgga aaccgtgcgc accgactgga cgccgctggc ccagcagttt cagcaggagc
    74641 tatacctgcg catcttccgc aacgagccat atcaggaata tgtacgcgaa accatcgaca
    74701 aactgatggc gggtgaactg gatgcgcgac tggtttaccg taaacgcctt cgccgtccgc
    74761 tgagcgagta tcagcgtaat gtgccgcctc atgtacgcgc cgctcgcctt gccgatgaag
    74821 aaaaccaaaa gcgcggtcgc cccttgcaat atcagaatcg cggcaccatt aagtacgtat
    74881 ggaccaccaa cggcccggag ccgctggact accaacgttc accactggat tacgaacact
    74941 atctgacccg ccagctacaa cccgtggcgg agggaatact cccttttatt gaggataatt
    75001 ttgctacact tatgaccggg caacttgggc tattttgagc aaaaaaaaga gttcgccaga
    75061 taccattttg atgcgtgacg aatgctttgc catccagtac catagcgccc tttccattcc
    75121 tggacctgaa taacaccact acctcataag cacggtagcg ggtggttatt gcctgcaatt
    75181 aaagatatag agccgaacac atatgccttt tacacttggt caacgctgga tcagcgatac
    75241 agaaagcgaa ttgggacttg gaaccgttgt cgcggtggat gcgcgaactg tcactttact
    75301 tttcccatct actggtgaaa accgtctgta cgcacgcagt gattcccccg tgacccgcgt
    75361 gatgttcaac cctggtgata ctattaccag ccatgacggc tggcagatgc aagtcgaaga
    75421 agtaaaagaa gaaaatggct tgctgaccta tatcggtact cgcctggata ctgaagagtc
    75481 cggcgtagcc ctgcgtgaag ttttccttga tagcaaactg gtgttcagca aaccgcagga
    75541 ccgtctgttt gccgggcaga ttgaccgtat ggaccgcttt gcgctgcgtt atcgcgcgcg
    75601 taagtattcc agcgaacagt tccgtatgcc gtacagcggc ctgcgcggtc agcgtaccag
    75661 cctgattccg catcagctca acatcgctca tgatgttggt cgccgccacg cgccgcgcgt
    75721 cctgctggct gacgaagtgg gtttagggaa aaccattgaa gccgggatga tcctgcatca
    75781 gcaactgctc tctggcgctg ctgaacgtgt gctaattatc gtcccggaaa ccttacagca
    75841 tcagtggctg gtagaaatgc tgcgccgttt caacctgcgc tttgcgctat ttgatgatga
    75901 gcgttatgcc gaagctcagc acgatgctta caacccgttt gacaccgaac agctggtgat
    75961 ttgctcgctg gattttgccc gtcgtagcaa acagcgcctg gaacatctct gtgaagccga
    76021 atgggacctg ctggtggtcg atgaagcgca tcacctggtg tggagcgaag atgcgccaag
    76081 ccgtgaatat caggccattg aacaactggc agagcacgtg ccgggcgttc tgctgctgac
    76141 cgcgaccccg gaacagctgg ggatggaaag ccacttcgcc cgtctgcgtc tgctggaccc
    76201 gaaccgtttc cacgattttg cgcagttcgt tgaagagcag aaaaattatc gtccggttgc
    76261 ggacgccgtt gccatgctgc tggcaggtaa caaactgagc aatgacgaac tgaacatgct
    76321 cggcgagatg atcggcgagc aggatatcga gccgctgttg caggcagcaa acagcgacag
    76381 cgaagatgcc cagagcgccc gtcaggagct ggtttcgatg ctgatggatc gccacggcac
    76441 cagccgcgtg ctgttccgta acacgcgtaa cggtgtgaaa ggattcccga aacgcgagct
    76501 gcacaccatt aaactgccgc taccgacgca gtatcagacg gctattaaag tctccggcat
    76561 tatgggcgca cgtaaaagtg cggaagatcg tgctcgcgat atgctctacc cggagcgtat
    76621 ttatcaggaa tttgaaggtg ataacgccac ctggtggaac ttcgatccgc gcgttgagtg
    76681 gctgatgggc tacctgacca gccatcgctc tcagaaagtg ctggtgatct gcgccaaagc
    76741 tgccactgcg ctgcaactgg agcaggtact gcgcgaacgt gaaggtattc gcgctgcggt
    76801 gttccacgaa ggtatgtcga ttatcgaacg tgaccgcgct gccgcctggt ttgccgaaga
    76861 agacaccggc gcacaggtac tgctgtgctc agaaatcggt tctgaaggac gtaacttcca
    76921 gttcgccagc cacatggtga tgtttgacct gccattcaac ccggatctac tggagcagcg
    76981 tattggtcgt ctggatcgta tcggccaggc gcacgatatt cagatccatg tgccttatct
    77041 ggagaaaacc gctcagtcgg tgctggtgcg ctggtatcac gaaggtctgg atgcatttga
    77101 gcacacctgc ccgaccggac gcactattta cgatagcgta tacaacgatc tgattaacta
    77161 tctggcttca ccggatcaaa ccgaaggctt tgacgatctg atcaaaaact gccgcgagca
    77221 acatgaagcg ctgaaagcac agctggaaca gggtcgtgac cgcctgctgg aaatccactc
    77281 caacggtggc gaaaaagccc aggcactggc agaaagcatt gaagagcagg atgacgatac
    77341 caacctgatc gccttcgcca tgaacctgtt cgatattatc ggtatcaatc aggacgatcg
    77401 cggcgacaac atgatcgtgc tgacgccgtc cgatcatatg ctggtgccgg acttccctgg
    77461 cctgtcggaa gatggcatca ccatcacctt tgatcgtgaa gtggcactgg cgcgtgaaga
    77521 tgcacagttt attacctggg agcatccgct gatccgcaac ggtctggatc tgatcctttc
    77581 tggcgatacc ggtagcagca cgatttcact gttaaaaaac aaagcgttgc cggtaggtac
    77641 gctgttggtg gaactgattt atgtggttga agcccaggct ccgaagcagt tgcagctcaa
    77701 ccgcttcctg ccaccgacgc cggtacgtat gctgctggat aaaaacggca acaacctggc
    77761 ggcgcaggta gagtttgaaa cctttaaccg ccagcttaac gcggttaacc gtcacaccgg
    77821 cagcaaactg gttaacgccg tgcagcagga tgttcacgct atccttcaac tgggtgaagc
    77881 gcagatcgag aaatctgccc gtgcattgat tgatgcagcg cgtaacgaag ccgacgaaaa
    77941 actgtctgcc gagctgtctc gtctggaagc tctgcgtgca gtgaacccga acattcgtga
    78001 cgacgaactg accgccattg agagcaaccg tcagcagata atggaaagcc tggatcaggc
    78061 aggttggcgt ctggatgccc tgcgtttgat cgttgtgacg catcagtaac ggagctgaaa
    78121 atggggatgg aaaactacaa tccaccgcag gaaccctggt tggttatcct gtatcaggat
    78181 gaccacatta tggtggtcaa caagccgagc ggtttgttgt ctgtgccggg tcgtctggaa
    78241 gagcacaaag acagcgtgat gacgcgcatt cagcgtgatt atccgcaggc agaatcggtg
    78301 catcgtctgg atatggctac cagcggcgtg attgtagtgg cgctgaccaa agccgcggag
    78361 cgggagttaa aacgccagtt ccgcgagcgc gagccgaaaa agcagtatgt ggcccgcgtc
    78421 tgggggcatc catcccccgc agaaggtctg gtggatctgc cgctgatttg cgactggcca
    78481 aaccgcccga aacagaaagt ctgttacgaa acgggtaaac ctgcgcagac ggaatatgaa
    78541 gtggtggagt atgcggcgga taacacggca agagtggtgt taaaaccgat taccgggcgt
    78601 tcgcatcaat tgcgtgtgca tatgctggcg ctggggcatc cgattctcgg cgatcgtttt
    78661 tatgcatcac cagaagcgag agcgatggca ccgcgtttgt tgctacatgc agagatgttg
    78721 acgattaccc atccggcgta tggcaatagt atgacgttta aagcgccagc ggatttttaa
    78781 tcgtcacgct ggcgcagttc ggtaggcgga taaggcgttc acgccgcatc cggcaattgt
    78841 gcgccgattg cctgatgcga cgcgggccgc gtcttatcag gcctacagtt taaaacttat
    78901 ttaaaccctt tctgctgctt tatcagctca tatgcctgct gaatttcctg cgctttctgc
    78961 ttcgccatct ccatcatctc aggcggcaaa cctttcgcca ccagcttatc gggatggtgt
    79021 tcactcatca gcttacggta ggcacgtttg atggtggtcg catcatccgt cggcttcacg
    79081 cccagcacat tacaggcatc ttccagcgtc gggccacgct gcgcttgctg ccagttacca
    79141 ccgccagatt gctgctgata accgccgcca aactgtgcac cgccctgcat catgcgcaaa
    79201 aactggtcaa actgggcgcg ggagatccct aattcttcag caatgacata cagcaccgcc
    79261 cgttcattcg ggtgcagtga accatcagca aacgccgcct gaatctggat ctccagaaac
    79321 atacgaatta agtcaaaacg accaaagcag acactgcgaa actggcgcat cttttcgcgc
    79381 agcgggtaat tgtctgattt tcccacccgg aacgcatttt gcgccgcagt acgggaagcg
    79441 ccatgaagat tcattcggtc catcaactgg ctagcgatat gaatatcagc ctccgtgacg
    79501 cgacctttgg atttggttaa atgccccatc acttcaaaag tggtggcaaa aaacagcgcc
    79561 tgacgctcac gctggttggc gaaccacgcc attttacggc tacgggcttt atcaaacata
    79621 tggccaatta acaggcctaa cactacgccc caaaagccgc cgcccatcag taaggccacg
    79681 gccacgccaa tgatttttcc ccaatactgc atatattccc caaatcgaca cacggatatc
    79741 agggctatct cccacaatat aaaggtgctt ttaccgtttt ccggctgcgg tcaattgtgg
    79801 gacatcgcct ataatttgca ttatcatacc tgtcattcaa tgccgtgcct aacaccactg
    79861 acgctattcg gacaggatta acactagcgt agagatgacg agtacgttag tctctgaccg
    79921 tttgtcacgc gcaacgttac cgatgatgga acaataaaat caacgtatga aaaaacgtat
    79981 ccccactctc ctggccacca tgattgccac cgccctttat agtcaacagg gactggcagc
    80041 cgacctcgcc tcacagtgca tgttgggcgt gccaagctat gaccgtcctc tggtacaggg
    80101 cgataccaat gacttacccg tgactatcaa tgctgaccac gcgaaagggg actacccgga
    80161 tgacgccgtg tttactggca gcgtggatat catgcagggt aacagccgtc tgcaggccga
    80221 cgaagtgcag ctccatcaaa aagaggcacc aggacaaccg gagccggtac gtaccgttga
    80281 tgcgctcggt aatgtccatt acgacgataa ccaggtgatc ctcaaagggc cgaaaggctg
    80341 ggcgaatctg aacaccaaag ataccaacgt ctgggaaggt gattaccaga tggtgggtcg
    80401 ccagggtcgc ggtaaagcgg acctgatgaa acaacgtggc gaaaaccgct ataccattct
    80461 ggataacggt agctttacct cctgtctgcc gggttctgac acctggagcg tggtaggtag
    80521 cgaaattatt catgaccgcg aagaacaagt tgcggagatc tggaacgccc gctttaaggt
    80581 gggtccggta ccgatctttt atagccccta tttgcagttg ccggtgggtg acaaacgtcg
    80641 ctctggtttc ttgatcccga acgccaagta caccaccacc aactactttg agttctacct
    80701 gccatattac tggaacatcg cgccaaatat ggatgccacc atcacgccgc attatatgca
    80761 tcgtcgtggc aacatcatgt gggagaacga attccgctac ctctcccaag cgggcgctgg
    80821 cttgatggaa ctggactatc tgccttcaga taaagtctat gaagatgaac acccgaacga
    80881 tgacagttca cgtcgttggt tattctactg gaaccactcc ggggtcatgg atcaggtgtg
    80941 gcgtttcaac gtcgactaca ccaaggtcag cgatcctagc tacttcaatg atttcgataa
    81001 caagtacggt tccagtactg acggctacgc aacgcaaaaa ttcagcgttg gctatgcggt
    81061 gcaaaacttc aatgccaccg tttcaaccaa gcagttccag gttttcagcg aacagaacac
    81121 cagtagctac tcggcagagc cgcagttaga cgttaattac taccagaatg atgttggtcc
    81181 gtttgatacg cgtatttacg gccaggcagt gcactttgtt aacaccagag acgacatgcc
    81241 tgaagcaacc cgtgttcacc tggaaccgac catcaatttg ccgctctcta ataactgggg
    81301 cagcatcaat accgaagcga agttgctggc aacccattat cagcaaacca atcttgactg
    81361 gtataactcc agaaacacga ccaagctgga cgaatccgtt aaccgcgtaa tgccgcaatt
    81421 caaagttgac ggcaaaatgg tctttgaacg cgatatggaa atgctggctc cgggttatac
    81481 ccaaacgctg gaaccgcgcg cgcagtattt gtacgtgccg tatcgcgatc agagcgacat
    81541 ctataactac gactcgtctc tgctgcaatc tgactactct ggcctgttcc gggaccggac
    81601 ttacggcggt cttgaccgta ttgcctccgc taaccaggtg acgaccggtg tcacatctcg
    81661 catatatgat gatgctgccg ttgaacgttt taatatttcc gttggtcaaa tctactattt
    81721 cacggagtct cgcactggcg atgacaacat aacatgggag aatgacgaca aaacgggttc
    81781 actggtgtgg gcaggcgata cttactggcg tatctccgag cgttggggat tgcgtggcgg
    81841 gattcagtac gatacacgtc tggataacgt agcgaccagt aactccagca ttgaataccg
    81901 tcgggatgaa gaccgtctgg tacagctgaa ttaccgttac gccagcccgg aatatattca
    81961 ggctacgctg cctaagtact attccactgc tgagcaatat aagaatggta tttcgcaggt
    82021 aggtgctgtc gccagctggc caattgccga tcgttggtcc attgttgggg cctactacta
    82081 cgacaccaat gcgaacaagc aagccgactc tatgttaggt gtgcaataca gctcctgctg
    82141 ctatgcaatt cgcgtcggtt acgagcggaa gctgaacggt tgggataacg ataaacaaca
    82201 tgcggtatat gacaacgcaa tcggctttaa catcgaactt cgcggcctga gctccaacta
    82261 cggtctgggt acgcaagaga tgctgcgttc gaacattctg ccgtatcaaa acactttgtg
    82321 atttgttgat ttaccacgta atccgcagtg cggttaattg aaatggaaaa agtatgaaga
    82381 actggaaaac gctgcttctc ggtatcgcca tgatcgcgaa taccagtttc gctgcccccc
    82441 aggtagtcga taaagtcgca gccgtcgtca ataacggcgt cgtgctggaa agcgacgttg
    82501 atggattaat gcagtcggta aaactgaacg ctgctcaggc aaggcagcaa cttcctgatg
    82561 acgcgacgct gcgccaccaa atcatggaac gtttgatcat ggatcaaatc atcctgcaga
    82621 tggggcagaa aatgggagtg aaaatctccg atgagcagct ggatcaggcg attgctaaca
    82681 tcgcgaaaca gaacaacatg acgctggatc agatgcgcag ccgtctggct tacgatggtc
    82741 tgaactacaa cacttatcgt aaccagatcc gcaaagagat gattatctct gaagtgcgta
    82801 acaacgaggt gcgtcgtcgc atcaccatcc tgccgcagga agtcgaatcc ctggcgcagc
    82861 aggtgggtaa ccaaaacgac gccagcactg agctgaacct gagccacatc ctgatcccgc
    82921 tgccggaaaa cccgacctct gatcaggtga acgaagcgga aagccaggcg cgtgccattg
    82981 tcgatcaggc gcgtaacggc gctgatttcg gtaagttggc gattgctcat tctgccgacc
    83041 agcaggcgct gaacggcggc cagatgggct ggggccgtat tcaggagttg ccggggatct
    83101 tcgcccaggc attaagcacc gcgaagaaag gcgacattgt tggcccgatt cgttccggcg
    83161 ttggcttcca tattctgaaa gttaacgacc tgcgcggcga aagcaaaaat atctcggtga
    83221 ccgaagttca tgctcgccat attctgctga aaccgtcgcc gatcatgact gacgaacagg
    83281 cacgcgtgaa actggaacag attgctgctg atatcaagag tggtaaaacg acttttgctg
    83341 ccgcagcgaa agagttctct caggatccag gctccgctaa ccagggcggt gatctcggct
    83401 gggctacacc agatattttc gatccggcct tccgtgacgc cctgactcgc ctgaacaaag
    83461 gtcaaatgag tgcaccggtt cactcttcat tcggctggca tttaatcgaa ctgctggata
    83521 cccgtaatgt cgataaaacc gacgctgcgc agaaagatcg tgcataccgc atgctgatga
    83581 accgtaagtt ctcggaagaa gcagcaagct ggatgcagga acaacgtgcc agcgcctacg
    83641 ttaaaatcct gagcaactaa tggttaaaac ccaacgtgtt gtgatcactc ccggcgagcc
    83701 cgccgggatt ggcccggact tagttgtcca gcttgcacag cgtgagtggc cggtcgaact
    83761 ggttgtttgt gccgatacca ctctccttac cgaccgggca gcgatgctcg gtttgccgct
    83821 caccctccgc ccttattccc ccaactcccc tgcacaaccg caaactacgg gcacattaac
    83881 gctacttcct gtcgcgctac gtgaatctgt cactgcgggg cagttagggg ttgaaaatgg
    83941 gcattacgtg gtggagacgc tggcgcgagc gtgtgatggc tgtctgaacg gtgaatttgc
    84001 tgcgctgatc acaggccccg tgcataaagg cgtcattaac gacgcaggta ttccgtttac
    84061 tggtcatacc gagtttttcg aagagcgttc gcaggcgaaa aaagtggtga tgatgctggc
    84121 gaccgaagaa cttcgcgtgg cgctggcaac gacgcattta ccgctgcgcg atatcgcaga
    84181 tgctatcacc cctgcacttt tgcacgaagt gattgctatt ttgcatcacg atttgcggac
    84241 aaaatttggt attgccgaac cgcgcattct ggtctgcgga ctgaatccgc acgcgggcga
    84301 aggcggtcat atgggtacgg aagagataga caccattatt ccggtgctcg acgagctgcg
    84361 ggcgcagggg atgaaactca acgggccgct gcctgccgat accctgtttc agccgaaata
    84421 tctcgataac gccgacgccg tgctggcgat gtaccacgat cagggtcttc ccgtgctaaa
    84481 ataccagggc ttcgggcgcg gtgtgaacat tacgctgggc ctgcccttta ttcgcacatc
    84541 agtggaccac ggcaccgcgc ttgaactggc gggacgtggc gaagccgatg tcggcagttt
    84601 tattacggcg cttaatctcg ccatcaaaat gattgttaac acccaatgaa taatcgagtc
    84661 caccagggcc acttagcccg taaacgcttc gggcaaaact ttctcaacga tcagttcgtg
    84721 atcgacagca ttgtttctgc cattaacccg caaaagggcc aggcaatggt cgaaatcggc
    84781 cccggtctgg cggcattgac cgagccggtc ggcgaacgtc tggaccagct gacggtcatc
    84841 gaacttgacc gcgatctggc ggcacgtctg caaacgcatc cattcttagg cccgaaactg
    84901 acgatttatc agcaggatgc gatgaccttt aactttggcg aactggccga gaaaatgggt
    84961 cagccgctgc gtgttttcgg caacctgcct tataacatct ccacgccgtt gatgttccat
    85021 ctgtttagct atactgatgc cattgccgac atgcacttta tgttgcaaaa agaggtggtg
    85081 aatcgtctgg ttgcaggacc gaacagcaaa gcgtatggtc gattaagcgt catggcgcaa
    85141 tactattgca acgtgatccc ggtactggaa gttccgccgt cagcctttac accaccaccc
    85201 aaagtggatt ccgccgtcgt gcgcctggtt cctcatgcaa cgatgcctca cccggttaaa
    85261 gatgttcgtg tgttgagccg catcaccacc gaagccttta accagcgtcg taaaaccatt
    85321 cgtaacagcc tcggcaacct gtttagcgtc gaggtgttaa cgggaatggg gatcgacccg
    85381 gcgatgcgag cggaaaatat ctctgtcgcg caatattgcc agatggcgaa ctatctggcg
    85441 gagaacgcgc ctttgcagga gagttaacga tgatcaattc gccccgagtg tgtattcagg
    85501 ttcaaagcgt ctacattgag gcacaatctt cacctgataa tgaacgttac gtttttgctt
    85561 ataccgtaac catacgcaat ctggggcgag cgccagtgca gttgttgggg cgttactggc
    85621 tgatcaccaa tggcaatggc cgtgaaaccg aagtccaggg cgaaggagtg gttggcgtcc
    85681 agccacttat cgcgcctggc gaagagtacc agtacaccag cggtgcaatc attgaaaccc
    85741 cgctgggcac catgcagggt cactacgaaa tgatcgatga aaatggcgtc cctttcagca
    85801 tcgacattcc cgtattccga ctcgccgttc ccacactcat tcattaaaag aatatggcga
    85861 cataccttat tggcgacgtt catggttgtt acgatgaact gatcgcattg ctgcataaag
    85921 tagaatttac ccctgggaaa gataccctct ggctgacggg cgatctggtc gcgcgcgggc
    85981 cgggttcgct ggatgttctg cgctatgtga aatccttagg cgacagcgta cgtctggtgc
    86041 tgggcaatca cgatctgcat ctgctggcgg tatttgccgg gatcagccgc aataaaccga
    86101 aagatcgcct gacaccgctg ctggaagcgc cggatgccga cgagctgctt aactggctgc
    86161 gtcgccagcc tttgctgcaa atcgacgaag agaaaaagtt ggtgatggcc cacgcaggga
    86221 tcacgccgca gtgggatcta cagaccgcca aagagtgcgc gcgcgatgta gaagcggtgc
    86281 tatcgagtga ctcctatccc ttctttctcg acgctatgta cggcgatatg ccaaataact
    86341 ggtcaccgga attgcggggg ctgggaagac tgcgttttat caccaacgcc tttacccgta
    86401 tgcgtttttg ctttccaaac ggtcaactgg atatgtacag caaagaatcg ccggaagaag
    86461 cccctgcccc actgaaaccg tggtttgcga ttcctggccc tgtcgctgaa gaatacagca
    86521 tcgcctttgg tcactgggca tcgctggaag gcaaaggtac gccggaaggt atttacgcgc
    86581 tggataccgg ctgctgctgg ggtgggtcat taacctgcct gcgctgggaa gataagcagt
    86641 attttgtcca gccgtcgaac cggcataagg atttgggcga agcggcggcg tcttaaacac
    86701 agccagatat aggaaggccg gataagacgc gaccggcgtc gcatccggca ctagccgtaa
    86761 attctataca aaattaccgc cgctccagaa tctcaaaaca atagctgtga gagttctgcg
    86821 catcggcgtc gtggaattcg ctgaataccg attcccagtc atccggctcg taatccggga
    86881 aatgggtgtc gccttccact tctgcgtcga tatgcgtcag atacagtttt tgcgcttttg
    86941 gcaggaactg ctcataaacg cgaccaccgc caatcaccat gatttctggt acgtcaccac
    87001 acgccgcgat ggcttcatcc accgatttca cccacgttac gcgatcgtcc gtacccggtt
    87061 gactgctgag gataatattt ttgcgtcctg gcaacggacg accgatggat tcccaggtat
    87121 ggcggcccat aatcacgggt ttatttaagg tgttgcgttt aaaccaggcg agatcggcag
    87181 gcaggttcca cggcatggcg ttttccatgc cgataacacg atctaccgct aacgccgcaa
    87241 tcagactaat cattgattta tcctggatat aaaaaattgt cgccactata cgtaaagcat
    87301 aaaccgtcgt cgactggcgc aaggatgatg ttgagtaaaa ttttacgttc tgctggcgag
    87361 tccacgctct cccactggac tcgccacatt acaatgaaac aaaaacaaac agttagctgt
    87421 aaagtgtgat ttacgtcact ctttattagg atgagggttt cgtttccggt tcatccgcca
    87481 tgttgccggt atgtttccct tcttcggttc cctgccagcc atgtcgttga attaatgaca
    87541 gatgttcgcg gtcttcggta atgatctcac ttaacatcgc gctggtgcgt ttatagaccg
    87601 ctgcgcgggc tttggtgtcg ttctcaacca ttgccatctc ttccaccatc tgaatattaa
    87661 agcggcggaa cacatcggca cgttctcgcg cttcatacgg ccccagacct aaactttcca
    87721 gtgccagacg cccggttttc agcgcacctt cgaaggtttc acgctccggc ttttcaacgc
    87781 ctgcctgacg caaacgaatg tagtgatcga catcgcgggc gcgggcaata atctgcaaat
    87841 gcgggaaatg ttctttcacc atctccgtca gttgcaggtt ggtttgcgga tcgtcaattg
    87901 cgttaatcag cacttccgct ttcgccgctc cggcagactc cagtaaatcc atccgcgtgg
    87961 catcgccata aaacactttc ataccaaatt tacgcaaggt ttcgatatgg tccgggtcgt
    88021 gatcgagcac caccattttc accccgctgg agagcagtaa acgtccggtg atctgcccga
    88081 aacgaccgaa tccggcgata atcacgcgcg gctgttcttc gtcgatctca tcggcttcac
    88141 gcgcttcctc agtagaagat tgctcaaggc gattgaggat caccagcaga atcggcgttg
    88201 ctgccatcga cagcgccacc gccagggtca gcgatttcgc ccactccggc tccagcacat
    88261 tcgccatctg cgccgcacca aataccacaa aggcaaactc actaccctgc cctaacaaca
    88321 ccacaaacca gcgacgctgt ttatttggca cctgcaacgg tcgggcaatc agccacagca
    88381 tggcgatttt gatgatgagg aaaccgagca gcaaaatgac aatgcgcaat gggttttcaa
    88441 tcagcgtgcc aaagtctatc gacatgccaa caccgatgaa aaacagcccc aacagcaaac
    88501 ctttaaatgg ttcgatatcg ctttccagcg catgacggta ttccgagctt gccagcagta
    88561 cacccgccag aaacgcgccc atcgccatcg acaagccaac ctcttccagc agcaaaccaa
    88621 agccaaacac gaggaataac gccacggcac taaacacttc ccgcaagcca gagcgggcta
    88681 caaaacgcag cgccggacgc gtgacatagc gccccagcaa taccaccagc accagcgccc
    88741 ccgccacttt taacgccgag agagcaaatg cgcccatcgt cgtcgaggca ctgctcgccg
    88801 ccagtagcgg gatcatcgcc accagcggga tcgccgcgat atcctggaac agcagcaccg
    88861 caaaggcact gcgacccatt tgcgtcacca tcagattgcg ttcattcatc gcctgcatgg
    88921 caatcgccgt agaggagagc gccagcgtca tgccgatcaa ttccgcgacc tgccagcgca
    88981 gcccaagtaa catgcagaac agccccagca ggccgccgca aatcaccatc tgcaatgcgc
    89041 caccgccgaa cactgccgca cgcagcttcc acagcctttg tggatcgagt tcgaggccga
    89101 taataaacag catcagcacc accccaatct cggcaaagtg cagaatagat tcggcatcgg
    89161 tcaccagtcg cagcccccac gggccaataa tgcagccagc gatcaggtag ccaagtaccg
    89221 atcccagacc aagacgtacc gcaatgggta caatcagcgc cgccgatccg aggtagatca
    89281 gcgcctgaac cagcgtatgg ctatccatga tgggcctcct gccattccag cagacgctgc
    89341 ttatagtgac gcgcctgccc ttcgagggtt tcgtcgtcac aaataaaggt acagtgcatg
    89401 gcaaacggtg gcagccagtt cagcccgcag tagattgccg tcgcctgtag cggctgcgac
    89461 agcacatcaa aacccggatg cgcaccaatt tcaaaatggc tttccccgcc gccggtcgtc
    89521 accgcccaca gcaaatgttt gccatgcagc gccgtgccgc catgaccgta agcccagccg
    89581 tgggagaaaa ctttatcgat ccaaagtttg aggagcggag gaacgctgta ccactgcatc
    89641 gggtgctgcc agacaatcag atcggcgcga gacagcgcct cctgctcggc ggcaatatcg
    89701 atattgaagt caggatagag ttgataaaga gagcgaattt cgacgccttc cagcgtcctt
    89761 gcctgttcaa gcatccgttt attcgcatgg gaatgatgcg gatacggatg cgcataaatt
    89821 ataagaatca taggtagccc gcgtgattac gcctttgttt tcccacaaag tgtagtcata
    89881 tattcacgcg gcttatagtc aataatattg attcagaaaa gagagaaatc tgaacaaatt
    89941 accccattct ctgcccgcgg atcgtcatat tgctggtctg cgccagtgac atccctcgag
    90001 tctccggggc aaacgctacg gaaatcaaca agccaaacag cgagataccc gcccccatta
    90061 gcatcgtgtt actgataccg taattattga taaagatcgg cagtgcccag gtcgaaacaa
    90121 tggtgccaat acgacttaag gacataatca cgcccacggc agaggcgcgg atatccgtcg
    90181 ggaagagttc attaggatag agccactgca aattacccgg cccgccagag aaaaaggcat
    90241 acaccgcaaa ggccatcact accagccaga tccccatatc cgggatcagc cccaaaaccg
    90301 ccagcgccag cgtcatcatg gcaaagctgc caatcaacaa tggacgccgt ccggcagtgt
    90361 ttagccacag catcggcgga atacagccga gcataaagaa caggctaatc accacattcc
    90421 ccagtgccgc gtttttgcca acccccaatc ccaacaaacc aacgatttgc gggccaaagg
    90481 tgtaaatggc gaacattggg atcacctggc aggtccagat ggcggcaaca aacagaacaa
    90541 aaggaaaata gcggcgatta aacagatcgc gaaaacgggt ttgctgcggc tgctcttcat
    90601 cgaaagccac cggttcgcca aacagtttga tcatcatctc ttcgcactct tttactcgcc
    90661 ctttgcgtaa cagccagcgg ggagattcag gcagttcgaa tcgaccaatc aaaatcaaca
    90721 aacaggggat cgccgcgcta cccagcatcc agcgccagcc gccttccaca tcataaagcc
    90781 agtagccgac cagatcggcg caggtcgcgc cgacatacca catggcggcg ataaagctga
    90841 tggaaaatgc ccgctgacgg gtactggaga actcggtgat cattgaggtg gcaatgggat
    90901 aatctgcacc gatgacaatg ccgataagta cccgcatcac caacagttcg acgggggatg
    90961 aaacaaacat cgtcgccacc gatatcacgc cgatggcgat gatatcaatg aggaacattt
    91021 tgcgccgtcc gactttatcg gagatatagc caaacagcga tgtgccaacg aacagcccgg
    91081 cgagcgttcc cgcgcccagc aagccaatcc agtcagcgtc cagtttcagc gccggggtca
    91141 gttgctccag cgccacgcca atcattacca gtacataacc atccagaaac ggaccaccgc
    91201 ttccccacag caaaatgcgg cggtgaatag aggagaattt gagatcgtca aagtttctgg
    91261 acggttgcat gggttattcc tgttttttgc aatgagagtc agtgcggcgt tacgtatcaa
    91321 accaacatca gccgtaacgg aactccacac caaaggtgcc gcgcgggtat tcccactgtt
    91381 ccagcgccga ccccagcccc agaatgcgac aggtgccgca ctccagacat ccggcgtaat
    91441 cgaagcgcac actgccgtca tcctgcttct tgtacagacc tgcggggcac gctttcacca
    91501 gcagctccag cgcctgttta tcagcatcgg ccttcacaac aatgtgcgga tgctcttcat
    91561 cgacattgaa tttattgacg cccagtttga cgtccacatt gacgggagaa gtcataaaac
    91621 ggtcactcct ttcatgccat ccttgattag attgatgaag cccactttct taccgtggcg
    91681 gaggattttc ttgcgcatca gttctggcgc actgccgtca atggtgaaca ggtcacgcgc
    91741 cacgcccacc gccagttccg ggtagccgct aaacatgcgt gggttatcaa ggaacgccgg
    91801 tagtttctga tacatacgca tatcgcgcag cggaccactc tcaagatgct gacgatattc
    91861 cgccagtttt tgcttactga aatcgtcgct tttcatcgct gaaagcacgg tttttgctgc
    91921 ggcttccccg gcggcaatcg ccagatccat accgcgaatg gtaaaaccga gattcataca
    91981 cattccggcg gcatcaccgg caatcaatac gccgtcacca accaactccg gcagcatgtt
    92041 gatgcctgct tccggcacta cgtgagcgga atattccacc agcttgccac ctgcgatcag
    92101 tggtgcaacg gccggatgtt gtttgaaatc ttccagcatt tgcggcaccg attttttcgc
    92161 gtcatgcaga tgatgcaaac cacaaaccag ccccagcgac agggtgtttt cattggtata
    92221 aagaaaaccg ccgcccatca ggccatcggt gggtgatccc gcaaacaggc aagccgcgcc
    92281 ctgattaccc tgcaactgaa aacggtcttc gattaccgac ttcggtaact cgatcagttc
    92341 cttcacgcca accgccacat ccgtcggttt aacgcgtttt gccatcccca gcttttcggc
    92401 aaggatggag ttcaccccat cagcaaggat caccgttttc gcttcaatca catcgccatc
    92461 ggcttctaca ccgacaactt tgccatcgcg ctgtacgagg ttatcgacgc ggatcccggt
    92521 aattaactgc gcgcccgctt cttcggcctg ctccatcagc caggcatcaa atttactgcg
    92581 caaaacggag taagaacgct gggatggcga ggtttcgtca ccattgcagt agtccatagt
    92641 catcgctgac ttttccgtca taaacgcgag tttttcatgg gtgatcaggc gttctaccgg
    92701 ggcagattcg gcgaaacctg gaataatgtg ttccaggctg tgggcataga gacgcccgcc
    92761 ggtgacgttc ttggcacctg cggaattgcc acgctcgata actaacactt gcgcaccttc
    92821 gcgggcgagc accagtgcgg caaccgaacc ggcaagccct gcaccgacga tgatggcgtc
    92881 aaagatatct tcggacataa gagctccctg tcagcggcag gaccaaaatg cagccctgcc
    92941 agagtggatc aacgcgctaa agctgctgtc agcgccggaa ggatcttcac ggcgtcgcca
    93001 acaatgccgt aatccgcgta ctggaagatc ggcgcatttt tatctttatt gatggcgaaa
    93061 atggtttgcg atgcattagc accaaccatg tgctggatct gcccggagat ccccaccgcc
    93121 aggtacagtt caggtttcag catcaggttg gagataccga catagcgttc gtgctccatc
    93181 catttttcgt tttccgccac cggacgagaa caggccaact ccgcacctat cgccttacaa
    93241 agctgttctg ccagcgcaat gttatctttg ctgccaatgc cgcgaccgac gctgaccacc
    93301 agacgggctt tgtcgagatc gacgctgttg ctctggcgcg cctgggttgc cgtgcgggta
    93361 atcgccacag ccggagcctg ccactccatg gtgtgcgttt cgccagtgcg tgacgcgtct
    93421 ggctgagccg catcgaacat gccactgctg atggtcagta ccgcatacgg cgtggcaatg
    93481 cgttcttcgc caatcgccag gccgccgtaa accatgtgtt tcactgtcgc tttaccgtcc
    93541 tgtacgctga cggtgctggc atcgttagac accgccgctt taaggcgata acccagtttt
    93601 gccgccagta atttgccgcg acgggtattt ggcaacagca ccagaccgtc tgcgccgtgc
    93661 tggcgaatag tgtcagccat gacaccggcg taatcttcga tcatccgatc gtccggtttg
    93721 ccgtttaatt tccagacatg attagcgccg agctggattg cctgtgcgcc gtcggcatca
    93781 ttgaggacaa aggtgttgat ttgattagct aaagcctgcg caccgttcat cagttccggc
    93841 agacgagaag gggtatcgct gaatacccag acttgagaaa acgtgttcat agcatcccct
    93901 gtaattaaat gactttgcga agattttcag caaatgcggc gatctgttct tcgccgtcgc
    93961 cttcaatcac gatgcgctga cgttcgcgct gtttcggcgc ggcaacctgt tgttcagacc
    94021 aggcgtccac ggcgttaaaa ccgatatccg ccgccgacca tacctggact ggctttttcg
    94081 ccgcgccgag aatggctttc atcgaaggaa tttgtgggga gttgatatca gtggaaacag
    94141 caataaccgc aggcagcgga atgcttaacg tttctgtttc atcttccagt tcgcgctcaa
    94201 cggtgagggt atctgccgtc agggagataa ttttgctgac gccgttaact gccggaatat
    94261 tgaggatttc gcctaccagc aaaccaacct gctgggcata aaggtcggaa gaaccatcgc
    94321 cacagaggat caaatcaaag cctgctttct gggcggcggc agccagtgcg ctcgccgttt
    94381 gttgcggcag tgcctgctcg aactggtcat caatcaccac aatcagttca tccgggccgc
    94441 gcgatagcac atctttacgc cctttggcgt tggtcagggc tttaccgccc acacttaagg
    94501 ccgtcacctg cgcctctgct gcctgttgct ttagctggca agccgcttca atagcgttca
    94561 gatcgtattg gcttattttg gcatcggctt tgctgaagtc taatgaacca tcagcattat
    94621 tgaccgcaat atcctgttca tcaggcacgc acttatagca agtaataatc ttcattgcat
    94681 ctccagaaat catgaaggaa acgtcgttca tatcggtggt gttaattaca gatatcacgc
    94741 cctttaattt ggaaataaaa ctttaatcac caatattgaa aatgtcacac gcagataaat
    94801 ccaactttca atattgttaa gttcctcacc aatattgaaa acatggcgta gcaaaaagaa
    94861 attttcaata ttgctttatg gatcaccaat attgaaagtc agatgctatt tttttactca
    94921 caacacaaca taacaaactg attaataatc aaaattaacg agaaaagcgc cctctattca
    94981 cccgaaacaa aaatgtgata acaatcacag aatgcagctt attgaat
//
LOCUS       DAZGSP010000004        79722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZGSP010000004 DAZGSP010000000
VERSION     DAZGSP010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.004"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(123..410)
                     /gene="yebO"
                     /locus_tag="ACHK74_000299"
     CDS             complement(123..410)
                     /gene="yebO"
                     /locus_tag="ACHK74_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFP0219185.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(485..628)
                     /gene="mgrB"
                     /locus_tag="ACHK74_000300"
     CDS             complement(485..628)
                     /gene="mgrB"
                     /locus_tag="ACHK74_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFP0219186.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            787..1026
                     /gene="yobH"
                     /locus_tag="ACHK74_000301"
     CDS             787..1026
                     /gene="yobH"
                     /locus_tag="ACHK74_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFP0219187.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1169..1960)
                     /gene="kdgR"
                     /locus_tag="ACHK74_000302"
     CDS             complement(1169..1960)
                     /gene="kdgR"
                     /locus_tag="ACHK74_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFP0219188.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2137..3510
                     /gene="yebQ"
                     /locus_tag="ACHK74_000303"
     CDS             2137..3510
                     /gene="yebQ"
                     /locus_tag="ACHK74_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0219189.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3555..4436)
                     /gene="htpX"
                     /locus_tag="ACHK74_000304"
     CDS             complement(3555..4436)
                     /gene="htpX"
                     /locus_tag="ACHK74_000304"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFP0219190.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4628..6676)
                     /gene="prc"
                     /locus_tag="ACHK74_000305"
     CDS             complement(4628..6676)
                     /gene="prc"
                     /locus_tag="ACHK74_000305"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFP0219191.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6696..7394)
                     /gene="proQ"
                     /locus_tag="ACHK74_000306"
     CDS             complement(6696..7394)
                     /gene="proQ"
                     /locus_tag="ACHK74_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFP0219192.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7491..7988)
                     /gene="msrC"
                     /locus_tag="ACHK74_000307"
     CDS             complement(7491..7988)
                     /gene="msrC"
                     /locus_tag="ACHK74_000307"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFP0219193.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8118..9401
                     /gene="yebS"
                     /locus_tag="ACHK74_000308"
     CDS             8118..9401
                     /gene="yebS"
                     /locus_tag="ACHK74_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFP0219194.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9370..12003
                     /gene="yebT"
                     /locus_tag="ACHK74_000309"
     CDS             9370..12003
                     /gene="yebT"
                     /locus_tag="ACHK74_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFP0219195.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVAGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSVSGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFKQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12083..13522
                     /gene="rsmF"
                     /locus_tag="ACHK74_000310"
     CDS             12083..13522
                     /gene="rsmF"
                     /locus_tag="ACHK74_000310"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFP0219196.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13640..13876
                     /gene="yebV"
                     /locus_tag="ACHK74_000311"
     CDS             13640..13876
                     /gene="yebV"
                     /locus_tag="ACHK74_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFP0219197.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13981..14172
                     /gene="yebW"
                     /locus_tag="ACHK74_000312"
     CDS             13981..14172
                     /gene="yebW"
                     /locus_tag="ACHK74_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFP0219198.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14173..14829)
                     /gene="pphA"
                     /locus_tag="ACHK74_000313"
     CDS             complement(14173..14829)
                     /gene="pphA"
                     /locus_tag="ACHK74_000313"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP0219199.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15225..15566)
                     /gene="yebY"
                     /locus_tag="ACHK74_000314"
     CDS             complement(15225..15566)
                     /gene="yebY"
                     /locus_tag="ACHK74_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFP0219200.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15579..16451)
                     /gene="copD"
                     /locus_tag="ACHK74_000315"
     CDS             complement(15579..16451)
                     /gene="copD"
                     /locus_tag="ACHK74_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFP0219201.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16455..16829)
                     /gene="yobA"
                     /locus_tag="ACHK74_000316"
     CDS             complement(16455..16829)
                     /gene="yobA"
                     /locus_tag="ACHK74_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFP0219202.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16968..17198
                     /gene="holE"
                     /locus_tag="ACHK74_000317"
     CDS             16968..17198
                     /gene="holE"
                     /locus_tag="ACHK74_000317"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFP0219203.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17300..17956
                     /gene="yobB"
                     /locus_tag="ACHK74_000318"
     CDS             17300..17956
                     /gene="yobB"
                     /locus_tag="ACHK74_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFP0219204.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17980..18642
                     /gene="exoX"
                     /locus_tag="ACHK74_000319"
     CDS             17980..18642
                     /gene="exoX"
                     /locus_tag="ACHK74_000319"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFP0219205.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18639..20699)
                     /gene="ptrB"
                     /locus_tag="ACHK74_000320"
     CDS             complement(18639..20699)
                     /gene="ptrB"
                     /locus_tag="ACHK74_000320"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFP0219206.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPESETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20908..21567)
                     /gene="yebE"
                     /locus_tag="ACHK74_000321"
     CDS             complement(20908..21567)
                     /gene="yebE"
                     /locus_tag="ACHK74_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP0219207.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(21893..22249)
                     /gene="yebF"
                     /locus_tag="ACHK74_000322"
     CDS             complement(21893..22249)
                     /gene="yebF"
                     /locus_tag="ACHK74_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFP0219208.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22316..22606)
                     /gene="yebG"
                     /locus_tag="ACHK74_000323"
     CDS             complement(22316..22606)
                     /gene="yebG"
                     /locus_tag="ACHK74_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFP0219209.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22740..23918
                     /gene="purT"
                     /locus_tag="ACHK74_000324"
     CDS             22740..23918
                     /gene="purT"
                     /locus_tag="ACHK74_000324"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFP0219210.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLSTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23974..24615)
                     /gene="kdgA"
                     /locus_tag="ACHK74_000325"
                     /gene_synonym="eda"
     CDS             complement(23974..24615)
                     /gene="kdgA"
                     /locus_tag="ACHK74_000325"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP0219211.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24652..26463)
                     /gene="edd"
                     /locus_tag="ACHK74_000326"
     CDS             complement(24652..26463)
                     /gene="edd"
                     /locus_tag="ACHK74_000326"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFP0219212.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26698..28173)
                     /gene="zwf"
                     /locus_tag="ACHK74_000327"
     CDS             complement(26698..28173)
                     /gene="zwf"
                     /locus_tag="ACHK74_000327"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFP0219213.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDKDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28511..29380
                     /gene="hexR"
                     /locus_tag="ACHK74_000328"
                     /gene_synonym="yebK"
     CDS             28511..29380
                     /gene="hexR"
                     /locus_tag="ACHK74_000328"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFP0219214.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29508..30950
                     /gene="pyk"
                     /locus_tag="ACHK74_000329"
     CDS             29508..30950
                     /gene="pyk"
                     /locus_tag="ACHK74_000329"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFP0219215.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAARLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31082..32053)
                     /gene="lpxM"
                     /locus_tag="ACHK74_000330"
                     /gene_synonym="msbB"
     CDS             complement(31082..32053)
                     /gene="lpxM"
                     /locus_tag="ACHK74_000330"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFP0219216.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32173..33495)
                     /gene="mepM"
                     /locus_tag="ACHK74_000331"
     CDS             complement(32173..33495)
                     /gene="mepM"
                     /locus_tag="ACHK74_000331"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFP0219217.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33511..34443)
                     /gene="znuA"
                     /locus_tag="ACHK74_000332"
     CDS             complement(33511..34443)
                     /gene="znuA"
                     /locus_tag="ACHK74_000332"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFP0219218.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            34522..35277
                     /gene="znuC"
                     /locus_tag="ACHK74_000333"
     CDS             34522..35277
                     /gene="znuC"
                     /locus_tag="ACHK74_000333"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFP0219219.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35274..36059
                     /gene="znuB"
                     /locus_tag="ACHK74_000334"
     CDS             35274..36059
                     /gene="znuB"
                     /locus_tag="ACHK74_000334"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFP0219220.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(36206..37216)
                     /gene="ruvB"
                     /locus_tag="ACHK74_000335"
     CDS             complement(36206..37216)
                     /gene="ruvB"
                     /locus_tag="ACHK74_000335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFP0219221.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37225..37836)
                     /gene="ruvA"
                     /locus_tag="ACHK74_000336"
     CDS             complement(37225..37836)
                     /gene="ruvA"
                     /locus_tag="ACHK74_000336"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFP0219222.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPTTDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(37975..38040)
                     /gene="yobI"
                     /locus_tag="ACHK74_000337"
     CDS             complement(37975..38040)
                     /gene="yobI"
                     /locus_tag="ACHK74_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFP0219223.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            38111..38713
                     /gene="yebB"
                     /locus_tag="ACHK74_000338"
     CDS             38111..38713
                     /gene="yebB"
                     /locus_tag="ACHK74_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFP0219224.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(38715..39236)
                     /gene="ruvC"
                     /locus_tag="ACHK74_000339"
     CDS             complement(38715..39236)
                     /gene="ruvC"
                     /locus_tag="ACHK74_000339"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFP0219225.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(39271..40011)
                     /gene="yebC"
                     /locus_tag="ACHK74_000340"
     CDS             complement(39271..40011)
                     /gene="yebC"
                     /locus_tag="ACHK74_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0219226.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(40040..40492)
                     /gene="nudB"
                     /locus_tag="ACHK74_000341"
     CDS             complement(40040..40492)
                     /gene="nudB"
                     /locus_tag="ACHK74_000341"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFP0219227.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(40610..42382)
                     /gene="aspS"
                     /locus_tag="ACHK74_000342"
     CDS             complement(40610..42382)
                     /gene="aspS"
                     /locus_tag="ACHK74_000342"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFP0219228.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            42692..43258
                     /gene="yecD"
                     /locus_tag="ACHK74_000343"
     CDS             42692..43258
                     /gene="yecD"
                     /locus_tag="ACHK74_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP0219229.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            43255..44073
                     /gene="yecE"
                     /locus_tag="ACHK74_000344"
     CDS             43255..44073
                     /gene="yecE"
                     /locus_tag="ACHK74_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFP0219230.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            44126..44521
                     /gene="yecN"
                     /locus_tag="ACHK74_000345"
     CDS             44126..44521
                     /gene="yecN"
                     /locus_tag="ACHK74_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFP0219231.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            44562..45305
                     /gene="cmoA"
                     /locus_tag="ACHK74_000346"
     CDS             44562..45305
                     /gene="cmoA"
                     /locus_tag="ACHK74_000346"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFP0219232.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            45302..46273
                     /gene="cmoB"
                     /locus_tag="ACHK74_000347"
     CDS             45302..46273
                     /gene="cmoB"
                     /locus_tag="ACHK74_000347"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFP0219233.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(46438..48867)
                     /gene="torZ"
                     /locus_tag="ACHK74_000348"
     CDS             complement(46438..48867)
                     /gene="torZ"
                     /locus_tag="ACHK74_000348"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFP0219234.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTTRIQHPMVRKSYLDNPLQPT
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTIGYPQFEEYLRGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNVFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(48892..49992)
                     /gene="torY"
                     /locus_tag="ACHK74_000349"
     CDS             complement(48892..49992)
                     /gene="torY"
                     /locus_tag="ACHK74_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFP0219235.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(50380..51126)
                     /gene="cutC"
                     /locus_tag="ACHK74_000350"
     CDS             complement(50380..51126)
                     /gene="cutC"
                     /locus_tag="ACHK74_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFP0219236.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(51140..51706)
                     /gene="yecM"
                     /locus_tag="ACHK74_000351"
     CDS             complement(51140..51706)
                     /gene="yecM"
                     /locus_tag="ACHK74_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP0219237.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            51922..53655
                     /gene="argS"
                     /locus_tag="ACHK74_000352"
     CDS             51922..53655
                     /gene="argS"
                     /locus_tag="ACHK74_000352"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFP0219238.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(53697..53741)
                     /gene="yecV"
                     /locus_tag="ACHK74_000353"
     CDS             complement(53697..53741)
                     /gene="yecV"
                     /locus_tag="ACHK74_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFP0219239.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            53832..54320
                     /gene="yecT"
                     /locus_tag="ACHK74_000354"
     CDS             53832..54320
                     /gene="yecT"
                     /locus_tag="ACHK74_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFP0219240.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYHNSEDFYPSPLT
                     RG"
     gene            complement(54440..54832)
                     /gene="flhe"
                     /locus_tag="ACHK74_000355"
     CDS             complement(54440..54832)
                     /gene="flhe"
                     /locus_tag="ACHK74_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFP0219241.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLWVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(54832..56910)
                     /gene="flhA"
                     /locus_tag="ACHK74_000356"
     CDS             complement(54832..56910)
                     /gene="flhA"
                     /locus_tag="ACHK74_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFP0219242.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(56903..58051)
                     /gene="flhB"
                     /locus_tag="ACHK74_000357"
     CDS             complement(56903..58051)
                     /gene="flhB"
                     /locus_tag="ACHK74_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFP0219243.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(58253..58897)
                     /gene="cheZ"
                     /locus_tag="ACHK74_000358"
     CDS             complement(58253..58897)
                     /gene="cheZ"
                     /locus_tag="ACHK74_000358"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFP0219244.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(58908..59297)
                     /gene="cheY"
                     /locus_tag="ACHK74_000359"
     CDS             complement(58908..59297)
                     /gene="cheY"
                     /locus_tag="ACHK74_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFP0219245.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(59312..60361)
                     /gene="cheB"
                     /locus_tag="ACHK74_000360"
     CDS             complement(59312..60361)
                     /gene="cheB"
                     /locus_tag="ACHK74_000360"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFP0219246.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(60364..61224)
                     /gene="cheR"
                     /locus_tag="ACHK74_000361"
     CDS             complement(60364..61224)
                     /gene="cheR"
                     /locus_tag="ACHK74_000361"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFP0219247.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(61243..62844)
                     /gene="tap"
                     /locus_tag="ACHK74_000362"
     CDS             complement(61243..62844)
                     /gene="tap"
                     /locus_tag="ACHK74_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFP0219248.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQESQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(62890..64551)
                     /gene="tar"
                     /locus_tag="ACHK74_000363"
     CDS             complement(62890..64551)
                     /gene="tar"
                     /locus_tag="ACHK74_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFP0219249.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(64696..65199)
                     /gene="cheW"
                     /locus_tag="ACHK74_000364"
     CDS             complement(64696..65199)
                     /gene="cheW"
                     /locus_tag="ACHK74_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFP0219250.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(65220..67184)
                     /gene="cheA"
                     /locus_tag="ACHK74_000365"
     CDS             complement(65220..67184)
                     /gene="cheA"
                     /locus_tag="ACHK74_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFP0219251.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVALEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(67189..68115)
                     /gene="motB"
                     /locus_tag="ACHK74_000366"
     CDS             complement(67189..68115)
                     /gene="motB"
                     /locus_tag="ACHK74_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFP0219252.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FKTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(68112..68999)
                     /gene="motA"
                     /locus_tag="ACHK74_000367"
     CDS             complement(68112..68999)
                     /gene="motA"
                     /locus_tag="ACHK74_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFP0219253.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFVSYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(69126..69704)
                     /gene="flhC"
                     /locus_tag="ACHK74_000368"
     CDS             complement(69126..69704)
                     /gene="flhC"
                     /locus_tag="ACHK74_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFP0219254.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(69707..70057)
                     /gene="flhD"
                     /locus_tag="ACHK74_000369"
     CDS             complement(69707..70057)
                     /gene="flhD"
                     /locus_tag="ACHK74_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFP0219255.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            70837..71265
                     /gene="uspC"
                     /locus_tag="ACHK74_000370"
     CDS             70837..71265
                     /gene="uspC"
                     /locus_tag="ACHK74_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFP0219256.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGDISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(71272..72696)
                     /gene="otsA"
                     /locus_tag="ACHK74_000371"
     CDS             complement(71272..72696)
                     /gene="otsA"
                     /locus_tag="ACHK74_000371"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFP0219257.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(72671..73471)
                     /gene="otsB"
                     /locus_tag="ACHK74_000372"
     CDS             complement(72671..73471)
                     /gene="otsB"
                     /locus_tag="ACHK74_000372"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFP0219258.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(73638..74624)
                     /gene="araH"
                     /locus_tag="ACHK74_000373"
     CDS             complement(73638..74624)
                     /gene="araH"
                     /locus_tag="ACHK74_000373"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFP0219259.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(74639..76153)
                     /gene="araG"
                     /locus_tag="ACHK74_000374"
     CDS             complement(74639..76153)
                     /gene="araG"
                     /locus_tag="ACHK74_000374"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFP0219260.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARHKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(76223..77212)
                     /gene="araF"
                     /locus_tag="ACHK74_000375"
     CDS             complement(76223..77212)
                     /gene="araF"
                     /locus_tag="ACHK74_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFP0219261.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            78009..78512
                     /gene="ftnB"
                     /locus_tag="ACHK74_000376"
     CDS             78009..78512
                     /gene="ftnB"
                     /locus_tag="ACHK74_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFP0219262.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(78591..78842)
                     /gene="yecJ"
                     /locus_tag="ACHK74_000377"
     CDS             complement(78591..78842)
                     /gene="yecJ"
                     /locus_tag="ACHK74_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFP0219263.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(78957..79043)
                     /gene="azuC"
                     /locus_tag="ACHK74_000378"
     CDS             complement(78957..79043)
                     /gene="azuC"
                     /locus_tag="ACHK74_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFP0219264.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            79306..79629
                     /gene="yecR"
                     /locus_tag="ACHK74_000379"
     CDS             79306..79629
                     /gene="yecR"
                     /locus_tag="ACHK74_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFP0219265.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT        19210 a        20918 c        19322 g        20272 t
ORIGIN      
        1 atgataatta tttgaagtca gaccagagtt ttaatttgaa gcagttgcac catggcagtg
       61 cgtataatca ggatgaattg agtaaccctt caacgacggg gttactcaag attgaggaag
      121 gattatcgtt cggcgaccag acgaataata tcttcgtctt caaccgattt ttgactctca
      181 acggtctttt tatctgcttt ctcaggttcg tttgcttcgc acaaacgacg taacagtgca
      241 ttttgacgtt tttgctgatc cagcaacgcc tcaagcagtt caatctgttc gttagtccga
      301 gaactggcac gattgataaa aaaccacaag atgagcccga taagaagaac caccaccgat
      361 acaaccaaag acgcaatgtt catcacgcct gaatttacaa cttcgttcat ttcaccacct
      421 ctgagtagag gcgctattct accactgctg gagaggaaga aaatctagtg ctgaaaaaat
      481 gatatcacca cgggataaac tggttaatgg cacaaattcc gctgaaaaat tgtacatcct
      541 gatcgcacat catgttgaat acctgcgccc aaagcagcaa gcaagccaac accacgacaa
      601 ccagaacgac ccatcgaaac tttttcactc caatctccgc ttcacctacc ttatgtcatt
      661 aaactagcat gcgttagtta aacagcgcct aactatgtcg atattcgtgc tttttcggaa
      721 tgagtagctt gcttcagcgc atattgccgc tacgattaag cgaacataaa aagagaagag
      781 gttgtaatgc gattcatcat tcgcacagtt atgctgatag cactggtatg gataggatta
      841 ttactcagcg gatatggcgt tttgattgga agcaaagaga atgccgcagg attaggtttg
      901 caatgtacct atctgactgc cagaggcacc agcacggtac aatacttgca cactaaaagt
      961 ggtttcttgg ggataacaga ttgcccactg ttacgtaaaa gcaatatcgt ggtcgataat
     1021 ggttaagggt aataaataac cagattctca ctcatgagta aaatattctt atagcaagcg
     1081 cgtttaatat aacgcacctt caactttatt tttctcatgc taaataaaga acctgtctgt
     1141 ttgatattcc gacaggttct tactctcatc agaacggata gtcgtgataa cccatttggg
     1201 cagaaatttt gcgcgctgcg gtatgcaaca ttgcgacata ttcctgtaaa cgctcttcag
     1261 agaaacgcaa cgtcgggaag gagatgctca aaccggcaat gaccacgcca aagcgatcaa
     1321 ataccggtac cgcaatgcat cgcagccctt cttcctgctc ttcgttatct tcgccatacc
     1381 cctgctcgcg cacctggtcc agaacgggta ataacgcttc tgtactggtg atggtccgct
     1441 cggtactgcg tttatactcc acgccctcaa gaatttgctt cacttcatcg cgatcgcgcc
     1501 atgccagcag taccttacca atcgcggtgc tgtacagcgg attacgacgc ccaatccgtg
     1561 aatacatgcg caaattgtac atagagtcaa ttttgtgaat gtaaacaata ctgtcttcgt
     1621 ccagtgcgcc gaggtggata gtttctttgg tcaggcggga gatctcacgc atctggatat
     1681 ctgcgctacg aattaaatcg acgttttgta acgcgcgagc gcccagttca aacaatttca
     1741 gggtcaggga atatttctcc gactcccctt cctgcgccac ataacctaag gttttcatgg
     1801 tctgtaaaaa gcgataaacg gtgctttttg acatcatgac gcgctgcgac agctcggtta
     1861 tccctatttc gcgctcttca cccagcgcct gcaaaatgcc aaaaactttt agcacggaag
     1921 atacagaatc aggctgttta tccagatctg cgttagccat ttatcacctc attgcgagtg
     1981 ttttataaaa atcagaactg ttttttatta taatttcgca ccagggtggt cgcaatccat
     2041 cttttgccgg ttagttacaa ttctgcgaca tccaccgtga atatcagtgc tagaatcata
     2101 cccctgttga ttattcacca aagatataaa attcctatgc caaaagttca ggccgacggc
     2161 ctgccattgc cccagcgata cggtgcgata ttaaccattg tgattggtat ttcgatggct
     2221 gtccttgacg gcgcaatcgc caacgtcgcc ctgccaacaa tcgccacgga ccttcatgcc
     2281 acgccagcca gttccatctg ggtagtgaac gcctatcaaa tcgccattgt catctccctg
     2341 ctctcgtttt cgtttctggg cgatatgttt ggctatcgac gtatttataa atgcggtctg
     2401 gtcgtttttc tgttgtcttc actgttctgc gccctttctg attcgctgca aatgctcacc
     2461 cttgcgcgtg tcatacaagg tttcggcggt gcagcgttga tgagcgttaa taccgcactt
     2521 atccgcctga tctatccaca acgttttctg gggagaggga tgggcataaa ctcgtttatt
     2581 gttgccgtct cttctgctgc cgggccgaca attgctgcag caatcctctc catcgcatcc
     2641 tggaaatggt tatttttaat caacgtaccg ttaggtatta tcgccctgct tctggcgatg
     2701 cgttttctgc cacccaatgg ttctcgcgcc agtaaacccc gtttcgacct gcccagcgcc
     2761 gtgatgaacg cgttaacctt cggcctgctt atcactgcgt tgagtggttt cgctcagggg
     2821 caatcgctga cgttaattgc tgcggaactg gtggtaatgg ttgttgttgg tattttcttt
     2881 attcgccgcc agctttctct tcccgtaccg ctgctaccgg tggatttact gcgtatcccg
     2941 ctgttttcac tttctatttg cacatctgtt tgctctttct gcgcacaaat gctggcaatg
     3001 gtttccctgc ccttttacct gcaaaccgtg ctcgggcgta gtgaagtcga aacaggttta
     3061 cttctgacac cgtggccgtt agcaacgatg gtgatggctc cgctggcagg ctatttgatt
     3121 gaacgcgtac atgcaggatt gctgggtgct ttagggttgt tcatcatggc tgcggggctt
     3181 ttttccctgg ttctgctgcc cgcgtcacct gcggatatca atattatctg gccgatgatc
     3241 ttatgtggcg ctggatttgg cttattccag tcacccaata accacaccat tattacctcc
     3301 gctccgcgcg aacgtagcgg tggggccagt ggcatgttag gaacggctcg tctactgggt
     3361 cagagtagtg gcgcggcgct ggtggcgctg atgctaaatc agttcggtga taatggtacg
     3421 cacgtttcgc tgatggctgc ggctattctg gcagtgattg ctgcctgtgt gagtggttta
     3481 cgtatcactc agccacgatc cagggcataa aaaaagcgcg tcgacaggac gcgcttttta
     3541 gtatttactt catattactt caggtattca cccgtacgca gagcttcaat tcgtttatcc
     3601 aacggcgggt gagtcatgaa caactcactg agcgatttcg acttaccgtt aatgcagaaa
     3661 gccatcatgc tggttgcttc ttgcggttca tagctggttt tcaggcgctg cagcgcggca
     3721 atcattttct cgcgaccaac cagttttgcc gaaccggcat cagcatggaa ttcacgatga
     3781 cgcgagaacc acatggtgat aatgctcgcc agaataccaa acaccagttc cagaaccgtt
     3841 gcaaccgcaa agtagatcag cgggttgccg ttgctctctt caccttcatc acgatttccg
     3901 cccataaaac ccgcggcaag ctgcgccaga atacgggaaa taaagataac gaaggtgttc
     3961 accacgccct gaatcagcgt catggtaacc atatcaccat tggcgatgtg gctgatttcg
     4021 tgagcaatta ccgcctcggc ttcatccggg ctcatgttct gcagcaaacc ggtgctgaca
     4081 gcaaccagag aggcatcacg gcgcgcaccg gttgcaaaag cgttgatgtc cggcgcatgg
     4141 tagatagcca cttgcggcat agcgatccct gcctgacgag cctgggttgc tacagtatta
     4201 accagccaac gttccctttc gttacgcggt tgctcgatca cttccccgcc aacagatcgt
     4261 aatgccatcc atttggacat cagaagcgaa acgaaggaac caccaaaacc gaacagcaag
     4321 gccatgatca tcagcccctg aacgctgctc gactgtatcc ctgtcaggct cagtaccagc
     4381 ccgaaaacga ccattacggc caggttcgtt agcaggaaga gcgcgattcg catcataatt
     4441 ttctttttac ctcagtttaa caaaacgcaa tatgcgatac ccacatcgta tgggttacgc
     4501 gactattttc aagtctggat agtgcgtaag tcaccagaaa gacacaactt tacattttgt
     4561 agcatctgat ttacggcatc ttgccgctgt taaaaaatca ggcacaattt cttgtgcctg
     4621 attgatatta cttgacggga gcgggttgtt ccgcgggtct ggctttttca agcttcgcca
     4681 gatcgagtgc gatattcacc gtctcatcca gataaggatc cggctcctgg taatctttcg
     4741 gtagatcatc cagcttcttc aactccggtt taccttcgcg tttaaagcgt tcgttcaaac
     4801 gcgccagacg cgtcgcatca tcttcattat tctctttctc acgcacagcg tagttcagag
     4861 aaacgatatt gcgcttgtcc ttcatagcgt tgaagcgcgc gatatccttc atgatgttct
     4921 ggaactcagg atctttcgcg atacgcgcat tatgttcctt cagcagctcc ggcccaaagg
     4981 ccgttaaatc gcctgatttc acataagtcg cggcatcaat gctatcccac ggcagcgcgt
     5041 tatcttcgaa tttctcaccc gtttccgttt cttcattacc cgtcggcatg atgatgtctg
     5101 gcgttacgcc tttacgttgc gtactgccgc cgttaacgcg atagaatttc tggatcgtgt
     5161 actgcacaga acccagcgct ggccattcag gacgtaacat ctgatcgtaa atacggttca
     5221 atgaacggta ttgctgaacg gtgcctttac caaacgtcgg ttcacccaca accagcgcac
     5281 gaccgtaatc ctgcattgcc gcggcaaaga tttctgaagc cgaagcactg aaacggtcaa
     5341 ccagtaccac cagcgggcct ttatagaaca cctgcccgtc ggtatcgctg tcttcacgaa
     5401 ccttgccgtt gttatcgcgg acctgaacaa tgggacccgc aggaataaac agacctgaga
     5461 gcgataccgc ttcggtcaac gccccaccgc cattgctacg caggtcgatg atcacactgc
     5521 tgacattctg tttttccagt ttctgcagtt gaactttgac atcgtctgtc aaacccacat
     5581 agaagcccgg aatatccagc acgccgactt tctctttacc gacggtcttc accgacattt
     5641 taaccgcgcg gtcttcgaga cgaatacgtt cacgggtcaa tgttacagtg cgggtcttgg
     5701 tccctttacc agcaggtaaa atttccagac gaactttact gcccttcggc cctttaatta
     5761 aggcaaccac atcatcaaga cgccagccaa tcacgtcaac catcggcttg cctgtttgac
     5821 caacaccgac aattttgtca ccaacgctga tagctttact cttcgctgcc ggaccacctg
     5881 ccaccatcga attgataacg gtgtagtcat catccatttg cagcactgcg ccaatacctt
     5941 ccagcgacaa actcatttca gtgttgaact gttcggtatt acgcggggaa agatagttgg
     6001 tatgcgggtc gatttcacgc gcaaacgccg tcattgccag cgagaaaaca tcttcgctgt
     6061 tggtttgcgc cagacgacga atggcaaatt tgtagcggcg tgtcagggtt tcacgaattt
     6121 ctttatccgt ttttcctgcc agcttcaggc ttaactcgtc gaatttgact ttactgtccc
     6181 acagcgcgtt caactcagcc tcgtttttcg gccagggcgc tttgctgcgg tcaaggttat
     6241 aagtgtcgtt gccggtgaaa tccatcggct tttccagtac cgacaaagcg tactggtaac
     6301 gctcaaaacg gcgcttttgc gccagattgt agagatcgta gaaaacgtcg agtttgcctg
     6361 aacgcagttc atcgcctaac tcggttttct ttttcgcgaa ctgttcaaca tcgcttgcca
     6421 gcagcacgtt gtggctgtaa tcgagcagat tcaggtagcg gtcaaagatt ttggccgaaa
     6481 atgcctgatc gaggtcgaac tggcgataat gagaacgggt gaagcgcgac gttacgcgct
     6541 cacttaccgt cgcatgctgc gtctcttcct ttaataccgg aatttgatca gcacgcgtga
     6601 tatcttctac agcgaaggtc tggcctgcta ttgcaagcag gccagctaac gcggtaagcc
     6661 taaaaaacat gttcatgcct ggcccggcct ccgtttcaga acaccaggtg ttctgcgcgc
     6721 acaatcaaag acatacccga attcagctgg acgcggacgc cgtctttggt gatttctaat
     6781 acggtggcat ccatcgcgtt ttgacccgct ttcaccttca gggcttgtcc gacagtcaga
     6841 gctgaaatgt cagaaaccgg ggtgtgctgt tcttcgcgag gtgcttttac tgtttttggc
     6901 gctttctcta ccggcttttg cgcacgaggt ttacgttcag cgccttcttt gcggcgtggc
     6961 gtggtcggac gtggcttgcg ttcgcggcgc ggtgcgtctt ctttctcacc agcagctgcg
     7021 gcagcttcgc gttttttcgc ttgctgttca gcacgctgtg cctgaacacg cgctttcgct
     7081 tcttcaagct gcttgcgagc atgctctaca tgttgctcgt ccagctcacc gcatgggttg
     7141 ccgtcaagat cgacacgcgt tgcgccgggt ttaacaccgt aaagataacg ccagctcgaa
     7201 gtgtagagac gtaaagcgga tcgtaattgc gttttgctca ggttcatttc cccggcaaca
     7261 cgatcgacca aatcctgaaa aataccgatt ttcagcggac gcgcttcacc ttccgcactg
     7321 aaacagtggg gaaaacgttc ggccagaaac gcgattactt ctttactgct attcaactta
     7381 ggttgatttt ccatgaaatt tcctgattac aacggacgta gccaacaagc gcaggcatga
     7441 acaggcgtca ttataatgac gctaccagta aatgctacgt tatccgttga ttatcctgcg
     7501 acgctcgcaa agaatttttt gtaatccgtc gttgcaagca ctttttcaag ctgtgccaca
     7561 agctgacgta agccttgctc gtcctcgtct gtaaagcgac cgaagacggt actatcgatg
     7621 tcaagaacac caataatctg atttttcacc accagcggca gaacaatttc agaattactc
     7681 gccgcatcac aggcaatatg cccgtcaaac gcgtgaacat cctcgatacg ctgcacttga
     7741 ttgcgggcaa ccgcagtgcc gcacaccccg cgcccgacgg gtatccggac acaggcaatt
     7801 ttgccctgaa atggtcctag taccagtgta tcgtcctcaa gcaaataaaa acctgcccag
     7861 tttacgtcag tgagacgctc gtataacaac gcactggtgt tcgcaagcgt tgccagaaaa
     7921 ctggtttctc ccgccatcag cgcgttaaag tcgcgattta aatccgcgta aaattctgtt
     7981 ttgttcatta tataatcact tggttgtctt acctggaact gccagcctat taaaataagc
     8041 attaaatgcg ttaatgctca agatcattcc aatcatgggt taatattaat gttaattctt
     8101 attacatttg gcacgtcatg gctcttaaca caccacaaat tacgccgaca aaaaagataa
     8161 cagtgagggc aatcggcgag gaactgccgc gtggtgatta ccaacgttgc ccgcaatgtg
     8221 acatgctgtt tagcctgccc gagataaatt ctcatcagag tgcctattgt ccgcgctgtc
     8281 aggcaaaaat tcgcgacggg cgcgactggt cgctaacgcg cctggcggca atggctttca
     8341 ccatgctgtt gctgatgccg tttgcctggg gcgaaccgct gttgcatatc tggctgttag
     8401 gtattcgtat cgacgccaac gtcatgcaag gcatctggca aatgaccaaa cagggcgata
     8461 cgataacggg ggcgatggtc tttttctgcg ttattggtgc ccccctcatt ctggtgtcct
     8521 ccatagcata tttatggttt ggtaaccgac tgggaatgaa tttacgtccg gtactgctga
     8581 tgcttgagcg tcttaaagag tgggtaatgc tggatatcta cctggtcggc attggcgttg
     8641 cttctataaa ggtacaggat tatgcccata tccaggctgg tgtcggcttg ttctcttttg
     8701 tggcgttggt gattttaacg acggtgacgt tgtcacatct taatgttgag gagctgtggg
     8761 agcgatatta tccgcagcgc cccgctacgc gtagggacga gaaacttcgt gtctgtcttg
     8821 ggtgccattt taccggctat cctgatcagc gtggtcgctg cccgcgttgc catatcccgc
     8881 tacgcctgcg tcgccgtcat agtctgcaaa aatgctgggc ggcgctgtta gcgtcaatcg
     8941 ttttgttgtt accagccaac ctattgccta tttctatcat ttatctgaac ggaggacggc
     9001 aggaagatac aattctttcc ggaattatgt cgctggcaag tagcaacatt gcggttgcgg
     9061 gaatcgtgtt tatcgccagt attctggtac cgtttactaa agtgatcgtc atgttcactt
     9121 tactgttgag cattcatttt aaatgccagc aaggtttacg cacacgcatt ctgttactgc
     9181 ggatggtgac ctggattgga cgctggtcga tgctcgacct gtttgtcata tctttaacca
     9241 tgtcgctgat taatcgcgat cagatcctcg cttttactat gggaccggct gcgttttatt
     9301 ttggcgcagc cgtaattttg actattcttg ctgtggaatg gctggacagc cgcttacttt
     9361 gggatgcaca tgagtcagga aacgcccgct tcgacgactg aagcgcagat taaaaataaa
     9421 cgccgtatct cacctttctg gctgctgcct ttcatcgcgc taatgattgc cggttggctg
     9481 atttgggaca gttatcagga ccggggtaat accgtcacca tcgactttat gtcggcggat
     9541 ggtattgttc cgggccgtac gcctgttcgt tatcagggcg ttgaagtcgg aacagtgcag
     9601 gatatcagcc tcagcgacga tcttcgtaag attgaagtca aggtcagcat caagtccgat
     9661 atgaaagatg cgctgcgcga agagactcaa ttctggctgg tgacgccaaa agcatcgttg
     9721 gcaggtgtct ccgggctgga cgccctcgtc gctgggaact atatcggcat gatgccgggt
     9781 aaaggtaaag agcaggatca ctttgtcgca ctcgataccc aaccgaagta tcggctggat
     9841 aacggcgatc tgatgatcca cctgcaagct cccgatctcg gttcgctgaa cagcggttca
     9901 ttggtctatt tccgcaagat cccggtggga aaagtctacg actatgccat caatcccaac
     9961 aagcaaggcg tggtgattga tgtcctgatc gagcggcgtt ttaccgatct ggtgaaaaaa
    10021 ggtagccgtt tctggaacgt ttccggcgtt gatgccaacg tcagtgtcag tggcgcgaag
    10081 gtgaaactgg aaagtctggc ggcactggtt aacggtgcga ttgccttcga ttcacctgaa
    10141 gagtcgaaac ctgccgaggc ggaagatacc tttggtctgt atgaagatct ggcccacagc
    10201 cagcgtggcg taataataaa actggaactg ccgagtgggg ccggattgac cgccgactcg
    10261 acgccgttaa tgtatcaggg gctggaagtc ggacagctaa ctaaactgga tttaaatcct
    10321 ggtggtaaag tcactgggga aatgacagtt gatcccagcg tcgttaccct gcttcgtgaa
    10381 aatacccgca tcgaattacg taatccgaaa ttatccctta gcgatgccaa tctcagcgcc
    10441 ctgctgactg gcaaaacctt cgagttggta cccggcgatg gcgagccacg caaagagttc
    10501 gttgttgtgc caggcgaaaa agcactgctg catgaacctg atgttctgac gctgaccctg
    10561 accgcgccgg aaagttacgg tattgatgct ggtcagccgc tcattcttca cggcgtgcag
    10621 gtaggccagg ttatcgatcg taaactcacc agcaaaggcg tcacctttac cgtcgccatc
    10681 gagcctcagc atcgagaact ggtaaaaggc gatagcaaat ttgtcgtcaa cagccgtgtc
    10741 gacgtgaagg tggggctgga tggcgttgag tttctcggtg ccagcgcctc agaatggatt
    10801 aacggcggga tacgtattct gccgggcgat aaaggcgaga tgaaagccag ctatccactg
    10861 tatgccaatc tggaaaaagc gctggagaac agccttagcg atttacccac cacaaccgtg
    10921 agtttgagtg cagagacgct gccggatgtg caggcaggat cggtagtgct ctaccgtaaa
    10981 tttgaagttg gtgaagttat taccgtccgt ccgcgagcta acgcgtttga tatcgatctg
    11041 catattaagc cggagtatcg caaccttctg accagcaata gcgtgttctg ggcagaaggc
    11101 ggggcgaaag ttcagctgaa tggtagtggc ctgaccgtac aggcatcccc gctctccaga
    11161 gcattaaagg gagccattag cttcgataac ctcagcggtg ccagcgccag tcagcgtaaa
    11221 ggcgacaaac gaattctgta tgcttccgaa acagcggccc gtgcggttgg tgggcagatt
    11281 acgcttcacg ctttcgatgc cggaaaactg gcggtcggga tgccaattcg ctatctcggt
    11341 attgatatcg ggcaaatcca gacgctggat ctgattaccg cgcgcaatga agtacaggca
    11401 aaggcggtgc tctatccgga gtatgtccag accttcgccc gcggcggtac gcgcttctcg
    11461 gtggtcacac cgcaaatttc agccgcgggc gttgagcatc tagatactat cctccagccg
    11521 tatatcaacg tcgaaccagg ccggggcaat cctcgccgcg actttgaatt acaagaggcc
    11581 accattactg attcgcgtta cctggatggc ttaagcatta ttgttgaagc gccggaagcc
    11641 ggttcgttag gtattggtac gcctgtgctg ttccgtggtc tggaagtcgg tacggttaca
    11701 ggaatgacgc tggggacatt gtcagatcgc gtgatgattg cgatgcgcat cagtaaacgc
    11761 tatcaacacc tggtgcgtaa caattccgtc ttctggttgg catcgggtta cagtctggac
    11821 tttggtctga cgggcggcgt agtgaaaacc ggcaccttta agcagtttat ccgtggcggc
    11881 atcgccttcg ccacgcctcc gggtacgcca ctggcaccga aagcccagga aggcaaacac
    11941 ttcctgttgc aggaaagtga accgaaagag tggcgtgaat ggggaactgc gcttcccaaa
    12001 taatgcccac tgctccggcg tgcctgcgcc ggagcgttta tgctaaactg cgcgcctgtt
    12061 tttttgccag tggtacatgc tcgtggccca acacaccgtt tatttcccgg acgcctttct
    12121 gacacaaatg cgcgaagcga tgccttcgac gctctcattt gatgattttc ttgccgcctg
    12181 tcagcgcccg ttgcgccgca gcattcgcgt taatacgctg aaaatctccg ttgctgattt
    12241 cctgcaatta accgctcctt atggctggac gcttacgcca attccgtggt gtgaagaagg
    12301 tttctggatt gaacgcgaca atgaagatgc attgccattg ggtagtaccg ccgagcattt
    12361 aagcggcctg ttttatattc aggaagccag ttcaatgttg cccgttgccg ccttgtttgc
    12421 tgacgataat gcaccacagc gggtgatgga tgtcgcagct gcgccaggct ccaaaacgac
    12481 gcaaattgcc gcgcggatga ataacgaagg ggcaatcctt gccaatgagt tttccgccag
    12541 tcgggtaaaa gtgttacatg ccaatatcag ccgctgtggc atcagtaatg ttgcgctcac
    12601 acattttgat ggccgcgtgt ttggtgcggc agtgccagaa atgttcgatg ccattttgct
    12661 ggacgctccc tgctctggcg aaggcgtggt gcgtaaagat cccgatgcgc taaaaaactg
    12721 gtcaccagaa agcaatcagg aaatcgcagc tacacaacgg gagcttatcg acagcgcctt
    12781 tcatgcatta cgtcctggtg gtacgctggt ttactcgacc tgtaccttaa accaggaaga
    12841 aaacgaagcc gtttgcctgt ggctgaaaga gacttacccc gacgcagtag agtttttacc
    12901 acttggcgat ctcttccctg gtgcaaacaa agcgctgacc gaagaaggct ttttgcatgt
    12961 tttcccacaa atttacgact gcgaaggctt cttcgttgct cgtctgcgta aaactcaggc
    13021 gatccccgcc ttacccgccc ccaaatacaa agtgggcaat ttcccgttca gcccggtgaa
    13081 agatcgcgaa gccggacaaa ttcgtcaggc ggctgcaagt gttggcttaa actgggatgg
    13141 aaacctgcga ctctggcaac gcgacaaaga actgtggttg ttcccggtgg gcattgaagc
    13201 cctgatcggt aaagtccgat tttctcgctt ggggattaaa cttgccgaaa cgcacaacaa
    13261 aggttatcgc tggcagcatg aagcagttat tgcccttgcc acccccgaca atgtgaacgc
    13321 ttttgaactg acaccgcagg aagcggagga gtggtatcgc gggcgcgatg tttacccgca
    13381 agccgcgcca gtggcggatg acgtgttggt tactttccag catcaaccga ttggtttagc
    13441 caaacggatt ggttcgcgat tgaaaaacag ctatccgcgt gaactggtgc gcgatgggaa
    13501 actttttacc ggtaacgcct gacagcgcac aaaaaaagcg cactttttga ctggcacatt
    13561 cggctgcctc aactaggctg aaaaatggtg cgatcggact ggtcgtacca caaccggcag
    13621 ctaaatggag agcacgaaga tgaaaaccag tgtgcgcata ggcgcttttg aaatcgacga
    13681 cggcgaatta cacggtgaat cgccaggtga tcgaacgtta accattcctt gtaaatctga
    13741 ccccgattta tgtatgcaac tggatgcctg ggatgctgaa accagtatcc ctgccctgct
    13801 aaatggcgaa cactctgtcc tttaccgtac ccgttacgat caacaatctg atgcctggat
    13861 tatgcgtctt gcctgatcca aaaagaaccc gtcggcatgg cgggttattt gtcctggtta
    13921 ttcccccgtt gtaaaatctc tcctaaactt aacggtacgg caccacactt cggggatgaa
    13981 atgttcgcgc tggtactttt tgtttgctac ctggatggcg gttgtgaaga tattgttgtg
    14041 gatgtctaca acacggaaca gcagtgtctt tattctatga gcgatcaacg gatccgccat
    14101 ggcggttgtt ttccgattga ggattttata gatggtttct ggcgaccagc acaggagtat
    14161 ggtgattttt aattattgca attgcacaag agtcagttcg cccccaaaga cagcaccggt
    14221 atcaatataa tgcaggttgc caatatccac gcgatgtcgc aacggtgtat gaccaaacca
    14281 gaaatgatca gcacctgtaa ttccctgccc tttttggcgt tcacctaatc gcgagcggct
    14341 ccacaagacc tgatgcaaat caacgtcctt ttgccattca taaacatcat ctggataatc
    14401 ggcatgagca ataacatgtt tgccggtacg actgtgtact tcaagaataa agggcaaatg
    14461 ctgacatttt tccagcgccg ttttcgcttg tttctgttga ttatctgcca gcgcaataaa
    14521 ccagtcgccg ccattcatca accacaaaga catctgctgg gatgccagcg catccatcgc
    14581 catctgttca tgattgcctc ttaccgcaca aacccaatgt tgttccagta actgcagaca
    14641 acgtaaactt tgcggcccac gatcgataac gtctcctact gagataagta aatctcgcca
    14701 cggatcaaaa cgacaatgcc ataatttgcg gcgcaactgc tcaagacaac cgtgtatatc
    14761 gccagaaagc cagatatgtc gccattgatg acccgcaatt ctctgataaa cgggcgcagg
    14821 ctgtttcatc aatattttcc tcccgcgcta aagatcacat aatcttaaca agaatgttaa
    14881 aaaacgctgg actcagacag tagagtgtgt gttatggttg actataaagt cagcgaagga
    14941 aatgcttctg gcttttaaca gataaaaaga gaccgaacac gattcctgta ttcggtccag
    15001 ggaaatggct cttgggagag agccgtgcgc taaaagttgg cattaatgca ggcttagttg
    15061 ccttgccctt taagaataga tgacgacgcc aggttttcca gtttgcgtgc aaaatggtca
    15121 ataaaaagcg tggtggtcat cagctgaaat gttaaaaacc gcccgttctg gtgaaagaac
    15181 tgaggcggtt tttttattgg aaatcaaaag gctattttag gtaattaaca gagtttttca
    15241 gctcgttcta taaacggtgc cagactcatt ttttcgccgg gattgttagg atcatcaatc
    15301 tgaatcaccg aaatgggttg ggctttagtc ttcccactgg caacttcctt ttgtgcgata
    15361 tcgtttaaag gatactgcac gagggtactt gggttaatga catacaaagc attacccggt
    15421 ctgcaagtca gcatcacctc ttcgcgatta aacgcccatt tgtctttacc cacttcaaaa
    15481 cgactgacgg taatcacctg cggtgcagcc agcgccgctg cagaactggt gagtaacaga
    15541 aacgtcagaa tacttttttt catcattttc gtaatccatc aaaaaggttc gagagtcgca
    15601 agcaggctga tgatcaccag cactaccgca cctatcgccc attcgagttt agtcatccac
    15661 tcaaaccagg gtgccgcacg atcttcatct tgcctcatgc gcggtacaag aacataacga
    15721 ttagccagcg caatgaccac cataatcatc accaggatgg ctttgagcaa taagagttgg
    15781 ccccagtaag tcgtaagcgt ggggggaaat ccggtaatca acaatgcatt aagcacgccg
    15841 cttgccagta cgccgatcac cgcaaaatgc ccgcaccagg aaaaacgcat cagcgcctga
    15901 atggcctggt gtcgccaacg gcctttgatg agttgcatac accagagcac cggcagtaat
    15961 cccccaaacc acgccgccgc acaaatcagg tgaatcgcgt gattagtctg atggattttc
    16021 gccgttaccc cttcattcag tgtcgcatgc ccgattcccg ccagcagaat aaattgcgcg
    16081 gtagtgagca taaacagcaa acgtggcata ttccgcggtt gcataagggc gacgatcaac
    16141 gttacgaggg cgagaacaat ttgccataac cagacgccac caaactgcgt ttgcaaaaca
    16201 gcctgccaaa tgtttggcga aaatacatct gcccatcccg ttcccatcaa cccgccctga
    16261 acggcgagca ttgcagtggc actgatcaaa ctccagacgg cagcgtgctg ttgtaaacgc
    16321 agaaaacgct tcgccagcaa gcgacgaatc gttaaggggg ccagccaggc accgtacatt
    16381 gcgaagccaa agaccagcat cagcgaggtg aaatggataa atcgcagcgc gatccaggta
    16441 aacgccagca tgatttattt cacgctaaag gtgtagtgtc ctttcgtttt atgcccgtcc
    16501 accgaaacaa catgccagtc aacgatgtag gttccgggtt tcagcgaatc ggccagtgga
    16561 acaatcagtt gcttttggtc ctgctcattt cgcttcgccg gtaatgtttt aatattttcg
    16621 ttttttggcc ccgtgatttt tgcaccactg aatcctgttt caacaccttc cgagaagttt
    16681 aaagtgattg cctgcggtgc agctgtcact tgcgcgtttg ccgcaggata ctgatgcgtt
    16741 aaatgcgcat gtgcccagac ggaaggtgtc accaacgaag tggtcaggat tgccagcgcg
    16801 tagcgaaggg agcgtgcagt tgaagtcata ttatctattc ctttttgtaa taactttttt
    16861 acagagcata accttgtcta atgtctgagt cgaggatcat caattccggc ttgccatcct
    16921 ggctcactct tagtaacttt tgcccgcgaa tgatgaggag attaagaatg ctgaagaatc
    16981 tggctaaact ggatcaaaca gaaatggata aagtgaatgt cgatttggcg gcggccgggg
    17041 tggcatttaa agaacgctac aatatgccgg tgatcgctga agcggttgaa cgtgaacagc
    17101 ctgaacattt gcgcagctgg tttcgcgagc ggcttattgc ccaccgtttg gcttcggtca
    17161 atctgtcacg tttaccttac gagcccaaac ttaaataaaa cttatacaga gttacacttt
    17221 cttacataac gcctgctaaa ttatgagtat tttctaaacc gcacccataa tttgcagtca
    17281 ttttgaaaag gaagtcatta tgtcgttctg gaaagttgca gcagcgcaat atgagcctcg
    17341 taaaacctca ctcaccgaac aggtagctca tcatctggag tttgtgaggg ccgccgccag
    17401 gcaacaatgt cagctgttag tttttccatc gctttcttta ctggggtgtg attattcgcg
    17461 acgtgccctt cctgccccac ccgatctctc actgttagac ccgctttgtt atgccgcaac
    17521 gacctggcga atgaccatca ttgccggcct tcccgttgaa tataacgatc gctttattcg
    17581 tggaattgcg gtgttcgcac cgtggcgaaa aacgcctggg atctaccatc agagtcatgg
    17641 tgcatgtctg ggtcgtcgtt ccagaaccat cacggtggtc gatgaacaac cggaaggtat
    17701 ggatatggac ccgacctgtt cactgtttac tacagggcaa tgtctgggag aacccgacct
    17761 gctggcgtcg gcccgtcgct tacaattttt ttcacatcag tactcgattg ccgtgctgat
    17821 ggcaaatgcc cgtggtaaca gtgcactatg ggatgaatat ggtcgtctca tcgttcgcgc
    17881 cgatcgtggt tcattattgt tagtcggtca gcgttcatca cagggttggc aaggcgatat
    17941 cattccatta cgctaggctt tttcggcctg gagcatgcca tgttgcgcat tatcgataca
    18001 gaaacctgcg gtttgcaggg agggatcgtt gagattgcct ctgttgatgt cattgacgga
    18061 aaaatcgtca accccatgag ccacctggtg cgccccgatc gtcctattag tccacaagcg
    18121 atggcgattc atcgcatcac cgaagccatg gtcgccgata aaccgtggat tgaagatgtg
    18181 atcccacact attacggtag tgaatggtat gtcgcgcata acgccagctt tgaccgccgc
    18241 gtactgcctg agatgcccgg tgagtggatt tgcactatga aactggcccg tcgtttgtgg
    18301 cctgggatca agtacagcaa tatggcgtta tataaaacac gcaagctcaa tgtacagacg
    18361 ccgccgggcc tgcatcatca ccgcgcgttg tatgactgtt atatcaccgc cgcgttgctt
    18421 atcgatatta tgaacacctc cggctggacg gcagaacaga tggccgatat caccggacgt
    18481 ccgtcgttga tgacgacctt cacctttggc aaataccgtg gcaaagcggt ttccgacgtt
    18541 gctgaacgcg atccgggcta tctgcgctgg ttatttaata acctggacag catgagcccg
    18601 gagctgcgtt taacactgaa acattatctg gaaaatactt agtccgcagg cgtagcgggt
    18661 aatgttccct gcgccagcgc gaccagaaaa gcatattcca tcgctacgcc ttcgtacgat
    18721 ttaaagcgac cagatttacc gccatggcct gagtccatgt cggtacagag caataaaaga
    18781 tggtcatcgg ttttcagctc gcgcaattta gcgacccatt ttgccggttc ccaatattgc
    18841 acctgagaat cgtgcaaacc ggtcgttacc agtaaatgcg gataagcctg tgcggtgacg
    18901 ttgtcatatg ggctgtagct tttcatgtac tcgtaatatt gcggatcctg cggattcccc
    18961 cactcttcaa actcaccagt ggtaagagga attgattcat caagcatcgt tgttacaaca
    19021 tcaacaaacg gtacctgggc gataacgccg tggaataatt ccgggcgttg attaattgca
    19081 acgcccatca acatgccccc cgcactcccg cccatcgcat aacaaagcga aggagagcca
    19141 tagcccagtt ttaacaatgc atcgcaggca tcaagataat cattaaacgt atttttcttt
    19201 ttcagaaatt ttccgtcttc gtaccattgt tgccccagct caccaccgcc gcgaacatgg
    19261 acaatggcgt agacaaagcc acgatctaac aaactcaagc ggctaaaact gaaatcggca
    19321 tcaatacttg cgccgtaaga accatagcca tacaccagca acgggttgtg tcctttgcga
    19381 aaatgtttgc gatggtagac caacgaaacc ggaacttcga cgccatcacg ggcgactatc
    19441 cacaggtgtt cactgcggta attcgccgca tcaaaaccag gaacttccgt ttgttttaat
    19501 acacgacgct caccggtatc catatccagt tcaaacaaag tgtctggtgt ggtcatggaa
    19561 gaataaccat aacgcaatcg cgcggtttca gattctggat tgtaggcaat ccaggtcaca
    19621 taggccggat catcaaaggc aataccaatg acttcccggg tcttgcggtt aatttggcgc
    19681 aaactggtta acccgcgctg acgctcttca accaccagcc agtcggtaaa cagcgtaaac
    19741 ccttccagca tgatgttttc gcgtggcgga attaactctt cccactgttg ctcatcacgc
    19801 atacgggtac ggtataagcc aaagtttttg ccgtggcggt tggaacgcag ataaaaacga
    19861 tgctggtagt gatcaaggct gtattcgtga tctttgcggc gcggcagaaa aacaaacggc
    19921 tcggcatcgg ccatttccgc gtccagcagg cgaacttcac tggtggtggc gctggccaaa
    19981 tgaatgacta catagtgctt cgaggtcgtt ttatgcaggc tgacgtaata ggtatcgtct
    20041 ttttcttcgt agatcagttt atcttgcgat gctggcgtac cgatggcgtg acgccagacc
    20101 tgataaggca gcagcgtcac cggatgcttg cgaacatagt agaaagtcca ggagtcattt
    20161 gcccagacaa agctgggttc aacgttatcc agcagttccg ggtaccagtt accagtttcc
    20221 agattacgaa aacgaatgcc gtactggcgt cgggaaagaa aatcttctgc cagcgccata
    20281 atggtgttat cgggcgtaat cgccattccg cccatcgaat aaaactcact atgagctgcg
    20341 cgcttattgg catcgagcaa tgtttcccac tcatcccact cttcactgaa tgccgattga
    20401 cgctggtaga tagcatattc acagcctggt tcataaatat gccgatagcg gtagccattt
    20461 ttgatgtagg gcgcagaaac ttctcgttgc ggaatgcggt cgatgatttc ctttaagatg
    20521 cgatcctgca aggcttgttg tgaggccatc acccgatgac cgtaactatt ttcttgttgc
    20581 aggtagtcca ggacttctgg ctgagaacgc gtatcgtccc gcagccagta gtaattatcg
    20641 atgcgcgtat cgccatgaag cgtcatggcg tggggaatgc gggcggcttt tggtagcatg
    20701 ttattgttct ttctggttga aacatcttat aagggtggca aaactcaccg gggatgcaag
    20761 cgaaacaggg gagtcattgc ttagatgatg acaggtaatg gcgcggatat cgaatgttat
    20821 gcagacagag aaaatcagcc tgttcaaatg gctgtgcgat tctggatagc ccgaaatagt
    20881 caacttcagg ctatccagag agcggaatta ttccgccaaa gtgcgttttt gctgttcgag
    20941 atcgcgttca atgccgtcac gaacatcctg ggggattttc agcgcgtcac ccagtgcatt
    21001 caggtaactg cgttccataa aatggtcaat atcaatagcc gcgcaactca ggaaatagat
    21061 ttccagcgcc tcttcttcat tgcggacccc ggtagccagg cgttgtggat ccagcggttg
    21121 ttcgattgcc tgctcaatga gtacacgccc ctgctcttcc acgccggctt cacgcaattg
    21181 ctggtcgatt gccgcacgtt ctttggcatc aatatgacca tcacttttag cggcaaagac
    21241 cagcgcaagg atcaaacgtt ctgtacgctc atccagcggc gtactttgcg cgccaaactg
    21301 cggttcgtcc tgatgcgccg cgcgaatttt atctttgtat ttattccaca gcaccgtacc
    21361 cgctaccgct ccgccgccaa ccagtaacgc gttggtgcca tatttcgtaa gaagtttacg
    21421 tgctgattta tttgcgacca gcagcccagc cagtccgcct aatgcgcctg gcactaacag
    21481 tttgcccaat ccctgatccg cagacgagga ggtagaagaa ctgctttgcc caagcaggga
    21541 ttgcagttga tttaaccagt tagccatatt tgctcctcaa taaccatttt tagtgcctgt
    21601 agcatagcgg atgaggatgt cagaaaatgt atatcgagac aaaagatgcg caaataagcg
    21661 gggcggtagg ccgttattca aagaaattcg cgccaggtaa ggtgtttact gtatatcagg
    21721 tgataagggt attttaggtg aaagtagttt gacgttttga gtggcactgt atgatgctga
    21781 ttgtccgatg ctacgctgtt gcgtcttctc aggcctacgg ttgtggtgtg ttgtgggcgg
    21841 acaaggcact cgcgccgcat ccgccaacgg tcaatcgaat tatcagaagc cattaacgcc
    21901 gctgatattc cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac
    21961 ttttaccacg tacggttagc ggtactgacc atttatcatc tttaccctga atgtcctgca
    22021 aactgaccca agccacggga tcggcctgac cgacaatttt ttgatcatct gcccaacgcg
    22081 ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc aattccggca gcatccagac
    22141 cttcacactt tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg
    22201 cgcaggcaga aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca
    22261 tagcacaatg attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg
    22321 ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt
    22381 tgccggtttt cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaagag
    22441 cttcacgttg ctcgtcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat
    22501 ccgccgtatc aagcattttg tcataagcat cggcttcctt tttgctggta aacgacattt
    22561 tctcttcgcc ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat
    22621 aataactgtg attttataca gtatatttct tttcggttga gaaatcaaca ccagcaataa
    22681 agacacacgc aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta
    22741 tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg
    22801 gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg
    22861 tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata
    22921 tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc
    22981 cggagatcga agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg
    23041 ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg
    23101 cggcagaaga gctgcagctg tccacttcca cttatcgttt tgccgatagc gaaagccttt
    23161 tccgcgaggc ggttgccgcc attggctatc cctgcattgt aaaaccggtg atgagctctt
    23221 ccggcaaggg gcagacgttt attcgctctg cagagcaact tgctcaggca tgggaatacg
    23281 ctcagcaagg cggtcgcgcc ggagcgggcc gcgtaattgt tgaaggcgtc gttaagtttg
    23341 acttcgaaat taccctgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccag
    23401 taggtcatcg ccaggaagat ggcgactacc gtgaatcctg gcaaccacag caaatgagcc
    23461 cgcttgccct tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt
    23521 atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaggtct
    23581 cccctcgtcc acatgatacc gggatggtga cgttaatttc tcaagatctc tcagagtttg
    23641 ccctgcatgt acgtgccttc ctcggacttc ctgttggcgg gatccgtcag tatggtcctg
    23701 cagcttctgc cgttattctg ccacaactga ccagtcagaa tgtcacgttt gataatgtgc
    23761 agaatgccgt aggcgcagat ttgcagattc gtttatttgg taagccggaa attgatggca
    23821 gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg
    23881 cgaagcacgc cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa
    23941 aaatgcccga tcgtagatcg ggcgttttga cttttacagc ttagcgcctt ctacagcttc
    24001 acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag
    24061 ccaggaacca ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg
    24121 agaaatacca cccgtcgggc agaaacggac ctgggagaac ggacccgcga tcgcctgcag
    24181 ggctttcacg ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc
    24241 catacccagc atcagttcgg aaacagtgct gatccccgga attagaggaa tagtcccttc
    24301 ggtagcagct ttcagcagcg gctcggtcag acccgggcta attgcgaact gtgcacccgc
    24361 ttcagtgact tctgccagct gctgtggatt cagtaccgta ccggcaccca caatcgcttc
    24421 aggcacttct ttggcgatag cacggatagc gtcaactgca cactcggtac gcagagtcac
    24481 ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc atcggcactg cgtgttccag
    24541 tttttttacc acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt
    24601 tttccagttt ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt
    24661 gatacaggtt gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc
    24721 acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag
    24781 ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc
    24841 cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc
    24901 tgacggcact ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa
    24961 acaccggtcc aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt
    25021 cgcttttggc ccctgatgac ggacaacaac gacacaatcg cggtccagca aacccgcttc
    25081 aaaggccggc ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg
    25141 gttctcaacc ggcacggcag aggttttcat aaccgcacgg cccaggttac cgcttaacac
    25201 ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag
    25261 tgatttttcc gccccttccc gccagtccag ttcaccatta ttcagccatg gttcaagggt
    25321 ataacgagac agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt
    25381 gagcagttca cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc
    25441 cggaccgttc gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc
    25501 atcccagtta atctgaatac cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt
    25561 agtggaacca ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat
    25621 catcttaccg atcggcatcc attcattacc attaccggtc atgcgtgtaa cctgacgcgc
    25681 agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg
    25741 caactgcatc cccataaact ccaccaccat ctggttggtg ttggcagtac cgtagaaagt
    25801 acatgttccc ggcgcgtgat aagacgcggc ttctgactcc agtagtgcca tgcggtccac
    25861 tttaccttcg gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc
    25921 catcggtcca gacggcacaa acaccgcagg caaatgacca aacgacaggg ctgccatcgt
    25981 cagacccggg acaatcttgt cgcacacacc gaggaacaga gcaccatcaa acatgttatg
    26041 ggacagcccg accgccgcag acatcgctat cacttcgcgg cttagcagcg acaattccat
    26101 tccatcctgc ccctgggtga caccatcaca catcgccgga acaccgcccg caacctgacc
    26161 aaccgcattc gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg
    26221 gtgagcggag agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct
    26281 tttcaaagag gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca
    26341 tgccaactgc gaacgatgaa cagtcgacgt tttcgcttgt tctatccggg cgagataagc
    26401 agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt acgcgtaaca attgtggatt
    26461 cataaaggct cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcattcag
    26521 aggatttatg actgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt
    26581 gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct
    26641 acaatctgtg caagatcatg ttaccggtaa aataaccata aaggataagc gcagatatta
    26701 ctcaaactca ttccaggaac gaccatcacg ggtaatcatc gccaccgagg caacgggtcc
    26761 ccaggttccg gcctgatacg gtttcggcgc atctttgtcc atcgcccacg cctcagtaat
    26821 ggagtctacc catttccagg cttcttccac ttcgtcgcga cgaacaaaca gtgcctgaat
    26881 accacgcatg gtttccagca gcaaacgttc ataggcatcc gccagatgcg tctgattaaa
    26941 ggtttctgaa tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc
    27001 aggaacttta ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag
    27061 tttattctgc ggcagatcct gccacgactc tttaaacaga ttcagttcag gtgttttgaa
    27121 atagaccacg acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg
    27181 cacaccggcc cagcgccagt tatcaatgtc gacgcggatc gccacgaagg tttctgtatt
    27241 gctgctcttg ttcgcgccct cttcttccag atatcccggc acttttttgc cctgggcgaa
    27301 gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg
    27361 cagagacttc agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagacgg
    27421 cggagacatc gcaatcatgc agagaatttg cagcaggtgg ttttggatca tatcgcgcat
    27481 ctgaccggct ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt
    27541 aatctcaaca tgatcaatgg tgcgattgtc ccagttattc acaaacaggg agttagcaaa
    27601 acgcagcgcc aacaggttca gcaccgtttc tttaccaaga tagtggtcga tacggtaaac
    27661 ctggcactcc tcgaagtatt cgccaacctg atcattgatt tcctgcgagg tcgccagcga
    27721 cgtccccagc ggtttctcca taactacgcg tgccggttta gcattcagtt ttgcctcacc
    27781 aagccctttg caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat
    27841 acgatttttt tgatccagca tcgcgccgag acggctgaat gcagcagtgt cattgacatc
    27901 gagattacaa aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatagtttc
    27961 tttcatgaaa gtttcgagcg cctcacggac aactttggta taagccgctt tatcccagtc
    28021 agcacgccct acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg
    28081 atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag
    28141 gtcacaggcc tgggctgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg
    28201 gtacttaagc cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc
    28261 ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg gtaaaagcgc gcaaagtgcg
    28321 gcaaaactga taaaaaaatc atcgttttcc attgggtaaa aatctgacac tgatcatgtt
    28381 atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt aacacagcac acaggcgtaa
    28441 tatctgacaa aactgcattt cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc
    28501 tctttacatc atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc
    28561 agagcgcaag gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat
    28621 tgctgctctg gcgctggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag
    28681 catggacacg cgcggttttc ctgattttaa acttcatctg gcacaaagtc tggcgaatgg
    28741 cactccctat gttaatcgca atgtcaatga agatgacagc gttgaatcat acacagggaa
    28801 aatatttgag tccgcaatgg caacgcttga tcatgtccgt cattcactgg ataaatctgc
    28861 catcaaccgc gccgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg
    28921 ctcttcagcc gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt
    28981 ggtgtactcc gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga
    29041 cgtggtggtg ctgatttctc acactggaag aacaaaaaat ctggtcgagc tggcgcagct
    29101 ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct gcgggtaccc cgctcgcccg
    29161 ggaagcaacg ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat
    29221 ggtttctcga cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg
    29281 acgcggtgca aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg
    29341 ttttgataag cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta
    29401 atcttatacg acatccgaat gagattaatt tatcgccatc gcggcgttat ttcattcgga
    29461 tttcatgttc aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc
    29521 ttcgcagaac aaaaatcgtt accacgttag gcccagcaac agatcgcgat aataatcttg
    29581 aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa cttttctcac ggctcgcctg
    29641 aagatcacaa aatgcgcgcg gataaagttc gtgagattgc cgcaagactg gggcgtcatg
    29701 tggctattct gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca
    29761 aagttttcct caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag
    29821 gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg
    29881 acatcctgct gctggacgat ggtcgcgtcc agttaaaagt actggaagtt cagggcatga
    29941 aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac
    30001 ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg
    30061 cggcgttgat tggcgtagat tacctggctg tctccttccc acgctgtggc gaagatctga
    30121 actatgcccg tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg
    30181 aacgtgcgga agccgtttgc agccaggatg caatggatga catcatcctc gcctctgacg
    30241 tggtaatggt tgcacgtggc gacctcggtg tggaaattgg cgacccggaa ctggtcggca
    30301 ttcagaaagc gttgatccgc cgtgcacgtc agctaaaccg agcggtaatc acggcgaccc
    30361 agatgatgga gtcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag
    30421 caaacgccgt tctggatggt actgacgctg tgatgctgtc tgcagaaact gccgctgggc
    30481 agtatccgtc agaaaccgtt gcagccatgg cacgcgtttg cctgggtgcg gaaaaaatcc
    30541 cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcta
    30601 ttgccatgtc ggcaatgtac gcggctaacc acctgaaggg cgttacggcg atcattacca
    30661 tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctgccaattt
    30721 tcgccatgtc gcgccatgaa cgtacgctga acctgactgc tctctatcgt ggcgttacgc
    30781 cggtgcactt tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc
    30841 tgcgcgataa aggttacttg atgtctggtg acctagtgat tgtcacccag ggcgacgtga
    30901 tgagtaccgt gggttctact aataccacgc gtattttaac ggtagagtaa gtacgttgcc
    30961 ggatgcggcg aaaacgccac atccggccta cagttcaatg atagttcaac agatttcgaa
    31021 tattctgaag caaacttgaa cttatcatca ggcgaaggcc tctcctcgcg agaggctttt
    31081 tttatttgat gggataaaga tctttgcgct tatacggctg gatttcgccc ggtttgcgag
    31141 ttttcagcaa ttttagtatc caggtgtatt gttctggtcg cggaccaaca aaaatctcga
    31201 cttcttcatt catccgccgc gcaatcgtat gatcatccgc ctctaacaga tcatccatcg
    31261 gtgggcgcac ctgaatcgtc agacggtgcg tcttgccatc ataaatcgga aacagcggaa
    31321 caacgcgcgc acggcacact ttcatcaaac gaccaatcgc gggcaacgtc gctttatagg
    31381 tggcaaagaa atcaacaaat tcgctgtgct cagggccatg atcctgatcg ggtaaataat
    31441 atccccagta gccctgacgt accgactgga tgaatggttt aataccatca tttctcgcat
    31501 gcagacgacc gccaaaacga cgacgcaccg tgttccagac ataatcaaaa accgggttgc
    31561 cctgattatg gaacatcgct gccattttct gcccttgcga ggccatcagc atggcaggaa
    31621 tatcgacggc ccaaccgtgc ggcaccagaa agataacttt ctcgttatta cgccgcatct
    31681 cttcgatgat ctccagccct tgccagtcaa cgcgcggctg aattttctcc ggcccgcgta
    31741 ttgccaactc agccatcatt gccatcgctt gcggcgcggt ggcaaacatc tcatcaacaa
    31801 tcgcttcgcg ttcagcttca ctacgttctg gaaagcagag cgacagattg attaacgcac
    31861 gacggcgtga gctttttccc agtcgtccgg caaaacgtcc cagccgtgcc agaatgggat
    31921 cacggaactt tggcggcgtt aaagcgatac ccgccatcgc tgctacgccc agccatgctc
    31981 cccagtagcg cgggtggcga aaggatttat caaactcagg aatgtattcg ctattatttt
    32041 ttttcgtttc catgcttttc cagtttcgga taaggcaaaa atcaatctgg tgatagtgta
    32101 gcggcgcaac ttgccccgca ccaaataaaa aaagccggta ctgacttcgt accggctgcg
    32161 aatggatgtt aattaatcaa accgtagctg cggcacaatc tctttggcct gcgccaggaa
    32221 ttcgcgacga tcggagccgg tcagcccttc ggtacgcggc agttttgccg tcagcgggtt
    32281 tacggcctgc tggtttatcc atacttcata gtgcagatgc ggcccggttg aacgtccggt
    32341 attaccggaa agcgcgatac ggtcgccacg tttcaccttc tgtcccggtt tcaccagaat
    32401 cttgcgcaag tgcatataac gcgtggtgta gctgcgacca tgacgaatag ccacataata
    32461 acctgctgca ccactgcgtt tggcaaccac cacttcaccg tcacccactg aaagcactgg
    32521 cgtaccttgt ggcatggcga aatcaacacc tctgtgtggt gcaacgcgac cggtcaccgg
    32581 attagtacga cgcgggttaa agttagacga gatacggaac tgtttcgccg tcgggaatcg
    32641 caagaatcct ttcgccagac cagtaccgtt acgatcgtag aatttgccat cttcagcgcg
    32701 gatagcgtaa taatctttac cttctgaacg caaacgtacg cccagcagct ggctttgctc
    32761 acgtttacca tcaagcattt ctcgagacat taataccgca aattcatcgc cttttttcag
    32821 tttgcggaaa tccatttgcc actgcatggc tttaatcact gcgctcactt cggcgctggt
    32881 taaaccggcg tttctggcgc tggcaacaaa gctccccccg acggtacctt tcagcagatt
    32941 gttaacccac tctccttgct gcatttcgct ggtcatttta aaaccgttag cggcagtacg
    33001 gtcataggtt cgggtttcac gacgagacac ttcccaggtg aggcgctgca gttcgccgtc
    33061 cgcggttaat gtccaggaga gttgttgacc gattttcagg ttacgcaatt ctttgtcggc
    33121 cgcagccagt tgggtgatat cacccatatc aataccatac tgattgagaa tgctgcttag
    33181 cgtatcgcca gtggaaacaa catattcatg cacgcccgct tcaccggcga ttttgtcatc
    33241 cagttcgtcc tgaggaatgg cttcatcttc ttgtgcagct tgatcaatcg gctcactggc
    33301 ttcaggtaag agcgaacgaa tttcgttctg ttccagctca atggttttga caattggcgt
    33361 ggcatcgcgg tgataaacat agggccgcca gacagcgacg gccagagtaa gaacggtgag
    33421 cgaccccaac ataacgcggt gtggtcgcgg taaattatta aacgccaggg cgacagagcg
    33481 ggctatctgt tgcacgtaat cacttcctca ttaatctcct ttcaggcagc tcgcatactg
    33541 gttggctaat tgactcagga attctgaata gcttgtttta cccagtttga tattcgtccc
    33601 caggggatcc aacgttccca tacgaacgga tgtccctcgt gcgacgctct caacgaccgc
    33661 tggcctgaac tgtggctcag caaaaacgca ggttgctttt tgctcaacca actgtgttct
    33721 tatttcatgt aaacgctgcg cgccaggttg aatctcaggg ttaacggtaa aatgaccaag
    33781 cggtgtcagt ccaaactgtt tttcgaaata gccgtaagca tcgtgaaaaa cgaaataacc
    33841 tttccccttg agcggcgcga gctcgttacc aacctgcgtt tcggttgagg ctaattgtgc
    33901 ctcaaaatcc ttcaggttgg cgtcaagttt ggctcgactt tgcggcataa gttccactaa
    33961 ttttccatgg attgcaaccg ctgtagcccg cgctatctct ggggaaagcc aaagatgcat
    34021 gttgaaatcg ccgtgatggt gatcttcgtc acttttttcc gcgtggtcgt gatcatcatc
    34081 atcgccgtga atacttttca tcagtagcgg tttcacatct tcaagctgcg caatcgttac
    34141 ctgcttcgct tctggtaatt tgcttaccgg tttttgcata aacgcttcca tctccgggcc
    34201 aacccaaacg actaagtccg cgttctgtaa gcgttttaca tccgatggac gcagtgaata
    34261 atcatgttct gaagcgccgt caggcagtaa aacctgcgtt tctgttacac catcagcaat
    34321 ggcagaagcg atgaacccaa cgggtttaag cgaagcgaca acggcggcat ctgcggcctg
    34381 tgttgcacct ccccagagag cggcggataa tgctgcgaaa agaagcgttt ttttatgtaa
    34441 cataatgcga ccaatcatcg taatgaatat gagaagtgtg atattataac atttcatgac
    34501 tactgcaaga ctaaaattaa catgacaagt ctggtttccc tggaaaatgt ctcggtttct
    34561 tttggccaac gccgcgtcct ctctgatgtg tcgctggaac ttaaacctgg aaaaattttg
    34621 actttacttg ggccaaatgg cgcaggtaag tcgacactgg tacgggtagt gctcgggctg
    34681 gtaacacccg atgaaggggt tatcaagcgc aacggaaaac tgcgcatcgg ctatgtaccg
    34741 cagaagctgt atctcgacac cacgttgcca ctgaccgtaa accgtttttt acgcttacgc
    34801 cctggtacac ataaagaaga tattttgcct gcactgaaac gtgtccaggc cgggcacctg
    34861 attaacgcac cgatgcaaaa gctctctggt ggcgaaacgc agcgtgtact attagcgcga
    34921 gcattgttaa atcgcccgca attattagtg ctggatgaac ccactcaagg cgtggatgta
    34981 aatggccagg tggcgttata tgaccttatt gaccaactgc gtcgtgaact ggattgtggc
    35041 gttttaatgg tttctcacga tctgcatctg gtaatggcaa aaaccgatga agtgctgtgt
    35101 ctgaatcacc acatttgttg ttccggcaca ccggaagttg tttccctgca tccggagttt
    35161 atttctatgt ttggtcctcg tggtgctgaa caactgggta tctatcgcca tcatcataat
    35221 catcgtcacg atttacaggg acgaattgtt ttgcgtcggg gaaatgatcg ctcatgattg
    35281 aattattatt tcccggttgg ttagccggga tcatgctcgc ctgtgccgcg ggtccgctgg
    35341 gttcgtttgt agtctggcgt cgtatgtctt atttcggtga tacgctggct catgcctcat
    35401 tacttggcgt cgcgtttggt ttgttgctgg acgtgaatcc attctatgcg gtaattgccg
    35461 ttacgctgct cctggcgggc ggtctggtat ggctggagaa gcgtccacag ctggcgattg
    35521 acacgttatt agggattatg gcgcacagtg ccctgtcgct gggcctggtg gtcgttagtc
    35581 tgatgtctaa cattcgtgtt gatttgatgg cttacctgtt cggtgatttg ctggcagtga
    35641 cgccagaaga tctcatcgct attgcgattg gcgtggtcat cgtggtggct attttgttct
    35701 ggcaatggcg caatttgctg tcgatgacga ttagcccgga tctggcgttt gttgatggtg
    35761 tgaaattaca gcgcgtgaaa ttgttgttga tgctggtgac ggcattgacg attggtgtag
    35821 cgatgaaatt tgttggcgcg ttgattatta cttcactgct gattattcct gcagctactg
    35881 cgcgtcgctt tgcccgtacg ccggaacaga tggctggtgt cgctgttttg gtggggatgg
    35941 tggcagtaac tggcggttta accttttccg cgttttacga tacgccggcg ggtccgtcgg
    36001 tggtgctatg tgcggcactg ttatttattc tcagtatgat gaaaaagcag gccagctaat
    36061 ctatcgctga acacatttgt cggatgcggc gcgagcgcct tatccgacct acggttcgct
    36121 atctctggct ggcctgataa gacgcgaaca gtgtcgcatc agacacactg ccagtgtcgg
    36181 atgcggcgcg agcgaccaat ccgacttacg gcatttctgg cggcgttatg ccaaagtgat
    36241 tccacgcccg cgtcgtcgcc atacgcccac gcggtgtacg ctgcaaaaag ccttgctgaa
    36301 tcaaataagg ttccagcaca tcctcaatgg tttcacgttc ttcgccaatg gctgccgcaa
    36361 ggttatccag acctaccggc ccaccaaaga acttatcgat taccgccagc aataacttgc
    36421 ggtccatata atcgaaacct tcagcatcga cattcaacat atccaacgcc tgagcagcga
    36481 tatctgccga gatggtgcca tcgtgcttca cttcggcgaa atcacgcact cgacgcagca
    36541 gacggttggc aatgcgcggc gtaccgcgag ctcgacgagc aacttccagc gcgccgtcat
    36601 cactcatctc aagccccata aagcgtgcgc tgcgactgac gatatattgc agatccggca
    36661 cctgataaaa ctccagacgt tgcacaatac caaaacggtc gcgcaacggt gatgtcagcg
    36721 aacctgcgcg cgtggttgca ccaatcaggg taaacggcgg caaatcaatt ttaatggagc
    36781 gtgccgccgg accttcacca atcatgatat ccagttggta gtcttccatt gccgggtaca
    36841 gcacttcttc aacaactggc gataaacggt ggatctcatc aataaacagc acgtcatgcg
    36901 gttcaaggtt agtgagcatc gcagccaaat cgcccgcctt ttccagcacc ggaccagaag
    36961 tcgtgcgtaa attaacgccc atttcattgg cgacaatgtt ggcaagcgta gttttaccca
    37021 accccggagg accaaaaatc aacagatgat cgagggcatc gccgcgcagt ttcgctgctt
    37081 tgatgaaaat ctccatctgt gaacgaacct gcggctgacc aacatactct tccagtaatt
    37141 tagggcgaat ggcgcgatct gctacatctt ccggcaaagt ggtaccggca gaaatcagac
    37201 ggtctgcttc aatcatcctt tacctcataa cgcggcgcgt agggcttcgc gaattaatgt
    37261 ttcactgctg gcgtcagggc gagcgatttt gctcaccatg cggcttgctt cttgtggttt
    37321 atagcccagc gccaccagcg cggcaaccgc ttcctgttca gcatcgtcgg tcgtcgggct
    37381 ggcaggtgac gtgagtacca ggtcggcggc tggcgtaaag agatcgccat gcaaaccttt
    37441 aaatcggtct ttcatttcaa caatcaagcg ttcggcggtt tttttgccaa tacccggcag
    37501 tttcaccagt gcccccactt cttcacgctc aacggcatta acgaactgct gcgctgacat
    37561 tccggagagg atcgccagcg ccaacttcgg gccgacgccg ttggttttga tcaactcttt
    37621 gaacaatgtg cgctcttgtt tattgttaaa accgtacagc agttgcgcgt cttcacgcac
    37681 cacaaagtgg gtgaaaacga tcgcttcctg acccgcttca gggagttcat aaaaacaggt
    37741 catcggcata tgcacttcat agcctacgcc gcccacttca attaacacca gcgggggttg
    37801 tttttcaatg atgatgcctc tgagtctgcc tatcacatga cgctcctgcg taatgaatca
    37861 aagataatgc tgtatgataa aaaaatgctg gatagatatc cagcgaagga tgaagaaaac
    37921 ttgcgaggcg tcccaacgat ctgaaaaatg gcgcagtata atttattcta caggttatat
    37981 tggaagcaaa tatttgaata ttacatattc agtgaagaaa tgcgtaataa aaatatacat
    38041 tgcgactcct gaaataataa attttttatg ctattacgta tattcatatc tatttcaatg
    38101 gaatgacaac gtgaatatta attaccctgc tgaatatgaa attggtgata tcgtctttac
    38161 atgtataggt gctgccttat ttggtcaaat atcagctgca tcaaactgct ggagtaatca
    38221 cgtcgggatc attatcggtc ataacggtga agactttctg gttgcagaaa gccgtgttcc
    38281 cctctcaacc atcactacgc tatcccgttt tattaaacgc tctgctaatc aacgctatgc
    38341 tataaagcga ttagacgccg ggctgacaga acaacagaaa caacgaattg ttgaacaggt
    38401 tccctcccgg ctacgcaaac tttaccacac gggttttaaa tacgaatctt cccgccagtt
    38461 ctgttcaaaa tttgtctttg atatttataa agaggcgcta tgtattccgg tgggtgaaat
    38521 agagacgttt ggacaactgt taaatagcaa tccgaatgca aaactcactt tctggaaatt
    38581 ctggttctta ggttctattc cgtgggagcg taaaaccgtc acgccagcca gtttgtggca
    38641 tcatccgggt ttggtgttga ttcacgcgga gggagttgaa acgcctcagc ctgaactgac
    38701 cgaggcggta taacttaacg cagtcgccct ctcgccaggt tcagccgcga ttcgctcatc
    38761 tgcattgcat tctggctgac gtgacaatgg gtaatcgcga tagctaacgc atcagcggca
    38821 tccgcttgtg gattagcggg cagtttcagc aaggtgcgga ccatatgctg cacctggctt
    38881 ttctcggcgc taccaatacc taccaccgtt tgctttacct gacgtgccgc gtattcaaat
    38941 accggcaatt cctgattcac cgccgccaca atcgccacgc cgcgcgcctg gcccagtttc
    39001 agggctgagt cagcgttctt cgccataaag acttgttcaa tggcgaaata atcaggctgg
    39061 aactgggtga tgatttccgt cacgcccgca tagatgagct tcagacgaga cggtaaatca
    39121 tccactttgg tgcgtatgca tccactaccc aggtaggaca gttgcctgcc tacctggcgg
    39181 atgacgccat agccggtcac gcgcgaaccc ggatcaatgc cgagaataat agccatcacg
    39241 cgtctccgtt ttgctgttta gcaggcctca tcagagagtc gctgcaacct catcagagat
    39301 ttcaccgtta tggtaaactt cctgcacgtc gtcgcaatct tccagcatat cgatcagacg
    39361 catcagtttc ggtgcggttt ctgcatccat atcagctttg gtagacggga tcatggaaac
    39421 ttccgcgctg tctgctttca gacctgccgc ttccagagcg tcgcgcactt tacccatttc
    39481 ttcccatgcg gtgtagacat caatcgcgcc gtcatcatag gtcacaacgt cttcagcacc
    39541 tgcttccaaa gctgcttcca tgatggtgtc ttcatcgcct ttctcgaagg agatcacgcc
    39601 ttttttgctg aacagatagg ctacggaacc atcagtaccg aggttaccgc cacatttgct
    39661 gaatgcatga cgcacttcag caacggtacg gttgcggttg tcagacagac attcaatcat
    39721 gattgccgtg ccgccaggac cgtaaccttc gtagatgatg gtttccatgt ttgcatcatc
    39781 atcaccgccc acaccacgtg caattgcgcg gttcagtgtg tcgcgggtca tgttgttaga
    39841 cagtgcttta tcaattgctg cacgcagacg cgggttagcg tccggatcgc caccgcccag
    39901 cttagccgcg gttaccagct cacgaatgat tttagtgaag attttaccgc gctttgcatc
    39961 ctgcgcagct ttacgatgtc tggtgttggc ccatttacta tgacctgcca taaaaatatc
    40021 tccagatagc cctgcctgtt caggcagcgt taatgacaaa ctgttcaatc gcctgccggt
    40081 tgctccagga cttagtgagc gccgccgcag caggcgcatc aagccacttg taagccagat
    40141 gttcagtgaa aacgatctgc cgctcgtgcg gaagcgcaag acagaaccat gattccgtat
    40201 tacgcgtcac gcccggcgca tagcgatgac gtaaatgtga aaaaatttca aactctaccg
    40261 tgcgctgaca gtcaattaag gtcagttgtt cagcgacaac atcaatggtg acctcttcct
    40321 ttacttcgcg catggcagct tgcggcgcgg tttcaccctc ttccacgctg ccggttaccg
    40381 actgccagaa atcgggatcg tcacgccgct gcaacatcag cacccgtttc gtatcttgtg
    40441 catagatgac cactaagatc gaaacgggac gcttatacac tttatccttc acgctgcctc
    40501 ttcgttgact gccttcgctc atcccattca catagttatc tatgctcatg ggagttcact
    40561 cagttgccgc cttgatgcaa cgcgaatgat ttcgtgtatt tgagtcatat cagttattct
    40621 cagccttctt cacaacctga atgctcagct cagccagtgc agtcgggtta gcaaagctcg
    40681 gtgcttcagt catcagacac gctgccgccg tggttttcgg gaaggcaata acgtcacgga
    40741 tattgtcggt gccggtcagc agcatggtca gacggtcaag accgaatgcc agacctgcgt
    40801 gcggcggagt accgtatttc agagcgtcga gcaggaagcc gaatttctcg cgctgttcct
    40861 cttcgttgat acccagaata ccaaacaccg tctgctgcat atcaccatta tggatacgta
    40921 ctgaaccacc gcccacttcg taaccattga tgaccatatc gtaagcgttc gccaccgcat
    40981 tttccggtgc agctttcagt tctgcagcag tcatgtcttt cggtgaggtg aacggatggt
    41041 gcattgccgt caggccgcct tcaccgtcgt cttcaaacat cgggaagtcg ataacccaca
    41101 gcggtgccca tttgctttcg tcggtcagac caaggtcttt acccactttc aggcgcagtg
    41161 cgcccatcgc gtcggcaaca attttcttgt tgtcggcacc gaagaaaatc atatcgccat
    41221 cttgcgcggc agtacgatcc aggatggctt cgatgatttc tgcattaagg aacttcgcta
    41281 ccgggctgtt gataccttcc agacctttcg cgcgttcgtt aactttgatg taagccagac
    41341 ctttcgcgcc gtagatttta acgaagttac cgtattcgtc gatctgctta cgggtcagcg
    41401 atgcgccgcc cggaacgcgc agagcggcaa cgcgaccttt cggatcgttc gccggacctg
    41461 caaatactgc aaactcaaca gatttcagca gatcagcaac gtcggtcagt tccatcgggt
    41521 tacgcagatc cggtttatca gaaccataac ggcgttctgc ttccgcaaag gtcattaccg
    41581 ggaaatcgcc cagatccacg cccttcactt ccagccacaa atgacgcacc agcgcttcca
    41641 tcacttcacg cacttgcggc gcggtcatga aagaagtttc cacatcgatc tgagtaaatt
    41701 caggctgacg atcagcgcgc aggtcttcgt cacggaagca tttaacgatc tgatagtaac
    41761 ggtcaaaacc ggacatcatc agcagctgtt tgaacaactg tggggattgc ggcagtgcgt
    41821 agaatttacc tttgtgcaca cgagaaggca ccaggtagtc acgcgcgcct tccggcgtgg
    41881 ctttggtcag catcggagtt tcgatgtcga ggaagccgtg gtcatccata aaacggcgca
    41941 ccaggctggt gattttagcg cgggttttca ggcgctgagc catttccgga cgacgcaggt
    42001 cgaggtagcg gtatttcaga cgcgcttctt cggtgttgac gtggttagag tcaagcggca
    42061 gaacatctgc gcggttgatg atagtcagcg aggacgccag cacttcgatt tcgccggtcg
    42121 ccatatcgcg gttaatattt ttttcgtcac gcgcacgtac ggtgcccgtg acctgaatgc
    42181 agaactcatt acgcagttca gaggccagct ttaacgcgtc cgcacgatcc ggatcgaaaa
    42241 atacctgcac gataccttcg cggtcgcgca tatcgatgaa gatcaggcta ccaagatcac
    42301 gacgacggtt gacccaacca cacagagtca cctgctgccc cacgtgggac aaacggagct
    42361 gtccacaata ttctgtacgc atgagatatc ccttaactta gctgccggcg gatgccccct
    42421 gctgcgcagg tgaccaagtc gcagcgttag ctgtatgtca caactgaatg aaaaaaggcg
    42481 gctattatac tggaaattct gccgcaccgt aagagcctgg cccgcgctgg aacgcctcgt
    42541 taccacttta tatcgggcct gaaatcagac tctacgccag tttgctataa aggtgttgcc
    42601 cgaactcata aaaattaaca aaatttgtcg ttccgccatc ggctaatcgc attaaggtgt
    42661 gaggcacgat tttgtttttt caggagtcag catgcttgaa cttaatgcta aaaccactgc
    42721 gttggtggtg atcgatttac aagaaggcat cttacctttt gccggtggtc cacatactgc
    42781 cgatgaggta gttaatcgcg ccgggaagct ggcggcgaaa tttcgcgcca gcggtcagcc
    42841 cgtgtttctg gtgcgagttg gctggtctgc cgattacgcc gaagcattaa aacagccggt
    42901 tgatgccccc tcccccgcta aagtgttgcc cgaaaactgg tggcaacatc cagctgcatt
    42961 aggtgcaact gacagtgata tcgaaatcat caaacgtcag tggggtgcgt tttacggtac
    43021 ggatctggag ttgcaattac gccgccgagg tatcgataca atagtgttat gtgggatctc
    43081 gaccaatatc ggtgttgaat ccaccgcccg caatgcctgg gaacttggct ttaatctggt
    43141 gattgccgaa gacgcctgta gtgccgccag cgccgagcag cacaataaca gcatcaacca
    43201 tatctaccca cgcatcgccc gtgtgcgtag cgtggaagag atccttcacg cgttatgatt
    43261 tacatcggtc taccgcaatg gtcgcatcct aaatgggtgc ggttggggat caccagcctt
    43321 gaagagtatg cccgccactt taactgcgtg gagggcaaca ccacgcttta cgccctgccg
    43381 aaacccgagg ttgtcctgcg ctggcgtgaa cagaccacag atgacttccg cttctgtttt
    43441 aagtttccgg cgaccatttc gcatcaggca gcattacgac attgcgatga tttagtgact
    43501 gaatttttga cccgcatgtc accgttggct ccgcgcattg ggcaatactg gctgcaactg
    43561 cctgccacat tcggcccacg ggagctgcct gcgctttggc attttctcga ttctctccct
    43621 ggcgaattta attatggtgt ggaagtccgc catccacagt ttttcgccaa aggggaagag
    43681 gaacaaacgc ttaatcgcgg tttacatcag cgcggcgtta atcgggtgat tttagacagc
    43741 cgcccggttc atgcagcacg tccacacagt gaagctattc gcgacgctca acgaaaaaaa
    43801 cctaaagttc cggtacatgc tgtactgacg gcgacaaatc cgctgatccg ttttatcggg
    43861 agtgatgata tgacgcaaaa ccgggaatta tttcaggtct ggttacagaa attagcgcag
    43921 tggcatcaga ccactacgcc ttatcttttt ttacatacgc cagacattgc ccaggccccg
    43981 gaactggtac ataccctgtg ggaagactta cgtaaaacgc ttccagagat cggagcagtt
    44041 ccggctattc cacagcaatc ttctcttttc tgaatttgcc acctatcata gacaggtgcc
    44101 atcggccatt ttaaagggag tttgtatggt aagcgcgctg tatgccgttt taagtgcgtt
    44161 gttattaatg aagttctctt ttgatgtcgt tcgcttgcgt ttgcagtacc gcgttgccta
    44221 tggtgacggt ggttttagcg aactgcaaag cgctattcgc attcatggta acgcggtgga
    44281 atatattccc atcgcgattg tgctgatgct gtttatggaa atgaatggcg cagaaacctg
    44341 gatggtgcat atttgcggca tcgttttgct tgctggtcgt ctgatgcatt attacggttt
    44401 tcatcaccgt ctgttccgct ggcgacgttc cggcatgagc gccacctggt gtgcgctgtt
    44461 gctgatggtg ctggcgaatc tttggtatat gccctgggag ttggttttct ccctgcgtta
    44521 gcgcacaata cgccactttc tttttcccgg atttttacgt tatgtctcac cgcgacacgc
    44581 tattttctgc ccctatcgcc agactgggcg actggacctt tgatgaacgg gtagctgaag
    44641 tcttcccgga tatgatccag cgttccgttc ccggctattc caatattatt tccatgattg
    44701 gtatgttagc cgagcgcttc gttcaacctg gtacgcaggt ttacgatctg ggttgttctc
    44761 tgggcgcggc gacgctctcg gtgcgtcgca acattcatca tgataattgc aaaattattg
    44821 ccatcgacaa ctccccggcg atgattgaac gctgccgtcg tcatattgac gcctataaag
    44881 cccctacgcc agtagacgtc attgaaggtg atattcgcga tatcgccatt gaaaacgcat
    44941 cgatggtggt gctgaatttt accctgcaat tcctggaacc ttccgagcgc caggcattac
    45001 tggataaaat ttatcaagga ctgaacccgg gcggcgcgct ggtgctttcg gaaaaattca
    45061 gtttcgaaga tgccaaagtt ggtgaactgt tgttcaacat gcaccacgac tttaaacgtg
    45121 ccaacggtta cagcgaactg gagatcagcc agaagcgcag catgctggaa aacgtgatgc
    45181 tgaccgattc cgtggaaacc cataaagcac gcctgcataa agccggtttt gagcatagcg
    45241 agctgtggtt ccagtgcttt aactttggtt cactggtggc attaaaagca gaggacgctg
    45301 catgatcgac tttggtaact tttattctct gattgccaaa aatcatcttt cacactggct
    45361 cgaaacgctg cccgcgcaga ttgctaactg gcagcgcgag cagcagcacg ggctgtttaa
    45421 gcagtggtcc aacgcggtgg aatttctgcc tgaaattaaa ccgtatcgtc tggatttatt
    45481 gcatagcgta accgccgaaa gcgaagagcc actgagcgcc gggcagatta aacgcattga
    45541 aacgcttatg cgcaacctga tgccatggcg caaagggccg ttctcactgt atggcgtcaa
    45601 catcgatacc gaatggcgtt ccgactggaa atgggatcgc gttctgcccc atctttctga
    45661 tttaaccggg cgcaccattc tggatgtcgg ctgcggcagc ggttatcaca tgtggcgcat
    45721 gattggcgca ggcgcgcacc tcgcggtggg gatcgaccct acgcaactgt ttctgtgcca
    45781 gtttgaagcg gtgcgtaaac tactgggtaa cgatcagcgc gcacatttgt taccgttagg
    45841 tattgaacag cttccggcac tgaaagcctt tgataccgtc ttttcgatgg gcgtgcttta
    45901 ccatcgccgt tcgccgctgg agcatctctg gcagttgaaa gatcaactgg tgaatgaggg
    45961 tgaactggtg ctggaaacgc tggttattga tggcgatgag aacacggtgc tggtaccagg
    46021 cgatcgttac gcgcaaatgc gtaatgtcta tttcatccct tccgcgctgg cgctgaaaaa
    46081 ctggctgaag aagtgtggtt ttgtagatat ccgcattgtg gatgtgtgcg ttaccactac
    46141 tgaagagcag cgacgcactg aatggatggt taccgaatct ctgtccgatt tcctcgaccc
    46201 acatgatccg agtaagacag ttgaaggtta tcctgcgcct aaacgcgcgg tgctgattgc
    46261 gcgcaagccg taaaggtctg gtattactgc cggatgcggc gtgaacgcct tatccggcct
    46321 acaaaccctt gctaattcaa tctattgcag ggactatgta ggcctgataa gcatagcgca
    46381 tcaggcagtt ttacgtttgc ataacctcag cgcccgtttc cgggcgctat tcacttctta
    46441 cgcctgaaca gcaggcttat caaatgccgt taacttcggt gcgttacccg tatatttttc
    46501 gatatacacc agcgcagagt taccggcaca ggcatttgcc agctgcgagc tggggatatc
    46561 cgccgttaac acgttcgcac tgccgttttt acataagcca ttttccagat ctggccatgc
    46621 accttcatgc acgcatacca cgcctttttt gatcccgtca gtcaccaccg cacctgttag
    46681 tatctgacca cgtttgttcc agacacgcac cagatcgcca ttcgcaatac caaatcgagc
    46741 agcatcttcg gtgtgaatag tgatcggttc acgatcggca accgcatatt ttttacgcag
    46801 ttccgcatag ttaagctgac tgtgtaaacg gtgtgccgga tgtgcggtca gaagctgcaa
    46861 ctgcttctcg tcggcggtac ccttccactc atcaggcgca agccaggttg ggtgtgccgg
    46921 gcaatcctta tagccaaatt tttccagcgt tttggagtaa atctcaattt tgccacttgg
    46981 cgtacccagc gcatttttca ccggatcggc gcggaaatca ccataacgaa cgtactgttc
    47041 gttcttctcg ctgcggcgca tttcgatcag tttattttgc tgccagaaga cattaaacat
    47101 tggcatagtg acgcgttgcg cacgggcccc tttctgagcg gcatcataga aaaatttcag
    47161 ccacgccatt tcatctttac cttcggtata gatctctttt ccgccaggtt tgagtaattc
    47221 cgcaagatcg gcaaacacat caaaatcgtt tcgcgcttca aattgcggag caacagcctg
    47281 cttcatcggc acaatatgct ggttgctgta atcaccggtc atcgtcaagt cattgcgctc
    47341 aaacgatgtg gtgatcggta atacgatatc tgcatgttta gcagctgcgg tccagtagca
    47401 ttcagaaacg acgatcatct ccggtttctg ccatgcttta atcagacgat tagtatcctg
    47461 gtgatgggta aagttaccgc cacccgccca ccagatcatt ttgatattcg gatacgtttg
    47521 ctctttaccg ttatgctgat atttgccgcc aggattttcc agcgcatcga cgatacgggc
    47581 aacaggaatc gccctcattc cgccatcatc agcggcctcg ctggcctgtc cggcaatcgc
    47641 cgcagacatt tccggcagca cgccgccaac acgtgtcggg ttgccaccat ttgagtaatg
    47701 ataagagaaa ccaaaaccgc cgcccggtgt accaatctgc cctaacatcg ccgccagcgt
    47761 caccagcatc cagtgtttct gttcaccata ttgctggcgc tgaattcccc agcctgccat
    47821 cagcattgtg cgattcgccg ccatcagttc agcgagtttg actatctgcg cttccggtac
    47881 gccagtaatt tccgccgccc aggccgcgct cttcggcgta ttatcgctct tacctctcag
    47941 atactcttca aactgcggat acccgatagt gtatttttca aggaacactt tatcgtgttt
    48001 gccttgtgtc atcagggtat gcgcaatccc taacatcagt gccacgtcgg tgcccatatt
    48061 cggcgcgatc caggtggcat tatcgccaaa gaattcgatg gtttcggagc ggataggatc
    48121 aatggcaatc actggtttgc cagatttttt cagctgatgg aagtattcca gcccttgctc
    48181 atcggtactg ctccaggcaa tttttaaagt attcagcggg ttcattcccc acagcaccac
    48241 aacctggctg ttttccagaa tcagcggcca ggaggtctgc tgttcataca cctctacaga
    48301 accgaccaca tgcggcatga tcacctgtgc cgccccggtt gaataatcgc cgctatgccc
    48361 ggaataaccg cccgccaggt tcatataacg ttgcagtaag gtttgcgctt tatgcaacac
    48421 gccagaagag cgccagccgt atgatccggc aaaaatagcc gatggtccgt tagctttgcg
    48481 aatgcgatcg tgttgctcat gaatcagttt taatgcttgt tcccagctca cctgtacata
    48541 ggtatcttcg ccacgacctt tcgtcggttg cagtggatta tcgagatagc tttttctcac
    48601 catcggatgc tgaatacgcg tcgtggtgtg tacctgatcc gccgccgtag actgtaagga
    48661 attcggtatg gttttcgcca gcgcgcctgt tgaagaaaca atcttgccgt ccttcacttc
    48721 tacgttcatc gctccccaac gtcccgcggt gaggatttta ccgcccttct cttctgccca
    48781 tgcggggagc ggtgctgccg atgtcaccac cagcgctcca gcggcaatac cgctgtgttt
    48841 aataaattca cgtcttgtta atgtcataac ttcctccctg atcaacgagg atcactgttt
    48901 ctcggtaata tctttggcgt tgtactggaa ataccgcgtt aaaatgtcca gttcgttttc
    48961 gctcatgctg gttcgcgcac ccattccttt ggcaatggac ggccatgcat tgacggtgta
    49021 atggtcagcg gcaataggcg catggcaacc agcgcaatag gtatcgtcaa gtttttcagc
    49081 gtattgccat agcggtttac ggtccgctaa tgcgggatcg gtaagcgcac cctgtaaaga
    49141 cgcctgacgc cattgattgc cgtattcgtc agcctgccat tccccgttta ccgtgagcgc
    49201 cttgatacct tcttcactta atgtggctag cgccagccgt tgacctgccg ccaggtagag
    49261 cgtgttttca ctgccctgca tttgataacc ctgcaacaga acgatcggct gtttgccact
    49321 ggcatcaacg acggtgagat cggtaccagg attcacggta gccagctcgc ctatgcgaga
    49381 agttttgaaa ggataaatat gtgcgccatt agtcactgaa gtagcggcct gactttccag
    49441 ctcatgcgcc gcgttgtcat ccatttttat ttctggcgga aaatgggcaa tgcctttatg
    49501 acaatcgata caggtttcgc cgcccttttg tgctttgtta tgcattttct gcgcagattc
    49561 actttgcgag gcaatatcca tggcatcaaa agaatggcaa ctacggcacg ttgcagagtc
    49621 agttgctttt aattctttcc atactgtttc ggccatttcc tggcgatgag tttcgaactt
    49681 atcgtcactg tctattttgc cgctaacaaa ttcatgataa atatctttag atgcttttaa
    49741 tttagcaaat aaataatcca tccctgactt tggaatatgg caatctgcac attccgcacg
    49801 tatccctttc tggttcgaaa agtggacagt tccctgatat tcctcaaaag gtttactcat
    49861 cgagtggcag gaaaggcaaa atgctgtatc cgacgtttta tgtaaggctt tttgcgccag
    49921 caataacccg ccgccaccaa ccacaaccgc tatcagcaaa aacaataacc caatgcgttt
    49981 tttccctcgc atagattcct ctaaataaat tccagaacac cgaaatcatt gtataattaa
    50041 atacacaacg aattaaccgc ttgcataact cggcacaact ctgcctgaaa taatcgataa
    50101 agaatatgat ttattacaat gtaatcatta attgctaagg aataacccag ttgctattta
    50161 gaatatatat ttatcagttt tagcaattta aatcccataa tcaatgtgaa tataaaaaat
    50221 attattagca caacgctatg ttcactgtca cccgctcaac agatcacggt tatcgttcgt
    50281 tttttatact gctcagggat aaaaaaaggc ccctgttgaa attgcagggg cctggtacga
    50341 gcaagcatca tattgggcga catgatgcaa cggtaaaaat catttggcct gatggcgttc
    50401 aatgattcct ttcatttcag caaccgccgc cccgtctacg atataacgcg aatactcgtc
    50461 cgcgtgttca tctgatgaca tggacaatcc ttgattacga taacgcatcg gtgaggcttg
    50521 ccacgctccc gcggagctat ggacttccag cactccggca tcgaggaagt ggtgcaggtt
    50581 ttctgcacgg actcctgctc cggccataat gattggagca tcacgatggg caataagttc
    50641 cataattttt gataaacctt gcagtgcgtc tgatttttgc cctgatgtca gcacccgggc
    50701 aatgcccaat tccgcaagat tattgagtgt atataaaggg ttagcgcaca tatcgaaggc
    50761 gcgatgaaaa gtcactgcca gcggaccggc agcagccatt attttttcca ttcgtggcat
    50821 atcgacattc ccgtcaacat cgagaacgcc cgtcaccagt ccaggaaaac ctaattcgcg
    50881 gaccgtgcgc acatcctcaa gaatggcggc aaactcaccg tcgctgtagc aaaaatcacc
    50941 accgcgtggg cgaattatcg gatgcacagg gatcgtcacc cgctggcgca cggatttcag
    51001 tacccccaac gacggcgtta agcccccctc ttttggggct gcgcataatt caactctgtc
    51061 tgcgccgttt tgctgcgccg ttagtgcaca ttccatgctg taacagcaaa tttccagtaa
    51121 tgccattttt gctccttaat tacgctgact gctcgctggc aacgatctct tcaatggacc
    51181 acggatgaaa cttaatagtt gttttgccat cggtgaccgc cagcgtcgga ttcggtaatc
    51241 gctcatgctc accttttggt gaactggttt taactgagat ccccggtagg ctgagtcctt
    51301 catcagaaag caatgccagc gcacgggcat tcagcgtttc tggatcaccc ggcagaacga
    51361 tttcaatatg ttcccaacct tcgtgtgggt aacgtttttc cccgggccac ggtagctcca
    51421 caatagaaaa ctgccagtgc gcaacctgta ccggttcatg caatttaaac agacaaatcg
    51481 gtctgccatt gatcatattt tctgacaaaa gctcgccaca ctgttcaaac ccgcgacgcc
    51541 agcgttcagc agtggcgttt tgatggcaac gcaaagaaat gtgatcggca gtcagcggag
    51601 tgatattcaa ccccagacgg agggaaagtt catctaatgc gtgggtaaat cgcggtaaat
    51661 ccgatgcaat atcctgcagc tcgtcgatag attgccagtt cgccataatc actcttcgtc
    51721 tttcagtaaa agcgttaatt taccctgttg ccctgcgcca accaaccgct gatttcacgc
    51781 cgcttctgat gcaatagtga aaacggcaat acgccacgcg cacgttgctg acgaaaacag
    51841 ccatttgcag tatactcccg ccctaatttc tttaactggt gcgggcaatt tttgctcgct
    51901 tcatcaatgt aaggtattcc ggtgaatatt caggctcttc tctcagaaaa agtccgtcag
    51961 gccatgattg cggcaggcgc gcctgcggat tgcgaaccgc aggttcgtca gtcagcaaaa
    52021 gttcagttcg gcgactatca ggctaacggc atgatggcag ttgctaaaaa actgggtatg
    52081 gcaccgcgac aattagcaga gcaggtgctg actcatctgg atcttaacgg tatcgccagc
    52141 aaagttgaga tcgccggtcc aggctttatc aacattttcc ttgatccggc attcctggct
    52201 gaacatgttc agcaggcgct ggcgtccgat cgtctcggtg ttgctacgcc agaaaaacag
    52261 accattgtgg ttgactactc tgcgccaaac gtggcgaaag agatgcacgt cggtcacctg
    52321 cgctctacca ttattggtga cgcagcagtg cgtactctgg agttcctcgg tcacaaagtg
    52381 attcgcgcaa accacgtcgg cgactggggc actcagttcg gtatgctgat tgcatggctg
    52441 gaaaagcagc agcaggaaaa cgccggtgaa atggagctgg ctgaccttga aggtttctac
    52501 cgcgatgcga aaaagcatta cgacgaagat gaagagtttg ccgagcgtgc acgtaactac
    52561 gtggtaaaac tgcaaagcgg tgacgaatat ttccgcgaga tgtggcgcaa actggtcgac
    52621 atcaccatga cgcagaacca gatcacctac gatcgtctca acgtgacgct gacccgtgat
    52681 gacgtgatgg gcgaaagcct ctacaacccg atgctgccag gaattgtggc ggatctcaaa
    52741 gccaaaggtc tggcagtaga aagcgaaggg gcgaccgtcg tattccttga tgagtttaaa
    52801 aacaaggaag gcgaaccgat gggcgtgatc attcagaaga aagatggcgg ctatctctac
    52861 accaccactg atatcgcctg tgcgaaatat cgttatgaaa cactgcatgc cgatcgcgtg
    52921 ctatattaca tcgactcccg tcagcatcaa cacctgatgc aggcatgggc gatcgtccgt
    52981 aaagcaggct atgtaccgga atccgtaccg ctggaacacc acatgttcgg catgatgctg
    53041 ggtaaagacg gcaaaccgtt caaaacccgc gcgggtggta cagtgaaact ggccgatctg
    53101 ctggatgaag ccctggaacg tgcacgccgt ctggtggcag aaaagaaccc ggatatgcca
    53161 gctgacgagc tggaaaaact ggctaacgcg gttggtattg gtgcggtgaa atatgcggat
    53221 ctctccaaaa accgcaccac ggactacatc ttcgactggg acaacatgct ggcgtttgag
    53281 ggtaataccg cgccatacat gcagtatgct tacacgcgtg tattgtccgt gttccgtaag
    53341 gcagaaatta acgaagagca actggctgca gctccggtaa tcatccgtga agatcgtgaa
    53401 gcgcaactgg cagctcgcct gctgcagttt gaagaaaccc tcaccgtggt tgcccgtgaa
    53461 ggcacgccgc atgtgatgtg tgcttacctg tacgatctgg ctggtctgtt ctctggcttc
    53521 tacgagcact gcccgatcct cagcgcagaa aacgaagaag tgcgtaacag ccgtctgaaa
    53581 ctggcacaac tgacagcgaa gacgctaaag ctgggtctgg atacgctggg tattgagaca
    53641 gtagagcgta tgtaatcgat ttttcgtgag agtaaagcct gatcagggtt acctgatcag
    53701 gcttttttat tgccatctaa atgtattctg aaaatggaca tgccattgtt ttctgactgt
    53761 tggataagag gccagaagcg taatatccgg ccccagggaa acgataacgg ttgaatttaa
    53821 ggaataccgc agtgtttaaa tttcttgtat taacattagg cattatctct tgccaggctt
    53881 acgcagaaga tacggttata gtaaacgacc atgacatttc agccatcaaa gattgttggc
    53941 aaaaaaattc agatgatgat actgacatta acgtgatcaa atcatgcctg cgacaagaat
    54001 acaatctcgt cgatgcgcaa ttaaataaag cctatggtga agcttatcgt tatatagaac
    54061 aagtgccacg cacaggtgta aaaaaacctg ataccgaaca acttaacttg cttaaaaaat
    54121 cacagcgagc ctggctggat tttagggaca aagaatgtga attaatcctt tcaaatgagg
    54181 acgttcagga tttaagtgac ccttattctg aatcagaatg gctctcatgt atgatcatac
    54241 agaccaatac gcgaactcgc cagttgcagc tataccataa ctctgaagat ttttatccaa
    54301 gccctttgac aagaggataa ttcacatctt tttggcatgt tttgttgcaa gctattcctg
    54361 ataaataatt gcaacaagac atcgagcctt tttcactgag ttattaaaca tactcgcgag
    54421 cgcgtaattt ttttgtcctt cagcgataat tcacaatcac ttcattacgt tgtaccttta
    54481 gcggtggaat taaccgccca ccgcctggca cttcccagat aaatcgcaac ggttctgctg
    54541 ccgctatacc ggaaaaggcc acggtggttc cgctctgccc ctctaattcg acacaacgag
    54601 attgcgaaca caagcgaacc cacagtcctg aaggtgtcgg gccgataagc tgataacgcc
    54661 acgctaccag cgtcatcaat cctgaagcag gttgtcgcgt agaaagaggc gcagacgaca
    54721 tcgactcacc gcgatgattt agcgtaattc ctacactact tgcctgccac atcccctccc
    54781 cggcggcttg caccagcagc ggaaacaata atattgctaa taaggttctc attatttgcc
    54841 gccaattgtc gccgtcatgc ggatatgtcg gttatcagac agttccagat tcgacaggac
    54901 cactaactgc ggcaagctgc ggcgcaggaa gcgagacaat aatggtcgca gcgcgtggtt
    54961 caccaacaat actggcggcg cacccagcat ctcctgacgg gatagcgctt cctgagtttg
    55021 cgccagtaaa cgatccgcca gccctggctc cagtcctccc ccgccctgca gcgcctgtag
    55081 taacaaacgt tccagcggtg tatcgaggcc aataacatgg acttcatctt tgccaggaaa
    55141 ccactgctgg gtaatcgccc gtcccaacgc cacgcgcacg acggcggtta attcatgtgg
    55201 atcgctttgg atgggcgcat gttccgccag cgtttcgaga atggtgcgca tatcgcgaat
    55261 cggtactttt tcatcgagga gattttgcag cactttatgc agtgtggtga gcgtgacgac
    55321 gccaggaacg agatcttccg tcagctttgg catctcctgg gcgacgcgat ccaacagctg
    55381 ttgcgcctcc tgacgaccaa acagctctgc ggcatgctgg ctaatgaggt ggttaagatg
    55441 cgttgctacc accgtgctgg cctcaaccac tgtgtacccc tgaatctgcg cctgttcttt
    55501 tagcgcactt tcaatccaga tagcattcag gccaaatgcc ggatcgacgg tcgcctcacc
    55561 aggtaacgtc ccggcagcgg ttccagggtt aatcgccagc cagcgccccg gataagcatc
    55621 accactgcca atctccacgc ctttcatcaa aatgcgatag cgggcaggtt gcagatccat
    55681 attgtcgcga atgtgcacca ctggcggcag aaatcccatc tcctgggcaa atttcttgcg
    55741 gatactgcgt atacggccca acaactcacc atcctgctgg aaatcgacca tcgggatcag
    55801 tcgataaccc acttccattc ccagagaatc ttccagttgt acatcgttcc acgtcgcttc
    55861 gacaacggta ttattctctg ccatttttac cggtttgggt tcggcaggcg ctttttgttc
    55921 gcgtccgcgt atccaccagg ccagccccag caatccggca gtgaacagca aaaataccag
    55981 gttcggcatt ccaggcacca ggccgagtaa accgagcacg gcggcgctta acaacataac
    56041 gcttgggtta ctgaaaagct gattcaccat ctgctcgcca acatcctgat cggtgctgac
    56101 acgcgtaacg atgacccccg cggcggtaga aatcaccagc gccggaattt gtgccaccag
    56161 accgtcgcca atggtcaata gcgtataact ttccgccgcg tgtcccatgc tcatgccatg
    56221 ttgcagcacg ccgaccagca acccgccgac aacgttaatg accatgatga ggatcccggc
    56281 gatggcatcg ccgcgaacaa acttacttgc cccgtccatt gagccgtaaa aatcggcttc
    56341 ctgagtcact tcggagcggc gttttttcgc ctcatcttca ccaatcaatc cagcgttaag
    56401 gtcggcgtca atcgccatct gcttacccgg cataccatcg agaacaaagc gcgcacccac
    56461 ttctgcgata cgcccggcac ctttggtaat gaccataaag ttgatgatca cgagaatgac
    56521 aaacaccacg atacctatag cgaaattgcc accaacgagg aagtgaccga acgcttcgac
    56581 caccttccct gccgccgccg cgccggtatg cccttccatc aaaatgatac gggttgaagc
    56641 cacgttaagt gccagacgca acaacgtagt aaacaacaga atggtcggaa acgcagcaaa
    56701 ctcaagcgtg cgctgggtaa acatcgccac cagcaacacc atgatcgaca aggcaatatt
    56761 gaaggtaaac aacaggtcga gtatgaatgc gggcagtggc agcaccatca tcgacaagat
    56821 caacaggatc aaaatcggtc cggcaaggat ctgccattgt gtcgatttca ggtttgcggg
    56881 caggcgcagc atcgcggcca gattactcat gggtcggttt ctcgttaata aaatccaggg
    56941 cttccggcac cggaagatga gtaggttgta cagggcgctg tccaccagcc agacgccagc
    57001 gtttcagttg ccagacccag gccagcactt ccgccaccgc ggcgtacagt tgacccggga
    57061 tttgttgacc aatctccgca tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg
    57121 tcgggacgtt attttcagcg ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt
    57181 tagcgaccac tttcggtgcg ctcattttgt tttcgtcata ctgcaacgct accgaatagt
    57241 gggtcgggtt attgacaatg acatccgctt tcggcacatc ggccatcatc cgacgccgtg
    57301 cagcagctcg ctgcatctga cggatccgcc ctttaacatg agggtcacct tcgctttgtt
    57361 tgaactcatc acgaatatcc tgccgtgaca tacgcagctt tttcaggtgg ctgaatattt
    57421 ggaaaaagac gtcaaatccc accattggaa tgacaccaag caccaccagc agtgcgcata
    57481 gccctaccaa atccatcgca ttacccatgg cggtaatcgg agactcggcc atcaagcgca
    57541 tcatctgcgg ccagtgatgc cagagaaaaa accccgtcac gctgccaacc aggatggttt
    57601 tcaaaattgc tttaagcaac tccgcgccag tctgagccga gaacatccgt ttaatgcccg
    57661 gtagcgggtt gagtttggaa aacttcggct gcaaggattt gccgctaaat accagccctc
    57721 ccagcatgac cggagaaatg agcgccacca gcaccacgcc gctaatcagc ggcagcagcg
    57781 ccagcatggc ttctctgatc agcagaataa tctgcccgag gatcagattc gggtcattga
    57841 taatactgtg atcaaaatgc agcccagcgg agagcatgcc cgacaatcga cgggccagcg
    57901 acacaccgcc aaaccagata acactaacgc ccaccagcaa aatcagcagt gaggtcagtt
    57961 cacgggaacg cgggatttgc ccctcttccc gcgctttttc tagtcggtga ggtgtggggg
    58021 cttctgtttt gtcgtcgctc tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg
    58081 tgaatgatgc cagagcgcaa agcgttcaat ggtttgagta aggggcaaaa caggcgggat
    58141 ttagggcttt tgctgccaca catcaagcat agtgtgcgtt tgtcggatgc ggcgtcatcg
    58201 ccttatcaga ccgcctgata tgacgtggtc acgccacatc aggcaataca aatcaaaatc
    58261 caagactatc caacaaatcg tccacctgat cctgactggc taccacaccg gctttgctgg
    58321 tatcgacctg aggtccatta agcaaactct ggttttcacg ttttggacgc gactcctgtt
    58381 ccgggatgtt ttccaacagc accatcagca actggcgttc gatctcctga atgacatcca
    58441 tcatccgctt aatgacctgc ccggtgagat cctgaaaatc ctgcgccatc atgatttcca
    58501 gcagttgcgc gttagtaaag ctggtatgcg cgggtacatc tgccagaaat tgtcgtgtat
    58561 ctgttaccag ttcacgggcg tcggcaaggt caatcggatc ggcaaaccag tcatcccaac
    58621 gttgggttaa cgcttttgct gatttctcca tttgatcctg atgcggttgt gacgcctcaa
    58681 cactgttcag cgcccgctcc gcagcctggg cggtcatctg cacaacatag tacaaacgat
    58741 cgcgcgcatc ggggatggct tccgccgctt cggcaatggc ctgatccagc cccagttccc
    58801 gcaaactgtc gcgcagcata cgcgtcaggc tgccgatgcg cgcaatgata tcgccagctg
    58861 aatgctcgtc agcaggtttg attgatggtt gcatcatagt cgcatcctca catgcccagt
    58921 ttctcaaaga ttttgttgag tttttcctcc agcgtcgcgg cggtaaatgg cttcaccaca
    58981 tagccactgg cccccgcttg cgccgcagca atgatgttct ctttcttcgc ttctgcagtc
    59041 accattaaca ctggcaatgc cgacatcgcg ccatccgcac gaattgtttt cagcaattcc
    59101 aggccatcca tattgggcat gttccagtcg gagataacaa atccataacc gcctgcctgc
    59161 aacttattga gagcgtcgac gccatcttcc gcttcctcaa cattattgaa tcccagctct
    59221 ttcagcaggt tacgcactat gcgtcgcatg gtggaaaagt catccacaac caaaaattta
    59281 agttctttat ccgccatttc acactcctga tttaaatacg tatcgcctgt ccggcactaa
    59341 tttttgccaa catttgctgg cttacctggc taagatcgac cacttcgcag acaccaccca
    59401 tattgatggc ctcgcgcggc atgccgaaca ccacgcaact tgcttcgttt tgcgcaaggg
    59461 tccatgcccc cgcctgacgc atcgccaaca ttcccgccgc gccgtcgttg cccataccgg
    59521 tcaggatcac cccaaccgca ttacgccccg cctgtttggc gacagaatgg aacaacacat
    59581 ctaccgaagg ccgatgacgg ttaaccgccg ggccatcgtg aattttgatt tggtaatttg
    59641 cgccactacg cgacagctcc atatgccgat cgcccggcgc gatataggca tgtcccggca
    59701 aaacacgttc tccgtcttcg gcttctttaa ccccgatctg acaaagctta ttaagtctgt
    59761 cggcaaaaga gcgggtgaaa ccaggcggca tatgctgggt aattaacagt gccgggctgg
    59821 aaagcggcaa cggttgcagt acgtgacgaa ttgcctcagt tccacccgtt gaagcaccaa
    59881 tcgcaatcag tttttcagaa ctcaacaacg gccctgcctt cagcgttgtc ggtgccgaca
    59941 atggcttatg tgctgcaagg ctcgcctttg ctgctgtacg caccttttca gcaatcattt
    60001 cgctatacgc cagcattcct tcgcgaatac ccagttgcgg tttggtgaca aaatctatcg
    60061 cccccagctc cagcgcgcgc agcgtgactt ctgacccttt gccggtcaag gaagaaacca
    60121 tcacaacggg cattggacgc aaacgcatta atttttcgag gaaatccagt ccgtccatcc
    60181 gcggcatttc aacatccagc gtcagcacat cgggattgaa tttcttaatc aagtcacgcg
    60241 cgaccagcgg atcaggcgcg gtcgccacca tttccatgtc gctatggctg ttgatgattt
    60301 ctgtcatgat ctggcgcatc agtgccgaat catcgacaga taacaccctg attttgctca
    60361 tcgttaatcc ttacttagcg catacaccgt ctgaccacgc agcgtgaagc ggcgctcaag
    60421 gtggctaaag ttttcagagt gacccgcaaa cagcaatccg tcgggtttaa ggagcggaac
    60481 aaagcggcgc aaaatctcct gctgggtatt ttgatcgaag tagatcatga cgttacgaca
    60541 gaagatcgca tcaaacggcc ccggcacggt gtactgtttc gccagtagat tcagcggggc
    60601 aaaatcaaca tagttcgcca gctcctgacg cacgcgtacc agcccttcat gcggtcccgt
    60661 ccctcgcatg aaataccgtt gcagttgctg cggcgtcagg tttttcaact cttcatggcg
    60721 ataaataccg cttctggctt tttccagcac ttcggtgtcg atatcactgg caaacacttt
    60781 ccagcgtccg ggcgcggtgc ccaatgtgtc agccagcgtc atcgcaatgc tgtacggctc
    60841 ttcgccggtc gaagccgccg cgctccatac gcgatactcg ccagaacggc gacgtgcgtg
    60901 atccgcgagc agagggaaat ggtgtgcctc acggaaaaat gccgtcagat tcgtggtcag
    60961 cgaattgata aacgcctgcc actcaccgct gtgctgatta gattccagca agttcagata
    61021 atgaccgaaa tccgtcagtc ccagcgaacg caaacgacga accagtcggt tgtaaaccat
    61081 gtcgcgttta tggtcagcca gaacgatccc ggctcgttga tagatcaatt gacttatccg
    61141 ccgaaaatgc gcgtcggaaa gcgccaggcg ctcggtcatc tgtaacaata aagacgtttg
    61201 cccacagggc agagatgaag tcatagcgcc ttctcaatca cttcaggata ccactggcgc
    61261 aatttgtaac tgcgccgact catgtcgtgc tacttcatgt tcttcaaggg taaataccgc
    61321 cacacgcgac gaaagatggt cggcctgatt cgccagttgt tccgttgcca ccgccgcttc
    61381 ttctaccagc gaggcgttct gctgagtcac ctgatccatc tggctgacag cctgtgcaac
    61441 ctgctcaatc ccccgctgtt gttcttccga cgcagaggca atttctccca taatgtcgtt
    61501 cacgcgggtc accgaactga caatatcgat catggtcgcg gcggcgttat tcaccagttt
    61561 cgaaccctgc tggacacgat tgactgactc ttcgatcagc cctttgatct cttttgctgc
    61621 ctgcgcgcta cggctggcaa gattgcgcac ttcacctgcc actaccgcaa aaccacgccc
    61681 ctgctctccg gcgcgagccg cttccactgc cgcattcagg gccagaatat tggtctggaa
    61741 agcaattccg tcgataacgc tgataatgtc gccaattttt tgcgagctgg tggcgatctc
    61801 ctgcatggtg tgagtcatgg tactgacctg aacaccgccc gcctgcgccg ttgtcgcggc
    61861 attttttgcc agttccgacg cctgtcgtgc gttatcggcg ttttgcccta ccgtggcggt
    61921 taattgctcc atactggcgg ccgtttgtgc cagcgatgcc gcctgctgtt cggtacgact
    61981 tgagagatcg ttattgcctg cgacaatctc cgcgatacca atgtgcatct cctggcttcc
    62041 cttacgcaca tcacttaccg tcccacgcaa agcctgctgc atggtcttca gactggcaaa
    62101 aatggcggtg atctcattac gaccatatac cgcaatcgga cgcgccagat tacccgcagc
    62161 aatgctgtca aaatggctac cgataatggc cagtggttga acaatcatct tgcgcgtcca
    62221 ccacagcgca ctgctgatgt agatcgccgc aacaataatc atgctgataa acaccagcgc
    62281 cgaaatctga tagttacgct ggctttgcgc actggcggct tccagcacat ggttgatctc
    62341 cagctgccag gcctcaaagt taacgtcaaa cgcattctgt gattcctgca ccggcgcagt
    62401 gaggaaatcc gaaagttgat tactttcaag ccaggtcgcc tgatgttcga gatcgttgtg
    62461 ccagcgggca aaacttttct ccgtctcttt ttgcaacgcc ctgacgtgct cgttgcccgc
    62521 agtcatcgcc ataaaacttt taaacagcgt ggtggattgc gtcagactgg cgcgcgccgt
    62581 cgtcatcaac gttttaatgt catccgccgg atagctaagc gccgtcagag tgcccgcttt
    62641 gttcagcgcg gtactggcct gtaacattac cgcccgagtt tgcgctaatg ccgcacgttg
    62701 ctgattgctc tgctcgacct gattcagtcg ttgcaaatcg tcgcgaaatg cccaaaaaga
    62761 catgccgtta ctgccaatct gcaagatccc gcagagaatc aaaattaaaa acagcgtggt
    62821 cgaaattcga atacgattaa acatcaacgc tccccatcag gcggcaatga ccgcgttagt
    62881 aaatactcgt caaaatgttt cccagtttgg atcttgttca gcaattcgca gtcgtggctg
    62941 tgccggtggt tgctcactgg caggacggga tggtgtttgc ggtttattgg tgagtgggct
    63001 ggctgccaga cggaacgcgg aaaccgcttg cgttaaacga ctcgcctgtt cttccagcgc
    63061 agcggcggcg gcagctgatt cctgcaccag cgatgcgttc tgttgcgtga cgcgatccat
    63121 ttccgaaacc gccaatgcga cttgatcgat gccacggctc tgttcatccg atgccgatgc
    63181 aatctcgccc ataatgtcag tcacgcgagt gacagcattg acgatattgt tcattgtttc
    63241 cccggcgctt tcgaccagca ccgaaccggt atcaacgcgt gagacggagt cttcaatgag
    63301 ggctttgatc tcttttgccg cctgggcgct acgactggca agattacgca cttcacccgc
    63361 caccacggca aaaccacggc cctgttcacc cgcacgcgcg gcttcaaccg cggcattcag
    63421 cgcgaggata ttagtctgga aggcaatacc gtcgataacg ctgataatgt cggcaatttt
    63481 cttcgaacta tcggcgatct catgcatcgt tttcactacg ccatccacca ctttgccgcc
    63541 gtgctgggcg gtgtcggagg cactttgcgc cagttgcgag gcctggcggg cgttatcggc
    63601 gttttgcttc actgtcgcgg tgagctgctc catgctggcg gcagtttctt ccagcgcgga
    63661 tgcctgctgt tcagtacggg aggaaagatc ggtgttgccc gccgcaattt cacgggtacc
    63721 ggcatagatg gcatctgaac cttcgcggac atgagtgacg gtgtcagtca aagagcgttg
    63781 catatgtgaa acgctctgcg ccaggtcgcc catttcactg cgcccgtcaa tggtcagggt
    63841 attcgccagg ttaccaccgg cgatttcgcg aatgtgagca ataatttttg ccagcggagt
    63901 aagcaacata cggcgaatgc cgtaccacgc caccagcaga atcaatacca ccaccagcgc
    63961 gataaccgcc agttgccact gggcaaatcg gtaatcatct gcgttgtcag tgacgatatc
    64021 gcgatacagt ttttcactgc tgagggcgta ctgagcaaac gcttcgccca ttgcattttg
    64081 cattccctgg gttggctgag cgaaataagc tccagtattg ccataatcta gataatcaat
    64141 cagttcagtt aacgctgtgt aatagttttt atatttttca tcaatattac gactggtagc
    64201 gaccatttca ggtaacggtg ccatgctttt gaatttttta taatgcgtcg ctgcctgcgc
    64261 caatgttttc ctggcgctat cgagcaattc aactttggcg ttactttgtt gattggagga
    64321 atccatcatc atccgtaccg ctgaacgact caggttaatg cgcgtttgca gcattaaatc
    64381 ccaggttgac gtcagctcgc cctgctgttc ccgtaattga ttggaaacca caaagctctt
    64441 ctggctatgg tgaagggaag aaaaaaacag actgccggaa ataagctgta acagtgcgaa
    64501 tacccccagc accattacca acagcgtgac tacgcggata cggttaatca taaggcacct
    64561 tcctgaaaac aagttgatct cgttatcggc aaggaggggg gaaactttat tgctgatgcc
    64621 acccgccgcg aaattgaaat aaaaaacccg atgcgcagat catcgggttc atttcaattg
    64681 aggaaatcgg gagaattacg ccacttctga cgccgcgcta tctaacagcg ccatctcttc
    64741 gctgttcagc agtttttcga tgttcaccag aatcaacatc cggtcgccca gtgcgcccag
    64801 tccagtgaga tattctgttg aaagcgtcac ggcaaattcc ggtgccggac gaatttgctc
    64861 cgccgtcaat gaaagcacgt ctgagacgcc gtcaaccacg atgccgacca cccgctgtcc
    64921 gagattcagg acgataacta ccgtgttgtc gttatagtcc acatccacct ggctgaactt
    64981 aattcgtaag tcaacaatcg gcacaataac cccgcgcaga ttcgtgacgc ctttgataaa
    65041 cgctggcgtg ttcgcaatcc gtgttacctg atcgtagcca cggatctcct gcactttcag
    65101 gatatcaata ccgtactctt catcaccaag ggtaaatacc agaaattcct ggcctgacgg
    65161 ctcgctggcc agctttgtta cattcgtcat accggtcata ttgttacctt tttactcatt
    65221 caggcggcgg tgttcgccat acgttgttcg cggtttatcg cctgcaaggc ggagacatca
    65281 acaatcagtg ccacgctgcc gtcgccaaga atggtcgcag cagaaatgcc ggggactttg
    65341 cgatagttac tttcaaggtt tttaaccaca acctggtgct gaccaattaa ttgatccacc
    65401 agcaaggcgt agcggcgacc gccactttgt aagatcacca caattccctg ggtggcttcg
    65461 gttttcgcgc ccgcgacgtt gaacactttc cacagttcga cgatgggcag atattcaccc
    65521 cgcacttcca gcaccctctc gccgccggcc agtggatgca gatcggcttc acggggttgc
    65581 agtgattcca taacagcatt cagcggcaga atgaaaactt catccgcaac gcgtacggac
    65641 atgccgtcga ggatggccag cgtcagcggc agtaaaatgc ggatcgtagt gccagtaccc
    65701 tgcttcgact ggatttcgac atgaccgccc atctcctgga tattacgttt aacgacgtcc
    65761 atgccgacgc cgcgcccgga gacgtcggtg acctgctctg ccgtggagaa gcctggtgca
    65821 aatatcagca tcgcgacttc gtcgtcgctc atgttttcgc tgacagtcaa accttgcgag
    65881 gccgcttttg ccagaattcg ctcacggttt agccccgccc catcgtcggt cacttcaatg
    65941 caaatgttgc cgccctgatg ttcggcagac agaattaaat ttccgacgct gtttttacct
    66001 gcggcgagcc gtttttctgg cagttcaata ccgtgatcga ggctattgcg taccaggtgg
    66061 gtcagcgggt cgataatgcg ttctatcagg cttttgtcga gttcagtaga actgcccacc
    66121 agcgtcagtt ctacctgctt gccgagtttt cccgccagat cacgcaccag ccggggatag
    66181 cgactaaaaa catattccat cggcatcatg cgaatcgaca tcactgattc ctgcaaatca
    66241 cgggcgttac gttgtaactg ccccatgctg gttatcaaat caccatgatt aaccgggtcc
    66301 agttcgctgg aacgctgggc aagcatggac tgggtgataa ccagctcgcc gacgaggtta
    66361 attaattgat caaccttttc tagcgctaca cggatgctgg tggattcatt gctgcgcgtc
    66421 gttttttccc gctccacgcg gccggttggc gcttgttcgg ctgccagttt aagcactggt
    66481 ggggtggata tttttggcga gacttctact gtttcaaagg taatctgatc ggcttcaatc
    66541 acaaaacaga gtaccgctgt gatgtcatct tcggcgatgt cgcccggtaa tattgccgag
    66601 agcgaatccg cccctttcac cacgtcagtt aacgttgtca gatgtcccag ttcttcttcc
    66661 agcaggtcga cttccccggc cttcaggcgc gaaaggataa ttcgtcgcgg cgactgactg
    66721 cgactctgct catcttgcgg ttcacttttg gcaaccacac ttaatcgggt cactgcggat
    66781 ggcgtttcgc ctttcgcttc taatgccagt tgacgcaagg cctggcagat ataatcgaag
    66841 ctggcggcat ccggctcttg cgactgttta taagcgtcga gctgttcttg catgatgtcc
    66901 ttcgtttcca aaaacagatt gataatgtcg gtgttgagtt gcatctcacc tcgtctggct
    66961 tcatcgagca ggttttccat cagatgcgtg gtttcctgca aaacgctgaa gccaaaagtt
    67021 cctgcccctc ctttgatcga gtgggcagcc cgaaagatgg cattcaattg ttcggcatct
    67081 ggcgcttccg gctgcaaaac cagcagatgc tgctccatat cagccaacag ttcgtccgct
    67141 tcatcaaaaa atgtctgata aaaatcgctt atatccatgc tcacgctgtc acctcggttc
    67201 ggctgatggc attgtgggaa cactgacctg tggtgcaacc tcaggttttt ccagggcgct
    67261 tactggctca ttctggcttt cggcgttttc atgcaaaatg gcctgttcgg cttgtttgtt
    67321 cagtaccagc aggctgatgc gacggttgac ggcatcatca ggtccgcgat cgcttaagcg
    67381 catcgttgcc gccatgccga cgacacgtaa cactttgccg ctatccaacc ctccgaccat
    67441 cagttcgcgg cgggatgcat tggcccgatc ggcagaaagc tcccagttgc tgtaaccttt
    67501 ctcaccactg gcgtatggga aatcatcggt atgacccgaa aggctaatac ggttgggaat
    67561 accattcaga acgggcgcga tggcgcgcag aatgtcgcgc atatagggtt cgacatcggc
    67621 actgccggtt ttaaacatcg ggcgattctg gctatcgatg atctgaatac gtagaccttc
    67681 ctggaccaga tcgattttga gatgtggacg taacgcccgc agtttcggat cggactctat
    67741 caactggtcg agatcacccc gcaatttccg caatcgactt tgctccatgc gttttttcaa
    67801 ctcttcgatg ttcggctgct tattcacttc cccctggctt tgggtgtaat catcaccacc
    67861 gccgggaatt gggctttcac tattagaaat gcgatcgccg cccgtaaccg cagtcgccag
    67921 tggagtccgg aagtactccg caatctgaat cagctctttt gggctggaga tggagatcag
    67981 ccacatcacc agaaaaaagg ccatcatcgc agtcataaag tcggcataag caatcttcca
    68041 cgatccatgt gctgccccgt ggcttttggc tttgcgtcgt ttgacgacaa taatcggatg
    68101 cgcttgattc ttcatgcttc ctcggttgtc gtctgttgtt gcggattttt caccgcacgc
    68161 acatgctctt ccagttcaat aaacgacgga cgttcgctgg aatagagcgt tttgcgacca
    68221 aactcaacgg cgataggcgg tgcgtaaccg ttcagattag aaagcagagt gactttgacg
    68281 cactgcatca ttttgctggt ttcggcgctt ttctgacgta aaacactcgc taatggggaa
    68341 ataaatccgt aagccaataa aatgccgagg aaagtcccca ccatcgcatg tgcgataagc
    68401 gcacccagct cggcggcagg acgatcggct gaacctaacg cgtgaacgac ccccattaca
    68461 gccgcgacaa taccaaacgc cggaagtgag tccccgacca gcgccagact gttcgccggg
    68521 acttctgcct cgctttcgtg cgtctcaatc tcttcatcca tcagggcttc gatttcgaag
    68581 gtgttcatat gaccgctgat aatcagacgc agataatcga cgataaaatc aagcatgacg
    68641 ctatccgcga ggatgcgtgg gtagctgacg aagatctcgc tctcacgggg attttcaata
    68701 tcacgttcca gcgaaaacat ccccatctgc cgcgatttcg ccatcaaccg ataaagcaga
    68761 gccagcagat ccatatacat tgctttggtg tatttggagc gacgaaacag caacggcagc
    68821 gccttcagcg tgcctttaat cgctttgcca ttattgccga cgataaacga cccaatccct
    68881 gcaccggcaa taatcaccag ttcagcgggt tgatagagtg ctccaaggct tccaccggtc
    68941 atcaaataac cgccgaaaac tgtaccgaga acaaccaggt aacctaataa gataagcacg
    69001 acatcatcct tccactgttg accatgacag gatgttcagt cgtcaggcgt taacgcgcga
    69061 ttggggcaaa aaaaagcagc ggtacgtcgt taccgctgct ggaatgttgc gcctcaccgt
    69121 atcagttaaa cagcctgtac tctctgttca tccagcagtt gtgggataat atcggcagga
    69181 ttctgggaaa gtttacgtct ttttactgcc cgggatggcg gttgacataa gctgcaggca
    69241 aagctgccaa caggctggtg agcgtgggta ataaaattgc cgccgcagca gttgcagctg
    69301 gaaagttgca gtaatccact ttcaacaaac cgcaccaatg tccaggcacg ggttaatgcc
    69361 agcagtggtc cttcttctgc ttgtgggcac tgttcaaggt ataaacggta ggctttgatc
    69421 accgcatcga cgccattaca caaaccggtt ttcagtaaaa actgccatgc attacagaac
    69481 atcgaagcat gaacgttttg ttcccaggtc ataaaccagt cggttgagaa tggcagcatg
    69541 cctttcggcg gtgggcttcc gcgcagttct ttataaagtt ttatcaggcg tccgcgactt
    69601 aactgtgttt cgctttccag catctgcaaa cgagcgccca gggtgatcaa ttccattgcc
    69661 agctgaatat cccgcgcttc ctgaacaatg cttttttcac tcatgatcag gcccttttct
    69721 tgcgcagcgc ttcttcaggc tgattaacat cattcagcaa gcgtgttgag agcatgatgc
    69781 cggtatgaat ttgctggaga tcgtcaacgc gggaatcttg cgtcaactga gtaatcgtct
    69841 ggtggctgtc aaaacggaag tgacaaacca gttgattggt ttctgccagc ttaaccattt
    69901 gcggaagagt cagtgccgct aacgttgtcg ccatttcttc atttatgccg agacgaaaca
    69961 tagcggacgc tttgtcctga acaatcaaac gctgtgcaag tagtaaatat gacaagttga
    70021 tgtcataaat gtgtttcagc aactcggagg tatgcattat tcccacccag aataaccaac
    70081 tttattttta tgcggtttca ccgcaccccg tgatgtcgcc gggaagcccc ggtaaaaaat
    70141 aattagcatt agaatagttg cgataagctg caataagcag aaccaccttt ttggtttaat
    70201 atgtccttac aaatagaaat gggtctttac acttatctaa gatttttcct aaatcgacgc
    70261 aactgtactc gtcactacac gcacatacaa cggagggggg ctgcgatttt caataatgtg
    70321 tgatgcagat cacacaaaac actcaattac ttaacataaa tgtataagtc atacttttgt
    70381 tttgggtgta tttcaatctg ttaaaaagtt tttcgctacg ctagcaagca aaaatgaaac
    70441 aggaataatc gaaatgggat gttgcacaca gttaaaataa ctcaccgtaa ataatcatct
    70501 gctataaata atcactttca tgcaatacca gataagctat ttttaaacag acacttaccg
    70561 cacaacaaac taattaaata caaagaaagg caacaatcaa cttaatgtta ataaaatgtt
    70621 ttgcatatat taaccacttg tttaatgcgg agaatcagta cactattttc ttataattac
    70681 atttgaaatt atatgatcac cgggagtgat tgattaattg tctttactaa taatcagact
    70741 aatatttacc tgtttgaccg agttgggatt gcgtcgtttc tccattagga gtaaagcttt
    70801 aatgtcacct gaagttcaca gaataaggaa caggttatga gctatagcaa tattcttgtc
    70861 gctgttgcgg ttacaccgga aagtcagcaa ctgctggcaa aagcggtatc tatcgccagg
    70921 ccagtaaagg gagacatcag tttaattact ctcgcttccg acccggaaat gtacaatcaa
    70981 ttagccgcgc cgatgcttga agatttacgc agcgtgatgc aagaagaaac gcaaagcttt
    71041 cttgataagt taattcagga tgcggggtat cccgttgaca agacgtttat tgcctacggt
    71101 gagttaagcg aacatattct ggaagtatgt cacaaacatc atttcgattt ggtgatttgc
    71161 ggtaatcaca atcacagttt cttttcgcga gcatcctgct cggcgaaaag agtgattgcc
    71221 tcaagtgagg tcgatgtgct gttagttcca cttacgggag attaaccgct cctacgcaag
    71281 ctttggaaag gtagcaactt tatcgcgctg ctggctttcc gcgcttcgcg gaactatctg
    71341 ctttaggtcg ctaatgaagc actcctgcca gtggttaata tcgtttttca cgataacgtc
    71401 cagcatttct gcatgacggg aaatacgttc cgccagcgac atagtcaatg cacgatccag
    71461 cgcagctgca acttcgtcac gatcgtaggg gttaacaatt aacgccgacg ttaactcgtt
    71521 tgccgctccc gcaaattgcg aaagaacaag aacgcccggg ttggctgggt cctgagcagc
    71581 aacatactct tttgctacca ggttcatccc gtcacgcagt ggcgtcacta agcccacgtc
    71641 agagtagcgg aatattttca tcagtaattt acggtcaaaa tgctgattca aataataaag
    71701 cggcgtccag cctaattgcc cgtatttacc attaattcgt ccagcttcat tttcgagctg
    71761 atgacgaata tcctgatagg cttgcacatc accacgcgac gttggtgcaa tctgggtata
    71821 acgaatttta ccatgatgct gcggatattt ttccagcaac gcttcatagg cgagaaaacg
    71881 ctctggcaaa cctttggaat aatccagccg ttcgacagaa aagatatttt gtacgttttt
    71941 cagttccgct ttaagttgcg ccagttttgg cggcagtggc ccggcagcct gtttggctat
    72001 ttctttcggt tcaatgccga tcgggtagac ttctgttcga aatgcttttc cccaggctgt
    72061 atggcttttt gcgctacgtg tcgtgacgcg ggtcaggtta gaaaggcaat ccaggaacgc
    72121 cagacgatcg ttttctgtct ggaaacccag caaatcataa tcacaaagct gttcaagcaa
    72181 ggtgtcatat gtcggcagcg cgttgaagat ttccggtgtc gggaaaggaa tatgcaagaa
    72241 gaaaccaatg cgattattca ctccccgttt gcgtaattca tgcgcaaatg gcaacaggtg
    72301 ataatcgtgg atccagataa tgtcatcgtc ttgcaacagc ggcagtaatt tatctgccag
    72361 caacgcattt acgcgtagat agccgtccca ggcaggacgc tgaaattgca ccagatcgag
    72421 ccgataatga aaagcgggcc agagaacggc attggagaat tggttgtagt attcgtcaag
    72481 gtcctgttcg ctgaggttaa aagaggccca cgtaatgtta ccttttttca ccttttttag
    72541 cggctgatcc tcattccctg tttcaccact ccagccaaac cacagtccgc ctgcggcttt
    72601 cagtgccccc agtatgccaa cggcaaggcc accggcactg gcggcgtgct cgtctggtgg
    72661 tgcaatccgg ttagatacta cgactaaacg actcatagtc atcactcctg ttattttctc
    72721 ttttttgttg taatgcggtg gttatcattt caagccagct ccagacatcc ggcacacccg
    72781 ccagtcgcca tgatgcctga gttgcacctg tgccaatttt tactgacatt ccgcccagcc
    72841 gattaacgac tgcgaagcca gattcatcgg ttaaatcatc gcccagaaat acgggcgttc
    72901 gcccgataaa gggagcttcc tgcataaagg ctgcaatcgc ttcaccttta ctggtacctc
    72961 tcggtttgat ctcgacaaca cactttccct gctgtaacgc catttgtggc cagatctgag
    73021 taatacgttg cgctaatgtc attaatgcgt cttcatgctg cggagcctga cgataatgca
    73081 gcgcaaaagc catccctttc gtctccagct ccgcaccggg atactgggcg atgaccgtat
    73141 gcagttgcac gctaatatca cgcgcaatcg catccggcag atgaacgata tgtgttttac
    73201 cattgatgtc acggcgctcc gccccatgca cgcccgctaa cgggaagcga taaggttttg
    73261 ccagtgcgtc aagctccacc attgagcgcc ctgatatcaa tgccaatgca ccatcacttg
    73321 cggttgccag tagctgtagt ccttgcagaa tattgtcagg cacgacgacc tgatcgggat
    73381 gcggtttgat ttccgccagc gttccatcga gatcaaaaaa ccaggcatat ttcgcggata
    73441 gttcaggggt ttcggttaac ggttctgtca cccggttctc ctccttctct tttcttattg
    73501 tgttgcgcag aacgcgcttt acagacatgt aagtatagac agtgtgacgg gggtcgccat
    73561 ttgcaatgaa gaacagccaa ccgttgtggc ggctggctgt ggtgggaaaa aacgttaaat
    73621 tgttgtggaa aaaagcatca gacagtgcgt ttcgcttttt gcttgtaacg gtcgaagatc
    73681 accgctgcca gcaggattaa gccgcgaacc acgtactgcg cgaaaggaga aatattaagc
    73741 aggttcatgg cgttttccac ggtgcctaaa attaagatac ccgccaccac atatgagatt
    73801 tttccgatgc cacctttcag agaaacgcca cctaaaacgc aggcggagat aacaataagc
    73861 tcataaccaa tcgacgtcat tggctgccca ctagtcatac gtgaagccag aataattccg
    73921 gctatcgcag ataccaggcc agagagaaca aagataataa ttttggtgcg aacaaccggt
    73981 acacccgcca gacgcgcagc ctcttcgttc ccgccaatcg ccagggtgtt acgaccaaag
    74041 gtggttttat tcagcaacaa accaaagata atcagacacg cgacggtgag ccagattggc
    74101 gcaggcagac cgaaccagtt agcgtaacca agggcaaaga agctttcatc ttcgataccg
    74161 accgctttac cgtctgaaat gatgtacgcc agacctcgaa caatctgcat cgttgccagt
    74221 gttgtgatca gagcatttat tttcagtttg gcgataacaa agccattgac caggccacag
    74281 agaatgccaa gcagcaaccc cgctgccacg ccaatccaca ggctttcagt caggttgata
    74341 accaccgccg tggtgacacc cgcacaggca attacggagg cgacagaaag gtcaaagtca
    74401 ccggaagcaa ggcagaacaa catgccacac gccaccatcc ccgacatgga aattgccagg
    74461 cccaaccctt tcatattaat gaaggtggca aaatttggga caaaaatggc acaggcgata
    74521 aagagcaccg caaacaccac cagcatgccg tactgatccc agatacgccc gaagctgaat
    74581 gacgacttag gtgcgccaga ccccgatgta gaaacagaag acatcatact ctccttactc
    74641 aggcaactgc ctggctgact ttaggcatcg caaggctcag tgcctgacgc tcatctgcct
    74701 gctcgtgtaa caattcaccg gcgatttcac cttcccgcat caccacaatc cggtcggcaa
    74761 cgccgaggac ttcaggtaag tcgctggagg caaacagcac cgccacaccc tgcgccgcca
    74821 gcgcataaat cacgttgtaa atttcatgct tagcaccaac atcaatgccg cgcgtaggtt
    74881 catccagcaa aatgaccttc atctcttccg ataaccagcg gcccagaatg gctttttgct
    74941 gatttccgcc tgagagattc atgatcagtt gctcagcgcc cggcgttttg atgttgagcg
    75001 aacgaatgtg gtgatcggca ttgttttctt cccaaccgtt gttgattaca caaccgccga
    75061 gcacatgttt atgtctggca ctgatgttga tattgtcgcg aacggagtgc acgggaataa
    75121 tgccttccgc tttgcgatct tccgggcaga gcatcatgcc tgcggcaatg gcgtggctcg
    75181 gtttacgaat atcgatcggc tgttggtcga tataaacctg accggcggtg atttgcgtcc
    75241 cgccaaacaa gcctttcatt aattcgctac gccccgcccc taccagacca aacagcccaa
    75301 caatttcacc actgcgaacc gccagactta ttggcgtacg cacgcctggt gctttcacag
    75361 catcaagacg caggcgctcc tcgccataac tacgaggttg ccagccgtag atatcgccaa
    75421 tgtcgcgccc gaccatcgcc tgcaccagcg cgtcgtggtc aacctgctgc atatcggtaa
    75481 aggttttgac ataacgtcca tctttaaaga cagtgatggc atcgctgagg gcaaatattt
    75541 cttccatacg gtgagaaacg tataagatca cccgcccctc ttttcgcagt tcacgaataa
    75601 cgcggaaaag attgtcgatt tcgcgggcag aaagggagct ggttggctca tcaaaggcga
    75661 taattttagc gttacgcgcc agcgccttgg cgatttcaac catctgccac tgaccaatgg
    75721 agagatattt cagcggcgtg tccgggtcaa tatccatacc aagatgttta agttgtaaac
    75781 ccgcctcata attcagcaat gagcgattca caatgccgcc tttatgcggc agctggccga
    75841 gatagatgtt ttccgcgacg gtcatttccg gcacgagatg tagttcctgg taaataatcg
    75901 ccacacccgc gttaagtgct gcggtcgtgt cggaaaagga catttcctgc ccattaatca
    75961 ctacagaacc cgtggttggc gcatagttgc cgctgaggat ttttaagaga gttgattttc
    76021 ctgcgccatt ttcacccatc aacgcatgaa cctgaccggc atagcagtca aaactaatat
    76081 ccgtcagcgc cttaacgccg ggaaacgttt taccgatgcc gcgaaatgag agatacgggg
    76141 tagactgttg cataacgtct ccgtgaatca ctggtcgttc acaccccctc tgcatcatgc
    76201 agaggggaat ttttccggca aattacttac cgcctaaacc ttttttctcc agttcttctt
    76261 taaagttgtc acgcgtgatc agtaccacgt cggtaacttc ggtaaatttt ggcggttcaa
    76321 cgtcttttgc tacccagttg taaagcattt cgctggattt atagccatgt acgtccgggc
    76381 ttggcagcag ggaaccgtag aagccggttg cctgtgcttt agacagttcg ctcaccgcat
    76441 ccacaccgtt aatgccaatg ccgatgatat cggccgcttt aaagccctga ccttccgtcg
    76501 cgcgtacgcc gcccagcacg gtgctgtcgt tcataccgac gatcagccaa tgtttaactt
    76561 ccggatgttg aaccagcatt gagttggcag cgtcaaatgc ccccgggatg tcgttagatt
    76621 tggtaggtac ctgataaatt tgtttttccg ggaatccggc cgctttcagc gcatccatag
    76681 atcccgtagt acggcggcgg gcggtatcca gttcgttggc ggtaatcgcc atcaccgcgc
    76741 tttctttgac atcccagcca cgtttctgca tctctttata cagttcctgg ccctgacgtt
    76801 cgccaatttt agtcgccgcc atcatcacca gcggaacggt atccattggc ttacctttgg
    76861 cgttaacaaa ctggtcatcc acggcaatga ctttcatatc gtagccacgc gctttcgcga
    76921 cgatggcaga gccgagtttg gggtccggag tacaaataac gaaacctttt gcgccactgg
    76981 cagccaggct gtcgatcgcg ttcaatgttt tttcgccatc cggcacggca atcttaataa
    77041 cctcaaaccc taaatccttc ccggctttat cggcaaactt ccattcggtc tggaaccacg
    77101 gctcttccgg ttgcttcacc agaaaaccga gcttcaggtt ctccgccata gcggattgtg
    77161 acataacggc tgccagacca atggctgcca gggctttagt aaatttgtgc atggttctct
    77221 ccagctttag tgtcgttttg tgtagggcaa aaacgaatga cattcgttaa attaatcgga
    77281 aaacaaagca ttacctttta actaaaagat aagtgaccgt gttgacatag ttttagcgag
    77341 aaattaattc tccataggag agcaatatca catcgcagaa ttacagtgag aacgtgcata
    77401 aatttagcgg gaaaagacat aagggaaagc caatttgtca gacaaattgt cgaatgcaca
    77461 gcagattaat ccataagatt agcctggaaa tccttgttgt ctttggtacc catgcgggat
    77521 gtcttctttt taaccagtca ataggccgca ttacctggcg ttgagttttt gaaatggtgt
    77581 aataaccgca actcaaagat gtggaaaatg cacgtcattc atttcgtcat taattatcac
    77641 tgtgctcatt aattaacaga acacgtataa tgagagccat ctcgcaaaaa tgaaaaaacg
    77701 ttttataaaa tcatcacttc atcatgaatt caaattcatt gattaatatc aacaagatac
    77761 aaaaagcact atcattaaaa ttcattgcag ttacattgat ttcatcaatg aaatgtaaaa
    77821 atatataaac ttgatgattt aagcattttc ttatacccgt tcagacgtta ttcttatttc
    77881 agatcatcgt cagaattgac tccacgatca catttcggac cggcagaaag gaattattct
    77941 gcaaacagta attatggtgt tttgatttat cttgcacctc tccacttctg gatataagga
    78001 tattaggtat ggcaaccgct ggaatgcttc tcaaactcaa ctctcaaatg aaccgcgagt
    78061 tttacgcatc caatctctac cttcacctga gtaactggtg ttctgaacag agtctgaacg
    78121 gcaccgccac tttccttcgc gcccaggcac agagtaatgt gacccaaatg atgcgcatgt
    78181 ttaactttat gaagagtgtc ggcgctaccc ccatcgttaa agccattgat gttcccggtg
    78241 aaaaactgaa ctctctggaa gaactgttcc aaaaaacgat ggaagaatac gagcaacgtt
    78301 ctagtacgtt ggcacagtta gccgatgaag cgaaagaact gaatgatgat tcaaccgtca
    78361 atttcctgcg cgatctggaa aaagaacagc agcatgatgg tctgttgctg caaaccattc
    78421 ttgatgaagt gcgcagtgcg aaacttgcgg gtatgtgccc tgtgcagacc gaccaacatg
    78481 ttctgaatgt cgtgtcacac cagctgcatt gatcatcatc ggcgctaatg cattgcgccg
    78541 atgaaggttt tgagaaaccg ctgcctcatc tgtttgaagc agcggttttt ttaatgggat
    78601 tcaccctgtg gggtaaactt gagttcaata agcgcgatgg ctttttggat tgcccgcatg
    78661 gtgaccgggt ctgcggcggc gggatggtta gtaaagtcga tattcttcag ctgactggac
    78721 attttttcac gaacttcaac gggcgcgatt acatcgagaa catccagaat ttgtttgata
    78781 accaactggc aagcaaccac atcagaaacc agttcctgat cggcattcag cggctgggac
    78841 atcgtaaact cctgatagca ttttgaaagc cgttatagta gcggcttcac atcttcagcg
    78901 atagtcacat ccaccgtcat caggacacaa aaaaacctgc cggagcaggt tttttattat
    78961 cggaacatat tgcctggcgg tacgtctttg aacgtcttgc aatagttatt gaacatactt
    79021 ttcaggattt tgcgcagttt catcgcggca ctccgaccat ttgttataca ggtgttattc
    79081 tctttgcgct tataatatga caaccatcac aaaaatcaat ctttatgtga tacaaatcac
    79141 ataaataccc ctttaatgtt ataaaaatga taatcaaaaa acagcccccc tatttctgac
    79201 acctacagat ggcaagaaat agcgcctgcc aggcgtcttt tccggccatt gtcgcagcgc
    79261 tgtaacgcgt aaaatagtgc tttctcttac tcttctggct ggaccatgag acttctgatt
    79321 ctgactcttt cattaataac gctggcgggg tgtacggtta ctcgccaggc ccatgtgagc
    79381 gaagttgacg cggcaacagg tattgtacgg ttggtttatg accaggcttt tttgcagcat
    79441 gcccataccg atcgttatgt cagtcgcggg atcgccgatc gcgcatgcca gcaggaaggc
    79501 tatacccacg cggtcccctt tggtcagcca gtaggcaact gtagcctttt tgcgggttcc
    79561 ttatgtttga atacggaatt cactttgtca tatcagtgcc atcattccgc cttccctgtc
    79621 tttctctaaa tattttaatc agcgagggga tcttcgctga ttaaagaaat agcggtaatg
    79681 cgttttattc ccattcgtat ttttaataat tgaagtttat at
//
LOCUS       DAZGSP010000005        78238 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZGSP010000005 DAZGSP010000000
VERSION     DAZGSP010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 78238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.005"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(177..389)
                     /gene="rpmE"
                     /locus_tag="ACHK74_000380"
     CDS             complement(177..389)
                     /gene="rpmE"
                     /locus_tag="ACHK74_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFP0219266.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            592..2790
                     /gene="priA"
                     /locus_tag="ACHK74_000381"
     CDS             592..2790
                     /gene="priA"
                     /locus_tag="ACHK74_000381"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFP0219267.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWQTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2946..3971
                     /gene="cytR"
                     /locus_tag="ACHK74_000382"
     CDS             2946..3971
                     /gene="cytR"
                     /locus_tag="ACHK74_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFP0219268.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4063..5022
                     /gene="ftsN"
                     /locus_tag="ACHK74_000383"
     CDS             4063..5022
                     /gene="ftsN"
                     /locus_tag="ACHK74_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFP0219269.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5115..5645
                     /gene="hslV"
                     /locus_tag="ACHK74_000384"
     CDS             5115..5645
                     /gene="hslV"
                     /locus_tag="ACHK74_000384"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFP0219270.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5655..6986
                     /gene="hslU"
                     /locus_tag="ACHK74_000385"
     CDS             5655..6986
                     /gene="hslU"
                     /locus_tag="ACHK74_000385"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFP0219271.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            7053..7979
                     /gene="menA"
                     /locus_tag="ACHK74_000386"
     CDS             7053..7979
                     /gene="menA"
                     /locus_tag="ACHK74_000386"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFP0219272.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8072..8557
                     /gene="rraA"
                     /locus_tag="ACHK74_000387"
     CDS             8072..8557
                     /gene="rraA"
                     /locus_tag="ACHK74_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFP0219273.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8666..9658)
                     /locus_tag="ACHK74_000388"
     CDS             complement(8666..9658)
                     /locus_tag="ACHK74_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0219274.1"
                     /translation="MSIKQLKDGRYKVDVRPQGTEGKRIRKIFTLKSKAQEFERYVLQ
                     NFHDKPWQAKPADQRRLSELIDAWWVLDGRNQPYGDTYRVRLEKINREMRDPRASEMT
                     RKFMLGYRSDKLQAGLMPSSVNRDLCALSAMFTSLIDAEVFHNENPVRGIRKLKVRNT
                     EMAFLSDDEIDRLLERLEGDARRVAVLCLSTGARWGEAAGLRGEHIVGNRVMFFNTKN
                     GKSRAVPVSDTVLSLIKTRKTGLLYQADYIRFRDILQEVKPDLPKGQATHVMRHTFAT
                     HFMMNGGNIVTLQRILGHATIQQTMTYAHFSPDFLQDAISFNPLADSVHKLSID"
     gene            complement(9728..10069)
                     /locus_tag="ACHK74_000389"
     CDS             complement(9728..10069)
                     /locus_tag="ACHK74_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0219275.1"
                     /translation="MVNMSRDYAKKLKAIRNAENLTQSQFSQVTGVSVGTIGNYESGY
                     KPARIEVVEKVIQVERFEKYTMWILHDKTFPAAGQVAPALSLDGSIQSEGDQVSIGIT
                     QKSPRSRRNAG"
     gene            10173..10697
                     /locus_tag="ACHK74_000390"
     CDS             10173..10697
                     /locus_tag="ACHK74_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0219276.1"
                     /translation="MRKSIADKELSDTQALQENDSITESVGAANDDEHQEEKKKRTYK
                     KGATLRLDGPVAGLCSLEKGAAYIGLTKAALRVALQRNQMPGHKTRSNPEDENSDGVW
                     WFNAREWDKLADELPECEPPEWHNWKSYWTYDRQKRKFSPANKEDCQTINGKRVYTGR
                     KSKLEQHQYSEGNQ"
     gene            10749..10943
                     /locus_tag="ACHK74_000391"
     CDS             10749..10943
                     /locus_tag="ACHK74_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219277.1"
                     /translation="MTDSTQTKDLYSDLRFIIIQQQSGRMDPTRASKAWKEALTDQGI
                     DVISEVMAEVIFRLTRDESL"
     gene            10969..11304
                     /locus_tag="ACHK74_000392"
     CDS             10969..11304
                     /locus_tag="ACHK74_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFP0219278.1"
                     /translation="MKQQRNARINNNTERHVARQHFRKNSATATTEIHTTPDGHPVKQ
                     TGKHTWAIGDTGIVIHKSFRSPVTGRFNFTLTRGDDYFGQDFTFFEALRTADRLISGL
                     RFQYPGSKH"
     gene            11323..11565
                     /locus_tag="ACHK74_000393"
     CDS             11323..11565
                     /locus_tag="ACHK74_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFP0219279.1"
                     /translation="MIEAYKILINNALQRYHFELANRGVTKDESLRNSLRGAIMGLYN
                     CALGSDDREALEELKSIMTELFEGEIVEPYFTGVAA"
     gene            11562..11687
                     /locus_tag="ACHK74_000394"
     CDS             11562..11687
                     /locus_tag="ACHK74_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219280.1"
                     /translation="MSIFLSWVILIISVLMAIGIMRIIHELKDIELFFTGDKRRK"
     gene            11902..12132
                     /locus_tag="ACHK74_000395"
     CDS             11902..12132
                     /locus_tag="ACHK74_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219281.1"
                     /translation="MRTAESPGRKITARPANHHAHISMAARKAERAHICELLRELTET
                     AKETHTCPQETCAAIERLIAILANPDELKGCA"
     gene            12132..12713
                     /locus_tag="ACHK74_000396"
     CDS             12132..12713
                     /locus_tag="ACHK74_000396"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP0219282.1"
                     /translation="MNHLMIDTETLGNGPDAAIFAIGAVFFDPFTGKLGKQFEKFIDP
                     VDSERNGGTVNAATAVWWAGQSVEARACLRNADGTELAAVTEFLAFISRNMHDESPGN
                     SLTIWCKGASFDFPILKSAITRTAGEKSIPWCYWDERCMRPLIAMAESTGWRMPGRSG
                     KETIAHTALGDAVYQAKVVSEIWQRFTTPFLNI"
     gene            12948..13928
                     /locus_tag="ACHK74_000397"
     CDS             12948..13928
                     /locus_tag="ACHK74_000397"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HFP0219283.1"
                     /translation="MSKELLSGTPLKWVGGKSRIMNTLKAHLPEADCLVEPFVGGASV
                     FMNTSYKRYILGDSNGALINFWRAARDNTDSLIDEAKTLFLNHNNRGDYLIIRETFNA
                     ENHAFLSSRTGISDRRELRLAAMFLYLNRHCFNGLYRVNGKGDFNVPFGGYRKPYFPE
                     REIRAFADKANSTQTLLIHADFKDTLSSASHLFGMGNTLCVYCDPPYLRFSGEGNFTA
                     YGKPFDEHEHVRLRAALDRLSQETGGMTRITISNSDTPETRRIYRGYRMNCIQAPRSV
                     GARTREPAPEVIATLRQCNACGHYGGGYCPDCGPVTGDATYSVMFTPAST"
     gene            13964..14200
                     /locus_tag="ACHK74_000398"
     CDS             13964..14200
                     /locus_tag="ACHK74_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219284.1"
                     /translation="MNSENNKELLRELRLIARAHEETAKVVAAAGRPELVITSAILLR
                     ITMEAIRVIQDQESEIAALKKQLNEKSAGQRGQQ"
     gene            14197..14415
                     /locus_tag="ACHK74_000399"
     CDS             14197..14415
                     /locus_tag="ACHK74_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219285.1"
                     /translation="MIQESTVIRFTANGRQYEVDESLIDPGMTRQGSRNPEMHHIRLI
                     NGSHFCATNVEEVRVLTATRNTGGKAWQ"
     gene            14406..14756
                     /locus_tag="ACHK74_000400"
     CDS             14406..14756
                     /locus_tag="ACHK74_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP0219286.1"
                     /translation="MAVIYIAGPMTGCKDYNRTAFCAEDLKLRCAGNIVLNPAVLPDG
                     LSQQQYMSICIPMLMCADAIYLLDGWEESAGARAEYAMALKLNMPVSFPENRAAGETV
                     AQLFGKSKAAQATE"
     gene            complement(14730..15254)
                     /locus_tag="ACHK74_000401"
     CDS             complement(14730..15254)
                     /locus_tag="ACHK74_000401"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219287.1"
                     /translation="MWRINITWQLVIVCIRGVFLMSDKLPSDYDILFDLWYSYWLERI
                     TCVFYRRVSAALRISAFLVGVAAFVLCDFVVLVLFSVVLFLLWQCMAASFERRAEKAE
                     WQTVSYQRLISQRHVISKECMIARLSFVEESDSPIMECMINLAWNKACEAVGSSSRNH
                     VSISGRLLSRLCGF"
     gene            15578..15799
                     /locus_tag="ACHK74_000402"
     CDS             15578..15799
                     /locus_tag="ACHK74_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFP0219288.1"
                     /translation="MANRKQQRARAERLHIRSEINRRLFRATRVAQIMHINMLHERTH
                     ALSNRYCAAVFSYLAEDLAELQSLINQHR"
     gene            complement(15889..16083)
                     /locus_tag="ACHK74_000403"
     CDS             complement(15889..16083)
                     /locus_tag="ACHK74_000403"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219289.1"
                     /translation="MLRINITWQLVIVCIVNGEGESPVCGDAAGSLFTGAAVIVVPVV
                     QAGSQQGIHRPHPVKEVLNI"
     gene            16407..16628
                     /locus_tag="ACHK74_000404"
     CDS             16407..16628
                     /locus_tag="ACHK74_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFP0219290.1"
                     /translation="MANRKQQRASAERLHIRSEINRRLFRATRVAQIMHINMLHERTH
                     ALSNRYCAAVFSYLAEDLAELQSLINQHR"
     gene            16697..17113
                     /locus_tag="ACHK74_000405"
     CDS             16697..17113
                     /locus_tag="ACHK74_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219291.1"
                     /translation="MKSVKGLSDIQDFFNWMWAVDPLLASRLNNWHDYYRRACKQAAR
                     SVTTYGDFSFTVDGRYRVDVKGDGDGLGLFHINGTDSTAISWSSSEMALILEMTTHSV
                     AVKGIYTAEGFIGEYQRLLVLYRAAFQVATAGECDA"
     gene            17110..19623
                     /locus_tag="ACHK74_000406"
     CDS             17110..19623
                     /locus_tag="ACHK74_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP0219292.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RIDARLDEETVAFLTTTDEGKRINSHLHHNEERRIRLEKLAKHKRENPPTVVSEAMAE
                     LRALPSFLGNVLIRDLARLNRKQQSARNEGVKNNNTHVADNFVRRGLRTRLKRIEQVN
                     ECFATTAFKATAARHRLDELLMLPQLNRDQIQRLATLTAAAFSTELERICDEVTERTG
                     KGDDNLFTWLLTYQQLARMALKLGVTPPYWPSLEIRRDRRTAPDPELVPGAVMRITCA
                     TWWNNQLRHLADLWREELLRAAGRVSRKASPYISHESLQEFREKRQRTRDFLKSWDIE
                     NEDGERLSLEDVYWSGLGNPRNRRNEMMACVRGMEQVAESRGDSAFFVTVTCPSRFHS
                     VNEDGSLNPKYNGATVRDASDYLVYDVFAAARKKLNKEGLSWYGVRTVEPHHDGTPHW
                     HMLVFTAPDNEERIIEIMRDAAIKSDRAELGNDISPRFKCEKIDPAKGTPASYIATYI
                     GKNLDASTFHDNDPKTGKPYADKESGKTMAETVENAIAWASLHRIRQFQFFGIPPRQV
                     WRELRRLAGQMERNPASPKRLDHDDIDAILAAADVGCFATYINRQGGVLIPRNLYLIR
                     TAYETADEANDYGEFPQRIYGICAPSLGERYTICTHPDEWKLVRKEAKADNSTDEDFD
                     VGGGFAAPWTRGNNCPHDEKTDTGEGKTPPPETRKEQKLTIPEGDLGEWLRSLTPTER
                     KQLTRQLKDAPSDTKTVKTGGSTTPDDVIHVRTALPLTDVTTTVIKSALSTTGEELHS
                     GAALSVHRGARVRLPDGRIVHWDEKSRSLVAADQPEVNP"
     gene            19620..20024
                     /locus_tag="ACHK74_000407"
     CDS             19620..20024
                     /locus_tag="ACHK74_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219293.1"
                     /translation="MTRFHCVLQRHDFSTIMNARIPALTLSGVSVRPQPGDELEITIA
                     SPDRSTDTVMTDILTARINTDRSAFIVAVRPRRAISEPEPLTPVGKIMQAYHEKQTRK
                     LNEELARTLAVVNRKAAIVSYVHSAASRQSRR"
     gene            20034..20243
                     /locus_tag="ACHK74_000408"
     CDS             20034..20243
                     /locus_tag="ACHK74_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219294.1"
                     /translation="MNMTRNSKPKEHTMNDIRRISDKELTKMIESAQQLLSRRNPVVE
                     ETWWENLLAALTELRERRNNEGAAK"
     gene            20240..20578
                     /locus_tag="ACHK74_000409"
     CDS             20240..20578
                     /locus_tag="ACHK74_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP0219295.1"
                     /translation="MKTPRMTGKVIILRYLETHARFSAPELVEKLGVKRTIINQAAGK
                     MVREGLLVVDGYTDKITHYRKPTEKERLELERRAEQQQKPSVIQDCKRSEIMKRILFI
                     YGAGEELPVI"
     gene            20611..20928
                     /locus_tag="ACHK74_000410"
     CDS             20611..20928
                     /locus_tag="ACHK74_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0219296.1"
                     /translation="MPTQVATEGRLAADPNTRRTTKGTNVTTAQLAVSLPCHGAEQPT
                     TMCLYLISFGKQHEELAKHRKGDLILVHGNLQVNRYQDKYGQQKEGWQLLTQSIISSR
                     TGR"
     gene            complement(20978..21514)
                     /locus_tag="ACHK74_000411"
     CDS             complement(20978..21514)
                     /locus_tag="ACHK74_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219297.1"
                     /translation="MKNYSYPYLTFVTLSVLGGIFLFIWWRNDVYRITYLYSFTTNCL
                     VSVSVVLSVFVSLCLLKKNLVDRSKFTGVTSYLKFFSGMCLLTWMILFTLLVTLIYFL
                     PGEDSFYSAPYEYSRGSSKSCSGAFVDDPDLQKTIFICYPYGDYQDGNVIYVKKRVNA
                     LGAVVTYAYATSGRFRFD"
     gene            22060..22563
                     /locus_tag="ACHK74_000412"
     CDS             22060..22563
                     /locus_tag="ACHK74_000412"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219298.1"
                     /translation="MSHFRDLYLANKKSRDARREVTGILRNQAEKLVAFYEQWLELPS
                     ETWEDKDGEHHRYVDTGFPCRNDRDFRPFPVNMIATDTDNIFRMAVRTWVDSHDEGES
                     VNIVLAMELHSVGKDGVSLRVRVEDDTSIRVVINGDGDREWLPIVNSMKMHIISKLQR
                     RTPSAFR"
     gene            complement(22629..23672)
                     /locus_tag="ACHK74_000413"
     CDS             complement(22629..23672)
                     /locus_tag="ACHK74_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0219299.1"
                     /translation="MSKRKKYTGKRGQDSLHDALKSTPALSAFTFDGPYEVSRMDLLD
                     NMSCADNGRYFDTPMDWNAIARASRRASWHQSALYFKRNALNGCFVPHPLLSRQAFSA
                     FALDWFVFGNAYLEVRRNRLGEPLLLRPALAKYTRRGSDLDTYWYLNDDGTEFSFRKG
                     TVCHVLNPDINQEIYGMPEYIGGLLSVSLSNSADTFRKLYYDNGSHAGCIIYVGTSQA
                     NAESVEAIKKTLTDSRGRGAFKNLFLHAPGGGKDGVQILPFQQITAKDEFLNIKGSAR
                     DDILAAHRVPPQLMGAMPDGNAAFGDVEKAARVFFINELQPVMEAMKHVNEWLGVEVM
                     RFNPYALLLDNAS"
     gene            complement(23678..25411)
                     /locus_tag="ACHK74_000414"
     CDS             complement(23678..25411)
                     /locus_tag="ACHK74_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFP0219300.1"
                     /translation="MIQDVFIRMRAKQLYWQGYTPADIAQLMGINQNTIYSWKKRDEW
                     DATPPIQRVTQSIDARLIQLTAKPDKSGGDFKEIDLLTRQLKKLNDGQGEQAVSGKKP
                     RRRKLKNHFTEEQIVALREKILPSLAWHQREWYDNRHHRNRMILKSRQIGATWYFARE
                     ALLDALRDDVSAGYQRNQIFLSASRRQAYQFKHFIQQAALEVDVELKGGDKIVLSNGA
                     EIHFLGTSAATAQSYTGHLKFDEFFWVSNFVQLRKVAGAMATLKGLTRTYFSTPSGET
                     HEAYQFWTGDRWNSERPRSKRTEFDVSHSNLKGGVLFPDKTWRQIVTIEDAVANGWEY
                     TSIDDIRDENTESEFRNLYMCEFVRDGEAAFSLNALIGCGVDGYDEWPDWKPFAARPM
                     GVREVWLGYDANGSSGKGDCGALSVCVPPRVTGGRFRTVETVRVQGMEFEQQAALIEE
                     YTQKYNVKHIGIDVTGGNGEGVYQIVKKFFPMAVPYNFNIASKRALVMKMLQVVRAGR
                     WEYDRSERDLIQAFASVRKIQTPGGYITYDTDRSRGVSHGDLAWATMLAVINEPIGAE
                     REDNRGFAMEF"
     gene            25567..26403
                     /locus_tag="ACHK74_000415"
     CDS             25567..26403
                     /locus_tag="ACHK74_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFP0219301.1"
                     /translation="MGSKNTPERKKFRVAVSGSTIDGREISGEMLRQAAENYDPSVYA
                     SRVNIEHVLSMWPSSELCAVGDLIALSTDEITEGKLAGRTALYAEIEPTDRMKNMIAD
                     GKKVYSSIEMLPELDAVKGPYIVGLAMTDTPASLGTERLEFAAQQRASIMQFSKCTTE
                     PPMFTVAMEAEMLTTTEQRTEESRQWFSRVMALISKTRTADGEQFSQLRDVVENIASD
                     HADLIDRFSTLEQKHALDKTALDELSLELAALKAQLRNQDADPQQRFTSAGGTDQTLA
                     EF"
     gene            26421..27521
                     /locus_tag="ACHK74_000416"
     CDS             26421..27521
                     /locus_tag="ACHK74_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFP0219302.1"
                     /translation="MARLSLSREGRDSLERYMIQQASLAGITTERLGKTFSVDPSIQQ
                     KMENAIKESAGLLQEINFIGVDDQEGEKVLIDTSGPIASTNATSDGVKRRNPASVADL
                     DARRYRCEQVNYDTFISYAQIDAWSSQKNFQQLLSAQITRQVALDRIMIGFNGESHAL
                     ISDRATNPLLQDVNPGWLKHIRDKAATRVVKGMTLTRRDDENKLVAKGDYGNPDAMVH
                     DIRSSVLDEWHKDAPDLVVLMGRDLFNTLRLPMINAISTTNPNTELVAGQLIVASRTV
                     GGLQTYLAPYFPKDAMLITSLKNLSIYFQKGSLRRFIREEPEYNRIATYQSMNDCYVV
                     EDYGKCALIEGITFAGEDPDNNAESGQPQPEA"
     gene            27562..28359
                     /gene="gpM"
                     /locus_tag="ACHK74_000417"
     CDS             27562..28359
                     /gene="gpM"
                     /locus_tag="ACHK74_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFP0219303.1"
                     /translation="MLTPAQRYVQQAVARNRVTMNARETQRERTAHEQVLHQLRLAQM
                     QLKGIQSNVARAELKKHLLPDFEGWIEGTLAGDSGRQDEVITTMMVWAIDCRDYPLAL
                     RIGEYVIRHGLALPDNFGRDAATVLAEEITDAVLTQATTDSEADLSVYTAMLDELNHQ
                     VSEQDMPDQVRSKLHKARAFSRRASSSPDDLHLSLNLLREAMRINPASGVKREIATLE
                     RAVRKLADGNQVVPASTTGRKTTTGGKKSSAKKKSAASQKKAARKTN"
     gene            28464..28958
                     /locus_tag="ACHK74_000418"
     CDS             28464..28958
                     /locus_tag="ACHK74_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFP0219304.1"
                     /translation="MSLIARPRVNSADTDTTDTDDGDAMVSAGDFWPAISLHEIRLAS
                     RIPGRTTTTRLLHAATEAVAHVIAELELWKRERMAEGAASLADVPATQVNGESVHVHR
                     YRRAVFALTRAFVLERTRDVDTTDKGDRRADALDMQVEDLWRDARWAISDIRGVTRIY
                     AELV"
     gene            28958..29158
                     /locus_tag="ACHK74_000419"
     CDS             28958..29158
                     /locus_tag="ACHK74_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFP0219305.1"
                     /translation="MKVMALDGDTVDLLCQRHYGTTQGVTEAVLAANPGISAVMFLHA
                     GQEVELPELQGQTRREMIQLWG"
     gene            29161..29445
                     /locus_tag="ACHK74_000420"
     CDS             29161..29445
                     /locus_tag="ACHK74_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0219306.1"
                     /translation="MFQRLCDHITFWASVTTAGIGVMTLSEKIAVAGFVLGTLSLLRA
                     WLHRRRMEIAQARRNELIEKILMQSEGRELNDAERRAMMLLKQDEHDGRT"
     gene            29432..29974
                     /locus_tag="ACHK74_000421"
     CDS             29432..29974
                     /locus_tag="ACHK74_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0219307.1"
                     /translation="MAGPKRYAVAAIVALGISLAPGELRTSREAQIKIATWEECRARP
                     YRDLVGVGTVGCGSTGNVENRLYTQEEVAGRWINDMRRAENCITQNFRGQQMPQSAFE
                     AMTDAAFNLGCRNLMWFKNKNGTPQRTTIWKHAQTRQWRLMCYRLTDFVNSGGTRTQG
                     LVNRRNDFKNWCLKDAGAQP"
     gene            29971..30396
                     /locus_tag="ACHK74_000422"
     CDS             29971..30396
                     /locus_tag="ACHK74_000422"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFP0219308.1"
                     /translation="MKLNIILCALLLTISLQAMVVIRAYRSGQESTRYEALEKAVKES
                     NTTVRELLKATAETQSALAAIRREDQERAARGEERRETMRKNLQADECARTSPPADVA
                     DSLQQHASAAENRRHLRERAGKSDQTDAGTGAAATHDMG"
     gene            30383..30514
                     /locus_tag="ACHK74_000423"
     CDS             30383..30514
                     /locus_tag="ACHK74_000423"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219309.1"
                     /translation="MIWADVADWGDRLVDALDSCNADKAAIAGLERQRTARMNTHEK"
     gene            30525..32570
                     /locus_tag="ACHK74_000424"
     CDS             30525..32570
                     /locus_tag="ACHK74_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP0219310.1"
                     /translation="MVPKHYQLVTAMTNKELAEKLAPMLREGWGLLGGPVSASEPGAP
                     FYLMQALVTDRDLPSAIPVPEGEEKPVPGTTDGYNTGHTTEPEFYYVVPLAGQSNGMA
                     YGEGLPLPDTFDSPDPRIKQLARRSTVTPGGAACTYNDIIPADHCLHDVQDMSSLNHP
                     NADLSKGQYGCVGQGLHIAKKLLAFIPKNAGILLVPCCRGGSAFTSGAEGAFSESSGA
                     TAESSLWGVGKPLYNDLLTRTKAALDKNPKNVLLSVVWMLGEFDLKSATYAQYPQLFL
                     NMVNQFRADLSAYAAQCTGGNASLVPWICGDTTYIWKQQYTSQYPVVYGAYQNKEAQK
                     IYFVPLMTDEKGANTPTNLPAEDPDLAEAGYYGAASRSNGNWTSSDRATHFSSWARRG
                     IISDRLATAILMHAGKTVAFLKGAPAPFSVLAVPPATAGSDSTTTESPQPSTPAPQPG
                     AGTQSSPARTTAVAYRADAQEGKLKEQGWTGADGKASVVNDSAAESGKAMRVEKVQGQ
                     QTAWKIAHDVPASKAAELLQKGGEIAFRFKIPEGVQLAENRFAMALHWPVSQLPENVV
                     LEGSAGNDMLAAFFIQSDSANLNLMQHKQQNAKLGTFGAFNHNWHNVVFRFAGNNSIS
                     VTPVINGQTQAAFNLAKCPAAGLDNDKLLITDITSNQDTYPVLIEYVTVDINNSNAA"
     gene            32717..32890
                     /locus_tag="ACHK74_000425"
     CDS             32717..32890
                     /locus_tag="ACHK74_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0219311.1"
                     /translation="MLFIHQIMLYFCTAVCALYLISGGYSVIRNYFQRKIDERAAEKI
                     SQKSAQQNNQNMP"
     gene            32906..33373
                     /locus_tag="ACHK74_000426"
     CDS             32906..33373
                     /locus_tag="ACHK74_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0219312.1"
                     /translation="MLKINTLRDAMTDACPWCRANPEKFTVYVNNGGIETTGESPSYL
                     YRYTLGIFVMDFTQDLDELMVPLMRWLYRNQPDLLLNPEKNRDIKFSVAINDDDSTDI
                     LLEIPVWERVIIRRNDDGTVHAEHIGEPPVSRALEAWRVTLEDVTWGQNDGSG"
     gene            33360..34001
                     /locus_tag="ACHK74_000427"
     CDS             33360..34001
                     /locus_tag="ACHK74_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFP0219313.1"
                     /translation="MAPDDLHRLDGLFQEILSGLSAAGINKTARAVGQAVRAAQQKRI
                     RSQQSPDGNRWPARKKRVFRVQAGVVFIWNGTQVRRLKNWRTTTGRYGPMITGYDTER
                     RGIRSFHKADIEEYLEIDTRRRTQSAIKRPPMFEQLRTNRFLKVKTDPGGVSVGFEGR
                     AARIARVHQFGEVSLVSAGNAVRYPQRELLGFSEADRQKVTEIIINNLWRNTR"
     gene            33998..34579
                     /locus_tag="ACHK74_000428"
     CDS             33998..34579
                     /locus_tag="ACHK74_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009486481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFP0219314.1"
                     /translation="MNAEIMRLLENMVRTGVVSEVDLKNWRIRATSGGLTTDWLRWNC
                     TRAGAFKIWLPPSPGEQVLLLCPGGSPEMAVVAGSLYSEEHPAPGTKQNEVILLAPDG
                     AEIRYDAEKGALTVKGIKTAEISAETRITLDTPAVECTKHLKVRTFELTDGGTLKGDI
                     THSNGNLLSNGVTVHTHVHNGVQSGGSMTGGPK"
     gene            34576..34941
                     /locus_tag="ACHK74_000429"
     CDS             34576..34941
                     /locus_tag="ACHK74_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFP0219315.1"
                     /translation="MTPVCTGMNPHGSGCISDTEHLRHSVNNILTTPRGSRIMRREYG
                     SLLPELLDAPQNDVTRLQCMSAVVMALAQWEPRIAINRVDIAWLPDGRALLAISGVLT
                     RTMQPVQMSIPLRGSNADN"
     gene            34928..35824
                     /locus_tag="ACHK74_000430"
     CDS             34928..35824
                     /locus_tag="ACHK74_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFP0219316.1"
                     /translation="MPTIDLSQLPRPQIIESLDYESILKDVKAFMIDAFPENMRSAVS
                     AAIALESEPLNIIAQAIAFREMLLRQRINDAAAGCMLSHAVSTDLDNLAAIVNAKRLE
                     LKPATDTEAAIMESDSAFRLRAQSGFEGFSVAGPSAAYEYFARSASGLVADARATSPS
                     PATVIVSILSTEGDGTASPELIATVSRALNDETVRPLADRLTVQSATIVSYDIEAVLY
                     FYPGPESEPILAAARNALDTWLRTQNAIGRDVARSAIMATLHVPGVQRVELKKPAQNV
                     VISNTQSARCTSRNISAGGTDE"
     gene            35817..36440
                     /locus_tag="ACHK74_000431"
     CDS             35817..36440
                     /locus_tag="ACHK74_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFP0219317.1"
                     /translation="MNNRLLPPSSPSFLHHSEQGAQRLSELPIHINTLWNPDTCPAHL
                     LPWLAWALSVDRWDETWSEEARRQIIRDSWHIHRHKGTISAMRRAVEPFGAILSITEW
                     WENNDPPGSFRLDIDISERGLTEETRQEMERMITTVRPVSRHLAAMKMTYVARGVSRI
                     TGATLTGETIVVYPAAELTSPLSVSGDIHPGGVTYEGITVTIRPDKE"
     gene            36443..37042
                     /locus_tag="ACHK74_000432"
                     /pseudo
     CDS             36443..37042
                     /locus_tag="ACHK74_000432"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            37349..37780
                     /locus_tag="ACHK74_000433"
                     /pseudo
     CDS             37349..37780
                     /locus_tag="ACHK74_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764700.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            37792..38379
                     /locus_tag="ACHK74_000434"
     CDS             37792..38379
                     /locus_tag="ACHK74_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFP0219318.1"
                     /translation="MHTIKSARNAAYNRDGTIDCEIQFEGEESWLPYTATEYDNSPHG
                     KKLWGELKAGNHGPVSEYQENDTDRENARRLKNDEINTWRNAMEAASYTFEHNGRKWD
                     YGKSTQARLEPSVAAAKAGILPLDFFWTDAQNNDVPVSADELIALSDAAAKALFAKGM
                     EIHIRQRNMKKEIAALNDTAAILDYKVGWGKEAEA"
     gene            complement(38390..38881)
                     /locus_tag="ACHK74_000435"
     CDS             complement(38390..38881)
                     /locus_tag="ACHK74_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0219319.1"
                     /translation="MMMIYGMFVFQLSTLPHQQVEQSKRWRHVSNERVNRSSSWQYIG
                     SGEDHITMSGVLCPEITGGEVSLTALNTLAYTGLPWPLIDGVGQIYGMYVITEIRTTR
                     QELDRYGMARKIEFTVSFQRCNEDLRERLQSGSVGDLMAGLKNSASSALSSASSALSG
                     LML"
     gene            complement(38892..41684)
                     /locus_tag="ACHK74_000436"
     CDS             complement(38892..41684)
                     /locus_tag="ACHK74_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0219320.1"
                     /translation="MMNNLHIRVALAAADTLTQPLRRARETVGALSDSLRETQDSIRS
                     LERQSEAFAAAQTKFDEATRKIAAVQQQLNRLQQAEREGTVLSERQREMMASLTTRLE
                     RLNRVRDVHQRRLEDSRRELERHGIAAEGSSQTIQAAIRRTAEYNEALARQRQALAQV
                     TRAQQRYEQAQERLGRIRNAGAAALAAGAAGLYAGGRAVAPVMDLQRHGAVIAAQAGE
                     GASDAVGYTGVINHMVHTGITPDAQLAAEAVGAVRSTLGGLGAVSENELTRITRKMLD
                     MQAVMGGDIPQHIQAAAIMIKNGLARSSDEAVDLMVAGMQRMGPVMRDELPEILHEYS
                     THFRNMGLTGSETMTLLADMAQQGKFALDKTGDAIKEFSIRGSDMSKASAEAYALIGL
                     DAARMSSAVASGGTAARAALQKTASGLLQIVDPAERANAAIALFGTPVEDLSVDQIPA
                     FLGALARVSDRMADVSGVADRMGQTLRDNLSGDVARLTGTFSALRSGIIGEITDDLRG
                     LVRMATAWGERLQAWVNAHPELVRMIALTTGAVLALTTAMGALSVAAWMVVAPLLKLR
                     LGLALLRAIPAGLSVAGTLFGGLTGVISAVGAALGALTWPVVVAVGALVGGALLVWRY
                     WDRVRAFVSGFIEGVQSALAPFMATASRFAPLLDLVSGALSRILGWFKSLLTPVSSTR
                     DLLEQCTSAGQMFGSTLAGALATVFSPLKALIDGLAWVLEKLGVLPGEAERVKRKLDE
                     ARKTPVAWEWDPEQKKMVQKAWHWSPPGGASVPSGASGTQPPPVALPAGIAPDGNRNV
                     LRHLKGIEGNTGGLLAENRKRIGPGDIVFKHLPRALAVRGQWHEPVVAPSSGGMRALP
                     QEEIQVQQWPTARPAPRMHQQETFASAFQGEIHVHLHGVSSHNPRELARMVGDAVRAE
                     MNRMARAGTGSFRDRD"
     gene            complement(41674..41811)
                     /locus_tag="ACHK74_000437"
     CDS             complement(41674..41811)
                     /locus_tag="ACHK74_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFP0219321.1"
                     /translation="MEDPVADIAVIFNWPPAEIFAMTPAEVVAWRERAAARSGSHNHD
                     E"
     gene            complement(41832..42221)
                     /locus_tag="ACHK74_000438"
     CDS             complement(41832..42221)
                     /locus_tag="ACHK74_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219322.1"
                     /translation="MDKETEMATGTAVSNGEASAVAVAATAAPKNVVKLTVPVVRGDV
                     SYSEIKIGPLANQAGSLRGLSLLALYNMNCDAMVTWLARVTEPKLKEAEMYAMNTADF
                     TALSSAAVNFFAPSGSNAPAPENTAEP"
     gene            complement(42269..42778)
                     /locus_tag="ACHK74_000439"
     CDS             complement(42269..42778)
                     /locus_tag="ACHK74_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFP0219323.1"
                     /translation="MAVPNKLRVFTVYIDGENKLGRVTSFTPPKLTRKTESFRGAGMP
                     GSVSVDFGLDDGALDLSFAVGGADAHLFSRYAGSLDEVRLRFSGEYYSDADIRYVDIE
                     VRGRITEIDMGEAKQGEDTEHSYSMKNTWYRLSVDDLEKIEFDVLNFIWRIDGKDVLP
                     DRTRSMLGF"
     gene            complement(42778..43965)
                     /locus_tag="ACHK74_000440"
     CDS             complement(42778..43965)
                     /locus_tag="ACHK74_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFP0219324.1"
                     /translation="MAETRFHGVRVRENADLVTAINDIDSSVIGIVAVADDADAKTFP
                     LDKPVLLTRVMDVLGKAGKTGTLYKSLKAIADQVSTRVVVVRVAAAKEGDGQPTQTQL
                     VIGEVKNDGTYTGMQALLVAEQNEAVGVRPRILAVPSLDTKEVVAALVTVAKKLRAFV
                     YAGCPGCNTVQEAVTWRADLAHREVMLIWPDFIAYNPNSGQNEAFPASAYACGLRAKI
                     DHEQGWHKSLSNVAVNNVLGISVPVFWSLQAEDSDAETLNDKEITTLIKRNGFRFWGN
                     RTLDTTTYTFETYTRTAQSLADTIAESQFETVDKPLTPSNVKDVLSDIRAKLSALVTA
                     GKLIGAECWYDVVDNGTTDLRQGIVRIRYRYTPVPPLEDLELHQTFTDEFFDGAFASL
                     GGE"
     gene            44120..45244
                     /locus_tag="ACHK74_000441"
     CDS             44120..45244
                     /locus_tag="ACHK74_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFP0219325.1"
                     /translation="MVLLNGNTVYSPLKELVKMPDFSVSLDREQDKNTLQELNNRVIS
                     LSMTDNRGFEADRVTLVLDDADGQLALPGRGTHIRVMLGWKGEPLVDKGTYIVDEVAH
                     EGPPDRLTLSANSADFREEFNVKREVSWHDVTVERVVSAIAHRYGLKAQISDMLMDIE
                     IDHADQTEESDMSFLTRMADMLGAITTVKNGCLLFILPGGGVTADGRPLPSFALARSD
                     GDSHQFRIADRQAYTGVKAYWLDLNHGKKKAVNIKKRKTHAEKSSSREGSYMEGAEGN
                     VFVLRKTYPNEIAAQRAAAARWQQLQRGAAEFSITLARGRAELYPEMHGTVTGFKTEI
                     DNQDWLIAKVEHKIDGSGFTTRLELEARIPEWIAEKESNG"
     gene            45289..45591
                     /locus_tag="ACHK74_000442"
     CDS             45289..45591
                     /locus_tag="ACHK74_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFP0219326.1"
                     /translation="MFHCPFCGASARIRTSKAANDTGTIRYKYYQCNNLECSATFSTM
                     ESVHRIIGQPQLLFHDKDIPRETMRKSHQGRSQLTLQLEDCGISSKPVHYARVPFG"
     gene            45726..45866
                     /locus_tag="ACHK74_000443"
     CDS             45726..45866
                     /locus_tag="ACHK74_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0219327.1"
                     /translation="MPQKTIIVSILCLTMLLAIWMIHRSPCEFRLNIMWSEFAAFLQC
                     KQ"
     gene            46198..46338
                     /locus_tag="ACHK74_000444"
     CDS             46198..46338
                     /locus_tag="ACHK74_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0219328.1"
                     /translation="MSQKSLMAITFCVTAILIIWMLHGSLCEIRMNIWGAEFAAFLQC
                     KQ"
     gene            complement(46616..46861)
                     /gene="zapB"
                     /locus_tag="ACHK74_000445"
     CDS             complement(46616..46861)
                     /gene="zapB"
                     /locus_tag="ACHK74_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFP0219329.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            47286..48131
                     /gene="glpF"
                     /locus_tag="ACHK74_000446"
     CDS             47286..48131
                     /gene="glpF"
                     /locus_tag="ACHK74_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFP0219330.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            48154..49662
                     /gene="glpK"
                     /locus_tag="ACHK74_000447"
     CDS             48154..49662
                     /gene="glpK"
                     /locus_tag="ACHK74_000447"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFP0219331.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            49798..50808
                     /gene="glpX"
                     /locus_tag="ACHK74_000448"
     CDS             49798..50808
                     /gene="glpX"
                     /locus_tag="ACHK74_000448"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFP0219332.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            50905..51651
                     /gene="fpr"
                     /locus_tag="ACHK74_000449"
     CDS             50905..51651
                     /gene="fpr"
                     /locus_tag="ACHK74_000449"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFP0219333.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(51656..52084)
                     /gene="uspD"
                     /locus_tag="ACHK74_000450"
     CDS             complement(51656..52084)
                     /gene="uspD"
                     /locus_tag="ACHK74_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFP0219334.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(52111..52410)
                     /gene="yiiS"
                     /locus_tag="ACHK74_000451"
     CDS             complement(52111..52410)
                     /gene="yiiS"
                     /locus_tag="ACHK74_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFP0219335.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(52622..53062)
                     /gene="yiiR"
                     /locus_tag="ACHK74_000452"
     CDS             complement(52622..53062)
                     /gene="yiiR"
                     /locus_tag="ACHK74_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP0219336.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFIGTQGENKYGKDTQDVKYKADNKSSN"
     gene            53163..53762
                     /gene="yiiQ"
                     /locus_tag="ACHK74_000453"
     CDS             53163..53762
                     /gene="yiiQ"
                     /locus_tag="ACHK74_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFP0219337.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            53870..54637
                     /gene="tpiA"
                     /locus_tag="ACHK74_000454"
     CDS             53870..54637
                     /gene="tpiA"
                     /locus_tag="ACHK74_000454"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFP0219338.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(54692..55447)
                     /gene="cdh"
                     /locus_tag="ACHK74_000455"
     CDS             complement(54692..55447)
                     /gene="cdh"
                     /locus_tag="ACHK74_000455"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFP0219339.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(55554..56543)
                     /gene="sbp"
                     /locus_tag="ACHK74_000456"
     CDS             complement(55554..56543)
                     /gene="sbp"
                     /locus_tag="ACHK74_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFP0219340.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(56863..57825)
                     /gene="pfkA"
                     /locus_tag="ACHK74_000457"
     CDS             complement(56863..57825)
                     /gene="pfkA"
                     /locus_tag="ACHK74_000457"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFP0219341.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(58006..58908)
                     /gene="fieF"
                     /locus_tag="ACHK74_000458"
     CDS             complement(58006..58908)
                     /gene="fieF"
                     /locus_tag="ACHK74_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFP0219342.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(59057..59557)
                     /gene="cpxP"
                     /locus_tag="ACHK74_000459"
     CDS             complement(59057..59557)
                     /gene="cpxP"
                     /locus_tag="ACHK74_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFP0219343.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            59707..60405
                     /gene="cpxR"
                     /locus_tag="ACHK74_000460"
     CDS             59707..60405
                     /gene="cpxR"
                     /locus_tag="ACHK74_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFP0219344.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            60402..61775
                     /gene="cpxA"
                     /locus_tag="ACHK74_000461"
     CDS             60402..61775
                     /gene="cpxA"
                     /locus_tag="ACHK74_000461"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFP0219345.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(61881..62555)
                     /gene="yiiM"
                     /locus_tag="ACHK74_000462"
     CDS             complement(61881..62555)
                     /gene="yiiM"
                     /locus_tag="ACHK74_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFP0219346.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(62704..63687)
                     /gene="kdgT"
                     /locus_tag="ACHK74_000463"
     CDS             complement(62704..63687)
                     /gene="kdgT"
                     /locus_tag="ACHK74_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFP0219347.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(63947..64567)
                     /gene="sodA"
                     /locus_tag="ACHK74_000464"
     CDS             complement(63947..64567)
                     /gene="sodA"
                     /locus_tag="ACHK74_000464"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFP0219348.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEASARFAAKK"
     gene            64852..65886
                     /gene="rhaT"
                     /locus_tag="ACHK74_000465"
     CDS             64852..65886
                     /gene="rhaT"
                     /locus_tag="ACHK74_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFP0219349.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRHPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(65883..66731)
                     /gene="rhaR"
                     /locus_tag="ACHK74_000466"
     CDS             complement(65883..66731)
                     /gene="rhaR"
                     /locus_tag="ACHK74_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFP0219350.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(66805..67641)
                     /gene="rhaS"
                     /locus_tag="ACHK74_000467"
     CDS             complement(66805..67641)
                     /gene="rhaS"
                     /locus_tag="ACHK74_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFP0219351.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVAEQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            67929..69398
                     /gene="rhaB"
                     /locus_tag="ACHK74_000468"
     CDS             67929..69398
                     /gene="rhaB"
                     /locus_tag="ACHK74_000468"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFP0219352.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLKERKINDLPALIVATQSLPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVARIHSTRKTKELCA"
     gene            69395..70654
                     /gene="rhaA"
                     /locus_tag="ACHK74_000469"
     CDS             69395..70654
                     /gene="rhaA"
                     /locus_tag="ACHK74_000469"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFP0219353.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLELD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFNRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            70896..71720
                     /gene="rhaD"
                     /locus_tag="ACHK74_000470"
     CDS             70896..71720
                     /gene="rhaD"
                     /locus_tag="ACHK74_000470"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFP0219354.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            71730..72044
                     /gene="rhaM"
                     /locus_tag="ACHK74_000471"
     CDS             71730..72044
                     /gene="rhaM"
                     /locus_tag="ACHK74_000471"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFP0219355.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            72345..72791
                     /gene="frvA"
                     /locus_tag="ACHK74_000472"
     CDS             72345..72791
                     /gene="frvA"
                     /locus_tag="ACHK74_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFP0219356.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
     gene            72802..74253
                     /gene="frvB"
                     /locus_tag="ACHK74_000473"
     CDS             72802..74253
                     /gene="frvB"
                     /locus_tag="ACHK74_000473"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFP0219357.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSHLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            74243..75313
                     /gene="frvX"
                     /locus_tag="ACHK74_000474"
     CDS             74243..75313
                     /gene="frvX"
                     /locus_tag="ACHK74_000474"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP0219358.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRNILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEVEKRGVEIGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAAFKNCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            75313..77061
                     /gene="frvR"
                     /locus_tag="ACHK74_000475"
     CDS             75313..77061
                     /gene="frvR"
                     /locus_tag="ACHK74_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFP0219359.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERACCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEII
                     AGLTGYEAFMELLHKG"
     gene            complement(77111..78151)
                     /gene="yiiG"
                     /locus_tag="ACHK74_000476"
     CDS             complement(77111..78151)
                     /gene="yiiG"
                     /locus_tag="ACHK74_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFP0219360.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFK
                     QGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEAVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALRE
                     YNEMVDDYNSLR"
BASE COUNT        19729 a        21020 c        20281 g        17208 t
ORIGIN      
        1 gccggttttc cgctcatcgt aaaatccttt tgaaataaat aagaaccaga agcctagcgc
       61 cagtcaacaa caggaaatga taatgagtta acaccccgga atttttcaga atttataaaa
      121 ggcaaattac aggcacaaaa aaagcgccgt gcggcgcttt tttcggaaat ccggtcttat
      181 ttgctgcccg ggatgttgaa acgcttgttg aagcggtcaa cacggccacc ggtagcaaca
      241 tcacgctgtt tgccagtgaa gaacgggtgg cacttgctgc acacgtcgag gttcaggtca
      301 tgaccaacgg tggagcggat tttcattacg ttaccgcaag agcagctagc agtaatttct
      361 tcgtatttcg ggtgaatatc ttttttcatg ggaaaacctc ggtttaaggc cgcgtcgctc
      421 ttccagccct aacgccagac accacgcgat gttaaaagta tagcttcaat acgatcattt
      481 cgtacgaagg cgcgaaatca tacagaaatt aaccagcgta tgcaaactga tccgcactct
      541 tctacggcaa tgtgtatact aacccaccga atttcaagtc aggatgatgc tatgcccgtt
      601 gcccacgttg ccttgcccgt tccgcttcct cgtacctttg actatctgct gccagaaggc
      661 atgacggtta aagccgggtg tcgcgtgcgc gtgccgtttg gcaaacagca ggagcgcatc
      721 ggggttgtgg tgtcagttag cgatgtcagt gagctgccgc ttaacgagct gaaagcggta
      781 gtggaagtgc tggacgttga gccggtattt actcactccg tctggcggtt actgctctgg
      841 gcggcagatt actatcatca tccgattggc gatgtgctgt ttcatgcctt gccgatttta
      901 ctgcgccagg ggcggcctgc ggcgaacgcg ccgatgtggt actggtttgc cactgaacaa
      961 ggccaggcgg tggatctgaa cagcctgaaa cgctccccca agcaacaaca ggcgctggcg
     1021 gcgttacggc aaggcaaaat ctggcgcgac caggtcgcca cgctcgaatt taatgatgcc
     1081 gcgttgcagg cgctacgcaa aaaaggtctg tgtgatttag caagtgaaac accagagttt
     1141 agcgactggc aaacgaacta tgccgtttct ggtgagcggt tgcgattgaa taccgaacag
     1201 gccaccgccg ttggcgcaat tcatagcgcg gcagatactt tttctgcctg gctgctggcg
     1261 ggcgttaccg gttccggtaa aacggaggtt tatctcagtg tactggaaaa cgtgctcgct
     1321 cagggcaaac aggcgctggt gatggtgccg gaaatcggcc tgacaccgca aactatcgcc
     1381 cgttttcgtg aacgttttaa tgcccccgtg gaagttctgc attccggcct gaacgacagc
     1441 gagcggcttt cggcgtggtt gaaagcgaaa aatggcgagg cggcgattgt gatcggcacc
     1501 cgctccgcgc tgtttacgcc gtttaaaaat ctcggcgtga ttgtcattga tgaagagcac
     1561 gacagctcct acaagcagca ggaaggctgg cgctatcatg cccgcgacct ggcggtgtat
     1621 cgtgcgcata gcgagcaaat cccgattatt cttggctccg caacgcccgc gctggaaacg
     1681 ttgtgcaacg tccagcagaa aaaataccgc ctgctgcgcc tgacgcgccg ggcggggaat
     1741 gcgcgtccgg caattcaaca cgtgctggat ttaaaaggtc agaaggtgca ggcaggtctg
     1801 gctccggcgt taatcacccg tatgcgccag catttacagg ccaacaatca ggttatcctc
     1861 tttcttaatc gtcggggttt tgcgcctgcg ctactgtgcc acgactgcgg ctggattgcc
     1921 gaatgcccac gttgcgatca ctactacacg ctgcatcagg cgcaacagca tttgcgctgt
     1981 caccactgtg acagtcagcg tccggtgccg cgccagtgcc cttcctgcgg ttccacgcac
     2041 ctggtacccg tggggctggg caccgaacag cttgaacaga cgcttgcgcc gctgttccct
     2101 gatgtgccca tttctcgtat cgaccgcgat accaccagcc gcaaaggggc gctggaacag
     2161 caactggcgg aagtacatcg cggcggtgcg aggattttga tcggcacaca aatgctggcg
     2221 aaaggtcacc actttccgga tgtgacgctg gtggcgttac tggatgtgga cggcgcgctg
     2281 ttttctgccg attttcgctc ggcagagcgt ttcgctcagc tttacaccca ggtcgctggt
     2341 cgtgccgggc gtgcgggtaa acaaggcgaa gtggtgctgc aaacgcacca tccagaacat
     2401 cctctgttgc aaacgttgct ctataaaggc tacgacgcct ttgccgaaca ggcgctggct
     2461 gagcgacgga tgatgcaact gccgccatgg accagccatg tgattgtgcg tgcggaagat
     2521 cataacaatc agcatgcgcc gctgttcctg caacaactgc gtaatctgat cctctccagc
     2581 ccgctggcag acgacaaact gtgggttctc ggtccggttc cggcactggc acctaaacgt
     2641 ggcggtcgct ggcgctggca gatattgttg cagcaccctt cccgcgtgcg cttgcaacac
     2701 atcattagtg gtacgctggc gctcatcaat acaataccgg attcccgcaa ggtgaaatgg
     2761 gtactggatg ttgatccgat tgagggttaa accgcttaca atgcgaggcg gatcgaaaaa
     2821 ttcaatattc atcacacttt tcatgaaaat tctgtaaccg ttttcacgcg ctatctgcta
     2881 aaaatgttgc cgatgtgaag taaacatgga tgtagtacgc ctgacgtgcc aggcgaggag
     2941 tgagtgtgaa agcgaagaag caggaaactg ccgcgaccat gaaagacgtt gccctcaagg
     3001 caaaagtctc tacagcgact gtctcccgag cattaatgaa tcccgataaa gtctcccagg
     3061 ccacccgtaa tcgggttgaa aaagcggccc gggaagtggg ttatttaccg cagcctatgg
     3121 ggcgcaacgt caagcgtaat gaatcccgca ccattctggt gattgtcccg gatatctgcg
     3181 atcccttctt tagcgaaatt attcgcggta tcgaagttac ggcggcaaat cacggatatc
     3241 tggtgctgat tggcgactgt gcgcatcaaa atcagcagga aaaaaccttt atcgatttga
     3301 tcatcaccaa gcaaattgat ggcatgttgc tgctgggttc aaggctgccg tttgatgcca
     3361 gcattgagga acagcgtaat ctgccgccga tggtgatggc gaacgaattc gcaccggaac
     3421 tggagctacc tacagttcat atcgacaatc tgaccgccgc atttgatgca gtaaattatc
     3481 tatatgagca agggcataaa cggattggct gtatagccgg tcccgaagag atgccgctgt
     3541 gtcactaccg cctgcaaggt tacgttcagg cgctgcgtcg ctgcggcatt atggttgatc
     3601 cgcaatacat cgcccgtggc gacttcacct tcgaagccgg aagcaaagcg atgcagcagc
     3661 tgcttgatct tccacaaccg cctactgctg tcttctgcca tagcgatgtg atggcgctcg
     3721 gcgcactttc tcaggcaaaa cgccaggggc tgaaagtccc ggaagacctt tccataatcg
     3781 gttttgataa catcgacctg acgcaatttt gtgatccgcc gctgacaacc atcgcgcagc
     3841 cgcgttacga aatcggtcgg gaagctatgc tgttattgct tgatcaaatg caggggcaac
     3901 acgttggcag tggctctcgt ttaatggact gcgaacttat catccgggga tcaacacgcg
     3961 cgttacctta aagtaaacct ttaagactgc cgtgactggt caaaggcccg ccgcttaagt
     4021 aacatggcgg gctgacgaac gaataaatac agcgaaacga tagtggcaca acgagattat
     4081 gtacgccgca gccaaccggc accttcgcgg cgaaaaaaga gcacctcacg gaaaaagcaa
     4141 cgaaatctgc ctgcggtttc tcccgctatg gtcgctattg ctgccgccgt tcttgtgacc
     4201 tttatcggtg gtctgtactt cattacgcat cacaagaaag aagagtccga gacgctgcaa
     4261 agccagaaag tgaccggtaa cggactacca ccaaaaccag aagaacgctg gcgctacatt
     4321 aaagagctgg aaagtcgcca gccgggtgtg cgtgcgccca cagaaccttc tgccggtggt
     4381 gaagtgaaaa cgccggagca actgacacca gaacaacgtc agcttcttga acaaatgcag
     4441 gctgatatgc gccagcagcc aacgcagctg gttgaagttc cgtggaatga acagacgccg
     4501 gaacagcgtc agcaaacgct acaacgccag cgtcaggcgc agcaattagc ggagcagcaa
     4561 cgcctggctc agcagtcgcg tacgactgaa caaagctggc agcagcagac gcgtacgtcg
     4621 caagccgcgc cggtacaggc gcaaccgcgc cagtctaaac cggcttctac tcagcagccg
     4681 taccaggatc tgctgcaaac ccctgcgcac acgaccgcgc agtcgaagcc gcagcaagct
     4741 gcgccggttg ctcgtgccgc tgacgcgcca aaaccgacgg cggagaaaaa agacgaacgc
     4801 cgctggatgg tgcagtgcgg ttcgttcaga ggcgcggaac aggcagagac ggtacgtgct
     4861 cagctggcgt tcgaaggatt tgactcgaaa atcaccacca acaatggctg gaatcgtgtg
     4921 gtcattggcc cggtgaaagg caaagagaac gcagacagca ccctcaatcg gttgaagatg
     4981 gcgggtcata caaactgcat tcggctcgcc gccgggggtt gaaaccctca aaatcccccc
     5041 catctataat tgcattatgc cccgtacttt tgtacggggt ttgtactctg tattcgtaac
     5101 caaggggtca gctcgtgaca actatagtaa gcgtacgccg taacggccat gtggtcatcg
     5161 ctggtgatgg tcaggccacg ttgggcaata ccgtaatgaa aggcaacgtg aaaaaggtcc
     5221 gccgtctgta caacgacaaa gtcatcgcgg gctttgcggg cggtactgcg gatgctttta
     5281 cgctgttcga actgtttgaa cgtaaactgg aaatgcatca gggccatctg gtcaaagccg
     5341 ccgttgagct ggcgaaagac tggcgtaccg atcgcatgct gcgcaaactt gaagcactgc
     5401 tggcagtcgc ggatgaaact gcatcgctta tcatcaccgg taacggtgac gtggtgcagc
     5461 cagaaaacga tcttattgct atcggctccg gcggccctta cgcccaggct gcggcgcgtg
     5521 cactgttaga aaatactgaa ctgagtgctc gtgaaattgc tgaaaaggcg ttggatattg
     5581 caggcgacat ttgcatctat accaaccatt tccacaccat cgaagaatta agctacaaag
     5641 cgtaaggatc tcccatgtct gaaatgaccc cacgcgaaat cgtcagcgaa ctggataagc
     5701 acatcatcgg ccaggacaac gccaagcgtt ctgtggcgat tgctctgcgt aaccgctggc
     5761 gtcgcatgca gctcaacgaa gagctgcgcc atgaagtgac cccgaaaaat atcctgatga
     5821 tcggcccgac cggtgtcggt aaaactgaaa tcgcccgtcg tctggctaag ctggcgaatg
     5881 cgccgttcat caaagttgaa gcgaccaaat tcaccgaagt gggctacgtc ggtaaggaag
     5941 tggattctat tattcgcgat ctgaccgatg ccgccgtgaa aatggtacgc gtccaggcta
     6001 tcgagaaaaa ccgttatcgc gctgaagaac tggcagaaga acgtattctc gacgtgctga
     6061 tcccacctgc taaaaacaac tggggacaga ccgaacagca gcaggaaccg tccgctgctc
     6121 gtcaggcatt ccgcaaaaaa ctgcgtgaag gccagcttga tgacaaagaa atcgagatcg
     6181 atcttgccgc agcaccgatg ggcgttgaaa ttatggctcc tccaggcatg gaagagatga
     6241 ccagccagct gcagtccatg ttccagaacc tgggcggcca gaagcaaaaa gcgcgtaagc
     6301 tgaaaatcaa agacgccatg aagctgctga ttgaagaaga agcggcgaaa ctggtgaacc
     6361 cggaagagct gaagcaagac gctatcgacg ctgttgagca gcacgggatc gtgtttatcg
     6421 acgaaatcga caaaatctgt aagcgcggcg agtcttccgg tccggatgtt tctcgtgaag
     6481 gcgttcagcg tgacctgctg ccgctggtag aaggttgcac cgtttccacc aaacacggga
     6541 tggtcaaaac tgaccacatt ctgtttatcg cttctggcgc gttccagatt gcgaaaccgt
     6601 ctgacctgat cccggaactg caaggtcgtc tgccaatccg cgttgaactg caggcgctga
     6661 ccaccagcga cttcgagcgt attctgaccg agccgaatgc ctctatcacc gtgcagtaca
     6721 aagcactgat ggcgactgaa ggcgtaaata tcgagtttac cgactccggt attaagcgca
     6781 tcgcggaagc ggcatggcag gtgaacgaat ctaccgaaaa catcggtgct cgtcgtttac
     6841 acactgttct ggagcgttta atggaagaga tttcctacga cgccagcgat ttaagcggtc
     6901 aaactatcat cattgacgca gattatgtga gcaaacatct ggatgcgttg gtggcagatg
     6961 aagatctgag ccgttttatc ctataatcgc gttcaatcat tttcatcatt gtttgatggg
     7021 gctgaaaggc cccattttta ttggcgcgta ttatgactga acaacaaatt agccgaactc
     7081 aggcgtggct ggaaagttta cgacctaaaa ccctccccct cgcctttgct gcaattatcg
     7141 tcgggacagc gctggcatgg tggcaaggtc acttcgatcc gctggtcgcc ctgctggcac
     7201 tgattaccgc cgggctatta cagatccttt ctaacctcgc caatgattac ggcgatgcgg
     7261 taaaaggcag cgataaacct gaccgcattg ggccgctacg cggcatgcaa aaaggggtca
     7321 ttacccagca agagatgaaa cgggcgctca ttattaccgt cgtgctcatc tgtctctccg
     7381 ggctggcact ggttgcagtg gcatgccata cgctggccga ttttgtcggt ttcctgattc
     7441 ttggcgggtt gtcgatcatt gccgctatca cctacaccgt gggcaatcgt ccttatggtt
     7501 atatcggtct gggtgatatt tccgtactgg ttttctttgg ctggttgagt gtcatgggga
     7561 gctggtattt acaggctcat acattgattc cggcactgat ccttccggcg accgcatgcg
     7621 gcctgctggc aacggcagta ctgaatatta ataacctgcg tgatatcaat agcgaccgcg
     7681 aaaatggcaa aaacacgctg gtggtgcgct taggtgaagt gaacgcgcgt cgttatcatg
     7741 cctgcctgct gatgggctcg ctggtgtgtc tggcgctgtt taatctcttt tcgctgcata
     7801 gcctgtgggg ctggctgttc ctgctggcgg caccattact ggtgaagcaa gcccgttatg
     7861 tgatgcggga aatggacccg gtggcgatgc gaccaatgct ggaacgtact gtcaagggag
     7921 cgttactgac taacctgctg tttgttttag ggatattcct aagccagtgg gcagcataac
     7981 tgacaaatat caattaacaa ttgatgattt tgccaacagc ccacatagcg cgatatactg
     8041 aaaattctcg cagcaactga atgttaagcc tatgaaatac gatacttccg agctttgtga
     8101 catctatcaa gaagatgtta acgtcgtgga accgctgttc tccaactttg gcggacgggc
     8161 gtcgtttggc ggacaaataa tcacggtaaa atgtttcgag gacaacgggt tgctgtacga
     8221 tctgctcgaa cagaatggcc gtggtcgtgt tcttgtcgtc gatggcggtg gttctgttcg
     8281 tcgcgcactg gtcgatgctg aactggcgcg tctggcagta caaaatgaat gggaaggtct
     8341 ggtcatttac ggcgcggtgc gtcaggtaga tgacctggaa gagttggata tcggcatcca
     8401 ggcgatggcg gcaattccgg ttggtgccgc tggcgaaggc attggcgaaa gcgatgtccg
     8461 cgtcaatttt ggcggtgtca ccttcttctc cggcgaccat ctttatgccg acaataccgg
     8521 gattattctt tctgaagatc cgctggatat tgaatgatag aaaaggcacc gcaaggagat
     8581 ttaacacacc ttactcccct tgatgattta cttatttttc aatgactaaa tcaaaacaag
     8641 gggagcaaat taacgcaaat tacaactagt caatggacag tttatggaca ctatcagcca
     8701 gcggattaaa gctaatcgca tcctgtaaaa agtccggtga aaaatgcgca tacgtcatcg
     8761 tttgttgaat cgtggcgtgc ccaaggattc gctgtaacgt aacgatattt ccgccgttca
     8821 tcatgaaatg cgtggcgaac gtgtgacgca taacgtgagt cgcctgacct ttgggtaaat
     8881 ccggtttcac ctcctgaagg atatcccgaa aacggatgta atcagcctgg tacaatagcc
     8941 cggtttttcg ggtttttatc aacgaaagca ccgtatcaga aacaggaacc gcgcgggatt
     9001 ttccattctt tgtgttaaag aacattacgc ggttaccaac gatatgctca ccacgtaagc
     9061 cagctgcttc cccccacctt gcgccagtgg aaagacacag aacagcaaca cgacgcgcat
     9121 ccccctcgag tcgttcaagc agccgatcaa tctcatcatc tgaaagaaag gccatttctg
     9181 tattcctgac cttgagctta cgtattccac gcaccgggtt ttcgttatgg aaaacctccg
     9241 catcaatcag cgaggtaaac attgcagaca aggcacacaa atcacgatta acactggagg
     9301 gcattaatcc ggcctgtaat ttatctgaac gatacccgag cataaacttt cgcgtcatct
     9361 cactggcgcg ggggtccctc atctcacggt ttattttttc cagcctgacc ctgtaagtat
     9421 ccccgtaagg ctgattccgg ccatcaagca cccaccaggc atctatcagt tcggataacc
     9481 gacgctgatc agctggcttg gcctgccacg gtttatcgtg gaaattctgc aaaacatacc
     9541 tttcaaactc ctgtgccttt gacttcaggg taaaaatctt acggatccgc tttccttccg
     9601 tcccttgcgg tcttacatca accttataac gcccgtcttt gagttgctta atcgacatga
     9661 ttagcccctc caacggatga atcagccgta cactctctac gggatgaaac ccgctcgatg
     9721 tacagcgtta gccagcattg cgacgagatc ggggtgattt ttgggtaatg ccgattgaaa
     9781 cctgatcgcc ctccgactga atggagccat caagagagag agccggggca acctgcccgg
     9841 cagcgggaaa tgttttgtcg tgaagtatcc acatagtgta tttttcgaat cgctcgactt
     9901 ggattacttt ttctacaacc tcaattcgcg caggtttata tccagactca taatttccta
     9961 tagttcctac agaaaccccc gttacttgcg aaaactgaga ttgtgtaagg ttttccgcgt
    10021 ttctgattgc ctttagtttt ttggcgtaat ctcttgacat gttcaccata cgatgagtat
    10081 cctctattcg aacttcacca cagggtgaag taaacaaatc aaaacaaacc ttcatgatgc
    10141 gcgacaagcg caccaacgca agaggatagc aaatgcggaa gtcaattgcc gataaagagt
    10201 tatctgacac acaagccctt caggaaaacg acagcatcac cgaatccgtt ggcgcggcaa
    10261 acgacgatga acaccaagag gaaaagaaaa aacgcaccta caaaaaaggg gcaaccttgc
    10321 gactggatgg cccggtagcc ggactttgct cacttgaaaa aggggcagcc tatatcgggt
    10381 tgacaaaagc tgcattgcgc gtggcgcttc agcgaaatca gatgcctgga cacaagaccc
    10441 gttcaaaccc tgaagatgaa aattcggatg gcgtatggtg gtttaacgcc agagagtggg
    10501 acaagctggc tgacgaacta ccagaatgcg agccgccaga atggcacaac tggaaaagct
    10561 actggacgta cgaccgacag aaaagaaagt tttccccggc aaacaaagaa gactgccaga
    10621 ccatcaatgg aaagcgggtt tacacaggaa gaaagtcaaa actggagcaa caccaataca
    10681 gcgaaggcaa ccagtgacat caaaacaaac cgggatcatt aagagcatcg ggatttgaag
    10741 gtgttgacat gactgactcc actcaaacaa aagacttata cagcgattta cgcttcatca
    10801 tcatacaaca gcaatcaggc aggatggatc caacacgtgc aagcaaggcc tggaaagaag
    10861 cccttaccga tcaggggatc gatgtaatta gcgaagtcat ggctgaagtg atttttaggc
    10921 tgacaagaga cgaatcactg taatcatcgc ataaggaccg agtgaagaat gaagcaacaa
    10981 cgaaatgcac gcatcaataa caacaccgaa cgccacgttg cacgccagca cttccgtaaa
    11041 aatagtgcga ccgcaaccac tgaaatccat accacaccag acggtcaccc ggtaaaacaa
    11101 accggaaagc acacctgggc gattggcgac actggcatcg ttattcacaa aagttttcgc
    11161 agccctgtta ctggcagatt taatttcacc ctgactcgcg gggatgatta cttcggacag
    11221 gacttcacat tttttgaagc cttaagaacg gcagacagat taatcagcgg cctgcgtttt
    11281 caatatccgg gaagcaagca ctgataaata aaggattcag ccatgatcga agcctacaaa
    11341 atacttatca ataacgcgtt gcaacgttat cactttgaac tcgcaaaccg gggcgtaact
    11401 aaggatgaaa gccttcgtaa ctcactgcgc ggcgcaatta tggggctata caactgcgca
    11461 ctgggcagcg atgaccgtga agcgctggaa gaattaaagt cgattatgac agaactgttt
    11521 gaaggtgaaa ttgttgagcc ttacttcaca ggggtagccg catgagcata tttctttcat
    11581 gggtaattct gattatctcc gtgcttatgg caatcggcat tatgcgaatt atccacgaac
    11641 tgaaggatat cgaattattt tttactggcg acaaacgccg taaataaagc gaaaacttta
    11701 tgaagaatgt aaaagtaata ctcaccatcg ctactgcatg ggtgatattt caggtgagtg
    11761 cgcttatctg gttagcactg aagaatctgg ggataatctg aagccgaaag caggcgcgga
    11821 ataacaccag aaaacacacg agcaattaat taaggcattc atgccttcgg gttccctttg
    11881 tcttttttca ggagtcaaca aatgagaaca gccgaatcac ccggcagaaa aataaccgcc
    11941 agaccagcta accatcatgc acatatcagc atggccgcaa gaaaagccga gcgtgcgcat
    12001 atctgcgaat tactgcgcga gctgactgag acagcgaaag agacacacac ctgtccacag
    12061 gaaacctgcg cagccattga acgactgatt gcaatactcg ccaacccaga cgaactgaag
    12121 gggtgcgcat aatgaaccat ctcatgattg acaccgaaac actgggtaac ggaccggacg
    12181 cagccatatt cgcgatcggg gcggtgtttt tcgacccgtt caccggaaag ctgggcaagc
    12241 agtttgagaa attcatcgac ccggtggatt cagaacgcaa cggcggaacc gtcaatgcag
    12301 ccaccgctgt atggtgggcc ggacaatccg tggaagccag agcctgcctg cgcaacgcag
    12361 atggaacaga gctggcagca gtgaccgaat ttctggcgtt catttcacgc aatatgcatg
    12421 acgaaagccc gggcaattca ctgaccatat ggtgcaaagg cgcatccttc gatttcccaa
    12481 tcctgaaatc cgccataaca cgcacagccg gagaaaaaag catcccctgg tgctactggg
    12541 acgagcgctg tatgcgtccg cttatcgcca tggctgaatc aaccggctgg agaatgccag
    12601 gccgatcagg caaagagacc attgctcaca ccgcgctggg cgacgccgtt taccaggcaa
    12661 aggtcgtgtc agaaatctgg cagcgcttca caacaccatt cctgaacata tgaggtgaag
    12721 ccatggccgc catcctgaat gtgtgttgct gccccccccc tgaacgtgga aagaaaagcc
    12781 cgcgcatcgc tgcgtttatt ccgcgacggc gtgcggaatt tttcacgtat aaaaccccac
    12841 gactacctgg taataagaat cggttttcgc tggcgattac tgagcaaaaa caacgggaag
    12901 tcatgggatt tattaacgca cgaaacctat aacaaagagg ccgcgttatg agcaaagaac
    12961 tgttatccgg cacaccgctg aaatgggtgg gtggcaaaag ccgcatcatg aacacactga
    13021 aggcgcatct gccagaagct gactgcctgg ttgaaccgtt cgtgggtggc gcatcggtat
    13081 tcatgaacac cagttacaag cgttacatcc tgggtgattc caatggtgcg ctgattaatt
    13141 tctggcgggc agcacgggac aacaccgaca gtctgatcga cgaggcgaaa accctgttcc
    13201 tgaaccacaa caacaggggt gactatctca tcattcgcga aacctttaac gcagagaatc
    13261 atgccttcct gagtagcaga acgggaataa gcgaccgccg tgaactgcga ctggccgcca
    13321 tgtttttgta cctcaaccgg cactgtttta atggcctgta ccgggtgaac ggaaaaggtg
    13381 attttaacgt gcctttcggc ggctaccgaa aaccctactt ccctgagcgg gaaatccgcg
    13441 catttgcaga caaggcaaac agcacccaaa cgctgcttat ccacgcagat tttaaggata
    13501 cgctgagctc tgccagtcac ctgttcggca tgggcaacac gctttgcgtg tactgcgatc
    13561 ccccttacct gcggttcagt ggcgagggca atttcaccgc ctacggaaaa ccattcgatg
    13621 aacacgagca cgttcgcctg agggcagcac ttgaccgcct ttcgcaggaa acgggcggca
    13681 tgaccagaat caccatctcc aattccgaca caccggaaac cagacgcatt tatcggggtt
    13741 accggatgaa ctgcatacag gcaccacgaa gcgttggagc aagaacccgc gaaccagcac
    13801 cggaagttat cgccaccctg agacagtgca acgcctgcgg tcactatggc ggtggctact
    13861 gcccggactg cggacctgtg acgggagatg caacttacag cgtgatgttt acccctgcca
    13921 gtacgtgaat aaacagatga atcagtaaca ccggagtcag aaaatgaata gcgaaaacaa
    13981 caaagagctt ttgcgggaat tgcgtctcat cgcccgtgca cacgaggaaa cagcaaaagt
    14041 tgtcgcagct gctggaagac cagagctcgt tatcacttca gcaatcttac tgaggatcac
    14101 catggaagca atccgggtga ttcaggatca ggaaagtgaa atcgccgccc ttaaaaagca
    14161 gctcaacgag aaatctgccg gacaaagggg tcagcaatga tccaggagtc aaccgttatc
    14221 agattcacag ccaacggccg tcagtacgag gtagacgaaa gcctgattga ccccggcatg
    14281 acacgccagg gctcacgcaa tccagagatg catcacatcc gccttatcaa cggttcgcac
    14341 ttctgcgcca caaacgtgga agaagtacgc gtgctgacag caacacgcaa tacgggaggt
    14401 aaagcatggc agtgatttat atcgccggtc ccatgaccgg atgcaaagac tacaacagga
    14461 cggcattttg cgcggaggac ctgaaacttc ggtgcgccgg aaacatcgtt cttaatcctg
    14521 ccgtgctgcc tgacggatta agccagcagc agtacatgag catttgtatt ccgatgctca
    14581 tgtgtgccga tgccatttac ttactggatg gatgggaaga gtccgctggc gcacgggctg
    14641 agtacgcaat ggcactcaag ctgaacatgc cggtgtcatt cccggaaaat cgggcggccg
    14701 gcgaaactgt cgcacagctg tttggcaaat caaaagccgc acaggcgact gagtaaccga
    14761 ccggagatcg aaacatgatt gcgcgaagag ctgccaaccg cctcacaggc tttattccag
    14821 gcaagattaa tcatgcactc catgatggga ctgtcacttt cctcaacaaa ggagaggcgc
    14881 gcaatcatgc attccttact aatcacatgg cgctgactga taaggcgctg gtaactgact
    14941 gtctgccatt cagccttttc agccctgcgt tcaaaactgg cagccatgca ctgccagagc
    15001 agaaacagaa ccacactgaa gagtaccaac acaacaaaat cacacaggac aaacgccgca
    15061 acaccaacca gaaaggcgct gattcgcaga gcagcagaaa cgcgtcggta aaacacacat
    15121 gtaatacgtt caagccagta gctgtaccac aggtcaaaaa gaatgtcgta atcagaaggg
    15181 agcttatcac tcatcagaaa caccccgcgt atgcagacaa tcaccagttg ccaggtgata
    15241 ttaattcgcc acattctaac cacatcagca cgcaacacaa aaaccgcttg ccagcgctcg
    15301 tcgtgtcagg ttacatttcc gctgcacctc ataaaacggg tgccgggatt ggaaccccgc
    15361 cgactagcaa agcgcacaac cgcgccagcg gtttttttgt gcgtactgta tcgccacgtc
    15421 tttttcgcgt cagaataatg gcggggcgta cggggccgac ttcggtcggg ccgggttctt
    15481 tgctagccgg tagttccaac cctgtacgtc tcgccaccct gagcttggaa cctcgggttg
    15541 gtgagttttc agaacttact agcaaagagg ccacaccatg gcaaaccgca agcaacagcg
    15601 cgcccgcgct gagcgtcttc acatccgcag cgaaatcaac cgcagacttt tccgcgcaac
    15661 acgcgtcgcg caaatcatgc atatcaacat gctgcatgag cgcacgcacg cactatcaaa
    15721 ccgttactgc gctgccgtct tcagctatct ggccgaagac ctggcagaac tgcaaagcct
    15781 catcaaccag caccgataaa ccacatccgg aagctatccc acacctgcac gcgggaggct
    15841 tccgcacatc tgttaccgga ggttctcatg aagagcgtta agggactttc agatattcag
    15901 gacttcttta actggatgtg ggcggtggat cccctgctgg cttcccgcct gaacaactgg
    15961 cacgactatt accgccgcgc ctgtaaacag gctgcccgca gcgtcaccac atacggggct
    16021 ttctccttca ccgttgacta tgcagacaat caccagttgc caggtgatat taattcgcaa
    16081 cattctaacc acatcagcac gcaacacaaa aaccgcttgc cagcgctcgt cgtgtcgggt
    16141 tacatttccg ctgcacctca taaaacgggt gccgggtttc gcagcctgcc gactagcaaa
    16201 gcgcacaacc gcgccagcgg tttttttgtg cgtactgtat tgccacgtct ttttcgcgtc
    16261 agaattatgg cggggcgtac ggggccgact tcggtcgggc cgggttcttt gctagccggt
    16321 actgcgaacc tcgtacgtct cgccacccac agtttcgcag ctctggatgg tgagttttca
    16381 caacttacta gcaaagaggc cacaccatgg caaaccgcaa gcaacagcgc gccagcgctg
    16441 agcgtcttca catccgcagc gaaatcaacc gcagactttt ccgcgcaaca cgcgtcgcac
    16501 aaatcatgca tatcaacatg ctgcatgagc gcacgcacgc gctgtcaaac cgttactgcg
    16561 ctgctgtctt cagctatctc gccgaagatc tggcagaact gcaaagcctc atcaaccagc
    16621 accgataaac cacatccgga agctatccca cacctgcacg cgggaggctt ccgcacgtct
    16681 gttaccggag gttctcatga agagcgttaa gggactttca gatattcagg acttctttaa
    16741 ctggatgtgg gcggtggatc ccctgctggc ttcccgcctg aacaactggc acgactatta
    16801 ccgccgcgcc tgtaaacagg ctgcccgcag cgtcaccaca tacggggatt tctccttcac
    16861 cgtggacggt cgttaccgcg tggacgtgaa aggtgatggt gatggactgg gtttatttca
    16921 catcaacggc accgacagta ccgccatcag ctggtcgtca tcagagatgg cgctgattct
    16981 ggaaatgaca acccacagcg tggccgtgaa aggtatatac accgcagaag gttttatcgg
    17041 cgaataccag cgccttctcg tgctgtaccg cgcggcgttt caggtggcaa ctgccgggga
    17101 gtgcgacgca tgagcgacca taacgcacaa ctggcgtacc cgtggaacgc cccgctaccg
    17161 gttatcgatc ctgaaacgtt cggcagagca cagccaaccg cattacgcca ggccattcag
    17221 agctacatca acgaggacat tcgcattgat gccagactgg atgaagaaac cgtcgccttt
    17281 ctgacaacca cagacgaagg gaagcgaatt aattcgcacc ttcaccacaa cgaagagcgt
    17341 cggatcagac tggaaaaact ggcgaagcac aaacgcgaga atccgcccac ggtggtatcg
    17401 gaagccatgg cagaacttcg cgccctgccc tcgtttcttg gcaacgtgct cattcgtgat
    17461 ctggcgcgtt taaaccgcaa acaacaaagc gcccgcaatg aaggcgtgaa aaacaacaac
    17521 actcacgtgg ccgataactt cgttcgccgt ggactgcgca cacgcctgaa acgcatcgaa
    17581 caggtgaatg aatgcttcgc cacgacggca ttcaaagcca ccgcagcgcg tcatcgcctt
    17641 gatgaattac tcatgctgcc acaactcaac cgtgatcaaa ttcagcgcct tgccacacta
    17701 acggcggcgg cattttccac cgaactggaa cgcatatgtg atgaagttac tgagcggaca
    17761 ggaaaaggtg acgataacct gttcacctgg ttgctgactt accagcagct ggcgcgcatg
    17821 gcgttaaaac tgggcgtgac cccaccatac tggccgtcac tcgagatcag acgtgaccgc
    17881 cgcacggcac cagaccctga actggtaccg ggtgcggtaa tgcgtatcac ctgcgccacc
    17941 tggtggaaca accagctgcg ccacctcgct gatctctggc gggaggaatt actgcgtgcc
    18001 gccggaagag tatcgcgcaa ggcatccccc tacatcagcc atgagtcact ccaggaattc
    18061 cgggagaaac gccagcgcac ccgtgatttt ctcaaaagct gggacattga aaacgaagac
    18121 ggtgaacgcc tgagtctgga ggacgtgtac tggtcagggc tggggaatcc gcgcaaccgc
    18181 cgcaacgaga tgatggcctg cgtcagaggg atggaacagg tcgccgaatc gcgtggtgac
    18241 tccgcgttct tcgtgacagt gacctgccct tcccgctttc acagcgtaaa cgaggacggc
    18301 agcctgaacc cgaaatacaa cggtgccacc gtgcgcgatg ccagtgatta tctggtttac
    18361 gacgtctttg cagctgcaag aaaaaaactc aacaaagaag gcctgagctg gtacggggtg
    18421 cgcaccgttg agccacacca tgacggcaca ccgcactggc acatgctggt attcaccgcc
    18481 ccggacaatg aagaacgcat cattgaaatc atgcgtgacg cagcaatcaa atcagatcgc
    18541 gcagaactgg ggaatgatat ttctccgcgc ttcaagtgcg aaaaaatcga cccggcaaaa
    18601 ggcacaccgg caagctacat cgcaacctat attggcaaga acctggacgc cagcaccttt
    18661 cacgacaatg acccgaaaac cggcaaacct tacgccgata aagaatcggg aaaaaccatg
    18721 gcggaaaccg tggaaaacgc cattgcctgg gcgagtctcc accgcatccg gcagttccag
    18781 ttcttcggta tcccgccccg ccaggtatgg cgtgaactcc gccgccttgc cggacaaatg
    18841 gagcgtaacc ccgcatcacc gaagcgcctg gaccatgacg acattgacgc cattctggcc
    18901 gccgctgacg tggggtgttt tgccacctac atcaaccgtc agggcggcgt gctcattcca
    18961 cgcaacctgt acctgatccg caccgcatac gaaaccgcag atgaggccaa cgactacggc
    19021 gaattcccgc agcgcatcta cggaatttgt gccccgtcgc tcggcgagcg ctacaccatc
    19081 tgcacccatc cggacgagtg gaaactggtc agaaaagaag cgaaagccga caacagcacg
    19141 gacgaggatt ttgacgttgg gggcggcttc gccgcacctt ggactcgtgg caataactgt
    19201 ccccatgatg aaaaaacaga caccggagag ggtaaaaccc caccaccgga aaccaggaaa
    19261 gaacaaaaac tgaccatccc ggaaggtgat ttgggcgaat ggttaaggtc actgaccccc
    19321 acagagagaa aacaactcac caggcagctg aaagatgcgc catctgacac aaaaaccgtg
    19381 aaaaccggcg gctccaccac gcccgatgac gtcatacatg tacgtacagc attgccgctg
    19441 actgacgtca ccaccaccgt gatcaagtcg gcactgagca cgacgggcga ggagctacac
    19501 agtggcgcag cactttccgt acacagaggt gcacgcgttc gcctgcctga cgggcggatt
    19561 gtgcactggg atgaaaaatc acgaagcctg gtggcagcag atcaaccgga ggttaaccca
    19621 tgacacgatt ccattgcgta ctccagcgcc atgatttcag cacgatcatg aacgcacgca
    19681 ttccggcact gacactgagc ggcgtcagtg ttcgaccgca gccaggcgac gaactggaaa
    19741 tcacgatcgc cagtcccgat cgcagcacag acaccgttat gaccgacatc ctgactgcaa
    19801 gaatcaacac cgacagaagc gcattcatcg ttgccgtgcg ccctcgtcgc gccatatcag
    19861 agcctgaacc actgactccc gtaggaaaaa tcatgcaggc ataccacgaa aaacagacca
    19921 gaaaactcaa cgaagaactg gcaagaacgc tggccgtcgt taaccgaaaa gcggcaattg
    19981 tcagctatgt gcattcggcc gccagcaggc aatcacgcag gtaacaaaag accatgaaca
    20041 tgaccagaaa cagcaaaccg aaggagcaca ccatgaacga catcaggagg atcagcgaca
    20101 aagagcttac gaaaatgatt gagtcggcgc agcagctgct ttcacgacga aatccggttg
    20161 tggaagaaac atggtgggaa aatttactgg cagcactgac cgaactccgc gaacgccgga
    20221 acaacgaagg agccgccaaa tgaagacacc cagaatgacc ggaaaggtaa tcattttacg
    20281 ctacctggaa acgcacgcac gcttcagcgc gccagagctg gttgaaaaac tgggcgtgaa
    20341 aaggaccatc attaatcagg ccgccggaaa gatggtacgc gaagggctgc tggttgtgga
    20401 cgggtacacg gacaagataa cgcattaccg gaagccaacg gaaaaggaac gactggagct
    20461 ggagcggcgg gctgaacagc aacaaaagcc gtccgtaatc caggactgca agcgaagcga
    20521 aatcatgaag cgaattctgt ttatctacgg cgctggcgaa gagctgccag tcatctaacc
    20581 ccaacaataa aaccaacacg ggagacaaat atgccaacac aggttgcaac agagggacga
    20641 ctggcagcag acccgaacac aaggagaaca accaaaggaa ccaacgtcac aacggcacaa
    20701 ctggcggtat cactaccctg ccatggcgca gaacagccaa ccacaatgtg tctttacctt
    20761 atttcattcg ggaagcagca cgaagaactg gcaaaacaca gaaaaggcga tctgattctg
    20821 gtgcacggaa acttgcaggt aaacaggtat caggacaagt acgggcaaca gaaagagggc
    20881 tggcagctgc tgacgcaaag cattatcagc tcaagaaccg ggcgatgact tcacgcatgt
    20941 taccctcagc agcgagggta acacaggatt tcttttttca gtcaaaccga aatcggccgc
    21001 ttgtcgcgta tgcataggtg accaccgcac ccagcgcatt cacacgcttc ttcacataaa
    21061 tcacattacc atcctgatag tcaccgtatg ggtagcaaat aaagattgtt ttttgcaaat
    21121 caggatcatc aacaaatgcc cctgaacaac tttttgagct accgcgactg tactcatagg
    21181 gagcggaata gaatgaatct tcacccggaa gaaaatatat cagcgtaacc aggagggtaa
    21241 acagaatcat ccaggtaaga aggcacatac ccgaaaaaaa cttaagatac gatgtaaccc
    21301 cggtaaattt agaccgatca accaggttct ttttcaacag gcacagtgag acaaaaaccg
    21361 aaagcaccac agacactgaa acaaggcagt ttgtcgtgaa cgaataaagg taggtaattc
    21421 tgtaaacatc gtttcgccac caaataaaaa gaaaaatccc ccccaaaaca gaaagcgtga
    21481 caaaagttaa ataaggatat gagtaatttt tcacagttct cacccggcaa tacaccactg
    21541 attcagacag atgattatca tcaccaccca cccccaaaaa tcgcaagata aaaaatgcac
    21601 aaaagcgcac gaaattgcac aatttttctt atgtgtttca tccccattca gcccagtaac
    21661 ggcgctggct gaggccggtt tgcacggtgc acgaaaaacg aggcaattgc agcgcgcagg
    21721 cgacgaggga agggcgcccg aattgaggga tggaaggcgg gttgctttat ttggggtcgt
    21781 atggcgcgtg atacgcacgc agtaaagtca gttttgaaaa accacatcaa aacatggtca
    21841 gggaaacagg ccgtcagaat gcgtctgatt gcgtctggcg aggtcggaga tcgggaggga
    21901 aacggacgtg acgaacggga taaaaaagag agacaaaaat cactgaaatc ataaagttaa
    21961 tagaatttaa agtatcagtc attcacgggt gaaaacttgc gcacaatatg aggtgagcgc
    22021 agaatacagg ctggcataaa cagaccaaag gagagcgaca tgagccattt tcgcgatttg
    22081 tacctggcaa acaaaaagag ccgggacgcg aggcgggagg ttacagggat tctgcgcaat
    22141 caggctgaaa agctggttgc tttttatgag cagtggctgg aattgccttc agagacatgg
    22201 gaagacaaag acggcgagca tcatcgttac gttgatacgg ggtttccgtg cagaaacgat
    22261 cgggacttca ggccatttcc ggtaaatatg attgcaaccg acacagataa catattccgg
    22321 atggctgtaa gaacatgggt tgactcacac gatgaaggag agtcggtaaa catagtcctg
    22381 gcaatggagt tacattccgt cggaaaagac ggtgtatctc ttcgggttcg cgtggaagat
    22441 gatacctcca ttcgcgtggt catcaacgga gacggtgaca gggaatggct gccgatagta
    22501 aacagtatga agatgcatat tatcagcaag ctgcaaagaa gaacgccatc cgcattccgt
    22561 taagagagct ggagagtcgg ggcataaatt ttttatgccc cggcgaagca gcagacaagc
    22621 gaagcgcgtc agctggcgtt atccagaagc agggcatagg ggttaaagcg catcacctca
    22681 acgccaagcc attcattgac atgcttcatg gcctccatga caggttgcaa ctcgttgatg
    22741 aaaaacactc tcgccgcttt ttcaacgtca ccaaacgccg cattcccgtc aggcattgcc
    22801 cccatcagtt gaggtggtac acgatgggca gcaaggatat cgtcgcgggc gctccccttg
    22861 atgttcagga attcatcctt tgcggttatc tgctggaacg gcagtatttg cacaccatcc
    22921 ttgccaccac ccggtgcatg tagaaacagg tttttaaacg ccccgcgacc acgggaatcc
    22981 gtaagcgttt ttttaatggc ctcgacactt tccgcattcg cctgagatgt gcctacatag
    23041 ataatacacc cggcatgcga gccgttatcg tagtagagtt tgcggaatgt atccgctgag
    23101 tttgacaggc tgacagacag caacccgccg atatattccg gcatcccgta gatctcctga
    23161 ttaatatcag gattaagcac gtggcacaca gtccctttcc tgaaactgaa ctccgtaccg
    23221 tcgtcgttga gataccagta ggtatccagg tcacttcccc tgcgtgtata ttttgccagt
    23281 gccgggcgaa gcaggagtgg ttcccccagc ctgttacgcc gcacttcaag ataggcatta
    23341 ccaaacacaa accagtcgag cgcaaacgct gaaaacgcct ggcgggacag cagagggtga
    23401 ggaacaaagc acccgttcag ggcgttgcgc ttgaagtaca aagccgactg atgccaggac
    23461 gcacgacggg atgcacgggc gatagcgttc cagtccatgg gggtatcaaa gtagcgcccg
    23521 ttatctgcac atgacatgtt atccaggaga tccatgcggc tgacttcata gggcccatca
    23581 aaggtgaatg cgctgagtgc aggcgttgat ttcagtgcat cgtgtagcga atcctgcccc
    23641 cgctttccgg tgtatttttt gcgtttactc attgtgttca gaactccatt gcaaagccgc
    23701 gattatcctc acgctccgca ccaattggct cattgataac agcaagcatg gttgcccagg
    23761 cgaggtcacc atggctgacg ccacgggagc ggtcggtgtc gtaggtgata tagccgccgg
    23821 gtgtctgtat ttttcgcacc gatgcgaatg cctggatcag gtcacgttca ctgcggtcat
    23881 actcccagcg tccggcacgc accacctgaa gcattttcat gaccagtgcc cgcttggagg
    23941 cgatattgaa gttgtacggt acggccatcg ggaaaaactt tttcacaatc tgataaaccc
    24001 cctccccgtt cccgccggtc acatcaatgc cgatatgttt cacgttatat ttttgggtgt
    24061 attcctcaat cagggccgcc tgctgttcaa actccatacc ctgcaccctg accgtctcca
    24121 cggtacgaaa acgaccgcct gtaacccgtg gaggtacgca gaccgacagc gccccgcagt
    24181 cccccttccc gctgctgccg ttggcgtcgt acccgagcca gacttcccgc acgcccatcg
    24241 gacgcgcagc aaaaggtttc cagtcaggcc attcgtcgta tccatccaca ccacagccga
    24301 tcagcgcatt cagactgaac gccgcctcac catcacgcac gaactcgcac atgtacaggt
    24361 tacgaaattc gctttcggtg ttttcatccc ggatatcgtc aatgctcgta tattcccacc
    24421 cgttcgcgac tgcatcctca atggtgacaa tctgccgcca tgttttatcc gggaagagca
    24481 cgcccccttt caggttcgaa tgagacacat cgaattcagt gcgttttgag cggggacgct
    24541 cactgttcca gcgatccccc gtccagaact gataagcttc atgcgtttca cccgatggcg
    24601 ttgaaaagta ggtacgggta agccctttca gggtcgccat tgcgccagcc acctttcgca
    24661 actgcacaaa attactgacc cagaaaaatt catcaaattt aaggtgtccg gtatacgact
    24721 gagcggttgc tgccgatgtg ccaaggaagt gaatctctgc accgttagac aaaacaatct
    24781 tgtcaccccc tttcagttcg acatcaactt ccagcgccgc ctgctgaata aagtgtttga
    24841 actgatacgc ctggcgacgg gatgcggaca aaaatatctg gttgcgctgg tacccggcgg
    24901 agacatcatc acgcagtgca tcaaggagtg cttcgcgcgc aaaataccag gtcgcaccaa
    24961 tctgacgcga cttcaggatc atgcggttgc ggtgatggcg gttgtcgtac cattcgcgct
    25021 gatgccaggc cagtgacggc aggatttttt ccctgagtgc gacaatctgc tcttcggtga
    25081 agtgattttt cagtttgcgc ctgcggggct tcttaccgga aacagcctgt tctccctgac
    25141 cgtcattcag ttttttcagc tggcgcgtca gcaggtcaat ttccttaaag tcaccgccgc
    25201 ttttatccgg tttggcggtg agctgtatca ggcgtgcatc aatggactgg gtgacccgct
    25261 ggatcggggg agtggcatcc cactcatcgc gttttttcca cgaataaatc gtgttctggt
    25321 ttattcccat cagctgagcg atatccgccg gggtataccc ctgccagtaa agctgtttcg
    25381 cccgcatacg aataaagaca tcctggatca ttgcaccccc tgacattaac agggcaagat
    25441 taagcctcag caataccgcc tgataccagc ccgcgttgtc cccgtcgcca tacaacttaa
    25501 gcaccttgag acacaccgtt atctgccgcc atcatgggaa aaacgaccag gttatggaga
    25561 atttccatgg gaagcaaaaa cacgccagag cgcaaaaaat ttcgcgttgc cgtttccggt
    25621 tcaaccatcg acggacgtga aatttctggt gaaatgctgc gtcaggccgc agaaaactat
    25681 gacccgtccg tttacgcctc ccgggtgaac attgagcacg tcctttcgat gtggccatcc
    25741 agtgaactct gcgcagtggg tgatctgatc gctctgtcaa ctgatgaaat caccgaaggg
    25801 aaactggcag ggcgtacagc gttatatgcc gaaattgaac ccaccgaccg catgaaaaac
    25861 atgattgctg acgggaaaaa agtgtattcg agcattgaga tgttgcctga gctggacgcc
    25921 gtcaaggggc cttacattgt gggtctggca atgaccgaca cgccagcaag tctgggcact
    25981 gaacgcctgg agtttgccgc acagcaacgc gccagcatca tgcagttcag taagtgcaca
    26041 acagaacccc ccatgttcac cgtcgccatg gaagcagaga tgcttaccac cacagaacag
    26101 cgaacggaag agagccgtca gtggttcagc cgcgtcatgg cactcatcag caaaacacgc
    26161 acagcggatg gcgagcagtt cagccagctg cgcgacgttg tggaaaacat cgcatccgat
    26221 catgcagatc ttatcgaccg tttcagcact ctggagcaga aacatgccct cgacaaaacg
    26281 gcgctggatg agctcagtct cgagcttgca gcactgaaag cgcaactgag aaaccaggat
    26341 gccgatccac agcagcgttt tacttccgct ggcggcactg accagacact ggctgaattc
    26401 tgacaggaga agagtaattt atggcccgtt tatccctttc ccgtgaagga cgtgacagtc
    26461 tggaacgcta tatgattcag caggcatcgc tggctggtat cacaacagag cgcctgggta
    26521 aaaccttcag cgtggatcca tccattcagc agaagatgga gaacgccatc aaggaaagcg
    26581 ccggactgtt acaggaaatc aattttatcg gtgtggatga ccaggaaggt gaaaaagtcc
    26641 tgattgacac cagcggcccc attgccagca cgaacgcaac gagcgacgga gtgaaacgcc
    26701 gcaatcctgc atcagttgct gatctggatg cacgccgtta ccgctgcgag caggtgaact
    26761 acgatacctt catcagctac gcacagattg acgcatggag cagccagaaa aactttcagc
    26821 aactgctgag cgcgcaaatc acccgccagg tagccctgga tcgaatcatg attggtttca
    26881 acggggagtc acacgcgctg atttccgatc gtgccaccaa ccctctcctg caggacgtca
    26941 acccgggctg gctgaaacac atccgcgaca aggctgccac ccgcgtggtt aaagggatga
    27001 ccctgacccg tcgcgatgat gaaaacaaac tggtggcaaa aggtgactat ggtaacccgg
    27061 atgccatggt gcacgacatc cgctcatccg tgctggatga atggcataag gacgcaccgg
    27121 atctggtcgt actgatgggg cgggatctgt tcaatacact gcgtcttccc atgattaacg
    27181 ccatcagcac caccaacccg aacactgagc ttgttgccgg gcagctgatt gtggcgtccc
    27241 gtaccgtcgg tggtcttcag acgtatcttg cgccgtactt cccgaaagac gccatgctca
    27301 tcacgtcgct gaaaaacctg tcgatctact tccagaaagg ctcactgcgc cgtttcattc
    27361 gcgaggagcc ggaatacaac cgtatcgcga cttaccagtc catgaatgac tgctacgtgg
    27421 tggaagacta cggaaaatgc gcgctgattg aggggatcac cttcgcaggt gaagatccgg
    27481 acaacaacgc cgaaagcgga cagccgcaac ccgaagcata acccggacgg gcgcgccgcg
    27541 cccgttaaca ggagctgaga aatgctgaca ccggcacaac gctatgtgca acaggcggtg
    27601 gcccgcaacc gggtgaccat gaacgcccgg gaaacacaac gggaaagaac cgcacatgaa
    27661 caggtccttc accagttaag gctggcacag atgcagctga agggtattca gtcaaacgtc
    27721 gccagggcag agcttaaaaa acatctgttg cctgactttg aagggtggat tgaaggcacc
    27781 ctggcaggag acagcggacg ccaggatgag gtcatcacca ccatgatggt gtgggccatt
    27841 gactgccgtg actacccgct ggcactgcgt atcggggagt acgtgatccg ccatggtctg
    27901 gcgctgccgg ataactttgg ccgtgacgcc gccacggtac tggctgaaga gattactgac
    27961 gccgttctga cgcaggccac aacggacagc gaggcggatt tgtctgtgta caccgccatg
    28021 cttgatgagc tgaaccacca ggtcagtgag caggatatgc cggaccaggt caggtcaaaa
    28081 ctgcacaagg cacgcgcatt ttcccgccgc gcctcctcct ctcccgatga tctgcatctg
    28141 tcgctgaacc tgttacgtga agccatgcgg atcaatccgg cttcgggagt gaagcgtgaa
    28201 attgccacgc tggagcgcgc ggtcaggaaa ctggcagacg gaaatcaggt ggtgcctgca
    28261 tccacaacgg gcaggaaaac caccaccggc gggaaaaaga gcagcgcaaa aaagaaaagc
    28321 gctgccagcc agaaaaaagc cgcgcgcaaa acaaactgat ttaacgaacc gcacctcgtc
    28381 ggcaggcggc gcaaccggtg atctgaacag gcttcgcgtt ctttttaccg gttgcccacc
    28441 gcctgatttt tacggagaca ggcatgagtc tgatagccag accccgcgtg aacagcgctg
    28501 acacagatac caccgacacc gatgacggcg acgccatggt gagcgcgggt gacttctggc
    28561 cggcgatctc actgcatgaa attcgcctgg cttcccgcat tccggggagg acaacaacaa
    28621 cccgtctgtt acatgcagca acggaagcgg tcgcccatgt gatcgccgaa ctggagctct
    28681 ggaaacgtga gcggatggct gaaggcgcgg cctcactggc tgacgttccg gccacgcagg
    28741 tgaacggaga gagtgtacac gtccaccgct accgcagggc cgttttcgca ctcacccgcg
    28801 cgtttgtact ggagcgcacc cgggatgtgg ataccaccga taaaggcgac cgcagggctg
    28861 acgcgctgga catgcaggtt gaggatttgt ggcgggatgc ccgctgggca atatcagaca
    28921 tacgcggtgt cacccgcatt tatgcggagc tggtctgatg aaagtaatgg ctctggacgg
    28981 tgacaccgtc gatttactct gtcagcgcca ttacggcacc acgcaggggg tgactgaggc
    29041 ggtactggcc gccaatccgg ggatttcagc cgtcatgttt ctgcatgccg gacaggaagt
    29101 ggagctgccg gagctgcagg ggcagacccg gcgtgaaatg atccagttgt gggggtgaga
    29161 atgtttcagc ggctttgtga tcacatcacg ttctgggcgt cagtgacgac agccgggatc
    29221 ggcgtgatga cgctgagcga aaaaattgcc gtggccggtt ttgtgctggg tacgctgtca
    29281 ctgcttcgtg cctggcttca ccgccgcagg atggagatcg cccaggcaag gcgtaacgaa
    29341 ctgattgaaa aaatccttat gcagtcagaa ggaagggagc tgaacgacgc agagcgccgg
    29401 gcaatgatgc ttctgaagca ggatgagcac gatggccgga cctaagcgtt acgccgtggc
    29461 cgccattgtg gcgctgggga tctccctggc tcccggagaa ctgagaacct cccgcgaggc
    29521 acaaatcaaa attgccacat gggaggagtg ccgggcccga ccataccgtg atctggtggg
    29581 ggtcggtacg gtcggatgcg gatccaccgg taacgtggaa aaccgcctgt acacacagga
    29641 ggaagtggcc gggcggtgga tcaacgatat gcgccgcgct gagaactgca tcacacagaa
    29701 tttcaggggg cagcagatgc cgcagtctgc attcgaagcc atgacggacg ccgcctttaa
    29761 cctgggctgc cggaatctga tgtggtttaa aaacaaaaac ggcacgccgc aacgcaccac
    29821 catctggaaa cacgcgcaga cacggcaatg gcgcctcatg tgttaccggc tgacagattt
    29881 tgtgaacagt ggcggaacgc gcacgcaggg actggttaac cgccggaatg attttaagaa
    29941 ctggtgcctg aaagacgcgg gagcgcagcc atgaaactga acattatcct ttgtgcgctg
    30001 ctgctgacca tttcgctgca ggcaatggtg gtcatccggg cctatcgctc aggtcaggag
    30061 agcacccgtt atgaagcgct ggaaaaagcc gtgaaagaga gcaatacgac cgtccgggag
    30121 ttgcttaagg ccacggcgga gacacagtca gcgctggccg ctatccggcg cgaagatcag
    30181 gagcgtgcag ccagagggga agaacgacgt gaaacgatgc ggaaaaactt acaggcggat
    30241 gagtgcgcac gcacttctcc ccctgctgat gttgctgaca gcctgcaaca acacgccagc
    30301 gccgctgaaa accggcgtca tttgcgggag cgtgccggaa agtctgacca gacagacgcc
    30361 ggtaccggtg ctgccgcaac acatgatatg ggctgatgtg gctgactggg gagaccgact
    30421 ggtagatgcc ctggacagtt gtaatgccga taaagccgcc attgccgggc tggagcggca
    30481 gcggacagca cgaatgaaca ctcatgaaaa ataacggagt cactatggta ccgaaacatt
    30541 atcagcttgt gacggcaatg acgaacaagg aacttgccga aaaacttgcg cccatgctca
    30601 gggaaggatg gggacttctt gggggacctg tctcagcatc agagccggga gcaccatttt
    30661 acctgatgca ggcactggtc actgacagag acctgccatc agccattccg gtaccagaag
    30721 gcgaggaaaa acccgtaccg ggcactaccg acggatacaa cactggacac acaactgagc
    30781 cagagtttta ttatgtggtg ccactggctg gtcagtccaa tggcatggca tacggtgagg
    30841 ggctgccatt accggataca ttcgacagtc ctgatccacg aattaaacag ttagcgcgtc
    30901 gcagtacggt gacaccgggc ggcgctgcat gtacctataa cgatattatt ccggcagatc
    30961 actgtctcca tgatgtacag gatatgagca gccttaatca tccgaacgcc gatctgtcaa
    31021 aaggtcagta tggctgcgtc ggacagggtt tacatattgc caaaaaatta ctggcgttta
    31081 tcccgaaaaa tgcagggatc ctcctggtac cttgctgtcg tggagggtca gcgtttactt
    31141 ccggcgcaga gggggcattc agtgaatctt caggagcaac ggccgaatca tccctgtggg
    31201 gcgtcggcaa accgctgtat aacgatctgc ttacccgtac aaaagccgcg ctggataaaa
    31261 accccaaaaa cgtcctgtta tccgtggtct ggatgctggg agagtttgat ctgaaatcag
    31321 caacatacgc acagtacccg caattattcc tgaacatggt gaatcagttc cgcgcagatc
    31381 tcagcgcata tgctgcacag tgcaccggag gcaacgcatc acttgttccc tggatttgcg
    31441 gcgataccac ctatatctgg aaacaacaat atacctctca gtacccggtg gtttacggtg
    31501 catatcagaa caaagaagcg caaaaaattt attttgtgcc cctgatgacc gatgagaaag
    31561 gtgctaacac gccaacaaac ctgcctgccg aagatcctga cctggcagaa gccggttatt
    31621 acggtgctgc atcccgtagt aacggaaact ggacctcttc agatcgcgcc acgcatttca
    31681 gttcatgggc ccgccgcggc attatttctg accgcctggc aacggcgatc ctgatgcatg
    31741 ccgggaagac cgtcgccttc ctgaaaggtg cacccgcacc gttcagcgtg ctcgcagtac
    31801 ctcccgccac ggccggttca gacagcacca ccacggaaag cccgcagccc tccacacctg
    31861 cacctcagcc gggcgcaggt acgcagtcat caccggcaag aacaacggcc gtcgcttatc
    31921 gcgcagacgc acaggaaggg aaactgaaag aacagggctg gacaggggca gatggaaaag
    31981 cctccgtcgt aaatgactct gctgctgaga gcggtaaagc catgcgagtg gaaaaagtcc
    32041 aggggcaaca aacagcctgg aaaatcgccc acgatgtgcc tgcaagtaaa gcagctgaat
    32101 tacttcagaa aggcggggaa attgcctttc gtttcaaaat cccggaaggt gttcagctgg
    32161 cagaaaaccg cttcgccatg gctctgcact ggccggtttc tcagctacct gaaaacgttg
    32221 ttctggaagg cagcgcagga aacgatatgc tggccgcctt cttcatccag agcgacagcg
    32281 caaacctgaa tctgatgcag cacaaacaac agaatgcaaa actgggaacc ttcggggcat
    32341 ttaatcataa ctggcacaac gtggtgttcc ggtttgccgg aaataacagc atcagcgtga
    32401 caccggtcat caacggtcag acccaggcgg cctttaatct ggcgaaatgc ccggcggcag
    32461 gactggataa cgacaaactg ctgatcacgg atatcaccag taaccaggac acctaccccg
    32521 tgctgattga gtatgtgacg gtggatatca ataacagcaa cgccgcataa gaaaaaagag
    32581 gctcccggca ggcaagactg agaggaaaag atacctgacg ggagcgcaag aggaattatg
    32641 gagagtgttt attattgtta ttcatttttt ttaaaacgcc agacatcgtt atgtcaatca
    32701 tttgtgtgag gtgattatgt tattcattca tcagatcatg ctgtatttct gtactgccgt
    32761 ctgcgcgctg tatctgatca gcggcggtta cagtgttatc agaaattatt ttcagcgaaa
    32821 aattgacgag cgcgccgctg aaaaaatcag tcagaagtct gcacaacaga acaaccagaa
    32881 catgccataa cacacaggag ggggcatgct gaaaattaac accctgcgcg acgccatgac
    32941 tgacgcctgc ccatggtgtc gcgccaaccc ggagaaattc actgtgtatg tgaataacgg
    33001 aggcattgag acgaccggcg aatccccctc atatctttac cgttacacgc ttgggatctt
    33061 tgtcatggac ttcacgcagg atctcgatga actgatggtg ccgctgatgc gctggctgta
    33121 ccgcaatcag cctgatctcc tgcttaatcc ggagaaaaac agggatatca aattttctgt
    33181 tgccatcaat gacgacgaca gcaccgatat cctgctggaa atcccggtat gggagcgggt
    33241 gattatccgc cgtaatgatg atggcactgt tcacgcagaa cacatcggag agccgcctgt
    33301 cagccgggcc ctggaagcat ggcgcgtcac cctcgaagat gtcacatggg gtcagaatga
    33361 tggctccgga tgacctgcac agactggacg gtctctttca ggagatcctc agtggtttgt
    33421 ccgctgccgg tataaacaaa acagcccgtg ctgtcggtca ggctgtccgc gccgcgcaac
    33481 aaaagcgtat acgttcgcaa caatcaccgg acggtaaccg gtggcccgcc cgtaaaaaac
    33541 gtgtattccg tgtccaggca ggtgtggtgt tcatctggaa cggcactcag gtgagacgac
    33601 tgaaaaactg gcggacaaca accggccggt acggccccat gatcaccggt tacgacacgg
    33661 agcgccgggg aatacgctcg ttccacaaag ccgatattga agaatacctg gaaattgaca
    33721 cccgcaggcg aacgcagagc gccattaagc gtccacccat gtttgagcag cttcggacaa
    33781 accgctttct gaaggtaaaa accgatcccg gcggcgtcag cgtgggattt gaaggccgcg
    33841 cagcccgcat tgcccgcgtt catcagttcg gcgaagtgtc actggtctct gccggtaatg
    33901 ccgtccgcta tccgcaacgc gaactgctgg gattcagtga agccgacaga cagaaagtga
    33961 cggaaatcat tatcaacaac ctgtggagaa acacccgttg aatgctgaaa tcatgcgact
    34021 gctggaaaac atggtccgca ccggcgtcgt ttcggaggtg gatctgaaaa actggcgcat
    34081 tcgcgctacc agcggcggac tgacaacgga ctggctccgc tggaactgca cacgcgccgg
    34141 ggcgtttaaa atctggctgc ccccctcccc cggtgaacag gtgctgttgc tctgcccggg
    34201 cggcagtcct gaaatggccg ttgtggctgg cagtctctac agcgaagaac acccggcgcc
    34261 cggcacaaaa cagaatgaag tgatactcct tgcacctgac ggtgccgaga tacgctatga
    34321 tgcagaaaaa ggcgcgctga cggtgaaagg aatcaaaacc gcagagatct ctgcggaaac
    34381 acgtattacc ctggacacac cggccgtaga gtgcacaaaa catctgaagg tcaggacgtt
    34441 tgaactgact gacggtggca ccctgaaagg ggatatcaca cacagtaacg gaaacttact
    34501 ctcaaatggt gttaccgtgc atacgcacgt tcataacggt gttcagtccg gcggaagcat
    34561 gacagggggg ccgaaatgac accggtatgc acaggcatga acccgcatgg tagcggatgc
    34621 atcagtgaca cagaacacct ccgccactct gtaaacaata ttctgaccac cccgcgcggc
    34681 agtcgcatca tgcgaagaga gtatggcagc ctgttacctg aactgctcga tgcgccacaa
    34741 aacgacgtga cgcgtctgca gtgcatgagc gcggtggtta tggcgctcgc acagtgggag
    34801 ccccggatcg ccattaaccg ggtggatatc gcctggctgc ctgacggacg cgctctgctg
    34861 gccatctcag gcgtgctcac ccgcacaatg cagcccgtac agatgagcat tccgctgagg
    34921 gggagtaatg ccgacaattg acctgtctca gttaccacgt ccgcagatca ttgaatcact
    34981 ggattatgag agcatactga aagacgtgaa ggcctttatg attgatgcct tccctgaaaa
    35041 catgcgcagt gccgtcagcg cagccattgc gctggaatca gagccactca acatcattgc
    35101 tcaggccatc gcctttcgcg aaatgctgct ccgtcagcgg atcaacgatg cagcggcggg
    35161 gtgcatgctc agccatgccg tatccacaga cctggataat ctcgccgcta tcgtgaatgc
    35221 gaaacgcctt gaactgaaac ctgctactga tacagaagct gccatcatgg agagcgacag
    35281 cgcgttccgt ctgcgggcac aatccggatt tgaaggtttc agcgtcgcag gcccctctgc
    35341 tgcatatgaa tattttgccc gcagtgccag cggtcttgtt gccgatgcac gggcaaccag
    35401 tccgtcacct gcgacggtca ttgtctccat tctctcaacg gaaggagacg gcacagcgtc
    35461 accggaatta atcgcgaccg tcagccgggc tctcaatgat gaaactgtgc gccccctggc
    35521 cgacagactc acggtacaga gtgcgaccat cgtcagttac gatattgagg ccgtactcta
    35581 tttttatccg ggcccggaat ccgaacccat tcttgccgcc gccagaaatg cacttgacac
    35641 atggctgaga actcagaatg ccataggacg tgatgtggcc cgctctgcca ttatggcaac
    35701 gctacacgta ccgggcgttc agcgagtcga gctgaagaaa ccagcgcaga atgtggtgat
    35761 cagtaacacc cagtcagccc gctgcacatc ccggaacatc tctgcaggtg gcaccgatga
    35821 ataaccgact gctgccgcca tcatcgccct ccttcctgca tcattcggaa cagggcgctc
    35881 agcgcctgtc agagctgccg atccatatca acaccctctg gaaccccgac acctgcccag
    35941 cccatctgct gccatggctt gcctgggcgc tttccgttga ccgctgggat gaaacatgga
    36001 gtgaagaggc acgtcgtcag atcatccgtg actcgtggca tattcaccgc cacaaaggca
    36061 ccatcagcgc catgcgtcgg gctgtggagc ccttcggcgc gatactgagt attacagaat
    36121 ggtgggagaa caacgatccg ccggggtcat tcaggctgga tattgacata tcggagcgcg
    36181 gactgacaga ggaaacccgg caggagatgg agcgaatgat taccaccgtt cgcccggtaa
    36241 gccgacacct tgccgccatg aaaatgacct acgttgcacg gggtgtatcc cgcataaccg
    36301 gggccacgct gaccggggaa accatcgtgg tatatccggc agcagaactg acaagcccgc
    36361 tgtcagtcag tggtgacatc cacccgggtg gagtcaccta tgaaggcatc acagtaacca
    36421 tcagaccgga caaggagtaa tgatggccgc aaaatacaaa accattatca caaatgccgg
    36481 cacagcagcc ctcgcagctg cggtgttacc ggatggagaa aagatcacgc tgacacatat
    36541 cgccgtcgga gacggtaacg gtaaacccac agaaccactg gccacgcaaa ccgctctcgt
    36601 taaagaggtc tggcggggcg aggtcagcag ggtcagccgt gacgcggaaa acaccagttt
    36661 tttaaccgcc gaagtgatca ttcctgcgaa tgaaggagga ttctggatcc gcgaaatcgg
    36721 tctgtttgac agcaaaggca cactcatcgc cgtgggtaat gttgcggaag gttacaaacc
    36781 gcagcttgat gagggctcag gcagagaaca ggtattccgc atggtgattc tgctgagcaa
    36841 cgaagcctct gtaaatctga cagccgactc atcaaccacc atggcaacac aggctggcgt
    36901 ggcagaaatg ataaagcagc atgaacaaaa cggtaaacac ccggatgcca caaccaccag
    36961 taaaggattt actcagctca gtagtgaaac cgacagtgat gatgaaaccc gggcagccac
    37021 acccaaagca ataaaagcac ttaaggcgct gacagtaaca agaccaacag cccccctcgc
    37081 cgtggatctg aatacactgg ggagacctga acatcatggt gaccactatc agcccaacaa
    37141 caacggcgcc actacagcac atcactaccc ggtaacatca gccggatcat tatccatcag
    37201 gatgggcgca tggggattct gccagcagga atacaccgcc tggaatccac cacgaaaatt
    37261 tatccggaca gtgacaggta acttcaccgg caacgggcca tggtctgaat ggaaggaggt
    37321 tgccatactc cccctcacgc agaccggcga cgcctccctg acagagcgag gtattgttca
    37381 gctaagcagc gcaacggaca gtgacagtga agtgctggct gccacaccaa aagctgtgaa
    37441 gacggccatg acggcagcag aaaacgccgg gcagaaagca cggcaagcac tggaggctgc
    37501 acaggcaaaa acacagcgga cactctggga tgtgggcagc tttattctgg cggcaacgga
    37561 tgccgaaggg gagtggccag ccaaaattga tataacggcc ggaaacaccc gcccgggcag
    37621 taaactcaga ctgataagcc tgactcattc cgaaggcaac ttcggccccc gtgaatccat
    37681 gcaaaactgg aacacaaacc gcaggccacc gggcacgtgg cgggcctgcg ctgacattta
    37741 tgcaggcgca ggaatcggaa tatttgagcg cattgcctga cggagagact catgcacaca
    37801 atcaaatcag caagaaatgc cgcttataac cgtgacggca ctatcgactg tgagatccag
    37861 tttgaagggg aggagagctg gctgccctat acggcaaccg aatatgacaa ctcgccacac
    37921 ggcaaaaaac tctggggcga actgaaagca ggaaatcacg gccctgtatc tgaataccag
    37981 gaaaacgaca ctgacaggga aaacgccaga cgcctgaaaa acgacgaaat caacacctgg
    38041 cgtaatgcga tggaagcggc gagttacacc tttgaacata acggccggaa atgggactac
    38101 gggaagtcaa cgcaggcccg ccttgagcct tccgttgctg ccgcaaaagc ggggatcctg
    38161 ccgctggatt ttttctggac tgatgcacag aacaacgatg ttcctgtttc tgctgatgag
    38221 ctgattgccc tcagcgatgc agccgcaaaa gccctgtttg ccaaagggat ggaaattcac
    38281 attcgccagc gtaacatgaa aaaagagatc gccgccctga acgatacggc ggcgattctg
    38341 gactataagg ttggctgggg aaaagaagcc gaagcctgaa cagtgacctt cacaacatga
    38401 gaccagacag cgcagaggac gccgacgaca gggccgacga cgcgctgttt tttaatcctg
    38461 ccatcagatc acccaccgag cccgactgaa gacgctcacg cagatcttca ttgcatcgct
    38521 gaaagctgac agtgaactca attttgcggg ccatgccata gcgatccagc tcctgacggg
    38581 tggtcctgat ttccgtaatg acatacatcc cgtaaatctg tccgacacca tcaatcagtg
    38641 gccagggtaa tcctgtgtag gccagggtgt tcagggcagt cagtgacacc tccccgccgg
    38701 tgatttccgg acagagcaca ccggacatcg tgatgtgatc ttcaccagag ccaatatact
    38761 gccaggagga tgaacggtta acccgttcat tactgacgtg tcgccagcgt ttactctgtt
    38821 ccacctgctg atgcggcagg gtacttaact gaaacacaaa catcccataa atcatcatca
    38881 tctttttcac ctcagtcgcg atcgcggaag ctgccggttc cggcacgtgc cattctgttc
    38941 atttccgccc tgacagcgtc accaaccatg cgcgccagtt cccggggatt atgcgaactc
    39001 accccgtgaa gatgcacatg tatctccccc tgaaaggcgc tggcgaaggt ttcctgctgg
    39061 tgcattcgtg gcgccgggcg tgccgtcggc cattgctgca cctgaatctc ctcctgtggc
    39121 agcgcgcgca ttccgccgga agacggcgca acgacaggtt catgccactg cccccggacc
    39181 gccagcgccc gtggaagatg tttaaacaca atatctccgg gtccgatacg tttacggttt
    39241 tcagccagca gaccaccggt attcccctca atgcctttca ggtgacggag aacgttacgg
    39301 ttaccgtccg gcgctatccc cgcagggagc gccaccggcg gaggctgtgt accggaggcg
    39361 ccggaaggca cactggcccc accaggaggc gaccagtgcc acgccttctg aaccattttt
    39421 ttctgttccg gatcccactc ccacgccacc ggcgttttac gggcctcatc cagttttcgc
    39481 ttaacccgct cagcttcacc gggcagaaca ccgagttttt ccagcaccca cgccagaccg
    39541 tcaatcagag ctttcagggg cgaaaaaacc gtggctaacg caccggccag agtactcccg
    39601 aacatctgcc cggcagaggt gcactgctca agcaaatcac gcgtggagga gaccggcgtt
    39661 aacagggatt taaaccatcc cagaatacgg gacagtgccc ctgaaaccag atccagcagc
    39721 ggcgcaaaac gggaggctgt cgccatgaag ggcgccagtg ctgactgtac cccttcaata
    39781 aatccgctca caaacgccct gacccgatcc cagtagcgcc agaccagcag agcaccaccg
    39841 accagggctc ccaccgccac cacaaccggc caggtcagcg ccccaagcgc agcccccacg
    39901 gctgaaatca cacccgtcag tccgccaaac agcgtaccgg caacggagag tccggccggg
    39961 atcgcgcgaa gcagggcaag ccccagacgc agtttcagta acggtgcgac aaccatccag
    40021 gccgcaacgg acaacgcccc catcgctgtg gttaacgcca gcaccgctcc cgtcgtcagc
    40081 gcaatcattc gcaccagttc cggatgcgca ttcacccacg cctgcagacg ctcaccccac
    40141 gctgtggcca tccggacaag cccacgcaga tcgtccgtaa tctccccgat aatcccactt
    40201 ctcagcgcgc tgaacgtccc ggtaagccgc gccacatcac cactcagatt gtcacgcagg
    40261 gtttgcccca tccggtccgc cacaccagac acgtcagcca tacggtcaga cacccgcgcc
    40321 agcgcgccaa gaaaggcagg gatttgatcg acagacagat cttcaaccgg tgtaccaaaa
    40381 agtgcgatgg ccgcattggc ccgctcagcc ggatcgacga tctgcaacag acccgaggct
    40441 gttttctgca gcgcagcccg tgcagccgtt ccaccactgg ctacagccga tgacatcctg
    40501 gctgcatcca gcccgataag cgcatacgct tcagcactcg cctttgacat atcggagccg
    40561 cgaatactga actccttgat ggcatcgccg gttttatcca gcgcaaactt cccctgctgc
    40621 gccatatccg caaggagcgt catggtttcc gaccctgtca gccccatgtt acgaaaatgg
    40681 gtcgaatatt cgtgaaggat ctcaggcagc tcatcgcgca taaccggccc catgcgctgc
    40741 atgcccgcca ccatcagatc aaccgcctcg tcactgctgc gtgcaagacc gtttttgatc
    40801 atgatggcag ccgcctgaat gtgctgcggg atatcgcccc ccatcacggc ctgcatatcc
    40861 agcatttttc gggtgattcg ggtcagttca ttctcactaa ccgcccccag cccacccagc
    40921 gtggagcgga cagcacccac agcttcagcc gccagttgtg catccggtgt gataccggta
    40981 tgaaccatgt ggtttataac cccggtatac cccacggcat ctgacgcgcc ctctcctgcc
    41041 tgagccgcaa tgacggcacc atgacgctgg agatccatca ccggcgcaac ggcacgcccg
    41101 ccagcgtaaa gtcctgctgc accggcagcc agtgccgccg caccggcatt acgtatccgc
    41161 ccgagtcgct cctgcgcctg ttcatagcgt tgctgtgcac gggtcacctg tgcgagtgcc
    41221 tgccgctgtc gcgccagcgc ttcgttatac tctgccgtcc ggcgaatggc tgcctgtatg
    41281 gtctgactgc tgccctctgc ggcaataccg tgtctctcca gctcccggcg tgaatcctcc
    41341 aggcgtcgct gatgtacatc cctcacccgg ttcagacgtt caagccgggt ggtcagcgaa
    41401 gccatcattt cacgctgccg ttcactgagc acggtaccct cacgctccgc ctgctgcaga
    41461 cggttcagtt gctgctgaac agcggcaatt ttccgggtgg cttcgtcaaa tttagtctgt
    41521 gccgcagcaa aggcttcact ctgccgttca agcgagcgga tggagtcctg agtttcacgg
    41581 agggaatcag aaagcgcgcc cacggtttcg cgggcgcgcc tgagaggttg tgtcagggtg
    41641 tctgccgccg ccagagcaac cctgatatgc agattattca tcatgattgt gacttccgct
    41701 tctggcagcc gcccgctcac gccaggcgac cacctctgca ggcgtcattg caaatatctc
    41761 tgcgggcggc cagttaaaaa taacagcaat atccgcaacg ggatcttcaa tgcagtcaaa
    41821 gcaaagacag gttacggctc tgccgtgttc tccggtgccg gtgcattcga ccccgagggt
    41881 gcgaaaaaat taaccgccgc cgagctcagc gccgtaaaat ccgccgtatt catggcatac
    41941 atttcggcct ccttgagttt tggctctgtc acacgcgcaa gccaggtgac catggcatca
    42001 cagttcatgt tgtaaagcgc aagcaaagac agcccacgca gcgatccggc ctgattcgcc
    42061 agcggtccga ttttaatttc actgtacgac acatctccgc gcacaacagg caccgtcagc
    42121 ttcaccacgt tcttaggtgc agccgtagcg gcaactgcca ccgcggaagc ctccccgtta
    42181 ctgacggcag tgccagtggc catttccgtt tctttatcca ttttcagtct ccacggcggg
    42241 atccccgccg ttctctgtta aaccatcatc agaagcccag catcgagcgg gtgcgatccg
    42301 gcagcacatc cttaccgtca atgcgccaga taaaattcag cacatcaaat tcgatttttt
    42361 ccagatcgtc cacactcagt ctgtaccagg tgtttttcat cgaataactg tgctcagtgt
    42421 cttcaccctg cttcgcctcc cccatatcga tttcagtgat gcgcccgcgg acctcaatat
    42481 ccacatatct gatatccgca tccgaatagt actcaccgga aaagcgaaga cggacttcat
    42541 caagtgaccc ggcataacgg gagaaaaggt gggcatctgc gccgcctacc gcaaaagaga
    42601 gatccagtgc accatcatca agaccaaagt cgacggaaac actgccgggc atcccggcac
    42661 cacggaagga ctccgttttt cgggtcagtt taggcggcgt gaaggacgtc acccgcccca
    42721 gcttgttttc gccatcgatg tacaccgtga aaacgcgaag tttgtttggt accgccatta
    42781 ctcacctccc agtgatgcaa atgcaccgtc aaagaactca tcagtaaacg tctggtgaag
    42841 ctccagatct tcaagcggtg gtaccggcgt atatctgtag cggatccgca caatcccctg
    42901 acgcagatcc gttgtgccgt tatccaccac gtcataccag cactctgccc cgataagttt
    42961 acccgctgtc acaagggcac tcagttttgc ccggatatcg cttaacacat ccttcacgtt
    43021 cgacggcgtc agcggcttgt ccacggtttc aaactgcgat tcggcgatgg tatccgccag
    43081 cgactgcgcc gtacgcgtgt aggtctcaaa ggtgtaggta gtggtgtcga gagtccggtt
    43141 accccagaaa cggaagccgt tacgtttaat gagtgtggtg atctccttgt cattgagggt
    43201 ttccgcgtca ctgtcctctg cctggagcga ccagaacacc ggcacagaaa tacccaggac
    43261 attattcact gccacattgg acagggattt atgccagccc tgctcgtggt caatcttcgc
    43321 acgcaacccg cacgcatacg cagatgcagg aaaagcctca ttctggccac tgttagggtt
    43381 gtacgcgata aaatcaggcc agatcagcat aacttcacgg tgagcaagat cggcgcgcca
    43441 ggtaacagcc tcctgcacgg tattacagcc gggacagccc gcatacacaa aggcacgcag
    43501 ctttttcgcg acggttacca gtgccgccac cacctctttg gtgtcaagcg atggcacggc
    43561 caggatcctg ggacgaacac caacagcctc attctgttca gccaccagga gcgcctgcat
    43621 gcctgtatag gttccgtcat ttttgacctc accaataaca agctgcgttt gtgtcggctg
    43681 accatccccc tccttcgcag cagcaacccg caccacaaca actctggtgc tgacctgatc
    43741 ggcaatagct ttcagacttt tgtacagcgt gccggttttc ccggccttac ccagcacatc
    43801 cataacgcgc gtcaggagca ccggcttatc cagcggaaat gtttttgcat cagcatcatc
    43861 agcaacagcc acgataccaa tcacactgga gtcaatatca ttaatggccg tgaccagatc
    43921 cgcattctcc cggacccgca cgccatggaa acgggtttca gccatattat tcaccctgca
    43981 ttcgtgtgag ttcagcagag atctttacga acacggggag ctttttcacg aactgcggtt
    44041 tctcgcaata cacatacaac ccaaactcat tccggtggtg tttctggtat gcaactgtgg
    44101 cgggaaaaac ggaggaatta tggtattgct gaacggtaac acggtttatt cccccctgaa
    44161 agaactggtg aaaatgcctg atttcagcgt ttcactggac agggagcagg ataaaaacac
    44221 tcttcaggaa ctgaataacc gggtgatatc gctttccatg acagacaacc gggggtttga
    44281 agcagacagg gtcactcttg tgctggatga tgccgacggg cagctggctc tccccgggcg
    44341 gggaacacac atcagagtca tgctgggatg gaagggagag cctctggttg ataaaggcac
    44401 ctacattgtt gatgaggtcg ctcatgaagg acctccggat cggctgacac tgagtgcaaa
    44461 cagcgcagac ttcagggagg aatttaacgt caaacgggaa gtatcctggc acgacgtgac
    44521 tgttgaacgc gtggtttccg ctattgccca ccgttacgga ctgaaagccc agataagcga
    44581 tatgcttatg gatattgaaa tcgatcatgc cgaccagacg gaagaaagcg acatgtcatt
    44641 ccttacccgc atggctgata tgctgggagc catcactacc gtcaaaaatg gctgtctgct
    44701 gttcattctg ccgggtggcg gcgtgacagc tgacggacgc ccactcccct catttgcact
    44761 ggcacgcagt gacggagaca gtcaccagtt cagaatagcc gatcgccagg catacacagg
    44821 ggtgaaggcg tactggctgg atttgaatca tggcaaaaag aaagccgtca acataaaaaa
    44881 acgaaaaact cacgctgaaa agagcagcag ccgggaaggc agctacatgg agggggcaga
    44941 aggtaacgtg ttcgttctga gaaaaaccta cccaaatgaa attgctgcac agcgcgcagc
    45001 tgccgccagg tggcagcaat tacagcgcgg tgcggctgaa ttctccatca cgctggcccg
    45061 gggcagagcg gagctttacc cggaaatgca tggcacggta accggcttta aaaccgagat
    45121 agacaatcag gactggctca ttgcaaaagt ggaacacaaa atcgacggaa gcggcttcac
    45181 cacaaggctt gaactggagg caagaatacc cgagtggata gctgaaaagg aaagcaatgg
    45241 ttagaatggc gacgagttca accccagttc agccaggggg gtatatcaat gtttcattgt
    45301 ccattctgcg gcgcatctgc ccggatccgt accagtaaag ccgccaatga taccggcacc
    45361 atccgctaca aatactatca gtgcaataac cttgagtgca gcgccacatt ttcaacaatg
    45421 gaaagtgtgc acagaattat tggccagcct cagttgctct tccatgataa agatattcca
    45481 cgggagacaa tgcgcaaatc ccatcaggga cgcagtcagt taacgctaca actggaggat
    45541 tgcggcatat cctcaaaacc agtacattac gcgcgggtgc ctttcggctg acggctggaa
    45601 ggaaaacctg aaggccggat gtggaaaggc cccgagtcaa acatattgtt taacccgagg
    45661 ccctaaccat tcctacccta gacaagtgga aggttagcgc ctccctgtaa aaggagtcaa
    45721 gcgctatgcc gcaaaaaacg atcatcgtga gcattttatg cttaaccatg ttgttggcca
    45781 tctggatgat acaccgttcg ccgtgtgagt tcagacttaa catcatgtgg tcagaatttg
    45841 cggcgttctt gcagtgtaaa cagtaagaaa ccgcagcggg ggtgaaagcc cccgctatcc
    45901 ggttgtcggg tgcagtcgat atggcacccc tttctccatt ggattacaga cgtatttgcc
    45961 cgcaatatgc gggctttttt gccgccctcc ccctaaacag tttcaacact gagcattgcg
    46021 cgcacaaagg gaaatcagta gattacacgt gggtgccttt cggctgacgg ctggaaggat
    46081 tacctgaagg ccggatgtgg aaaggccccg agtcaactta aacgttaacc cgaggcccta
    46141 accatctaac cttagcaagt gacaggttag cgcctctcca acaaaggagc aagcgctatg
    46201 tcgcaaaaat cgcttatggc catcacgttc tgcgtgacgg caatcctcat catctggatg
    46261 ttacatggtt cgctgtgtga aatacggatg aacatctggg gagcggagtt tgcggcgttc
    46321 ttacagtgta agcagtaagg aaaccgcgac gggggagtaa tcccccgtca atcggttgtc
    46381 agggtaaggc cgaaagtagc accctcacac gcaagtcaca aagaaaaata aagcccgcag
    46441 cgtaaaaact gcgggctttt tttgtttcaa agatgggcat tatatggaca cctgaaaata
    46501 aatccctttt attttcagtt aattaacctc atgcaaaaag cgcccgcagg tgcctttttt
    46561 ctgcgttacc tgttggccta cacagtaaag aaattacgcg gaagatgaag cgtaatcaga
    46621 cctcttccat gcgacccagc agggcctgca gacgttcctg ccagccgttc tgctgttctt
    46681 tcagatggtt gttctcacgc tccagctctt cgcgctgatg ctgggcattt tgaacttcct
    46741 gcgacagtga gttgtttttt tctttcagct cttcgatttc catctgcaac agagtgatgg
    46801 tatcaatcgc ctgctgtact tttgcttcca gtttctcaaa cacttctaat gacattgtca
    46861 tacctctcct gaattgcaag gcgttgatgg ataaaaatcc tcgtcccgat taccggtgac
    46921 gccttaataa atacgagcgc actttagtta gctccgattg tatgaagccg cgccatcgct
    46981 gtccagcggc acgccttgca gattacggtt tgccacactt ttcatccttc tcctggtgac
    47041 ataatccaca tcaatcgaaa atgttaataa atttgttgcg cgaatgatct aacaaacatg
    47101 catcatgtac aatcagatgg aataaatggc gcgataacgc tcattttatg acgaagcaca
    47161 cacattttaa gttcgatatt tctcgttttt gctcgttaac gataagttta cagcatgcct
    47221 acaagcatcg tggaggtccg tgactttcac gcatacaaca aacattaact cttcaggatc
    47281 cgattatgag tcaaacatca accttgaaag gccagtgcat tgctgaattc ctcggtaccg
    47341 ggttgttgat tttcttcggt gtgggttgcg ttgcagcact aaaagtcgct ggtgcgtctt
    47401 ttggtcagtg ggaaatcagt gtcatttggg gactgggggt ggcaatggcc atctacctga
    47461 ccgcaggggt ttccggcgcg catcttaatc ccgctgttac cattgcattg tggctgtttg
    47521 cctgtttcga caagcgcaaa gttattcctt ttatcgtttc acaagttgcc ggcgctttct
    47581 gtgctgcggc tttagtttac gggctttact acaatttatt tttcgacttc gagcagactc
    47641 atcacattgt tcgcggcagc gttgaaagtg ttgatctggc tggcactttc tctacttacc
    47701 ctaatcctca tatcaatttt gtgcaggctt tcgcagttga gatggtgatt accgctattc
    47761 tgatggggct gatcctggcg ttaacggacg atggcaacgg tgtaccacgc ggccctttgg
    47821 ctcccttgct gattggtcta ctgattgcgg tcattggcgc atctatgggc ccattgacag
    47881 gttttgccat gaacccagcg cgtgacttcg gtccgaaagt ctttgcctgg ctggcgggct
    47941 ggggcaatgt cgcctttacc ggcggcagag acattcctta cttcctggtg ccgcttttcg
    48001 gccctatcgt tggcgcgatt gtaggtgcat ttgcctaccg caaactgatt ggtcgccatt
    48061 tgccttgcga tatctgtgtt gtggaagaaa aggaaaccac aactccttca gaacaaaaag
    48121 cttcgctgta atatgactac gggacaatta aacatgactg aaaaaaaata tatcgttgcg
    48181 ctcgaccagg gcaccaccag ctcccgcgcg gtcgtaatgg atcacgatgc caatatcatt
    48241 agcgtgtcgc agcgcgaatt tgagcaaatc tacccaaaac caggttgggt agaacacgac
    48301 ccaatggaaa tctgggccac ccaaagctcc acgctggtag aagtgctggc gaaagccgat
    48361 atcagttccg atcaaattgc agctatcggt attacgaacc agcgtgaaac cactattgtc
    48421 tgggaaaaag aaaccggcaa gcctatctat aacgccattg tctggcagtg ccgtcgtacc
    48481 gcagaaatct gcgagcattt aaaacgtgac ggtttagaag attatatccg cagcaatacc
    48541 ggtctggtga ttgacccgta cttttctggc accaaagtga agtggatcct cgaccatgtg
    48601 gaaggctctc gcgagcgtgc acgtcgtggt gaattgctgt ttggtacggt tgatacgtgg
    48661 cttatctgga aaatgactca gggccgtgtc catgtgaccg attacaccaa cgcctctcgt
    48721 accatgttgt tcaacatcca taccctggac tgggacgaca aaatgctgga agtgctggat
    48781 attccgcgcg agatgctgcc agaagtgcgt cgttcttccg aagtatacgg tcagactaac
    48841 attggcggca aaggcggcac gcgtattcca atctccggga tcgccggtga ccagcaggcc
    48901 gcgctgtttg gtcagttgtg cgtgaaagaa gggatggcga agaacaccta tggcactggc
    48961 tgctttatgc tgatgaacac tggcgagaaa gcggtgaaat cagaaaacgg cctgctgacc
    49021 accatcgcct gcggcccgac tggcgaagtg aactatgcgt tggaaggtgc ggtgtttatg
    49081 gcaggcgcat ccattcagtg gctgcgcgat gagatgaagt tgattaacga cgcctacgat
    49141 tccgaatatt tcgccaccaa agtgcaaaac accaatggtg tgtatgtggt tccggcattt
    49201 accgggctgg gtgcgccgta ctgggacccg tatgcgcgcg gggcgatttt cggtctgact
    49261 cgtggggtga acgctaacca cattatacgc gcgacgctgg agtctattgc ttatcagacg
    49321 cgtgacgtgc tggaagcgat gcaggccgac tctggtatcc gtctgcacgc cctgcgcgtg
    49381 gatggtggcg cagtagcaaa caatttcctg atgcagttcc agtccgatat tctcggcacc
    49441 cgcgttgagc gcccggaagt tcgcgaagtg actgcattgg gtgcggccta tcttgctggt
    49501 ctggcggtcg gtttctggca gaacctcgac gagctgcaag agaaagcggt gattgaacgc
    49561 gagttccgtc caggcatcga aaccactgag cgtaattacc gttacgcagg ctggaaaaaa
    49621 gcggtcaaac gcgcgatggc gtgggaagaa cacgacgagt aatgtaaatg ccggatgaag
    49681 cgtttatgcc gcatccggta gtcctgaaac cgtgcggggg caaccccgca cacatcaata
    49741 atcccaccct tcccctgtgc tacacttcgc gccattcctt actgcttaga gtttgctatg
    49801 agacgagaac ttgccatcga attttcccgc gtcaccgaat ctgcggcgct ggctggctac
    49861 aaatggttag gacgcggcga taaaaacacc gcggacggcg cggcggtaaa cgccatgcgt
    49921 attatgctca accaggtcaa cattgacggc accatcgtca ttggtgaagg tgaaatcgac
    49981 gaagcaccga tgctctacat tggtgaaaaa gtcggtactg gtcgcggcga cgcggtagat
    50041 attgctgttg atccgattga aggcacgcgc atgacggcga tgggccaggc taacgcgctg
    50101 gcggtgctgg cagtaggcga taaaggctgc ttcctcaatg cgccggatat gtatatggag
    50161 aagctgattg tcgggccggg agccaaaggc accattgatc tgaacctgcc gctggcggat
    50221 aacctgcgca atgtagcggc ggcgctcggc aaaccgttga gcgaactgac ggtaacgatt
    50281 ctagctaaac cacgccacga tgccgttatc gctgaaatgc agcaactcgg cgtacgcgta
    50341 tttgctattc cggatggcga cgttgcggcc tcaattctca cctgtatgcc agacagcgaa
    50401 gttgacgtgc tgtacggtat tggtggcgcg ccggaaggcg tagtttctgc ggcggtgatc
    50461 cgcgcattag atggcgacat gaacggtcgt ctgctggcgc gtcatgacgt caaaggcgac
    50521 aacgaagaga atcgtcgcat tggcgagcag gagctggcac gctgcaaagc gatgggcatc
    50581 gaagccggta aagtattgcg cctgggcgat atggcgcgca gcgataacgt catcttctct
    50641 gccaccggta ttaccaaagg cgatctgctg gaaggcatta gccgcaaagg caatatcgcg
    50701 actaccgaaa cgctgctgat ccgcggcaag tcacgcacca ttcgccgcat tcagtccatc
    50761 cactatctgg atcgcaaaga cccggaaatg caggtgcaca tcctctgatt gatttgatcg
    50821 attgagcctt ccagtccttc gggactggaa tttttttgtt cggagaacga agataaggca
    50881 agttaatcaa aacaggagaa aaacatggct gattgggtaa caggcaaagt cactaaagtg
    50941 cagaactgga ccgacgccct gtttagtctc accgttcacg cccccgtgct tccgtttacc
    51001 gccgggcaat tcaccaagct tggccttgaa atcgacggcg aacgcgtcca gcgcgcctac
    51061 tcctatgtta actcgcccga taatcccgat ctggagtttt acctggtcac cgtccccgat
    51121 ggcaaattaa gcccacgatt ggcggcactg aaaccaggcg atgaagtgca ggtggttagc
    51181 gaagcagctg gcttctttgt tctggatgaa gtaccggact gcgaaacgct atggatgctg
    51241 gcaaccggta cagcgattgg cccttattta tcgattctgc aactaggcaa agatttagat
    51301 cgcttcaaaa atctggtcct ggttcacgcc gcacgttatg ccgccgactt aagctatttg
    51361 ccactgatgc aagaactgga aaaacgctac gaagggaaac tgcgcattca gacggtggtc
    51421 agtcgggaaa cggcagcggg gtcgctcacc gggcggatac cggcgttaat tgaaagtggg
    51481 gaactggaaa gcgcgattgg cctgccgatg aataaagaaa ccagccatgt gatgctgtgc
    51541 ggcaatccac agatggtgcg cgatacacaa cagttgctga aagagacccg gcagatgacg
    51601 aaacatttac gtcgccgacc gggccatatg acagcggagc attactggta agcggttact
    51661 tatcgataaa cggcacgatg agcaaatccg cactcatctt attgatcatc ccgcgatatg
    51721 ccggcatcaa acggttgata aatgagtgat gatgaccaca gacaaggagg tcgcactgct
    51781 ctttttgcat aatttccagc agtgtttccg gcatttctcc gcgttcaata cgcagttttg
    51841 tcttcggcca ttgaatattt ttcgtcagtt tatacagctt gttatccgac ttattcttca
    51901 acaattgaag aatatcttct gttgcaggga agtagatacc cggatacaac tcgcttaagc
    51961 catcatcaat atgaattaac gtcaggtgag cgtcattatg tctggcgagc tccagagctt
    52021 tattcaccag taaggcatct tcttcattcc cggaaattgc cacgccaatg tgtttataag
    52081 ccatgtttaa ctccttctaa agcaactcca tcaagctaac gaacgcagtg ataactcaaa
    52141 aataatcatc tcggcctggc atgagaaagt gaaggccgca tcaagctcaa cttgcccctc
    52201 cagatcctta atcttgtagg ccacgctggc cccttcatct gcagaggatg cagcggcggc
    52261 aagctctttc agtttggtca taaaagactc tgcttcttcg cgggtggcaa aaaagcgcga
    52321 aaacttactg gtacagttgt cgttatcaat caccgtaccg atatcaatcg cacatccttt
    52381 agtactgcat ttatctacga catctttcat agggcacctc tgtatattcg cctctctgtc
    52441 gattacgact taagaataat gcgccgttaa gggaagaaca aacaagtaat gatcttatgg
    52501 attaccattt tttttgatat tgcttaaggt gaagagttca gaaggatttt ccgggcattg
    52561 tcatttgcat cgtggaaata acaatcccgg aaaaaaatag cagtaagaga tatattaaag
    52621 gttaattact tgatttattg tcggctttat acttcacatc ctgggtatct ttaccatatt
    52681 tattttcgcc ttgtgtgcca atgaacgctc caagatcgat aagcatcatc accagaatca
    52741 acgtcgggac aaaacgcccc accgcccatt gccagacacc cggtaaaatc gcccagttac
    52801 ccgccagcag catccacgcc acaatcatca gaaatgccca tgcgccggaa cgcccgcgat
    52861 catgcaagcg cttaacggtt actgccgctg ttggccaaag caagcacaca aggcaaaacg
    52921 ccgcggtctg aatatcgagt aaatttttac ccgccagtga gaaaagcacc agcatgcctg
    52981 cgaaccacag gcctatccaa atccagaaat cacggcgtcc aatacgccct ttaaatgaga
    53041 ataaccattg ctgtatggtc atgtaagttc cttgatggtt gtcttttcca ggattctacc
    53101 cttttgacaa gggcgacaga tatcgtttta atcggagcca gtcataacaa aaggtactgt
    53161 caatgaagcc agggtgtacg ctgttttttc tcttatgttc tgcattaacc gttacaacaa
    53221 cggcgcatgc acaaacacca gatacggcaa cgaccgcgcc ttatctgctg gctggagccc
    53281 ctactttcga tctctccatc agccagtttc gagaagactt taacagccag aatcccagcc
    53341 tgccactgaa cgaatttcgt gccatcgaca gcagtcccga caaagccaat ctcactcgtg
    53401 ctgcaagtaa aattaacgag aacttgtatg cttctacagc gctggagcgc ggtaccttaa
    53461 aaatcaaaag cattcaaatg acctggctac ccatccaggg gccagagcaa aaagcagcga
    53521 aagcgaaagc tcaggaatac atggcagcag tgatccgcac actcacccca ttaatgacca
    53581 aaacacaaag ccagaaaaaa ctgcagtcgc tgctaacggc ggggaaaaac aaacgttatt
    53641 acaccgagac agaaggtgca ctgcgttatg ttgtcgcgga caacggcgaa aaggggctga
    53701 ccttcgctgt tgaaccgatt aagctggcgc tatctgaatc gcttgaaggt ttgaataaat
    53761 gacaaaaagc aaagcctttg tgccgatgaa tctctatact gtttcacaga cctgctgccc
    53821 tgcggggcgg ccatcttcct ttattcgctt ataagcgtgg agaattaaaa tgcgacatcc
    53881 tttagtgatg ggtaactgga aactgaacgg cagccgccac atggttcacg agctggtttc
    53941 taacctgcgt aaagagctgg caggtgttgc tggctgtgcg gttgcaatcg caccaccgga
    54001 aatgtacatc gatatggcga agcgcgaagc tgaaggcagc cacatcatgc tgggtgcgca
    54061 aaacgtggac ctgaacctgt ccggcgcatt caccggtgaa acttctgccg ctatgctgaa
    54121 agacatcggc gctcagtaca tcatcatcgg ccactctgaa cgtcgtactt accacaaaga
    54181 gtctgacgaa ctgatcgcga aaaaattcgc ggtgctgaaa gagcagggcc tgactccggt
    54241 tctgtgcatc ggtgaaaccg aagctgaaaa cgaagcgggc aaaactgaag aagtttgcgc
    54301 acgtcagatc gacgcggtac tgaaaactca gggtgctgcg gcattcgaag gtgcggttat
    54361 cgcttacgaa cctgtatggg caatcggtac tggcaaatct gcaactccgg ctcaggcaca
    54421 ggctgttcac aaattcatcc gtgaccacat cgctaaagtt gacgctaaca tcgctgaaca
    54481 agtgatcatt cagtacggcg gctctgtaaa cgcgtctaac gctgcagaac tgtttgctca
    54541 gccagatatc gacggcgcgc tggttggcgg tgcttctctg aaagctgacg ctttcgcagt
    54601 aatcgttaaa gctgcagaag cggctaaaca ggcttaagtc tgacaggtgc cggatttcat
    54661 atccggcact tactttcctt aactcttcgc cttaacgcaa aatctcacac tgatgatcct
    54721 gaatttcctc ggctgaagca cggttaagcg tcagtagatt tcgttgtgtc gccagcaata
    54781 caaatgagtt atcactctgc cgtaccatcg ccagcccgta gcttcccata tgttcccgcg
    54841 cctcaggtac ttcttctgcc agcatcataa atgggctgcg ttgtaccagt tcgctttccg
    54901 ttacccgacg cgccaggtat tcatgcccgc gcaaaccacc tggcagtggc aaccagcggc
    54961 tgctgatgtt cgccagattg ttatccagtt gttcgcgcac atcaggacga atacaagaga
    55021 tatgaatatg aaaatggttt tgcgtacgcc cggtgcggga gttgatcgcc aaagaaaccg
    55081 cgcgatcggg aaccggctgg ccgtattttt tgctcataaa atcacgcgcc tgccaggcca
    55141 accaaaagaa gttcggcgtt gaaggatcgg tcaacaaagg actttcagta ccgttaatac
    55201 gatacgttgg catcaacaga tattgcagtg gaccattaag atcttttaaa accacgtatc
    55261 cggcattggg tttgacttcc gcacatggcg aaggattttg attttgctgc tgattgggca
    55321 aacattcctc aaggacaatc ttacgtaatg tatccgactc ttcaccggtt aatttccagt
    55381 aaccaatacc ggcagccaca acggcgataa ctatcatcac caaaaaaaga agacccgctt
    55441 ttttcatctt tttttccctg tacctcaaag agatgcaagg gtaacgcaaa atcgtgacaa
    55501 ataaaaaacc cggtcacgag aaaccaccgg gtttgataat tatcctgggg aaatcagcgt
    55561 ttgctgatct gatcgaacgt accgccgtta gcaaaatgct ctttttgcgc tttcgtccag
    55621 ccgccgaact cttcatcaat ggtgaataac ttcagctttg gaaacgcatt ttcgtacttt
    55681 ttcgccacct cagcgtcgcg cggacggtag tagtttttcg cggcaatttc ctgaccttct
    55741 ggcgagtaga gatatttcag gtaggcttcc gccacctctt tggtgccttt tttctcgacc
    55801 actttatcga ccaccgacac ggttggctct gcgaggatag actcactcgg cgtgacgatt
    55861 tcgaatttat ctttccccag ttcattcgct gccagcagag cttcgttttc ccaggcgatc
    55921 agtacatcgc caatcccgcg ctcgacaaag gtgttagtgg agccgcgcgc gccagaatcc
    55981 agaacttcga cgtttttata cagtgcccga acaaaatcct gtgcttttgc ctgatcgttg
    56041 ttgttgtgat gcagcgcgta gccccaggca gccaggtagt tccagcgtgc gccaccagag
    56101 cttttcggat taggcgtgat caccgaaaca cccggtttaa tcagatcgtt ccagtcatgg
    56161 atctgcttcg gattgccctt acgcaccagg aaaacaatgg tggaagtgta cggtgcggag
    56221 ttatccggca gacgtttgat ccactcttta tcaatccgcc cgcgttccgc aattgcgtcc
    56281 acgtcatagg ccagagccag cgtgacaaca tcagcttcaa taccgttgat taccgacgtc
    56341 gcttgtttac ctgaaccacc gtgtgactga cgaatcacca cgttatcacc agtttgctgt
    56401 ttccagtggg cgctgaatgc cttgttgtac tgttcgtaca attcgcgcgt tggatcatat
    56461 gaaacgttaa gaagctgaat atcctttgcc ataacgctgg ttgccgccag caaaaatgtt
    56521 aaccctacgc cccacttgtt catcgcccga ctctcttatg ttgtgttgtg atgagcaaag
    56581 cgtgccagaa ggttaaccaa acattaaaga ataaaaaaag attggctata actttcgggt
    56641 atatgttgag ggattaaaaa ggcggaagaa atccgcctca tattgctgac aaagtgcgct
    56701 ttgtccatgc cggatgcggc gcgaacgcct tatccggcct acaaaagttt gcaaattcaa
    56761 taaattgcag aattcatgta ggcctgataa gcgaagcgca tcaggcattt ttgcttctgt
    56821 catcggtttc agggtaaagg aatctgcctt tttccgaaat cattaataca gttttttcgc
    56881 gcagtccagc cagtcgcctt tgaacggacg cttcatgttt tcgatagcgt cgatgatgtc
    56941 gtggtgaacc aactgttcgt tctggatacc tacgcaacga ccgccgtaac ctgccagcag
    57001 cagatcgata gcgtaagcgc ccatacggga agccagaata cggtcgtaag gcaccggaga
    57061 accaccgcgc tggatgtggc ccagcacagt tgcgcgggtt tcacgaccgg tttctttctc
    57121 gatgaaatgc gccagttcgt caacatcaca catatgttcg gtaatcgcca cgatcgcgtg
    57181 ttttttacct ttcgcgatac ccgctttgat ttcgtttacc aggtcttcac ggctgaattc
    57241 aacttccgga accacaacga attcacagcc accggcaatg gccgcagcca gggtcagatc
    57301 gccacaataa cggcccatca cttccaccac ggaaatacgc tggtgagaag aagaggtgtc
    57361 acgcagacgg tcgatcgctt ctacaacggt gctcagcgca gtgaagaaac cgatagtgta
    57421 gtcagtgcct ttgatgtcgt tgtcgatagt gcccggcaga ccgatgcacg ggaagcccat
    57481 ttcggtcaga cgcattgcac ccatgtagga accgtcaccg ccgataacca ccagcgcgtc
    57541 gataccacgt tttttcaggt tttcgatagc cacggcgcgg atgttctcgt cgcggaattc
    57601 cgggaaacgc gcagaaccga ggaacgtacc gccacggttg atcatgtcag acacgctgta
    57661 acggtctagc tgtaccatac ggtcttcata cagacccaga tagccgtcat aaatacccat
    57721 tacttccaga ccttctgtca gcgcagaacg aacaaccccg cgaattgcgg cgttcatgcc
    57781 tggcgcatca ccgccgcttg tcaacacacc gattttctta atcatgacta cctctgaact
    57841 ttggaatgca aaatgaaatc tgttgccgga agtcttcttg cacatcgaag tgatccaacg
    57901 aatgtgcaaa tagtataaca atcacttcct gctgaattga ttcaggtcag gccaaatggc
    57961 ggtattttat acacaaaatg cgggtctggc tctcttttat actgattatg aaagcataga
    58021 ccgtttaccc tccctgggta cgacggaaca ggggtcctga tggataatta catccgatcc
    58081 cggaaaacgc cgtaaaatag cctgctctac ctgatccgcc accatatgtg cctgaaccaa
    58141 aggcagagag tcttccattt ccaaatgaat ctgaataaag cgggtcggcc ctgactgccg
    58201 cgtgcgaaga tcgtgagcgc cgctaacacc cggccaggaa gtcacgatat caataatttc
    58261 ttgccgttcc tcatcaggca atgcgcgatc cagtaatgac tgtaccgcct catatcccat
    58321 gcgtaacgcg ctatataaaa tatagatgcc gattcccaat gcaaacagag catcggcgcg
    58381 atgccagccg taccaggaca accccagcgc cagcagaatt gcgccgttca tcataacatc
    58441 agactggtaa tgtagcatat cagcccgcac cgcctggctt tgcgtccgcc gcaccaccca
    58501 acgctgaaac gagacaagga taatcgtaca aattagcgcc acaattgtca cgataacccc
    58561 gacgcctgga tctgtcatcg gtgttggaga tatcagatgt tgaatacccg tcaaaaacag
    58621 gaatagtgcc gaaccggaga taaacatact ttgcgccagc gccgcgaggg actcagcttt
    58681 accgtgacca aacgagtgat tatcgtcggc aggttgcagg gaatatcgca ccaccaataa
    58741 attcgtcaac gacgcgccga tatccaccag cgaatccacc agcgcggcga gaatactcac
    58801 cgacccggta taccaccatg caaaaatttt aatcagcaat agcagcgaag ccatcgccgt
    58861 cgcagcaatc gccgcccgac tgaccagccg tccataagat tgattcataa atactcccgc
    58921 tatcaactga cgctagtata acggaagcaa atcatctgca atgcattaag cagcaggcaa
    58981 attgaggata aaaaaaaccc ccacatcatg tgggggaaga cagggatggt gtctatggca
    59041 aggaaaacag ggtttactac tgggaacgtg agttgctact actcaatagc ttcaacgatg
    59101 aacttttttg ccattgcgtc acgtcacgca actgctccat tcgttgttga tgtttctcgt
    59161 ttaaaaccgc ttgctgctcc ggcgttaaca ggcgatacat ttggttgcgg acttttgcca
    59221 tctcaacctg acgagcaatt tgctcattcg ccattttttc tgcctgtgcg cgcacagcgt
    59281 tttcatcaaa attttctgcg gtgacaaggc gatgcattgt ctccagttcg ctaacattaa
    59341 caggaggctg ttcgtgccgg gcctgttgca taagatctcg catctgctga cgctgatgtt
    59401 cggttaaact tatgccgtcg aacatatggc tctgcgtact gcgctgcgta agttcttcac
    59461 ccggatgcca gttatcgcct gaaccgactt cagcagcgtg gcttaatgaa ctgactgcca
    59521 gcgttgaggc catgacggca gcggtaacta tgcgcatcat ttgctcccaa aatctttctg
    59581 tcgcgattca acgatagaga gtttacgatt caggctgcaa acatgcgtca gggggtgtaa
    59641 aacaacgtaa agtcatggat tagcgacgtc tgatgacgta atttctgcct cggaggtatt
    59701 taaacaatga ataaaatcct gttagttgat gatgaccgag agctgacttc cctattaaag
    59761 gagctgctcg agatggaagg cttcaacgtg attgttgccc acgatgggga acaggcgctt
    59821 gatcttctgg acgacagcat tgatttactt ttgcttgacg taatgatgcc gaagaaaaat
    59881 ggtatcgaca cattaaaagc acttcgccag acacaccaga cgcctgtcat tatgttgacg
    59941 gcgcgcggca gtgaacttga tcgcgttctc ggccttgagc tgggcgcaga tgactatctc
    60001 ccgaaaccgt ttaatgatcg tgagctggtg gcacgtattc gcgcgatcct gcgccgttcg
    60061 cactggagcg agcaacagca aaacaacgac aacggttcac cgacactgga agttgatgcc
    60121 ttagtgctga atccaggccg tcaggaagcc agcttcgacg ggcaaacgct ggagttaacc
    60181 ggtactgagt ttaccctgct ctatttgctg gcacagcatc tgggtcaggt ggtttcccgt
    60241 gaacatttaa gccaggaagt gttgggcaaa cgcctgacgc ctttcgaccg cgctattgat
    60301 atgcacattt ccaacctgcg tcgtaaactg ccggatcgta aagatggtca cccgtggttt
    60361 aaaaccttgc gtggtcgcgg ctatctgatg gtttctgctt catgataggc agcttaaccg
    60421 cgcgcatctt cgccatcttc tggctgacgc tggcgctggt gttgatgttg gttttgatgt
    60481 tacccaagct cgattcacgc cagatgaccg agcttctgga tagcgaacag cgtcaggggc
    60541 tgatgattga gcagcatgtc gaagcggagc tggcgaacga tccgcccaac gatttaatgt
    60601 ggtggcggcg tctgttccgg gcgattgata agtgggcacc gccaggacag cgtttgttat
    60661 tggtgaccac cgaaggccgc gtgatcggcg ctgaacgcag cgaaatgcag atcattcgta
    60721 actttattgg tcaggccgat aacgccgatc atccgcagaa gaaaaagtat ggccgcgtgg
    60781 aactggtcgg tccgttctcc gtgcgtgatg gcgaagataa ttaccaactt tatctgattc
    60841 gtccggccag cagttctcaa tccgatttca ttaacttact gtttgaccgc ccgctattac
    60901 tgctgattgt caccatgttg gtcagtacgc cgctgctgtt gtggttggcc tggagtctgg
    60961 caaaaccggc gcgtaagctg aaaaacgctg ccgatgaagt tgcccaggga aacttacgcc
    61021 agcacccgga actggaagcg gggccacagg aattccttgc cgcaggtgcc agttttaacc
    61081 agatggtcac cgcgctggag cgcatgatga cctctcagca gcgtctgctt tctgatatct
    61141 ctcacgagct gcgcaccccg ctgacgcgtc tgcaactggg tacggcgtta ctgcgccgtc
    61201 gtagcggtga aagcaaggaa ctggagcgta ttgaaaccga agcgcaacgt ctggacagca
    61261 tgatcaacga tctgttggtg atgtcacgta atcagcaaaa aaacgcgctg gttagcgaaa
    61321 ccatcaaagc caaccagttg tggagtgaag tgctggataa cgcggcgttc gaagccgagc
    61381 aaatgggcaa gtcgttgaca gttaacttcc cgcctgggcc gtggccgctg tacggcaatc
    61441 cgaacgccct ggaaagtgcg ctggaaaaca ttgttcgtaa tgctctgcgt tattcccata
    61501 cgaagattga agtgggcttt gcggtagata aagacggtat caccattacg gtggacgacg
    61561 atggtcctgg cgttagcccg gaagatcgcg aacagatttt ccgtccgttc tatcggaccg
    61621 atgaagcacg cgatcgtgaa tctggcggta caggattggg gctggcgatt gttgaaaccg
    61681 ccattcagca gcatcgtggc tgggtgaagg cagaagacag cccgctgggc ggtttacggc
    61741 tggtgatttg gttgccgctg tataagcgga gttaaactcc gcatttgtag gcaggataag
    61801 gcgtttacgc cgcatccggc atttgagcag gatgcctgat gcgacgctga tagcgtctta
    61861 tcaggcctac actccggggg ttattttccc cacaaccggc gggaaaaatc ttcgatcttg
    61921 ccgctcagtc ggcgcttttg catcgtccgc gtccagcttt tcgataaccc ggcggcggag
    61981 agtaaacggt gatattgatc gtcatcaaac ggcatatgcc atgcgatggc ggcagcctcc
    62041 tgcacggtca catcgctgac acgggaaacc aactccagcg gcgcgtccgc agacactttc
    62101 cctggcgcta tcacgctata caaccacccc accttacctg tgttttgcat caactgcgct
    62161 atatcgctga tatcaaaatg gtaattgagt ttatagcaag gcgagcgcgg ctggctgacc
    62221 tgaattaatg cctctcccca gcggaaaata tcgcccatat aaacattact ttccgtcagg
    62281 ccgtcggttg agaggttttc accaaacgca ggcgcaacaa acaactccgc ctgttccgga
    62341 aattcccgcg cccagtagag ataatgctca cgaggataat gacacagcgc tctgtctggc
    62401 ccgccgtgaa cctttttctc cgcctgctcg tcaccttcca gccccagctc cgtcagcatc
    62461 aactcaccat caacctggat tttggcaatt gcgctgggtt tgccttcggg ataagcctga
    62521 atcttgcctg tgtatacatc aaccggatat cgcatctgtt ccctctccac gagaaatttc
    62581 cccattagac catatttatt ttatgtcagg cgtgctgttt tcgcggtaag tatccaggca
    62641 ggaactcaat agcaaaccgg acgaacgtta gttcatccgg ctgtggggat taaggaagca
    62701 ggtttatttc accgtaccta aaatttcgat tttcgctgct ctggctttga ctttacgtga
    62761 ccagatagag gtaagaattg gcaccagaat cgaagtcaca atgaccgccg tcgctaccag
    62821 cgaagttgct gccggagcca tcggtttaaa cgcaggcacc atttctgcaa tcagcacagg
    62881 tgtcgctacc gcggcccctg cggaactgga agcggcaatt ccggcagtgc catcgccacc
    62941 gccaatcaat ttatcggcga taatcaacgg aataccggtc acgataatta ctgccacacc
    63001 caacaggatc cccagcaaac cagtctgggc aatcacagtc aaatcaatgg tattgcccag
    63061 cgcgaaggca aagaatggaa tcagcgtttg caccgctttg ctgaaaaatt ctcgtaattc
    63121 agggtcaagg ttccccaggg caaagcccac caggaacggt aatactgcgc cgacgaaaac
    63181 atgcggttca aacgaggcaa tccctgcagt gcccagaata atcatcgtca tgagcggccc
    63241 ggactccaac gacatcaaca caaatgcccc agcttcttct tttgtgccgt actgctgcat
    63301 gatggaagcg taaagtccgc cattggtcat atccatcgcc gccaccagcg ccagcgttga
    63361 aagtccggca aagaatccaa cttcaacacc atgttccgga ataatgcgcg aggcaattgc
    63421 cgcaaccacc cacgcgacgg caatttttgt taccaccagc gtaccggatt tacgcaatac
    63481 cgttcccgtc gcgcttaatt ttattgacgc ccccatgcaa aaaaaccaca ccgccagaat
    63541 gggcaccgta ccggtaatca tcccgttggt gaatgatcca aaatatttcc ccgcccccgg
    63601 cgagaaggtg tggcacagtg cgccaaggaa tagcgggacg agcatcatcc cccccgggat
    63661 tttctcaatc gagcgtttta tctgcatttc catcacctgt cggcatattt ttaagggctg
    63721 atgcgtaatt cctcaatgga gcatcagtgt tgatggaaac gaagatagac attcatttaa
    63781 tcaataaaag tgatcaaaac cacatcaatt gaaacactgt tttattttta tcggatcatt
    63841 gtttttattt tgaaactgat cacaaaaaaa ccaccgccgt tggcgatggt tcatttttta
    63901 agctgatatg cggcgtatac gcctcattgc agcaggcggc aaataattat tttttcgccg
    63961 cgaaacgtgc cgatgcttcg tcccagttca ccacgttcca gaactctttg atgtagtccg
    64021 gacggcggtt ctggaatttc aggtagtaag catgttccca cacatccagg cccagaatcg
    64081 ggaagccgga agcgccagaa atagcttcac ccatcagcgg agaatcctgg ttagcagtag
    64141 aaaccaccgc cagtttatcg cctttcagca ccagccatgc ccagccggaa ccaaagcggg
    64201 aagctgccgc tttctcaaat tctgctttga agttatcaac ggagccgaag tcacgttcga
    64261 tagccgcttt caggtcaccc tgcagggtgg tgcctttttt cagacctttc cagaacaggc
    64321 tgtggttagc gtgaccgcca gcgttgttgc gcagtacggt tttcttgtct gctggcagct
    64381 ggtccagttt ggtgatcagc tcttcaaccg gcaggttggc aaattctggc aggctttcca
    64441 gcgccgcgtt ggcgttgttg acgtaggtct gatggtgttt ggtgtggtgg atttccatgg
    64501 tctgcttatc gaagtgcggt tccagggcat cgtaagcata cggcagggat ggcagggtat
    64561 agctcatatt catctccagt attgtcgggc ggccgattgt taatgccgcg taagcagttg
    64621 gttcattata gttaattaaa tgatattgaa aatgattatc aatgccgtac ttttcgtaag
    64681 ggtatggttt tgcaggaaaa tgcccgagat gtgaagcaaa tcacccactt aatgccgtga
    64741 ttgccagtaa atcgacaacg gcggcaacag gcgaaaggtt aatcgacagc acgattttta
    64801 cactcatctc gtcggagatg tgacgcgacg aaaaatgatg aggataagaa gatgagtaac
    64861 gcgattacga tggggatatt ttggcatttg atcggcgcgg ccagtgcagc ctgtttttac
    64921 gctccgttca aaaaagtaaa aaaatggtca tgggaaacca tgtggtcagt cggtgggatt
    64981 gtttcgtgga ttattctgcc gtgggccatc agcgccctgt tactaccgaa tttctgggcg
    65041 tattacagct cgtttagtct ctctacgctg ctgcctgttt ttctgttcgg cgctatgtgg
    65101 gggatcggta atatcaacta cggcctgacc atgcgttatc tcggcatgtc gatgggaatt
    65161 ggcatcgcca ttggcattac gttgattgtc ggtacgctga tgacgccaat tatcaatggc
    65221 aatttcgatg tgctgattaa caccgaaggc ggacgcatga cgttgctcgg cgttctggtg
    65281 gcgctgattg gcgtagggat tgtaactcga gccgggcagt tgaaagagcg caagatgggc
    65341 attaaagccg aagagttcaa tctgaaaaaa gggctggtgc tggcggtgat gtgcggcatt
    65401 ttctctgccg ggatgtcctt tgcgatgaac gccgcaaaac cgatgcatga agccgctgcc
    65461 gcactaggcg tcgatccact gtatgtcgct ctgccaagct atgtcatcat catgggcggc
    65521 ggcgcgatca tcaacctcgg tttctgtttt attcgtctgg caaaagtgaa ggatttgtcg
    65581 ctaaaagccg acttctcgct ggcaaaaccg ctgatcattc acaatgtgtt actctcagca
    65641 ctgggcgggt tgatgtggta tctgcaattc tttttctatg cctggggcca cgcccgcatt
    65701 ccggcgcagt atgactacat cagttggatg ctgcatatga gtttctatgt attgtgcggc
    65761 ggtatcgtcg ggctggtgct gaaagagtgg aacaatgcag gtcgccatcc ggtaacggtg
    65821 ttgagcctcg gttgtgtggt gattattgtc gccgccaaca tcgtcggcat cggcatggcg
    65881 aattaatctt tctgcgaatt gagatgacgc cactggctgg gcgtcatccc ggtttcccgg
    65941 gtaaacacca ccgaaaaata gttactatct tcaaagccac attcggtcga aatatcactg
    66001 attaacaggc ggctatgctg gagaagatat tgcgcatgac acactctgac ctgccgcaga
    66061 tattgattga tggtcattcc agtctgctgg cgaaattgct gacgcaaaac gcgctcactg
    66121 cacgatgcct catcacaaaa tttatccagc gcaaagggac ttttcaggct agccgccagc
    66181 cgggtaatca gcttatccag caacgtttcg ctggatgttg gcggcaacga atcactggtg
    66241 taacgatggc gattcagcaa catcaccaac tgcccgaaca gcaactcagc catttcgtta
    66301 gcaaacgaca catgctgact actttcatgc tcaagctgac cgataacctg ccgcgcctgc
    66361 gccatcccca cgctacctaa gcgccagtgt ggttgccctg cgctggcgct aaatcccgga
    66421 atcgctccct gccagtcaag attcagcttc agccgctccg ggcaataaat aatattctgc
    66481 aaaaccagat cgttaacgga agcgtaggag tgtttatcgt cagcatgaat gtaaaagaga
    66541 tcgccacggg taatgcgata agggcgatcg ttgagtacat gcagaccatt accgcgccag
    66601 acaatcacca gctcacaaaa atcatgtgta tgttcggcaa aaacatcttg cggataacgg
    66661 tcagccacag cgactgcctg ctggtcgctg gcaaaaaaat catctttgag aagttttaac
    66721 tgatgcgcca ccgtggctac ctcggccaga gaacgaagtt gattattcgc aatatggcgt
    66781 acaaatacgt tgagaagatt cgcgttattg cagaaaacca tcccgtccct gacgaatatc
    66841 acgcggtgac cagttaaact ctcggcgaaa aagcgtcgaa aagtggttac tgtcgctgaa
    66901 tccacagcga taggcgatat cagtaacgct ggcctcgctg tggcgtagta gatgtcgggc
    66961 tttcatcagt cgcaggcggt tcaggtaacg ctgaggcgtc agtcccgttt gctgcttaag
    67021 ctgccgatgt agcgtacgca gcgaaagaga aaattgctcc gccacggcat cccaattcac
    67081 ctcatcggca aaatggtcct ccagccaggc cagaagcaag ttgagacgtg atgcgctgtt
    67141 ttccaggttc tcctgcaaac tgcttttacg cagcaagagc agtaattgca taaacaagat
    67201 ctcgcgactg gcggtcgagg gtaaatcatt ttccccttcc tgctgttcca tctgtgcaac
    67261 cagctgtcgc acctgctgca atacgctgtg gttaacgcgc cagtgggaag gatactgccc
    67321 atccagctct tgtggcagca actgattcag cccggcgaga aactgaaatc gatccggcga
    67381 gcgatacagc acattagtca gacacagatt atcggtatgt tcatacagat gccgatcatg
    67441 gtcgcgcaca aaacagaccg tgccaccggt gatggtatag ggctgcccat taaacacatg
    67501 aatacccgtg ccatgttcga caatcacaat ttcatgaaaa tcatgatgat gttcaggaaa
    67561 atccgcctgc gggagccggg gttctatcgc cacggacgcg ttaccagacg gaaaaaaatc
    67621 cacactatgt aatacggtca tactggcctc ctgatgtcgt caacacggcg aaatagtaat
    67681 cacgaggtca ggttcttacc ttaaattttc gacggaaaac cacgtaaaaa acgtcgattt
    67741 ttcaagatac agcgtgaatt ttcaggaaat gcggtgagca tcacatcacc acaattcagc
    67801 aaattgtgaa catcatcacg ttcatctttc cctggttgcc aatggcccat tttcctgtca
    67861 gtaacgagaa ggtcgcgaat tcaggcgctt tttagactgg tcgtaatgaa attcagcagg
    67921 atcacattat gacctttcgc aattgtgtcg ccgtcgatct cggcgcatcc agtgggcgcg
    67981 tgatgctggc gcgttacgag cgtgaatgcc gcagcctgac gctgcgcgaa atccatcgtt
    68041 ttaacaatgg gctgcatagt cagaacggct atgtcacctg ggatgtggat agcctggaaa
    68101 gtgccattcg ccttggatta aacaaggtgt gcgaggaagg gattcgtatc gatagcattg
    68161 ggattgatac ctggggcgtg gactttgtgc tgctcgacca acagggtcag cgtgtgggcc
    68221 tgcccgttgc ttatcgcgat agccgcacca atggcctgat ggcgcaggca caacaacaac
    68281 tcggcaaacg cgatatttat caacgtagcg gcatccagtt tctgcccttc aatacgcttt
    68341 atcagttacg ggcgctgacg gagcaacaac ctgaacttat tccacacatt gctcacgctc
    68401 tgctaatgcc ggattatttt agttatcgcc ttaccggcaa gatgaactgg gagtacacca
    68461 acgccacaac cacgcaactg gtcaatatca atagcgacga ctgggacgag tcgctactgg
    68521 cgtggagcgg tgccaacaaa gcctggtttg gtcgcccgac gcatccgggt aatgtcatag
    68581 gtcactggat ttgcccgcag ggtaatgaga ttccggtggt cgccgttgcc agccatgata
    68641 ccgccagcgc ggttatcgcc tcgccgttaa acggttcacg cgccgcttat ctctcttctg
    68701 gtacctggtc attgatgggc ttcgaaagcc agacgccatt taccaatgac acggcgctgg
    68761 cagccaacat caccaatgaa ggcggggcgg aaggtcgcta tcgggtgctg aaaaatatta
    68821 tgggcttatg gctgcttcag cgggtgctta aggagcggaa aatcaacgat ctcccggcgc
    68881 ttatcgtcgc gacacagtca cttccggcct gccgcttcat catcaatccc aatgacgatc
    68941 gctttattaa tcctgaggcg atgtgcagcg aaattcaggc tgcgtgtcgg gaaacggcgc
    69001 aaccgatccc ggaaagtgat gctgaactgg cgcgctgcat tttcgacagt ctggcgctgc
    69061 tgtatgccga tgtgttgcat gagctggcgc agctacgcgg tgaagatttc tcgcaactgc
    69121 atattgtcgg cggaggctgt cagaacacgc tgctcaacca gctatgtgcc gatgcctgcg
    69181 gtattcgggt gatcgccggg cctgttgaag cctcgacgct cggcaatatc ggcatccagt
    69241 taatgacgct ggatgaactc aacaatgtgg atgatttccg tcaggtcgtc agcaccaccg
    69301 cgaatctgac cacctttacc cctaatcctg acagtgaaat tgcgcactac gtggcgcgga
    69361 ttcactctac acgaaagaca aaggagcttt gcgcatgacc actcaactgg aacaggcctg
    69421 ggagctggcg aaacagcgtt tcgcggcggt ggggattgat gtcgaggagg cgctgcgcca
    69481 acttgatcgt ttacccgttt caatgcactg ctggcagggc gatgatgttt ccggttttga
    69541 aaacccggaa ggttcattga ccggtgggat tcaggcaact ggtaattatc cgggcaaagc
    69601 gcgtaatgcc agtgagctac gtgccgatct ggaacaggct atgcggctga ttccggggcc
    69661 gaaacggctt aatttacatg ccatctatct ggaattagac acgccagtct cgcgcgatca
    69721 gatcaaacca gagcacttca aaaactgggt tgaatgggcg aaagccaatc agctcggtct
    69781 ggattttaac ccctcctgtt tctcgcatcc gctaagcgcc gatggcttta cgctttccca
    69841 tgccgacgac agcattcgcc agttctggat tgatcactgc aaagccagcc gtcgcgtttc
    69901 ggcctatttt ggcgagcaac tcggcacacc atcggtgatg aacatctgga tcccggatgg
    69961 tatgaaagat atcaccgttg accgtctcgc cccgcgtcag cgtctgctgg cagcactgga
    70021 tgaggtgatc agcgagaagc taaaccctgc gcaccatatc gacgccgttg agagcaaatt
    70081 gtttggcatt ggcgcagaga gctacacggt tggctccaat gagttttaca tggggtatgc
    70141 caccagccgc cagactgcgc tgtgcctgga cgccgggcac ttccacccga ctgaagtgat
    70201 ttccgacaag atttccgccg ccatgctgta tgtgccgcag ttgctgctgc acgtcagccg
    70261 tccggttcgc tgggacagcg atcacgtagt gctgctggat gatgaaaccc aggcaattgc
    70321 cagtgagatt gtgcgtcacg atctgtttaa ccgggtgcat atcggccttg acttcttcga
    70381 tgcctctatc aaccgcattg ccgcgtgggt cattggtaca cgcaatatga aaaaagccct
    70441 gctgcgtgcg ttgctggaac ctaccgctga gctgcgcaag ctggaagcgg cgggcgatta
    70501 cactgcgcgt ctggcactgc tggaagagca gaaatcgttg ccgtggcagg cggtctggga
    70561 aatgtattgt cagcgtcacg ataccccagc aggtagcgaa tggctggaga gcgtgcgggc
    70621 ttatgaaaaa gaaattttga gccagcgtgg gtaaacactg ccggatgcgg cgcgagcgcc
    70681 ttatccggcc tacgggtcga atgtccatgc gggctgtcac cctcaccccg gccctctccc
    70741 tgaaagggcg agggagaaaa acatagcctc gtgcggcaac ggagctgaac accgctccgg
    70801 acagtcccct cgcccctacg gggagagggt tagggtgagg ggaacaagcc gcaccatgtc
    70861 taaacaaggc aacctaattt acaggaacac agaacatgca aaacattacc cagtcctggt
    70921 ttgtccaggg aatgatcaaa gccaccaccg atgcctggct gaaaggctgg gacgagcgca
    70981 acggcggcaa cctgacgctg cgcctggatg acgccgatat cgcgccttat aaagacaact
    71041 tccatgcaca accgcgctat atcccgctca gccagcccat gcctttactg gcaaatacac
    71101 cgtttattgt caccggctcc ggcaaattct tccgtaacgt ccagcttgat cctgcagcta
    71161 acttaggcgt cgtaaaagtc gacagcgacg gcgcgggcta ccatattctc tgggggttaa
    71221 ccaacgaagc cgttccaact tctgaacttc cggctcactt tctctcccac tgcgagcgca
    71281 ttaaagccac caacggtaaa gatcgggtga tcatgcactg ccacgccacc aacctgatcg
    71341 ccctcaccta tgtactggaa aacgacaccg cggtctttac tcgccaactg tgggaaggca
    71401 gcaccgagtg tctggtagtg ttcccggatg gcgttggcat tttgccgtgg atggtgcccg
    71461 gcacggacga aatcggccag gcgaccgcac aagagatgca aaaacattcg ctggtgttgt
    71521 ggcccttcca cggcgtcttc ggcagcgggt cgacgttgga tgaaacattc ggtttaatcg
    71581 acaccgcaga aaaatcagca caagtattag tgaaggttta ttcgatgggc ggcatgaaac
    71641 agaccatcag ccgtgaagag ttgatagcgc tcggcaagcg cttcggcgtt acaccactag
    71701 ctagtgcgct ggcgctgtaa ggagcaaaca tgatccgcaa agcctttgtc atgcaggtta
    71761 accccgacgc ccacgaagag tatcagcgtc ggcataaccc catctggccg gaactggaag
    71821 cagtgttgaa atctcacggt gcgcataact acgccattta cctcgacaaa gcgcgtaatc
    71881 tgctgtttgc cacggtagag attgaatctg aagaacggtg gaatgcggta gccagcactg
    71941 atgtttgcca acgttggtgg aaatatatga ccgatgttat gcccgctaac ccggataaca
    72001 gcccggtgag tagcgagctg caagaagtgt tttacctgcc gtaattgccc aatgtgccag
    72061 agatgcctcc ggcacaatat tttaaatttc gatttgaaaa attaaacata ttacatctcc
    72121 cggtaagtta tatttccctg atacattgtg agtaaatcac aaaaataatg aataacccat
    72181 taatgattca tgtggtttat ttaaataacc attatgtgca ttactccgca aatctgacct
    72241 ttcactctgt ctcaaattgt cattgtttac cgacatatcg cctctcatca cttaatcgct
    72301 ccaccattac aattgattaa aatatatttt atggagtccg attaatggca gctcttactg
    72361 caagctgtat tgacctgaat attcagggca atggcgctta ttccgttctg aagcagttgg
    72421 cgacaatagc gttacaaaac ggttttatca ccgactcaca ccagttcctg caaaccctgc
    72481 tgctgcgcga aaaaatgcac tctactggat ttggttccgg tgtcgccgtg ccgcacggta
    72541 aaagcgcctg cgttaaacaa ccgttcgtat tattcgcccg caaagcgcag gctattgact
    72601 ggaaagccag cgatggcgaa gacgtcaatt gctggatctg cctcggcgtg ccgcaaagcg
    72661 gcgaagagga tcaggtcaaa atcatcggca cactgtgtcg caaaattatt cacaaggaat
    72721 ttattcatca actgcaacag ggcgataccg accaggtgct tgccttgtta aatcaaaccc
    72781 tcagctcata aggaagtggc gatggagtca tccttacgta ttgtcgcgat caccaactgc
    72841 cccgccggga tcgctcacac ctacatggtg gcggaagccc tggaacagaa agcccgttct
    72901 ctcggtcata ccattaaagt tgaaactcaa ggatcaagcg gcgtcgaaaa ccgcttatcc
    72961 agcgaagaga ttgccgctgc cgattacgtc attctcgcta ccgggcgtgg cctgagcggt
    73021 gacgatcgcg cgcggtttgc cgggaagaag gtttatgaga ttgccatctc ccaggcgctg
    73081 aaaaatatcg atcagatttt cagtgaatta ccaacaaact cgcatctttt tgctgcagat
    73141 agcggcgtga agcttggcaa acaggaagtg caaagcggca gcgtgatgag ccacctgatg
    73201 gcgggcgtct ctgcggcgct gccgtttgtc atcggcggcg gtattctggt ggcgcttgcc
    73261 aacatgctgg tgcagttcgg tttgccatat accgatatgt cgaaaggtgc gccgtcgttt
    73321 acctgggtag ttgaatccat cggttacctc ggcttcacct ttatgatccc catcatgggc
    73381 gcttatattg cctcgtcgat tgccgataaa ccggctttcg ctccggcgtt tctggtttgc
    73441 tatctggcga acgacaaagc gctgctcggc acccagtcgg gtgcgggatt cctcggcgcg
    73501 gtggtgctgg ggctggcgat tggctatttc gtcttctggt tccgcaaagt gcgccttggc
    73561 aaagcattac aaccgctgct cggttcaatg ctgatcccgt tcgttactct gctggttttc
    73621 ggcgtgctca cttactacgt tatcgggccg gtgatgtccg acctcatggg cgggctgctc
    73681 cacttcctga acaccattcc tccatcaatg aagtttgcgg cagcgtttct ggtcggtgcg
    73741 atgctggcgt tcgatatggg cggccccatc aataaaaccg cctggttctt ctgcttctca
    73801 ctgctggaaa aacacattta tgactggtac gccatcgtcg gcgttgtcgc gttgatgccg
    73861 cccgtcgcgg cgggtcttgc gacgttcatt gcgcccaaac tgtttacccg acaggaaaaa
    73921 gaggctgcca gtagtgccat tgtggtcggt gccacagtcg caaccgaacc ggctattcct
    73981 tacgctctgg cagctccgtt gccgatgatt accgccaata ccctggcggg cggcattacc
    74041 ggcgtgctgg tgatcgcttt cggaatcaaa cgtctggcgc ctggtttggg tatctttgac
    74101 ccgctgatcg gcctgatgtc gccggtaggt tcgttttatc tggtgctggc gattggcctc
    74161 gcgctgaaca tttcattcat tatcgtcctg aaaggattgt ggttacgccg caaagcgaaa
    74221 gccgcgcaac aggagcttgt ccatgaacat tgagttactg caacagttgt gcgaagccag
    74281 cgccgttagc ggcgatgaac aggaagttcg caacatcctg ataaacacgc tggaaccttg
    74341 cgttaatgag atcacctttg atggtctggg cagctttgtt gcccgtaagg gaaataaagg
    74401 tccaaaagtt gccgttgttg ggcatatgga tgaagtcggc tttatggtca cccacatcga
    74461 cgagagcggt tttctgcgct tcaccaccat tggtggctgg tggaatcagt cgatgctcaa
    74521 ccaccgggta accatacgca cacacaaggg agtgaaaatc cctggtgtga ttggttccgt
    74581 cgcgcctcat gcgttaacgg aaaagcaaaa gcaacaaccg ctgtcatttg atgagatgtt
    74641 cattgatatt ggcgcgaaca gtcgcgaaga ggtggaaaaa cgcggcgtgg aaattggcga
    74701 ttttattagc ccggaagcca attttgcctg ctggggcgaa gataaagtag tcggcaaggc
    74761 gctggataac cgcatcggct gcgcgatgat ggcggagcta ctacagacag taaataaccc
    74821 agaaattacg ctgtatggcg ttggcagtgt ggaagaagaa gttgggctac gcggggcaca
    74881 aacctcggcg gaacacatta aaccggatgt ggtgatcgtg ctggataccg ctgtcgcagg
    74941 tgatgttccg ggcattgata acattaaata cccgctgaaa ctgggccagg ggccggggct
    75001 gatgctgttt gacaagcgct acttccccaa ccagaaactg gtagcagcgt ttaaaaactg
    75061 tgccacgcag aatgatttac cgctgcaatt ttccaccatg aaaaccggag cgacggatgg
    75121 cgggcgctac aacgtgatgg gcggcgggcg tccggttgtc gcgctgtgtc tgccaactcg
    75181 ttatctgcac gccaacagcg gtatgatttc aaaagccgat tacgatgctc tgctcacgct
    75241 gatacggggt tttctgacca ccttaactgc ggagaaagtc aacgcgttta gccagttccg
    75301 tcaggtggat taatgctaaa cgaacgccag ttaaagattg tcgatctgct ggagcaacag
    75361 ccgcgcacgc ctggtgagct ggcgcaacag actggcgttt caggcaggac catcctgcgt
    75421 gatattgact atctcaactt tacccttaac ggcaaagccc gcatttccgc cagtggtagt
    75481 gcgggctatc agctggaaat cttcgagcgc cgcagctttt ttcagttgct gcaaaagcac
    75541 gataacgacg atcggctgct ggcactgtta ttactgaata cctttactcc ccgtgcgcaa
    75601 ctcgcctcgg cgcttaattt gccagaaacg tgggtcgcag agcgtctgcc ccggttaaaa
    75661 cagcgttatg aacgcgcttg ttgcctggcc agccgccccg gtttgggcca tttcattgat
    75721 gagacagaag agaagcgcgt tatcttgctg gcgaacttgc tgcgcaaaga tccgttttta
    75781 attccgctgg cgggcgtaac acgagacaac cttcagcatt tatccacggc ctgcgacaac
    75841 caacaccgct ggccgctcat gcagggtgat tatctctcca gcctgattct ggcgatttac
    75901 gccctgcgta atcaactgac cgatgagtgg ccgcaatatc ccggtgacga gataaaacaa
    75961 atcgttgaac atagcggtct gtttcttggt gataacgctg taagaaccct gacaggtttg
    76021 atagagaaac agcatcagca agcgcagata atttctgccg ataatgtgca gcggttgctg
    76081 caaagggtgc cgggcatcgc gtcattgaat attattgata cgcagctggt tgagaacatt
    76141 accgggcatt tattacgttg ccttgccgca ccggtgtgga ttgctgagca ccgccaaagc
    76201 agcatgaata acctgaaagc cgcctggcct gcggcctttg atatgagtct gcactttatt
    76261 acgctactgc gtgaacagct cgatattccc cttttcgaca gcgatctgat cggtttgtat
    76321 tttgcctgtg cgctggaacg gcatcaaaac gaacgccagc cgatcatttt gctctcggac
    76381 cagaacgcga ttgccactat taatcagctc gccattgagc gcgatgtttt acattgtcgg
    76441 gtgattattg cccgtagctt aagcgaactt gttgccattc gcgaagagat tgagccgtta
    76501 ttgatcatta acaacagcca ttatttactg gatgacgcgg taaataatta catcaccgta
    76561 aaaaatatca ttacggctgc cggtatcgaa caaataaaac attttctggc gacggcattt
    76621 attcgccaac agccggagcg ttttttctct gcccccggaa gttttcatta ttcgaatgta
    76681 cgcggtgaaa gctggcaaca tattacccgg caaatttgtg cgcaattagt cgcacaacac
    76741 catattaccg ccgatgaagc acaacgcatc atcgcccgcg aaggcgaagg tgaaaacctg
    76801 attgttaatc gcctcgccat cccacattgc tggagcgaac aggagcgacg ttttcgtgga
    76861 ttttttatta ccctcgccca accagttgag gtgaataacg aagtcattaa ccatgtcttg
    76921 atcgcctgcg ccgccgccga tgcgcgtcat gagctaaaaa tatttagcta tctggcaagc
    76981 atattgtgtc agcatccggc ggagattatt gccgggttaa caggatatga ggcatttatg
    77041 gagttacttc acaaggggtg aactgtgtcg gatgcgccgc atccgacact gcatttatca
    77101 tcaaaaacgt ttaacgcagg ctattatagt catcaaccat ttcgttgtac tcgcgtaatg
    77161 ctctcgggaa agagtcttcg accatccagc ttgagtttgc gtcctgaagt tgctctttat
    77221 cccagtcaga gtacgggact ttatctctga tgcggcgaac gtatttttta gcctcgagtt
    77281 gatactggcc tgccgaatta ataaacgaga aattcatgcc gcctttatcc gcttctttgg
    77341 cctgcgccac tagcgtttcc agttcagaca ctttcttcat tgccgcatca acatcaaact
    77401 tctcttgcga tatcagatta ttaatttgtt tcgctgaaat catgactgcc agagagtagt
    77461 agtggaatgt tttcccttct ctttcttcaa tatttttcag ttccgcaagc tggcgcttat
    77521 cgttaatttc ctgaatcgcc gcatgataag attctgctac agcttcaaag gcttccagat
    77581 tttttaagaa tgtctggtgc agcgttttcc ctttcgcaaa cgcatcgtct ttataatttt
    77641 cctgggtgta atatttatcc atttcgttaa tggtattacc caacgccacg gcagcatcga
    77701 tataactcac cgccacgcca tcaataggtt gcagcgcagg cgttaacgcc acagcacttt
    77761 ttacgccttt ttcacactca gcgaggttgg attcactaat gccgtagatg ccataaacag
    77821 tacgctcttc accggtaggc ccctgtttaa aatctttcag ccagtcagca taacgcgcca
    77881 gactgctctg tacagggatc tgtaacttgt tatagcactt gatatagaca tccatttttt
    77941 cgtcgaaaac cgcttgttca tcgaccaccg gttgcgcagg tgtttcaggt ttagcttctg
    78001 cttttgcaac gggagcttca ggagctggtg cggcaggttg actattggca ggcgggggcg
    78061 tttctgtttc ggctttttta tcatcacaac ccgtcagacc gagggccatg atggcgacta
    78121 taatagcgga agataataaa ttccttttca tttaattgcc ttataaatat atagataaga
    78181 gagtgaacat aataaatgaa aaaagaaaac gccactacac gcgttttatt tataaata
//
LOCUS       DAZGSP010000006        76927 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZGSP010000006 DAZGSP010000000
VERSION     DAZGSP010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 76927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.006"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(246..1250)
                     /gene="trpS"
                     /locus_tag="ACHK74_000477"
     CDS             complement(246..1250)
                     /gene="trpS"
                     /locus_tag="ACHK74_000477"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFP0219361.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(1243..2001)
                     /gene="gph"
                     /locus_tag="ACHK74_000478"
     CDS             complement(1243..2001)
                     /gene="gph"
                     /locus_tag="ACHK74_000478"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFP0219362.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(1994..2671)
                     /gene="rpe"
                     /locus_tag="ACHK74_000479"
     CDS             complement(1994..2671)
                     /gene="rpe"
                     /locus_tag="ACHK74_000479"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP0219363.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(2689..3525)
                     /gene="dam"
                     /locus_tag="ACHK74_000480"
     CDS             complement(2689..3525)
                     /gene="dam"
                     /locus_tag="ACHK74_000480"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP0219364.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(3632..4918)
                     /gene="damX"
                     /locus_tag="ACHK74_000481"
     CDS             complement(3632..4918)
                     /gene="damX"
                     /locus_tag="ACHK74_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFP0219365.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(5010..6098)
                     /gene="aroB"
                     /locus_tag="ACHK74_000482"
     CDS             complement(5010..6098)
                     /gene="aroB"
                     /locus_tag="ACHK74_000482"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFP0219366.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(6155..6676)
                     /gene="aroK"
                     /locus_tag="ACHK74_000483"
     CDS             complement(6155..6676)
                     /gene="aroK"
                     /locus_tag="ACHK74_000483"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFP0219367.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(7077..8315)
                     /gene="hofQ"
                     /locus_tag="ACHK74_000484"
     CDS             complement(7077..8315)
                     /gene="hofQ"
                     /locus_tag="ACHK74_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFP0219368.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(8227..8631)
                     /gene="hofP"
                     /locus_tag="ACHK74_000485"
     CDS             complement(8227..8631)
                     /gene="hofP"
                     /locus_tag="ACHK74_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFP0219369.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGEHIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(8621..9061)
                     /gene="hofO"
                     /locus_tag="ACHK74_000486"
     CDS             complement(8621..9061)
                     /gene="hofO"
                     /locus_tag="ACHK74_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFP0219370.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(9045..9584)
                     /gene="hofN"
                     /locus_tag="ACHK74_000487"
     CDS             complement(9045..9584)
                     /gene="hofN"
                     /locus_tag="ACHK74_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFP0219371.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(9584..10363)
                     /gene="hofM"
                     /locus_tag="ACHK74_000488"
     CDS             complement(9584..10363)
                     /gene="hofM"
                     /locus_tag="ACHK74_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFP0219372.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQQFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            10483..13035
                     /gene="mrcA"
                     /locus_tag="ACHK74_000489"
     CDS             10483..13035
                     /gene="mrcA"
                     /locus_tag="ACHK74_000489"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFP0219373.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(13200..13760)
                     /gene="nudE"
                     /locus_tag="ACHK74_000490"
     CDS             complement(13200..13760)
                     /gene="nudE"
                     /locus_tag="ACHK74_000490"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFP0219374.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            14080..16215
                     /gene="igaA"
                     /locus_tag="ACHK74_000491"
     CDS             14080..16215
                     /gene="igaA"
                     /locus_tag="ACHK74_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFP0219375.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            16280..16948
                     /gene="yrfG"
                     /locus_tag="ACHK74_000492"
     CDS             16280..16948
                     /gene="yrfG"
                     /locus_tag="ACHK74_000492"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFP0219376.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            16959..17360
                     /gene="hslR"
                     /locus_tag="ACHK74_000493"
     CDS             16959..17360
                     /gene="hslR"
                     /locus_tag="ACHK74_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFP0219377.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            17385..18263
                     /gene="hslO"
                     /locus_tag="ACHK74_000494"
     CDS             17385..18263
                     /gene="hslO"
                     /locus_tag="ACHK74_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFP0219378.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(18398..20122)
                     /gene="yhgE"
                     /locus_tag="ACHK74_000495"
     CDS             complement(18398..20122)
                     /gene="yhgE"
                     /locus_tag="ACHK74_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFP0219379.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFNTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGVLAVLVLLVWSLGY
                     FVSIVWRKGQNPVVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TSDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            20501..22123
                     /gene="pckA"
                     /locus_tag="ACHK74_000496"
     CDS             20501..22123
                     /gene="pckA"
                     /locus_tag="ACHK74_000496"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFP0219380.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPIQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            22240..22557
                     /locus_tag="ACHK74_000497"
     CDS             22240..22557
                     /locus_tag="ACHK74_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0219381.1"
                     /translation="MFTFIELQGFSKRRQLLLPDDEFRIFQELLIQDPTAGDIIAGTG
                     SFRKIRWNRSGMGKRGGIRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSE
                     KLL"
     gene            22616..22912
                     /gene="nadS"
                     /locus_tag="ACHK74_000498"
     CDS             22616..22912
                     /gene="nadS"
                     /locus_tag="ACHK74_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFP0219382.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEETPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(22943..24295)
                     /gene="envZ"
                     /locus_tag="ACHK74_000499"
     CDS             complement(22943..24295)
                     /gene="envZ"
                     /locus_tag="ACHK74_000499"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFP0219383.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(24292..25011)
                     /gene="ompR"
                     /locus_tag="ACHK74_000500"
     CDS             complement(24292..25011)
                     /gene="ompR"
                     /locus_tag="ACHK74_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFP0219384.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            25239..25715
                     /gene="greB"
                     /locus_tag="ACHK74_000501"
     CDS             25239..25715
                     /gene="greB"
                     /locus_tag="ACHK74_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFP0219385.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            25812..28133
                     /gene="yhgF"
                     /locus_tag="ACHK74_000502"
     CDS             25812..28133
                     /gene="yhgF"
                     /locus_tag="ACHK74_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFP0219386.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQTAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMSKKR"
     gene            28571..28798
                     /gene="feoA"
                     /locus_tag="ACHK74_000503"
     CDS             28571..28798
                     /gene="feoA"
                     /locus_tag="ACHK74_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFP0219387.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            28815..31136
                     /gene="feoB"
                     /locus_tag="ACHK74_000504"
     CDS             28815..31136
                     /gene="feoB"
                     /locus_tag="ACHK74_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFP0219388.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALDRLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            31136..31372
                     /gene="feoC"
                     /locus_tag="ACHK74_000505"
     CDS             31136..31372
                     /gene="feoC"
                     /locus_tag="ACHK74_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFP0219389.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            31575..32453
                     /gene="rpnA"
                     /locus_tag="ACHK74_000506"
     CDS             31575..32453
                     /gene="rpnA"
                     /locus_tag="ACHK74_000506"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFP0219390.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(32482..33252)
                     /gene="bioH"
                     /locus_tag="ACHK74_000507"
     CDS             complement(32482..33252)
                     /gene="bioH"
                     /locus_tag="ACHK74_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFP0219391.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIVLTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            33290..33973
                     /gene="gntX"
                     /locus_tag="ACHK74_000508"
     CDS             33290..33973
                     /gene="gntX"
                     /locus_tag="ACHK74_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFP0219392.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            34032..34607
                     /gene="nfuA"
                     /locus_tag="ACHK74_000509"
     CDS             34032..34607
                     /gene="nfuA"
                     /locus_tag="ACHK74_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFP0219393.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            34968..36284
                     /gene="gntT"
                     /locus_tag="ACHK74_000510"
     CDS             34968..36284
                     /gene="gntT"
                     /locus_tag="ACHK74_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP0219394.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(36329..38413)
                     /gene="malQ"
                     /locus_tag="ACHK74_000511"
     CDS             complement(36329..38413)
                     /gene="malQ"
                     /locus_tag="ACHK74_000511"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFP0219395.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(38423..40816)
                     /gene="malP"
                     /locus_tag="ACHK74_000512"
     CDS             complement(38423..40816)
                     /gene="malP"
                     /locus_tag="ACHK74_000512"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFP0219396.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            41428..44133
                     /gene="malT"
                     /locus_tag="ACHK74_000513"
     CDS             41428..44133
                     /gene="malT"
                     /locus_tag="ACHK74_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFP0219397.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(44176..45192)
                     /gene="rtcA"
                     /locus_tag="ACHK74_000514"
     CDS             complement(44176..45192)
                     /gene="rtcA"
                     /locus_tag="ACHK74_000514"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFP0219398.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMCGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(45196..46422)
                     /gene="rtcB"
                     /locus_tag="ACHK74_000515"
     CDS             complement(45196..46422)
                     /gene="rtcB"
                     /locus_tag="ACHK74_000515"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFP0219399.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVTQKTVRQQQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            46611..48209
                     /gene="rtcR"
                     /locus_tag="ACHK74_000516"
     CDS             46611..48209
                     /gene="rtcR"
                     /locus_tag="ACHK74_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFP0219400.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRLQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(48191..48949)
                     /locus_tag="ACHK74_000517"
     CDS             complement(48191..48949)
                     /locus_tag="ACHK74_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFP0219401.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(48966..49796)
                     /gene="glpG"
                     /locus_tag="ACHK74_000518"
     CDS             complement(48966..49796)
                     /gene="glpG"
                     /locus_tag="ACHK74_000518"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFP0219402.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(49841..50167)
                     /gene="glpE"
                     /locus_tag="ACHK74_000519"
     CDS             complement(49841..50167)
                     /gene="glpE"
                     /locus_tag="ACHK74_000519"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFP0219403.1"
                     /translation="MDQFECINVAVAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            50357..51862
                     /gene="glpD"
                     /locus_tag="ACHK74_000520"
     CDS             50357..51862
                     /gene="glpD"
                     /locus_tag="ACHK74_000520"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFP0219404.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(51916..52521)
                     /locus_tag="ACHK74_000521"
     CDS             complement(51916..52521)
                     /locus_tag="ACHK74_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219405.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(52524..53291)
                     /locus_tag="ACHK74_000522"
     CDS             complement(52524..53291)
                     /locus_tag="ACHK74_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219406.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(53294..54004)
                     /locus_tag="ACHK74_000523"
     CDS             complement(53294..54004)
                     /locus_tag="ACHK74_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219407.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(54020..55528)
                     /locus_tag="ACHK74_000524"
     CDS             complement(54020..55528)
                     /locus_tag="ACHK74_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP0219408.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFHQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNDDKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(55653..58100)
                     /gene="glgP"
                     /locus_tag="ACHK74_000525"
     CDS             complement(55653..58100)
                     /gene="glgP"
                     /locus_tag="ACHK74_000525"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFP0219409.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(58119..59552)
                     /gene="glgA"
                     /locus_tag="ACHK74_000526"
     CDS             complement(58119..59552)
                     /gene="glgA"
                     /locus_tag="ACHK74_000526"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFP0219410.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(59552..60847)
                     /gene="glgC"
                     /locus_tag="ACHK74_000527"
     CDS             complement(59552..60847)
                     /gene="glgC"
                     /locus_tag="ACHK74_000527"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFP0219411.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(60865..62838)
                     /gene="glgX"
                     /locus_tag="ACHK74_000528"
     CDS             complement(60865..62838)
                     /gene="glgX"
                     /locus_tag="ACHK74_000528"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFP0219412.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMALSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(62835..65021)
                     /gene="glgB"
                     /locus_tag="ACHK74_000529"
     CDS             complement(62835..65021)
                     /gene="glgB"
                     /locus_tag="ACHK74_000529"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFP0219413.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(65294..66397)
                     /gene="asd"
                     /locus_tag="ACHK74_000530"
     CDS             complement(65294..66397)
                     /gene="asd"
                     /locus_tag="ACHK74_000530"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP0219414.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            66590..67183
                     /gene="yhgN"
                     /locus_tag="ACHK74_000531"
     CDS             66590..67183
                     /gene="yhgN"
                     /locus_tag="ACHK74_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFP0219415.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(67229..68524)
                     /locus_tag="ACHK74_000532"
     CDS             complement(67229..68524)
                     /locus_tag="ACHK74_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFP0219416.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEHKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWIITRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAIEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVPIR
                     LSDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAHTPVDFYQLFVAELAIFAKESIS
                     IERLSSCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(68431..70527)
                     /locus_tag="ACHK74_000533"
     CDS             complement(68431..70527)
                     /locus_tag="ACHK74_000533"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HFP0219417.1"
                     /translation="MEKHAAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDADLVAEWLRTNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKNELAD
                     VESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(70667..72007)
                     /gene="gntU"
                     /locus_tag="ACHK74_000534"
     CDS             complement(70667..72007)
                     /gene="gntU"
                     /locus_tag="ACHK74_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP0219418.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(72011..72538)
                     /gene="gntK"
                     /locus_tag="ACHK74_000535"
     CDS             complement(72011..72538)
                     /gene="gntK"
                     /locus_tag="ACHK74_000535"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP0219419.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(72677..73672)
                     /gene="gntR"
                     /locus_tag="ACHK74_000536"
     CDS             complement(72677..73672)
                     /gene="gntR"
                     /locus_tag="ACHK74_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFP0219420.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(73896..74591)
                     /gene="yhhW"
                     /locus_tag="ACHK74_000537"
     CDS             complement(73896..74591)
                     /gene="yhhW"
                     /locus_tag="ACHK74_000537"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFP0219421.1"
                     /translation="MIYFRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPNSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(74714..75751)
                     /gene="yhhX"
                     /locus_tag="ACHK74_000538"
     CDS             complement(74714..75751)
                     /gene="yhhX"
                     /locus_tag="ACHK74_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP0219422.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            76084..76572
                     /gene="yhhY"
                     /locus_tag="ACHK74_000539"
     CDS             76084..76572
                     /gene="yhhY"
                     /locus_tag="ACHK74_000539"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP0219423.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            76809..>76927
                     /locus_tag="ACHK74_000540"
     CDS             76809..>76927
                     /locus_tag="ACHK74_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFP0219424.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEI"
BASE COUNT        18733 a        20795 c        19224 g        18175 t
ORIGIN      
        1 gtttatatct tcattaaaat gaagataagt ggcaactgtg agatgtggaa acggcgaggc
       61 gcttcacatt ttttcggatt attgcaaatg aggataaaaa aaccgggttt ccccggtttc
      121 agagtgatga cgaagtgatt tattcgacaa tgcgagcaaa gggtaattcc cactggcgct
      181 tgcctgctcc cctcacccta accctctccc caaaggggcg aggggaccgt ccgagcgttt
      241 tgatattacg gcttcgccac aaaaccaatc gcttcgtaca ccgctttcag cgtacgggaa
      301 gcgtgtacac tggctttttc tgcgccgtct ttcatcacct gttgcaggaa ggcttcatcg
      361 ttgcggaaac ggtgatagcg ttcctgcaat tcagtcagca taccggaaac ggcatcagcc
      421 acttcacctt tcagatgacc atacatcttg ccttcgaact gtttttccag ttctgggatg
      481 ctctggcccg ttaccgcaga aaggatatcc agcaggttgg aaacgcccgc tttgttctgc
      541 acatcgtagc gaactaccgg cggctcgtca gaatcagtga ccgcgcgttt gattttcttc
      601 actaccgatt tcggatcttc cagcaggccg ataacgttat tgcggttatc gtcagacttg
      661 gacatcttct tggtcggctc cagcagcgac attacgcgcg cgccagattt cggaataaac
      721 ggctccggca ccttaaagat ctcgccatac agcgcgttga aacgctgagc gatatcgcgg
      781 ctcagttcca ggtgctgttt ctggtcttca cccaccggta ccagattagt ttgatacagc
      841 aggatgtccg cagccatcag caccggatag tcaaacagac cagcgttgat gttctcggca
      901 taacgcgcag atttatcttt aaactgcgtc atgcggctca gttcgccgaa gtaggtatag
      961 cagttcagtg cccagcctaa ctgcgcatgt tctggtacgt gggactgaac aaaaatggtg
     1021 cttttctcag gatcgatacc acaagccaga tacaaggcca gcgtatccaa cgtcgcttta
     1081 cgcagcttct gtgcatcctg gcgcacggtg atcgcgtgtt ggtcaacgat acagtaaatg
     1141 caatggtagt catcctgcat gtttacccac tgacgcagcg cacccatgta gttaccaatg
     1201 gtcaattcac ctgagggctg tgcgccacta aaaacgatgg gcttagtcat ttttcgattc
     1261 ctgattttcg ctatgcggaa gccctaatgc gggcagaagg tcatttatag actgataaat
     1321 tacatcaggc tggctgagat cgatagcctc gccgtagtta tatccgtagg ttaagccaac
     1381 tgatgggcaa cctgccgctt ttgccgcctg aatatcattg cgtgagtcgc cgacaaacag
     1441 catctgttgt ggggcaattc ccatccgctc agccaccagt aacagcgggt ccggatgcgg
     1501 ttttttgttt tgcacatcat cgccgccgat aaccacgcta aagtatttgg cgatatctaa
     1561 ggcttcgagc agcggcgcga cgaacggcgt cggtttgttg gtgaccaggc ctagcggcag
     1621 gcctttagcc tgcaacgcgc ccaacgtatc ggcaacgtgc gggaacaaaa acgtcccctc
     1681 ttcggcaacc tcgccatagt agcgatcgaa cagtttacgc agaatacgta cctgttcttc
     1741 tgccggaatg tcgtcatcaa cgggcggttt acccattgtt ttacgcagag tcgcacgttc
     1801 ctgacgcgcc caggtcaatg cgcgctccat cagaacatct gcgccgttac caatccaggt
     1861 aataacacgt tcttcacctg caacgggcaa ctccagcgca tacagcgcca tatctaccgc
     1921 agcagcaaga ccgggagcac tgtccaccag cgtaccgtcg agatcaaaag cgacgccgcg
     1981 aatatcttca aacttactca tgacttacct ttgccagttc actgcgcatt tcatcaatga
     2041 cttttttgta gtctggctgg tcgaagattg ccgaaccggc aacgaacata tccgcgcccg
     2101 ccgcggcgat ttcgccaatg ttgttcacct tcacgccacc gtccacttcc agacgaatgt
     2161 caaagccaga ctcgtcgata cgacgacgta cttcgcgcag tttatccagt gtttgaggaa
     2221 tgaaagactg accgccgaaa ccaggattga cggacatcag caggatcaca tccagcttat
     2281 ccatcacgta atccagatag ctcagaggtg tcgccgggtt aaataccaga cccgctttac
     2341 agccattttc tttaatcagt tgcagcgtgc ggtcaacatg ctcggaggct tctggatgaa
     2401 aggtaatgat gctggcacca gcggcagcga aatcaggcac aatgcgatcg acaggtttca
     2461 ccatcaggtg tacgtcgata ggggcggtaa tgccatagtt acgcaaggat ttcagcacca
     2521 ttggcccaat cgtcagattg ggaacatagt ggttatccat gacgtcaaaa tgcacgacat
     2581 cagcgccagc tgccagggct tttgcggtat cttcacccag gcgggcaaaa tccgccgaca
     2641 gaattgaggg ggcaatcaaa tactgtttca tccgcttctc cttgagaatt attttttcgc
     2701 gggtgaaacg actcctggtt tgtacaaagc cagcagttcg tccacctttt tacgtgtgcc
     2761 gccgttgctg cttatactgc gtcgaacttt gacgacatgc aattttgcgc gctgatacca
     2821 ctcacgcgtt aacatcgtat cgtgattgga gatcagcact ggaatatggc gatcaaccag
     2881 accttcggcg atctccgcca gatgcgcttg ttgttcaagc gtaaaactgt ttgtgtgata
     2941 cgccgtaaag ttggcggtcg cagacagcgg tgcataaggc ggatcgcaat agacgacgga
     3001 tgcatcatct gcgcgcgcca tgctatcggc gtaagactca caatagaaaa aggcattctg
     3061 cgctttttca gcgaagtgat acaactctgc ttccgggaaa tagggttttt tgtagcggcc
     3121 aaacggcacg ttaaactcac cgcgcagatt gtaacgacac aggccgttgt aaccgtagcg
     3181 gttcaaatat aaaaacagta ccgcccgacg gaacggatcc tggcttttgt tgaactcttc
     3241 gcggaactga tagtaaacct cggcgcaatt tgtttcggga acaaacagct cgcgtgcggc
     3301 ctgtacgtac tcatcagtac gcatcttcac aatgttatag agactgatca ggtcgctatt
     3361 gatatcggca aggatataac gagaaaagtc ggtgttgaga aacaccgacc cggcacctac
     3421 aaaaggctca accagacatt cgcccttggg caaatgccgt ttaatatcat caagcagggg
     3481 atacttgccc cctgcccact tcaaaaaagc gcgatttttc ttcatgctga ctaactaatt
     3541 acaccttctc cggctgtgga gaaagctccg acagcatcca gcgcttcagg caactcccgc
     3601 aacgataaac cattgcggga gataaccttg attacttcag atcggcctgt acctgacgca
     3661 gcggtttcgc ccacgggttt ttggcctgga catctgctgg caatgtagat accgcttttt
     3721 tcgcctcttc tttcgaagcg tacacgccag aaaccaggac ataccacggc tgaccattac
     3781 gcgtcgtttc atagacaacg tagtttttca gattctcttt cttcgcccaa ccgttcaggt
     3841 tgtcgtagtt agaggaactg ctcagctgca gagtgtaatg gctggacggt gccgatttca
     3901 acgaacccac attacctgcg gtcttccctc cagcggctgg tgttgccgtg gtttgcgccg
     3961 gggatgccgt ctgtactgga gccgtagtcg ccgtctcttt tggtgctggc gtagaagtcg
     4021 cagccggtgc cttcgtgctc gccacaggag cagctggttc agtacgcttc ggcgtctgtg
     4081 ctaccggctt cggttccgtt ttcacggttg cttgcggttt tttcggttca atcaccgcct
     4141 gctgacgagc tggacgcgta gtggccggac gttcagcagt ttgcgttttc gccgtgtcac
     4201 gcgatgcatt gccattgcgt actggcgcga cagttgctgg ttcagtcggc aatgtagaat
     4261 tgaccgccac attgttcaac tgttgctgat tttgtggctg ggtcagcgca ttgttcaggt
     4321 caccctgcac ttcaacacgt tgttgaccat ccgttgccgc cggggtttgc ccttgagtcg
     4381 gcgtagaaga gatcggtggc agagaaacat cctgctgagt attttccgca gacgtggttc
     4441 ccggcgctgg ctgcacccca ttcgcctgat cggtcgcatt gcctgcaaga tcaatactct
     4501 tctcgccaga cgcggtttga tcgctggaag aggtcgaggg ggcttttagc gcagaaccga
     4561 taccgatgat caacagcagt agaaccagaa tgccgacgcc catcatcata tactgacgag
     4621 aagcgggttt actggctgct tttttgcgct tacgcggacg acgctctacg cgctcttcat
     4681 ccacggtttc atcttcggat tcgtcaattt cttcttcgat ttccggttcc tcattgcgct
     4741 ctttttgcgc acgagtcgga cggcgatcgt cagtgtcatc aagttcaata tcatcaaaat
     4801 tgatctgcgg ttcgccacgt tcagtacgct cagaacgttc agaagattga cgagaacgac
     4861 cagtacgacg atcgctggga tcgggtttca gctcgtcttc tggtttgaat tcatccattt
     4921 aacaccccac taaaaggtta atgcttacca cgttgcaatt aacctgaagc taatagaccg
     4981 cttgataagc ggcctgacct ttcttgttgt tacgctgatt gacaatcggc aatggcgtta
     5041 agaacaagct cgtgcgaaac gccgctgcga acttcactct taccaattgc caacggaaga
     5101 attaagcgca tctctcccgc aaggactttc ttgtcacgca gcatatgcgg taaatacgcc
     5161 tgcgcggaca tttcgcgcgg cccattgacc ggtaacccag cccgcgtgag cagggttata
     5221 atacgctgcg tttcggcaga actaaactgc ccgagacgtt ccgacgtccg cgccgccatc
     5281 accatacccg cagcgaccgc ttcaccatgt aaccaattgc cataccccat ttcagcttca
     5341 atggcatgac caaaggtgtg tcccagattc agtaaagcac gtaacccggt ttcgcgctcg
     5401 tcggcggcga caacttctgc cttcagttca caacaacggc gaatacagta cgccattgcc
     5461 ggaccgtcca gacgcaacaa cgcatccaga ttctcttcca gccagttaaa aaacgcaccg
     5521 tcaagaataa tgccgtattt gatgacttct gccagccccg acgctaactc acgcggggga
     5581 agcgttttca gacagtcgag atccaccacc actgaagcag gctggtagaa cgcgccaatc
     5641 atgtttttac cgaggggatg gttgaccgca gttttgccgc caacggagga atcgacctgc
     5701 gacagtaacg tcgtcgggac ttgaatgaaa cgaacaccgc gctgataact cgccgccgcg
     5761 aagccggtca gatcgcccac tacgccgccg ccaagcgcca ccagcgtagt atcgcgacca
     5821 tgcggctttt gtaacaacgc cgtaaagacg gtatcgagta cagccaggct tttatactgc
     5881 tcgccgtcag ggaggataac gctatcgacg ttaacacccg cctgttcaag tacgccgcgg
     5941 accttatcga gatacagagg agccagggtt tcgttggtga ccaacatgac ctgctcgccc
     6001 gatttcagcg gtaagaatga agctggttca ttaaacaaac cagatgcgat ggtaattggg
     6061 taactacgtt ccccgagagt aacgacaatc ctctccataa cgcgacatcc accttaatta
     6121 ctgtacccgc agacgagtgt atataaagcc agaattagtt gctttccagc atgtgaataa
     6181 tctggtttgc aaccacttta gcgctttgat catcagtacg aatggtcacg tcggcaatct
     6241 cttcatacag cggattgcgt tcattggcca acgcttccag aacttcacgc ggcggtgttt
     6301 caacgtgcag caacgggcgt tttttatcac gctgcgtgcg tgcaagttgc ttttcgatgg
     6361 tcgtttcaag ataaacgaca acgccacgag cggaaagacg gttacgcgtt tcacgggatt
     6421 tcacagagcc gccgccagta gccagcacaa taccctgttt ctcggtcaac tcattgatga
     6481 ccttttcttc gcgatcgcgg aagccttctt cgccttctaa atcgaaaacc cagcccacat
     6541 cagctccggt tcgtttctca atctcttgat cggaatcgta aaattccata ttgagttgtt
     6601 gagctaactg gcgcccaata gtgctttttc cggcacccat aggcccaacc agaaagatat
     6661 tgcgtttctc tgccattttt tcggtactac taagactatt cgttaatgat aaacccgctt
     6721 cgctcagaga gcgccgcagg acatgaactg aaacctcata agatattgcg agagtcagac
     6781 tgaaaattat ctcaatactc cagcgggttt ggcaactgaa taaatcaccc atccctctgc
     6841 atatctggtc gctgcaagcg cgctgccttg ctaccaccgc tctggcgata aatcaccggg
     6901 taagattagc gtaaaaaaga cagcaaaatg ccgcctgaat gataaatcat catcatggaa
     6961 cgcaggcagc gaagagcacc aactctcaaa tcggtactcc ttgtatgcta aatacctgcg
     7021 cgcgtcaaat agatgaaaca cgttcagcat aaaaacatta ccgcttttac ggctgtttac
     7081 tcactggaaa ccagtcgtgg cgtgataaac accactaact cgcgtcgttc atcttctttt
     7141 ccgtcatgac gaaataattg cccgaaccag ggaatgtcgc caagcaacgg tacgctatcc
     7201 tgacccgatt tatttttacg ggtaaaaatg ccgcccagcg ccaacgtttc tccgcttttg
     7261 acctcgacct gcgtttcgat ctcctgctta tcaatcgcca gcacttcgcc atcggcctgc
     7321 tgtagcacct gccccggaac gttctggctg atgtgtaatt tcagccggat gcgacctttt
     7381 tgtaacaccg tgggcgtgac ctccatcccc aggacggcct ctttaaattc caccgacgtc
     7441 gcgccacttt ccccgctgga aacctgatat ggaatttcgc tcccctgttt aatgctggca
     7501 ggctgaagat gtgaggccag cagacgcgga ctggcgataa tatccagctg ctgtttttgt
     7561 tcgagcgcag aaagctcaag atccagcaag cgtccgttga tgcgcccaat gttaaaaccg
     7621 acatgcgttg tcgccgtcgc tacggagagg tcgctaccaa gcgtggtgac ttgcccaacg
     7681 ccaccagcgt gttgcgcatc ggccagcgtc catttcacgc ctaactcacg caaacttttt
     7741 tcattaatgg tgacaatatg cgccgacagc tcaacctgcc cgaccggcag atccatttgc
     7801 gctacccact gttcaagcgc gcttaacgcc gttttgttat ctcgtagcaa aaggcgattg
     7861 gtgcgtttat cgacggtcat actccctttg gcactcagta gcttctcccc cgctttcgcc
     7921 agttctcccg cgtcggcgta ttgcagggtt atactgcgat tttccagcgg cagatttgcc
     7981 tgcgcccgcg cctgctccgc ctcctggcgg gcgatattgt tattctgcca ggcaatggaa
     8041 tgcactgaga gaatgttgtc ttcctgccgc gttatcagtc cggcgctttt cactacagtt
     8101 tgtagtgcct gcttccaggg aacatctgtt agatgtaacg acaccgtacc gctgacgtct
     8161 ggcgacacga ccaggttcaa cttctcctgt tcagccagcg cctgcaacac ctgagctacc
     8221 ggaacgtcat ccaccatcag cgtcactttt tgcggctttg ccgcctgtac gccgggtatc
     8281 agcatcaaca gtagtgcggc tatccattgc ttcatttgta tctccttgcc gttgccacaa
     8341 ccattgtggc ggttcgcagt ttgtcccggt tcccagcgtt agtgcgtctg gcgtcagctg
     8401 taaaattgtc cagccgtttt ccagcacatc gttttgctgc acccgtcgcc atttcttttg
     8461 cccgtctttt attacaccga tgatgtgctc gcctcgccct accatcccct gatagcgcca
     8521 ctggctaagt tcgctaatcc ggcatagatc ttccggcggt ttaaaagggt cacgcatacc
     8581 ggttaaaagg cacaatgcaa tacctgccaa caaccagcgt ttaaccctca tgcggtgtct
     8641 ccagttgtag cgtgaacaaa agatcatcac cttccacgct taacgaaaaa cggctcacgc
     8701 tgacgttgcg ctctgccagc cgtgtaaatg ccgacggcac tgcttcccac agcgttttca
     8761 acgccaactc acctccctgc gcggatggat gccaggaaac cagttgcgcg ccggataact
     8821 gaaaatccag aggcgaaaag ggcagcgttt tttcctcgct gaagggagtg gtgtctacca
     8881 ggcgatacag tgcggcccac tgctggtgat gacttgcccg tagtcgaatt aatgcgtcgc
     8941 gctcttcatg gtgtgtcgat gatagaaaaa tgagcgtaac taacatcagc aaccagaatg
     9001 cccagcaaag ctggcggagg cggggtgatg tggcgaacca ccagtcaaag aacatgttca
     9061 tcgctaacct tccttgttaa ctgatactca aattgccagc gtccctgcgc atcctgctgc
     9121 gtggctcccc gctgattgag atgaaaagac gcatcctggc ggagtgacgt ttctagtgcg
     9181 tttaacgcgg taatgcttgt tgtcagcccc ttgatctcca gcgttccctg ctgccagctt
     9241 atcgttgtca gccaggcgtg ctcgggtaaa agcgccgcca gtgcttccag cgcagattgc
     9301 cagtcgcggg taaattgtcg ctggcgctgc cgttgcgaac gctgttcgcg taactgctgt
     9361 tgctccagca aacgtggctt cgttatctgt aagctgcggg cgagttgttg ttccgcctga
     9421 agcaaaactg cgtctatgcg cgcttcggcg ctgcctgtca gacgcagtat tagcgttatc
     9481 ccgacggcca gcagcagagg cgcaacgaac atcagcaacc agaaacgcag aaaagcggtc
     9541 cggcgttgct gtcgccaggg caaaaaatta attggcgggt tcatcagtac gccttcccaa
     9601 gcgccagccc cagcgcgatg gcaaagtctc cacctggcgg cggtagcggc ggctgacgaa
     9661 cagaaacggc ctcccaggga tcaaatccgc cttcgccaca tatcgcgacg ctttcgggat
     9721 caacggataa cgctgccgcc agctccttcg cgctagtcat ccccaccgcc agtttgcgcc
     9781 cccagcgaca gcgtgtcgcc cacagccact gttcgttatc acgccaggcc agacattgct
     9841 gatgagaagg taaaaaaggc aggaattgct gtaatgcact ggcatccggg gtgatcgcgc
     9901 tcacatgaac acgcaacctt tctgccagag taagtagcgt tgccagctct ttgctttgcg
     9961 cggcagtcac gttataggcg gggctgagtg agtcttcgct gtaatcgaag cgcagggagt
    10021 ccggatccat atccagctcg cgggccatcg tccctgacag ccaggccgtt tgctcccgct
    10081 caccaaggga catcgacggg cgcggaaatg accgctgtaa tgtgcgactg gcgggaaacg
    10141 ccaacataat gtgatgacgc tgcggcagtt cgcgactcca aggtaacaac gttttagcca
    10201 gctgctgcgc atcaacaatc cgcccatctt tgataatgtc gttctccagc ggcaaccgcc
    10261 accagcgttg caaaaagcat tcttttgcgc cccgcacgat cgcaaccgct accgcttctt
    10321 gctgttgtaa atgcaaacca atttgccaga tcttaaatgc cattgtgatg atctccttat
    10381 cacccgtcac tctgacgggt atatcaatgc gtctggcttg cctttatact accgcgcgtt
    10441 tgtttataaa ctgcccaaat gaaactaaat gggaaatttc cagtgaagtt cgtaaagtat
    10501 tttttgatcc ttgcagtctg ttgcattctg ctgggagcag gctcgattta tggcctatac
    10561 cgctacatcg agccacaact gccggatgtg gcgacattaa aagatgttcg cctgcaaatt
    10621 ccgatgcaga tttacagcgc cgatggcgag ctgattgctc aatacggtga gaaacgtcgt
    10681 attccggtta cgttggatca aatcccaccg gagatggtga aagcctttat cgcgacggaa
    10741 gacagccgct tctacgagca tcacggcgtt gacccggtgg ggatcttccg tgcggcaagt
    10801 gtggcgctgt tctccggcca cgcgtcacaa ggggcgagta ccattaccca gcagctggcg
    10861 agaaacttct tcctcagtcc agaacgcacg ctgatgcgta agattaagga agtcttcctc
    10921 gcgattcgca ttgaacagtt gctgacgaaa gacgagatcc tcgagcttta tctgaacaag
    10981 atttacctcg gttaccgcgc ctatggtgtc ggtgctgcgg cacaagtcta tttcggtaaa
    11041 acggtcgacc aactgacgct gaacgaaatg gctgtgatag ccgggctgcc gaaagcgcct
    11101 tccaccttta atccgctcta ctcaatggat cgtgccgtcg cgcggcgtaa cgtcgtactg
    11161 tcgcggatgc tggatgaagg gtatatcacc caacaacagt tcgatcagac acgcactgag
    11221 gcgattaacg ctaactatca cgcgccggag attgctttct ctgcgccgta cctgagcgaa
    11281 atggtgcgcc aggagatgta taaccgttat ggcgaaagtg cctatgaaga cggttatcgc
    11341 atttacacca ccatcacccg caaagtgcag caggccgcac agcaggcggt acgtaataac
    11401 gtgctggact acgacatgcg ccacggctat cgcggcccgg caaatgtgct gtggaaagtg
    11461 ggcgagtcgg cgtgggataa caacaagatt accgatacgc tgaaggcgct gccaacctat
    11521 ggtccgctgc tgcctgccgc agtcaccagc gccaatcctc agcaagcgac ggcgatgctg
    11581 gcggacgggt cgaccgtcgc attgagtatg gaaggcgttc gctgggcgcg tccttaccgt
    11641 tcggatactc agcaaggacc gacgccgcgt aaagtgaccg atgttctgca aacgggtcag
    11701 caaatctggg ttcgtcaggt tggcgatgca tggtggctgg cacaagtgcc ggaagtgaac
    11761 tcggcgctgg tgtcgatcaa tccgcaaaac ggtgccgtta tggcgctggt cggtggcttt
    11821 gatttcaatc agagcaagtt taaccgcgcc acccaggcac tgcgtcaggt gggttccaac
    11881 atcaaaccgt tcctctacac cgcggcgatg gataaaggtc tgacgctggc aagtatgttg
    11941 aacgatgtgc caatttctcg ctgggatgca ggtgccggtt ctgactggca gccgaagaac
    12001 tcaccaccgc agtatgctgg tccaattcgc ttacgtcagg ggctgggtca gtcgaaaaac
    12061 gtggtgatgg tacgcgcaat gcgggcgatg ggcgtcgact acgctgcaga atatctgcaa
    12121 cgcttcggct tcccggcgca aaacattgtc cacaccgaat cgctggcgct gggttcagcg
    12181 tccttcaccc caatgcaggt ggcgcgcggc tacgcggtca tggcgaacgg tggcttcctg
    12241 gtggacccgt ggtttatcag caaaattgaa aacgatcagg gcggcgtgat tttcgaagcg
    12301 aaaccgaaag tagcctgccc ggaatgcgat attccggtga tttacggtga tacgcagaaa
    12361 tcgaacgtgc tggaaaataa cgatgttgaa gatgtcgcta tctcccgcga gcagcagaat
    12421 gtttctgtac caatgccgca gctggagcag gcaaatcagg cgttagtggc gaagactggc
    12481 gcgcaggagt acgcaccgca cgtcatcaac actccgctgg cattcctgat taagagtgct
    12541 ttgaacacca atatctttgg tgaaccaggc tggcagggta ccggctggcg tgcaggtcgt
    12601 gatttgcagc gtcgcgatat cggcgggaaa accggtacca ctaacagttc gaaagatgcg
    12661 tggttctcgg gttacggtcc gggcgttgtg acctcggtct ggattggctt tgatgatcac
    12721 cgtcgtaatc tcggtcatac aacggcttcc ggagcgatta aagatcagat ctcaggttac
    12781 gaaggcggtg ccaagagtgc ccagcctgca tgggacgctt atatgaaagc cgttcttgaa
    12841 ggtgtgccgg agcagccgct gacgccgcct ccgggtattg tgacggtgaa tatcgatcgc
    12901 agcaccgggc agttagctaa tggtggcaac agccgcgaag agtatttcat cgaaggtacg
    12961 cagccgacac aacaggcggt gcacgaggtg ggaacgacca ttatcgataa tggtgaggca
    13021 caggaattgt tctgattaaa aaggcgcttc ggcgcctttt cagttggctg acaaagtgca
    13081 tttgtttatg ccggatgcgg gtgaacgcgt tatccggcta acaaaatcgt gaaaaatcaa
    13141 tatattgcag gaaccccata agcctgataa gcgttgtgca tcaggcaaac ttcacgcatt
    13201 tacactcgcc cctgcccttt caaccattcg cgcacgagga acagcgcgct gacattacgc
    13261 gcttcgttga agtcagggtc ttccagcaaa tccatcatat gcgccagcgg ccagcgcacc
    13321 tgtggtagcg gctctggctc atcgccttcc agcgattccg ggtagagatc ttgcgcgacc
    13381 acgatattca ttttgctgga aaagtaagac ggtgccatgc tgagcttctt caaaaaagtc
    13441 agatcgttcg caccaaatcc aacctcttct tttagctcgc ggttagcggc ttcgtagacg
    13501 ctttcacccg gatcaattaa tcctttcgaa aaacctaatt cgtaggattc agttcccact
    13561 gcgtattcgc ggatcaggat caggtgatcg tccacaatcg gcacaatcat cactgcttcc
    13621 cggttggttg gacgcattcg ttcataaaca cgccgcacgc cattgctgaa ctccagatcc
    13681 acgctctcga cggtaaacag tcgggaacgg gctacagttt caacattcag aatggtgggt
    13741 ttttgtaatg atttgctcat cgtgggatct atgctgtgaa atcagcggtt attgtgcgat
    13801 atcggacact ctttcggcaa tgtgaattgc atgttattta catttatgta acttaataaa
    13861 taatcgtcct caaatcaaat taaaagtcaa taggttgaaa taactccagg aatttgctga
    13921 tattccgcct tcagagggtt tgctatgatc agcggttact gtgatgtgct taatgatgct
    13981 caagttaaac tccacgcttg ccgatagcca accgcagaat catgtattgt gtctggtgcg
    14041 actgaccacg cctgacagac taagtaagat ggggaaagca tgagcaccat tgtgattttt
    14101 ttagctgctt tgctggcctg ctcactactt gcgggatggc tgataaaagt gcgatccaga
    14161 cggcgtcagc tgccctggac caacgccttc gcggatgcgc aaacgcgtaa actcacacct
    14221 gaagaacgta gcgccgttga aaattatctt gagagcctga cgcaggtatt acaggtgcct
    14281 ggcccaacgg gagctagcgc ggcaccgatc tctctggcgc tgaatgccga aagcaacaac
    14341 gtcatgatgc tgacacacgc tatcacgcgt tacggcatct ctaccgacga tccgaataaa
    14401 tggcgttact acctcgattc ggtagaagtc cacctgcccc ctttctggga acaatacatc
    14461 aacgatgaga ataccgttga actgatttat accgattcgc tgccgctggt tatttcactc
    14521 aacggtcata cgctgcagga gtacatgcag gaaacgcgcg gctatgcctt gcaacctgtt
    14581 ccgtccacgc aggcgtcgat tcgcggggaa gagagtgagc aaatcgagct actcaatatt
    14641 cgcaaagaaa cgcatgaaga atatgcgctg agtcgtccgc gcgggctgcg tgaagcgttg
    14701 ctgatcgtcg cctccttcct gatgttcttt ttctgcctga ttaccccgga tgtatttgtt
    14761 ccgtggctgg caggcggcgc attactgctg ctgggcgcag gtctgtgggg gctattcgcg
    14821 cccccggcaa aatcctccct gcgagaaatt cattgtctgc gcggtacacc ccgtcgttgg
    14881 ggattgtttg gcgaaaacga tcaggaacag atcaacaata tttcactcgg tattatcgac
    14941 ctggtctatc ccgcacactg gcagccatac attgctcagg atctcggtca acaaaccgat
    15001 attgatatct atctcgaccg ccacgtagtg cgtcaggggc gatatctttc gctgcatgat
    15061 gaagtaaaaa actttccgtt acagcactgg ctgcgcagta cgattatcgc tgcgggctcg
    15121 ctgctggtgc tgtttatgct gttattctgg atcccgctgg atatgccgct gaaattcact
    15181 ctctcatgga tgaaaggcgc gcagaccatt gaagccacca gcgtaaaaca actggctgac
    15241 gctggcgtgc gggtaggcga tacattgcgt attagcggta cgggaatgtg taatattcgc
    15301 acttccggaa cctggagcgc gaaaaccaat tcaccttttt taccgtttga ctgctcgcag
    15361 atcatctgga atgacgcccg ctcattacca ttaccagaat ctgaactggt caacaaagcg
    15421 acggcattga ctgaagcagt taatcgccag ctgcacccta aaccggaaga tgaatctcgc
    15481 gtcagtgcct cattacgttc agcgattcaa aaatccggca tggtattgct tgatgatttt
    15541 ggcgacattg tactgaagac agcggattta tgttctgcca aagatgactg tgtgcgactg
    15601 aaaaatgcgc tggtcaatct cggcaacagt aaagactggg acgcgctggt aaaacgcgcc
    15661 aacgccggga agctcgatgg cgtgaatgtg ttattacgcc cggtgagtgc agaatcgctg
    15721 gataacctgg tggcaacctc caccgcgccg tttatcacgc atgaaacggc gcgagcggca
    15781 caatcactaa acagtccggc ccccggcgga ttcctgattg tcagcgacga aggcagcgat
    15841 tttgttgatc agccctggcc ttcggcatca ctttacgact acccgccgca agaacagtgg
    15901 aacgctttcc agaaactggc acaaatgctg atgcatacgc cgtttaacgc cgaaggtatc
    15961 gtcacaaaaa tcttcactga cgccaatggt acgcagcata ttggccttca tccgatcccg
    16021 gatcgttccg gcctgtggcg ctatctcagc accacattgc tgctactgac gatgctgggt
    16081 agcgccattt acaatggcgt acaggcctgg cgtcgttacc agcgtcatcg cactcgcatg
    16141 atggagattc aggcctatta tgaaagctgc ctgaacccgc aactgatcac cccttcagaa
    16201 agccttatcg aataacacgt ttgcgcggca ggttatgcta ccctgtcgcg caaattgctt
    16261 cactctggag atttccctca tgcatatcaa cattgcctgg caggacgtag ataccgttct
    16321 gctggatatg gacggcacgt tgctcgacct cgccttcgat aactatttct ggcaaaagct
    16381 ggtgcctgaa acatggggcg cgaaaaacgg ggttacgcca caggaagcga tggaatatat
    16441 gcgccagcaa tatcacgacg tacagcatac gctaaactgg tactgtcttg attactggag
    16501 tgagcaactg ggtctggata tctgtgcgat gaccaccgag atgggaccgc gtgccgtact
    16561 gcgtgaagat accattccgt ttcttgaggc actgaaagcc agcggtaagc agcgaatttt
    16621 gctcaccaat gcgcatccgc acaacctggc ggtaaaactt gagcataccg gtctggacgc
    16681 acaccttgat ttattacttt ccacccacac atttggttat ccgaaagagg atcagcggtt
    16741 atggcatgcg gtggccgaag ctacgggtct gaaagctgaa agaacgctgt ttattgatga
    16801 cagcgaagcg attctcgatg ctgccgcgca atttggtatt cgttactgcc tcggcgtgac
    16861 taatcctgat tccgggattg ccgagaaaca gtatcaacgc catccgtcac tgaatgacta
    16921 ccgccgcctg atcccctcgc taatgtgaag gagacgccat gaaagaaaaa cctgctgttg
    16981 aggttcgact ggataaatgg ctatgggctg cccgttttta taaaacccgc gcgctggccc
    17041 gtgaaatgat tgaaggcggt aaggtgcatt acaacgggca gcgcagcaag ccgagcaaaa
    17101 tcgtcgagct gaatgccacg ctcactctgc gccagggaaa tgacgaacgc acggtgattg
    17161 taaaggcgat tactgaacag cgtcgccccg ccagcgaggc agccttgctg tatgaagaga
    17221 ctgcggaaag tgtagagaaa cgcgaaaaaa tggcgatggc acgtaaactt aatgccttaa
    17281 ccatgccgca cccggaccga cgcccggaca aaaaagagcg ccgcgacctg ttacgattta
    17341 aacacggcga cagtgaataa ctgtcacctg caagagagat gattatgccg caacatgacc
    17401 aattacatcg ctatctgttt gaaaattttg ccgttcgcgg cgaactggta accgtttcgg
    17461 aaaccctgca acagatcctt gagaaccacg attatccgca gcccgttaaa aacgtgctgg
    17521 cagaactgct ggttgcgacc agcctgttaa ccgctacgct gaagtttgat ggtgatatca
    17581 ccgtacagct gcaaggcgac ggtccgatga atctggcggt tattaacggt aacaataacc
    17641 agcaaatgcg cggtgtggcg cgcgtgcagg gcgaaattcc agaaaatgcc gacctgaaaa
    17701 cgctggtcgg caatggttac gtggtgatca ccattacccc gagcgaaggc gaacgctatc
    17761 agggcgtggt tggtctggaa ggtgataccc tggcggcctg cctggaagat tactttatgc
    17821 gttctgaaca gctgccgacg cgcctgttta ttcgcaccgg cgacgtagac ggcaaaccgg
    17881 ctgcaggcgg tatgttgttg caggtaatgc ctgcgcaaaa tgcccagcag gacgactttg
    17941 accacctggc gacgctaacc gaaaccatca aaaccgaaga actgctgacc ttaccggcaa
    18001 acgaagtgtt gtggcgtttg tatcacgaag aagaggtgac ggtttacgat ccgcaggatg
    18061 tggagttcaa atgcacctgc tcgcgtgaac gttgcgccga tgcgctgaaa acgctgcctg
    18121 atgaagaagt tgatagcatc ctggcggaag atggcgaaat tgacatgcat tgtgattact
    18181 gcggtaacca ctatctgttc aatgcgatgg atattgctga aatccgcaac aacgcgtctc
    18241 cggcagatcc gcaagttcat taatgagtat gtccggcaga gagggctctg ccggatttta
    18301 cgacgtaatg gcctgatgtg ctgcgcttac caggcctgtg aaataaatcg caaattattg
    18361 ttggtcagat acagcgtcgt atccgttaaa agagttgtca cttacttaag tttatttcca
    18421 gcgtttcacc atctaccgtc agatcgcgcc acgctttcgg cctcgtcccc agttttccat
    18481 ccttcgcagc tgtgagtaat ttttctttcg ttatttcgtt cggaagtaaa ctcatatcaa
    18541 gcgcgtccca ttcttttctg tctgagtcaa agccatagac aatgactctg tcatcattaa
    18601 aagcaaacag gatccgctcc gcctgaccat cactattcag atcttgctcg accaggacgc
    18661 aggcgtcttt ttcaatacag gtcatcacgt tatagcgatc ctggattaag gccgaccaga
    18721 atgtcgcatc cggtttaaca gaaccagggg caattaacac attttcggct aatacttttt
    18781 ccgatacctg ttcctgaaga tgttgctctc catctaacgc catcagcaga tcccgcgcgc
    18841 gtttcgggtc tttcataaac ccggcatcgc ttttcagcga ctcaagcgca gcacgtccat
    18901 agcgaccgct ctgctcgagc atgtagatag ttacctggtc tgacgtgttt ttgccgctct
    18961 gataacgcgc catatggctg ttcacgctaa tgcgcatact gtccagcacc ggcgaattaa
    19021 gaagcaccag tatcaccaac accaataacg aaaccgcaag gttcactttg ccctgaagaa
    19081 caacgggatt ttgcccttta cgccagacga tgctgacaaa ataccccagc gaccacacca
    19141 gtaacaccag caccgccagc acgccctgca agcggtcgac agtccagccg tactgagcga
    19201 cccgcagcca taacgcccag gcggcgacga acacatacag cggagcaacc agcaaagcgg
    19261 ttttaatcag gcaacgcaac ggccctgtcc agggaagtga cgctttttgc ggatcgcgga
    19321 caatagccat taagatcaat tgcaaaaagg ccagcgttaa cagcaacccg gcggcggaga
    19381 tgtggcgaga aatcgcgctc aggcccgtaa acggcagagt gatgataaac atcagggtta
    19441 gcaatgatac taacggcagc aaccccgtgg cgattaacgt gaacaacttt tgaatagagt
    19501 cgattaaacg tgactgtgtt cgcgcgagga tcaccgccag cgcggtaacc agacctaacg
    19561 tgagataaat aaaccagtcg gttgcaaaaa agagcgtatt gaaaaacgtg atgccaacaa
    19621 gtttaaacaa ctcactccac agtaaaagca ccagccacgt caagccattg gcgagaaaaa
    19681 tcaccagtaa tataagtacg ttatgccata ctgactggta aaaatagcga tagcggctac
    19741 tgtcattgcg gatgcgaagg ctttgttgta tccacggtag caacaacatt gccatcagca
    19801 gcagatagca accaaaatcc caaagcgcct tttcagctct ccaggggttc atgccatcag
    19861 tttgccactt cagccagccg ctcatcccca gcgtggcaat aaacaccagc gccagccatc
    19921 cccaaaggcg cttctgttta aaagagatca cggagaagag taaaacggat gaaaacgcaa
    19981 cggtcgcagg cacgccataa acaatccaac tgtgattttt tccggcaagc caggcgatca
    20041 acaggtagca aaccagcccc tgaagtaatc cggtagcaat catcccccag cgtgttgccg
    20101 gtgaaagttc aacgttatcc atgttggtta tccagaatca aaaggtgggt taattatcgc
    20161 atccgggcag tagtattttg cttttttcag aaaataatca aaaaaagtta gcgtggtgaa
    20221 tcgatacttt accgattgaa tttgcatcaa tttcattaag gaatgcgatt ccactcacaa
    20281 tattcccgcc atacaaacca agatttaacc ttttgagaac attttccaca cctaaaatgc
    20341 tatttctgcg ataatagcaa ccgtttcgtg acaggaatca cggagttttt tgtcaaatat
    20401 gaatttctcc agatacgtaa atctatgagc cttgtcgcgg ttaacacccc caaaaagact
    20461 ttactattca ggcaatacat attggctaag gagcagtgaa atgcgcgtta acaatggttt
    20521 gaccccgcaa gaactcgagg cttatggtat cagtgatgta catgatatcg tttacaaccc
    20581 aagctacgac ctgctgtatc aggaagagct cgatccgagc ctgacaggtt atgagcgcgg
    20641 ggtgttaact aatctgggtg ccgttgccgt cgataccggg atcttcaccg gtcgttcacc
    20701 aaaagataag tatatcgtcc gtgacgatac cactcgcgat actttctggt gggcagacaa
    20761 aggcaaaggt aagaacgaca acaaacctct ctctccggaa acctggcaac atctgaaagg
    20821 cctggtgacc aaacagcttt ccggcaaacg tctgttcgtt gtcgacgcct tctgtggtgc
    20881 gaacccggat actcgtcttt ccgtccgttt catcaccgaa gtggcctggc aggcgcattt
    20941 tgtcaaaaac atgtttattc gcccgagcga tgaagagctg gcaggtttca aaccagactt
    21001 tatcgttatg aacggcgcga agtgcactaa cccgcagtgg aaagaacagg gtctgaattc
    21061 cgaaaacttc gtggcgttta acctgaccga gcgcatgcag ctgattggcg gcacctggta
    21121 cggcggcgaa atgaagaaag ggatgttctc gatgatgaac tacctgctgc cgctgaaagg
    21181 tatcgcttct atgcactgct ccgccaacgt tggcgagaaa ggcgatgttg cggtgttctt
    21241 cggcctttcc ggcaccggta aaaccaccct ttccaccgac ccgaaacgtc gcctgattgg
    21301 cgatgacgaa cacggctggg acgatgacgg cgtgtttaac ttcgaaggcg gctgctacgc
    21361 aaaaactatc aagctgtcga aagaagcgga acctgaaatc tacaacgcta tccgtcgtga
    21421 tgcgttgctg gaaaacgtca ccgtgcgtga agatggcact atcgactttg atgatggttc
    21481 aaaaaccgag aacacccgcg tttcttatcc gatctatcac atcgataaca ttgttaagcc
    21541 ggtttccaaa gcgggccacg cgactaaggt tatcttcctg actgctgatg ctttcggcgt
    21601 gttgccgccg gtttctcgcc tgactgccga tcaaacccag tatcacttcc tctctggctt
    21661 caccgccaaa ctggccggta ctgagcgtgg catcaccgaa ccgacgccaa ccttctccgc
    21721 ttgcttcggc gcggcgttcc tgtcgctgca cccaattcag tacgcagaag tgctggtaaa
    21781 acgtatgcaa gcggcaggtg cgcaggcgta tctggttaac accggctgga acggcactgg
    21841 aaaacgtatc tcgattaaag atacccgcgc tattatcgac gccatcctca acggttcgct
    21901 ggataacgca gaaactttca ctctgccgat gtttaacctg gcgatcccaa ccgaactgcc
    21961 gggcgtagac acgaagattc tcgatccgcg taacacctac gcttccccgg aacagtggca
    22021 ggaaaaagcc gaaaccctgg cgaaactgtt tatcgacaac ttcgataaat acaccgacac
    22081 ccctgcgggt gccgcgctgg tagcggctgg tccgaagctg taatcagctg atatctcatt
    22141 gaccccttat ccaaacggat aaggggtttt tttatatcta attagctacc aatatgtaca
    22201 cggtacactt ataattatca atagaccgca ggatgcatta tgttcacctt tattgaacta
    22261 caaggattta gcaaacgccg ccagttattg ctacctgatg atgagttccg gatttttcag
    22321 gaactactca ttcaagatcc cactgccggt gacattatcg caggcacagg cagttttaga
    22381 aaaattcgct ggaaccgttc tggaatgggg aaacggggtg gcattcggat aatttattac
    22441 aacgtaacac gcaatggccg aatttatctg gcgttgattt atcctaaaaa tgaacaggat
    22501 gatttaacag aagaacaaag aaaggcattg aaactattgt ccgagaagct gctttaatgt
    22561 tgaagctcca caaattccac catggtcgaa atgccaacgg agacattgaa aacagatgaa
    22621 agatgaatta tttgctgatc ttcttgccag cgcagaagaa atggtacgca tcgaaaaggg
    22681 tgaagaaacg cctgaaccag agcacgtcca tacttttagc gagatcgatg taaaagcgat
    22741 ccgtgaagca acgggtttac gtcagcaaga ttttgctgtc gccgttgggg taagttatga
    22801 cctggtcaaa agctgggaaa ccaaacgccg tcaacctacc ggagcacctc gaaaattgct
    22861 cctgcttttg caagcgaatc ctttcattat caatcaattg aaaacaatct aaaaacggga
    22921 ggcaccttcg cctcccgttt atttaccctt cttttgtcgt gccctgcgcc cgcgttaccg
    22981 gcactggcag ccaggcgcga atggaaagcc cgccccgctc gctggtgcca agctccagca
    23041 tcccgttatg gttatccacg atacgctgca caattgccag ccctaatccc gtgccgctaa
    23101 tggtgcgcgc actgtcgccg cggacaaacg gctggaacag gtgcttacgt tgttccggcg
    23161 caattcccgg accgtcatct tccacctgga accaggcgcg attcggctcc gtcccgctgc
    23221 tgactttgat ccagccattg ccgtaacggg cggcgttgac caccatattc gccaccgcgc
    23281 gtttgatcga cagcgggtgc attttcactt caatgctgcc ggggtaaagc gcggtttcaa
    23341 tttcccgctc atagccactt tcggcagcaa taacctcacc gagtactgcg ttgagatccg
    23401 ccatttccat cggcatctcc tgtccggtgc gcaggtagtc gataaactgc tcaatgatgg
    23461 cattgcactc ctcgatatct ttattgatcg attctgccag atagccatcc tgctcgctca
    23521 tcatctcggt cgccaggcga atacgcgtca gcggtgtacg caagtcgtga cttacccccg
    23581 ccatcagcag cgtgcggtca tccgccagtt gcttaacacc agccgccata tggttaaagg
    23641 cgcgggtaac ggaacgcacc tcggacgcgc catactcacg cagcggcggc ggaataatcc
    23701 ctttaccgac ctgcaaagca gcatgttcga gatcgaccaa cggtcggttc tggatacgaa
    23761 taaacagcca cgccccgcct atcgccaata gcataatcgc cagcgtatag cggaacagcg
    23821 gagagaaatc gccctgatga atttcggtca gcggcacgcg tacccagata ttgggcgaca
    23881 gccaggtttt cagccagacg acaggcgaac ttttgttgac ctcaacgcgc acttccgtcg
    23941 ggccgcccag ttgctgcgcc atctgatggc ttaagaattc atagtgttgc gcccaacgca
    24001 gacctgcctc ttcggcagcc tcgttggagt agagagagat ccccagctca cggtagatct
    24061 cccgacggaa agcgggaggc acaaccaact gcgtgccgtc ctccagttgc agtttgtcgg
    24121 tcatcaacat acgcacttcg tacgcgagga ctttattaaa ctgctggagg ctcggcaaaa
    24181 tcgcgaagtt cagcaccacc agataagtcg tcaccaggct ggcgaacagc aaggtgacga
    24241 tgagcaataa cgtacgggca aatgaacttc gtggcgagaa gcgcaatcgc ctcatgcttt
    24301 agagccgtcc ggtacaaaga cgtagcccag accccagacg gtctgaatgt aacgcggatg
    24361 cgctggatct tcttccacca tgcggcgcag acgcgaaatc tgcacgtcga tggagcgttc
    24421 cattgcggaa tattcacgac cacgggcaag gttcatcagc ttatcgcggg agagcggctc
    24481 acgcggatgg ctgaccagtg ccttcagtac cgcaaactca ccgctggtga gcggcatcgg
    24541 ctcgtcttcg cggaacattt cgcgcgtacc gaggttaagt ttgaacttac cgaaagcaat
    24601 taccgcctct tcctgtgacg gtgcgcctgg tagctcgttc gcctgacgac gcagcaccgc
    24661 acggatacgg gccagcagtt cacgcgggtt aaacggtttt ggaatgtaat cgtcagcgcc
    24721 aatctccagg ccaacgatac gatccacttc ttcccctttc gccgtcacca taatgatcgg
    24781 catcgggttg ctctgactac gaagacgtcg gcaaatcgac aagccatctt caccaggtaa
    24841 cattaaatcc agtaccataa gatggaaaga ttcacgagtc agcaggcgat ccatctgttc
    24901 tgcattagcg acgcttcgaa cctggaagcc ttgttcggtg agataacgtt ccagcagcgc
    24961 acgcaggcgc atgtcgtcat cgaccaccag aatcttgtag ttctcttgca ttgtttgtac
    25021 tcccaaaggt tcgcaacaat ttgtaagcgt gtattcttaa aaaagctcac gttcgtcacc
    25081 agctaaatct ggtatgaatt tcagcctaaa ttgttacaaa gcatattaaa cagcagctta
    25141 agtatacaat ttattcggcg aaacattatt gattctgttg atatgatcac gttataccca
    25201 atgtgcgcat tatcaaacag acaaagggaa tcaacgagat gaaaacgccc ctggttaccc
    25261 gggaagggta tgaaaaactc aaacaagagc ttaattatct ctggcgtgaa gaacgcccgg
    25321 aggtcacaaa aaaggtgacc tgggccgcaa gtctgggcga ccgcagcgaa aatgctgact
    25381 atcagtataa taaaaagcgt ctgcgtgaaa tcgaccgtcg cgtgcgctat ctcactaaat
    25441 gcctggaaaa tctcaaaatc gtcgattact cccctcagca ggaaggcaaa gtcttttttg
    25501 gcgcgtgggt ggagattgaa aacgacgatg gcgtgactca ccgtttccgt attgtcggct
    25561 acgatgaaat ttttggccgt aaagattaca tctctatcga ttccccgatg gcccgcgcat
    25621 tgctgaaaaa agaagtcggc gatctggcgg tggtgaatac ccctgccggg gaagcgagct
    25681 ggtatgttaa tgctatcgag tacgtgaaac cgtaaggaag agtcttaacc tcctgccgat
    25741 ggctggcatt tttgccagcc agtccgtata actatcccct gatttttgat ccgaaaagat
    25801 gaactcaaac catgatgaat gattcgttct gccgcattat tgcgggtgaa attcaggcgc
    25861 gcccggaaca ggttgacgct gccgttcgcc tgcttgacga agggaatacc gtgccgttta
    25921 tcgcacgtta tcgtaaggaa atcaccggcg gtctggatga cacgcagctg cgtaatctgg
    25981 aaacgcgtct gagctatctg cgcgagctgg aagagagacg tcaggcgatc ctcaaatcca
    26041 tttccgagca aggcaaactc accgatgatc tggcgaaggc catcaacgcc accctaagca
    26101 aaaccgaact cgaagacctc tacctgccct acaaacctaa acgccgcacc cgcgggcaaa
    26161 tcgccattga agcagggctt gagccgttgg ctgacctgct gtggagcgat ccgtcacaca
    26221 cgccagaagt cgccgctgca caatatgttg atgccgataa aggcgtggca gataccaaag
    26281 ccgcgctgga tggcgcgcgc tatatcctga tggaacggtt tgccgaagat gccgcgctgc
    26341 tggcgaaagt gcgtgattat ctgtggaaga acgcgcattt ggtttctacg gtggtgagcg
    26401 gtaaagaaga ggaaggggcg aaattccgcg actatttcga tcatcacgaa ccgttgtcca
    26461 cggtgccttc tcaccgcgcg ctggcgatgt tccgtgggcg taacgaaggc gtactccagc
    26521 tttcgctgaa tgccgatccg cagttcgaag agccgccaaa agagagctat tgcgagcaaa
    26581 tcatcatgga tcatctcggc ctgcgcctga acaatgctcc ggcggatagc tggcgcaaag
    26641 gcgtggtgag ctggacctgg cgcatcaagg tgctgatgca tctggaaacc gaactgatgg
    26701 gcaccgtgcg cgaacgcgcg gaagatgaag caatcaacgt ctttgcccgt aacctgcacg
    26761 atctgctgat ggcggcccct gccggactgc gtgccacgat gggcctcgat ccgggtctgc
    26821 gtaccggggt aaaagtagcg gtggtcgatg ccactggcaa actggtggcg accgatacca
    26881 tttacccgca caccggacaa acagcaaaag cagcgatgac cgttgctgcc ttgtgtgaaa
    26941 agcataacgt tgaactggtg gcgatcggca acggtacagc ttcccgcgaa accgaacgtt
    27001 tctatctcga cgtgcagaag cagttcccga aagtgaccgc gcagaaagtg atcgtcagcg
    27061 aagctggcgc gtcggtttac tcggcttccg agctggcagc gcaggagttc ccggatctcg
    27121 acgtttcgct gcgtggtgcg gtatctatcg cccgccgttt gcaggatccg ctggcggagc
    27181 tggtgaaaat cgatccgaaa tctatcggcg taggtcagta tcagcatgac gtcagccaga
    27241 cgcaactggc ccgcaaactg gatgcagtag tagaagactg cgtaaacgcc gttggcgtcg
    27301 atctcaacac cgcttctgtt ccgctattaa cccgcgtggc gggcctgacg cgcatgatgg
    27361 cgcaaaacat cgtggcctgg cgcgatgaga acggccagtt caagaaccgt cagcaactgc
    27421 tgaaagtcag ccgtctgggg ccgaaagcct tcgagcagtg cgcgggcttc ctgcgcatta
    27481 accacggtga taacccgctg gacgcctcta ccgttcaccc ggaagcctat ccggtggtgg
    27541 aacgcattct ggcagcaaca cagcaggcac tgaaagatct gatgggtaac agcagcgaac
    27601 tgcgtaacct gaaagcgtct gactttactg atgaaaaatt cggtgtgccg acagtaactg
    27661 acatcatcaa agagctggaa aaaccgggtc gcgatccgcg tccggaattt aaaaccgctc
    27721 agtttgccga tggcgtcgag acaatgaacg acctgcaacc gggtatgatc ctcgaaggcg
    27781 ctgtcaccaa cgtcaccaac tttggcgcgt ttgtcgatat tggcgtgcat caggacggcc
    27841 tggttcacat ctcttcattg tcgaacaagt ttgtggaaga tccgcatacc gtggtgaaag
    27901 cgggcgacat tgtgaaggtg aaagtgctgg aagtggatct tcagcgtaaa cgtatcgccc
    27961 tgaccatgcg tctggacgag cagcctggcg aaaccaacgc ccgtcgcggc ggcggtaatg
    28021 aacgcccgca aaacaaccgc ccggcagcca aaccacgcgg tcgtgaagcg cagcctgccg
    28081 gtaatagcgc gatgatggat gcgctggcgg cggcaatgag caaaaaacgt taaacgccct
    28141 gattgacccc taaacctgcg cgataaggtg tccacgccgc atctggcaac gaacgccttg
    28201 tctgacataa aagtgccgga gaatatctcc ggcattttta ttccacagcc aaactcacaa
    28261 tatattccgg caacatttat cacttcatta acaactgaaa ccttaattaa acattagcca
    28321 gtcctggtaa ttcgctattc gaattatatt ttcgctgcga tataaccttg agccacatca
    28381 acattgagtc agattattat tcaaaccaac attcgcacac attttaagta ttgctgatag
    28441 aaaccattct cattatcatt gtgctgttga ttatttaatc tctccttcgt tggcaaatca
    28501 tctggtctca tgtcgctgtc aaacgcccca tgaggtagtt atccagttaa tgagaaacaa
    28561 gtaggcacct atgcaataca ctccagatac tgcgtggaaa atcactggct tttcccgtga
    28621 aatcagcccg gcatatcgcc aaaaactgct ttctcttggc atgttacctg gctcctcttt
    28681 taatgtggtg cgcgtcgctc cactcggcga ccccattcat atcgaaaccc gtcgtgtgag
    28741 cctggtatta cgcaaaaaag atctggcctt attagaagtg gaagcggttt cctgttaata
    28801 cggtgataac aacaatgaaa aaattaacca taggcttaat tggtaatcca aattctggca
    28861 agacaacctt atttaaccag ctcactggcg cacgtcagcg tgtaggtaac tgggctggcg
    28921 ttaccgtcga acgtaaagaa gggcaattct ccaccaccga tcatcaggtc acgctggtgg
    28981 acctgcccgg cacctattct ctgaccacca tctcatcaca gacctcgctc gatgagcaaa
    29041 tcgcctgtca ctacattttg agtggcgacg ccgacctgct gattaacgtg gtggatgcgt
    29101 ctaaccttga gcgtaacctg tacctgacgc tacaactgct ggaactcggc attccctgca
    29161 tcgtggcact gaacatgctc gacattgccg agaagcaaaa tattcgtatt gaaattgatg
    29221 ctctgtcggc gcgtcttggc tgcccagtga tcccgctggt ttcaacccgt ggtcgcggta
    29281 ttgaagcgct caagctggcg attgatcgct ataaagctaa cgagaatgtg gaactggtgc
    29341 attacgcaca gccgctactc aacgaagcag attcactggc aaaagtgatg ccttccgaca
    29401 tcccgctgaa acaacgtcgc tggctgggcc tgcaaatgct ggaaggcgat atctacagcc
    29461 gcgcctacgc cggtgaagcg tcgcagcatc tggatgccgc cctcgaccgt ctgcgtaatg
    29521 agatggacga tccggcgctg cacattgccg atgcgcgtta ccagtgcatt gctgccatct
    29581 gtgatgtggt aagcaacacc ctgacggcag aacccagccg tttcaccact gcggtagata
    29641 aaatcgtgct caaccgtttc ctcggtctgc cgattttcct ctttgtgatg tacctgatgt
    29701 tcctgctggc tatcaacatc ggcggggcgt tacagccgct gtttgacgtc ggctccgtgg
    29761 cgctatttgt gcatggtatt caatggattg gctacacgct ccacttcccg gactggctga
    29821 ctatcttcct cgcccagggc ctgggtggcg gcattaacac cgtgctgcca ctggtgccgc
    29881 agattggcat gatgtacctg ttcctctcct tccttgagga ctccgggtat atggcgcgtg
    29941 cggcgtttgt gatggaccgt ctgatgcagg cgctgggctt gccggggaaa tcctttgtgc
    30001 cgttgatcgt cggtttcggt tgtaacgtac cgtcggtaat gggtgcacgt acgcttgatg
    30061 caccgcgtga acgtctgatg accatcatga tggcaccgtt tatgtcctgc ggcgcgcgtc
    30121 tggctatctt cgcagtattc gcggctgcct tcttcgggca gaacggtgcg ctggcggtct
    30181 tctcgctgta tatgctgggt attgtgatgg cggtgctgac tggcctgatg ctcaagtaca
    30241 ccatcatgcg cggtgaagcg acgccgtttg tcatggagtt gccggtctat catgtaccac
    30301 acgttaaaag cctgattatc cagacctggc agcgtctgaa aggcttcgtt ctgcgtgctg
    30361 gtaaagtgat catcatcgtc agcattttcc tgagcgcttt caacagcttc tcgctgagcg
    30421 ggaaaatcgt cgataacatc aacgactcgg cgctggcttc cgtcagccgg gtgatcaccc
    30481 cggtcttcaa gccgattggc gtacatgaag ataactggca ggcaacggtt ggcctgttta
    30541 cgggcgcaat ggcgaaagaa gtggtggtgg gtacgctcaa caccctctac accgcagaaa
    30601 atattcagga cgaagagttc aatccggcgg aattcaacct cggtgaagag ctgttcagtg
    30661 cggtagatga aacctggcag agcctgaaag acaccttcag ccttagcgta ctgatgaacc
    30721 ccattgaagc tagcaaaggc gacggcgaaa tgggtaccgg ggcgatgggc gtgatggatc
    30781 agaaattcgg tagcgcagct gccgcataca gctacctgat tttcgtcctg ctgtatgtac
    30841 cgtgtatctc ggtgatgggg gctatcgccc gtgaatcaag ccgtggctgg atgggcttct
    30901 ccatcctgtg ggggctgaat atcgcttact cactggcaac attgttctat caggtcgcca
    30961 gctacagtca gcatccaact tacagcctgg tgtgcattct ggcggttatc ctgtttaaca
    31021 tcgtggttat cggtctgctg cgccgcgcgc gtagccgggt ggatatcgaa ctgctggcaa
    31081 cccgcaagtc ggtaagcagt tgctgcgcag ccagcaccac cggtgattgc cattaatggc
    31141 ttcacttatt caggtgcgcg atttgctggc gttacggggc cgtatggaag cggcccagat
    31201 aagccagaca ttgaacactc cacagccaat gattaacgcc atgctgcaac aactggaaag
    31261 tatgggcaaa gccgtgcgga ttcaggaaga acctgacggc tgcctctctg gcagttgtaa
    31321 aagctgcccg gaaggaaaag cctgtctgcg cgagtggtgg gcgctgcgtt aaccttactc
    31381 catcgcctgt tttggaaagc ccggtatgcg tctgcatccg ggcttttttg cgtgcggctt
    31441 tccataaaaa tgcaactctt gcagcacggc gtaagttcct ttgaaagcat ctcgcaggga
    31501 tgaaaactcg ctaatacaca ggagtgaagt ggcgcgtaga gtcgcggcat tcaaacaaca
    31561 ggtgaaggaa cgccatgagc aaaaagcaga gttccacccc acacgatgcg ctgttcaaac
    31621 tctttttacg ccaaccggac acggctcgtg attttcttgc gtttcattta ccggcaccca
    31681 ttcacgcgct ttgtgatatg aaaaccctca agctggagtc gagcagcttt attgatgacg
    31741 atctgcgtga aagctattcc gatgtgctgt ggtcggtgaa aacggaacaa ggaccaggat
    31801 acatctattg tctgattgaa catcaaagca cctcaaacaa actgatcgca tttcgcatga
    31861 tgcgttacgc tattgccgca atgcaaaatc accttgatgc tggatacaaa acgttgccga
    31921 tggtggtgct attgttgttt taccacggta ttgaaagccc ctatccctat tcgctgtgtt
    31981 ggctggattg tttcgccgat cccaaactgg caaggcagct ttatgcctcc gcatttccgc
    32041 tgattgatgt caccgtcatg cctgatgatg aaatcatgca gcaccgacgt atggcgctgc
    32101 tggagttaat tcaaaaacat attcgtcaac gcgacctgat ggggctggta gagcaaatgg
    32161 cctgcttatt aagtagtgga tacgctaatg acagacaaat caaagggctg tttaattaca
    32221 tactgcaaac cggcgacgcg gtacgtttta acgattttat cgacggcgtt gccgaacgtt
    32281 caccgaaaca caaggagagt ttaatgacaa ttgcggaaag attgcggcag gagggggaac
    32341 aatccaaagc cctgcatata gccaaaataa tgcttgaatc cggagtccct cttacagaca
    32401 tcatgcgctt taccggggtg tcagaagaag agttggctgc ggcgagccag taaatttctg
    32461 tctcgccatt tcaaaagttg cctacaccct ctgcttcaac gccaccagca ggtgacaaaa
    32521 ctcgaccgga tgcgaaataa acggcgcatg ggccgctttg gcgaagatat atgattcgct
    32581 gtgaggccaa agtttatcca gcatcggcac cactttgcgc ggcaccagac cgtcgagata
    32641 gccatacaat cgcaaaaacg gcatggacac gttttgcagc ggctgacgga gatcgaccgt
    32701 tttcaggatt tccagcccgc cattaagcac gtcaacctcc ggcatcggta acgccagaac
    32761 ggttttcttc aacgcccgcg catcctggcg cgccgtttca gtccccatgg tttgtaacgc
    32821 caggaaccgc tccactgtac gctgaaaatc atcactgagt tgctgctgaa atcccgccag
    32881 cacgtccggt tttatccccg gccactcgtc acgagcacta aaacaaggtg acgacgccac
    32941 ggtgaccagc gcctgaacac gctcgggatg ggttaaaaca atttggcttg ccaccagccc
    33001 gcccagactc cagcctaacc aaatggcttt atcaggtgcc tgttgcagca cggcttcggc
    33061 catatcagca agtgacagcg caccaaatcc ccggctacgc ccgaagccgg gcaggtcaac
    33121 aaggtgcagc gtaaaatgcg agctaagttc ctcgtcaatg caacgccaca cttcggcatt
    33181 cagtccccat ccgtgcagca gcacaagatg aacattcccc tgacctttgg tctgccacca
    33241 gatgttattc atccgctatt gttctctttt gacttacaag gatgaacata tgctaacagt
    33301 accgggatta tgctggctat gccgaatgcc actggcgtta ggtcattggg ggatttgttc
    33361 ggtctgttca cgcgccaccc gcacagataa aacgttatgc ccacaatgtg gattacccgc
    33421 cacacactcc catcttccct gcggtcgctg cctgcaaaaa ccgccgccct ggcaaagact
    33481 ggtcacggtt gccgactatg cgccgccgtt aagtccgctt atccaccagc ttaaattttc
    33541 ccggcgcagt gaaatcgcca gcgccctgtc acgtctgtta ctattggaag tcttacacgc
    33601 tcgtcgcacc accgggttgc aattgccgga tcgcatcatc agcgttccgt tatggcagcg
    33661 gcgtcactgg cgtcggggat ttaatcagag cgatttgctg tgtcagccgt tatcacgctg
    33721 gttgcactgc caatgggata gcgaagccgt cacacgtaca cgggccactg cgacccagca
    33781 ttttctcagt gcccggctgc gcaagcgcaa cctgaaaaat gcctttcgtc ttgaattgcc
    33841 cgtgcaaggt cgccatatgg tgattgtgga tgatgtcgtt accaccggaa gtaccgtcgc
    33901 agagattgcg cagttgcttt tacgcaatgg tgcggcgact gtccaggtct ggtgcctttg
    33961 tcgaaccttg tagagcctcg atgatgggcg tattataacc aactaaaata gtcaactatt
    34021 aggccattac tatgatccgt atttccgatg ctgcacaagc gcactttgcc aaactgctgg
    34081 caaatcagga agaagggaca caaatccgcg tatttgtgat taaccctggc acgcctaacg
    34141 ctgaatgtgg tgtttcttat tgtccgccgg atgccgtgga agccaccgac actgccctga
    34201 aatttgacct gctgaccgcg tatgttgatg agttaagcgc tccttacctg gaagatgcag
    34261 agatcgattt tgttaccgac cagttgggtt ctcagctaac actgaaagcg cctaacgcca
    34321 aaatgcgtaa agtggcagac gatgcaccac tgatggagcg cgtggagtat atgctgcagt
    34381 cgcagatcaa cccacagctt gctggtcacg gtggccgcgt ttcactgatg gaaatcaccg
    34441 aagacggtta cgccattctg caatttggcg gcggctgtaa cggttgttcc atggtcgatg
    34501 tgacgctgaa agaagggatc gagaagcagc tgctaaacga attcccggag ctgaaaggtg
    34561 tgcgcgatct caccgaacac cagcgcggcg aacactccta ctactaagtt atcccctctt
    34621 ttgtagattg cccgatgcga cgctaaagcg tcatttcggg cctacgttga acatgtgcca
    34681 gacgttggca cagcattgct ttggcaaatc cctcccttcg tttccccgcg ataatatgac
    34741 caacctctca taatttaaat ttaccccgct ctggtgattc tcaaacgcca gatgttaccc
    34801 gtatcattca catgggtacc aaacatactc ctgacatctg actacaataa ttagttttag
    34861 tgggtatcag tcgtggtgcc gcaatatctc tgttcccgat tgggataatt agagtttgtc
    34921 gtcagaaaat tgacgttacc cataacaaat gaaaggccag gtaaatcatg ccattagtca
    34981 ttgttgctat cggtgtaatc ttgttgttgc tcctgatgat ccgcttcaaa atgaacggct
    35041 tcatcgctct cgtcctcgtg gcgcttgctg ttggattaat gcaaggaatg ccgctggata
    35101 aagttattgg ctccatcaaa gccggtgtcg gcgggacgct cggtagcctt gccctgatca
    35161 tgggttttgg cgcaatgctg ggcaaaatgc tggcagactg cggtggcgca caacgtatcg
    35221 ccaccacgct gattgccaaa tttggtaaaa aacacatcca gtgggcggtg gtactgaccg
    35281 gttttaccgt tggttttgcc ctgttctatg aagtgggctt tgtgctgatg ctgccgctgg
    35341 tgtttaccat cgcggcttct gcgaatattc cactgttgta tgttggtgta ccaatggcag
    35401 ctgcactgtc tgtgacccac ggcttcctgc caccgcatcc gggtccgact gcgattgcca
    35461 ccattttcaa tgccgatatg ggtaaaaccc tgctgtacgg tactattctg gcaatcccga
    35521 ccgtgattct cgccggtccg gtttacgctc gcgtgttgaa aggtatcgat aagccaattc
    35581 cggaaggtct ctacagcgcg aaaaccttca gcgaagaaga gatgccgagc tttggcgtca
    35641 gcgtctggac ctctctggtg ccggtagtac tgatggcgat gcgtgcgatt gccgaaatga
    35701 tcctgccgaa aggtcacgct ttcctgccgg tagcggagtt cctcggtgac ccggtaatgg
    35761 ctacgctgat tgccgtgctg attgccatgt tcacctttgg tctgaaccgt ggtcgttcaa
    35821 tggatcagat taacgacacg ctggtttctt ccatcaaaat cattgcgatg atgctgttga
    35881 tcatcggtgg tggcggtgcg ttcaagcagg tgctggtaga cagcggcgtg gacaaataca
    35941 ttgcttccat gatgcacgaa accaacattt ctccgctgct gatggcctgg tcgattgctg
    36001 ccgtactgcg tatcgcgctg ggttccgcaa ccgttgcggc aatcactgcg ggtggtatcg
    36061 cggcaccgct gattgcaacg acgggtgtta gcccggagct gatggttatt gcggtgggtt
    36121 ccggtagtgt gattttctct catgtgaacg atccgggctt ctggctgttc aaagagtact
    36181 ttaacctgac tatcggcgag accatcaaat cctggtcgat gctggaaacc attatctcgg
    36241 tgtgcggtct ggtaggctgt ctgctgctga atatggtgat ttgataacac tgactgccgg
    36301 atgcatcgca tccggcatca accgcactct acttcttctt cgctgcagct ctgcgccgtc
    36361 tgtccaaatc cttcagcaac ttgttcacgc catcatcggc aaacatcgac tcaagcgttg
    36421 cggaaagctt gcgtcgccag tttttatact ggtaactggt gccaggaata ttcaccggtt
    36481 cggccatatc cagccagtct tccggctgta gtcctaacag agcactgtta ctgtcggcaa
    36541 tgtagcgctg caaaccacgg ttcagcgtcg gcgtcatcga catcaacgat gccttatgcc
    36601 cggcacgttt tggcagacaa ccatatttat gcagtgcatc cagcagccct tgcttcgcca
    36661 gttcgcgatc ctgatacaga ccgcgcagta ccacttcatc cggatacagc cccagggttt
    36721 tgcccaacgt tagatccccg ctctcccagt aaccgcgcag cgtaggcagg tcatgtgtcg
    36781 ccgcaaccgc catcgactgc tccggatacg ctttcggtgc acggaacgtc ttctcgtggt
    36841 cgttttcgaa atagagcact ttgtaggagt acacaccgct gctgcgcagc ttaccgacaa
    36901 tctctaccgg tacggtaccg agatcttcac caatcaccat acagcgatga cgtttacttt
    36961 cgagtgccag aatcgagagc agatcatcca ccggatagtg aacatacgcg ccctgatctg
    37021 ccgtctcgcc atacggtatc caccacaaac gcagcatcga catcacatgg tcaattcgta
    37081 atgcgccgca gttttgcata ttggcacgca acagctcgat aaacggttca taggcacgcg
    37141 cggtgatgat atgcgggtcc attggcggta atccccagtt ctgccccaac gggccgagga
    37201 tatccggcgg cgcgccaacc gacgctttca ggcaatatag ttcacgatca caccaggttt
    37261 ccgccccacc ttccgctacg ccaaccgcca gatcacgata caagccaatc ggcatttcat
    37321 agccctggct tatctcccag caggcggcaa actggctgta agccagccac tgcaaccaga
    37381 gataaaaatc gacgtcatca cgatgttctt cgcagaactg acgcacttct ggtgaatcca
    37441 cgttctgata catctctggc catgcaggcc agccccagcg catttcgtcc tctttcactt
    37501 gctgggcatg tagcgcatca aaggctgcct gccagaacag gctgtcgccc tgctctgcaa
    37561 caaactggcg aaacgcggtc atctgctcat catcacgttg cgcgaaacct ttccacgcca
    37621 ttcgtaatgc tgtcattttt agggcggtaa ccgtggagta atcgacccag tcggcatcgc
    37681 gcgcctgttg cagcgtctgt tgcgtggtcg gcaactgcca ccaggcctga gcctcttcgc
    37741 taagatggaa atcttcaacg gcgttaacgt cgatataaat cacattcagc caacggcgag
    37801 aagacgggct gtatgggctg gcactctccg gatttgccgg atagagcgca tgaatcgggt
    37861 tcaggccgat gaacgacccg ccacgttttg ccacatccac cagcatcgct ttgagatcgc
    37921 caaaatcccc aataccccag tttttttccg atcgcagcgt ataaagctga acgcaggcac
    37981 cccacagctt ttgtttattc agcaacgcct gcggttcgta acagcgtttc ggggcgacaa
    38041 tcacccggca atgcgcgcgc tggtcgtcct gggtgagtgt cagcgtgtga taaccttccg
    38101 gcagcttcgt cggtagattg aacgctttgc cccccgttac atggcctttg tactgtgttc
    38161 cttcttcggt ggtcagcagc cagctatatt cgccgctgcc ctccaccacc atcggcattt
    38221 ttttgccgct ggtataaacc atgacattcg ggactggcgt taccgccact ttcgtggcgg
    38281 tacgttgatg catcgcgtca agcaaacgcc gtttggtttc ggcgctaatc gactgcggtt
    38341 taccgtgggc attgatgtaa ttggggctaa tccccgccgc cagcgcggca ttatccagac
    38401 gtttgctttc catcgagctt ccttagcgtt ttgcctgcca gatacgagcc tgataatcgc
    38461 gaatagagcg atccgagcta aacataccgc agcgggcggt attgaggatc gccgcgcgag
    38521 tccaggcctc ctggtcgcgg tacagcacat ccacctgctt ttgtgcctca acataggctg
    38581 cgaaatccgc catcaccaga tacggatcgc cgccttgttt gccgatactg tgcagcatct
    38641 ggtcgaaggc atgcttatcg ccgtcgctgt atttaccgct ttccagctct ttcaatactg
    38701 cgtccagcac cttatctttc ttccgccatt tcaccgggtc gtagcctttg gccagaattg
    38761 ccttcacttg ttccacggta tgaccaaaaa taaagatatt ttcttcaccg actttctcgg
    38821 cgatttcaac gttcgcacca tccagcgtac cgacagtaag cgcaccattg agcgccagtt
    38881 tcatattgcc ggtaccggaa gcttctttac ctgcagtcga aatttgttcg gagatatccg
    38941 ccgccgggat cagtttttcc gccgccgaaa cgcaataatc cggcaggaac accaccttca
    39001 acttatcgcc aaccagtgga tcgttgttga tcacgtcagc cactttgttg atcgcaaaga
    39061 taatattctt cgccaggtag tagcccggtg ccgctttcgc gccgaagagg aagacgcgcg
    39121 gtacgcgatc agcctgcggg ttttcacgaa tttctttgta caacgccaga atatgcagca
    39181 gattcaggtg ctggcgtttg tactcatgca gacgtttgat ctgaatatcg aaaatcgcct
    39241 gtggattgat ctcaatacca gtacgaactt tcacaaactc cgccagacgg actttattcg
    39301 cctgcttgat ctcgcgatat tgctgacgga atttcgcatc atcagcgaat ttttccagat
    39361 tgatcagctg atcgagatcg ttagcccact ctttttgcag tgatttatcc aacagagccg
    39421 ccagtgcagg gttgcactgt ttgatccagc gacgtggggt aataccgttg gtgacgttat
    39481 ggaatttgtt cggccatagc tggtgatatt ccgggaacag atctttcacc accagatccg
    39541 agtgcagcgc cgcaacaccg ttcaccgcga aaccgccaac cacacacagg ttcgccatat
    39601 gcacttgttt atcgtgcacc accgccagtt tggcccacac tttttcatcg cccggccagg
    39661 ttttctccac cagcgtttta aagcgagtat taatttcgtt aataatctgc atatggcgcg
    39721 gcagtaagcc tttcaccagt ttcacatccc agcgttccag cgcttctggc atcagggtat
    39781 ggttggtgta agcgaaagtt ttgctggtaa tggcccaggc gtcatcccag ctcatctggt
    39841 gctcatcgat cagcacgcgc agcagttctg gaatcgcgat agttgggtgg gtatcgttca
    39901 gctgaataac ttcgtaatcc gccagttcgt gcagtttacg ccccgccaga tgatggcgac
    39961 gcaaaatatc cgctaccgaa caggcacact ggaagtactg ctgcatcaag cgcagctttt
    40021 taccggcagt atggttgtcg tttggataga gaactttggt cagtttttcc gcattgatgc
    40081 cctgctgttc ggcacgcagg aaatcaccgt cgttaaattt agtcagatca aacggatgcg
    40141 cgtgcgtcgc ctgccacaga cgcagcggct gcgccacgcc attacgatag ccgacaacgg
    40201 ggagatccca cgcttgaccg gtaatggtaa actccggctc ccagcgtccg tctttcgtca
    40261 ctttaccgcc aatccctacc tgcacatcca gtgcttcgtt gtggcggaac cacgggtagt
    40321 tactgcgatg ccagtcatcc ggcgcttcaa cctgtttgcc atcgacaaaa gactggcgga
    40381 acaaaccata ttgatagttc agaccgtaac ccgtcgcaga ctgaccgaca gttgccattg
    40441 agtcgaggaa gcacgccgcc agacgtccca gaccaccgtt acccagcgcc gggtcgatct
    40501 cttcttccag caggtccgtc agattgatgt cataagcctt caacgaatcc tgtacatcct
    40561 gataccagcc gagattcaac aggttgttgc ccgtcaggcg accaatcaaa aactccattg
    40621 agatgtagtt aacatgtcgc tgattcgcca ccggcttggc gaatggctga gcacgcagca
    40681 tttcggccag tgcttcactc actgctagcc accactggcg aggagtcatt tcagccgcag
    40741 aatttaagcc ataacgctgc cactgacgtg aaagcgcttc ctgaaattgc ttatcgttaa
    40801 aaataggttg tgacatagga gttccacttt tcttagattt tcaacacaac gttatcgcta
    40861 gtttgccagg ctcgatgttg accttcctca tcctgcgggg gattaggcag ggaggagttg
    40921 cggggatgag caaggaaatg tgatctcaac cacttaaagc tagtgcaaac cacaggatta
    40981 gcagcaaatc aatgcaatac agcgcagaaa atctgtatct aagtgcaaaa aatggccgtt
    41041 gcgtattttc aaaaagcgga aggtaactct ataaattaag taaaggagtg aaacagtttc
    41101 ataagtaaaa tatccagtgt gctccatctc attcttaata gatttattaa gatcatcttt
    41161 ttagatggca ctttcatcag gaatgaagaa gaaacccttg cttaaatgaa tctgatgaac
    41221 ataagggaaa ccagtattca cgctggatca gcgtcgtttt aggtgagttg ttaataaaga
    41281 tttggaattg tgacacagtg caaattcaga cacataaaaa aacgtcatcg cttgcattag
    41341 aaaggtttct ggccgacctt ataaccatta attacgaagc gcaaaaaaaa taatatttcc
    41401 tcattttcca cagtgaagtg attaactatg ctgattccgt caaaactaag tcgtccggtt
    41461 cgactcgacc ataccgtggt tcgtgagcgc ctgctggcta aactttccgg cgcgaacaac
    41521 ttccggctgg cgctgatcac gagtcctgcg ggctacggaa agaccaccct catttcccag
    41581 tgggcggcag gcaaaaacga tatcggctgg tactcgctgg atgaaggtga taaccagcaa
    41641 gagcgtttcg ccagctatct cattgccgcc gtgcagcagg caaccaacgg tcactgtgcg
    41701 atatgtgaga cgatggcgca aaaacggcaa tatgccagcc tgacgtcact cttcgcccag
    41761 cttttcattg agctggcgga atggcatagc ccactttatc tggtcatcga tgactatcat
    41821 ctgatcacta atccagtgat ccacgagtca atgcgcttct ttattcgcca tcaaccagaa
    41881 aatctcaccc tggtggtgtt gtcacgcaac cttccgcaac tgggcattgc caatctgcgt
    41941 gttcgtgatc aactgctgga aattggcagt cagcaactgg catttaccca tcaggaagcg
    42001 aagcagtttt ttgattgccg tctgtcatcg ccgattgaag ccgcagaaag cagtcggatt
    42061 tgcgatgacg tttccggttg ggcgacggca ctacagctaa tcgccctctc cgcccggcag
    42121 aatacccact cagcccataa gtcggcacgc cgcctggcgg gaatcaatgc cagccatctt
    42181 tcggattatc tggtcgatga ggttttggat aacgtcgatc tcgcaacgcg ccattttctg
    42241 ttgaaaagcg ccattttgcg ctcaatgaac gatgcactca tcacccgtgt gaccggcgaa
    42301 gaaaacgggc aaatgcgcct cgaagagatt gagcgtcagg ggctgttttt acagcggatg
    42361 gatgataccg gcgagtggtt ctgctatcac ccgctgtttg gcaacttcct gcgccagcgc
    42421 tgccagtggg aactggcggc ggagctgccg gaaatccacc gtgccgccgc agaaagctgg
    42481 atggcccagg gatttcccag cgaagcgatt catcatgcgc tggcggcagg cgatgcgctg
    42541 atgctgcgcg atattctgct taatcacgcc tggagtttgt tcaaccatag cgaactgtcg
    42601 ctgctggaag agtcgcttaa ggccctgccg tgggacagtt tgctggaaaa tccgcagtta
    42661 gtgttattgc aggcgtggct aatgcaaagc caacatcgct atggcgaagt taacaccctg
    42721 ctagcccgtg ctgaacatga aatcaaggac atcagagaag gcaccatgca cgcagaattt
    42781 aacgctctgc gcgcccaggt ggcgattaac gatggtaatc cggatgaagc ggaacggctg
    42841 gcaaaactgg cactggaaga gctgccgccg ggctggttct atagccgcat tgtggcaacc
    42901 tcagtgctgg gtgaagtgct gcactgcaaa ggcgaattga cccgctcact ggcgctaatg
    42961 cagcaaaccg aacagatggc acgccagcac gacgtctggc actacgcctt atggagttta
    43021 atccagcaaa gtgaaattct gtttgcccaa gggttcctgc aaaccgcgtg ggaaacacag
    43081 gaaaaagcgt tccagctgat caacgagcag catctggaac agctgccgat gcatgagttt
    43141 ctggtgcgca tccgagcaca actgttatgg gcctgggcac ggctggatga agccgaagcg
    43201 tcggcgcgta gcgggattga agtcttgtcg tcttatcagc cacagcaaca gcttcagtgc
    43261 ctggcaatgt tgattcaatg ctcgctggcc cgtggtgatt tagataacgc ccgtagccag
    43321 ctgaaccgtc tggaaaacct gctggggaat ggcaaatatc acagcgactg gatctctaac
    43381 gccaacaaag tccgggtgat ttactggcaa atgaccggcg ataaagccgc cgctgccaac
    43441 tggttgcgtc atacggctaa accggagttt gcgaacaacc acttcctgca aggtcaatgg
    43501 cgcaacattg cccgcgcaca aatcttgctg ggcgagtttg aacctgcgga aattgttctc
    43561 gaagaactca atgaaaatgc ccgcagtctg cggttgatga gcgatctcaa ccgtaacctg
    43621 ttgctgctta atcaactgta ctggcaggcc ggacgtaaaa gtgacgccca gcgcgtgttg
    43681 ctggacgcat taaaactggc gaatcgcacc ggatttatca gccattttgt catcgaaggc
    43741 gaagcgatgg cgcaacaact gcgccagctg attcaactca atacgctgcc ggaactggaa
    43801 cagcatcgcg cacagcgtat tctgcgagaa atcaatcaac atcatcggca taaattcgcc
    43861 catttcgatg agaatttcgt tgaacgtctg ctaaatcatc cggaagtgcc agaactgatc
    43921 cgcaccagcc cgttgaccca acgtgaatgg caggtcctgg ggctgattta ctccggttac
    43981 agcaatgaac aaattgcggg cgaactggaa gtggcggcaa ccaccatcaa gacgcatatc
    44041 cgcaatctgt accaaaaact cggcgtcgcc caccgtcagg ctgccgtgca gcacgcgcag
    44101 aaactgctga agatgatggg ttatggggtg tgagtttagc cggataatgc attggatccg
    44161 gtttacatct ttacatcatt caatactcac ccgcgttacg ccatctgttt ctatcaaacc
    44221 aaaccgcacc ggcaagaaac gctccaccac cgcgatattg gtcagcagat ggcatgaagg
    44281 atgggcgacc ttaaattctc ccgcgcccgc cagcgccatc ggcagtacca gttggtcggc
    44341 gagatattcc cccaccgccg ctgggcttgc caggtagcgt ttcacctctt tcactaactg
    44401 cgcggctacc acctcggcac tgacgcgctt ttcaccgacg acgaaaaagc gttcagtgat
    44461 attttcactt tcgacttcca gcgagacggt attacccggc ccctggtcgc gcggcaggtt
    44521 atgaatattc tgttcatgca gggaaaaact ccccgccagt gtagcgattt cacgctcagc
    44581 aacatggcgc ggcacaccag ctaatagaac ttctccacac atctgcacaa tgttcccgcg
    44641 ctcgccaagt tgcaaggtgt taaacgatgc cacgggcgag acttccgttg ccaccacacc
    44701 gcctccggca ggataaaaac cgtgacgtaa cagcgtggtt tgctgatgaa ttcctatttt
    44761 cgccagcagc ggctccagca cccggcggat aaaatccgca ggcggggccg acgggttatc
    44821 ggtgcctccg ctcacttcaa cacgcgaagg tccatcggca aaccacagcg cgggcagcac
    44881 cgtttgcagc accagcgtac agcttccggc actaccgata gcaaagcggt actcgccgcc
    44941 gcgcacggtg ccaggccgga agaccagacg ctgcgacccc agctccgcgc cttccaccgt
    45001 tgccctacaa atttccgcag ccgcttttac cgcggtcaga tgctggcgca acagccccgg
    45061 tttcgctcgc ccggcacgaa tgctggtgat ggtaaatggc tggccggtta tcatcgacag
    45121 gctcagcgcc gagcgcagga tctgcccgcc accttcgccc tgtgcgccat ccagcgcaat
    45181 catccttttc atcatttatc cttttacgca caccacctga cgcagggtat agataacttc
    45241 caccagatcg ctttgtgccg ccatcaccgc atcaatatct ttatacgcca tcgggatttc
    45301 gtcgatcact tcggcatctt tacggcattc cacatgcgcg gtggcacgaa tttgatcttc
    45361 cacgctgaac agttttttcg ctttagtacg gctcattacc cgcccggcac cgtggctgca
    45421 cgaacagaac gactcttcat ttcccagccc acggacgata aagcttttcg ctcccatcga
    45481 ccctggaata attccatatt gaccagcacg cgcagacacc gcgcctttac gcgtcacgta
    45541 gatctcttcg ccaaagtgct gttctttttg cacatagttg tggtgacagt tgatctcttc
    45601 catcgccagc gtttgctgtt gtcttaccgt tttctgcgta acgctctgca acgctgttac
    45661 tacgttttcc atcattgcat cgcggttaag gctggcaaaa agctgcgccc aggccaccgc
    45721 tttcaggtaa tcatcaaagt attccgtacc ttccataaag tacgccagat cacgcgacgg
    45781 caacgtttca agctggtcct gcatctcttt ttgcgccaga tcgataaagt aagtcccgat
    45841 ggcgttacca attccgcgtg aaccggagtg cagcataatc cacacctgat ccgactcatc
    45901 aagacagatt tcaataaaat ggttacccgt tcccagcgtt cccagatgtt tatagttatt
    45961 ggtattcagg aaacgcggat atttttgcgt taaccactga taaccggctt ccagctcagc
    46021 ccatttagca tcgacgttaa caggtggatt ttcccaggca cctttatcac gtttacaacg
    46081 gccagtggta cgcccgtgcg gcacggccgt ttcaatcgcc tgacgcagct ctgccaggtt
    46141 ttcaggcagg tcttccgccg ttaacgcggt acgcagcgcg ttcattccac agccaatatc
    46201 cacgcccacc gccgccggaa taatcgcccc tttggtcggg atcacgctac caatggtgga
    46261 acctttaccc aggtgtacat caggcattac cgcaatatgt ttgaaaataa acggcatctt
    46321 cgccgtatta ataagttgct gacgcgcatc ggcctctacc ggcacgcctt tggtccacat
    46381 ttttaccggg gcattttcag tggtcagtaa ttcgtaattc attttgtttt tctcttttcg
    46441 ttgtttgctg tcctgataat tgcaaccgtc gtgccagaaa attaaataag cagcttaatt
    46501 ttttaattca ttgttttaaa aaagattata tctttacgtc cgtaaccgga gatttcccgc
    46561 aaagccaatt taccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
    46621 cagtggcttt tggctttgtc ggtaccgtac tggattatgc cgggcgcggc agtcagcgct
    46681 ggtcaaaatg gcgtccgaca ctctgtttat gccagcaaga atcgttggtc atcgatcgac
    46741 tggaattgtt gcacgacgcc cgctcgcgct cgctatttga aacgcttaaa cgcgatatcg
    46801 ccagcgtttc gccagaaaca gaagtggtga gcgttgagat tgaactgcat aacccgtggg
    46861 atttcgaaga ggtctacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
    46921 aaaaagaaga ctatttaatt cacatcacca ccggtaccca cgtcgcgcag atttgctggt
    46981 ttctgctggc agaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
    47041 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
    47101 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
    47161 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
    47221 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
    47281 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
    47341 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
    47401 aaggcgcgtt taccggggcg cgggaatctc gtgaaggttt attacgcagc gccaacggcg
    47461 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaagca atgctgctaa
    47521 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
    47581 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
    47641 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
    47701 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
    47761 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
    47821 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
    47881 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
    47941 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
    48001 aaaacatcga cctttttgac agactgcaac tggaacacgt gatagctctc tgtcgccagg
    48061 caaagtcgct ttccgctgcc ggacgcctgc tttttgatgt ttcgcgccag ggcaaagcca
    48121 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
    48181 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
    48241 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
    48301 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
    48361 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
    48421 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
    48481 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
    48541 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
    48601 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
    48661 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
    48721 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
    48781 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
    48841 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
    48901 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
    48961 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
    49021 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
    49081 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
    49141 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
    49201 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
    49261 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
    49321 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
    49381 aagtaacgcc agaactcaaa tttcagtgtt ggatcgaatg gccaggccag ccataacatc
    49441 acttcctgat cgccgagaat ttgcatggcg ataaacacca ccacgcaggc gatcatcacc
    49501 acccaggtta ccggacctgc gcgttcacgc aaggcagcaa agaaaggata acggcgataa
    49561 tgcagaccac tgccggtatg gcccgcctgc cagctggccg ccagataacg cggatctgcc
    49621 gggttttcga gaaaacgcgc cagctccgcc cgcacgcgct cggcctggga ctcatccgcc
    49681 agccagacat cgctttggtt atgttgttga atcgtaagga taacaccctg cgtcgccatg
    49741 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
    49801 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
    49861 ctgcgggaaa ctgacgctgc caggcttcaa aaccgccgtc aatgctatag accacatcgt
    49921 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
    49981 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
    50041 aatggaaagc ctgcaccgca tgccccattg cgaaactctg tggatcgcga atatcgacca
    50101 gcaccgcctc tttttcctgc aacttctggt gcgcgacggc aacgttaata cattcgaact
    50161 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgaac agtttacgtc
    50221 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
    50281 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
    50341 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg atagggggcg gcatcaatgg
    50401 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
    50461 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
    50521 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
    50581 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
    50641 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
    50701 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
    50761 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
    50821 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
    50881 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
    50941 tagctggcaa gcgcgcggcc tggttaacgc caccggcccg tgggtgaaac agttcttcga
    51001 cgaggggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
    51061 ggtgccgcgt gtgcataccc agaagcaggc ctacattctg caaaacgaag ataaacgtat
    51121 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
    51181 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
    51241 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
    51301 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
    51361 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
    51421 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
    51481 cattggcccg gcgtggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
    51541 cgacgattat gccgctcgcc tgcgccgccg ctatccgttc ctgactgaat cgctggcgcg
    51601 tcattacgct cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggaacggt
    51661 aagcgatctc ggggaagatt tcggtcatga gttctacgaa gcggagctga aatacctggt
    51721 ggatcacgaa tgggtccgcc gcgccgacga cgccctgtgg cgtcgcacaa aacaaggcat
    51781 gtggctaaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggagt atacgcagca
    51841 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgtg aagtgcaacc gggctatttg
    51901 gattccttag actattcatt gtaaataatt gaataacggc ccctgagcta aaatcgccaa
    51961 acttaagcac gttacccaat cgccccactt cttcctctgg tttctatttt ttctgcacca
    52021 atggcaataa tatcccaatg caaaaccaag ctgtgcacta atcgggccga caaaatcagt
    52081 ccaaggctgc ggccaaaaag gggaaaaatt caggggaagc cagacaaacg tcagaaagaa
    52141 atttcggata ttttccagca acgctgattt ctctggagaa atataaagaa tgaaattcga
    52201 gagaattaac cagaaataga ccggcgaaaa gaccagccag caatgcagtc tgttttttaa
    52261 ttcaggaggc gttctgcggg agaattgcca accgatgcca gccattacac tgaaaaaaag
    52321 tgcatattca accattaact caagttgtag ttcctgaaga gaaatacgca caaccaggag
    52381 cggaaaggag aatattattt ctgccagaaa agttaacgtg aatataagta cggctaagac
    52441 aataaccgtc ggccatacca ataatggcac gataacaaaa ccgagaatta cagggggaat
    52501 ataaagccgg aatttaatca tttttatttt cctggtgatt cggatgatgc gtcatacatc
    52561 gcgcataaac actcatcaat tatccagtcg tctacccaga cctgatgctg tgtgccctgt
    52621 tctggctgct ttggtgggtt ctcaatcgct tgccgcgctc tttctttcag taaaggatga
    52681 ttatttctag cccaggcatt gatttgggtg gtaatggcgt tatcgatatc gtcctggtta
    52741 tctctgatat aatcctcaca atatctgttc aataaacagg caagagaaac ggtcgaccaa
    52801 aagccgggaa ctgaattaat ggcatccagc aatggcgcaa gagcgatatc ggcacggaaa
    52861 aacatttcca gcatatactg taattcgaca gcttcatcgc cggataccca atcagtcata
    52921 tactggcagg caaatcgctg taaaattgcc cgttcatccg cacgccagtt ttctttgttg
    52981 gcaaggttga tacggcgcag agaaaaaact acgtgaatat tgtcaaaatg cccctgtgca
    53041 acaaattcca gtaaacgcgg cagaaaatag cggacctcat cgctattttg cggatcaggc
    53101 cctgggctac tattccaggc attgattaag gaatacggga tcgctcgtaa cgatgtactc
    53161 cgtaatgcct tttgttcctg ctgactcagg caatattcac aacaaaccac aaatttctgc
    53221 ggcacccgat aacgacgaaa ggtgtgataa atctgctcaa ttaatgcctg catggcgggg
    53281 ctgggttgca tagttacccc ctcgcccgct gatcgcgtgt aaacggttga tagacaaaac
    53341 gagcattcgc cgcgttcagg cgaataaggg cgctacgcga aagggaaaag ctggggcgaa
    53401 tgcaactatt gcagtctcga cagctttcct ctgcgcaatt ttccactgag taatgacgcg
    53461 tgtagagttt cagttcggcc tgcttatcgc gaaatccgcg taaactgtta ttgttatgaa
    53521 atttccaggc atctttaaag acctgaaaga tatagacatc ctcaatccag gagtcacatt
    53581 ttttaagcgg gcgctttgct attatcaccc gctcatccgg atttagcatc gccgtaaatt
    53641 gtacggtgac gtcatttgtc gtgttcaggt cacattgaac atctggtaca tcactgatat
    53701 aggggtcaaa tccaaaatca gcaccacagg cacaaagacg gctaatggtg gcggtatcga
    53761 gggaaaatcc cggccatacc ttgctggagg ttaagccaat atttaatgtg acatcatatc
    53821 gaagcaatcc ctcaagaccg tcagcaccga cgaacttttc cccttcatcc agggcctggt
    53881 taataagttg ctgaacaatg ccttcatccc agtatgccga tttgcagttg gcaatcgtaa
    53941 tatccacgcc tgtgaaggga tcagtaccgg caaggcttgc aaataatgta ttaactaatt
    54001 ccatttttat tatccagctt taaggtagcg tcttatacat cgggacataa cctacatctt
    54061 ccaccaaact ctgtccctgc ggcgttaaaa accactccaa caatttttgt gtctctgacg
    54121 tcgtattttc ccgcgttacc ataaaggcat cgacgatata gggatatttg ccgttgcgaa
    54181 tgttttcggc agtcggtgca ataccgttaa tcgctagcag ttttatattt ttatcgtcgt
    54241 tcatttgtgt tgcgtaataa cggaaggtgt aacctattgc gttgtttgta ttacggtatt
    54301 cggcaacgac tttaatcatt ccctccatca tacttgccac ttctgtttcc tgcggcgaga
    54361 tcatgcggac atttttcatg acctgggatt gcatcaccgt ctggctacca gagtcttccg
    54421 ggcgttgcca ggtctggatc tcctgatcgt taccgccaac cgtgcgccag ttggtaattg
    54481 caccactgaa gatgtcacgc acttgttgtt cggtcaggga attaaccggg ttatctgcat
    54541 tgacgatgaa aacaaacgct tcacgggcaa aaggcgtgta aatcaaagtg acgcccgatt
    54601 cctccgcgcg ttttttctgc ccaccggaag gttgagccac aaagataata tcggcattac
    54661 ctttaacaat cttgttatat gcttcgggag tacgagagtt cgccagatat tcccattcgt
    54721 gaaaatcttc cggcaaaacg cttagtgcat aaaaggcaga ggcataaatg gggtacgccg
    54781 ccgttgcgcc atcaagtcgc ggccagtttt gcgtgaactg aatttgcggt ttaccacgca
    54841 gtggtgtcag ttgattattg agtttatcgg gtcgccaggc ccaggtatca atattttcac
    54901 gcacaccgac gccgggatta agctgtttat ataaataagc ctggtaaccg gcgacaaaac
    54961 caagcatgag aaggataaac aagagagaat tacggcaacg taacccggac gtgcccgtta
    55021 aaggatatct ggtcggccag ccgtacccca gcgaaaaact gatatgcgcg ccaacaggta
    55081 taatgactaa ccaccagtac tcaccaccga ctaatgaggc taacaaaacg cccagccagg
    55141 ggagcataag atcagccaac caacccgctc ccatctggtg aaattgataa tctgccagat
    55201 gcagcgcgat tatccagacc cccagggtta ataatgcggg tactaaaaaa agacattgtc
    55261 gccagacggc tatttctctt ttttctggtg cactatcgtc cgcccacact cgccccgaga
    55321 aaaatagcat taatgcgccc agcgccagca taaaaatgtt gagcagaagg taaattgttg
    55381 gcgtgataaa gatattacaa acttcaataa taccggcaag tccgacaccc agcatggcaa
    55441 taaccgccgc caaaaactgc gccagaatgg ggatgccgaa aaaagtcatt accagcgagg
    55501 tcaaaatcca ttttctgttt tgcattattc tttccattct ttctgaatgg cgaaattata
    55561 ctccccgagt ccccttgccc cttctggaca cttttccgaa atgatggcga aaaaaacggg
    55621 accctttggc cccgttctat ttattagtga aattacaatc tcaccggatc aatatgccag
    55681 atatgatcgg cgtactcttt gatagtacgg tcagaagaga agtagcccat attggcaatg
    55741 ttcagcatcg cttttgcggt ccactcttcc tgaagctcgt agagttcgtc gactttatcc
    55801 tgacaatcga catagctgcg ataatccgcc agaacctgat agtgatcgcc gaagttgatc
    55861 agcgaatcaa ccagatcgcg atagcgaccc ggatcttccg gactgaatac accgctgccg
    55921 atttgcgtca gcacctgatg cagctcctca tctttctcgt agtattcacg cggtttgtag
    55981 ccctgacgac gcagttcttc cacttcttcc gctgtgttac caaagataaa gatattgtca
    56041 gcaccgacat gatccagcat ctcgacattc gcaccgtcca acgtaccgat agtcagcgca
    56101 ccgttaagcg caaacttcat gttactggtg ccggaagctt ccgtccccgc cagcgaaatc
    56161 tgttcagaca gatcggccgc cggaatgatc aactgcgcca ggctaacgct gtagttcggg
    56221 atgaacacga ctttcagctt gtcgccaatc tgcggatcgt tgttgatcac tttcgctacg
    56281 tcattgatca aatgaataat gtgcttcgcc atgtaatagg ccgaagccgc cttaccgcca
    56341 aaaatattca cgcgcggtac ccacttcgca tccgggtcgg ccttgatgcg gttatagcgg
    56401 gtaatcacat gcaacacatt catcaattga cgtttgtatt cgtgaatacg tttgatttgt
    56461 acatcgaaca acgcctttgg attcaccacc acattcagct gctgggcgat atactctgcc
    56521 agacgctttt tgttctccag cttcgcctga tgcacagcgt gattaaccat tgggaaatca
    56581 cagtgttgtt gcagctcatt aagcaggcta aggtcggtgc gccagttacg gcccaggtgt
    56641 tcgtccagca cggctgaaag cgatgggttc gctaccgcta gccagcgacg cggcgtcaca
    56701 ccgttggtga cgttggtgaa acgacccggg aagattttcg caaagtcggc aaacaacgat
    56761 tgcaccatca gattagagtg cagttccgat acaccgttaa ctttgtggct cacaacaacc
    56821 gccagccagg ccatacgcac acgacgaccg ttggattcat caatgatcga cgcccgtccc
    56881 agcagatcgg tatcgttcgg atactgttcc tgcaaggttt tcaggaaata gtcgttgatt
    56941 tcaaagatga tctgcaggtg acgcggcaga attttaccca gcatatcaac cggccaggtt
    57001 tccagcgcct cgctcatcag cgtgtggtta gtgtaggaga agacctgaca acacacctca
    57061 aacgcgtcgt cccagctaaa ttggtgctca tcgatcagca gacgcatcat ctcaggaatc
    57121 gacagtaccg gatgggtatc attgagatga atcgcgattt tatccgccag gttatcgtag
    57181 gttttatgca actgataatg gcggcttaaa atgtcctgaa tggtcgagga aaccaggaag
    57241 tattcctgac gcaggcgcag ctcacgcccg gagtaggtgg agtcatccgg atacagtacg
    57301 cgagatacgt tctcggagtg gtttttatct tccactgccg cgaagtagtc accctggttg
    57361 aatttaccga ggttaatttc gctactggct tgcgcactcc acaaacgcaa cgtgttggtc
    57421 gcgtcggtat cgtaaccagg gattatctga tcgtaagcga ctcccagaat ctcttcggtt
    57481 tcaatccagc gcgttttttt accttcctgc tgaatgcgac caccaaaacg gactttatag
    57541 cgcgtgttgt ggcgtttgaa ttcccacggg ttaccgtatt ccagccagta gtctggcgac
    57601 tctttctggc taccgttaac gatgttctgc ttgaacatac cgtagtcata gcggatgccg
    57661 taaccgcgcc ccggcaaccc taacgtcgcc agagaatcaa ggaagcaagc cgccagacgt
    57721 cccaggccac cgttaccgag gcccggatca ttttcttcat caatcagctc ttcgagattt
    57781 aaccccatcg cttccagtgc gccctgtaca tcttcgtaaa ttcctagcga caacatggcg
    57841 ttggagagcg tacggccaat caaaaactcc atcgacaggt agtaaacctg acgagtttct
    57901 tgcgacaact gggcacggtt tgaacgtaac cagcgctcca cgagacgatc gcgcacagca
    57961 aataacgttg cgttcagcca ttcatgttta ttggcgacga ccgggtcctt tccaatcgta
    58021 aacatcagct tgtaagcgat agagtgctta agagcttcta cgctaagcgt gggcgatgaa
    58081 tatgtaaacg gagcattcat ataggcgttt cctgaaaact atttcaagcg atagtaaagc
    58141 tcacggtacg acttcgccgc gacctgccag ctaaaatcca ttgccatagc ctgacgttgc
    58201 acaaaccgcc acagtgaagg acgggaccac agtacaaaag cacgccgaat agcccgtaac
    58261 agcgaccagg cattactatc ttcaaagaca aacccactgg cgacgccatc tgcaaggttc
    58321 tcaagagaac agtcagaaac cgtatcagca agcccaccgg tgcgccgcac taacggcagc
    58381 gtaccgtact tcaatccata aagttgcgtt aagccgcacg gttcaaaacg gctgggcacc
    58441 agaatgacgt ccgcgccgcc cataatgcga tgcgaaaatg cttcgtgata gccaatctga
    58501 acgcccacct gaccggggta ttccgctgcc gccgcaagga aaccttcctg cagcaccgga
    58561 tcgcccgcgc cgagtagcgc cagctgcccg ccctgctcca gaagacccgg taaggcttcc
    58621 agcaccaggt cgagaccttt ctggctggtc agacggctca ccactgcaaa aagcggcact
    58681 ttatcgtcaa ccttaagccc cattgcgatt tgtaactggc gcttattttc cgctttatct
    58741 tccaacgtat cgcgggtgta acgcgaggcc aacagtaagt ccgtctctgg actccagatt
    58801 ttctcgtcca cgccgttcag tacgccggaa agacgccctt cacggtgacg ctgttgcaac
    58861 agaccttcca taccgtaggc aaactgcggt tcggtgatct cgcgagcgta ggttggactg
    58921 accgccgtaa tgtgatcggc atagtacaga ccggccttca ggaaagagat ttgtccgttg
    58981 aattccagcc catgaatatt aaagaatgac catggcaatt ggatgtcatt catatgatgt
    59041 gcataaaaca tgccttgata ggccaggttg tgcacagtaa acaccgactt cgccggacgc
    59101 ccgcgcgccg ccagatacgc aggcgcaagg cctgcatgcc agtcgtgcgc atgcaccaca
    59161 tcaggacgcc agaatgggtc aagcccgctg gccatttctg ccccaaccca ccccagcagc
    59221 gcaaaacgca atacgttgtc ggtataggca aataagttgg tatcgtgata cgggcttccc
    59281 ggacgatcat agagatgcgg cgcgtcaatc aggtatatgc caaccccgtt gtaatgaccg
    59341 aacaacagcg tgatatgtcc ggcgaaggta tcacgacggg atactacctg cgcatcggta
    59401 acgccacggc gaatatcggg aaatgcaggc aacagtacgc gagcgtcaac gccgtctgcg
    59461 atttgtgctg cgggtaatgc cccaataaca tcagccagac cgccggtttt aagcagcggg
    59521 aacatctctg aacatacatg taaaacctgc attatcgctc ctgtttatgc cctaacttcc
    59581 gtagcatttc gcgcgttacc agcacgatgc cttcttctga acgatagaaa cgacgtgcat
    59641 cttcctctgc gttttcacca atcaccatgc cttccggaat aacacaagca cgatcgatga
    59701 cgcagcggcg cagacggcac gagcgaccta cccatacttc cggtaacaat acggcggaat
    59761 caatgttgca gaatgaattc acgcgaacgc gcgagaacag aacggactgc accaccaccg
    59821 aaccggagat cacacagccg ccggatacca gtgagttaag agtcatcccg tggctaccgg
    59881 agcgatcctg cacgaatttc gctggcggta atgattcatt gtaggtgcga attggccaat
    59941 tgcgatcgta catatccagt tccggcacca cagaggccag atcgaggttc gctttccagt
    60001 aagcttccag cgtacccaca tcgcgccagt acggctcggc atccgggtcg gattgtacgc
    60061 aagagagcgg gaacgggtgc gcataggcca gaccggcttc ggtgatcttg ggaatcaaat
    60121 ctttgccaaa gtcgtggctg gagttctcat cacgatcgtc ttcttccagc agttcataca
    60181 gatagtcggc gtcaaagacg tagataccca tactcgccag agatttgctc ggatcgttcg
    60241 gcattgacgg tgggttagca ggtttttcaa cgaattcgat aattttatcg ttctcatcaa
    60301 ccgccataac gccaaatgcg gaggcttctt caatcggtac tggcatacaa gcaacggtgc
    60361 aacgtgcgcc tttttcgacg tgatcgataa gcatacgcga gtagtcttgc ttgtagatat
    60421 ggtcgcccgc caggatcacc acgtattccg ctttataacg gcggataatg tcgaggtttt
    60481 gggtgaccgc atctgcggtg ccgcgatacc agttttcccc tttcattctc tgctgtgctg
    60541 gcagcagatc gacaaactcg ttcatttctt cattgaagaa tgaccagccg cgctgaatgt
    60601 gctgcaccag agtgtgggac tggtactggg tgatcacgcc catacgacgg atcccggagt
    60661 tgatgcagtt agacagcgca aagtcgataa tgcggaactt accgccgaag tgtacggccg
    60721 gttttgctcg cttattggtt aaatccttca ggcgggtacc acgtcctccc gccagtatca
    60781 gggcaacaga tttcaatggc agctggcgcg ccaacattaa gtgatcgttc ttctctaaac
    60841 taaccatgac taactccttt tttatcatct ctggaacaca cacaatccgt gtgcaggtcc
    60901 ctgccagaca gccgtaatca ctgggttatc ctctccagcg aatgggggaa tggcgtgcca
    60961 ctccccagca ggtaaaacaa tctctgttac ctcaagcgtg gcgttaattg cgatcaaaaa
    61021 gcgatccgag agcagaattt gcagctgttt cggcccgttt tgccactcat ccgtgcttaa
    61081 aggttgagca tatcgattta gccaacggac attgccgtcg ccttcttccc accagcgatt
    61141 ctccaccaaa gcgggaatgc gcttgcgcag atggattaac gcggcggtaa atgcggttaa
    61201 accactgctt gcctgcgacc agtccaacca ggttaattgg ttatcctgac agtaggcatt
    61261 gttattgcca tgctggctgt gaccatgttc gtcaccggcc agtaacatcg gcgtaccctg
    61321 ggagagcaac aacgttgtta acagggcgtg aatgctgtcg cgccgccgtt caaccaggtc
    61381 aagagaaccg cctaaccctt ctttaccatg attgttactg taattgttgt tggtcccgtc
    61441 gcgattttct tctccgtttg cttcattgtg tttatggttg aagcaaacgc agtcgcgaag
    61501 cgtaaaaccg tcatgcgcgg tgacgagatt aatcgcggca ctcggcagac gaccattacg
    61561 tttaaaaaca tcgctggagg cagcaaaacg cccggcaaac gcccccagag gcaaatcata
    61621 atgtagccag aaacgacggg cagcatcgcg gaaatgatcg ttccactcgg caaacagcgg
    61681 cgggaaattt cccacctgat aaccaccagg agcgatatcc cacggttcag caattaactt
    61741 cacctgcgag agcaccgggc agttctggat agcggtaaac aacggcgcat cctgacggaa
    61801 ctctggcgta cggcccatga ctgccgccag atcaaagcgg aaaccatcga cgtggcaggt
    61861 ttctacccaa taacgcaggc aggcgctggc ataatccacc accgccggat gactcaaatt
    61921 gagcgtgttg ccgcaaccgg tccagttgtg ataatcgccg tcttctctta tccaataata
    61981 gctacggtta tcgatcccac gcagcgagaa taacgggccg tcgaggtcca gttccgcact
    62041 atggttgagc acgatatcaa gaatgacttc gatacccgct ttatgcagtg ctttgattgc
    62101 atcgcgaaac tcatccagcg ccgtttctgg cgagcaggca tacgccggat gcagcgcaaa
    62161 catcgccacc gggttgtaac cccagtagtt acttagcgcc atgcgttgca gacgtggttc
    62221 actggcaaac tgcgccactg gcagcagttc cagcgcggta atgcccaatt gtttcaaata
    62281 gttgatcatc accggatgcc cgagggcttt ataagtgcca cggatctcga ccgggatctc
    62341 cgggtgcaag tacgttaatc ctttgacatg ggcttcataa atgatggtgc tgccccacgg
    62401 cgtgcgcggc ggggcatcat cttcccagtc atagtgatca accaccacta cgcatttcgg
    62461 cgcaatggcg gcgttgtcgc gatagtcagg ttcattatga ccggcgtgca gcagcgggtt
    62521 atctttaaac tccccctcaa tttgccgcgc gcaaggatca atcaacaact tcgccgggtt
    62581 aaagcgatgc ccctcggcgg gttgccaggg gccatgaacg cgataaccat aacgcaaacc
    62641 cgggcgcgca tccggcagat aaccgtgcca aatgtcgcca ctgtgccctg gcaagtcata
    62701 gcgatgttcc tggccattgg cgtcaaagac acacagttct acccgctcgg catgagcgga
    62761 gaaaagtgtg aagttgacgc cctgaccgtc gtaatgcgcg ccgaggggag cgggtttgcc
    62821 aatggcgagt tgtgtcattc tgcctcccga accagccaga tagtggccag cggtggtagc
    62881 gtcaggctta gtgaatgctg acgaccgtgg ctggcaatct catcgctgtg taccgtgccg
    62941 ccattgcctg cattactgcc gtgatagtgc atggaatcgg tattgaggat ttcacgccat
    63001 ttgcccggct ggtttatgcc gaagcgataa tcatgacgcg gcaccggcgt aaagttactg
    63061 gcaacgatga tttcgttacc ctctttatcg cgacgcacaa agatcagcac cgagcgttct
    63121 ttgtcatcca ccaccagcca ttcaaagccg tacgggtcaa aatccagttc atgcattgct
    63181 ttatggtggc ggtaggtgag gttcagatcg cgcaccagac gctggacacc gtggtgccag
    63241 ttatcgccgc cttccaacag atgccagtcg aggctggcgt catggttcca ctcgcggccc
    63301 tgggcaaatt cgttacccat gaacagtagt ttcttgcccg ggaatgccca catccagcca
    63361 tagtaggcgc gcaggttcgc gaatttctgc catgcgtcgc ccggcatgcg gtcgagaatc
    63421 gattttttac cgtggaccac ttcatcatgc gacaacggca ggacgaagtt ttcagtgtag
    63481 ttgtagagaa tcccgaaggt cagtttatcg tgatgatact gacgataaac cgggtcgagc
    63541 ttcatgtagt ccagggtgtc atgcatccag ccgaggttcc acttgtacca gaagcccaga
    63601 ccgcccatat cctgcggacg agaaacgcca gggaaatcgg tagactcctc agccattgtc
    63661 accgcaccgg aaacctgctc accaagaata cggttggtat tacgcaagaa ttcaatcgct
    63721 tcaagattct cgcgcccgcc aaattcgttc gggatccact ccccctcttt acggctgtag
    63781 tcgcgataaa tcattgacgc caccgcatcg acgcgcagcg catcaatacc aaaacgttca
    63841 atccagtaaa gcgcgttacc gacgaggaag ttactgactt cacggcgacc atagttgtag
    63901 atcagcgtgt tccagtcctg atgatagcct tcacgcggat cgctgtgttc atacaagttc
    63961 gtgccatcaa attcggcaag cgcaaagtca tcagtcggga agtggcctgg cacccagtcg
    64021 agaatcacgt tcagaccagc tgcgtgtgcg gcatcaatga aataacggaa gtcgtcgcga
    64081 gtaccaaaac ggcgggttgg tgcatacagg ccggttggct gataacccca actgccatcg
    64141 aagggatgct cgttaatggg cagtagttcg aggtgggtaa agcccatcca tttagcataa
    64201 ggcaccagtt gatcggccag ctcgcggtag ctcaaccaga aattgttgtc ggtgtggcga
    64261 cgccaggaac ccaggtgaac ttcataaata gagattggcg catcaaactg attcgctttt
    64321 ttgcgctctt cagtctgtac aaccttttcc ggcagcccgc aaataagaga cgcggtttcc
    64381 gggcgcattt gcgcttcgaa ggcataaggg tcggacttca gacgcaagtt gccattggca
    64441 tcaatcatct cgtatttata gagctgaccg ttatgcgccc cagggataaa cagttcccag
    64501 atgccgctct ctttacgcag gcgcatcggg tgacggcgac cgtcccagta gttgaattgc
    64561 ccaaccaccg agacccgacg ggcgtttgga gcccagacag agaaacgcgt acctgtgacg
    64621 ccatccatag tatctgcatg cgcgcctaag gtttcatacg ggcgcaggtg agtaccttca
    64681 gataatagcc aggcatccat ttcctggatt agcggaccaa aacggtaagg atcatcaatc
    64741 aggttttgct gaccatgcca gacaacagcc aactgatagc ggaaaaaatt cttacgtcgc
    64801 ggaatgacgc cgctaaagaa tccccgtgag tcgagacact ccagttttgc gagtttgcgc
    64861 ccggttttcg gttcaatcac ccacacatcg gtagcgtcgg gtaaaagggc acggacttcc
    64921 agtcccgcgg tggttttatg cattcccagt acggaaaaag gatccgcaaa atggcctgca
    64981 attagcgcgt taatcacgtc tctatcgata cgatcggaca tgcttgtctt cctgttttat
    65041 tgtgtcaccc catccaattg gatttttgac ttctggttgt gacatttttt tgacctgaac
    65101 ggcgcagcac tctgtgcatc ctctctgcgt cgtcctcact tcaggtaagg ctgtgaatac
    65161 tcatgtattc agccaccctt aaagaatagc caatgctcta tttaactccc ggtaaatcat
    65221 gaaacatctg cgcttactcc tgtattacgc actaacaggg gcggcatcgc gccccagatt
    65281 taatgaataa agattacgcc agttgacgaa gcatccgacg cagcggctcc gcggcccccc
    65341 acagcagctg gtcgcccacg gtaaaggctg acaggaactc tggtcccata ttcagcttac
    65401 gcaggcggcc taccggcgtg gtcagcgtgc cggtaacggc agctggggtt agctcacgca
    65461 tagtgatttc ccgatcgttc ggaacgactt tcgcccacgg attgtgcgca gccagcagtt
    65521 cttccacggt cggaatagac acatcttttt tcaatttaat agtgaatgcc tggctgtggc
    65581 agcgcaatgc cccgacacgc acacataaac catctaccgg aattacggaa gatgtgttga
    65641 ggatcttgtt ggtttccgcc tgccctttcc actcttcgcg gctctgaccg ttatcgagct
    65701 gtttgtcgat ccacggaatc aggctacccg ccagcggcac gccaaagtta tccaccggca
    65761 gctcaccgct acgggttaag gttgtgactt tgcgttcgat atcgagaata gcagaggacg
    65821 gggtcgcgag ttcatctgcc acatggccat acagatggcc catctgggtt aataactcac
    65881 gcatatgtcg cgcaccaccg ccggaagcgg cctggtaggt tgcaacggac acccaatcaa
    65941 caagatcatt ggcgaataaa ccacccaacg acatcaacat caggcttacg gtacagttac
    66001 cgccaacaaa agtcctgatg ccattattta atccgtcggt aatgacgtcc tgattgacgg
    66061 ggtcaagaat gatgatggcg tcatctttca tgcgcagaga cgatgctgcg tcaatccagt
    66121 aaccttgcca tccgctttca cgaagctttg gatagatttc gttggtataa tcgccgccct
    66181 gacaggtcac aatgatatcg agggccttta gcgcctccag atcaaaggca tcctgaagtg
    66241 tgccagtggt tccgccaaaa gacggcgcag cctggccaag ctgagaagta gaaaagaaga
    66301 cagggcgaat ggcgtcgaag tcgcgctctt caaccatgcg ttgcatgaga acggagccga
    66361 ccataccgcg ccagccgata aaaccaacat ttttcataag cgtttttttc ctgcaaagat
    66421 gtgtgctgta taaatgtgcc ggtctcctct tggcacatct ttcaccatac aaaaagcagc
    66481 caaagtcgca agtgaaatta atcaatgata gcgaagccat cagtaatgcg acttatcctg
    66541 ctttgttagc acgcagaaag tccgcggcaa ttatcaggga atttgagtta tgaatgaaat
    66601 catttctgca gcagttttat tgatcctgat tatggatccg ctcggaaacc tacctatttt
    66661 catgtccgta ctgaaacata ctgaaccgaa aagacggcgg gcaatcatgg tgcgagagtt
    66721 gcttattgct ctcctggtga tgctggtgtt cctgtttgcg ggtgagaaaa ttctggcatt
    66781 tctcagcctg cgagcagaaa ccgtctccat ttctggcggc atcattctgt ttctaattgc
    66841 cattaagatg attttcccca gcgcttcagg aaatagcagc gggcttccgg caggtgaaga
    66901 gccatttatc gtgccgttgg caattccgtt agtcgccggg ccgactattc tcgccacgct
    66961 gatgttgttg tctcatcagt acccgaatca gatggggcat ctggtgattg ctctgctgct
    67021 ggcctggggc ggcacctttg tcatcctgct acagtcttcg ctatttttac gtctgctggg
    67081 cgagaaaggg gtgaacgcac ttgaacgcct gatgggattg attctggtga tgatggcaac
    67141 ccagatgttc ctcgacggca ttcgaatgtg gatgaagggg taaggttcaa acaggtgctg
    67201 cacacaacca gacgcggcac ctgttttatt acctgaactg ataatgacct tcgcccaatc
    67261 ggataaccct tccctcttct tctgcgcggt tcagccacac actaatttgt tcgatggtta
    67321 aaccagtaca agacgacagc ctttcaatac ttatcgattc ctttgcaaag atcgccagtt
    67381 ccgccacaaa gagttgataa aaatcgacag gcgtatgcgc gggcattact gcgtaatttg
    67441 cgtcatcttc atgcagcgaa aacagttctg gttgagcgtt acggagtgct ctgtcataca
    67501 tcagcccagg aacgtctgac agtctgatcg gcacattctc agcctgttca gcactccatg
    67561 acgccccatt ttctactaat cgtgaattgg ctgattgcat atcctgattt ggtttaacct
    67621 gcacaggcag ccactgatgt tttaatgcct ctatcgcccc ggttatcgtt ccaccggtca
    67681 ttcccgcacg tacaaccatt gcgctttcag caaggcaata aatatattta tttcgcgcca
    67741 tcgcattgcc gacggtgaaa cgcacctctg ggtaaaacgg cgaaatcaac accaggttgc
    67801 ctgctataag cccttcacgc catttcacta acgtactcgt ttttaacaag ctatcagcta
    67861 atacgccaac ggcagttccc ccggcctcca gtgccgacgc cattgcacat tcatcaatac
    67921 ctcgcgcacc accagagata acgcaaatcc cctgttgggc cagtttcgcg gccagttgtt
    67981 gggtatagcg caaatcgtca gtcggagcat ctcgcgagcc aacaatcgcc ataccttccg
    68041 cttgcagtaa tgctttattc ccgcagccaa ataaaacggg aggtgcatcc gttcgcaatc
    68101 ggtttttcaa gcgaacggga taatcagcat ctcctcgggt tatgatccat aaaccggcgc
    68161 gttgccactt atctaccgcc aacgccagac tatgaccgcg cgccagcaag ccaagaatac
    68221 gctcctgaga aatacgcgga tcgctccagc ctgtaagttt ctcttgcggt tgcggcacca
    68281 gaagctcggc gggactgata cgttggtgct tcagccataa tgcaaacttc ccccattcac
    68341 tattactaag aggtttatgc tcactcgccg ccgcgcgaga aaaatcgctg gttagcagga
    68401 gagtcgcttg tgcattggct gaaagattca tcaatttttt accgaggtag aagcaagggc
    68461 aagaggataa accgtcgggc aacctgcctg gcgtaacagt gctgtcagaa ctgtcagcgt
    68521 ccatgcagaa tccacgatat cgtcaaccag caacgccgga cctggcatca aaggaggggt
    68581 aatcacaaac gccccgtcga gattttgaca ctggtgaaaa cggttttgct gcattttctg
    68641 cggtggattg tccacgactt tttcaatggc atcaataaaa ggtaagccaa gtttcgccgc
    68701 cagtcgccgg gcaaaatcag gaaccaggtc gaggtgcctt aatgaaggaa cgcagcatac
    68761 ccaggttggt tcaggatgcg gattccagcg ttggcgaacc atttccgcac atgcctccac
    68821 cagctcatca ctgaatcgcc ccgttttttt ctcctgtgct acctgctttc cccatccgga
    68881 atctccccaa cgagaaagga ctcgccccgt agatccttgt aaacccgcag gcaagttccc
    68941 tttaaatccg tattgcgtaa aagctccaga tgctacctgt ttattgagat ttaatggcag
    69001 gtcagcgtgt ttaataaata aatttgccgc atggagtaag ccgctatcaa tatcgggcga
    69061 caacaacgga tgctgaagac aactgctaca tttaccacaa cgttcggcac ttggctcatc
    69121 gagtgcgcgg cgcagaaatt gcattttgca ctccttggtc gtgatatagc tttcaacatc
    69181 tgccagctca ttttttctct gattcatcaa atggttaatt cgttcatggt caggagagaa
    69241 actgacgata gttctgcgcc ataatttctc ggtatacacc acaggcgatg gattttcagc
    69301 taccaataat ttcagtgctt tttctatttg cccataacgg agattcgtcc gttgttcaat
    69361 gcctcgtagg gtaagaccgt cattctcgct aagtacgttg agtatttcat gaatttgcgc
    69421 ctccgcagga aaggcgcttt cacggaagaa tttatgaata gcgcggtctt caccaccaca
    69481 aagcaatatg ccaacagcgg aatctatagc acgcccggca cgccctacct gttggtagta
    69541 accgacaata gaccccggca tctggtaatg aataacaaac cccaaatccg gtttgtcaaa
    69601 gcccattccc agcgccgtcg tcgcgaccag aactttaatt ttatttgcca gcaacgcctg
    69661 ctccagatat tccctggcag tattagaatc ttccgcgcct tcacaagtca cgccgctgta
    69721 gtaagcaaat gcactgatac catttgtcct taaccactcg gcgacaaggt cggcatcgcg
    69781 agtagtcagg gtataaacaa ttcccgattt ggaaaactga gggataaccg ttgctaacca
    69841 tgccagacgg gatgactgtt cacctaagac taaggcatca agcgccaaac tttcgcgagc
    69901 cagcgttcca cgctgaatca caatgtcacc caattgctga cggatatcct caacgacacg
    69961 gttattcgct gtcgcggttg tacccagaac tggggtattc gcaggcagtt ggcgcaaaat
    70021 atctaatata cgtcggtaat ccgggcgaaa atcgtggccc caatcagaga tgcagtgcgc
    70081 ctcgtcgacc acaagcaagc caatacgatc ggctatagga tataaaaccg tttcgataaa
    70141 gtcttggttt gccaaacgtt ccggggaaac cagcaagcaa tcgacctcac cttgcagcaa
    70201 cttatcgcta atacgctgcc attcttccct gtttgtagaa ttcaacgttt cagccgtaat
    70261 acctaaccgt tcagcggcgg caacctggtt acgcatcagg gcaagcaaag gagagatgat
    70321 aattgtcggg ccagcaccgc gatcgcggaa gattttactg gcgatgaagt acacggcact
    70381 tttcccccag ccagtgcgtt ggacaaccag cagttttcgt cgttggttca ccaccgcgtc
    70441 tatggcttcc cattgccctt ccctgaaagt agcggtggta tttcctaaca tccgctgtaa
    70501 aagcaattca gctgcgtgct tttccatctt tatctcctgc gatctttagg cagtgattag
    70561 aacggattat tagcaggtaa atttgcgaga gggatcaaaa agatgaagag aggagataag
    70621 agaaatgaat tactgcgagc aagctcgcag taatttgtcg cagtaattaa ctcaacagct
    70681 ggaacgcaat catcccaacg atggcaccga cagtgccgag gatggtttcc atcatggtcc
    70741 aggttttcag cgtttcggct tcggtcgcgc cggtaaattt accgaacaac cagaaaccgg
    70801 cgtcgttaac gtggctgaca acaatcgaac caccagcgat acaaatcgac agcgccgcca
    70861 tttgcgcacc ggagtagttc agttgttcaa taaccggcat caccagtcct accgccgtta
    70921 aacaggctac ggtggcagaa ccctgaatga tgcgcactgc agcggccagc acgaagcagg
    70981 tgatggctat cggcaggccc atgccagtta acgcttcgcc cagtgccgga cctacgccag
    71041 agtcaaccag cacctgtttg aacacgccac ctgcaccaat caccagcaga atgatccccg
    71101 ccggttgcag cgcgtgaccg caaatctcca tcactttgtc ttttggcatg ccctgacgca
    71161 ttgccaggcc ataaatcgct accagacaag caaccagaat cgcggtaaac ggatgaccaa
    71221 taaactcgaa ccattcgtaa gcggtagagc cttctggcac aaaacgcgcg gcaatggttt
    71281 tcagccccac cagcaccagc ggcaacagga tcagcgacag gctgaatccg aaagatggca
    71341 ttttgccttc gccgagatgc ggttcgctga tgtcgtcagg aatatgcaac tccacgtagc
    71401 ggctgatgaa attaccccac agcggcccgg caataatcat tcccggaatt gccgcacaca
    71461 ggccaatcag gatcatccag ccaaaatcgg cgttcatctg cgatgccagc agcattggcg
    71521 ctggccctgg caccaggaac gccgccgctg ccgccacgcc tgcgaataat gggattacca
    71581 gcttcaccag gttcgtaccg gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa
    71641 caatcgccac ttcaaagaac agcggtagcg cacagaccag ccccgcaagg ccgatggcat
    71701 aatgcgcgcg gctgtgaccg aaggatttaa gcattttgac ggcaatctga tcgactgcgc
    71761 cggtttcatg taagatcttg ccaaacatag ctcccagggc gacaaccacc gccaggaagc
    71821 cgagggtgcc tcccatccct ttttccatcg tcgctgcgat tttatcgagc ggcataccgg
    71881 aaaaaaggcc agcccccatg gacaccacca tcaaagccag gaaagcgtgc atacgcgcct
    71941 tcatgactaa aaacagcagc agtaaaacag accctactgc tgttaaaaca agcgttaatg
    72001 tagtcactac ttatttgcct tttttaataa cctcaatggt gcttgccaca acaccttcca
    72061 gcggttgatc gatatccacc accagtacat cagtttcgtc cgcacccggc tcctgcagcg
    72121 tttcaaactg cgtcaccaac atttgggttt taaagaaatg gcctttgcgc gctttcaggc
    72181 ggctttcaat cacgtcaaaa tcgcctttca ggtagatgaa agagagattc ggattacctt
    72241 cacgcagcaa gtcgcgatag tgttttttca atgcagaaca gacgatcagt gacactttat
    72301 tagtgcgctg catagcaaac gcggcgtcgt tcagcgcctg caaccacggt ttgcgatcgt
    72361 cgtcattcag cggttcgcca gacgccattt tttcgatatt gcgccgtgga tggaggaaat
    72421 cgccatcaag aaacgcggca tgaagttgat gcgccacttc actggcgacc gcagatttgc
    72481 cgctgcctga gacgcccatc aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag
    72541 tggtgccccc acaatacaag aattaaaaat gttacgggta actgttatcg gtaacattgt
    72601 ccggccggac aatatcagaa gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta
    72661 cttcaaattt gtgggcttaa atagatccgc ccggtgacaa ggtgaaacct aaatctaaca
    72721 ttttcggtgt cacagattcg ccacgaatac gcgccagcag gcgttcagcg ccaatactgc
    72781 ccatccgctc acgcggcgtc agcacgctcg ccagtcgtgg ctccatcacc tgaccaatgt
    72841 catgaccgtg gaaaccggca atcgccatat cgtcaggaac ttttaacccc agacgctgac
    72901 attcaaacgc cgcgccgacc gccagatcat cattagtaca gaacacgcca tccagctgcg
    72961 gatattcccg ccgcgcctgg cgaatcagtt caataccgga agagtaagaa gaagattgct
    73021 caaccatcac gctatacggc accaggcctg catccagcat cgcctgttcg tatcccttct
    73081 gtttgatgat agtacgttcg tcgagacgtg cgccgagata ggcaatgtgg cgatgcccgc
    73141 gcgcaataat ggcggtagtc atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga
    73201 tatcgaggca cggcgactgg ctgtccatca actccaccac cgggatcccc gccacttcaa
    73261 tcatctttaa ggtgcgcggc gtgtgggtac gttcggtgag gatcaggccg tcgatattcc
    73321 aggacagcat ggattcgaga cgttcttgct ccatttccgg tttataaccg tagtgcgcca
    73381 gcatcgtctg ataaccgtgc gcgtcggtga cgctttcgat tccgcgtaat acttccgcga
    73441 aaacctggtt ggtgagagaa ggtaacagga cgccaatcgc ccggctggta gcgttagaga
    73501 ggatatcggg cgcacgattg ggaatatagc ccagttcatc aagagcggcg gcaatcttgc
    73561 cgcgtagagc gacggaaacc tgctccgggt tgcgtaaaaa acggctgacc gtcattttgg
    73621 tcacgcctac acggtcagcc acatcctgaa gtacgggtct tttctttttc atcgtcctga
    73681 aggtacaaaa gagatagatt ctcttagttt aacacggacg catcagaacc ttcccgggga
    73741 aaacagtgct tgtttacaat tattagatat acactaaatc aattgcatcg cattgtgcta
    73801 acgacgcggc aaaaccccgg aagcgtgcat cagcacgcga ccggagcgag cgaggaagcc
    73861 tgcaaaaagg caggcttcaa agatgccgtt gagttttaaa ccggcggcag atcgaacagt
    73921 aacacttcgc tgtcgctatc cgcatggatg gagattgcct gctcatccca gattgccaga
    73981 ccatcgctgg tcgaggcttt cacgccgtta atggtgacat tgcctttcac cacctggatc
    74041 cagacgcggc gttcagcggc aatctgatgc accgactgct catctttcag caacgcccaa
    74101 cgatacagtt ccatatcctg atgcactttc agcgaaccat ctcgcgcatc cggcgagagc
    74161 accagctgtt tgccctgcac ggcatcgaag cgacgctgtt cataacgcgg cgtaatgcca
    74221 ttttcttcag gcatgatcca gatctgatac agatgcagac gctcggtgct gtttgggttg
    74281 tactctgagt gacgaatacc cgtacccgca ctcataatct ggaactcacc cgccggaacc
    74341 tgctctttat tgcccatgct gtcctgatgc tcaacagtac cttccagcac gtaggtcaaa
    74401 atttccatat ctttatgcgg gtgagtgccg aagccctgcc ctgcttcaat cacgtcgtcg
    74461 ttaatcacgc gcagcgcgga gaagcccata aagttcggat cgtaatagtt ggcaaaagag
    74521 aaagtatgcc aggagtccag ccagccatga tttgcatgac cacgttcatt tgctttgcgg
    74581 aagtagatca tttgtattcg ccccctgaat gatttcgatg ggcttagtgt ggacccgatc
    74641 tgcctgggat gatagagggt gaaaattgac ccctctgttc aaaaaaattg aactattcga
    74701 ggggccatca aacttactta gcgagagtta ctgtggaggg agaggcttgc tcaaatccgc
    74761 gttcaaggat ttccagattg gtaagaactt cagattcctt gacgtaattt ggcgcaccgt
    74821 gggtgatggt ttgatacaac gcatcataaa cgcgcccgta atcgcccatc tccggcttca
    74881 tctcttctct gaccgtcacg ccctcgtcat tgacatactc cagcacaccg accgaatcat
    74941 ccgctgcgaa tcccggttcg cccggcataa tattagcctt caggctggtt tcctgctggt
    75001 cgataccgta tttaataaac gaacctttct taccgtgaac gataaatttc ggataatcga
    75061 ttttcaccag atggctggtt ttgacgatgg ctttcaggtc gccataaaac agttgcgctt
    75121 caaaggtgtc gtcaggattg gctttattac gcaggctgcg gatgtcataa gcgacgtgat
    75181 ccgggcgacc gaacagagaa ataatctggt ccatcgtatg cacaccaagg ccataaaacg
    75241 cgccatcctg cggcagccca ggtttggttt ctgccaccgg gcggtaatag tcaaaatggc
    75301 tttccacttc aacaatctct cccaacttgc cactttcaat cgcttttttc gctgtcagga
    75361 agcaggagtc aaagcgacga ttctgatacg gcgtgacggt cagccctttg cttttcgcca
    75421 acgcaaatag ctccttcgcc tgcgcaagtg tcggagtgaa cggtttttcg accagcacat
    75481 ttttcccggc ttccagcgcg cgtttcgcgt actcgaaatg gctgtccgcg tgggtgcaga
    75541 caacaaccag cttaacatcg ggatcgttta gtacttcgtc gagatcgctg gtgaaatgga
    75601 tatgggaata aatgggagcc tgttcttccg gcttcgcatg gcgacgaaaa atatgcgcga
    75661 catgccagct atccttgcgg ttaagtacat acggcagatg gtaacgggtg gtgcttttgc
    75721 cgaagccaat aaaggcgcag ttgatgacca tgatttcgtc ctttttaagg tggttattta
    75781 caccttagcg caaagcggac gtggttccta ctggagtgcg cataacgaac acaagcactc
    75841 ccgtggataa attgagaacg aaagatcaaa aaaaaagcca gcacccggct ggctaagtaa
    75901 tactggaagc aatgtgagca atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc
    75961 gcgagacaat aataatcatt ctcattcgca cttgtccaac actttttgca aaaaaatgca
    76021 tttgactcgc atttgaaagt caatgatgtt gaaagggaca tttaccccaa agaggacaaa
    76081 ggaatgagtg agatagtaat acgccacgca gaaacacggg attacgaggc catcaggcag
    76141 attcacgccc agccggaggt gtattgcaac acactacagg tgcctcatcc ttccgatcat
    76201 atgtggcagg agcgactcgc cgatcgtccc ggcatcaagc aactcgtcgc ctgtattgat
    76261 ggagacgtcg tgggccatct caccattgac gtgcaacagc gcccacgccg cagtcatgtt
    76321 gccgattttg gtatctgtgt cgactctcgt tggaagaacc gcggcgtcgc cagcgccctg
    76381 atgcgagaga tgattgaaat gtgcgacaac tggttgcggg tagatcgcat tgaactaacc
    76441 gtgtttgtcg ataacgcccc ggcaattaag gtctataaaa aatacggctt tgaaattgaa
    76501 gggactggta agaagtacgc attgcgtaat ggtgaatatg tcgatgcata ttatatggcg
    76561 cgggtgaagt aagatagtgc cctttttctg agatggaaaa agggtgtcat tcaaaatcga
    76621 cataccttcc tttaaggtat ttatttgccc aatacataaa ttgagattta actcataatt
    76681 acttcctaaa gtgtaatatt ttatttttta atatatacgc ctacaatttc ctggagtaaa
    76741 taaataacaa ttaacaagca taatattgcc attgataaaa tagcatgcca taaaaggact
    76801 tttcagggat gagtaatatt gtttacctga cagtaacggg agaacaacaa ggaagcatct
    76861 ccgcaggttg tgggacttct gagtctacag gtaatcgctg gcagagcggg catgaggatg
    76921 aaatatt
//
LOCUS       DAZGSP010000007        74014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZGSP010000007 DAZGSP010000000
VERSION     DAZGSP010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 74014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.007"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..752)
                     /locus_tag="ACHK74_000541"
     CDS             complement(<1..752)
                     /locus_tag="ACHK74_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP0219425.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKGLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRRVKFSNNWQKQKRKIQRLHSRIANIRRDYL"
     gene            822..1184
                     /gene="tnpA"
                     /locus_tag="ACHK74_000542"
                     /pseudo
     CDS             822..1184
                     /gene="tnpA"
                     /locus_tag="ACHK74_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1248..1541)
                     /locus_tag="ACHK74_000543"
                     /pseudo
     CDS             complement(1248..1541)
                     /locus_tag="ACHK74_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            1813..2607
                     /gene="yjfZ"
                     /locus_tag="ACHK74_000544"
     CDS             1813..2607
                     /gene="yjfZ"
                     /locus_tag="ACHK74_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFP0219426.1"
                     /translation="MTLPTTIYSFPAYSPHFSSTDKPVKLKFHQYARATLLPNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKY
                     NKAHAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNS
                     AAIYSGLGFERVPQDRDIRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(2678..3127)
                     /gene="rplI"
                     /locus_tag="ACHK74_000545"
     CDS             complement(2678..3127)
                     /gene="rplI"
                     /locus_tag="ACHK74_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFP0219427.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(3169..3396)
                     /gene="rpsR"
                     /locus_tag="ACHK74_000546"
     CDS             complement(3169..3396)
                     /gene="rpsR"
                     /locus_tag="ACHK74_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFP0219428.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(3401..3715)
                     /gene="priB"
                     /locus_tag="ACHK74_000547"
     CDS             complement(3401..3715)
                     /gene="priB"
                     /locus_tag="ACHK74_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFP0219429.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(3722..4117)
                     /gene="rpsF"
                     /locus_tag="ACHK74_000548"
     CDS             complement(3722..4117)
                     /gene="rpsF"
                     /locus_tag="ACHK74_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFP0219430.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            4444..4719
                     /gene="yjfY"
                     /locus_tag="ACHK74_000549"
     CDS             4444..4719
                     /gene="yjfY"
                     /locus_tag="ACHK74_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFP0219431.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(4794..5345)
                     /locus_tag="ACHK74_000550"
     CDS             complement(4794..5345)
                     /locus_tag="ACHK74_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFP0219432.1"
                     /translation="MAIKGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCVV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(5440..6126)
                     /gene="ulaF"
                     /locus_tag="ACHK74_000551"
     CDS             complement(5440..6126)
                     /gene="ulaF"
                     /locus_tag="ACHK74_000551"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFP0219433.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(6126..6980)
                     /gene="ulaE"
                     /locus_tag="ACHK74_000552"
     CDS             complement(6126..6980)
                     /gene="ulaE"
                     /locus_tag="ACHK74_000552"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFP0219434.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(6990..7640)
                     /gene="ulaD"
                     /locus_tag="ACHK74_000553"
     CDS             complement(6990..7640)
                     /gene="ulaD"
                     /locus_tag="ACHK74_000553"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP0219435.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(7654..8118)
                     /gene="ulaC"
                     /locus_tag="ACHK74_000554"
     CDS             complement(7654..8118)
                     /gene="ulaC"
                     /locus_tag="ACHK74_000554"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFP0219436.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(8128..8433)
                     /gene="ulaB"
                     /locus_tag="ACHK74_000555"
     CDS             complement(8128..8433)
                     /gene="ulaB"
                     /locus_tag="ACHK74_000555"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFP0219437.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(8449..9846)
                     /gene="ulaA"
                     /locus_tag="ACHK74_000556"
     CDS             complement(8449..9846)
                     /gene="ulaA"
                     /locus_tag="ACHK74_000556"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP0219438.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            10201..11265
                     /gene="ulaG"
                     /locus_tag="ACHK74_000557"
     CDS             10201..11265
                     /gene="ulaG"
                     /locus_tag="ACHK74_000557"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFP0219439.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            11373..12128
                     /gene="ulaR"
                     /locus_tag="ACHK74_000558"
     CDS             11373..12128
                     /gene="ulaR"
                     /locus_tag="ACHK74_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFP0219440.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(12125..12874)
                     /gene="yjfP"
                     /locus_tag="ACHK74_000559"
     CDS             complement(12125..12874)
                     /gene="yjfP"
                     /locus_tag="ACHK74_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP0219441.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSCLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            13056..13385
                     /gene="bsmA"
                     /locus_tag="ACHK74_000560"
     CDS             13056..13385
                     /gene="bsmA"
                     /locus_tag="ACHK74_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFP0219442.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            13534..13809
                     /gene="yjfN"
                     /locus_tag="ACHK74_000561"
     CDS             13534..13809
                     /gene="yjfN"
                     /locus_tag="ACHK74_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFP0219443.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(13926..15551)
                     /gene="aidB"
                     /locus_tag="ACHK74_000562"
     CDS             complement(13926..15551)
                     /gene="aidB"
                     /locus_tag="ACHK74_000562"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFP0219444.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(15635..16798)
                     /gene="yjfC"
                     /locus_tag="ACHK74_000563"
     CDS             complement(15635..16798)
                     /gene="yjfC"
                     /locus_tag="ACHK74_000563"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP0219445.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(16801..17439)
                     /gene="yjfM"
                     /locus_tag="ACHK74_000564"
     CDS             complement(16801..17439)
                     /gene="yjfM"
                     /locus_tag="ACHK74_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFP0219446.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVISGFLLSRVIRKDRDEQFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(17449..17847)
                     /gene="yjfL"
                     /locus_tag="ACHK74_000565"
     CDS             complement(17449..17847)
                     /gene="yjfL"
                     /locus_tag="ACHK74_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFP0219447.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(17865..18524)
                     /gene="yjfK"
                     /locus_tag="ACHK74_000566"
     CDS             complement(17865..18524)
                     /gene="yjfK"
                     /locus_tag="ACHK74_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFP0219448.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(18575..19273)
                     /gene="yjfJ"
                     /locus_tag="ACHK74_000567"
     CDS             complement(18575..19273)
                     /gene="yjfJ"
                     /locus_tag="ACHK74_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFP0219449.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(19292..19693)
                     /gene="yjfI"
                     /locus_tag="ACHK74_000568"
     CDS             complement(19292..19693)
                     /gene="yjfI"
                     /locus_tag="ACHK74_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFP0219450.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(19820..20551)
                     /gene="rlmB"
                     /locus_tag="ACHK74_000569"
     CDS             complement(19820..20551)
                     /gene="rlmB"
                     /locus_tag="ACHK74_000569"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFP0219451.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(20731..23172)
                     /gene="rnr"
                     /locus_tag="ACHK74_000570"
     CDS             complement(20731..23172)
                     /gene="rnr"
                     /locus_tag="ACHK74_000570"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFP0219452.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLSKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(23211..23636)
                     /gene="nsrR"
                     /locus_tag="ACHK74_000571"
     CDS             complement(23211..23636)
                     /gene="nsrR"
                     /locus_tag="ACHK74_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFP0219453.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(23841..25139)
                     /gene="purA"
                     /locus_tag="ACHK74_000572"
     CDS             complement(23841..25139)
                     /gene="purA"
                     /locus_tag="ACHK74_000572"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFP0219454.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(25243..25440)
                     /gene="yjeT"
                     /locus_tag="ACHK74_000573"
     CDS             complement(25243..25440)
                     /gene="yjeT"
                     /locus_tag="ACHK74_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFP0219455.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(25522..26526)
                     /gene="hflC"
                     /locus_tag="ACHK74_000574"
     CDS             complement(25522..26526)
                     /gene="hflC"
                     /locus_tag="ACHK74_000574"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFP0219456.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(26529..27788)
                     /gene="hflK"
                     /locus_tag="ACHK74_000575"
     CDS             complement(26529..27788)
                     /gene="hflK"
                     /locus_tag="ACHK74_000575"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFP0219457.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGTSNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(27874..29154)
                     /gene="hflX"
                     /locus_tag="ACHK74_000576"
     CDS             complement(27874..29154)
                     /gene="hflX"
                     /locus_tag="ACHK74_000576"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFP0219458.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(29231..29539)
                     /gene="hfq"
                     /locus_tag="ACHK74_000577"
     CDS             complement(29231..29539)
                     /gene="hfq"
                     /locus_tag="ACHK74_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFP0219459.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(29625..30575)
                     /gene="miaA"
                     /locus_tag="ACHK74_000578"
     CDS             complement(29625..30575)
                     /gene="miaA"
                     /locus_tag="ACHK74_000578"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFP0219460.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(30568..32415)
                     /gene="mutL"
                     /locus_tag="ACHK74_000579"
     CDS             complement(30568..32415)
                     /gene="mutL"
                     /locus_tag="ACHK74_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFP0219461.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(32425..33762)
                     /gene="amiB"
                     /locus_tag="ACHK74_000580"
     CDS             complement(32425..33762)
                     /gene="amiB"
                     /locus_tag="ACHK74_000580"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFP0219462.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(33781..34242)
                     /gene="tsaE"
                     /locus_tag="ACHK74_000581"
     CDS             complement(33781..34242)
                     /gene="tsaE"
                     /locus_tag="ACHK74_000581"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFP0219463.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(34214..35761)
                     /gene="nnr"
                     /locus_tag="ACHK74_000582"
     CDS             complement(34214..35761)
                     /gene="nnr"
                     /locus_tag="ACHK74_000582"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFP0219464.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            35760..36899
                     /gene="queG"
                     /locus_tag="ACHK74_000583"
     CDS             35760..36899
                     /gene="queG"
                     /locus_tag="ACHK74_000583"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFP0219465.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(36882..36935)
                     /locus_tag="ACHK74_000584"
     CDS             complement(36882..36935)
                     /locus_tag="ACHK74_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219466.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(37169..37244)
                     /locus_tag="ACHK74_000585"
     tRNA            complement(37169..37244)
                     /locus_tag="ACHK74_000585"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37209..37211),aa:Gly,seq:gcc)
     gene            complement(37280..37355)
                     /locus_tag="ACHK74_000586"
     tRNA            complement(37280..37355)
                     /locus_tag="ACHK74_000586"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37320..37322),aa:Gly,seq:gcc)
     gene            complement(37392..37467)
                     /locus_tag="ACHK74_000587"
     tRNA            complement(37392..37467)
                     /locus_tag="ACHK74_000587"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37432..37434),aa:Gly,seq:gcc)
     gene            complement(37678..38223)
                     /gene="orn"
                     /locus_tag="ACHK74_000588"
     CDS             complement(37678..38223)
                     /gene="orn"
                     /locus_tag="ACHK74_000588"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFP0219467.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            38318..39370
                     /gene="rsgA"
                     /locus_tag="ACHK74_000589"
     CDS             38318..39370
                     /gene="rsgA"
                     /locus_tag="ACHK74_000589"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFP0219468.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            39467..40435
                     /gene="asd"
                     /locus_tag="ACHK74_000590"
     CDS             39467..40435
                     /gene="asd"
                     /locus_tag="ACHK74_000590"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFP0219469.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGAFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            40457..43780
                     /gene="mscM"
                     /locus_tag="ACHK74_000591"
     CDS             40457..43780
                     /gene="mscM"
                     /locus_tag="ACHK74_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFP0219470.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VIEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFVNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(43931..45432)
                     /gene="yjeM"
                     /locus_tag="ACHK74_000592"
                     /pseudo
     CDS             complement(43931..45432)
                     /gene="yjeM"
                     /locus_tag="ACHK74_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(45651..46628)
                     /gene="epmA"
                     /locus_tag="ACHK74_000593"
     CDS             complement(45651..46628)
                     /gene="epmA"
                     /locus_tag="ACHK74_000593"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFP0219471.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            46953..48761
                     /gene="frdA"
                     /locus_tag="ACHK74_000594"
     CDS             46953..48761
                     /gene="frdA"
                     /locus_tag="ACHK74_000594"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFP0219472.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            48754..49488
                     /gene="frdB"
                     /locus_tag="ACHK74_000595"
     CDS             48754..49488
                     /gene="frdB"
                     /locus_tag="ACHK74_000595"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFP0219473.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            49499..49894
                     /gene="frdC"
                     /locus_tag="ACHK74_000596"
     CDS             49499..49894
                     /gene="frdC"
                     /locus_tag="ACHK74_000596"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFP0219474.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            49905..50264
                     /gene="frdD"
                     /locus_tag="ACHK74_000597"
     CDS             49905..50264
                     /gene="frdD"
                     /locus_tag="ACHK74_000597"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFP0219475.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            50327..51460
                     /gene="ampC"
                     /locus_tag="ACHK74_000598"
     CDS             50327..51460
                     /gene="ampC"
                     /locus_tag="ACHK74_000598"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP0219476.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            51549..52082
                     /gene="blc"
                     /locus_tag="ACHK74_000599"
     CDS             51549..52082
                     /gene="blc"
                     /locus_tag="ACHK74_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP0219477.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(52079..52396)
                     /gene="sugE"
                     /locus_tag="ACHK74_000600"
     CDS             complement(52079..52396)
                     /gene="sugE"
                     /locus_tag="ACHK74_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFP0219478.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(52578..52724)
                     /gene="ecnB"
                     /locus_tag="ACHK74_000601"
     CDS             complement(52578..52724)
                     /gene="ecnB"
                     /locus_tag="ACHK74_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFP0219479.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(52835..52960)
                     /gene="ecnA"
                     /locus_tag="ACHK74_000602"
     CDS             complement(52835..52960)
                     /gene="ecnA"
                     /locus_tag="ACHK74_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFP0219480.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(53012..53578)
                     /gene="efp"
                     /locus_tag="ACHK74_000603"
     CDS             complement(53012..53578)
                     /gene="efp"
                     /locus_tag="ACHK74_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP0219481.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            53620..54648
                     /gene="epmB"
                     /locus_tag="ACHK74_000604"
     CDS             53620..54648
                     /gene="epmB"
                     /locus_tag="ACHK74_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFP0219482.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVVSGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            55038..55907
                     /gene="yjeJ"
                     /locus_tag="ACHK74_000605"
     CDS             55038..55907
                     /gene="yjeJ"
                     /locus_tag="ACHK74_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFP0219483.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(56110..56463)
                     /gene="yjeI"
                     /locus_tag="ACHK74_000606"
     CDS             complement(56110..56463)
                     /gene="yjeI"
                     /locus_tag="ACHK74_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFP0219484.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(56601..58247)
                     /gene="groL"
                     /locus_tag="ACHK74_000607"
     CDS             complement(56601..58247)
                     /gene="groL"
                     /locus_tag="ACHK74_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP0219485.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(58291..58584)
                     /gene="groS"
                     /locus_tag="ACHK74_000608"
     CDS             complement(58291..58584)
                     /gene="groS"
                     /locus_tag="ACHK74_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP0219486.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            58860..60116
                     /gene="yjeH"
                     /locus_tag="ACHK74_000609"
     CDS             58860..60116
                     /gene="yjeH"
                     /locus_tag="ACHK74_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFP0219487.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            complement(60132..60608)
                     /gene="fxsA"
                     /locus_tag="ACHK74_000610"
     CDS             complement(60132..60608)
                     /gene="fxsA"
                     /locus_tag="ACHK74_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFP0219488.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            60945..62381
                     /gene="aspA"
                     /locus_tag="ACHK74_000611"
     CDS             60945..62381
                     /gene="aspA"
                     /locus_tag="ACHK74_000611"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFP0219489.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            62499..63800
                     /gene="dcuA"
                     /locus_tag="ACHK74_000612"
     CDS             62499..63800
                     /gene="dcuA"
                     /locus_tag="ACHK74_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFP0219490.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            63916..64254
                     /gene="cutA"
                     /locus_tag="ACHK74_000613"
     CDS             63916..64254
                     /gene="cutA"
                     /locus_tag="ACHK74_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFP0219491.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            64230..65927
                     /gene="dsbD"
                     /locus_tag="ACHK74_000614"
     CDS             64230..65927
                     /gene="dsbD"
                     /locus_tag="ACHK74_000614"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFP0219492.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAAPQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLKAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            65964..66539
                     /gene="yjdC"
                     /locus_tag="ACHK74_000615"
     CDS             65964..66539
                     /gene="yjdC"
                     /locus_tag="ACHK74_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0219493.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            66646..66721
                     /locus_tag="ACHK74_000616"
     tRNA            66646..66721
                     /locus_tag="ACHK74_000616"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:66679..66681,aa:Phe,seq:gaa)
     gene            66898..68082
                     /locus_tag="ACHK74_000617"
     CDS             66898..68082
                     /locus_tag="ACHK74_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0219494.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQVTDILQQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYIDGRREMMQWYADYIDSLSEQ
                     VS"
     gene            68411..69454
                     /locus_tag="ACHK74_000618"
     CDS             68411..69454
                     /locus_tag="ACHK74_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP0219495.1"
                     /translation="MNDRLRFEVNDNQGCFVFPDAWFGSLLDEFDELIDAYDTDEISE
                     TSYINKLRRLARQEPDFIDVHAYLAYAFLEQNAPRKALNAALKGLAAGNRLIPESFSG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAALLTDKILAYNPEDNQGARWLLGPEL
                     LRTGDHEQARHVLKAHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNFSGGPDTAEDYYATYHPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQEKLRERIDETLSEKIVQKCRNMNGEYVWPWILPF
                     SAAGMKHTGIQYQ"
     gene            69597..69826
                     /locus_tag="ACHK74_000619"
                     /pseudo
     CDS             69597..69826
                     /locus_tag="ACHK74_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(69813..71315)
                     /locus_tag="ACHK74_000620"
     CDS             complement(69813..71315)
                     /locus_tag="ACHK74_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP0219496.1"
                     /translation="MNIRKLFCPDNTPRILLFLFFFVISVITTIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTADEAAEF
                     TGMIPVYHFLVSAAILVFMVIFWRTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVG
                     TTDTLREMQRYEQLSQHRADSWKILPGAPLYDTIVIVTGESVRRDYMSVYGYPVPTTP
                     WLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFL
                     HMIGSHPNPCDRLNSWPNHYLEQYPRKIACYLASISKLDNFLGQLDGILRRHSRHFAM
                     LYFSDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSVSRKISARHFAGIFQ
                     WLTDIRTENIPPFNPLTDEDNEPVMVFNGERNVPADSLKPQPLILPVRGK"
     gene            complement(71477..72223)
                     /locus_tag="ACHK74_000621"
     CDS             complement(71477..72223)
                     /locus_tag="ACHK74_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFP0219497.1"
                     /translation="MKKIIAVSALAVVGMFSGQALADRGKTGFYMTGKAGASVVTQTD
                     QRFCQDFGDDVYKYKGGDKNDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGAADSRY
                     TLDTWYSPMVGGGREDTQNRLSVNTLMVNTYYDFRNSSAFTPWVSVGLGYARIHHKAT
                     YTDTSWNKSGEVSDFSALHYPGYDNNFAWSFGAGVRYDVTPDIALDLSYRYLDAGDAS
                     VSYKDEWGDKYKSEVDVKSHDIMLGVTYNF"
     gene            73013..>74014
                     /gene="subA"
                     /locus_tag="ACHK74_000622"
     CDS             73013..>74014
                     /gene="subA"
                     /locus_tag="ACHK74_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFP0219498.1"
                     /translation="MLKNLRLHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKEPEALYIHGTAMASLIASRHG
                     VYGVYPHALISSRRVIPDGVQDSWLRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWSELLSRMGRNNERLIVAAVGNDGADIRKLSAQQRIWPAAYHPVSSVNKKQDPVIRV
                     AALAQYRKGETPVLHGGGVTGSRFGNGWVDIAAPGQNITFLRPDGKTGIGSGTSEATA
                     IVSGVLAAMVSCNPRATATELKRTLLESADKYPSLADKVTEGRVLNAEKAISMFCKKN
                     "
BASE COUNT        18343 a        19603 c        18179 g        17889 t
ORIGIN      
        1 tgaaggtagt ccctgcggat atttgcgata cgggaatgca gtcgctgtat tttgcgtttc
       61 tgcttctgcc agttgttgct gaatttgacc ctgcggctta actgtcgctg aagtctcgcc
      121 agcgtcttct ggtttttctg aaaactgttt acaggctcaa agactgtgcc atctgacagc
      181 gtggcgagtt tagccacgcc agcatccagc ccgaccattg atgctgaagg gtgaaccgga
      241 gtggatactt cactttctgt ctgaatactg atgtaccact taccgcagga ctggctgaca
      301 gtgacatttt tcacaactcc cgtgacctga cggctattcc ggtagcgcat ccagccaagt
      361 ttcggcagaa aaatacggct gttttcctga tcgagcttaa cgccctgcgg gtagcggaat
      421 gcatcattct gtccccgctt tttgaatcgg ggaaaagcag cccgcttctg gaagaagttt
      481 ttgtaagccc gctcaaggcc tttcagtgac tgttgcaatg gctgtgaggg cgaatcttta
      541 agccattgcg tttcagtggc gtttttccac tcaaccagcc aggaagccat tttcccgtaa
      601 gggatgtatt tattaccggc ctcatgattc tcattctgaa gcgccagagc acgattgaaa
      661 acgaaacgac atgcgcctgc gaagcgcctc atttcacgct cctgttgacc accgggtctt
      721 aactggaatt taaatgcctg taatcgtttc acaggccaaa tgatactttg gtctatgaat
      781 aaagaaaccg atatccgacg gggaagcatt gtgttttcct gatgcatgtt cacctggtct
      841 ttgtcaccag ataccgacgt cagatttttg actatgacgc gacagaaaaa ctacgcactt
      901 acttttcaaa tgtatgtgct gattttgaag ctgaactggt tgaaatggat ggcgaaccag
      961 atcacgtcca tttgttaatt aactatcctc ccaaactggc gatatccagt ctggtaaaca
     1021 gcctcaaagg cgtatcgggt aggttactgc gacgagatcg accagatatt gcagtcaggt
     1081 attactacaa aggcgttttg tggagtcctg gctattttgc caatagctgc ggaggtgcgc
     1141 caatatccgt catccgccaa tacattgaac aacagcaaac acctggtcag gtggaaaacc
     1201 gcgccttata tccccgccct gaaggacggg gttttacggc gcaccagata aaccaggcca
     1261 agcgtatgct ggttgatcaa ataatatccc ccaagcgcag caatattaat attcacctgc
     1321 aatgggtcga tacctggctt aaaaatattg tatttcttcc cttcatccaa taaggattcc
     1381 attaactgca agtgcgcatg gttaatttct aagagacgct gtgattttgc ataatgcaca
     1441 cctttacttt gattttcact atgcacaatt tttaaaaacc aagggttagc aatataataa
     1501 tcccatgtaa aatcaataat tttctcaatg gctttttgtg gctggttaac cagatgatta
     1561 acaaacgcac cgataccaac agcgtagaat aattacatac cgcacaacaa gaatgtatgg
     1621 tggaaggcgt ggaagtcatt ttctccccgg gggcatcgtt atatccataa tgagcgattt
     1681 ttgatattaa tatctttcac catccgctat tttcactact tcagaaaaca aacttatttt
     1741 tattaaaatc gattcttttt ctgtgaacat cagctgcgat atttataaaa acattaaaca
     1801 ggtaacatta caatgacatt accaacgact atttattcat ttcctgcata ttcaccccat
     1861 ttttcgtcca ccgacaaacc agtaaaatta aaatttcatc aatatgcacg tgctacatta
     1921 ctgccaaatc gtggaagaga tcataattgc gatggcagac gtactgttga gatccacaaa
     1981 ctagatctct ctgactggca agcattcaat aagttagcaa cgaagtgtaa tgcttacgat
     2041 ggagtaacaa tgaatggtga taattctttt ggatggaacc atgaagctac attagataat
     2101 attcacgccc agaaatacaa taaagctcac gcgggtgcga ggttaaccgc agaattgaaa
     2161 tatttattgc aagatgtcga atccttcgaa cccaacagta aatacacaat acacgaagtg
     2221 gttttgggac ctggttatgg tactccagac tataccggcc aaacgatagg atacgttgta
     2281 acccttcctg cccaaatgcc taattgctgg tcatctgaac taccaacaat cgatctttat
     2341 atcgaccaac ttcgcactgt tacaggcgtt tctaatgccc tcggattcat cattgctgca
     2401 ttgctaaacg cgtattctga tttaccccat gatttgaaaa ttggtctacg ttccttatca
     2461 aattcagcag caatctactc gggtttaggt ttcgagcgag tgccgcagga ccgagacata
     2521 aggagtgatc gtatgcatct aacgccagca aaccatcctg acttatggac acaagagaat
     2581 ggtgaatgga tctacttgcg aaactaatac ctgacgaaaa atagaaagca aaacgccgac
     2641 caatggtcgg cgtttttacg tctcgttgaa taacgaatta ttcagctact acgtttacga
     2701 tcactttcgc gaatacttcg ctgtgaacct ggaagctcac ttcgtgttcg ccagtggtac
     2761 gcagaacgcc gttcggcaga cgaacttcgc tcttagccac ttcaacgcca gctgcagtta
     2821 cagcgtcagc gatgtcgcga gtaccgatgg aaccgaacag tttaccttcg tcgccagctt
     2881 tagacgcgat ggtaacagtt tccagtgcat tgattttctc agcgcgagca ttagcagctg
     2941 ccagaacttc agccagttta gcttccagtt cagcgcgacg tgcttcgaag aattcaatgt
     3001 ttttcttggt agctggaaca gctttaccct gcggtaccag gaagttacga gcatagcccg
     3061 ctttaacgtt tacctgatca cccaggctac ccaggtttgc tactttatca agcagaataa
     3121 cttgcattac cttatcctct caaagtcgta ttaatggacc gtgaccgatt actgatggcg
     3181 atcagtgtac ggcagcaggg acaggtagcg agcgcgtttg atagcgcgag ccagctgacg
     3241 ctggtatttt gcacgggtac cggtgatacg gcttgggaca atcttaccgc tttcggtgat
     3301 gtagtttttc agcgtagcga tatctttata gtcgatctct tgaacgcctt ccgcggtgaa
     3361 acggcagaac ttgcgacgac ggaaataacg tgccatatgg ctagtctcca gaatctatca
     3421 attcaatctg ctcggcatgc aaaaccattt tgctcagtcc gttctttgcc ttgtggcatg
     3481 aaatgaaccc ctgaacggtt atgcgactgc cgaccgttat actgtgagta atggcctggt
     3541 tttcgtgtcc gctaacaata acgggcattt ggcaccacgc ctgccggtga aaaccggctt
     3601 cctcctgcac agaacgatgc tcaagcacga actggcagtg aggaattcct gatggactga
     3661 cctttcgaag gggagtcctg cacacggtgc cggacaacac cagacggttg gtcatcagaa
     3721 attactcttc agaatcccca gcttcagcat catcagcggt ttcgtttgcg aaatcatcgc
     3781 gacgctcacg gcgctcgtct ttcgctttaa ccatcggaga tgcttcggta acagcgtgct
     3841 tggtacgcat aaccatgctg cggataacgg catcgttgaa gcggaaggta gtttccagct
     3901 catcgatcac ttcctgcgga gcttcaacgt tcatcagaac gtagtgtgct ttgtgcagtt
     3961 tgttgatcgg gtaagccagc tgacggcggc cccagtcttc cagacggtgg atcttgcctt
     4021 ctgcaccagt gatggcagca gtgtagcgct cgatcatgcc cggaacctgt tcgctctggt
     4081 caggatggac cataaaaacg atttcgtaat gacgcatcga attgctcctt acggattatt
     4141 cagcctcctg tctgggtcag ccgaatcccg gggaggcaag gaacgtgtta aaggtcggct
     4201 gaaaaatgac gcgttatagt acttgcatgc cccgctaaac tcaaggccag ccgacaaata
     4261 aatctcatct ttcgctcaaa agaactaact ccctgatttg aagttcatca cattccgtca
     4321 cagtgttttt tcgaacaact gcatcagaaa tggtcaccat caacttcttt atcattgatt
     4381 aaaattaaat cagaagatca tcacagagga gagcacacaa agtcagccgt cgaggaggta
     4441 tctatgttca gtcgagtttt agcccttctg gctgtgcttt tgctaagtgc aaatacatgg
     4501 gcagccattg aaattaataa ccaccaggcc agaaacatgg atgatgtgca aagcttaggc
     4561 gtgatttata tcaatcataa tttcgccact gaaagcgaag cgcgtcaggc attaaatgaa
     4621 gagacagatg cgcagggcgc aacgtactac cacgtaattc tgatgcggga accggggagt
     4681 aacggcaata tgcacgccag cgcggatatt tatcgctagc accaggtata accaacgaaa
     4741 cattgccata gtttgctttg cccccttcgc agggggcttt tttatacggt tattcaccga
     4801 attgcgactt ccggatctgg ctcatgagta atgcgattac gtaagtcgcg gcgcataatc
     4861 tctattatcc agaaccagaa aatatgcccc aggatttcag acgcatattc gtcaaaaggc
     4921 aattgagtga gcggcggcgt taaattcagc gccgggcaaa atataccgtg gactgccact
     4981 gtcacaataa gccctgctaa tatcccctgc cataatttca ctttcggaaa aatttccgcg
     5041 acaacacaat accctatcgc aaaaaccagt gagaagatga tatgcgtcac catcaccggg
     5101 ttgataatat gttccgagaa tgtgtacaca gtttgggttg ggtcgatacc taaataatct
     5161 cgtagaaaga tatacggcgg gttaaattca tcgcgaccac cggaaaatgt gcgcggtggc
     5221 aaggggactt ccgctcccca tttaacaaat gctgaaataa tgcctgaaag tacacccaca
     5281 agtaacgcaa cgccatagcg gcgacgctgc ggatccgttc tgacaaacaa tcctttgata
     5341 gccataaagc acctgatgtt gatgatttta ttaactggag aggccccgga atcagaccaa
     5401 ttacatcccg caggaggtaa taattccggg gcgcactgct tacttctgcc cgtaataagc
     5461 gttaggaccg tgtttacgca tgaagtgttt attcatgagg tagctgtcga tgtgattcag
     5521 ttgtgggtta atgccgcggg caatccacgc cattttcgcc acttcttcca tcaccaccgc
     5581 gttatgcacc gcgtcgtggg catctttccc ccaggcgaac ggtccgtgct gatacaccac
     5641 aattcccggc gtatgcagcg gctcggcgtt gcccagcgtt tcgataatca ctttgccagt
     5701 gttcagttca tactcaccct gcacctcttc ttcgcttaat ccacgcgtac acgggatgtc
     5761 gccaaagaag tagtcggcat gcgtagtgcc taacgccggg atcgccagcc ccgcctgcgc
     5821 ccatgcggta gcatgagtgg agtgggtatg gacaatgccg ccaagcgacg gataacgacg
     5881 gtagagttca agatgcgtcg cggtgtcgga agatgggcga tactcccctt ccaccacctt
     5941 accgctcata tcgaccacca ccatatcgtc tgctttcatg gtttcgtagg cgacgccgct
     6001 gggcttgatc accaccagcc cgcgttcgcg gtcgatagcg ctgacgttgc cccaggtaaa
     6061 ggtcaccagc ccgtagcgcg gcagatccat gttggcttca aatacctgct gttttagctt
     6121 ttgcattatg ccgcctccac catgcccgct ttcgccatgc gcgctttcac ccaatcacgc
     6181 gctttcgcca cttctgccgc cgggtcttcc gccgtttcgc tccacatctc aatcaggtac
     6241 ggcccgcaat agccactctg tttgagcgtt tcgaaacaac gttcgaaatc cactacacct
     6301 tcgccaaacg gcacgttttt gaagacgcca ggtttggtgt ctttcacatg taccgcgacg
     6361 atatgcccga ttccggcctg caactccatc tgcacatcgt tgtcccacgc cgacaggttg
     6421 ccgatatccg ggtagagctg gaaccacggg ttgttgagat agtgcgcgta acccagcgcc
     6481 ttgctgatgg agttcatcaa cggataatcc atgatctcca tcgccagcgt cacctgcgcg
     6541 cggctcgcca tctcaacgct ctctttcagg ccgtcacgga aacgacgacg cgtttcgtta
     6601 ttggcttcct gatagtaaac gtcatagccc gccagctgga tcacgcgaat gccaacgtcc
     6661 tgggcgaact ggatggcttt acgcataatc tccagcccct gcgcccgcac cgcgtcatct
     6721 tcgctgccca acgggaaacg acgatgggca gaaaggcaca tggacggcac acgcacgccg
     6781 gtttcaacaa tcgcattcac cagcgccaga cgctgctcgc ggctccagtc gaggcgcgag
     6841 aggcgatcgt ccgtttcatc caccgacatc tcgacaaaat cgaagcccaa cgtttttgcc
     6901 agtcgcaggc gttccaacca gcactccccg gcggggagcg ctttttcata gatgccaagc
     6961 gggatttgtt tggacaacat atccgctcct tagccccaca gttcggcgat ggaacgtttg
     7021 aactgacgtg cagcttcaac cggagaagcg gcatcacgga tgctgcgacc agcgataaac
     7081 acatgaatcg gaataccttt gaacagcggc agatcttcca gcgccaggcc accggtgacg
     7141 gtgactttga agcccatatc ggaaagacgt ttgatcgcgg taatgtccgc ttcgccccac
     7201 gctacgcctg cggcctgcgc gtcacggctg cggtgataaa ccacctgctg aatgcctgcg
     7261 tcacgccact gctgcgcctg ttcccaggtc cagtagccgg tcaattcgat ctgcacgtcg
     7321 ccgttaaact cttttgctac gtccagcgcg cctttggcgg tgttgatatc cgcacagcaa
     7381 attaccgtca cccagtcagc gttggcttcg aagcacatac gcgaaaggat tttgcccgca
     7441 tcagcaattt tagcgtctgc cagtacgatt ttgtgcgggt agagcgcttt caggtcacga
     7501 accgcacgca cgccttcgcc cacgcacaga atggtaccca cttcgataat gtcgacttct
     7561 tcggcaatca ggcgagtggt ttcgtaggcg ctatccatag tctggttgtc cagcgcgact
     7621 tgcaacatcg gtaatgacat cttcaattcc ttcttaagct gccgcgttgg tgcggtcaat
     7681 gagatccagt acttcctgct cggtacggca ggcacgtaaa cggtcgaaat tttcttcatc
     7741 ttcaaacagg ttgacgatct gcatgatgcc cacttcctgg tgcgtgttgg catcgaccgc
     7801 cgccatggtg atgaggatat ccaccgggtc gttatcttcg tggttgaact ccagcggctt
     7861 tttcagcgtc accagcgcaa aaccggtttt cttaacgccc tcttccgggc gaccgtgcgg
     7921 cattgccagg cctggtgcga gcacaaagta cggaccgtgc tgttcaaccg catccagaat
     7981 cgcctggtag taacgcggct cgacaacatc cgccgccacc agcaggtcaa cgccgatttt
     8041 tactgcgtcc tgccatgtct cagcttcagc ctgcaggcgg atggatttat tctccgccag
     8101 cgaatcacgt aatttcatgg cgcgtcctta cttcacatcc tgcgggaaat gcgctttgat
     8161 cacttccagc agtttcgggc caaagtcggc aggagagagc atgttgcgca cgccaaccac
     8221 gtatttgttg ccggtcacgg tgatttcgcc cgcaatgtgc gtagaagcga tgatgatatc
     8281 cgcgccgctc aactcgcttt tgtattcgcc aaccgcgcag ctatttaccg tgtggtcaat
     8341 gtttgattgg gttaaaaact ggtccacttt catcttcatg atcatggaac tgccttgtcc
     8401 gttgccacac acagccagaa tacgtacggt cataatcaaa actccttatt aagcagactg
     8461 ttctgccagt tgtttttctg catcttcttc tgcgcgcagt gcgcggccag cgaagaacat
     8521 ataagccagt gcaattacaa tgatgacggc cataaaggcg ataccgatgg agaagaagcc
     8581 ctgcatcatc ggcggtgcca gaatcgacca gtccgccatc cccatccagg cactcatacc
     8641 ggtaagtttc accgcccaga cgcaaccaaa gatttcgatc atccccatca ccagacaaat
     8701 cttcagcgcc gcacgccagc cgccgaagtg gttagcgaac acgccgatgg tggcgttgga
     8761 gaagaacatc gggataaagc ctgggataat caggatcgag gaaccgcagg cgaccaggat
     8821 gccaaccgca atcagctgac cgatggtgcc ccacataaag ccccagacca cggcgttcgg
     8881 cgcgaagcta tagatagccg cacagtcaat cgccagaacc gcacctggga tcaggcgctg
     8941 ggaaatgccg ttaaatgctt cagagagttc cgccacaaac atgcgcacac cctgagtgat
     9001 gatgaagatc gccaccgcga aggagaaacc agtttgcagg atgtacaccg tccagttcac
     9061 tttacctgcc atcgcctgca cggtgtcgat accgaaggag agcagaatgg caccaaagaa
     9121 gatggtcatc acaatcgccg tggagacgat gttgtcgtgg aaaatattca gccagcctgg
     9181 cagtttgagg tcttcaacgc tttcctcttt tttgccgagg aacggcgcaa ctttataggc
     9241 aatccatgat gcaaactgct gctggtgacc aatggagaaa ccgcagccgt cggtgacttc
     9301 ctgagtcggt ttgtacatca tgttggaggt aatgccccag tagagcgaaa ccagaatcgc
     9361 cgtacagata atggtcgtcc acatggaata gccgaaaata aacaacgtaa cggcaatcag
     9421 ccccgcctgc tggaacatga tgtggccggt cagcatgatg gtgcgaatac cggtaatacg
     9481 acgcagcagc acgtaacaaa tgttcagcgc cagtgccagc agcaccgcgt accctaccca
     9541 gctataggca tcgcccatgc ggtcgatggt tgccatcatc gacgcgtaag tatcggaaat
     9601 tgcgccgtta atgccgtaga cttcggacat tttcgccacc accggtttga aggtgctggt
     9661 gaggatgccg gaccctgcct gcaacaacat gaaaccaatt atggttttaa tcgtgccttt
     9721 aataataacg ctgacacttt tgcgcagtag gatgtagccc agacaggtca caatacccag
     9781 caacaacggg gcattggtca tgacctggtt aaaaaacacg gtaaagatgt tgtagaggat
     9841 ctccataacg atctcctgaa gaagaggtaa ccgggtattc cacacacccg gaatgttgtg
     9901 cactcactct aattttcaaa agtaatcaca acaagattat ttatgattaa atgtgacgcg
     9961 acccgcaaat aattccacat ggatagaatg ggattcacac aacgaaacaa ctattcattt
    10021 aaattcattt agttaattga attacacatt ccattaaatc tttccacact caaaatcaac
    10081 aaccacttcc ctggcaaaaa gtcaaaaaat ggtcattgcc ttcctgaaaa aatagtgtca
    10141 ggattaatca aaaccaatca catattgatt cgaattgaac tatcatgaag gtaaatggcg
    10201 atgagtaaag tgaaaagtat cacccgagaa tcctggatcc tgagcacttt cccggagtgg
    10261 ggtagctggc tgaatgaaga aattgaacaa gagcaggtcg cccctggcac atttgcgatg
    10321 tggtggctag gctgcaccgg gatctggttg aaatcagaag gtggcaccaa cgtttgcgtt
    10381 gatttctggt gcggcactgg caaacaaagt cacggtaacc cgttaatgaa acagggtcac
    10441 cagatgcagc gcatggctgg cgtgaaaaaa ctgcagccaa acctacgcac caccccgttt
    10501 gttcttgatc cgtttgcgat tcgccagatc gacgcggtac tggcgactca cgatcacaac
    10561 gatcatatcg acgttaacgt cgctgctgcc gtgatgcaga actgtgctga tgacgtacca
    10621 ttcattggac cgaaaacctg cgttgatttg tggattggct ggggcgtacc gaaagagcgt
    10681 tgcatcgtgg tcaaaccggg cgatgttgta aaagtgaaag acattgaaat tcatgcgctt
    10741 gacgctttcg accgtactgc actgatcacc ctgcctgccg atcaaaaagc ggctggcgta
    10801 ctgccggacg gcatggacga tcgcgcggtg aactatctgt tcaaaacgcc tggcggctcc
    10861 ctgtatcaca gcggcgactc ccactactct aactattatg cgaagcacgg taacgaacat
    10921 cagatcgatg tggcgttagg atcgtatggt gagaacccgc gcggtattac cgacaaaatg
    10981 accagcgccg atatgctgcg tatgggtgaa gccctgaatg cgaaagtggt tatcccgttc
    11041 caccacgaca tctggtcaaa cttccaggcc gatccgcaag agatccgcgt gctgtgggag
    11101 atgaaaaaag atcgtctgaa gtatggcttc aagccgttta tctggcaggt tggcggcaaa
    11161 tttacctggc cgctggataa agacaacttc gagtaccact atccgcgcgg tttcgatgat
    11221 tgcttcacta ttgaaccgga tctgccgttc aagtcattcc tgtaatctgt cgtttatacc
    11281 ggatagcgtg gagaagttcg cgctatccgg cgtagtattt cctggctatt tcaaatatca
    11341 tctaaaaaaa tcaaatttta tcggaatagc tcatgactga agcacaaaga catcaaatcc
    11401 tcctggaaat gctcgcacaa ttgggctttg tgaccgttga gaaagtcgtt gagcgtctgg
    11461 gaatttcgcc tgccactgcg cgacgcgata tcaataaact tgacgaaagc ggcaaactga
    11521 aaaaggtgcg caatggcgca gaagctatta cccaacagcg cccgcgctgg acgccgatga
    11581 atctgcatca ggcgcagaat cacgatgaaa aagtacgtat tgctaaagcg gcctcgcaac
    11641 tggttaatcc gggcgaaagc gtagtcatca actgcggctc caccgcgttt ctgcttgggc
    11701 gggaaatgtg tggcaagcca gtgcaaatca tcactaatta tctaccgctg gcaaattacc
    11761 tgatcgatca agaacatgac agcgtgatca ttatgggtgg acagtacaac aaaagtcagt
    11821 ccatcacttt aagcccgcag ggcagcgaaa acagtctcta tgccgggcac tggatgttta
    11881 ccagcggaaa agggctgacc gcagaagggt tgtataaaac cgatatgctg acagcaatgg
    11941 cagagcagaa gatgctgagc gtggtaggga aactggtggt actggttgat agcagtaaga
    12001 ttggcgaacg cgcgggaatg ctttttagcc gtgccgatca aatcgatatg cttatcaccg
    12061 gcaaaaatgc taacccggaa atcctgcaac aactggaagc gcaaggggtc agcattctgc
    12121 gagtttaaag atgctggcgg aaaaatgtca cggcagcatc taacgcctca ggcgtaatgc
    12181 ggtgacgcac gcctggctgc catgaacagg ttagcagttt atcccgtccc gtctcgctta
    12241 aggcctgctg caaacgtagt gattcgtcgg caggcacaac gtcatcgtcg aggccatgcc
    12301 acagcagtag aggtctgtcc ccaagttgtt ccaggtggtt tgtcgcttcc cactctgcca
    12361 gcggcgcgac aatgttattg aattcattct gctgtgctgc cgtttcaggt atcagcggtg
    12421 gaaacagtga acgggcgagc gatgtaaaat agcccgatcc catcatgctg gcggtacatt
    12481 tcaccgtggg gtggcgagcg gtaattccca gtgccgtcat cgcgcccatc gacgcgccac
    12541 cgactgccag acggtcatca agcagccagt tttcttcggc tattgccgca cgtaaagtag
    12601 tgaattcctg catactttgt agcaagattt gccagaattg atttaaccgc cgcgcttcgt
    12661 cgccactaaa acggctaccg tgatcgggcg catccggcat gatcacccgc aaaccagcct
    12721 gcgccagcgc aacggcaaaa tagctataca ctaggcagga tgaagtaaag ccgtgataaa
    12781 aaattacgca cggtaacggg gtatcttttt gcccgacagg ataagcatga agaacgggaa
    12841 tatctgccag ctcgcgtgat tctatttcaa tcatctgtct ctcctttttg tctggtatga
    12901 catgaagaaa tccttagcac aagtttctgc tggcggtatt agttcataat gttgagatgt
    12961 gggttacgct ttcgtcacgt tttcattcga aaatcgcgta ccaggtaaca attcgggaac
    13021 attcccccaa agttaaatta ataaggcact acactatggt tagcaggaaa cgtaatagcg
    13081 ttatttaccg gtttgccagt ttattattgg tgttgatgtt aagtgcctgt agcgctctgc
    13141 aaggtacgcc acagccagca ccgccagtaa cgaaccatcc gcaagagatt cgccgcgacc
    13201 agacgcaagg gttacagcga ataggtagcg taagcacgat ggttcggggt tctccggatg
    13261 acgcattagc agaaatcaga gcaaaagctg tcgctgcaaa agctgattat tacgttgtcg
    13321 tcatggtgga cgaaaccatc gtgacaggac agtggtattc acaagccatt ttgtaccgta
    13381 aataaatcag acaacttaca tagtcagctt tacattgctt tgcgtcccta tgcgtttacc
    13441 tgtgcacaat aaattaccgg ctggaagcct gggcgtttat taccgcgatg gaatgccctg
    13501 caacatgtgg ggaaacgaaa aatggagctg acgatgaaac aattacttgc ctcaccctcg
    13561 ctgcaattag tgacttatcc tgcgagcgcc acggcacagt ctgcagaatt cgccagtgct
    13621 gactgcgtga cgggcctgaa tgaaataggt cagatctcag ttagcaatat ttcaggggat
    13681 ccgcaggacg tggaacgtat cgtggcgtta aaggccgatg aacaaggtgc gtcatggtac
    13741 cgcattatta ccatgtacga agaccagcag cctgataact ggcgcgtaca ggcgatactg
    13801 tatgcatgag tgttatatac agcaaatgaa caatgccgga tgcggcgcga gcgccttgtc
    13861 cggcctacaa ttttcgcata tttcattgag ttaatcgaaa ccagcgtcgc atcagtcgta
    13921 tacgcttaca cacacactcc ccccgtcgcc cgcagcaata aatcattctg gatctgctct
    13981 gacaaccgta cgccgccgcg cgtatctaac atcacctgac accacgcctg cgccattggc
    14041 ggagaggcat atttcaacat ttgcgcaccg cagcccagca ggaatagctg atgagtaatc
    14101 tctcgcccca gttcttcagc tggcttgcgc agctgctgct gtaaacgacg aaccgcgcga
    14161 tcaaaatagc gatcctgccc tttcacttcc acaaacgctt ccgacaataa gtcgtatacg
    14221 cccgcttgct tattgagaac gcgcagcaca tccaggcaca taatattgcc ggaaccttcc
    14281 caaatactgt ttaccggcat ctcccggtaa agccgcggca gctcgctctc ctcgcaataa
    14341 ccaatgccgc ccagcacctc catcgcttcg gccacaaacg gcatacctct tttgcagatc
    14401 acaaattttg ccgcaggcgt aaataaacgc gcccacaggg cttctttggc atcggcacgc
    14461 cggtcccacg ctcgcgcaag acgaaacagc aacgccgttt gcccttcaag ctgaagtgcc
    14521 atgcgactta agacatgacg cataaggggc tgttggatca atggattacc aaaaacatgg
    14581 cgttgatgtg catgataaat cgccagcgaa aatgcacggc gcatcatggc atggctaccc
    14641 agggcgcaat caaaacgcgt catcccaccc attttcagga tcagacgaat tccttcccct
    14701 tcctgcccca acaaccagcc aatggcatcc tgaaactcca cttcgcagct ggcgttagag
    14761 cgattaccca gcttatcttt cagccgctcg aggcgaatcg cgttgcgttg cccgtcaggc
    14821 aaaaagcgcg gcacaaaaaa gcaggacaaa ccgcccgtag tctgcgccag caccagatgc
    14881 gcatcgcttt gcggcaccga gaaaaaccat ttatgcccca ccagccgata agagccatct
    14941 tccaggcgct ctgcgcgggt ggtgttgctc atgacatcgg aaccgccctg cttttccgtc
    15001 attcccatgc caatcaacaa accgcgtttt tgcccacctg gcaataagtg agaatcgtag
    15061 cgatcgctca gcagcggcgt ggtccagtcc tgaaacggcg tgggtaacat ctgtaacaac
    15121 aatggcgtgg cggcaaaggt catcgttatt ggacataacg acccagcctc aacctgcgca
    15181 tgtaacataa aacgcgccgc gcgcgccaca aatgcgccgg agcgagcgtc ttcttcccag
    15241 gcaagattgt gcacccgatt ggtacataac gcctgcatca gcaggtgcca ggcggggtga
    15301 aaacgcacat cgtccagacg gcgtccttgc gcatcgtagc gcaataattc aggcggattc
    15361 acattcgcca gccgccccag ttcaagagat tcagccgttc ctaactgctg accaatactg
    15421 gctagaaaat cgctatccca gccagcacct tcacgcgtta ccgcttcaca gagcgcgcca
    15481 tcagacaggt ataagttgct gttatttaat ggtataggtt gattaaaaac ggtgtgagtt
    15541 tgccagtgca ctgtgtctcc ctccatcaat ggcagtcacc cttagtatgg tcactgccat
    15601 ggattcctga cagaaagaga ttgctaaaac attcttatcc agcaatgtaa tgcggaacga
    15661 aacgtgaggt gtctttggtg atcagcgtgt tatcttcacg gatccccatt ccgcacgctt
    15721 catcatcgac aatccagcta ccgatgagtg tgtagctatc gccaaatcgc ggcagaggtt
    15781 gaaacgcctg gtagatcatc ggttcatcgg cgtaatcacc atcagcgtgg tcaacgacgt
    15841 tattctgacc gtcgaaaatg gtgacgtttc cgccttcgcg cgagtagatc ggtttacgca
    15901 catagctttc gccagcggca atctgcggtt tttcgccctc aaaccaggat gcaagaaggt
    15961 taggatgacc agggaagaag cgccaaagca gcggcattag ccctttatta ctcaagatac
    16021 ttttccataa cggctccacc cattgctcgc gacgcttgcg cagcagcgga ccgttatcgt
    16081 cacgcatcat ccactccagc ggatacagct taaatgcacg ctggatgaca ttatcatcaa
    16141 gatcggtcag tacaccgccg acgcccaaac cgagatcttc aatgtagata aaccgcgact
    16201 cctgccctgc ctgctgggcg cagtcctgca agtacagcac ggtactcctg tcttcatcgg
    16261 tgtcctgaca gcagcaaaaa taaaacggtt cccgactgta aagctcgcta aagcgtgaaa
    16321 tcaggcgttc ctgaatagca ttgtactgat cggcatcacg cggaataatg ccgctgcgcc
    16381 gggcatcctc cagccacagc cactggaaat aagccgactc gtacaatgaa gttggcgtat
    16441 cggcgttgta ctccagcagc ttcaccggcg cattgccaca ccaggcaaaa tccatccgtc
    16501 catacagcga aggatcgcgg gcgcgccagc tttcagcgat cacatcccag tacaacggcg
    16561 ggatcgccag ttgcgtcagg atctcttcgt ctttaaccgc tcgatccacc acctcaaggc
    16621 acatctgatg cagttccgca gtcggttttt cgatctgctc ttcaatctgg cgcagagtaa
    16681 aacggtaagc ccgactctca tcccaataga tttcattgtc gatgatatga aagtcgaaac
    16741 cgttgtcagc ggcgatctgg tccagatccc gccgcacagg aacgttgtgt cttagcatga
    16801 ttagcctccc caatgcccac gggcgctgga agaacgacca tagccgccgc gagaaacggt
    16861 tgacgctttt ttggtggtaa agccgcccga agagtaagac tcttttttgc cagaaccgga
    16921 gcgccaggag taatcaccgg aagtgttgcg ccagacgggg cgggaagcaa atgaggatcc
    16981 gccgctgtta taaacaaact gctcatcgcg atctttacga ataacccggc ttaacaaaaa
    17041 tccagatatt accgggatcc aactctgttc aacgttgtcg tagtaacaat tttcatactt
    17101 agactgacag ttctgctgag tcatattctt cggaacatcg gcataaaacg ccgctttggc
    17161 gttgttccag ccacgcgcgc aaatgtcggc attattaccg tcatcaatac aatcctgcac
    17221 tgtcgaataa aacgttccgt cgccgtcgtt atcgacatcg ccactatcgc cacaaccttt
    17281 caatacaaaa aaagcggcac cgcccattag agccaacgtt aagtattttt gtgcgtagcg
    17341 attgcgacgc ggttcaaaag gattattcgg tctaccaatg cgactgatcg ctccgtgacc
    17401 aattttactg ttatttcttg atttgcgttt tctggccatg atcgtccatt accatgtcat
    17461 acaagctgcg ttaaaaatac cgccagccag cgcggcggtt cccataaaca ttcctgctgc
    17521 ggtattgtga ttaataattt tttcgcttaa tgcgggcata taaagtctga caccagcaaa
    17581 aacgagtaac tgaatcacca gtgcaatccc gccccaggcg aaatagtctg gaatactcac
    17641 cgcattgatt gccgcactgg ataatgggat aacgtaacct aacaatgtac cactgaacgc
    17701 cagtgaagcc gcggtattat tgtttttgat taactgccat tcgttgtgcg gtgtaatttt
    17761 agaatagata aaaagaaaaa taatcaccat agccacgcca ataaaaaaat aggcgctaaa
    17821 ggcaagaagt gaatccagta tatgcattgt aaacgtcctt gtaattaacc aataatatga
    17881 agtgatgtca gcgggatatc gacacccaac gcgcgcgaaa ataaccactc tggctcgccg
    17941 agatcgttaa aagattcctc accatttaac agcaaatatt cgtaagtatc ttcggttact
    18001 tgccgctggt agcccatggt aaaattatgg acctcccatt tggcatggtt ttgattttct
    18061 actttttcca gcatgtagac gggttcgata tttcccggtt cttcgctgtt aaaaaatcgc
    18121 tgccagcgtt tttcctgcca gtttaagatc attgccccca tcactttggc gttgatcgcc
    18181 tcgcgccagt gactctcttt actgataccg tagctctctt catagacaaa gagcttgata
    18241 tcatcaatgt catcaatatc ttcgccgccg gtggtattga tttgcagaaa ttcatcgcct
    18301 gaagtgtagt agcggaaaat ctgactaccg ccgcccagat cgatgtggct gacagcggct
    18361 accgtaaatt cctcacccgg cagcgcaatc agcaattcat cttccagcaa acgaaacgct
    18421 aacgtgtcga gcgtaaagcc actattgaga tgaagtccca gcggaccgcg agcgatagca
    18481 ggcttattat ccttgccaaa cagacgctgg aaaaaaccag acatatttta tccttaaaat
    18541 agttgccagc cttttagcga ggctggcaaa aaaattactc gccctgttgg cgttgcagtc
    18601 ttgctaatac atcctgggcg ctacttttat tgctaccgcc aattccggct tccgccagct
    18661 tttcatcaag atcgcgaccg tctgcgactt tttccaactg tgcagcagca tccaggcgag
    18721 cctgacgttc ggcctgacgc gtttgcaggc gttttaagga ttctgccgct gtcgaaacgc
    18781 tggaagatgc gccaacggta gaggttgtta cagcctgttg tgcacgctgc atggcttcag
    18841 tggctttaac gacttccatt tgctgctcaa actgagcgat acgctgcgct gtcgctgtaa
    18901 ctgctttttc cacgccatca cgggagactt ccagattcga caacacttgc tcttcagcgg
    18961 taatgagatt ctcaaggcgc gcgatttctt cagcaacttc gttaatcaac gacggattaa
    19021 cgttcttgct taacgcttcc agcgcacggg cttccagact ggctttgcgc tcacgtaaat
    19081 ctttcagctt atcgtgactt aatttcaccc gcgccagcag atcaacgcga gattttccgg
    19141 ctttatcgag ttcagcttta gcgtcacgaa tatgctgttc cagcatgcgc acgccctggg
    19201 tttcttcaat ggattcttcc gcctgggaga taaacgattt ccccagcgta aataaacttt
    19261 ttaaaattcc cataaatacc cgtccttgta attagtgtga atattcttct gtaatttcag
    19321 ccagatccaa tgcgttatct accagcgagg ttatctcaag caggatatct tcaagagaag
    19381 atttaagcga taacgccccg aaaacaatgt aatactcttc ctgttgcacg ctggagattc
    19441 ctaccgatga caacggcatc atcttctgat ttcttaataa gaaggtatta aattcatctg
    19501 gattgctgat actacttacc ggacaaataa aggtttcgat aatcatttgg cgggaagtaa
    19561 aaagaatatt gatttgcaaa tcgccataat cattcatttt aataattaat gcgctctcgc
    19621 tatttgtcac atcaatattt tgttcaggta cagtttgcag cgccgtcgcc agcgccaacg
    19681 gattccatgt catatattat tccatataga tttagtttaa atattaataa aatgaatatt
    19741 tgcagtacgt aattatctta ccagctatag acaaaaaaaa accatccaaa tctggatggc
    19801 ttttcataat tcagacgaac tagctgcgct gacgaaccgc ttcaaataag caaattccgg
    19861 ttgcaaccga aacgttcagg gaagaaacac ttcctgccat tgggatgctg atcaactcat
    19921 cgcaatgttc acgggtcagg cgacgcatac cttcaccttc cgcgcccatc accagcgcca
    19981 ggcgtccggt cattttgctc tgatagagtg tatgatccgc ctcgcctgcc gtaccgacga
    20041 tccagatatt ctcttcctgc aacatacgca tggtgcgcgc aaggttagtc acccgaatca
    20101 gtggaacgct ttctgccgcg ccgcaggcta cttttttcgc cgtagcgttg agctgtgcgg
    20161 agcgatcttt cggcacaatc accgcgtgaa cgccagcagc gtccgcgcta cgcaggcacg
    20221 cgccgaggtt gtgcggatcg gttacaccgt cgaggatcag caggaacggt tgatcgagcg
    20281 aagcgatcag atccggcaga tcgttttcct gatactgacg tcctggcttc acgcgggcga
    20341 taatgccctg atgcacggca ccgtcgcttt tctcgtcgag atattggcgg tttgccaact
    20401 ggataaccac gccctgagac tcaagggcgt gaatcagcgg taacagacgt ttatcttcac
    20461 ggccttttaa aataaagact tcctgaaaac gttcaggggc gcgctccagc agggcctgca
    20521 ctgcgtggat gccgtaaatc atttcgctca ttaatgtact cgttgtttac gttttgcctg
    20581 atgcactacg tttatccggc ctacatgatc tctgcaatat attgaatttg cgtgattatg
    20641 taggccggat aaggcgttca cgccgcatcc ggcatgaaca aagcgcattt tgtcagcaat
    20701 ctaaccctct tcttttaaag agggtattga tcactctgcc acttttttct tcgccgcacg
    20761 cttcgctttg gtcgctgcgg ctattttctg cgttttcgcc gatggctttt tcgcttttct
    20821 cgcgtctttc ttcgccgctt tcggcttcgt ttttttctca ccgcggaagg cgctgtctgg
    20881 ctcaaagttt acctttttac cgacctgacg acgcttgccg ccttttttgc ctgcatcgcc
    20941 ttttttcgct ttctcgcgcg ccgttttacc gacgttgcgc ggtgcgcgtt cactggagat
    21001 cagactaaag tcgattttgc gctcgtccat attaaccgct tcgacgcgaa cttccacgcg
    21061 atcgcccagg cgataagtct ggccgctgga ttcccccatc aggcgttgcc ctacctggtc
    21121 aaagcgatag tagtcattgt ccagcgaaga gacatggacc agaccatcaa tgaacaagtc
    21181 gtccagacgg acgaagaagc caaagccagt gacgctggaa attacgcctt taaagacgtt
    21241 acctacctgg tcgagcatga agtcacactt cagccagtca gcgacatcgc gcgttgcttc
    21301 gtcggcacga cgttccgcca tcgaacagtg ctgacccagt tgcaacatct cttccatcga
    21361 ataatggtag ccgccggttt cagtggtgtt gccctgatgc ccctgctctt tcgacagcag
    21421 atatttaatg gcgcggtgca gcgtcaggtc aggataacga cgaatcggcg aagtaaagtg
    21481 cgcataggac tgcaatgcca gaccgaagtg accacggttt tctggatcgt aaatcgcctg
    21541 tttcatcgag cgtagcagca tggtttgcag catttctgca tcaggacggt cggcaaccga
    21601 ctccagcagt tccgcgtagt cacgcggttc cggcttgtta ccacccggca gctccagccc
    21661 cagctccgcc agcactgaac ggaaagaggt aatcgcttcg gtgctcggct tgtcgtgaat
    21721 acggaacagt gccggttctt tcgctttctc aacgaaacgc gccgccgaga tattcgccag
    21781 aatcatgcac tcttcaatta atttgtgcgc gtcgttacgc tgggtctgtt cgatacgttc
    21841 aatacggcgt tcagcgttga aaatgaactt cgcttcttcg ctctcaaatg agatcccacc
    21901 gcgttcttca cgggctttat ccagcacttt atagaggtta tgcaactctt cgagatgctt
    21961 aaccagcggg gcgtactgct cgcgcagatc ctgatcgccc tgcagaatat gccagacttt
    22021 ggtgtaggtc agacgcgcgt gagagctcat caccgcttcg tagaatttgt agcccgtcag
    22081 gcggcctttc gacgaaaccg tcatctcgca caccatacac aggcggtcta cctgcgggtt
    22141 gagcgaacac aggccgttag agagcacttc cggcagcatc gggataacct gcgaagggaa
    22201 gtacaccgac gtgccacggt tacgcgcttc tctgtccagc ggcgttggcg gacgcacata
    22261 gtagctgacg tcggcaatcg cgacccataa acgccagccg ccgccgcgtt ttttctcgca
    22321 gtaaactgca tcgtcaaagt cacgggcgtc ttcgccatca atggtgacca gcggtaaatc
    22381 gcgcaaatcg acacggcccg cttttgcttc ttccggcact tcttctttca gcccggcaac
    22441 ctgttgctca acagcctgcg gccagatgta cggaatttca tgggtacgca gagcgatatc
    22501 aaccgccatg ccggtgccca tattatcgcc aaggacttcg acgattttac ccaccgcttt
    22561 ggtgcggcga gtcggacgct gagtcagttc gactacgacc acaaagccca tccgcgcgcc
    22621 catgatctga tcgggcggga ttaagatatc gaagctcaga cggctatcgt caggaaccac
    22681 aaagccgacg cccgcttcgg taaagtagcg accaacaatc tggctggttt ttggcaccag
    22741 tacgcggaca atacgcgctt cacgacgacc tttacggtca gcgcccagcg gctgagccag
    22801 cacctgatcg ccatgaatgc aggttttcat ctgctcgctg gagagataca aatcatcttt
    22861 acgcccttca acccgcagaa agccgtagcc atcacggtgg ccaataacgg tacctttcac
    22921 caggtcgagg cgttccggca gcgcatagca ctgacgacga gtgaagacca gttgaccatc
    22981 gcgctccatc gcgcgcaggc ggcgacgcag gccttcaagc tgctcttcgc cttcaatgtg
    23041 cagttctacc gccagctcat cacggctggc cggtttttca cgtttggtta aatgttcgag
    23101 gataaattcc cgactaggga tgggattcgc gtatttttca gcttcgcgtt cctggaaagg
    23161 atcttgtgac atctcggttc ctccgttgtc atctctgatg aagattttcg tcactccacc
    23221 agcaataatt tataaagcgg ttgattctct tcaaccaaat cggcaagcgt gtagttatcc
    23281 agttccgtaa gaaaactttg cacggcctta gaaagcgcct gtttcaaccg acaggcaggt
    23341 gtaatgtggc aaaactcact gctgcaattc accagcgata agggctccag ctcgcgcacc
    23401 acatcaccaa tacgtatcgc actcgccggt ttacccaggc gaatgccgcc attttttcca
    23461 cgaacagcag tcacgtagcc ggcacgacta agttgattga ttattttgac catatgatta
    23521 cgggagacgc cgtagacgtc agtcacttca gaaatactgg tcatccgccc ttctggcaat
    23581 gacgccatgt agatcagcgc acgtaatccg taatcagtga aactcgttaa ctgcacatca
    23641 acctcaaaag ggaaatcggg aaaaaataca tttatattga tgataaacca gccacaagct
    23701 gtgtcgctaa tttatttcag ttcggggaag gaaaaaagcg aggatttaac actgtgtcgg
    23761 atagcgtaat ggcttaaccg acacagcaat gtatcacact gttagcccgg caggcaaaat
    23821 atctgccagg cgtaccagaa ttacgcgtcg aacggatcgc gcagaatcat ggtttcagta
    23881 cgatccggac cggtagagat gatatcgatc ggcacaccag tcagctcttc aatacgcttg
    23941 atgtagttca gcgccgcctg cggcagaccg ctacgatctt tcacgccgaa ggtggattca
    24001 gaccagcccg gcatggtttc gtaaatcggc tctacacctt tccagtcgtc agctgccagc
    24061 ggagtggtcg tcacttcacg accatccggc atacggtaag ccacacagag tttcacctct
    24121 ttcaggccat ccagaacgtc cagtttagtc aggcagaagc cagacaggga gttcagctgt
    24181 accgcacgac gaacggcaac ggtgtccagc cagcctgtac gacgacgacg ccccgtagtt
    24241 gcgccgaatt cgttaccctg cttgcagagg aactcgccag tttcatcaaa cagttcggtc
    24301 gggaacggac ctgcacctac acgagttgag taagctttga ggatacccag aacataatca
    24361 acataacgcg ggcccaggcc ggaaccggtc gccacgccac cagcagtggt gttggaagaa
    24421 gttacgtacg gataagtacc gtggtcgata tccagcagcg taccctgcgc accttcaaac
    24481 atgacgaaat cgccacgctg acgcgcctgg tcgagcaggt cagaaacgtc aaccaccata
    24541 gaagtcagga tgtcggcaac agccatcgta tcatccagaa ctttctggta atcaaccgct
    24601 tcagctttgt agtagttaac caactggaag ttgtgatatt ccatcacttc tttcagtttt
    24661 tcagcgaagg tttctttgtc gaaaaggtcg ccaacacgca gaccgcgacg tgctacttta
    24721 tcttcataag caggcccgat accacgaccg gtggtgccga tcgctttcgc gccacgcgct
    24781 ttctcacgcg cgttatccag cgcaacgtga taatcaagga tcagcggaca tgcttcagac
    24841 agcagcagac gctcacgaac ggggatgcca cggtcttcca gttctttcat ctctttcatc
    24901 agcgcggccg gagacagcac aacaccgtta ccgatgatgc tggttacatt ctcgcggaga
    24961 atacctgatg gaataagatg gagaacggtt ttttcaccgt tgattacgag agtatggcct
    25021 gcgttgtgac cgccctggta gcgtacaaca tatttagccc gttcagtcag aagatcgacg
    25081 atcttacctt taccttcgtc accccattgg gtgcccagta cgacgacgtt gttacccatt
    25141 tttcaaaatc accgtttgct taaaaatgga ttctaccatc gctttttcag agttacagca
    25201 ctttttgcac tcaaaaatga cctcgatcag tctgtttttt gttcagccaa tcgttttcct
    25261 caacatgtag tagaccacaa cgcccgcaac cacaagtcca ccgccaaaac gacgtaaaat
    25321 attatcgggc aaattggtca tcgcagagat catcttcttc catgccttcg ggtaaagcat
    25381 cggccctaaa ccttccagta ccaaaaccag ggcaagcgcc agccagattg tcgaattcat
    25441 tcattatcct tatagaaaaa gaaaaccacc gacatccctg aggatgcggt ggctttattg
    25501 acctgtaccg cagtcgttat attaacgcgt tgcggaagtc ggcgtcttca tgtagcggaa
    25561 gaaatcgcta tccgggctca tgaccatcac gtcctgattg ccagagaagc tgttctcata
    25621 agcacgcagg ctacggatga atgcgtagaa gtccggatct ttactgaatg catcagcaaa
    25681 cagtttggct gcttcggcat cgccttcacc acgcatgatg cggccctgac gctcagcttc
    25741 tgccagcgtt ctggtcactt catagtcggc agtcgcgcgc agtttttccg cttcttcctg
    25801 accttgtgaa cggtgacgac gcgctaccgc ttcacgctcg gcgcgcatac ggttgtagat
    25861 cgcttcagac acttcggtcg gcaggttgat ctgcttaatt cgcacatcga caacctcaat
    25921 acccagcgcc gccatactgt tcgggttgat gaccggaact ttgcccttcg tctcagccgt
    25981 tacgcgctct gccgcttcgg caatggcgtt atctgccgcc ggggtagtaa cttcatcttc
    26041 tgtacccgca gaaccggagt tcagcgcgtc acgtacttcg agggtcagac gaccacggga
    26101 atcggtgacg atatctttca cgtccaggcg accaatttca gaacgcagac ggtcagagaa
    26161 cttacgtttc aacagcactt ccgcttgcga aatgtcgcca ccacccgttg ccaggtagta
    26221 acggctgaaa tcgctgatgc gccatttgat gtaagagtcg acgatcaggt ctttcttctc
    26281 tttggtcaca aagcggtcgg cctggttgtc catggtctga atacgtgcgt cgagcatttt
    26341 caccgtttca atgaacggta tcttgaaatg cagacccggc tcataaacca gaggtttgtt
    26401 gtcatcgtca cgcagtacct taccaaaacg cagcgtaata ccgcgctcac cttctttgac
    26461 gacaaagaca gacatgtaaa gcactaccag cacgatgatg ataatcgcga taactgactt
    26521 acgcatcgtt attccccctg acgctggtag tcgttacgct gcgcgttggc gcggcgttgg
    26581 tccataatat cgccctgact ggtggacgac gtgttgcttg ctccactggt tgtggaggaa
    26641 gaggctggcg gcagacgcag cagattgctg gtaccgttat cgctcttcgc cgcaggggcg
    26701 ttaccgcctt tcagcatctg gtctaacggc agaaccatca ggttgccacc tttatcgtta
    26761 accagcactt tgcgggtgtt acccaacact ttttccatcg tctcgatata cagacgctcg
    26821 cgagtaattt ccggcgcggc tttatattcc ggcagaagtt tagcaaagcg cgccacttca
    26881 ccctgagctt ccaggatggt ctgggccttg tacgcacgcg cctcttcgag gatacgttgc
    26941 gcctgaccgt tcgcacgcgg ctgaacttcg ttggtatacg cttctgcttc acgaatgtat
    27001 tgctgttcgt tttcacgcgc ggcaatcgca tcgtcaaacg ccgcttttac ttcttccggc
    27061 ggacgagcag cctggaagtt gacgtccagc agcgtgatac ccatgtcata cggacggata
    27121 gtctcttcca gttcgcgctg agtatcgcta cgaatcacgg tacgaccttc cgtcagaatg
    27181 cggtccatgg tgtatttacc gataaccccg cgcagggcgc tgtcggtagc ctgacgcagg
    27241 ctgtcatccg ggctggtcac gctatacaga tatttttccg gattggtgac gcggtactgc
    27301 acgttcatct caacgcgcac tacgttctcg tccgacgtca gcatcacacc agaagcggcc
    27361 agttcacgca cggcttccac gttcaccggt ttgacttcgt cgataaacgt cggtttccag
    27421 ttcagacccg gctcaaccag atggctgaat ttaccaaagc gtgttaccac gccgcgttcg
    27481 gcttctttaa tggtatagaa accactggcc gcccagataa tgacaatcgc tgccgctgcg
    27541 atggtaacga cacgaccgcc aagctgcggg cgcgggcctt gcgatgaact gccaccgcca
    27601 gatccggtgc ctttaccgcc gcccagacca ccgagctttt tgctcagttt gcggaagata
    27661 tcatctaaat caggtggccc ttgatcgcga ccgcctttgt ttccatttcc ctcagagttg
    27721 ccgccaggtt tgctgcttcc ccacgggtcg cggtcttgtc cgttattacc gggctgattc
    27781 cacgccatgt ttgtgctcca tatttgttat gcggtgatcc ctgttgtctt tcgacgccag
    27841 aggatatgac tccacgcttc agacgctacg ccgttagatc aggtaatcga tcaacgccgg
    27901 ttcttgttta cagaggcgac gccagtcaac gatcggcata cgaacttgca gacttacgct
    27961 gccgtcctcc tccatccact ctttttctat tgcctgaagc tgataaaaac gacttctcag
    28021 acgcccttcc tgcggcggca aacgcaatgt atgctgcgcc acctcgccgg aaagccgctc
    28081 cgtcaaagcc tgaaaaagct gtggtatccc cgctccggtc tgtgcggaaa gccagacacg
    28141 gatcggtttg ttctcttcgt cccgatcaat acgcggttcg aaatcttcca gcatatcgat
    28201 cttgttcatc accagcaggg ttgggatctc gtgagcgtcg atctcttcaa gaaccgtatt
    28261 caccgcttcg atgttttctt gtacacgcac atccgccgca tcaatgacgt gcagcagtaa
    28321 tgtggcttgc cgcgtctctt gtaacgtggc tttaaatgcc gccaccagat cgtgcggcag
    28381 gtggcgaata aaccctacgg tatctgcaag tacggtttca ccgacatctg caacgtcaat
    28441 acgccgcaac gtcgggtcga gggtggcaaa caactggtct gccgcgtaga cccgcgcttc
    28501 ggtgatgcga ttgaaaaggg tagatttacc ggcgttggta tatcccacca gcgaaacagt
    28561 aggaacgtcg gctttgatac gcgattgccg cccctgctca cgctgctttt caactctttc
    28621 caggcgtgac tgtatctgca cgatgcgatt acgcaacaaa cgacggtcgg tttcgagctg
    28681 ggtttcaccc ggaccacgca aacctatccc gcctttctgt ctttcaaggt gggtccagcc
    28741 acgcaccagg cgcgtagcca gatggcgcag ctgcgccagc tcaacctgca acttaccctc
    28801 atgggtacgc gcacgttggg cgaaaatatc taaaataagg ccggtgcggt cgataacacg
    28861 acactcgcac aaacgctcca ggttacgctc ttgcgccggg ctcagggcat ggtcaaaaag
    28921 aacgaccgaa gcacccgtcg ctttgacagc ttccgcaatt tcaactgctt taccttcacc
    28981 tacaaaatac tttgggtgcg gcgctttacg gctaccggta atcacctgca atgcttcgac
    29041 accggcggaa gagaccagag attcaaactc ctggaggtct tccatatctt tgtcttgcgt
    29101 aaaatagatg tgtaccagta ccgcctgctc accagcatca taacggtcaa acaagcgtat
    29161 aaccctctaa atagatcagc ggggaacgca ggatcgctgg ctccccgtgt aaaaaaaaca
    29221 gcccgaaacc ttattcggtt tcttcgctgt cctgttgcgc ggaagtattc tgcgcgctgc
    29281 taccatgatg gtagttactg ctggtaccgc caccggcgtt gttactgtga tgagaaaccg
    29341 ggcgagacgg gacaacagta gaaatcgcgt gcttgtaaac catctggctg accgtgtttt
    29401 tcaacaggat cacgaactga tcaaaagact cgatttgccc ttgcagctta ataccattca
    29461 ccaaataaat agaaactgga acacgttccc gacgcagtgc gttcaggaac ggatcttgta
    29521 aagattgccc cttagccatt ctctcttttc cttatatgct tatttgtact ttgaaccttt
    29581 cgattctgaa aaaattgcgc acgatacgtc tcaattgtac acattcagcc tgcgatagca
    29641 ccaacaacct gtaatacttc gtcgcgcgcc tgttctggtt tttcactgtc aagccagtga
    29701 accccttccc aaccacgcag ccaggttatc tgccgcttcg ccaactgtct cgtggcgcaa
    29761 acacctcgat aaaccatttc atcgtatgag atttcgcctt caaggtaaga ccacatctgg
    29821 cgataaccca cgcaacgaat ggaaggcaag tccgtatgca aatctcctcg ggcaaaaagc
    29881 gcccggactt ctgcttcaaa acctgaagcc aacatctgat gaaaacgctg ctcaatgcgt
    29941 tgatggagca gttcacggct cgccggggcg atggcgaact gatgcacctg atacggtaga
    30001 gcgtctcctg acgtttgcgt cagttccgtt aaagttttac ccgaaatgaa aaaaacttcc
    30061 agtgcccggg aaagcctttg tggatcattt ggatgaatcc ttgctgccgc aaccggatct
    30121 acctcctgaa gttgacgatg caatgactcc caaccttgct ctgccgcctg ttgctcaatt
    30181 ctggcccgta cttccgggtc tgccgacggt agcggcgaca acccttccag caatgccttg
    30241 aaatacaaca tcgtaccgcc cactaacagt gggatccgcc ccgccgcggt gatatcggcc
    30301 atttccgcca gcgcatcgcg gcgaaaatca gcagccgagt aagcctgcga cggatcgcga
    30361 atatccagca atcggtgcgg cgcggcgagt aactcttcag cgttcggctt cgccgtcccg
    30421 atatccatcc ctttgtaaat aagggcagaa tcaacgctta tcaactctac tggtaaaatt
    30481 ttacgcagct caatggctaa cgccgtttta ccggaggccg tcggccccat caaaaaaatc
    30541 gccttaggca ggctcgcctt actgatatca ctcatctttc agggctttta tcgccggatg
    30601 taaatcaaca gattgtaaca gaccacccgg cggcgttttc acaagttgcg gacataaccg
    30661 ttccacgtcc gccagcaggg ttatggcctg tgccattgac cactgcgcat gttcgctcat
    30721 cagatttcgt gcaatccact gcgcaatatt gccaggttcg aatacggact gcttcgccag
    30781 gtagcctatc agttcaggaa tcaagatttg taaattttgt tggcgtaagg gtaaaggcac
    30841 ggccctgatg gtcacatgct gtgcatctga ctggaaatca atacccaatt ccgccagggc
    30901 agactgcgct ttttctaatg ccgatttttc ttcgccagaa acttttagcc gcaacggaat
    30961 aagcagcggc tgagcgcaaa ctggcgcttc acccggcgtc agttgtacct gacgcagcca
    31021 acgttctgcc actggcaagg ctaaaagtga aatgttgccg tcgcgctcca gcaacgcaca
    31081 gtcggaatgg acgatagtca gtacccgacc aaaactctga ctgttcgccg caagtgcagg
    31141 ttcctgcggt tccggcgctt ttaatttttg catcggcgcg ggcgtttgca aaagctggcg
    31201 atacacttca ccttgctgtt tctggtagcc tggctgcgca ttcggccagg gggcagccgg
    31261 acgactgcct gatgctggcg caggagtgta gcgcggagct accggctcac gagctgccgg
    31321 ttctgcaaag tgattgcgcc ccgccgccac gcggttttcc ggaatggaac gcggtgcagg
    31381 ttggggttca tcgtccagcg gtagcggcgt ttccagttgc tgttgtagca cgctcagcac
    31441 gccctgatag ataaaatcat gcaccagacg cgactgatgg aaacgcactt cgtgtttggc
    31501 ggggtgcacg ttgacgtcca cctgatgcgg atcgatctcc agatacagca caaatgccgg
    31561 ttgctgatcg gcccccagtt tgtcttcgca ggcctggcgg atcgcgtgat tgatcaggcg
    31621 atcgcgcatc atgcgaccgt tcacgtagca atactgaatt tctgccagtg cgggcgtggt
    31681 gtgatttgga tcggccaccc agccgcgcag ggtgagatcg ccgtgttgcc attcaatcgc
    31741 cagcgcttgt tcaagaaaag cggtgccgca aatcgcgcct aagcgccgtt ctttttgccc
    31801 gccttccggc actgcgcggt actgacgcac aattttaccg ttatgcgaca ggttgatcgt
    31861 gacgtcgaaa cgcgccagcg caatgcggcg gatgatctca tcaatgtggt taaattcggt
    31921 tttctcggtg cgcaggaatt tgcgccgcgc cggggtgttg tagaacagat ccagcacctc
    31981 cagcgtcgtc cccacaggat gcgccgccgg ttttaccgtc acgttcatat cgcgcccttc
    32041 ggcataggcc tgccaggctt cctgctgttc tgcggtgcgt gaagtgagcg tcaggcggga
    32101 aaccgaactg atactcgcca gcgcctcacc gcgaaagccc aggctgataa tggcttcgag
    32161 atcgtccaga gaggcgattt tactggtggc atgacgagcc agcgccagcg ccagctcatc
    32221 ttttttgata ccgcagccgt tatcacgaat gcggataagt ttcgccccac cgcgttcgat
    32281 atcaatatcg atacgcgtcg cgcctgcatc gaggctgttt tccaccagtt ctttgactac
    32341 cgacgcaggt cgctcgacca cctcacctgc ggcaatctgg ttcgccagtt gtggcggtaa
    32401 gacctgaatt ggcatcaatc gtccttagtt tggcagcgtg cgatctggcg tcgtcaccgt
    32461 actggcagtt tgtgccgttg ccccctgcgg cgcagattgc atcggatgcg caaggaaata
    32521 attgcgcagg cctttgtaaa tggcttctgc cagctgttgt tggtaatcgt cgctcgccag
    32581 caaacgttct tcgctgttgt tgctgataaa accggtttcg accagtactg acgggatatc
    32641 cggcgaacgc agaacgccga gactcgcgtg ttctggtcgg cgtttatgta tttcgccaat
    32701 gcgttgcaac tgactgatca tactggtcgc tacatcatac cctacccgtt gggaatgacc
    32761 gaactgtaaa tccagcaccg cctggcttaa ataggggtca gactgactgt tcgccagcac
    32821 atcacccgcc ccacccagca gctccgactg tttctcgtgc tgctccagcc agctggccat
    32881 ttcgctgttg gcgcgacggt tagagagcac ccatacggaa gcgccagtcg cactgcggtt
    32941 cggtgcggca tcagcgtgaa tcgacacgag gaaattggcg ttttgcttac gtgccacatc
    33001 actgcgcccc atcaccgaga taaagtaatc cccgtcacgg gttaaaacgc ccttaaacat
    33061 cggatcgtca ttgagcaaag tacgcaattt acgcgcgatg gcgatggtga catttttctc
    33121 ccgcgtacca ccggggccga tagcgccagg gtcctgaccg ccgtgtccgg catcaatagc
    33181 gatgataatt ttatcgccag tgttagccgt cgcgcgcgct gccggacgcg ttaccgtatt
    33241 actgctgata acacccgtag tgcggttact ttccgtttta aacggattgc gcgccggttc
    33301 gctgacgcgc ggtgcgacaa ccgcaggcgt ttcaacgcgt ttcgcaacca cgggcggcgg
    33361 aggaggaggt ggcggcgcat cggcgttaat cgtaaagacg acagtgtaat tgctgccatt
    33421 ctgccgcttc accgcttcgg ttttaccgtt ttcggtaaga tcgaccacca gccgtagcgt
    33481 ttgtgcatct ttaggcgttc cagagcgaat cgccttcacc agattattgc cgctgaacaa
    33541 caacggcagt ccctgaatca cgcccgtttg tttgatatcg agcgccacgg tgcgtttgct
    33601 ttgatggcta aacgcataat caggatcgcc aataaaactc aacgttatcc gcgcctgttg
    33661 attaccgtta gaaacctgaa tatcagagag cgtcgcggca cccaccggcg tgcacagcag
    33721 cagcagcgtc gctaccaacc aatttctgat gcgatacatc atcccgccac ctttcaaagg
    33781 ttaaccggct aaacgcgcca gcaacaattc acccgcagag gaaaccgcac tcacgcgcgc
    33841 ctcacggcct tgtgcctgat aatcaatgtg tatttcgaca tccgggtcag gaagaacacc
    33901 agtaccttgt tgtggccact ccaccaggca gatggcatcg ttggcaaaat aatcgcgaat
    33961 ccccataaac tccagctcct cgggatcggc aaggcggtac aaatcaaagt gataaaccat
    34021 taaattgtcg agcgtatagg gttcgaccag cgtataagtg gggcttttga cattaccctg
    34081 atgacccaga gcttgtaaaa agccccggct aaaggtggtt ttacctgcgc ctaaatcgcc
    34141 atacagatag attacggttg cgccatcgca ggctttcgct acccgctcgc ccaggtctaa
    34201 tgttgcctgc tcatcaggga gcggaattac tcgattcatc atggttttta tcagtcactt
    34261 ccgggttaac aatacgctgt agcgtggaaa agagatcggt tgccagcatc ccgcgcgttc
    34321 caaaacgcgc cgccagtacg tcagctgcag caccgtgcgc gacacagccc gcacaggcgg
    34381 catcatacgg cgacagtttt tgcccaagca atgcgccaat aataccagag agcacatcgc
    34441 ccatgccgcc gctcgccatg cctgcatttc cggcatcaat aatgcctaaa gcgtcaggat
    34501 gggcggcgac cacggttccg gcacctttca gcaccgctac gccgccataa cgttgtacca
    34561 gacgtttggc gcaatgtaag cggtcacttt caatttcagc gacggaacag cctaacaacc
    34621 gtgcggcctc gccaggatgc ggcgtgatca cgcgattgtg acgcttatcg ggattgattg
    34681 ccagcaggtt caatgcatcg gcatcccaca acatcggttt gcgaaaattc tcaacttttt
    34741 gcagtgcttt tttcccccac tcttgctggc ccagaccggg accaatcacc accacatcgg
    34801 cccattccag gctttcggta agagagtcca tcgtcagttc atgcaccatc aattccggtc
    34861 gtgcagtcag cagcggcgca atgttttcac tgcgggtcag tactcggact aaaccagcac
    34921 cagcacgtag cgccgcttcc cccgtcatac gaatagcccc cgccgtgccg tgatcgccac
    34981 cgataattac cagccgcccg tgatcgcctt tatgcgaagt cgggcgacgc ggttttagcc
    35041 agtgagaaag ttgttctgcc gaaaaccgct gaattttcgt ctcctgacct gccagccaac
    35101 tatccagccc cagtgagtca aaatgcagtt gtccggtaac atcccgcgct tttccagtga
    35161 gcaagcctgg tttcagcgca ataaaagtga tggtgtgatc ggcgttgatc accgcgcctg
    35221 gcgtagcgcc agtttcagcc agcaggccgg aagggatatc aaccgccaca atcggcgcag
    35281 gatgggaatt agcgtggtcg attaactggc taatggattc gcggggcgct tgccgcaaac
    35341 cggtaccgag cagcgcatca acaatcagat ctaccgattc gggccagaca atattcgaag
    35401 catggatctc gccacccgcg tttaaccatg cttcgcgtgc cagcgcggcc tcttccggca
    35461 acggtttgtc gctctcctgg gccaacaacg tgacctcaat gccgaccgct ttggccagtc
    35521 gcgcgaccac gtagccatcg ccgccgttat taccatgacc gcacagcacc agccagtggc
    35581 gggcgtcagg atacgccgaa cgacacacct ggaatgcggc ctcgccagcg cgaagcatca
    35641 gctcatagag tgtgagcccc agcacatctg ccgcctcgcg ttctccgcgg cggatatcgt
    35701 cggcgtacca gacggtgtgt ggtatactta cggggttttt cttcattgta tggtccgtca
    35761 tgtcagagcc cctcgatctc aatcagttag cgcaaaaaat taaacagtgg ggactggaac
    35821 tgggctttca gcaggtaggt attaccgata ccgatctcag cgagtccgag cccaaactgc
    35881 aagcatggct ggacaaacaa taccacggcg aaatggactg gatggcacgt cacggtatgc
    35941 tgcgcgctcg cccccatgag ttattgcccg gtacgctgcg cgtgatcagc gtgcggatga
    36001 attaccttcc tgctaacgcc gcatttgcca gcacgctgaa aaaccccaaa ctcggctatg
    36061 ttagccgtta tgcgctgggc cgtgactatc acaaacttct gcgcaaccga ctcaaaaagc
    36121 tgggcgagat gattcagcaa cattgtgttt cgctgaattt tagaccgttt gtcgattctg
    36181 cgcctattct cgagcgcccg ttagctgaaa aagctgggct cggctggaca ggtaagcact
    36241 cacttatcct caatcgcgag gccggttctt tcttcttttt aggcgaattg ctggtcgata
    36301 ttccgctgcc cgtggatcaa ccagtcgagg aaggatgcgg taaatgcgtg gcctgtatga
    36361 cgatttgccc gaccggtgcc atcgtcgagc catataccgt cgatgctcgc cgctgtatct
    36421 cttatctcac catcgaactt gaaggggcga tcccggaaga gttgcgaccg ttaatgggaa
    36481 accgtattta cggttgcgat gactgccagc ttatctgccc gtggaatcgc tattcgcaac
    36541 tcactacaga agacgatttc agcccgcgta agccgctaca cgcaccggaa ctcattgagt
    36601 tattcgcctg gagcgaagag aagtttttaa aagtcacgga aggatcggcg attcgtcgta
    36661 ttggtcacct gcgttggctg cgtaatatcg ccgtagcatt aggcaatgcc ccttgggatg
    36721 aaacgatttt gacagcgctg gaaagtcgta aaggtgagca cccacttctc gatgagcaca
    36781 tagcgtgggc gattgcgcag caaatagaga gacgaaatgc gtgcatagtc gaagtgcaat
    36841 tgccgaaaaa acagcgtctg gttcgggtga ttgaaaaagg gttaccgcgt gacgcctgat
    36901 tcatccacag cttgtgaata aaaataaaaa cgcattgcaa ttcaagacga ataaaatcgt
    36961 caagtgatcg actcgacaaa acggaatctt aatttttaac ttatctatca ataggttaaa
    37021 aaaatactat tcaccgtgcg aagtttcttt gaaattaaga ttacagcctg aggctgtgga
    37081 taactctgtt tacaagagtt tgtcagagac aacgaaaacc agcaataacg catcgcatcg
    37141 ctgtggatat tttattgaga gaagaatttg gagcgggaaa cgagactcga actcgcgacc
    37201 ccgaccttgg caaggtcgtg ctctaccaac tgagctattc ccgcttgggt ggtctgtgcc
    37261 ttacagcact ttcaaatttt ggagcgggaa acgagactcg aactcgcgac cccgaccttg
    37321 gcaaggtcgt gctctaccaa ctgagctatt cccgcttggg tggtctgtgc tttgcagcac
    37381 ttttcaaatt ttggagcggg aaacgagact cgaactcgcg accccgacct tggcaaggtc
    37441 gtgctctacc aactgagcta ttcccgcgta ctacttaatt ttgctttcgt aattttcgca
    37501 tttctgcgtc gttacgggag gcgcattata cgagaaatct ttccctctgc aaccccccaa
    37561 aaaagttttt tttgcgtttt tatctcaagt gctgaaatta tcagcaagac gttcaattta
    37621 gcgtcaaaat cacgccgccg caagtggatt ccggcgctta cgtgaccagg aaaaatttta
    37681 cagcttgata aaatgctcgc ggtagtaagc cagttccgcc accgattcac ggatatcatc
    37741 catcgcctga tgcgtcccct gcttggtaaa accatccaga atttccggct tccagcggcg
    37801 cgccagctct ttcagggtgc tgacatcgag atagcggtag tggaagtagg cttccagctc
    37861 cggcatgtat ttaaacagga aacgacggtc ctgtccgatg ctgttaccgc aaatcggcga
    37921 ttttcccgca ggcacccact gttttaagaa ttcgagcgtt gccagttcag cttcccgatc
    37981 gcccatcgtg ctcgctttca cgcgctctac cagcccgctg gcggtatggg tgcgcacgtt
    38041 ccagtcatcc atcagcgcca gctgttcatc ggactgatgt actgcaatgg tcggcccttc
    38101 tgccagaata ttcaggttgg catcggtcac cagcgtggca atctcaataa tgcgatcgcg
    38161 ctcgggatcc agaccggtca tctcaagatc gatccaaatc aggttgtttt cattggcact
    38221 catgctattt tccacccatt cacagtgact atattgatcc acaattgcgt gtatcataga
    38281 tgttttgccc atcaggggcg accaggagtc tgtacgattg agtaaaaata aactctccaa
    38341 aggccagcag cgccgcgtga acgccaatca ccagcgtcgt cttaaaacgt ctaaggagaa
    38401 gcccgactac gacgacaatc tgtttggcga gcctgatgaa ggtatcgtca tcagccgctt
    38461 tggtatgcac gctgatgtgg aatccgccga tggcgacgtt caccgctgca atattcgccg
    38521 taccatccgt tcgctggtga ccggcgaccg cgtagtctgg cgtccgggta aaccggcggc
    38581 ggaaggcgta aatgtcaaag ggatcgtgga agcggtgcat gagcgcacct cagtgttgac
    38641 gcgtccggat ttctacgacg gcgtgaaacc tattgccgcc aacatcgacc agattgtcat
    38701 tgtctccgcc attttgccgg agctgtcgct caatattatc gaccgttacc tggtggcctg
    38761 cgaaaccttg cagattgagc cgattattgt gctcaataag atagacctgc tggacgacga
    38821 aggcatggcg ttcgtcaacg agcagatgga tatctaccgc aacattggtt atcgcgtatt
    38881 gatggtttcc agccatactc aggatgggct aaaaccgctg gaagaggcgt tgaccgggcg
    38941 catcagcatt tttgccggcc agtctggcgt cggcaaatcc agcctgctga acgcgttgct
    39001 ggggctgcaa aaagagatcc tgaccaacga tgtctctgac aactcgggtc tcggtcagca
    39061 caccacgacc gccgcacgac tgtatcactt cccgcacggc ggtgatgtga ttgattcccc
    39121 tggtgtgcgt gagttcggcc tctggcacct ggagccggaa caaatcactc agggctttgt
    39181 cgaattccat gactatttag gtctgtgtaa atatcgcgat tgcaaacacg ataccgatcc
    39241 gggctgcgct atccgggaag cggttgagga aggaaaaatc gcggaaaccc gtttcgaaaa
    39301 ctatcaccgt attctggaaa gcatggcgca ggtaaaaacg cgtaaaaact tttctgatac
    39361 ggatgactga caattaagct aaccgtcgtt agaatcgtcc cctttttttc aggatccggc
    39421 atgtaggccg gatcaggaac gacaaaacaa tggcctggag gctaccttgt taaattcatt
    39481 taaactttcg ctacagtaca ttctgccgaa actatggctt actcgcctgg cgggttgggg
    39541 cgcaagcaag cgggcaggat ggctgacaaa actggttatc gatctgttcg ttaaatacta
    39601 caaggtcgac atgaaagagg cgcaaaagcc ggacaccgcc agctaccgca cctttaacga
    39661 attctttgtc cgtccgctgc gtgacgaagt acgcccaatc gataccgatc cgaatgtcct
    39721 ggtcatgcct gccgatggcg ttatcagcca gttgggtaaa atcgaagaag ataaaatcct
    39781 gcaagccaaa ggccacaact acagcctcga agccttgctg gcaggcaact atctgatggc
    39841 ggacctgttc cgcaacggtg cgtttgtgac cacttacctc tccccgcgtg actaccaccg
    39901 cgtacacatg ccgtgcaacg gtattctgcg tgagatgatc tacgtgccgg gcgatctctt
    39961 ctccgttaac catctcaccg ctcagaacgt gccgaatctg tttgcccgta acgaacgcgt
    40021 gatttgcctt ttcgataccg aatttggccc aatggcgcag attctggtcg gagcgacgat
    40081 tgttggcagc attgagacgg tctgggcggg caccattacg ccgccgcgcg aaggtatcat
    40141 caagcgctgg acctggcctg ccggggaaaa cgacggttct gtggcactgc ttaaaggcca
    40201 ggaaatgggt cgctttaaac tcggttccac cgttatcaac ctgtttgcac cgggtaaagt
    40261 gaatctggtt gagcaactgg aaagcctgtc tgtcacgaaa attggccagc cgctggcagt
    40321 atctaccgaa acctttgtta cgccagacgc tgaacctgcc ccgcttcctg ctgaagagat
    40381 cgaagcagaa cacgacgcca gcccattggt tgacgacaaa aaagaccagg tctaatccat
    40441 caaaggaaac gctgacgtgc gcctgattat cacttttctg atggcctggt gcctcagttg
    40501 gggggcgtac gccgcgacgg cccccgatag caaacaaatc acccaggaac tggagcaggc
    40561 aaaagcggcg aaacccgcgc agccagaagt catagaggca ctccagtctg ccttaaatgc
    40621 gcttgaggaa cgaaaaggtt cccttgagcg catcaaacaa tatcagcaag ttatcgataa
    40681 ttacccgaaa ctctccgcta ctctgcgcgc acaattaaac aacatgcgtg acgagccgcg
    40741 cagcgtgtcg ccgggaatgt ctaccgatgc gctgaatcag gaaattctcc aggtcagcag
    40801 tcagttgctg gataagagcc gtcaggccca gcaagagcag gagcgcgccc gcgagattgc
    40861 cgattcactg aatcaactgc cgcaacagca aaccgatgcc cgccgtcagt taaatgagat
    40921 cgagcgccgc ctgggaacgc ttaccggcaa tactccgctc aatcaggcac aaaatttcgc
    40981 gttgcagtct gactccgcac gcctgaaagc gctcgttgat gaactggaac tggcgcagct
    41041 gtctgccaat aaccgtcagg aattagcgcg cttgcgctca gagctggcgg aaaaagagag
    41101 ccagcaactg gatgcgtatt tgcaggcctt acgtaatcag ttgaatagcc aacgtcagct
    41161 tgaggcggag cgggcgctgg aaagtaccga attactggca gaaaacagtg ccgatttgcc
    41221 gaaagatatc gtcgcgcaat tcaaaattaa ccgcgaacta tcggcggcac tgaatcaaca
    41281 ggcgcagcgg atggatctcg ttgcctcgca acagcgtcag gctgccagcc agacgttaca
    41341 ggtccggcag gcgttgaata cgctgcgtga acagtcgcaa tggctgggat cgtccaacct
    41401 gctcggtgaa gcgctgcggg cgcaggtggc acggctacca gaaatgccaa aaccacaaca
    41461 gcttgatacc gaaatggcgc agttgcgtgt gcaacggtta cgttatgagg atctgcttaa
    41521 taaacagccg ctgctacggc aaatccatca ggccgacggt cagccgctga ccgccgagca
    41581 aaaccgtatt ctggaagcac aactgcgcac tcagcgtgag ttgctgaact cattgttgca
    41641 gggtggcgac acgctactac tggaactgac caagctgaaa gtctccaacg ggcaactgga
    41701 ggatgcgctg aaagaggtga acgaagcaac gcaccgctat ctgttctgga cctctgacgt
    41761 gcgcccgatg accatcgcct ggccgctgga aatcgcccag gatctgcgtc gtctcatttc
    41821 gctggacacc ttcagtcagt tgggcaaagc cagtgtgatg atgctgacca gcaaagagac
    41881 gattttgccg ctgtttggcg cgttgattct ggtcggttgc agtatttact cgcgccgcta
    41941 tttcacccgt tttcttgaac gttcggcggc gaaagtcggc aaagtgactc aggatcactt
    42001 ctggctgacg ttacgcactc ttttctggtc gattctcgtc gcgtcaccgt tgccggtgct
    42061 gtggatgacg ctgggttacg gcttgcgcga ggcgtggcct tatccgctgg cggtcgcgat
    42121 tggcgatggc gtaacagcca ccgtgccgct gctgtgggta gtgatgattt gcgctacttt
    42181 tgcccgcccg aacggcttgt ttatcgctca ttttggctgg ccgcgcgaac gggtttcccg
    42241 tgggatgcgc tactacctga tgagcatcgg gcttattgtg ccactgatta tggcgctgat
    42301 gatgttcgtt aacctcgacg accgcgagtt ttccggttcg ctgggacggc tttgctttat
    42361 cctcatttgc ggtgcgctgg cggtggtcac cctcagcctg aaaaaggccg ggatcccgct
    42421 gtatctcaac aaagaaggca gcggcgacaa cattaccaac catatgctgt ggaacatgat
    42481 gattggcgcg ccactggtcg ccattctggc gtcggcggtg ggttatctgg caacggcgca
    42541 ggcactgtta gcgaggcttg aaacctcggt tgccatctgg ttcctgctac tggtggttta
    42601 tcacgttatc cgccgctgga tgctgatcca gcgccgcagg ctggcgtttg accgggcgaa
    42661 gcatcgccgg gcagagatgt tagcgcaacg tgcgcgtggc gaagaggaag cgcatcatca
    42721 cagtagcccg gaaggagcaa ttgaagtcga tgaaagcgaa gtcgatctcg atgccatcag
    42781 tgcgcaatcc ttgcggctgg tgcgctcaat tttgatgttg atcgccctgc tttctgtcat
    42841 tgtgctgtgg tcagaaatcc attccgcttt cggcttcctc gaaaatattt cgctgtggga
    42901 tgtcacctcc acggtacagg gcgtagaaag tctggagcca attaccctcg gtgcggtgct
    42961 gattgccatt ctggtgttta tcatcaccac gcagctggtg cgcaacctgc ccgcgctgct
    43021 ggaactggcg attttgcagc acctggattt aacgccaggt acgggttacg ccatcaccac
    43081 catcaccaaa tatctgctga tgctgattgg cgggctggtc ggcttctcaa tgattggtat
    43141 tgagtggtcg aaattgcagt ggctggttgc cgcgctcggt gttggtctcg gttttggttt
    43201 gcaggaaatt ttcgccaact ttatctctgg cctgatcatc ctgttcgaaa aaccgattcg
    43261 cattggcgat acggtaacga tccgcgatct tactggtagc gtgacaaaaa ttaacacccg
    43321 cgccaccacc atcagcgact gggaccgtaa agagataatc gtgccgaaca aggcgtttat
    43381 taccgagcag tttatcaact ggtcgctctc tgactcggtc acgcgcgtgg tgttgacgat
    43441 tccggcccct gccgatgcca atagcgaaga agtgacggaa atcctgctca ccgcagcgcg
    43501 tcgctgctcg ctggtgatcg acaacccggc accggaagtc ttcctggtgg atctgcaaca
    43561 ggggattcag attttcgagc tgcgtattta cgccgctgag atgggtcacc gtatgccgct
    43621 acgccatgag atccaccagc taattctggc aggcttccat gcccacggta tcgatatgcc
    43681 attcccgccc ttccagatgc gtctggaaag cctcaacggt aagcaaacgg ggagaacatt
    43741 aacttctgcg ggcaaaggtc gtcaggcggg aagtttgtaa tgcatgaagc tgccaaatga
    43801 tgtcaacgtg gctcatttag cagcttaaca tcatcttcag ttatcaccgg ccctggagag
    43861 tatcatccat acgcattact tcctgacaac tcatcgccgg agagacgatg agttgcagga
    43921 tttcatatga tcagtcgaga gctaactcag gtttattcgc catgcgactg caatagttct
    43981 ggtaaatcgc catcgctaac agcgagaaga agacagggcc gccaatcatc cacaatgtgc
    44041 tgtcccagtc tccggcttca accacaggtt gaatgatggt gaagacgttc gcaaatgtca
    44101 ccaccagtac gacaaccact gtcgcaatca ttgccgacaa atgcgttttg aaaatcacaa
    44161 acggtctgtc gagatcctga cgtgctttaa agaacgggaa agccagcgcg aggaacaggt
    44221 aaggaagcgt catagacacg ttcgccatca gcgtcagctt gttaaagaac gccgatgcgg
    44281 taccgccacc aaacgaaacc agcaggatga agacagtaac caacccgcac tgcatccaca
    44341 tggcgataga aggcatcccc atcgcattca ggcgcgtcat cggttccggc cacaacgctt
    44401 tcggcgtccc ctggatgatg gctttcagcg gtgaatagca aagcgtaaag aacgcaccgg
    44461 tataagcgag gaacatcgaa agtccggtaa tacgcgcaaa ccatacaccc agcgacaatg
    44521 acgcttcagg tgacaaatgc agtgcgttac ccagcgtcac cccgaggctc ttcatcagca
    44581 cataggtaat attgccgagg ttaacggaac cattacttaa tacctgctgc cagtttgtgc
    44641 tgacacccca taaaaatatt gccagcgaat aaccgattga aataacaata gcggcaaaaa
    44701 caataccttt ggcaaagttc ttttctggat tttccgtttt atcgaccaga ccaccaaccg
    44761 cttcaattcc gccataggca aaaatagcaa ataccacaaa cgatagcatt gccagaccgg
    44821 actgataacc tgggttcggt gatgcaagga aattaatatc ctgcgcgaaa tgcccaccat
    44881 ttaataacaa aatagtaatg cttataacag caatactaaa ttcagacaca ttactgcaat
    44941 accgcccacc gcagtaatgc gggcaatttt attaatcccc tttgaagcaa cgacggtgac
    45001 cagaatcatc catgctactg ccagtagacc aaccacctgc gtaggctcca gtccggcaat
    45061 acgccagtgc tgggtcatgt cgctaccata gaggaatgtt gagaacggta cccaaacttt
    45121 cgcggaggta ctcaccatcc agatgatata agaggaaaac cacataaacg taccaatgaa
    45181 ggcaaaacgt ggtccgacac tattattcat ccaggaatag ataccgcctt cttctttgcg
    45241 ataagcagct cccatttcag ccatcattaa ggcgaatgga ataaagaata acaatgcaga
    45301 aaatatataa aagggaatcg cactataacc cattaagtaa taagccgatg ggctattggc
    45361 aaatccaaat acggaagtaa agatcatcaa tatgagtcct atcagactca tcttttttat
    45421 cgtgtgaggc atgaaaccat ccttcatttc agcgcgtgta taacacgtgc ccactgaact
    45481 gcactgcgca acattgagca ggcagcgtaa aaaccaatac agaattgata cctggatgat
    45541 atcaaatatc cagtcttaaa ttgtggcctt taacggaaaa atgaaaacat atgctacaga
    45601 atggcgctta tcacgccatt cttcgctgtt aattcagtaa tttttcagaa ttatgcccgg
    45661 tcaacgctaa aggcgatgac ttcagccagt gtctccgcgc ccagcgccaa catcaccaga
    45721 cgatcaacac ctaatgccac gccggaacag tcaggcatac cgactttcaa ggcttcaatc
    45781 agattctggt caatggggtg ctgcggcaaa ccgcgcgccg cgcgcttacg gttatcttgt
    45841 tcaaagcgtt gttgctgctc acgggcatcc gtcaattcat ggaaaccatt cgccagctca
    45901 atacctttat aataaacctc aaagcgttca gcgacccgat gatcttcggt actgatttgc
    45961 gccagtgatg cctggctggc tggaaagtgg tacacaaagg tcggtttttc tttgccaata
    46021 tttggctcta cgccaaaggt aaacagcaat tgtagcagcg tgtcgcggtc ttcttcggta
    46081 tcagcaacat tgctcaaatc cagtttcgct gcgacttccc gcagttgcgt tttgtcggca
    46141 gagagcgggt caatttccag ataacgcaag aaagcttgtt gataagaaag gctttctgct
    46201 gccgggcagt ccagcacctg ttgtaagaga tcgtccacct cgttcatcaa ccggtacata
    46261 tcatagtgcg gtcgatacca ctccagcata gtgaactcag ggttgtgata acgccccatc
    46321 tcttcattac ggaagctgcg gcacagctgg aataccggcc cacaaccggc aaccagcagg
    46381 cgtttcatat ggtattccgg gctggtcatt aaccagagat tcatcccctg cgaatgcccg
    46441 gggccaacga aacgtgtctc aaacgggacc aaatgaatat cggttaccgt cgcctggctc
    46501 atacaaggcg tctccacctc cagcactcca cgatcggcaa agaaacgacg gatctccgcc
    46561 ataatcgccg cgcgttttaa taagttagga atggatgcgc tcggctgcca ggatgccgtt
    46621 tcgctcatag ttaaatctcc agtttttgac aagggcacga agtctactcg caacgcgacg
    46681 gcgagacaaa ttttacgcag gaatcaaaca gcggtgggca gtgactaaaa aaagcacgat
    46741 ctgatggttt agtaattaaa ttaatcatct tcagtgataa tttagccctc ttgcgcacta
    46801 aaaaaatcga tctcgtcaaa tttcagactt atccatcaga ctatactgtt gtacctataa
    46861 aggagcagtg gaatagcgtt cgcagaccgt aactttcagg cacttaccct gaagtacggg
    46921 gctgtgggat aaaaacaatc tggaggaatg tcgtgcaaac ctttcaagcc gatcttgcca
    46981 ttgtaggcgc cggtggcgcg ggattacgtg ctgcaattgc tgccgcgcag gcaaatccaa
    47041 atgcaaaaat cgcactaatc tcaaaagtat acccgatgcg tagccatacc gttgctgcag
    47101 aagggggctc cgccgctgtc gcgcaggatc atgacagctt cgaatatcac tttcacgata
    47161 cagtagcggg tggcgactgg ttgtgtgagc aggatgtcgt ggattatttc gtccaccact
    47221 gcccaaccga aatgacccaa ctggaactgt gggggtgccc atggagccgt cgcccggatg
    47281 gtagcgtcaa cgtacgtcgc ttcggcggca tgaaaatcga gcgcacctgg ttcgccgccg
    47341 ataagaccgg cttccatatg ctgcacacgc tgttccagac ctctctgcaa ttcccgcaga
    47401 tccagcgttt tgacgaacat ttcgtgctgg atattctggt tgatgatggt catgttcgcg
    47461 gcctggtagc aatgaacatg atggaaggca cgctggtgca gatccgtgct aacgcggtcg
    47521 ttatggctac tggcggtgcg ggtcgcgttt atcgttacaa caccaacggc ggcatcgtta
    47581 ccggtgacgg tatgggtatg gcgctaagcc acggcgttcc gctgcgtgac atggaattcg
    47641 ttcagtatca cccaaccggt ctgccaggtt ccggtatcct gatgaccgaa ggctgccgcg
    47701 gtgaaggcgg tattctggtc aacaaaaatg gctaccgtta tctgcaagat tacggcatgg
    47761 gcccggaaac tccgctgggc gagccgaaaa acaaatatat ggaactgggt ccacgcgaca
    47821 aagtttctca ggccttctgg cacgaatggc gtaaaggcaa caccatctcc acgccgcgtg
    47881 gcgatgtggt ttatctcgac ctgcgtcacc tcggcgagaa aaaactgcat gaacgtctgc
    47941 cgttcatctg cgaactggcg aaagcgtacg ttggcgtcga tccggttaaa gaaccgattc
    48001 cggtacgtcc gaccgcacac tacaccatgg gcggtatcga aaccgatcag aactgtgaaa
    48061 cccgcattaa aggtctgttc gccgtgggtg aatgttcctc tgttggtctg cacggtgcaa
    48121 accgtctggg ttctaactcc ctggcggaac tggtggtctt cggccgtctg gccggtgaac
    48181 aagcgacaga gcgtgcagca actgccggta atggcaacga agcggcaatt gaagcgcagg
    48241 cagctggcgt tgaacaacgt ctgaaagatc tggttaacca ggatggcggc gaaaactggg
    48301 cgaagatccg cgacgaaatg ggcctggcaa tggaagaagg ttgcggtatc taccgtacgc
    48361 cggaactgat gcagaaaacc atcgacaagc tggcagagct gcaggaacgc ttcaagcgcg
    48421 tgcgcatcac cgacacctcc agcgtgttca acaccgacct gctctacacc attgaactgg
    48481 gccacggtct gaacgttgct gaatgtatgg cgcactccgc aatggcacgt aaagagtccc
    48541 gcggcgcaca ccagcgtctg gacgaaggtt gcaccgagcg tgacgacgtc aacttcctca
    48601 aacacaccct cgccttccgc gatgctgatg gcacgactcg cctggagtac agcgacgtga
    48661 agattactac gctgccgcca gctaaacgcg tttacggtgg cgaagcggat gcagccgata
    48721 aggcggaagc agccaataag aaggagaagg cgaatggctg agatgaaaaa cctgaaaatt
    48781 gaggtggtgc gctataaccc ggaagtcgat accgcaccgc atagcgcatt ctatgaagtg
    48841 ccttatgacg caactacctc attactggat gcgctgggct acatcaaaga caacctggca
    48901 ccggacctga gttaccgctg gtcctgccgt atggcgattt gtggttcctg cggcatgatg
    48961 gttaacaacg tgccaaaact ggcatgtaaa accttcctgc gtgattacac cgacggtatg
    49021 aaggttgaag cgttagctaa cttcccgatt gaacgcgatc tggtggtcga tatgacccac
    49081 ttcatcgaaa gtctggaagc gatcaaaccg tacatcatcg gcaactcccg caccgcggat
    49141 cagggtacta acatccagac cccggcgcag atggcaaagt atcaccagtt ctccggttgc
    49201 atcaactgtg gtctgtgcta cgccgcgtgc ccgcagtttg gcctgaaccc agagttcatc
    49261 ggtccggctg ccattacgct ggcgcatcgt tataacgaag atagccgcga ccacggtaag
    49321 aaggagcgta tggcgcagtt gaacagccag aacggcgtat ggagctgtac tttcgtgggc
    49381 tattgctccg aagtctgccc gaaacacgtc gatccggctg cggccattca gcagggcaaa
    49441 gtagaaagtt cgaaagactt tcttatcgcg accctgaaac cacgctaagg agtgcaacat
    49501 gacgactaaa cgtaaaccgt atgtacggcc aatgacgtcc acctggtgga aaaaattgcc
    49561 gttttatcgc ttttacatgc tgcgcgaagg cacggcggtt ccggctgtgt ggttcagcat
    49621 tgaactgatt ttcgggctgt ttgccctgaa aaatggcccg gaagcctggg cgggattcgt
    49681 cgacttttta caaaacccgg ttatcgtgat cattaacctg atcactctgg cggcagctct
    49741 gctgcacacc aaaacctggt ttgaactggc accgaaagcg gccaatatca ttgtaaaaga
    49801 cgaaaaaatg ggaccagagc caattatcaa aagtctctgg gcggtaactg tggttgccac
    49861 catcgtaatc ctgtttgttg ccctgtactg gtaaggagcc tgagatgatt aatccaaatc
    49921 caaagcgttc tgacgaaccg gtattctggg gcctcttcgg ggccggtggt atgtggagcg
    49981 caattattgc gccggtgatg atcctgctgg tgggtattct gctgccactg gggctgtttc
    50041 cgggtgatgc gctgagctac gagcgcgttc tggcgttcgc gcagagcttc attggtcgcg
    50101 tattcctgtt cctgatgatc gttctgccgc tgtggtgtgg tttacaccgt atgcaccacg
    50161 cgatgcacga tctgaaaatt cacgtgcctg cgggcaaatg ggttttctac ggtctggctg
    50221 ctatcctgac agttgtcacg ctgattggta tcgttacaat ctaacgtatc gccaatgtaa
    50281 atccggcccg cctatggcgg gccgttttgt atggaaacca gaccttatgt tcaaaacgac
    50341 gctctgcgcc ttattaatta ccgcctcttg ctccacattt gctgcccctc aacaaatcaa
    50401 cgatattgtg catcgcacaa ttaccccgct tatagagcaa caaaagatcc ccggtatggc
    50461 ggtggcggta atttatcagg gtaaacctta ttactttacc tggggctatg cggacatcgc
    50521 caaaaagcag cccgtcacac agcaaacgtt gtttgagtta ggttcggtca gcaaaacatt
    50581 tacgggcgtg cttggtggcg acgctattgc tcgaggggaa atcaagttaa gcgatcccac
    50641 aacaaaatac tggcctgaac ttaccgctaa acagtggaat gggatcacac tattacatct
    50701 cgcaacctac actgctggcg gcctgccatt gcaggtgccg gatgaggtga aatcctcaag
    50761 cgacttgctg cgcttctatc aaaactggca gcctgcatgg gcgccaggaa cacaacgtct
    50821 gtatgccaac tccagtatcg gtttgttcgg cgcactggct gtgaagccgt ctggtttgag
    50881 ttttgagcag gcgatgcaaa cacgtgtctt ccagccactc aaactcaacc atacgtggat
    50941 taatgtaccg cccgcagaag aaaagaatta cgcctgggga tatcgcgaag gcaaggcagt
    51001 tcatgtttcg ccaggggcgt tagatgctga aacttatggt gtgaagtcga ccattgaaga
    51061 tatggcctgc tgggtacgaa gcaatatgaa tccccgtgat atcaacgaca aaacacttca
    51121 gcaagggata caactggcac aatctcgcta ctggcaaacc ggcgatatgt atcagggcct
    51181 gggctgggaa atgctggact ggccggtaaa tcctgacagc atcattaacg gcagtggcaa
    51241 taaaattgca ctggcagcac accccgtaaa agcgattacg cccccaactc ctgcagtacg
    51301 cgcatcatgg gtacataaaa caggggcaac cggcggattt ggtagctatg tcgcgtttat
    51361 tccagaaaaa gagctgggta tagtgatgct ggctaacaaa aactatccca atccagcgag
    51421 agtcgccgcc gcctggcaga ttctcaacgc tctacagtaa aatttcatcg ggtccgaatt
    51481 ttcggacctt ttctgcgctt ttccttgctg tcatctacac ttagaaaaaa accagtaagg
    51541 aaacatttat gcgcctgctc cctctcgttg ccgcagcgac agctgcattt ctggtcgttg
    51601 cctgcagttc tcctacgccg ccgcgtggcg taacagtagt gaataatttc gacgccaaac
    51661 gttatctcgg tacctggtat gagattgccc gttttgatca ccgttttgaa cgtggactgg
    51721 aaaaagtcac cgcaacatac agcctgcgtg atgacggcgg cctgaatgtc attaataaag
    51781 gctataaccc tgacagagga atgtggcagc agagtgaagg gaaagcgtac tttaccggcg
    51841 caccaactcg cgctgcgctg aaagtgtcat tctttggtcc tttctatggc ggttataacg
    51901 ttattgcact cgatcgggaa taccgccatg cgctggtttg cggcccggac cgcgactacc
    51961 tgtggatact ctcccgcacg ccaaccattt ctgacgaagt gaaacaggag atgctggcag
    52021 tcgcgacccg ggaagggttt gatgtcagta agtttatttg ggtacagcag cccggtagtt
    52081 agtgagtgct gagtttcaga ccaataatcc ccaatacgat taacgccaga ctcgccaggc
    52141 gcatcgggtt agcggactca ccgagcagca caatgccagt gatagccgcg ccgactgcgc
    52201 caatacccgt ccacacggca taagccgtcc ctactggtaa cgatttcatc gcccaggcaa
    52261 gtagcgccat actgacaatc atcgccgtca cggtaataac actcggcgtc aaacgactaa
    52321 agccgtgggt atatttcagg ccaacggccc ataccacttc cagcagacca gcaataacta
    52381 agataatcca ggacatatca ggctccggag aattggggcc gtccccggtg aaaagaggcg
    52441 tttgcaggtc gtcctgcaaa gctgatggtt gaacggatat tttgccaggt ggcaggggaa
    52501 tttcaactat taccttagtg actatcgcag aaaaaacaaa cggcacgaca tacctgtcgt
    52561 accgttattg cttattgcta ttgctgcgct ttcgttgctg cgccagaaat cgcgttaccg
    52621 ccatcagaaa tgtcttcacc aacgccacgc gtggtgttgc aggcagttaa tactgttgaa
    52681 agcaccagaa cagaaaagat cgctgcaatt gtcttcttca ccataacgtc ttccttttag
    52741 gtaatgttat ttatgtttgc ctatagcaaa ttaataatag acaacatcgc caaaaatgac
    52801 ggaaatctga tgatttttcg gaagagaaga aaaattagct ggcagcgcga gagatggagt
    52861 tgccgagatg tttgatgtct tcgccgaaac cgcgagcggt gttacagccc gtgagcagcg
    52921 tgctggcaag caaaacaaga acgataaggc gtttcatcat ctctgacctg aataagtgat
    52981 ggtgcagcct gcaggccgca ccacaaccgc attacttcac gcgagagacg tattcaccag
    53041 agcgggtatc cactttgatg acttcgccga tttgtacgaa cagcggaacc ttaaccacag
    53101 cgccagtaga cagggtagcc ggtttgccgc cagtacctgc ggtatcacct ttcaggcccg
    53161 gatcggtatc aacgatttcc agttcaacga agttcggcgg agtaacggag atcggctgac
    53221 cattccacag agttacgata cactctgcct gatccagcag ccatttagcg ttgtcgccaa
    53281 ttgcttttgc atcagcagac agctgctcga aagtttcgtt gttcatgaag tgccagaact
    53341 caccgtcgtt gtacaggtaa gtcaggttca tatcgacaac atcagcgcct tcagcggaat
    53401 cagtagattt gaaggttttt tctacgcgag taccggtcag cagacgacgc agtttaacgc
    53461 gagcaaatgc ctggccttta cccggtttta cgaattcact cgcttcaacc gcgtaaggtt
    53521 cgccgtctaa catgatttta agaccagcac gaaaatcgtt gctatagtac gttgccataa
    53581 ggccctctga aatttgttaa ttggtagcta agccacaaaa tggcgcatat tgtaacccta
    53641 aataccccat ccagagaaga ttggttaacg caacttgccg atgttgtgac cgatcctgat
    53701 gaacttctgc gtcttttgaa tatagacgcg gacgaaaaac tgttggccgg acgcagcgcc
    53761 aaaaagctgt ttgccctgcg tgtgccccgc tcatttatcg atcgcatgga gaaaggcaat
    53821 ccggacgatc ctcttttgcg tcaggtactt acctcgcaag atgagtttgt cgtcgtgtcc
    53881 ggattctcca ccgacccgct ggaagaacag cacagcgtag tgcctggttt gttgcataaa
    53941 taccacaacc gggcgctttt gctggtcaaa ggcggctgcg cggtaaattg ccgctattgc
    54001 ttccgtcgcc acttccccta tgccgaaaat cagggcaaca agcgtaactg gcagactgct
    54061 cttgagtatg ttgctgcgca tccggaactg gacgagatga ttttctccgg cggcgatccg
    54121 ctgatggcga aagatcacga gctggactgg ttgctcacac aactggaagc catcccgcat
    54181 atcaaacgtc tgcggattca cagccgtctg ccgattgtga tcccggcacg tatcaccgag
    54241 gcgctggttg aacgctttgc ccgttctacg ctgcaaatct tgctggtgaa tcacatcaat
    54301 catgccaatg aggtagatga aacgttccgt caggcgatgg ctaaattgcg ccgtgtcggt
    54361 gtcaccctgc ttaaccagag cgttctgtta cgtggtgtga acgataacgc acaaacgctg
    54421 gcaaacctga gtaatgcgtt gttcgatgcc ggcgtaatgc cgtattacct gcatgtgctc
    54481 gataaagtac agggcgcggc gcattttatg gtgagtgatg acgaagcacg gcagattatg
    54541 cgtgagttgc tgacactggt gtcgggttat ctggtgccga aactggcgcg agaaatcggc
    54601 ggcgaaccca gcaaaacgcc gctggatctc cagctacgcc agcagtaact gattaaacag
    54661 ggaagttaaa cattagcctg atgcgctacg cttatcaggc ctacattgtg cattgaaatt
    54721 gaatggattt gcaatgaact gtaggccgga taaggcgttt acgccgcatc cggcaagagc
    54781 aacaaacgca agtacagcaa cctgtcatta aactgggaaa tttaatcttt gcccgttgca
    54841 taacctttaa ttattcatat acattgctgt tgttactctt tatatagcca acaccaataa
    54901 ttaacctttc ttttctgttt atatagagtg agtacggcta tttatgttat tttaacaact
    54961 attactacca agcccattta tctggcttat aaattaaaat taattattta attgagaaat
    55021 ggttagggag aacctacatg gccataagca tcaagggtgt taataccggt gttattcgca
    55081 agagtaataa tttcattgca ctggcgctga aaattaaaga acctcgcaat aaagaatcgt
    55141 tgttctttat gtctgtcatg gaactgcgcg acctgctgat tgcgctggaa agtcgtatgc
    55201 atcaaaagca taaactggat gctgctgcac gccttcagta tgagcaagct cgcgataaag
    55261 tcattaagaa aatggcagaa aacatcccag aaattctggt tgatgagctt aaaaatgccg
    55321 atattaatcg tcgggttaac acgctggaac ttaccgataa tcaaggcgaa aatcttacct
    55381 tcgtattaac gctacatgac ggtagcaaat gtgagttagt cgtcaatgag ttgcagattg
    55441 aaatgctggc gcgagcaatc attcatgcaa ttaataatgc ggaaatgcgt gaactggcat
    55501 tacgtattac ctcactgtta gatttcctgc cgttatatga cgttgactgc caggaaaatg
    55561 gcaatctgga atatgacacc tattcccaac cggaatggaa acataacctg ttcgaccatt
    55621 atctggcggt gctttaccgc tttaaagatg aaagtggcaa agaacaattt agcggcgcag
    55681 tggtgaaaac gcgtgaagca acgccgggta aagaaataga ggcgatcact cgccgaatgc
    55741 ttgatttcag ccaacgactg aaaaaactcg cgggtgtgcc atgtcaggtc tatgttcgca
    55801 ccgtagcggc gaataacgcc caaccgctaa cccaggatca atgcttacgc gcgctgcatc
    55861 acttacgtgt tcaatccacc agcaaaactg caccacaggc gaaataatct catctttgct
    55921 aatgcgaggc tggagccatc cagcctgaaa tgactgataa cgtgatcgtt ggtcatgccg
    55981 gatgcggcgt aaacgcctta tccggcctac aaattctcaa gcattcaatc agttgcaatg
    56041 cctgctgtag gcctgataag cgaagcgcat caggcaatga tacgtttgtc acccagcctc
    56101 tggatcacat cagttcgggc acttataaac ctgaccgata atctggctat ccgtcgggac
    56161 aaaactggac aacattccct gcgacgggct gctgatgcca taaatcacgt taccgcccat
    56221 tgcagccgcc tggttgcgca gatcgtttgc tgcgccgcgc atagaaccgc cctcttctcc
    56281 gtgttgcccg gaaagccagt tgctttgctt acctgtcgca gtaccaatca gctggcactc
    56341 tgcgcctggc tgctcgtcca caatgcgtac actctgaccg gcagcactca attcgttgct
    56401 ggagctacaa cccgccatca gtagcgcggc accgacaatc cctgctaagt attttacgtg
    56461 catgttattc cccataatca atgagctgga cgccactcgc gtcgtccgtg tctgaatctt
    56521 atactagaaa gatgaccaaa agaaaaaccc ccagacattt ctgcccgggg gtttgtttat
    56581 ttctgcgagg tgcagggcaa ttacatcatg ccgcccatgc cacccatgcc gcccataccg
    56641 ccagcagcgc ctaagtcagc tgcatcattt ttcggcaggt cggtaaccat gcactcggtg
    56701 gtgatcatca ggccagccac agaagccgcg tactgcagag cagaacgggt tactttggtt
    56761 gggtccagga tacccatgtc gatcatgttg ccgtattctt cggttgctgc gttgtaaccg
    56821 tagttgccgt cgccgccttt aacggtgtta gcaacaacag acggttcttc gccgcagttc
    56881 aggacgatct gacgcagcgg agcttccatt gcacgcagtg caactttgat acccacgttc
    56941 tggtcttcgt tctgaccacg caggtcagcc agtttagacg ctacgcggat cagcgcaaca
    57001 ccaccaccag caaccacgcc ttcttctacc gcagcacggg tcgcgtgcag ggcgtcttca
    57061 acgcgtgctt ttttctcttt catttcaact tcggtagcag cacctacttt gataactgca
    57121 acgccgcctg ccagtttcgc tacgcgttcc tgcagttttt cacggtcgta gtcagaagtt
    57181 gcttcttcaa tctgctgacg gatctgagca acacggccct ggattgcagc ttcttcaccc
    57241 acgccatcga tgatagtggt ggtgtctttg ttgatcacaa cacgtttagc ctgacccagg
    57301 tcttccaggg ttgctttttc cagctccata ccgatctctt cagagattac ggtaccgcca
    57361 gtcagggttg cgatatcctg cagcatagct ttacgacgat cgccgaagcc cggagcttta
    57421 accgcagcaa ctttcacgat gccacgcatg gtgttaacaa ccagagttgc cagcgcttcg
    57481 ccttcaacat cttcagcgat gatcagcagc ggtttgcctg ctttggcaac agcttccaga
    57541 accggcagca tttcgcggat gttagagatt ttcttgtcag ccagcaggat gaacgggctt
    57601 tccagttcta ctgcgccagt ttccggcttg ttgatgaagt aaggagacag gtagccacgg
    57661 tcgaactgca taccttcaac cacgtccagt tcgtcctgca gaccggtacc gtcttcaacg
    57721 gtgataacgc cttctttacc gactttgtcc atcgcttcag cgatcagttt acctacggtt
    57781 tcgtcggagt tagcggagat ggtaccaacc tgagcaatcg ctttagagtc agagcacggt
    57841 acggacagcg ctttcagttc ttcaactgca gcggtaacgg ctttgtcgat accacgtttc
    57901 aggtccatcg ggttcatgcc cgcagcaaca gctttcagac cctcagtgat gatagcctga
    57961 gccagtacag ttgcagtggt ggtaccgtcg cctgcagcgt cgttcgcttt agaggcaact
    58021 tctttcacca tctgcgcacc catattttcg aacttgtctt ccagttcgat ttcacgagca
    58081 acggaaacac catctttggt gatggtcggt gcaccgaaag atttatccag aactacgtta
    58141 cggcctttcg gaccgagggt aactttcact gcatctgcca gtacgtttac gccgcgcagc
    58201 attttcacac gagcgtcgtt accgaatttt acgtctttag ctgccattat ctttattcct
    58261 taaattcgta tgttcagtgt cgtgcgagga ttacgcttca acaattgcca gaatgtcgct
    58321 ttcggacatg atcaacactt cttcattgtc gatcttctca gatttcacac cgtagccatc
    58381 gttgaaaata acgatgtcgc caactttcac atccagcggc ttcacttcgc cattttcaag
    58441 gatacggcca ttgccgacag ccagcacttc gccgcgggtg gatttagccg ctgcagagcc
    58501 ggtcagaacg atgccgccag cagatttagt ttcaacttct ttacgcttga cgatcacgcg
    58561 atcatgcaat ggacgaatat tcattgataa ctctcctttg agaaagtccg tatctgttat
    58621 gggtgacgcc ggaccttaca tggtttcccg gctggtgacc agagaaatgg ggatgaggct
    58681 tcgccccttc aagggggaaa aagaaaaaaa attctgatca gcacaaaatc gggtgaaaac
    58741 cctgattcac ctcacatttc atcgcaattt cttcatcgcc gtcataagcg aatctgattg
    58801 tgctaccatc gaaaatctac gcagttgccc aaaatttggg cgcaatcgga catcaaccca
    58861 tgagtggact caaacaagaa ctggggctgg cccagggcat tggcctgcta tcgacgtcat
    58921 tattaggcac tggcgtgttt gccgttcctg cgttagctgc gctggtagcg ggcaataaca
    58981 gcctgtgggc gtggcccgtt ttgattatct tagtgttccc gattgcgatt gtgtttgcga
    59041 ttctgggtcg ccactatccc agcgcaggcg gtgtcgcgca cttcgtcggt atggcgtttg
    59101 gttcgcgact tgagcgagtc accggctggt tgtttttatc ggtcattccc gtgggtttgc
    59161 ctgccgcgct acaaattgcc gccgggttcg gccaggcaat gtttggctgg catagctggc
    59221 aactgttgtt ggcagaactc ggtacgctgg cgctggtgtg gtatatcggt actcgcggtg
    59281 ccagttccag tgctaatcta caaaccgtta ttgccggact tatcgtcgcg ctgattgtcg
    59341 ctatctggtg ggcgggcgat atcaaacctg cgaatatccc cttcccggca cctggtaata
    59401 tcgaacttac cgggttattt gctgcgttat cagtgatgtt ctggtgtttt gtcggtctgg
    59461 aagcatttgc ccatcttgcc tcggaattta aaaatccaga gcgtgatttt cctcgcgcat
    59521 tgatgattgg tctgctgctg gcaggattag tctactgggg ctgtacggta gtcgtcttac
    59581 acttcgacgc ctatggtgaa aaaatggcgg cggcagcatc gcttccaaaa attgtagtgc
    59641 agttgttcgg tgtaggagcg ttatggattg cctgcgtgat tggctatctg gcctgctttg
    59701 ccagtctcaa catttatata cagagcttcg cccgcctggt ctggtcgcag gcgcaacata
    59761 atcctgacca ctacctggca cgcctctctt ctcgccatat cccgaataat gccctcaatg
    59821 cggtactcgg ctgctgtgtg gtgagcacgt tggtgattca tgctttagag atcaatctgg
    59881 acgctcttat tatttatgcc aatggcatct ttattatgat ttatctgtta tgcatgctgg
    59941 caggctgtaa attattgcaa ggacgttatc gactactggc ggtggttggc gggctgttat
    60001 gcgttctgtt actggcaatg gtcggctgga aaagtctcta tgcgttgatc atgctggcgg
    60061 ggttatggct gttgctgcca aaacgaaaaa cgccggaaaa tggcataacc acataatccg
    60121 gcgtttcgac attaatcctg gcgatcgtct ttatgatcaa ggcggtcgcg ctcatcatcc
    60181 tttcgctggt actcaccatc aaaagtatta ccgccaccgg tcccggcgct aaaaccgccg
    60241 cctggcatac gagaaaagcg caaatgcggc atcaacttca ctgtcagatg cttttgcacc
    60301 ggcggtaata aaagcagaag accgaggaag tcggtgaaaa agcccggtaa taaaagcagc
    60361 aaaccggcaa tgatcagcga aacactttta atcatctccg ccgctgggtt ttcacccgcc
    60421 gccatttttt gctgcatcag cacgaaattc ttaaagccct ggttacgtac cagtgacatc
    60481 ccgataactg acgtgaatat aaccagcacg agggtcagca atacccccaa tacatgggca
    60541 acctgaataa agattgaaat ctcaatatag acataaagga aaatggcaat aaaaggtaac
    60601 cagcgcaaag gtttctcctg taatagcagc cggtaaaacc cggctacctg aatgggttgc
    60661 gaatcgcgtt cagcttatat tgtggtcatt agcaaaattt caagatgttt gcgcaactat
    60721 ttttggtagt aatcccaaag cggtgatcta tttcacaaat taataattaa ggggtaaaaa
    60781 ccgacactta aagtgatcca gattacggta gaaatcctca agcagcatat gatctcgggt
    60841 attcggtcga tgcaggggat aatcgtcggt cgaaaaacat tcgaaaccac atatattctg
    60901 tgtgtttaaa gcaaatcatt ggcagcttga aaaagaaggt tcacatgtca aacaacattc
    60961 gtatcgaaga agatctgttg ggtaccaggg aagttccagc tgatgcctac tatggtgttc
    61021 acactctgag agcgattgaa aacttctata tcagcaacaa caaaatcagt gatattcctg
    61081 aatttgttcg cggtatggta atggttaaaa aagccgcagc tatggcaaac aaagagctgc
    61141 aaaccattcc taaaagtgta gcgaatgcca tcattgccgc atgtgatgaa gtcctgaaca
    61201 acggaaaatg catggatcag ttcccggtag acgtctacca gggcggcgca ggtacttccg
    61261 taaacatgaa caccaacgaa gtgctggcca atatcggtct ggaactgatg ggtcaccaga
    61321 aaggtgaata tcagtacctg aacccgaacg accatgttaa caaatgtcag tccactaacg
    61381 acgcctaccc gaccggtttc cgtatcgcag tttactcttc cctgattaag ctggtagatg
    61441 cgattaacca actgcgtgaa ggctttgaac gtaaagctgt cgaattccag gacatcctga
    61501 aaatgggtcg tacccagctg caggacgcag taccgatgac cctcggtcag gaattccgcg
    61561 ctttcagcat cctgctgaaa gaagaagtga aaaatatcca acgtaccgct gaactgctgc
    61621 tggaagttaa ccttggcgca acagcaatcg gtactggtct gaacacgccg aaagagtact
    61681 ctccgctggc agtgaaaaaa ctggctgaag tcactggctt cccatgcgta ccggctgaag
    61741 acctgatcga agcgacctct gactgcggcg cttatgttat ggttcacggc gcgctgaaac
    61801 gcctggctgt gaagatgtcc aaaatctgta acgacctgcg cttgctctct tctggtccac
    61861 gtgccggcct gaacgagatc aacctgccgg aactgcaggc gggctcttcc atcatgccag
    61921 ctaaagtaaa cccggttgtt ccggaagtgg ttaaccaggt atgcttcaaa gtcatcggta
    61981 acgacaccac tgttaccatg gcagcagaag caggtcagct gcagttgaac gttatggagc
    62041 cggtcattgg ccaggccatg ttcgaatccg ttcacattct gaccaacgct tgctacaacc
    62101 tgctggaaaa atgcattaac ggcatcactg ctaacaaaga agtgtgcgaa ggttacgttt
    62161 acaactctat cggtatcgtt acttacctga acccgttcat cggtcaccac aacggtgaca
    62221 tcgtgggtaa aatctgtgcc gaaaccggta agagtgtacg tgaagtcgtt ctggaacgcg
    62281 gtctgttgac tgaagcggaa cttgacgata ttttctccgt acagaatctg atgcacccgg
    62341 cttacaaagc aaaacgctat actgatgaaa gcgaacagta atcgtacagg gtagtacaaa
    62401 taaagaaggc acgtcagatg acgtgccttt tttcttgtga gcagtaactt aaaaataaca
    62461 acctaatatc aacttgttaa aaaacaagga aggctaatat gctagttgta gaactcatca
    62521 tagttttgct ggcgatcttc ttgggcgcca gattgggggg aataggtatt ggttttgcag
    62581 gcggattggg ggtgctggtt cttgccgcta ttggcgttaa acccggtaac atcccgttcg
    62641 atgtcatttc cattatcatg gcggttatcg ccgctatttc tgccatgcag gttgctggcg
    62701 gtctggacta tctggttcat cagacagaaa agctgctgcg ccgtaacccg aaatacatca
    62761 cgatcctcgc accgatcgtg acctatttcc tgactatttt tgctggtact ggcaacatct
    62821 ctctggcgac actgccagtt atcgctgaag ttgcgaagga acaaggcgtt aaaccttgcc
    62881 gtccgctgtc tactgcagtg gtatccgcgc agattgcgat caccgcatcg ccaatctcag
    62941 cggcagtggt ttacatgtct tccgtgatgg aaggtcatgg catcagctac ctccatctgc
    63001 tctccgtggt catcccgtcc accctgctgg cggttctggt gatgtccttc ctggtcacta
    63061 tgctgttcaa ctccaaactc tctgacgatc cgatttatcg caagcgtctg gaagagggcc
    63121 tggttgaact gcgcggtgaa aagcagattg aaatcaaatc cggtgcaaaa acgtccgtct
    63181 ggctgttcct gctgggcgta gttggcgtgg tgatctatgc aatcatcaac agcccaagca
    63241 tgggtctggt tgaaaaaccg ctgatgaaca ccaccaacgc aatcctgatc atcatgctca
    63301 gcgttgcaac tctgaccacc gttatctgta aagtcgatac cgacaacatc ctcaactcca
    63361 gcaccttcaa agcaggcatg agcgcctgta tttgtatcct gggtgttgcg tggctgggcg
    63421 atactttcgt ttccaacaac atcgactgga tcaaagatac cgctggtgaa gtgattcagg
    63481 gtcatccgtg gctgctggcc gtcatcttct tctttgcttc tgctctgctg tactctcagg
    63541 ctgcaaccgc aaaagcactg atgccgatgg ctctggcact gaacgtttct ccgctgaccg
    63601 ctgttgcttc tttcgctgcg gtgtctggtc tgttcattct gccgacctac ccgacgctgg
    63661 ttgctgcggt acagatggat gacacgggta ctacccgtat cggtaaattc gtcttcaacc
    63721 atccgttctt catcccgggt actctgggtg ttgccctggc cgtttgcttc ggcttcgtgc
    63781 tgggtagctt catgctgtaa tgaccaatcg cggggcgttc acgccccgct ttctttcccg
    63841 ccgactaaca tccttccccc gtccgttgta tagtgacctc tctcttgcgg ttccatctgt
    63901 tcttgcgagg tgtttatgct tgatgaaaaa agttcgaata ccgcgtctgt cgtggtgcta
    63961 tgtaccgcac cggatgaagc gacagcccag gatttagccg ccaaagtgct ggcggaaaaa
    64021 ctggcggcct gcgcgacctt gatccccggc gctacctctc tctattactg ggaaggtaag
    64081 ctggagcaag aatacgaagt gcagatgatt ttaaaaacta ccgtatctca ccagcaggca
    64141 ctgctggaat gcctgaagtc tcatcatcca tatcaaaccc cggaacttct ggttttacct
    64201 gttacacacg gagacacaga ttacctctca tggctcaacg catctttacg ctgatcctgc
    64261 tactttgcag cacttccgtt tttgccggat tattcgacgc gccgggacgt tcacaatttg
    64321 tccccgcgga tcaagccttt gcttttgatt ttcagcaaaa ccaacatgac cttaatctga
    64381 cctggcagat caaagacggt tactacctct accgtaaaca gatccgcatt acgccggaac
    64441 acgcgaaaat tgccgacgtg cagctgccgc aaggcgtctg gcatgaagat gagttttacg
    64501 gcaaaagcga gatttaccgc gatcggctga cacttcccgt aaccatcaac caggcgagtg
    64561 cgggagcgac gttaactgtc acctaccagg gctgtgctga tgccggtttc tgttatccac
    64621 cagaaaccaa aaccgttccg ttaagcgaag tggtcgccaa caacgcagcg ccacagcctg
    64681 tgtctgttcc gcagcaagag cagcccaccg cgcaattgcc cttttccgcg ctctgggcgt
    64741 tgttgatcgg tattggtatc gcctttacgc catgcgtgct gccaatgtac ccactgattt
    64801 ctggcatcgt gctgggcggt aaacagcggc tttccactgc cagagcattg ttgctgacct
    64861 ttatttatgt gcaggggatg gcactgactt acacggcgct gggtctggta gttgccgccg
    64921 cagggttaca gttccaggcg gcgctacagc acccatacgt gctcattggc ctcgccatcg
    64981 tctttacctt gctggcgatg tcaatgtttg gcttgtttac tctacaactc ccctcctcgc
    65041 tgcaaacgcg cctcacgctg atgagcaatc gccaacaggg cggctcaccc ggcggagtgt
    65101 ttattatggg ggcgattgcc ggactgatct gttcaccatg caccaccgca ccgcttagcg
    65161 cgattctgct gtatatcgcc caaagcggga acatgtggct gggcggcggc acgctttatc
    65221 tctatgcgtt gggcatgggc ctgccgctga tgctaattac cgtctttggt aaccgcttgc
    65281 tgccgaaaag cggcccgtgg atggaacaag tcaaaaccgc gtttggtttt gtgatcctcg
    65341 cactgccggt cttcctgctg gagcgagtga ttggtgatat atggggatta cgcttgtggt
    65401 cggcgctggg tgtcgcattc tttggctggg cctttatcac cagcctaaag gccaaacgcg
    65461 gctggatgcg cgtggtgcaa ataatcctgc ttgcagcggc attggttagc gtgcgcccac
    65521 ttcaggattg ggcatttggt gcgacgcata ccgcgcaaac tcagacgcat ctcaacttta
    65581 cacaaatcaa aacggtagat gagttaaatc aggcgctcgt tgaagccaaa ggcaaaccgg
    65641 tgatgttaga tctttatgcc gactggtgcg tcgcctgtaa agagtttgag aaatacacct
    65701 tcagcgaccc gcaggtgcaa aaagcgttag cagacacggt cttacttcag gccaacgtca
    65761 cggccaacga cgcacaagat gtggcgctgt taaagcatct taatgtcctt ggcctaccga
    65821 caattctctt ttttgacgga caaggccagg agcatccaca agcacgcgtc acgggcttta
    65881 tggatgctga aaccttcagc gcacatttgc gcgatcgcca accgtgaacg acacttgcag
    65941 tgggaaagac ggaggaaaat agcgtgcaac gtgaagatgt actgggagaa gccctgaaat
    66001 tattagaatt acaagggatt gccaacacca cgctggagat ggttgctgaa cgtgtggatt
    66061 atccactgga cgagctacgc cgcttctggc cagacaaaga ggcaatcctc tacgatgcgc
    66121 tgcgttatct tagccaacag atagatgtct ggcgtcgtca gctgatgctg gacgaaacgc
    66181 aaaccgccga acaaaagctg ctggcacgtt atcaggcgtt atcggagtgc gttaaaaaca
    66241 accgctatcc gggctgtcta tttatcgctg cctgtacgtt ttatcccgat cctggccacc
    66301 ctattcatca actggccgat cagcaaaaaa gcgcggccta cgatttcacc cacgaactgt
    66361 taaccacgct ggaagttgac gatccggcga tggtagcaaa gcagatggaa ctggtgctgg
    66421 aaggctgttt aagccgaatg ctggtgaatc gtagccaggc ggatgtcgac actgcacatc
    66481 ggctggcgga agatatcctg cgcttcgccc gctgccgcca gggtggcgca ttgacctgac
    66541 agaaacacag aaaagaagcg atttgccgca atcttaagca gttgaatcgc ttttactgaa
    66601 attaggttga cgagatgtgc agattacggt ttaatgcgcc ccgttgcccg gatagctcag
    66661 tcggtagagc aggggattga aaatccccgt gtccttggtt cgattccgag tccgggcacc
    66721 actttcactt cctgattcat ctcccgaact ccacgaaact caagtataat cacatcgttg
    66781 caagcaatct gaacattctg aactccacca gactctactg aactctctct caatcaagcg
    66841 ttcattgggg gccccggtgg gggctcattg tgttcagtca gtttcaggag cccccatatg
    66901 gcacttacag ccagacaggt cgaaacagcc agacctaaag aaaaagacta taaactctct
    66961 gacgaacgtg gtttatatct gctggtaaaa accacgggag cccgctactg gcggcttaaa
    67021 taccggatag caggaaaaga gaaaaaactg gccctcggcg tctatcccga cgtctccctt
    67081 gctgaggcca gaatcaaacg cgacgatgcc cgaaaaatca tctccgaagg tggtgacccg
    67141 ggcgaaaaga agcgaaagga aaaactcact cagaaaatct ctgccaccaa tacgttccat
    67201 gccctcgcta cggaatggca ccagcataaa tctttgtcat ggtctgaaag ttacgccaga
    67261 agcgtactgg aagcgctgga taaagatatt ttcccgtatc tgggcaaacg aagcgttacg
    67321 gatatcctcc cgctggaaat gctggaaatt ctgcgccgca tagaaaaacg tggctcgctg
    67381 gaaaaactgc gtaaggtccg tcaatactgt aatcagattt ttcgttatgc catcgccacc
    67441 ggacgagcca ctgtcaatcc ggcatctgaa ctgaccagta cactggcggc gccaaaagcg
    67501 gcgcatttcc cccacctgag agcagatgag ctccctgttt ttctcaggaa gctcgctgaa
    67561 tatcatggca gtccagttac ccgcatggcg acaaacctgc tgcttctgac tggcctcaga
    67621 accattgaac tacggtctgc tgaatggtca gaaattgatt ttgataatgc cctgtggaca
    67681 atccctgaaa gccgcatgaa aatgcgacgt aaacatgtcg taccactgtc acgacaggtc
    67741 actgacattc tgcaacaact caaaactttc tccggacaat accggctggt tttcccggga
    67801 cgttgtgata tcaacaagcc aatgagcgaa gccagcatca atatggtgct caaacgtatc
    67861 ggttacgatg gcagagcaac cggtcatggt tttcgtcaca ccatgagtac cattctgcac
    67921 gaacaggggt ttaattctgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    67981 atcaggggta cctataatca tgccctgtat attgatggtc gccgtgaaat gatgcagtgg
    68041 tatgctgact acattgattc gctttctgag caggtaagtt aagctgagaa cacgttattc
    68101 tgattttcac ttacttcacg attattcctc cggcctgcag gttcggagga ttcttatttt
    68161 tatgttttat tttcagataa agtttttgac aaaacgttta ttctgcatca gcaaaattat
    68221 cacaaaacaa cgcaatcaca gacaaattaa catttttcag acgggtatat tgagtgccca
    68281 caatgtaaga caggcaggca tccggtgaca tggtaaaata ttaatccagg gcaaaaacaa
    68341 ggcttgatac acaacatcat ggcttttatg ctctcagact atgccaccac agattttaca
    68401 ggctcagaaa atgaatgaca gattacgctt tgaagtgaac gataaccagg gctgtttcgt
    68461 gttcccggac gcctggtttg gctcgttgct ggatgagttt gacgaactaa ttgatgcata
    68521 cgacacagat gaaatcagcg aaacaagtta tatcaacaaa ctcagacgcc ttgcccgaca
    68581 ggagcctgac tttattgatg tacacgctta cctggcatac gcatttctgg agcagaatgc
    68641 cccgcgtaag gcactgaacg ctgctcttaa aggactggca gcaggtaatc gtctcatccc
    68701 ggaaagtttc agtggtgaaa tcatctggat gcatcccgaa aaccggcctt atctgcgcgc
    68761 gctttatgca gcaattctcg caaatgtgca cttacagcgt catcaggatg cagctctgct
    68821 cactgataaa atactggcat acaatcctga agacaatcag ggagcacgct ggctactggg
    68881 accggaactg ttacgtactg gcgaccatga acaggcacgc catgtactga aagcgcatgc
    68941 agatgaattt tcaccttact ggtatgaact cggccttctg cattttctta atggggagct
    69001 ggtaaaagct gcaactgcat ttcgtcgcgg ctttgcggca aatacttaca tcgcagaaat
    69061 actgtgcgga aatctgcatc catttccact tgctgtatgg cataactttt caggcggtcc
    69121 ggatactgct gaagattatt atgccacata ccacccgtta tgggggcaat atccggaagc
    69181 attgctgttt gtaaactggc tctataatca ttcctctgtg ctgcatgaac gggcagaaat
    69241 cattaaatgc gccgaaatgc tgatgcagga agatgatttc gaaatatgcg aaagtatttt
    69301 aagacagcag gagaagttgc gtgaaagaat tgatgagacg ctttctgaga aaattgtaca
    69361 gaaatgcaga aatatgaatg gtgaatatgt ctggccctgg atattgccgt tttcagcggc
    69421 aggcatgaaa catactggca tacagtatca gtagatattg cattagtgta tcctgcacac
    69481 aagtaataat ttatccacca ataataacac tgttaatgtc cccttcccct ggttgtcagc
    69541 caggggttat cttctgaata tttcttttga aaaggataac acagtaaatt atttttatga
    69601 attatcccat ggactcatta acaccctttc ataatgcttt attgtcaaac acgttatggc
    69661 tgacatcaaa aaaaccggat ttcctctgcc agcgggtaat cacctccccg gtgttttcgg
    69721 ttggtctggt tactccagtc tggtaattag caagataatc gatataaaca gtggaaaact
    69781 catcgtacat aatctggtga tgaacattac gcttattttc ccctgaccgg aagaatcaga
    69841 ggctgcggtt tcagactgtc tgccggtaca ttcctctccc cgttaaaaac cataacgggt
    69901 tcattatctt cgtctgtcag cggattgaat ggcggtatat tttcagtacg aatgtcggtc
    69961 agccactgaa aaatacctgc gaaatgacgg gcactgattt tcctgctgac ggactgatga
    70021 gacgtgatgt cactggcggt aataatcagg ggaacgctgt agcctccctg cacatgacca
    70081 tcatgatgaa caggattggc actatcgctg accgacagcc catggtcaga aaagtaaagc
    70141 atggcaaaat gacgggaatg ccggcgaagg ataccatcaa gctgaccgag aaagttatcc
    70201 agtttactga tgctggcgag gtaacaggca atctttcggg gatactgctc caggtaatga
    70261 ttcggccagg agttaagtcg gtcacacgga ttcggatgag agccaatcat gtgcaggaaa
    70321 atcaccttcg gagaggattt atccgccagc gcacgttctg tttcctgtaa caacaacatg
    70381 tcatccgttt tacgggaagc gaagctgcct ttcttgagga aaacggtatg ctccgcatca
    70441 gaagcaataa cagagatgcg tgtgtcatgc tctcccagtt ttccctgatt ggatatccac
    70501 catgtgctgt atcctgcttt tgctgccagc gccaccacgt tgttgccgga atcagggttc
    70561 tgctcatagt cataaatcag tgtccggctc agggaaggta cggtactggc tgctgccgat
    70621 gtatagccgt caataaataa accgggagcc gtattcagcc acggtgtggt tggtacggga
    70681 taaccatata ccgacatata atccctgcgc acactctcac cagtgacaat cacaatcgtg
    70741 tcatacaacg gtgctcccgg caggattttc cagctgtcag ccctgtgctg actcagttgt
    70801 tcataacgct gcatttcacg caatgtgtca gttgtcccca caacggttcc tttaaccatc
    70861 cgcaacggcc agctgtttac agagcataat acgaacagca gcagtgccag ccagttacgg
    70921 tgaccgcggt ggtgtgtccg ccagaaaata accatgaaca ccagaatcgc ggcactgacc
    70981 agaaaatgat aaacaggaat catcccggta aactccgcag cctcatccgc cgtggtctgc
    71041 aacaacgcga caataaaact gttgttgatt ttaccgtacg tcatacccgc aggcgcatac
    71101 agtgcacaac agaacagaaa taacagagct gtaatggatg taagggtatt tctgtgtgca
    71161 aggagcagaa gaaggaacag cagcaacaca ttcccggtgg cattcttctc agtgtatccg
    71221 catgcaattg tggttatgac agaaataaca aaaaagaata aaaacaataa aatccgtggt
    71281 gtgttgtccg gacaaaacag ttttctgata ttcatcggag tatatcggca acattattat
    71341 gaagagaata aaataataag aaaaacagaa atatcctgta aagcagataa cagacaaccc
    71401 tgcagtataa ttacggcagg gagtttccgt ttaattacag aagacaggta attattatct
    71461 ttcaggagta gtttcgtcag aagttataag tcacaccaag catgatgtca tgacttttaa
    71521 catcaacttc tgatttatat ttatcgcccc actcatcctt gtaactcaca ctggcatcac
    71581 ctgcatcaag atagcgatag ctgaggtcca gagcgatatc cggggttacg tcatagcgga
    71641 cacctgcccc aaaactccat gcaaaattgt tatcatatcc cgggtaatgc aacgcagaaa
    71701 aatcgctcac ctcaccagat ttattccagg aggtatcagt atatgttgcc ttatgatgta
    71761 tccgtgcata acccagtcca acagacaccc atggagtgaa tgcactgctg tttctgaagt
    71821 cataatacgt attcaccatc agagtattca cactgagcct gttctgtgtg tcttcccgac
    71881 caccaccgac catcggagag taccatgtat ccagtgtata acgggagtcc gcagctccgc
    71941 ggccatagaa ttccacttcc gtgcgcacag gaacattgta atgctgataa aaatcatagc
    72001 ccaccgcaag gccggcaccg aataccgtat cgttcttatc gccgccctta tacttataaa
    72061 catcgtcccc aaagtcctga caaaagcgct ggtcagtctg agtcacaacc gaagcaccag
    72121 ctttaccggt catataaaag cctgttttgc ctctatcagc cagggcctgg cctgaaaaca
    72181 tgcccaccac agcaagcgct gaaaccgcaa taatcttttt catttcagtt atctccgcta
    72241 tcactttccc cgaacgataa acgtggttgc ttattctaaa gctatgcaaa agacagggta
    72301 aaaagagaaa aatcgatcga atcgatcgac aaaatcgatc atgcatgatg aagatttctt
    72361 atcgaataca taaaaatagt gatggcaatc gtggcaggag cttacgtcag gccctgaagt
    72421 actgacacag gcattgatgg caggtgatat aaggagtgct ccagggagtg actcccgact
    72481 gacccaggca ctgtcccgtt gtcaacccgt atggttactg ctctgactca tttcctgtcg
    72541 tcagacgttc aactctggac cactggttat gacgacaggt gtcgctcatt tgtcagcact
    72601 ataacggtca aagatcgtgg cattgacaca ttctccagag gtaaatttct caagaggtaa
    72661 caactctgcc aggaggatat ctgcagagag ttacattcgt ttgggttcat atctgtgccg
    72721 cgcgttttcc ttccatcagt gaacaaacat tcagtcccat gacgaccggg caaatagcgt
    72781 gttcacagat attgctgtcc aggtacgaag taccataatg gatattggta agttattggg
    72841 tgaaccccct ccatgaaaaa accaaatata aaaatgaatg tttcgggaga gacttcactc
    72901 gctttattga tagtgattgt ttatctttta ctgaagacag gatatttcag atgtgatatc
    72961 cctgtaacat attgaccagc aatacttttt atttgtacca ctggaggata ttgtgctgaa
    73021 aaatttacgg ctgcatattc tgttcctgct ttttatctca gtgtcagtca gggcggaaaa
    73081 accatggtat tttgatgcaa ttggtctgac agaaacaacc atgtcgctca cagacaaaaa
    73141 taccccggtt gtcgtatctg ttgttgattc cggagtggca tttgtaggag gcctcagcga
    73201 cagcgaattt gccaaattca gttttactca ggatggttcg ccattcccgg taaaagagcc
    73261 tgaagcttta tatattcatg gtactgccat ggcttcactc attgcctcac gtcatggggt
    73321 ttatggtgtt tatcctcatg cactgatatc cagcagaagg gttattcctg acggtgtaca
    73381 ggactcatgg cttagagcaa ctgaaagcat tatgtcaaat gtttttcttg caccaggaga
    73441 agagaaaatc attaatatat cgggaggcca gaaggggata tcttccgcat cggtctggtc
    73501 cgaactgctt tcccgtatgg gccggaataa tgagcgatta attgttgcgg cagtgggtaa
    73561 tgatggcgca gatatacgaa aactgagtgc tcagcagaga atatggcctg cggcttacca
    73621 tccggtcagc agtgtgaata aaaagcagga tcctgtgata agagtggcag ctctggcaca
    73681 gtaccggaaa ggagaaacac ctgtattgca tggtggaggg gtcaccggaa gtcggttcgg
    73741 aaacggctgg gttgatattg ccgcaccggg acagaatatt acattcctca gaccagatgg
    73801 aaaaacgggg atcggtagtg gaacctcgga agcaacagct atagtttccg gcgttctggc
    73861 agcgatggtc tcatgtaatc cccgtgcaac agcgacagaa ctgaagcgta cactgctgga
    73921 gtctgcagac aaatacccct cacttgcaga taaggtgacg gaagggagag ttcttaatgc
    73981 agaaaaagca attagcatgt tctgcaagaa aaat
//
LOCUS       DAZGSP010000008        72254 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZGSP010000008 DAZGSP010000000
VERSION     DAZGSP010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.008"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..469)
                     /gene="sulA"
                     /locus_tag="ACHK74_000623"
     CDS             complement(<1..469)
                     /gene="sulA"
                     /locus_tag="ACHK74_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFP0219499.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATR"
     gene            688..1317
                     /gene="sxy"
                     /locus_tag="ACHK74_000624"
     CDS             688..1317
                     /gene="sxy"
                     /locus_tag="ACHK74_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFP0219500.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAIVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(1280..3442)
                     /gene="yccS"
                     /locus_tag="ACHK74_000625"
     CDS             complement(1280..3442)
                     /gene="yccS"
                     /locus_tag="ACHK74_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFP0219501.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(3452..3898)
                     /gene="yccF"
                     /locus_tag="ACHK74_000626"
     CDS             complement(3452..3898)
                     /gene="yccF"
                     /locus_tag="ACHK74_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFP0219502.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIMLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            4021..6075
                     /gene="helD"
                     /locus_tag="ACHK74_000627"
     CDS             4021..6075
                     /gene="helD"
                     /locus_tag="ACHK74_000627"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFP0219503.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(6107..6565)
                     /gene="mgsA"
                     /locus_tag="ACHK74_000628"
     CDS             complement(6107..6565)
                     /gene="mgsA"
                     /locus_tag="ACHK74_000628"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFP0219504.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(6661..7323)
                     /gene="yccT"
                     /locus_tag="ACHK74_000629"
     CDS             complement(6661..7323)
                     /gene="yccT"
                     /locus_tag="ACHK74_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFP0219505.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            7496..7909
                     /gene="yccU"
                     /locus_tag="ACHK74_000630"
     CDS             7496..7909
                     /gene="yccU"
                     /locus_tag="ACHK74_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFP0219506.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(7954..8271)
                     /gene="hspQ"
                     /locus_tag="ACHK74_000631"
     CDS             complement(7954..8271)
                     /gene="hspQ"
                     /locus_tag="ACHK74_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFP0219507.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(8329..9519)
                     /gene="rlmI"
                     /locus_tag="ACHK74_000632"
     CDS             complement(8329..9519)
                     /gene="rlmI"
                     /locus_tag="ACHK74_000632"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFP0219508.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            9614..9892
                     /gene="yccX"
                     /locus_tag="ACHK74_000633"
     CDS             9614..9892
                     /gene="yccX"
                     /locus_tag="ACHK74_000633"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFP0219509.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLIEPHHPSGELADFRIH"
     gene            complement(9889..10218)
                     /gene="tusE"
                     /locus_tag="ACHK74_000634"
     CDS             complement(9889..10218)
                     /gene="tusE"
                     /locus_tag="ACHK74_000634"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFP0219510.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(10309..10968)
                     /gene="yccA"
                     /locus_tag="ACHK74_000635"
     CDS             complement(10309..10968)
                     /gene="yccA"
                     /locus_tag="ACHK74_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFP0219511.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(11175..11262)
                     /locus_tag="ACHK74_000636"
     tRNA            complement(11175..11262)
                     /locus_tag="ACHK74_000636"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11226..11228),aa:Ser,seq:tga)
     gene            11689..12807
                     /gene="hyaA"
                     /locus_tag="ACHK74_000637"
     CDS             11689..12807
                     /gene="hyaA"
                     /locus_tag="ACHK74_000637"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFP0219512.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            12804..14597
                     /gene="hyaB"
                     /locus_tag="ACHK74_000638"
     CDS             12804..14597
                     /gene="hyaB"
                     /locus_tag="ACHK74_000638"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFP0219513.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            14616..15323
                     /gene="hyaC"
                     /locus_tag="ACHK74_000639"
     CDS             14616..15323
                     /gene="hyaC"
                     /locus_tag="ACHK74_000639"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFP0219514.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            15320..15907
                     /gene="hyaD"
                     /locus_tag="ACHK74_000640"
     CDS             15320..15907
                     /gene="hyaD"
                     /locus_tag="ACHK74_000640"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFP0219515.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            15904..16302
                     /gene="hyaE"
                     /locus_tag="ACHK74_000641"
     CDS             15904..16302
                     /gene="hyaE"
                     /locus_tag="ACHK74_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFP0219516.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            16299..17156
                     /gene="hyaF"
                     /locus_tag="ACHK74_000642"
     CDS             16299..17156
                     /gene="hyaF"
                     /locus_tag="ACHK74_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFP0219517.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAVTQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            17290..18834
                     /gene="appC"
                     /locus_tag="ACHK74_000643"
     CDS             17290..18834
                     /gene="appC"
                     /locus_tag="ACHK74_000643"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFP0219518.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQALRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            18846..19982
                     /gene="appB"
                     /locus_tag="ACHK74_000644"
     CDS             18846..19982
                     /gene="appB"
                     /locus_tag="ACHK74_000644"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP0219519.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            19995..20087
                     /gene="cbdX"
                     /locus_tag="ACHK74_000645"
     CDS             19995..20087
                     /gene="cbdX"
                     /locus_tag="ACHK74_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFP0219520.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            20167..21465
                     /gene="appA"
                     /locus_tag="ACHK74_000646"
     CDS             20167..21465
                     /gene="appA"
                     /locus_tag="ACHK74_000646"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFP0219521.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(21580..23760)
                     /gene="etk"
                     /locus_tag="ACHK74_000647"
     CDS             complement(21580..23760)
                     /gene="etk"
                     /locus_tag="ACHK74_000647"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFP0219522.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(23780..24226)
                     /gene="etp"
                     /locus_tag="ACHK74_000648"
                     /gene_synonym="yccY"
     CDS             complement(23780..24226)
                     /gene="etp"
                     /locus_tag="ACHK74_000648"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFP0219523.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(24214..25353)
                     /gene="gfcE"
                     /locus_tag="ACHK74_000649"
     CDS             complement(24214..25353)
                     /gene="gfcE"
                     /locus_tag="ACHK74_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFP0219524.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(25399..27495)
                     /gene="gfcD"
                     /locus_tag="ACHK74_000650"
     CDS             complement(25399..27495)
                     /gene="gfcD"
                     /locus_tag="ACHK74_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP0219525.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(27495..28241)
                     /gene="gfcC"
                     /locus_tag="ACHK74_000651"
     CDS             complement(27495..28241)
                     /gene="gfcC"
                     /locus_tag="ACHK74_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP0219526.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(28238..28882)
                     /gene="gfcB"
                     /locus_tag="ACHK74_000652"
     CDS             complement(28238..28882)
                     /gene="gfcB"
                     /locus_tag="ACHK74_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFP0219527.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(28989..29294)
                     /gene="gfcA"
                     /locus_tag="ACHK74_000653"
     CDS             complement(28989..29294)
                     /gene="gfcA"
                     /locus_tag="ACHK74_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HFP0219528.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(29736..29948)
                     /gene="cspH"
                     /locus_tag="ACHK74_000654"
     CDS             complement(29736..29948)
                     /gene="cspH"
                     /locus_tag="ACHK74_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFP0219529.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            30234..30446
                     /gene="cspG"
                     /locus_tag="ACHK74_000655"
     CDS             30234..30446
                     /gene="cspG"
                     /locus_tag="ACHK74_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFP0219530.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            30457..30645
                     /gene="ymcF"
                     /locus_tag="ACHK74_000656"
     CDS             30457..30645
                     /gene="ymcF"
                     /locus_tag="ACHK74_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP0219531.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            30620..30850
                     /gene="ymcE"
                     /locus_tag="ACHK74_000657"
     CDS             30620..30850
                     /gene="ymcE"
                     /locus_tag="ACHK74_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFP0219532.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            30840..31013
                     /gene="gnsA"
                     /locus_tag="ACHK74_000658"
     CDS             30840..31013
                     /gene="gnsA"
                     /locus_tag="ACHK74_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFP0219533.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(31061..32134)
                     /gene="yccM"
                     /locus_tag="ACHK74_000659"
     CDS             complement(31061..32134)
                     /gene="yccM"
                     /locus_tag="ACHK74_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP0219534.1"
                     /translation="MAENKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLVTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(32206..34950)
                     /gene="torS"
                     /locus_tag="ACHK74_000660"
     CDS             complement(32206..34950)
                     /gene="torS"
                     /locus_tag="ACHK74_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFP0219535.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QRQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            35033..36061
                     /gene="torT"
                     /locus_tag="ACHK74_000661"
     CDS             35033..36061
                     /gene="torT"
                     /locus_tag="ACHK74_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFP0219536.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQFATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(36034..36726)
                     /gene="torR"
                     /locus_tag="ACHK74_000662"
     CDS             complement(36034..36726)
                     /gene="torR"
                     /locus_tag="ACHK74_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFP0219537.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            36856..38028
                     /gene="torC"
                     /locus_tag="ACHK74_000663"
     CDS             36856..38028
                     /gene="torC"
                     /locus_tag="ACHK74_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFP0219538.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            38028..40574
                     /gene="torA"
                     /locus_tag="ACHK74_000664"
     CDS             38028..40574
                     /gene="torA"
                     /locus_tag="ACHK74_000664"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFP0219539.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            40571..41170
                     /gene="torD"
                     /locus_tag="ACHK74_000665"
     CDS             40571..41170
                     /gene="torD"
                     /locus_tag="ACHK74_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFP0219540.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVVRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(41263..41568)
                     /gene="cbpM"
                     /locus_tag="ACHK74_000666"
     CDS             complement(41263..41568)
                     /gene="cbpM"
                     /locus_tag="ACHK74_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFP0219541.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(41568..42488)
                     /gene="cbpA"
                     /locus_tag="ACHK74_000667"
     CDS             complement(41568..42488)
                     /gene="cbpA"
                     /locus_tag="ACHK74_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFP0219542.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            42748..44004
                     /locus_tag="ACHK74_000668"
                     /pseudo
     CDS             42748..44004
                     /locus_tag="ACHK74_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
     gene            44297..45538
                     /gene="agp"
                     /locus_tag="ACHK74_000669"
     CDS             44297..45538
                     /gene="agp"
                     /locus_tag="ACHK74_000669"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFP0219543.1"
                     /translation="MNKTLIAAAVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPYTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(45576..45803)
                     /gene="yccJ"
                     /locus_tag="ACHK74_000670"
     CDS             complement(45576..45803)
                     /gene="yccJ"
                     /locus_tag="ACHK74_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFP0219544.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(45824..46420)
                     /gene="wrbA"
                     /locus_tag="ACHK74_000671"
     CDS             complement(45824..46420)
                     /gene="wrbA"
                     /locus_tag="ACHK74_000671"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP0219545.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            46793..46966
                     /gene="ymdF"
                     /locus_tag="ACHK74_000672"
     CDS             46793..46966
                     /gene="ymdF"
                     /locus_tag="ACHK74_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP0219546.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(47049..48376)
                     /gene="rutG"
                     /locus_tag="ACHK74_000673"
                     /pseudo
     CDS             complement(47049..48376)
                     /gene="rutG"
                     /locus_tag="ACHK74_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
     gene            complement(48397..48891)
                     /gene="rutF"
                     /locus_tag="ACHK74_000674"
     CDS             complement(48397..48891)
                     /gene="rutF"
                     /locus_tag="ACHK74_000674"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFP0219547.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(48902..49492)
                     /gene="rutE"
                     /locus_tag="ACHK74_000675"
     CDS             complement(48902..49492)
                     /gene="rutE"
                     /locus_tag="ACHK74_000675"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFP0219548.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDRSKLFARLPRLSFEEACGLL"
     gene            complement(49502..50302)
                     /gene="rutD"
                     /locus_tag="ACHK74_000676"
     CDS             complement(49502..50302)
                     /gene="rutD"
                     /locus_tag="ACHK74_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFP0219549.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSITQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(50310..50696)
                     /gene="rutC"
                     /locus_tag="ACHK74_000677"
     CDS             complement(50310..50696)
                     /gene="rutC"
                     /locus_tag="ACHK74_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFP0219550.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(50708..51400)
                     /gene="rutB"
                     /locus_tag="ACHK74_000678"
     CDS             complement(50708..51400)
                     /gene="rutB"
                     /locus_tag="ACHK74_000678"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFP0219551.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(51400..52491)
                     /gene="rutA"
                     /locus_tag="ACHK74_000679"
     CDS             complement(51400..52491)
                     /gene="rutA"
                     /locus_tag="ACHK74_000679"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFP0219552.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            52779..53417
                     /gene="rutR"
                     /locus_tag="ACHK74_000680"
     CDS             52779..53417
                     /gene="rutR"
                     /locus_tag="ACHK74_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFP0219553.1"
                     /translation="MTQGAVKTTGKRSRTVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(53457..57419)
                     /gene="putA"
                     /locus_tag="ACHK74_000681"
     CDS             complement(53457..57419)
                     /gene="putA"
                     /locus_tag="ACHK74_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP0219554.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAAQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEAILSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNHNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            57474..57683
                     /locus_tag="ACHK74_000682"
     CDS             57474..57683
                     /locus_tag="ACHK74_000682"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219555.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKINQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            57842..59350
                     /gene="putP"
                     /locus_tag="ACHK74_000683"
     CDS             57842..59350
                     /gene="putP"
                     /locus_tag="ACHK74_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFP0219556.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            59893..60723
                     /gene="efeU"
                     /locus_tag="ACHK74_000684"
     CDS             59893..60723
                     /gene="efeU"
                     /locus_tag="ACHK74_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFP0219557.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            60781..61908
                     /gene="efeO"
                     /locus_tag="ACHK74_000685"
     CDS             60781..61908
                     /gene="efeO"
                     /locus_tag="ACHK74_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFP0219558.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            61914..63185
                     /gene="efeB"
                     /locus_tag="ACHK74_000686"
     CDS             61914..63185
                     /gene="efeB"
                     /locus_tag="ACHK74_000686"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFP0219559.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            63804..64592
                     /gene="phoH"
                     /locus_tag="ACHK74_000687"
     CDS             63804..64592
                     /gene="phoH"
                     /locus_tag="ACHK74_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFP0219560.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(64642..65055)
                     /gene="pgaD"
                     /locus_tag="ACHK74_000688"
     CDS             complement(64642..65055)
                     /gene="pgaD"
                     /locus_tag="ACHK74_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFP0219561.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(65057..66382)
                     /gene="pgaC"
                     /locus_tag="ACHK74_000689"
     CDS             complement(65057..66382)
                     /gene="pgaC"
                     /locus_tag="ACHK74_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFP0219562.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            complement(66375..68393)
                     /gene="pgaB"
                     /locus_tag="ACHK74_000690"
     CDS             complement(66375..68393)
                     /gene="pgaB"
                     /locus_tag="ACHK74_000690"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFP0219563.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            complement(68402..70825)
                     /gene="pgaA"
                     /locus_tag="ACHK74_000691"
     CDS             complement(68402..70825)
                     /gene="pgaA"
                     /locus_tag="ACHK74_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFP0219564.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            71412..>72254
                     /locus_tag="ACHK74_000692"
     CDS             71412..>72254
                     /locus_tag="ACHK74_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HFP0219565.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFG
                     GPIVAHILSSHYESYNLHIAELTNENGKVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKNI"
BASE COUNT        17709 a        18095 c        18732 g        17718 t
ORIGIN      
        1 gtctcgtggc gtgagaggat gcgcttaccg gacgcataat aaaccccata gcgttacctt
       61 catttgccgc atcaacaagt tcagcatgct cttcttcagt caaatcatct gccaaccaac
      121 cgatcaccac actgtaattg cccgtgcgta aagcgcgaac cattgactcc acagtgtggc
      181 aaggggagag ctggctaatc tgcattactt tcgttaaggg tagcccagat gcctgaaccc
      241 attcccgact cagtttttgt tgcggtgtta accagagttg ccagcgcgat tgctgaccga
      301 gttgctgtaa caatggcaac agtagaagtt gcgtcatcat gggctgatct tcgcgataga
      361 caacttcact gataagcccg gctgtagtgt tttccgtaga gacacgcgca attttacttg
      421 ctgcggatga gaacgacgaa gaacgatgtg catagcctga agtgtacata atcaatccag
      481 cccctgtgag ttactgtatg gatgtacagt acatccagtg acaacaaaga tcaaccctat
      541 tttcggaaag agcctcgcaa attttgtcgt tggtgacggg aaaacataaa ttaatcttgc
      601 cccttaagaa taagttgcct attttcgtag ttaacggatc cgttaatgtg aatcattttt
      661 ttatgttatg attttaaaag gaattttatg aaaagcctct cctataagcg gatctataaa
      721 tcacaagaat acctggcaac gttgggcaca attgaatacc gatcattgtt tggcagttac
      781 agcctgaccg ttgacgacac ggtgtttgcg attgtttctg atggtgagtt gtatcttcgg
      841 gcttgtgagc aaagtgcaca gtactgtgta aaacatccgc ctgtctggct gacatataaa
      901 aagtgtggcc gatccgttac cctcaattac tatcgggttg atgaaagtct atggcgaaat
      961 caactgaagc tggtgcgtct gtcgaaatat tctctcgatg cagcgctgaa agagaaaagc
     1021 acgcgcaata cccgggaaag actgaaagat ttgcccaata tgtcttttca tctggaagcg
     1081 attctcgggg aggtggggat taaggatgta cgggcgttac gtatacttgg ggcaaaaatg
     1141 tgttggttgc gactgcggca gcaaaacagt ctggtgacag aaaagattct gtttatgctt
     1201 gaaggtgcca ttatcggcat tcatgaagct gcgctcccgg tggcacgccg ccaggagctt
     1261 gcagaatggg ctgactctct tacgccgaaa caggagtttc ctgcggaact tgagtaatct
     1321 ggcgttgcag acgaccaatc tcaggcagta atgcaatcaa taatccaact tgttgtacga
     1381 ccagaggttc tttgctgtct gcccgtggtt caagttgctg catccgctgt ttcaggctag
     1441 ctaatgcctc attgacgcgt tcttcatcag caggttgatg atgtaacgcg tcatcaacat
     1501 agcaaactgc gtcatcaaga aacgccagaa tttcaggatt agttaactgc tcccggtgag
     1561 caccgagggc tgagatatag ctggtaaacg tatggttaag gcacagcaac cgaaacgcgg
     1621 cttcgcgaat ttgcggggta acgttcggct cgctggacat atttgatacc accgacgcca
     1681 gctcagcatc acggttgtgt gcatcgcggc gggcaatacg atacgccaga cggttatcac
     1741 gcccctgatg gtattgctcc agtatggcat cgagataccg acagttggcc tctgtggcgc
     1801 gttcgagcat gcgcggcaga ttgcgaaact gccagtcagg ccagatgtag ctcactgccg
     1861 cccacgcaat ggcacaacca atcagcgtat cgattacgcg aggtaacgct acttcaaaac
     1921 cttcacccag taagttaaaa cacagtagca ccaaaagtgt gatgaacatc gttgcatgag
     1981 cgtattgcac gttacggaag gcaaaaaaga gcacgccggt aataaccagc agcaccagct
     2041 gcccttccag tgatggcaca aaccacagca caggaatgcc aatggcgata cctaccagcg
     2101 taccaataat ccttaacttc aggcggtggc gcgtggcgtt atagtttggc tggcagacaa
     2161 acaaacttgt cagcaagatc caatacccgt gatgcattcc ggttatttga atgatggcgt
     2221 agccgaagca caacaccagc gacattctta ccgcatgacg gaagagggcg gattccggcg
     2281 tgaagtgacg gctaagacgc agccagatat cactcaaccc gtgcgggctg tcatcagcga
     2341 gctcattttc atcattatta tggggtagtg cctgggcctg ttctgattca attgttgcca
     2401 gttgggcatc aatggcgcgt aaattgttca gcaaaaatcc cagtgttttg agtaaatcgg
     2461 cgggtgcgcc gttatcgcgc atccgctcca gcgcagcatc aatatgcgta aaagcgcgct
     2521 caaaatgcgg atcatgttga taaggctgac gcaacaaaat acagcgtgac agttgctggc
     2581 acgcctggcc ctgcatcgac atcagccgct gaaaacggaa cagcacgtcg ctgtggcgaa
     2641 aatgttcacg caatgtttga tactgaatat gagaagagct ggcacgctcg tgaatatcct
     2701 gtgcgacaaa gtaataatgc agcgtgcgac gcgttccccg ttgaccacga tcgccacgta
     2761 agcgggtcag cagcgagagt ttcgtctgat tcaatgtcgc catcagcaga ccgttggcga
     2821 gagccaaatc gtacagcggt gcctggcttt catcttcaat atcaggatca aacatgcgcg
     2881 acttgagctc aagataacgc gccagttgtt catagcaacg cgccaggttg tcctgcagcg
     2941 ggcggaccgg gaacagcaga tgaccaataa gtgtcaggac gttgtaccag acggcaccgg
     3001 ccagcagata catcggctgc tgataccagt gctcatacag tgatgttccc aacatagtgt
     3061 aaatggcgat cagcaatgca ccgaaggcaa ttgttgcata gcgttgaccc agaccgccga
     3121 gcaaaatgaa gccgctggta gagagcgtta agccaatcgc aaatagccag ggccagggaa
     3181 acagcaattc tactgaggcc gaggcgataa aaaagcagaa cagcgtaatg atgaggttac
     3241 gcaaacgtcc cgccagtcgg tcatcgagat cggtcagcgc cgctgccacc atccccagcg
     3301 ttagcggaat cgtcagtttt acatcaccca gccaccacgg aaacgctgtg gttccacaaa
     3361 gcgcaataaa aatacgcgcg taatacagcc aggcgctgtt ccaggtatag cgtttgagca
     3421 aaggacttag cataaaggcc atattagtgg attattcaaa acgacgacgt gcattggctt
     3481 cacgcgcagc ttgcgctgtt tctaccgata ccacgcgacg accaaccggc catagtgcaa
     3541 tagcggcaat tttaaagttc gcaatgccga caggaatgcc aatgattgaa atacattgtg
     3601 cgatgcccgt tgcaatgtgc atcaggcata accaccagcc aaagaaaatc agccagaaaa
     3661 tattcaatac cgtaccgcca gtattcagca gcacattttt gccagccggg ttcagttcat
     3721 cgacatgaat agcttcattg ccataaggca ccagagacag tttagtgatc tcccagcagg
     3781 atcgtgtcag cggtaaggta aaaatcagca taatactgac cagagtcgcc aacagccagc
     3841 ccagagtggt ggcaaatccg ccaagcacaa agttcagaat gttcaaaacg gtacgcataa
     3901 aacctcgctt tactgtggtt ttcagtaatg ggcggcaatt gtaacgtttt tttgggctgg
     3961 agcacgtttt ctctgacggt tacactgata agaaataatt tcgtgtggat ctacagagtc
     4021 atggaactga aagcgacaac gcttggaaaa cgtctggcac agcaccctta cgatcgggcg
     4081 gtgatcctca atgccgggat taaagtctcc ggcgatcgcc acgaatacct tattcctttc
     4141 aatcaattac tggcgattca ctgtaagcgc ggtctggtat ggggcgagct ggaatttgta
     4201 ctgccggacg aaaaagtggt gcgtctgcac ggcaccgaat ggggcgagac gcagcgtttt
     4261 taccatcatc ttgatgctca ctggcggcgg tggagtggcg agatgagcga aattgcgtct
     4321 ggtgttttac gccagcaact ggatttgatt gccacgcgca ctggagaaaa taaatggctg
     4381 acgcgtgagc aaacctctgg cgtgcagcaa caaatccgcc aggctttgtc ggcgttgccg
     4441 ttgccggtta accgactgga agaattcgat aactgccgtg aggcgtggcg taaatgtcag
     4501 gcctggttga aagatattga aagcgctcgg ttgcagcata accaggcgta taccgaagcc
     4561 atgcttaccg agtatgcgga ttttttccgc caggtcgagt cttcaccgct gaatccggcg
     4621 caggcccggg cagtcgttaa tggcgagcat tctctgttag tgctggcagg tgcaggaagc
     4681 ggaaaaacgt cggtgctggt ggcccgtgca ggctggttgc tggcgcgtgg tgaagcgtcc
     4741 cctgagcaaa ttttattgct ggcgtttggt cgcaaagccg ctgaagagat ggacgagcgg
     4801 attcgcgaac ggctacatac cgaagacatt accgcacgca cgtttcatgc gctggcgctg
     4861 catattattc agcagggcag caaaaaagtt ccgatagtca gcaaactgga aaatgatacc
     4921 gctgcccgtc atgaactctt tattgctgag tggcgcaagc aatgcagcga aaagaaagcg
     4981 caggcgaagg gctggcggca atggctgacg gaagaaatgc agtggtcagt gccagaaggt
     5041 aacttctggg atgatgaaaa attacagcgt cgccttgcct ctcgcctcga tcgctgggta
     5101 agtctgatgc ggatgcacgg tggtgcacag gcagaaatga ttgccagtgc acccgaagag
     5161 attcgcgatc tgttcagtaa acgtatcaag ttgatggccc cgttattaaa agcctggaaa
     5221 ggtgcgctga aggcagaaaa cgctgtcgat ttttcgggcc ttattcatca ggcgattgtg
     5281 attctggaga aaggtcgctt tatcagcccg tggaagcata ttctggttga tgaatttcag
     5341 gatatctcgc cgcagcgggc agcgttgtta gcggcattac gcaagcaaaa cagtcagacg
     5401 acgttgttcg ccgttggtga tgactggcag gcgatttacc gattcagcgg tgcgcaaatg
     5461 tcgctcacca ccgctttcca tgaaaacttt ggtgaaggcg atcgctgtga tttagacacg
     5521 acttaccgtt ttaacagtcg tatcggtgag gtggcaaacc ggtttattca gcagaaccca
     5581 ggccagttga aaaagccgct aaacagctta accaatggag acgaaaaagc cgtcacgtta
     5641 ttggatgaga gtcaacttga cgctttgctg gataagctct ctggttatgc caaaccggaa
     5701 gagcgcattc ttatcctggc gcgttaccat cacatgaggc ctgccagcct ggaaaaagcg
     5761 gcaacacgct ggccgaagtt gcaaatcgac tttatgacca ttcatgccag caaagggcaa
     5821 caggcggatt acgtcatcat cgttggcttg caggagggga gtgatggttt tccggctgcg
     5881 gcgcgggagt cgattatgga agaggcgcta ctgccaccgg ttgaggattt cccggacgct
     5941 gaagaacggc ggttaatgta cgtggcgctg acccgggcac gccatcgggt atgggcactg
     6001 tttaacaaag agaatccctc tccctttgtg gaaatactga aaaatctgga tgtgccggta
     6061 gcgagaaaac cgtaagaaac aggtggcgtt tgccacctgt gcaatattac ttcagacggt
     6121 ccgcgagata acgctgataa tcggggatca gaatatcgac cgcgtcgttg aaatgcggcg
     6181 actggattat gaagtctgcc gttgccacgt tggtggcgac cggaatgttc cataccgtcg
     6241 ccagacgcaa caaggctttc acgtcaggat cgtgcggcac ggcatttagt gggtcccaga
     6301 agaaaatcaa tacatcaatt ttcccttctg agatcaatgc gccaacctgc tggtcacccc
     6361 ccattgggcc actcaacatc gcgttgacgt tcatgccggt cgcgcgggaa attaagttac
     6421 cggtagtgcc tgttgcatac agtacgtgtt gttccagtaa cggttgatgc cgttccaccc
     6481 agctcatcag catttgtttg cagtgatcgt gtgccaccag cgcaatatgt ttccgcgcag
     6541 gtaaagtgcg agtcgtcagt tccataatgt acatccgtag ttaactttcc tacagattac
     6601 tgtaagcact tatcgctgca agataaagac cgaaaaagcc tgcgcacagg cacaaaaatc
     6661 tcaggaagat ggttgttttt ccgcccattg taggaaagta tttcgcgttt gtgggtcagc
     6721 cagtttaaac caatacttca gccgttgttc tgtgagcacc tgagactgcg gtggaatagc
     6781 atccagctcg gaaacacccg aaagcagcgt actgtcatct gccatcatcg tggcaaattg
     6841 cggtagtgaa gcgcgtttcg cggatttgtt atagcgttca acctcgacct cataatcaat
     6901 cgtttttgcc gttgaggtaa tggcgagaat atccagcttt accggaatcg gcgtcgcatc
     6961 gccatccaac aattcaaggc gcggcgcggc atcaaaatgg ttagcttccc gctcattctc
     7021 caggcgaggc aggcgaaaat tgacctggtt gatgagctgg gtattaaaac tgaccaccag
     7081 cggtggggag atatacagcc gttcttcact attggaaaga tgaatcgtct tctcaacgcg
     7141 aaacactaac tgatgcggtc cattatccag ttcaatgctg tccgcaccgc gcagcagaga
     7201 gctggaaacc ttcttgccgt cgagtaccag aagatcgaca tcggttgaaa gccgcagcgt
     7261 ggtggcaaaa acggataccg gcaaacataa tgcaatcaag gtggtcacga tgccggtttt
     7321 cataggtagc tcctgtcaaa agaccgcccg aataataatg tgtcaaaatt tttctttaat
     7381 catacttact aacatataga catatttccc gcttttgctc tcattcattc gtattagctg
     7441 catggttggc atgtcggctt tgtcgtacac tttgttaaac agtctggagg aaataatgaa
     7501 agaaaccgat attgcaggca ttttaacgtc cactcacacc attgcgctgg tgggggcaag
     7561 cgacaaaccc gatcgtccca gctatcgcgt gatgaaatat ctactcgacc aggggtatca
     7621 cgtcattccg gtttcgccaa aagttgccgg caaaacgctg ctgggccagc aggggtatgg
     7681 cacgctggca gacgttccgg aaaaggtcga tatggtcgat gttttccgta attcagaagc
     7741 ggcgtggggc gtggcacagg aagctattgc cattggcgcg aaaaccctct ggatgcaact
     7801 gggggtgatt aatgagcaag cggcagtact ggcacgggat gccgggttga atgtggtgat
     7861 ggatcgctgc ccggctattg agatccctcg cctgggcctg gccaaataaa aaatccccgg
     7921 aaggcaaaaa ccttccgggg attcgttcag ggatcagtta cgcagacgcg gggcctggag
     7981 ttgtttgcgg atggtctgcg ccagctcatc catggatggc tgttcaggat gttcatcctg
     8041 caattcgcta cttagctggg cttcagccag gtaggtatga accggtaggc cgttatcgtc
     8101 ctccatcact acgtgatacc acggagcggc gcgaagctcg tcatttaccg ccagctcatc
     8161 aggcgacggt tcagagagcg aataaaccgg gtcgatatcc acgaccactc cgagataacc
     8221 taacagggaa tggcggacct gctggccgat accgaatttg ctggcaatca tagtcccctc
     8281 ccgggaaatc tttacctttg atataagggt aaaattccac atttcaagtt acatgacgcg
     8341 acaggcaaac cctttcagat atagcccttc cggataggta gcgatcaccg gatgatcggc
     8401 tgcctgacgg aactgctcta taaattgtac atcacggccg gcatcaattg cggcatccgc
     8461 gatgattttc tgaaataaat cgctggtcat cagaccggaa caggagaaag tcaggagaat
     8521 accgccttca ttcagcaact gaatcgccag catgttgata tctttatagc cacgacacgc
     8581 gcccatcaac tggcttttat tctcaacaaa cttcggcggg tccatcacga taacgtcaaa
     8641 tttttcaccg cgatcgcgat aagtacgcag caatttaaag acatcatcac ggacaaactc
     8701 agccttgctc agatccagtt tgttcagctc aacgttctgc cgtgcaatat ccagcgcttc
     8761 ctgggaggta tcaacgctga caacctggct gcaaccgccc atcagtgccg atacggcgaa
     8821 accaccggta taggagaaac agttcagcac acgtttattt tcaacgtagc ggcgggtagc
     8881 caggcggctg tctcgctggt ccaggtagta gcccgttttg tgtccgtgct gaatatccac
     8941 cagcagcttc atgccgtgtt cttcaatcgg cagcagggca ggcggcaact cgccggtgac
     9001 gaggccctgg gtcagctcca tcccttcttt tttacgtacc gcaacatcgc tgcgatcgta
     9061 aatcgcacat tccgggtaca gcgtttgcag ggcactaatt aatgccgcgc gctgatattc
     9121 tgccccagca ctcagcagtt gcagcaccag aaaattaccg aaacgatcga tagtaatacc
     9181 cggcaggcca tcagattctc cggcgattaa acgatagctg tcgaggccat ctttttgcgc
     9241 cagccagtca cgccattttt gtgcttgttg caaacggcgg gaaaaaaaag caatgtcgat
     9301 agactcagac gggtcaaacg tccagacgcg cgcccggatt tgcgaagctg gcgaataagc
     9361 gccgcgtgct aaccattttc cctgatgatc aacaatatcg atggtttcac cgaggctggc
     9421 tttaccttcc atgcgggcaa cggccccgga aaagacccac ggatggcgac gaagtaatga
     9481 tttttcgcgc cctttggcta acactaaacg tacactcata atttactttt ctgtcgatgc
     9541 aaagaaatgg gcgtcattgt ccggagtttt ccatggattt gcaacgcgtc cattaaggat
     9601 aaggagagca gcaatgtcga aagtctgcat aattgcctgg gtttacgggc gagttcaggg
     9661 cgtaggattt cgctacacca cacagtacga agcgaaaaga ctgggtttaa ccgggtacgc
     9721 caaaaatctt gacgatggca gcgtggaagt ggttgcctgc ggtgaagaag ggcaggtgga
     9781 aaaattaatg cagtggttaa agagtggcgg cccgcgttct gcgcgtgtgg aacgggtgct
     9841 tatcgagccg catcatccct cgggggaatt agccgatttt cgtatccatt aaatacattt
     9901 taccggtttt ggcaggccag caattttggt ggcttgctta gccggacctt tcggaaacag
     9961 tcgatataaa tagcggctgt tacctttttc ttcgccaaat ttattcgcca tcgcttttac
    10021 cagcatacga atcgccggag aagtattgaa ttccagatag aaatcacgca caaaacgcac
    10081 cacttcccag tgttctggcg acagcgaaat cccttcgttc tctgcaatca ccacagccag
    10141 cggctcactc cactggctgc tttctttgag atagccttcg gtatccgttt ctatctcttt
    10201 accttcgaag atcagcataa ttattactac cttaatcaga ctggcggcag tgtaacaaaa
    10261 aacaaagccc cgcataaagc gaggctatga aagtgttagc gggtgagatt aatcgcggct
    10321 agcgaagccc agaatgctca gcaggctgac gaagatgttg tacagcgaaa catacaggct
    10381 aaccgtggca cgaatatagt tcgtctcacc gccatgaatg atgttgctgg tttcaaacaa
    10441 gatagcgcca gaggagatca gaatgaagac cgcgctgatc gccagatgca gagcaggcag
    10501 ctgcaggaag atattcgcaa ccataccaat cagcaccacc acaatacccg ccatcagcat
    10561 accgccgagg aacgacatat ctttgcgggt ggtcagcaca tatgcagagc agcagaagaa
    10621 cactaacgcc gttccgccca gtgccatagc gattacgtca cccattccgg cagacagata
    10681 ggtgttcaga ataggtccga ggatataacc cagaaaaccg gtaaaggcga atgcggagat
    10741 aatcccggtc ggcttattcg ccgttttata ggtcaggaac atcaaaccat acatacccac
    10801 cagcgtcaga atcagacccg gagatggcag catcagcacc gtactggcag ttgcggtaat
    10861 cgccgaaaag gccagcgtca ggctcagcag aaaataggta ttacgcagca ccttatgggt
    10921 gctaagcagt gatgtacggt catgtgaaga actaacaata cgatccattg agtcactctc
    10981 tatgacagat gtaattaatt aagcagcata atgataatgc gtaagggcac ccagaagttt
    11041 tacccatctt tacgcatttg atctggaaca ggtttaacag cggattatca ggtcattaag
    11101 caaatataac gccctgagaa tttcgacagg caaaagaaaa aggggttagc atttagctaa
    11161 ccccttatct tatttggcgg aagcgcagag attcgaactc tggaaccctt tcgggtcgcc
    11221 ggttttcaag accggtgcct tcaaccgctc ggccacactt ccggaatgac gcgcactata
    11281 aacatcccga tgcggcgtgt aaacccctaa tttgtttgtt tgcctgaaaa acagccaaaa
    11341 gtgcattgat agcgtgaaat aacagcagat tgatcatttc atcaccatga attccttctc
    11401 ttttactcgt ttagcaaccg gctaaacatc cccaccgccc ggcaaaaaga aaaataggtc
    11461 catttttatc gctaaaagat aaatccacac agtttgtatt gttttgtgca aaagtttcac
    11521 tacgctttat taacaatact ttctggcgac gtgtgccagt gcagaaggat gagctttcgt
    11581 tttcagcatc tcacgtgaag cgatggtttg ccttgctaca gggacgtcgc ttgccgacca
    11641 taagcgcccg gtgtcctgcc ggtgttgcta ggagaagaga cgtgcgatat gaataacgag
    11701 gaaacatttt accaggccat gcggcgtcag ggcgttaccc ggcgcagctt tctcaaatat
    11761 tgtagtctgg ctgccacgtc gctgggatta ggcgcgggaa tggcaccaaa gattgcctgg
    11821 gcgctggaga acaaaccccg cattccggtg gtatggatcc acggtctgga atgcacctgc
    11881 tgtaccgaat cttttatccg ctccgctcac ccactggcga aggacgtcat cctttccctg
    11941 atttccctcg attatgacga tactttgatg gctgccgccg gaacccaggc ggaagaagtc
    12001 ttcgaagaca tcatcacgca atacaatggc aaatatatcc tcgcagtaga aggtaatccg
    12061 ccgctgggcg agcaggggat gttctgtatc agcagcggtc gaccgtttat tgagaaactc
    12121 aaacgtgccg ctgccggagc cagcgcgatt atcgcctggg gaacctgcgc gtcctggggc
    12181 tgcgtgcagg ccgcgcggcc caatccgacg caggcaacgc ctatcgacaa agtcatcacc
    12241 gacaaaccca ttatcaaagt acctggctgc ccgccgatcc cggatgtgat gagcgccatc
    12301 attacttaca tggtgacctt tgatcgcttg ccagatgtcg acagaatggg ccgtccactg
    12361 atgttttatg gtcagcgtat ccatgataaa tgctatcgcc gcgctcactt cgacgccgga
    12421 gagttcgtcc agagttggga tgatgacgct gcccgcaaag gttactgcct gtacaaaatg
    12481 ggctgcaaag ggcctaccac ctataacgcc tgttcctcca cacgctggaa tgatggcgtt
    12541 tcttttccaa tccagtctgg tcacggctgc ctgggctgtg cggaaaatgg tttctgggat
    12601 cgcggttcgt tctacagccg cgtggtcgat attccgcaaa tgggtactca ttccactgcc
    12661 gataccgtcg gtttaaccgc gcttggcgtg gtggcagcgg ctgttggtgt gcacgcagtc
    12721 gccagcgccg ttgaccagcg cagacgtcat aaccagcaac ctacagaaac cgaacatcag
    12781 ccaggcaatg aggataaaca ggcatgagca ctcagtacga aactcaggga tacaccatca
    12841 ataatgccgg acgccgcctg gtggtcgacc cgattacgcg catcgaaggc cacatgcgct
    12901 gcgaagtgaa tattaacgat cagaatgtga tcaccaatgc cgtctcctgc ggcaccatgt
    12961 ttcgcgggct ggagatcatt ctgcaagggc gcgacccgcg cgatgcgtgg gcgttcgttg
    13021 aacgtatctg cggcgtctgt actggcgtac acgccctggc ttcggtttac gccatcgaag
    13081 atgctatcgg tattaaagtg ccggacaacg ccaatatcat ccgcaacatt atgctggcaa
    13141 cgctctggtg ccacgatcat ctggtgcact tctatcagct tgccgggatg gactggatcg
    13201 atgtgttaga tgcgctgaaa gccgacccgc ggaaaacctc cgaactggcg caaagtctct
    13261 cctcttggcc gaaatcatcc ccgggctatt tcttcgacgt acaaaaccgc ctgaaaaaat
    13321 tcgttgaagg cgggcagttg gggatcttcc gcaatggcta ctgggggcac ccgcagtaca
    13381 agttgccgcc agaagccaac ctgatgggct ttgcccacta cctcgaagct ctcgatttcc
    13441 agcgtgaaat tgtgaaaatc cacgcggtct ttggcggtaa aaacccgcat ccaaactgga
    13501 ttgtcggcgg gatgccttgc gccatcaaca ttgacgaaag cggcgcggtc ggggcagtca
    13561 atatggaacg cctgaacctg gtgcagtcaa ttatcacccg cacggcggac ttcattaaca
    13621 acgtgatgat ccccgacgcc ttagccatcg gtcagttcaa caaaccgtgg agcgaaatcg
    13681 gcactggtct ttctgataaa tgcgttctca gctacggcgc attcccggat attgccaacg
    13741 actttggcga gaaaagtctg ctgatgcctg gcggcgcggt gattaacggc gacttcaaca
    13801 atgtgctgcc agtggatttg gttgatccgc agcaggtgca ggagtttgtc gaccacgcct
    13861 ggtatcgata tcccaacgat caggtcgggc gtcatccgtt cgatggcatc accgacccgt
    13921 ggtacaaccc cggcgatgtc aaaggcagcg ataccaacat tcagcagctg aatgaacagg
    13981 aacgctactc gtggatcaaa gcgccacgct ggcgcggtaa cgcgatggaa gtggggccgc
    14041 tggcgcgcac gttaatcgct tatcacaaag gcgatgctgc gaccgttgag tcggtcgatc
    14101 gcatgatgtc ggcgttgaac ctgccgcttt ccggtatcca gtcaacgtta ggccgcattt
    14161 tgtgccgcgc gcacgaagcg cagtgggccg caggtaagtt gcagtatttc ttcgacaagc
    14221 tgatgaccaa cctgaaaaac ggcaatctcg ccactgcttc cacggaaaaa tgggaacctg
    14281 caacctggcc gacagagtgc cgtggtgtcg gttttaccga agcgccgcgc ggggcgttag
    14341 gccactgggc cgccattcgc gatggcaaga ttgatctcta ccagtgcgtg gtgccgacca
    14401 cctggaacgc cagcccgcgc gatcccaaag ggcagattgg cgcttatgaa gcggcgctga
    14461 tgaacaccaa aatggcgatc cccgagcaac cgctggagat cctgcgtact ctgcacagct
    14521 ttgacccgtg cctcgcctgt tcaacacacg tgctgggcga cgacggtagc gagctgatct
    14581 ccgtgcaggt gcgttaacag cgaaggagaa tcatcatgca acagaaaagc gacaacgttg
    14641 tcagccacta tgtctttgaa gcgccagtcc gcatctggca ctggttgacg gtgttatgca
    14701 tggcggtgtt gatggtcacc ggatacttta tcggcaagcc gctaccttcc gtcagcggcg
    14761 aggcgacgta tctgttctat atgggctaca tcaggttaat tcacttcagc gccgggatgg
    14821 tttttaccgt ggttttgctg atgcggatct actgggcttt tgttggcaat cgatactccc
    14881 gcgagctgtt tatcgtgccg gtatggcgta aaagctggtg gcagggcgtg tggtatgaaa
    14941 tccgctggta tctgtttctg gcaaaacgtc cgagtgccga tataggccat aatcccatcg
    15001 cccaggcggc gatgttcggc tatttcctga tgtcggtctt tatgatcatc actggttttg
    15061 cgctgtacag cgaacacagc cagtacgcta tttttgcgcc gttccgttat gtggtggaat
    15121 ttttctactg gacgggtggc aactcaatgg acattcacag ctggcatcgg ctggggatgt
    15181 ggctgattgg cgcgtttgtg atcggtcatg tctacatggc gctgcgtgaa gacatcatgt
    15241 ccgacgacac ggtgatctcc accatggtca acggctaccg tagccacaaa tttggcaaaa
    15301 taagtaacaa ggagcgttca tgagcgagca acgcgtggtg gtcatggggc tgggcaacct
    15361 gctgtgggcc gatgaaggct tcggcgtgcg ggtggcggaa cggctgtatg cccattacca
    15421 ctggcccgag tatgtggaga ttgtcgatgg cggtactcag ggactgaact tgctggggta
    15481 tgtcgaaagc gccagccatc tgttgattct cgatgccatt gactacgggc tggaacctgg
    15541 aacgctgcga acctatgccg gagaacgcat tccggcttat ctcagcgcga agaaaatgag
    15601 cctgcatcag aacagtttct ccgaagtgtt ggcgctggcg gatatccgcg gacatctgcc
    15661 agcacatatt gccctcgtcg gtctgcaacc cgcaatgctc gacgactacg gcggtagcct
    15721 gagcgaactg gcacgggagc aactgcccgc tgcggaacag gcggcgctgg cgcagctggc
    15781 tgcgtgggga attgtgccgc aaccggctaa tgaatcgcgc tgtctcaatt atgactgtct
    15841 gtcgatggaa aattacgaag gcgttcgctt gcgccagtac cggatgacac aggaggagca
    15901 gggatgagca acgacacgcc atttgatgcg ttgtggcaac gaatgctggc gcgcggctgg
    15961 acgccagtca gtgaatcccg tcttgacgac tggcttacgc aagcgccaga cggcgtggtg
    16021 ttattaagca gtgacccgaa acgcacgcca gaggtcagcg ataatccggt aatgattggc
    16081 gaattactgc gcgagtttcc cgactataca tggcaggtgg cgattgctga ccttgagcag
    16141 agcgaagcca tcggcgatcg ctttggcgtc tttcgctttc ctgccacttt agtgtttacc
    16201 ggcggaaact atcgcggcgt gctgaatggt attcacccgt gggcggaact gataaacctg
    16261 atgcgcgggc ttgtcgaacc gcagcaggag cgtgcctcat gagcgaaact tttttccatc
    16321 tgctggggcc aggaacgcaa ccgaacgatg acagtttcag catgaatcca ctgccgatca
    16381 cctgtcaggt gaatgatgaa ccgagtatgg cggccctgga gcaatgtgct cacagcccgc
    16441 aggtgattgc gctgttaaac gagttacaac atcaactaag cgaacgccaa ccgccgttgg
    16501 gcgaggtgct ggcagtcgat ctgttaaatc tcaacgccga cgatcgtcac tttatcaata
    16561 cgcttctcgg ggaaggggaa gtgtcagtgc gcattcagca ggctgacgac agtgaaagtg
    16621 aaatacagga ggcgatcttc tgcggattat ggcgggtgcg cagacgtcgc ggcgaaaagt
    16681 tgctggagga caaactggag gctggctgcg cgccgctggc gttgtggcag gcggtaacgc
    16741 aaaatctctt gccgacagat tcgctgttac cgccgcccat tgatggcctg atgaatggcc
    16801 taccgttggc gcatgagtta ctggcacatg tacgtaaccc cgacgcgcag ccgcacagca
    16861 ttaatctgac gcaattaccc atcagcgagg ctgatcggct ttttctctca cgtctctgtg
    16921 ggccgggaaa tattcagatt cgtaccattg gctatggcga gagctatatc aacgccacgg
    16981 ggttacgcca tgtctggcat ttacgctgta cggacacctt aaaaggcccg ttactggaaa
    17041 gttatgaaat ctgcccaata ccggaagtgg tgctggcagc gccagaagat ttggtcgact
    17101 ctgcgcagcg gcttagcgag gtatgtcagt ggctggcgga agctgcaccg acgtaaaatg
    17161 acggtaagta tcgctttcag tcttatgaat atcgcaatcg gcgaatacct ctggtcgtag
    17221 agtttcagga taaagaggga gatctaccat tatcgggtta tttttctctc ttcgcctaca
    17281 ggagtgcgca tgtgggatgt cattgattta tcgcgctggc agtttgctct gaccgcgctg
    17341 tatcactttt tatttgtacc ccttaccctg gggctgattt ttttgctggc tatcatggaa
    17401 accatttacg tggtcaccgg caaaacaatc taccgcgata tgacgcgctt ctggggtaag
    17461 ctcttcggta tcaattttgc tcttggcgtg gctaccggcc tgaccatgga gtttcagttt
    17521 ggtactaact ggtcattcta ttccaactat gtgggcgata tttttggcgc accactggcg
    17581 atggaagcat taatggcctt cttcctcgaa tccacctttg tcgggctgtt cttcttcggc
    17641 tggcagcggc tgaataaata ccagcacctg ctggtgacgt ggctggtggc gttcggttca
    17701 aatctctctg cattgtggat attgaatgcc aacggttgga tgcaataccc gaccggtgcg
    17761 cattttgata tcgacaccct gcgtatggag atgaccagct tcagcgagct ggtctttaac
    17821 ccggtcagcc aggtgaaatt tgtgcacacc gtaatggcgg gctacgtgac cggggccatg
    17881 tttattatgg cgatcagcgc ctggtattta ctgcgcggac gggagcgcga tgtcgcatta
    17941 cgttcgtttg ccatcggttc cgtcttcggt actctggcga ttatcggtac cctgcaactc
    18001 ggagacagtt ctgcgtatga agtcgcgcaa gtacaaccgg taaaactggc ggcgatggaa
    18061 ggggagtggc aaacggaacc tgcacctgca ccgttccatg tggttgcctg gccggaacag
    18121 gatcaagagc gtaacgcctt tgccatcaaa attcccgcgc tgctagggat cctcgccact
    18181 cactcattag ataaacccgt gccgggtctg aagaatttga tggctgaaac ctacccacgc
    18241 ttgcaacgcg gacgtatggc ctggctgtta atgcaggaaa tatcgcaagg caatcgtgag
    18301 ccgcatgtgt tgcaggcatt ccgggaactg gaaggtgacc tgggctacgg catgttgctc
    18361 tcccgctatg cgccggatat gaatcatgtc acagccgcac agtaccaggc ggcgatgcgt
    18421 ggcgcgatac ctcaggttgc gccggtattc tggagtttcc gcatcatggt gggctgtggt
    18481 tccctgctgc tactggtgat gctgattgcg cttgtccagg cgctgcgtgg caaaatcgac
    18541 cagcatcgct gggtgctgaa aatggcgctc tggagtttgc cgttgccgtg gattgcgatt
    18601 gaagccgggt ggtttatgac cgagtttggt cgtcagccgt gggcgataca ggacatctta
    18661 ccgacatact ccgcgcactc cgctttaacc acaggacaac tggctttctc actgatcatg
    18721 atcgtagggc tttacaccct gttcttaatc gccgaagtct acctgatgca gaaatatgcc
    18781 cgtctggggc cgagcgcgat gcagagtgaa caaccgacgc agcaacaggg gtagaggaga
    18841 aaatcatgtt tgattatgaa acattgcgct tcatctggtg gctgctgatt ggcgtgatcc
    18901 tggtggtctt tatgatctcc gacggatttg acatggggat cggctgtctg ctgccgctgg
    18961 tggcgcgtaa tgatgatgaa cgccggatag tgataaacag cgttggtgca cactgggaag
    19021 gcaaccaggt ctggttgatc ctcgctggtg gggcattatt tgccgcctgg cccagagtgt
    19081 atgcagcggc gttttccggc ttttatgtgg cgatgatcct ggtgctgtgc tcactgttct
    19141 tccgcccgct ggcctttgat tatcgcggaa aaatcgccga tgcacgctgg cgtaaaatgt
    19201 gggacgccgg tctggtcatc ggcagtctgg tgccgccggt agtcttcggt atcgccttcg
    19261 gcaacttgtt gctcggcgtg ccgtttgcct tcacaccgca attacgcgtg gagtatcttg
    19321 gcagcttctg gcaactgctg acgccattcc ctttattgtg cggattgctc agccttggga
    19381 tggtgatttt gcaaggtggc gtctggttac aactgaaaac tgttggtgtg attcatctgc
    19441 gttcacagct ggccaccaaa cgcgctgcac tgttggtgat gctgtgcttt ttgctggcgg
    19501 gttactggct gtgggtcggt attgatggct ttgtactgct cgcccaggat gctaacggtc
    19561 cttccaatcc gttaatgaaa ctggtggcag tgctacctgg tgcctggatg aataatttcg
    19621 tcgaatcgcc cgttttgtgg atcttcccgc tgctggggtt cttctgccca ttgctgacgg
    19681 tgatggcgat ttatcgtggt cgcccgggtt ggggattttt aatggcgtca ttgatgcaat
    19741 ttggcgtgat tttcacggca ggcatcacgc tgttcccctt tgtcatgccg tcaagcgtga
    19801 gtccaatctc cagcctgacg ttgtgggaca gcacttccag tcagctgacg ctgagcatta
    19861 tgttggtaat cgtgctgata tttttgccca ttgtgttgct ctacactctc tggagctact
    19921 acaaaatgtg gggacgcatg acaacagaaa ctctccgccg taacgaaaac gagttgtact
    19981 aaggagcagg aacaatgtgg tatttacttt ggttcgtcgg cattttgttg atgtgttcgc
    20041 tctccaccct tgtgttggta tggctggacc cgcgtctgaa aagttaacga acgtaggcct
    20101 gatgcggcgc attagcatcg catcaggcaa tcaataatgt cagatatgaa aagcggaaac
    20161 atatcgatga aagcgatctt aatcccattt ttatctcttc tgattccgtt aaccccgcaa
    20221 tctgcattcg ctcagagtga gccggagctg aagctggaaa gtgtggtgat tgtcagtcgt
    20281 catggtgtgc gtgctccaac caaggccacg caactgatgc aggatgtcac cccagacgca
    20341 tggccaacct ggccggtaaa actgggttgg ctgacaccgc gcggtggtga gctaatcgcc
    20401 tatctcggac attaccaacg ccagcgtctg gtagccgacg gattgctggc gaaaaagggc
    20461 tgcccgcagt ctggtcaggt cgcgattatt gctgatgtcg acgagcgtac ccgtaaaaca
    20521 ggcgaagcct tcgccgccgg gctggcacct gactgtgcaa taaccgtaca tacccaggca
    20581 gatacgtcca gtcccgatcc gttatttaat cctctaaaaa ctggcgtttg ccaactggat
    20641 aatgcgaacg tgactgacgc gatcctcagc agggcaggag ggtcaattgc tgactttacc
    20701 gggcatcggc aaacggcgtt tcgcgaactg gaacgggtgc ttaattttcc gcaatcaaac
    20761 ttgtgcctta aacgtgagaa acaggacgaa agctgttcat taacgcaggc attaccatcg
    20821 gaactcaagg tgagcgccga caatgtctca ttaaccggtg cggtaagcct cgcatcaatg
    20881 ctgacggaga tatttctcct gcaacaagca cagggaatgc cggagccggg gtggggaagg
    20941 atcaccgatt cacaccagtg gaacaccttg ctaagtttgc ataacgcgca attttatttg
    21001 ctacaacgca cgccagaggt tgcccgcagc cgcgccaccc cgttattaga tttgatcaag
    21061 acagcgttga cgccccatcc accgcaaaaa caggcgtatg gtgtgacatt acccacttca
    21121 gtgctgttta tcgccggaca cgatactaat ctggcaaatc tcggcggcgc actggagctc
    21181 aactggacgc ttcccggtca gccggataac acgccgccag gtggtgaact ggtgtttgaa
    21241 cgctggcgtc ggctaagcga taacagccag tggattcagg tttcgctggt cttccagact
    21301 ttacagcaga tgcgtgataa aacgccgctg tcattaaata cgccgcccgg agaggtgaaa
    21361 ctgaccctgg caggatgtga agagcgaaat gcgcagggca tgtgttcgtt ggcaggtttt
    21421 acgcaaatcg tgaatgaagc acgcataccg gcgtgcagtt tgtaatgcat aaaaaagagc
    21481 attcagttac ctgaatgctc tgaggctgat gacaaacgaa gaactgtcta atgcgtagac
    21541 cggaaaaggc gttcacgccg catccggcca ctttcagttt tactctttct cggagtaact
    21601 ataaccgtaa tagttatagc cgtaactgta agcggtgctg gcgcgtttaa tcacaccatt
    21661 gaggatagcg cctttaatat tgacgcctgc ctgttccaga cgctgcattg acaaactcac
    21721 ctctttggcg gtgttcaagc caaaacgcgc aaccagcagg ctggtgccaa cagaacgccc
    21781 cacgaccgcg gcatcactca ccgccagcat cggcggcgta tcgacaatca ccagatcgta
    21841 atggtcgttc gcccattcca gtaattgacg catccgatcg cgcatcagca gttcagacgg
    21901 gttaggtggc acctgaccgc gagtaatcac atcaaagcct cctttgccaa aatgctggat
    21961 cactttgttg agctcatctt tacctgccag atattccgac aagccatgtt cattactcac
    22021 ggtaaacagg ttatgcgaat aaccacggcg taagtcggca tcaataaata acactttttg
    22081 atcggactgg gcgatcaccg ctgccagagt tgaactgaca aacgttttac cactgtctgg
    22141 cgtcgcaccg gtgatcatca gaatgttatt ctccgtctcc atcatagcga aatgcagact
    22201 ggttcgtagc gcacgtacgg cttccacagc agaatccgcc gggttatcca ccgccaggaa
    22261 ggggatattt ttagtacgat ggcgctgctg attagaaaat aaatttttct tacgcagacg
    22321 ggtgcgttta tccagccact cggacattgg gatagtggca taaacgctga tgccgtgctc
    22381 ttccagttgt tccggggctt ctacaccacg acgcaacatc gcacgcgcca gcacggcacc
    22441 cacagaaata aacaggccaa gaataaaacc aagcaccaca ttcaacgctt ttttcggttt
    22501 cactggctgc ggctgagtga ctgccgggtc gataatccgc acgttaccaa tggcactgga
    22561 tttcgaaata ctcaactcct gctggcggtt aagtaattgc agatataccg cacggcccgc
    22621 ttctacgtca cgacttaaac gcaacacttc ctgttgggtg gaaggcattg ccgatacccg
    22681 cttattcagg cgtttgcgtt cttgctccag cgtctggcgt ttttccagca gcgcacgata
    22741 agttgggtga tctttcttat acagctggga gatctctgcc tcgcggaaag tcagctcatt
    22801 gagttgatta tcaacgttca caatctgctc aagaacggct ttggcttcca ggttaaggtc
    22861 aaccgaatcg cgctgctggc gataaacgtt gagtttttct tccgcctggt ccagctcgct
    22921 gcgcacttca ggcaactggc gctgtaagaa ttcaaggctt tgtgaatcct gcgccgcctg
    22981 gcgagcgata ttctgttgca aatagttgtt agcgatgctg ttcagaatac gagtaatcaa
    23041 ctggggatca tcaccggtca tggtaagttc cagcatccca ctttctttac tgcgttcgct
    23101 aacggtaaag gtttcctgca acgcgttaat cgcttccagt tcggtacgct ggctcaggac
    23161 aaactgtgtt cctggtttgg ccttaatatc cgcgatagtc agcgcaacgc catctttttc
    23221 cagacgctgg ccgaccatac cattgacggt gaactcttca ccttccagcg tatagtggcc
    23281 gttttcccca accgtgagtg tcagttgctg atcctgacca ttcagttgtg gaatatgcat
    23341 ccagctgatc gccagctcac ctggtttctc tttggttaat ctcgcccagc cgcgacccac
    23401 aatcggaaaa tacttctgct caaccatgtc gcgcagattc agttcagcaa tggttttacc
    23461 gagaatcatg cgcgattgca gcagttggat ctccggtgca gactcgggcg atgagttagg
    23521 gatcatatcg ctcaggccac tgagaatggc gttgccctgt ttctgctcaa cctggaccag
    23581 agtatctgcc tgataaattg gcgtgcttaa cagcgagtaa gcgacagcga tcagcgtgaa
    23641 taacgcggtc acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag
    23701 cagatcgatc tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat
    23761 cgtattttat aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct
    23821 ccaacatacc gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct
    23881 ctttctgttc cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcgattg
    23941 cggtaacctg agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca
    24001 tctcagcagt gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa
    24061 catctgccgc tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga
    24121 cttttacgcc cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt
    24181 tgccggtaca aaccaccagg attgagttaa attttagttg ggccatgttt gaatccaccg
    24241 tgaagtctct gtcagatcat gcaccccgga aatcgtcggt accagcagag aaatcacacg
    24301 gttccaacga gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa
    24361 ttccgttccg agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat
    24421 tttgccattc tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac
    24481 atcctgattc ataccctcag cgttacccag cgcttccgcc agggtcatac cgctgcggtc
    24541 cattttcagc gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt
    24601 gcgcggaata aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg
    24661 ctgcatcagc gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt
    24721 gcgccagtct gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag
    24781 aggaatattg gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt
    24841 ttgcgatcgg aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag
    24901 acgcgctgta atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc
    24961 aatatagggg tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct
    25021 acggtattgc cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac
    25081 atcaccaatc ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc
    25141 ctgcgacata atcggtttgg cgcgcaactg atcaatcaat cctggcgtga cgggatagat
    25201 attgaccatt ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact
    25261 ggtcgataaa ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt
    25321 cagtaccgaa aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat
    25381 gttatgaatt acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggata
    25441 gagctggtat ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac
    25501 caccgcgcgg ttgcggttag gtccaatggt catcaagtcg aatggaatcg agatataaaa
    25561 acctttacta aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca
    25621 tacccctacc gccacgccgc tgtcaaagcg tttggcgacg tcgatcgttg cgcctttatc
    25681 ttttgccaga tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt
    25741 ccagtaagcc gtcacgaaac ctgttggcgt ggaataatcg gtgaaacgca tcatgttgtc
    25801 ccagtcacgc tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg
    25861 gcgataaagc agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac
    25921 ctggccatag aaaccattgc ctaagtcagc aaaatagttc gcctgcaagt tgttgagata
    25981 aacgtcattg cgaacgtagt cacggatatg cgtgcgcacg cggggcaggg tagagtccgc
    26041 gggcaacagc gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgcc
    26101 atcaagcagc aggtggtcgg taaaccagta gcgggcactg gacatcagcc ccagctggaa
    26161 catatagaaa tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct
    26221 aaagcgatct tcacgaatac ggtagccccg accaaaggca gaaagatctt ctgcttcaac
    26281 acgttgttgt tgcagttgct ctgattgacc aggcgctgta ccagccagct gtttgcgcag
    26341 gctggctacg tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct
    26401 aatcttctca acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc
    26461 ttcacgagag tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag
    26521 ctgaatttct ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac
    26581 cgtggtgtac tgcaatcctt cagattcagg cgcaggttga tatgccggtt ttggcgtatc
    26641 gcgcagggca gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt
    26701 gttaccgcgt tcataactca ggttgagatc tgcccagctg gcagcgcgat aaactgcacc
    26761 gacgttgaaa tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc
    26821 gtcgtattcg agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa
    26881 gatggaagcc gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc
    26941 tgcgcgatga caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt
    27001 accggcgtag ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc
    27061 caccagatac tcaccgtcaa acagacccgt accagcaata tcacgtttac caaacgccac
    27121 ttgcggtagc cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt
    27181 gtatgactga tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt
    27241 ataacgaatg gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta
    27301 ttgatcgtta tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg
    27361 catctgcaac aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta
    27421 ggttaatact tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata
    27481 agaattcttc ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt
    27541 aaatcagcat atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca
    27601 ggcggtggtt ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc
    27661 tcaggcgtaa tcaccataac gttattcttg tccgcgcctg caaggcgggg gtgatcttgc
    27721 agataatcgg taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca
    27781 cccagcagag taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac
    27841 gggggattgc tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga
    27901 ccagtgatat tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg
    27961 tcgtcagctt ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc
    28021 gctttagctt tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc
    28081 agttgcggtt gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt
    28141 tgttcgccag gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg
    28201 taaagtacgc tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg
    28261 tcatttctac gggaataacc cctgcgccta acatttgccg actttgacga acctggccag
    28321 tagccgaatc gacccagaat gtgttttgcc actgtttttc tggccgcgtg gaagtcacct
    28381 cttcctgcca gacattacac agcacattgc gacccgcaat attcagcgtc tctaatccag
    28441 caaaggaaaa agtagaactc agggtcgcag agcgaaaatg gttgtcttca ctccagagaa
    28501 tatcgcgcgt ccagcggctg ccatctttta ttgccagcgc gttacgcaga gggtcctgcc
    28561 cggaatttgt cacttccagc agattattat taagtttgac ggtttttaaa agacgtccat
    28621 tgtgggtaac cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat
    28681 atcccagtac cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag
    28741 gcaacgcctg gatctgctga tccgctacgg tgatatcctg attatcgaaa aggctggcgc
    28801 gaaatgtatc gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa
    28861 tcgataaaat aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc
    28921 tctaaagccc ggtacgaata tcgcaaccgg gtatcattta actttaattt cgacgcgtta
    28981 aagcagtatt agcgtgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca
    29041 ctgtcatttg ctgcaacaac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca
    29101 actgtcgccg tgccgacagc gccagcagcc acgccaccag tggtagtagc agtcgtggtt
    29161 gcgccggtag tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgtt
    29221 gcttcagggg cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa
    29281 agcttgtgtt tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc
    29341 aggattgtct actttgagta taacatcctt gtgaaatctc cctttcctca taccgcttgc
    29401 actatatact gcgggtagga aaggcgcgca gagggaaata taagattgtt aactatcccc
    29461 ctctggaaat acgacttgtg agcataagat aaatcttagc aaaatacaaa ttaaagataa
    29521 ggaaattgtt acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactataa
    29581 ctggctgtta aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag
    29641 tttctttgtg gaaaagtaac tgatattata atgtatggtg ttgactattg cactattcca
    29701 ataaaaccag tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct
    29761 gttggtcctc gcaggccatt tacgcggcaa aattccacac gtaatcctgg tataagcact
    29821 tctgcgtcgc ggggagtgaa tgcggaaata tggacctgaa cttctttacg accgtcggag
    29881 gggataatga atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta
    29941 cgggacaaac aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata
    30001 aagccagcga tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat
    30061 catgcgttaa tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag
    30121 cagttctcat tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtag
    30181 tttctgtatt aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta
    30241 ataaaatgac tggtttagta aaatggttta acgcagataa aggttttggc tttatcactc
    30301 ctgatgatgg cagcaaagac gttttcgtcc atttcactgc catccagagc aatgaattcc
    30361 gcacgctgaa cgaaaatcag aaagttgaat tttctattga gcaggggcaa cgtggccccg
    30421 cggcagcgaa cgttgttacg ctctaaggtt gccgttatta ctcaacatct ccatttccgc
    30481 tgtccatgtt gtcatggttc acagtaccgt acatcggcat tcgatgtgac ggagcgaaac
    30541 cctttgggcg ctaagtgtat tttttgtaaa tcgacgatga tcacctttga taacgtcgcg
    30601 ctgcaaatac gcacggacca tgcgccgctg gatttcacaa aataatatca ggctccctcg
    30661 tggagccttt tttatatctg ccttattttt cttcaacgct gtatgtatag taagcgataa
    30721 cctgttgatt attgaatctt tcggggagat ggcttataac atttcttacc tgaccagggt
    30781 accgggaacc aacaccttac tggcgtgttg ctgtctttca agaccagaag aggttaacag
    30841 tgaatattga agagttaaaa aaacaagccg aaacggaaat cgccgacttt atcgcgcaaa
    30901 aaatcgccga gctgaacaag aatacaggga aagaagtctc tgaaattcgc ttcaccgcac
    30961 gagaaaaaat gaccgggctt gaaagttatg atgtcaaaat caaaataatg tgattttgtg
    31021 aacatcaccc gtgcgaggtg atgttccgct tgttgctaat ttagtgacca atcattggcg
    31081 cttgtggaat taagcgtcgg tacaattcat ccggtaccgg gctttgccat actcccgcat
    31141 acattgcgta accaatcacc gcaaacataa tccccagaac cagtagcgtc attaaccagc
    31201 cagacaacgc aaaggctttt ttatttgccg caggtttttg cagtgaaaag gtcaatgttg
    31261 aggctaccgg acatgactct acgcaagtca tacagccggt acattccact gttcgtacct
    31321 gaattaattt atcgaccggg atccgtgatg ggcaattttt tgcgcatttg ccacagtcga
    31381 tacaactttc ggcattgcga cgaatcttaa acggcgacaa tagcgaaacc acgcccatca
    31441 gcgcgccata tgggcaaaga taacgacacc aggcatggcg aataaacagg ctggcaatca
    31501 gcaaaacggt cacgctgatt aatgtcgcgg tccccatatg acgaaagaaa tcgagcattt
    31561 taacgtccat caccacgctg tagggcgaca acataaaata gtgaatcgcc tgagcgggca
    31621 tcaataacgc gatatagaga aaaaaactca acagcaaata cttcacgccg cgcagaggaa
    31681 tatccagcca gcggggaagg acacattgcc gaccaaacag tttgttaccg agatcgccga
    31741 ttaattcaga aagcgtacca accgggcata accatgagca aaaggccttt ttgagtaata
    31801 gactgatgac gataaaagcg accaataaca gcatcgcggc ggcgtggacg gacggtaact
    31861 gacctgttac aaggctatat ttcagattca tcagcccggc aatcggtagc cagccttcga
    31921 tacctcccgg tctggcgaca aatgtcgtgc tacttgccgt ttcgtaatag cgcacccaat
    31981 accagaacgt gatggcaata taaatattca ttgccaacag taataattgc gtcgctttac
    32041 gccaggtcgt ggcattacgc cagtcattcc acggtaattt gccgcccgtc gtgcctggcc
    32101 gccgctgcca gcgggttctt ttattctctg ccatgatttt gccagtccgt taagttgtat
    32161 accaaatgcc actattctag ttgttcttaa ctggctgata ttgattcaaa tcgcgttcag
    32221 gtctttctta tgcaaccatg cttccagagc ggcaacactg cgtgtaattt cttcgtgtgg
    32281 aaggggggca gataatggct gctgctccag ttgtgcgcat agctggctgg cgatacgcat
    32341 tcccagactc gagcaactgc tttttagctg atgcgcggca cgctttattt tctcgctatc
    32401 ctgactggcg cgggcaatgt cgatttcatc gagaagcggc agggcatgtt gtgtaaataa
    32461 taccagccat tcgtggatct tctccgtccc cattaactga gcatcttcat tgagttgcga
    32521 tacatccagc gattgatcgt tattgacttg ccgttggaga tagtgcgcca gtaactgacc
    32581 gagcacttca cgcggcaccg gtttagggat aatcccgcgg aataatgaac tggtacgctg
    32641 gcgcagcgtt tcgtcaatga catgggcgct aaagccaatc aaaaccagcg acggatattg
    32701 ctgtgccagt tgtcgggcaa gcgtaatgcc gtcgacatcc ggcagatcaa aatccaccag
    32761 tgcggcagca aacggttcgc tattttgcag tgtctctaaa gcctgcgcgg cattgccaac
    32821 agcaacaacc tgcgcaccac tggtgttcag catctcaacg gtaattcgct gggttagcgg
    32881 gttatcttca attaacagca aacgtaaacc gtcaagacgc accgcctgat tgactgtttt
    32941 tggcacgggt gccgtggcaa cacgtaacgg caagcgtaaa caaaaacagc ttccaacctc
    33001 cggcgtgctg gtggcgctca gttcgccgcc catcgcctgg gccagacggc tactgatagt
    33061 cagccccagc ccggtgccgc cgcgtttgcc gcttacctgc acaaatggct ggaagatttc
    33121 tgccagtttc gcgggatcaa taccgcagcc gctgtcttcc acttcgacca gccattgctc
    33181 gccatcagtg cgactacgca ggataatgta cccttcgtca gtaaaacgca gggcgttgct
    33241 caacaggttg gttataacct gacgaatacg tcgtggatcg cccattaacg cgcacggcat
    33301 atcatcggca attgccgttg ccaggcgaat cgggcgacct ttcacccgtc cgctcattaa
    33361 ttgcagggta ctttccagca gcgggcgcgg ttcaaagggc tcatcgctga ccgaaacatt
    33421 cttgccacct gcttcgatag cggaataatc gagaatatcg ttgaggatgg tcagcaacga
    33481 ttcaccagag tcagtaattg cccgcaaatc atcacgctgg gcgttaagtg cggggttatc
    33541 tgccagcagt tgagcagtgc cgagaatacc gtacagcggt gtgcggatct catggctcat
    33601 cgccgccaga aacgccgatt ttgcctggct ggctttttct gcttccgccc gtgcctgtcg
    33661 gtgttctatc accagttcct gcaattcagc tgtacgcgct ttgacctgcg ccgccagctg
    33721 ttcgcggtgg cgattcagtg catgaacatt gctgcgaaac gcatccatca gccgcccgat
    33781 ggtatccagc tcccgtacgc cagcggtttc cgggaaaggg gagtcaatat caccgtccag
    33841 cagccgttgc agcgcctgcg tttgttcggc aagtggacgc gtgactgagc gataaaccac
    33901 gcgccaaagg atcagaatca gtgcgcaaag tgaaaccatc cccagcaata acaggctgta
    33961 ttgcccgcgt gcactggctt tttccagatg cgccagtccg tgctgattac gcagctcaat
    34021 ggtgtcgacc agctgactga cttcgctact aaactgcgcg aactgggcga tgttattttg
    34081 tgcgagagtt tgtaggtgat tgctgatttc actgtcctgc tgatacagcg ccagcaaatc
    34141 gctatattgg ctaacggtag ttaacgttgt tgcgacctgc gcacgaacac ccggatcttc
    34201 aatgcgtatt tgccgacgtt gcagaatttt caccgcatta ttgagctgct tttccagcgt
    34261 tggtgcattt ttctggatct gctccagccc cagattcatc accatttgct gcacccgcag
    34321 agcgctaagg cgcagttcat tcatctggtt aacatactca agatcgatat caatcagccg
    34381 atcgagtgca ctttcagcag cctgacgctg atcttgttcg atcaaatcgt aaatcccggc
    34441 ctgggtcgct ccagcggaag ttgtcgcatt attcgcctga ccttgcgcca ggcgtgcgat
    34501 ctcatcggcg gcagcgacta tctgctgact gagttgccgt tgttgctggc gtagttgcag
    34561 acgccgcccc accagttccc cttgctgacg taatgaacgg gagatctcct gctcctgttg
    34621 ttcaatagcg gtggtatcaa aaccttgttc ccgtaacgct tgcagcaacg cattaatctt
    34681 caggctttgt gcggtgagca ttcgcccctg cgcctgccac atcttttcgt tatcggcact
    34741 ggtcaggttc tgcgcggcga aaagttccca ggcgctggct tcgctcaact ggcgcgccat
    34801 attcatggta ggaatcaatg cctgagtgtt gtctttttcc acctggctga taaagcgcag
    34861 gttgtaccat cccaccaggg tactggtcag ggttaacagc gccatcaggg caaagcccat
    34921 ccagagtctt cgggtcaggg ttaaattcac ggtcggtgca cttaaggtga aaaaggttga
    34981 gtcgcaaagc ggaatgcatc tagcataaag ccttattatt gatgaggcta tcatgcgcgt
    35041 actgctattt ttacttcttt cccttttcat gttgccggca ttttcggctg ataacctgtt
    35101 gcgctggcat gatgcgcagc atttcacggt gcaagcctct atgccgctta aagccaaacg
    35161 cgcatggaaa ctgtgcgcgc tttatcccag cctgaaagat tcatactggt tatcgttgaa
    35221 ctatggtatg caggaggcag ctcgccgcta tggtgtggat ttaaaagtgc tggaggcagg
    35281 cggctacagc cagtttgcta cccagcaagc acaaatcgac cagtgtaaac agtggggcgc
    35341 agaggccatt ttactcggta gtagcacgac ctcatttccc gatctgcaaa agcaggtagc
    35401 aagtctgccg gtgatcgaac tggtaaatgc tattgatgct ccccaggtga aaagccgcgt
    35461 tggtgtaccc tggtttcaga tgggctatca acctgggcga tatttggtgc aatgggcgca
    35521 cggtaaacca ctgaacgtgc tgttgatgcc cggacccgat aatgccgggg gcagtaaaga
    35581 gatggtcgag ggttttcgcg cagccattgc cggaagcccg gtgcgtattg ttgatattgc
    35641 gcttggtgat aacgatattg aaatccagcg taacctgttg caggagatgc tggaacgcca
    35701 cccagaaatc gacgtcgttg ccggaacggc cattgcggca gaggcggcaa tgggtgaagg
    35761 gcgtaacctg aaaacgccgc ttaccgtggt gtcgttttat ctttcacatc aggtgtatcg
    35821 cgggctgaag cggggaagag tgattatggc tgccagcgat caaatggtct ggcaggggga
    35881 actggcagtt gagcaggcca tcaggcaatt acaggggcaa tcggtgtctg ataatgtcag
    35941 cccaccgatt ttagttctga cgccgaaaaa tgccgaccga gaacatatcc gccgctcgct
    36001 gtcaccaggg ggatttcgtc cggtctattt ttatcagcac acatcagcgg ctaagaaata
    36061 accttcacca tgttgcgtca ccagtaaatc cgcgctgagt ttatgacgta aacgacgaat
    36121 taacacatcg acggtgcgca ggtcagggtt ttccacccga cgcgcagaaa gcatacgtag
    36181 cagacgttca cggctgagaa tttcgcccgg attcgtcaca aatgccacca acatttcata
    36241 ctctgcgcgg gtcagtttaa tcggctcgcc atcccgctcc agcgtatggc gcgacacatt
    36301 caggcaataa ccggcaaagc gatagcagtt gtcctgagtg tgcggttgag cttgtcgcgc
    36361 gaggtcgatt cgccagagca gatttttcac ccgtactacc agttcgcgca gttccagcgg
    36421 tttggtgacg taatcgtctg cgcccatttc cagcccaaca atacggtcaa tccgatcgct
    36481 gcgtccggta accagaataa tccccaccgt tgagcgttct cgcagggcgc gggttaacat
    36541 caggccattt tcatcgggta agttgatatc cagcagaatt aaatctaccg gctgattctg
    36601 cataatttcc cgcagcccgg caccgctcgc tgtaacggaa acggtatacc cctcctgagt
    36661 gaagtaagat tgtaatcgcg cctgggtaac cggctcatct tcaacaataa caatgtgatg
    36721 tggcatcaga gggttttact cattctgttc atatctgttc atattctgcc gtaagccgtt
    36781 catcctgacc agtgccgctg ttcatatttg ctcattaaga tcgcttcact aaaccataat
    36841 tttacagggg ctattatgcg gaaactctgg aacgcgctac gccgacccag tgctcgttgg
    36901 tcggtactgg cgctggtcgc aattgggatt gtgattggca ttgcgctgat tgtattgcca
    36961 cacgttggga tcaaagtcac cagcacaacc gaattttgtg tcagttgcca cagtatgcaa
    37021 ccggtgtatg aagaatataa acagtcggtg catttccaga acgcctccgg cgtgcgagct
    37081 gaatgccatg actgtcatat cccgccggat attccaggca tggtgaagcg caaactggaa
    37141 gcgagcaatg atatctacca gacctttatt gctcactcca ttgatacacc tgaaaaattc
    37201 gaagccaaac gcgcggaact tgccgagcgt gaatgggcgc gaatgaaaga aaacaactcg
    37261 gcaacctgcc gctcctgcca taactacgat gcgatggatc atgcgaagca gcatcctgaa
    37321 gcggcacgtc agatgaaggt ggcagcgaaa gataatcaat cctgcatcga ctgtcataaa
    37381 ggtattgccc accagttacc ggatatgagt agcggcttcc gtaagcagtt cgatgagctg
    37441 cgcgccagtg ctaatgacag tggtgacacg ctgtactcta ttgatattaa gccaatttat
    37501 gcggcgaaag gcgataaaga agcctctggt tctctgctgc ctgcttcgga agtgaaagtc
    37561 cttaaacgtg acggcgactg gctgcaaatt gaaattaccg gctggacgga aagcgccgga
    37621 cgtcagcgtg tactcaccca attcccaggt aaacgcatct ttgttgcctc gattcgtggt
    37681 gatgtgcagc agcaggtaaa aacgctggag aaaaccaccg ttgccgacac caataccgag
    37741 tggagcaagt tgcaggccac tgcgtggatg aagaaaggcg acatggtgaa cgatatcaaa
    37801 ccgatctggg cttatgcgga ttcgttgtac aacggcacct gtaaccagtg ccacggcgca
    37861 ccggaaatcg cccactttga cgctaacggt tggatcggca cgctcaacgg catgattggc
    37921 tttaccagtc tcgataaacg tgaagaacgc accttgttga aatatctgca aatgaatgcg
    37981 tctgacaccg caggtaaggc tcacggcgat aagaaggaag aaaaataatg aacaataacg
    38041 atctctttca ggcatcacgt cggcgttttc tggcacaact cggcggctta accgtcgccg
    38101 ggatgctggg gccgtcattg ttaacgccgc gccgtgcgac tgcggcgcaa gcggcgactg
    38161 acgctgtcat ctcgaaagag ggcattctta ccgggtcgca ctggggggct atccgcgcga
    38221 cggtgaagga tggtcgcttt gtggcggcaa aaccgttcga actggataaa tatccgtcga
    38281 aaatgattgc cggattgccg gatcacgtac acaacgcggc gcgtattcgt tatccgatgg
    38341 tacgcgtgga ctggctgcgt aagcgccatc tcagcgatac ctcccagcgc ggtgataacc
    38401 gttttgtgcg cgtgagctgg gatgaagccc tcgacatgtt ctatgaagaa ctggaacgcg
    38461 tgcagaaaac tcacgggccg agtgccttgc tgaccgccag tggttggcaa tcgacgggga
    38521 tgttccataa cgcttcgggg atgctggcga aagctattgc cttgcatggt aatagcgttg
    38581 gtacgggcgg agattactct accggtgctg cgcaggtgat cctgccgcgc gtggttggct
    38641 caatggaagt gtatgaacag caaacctcct ggccgctggt attgcagaac agcaaaacca
    38701 ttgtgctgtg gggctctgat ttgctgaaaa accagcaagc gaactggtgg tgcccggatc
    38761 acgatgttta tgaatattac gcgcagctaa aagcgaaagt cgccgccggt gaaattgagg
    38821 tcatcagcat cgatccggtt gtcacatcca cccatgagta tctggggcgc gagcatgtga
    38881 agcacattgc ggttaacccg caaactgatg tgccgctgca actggcgctg gcgtatacgc
    38941 tgtacagtga aaacctgtac gacaaaaact tcctcgctaa ctactgtgtg ggttttgagc
    39001 agttcctgcc gtacctgctg ggtgagaaag acggtcagcc gaaagatgcc gcatgggctg
    39061 aaaaactgac cggcattgat gccgaaacca ttcgtgggct ggcgcggcag atggcggcga
    39121 acagaacgca aattattgct ggctggtgcg tgcagcgtat gcagcacggt gaacagtggg
    39181 cgtggatgat tgtggttctg gcggcgatgc tggggcaaat tggcctgcca ggtggtggct
    39241 ttggttttgg ctggcactat aacggcgcag gcacgccggg gcgtaaaggc gttattctga
    39301 gtggtttctc cggctctacg tcgattccgc ctgttcacga caacagtgat tacaaaggct
    39361 acagcagcac cattccgatt gcccgtttta tcgatgcgat cctcgaaccg gggaaagtga
    39421 tcaactggaa cggtaaatcg gtaaaactgc cgccgctgaa aatgtgtatt tttgccggaa
    39481 ctaacccatt ccatcgccat cagcagatca accgcattat tgaaggctgg cgcaagctgg
    39541 aaacggttat cgccatagat aaccagtgga cctcaacctg ccgctttgcc gatatcgtgc
    39601 tgcctgcgac cacgcagttt gagcgtaacg atctcgacca gtacggcaac cactccaacc
    39661 gtggcattat cgccatgaaa caggtggtgc cgccgcagtt cgaggcgcgc aacgactttg
    39721 atattttccg cgagctgtgc cgtcgcttta atcgcgaaga agcctttacc gaagggctgg
    39781 acgaaatggg ctggctgaaa cgcatctggc aggaaggtgt acaacaaggc aaaggacgcg
    39841 gcgttcatct gccagcgttt gatgacttct ggaataacaa agagtacgtc gagtttgacc
    39901 atccgcagat gtttgttcgc caccaggcat tccgcgaaga tccggatctc gaaccgctgg
    39961 gcacgccgag tggcctgatt gagatctact cgaaaaccat cgccgatatg aactacgacg
    40021 attgtcaggg gcatccgatg tggtttgaga aaatcgaacg ctcccacggt gggcccggct
    40081 cgcagaagta tccgttgcat ctgcaatctg tgcatccgga tttccgactt cactcgcagt
    40141 tatgtgagtc ggaaactctg cgtcagcaat atacggtagc gggtaaagag ccagtgttca
    40201 ttaacccgca ggatgccagc gcgcgcggta ttcgtaacgg tgatgtggta cgcgtcttta
    40261 acgctcgcgg tcaggtgttg gcaggggcag tagtttctga ccgctatgca cccggcgtgg
    40321 cacgaattca cgaaggggca tggtacgatc cagataaagg cggcgagcct ggtgcgctgt
    40381 gcaaatacgg taaccccaac gtgctgacca tcgacatcgg tacttcgcag ctggcgcagg
    40441 cgaccagtgc gcacactacg ctggtggaaa ttgagaagta caatggagca gtggagcagg
    40501 tgacggcgtt taacggccct gtggagatgg tggcgcagtg cgaatatgtt cccgcgtcgc
    40561 aggtgaaatc atgaccacgc tgacagcaca acagattgcc tgtgtttacg cctggctggc
    40621 gcagttgttc tcccgtgaac tggacgatga acaactgacg caaatcgcca gtgcgcagat
    40681 ggctgaatgg ttttcgttgc tgaaaagcga accgccgctg gctgcggcgg tgaatgagct
    40741 ggaaaactgt attgccacgc tgacagtacg tgacgatgcc cgtctggaac tggccgctga
    40801 cttttgcggc ctgtttctga tgaccgacaa acaagcggcg ctgccgtatg catcggccta
    40861 caaacaggac gagcaagaga ttaaacgctt gttagttgag gcagggatgg aaaccagcgg
    40921 caatttcaac gaaccggcag atcatctggc gatctatctc gaattgctca gccatctgca
    40981 tttttcgctg ggagagggga ccgttcctgc gcgaagaatc gacagtttgc ggcaaaaaac
    41041 actgacggcg ctgtggcaat ggttaccaga gtttgttgtg cgttgtcgtc agtatgacag
    41101 ctttggtttt tacgcggcac taagccagtt attgctggtg ttagtggaga gcgaccacca
    41161 aaacagataa cgtcgtttgt gcgcctgaaa agacgcgttt agcgtcgcat caggcattat
    41221 ggggcagttg ccggatgcgt catcacatcc ggcaaacaca catcacggat gagctacaaa
    41281 ccgggaaagt cgctggcgca gcaggcggtt ttcctgcttc aggtgcgcaa tatcatccat
    41341 taacgtcagc gccaccgcga tcccaggcca gtccagagcc agttcatgac gcaggcgtac
    41401 tgcgcgttgc accacgatgg cggcatggtc gtcaaatacc caggttgttt cctggatctc
    41461 acgcggttca accaccccca aaccgacaat ttcattcaac tcctcttcag agatgccggt
    41521 atgcaggcaa aattcggtaa tagtaaaagt caccgtaaca ttagccatta tgctttcccc
    41581 caatctttac gtggatcaaa agacgactgg gcgtctgcca gttgctgcca cagcgcggca
    41641 gtgttttcat ccggtttcgg cggcatcacg attttcagta ccgcatacag atcgccggtc
    41701 tgttttttgc tcaccagacc tttgccttta acgcgcaatc gttgcccggc ctggctgcct
    41761 ggcgggatag tcagcaaaat gctttctttc agtgttggaa cggtgacttt agcacccagc
    41821 gccgcttccc acgggctaac cggcaccaca atttccagat cctggccgac aatatcgaac
    41881 agcggatgtg gcgcaatatg aatcaccagc cacaaatcgc catttggacc accgttttcg
    41941 cccggcgtcc cctggccttt cagacggatg cgttgaccat tgccgacgcc cgccgggatc
    42001 ttcacattca gcgttttcgg gatttcctgt tcgatcatgc caaaggcgtt ataaaccggc
    42061 aggttatagc tgatggtacg cttatgctca gtcagcgttt cttcgaggaa taccgccact
    42121 tcgatttcaa tatcgtggcc gcgtgcggcg gggcgttgac ggctctggcg ggcatgctga
    42181 ccgaaaattg acgagaagat atcgtcaaaa tcttcggcat taaaactctg accgtcgcca
    42241 tgatggaact gacggttaaa ttgcggatcg ttgcgatgtt gccacatctg atcatactca
    42301 gcgcgacgtt gttcatcact taacacttcc caggcttcag cgacctcttt gaagcgggct
    42361 tcggcatccg gttctttgct gacatcagga tggtatttgc gggccagtcg acgataggcg
    42421 gtcttgattg tcttgagatc gtccgtcggt ttcacgccca tgatggcgta ataatccttt
    42481 aattccatag cgttatctcg cgtaaatcaa cacaaattga aggaacccct gtaaggtaac
    42541 tcctataagt gtagggtaat cctcaaaatt tcatatgcca acacagaata tgttattgaa
    42601 atcatcgcgg agaggaggtc gcctcaaggt ggggtgctga acatatttta aacaggtgaa
    42661 aaagggtgag cgatttttga tagtggaacc aggcacttta agtttaactg gggcgtcatt
    42721 atttattaaa ttttataggc gctatatatg agtagtaata tacatggaat tagttgcact
    42781 gcaaataatt atttgaaaca agcctggaac aatataaaaa atgagcacga aaaaaatcaa
    42841 aaatattcaa tcacgctttt tgaaaacaca ctggtgtgtt ttatgcggtt gtataaagaa
    42901 atcagacgtc aaaaagctga agactacatt ccatgcctgg aatgcgattc actagaaaaa
    42961 gaatttgagg aaatgcagaa tgataatgat ctatcattat ttatgagaac attgcgtact
    43021 aatgatacac aaatttattc aggggtttca ggaggtatta catatactat acaatatgtt
    43081 caaaatgttg atattgttag agtgtccttg ccgggcagag gttcagagtc tatcacagat
    43141 tttaaaggtt attattggta tggctttatg gaatatattg aaaacattac tgcgtgtgat
    43201 gatgtttttt ctgagtattg tttagatgat gaaaatatga gtatccagcc agaacagata
    43261 aatacgccgg gaatatctga tttagattct ggtattgatt tgtctggtat atcttttatt
    43321 cagagtgaaa ttaataaaac ttatggatta aaatacgctc cagtagatgg cgatggctat
    43381 tgtctgttaa gggccatact ggttttaaaa gaacatgagt attcatgggc gctgggcagc
    43441 cataagacgc aaaagcaagt atacgaagag tttattaaaa ttgttgataa acaaacgatc
    43501 gaggctcttg ttgatacggc attctataat ctcagggaag atgtaaagac gttatttggc
    43561 gttgatctac aatctgacaa caaaattcag ggggagggta gtcttatgtc atggaacttt
    43621 ctgtttttta aaaaataatt tattgatagt tgcttgaatg acaaaaaatg catactgcat
    43681 ttacccgagt ttatatttaa tgataacaag gcacgccttg ttttagacac cgacccagag
    43741 aagaggatta atgaggtgaa aaatttcctc actgctcttt cagatagcat ttgctcatta
    43801 tttattgtta atagtaatgt ggcatcaatc tccttaggga atgaatcctt ttcaacagat
    43861 gaagatcttg agtatggtta tttaataaac actggcaatc attatgatgt ttacctccct
    43921 cctgaacttt ttgctcaggc ttacaagtta aaaaataagg aaatgaatgc gcaactcgac
    43981 tatttaaatc gttatgcaat ttaatggcaa aggcatatgc taaaaaccat tgttattagt
    44041 ctcacacttt tttattggta aatattgtct ctgtattggt aacgccgcag atattctgtt
    44101 tagccacagg tgcaattatc agcggcgtac gcgaggcagg ggctaatcag gcatagtttg
    44161 cgtcaaacct tgcctgtttt tgaagatgta tatagaaaaa caggcgttca acaagccatt
    44221 ttgcgaacct gttcccggaa aaaagtcata tttctgtcac actctttagt gattgataac
    44281 aaaagaggtg ccaggaatga acaaaacgct aatcgccgca gctgtggcag ggatagtttt
    44341 actcgcttca aacgctcagg cacaaaccgt accggaaggc tatcagctac agcaagtgct
    44401 catgatgagc cgccataact tacgtgcgcc gctggcgaac aatggcagtg tgctggagca
    44461 gtcgacgccg aataaatggc cagaatggga cgtccccggt gggcaactca ccaccaaagg
    44521 tggcgtgctc gaagtgtata tgggccatta catgcgtgaa tggctggcag agcaggggat
    44581 ggtgaaatcg ggggaatgcc cgccgccgta caccgtttat gcctatgcca atagtctgca
    44641 acgtaccgtt gcgaccgcac agttctttat taccggcgca ttcccggggt gtgatattcc
    44701 tgtgcatcac caggaaaaaa tgggcactat ggacccaacc tttaacccgg tgatcaccga
    44761 tgattccgcc gcattcagtg aacaggcggt ggcggcaatg gagaaagagc tcagcaaact
    44821 ccagcttacc gacagctacc agctactgga aaaaatcgtt aactataaag attcccctgc
    44881 ctgtaaagag aaacaacagt gttcgctggt ggatggcaaa aataccttta gcgccaagta
    44941 tcaacaagaa ccaggtgttt ccgggccgct gaaagtcggc aactcgctgg tagatgcgtt
    45001 tactttgcaa tattacgaag gttttccgat ggatcaggtg gcctggggag aaatcaaatc
    45061 tgaccagcag tggaaggtgt tgtcgaagct taaaaacggc taccaggaca gcctgtttac
    45121 ctcaccggaa gtggcgcgca atgttgcgaa accgctggtc agttatatcg acaaagctct
    45181 ggtcaccgat cgcaccagcg caccgaaaat tacagtgttg gttgggcacg actccaacat
    45241 tgcctctctg ttaacggcgc tggatttcaa accgtatcag ttgcatgacc agaacgaacg
    45301 cacgccgatt ggcggcaaaa tcgttttcca gcgttggcat gacagcaaag ccaatcgcga
    45361 tttgatgaaa attgaatatg tgtatcagag tgcggaacag ttacgtaatg ccgatgcgtt
    45421 aaccctgcag gcacctgctc agcgtgtgac gctggaatta agcggttgcc cgatagatgc
    45481 caacggtttc tgcccgatgg ataaatttga tagcgtgttg aatgaagcgg tgaaataaca
    45541 gaaaactccc ccgcgagaag cgggggagtc gctggttaaa cgtttttacg ttcgatggtc
    45601 tgttcgcccc aaaaaagcga atctttatcg gtcttagcaa aggctttgac taacacttca
    45661 tcgctacctt cttcccaaat cttttccgcc attttttcgt cgtacccggc gacttcgaaa
    45721 atggcctcgg ctatttccgg cgacgtattg cgcagagatg cccattcacc gacgtgatga
    45781 gctttcgctt cttgagttgg catgcgtatc ctcctgttga agattagccg ttaagtttaa
    45841 ctgccagacc tgcgacatat tccccttgat aacgagcaat agacagttct tcctggcttg
    45901 gctggcgtga gccgtcaccg cctgcgatgg tggttgcgcc gtacggcgta ccgccgcgaa
    45961 cctgtgaaac gtcaaataat tcctgcgctg cgtagccaat ggggacaatt accatgccgt
    46021 gatgcgcaag ggtcgtccag gttgaagtaa tagtttgttc ctgaccgccg ccagtaccgg
    46081 tggaactaaa gacgctcgcc agttttccgt atagtgcgcc ggaagcccac aggccgcccg
    46141 tctggtcgag gaaggtacgc atttgaccgg acatgttgcc aaagcgggta ggtgtaccaa
    46201 aaataatggc gtcgtaatcg gccagttctt gcggggttgc aaccggtgca gtttgcgttt
    46261 taccgcctgc tttttcaaat aattgcggcg gcatggtttc cggtacacgc ttaacgacaa
    46321 cttctgcgcc atcgactttg cttgcaccct cagcgactgc gcgtgccatc gtttcaatat
    46381 gtccgtacat ggaataataa agcaccagaa ctttagccat ttctaaccac tcctcgtgtt
    46441 atctctattc cgtagcgatt cgctaccact tatttaaaga taagacgtcc ttttcagagt
    46501 gcaaatttca caaccactta tttgatttat aacaactttc acaagcacgt aattttgtcg
    46561 caaaatgaca catttttatc tcatcgcgtt tttttaatca taagagcggc ttatggataa
    46621 ttatttgaga tgatatctat tctcgctaag aagctgttgc aggatattac caaacgcggg
    46681 tctgcccgcg tcagttcact aagcttagtc ccacgtagcg aaaatatggc agccgccata
    46741 cgccgcgtta attctatgca atatgatgtc tatacccaga cggaggtcag taatggcaaa
    46801 ccatcgaggc ggttccggca attttgcaga agaccgcgaa agagcatcag aagcaggtaa
    46861 aaaaggtgga cagcacagcg ggggtaattt caagaatgac ccgcagcgcg catctgaagc
    46921 aggtaaaaaa ggtggcaaga gcagtcacgg caaaagcgac aactagccgg gctaatcaat
    46981 gacgaatgca tttttttctt aggctcgtca aaaagtcatc accgccggta atcccggcgg
    47041 ttttctgttt atggctcctg atgaacaact tctggcggtg gaacgtcaac caactttctg
    47101 cttaacaacg cattgagtaa aatcgcacca aaggttgctg taccaatccc tcccaacgta
    47161 aaaccgccca gcgtgagagc aaaatcaccc gcgcccagca ctaaggttac tgcgaccata
    47221 atcaaattac cgttctggct taaatcgaca cggttttgta cccatatcct tgcgcctgcg
    47281 acggcaatca gcccgaacac aacaattgat gcaccaccaa tcaccgcggc cggaatggta
    47341 tgaatcagcg cgccaaattt cggtgaaaag cccaacagca tggcgatgac ggcagcagcg
    47401 acaaacacca gcgtcgagta gacttttgtc acggccatta caccgatatt ctcggcgtag
    47461 gtggttacgc cgctgccacc gacagaacca gaaagcatcg tcgccagccc atcaccgacg
    47521 aatgcccgcc ccatatacgg gtccatattg cgtccggtca tcccggcgac cgccttgaga
    47581 tgacctaagt tttccgccac cagaatcacc gccacgggcg caatcagcat cattgcctga
    47641 ctattaaaag caggagtgga aaaatgtggc aaaccgaacc aggcagcatg gctgacgaga
    47701 gtaaaatcga cggcttttcc cagccctaaa acgttggtca tcacgccata cagcagacag
    47761 gcgacaatta aacctacgag aatcaataac cgctggatca tgccacgggt aaacaccgcc
    47821 accagcccaa tacacagcac cgtcattacc gccatccagc tatcaaaggc cgaagccgat
    47881 acacttttca ctgcgatagc gctaagttca ggccaatcgc catcaccacc gcacccgtca
    47941 ccaccggcgg cattagtcgt tcaatccagc gtgtaccaat tttcatcacc accaggccaa
    48001 tgacggtata aaccagccca caggcgataa tcccgcccag cgcaatgctg atattcgggt
    48061 taatgccctg accgttaaag cccgtcgcgg caatcaccac gccgacaaaa gccgcgctgg
    48121 agccgagata actggggacg cgcccgccgg taataaagaa aaacagtaac gtgccgatcc
    48181 ccgacattaa aatggaaaga ttgggatcca gccccatcag aatcggcatt aacaccgtcg
    48241 cgccaaacat cgccaccgcg tgttgaacgc ccattattgc cgtctgagca aacggcaatc
    48301 gttcatccgg cgcgaccacg ccgctctctg tagaggtcga ttttaactgc cagtgaggaa
    48361 aaccgaacat tgccatcagc tgtctcctta aggaggttaa caagcagggc gcatcagcgc
    48421 gtgataactg cgatcgaacc acaccagccc gtagggtgtg gcgtgacgat gaatagcttc
    48481 gatggcgcaa aacaaaatgt cgtgggtgcc gacgctcacc acctggctga tacggcagtc
    48541 aaacgaaacc agtgcctctt ccagttgcgg gcatccggtc acacctgtct gccagcgggc
    48601 ggcggcaaag cggtgttcca tgggcgtttt gccgccaaaa aggttggaaa gcggctcctg
    48661 cccggcgcta agtgtattta cacacagcgt tcgattttca ttgaataccg gccagacgga
    48721 cgccccacga ttcaggcaca ccagtaatgt gggcggcgta tcggtcacgc tgcagacggc
    48781 gctggcggtg aacccggcgc gcccggctgg accgtccgtg gtgatgatat tgaccgccgc
    48841 gcccatgcag gacatcgcat cgcgaaaagt ttgttgatcg acaatgttca tagtttgctc
    48901 cttacaacag cccgcaggct tcttcaaagg acagacgtgg caggcgcgca aaaagtttgc
    48961 ttctatcgcc atagccgata ttaatcagca gattgctctt cagcgtgctg cccgcaaaaa
    49021 aggcgtcgtc aacgtgttga cggtcaaaac ccgacatcgg gccggtatcc agtcccagcg
    49081 cacggcaggc gacgatcaga taggccgcct gcatggaact gttgcgaaac gctgtttctt
    49141 ccgcaagttg tgggctggag gtaaaccaac tgcgggcatc gccgtgagga aacagttgtg
    49201 gtaaccgttc ataaaattca ctgtcccagg caacgatagc ggtgacgggc gcggtcaggg
    49261 ttttttgtag attgccgctg gaaagcgccg ggcgcagacg ttcttttcct tctgcagtgc
    49321 ggataaacac gatccgtgcc ggagaacagt tagctgatgt cggcccccat ttcatcaggg
    49381 cataaagctc ccgtaacgtc tcatcgctga cgggtgtctc ccgccagccg ttgtgagtgc
    49441 gggcatcggt gaacagggtg ctaagcgcac ctgggctaac ggcttcgttc atagcaattc
    49501 cttacagggc ggcttcacgg tgatgtaaca ggctggcaag cccgttgagt aacagagcat
    49561 taaacgtttc gggatcggtc acgttgcagg cgtgtccgcc atagcgcatc accattttct
    49621 ggctatcggg cagggcggca tgaagttcac tggaacatgc ggatggcacc agcagatcat
    49681 cactggcgca gatgatttgc accgggcagc ggatgcgatc cgcatggtga ctaaagtcag
    49741 cgcgtttgag ggcgttaagt cgacgcagta aattattttt gccctgaaaa tgcgccagtg
    49801 ccagcgcgtc ttctgcctcc aggcgaggtg cgcgggccgc catccagtcg gcgggataga
    49861 ggaacaacgg ctgcgcttcc acccatgcct gtgcgccgcc gctatacagt aatcgttcgc
    49921 gaacctgaaa acagcggcgc gtatgggcgt ttattcgtag ccagccgtta acgctgacca
    49981 gcatagttac cgacgcggga taatccagcg ccagctgcat ccccaccagc gcaccgagcg
    50041 catggccgac caccgcgtaa cgctcaatcc ctgcggctac cagcgcctga tgcagttccg
    50101 ctgccatctg ggtgatactg taatcttctg ccagcgtgtc gggattattg ccggtgccgc
    50161 gctggtcgta acagactacc tgatactcct gctccagcac cgccagttgc ggtaaccagt
    50221 aactgccgct accgccaaga cccgaaatca acaccactac gggtgcatca gcataagggg
    50281 gaggtgagag tgatagtttc atcgcggcct cacttggcga tatgcgcaat tgtggcgatt
    50341 tccaccagcg cgtcaggttt taccagtccg cactgaatgc agaatcgcgc cggtttatca
    50401 cccggaaaaa actcggcgta gatttcgtta atcgcggcgt aatttttcca gtcggtaata
    50461 aagatgctgt tgaaggtcac atccgccatc gtgccacccg ccgtctcgat caccttgcgg
    50521 atagtttcca gaacgtggcg ggtttgcgcc tttgggtcat cggcaaacag cacgttatta
    50581 tgttgatcaa aagccagcgt accggagaca tacaccacgc catcagccag cgtgccggga
    50641 acgaaggggg ccagcggtgc gctgctgcca gcgggaataa ttacggattt tggcatcgtt
    50701 aaactcctta agcgatacga gcaaaggacg tgggagaaag cgcgtcgcag aacgtttcga
    50761 cgtcgctgac ccagccaaaa aaggtttcga tattgaacaa cgcggctttc tgtacaaatt
    50821 ccggccccgc ctggtgtgtt gcgtcttcca gcaccacgcc gaaatactcc agaaaaaagc
    50881 catcgcgtag cgtcgattcg acgcagacgt tggtagcgat accggtgaaa accagatggc
    50941 gtattccacg gctgcgcaaa atgctgtcca gcggcgtatt gaagaaaccg ctgtagcgcg
    51001 gcttcggcag cacaatatcg ccaggctgcg gcaccagttc atccaccagt tgataatccc
    51061 aggagccttt cgccagcaat ttcccctgca gctgcggctg cttacgcatg gttttcaggg
    51121 cgttcgattt atgaaaattc ggtgagccgg gtccgcctgc ttcgacatac tgttcatccc
    51181 agccattttg aaaccagatg atcagcatcc ctgccgctcg cgctgcggtc acggcggttt
    51241 gaatgttggc aatgaccggg cgagtggttg agacatcaaa cccggcgaga tctaagtagc
    51301 ctcctggcgt ggcataagcg ttttgcatat ccaccacgat cagcgcactt tgctgcggat
    51361 cgaaggtaat ggcttccggt cgagcggtta aggtcgtcat catgccacct cctgagtcag
    51421 cgcagggaga tgggcgcggc actgcatcag tggttgaatg cgctcgccga aggtttcgat
    51481 tcccgacaga aaatcatcga aggtcaacag cacgccttcg gcaccaggca cgcttgcgac
    51541 ttcatctaac atgcgcgcga cactggcgta agaaccgact aacgtcccca tattgatgtt
    51601 taccgccgaa gtgggatcgg ccatctgacg gacgttggtg tcagtaccgg agcgggtatc
    51661 tttctgactt tgttcggtta gccagcttaa cgcctcttca tccgcgcccg ctttgtagtg
    51721 ttcccatttg gcgcgagcgg catcgtcggt ttcatcggca atcaccataa acaatacata
    51781 agagccaacg tcgcgcccgg tttgctctgc ggcctgtttc atccgcgcag cggtcggggc
    51841 gaaagccgtg ggtgtattta cgcctttgcc gaaacagaag ttgaaatcgg catactgggc
    51901 ggagaacgcc atgccagcgt cgctttgccc ggcgcagatc actttcatgg gtacactcgg
    51961 ttgcggactg acgcgacaat cattcatggt gaaaaaatcg cctttaaaat cgcttttccc
    52021 cgagccccac aggtcgcgca gcacctgaac atattcggtg agatagtcgt aacgacggga
    52081 gaaatagtca tcgccaggcc agatacccat ctgctcatac tcgggctttt gccagccagt
    52141 cacgaggttg acgccaaaac gcccgccaga gatggagtca atggttgcgg ccatacgggc
    52201 gacgattgcc ggaggtaacg ttaaggtggc agctgtcgcg taaatctgaa tgcgtgaggt
    52261 gacggctgcc agccccgcca tcaaggtgaa cgactcaagg ttgtgatccc agaactcagt
    52321 tttgccgcca aagccacgca gtttgatcat cgacagggcg aaatcgaaat ggtagtgctc
    52381 cgctttttgc acaatggctt tattcagttc aaaggtcggc atgtactgcg gcgcgtgggt
    52441 cgaaatgagc cagccgttgt tgccaatagg tacgaatacg ccaattttca tcatcaacct
    52501 ctcttcgtct cgtaaagtga aagtcagacg gggcactgcg tcctgcatat ccttttcagc
    52561 cgcgtaatgg cttgtttgca aagtggatgc cagtttttaa aaagtgaatg ttattaatgt
    52621 gttaatgcta agttatctaa aatatctggt aaaaagtgga ctaaacggtc aaaacagttg
    52681 cacataaaac atgcatccgt gcgcgatgag agtgcagaag gtcgaggccg gacgggggtt
    52741 ttgctatcct gttgccaatc tacaagaggg gagagcgcat gacgcaaggc gcagtcaaaa
    52801 caacgggtaa acgttcgcgt acagtaagcg cgaagaaaaa agcgattctt agcgcagcgc
    52861 tggacacttt ttcacaattc ggttttcacg gcacaaggct ggagcagatc gcagagttgg
    52921 cgggtgtttc aaaaaccaat ctgctgtatt actttccgtc aaaagaggcg ctgtatattg
    52981 ccgtgctgcg gcagattctc gatatctggc tggcaccgtt aaaagcgttt cgtgaagatt
    53041 tcgccccgct ggcggcgatc aaagagtaca tccgtctgaa gctggaagtc tcacgcgatt
    53101 atccgcaggc ttcgcgcctg ttctgtatgg agatgctggc aggcgcgccg ttgttaatgg
    53161 atgaactgac gggcgatttg aaggcattaa ttgatgagaa atcggcgctg attgccggtt
    53221 gggtcaaaag cggcaaactc gcgccgattg atccgcagca tttgattttt atgatttggg
    53281 cttccactca acattacgcc gatttcgccc ctcaggtgga ggcggtgaca ggcgcgacgt
    53341 tgcgcgatga ggtatttttc aatcaaacgg ttgaaaacgt gcagcggatt attattgagg
    53401 ggattcgacc acgttaaaga tgccggagga ggttgtaaca tcctccggct acctgtttaa
    53461 cctatagtca ttaagctggc gttaccgcca gcggcagcgg tattcacact cagcgaacgc
    53521 tcgatataca gccgttccag aaggatattg ctttcgccac gggcaaaacc ctgcaccgaa
    53581 acaattgcgc catcccgcgc ggcaacggct tcacacaatg cgcgaagctg atccgaatca
    53641 ccgtggaaga tcaccgcatc aaacggttga gcggttatat tttccgcttt cgccagttga
    53701 atacgttcgc tgactgccga tggcaatgcc ttcactagct gacgatgcag cgcgtcatcc
    53761 ggccacagta cctggctgcc caccgccagc acggcggcga gctgagtcag cgcatcctgc
    53821 tcatcatcgg caatacacaa cacgcgctca cgcggcagca gcgtccaggt gttgcgttca
    53881 cccgtcggcc ccggcagcaa tcgttgtgtt cctgcctgcg ccagctcgcc atattgcgta
    53941 cataacgcct gcaattctgg acgatttgct gcccattccc gcagtgcatt tagcggctga
    54001 gtcaatgcgg ctttcaactg tgcatcgacc ggatactctg catcctgacg cgcgagcgtc
    54061 actgccagcg cactttccgg gcgattcgcc agcagacggt agagatagag cggaccgcct
    54121 gctttcggcc cggtaccgga caacccttcg ccgccgaatg gctgcacacc caccactgcg
    54181 cccaccatat tatggttaac atacaggtta ccaacatggg ccgagccagt gacctgggcg
    54241 atggtttcat caatacgcgt atggacacca agcgtcagac catagccgga agcgttaatc
    54301 tgctcgatca gctctggtag ctggttacgg ttgtaacgca ccacatgcag caccggacca
    54361 aagacctctt tttgcaattc ggcaaagtca tccagttcga tcagcgtcgg ggcgacaaag
    54421 gtgccgcttt gccattcacg ggcatcttcg ctgttttccc gcaccgcctg gaacaccgga
    54481 cggcctttgc tacgcatggt ctgaatatgg cgctcaatat tggctttcgc ttcgctatca
    54541 atcaccggac cgatatcggt ggtcaggcga cccggattac ccatccggca ttcggccatt
    54601 gcaccgcgca gcattttcaa cgtgtggtcg gcaatctcat cttgcaggca cagcacgcgc
    54661 agtgccgaac aacgctgacc cgcactgtcg aacgccgagg ccagcacatc cacgacgact
    54721 tgttcggtca gtgctgaaga atcgacaatc atcgcgttca tgccgccggt ttcagcgatg
    54781 agcggaatag ggcgaccctg agcgtccagg cggctggcga tattacgttg cagtaacgta
    54841 gcgacttcgg ttgaaccggt aaacatcacc ccgcgcacgc gatcatcacc cgtcagttgc
    54901 gcgcccacgg tttccccctg acctggcagc aattgcacca cgccaggggg tacacccgct
    54961 tccagcaaaa tggcgatccc ttgcgcggca atcagcggcg tttgttctgc cggttttgcc
    55021 agcacgctgt tacctgccgc cagtgcggca gcgatctgcc cggtgaaaat agccagcggg
    55081 aagttccacg gactgataca caccacaggc cctaatggac ggtgggtttc gttagcgaaa
    55141 tcatcccgca cctgtccggc gtagtagtgg agaaaatcga ccgcttcgcg cacttcggca
    55201 atggcgttac tgaaggtttt tccggcttca cgcaccagaa taccaatcag ttgctgcatc
    55261 tggctttcca ttagcacggc agcgcgatgc aaaatcgccg cgcgttcagc cggaggcgtg
    55321 gcaaaccaga ttggcgcgtt attaaccgca ctttccagcg cctgttctac ttcacgcggc
    55381 gtggcttcac gcacaaagcc cacaatatct ttcggttccg cagggttaat aacgggcgac
    55441 atctcacctg ccgctaccgg ttgttccagc attggcaagg cctgccattt ttgcagtgca
    55501 ctattgagca gggcagagga gagcgaggcc agacggtgtt cgttagcgag atccagccct
    55561 gccgagttgt cgcgcccgtg accgtaaaga tcgcgcggca ggggaatttt cggatgcggt
    55621 aatccagttt gcccttcctg ttgcgccagt ttttctacag cagtgaccgg atcggcgacc
    55681 agttcatcca gtggcaaaga ggtgtcggca atacggttaa caaacgaggt gttagcaccg
    55741 ttttccagca ggcgacgcac cagatacgcc agcagcgttt catgtgtgcc aaccggggca
    55801 taaatacgac acggtcggtt aagtttaccg tcggcaactt tcccggtgac ctgctcgtac
    55861 agtggctcgc ccataccatg caggcactgg aactcgtact gacccggata gtagttctga
    55921 cctgccagtt gataaatcgc tgccagcgta tgggcgttgt gcgtcgcgaa ctgcgggtag
    55981 attagattcg gcaccgccag cagttttttc gcacaggcga gataagaaac gtcggtatac
    56041 accttgcggg tataaaccgg ataaccttca aggccgtcca tctgcgcacg cttaatttca
    56101 ctatcccagt acgcgccttt caccagacga atcatcagac ggcgacggct acgggtggcg
    56161 agatcaatca ggtaatcaat caccaacggg cagcgttttt gataagcctg aataacaaaa
    56221 ccgatgccgt tccagcctgc cagttccggc tcgaaacaga gtttttccag cagatcgagg
    56281 gagatctcca gacgatcggc ctcttcggca tcgatgttaa taccaatatc atactgacgc
    56341 gccagcaggg tgagtgattt cagacgcggg taaagttctt ccattacccg gtcatactgg
    56401 gcgcggctat aacgcggatg cagcgccgac agtttgattg aaatgcccgg cccttcatag
    56461 atgccacgac cgttagacgc tttaccgatg gcgtgaatcg cctgctgata ggaaaccata
    56521 tacgcctgtg cgtctgcggc agtcagtgca gcttcgccca gcatatcgta agagtaacgg
    56581 aaacctttct cttccagctt gcgggcattg gctaacgctt ccgcgatggt ttcacccgtg
    56641 acgaactgct cgcccatcag gcgcatcgcc atatccacac ctttgcggat cagcggttca
    56701 ccgcttttac cgataatgcg gttcagcgag cgggagagga tggcttcgtt atgggtggaa
    56761 accagtttgc cagtaaacag cagcccccag gtggcggcat taacaaacag tgacgggcta
    56821 cgaccaatgt gtgactgcca gttaccgttg ctgattttgt cgcgaattaa cgcgtcgcgg
    56881 gtggctttgt cgggaatacg caacaacgct tctgccagac acatcagcgc cacgccttcc
    56941 tgcgatgaca gcgaaaactc ctgcaataac ccctggacca tacctgcgcg accactggca
    57001 tttttttgat tacgcagttt atcggccagt tgatacgcca gtttgtgcgc ctgtgcagca
    57061 actggctgcg gcaggcgggc ttgttccagc agcatagaaa ccgcttcggt ttccgggcga
    57121 cgataggccg cggtgatcgc ggcgcgggaa accgactggg gcaaaatttg ctcggcaaag
    57181 tcgaggaatg gctggtgtgg ttcctctgcc ggagtcggtg cttcatcgct ctcattggca
    57241 gcgccagaaa gcagcgcagg tagctctggc agagtatcgc tgttttccag ttgttcgaga
    57301 taagaaaaaa tcgcctgctt aattaaccag tgtggtgtgc gatcgatacg tgtcgcggca
    57361 gacttaatac gctcacgcgt cgcgtcgtcc agcttaaccc ccatggtggt ggttcccatg
    57421 ccattactcc tgttgttctg aaaggtgcaa cttaacgtta tcgtgaaata tccatgatgt
    57481 tgcaactttg tgcaaccatg ttaaatgtga cctgcgtagc aagcttaaaa atgaatgaaa
    57541 tgttaataaa agaaatcgat atgacaggga ttaaaaaaat aaatcagact ttttctctgc
    57601 ggcagttaac atttttgaaa ggtgcaaccg caaaaaatgt gagagagtgc aacctgatga
    57661 aaaatagtgt cgctgagcac taaaatttaa tgtaaatggt gtgttaaatc gattgtgaat
    57721 aaccagcgct tccggcagga tacggtcgcc ctggtaaaac ataaactctg ttaccccgtt
    57781 ccggtggcag atataacggc aagtttcgac attgccgata ataatttttt ggagacttta
    57841 gatggctatt agcacaccga tgttggtgac attttgtgtc tatatctttg gcatgatatt
    57901 gattgggttt atcgcctggc gatcaacgaa aaactttgac gactatattc tgggcggtcg
    57961 tagtcttggg ccattcgtga cggcattatc ggcgggtgcg tcggatatga gcggctggct
    58021 gttaatgggg ttgccgggcg cggtttttct ttccgggatt tccgaaagct ggatcgccat
    58081 tggcctgaca ttaggcgcgt ggatcaactg gaagctggtg gccgggcggt tgcgtgtgca
    58141 taccgaatac aacaataacg ccttaaccct gccggattat ttcaccgggc gctttgaaga
    58201 taaaagccgc attttgcgca ttatctccgc gctggttatt ttgctgttct tcaccattta
    58261 ttgcgcttcg ggcattgtgg caggcgcgcg tctgtttgaa agtacctttg gcatgagcta
    58321 cgaaacggct ctgtgggcgg gagctgcggc gacgatcctt tacaccttta ttggcggttt
    58381 cctcgcggtg agctggactg atactgtaca ggccagcctg atgatttttg ccctgatcct
    58441 gacaccggtt atcgtcatta tcagtgtcgg tggctttggt gactcgctgg aagtgatcaa
    58501 acaaaagagc atcgaaaacg tggatatgct caaagggctg aactttgttg ccattatctc
    58561 attgatgggt tgggggctgg gttacttcgg tcagcctcat attctggcgc gttttatggc
    58621 ggcggattct caccacagca ttgtccatgc gcgtcgtatc agtatgacct ggatgatcct
    58681 ctgcctggca ggggcggtgg ctgtcggctt ctttgggatt gcttacttta atgagcaccc
    58741 ggcggtagca ggtgcggtaa accagaacgc tgaacgtgtg tttatcgaac tggcgcaaat
    58801 tctgtttaac ccgtggattg ccgggattct gctgtcggca attctggcgg cggtaatgtc
    58861 aacgttaagc tgccagctgc tggtgtgctc cagtgcgatt accgaagatt tatacaaagc
    58921 gtttctgcgt aaacatgcca gccagaaaga gctggtgtgg gtagggcgtg tgatggtgct
    58981 ggtggtggcg ctggtggcga ttgcgctggc ggcaaacccg gaaaaccgcg tgctgggctt
    59041 agtgagctac gcgtgggcag gctttggcgc ggcgtttggt ccggtggtgc tgttctcggt
    59101 gatgtggtca cgcatgacgc gcaacggtgc gctggcgggg atgatcatcg gtgcgctgac
    59161 ggttatcgtc tggaagcagt tcggctggct gggactctac gaaattattc cgggctttat
    59221 cttcggcagt attgggattg tagtgtttag tttgctgggt aaagcgccgt cagcggcgat
    59281 gcaaaaacgc tttgccgagg ccgatgcgca ctatcattcg gctccgccgt cacggttgca
    59341 ggaaagctaa gggacttagc ctgcggcggt tttgtttggc ttcagcagcg ggttgcgctc
    59401 ccttaatgtg cctcgccata taaattgaat ggtgcaggga gcgcgcaggg ggcggccaat
    59461 cgccgccgcc ccctgctgtc ccggccttcg gggaacgctt cagcgatttt gacgccacca
    59521 acacccgagc tgttattatg ttccgggcaa aaagttagat ttgataatcg cggatggacg
    59581 aaattgcttg atacacccgc ttatcagttt tacatggaag ctctgatgca ttgagtctgg
    59641 acagttttgt cggctggata cggcgtttac gcggcatccg gcaagaacac atggttcttt
    59701 gcaaacaatc ccatctttct accctggaat aatcgtttat atcccttggc attacctctc
    59761 tttgtttaca ttccaacatc attttataaa cattccgctt gtatttttct ttaccgtaat
    59821 gataatcgct atcactgcga tttacttttc tttgcataga ttgactcaga aaaacgttta
    59881 agggtgggtg gcatgtttgt tccgtttctc attatgttgc gcgaaggact tgaagccgcg
    59941 ctgattgtca gtttgattgc cagctatctt aagcgtaccc agcgaggccg atggattggt
    60001 gtgatgtgga ttggcgtgtt gcttgccgct gcgttgtgcc tgggcctggg tatcttcatt
    60061 aacgaaacca ccggcgaatt tccgcaaaaa gaacaggaac tgtttgaagg tatcgtggcg
    60121 gtgatcgccg tggtgatcct tacctggatg gttttctgga tgcgcaaagt gtcgcgcaac
    60181 gtcaaagtgc aactggaaca ggcagtcgat agcgcattgc agcgtggaaa tcatcatggc
    60241 tgggcgctgg tgatgatggt cttttttgcc gttgcaaggg aagggctgga gtcggtcttt
    60301 ttcctgctgg cggcatttca acaagatgtc gggatctggc cgccgctggg tgcaatgctc
    60361 ggtcttgcta ctgccgtggt gctcggcttc ctgctctact ggggcggtat tcgcctcaat
    60421 cttggtgcat tttttaaatg gaccagcctg tttattctct tcgtcgccgc agggctggcg
    60481 gctggtgcca ttcgcgcatt tcatgaagcc ggattgtgga accactttca ggaaatcgcc
    60541 ttcgatatga gcgcggtcct ctcaactcac tcgctgtttg gcacgctgat ggaagggatt
    60601 tttggctatc aggaagcacc gagcgtcagc gaagtcgccg tctggtttat ttatctcatc
    60661 ccggcgctgg tggcatttgc cctgccacca cgcgcagggg cgacagcgtc tcgctccgcg
    60721 tagcaaatac gacgcaaact cttgcttagt tacaacatac tttaaaggga tagtctcgtc
    60781 atgaccatta acttccgccg taacgcattg cagttgagcg tggctgcgct gttctcttct
    60841 gcttttatgg ctaacgccgc taatgtgccg caggtcaagg tgaccgtgac cgataagcag
    60901 tgcgaaccga tgaccattac ggttaacgcc gggaaaacac agttcattat tcagaaccac
    60961 agccagaagg cgctggagtg ggagatcctc aaaggcgtga tggtggtgga agagcgggaa
    61021 aatatcgccc ctggctttag ccagaaaatg acggcgaatt tacagcctgg cgaatacgat
    61081 atgacctgcg gtctgctgac taacccgaaa gggaagttga tcgtcaaagg tgaggcaacg
    61141 gcggatgcgg cgcaaagtga tgcgctgtta agtcttggtg gtgcaattac tgcatataaa
    61201 gcgtatgtca tggcggaaac cacgcagctg gtgaccgaca cgaaagcctt tactgacgct
    61261 attaaagcag gcgatatcga aaaagcgaaa gcactgtatg cgccgacgcg ccagcactat
    61321 gagcgcatcg aaccgattgc tgaactgttc tccgatctgg atggcagcat tgacgcccgt
    61381 gaagatgatt acgagcaaaa agccgccgat ccgaaattca ccggtttcca ccgtctggaa
    61441 aaagcattgt ttggcgacaa caccaccaaa ggcatggatc agtacgctga ccagctttat
    61501 accgatgtgg tcgatttgca aaaacgcatc agtgaactgg ctttcccacc ttcaaaagtg
    61561 gtcggcggtg cagccggact gattgaggaa gtggcagcca gcaaaatcag cggtgaagaa
    61621 gatcgctaca gccacaccga tctgtgggat ttccaggcta acgttgaagg ctcgcagaaa
    61681 attgtcgatc tgctgcgtcc acaactgcaa aaagccaacc cggaactgtt ggcaaaagtc
    61741 gatgccaact ttaaaaaggt cgataccatt ctggcgaaat accgtactaa agacggtttt
    61801 gaaacctacg acaaattgac cgatgccgac cggaatgcac tgaaaggacc gattactgcg
    61861 ctggcggaag atctggcgca acttcgcggt gtgctgggat tggattaagc attatgcagt
    61921 atgaagataa aaacggcgtg aatgaaccgt cacgccgacg tttactgaaa ggcattggtg
    61981 cgctggcgct ggcgggaagt tgtccggtcg ctcatgcaca aaaaacgcaa agtgcgccgg
    62041 gtacgctttc accggatgca cgcaatgaga aacagccgtt ttatggtgag catcaggcag
    62101 ggatcctgac gccacaacag gccgcaatga tgctggtggc gtttgatgtg cttgccagcg
    62161 ataaacccga tcttgaacgg ttgtttcgct tgttgactca gcgttttgct tttctgactc
    62221 agggcggagc ggcaccagaa acgccaaatc cgcgcctgcc accactcgat tccggcattc
    62281 ttggcggcta cattgcgccc gataatctca ccatcacgtt atcggtgggt cactcattgt
    62341 ttgatgagcg ctttggcctt gcgccacaga tgccgaaaaa gttgcagaag atgacgcgtt
    62401 ttcccaacga ctcgctggat gcggcgttat gtcatggtga tgtgttgcta caaatttgcg
    62461 ccaacaccca ggacacggtg atccatgcgc tgcgcgatat catcaaacat acgccagatt
    62521 tgctcagcgt gcgctggaag cgggaagggt ttatttccga tcacgcggcg cgtagtaaag
    62581 gcaaagagac gccgattaat ttgctgggtt tcaaagacgg cactgccaat cccgatagcc
    62641 agaatgataa gttgatgcaa aaagtggtgt gggtaacggc agatcagcag gagccagcgt
    62701 ggacaatcgg tggcagctat caggcagtac gcttgattca gtttcgagtg gaattttggg
    62761 acagaactcc gctgaaagaa cagcagacga tttttggtcg tgataagcaa accggtgcgc
    62821 cgctgggaat gcagcatgag catgatgttc ccgattacgc cagcgatccg gaagggaagg
    62881 tgattgcgct ggacagccat atccggctgg cgaatccccg cacggcggag agtgagtcca
    62941 gcctgatgct gcgtcgtggc tacagttatt cactgggcgt caccaactcc gggcaactgg
    63001 atatggggct actgtttgtc tgttaccaac acgatctgga aaaaggcttc ctgactgtac
    63061 aaaaaaggct caatggcgaa gcgctggagg aatacgttaa acctatcggc ggcggttatt
    63121 tttttgcgct gccgggagtg aaggacgcga atgattatct cggaagcgcg ttattgcggg
    63181 tttaatgttt atagacggat aaggcgttct tgcgcagatg cctgatgcga tgcttgcgcg
    63241 tcttcttatg cctacaatcg gtgcgggttc ggtaggcgga taaggcgttc acgccgcatc
    63301 cggcgttcgt gcgcagatgc ctgatgcgaa tcctgctgaa agcacacagc ttttttcata
    63361 tctgtcatca ctctgtcatc tttccagtag aaactaatgt cactgaaatg gtgttttatg
    63421 gttaaatata agtgaatata ttgttgcaat gaatgcgaga tctgttgtaa ttattaagta
    63481 gcagcggaag ttcccggcag tgatagtcag tcactatgga gatcgcggat ggtaacgtcc
    63541 tgttctggac aatgtcttgt taagctacgc tccaccactt gcggagattt ctcctccggt
    63601 agcaacttcg tcactctgta tttttctcac ttcctctctt tactcgtgtt accgacgccg
    63661 caaacggtgt gcgtagccgt ttttcggcat tttatccaaa agcaaactat ggcttacaag
    63721 gaagccaacc ctctgatgtt cgtgcgcata atcgcgctgc caacggcgcg tgtgatgaat
    63781 acaaacaact caaggtgctc tccatgggaa gacaaaaagc agtgatcaaa gctcgtcgtg
    63841 aggcaaaacg tgtgctgaga cgggattcgc gcagccataa gcagcgtgaa gaagaatcgg
    63901 tcacctcgct tgtgcagatg ggcggcgtag aagccattgg tatggcccgc gacagtcgcg
    63961 atacttcgcc catcctcgcg cgaaatgaag cgcaattgca ctatctgaag gctattgaga
    64021 gtaagcagct gatattcgcc acgggcgaag ccgggtgcgg aaaaacctgg atcagcgcag
    64081 caaaagcggc agaggccctg atacataagg atgtcgacag gattatcgtc acccgtccag
    64141 ttctgcaagc cgatgaagat cttggcttct tacctggaga tatcgcagaa aagtttgctc
    64201 cctattttcg cccggtctac gacgtgctgg tccggcgctt aggggcttcc tttatgcagt
    64261 actgcctgcg accggaaata gggaaggtgg aaattgcgcc gttcgcctat atgcgtggac
    64321 gtacctttga aaatgcagtc gtcattcttg acgaggcgca gaatgtgact gccgcgcaaa
    64381 tgaaaatgtt tttaacccgc ctcggggaga acgtgacggt tatcgtcaac ggtgatatca
    64441 cgcaatgcga tttgcctcgc ggcgtgtgct ccggattaag tgacgcgctg gaacgtttcg
    64501 aagaagatga aatggttggg atcgtccggt tcggtaaaga ggactgcgta cgttcggcac
    64561 tttgccaacg tacgctgcat gcatacagtt aagtgtatta tcggtgcaga gcccgggcga
    64621 accgggcttt gttttgggtg tttatgcccg gactagcgct ttttctgaaa caaccatttt
    64681 tatttgcccc tggctggtga agtgtacgct catcctgtgg cttttttgta gttgctgata
    64741 gagctcatca ggtattgcta agctctctgc atattcttgc ggcgtatatt ggtaggctgc
    64801 atgatgctgc tgtttttgaa aacgcagctt attgtacagc gcccagacaa ttaacacgac
    64861 ggcattcgcc actgccagca aaaaatagaa ctgaagtcgg cttctggcct cgctttgcca
    64921 gtaataaccc gtcagcagat ccatggcgaa taagaatatg aacaacgcaa ataatgtcca
    64981 caagatggtt gtggcaacat aatcaaccag taaacgtact ggtgattgtc gggtcgtaat
    65041 aattaaattg ttcatattaa cctctcagaa tcccgcgatc gggacttacc caacgggcgc
    65101 gttgcttttt aggcatcaac atgactcgtg taaatgatac caatgtcgtt gccaggctca
    65161 gcatccagaa aataaccggg aaccaaataa tccagaaaag cgatgaagtc agattatgct
    65221 catagcgatt ctcgatcatc aggctgacaa taaattgcag taaacataac gtacacaata
    65281 atattccggc agtatgtgtc gcagcgatat gtgtcaattc aatatttaac ggtacaccgg
    65341 caagttggac tgcgtaaata atgaaaccga ccaggcaggt gaaggcccat attgtcgtca
    65401 ggcagtattc aaaaaacagc ggccacattc gaaagttttc tttgcgccac aaccttgtca
    65461 tatttttgag gaatacttct gcaccgccct gagcccagcg caggcgctgt ttccacagcc
    65521 cttttaacgt ttcaggcatt aatatccagc acagcgcccg tggctcgtaa aaaatcgtcc
    65581 actgattcaa ctgcagcttc cagctaatat caatatcttc ggtgatcata tcgtcactcc
    65641 agtaacccac ttctgccagg gcgctgcgac gaaatgcggc aataacaccg gaaacggtaa
    65701 atacgtttcc atagatacgc tgggttcgct tgatcaaacc aataattgag gaatactcgc
    65761 caacctgaat tttacccacc agggtagaac gtgttcgaat acgaggatta ccggttacgg
    65821 cacccacacg cgggttgtac aacatcggtt ccacaatata tgccgccgca tcgcggtcta
    65881 ataacgcatc gccatcaatg cacaccagat attcactttt cgccgcggca gctccggttt
    65941 taagcgcaat ggctttcccc tggttttgcg ccagatgaat gacccgcaaa tggggaattt
    66001 gtgcagccat gcgatccagg atggcacggg ttttatctgt tgaaccgtca tttacggcaa
    66061 taacttcaat gttctcataa cgctgtgcta aagcggcgtg tatggtttcc tcaacgtttt
    66121 tctcctcatt aaaacaggga atgataatgg agatagacgg attatctttc aactggggag
    66181 ctggtgcgtt ttctccccac ggccagtggc gttcacgata gacccagaaa tagacgccgc
    66241 caacaatcca cataatggac ataaaaaacg gccagaagaa aacgaacctc atcatcagtt
    66301 caccagagtg aaagtacgct acgcataggg gtatgcataa caccagacat aatataaaaa
    66361 acgatacgat gcgattaatc atttttcgga taccaggctg ttgaaaactc aggacgaata
    66421 aggtctattt caggttggtt atgcagaaaa ttgtcgggat aataaccata gtttttcact
    66481 ccattcagtt gtaataggct catccagtga gcgagttgtt gcgaagaaat agcctgatgc
    66541 tgaccatttt tctgccagtt ttgtgcctgt aattctaaaa tagatttgtc tttagcctga
    66601 gggatgtttt taatttgatt ggtcaattgt attaaccatt ggtcagccga tttttctgcg
    66661 acaccttcca gataaggcat agccataata gcggtccagt catagctttt taggaaatca
    66721 gcataattct gtgcaaacca ggcttcactt tcaggttgta ttaccggaag tgcaaaaata
    66781 tttcgtgcag ttttaatatg tggaccgcga atggctttta cgcgcgcact aagttctaaa
    66841 gtgaagtcag ttaacgcacg acttttaaag cgggcccact gtttaaattg ctccgggttt
    66901 tgtcgaattt cgctcagact cccgctaaag cctgcttgct gataagccgt gatagccggt
    66961 gcactggcat cttcataatc tgaaagcaaa gcatcatcgt ggaacaatat gccatcaaaa
    67021 gcagcatgtc cggcaagatc ttcatataac atgccaactt gtgctctgac tctgtcatcg
    67081 aaaggagaga gacggtgata ttgttcagga tgaatttgtg cttttttctc ccctgttggt
    67141 aagtatttta ctcgcgttaa tgtgggatct aaatcccagc ttaataccgg catccacgca
    67201 tagatgttta cacctgagcg ggtacgtaat tgccaggcaa cccgactaaa aatatctgct
    67261 ttcattggta gcaaacgatt tggaaaccag acctctttga ccagcccatc accatcggga
    67321 tcagcaaatg cctgcaaata cacggttgat atttgcatat ctttcacccg ctgaattagc
    67381 acatcaatat tgcgatccat ttgctggagg ttttcgtcat aaacgtaatc aagatcgata
    67441 tgcattatcc gttgtggtga tttttcctgt acggtaataa tttgctgggc aaactctttt
    67501 aatgagggat tattggcgat taatacccgc ggaatggaat ccaattgcga cgcatttgcc
    67561 aaacctgatt caagggtgaa gaacatgtca taaccgagtt tttttaattc ctctatcgct
    67621 atgccattcg cttcgccata aggccaaaca aaaacgtgtg gatttacctc aacctttgta
    67681 cgcaggtatt ccgtcatttt tacagcatcc agacgaattc tttcccggta ttctgctgcg
    67741 gtttcatacc gtgcgtggtc agtaaaatat gcacgattta catatacagg caataagctg
    67801 ccggtggcat tagcctgaat accgtagtga gaattccatg tatgagaagc gagctcaacg
    67861 agccgggaac gcgcaacttc tcgcacttgt tgccacgtgg caaaatattc tcgatcgacc
    67921 aactcatcgc caaattttac ttgtttatcc gctggcgtat cgacccaact gccgacgggg
    67981 gcccatacag caggccactg gaaggcctga agaattggga agacgcgggt ataaaaactc
    68041 tggtagccgt catcaaaagt cagcactaca gctttttccg gtagcggttt tcctcctcga
    68101 tgtgcttcac gaatttgagc aatactgacc ggttgataac cgttctcgcg cagccaggca
    68161 aattgttcac gcagtgctga tgtccgcact gacataaaac gctggtcggc agcttcgtct
    68221 tcaacgttat gccatgaaat cgctacaaaa ccattatgcg gccacggttg ctcggcgagt
    68281 aaagattcgc gatcctgtgg cggtataaat gatgttcttg actggctaat gcacgcggtg
    68341 agcataatta tactcaccag catcaggaga tatttatttc cattacgtaa catatttatc
    68401 cttaaaatct gaatgtcata tcgaattcaa cgtataagtt gtgttctctg tcaccgtcat
    68461 aaggtcgttt ttcccagcgt agcgttgcgc cagcatcaat cacgtcattc cagctaatgc
    68521 gttgcccgta gccgagttgg gtgacgacat ccgtgccata atgtttttgc caggaggcac
    68581 caacacctgc gctgaatatt tgctcccagc tattttcata gcttcgccat aacaaatggc
    68641 ttgcctcaaa tgccggaaca atatcgaacg tttttatagg gttgtagtat ggggtatcgt
    68701 gttctgtatt ttgttcgtaa tacagactgg gtaggaaatc gacaatcaaa tatggtgaag
    68761 accagatgcg ttcctgaccc tcaagtgaga cttcatgacg ctggttactg tcggaaaaat
    68821 cagtgaaagc ccaggagaca ccgtacttac gccgctcatt ttgataccag cgaacataag
    68881 cctgagcact gttgcctgta acaccatttt tcattgcccg taatggaacg cggtgagaga
    68941 ggcgttccag ttgcgaacca atacgccagt tatcattaaa atcataccag ccagacaggc
    69001 gcgcgccggg tttatgctca tgattgaaaa cgcgttcagc gtactctgcc tcgagccaga
    69061 tattacgtga ccgccactca acacccgcaa gccagtcgcg aacaatccct tttccttcgc
    69121 taaattgtcc atcggcataa ccgaatccag caaaaccgcg ccagttatcc ttcagcggtg
    69181 gtgaataaac gatggtggtt aagtcgacat catgtttacc actatccggg ccttcggcat
    69241 caattcctgt tgagccagcg atacgaagct ctgcaagatt atgtacatca accgcacgtt
    69301 ttaatcgtac aacgccggga tcttgcggtt cacgttcgac aacatcgtgc gttaagacag
    69361 ctgcctgctg ccattcttgt aacgttaatg ctgtccaggc ttgttcaacc tccagattaa
    69421 tattacgtgg ctcgatcact tctgcttttt ttaattcatt ttctgctgca cgaggccaac
    69481 cgcgggcttg taacacactc gcgtaatcaa tgcgcagtcc ctgatttcct ggtgcgttat
    69541 aagcaagctc tctggctgtc atttcagcct gaggaagatc attactatat tttgctacgg
    69601 ttgagagaaa cgaatgcccc tgtaaccagg tatcattcgg gatgctcgta ggcgtgccca
    69661 ttaaccgaag gaaaggcggc gaagtattaa tggtatgttg ggtgacagtt agcgcgcccg
    69721 gataattttc actctccagg tggctgtaaa agagatccgc aagttcttca tcggataaat
    69781 ccggggcaat ggtctccttg tgataaaaga gctcggtcat tattgactgt gcttttttcg
    69841 gctgatgatc tttgagataa gccgatgcaa cccaatattg cccccagggc ggaataattt
    69901 gccccgtctt ttttaatcgc tgatagtgag aaataacgtc tttataacga tcgcgagtta
    69961 ataacgcgcc aagatgatca acctgaatac gctggtactg ggcagtacgg tctgggttat
    70021 cgtgccacag aatttctaat gcagcgtatt gggcgagggc gcgatcggca atggcataac
    70081 gttcactttc actgcgcgta ggcataaacg acagtctgac cagttcggca tgaatatcag
    70141 cgcgaatatc tggcgttaaa ttggcatcgt caatcgcggc agcaagttga ttattacgta
    70201 atgcctgcac gtattctgtg ggatattgtt gcgtagatgc attttcaggt aatgactctg
    70261 tcatcgcccg taattcatcc tgatgacgcc ccgccagttt atagatatag gcttctgcga
    70321 gtaaattggc tttgtccggt gctccagagt taagctgctt aagtttaacc agcgcagtat
    70381 catagtgacc agcatctgcc agggttaaaa tttgtccccg ttgataatcc ttattttgcg
    70441 gctccagaga gagcgccttt tgccacagtg taagcgagtt ttgccattgt tgcaggttac
    70501 gataagcgac ggcgacagct gcataaccac gcgctggtaa ttgctgatga cggtagcggt
    70561 tgtaaacggt aataacctgt ttatcttgcc cggcccataa ggcaatctgt aaccagtcag
    70621 caatttggtt attgctgagt gctgatttta gtgcaaacca tgacaaagct ggctgagtat
    70681 tacccttgcg agcttcaata atcaatgcat cataggcgtt attaacagca ctcttcgccg
    70741 cgggactcgc tgctaaaaat gcggcagtaa gaagtttcaa agcccatttg gttttcgggc
    70801 acctttttct gctacttgaa tacatcctgt attactccat gtattgccaa aatctctctc
    70861 tgtatctaat tacaggtaac tgaaaagaaa gatatttttg cacctcataa tccgttatta
    70921 aacgcggaag agagacgtga attgttgatg atgagaagaa gaaatgatga gcagagtgtc
    70981 catataaaat ccttttctcg cccgaaaatc cattccaatg atgaggatct tcaggaatac
    71041 ggcataaatc ccaatgcctt tttcaaaata aattaggatt aaaataatta aatcagtaaa
    71101 ttccgatgca tgatttcgga tttttcgaaa gtcctgatga aaggctgcgc tacggcactc
    71161 acggatttaa ttgttattcc taatgtatct atgaattaat gttttataag gattttctat
    71221 aaccatttat aagtattttc aagcctggct tgttgcaaac aatgtataaa gcacttaggc
    71281 aataataatt acattcagca actatcatca tcggtattgt ttgtgggcgg aatttcatat
    71341 tgacaacagt acagttctta tatctattaa taatagaaag ggatctaaaa cctacagatt
    71401 ggtgtagctt tatggaaaaa gactatttga gaattagtag tactgtatta gtgagcttat
    71461 tgtttgggct tgctttggtt ttggtgaata gttggtttaa tcagccaggc gttgaggaag
    71521 ttgttccacg atcaacctat ctgatggtaa tgattgcttt attttttatc gatactgttg
    71581 catttatttt tatgcagttg tatttcattt atgaccgtag gcaattttca aactgtatac
    71641 ttagtttggc ttttctgagt tgtttgattt actttgttaa aaccgtcatt atcattcagc
    71701 aaattattga ggggcgtttg acaagcagtg ttgtccaaaa tgacattgca atctattatt
    71761 tgtttcgtca gatgagtttg tgcatattaa tatttctggc attggtgaat aaagttagtg
    71821 aaaacacaaa acagcgcaat ttattttcca aaaaaatgac tttgtgcata agtttgtttt
    71881 ttgtttttgg ggggccgata gttgctcaca tactttctag tcattatgag agctacaatt
    71941 tacacattgc cgagttaacc aatgaaaatg gtaaggtggt ttggaaagcc tcatatgtta
    72001 ccataatgat tttcatgtgg ttaaccttgc tttcggttaa tctatacttt aatggtttac
    72061 gctatgatat ctggaatgga gtgacggtga ttgctttttg tgccgtactg tacaatatct
    72121 ctctgttatt tatgagtagg tatagcgtgt ccacttggta tattagtcgc actattgaag
    72181 tagtcagtaa gttaaccgtt atggtaatat ttatgtgcca tatattcagt gcgctacgag
    72241 taacaaagaa catt
//
LOCUS       DAZGSP010000009        69778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZGSP010000009 DAZGSP010000000
VERSION     DAZGSP010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.009"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..904)
                     /locus_tag="ACHK74_000693"
     CDS             complement(<1..904)
                     /locus_tag="ACHK74_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFP0219566.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLS"
     gene            1240..2442
                     /gene="nupC"
                     /locus_tag="ACHK74_000694"
     CDS             1240..2442
                     /gene="nupC"
                     /locus_tag="ACHK74_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFP0219567.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(2492..4681)
                     /gene="pdeA"
                     /locus_tag="ACHK74_000695"
     CDS             complement(2492..4681)
                     /gene="pdeA"
                     /locus_tag="ACHK74_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFP0219568.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLSLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQSVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(4889..4964)
                     /locus_tag="ACHK74_000696"
     tRNA            complement(4889..4964)
                     /locus_tag="ACHK74_000696"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4929..4931),aa:Ala,seq:ggc)
     gene            complement(5004..5079)
                     /locus_tag="ACHK74_000697"
     tRNA            complement(5004..5079)
                     /locus_tag="ACHK74_000697"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5044..5046),aa:Ala,seq:ggc)
     gene            5300..5659
                     /gene="yfeC"
                     /locus_tag="ACHK74_000698"
     CDS             5300..5659
                     /gene="yfeC"
                     /locus_tag="ACHK74_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP0219569.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            5661..6053
                     /gene="yfeD"
                     /locus_tag="ACHK74_000699"
     CDS             5661..6053
                     /gene="yfeD"
                     /locus_tag="ACHK74_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP0219570.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(6105..7520)
                     /gene="gltX"
                     /locus_tag="ACHK74_000700"
     CDS             complement(6105..7520)
                     /gene="gltX"
                     /locus_tag="ACHK74_000700"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFP0219571.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            7779..7854
                     /locus_tag="ACHK74_000701"
     tRNA            7779..7854
                     /locus_tag="ACHK74_000701"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7812..7814,aa:Val,seq:tac)
     gene            7899..7974
                     /locus_tag="ACHK74_000702"
     tRNA            7899..7974
                     /locus_tag="ACHK74_000702"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7932..7934,aa:Val,seq:tac)
     gene            8021..8096
                     /locus_tag="ACHK74_000703"
     tRNA            8021..8096
                     /locus_tag="ACHK74_000703"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8054..8056,aa:Val,seq:tac)
     gene            8101..8176
                     /locus_tag="ACHK74_000704"
     tRNA            8101..8176
                     /locus_tag="ACHK74_000704"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8134..8136,aa:Lys,seq:ttt)
     gene            8207..8332
                     /locus_tag="ACHK74_000705"
     ncRNA           8207..8332
                     /ncRNA_class="other"
                     /locus_tag="ACHK74_000705"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(8441..9325)
                     /gene="xapR"
                     /locus_tag="ACHK74_000706"
     CDS             complement(8441..9325)
                     /gene="xapR"
                     /locus_tag="ACHK74_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFP0219572.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(9366..9524)
                     /locus_tag="ACHK74_000707"
     CDS             complement(9366..9524)
                     /locus_tag="ACHK74_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFP0219573.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(9577..10833)
                     /gene="xapB"
                     /locus_tag="ACHK74_000708"
     CDS             complement(9577..10833)
                     /gene="xapB"
                     /locus_tag="ACHK74_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFP0219574.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKAVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            complement(10893..11726)
                     /gene="xapA"
                     /locus_tag="ACHK74_000709"
     CDS             complement(10893..11726)
                     /gene="xapA"
                     /locus_tag="ACHK74_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFP0219575.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            11975..12739
                     /gene="yfeN"
                     /locus_tag="ACHK74_000710"
     CDS             11975..12739
                     /gene="yfeN"
                     /locus_tag="ACHK74_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFP0219576.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(12778..13704)
                     /gene="yfeR"
                     /locus_tag="ACHK74_000711"
     CDS             complement(12778..13704)
                     /gene="yfeR"
                     /locus_tag="ACHK74_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0219577.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            13794..14792
                     /gene="yfeH"
                     /locus_tag="ACHK74_000712"
     CDS             13794..14792
                     /gene="yfeH"
                     /locus_tag="ACHK74_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFP0219578.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSTDKA"
     gene            complement(14789..15007)
                     /gene="ypeB"
                     /locus_tag="ACHK74_000713"
     CDS             complement(14789..15007)
                     /gene="ypeB"
                     /locus_tag="ACHK74_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFP0219579.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(15009..17024)
                     /gene="ligA"
                     /locus_tag="ACHK74_000714"
     CDS             complement(15009..17024)
                     /gene="ligA"
                     /locus_tag="ACHK74_000714"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFP0219580.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(17095..18081)
                     /gene="zipA"
                     /locus_tag="ACHK74_000715"
     CDS             complement(17095..18081)
                     /gene="zipA"
                     /locus_tag="ACHK74_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFP0219581.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVTDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            18311..19072
                     /gene="cysZ"
                     /locus_tag="ACHK74_000716"
     CDS             18311..19072
                     /gene="cysZ"
                     /locus_tag="ACHK74_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFP0219582.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            19257..20228
                     /gene="cysK"
                     /locus_tag="ACHK74_000717"
     CDS             19257..20228
                     /gene="cysK"
                     /locus_tag="ACHK74_000717"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFP0219583.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            20612..20869
                     /gene="ptsH"
                     /locus_tag="ACHK74_000718"
     CDS             20612..20869
                     /gene="ptsH"
                     /locus_tag="ACHK74_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFP0219584.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            20914..22641
                     /gene="ptsI"
                     /locus_tag="ACHK74_000719"
     CDS             20914..22641
                     /gene="ptsI"
                     /locus_tag="ACHK74_000719"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFP0219585.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            22682..23191
                     /gene="crr"
                     /locus_tag="ACHK74_000720"
     CDS             22682..23191
                     /gene="crr"
                     /locus_tag="ACHK74_000720"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP0219586.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(23233..24084)
                     /gene="pdxK"
                     /locus_tag="ACHK74_000721"
                     /gene_synonym="thiJ"
     CDS             complement(23233..24084)
                     /gene="pdxK"
                     /locus_tag="ACHK74_000721"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFP0219587.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            24189..24557
                     /gene="yfeK"
                     /locus_tag="ACHK74_000722"
     CDS             24189..24557
                     /gene="yfeK"
                     /locus_tag="ACHK74_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFP0219588.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(24560..25471)
                     /gene="cysM"
                     /locus_tag="ACHK74_000723"
     CDS             complement(24560..25471)
                     /gene="cysM"
                     /locus_tag="ACHK74_000723"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFP0219589.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(25605..26702)
                     /gene="cysA"
                     /locus_tag="ACHK74_000724"
     CDS             complement(25605..26702)
                     /gene="cysA"
                     /locus_tag="ACHK74_000724"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFP0219590.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(26692..27567)
                     /gene="cysW"
                     /locus_tag="ACHK74_000725"
     CDS             complement(26692..27567)
                     /gene="cysW"
                     /locus_tag="ACHK74_000725"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFP0219591.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(27567..28400)
                     /gene="cysT"
                     /locus_tag="ACHK74_000726"
     CDS             complement(27567..28400)
                     /gene="cysT"
                     /locus_tag="ACHK74_000726"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFP0219592.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLILLFSINTLQSRFGRRVVG
                     H"
     gene            complement(28400..29416)
                     /gene="cysP"
                     /locus_tag="ACHK74_000727"
     CDS             complement(28400..29416)
                     /gene="cysP"
                     /locus_tag="ACHK74_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFP0219593.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(29587..30378)
                     /gene="ucpA"
                     /locus_tag="ACHK74_000728"
     CDS             complement(29587..30378)
                     /gene="ucpA"
                     /locus_tag="ACHK74_000728"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFP0219594.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(30507..31364)
                     /gene="murR"
                     /locus_tag="ACHK74_000729"
     CDS             complement(30507..31364)
                     /gene="murR"
                     /locus_tag="ACHK74_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFP0219595.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            31528..32424
                     /gene="murQ"
                     /locus_tag="ACHK74_000730"
     CDS             31528..32424
                     /gene="murQ"
                     /locus_tag="ACHK74_000730"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP0219596.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            32428..33852
                     /gene="murP"
                     /locus_tag="ACHK74_000731"
     CDS             32428..33852
                     /gene="murP"
                     /locus_tag="ACHK74_000731"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFP0219597.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            33857..35161
                     /gene="pbp4b"
                     /locus_tag="ACHK74_000732"
     CDS             33857..35161
                     /gene="pbp4b"
                     /locus_tag="ACHK74_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFP0219598.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(35219..36118)
                     /gene="yfeX"
                     /locus_tag="ACHK74_000733"
     CDS             complement(35219..36118)
                     /gene="yfeX"
                     /locus_tag="ACHK74_000733"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFP0219599.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(36214..36789)
                     /gene="yfeY"
                     /locus_tag="ACHK74_000734"
     CDS             complement(36214..36789)
                     /gene="yfeY"
                     /locus_tag="ACHK74_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFP0219600.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(36850..37299)
                     /gene="yfeZ"
                     /locus_tag="ACHK74_000735"
     CDS             complement(36850..37299)
                     /gene="yfeZ"
                     /locus_tag="ACHK74_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFP0219601.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(37286..37711)
                     /gene="ypeA"
                     /locus_tag="ACHK74_000736"
     CDS             complement(37286..37711)
                     /gene="ypeA"
                     /locus_tag="ACHK74_000736"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFP0219602.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            37925..38794
                     /gene="amiA"
                     /locus_tag="ACHK74_000737"
     CDS             37925..38794
                     /gene="amiA"
                     /locus_tag="ACHK74_000737"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFP0219603.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            38798..39697
                     /gene="hemF"
                     /locus_tag="ACHK74_000738"
     CDS             38798..39697
                     /gene="hemF"
                     /locus_tag="ACHK74_000738"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFP0219604.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(39703..40755)
                     /gene="eutR"
                     /locus_tag="ACHK74_000739"
     CDS             complement(39703..40755)
                     /gene="eutR"
                     /locus_tag="ACHK74_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFP0219605.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(40801..41301)
                     /gene="eutK"
                     /locus_tag="ACHK74_000740"
     CDS             complement(40801..41301)
                     /gene="eutK"
                     /locus_tag="ACHK74_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFP0219606.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(41314..41973)
                     /gene="eutL"
                     /locus_tag="ACHK74_000741"
     CDS             complement(41314..41973)
                     /gene="eutL"
                     /locus_tag="ACHK74_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFP0219607.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(41983..42870)
                     /gene="eutC"
                     /locus_tag="ACHK74_000742"
     CDS             complement(41983..42870)
                     /gene="eutC"
                     /locus_tag="ACHK74_000742"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFP0219608.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCV
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(42891..44252)
                     /gene="eutB"
                     /locus_tag="ACHK74_000743"
     CDS             complement(42891..44252)
                     /gene="eutB"
                     /locus_tag="ACHK74_000743"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFP0219609.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(44264..45667)
                     /gene="eutA"
                     /locus_tag="ACHK74_000744"
     CDS             complement(44264..45667)
                     /gene="eutA"
                     /locus_tag="ACHK74_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFP0219610.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLEEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(45664..46890)
                     /gene="eutH"
                     /locus_tag="ACHK74_000745"
     CDS             complement(45664..46890)
                     /gene="eutH"
                     /locus_tag="ACHK74_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFP0219611.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKDDATAAKTEAEAQS"
     gene            complement(47007..48194)
                     /gene="eutG"
                     /locus_tag="ACHK74_000746"
     CDS             complement(47007..48194)
                     /gene="eutG"
                     /locus_tag="ACHK74_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFP0219612.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWLCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDHD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(48184..49020)
                     /gene="eutJ"
                     /locus_tag="ACHK74_000747"
     CDS             complement(48184..49020)
                     /gene="eutJ"
                     /locus_tag="ACHK74_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFP0219613.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(49031..50434)
                     /gene="eutE"
                     /locus_tag="ACHK74_000748"
     CDS             complement(49031..50434)
                     /gene="eutE"
                     /locus_tag="ACHK74_000748"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFP0219614.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIDTAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(50446..50733)
                     /gene="eutN"
                     /locus_tag="ACHK74_000749"
     CDS             complement(50446..50733)
                     /gene="eutN"
                     /locus_tag="ACHK74_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFP0219615.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(50840..51133)
                     /gene="eutM"
                     /locus_tag="ACHK74_000750"
     CDS             complement(50840..51133)
                     /gene="eutM"
                     /locus_tag="ACHK74_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFP0219616.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(51172..52188)
                     /gene="pta"
                     /locus_tag="ACHK74_000751"
     CDS             complement(51172..52188)
                     /gene="pta"
                     /locus_tag="ACHK74_000751"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP0219617.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTKVNRESSLQ
                     TLVE"
     gene            complement(52185..52988)
                     /gene="eutT"
                     /locus_tag="ACHK74_000752"
     CDS             complement(52185..52988)
                     /gene="eutT"
                     /locus_tag="ACHK74_000752"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFP0219618.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(52985..53686)
                     /gene="eutQ"
                     /locus_tag="ACHK74_000753"
     CDS             complement(52985..53686)
                     /gene="eutQ"
                     /locus_tag="ACHK74_000753"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFP0219619.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(53661..54140)
                     /gene="eutP"
                     /locus_tag="ACHK74_000754"
     CDS             complement(53661..54140)
                     /gene="eutP"
                     /locus_tag="ACHK74_000754"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFP0219620.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(54153..54488)
                     /gene="eutS"
                     /locus_tag="ACHK74_000755"
     CDS             complement(54153..54488)
                     /gene="eutS"
                     /locus_tag="ACHK74_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFP0219621.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(54781..57060)
                     /gene="maeB"
                     /locus_tag="ACHK74_000756"
     CDS             complement(54781..57060)
                     /gene="maeB"
                     /locus_tag="ACHK74_000756"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFP0219622.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            57349..58299
                     /gene="tal"
                     /locus_tag="ACHK74_000757"
     CDS             57349..58299
                     /gene="tal"
                     /locus_tag="ACHK74_000757"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP0219623.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            58319..60322
                     /gene="tkt"
                     /locus_tag="ACHK74_000758"
     CDS             58319..60322
                     /gene="tkt"
                     /locus_tag="ACHK74_000758"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP0219624.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(60399..61442)
                     /gene="ypfG"
                     /locus_tag="ACHK74_000759"
     CDS             complement(60399..61442)
                     /gene="ypfG"
                     /locus_tag="ACHK74_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFP0219625.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(61568..62143)
                     /gene="nudK"
                     /locus_tag="ACHK74_000760"
     CDS             complement(61568..62143)
                     /gene="nudK"
                     /locus_tag="ACHK74_000760"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFP0219626.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(62211..64190)
                     /gene="aegA"
                     /locus_tag="ACHK74_000761"
     CDS             complement(62211..64190)
                     /gene="aegA"
                     /locus_tag="ACHK74_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFP0219627.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRHPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            64396..66096
                     /gene="narQ"
                     /locus_tag="ACHK74_000762"
     CDS             64396..66096
                     /gene="narQ"
                     /locus_tag="ACHK74_000762"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFP0219628.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            66260..69373
                     /gene="acrD"
                     /locus_tag="ACHK74_000763"
     CDS             66260..69373
                     /gene="acrD"
                     /locus_tag="ACHK74_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFP0219629.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(69472..69531)
                     /gene="ypfM"
                     /locus_tag="ACHK74_000764"
     CDS             complement(69472..69531)
                     /gene="ypfM"
                     /locus_tag="ACHK74_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFP0219630.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        16639 a        19086 c        17637 g        16416 t
ORIGIN      
        1 aggacagtcc ggcagcaacc agacttaacc caaagaccgt tgccgcagca tggcttaaca
       61 gcggttgcag cgtcagataa gcctcatcaa gatcggcaac accagtatga ccagaaaagt
      121 ggaacgccgc cgcagctgta gccatcatcg ccagattgac aaaaccggca atcgtcatgg
      181 cgatagccac atcccatttg gtggcggaat aacgttgttg acgcgaaccg ccatgtaaat
      241 gctgagtgag cgaagagtgc aaataaatca catgcggcat aatcgtcgcc cctaacacgc
      301 ctgctgccag gaagaccgct tccgaagtag gtaaactcgg gatcaccatt cctttaccca
      361 gctgcgccag gttaggctgg gaaaaaatca actcgacaat gtaagccgcg gcaacaaaca
      421 acagtaaccc accaatcact ttttccagcg gtttttgccc gcgacgttgc agcattaaaa
      481 tcaggaaagt cgcgatcccc gtcagcaccg cgccctgcaa caacgagaca ccaagaatga
      541 gtttaaaacc gatcgccgca ccaataaatt ccgccaggtc ggttgccatc gcaataattt
      601 ctgcctgaac ccaatagaac cacactacgg gacgcggata gtgatcgcga atctgctccg
      661 ccagattttt accggtggca atccctagtt tggcagagag gatctgaatc agcatcgcca
      721 tcaggttggc ccaaacgaca acccacagta gctgatagcc gaagctggca cccgcctgaa
      781 tattggtcgc aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac gcaggtccca
      841 ttaatgcgag cctcatcttg cgcgccgccc gtccgctgct actctcaacg cgatagttcg
      901 tcatcttgtg cctctaaaac atagcctttg ctatgtttca tgctatgcca aacgagaatg
      961 attatcaaat tcatttaaat gggttgtggt gatttctctg atagacctgg attatgacta
     1021 cgaaaagatt gatgacttga atgttatggt tatgtttaat gttagcacat ttacataact
     1081 ttcagcttcc atacacaaca tagcagaaat gtatgacaga tcactatttt tgaagcttgt
     1141 cacaggacgt cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt tacatagaat
     1201 gtgcacggaa atttaacctg cctcatattt ggagcaaata tggaccgcgt ccttcatttt
     1261 gtactggcac ttgccgttgt tgcgattctc gcactgctgg taagcagcga ccgcaaaaaa
     1321 attcgtatcc gttatgttat tcaactgctt gttatcgaag tgttactggc gtggttcttc
     1381 ctgaactccg acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt cgaaaaactg
     1441 ctcggatttg ccaacgaagg gactaacttc gtctttggta gcatgaatga tcaaggcctg
     1501 gcattcttct tcctgaaagt gctgtgccca atcgtcttta tctctgcact gatcggtatt
     1561 ctccagcaca ttcgcgtgtt gccggtgatt atccgcgcaa ttggtttcct gctctccaaa
     1621 gtcaacggca tgggcaaact ggaatccttt aacgccgtca gctccctgat tctgggtcag
     1681 tctgaaaact ttattgccta taaagatatc ctcggcaaaa tctcccgcaa tcgtatgtac
     1741 accatggcag caacggcgat gtccaccgta tctatgtcca tcgttggtgc atacatgacc
     1801 atgctggagc cgaaatatgt cgttgcggcg ctggtactga acatgttcag cacctttatc
     1861 gtgctgtcgc tgatcaaccc ttaccgtgtt gatgccagtg aagaaaacat tcagatgtcc
     1921 aacctgcacg aaggtcagag cttcttcgaa atgctgggtg aatacattct ggcaggtttc
     1981 aaggttgcca ttatcgttgc cgcgatgctg atcggcttta tcgccctgat cgccgcactg
     2041 aacgcactgt ttgctactgt gactggctgg tttggctaca gcatctcctt ccagggcatc
     2101 ctgggctaca tcttctatcc gattgcatgg gtgatgggtg ttccttccag tgaagcactg
     2161 caagtgggca gtatcatggc gaccaaactg gtttccaacg agttcgttgc gatgatggat
     2221 ctgcagaaaa ttgcttccac gctctctccg cgtgctgaag gcatcatctc tgtgttcctg
     2281 gtttccttcg ctaacttctc ttcaatcggg attatcgcag gtgcagttaa aggcctgaat
     2341 gaagagcaag gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg ctctaccctg
     2401 gtgagtgtgc tgtctgcgtc aatcgcagca ctggtgctgt aaaaccatac ataaaaaagc
     2461 cggggatcat tccccggctt ttttacgcct gttaatcagc taatggctgc gggcgaccaa
     2521 tcaaataccc ttgcagatat tgcaccccga gcttatgcaa tagcgcctgc tgctgttgcg
     2581 tctcgacaaa ctccgcgacc acactcaatg acttcgcttt cgccagatcg gtaattgatc
     2641 tcacaatcat cgcatccagc gtgttcgtga caatatcttt cacaaagacg ccatcaattt
     2701 tgatgatatc agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg gtgccaaagt
     2761 catcaatcgc aatccggaaa ccaaacttat gcagctgctc gatgttgtac atgctgcttt
     2821 ctgcgttaga aaacgcctgc tcctcggtga tctcaagaat gaccgactgc ggggagatgt
     2881 gataacgctt aaacagacga ataatccgcc cggcaatatt cttttgcagc agcgtgagcg
     2941 gcattaaatt gactgaaaag cgcggtcctt ttttgtcgca agggtgtgtt gccagccact
     3001 tcaacaagga ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac tgagcaataa
     3061 gggacagaaa cttatccggg gtcataatgc cgccgtcata tttcagtcgc gcgaggatct
     3121 catcataacc ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc aaatcaccct
     3181 gatctaacgc cgtgcgaatg gtattcagca atagcacctg tttagtggtc tgcccggaaa
     3241 ccatctcctc tctgctatcc agcgccagca catgatgatg tgcgcaggat tgctccgcca
     3301 gccagcttaa ctgccccaac aagggttgca gggtttcctg atttccatca aaacgccccc
     3361 aggcagcacc atagcccata tccagcccgg tattgttcca gtgaatttgc cgactattca
     3421 ggatgttaac catatgctgg agtcgccctt ccgtttccgg cccgctcagc actaacagca
     3481 gttcactacc cggcaattga tacaactttt cgttttcctg catcaacggc agcagcgtac
     3541 ggcaaattga gcggatacag tgaacgcgca tcattaagcc gtaatgacga ctcataaact
     3601 caagattatc aatgcgcagg cagcaaaaac tcttgcccgc ctcttgttcc ggcgcttgtt
     3661 ccaacgcacg aaagttgggt aggagcgtta acggatctgt cagcgcctgc aaatgccagc
     3721 ggcgattaag ccattcactg cgatgataaa tgcgcaccat atagagcagg caaacgctaa
     3781 aggaaatcag caccgcaaga ataaatgcca gcgaatattc ggtttccacc ccttgcaaaa
     3841 agttctggtt gtaattcagc aggcaaagcg ttgaaacagc ccaggtgaga tttaaaaacg
     3901 gatagcgaag cttaccgacc ccgagggtaa agatgatgaa gaaaacgggt accaagtaac
     3961 cggcaataaa gtcgttttca taaggtgtgc acaacagaag tagcagcacg cttaacgctg
     4021 ccagccagct taaggtaaat gcgcgtttct ctttgcccaa cgacggagcg atatccctgc
     4081 gccacaatat ctgcgcaaag tggggactta caatcatgcg agtgagatag tagaaaagca
     4141 tgttgtaaat cagcacagcg gtgaaaaggc ttagcaaatc aacgaccgtg aaaatggcat
     4201 ccgcatcgcc gaaaaaggtg gatatcttta gcgggaaatc aaagaagctc cccacaagat
     4261 acatgctgca tttgatgccg atcggcgtca ccaaaccaag ccagaacaga cgttgccaga
     4321 catatcggct ggtcaatcca taacgccaac gcgtccccag ctgccagcgt aatattgcgc
     4381 aggcacacaa cacgacaaac gtctggctac cgagcatgac cgcagtctgt aaaaaagaga
     4441 gatgaaagtt ccacagattg gtgcaaaaca tcccgcataa aacgggaacg acaccgcgcc
     4501 agccaaagat aaacaagatt gacagcatta cgcacagtgg catccacgcc agaaagatat
     4561 aactggaatg gataatggca agtggcgaaa taaaacggga tagctgaata agtaccacgg
     4621 tgagcgtaaa cgctagtgtg aatatcttga tattttttat caggttatgc tccacaaaca
     4681 tgagtacact actctcactg atcggggttt tcaggcacac aaataataag tttattcgca
     4741 tcgcgaagca aacaggggcc agcctgttgc cgatttttca tccaatgatg ctttcaccag
     4801 gcggcgctaa cccggacgta gactgtatta caaaagcggc aaaaagcaga gacaaaaaac
     4861 ccccgctttg cagcgagggt tggaaatttg gtggagctaa gcgggatcga accgctgacc
     4921 tcttgcatgc catgcaagcg ctctcccagc tgagctatag ccccacgatg cgtttacgta
     4981 ccaaatttgc tgggtgcaaa atttggtgga gctaagcggg atcgaaccgc tgacctcttg
     5041 catgccatgc aagcgctctc ccagctgagc tatagccccg tcacgtaaag cttgtcgagt
     5101 tgacgggcgg catcatatga attccgcccg aatgtgtcaa cggcaaattg caacgtgtag
     5161 tttcaatcgc tgaaaaatca ggcaaatgaa caattttgga aaaccgctcg cattcagtag
     5221 ttattcatgt cacggtttcc tgtaaagtgg tgttataaaa tgaactacta atagacccac
     5281 atacattcag ggaattgtta tgttcaagga gaggatgaca ccagatgaac ttgccagact
     5341 gaccggctat agccgccaga ccattaataa atgggtacgc aaggaaggct ggacaacgtc
     5401 accaaaacct ggcgtccagg gtggcaaagc ccgactggtt cacgtcaatg aacaggttcg
     5461 tgaatatatt cgcaatgctg aacgtccaga aggtcaggga gaagcgcctg ccctttctgg
     5521 tgatgcgccg cttgaagtat tgctggtgac actggcgaaa gagatgacgc cagttgaaca
     5581 aaaacagttt acatccttgc ttctgcggga agggattatc ggattgttac aacgcttagg
     5641 gattcgcgat agcaaataat atgaaaagat tacgcaacaa aatgaccacc gaagaactgg
     5701 ctgaatgcct cggtgtggct aaacaaactg ttaaccgttg gatcagagaa aaaggctgga
     5761 aaacggaaaa atttcccggc gtgaaaggcg gtcgtgccag acttattctg gtcgatacgc
     5821 aagtttgcga gtttattcag aacacgccag ccttccataa cacaccaatg ttgatggaag
     5881 ctgaagagag aattgctgaa tacgctcctg gagcacgcgc cccggcgtat cgacaaatca
     5941 tcaacgcgat agataatatg acagacattg agcaggaaaa agtcgcgcaa tttttgtcac
     6001 gtgaaggaat tcgcaatttc ctcgcccgtc tcgatattga cgaatcagca taaataaaaa
     6061 acggcaggat attatctcct gccgtttatc tttttacacg ctaattactg ctgattttcg
     6121 cgttcagcaa taaaatccag cgctttgttg atacgctcga tactgcgggt cttaccaatt
     6181 gcgtgaacgg taacatccag tgctggagac tgccccgcac cggttacggc gacacgcagc
     6241 ggcataccaa ctttacccat acccacttcc agctcatccg ccgtcgcctg aatagcgtga
     6301 tgaacgtttt cagcggtcca gtcagtaatc gcggccagtt tgtcacgaac cacttccagc
     6361 ggctgacgcg ctaccggacg cagatgtttt ttcgccgcat cggcatcgaa ctcagcaaaa
     6421 tcttcgtaga aataacggca gctctgtgcc atctctttca gcgtcttgca gcgttcgccc
     6481 agcagtttca ccagatcagc cagctgcggg ccgttacggg tatcgatatt ttcctgctca
     6541 atgtgccact gtaagtgagt agcaacatac tccggcggca gcgcgttaat gtagtgatgg
     6601 ttcagccaca gcagcttgtc ggtgttgaac gcactggcag atttgctgac ggcattcaaa
     6661 gtgaagtatt tgatcatctc ttcacgagtg aagatttcct gatcgccgtg ggaccagccc
     6721 agacgcacca gatagttcag cagtgcttct ggcaaataac cgtcatcacg atactgcatt
     6781 acgctgactg ccccgtgacg tttggacagt tttttaccgt catcgccatt gatcatagaa
     6841 acgtgcgcgt aaaccggcac cggcgctttc agggccttaa gaatgttgat ctggcgcggc
     6901 gtgttgttga tatggtcttc gccacggata acgtgggtga tttccatatc ccagtcatcg
     6961 acaaccacac agaagttata ggttggggaa ccatcggtac ggcggataat cagatcatcc
     7021 agctcctggt tgctgaactc gatcggacca cggatctgat cgtcaaaaac aacagaacct
     7081 tcctgcggat tagcaaaacg cacgacacac ggttcatcat cagcatgatg ctcatggctg
     7141 tggcggcagc gaccgtcata acgcggcttc tcacctttcg ccatttgctc ttcgcgcagc
     7201 gcttccaggc gctctttaga gcaatagcat ttataagcag tgccctcttc cagcatctga
     7261 tcgatcaccg cattgtagcg atcaaaacgt ttggtctggt agtacggacc ttcatcccac
     7321 tccaggctca gccagttcat gccatccata atggcttcga tagcttccgg cgtggaacgc
     7381 tcaagatcgg tgtcttcaat acgcagcacg aactcaccgc cgtggttacg tgcaaaaagc
     7441 caggagtaaa gagcagtacg cgcgccgcca acgtgcagat agcctgttgg gcttggcgcg
     7501 aagcgagttt tgattttcat gaaatggcct tacgtttaga aagatgccga caaccggcaa
     7561 atcctggaaa aattgagtgg gcgatattct atcactcccg cctgattcct caatgtagtt
     7621 cgggtttcta ccttacgcat tattgttttc gttgacaaat tgcgcaacac ggtctgtttt
     7681 gcgatcatct tgcttaaatt tacgacgaac gaacaatttc tttagaaaat gcgttgactc
     7741 attttgaact ctccctataa tgcgactcca cacagcgggg gtgattagct cagctgggag
     7801 agcacctccc ttacaaggag ggggtcggcg gttcgatccc gtcatcaccc accaactact
     7861 ttatgtagtc tccgccgtgt agcaagaaat tgagaagtgg gtgattagct cagctgggag
     7921 agcacctccc ttacaaggag ggggtcggcg gttcgatccc gtcatcaccc accactttct
     7981 cgccagctaa atttcttgta aaaatgtgaa gtacctaagt gggtgattag ctcagctggg
     8041 agagcacctc ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccacttc
     8101 gggtcgttag ctcagttggt agagcagttg acttttaatc aattggtcgc aggttcgaat
     8161 cctgcacgac ccaccaatgt aaaaaagcgc cctaaaggcg cttttttgct atctgcgata
     8221 ctcaaagatt cgaacctgca gcaggtttga gttgagcgca gcgaaacaac ggagccgctc
     8281 gcggcgacgg cccgaagggc gagcgaagcg agtcatcctg cacgacccac caatgtaaaa
     8341 aagcgcccta aaggcgcttt tttgctatct gcgatttgcg aaattgcctg atgcgcttca
     8401 cttagcagac gactatttcc ggcaattcct gtctcctcac ctactgtgtc aatgcagcca
     8461 acagcttaac catcgcgggc gtcacctgct gtgtttcata aacaatatat aaatctgcag
     8521 ggatgcgctg tttgagcgga cggaaaatga cacctggcca gttcatttgt gcgtagctgt
     8581 ccgctatcaa tgtgatacca atacccatac tgaccatggc gagtaccgtt tgcggttcat
     8641 taacttcgcg aataacaacc ggtgaaaatc ccacctgctg gcaaactcgc tgcaaaaaat
     8701 cccagtcagt gtacacgggc ggcattgtaa caaaatactc gtcacgtagc gcttccagcg
     8761 gaacggtgga aaatgatgag agatgatgct cttcaggcat cgccaccaga aacgccgatt
     8821 catgcaaccg taagctggta aaacctgtcg gtggttctgt cgccattcgc cagatcccgg
     8881 catcgagttc acgtcgttcc agcagggcca tttgcatcgc gggcatcttt tcgcgaaaaa
     8941 gaacttcaac gttaggattt tccctgagga atcgccgcat aaccgggcgc atccgtcccc
     9001 acatagccgt tcccactacg ccgagttcaa tccgccctgc ttctccccga cctatttgtt
     9061 caacccgagc caatacatta ttagcattca ccagcaatcg acgcgattct tccatcaaga
     9121 ttttgcccgc gtgtgtcagt acgacgctgc gcgaatggcg aataaaaagc tgcgtgccga
     9181 gttgattttc cagctcttta atatgaatac tgagcggagg ctgagacata tttaaacgcg
     9241 ctgctgcgcg gccaaaatgc aactcttccg ctacggcaag aaaataacgg agcaacttaa
     9301 gatctgttct gtatacgcgt tccataatta agtgctccat ataataaata catcgtatta
     9361 aattatcatg gcataaagta tttcaccacc agatatccga catacataaa cacaatggat
     9421 aatatacctg gtaatacatt tggtacagga ataggcaagc gcacgattgt aaacaaagca
     9481 ccaatgccaa aaccaacgcc ggaagaaaat aaaatatctt tcactaatta acctttatca
     9541 taaaagcagc tctgaagagc agagctgcga atccttttaa tgagccaccg ctcgatgctt
     9601 tatcttttca gggtcatgat tatatttaaa cccaaagaaa aatatcactg cgagaaaaag
     9661 agcatatcct gcaaacacca gccagatagt ttgccagtct tttacgccat ccaccgaaaa
     9721 gtaatctact gccatgccac tcagaatcga gccaacccat gcgccgacac catttaccat
     9781 ggtcataaag agcccctgcg cgctggcacg aatgctggaa tcaacttcct gttcgacaaa
     9841 taccgaacca gaaatattga agaaatcgaa tgcacagcca taaacaatca tcgacagcag
     9901 cagcaaaata aatccggttg ttgacggatc gccataggcg aagaagccaa agcgcagcgt
     9961 ccaggccacc atactcatca gcatgacggc tttaatgcca aatcgcttta aaaagaatgg
    10021 gatagtcagt ataaagccca cttctgccat ctgtgaaact gacagtaaaa tggagggata
    10081 tttcaccaca aaactgtcag caaactccgg gttacgggcg aaatcatgta ggaacggatt
    10141 accaaaaacg ttggtaattt gcagtaccgc acccagcatc atggcaaaga ggaaaaagat
    10201 ggccatgcgt ggatttttaa acagcacgaa ggcatccaga cccagcttgc tggcaagcga
    10261 tgtggtcgct tttttctccg caaccggaat cttcggcaaa gtcagcgcat aagccgacag
    10321 cagcaatgac gcaccggacg cgatatacag ctgcagacta ctcaattcca gatgcagcag
    10381 gcttactgcc cacatcgcga caatgaaccc caccgtacca aaaacgcgaa tgggcgggaa
    10441 agcggtcacc gggtcaagcc ctgcctgggc aagacaggaa taagagacgc tgttcgataa
    10501 cgcaatagtc ggcataaacg ccatcgcatt gactaacatc acccaaaaca tcatatccgg
    10561 atcagttacg gatgccgcat aaaaaagtac gcccgcacac accaggtgac acagcatgta
    10621 tgcacgttct gcgcgcagcc atttgtctgc gatgatcccc attataccag gcataataat
    10681 cgcggcgatc cctttggaac tgtaaaccat gccaacatta gcgccggtga aatgaagggt
    10741 attaatcatg taagagccga gggtaaccag ccagctcccc cagataaaat attgcaaaaa
    10801 ggacattacc tttaagcgca tcgcgatgct cataatcgtt tccttgtcgc atcatttggc
    10861 ctcacccgcg aggccgtttc ttattgtttt tctcaggcaa ttttgcgcag aaagccacaa
    10921 ataaggttga tgaagttctg cttagagagc tccgcagccg ccagcgtttg cgcatgtgac
    10981 aatttcacat cgcttaaacc ttccgccata ttggtaatcg cagagaccgc aacgacttta
    11041 aggtcgcaat ggcgagctga aataacctca ggcaccacag acataccaac aacatcccca
    11101 ccaataattt gcatcatgcg aatttccgcc gcagtctcga aattcggccc cggatacgag
    11161 acgaacacgc cctccgtcag agggaaaccc tcttctttcg ccactttttg taacagtgcg
    11221 cggtattccg catcgtaggc atttgccagt gagaagaagc gctctccaaa acgatcatcg
    11281 ttaagaccca ccatcggcgt accgggcatg gtgttgatat gatctttcaa tgcgaccagg
    11341 ctgcctgccc ccacttccgg acgcagcgag cctgccgcat tggtgcagaa cagtagctcg
    11401 cagcccagca gcttaaaggt acgaattgcg tcagtcataa tggtcattcc acgaccttcg
    11461 tagaaatgtc cgcgaccttt catacatacc accggtacgc cctggagatg gcccagcact
    11521 aactcccccg catgaccatg taccgtactt accgggaagc ccggcagctt ttcgtaggaa
    11581 attgcgacag cgttctcaat ctgatcggcc agcgcgccga gcccggaacc taaaataaag
    11641 gccactcgtg gcgtgaaatc aggtttataa gtcttgataa tatctatgca aaacagcggg
    11701 ttatgagaaa attgaacctg agacatacat atcctttttc tgtagggtgg aatctaacgc
    11761 ctttttatag cgaggttgtc ccggctctta ccaatacact tttctcacgc attcaatagc
    11821 gattcgatat cgatggattt atatcttttc tctattaatc cacggtaaaa actgtattgg
    11881 cgatattaat tcctttgcat taacaatatg tcgtttttag caaaccactt tattaagcaa
    11941 tctactaata acgatcggca tagggaatta tgctatgaaa aaacatcttt taactctgac
    12001 actttcctct atattagcga tacctgttgt atctcatgca gaatttaaag gcggttttgc
    12061 agatatcggc gtgcattatc tggactggac cagccggact accgaaaaat cgtcgaccaa
    12121 atcacacaaa gatgattttg gttatcttga atttgaaggt ggcgctaact ttagttgggg
    12181 agaaatgtac gggttcttcg actgggaaaa cttctataac ggtcgccata ataaaccagg
    12241 tagcgagcag cgttatacct ttaaaaatac caaccgtatt tacctgggtg acaccggatt
    12301 taatctctac ttgcatgcgt atggcaccta cggttctgcg aatcgcgtga atttccacga
    12361 cgatatgttc ctgtacggta tcggctacaa tttcaccggc agcggttggt ggttcaaacc
    12421 gttctttgct aagcgttata cagatcaaac ctattacact ggcgacaacg gctatgtcgc
    12481 cggctgggtt gcaggttaca actttatgct gggcagcgag aaattcactc ttaccaactg
    12541 gaacgagtac gagtttgacc gtgacgctac ctatgcagcg ggtaatggcg gtaaagaagg
    12601 gctgaatggc gcagttgcac tgtggtggaa tgcaacatca cacattacta cggggattca
    12661 gtatcgatat gcggatgaca aactgggcga agatttttac caggatgcga taatctactc
    12721 catcaaattt aacttctgat tgattactgc cggggctccc ggcagtatga attcactcta
    12781 tatctgatac aacggatccc cttcccgacc agccattagc gcattgccct ggtctcgtac
    12841 cacgtcccat aacgcttcag cggcggtgga aagtgaacgg ttcttcctgc gcaccagcat
    12901 caactggcgt tcaacaaccg gcgtaatgcg ctttaccacc agtgggctac cttcaggcaa
    12961 tggtagcgcc agtgcgggaa gaatactgat gccgatgcct gcggctacca tcgggaacag
    13021 cgtcgccgga tgtccaatct cctgcacaat attcgcctga ataccattgc gcgccagcgc
    13081 tgcgtcaatc agcggtcggc tgcctgacgc gtaatcctgc aacaccaatt tcgctccctg
    13141 taatgcctgc cagggtacat aatcttcgac ggccaaagcg ctatcgcgat ggcaaagcag
    13201 aaagaaaggc tcggaaagaa tcgcttcaca ttgcagatcg cccactgggc ctggatcgat
    13261 gacgatgcca aagtcgacat ctccctggcg aatactttcc atcacccact gctgcggacg
    13321 atcgtgcagg acaaactgaa tatctggata gcggcgatgg ctttcagcaa tgcattgcgg
    13381 aataagatgc gcggaaatgg tctggctggc agcgacccga acttttccgc tcagttgttg
    13441 ccccatacgc ccggtatcgc gcaacgtgct gttcagttca tccagcagtc gctcaagacg
    13501 caaagccagc tgctgccctg catctgtaag caccacttca cgcgtggttc tgtccagcag
    13561 gcgaacacca gtatgatttt ccagttcctt cacactgtga ctcactgccg actggctcag
    13621 gccgatacgc tctcctgcac gactaaaact tttctcctgc gctactgtga cgaaaacctt
    13681 taattgtttt aaagaataat tcatctgttt tcttcatgaa tggatgcaat aaatcaattt
    13741 tatttctcaa actggaagaa gcacaataga accatcgatc atctggagtc tttatgaaac
    13801 tttttcgtat cctcgatcct ttcaccttaa ccctgatcac ggtggtgttg ctggcctctt
    13861 tctttccggc cagaggcgat ttcgtcccct tctttgaaaa tctgaccacc gcagcgattg
    13921 ccctgctgtt ctttatgcac ggcgcgaagt tgtcgcgtga ggcgattatt gctggcggtg
    13981 gtcactggcg actgcatttg tgggtaatgt gcagcacctt cgtgctgttt ccgattctgg
    14041 gtgtactgtt tgcctggtgg aaaccggtaa atgtcgaccc gatgctctac tccggttttc
    14101 tctacttgtg cattctcccg gctaccgtgc agtctgcaat cgccttcacg tcaatggcgg
    14161 gcggtaacgt cgcggcggcg gtttgttctg cgtcagcatc cagcctgctg gggattttcc
    14221 tttcaccatt gctggttggt ctggtgatga atgttcacgg tgcagggggc agccttgagc
    14281 aggtcggtaa aattatgctg caactgctgc tgccgtttgt gttggggcat ctttcccggc
    14341 cgtggattgg tgactgggtg tcgcgcaata aaaaatggat tgcgaaaact gaccagacgt
    14401 ccattctgtt ggtggtttat acagcgttca gcgaagccgt cgttaatggt atctggcata
    14461 aagttggctg gggatcattg ctgtttatcg tggtggtcag ctgcgttctt ctggctatcg
    14521 tgattgtagt taacgtcttt atggcacgcc gactgggctt caataaggca gatgaaatta
    14581 ctatcgtctt ttgtggttcg aaaaagagtc tggcaaatgg catcccgatg gcaaacattc
    14641 tgttccccac atcggtgatc ggtatgatgg tgctgcccct gatgattttc catcagatcc
    14701 aattgatggt ctgtgcggtg ctggcgcgtc gatacaaacg ccagaccgaa cagttacagg
    14761 cgcagcagga aagcagcacc gataaagctt aaagcggacg cttcaggggc tggaccagtt
    14821 gcgtcagccc ctcggtttta atcagcagcg tgatttgcat tagctcaccg agcttccctg
    14881 ccgggaactc atctttgcgg gcaaaccaca gcagatactc ctccggcagg tcgattaagc
    14941 gacgcccttt gtatttgcca aacggcatta tcgtattggc tatttcaatc agctgctctt
    15001 tttccatctc agctacccag caaacgcagc atttccgctt cgtcgatgac ttcaatgccc
    15061 agttcctgcg ccttcgccag tttagatcct gcagcttcac ccgctatcac cagatcggtt
    15121 ttcttcgaca cgctgcccgc gactttcgcg cccagttcga ccagtcgagc tttagcgtca
    15181 tcacgtgaca tctgacttaa gctgcccgta agcaccacgg ttttaccagc aaacgggctg
    15241 tcaatctctt ccgcgttgat aacgatcggc gcaggccagt ggacaccttc cgccaacagc
    15301 tcgctgataa cattgcggtt gctttcttcg gcaaagaagt tgtgaacgtg ggatgcaacg
    15361 acaatgccaa catcaggcac cttttgcagc tcttcaatcg aagcggcttc cagcgcttcc
    15421 agcgtgccga aatatgccgc cagacctgct gcggtggcct cgccgacttc acggatgcca
    15481 agtgcataga ggaagcgagc aaaggtggtt tctttcgctt tttctagcgc gttaaccacg
    15541 ttttgtgccg attttggccc catacgctcc agtccggtca gtttgcctgc ggtgagtttg
    15601 aacagatctg ccggagtatg gacatattct ttttcaacca gctgatcgat gattttgtcg
    15661 cccattccgt caacatccat cgcacggcgg gaaacaaagt gtttcagcga ctctttacgc
    15721 tgcgcaccgc aaatcaggcc accggtacag cgggcaaccg cttcaccttc cacacgctca
    15781 acgtcagaac cacataccgg acaatgcgtt gggaatacaa cctcacgggt atcttccggg
    15841 cgttcagaaa gcacgacgtt aaccacctgc gggatcacgt cgccagcgcg gcgaatcacc
    15901 actttatcgc caatgcgtaa accaagacgt tcgatttcat ccgcattgtg taaggttgcg
    15961 ttactcacca gcacgccagc aacatggaca ggttccagac gcgcaacagg cgtaatcgcg
    16021 ccagtacgcc caacctgaaa ctcgacgtca cgcacaaagg tcatctgctc ctgcgccggg
    16081 aatttaaacg ctaccgccca gcgcggggca cgcgcgacaa agccaagctg ctcctgctgt
    16141 tccagtgagt tgaccttaat caccacgccg tcgatatcaa agcccagcgt cgggcggtct
    16201 tcttccactt tgtgatagaa cgccagcact tcttccgccg attcacaaag cgttacccga
    16261 tcgctgaccg gcaaccccca ctttttaaat tgcagtaaac ggccaagatg agtatccggc
    16321 agctcgccac cttccagaac accaacgcca tagcagaaaa aagtgagcgg tcgcttcgct
    16381 gtaatacgcg gatcaagctg acgcagtgaa ccagctgccg cattacgtgg gttagcaaaa
    16441 actttcccgc ccgtgcgtcg cgcatcttcg ttaatctttt cgaaccccgc ctgcggcagg
    16501 aacacttcac cacgcacttc cagacgcgcc gggatattct ctccgtgcag cttcagcgga
    16561 atagcgcgaa tagtacgcac attagacgtg atatcttccc cggtggtgcc atcgccacgg
    16621 gtcgcggcac tgactaaaac gccattttca tacagaatac tgacggcaag gccatccagc
    16681 ttcagctcac agcaccaggt gactttctcg ttgtttttca gacggtcctg cacacgtttg
    16741 ttgaaagcaa gaaagctttc ttcatcaaaa acgttatcca gtgacagcat tggtacttca
    16801 tggcgtatct ggctgaaagc cgccagcggc gcagcgccta cacgttgggt aggcgaatca
    16861 ggcgtaatca gttctggatg tttggtttcc agctcgcgca gttcgcgcat cagcctgtcg
    16921 tattcagcgt cgggaatttc cggcgcatcc atcacatgat aaagatattc atgatggcga
    16981 agcgtcgttc gcagttctgt cagttgttgt tcgattgatt ccatatcgca ccatcaatgc
    17041 taaaaacccc cgacaagcgg gggttcgaag aggagttaat ttgccttaag tgtatcaggc
    17101 gttggcgtct ttgacttcgc ggatgatgtc ctggtactcg cgcaatttct gcggagtcat
    17161 catacggcgc tgatcgtcaa gcacgacacc gcccacttca tcggcaatat gctgcgcaga
    17221 ttgcagcatc agcttgaagt tctgcagctc gtcaccgtaa gacggtacct gcataaagat
    17281 ggtgacaccc ggagtagtga aatccttcat ttcaggatca aaggttcccg gtttcaccat
    17341 attcgccagg ctgaataacg ccgggccgct gccatccggg ctaagatgac gatggtaaat
    17401 attcatatcg ccaaaaatga agcccgcttg ttgaatgctg ttaagaagca gttcaccgtt
    17461 tagctcgcta ccgtgatgcg ccgcgacgtt catgataatc accgcttctt tgcgcttcgg
    17521 tttatccgta actggagccg gttccgctac aggctcaggc tgtggtgccg ctacgggttc
    17581 tgcaggctgg aaagcctgtt gtgccggttg cggtgctgaa tgcacaggct gcggcgctgg
    17641 cgcgacctgt ggcgaaactg gctgctgcaa cggctgttca ggctgcggct gataggcagg
    17701 ctgctgcacc ggctgcgctg gacgcggagc atgttgcggc ggtacctgcg cttcaggcgg
    17761 ctgctggacc ggttgacgcg gctgcgcaga cgcataaggc ggttggtact ggtgttgcgg
    17821 cgacggacga gcagcctcat gctcctgagc gttagccggg gcatgattca cgcggtgaac
    17881 acgaacctca ccaacgccct catcatcttc gacatcctcg tcataagaat cgtcgtcacg
    17941 ttttgacttc attcgtttta atggccgatc gcggaacata gaagatcgtt ctttacggct
    18001 ggtccagaaa ccatgtacca gtaaagcgat tatggcgatc gcgccaacaa tgattaatat
    18061 cagacgcaaa tcctgcatca ttatattctc tgttgttcta acaccttgcc accacggcaa
    18121 acatttactc actaagagta tttgccgatt acctcaagtg caagtgcact attaactttc
    18181 acagcacaaa gatagatgaa atcgtgcttt ttgctgtttt ttcgaacata tcctaactgt
    18241 ccattgcgca attacccggt aaaatacgca gaattttcct gggattggtc aaaaggagct
    18301 catcctgatt atggtttcat cattcacatc tgccccacgc agcggttttt actattttgc
    18361 gcaaggctgg aagctcgtct cgcaacctgg gattcggcgt ttcgttattt taccgctgct
    18421 ggtcaatatt ttgttgatgg ggggcgcatt ctggtggctc tttacacagc tcgatgtctg
    18481 gatcccgact ctcatgagtt acgttccgga ctggctacaa tggctgagtt atctgttgtg
    18541 gcctctggct gtcatctctg tgctgttagt gtttggctat ttcttctcca cgattgctaa
    18601 ctggattgcc gctccgttta acggtttatt ggctgaacaa ctggaagcac gattgactgg
    18661 cgctacaccg ccagataccg ggattttcgg tatcatgaaa gatgtgccgc gaatcatgaa
    18721 acgcgaatgg caaaaatttg cctggtatct gccgcgcgca attgtattgc taattcttta
    18781 cttaatccct ggtattgggc aaaccgtcgc gccggtactg tggttcctgt ttagcgcctg
    18841 gatgttagcc atccagtatt gcgattaccc cttcgataac cacaaagtgc cgtttaaaga
    18901 gatgcgcacc gccctgcgca cacgcaaaat caccaatatg cagtttggtg ctttaaccag
    18961 cctgtttacg atgatcccgc tgcttaatct gttcatcatg cccgttgccg tttgtggcgc
    19021 gacggcgatg tgggtcgatt gctatcgcga taaacacgcg atgtggcggt aacaatctac
    19081 cggttatttt gtaaaccgtt tgtgtgaaac aggggtggct tatgccgccc cttattccat
    19141 cttgcatgtc attatttccc ttctgtatat agatatgcta aatccttact tccgcatatt
    19201 ctctgagcgg gtatgctacc tgttgtatcc caatttcata cagttaagga caggccatga
    19261 gtaagatttt tgaagataac tcgctgacta tcggtcacac gccgctggtt cgcctgaatc
    19321 gcatcggtaa cggacgcatt ctggcgaagg tggaatctcg taaccccagc ttcagcgtta
    19381 agtgccgtat cggtgccaac atgatttggg atgccgaaaa gcgcggcgtg ctgaaaccag
    19441 gcgttgaact ggttgaacca accagcggta ataccgggat tgcactggcc tatgtagctg
    19501 ccgctcgcgg ttacaaactc accctgacca tgccagaaac catgagtatt gaacgccgca
    19561 agctgctgaa agcgttaggt gcaaacctgg tgctgacgga aggtgctaaa ggcatgaaag
    19621 gcgcaatcca aaaagcagaa gaaattgtcg ccagcaatcc agagaaatac ctgctgctgc
    19681 aacaattcag caatccggca aaccctgaaa ttcacgaaaa aaccaccggt ccggagatct
    19741 gggaagatac cgacggtcag gttgatgtat ttattgctgg cgttgggact ggcggtacgc
    19801 ttactggcgt cagccgctac attaaaggca ccaaaggcaa gaccgatctt atctctgtcg
    19861 ccgttgagcc aaccgattct ccagttatcg cccaggcact ggctggtgaa gagattaaac
    19921 ctggcccgca taaaattcag ggtattggcg ctggttttat cccagctaac ctcgatctca
    19981 agctggtcga taaagtcatt ggcatcacca atgaagaagc gatttctacc gcgcgtcgtc
    20041 tgatggaaga agaaggtatt cttgcaggta tctcttctgg agcagctgtt gctgcggcgt
    20101 tgaaactaca agaagatgaa agctttacca acaagaatat tgtggttatt ctaccgtcat
    20161 cgggtgagcg ttatttaagc accgcattgt ttgccgatct cttcactgag aaagaactgc
    20221 aacagtaatg ccagcttgtt aaaaatgcgt aaaaaagcac ctttttaggt gcttttttgt
    20281 ggcctgcttc aaactttcgc ccctcctggc attgattcag cctgtcggaa ctggtattta
    20341 accagactaa ttattttgat gcgcgaaatt aatcgttaca ggaaaagcca aagctgaatc
    20401 gattttatga tttggttcaa ttcttccttt agcggcataa tgtttaatga cgtacgaaac
    20461 gtcagcggtc aacacccgcc agcaatggac tgtattgcgc tcttcgtgcg tcgcgtctgt
    20521 taaaaactgg cgctaacaat acaggctaaa gtcgaaccgc caggctagac tttagttcca
    20581 caacactaaa cctataagtt ggggaaatac aatgttccag caagaagtta ccattaccgc
    20641 tccgaacggt ctgcacaccc gccctgctgc ccagtttgta aaagaagcta agggcttcac
    20701 ttctgaaatt actgtgactt ccaacggcaa aagcgccagc gcgaaaagcc tgtttaaact
    20761 gcagactctg ggcctgactc aaggtaccgt tgtgactatc tccgcagaag gcgaagacga
    20821 gcagaaagcg gttgaacatc tggttaaact gatggcggaa ctcgagtaat ttcccgggtt
    20881 cttttaaaaa tcagtcacaa gtaaggtagg gttatgattt caggcatttt agcatccccg
    20941 ggtatcgctt tcggtaaagc tctgcttctg aaagaagacg aaattgtcat tgaccggaaa
    21001 aaaatttctg ccgaccaggt tgatcaggaa gttgaacgtt ttctgagcgg tcgtgccaag
    21061 gcatcagccc agctggaaac gatcaaaacg aaagctggtg aaacgttcgg tgaagaaaaa
    21121 gaagccatct ttgaagggca tattatgctg ctcgaagatg aggagctgga gcaggaaatc
    21181 atagccctga ttaaagataa gcacatgaca gctgacgcag ctgctcatga agttatcgaa
    21241 ggtcaggctt ctgccctgga agagctggat gatgaatacc tgaaagaacg tgcggctgac
    21301 gtacgtgata tcggtaagcg cctgctgcgc aacatcctgg gcctgaagat tatcgacctg
    21361 agcgccattc aggatgaagt cattctggtt gccgctgacc tgacgccgtc cgaaaccgca
    21421 cagctgaacc tgaagaaggt gctgggtttc atcaccgacg cgggtggccg tacttcccac
    21481 acctctatca tggcgcgttc tctggaactg cctgctatcg tgggtaccgg tagcgtcacc
    21541 tctcaggtga aaaatgacga ctatctgatt ctggatgccg taaataatca ggtttacgtc
    21601 aatccaacca acgaagttat tgataaaatg cgcgctgttc aggagcaagt ggcttctgaa
    21661 aaagcagagc ttgctaaact gaaagatctg ccagctatta cgctggacgg tcaccaggta
    21721 gaagtgtgcg ctaacattgg tacggttcgt gacgttgaag gtgcagagcg taacggcgct
    21781 gaaggcgttg gtctgtatcg tactgagttc ctgttcatgg accgcgacgc gctgcccact
    21841 gaagaagaac agtttgctgc ttacaaagca gtggctgaag cgtgtggctc gcaggcggtt
    21901 atcgttcgta ccatggacat cggcggcgac aaagagctgc catacatgaa cttcccgaaa
    21961 gaagagaacc cgttcctcgg ctggcgcgct atccgtatcg cgatggatcg taaagagatc
    22021 ctgcgcgatc agctccgcgc tatcctgcgt gcctcggctt tcggtaaatt gcgcattatg
    22081 ttcccgatga tcatctctgt tgaagaagtg cgtgcactgc gcaaagagat cgaaatctac
    22141 aaacaggaac tgcgcgacga aggtaaagcg tttgacgagt caattgaaat cggcgtaatg
    22201 gtggaaacac cggctgccgc aacaattgca cgtcatttag ccaaagaagt tgatttcttt
    22261 agtatcggca ccaatgattt aacgcagtac actctggcag ttgaccgtgg taatgatatg
    22321 atttcacacc tttaccaacc aatgtcaccg tccgtgctga acttgatcaa gcaagttatt
    22381 gatgcttctc atgctgaagg caaatggact ggcatgtgtg gtgagcttgc tggcgatgaa
    22441 cgtgctacac ttctgttgct ggggatgggt ctggacgaat tctctatgag cgccatttct
    22501 atcccgcgca ttaagaagat tatccgtaac acgaacttcg aagatgcgaa ggtgttagca
    22561 gagcaggctc ttgctcaacc gacaacggac gagttaatga cgctggttaa caagttcatt
    22621 gaagaaaaaa caatctgcta atccacgaga tgcggcccaa tttactgctt aggagaagat
    22681 catgggtttg ttcgataaac tgaaatctct ggtttccgac gacaagaagg ataccggaac
    22741 tattgagatc attgctccgc tctctggcga gatcgtcaat atcgaagacg tgccggatgt
    22801 cgtttttgcg gaaaaaatcg ttggtgatgg tattgctatc aaaccaacgg gtaacaaaat
    22861 ggtcgcgcct gtagacggta ccattggtaa aatctttgaa accaatcacg cgttctctat
    22921 cgaatctgat agcggcgttg aactgttcgt ccacttcggt atcgacaccg ttgaactgaa
    22981 aggcgaaggc ttcaagcgta ttgctgaaga aggtcagcgc gtgaaagttg gcgatactgt
    23041 cattgaattt gatctgccgc tactggaaga gaaagccaag tctaccctga ctccggttgt
    23101 tatctccaac atggacgaaa tcaaagaact gatcaaactg tccggtagcg taaccgtggg
    23161 tgaaaccccg gttatccgca tcaagaagta attcttgccg cagtgaaaaa tggcgcccat
    23221 cggcgccatt ttttatgctt ccgccagcgg cggcaaaatc aattcatcgc tctcatgctg
    23281 ctgggtgtag cgcatcactt ccagtacgcg caaccccgca cggtgcaccg catcgtttaa
    23341 cgccttccct ttcagcaaac cactgataag ctgagcacaa aacaggtcgc cagtcccttt
    23401 caggtcggtt tttacccgtg aatgggaaat gacattcacg ctgtcggcac tgaccaccac
    23461 cacctgcatc tcctgatttt cttcattacc ggaggcgctg gtaatcacca cccattttaa
    23521 tgtgtctgaa agcagacttt ttgcggcagc aatggcactg tcgagatcgc ggcaattttt
    23581 accggtcagg atttccagct caaagatatt gggggtaatt ccctgcgcca gcggcagtaa
    23641 atattgtcga tacgcctcgg gaaggtcagg tttgacataa attccgctat caatatcgcc
    23701 aatcaccgga tcgaccatga tcaataggtc aggatggtct ttgcgtagcg cagtcagcca
    23761 ctcggcaagg attttgattt gcgatgccgt tcccatatag cccgtggtta cagcgcgcag
    23821 ctggcgcagc gcatcacgct cctgaagcgc acgcaaataa ccgctaaacc attcatccgg
    23881 aatcgcacca ccgtagaaag tgtcataatg cggcgtattg ctcagcaata ccgtcggcac
    23941 ggcaaaaaca ttcagaccgt tctgtttgat agcaggcacg gcaatgctgt tgcccacgct
    24001 gccgtaaacc acctgcgact gcacggcgac gatatccgcc tgcagcgccc tgctcttatc
    24061 gttaaacaac aacaaactac tcatttaatt ttttctcctt gccgatgatc ctcatcgtat
    24121 tccaaccgaa actttacctg attctggcag tcaaatcggc tatcacaaaa caaggataag
    24181 gtaattcaat gaagaaaatc atttgtctgg tcattacact actaatgaca ctccccgctt
    24241 acgcgaagtt aactgcccat gaagaagccc gcatcaacgc catgctggag ggattagcac
    24301 agaaaaagga tttgatattt gtgcgcaacg gtgatgaaca tacctgcgat gaagcggttt
    24361 ctcatctgcg tctgaagctg ggcaataccc gtaaccgcat tgacactgcc gagcagttta
    24421 ttgataaggt tgcttcgtcg tcatcgatta ccgggaagcc gtatattgtg aagatccccg
    24481 gtaagagcga tgagaacgca cagccttttt tacatgcgct aattgcgcag acggataaaa
    24541 cggtgcctgc gcaataatct taaatccccg ccccctggct aaaatgctct tccccaaaca
    24601 ccccggtgga aaggtagcga tcgccacgat cgcagatgat cgctaccacc accgcaccag
    24661 ggttagcttt tgccacccgc agtgctccgg caaccgcgcc gccggagctg acgccacaga
    24721 atattccttc ccgcactgcc agttcgcgca tggtgttttc tgcatcgcgc tgatgaatat
    24781 ccagcacctc atccaccaga gaagcgttga aaatccccgg cagatattcc gcaggccagc
    24841 ggcgaatgcc gggaatactg ctgccttctt ccggttgcag gccaacaatg gtcaccggtt
    24901 tggattgttc gcgcataaag cgtgaaacgc cggtgatagt gccggtcgtc cccatactgg
    24961 agacaaaatg agtgatgcgc ccgccggttt gctgccagat ttccggcccg gtggtggtgt
    25021 aatgcgcata agggttatcg ggattattga actgatcgag cagctttcct tcgccacgat
    25081 tcgccatctc cagcgccaga tcgcgcgcac cttccatgcc ctgctctttg gtgacaagaa
    25141 tcagttccgc accataagca cgcatcgccg cacggcgttc ctggctcatg ttgtcgggca
    25201 tcagcaattt catgcgatag cctttaagcg cggcaatcat tgccagcgca atgccggtgt
    25261 taccactggt ggcttcgatt aagacatcac ccggtttaat ttccccgcgc ttttccgcct
    25321 cgacgatcat cgaaagtgcc gcacgatctt tcaccgaacc tgccgggtta ttgccttcca
    25381 gttttaacca cacttcactg ccgttatccg gccccattcg ctgcaacttc accagaggcg
    25441 tattgcctat tgtttgttct aatgtactca cgatctctat ccatacgtgg tgttgcctga
    25501 tgcgacgctt gcgcgtctta tcaggtctac aggttacaaa ccttgccatt aaaaagcccg
    25561 gatcgcggga agcgcctccg ggcgtttaac attcactcaa cctatcaggc gctttgtgcg
    25621 agagcaagtt cctcatcgcg ggtttcgata cgctcgtcgc cgttatacag ccgcgcatgt
    25681 tgcagaccaa cgaataaacg ctcgccacgc tgcggggcat cgtcgccatg catcacgacc
    25741 gtcagcggtt cgttgtacca ccccagcggc tgcaccacta attgggtgta gtgacctttc
    25801 gggctggctt ccagtacctg taccggcagc ggcgaatcga gactggtacg gcggctgata
    25861 tccacttccc acgggcgcag gaagagatcc accggcccct gataagcagg tgtgtagccc
    25921 agcggccagc gatgtgcgcc aacatggaac tgcccgccgc gaatggttcc ctgcaggcgg
    25981 ttcacttcgc ccataaattc gagtacaaaa cgggtcgctg gttcgcgcca tacctgattc
    26041 ggcgcatcag cctgttcaat attcccctga ctcatcacca ctacacgatc ggctacttcg
    26101 gtcgcctctt cctggtcgtg ggtcacaaac acgctggtga atttcagctc ttcatgcagt
    26161 tgacgcagcc agcgacgcag ctctttacgc acctgcgcat ccagcgcgcc aaacggttca
    26221 tcaagcagca gaatttgcgg ttctacagcc agcgcgcgcg ccagcgccac acgctgtttc
    26281 tggccgccgg aaagctgcgc cggataacga tccgccagat gcgcaagctg gaccatttcc
    26341 agcaattttg tcactttcgc tttgatggct gcggcattcg ggcgctcgcg acgcggcagc
    26401 accgtcaggc caaaagcgat attgtcgaat accgtcatat ggcggaacag cgcgtaatgc
    26461 tggaacacga aaccgacctt gcgatcgcgc gcgtgcaggc ggctgacgtc ggtgccgtgg
    26521 aagcgaatat gcccgctggt ttgatgctcc agcccggcga taatgcgcag cagcgtggtt
    26581 ttcccggaac cggacggccc cagcaacgcg accatctgac ctgaaggaat atccagtgag
    26641 atatcgttca gcacctgggt gcgaccaaac gacttcttaa tattggcaat ctcaatgctc
    26701 atgatgttcc tcctgctgtg cgcgtttttc ctgattctcc aggcgccact gcaacatact
    26761 ttttaaaaac agggtgataa tcgccatcag cgttaacagt gccgcagcgg taaaggagcc
    26821 gacggtgttg tagtcctgct ccagcaattc aatctgtaac ggcagcgaaa gtgtttcgcc
    26881 gcgaatcgag ccggaaacca ccgacaccgc gccaaactcg ccaatcgcgc gggcgttggt
    26941 cagcactacg ccataaagca gcgcccagcg gatgttcggc aatgtgacac gacggaacat
    27001 ctgccagcca gacgcgccaa gcaaaatcgc cgcctcgtct tcctgactgc cctggcttaa
    27061 catcaccggc accagttcgc gcaccacaaa cggacacgtc acgaagatgg tgaccagcac
    27121 cattcccggc caggagaaca taatttgcag attatgttca tccagccaac cgcccagcgg
    27181 gccgttagag ccgtagaaca gcagatacac cagaccggca accaccggcg atacggcaaa
    27241 cggaatgtcc agtagcgtca gcagtaactg gcgtccaggg aagttaaagc gcgtcaccag
    27301 ccaggccagc agaatgccga acaccaggtt taccggtacg gcaatcagcg cgatcatcac
    27361 cgtcagccag atggcgtgca gcatatccgg atcggccaga ttctgtaaaa caggcatcag
    27421 ccctttgctg aacgcctgta cgaagatata aatcatcggc accagcagga tgaacgccga
    27481 taccagcatc ccgatgccaa tcagaaacca tttgccccag ttaatcgggc gcgcgtcata
    27541 acgcttcaat tgtgtcactt ccgccattaa tgacctacca cacgccgacc aaagcgactt
    27601 tgcagagtgt taattgagaa cagcagtatc agggatgccg cgaggatcac cgaagcaatc
    27661 gcgctcgccg ccgggtaatc aaactcctgc aagcgcacaa aaatcatcag cgacgtcact
    27721 tccgtcttcc acgcgatatt tccggcgata aaaatcaccg cgccaaattc accaagacta
    27781 cgggtaaacg acagcgccac gcccgccacc agcgccggag aaagctccgg cagcaccact
    27841 ttgcagaaac tctgccagcg cgttgcacca agcgtttccg ccgcttcttc atattccggg
    27901 cctaactctt ccagcaccgg ctgcacggta cgcaccacaa acggaatgct ggtaaaggcc
    27961 atcgccaccg caatccccag ccatgtatag gtgactttga tatcaaactt cgccagccat
    28021 tcaccgtaaa aaccgtttac ggaaaagagc gaggccagcg ttaaaccggc gacagccgtt
    28081 ggcagcgcaa agggtaaatc catcagcgca tcaagcagcg tgcggcctgg gaagcgatag
    28141 cgggttagga tccacgccat cagcagaccg aaaacgccgt taaaaatcga tgccacaaac
    28201 gccgacagca gcgttacttt gtaggccgcg accacctgcg ggttggtgat cacctcccag
    28261 tactgcgccc agctcatctg ggccagttgc atcaccagcg cggagagcgg cagcagcaaa
    28321 atcaggcaca caaacagcag actggtgccg aggcttaagg taaagcccgg cagcacgcgt
    28381 ctggaggaga cagcaaacat cagttacgcc ccgccgctaa cagcttgtct aactcgccgc
    28441 cgctggtgaa gtgggttttc atcacttccg gccaggagcc aaatttgtct tccacgcgga
    28501 acagctcggt ctgcgggaat ttgtctttca gtttgtccat cacctccggg ttattcacgc
    28561 ggtagtaata gtcggtgatg atggtttgcg cctgcgggct atagagccag ttcagatagg
    28621 ctttggcggc tttttccgta ccgttggcct gcacgttttt atcaacccac gccaccggga
    28681 attccgccag aatgttggtt ttcggaatca ccacttcaaa gccctgcgct tcatactgtt
    28741 tacggatgtt gttcacttcc gattcgaagc taatcagcac atcgcccagg ccacgctcgg
    28801 caaaagtggt ggttgcgcca cgaccgccgg tatcgaacac ttcaacgttt ttcaggaact
    28861 gggtcataaa ctgttcggtt ttggctttat cgccaccgtc agctttatcc gctgcgcccc
    28921 atgccgccag ataggtataa cgcgcgttac ccgacgtttt cgggttcggg aaaatcagct
    28981 tcacgtcgga gcgcaccagg tcgttccaat cgtggatatt cttcgggtta cccttacgca
    29041 ccaggaagcc catggtggag tagaacggcg agctattatt cggcaggcgc gactgccagt
    29101 cggccgggat cagcttgcct ttatcgtgca ggatttgtac gtcggtcacc tggttataag
    29161 tgacaacgtc ggcttttaag ccctgtaaaa tcgccagcgc ctgttttgat gacccggcat
    29221 gagattgttt tatcgtcagt ttgtcgccgc cgttatcttt tgcccattgt tgctcaaacg
    29281 gcggattcag ggcggcaaac agctcgcggg agacgtcata agaactgttc agcagttccg
    29341 ttgcctgtac atggcccgcc agcagcagag aagcgaccag cgcgagtgag ttctttttca
    29401 gtaagttaac ggccattgcg cacccttata aatttaatga ctttctaata gccatcatat
    29461 ttataacgga cgtgaaagga gtaacggttt tatataccgt ttggtgattt ggaagttgaa
    29521 aagggaataa gaaccgggtt taaaacgaaa tcctccccac aaatgcaggg aggaaacaga
    29581 ggtgaatcag ataccgacgc taaccgtctc cggcagtgtg ctgccgccat caatcacatt
    29641 ctgtgtaccg gttaaatagc tggattcatc cgatgcgagg aaggccgcca gttcgccgac
    29701 ttccagcgga tcggcgaggc gacgcatcgg gattgctttc gccatttcag tcagcaccga
    29761 ctctggatct tccgggttcg actggcgggc aatgctttcc gccattggtg tgcgcacgta
    29821 tcccgggcaa atggcgttaa cgcgaatacc agactgcgcg tactccaccg ccagcgattt
    29881 tgtcaggcca acaatcgccg ctttcgttaa ggcgtacgcc gtttcgccag gatcggccac
    29941 catatcgcca gtgaccgaag acatcatcac aatgcgacca tctttgcggg caatcatctc
    30001 cggcagcacc gccttcgtga cgttccatac gcctttaata ttgatgtcaa tatggaaatc
    30061 gcgatcgtca tcgctcatat cgaggaaact gcccagacga caaacgcctg cgttattcac
    30121 caggatatca atgcgccctt ctttttcctt cgcgcgtttg atagctgcgg ctaccgacgc
    30181 cgggtcacgc acatcggcga caaccgccgt acagcgatga ccacgaccac acagttcgtc
    30241 cgccagcttt tcgatctcag gggagatatc cagcaagatt aggtttgcgc catgacgcgc
    30301 aaaagttctg gcaattcctt cgccaattcc ctgcaatgcg cccgtaatca gtgctgtctt
    30361 gcccgtgagt ttacccattt taatgctctc cttgttaata cagagcgtta cactccattc
    30421 cctctgaaaa tacaggataa acaaaatatt gctgcggatc agctcactaa ttcaggaaaa
    30481 tctcaatcca gtcgctgctt tatgcatcat ttcaggcgtt gtgttagctc actgctgcgc
    30541 tgaatcattt tcaatgactc cacatcattg agttgcacaa gaccaacaaa cagtaaatcg
    30601 gtaacagaat tttgcgccgt gcgggtagac atcgacgagc tacgccattc ggtttcaccg
    30661 gaaacggtat cgagcgtgaa atgcgccagc cgccgcagcg gagaatcagc cagagaagta
    30721 atggcaatca ccgttgcccc ctgctttcgt gccgcttctg cgcataagac aatctctttt
    30781 ttactgccgc tgtaagagat agcgatttgt acatcgcctt ttttcaacgc ctgggaaacg
    30841 gtggcttgta cgtgcgtgtc ggcttcgcag gccacgcgat agccaatttt catcagtttg
    30901 aacgacagat cgcgccccac cagcgccgat ccgcccaggc ccgttatctg gataaacggt
    30961 gctttactga tgacctcaat gattttttgc agccgcgcgt agtcgaacaa cgcacaggtt
    31021 tgctccagcg ccagctcttt ttcgcgattc aatttgcggg caatcacttc cagcgaatca
    31081 tcactggtaa tcgaactgtg caggtgcagc gccgtggcat tcgttttctc ccggctggca
    31141 ctgtattcgc ctattaacgc catacgcaat tcagtaaatc cttgcgcgcc gagtttttgg
    31201 gcaaatttca ctatgctcga ctggctaatg ccgagctgtt tcgccatctg ccgtgaagaa
    31261 acggattgca gctcactcac gttggcctgc agaaagtcag cgattttttg ctcgttttcg
    31321 gtgaattcac tccccgcatt gcttattttc gtcaggtaca acatcttcca ctctcctggg
    31381 ctggcagcat aaattccgct taagttctta tgacgctctt cacactctgc gagtgtagac
    31441 tcaatgattc ctttaagtct gtcaattcgg gatatatgat tccatacatc tgcaattatt
    31501 tgatcgtaaa tagtaaggtc accaccgatg caactagaaa agatgattac tgaaggctcg
    31561 aacaccgcct cggctgaaat tgaccgcgta tcgacgctgg aaatgtgccg gattatcaac
    31621 gatgaagata aaaccgtacc gcttgccgtt gagcgcgtcc tgccggatat cgccgcggcg
    31681 atcgatgtta tccacgccca ggtcagcggc ggcggtcgtc tgatttatct cggtgcggga
    31741 acatccggtc gtctggggat tctggatgcc agcgaatgtc cgcccaccta cggcgtgaaa
    31801 ccgggtctgg tggttggttt gattgctgga ggcgaatatg ccattcagca cgcggtggaa
    31861 ggcgctgaag atagccggga aggtggtgtt aacgatctga aaaatattaa tttaacggca
    31921 caggatgtgg tggttggcat tgctgccagc ggtcgcacgc cgtatgtgat tgccggactg
    31981 gaatacgcac gccagctcgg ctgccgcaca gtgggaattt cctgtaatcc ggggagtgcc
    32041 gtttccacca ccgccgagtt tgccattaca ccgattgtag gtgccgaagt ggtcactggt
    32101 tcttcgcgaa tgaaagcagg cacagcgcag aaactggtgc tcaatatgct ttccaccggg
    32161 ctgatgatta aatctggcaa agtgttcggc aacctgatgg tcgatgtggt cgccaccaac
    32221 gaaaaactgc atgtgcgcca ggtcaatatc gttaaaaacg ccaccggatg taatgccgaa
    32281 caagcggaag cggcgttaat tgcctgcgag cgcaactgta aaacggccat tgtgatggtg
    32341 ctgaaaaatc tcgatgccgc agaagctaaa aaacgcctgg atcaacacgg cggctttatt
    32401 cgtcaggttt tagacaagga ataacccatg gccaaagaga tcagcagtga acttctgaac
    32461 accattctta cccgtgtcgg cggaccggga aatatcgcca gttgtggtaa ctgtatgacg
    32521 cgcctgcgtc tgggtgtaca tgacagttca ctggttgatc ccaatatcaa aacgctggaa
    32581 ggagtgaagg gcgtcatttt gaccagcgac caggtgcagg tcgtttttgg gcctggtaaa
    32641 gcgcatcgtg ccgcaaaagc gatgagcgag ctgctgggcg aggcaccggt acaggatgcc
    32701 gcagaaatcg ccgcccagaa caaacgtcag ttaaaagcca aacaaacctc cggcgtgcaa
    32761 caatttctcg ccaaattcgc caccatcttc acgccgctga tccccggttt tattgccgcc
    32821 ggtctgctgc tggggatagc gacgttaatt gccacggtga tgcacgttcc ggcagatgct
    32881 cagggcacgc tacccgacgc gctgaatttt atgaaggtgt tcagcaaagg tttgttcact
    32941 ttcctggtga ttctggtggg ctataacgcc gcccaggcat ttggcggcac gggcgtcaat
    33001 ggcgcaatta ttgccgcact ctttttgctc ggttataacc ccgctgcaac caccggttac
    33061 tacgccggtt ttcacgattt ctttggtctg cccatcgatc cgcgcggcaa tattatcggc
    33121 gtgctgattg ccgcgtgggc ctgcgcccgc attgaaggca tggtgcgccg ctttatgccg
    33181 gacgatctcg acatgctgct gacctcgtta atcaccctgc tgatcaccgc tacgcttgcg
    33241 tacctgatca ttatgccgct gggtggctgg ttattcgaag gtatgtcatg gctgtttatg
    33301 cacctgaaca gtaatccgtt cggttgtgcg gttttagccg ggctgttcct gatcgccgtg
    33361 gtatttggcg tgcatcaggg ctttattcct gtttacctcg cgttaatgga cagccaggga
    33421 ttcaacagct tatttcccat cctttcaatg gcaggcgcgg gccaggtggg cgcggcgctg
    33481 gcactctact ggcgggcgca accgcacagt gcattacgca gtcaggtacg cggggcgatt
    33541 attcccggtc tgctgggcgt tggtgaaccg ttgatttacg gcgtcaccct gccccgcatg
    33601 aaaccgttta ttaccgcctg tttaggcggc gcagcgggcg gtttgtttat cggcctgata
    33661 gcctggtggg gtctgccgat gggcttaaac agcgcctttg ggccgtctgg tctggtggcg
    33721 ctgccgctga tgacttccgc acaaggcatc cttccggcaa tggcggttta tgctggcggg
    33781 attctggtgg catgggtttg cgggtttatt ttcaccacgc tctttggctg ccgtaacgtc
    33841 aatctggact gatatcatga aacggacaat gctctatctt tctctgctgg ctgttagttg
    33901 tagcgtcagc gccgcaaaat accctgttct gacagaaagt tcgccagaga aagcagggtt
    33961 caacgtcgaa cggcttaacc agatggatcg ctggattagc cagcaagtgg acgccggtta
    34021 tcccggcgta aacctgctga tcattaaaga taatcagatt gtgtatcgca aagcctgggg
    34081 agcggcgaaa aagtacgatg gcagcgtgtt gatggaacag cctgtcaaag ccaccaccgg
    34141 gacgctgtat gacctggcct caaacaccaa aatgtacgcc acgaacttcg ccctgcaaaa
    34201 gctgatgagc gaaggcaaac tgcatcctga cgatcggatt gcgaaatata ttccggggtt
    34261 tgccgatagt ccaaatgaca ccattaaagg caaaaacacc ctgcggattt ctgacctgct
    34321 gcatcacagc ggcggtttcc cggcagatcc gcaatacccg aataaagcgg tcgcgggcgc
    34381 gttatattcc caggataaag gccagacgct ggagatgatc aagcgcacgc cgctagagta
    34441 tcagcccggc agcaagcata tttacagtga tgtcgattat atgctgcttg gatttatcgt
    34501 cgagtcggtc accggtcagc cgcttgaccg ctatgttgaa gagtcgattt atcgcccgct
    34561 cggcctgaca catacggtat ttaacccgct gctgaaaggc tttaaaccgc aacaaattgc
    34621 cgccacggaa ttgaacggca atacccgcga tggcgtgatc catttcccga atatccgcac
    34681 ctccactctc tggggtcagg tgcacgatga aaaagccttt tattcgatgg gcggcgtttc
    34741 cgggcacgca ggtttgtttt ccaataccgg cgatattgcg gtgttaatgc aaacgatgct
    34801 gaacggcggg ggctatggcg atgtgcagct gttcagtgcg gaaacggtga agatgttcac
    34861 caccagctct aaggaagatg ccacttttgg cctcggctgg cgcgtgaatg gtaatgccac
    34921 catgacgccg acgtttggca cgctggcaag cccgcaaacc tacggtcaca ctggctggac
    34981 cggaacggtg accgttatcg atccggtgaa tcatatggcg attgtgatgt taagcaacaa
    35041 gccacattcg ccggttgccg atccgcaaaa gaatcccaat atgttcgaaa gcggtcagct
    35101 gccgattgca acgtatggtt gggtagttga tcaggtgtat gcggcgttaa agcaaaagta
    35161 ataaaaaggc cagtcagtaa tgttactgcc tggcctttaa tcaatgaatc agaaacgctt
    35221 acagcgccat caacttgtcc agcgacggtg cgaaataata gccgccggtt accggtttgg
    35281 tgaaacgcaa catcgcatca cgcttaccat cggtatcgcc aaacatgctc agcagttgct
    35341 gctcaatgtt atgcagacgc gcgcagtagg cgcagaagta cagaccgtga gtgccactgg
    35401 cagtgccgta cggcaggctc tggcgaacaa tcttcagccc tttgccatct tctttcagat
    35461 caacgcgggt gaggtgagag gtttccggac gttcgtcgcc gtcgatctct tcgttggcct
    35521 ctttggtgcg cccgatcatc atctcctgat cgtgaacgct catccggttg agctgcttca
    35581 ggttgtgttc ccaacgctgg acaaacacat agctgccgcc cgcatccacg ccgtctttga
    35641 taaccgccac ttcgcgacgc gtctcttcac ccgccgggtt ttccgtaccg tcaacaaagc
    35701 cgctcaggtc acgctcttca acccaacgga agccgtggat ctcttctttc acttcaatgc
    35761 agtcaccaaa ggcttccatc gccgcctggg cgacagagaa gtttacgtcg tgacgcagag
    35821 aaagaatgtg gatcaacaca tcgaactggg tggtcggcgc aaggccttta ccgtagcccg
    35881 gaaaatcttt cagctcttct gccccaacgc cgccgctcag agcgcgccag gtgttgttac
    35941 caaaggcaac caccgcacca agatgcgcgt ccgggaattt cgcttcaaaa gttgccagtt
    36001 tgtcggcaaa tgttttactg gccgcacgca gggcgtcaac ttcccctttc acgttggctt
    36061 cgatccaaat cgccgcgcgg caatgttctg gcaaaatgcc actctgaacc tgagacattg
    36121 ttcctcctga aaataataat gccacgcaat ccgtggcatt gttgagcgct attttacgtg
    36181 ttttttcgcg gcggcggttt gttcagacgc aaattaacgc cgccagataa ttttactgac
    36241 tttccagttt ttcagggtat cgtccgaagg cattaaccct tcaggaccac tccattcccc
    36301 gctgaactgg tagctaatat gttgactgcc ttcggcttta cattcgacgg cacgattatc
    36361 atcaccatca gctttttggc aattgccaaa tgctttgctg taaaggtcgc taaacggtgt
    36421 accgatttta acaccggtgt cagcaggaat atcgctatcc agcacgtcaa tgcgactgat
    36481 cgtgccttga tcgccgttaa tcaccatcgc cacgttgtcg cccttcatca cttcaaaaaa
    36541 gcgcaccacg ttgccgttcg cggttttcat tccgctgcgc aggcgataat cgccatcaag
    36601 cgcatcggca atggcttgtt cttgcagtgg tgtggacgcc gttaattcac caacgccctg
    36661 ctcgctcact ttggtggacg acccaaacca gttccacggg ttagcggcag accagttaac
    36721 tgacgacatc gtggaacagc cggtcagcat caatggcata gcgcataaca ttaaacgcag
    36781 cgatttcatg tcacttcctt tggttattca ataacgttgc ttggagtgca aattcaccaa
    36841 aaagtgccgt tattctttta cttcattaaa acaagcgcgt aaacgtcgat tggtcagcag
    36901 ccagattaac gccacaatat ccgccactac cagcgccaga ccgataccgc taacggattc
    36961 accgttcagc cacagccacg gttgccagca aagtaaaacc acctgcgcca acagcaacag
    37021 aaaatagagt acacgccagg tgcgaggaaa cgtagcccgc cgaccgctca acaaaaacgc
    37081 cagcaccgcc ggaatgccag gaatcagccc cagccagaaa ttatcgtgat cgggataaaa
    37141 cagatttagc agcgcagtac cctgctcacg cgacgcaccg gcaatgacaa acagcaccca
    37201 ggttcgcgcc tgaagcaata gcacaagcca gaagagcaag ggtaaacgca ggcgtccgtg
    37261 cgcatcataa tggacaggat ggaactcagt actcttcatc ttcaatcaaa cgcttaccca
    37321 gactcagcac gtcggcgtgt tcatatccca ggcgttcata cattccgagc accatgtcgt
    37381 tatcttccgg cacattgatc tgaattttcg ggcagccacg agcaatcagc tttttctcca
    37441 gccgattaag caacgcattg gcaatcccac gcccacgaaa ctctggatgc acgccaagat
    37501 aataagcaga cccgcgatgc ccgtcataac cgcccatcac cgttccgacc acttcaccgt
    37561 ttacctcagc gaccaaaaac aaactgacgt catggttcat cttacgctcg atgtccattt
    37621 ccggatcgtt ccacggacgc agcaagtcgc aacgctccca aagggtgatg acctcttcga
    37681 aatcttcctg gcgaaatacg cgtatctcca tggtattcgt tacctttttg cgggttaaaa
    37741 ggctgattat ggcgtgaacg gtcgaattag ccaatatctg acgaaaatcg gttgaaaaag
    37801 tggcataatg gggagttgtc aactattgaa atgaaaagta aaacaattct caacatcaaa
    37861 ccgtcgtaac ggattacgcg atacgatata acatctggaa ctttattatt acaactcagg
    37921 ccgtatgagc acttttaaac cactaaaaac actcacttcg cgccgccagg tgctgaaagc
    37981 cggtttggct gccctgacgt tgtcaggaat gtcgcaagcc atcgccaaag acgaaccttt
    38041 aaaaaccagc aacggacaca gcaagccgaa agccaaaaaa tctggcggca aacgtgtcgt
    38101 tgttctcgat ccaggccacg gcggaattga taccggagcg atcggacgca acggttcgaa
    38161 agaaaaacat gtggtgctgg cgattgctaa aaacgtccgt tccattttgc gtaatcatgg
    38221 gattgatgcg cgtttaacgc gttctggcga tacgtttatc ccactttacg atcgcgttga
    38281 aatcgcccat aaacatggcg cagatctgtt tatgtcaatt catgccgatg gctttaccaa
    38341 cccgaaagct gccggtgctt cggtatttgc cctctctaac cgtggggcaa gtagcgcaat
    38401 ggcgaaatac ctgtctgaac gcgaaaaccg cgccgatgaa gttgccggta aaaaggcgac
    38461 tgacaaggat cacctattgc agcaagtgct gtttgatctg gtgcaaaccg atactattaa
    38521 aaacagcctg acgctcggct cgcatattct gaaaaagatt aagccggtgc ataaactgca
    38581 cagccgcaac accgaacaag ccgcgtttgt ggtgttgaaa tcgccgtcgg ttccttcggt
    38641 gctggtggaa acctcgttta tcaccaaccc ggaagaagag cggctgttag gcacggcggc
    38701 gtttcgtcag aaaatcgcca cagcgattgc tgaaggcgtg atcagttatt tccactggtt
    38761 cgacaaccag aaagcacatt cgaaaaagcg ataagttatg aaacccgacg cacaccaggt
    38821 taaacagttt ctgctcaacc ttcaggatac gatttgtcag cagctgaccg ccgtcgatgg
    38881 cgcagaattt gtcgaagata gttggcagcg cgaagctggc ggcggcgggc gtagtcgggt
    38941 gttgcgtaat ggtggtgttt tcgaacaggc aggcgtcaac ttttctcacg tccacggcga
    39001 ggcgatgcca gcttccgcca ccgctcatcg cccggaactt gccgggcgca gtttcgaggc
    39061 gatgggcgtt tcactggtag tgcatccgca taacccgtat gttcccacca gccacgcgaa
    39121 tgtgcgtttt tttattgccg aaaaaccggg tgcagagccc gtctggtggt ttggcggcgg
    39181 cttcgattta acccctttct atggttttga agaagacgcc attcactggc accgcaccgc
    39241 ccgtgacctg tgcctgccat ttggtgaaga cgtttatccc cgttacaaaa agtggtgcga
    39301 cgattacttc tacctcaaac atcgcaacga acagcgcggt attggcgggc tgttctttga
    39361 tgatctgaac acgccagatt tcgaccactg ttttgccttt atgcaggcgg taggcaaagg
    39421 ctacaccgac gcttatttac caattgtaga gcgacgtaaa gcgatggcct acggcgagcg
    39481 cgagcgcaat tttcagctct accgtcgcgg tcgttatgtc gagttcaatc tggtctggga
    39541 tcgcggcact ctgtttggcc tgcaaactgg cgggcgcacc gagtcgatcc tgatgtcaat
    39601 gccgccactg gtacgctggg agtatgatta tcagccaaaa gatggcagcc cagaagcggc
    39661 gttaagtgag tttattaagg tcagggattg ggtgtaactc cctcaccccc attcccgcat
    39721 ccgctgatgc aacgtcaacg acggcttctc ggcaaacagc tgctggtaat ccgtggcaaa
    39781 ttgccccaga tgccagaatc cccactgcat ggcggcgtct tttaccgttg tgctttgcga
    39841 ccacgggctt atcagttcgc ggcgtacggc gttcaggcga atgcgtttca gccacgcgtt
    39901 tgggccaatg cctaaaatag cgtgaaacgc gttttgtagc gtgcggcgac tgacatgcag
    39961 ttgattacac aagtccagca ccgtcaccgg ctcagacata ttttccagca catattcacg
    40021 ggcgcgggaa agtaatctac ggtaactctg atgactgatg ctttccgccg tcaccattgg
    40081 ttgcgcgtct tccagcatcg cccccatcgc cattagcaaa ttatccccca gcacttttcg
    40141 cactgcaggc tgatggagat tttccgggtt ctcgcaaaac gtcgccagcg cctgttggac
    40201 aaagccccac agcgcggctt tatgctgctc tttcacttcc agcgccgact ggctacgcag
    40261 catatgtaat acccgatccg ggttatgcaa aaagttagcc tgccgggtga tgacatcttc
    40321 agaaagcacc acgcccagga tcgtgtaatc atccggcgtg ctcagttcaa attcagtgcc
    40381 accagggcgg gtggcgattt ccgcgcttcc cagacattgc gaaccgataa atccctgctc
    40441 accgcgcgtc gccggaatgc caaaccagaa cgagttcggc cagaccaggc acgactgacg
    40501 cagcgccaga ccggtgtatt cacgaaaaac ctgaatatca tcgagtagaa tttccgtaaa
    40561 ttcaccatga aacttgcccg gatgcagctg atcgtaaatc tgctgccagg cggtaatcgt
    40621 taaagcatgt tcatagacat ccgttgtctg tcgttgatga acattatcca cttcgacctt
    40681 cggcgtgagc ttcaggtttt cgggtaaggg ttcatgataa agatggtgca aattggctgt
    40741 acgggtcttt ttcatgatgt taatgccggg tgttgtagga cacccggcac ctccgacagg
    40801 ttaatggggc ttgagacgat aacgactact gcgtttacgt aacgtcccgg cagaaaagag
    40861 ctgttcgagc gcatttctgg ctttttccag cggccagcca aagtgggcag ccacttctcc
    40921 tgccgtcatt ccctgacgta ctgatgttaa cagcgccaac aactcgtcag cagattccgc
    40981 gacgataact ggcgcgtcgg gttcccttac gggttgcttc ggctggcggt taaagccagt
    41041 aaccagccac tgggtgtcat cgtctggccg accgatctcc ttgcggctga tgacacgccc
    41101 ggtacgcatc gcggcagcac aacctgcgtc cagcgccgca cgacacgccg ccagatcgcc
    41161 ttccaccacc agcgttaagc gaccagggtc aagcacttcg tgactgagca gacgaacgtt
    41221 agctgctttg agcatggcat ccgctgcatc tatcgcggcg accatgccgt ccacttccag
    41281 caatcccagt gcattgatca tcggcaacct ccgttacgca cgctggattg gattacgcgc
    41341 gatttccagc actgcatcgg taaaggcgtt acaggctgct ttacacgcgg cctggctacc
    41401 ggttaaaaat gctgccgagt agttggtttc agacggtggc gggacatagg tcaccagctg
    41461 tacgtcggca gatttcaacg ctgcatcaat gccataggtc gcttccagcg gcggtgccac
    41521 cagatacgcc atcggatcgc caagcgtgat cccggcggtt gatgagagat aagaaccggt
    41581 acgcgaaacc acatgcgcca ggaaggcggt gttttctgcg tcattggccc actggaatgc
    41641 tgcaccattt tcaatatgtg caaccatcgc atccagaccg gcacgcactt ccgccgggtt
    41701 tggcccacca agcataatca gcacttcacc ggcagtcggt gacgggccgt gtgcagcgcc
    41761 cgcatacagc gagcgaccat ataccacttc caccatcgcc tgcttggtcg cttcatcagc
    41821 ggcaatgtag gtgacgtcat cggaatcagc agaaatcagc ccgaggctac gaatatgcgg
    41881 cggcaatttt agttcacgcg caaattcagc gttaacggag gcaatcaccc gcatggcggt
    41941 taccgacggt cgaatcaaat ctaaagctgg catgatgcct ccttatcggg tcatgttgat
    42001 gccggatgct ttctgctcca gcatacgttt cgccaaatcc acaatgacgg ctgcggcttc
    42061 aaccggcggc gtgccgccct ggtgaatgtt agagatacag gtgcgatcgg cctcaacggt
    42121 ggtcgccata cgcggcgagt aaacggcgta gcaggagagg ctttctgact gccctaaccc
    42181 cggacgttcc cccaccagca ggatcaccac tttcgcgccg aggatctcac cgatctgatc
    42241 ttcaatcttc acgcgaccat aacgaacaaa gaatggcgtg ccgactttca gcccggcctg
    42301 tttcaggccc gccatcagcg gcggcaggat ctcttcgtag ttcacggtga tggcatcagt
    42361 tgacaggcca tcagaaataa cgacctgtac gtccggatta gcaacacact gtgctttcag
    42421 cgcttcaaca gcttctgcac acaggcggcg gcccatatcc gggcgagtca ggtagagatt
    42481 tttgtcgcta atttcagagc gtacttccag caagccctgc gctttcaccc actcttccgg
    42541 cacttctttc agtacggtat ctttcgaacg agagtgatcg gccaggaaac gcagcagcgc
    42601 ctgggtacgt ggacgcggac cggcacgacc ggtacaaacg cgggccacgg tgctgcggcg
    42661 cagttctgtt aatacgtctg cgcgatgcgg gttttcaaca ccaatccacg cttttgcttc
    42721 agcggaacct aaatccagca cacagctttc cgaggtcacc ggtgccgcac agttggtggt
    42781 ggcgcacttt gcttctgacg gcgcgggggc cgtttgtccc attgacgcca tcacgctgcg
    42841 tacaatttct tcaatctgtt tttgatccat gatgtgttat ctccgcgtca tcagaagaac
    42901 agtgacggat cgcccgcccg tttggtcagg cgaccgtttg ccataatgcc catgctttcc
    42961 agccagcgtt caaactccgg tgacggacgc aggttgagta actgacgcac agtggcggta
    43021 tcgtggaatg cggtggtctg gtagttgagc atgatgtcat cacccagcgg catccccatg
    43081 atgtagttgc agcctgcggt ggcgagcagg atcatcaggt tttcgttgag gttctggtca
    43141 gcgtcagcgt ggttggtgta acagcagtca cagcccatag agatgccgct cagcttgccc
    43201 ataaagtgat cttctaagcc agcacggata atctggcggt cgttgtagag atactccggc
    43261 ccaataaagc cgaccacggt gttgacgata aacggatcgt aatgacgcgc cagcccatag
    43321 ttacgtgctt ccatcgtcac ctggtcagcg ccgaagttag cgccagcgga tagcgcagag
    43381 ccttgtccgg tttcgaagta gaggcagttt tccccggcga tacggttgaa ctctgcaccc
    43441 actgcgcgcg cttcgtcgag catcgccagt tccacgccaa actctttcag ccctttttcg
    43501 ctgccacaga tactctggaa aatcagtccg ccaggcgcgc cgcgacgaat cgcttcgatc
    43561 tgggtggtga cgtgcgccag tacgcagccc tgagttggga tgttgaattt gtcgatcacg
    43621 ccgtaaatgg tgtccaacac gcggcttaag ttttccacgt cgtcagtcac cgggttaacg
    43681 ccgatcaccg catcgcccac cccgaaggaa agcccttcgt agatttgcgc ggcgatactt
    43741 tgcacgtcgt cacgggtgtc gtttggctgc aagcgggcgc tgaaggtgcc cggaataccg
    43801 atggtggtat tggccttttt gattaccggc attttcttcg cgccgtagat caggtccgcg
    43861 ttggagcaaa tcttcgctac cgccgcgacc acttccgagg tcagcccttt gcgggtaaag
    43921 gcaatgtcgt ccacgctggt ttcatcgctc agcacatact cacgcagttc gctgatgctc
    43981 cagtttttaa tctggttgta ggccgtttcg ttaacatcgt cctgaatcag ccgcgtcacg
    44041 cagtcatctt cataggcaat caccggatta ttgcggatgt ccgcgacggt catttccgac
    44101 aacacctgct ttgcagccac gcgctcctgt gaacttgccg ctgcaacgcc tgccagcaca
    44161 tcccccgaac gcagttcgtt ggctttagcc agcacctctt ttacatcctt aaactgatat
    44221 acattgccga acaatgtggt ctttagtttc ataagtcgtt ccctcaggaa ggaaatgcga
    44281 gtgatttcac cgtcaccggc acaaccgatc cgccaaaaag aggcgtacca atgtcgatat
    44341 agtcccccgc gcggacaatc acttcgtcaa tgactgccaa cgggagttgt tgtagctgtg
    44401 ggcgcaacaa catgcccaga gctttaccaa agtcctgccc ggccaccacc agcaggggat
    44461 gcggattcgg aaaacgcgcg acgaaatcga ccagcgcgtt gatgaccgtc agtaccgcgg
    44521 cgtaacgcac aggcagcgag gcgggaagcg ccagcacgta cgcgtcagtt ttgggatcaa
    44581 gatccagctg aagcagcgcc tgttgccagg cactcaccag atccgtttca tcaatcggga
    44641 tcgccaccgg caaattgcgc agcggcagtt gtacgccctc aagccagatt gtgctgccag
    44701 agagcgaaag ggtatgtgcc cccgcgccaa tcaccgtggc acgcacggtt tgcgccggaa
    44761 actgcacgtt catctcacgc aggcgcggat ggtcatgcag cgccgttgcc agaagcgggc
    44821 caatatcggc aaaacagaac gggtcggcgg gctggtggcg ataacattcg cccacgccgc
    44881 cagaaagcgt aatgatttcg ggcgtaacac ctgcgggcag caaaccggtt tgcatcaatg
    44941 cctgcgcgag cggcgaaagc gttccgtcaa tcacttcgac aatcagctct gccatccgcc
    45001 gggtaacctg caccagctgc gcgccggtca gcgaacgggc gtcagtgcct gcaccgaagc
    45061 attcatccac aatcatctgc cccggtttat gagcgtaaac cacgcgcccc tggctgtcgg
    45121 tttccagcag gcgaccaccg acgttgaggc aggcggtgcc gctgattttt ccggcatcga
    45181 acagggcgta gttcgcggtg ccaccgccga tgtcgatatt cagtacccga cacagccgtt
    45241 gttcagaaag ggtttgcgcc ccagctccgt gaccggcgat cacggattcg aggtgcggcc
    45301 cggcgctggc aacgacaaaa tcccccagcg attgagagag cgccatcacc gccgggcgag
    45361 cattgcgggt tttcgcgctt tcaccggtga tgatgatggc accagaatca acgctttccg
    45421 gcgcaatgcc cgcagcctga tattgctcta aaatcagcga cttcagttcc gcttcttcta
    45481 aaccaccctg tttatcgaca ggggtaaaga acaccgggct ttgccagcta atttcgcgtt
    45541 tgatgaattc gtagcgcggc acctgcgaca ccgccgcacg gttaaccagt tccaggtggg
    45601 agaaaatcac ctgggtggtg gtggtgccga tatcgatacc gacgctcagt agctggcgag
    45661 tgttcacgat tgtgcctccg cttcggtttt agctgcggtc gcgtcgtctt ttggcaccag
    45721 catcatcgcc acgccaatcg ccgttacgcc gccgatcaac ttgccgacaa tcatcgggaa
    45781 gatcatggcg ttcatgttgg cagcggcgaa gcctaagtgg tcgcccaggg cgaaagcagc
    45841 ggaaacggcg aaggcgcagt tgatgacttt gccgcgggta tccatctgct tcatcatgcc
    45901 aaacatcggg atgttgttgg caagcgttgc caccatgccg gctgccgcga tgttgttcat
    45961 attcagcact ttaccgacgc tcatcagcgg tttttcaaac cagcgagtca gcagcagcac
    46021 catcggatac gcccctaaca gaacgcagga gatagaaccg ataacttcaa tggcgcgcat
    46081 cacctcaccg ggtttatcgc caggggccat aaagataggg tccagaccgg ggatcagttc
    46141 ccagccaagg aggaatttca ctaccgcagc ggcaagaccg agggtgatca atgcaacgag
    46201 gaatttggca aagatctgga agccgttgat cattttttcc gggatgaatt tcagccccag
    46261 cgccaccaga atcgcgacaa tgatcaccgg gatcatgttc atcaggatca gggcgaaagt
    46321 gaattccacc ggctgaccgt tgatctgcac accggagtac atagcaacca gaccaccagc
    46381 aatacagcca atcggaatgg tcacaatgcc cgccagcacg ccgagcgcca gataacgacg
    46441 gtcagaaggt tcgataatgc cgagcgccac cggaatggaa aacacaatcg ttggccccat
    46501 catcgacccg agaattaacc cagagtatag ccacgctgct acgtcgccgc ccgccagctc
    46561 tttggcgagg aagaagccgc ccatatcgca cgccagcagc gttccggcga acattgacgg
    46621 gtttgcgccg agcatttcgt aaaccgggat aattaccggc ccgagtacgt gagccagtac
    46681 cggtgccagc gcggtcatac cgaccatcgc caggcccagt gcgcccattg ccataaagcc
    46741 ttcttcaaac tgaccgccgg atccttcgat acttttaccg aacttaccga ggaaacgagc
    46801 agaaccgccg aactgcgaca ggatcctgtc tacggcagct atcagcataa agaacatcat
    46861 gatgtacatg atgatttcgt taattcccat cgcctttact ccctgttagt tgttatttat
    46921 tggcggatgc ggcgtaaacg ccttatccgc cctacatgtg caatcccgaa ggccggatca
    46981 gacgcggcaa gcgtcgcatc cggcatttat tgcgccgctg cgtacaagcc gacaatctgc
    47041 tccaggctgg cggtacgcgg gttgctgcgc agacaaatat cttcctgcgc ggcctgcgcc
    47101 catgcaccgt aatgcgcaga tgtcgcgcca acatcgccca gtcgtttacc aatcccaact
    47161 tccgcaatca gctcacttac cgcgttaata gcgtcatgat cgtcggattt tttagttcgc
    47221 agtgcccgac caatctgact aaagcgttca cgacaaacca tccggttaaa ttccatcact
    47281 gttggcagca acatggcgtt cgccagaccg tgcggaatat gcagcgccgc gcccggctga
    47341 tgcgccatcg cgtggcacaa cccaagaccc gcactggaaa acgccattcc cgccatacat
    47401 gaagccagca acatgctctc gcgcgcggca aggtcgtgac cgtagcccac cgctttcggc
    47461 agcgatttgc caatcatcgc aatggcacca atcgccagac tgtcggtaaa cggtgtagcg
    47521 ttcagggcgc tgtatgcttc aatggcatgg gttaacgcat caatgccggt catcgccgtg
    47581 acatgcgacg gcacaccttc ggtcaatgcg gcgtcgagga tcgccacatc cggcatcagc
    47641 gaggcatggg ctaacacctg cttgcgcccg ctcaccgcgt cgataatcac cgttacattg
    47701 gtggtttcag agccggttcc ggcggtagtt ggaatggcaa tcagcggcaa gcgcggttgc
    47761 agaacgctgg tttctgacat ctctgccagc gtgctatccg ggttcgtcac cagcaacgcc
    47821 acggctttcg ccgcatccag caccgagccg ccgccaaatg cgatcacccc gtcacaacct
    47881 gactcacgca actgcgccac ggctgcacac acgtcggtaa tgcacggttc gcccaccgga
    47941 catagccaga gcgtcatggc gataccttta acggccaggc tacgcgtcag cccggcggtc
    48001 atccctgcct gatgcaaaaa gctgtctgcc atcacgaaca gatgtttcag cccacgcgtt
    48061 tgcgcttgct gtccgcaact gctcaccgag cccggaccgc aaagcgtcac cggtggaacg
    48121 ctaaatgttt ttacccgttg cagattcagg gtatcgaacg cctgaaagag cgcggtctgc
    48181 aattcatttt gcatatagcc cctccgcttt ctctcgccca ctactggcga tcgccagcgg
    48241 ggtcataaac aaactgtgct gcggtaaatg cacttgtaac gccgggaatt gtttgcgaaa
    48301 cagttccgcc acgcccggtt gcatacagga gccgcccgcc agccataaat cagtaatgcc
    48361 ctgcccttca atatgccggg cgacaatgtc cgccattttt tcgtacaccg gtttcaccgc
    48421 aggccaaatc tcttcaccgt gaccgcgctt gtactgctcc gcctcttcca gcgaaatacg
    48481 gcgatttccg gcgagagtca gagagatgtg atgtccgccg gtggcttcat ccgccgagta
    48541 cgtcactttg ccctttttca cgatggcaat gccggtagtg ccgccgccga tatccactac
    48601 accggcgttg tccagttgca gcagatccgc aactgcggtt ggctcatcca gcacatggct
    48661 cacttccagt ccagcagact ccagcacgtt gatggaaata cgcgggtccg tacctggagg
    48721 aaatgaagtc gccgcatggc taaaacggcg accgaattgc tgctcgagcg tgtcgagatg
    48781 gcgacgaaca atggtgacag cgccgaagaa atcccagacg atgccgtcgc ggaccacatc
    48841 ggcccagtcg aggcataccg ccaccggctg accgtcgcgg tcaacaacca tcgacaccac
    48901 atcgcaggtg cccagatcca cccccagcca cagcggagat tccgtcgcgg caggcgtctg
    48961 gttacacagc gttgccgccg tttgcagacg tggggtgagc cattgttcgt cgtgcgccat
    49021 cggttactcc ttaaacaatg cgaaacgcat cgactaatac acagcgacgc agacggacaa
    49081 acgtccgcgc gctggttacc ccttcaccgg ttggcgtggt gatggtcatg gtggtccagc
    49141 cttccccgcc cagccccagc ccggcaatgc acggtccgtt cttaacgaaa atgctggtat
    49201 caattgcatt cgccatctgg ttcatgtttt cgatgttgcg cgagtgcatt gccgccgtgt
    49261 ggtggcaacc gccttccagt ttcaccgcca gcgcaatggc atccgccacg ttggcgacgc
    49321 gcacgacggg cagcaccggc atcatcagtt cggtcacggc aaacggatgt tctgcagtgg
    49381 tttccacaaa cagcaaacgc gtttcttgcg gcacgttcag gccgattgcc gccgcgattt
    49441 tcgccgcatc gcgaccaacc cagtcacggc tgacggtgcc ttttccgcgc tcgtcgatat
    49501 ttttcagcaa caccggttgc agctgctgcg cctgttctgc ggtcagtttc accgcgtgct
    49561 ggccttccat cagacgcatc agttcatcgg ctacgctatc aacaacaatc agtacctttt
    49621 cgtcggcaca aatgatgttg ttatcgaaag aagcgccttt gacgatggac tgagcggcac
    49681 gggcgaggtc ggcggtttca tccaccacta ccggcgggtt gccagcgcct gcggcaatca
    49741 gacgtttatt ggtgtgttta cgcgccgctt ctactaccgc ttcgccgccg gtcaccacca
    49801 gcaggccgat acccggaaac ttgaacaagc gttgcgcggt gtcgatatcc ggatttgcca
    49861 cagtaaccag taagttttcc ggcccacctg cggcaacaat cgcctggttg agcagcgtaa
    49921 tcgcccgctg ggagactttt ttcgccgccg gatgcggggc aaaaatgacg ctgttgcccg
    49981 cggcaatcag gctgatggcg ttattaatta cggttgccgc cgggttagtg gacggagtta
    50041 ccgaagccac cacgccccag ggcgcgtttt caattaaagt caggccgttg tcgccggtca
    50101 gcacctgcgg agagagacac tcaacgcctg gcgtgccgcg cgcctgagcg acgtttttgg
    50161 caaatttatc ttcaacgcgg cccatgccgg tttcactgac ggcaagttcc gctaaatctc
    50221 tggcgtgttt ttcgcctgcc tcacgaatgg cagcaatggc taactggcgc attgccacgc
    50281 tttttaaccc ttgctgggcg actttggctg ccgcaacggc gtcatccagg gacgcgaaaa
    50341 cgcccatctc atgaacggcg gcggacggcg tgtcactgct ttgcattttc agcagtaccg
    50401 ctttcaccac ctgttcaata tcctgttgat tcatgatgtt ctgccttatt tgtggaaaat
    50461 tacctgaccg ccagacacca cctcatcgac aatgccaatc acgcacagat cgaccggtga
    50521 cgtttcgctt ttatgcgcct ggcgggcgga actgccactc accagtaaca cccactcccc
    50581 ggttcccgcg ccaatattgt cgatggcgac ggcgcattgc ccgtcaggat taccttgtgg
    50641 atcaatcatt tccaccatca gcaatttgtc atgcgccagt ccgtgatggc gtacggtaca
    50701 aacaatttgt ccagtgacga ctgccagttt catacccgcc tccgtggcgt atttcaggta
    50761 aaagctcccc ctaccctccg cagaaggtaa aatgaaaaag gagagagcgt gacgcccgaa
    50821 tcgacgtcac acagggtgat tacaggttgc tgctatcgcc tttcaggccg atcgggaaga
    50881 cttcttccag gtcaccgtgc ggacgcggga taacgtgtac ggataccagc tcgccaatac
    50941 gctgtgccgc tgcggcaccg gcatcggtcg ccgctttaca ggctgcaaca tcaccgcgaa
    51001 ccatggcagt acacaggccg ccgccaatct gcttaacacc aaccagcttg acgcgcgcag
    51061 ctttcaccat cgcgtcagaa gcctcaatca gtgcaaccag gccccgggtt tcgatcattc
    51121 ctaatgcttc cattgtgctt tcctctttat cagggtccag aacgggaccg ttcattcaac
    51181 cagtgtttgt aaactgcttt cgcggttcac ttttgtctga cgcggcacag ctgccaccag
    51241 cgccagctcg ataatttcct gcacgctaca accacgagag agatcgtgca tcggcgcggc
    51301 aagtccttgt atcagtggcc cgacggcacg atatccgccg agtcgttgtg cgattttgta
    51361 accaatattt ccggcttcca gcgacggaaa aaccatcaca ttggccttgc cctgtagcgg
    51421 gctggcaggc gctttttgcg ccgccacttc cggcacgaag gcggcgtcaa actgtaactc
    51481 gccatcaacc accagctttg gtgcgcgctc acggacgatt tctgtcgcct gctggacgtt
    51541 ggcaacgcag gggtgacggg cgctaccgtt actggaaaac gacagcatcg ccacgcgcgg
    51601 ctcttctccg gtgatggcgc gccaggtttc ggcactggca agcgcgatat ccgccagctg
    51661 cgccgccgtc ggctgtggca ccacgctgca atcggcaaag cccaacgccg ggccgctgta
    51721 ctgtggcaac atcaggaaaa tggaagagag cgttttacag cctggctgca agccgataat
    51781 gcgtaatccg gcacgcagca catttgccgt ggaagagagg ttgcccgcga tacagacatc
    51841 cgctttaccg gcgctgacca ttgctgcggc gaacatcaga gggtcggtaa gtttttccag
    51901 cgcatccggc ggcgtttttt cgcccgcgcg ggccagccag cgatgagcaa attcttcccg
    51961 cattgcgagg ttgccatgcg gatcaatcac ctgtagcccg tccatcgcca cgccgtgact
    52021 gagcgcaaac tgacgaagtt caaacggatt ggcgaccaga atgggcgttg ccagaccttg
    52081 ttgatgtaaa tattgcgcgg ctttcagcac acgttggtct aacgcatccg gaaaaaccac
    52141 tctggcgggc gctcgcaacg ccagttcacg acaacgttca ataatcattg cgtttccccc
    52201 agtcgttgtt ggatctgctt caccgttagc ggctgcttcg ttacgctcag gatcatcatc
    52261 acgtagaccg tgctcgaaag gcggttcagc gcctgcaaaa tgtccgggcg taatacttca
    52321 aagctgcggg tgataaagac ctgcgccgct acggtttcgg tttcccgcac tttggtgcgt
    52381 agcagattga gcaacgcagc atcgcgcccg tggctggctt ccggcaccag atgatcgtgg
    52441 tcgagatagc gcagcggctg atgagaaagc cgatgcagat cttcgtcaga aagcccgaca
    52501 atcgcctggc agcccagcgg ttcccccagc gcatcggcgc gcataatgtt gccaagacgc
    52561 gaacggatat ccgccagcca cggctgccac ggttccgcca gttcaatttg cagccacact
    52621 gccagcgcaa tggtgctgtc gagaaccgcg cgaaaaccca gacgcggatc gcttttggcg
    52681 accatttttt ccgccgacag gtgagtcagc gtatccggct tttttgccac cggctggcgg
    52741 cacagttcgc agcacgcctg cggatgttca tcgctactgg tcagcccatg aacgggctgc
    52801 ggctgctgct gttcatcgtc aacaaacagg cggccctgct cgtcaataaa cttgatgcgc
    52861 agatggcggc tttccagcaa ctcccgggca gagggcgtca ggcgactgtc cgcagggaga
    52921 tggatctctg cgccttcgct gagcgtatgg ttcgctctta gccatgcttc ggtgatgaaa
    52981 tctttcatag ggattgccag ttagccggcc aggcgacata caggaatttc acgctggatg
    53041 tcgtaccaaa ttcgatgctg gagcctttcg ggataaacat cacgtcgcct gctttggcga
    53101 tcatggtttc gccttcgtgg cgaacatgta gctccccttc cagcaccatg tcgatttcgt
    53161 cgtagttcag ggtccacggg aagaatgcgt tctcccactg cataaaaccg gcggccatgc
    53221 tgctgccgtc gtctccggtc acaagatcag tcaagcccac gcaatgcggc tctgcgccgt
    53281 cgaaacgacc aaacttgacg ctgctgccat caatcacttt gatgccgcct ttgccggtca
    53341 ccgatttgaa gctcggctgc attgcgccct gctccagcga ctgtttttcc ttcatcactt
    53401 tttccatcag ctgtgcgacc aggctttcgg taaactggcc ttccggcagc tgggcaatga
    53461 tggtttcgcg gatgcgctgg ctttcggttt tgtcggctgg cacgctggcg ggcacggacg
    53521 ccgttaccgg gatcgattca tcacattcgg tgatggtaaa acccagtaga tccgccactt
    53581 cacgggcttc cggggtaata atgctggcgc gcagaaccac ggacattgcc tgttcgccgc
    53641 gcgcgtgtgc ttcacgaata tcattcgctg tgatgagttt tttcacctgc ttcctcctgt
    53701 ttggtgagag atgccagata atccaccagt tgctgcacgc tttgcgggtc gtggctgttt
    53761 agctcgaaaa tcggctcttc gaaacccgtc tcaagcaata actttcgcgt tgcggcgacg
    53821 tcagcatctg gcatgtccgt tttactgatg acggcgattt gccgtttgct gacgccaata
    53881 tccagcaacc cggcaggcaa ccgactttcc gggtcatttg cgccatggac atagatcagc
    53941 atgtctacat cctgtagcgt ggtaattaag gcgtgatacc agcggggatg gctaaaatat
    54001 tcacccggcg tatcaatatc gcccttatca ttaaattcca cggcctgtgt ttttctggcg
    54061 agggtataat tcccctgcaa cgcattaaat aacgttgttt ttcctgcccc gacgctaccc
    54121 acaaacgcaa ttcgtttcat gaatgcctcg tattaacttt tggtcatttc acaaagcgta
    54181 taatttaata agcgacctaa accgctgacg gtttgtgata acgcttcttc tactgcgccc
    54241 accgagccat aaatcaccaa tgcaccgctg aagcggtcga gaaaaccgat atgcacatca
    54301 gcggctttca gcgctaaatc accggcgatc atcgccgttt cgccgggagt tagcgtcata
    54361 atgccaattg cgcccgcatc cggaacgccg attttcttcg ccagttcctc accgggatgc
    54421 gcgatgagat gcgccagtgt gacctgtttg ccgggcacaa attcctgaat gatgcgttct
    54481 ttatccatga gtcgccgcct gtgaaaaact ttcgtgtatc gtgacaattt ttcgtcagag
    54541 taaataacaa aattcggcaa gcgcgtttaa aaggtgaggt agatcactaa gaatggagag
    54601 atattcgctg tggtgcataa actttcatat gcaacgcatg aatataaata aaataatatc
    54661 gagatattat atagactgct ttttttatta ttcgcaaact cagtaataag ctttactaat
    54721 atattgcagc caaaccgctt accctgaata ttcagggtaa gcgtgagagt taaaaaagaa
    54781 ttacagcggt tgggtttgcg cttcaaccac ggccagcgcc accatgttga cgatacgacg
    54841 caccgatgcg atcggcgtta acacgtgaac cggtttcgcc acgcccatca gcaccggacc
    54901 gacagtcaca ccttccgagc tggaaacgcg cagtaagttg taactaatgc gggcagcttc
    54961 catgttcggc atcaccagaa tattggcaga acctttcaaa gggctgtccg gcatacggtc
    55021 gttgcgaatc gcttccacca gcgctgcatc gccgtgcatt tcaccatcaa tcatcagatc
    55081 tggcgcacga tccctgacca gttccagcgc ctgacgcatt ttgctcgacg acgggcagtc
    55141 agaagaacca aagttggagt gcgacaacag agcaacgcgc ggctcaatac caaaacgacg
    55201 gacagtttct gccgccatca aggtgatctc cgccagctct tctgcgtctg gttcgtcatt
    55261 gacgtaggta tcggcaataa aggtgttacc actcggcagc agcagcgcgt tcattgcacc
    55321 tgcggtgtga acgccatcgc gataaccaaa gacatttttc accacgctaa aatgttcatg
    55381 ataatcgccc accgtaccgc agatcattgc atcggcttcc ccacgctgaa ccatgatcgc
    55441 gccgatcact gtcgggttac tgatcagcgc ccgctgcgcc tgttcctgag tgacaccgcg
    55501 acgcttcatg atctggaagt attcggtcca gtactcttta aagcgcggat cggattcgtt
    55561 attgacgatc tcaaaatcaa cgcccgcttt gatctgcaag cccagtttct gaatgcgcat
    55621 ttcgatcacg ttcggacgac cgataaggat cggtttcgcc agtcccagcg ttaccagttc
    55681 ctgagtggca tgcagaacgc gcgcctcttc cccttctggc agaacaacgc gcttcggcgc
    55741 tttgcgagcc tgggagaaaa tcggcttcat aaacaggttg gttttgtaaa cgaactcagt
    55801 cagcttgtcg atgtagacgt cgaaatcagc aatcggacgc gtcgccacgc ccgactccat
    55861 cgcggcttta gcgaccgcag gagcgatctt aacgatcaag cgcggatcaa acggttttgg
    55921 aatgatgtat tccggaccaa agctcagatc ctgatcgcca tacgctgaag ccaccacttc
    55981 gctctgttcg gcgtgggcca gttccgcaat cgcgcgtacc gccgccagtt tcatctcttc
    56041 attgatagcc gttgcgccaa catccagcgc gccacggaag ataaacggga agcaaaggac
    56101 gttgttgacc tggtttggat agtcagaacg accggtgcaa atgatggcat ccggacgcac
    56161 ttctttcgcc agcggcggca gaatttccgg ttccgggttc gccagcgcca ggatcattgg
    56221 cgcacgagcc attttcttca ccatttcctg ggtcagcact ttcgggccgg aacatccaag
    56281 gaaaatatcc gcgccttcaa tcacatcatc gagggtacgt ttgccgtcat ccaccaccgc
    56341 atatgcggct ttggtttccg ccatgtttgg ctcacggccc tgatagataa cgccttttga
    56401 atcgcaaacc acgatgttat gtttttgcag acccagcgct accagcaggt tcatacaggc
    56461 gattgctgcg gcacccgcgc cggaaaccac catccgcacg tcggagatgt ttttctccac
    56521 cacgcgcaag ccgttgagga tggcggcagt gctgatgatt gccgtgccgt gctgatcgtc
    56581 gtggaatacc ggaatattca tccgctcgcg cagtttctgt tcaatataga aacattctgg
    56641 cgctttaatg tcttcgaggt tgattccgcc gaaggtcggt tcgagcgcag cgacaacttc
    56701 aataaatttg tccgggtcga gttcatcaac ttcaatgtca aatacatcaa tcccggcgaa
    56761 tttcttaaac agaacgccct tgccttccat caccggtttg ccagccagcg cgccaatgtt
    56821 gcctaacccc agcaccgccg taccgttaga gatcaccgcc accaggttac cgcgcgcggt
    56881 atatttgtag gcttttaacg ggtctttttc gatttcaaga caaggtgcgg caacgcctgg
    56941 tgagtaggcc agcgccagat cgcgctgcgt tgccagaggc ttggttggag aaacctggat
    57001 tttccctgga actggaaatt catggaaatc aagtgcactt tgttttaact ggtcatccat
    57061 ttggttgttc ctttcacgta acgttcacaa ataaagtgtg tgggcaacag cccctgccca
    57121 caacgtggcg cacattatta ccctgccgga gtctacagac tttgagcaag tccaaactct
    57181 caccattaat ataatgtttt ggtaataatc ctataacact gatgttacct gcttaatcca
    57241 gcaataccat gcctgtctgc tatgcttttt tgatgcgttt agcgaaattt ctcagaagtg
    57301 tgaattaacg cactcatcta acactttact tttcaaggag tatttcctat gaacgagtta
    57361 gacggcatca aacagttcac cactgtcgtg gcagacagcg gcgatattga gtccattcgc
    57421 cattatcatc cccaggatgc caccaccaat ccttcgctgt tactcaaagc tgccggatta
    57481 tcacaatatg agcatttaat agacgatgct atcgcctggg gtaaaaaaaa tggcaagacc
    57541 caggaacaac aggtggtcgc agcgtgtgac aaactggcag tcaatttcgg tgctgaaatt
    57601 ctcaaaatcg tacccggtcg cgtgtcaaca gaagttgatg cacgcctctc ttttgataaa
    57661 gaaaagagta ttgagaaggc gcgccatctg gtggacttgt atcagcaaca aggcgttgag
    57721 aaatcacgca ttttgatcaa actggcttcg acctgggaag gaattcgcgc ggccgaagag
    57781 ctggaaaaag aaggtattaa ctgcaacctg acgctgctgt tttcttttgc ccaggcgcgg
    57841 gcctgtgcag aagcgggcgt ttttctgatt tcgccgtttg tcgggcgtat ttatgactgg
    57901 tatcaggcac gcaagccgat ggacccgtat gtggtggaag aagatccggg cgttaaatcg
    57961 gtgcgcaata tctacgacta ctataagcaa caccactatg aaaccattgt gatgggcgcg
    58021 agcttccgtc gcaccgaaca aatcctcgcc ttaaccggct gcgatcgact gactatcgca
    58081 ccgaatttgc tgaaggagct gcaggaaaaa gtttcgccag tggtacgtaa attaatcccg
    58141 ccttctcaga cgttcccacg cccagcaccc atgagcgaag cggagttccg ttgggagcac
    58201 aatcaggatg cgatggcggt agaaaaactg tctgaaggca ttcgtctatt cgccgttgat
    58261 caacgcaaac tggaagatct tcttgccgcc aaactataaa ccagccacgg agtgttatat
    58321 gtcccgaaaa gaccttgcca atgcgattcg cgcactcagt atggatgcgg tacaaaaagc
    58381 caattctggt catcccggcg cgccgatggg catggctgat attgccgaag tgctgtggaa
    58441 cgattttctt aaacataatc ctaccgaccc aacctggtat gatcgcgacc gctttattct
    58501 ttccaacggt cacgcgtcga tgctgctcta cagtttgcta catctgaccg gttacgacct
    58561 gccgctggaa gaactgaaga acttccgtca gttgcattcg aaaaccccag gccacccgga
    58621 gattggctat acgccaggcg ttgaaaccac caccggcccg cttggacaag gtttggcgaa
    58681 cgccgtcggg ctggcgatag cggaacgtac actggcggcg cagtttaacc agccagacca
    58741 tgagatcgtc gatcacttca cctatgtgtt tatgggcgac ggctgcctga tggaaggtat
    58801 ttcccacgaa gtctgttcgc tggcaggcac gctgggactg ggcaagctga ttggttttta
    58861 cgatcacaac ggtatttcca tcgacggtga aacagaaggc tggtttaccg acgatacggc
    58921 aaaacgtttt gaagcctatc actggcatgt gatccatgaa atcgacggtc acgatccgca
    58981 ggcggtgaag gaagcgatcc ttgaagcgca aagcgtgaaa gataagccgt cgctgattat
    59041 ctgccgtacg gtgattggct ttggttcgcc gaataaagca ggtaaggaag aggcgcacgg
    59101 cgcaccactg ggggaagaag aagtggcgct ggcacggcaa aaactgggct ggcaccatcc
    59161 gccatttgag atccctaaaa agatttatca cgcctgggat gcccgtgaaa aaggcgaaaa
    59221 agcgcagcag agctggaatg agaagtttgc cgcctataaa aaggctcatc cgcaactggc
    59281 agaagagttt acccgacgga tgagcggtgg tttaccgaag gactgggaga aaacgactca
    59341 gaaatatatc aatgagttac aggcaaatcc ggcgaaaatc gctacccgta aggcttcgca
    59401 aaatacgctt aacgcttacg ggccgatgct gcctgagttg ctcggcggtt cggcggatct
    59461 ggctcccagc aacctgacca tctggaaagg ttctgtttcg ctgaaggaag atccagcggg
    59521 caactacatt cactacgggg tgcgtgaatt tggcatgacc gctatcgcca acggcatcgc
    59581 gcaccacggc ggctttgtgc cgtataccgc gacgttcctg atgtttgttg aatacgcccg
    59641 taacgccgcg cggatggcgg cactgatgaa agcgcggcag attatggttt atacccacga
    59701 ctcaattggc ctgggcgaag atggtccgac gcaccaggct gttgagcaac tggccagcct
    59761 gcgcttaacg ccaaatttca gcacctggcg accgtgcgat caggtggaag cggcggtggg
    59821 ctggaagctg gcggttgagc gccacaacgg accgacggca ctgatcctct caaggcagaa
    59881 tctggcccag gtggaacgta cgccggatca ggttaaagag attgctcgtg gcggttatgt
    59941 gctgaaagac agcggcggta agccagatat tattctgatt gccaccggtt cagagatgga
    60001 aattaccctg caagcggcag agaaattagc aggagaaggt cgcaatgtac gcgtagtttc
    60061 cctgccctcg accgatattt tcgacgccca ggatgaggaa tatcgggagt cggtgttgcc
    60121 ttctaacgtt gcggctcgcg tggcggtgga agcaggtatt gccgattact ggtacaagta
    60181 tgttggtctg aaaggggcaa ttgtcgggat gacgggttac ggggaatctg ctccggcgga
    60241 taagctgttc ccgttctttg gctttaccgc cgagaatatt gtggcaaaag cgcataaggt
    60301 gctgggagtg aaaggtgcct gatggtgatc gccggacgct gattgccgga tgcgacgctg
    60361 acgcgtctta tccggcctac atgtccccgc cattttgttt aacgggtgat ccacaacgtg
    60421 ggccaggcat ctggcccatg ccagttatcg caggtcggtt ctgcggcgta acgcaccagg
    60481 cgaaaacgct gaccgtcaaa gcgccagcgc gcctgaatgc cacaatcgct taatccgcgc
    60541 cctttcgcta aggtcaccag ctcacgcgat ttctcatcaa atgttgcgtt catcagttcc
    60601 agttcattcg tctcctgacc gttgttgaac ggcaaacgca accgaaccgg gcgcgaggcc
    60661 agcggctttt tacgcgacac aatccatgcc aaatcaatgg tgttataggc ccctgcctca
    60721 cagctaatca tcatcagcgc tttatcatca gtcagcgcag tgacattcac ctcacgacgt
    60781 aacggatcaa gcgagcagcg cagaccattc atccgccagt ttccataatc cagcaaatcg
    60841 ttgcgttctt cgagtgagag tggcgtcggc gttgggttaa ccaccgcgac ctctttcagc
    60901 gcaggcgcgg gcggtacgct gagcggcggt tcgtcccctt tcttgatcca ggcggtttca
    60961 ctgccaacgc gcttttgctg agcatcaata aacaacaacg ccgcttttaa gccgctcaga
    61021 gaaatggtct gattgccgtc gcgtaaggtg atcgccttcc cttcctgaat tatctgcaaa
    61081 aacgcggtga tggttgccgt atcatcggtt actaataacc atggtgaaat ccgccacttg
    61141 tcaccactta acgccaacgg ctcaccatct aacagcagcc gtggcgctat ctccccttca
    61201 gacgcatccg gcgacttcaa cccgccgcgc tcaatacgta aaacggcatc ggtatgcgcc
    61261 ccggcgctgc ggctcagggt catcaccagt ccattatgat cgcccgtatt gcgcgccaca
    61321 caaaaatttt gattattaca ggtgacctgc cagtcggaaa acgcccgttg cgctggtgcc
    61381 gcccacacca aagacgtcgg caacaaagcg aaaaaaaaga gaaggaaaat gcgatagcgc
    61441 atggacggta cgaccccaga agttcaaaca aacagcacaa gtcgtatctt cgttgcgcag
    61501 gtagcgcggc tcaatcggat ttgtcggatt gagccaacaa taaacaacga aatattattg
    61561 ttatttttca gtccattaaa tgtgacgttt gcaaatagtt aagcaataac accgtcttac
    61621 cgtcacgtat ctcgccggtt ttgatcatct ccagcgcctg gctgaacggc agctcgagca
    61681 cttcaatatc ttcatcttca acaccgccac cggcgttagc gcgctgattg tcactgtatt
    61741 cggcgataaa aaagtggatt agctcagtca caccgcctgg cgacatatac agttcaaata
    61801 atttgcgcac ttcgcccact tcatagcccg tctcttcaat cgcttctttg cgaatgcaca
    61861 cttccggttc gtcgttatcc agcagcccgg cgcaggtttc aatcagctgc ccgctttcat
    61921 tgccattaac ccaggtagcg acacggaact gacgaatcag aaccacggtc tttttcttcg
    61981 cgttgtacag gaggatcgtc gcaccattac cgcgatcgta aacttcacgt ttatggcgga
    62041 taacttcgcc atctttgcgc gtgagatcgt aggtaatgtt gtgcagggtg aaatagttat
    62101 cggagagaat tttgtcttta atgagggtga tttgttgcgt cataccgact ccacagcgcg
    62161 aaatgaacaa ttatattacg ctgtgaagtc gggtttgtct gcgcaggcta tcagtgagat
    62221 ttgactgatt ttacccccag ccagtcaata atcccctgtg ccgcatgacg tccttctgcc
    62281 attgcggtaa ccaccagatc cgcaccacgc acggcgtcgc caccggcgaa gattttcgga
    62341 ttggtggtct ggtaacggta ctggctttcc acatccgcga tgatgcggcc ccatttgtct
    62401 accgttacac cgtgcgactc cagccacggc atcccgtgcg gattgaagcc aaacgccata
    62461 atcaccgcgt cggctggcat gacaaattca ctgccttcca ccggtactgg atgccgacgc
    62521 ccctgggcat ccggctcgcc aagacgcgta cgcaggaaac gaatcccgca aacgtgacct
    62581 tgttcattca gctcaagcgc caccggctgg acgttaaatt cgaagttggc cccctcttcg
    62641 cgggcgttct tcacctcttt cttcgagcct ggcatgttag cttcatcacg acgataagcg
    62701 caggtgacgt tactcgcgcc gtggcgcagc gcagtacgca cacagtccat cgcggtgtcg
    62761 ccgccgccca gtaccacgac gttaagtccg gcggtattga taaacggctc ttccggtagc
    62821 tcttcgagtc ccatcacctg tttagtgttg gcaatgagga acggcagcgc gtcataaacg
    62881 cccggcgcat cttcattggg taaacccgct ttcatggaac ggtaagtgcc tacgccaacg
    62941 aagaccgcgt cgtattgttc caaaagcgaa tccaaagaga catctttacc cacttcacaa
    63001 ttgagttcga agtgaatccc catcgcgctg aagatttccc gacggcgtgc cagcagggat
    63061 ttatccagtt tgaaagaagg aatgccgaaa gtgagcaagc caccgatttc tggatggcga
    63121 tcgtacaccg tcaccgccac gccattgcgg gtcagaacat ccgcacaggc cagccccgcc
    63181 ggacctgccc cgataatcgc cacccgcttg tccactttgg tgacatgact taagtcagga
    63241 cgccaacctt tcgccaacgc ctgatctgaa atatagcgtt caatgttgcc gatagttacc
    63301 gcaccgtgct catcgcgaat agtacaggca ccttcacaca aacggtcttg cggacaaacg
    63361 cgtccggtaa tttccggcag ggtgttggtc tggtgagaaa gctcgacggc ggcgtcgatg
    63421 tttccggctt tcaccagttc aatccactgc ggaatatggt tatgcagcgg gcaggtccat
    63481 tcacaaacgc tgtgctcgcc gcacttaagg cagcgcgagg cttcccgttg tgcctggtcg
    63541 gcgcgaaatg gcagataaat ttcatcaaaa ccggttttgc gcgcttcaat cgccagttta
    63601 tccggctcgc cacgcgcggg cgttgcctgc atttgttcga ctttactcat taccggcatt
    63661 tcttgcgccg cggtactggc atgccacggt tgatgttcct gacgcgcggt gcgcaagcgg
    63721 cgggatttcg ccatgccgga gagtgcgacg tcagtgacca gttgcagcgc gtccgccggg
    63781 caattctcaa cacaggcagg accgttttcg cgccctgcac aaaggtcgca tttatgcgcc
    63841 gtggctttta cttttcctgc cgcgacgggt gtcaggacga tttgcatcgt accaaacgga
    63901 caggccacca cgcaggattt acagccaata cacttttgct gattgacctg aatgctgtca
    63961 tcaacgtggc tgattgcgcc attagggcag ctacgggcgc agggcgcatc ttcacaatgg
    64021 tgacaggtca ctgcactacg ttgctgttga tgtttgataa ccgtaattcg gggatgaaaa
    64081 tggtgttggc tcaggacatg ttgctcatca ttgtgagcca tgacacaggc gatttcacaa
    64141 gcatgacaac ccagacattg ctgactgttg gccataataa aacgattcat aacgaccttc
    64201 ttttttggtt gtaaaaacct tattctttat atgagtgttg ttattacccg acttacaggg
    64261 gaatcggcaa tgttcatgtg cccagaataa gtaactattt cgctataaac tgtggcagat
    64321 caaataatcc catcactgac taaattgtgt ttcagctaac tggaacatta atgatttttt
    64381 gtggagaaga cgcgtgtgat tgttaaacga cccgtctcgg ccagtctggc ccgggccttt
    64441 ttttacattg tgctgctgtc gattctttcc acgggtatcg ctctgctaac tctggcgagc
    64501 agtttgcgcg acgctgaggc tatcaatatt gccggatcgc tacgtatgca gagttaccgc
    64561 ctgggctacg acttgcaaag tggcagtcca caactcaatg cacatcgcca gttgtttcag
    64621 caggcactgc attcaccggt attaaccaat ctcaacgtct ggtatgtgcc agaagcagta
    64681 aaaactcgct atgcgcatct gaatgccaac tggctggaga tgaataatcg gctcagcaag
    64741 ggcgatttgc cgtggtatca ggccaatatt aataattatg ttaatcagat agacctgttc
    64801 gtgctggctt tacagcacta cgctgaacgc aaaatgctgc tggtggtggc gatttccctg
    64861 gctggcggta tcggtatttt cacgctggtc ttttttactt tgcgccgtat acgccatcag
    64921 gtggttgccc cgctgaatca gctggttacc gccagtcaac gtattgaaca cgggcagttc
    64981 gactcgccgc cgctggatac cagtctgccg aatgagcttg gtctgcttgc aaaaaccttt
    65041 aaccagatgt cgagcgagct gcataaattg tacctttcgc tggaggcgtc agtagaagaa
    65101 aagacccgcg atctccacga ggccaagcgt cgtctggagg tgttgtatca atgttcccag
    65161 gcgctgaaca ccagccagat tgatgtgcat tgtttccgcc atattttgca gattgttcgc
    65221 gacaatgaag cggctgaata tctggagtta aatgtcggtg aaaactggcg gattagcgaa
    65281 gggcaaccaa acccggaatt gccgatgcag attttaccgg tgacaatgca agagacggtt
    65341 tacggcgaac tgcactggca aaatagtcac gtttcatcat cagaaccgct gcttaacagt
    65401 gtttcgtcga tgctgggacg cggtttgtac tttaatcagg cgcagaagca ttttcagcag
    65461 ttattgttga tggaagaacg tgcaaccatc gcccgcgaat tgcacgactc gctggctcag
    65521 gtactttctt acttacgtat ccagttgacg ttactgaagc gttcgatacc ggaagataac
    65581 gccaccgcac aaagtatcat ggccgatttt tcccaggcgt tgaatgatgc ttatcggcag
    65641 ttacgcgagc tgttgactac tttccgcctg acgctgcagc aggcggatct cccctccgcg
    65701 ttgagggaaa tgctggatac gttacaaaat caaaccagcg ccaaactgac cctcgactgc
    65761 cgtctgccaa ccctggcgct ggatgcgcaa atgcaggtgc atttgttgca aattattcgc
    65821 gaagcggtgc tgaatgcgat gaagcacgcc aacgccagcg aaatcgccgt cagttgcgtc
    65881 accgcgccgg acggcaatca cacggtttat atccgtgata acgggattgg catcggtgaa
    65941 ccgaaagagc ccgaaggtca ttatggtctg aatatcatgc gcgaacgcgc ggaacggctg
    66001 ggtgggacgc tgactttttc acaaccttcc ggcggcggca cgttagtgag tattagcttt
    66061 cgctccgcgg agggtgagga aagtcagctg atgtaatgcc tcctactgac caaagaatac
    66121 ttgcacttaa ggttcagtat aaaagggcat gataatttac attaactcct ttttttctcc
    66181 acgattggct cgtaccttgc cgctacagtg aagcaagtca agcctacaac gatacgcaga
    66241 aacacgaggt cctcttttaa tggcgaattt ctttattgat cgccccattt ttgcctgggt
    66301 gctggcaatc ctgttgtgtc tgacaggtac cctggcgatt ttctcattgc ccgttgaaca
    66361 ataccccgat ctcgcgccac cgaatgtgcg agtgaccgct aactatcccg gcgcatcggc
    66421 ccagacgctg gaaaacaccg tgacccaggt tatcgagcaa aatatgaccg gcctcgataa
    66481 tctcatgtat atgtcatctc agagcagtgg caccggtcag gcatctgtca ctttaagttt
    66541 taaagcaggc accgatccgg acgaagccgt gcagcaagta caaaaccagc tgcaatcagc
    66601 catgcgaaag ttaccgcagg cggtgcaaaa tcagggcgtg acggtgcgta aaaccggcga
    66661 taccaatatt ctgaccattg ctttcgtctc taccgatggt tcgatggata aacaggatat
    66721 tgccgattat gttgccagta atattcagga cccgttaagc cgcgtgaatg gcgtcgggga
    66781 tatcgatgcc tatggttcgc aatattccat gcgtatctgg ctggacccgg cgaaactcaa
    66841 cagtttccag atgacggcta aagatgtcac tgatgccatt gagtcacaga acgcgcagat
    66901 tgcggttggg caacttggtg gtacaccttc cgtcgataag caggcgctca acgccaccat
    66961 taacgcccag tcactgctgc aaacaccaga acagttccgc gatatcacct tgcgggtgaa
    67021 tcaagacggc tcagaggtaa ggctgggcga tgtcgccacc gtcgaaatgg gggcggagaa
    67081 atacgattat cttagccgct tcaatggtaa gccagcctcc gggctggggg taaaactggc
    67141 ctccggcgca aacgaaatgg cgacagcgga gctggtgctc aatcgtctcg acgagctggc
    67201 gcagtacttc ccgcacggac tggaatacaa ggtggcgtat gaaaccacct cgtttgttaa
    67261 agcctccatt gaagacgtgg tgaaaacgct gctggaagct atcgctctgg ttttcctcgt
    67321 tatgtatctg ttcctgcaaa acttccgcgc cacgctgata cccactatcg ccgtgccggt
    67381 ggtgttgatg ggaaccttct ccgtacttta cgccttcggt tacagcgtca acaccttaac
    67441 catgttcgcg atggtactgg cgatcggtct gctggtggat gacgccatcg tggtggtgga
    67501 aaacgtcgaa cgtattatga gtgaggaagg actcactcct cgcgaagcca cacgtaaatc
    67561 gatggggcag atccaggggg cactggtcgg gattgcgatg gtgctttcgg cggtatttgt
    67621 gccaatggcc ttcttcggcg gcaccaccgg tgccatctat cgccagttct ctattaccat
    67681 tgttgcagcg atggtgttgt cagtactggt agcgatgatc ctcactccgg ctctgtgtgc
    67741 cacgctactt aagccactga aaaaaggtga acatcacggg caaaaaggct tttttgcctg
    67801 gtttaaccag atgtttaacc gcaacgccga acgctacgaa aaaggggtgg cgaaaattct
    67861 ccaccgtagc ctgcgctgga ttgtgattta tgtcctgctg cttggcggca tggtgttcct
    67921 gttcctgcgt ttgccgacgt cgttcttacc gctggaagac cggggcatgt ttactacctc
    67981 ggtacaattg cccagcggtt caacccaaca acagaccctg aaagtcgttg agcaaatcga
    68041 gaaatattac ttcacccatg aaaaagacaa catcatgtcg gtgtttgcca ccgttggttc
    68101 tggccctggg ggtaacgggc aaaacgtggc gcgaatgttt atccgcctga aagactggag
    68161 cgaacgcgac agtaagaccg gcacctcgtt tgccattatc gagcgtgcaa cgaaggcgtt
    68221 taaccaaatt aaagaagctc gcgttatcgc cagcagcccg ccagcaatta gcggtcttgg
    68281 tagttctgcg ggttttgata tggagttgca ggaccacgct ggagcaggtc acgatgcgct
    68341 gatggcagca cgtaaccagt tgctggcgct ggcggcggaa aacccggagc taacccgtgt
    68401 gcgccataac ggcctcgatg acagtccgca gttgcagatt gatatcgacc agcgtaaagc
    68461 tcaggcgctg ggcgttgcta tcgacgatat taacgacaca ctgcaaaccg cctggggttc
    68521 gagctatgtg aatgacttta tggatcgcgg tcgcgtgaag aaagtctatg tgcaggcagc
    68581 tgcgccgtat cgcatgctgc cagatgacat caatctctgg tatgtccgaa ataaagatgg
    68641 cggcatggtg cccttctctg ctttcgcgac ctcacgctgg gaaacaggct cgccgcgtct
    68701 ggaacgctat aacggttatt ctgcggttga gattgttggg gaagccgcac cgggggtcag
    68761 taccggtacg gcgatggata ttatggaatc gttagtgaag cagctgccaa acggctttgg
    68821 tctggagtgg acggcgatgt cgtatcagga gcggctttcc ggcgcgcagg ctccggcgct
    68881 gtacgccatt tccttgctgg tggtattcct gtgtctggct gcgttgtatg aaagctggtc
    68941 ggtgccgttc tcggtaatgc tggtcgtgcc gctgggggta atcggcgcgc tgctggcaac
    69001 ctggatgcgc gggctggaaa acgacgttta cttccaggtg ggcctgttaa cggtcattgg
    69061 tttatcggcg aaaaacgcca tcctgatcgt cgagtttgct aacgagatga accaaaaagg
    69121 ccacgacctg tttgaagcga cgctccacgc ctgccgtcag cgtttacgcc cgattctgat
    69181 gacctcgctg gcatttatct tcggcgtatt gccaatggca accagcacgg gtgccggttc
    69241 cggtggtcag catgcggtgg gtactggcgt aatgggcggg atgatttcag ctactattct
    69301 ggctatttac ttcgtgccgc tgttctttgt gctggtgcgc cgccgcttcc cgctgaagcc
    69361 gcgcccggaa taagcaataa aaaaggcgac atgccaatgt gtcgcctttt tcaactttcc
    69421 gattaagaac ctgctcagcg ggttcttgct gtttatactt tgtctcggga attacttacg
    69481 aagcataact tcgataaagt ctttccagtt ccccagttca cgttcaatca taacaacctc
    69541 tcttataatt atgggtattc tacggaaaca atataccgtg gtgaagctaa tttactcgat
    69601 tgctgcgatg actacctccg ggggacaaac cttatgtaaa tactatggtc ctgcggtgat
    69661 aatttgtatg tgatacacag caacatttcg agatattcat acggcatcta atactgattt
    69721 aattctggtt aaaatacaga cagataacaa gatgaatatt cttaatgttt acgttaaa
//
LOCUS       DAZGSP010000010        64978 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZGSP010000010 DAZGSP010000000
VERSION     DAZGSP010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.010"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..240
                     /locus_tag="ACHK74_000765"
     CDS             <1..240
                     /locus_tag="ACHK74_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020315086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4209 domain-containing protein"
                     /protein_id="HFP0219631.1"
                     /translation="DNPLIIEAFGVDIIGNLQALLVDKIYGDLRNQLAHGYVPAGYYN
                     QPPCIFAWWLVLHILMNPTARYWQATYGQESEAQT"
     gene            complement(459..860)
                     /locus_tag="ACHK74_000766"
     CDS             complement(459..860)
                     /locus_tag="ACHK74_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP0219632.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKTAVVDPDVIWSLPDG
                     EIRRYDSRLNIICRECRMSEVMQRILAFYQG"
     gene            complement(901..1866)
                     /locus_tag="ACHK74_000767"
     CDS             complement(901..1866)
                     /locus_tag="ACHK74_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219633.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDDGKLSESIGSKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDPTENTT
                     EITTENKNTFRPEASQPDPQTAEQDFLIRHPGAVVFSAKKRQWGSQEDLACAQWIWGR
                     IVGLYEQAASDDGEIMRPKEPNWTVWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            complement(1847..2368)
                     /locus_tag="ACHK74_000768"
     CDS             complement(1847..2368)
                     /locus_tag="ACHK74_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP0219634.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            complement(2352..2579)
                     /locus_tag="ACHK74_000769"
     CDS             complement(2352..2579)
                     /locus_tag="ACHK74_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP0219635.1"
                     /translation="MLKVDAITFFGSKTKLANAAGVRLASVAAWGILVPEGRAMRLQE
                     ASGGELLYDPKVYDEYRKTKRAGRLNNENHS"
     gene            2660..3067
                     /locus_tag="ACHK74_000770"
     CDS             2660..3067
                     /locus_tag="ACHK74_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0219636.1"
                     /translation="MDTRTLGQRVLARRKELRLTQREAARLAGVAHVTISQWERDETQ
                     PVGKRLFALADALKCSPTWLMFGDEDKAPVPAQELHVETELTPNHKELIELFDALPSS
                     EQEALLSEMRARVENFNKLFEEMLKARKNKSIK"
     gene            3236..3391
                     /gene="ydfA"
                     /locus_tag="ACHK74_000771"
     CDS             3236..3391
                     /gene="ydfA"
                     /locus_tag="ACHK74_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP0219637.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            3393..3494
                     /gene="ydfB"
                     /locus_tag="ACHK74_000772"
                     /pseudo
     CDS             3393..3494
                     /gene="ydfB"
                     /locus_tag="ACHK74_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
     gene            4455..4643
                     /gene="dicB"
                     /locus_tag="ACHK74_000773"
                     /gene_synonym="ftsT"
     CDS             4455..4643
                     /gene="dicB"
                     /locus_tag="ACHK74_000773"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP0219638.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            4640..4831
                     /gene="ydfD"
                     /locus_tag="ACHK74_000774"
     CDS             4640..4831
                     /gene="ydfD"
                     /locus_tag="ACHK74_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP0219639.1"
                     /translation="MNSAFALVLTVFLVSGVPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVINQDNIEISAGL"
     gene            4925..7396
                     /locus_tag="ACHK74_000775"
     CDS             4925..7396
                     /locus_tag="ACHK74_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP0219640.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPNETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPVTKQPEPVAQQEVEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMQGAEHPHKENTGGNQHHNSDNETGETADHP
                     IKVNGHHEITSTSRACDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPSQADS"
     gene            7469..7720
                     /locus_tag="ACHK74_000776"
     CDS             7469..7720
                     /locus_tag="ACHK74_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP0219641.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            7755..9035
                     /locus_tag="ACHK74_000777"
     CDS             7755..9035
                     /locus_tag="ACHK74_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0219642.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEEIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
     gene            complement(9223..10243)
                     /gene="rspB"
                     /locus_tag="ACHK74_000778"
                     /pseudo
     CDS             complement(9223..10243)
                     /gene="rspB"
                     /locus_tag="ACHK74_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
     gene            complement(10255..11469)
                     /gene="rspA"
                     /locus_tag="ACHK74_000779"
     CDS             complement(10255..11469)
                     /gene="rspA"
                     /locus_tag="ACHK74_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFP0219643.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(11675..12001)
                     /gene="ynfA"
                     /locus_tag="ACHK74_000780"
     CDS             complement(11675..12001)
                     /gene="ynfA"
                     /locus_tag="ACHK74_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFP0219644.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            12136..12477
                     /gene="ynfB"
                     /locus_tag="ACHK74_000781"
     CDS             12136..12477
                     /gene="ynfB"
                     /locus_tag="ACHK74_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFP0219645.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            12512..13072
                     /gene="speG"
                     /locus_tag="ACHK74_000782"
     CDS             12512..13072
                     /gene="speG"
                     /locus_tag="ACHK74_000782"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFP0219646.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(13075..13785)
                     /gene="ynfC"
                     /locus_tag="ACHK74_000783"
     CDS             complement(13075..13785)
                     /gene="ynfC"
                     /locus_tag="ACHK74_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFP0219647.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            13893..14198
                     /gene="ynfD"
                     /locus_tag="ACHK74_000784"
     CDS             13893..14198
                     /gene="ynfD"
                     /locus_tag="ACHK74_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFP0219648.1"
                     /translation="MKLSTCCAALLLALASPVVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            14397..16823
                     /gene="ynfE"
                     /locus_tag="ACHK74_000785"
     CDS             14397..16823
                     /gene="ynfE"
                     /locus_tag="ACHK74_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFP0219649.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAATV
                     QQASEKVIWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGTRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGITWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVIIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWNSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            16884..19313
                     /gene="ynfF"
                     /locus_tag="ACHK74_000786"
     CDS             16884..19313
                     /gene="ynfF"
                     /locus_tag="ACHK74_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFP0219650.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKVKE"
     gene            19317..19934
                     /gene="ynfG"
                     /locus_tag="ACHK74_000787"
     CDS             19317..19934
                     /gene="ynfG"
                     /locus_tag="ACHK74_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFP0219651.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            19936..20790
                     /gene="ynfH"
                     /locus_tag="ACHK74_000788"
     CDS             19936..20790
                     /gene="ynfH"
                     /locus_tag="ACHK74_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFP0219652.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            20833..21447
                     /gene="dmsD"
                     /locus_tag="ACHK74_000789"
     CDS             20833..21447
                     /gene="dmsD"
                     /locus_tag="ACHK74_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFP0219653.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            21606..22898
                     /gene="clcB"
                     /locus_tag="ACHK74_000790"
     CDS             21606..22898
                     /gene="clcB"
                     /locus_tag="ACHK74_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFP0219654.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(22851..23546)
                     /gene="bioD"
                     /locus_tag="ACHK74_000791"
     CDS             complement(22851..23546)
                     /gene="bioD"
                     /locus_tag="ACHK74_000791"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP0219655.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(23671..24891)
                     /gene="mlc"
                     /locus_tag="ACHK74_000792"
                     /gene_synonym="dgsA"
     CDS             complement(23671..24891)
                     /gene="mlc"
                     /locus_tag="ACHK74_000792"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFP0219656.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSCLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(25026..25919)
                     /gene="ynfL"
                     /locus_tag="ACHK74_000793"
     CDS             complement(25026..25919)
                     /gene="ynfL"
                     /locus_tag="ACHK74_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0219657.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFEPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQRPAARNFRIHLLNALR"
     gene            26026..27279
                     /gene="ynfM"
                     /locus_tag="ACHK74_000794"
     CDS             26026..27279
                     /gene="ynfM"
                     /locus_tag="ACHK74_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0219658.1"
                     /translation="MSRTTTVDGASASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(27328..27447)
                     /locus_tag="ACHK74_000795"
     CDS             complement(27328..27447)
                     /locus_tag="ACHK74_000795"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFP0219659.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            27703..28011
                     /gene="asr"
                     /locus_tag="ACHK74_000796"
     CDS             27703..28011
                     /gene="asr"
                     /locus_tag="ACHK74_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFP0219660.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            28104..28187
                     /locus_tag="ACHK74_000797"
     CDS             28104..28187
                     /locus_tag="ACHK74_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219661.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            28287..29108
                     /gene="ydgD"
                     /locus_tag="ACHK74_000798"
     CDS             28287..29108
                     /gene="ydgD"
                     /locus_tag="ACHK74_000798"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFP0219662.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(29147..29476)
                     /gene="mdtI"
                     /locus_tag="ACHK74_000799"
     CDS             complement(29147..29476)
                     /gene="mdtI"
                     /locus_tag="ACHK74_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFP0219663.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(29463..29828)
                     /gene="mdtJ"
                     /locus_tag="ACHK74_000800"
     CDS             complement(29463..29828)
                     /gene="mdtJ"
                     /locus_tag="ACHK74_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFP0219664.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            30240..31274
                     /gene="tqsA"
                     /locus_tag="ACHK74_000801"
     CDS             30240..31274
                     /gene="tqsA"
                     /locus_tag="ACHK74_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFP0219665.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(31299..32687)
                     /gene="pntB"
                     /locus_tag="ACHK74_000802"
     CDS             complement(31299..32687)
                     /gene="pntB"
                     /locus_tag="ACHK74_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFP0219666.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(32698..34230)
                     /gene="pntA"
                     /locus_tag="ACHK74_000803"
     CDS             complement(32698..34230)
                     /gene="pntA"
                     /locus_tag="ACHK74_000803"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFP0219667.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPLIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            34754..35698
                     /gene="ydgH"
                     /locus_tag="ACHK74_000804"
     CDS             34754..35698
                     /gene="ydgH"
                     /locus_tag="ACHK74_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFP0219668.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            35884..37266
                     /gene="ydgI"
                     /locus_tag="ACHK74_000805"
     CDS             35884..37266
                     /gene="ydgI"
                     /locus_tag="ACHK74_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0219669.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAYAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            37303..38025
                     /gene="folM"
                     /locus_tag="ACHK74_000806"
     CDS             37303..38025
                     /gene="folM"
                     /locus_tag="ACHK74_000806"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFP0219670.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(38022..38357)
                     /gene="ydgC"
                     /locus_tag="ACHK74_000807"
     CDS             complement(38022..38357)
                     /gene="ydgC"
                     /locus_tag="ACHK74_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFP0219671.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            38486..39205
                     /gene="rstA"
                     /locus_tag="ACHK74_000808"
     CDS             38486..39205
                     /gene="rstA"
                     /locus_tag="ACHK74_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFP0219672.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLSKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            39209..40510
                     /gene="rstB"
                     /locus_tag="ACHK74_000809"
     CDS             39209..40510
                     /gene="rstB"
                     /locus_tag="ACHK74_000809"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFP0219673.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            40586..41515
                     /gene="tus"
                     /locus_tag="ACHK74_000810"
     CDS             40586..41515
                     /gene="tus"
                     /locus_tag="ACHK74_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFP0219674.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGARVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(41512..42915)
                     /gene="fumC"
                     /locus_tag="ACHK74_000811"
     CDS             complement(41512..42915)
                     /gene="fumC"
                     /locus_tag="ACHK74_000811"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFP0219675.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAASKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(43058..44704)
                     /gene="fumB"
                     /locus_tag="ACHK74_000812"
     CDS             complement(43058..44704)
                     /gene="fumB"
                     /locus_tag="ACHK74_000812"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP0219676.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            44903..46078
                     /gene="manA"
                     /locus_tag="ACHK74_000813"
     CDS             44903..46078
                     /gene="manA"
                     /locus_tag="ACHK74_000813"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFP0219677.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            46179..47687
                     /gene="ydgA"
                     /locus_tag="ACHK74_000814"
     CDS             46179..47687
                     /gene="ydgA"
                     /locus_tag="ACHK74_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFP0219678.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(47913..49178)
                     /gene="uidC"
                     /locus_tag="ACHK74_000815"
     CDS             complement(47913..49178)
                     /gene="uidC"
                     /locus_tag="ACHK74_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFP0219679.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGMGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(49217..50590)
                     /gene="uidB"
                     /locus_tag="ACHK74_000816"
     CDS             complement(49217..50590)
                     /gene="uidB"
                     /locus_tag="ACHK74_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFP0219680.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(50587..52398)
                     /gene="uidA"
                     /locus_tag="ACHK74_000817"
     CDS             complement(50587..52398)
                     /gene="uidA"
                     /locus_tag="ACHK74_000817"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFP0219681.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(52787..53377)
                     /gene="uidR"
                     /locus_tag="ACHK74_000818"
     CDS             complement(52787..53377)
                     /gene="uidR"
                     /locus_tag="ACHK74_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFP0219682.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(53606..54373)
                     /gene="hdhA"
                     /locus_tag="ACHK74_000819"
     CDS             complement(53606..54373)
                     /gene="hdhA"
                     /locus_tag="ACHK74_000819"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFP0219683.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(54485..55513)
                     /gene="malI"
                     /locus_tag="ACHK74_000820"
     CDS             complement(54485..55513)
                     /gene="malI"
                     /locus_tag="ACHK74_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFP0219684.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            55688..57280
                     /gene="malX"
                     /locus_tag="ACHK74_000821"
     CDS             55688..57280
                     /gene="malX"
                     /locus_tag="ACHK74_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFP0219685.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            57290..58462
                     /gene="malY"
                     /locus_tag="ACHK74_000822"
     CDS             57290..58462
                     /gene="malY"
                     /locus_tag="ACHK74_000822"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFP0219686.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            58566..59567
                     /gene="add"
                     /locus_tag="ACHK74_000823"
     CDS             58566..59567
                     /gene="add"
                     /locus_tag="ACHK74_000823"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFP0219687.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(59602..60642)
                     /gene="ydgJ"
                     /locus_tag="ACHK74_000824"
     CDS             complement(59602..60642)
                     /gene="ydgJ"
                     /locus_tag="ACHK74_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP0219688.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            60885..61010
                     /gene="blr"
                     /locus_tag="ACHK74_000825"
     CDS             60885..61010
                     /gene="blr"
                     /locus_tag="ACHK74_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFP0219689.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            61283..61498
                     /gene="ydgT"
                     /locus_tag="ACHK74_000826"
     CDS             61283..61498
                     /gene="ydgT"
                     /locus_tag="ACHK74_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFP0219690.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            61584..62024
                     /gene="ydgK"
                     /locus_tag="ACHK74_000827"
     CDS             61584..62024
                     /gene="ydgK"
                     /locus_tag="ACHK74_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFP0219691.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            62101..62682
                     /gene="rsxA"
                     /locus_tag="ACHK74_000828"
     CDS             62101..62682
                     /gene="rsxA"
                     /locus_tag="ACHK74_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFP0219692.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            62682..63260
                     /gene="rsxB"
                     /locus_tag="ACHK74_000829"
     CDS             62682..63260
                     /gene="rsxB"
                     /locus_tag="ACHK74_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFP0219693.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            63253..>64978
                     /gene="rsxC"
                     /locus_tag="ACHK74_000830"
     CDS             63253..>64978
                     /gene="rsxC"
                     /locus_tag="ACHK74_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFP0219694.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQ"
BASE COUNT        16413 a        16540 c        15695 g        16330 t
ORIGIN      
        1 gataatccac tgattattga agctttcggc gtagacataa ttggcaatct tcaggcctta
       61 ctggtagata aaatctatgg tgatttacga aatcaactag ctcacggata tgtgcctgcc
      121 ggttactaca atcaacctcc ctgcattttt gcatggtggc ttgtattgca cattctaatg
      181 aaccctacgg cgagatactg gcaagccact tatggccaag agagtgaggc tcagacttaa
      241 agtgaataag tttacgaagt tggcacttcc ggatgcatgt ccatatgagt tgaggcgatt
      301 tggttaataa ggttgagtgc tgctgaaatt attcgatgtc cgcttttggc acaaagcgga
      361 caaccacgcc agatctaccc tgtgccatga aaaatgagaa tgtcaattca ctcctgaact
      421 aatgcttttt aatctagtaa cgtctaaaat acctaacatt atccctgata aaatgccagt
      481 atgcgctgca taacttcact catccggcac tcgcgacaga ttatgtttag gcgactgtcg
      541 tagcgacgta tttctccatc aggtaacgac cagataacgt ccggatcaac cacagctgtt
      601 ttcttcacct ttgccctcga gagttttttg cgggcgtttt gccagtcctt acgcgcctgt
      661 tcagacggga ataacccgta gccggagttg tatacatcgc cactggctac cagctctctg
      721 gcaagaacgc tcatcagata tctagtcgca cctgttttag cttccagttg ccgtaacgtc
      781 tcacgaccgc tctggcgcac gagttccacc acctgccctt taattttttc ccgctcttct
      841 tgtgtaaaaa cttttgccac aagtccccct taaaattacc tcatgacctg aaatcaaaac
      901 ttatcctctg aaaccaggcg gaatttctgt atccggttca gaaatatgat taacacaacg
      961 ctgtacaggt gaacgcccca ggcggatgac cagttcgtcc catttttcgc ggagctttga
     1021 cgggctcatg atgtttttta cccagaatgg atcccgctgt acccgaccaa acatttcaca
     1081 aatttgtctg tggcttctgc catccagcat ccgcattgtg cgcacatcat tggcccagac
     1141 agtccagtta ggctcttttg gtcgcatgat ctcgccatca tcactggcgg cctgttcgta
     1201 gagacccacg atccgccccc atatccactg cgcacacgcc agatcctcct ggctacccca
     1261 ctggcgtttt ttcgcactaa acacaactgc gccagggtgc cggattaaaa aatcctgttc
     1321 agccgtctgc gggtccggtt gcgaagcttc cggacgaaaa gtgtttttat tctctgtagt
     1381 aatctctgtt gtattctctg taggatcatc gggccatttt gacccgatga cattgggtcg
     1441 ttttgaacca atggagcgtt tcattttgac ctcttccatc gtgtcatttt gacctgatgg
     1501 agcggcgcat tttgaaccga tggattcgct caatttgcca tcatctaaaa gctcgctccc
     1561 atagttaatc gtgtagaaat tggtcatatc gcgctttgat ttattgagct tttcacaacg
     1621 caaaagcccc agcgttttca gacttgcaaa cgcgcgcttt aacgttgact ctgaccagaa
     1681 tgggaactgt tccaaccatt gttccgttgt gttgtaaatc cagcgaacac catcacattc
     1741 catgccggaa ttggtatctc tcaaccagta atgcaactgc tgcaacacaa tggcttcatt
     1801 taagccaatc ttcatcgcaa gctgtgtgtt tataaccagt gggcgttcag caaaaagaag
     1861 gctcataatt ccatccagct ttttgttggt attgctgtcg atacgcaagt ttgaaagcaa
     1921 ttgctttttc tataagttcg tcagtttcac gatccactac ggcaggatca gcaaaaagca
     1981 gtccggactc caccacatcg ccatattctt tgtttaaccc ggcgatcatg tacgtgatgc
     2041 tttttccgtc actaatttca cgatacaacc tgaaatcatt aatccggata gcctccataa
     2101 ttgcaggcac tagcgccgtg aacttttcac gcttatccct ggtgtcgata gccttccagc
     2161 gttcgaatat cttcactcga ttaacgccaa gcgctcgctg atcaaccgcg ccaccttcat
     2221 ctgtgacacg ctgaacatcg atgttcgggc gctctttcaa agcccagaat gcttcagtga
     2281 ttaatatcgt cgcctgctcc tgtgtcattc ctggtcgaca tatccaggca tccagagcct
     2341 cacgagcctg ttcaggagtg attttcattg ttcaaccgcc ccgcccgctt cgtcttacga
     2401 tattcgtcat aaaccttggg atcatacaga agctccccgc cagatgcctc ctgtagacgc
     2461 atcgcgcgac cttcaggaac cagtatcccc caagcagcaa cacttgccag tctcactcct
     2521 gcggcattgg caagctttgt tttgctgcca aaaaaagtaa ttgcgtcaac tttaagcatc
     2581 aaagccccct cttgttagac ttttctaaca ttattgtgcg cgggatatct aagtcaagaa
     2641 aaattagaat tacctaacta tggatacaag aaccctaggc cagcgagttc tggcgcgacg
     2701 aaaagaatta cgcttaacac aacgagaagc tgcgcgcctc gctggagttg ctcacgtcac
     2761 aatttcacaa tgggaaagag acgaaaccca gccagtcgga aaacgattgt ttgctttagc
     2821 ggatgctctg aagtgctcac ctacatggct aatgtttggt gacgaagaca aggcaccagt
     2881 gcctgcacaa gaacttcatg tggaaacaga gctaactccc aaccacaaag aattgatcga
     2941 attattcgat gctcttccat cttccgagca ggaagccttg ctgtctgaaa tgcgcgcaag
     3001 agtagaaaac ttcaacaaac tcttcgaaga aatgcttaaa gcgcgtaaaa ataaatcaat
     3061 aaaataacat tcttttcaag tgattagttg cgcccactct ttttgttaga ctaatctaac
     3121 aaaaaacact tgcctctcat gttaggttat tctaaattac tttccatcaa gacaccgcac
     3181 ggtgttctca gcaaacagtt ccgctacccc ggcgttaagg ggaaatgagg tcagcatgga
     3241 tactatcgat cttggcaaca gcgaatctct ggtatgtggc gtgttcccca accaggacgg
     3301 cacgttcacc gcgatgacgt ataccaaaag caaaacgttt aaaaccgaaa atggtgcccg
     3361 tcgctggctg gaaagaaact caggtgagtg atatggattt cgacacaatc atggaaaagg
     3421 cttacgaaga atacttcgaa ggccttgccg aaggcgaaga agctctcagc ttcagtgagt
     3481 ttaaacaggc actcagaata agaatgtgct ctcacaatga cgcggagcac aaatatgaga
     3541 agcaaaatca gaccgcagaa aattttgttc tggaacccgg agaaacgctt ttcaaaattc
     3601 ccgttacgtg ccccatttgc ggttttacat cagaagaact tgacgactcc tgtaacaatc
     3661 aggaaacaac caagtatgtc gaagatgata ccgagtgcgc acgaagaacg attatatcca
     3721 cgagtccaaa ctccagggcc aataaatctc actttgagag ggtgattaat ccactccccc
     3781 aaaccaataa aaaagatgcc ggaggacaaa aaacccaatg gaacaacggg atatctgtca
     3841 aaaaaagacg ttccattaaa gacaaacaac gcagcgccaa caactgtaag cgctttatac
     3901 cagtaatcaa ttttcatgtt cttaagcgga tttattggtg gttgcgacat tgcttaatga
     3961 atccttaaaa ctgtggtgat tttaaggata ccacctcgcc tgacgtggtt aaaagcaggc
     4021 acacaacacg aaagcgcacg gcgaagttcg tctcactgta cggtgtcgtt aaatttaatt
     4081 cgaccgtgcg cttccggttg tggcaacccg caaaatggcg cggcggtaag tatggcgggg
     4141 ttattccttc cccgttgagg acaccgggtt gtcaggttga ccatacgctt aagtgacaac
     4201 cccgctgcaa cgccctctgt tatcaatttt ctggtgacgt ttggcggtat cagttttact
     4261 ccgtgactgc tctgccgccg tttttaaagt gaattttgtt atgcggtgaa tgcggctaag
     4321 cgcacgcgga acagttaaaa ccaaaaacag tgttatgggt ggattctctg tatccgacgt
     4381 taattgttaa ctggttaacg tcacctggag gcaccaggct ctgcatcaca aaatttattg
     4441 ttgaggacgc gataatgaaa acgttattac caaacgttaa tacgtctgaa ggttgttttg
     4501 aaattggtgt cactatcagt aaccctgtat ttactgaaga tgccattaac aagagaaaac
     4561 aagaacggga gctattaaat aaaatatgca ttgtttcaat gctggctcgt ttacgtctga
     4621 tgccaaaagg atgtgcacaa tgaattcagc atttgcgctt gttctgacag tttttcttgt
     4681 ttccggagtg ccagttgata ttgcagtcag tgttcacagg acaatgcagg agtgtatgac
     4741 tgcagcaacc gaacagaaaa ttcccggtaa ctgttacccg gtcgataaag ttattaacca
     4801 ggataatatc gaaatctcgg caggtcttta aaacagttcc gtaataaata tccggtttca
     4861 ttcttatatg ccagcaatgg cagggatttg ttcatcctta aatctgtcat gaggttaaaa
     4921 caaaatgagt aaagtcttta tttgcgccgc tattcctgac gaactggcaa caagggaaga
     4981 aggcgctgtg gctgtagcca cagccattga agctggcgac gaacgccgtg ctcgagcaaa
     5041 atttcactgg caattcctgg aacattatcc ggctgctcag gactgcgctt ataaatttat
     5101 tgtctgcgag gataaacctg gcataccccg ccctgccctc gattcatggg atgctgaata
     5161 tatgcaggaa aaccgctggg atgaggagtc tgcttctttt gtcccgaatg agactgaatc
     5221 cgatccgatg aacgtcactt ttgacaagct ggcccctgaa gtacagaacg ctgtcatggt
     5281 taagttcgac acatgtgaaa acatcaccgt tgatatggtt attagcgcac aggaattgtt
     5341 gcaggaagac atggcaacat tcgacggaca tatcgttgaa gcgttgatga aaatgccaga
     5401 agttaacgcc atgtatccgg agcttaagtt gcacgccatt gggtgggtta agcataaatg
     5461 tattcctggt gctaaatggc ccgaaattca ggcagagatg cgcatctgga aaaaacgtcg
     5521 cgaaggtgaa cgcaaggaaa ccggaaaata cacgtctgtt gttgatctcg cacgcgccag
     5581 agccaatcaa cagtacactg aaaattcaac aggaaaaatc agcccggtca ttgctgccat
     5641 tcatcgcgaa tacaagcaga catggaaaac actggatgac gaactggcct acgctctctg
     5701 gcctggtgat gtggatgccg gaaacattga cggcagcatc catcgctggg caaaaaaaga
     5761 agttatcgac aacgaccgcg aagactggaa gcgtatctcg gcatcaatgc gcaaacagcc
     5821 tgatgccctt cgctacgacc gccagactat ttttggcctt gtccgtgagc gtccgatcga
     5881 cattcacaaa gatcccgtag cactgaacaa atatatctgc gaatacctga cgacaaaggg
     5941 cgtgtttgag aatgaagaaa cagacctggg cactgttgat gttctccagt catcagaaac
     6001 acaaactgat gcagtggaaa ctgaggtatc tgatatccca aaaaatgaaa ccgcgccgga
     6061 agctgaacca tctgtagagc gtgaggggcc gttctatttc ctcttcgcag ataaggacgg
     6121 agaaaaatac ggtcgcgcaa acaaactttc tggtctggat aaggcactgg ctgctggcgc
     6181 cactgaaatc acaaaagaag aatattttgc ccgaaaaaat ggcacataca cgggcttacc
     6241 gcaaaatgta gataccgctg aagattcaga acaaccagag ccgataaaag ttaccgctga
     6301 cgaagtaaac aaaattatgc aggcagccaa tatcagccag cctgacgctg ataagttgct
     6361 tgctgcatca cgtggtgaat ttgttgaagg gattagtgac ccgaatgatc cgaaatgggt
     6421 taaggggatc cagacccgcg attctgtgaa ccagaaccag catgaatcgg aacggaacta
     6481 ccaaaaagcg gaacaaaaca gtccaaatgc gttacaaaac gagccagtaa cgaaacagcc
     6541 tgaaccagta gcgcaacagg aagtggaaaa agtctgcacc gcctgcggtc agaccggcgg
     6601 cggcaactgc cctgattgtg gcgcggtgat gggcgacgca acataccagg aaacattcga
     6661 tgaagagtat caggttgaag ttcaggaaga tgatccggag gaaatgcaag gcgctgaaca
     6721 tccacacaag gagaacactg gcggcaatca gcatcacaat agcgataatg aaactggcga
     6781 gacggcagat cacccaatta aggtgaacgg tcatcacgaa atcacatcca ccagcagggc
     6841 gtgtgaccat ctaatgatcg accttgaaac catgggaaaa aatcctgatg ccccgatcat
     6901 ctcaataggt gcaatatttt tcgatccgca aaccggagat atgggaccgg aatttagtaa
     6961 gactatcgat ctggaaactg ctggcggagt cattgatcgg gacaccatta aatggtggct
     7021 taagcaatca cgcgaagcgc aatctgccat tatgaccgat gaaatcccgt tagatgatgc
     7081 actgttacaa ttgcgggaat ttatcgacga aaactccggt gaattttttg ttcaggtttg
     7141 gggaaatgga gccaacttcg acaacacgat tttgcgccgt tcatacgaac ggcaggggat
     7201 cccctgcccg tggcgttact acaacgatcg cgatgtacgc acaatcgttg agctggggaa
     7261 agccatagac ttcgatgcca gaacggctat tccattcgaa ggtgagcgcc ataatgcact
     7321 tgatgacgcc cgttaccagg caaaatacgt ttcagttatc tggcaaaaac tgatcccgag
     7381 tcaggctgat tcttaatgtt caactgtcgc cggttgtgac tggtattctg caaccggcgc
     7441 tcgtctgatg taagagataa agaaatcgat gagcgaagta atcatgattg tctctcccgg
     7501 caaatgggta tccgaagagc agttaattgc gctgaaagga ataaaaaaag gtacgttaaa
     7561 aaaggcccgg gaaaaatcgt ttatggaagg aagggaatat aagcatgtcg ctcatgacgg
     7621 tatgccatgg gataacagtc catgctttta caacctggaa gaaattgatc gctggattga
     7681 gcgccaggca tcagcgagac caagacgtca tcttacttga ctaaaagcca cactaactaa
     7741 tgagagaagt tgaaatgaaa tatccgacag gcgtggaaaa ccatggaggg aaattacgta
     7801 tctggtttgt ttataaaggc gtaagagtca gggaaaatct gggggttcct gacacagcaa
     7861 aaaacaggcg cgttgcaggt gaactacgct cctctgtttg ttacgcaata aaaactggtg
     7921 ttttcgacta tgcaaaacag tttccctcct cacgcaatct ggaaaaattt ggtgaggccc
     7981 gacaagattt aaccataaaa gaactggctg aaaaatttct ggcactgaaa gaaactgaag
     8041 tcgccaaaac atcactcaac acataccgtg ccgtcatcaa aaatatcctg agcataatcg
     8101 gtgaaaaaaa tcttgcctca tcgattaata aagaaaaatt actggaggtt cgtaaagagt
     8161 tactgactgg ataccagatc cccaaaagta actatattgt tacacaacca gggagatcgg
     8221 ctgtaactgt aaataattac atgacaaatc ttaacgccgt gttccagttt ggtgttgata
     8281 acggttacct ggcagataat ccgtttaagg ggatctcgcc attaaaggaa tcaagaacca
     8341 ttccggatcc tctttcgcgg gaagaattta tccgtcttat cgatgcgtgc agaaatcagc
     8401 aagcaaaaaa tttatggtgt gtttctgttt atactggcgt tcgccctggt gagctgtgtg
     8461 cacttggatg ggaggaaata gatctgaaaa atggaacaat gatgatcagg agaaatttag
     8521 caaaagaccg tttcacggta ccaaaaacac aggcgggaac caatcgggtc attcatctta
     8581 ttaagccagc aatcgacgct ctccggagtc agatgacatt aacgagactg agcaaagagc
     8641 atatcattga tgttcacctc agagagtatg gcagaacaga aaaacaaaaa tgcacctttg
     8701 tttttcaacc tgaagtgtca gcgagagtaa aaaattatgg tgaccatttt accgttgact
     8761 caataaggca gatgtgggac gcagcgataa aacgtgccgg actccgccat cgaaaatcat
     8821 atcagtcgag acatacttat gcctgctggt cgctgacagc tggtgctaac ccggcattta
     8881 tagcaaacca gatgggccat gcagatgcgc aaatggtatt tcaggtatac ggaaaatgga
     8941 tgtctgaaaa caataatgca caggtagctt tgttaaatac acagttaagc gagtttgccc
     9001 caaccatgcc ccataacgaa gcaatgaaaa attaatttaa tatttatcaa atagttaaca
     9061 cgcatgactc ttgaaatcca taaattcaag cgcagtgccc agccatcccg atactgctgc
     9121 tttcaccaaa tccttagtgc ttctttcatg tttttctatt gtcataatgg ttatctctaa
     9181 aaaagaggta agatgcgtac tacttactcg ccgttattgg tattattcag aaaaagtgag
     9241 taagactttg cagcaatgtt tttgatcctg ttcaaataaa ctaatggcat cagcaacatg
     9301 ctggaaatca aacgtatggg taattaattt ttctggttta attaaccctt tacttaacca
     9361 gtcgataaca accggaaatt tatttgcatt taagcgtgaa gagaaaatag agagttcttt
     9421 tccggtaatt ccttgctgaa tcacttcaga cggttcactg gagaagccca tcaatacaat
     9481 acgtgccgca ggagaagcca gcgttacggc ctctttcagg atagaaggat gacaagccgc
     9541 atcgataatt aatgtcggct tgatgccttt ttcagcgaaa atctcgccaa gcggtgtctg
     9601 gctgttatta atcgcccagt ctgcccccgc tctctttcgc tttttccagt cgttcatcaa
     9661 tgcgatcggc aacaatcaca tttttaacgt tatagacgcc ttttaatacc tgaacgatcg
     9721 tcaggccgat tggaccggca ccataaacca gaacggtatc attttcagtc ggttgaccat
     9781 gaccggtaac gttagccgca atggtaaaag gttcgatcat caccgcatat tgatcggcca
     9841 ctgcttcagg aattttccac gcattttttg ccggaaccac ggcatattca ctgaaaccac
     9901 cgtcagcgtg cacacctaat acagcaagtg tcgtacaaac gttcggctta cctatagagc
     9961 acggatagca atgcccacag ctgaccaccg gatcgacagc aacgcgttca ccgactctgg
    10021 cgctttccac accttcaccc accgcatcaa tgacgccaaa gaattcatga ccaatgacgc
    10081 gcggatattt cgcaaaagga ttatgcccac ggtaaatatg gctatctgaa ccacaaattc
    10141 cggcaagttt cacttttact cgtacttcac ccgctgacgg ggtgggtatt tcacgttcga
    10201 taatcgacag ttgattcggt ttttcaatta aaatgctttt cattacctta ctccttacca
    10261 gttccacagc gtgccatctt ccagacgtgc gactggcaga taagcaggtt catagggata
    10321 tttcgccgcc agcttttcat cgaattcgat gccaagaccc ggtttgtctc ccggatgcat
    10381 atagccgtta tcgaaagtcc agttgtgcgg gaagacttcg agcatttgtt cggaataacc
    10441 catgtactcc tggacaccga aattggggac ccacagatca aagtgcagcg ccgcagccat
    10501 gcagactggt gacaaatcgg aaggaccgtg tgagccagta cgtacctgat acagcgaagc
    10561 aaaatcggca atccggcgca taccggtaat tccgcctgca tgggtcagcg tggtgcggat
    10621 ataatcgatg agttgctctt caatcagttg tttgcagtcc cagatgctgt tgaagacttc
    10681 acccactgcg atgggtgtga cggtatgttg gcgaatgaga cggaagcatt cctggttttc
    10741 cgcaggcgtc gggtcttcca tccagaacat gcgataatct tcaatgcttt taccaaagcg
    10801 cgccgcttca ataggcgtta agcgatggtg catgtcatgc agcaaatgtt cattaaaacc
    10861 aaacttgtta cgtaccgcgt caaacaattt cggcatgaaa tcgaggtatt tctccgtcga
    10921 ccacagctgc tcttccggcc actgtccttt ggttgcgggt tcataagcca gacctttacc
    10981 tttcgacatg ccgtaggtgg ttttcatacc agggattccg cactgcacgc ggatggcttt
    11041 gaatcccagc tcctgatgac gggcataatc atccagagct tcatcaatac tgtgaccggt
    11101 ggtatggcaa taaaccatca ccccttcacg agacgcgccg ccgagtaact ggtaaagcgg
    11161 catgttggca gctttggctt taatatccca cagcgccata tcaaccgctg aaatggccga
    11221 catcgtaacc ggaccgcgac gccagtaagc acctttatag aaaaactgcc agatatcttc
    11281 gatacggtgc gcatcgcgac caataagctg cggacaaagg tgatcctgca aataagaggc
    11341 cacggaaagc tcacgtccat tgagggtggc atccccaagg cccgtaatac cgtcctcagt
    11401 ggtgattttt aatgtgacga aattacgccc cggacaggta acaaaaactt cagcctttac
    11461 gatcttcatg ttcgattcct tgcatcgctt gtcgtgatgc atgaaatcta cgcaactgag
    11521 ctactaccat acaagtataa agatcgaaaa aagccggagt gatcacaaaa aaaggcgtat
    11581 atttgcgctg tgaatggttg acaaaagatg aaatagaata ccttttgtca gctgacactt
    11641 cctcttatct tattgataaa atggatttat gttcctacgt gcgcccccag cccgcaacaa
    11701 tgatcaacat gccgcaaagc gcaatcaacg cacccgtcca gtcataaaga gtcagtttca
    11761 cgccatccac aacgcgcagc cacatcaacg ccgtgcagac ataaacgcca ccataagccg
    11821 cgtaaacacg cccactcgcc gctggatgca acgttaacaa ccagacaaac agcgccagtg
    11881 aaatccccgc cggaagcaac agccagatac tggcgtttcg ttttaaccac aaccagggca
    11941 gaaagcatcc aataatttca cacagcgcag tagcaaaaaa tagtaacgtt gttttaatca
    12001 tctttgtctc ttattgacat catgtatagt tatagggcga cataatatca tcaatataaa
    12061 caccctcctg gtacgttttg cgtccgcagt ggatggtgta gaatcacctt taatcattca
    12121 tacagggaat gaattatgaa aatcactctc agcaaacgaa tcggcctgct cgctattctg
    12181 ctgccttgcg cactggcatt gagcacaact gttcatgccg aaactaacaa actggtgatt
    12241 gagtctggcg acagtgcaca aagccgccag cacgccgcta tggaaaaaga gcaatggaat
    12301 gacacgcgca atctgcgcca gaaagtgaat aaacgcactg aaaaagagtg ggataaagcc
    12361 gacgccgctt ttgataaccg cgataaatgt gagcaaagcg ccaacatcaa tgcctactgg
    12421 gagcccaata ctttgcgctg cctggaccgt cgaactggcc gcgttattac cccctaacct
    12481 gttattgatt taaggaatgt aaggacacgt tatgccaagc gcccacagtg ttaagctacg
    12541 cccgctggag cgtgaagatt tacgctatgt acatcaactc gacaacaacg ccagtgtgat
    12601 gcgttactgg tttgaggaac cctacgaagc ctttgttgaa ctctctgatc tgtatgataa
    12661 gcatattcac gatcagagcg aacggcgctt tgtggtggaa tgtgacggcg aaaaagccgg
    12721 tctggtggag ctggtggaaa ttaaccatgt tcatcgccgc gcagaatttc agataattat
    12781 ctccccggag tatcagggga aaggtctggc aacccgtgcc gccaaattag caatggacta
    12841 tggctttacc gttctcaatc tctataagct gtatctgatc gttgataaag agaatgaaaa
    12901 agcgattcac atttaccgca agcttggctt ttcggttgaa ggtgaattga tgcacgagtt
    12961 ctttattaat ggtcaatatc gtaatgccat tcgcatgtgt atattccagc atcagtatct
    13021 ggcagagcac aaaacaccgg gtcagactct cctgaagccg accgcacaat agcattaata
    13081 ataatcgatc gtatttttga tggtgtaaac ccgttcgacg gcgggtttta ctccttcatc
    13141 aacaatgatt agctgacagt ccaccggatt agcgtgactg tcatattcac agctctgttt
    13201 tacattacca acccgttgat tattcagtaa agtaaccgct gtgtaatcta attttttgat
    13261 cggatccgtt gatggcgtgg cgctgacaga taatgttttg tcgttacttt tcgtggtttt
    13321 acccagcgga taaccctgat catcatagcg atattccatc tgcatttgtt tgctgctggc
    13381 tttaatcacg aagccattat catcggtttc ccaactcacc ccggcagaag gtaattctgc
    13441 tagctggcat ttaccctgta aacgtactct cttctccagc gtctcggcat cacggtaata
    13501 attggcgtcc agtaccagag cgaccacggt attattttcc agatccagta attcgagtga
    13561 atcaaaacag ccttcttccg acaaagtccc agaaacacgt ttcgtcactt caccttgctc
    13621 atccattaat gtctgagtga aatcttttac cggaccacgc agcggatcaa aatcgaattc
    13681 attagagaaa ctggccatct caggggtaaa tgaaagtgtt acctctgtgc ggtcacatcc
    13741 tgtgaggaat atcgcgagta agcatggtag taatttgtat ttcacaacag tcaccagaga
    13801 gtagagatga ttctcaatca tagtagcaaa tacagtactt tacacgttaa atgctatgct
    13861 taaagaagtt atcttcgcgt aaggagctta tgatgaaact ctcaacctgc tgtgccgcgc
    13921 ttctgctcgc cctcgcctca cccgtggtac tcgccgcgcc gggatcctgt gagcgcatac
    13981 agagcgatat atcacagcgc attatcaata atggcgtacc cgaaagcagc ttcacgttaa
    14041 gtattgtacc caatgaccag gttgatcagc ctgattccca ggtcgtcggc cattgcgcta
    14101 atgatacgca taaaattctc tatacccgca ccaccagcgg taacgtctct gctcccgcgc
    14161 agtctaccca ggatggcgcg cctgccgaac cgcagtaata cattatcgac tgaacgccgg
    14221 atacgacaca tccggcgatt tgaacataca acataatccc accttattac tcataccctt
    14281 ctattgatat ggattaataa ttcttaaccc aaaatgggta gactccctct attgttagcg
    14341 cgctaaatat tcaatatata aacatttata taacgataaa gaacagggag tgagttatgt
    14401 ccaaaaatga ccgaatggtg ggcatcagcc gcagaacact cgttaaatca accgcgatag
    14461 gttctctggc gctggctgca ggcggttttt ctttaccgtt caccctgcgc agtgcagcgg
    14521 caacggtaca acaggccagc gaaaaagtga tctggggtgc ctgttccgtc aactgtggta
    14581 gccgctgtgc acttcgtcta catgttaaag ataatgaagt gacctgggtg gaaactgaca
    14641 ataccggcag cgatgagtac ggcaaccatc aggtacgcgc ctgtttgcgc ggtcgctcca
    14701 tccgccggcg tattaatcat cccgatcgct tgaattaccc aatgaaacgc gtgggtacac
    14761 gcggtgaagg caaattcgaa cggattagct gggatgaagc cctggatact atcgccagta
    14821 gcctgaaaaa aaccgtcgaa caatatggca atgaggctgt atatattcag tactcttcgg
    14881 ggattgttgg cggcaatatg acccgctctt cgccatcagc ctcggcggtc aaacgcctga
    14941 tgaactgcta cggcggttca ctcaatcagt atggctccta cagcaccgcg caaatttcct
    15001 gtgccatgcc ctacacctac ggcagtaatg atggcaacag caccacggat attgaaaaca
    15061 gcaagctggt cgtgatgttt ggtaacaacc cggcagaaac ccgcatgagc ggcggtggca
    15121 tcacttatct tcttgaaaaa gcgcgcgaga aatcgaacgc caaaatgatt gttatcgatc
    15181 cgcgatatac cgatacggct gctggtcgtg aagatgaatg gctccctatt cgcccgggca
    15241 ccgatgccgc gctggttgcg ggtattacct gggtattgat taacgaaaat ctcgttgatc
    15301 aaccttttct cgataaatac tgcgtcggct atgacgaaaa aaccttaccc gcagatgcac
    15361 ccaaaaatgg tcactataaa gcctatattc ttggtgaagg tgatgataac acagcgaaaa
    15421 cgccgcagtg ggcttcgcaa attaccggta tcccggtaga ccgtatcatc aaactggcgc
    15481 gtgaaattgg cacagcaaaa cccgcttata tctgccaggg ctgggggccg caacgccagg
    15541 caaacggcga actgactgca cgcgctattg ctatgttacc tattttgacg ggcaatgtcg
    15601 gcatcagcgg cggaaatagt ggcgcgcgtg aatcgaccta taccattacc atagaacgcc
    15661 tgccggtgct ggataatccg gtcaaaacgt caatctcctg cttcagctgg acggatgcta
    15721 tcgatcatgg tccgcaaatg acggcaatcc gcgacggcgt ccgcggcaaa gataaactgg
    15781 atgtgcccat taagttcatc tggaactacg cgggaaatac cctcgttaat cagcattctg
    15841 acatcaacaa aacgcatgaa attctgcagg acgaatcgaa atgcgaaatg attgtgatca
    15901 tcgaaaactt tatgacctca tcggcaaaat atgccgacat tctgctgcca gacctgatga
    15961 ccgttgagca ggaagatatt attcctaacg actacgccgg taacatggga tatctcattt
    16021 tcctccagcc tgtcaccagc gaaaaattcg aacgcaaacc gatttactgg atcctgagtg
    16081 aagtcgcgaa acgtcttgga cctgacgtct atcaaaagtt cacagaaggt cgcacgcagg
    16141 aacaatggtt acaacatctg tacgccaaaa tgcttgccaa agatccggcg ttaccgtctt
    16201 acgacgaact gaaaaaaatg ggtatctata agcgtaaaga tcccaatggc cattttgtcg
    16261 cctacaaagc atttcgtgac gaccccgagg caaatccact taaaacgcct tccggtaaga
    16321 ttgaaattta ttccagcagg ctggcggaaa ttgcccgtac ctgggaactg gaaaaagatg
    16381 aagtgataag cccattgcca gtttatgcct caacctttga aggctggaac tcccctgaac
    16441 gtagaacctt cccactgcaa ctgtttggtt tccattacaa atcccgtact cactcgacct
    16501 acggcaatat tgatctcctg aaggctgcct gccgtcagga ggtgtggatc aaccctatag
    16561 atgcgcagaa acgtgggatt gccaacggcg atatggtgcg ggtgtttaac catcgtggcg
    16621 aagttcggct accagccaaa gtaacaccac gtattctccc tggagttagc gctatgggcc
    16681 agggagcctg gcacgaggcc aatatgtctg gcgataaaat cgaccatggc ggctgtgtga
    16741 atacgctaac cactctgcgc ccttcaccac tggcgaaggg aaacccgcag cacactaatc
    16801 tggtcgagat cgaaaaaata taacccacga caaccataaa ttctggcatg acattttgtt
    16861 tgaaaagcaa taagtgagta atgatgaaaa tccataccac agaggcgctc atgaaggctg
    16921 aaatcagccg tagaagtctg atgaaaacct ccgcacttgg cagtcttgcg ctggcaagca
    16981 gtgctttcac tctgccattt tcccaaatgg tccgggcggc agaggctccg gtagaagaga
    17041 aagcggtctg gagttcctgc accgttaact gcgggagccg ctgtctgtta cgtttgcatg
    17101 tgaaagatga caccgtgtac tgggtggagt ctgatacgac aggtgacgac gtctacggta
    17161 accatcaggt tcgagcgtgt ttacgcgggc gctctatccg ccgacggatg aatcatcctg
    17221 ataggttgaa atatcccatg aagcgcgtcg gcaagcgcgg tgaaggtaaa tttgaacgga
    17281 taagttggga cgaagccctg gataccatca gtgataatct tcggcggatc ctgaaagatt
    17341 acggcaatga ggctgtacat gtcctgtacg gaacaggcgt agatggcgga aacatcacca
    17401 actcaaacgt cccgtaccgt ctgatgaact cttgcggtgg ttttctcagt cgctatggca
    17461 gctacagtac cgcacagatc agtgccgcaa tgagttatat gttcggtgcc aatgatggca
    17521 acagcccgga tgatatcgcc aatacgaaac tggtcgttat gttcggaaat aacccggcag
    17581 aaacgcggat gagcggcggt ggtgtcactt actacgtcga gcaagcccgc gaacgttcaa
    17641 acgcacgcat gatcgtcatc gatccacgtt ataacgacac tgctgccggg cgtgaagatg
    17701 aatggctgcc cattcgccct ggcaccgatg gcgcactggc ctgtgcgatt gcctgggtac
    17761 tgattactga aaacatggtc gatcagccat ttctcgacaa atattgtgtt ggttacgatg
    17821 aaaaaacgct gcccgccaac gcaccacgta acgcgcatta taaagcctat attctgggcg
    17881 aagggcctga cggcatagct aaaacgccgg aatgggcagc aaaaatcacc agcatcccgg
    17941 cagaaaaaat tatccagttg gcacgagaga tcggttcagc aaaacctgct tatatttgtc
    18001 agggttgggg gccacaacga cattccaacg gcgagcaaac gtcccgcgct attgccatgc
    18061 tttccgttct caccggtaac gtcggcataa acggcggcaa ctcaggtgta cgcgaaggta
    18121 gctgggatct gggggtagaa tggttcccga tgcttgagaa tcctgttaaa acgcagattt
    18181 ccgtctttac atggacagat gccatcgacc atggtacgga aatgaccgcg acccgtgatg
    18241 gtgttcgtgg aaaagaaaaa ctggatgtcc ccatcaagtt tttatggtgc tacgccagta
    18301 acacattgat caatcaacat ggcgacatca atcacaccca tgaggtgctt caggatgaca
    18361 gcaagtgcga gatgattgtt ggcattgacc acttcatgac ggcctcggct aagtattgcg
    18421 atatcctgtt gcccgacctg atgccaacag agcaagaaga ccttatctct catgaatctg
    18481 cagggaatat gggctatgtg atcctcgccc aacccgcaac ctcagcaaaa tttgaacgca
    18541 aacccatcta ctggatgctg agtgaagtcg ccaaacgctt aggaccagac gtttatcaaa
    18601 cctttactga aggtcgcagt cagcatgaat ggatcaaata tctccatgcg aaaacgaagg
    18661 aacgtaaccc tgagatgccc gactacgagg agatgaaaac gaccgggatc tttaagaaaa
    18721 aatgcccgga agaacactac gtcgctttcc gcgcattccg tgaagatcca caggcaaacc
    18781 cgttgaaaac accttcgggg aaaatcgaaa tttattctga acgactggcg aagattgcag
    18841 atacctggga attgaaaaaa gatgaaatta ttcatcccct tcctgcgtat accccaggtt
    18901 ttgatggctg ggacgatccc ctgcggaaaa cctatccact gcagttaacg ggcttccact
    18961 acaaagcgcg tacccactcc agctacggca atattgatgt gttacagcag gcctgcccac
    19021 aagaggtgtg gatcaacccc attgatgctc aggcacgcgg tatccgtcat ggcgataccg
    19081 tgcgggtatt taacaataat ggagaaatgc tgattgccgc aaaagtgact ccgcgtattc
    19141 tgcctggcgt taccgccatc gggcaaggtg cgtggcttaa agcggatatg tttggtgacc
    19201 gggtcgatca cggcggcagt atcaatattc tgacctctca ccgcccttca ccgctggcaa
    19261 agggaaaccc gtcgcacagc aatcttgtcc agatcgaaaa ggttaaggag tagcccatga
    19321 ccacacaata tggatttttt atcgattcca gccgctgtac tggctgtaaa acctgcgaac
    19381 tggcgtgcaa agattttaaa gatcttggcc cggaagtcag tttccgccgt atttatgaat
    19441 acgctggcgg cgactggcag gaagataatg gcgtctggca ccagaacgtg ttcgcttatt
    19501 atctctccat ctcctgtaac cattgcgatg accccgcatg tacaaaagtc tgccccagcg
    19561 gcgcaatgca taagcgtgaa gatggctttg tggtggttga tgaagatgtt tgtatcggct
    19621 gccgctactg ccacatggct tgtccgtacg gcgcaccaca gtacaatgct gaaaaagggc
    19681 acatgacgaa gtgcgatggt tgttattcgc gcgtcgccga ggggaaacaa cccatatgtg
    19741 tcgaatcctg cccgctgcgt gcacttgagt tcggtccaat tgaagagctg cgccagaagc
    19801 acggcactct ggctgccgtc gcgccactgc cgcgcgcgca tttcacaaaa cccaatatcg
    19861 ttatcaaacc taacgccaac agccgcccga ccggcgatac cactggttat ctggctaatc
    19921 cggaggaagt gtaaaatggg aaatggatgg catgagtggc cactggttat ctttaccgta
    19981 ttgggtcaat gcgtagtagg cgcgctgatt gttagcggaa ttggctggtt cgccgccaaa
    20041 aatgacgcag accgtcagcg tattgtccgt ggcatgtttt tcctctggct gttgatgggc
    20101 atcgggttta ttgcctctgt catgcacctg ggatcgccac tgcgagcgtt taactcgctg
    20161 aatcgaatcg gagcttctgg tctgagtaac gagattgctg ccggttccat attttttgcc
    20221 gttggcggac tatggtggct ggtggcggtc atcggtaaaa tgccgcaagc gttggggaaa
    20281 ctctggttac ttgtcagcat ggcgctcggc gtcattttcg tctggatgat gacctgcgtg
    20341 tatcaaatcg acaccgtgcc aacctggcat aacggttaca ctacgctggc tttcttcctg
    20401 acggtattgt tgagcggtcc aatacttgct gccgcaattc tgcgggcagc tcgcgttaca
    20461 tttaatacca caccatttgc catcattagc gttttggcgc tgatcgcttg tgccggggtg
    20521 attgtgctgc aaggtttgtc tctggcgtct attcactcat ccgtgcaaca agccagcgca
    20581 ctggtaccag attacgcctc cttacaggtc tggcgtgtgg tattgttatg cgcaggtctt
    20641 ggatgctggc tttgcccgct cattcgtcgc cgtgaacctc acgttgccgg acttattctg
    20701 gggctaatcc tcatacttgg cggtgagatg atcgggcgtg tgctctttta tggcctgcac
    20761 atgacagtag gaatggctat tgcaggttaa caaattggtg cggggcaacc cgcacatttc
    20821 aggatgttag gaatgaccca tttttcacag caagataatt tttctgtcgc ggcgcgcgtg
    20881 ttgggcgcgc tgttttatta cgctccagag agtgcggaag ccgcacctct ggttgcggta
    20941 ctcaccagtg acggctggga aactcagtgg cctctaccag aagcgtcatt agcgccgctg
    21001 gtgacggctt ttcagacaca gtgtgaagag actcacgccc aggcctggca gcgtttgttt
    21061 gtcggcccgt gggcactgcc gtctccgccg tgggggtcgg tctggctgga tcgcgaatct
    21121 gtgctgtttg gcgattcaac attggcactt cgtcagtgga tgcgcgagaa aggcattcag
    21181 tttgaaatga agcaaaacga accggaagat cattttggtt cactgctgtt gatggctgcg
    21241 tggttggcgg agaatggtcg ccagacagaa tgcgaagaat tgctggcatg gcaccttttt
    21301 ccgtggtcaa cacgttttct tgatgttttt atcgaaaaag cagaacaccc tttctaccgt
    21361 gcactgggtg aactggctcg cttaacgctg gcgcaatggc aatcacaact gttaattcct
    21421 gtcgcggtta aaccgctgtt tcgataggat caccgtaata ttgccggatg gtgacgatta
    21481 cgcgcaatcc ggcaataata ggttacagtg tcacgttttt ttatctctta aagcacgcac
    21541 tgcttttgcg gctggcctct tttgccgcaa aatagtcgcc cgtgtttcat tgcccatttc
    21601 tgctcatgca tcatctacac atctatccgg atctgcgcac tatgttccgc cgtctgctta
    21661 tcgcaacagt cgtcggtatt ctcgcggcct ttgccgttgc cgggtttcgt catgcgatgc
    21721 tgctactgga gtggttgttc ctcaataatg actccggcag tctggtcaat gcagcgacaa
    21781 acctttcccc ctggcgacgg ttgctaactc cggcgctcgg cggactggcg gcgggtttgt
    21841 tgctgatggg ctggcagaaa tttacccaac aacgccctca tgcgccgacc gattacatgg
    21901 aagcgttgca aaccgatgga cagttcgatt acgcagcaag cctggttaaa tcgcttgcct
    21961 cgctgctggt agtaaccagc ggcagtgcaa ttggtcgcga aggtgcgatg attcttttag
    22021 ctgcccttgc cgcctcctgt tttgcccaac gttttacgcc acgccaggag tggaaattat
    22081 ggatcgcctg tggggccgcg gcgggaatgg ctgcggccta tcgtgccccg cttgctggca
    22141 gtttatttat cgccgaagtg ctgtttggca ctatgatgtt ggcctctctc ggcccggtga
    22201 ttatttccgc cgtcgtggca ttgctggtta gcaatctgat taatcatagc gacgcgttac
    22261 tctacaacgt acaactctca gtgacggttc aggctcgtga ctatgcgctg attatcagta
    22321 caggtgtgct ggcaggtctg tgcggaccac tgttgttaac gttaatgaac gcctgccatc
    22381 gtggatttgt gagtctcaaa cttgcgccgc cctggcaact ggcactaggc gggttgatcg
    22441 tgggtctgct ttccctgttc acacctgcag tgtggggcaa cggctatagc accgtacaat
    22501 cctttttaac cgccccacca ctgttaatga tcattgccgg gatcttcctt tgtaaactgt
    22561 gtgccgtgct ggcgagtagc ggttccggcg cacccggtgg ggtctttaca ccgacgctat
    22621 ttatcggtct tgccattggc atgttgtatg gtcgtagcct gggattatgg ttccctgatg
    22681 gcgaagaaat tacactttta ctcggattga ccgggatggc gacactgttg gcggcgacca
    22741 cgcacgcgcc gattatgtcg acgttgatga tatgtgaaat gaccggggag tatcagctac
    22801 tccccggttt attgattgcc tgcgtaattg cgtcggtaat ttcgcggacg ttacaccgtg
    22861 actctatcta ccgccagcac actgcgcagc atagctaagc ggatgtattg ccccagttca
    22921 cgctgttcgg cgcgcggcag ataaggcagt tcaccaatga gcggtgccgg aagtttttta
    22981 cccagcacat caatgatttc cgcataatgc gccagtcctg ggttgattcg gttagccacc
    23041 cagccaatga gcggcagccc gtcgttggcg atcgcctgag ctgttagcag tgcatggtta
    23101 atgcaacctt cctgaatacc gacaaccatc aacaccggca gttgttcctg cactacccat
    23161 tcagagagtg gacgcaaatc attcatcaga ctgcgccagc cgccagtccc ttctaccacg
    23221 acatgatcga ctttttcggt caggtttgcc aggccgtttg aaatgagggt gtaattgatt
    23281 gggcaactgt gcgccacgct actttcttct tcgcttaacg cgataggatt aactgcttca
    23341 taaggcagtt cgatggttga aacactctgc aacaccaggg catctttatt acgcagccct
    23401 tcgggtgtct ctttgctccc cttcgctacg ggtttatatc ccgcaaccgt ttttccctgg
    23461 gaggctaacg cttgtagcaa tgcgcgggaa accaccgttt tccctacaga agtgtctgta
    23521 ccggtaataa agaaacgctt cagcatcact aactccaccg ttatgcttca caaatataaa
    23581 ccaggaaaat aattaacctt gaaagtctaa gttatgcttt cctggcccaa attgagatag
    23641 cgcaaatttt ggtagaagag ttaaaaaatg ttaaccctgc aacagacgaa tcaacaaaga
    23701 accgttatac atcgcgtctt ttaccagtgc agcccctgcc atcgtgccct ggttagaaaa
    23761 ctgagtactc tcaacgctga tgtgctgact atacgcagga agggcctgct gacggatgct
    23821 gtctgagatg accgggaaga ggatatctgc cgctttactt aacggtgagc caatcagtat
    23881 tttttgtggg ttaaataaat tcaccatgat ggcaagaatg cgccctacat gcgcgcccac
    23941 cccggtaatg atgtcttttg ccagcagatc gccgcgcaat gccgcctgac acaatgagtc
    24001 cacggttaac ggttgtccat gtaacatcga gctcatggat tgattaagac gcagctgtgc
    24061 cagctcaaga atactgtcca cactggcgat ggtttcgagg cagccgtgat tcccgcaata
    24121 acagcgtttc ccatacgggt cgacctgtgt gtggcctatt tccacgagac tactgctgcc
    24181 tgcgtgtagc agatgaccat cggtaatgac gcccgccccc acgttgtgat cgataaccac
    24241 ctgaatcaca tcgcgcgccc cgcgtgaggc accaaacaag gcctctgcca tcgtccatgc
    24301 gctgatatca tgctgaatat aaaccggtac gccggtatgc tgctccagcg cctcgccgag
    24361 cggcatctct tttacatcct cgtagaacgg catacgatgt acaataccat tttccgtatc
    24421 aataattccc ggcaaggtta tggcaatcga agttagacgc tcaagttttt tctggtggcg
    24481 aataaaaaac tgatcgatat gggaaatgat acgatccagc aatggcgagt catcttttaa
    24541 cgccagttcc tgcgactctt ccaccaccag tttgctgctc agatcgcgca gagcaaggaa
    24601 aatctccccg cgactaatgc gcagagaaag atagtgccag gcttcagttt caaccaccag
    24661 ccccaccgcc ggacggccac ggttccccgc ttctttgatt tccagctctt gcaccaggtg
    24721 tgcttcgagc atctcacgga caattttagt gatactggca ggagccagtt gcgccagaca
    24781 ggaaagatcg atacgcgaga ctggaccaag ctgatcaatc aggcgataaa ccgcgcccgc
    24841 gttggtctgc tttatttgat caatgtgccc aggctggttt tcagcaacca ccgcatactc
    24901 cctatatttt cgcgctccga aataatctgt aggctatggt gaagcacttc aatacgtgtc
    24961 gtcaaatttt tacttaggca tgtgattaac agcacatttt tcgggctttt tcgctgaaat
    25021 ttccctcacc tgagagcatt cagcagatga atacgaaagt tacgcgcagc tggtctttgt
    25081 tcatgatgtt tcggccagac caaccacatt tcagaaaccg catcctcttc tgcaatcggc
    25141 acccagcgca tttcgttgag ctgaacacgt ttaaatgacg caggcaaaat tgaaacaccc
    25201 agaccggcgg aaaccagacc gatgatggtc attgcctcgc ccacctcctg agtgatgacg
    25261 ggcgtcaaat ggtaacgtcg catcagcccg agaatatcgt catacagccc tgtcccgacg
    25321 tgcggctcaa aaaagacgaa gggttctttc gccagttcag ccagcgttac attcgggtta
    25381 tttgccaggg gatgatcgtg cgggatcatc gccataagcg gttcatggac gatgactgcg
    25441 tgttcaagcg tctccggtaa cgctgtgtta cgcagcaatc ccatatccag cgttccttca
    25501 atgagcggag cgatttgctc acgagtgttc atttcgcggg tttgtaaatg gacatcggga
    25561 taatcacggc gaaacagcga taacgtatcg gacaccgccc gaataaaagg agcggacgaa
    25621 gtaaaaccaa tacgcaactc ccccgcttca ccctgatgca gtctttcagc gcgagcggca
    25681 gcgtcatcca ccatagacag gatttgccga ctatctgcaa gaaactgttt tcctgctgcc
    25741 gtcagcaata cactgcgatt ggttcgtgcc agcagtcggg caccaatttg ttgctccagc
    25801 gcctgaatct gctgacttag aggtggttgc gaaatattca ggcgggcagc ggcgcgcccg
    25861 aaatgcagct cttccgcaac agcaacaaag taacgcagat gacgaagttc aatattcata
    25921 tttaaaacat cttatttgag attattaata tattagacag aacaattcga ttttcctacc
    25981 ctatgtataa gcctgatcca caggcatatt tagcaaggat ttcaagtgag ccgtactaca
    26041 actgttgatg gcgcttcggc aagcgacact gacaagcaaa gcatttctca gccaaatcaa
    26101 tttattaaac gcggtacgcc gcaatttatg cgcgtcaccc tggcgctgtt ctctgccgga
    26161 ctggcaacct ttgcacttct ctattgtgtg cagcctatcc ttccggtgct ttcgcaggag
    26221 tttggcttaa cccccgcgaa cagtagtatt tcactgtcca tttccacggc gatgttggct
    26281 attggtttgc tgtttactgg ccccctatcc gatgccattg gtcgcaaacc agtgatggtc
    26341 acggcgctac tgttggcctc catttgtacg ttactttcga caatgatgac cagctggcac
    26401 ggcattttga ttatgcgcgc cttgattggg ctttcgttaa gtggcgtggc agctgttggc
    26461 atgacttatc ttagcgagga aatccacccc agtttcgtgg ccttttcgat ggggttgtat
    26521 atcagcggca actcaattgg cggcatgagc ggacgcttaa ttagtggtgt cttcacggac
    26581 tttttcaact ggcgaattgc tctggcggca atcggttgtt tcgcgctggc ctcggcgttg
    26641 atgttctgga aaatccttcc ggaatcacgc cattttcgcc cgacttcgct gcgccctaag
    26701 acgttattta tcaactttcg tctgcactgg cgtgaccggg gattaccgtt attgttcgca
    26761 gaaggctttt tgctgatggg gtcgttcgtc acgctgttta attacatcgg ctatcggttg
    26821 atgctttccc cctggcatgt cagtcaggcc gtggttggct tattatcgct ggcttatttg
    26881 accggtacat ggagctcacc caaagccgga accatgacca cccgctacgg gcgcggtccg
    26941 gtgatgttgt tttcgacggg ggttatgctg tttggtttac tgatgacctt attcagctcg
    27001 ctgtggctga tctttgccgg aatgttactc ttctcagcag gattcttcgc tgcccactca
    27061 gttgccagca gctggattgg cccccgcgca aaacgcgcta aaggccaggc ctcctcgctg
    27121 tatctgttca gttactatct ggggtcgagt attgccggga cgctgggtgg tgttttctgg
    27181 cataactatg gctggaacgg cgtcggcgca tttattgctc tgatgctggt cattgctctg
    27241 ctggtcggga cgcgtttgca tcgtcgtctg cacgcctgaa aaataagccc ggactgcggt
    27301 aactacacgt ccggacttat tgcttgctca aaccaacgtt aatagccatc ctaaaataga
    27361 cgaagcgcca gccaattccc gcagcgcgtc tagcgtcatc aggattataa gtacccaaat
    27421 aaacggattc attttgctgt gtgtcattta ttactgatgc gcagttattc taccgctttg
    27481 taagtagtaa aatagttaac ccgatcaaga ctactattat tggtagctaa atttccctta
    27541 agtcacaata cgttattatc aacgctgtaa tttattcagc gtttgtacat atcgttacac
    27601 gctgaaacca accactcacg gaagtctgcc attcccaggg atatagttat ttcaacggcc
    27661 ccgcagtggg gttaaatgaa aaaacaaatt gagggtatga caatgaaaaa agtattagct
    27721 ctggttgttg ccgctgctat gggtctgtct tctgccgcct ttgctgcaga gactgcgacc
    27781 acacctgctc cgactgcgac gaccaccaaa gcagcgccgg cgaaaacgac acatcataaa
    27841 aaacagcata aagcagcacc tgcccagaaa gcgcaggcgg ctaaaaagca tcataaaaat
    27901 acgaaagctg aacagaaagc ccctgaacaa aaagcgcagg cagcgaagaa acacgccggg
    27961 aaacacagcc atcagcaacc ggcaaaacct gctgcacaac ccgcagcgta agttttcaac
    28021 agtaatgctg gcgcgccccc tcgcgcctga aaattacggt gctaagcggg taacgtttag
    28081 caccgccttt agcggagggt agtatggtgg gccgttatcg ctttgagttc attcttatca
    28141 tccttatttt atgcgcactg attgccgccc gtttttatct ttcctgactg tagttatctg
    28201 attttactcc cactttcatc ccgtcccgtc tatagtattt atgagggttt gcttttaata
    28261 atcataatta cccaccagag tgtgatatgc gtacaaccat tgctgtagtg ttgggtgcaa
    28321 ttagtttgac gtctgctttt gtgtttgcag ataaaccaga cgttgccaaa tcggcaaacg
    28381 atgaggtcag caccctgttt tttggtcatg atgatcgtgt gccagtgaat gacacgaccc
    28441 aatcaccgtg ggatgcggtt gggcaactgg aaacggccag cggcaattta tgtacggcga
    28501 cgctgattgc acccaatctg gcattaacgg caggacactg tttattgaca cctccaaagg
    28561 gtaaagccga taaagcagtg gcgctgcgtt ttgtgtcaaa taaaggtctt tggcgctatg
    28621 agatccacga catagaaggc cgcgttgatc cgacactggg aaagcggtta aaagcagatg
    28681 gggatggttg gattgttcct cccgcagctg caccatggga cttcggcttg attgtgctgc
    28741 gtaatccccc ttctggcatt acaccgttgc cgttatttga gggagataaa gccgcgctta
    28801 ctgccgcatt aaaatcggct ggtcgtaaag tgactcaggc gggatatcct gaagatcatc
    28861 tcgatacgtt atacagtcat caaaactgtg aagtgactgg ctgggcgcaa acgtcggtga
    28921 tgtcacatca gtgcgatacc ttgccgggtg acagcggttc gcctctgatg ttgcataccg
    28981 atgacggctg gcaattaatt ggggtgcaaa gttcggctcc tgccgcgaaa gatcgctggc
    29041 gcgccgataa ccgggccatt tctgttaccg gttttcgcga caagctggat caactgtcgc
    29101 aaaaataatg ttcaaacgct gcccgacagc gcgggcagcg tcttcatcag gcaagtttca
    29161 ccatgatcat tccagccaac agcaagacca ggccaatcca gcctttacga tttaaccgtt
    29221 gaccaaacaa gatccaacct gcggctaacg tggcggcaat accaaacccg ccccacaatg
    29281 cataagcgac agacaagtcg atccctttta cggcctgaga aagcgcacta aaggcagcca
    29341 gcaccgccgc cagggagagc aagccaaata ttttgcgacg aaagccgtca gaaaatttca
    29401 aaaagacgtt agcaacgatt tccagcacga ttgccaatgc cagccaggcg gcgtgaaccc
    29461 attcaaactg cgccatggtt cacctccagt tcaggtttac gcgctttacg ggtacctgat
    29521 ttgatcaaca caatcccggc gaccagggtg gttaacccgg caattttcat cagcgataaa
    29581 ctttcgtcga ataacaaaac gctaaacaag gtaataaata aaataccgat accttcccac
    29641 agcgcataag ctacgcctaa ggcgatcttt ttaacggcga aagagagaaa tatatacgac
    29701 agagaaatca tcaccagcat taaaataaag ccgccatttc cctcactgac gctcgcccat
    29761 ttcattgaca gcgtaccggt aatttctgta gcaatagcca ggcctaataa aatccaataa
    29821 atatacattg tccttctcct gcaagagaat tattttaatt ttcgcttaat tcagcgaaac
    29881 caaagtaaag tggtcaagcc ccgcgcatag ggcaagctta ggcagaagaa aggactaaag
    29941 cgcgttgcgc cagtgctgct cacctacgag caagatagtg gatgaggtac gaagatgggt
    30001 aaatgttgaa aataacgtcc tgaacaaatt gtccataata ttacaattat ccgcagtgtt
    30061 gcttctcgtc atcgcggatg ataattgtcc tcggtagttg aacacgcctg atttgtatca
    30121 tagcttaaga attaactcaa aatattttca cttctttacc tgagcggttt gattttcgtt
    30181 atgatgacgg agcgaaaaag acattattat tagcaaagga agaaaaaacg gggacaagca
    30241 tggcaaagcc gatcatcacg ctcaatggcc taaaaatcgt cattatgttg ggaatgctgg
    30301 tcattattct ctgcggtatc cgttttgccg ccgagatcat cgtgccgttt attctcgcat
    30361 tatttattgc tgttattctt aacccgctgg tgcaacacat ggtccgctgg cgagtgccgc
    30421 gtgtactggc ggtgtcgatt ttgatgacca tcatcgtgat ggcgatggtg ttgctattag
    30481 cttatctggg ttccgcgctc aacgagttga cgcggacgtt accgcaatat cgcaactcta
    30541 ttatgacgcc gctgcaagct cttgagccgt tgttgcaacg cgtagggatt gacgtctcag
    30601 ttgaccagct ggcgcattac attgatccga acgcggcgat gacgttgctc accaacttat
    30661 tgacgcagtt atctaatgcc atgtcatcaa tatttttatt gctgctgacg gtgctgttta
    30721 tgctgctcga agtgccacaa ttgcccggaa aatttcagca aatgatggcg cgtccggttg
    30781 aagggatggc ggcgattcaa cgtgcaattg acagtgtttc tcattatctg gtgctgaaaa
    30841 cagccatcag catcatcacc ggcctggtcg cctgggcgat gctcgccgca ctcgatgttc
    30901 gcttcgcttt tgtctgggga ttgctggcct ttgcgcttaa ttacatccct aatattggtt
    30961 cagttctcgc ggcaatcccc cctatcgctc aggtactggt gtttaatggc ttctacgaag
    31021 cgttgctggt gctggcggga tatctgctga ttaatctggt cttcggcaat attctggagc
    31081 cgcgcatcat gggacgtggg ctggggcttt ccacattggt ggtatttttg tcgttgattt
    31141 tttggggatg gttgttagga ccggtgggta tgctgctttc cgtgccgtta acaattattg
    31201 tcaaaattgc gcttgaacaa acagcgggag gtcaaagcat cgccgttctg ttaagcgatc
    31261 tcaataaaga gtgacggcct ccgcagaggc cgtcagggtt acagagcttt caggattgca
    31321 tccacgctgg ctttggcgtc accaaacagc atgtgggtgt tttccttgaa gaacagcggg
    31381 ttttgcacac cagcatagcc agtgttcatc gaacgtttaa agacaatcac gttctgcgct
    31441 ttccacactt ccagcacagg cataccagca ataggactct tcggatcatc ctgcgccgcc
    31501 gggttaaccg tatcgttagc accaatcacc agtacggtat cggtatcagc aaagtcatcg
    31561 ttgatctcgt ccatttccag cacgatgtca tacggtactt ttgcttcagc cagcaatacg
    31621 ttcatatgtc caggcaaacg ccccgcaacc gggtggatac cgaaacgcac gttgatacca
    31681 cgagcgcgca gtttctcggt aatttcagcg acaggatatt gcgcctgcgc gactgccatg
    31741 ccgtaccccg gagtaatgat cactgaatgg gagtttttca gcagttccgc tgtctcttct
    31801 gcggtgattt cgcggtgctc gcccacttcc tgatcatcgc cagtagaaga gccgtcggtg
    31861 ccgaaaccac ccgcaataac gctgataaag gaacggttca tcgccttaca cataatgtaa
    31921 gaaaggatag cgcccgaaga accgaccagc gcaccggtca caatcagcag gtcgttgctg
    31981 agcataaagc ccgcagccgc agccgcccag ccggagtacg agttcagcat cgacaccacc
    32041 actggcatat ctgcaccacc gatggaggcg actaaatgcc agccgaatac cagcgcaatg
    32101 gcggtcatta tcagcaatgc cagcacttgc aggccgacgc tatccgtgcg aacaaataca
    32161 atcagcagca ggaaggaaac gaccagagcc gccaggttca ttttgtgacg gtttggcagc
    32221 atcaacggtt tagacgaaat cttgccacac agtttgccga acgccaccac cgaacccgtg
    32281 aacgttaccg ccccgatgaa gataccgagg aacacttccg tcaggtgaat attgaccaga
    32341 atcggtgcca ttcccgcgtc atgatgcaga taactgttaa agccaaccag cactgccgcc
    32401 agacccacga agctatgcag gatcgccacc agttctggca tttcggtcat ttcaactttc
    32461 ttcgccagac ggataccaat tgccccacca atgaccatcg ccagcaagat ccagccaaca
    32521 ttacccgtat ccggtccaaa aatggttgcg attaacgcaa tcgccatccc ggcgataccg
    32581 aagttgttac cctggcgaga cgtttcatgt ttcgaaagac cggccagact gaagataaac
    32641 aggatcgcgg caacaatgta tgcagctgta actaatcctc cagacatatg ttacccctta
    32701 atttttgcgg aacattttca gcatgcgctg agtcacggtg aagccaccga aaatattaat
    32761 gctggctata agcaccgcga taaaactaag gaagctaacc cagccgccct ggccaatctg
    32821 caacagtgct ccgacaacaa taatccctga aatcgcgttg gtgaccgaca tcaacggtgt
    32881 atgcagcgca tgcgatacat tccacaccac gtaataaccg acaacgcagg ccagcgcgaa
    32941 aacggtgaag tgcccaagaa actctttcgg cgcaacgctt gccagccaac cgaaaaggat
    33001 gattgccagc gccatcaatg cgtatttacg ccacggtgag caggcacatt tttcctcagt
    33061 tttcacttcc ggtgccgctt tttgtgccgc ctgcggctga gctgatacct gaatcagcgg
    33121 tgccggccag gtaatttcgc ccgcacggat cacggtcacg ccgcgaatca ccacatcatc
    33181 aaaatcaaca gtgatattgc cgtctttctc tttgcacaac agtttcagca gattaacgag
    33241 gttagtaccg taaagctgtg aggattgcgt cggcagacgg cccggaagat cggtataacc
    33301 aatcactttg acaccatttt ccgtagtgaa gatttcaccc ggcacggtgt attcacagtt
    33361 gccaccgttt tgggctgcca ggtcgacaat cacactgccc gccttcatgg agtcaaccat
    33421 ttcacgggta attagcttcg gcgctggttt gcctggaata agcgcggtgg tgacaatgat
    33481 atcgacctct tttgcctggg cggcaaagag ttccatttcc gctttgatga acgcgtccga
    33541 catcactttg gcatagccat cgccgctgcc cgcttcctct ttaaaatcca gctcgaggaa
    33601 ttccgcgccc atactttgaa cttgttcttt cacttccggg cgggtgtcga atgcacgcac
    33661 aatcgcgccg agactgtttg ctgcgccaat ggcggccaga cctgcaacac ccgcaccaat
    33721 caccatcact tttgccggtg gcactttccc ggccgcagta atttgcccgg taaagaagcg
    33781 cccaaattca tgtgccgctt caacaatggc gcgataaccg gcgatgttcg ccatcgagct
    33841 tagtgcgtcc agcgattgtg cgcgtgagat acgcggcaca gaatccatcg ccatcacggt
    33901 cacgttacgt tccgcaagtt tttgcattaa ttccggattc tgcgcaggcc agataaaact
    33961 caccagcgtt gtcccaggat tcagtaacgc aatttcatca tctaacggcg cattgacctt
    34021 cagaatgatc tctgactgcc agacgctatt cccttctaca atttcagcgc ccgcttgcac
    34081 aaacgcttta tcgtcaaaac ttgccagttg acccgcgccg ctctctaccg cgacggtaaa
    34141 acccagtttc agcagctgtt ccactgtttt tggcgttgct gcaacacggg tttcattggt
    34201 taaccgttct cttggtatgc caattcgcat gatattccct tccatcggtt ttattgatga
    34261 tggtttgcct gtgtcaggag ccacacaagc cgctcatgta cgagctaaat gttactccgt
    34321 taaaataaat tagtaacaaa cgccctataa cgtactgaaa attatgcctg tgatctagcg
    34381 ccaaaaatca gtatttcggc gtgaactcgc aaaatattaa cgattcagcc gtgatagtgg
    34441 gataaacacc ttagaacgcc ggataaagac tgataattgt cttcgacggt cgggtaaaac
    34501 gagacatcgc cccggcacga atcactactt aacattaaat taacttatat aattcagttg
    34561 cttcagtagt aatgatgctg atacggctgt tttttaagca tagacggtca tttgagcagg
    34621 attaaaattg gcttaaagaa tgtgatatga aaaatgacgc agacagttac accgtttaaa
    34681 tgcaataatc agccacgttt ctcgttaata acaataccag tacctggttt gcgcaaggcg
    34741 aaggattatt tttatgaagc ttaagaacac cctcctggcg tcggcactgc tttctgctac
    34801 ggcattctcc gttaacgcag caacagaact gacaccggag caagcggcag cggttaaacc
    34861 ttttgaccgt gtagtggtta ccggtcgttt taatgctatt ggcgaagcgg tgaaagccgt
    34921 ttctcgtcgc gcagataaag aaggtgccgc ctctttttat gttgtcgaca cttctgattt
    34981 tggtaacagc ggtaactggc gtgtggtcgc tgacctctat aaagccgatg ctgaaaaagc
    35041 agaagaaaca agtaatcgcg taattaacgg tgttgtcgaa ctgccgaaag atcaggctgt
    35101 tctgattgaa ccgtttgaca cggtcaccgt ccagggcttc tatcgtagcc agccagaagt
    35161 caatgatgcc atcaccaaag cggcaaaagc gaaaggtgcc tactctttct acatcgttcg
    35221 tcaaatcgat gccaaccagg gcggcaacca gcgtattact gcattcatct ataaaaaaga
    35281 tgctaagaaa cgtatcgtcc agagcccgga tgtgatcccg gcagattccg aagcaggacg
    35341 tgcagctctg gctgccggtg gcgaagccgc gaagaaagtt gagatcccag gtgttgcgac
    35401 taccgcatca ccaagttctg aagtcggtcg tttctttgaa acccagtcat caaaaggcgg
    35461 gcgttacacc gtcacgctcc cggatggcac taaagtcgaa gaactgaaca aagcgaccgc
    35521 agcgatgatg gtcccgttcg acagcatcaa attctctggc aactacggca acatgaccga
    35581 agtctcctat caggttgcga aacgtgccgc gaagaaaggt gctaagtatt accacatcac
    35641 ccgccagtgg caggaacgtg gtaataacct gaccgtcagc gcagatctgt ataaataact
    35701 attcagttac tcctataagg cggcttgatg ccgcctttcg catttttagt cacaaatttc
    35761 cccgcgacca ttgcatgcag acacatctct tcgtaaaatc ccgcgcttca gtggcaaact
    35821 ccatttttat gcgttttgcc ctaatattta ttcttcttat cacgttttaa tcactggata
    35881 tcgatggaaa agaaactggg actgagcgca ctcaccgcgc tggtattaag ctcaatgctg
    35941 ggcgcgggtg ttttcagtct gccgcaaaat atggcggcag ttgccagccc ggcagcactg
    36001 ctcatcggct ggggtattac tggcgctggc attttattgc tggcctatgc catgctgatc
    36061 ctcacgcgca ttcgtcccga acttgacggc ggtatcttta cctatgcccg cgaagggttt
    36121 ggcgagctaa tcggcttttg ttccgcatgg ggatactggc tgtgcgcagt catcgccaac
    36181 gtctcctatc tggtgatcgt tttttccgcg ttaagctttt ttacggacac gccggaattg
    36241 cgcctgttcg gcgatggtaa tacctggcaa tcgatcgtcg gtgcatcggc tttattgtgg
    36301 attgttcact ttttgatcct gcgcggggtg caaaccgctg ccagcattaa cctggtggcg
    36361 acactggcaa aattgttgcc gctgggtctg tttgttgtgc tggcgatgat gatgttcaaa
    36421 ctggatacct tcaagctcga cttcaccgga cttgcacttg gcgtacccgt ttgggaacag
    36481 gtgaaaaaca ccatgctgat caccctgtgg gttttcattg gtgtggaagg tgccgtggtt
    36541 gtttctgcgc gtgcgcgtaa taaacgtgat gttggcaaag cgacactgct ggcggttctc
    36601 tccgctctgg gcgtttactt gctggtaacg ctgctttcgc tgggtgtggt ggcacgtcct
    36661 gaactggctg aaattcgtaa cccgtcaatg gccggtctga tggtggaaat gatgggacca
    36721 tggggcgaaa tcatcatcgc tgccggtttg atcgtttccg tttgcggcgc gtacttaagc
    36781 tggaccatca tggcggcaga agttccgttc ctggctgcca ctcataaagc attcccacgc
    36841 atttttgcgc gccagaacgc acaagctgcg ccatccgcct cactgtggtt gaccaacatc
    36901 tgcgtgcaaa tttgtctggt actcatctgg ctgaccggtt ccgattacaa cacgttgctg
    36961 accatcgcct cagaaatgat tctggtgccc tatttcctgg tcggcgcgtt cttgctgaaa
    37021 atcgcaaccc gtccactgca taaagcggta ggtgtcggtg cctgcattta tggcttatgg
    37081 ttattgtacg cctctggtcc aatgcacttg ctgttatccg ttgttctgta tgcgccgggg
    37141 ctgctggttt tcctgtatgc gcgcaaaacg catacacatg ataatgtact gaaccgtcag
    37201 gaaatggtgc tgattggcat gctgttaatt gcctcagttc cggcgacctg gatgctggtg
    37261 ggataacgtt catcccatcg ttggatagca aaggagataa cgatgggtaa agcccagccc
    37321 ttgccaatat taattactgg cggaggtcgt cgcatcggcc tcgccctcgc ctggcatttc
    37381 attaatcaaa agcaaccggt gattgtcagc tatcggacgc actatccagc cattgatgga
    37441 ctgattaagg caggtgcgca gtgtattcag gctgattttt cgaccaacga cggtgtgatg
    37501 gcgtttgccg atgaagtact aaaaagcacc catggtctgc gtgctatttt gcataacgcc
    37561 agtgcgtgga tggcagaaaa accaggtaca ccactggccg acgtactggc ttgcatgatg
    37621 cagatccacg ttaatacccc atacctgctc aaccatgcgc tggaaagatt actgcgtggg
    37681 cacggacacg ccgccagcga tatcattcac tttaccgatt atgtggtgga gcgcggtagc
    37741 gacaaacatg ttgcgtatgc tgcaagcaaa gcggcactgg ataatatgac ccgctcgttt
    37801 gcccgcaagc tggcaccgga agtgaaagtg aattctattg cgccatcgct gatcctgttt
    37861 aatgaacatg atgatgccga atatcgacaa caggcgctga ataaatcact gatgaaaacc
    37921 gcgcctggcg agaaagaagt gatcgacctg gtcgattact tacttaccag ttgctttgtc
    37981 accggacgca gtttcccact tgatggcggt cgtcatctgc gttaatgcag ctttatccag
    38041 catataatca acacccatgc gcttatcccc caacacgcaa cagaaccaac aaaagcggcg
    38101 ggcaagcgca tcattccggt gaaataccac agcgacacca ggtaaacaaa atagggaata
    38161 atcgaccaca tactaaaaat gatggttgcg cgtaaggctt caatgccgcg ttcgctggca
    38221 acaatataat gcgcgataag cgcaaaggtc gggaaaagtg gaatcagccc ggcgatataa
    38281 taatttttcg tttttgctaa aacaccaatc aacagcacta ccagcgcacc gagcgcggct
    38341 ttgattacca gccccatttt tttaccttaa cacttccata acaagtcatc agtagaatac
    38401 ctgatgaaaa cttgtttaga aacgattgat agtaagtaaa aacagcgcgg tgtattgtga
    38461 cgtttttata tctaccgtga atgttatgaa cactatcgta tttgtggaag atgatgcgga
    38521 agtcggttca ctgattgccg cgtacctggc aaaacatgat atgcaggtta ccgtagagcc
    38581 gcgcggcgac caggccgaag aaaccatttt gcgagaaaat ccggatttgg tgttactcga
    38641 catcatgcta ccaggcaagg acggcatgac catttgtcgt gatttacgcg caaagtggtc
    38701 tggaccgatt gttcttctaa cctctctcga tagcgatatg aaccacatcc tggcactgga
    38761 aatgggtgcc tgcgactata ttctcaaaac gacgccccct gctgttttgc tagcgcgttt
    38821 acgtttgcat ttgcgtcaga atgagcaagc cacactgagc aaaggtcttc aggaaacgtc
    38881 tctgactccc tacaaagccc tgcatttcgg cacgttgacc atcgatccca tcaaccgcgt
    38941 agtcaccctg gctaacactg aaatctcgct ctcgacagct gatttcgaat tattgtggga
    39001 attagctacc catgccgggc aaatcatgga ccgcgatgca ttgctgaaaa atttacgcgg
    39061 cgtcagttat gacggactgg atcgtagcgt ggacgtggct atttcgcggt taagaaaaaa
    39121 actgctcgat aacgccgcag aaccttatcg cattaaaact gtgcgtaaca aaggctatct
    39181 ttttgcgcct catgcatggg aataagcgat gaaaaaactg tttatccagt tttacctgtt
    39241 attgtttgtc tgcttccttg tgatgtctct gctggttggg ttggtgtaca aatttaccgc
    39301 cgaacgcgcg ggcaaacagt cgctggatga tttgatgaac agttcgctgt atctgatgcg
    39361 cagcgaattg cgtgagatcc ccccacacga ctggggtaag acgctgaaag agatggattt
    39421 aaatctctct ttcgatctgc gtgtcgagcc attgagtaaa taccatcttg atgatatttc
    39481 catgcaccga ctgcgtggcg gcgaaattgt cgccctggac gatcagtaca cgtttttgca
    39541 gcgtatcccg cgcagccact acgtgctggc agttggtcct gttccttatc tttattatct
    39601 ccatcagatg cgattgctgg atatcgccct gatcgctttt attgctattt ccctcgcctt
    39661 tccggtgttt atctggatgc gtccgcactg gcaggatatg ttaaaactgg aagcagcggc
    39721 gcaacgattt ggcgatgggc atctcaatga gcgtatccac tttgatgagg gttcgagctt
    39781 tgaacgactt ggtatcgcat ttaaccagat ggcggacaat atcaacgcct tgattgccag
    39841 caaaaaacag cttattgacg gtatcgctca cgaactgcga acaccgttag tgcgtctgcg
    39901 ttatcgactg gagatgagcg ataacctgag cgccgccgaa tcccaggcgt tgaatcgtga
    39961 tatcagtcaa cttgaagctt taattgaaga gctgctgact tatgcccgac tcgatcgccc
    40021 acaaaacgag cttcatctta gcgaaccaga cctgccgttg tggctgtcaa cgcatctggc
    40081 agacattcag gcagtaacgc ccgataaaac ggtacggatt aaaacgctcg cgcaaggcca
    40141 ttatgcggcg ttggatatgc gcttaatgga gcgcgtgctg gataatttgc tcaataacgc
    40201 cctgcggtac tgccattcaa cggttgaaac cagcctgcta ctgtcgggga atagagcgac
    40261 attaattgtt gaggatgatg gcccagggat tgccccagaa aaccgcgaac atatctttga
    40321 accttttgtt cgcctcgatc ccagccggga tcgctcaacc ggcggctgcg ggctggggct
    40381 ggcaattgtc cactctatag cactggcaat gggcggtacg gttaattgtg acaccagcga
    40441 actgggtggt gctcgcttct cgtttagctg gccgttatgg cataacatcc cgcaatttac
    40501 ctctgcctga cactacgcgc acgatggtca agtcaccacg actgtgctat aaaataagta
    40561 tgttgtaact aaagtggtta atattatggc gcgttacgat ctcgtagacc gactcaacac
    40621 tacctttcgc cagatggaac aagagctggc tgcatttgcc gctcatcttg agcaacacaa
    40681 gctattggtt gcccgcgtgt tctctttgcc ggaggtaaaa aaagaggatg agcataatcc
    40741 gcttaatcgt attgaggtaa aacaacatct cggcaacgac gcgcagtcgc tggcgttgcg
    40801 tcatttccgc catttattta ttcaacaaca gtccgaaaat cgcagcagca aggccgctgt
    40861 ccgtctgcct ggcgtgttgt gttaccaggt cgataacctt tcgcaagcag cgttggtcag
    40921 tcatattcag cacatcaata aactcaagac cacgttcgag catatcgtca cggttgaatc
    40981 agaactcccc accgcggcac gttttgaatg ggtgcatcgt catttgccgg ggctgatcac
    41041 ccttaatgct taccgcacgc tcaccgttct gcacgacccc gccactttac gctttggttg
    41101 ggctaataaa catatcatta agaatttgca tcgtgatgaa gtcctggcac agctggaaaa
    41161 aagcctgaaa tcaccacgca gtgtcgcacc gtggacgcgc gaggagtggc aaagaaaact
    41221 ggagcgagag tatcaggata tcgctgccct gccacagaac gcgaagttaa aaatcaaacg
    41281 tccggtgaag gtgcagccga ttgcccgcgt ctggtacaaa ggagatcaaa aacaagtcca
    41341 acacgcctgc cctacaccgc tgattgcact gattaatcgg gataatggcg cgcgcgtgcc
    41401 ggacgttggt gagttgttaa attacgatgc cgacaatgtg cagcaccgtt ataaaccaca
    41461 ggcgcagccg cttcgtttga tcattccacg gctgcacctg tatgttgcgg attaacgccc
    41521 ggctttcata ctgccgacca tctgttctgg ccgtacccag ctatcaaact cggcttcgct
    41581 aagatacccc agcgcaaggg ccgcagcttt taaggtcagc ccttctttat gcgctttttt
    41641 ggcgatctcc gcggctttgt cataaccaat gtgggtgtta agcgcagtca ccagcatcag
    41701 cgattcattg agtaattgat tgattcgctc acgattcggt tcaataccca ctgcgcagtg
    41761 tttgttaaaa ctttccatgc catctgccag caagcgcacc gattgcagga aattgtggat
    41821 caccattgga cggaagacgt tcagttcaaa gttaccggaa gcgcccccca tgttgatcgc
    41881 cacgtcgttc cccatcacct gacagcagag catggttaat gcctcacact gtgttggatt
    41941 cactttcccc ggcatgattg agctgcccgg ctcattttcc gggattgaga tttcaccaat
    42001 tccgcagcgc gggccagagg ccagccagcg gacatcattg gcgattttca tcagtgacgc
    42061 agccaaccct ttcaacgcgc cgtgcgcctg aaccagggca tcacaggtcg caagcgcttc
    42121 aaatttgttc ggcgcggtaa caaacggtgc acaggtaatg actgccagtt catctgctac
    42181 gcgacgcgca tactccggat gggtatttag tccagtaccc accgctgtac cgccaagagc
    42241 cagttccgct acgtgaggca ggctgtattc gatatgtttg agattatgct cgagcatcgc
    42301 tacccagccg gaaatctcct gccctagtgt tagcggcgtg gcgtcctgca agtgggttcg
    42361 accgattttg acgatatcgg caaatgcacg cgatttttca ctcagcgtct gtgtcagggt
    42421 tttaagctgc ggaatgagtt gcttgcgcag cgccagcagc gccgcaacgt gcatcgccgt
    42481 cggaaagaca tcgttggaac tttggctttt gttcacgtcg tcgttagggt gaactttacg
    42541 ttccatcccg cgcacgccgc cgagtaattc actggcccgg ttagccagca cttcgttcat
    42601 gttcatgtta ctttgcgtgc cggagccggt ctgccagata gccagcggga attcgtcgtc
    42661 atgctgtcct gccagtactt catccgccgc ctgccgaatg gcgctcgctt tctcttcaga
    42721 caacaagcct aaatcttcat taacttttga cgctgcgcgc ttggttagcg ccagcgcatg
    42781 aatcagtgag gtgggcattt tctccgtcga aatgcggaaa tgctccagcg agcgttgagt
    42841 ttgtgcgccc cacagcttat ctgccgggac atcaatcgcc cccatcgaat ctttttcgct
    42901 gcgtactgta ttcatggcct gctcctcacc tgtttaattt tttctttctg ttttgctttc
    42961 gttaagcaac ttttagctca cttattattt actatttgat aacaaatgtt tggtctttcg
    43021 tgccatgtaa aaaaaccgcc ccgaagggcg gctctgttta tttcacacag cgagtgcact
    43081 gtgtgagttg tatctgctgg aagaagtcat ttcctttatc atccacaagg ataaacgccg
    43141 ggaaatcttc cacttcaatt ttccagatgg cttccattcc cagttccgga tattcaacac
    43201 attccaggct cttaatactt ccctgcgcca atacagcggc cggaccaccg atactgccaa
    43261 ggtagaagcc gccgtgtttt ttacaggcat ccgtcacctg ctggctgcgg ttgcctttcg
    43321 ccagcatgat catacttccg ccctgcgctt gcagttgatc gacataagaa tccatccgtc
    43381 cggcggtcgt tgggccaaga gaaccggagg cataaccttc cggcgttttg gccggacccg
    43441 cgtagtaaat cggatgatct ttaatgtact gcggcagccc ttcaccgtta tccatccgct
    43501 ctttcagttt ggcgtgagca atatcacgac cgacgataat cgtgccgtta agcgataagc
    43561 gtgtagaaac gggatactgc gacaactgtg cgaggatctc tttcatcgga cggttaaggt
    43621 caacgcgcac cgcttcgcct tctcccgctt tacgcagctc ttcagggata tatttacctg
    43681 gattatgttc cagtttttcg atccagatcc cctgacggtt gatcttcgct ttgatattac
    43741 ggtcagcaga gcaggaaaca cccataccaa ccggacagga tgcgccgtga cgtggcaggc
    43801 gaatcacgcg tatgtcgtga gcgaagtatt taccaccaaa ctgcgcaccc agaccgagat
    43861 tttgcgcttc gatcagcaat tctttttcca gttccacatc gcggaatgcc tgaccgtgct
    43921 cattcccttc cgttggcagt tcgtcatagt atttagcgga agccagtttc actgttttaa
    43981 ggttagtttc tgcagaagtt ccaccaatta cgaacgcaat atgatacgga ggacaggccg
    44041 ccgtacccag cgtgcgcatc ttctcaacca ggtaattttt cagtttcccc ggcgttagta
    44101 acgctttggt ttcctgatag agatacgtct tgtttgccga accaccacct ttggcgatac
    44161 agaggaattt gtactcgtcg ccatcaacgg cataaagatc gatctgcgct ggcagattgg
    44221 tgccggtatt cacctcttta tacatatcca gcggcgcgtt ttgcgagtag cgcaaattat
    44281 cttcgatata agtgttatag acaccgcgcg ccagcgccgc ttcatcacag ccaccggtcc
    44341 atacacgctg ccctttttta ccaacaataa tcgcagtgcc ggtatcctga caggttggca
    44401 gaacaccttt cgccgcgatg tcggagttgc gcaggaattg cagcgccaca tatttatcat
    44461 tttcgctggc ttccgggtca cgcagaatgt cggccacctg ttgttggtga gccggacgca
    44521 gcataaatga cgcatcatga aatgcctgac gcgccaacag agttaacgct tcgggtgcga
    44581 ctttcaaaat ctcctgccct tcaaattcag atacgctaac gtgttcgctg gttagcaggt
    44641 aatactcagt atcatctttt ttgagtggaa aaggagcctg ataatgaaag ggtttgtttg
    44701 acattgttct ctcacttact gcctggtttg gttatgctct gggcgggtgt tccgttgccc
    44761 tgttaaaagc gagtaacaat atcctacaca cttttttaac aaaaactgag actagtacga
    44821 ctttttgcgg ctccaggtta cttcccgtag gattcttgct ttaatagcgg gattaatttc
    44881 cacattaaaa cagggattga tcatgcaaaa actcattaac tcagtgcaaa actatgcctg
    44941 gggcagcaaa acggcgttga ctgaacttta tggtatggaa aatccgtcca gccagccgat
    45001 ggccgagctg tggatgggcg cacatccgaa aagcagttca cgggtgcaga atgccgccgg
    45061 agatatcgtt tcactgcgtg atgtgattga gagtaataaa tcgactctgc tcggagaggc
    45121 cgttgccaaa cgctttggcg aactgccttt cctgttcaaa gtattatgcg cagcacagcc
    45181 gctctccatt caggttcatc caaacaaacg caattctgaa atcggttttg ccaaagaaaa
    45241 tgccgcaggt atcccgatgg atgccgccga gcgtaactat aaagatccta accacaagcc
    45301 ggagctggtt tttgcgctga cgcctttcct tgcgatgaac gcgtttcgtg agttttccga
    45361 gattgtctcc ctactccagc cagtcgcagg tgcacatccg gcaattgctc actttttaca
    45421 acagcctaat gccgaacgtt taagcgaact gttcgccagc ctgttgaata tgcagggtga
    45481 agaaaaatcc cgcgcgctgg cgattttaaa atcggcgctc gacagtcagc agggtgaacc
    45541 gtggcaaacg attcgtttaa tttctgaatt ttacccggaa gacagcggcc tgttctcccc
    45601 gctgttactg aatgtggtga aattgaatcc tggcgaagcg atgttcctgt tcgctgaaac
    45661 accgcacgct tacctgcaag gcgtggcgct ggaagtgatg gcgaactccg ataacgtgct
    45721 gcgtgcgggt ctgacgccta agtacattga tattccggaa ctggttgcca atgtgaaatt
    45781 cgaagccaaa ccggctaacc agttgttgac ccagccggtg aaacaaggtg cagaactgga
    45841 cttcccgatt ccagtggatg attttgcctt ctcgctgcat gaccttagtg ataaagaaac
    45901 caccattagc cagcagagtg ccgccatttt gttctgcgtc gaaggcgatg caacgttgtg
    45961 gaaaggttct cagcagttac agcttaaacc gggtgaatca gcgtttattg ccgccaacga
    46021 atcaccggtg actgtcaaag gccacggccg tttagcgcgt gtttacaaca agctgtaaga
    46081 gcttactgaa aaaattaaca tctcttgcta agcttattaa aggcttataa caccttcagg
    46141 cggccagtcc gcctgatttc attttatgga taatcattat gaataaatcg ctggtagcgg
    46201 taggcgtcat tgttgcgcta ggcgtagtct ggacaggcgg cgcatggtat acaggcaaga
    46261 agattgaaac ccatctcgaa gacatggtcg cgcaggcgaa cgcgcaactc aaactgaccg
    46321 ctcctgaatc caacctggaa gtgagttatc aaaactatca tcgcggcgta ttcagcagtc
    46381 agctgcaact gttggtgaaa cccattgccg ggaaagaaaa tccgtggatt aaaagcggtc
    46441 agagcgtcat cttcaacgaa tcggttgatc atggtccctt cccgcttgcc cagcttaaaa
    46501 aactgaacct gatcccgtcg atggcatcaa ttcaaaccac gctggttaat aacgaagtaa
    46561 gcaaaccact gtttgatatg gcaaaaggtg aaacgccttt tgagattaac tcgcgcattg
    46621 gttacagcgg tgattccagt tccgatattt cgctcaagcc actgaattac gagcaaaagg
    46681 atgaaaaagt cgcctttagc ggcggcgagt tccagttaaa tgcggacaga gacggcaaag
    46741 ctatctccct ttccggggag gcgcaaagtg gtcggataga cgcagttaac gaatacaacc
    46801 agaaagtgca gttgaccttt aataatctga aaaccgacgg ttccagcacg ctggcaagtt
    46861 ttggtgagcg cgtaggaaac caaaaactgt cactggaaaa aatgaccatt tcggtggaag
    46921 gcaaagaact ggcactgctg gaaggcatgg agatcagcgg taaatcggat ctggtcaatg
    46981 atggtaaaac gatcaatagc caactggatt actcgctaaa cagcctgaag gtacagaatc
    47041 aggatctggg cagcggcaag ctgactttaa aagtcggcca gattgatggt gaagcctggc
    47101 atcagtttag ccagcaatat aacgcgcaaa ctcaggcgct gctggcacag ccagaaattg
    47161 ccaacaatcc cgaactttat caggagaaag tgacggaagc cttctttagc gccctgccgc
    47221 tgatgttgaa aggcgatccg gtgattacta tcgcgccgct aagctggaaa aacagtcagg
    47281 gtgaaagtgc gctgaatctg tcgctgttcc tgaaagatcc ggcaacgact aaagaagcgc
    47341 cgcaaacgct ggcgcaggaa gtggaccgct cggttaaatc tctggatgcg aaactgacca
    47401 ttccggtgga tatggcaact gagtttatga ctcaggtagc gaagctggaa ggttatcagg
    47461 aagatcaagc gaaaaaactg gcgaaacagc aagttgaagg tgcatcagca atggggcaga
    47521 tgttccgtct gactaccttg caggacaata ccatcaccac cagcctgcaa tatgctaacg
    47581 gtcagataac gttaaatggg cagaaaatgt cgttagaaga ttttgttggc atgtttgcga
    47641 tgccagctct taacgttccg gctgtaccgg caattccgca gcagtaattc acgaccaaaa
    47701 cgccggagaa ttttcactcc ggcgttttca tctgaaaaca aaccagagat tgcctgatgc
    47761 gctacgctta tcaggcctac ctttacctgc aacactttga atttatgagt ttttgtaggc
    47821 tggataaggc gtttacgccg catccggcat aaaaaacgcg cactttgtca acaatctgaa
    47881 acgccggagg tttttctctc cggcgttatt tcttacttca gcataaagtc atactgaata
    47941 ctcattttca catcccagta attcaggcga tcgcccagcg tactgccatc gcgcttttcc
    48001 cgccctttat cggcagcaag aaagaaattc atcccttgta acggcccttc gctgacccga
    48061 tagttcatac cacctaagat acccatgcct tcaatccgca catctcgttt actggattca
    48121 tagccgcgag tcacaatcgg tgcaaatgtc agtgtaaatt gcggcgttaa acgatagttg
    48181 acgcccaatt gccaggactg cattccttca cggttacgat caagacttaa tgaaaaggga
    48241 tagccaatgt cggtatccac cacggcacca ttcattgcat ggcgcaaata accaccactg
    48301 ccaaatacgc ttccccacgg cgcattccag gtcaattgtc cactaaccaa cgccgtttca
    48361 ttgggctggc tggtattacg acttaaacct tcgagctgcg cgtaaaagcc gagcaactcc
    48421 cctttcaacg tataattcgg gtcgagcggt atgcttttct cgagaatact ttgtatctga
    48481 ttggaaacat ctttctgtac cgatgccccc agagaatagc gactggtatc atcatgccaa
    48541 ctggcggcta aaaaagaacg tgcgcgatgt ttatcgtatt ttcccgtctg gctgttatac
    48601 accccttcat cacgaatacc ggttgttgaa tcagtccata aaaatactcg tgaacgccac
    48661 attccccaca gatcaatatt tggtgttaaa gcccaataac ctaatgctcc ttcagaaact
    48721 gtcggtaatg tacgttgcga actgccagcc attaacggaa tacgataagg taagctacca
    48781 taactataat ccgtcccgaa gcgacctaat ttaagcaggc tattttcact gggttttatt
    48841 tttactgccc ccagggcaaa gccaaaactt ttatcaccat caagatacca ccgggtactc
    48901 atatcagcac gagcacctaa tttataaaca taatacgccc cgccttcaac gccaataata
    48961 ttggaataat aaccactatt gaaatcgagc aatcctccct gtacccatgc gtaaatatct
    49021 cttcccgcgc cagcactggg cttatccgcc ctgcgcaatt cgtttttcag tctgatgcgt
    49081 agtcccgctt catcgtcagc cagttgcgcc gcataagcag aggtaaggcc agaggcagcc
    49141 gtcaggcaaa taacggccat ggccactatt tttctcatag ttgcaccttt gatgttctga
    49201 ttatttttat tgaatattaa ttagtgatat cgctgattaa ttgctgctgc acttttttac
    49261 gattatcaat ttcaaccacg atttctttga attttttatc cgtgagcgga taaaaccaga
    49321 taataacgaa tgccagtagc ataaatccgc aaggtactaa ggcaattgat gtgcggatgc
    49381 ccataataac ttccggcgtt tgcacctgat tggcgatata tccgcttaat cccaaaataa
    49441 aggcaggaat tgaacctccg attgcctgac cgcatttacg ggtaaatgag aatagtgaat
    49501 aggtgagccc ttcaattcgc acgccggtca ggtattcacc gtattctacg gtatcagctt
    49561 ccagtgccca catcacagtc atagtaacgc cctgaccaat tgaagcgatg gccaacgcaa
    49621 ccaacgccac cggcagtgac cagacggaaa cccagaagaa tagcagataa ccgcaggttc
    49681 ccagcaaagc gccaatcagg aaggtattct ttttaccgat cctcgcgacc atccccggca
    49741 ccagcggtgc cgatgccaca gtaccaacca ggttttgcac cagtaccagc acagtgaaca
    49801 gcccggtatc atttaacaca tagcgcacgt agaacaacga cgaggcgctg accgcgaagg
    49861 tcgaaatcag cacacacagc gcaccgatgc acaacataaa cagcgggcga ttccgtttca
    49921 gggtttgcag actgatcttc aatgacggct gcgccacgat acgtaccaca ttctcacgcg
    49981 tcgatttgaa gcagatgaag taaagcacca ttccggcaat cgccagcaca atcgtccaga
    50041 aatggtatac cgacaccatc tcttccgggc tggagttctt aatgctcggt cctatcagaa
    50101 atgccaggca gacaaaggtt aatgaagcgg caatcccgcg agccgcgccc agacgggcgc
    50161 gggattgtgg ttgttgggtc atcgcggtag caagtgaacc ataaggaata ttcaccaggc
    50221 tgtagcaaag cccgaggccc atgtaggtca aatatgcata cactactttg ctgctatggc
    50281 tccagtcggt cggtacccag aataccagca cgctgaagat cattaacggc gcagtaccga
    50341 agagtaaaaa cgggcggaat tttccccagc gggtattcac actgtccacc actcgtccgg
    50401 caaagacgtc ggcgaaggca tcgaataccc gcaccagtaa cagcatggtg cccgccgcag
    50461 cggcaccgac gccagcgacg tcggtgtagt aactcaatag gaagagcgcc cccattgcga
    50521 aggcgaagtt attggcgacg tcaccgaggc tgtagccgac gatggtgcgc caggagagtt
    50581 gttgattcat tgtttgcctc cctgctgcgg tttttcaccg aagttcatgc cagtccagcg
    50641 tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg cgagtgaaga tccctttctt
    50701 gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa tcggcgaaat tccatacctg
    50761 ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga tacatatcca gccatgcaca
    50821 ctgatactct tcactccaca tgtcggtgta cattgagtgc agcccggcta acgtatccac
    50881 gccgtattcg gtgatgataa tcggctgatg cagtttctcc tgccaggcca gaagttcttt
    50941 ttccagtacc ttctctgccg tttccaaatc gccgctttgg acataccatc cgtaataacg
    51001 gttcaggcac agcacatcaa agagatcgct gatggtatcg gtgtgagcgt cgcagaacat
    51061 tacattgacg caggtgatcg gacgcgtcgg gtcgagttta cgcgttgctt ccgccagtgg
    51121 cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt tcgttggcaa tactccacat
    51181 caccacgctt gggtggtttt tgtcacgcgc tatcagctct ttaatcgcct gtaagtgcgc
    51241 ttgctgagtt tccccgttga ctgcctcttc gctgtacagt tctttcggct tgttgcccgc
    51301 ttcgaaacca atgcctaaag agaggttaaa gccgacagca gcagtttcat caatcaccac
    51361 gatgccatgt tcatctgccc agtcgagcat ctcttcagcg taagggtaat gcgaggtacg
    51421 gtaggagttg gccccaatcc agtccattaa tgcgtggtcg tgcaccatca gcacgttatc
    51481 gaatcctttg ccacgcaagt ccgcatcttc atgacgacca aagccagtaa agtagaacgg
    51541 tttgtggtta atcaggaact gttcgccctt cactgccact gaccggatgc cgacgcgaag
    51601 cgggtagata tcacactctg tctggctttt ggctgtgacg cacagttcat agagataacc
    51661 ttcacccggt tgccagaggt gcggattcac cacttgcaaa gtcccgctag tgccttgtcc
    51721 agttgcaacc acctgttgat ccgcatcacg cagttcaacg ctgacatcac cattggccac
    51781 cacctgccag tcaacagacg cgtggttaca gtcttgcgca acatgcgtca ccacggtgat
    51841 atcgtccacc caggtgttcg gcgtggtgta gagcattacg ctgcgatgga tcccggcata
    51901 gttaaagaaa tcatggaagt aagactgctt tttcttgccg ttttcgtcgg taatcaccat
    51961 tcccggcggg atagtctgcc agttcagttc gttgttcaca caaacggtga tacgtacact
    52021 tttcccggca ataacatacg gcgtgacatc ggcttcaaat ggcgtatagc cgccctgatg
    52081 ctccatcact tcctgattat tgacccacac tttgccgtaa tgagtgaccg catcgaaacg
    52141 cagcacgata cgctggcctg cccaaccttt cggtataaag acttcgcgct gataccagac
    52201 gttgcccgca taattacgaa tatctgcatc ggcgaactga tcgttaaaac tgcctggcac
    52261 agcaattgcc cggctttctt gtaacgcgct ttcccaccaa cgctgatcaa ttccacagtt
    52321 ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt ttgatttcac gggttggggt
    52381 ttctacagga cgtaacataa gggactcctc attaagataa aaatactggt caacctttaa
    52441 tctgattagt taaccaattc tgtgttgcac acagttatct gtgagaaacc catggaagag
    52501 aaatggaaat aaaaagaact gacaccgcga tcaaaaaacc aaaaactgca atacccacag
    52561 cgtgatattg atcgcattaa tctttaaaac ggcgttgaca gaagccataa aataagcaac
    52621 cgaacgttcg ttcgagtata ttgggaacga taaactctca tgacatatgg ctacggtgaa
    52681 tatttttggg ggggaattct ccgatcccgg ctagattgta ttttgtacag ggaaattaaa
    52741 gtatgaaacg cgggcacagt tatcaccagc ccgcgaaatg agaggatcag gatgcggtta
    52801 agataccgcc aatcatcgcg cgtaatccct gagcaaacga cgcttcacgc gccagatctt
    52861 ccgcttcgat atcggccagt acaccgtagg tcagatccag cagtaaacgt gaagtcattg
    52921 acgtgttgat gtctgggctt atctcgcctt tttgctgggc atcacgcatc cgctgggcaa
    52981 caaattcagt gatcgtcata tgcttatttt taagcatggc ggcgacctgt gggttacgca
    53041 tcccttccgc cataatttcg accaccagcg cccgttgccc aaaggcttca tgggtgagag
    53101 agacaataga ctcgaccata tagtcaacaa aatgaatccc ttcaatcggt tcccggaaac
    53161 gggccagcgc cctctcctgg tcctgtaaga taatcgcctg aatcaaggct tctttggaga
    53221 tgaaatggtg atagagcgtc ccgggactaa tggcgcagga tttacagatg gctttcatcg
    53281 aggcactgtg aaatccattt tctgaaaaaa tctctctggc agcattgagg atccgggtcc
    53341 gtgtcggttg tgcttcagtc tgcatgttat ccatcattaa accattatag ttagcgtgaa
    53401 taactagttt accgcgttgt caggtgagcg cctatcattg ctgttgaata gtgcgaaggc
    53461 acactctatt cgtatgcttt tcatccagct attggttaac caatagccgt gatttggctg
    53521 ccagttgttt agcagagacg ttgatgaatt gcacacgcta tcccggtaaa caacacatac
    53581 ggctgtttac cggttcgtta gtgtattaat tgagctcctg taccccacca ccggagacgg
    53641 tgagaatttg tccgcttacc cagctggctg caggcgagca aaggaacagc gccgcgttag
    53701 caatatcttg cggttggccc agacgtctga tcggcgtgtg ctgtaacatt ttttgttcaa
    53761 tttctggtgt aataacggat ttcagggcat cggttaatat tgcccccggc gcaatgccat
    53821 tcacccgaat atttttttca cccaggtcaa atgccatatt tctgaccaga tgactggccg
    53881 cagctttaga tgatgcatag gaagtcatgt ttatattttt attttctgcc gccatagaag
    53941 tgatggtcag aataacgcca ccgccatttt tttccatttc tggcgcaaca agttgtgaca
    54001 gatggaaaaa agaaaacaca ttcagttcat aagcacggcg aaaatctgcc attggcatat
    54061 caaacggttt aggaccaccg ccaccggcgt tgttaaccag gatatcaact ttacccagct
    54121 tactgacggc aaagtctgcc agtgcagaga gttcctgttc ggaagtaata tcacaacggc
    54181 aggcaaatgc ctgaccaccc agttgttgaa tttcgtctac aacatggtta gctgcgtcgg
    54241 cgttaatatc actgaccacc acagatgcgc cagctgtcgc gaatgtaatg gcgatttctt
    54301 taccaatacc tgcacccgca cctgtgatga tggcgcattt tccgtcgagt ctcaggttgt
    54361 cagaattaaa cacataaacc tcctgtgaac ttcagtaaaa ataaaaggca ccgcctgtgt
    54421 gccacatttt ttatcattaa ccttgaggaa aggacactac caggtatagc agataaagag
    54481 aggattattt cgccgcaatg agccgggcgg gaataataag attgcgtgaa tgcgtctctt
    54541 catgggtgat tttttgcatc atgcgatccg caagtgtggt accaagttcc cgcgcaggcg
    54601 tgctggccca ggtaacgggt atatcatcaa gtgtggttgg tgtcgcatcg gtaaatgccg
    54661 ccagcgaaac ttgttgctca aagtaacgat cgactccgct ttccccgctt tgccgccctg
    54721 ctttgagcaa accaaaccat gcccccatcg caatagtttc gttatagcaa accacagcac
    54781 tgatggtcgg gttatgacgt aaaagcgccg tgatagcttc cgcggcttgc ttctggctgg
    54841 atgtgcactc cagcacccaa tcgctgtgaa acggcaggcc aaattttagt agagttgcac
    54901 aatagccccc cacccgttct gcacgggtta atgaggaact ttgccctccc agccaggcga
    54961 tccgctgatg cccattgcga atgagatgct ccgtcaacaa ctgtgcagcc tgcatgttgt
    55021 ccgggcgaac cgtatcaaca tcatcaagat aactggcacg ggaagcgaaa atcaccggga
    55081 tagctttttc ttctgccatc cgtcgcaggt catcgctgct tcctgcagcc ccggcaatta
    55141 ccacaccatc cacaccctga ttcagtaaca gtgaaaaccg ctgtgccagc tgctcgccgt
    55201 ctttaccgcc gtgaagcaaa aaaaccatcc gtccctgcgc ttccagagct tccgtcaatc
    55261 cggccgtcaa ttcggcgtaa aacggcgcag ataaatcacg gacgatcaaa ccaatgacgc
    55321 cgctttgccc gccgcgcagc gccgacgcct ggcgattgcg cacaaatccc agctcttcaa
    55381 tggcggcgtt aacgcgttct cctgtggcgg tagagattcg ccctttgcca ctaagcacca
    55441 gcgaaacggt acttaccgac acgcccgcag ccagcgcaac atcatgaatg gttatttttt
    55501 tggcggtagc catgcagatg acctactccc tgattatgtg acagataaaa cgttttacct
    55561 tttatttatc ttatacccgc tattatcgtt gcgtaatgtg atttatgcct cactaaaatt
    55621 tgataaaacg ttttatcttc tcgcgcaatt tactgaatcc agattgttct ctacgaggag
    55681 tcgttttatg acggcgaaaa cagcaccgaa agtcacgctg tgggagttct tccagcagtt
    55741 aggcaaaacc ttcatgttac ccgtggcatt attgtcgttc tgcggcatta tgctcggcat
    55801 tggtagttct cttagcagcc atgatgtcat aaccctgatc ccggtcctgg gcaaccccgt
    55861 gttgcaggct atctttacct ggatgagtaa gattggctcg tttgctttta gtttcctgcc
    55921 tgtcatgttc tgtatcgcca tcccgctggg cctggcacgc gaaaataaag gcgtagcggc
    55981 attcgctggc ttcgtcggtt atgcggtaat gaacctcgcg gtaaacttct ggttgaccaa
    56041 taaaggcatt ctgccaacca cggatgccgc ggttctgaaa gccaataaca tccagagcat
    56101 tcttgggatc cagtcgatcg ataccgggat cctcggtgcg gtgatcgccg gtattatcgt
    56161 ctggatgctg catgagcgtt tccataatat ccgcctgccg gatgcgctgg cattcttcgg
    56221 cggtacgcgc ttcgtaccaa ttatctcctc gctggtgatg ggccttgtcg gcctggtgat
    56281 tccattagtc tggccgattt tcgccatggg tattagcggc ttgggccata tgataaacag
    56341 cgcgggtgat ttcggaccga tgctgtttgg taccggtgaa cgtctgctgt tgccgtttgg
    56401 tctgcatcac attctggtag cattaattcg ctttaccgac gcaggcggca cgcaggaagt
    56461 ctgcggtcaa accgtcagcg gcgcactgac catcttccag gcgcaattga gttgcccgac
    56521 cactcacggt ttttctgaaa gcgccacgcg tttcctttcg caaggtaaaa tgcctgcgtt
    56581 tctcggcggt ctgccgggtg cagcgttagc tatgtatcac tgcgcgcgcc cggaaaatcg
    56641 ccataaaatt aaaggtctgc tgatttctgg cctgatcgcc tgcgtcgttg gcggcactac
    56701 cgaaccgctg gaattcctgt tcctgttcgt agcgccagtt ctgtatgtca tccacgcgct
    56761 gttaaccggc ctcggcttca ccgtcatgtc tgtgctcggc gtcaccatcg gtaataccga
    56821 cggcaatatc atcgacttcg tggtgttcgg tattttgcat ggtctgtcaa ccaagtggta
    56881 catggtgcca gtggtggcgg caatctggtt tgtcgtttac tacgtcatct tccgtttcgc
    56941 tatcacccgc ttcaatctga aaaccccggg gcgcgatagc gaagttgcca gctcaatcga
    57001 aaaagccgtt gccggtgcgc cgggtaaatc aggttacaac gttcctgcaa tcctcgaagc
    57061 attaggcggt gccgacaata ttgtcagcct cgataactgc attacccgtc tgcgtttgtc
    57121 tgtgaaagat atgtcgcttg ttaatgtgca ggcactgaag gacaatcggg caattggcgt
    57181 agtacaactt aatcaacata acctgcaggt tgttatcggg ccacaagttc agtcagtaaa
    57241 agatgaaatg gccggtctga tgcatactgt ccaggcataa ggataagata tgttcgattt
    57301 ttcaaaggtc gtggatcgtc atggcacatg gtgtacacag tgggattatg tcgctgaccg
    57361 tttcggcact gctgacctgt taccgttcac gatttcagac atggattttg ccactgcccc
    57421 ctgcattatc gaggcgctga atcagcgcct gatgcacggc gtatttggct acagccgctg
    57481 gaaaaacgat gagtttctcg cggctattgc ccactggttt tccacccagc attacaccgc
    57541 aattgatacg cagacggtgg tgtatggccc ttctgtcatc tatatggttt cagaactgat
    57601 tcgtcagtgg tctgaaacag gtgaaggcgt ggtgatccac acgcccgcct atgacgcatt
    57661 ttacaaggcc attgaaggta accagcgcac agtaatgccc gttgctttag agaagcaggc
    57721 tgacgactgg ttttgcgata tgggcaagct ggaagccgtg ttggcgaaac cagaatgtaa
    57781 aattatgctc ctgtgtagcc cacagaatcc taccgggaaa gtgtggacgt gcgatgagct
    57841 ggagatcatg gctgacctgt gcgagcgtca tggtgtgcgg gttatttccg atgaaatcca
    57901 tatggatatg gtctggggcg agcagccgca tattccctgg agtaatgtgg cgcggggaga
    57961 ctgggcgttg ctgacgtcgg gctcgaaaag tttcaatatt cccgcgctga ccggtgctta
    58021 cgggattata gaaaatagca gcagccgtga tgcctattta tcggcactga aaggacgtga
    58081 tgggctttct tccccttcgg tactggcgtt aactgcccat atcgccgcct atcagcaagg
    58141 tgcaccgtgg ctggatgcct tacgcgtcta tctgaaagat aacctgacgt atatctcaga
    58201 taaaatgaac gccgcgtttc ctgaactcaa ctggcagatc ccgcaatcca cttatctggc
    58261 ctggcttgat ctacgtccgt tgaatattga cgacaacgcg ttgcaaaaag cgcttatcga
    58321 gcaagaaaaa gtcgcgatca tgccggggta tacctatggt gaagaaggtc gtggttttgt
    58381 ccgtctcaat gccggctgcc cacgttcgaa actggaaaaa ggtgtggctg gattaataaa
    58441 cgccatccgc gctgttcgct aaccccaatt gcgcaacgta aaaaatcttt gcgcaatcgt
    58501 ggatttttac cctgctttgt ttttataatg gtgcgcactt ttatattcag aaaaagagtg
    58561 cgaccatgat tgataccacc ctgccattaa ctgatatcca tcgccacctt gatggcaaca
    58621 ttcgtcccca gaccattctt gaacttggcc gccagtataa tatctcgctt cctgcacaat
    58681 ccctggaaac actgattccc cacgttcagg tcattgccaa cgaacccgat ctggtgagct
    58741 ttctgaccaa acttgactgg ggcgttaaag ttctcgcctc tcttgatgcc tgtcgccgcg
    58801 tggcatttga aaacattgaa gatgcagccc gtcacggcct gcactatgtc gagctgcgtt
    58861 tttcaccagg ctacatggca atggcacatc agctgcctgt agcgggtgtt gtcgaagcgg
    58921 tgatcgatgg cgtacgtgaa ggttgccgca cctttggtgt gcaggcgaag cttatcggca
    58981 ttatgagccg gaccttcggc gaagccgcct gtcagcaaga gctggaggcc tttttagccc
    59041 accgtgacca gattaccgca cttgatttag ccggtgatga acttggtttc ccgggaagtc
    59101 tgttcctttc tcacttcaac cgcgcgcgtg atgcgggctg gcatattacc gtccatgcag
    59161 gcgaagctgc cgggccggaa agcatctggc aggcgattcg tgaactgggt gcggagcgta
    59221 ttggacatgg cgtaaaagcc attgaagatc gggcgctgat ggattttctc gccgagcaac
    59281 aaattggtat tgaatcctgt ctgacctcca atattcagac cagcaccgta gcagagctgg
    59341 ctgcacatcc gctgaaaacg ttccttgagc atggcattcg tgccagcatt aacactgacg
    59401 atcccggcgt acagggagtg gatatcattc acgaatatac cgttgccgcg ccagctgctg
    59461 ggttatcccg cgagcaaatc cgccaggcac agattaatgg tctggaaatg gctttcctca
    59521 gcgctgagga aaaacgcgca ctgcgagaaa aagtcgccgc gaagtaacaa aatggatggt
    59581 gcaaattgca ccatccattt ttcatgcaag gcacaaagtc gcgcgatgtt tggcggattc
    59641 gatgcccagc tcaatcaact ccattacctg gattgcctgg cttgccggaa ccggattttc
    59701 accatcgcca tttaacgcat cacgaatagc cgcatagtaa gccggataat tcccaggcac
    59761 cgtcaacagc gtttcttcga cacgttcctc accttccacg cgggtaagta cgccatcacg
    59821 catatcgtag ccccagtctt cctgcggtag acgctcgcca tttttcagac gttcttcctg
    59881 tggatcgagg ccatatttca cataactgcc tcgggatcca tgcacgatat accgtgctga
    59941 ctcagcggct gccagcatgg taccgtgtaa aatgactcgc cgctgtggat aggacaagat
    60001 ggcgtggaaa taatcggtcg actgcgctcc gggccgtaac tgtgccaaat ctaccgtcat
    60061 gctgaccggt aaaccaaaaa gcgtaatggc ctgatcaaga agatgtggtg ctaaatcgta
    60121 ccagataccg ctgcctgggc cgccctgttc acgccaacga tcgcgcacct gcggacggaa
    60181 gcggtcaaaa tgagactcaa agtaagcaac ttcacccagc acgccttccg cgagtaaacc
    60241 ttttagcgtc aagaaatcgc tatcccagcg acggttatgg aatacagaca gcacacgccc
    60301 caggcttttt gccagcgcat ccagctctcg cgcttgtgac agtgtcacgg taaagggttt
    60361 atcaacgacc acatgtttac ccgcctcaag cgccgctttg gctaacggga aatgggtatc
    60421 gttgggtgta ggaatgacaa tcaggtctat gttgggatcg ttaaacagat gcttcggctc
    60481 agagacaacc gtaaccgttg gccagtcggc ttttactttt gtttcatcac tgctggagat
    60541 taccgccagt tccagcccgg gcgtgcccgc aatcaggggc gcatggaagg ttttgctcgc
    60601 ataaccatac ccaatcaacc caacacggat gttgtcgctc atgtcattgc ctctcattga
    60661 agtatgatgg ctatttgaca ctatccttta cccacgctca acagtttaat aacctgccag
    60721 caataaggga tgttgtttaa cttaagtcaa aaaaatagcg aattttccaa cgacaaaagc
    60781 taaatatcgc aaaaacctca gtaaaaatct tgctggagct attattgcta agtaacattt
    60841 accccctgaa gttaatggat caatcaagag agatgtgggc tgtaatgaat cgtcttattg
    60901 aattaacagg ttggatcgtt cttgtcgttt cagtcattct tcttggcgtg gcgagtcaca
    60961 ttgacaacta tcagccacct gaacagagtg cttcggtaca acacaagtaa gctctgcact
    61021 tgtggagcga catgctgccc gtccgggtgc atgttttcac ttgtcggata ttaaaccagg
    61081 aatttattat cttgttcgat gttgttggtg attgtcaggg atagtaaagc gcgttactct
    61141 tttcagagca gtttttcatc cctgaatatt tgccatcagc gcattcttat tattgtaata
    61201 acacaagcgt tcacttttgg tgaaatttta tcatcactaa taatacctac ccctttggta
    61261 ttaattattt tatggacctt ttatgactgt tcaggactac ttattaaaat ttcgcaaaat
    61321 cagttcactc gaaagtctgg aaaaactcta cgaccatctt aattacaccc tgacggacga
    61381 tcaggaactg atcaatatgt atcgtgctgc cgatcaccgt cgcgcagagc tggtttctgg
    61441 cgggcgtttg tttgacctcg gccaggtacc gaagtccgtc tggcactatg tccaataaag
    61501 aaagtagcga tcggttataa aagccttata ataacgcccc taaaattaga tttgcctgtt
    61561 tgcgtcgcta ttctggagaa cctatgacca ccacaacgcc acaacgtatt ggaggctggt
    61621 tgcttggccc gttggcctgg cttttagtcg ctctgttgag tacgacgctg gcgttgttgc
    61681 tgtataccgc tgcgttatct tctccgcaaa catttcaaac gctcggcgga caagcactga
    61741 cgacacaaat cttatggggc gtttctttta ttaccgctat cgccatgtgg tattacacac
    61801 tgtggttgac catcgctttc tttaaacgtc ggcgttgtgt tcctaaacac tatattatct
    61861 ggctgcttat ttccgtattg ctggcagtaa aagcattcgc tttttcaccc gtggaagatg
    61921 gcatagccgt tcgccagttg ctgtttactt tgttggcaac cgcattgatc gtcccctatt
    61981 tcaaacgctc gtcgcgggta aaagcgacgt ttgtgaatcc gtaataacct tacagttaac
    62041 ctgttgtcgc ctgctctgga ttaacggata ataggcggct tttttatttc aggccgaaaa
    62101 atgactgact acctgttact gtttgtcgga actgtactgg tcaataactt tgtactggtc
    62161 aagtttctcg gtctctgtcc gtttatgggg gtttccaaaa aactggaaac cgcgatgggc
    62221 atggggctgg caacaacgtt tgtgatgacg ctggcgtcaa tttgcgcctg gcttatcgat
    62281 acgtggattt tgatcccact taatctgatt tacctgcgca ccctggcatt tattctggtg
    62341 attgctgtgg tcgtgcagtt caccgagatg gtggtgcgca aaaccagccc ggtgctttac
    62401 cgtctgctgg ggattttttt gccgcttatc accaccaact gtgcagtgct cggcgtggcg
    62461 ttgctgaata tcaatctcgg gcacaatttc ttgcagtcgg cgctgtacgg tttttccgcc
    62521 gctgtcggtt tctcgctggt gatggtgctc ttcgccgcca tccgcgaacg ccttgctgtg
    62581 gctgatgtcc cggcaccttt tcgcggtaat gccattgcgt taattaccgc aggtcttatg
    62641 tctctggcct ttatgggctt tagtggtttg gtgaagttgt aatgaatgct atctggattg
    62701 ccgttgccgc cgtgagcctg ctgggcctgg cgtttggcgc cattctgggt tatgcctccc
    62761 gccgttttgc ggtggaagac gatccggtcg ttgagaaaat tgacgaaatc ttaccgcaga
    62821 gccagtgtgg tcagtgcggt tatcccggct gtcgccccta cgcggaagcc atcagctgta
    62881 acggtgaaaa aatcaaccgt tgcgccccag gtggcgaagc tgtgatgcta aaaattgccg
    62941 agttgcttaa tgtcgagccg cagccgctgg atggcgaagc gcaagagcta acgcctgcgc
    63001 ggatggtggc ggttattgat gaaaataact gtattggctg cactaaatgt attcaggctt
    63061 gtccggtaga cgccatcgtt ggcgctaccc gtgccatgca tacggtaatg agtgatctct
    63121 gtacgggctg caatttatgt gttgatccgt gcccgacgca ctgcatctcg ttgcaaccgg
    63181 tcgcagaaac acctgactcc tggaaatggg atctgaacac cattcccgtg cgtatcattc
    63241 ccgtggaaca ccatgcttaa gttattctct gcattcagaa aaaataaaat ctgggatttc
    63301 aacggcggca tccatccacc ggagatgaaa acccagtcca acggtacacc cctgcgccag
    63361 gtaccactgg cgcagcgttt tgttattcca ctgaaacagc atattggcgc tgaaggtgag
    63421 ttgtgcgtta gcgtcggcga taaagtattg cgcggccagc cgcttacccg tggtcgcggc
    63481 aaaatgctgc ctgttcacgc gcccacctcg ggtaccgtta cggctattgc gccccactct
    63541 acggctcatc cttcagcttt agctgaatta agcgtgatta ttgatgccga tggtgaagac
    63601 tgctggatcc cgcgcgacgg ctgggccgat tatcgcactc gcagtcgcga agagttaatc
    63661 gagcgcatac atcagtttgg tgttgccggg ctgggcggtg caggattccc gacaggcgtt
    63721 aaattgcagg gtggcggaga taagattgaa acgttgatta tcaacgcggc tgagtgcgag
    63781 ccgtacatta ccgccgatga ccgtttgatg caggattgcg cggctcaggt cgtagagggt
    63841 attcgcattc ttgcgcatat tctgcagcca cgcgaaattc ttatcggcat tgaagataac
    63901 aaaccgcagg cgatttccat gctgcgcgcg gtgctggcgg actctaacga tatttctctg
    63961 cgggtgattc caaccaaata tccttctggc ggtgctaaac aattaaccta cattctgacc
    64021 gggaagcagg ttccacatgg cgggcgttca tccgatatcg gcgtattaat gcaaaacgtc
    64081 ggcactgctt atgcagtgaa acgtgccgtt attgatggcg agccgattac cgagcgtgtt
    64141 gtaaccctga ctggcgaagc aatcgctcgc ccgggcaacg tctgggcacg gctggggacg
    64201 ccagtgcgtc atttattgaa tgatgccgga ttctgcccct ctgccgatca aatggtgatt
    64261 atgggtggcc cgctaatggg ctttaccttg ccatggctgg atgtcccggt cgtaaagatt
    64321 accaactgtc tgttggctcc ctctgccaat gaacttggcg aaccacagga agaacaaagc
    64381 tgcatccggt gtagcgcctg tgctgacgcc tgccctgcgg atcttttgcc gcaacagttg
    64441 tactggttca gcaaaggtca gcaacacgat aaagctacca cgcataacat tgctgattgc
    64501 attgaatgtg gggcttgcgc gtgggtttgc ccgagcaata ttcccctggt gcaatatttc
    64561 cgtcaggaaa aagctgaaat tgcggctatt cgtcaggaag aaaagcgcgc cgcagaagcc
    64621 aaagcgcgtt tcgaagcgcg ccaggctcgt ctggagcgcg aaaaagcggc tcgccttgaa
    64681 cgacataaga gcgcagccgt tcaacctgca gccaaagata aagatgcgat tgctgccgct
    64741 ctggcgcggg tgaaagagaa acaggcccag gctacacagc ctattgtgat taaagcgggc
    64801 gaacgcccgg ataacagtgc aattattgca gcacgggaag cccgtaaagc gcaagccaga
    64861 gcgaaacagg cagaactgca gcaaactaac gacgcagcaa ccgttgctga tccacgtaaa
    64921 actgccgttg aagcagctat cgcccgcgcc aaagcgcgca agctggaaca gcaacagg
//
LOCUS       DAZGSP010000011        63001 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZGSP010000011 DAZGSP010000000
VERSION     DAZGSP010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.011"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(52..1911)
                     /gene="gss"
                     /locus_tag="ACHK74_000831"
     CDS             complement(52..1911)
                     /gene="gss"
                     /locus_tag="ACHK74_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFP0219695.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2116..2982
                     /gene="yghU"
                     /locus_tag="ACHK74_000832"
     CDS             2116..2982
                     /gene="yghU"
                     /locus_tag="ACHK74_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFP0219696.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3011..3092)
                     /locus_tag="ACHK74_000833"
                     /pseudo
     CDS             complement(3011..3092)
                     /locus_tag="ACHK74_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3105..3353)
                     /gene="hybG"
                     /locus_tag="ACHK74_000834"
     CDS             complement(3105..3353)
                     /gene="hybG"
                     /locus_tag="ACHK74_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFP0219697.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3366..3707)
                     /gene="hypA"
                     /locus_tag="ACHK74_000835"
     CDS             complement(3366..3707)
                     /gene="hypA"
                     /locus_tag="ACHK74_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP0219698.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3700..4188)
                     /gene="hybE"
                     /locus_tag="ACHK74_000836"
     CDS             complement(3700..4188)
                     /gene="hybE"
                     /locus_tag="ACHK74_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFP0219699.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4181..4675)
                     /gene="hybD"
                     /locus_tag="ACHK74_000837"
     CDS             complement(4181..4675)
                     /gene="hybD"
                     /locus_tag="ACHK74_000837"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFP0219700.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4675..6378)
                     /gene="hybC"
                     /locus_tag="ACHK74_000838"
     CDS             complement(4675..6378)
                     /gene="hybC"
                     /locus_tag="ACHK74_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFP0219701.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6375..7553)
                     /gene="hybB"
                     /locus_tag="ACHK74_000839"
     CDS             complement(6375..7553)
                     /gene="hybB"
                     /locus_tag="ACHK74_000839"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFP0219702.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7543..8529)
                     /gene="hybA"
                     /locus_tag="ACHK74_000840"
     CDS             complement(7543..8529)
                     /gene="hybA"
                     /locus_tag="ACHK74_000840"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFP0219703.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8532..9650)
                     /gene="hybO"
                     /locus_tag="ACHK74_000841"
     CDS             complement(8532..9650)
                     /gene="hybO"
                     /locus_tag="ACHK74_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFP0219704.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9839..10126)
                     /gene="yghW"
                     /locus_tag="ACHK74_000842"
     CDS             complement(9839..10126)
                     /gene="yghW"
                     /locus_tag="ACHK74_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFP0219705.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10245..11132)
                     /gene="yghX"
                     /locus_tag="ACHK74_000843"
     CDS             complement(10245..11132)
                     /gene="yghX"
                     /locus_tag="ACHK74_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFP0219706.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11289..12329
                     /gene="gpr"
                     /locus_tag="ACHK74_000844"
     CDS             11289..12329
                     /gene="gpr"
                     /locus_tag="ACHK74_000844"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFP0219707.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12369..12863)
                     /gene="yqhA"
                     /locus_tag="ACHK74_000845"
     CDS             complement(12369..12863)
                     /gene="yqhA"
                     /locus_tag="ACHK74_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFP0219708.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13054..13938
                     /gene="yghA"
                     /locus_tag="ACHK74_000846"
     CDS             13054..13938
                     /gene="yghA"
                     /locus_tag="ACHK74_000846"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP0219709.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14210..14635)
                     /gene="exbD"
                     /locus_tag="ACHK74_000847"
     CDS             complement(14210..14635)
                     /gene="exbD"
                     /locus_tag="ACHK74_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFP0219710.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14642..15376)
                     /gene="exbB"
                     /locus_tag="ACHK74_000848"
     CDS             complement(14642..15376)
                     /gene="exbB"
                     /locus_tag="ACHK74_000848"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP0219711.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15628..16815
                     /gene="metC"
                     /locus_tag="ACHK74_000849"
     CDS             15628..16815
                     /gene="metC"
                     /locus_tag="ACHK74_000849"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFP0219712.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16955..17614
                     /gene="yghB"
                     /locus_tag="ACHK74_000850"
     CDS             16955..17614
                     /gene="yghB"
                     /locus_tag="ACHK74_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFP0219713.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17654..18553)
                     /gene="yqhC"
                     /locus_tag="ACHK74_000851"
     CDS             complement(17654..18553)
                     /gene="yqhC"
                     /locus_tag="ACHK74_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFP0219714.1"
                     /translation="MKREEICRLLADKVNKLKIKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18747..19910
                     /gene="yqhD"
                     /locus_tag="ACHK74_000852"
     CDS             18747..19910
                     /gene="yqhD"
                     /locus_tag="ACHK74_000852"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFP0219715.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLIREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20015..20842
                     /gene="dkgA"
                     /locus_tag="ACHK74_000853"
     CDS             20015..20842
                     /gene="dkgA"
                     /locus_tag="ACHK74_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFP0219716.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21042..21968
                     /gene="yqhG"
                     /locus_tag="ACHK74_000854"
     CDS             21042..21968
                     /gene="yqhG"
                     /locus_tag="ACHK74_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFP0219717.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22019..22276
                     /gene="yqhH"
                     /locus_tag="ACHK74_000855"
     CDS             22019..22276
                     /gene="yqhH"
                     /locus_tag="ACHK74_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFP0219718.1"
                     /translation="MKTIFTVGVVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22319..24538)
                     /gene="ygiQ"
                     /locus_tag="ACHK74_000856"
     CDS             complement(22319..24538)
                     /gene="ygiQ"
                     /locus_tag="ACHK74_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFP0219719.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24649..26061)
                     /gene="ftsP"
                     /locus_tag="ACHK74_000857"
     CDS             complement(24649..26061)
                     /gene="ftsP"
                     /locus_tag="ACHK74_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFP0219720.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26136..26873)
                     /gene="plsC"
                     /locus_tag="ACHK74_000858"
                     /gene_synonym="parF"
     CDS             complement(26136..26873)
                     /gene="plsC"
                     /locus_tag="ACHK74_000858"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFP0219721.1"
                     /translation="MLYIFRLIITLIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27106..29364)
                     /gene="parC"
                     /locus_tag="ACHK74_000859"
     CDS             complement(27106..29364)
                     /gene="parC"
                     /locus_tag="ACHK74_000859"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFP0219722.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29910..30392)
                     /gene="ygiV"
                     /locus_tag="ACHK74_000860"
     CDS             complement(29910..30392)
                     /gene="ygiV"
                     /locus_tag="ACHK74_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP0219723.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30445..30837)
                     /gene="ygiW"
                     /locus_tag="ACHK74_000861"
     CDS             complement(30445..30837)
                     /gene="ygiW"
                     /locus_tag="ACHK74_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFP0219724.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            30989..31648
                     /gene="qseB"
                     /locus_tag="ACHK74_000862"
     CDS             30989..31648
                     /gene="qseB"
                     /locus_tag="ACHK74_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFP0219725.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31645..32994
                     /gene="qseC"
                     /locus_tag="ACHK74_000863"
     CDS             31645..32994
                     /gene="qseC"
                     /locus_tag="ACHK74_000863"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFP0219726.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(33040..33372)
                     /gene="ygiZ"
                     /locus_tag="ACHK74_000864"
     CDS             complement(33040..33372)
                     /gene="ygiZ"
                     /locus_tag="ACHK74_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFP0219727.1"
                     /translation="MLKHKIQTIFEALLYIMLTYWLIDAFFAFNKYDWMLESGDNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLF
                     WGRFIFCY"
     gene            33691..34272
                     /gene="mdaB"
                     /locus_tag="ACHK74_000865"
     CDS             33691..34272
                     /gene="mdaB"
                     /locus_tag="ACHK74_000865"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFP0219728.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            34303..34617
                     /gene="ygiN"
                     /locus_tag="ACHK74_000866"
     CDS             34303..34617
                     /gene="ygiN"
                     /locus_tag="ACHK74_000866"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFP0219729.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(34665..36557)
                     /gene="parE"
                     /locus_tag="ACHK74_000867"
     CDS             complement(34665..36557)
                     /gene="parE"
                     /locus_tag="ACHK74_000867"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFP0219730.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(36586..37167)
                     /gene="yqiA"
                     /locus_tag="ACHK74_000868"
     CDS             complement(36586..37167)
                     /gene="yqiA"
                     /locus_tag="ACHK74_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFP0219731.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(37167..37994)
                     /gene="cpdA"
                     /locus_tag="ACHK74_000869"
     CDS             complement(37167..37994)
                     /gene="cpdA"
                     /locus_tag="ACHK74_000869"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFP0219732.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(38019..38441)
                     /gene="yqiB"
                     /locus_tag="ACHK74_000870"
     CDS             complement(38019..38441)
                     /gene="yqiB"
                     /locus_tag="ACHK74_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFP0219733.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(38442..39071)
                     /gene="nudF"
                     /locus_tag="ACHK74_000871"
     CDS             complement(38442..39071)
                     /gene="nudF"
                     /locus_tag="ACHK74_000871"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFP0219734.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            39276..40757
                     /gene="tolC"
                     /locus_tag="ACHK74_000872"
     CDS             39276..40757
                     /gene="tolC"
                     /locus_tag="ACHK74_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFP0219735.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            40905..41576
                     /gene="ygiB"
                     /locus_tag="ACHK74_000873"
     CDS             40905..41576
                     /gene="ygiB"
                     /locus_tag="ACHK74_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFP0219736.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            41582..42742
                     /gene="ygiC"
                     /locus_tag="ACHK74_000874"
     CDS             41582..42742
                     /gene="ygiC"
                     /locus_tag="ACHK74_000874"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP0219737.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(42780..43568)
                     /gene="ygiD"
                     /locus_tag="ACHK74_000875"
     CDS             complement(42780..43568)
                     /gene="ygiD"
                     /locus_tag="ACHK74_000875"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFP0219738.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            43711..44484
                     /gene="zupT"
                     /locus_tag="ACHK74_000876"
     CDS             43711..44484
                     /gene="zupT"
                     /locus_tag="ACHK74_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFP0219739.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(44542..44712)
                     /gene="yqiD"
                     /locus_tag="ACHK74_000877"
     CDS             complement(44542..44712)
                     /gene="yqiD"
                     /locus_tag="ACHK74_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFP0219740.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(44973..45626)
                     /gene="ribB"
                     /locus_tag="ACHK74_000878"
     CDS             complement(44973..45626)
                     /gene="ribB"
                     /locus_tag="ACHK74_000878"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFP0219741.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(45730..45878)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            46000..46290
                     /gene="ubiK"
                     /locus_tag="ACHK74_000879"
     CDS             46000..46290
                     /gene="ubiK"
                     /locus_tag="ACHK74_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFP0219742.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            46573..47124
                     /gene="ygiL"
                     /locus_tag="ACHK74_000880"
     CDS             46573..47124
                     /gene="ygiL"
                     /locus_tag="ACHK74_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFP0219743.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
     gene            47184..49688
                     /locus_tag="ACHK74_000881"
     CDS             47184..49688
                     /locus_tag="ACHK74_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0219744.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEVAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSFSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            49704..50453
                     /gene="yqiH"
                     /locus_tag="ACHK74_000882"
     CDS             49704..50453
                     /gene="yqiH"
                     /locus_tag="ACHK74_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0219745.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            50455..51519
                     /gene="yqiI"
                     /locus_tag="ACHK74_000883"
     CDS             50455..51519
                     /gene="yqiI"
                     /locus_tag="ACHK74_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0219746.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(51562..51762)
                     /gene="glgS"
                     /locus_tag="ACHK74_000884"
     CDS             complement(51562..51762)
                     /gene="glgS"
                     /locus_tag="ACHK74_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFP0219747.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            52031..52660
                     /gene="yqiJ"
                     /locus_tag="ACHK74_000885"
     CDS             52031..52660
                     /gene="yqiJ"
                     /locus_tag="ACHK74_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFP0219748.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            52687..54348
                     /gene="yqiK"
                     /locus_tag="ACHK74_000886"
     CDS             52687..54348
                     /gene="yqiK"
                     /locus_tag="ACHK74_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFP0219749.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            54577..54636
                     /locus_tag="ACHK74_000887"
     CDS             54577..54636
                     /locus_tag="ACHK74_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP0219750.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            54952..55011
                     /gene="ibsE"
                     /locus_tag="ACHK74_000888"
     CDS             54952..55011
                     /gene="ibsE"
                     /locus_tag="ACHK74_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFP0219751.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(55131..56564)
                     /gene="hldE"
                     /locus_tag="ACHK74_000889"
     CDS             complement(55131..56564)
                     /gene="hldE"
                     /locus_tag="ACHK74_000889"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFP0219752.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(56612..59452)
                     /gene="glnE"
                     /locus_tag="ACHK74_000890"
     CDS             complement(56612..59452)
                     /gene="glnE"
                     /locus_tag="ACHK74_000890"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFP0219753.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIVHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(59475..60776)
                     /gene="ygiF"
                     /locus_tag="ACHK74_000891"
     CDS             complement(59475..60776)
                     /gene="ygiF"
                     /locus_tag="ACHK74_000891"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFP0219754.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYHDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            61018..61638
                     /gene="ygiM"
                     /locus_tag="ACHK74_000892"
     CDS             61018..61638
                     /gene="ygiM"
                     /locus_tag="ACHK74_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFP0219755.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            61702..62940
                     /gene="cca"
                     /locus_tag="ACHK74_000893"
     CDS             61702..62940
                     /gene="cca"
                     /locus_tag="ACHK74_000893"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFP0219756.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAIASWKEQRCPKPD"
BASE COUNT        15883 a        16566 c        15516 g        15036 t
ORIGIN      
        1 tccccgtact gattattctt cataatcagt acggggttgc tacaacatgt attacttttt
       61 caccacaatt aacggttcaa tatcactctc ttttttgatg accagtgatt catcaccgcg
      121 caaacacgtc ccaccgtagt tgccgccaac ggtgaaggta catacctgaa tgtatttacc
      181 gtccactttc ggcaaacacc acagttgctg atagatgttt ttctgctcgg caaatttacc
      241 gctggttttg tccagcacct cttcatgatg gctgacgagg tcgatattgc tgccacagcg
      301 accggcgatc ggtttcactg cgtaacctgt tttcaccagt tcatcattaa cagtgaaatc
      361 ggtatccagc aggtaacgat ggtgcgggaa cagcgaccag aggatcggca gaatcgcttt
      421 gttgccgggg atcaccgtcc acagcggctc aaagaccagc acttccgggc gcagcaatac
      481 gtcgataaga cgcacttcgt tttgcggatg accggtacgg attggcaccg cagcaaactc
      541 acggtcgcta acttcacgaa tctgatcaaa cgcggtttcc cacgcccagg ttttccacac
      601 gcagttaacc agccgtcctt ccccatcaat cagttgcccg gcagcatccc agcccagttc
      661 atccagcccg cgcaagatac gcgtttcaaa gcccgcctgg tgcagcgcct gctccataaa
      721 ctgcgcgtga tagttttcct cgatatcttt gtcctgcatg atatggacaa acggacgtgc
      781 acgactgtgt ttccaggcac cagccagttc gttaatcagc ccttccgccg gattgaagcc
      841 gttgccttta tagccctgct ccgcccaacg ttcgaggatc aagcccgctt cggtatgaca
      901 tgaggcagag tcggcgttgt attcgtaaac cttcaggcca cgctcatcca tgcagaaatc
      961 catacgacca gtgatcatat ggtgacggcg acgctgccag gagagacgca aacgtggcca
     1021 gaggattttc gggatgtcga acagcgccag caggttgtca tctttcagca ccttgtcggt
     1081 tgcgtgaaga tacatcaggt gcagctcgtt ggtggcttta attagctcct gctcggcact
     1141 ctcggtaatg gtgtagtaat gataaggatc ctgattgatc acctgaccgt tggcctgcac
     1201 ataggcgttt tgcagcggat ctttttcatc cagccattta ccgtcaaact ggcctttgtt
     1261 ttccaggcgc gctccgctga ttttcagcag ctcgcctgca atttccggct gcggtaagct
     1321 gtattcagta tcttccgtct ggatcatcca gcccagaatg gtggtgtcat caaaagtgtc
     1381 tttcagggta tagcagccgt tttcgaccac catctccagc tcacgcgtcc actgttgccc
     1441 ttgcggcaac ggggaatgaa tcacgttctg ttccgcaata cggactttgt tgccatgcaa
     1501 ttgggtaatg atggcgacat ggccagtgtc tttaaattca ccgcctttat cccagataag
     1561 aagcgcaccc gcgaccggcg cacgcggcga gccgttagga aatgcctgca atggcaggat
     1621 gttgtcatta accacttctc gcaggaagcg cagcgagaaa atctcccacg ccatacccac
     1681 gtcagtaaag accacaccgt aattcagaaa gagaaaacgg cgagcaaatt caacgcattg
     1741 ccacttgtgg cccatatatt cgtcgtcgat atagctacgg aatacggcgt catcttcgta
     1801 ttcctgcgga tcgagagaac tgtaatctga agagtagatt gctaccccac ctggggcgta
     1861 gcccaataat gtcccgaacg gggcatcctg gctggtcgtt cctttgctca tcactttacc
     1921 tttaacaata caacctaagc agttggcgta attgttgtgc tctgattacc tgctcagcga
     1981 cattaaccgg acagaggtca gactaatcat acacctcatc gcagcggctg gcgcaaaggg
     2041 ttaaaaaatt cacattctgt acagcaagtg acctgctaca ctgcttcaac actaccactc
     2101 agaaggcaac tcactatgac agacaatact tatcagcccg cgaaagtctg gacgtgggat
     2161 aaatccgctg gcggcgcgtt cgccaatatc aatcgcccgg tttctggtcc gacgcatgaa
     2221 aaaacgctgc ccgttggcaa acacccattg caactttatt cgctgggaac gccgaacggt
     2281 cagaaagtaa cgattatgct tgaggagctg ctggcgctgg gcgttactgg tgcagagtac
     2341 gacgcctggc tgattcgtat tggcgatggc gatcaattct ccagcggctt tgtcgaagtg
     2401 aacccaaact cgaagatccc ggcgctgcgc gatcatacgc ataatccgcc gatccgcgtg
     2461 tttgaatctg gttcgatcct gctttatctg gcggagaaat ttggctactt cctgccgcag
     2521 gatttggcaa agcgtactga aacgatgaac tggctgttct ggttacaggg cgcggcaccg
     2581 ttcctcggcg gtggttttgg tcacttttac cattacgcac cggtaaagat tgagtacgcc
     2641 atcaaccgct ttaccatgga agccaaacgt ctgctcgacg tgctggataa gcaactggcg
     2701 cagcataagt ttgttgcggg cgatgagtac accattgcgg atatggcgat ttggccgtgg
     2761 tttggcaacg tggtgttagg tggtgtgtat gatgccgctg agtttcttga tgcgggcagt
     2821 tataagcatg tacaacgctg ggcgaaagaa gtaggcgaac gtccggcggt gaaacgtggg
     2881 cgtattgtta accgcaccaa cggaccgctg aatgagcagt tgcatgagcg ccatgacgcc
     2941 agtgatttcg agacgaatac ggaagataag cgtcaggggt aagggttggt gttcgtcgca
     3001 gcaagccatc caggccggat aaggcgttca cgccgcatcc ggcaatcgtg cacaatgcct
     3061 gatgcgacgc tgccgcgtct tatcaggcct acgaaagcta atcatcacgc gctggtaatg
     3121 tcgtaatcca tttggcgcag tgcgtctaat gtggctttgg cttcatcttc gtcgatgatg
     3181 ctcatggcaa atccgacgtg taccagcacc cactggccca gtagatcggc agggttacct
     3241 tcacaaatca gggcgatatt cacgtcgcgc ttgataccac atacttcaac ctgcgcaagc
     3301 tggtgaatat cttcaccgac agccagcacc tggcctggaa cgccaataca cattattaac
     3361 tccggttatt caacttcaat acttttgacg atcagcgaat cgccggtatc gacacgcaac
     3421 cgctcgccgt gacagagcgg acactgcgca tcgtgctgat gaatctccac cacctggctg
     3481 caatcccagc accaggcctg ggcgggttta tagacgatat gtaaatcgca cccttgcgcc
     3541 accgttccgt ggcagacaat ttcaaaacta aaacggacgg cgctctcctc aacgcaggag
     3601 agcgcgccaa tttccagcca cacggcggtg acgcgcttaa catcgtgctg ctccgcctgc
     3661 cgttggataa tttcaacggc gctctggcaa agagacaact catgcatttt cgccactcct
     3721 gcgaccaaac agcagggcgc gacgccctgc gtgtggtaca tccggattcg tgactggcag
     3781 cgaaaggatc attcgtgcgc agtcatccgt caggcgttgg ccctcttcaa tcgacatgct
     3841 gtgcgaaagc ggcgacatca gcgaacagga gagatattgc gaaacaccgt ccagttcgcc
     3901 aacggtaaag gtcatagtgc catacggcag ttgcagacca attttttcac tcactttgcg
     3961 cagcggccag agttgatccg ggccggggaa gataactgca ctcagcatcc acggggtgat
     4021 cacacacccc gtccactgac cttcgaacag cgtaaaatca gaaacataca ccggcattga
     4081 aggatgcaga aaagagagat cgtgcatcga acgccgggca atttcttcaa acgctgcctg
     4141 tacttgcgcc ttcggggagg tctggaaacc tgctatctct tcagtcatga atcgcctccc
     4201 gtgggatggc ttccacgcca gattcacgca gcgcagccag aacctgctca agcgcaggtt
     4261 caatcattgc ttcaaccgtc ggcgttaagc cgatgtgtgg ctccagcgat tccgggatca
     4321 cgccgaccag ggtcagcttt ttcggaaact caccggtgaa gcgcagggcc gacaagacgt
     4381 cggccaggcc aagctgatgc ggagagattt tgttggtaaa caacgccgga acttcttcat
     4441 cgcgcaggat catcatcgtt cccggcgcgc tcttcttcga cacaatggca tccgcaataa
     4501 tcagatgatc gcgatttgcc atgtcgccaa gcagctccat tcccgccgtg ccgccatcga
     4561 ggatctcaac ataatccggc agaatgtatc gttgctctaa cgcttcgaca atccgcacac
     4621 cgatggcttc atcggtcagc aaaatattgc cgacccctaa gactaaaata cgcattacag
     4681 aaccttcact gaaaccactt cgttgccgtc agcatccact acgtgtaccg cacaggccat
     4741 gcacgggtcg aaggagtgaa tggtacgcac cacttccagc ggtttattcg gatcggcaac
     4801 cggtgtaccc accagcgact gctcgtaagg accgacgtca tcattgaagt tacgcggacc
     4861 agagttccag gttgatggaa caaccgcctg gtagttactg atgataccgt ctttgatcac
     4921 catccagtga gagagcatac cgcgcggcgc ttcgaggaag ccaacacctt tgaactcacc
     4981 cgttgccgga atgttcggct tcacaaaggt ggtatgatcg cctttaccga tattggtgat
     5041 cagtgcactg tattgatttt gcaggatatc ctgcaattcg cagcagtgaa cggtacgacc
     5101 aataatacgg cccagcgtag agtgcagttg cgccacttca agcgtgttac cagtcagttt
     5161 ctgataaatc gcaacgattt cattcagttt gttctgggta gactcgcgtc ctgccgccag
     5221 tttcaccagc atgttagcca gcggacccac ttctaccgtt ttgccgtaga aagtcggtga
     5281 tttcacccag gaatatttcc cgtcgtcaga ccaaccatca taagccggaa tggtggtacc
     5341 ttcccacggt gcctgcggcg cttcgtcttt ataccaggag tgcttcgcgc tttcctgaat
     5401 gcctttgatc aggtattcat cggaatgaga agtgatcgga cgatacgagg acagatccgc
     5461 attctcaatg tagccgcccg ggaacaggaa gctgccgttt ttactgtcgg tcgggaattc
     5521 cggcacgctc aggtagttca ccgcaccttt accgcgcgtc agccattccg ggtagaacgc
     5581 ggcaataact gcggtatcaa ccttataaac ctgttcaaca aagtcgctca gcttgtcgat
     5641 gaaagacttg atgtacatca ggcgctcaag gttcagcacg cccaaaccgt cgaggttgat
     5701 cgggttcgcg acaccaccta ccgccaggtt ctgaatgtgc ggcgttttac cgcccagcag
     5761 cgccacgacg cggttagcgt cacgctggca ctccagcgcc tgcaggtagt gcgctaccgc
     5821 aatcaggttc acttccggcg gcagtttcat cgccgggtga ccccagtagc cattggcgaa
     5881 aatacccaac tgaccgctgg caaccagatc tttgatcttg ttctgaactt tggtgaactc
     5941 ttccggactg ttcaggtgcc aggtcgaaac gcctttcagc atttcggagg ctttggttgg
     6001 gtcagcttgc agtgcagaag tgatgtccac ccagtccagc gccgaaagct gatagaaatg
     6061 aacaatatgg tcatgcgtgg tgtgcgcagc cagaatgatg ttacggatgt attgcgcgtt
     6121 aaccggaacg tcgatattca gcgcactttc tgccgcacga acggaagaca gcgcgtgagt
     6181 ggtagtacat acgccacaga tacgttgcac aatcatccat gcatcgcgcg gatcgcggtt
     6241 tttcacgatc tcttccatgc cgcgccacat ggtaccggaa gcccatgctt tcgaaacgac
     6301 gccattttcg atttcgcaat cgatgcgtaa atgcccctca atacgggtta ccggatcaat
     6361 agtaattctc tggctcatgc tttgctcgcc tcatgacgat tatgatcgtt ttgttttaaa
     6421 ggaggaagta tcggcagtag acgaatgagt acgatgtaag cgcaaatctc aatagccaca
     6481 aaaccaatag aaatcaacag ttcttcccag gtcgggaagt aggcgtaacc gccgcccggg
     6541 ttgaatgcca ccagcgaata ggtcagacgc caggttgcac aacctaacag tgcgctcagt
     6601 gctgacagga acagcatgcg ggaatcatta cgcagcttcg ccacacgcag aacgaccagc
     6661 gggaagagca tcagcaggac ttcaatccag aacatcacgg agtagaagtc accggcaaac
     6721 gctaacgaca gcttgtcgcg atagataagc tcgccaaagc gcagcacgat gaaaatcgcc
     6781 agcaacacac tgatggtgtt ggtcagctta acaaacagac tcttttcatc cggaccgttg
     6841 ccacgcagac ccgcctgcac cagcgaacct tcaaagatga caatcgagaa gcccatgatg
     6901 aacgccgtca gcagcgagaa cagcggcaac atttcatagc tctgccacaa cggatgcacc
     6961 ttgtagcccg ccgagatcat cagcgacccc attgaagact ggtgcatggt cggcagcagc
     7021 gcaccgagcg cgatgatgaa gaacatcacc ttgtttagtc gctgtagcga caccttccac
     7081 cccagacgtt caaacagtgc cggagcaaac tccagtgcca tcacgccgat atagatggtc
     7141 atacagaccg ccgtctcgaa cagtaccgag ttcacgttga agtggcccgg aatgtagaag
     7201 tacggcaggt tccagtagcg acccacgtcg atagtgatcg acaagccacc cagtgagtaa
     7261 ccaaacagac tcgccaacag cgccggacgc accagcggat ggtattgccc acggttaaag
     7321 acgtataccg cccacgccag cgcccagccg ccacaggcaa agccggtgcc aatcagcagg
     7381 tcaaacgcga tccacacgcc ccacgggaag ccgccgttca ggtcagagac agagcccaga
     7441 ccgaacacca gacgcttcac aatcaggagc atacagatga cgattaacgg tccaaaaatc
     7501 atgaccggtt tactgatgat tttgccgccc agcggttgtg gatcatgact catgatcgtc
     7561 tcctccgtcg tgatggtcgt ttttggtgtt gcgacgaacc agcacggtta agcccgccag
     7621 cacagccagt ggtagcatca tgcctttata cagggtgtgt tgaatatttt cggaacgcgc
     7681 accggtagaa agatcgtcca gtttcggcag gtcgagattt tcataaggca cacccgtcag
     7741 taccagaacc tgagtaccgc cgccctcttt ctcgccgtac agatgcggat aatatttcgg
     7801 caccgtatgc aggtaagtgt cgccagattt cagcgtctga cgcggatagt ggtattcgct
     7861 gccaggcttc agcgccagac gttttttcgc ctccgccatc agctcttcac gcgtaccgaa
     7921 aatcaccgcg cccgccgggc acacttctac gcagccaggc agaccgcctt tatcgagacg
     7981 ttccacacct ttctggttgc acagctcgca cttatgcagc gcaccaaacg ggttgttgta
     8041 gtcgtacttc ggcacgttgt acggacaggc gaccatgcag taacggcagc cggtgcacac
     8101 gtctttgtcg taatggacaa tgccggtttt cggatctttt ttcagtgcag agaccgggca
     8161 cacagagaca cagttcggat cgacgcagtg catacactgt ttcttaatgt acgcgtagcc
     8221 gttctcctcc tggtctttgt tgacccctgt gccgctggtc cacacctgaa tgatgttatt
     8281 ggtatacggc gacagtttgt cgttgttcga ccaggtctgt tccccttgcg ggttacgttc
     8341 agggaagttg atatcctgac acttggtgac gcaagcctgg cagcctacgc acaaggtcga
     8401 gtcgtacaac atccccagcg atcccggaat tggcgggcgg ttttcagcag ccgcatgact
     8461 gacagacggc agcgcgcccg tcagcaatgc cccgcaggag gctgctttaa taaaattacg
     8521 tctgttcacg gttattctcc ccgtgagtca gcgttatctt tcttttgctg acgacccagt
     8581 tcacgcaccg ccatcacgct gacaccggca acgagcccaa ccacaccgcc gagcaaacca
     8641 atagcgcctg cagagacgtt gccgccctct ttagcgttaa catccggttt ctgtgaacgc
     8701 ggagtctgat tttcgacgtt ggcaagctga tggatgcctt tatggaagcc gataccttct
     8761 tcgttacagc catagcatgg gtgaccaatc gctaccggcc acacgccgcc aacatcgcag
     8821 aattgcagcg ttgagcagtt gccgtaagtt tctggccctt tacagccgag gtggtacagg
     8881 caccagcctt cacggtggcc ttcatcaccg aactctttgg caaaacgacc agcatcgaag
     8941 tgcgggcgac gttcgcagtg ttcgtgaatc agacggccat aggcgaaggt cggacgattt
     9001 ttgtcatcca gtttcggcgg tttgccgtaa gtgatgatgt gcgcaacggt cgcgaggaag
     9061 ttgtgcgggt tcggcgggca gcccggaata ttgataacgg ttttgcctgg cagaacttct
     9121 tgcaggctga ctgcgccagt tgggttaact ccagctgcgg caacaccgcc ccacgcagag
     9181 caggaaccga tagcgataat cgctgctgcg ccttctgccg ctttgcggat gtgatccaca
     9241 atcggctcac cagcaaccat gcaataaata ccgttatctt ttaatgggat ggaaccatcc
     9301 acaaccaaca catactgccc tttgtacttc tcaagtgcgt tatgtttgtt ctcttcgacc
     9361 tgatgaccga aggcggcgga aagcacttcg tgatactcca gagagatagt ctccagcacg
     9421 aggttttcta ccgttggatg cgttgcacga agcagagatt ccgtacaacc ggtgcactcc
     9481 tgcgcgccaa tccagataac tggcggacgc tgcgggttag taaccgattc ggccatctct
     9541 gcagcggctt tgctacttaa ccccatggtg gcggctaatg ctgcacaaag cttcatgaaa
     9601 tcacgacggt taatgccgtg agaatggatg agggtgttat ctccagtcat ttatagttat
     9661 tccgttgcga agacctggca tatattttgc ctcaatcgca aaatcaataa tgcgatcgat
     9721 gcgccattta ccacacattt attatggtta tcgatatcat gatactgcgg cggggcgcta
     9781 aaacgaaggg aagaagcatt agtgtaatta attaaagcaa gataatacgt atgtttgatc
     9841 aattttcgag gcgataaccg gccataaaaa agcgcaacgt agaacaggaa ttattctcac
     9901 gaaagaaatc cattaccatc tctttatcca gtccatagcg gcgcgtcaga atacccgctt
     9961 cccaggcgct aagctggcga tctccggtct tttcgtacat ccggtgtgag tagcctaata
    10021 caaaaccgcg tttataatcg gcacagaatt ttgtcacatt aaccgcactg tcggcatgcg
    10081 ttgcttttaa tcccgccatt aagcctttac caaaatggtt attcatcgtt cacctcattc
    10141 gatatatatc gccatatata gcgatatttt aggcaaagaa cgacttttgc gcacgctatt
    10201 ttttcatgct tgtttaagac gtaatccagt aagagataaa cctatcagga gagatattta
    10261 tcgaaccatt tcagtgtcct ttgccaggca agatcggcgg cagatttgtc ataacggggc
    10321 gtggaatcat tatggaatcc gtgattaacc cccggataga tatacgcctc ataaacctta
    10381 ttattggctt tcaacgccgc ctcgtaagca ggccagccct cgttgattcg ggtgtccagt
    10441 tccgcgaagt ggagtagtaa aggcgcttca atcttcgcca catcggcagt gggtgcctga
    10501 cgaccataaa acggcaccgc gcaggccagt tccggatacg cgacagccgc cgcgttcgat
    10561 acgccaccgc catagcaaaa tccggtaata cccactttgc ctgccgcttg cggatagcgt
    10621 tgcataaact caattgcggc aaagaaatca ttcatcagtt tggttggatc aacctgctgt
    10681 tgcagctcac gacctttatc atcatttccc ggataacctc caacggaact taagccgtca
    10741 ggtgccaggg cgatataccc cgccttcgcc actcgccgtg ccacatcttc gatatacgga
    10801 ttcagtccac gattctcatg caccactacc acggctggcg ttttgccgct catttttgcg
    10861 ggtttcacca gataaccccg tacctcgccg tgaccatttg gcgaaggata cgtgatgtac
    10921 tcagcaacaa tctccgggtc ggtaaactct acctgagtcg ccagcgcata atttggcttg
    10981 agcaaatcaa acaacgctaa tgccgtcatc ccgcccaccg catacttcgc cgcaaggttg
    11041 aggaactcac gtttcgagat tttcccgtga gcgtaatagt cgtagtaatc caacaactct
    11101 tgtgggaaat ctttggcggt taaacgcggc atcgttgcac tcctcaactg gtgttttttt
    11161 aagcaaagca taagcacgtg tttttgccca gtttttcgtc actctgtgag ccagactacg
    11221 ggatacgcgc tggcgaatcg ctaaactaga aacattgttt cgaaattgaa cggtggaaag
    11281 gagaggtcat ggtctggtta gcgaatcccg aacgttacgg gcagatgcaa taccgctatt
    11341 gcggaaaaag cggtttacgc ctgcccgcgt tatcgctcgg tttatggcac aatttcggtc
    11401 acgttaacgc gctggaatca cagcgtgcga tcctgcgtaa agcgtttgat ttgggcatta
    11461 cgcactttga tttagccaac aattacgggc cgcctccagg aagcgcagaa gagaactttg
    11521 gtcgcctgct gcgggaggat tttgccgctt atcgcgatga actgattatc tctaccaagg
    11581 ctggctacga tatgtggccc ggcccttacg gctctggcgg ttcacgtaaa tacctgctcg
    11641 ccagcctcga ccaaagcctg aagcgtatgg ggcttgagta tgtcgatatc ttttactctc
    11701 atcgcgtcga tgaaaatacg ccgatggaag aaaccgcctc tgcgctggct catgcggtac
    11761 aaagcggtaa ggcgctgtat gtcgggatct cctcttactc gccagagcgg acgcaaaaaa
    11821 tggtcgagtt gctgcacgag tggaaaattc cgcttttaat tcatcaacct tcgtacaatt
    11881 tactgaaccg ctgggtggat aaaagcggcc tgctggatac cctgcaaaat aacggcgtgg
    11941 gctgtattgc ctttactcct ctggctcagg gattgctgac cggaaaatat ctcaacggca
    12001 ttccgcaaga ttcacggatg tatcgtgaag ggaataaagt tcgtggtctg acaccgaaaa
    12061 tgcttaccga agccaacctc aacagcctgc gcttattgaa tgaaatggca cagcagcgtg
    12121 gacaatcaat ggcgcaaatg gcgttaagct ggttgttgaa agatgatcgc gtgacgtcgg
    12181 tattgattgg tgccagccgc gcggagcaac tagaggagaa cgtgcaggcg ctgaataatc
    12241 tgacatttag caccgaggag ctggcgcaga ttgatcagca tatcgccgat ggcgagctga
    12301 atctgtggca ggcgtcgtcc gataaatgac ctgttaataa cgggcgcgcg aaccgcgccc
    12361 gaatcagatc agtgattatg acgagtcagt cggtcaagat agcccatcac aaatgcagaa
    12421 agcacaaacg tcagatggat aatgacgtac cacatcagtt tgttatccgg gacatttttc
    12481 gcatccataa agacgcgcag taggtgaatg gaagaaattg ccacaatcga cgctgctact
    12541 ttgtttttca gcgacgttgc gtccattttc cccagccagt tcagcttctc tttgttctcg
    12601 gagatatcca gctgtgagac gaaattctca taaccggaaa acatcaccat caccagcaaa
    12661 ccgccaacca gcgtcatatc caccagcgac agcaacacga ggatcaaatc tgattccgcc
    12721 atcgagaaga tattcggcag tacgtgaata atctcctgga agaacttcag cgccagggca
    12781 actaacgcca gcgaaaggcc aaagtacacg ggggcaagca gccagcgaga agcatacatt
    12841 gcattttcaa gaaaacgttc catacattcc tgtcgtgtag ttaaaacggg caacagtata
    12901 tcgcaattga gtaaacagca cgcaacagca aatcaacata tacgttaaac ctttgtcgca
    12961 taattctgag gtttagggat cttcctggtt cccttatcaa cgcatcgaga gcgtctaccc
    13021 ttaaaggacg ttgaacctga agggagaaaa acgatgtctc atctaaaaga cccgaccacg
    13081 cagtattaca ctggtgaata tcccaaacag aaacaaccga cgccaggcat ccaggcgaag
    13141 atgacaccgg taccggattg cggcgagaaa acctatgttg gtagcggtcg cctgaaagat
    13201 cgtaaagcac tggtgacagg gggcgattcc ggaataggtc gcgctgccgc catcgcttac
    13261 gcgcgtgaag gggctgacgt ggcgatcagt tatcttcccg tggaagaaga agacgctcag
    13321 gatgtgaaaa agatcattga agaatgcggt cgcaaagccg ttctgctgcc aggcgattta
    13381 agcgatgaga aattcgcccg ttcgctggtt cacgaagcgc acaaggcgtt aggcgggctg
    13441 gatattatgg cgctggtcgc cgggaaacag gttgccattc ctgatattgc agacctcacc
    13501 agcgaacagt ttcaaaagac ctttgccatt aacgttttcg cgctgttctg gctaacccag
    13561 gaagcgatcc ccctgctacc gaaaggtgca agtatcatca ccacttcgtc aatccaggca
    13621 taccagccaa gtccgcattt actggactat gcggctacga aggcggcgat tctgaactac
    13681 agccgtggcc tggcaaaaca ggtcgcggag aaaggtattc gggtgaatat tgtcgcgcca
    13741 ggccctatct ggacggcact gcaaatttct ggcggacaaa cgcaggataa gatcccgcag
    13801 tttggtcagc aaacgccgat gaaacgtgcg gggcaaccgg cggaactggc ccctgtatat
    13861 gtttatctgg caagtcagga gtcgagctac gtcaccgcag aagtgcacgg tgtgtgcggc
    13921 ggcgagcatt taggttaaaa aaatgcccgg ttgtgaaaag caaccgggca tcattgtgaa
    13981 ttcactctta cctgatgtgg cgaaaacgcc ttatccgcct acaaacgcat gaagattcaa
    14041 catgttgcaa tagattttgt aggcctgata agcgtagcgc atcaggcatt gagcacctaa
    14101 tgccggatgc ggcgtgaacg ccttatccgg cctacaaacg cacgaagatt caacatgttg
    14161 caatagattt tgtaggcctg atgagcgtag cgcatcaggc aattctactt tacttcgctt
    14221 tggcggtttc ttcgccgacc agacctatct tcaggtaacc cgcctgatgc agcgtatcca
    14281 ttaccttcat caacgtctcg taatcgacgg ttttatccgc tcggaagaag atggtggtgt
    14341 ctttcttgcc ttcggttaac gcattcaacg ccgtaatcat tgtttcatcg gtgaccggat
    14401 cgttaccgat aaacatcgag ttgtctgcct tcaccgacag ataaaccggt ttttccgggc
    14461 gcggctgcgg cgtgctggta gaagcaggca agttcacctt cacatctacc gtcgctaacg
    14521 gtgccgccac cataaagata atcagcagaa ccaacatcac gtcgataaac ggcgtcacgt
    14581 tgatatcatg catttcgccg ttatcgtcga ggttttcgtt aagatgcatt gccatcggat
    14641 attatcctgc gcgtaatttt tgtgcgacac gaaccggatg cgcagcggcg ctggcttcca
    14701 gatccaggtc acggctttgc agcaacaata cctgcgctgc aacatcaccc agcatcgctt
    14761 taaagccgcc aatctggcgt gcaaatacgt tatagataac gaccgcagga atcgctgcca
    14821 cgaggccgat tgccgttgct aacagagctt ctgcgatacc cggcgcaacg actgccaggt
    14881 tagtggtctg cgtttgcgcg ataccaataa agctgttcat gatgccccag accgtaccaa
    14941 acagaccaac gaacggagaa atcgcgccga tggttgccag atagccgtta ccgcggccca
    15001 tttgacgacc cactgcggcg acccgacgct ccagacggaa gctggtacgt tctttaatac
    15061 cttcgttatc gtcgctgcct tctgacagtt ccagctcatt ctgcgcttca ttgagcaaat
    15121 gcaggcttaa gcttttgcta ccaaaatcag cggcgatatc gttggcctgg tttaaggaac
    15181 gcgcttcagc cagcagttgc tgctcgcgct taaggcgacg cttctgattg aagaactcta
    15241 cgctcttact gaagaagatt gcccaggtga ctacggaggc caaaataagc ccaatcatca
    15301 cgcacttaac gacaatatcg gcgtgctgat acatacccca gacggaaagg tccgtctgca
    15361 ttaaattatt acccactgtg tatctccagg acgcaagtca caaaatctgc gcataataat
    15421 atcaaaacga cgtcgaattg atagtcgttc tcattactat ttgcatactg ccgtaccttt
    15481 gctttctttt ccttgcgttt acgcagtaaa aaagtcacca gcacgccatt tgcgaaaatt
    15541 ttctgcttta tgccaattct tcaggatgcg cccgcgaata ttcatgctag tttagacatc
    15601 cagacgtata aaaacaggaa tcccgacatg gcggacaaaa agcttgatac tcaactggtg
    15661 aatgcaggac gcagcaaaaa atacactctc ggcgcggtaa atagcgtgat tcagcgcgct
    15721 tcttcgctgg tctttgacag tgtggaagcc aaaaagcacg cgacgcgcaa tcgcgccaat
    15781 ggcgagttgt tctatggacg gcgcggaacg ttaacccatt tctccttaca acaagcgatg
    15841 tgtgaactgg aaggtggcgc aggctgcgtg ctatttccct gcggggcggc agcggttgct
    15901 aattccattc ttgcttttgt cgaacagggc gatcatgtgt tgatgaccaa caccgcctat
    15961 gaaccgagtc aggatttctg tagcaaaatc ctcagcaaac tgggcgtaac gacatcgtgg
    16021 tttgatccgc tgattggtgc cgatatcgtt aagcatctgc agccaaacac taaaatcgtg
    16081 tttctggaat cgccaggctc catcaccatg gaagtccacg acgttccggc gattgttgcc
    16141 gccgtgcgca gtgtggtccc ggatgccatc attatgatcg acaacacctg ggcagccggt
    16201 gtgctgttta aggcgctgga ttttggcatc gatgtttcca ttcaggccgc taccaaatat
    16261 ctggttgggc attcagatgc gatgattggc actgccgtgt gcaatgcccg ttgctgggag
    16321 cagctgcggg agaatgccta tctgatgggc cagatggtcg atgccgatac cgcctatata
    16381 accagccgtg gcctgcgcac attaggtgtg cgtttgcgtc aacatcatga aagcagtctg
    16441 aaagtggctg aatggctggc agaacatccg caagttgcgc gagttaacca ccctgctctg
    16501 cctggcagta aaggccacga attctggaaa cgagacttta caggcagcag cgggctattt
    16561 tcctttgtgc ttaagaaaaa actcagtaat gaagagctgg cgaactatct ggataacttc
    16621 agtttattca gcatggccta ctcgtggggc gggtatgaat cgttgatcct ggcaaatcaa
    16681 ccagaacata tcgccgccat tcgcccacaa ggcgagatcg attttagcgg gaccttgatt
    16741 cgcctgcata ttggtctgga agatgtcgac gatctgattg ccgatctgga cgccggtttt
    16801 gcgcggattg tataacattg ccacttttgg acaattttgc agacatttta ttgtgaaaag
    16861 tcttaaattg ttgcgtccgg gatcaaggcg tcccggacga ttcaggagta caataggcaa
    16921 ataaaggctt aaacgctgtt ccacaggaaa gtccatggct gttattcaag atatcatcgc
    16981 tgcgctctgg caacacgact ttgccgcgct ggcggatcct catattgtta gcgttgttta
    17041 ctttgtcatg tttgccacgc tgtttttaga aaacggcctg ctgcccgcct catttttgcc
    17101 aggcgacagc ttgttgatac tggcaggcgc attgattgcc cagggggtta tggattttct
    17161 gcctacgatt gcgattctga ccgccgcagc aagtctgggc tgctggctaa gttatattca
    17221 ggggcgctgg ttagggaata ccaaaacggt gaaaggctgg ctggcacagc ttcctgctaa
    17281 atatcaccag cgcgccacct gcatgtttga ccgccacggt ctgctggcgc tgctggctgg
    17341 acgttttctt gcatttgtcc gtacgctgct gccaaccatg gcgggaattt ccggtctgcc
    17401 aaaccgccgt ttccagtttt tcaactggtt aagcggattg ctgtgggtca gcgtggtaac
    17461 cagtttcggc tatgccttaa gtatgattcc gttcgttaaa cgccatgaag atcaggtaat
    17521 gacgttcctg atgatcctgc caattgcctt gttaaccgct ggcttgttag gcacgctgtt
    17581 tgtggtgatt aaaaaaaaat actgtaacgc ctgacgattt tccctgttcc cggttgctgt
    17641 accgggaacg tatttaattc ccctgcatcg cccgcattct tgccgcatct tcccccggcg
    17701 tcacaccgaa gtaacgttta aactcacggc taaattgcga tgcgctttca tagccgacgc
    17761 gcatcgctgc tgcgctggcc ttcatgccgt catggatgat catcatccgc gccttatgca
    17821 gacggtaatt cttcaaatac tgcaacggcg aggtgctggt gacagactta aaattatggt
    17881 ggaacgccga tacgctcatg ttggcttctg ccgccagttg ctcgacgctc aggttttcgg
    17941 tgtatttatt ctcaatccgt ttcagcacgc ggctaatcag actgaagtga gtctggcgac
    18001 tgaccagcgc cagtaacgcg ccgccgcaag gtccggtcag cacgtagtac agaatttcgc
    18061 ggatgatctg tttgccgaga atacgcgcat ccagtggtcg ctccatcacg tcgagtaacc
    18121 gctccgccgc gcataaaatc tcttctgata acgtggcgga gttaatcccg ctggctgcca
    18181 tcgacggctg gaaatgctca tcttcgccaa tgtccatcaa cagttcctgt aactgcaaaa
    18241 tatcgacatt gagacgcaac cctgccagcg gcacctctga cgtggcatag gtttcgcact
    18301 caaacggcaa cggcaccgtc agcagcaggt attcattggc atcataacga aacacgcgtt
    18361 cattgatata accgatttta tgcccggaaa agagaattat gatgccaggc tcgtacatca
    18421 ccggtgtacg tgcgaaaggc gtctcgccat acaacaaacg cacatcgggc aacagttctg
    18481 acaaactatt ttctttaatt ttcagtttat taactttatc cgccagcaag cggcaaatct
    18541 cttcacgttt catatcgcgt aatttcttag gaataatgcg gcaatttgat tgtgcgcaat
    18601 tttgtagcat ttctccagca ctctggagga ataggcaaga cattggcaga aatgagcatt
    18661 gagagccagg gcgctggcga tcacaatgaa aaacatcagg cagatcgttc tctgccctca
    18721 tattggccca gcaaagggag caagtaatga acaactttaa tctgcacacc ccaacccgca
    18781 ttctgtttgg taaaggcgca atcgctggtt tacgcgaaca aattcctcac gatgctcgcg
    18841 tattgattac ctacggtggc ggcagcgtga aaaaaaccgg cgttctcgat caagttctgg
    18901 atgccctgaa aggcatggac gtgctggaat ttggcggtat tgagccaaac ccggcttatg
    18961 aaacgctgat gaacgccgtg aaactgattc gcgaacagaa agtgactttc ctgctggcgg
    19021 ttggcggcgg ttctgtactg gacggcacca aatttatcgc cgcagcggct aactatccgg
    19081 aaaatatcga tccgtggcac attctgcaaa cgggcggtaa agagattaaa agcgccatcc
    19141 cgatgggctg tgtgctgacg ctgccagcaa ccggttcaga atccaacgca ggcgcggtga
    19201 tctcccgtaa aaccacaggc gacaagcagg cgttccattc tgcccatgtt cagccggtat
    19261 ttgccgtgct cgatccggtt tatacctaca ccctgccgcc gcgtcaggtg gctaacggcg
    19321 tagtggacgc ctttgtacac accgtggaac agtatgttac caaaccggtt gatgccaaaa
    19381 ttcaggaccg tttcgcagaa ggcattttgc tgacgctaat cgaagatggt ccgaaagccc
    19441 tgaaagagcc agaaaactac gatgtgcgcg ccaacgtcat gtgggcggcg actcaggcgc
    19501 tgaacggttt gattggcgct ggcgtaccgc aggactgggc aacgcatatg ctgggccacg
    19561 aactgactgc gatgcacggt ctggatcacg cgcaaacact ggctatcgtc ctgcctgcac
    19621 tgtggaatga aaaacgcgag accaagcgcg ctaagctgct gcaatatgct gaacgcgtct
    19681 ggaacatcac tgaaggttcc gacgatgagc gtattgacgc cgcgattgcc gcaacccgca
    19741 atttctttga gcaattaggc gtgccgaccc acctctccga ctacggtctg gacggcagct
    19801 ccatcccggc tttgctgaaa aaactggaag agcacggcat gacccaactg ggcgaaaatc
    19861 atgacattac gctggatgtc agccgccgta tatacgaagc cgcccgctaa gctttttacg
    19921 cctcaaactt tcgttttcgg gcatttcgtc cagacttaag ttcacaacac ctcaccggag
    19981 cctgctccgg tgagttcata taaaggagga acgtatggct aatccaaccg ttattaagct
    20041 acaggatggc aatgtcatgc cccagttggg actgggcgtc tggcaagcaa gtaatgagga
    20101 agtaatcacc gccattcaaa aagcgttaga agtgggttat cgctcgattg ataccgccgc
    20161 ggcctacaag aacgaagaag gtgtcggcaa agccctgaaa aatgcctcag tcaacagaga
    20221 agaactgttc atcaccacta agctgtggaa cgacgaccac aagcgccccc gcgaagccct
    20281 gctcgacagc ctgaaaaaac tccagcttga ttatatcgac ctctacttaa tgcactggcc
    20341 cgttcccgct atcgaccatt atgtcgaagc atggaaaggc atgatcgaat tgcaaaaaga
    20401 gggattaatc aaaagcatcg gcgtgtgcaa cttccagatc catcacctgc aacgcctgat
    20461 tgatgaaact ggcgtgacgc ctgtgataaa ccagatcgaa cttcatccgc tgatgcaaca
    20521 acgccagcta cacgcctgga acgcgacaca caaaatccag accgaatcct ggagcccatt
    20581 agcgcaagga gggaaaggcg ttttcgatca gaaagtcatt cgcgatctgg cagataaata
    20641 cggcaaaacc ccggcgcaga ttgttatccg ctggcatctg gatagcggcc tggtggtgat
    20701 cccgaaatcg gtcacacctt cacgtattgc cgaaaacttt gatgtctggg atttccgtct
    20761 cgacaaagac gaactcggcg aaattgcaaa actcgatcag ggcaagcgtc tcggtcccga
    20821 tcctgaccag ttcggcggct aacatgcaaa ttctcccggt ggcggtaatg ttccgctacc
    20881 ggacttttca gaaatcattt attcccctcg cgtcccgccc gttgttactc ttccttgttc
    20941 aggaatgcca aatataagga catcatcatg cagagccgga agctcttaaa agaacaactc
    21001 atctatatcc gggataaacg caacggagag gtgaaaaaca gatgaaaata atacttctgt
    21061 ttttagcagc cctggcaagt tttaccgtac acgcacagcc cccctcactg accgtagaac
    21121 aaacagtccg gcatatttat cagaactata aatcagatgc cactgcccct tattttggtg
    21181 aaaccggaga gcgggcgata acttctgcgc gtattcaaca ggcgcttacc ctgaacgaca
    21241 atcttacgct gccgggcaat attggctggc tggattatga tccggtttgt gattgtcagg
    21301 attttggcga tctggtgcta gaaagcgtag cgataactca aactgacgcc gatcatgccg
    21361 atgccgttgt gcgctttcgt atctttaaag atgataaaga aaagaccacg cagacactga
    21421 aaatggtggc ggaaaatggt cgttgggtca ttgacgatat tgtcagcaat catggcagcg
    21481 tcttacaagc agttaatagc gagaatgaaa aaacgctggc cgctttagct tcgttgcaaa
    21541 aagaacagcc ggaagccttt gttgccgaac tctttgaaca tattgctgat tatagctggc
    21601 cgtggacgtg ggtggtttcc gactcttacc gccaggcggt taatgccttc tataaaacca
    21661 ccttcaagac ggccaataat cccgatgaag atatgcaaat agaacggcaa tttatttacg
    21721 acaatccgat ctgttttggc gaagagtcgc tattttcacg cgttgatgaa attcgagtcc
    21781 tggagaaaac cgccgattcc gcccgcattc atgttcgttt tacgctgacc aatggcaaca
    21841 acgaagagca agaactggtt ttacagcggc gcgaaggcaa gtgggaaatc gctgatttta
    21901 tccgcccgaa cagcggcagc ctacttaagc agattgaggc aaaaaccgcc gccagattaa
    21961 agcaatgagc tgaattaaat aacaattagc cggaacaata aataaaaggg aacactatat
    22021 gaaaacgatt ttcaccgtgg gagttgttgt tctggcaacc tgcttgctca gtggctgcgt
    22081 caatgagcaa aaggtcaatc agctggcgag caatgtgcaa acattaaatg ccaaaatcgc
    22141 ccggcttgag caggatatga aagcactacg cccacaaatc tatgctgcca aatccgaagc
    22201 taacagagcc aatacgcgtc ttgatgctca ggactatttt gattgcctgc gctgcttgcg
    22261 tatgtacgca gaatgataaa aaaatccccg gcagcatgtc agttgccggg gatttttttt
    22321 aacgtccaac cgccgcttta gggcgtttct tcgcaccagc attcaccgga cgagattgcg
    22381 tagacgacgc tttttttgcc gtagcaggcg tctgacgctg ggtcgccatc ggcgtatgtt
    22441 tcgtcaacgc cggacgggta ttgcggttct ggcgacgagc ttcacgcatc tcttcaatgg
    22501 ttggcgcagg cactaagcaa tcgcgacggc tgccaatcag atgctttttg cccatcgctt
    22561 ccagcgcctg gcggattaac ggccagtttg ccggatcgtg gtaacgcaac aacgctttat
    22621 gcaaacgacg ctgtttgtcg cccttcggta cgaagacgtc ttcactctta taaccaatct
    22681 tcgccagcgg gtttttcccg gtgtaataca tggtggttga gttcgccagc ggcgatgggt
    22741 agaagttctg tacctggtcg agacggaaac gatgcttttt cagccacagc gccagattca
    22801 ccatatcttc atcacgcgta ccggggtgcg cggagatgaa ataggggatc agatactgct
    22861 ctttacctgc ctgttttgag taagtatcga acagctcttt aaagcggtca tagctgccca
    22921 tgcccggctt catcatcttc gataacggcc cttcttcggt atgttccggg gcaatcttca
    22981 gataaccgcc gacgtgatgg gtcgccaact ctttgatata gcgcggatct tccacggcga
    23041 tgtcataacg tacgcctgag gcgatgagga tttttttaat gcctttcaga tcacgcgcac
    23101 ggcgatagag gttgatcgtc ggttcgtggt tagtgtccat gtgcggacaa atatccggat
    23161 aaacgcacga caaacggcga caagtttgtt cagcgcgtgg cgatttgcag cgcaacatat
    23221 acatgttggc agttggccca ccgagatcgg aaattacgcc cgtaaaacca ggaacggtgt
    23281 cgcggatcgc ttcgatctca ttaatgatcg aatcttccga acggctctga ataatgcgcc
    23341 cttcgtgctc ggtgatagaa cagaaagagc agccgccaaa gcagccgcgc ataatgttga
    23401 ccgagaaacg gatcatttcg taagccggaa tacgggcatt gccataggcc ggatgcggca
    23461 cgcgcttgta cggcagcgca aaaacgctgt ccatctcttc ggtagaaagc gggatagcag
    23521 gcgggttgat ccacacatag cggtcaccgt gtttttgcat caatgcgcgg gcgcagcctg
    23581 ggttggtttc gtggtgcaga atgcgcgaag catgagcgta caggacttta tcgcccttca
    23641 ctttctcgaa agaaggcagc aatacgtagg ttttttccca cggtttcggg cgcggcggct
    23701 gcacggttac agctttcgct tcctgctttt tcggcgcaac aggtttgtta tccgcgcacg
    23761 gcagatcttc gccatacgga tgcgggattg ggtcgatttt ccctggggta tcaagacgag
    23821 tagaatccac accgctccag ccaggcagcg cctcttttac gataattgcg gtattacgca
    23881 catcgcggat ttcgctaatt ggctcgccca tcgccagacg atgcgctact tccaccagcg
    23941 gacgttcacc gttaccaaac atcagcatgt cggctttcga atccaccagc acggaacggc
    24001 gcacggtatc ggaccagtaa tcataatgcg cggtacggcg cagactagcc tcaataccgc
    24061 caaggatcac cggcacatct ttccacgcct ctttacaacg ctgggtataa accagtgtgg
    24121 cgcgatccgg tcgcttaccc gcgacgttat ccggtgtgta ggcatcgtca tgacgtaaac
    24181 ggcgatcggc ggtatagcgg ttgatcatcg aatccatgtt gccagcagta acaccaaaaa
    24241 acagattcgg tttacccaga cgcataaagt cgtctttgct gctccagtcc ggctgggcga
    24301 tgatcccgac gcgaaagccc tgcgcttcca gcatacgacc gcaaatcgcc atcccgaagc
    24361 ttgggtgatc gacatacgcg tcgccagtaa ccaaaatgat gtcgcagcta tcccagccaa
    24421 gttgatccat ctcttcacga gacatcggca aaaacggtgc cggtccaaaa caggcggccc
    24481 agtactgcgg ccaggagaac aggtcgcgat ccggttggat cagggagata gagctcattt
    24541 tgcttccaga aatgataaaa aaataatcaa aggccgggga ttataagccg gaacgaaaga
    24601 gaaatcgaaa ggtattccat actcgccctc ctcgggcgag tatgaagatt acggtaccgg
    24661 attgaccaac agttgcccaa tcgagccacg gtccgccatt tccagcgtct gactgttgaa
    24721 gtagaacggg aagtgcgccc aggaaggctg accgaaataa acaagcagct ccacttgtcc
    24781 gtcaacccaa acggtatctt tccagcctct gtcttccggg aacggcatcg cgccattcac
    24841 gttacggatc tggaacatca cgccttcaat atggaacgct tgcggctcgt ccgcgcgtac
    24901 cgtccagcgt tcccacgttc cttgctgcgc ggtgacatca atacggttga cgtcccacag
    24961 ctgtccatta atacccgggt catcacccag actaatatcg cgactgcgaa tcggcgaacc
    25021 ggccatgatt tcagttggca gcaagcgcat cggaagactg tctgtgacca gcggcagaag
    25081 gccggttggg cgcagcgtta gcaccagggt agaaaccaaa atgctggatg gctcaaagaa
    25141 gccacgaata cgatcaacaa tgctcgccgc ttcgccacag gtgatcgaca cttcatcacc
    25201 gttgctcata tccaccagaa tctcgcggcg ctcgcccggt gccagcgaaa gttgcttcac
    25261 cgatacagga gcaggcagga atccctgatc gccagaaatc acatgtaacg ggcgaccatc
    25321 gctcatctgt agttgatagc gacgagagtt cgacgcgttc agcagtcgca agcgcaccca
    25381 accacgcgag acttcaacgt acgggctttg tacaccgtta accagcagcg tatcaccaac
    25441 aaagccgccg cttcccggtt cgttgtattc tggcgtacca aagttatcca gccgtttatc
    25501 ctggataatg accggaaaat catccacacc ataatggttg gggataggca gcgacttgct
    25561 gacttcatct tccaccagcc acattccggc aaggccgtta tagacctgct gagccgtgcg
    25621 gttgggagta ttggcgtgat accacagagt agctgcgttc tggcgaatgg gcagtacggg
    25681 tgcccagtca gcgtttggcg acatcatccg tgccggaccg cccatcagcg ggcctggtac
    25741 ctgtagcccg gccaccgtca ttgagacatt ttctgtcagg cggttgctgt aaataagctt
    25801 aacatcgtcg cccttccaga cgcggatagt cggccccagg taacgaccat tgattcccca
    25861 gaccgacgcg cgtgtccctg gcgtaaatga ccagtgcgca cgttgtacag tcataaacag
    25921 cggttgccca cggcgagatt caagtagcgg cggaacgggt agcggttgct gttgcccggc
    25981 tgcgctggcc ttcaggggaa cagcgcctgc acaaagtgca atccccgatg cctgaatgaa
    26041 ctgacgccga ctgagtgaca tatttgctcc atgtaaaact ggctaaagta ataccgtgca
    26101 ggaaagtgtt ccccgcaaaa atacagattg ccgacttaaa cttttccggc ggcttcgcgt
    26161 tctgcgactt ctttatcgag ctcggcgatt ttttgttcca ttatcgaacg acaatgggca
    26221 gccagctcac gaacctgatc tttgccatac tgactgacgt caattggcgg cagcatttcg
    26281 acaatcacca gaccgttgtg cagtcgatta agattaatct tattcgaagt tgtagagacg
    26341 cacacgggaa taatcgggac gcccgccgca attgccgcgt gaaatgctcc agtcttgaac
    26401 ggtagcaggc cgcgaccacg gctgcgggtt ccttccggga acatccagat ggaaatacgg
    26461 cgttttttga aatgattcac gacttccgca atggtgccgt gagctttagt gcgattgttt
    26521 ctgtcgatca ataagttgcc ggttaaccag tacaactgcc cgaagaaggg gatccacagc
    26581 aagctctttt tacctaccgt caccgtcggc ggttgcacaa tgttcgatgc tgtcaccatg
    26641 tcatagttgt tctggtggtt agcgatatag atagcattgc cgtagctttc agcatctgca
    26701 ggtttgcggc actcaacttt caggccgaac agcggcgcaa gacggccaaa catatgccca
    26761 aaggtggcca catgtttcgg gttacgcggg ctgaaaaggc agtaaatgga gccgaataca
    26821 cagactaaga tgctgtaaat caaggtaata ataagacgaa agatatatag cataacaccc
    26881 tctgaaggtc tgagagactg gcattgtacg tcacctgaaa tcaggttagg gcattgttaa
    26941 aagccgctct ctggaaaagc gtattgttaa tcgcaacgca tgaataaaca acggtttcaa
    27001 gggaattttt atcgtttccg ggggttgaat acgctgccgg atgacgactt aacgtttcat
    27061 ccggcgttcc ttgcaagcgg gaggaaacag gccctccccg gcatattact cttcgctatc
    27121 accgctgctg gcacggcgag gagagtcgat ctcaacacga tcgatacgct gcaaaccgcg
    27181 catcaacgta ccgcggcgtc cacgttcgcc agtgactttc tgtaactctt ccgggcgcag
    27241 tttaattttg cgtttcccaa catgaatggt cagcgtgctt tgcggcggca gaacgtacaa
    27301 ttgcgccaga ccatcttctc cacgcgcggc ttctgccgat ggaatgttga taatcttgtt
    27361 gcctttgccc ttcgacagct gcggcagatc gcttaccggg aacatcaaca tacggcctgc
    27421 ctgagtgatt gccagcagca tatcggaagc atcttcaatc accaccggcg gcataacatg
    27481 ggcattttcc ggtaaggtga tcaaagcctt acctgcacgg ttacgcgcca ccagatcgtt
    27541 aaaggtgcag acgaaaccgt aacccgcatc ggaagccatc agcagtttct gatcgtcgct
    27601 ttccatcagc atatggtcaa cggtcgcccc aggcggcaac gttaatttgc cggtgagtgg
    27661 ttcgccctga ccacgcgccg acggcagcgt aatcgggtcg atggcatagc tacgaccggt
    27721 ggaatcaaca aacactaccg gttggttgct cttacctttc accgccgctt tgaagctatc
    27781 acccgcttta taattcaggc ccggcgcgtc gatatcatgg cctttagcgc tgcgtaccca
    27841 gcccatctgc gacagcacaa tggtgacagg ttcagacggc agcatgtcgt gctcgctcat
    27901 cgctttcgct tcttcgcgtt cctgcaacgg cgaacgacga tcgtcaccgt aggcttgcgc
    27961 gtctgcctgc agttctttct tcagcaggtt attcatttta cgctcggaag ccaaaatgcc
    28021 ctgcaactgg tcgcgctctt tttccagctc actctgctca ccgcgaatct tcatctcttc
    28081 cagtttggca agatgacgca gtttcagttc gaggatcgct tccgcctggg tttccgtaag
    28141 gccaaaccgc gacatcagcg ccggtttcgg ttcatcttcg ttacgaatga tctcaatcac
    28201 ttcgtcgatg ttgagaaacg ccaccagcaa accttccagg atatgcaggc gcttgaggac
    28261 tttctccaga cgatagttca gtcggcggcg cacggtatcg cgacggaaca ccagccattc
    28321 ggagaggatt tccagcaggt ttttcaccgc cggacgacca tccagaccga tcatattgag
    28381 gttaatgcga tagctctttt ccagatcggt ggtagcgaag aggtggttca tcacctgatc
    28441 catatccacg cggttggaac gcggcacaat caccagacgg gtcgggttct cgtggtcaga
    28501 ttcatcgcgc agatcgtcaa ccatcggcag ctttttgttg cgcatttgcg cagcaatttg
    28561 ctccagtacg cgcgcacctg aaacctgatg cggtaatgcg ctgataacca ccgcgccatc
    28621 ttctttcttc cacaccgcgc gcatacgcac tgaaccacgt ccgttctcgt agattttacg
    28681 gatctcggcg cgcgaagtga taatttccgc ttcagtcgga taatccggcc cctgcacgat
    28741 atccagcagc tgatcgagcg tggttttcgg ctggtcgatt aatgcgattg ccgcctgagc
    28801 tacttcacgc aggttatgcg gtggaatatc ggtcgccatg ccgacggcaa taccggtggt
    28861 gccgttaagc aaaatgtttg gcagacgagc aggcagcatt ttcggctcct gcaaagtgcc
    28921 gtcgaagttt ggcacccagt cagccgtccc ctgccccagc tcgctcaata gcagctcgga
    28981 atatttcgac aaccgggatt cggtgtaacg cattgccgcg aacgatttcg gatcgtccgg
    29041 cgcgccccag ttcccctgac catcaaccag cggataacgg taagagaacg gctgcgccat
    29101 caggaccatc gcttcataac aggcgctatc gccgtgcgga tggtatttac ccagtacgtc
    29161 accgacggta cgggccgatt ttttaaattt ggcgctggca ttcaggccca gttcagacat
    29221 cgcatacaca atgcggcgct gaacaggttt aagaccatcg ccaataaacg gcaacgcacg
    29281 gtccatgatc acgtacatgg agtagtttaa gtaggcgttt tccgtaaatt catgtagcgc
    29341 aaggcgctct gccatatcgc tcattaattc tgattcctca acttattcgc cagccttcaa
    29401 aggggcaata ttgccggaga tactacatta tctgccgcgt cgagtcacag gagatcagcg
    29461 tgaatagcgc cgccggacgt gctccgacgg cgataaaggc atcaatgtgc cttgatatac
    29521 aactctttgc tgtagacttg atcggcatag agggcagcac aaggttgcgc ccatgccaca
    29581 ccactcggca tgggggtccg ctccgggcga ttcgagaagt tgaggttatg cgagtccagg
    29641 gaagcctaaa gcatcaaagt agtcgactgt aagtatacgg ttcagcccag tttagctgtg
    29701 tctccaccag tgccccgcgc ctcctgccat aagtgcggct tgctcatgga tacccccgag
    29761 acggcttacg gcgcaccgga taataaattt tgcgtataca tggtattcct taccggagaa
    29821 attataagcc caacaacaat acgcctgtgg cattgttcgc tcaaacttat cgcgagtgat
    29881 ttggctcaca ctccggtaaa gattccccat cacgccaacg gcacataaac atccgtttcc
    29941 agtcgctgct ctgtcacccc ttcggcaaga ttggtgtagt gaaacagaat cggtgcttta
    30001 cgcattttct cgccgcttgc aggcagccag tggcgaataa tgccccatat cgtgtgtgaa
    30061 atatcgtcca gctcgccaac gtggcgggcc acggcataac gtccaccggt aagttcacca
    30121 ttgctcacac cataacgatt atcgggaatc ggttcgctaa cgctgccgca gatatcaaag
    30181 cgaaacgctt ccggtgcggt ggtggcaggg tcgtcccagg cgacgccaaa agtctggctt
    30241 tggttaacag gagaaagccc cgtctcttta cgccacatga taaatttcgc cacactgtag
    30301 ttgagcaact cagggctaca gcggtgcggc aacatcgcca cagggattga tgggaaatcg
    30361 ataatgttta catccagtgt caggtttgtc attttgcctc cctgcgaaat tattcccggg
    30421 aacgcgtccc gggagcggta acaattacgg atttactttg cggatctgtt tgacgtcaat
    30481 ttcaacagaa ttccagtctt tatcgacttc accctgaatc tcaaccgtat ctttcggcgt
    30541 caccgtcaca ccgttccagc gtttgtggtc gatatcaaca ttgatagtac cgctggcatc
    30601 tttaaacacg tagagatcgt cagagatgcg ttcaacgata ttgccgcgca gggttaccca
    30661 ggtgtcgtca cgcagggatt ttgcgctttc tacggtcgtt acgctgccgt tcggcccctg
    30721 gaatcctccg gcctgacttt gcgttgccga tgggccagaa aaaccgccct gctctgctgc
    30781 catcaccggt gcgctgcaca gggccattac tgcgattact gctgcgaatt ttttcatgtt
    30841 tattactccc tttaatgtct gtttccgagc atttaacaag atagtcctta acaacttctt
    30901 aagggaaaaa aataaaattt agtgctgtac agagcgcgtt acaacacggt ttactggcag
    30961 caaatacggt tatcgcaggg atgaaaaaat gcgaatttta ctgatagaag atgacatgct
    31021 gattggcgac ggcatcaaaa cgggccttag taaaatgggt tttagcgtcg actggtttac
    31081 acaaggtcgt cagggaaaag aggcgcttta tagcgcgcct tatgatgcgg tgatcctgga
    31141 tttaacctta ccaggcatgg atggtcgcga tattttgcgc gaatggcgag aaaaaggtca
    31201 gcgtgagccg gtactgatcc tgaccgcgcg cgatgcgctg gaggaacgtg tagaagggct
    31261 gcgtctggga gctgacgatt atctgtgtaa accttttgcg ttgatagaag tcgccgccag
    31321 gctggaagct ctgatgcgcc gaaccaacgg ccaggccagc aacgagctgc gccacggtaa
    31381 cgtcatgctc gaccccggca aacgtatcgc cacgctggct ggcgaaccct taacactgaa
    31441 accaaaagaa tttgccctgc tggaattact gatgcgtaac gctggtcggg tactgccgcg
    31501 caaactgatt gaagagaaac tgtatacctg ggacgaagag gtcaccagta atgccgttga
    31561 agtgcatgtg catcatctgc gacgcaaact cggtagtgat tttattcgta ccgtgcatgg
    31621 tattggctac acattaggtg agaaatgaaa tttacccaac gtcttagtct gcgcgtcagg
    31681 ctgacgctaa tctttttaat tctggcctcg gtgacctggc tgctttccag ctttgtcgcc
    31741 tggaaacaaa caacggataa cgtcgatgaa ttgttcgaca cccaactgat gctgtttgcc
    31801 aagcggttaa gtacgctcga tctcaacgaa atcaacgcgg cggatcgcat ggcacagacg
    31861 ccaaataaat taaaacacgg tcatgttgat gacgatgcgc tgacctttgc catctttacc
    31921 cacgacggca gaatggtcct taacgatggc gataacggag aagatattcc ttatagctat
    31981 caacgggaag gttttgctga cgggcaactg gtgggcgata aagatcaatg gcgttttgtc
    32041 tggatgacct cacctgatgg caaatatcgc atcgttgttg gtcaggaatg ggaataccgt
    32101 gaagacatgg cgctggcgat tgttgccggg caattgatcc cgtggctggt cgcactgccg
    32161 attatgttaa tcatcatgat ggtactattg ggtcgtgaac tcgcgccgct gaacaaactg
    32221 gcgctggcgc tgcgtatgcg tgaccctgac tcggaaaaac cactaaacgc aactggcgta
    32281 cccagcgaag tgcgtccgtt ggttgagtcg ctaaatcaac tgttcgcccg cacacatgcg
    32341 atgatggttc gtgaacgacg ctttacctcc gacgcagctc acgaactacg tagcccgtta
    32401 acggcgctga aagtgcaaac cgaagttgcg cagctttctg acgatgatcc gcaagcgcgg
    32461 aaaaaagcgc tgcttcaatt acattacggg atcgatcgcg ctactcgtct ggttgatcaa
    32521 ctgctcactc tatcgcggct ggactcactg gataaccttc aggacgtcgc ggagatcccg
    32581 cttgaagatc tcctgcaatc gtcggtgatg gatatttacc acacggcgca gcaggcgaaa
    32641 attgacgtgc gactgacact caatgcccac ggcatcaaac gcaccggaca accgctattg
    32701 ctaagtttgt tggtgcgaaa tttgctggat aacgccgtgc gctacagtcc acagggcagc
    32761 gtgatagacg tcacgctgaa tgctgataat ttcatcgtga gggataacgg ccccggtgtg
    32821 acgccagagg ctctggcgcg aattggcgaa cgcttctatc gcccccccgg acaaaccgct
    32881 accggcagtg gacttgggct atcgattgtc cagcgaatcg ccaaattgca tggcatgaat
    32941 gttgagtttg ggaatgcgga acaaggtgga tttgaggcga aggtaagctg gtaacttctc
    33001 tgtctatgag agccgttaaa acgactctca tagattttac taatagcaaa atataaaccg
    33061 tccccaaaaa agccaccaac cacaaaccag acaaataccg agcgaaaata catataccca
    33121 aaactcgaac atttcccgca taaagagttt tcttaagata agaataataa gtggcgtaag
    33181 aagaaaaaat gctgcaatca ttgcttgcaa tatacgatct tcgcctgaaa cggaaggtat
    33241 actgcaaata ttgtctcccg actcaagcat ccagtcatac ttattgaaag caaaaaaagc
    33301 atctattaac caataagtaa gcattatgta taacagagct tcaaatattg tttgaatttt
    33361 atgctttaac atatatcagt acgctcataa aaatccattt ataattatca acatgacacg
    33421 ttgatatttt catcttttac ttattcaaag ccatattttc aatcccaaaa aaaaccgaac
    33481 caaacgtgta aattattccg gtgcgagtaa gaattatgag gaatggctat cagtattgtc
    33541 attttcagaa aatatttatc ctgcatcggt gagtcagagc aagatcagac ttttgctaaa
    33601 ttcgcaaaag actttgcaca ttttgctaat ttcaccgtac cgctctgtga cgtactatag
    33661 tcggcaaacg tctcaccttg aggttaaaaa atgagcaaca tcctgattat caacggcgcg
    33721 aaaaaattcg cccactccaa tggtcaactg aacgacaccc tgaccgaagt cgctgatggc
    33781 acactgcgcg acctcgggca tgatgtccgc atcgttcgcg ccgacagcga ctacgatgtc
    33841 aaagcggaag tacaaaactt tctctgggct gatgtggtga tctggcagat gccaggctgg
    33901 tggatgggcg cgccgtggac agtgaaaaaa tatattgatg atgtattcac cgaaggtcat
    33961 ggaacgctgt atgccagcga tggccgtacc cgcaaagatc cgtcaaaaaa atacggttcc
    34021 ggcggcctgg tacagggcaa aaaatatatg ctttctctga cctggaacgc accaatggaa
    34081 gccttcaccg aaaaagatca attcttccac ggcgttggcg tcgacggtgt gtatctgccg
    34141 ttccataaag caaaccaatt ccttggcatg gaaccgctgc cgacatttat cgctaatgac
    34201 gtgataaaaa tgcctgatgt tccccgctat actgaagaat atcgcaagca tcttgtggaa
    34261 atttttggtt aactagagct caggctttag aaggagttaa ccatgcttac cgtaatcgca
    34321 gaaatccgta ctcgtcctgg tcaacatcac cgtcaggcgg tattggatca gtttgctaaa
    34381 atcgttccaa ccgtactgaa agaagaaggt tgccacggct atgcgccaat ggtggattgc
    34441 gctgctggcg tgagtttcca gtctatggca ccggattcta tcgtgatgat tgagcagtgg
    34501 gaaagcatcg cgcatcttga agcgcatctg caaaccccgc acatgaaggc gtacagcgaa
    34561 gccgtaaaag gcgacgtgct ggagatgaat atccgcatcc tgcaacctgg gatttaaacc
    34621 tgccttgttt gcccggccat cctgaccggg caatgttctt tcctttaaac ctcaatctcc
    34681 gccatgtcgc ctttctcttg caaccagttg cggcgatctt ccgagcgttt cttcgccagc
    34741 agcatatcca tcatcgcgtc agtacgctga tcgtcttcat catcgatagt caactgcacc
    34801 agacggcgag tgttcggatc aagcgtggtt tcacgcaatt gcatcgggtt catttccccc
    34861 agacctttaa aacgctggac gttcggcttg cctttcttgc gttttaattg ctcaagtacg
    34921 ccctctttct cttcttccgt cagcgcgtaa taaacctctt tcccgagatc aatacggtag
    34981 agcggtggca gtgcgacgta aacgtgaccg tgtttcacca acgcgcggaa gtgttttacg
    35041 aacaaagcgc agagcagcgt ggcaatgtgc agaccatcag agtccgcatc cgcgaggata
    35101 cagattttgc cataacgaag ctggctcaga tcgtcgctgt caggatcgat accgatcgct
    35161 accgaaatat catgcacttc ctgcgaggcc agcacttcgt cggaagaaac ttcccaggtg
    35221 ttaaggatct tacctttcag tggcatgatc gcctgatatt cgcgatcgcg cgcctgcttg
    35281 gcagatccgc ctgcggagtc accttccaca aggaacagtt cggtacggtt aagatcctgc
    35341 gcggtacagt cagccagttt gccaggcagt gctgggccgc tggtcagctt tttgcgcacc
    35401 acttttttag ccgcacgcat acggcgctgg gcgctggaaa tcgccatctc cgccagcagc
    35461 tccgccgcct gaacgttctg gttcagccac aggataaagg catctttcac cacgcctgaa
    35521 acgaatgccg cgcattgacg cgaagagaga cgctctttgg tctgcccggc aaactgcgga
    35581 tcctgcattt tcaccgacag aacataggcg cagcgatccc agatatcttc cgccgacagc
    35641 ttcacgccgc gcggcagaat gttgcggtat tcacagaact cacgcatcgc gtccaacagg
    35701 ccctgacgca gaccattaac atgggtaccg ccctgcatcg ttgggataag gttgacgtag
    35761 ctttcggtca gcagttcacc gccttccggc agccacagta gcgcccagtc cacagcttca
    35821 gtatcgccag cgaaattacc gataaacggt ttttctggca gcgtcggtaa accgtttacc
    35881 gcttccgcca ggtaatcatt cagaccgtcc tgatagcacc agcgctgttc ggtgttgttg
    35941 atctcatctt taaaggtaat ctcaacgccg gggcacagta ctgctttggc cttcagcaca
    36001 tgcgtcaggc gtgaaacaga aaaacgcggg ctgtcaaaga aggtttcatc cggccagaag
    36061 tgcacgctgg taccggtatt gcgtttaccg caagtgccga caacctgtaa atcctgcacc
    36121 ttttcgccat tttcaaaggc gatgttataa acctgaccat cgcggcgcac gttaacttct
    36181 acgcgcttcg acagggcgtt aaccaccgaa atccccacgc catgcaggcc gccagagaac
    36241 tggtaatttt tgttagagaa tttaccgcct gcatgcagac ggcaaagaat cagttcaacc
    36301 gccggtacac cctcttccgg gtgaatatcc accggcatcc cgcgcccatc gtcaataact
    36361 tctaacgact ggtcagcatg taagattaca tccacgcgtt ttgcgtgacc cgccagtgct
    36421 tcatcgacac tgttatcaat aacttcttgc cccaaatggt tagggcgagt ggtatcggta
    36481 tacatccccg gacggcggcg aaccggctca agcccggtga gtacctcaat ggcatcagcg
    36541 ttataagttt gcgtcatggt ttaagttagc aattcgagtt gatcgtcaga gatggtgcag
    36601 accaagaaaa tcgacgatcg ggttgaaata atcttcgaag cccgtgaatg cgtggttgcc
    36661 gccttctatg acagtctggc ggcaggaagc gtagtacgcc accgcctggc ggtaatccag
    36721 cacttcatct cccgtctgtt gcagcagcca gatcaaatcc ggcgcttcca gcgggtcaat
    36781 ctgcatgact ttgagatcgt aaatatggcg tgactctagc acatattgct gcccggtgta
    36841 ggggttctcg ttctgaccga gatagtccgt cagcagttca aacgggcgca ccgccgggtt
    36901 taccaccact gcgggcagca taaaacattg tgacaaccag gtggcgtaat atccccccag
    36961 tgacgaaccg acaataccca gcgaatcgcc gccatgttcc aggacaatgg attccagcag
    37021 ctctgccgcg tcggaaggat acggcggcaa ctgcggaatg atcatctcaa cgtcagggtg
    37081 atgttccgcc agccagtttt ttaacaagct cgcttttgca gagcgcggcg agctgttgaa
    37141 accgtgtaaa taaagaagcg tagacatcag tagccttctg aagcggtatc aggttggaaa
    37201 cgtgtgtccg ccaggcgatg cacctcggtg gtcagcgtgc catcagcatg taactcgaga
    37261 gtacgccagc cgggcgcgat agtatccagc gtaaagttgg aacagtgcgg cttaaactgc
    37321 acacaggtcg acggcgttgc cagcaggcgg cgaccgttcc agtcgagatc cagctcctga
    37381 tgaatatgac cgcacagcaa gtatttgacg tgcggaaact tcgccagcac ggtatccagt
    37441 tcgcccgcgt tacgcagact gtgttgatcg agccaactac aacccgcagg tagcggatga
    37501 tgatgcagca gcagcaacgt atgacgttct ggcgcatcgg ccagtttacg ttccagccac
    37561 tcaagctgaa actcgctcag ctcaccgtgc ggcacgccaa acacctggct atccagcaac
    37621 aggatttgcc attgctcacc aataaacacg cgcttcgccg gggagatacc cgcatcctgt
    37681 aacgcgctgt acatcgcggg ctggaaatcg tggttgcccg gcagccagac gcagggcgca
    37741 cgaaaacttg cgatgccttc agcgaaatgc tgataggccg cagaggattg atcctgcgct
    37801 aaatcacctg tcgcgacaat caggtcgaat tcgtgctgat gtggacgaat cgcctccagc
    37861 accgcctggt agctttccca ggtgtttacc cctaacaggg cttcgtgctt ttgtgcaaac
    37921 aggtgagtat cggtaatttg taaaatcctg actctggcct caccagccag aggaagggtt
    37981 aacaggcttt ccaaatggtg tccttaggtt tcacgacgct agtaaaccgg aatcgccatc
    38041 gctccatgtg ctaaacagta tcgcaaccag tccgctaaaa actggttaat ttgatgcttt
    38101 tcgtcgcgtt gatgcaactt tttattagga taatcatacc gcgctttgaa gcgaaaaatc
    38161 tgctggcttg aacacacttc agccaccatc gcgtcatgat acagacgcac cgtcattgac
    38221 ggaaggctcc agtaactgat cgcgggcgca gtctgttcta ttgtcaccag ggtagtgtat
    38281 cgggtcgatt ccacaatcgt cagccgatat tgtgcgtttg ccacctgata gcttacagtt
    38341 tcgccgggtg cgtcattgcg cggtaacaaa cggcgcaatt gtgaaaagtt catctcgcac
    38401 aggcgcatca tttcaggaaa gtcaggtgtg taacgcttca tttatgccca ctcatttttt
    38461 aacgcttgat gatgcagctg cagccattgc aaagcgatga ccgacgctgc gttgtcgatt
    38521 ttcccctctt ctacccactg gtatgcctgt tcccggctta ccacatgaac gcgaatatct
    38581 tcgttttcat cagccagacc atgaataccg cttgcggtcg tggcgtccac ttcgcccacc
    38641 ataattgagg aacgctcact ggtgcccccc gggcttgcca ggaaacttaa caccggtttg
    38701 gtccgtttga ctatcagtcc cgcctcttca atcgcttcgc gacgggcaac atcttccaca
    38761 ctttcaccct cttctatcat cccggcaacc atctccagta gccagggggt ttcactggtg
    38821 tcgtatgcgg caatccgaat ctgctcaatc agcacaactt catcacgcac tgggtcaaag
    38881 ggtagcaaga ctgcggcgtg accgcgctca aaaatttccc tccgtacctc atgactcatt
    38941 tgcccgttga ataaacgatg acgaaatcta taaagatcta atgaaaaaaa gccgcgataa
    39001 agtgtttctc gtgcaataat ttctacatcg tttttgccaa atgtaacggg caggttgtct
    39061 ggcttaagca ttgttaatgt cctggcacta atagtgaatt aaatgtgaat ttcagcgacg
    39121 tttgactgcc gtttgagcag tcatgtgtta aattgaggca cattaacgcc ctatggcacg
    39181 taacgccaac cttttgcggt agcggcttct gctagaatcc gcaataattt tacagtttga
    39241 tcgcgctaaa tactgcttca ccacaaggaa tgcaaatgaa gaaattgctc cccattctta
    39301 tcggcctgag cctttctggg ttcagttcgt tgagccaggc cgagaacctg atgcaagttt
    39361 atcagcaagc acgccttagt aacccggaat tgcgtaagtc tgccgccgat cgtgatgctg
    39421 cctttgaaaa aattaatgaa gcgcgcagtc cattactgcc acagctaggt ttaggtgcag
    39481 attacaccta tagcaacggc taccgcgacg cgaacggcat caactctaac gcgaccagtg
    39541 cgtccctgca gttaactcaa tccatttttg atatgtcgaa atggcgtgcg ttaacgctgc
    39601 aggaaaaagc agcagggatt caggacgtca catatcagac cgatcagcaa accttgatcc
    39661 tcaacaccgc gaccgcttat ttcaacgtgt tgaatgctat tgacgttctt tcctatacac
    39721 aggcacaaaa agaagcgatc taccgtcaat tagatcaaac cacccaacgt tttaacgtgg
    39781 gcctggtagc gataaccgac gtgcagaacg cccgcgcgca gtacgatacc gtgctggcga
    39841 acgaagtgac cgcacgtaat aaccttgata acgcggtaga gcagctgcgc cagatcaccg
    39901 gtaactacta tccagaactg gcagcgctga atgtcgaaaa ctttaaaacc gacaaaccac
    39961 agccggttaa cgcgctgctg aaagaagccg aaaaacgcaa cctgtcgctg ttacaggcac
    40021 gcttgagcca ggacctggcg cgcgagcaaa ttcgccaggc gcaggatggt cacttaccga
    40081 cgctggattt aacggcttct accgggattt ctgacacctc ttatagcggt tcgaaaactc
    40141 gtggtgccgc tggtacccag tatgacgaca gcaatatggg ccagaacaaa gtgggcctga
    40201 gcttctcgct gccgatttat cagggcggaa tggttaactc gcaggtgaaa caggcccagt
    40261 acaactttgt tggtgccagc gagcaactgg aaagcgcgca tcgtagcgtc gtgcaaaccg
    40321 tacgttcctc cttcaacaac attaatgcat ctatcagtag cattaacgcc tacaaacaag
    40381 ccgtagtttc cgctcaaagc tcattagacg cgatggaagc gggctactcg gtcggtacgc
    40441 gtaccattgt tgatgtgttg gatgcaacca ccacgctgta caacgctaag caagagctgg
    40501 caaatgcgcg ttataactac ctgattaatc agctgaatat taagtcagcc ctgggtacgt
    40561 tgaacgagca ggatctgctg gcactgaaca atgcgctgag caaaccggtt tccactaatc
    40621 cggaaaacgt tgccccgcaa acgccggaac agaatgctat tgctgatggt tatgcgcctg
    40681 atagcccggc acccgtcgtt cagcaaacat ccgcacgcac taccaccagt aacggtcata
    40741 accctttccg taactgatga cgacgacggg gcttcggccc cgtctgaacg taaggcaacg
    40801 taaagatacg ggttatctgc cgcattcttc ccccttctcg cttcaatttc gaccagccat
    40861 cctctattct gatgggtatt taccactggt cccggaagac aaaaatgaaa cggacaaaat
    40921 ccatacgcca cgcatcgttc cgcaaaaact ggagcgcgcg ccatctgaca ccagtcgctc
    40981 tcgcggttgc cactgttttt atgctggctg gctgtgaaaa gagtgatgaa acagtgtctc
    41041 tctatcaaaa tgctgacgac tgttcagctg caaacccagg caaaagcgcc gaatgtacca
    41101 ccgcgtacaa caatgcgctg aaagaagccg aacgtactgc gccgaaatac gccacccgtg
    41161 aagactgtgt tgctgaattt ggtgaaggtc agtgccagca ggcaccagcc caggctggca
    41221 tggcaccaga aaaccaggcg caggcccagc aatccagcgg gagtttctgg atgccgctga
    41281 tggccggtta catgatgggg cgtctgatgg gcggcggcgc gggatttgca cagcagccgc
    41341 tgttctcctc gaaaaaccca gccagcccgg cttacggtaa atataccgac gcgacgggta
    41401 aaaactatgg cgcagcccag ccaggccgca ccatgaccgt accgaagaca gccatggcac
    41461 caaaaccggc gaccaccact accgttaccc gtggcggttt tggtgaatct gttgccaaac
    41521 aaagcactat gcagcgtagc gcaactggca cctcttctcg ttcaatgggt ggctgatacc
    41581 gatggaaaga gtcagtatta ccgagcgccc ggactggcgt gagaaagccc acgaatacgg
    41641 tttcaatttt cacaccatgt acggcgagcc gtactggtgt gaagatgctt actacaagtt
    41701 gaccctcgcc caggttgaaa agctggaaga agtcaccgcc gaactgcacc agatgtgcct
    41761 gaaagtggtg gaaaaagtga tcgccagcga tgagctgatg accaaattcc gcattccaaa
    41821 acacacctgg agttttgtgc gccagtcatg gctgacgcac cagccatcgc tttattcgcg
    41881 tcttgatctg gcgtgggatg gcactggcga acctaaactt ctggaaaata acgccgatac
    41941 gccaacgtca ctatacgagg cggcgttctt tcagtggatc tggctggaag atcagcttaa
    42001 cgccggtaac ttgccggagg gcagcgacca gtttaacagt ctgcaagaaa aactgatcga
    42061 tcgcttcgtt gagctgcgtg aacagtatgg cttccagttg ctgcatctca cctgctgtcg
    42121 cgacacggtg gaagatcgcg gaaccattca gtatttgcag gactgcgcaa cggaagctga
    42181 aattgctact gagttcctct acatcgatga tatcgggtta ggtgaaaaag gtcagttcac
    42241 ggatttacag gatcaggtaa tttccaacct gttcaagctg tatccgtggg aatttatgtt
    42301 gcgtgagatg ttttccacca agctggagga tgcaggcgta cgctggctgg aaccggcgtg
    42361 gaagagcatt atctccaaca aggcacttct accgctactg tgggagatgt tcccgaatca
    42421 cccgaacctg ctgcccgctt attttgcgga agatgatcat ccgcaaatgg aaaaatatgt
    42481 ggttaaaccg atcttctccc gtgaaggcgc aaacgtgtcg atcattgaaa acggcaaaac
    42541 cattgaagca gcggaaggtc cgtatggcga agaagggatg attgttcagc aattccaccc
    42601 gttaccgaaa ttcggcgaca gctatatgct gattggtagc tggctggtga acgatcaacc
    42661 cgccggaatt ggcattcgtg aagaccgtgc attgatcacc caggatatgt ctcggtttta
    42721 tccacatatt tttgttgagt aatcaatgat gccggatggc attcgccatc cggtaattgt
    42781 taacctatct gcaccgacag catactcagg ctgcccattt ctataccatc gaccggaatg
    42841 gtaattggct cctgcccatc ccacgcacct aagacataca acaacggcag atagtgctcc
    42901 ggcgttgggt tcgataacgc gccaccttca tggtcgaggt aattcaccag aggatgctgc
    42961 tctaccgccc cttgccacgt cagattcgct ttcacatact cattaaacga catcgcccaa
    43021 ggatatggtg aactatcacc gtgccacttc actgtacgca ggttatgcac cacgttgcca
    43081 ctggcgacca acattattcc ttcatctcgc agcgctgcca gtttgcgccc catttcgaaa
    43141 tgccaggcgg caggtttgct actgtcgata ctcaactgca ccatcgggat atcggcgtca
    43201 ggatacatct taatcagcac gccccaggaa ccgtggtcaa agccccaggc ttctttatcc
    43261 agcgtcaccg ggaccggtgc taacagctca accagatgct gtgccagcac aggcgaaccc
    43321 ggagcaggat aatgcgtatc gtacagcgcc tgcgggaagc caccaaagtc atgaatcgtg
    43381 ggcggcgtct ccatcgcggt cactcctgtt ccacgggtaa accagtgagc cgaaaccacc
    43441 acaatcgctt gcgggcgtgg caatgtcatc cccaacttct gccagctgcg ggtatacaaa
    43501 ttatcttcca gcacgttcat cggactaccg tgacctaaaa acaatgctgg catacgtgtt
    43561 gaagacatga tgatatcctt aactaaaggt gtcattttga tatcctcaca atacgcttgt
    43621 tcggcggagt aagaacccgg ataacaatga tgatgatcat cagttatttt gacgatctgc
    43681 ctgaaggtga agatttataa ggagttgtcg atgtcagtac ctctcattct gaccatactg
    43741 gcgggggcag ccacgtttat tggcgcgttt ctcggcgttc tcgggcaaaa accctcgaac
    43801 cgcttactgg cgttttcgct aggatttgcg gcggggatca tgttgctcat ctcattaatg
    43861 gaaatgcttc ctgccgcact agcagctgaa ggaatgtcgc ctgtgttggg ttatggaatg
    43921 tttatcttcg gtctgcttgg ctattttggc ctggaccgca tgttgccaca tgcgcatccg
    43981 caggatttaa tgcaaaaatc ggtgcagccg ttgccaaaat cgatcaagcg cacagccatt
    44041 ctgctcactc tcggcatcag tctgcataac ttcccggaag ggattgccac ctttgtcacg
    44101 gcgagcagca acctggagct gggatttggc atcgcactgg ccgtcgcgtt gcacaatatt
    44161 cctgaaggtc tggcggttgc aggcccggtt tatgcagcaa cgggttctaa acgcacggca
    44221 attctgtggg cggggatttc tggattggca gaaattcttg gtggtgtgct ggcgtggtta
    44281 atcctcggta gcatgatttc ccccgtggtc atggcggcaa tcatggcggc ggttgcagga
    44341 attatggtgg cgctctcggt tgatgaatta atgccgctcg ccaaagagat tgaccctaat
    44401 aataacccca gctatggcgt actatgtgga atgtcagtga tgggattcag tttagtgctg
    44461 ctacaaacgg cgggaattgg ttaaaagcaa catcagaagt tggccagcgc atggacaact
    44521 tctgctagct tcactaaagc cttattctac cttttcggct gtctcttctc tcgtactgtt
    44581 taagtatttg ttccgcgctt caagcagtgc gtctctgtcc tggcgaaacg cccgacaata
    44641 acgtttcaaa tgcaggaaat aacccaccac aacaagagca atcaatacaa tccagtacca
    44701 ggcgataaac atccttttac ccttaatatt taacaatact aaacatttag cgcataaatt
    44761 tcacatatcc tttttcggat atattcatat ggtcgcataa taacggaaca attatatgat
    44821 tttgatcata atgaaaaaga atgcctgata ttaatgaaat tttctcatag aaagagaagc
    44881 ggaattaaca tcttgcatag cagggcaaaa taaaaaccga agccagctac aacttcggtt
    44941 tcttgattaa ggcagtaatt aagcagcggt tttcagctgg ctttacgctc atgtgcctga
    45001 cggtatgcca ccaggtcttc aatagtcacg agcgccatat tgtgtttatt ggcaaactca
    45061 atgcactctg gtgcacgcgc catcgtgcca tcgtcattag tcaactcaca cagcacacca
    45121 gccggtttaa agcctgccag cgtcatcaaa tcaatagtcg cttcagtatg accgccacgc
    45181 gtcagtacac cacctgcctg cgcgcgaagt gggaaaacgt ggcccgggcg attcagatca
    45241 gaaggttttg cgccatcggc aatcgctgcg cgaacggttg taatacgatc agcggcagaa
    45301 acaccggtag tcacaccttc agccgcttca atggtcacgg taaaaccggt gccataggcg
    45361 ctggtgttat tttctaccat cattggcaga tcaagttgtt tacggcgatc ttcagtgatg
    45421 cacaggcaaa caataccgct accgtggcga atggtcagcg ccatctgctc aacggtcatg
    45481 gtttctgccg ggaagatcat atcgccttcg ttttcacggt cttcatcatc aagcaccatt
    45541 acaccgcgtc cttcacgcag cgcagccagt gcattttcaa cacgttcgaa aggcgtacca
    45601 aaagaggaaa gtagcgtctg attcatggta aaaaaacctc actaaaatta tggttaccag
    45661 aatcagggca gtcttaggag tggcggcata tagccaaaat aacgtgagcg ggtccatgcc
    45721 cgacagaatc gttactctct cccatccgga ctctaaccgt cggccccgga attacaccgg
    45781 atctgctgtc ctttgagttc gcacccaaag cgctcgcggg ctttcaactg agttgattta
    45841 ccgccggtgg ggaatttcgc cccgccctga gaataagcgg attcactata acgctaatga
    45901 ttagcggcag caacgcatag cttcacataa ttctggttta tgacttaccc ttatcgcact
    45961 acaatggcac tcaacaccta tcagtacagg gaaactacaa tgattgaccc gaaaaaaatt
    46021 gagcaaatcg ctcgccaggt tcacgaatca atgcctaaag gaatcaggga gttcggggaa
    46081 gatgtggaga aaaaaatccg ccaaacccta caagctcagc tgacgcgcct cgaccttgta
    46141 agccgtgaag agttcgacgt ccaaacgcaa gtcctgttac ggactcgtga gaaactggcg
    46201 ctgcttgaac aaagaatgag cgaactggaa aatcgcagta ctgagattaa aaagcagcca
    46261 gatccagaga ctctcccccc aacgctgtaa acatttgtct ttaatgcact gtaaattatg
    46321 aattaatcct ctaatttaca gtgctttcta ttaccttatc gcttaattcc attcagaata
    46381 tcatcacaaa aatgaatgag tgcaattgaa aatataattg ttatgtattt tttaagtttt
    46441 acttaatcca tatttagata aactaatttc ataaaacccg aaaagtaaaa tataatattt
    46501 gaatgattag caatccttaa ataacagtta ctaatcattc attttctact tatatttata
    46561 taaaggaata ttatgtctgc ttttaaaaag tcacttcttg ttgctggcgt agcaatgatt
    46621 ctttccaata acgtatttgc tgacgaaggt cacggcattg ttaaatttaa aggtgaagtt
    46681 atctctgctc catgttcaat taaaccaggt gacgaagact taactgtaaa tctgggtgaa
    46741 gtagcagata ccgttctgaa aagcgaccag aaatctttgg ctgagccttt cactattcac
    46801 ctgcaagact gcatgttgag ccagggtgga accacttatt caaaagcgaa ggttactttc
    46861 accacagcga atactatgac gggtcagact gacctgctga aaaacaccaa agaaactgaa
    46921 atcggtggtg ccacaggcgt tggcgtacgt attctggata gccagagcgg cgaagtgact
    46981 ttaggtactc cggtagtaat tacattcaac aatactaaca gctatcagga actgaacttc
    47041 aaagctcgta tggaatcccc aagcaaagat gctaccccgg gcaacgttta cgctcaggct
    47101 gactataaga ttgcttacga gtaatcacat taggccgggt tcgcccggcc acatcattaa
    47161 cggattaatg ataagtggaa cagatgtata aaaaattaaa attaaccaca ataagcgaat
    47221 tgattaaaaa tatttattgt tcattatccg ttctcatcat tggttgtgcg tcagcttatg
    47281 ccgttgaatt caacaaagat ttaatcgaag ccgaagatcg tgaaaacgtt aacctttccc
    47341 aatttgaaac tgatggccaa ttacccgtag gtaaatactc attaaacgct cttattaata
    47401 ataaaaggac gccaatccat ctggacctgc aatgggtatt aattgataac caaactgcag
    47461 tttgtctgac tccagagcaa ttaacattat taggctttac tgatgagatt attgaagtgg
    47521 ctcagcaaaa cctgatcgat ggttgttacc ctatcgaaaa agaaaaacaa attacaactt
    47581 atctcgataa agggaaaatg caattatcca tatctgcacc tcaggcatgg ttaaaataca
    47641 aagatgcaaa ctggacgcct cctgaacttt gggatcatgg tattgctggg gcatttcttg
    47701 actacaattt atatgcctct cattatgcac cacatcaggg cgataattca caaaatataa
    47761 gttcctatgg gcaggctggg gttaatcttg gggcctggcg cctgcgtact gattaccagt
    47821 acgatcaatc atttaacaat ggcaaaagcc aggcgaacaa cctggatttt ccgcgtattt
    47881 atttgtttcg cccaatccca gcaattaatg caaaactaac tataggtcaa tacgatactg
    47941 aatcctctat tttcgactct ttccattttt ctggcgtttc gttgaaaagc gatgagaata
    48001 tgttgccgcc tgatctacgt ggttacgcac cgcaaatcac gggtgttgca caaacgaatg
    48061 caaaggtcac tgtctcacag aacaaccgta ttatttatca agaaaatgtt cctcctggcc
    48121 catttgctat tactaattta ttcaatacat tacaggggca actggacgtc aaggttgaag
    48181 aagaggacgg gcaagtcacg caatggcaag ttgcatctaa tagtattcct tatctgacgc
    48241 gtaaagggca gattcgctac accactgcta tgggtaaacc gaccagcgtt ggtggtgatt
    48301 ccttacaaca acccttcttc tggactggtg aattctcatg gggttggctg aacaatgtat
    48361 ccctgtatgg tggttcagtt ttaacaaacc gtgattatca atctctggct gccggcgttg
    48421 gctttaacct taactcattg ggttcattat cttttgatgt cacacgatct gatgctcagt
    48481 tgcataatca ggataaagaa acgggttata gctaccgcgc taactattca aaacgttttg
    48541 aatctaccgg tagccagctc actttcgctg gttaccgttt ctctgataaa aactttgtga
    48601 caatgaatga atatatcaat gacactaacc attacacgaa ttatcagaat gaaaaagaga
    48661 gttatattgt cacgtttaac cagtatcttg aatcattaag gttaaataca tacgtaagtt
    48721 tggctcgtaa tacttactgg gacgccagca gtaatgtgaa ttattcattt tcacttagcc
    48781 gcgattttga tatcgggcca ttaaaaaacg tctccacttc tctaacattt agccgaataa
    48841 actgggaaga agacaaccag gatcaactgt acctaaatat ttcgattccc tggggaacca
    48901 gtagaacatt gagctatggt atgcaacgaa atcaggataa caagatttcg catactgctt
    48961 cgtggtatga ctcttccgat cgaaataatt cctggagcgt ttctgcttca ggcgacaatg
    49021 atgaattcaa agatatgaaa gcgtcactac gcgccagtta tcagcataat accgagaacg
    49081 gtcgtctcta tctctccggg acatcacagc gagacagtta ttattctctg aatgccagtt
    49141 ggaatggttc attcacggcg actcgtcacg gagccgcttt ccatgactat agtggtagtg
    49201 ctgactcgcg ttttatgatc gacgcagatg gtgcagaaga tattccgttg aacaataaac
    49261 gcgcggtaac taatcggtat ggcatcggag ttattccatc agtcagcagt tacataacaa
    49321 catcattaag cgttgatacc cgaaatcttc cagaaaatgt ggatatcgaa aactcggtta
    49381 tcaccaccac cttaaccgaa ggtgctattg gctacgccaa acttgatacc cgcaagggct
    49441 atcaaatcat gggggttatt cgcctggcag atggtagtca cccaccactg gggattagcg
    49501 taaaagataa aaccagccat aaagaattag gactggttgc tgatggcggc tttgtatacc
    49561 tcaacggcat tcaggatgac agcaaactta ctttacgctg gggtgataaa tcctgtttta
    49621 ttcaaccacc caatagcagc aacttaacca ccggaacggt tattttaccg tgtattagcc
    49681 aaaattaatt caggatgaaa ctaatgcgct acttaaacac aaagaatctc attgcagcag
    49741 gagtattact cgcctgcatg agcagcattg cctggggtgc catcatcccg gaccgtaccc
    49801 gtatcataat gaatgagtca gataaagggg aagcattaaa acttactaat caaagcaaaa
    49861 atctacccta tctggcgcaa acatggatag aagatacgaa aggcaataaa tcacgtgact
    49921 ttattgtcac ggttccccct atggtacgtt taaatcccag tgagcaaatt caaataagaa
    49981 tgataagtca ggaaaaaatt gctcaactgc ctaatgacag agaaacgctc ttctatttta
    50041 acgtgcgaga aataccgcca aaaacagata aaaaaaatgt gatgcaagtc actatgcagc
    50101 acgcattgaa actattctgg cgaccaaaag ctattgaatt agaagatgat ggggtaatgt
    50161 cttacgaaaa agttgaaatc atccgtagaa atgatggttc gatccgcttt aacaataaga
    50221 tgccttatca cgttacgctg ggttatatcg gaacaaacgg aatcacaatg ttgccacaaa
    50281 cgcaaagttt aatggtaacc ccttatagcc atgcagatac gcagtttaaa aatgttccgt
    50341 cagcttttca ggttggttat atcaatgatt ttggtggatt aagtttttat gaaataaact
    50401 gcccgacggt taataactct tgtaatgttt ccgtagccaa acgagataaa taatatgcgc
    50461 tacttattaa ttgtaattat atttattata ggttttagtg cattacctgt atgggcaatg
    50521 gactgctatg ctgaacatga agggggaaat accgtagtca ttggttacgt accaagaatt
    50581 tctatcccca gtaatggtaa aaaaggtgat aagatctggc aaagcagtga gtattttatg
    50641 aatgtcttct gtaataatgc attacctgcc ccatcacctg gagaagaata cccatcagcc
    50701 tggactaata taatgatgtt tttaccaggc ggtcaggact tttataatca gaactcctat
    50761 atttttggcg taacatacaa tggagttgat tatgattcga ctgcccctaa tgctatcgca
    50821 gcaccagaat gcattgatat aaaaggagcg ggtaccttta ataatcacta taaaaacccc
    50881 gctgtctgta gtggaggtcc tgagcctcaa ttatcagtaa cctttccagc tcgcgtacaa
    50941 ctctatatta aattagcaaa aaatgccaat agagttaata aaaacctggt gttacctgat
    51001 gaatatatcg ctctagagtt taaaggtatg agtggcgcag gagctataga agtagacaaa
    51061 aatttgacct tcaggattcg tggattaaat aatattcatg tccttgactg ctttgttaat
    51121 gtcgcactgg aaccagctga tggcgttgtt gactttggta aaataaattc ccgaacaatt
    51181 aaaaatacca gcgttagtga gacgtttagt gtagtcatga caaaagatcc tggtgcggcc
    51241 tgtactgagc aattcaatat tttagggagc tttttcacta cggatatttt gagtgattat
    51301 agccatctgg atatgggtaa tggactgcta ttgaagatat ttcataaaga tggaacagca
    51361 acggaattta accgcttctc acaatttgct tctttttcat cgtctagtgc accttcggtc
    51421 accgcaccat tcagggcaga acttagtgcg aatccggcag agactgttgt tgagggaccg
    51481 tttagtaaag acgtcatcct caaaatcacc tataactagc atctaataca aacactaaaa
    51541 cgggccatca ggcccgttat atcagtgctc taactccagc tctcttgcct gcatcatctg
    51601 ttgacaatac caggcataac gtgcgcaaaa ccaggttcta tgttcttcat ccatgttacc
    51661 agtaacggca agaatatcga ccgggcgacc ttctgcgtgc attctggcaa aactacgcgc
    51721 caggaaatcg aaattattta aagaattaag actatgatcc ataagcacat cctccggtta
    51781 tcgcatacct tttaaggcca tatgcttttt ctgatttcag tattccccaa tcagtgtagt
    51841 gcctttaaac ataacgtgcg taaatatatt gtccccgatc acacttttta gtgaaaaata
    51901 aaaaaccata ttaaaaaact ttctcttcca gaacttctct aacatgtgtt attattaaca
    51961 aagataaatg taagcaagga aactatccca aagtaaatat tgcgtcacta aatggacatt
    52021 ggagtgacat atgattttat tcgccgacta taatacccct tatcttttcg ctatttcctt
    52081 tgtactgttg atcggccttt tagaaatttt agcgcttatc tgcggtcaca tgctctccgg
    52141 tgctcttgat gcacatcttg accattatga ttccataacc acaggtcata ttagccaggc
    52201 acttcattat ctcaatatcg gaagattgcc agctctcgtc gttctctgct tgctggctgg
    52261 tttttttggt cttattggca tcctgttgca gcatgcctgc atcatggtct ggcaatcacc
    52321 actgtcgaac ctgttcgtgg ttcctgtcag tttactgttt acgattattg cggtgcatta
    52381 taccggtaaa atagttgcgc cctggattcc acgcgaccat agttcagcta ttacagaaga
    52441 agaatatatt ggtagcatgg cattaattac cggtcatcaa gccacttcag gtaatccctg
    52501 cgaaggaaaa ctcaccgatc aatttggcca aatacattat ttactactgg agcctgaaga
    52561 aggaaaattt ttcactaaag gagacaaagt gttaattatt tgccgacttt ctgcaacgcg
    52621 atatttggcg gaaaacaatc cctggccaca aatattgtaa tttattaatc aaaaggaaat
    52681 ttaataatgg atgatattgt taattctgtg ccctcctgga tgtttaccgc gattattgcc
    52741 gtatgcattc tgtttattat tggaattatt ttcgccaggc tctatcgtcg cgcttcggca
    52801 gagcaagctt ttgttcgtac tggtttaggt gggcaaaaag tggtgatgag cggtggcgca
    52861 atcgtgatgc cgatctttca tgaaataatc cccatcaata tgaatactct gaagctggaa
    52921 gtcagccgct caaccattga tagcctgatt acgaaagatc gtatgcgtgt cgatgtagta
    52981 gtcgctttct ttgtacgggt aaaaccgtct gtagaaggca tcgccactgc ggcgcagacg
    53041 ttggggcaac gcacgctatc accagaagac ttacgtatgt tggttgaaga taaatttgtc
    53101 gatgccctcc gtgcaacagc tgcacaaatg accatgcatg agttacagga tacccgcgag
    53161 aacttcgtgc agggagtaca aaatactgtc gcagaagacc tgtcgaaaaa cggtcttgag
    53221 ctggaaagcg tttcacttac caactttaac cagacctcga aagaacattt caatccgaac
    53281 aatgcctttg acgccgaagg tttaaccaaa ctgactcagg agacggagcg ccgtcgtcgc
    53341 gaacgtaacg aagttgaaca ggatgtagaa gttgcggtgc gtgagaaaaa ccgtgatgca
    53401 ctttcgcgca agttggagat tgaacaacaa gaagcgttta tgacgcttga gcaggagcag
    53461 caggttaaaa cccgtactgc cgaacagaat gcacgtattg cggcttttga agctgaacgt
    53521 cgtcgtgaag cagagcagac gcgaattctg gctgaacgac aaattcagga aacggagatc
    53581 gagcgcgagc aggccgtccg ctcaagaaag gttgaggctg aacgtgaagt tcgtattaaa
    53641 gagatcgaac aacagcaggt caccgaaatc gccaaccaga cgaaatcgat cgctattgcc
    53701 gccaaatcgg aacaacagtc ccaggcagaa gcgcgtgcta accttgcact cgcagaagca
    53761 gtaagtgcgc aacaaaacgt agaaaccact cgccagaccg cagaagccga tcgtgctaaa
    53821 caagttgccc taatcgctgc cgcgcaggat gcagaaacca aagcggttga actgaccgtg
    53881 cgggcgaaag cagaaaaaga agccgcagag atgcaggcgg cggctatcgt tgagttagcc
    53941 gaagctacac gtaaaaaggg tctggcggaa gcagaagcac aacgtgcgct gaacgatgct
    54001 atcaacgtac tttctgatga acaaaccagc cttaaattca aactggcctt gttgcaggcg
    54061 ctacctgcgg taatagaaaa atccgttgag ccgatgaaat ctattgacgg tatcaagatt
    54121 attcaggtcg atggtctgaa tcgtggcagc gctgcgggtg atgcaaacac gggtaatgtg
    54181 gggggcggaa acctggcgga acaagcatta tcagccgctc tctcttaccg cacacaggca
    54241 ccgctgattg actccttgct caatgaaatt ggcgtttcag gcggctcact ggcggcattg
    54301 acttcaccct taacctcaac aactcccgtc gccgaaaacg tagaataaat tttcagctat
    54361 gaacgtccct ggcacaaaaa gccggggatt ttttatatct gcgttccgct aaaaactgca
    54421 aatgatcagg tcgttgcaac gttttgcgtg accgctcggg gaaggcaaaa ctgcctctgg
    54481 gaaagcgttg cgcgaggtcc ggcgctcatc aacaatcggg gagtcagcaa agggctgaaa
    54541 cgggaaagcc cctcccgagg aaggggcctt agggttatga tgaagctcgt catcatactg
    54601 attgtgttgt tactcgtaag tttcgcagct tattaacagc caatcagagg ggggagaaat
    54661 cctccctcac ccttgttcct ttactttagg ttgaaaaaaa tcagcgtcaa gaaggtttca
    54721 ttaacacata tccctggccc aaaaagccgg ggatttttta tatctgcgtt ccgctaaaag
    54781 gtgcaaatgc tcaggacgtt gcagcgtttt gcgtgaccgc tcggggaagg caaaactgct
    54841 tcagggaaag cgttgcgcgg ggtccggcgc tcatcaacaa tcggggagtc agcaaggggc
    54901 tgaaacggga aagcccctcc cgaggaaggg gccttataga aggaaagggt tatgatgaag
    54961 ttcgtcatca tactggttgt gttgttactg ttaagtttcc cgacttacta acaactgatc
    55021 agagggggga gaaatcctcc cttacccttg ttcctttact ttaggttgaa aaaacaacag
    55081 cgtcaatagg cctgcctgct acgaagcgag atctgtgaac cgctttccgg ttagcctttt
    55141 ttatcctgtt ggatcttctt gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc
    55201 accagcactt cgccgccgtt ggcccagact tctttactcc cggcaatttc ttctggttta
    55261 tagtcgccgc ctttcaccag cagatctggc aggatcccgg cgatcagacg ctgcggcgtg
    55321 tcttcttcaa acgacactac ccagtcgacc gcttccagtg cgcccagcac aatcatacgc
    55381 tgctcgagtg ggtttaccgg gcgggaatcc cctttcagcc gtttggtgga ggcatcgctg
    55441 ttcacagcga caatcagacg gtcacccagt ttgcgagcat ttgccagata ggagacgtgc
    55501 ccggcgtgca ggatgtcaaa gacaccgttg gtcattacca ccttttcgcc gcgtttacgc
    55561 gctgccgcta cagcaagctt cagttcctct tcggtcatca caccaaagcc ggtatctgcg
    55621 cgcccacgca ctgcattttc cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg
    55681 accaccacgc cagccgccgc attggcaaag aagcaggctt cttccagcga attacccgct
    55741 gccagcgttg ccgccaggac gccaatcacc gtgtcgcccg caccggtaac gtcatacact
    55801 tcctgcgcct gggttggcat atgcagcggc gctttacccg gttgcagcag cgacataccc
    55861 tgttcggaac gggtcactaa cagagccgag agttcgtaat cggcaatcag tttcatgccg
    55921 cgctcaacaa tctcttcttc ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga
    55981 ttcggcgtta acagcgtagc gccgcggtag cgctcaaaat cggtaccttt tggatcaatc
    56041 agcaccggaa cgcccgcttt acgcgccagt tggatcatct gctgtacgct tgccagcgca
    56101 cctttggcgt agtcagaaag caccagcgcg ccaatcgaac tcagcgcctg attaatccgc
    56161 tcgtgcagcg gctgcggatc aacaccttcg aaaccttctt caaaatccag gcggatcagc
    56221 tgttggttgc gggaaagtac ccgtaatttg gtgatagtcg gatgcgtcgg tacagaaacg
    56281 aagtcgcatt tgacgttgac gtcggccaga gatttactca gcgcacgcgc cgcatcgtca
    56341 atgcccgtca gcccgaccag gcgagcattg gcaccgagag aagcgatgtt cattgccacg
    56401 ttagccgcgc cgcccggacg ttcttcgatg gtattgactt taaccacggg caccggcgct
    56461 tccggcgaga tacggctggt gggaccgtac cagtaacgat ccagcatcac atcaccaacc
    56521 accatcactc ctgcacgttc aaactctggc agcgttactt tcattcctgt ctcctgagag
    56581 attcaaaatt tgcgcgcgat aataccatac ttcattcttc caccagccac ttctgccagc
    56641 ttgcccgcac cagttcacgc tctgcggtga agcaatcctc cggcacatgg cccggcaatt
    56701 cctgtaatgc cagatgatga agttcatcgc gcagcgtagt ataagcacgg gtcagcgcca
    56761 tcgcttcctg ctcttccata atgtcgtttt gcgccagtag ttccagaatg cgcacgttgt
    56821 ctgaccagcg cgttaacttc ggtttttcat gagcgtagcg caacaccaga tattgggtaa
    56881 taaattcgat atcggtaatt ccgccttcat cagctttgat atcaaagcga tcgcgatgtt
    56941 tattgccgag atgagcgcgc attttctcgc gcatttcccg cacttccgtt tgcagagttt
    57001 taccttcacg cggcagcgtc ataatctcgc gacgcaccgc gtcaaagtgc gcggtgagct
    57061 gcggatcgcc gtacactacc cgcgcacgca ccagcgcctg atgttcccac gtccaggcct
    57121 cgtttttctg ataatcggca aatgcttctg cggatgtcac cagcattccc gcggccccgg
    57181 acgggcgtag ccgtgcatcc acttcataca aaatgccgga agaggtacgc gtactgaaca
    57241 gatgcataat gcgttgcgcc agacgcagat aaaactgtcg cccgtcgatt tcccgctcgc
    57301 cgtcagtcat cgcatccatt gggcaatcat ggaggaagat caggtcaaga tcggaactgt
    57361 agcctaactc ccagccgccc agcttgccgt agccgaccac cgcaaaacca cggccttcgc
    57421 gatcgttgag gtgatttggc ttaccgtagc gggccaccat ttgaacccac gcctgctgaa
    57481 cgacggcatc tatcatagcc tctgccagcc aggttaagtg atcgctcact ttcatcaccg
    57541 gtagcgtacc agcgatatcc gctgcggcga tgcgtaacag ctgcgcctgt ttgaactgac
    57601 gcagcgcctc aagctgttgc tcttcgtcat cttccggcac gcgcagcaaa tactggcgca
    57661 actcatcgcg gtaggcatcg gtcgccgtcg gctggtaaag ggtgtttgga tcgagcaatt
    57721 catccagcaa taatggataa cgcgccagtt ggctggcaat catcggcgac gcggcacaca
    57781 gagaaatcaa atgtttaagc gccgcgggga attcactgag caattctaaa taggtggtgc
    57841 gggtaacaat ccccaccagc aaggcggtaa tgcgcgacag cgtaacggca gcgtcttcac
    57901 gcgcacagac atcacttagc agatgcggca tcagatggtc gagcacctga cgtcctcgcg
    57961 gcccgatggt gcgcttatcc agctctttgc ggaaatcggc aatcagcgtt agcacctgtt
    58021 tgcgatcatc ctcgctaaga tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat
    58081 cctgccacag ctcacgccac tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt
    58141 cgccaatcaa ttcattaaac acccggcgca cattggtcat atgtgcggtc agcgccccgg
    58201 tcagttgcgg ccagtcagca aagtccatcg cccacgccag ccgcgcacgg ttaagctcat
    58261 cagaaggaag cgtctgggtt tgttcgtcgt taatgctttg cagcaggttt tccagacgcc
    58321 gcaggaacag atacgccact cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg
    58381 cggcaatgac gctgagcgtt ggcagtaaag agcgcgactg cagcgacggt tcgcgcccac
    58441 cgcgaatgag ctggaacacc tgaacgataa attcaatttc gcgaatgccg cctgcgccga
    58501 gtttgatatt gtcggtcaaa ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt
    58561 tgcgcagcga ctgaatcacg ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca
    58621 tcgcacgcaa ctcgttagca tagacgcctt cgctatcgcc cataatccgc gccttgacca
    58681 tcgcgtaacg ctcccagtcg cgcccctgct cctggtaata atcctccaac gcggcaaagc
    58741 tcagcaccag cgggccactt tcgccaaacg gacgcagccg catatccacg cgatagacga
    58801 agccatcctg cgttggttga tccaacactt taatcagccg ctgccccatg cgggtaaaaa
    58861 actgcgcgtt atccagttcc cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa
    58921 aaatcagatc gatatctgag gagaaattca gttccccacc gcccagctta cccatgccta
    58981 aaatcagcag cggttgcgct tcgccctgcg cattgcacgg cgttccccac tcgcggcagc
    59041 aagcgtcata cagccagtca cgcgcggcga caatcagcgt ctccgccaga tggctgagct
    59101 gctgcaatat gctctcttca gtaaccaaag ccagcgtttg cgcccaggcg atgcgcacca
    59161 taatgcgccg ccggaatagc cgcagctcgc gcattaaccc ggcttcgtca ctcacattac
    59221 tgagcgcctc ctgcaaccat gccgcgtaat gctgccattc gtcggcctgc ggcggttggc
    59281 tttccagttc cgtcagccac tctggatgca caatcacgct gtcctgcaca aaatcactaa
    59341 aagtaagtac tgacttcgcc tgtgcgctaa gtgattcctc ggctaaaggc tctggcagcc
    59401 gctcaacaac ggtctgccag tactgctgta acggtgaaga gagcggcttc ataagcgatt
    59461 ttatccttgc ctgattaacg ttttccgctg tgcaaccaga acggttcctg attgtttgcc
    59521 tcattacgga aatgttcaat ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc
    59581 tgccagttct ccagccaggc ttgcgcgacg acaggatcat agtaacctgc cagcagcagt
    59641 attgagtcaa tatcacgcgt caggcgtggc aactgatcat ggtagcgatc gcctaacggc
    59701 tggcagaaaa cgcttttcag ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt
    59761 ttgaaggaat cgctgatttt accctgcgct ttggcatcta aaaacggctg ccaggctttg
    59821 cttaccaacc attcggtcaa cgccagcttt gccattgccg tttcggtaga gtagaccgcc
    59881 gtcacggcag aaaccgcaga agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag
    59941 tgagtgctcg ctttacgcgg cacaatgcca ccgaacagca tcagtgcatg gcgaacaagg
    60001 ccaatggctg ccagcacctg ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca
    60061 tgatactgcc attgcgctaa cgccagctcg aacgccgctt ccagcccctg ttccacatcg
    60121 gcttttgccg caacatgcaa aatggtggtc ggtttgattt cacgcgccgg attgccctgc
    60181 gccagatgat agccacgcgc cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat
    60241 accagttggt tcgccagttt cagcaccgcg cgcgtgtcgc cgctaagcag ttccagttcc
    60301 agctcacaga taggttcagc aaattccccc gctttcacct ccccctgatc gagagcgatt
    60361 tcaattcggc tgtcatcgac cgccaccagc catttttcgc gataaaaatc ggtgctgaac
    60421 agcggttgta cgcgggaagc gagatcggcg ggcaattcgc cgttcggcca gacttccgtc
    60481 ggtaactgcg ccaggtcgag cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg
    60541 tgtaagccgc ctgtcactct tcctgcaact ttcatggtca tctcatagcg accgttttcg
    60601 ccacgaatac gtaagcccat atcgtgccca cgcagccagt tatccggcgt ttcgtagtaa
    60661 atattcagca actgcacggg gtcatggtgc tcgccgccca gcgtattgag atggtcacgc
    60721 aacgcctcaa cggcactgtg attaacaata aactttaatt cgatttcctg agccatggcc
    60781 tttacttatc ggttatgtca cataagtgac gatgaacggc gaacttaatg cgatcttttt
    60841 tgtcagtaga tagtattttg cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa
    60901 agtttgaagc agtggcaaga cctgacacag aggattccga ttgatgtcga atgctttgcg
    60961 tagcgacact gatgccacta ctatcgttcc actttccatg acaataacga cagcctgatg
    61021 ccaaaattac gcctgatcgg attaacttta ctcgcactta gcgccactgc cgtctcacac
    61081 gctgaagaaa cgcgctatgt ttctgatgaa ctgaatacct gggtccgtag cggtccggga
    61141 gatcattatc gcctcgtggg cacggttaac gccggcgagg aagtgacctt attacaaact
    61201 gacgccaaca ccaattatgc ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg
    61261 ttgaaacaac ttagcactga gccaagcctg cgctcccgtg tgccagatct ggaaaatcag
    61321 gtcaaaaccc tgaccgataa actcaccaat atcgataaca cctggaatca gcgcaccgca
    61381 gaaatgcagc aaaaagtggc gcagagcgac agcgtgatca acggattaaa agaagaaaat
    61441 cagaaattga aaaacgagct gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag
    61501 ttggatgaca aacagcgcac catcatcatg caatggttta tgtatggcgg tggcgtgctg
    61561 gggcttggct tgctgctcgg tctggtactg ccacacctga tcccaacgcg caaacgcaaa
    61621 gatcgctgga tgaactaaat cgccttcttc gttgcactgt cttattatta ggaattgtgt
    61681 atttgacgaa gggatgatgg cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca
    61741 ttgttagggc taccggtcaa agacagagat tgggtggtgg tcggcagtac gccacaggag
    61801 atgctcgacg cgggctacca gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa
    61861 acgcatgaag agtatgcgct ggcacgtacc gaacggaaat ccggttccgg ttacaccggt
    61921 tttacttgct atgccgcacc ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg
    61981 accattaatg cgctggccca ggacgataac ggcgagatta tcgacccgta caacggtctg
    62041 ggcgatctgc aaaatcgtct gttgcgccat gtttcccccg cttttggcga agatccgtta
    62101 cgcgttctgc gcgtggcgcg ttttgctgcg cgttatgccc acctcggttt tcgtattgcc
    62161 gatgaaactc tgacgctgat gcgcgagatg acccatgcgg gtgaactgga acacctgacg
    62221 cctgaacggg tatggaaaga gacggaaagc gccctcacca cccgcaatcc acaggtgttc
    62281 ttccaggtac tgcgcgattg tggcgcactg cgcgttttat tcccggaaat tgacgcactg
    62341 tttggcgttc cggcccctgc caggtggcat ccggaaatcg acacgggtat tcatacctta
    62401 atgacgctct caatggcggc gatgctgagt ccgcaggtcg atgtccgttt cgcaacttta
    62461 tgccacgatc tcggtaaagg actgacgccg ccagaacttt ggccgcgtca tcatggtcac
    62521 ggtccggcgg gtgtgaaatt agtggaacaa ctctgccagc gtctgcgcgt gccaaatgaa
    62581 attcgcgatt tagccagact ggtggctgag tttcacgatc tcatccacac cttcccaatg
    62641 ctgaacccaa aaactatcgt caaattgttt gattccatcg atgcctggcg taaaccgcag
    62701 cgtgtcgagc aactggcgct gaccagcgag gctgacgtgc gcggcagaac cggttttgaa
    62761 tcagcggact acccgcaagg ccgttggttg cgcgaagcct gggaagtggc gcagtcagtg
    62821 ccgacaaaag cggtcgttga agcgggattt aaaggtgtgg agattcgcga ggagctgacc
    62881 cgacggcgga ttgcggcgat agccagttgg aaggaacaac gttgcccaaa gcctgactga
    62941 gtataaaact aaacgttaac acgaaccatc ccctcgcccc ttcggggaga gggttagggt
    63001 g
//
LOCUS       DAZGSP010000012        62894 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZGSP010000012 DAZGSP010000000
VERSION     DAZGSP010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.012"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(53..1297)
                     /gene="mtr"
                     /locus_tag="ACHK74_000894"
     CDS             complement(53..1297)
                     /gene="mtr"
                     /locus_tag="ACHK74_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFP0219757.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(1451..3340)
                     /gene="deaD"
                     /locus_tag="ACHK74_000895"
     CDS             complement(1451..3340)
                     /gene="deaD"
                     /locus_tag="ACHK74_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFP0219758.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(3333..3413)
                     /gene="yrbN"
                     /locus_tag="ACHK74_000896"
     CDS             complement(3333..3413)
                     /gene="yrbN"
                     /locus_tag="ACHK74_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFP0219759.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(3520..4404)
                     /gene="nlpI"
                     /locus_tag="ACHK74_000897"
     CDS             complement(3520..4404)
                     /gene="nlpI"
                     /locus_tag="ACHK74_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFP0219760.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(4513..6648)
                     /gene="pnp"
                     /locus_tag="ACHK74_000898"
     CDS             complement(4513..6648)
                     /gene="pnp"
                     /locus_tag="ACHK74_000898"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFP0219761.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(6895..7164)
                     /gene="rpsO"
                     /locus_tag="ACHK74_000899"
     CDS             complement(6895..7164)
                     /gene="rpsO"
                     /locus_tag="ACHK74_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFP0219762.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(7313..8257)
                     /gene="truB"
                     /locus_tag="ACHK74_000900"
     CDS             complement(7313..8257)
                     /gene="truB"
                     /locus_tag="ACHK74_000900"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFP0219763.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(8257..8658)
                     /gene="rbfA"
                     /locus_tag="ACHK74_000901"
     CDS             complement(8257..8658)
                     /gene="rbfA"
                     /locus_tag="ACHK74_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFP0219764.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(8822..11494)
                     /gene="infB"
                     /locus_tag="ACHK74_000902"
     CDS             complement(8822..11494)
                     /gene="infB"
                     /locus_tag="ACHK74_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFP0219765.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(11519..13006)
                     /gene="nusA"
                     /locus_tag="ACHK74_000903"
     CDS             complement(11519..13006)
                     /gene="nusA"
                     /locus_tag="ACHK74_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFP0219766.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(13034..13486)
                     /gene="rimP"
                     /locus_tag="ACHK74_000904"
     CDS             complement(13034..13486)
                     /gene="rimP"
                     /locus_tag="ACHK74_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFP0219767.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(13693..13769)
                     /locus_tag="ACHK74_000905"
     tRNA            complement(13693..13769)
                     /locus_tag="ACHK74_000905"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13733..13735),aa:Met,seq:cat)
     gene            14117..15460
                     /gene="argG"
                     /locus_tag="ACHK74_000906"
     CDS             14117..15460
                     /gene="argG"
                     /locus_tag="ACHK74_000906"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFP0219768.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(15468..17093)
                     /gene="yhbX"
                     /locus_tag="ACHK74_000907"
     CDS             complement(15468..17093)
                     /gene="yhbX"
                     /locus_tag="ACHK74_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP0219769.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(17552..17638)
                     /locus_tag="ACHK74_000908"
     tRNA            complement(17552..17638)
                     /locus_tag="ACHK74_000908"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17602..17604),aa:Leu,seq:gag)
     gene            complement(17653..17985)
                     /gene="secG"
                     /locus_tag="ACHK74_000909"
     CDS             complement(17653..17985)
                     /gene="secG"
                     /locus_tag="ACHK74_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFP0219770.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(18213..19550)
                     /gene="glmM"
                     /locus_tag="ACHK74_000910"
     CDS             complement(18213..19550)
                     /gene="glmM"
                     /locus_tag="ACHK74_000910"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFP0219771.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(19543..20391)
                     /gene="folP"
                     /locus_tag="ACHK74_000911"
     CDS             complement(19543..20391)
                     /gene="folP"
                     /locus_tag="ACHK74_000911"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFP0219772.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(20481..22415)
                     /gene="ftsH"
                     /locus_tag="ACHK74_000912"
     CDS             complement(20481..22415)
                     /gene="ftsH"
                     /locus_tag="ACHK74_000912"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFP0219773.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(22515..23144)
                     /gene="rlmE"
                     /locus_tag="ACHK74_000913"
     CDS             complement(22515..23144)
                     /gene="rlmE"
                     /locus_tag="ACHK74_000913"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFP0219774.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            23270..23563
                     /gene="yhbY"
                     /locus_tag="ACHK74_000914"
     CDS             23270..23563
                     /gene="yhbY"
                     /locus_tag="ACHK74_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFP0219775.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(23719..24195)
                     /gene="greA"
                     /locus_tag="ACHK74_000915"
     CDS             complement(23719..24195)
                     /gene="greA"
                     /locus_tag="ACHK74_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFP0219776.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            24443..25876
                     /gene="dacB"
                     /locus_tag="ACHK74_000916"
     CDS             24443..25876
                     /gene="dacB"
                     /locus_tag="ACHK74_000916"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP0219777.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSILASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(25916..27088)
                     /gene="cgtA"
                     /locus_tag="ACHK74_000917"
     CDS             complement(25916..27088)
                     /gene="cgtA"
                     /locus_tag="ACHK74_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFP0219778.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(27104..28069)
                     /gene="yhbE"
                     /locus_tag="ACHK74_000918"
     CDS             complement(27104..28069)
                     /gene="yhbE"
                     /locus_tag="ACHK74_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP0219779.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(28196..28453)
                     /gene="rpmA"
                     /locus_tag="ACHK74_000919"
     CDS             complement(28196..28453)
                     /gene="rpmA"
                     /locus_tag="ACHK74_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFP0219780.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(28474..28785)
                     /gene="rplU"
                     /locus_tag="ACHK74_000920"
     CDS             complement(28474..28785)
                     /gene="rplU"
                     /locus_tag="ACHK74_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFP0219781.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            29044..30015
                     /gene="ispB"
                     /locus_tag="ACHK74_000921"
     CDS             29044..30015
                     /gene="ispB"
                     /locus_tag="ACHK74_000921"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFP0219782.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            30243..30521
                     /gene="sfsB"
                     /locus_tag="ACHK74_000922"
     CDS             30243..30521
                     /gene="sfsB"
                     /locus_tag="ACHK74_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFP0219783.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(30569..31828)
                     /gene="murA"
                     /locus_tag="ACHK74_000923"
     CDS             complement(30569..31828)
                     /gene="murA"
                     /locus_tag="ACHK74_000923"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP0219784.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(31883..32137)
                     /gene="ibaG"
                     /locus_tag="ACHK74_000924"
     CDS             complement(31883..32137)
                     /gene="ibaG"
                     /locus_tag="ACHK74_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFP0219785.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(32297..32590)
                     /gene="mlaB"
                     /locus_tag="ACHK74_000925"
     CDS             complement(32297..32590)
                     /gene="mlaB"
                     /locus_tag="ACHK74_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFP0219786.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(32590..33225)
                     /gene="mlaC"
                     /locus_tag="ACHK74_000926"
     CDS             complement(32590..33225)
                     /gene="mlaC"
                     /locus_tag="ACHK74_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFP0219787.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(33244..33795)
                     /gene="mlaD"
                     /locus_tag="ACHK74_000927"
     CDS             complement(33244..33795)
                     /gene="mlaD"
                     /locus_tag="ACHK74_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFP0219788.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(33800..34582)
                     /gene="mlaE"
                     /locus_tag="ACHK74_000928"
     CDS             complement(33800..34582)
                     /gene="mlaE"
                     /locus_tag="ACHK74_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFP0219789.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(34590..35399)
                     /gene="mlaF"
                     /locus_tag="ACHK74_000929"
     CDS             complement(34590..35399)
                     /gene="mlaF"
                     /locus_tag="ACHK74_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFP0219790.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            35609..36586
                     /gene="yrbG"
                     /locus_tag="ACHK74_000930"
     CDS             35609..36586
                     /gene="yrbG"
                     /locus_tag="ACHK74_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFP0219791.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            36600..37586
                     /gene="kdsD"
                     /locus_tag="ACHK74_000931"
     CDS             36600..37586
                     /gene="kdsD"
                     /locus_tag="ACHK74_000931"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFP0219792.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            37607..38173
                     /gene="kdsC"
                     /locus_tag="ACHK74_000932"
     CDS             37607..38173
                     /gene="kdsC"
                     /locus_tag="ACHK74_000932"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFP0219793.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            38170..38745
                     /gene="lptC"
                     /locus_tag="ACHK74_000933"
     CDS             38170..38745
                     /gene="lptC"
                     /locus_tag="ACHK74_000933"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFP0219794.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            38714..39271
                     /gene="lptA"
                     /locus_tag="ACHK74_000934"
     CDS             38714..39271
                     /gene="lptA"
                     /locus_tag="ACHK74_000934"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFP0219795.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            39278..40003
                     /gene="lptB"
                     /locus_tag="ACHK74_000935"
     CDS             39278..40003
                     /gene="lptB"
                     /locus_tag="ACHK74_000935"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFP0219796.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            40051..41484
                     /gene="rpoN"
                     /locus_tag="ACHK74_000936"
     CDS             40051..41484
                     /gene="rpoN"
                     /locus_tag="ACHK74_000936"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFP0219797.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDDDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            41507..41794
                     /gene="hpf"
                     /locus_tag="ACHK74_000937"
     CDS             41507..41794
                     /gene="hpf"
                     /locus_tag="ACHK74_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFP0219798.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            41912..42403
                     /gene="ptsN"
                     /locus_tag="ACHK74_000938"
     CDS             41912..42403
                     /gene="ptsN"
                     /locus_tag="ACHK74_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFP0219799.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            42449..43303
                     /gene="rapZ"
                     /locus_tag="ACHK74_000939"
     CDS             42449..43303
                     /gene="rapZ"
                     /locus_tag="ACHK74_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFP0219800.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            43300..43572
                     /gene="npr"
                     /locus_tag="ACHK74_000940"
     CDS             43300..43572
                     /gene="npr"
                     /locus_tag="ACHK74_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFP0219801.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            43786..44418
                     /gene="yrbL"
                     /locus_tag="ACHK74_000941"
     CDS             43786..44418
                     /gene="yrbL"
                     /locus_tag="ACHK74_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFP0219802.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(44415..45143)
                     /gene="mtgA"
                     /locus_tag="ACHK74_000942"
     CDS             complement(44415..45143)
                     /gene="mtgA"
                     /locus_tag="ACHK74_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFP0219803.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(45140..45793)
                     /gene="elbB"
                     /locus_tag="ACHK74_000943"
     CDS             complement(45140..45793)
                     /gene="elbB"
                     /locus_tag="ACHK74_000943"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFP0219804.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(46023..48359)
                     /gene="arcB"
                     /locus_tag="ACHK74_000944"
     CDS             complement(46023..48359)
                     /gene="arcB"
                     /locus_tag="ACHK74_000944"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFP0219805.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKATLEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(48455..49384)
                     /gene="yhcC"
                     /locus_tag="ACHK74_000945"
     CDS             complement(48455..49384)
                     /gene="yhcC"
                     /locus_tag="ACHK74_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFP0219806.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            50059..54519
                     /gene="gltB"
                     /locus_tag="ACHK74_000946"
     CDS             50059..54519
                     /gene="gltB"
                     /locus_tag="ACHK74_000946"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFP0219807.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWEMGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDALAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            54532..55950
                     /gene="gltD"
                     /locus_tag="ACHK74_000947"
     CDS             54532..55950
                     /gene="gltD"
                     /locus_tag="ACHK74_000947"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFP0219808.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TNDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            56134..57261
                     /gene="yhcG"
                     /locus_tag="ACHK74_000948"
     CDS             56134..57261
                     /gene="yhcG"
                     /locus_tag="ACHK74_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFP0219809.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(57321..57785)
                     /gene="nanQ"
                     /locus_tag="ACHK74_000949"
     CDS             complement(57321..57785)
                     /gene="nanQ"
                     /locus_tag="ACHK74_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFP0219810.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(57782..58657)
                     /gene="nanK"
                     /locus_tag="ACHK74_000950"
     CDS             complement(57782..58657)
                     /gene="nanK"
                     /locus_tag="ACHK74_000950"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFP0219811.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(58654..59343)
                     /gene="nanE"
                     /locus_tag="ACHK74_000951"
     CDS             complement(58654..59343)
                     /gene="nanE"
                     /locus_tag="ACHK74_000951"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP0219812.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(59391..60881)
                     /gene="nanT"
                     /locus_tag="ACHK74_000952"
     CDS             complement(59391..60881)
                     /gene="nanT"
                     /locus_tag="ACHK74_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFP0219813.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYSSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(60990..61883)
                     /gene="nanA"
                     /locus_tag="ACHK74_000953"
     CDS             complement(60990..61883)
                     /gene="nanA"
                     /locus_tag="ACHK74_000953"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFP0219814.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(62005..62796)
                     /gene="nanR"
                     /locus_tag="ACHK74_000954"
     CDS             complement(62005..62796)
                     /gene="nanR"
                     /locus_tag="ACHK74_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFP0219815.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        15392 a        17104 c        15527 g        14871 t
ORIGIN      
        1 agcgcatcag gcattgtgta ggcagcagaa atgtcggata aggcaccgct gattactgat
       61 acaccggcag taaattaaag ctcgataaaa tatggaccag tgcgttgcca acgccaaaca
      121 ccagaatcag cgtaatcatc ggcttgccgc cccagacgcg gaatttcggg ctgccaaagc
      181 gtttacgcga tgcgcgggct aacagcgccg gaacaattgc cgcccagatg gtagctgcta
      241 aaccagcata accaatggcg tacaggaatc cgttcgggaa cagtagacca cccacaacag
      301 gcggggcaaa ggtcagcaat gccgttttca agcgccccag ggctgagtca tcgaaaccaa
      361 acagatctgc cagatagtca aacaaaccca gcgttacgcc aaggaacgaa ctcgctaccg
      421 caaagtttga gaacacgacc agcagaaggt ccaggctacg gctgttcagc acgccgctta
      481 atgcctgtac cagcacatca atattaccgc ccttctctgc aataccgata aactccggac
      541 gcgggatgtt acccatcgtc gccagcaacc agatggtata cagcgccagt gccatcagcg
      601 taccgtacac caggcatttc acgatggttt tcggatcttt gccgtaatac ttcatcaggc
      661 ttggcacgtt accgtgataa ccaaacgacg ccaggcagaa cggcaatgtc atcagcagat
      721 acggcgcata agatgcattg ctttctgcaa cgttgaacaa tgtcgcaggc tgcacatgcc
      781 ccaacaggct gccaaaggtg aggaagaagg taatgacttt cgcccccagc acaatcgccg
      841 tcatgcggct gacggcctta gtactcaacc acaccacaaa cgctaccagc agtgcaaaac
      901 caaaacccgc cgcccgtgcc gggacgttta gcgacatctc tgcgaaggtg tgatgcagaa
      961 tcgaaccact ggcagaaata taggcatagg tcaggatata gagcacaaag gcaatggaaa
     1021 taccgttgac cacgttccag cctttgccca gcaaatcttt ggtgatggtg tcaaaactcg
     1081 aaccgattcg gtaattcagg ttagcttcca gaatcatcaa gccggaatgc agcatacaga
     1141 accaggtaaa gatcagcgcc gccattgacc agaaaaacca cgccccggac atgaccactg
     1201 gcagagaaaa catccctgcg ccaataatgg tgccgccgat aatcaccacg ccgccaagca
     1261 gcgacggtga cgtttgggtg gtggttagtg ttgccatgag ggcttctctc cagtgaaaaa
     1321 tagtgcgact gcgttgttat gcattgcact gtaccagtac acgagtacaa aagacagaaa
     1381 aaaagccccg atggtaaaaa tcggggctgt atatattatt ttacagattg tgttcgctgt
     1441 tcagcgatga ttacgcatca ccaccgaaac gacgacgacc ggtagagtca tcacgacgcg
     1501 gagcgcggcc ttcacgacgt tcgccgctaa aacgacgacc atcaccacgg ccaccttcac
     1561 ggcgttcacc gctgaagtta cgaccgcctt cacgacgttc gccaccgaaa ccacgaccac
     1621 cgccacgacg ctcaccgcca gtatgcggct gtgcatcgcc cagtaactgc atgttcatcg
     1681 gcttgttgag aatgcgagtg cgcgtaaagt gttgcagcac ttcacccggc atacctttcg
     1741 gcagttcgat ggtggagtga gaagcaaaca gcttgatgtt accaatgtaa cggctgctga
     1801 tgtcgccttc gttagcaatc gcaccaacga tatgacgaac ttcaacacca tcatcgcggc
     1861 ccacttcaat gcggtacagc tgcatatcgc caacatcacg acgttcacga cgcggacgat
     1921 cttcacggtc accacgcggg ccacggtcgt tacgatcgcg cggaccacgg tcatcacggt
     1981 cacggaattc acgtttcgga cgcatcggcg catctggcgg tacgatcaga gtacgttcac
     2041 cctgtgccat tttcagcagt gccgcagcca gagtttcgag atccagctct tcaccttctg
     2101 cagtcggctg aattttgctc agcagtgcgc ggtattgatc cagatcgctg ctttccagct
     2161 gctgctgtac tttagcggcg aatttttcca gacggcgttt gcccagcagt tctgcgttcg
     2221 gcagttctac ttccggaata gtcagcttca tagtacgttc aatgttgcgc agcagacgac
     2281 gctcgcggtt ctcaacgaac agcagcgcgc ggccagcacg acccgcacga ccggtacgac
     2341 cgatacggtg aacgtaagac tcagaatcca tcggaatatc gtagttaact accaggctga
     2401 tacgctcaac gtccaggcca cgggctgcaa cgtcggtcgc aatcaggatg tccagacgac
     2461 catctttcag gcgttccagt gtctgttcac gaagcgcctg gttcatgtca ccgttcagcg
     2521 cggcgctgtt gtagccgtta cgctcaagag cttcagccac ttccagagtc gcgtttttgg
     2581 tacgaacgaa gataatcgcc gcatcaaaat cttccgcttc caggaaacgt accagtgctt
     2641 cgtttttgcg cataccccag acagtccagt agctctggct gatgtcagga cgggtagtca
     2701 cgctggactg aatgcgcact tcctgcggct ctttcataaa gcggcgggta atgcgacgaa
     2761 tcgcttccgg catggttgca gagaacagag cggtctgatg accttccggg atctgcgcca
     2821 taatggtttc aacgtcttcg atgaagccca tgcgcagcat ttcgtcagct tcatccagaa
     2881 ccagaccgct cagtttagag aggtccagag tgccacgttt caggtggtcc agcagacggc
     2941 ccggagtacc gacaacgatc tgcggcccct gacgcagggc gcgtaattgc acgtcataac
     3001 gctggccgcc gtacagagca accacattta cgccgcgcat gtgtttagag aaatccgtca
     3061 ttgcttcagc aacctgtacc gccagttcgc gggtcggtgc cagcaccaga atctgtggtg
     3121 ctttcagctc aggatcaaga ttctgcagca gaggtaaaga gaatgctgca gtttttccgc
     3181 tccccgtctg ggccataccc agaacgtcgc ggccattcag cagatgtgga atacactctg
     3241 cctgaattgg agatggtttt tcgtaaccca gatcgttaag ggcttcaagg ataggagcct
     3301 tcaggcccag atctgcaaaa gtggtttcga attcagccat gtagtacgtg tgcctcaaaa
     3361 ttaatggcgg ccagtctaca taactcatca tgaaattgat cagcaatttt cattgaaaag
     3421 tgtgaaccgg ctcaaagtag gtgtattaac gaacaacaac gccctcaccc gttaaggtga
     3481 tggcaatcaa aaaagattac gggctgatgt gtacgtcagc tattgctggt ccgattctgc
     3541 caggtcatct tggtcctggc ccaggagcga taattccaac aatgcgtatc ggtgctcaac
     3601 aaagttatga acgttgttgg caaccgccag tttgaacagt gccgtggcgc tgtccaaatc
     3661 ccccagactt aggtagtact tacctaaata gaagttggtt tcactgagat gctcagcgag
     3721 cgaggtgtta tccgttgcgt ccgccttgag cctttccatt aacgtttgtt cgctaatgtt
     3781 gcccaggtag aactcgacaa tgttccatcc ccactgttcc ttatccgatt tttcgaagtg
     3841 ctgtttcaac acttctttag cctgcttctc atcgagcttc tgctcggcga gataaagcca
     3901 cagactacgg aaaggatcat tgggatcgtc ttgataaaac gccagcagat catcttgcgc
     3961 taacttgtca cgaccgccgt aatataatgc gatcccgcga ttcaagtgcg cgtagttgta
     4021 agttggatca agctcaagta cagaatcaaa cgcttcatag gcagcatcaa aattgcctgc
     4081 ctgcgttaaa tatatgccta agtaattgaa tacttcaggc atatccggtc ggattgccag
     4141 cgcttgcgaa aaatcgttac gcgctaatgc cctcagaccg agactatcat acaacactcc
     4201 gcgctcatat aaaagctgtg cgcgttcgtc atcggttaaa gcccgactgg caaggatttg
     4261 ttccatacgt gccagaatca cttcctgctg taaagtcggt tgcaatggta ccgcgaggac
     4321 ttcactttta cgccaggaag tattactgca tcctgcaagc gtaagtgctg tcgcaacgaa
     4381 acaccagcgc aaaaaaggct tcatttccca ctcccgaaga ccacggttga atgaacgtcc
     4441 tgttcccggt tgctaacaag gcgtcctgcc cggttaaaag ccccccgccg cagcggaggg
     4501 caaatggcaa ccttactcgc cctgttcagc agccggagct tccggtgctg cagcaggttg
     4561 agactgctca gtcgcttctt taatgctcag acggatacgg ccctggcgat caacttccag
     4621 aactttcacc ggtacttcct gacccatctg caggtaatcg gtcactttct caacgcgttt
     4681 gtcagcgatt tgagagatgt gtaccagacc ttctttaccg ccgccgatgg caacaaatgc
     4741 gccaaagtca acgatacggg tcactttacc agtgtagacg cggcccactt cgatttctgc
     4801 agtgatctct tcgatacgac gaatagcgtg tttcgctttc tcgccgtcgg tcgctgcgat
     4861 cttcacagta ccgtcatctt cgatttcgat ggtggtgcca gtttcttcgg tcagggcacg
     4921 gataacagaa ccgcctttac cgataacgtc tttgatcttg tccgggttga tcttgatggt
     4981 atggatacgc ggtgcgaact cagagatatc gccacgcggc gcgttgatcg cctgttccat
     5041 tacgcccagg atatgcagac gcgcaccttt agcctggttc agcgcaacct gcatgatctc
     5101 tttggtgata ccttcaattt tgatatccat ctgcaatgca gagataccgt cgcgggaacc
     5161 cgcaactttg aagtccatat cgcccaggtg atcttcgtcg cccaaaatgt cagacagtac
     5221 aacgtagttg tcgccttctt tcaccagacc cattgcgata cccgcaacgg cagctttgat
     5281 cggcacacct gcgtccatca gcgccagaga cgcgccgcac acggaagcca tagaagagga
     5341 accgttggat tcagtgattt cagacacaac acgtacggtg tacgggaatt tgtccatatc
     5401 cggcatgact gccagcacgc cgcgcttcgc cagacgaccg tgaccaattt cacgacgctt
     5461 cggagaaccg accatgccgg tttcgcctac ggagtacgga gggaagttgt agtggaacag
     5521 gaaggtgtcg gtacgttcgc ccatcagttc atcaagaacc tgcgcgtcac gagcagtacc
     5581 cagcgttgcg gtaaccagcg cctgcgtttc accacgggtg aacagcgcag aaccgtgagt
     5641 acgcggcagc acgccagtac gcacatccag accacgaatc atatcttttt cacgaccgtc
     5701 gatacgcggt tcgcctgcca gtacgcggct acgaacaacg tttttctcga tagcgtgcag
     5761 aatttcaccc agttcgtttt cgtccagggt ttcgtcttca gcaagcagcg tcgcgatggt
     5821 ttcagatttg atgacatcaa cctgcgcata acgctcttgt ttgtcggtga tgcggtaagc
     5881 atcactcaga cgagcttcag ccagtgcagc aacgcgcgcg ttcagcgctt cgtttaccgg
     5941 ctccggctgc cagtcccaac gtggtttacc ggcttctttc accagttcat tgatgttctg
     6001 aataacaacc tgctgttgtt catgaccgaa cactactgcg cccagcatct ggtcttcgct
     6061 cagcaattca gcttcagatt caaccatcag tacagcggct tcagtaccag caacaaccag
     6121 atccagtttg ctctctttca gctcgtcctg agtcgggttc agtacgtact ggtcattgat
     6181 gtaacctacg cgggcagcac caatcggacc attgaacgga ataccagaca gagacagtgc
     6241 tgcggaagca ccaatcatcg cgacgatatc cggattaact tgcgggttaa cagaaaccac
     6301 ggtggcgata acctgaactt cgttgacgaa gccttccggg aacagcgggc gaatcgggcg
     6361 gtcaatcaga cgcgcgatca gggtttcgcc ttcgcttggg cggccttcac gacggaagaa
     6421 gctacccggg atacgaccag cagcgtaagt acgctcctga tagttaacgg tcagtgggaa
     6481 gaagtcctga cctggtttgg cttttttctg gccaacaacg gtaacgaata ccgcggtgtc
     6541 atccatgcta accataacag cggcagtagc ctgacgagcc atcatgccgg tttccagagt
     6601 cacggtgtgt tggccgtact ggaatttacg aacgatcgga ttaagcaatg taatatcctt
     6661 tctctttctt agacagtacc ttacggcact ggtgttaata cccgatcttc tgcgcatcct
     6721 cgcgactaat gacaacccta acccagctct atgtgggtaa agcctctcat tagccgcgcg
     6781 aacctctgca acggaagatc attcatagca acaatacatt agtttccagt gaattgctgc
     6841 cgtcagcttg aaaaaagggg ccactcaggc ccccttttct gaaactcgca agaattagcg
     6901 acgcagaccc aggcgctcga tgagctgggt gtaacgtgct acgtctttac gtttcaggta
     6961 gtcgagcagt ttacgacgct gagaaaccat gcgcagcaga ccacgacggc tgtggtgatc
     7021 ttttttgtgc tctgcaaagt ggccctgcag gtggttgatc tgtgcagtca gcagtgctac
     7081 ctgaacttcg gtagaaccgg tgtcgtttgc gtcacgacca aactcagaaa cgattttagc
     7141 tgttgcttca gtacttagag acattttaaa actccaaagt atatagaatg aaaggacgcc
     7201 gatctctaat tcagcgatcc cagtgtacgt tacgcaaagt gttaaacaat ttacgcgacg
     7261 ttaagcggca gtattctact cgtagcgacc tgttatcgca agacggttaa cattacgccg
     7321 ggtattcaac caccaggcga cgaggcgcaa cgcggccttc atcgtcaatt tcgcccatac
     7381 cgataaattt gccgttctca ccttctgtaa cgcgaaccag tccttccagc ggcgcaccgg
     7441 atgtacgaac tgggttacca tttttgaagt aaacagaaga cgttaacgga agattcacca
     7501 ccgggtagtc cgaagctgga ctgtccattg gcatcagtaa tggatcaagt aactccgcag
     7561 ccggaatatc ctgctgttcg gcttgttcga ccagttcacg cagatgttcc agggtcacca
     7621 tccgttcaac cggatattta cttaccgcca gacgacgcag gtaaataaca tgcgcgccac
     7681 aaccgagttt ttcacccagg tcatcaatga tggtgcggat ataagtgcct tttgagcagt
     7741 gaatttccag ctccagctca ttgccttcgt ggcgaataaa cagcaattca taaacggtaa
     7801 tcgggcgcgc ttcacgcggt acttcaatgc cctgacgcgc atattcgtac agttttttac
     7861 cctgatattt cagtgctgaa tacatcgaag ggatctgttc gatatcgcca cggaaagtat
     7921 ccagcgccgc cgccagttgc tctgcgctaa aggttaccgg acgttcttca acgatttgtc
     7981 cgtcggcatc agaagtatcg gtacgctgtc caagacgcgc aatgacccga tagcgtttgt
     8041 cggagtccag cagatactgg gaaaacttcg tcgcttcccc gaggcaaatt ggcaacatgc
     8101 cagttgccag cgggtccagc gcaccggtat gcccggcacg gttggcgtta taaatccgtt
     8161 tcactttttg cagcgcatca ttgctggaca taccctgcgg tttatccagc aacaaaacgc
     8221 cgttaatgtc gcgaccgcga cgacgaggac gactcattag tcctccttgc tgtcgtccgg
     8281 gttaacacga cgttcttcgt catgtttgac cacgctggtc accaggtttg acatgcgcat
     8341 cccttcaacc agagagttgt cgtagaagaa ggtcagttcc ggcacgatac gcaggcgcat
     8401 cgctttcccc agcaggctgc ggatgaaacc agaagcttct tgcaacgctt tgatgcccgc
     8461 tttaaccgcg tcttcatctt tgtcgttgag gaacgtcaca tatactttgg catacgccag
     8521 gtcgcgagac atttcgacac cggaaacggt ggtcatcatg cccaggcgag gatctttaat
     8581 ttcacgctgc aggatgagag cgatctcttt ttgcatttcc tgcgctacgc gctgcgggcg
     8641 accaaattct ttcgccataa taaattctcc tgacaaaaaa ggggctgtta gccccttttt
     8701 aaaattaatt tcaggtggaa gggctgttca cgttggcctg ataagacgcg ccagcgtcac
     8761 atcaggcaac ggctgccgga tgcggcgtaa aagccttatc cggcctacaa cccagacaac
     8821 cttaagcgat ggtacgttgg atttcgatga tctcgaatac ttcgatcaca tcgccagtgc
     8881 ggacatcgtt gtagttctta acgccgatac cacattccat accgttacgg acttcgttaa
     8941 cgtcatcttt gaagcggcgc agggactcca gctcgccttc gtagataacc acgttgtcgc
     9001 gcagaacgcg gatcgggttg tgacgtttaa ccacaccttc ggtaaccata caacctgcaa
     9061 tggcaccaaa tttcggtgat ttgaatacgt cacgaacttc cgccagaccg ataatctgtt
     9121 gtttcagttc cggagacagc ataccgctca ttgccgcttt cacttcgtcg atcaggttat
     9181 agatgacgga gtagtaacgc agatccaggc tttccgcttc aatcacttta cgtgcagagg
     9241 catcagcacg tacattaaag ccaaccagga tggcgttgga cgccgcagcc agggtggcgt
     9301 cggtttcggt gataccacct acgccagaac cgatgatctt cactttaact tcgtcagtag
     9361 acagtttcag caaggagtcg gagatcgctt ccacagaacc ctgtacgtct gccttcagga
     9421 caatgttcac ttcgtgaact tcaccttcgg tcatgttggc gaacatgttc tcgagtttag
     9481 atttctgctg acgcgccagt ttaacttcgc ggaatttacc ctgacgatac agtgcaactt
     9541 cacgcgcttt cttctcgtca cgtacaacgg taacttcatc acccgcagcc ggtacgccgg
     9601 acaggccgag gatttccacc ggaatggacg gacccgcttc cagcacttcc tgacccagtt
     9661 cgttacgcat cgcacgaaca cgaccgtatt cgaagccaca cagaacgata tcgcccttgt
     9721 gcagagtacc ttcacgtacc agaacggtag caaccggacc acgaccttta tcgaggaagg
     9781 attcgataac cgcaccgctc gccatacctt tacgtaccgc tttcagctcc agaacttccg
     9841 cctgcagcag gatagcgtcc agcagttcat cgatgccggt acccgctttc gcagatacgt
     9901 gtacgaactg gctttcaccg ccccactctt ccggcaggat gccatactgg gagagttcgt
     9961 tcttaacacg atccggatcg gcttctggtt tatcaatctt gttcactgca accaccaccg
    10021 gtacctgcgc cgctttcgcg tgctggattg cttcgatggt ctgcggcatc acgccgtcgt
    10081 cggcagcaac aaccaggact acgatgtccg ttgcctgcgc accacgtgca cgcattgaag
    10141 taaacgcggc gtgccccggg gtgtccagga aggtgatcat gccgttttca gtttcaacgt
    10201 ggtatgcacc aatgtgctgg gtaatgccgc ccgcttcgcc agaggccact ttcgttgaac
    10261 gaatgtagtc cagcagagag gttttaccgt ggtcaacgtg acccatgatg gtcacaaccg
    10321 gcgcgcgcgg ttcagccgca gcccccgtgt cacggtcgct cattaccgcc tcttccagct
    10381 cgttttcacg acgcaggata actttgtggc ccatctcttc agcaaccagc tgtgcggttt
    10441 cctgatcgat aacctggttg atggttgcca ttgcgcccag tttcatcatc gctttgatga
    10501 cctgagagcc tttaaccgcc atcttgttcg ccagttcgcc aacggtgatg gtttcgccga
    10561 tcacaacgtc acggttaacg gcctgagctg gcttctggaa gccttgctgc agggaagaac
    10621 ctttacgttt tccgccttta ccgccacgta ctgctgcgcg agcttcttca cgatcagctt
    10681 ttgattcagc gtgtttgttg cctttcttcg gacgcgctgc tttcgcgtta cgaccacggc
    10741 cacggccgcc ttcgacttca cgatcgcttt cgtcttctgc ctggcgagca tgttgagaag
    10801 tagtgacgtg ataatcgctg gaatcttcag tcggttccgc gttatcagtc catttgtttt
    10861 cttccgccat acgacgtgct tcttcagcaa cgcgacgtgc ttcttcttcg agtttacgac
    10921 gcgcttcttc ttcagcttta cgcttgagct ctgcagcttc ctgctcacgg cgggcttttt
    10981 cagcctgggc gtttttagtc atatcgtctt gttgattgct cactttgtct ttttccgcag
    11041 cttcacgttt cgcttgttca gcagcttcac gcttagcttg ttctgcggcc tcacgttcag
    11101 ctttttgttg cgcctcgcgt ttagccgatt cttctgcctc acgacgggct tgctcttccg
    11161 cttcacgctg cgcttgctct tccgctgcaa ggcgttcagc ctcttgcgga tcgcgtttca
    11221 caaaggtgcg tttcttgcgg acttcgattt gtaccgattt gctttttcca ccggtaccag
    11281 gaatgttaag ggtgctgcgt gttttacgtt gcagcgtcaa tttgtccggg cctgaatttt
    11341 tctgattcag gtggtcaatc aaagtctgtt tctcttgtgc ggacacagag tcgtcagcag
    11401 acttccggat acctgcatca gcaaattgct gtaccaggcg ttccacggag gtctgtcgct
    11461 ctgcggccag cgttttaatc gttacatctg tcatgctgtt ccttcctgct acagtttatt
    11521 acgcttcgtc accgaaccag caaatattac gggcagccat aatcagtgct ccggcttttt
    11581 cgtcggtcaa cccttcgata tcagccagat catcaatgcc ctgttcggcg agatcttcca
    11641 gcgtacaaac gccacgggcg gccagtttga atgccaaatc acgatctatc ccttcaaggt
    11701 tcagcagatc gtcagccggt ttgttatcac cgaggctttc ttcctgggcc tgtgcaatgg
    11761 tggccagtgc atttttagca cgctcgcgca gtgcttcaac ggtcggctca tcaaggcctt
    11821 cgatttccaa cagctctttc atcggcacat aggccagttc ttccagcgtc gagaagcctt
    11881 cttctaccag aacagtcgcg aagtcttcgt cgatgtcgag atatttggtg aaggtgtcga
    11941 tcgctgcgtg cgcttccgcc tgatgcttag cctgcaggtc gtcaacggtc atcacgttga
    12001 gttcccaacc gctcagctgc gaagccagac gcacgttctg accgttacgg ccaatcgcct
    12061 gcgccaggtt accggcttca acggcgatat ccatggtgtg tttatcttca tccaccacga
    12121 tagaagcaac gtctgccggt gccattgcgt taatcacaaa ctgcgccggg ttatcatccc
    12181 acaggacgat atcgatacgc tcgccgccca gttcagtaga aaccgcctga acacgcgcgc
    12241 cacgcatacc tacacaggca cctaccggat cgatacgttt atcgttggtt ttcaccgcga
    12301 ttttcgcacg agaacccgga tcgcgagccg ctgctttaat ttcaatcact tcttcgccga
    12361 tttctggcac ttcaatacgg aacagttcga tcagcatttc cggcttggaa cgagtgacga
    12421 acagttgcgc gccacgcgct tccgggcgaa cggaatagag cacgccacga acgcggtcgc
    12481 cagggcggaa gttttcacgc ggcagcatat cttcgcgcag gatcacggct tcagcattgt
    12541 tgcccagatc cagagagatg ttgtcgcggt ttactttttt caccacgccg gtgatgattt
    12601 caccttcgtg ttcacggaac tgatcaacca ccatcgcacg ttcggcttca cgcactttct
    12661 gcacgataac ctgttttgcc gtctgggtag tgatacggtc aaaggtaaca gactcaatct
    12721 gatcttcaac gtaatcgccc aggttcaggc tttcatcttc ataacgtgcg gcttcaaggg
    12781 tgatttcctt ggtcggctgg gtgacttcat caacaactaa ccagcgacgg aaggtgtcaa
    12841 aatcaccgct tttgcgatcg atctgtacgc ggacgtcgat ctcttgttca tattttttct
    12901 ttgttgctgt cgccagcgcg ctttccaatg cttcgaaaat cttctcgcga ggtagcgcct
    12961 tttcattgga tacggcttca actacagcca aaatttcttt gttcatcgcg ggcttttcac
    13021 ctcatccaga ctattaaaag tggggaacca ggttcgcctt ctggatatta ctcagcgcga
    13081 acacttcatc tttaccttcg acggtaactg tgatcatttc accgtctacc gctttgataa
    13141 cgccctgcca tttacgacgg ttttgtaccg ccatacggag aaccagagtc acctcttctc
    13201 cgacaaaacg ggcgtagtgt tcagccgtga acagtgggcg atcgagaccc ggtgaggaga
    13261 cttccaggtt ataagcaacg gtgatgggat cttcaacatc cagcacagca cttacctggt
    13321 ggctcacatc agcacaatca tcaacattga tgccatcttc actatcaata tagatgcgca
    13381 gtgtggatgt gcgaccgcga ataaattcga tgccaaccag ttcaaaaccc agggcctcaa
    13441 ctggcgcagt aatcatctct gttaattttt gctctaatgt ggacaagccc acccccaaga
    13501 cataaaaaaa gggcctaaag cccagttatt ctgtagtcag ataacaaaaa accccgataa
    13561 atcggggctt tatataactg aaccctataa ccgcaactgc ggtctggagc actttccaga
    13621 aggatttttt caaatcccac tacgaaggcc gaagtcttca cagtatattt gaaaaaggac
    13681 tctaagggaa agtggttgcg ggggccggat ttgaaccgac gaccttcggg ttatgagccc
    13741 gacgagctac caggctgctc caccccgcgc ctgaaacgtg gcaaattcta ctcgttttgg
    13801 gtaaaagatg caaatactgc tgggatttgg tgtaccgaga cgggacgtaa aatctgcagg
    13861 cattatagtg atccatgcca cattttgtca acgtttattg ctaataacgt gaatgaatat
    13921 ccagttcact ttcatttgtt gaatactttt gccttctcct gctctccctt aagcgcatta
    13981 ttttacaaaa aacacactaa actcttcctg tctccgataa aagatgatta aatgaaaact
    14041 catttatttt gcataaaaat tcagtgaaag cggaaatcca ggctcatcat cagttaatta
    14101 agcagggtgt tattttatga cgacgattct caagcatctc ccggtaggtc aacgtattgg
    14161 tatcgctttt tccggcggtc tggacaccag tgccgcactg ctgtggatgc gacaaaaggg
    14221 agcggttcct tatgcatata ctgcaaacct gggccagcca gacgaagagg attatgatgc
    14281 gatccctcgt cgtgccatgg aatacggcgc ggagaacgca cgtctgatcg actgccgcaa
    14341 acaactggtg gccgaaggta ttgccgctat tcagtgtggc gcatttcata acaccaccgg
    14401 cggcctgacc tatttcaaca cgacgccgct gggccgcgcc gtgactggta ccatgctggt
    14461 tgctgcgatg aaagaagatg gcgtgaatat ctggggtgac ggtagcacct ataaaggaaa
    14521 cgatatcgaa cgtttctatc gttatggtct gctgaccaat gctgaactgc agatttacaa
    14581 accgtggctt gatactgact ttattgatga actgggcggc cgtcatgaga tgtctgaatt
    14641 tatgattgcc tgcggtttcg actacaaaat gtctgtcgaa aaagcctact ccacagactc
    14701 caacatgctt ggtgcaacgc atgaagcgaa ggatctggaa tacctcaact ccagcgtcaa
    14761 aatcgtcaac ccgattatgg gcgtgaaatt ctgggatgag agcgtgaaga tcccggcaga
    14821 agaagtcaca gtacgctttg agcaaggtca tccggtagcg ctgaacggta aaacctttag
    14881 cgacgacgta gaaatgatgc tggaagctaa ccgcatcggc ggtcgtcacg gcctgggcat
    14941 gagcgaccag attgaaaacc gtatcatcga agcgaaaagc cgtggtattt acgaggctcc
    15001 ggggatggca ctgctacata tcgcttatga acgtctgttg accggtattc acaacgaaga
    15061 caccattgag cagtatcatg cacatggtcg tcagttgggc cgtctgctgt accaggggcg
    15121 ttggtttgac tcccaggcgc tgatgctgcg tgactctctg caacgctggg ttgccagcca
    15181 gatcactggt gaagttaccc tggagctgcg ccgtgggaac gattattcaa tcctgaatac
    15241 cgtctcagag aacctgacct acaagccaga gcgtctgacg atggaaaaag gcgactcggt
    15301 gttctcgcca gatgatcgta ttggtcaatt gaccatgcgt aatctggata tcactgatac
    15361 ccgagagaaa ctttttggtt atgccaaaac tggcctgctc tcctcctctg ccacttcagg
    15421 cgtgccgcag gtggagaata tggaaaacaa aggccagtaa ttcgacatca accctgccct
    15481 tcaggggtgg ggttaccttg cttatcctct gtaaactcct ttctgagcat cacataatcg
    15541 aaaacatcat ggtttgcatc cacaacccgt gagtctcctt tatagtgtgc aatcacttcc
    15601 tcaagcgttt gcggctgttc cggctttgtt acccccatcc acgcattaat aaggtaatta
    15661 ttgtaagctg tcgaaaagat gttgttttcc gttgtgcgat ccacgccatc cccaagaacg
    15721 gggctatacc agataaacat cgggacatga tatgcctgct ggctagcctc cctgcctcca
    15781 tgaaaataga cgttcttctt cgtagggtca cgttccaggc cgtggtcggc aaaatacatg
    15841 accgaggcgc ggcgatcttt taataattca aaaacctgac ctagcaaact atctgtgtaa
    15901 tgaatggagt tgtcatagca ggcatcctga tcgtcctgcg gttgaaacac ggcgctggat
    15961 tgcggatagg cgctacaagc cggttcatgg cttccattta aatgaagaac aatcagtttc
    16021 ttttgctgcg tattttgctg taatgcttgc gataaatgcg gcaacaacaa ttcatcaaat
    16081 cctctgacat aaactgtttc catggcgcgc atggcgatac tggtaactgc tgtaccattc
    16141 tgccgaaaag cggattgcga gcttagccag aaagtctgaa atcctgcttg attagccata
    16201 ttaataatgt tgtcggggta attatgaatg tcatgactca aaacagaatc agcagttaaa
    16261 gaaaggggaa ccgacagcgc agtgtaaggt gcgccgctta ttgcttgatt aaacagtttg
    16321 atctgttttc tttgtgcttc aacttgcggt gtcgtagagc gtgtatatcc atacaaagac
    16381 atattgtcga cacgtacaga ctcccccaca atcaacacgt aggtatcaat acctgtatcc
    16441 ctgactgata attgaaaata cggcaccgtg tttgcaattg agagtaatct ttgatgctct
    16501 ttcgctgcta aagcaaaata gttgagattg aaaaacggcg tataggtagc aaatcgcgac
    16561 gctagtatat atggactgaa cgctttttta ttttttgcat ctttataagc aaattgacaa
    16621 gcggaaaata aactgcccga aatgacaatc agcaataata ttcctgtcac tttttttgtc
    16681 ggcaaggaaa catcatattt tattattact gccaaaaaaa gaagggataa aaaagcaaag
    16741 gcacatagat aagggatata catccccagc attttgacaa cttcatccgg atcactctgg
    16801 agcacactaa tcgcaaatcc atcattgaaa gttgtaccaa aggtacacca tgaatagagg
    16861 ctaatactca tgtctgccgc agtcagtaac acaaatggtg cggcaacaag caggcgaagc
    16921 ggaagggatg atatacgttt tacaaccagg atggtcagga cgaagaacag caatcgctga
    16981 accatatgcg cgccggaaga ggcgactaag ttcgtaatac caaaaacaat aacacaaaga
    17041 tacaacaacc aatgagattt aaaacttcta gcaaatttgt tgaatactgt catccagaaa
    17101 tccaagttca caaccaatat aaacaattat caacaatgtt caattttata tcagacatgt
    17161 ttttcaactc tgatccatat cagaccgaaa cactgttagt tttttctttt taaatgatgc
    17221 ggccattgcg ttctcagtaa ttaaataaca ttatcaatgc gtctctaaac attacgcaga
    17281 acgtagccat cagtagttat actgacagga gcaggatggt ttatcgagaa gtaaacatat
    17341 ccgtattcgt accggaactg gagttgacac aataaagtgc caattatgtc agcagaaggg
    17401 aaaagtttat aactaaggcg taccggcacc atcgtttcaa ggtaccagct acgagtaaag
    17461 caactggacg agatacagat acctgacaac cattcctcag accaggacca aaacgaaaaa
    17521 agacgctttt cagcgtctct tttctggaat ttggtaccga ggacgggact tgaacccgta
    17581 agccctattg ggcactacca cctcaaggta gcgtgtctac caattccacc acctcggcac
    17641 ggatactact ttttagttcg ggatatcgct ggtcggctta gccggagcag ctggttgagt
    17701 ttgttcggtt ttcaccggtg cactcagatt ttcccattcg ctacctttat tggttttgtt
    17761 gctattgatg ttacccagca ccagactgat gatgaagaat aacgttgcca gcagcgccgt
    17821 catgcgggtc atgaagttac cagaaccact tgaaccaaac agcgtagcgg aagcgcctgc
    17881 tccgaaggag gctcccatat cagcgccttt accttgctgc agcatgatca gaccaacaag
    17941 gccaattgcc acaataagga aaactactaa aagagcttcg tacataatca acctgttcct
    18001 tgcggagttg ccgcgtacca atgcttcaac caataaagcg ggagttttta atctttccca
    18061 ctgaagcggg tgtgaatact aaccaaagcg aatgaccttc gcaagggcaa ttttatcgca
    18121 ttgtatcaac tgcggaaaaa aacagcaaaa ccgatgtgtt cgctgagaaa aaaggcgaca
    18181 atcgccgcct ttttagccag ttatctaacg ctttaaacgg cttttactgc atcggcgatg
    18241 cggtgtgcaa attcagtcac ctgcgcttcg tcttcgcctt ccaccatcac gcgaattaac
    18301 ggttcggtgc cggatttacg cagcaacacg cgtccacggt tgcccagcgc agcttcaacc
    18361 tctgcggtca cggctttaac tgactcatgc tcaagtggat cgccgctacc tgcggtgtaa
    18421 cgtacgttaa ccagaatctg cgggaacatt ttcatgccgc tgcaaaggtc gtgcaggctc
    18481 atatggttac gtgccatcgc cgccagcacc tgcaagccag caacgatgcc gtcaccggta
    18541 gtagttttat ccagtaggat cacatgaccg gaattctctg caccgatacg ccagcctttc
    18601 tcctgcattt tttccagtac gtagcggtca cccactttcg cgcgcgcaaa tggaattcct
    18661 aactgtttca gcgccagttc aagccccatg ttgctcatca atgtacccac agcgccacca
    18721 cgcagctggc cctgacgaag accttcacgc gcgatgatat acatgatctg atcgccatcg
    18781 actttattgc cttcatggtc gaccataatc acgcgatcgc catcgccgtc gaaggcaata
    18841 ccgagatccg ctttttcagc cagcacacga gcctggagcg cgcgaacgtc ggtagccccc
    18901 acttcggcat tgatgtttac accgtttggc tcacaaccga tagcgataac gttcgccccc
    18961 agttcgcgca gcacgttcgg cgcgatgtga taagtcgcac cgtttgcaca atccaccaca
    19021 atcttcagtt cactgaggct aagttcgttc gggaacgtgg ctttgcaaaa ctcgatatag
    19081 cgacccgcgg catcaacgat acggctggct ttacccagtt ctgccgaatc aacgcagctg
    19141 atctcctttt ccatttccgc ttcgatggcc tcttctaccg catccggcag tttggtgccg
    19201 tcgatagaga agaatttaat cccattatcg tagaacgggt tatgcgatgc agatatcaca
    19261 attccggcct ctgcgcggaa ggtacgcgtc agataagcca cggccggtgt tggcatcggg
    19321 ccagtgaaga gtgcggaaag gcccgctgcc gccagacccg cttccagtgc tgactccagc
    19381 atatagccag aaatacgcgt gtctttacca ataataatct tacgggagcc gtggcgcgcc
    19441 agcactttac ccgcggccca acccagctta agcacaaaat caggtgtgat cggcgcatcc
    19501 cctacacgac cacgaatccc atcggtaccg aaatatttac gattactcat agcgtttgtt
    19561 ttcctttgca gacagagtgg cttccaccac ccgcatcgct tctacggttt ctttgacgtc
    19621 atgaacacga atgatgtgcg cgccttgcat tgcggcaatg accgcacagg ccagactacc
    19681 gctcaggcgc tcggacggcc ccacgttcag cagctgccca atcatcgatt ttcgtgacat
    19741 acccaccaac agcggcaggt tgaaatggtg aaattcagcc aggcgcgcca gtaatgaata
    19801 gttatgggag agatttttac cgaaaccgaa tccggggtcg agcaacaatt tctcttttgc
    19861 gatacccgcc tgctcgcaac gtgctatttg ctcaataaag tagcgattca cttctgcaaa
    19921 gacatcgtca tacttcggag cttcctgcat ggtttttgga tttccctgca tatgcatcag
    19981 acaaaccggt aaaccggttt ctgcagccgc ctccagagcg ccaggttcgg aaagggagcg
    20041 gatatcatta ataatgtgag cgccaacttt cgctgactca cggatgactt ctggtttgga
    20101 tgtatcgacc gagatccaga cttcgaagcg ttgagcaatt gcctcaacca caggaataac
    20161 acgttgcaac tcttcttcaa cgctaacttc cgccgcccct gggcgcgtgg actcgccacc
    20221 aacgtcaatg atcgtcgcgc cagcattgat catcagattc gcatgtttca ccgcatctat
    20281 cagcgagtta tgcgtgccac catccgaaaa ggaatcaggc gtgacgttga ggatccccat
    20341 tacgtgagga tggctaaggt ccagtgaagt accctgggca aagagtttca tgatgttatc
    20401 cctggtatga attgataaga aaaaaccccg gagcacgccc cggggttttc ggtacaaata
    20461 cagtcatctg atgcgggaac ttacttgtcg cctaactgct ctgacatggt gttacccggg
    20521 ttcggcgtac gcggttcatc aaccggacga ggagcctttg gactaccatt gtcgccagaa
    20581 ttgttagaag cgcctggttc ttcccagccc gctggcggac gtacatcgcg acgtgccatc
    20641 aggtcatcaa tctgcggtgc gtcgatagtc tcatatttca tgagagcatc tttcatcgca
    20701 tgcagaatat ccatattgtc ggtcagaagc tgacgcgcac gattatagtt acgctcaatc
    20761 agtgctttca cttcctggtc gatgatacgt gcagtttcat cggacatatg tttcgctttc
    20821 gctacgctac ggccgaggaa tacttcacct tcttcttccg cgtacagcag tggacccaat
    20881 ttctcagaga agccccactg ggtcaccatg ttacgtgcca gattggtcgc aactttaata
    20941 tcgttggacg caccggtaga aacatgttcc ggcccataga tgatctcttc tgccagacga
    21001 ccaccgtaca gcgtagaaat ctggctttcc agtttctgac ggctggcgct gattgcgtcg
    21061 ccctcaggca agaagaaagt cacacccagc gcacgaccgc gtgggataat cgtcactttg
    21121 tgcaccggat cgtgttccgg caccaggcga ccgataatcg catgacccgc ttcgtggtaa
    21181 gccgttgatt ctttctgcgc ttccgtcatc accatggagc gacgttccgc acccatcatg
    21241 attttgtctt tcgctttctc gaactcaacc atcgacacaa cgcgtttgtt gccacgagca
    21301 gcgaacagtg ccgcttcgtt caccaggttc gccaggtcag caccggagaa accaggagta
    21361 ccacgggcaa tgattgccgc gtcgatatcg ggggccaatg gtacgcgacg catgtgaact
    21421 ttcaggatct gctcacgacc gcgaacatct ggcaagccga ccacaacctg acggtcgaaa
    21481 cggccaggac gcagcagggc cgggtcgaga acgtccggac ggttagtcgc ggcgataacg
    21541 atgatacctt cgttaccttc gaagccatcc atctcaacca gcatctggtt cagagtctgt
    21601 tcacgttcat cgtgaccacc gcccagacca gcgccacgct ggcggcctac ggcgtcgatt
    21661 tcatcgataa agatgatgca cggtgccgct ttcttcgcct gttcgaacat gtcacgaaca
    21721 cgggatgcac ccacaccgac gaacatttct acgaagtcag aaccggagat agtaaagaac
    21781 ggaactttcg cttcgcctgc aatcgctttc gccagcaacg ttttaccggt acccggagga
    21841 ccgaccatca ggacgccttt cgggatctta ccgccgagtt tctggaagcg gctcggctcg
    21901 cgcagatact ctaccagttc agcaacttct tcttttgctt cgtcgcagcc cgcaacgtca
    21961 gcaaaggtcg ttttgatctg atcttccgtc agcatgcgcg ctttgctctt accaaacgac
    22021 atggcacctt tgccaccgcc gccctgcatt tgacgcatga agaagatcca gacaccaatc
    22081 agcaacagca tcgggaacca ggagatgaag atagaagcca gcaggcttgg ttcttcaggc
    22141 ggttcaccga caaccttgac gttcttggtc aacaggttat ccagtaattt cggatcctga
    22201 accggaatgt aagtggtata acggttacta tctttcttgg taacgttgat ttcacgtccg
    22261 ttgatacgcg cttcacgaac ctggtcgtta ttgacctctt gtaggaaggt agagtaatcc
    22321 accttacggc cattagactc gctgggccca aagctctgga atactgacat cagcacaacg
    22381 gcaatgacca gccagagtat taggtttttc gccatgtcac tcaagggatt aacctcttat
    22441 tacaactgtg ttaaaaacag cgtcaggata ctctatatcc agcatctttc aaactttcgt
    22501 ctgaaatctc ccggttaggg tttacgcccg gtcgctacaa tatacacttc ccgcgaacgt
    22561 gcacgagaag agtccggctt acgaactttg accttcgtaa acagggagcg aatttccctt
    22621 agatactcat cgaaaccttc gccctggaac accttcacta caaaactgcc acctggcgct
    22681 aatacatcac gacacatttc taacgccagt tccaccagat acatggcacg ggggatatcc
    22741 accgccggtg ttccgctcat gtttggtgcc atatcggaca tgacaacctg gactttgctg
    22801 tcgccaacgc gctccagcag tgctttcatc accagttcat cacgaaaatc gccctgaaga
    22861 aagtccacac caacgatagg atccataggt aaaagatcgc aagcgatgat gcggcctttg
    22921 ccgccaattt gggtgaccac atattgtgac caaccacccg gagcagcacc aaggtcgaca
    22981 accgtcattc ccggtttaaa gagtttgtca ctttgctgta tttcatcaag tttaaaccag
    23041 gcacgggaac gtaacccctt tttctgtgcc tgttgaacat atttatcgct aaagtgttcc
    23101 tgaagccagc ggctggagct ggcagaacgc ttcttacctg tcatttaact ttcccatggg
    23161 gataactcat cgtaaccaat tgcgtaaatt tttacgcgcc tatttggtga tatatgggag
    23221 atggcggtag aatgacccgt tttcaatccc aacgtaagca aaaaatacga tgaatctgag
    23281 tactaaacaa aaacagcacc tgaaaggtct ggcacatccg ctcaagccag ttgttctgct
    23341 tggcagtaat ggtttgaccg aaggggtgct ggccgagatt gaacaagcgt tagagcacca
    23401 tgaactcatc aaggtgaaaa tcgccaccga agatcgcgaa actaaaacct tgatcgtgga
    23461 agctatcgtg cgcgaaaccg gcgcctgtaa tgtacaggtc atcggtaaaa cgctggtgct
    23521 ttatcgccca actaaagaac gtaaaatctc gctgccacgc taagattatc ctaaagttac
    23581 acacatttgc tgtgtaaaac gaggggtttt ccgcaggcag gagagcaaaa tgccacgctc
    23641 tgttcgttga taaaaggccg catagcggcc tttttccttt ctttacaata catcaacatc
    23701 ttgagtattg ggtaattctt acaggtattc caccttaatt acttcaaatt ctacttcgcc
    23761 gcccggcgtt ttgatgacca caacatcatc ttcttctttg ccgatcaggc cacgagcaat
    23821 aggcgagttt acagaaatca ggttttgttt aaagtcagct tcgtcatcgc caacgatgcg
    23881 ataagtctgt tcttcgtcag aatccagatt cagcaccgtt acggtagcac caaaaataac
    23941 gcgcccattg ttgggcattt tggtgacatc aatcacctgc gcgttcgaca gcttggcttc
    24001 gatgtcttta atacggcctt cgcagaaacc ctgctgttca cgagctgcgt ggtattcggc
    24061 gttttctttc aggtcgccat gctcacgcgc ttccgcgata gcagcaatga tttcaggacg
    24121 gcgcacagat ttcagaaaat ccagctcttc gcgtaatttt tcagcgccgc gtaaggtcat
    24181 cggaatagct tgcatttgtt atacctcttg aatattcctg atggggcaag tcttcaccca
    24241 ccccggctgt tcggcctgcc cggcataact cctcaccggg accagaagca aaaaaatacc
    24301 gacccgggta caagtcccag gtcagctaca attcacattt tgatagtcat tttaccctga
    24361 agttcccgaa gggtcatcgt ttactttata gggcgttgcg ccgtagtatg acggctcgat
    24421 tccaggttgt tagcgcgaga ttatgcgatt ttccagattt atcatcggat tgaccagctg
    24481 tatagcgttc agtgttcagg ccgcgaatgt tgatgagtac gttactcaac tccccgctgg
    24541 tgccaacctt gccctgatgg tgcaaaaagt cggcgcgtcg gcccccgcta ttgattacca
    24601 cagtcagcag atggcgctgc ccgccagtac gcagaaagtg attactgcgc tggcggcgtt
    24661 gattcaactc ggccccgatt ttcgtttcac cacgacgctt gaaaccaaag gcaatgtgga
    24721 aaacggcgta cttaagggtg acttagtggc gcgatttggt gccgatccga cgttaaaacg
    24781 tcaggatatt cgcaatatgg tcgcgacttt gaaaaaatct ggcgtcaacc aaatcgatgg
    24841 caatgtgttg atagatacct ccattttggc cagccacgat aaagcccccg gctggccatg
    24901 gaatgacatg acacaatgct ttagcgctcc gcctgccgct gccatagttg accgcaactg
    24961 tttctccgtc tcgctctaca gtgccccaaa acctggtgat atggctttta tacgcgtggc
    25021 atcttattac cccgttacga tgttcagcca ggtacgcacc ctcccccgag gttctgccga
    25081 agcgcaatac tgcgaactgg atgtcgtacc aggcgacctg aaccgcttta cgctgacggg
    25141 atgcctgcca caacgttctg agccgctccc gttggctttt gccgtgcagg atggagccag
    25201 ctatgccggg gcaattctga aagatgagtt aaaacaggca ggtatcacct ggagcggaac
    25261 actgctgcgc cagactcagg ttaacgaacc tggaacggta gttgccagta aacagtcggc
    25321 cccgttgcac gatctgctta agattatgct gaaaaagtcg gacaacatga tcgccgatac
    25381 ggttttccgc atgataggcc atgcgcgctt caatgtgcct ggaacatggc gggccgggtc
    25441 ggacgccgtg cgtcagatcc tgcgccagca agccggtgtc gatattggaa acaccattat
    25501 tgccgatggt tcagggcttt cgcggcataa cctgattgcc cccgccacca tgatgcaggt
    25561 gctgcaatac attgcccaac acgacaatga acttaacttt atctccatgc tgccgcttgc
    25621 gggctatgac ggctctttgc agtaccgtgc aggtctgcat caggcgggcg tggatggaaa
    25681 agtctcagcg aaaaccggtt cgttgcaggg ggtatataac ctggcgggat tcattaccac
    25741 agcgagcggg caacgaatgg cgtttgtaca atatctttct ggctatgcag tagaaccggc
    25801 ggatcagcgt aatcgccgta ttccgttagt gcgttttgaa agccgtttgt ataaagatat
    25861 ttatcagaac aattagtcaa aaagaaaccc cggcacatgg ccggggtttt gctgattaac
    25921 gcttgtaaat gaactcaacg ccttcttcgt cgtcttcgtc ccagtcatca tcccagtcct
    25981 catcatcctc ttcagcaatc tcttcaagct gctggcgatg ataatcatcc cacatgaatt
    26041 cgactttctc tggctgtttc gcttcttcag cctgcacgac cgggttttca atgataaagg
    26101 tcatcacatc ccagcagaga tctttcacgc ccagtccact cgccgcagag atcagataat
    26161 atttatcttc ccagcccagc gcttcagcaa tcgctttcgc tttctcttca gcttctgcct
    26221 tatccagcag atcgatctta ttgaacacta accaacgtgg tttcgccgcc agatcctggc
    26281 tgtatttttc cagctcgctg ataataatac gcgcgttttc aaccggatcg gtgccgtcaa
    26341 tcggatcgat atcgatgagg tgcaacagga cgcggcaacg ttccaggtgc ttcaggaagc
    26401 gaatgcccag acctgcgcct tccgcagcgc cttcaatcag tcctggaata tcggcaacaa
    26461 cgaagctctt ttcgttgtcc attcgtacca cacccagact tggcaccaga gtggtaaacg
    26521 gataatccgc cactttcggt ttagccgccg ataccgcacg aataaaggtc gatttacccg
    26581 cgtttggcat ccccaacata ccgacgtcag ccagcagcat cagctccagc agcagctcac
    26641 gcttatcgcc cggcgtaccg ttagttttct gccgtggtgt acggttaact gacgatttga
    26701 aacgggtatt gcccagaccg tgccagccgc ccttagcaac cagcagacgc tgaccgtgtt
    26761 tggtcatatc gcccatggtt tcaccagtac cctggtcgat tacacgcgta cctaccggca
    26821 ctttaattgt gacgtcttta ccgcgcttac cggtacagtc gcggcttgcg ccattctgac
    26881 cgcgctctgc acggaaagat ttttcaaaac gataatcgat aagcgtgttc aggttctcgt
    26941 cggcttccat ccatacgtca ccaccatcac cgccgtcgcc gccatccggg ccgcctttcg
    27001 gaatatactt ttcgcggcgg aagctcacgc aaccattacc gccatcacct gcaacgacca
    27061 gaatcgatgc ttcatcaaca aacttcattt tattctccgt aaatcattcg cctgcgcggg
    27121 gttgcgatac caccgttgta tgcttacgta atccgcccca aatacgatga ccaatggcgg
    27181 aatacatcgc gcccgcaacc acgacaaacg caccgagata acctaaaagg tttaacatcg
    27241 gtctggcgaa gaaatcgggc caggccagtg ataaaagatc tgaaaaaaac agcgtaaaca
    27301 gtggggtgag cgtgatgatc gcgctcacct gcgctgcctg ccagcgagcc atcgcttccg
    27361 ccagggcgcc atatcctacc aaggtattca gtccgcaaaa aattaaacat gcgagctgcc
    27421 agtggctaag ctgcgctatc actccaggct ttgccagagg gaagagcgca attgtacata
    27481 aagtgtacag taaaaacagg atctgcggtg aggccagccg acgcaataaa accttttgcg
    27541 ccacgccata actcacccaa accgtcgccg caccgacccc aaagataact ccccaggtgt
    27601 aatcggtgag ctttgtaaat atctcgacca gactggtgtt aaaaaacatc accaggccgc
    27661 tcaggagcat caatgcccct acaacctgag tgctgcgcat tttctctttc aggataaata
    27721 cgctggcaac catcatgcca actggcgaga gttgcccaat cacctgcgaa gcggtcggac
    27781 tcaggtattg caaggatgag ctgaacagga tgaagttccc aaacagcccg gcggtcgcca
    27841 ctgccaacaa aatcaaccag cgtggcttac gaaacacgcg taatggcggc aacctcttct
    27901 tcaccgcaag aatggcacca aggccaatac tcgccatcaa gaaacggtaa aacacgattg
    27961 tcggaggttc catcacctcc agcacctgct tcattgcgat tggcaacgcc ccccagcaaa
    28021 ttgctgtggt gagcgccaaa agaatgccaa tgcctgcctg ctgcttcata ccgtattccc
    28081 tgcaaagcgc atttgcggat taacgtcgca ccatgcgcat ttttaccggt tatcgaatgt
    28141 aaaaagcccc gcaacgtgtt gcggggcttt catccgttac cgggacgcga aaaacttatt
    28201 cagcttcgat gctgataaat ttacggtttt tcgggccttt aacttcgaat ttcactttac
    28261 cgtctgcttt agcaaacaga gtgtggtcac gaccgcaacc tacgttagcg ccagcgtgga
    28321 atttggtacc acgttgacga acgatgatgc tacccgccag aacggattcg ccaccgaaac
    28381 gcttaacgcc caggcgttta gcttctgaat cgcgaccgtt acgtgtggag ccgccagcct
    28441 ttttatgtgc catttgaaat ctctcctcag gtcttaggcg ctgatgccag taattttcac
    28501 atcagtgaac cactgacgat ggccctgctg cttacgatag tgtttacgac gacgaaactt
    28561 aacgatttta actttctcgc cacgaccgtg agcaacaact tcagctttga ttacgccgcc
    28621 atcaacgaaa ggaacgccga ttttgacttc ttcaccgttt gcgatcatca gcacttcagc
    28681 gaactcaaca gtttcgccag ttgcgatgtc cagcttttcc aggcgaacgg tctgaccttc
    28741 gcttactcgg tgttgtttac caccactttg gaaaaccgcg tacatataaa actccgcttc
    28801 cgcgcacacc ttttcaatga ttcagagtgc gctataaata ttcacaatag ggcgcgaata
    28861 ttacgcaaaa cgcacgcctt tgacaagtgc tacagtcaat acacgaagaa aaaaaacaca
    28921 acttgtacgg taacgtttat ctgtgccatt ttttcagtac aatcactcta tattcctaac
    28981 cataaaccct aagttgcctt tgttcacagt aaggtaatcg gggcgaaaag cccggctttt
    29041 gcgatgaatt tagaaaaaat caatgagtta accgcgcaag atatggcggg tgttaatgcg
    29101 gcaatccttg agcaacttaa ttccgacgtc cagctgatca atcagttagg ctattacatc
    29161 gtcagcggcg gcggtaaacg tattcgtccg atgattgctg tactggctgc acgagctgtt
    29221 ggctatgagg gaaatgcgca tgtcaccatt gctgccctga tcgagtttat ccacacggcg
    29281 actctgctgc acgacgacgt tgtggatgaa tcagatatgc gcaggggtaa agctaccgcc
    29341 aacgctgcat ttggcaatgc cgccagcgtg ctggtaggcg attttattta tacccgcgcg
    29401 ttccagatga tgaccagcct cggttcgctc aaagtgctgg aagtcatgtc agaagccgta
    29461 aacgtcatcg cagaaggtga agttctgcaa ctgatgaacg ttaacgatcc ggacatcact
    29521 gaagaaaact acatgcgcgt tatctatagc aaaaccgcgc gtctgtttga ggctgccgcg
    29581 cagtgttccg ggattctggc tggctgtacg ccggaagagg agaaaggcct gcaggattat
    29641 gggcgctatc tcggcactgc tttccagttg atcgacgatt tactcgatta caatgccgat
    29701 ggcgaacagt taggtaaaaa tgtcggcgac gatctgaacg aaggtaaacc gacgctgccg
    29761 ctgctgcatg cgatgcatca tggcacacca gaacaggcac agatgatccg taccgccatc
    29821 gaacagggta acggtcgcca tcttctggaa ccggttctgg aagcaatgaa cgcttgtgga
    29881 tctcttgaat ggacgcgtca gcgtgccgag gaagaagcag acaaagccat cgcagcatta
    29941 caggtgctcc cggacacccc ttggcgagaa gcactcatcg gcctcgcgca catcgctgtt
    30001 caacgcgatc gttaatcccc tcccttcatc ccgcgcagtg cgcgggatga ttccagtaaa
    30061 ttccataaaa acactaattc agcgctataa cacctgaaaa aaagggcgat gaatctttat
    30121 atattctgaa tattcacact ctttacagga actttttaga gcaataggcc atcaggagta
    30181 tagtgatgcc cgacagaaga agtgttctga atgaaagcga ctaacttaag gagtgaggaa
    30241 agatggaaag taatttcatt gactggcatc ccgctgacat cattgcgggt ttgcgcaaga
    30301 agggaacgtc aatggcggcg gagtctcgca gaaatggttt gagttcctca acgctggcaa
    30361 acgcattatc gcgcccatgg ccgaaaggag agatgattat tgcgaaagcc ctgggaactg
    30421 acccctgggt tatctggcca tcacgctacc atgatccgca gacccatgag tttatcgaca
    30481 gaacgcagtt gatgcgtagc tacactaaac cgaaaaaatg agtggtctgg cggtagcccc
    30541 gcgaacgggg ctgccagctc tcagacgatt actcgccttt cacacgctca atatttgcac
    30601 ctaaagcgcg cagtttgtct tcaatgcgtt cgtagccacg atcgatgtga taaatacgat
    30661 caaccaccgt cgtcccttcc gcaatacagc cagccagcac caggcttgct gatgcacgca
    30721 gatcggttgc cataacctgt gcgccagaaa gtttttcaac accgtgacaa ataacggtat
    30781 tgctttcgat ttcggcatgc gcgcccatac ggctaagctc cggcacatgc ataaagcggt
    30841 tttcaaagac cgtttcggtg atgaatccgg tcccttctgc caccaggttc aacagcgtga
    30901 actgggcctg catatcggtc gggaatgccg gatgcggcgc ggtacgtacg ttaacagcct
    30961 tcggacgttt gccatgcata tccaggctaa tccagtcttc gccgacttcg atgtccgctc
    31021 cagcgtcacg cagtttcgcc agcacggcgt cgagagtatc tggctgcgcg ttacggcaga
    31081 taattttgcc gcgagaaatc gccgccgcca ccaggaaagt accggtttcg atacgatccg
    31141 gtagcacgcg atagacaccg ccgcctaaac gttccacacc ttcgatgacg atacgatcgg
    31201 tgccctgacc gctaattttc gcacccagcg taatcaggaa gttcgcggta tcgacgattt
    31261 ccggttcacg cgctgcgttt tcaataatcg tggtaccttc cgccagggtt gcagcacaca
    31321 tgatggtcac cgttgcgcca acgctgactt tatccatcac gatatgtgcg cctttcagac
    31381 gaccatcgac ggaagcttta acgtaacctt cttccagttt gatggtcgcg cctaattgtt
    31441 cgaggccaga aatgtgtaga tcaaccggac gcgcaccgat cgtacaaccg ccaggcagtg
    31501 aaacttgccc ctgaccaaag cgcgctacca gcggccccag tgcccagata gaagcacgca
    31561 tggttttaac cagatcgtaa ggtgcgcaga atacattaac gtcgcgggca tcaatatgca
    31621 cagaaccatt acgttctact ttcgcaccca gctggcttag cagcttcatt gatgtatcga
    31681 cgtctttcag tttcgggacg ttctggatct ctaccggttc ttccgccagt agagcggcaa
    31741 aaaggatagg cagagcagca tttttagcgc cggaaattgt gacttcgccc tggagcttcg
    31801 ttggcccctg aacacgaaat ttatccattt agtttgttct cagttaacaa ttcatatccg
    31861 ctaccggcga atcgcccata gctcaaaagc cgttcagttt gcgatcgcgc gcccactccg
    31921 caggggtata cgctttgatc gacacagcat gaatgcggtt atccgcaata tattccatca
    31981 gcgggccata gaccgtctgc tgttttttaa cccgactcat gccgtcaaac aactcaccca
    32041 cggcaataac ctgaaagtgg ctgccatcgc cggaaacgtg gacttcctgg agggagagag
    32101 cgttcatcaa cacgctctga atttcattat tttccatggg atcttcaatc atcagttaat
    32161 aaaccagcga aacatcttag agcaaagttg cgctggcata aataagcaaa aagcctcgct
    32221 gataaatcag acaaggctcg acttgcaggc aggtttgccg gacaggcggt taacgccata
    32281 tccggcctga aaaaatttaa cgaggcagaa catcagcagg caaattatac aatttcgcga
    32341 gggtatacac tttgtcgttt accccctgaa gcgtcacgtt gttgccctgc tttttcgcca
    32401 gatcgataag atggagcagc agtgccagtc cacccgtatc cacgcgggac acacggctaa
    32461 gatcgatgca ggtaatcccc ttcaccgctt cctcacgcat ttcccaaagc ggtagcagaa
    32521 cgtcctgatc cagctctccg gataacgcca gcgtgtcacc cgtctgcatc cagctcagtg
    32581 actcgctcat tattttttct cttccagagt gattttctgt tgagaaatcg atttcagttg
    32641 cgcagtcagg ccgtcgatac ctttggtacg cagcagcgtt ccccactcgt tttgtttggt
    32701 ggtgatcata ctgacgcctt cagcaatcat gtcgtaagcc tgccagttgc ccgtctggga
    32761 gtttttacgc cactggaagt ccagacgcac cggcggacgg ccattcgggt caataatggt
    32821 aacgcgaata ggcacaatgg ttttatcgcc cagcggctgt tctggcgcaa tctgataggt
    32881 ttgaccgtga tacatcgcca gcgcctgacc gtaagcctgc ttcaggtact cacggaaagc
    32941 ggcaaagtag gcttcacgtt gagcaggggt cgcactcttg taatactggc ccagcaccag
    33001 cgcaccggcg tatttcacct gtacgtatgg cagcagttcc tgatcaacaa tggtacgcag
    33061 ataatccggg ttggcccgaa tttgcggttg ctcattcttc aggcgatcga acgttttctg
    33121 cgccgcctcg tccatcagct tataaggatt ggtctggtct gccgcggttg ccgcactcag
    33181 aggtgcaatc accagcaaag cgaccatcat taaacgttta aacatgcgtc ggttctcctg
    33241 aaattatttc gttgtaccca caggttcagt ggtttcatta ttacctggcg cagcagctgg
    33301 cgcatcgcca ctattcttat tgtcatcgcc tttactaccg taaaggaact gaccaatgag
    33361 atcttccagc accatcgcag acttagtgtc ctgaattgta tcgccatcct tcaggatagc
    33421 agtccccagt tccgggtctt caaaaccgac gtttaatgcc agatattgtt cccccagcag
    33481 gccggaagta cgaatgctca gcgaactggt atctggaatg tggttataac gttgttcaat
    33541 ttccagcgtt acgcgcggca gataggtttt cgggtccagc gtaatatccg ccacccgacc
    33601 cacaacaacg ccaccaatac tgaccggaga gcgcgctttc aggccgccaa tgttatcgaa
    33661 cgtcgcataa agcgtgtagg tcggttcagt acgtatggac gtcacgttcg ccgccttcag
    33721 gcaaacaaac agcgccgcca gcagtgctgc taataaaaag atacccaccc aaatttcatt
    33781 ttttttcgtt tgcatgaact caattcccaa acatcaatgc ggtcagcaca aaatccagcc
    33841 ccagaacagc cagagacgag tggacaacgg tgcgagtggt tgcccggcta atcccggcag
    33901 acgtcgggat ggcgtcgtag ccgttaaaca acgaaatcca cgtcaccgtg atggcgaaca
    33961 ccacgctctt aatcagacag ttgaccagat ccatacgcca gtcgacggca ttttgcattg
    34021 ccgaccagaa gaacccgcta tcaatgcctt tccaactgac gccgaccaga gatccgcccc
    34081 agatccccac ggcgacgaaa ataaccgtca acagtggtaa tgaaataacc ccagcccaga
    34141 aacggggaga aataacccga cgcagcggat ccaccgccat catctccata ctggagagtt
    34201 gctctgtagc gcgcatcagg ccgatttctg cggttagcgc cgaaccagca cgcccggcaa
    34261 acaacaacgc ggcaaccacc ggccccagtt cacgcagtag cgataacgcc accagcatac
    34321 ccagactggt ttccgcacta taagtggtca gaaccagata accttgcagc cccaacacca
    34381 ttccgatgaa cacgccagaa accacaataa tcagcatcga caggacgccg acattataga
    34441 gctggcgcac cagcagcggc gcatgtttgc gaaattccgg tttgccgacc agcgcattga
    34501 ataacattaa cccggcccgc ccgaacgttc tcagggtttt aatcccttta tgtccgagcg
    34561 acgccagcgc atttaacagc atgagtggct taactccctg gtaaaagatc agcgtgataa
    34621 tcgccggcag gatagcggaa cggaacaggc ccgtcagcta tcccgtccag aaactgacgt
    34681 acgcgcggat caggattcgc ctgcaacgcc tgggcactgc catgagcgac aatttttttg
    34741 tccgccagga tccaggcgtg atccgcaata cttaacactt ccggcacatc gtgagaaacc
    34801 accacacaag tcacgcccag cgcgctgttc agctcagaaa tcagcttcac cagtacgccc
    34861 atggtgatgg gatcttgccc aacaaaaggt tcatcaaaca tgatgagatc cggctccagc
    34921 gcaatcgcac gcgccagcgc tgcacgccgc gccatcccac cagaaagttc agaaggcatt
    34981 agcttagccg ctccacgcag ccccacggcc tccagcttca tcatcactgt actatgcaac
    35041 aatggcgcgg gaagttgggt atgttcgcgc agtggatagg cgacgttgtc aaatacgttc
    35101 atatcagtga acaacgcccc ggactgaaat aacatgctca tccgtttgcg cactgtatac
    35161 aggcgcgaac gagacatcgc cggaatattc tcaccatcga aaaggatctc accatgatct
    35221 ggtgcgattt gcccgccaat cagacggagt agcgtcgttt taccgatgcc cgatggcccc
    35281 atgattgccg tgatcttccc tcgcggcacg gtcagggaaa tattatcgaa gatgcagcga
    35341 ttgccacgcg taaaactgac atcgcgcata tcgactaaat tcgccacaga ctgctccata
    35401 attcaccctt cgtcttgcgt tgattttcta agcatggcgc tcaatttaac cttgaaccca
    35461 acatatttac agaatattac ccgccgtggt tagcgaaagc tggcatttgt tttacttttt
    35521 agccgcataa agtcaaaatt aagaatccgt tacggctttc tgaaaatctt cagcggaccg
    35581 gcgagtatac ctgaagaaag gacgttagat gcttttagct acggcactgt taattgttgg
    35641 tttacttttg gtcgtttaca gtgccgaccg cctggttttt gccgcgtcta ttctttgccg
    35701 aacctttggc atcccgccgc tgatcatcgg catgacggtg gtcagtattg gtacatcgtt
    35761 accagaaatc atcgtctcgc ttgccgcgtc tctgcacgaa caacgcgatt tagccgttgg
    35821 tacagccctc ggctcaaaca ttatcaatat attgctgatc ctcggtctgg ctgcgctggt
    35881 tcgtcctttt accgtccatt ctgatgttct acgccgtgaa ttacccttaa tgttgttggt
    35941 cagcgtggtg gccggttccg tactctatga cggacaactt agtcgcagcg atggtatctt
    36001 tctcctcttt ctggctgtgc tatggctgct gttcattgtt aaacttgcac gtcaggctga
    36061 acgtcagggg actgacagcc tgaccagaga gcagcttgca gagctgccgc gtgacggcgg
    36121 attgcccgtc gcgtttttat ggctcggcat tgcgcttatc atcatgccag tggccacgcg
    36181 gatggtggtt gataacgcca cggtgctggc gaattacttt gccatcagcg agttgacgat
    36241 gggtctgacg gcaattgcta tcggaaccag cctgccggaa ctggcaaccg caatagcggg
    36301 ggttcgcaaa ggtgaaaacg acattgctgt cggaaatatc attggcgcaa acatttttaa
    36361 tattgtcatc gtgttgggtt tacccgcgct gataacgcca ggagagattg atccattggc
    36421 gtacagtcgt gactacagcg tgatgttgct ggtgagcatt atttttgcgt tgctgtgctg
    36481 gcggcgctcc ccgcaaccgg gccgtggtgt aggggtatta ttaactggcg gatttatcgt
    36541 atggctggcg atgttgtact ggttatcgcc aatactcgtt gaataactgg aaacgcatta
    36601 tgtcgcacgt agagttacaa ccgggttttg actttcagca agcaggtaaa gaagtcctgg
    36661 cgattgaacg tgaatgcctg gcggagcttg atcaatacat caatcagaat ttcacgcttg
    36721 cctgtgaaaa gatgttctgg tgtaaaggga aagttgtcgt catggggatg gggaaatcgg
    36781 ggcacatcgg gcgcaaaatg gccgcaacgt ttgccagcac cggtacacct tcatttttcg
    36841 tccatcctgg tgaagccgcg catggtgatt taggcatggt caccccacag gatgtggtga
    36901 ttgctatctc taactcaggt gaatccagcg aaatcacggc cttaattcca gtgcttaagc
    36961 gtcttcacgt accgttaatc tgcatcaccg gtcgcccgga gagcagcatg gcgcgcgccg
    37021 cagatgtgca tctgtgtgtt aaagtagcga aagaagcctg tccgttaggg ctggcaccga
    37081 ccagcagcac caccgccacg ctggttatgg gcgatgccct cgctgtcgcg ctgttaaaag
    37141 cacgcggctt tactgctgaa gattttgcgc tctcacaccc aggcggcgca ctgggtcgta
    37201 aacttctgct gcgcgtaaac gatattatgc atacgggcga tgagatcccg catgttaaga
    37261 aaacggccag tctgcgtgac gcattgctgg aagttacccg caaaaatctt ggtatgactg
    37321 tcatttgcga tgacaatatg atgattgaag gcatctttac cgacggtgat ttacgccgtg
    37381 tcttcgatat gggcgtggat gttcgtcagt taagtattgc cgatgtgatg acgccggggg
    37441 gaatacgtgt gcgccctggc attctggccg ttgaggcact gaacttaatg cagtcccgcc
    37501 atatcacctc cgtgatggtt gccgatggcg accatttact cggtgtgtta catatgcatg
    37561 atttactgcg tgcaggcgta gtgtaaagat tcaaggataa acaacaatga gcaaagcagg
    37621 tgcgtcgctt gcgacctgtt acggccctgt cagcgccgac gttatagcaa aagcagagaa
    37681 cattcgtctg ctgatcctcg atgtcgatgg cgtactgtca gatggcctga tttatatggg
    37741 caataatggc gaagagctga aagcgttcaa tgttcgtgac ggttatggca ttcgttgtgc
    37801 gctcacctct gatattgaag tcgctatcat taccgggcga aaggctaaac tggtagaaga
    37861 tcgttgtgcc acattgggga tcactcactt gtatcagggg cagtcaaaca aactgatcgc
    37921 ctttagcgat ctgctggaaa aactggcgat tgccccggaa aatgtggctt atgtcggcga
    37981 tgatctcatc gactggccgg taatggaaaa agtgggttta agcgtcgccg tggccgatgc
    38041 gcatccactg ttgatcccgc gcgccgatta cgtgacgcgc attgctggcg gtcgtggcgc
    38101 agtgcgcgaa gtttgcgact tattactcct ggcgcagggc aaactggatg aagccaaagg
    38161 gcaatcgata tgagtaaagc cagacgttgg gttatcattg tgctatcact ggcggttctg
    38221 gtgatgatcg gcattaatat ggccgaaaaa gacgataccg cccaggtggt cgtcaacaac
    38281 aatgatccca cctataaaag cgagcatacg gacacgctcg tctataaccc agaaggggca
    38341 ctaagctatc gattgattgc tcaacacgtt gaatattatt ccgatcaggc cgtttcgtgg
    38401 tttacgcagc cggtacttac cacgtttgat aaggataaaa tcccgacatg gtccgtaaaa
    38461 gcagataaag ccaagctgac caatgaccgg atgctctatt tatatggaca cgttgaagtc
    38521 aacgcacttg tgccagactc tcaacttcgc agaatcacga cggataacgc gcagatcaat
    38581 ctggtgacgc aggatgttac ctctgaagac ctcgtcacgt tatacggaac aacatttaac
    38641 tccagcggtc tgaaaatgcg cggcaactta cgcagcaaga acgccgagct gattgaaaag
    38701 gttagaacat cctatgaaat tcaaaacaaa caaactcagc cttaatcttg tgcttgccag
    38761 ctcacttctg gccgccagca ttccggcatt tgccgtaacc ggagacactg atcagccgat
    38821 ccacattgaa tcggaccagc aatctcttga tatgcaaggc aacgtggtta cctttaccgg
    38881 taatgtcatc gtcacccagg gcaccatcaa aattaatgcc gacaaagtgg tcgttacccg
    38941 tccgggcggc gaacaaggta aagaagtgat tgacggctac ggtaaaccgg caacgttcta
    39001 ccagatgcag gacaacggta aacccgttga aggtcacgct tcccagatgc actacgaact
    39061 ggcaaaagat tttgtcgttc tgacgggtaa tgcttatctg cagcaggtcg atagcaacat
    39121 taagggcgat aagatcactt acctggtgaa agagcagaaa atgcaggctt tcagcgacaa
    39181 aggcaagcgc gtaaccaccg ttctggtgcc gtcgcagctg caggataaaa acaacaaagg
    39241 ccagaccccg gcacagaaga agggtaatta attcgttatg gcaacattaa ctgcaaagaa
    39301 ccttgcaaaa gcctataaag gccgtcgcgt ggtagaagac gtcagcctga ccgtcaactc
    39361 cggggaaatt gtcggtctgc tggggccaaa cggtgccggt aagaccacca ctttctatat
    39421 ggttgtaggc attgtgccgc gcgatgcggg caacatcatt attgatgatg acgatatcag
    39481 tctgctgcct ctgcatgcac gcgcgcgccg cggtatcggc tatctgccac aggaagcctc
    39541 cattttccgt cgcctcagcg tttacgataa cctgatggcg gtactgcaaa ttcgtgacga
    39601 cttgtctgct gaacaacgtg aagaccgcgc gaacgagctg atggaagagt ttcacattga
    39661 gcacctgcgt gacagcatgg ggcagtcact ctccgggggt gaacgtcgcc gtgtagaaat
    39721 tgcccgcgca ctggctgcga atccgaaatt tattctgctc gacgaaccgt ttgccggtgt
    39781 tgacccgatc tcggttatcg acattaaacg catcattgag cacctgcgcg acagcggcct
    39841 gggcgtgctg atcactgacc acaacgtgcg tgaaacactg gcggtttgtg aacgcgctta
    39901 tatcgtcagt caggggcatt tgatcgccca cggcacgcct acagaaatct tacaagacga
    39961 acacgttaag cgtgtatacc ttggggaaga cttcagactc tgatagggta gaagtttgcg
    40021 acgttttagc aggagagtac gattctgaac atgaagcaag gtttgcaact caggcttagc
    40081 caacaactgg cgatgacgcc acagctccaa caggcaattc gtctgttgca gttgtcgacg
    40141 ctggaacttc agcaggagct acagcaggcg ctggagagta atccgctgct tgagcaaatc
    40201 gacactcatg aagaaatcga cacccgcgaa acgcaagaca gtgaaacgct ggacaccgcc
    40261 gacgcgctcg aacaaaaaga gatgccggaa gagctgccgc tcgatgccag ttgggacacc
    40321 atttacaccg ctggtacacc atccggcacc agcggtgact acattgacga tgagctgccg
    40381 gtctatcagg gcgaaacgac gcagaccttg caggattacc tgatgtggca ggtcgagctg
    40441 acaccgtttt ccgacactga ccgcgctatt gctacctcta tcgtcgatgc cgttgatgac
    40501 accggttatc tgactgtccc gctggaagat attctcgaaa gtatgggcga tgaagagatc
    40561 gacatcgacg aggttgaagc cgtccttaag cggatccaac ggtttgatcc ggtcggtgta
    40621 gcggcaaaag atctgcgtga ctgcctgcta atccaactct cccaattcga taaaaccacg
    40681 ccgtggctgg aagaggccag actgatcatt agcgatcatc tcgatctgtt agccaatcac
    40741 gacttccgca ctttaatgcg cgtcacgcgt ctgaaagaag atgtgctgaa agaagccgtc
    40801 aatctgatcc agtcgctcga tccgcgcccc gggcagtcga tccagactgg cgaacctgag
    40861 tatgtcattc cagatgtgct ggtgcgtaag cataacggtc actggacggt agaactcaac
    40921 agtgacagca ttccgcgtct gcaaatcaac cagcactacg cctcgatgtg caataacgcg
    40981 cgcaacgatg atgacagcca gtttatccgc agcaatctgc aggatgccaa atggttgatc
    41041 aagagtctgg aaagccgtaa cgatacgcta ctgcgcgtga gtcgctgtat cgttgaacag
    41101 cagcaagcct tctttgagca aggtgaagaa tatatgaaac cgatggtact ggccgatatc
    41161 gcccaggctg tcgaaatgca tgaatcgacg atatctcgcg tgaccacgca aaaatacctg
    41221 catagtccac gaggcatttt tgaactgaag tatttctttt ccagtcacgt caataccgag
    41281 ggcggcggcg aagcttcctc cacggcgatt cgtgcactgg tgaagaaatt aatcgcggcg
    41341 gaaaacccag cgaaaccgtt gagcgacagc aagttaacct ctttactgtc ggaacaaggt
    41401 atcatggtgg cacgccgcac tgttgcgaag taccgagagt ctttatccat tccgccgtca
    41461 aaccagcgta aacagctcgt ttgacccaac cgataaggaa gacactatgc agctcaacat
    41521 taccggaaat aacgtcgaga tcaccgaggc actgcgcgaa tttgttacag ctaaatttgc
    41581 caaacttgag caatattttg accgaatcaa ccaggtctat gttgttctga aagtggagaa
    41641 agtcacccac acctcagatg caacactgca tgtaaacggt ggtgaaattc atgccagcgc
    41701 ggaaggtcag gacatgtacg ctgccattga tggcttaatt gataagctgg cacgccagtt
    41761 gaccaaacat aaagataaac tgaaacaaca ctaattgtcc gggcaattag catgtgcatg
    41821 gcggcctgtt gtgcggcaca acgggccatt tgtacggtta atgctccgag cctgttccac
    41881 tgtttgagtg ggcaggttct taggtgaaat tatgacaaat aatgatacaa ctctacagct
    41941 tagcagtgtt cttaacaggg aatgtacgcg aagccgcgtc cactgtcaga gcaaaaaacg
    42001 cgccctggaa atcatcagcg agctggcggc gaaacaactt agcctgccgc ctcaggtggt
    42061 ttttgaagct atcctgacgc gggaaaaaat gggcagtacc ggtatcggca atggtattgc
    42121 cattccgcat ggcaaactgg aagaagatac tctgcgcgcc gttggcgttt tcgttcagct
    42181 cgaaacgcct atagctttcg atgccatcga caaccaaccg gtggacttac tttttgccct
    42241 gctggtgccg gcagaccaaa ctaaaacgca cctgcatact ctgtcgctgg tggcgaaacg
    42301 tctggcggac aaaaccatct gccgccgttt acgcgcagcc cagagcgatg aagagctgta
    42361 tcaaatcatt acggataccg aaggtactcc ggatgaagcg tagttattcg gtaatgtctc
    42421 ttttagacgt tgtgaggaga aacagtacat ggtactgatg atcgtcagcg gacgttcagg
    42481 ttcaggtaaa tctgtcgccc tgcgtgcgct ggaagatatg ggtttttact gcgtggataa
    42541 ccttcccgta gtgttgttac ccgatctggc tcgaactctg gccgatcgcg agatttctgc
    42601 cgccgtcagc attgatgttc gtaatatgcc ggagtcacca gaaatattcg aacaggcgat
    42661 gagtaacctg cctgacgctt tctcaccgca actactgttc ctggatgccg accgtaatac
    42721 cttaattcgt cgttacagtg acacgcgccg actgcatccg ctttccagca aaaacctgtc
    42781 gctggaaagt gctatcgaca aagaaagcga tttgctggag cctctgcgtt cgcgagcgga
    42841 tctgattgtc gatacctcag aaatgtccgt tcacgagctg gctgaaatgc tgcgtacccg
    42901 tctgctgggt aaacgtgaac gcgaactgac catggtcttt gagtctttcg gcttcaaaca
    42961 cggtatccct atcgatgcag attacgtctt tgacgtgcgc ttcttgccga acccgcactg
    43021 ggatccgaaa ctgcgtccaa tgacaggtct tgataaacct gtcgccgcgt tcctcgaccg
    43081 ccacacagaa gtacacaatt ttatctacca gacgcgaagc tatcttgaac tatggttacc
    43141 tatgctggaa accaacaacc gtagctactt gacggtcgcc attggttgta ccggcgggaa
    43201 gcaccgttcg gtgtatattg cagagcaact ggcagactac ttccgctcgc gcggtaaaaa
    43261 cgtccagtca cgccatcgta cgctggaaaa acgtaaacca tgaccgtcaa gcaaactgtt
    43321 gaaatcacaa acaagctggg catgcatgcc cggcctgcaa tgaagctgtt tgaattaatg
    43381 cagggttttg acgctgaagt gctgttacgt aatgacgaag gcaccgaggc cgaagccaac
    43441 agcgttattg cgctgctgat gttggattct gccaaaggac ggcagattga agttgaagcg
    43501 accggcccac aggaagagga agcactggcc gccgttatcg ccctctttaa ctctggtttt
    43561 gatgaagatt aatcttcatc actttgacat acaactccct tcaaactccc cccgacaata
    43621 agaaaatcac gtactgaaat cgttctcaat caacgtcatt tgtacatttt gtgcgctttt
    43681 cactttcaga agaaccttaa gaaaacctta agaggcattg tttaggtttt gtttaagtta
    43741 atcgaccata ctggagatcg tcagaaaata tttccaggag atggcatgat tcgcttatct
    43801 gaacaaagtc ccctgggcac cggaagacat cggaaatgtt atgcgcatcc ggaagatgcc
    43861 caacgctgta tcaagattgt ctaccatcgt ggcgatggtg gcgataaaga gatccgccgt
    43921 gagttaaagt actacgcgca tcttggtcgc cggttaaaag actggagtgg aataccgcgc
    43981 tatcacggta ccgttgagac ggattgcgga accggttatg tctacgatgt gattgcagat
    44041 tttgacggta aaccctccat tacgctgacc gaatttgctg aacaatgccg ttacgaagaa
    44101 gacattgcac aactgcgcca gctactgaaa cagctgaagc gttatttgca ggataaccgt
    44161 atcgtgacga tgtcgctgaa gccgcagaat atcctctgtc accgcatcag tgagtcagag
    44221 gttatcccgg tcgtctgtga caacattggt gaaagcacat tgattccact ggcgacctgg
    44281 tcgaagtggt gttgtctgcg taagcaagag cggttatgga aacgatttat cgcccaaccg
    44341 gcgctggcta ttgccctgca aaaagatctg caacctcgtg agagcaaaac gctggccctc
    44401 acttcgcgcg aagcttaatc cagctggtgt tgttgcataa acggctcacc gcctaactga
    44461 tacatctgcc gtaaaatcca cgcctgacgg ctacgcacgt agcccgatgg cgaggagact
    44521 ttgaaacgaa gtggattagg taatacagct gccagtaatg cagcttccga ccgggtaagt
    44581 ttgctcgcgg gtttgtggaa ataacgttgt gccgcagctt cgacgccaaa cacaccgtcg
    44641 ccaaattcgg cgatattcag gtaaaccgtc aggatacgct ttttgctcca gactgtttct
    44701 atccccagcg ttaatccagc ttccagccct tttcgtaccc agctacgccc atcccataaa
    44761 aagagatttt tggccgtctg ctgagaaatc gttgaagcac cgcgaatacg gttttcattg
    44821 cgctcgttgt gcgccagggc tttctcaatg gaagcgacat caaagcccca gtgctcagga
    44881 aatttctgat cttctgcggc aatcaccgcc agtcccatcc acggcgagat ttgatccata
    44941 ctgacccagt cagaatgtgc cacgtaacga aaattgccat gcagccaggc gctgacctgt
    45001 cgctcgacca ttaccgctga gaagggaaca ggcgcaacac taaacaacgc gatgcccccg
    45061 ccccagaaaa cggcgaggac aaccattaac cgcaaaagaa aacggcgaac gaaactaaac
    45121 accgttaagc ggcttttact cattcagcca gaaccagcac gcgggaaacc agcttatcaa
    45181 tgccgctcgc cgcttctgca atgttctgcg ccagcatata tgctggggtg gtgacaatct
    45241 tattgtcttc atcaaccacg atatcatcga caggacaggg cacatgctcc gcgcccatct
    45301 cttccagcac ttctgcggta tcgatatcag taccgatggt caaacgcagc gggaaatcga
    45361 aaattttcgg cagcatcgcc ggggcaatac acataaaacc aagcggtttt ccggcctgat
    45421 gcatcgcttg tgccagcgcc tttaattcac ggtcaacggt gcattcgcta ccaagagtgg
    45481 caaaattgct taaattcttc gccgcgccaa acccccccgg cacaatcaac gcatccagtt
    45541 cagcggcatc ggcctgggcc agaggacgga tttcaccgcg cgttattcgt gccgcttcaa
    45601 tcagcacgtt gcgcgtttcc gtcatcgctt cgccagttaa atggttgata acatcaacct
    45661 gctgcttatc cggtgcaaag cagaccgcct gcgcaccgct gcgtgaaata gctaacaacg
    45721 tcaacaccgc ttcatgaatt tcagaaccgt catagacgcc gcatccgctc agaattacgc
    45781 caattttctt cattgtgatc atcctttcgc aattgactga aacacattaa tttttttaat
    45841 aaaaatggta cgcatcacac atttaactga ttcatgtaac aaatcattta agttttgcta
    45901 tcttaactgc gtgcggcctg aaaaacagtg ctgtgccctt gtaactcatc ataataattt
    45961 acggcgcagc caagatttcc ctggtgttgg cgcagtattc gcgcaccccg gtctagccgg
    46021 ggtcattttt tagtggcttt tgccacccac gctttcagca cttctacgtc gtgacgccac
    46081 tcttctttca tctcttcaat ccattcaccg acgttatctt cccaggcagg aaggtccgga
    46141 gactgaattt gctgacccag ctgttgcaga tggcgtaacc ccactgaccc cgccgcacct
    46201 ttaattttat gtccttcctc tacaatgcct tttttatcct gcgccgtcag attcgactcc
    46261 agcacgctga catagcccgg catcatcttc tcaaacaccg ctaacccgtc ggtgatcagc
    46321 ttcggtccta caagttcgag atactgttcc agcatgggaa tatcgagcaa tgcttctgat
    46381 ttactgttct cttctgtcgt caccgtactc tcctcatcat cctgggtatc ccagaatttc
    46441 ttgatcatcg cggttagcgc cggaaccgaa agcggcttac tcagcacatc atccattcca
    46501 gcattgaggt actcttgttt gtctttcagc acgttagcgg ttaaggccac cagcggcggt
    46561 aaatcctcgc gcggataacg cttcgtcagt tcacgagaga tatccagccc ggtcatatct
    46621 ggcaactgaa tatccagcaa caccaggtcg tattcgcccg gtttaaacat ctccagcgtc
    46681 gccttgccgg tcatggcgac atcaacgctg ttacctaatt tttccagcac agaacgcgca
    46741 acaatcacgt tcagttcaat gtcttccacc agcagcacat tcagcgccgg taaaggcata
    46801 tcgtcttcat caaacgcatc atcgacctct tctgctaccg acggtgcgtg gatcgtcaac
    46861 gtaaaggttg aacctttgcc ctgttcgctg gtaaccgtaa tatcgccgcc catatttttc
    46921 gccagacgac gagaaacggc cagaccaata ccggtgccgg tggcaggttt accgccatga
    46981 ctgtctttca cctggtaata catggcgaaa attttatcca gctcatcctg cggaatgccg
    47041 ataccagagt cttccacttc aaaatgcagc atatcgcctt catcgtagcg cacgcgcacg
    47101 gtaacctggc cttgctgggt gaatttgacg gcgttactga tgaggttcca caggatctgc
    47161 cgtaaacgcg tcccgtcggt aatgacctga tgcggtaatg gcagcgtcgg ctccaggtta
    47221 aagcgcaatc ctttttgttg cgcctgcaat gcggagagat tttccagatc ggcaaggaag
    47281 ctggtgaaat caaccggttg attatcaagc tggaccttgc gccgttccat cttatccatg
    47341 tcgataatat cgttaaagat attccccagc gtgacggccg aaacatggat ggtcttgaga
    47401 tatttttcct gctcggcggt gagttcggta tccagcagaa tacggctcag gccgacgata
    47461 ccgttcagcg gtgtacgcaa ttcgtgactg atggtggaaa taaacgtcgt tttgtcgcgg
    47521 ctggcccgtt caagcgcatc ctgataccgc ttacgctcgg taatgtcgcg accaaagccc
    47581 atcaaaccgt gacgtttacc cacgcggtcg tagtacggca ctttacggat ttcaaagcag
    47641 gctttgcgcc cgtccgggta atccagccac tgttcatagg tcagtgacac attatgacgg
    47701 aacacttttt catcggtttc aatgactttt gcggcggctt ccggtgagta aacatcagca
    47761 ggtttcaggt gaaccagttg tttttcgctt tttccggtca gcagctccat cgcgcggtta
    47821 cagccggaaa actctttatc ttcgttacga taaaaaacca ggtcgggtga agcatcaagg
    47881 aaggaacgta agaatgagga ttgctgctcg agctgaattt gtgtctcttc gcgctctttg
    47941 atttcaattt tcagttggcc gaaggtttcc tgcagttctg cttccgcttt ttcacgaacg
    48001 gcaatttcct gatttagctg ggcaatatta tcttttaact gaacgttgag gctcaaatcg
    48061 cgctcgcgca tctcctccag tttttgcacc agccgtgaca gacgttgtcg tgactcctcc
    48121 agttgctcga cgaccaccga tagaaagtag accgcccacg gcgtaatcag caaaccaaag
    48181 aagatagaac gaataacatc aatgctttcg acctgaccat gcagcaccat ggttaccgcc
    48241 atttgtacca caatggcaag aacgacgagg gccagcgcca gcaacattga gaagcgcacc
    48301 agacctaact tcatcatcag gtcaacataa tactgcgcca gcagacgaat ttgcttcatt
    48361 agggaattcc ttcacgacaa cctgccccaa taatacccaa ttctgacagt tacgttataa
    48421 attgtgtgag aaatgcggaa ttcccctcaa cgacctactc cgttggaggt atccacggac
    48481 gttccagcgc cgatccctgt acgccatgtt cattcagata gcgatccagc tcgaccattc
    48541 ccgtccatcg attttcgcac cacagaggag caagcagcgt cgggcgacgg gcgctggcgg
    48601 aaatgcggtg gtagatcact tccggtggcg tatggcgaat catttctccg gcagtgagcg
    48661 tgtaatcctc cagttcaata ccgttcaaac gccccgcttc ccaggctttc gccataatgc
    48721 tgcctttcac aatatgcagc ggatgcagct ttatgccgtc cacgccggtt tcaaccactc
    48781 gctccagcgt ttgcaaacat tcagcctgcc cttcgccagg caggccgaca attaagtggg
    48841 aacacacctt cagcccgcgc tgacgtgcca gctgggttgt acgctgataa caggcaaaat
    48901 catggccgcg gttgatgcga tgcagtgttt tgtcgtgggc ggtttgtagc cccagctcca
    48961 gccacacttc gtaaccctgg tccttatatt cgcaaagcag atccagcacc gcatccggca
    49021 cgcagtccgg gcgggtacca acacacaaac cgacaatatt ggcctggctc accgcctgct
    49081 gatacataga acgcagcacc tgaacttccg caaaggtgct ggtatacgcc tgaaagtagg
    49141 ccagatagcg tttagcgcgg ttaactaaat tcgcctggtg cgccagttgc tcggcaatgg
    49201 aacgatgctg ctgcgcttca tcggcaaacg aggcaacatt acagaatgtg cagccaccac
    49261 gcccgatggt accgtcacgg ttagggcagc taaaaccgcc gtgtagcgtc agcttatgca
    49321 ccttttgccc ataacgacgg gtgagatcac caccaaacat attgactaat ttctgtaact
    49381 gcataatctg atagaccgcg ccttgaaaag aggccaaagc ctgccatttt tagctcaatt
    49441 cggcgatgac ctggatcaat cgtcccggcc tgcttttatc aactgcataa tcaatcaaaa
    49501 ttaccgaaat ttcatgcata atcacataaa tcacttttgc ttatcttgtg tcagattttt
    49561 ttatctcctt atggatttta ggcaaaaaca gtagcatgaa acgtcattac caattaaggc
    49621 agtataaaat gctggttttc tcgtcagttc aaggcaggat aagggttaac acgcctttat
    49681 gacagtcagg aattgactgt ttctctaacg acttcccttt tagccttaaa gataaaatcc
    49741 attttaattt cagtaattta ataaagaatt ttgcgctaaa gcacatttct gtaccaataa
    49801 gcttgccatt tgacctgtat cagctttccc gataagttgg aaatccgctg gaagctttct
    49861 ggatgagcag cctgctcatc atatttatgc agtaattgag atcccctctt caccgtatta
    49921 accgatgcga aaaggacaac aagggggcga atgcgaggcg cgcgtatgac acgcaaaccc
    49981 cgtcgccacg ctctttctgt gcccgtgcgc agcggttcgg aagtggggtt cccgcagagc
    50041 ctgggggagg ttcacgatat gttgtacgat aaatcccttg agagggataa ctgtggtttc
    50101 ggcctgatcg cccacataga aggcgaacct agccacaagg tagtgcgtac tgcaatacac
    50161 gcactggccc gcatgcagca ccgtggcgcg attctcgccg atggtaaaac cggcgacggt
    50221 tgcggcttgc tgttacaaaa accggatcgc ttttttcgca tcgttgcgca ggagcgcggc
    50281 tggcgtttag caaaaaacta cgccgtcggg atgctattcc tgaataaaga tcctgaactc
    50341 gccgctgccg cacgccgcat cgttgaagaa gaactgcaac gcgaaacctt gtcgattgtg
    50401 ggctggcgtg atgtccccac taacgaaggc gtgctgggtg aaatcgccct ctcctctctg
    50461 ccacgcattg agcaaatttt tgtgaacgcc ccggcaggct ggcgtccgcg cgatatggag
    50521 cgccgtctgt ttatcgcccg ccgccgcatt gaaaagcgtc tcgaagccga caaagacttc
    50581 tacgtctgta gcctgtcgaa tctggtgaac atctataaag gtctgtgtat gccggcggat
    50641 ctgccgcgct tttatctgga tcttgcggac ctgcgtctgg aatcggccat ctgcctgttc
    50701 caccagcgct tctccactaa caccgtaccg cgctggccgc tggcgcagcc gttccgctac
    50761 ctggcgcata acggtgaaat caacaccatc accggtaacc gccaatgggc gcgtgcgcgt
    50821 acctataaat tccagacgcc gcttatccct gatctgcacg acgccgcacc gttcgtcaat
    50881 gaaaccggct ctgactccag ttcgatggat aacatgctgg aactgctgct ggcaggcgga
    50941 atggatatca tccgcgccat gcgtctgtta gtgccacccg cctggcagaa caacccggat
    51001 atggacccgg aactgcgtgc cttctttgac tttaactcca tgcatatgga gccgtgggat
    51061 ggcccggcgg gcatcgtgat gtccgacggt cgttttgccg cctgtaacct cgaccgtaac
    51121 ggtctgcgtc cggcgcgcta cgtcatcacc aaagataagc tcatcacctg cgcctctgaa
    51181 gtcggtatct gggattacca gcctgacgaa gtggtcgaaa aaggccgcgt cgggccaggc
    51241 gaactgatgg ttatcgacac ccgcagtggg cgtattctgc actcggcaga aaccgatgac
    51301 gatctgaaaa gccgccatcc atataaagag tggatggaga aaaacgtccg ccgactggta
    51361 ccgtttgaag atctgcccga tgaagaagtg ggtagccgcg aactggacga cgacacgctc
    51421 gccagctatc agaaacagtt taactacagc gcggaagagc tggactccgt aattcgcgta
    51481 ctgggcgaaa acggtcagga agcggtcggt tcgatgggcg acgacacccc attcgccgtg
    51541 ctctccagtc agccgcgcat tatttacgac tacttccgcc agcagtttgc ccaggtgact
    51601 aacccgccaa tcgacccgct gcgtgaagcg catgttatgt cgctcgccac cagtatcggt
    51661 cgtgaaatga acgtcttttg cgaagcagag ggccaggcgc accgtttaag ctttaaatcg
    51721 ccgattctgc tctactccga tttcaaacag ctcacgacga tgaaagagga gcactaccgc
    51781 gcagatacgc tggatatcac ctttgacgtc actaaaacca cgctcgaagc gacagtcaaa
    51841 gagctgtgcg acaaagccga aaaaatggta cgtagcggca ccgtgctgct ggtgctctcc
    51901 gaccggaata tcgctaaaga tcgcctgccg gttccagccc cgatggcggt tggcgcgatc
    51961 cagacccgtc tggtcgatca aagcctgcgt tgcgatgcca acatcatcgt cgaaaccgcc
    52021 agcgcccgcg atccgcacca cttcgccgtg ttgctgggct tcggcgcgac ggctatttat
    52081 ccataccttg cctatgaaac gctgggccgc ctagtagaca cccatgcgat tgccaaagat
    52141 tatcgtaccg tgatgctcaa ctaccgtaac ggcatcaaca aaggcttgta caaaatcatg
    52201 tccaaaatgg gcatctccac catcgcctct taccgctgct cgaaactgtt tgaagcggtc
    52261 ggtctgcacg atgatgtagt gggcctgtgc ttccaggggg cggtcagccg cattggtggg
    52321 gcgagctttg aagacttcca gcaggatctg ctgaatctgt cgaaacgtgc ctggctggcg
    52381 cgcaagccca tcagccaggg cggcctgctg aaatacgtcc acggcggcga ataccacgcc
    52441 tacaacccgg acgtggtgcg cacgctgcaa caggcggtac agagcggcga gtatagcgac
    52501 tatcaggaat acgcgaagct ggttaatgag cgtccggcaa ccacgctgcg cgatctgctg
    52561 gcaattacgc cgggtgaaaa cgcggtcaac attgctgatg ttgaaccggc aagcgaactg
    52621 tttaaacgct ttgataccgc cgcgatgtct atcggcgcgt taagcccgga agcccacgag
    52681 gcgctggcgg aagcgatgaa tagcatcggc ggtaactcga actccggtga aggcggcgaa
    52741 gacccggcgc gctatggcac taacaaagtg tcgcgcatca agcaggtggc ttctggtcgc
    52801 ttcggcgtta ctccggcgta tctggtcaat gccgacgtca ttcagattaa agtcgcccag
    52861 ggcgcgaagc caggcgaagg cggtcagttg ccgggtgata aagtcactcc ttacatcgcc
    52921 aaactgcgct attcggtgcc cggagtgacg ctgatctccc cgccgccgca ccacgatatc
    52981 tactctatcg aggacttagc gcagctcatt ttcgacctca agcaggttaa cccgaaagcg
    53041 atgatctccg tgaagctggt ttccgaaccg ggcgttggca ccatcgccac cggtgtggcg
    53101 aaagcttatg ccgatttaat caccatcgca ggctatgacg gtggcaccgg cgcaagcccg
    53161 ctgtcgtcag tgaaatacgc aggctgtccg tgggagatgg ggcttgttga aacccagcag
    53221 gcgctggttg ctaacggctt gcgtcacaag atccgtttgc aggtcgatgg cggcctgaaa
    53281 acgggtgtcg atatcatcaa ggcggcgatt ctcggcgcag aaagcttcgg cttcggcact
    53341 ggcccgatgg tggcgctcgg ctgtaaatat ctacgtattt gccatctgaa caactgcgca
    53401 acgggtgtag caactcagga tgacaaactg cgtaagaacc actatcacgg cctgccgttc
    53461 aaggttacca attactttga atttattgcc cgtgaaaccc gcgagctgat ggcacagctg
    53521 ggcgtaacgc gtctggtgga tctgattggt cgcaccgacc tgctgaaaga actggacggt
    53581 ttcaccgcca aacagcaaaa actggcgctg tcgaagctgc tggagaccgc cgaaccgcat
    53641 ccaggtaagg cactctactg caccgaaaac aacccgccgt ttgataacgg cctgctgaac
    53701 gcgcagttgc tgcaacaggc gaaaccgttt gtcgatgagc gccagagcaa aaccttctgg
    53761 ttcgatattc gcaacaccga ccgttctgtc ggcgcgtcgc tttcaggcta tatcgcccag
    53821 acgcacggcg atcaggggct ggcagccgat cctatcaaag cgtacttcaa cggcaccgca
    53881 ggccagagct tcggcgtgtg gaacgcgggc ggcgtggaac tgtacctgac cggtgatgcc
    53941 aacgactatg tcggtaaagg catggcgggc ggcttaatcg ccattcgtcc tccggttggt
    54001 tccgccttcc gcagccatga agcaagcatt atcggcaaca cctgcctgta tggcgcgacc
    54061 ggtggtcgtc tgtatgccgc aggccgcgcg ggtgaacgtt tcggcgtgcg gaactccggt
    54121 gctatcaccg tggtagaagg cattggcgac aacggctgtg aatatatgac gggcggtata
    54181 gtctgtattc tgggtaaaac cggcgtgaac ttcggcgcgg gcatgactgg cggcttcgct
    54241 tacgttctcg atgaaagcgg cgatttccgc aaacgcgtta acccggaact ggtcgaggtc
    54301 ttaagcgttg acgctctggc gatccatgaa gagcatctgc gtggtcttat caccgagcat
    54361 gtgcagcata ccggctctca gcgcggtgaa gagattctgg cgaactggtc aaccttcgcc
    54421 actaaatttg cgctggttaa accgaagtcc agtgatgtaa aagcactgct gggtcaccgt
    54481 agtcgtagcg cagctgagtt gcgcgtgcag gcgcagtaag gggtagcaac aatgagtcag
    54541 aatgtttatc aatttatcga cctgcagcgc gttgatccgc caaagaaacc gctgaagatc
    54601 cgcaaaattg agtttgttga aatttacgag ccgttttccg aaggccaggc caaagcgcag
    54661 gctgaccgct gcctgtcgtg cggcaaccca tactgcgagt ggaaatgccc ggtacacaac
    54721 tacatcccga actggctgaa gctcgccaat gaggggcgta tttttgaagc ggcagaactg
    54781 tcgcaccaga ctaacaccct gccggaagtt tgcggacgtg tctgcccgca agaccgtctg
    54841 tgcgaaggtt cctgcaccct gaacgatgag ttcggcgcag tgaccatcgg caacattgag
    54901 cgttatatca atgataaagc gttcgaaatg ggctggcgtc cggatatgtc cggcgtgaaa
    54961 cagaccggta aaaaagtggc gattatcggc gcaggcccgg caggtctggc gtgtgcggat
    55021 gtcctgacgc gcaacggcgt aaaagcggtc gtcttcgacc gtcacccgga aattggcggc
    55081 ttgctgacct tcggtattcc ggctttcaag ctggaaaaag aggtaatgac gcgccgccgt
    55141 gaaatcttca ccggcatggg tattgaattc aaactcaata ccgaagtggg ccgcgacgta
    55201 cagctggacg atctgctgag tgattacgat gccgtgttcc ttggcgtcgg gacttatcag
    55261 tcaatgcgcg gcgggctgga aaacgaagac gccgatggcg tgtacgcagc gctgccgttc
    55321 ctcatcgcca acaccaaaca gttaatgggc tttggtgaaa ccaacgacga accgttcgtc
    55381 agcatggaag gtaaacgcgt ggtagtactt ggcggtggcg acactgcgat ggactgcgtg
    55441 cgcacgtctg tacgccaggg agcgaagcac gtaacctgtg cctatcgtcg tgatgaagag
    55501 aacatgccgg gttcccgccg cgaagtgaaa aacgcgcggg aagaaggtgt tgagttcaaa
    55561 ttcaacgtcc agccactggg gattgaagtg aacggtaacg gcaaagtcag cggcgtaaaa
    55621 atggtacgta ccgaaatggg cgaaccggat gccaaaggcc gtcgccgcgc ggagatcgtt
    55681 gcaggttccg aacatatcgt tccggcagat gcggtgatca tggcgtttgg tttccgtcca
    55741 cacaacatgg aatggctggc aaaacacagc gtcgagctgg attcacaagg ccgcatcatc
    55801 gccccggaag gcagcgacaa cgccttccag accagcaacc cgaaaatctt tgctggcggc
    55861 gatatcgtcc gtggttccga tctggtggtg accgctattg ccgaaggtcg taaggcggca
    55921 gacggtatta tgaactggct ggaagtttaa gctctgtccc agcgccgcct cctcagaaaa
    55981 gagtcggcgc tgaaattctc ctttctttct ggaaagcgcc tcgcataatc tgaagaaaac
    56041 caatgtaata cgctcatctg gattgttacc ggtagatgcc cggcgtatag ctgattccat
    56101 tattcaacgc agcgccatat aaagggacag ataatggaat cgctctcaga gagaacctca
    56161 acaggctacc agcaaatcca cgacggtatt attcatctgg tcgatagcgc ccggacggaa
    56221 acggtacgta gcgttaacgc gctgatgacc gcgacatact gggaaattgg ccgacgaatt
    56281 gtcgaatttg aacaaggtgg cgaggccagg gctgcgtatg gtgcgcagct aatcaagcga
    56341 ctatcaaagg atttaagtct aaggtataag cgtgggttct ctgcaaaaaa cttacgccaa
    56401 atgaggcttt tttacctctt ttttcaacat gttgaaattc gccagacagt gtctggcgaa
    56461 ttaacaccat tgggaattcc gcagacacca tctgcagaat tcccatcggc aaagatttgg
    56521 cagacgctgt ctgccaaatc tttccccctg ccctggtcca cctacgtccg tttactttcc
    56581 gttaaaaacg ctgacgcccg cagcttttat gaaaaagaga cgctccgctg tggctggtct
    56641 gttcgccagc tagaacggca aattgcgacc caattttatg agcgaacact actgtcacat
    56701 gacaaatcag ccatgctgca acaacacgct cctaccgaga cgcatattct tccgcaacag
    56761 gcgatacgcg atccctttgt gctcgaattt ctggaattga aagatgaata ttcagaatct
    56821 gatttcgagg aggcgctgat caaccacctg atggatttca tgctggaact tggggatgat
    56881 tttgcctttg ttggtcggca gcgaaggtta cgcattgatg acaactggtt tcgtgtcgat
    56941 ctgctgtttt tccaccgccg tttacgctgc ctactaatcg tcgatctaaa agtgggcaaa
    57001 ttcagctata gcgatgccgg acagatgaat atgtatctca actacgccaa agagcactgg
    57061 acgctaccgg atgaaaatcc gcccatcggt ctggttctct gtgcagagaa aggagccgga
    57121 gaagcacatt atgctctggc aggtttgcct aacaccgttc tggcaagcga atataagatg
    57181 caactacctg atgagaaacg actcgcagat gaactcgttc gaacacaggc ggtgctagag
    57241 gaaggctata gacgccgtta atttcagcgg tttcgaattc tgcagacagt gtctgcagaa
    57301 tctttaaaac gaactaagtt ttaagccatt aaatcagcct taaccttcac cacaaccttt
    57361 ttaatctctc caggctcgcc gacaacgcat cctggtttat gcggttcacc tggcataaac
    57421 acggcgaaca ttcccggttt taagatgatg gcttgctcgt tatcaatggt gctgcaaagc
    57481 tgataatcat cctcatggtg gaactcttca cactggcgcg cagtgcctgc catgccaaac
    57541 agaatccgtt cctcaccgtt taataacagt tggatatcaa tgtattgctc gtgcaattcc
    57601 gcttttttct cgacgggcga ttgagtgtta aacgtcatga cattcataaa gatattgtcg
    57661 ccctgtaatt cgtaacgacc cggcgctttt tcttgcggtc tggcagctaa tgccagcgtt
    57721 aacgcgtcct gtaacgcagg atgtaaccca gcagacggta atgactgtac ttcacccatc
    57781 atcataattt ttctccctgg gccaacagcg cagccccaag taaacctgca tcgtggcggt
    57841 agtgcgccgc cagtaaatca acatgaaatg ccgctggctc ctgcgccaga tacgtttcca
    57901 ccagcgccag atacccttct gccagcccaa cgctgccacc gaccaccacg cactggcaat
    57961 cagttgtggc tttaatatca gcgatcagcc ttgcaagcgt acgtgcggag cggtgaatca
    58021 gctgctgcgc ctgctcgtcg ccctgcccgg cgcgtgtgaa aatagttctc gcatccgcgc
    58081 ccgccagttc accctgcgct gccgccgcaa tgccgcgacc agaagcaatc gcctccacgc
    58141 aacctgtgcg tccacagcca cagaccgggc cgtgtggatc ggcaagcgta tgcccgatgt
    58201 gccccgccag accgccaggg ccggtaagca gtttgccgcc gctcactaca ccgccgccga
    58261 cgccagtgga aacggtgata aagaccatat cggttatatc gccttccagc gcctgatact
    58321 ctgcccatgc tgcggcctgc gcgtcgttaa tggcaatggt cggcaaattg gtaagttgtt
    58381 ccagcgtttt gactaacgga aagtgtagca atccaccgag attatgcgga ttcagcgcca
    58441 gcaaactgcc gtcgcgaata attccggttg aagcgatggc aacccgctgc gcatgagctt
    58501 gcaacggaga gactaatgcg gataaggcat cacgcaaggc ttctggtgtc tggctggctg
    58561 gcgtaggaag ttcacgacga tcgcggatct gcccgtcagc gccaatcagc gcggcggcaa
    58621 gtttagtacc gccgatatca atcgccagcg tggtcatagc accgcctttt tcatcgctgt
    58681 gttgtaccac tgacaaatgt gttcaagacg cgtaatggca gaaccgaccg tcaccgccca
    58741 cgcgccgtgg cgcatcgcat ctgccgcctg agcaggcgtg ttgtaacgcc cttcggcaat
    58801 caccctacat ccggcgtcgc tcaacgtttt caccagcgcc agatccggct cttctggcgt
    58861 ttcaggcgtg gtatagccag aaagcgtagt gccaataatt tcggctccca gcttttggca
    58921 tgccaggccg tcttccggcg ttgagcagtc ggtcatcgcc agtaaaccgt gatggtgaat
    58981 acgtgccagc agcgtttcaa caggcaccgg acgcgggcgg tcggtgccgt caatggcgat
    59041 aatgtccgcg cccgcctgcg ccagcgcatc aacatcttca atataggccg tgatgcgtac
    59101 cggagaatcc tccagatcgc gtttcacaat tccaataatc ggcacgctca ccaccgcacg
    59161 cgtggcttgc agatttgcca caccttcaat gcgaatggca accgcgcccg cctgttctgc
    59221 cgctaatgcc atggcggcga cgatttcggg tttatcgagc gggctgtccg gaaccggctg
    59281 gcaggagaca atcaggccac cgttagcagc gattttttga tccagttgtg caagtaacga
    59341 catacatctt cccttagcga aaggcccggt acatagaccg ggcaacagga ttaacttttg
    59401 gttttgacta aatcgttttt ggcgctgcca aatggcacgg caccgctaaa tggtttgccg
    59461 tcgatagcgt catgagtacg caatgcttcc gggcgcagcc aacgctgaac gcgggaaggc
    59521 atatccagac caatcagcag gatcaccaca aatgtcagac tgaacgatag cgatgccagc
    59581 gcagtaccca gatccagacg ttgagcgatc aacgcgccga tgattggggc cagtgcgccg
    59641 cccaatgcgc caacgttgta ggtaaagccc aggcccgctg cacgctggtc ggtatcgaaa
    59701 taaccgccaa tcagtttcgg taagatcccg gcgatccctt gtccaagcat ttgctggaag
    59761 aacagtaaca gaccgagcac ccagacgttt gcgccgccaa tcgcaaatac cggaataatc
    59821 aacagctgcg aggccaacag gctacaaacg taggctttgc gggttcccag ccagtcaccg
    59881 aggaagccgc cgacacagca ccccaccgca gccccaaagc cgctaaagaa cagcacattg
    59941 gcgacagtat gcgggtcata agccagatcg gttttcagat acgttggcag cagcgcctga
    60001 atcggccatg agtagaggaa agcaaacaac acgaccacca tcagcattac gcccgttggc
    60061 cagcgtttgc ccgtactctg caccataaag ctgataaaga ttgcggcgca taacagccca
    60121 agaacagcga cgatcgcggc attttgcagg ttaccggcga agcagaacca cagcgcagta
    60181 gccgccgcca gtgtcattac gatattggca atgcgatgtt caccacggta gagaatatcc
    60241 accattgtgc gtaccggtgc tttacccccg tgtttctctt tccagtcttc cgcttccggg
    60301 atgtttttac gcagccagag agcaaagatg attggcaaaa tgccgataaa gaacagcgca
    60361 cgccagcccc agaccggaac caccaggcta tagacctgag cggcaacgac ggcccccaca
    60421 gagaagcctg aaatcagaaa accactggct ttgttacgca gatgttttgg ccagctttca
    60481 atgacatagg tggcgctgga actgtattca cccgccatcc ccatgccgat gaccagacgc
    60541 gcgataaaca tggtgatgta gcctggcgca aagccgcagg ccagcgtccc ggccgagaag
    60601 agaacgatgc tggtgaccat tgccagacga cgcccgtagc ggtcacccat agcgccgagc
    60661 atcaggccgc cgaaccagcg agagataaag gctgcggaga tcagacttgc cgcctgcacc
    60721 gtcgtcagcc cgaattcacc ctgtacttcg gtgagtacca gggcgattaa aacgaaatca
    60781 aaaccgtcaa gcagatatcc caaccaggca gcggaaaatg cgcgccattg tgcacggttg
    60841 agatggcgat accacgggat attctgggtt gtagtactca ttgtgagtct cccgcggtgg
    60901 gcgatgccca cacgctttgg tataaaaatt gtagggtaca gatgcgttta tttcccctca
    60961 cccggtaggg gcgagcgagg ggaaacaact cacccgcgct cttgcatcaa ctgctgggcc
    61021 agcgccttca gttctggcag atatttttca tctaccggtc caaacggttt gcggcacagc
    61081 ggcacagaaa cgacatccat ataatggagg acagttttca ggccgcggaa tacgcccgtt
    61141 ttgatcagta aatcaatgac tttattgcat tcagtttgca gtttctgcgc ggtctggata
    61201 tcgccttctt tcagcgcctt aacgatcccc tgatagcgcc agcccatgat gttgtaggta
    61261 ctaccgatac caccatcagc gcccgccagc agaccagagg cgaagatttc gtcgtaaccg
    61321 ttatagagca caagatcagg atgttcacga cggatctgct ccatctgata gagatcgcca
    61381 gaggtctgtt tcagcgcacc tacgccaggc aatgtaacaa gtgtgttgat ctgatccagg
    61441 gtcagtttta ccccactcag ggctggaatg ttgtacacca ccatcggcaa accatccgct
    61501 gaatcaataa ttgcccgata gtgatcgcag tgttcttcaa agctgaaagg atagtagaac
    61561 ggcgtgacgg cggagacggc atcgaagcca taacgtttag ccgatgccgc aagttgttgg
    61621 ctttcggcgg tgctgacgca accgacgtgg gcgatgagtt taatcttacc tttcgcctct
    61681 tcggcgacga tttccagtac ctgttcacgc tcggaaaggc tttgtacaaa ggcctcgccg
    61741 gtcgaaccac ccacgtataa accgtcgatg ccctgctgaa tattgaactg aaccaggcga
    61801 cgcagactcg ctttatccag tgcttgttgt tgatcaaaag gagtcaggag tgcagccatt
    61861 acgccacgta aattcgttgc cataaatacc tctgaagtga tgcttgtctg ataaacgata
    61921 tacctttata cctgttatac cagatcaatt aagcaacacc ccatacagaa agcttataat
    61981 gcgatctgct tcactaaagt ggcattattt ctttttgttg gtggtctgac cgaaagcgtg
    62041 ccaggtagca gagacgctgt tgagatgcga ttgcaacgca cgatcggctt cgtcaggatc
    62101 atgacggcgg atcgcatcaa cgatcgcaat atgctgttga taactaacgt tgttatgttc
    62161 gtgcagtgcc tgatcggtaa ccgttgggcg tgcggcaata agccagtcga gcagggcaac
    62221 gtggatcgcc atgaagattg ggttaccggg gatctccgcc agcacgcggt ggaaatcaac
    62281 gtctgaacga atgaatgccg cgttgttatc cagcgactga ctgttaattt ccagtgcttt
    62341 tgccagcaaa tcgatttgct catcggtggc atgttcagcc gcatagcgca ccaggctgga
    62401 ttcaaagaac agacgtaatt gttcgaaatg ggcaatccca ccaggatgag aaaggaaatc
    62461 tttcgccatg ccggaaagct cgccgatgat ggtgtccgca gaaggacgtg agacgcgggc
    62521 acgttcgccg ttgttgattt gcaccagacc tttgcgtttt aacgctgcca gcgcttcgcg
    62581 cacagaagga cgcccgacgt taaagaacgc catcagttcg cgttcagacg gtaattgttc
    62641 accttcgcca aattcacgac ggcggatcat ctgttccagc tcttcttcca ccatttcgga
    62701 gagtttttta cgcgccagcg ggcggctacg caagttgcga ccaattgcag gtgaagaatc
    62761 ttcggtttgc gaatcaaatg cgttcataag gcccattctg taaggtcagt gtgattaaca
    62821 tcatcagtga catcctatca caggattgaa agtaggggaa aatggcaggt ttttctcttt
    62881 gtgcctcatc atta
//
LOCUS       DAZGSP010000013        62244 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZGSP010000013 DAZGSP010000000
VERSION     DAZGSP010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.013"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(170..712)
                     /gene="ycfP"
                     /locus_tag="ACHK74_000955"
     CDS             complement(170..712)
                     /gene="ycfP"
                     /locus_tag="ACHK74_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFP0219816.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(735..1760)
                     /gene="nagZ"
                     /locus_tag="ACHK74_000956"
     CDS             complement(735..1760)
                     /gene="nagZ"
                     /locus_tag="ACHK74_000956"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFP0219817.1"
                     /translation="MGPVMLDVKGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAEGVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(1771..2595)
                     /gene="thiK"
                     /locus_tag="ACHK74_000957"
     CDS             complement(1771..2595)
                     /gene="thiK"
                     /locus_tag="ACHK74_000957"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFP0219818.1"
                     /translation="MPFRSNNPITRDELLSRFFPQYHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2576..3217)
                     /gene="lpoB"
                     /locus_tag="ACHK74_000958"
     CDS             complement(2576..3217)
                     /gene="lpoB"
                     /locus_tag="ACHK74_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFP0219819.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTQVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3231..3608)
                     /gene="ycfL"
                     /locus_tag="ACHK74_000959"
     CDS             complement(3231..3608)
                     /gene="ycfL"
                     /locus_tag="ACHK74_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFP0219820.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3611..3970)
                     /gene="hinT"
                     /locus_tag="ACHK74_000960"
     CDS             complement(3611..3970)
                     /gene="hinT"
                     /locus_tag="ACHK74_000960"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFP0219821.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4304..6493
                     /gene="fhuE"
                     /locus_tag="ACHK74_000961"
     CDS             4304..6493
                     /gene="fhuE"
                     /locus_tag="ACHK74_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFP0219822.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGIYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGTPRNFSITGTYQF"
     gene            complement(6553..7986)
                     /gene="ptsG"
                     /locus_tag="ACHK74_000962"
     CDS             complement(6553..7986)
                     /gene="ptsG"
                     /locus_tag="ACHK74_000962"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFP0219823.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8281..9078)
                     /gene="ycfH"
                     /locus_tag="ACHK74_000963"
     CDS             complement(8281..9078)
                     /gene="ycfH"
                     /locus_tag="ACHK74_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0219824.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9089..10093)
                     /gene="holB"
                     /locus_tag="ACHK74_000964"
     CDS             complement(9089..10093)
                     /gene="holB"
                     /locus_tag="ACHK74_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFP0219825.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALCLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10090..10731)
                     /gene="tmk"
                     /locus_tag="ACHK74_000965"
     CDS             complement(10090..10731)
                     /gene="tmk"
                     /locus_tag="ACHK74_000965"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP0219826.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10721..11743)
                     /gene="yceG"
                     /locus_tag="ACHK74_000966"
     CDS             complement(10721..11743)
                     /gene="yceG"
                     /locus_tag="ACHK74_000966"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFP0219827.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11746..12555)
                     /gene="pabC"
                     /locus_tag="ACHK74_000967"
     CDS             complement(11746..12555)
                     /gene="pabC"
                     /locus_tag="ACHK74_000967"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFP0219828.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12675..13916)
                     /gene="fabF"
                     /locus_tag="ACHK74_000968"
     CDS             complement(12675..13916)
                     /gene="fabF"
                     /locus_tag="ACHK74_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFP0219829.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14004..14240)
                     /gene="acpP"
                     /locus_tag="ACHK74_000969"
     CDS             complement(14004..14240)
                     /gene="acpP"
                     /locus_tag="ACHK74_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP0219830.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14451..15185)
                     /gene="fabG"
                     /locus_tag="ACHK74_000970"
     CDS             complement(14451..15185)
                     /gene="fabG"
                     /locus_tag="ACHK74_000970"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFP0219831.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15198..16127)
                     /gene="fabD"
                     /locus_tag="ACHK74_000971"
     CDS             complement(15198..16127)
                     /gene="fabD"
                     /locus_tag="ACHK74_000971"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP0219832.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGKVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16143..17096)
                     /gene="fabH"
                     /locus_tag="ACHK74_000972"
     CDS             complement(16143..17096)
                     /gene="fabH"
                     /locus_tag="ACHK74_000972"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFP0219833.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSINLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17164..18234)
                     /gene="plsX"
                     /locus_tag="ACHK74_000973"
     CDS             complement(17164..18234)
                     /gene="plsX"
                     /locus_tag="ACHK74_000973"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFP0219834.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18315..18488)
                     /gene="rpmF"
                     /locus_tag="ACHK74_000974"
     CDS             complement(18315..18488)
                     /gene="rpmF"
                     /locus_tag="ACHK74_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP0219835.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18540..19061)
                     /gene="yceD"
                     /locus_tag="ACHK74_000975"
     CDS             complement(18540..19061)
                     /gene="yceD"
                     /locus_tag="ACHK74_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFP0219836.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19260..19844
                     /gene="yceF"
                     /locus_tag="ACHK74_000976"
     CDS             19260..19844
                     /gene="yceF"
                     /locus_tag="ACHK74_000976"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFP0219837.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(19956..20915)
                     /gene="rluC"
                     /locus_tag="ACHK74_000977"
     CDS             complement(19956..20915)
                     /gene="rluC"
                     /locus_tag="ACHK74_000977"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFP0219838.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21488..24673
                     /gene="rne"
                     /locus_tag="ACHK74_000978"
     CDS             21488..24673
                     /gene="rne"
                     /locus_tag="ACHK74_000978"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFP0219839.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(24870..25823)
                     /gene="flgL"
                     /locus_tag="ACHK74_000979"
     CDS             complement(24870..25823)
                     /gene="flgL"
                     /locus_tag="ACHK74_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFP0219840.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(25835..27478)
                     /gene="flgK"
                     /locus_tag="ACHK74_000980"
     CDS             complement(25835..27478)
                     /gene="flgK"
                     /locus_tag="ACHK74_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFP0219841.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27544..28485)
                     /gene="flgJ"
                     /locus_tag="ACHK74_000981"
     CDS             complement(27544..28485)
                     /gene="flgJ"
                     /locus_tag="ACHK74_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFP0219842.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28485..29582)
                     /gene="flgI"
                     /locus_tag="ACHK74_000982"
     CDS             complement(28485..29582)
                     /gene="flgI"
                     /locus_tag="ACHK74_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFP0219843.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29594..30292)
                     /gene="flgH"
                     /locus_tag="ACHK74_000983"
     CDS             complement(29594..30292)
                     /gene="flgH"
                     /locus_tag="ACHK74_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFP0219844.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30345..31127)
                     /gene="flgG"
                     /locus_tag="ACHK74_000984"
     CDS             complement(30345..31127)
                     /gene="flgG"
                     /locus_tag="ACHK74_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFP0219845.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31265..32020)
                     /gene="flgF"
                     /locus_tag="ACHK74_000985"
     CDS             complement(31265..32020)
                     /gene="flgF"
                     /locus_tag="ACHK74_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFP0219846.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32040..33245)
                     /gene="flgE"
                     /locus_tag="ACHK74_000986"
     CDS             complement(32040..33245)
                     /gene="flgE"
                     /locus_tag="ACHK74_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFP0219847.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYQATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(33270..33965)
                     /gene="flgD"
                     /locus_tag="ACHK74_000987"
     CDS             complement(33270..33965)
                     /gene="flgD"
                     /locus_tag="ACHK74_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFP0219848.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(33977..34381)
                     /gene="flgC"
                     /locus_tag="ACHK74_000988"
     CDS             complement(33977..34381)
                     /gene="flgC"
                     /locus_tag="ACHK74_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFP0219849.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34385..34801)
                     /gene="flgB"
                     /locus_tag="ACHK74_000989"
     CDS             complement(34385..34801)
                     /gene="flgB"
                     /locus_tag="ACHK74_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFP0219850.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            34956..35615
                     /gene="flgA"
                     /locus_tag="ACHK74_000990"
     CDS             34956..35615
                     /gene="flgA"
                     /locus_tag="ACHK74_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFP0219851.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            35691..35984
                     /gene="flgM"
                     /locus_tag="ACHK74_000991"
     CDS             35691..35984
                     /gene="flgM"
                     /locus_tag="ACHK74_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFP0219852.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            35989..36405
                     /gene="flgN"
                     /locus_tag="ACHK74_000992"
     CDS             35989..36405
                     /gene="flgN"
                     /locus_tag="ACHK74_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFP0219853.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(36445..37980)
                     /gene="murJ"
                     /locus_tag="ACHK74_000993"
     CDS             complement(36445..37980)
                     /gene="murJ"
                     /locus_tag="ACHK74_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFP0219854.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RMV"
     gene            complement(38090..39013)
                     /gene="yceM"
                     /locus_tag="ACHK74_000994"
     CDS             complement(38090..39013)
                     /gene="yceM"
                     /locus_tag="ACHK74_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP0219855.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39015..39662)
                     /gene="yceH"
                     /locus_tag="ACHK74_000995"
     CDS             complement(39015..39662)
                     /gene="yceH"
                     /locus_tag="ACHK74_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFP0219856.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39673..40257)
                     /gene="rimJ"
                     /locus_tag="ACHK74_000996"
     CDS             complement(39673..40257)
                     /gene="rimJ"
                     /locus_tag="ACHK74_000996"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFP0219857.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40493..41701
                     /gene="mdtH"
                     /locus_tag="ACHK74_000997"
     CDS             40493..41701
                     /gene="mdtH"
                     /locus_tag="ACHK74_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFP0219858.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41765..42412
                     /gene="grxB"
                     /locus_tag="ACHK74_000998"
     CDS             41765..42412
                     /gene="grxB"
                     /locus_tag="ACHK74_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFP0219859.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42546..43106
                     /gene="yceB"
                     /locus_tag="ACHK74_000999"
     CDS             42546..43106
                     /gene="yceB"
                     /locus_tag="ACHK74_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0219860.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43212..44258
                     /gene="pyrC"
                     /locus_tag="ACHK74_001000"
     CDS             43212..44258
                     /gene="pyrC"
                     /locus_tag="ACHK74_001000"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFP0219861.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44332..44577
                     /gene="dinI"
                     /locus_tag="ACHK74_001001"
     CDS             44332..44577
                     /gene="dinI"
                     /locus_tag="ACHK74_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFP0219862.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            44867..45121
                     /gene="bssS"
                     /locus_tag="ACHK74_001002"
     CDS             44867..45121
                     /gene="bssS"
                     /locus_tag="ACHK74_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFP0219863.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            45236..46354
                     /gene="solA"
                     /locus_tag="ACHK74_001003"
     CDS             45236..46354
                     /gene="solA"
                     /locus_tag="ACHK74_001003"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFP0219864.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIHHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            46402..46515
                     /locus_tag="ACHK74_001004"
     CDS             46402..46515
                     /locus_tag="ACHK74_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFP0219865.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            46776..47342
                     /gene="yceJ"
                     /locus_tag="ACHK74_001005"
     CDS             46776..47342
                     /gene="yceJ"
                     /locus_tag="ACHK74_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFP0219866.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            47346..47921
                     /gene="yceI"
                     /locus_tag="ACHK74_001006"
     CDS             47346..47921
                     /gene="yceI"
                     /locus_tag="ACHK74_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFP0219867.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(47963..49015)
                     /gene="trhO"
                     /locus_tag="ACHK74_001007"
     CDS             complement(47963..49015)
                     /gene="trhO"
                     /locus_tag="ACHK74_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFP0219868.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            49240..50160
                     /gene="lpxL"
                     /locus_tag="ACHK74_001008"
     CDS             49240..50160
                     /gene="lpxL"
                     /locus_tag="ACHK74_001008"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFP0219869.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            50332..51558
                     /gene="mdtG"
                     /locus_tag="ACHK74_001009"
     CDS             50332..51558
                     /gene="mdtG"
                     /locus_tag="ACHK74_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFP0219870.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            51641..52015
                     /gene="msyB"
                     /locus_tag="ACHK74_001010"
     CDS             51641..52015
                     /gene="msyB"
                     /locus_tag="ACHK74_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP0219871.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(52016..52243)
                     /gene="yceK"
                     /locus_tag="ACHK74_001011"
     CDS             complement(52016..52243)
                     /gene="yceK"
                     /locus_tag="ACHK74_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP0219872.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(52416..54959)
                     /gene="mdoH"
                     /locus_tag="ACHK74_001012"
     CDS             complement(52416..54959)
                     /gene="mdoH"
                     /locus_tag="ACHK74_001012"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFP0219873.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(54952..56487)
                     /gene="mdoG"
                     /locus_tag="ACHK74_001013"
     CDS             complement(54952..56487)
                     /gene="mdoG"
                     /locus_tag="ACHK74_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFP0219874.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            56881..58038
                     /gene="mdoC"
                     /locus_tag="ACHK74_001014"
     CDS             56881..58038
                     /gene="mdoC"
                     /locus_tag="ACHK74_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFP0219875.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(58046..59527)
                     /gene="clsC"
                     /locus_tag="ACHK74_001015"
     CDS             complement(58046..59527)
                     /gene="clsC"
                     /locus_tag="ACHK74_001015"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFP0219876.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(59469..60002)
                     /gene="ymdB"
                     /locus_tag="ACHK74_001016"
     CDS             complement(59469..60002)
                     /gene="ymdB"
                     /locus_tag="ACHK74_001016"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFP0219877.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(60097..60408)
                     /gene="ymdA"
                     /locus_tag="ACHK74_001017"
     CDS             complement(60097..60408)
                     /gene="ymdA"
                     /locus_tag="ACHK74_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFP0219878.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(60529..60861)
                     /gene="csgC"
                     /locus_tag="ACHK74_001018"
     CDS             complement(60529..60861)
                     /gene="csgC"
                     /locus_tag="ACHK74_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFP0219879.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(60920..61375)
                     /gene="csgA"
                     /locus_tag="ACHK74_001019"
     CDS             complement(60920..61375)
                     /gene="csgA"
                     /locus_tag="ACHK74_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFP0219880.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(61416..61871)
                     /gene="csgB"
                     /locus_tag="ACHK74_001020"
     CDS             complement(61416..61871)
                     /gene="csgB"
                     /locus_tag="ACHK74_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFP0219881.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            complement(62000..>62244)
                     /locus_tag="ACHK74_001021"
     CDS             complement(62000..>62244)
                     /locus_tag="ACHK74_001021"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219882.1"
                     /translation="FFSAPPWTIFYCKKTRFVTACAQDISQQSATGKKNDCLVIFDSG
                     KRRFKRKQNIFCVDNSVFTWVLILWYEQKKKNTTRG"
BASE COUNT        15180 a        16567 c        15287 g        15210 t
ORIGIN      
        1 aatatagggt gaagcgtata tcgagggcgt ttggtcgtcc tgataagatg cgcagcatcg
       61 catcaggcat tgatgctcga ctgtcggatg cggcgtggac gccttatccg acttacagaa
      121 ttcggagcgc atcaggtacc ggtaaggtta cctgatgcga cgagggcatt tacccgaggg
      181 ttttgaacgc tttaatgcgc tgtaaatgcg gggagatatt cttgaatttg tgcgtctgct
      241 cttcgtccca gacaatctcg taataatgat gcaactcttc agatgtccgc tggctgttaa
      301 gtgcttcatc attacgcgac aaaataacca gacaacgatc gcgattcttc tcgcggaagt
      361 tggtcacaca cttagtggca atatcggcat actcttccgg gcgatcaatc ttgccttcca
      421 tgttctcata agggaacaaa ttagggttga agatcacctg gcggatatcg caaagaaaac
      481 caatccgttc cgcccagtat ccgcccaggc caacgccgca aattagcgga cgctcgtcaa
      541 cgttcagttg caacattttg tccacttctt taagcagatg ctgcatatca tgtttcggat
      601 gccgcgtact gtagcttatc aagcgtacat ccgggtcaat aaactgcaat tgtaagactt
      661 tctcgtggtt acccggactg ttagagtcaa aaccgtgtaa atagataatc atcgcttcct
      721 cacataagcc agggttagtg acctgctttc tcttcctgcc agcgttcgtg taactgattc
      781 agacgggtgc tgatcgcttt ccagcgagcc gagtccatca gttcctgtcg cgaaaatgaa
      841 cctttatgat acaaacgtgt aacaccttct gccttgatcg gtgacagatt atctaacacg
      901 cttacggccc ctttacgatt attgcagacc aggatcatat cgcaacccgc atccagtgat
      961 gcctgcccgc gttcggcata actgcccata atcgcggcac cttccatcga taaatcgtca
     1021 gagaatatca cgccgtcaaa acccagttcc tgacgcaaaa cggttttcag ccagtaggga
     1081 gaaccgctcg ccggacgcgg atcaacatca ctgtagatca catgcgcagg cataatggcg
     1141 tcgagtttat tttcgcggat taaggaactg aagaccgaca tatctttagc gcgaatctcc
     1201 gcttgtggac gtggatcgca cggtgtttct ttgtgtgagt ctgccgttac tgcaccgtgt
     1261 cctgggaagt gtttcccggt cgttttcatt ccggcttcat gcataccatc aataaaccgg
     1321 ctggcaattg ccagggcttt ttgtggatcg gcatgataag aacgctcgcc aatcgccgcg
     1381 ctgatatgcc cgacatccag cactggcgca aagctgatat caatatccat agcgatcatt
     1441 tcgctggcca tcagccaacc cgcctcttgc gccagtttgc cgccctcttc cattcctgac
     1501 agcgcagcga atgattgcgc cgctggcaag cgggtaaaac cttcacgaaa acgctgcacg
     1561 cgtccacctt cctgatctac cgccaccacc agatgattgc gcgaagctgc gcggatctgg
     1621 cgcaccagtt cacgtaactg ggcaggatca tgatagttac gcgtaaagag aatcagccct
     1681 cccaccagcg gatgcgcaag tatttcacgc tcttccgcgt ccagttcgta acctttgaca
     1741 tccaacatta ctggacccac actgctctcc ttattgtttt attaatagct gccgccaggt
     1801 gtcatcggcc agcctgataa attgttgatc gccggtttgt cgccagcggt actcaaacca
     1861 ccctgctttg agcatcagca gccagggaaa ccatcgcctg acctgacgcc ataattgcgc
     1921 tggataaatc ttcgcgcgag tggcatagtc attgaccaat tgccggtgct gttcagtatt
     1981 ttccacccac accgccgcca gttccagcgc gatatcacca tctccggcat actcccagtc
     2041 gatgagtttt aaccctgacg cgctatgcac taaatttccg gcgtggacat ccatatgcaa
     2101 tggacttaag cgtaaaggcc gtggttcccg cgctttgcgc agacgtttta acattcgcag
     2161 ccaacccact gtccgccgcg ccggatcgct ttgctgccag tacagttcca gtaacggcaa
     2221 cagcgttatt cgccagccaa aacgtggttg ttgatgtaga taatacagca agcctgccag
     2281 ttcgttggta tccggcaaat acgtttttac cgcgccgggc agatagtcga ctaccatcca
     2341 gtcacggaga tataaatgcg gcttcggtgc aatgcttgcg ggtagttgtg ataaagcccg
     2401 atactggcgc aagaacgcgg actgcggcgc atcaggatcg tgcggctgac gcacaacaaa
     2461 acgctggccc tgatgttcaa tgagaaaact cccgccacta agcccactat taaacgtcgt
     2521 gacgggatga tactgcggga aaaagcgcga cagcaattcg tcgcgcgtga tgggattatt
     2581 gctgcgaaac ggcaccttta cctgaccaga taatttcgcc cgtctgcacc agcatcagct
     2641 gcatttgtag ggtcggagcg ttaacgttgc cagaggcgct ggagtacagc acgtaatgag
     2701 cgccgacatt gcgggcaatg cctatggctt tactacgggt gcctaaactg tcctgcggcg
     2761 acaaacctaa ctgttgctta gccatcgaca gttgctgggc ggaaacctgg gtaaatttcc
     2821 cgttattagc cagcgcattt cgcagcgttt cggtcgcttc tgcggcattc agcgaaccgt
     2881 tagtacggtt gttaacgcta tcaaccagca ggacgctacc cgcagtcacc ccgtcagccc
     2941 caagcatctt acttaccatc ggctgcattg cgccattcca gtcataatgg cgaatatgcg
     3001 gcgcaggcgg tgcagtttga tcttcgtgct caattgggcc tggctgctgc gggatcgtcg
     3061 gcaccgaggg cactgtgggg acaggctgtt gtggctcggc tggttgttcc ggcgctggtt
     3121 tcacttcttc taccggtgca ggttcacgtt gccccacaca cccggcgaga aacatcgcca
     3181 gcgcggtaat caaggcgtag cgactcattt ttgtcatcaa gattcacccc ttacaaatat
     3241 agataaagtc tgactttgtg cgcccccaga aaattggcgc tgccgtacag cgttaccgcc
     3301 gaatgtgcgg gaatgctaac gctgcgtggc ctttccagag gatgcatctc cagccctctg
     3361 gcgtcatacc agtaaaaacg ataatgaacg gtaacgggtt cttgcctttc gttataaagc
     3421 gttgaggatg ctgaaggttg aatatcagac gtcgaaagga cgggcttttc tgcactgatg
     3481 cccgcagcca ataaagatga ctccatcacc agcgattgct catcattcac cggaatttcc
     3541 ggatgtgaac gacagcccac cagcagtaac aacgccagag acaccagccc aaagcatcct
     3601 tttctcatcg ttacagacct ttatgcgcca gcattggtcc cagcggacgg ccacccaaca
     3661 agtgcatatg gatgtggtaa acctcttgtc cgccatggcg gttggtattc atgatcagac
     3721 gatagccatc ttcggcaata ccttcttgct cagcaatttt tgccgctacg gtgatcatgc
     3781 gtcccagcgc ctgctcatgc tcagctgaga cgtcgttcac agtcggaatg aggatattcg
     3841 gaatgatcag aatatgcgtt ggcgcttgcg gcgaaatatc gcgaaacgcc gttaccagat
     3901 catcctggta gacgatatcg gaggggatct cacgacgaat aattttgctg aatatagttt
     3961 cttctgccac gacattttcc tttttcaaat agccatgcgt tgcgccatgc tacgccgcgc
     4021 cacactgcga gtatagagta tggtcgagtt aaacctgctc tttcaacctt aactcacgat
     4081 ttcttaagca aaaaatcgtt ttgctgccgt atatatcgcc attattccca tttctgcctg
     4141 cgaatgcgct gaaaagacat tctctgtttg gtttacacga caaatatgaa tgcgtatatt
     4201 tctcatttgc atttacaaac aaaattattc gcacactaaa tataatcgct catcaccctc
     4261 ctccggcatg agcctgacga caacataaac caagagattt caaatgcttt caacacaatt
     4321 taacagggat aatcaatatc aagccatcac caaaccgtca ctacttgccg gttgcatagc
     4381 actggcacta ttaccttctg ccgcttttgc tgcaccagcc actgaagaaa cggtgattgt
     4441 tgagggttca gccacagctc cagatgatgg cgaaaatgat tacagcgtaa cgtctacctc
     4501 tgcgggtacc aaaatgcaga tgactcaacg tgatattcct cagtcggtca ctattgttag
     4561 ccagcagcgg atggaagatc agcagttaca aacgctgggc gaagtgatgg aaaacacgct
     4621 ggggatcagc aaaagtcagg cggattccga tcgtgctctt tattattccc gcggattcca
     4681 gatcgataac tatatggttg atggtatccc cacctatttt gaatcgcgct ggaatctggg
     4741 cgacgcactt tctgatatgg cactctttga acgcgtagaa gtagtgcgtg gcgcgacagg
     4801 actcatgacc gggacgggta atccatctgc ggcaattaat atggttcgaa aacacgcgac
     4861 cagtcgtgaa tttaaaggcg atgtctcggc ggaatacggt agctggaaca aagaacggta
     4921 tgtggcggat ttacaaagcc cactcaccga agacggtaaa atccgcgcgc gaattgtcgg
     4981 cggctaccag aataacgact catggctgga ccgctacaac agtgaaaaga ccttcttctc
     5041 gggcattgtc gatgctgatt taggcgatct tacgacgctt tcagccggtt acgaatatca
     5101 gcgcattgat gttaatagcc ctacctgggg cggtttaccg cgctggaata ctgatggcag
     5161 cagcaacagt tacgatcgtg cacgcagtac cgcacctgac tgggcgtaca acgataaaga
     5221 gatcaacaag gtctttatga ccctgaagca gcggtttgct gatacctggc aagcgacact
     5281 gaatgccacc cactctgaag tcgaatttga cagcaaaatg atgtatgtcg atgcctatgt
     5341 aaacaaagcg gatggtatgc tggttgggcc atacagtaat tatggacctg gctttgatta
     5401 tgtcggcggc accggttgga acagtggcaa acgtaaagtt gatgcgctgg atttgttcgc
     5461 tgacggtatt tatgaattgt ttggtcgtca gcacaatcta atgtttggtg gcagttacag
     5521 caaacaaaac aatcgttact tcagttcatg ggccaacatc ttcccggatg aaattggcag
     5581 tttctacaac tttaatggca atttcccaca aaccgactgg tcaccacaga gcctggcaca
     5641 ggacgatacc acacatatga aatcgttata tgctgccact cgagtcaccc ttgccgatcc
     5701 gctgcatctg atcctcggcg cacgttatac caactggcgg gttgatacgc tgacttacag
     5761 catggagaaa aaccacacca cgccttacgc tggtctggtg tttgacatca atgacaactg
     5821 gtcgacctac gccagctata cctctatttt ccagccgcaa aatgatcgtg acagttcagg
     5881 caaatatctg gctccaatca ccggtaacaa ctacgagctg ggtctgaaat cggactggat
     5941 gaatagccgt ctgaccacca cgttagccat cttccgtatt gagcaggata atgtcgctca
     6001 gtccaccggt acacctatcc ccggcagcaa cggcgaaacc gcctataaag cggtggatgg
     6061 gacagtcagt aaaggggtgg aatttgaact caacggcgca attaccgaca actggcagct
     6121 gacatttggc gcaacgcgct atattgcaga ggataacgaa ggaaacgccg ttaatcctaa
     6181 tctgccacgc accacggtta aaatgttcac cagctatcgg ttgcctgtca tgccagagtt
     6241 gacagtcggc ggtggtgtta actggcaaaa tcgcgtgtat accgacaccg tgacaccgta
     6301 tggcaccttc cgcgccgagc aaggtagcta cgcgctggtg gatctcttca cccgctacca
     6361 ggtgacgaaa aacttctcgt tacaggggaa cgtcaataac ctgttcgaca aaacctacga
     6421 taccaacgtg gaaggttcta tcgtctacgg cacaccgcgt aatttcagca ttaccggcac
     6481 gtatcaattc tgaataacac ctgtaaaaaa ggcagccatc tggctgcctt agtctcccca
     6541 acgtcttacg gattagtggt tacggatgta ctcatccatc tcggttttca ggttatcgga
     6601 tttagtaccg aaaatcgcct gaacaccaga accagcaacc actacgcccg ctgcgcccag
     6661 tttcttcagg ccggcctgat ccactttaga cacatcagca acgctgacgc gcagacgggt
     6721 aatacatgcg tcgaggttag taatgttttc tttaccacca aatgcagcaa ccagagccgg
     6781 tgccatttcg ctggtacctg tcgcttttgc atcttcagtc gcgtcttcac gacccggcgt
     6841 tttcagatcc agtgctttaa tcagcacgcg gaagatggtg tagtaaacaa tcgcataacc
     6901 gataccgacg atcgggaaca gccacagttt gctgctgtta ccagacagaa cgatgaagtc
     6961 gatcagaccg tgcgagaacg acgtaccgtc acgcatcccc agaagaatac agattgggaa
     7021 tgccaggcct gccagaatcg cgtggatgat gtacaggatc ggcgcaacga acatgaagga
     7081 gaactcgatc ggctcggtga taccggtcag gaacgaggtc agcgccgcgg agatcataat
     7141 accgcccact ttcgcgcggt tttctggttt agcagagtgc cagatagcaa ttgcggcagc
     7201 tggcagaccg tacattttga acaggaagcc accagacagt ttacccgcag tcgggtcacc
     7261 cgccatataa cgcggaatgt cgccgtggaa aacctgacct gctgcgttgg tgtattcacc
     7321 aatctgcatc tggaaaggta cgttccagat gtggtgcaga ccaaacggta ccaggcaacg
     7381 ttcgatgaaa ccgtaaatgc caaacgcaac aaccgggttc tggtaagcag cccactgaga
     7441 gaaggtctgg attgcagaac caatcggcgg ccaaatgaag gacagcacaa cgccagtaaa
     7501 gatggcagcc aggccagaaa tgatcggcac aaagcgttta ccggcaaaga agccaagata
     7561 ctcaggcagc ttaatacggt agaaacggtt aaacatgtac gctgcgatcg caccggagat
     7621 aatccctccg agtacgccag tatccgccag gtgtttagag gcgatttctt cagcaggtaa
     7681 atgcagtacc agtggcgcaa ccacggccat ggttttaacc atgatgccat aggcaacaac
     7741 tgcggccagc gcggatacgc catcgttatt ggtaaagccg agggcgacac cgatcgcaaa
     7801 aatcagtggc atgtttgcaa agacggaacc gcctgcttct gccataacat gcgatacaac
     7861 ggcgggcagc cagctgaaat tcgcggaacc gacgcccagc agaatacctg cgataggcag
     7921 tacggatacc ggcagcatca gcgatttacc gaccttttgc aggttagcaa atgcattctt
     7981 aaacataatt gagagtgctc ctgagtatgg gtgctttttt tacgttctca cgcgtggcaa
     8041 ggggggagag cctcgccgtg tacagggcat ctaagcgccc tttatttatt acacagagta
     8101 aaataattca gtgccaattt gtttgacggc tatcacgttt caacttcaga tcattggaga
     8161 agttgcacat tttgacaaaa catatgtcac aatatttcaa acgtggttat tcaccgccca
     8221 attttcggcg gtgaacttta ctcgttttag ccattaatta cgagctttaa aaaaactcat
     8281 tcaacggatg gattgaaggc gggaagcgtc gatgtgaaac agacgggcga agttatcggt
     8341 ggttacctgc gccagttctt caacggcaac acctttcaac acagccatgt attctgcaac
     8401 gtcacgaacc atcgcaggtt gattctcttt tcctcgatgc ggtaccggcg caaggtaagg
     8461 tgagtcagtt tccaccagta atcgatccag ggggacataa cgcgcagcat cgcgcagttg
     8521 ctccgcatta cggaaggtca caatgccgga aaaggagatg taaaatccga gatccagtaa
     8581 tttacccgcc gtttctctgt cctctgtaaa acagtgtagt acgccaccgc aatccgtcac
     8641 tttttcttcg cgcagaattg ccagcgtatc ggcacgggcg tcacgggtat ggacgataac
     8701 cggcttgttc agttcacgac caatctggat atgatggatg aaggactctt gctgacgtac
     8761 tttagtttcc ggtgtgtaat aataatccag cccggtttca cccagcgcta caacaccctc
     8821 ttctgccgcc agacggcgta aatcttctac atcgtaggga tcattctggt tgagtggatg
     8881 cacgccacaa gaaaatacga cgttgtcacg ttcgcctacc agatcccgca tatgtaaata
     8941 acccggtaat gttgtggcga ctgccagaca aaatttcaca tcgcgtgcgg cggctttcgc
     9001 cagaacgtca tccacgtcct tatgcaaaga ttcataatcc agaccatcga gatggcagtg
     9061 tgagtcgact aaaaacatga tgtctctctt aaagatgagg aaccggtagc acaacgcccg
     9121 gttgcaggta atgctcaata cgcagtaaaa gatcggtgat gagaagctcg cggttgatgc
     9181 ctgtgacaga cattaactgt tcacgaatgt ggcaaacatc ccccagtata gcctgcaggc
     9241 gcgagggaga aagatggttt gccagttcgg cgaccaggcc cggcacatca acattggtca
     9301 cctgcgcagc gccatgatgg cgttttagcg catccatcag caacgttgcc agccagtgta
     9361 aacgcgccgg agcttgttca tgattaaggg ccgccagcag cgaataccag tcgcccgatg
     9421 gcacgctata tgccaacgcc tgacacaatg tttcacgagc ctgccagtta tctccctgaa
     9481 acaacgccag tgccgcgcca ggcgaaccgg cgcttaagca caatgcggca agtaatgcat
     9541 cctgtgacat tgtcacttcg cgtgaaagcc aggtcacggc gtactgttcc ggcggcggcg
     9601 caaggtaatg taaccgacaa cgactacgta atgttgccag taaacgttca ggctcgcggg
     9661 tagccaggaa aaaccaggtt tctgctggtg gctcttcaag cgttttcagc aatgcgttag
     9721 ccgcggcgtc ggtcagtaag gcagcatcgg ttacccagac aactttcgca ccacctaagc
     9781 gtgcgtgctc attcagcttt tcggtgacct cacgtaccgc atcaatgccc aacgcatttt
     9841 tccctttttc gggagccagg gtgtagtaat cgggatgcgt gccagcctgc atcaactgac
     9901 atccacgaca gtgaccgcaa cttttgtgac cctgcggttg ttggcagagt aaataacggc
     9961 tcagggcgta gattaaagca tcatcgccca tgcccggtaa cgcctgaatg agcagcgcat
    10021 ggtgacctct tccggcctga tagctggcta ccagtttttc gaaatcaggt cgtaaccatg
    10081 gataccatct catgcgtcca actccttcac ccagtgggtc acggtagtgc ggattgcatc
    10141 catcacggcc tccagcggct gggtggcatc aatggtatga atgcttttat cttgtgctgc
    10201 cagttccaga tagcgggcgc gggtgcgatt aaagaaatcg aaagactctt gctcaatacg
    10261 atccagctcg ccgcgcgcac gcgcgcgttt taagccaact tccggggtaa catcgagata
    10321 gagcgttaag tcggggcgaa aatccccgag aacagcatca cgcagtgttg ccagcatatg
    10381 ttggtcaata ccacgtcctc cgccctgata cgcctgagtg gagagatcgt ggcgatcgcc
    10441 aatcacccag gtgccgttag ccagcgcagg tttgatgact gtttctacca gttgaacgcg
    10501 cgcggcataa aacatcagaa cttcggcttt atcggtaatg acttcatcgc ctaccgattt
    10561 gatatccagc accaggcttc ttaacttttc ggcaagttgc gtaccgccag gttcccgagt
    10621 gaaaaccatg tcgcggatac ccagttgctc gagcgtctca accaccacat tacgcgcggt
    10681 agttttgcct gcgccttcca gcccctcaat gacgatatac ttactgcgca tttttttcct
    10741 taagcacttt cagataatcc tgcacagact tgttatgact ggcaagattg gtattaaacg
    10801 tgtgaccacc tttaccatcg gccacaaaat agagatacgg cgtttttgcc ggatgcgcag
    10861 cagccttcag cgaatccgcc cccggcgtag ctatcgcacc cggcggcaaa ccggtaatgg
    10921 tataggtgtt atacgctgtc ggcgtttcca ggtctgcacg agaaagtttg ccattataac
    10981 gctctcccat cccgtaaatc acggttgggt cggtctgcag gcgcatacca atgcgtaaac
    11041 ggttgataaa taccgaggca acctgatcgc gttcactggc aacggcggtt tctttttcga
    11101 taattgatgc catcgtcacc agctgatttt tatctttata aggcagaccg tccgcacgcc
    11161 cttcccaggc gctatcgacc gctttcacca ttttcttgtg cgctcgcttg agtaacgcga
    11221 catcggtggt attggcggta tacatccagg tgtctggcca gaaccaacct tcaatccact
    11281 ccgggttttc cagttcaagt gcctgcgcta cggtggcgta cttatcgtca ctcagcgtgt
    11341 gcttgatata cggagcctca cgcaattgct tgaggtagtc gctcagacgc atcccttcta
    11401 ccagtcgcag cgggaactgt gcttctttac cgctttccaa caatttcagc atctcgcgca
    11461 cggtcatctg cggtgtaaag cggtaagtcc cggctttaaa gtgagaaaga tccggttcga
    11521 tacgcagcag ccattgaaaa acccgtgggc gattgatgat cttatcagca taaagctgtt
    11581 caccgagcgc cagacgtccg gtccctggct tcagggtaaa tatcgtctct tctttgataa
    11641 gcaatttgct gtcggcaaga tggcgaacct tccagacgcc cacaccagcg gcgataccca
    11701 gtaccaccag caataacaag attatcaata acactttttt catgactaat tcgggcgctc
    11761 acaaagtggg gctaaatatt catataacgt tgctgacgaa aaggagacat cgccacaggc
    11821 acgtacgggc atcactggca ttaacgcatt acaaataacc atctcatctg cctgcaacgc
    11881 ctcttccaga gaagcttgca cttcgacaag ctgataagag gattgtgcca gcaaacggat
    11941 acagaattgt cgcataatgc cgttaacacc tgcctgatcc agtcgcggcg tataaactac
    12001 gttgcccttc cgccagaaca aattagccgc acagcattcc gtaacccacc cttcgctgtc
    12061 aaggaccagc gcctcatcag cgtttgtctg ctcaagatga gagcggatca atacttgctc
    12121 aagccgatta agatgtttaa tacctgcaag atgaggattg cgccccagcc gcaccgggct
    12181 tagcgccaac gtcatccctt cgttacgcaa acggtcgtaa tgtgcaggat aaaccgtaac
    12241 ggagagaatc cgcgttgccg gtccactgtt caatgtgctg taccctcgcc cgccactacc
    12301 gcgactgatc acgactttca gcacaccatt ttgctgttct gctgccagcg ttttcatctc
    12361 ttgttcaagc tgaggccaga agtcacagaa aatcatcaac cgctgacaag catcctgtag
    12421 tcgctggata tgcgccgata acaaactgac tttaccgtcg ataactctgg cggtggtaaa
    12481 acaaccatca ccaaactgcg ttgcccgatc gcttactgcc agcgattcct gcttataacc
    12541 gttaattaag aacataccgg ctccttatca gagaattgtt agtgtggcag catgttcact
    12601 acggaacaag tcggaataaa agctaagaaa aaaggcccgc aagcggacct tttatatggg
    12661 tggaaaatga caacttagat ctttttaaag atcaaagaac cattagtgcc accgaagccg
    12721 aaggagttac acagcgtgta ttccattccg ctgacctgac gtgcttcatg cggtacgaaa
    12781 tccagatcgc aaccttcatc cgggttatcc aggttgatgg ttggcggaac agcctgatcg
    12841 cgcagcgcca ggatggagta gatagattct actgcacccg ccgcacctaa caggtgaccg
    12901 gtcatagatt tcgtggagct taccaacaca cggcttgcag cttcaccgaa gatggttttc
    12961 accgcctgcg cttcagcttt atcgccagcc ggcgtagaag taccgtgcgc gttaacgtag
    13021 ccaatctgac tcgcttcaat gcctgcatca cgcagagcat ttgccattgc cagagctgcg
    13081 cctgcgccat tttctggcgg tgacgtcata tgataagcat cgctgctcat accaaagccg
    13141 acgagttcag cgtaaatttt cgcaccgcgt tttttcgcgt gttcgtactc ttcaagtacc
    13201 agcataccgg caccatcgcc tagtacgaag ccatcacgct ctttatccca cgggcggctc
    13261 gccgcttgcg ggttatcatt gcgggtagat aatgcacgtg ccgcgccaaa accaccaacg
    13321 cccagcggcg tactggcttt ctctgcgcca cctgcaacca tcacgtcagc atcgccatac
    13381 gcgataatac gcgcagcatg accaatgttg tgcacgccgg aagtacaggc ggtcgcgata
    13441 gagatgcttg ggccacgcag gccatacatg atagtcagat gacctgccac catgttcaca
    13501 atcgttgacg gaacgaagaa tgggctgatc ttacgtggac caccgttcat cagagatgtg
    13561 tggttttctt cgatcagtcc gaggccgcca atcccggagc caattgcggc accaatgcgg
    13621 gttgcgttct cttccgttat ttcaaggcca gaatcctgca tggcctgaac gccagcgaca
    13681 attccatatt gaatgaaggc atccatcttg cgctgttctt tgcgcgagat aatgtcctca
    13741 cagttaaaat cctttactaa gccagcaaat ttcgttgcat aggcgctagt atcgaaatgg
    13801 tcgattaggc tgatgccact ctgaccggca agcagagctt tccaggtaga ctctacggta
    13861 ttgccgacag gagacaacat gcccagtccg gtcacaacta cacgacgctt agacacgttt
    13921 gtcctccagg gagggaaaaa atgattctag tgggacaaaa agataaaact caggcggtcg
    13981 aacgaccgcc tggagatgtt cacttacgcc tggtggccgt tgatgtaatc aatggcagcc
    14041 tgaacggtgg tgattttctc agcttcttcg tccggaatct cagtatcaaa ctcttcttcc
    14101 agagccatta ccagctcaac ggtgtcaaga gaatccgcgc ccaggtcttc aacgaaagaa
    14161 gcattgttgg taacttcttc ctgcttaacg cccagctgtt cgccgataat tttcttaacg
    14221 cgttcttcga tagtgctcat actcttaaat ttcctatcaa aactcgcttt cgcgatggtt
    14281 ttcgtagtgt ataaaatgtt gaaaaatttg caactaaatc ccggcaggtc ttaccacgat
    14341 tttacgttat tttgaggcct taccccccaa taacgcaaat cattttgcac taattattac
    14401 tcataaccac gcagactgcg cgcaacatga gcattttgtg caaatcgcgg tcagaccatg
    14461 tacatcccgc cgttcacatg caaagtttca cccgtgatgt aagctgcttc gtcggatgcc
    14521 aggaatgcaa ccgcgttggc gatttcctgt gcgccgccga ggcgacccgc aggaacctgc
    14581 gccaggatac ccgcacgctg gtcatcgctc agcgcacgtg tcatgtccgt ttcaataaag
    14641 cccggagcaa caacgtttac agtaataccg cgtgacgcaa cttcgcgcgc cagtgattta
    14701 ctgaagccga tcaagcccgc tttcgccgca gcgtagttgg cctgaccgcc atttcccatg
    14761 gtaccaacca cagaaccgat agtgataata cgaccatgac gctttttcat catagcgcgc
    14821 attaccgctt ttgacagacg gaaaacagat gaaaggttgg tttcgataat atcgttccac
    14881 tcttcatctt tcattcgcat taacaggtta tcacgagtga taccggcatt attgaccagg
    14941 atatccactt caccaaattc tgcgcgaatt ttttccagaa cagattcgat agatgccggg
    15001 tcggtcacat tcaacatcag acctttgccg ttagcaccta aataatcact gatcgcctga
    15061 gcgccatttt cactggtcgc agtgccaata actttcgcgc cacgggctgc gagcgtttca
    15121 gcaattgcgc ggccaattcc gcggcttgca ccggttacca gtgcgatttt tccttcaaaa
    15181 ttcatgattt tcctctttta aagctcgagc gccgctgcca tcgctgaagg ttcgttcagc
    15241 gccgaggcgg tcagggtgtc gacaatgcgt ttcgtcaggc cagttagcac tttgcccggg
    15301 ccgacttcat agagatgttc tacgccttgc gctgccatgt actcaacaga cttcgtccac
    15361 tgaaccgggt tatacaactg acgtaccagt gcgtcacgaa tggcatcacc attggtttcg
    15421 catttcacat caacgttatt cacaacagga actgttggtg cgttaaaggt gattttcgct
    15481 aattctactg ccagtttgtc ggctgctggt ttcatcagcg cacagtgaga cggtacgctc
    15541 actggtaacg gcagcgcgcg ttttgcgccc gcagctttac aggcagcgcc agcacgctca
    15601 accgcttctt tatgaccggc aataaccacc tgtcccggag agttaaagtt taccggagaa
    15661 acgaccttac cttctgcagc ttcttcacac gctttcgcaa tagacgcatc atccagaccg
    15721 atgattgccg ccatagcgcc agtgccttcc ggtacggctt cttgcatgaa cttaccgcgc
    15781 atctcaacca gacgcaccgc atcagcgaaa tcaatcacgc cagcgcaaac cagcgcggag
    15841 tattccccca ggctgtgacc ggccatcatt gccggtgctt taccgccctg ctgctgccat
    15901 acgcgataca gcgcaacaga tgcagtcaac agcgctggct gggtttgcca ggttttattc
    15961 agttcttcag ctggcccctg ctgggtcagc gcccacaggt cgtagcccag cgccgcagaa
    16021 gcttcagcaa acgtttcttc gacaattgga tagctcgccg ccatatcagc cagcattcca
    16081 acggtttgag aaccctgtcc agggaacaca aatgcaaatt gcgtcatgtt ttaatcctta
    16141 tcctagaaac gaaccagcgc ggagccccag gtgaatccac cgccaaaggc ttcaagcaga
    16201 accaactgcc ccggcttaat gcgcccgtcg cgtacagctt catccagcgc gcacgggaca
    16261 gaggccgcag aggtattacc gtggcgatcc agcgtcacca cgacattgtc catagacata
    16321 ccgagttttt tcgccgttgc actgataata cgcaggttag cctgatgcgg aaccagccag
    16381 tccagttgag aacggtcaag attatttgcc gccagcgtct catcaacgat gtgcgccagt
    16441 tccgttaccg caaccttgaa gacttcgttg cccgccatcg tcagattaat tgaattctct
    16501 ggattcacac ggtcagcatt aggcagcgtc agcaactcac catagctacc gtcggcatgc
    16561 agatgggtgg agatgattcc cggctcttca gaggcagcca gcaccgcagc gcccgcgcca
    16621 tcgccaaaaa taataatagt cccacgatcg gttggatcgc aggtgcgcgc cagtacatcg
    16681 gaaccgacga ccagagcata cttcaccgcc ccagatttca cgtattgatc ggctacgctt
    16741 aatgcatagg tgaaacctgc gcaggctgct gcaacgtcaa atgccgggca acctttaatg
    16801 cccagcatgc tttgaatctg acaagctgcg ctcgggaaag cgtgcgtagc agaagtcgtt
    16861 gccacaacga tcaggccaat ctggtctttc tcaatgcccg ccatctcaat tgcgcgtgtc
    16921 gccgcttcaa agcccatggt tgaaacggtt tcgtttggcg cggcaatgtg gcgttcgcgg
    16981 ataccggtac gagtgacaat ccactcgtca gaggtgtcca ccattttttc caaatcggcg
    17041 tttgtccgca cttgttcggg cagatagctg ccagtaccaa taatcttcgt atacatgtac
    17101 gctcagtcac ttttcggtta tataccgtca cttgcaaact gcgagttcgc tggcagcgtc
    17161 ctgctaccgc agagttccgc ttttgccacc gtccagcagc tcaaaaccag ctgggtatac
    17221 agattccagg cgagcggcaa ttcgctgagg aacttgtcgc tgcaccgcct gcactgcctg
    17281 ttcaatcgcg accgcaaaag ctcgctgatt ggctgcacca tgacttttta tcaccgtgcc
    17341 gcgcaatcct aacagacagg cgccgttata ctggtcgggg ttgaggtgac tgaatcgcct
    17401 cgtcaggctc ttttgtagcc aacgctttaa taacagtagc caccacgacc gttttttccc
    17461 ttcaccctga gatttcagca gagaaaggaa catcctgaca acaccttcca tcgtctttaa
    17521 cgtgacattt cctgtaaagc cgtcacaaac cagcacatct gtcttgccag ttaacaactc
    17581 attggcttca agatagccga tataattgat agaagggatt gttttaagca ccgctgaggc
    17641 atcccgaata ctgtcgagac cctttacttc ttcttcacca atattgagca acgccacgcg
    17701 aggattggga atttccacca cctcttcagc caggactgag cccataatgg caaattgcac
    17761 caacattgtg ctgtcacaat cgacgttggc ccctaaatcg aggaccaccg ttttgccctt
    17821 ttgctgatgt ggtaataccg tcaccagcgc cggacgctca atcccctcca ggggcttgag
    17881 taataatttt gccagcccca tcagtgcccc ggtattaccg gcactgacac aggcttgcgc
    17941 tcgaccttct ttcaccagct ccagggccat acgcattgaa ctcccacgac tggcgcggat
    18001 agcttgcgaa ggccgggcat cactggcgat aactgactgc gcaggaataa tctgcagacg
    18061 cgaacgttgt tcaaagtcag ctttagcaag taatggcgtg atggcgtcgg gattgccgac
    18121 taaaagaaga gtgagttgcg aattagaatt cagtgcctgc aatgctgcag gcactgtcac
    18181 ggaagggcca aaatcccctc ccatgacatc taacgccagg gttagacgtg tcaaggtatc
    18241 gtcgccgccc ggtttggtct ttccccagtt gcctggggaa aatcctcact aagcttcatc
    18301 acgcagacgc gtgattactt agcgatgacc ttgcggccgc ggtagtaacc gtcggcagtg
    18361 atgtggtgac gcaggtgttt ttcaccagaa gttttgtcta cagacaggct ggtgactgcg
    18421 gtcagcgcgt catgggaacg acgcatgcca cgtttggaac gggttggttt attctgttgt
    18481 acggccatgg accttactcc tcaattacgg tttatccccg atccaacggg gagcaccaat
    18541 tacttacgct ttaagctggc taatacggca aatgggtttg gcttttgcgc ttcttcaggc
    18601 agttcaccaa agaccatgtc cgcttcggac acttcacagt gttcagaatc atgcaccgga
    18661 actaccggca aggcgaggat gatttcatct tcaaccattg caagcagatc gatttcaccg
    18721 aattcgttaa cctcaatcgg ttcatacgct tccggcagtg cttcagcctg ttcgtctgaa
    18781 cgcacaggac taaaacaata cgttgtgtag acctgatgag taaacggctt cccgcaacgc
    18841 tgacactcga gcgttaccgt caccttcgca tcgccgttta acactgcgag acgttggtta
    18901 tcgatagcga acgacatgga gcattccaca tcactgtcca cactgactac ggattcggcg
    18961 acgcgctcaa cctgatcagg ggtatagata ccctggtaat caaggcgttt ttgagccgta
    19021 cgaaccggat cgagagtcag gggtaatttt accttttgca tagggcgcgc atattaactt
    19081 tgtaacgtca tagagtcaaa gaaaaaggca gcctgtggtt gccttttgcc aataattcgc
    19141 acacattgcg ggttactgct ttattttcgt tcagtgctga taacgaacat caacctgcaa
    19201 aacgaagcgc atagtttaaa atggctatca tcaatacgct atatctgatg gaaaaaaata
    19261 tgcctaaact tattttagcc tccacctcgc catggcgtcg cgctcttctg gaaaaactgc
    19321 aaatctcttt tgaatgtgca gcaccagagg tcgacgaaac cccacgcagc gacgaatcac
    19381 cgcgacagtt ggtgcttcga ctggcacaag aaaaagcgca atctctggcg tcacgttatc
    19441 cggatcattt aattattggt tcagaccagg tatgtgttct tgatggcgaa atcaccggta
    19501 aaccgttaac ggaagaaaat gcccgtctgc aattacgcaa agccagcggc aatatcgtca
    19561 ccttctatac tggactggcg ttgtttaatt cggcgaatgg gcatctacaa acagaagtgg
    19621 agccttttga cgtccatttc cgtcacctga gcgaggcgga gattgataat tacgtgcgta
    19681 aggagcatcc cctgcactgc gcgggtagct ttaagagtga aggatttggc attacgctgt
    19741 ttgagcgttt agagggacgt gaccctaaca cgctggttgg tttaccgcta atcgcgctgt
    19801 gtcagatgtt acgtcgggaa gggaaaaacc cgctgatggg atagttgttt gcgccggatg
    19861 cggttacgtc gcatccggca atcggtgcag gcggcctgat gcggcgtaaa cgccttatca
    19921 ggccaaccgc ccattatcag gcttatatgc ttgtattagc gcgcgttacg cagcttttgc
    19981 aaacaacgct tcaaaccttc atccatcggc gcttcgatac gcatcacctc accggtcccc
    20041 ggatgggtaa acttcaacgc tgcggcgtgc aggaacagac gatttaatcc cgtgcctgct
    20101 tcagtgagct gtctgtcaaa ttcacggtca ccgtagcgat cgtcaaaggc aatcggatga
    20161 cccgcatact gtgtatgcac acggatctga tgagtacgtc cggttaccgg gctacaacgc
    20221 accagggtgg caaatgcata gcgttcttcc actttaaagc gtgtttccga cggtttgcct
    20281 tcctgactga cacgcacgat acgttcgccg ctttgcagaa tatttttcaa taacggcgct
    20341 tgaacgctct tcacatgcga ctgccactga ccgcgcacca gcgccaggta atctttttgc
    20401 attccttttt cacgtaattg ctcatgcaga gaacgcaacg ccgagcgttt tttcgctacc
    20461 agcaacacac ctgaagtgtc ccggtcgaga cgatgaacca gttcaaggaa ccgcgcttcc
    20521 gggcgcaacg cccgcaaacc ttcgataacg ccgaagctta aaccactgcc gccatgtacc
    20581 gccgtaccgg aaggtttatt cagcaccagg atatgatcat cttcatataa gatgacgtcc
    20641 gccagcgccg ccaccttttg cagatgtggc gaaaccgcct cttcttcccg ctcagcaacg
    20701 cgaaccggtg ggatacgcac ctcatcaccc gcttcgagtt tatattcagg cttaatacgt
    20761 tttttgttca cccgcacttc acctttacgc aaaatacggt aaatcatact ttttggtacg
    20821 cctttcaatt gggtacgcaa aaagttatcg atacgttgcc ccgcttcgtc agcggtgata
    20881 gcaacaattt ttacggatgg agtctctgtt ttcatggtgg gcgattctaa ttagccaaca
    20941 ggattcgcgc cactcatttt tctatgctta tatttagttt tacaccttat tacttcacta
    21001 cgtgatcact ttattgatgg ttattaaacc aatcaccagc aagaagtgaa aaaactgtga
    21061 gtaagcgggt gataaatggt aaaagtcatc ttgctataac aaggcttgca gtggaataat
    21121 gaggccgttt ccgtgtccat ccttgttaaa acaagaaatt ttacggaata acccattttg
    21181 cccgaccgat catccacgca gcaatggcgt aagacgtatt gatctttcag gcagttagcg
    21241 ggctgcgggt tgcagtcctt accggtagat ggaaatattt ctggagagta atacccagtc
    21301 tgtttctttg ataattgcgc tgtttttccg catgaaaaac gggcaaccga cactctgcgc
    21361 ctctttgagc tgacgataac cgtgaggttg gcgacgcgac tagacacgag gccatcggtt
    21421 cacacccgga aaggcgttac tttgcccgca gcttagtcgt caatgtaaga ataatgagta
    21481 agttacgatg aaaagaatgt taatcaacgc aactcagcag gaagagttgc gcgttgccct
    21541 tgtagatggg cagcgtctgt atgacctgga tatcgaaagt ccagggcacg agcagaaaaa
    21601 ggcaaacatc tacaaaggta aaatcacccg cattgaaccg agtctggaag ctgcttttgt
    21661 tgattacggc gctgaacgtc acggtttcct cccactaaaa gaaattgccc gcgaatattt
    21721 ccctgctaac tacagtgctc atggtcgtcc caacattaaa gatgtgttgc gtgaaggtca
    21781 ggaagtcatt gttcagatcg ataaagaaga gcgcggcaac aaaggcgcgg cattaaccac
    21841 ctttatcagt ctggcgggta gctatctggt tctgatgccg aacaacccgc gcgcgggtgg
    21901 catttctcgc cgtatcgaag gcgacgaccg taccgaatta aaagaagcac tggcaagcct
    21961 tgaactgccg gaaggcatgg ggcttatcgt gcgcaccgct ggcgtcggca aatctgctga
    22021 ggcgctgcaa tgggatttaa gcttccgtct gaaacactgg gaagccatca aaaaagccgc
    22081 tgaaagccgc ccggccccgt tcctgattca tcaggagagc aacgtaatcg ttcgcgcatt
    22141 ccgcgattac ttacgtcagg acatcggcga aatccttatc gataacccga aagtgctcga
    22201 actggcacgt cagcatatcg ctgcattagg tcgcccggat ttcagcagca aaatcaaact
    22261 gtacaccggc gagatcccgc tgttcagcca ctaccagatc gagtcgcaga tcgagtccgc
    22321 cttccagcgt gaagtccgtc tgccatccgg tggttccatt gttatcgaca gcaccgaagc
    22381 gttaacggcc atcgacatca actccgcacg cgcgacccgc ggcggcgata tcgaagaaac
    22441 cgcgtttaac accaacctcg aagctgccga tgagattgct cgtcagctgc gcctgcgtga
    22501 cctcggcggc ctgattgtta tcgacttcat cgacatgacg cctgtacgcc accagcgtgc
    22561 ggtagaaaac cgtctgcgtg aagcggtgcg tcaggaccgt gcgcgtattc aaatcagcca
    22621 tatttctcgc tttggcctgc tggaaatgtc ccgtcagcgc cttagcccat cactgggtga
    22681 atccagtcat cacgtttgtc cgcgttgttc tggtactggc accgtgcgtg acaacgaatc
    22741 gctgtcgctc tctattctgc gtctgatcga agaagaagcg ctgaaagaga acacccagga
    22801 agttcacgcc attgttcctg tgccaatcgc ttcttacctg ctgaatgaaa aacgttctgc
    22861 ggtgaatgcc attgaaacgc gtcaggacgg tgttcgctgc gtgattgtgc caaacgatca
    22921 gatggaaacc ccgcactacc acgtgttgcg cgtgcgcaaa ggggaagaaa cgccgacctt
    22981 aagctacatg ctgccgaagc tgcatgaaga agcgatggcg ctgccgtctg aagaagagtt
    23041 cgctgaacgt aagcgtccgg aacaacctgc gctggcaacc tttgccatgc cggatgtgcc
    23101 gccagcgcca acgccagctg aacctgccgc gcctgttgta gccccagcac cgaaagctgc
    23161 accggcaaca ccagcaactc ctgcacaacc tgggctgttg agccgcttct tcggcgcact
    23221 gaaagcgctg ttcagcggta gtgaagaaac caaaccgacc gagcaaccag caccgaaagc
    23281 agaagcgaaa ccggaacgtc aacaggatcg tcgcaagcct cgtcagaaca accgccgtga
    23341 ccgtaatgag cgccgcgaca cccgtagcga acgtactgaa ggcagcgata atcgcgaaga
    23401 aaaccgtcgt aatcgtcgcc aggcacagca gcagactgcc gagacgcgtg agagccgtca
    23461 gcaagctgag gtaacggaaa aagcacgtac caccgacgag cagcaagcgc cgcgtcgtga
    23521 acgtagccgc cgccgtaacg atgataaacg tcaggcgcaa caagaagcga aggcgctgaa
    23581 tgttgaagag caatctgttc aggaaaccga acaggaagaa cgtgtacgtc cggttcagcc
    23641 gcgtcgtaaa cagcgtcagc tcaatcagaa agtgcgttac gagcaaagcg tagccgaaga
    23701 agcggtagtc gcaccggtgg ttgaagaaac tgccgctgcc gaaccaattg ttcaggaagc
    23761 tccagctcca cgcacagaac tggtgaaagt cccgctgcca gtcgtagcgc aaaccgcacc
    23821 agaacagcaa gaagagaaca atgctgataa ccgtgacaac ggtggcatgc cgcgtcgttc
    23881 tcgccgctcg cctcgtcacc tgcgcgtgag tggtcagcgt cgtcgtcgct atcgtgacga
    23941 gcgttatcca acccagtcgc caatgccgtt gaccgtagcg tgcgcgtctc cggaactggc
    24001 ctctggcaaa gtctggatcc gctatccaat tgtacgtccg caagatgtac aagttgaaga
    24061 gcagcgcgaa caggaagaag tacaagtgca gccgatggtg actgaggtcc ctgtcgccgc
    24121 cgctgtcgaa cctgttgtta gcgcgccagt tgttgaagaa atggctgaag tcgtagaagc
    24181 ccccgtaccg gttgccgaac cgcaaccgga agtggttgaa acgacgcatc ctgaagtgat
    24241 tgccgccgcg gtaactgaac agccgcaggt gattaccgag tctgatgttg ccgtagccca
    24301 ggaagtcgca gaacacgcag aaccggtggt tgaaccgcag gaagagacgg cagatattga
    24361 agaagttgcc gaaactgctg aggttgtggt tgctgaacct gaagttgttg ctcaacctgc
    24421 cgcgccagtc gtcgctgaag tcgcagcaga agttgaaacg gtagccgcgg ttgaaccaga
    24481 gatcaccgtt gagcataacc acgctaccgc gccaatgacg cgcgctccgg caccggaata
    24541 tgttccggag gcaccgcgtc acagtgactg gcagcgccct acttttgcct tcgaaggtaa
    24601 aggtgccgca ggtggtcata cggcaacgca tcatgcctct gccgctcctg cgcgtccgca
    24661 acctgttgag taataattag ctcaaagtaa tcaagccctg gtaactgcca gggctttttt
    24721 atttcatctt tgaatcaaca tattaaaatt catcaaatgg catccttgct aaccaacaat
    24781 gcaaaatagg caaaaaaaat gcccgccccg gcgatcccag agcaggcaga caaaacatac
    24841 ccagtttcat gatatgtttt aaagcgagct tatttgttga gctggaagag cgacaatccc
    24901 tgcatatcgg taaatgcttt atacgatgcc tgcaatgccg tttgctgcat gatgtaagat
    24961 gaaatagttg cattccagtc cacatcaacc agatcgctca tctgctgcgt ttgccctaaa
    25021 gcgcgatcgc tacctaagga atccagcgac tccagttcgt tcagctgcgt gcctaattcc
    25081 gcgcgaacag tcagcacatt gttcagcgag tttttcagtc cgcggttggt tttatctaac
    25141 gccgcagcgg cggtttcttt atccgcttcg ctatccgcga ccggcgtttt cagggctgcg
    25201 atggcactat ccagcatggc aaaaagattg gtttcagaag cgctaccgtc tggttccgct
    25261 accgcgttgc tggtaatact gtcgaaaatt ttgtcacccg tgtgccctat caccatcgaa
    25321 cgcgaagcat cgacctgttg tttaatactt tctgctccac cgacgtattt ccctttctct
    25381 tcgctaaacg gcgcagtctc tgttttataa ccggcaaaaa tgtagcgccc gttaccgtca
    25441 gtggtgttcg ccagattcag caactggtca cgaagcccct gaatatccgt agccagcgag
    25501 gcccggtcat cgtcactcaa ggtgccattg ctggcgtaga caattttttc ctgagcattc
    25561 tggatagcag tggtgacctg gctaagtaca ctctcttcca gtgacacttt ttgagtggcg
    25621 aaagtacgtg ccagcgtgta ctggctgttt tgcgcctgtg cctgggagag aactacggct
    25681 tgtgatgctg caatgggatc gtcagaaggg ttaacgactc gcttacccgt cgacatctgc
    25741 tcgccgtact tcatccattc tgcctgagaa ttggtgatgc cgcgcatgtt ttgctggtac
    25801 atcatctggg tactgaaacg cattttatct ccccttagcg aatgttaatc agcgcatcaa
    25861 aaatcgcgtt tgccgtctgc agaacctgcg cattcgccag gtaatactgc tgaaaacgtt
    25921 gcagatttcc gtactcctca tcgagattga caccggaaat cgactgctgc tgattggaaa
    25981 gctgcgtcac cacattacct tgcgtggtgc tactggtttt caacgtcgcg gttttattac
    26041 cgatatcact cactaacgag gcataagcgt cgttaaagga tttcgcaccg cccaccgttt
    26101 tactgttgct ttgcagatcc agcagggcct gaccgttgcg gttgtcgcta tcacccgcat
    26161 cttcttcgct cgccatcgcg attttcgctt cgtcggtgat taatacatcc atgttgacga
    26221 tggcgtcact tactggtttc agcgtgaagc tgtcgttaac ggcaggcgtt cctgtaaacg
    26281 tcaactccag accatcaaat gccactttac cgttggcatc cggcgtcacc gtaaaagtgg
    26341 tattgctggc aaggcgggtg acctgccact gattattatc gaacgagatt ttgtaatctg
    26401 tcgccagtac cgcggaggca tcagttaccg tggcaccgat cgcaacgtca cctttgtttt
    26461 tcgtgttttg cagaaccgcg ggcttaccga tagcaaagaa atcttcacca gcatcgccgt
    26521 tggcatcaaa tccggctttg tgttggctgt tgaaagcctc ggcaaatgcc agcgccagtt
    26581 gtccaagcgt attacgcgtc tggtccagat cctgagaacg gaatgtcaga atgccgccca
    26641 gcgacccggt attcagtaat ttctccggga tctcaatatt gcctgccgtc ccatcaacgt
    26701 aagcgacagt cgtacgagaa gggtcggcgc tggaaggaac tgccgccagt tgccgcgccg
    26761 tacttccctg aaccagtgag taaccattgg ccatcgtgat gttataagta ccgccatcct
    26821 gaacgctgac ttctacgcca acaatctggt ttaattcgct caccagttga tcgcgttgat
    26881 ccagcagatt gttaggtgac gcccctgccc ccacgcctgt caggcgcgag atttgatcgt
    26941 tcaggctggc aatttgttta gcgtaattgt tgatctgatc aacgctggca cctatcgcga
    27001 tattgacctg tttgtcctgg tcgcgcagat attgatcggt ggttttaaac tgattcacca
    27061 acccttctga tttcccaatc agcgcctggc gcgctgccgg gtcttccgcg ttactcacca
    27121 gcgtttgcag gctggtgaag aaatcctgca tctgtgttgc cagcgaagag gtactggtgg
    27181 agagcatatt gtcgattttc gacatctgct catagcgggc ggtcaggccg ctactttgcg
    27241 tctgcgcagc acgtaactgg ttggtaataa acgcatcata ctcacgctgc acaccagaaa
    27301 cgtagacgcc attgccaacc cagccgccag cgcccaacgt gctattggcc tgcgccataa
    27361 tagtggtttg gcgggtatat ccggcaacgt tatagctgga gatattatta cttgccgtat
    27421 ttaacgccgc ctgggccgcg ttcagtccgc tcatggcgtt attgatcaag ctggacatgg
    27481 aggttcctta taagccttca attacagagt attatcggca gcgacccgcc ggacttgagt
    27541 tattcagaac agattatcaa tgttcatgct gtaggttttg ctcaccttgt cgcttatcga
    27601 tttcatctgc tgaatcatgt tggtgagttt gcgggcatag tgaggatcgg tggcatagcc
    27661 cgcgtcctgt agggcctgcg ccccctgttc cgcactcgcg gcggtcgtca cggcggcgta
    27721 gcgcgggtta cgcgttaaca gcccaacgta atccgacaag gcttccagat acgagctgta
    27781 gacgcgaaac ttcgctttta ctttcttcgc ttcgccgttt tcatattcag tcgtggtgat
    27841 ttcagtaact ggccctttcc agttgccaga ggctttgaca ccaaacaggt tatagctcgg
    27901 ctcgccgttt tcgcggcgga tttgccgttg cccccaacca gattccagtg ccgcctgagc
    27961 gaggatcaaa tgatgtggca caccgctttg ctggcttgcc agttgggcgg gcagcgagag
    28021 ttgcgcgagg aatgctttac tgtcacccgg cagcgaatca tcgtagttac gtggcacggc
    28081 cttttgcacc agctgcgaaa gcgcctgatt ttgataacgc accacggttt cgagcgggaa
    28141 tttcatcggt gctgctggcg tggactcctc tggcaatggt tgttctggcg tcatctgttt
    28201 aaccatcatc tctgcaagcc ccagaccttt gcccgccgtc atctgttggg caatctgctg
    28261 gtcatacata ctggtataca gacgagtgtg ctcgctgctg aacaggccat cttttggtaa
    28321 agcgtcgcgc atgcttttca acatcatctg cacgaacatc ccttccacct ggcgggccac
    28381 cggacggata tttgccgccg gatcttcgcc cgctttcgcc tttagttcgt tgagtgattg
    28441 cgcatcccag gccgcacttg ccagtagttt gctgtcgctg atcatcagat gatttccagt
    28501 tttgcccgca gacatcccgc actttgcatt gattgcaaaa tagacatcag atccatcggc
    28561 gtagcgccca gcgcattgag cgcgcgcacc acgttattga ggctggcgct ggaacgtacg
    28621 ctttgcagcg aaccgccgct ctggcgtaaa tcgatctgcg tttgtggagt aaccacagtc
    28681 tgtccaccac caaacggtgt atctggctgg ctgacattgg cctgacgatt gactgttact
    28741 gagagatttc cctgcgctac cgcgcagctg tcgagggtca cttcgcgatt catcaccacc
    28801 gaaccggtgc gcgagttaat cactacttta gcgtcctgcg gggtgacatt aacctgcata
    28861 ttctggatat ctgcaaggaa acggacctgg gaactgttgc cacttggtac gcgcacctga
    28921 atagtccggg catctaacgc ggtggcgctg ccatatccac gcacgcggtt gatggtgtca
    28981 gcgatttgct gcgccatgct gaaatcttcg tcgttaagtt gcaaattaag ggtattcccg
    29041 acgccaaact ggctgggcaa ttcacgttca ataaccgcac cattggtgat ccgtccaccg
    29101 ttcagttggt tcacctgaac actgctaccg ccagcggagg ctcctgcgcc gccaaccaga
    29161 atattgccct gcgccagcgc atacacctga ctgtcaacgc ccttaagcgg cgtcatcaac
    29221 aacgtacctc cacgcaagct tttggcattt cccatggaag aaaccaccac atcgatggtt
    29281 tgcccctgac gtccaaacgg aggaagtgac gctgtcacca ttaccgcagc gacgtttttt
    29341 agctgcatat tggtgcccgt cggaacggta attcccagct gtgagagcat gttattaagc
    29401 gtttgtgtgg taaacggcgt ctgggttgtc tggtcaccgg tgccatccag ccccaccacc
    29461 agaccatagc caatcagtga gttttgcctt accccctgaa cactggtgag atcgcgaata
    29521 cgctcagcct gagccgccgt cgtaaccagt agaagaatta atgcagagag aaatttaatc
    29581 accacagcct cacttacatt ggcgacaggt taaggaagaa acgctgcaac cagcccatat
    29641 tttgcgcttc gttaatgtag ccattgccta cgtattcaat gcgcgcatcc gccacctgag
    29701 tagacggtac ggtattgctg ccgctgatag tgcgtggatt aaccacgcca gagaagcgaa
    29761 taaattcggt accctgatta atggcaatct gtttttcacc caccacatgc aggttgccgt
    29821 tgaccagtac ctggtcaacc gtcaccgtca acgtgccgct aaaggtattg ctggcattgg
    29881 ccccgccctt tccgttgaac gtgttaccac cggaggcttc gacatcggca cgagcgttac
    29941 caaacagccc ctgcaaatag cgcggcacag tatcaaagcc aaaattagtt ttaccgtcac
    30001 ggctggcatt cgcagaggag cttttgctgg cgctgacgtt ctcctgcaac acgatggtca
    30061 gcgtatcgcc aatattgcgt ggtcgacgat cttcaaacag cggttgatag ccatagttaa
    30121 tcggctgagc agactggaaa atagaaccgt tggcgacggg cgtcggaccg ggaaccggtt
    30181 gtgcactggt cgccccctgc accagcggcg tggagggtat ccaggcgcag ccggttagtg
    30241 aaagcaccaa caagctggaa atggcataag tatgcgcagc gtttttttgc atggctatca
    30301 tcttcaaaaa tcagtaaacc ggtgaacctg ccaccggtta agccttagag ttgcgtcagt
    30361 ttttgcagca tctgatcggt ggtggacacc gctttactgt tgatttcgta agcgcgttgc
    30421 acctgaatca tattgaccag ttcttccgcc acgttgacgt tagacgtttc aacataccct
    30481 tgatacagca gtcccgcgcc gttcaggccc ggcgtgcttt cgttcggtgc accagaggat
    30541 tgcgtttcgg tgtagaggtt ttcgccaatg ctctccagcc cggtgtcatt cataaaggtg
    30601 gtgagattga gctgcccaac ctgaaccgga gctgcctggc cttgttgggt tacgctgacc
    30661 acgccatcac gaccgatggt gatacttaac gcattcgccg gaatggtgat cgctggctgc
    30721 acctgaaaac caccagccgt caccagctgc ccgttctgat ccacctggaa agagccgtca
    30781 cgggtatagg ctgatgaacc atctggcaac atcacctgga aaaagccctg ccctttaatc
    30841 gcgacatctt tgctgttgtt ggtctgcgac aggtttccct ggctgtgtaa gcgttcagtg
    30901 gcgaccgggc gtacccccgt gccgatttgt aatccggagg gtaaggtggt ttgttcggaa
    30961 gactgtgccc ccggctggcg aatggtttga taaagcagat cttcaaacac cgcgcgctga
    31021 cgcttaaaac cgttagtact gacgtttgcc aggttgttgg caatgacgtc catattggtt
    31081 tgctgggcgt caaggcccgt tttggcgatc cataatgaac tgatcataaa atgtcctgta
    31141 gtggattagt gacaagcaga gcagaagcac gcgacagtgt gaatttcccc tcaccctaac
    31201 cctctcccca aaggggcgag ggaatagtcc gcgcgctttt attgcaatgt catcaatcgt
    31261 tcaattaact catcgacagc agttggttgg cacggcctgc gttatcatcg acgctgctga
    31321 tcaccttcat ctgcatttca aaacgccgcg cgctggcaat catgtcgctc attgccgcaa
    31381 cggcattgac gttactgcct tccagaaccc ccgacatcac acgcaaggtt ggatctgcct
    31441 gcagtatcgg cccacgcgtg gcctggcttt ctgcgcttaa acgaaaaatg ccgtcgtcac
    31501 cgcgttgcac ttcgctgccc gtggctttca ccagtttcag acgccccact ggcgcaaccg
    31561 tatttgccgg atcgcccgga ttgagcgccg agattgtgcc atcggcagca atagtgattt
    31621 ccgccccttc cggcacagca attggcccag cctcgcctat caccggatgc ccctgaattg
    31681 tcagttgccc ggtgggatca acctgaatgc tgccattacg cgtataccct tcgctgccgt
    31741 cagcggactg cacggccagc cagccatcct gctgcaatgc aacgtccagc gggcgcgagg
    31801 tgtaatccat cttgccgggc gtcatgtctg cacccggtgt tgacgccgtt accagcgtgc
    31861 gcgtgggcag tgaaagccct tccaccggca ctgcacgcaa ggcgtttaac tgcgcgcgaa
    31921 aaccgggcgt agaggcattg gccagattac tggcggttac cgcctgttga ttcagcgtct
    31981 ggctggctgc tcccatcgcg gtataaattg cgtgatccat tgagctatcc cgtcagcgat
    32041 tagcgtaagt taaccagcgt gttgaggatc tggtcctggg ttttgatggt ctgggcgtta
    32101 gactgatagt tacgctgggc aacgatcata ttgaccagtt ctttactgag atcgacgttg
    32161 gacgcttcca acgcaccgtt ggtcagggtg ccaaagttgc ccgtcccggc tgtccccaac
    32221 agcgccacgc cagaagattg cgtcgcagac cagacgttgt cgccttcgga tgccagacct
    32281 tcgttgttgg caaagttcgc cagtacaatc tgccccagca gttgtgtttg ttcgttggaa
    32341 tagttgccga caaccgtacc gtcatcattg atttgataac tcaccagatc gcccggtttg
    32401 tagccattct gggtggttgc cacaatattg ttagcgccgg tattttgctg catggagttg
    32461 aggaagctca ggctaaacgt ggcgggatct gcgccgttaa ttgcgccagt ggtaatattc
    32521 tctgttggat ttgaggtcag aacgccattg gcattaaaca ccagcttcat tgcaggctcg
    32581 gctgtacctg ttggatcact gctatcctgg gtgtagacct gccagttatt atccccggtc
    32641 ttcacaaagt agacgctcat gtcatgagca ttaccctgac tgtcgaaaac agtcaccgaa
    32701 ccttttttgt tatagctatc cgcattgctg gcgctgaatg gcgtaacagt aggaagcgga
    32761 tcactggaat tcaggttgat ctgcatcgat gccgtggtgg tagttttcgc tgccatcagg
    32821 gtattcggga tcgaaatatt ggtcggattc gccccttgct gaatagtcgg cggcgtaccg
    32881 gttgcctggt aacccgtcag ctgtaaacct tgcatattca ccaggttacg gttttcatcc
    32941 agcttaaatt gtccgttacg gctgtagaac accgaaccgt tgctgtctac cagacggaaa
    33001 aaaccgttct ggctgatagc aacgtccaga cctcgcccgg tgttggtggt cgtgccatcg
    33061 gtaaagtcct gagtgatacc ggcaactttt acccccagtc ccactttcga accggcaaac
    33121 atatcggcaa aagaggccgt gcctgattta aagccgtagg tggcggagtt ggcgatattg
    33181 ttgccaataa catcgaggtt ggtggcggca gcgtttaatc cgctaaccgc ttgagaaaag
    33241 gccatgactg actcctgaaa tgtgaaggct taaattatct gccgtacttc gtcgagggtg
    33301 gtggtgccgt aagtgccgag atccagcgta ttaccgctgt tgccgcggat cacaccctgc
    33361 accagagcaa actgcagcgg ctgggcaacc agttgtgtac caccgttact ggcgctaatc
    33421 gctacattgt aagaaccgtt cggcgcagtg ctgccatcag tcaacgtacc gtcccaggtg
    33481 aaactgtgaa ctccagcggt cagttcacca atatcaatgg tgcgcacaac cgcgccattt
    33541 ttatcggtga tggtggccgt aactttgtct gccgcctgtt gcagctcaac accaaacggc
    33601 gtggtcgtgg tcacagcccc ttcttcactg ccggttcccg caagaacagt tgtgccgggg
    33661 atcatcacgc cgtgaccgat caggttactg gcctgtaacg actggctgtt atcaatctgt
    33721 ccggaaatag atccgagtgt ggtattgagt ttttcaatcc cgctgaccgt gctgatttgt
    33781 gccaattgcg acgtcagctc gttgttttcc attggattgg tcgggtcctg gtttttcagc
    33841 tgcgccacca gcaaagtcag aaaactgctt tgtaaatctg cggcgttgct gcccgtgagc
    33901 gaactactgc tggtggtact gacgccggta tttgtcggat cggtggtggt taccgcaatg
    33961 gacatagctt tctcctttat tgaccgagcg taagggtttt cagcatcatg cttttcaccg
    34021 tgttgagcac ttcaacgttg gcctgatagc tgcgtgacgc cgacatggtg ttaaccatct
    34081 ccccgacgac atcaacgttc ggcattttta cgtagccctt tgcatctgcc agcggattac
    34141 ccggttcata aaccagtttg tccggggcct gactttctat aacatcggca acctttacgc
    34201 cgcctgtcgc agcacctggt gcagcgttaa cctggaatac cacctgtttt gcccgatatg
    34261 gctgtccatc aggaccggtc acgctatcag catttgccag attactggcc gccacgttca
    34321 ggcgctggga ctgggcagtt aacgccgacc cggcgatatc aaaaatattc agcagtgcca
    34381 tccgttaatt tccgctctgt aaaacgttca tcatgccttt gatttgcccg ctcaacgcgc
    34441 taaggctcat ctggtattgc aggctgttat cggcaaactg ggtgcgttcg cgatccatat
    34501 cgacggtatt accgtcaagc gaaggctggt ccggaatacg gtattgcagt tctgcggtag
    34561 gaggcgtcag cgcctgcgcc gggatgtgtt gcgttgaggt catcgtcagt gcaaccacac
    34621 tggttgcatc ccgtccacgt tgcatgactt ttttaagttc actggcaaaa tcgatatcgc
    34681 gcgcctgata accaggggta tcggcattgg cgatgtttgc tgccagcact tcctgacgct
    34741 gggcgcgcag attgagcgcc tcttgttgaa aacgtaaggc ggcgtcgagc ttatcgagca
    34801 tatctcctcc gcaggtatca aaattctgcc atcagcttaa atgcctttac gcgcgcgtta
    34861 tcggcggaat aaacgcaaaa tgggtcgcta tttatgccgt tgatggtcat tgcggacagg
    34921 tacaattcac gttgtagaaa tggctggggg cgaaaatgct gacaataaaa cgtagcgtgg
    34981 cgatcatcgc gatactgttc agtccgttaa gtacggcgag caatctcaca tcgcaattgc
    35041 acaacttttt tagcgcccaa ctcgcggggg taagtgatga ggttcgtgtt tctattcgta
    35101 cagcgcccaa tctactaccg ccatgcgagc agccattgct ttcgatgagc aataattccc
    35161 gcctgtgggg caatgtgaat gtgttggcac gctgcggtaa cgacaaacga tatttacagg
    35221 ttaatgtgca ggccacagga aattatgtgg ttgccgcgat gcccattgtg cggggaggaa
    35281 agctggaagc tggcaatgtc aaactaaaac gcggacggct ggataccctg ccaccgcgta
    35341 cggtgctgga tatcaatcaa cttgttgatg ccattagcct gcgcgatcta tcacccgatc
    35401 aacctatcca gttaacccag tttcgccagg catggcgggt aaaagcggga caacgcgtca
    35461 atgtgatcgc cagcggtgat gggtttagcg ccaacgcaga aggtcaggcg ctgaacaatg
    35521 cagccgtcgc acagaatgcg cgggtgcgca tggtatcggg acaggtagtc agcggcgttg
    35581 ttgatgcaga tgggaatatt cttataaacc tgtaagctgt taaagattac ccgtcccttg
    35641 ccgataaata agcaacacat gataaaagcg ccctcaatga ggaataaacc atgagtattg
    35701 atcgcacttc gcctctgaag cctgtaagca ccgttcaacc gcgcgaaacc actgacgcgc
    35761 cggtaacgaa cagccgggcg gcaaaaacaa ccgcctccac cagcaccagt gtgacgttaa
    35821 gcgacgcgca agcaaaactg atgcaacccg gcagcagtga tatcaatctt gaacgtgtcg
    35881 aagcgttaaa actggcgatt cgtaacggtg aactaaaaat ggacaccggc aaaattgccg
    35941 atgcgctgat caacgaagcg cagcaagact tgcagagtaa ctgatcgtat gacacgtctt
    36001 gcagagatcc tcgaccagat gtccgctgtg cttaacgatc tcaaaacggt aatggatcaa
    36061 gagcagcaac atctctctat ggggcagatc aacggcagcc agttgcaatg gattacagaa
    36121 caaaaaagct cactgctggc gacgctggat tacctcgaac agttacgcaa gaaagaaccc
    36181 aatacagcaa atagcgttga tattagtcaa cgctggcagg aaattactgt gaaaacgcag
    36241 caactacgcc aaatgaatca acataacggc tggttactgg aaggacagat tgagcgcaat
    36301 caacaggcgc tggaaatgtt gaaaccgcat caggaaccga cgctatatgg ggcgaacggt
    36361 cagacctcaa caacccatcg cggcggtaaa aagatttcga tctgaatatt atctccggcc
    36421 tgcactgcag gccggaatgc attgttacac catccggcgg gcaaattctt taactttgaa
    36481 gcccagtacc gccagtgcag cgaagtacgc ggcaatcccc gccagcacga ccgccattaa
    36541 acgcagtaag cgccagggca tggtacccaa tgaccactcc ggcatgatat gtaacatacc
    36601 taaaagcacg ccagacatca ccagtaccgc caccaccaga cgcaacagaa acgccatcca
    36661 gccgggttgc ggagtaaaga ttttctgctt acgcaactgc cagtaaagca gcgaagcatt
    36721 cagacacgcc gccagaccaa tagaaagtga cagcccggca tgtttcaacg gaccaataaa
    36781 cgccaggttc atcaattgcg ttaaaatcag cgtaacgatg gcaattttca ctggcgtttt
    36841 aatgtcctgg cgggaataaa agccaggagc caacactttc actacaatca ggccaatcaa
    36901 acccaccgag taggcaatta acgcccgctg ggtcatcagc gcatcaaacg cggtaaattt
    36961 accgtactgg aacagcgaaa cggttaacgg acctgaaaga atgcccaacg caaccgcact
    37021 cggcagcgcc aacaggaaac aaagacgcaa cccccagtcc atcaaacggt tgtattcatc
    37081 atgattgcca ctggcaaaac ttttcgacag cgacggcagc aaaatggtgc caagcgccac
    37141 gcccagcaca ccggacggaa actccattaa gcggtcggcg taatacatcc aagacaccga
    37201 accggaagca agaaacgagg caaaaatggt gttgatgatt aaggagatct ggctcacaga
    37261 gacgccaagg atcgccggtc ccatctgttt caccacgcgc attgctccgg catcgtggaa
    37321 gttaatgcgc ggcaggacca gcatgccgat cttcttcagg tgcggtagct gatacaccag
    37381 ctgcaggatg ccgccgaccg taacagccca cgccagcgcc agcaccggtg ggttaaagta
    37441 cggtgcggca aacagcgcga aaccaatcat gctgatgtta agcagtgttg gagcaaacgc
    37501 cggaatcgag aagcggttcc acgtattcag aatcgctccc accagcgacg ccagggagat
    37561 cagcaagata tagggaaagg taatctttag tagctggctg gtcagggcaa atttgtcagc
    37621 tgtgtcagcg aagcctggcg cggtcaccat gatcacccac ggtgcggcga gcatgccagc
    37681 gaccgtcaca accgccagcg cgagtgtcag caggccagaa acataagaga caaagacccg
    37741 cgtggcgtct tcaccctgct tacttttata ttccgccaga atcggtacaa atgcctggga
    37801 aaatgcccct tcggcaaaga tacggcgtaa caagttagga agtttaaaag cgacgaaaaa
    37861 ggcgtcggtt gccatccctg cgccaaagat tctggcgaca attgcgtctc gtgcgaagcc
    37921 aagcacacgc gaaaacatgg tcatcgagct gacggcggcc agcgatttta ataaattcat
    37981 cggtgttcta atccagaccc acgggcaaaa cgcctgcaag gcaggcgctg aaagaggcga
    38041 atagtctacc tggattatgg tgaattgcta ccgccagatg ttacagggtt tattcactca
    38101 tcgcatcgcg ccagatcttg tcaacgatac gttgcgccag cacggcctgt tcgccggcgg
    38161 tttgcggaac tgtctgattt tgcacacatt caatgaagtg ccgcgcacag ccgacaaatc
    38221 cacgttgttc aagagtgctc tgccaaccag gaatcggttt atgcactacg ccctgcccgc
    38281 gctcctcacg ccattcgcgc atatccgtaa tgtcgatgag cgcaccgtca gtcacggcct
    38341 gcacggtttc acgctgactt ccggcacggc gatgcataca ggtggtgatc tgcaaaggac
    38401 cggccgaaaa atggtgctcg gcaaacagca tttcgccagc gtcgttagtc agtagcgtac
    38461 cgccatccag agaggctttg ccgcccgaca accacagcgc ggtatccacc acatgcagat
    38521 aatcatccag caacgtgaaa taaagatcgt gtggcccgac actattgcta cgatgtttat
    38581 ccattcttag cgaagctgcg gtagcgagtt gcgtttttaa ctcaccgtag agtggtgcga
    38641 aacgacggtt aaaaccgacc atcaacgtca gttttttccg cgccgccagt tccaccagcc
    38701 gttcagcatc gcgcagattt tctgccagcg gtttatcgac acagacatgt acacccgcat
    38761 tgagtaacgt actgaccacg tcaaagtggc tggcggtgct ggaatgcaca aaaaccgcat
    38821 cgcaactggc ggcaaggctg gataacgaat cggcataagg aatgcgccag ctttcacaaa
    38881 ttggcagggc tttcgcgcgc gtaggcgacc aggctccttg taacatccag tcagacgctg
    38941 ccgccagcac cggtaaccac gctttttgcg caatgccgcc taatcccaca acgccgatac
    39001 gtaatttttt cactttaatc tcccagatgg gccagcaacg aatcaagacg ctgtttcagt
    39061 tctgccactt cgatttccag ggcttcgacg cgggcctgta aatcaccatc aaccgcgttc
    39121 gacatatccg ttaccgccgg ctgatcctct acctcaccac tgaaaagatg catgtagcgg
    39181 ctttcgcgtt tacccggttc gcgggccaga cgcaccacaa aaggaccatc ttcgcgattt
    39241 gccagttgtt ccagcgtcga ctccacttcc gccatatcgc tgaattcata cattcgcgcg
    39301 gcgcggctgc gcagttcacc tggcgtctgg gcaccacgca ataacaacgt ggtgattaac
    39361 gccacttccg ctgcgctcag tttcagatcg ccaaattctg aattacaaaa acgttgctca
    39421 tatttggtga cccgattacc aaaaccgctc actgtgcgta gataatgacg tttgaccaga
    39481 ttatccagct gttcctgcac ttcggattca ctcagattca tgaccggttc acggttcgtt
    39541 ttctgattac aggccgtgac tacaccattg actgagagcg ggtattgctc cggcgtggtc
    39601 acctgctttt ccagcaagca accaatcacg cgagcttcaa gggcagtaag ttgatatttc
    39661 atgagattct ccttagcggc cgggcgtcca gtctggggta gttaatgccg tcagtacgtg
    39721 atcgcgccat tgtccatcaa tcaacagata gtctttcgca tagccttctt tttcaaaacc
    39781 cagtcgcgcc agtaaatcac cgctgcgttt attgtgcggc atataattag ccataatgcg
    39841 atgaatatgt tgggtgcgct gcatataacg aatggctgcg gtcagggctt caaacatgag
    39901 tcctttgccc tgccattttt gcccaatcga ataaccgaga tagcaggcat gaaaagagcc
    39961 acgaacaaca ttggaaaaat tggcaacgcc aataatctct ttttcgtccg ggtcgaataa
    40021 gccaaagtag aaagctgaac cttgtttatg aaattcgtta atcatcccca gcctggcctg
    40081 ccagcctgat ggataacagt ggctttcgtc gcgcactggc tcccagggct tgaggaaatg
    40141 gcgattctct gcgtaataat ccgcaagacg ccaggcatca cgatcatgca ccagacgcac
    40201 gaccagtcgg tctgtggtta agcgcacttt tggcacgtta ctgcgatagc caaacattct
    40261 atacctactc cttcccgtaa cgtctttaat acgctgtaag tattactata cctgcgcaac
    40321 cagaccctgt gaaaagtcca aaatgccatt ttctgcatta ttcacatatt tgataaaaac
    40381 tctctatcaa agcgacgttt tgataaaaaa atattgtccc ctaagtgatg ggaaaagtct
    40441 tagcggcaat gcagaataga aagtgctcat cttttatttc ccgggagggg aaatgtcccg
    40501 cgtatcgcag gcgaggaacc tgggtaaata tttcctgctc atcgataata tgctggtcgt
    40561 gctggggttc tttgttgtct tcccgctgat ctctatccgc ttcgttgatc aaatgggctg
    40621 ggccgccgtc atggtcggta ttgctctcgg tctacgccag tttattcagc aaggtctggg
    40681 tattttcggc ggcgcaattg ccgaccgctt tggtgccaaa ccgatgattg ttaccggtat
    40741 gctgatgcgc gccgccggat tcgccacaat gggtatcgcc cacgaaccgt ggctattgtg
    40801 gttttcatgc ctgctctcgg gactcggcgg cacgttgttt gatccgccgc gttcggcgct
    40861 ggtggtgaaa ctaatccgtc cacaacaacg tggtcgtttt ttctcgctgt tgatgatgca
    40921 ggacagtgcc ggtgcggtca ttggcgcatt gttggggagc tggctgttgc aatacgactt
    40981 tcgcctggtc tgcgccacag gggccgtttt gtttgtgctg tgtgcggcgt tcaatgcgtg
    41041 gttgttaccg gcatggaaac tctccaccgt acgcacgccc gttcgcgaag gcatgacccg
    41101 cgtgatgcgt gacaagcgtt ttgtcaccta tgtcctgacg ctggcgggtt actacatgct
    41161 ggctgtacaa gtgatgctga tgctgccaat tatggtcaac gacgtggctg gcgcaccctc
    41221 tgccgttaaa tggatgtatg ccattgaagc gtgtctgtcg ttaacgttgc tctaccctat
    41281 cgcccgctgg agtgaaaagc attttcgtct ggaacaccgg ttgatggctg ggctgttgat
    41341 aatgtcatta agcatgatgc cggtgggcat ggtcagcggc ctgcaacaac ttttcaccct
    41401 gatttgtctg ttttatatcg ggtcgatcat tgccgagcct gcgcgtgaaa ccttaagtgc
    41461 ttcgctggcg gacgcaagag ctcgcggcag ctatatgggg tttagccgtc tgggtctggc
    41521 gattggcggc gctattggtt atatcggtgg tggctggctg tttgacctgg gcaaatcggc
    41581 gcaccagcca gagcttccgt ggatgatgct gggaattatt ggcatcttca ctttccttgc
    41641 gctgggttgg cagtttagtc agaaacgcgc cgcgcgtcgt ttgcttgaac gcgacgcctg
    41701 atttgatact cccccctgac tcctccatac tgaaagtgaa ctgacagcca aatggaggag
    41761 tcacgtgaag ctatacattt acgatcactg cccttactgc ctcaaagccc gcatgatttt
    41821 cggcctgaaa aatatccccg tcgaattaca tgttctgctc aacgacgacg cagaaacacc
    41881 cacccggatg gtcggtcaaa aacaggttcc cattctgcaa aaagatgaca gccgctatat
    41941 gccagaaagc atggacatcg ttcactatgt cgataaactc gacggcaaac cgttactgac
    42001 cggcaaacgt tcccctgcca ttgaagagtg gctgcgcaag gtcaatggct acgcaaacaa
    42061 actgctgttg ccgcgttttg ccaaatcggc attcgatgag ttttctactc ccgccgcgcg
    42121 caaatatttc gtcgacaaga aagaggccag cgcgggtaat tttgccgacc tgctggccca
    42181 ctctgacggt ctgattaaga atatcagcga tgatttacgt gcgctggaca aactgatcgt
    42241 caaaccgaac gccgtgaatg gcgaactttc ggaagatgat attcagctat ttccgctgct
    42301 gcgtaatctg acgctggtag ccggaatcaa ctggccaagc cgcgttgctg attaccgcga
    42361 taatatggcg aaacagacac aaatcaattt gttatcatca atggcgattt aatcctgacc
    42421 cgccgcggca gcctgtattc gcgggttgcc gccgttttca gttttttctg ctggcgagtt
    42481 tatcgctggt gacacatgct gcgttgtgtc aggatattga cgcagatttc atgtcgagga
    42541 ctaccatgaa caagttttta tttgccgctg cattgatcgt cagtggcctg ctcgttggct
    42601 gtaatcaact cacccaatac accatcaccg aacaagaaat taaccagtcg cttgcgaaac
    42661 ataataattt ctcaaaagat atcggtttac ccggcgtggc tgacgcccat attgttctga
    42721 caaacttgac cagccaaatt ggtcgcgaag agccgaataa ggttacccta accggagacg
    42781 ccaatctgga catgaactcc ctgttcggta gtcagaaagc gaccatgaaa ctgaagctga
    42841 aagcgctgcc cgtgtttgat aaagaaaaag gtgcgatctt cctgaaagag atggaagtgg
    42901 tcgatgcgac ggtacaaccg gaaaaaatgc aaacggtgat gcaaacgttg cttccctatt
    42961 tgaaccaggc attacgcaat tactttaacc agcaacctgc ttacgtcctg cgcgaagatg
    43021 gcagtcaggg cgaagcaatg gcgaaaaaac tggcgaaagg cattgaagtg aagccaggcg
    43081 aaattgtcat tccgtttacg gattaatgac gagggcgcat tcgcgccctt tatttttcgt
    43141 gcaaaggaaa acgtttccgc ttatcctttg tgtccggcaa aaacatccct tcagccggag
    43201 catagagatt aatgactgca ccatcccagg tattaaagat ccgccgccca gacgactggc
    43261 accttcacct ccgcgatggc gacatgttaa aaactgtcgt gccgtatacc agcgaaattt
    43321 atggacgggc tatcgtaatg cccaatctgg ctccgcccgt gaccacagtt gaggctgccg
    43381 tggcgtatcg ccaacgtatt cttgatgccg tacctgccgg gcacaatttc accccattga
    43441 tgacctgtta tttaacagat tcgctggatc ctaatgagct ggagcgcgga tttaacgaag
    43501 gcgtgttcac cgctgcaaaa ctttatccgg caaacgcaac cactaactcc agccacggcg
    43561 tgacgtcaat tgacgcaatc atgccggtac ttgagcgcat ggaaaaaatc ggtatgccgc
    43621 tactggtgca tggtgaagtg acacatgcag atatcgacat ttttgatcgt gaagcgcgct
    43681 ttatagaaag cgtgatggaa cctctgcgcc agcgcctgac tgcgctgaaa gtcgtttttg
    43741 agcacatcac caccaaagat gctgccgact atgtccgtga cggaaatgaa cggctggctg
    43801 ccaccatcac tccgcagcat ctgatgttta accgcaacca tatgctggtt ggtggcgtgc
    43861 gtccgcacct gtattgttta cccatcctca aacgcaatat tcaccaacag gcattgcgtg
    43921 aactggtcgc cagcggtttt aatcgagtat tcctcggtac ggattctgcg ccacatgcac
    43981 gtcatcgcaa agagagcagt tgcggctgcg cgggctgctt caacgcccca accgcgctgg
    44041 gcagttacgc taccgtcttt gaagagatga atgctctgca gcactttgaa gcattctgtt
    44101 ctgtaaacgg cccgcagttc tatggcttgc cggtcaacga cacattcatc gaactggtac
    44161 gtgaagagca acaggttgct gaaagcatcg cactgactga tgacaccctg gtgccattcc
    44221 tcgccgggga aacggtacgc tggtccgtta aacaataaaa attgtagctt cctgttgtca
    44281 ttaggttatt ttacctgtat aaataaccag tatattcaac agggggctat tatgcgaatt
    44341 gaagtcacca tagcgaaaac ttctccattg ccagctgggg ctattgacgc cctggctggc
    44401 gaactttccc gccgtattca gtatgcgttt cctgataatg aaggccacgt atcggtacgt
    44461 tatgccgcag cgaataattt atcggttatt ggcgcaacaa aagaagataa acagcgcatt
    44521 agcgaaattc tccaggaaac gtgggaaagc gccgatgact ggtttgtcag cgaataatat
    44581 gcagtgattt ttgtttttgc cgggtcgccc cggctttttt attttactta caaattttga
    44641 gtacaaaatt tctacacttc ctcaaaataa ttgcacaata tgctaaaaaa atatgaatga
    44701 catgctgttt tattgttcat ataaatctta aaagcagaaa tttatctgtt ccccccttta
    44761 aaaattcggt gaataccctt acttattggt atactgaagt tgcttcagat aaaaacgcat
    44821 tgaacctcga ataacgttgt ctagtaacac gaattagggg gccatgatgg aaaaaaataa
    44881 tgaagtcatt cagactcatc cgctcgtagg gtgggacatc agcaccgttg atagctatga
    44941 tgcgctgatg ttgcgtttgc actaccagac cccaaataag tccgagcagg aagggactga
    45001 agttggtcag acgctctggt taaccactga tgttgccaga caatttattt cgatattaga
    45061 agcaggaatc gccaaaattg aatccggtga tttcccggta aacgagtatc ggcgtcatta
    45121 acatgacatg accatccacc caaaaggcac ctcaccggtg cctttaccat ttctgggttg
    45181 tatatctgca ttcagttaat tactctgctt aaaccatgag agcagagaga aaaaaatgaa
    45241 atacgatctc atcattattg gcagcggttc cgtaggcgct gccgccgggt attatgcaac
    45301 ccgcgccggt ttaaacgtgc taatgaccga cgcccatatg ccaccgcatc aacacggcag
    45361 ccaccacggc gatacgcgat taattcgcca tgcttatggt gaaggcgaaa agtatgtccc
    45421 gttggtcctc cgcgcgcaaa cgctgtggga tgaactttcc cgccacaacg aagatgatcc
    45481 catttttgta cgctctggtg tcattaacct cggcccggct gactccgcat ttctcgccaa
    45541 cgtcgcccac agcgccgaac agtggcaact caacgttgaa aagctcgatg cgcaagggat
    45601 tatggcccgc tggccagaaa tacgcgtccc ggacaactac atcggcttat ttgagactga
    45661 ttccggtttt ttgcgcagcg aactggcgat taaaacctgg atccaactgg cgaaggaagc
    45721 gggctgtgcg caactgttca actgcccggt caccgcaatt catcatgacg atgatggcgt
    45781 aactattgaa acggttgacg gtgagtatca ggcgaaaaaa gcgattgtct gcgcgggaac
    45841 atgggtaaaa gacctgctcc cggagctgcc tgtccagccc gtacgtaaag tatttgcctg
    45901 gtatcaggcc gatggccgct atagcgtgaa gaataaattc ccggcgttta ccggtgaact
    45961 gcccaatggc gatcaatatt atggttttcc ggcagaaaac gacgcgttga agattggcaa
    46021 acataacgga ggccaggtta tccattcagc ggatgaacgt gttccgtttg cggaagtggt
    46081 cagcgatggt tcggaagcct tcccgttctt gcgcaatgta ttgccgggta tcggttgctg
    46141 cctgtacggc gctgcctgca cctatgataa ttcgcctgac gaagatttta ttatcgatac
    46201 cctacccggc cacgataata cactgctcat taccggcctg agtgggcacg gttttaaatt
    46261 tgcgtcagtt ttaggggaaa tagctgccga ttttgcgcaa gacaaaaaaa gcgattttga
    46321 tttgacgcca ttcaggcttt cccgcttcca ataatcatca ttacggcctc cagaatgagg
    46381 ccgtttttac aggagtattt aatgcgtcgg ctgttgcact atctcatcaa taatattcgc
    46441 gagcatctga tgctttatct ttttctctgg ggattgttgg caattatgga tctgatctat
    46501 gtgttttatt tttagttttc cgcactgaca taaactgaca aatatttaat ctccctttca
    46561 tttagcgtga aaaattgaat gagattattc ctgcttctac agcactcacc agacatctct
    46621 gatgctgaac ccactaaact tatgaagatt ctttcattat taattcaaaa ttattgaaat
    46681 aaaaataaat atcattcaat ttgttatttt tgtgttgcaa ttcattctcc gacacgccat
    46741 tttctttctc tcgcaaattc gtattggatg cacttatgtc attcacaaat acccctgaac
    46801 gctatggtgt tatatctgcg gccttccatt ggctaagcgc aattattgtc tacggcatgt
    46861 ttgctttagg tctgtggatg gtcacgctca gttattacga tggctggtat cacaaagcac
    46921 ccgaactgca taaaagcatc ggtattttgt taatgatggg gctggttatt cgcgttctgt
    46981 ggcgtgtcat atctccaccg ccgggtccgc tgccgagtta ttcgcccatg actcgtcttg
    47041 ccgcaaaggc tggacatctc gcattgtacc tattgctgtt cgccatcggc atcagcggct
    47101 atctgatctc aactgccgat ggtaaaccga tcagcgtttt tggctggttt gacgtccccg
    47161 cgacccttgc cgacgctggc gcacaggcag actttgccgg tgccctgcat ttttggctcg
    47221 cgtggagcgt cgtagtactg tccgttatgc acggatttat ggccctgaag catcatttca
    47281 tcgataaaga cgacactctg aagcgcatgc tgggaaagtc gtcatctgac tatggagtat
    47341 aaaaaatgaa aaaaagcctg cttggtttaa ccttcgcgtc cctgatgttc tctgccggtt
    47401 cagcggttgc cgccgattac aaaattgaca aagaaggtca gcacgccttt gttaatttcc
    47461 gcatccagca ccttggctat agctggttat acggcacctt taaagatttc gacggtactt
    47521 ttacctttga cgaaaaaaat ccggctgccg ataaagtgaa tgtgacaatt aacaccacca
    47581 gcgtcgatac taatcacgcc gaacgcgata aacatcttcg cagtgcagat ttcctcaata
    47641 ccgcaaaata tccacaggca acattcacct ccaccagcgt gaagaaagac ggtgacgaac
    47701 tggatattac cggcgatctg acgctgaatg gcgtaaccaa acccgtcacg ctggaagcga
    47761 aattaattgg tcagggcgac gacccatggg gtggtaaacg tgcaggcttc gaggccgaag
    47821 gcaaaattaa gctcaaggac ttcaatatca agacagattt aggtccagct tctcaggaag
    47881 tggatctgat tatttcagtg gaaggcgtac agcagaagta agagtgaaat gttgccggat
    47941 ggcacgcatc cggcaatgat atttattccg ttggatcagg aatgcccagt gttgtattca
    48001 gacgtccacg agacttatta aagatcttat tgccattttc acgtcctgcc cgacggcgtc
    48061 gctgttcttc tggcggtaac gcgctttctt cgcagcaaat ctcactacaa caacctttgt
    48121 atttttccgc acatactgga cactgaataa acagcaggtg gcagccatca tttttacagt
    48181 tggtatggct gtcgcacggc gcgccgcact ggtggcaatg cgcgataatc tcatccgata
    48241 tacgttcgcc catccgctcg tcaaaaacaa aatttttgcc aataaaacgc accggcaagc
    48301 cctgctcgcg cgccttacgg gcgtattcaa taattccgcc ctcgatatgc cagactttgt
    48361 tgaatccgtt atgtttcatc caggcactgg ccttttcaca acgaatgccg ccggtgcagt
    48421 acatgacgat ttttttatct ttatgtgcct gcatcatctc gactgctttt ggcagctgct
    48481 cacggaaggt atctgccgga atttcgagcg cgttttcaaa gtgccccact tcatactcat
    48541 agtggttacg catgtcgata aatagtgcat cgggatcgtc aagcatggcg ttcacttccg
    48601 ccgcttgcag atactcacca acattgctgg catcaaagtg aggatcgtca ataccgtcgg
    48661 caacaatgcg atcgcgtacc ttcatgcgca gtacccagaa agatttcccg tcatcatcca
    48721 acgcgatatt caggcgtaaa ccctctaaag ccgggtcgaa ggcatagagc tgcgcgcgaa
    48781 atgtttcaac attgctcgca ggtacgctga tttgcgcgtt aatgccctca tgcgccagat
    48841 acactcgccc aaaaacattc agcgcggtaa acagctgata taaagcgtca cgggtcgcct
    48901 taggatcggc gatgtggaaa tacttgtaaa acgaaatggt ggttcgcggt tcgctctcag
    48961 ccaacatttt ggcttttagc gcgtcgttgg aaatgcggtt gtgtaacact ggcatggtgt
    49021 acggttcctg cgagatggga aagtaaaaat ccgcggcatg atatagcaat tatcgataat
    49081 taacatccac acattttacg ctacatttgc gcattaaaaa ttatttgtta tttacaagcg
    49141 cggcaatttc gcccagtctt cagccacaaa ttttggttgc gggcgaaaaa atgcgacaat
    49201 acatacaatt gcccgaatag gttgaaaaac aggattgata tgacgaatct acccaagttc
    49261 tccaccgcac tgcttcatcc gcgttattgg ttaacctggt tgggtattgg cgtactttgg
    49321 ttagtcgtgc aattgcccta cccggttatc taccgcctcg gttgtggatt aggaaaactg
    49381 gcgttacgtt ttatgaaacg acgcgcaaaa attgtgcatc gcaacctgga actgtgcttc
    49441 ccggaaatga gcgaacaaga acgccgtaaa atggtggtga agaatttcga atccgttggc
    49501 atgggcctga tggaaaccgg catggcgtgg ttctggccgg accgccgaat cgcccgctgg
    49561 acggaagtga tcggcatgga acacattcgt gacgtgcagg cgcaaaaacg cggcatcctg
    49621 ttagttggca tccattttct gacactggag ctgggtgcgc ggcagtttgg tatgcaggaa
    49681 ccggggattg gcgtttaccg cccgaacgat aatccactga ttgactggct acaaacctgg
    49741 ggccgtttgc gctcaaataa atcgatgctc gaccgcaaag atttaaaagg catgattaaa
    49801 gccctgaaaa aaggcgaagt ggtctggtac gcaccggatc atgattacgg cccgcgctca
    49861 agcgttttcg tcccgttatt tgccgttgag caggctgcga ccacgaccgg aacctggatg
    49921 ctggcacgga tgtccggcgc atgtctggtg cccttcgttc cacgccgtaa gccagatggc
    49981 aaagggtatc aattgattat gctgccgcca gagtgttctc cgccactgga tgatgccgaa
    50041 actaccgccg cgtggatgaa caaagtggtc gaaaaatgca tcatgatggc accagagcag
    50101 tatatgtggt tacaccgtcg ctttaaaaca cgcccggaag gcgttccttc acgctattaa
    50161 atctcccctg ccggatgctt cagaatggca tccggcatta ccacagcaaa tccccctgat
    50221 ttagcgataa aagctctctg gattgcgccc cctggaagtc gggcgcataa ttagtgtgct
    50281 tatcttttct tcttatgttc accgcgcctg gcgcaccaac agcggattgc tatgtcaccc
    50341 tgtgaaaatg acacccctat aaactggaaa cgaaacctga tcgtcgcctg gctaggctgt
    50401 tttcttaccg gtgccgcctt cagtctggta atgcccttct tacccctcta cgttgagcag
    50461 cttggcgtta ccggtcactc cgccctgaat atgtggtccg gtattgtctt cagcattaca
    50521 tttttatttt cggccatcgc ctcaccgttt tggggtggac tcgccgaccg taaaggccga
    50581 aaactcatgc tattacgctc tgccctcggc atgggcatcg tgatggtgtt gatgggactg
    50641 gcacaaaata tctggcagtt tttgatccta cgggcgcttc ttgggttact tggcggattt
    50701 gtccccaacg ctaatgctct tatcgccaca caagtaccgc gtaataaaag cggctgggcg
    50761 ctgggtacgc tctccacagg cggcgttagt ggtgcgttgc tcggcccaat ggctggcggc
    50821 ctgctcgccg atagctacgg cttacgtccg gtattcttta ttaccgccag tgtgctcata
    50881 ctctgctttt tcgtcaccct gttctgcatc agagaaaaat tccagccggt cagcaaaaaa
    50941 gagatgctgc acatgcggga agtggtgaca tcacttaaaa acccgaaact ggtactcagc
    51001 ctgtttgtca ccacgttaat catccaggtg gcgacaggct caattgcccc cattctgacg
    51061 ctgtatgtcc gcgaactggc gggtaacgtc agtaacgtcg cctttatcag tggcatgatc
    51121 gcctcggtgc caggcgtggc ggctctgcta agcgcaccac gactcggcaa acttggcgat
    51181 cgaatcggac ccgaaaagat cctgattaca gcgctgatct tttctgtact gctgttgatc
    51241 ccaatgtctt acgttcagac gccattgcaa cttgggattt tacgtttttt gctcggtgcc
    51301 gccgatggtg cactactccc cgccgtacag acactgttgg tttacaactc gagcaaccag
    51361 atcgccgggc gtatcttcag ctataaccaa tcgtttcgtg atattggcaa cgttaccgga
    51421 ccattgatgg gagcagcgat ttcagcgaac tacggtttca gagcggtatt tctcgtcacc
    51481 gctggcgtag tgttattcaa cgcagtctat tcatggaaca gtctacgtcg tcgtcgaata
    51541 ccccaggtat cgaactgatt tttcgccttt catacttgca aaagcggaga atcagctatc
    51601 cttttccctg aaacctcatc aactcaaagg gagaatcgtg atgaccatgt acgcaacgct
    51661 tgaagaagcc attgacgctg cacgcgaaga atttcttgca gacaaccccg gcatcgacgc
    51721 cgaagatgcg aatgtgcaac agttcaatgc ccaaaaatac gttttgcagg acggcgacat
    51781 catgtggcaa gttgagtttt ttgccgacga aggggaagaa ggtgaatgtt tacctatgct
    51841 tagcggtgaa gccgcgcaaa gtgtttttga tggcgactat gatgagatag agatacgcca
    51901 ggagtggcag gaagagaata cattacatga atgggacgag ggggaatttc agcttgagcc
    51961 accgctggat accgaggaag gacgcgcagc agctgatgag tgggatgaac gttaatcact
    52021 catacgggcc atgatgagtg tcgatcggca gcagtaaagt atcaaaaacc agagagaatg
    52081 gcagatcaag gatcgtgaca taacgccagg cggagtcacg cacatcccat tgcacaccgg
    52141 gataatattg gttgccatgc ccctgccccg gtatagtgcg actaataatg ctgccacagc
    52201 cactcagcaa ggtcaccatg atgctcacca caattaaacg catttgatac ctctttcgta
    52261 aaaaaatacc ggcataacgc cggtattttt attggctcgt tgtttttgcc ggatgcggcg
    52321 tgaacgcctt atccgaccca caaaatcttg caaaatcaat aaattgcagg aacaatgtag
    52381 gcctgataag cgtagcgcat caggcaacta cgtttttatt gcgaagccgc atccggttta
    52441 cgcaatgcca gtggattgag ctttatccct tcgtaataac tcacccatga agaatatctc
    52501 tccggggaat tccagacgcg gaaatgcaga cgggccatcg tcaccggatc gcttagcagc
    52561 accaggcgac gatcacgatt cagcttctct ggcgtctcgt tcagcgcctg ttcaacgtgg
    52621 cggtcacggg cgatttccag caccttactg gcgcggtgac gcgcggtcgc cattgcggtt
    52681 gccagagcgt taaatgacgg gttaaacact gcgtgcataa agccatcatc aagggagcgt
    52741 tgacgattca tctcaaggaa ccgatcggta tcaaccagca cctgcggcgg cgaatactct
    52801 tccgggatca ggaacagttt ccagcgtttg gtgcgcagac caacggtggc acggctggaa
    52861 atcaccgaaa caaacggtga caggatcaac gagaagacaa tcggtgccag ccagaacagg
    52921 aaacgcagat ccagccacgc catcccaaca gcccacacta accctaacag cagctgtgag
    52981 ccgtggcgtt tgaacgcttc accccaggaa gtggagtcat catcacgctg cggtgaattc
    53041 cacaccactt cccagccaag gaacgcgctg acaacgaaga ccgtatggaa cagcatgcgt
    53101 accggagcca gcagcacgga aaaaagcact tccagcagca acgataatgt aacgcgccag
    53161 aagccgccgt attctttcgt tcctttgcac cagataagca aaatgctcaa taacttcggc
    53221 aggaacaaca gcaccatagt cgaagcaaaa agtgcaatcg ccagctcagg acgccactgc
    53281 ggccacaccg ggaacaactg ccgtggttgc agaaagtatt gcggttcggt caacgcatgt
    53341 actacctgca atgcagtaga gagcgcgagg aacataaacc acagcggagc ggagagataa
    53401 gacatcacgc ccgtcaggaa caccgcacgg tgaaccgggt gcataccctt aaccaggaac
    53461 agacggaagt tcatcaggtt accgtggcac cagcggcggt cacgttttag ctcatcaagc
    53521 aagttaggcg gtaattcttc ataagaaccc gggagatcgt aagcaatcca gaccccccaa
    53581 cctgcacggc gcatcaacgc cgcttctaca aagtcatgtg acaggattga accggcaaag
    53641 gaaccttcgc ccggcagcgg agccagtgcg cagtgctcga taaacggttt cacgcggata
    53701 atcgcgttat gtccccagta gtgcgactcg ccaagttgcc agaagtgcaa accggctgta
    53761 aacagtggcc catacacgcg ggtcgcgaac tgctgacagc gcgcatacag cgtatccata
    53821 ccggacgctt tcggcgacga ctgaatgatc ccggcgttcg ggttggcttc catcaggcgc
    53881 accagcccgc acaaacaatc accggtcatt accgagtcag catccagcac caccatgtag
    53941 ctgtactggc tgccccagcg acggcagaag tcatcgatat taccgctttt acgcttcacg
    54001 cggcgacggc ggcggcgata gaaaatctga ccttcgccac cgacttcagc gataagctcc
    54061 atccaggctt tttgctctgc gacgcagata tccgggttat aactgtcact aagaatgtag
    54121 acatcaaagt gtttggcatt cccggtggct tttactgatt cccacgttgc acgcaggcca
    54181 gcaaaaacac ggttcacgtc ttcgttacag ataggcatga tcaacgccgt gcgatgctcc
    54241 gggtttaatg gttcatcgcc aactgttgac gcagatatac tgtatttatc gcgaccaata
    54301 agcagttgca ggaagcccat taacgccgtc cagaatccgg cggacaccca acagaacagt
    54361 accgcaaaga ggatcaggat accggtttgc agcatataag gcagaagctg cataaaggaa
    54421 acccacaaat cctgaccaac catatccata ggattaatca gcgcccaacc ctgataagga
    54481 agaatggtct tcatatacca ggtcgcgacg acagtttgcg cgagcgtcag gatcaacaga
    54541 atgtaacggc ggatggtacc gacggtacgc cacttttgct cgctctcctg ctcttctttg
    54601 gtcaaacgag ccagatagcg cggcgtgaca tcgcgtccac gcaggcgatc ccagaaacgg
    54661 cctaccgggt tggtacgcca cgggtcggga aacatcgagg agcgttttgc ttctggcatc
    54721 gccttcagct gatcgcgccc ttcgtcgtct ttaattaact gtccatcagc aagtgaatct
    54781 ggccaggctt gttccagacg cgcctttacc gagccttgcg gggaatcatc ctcccgcgcc
    54841 caggtgcggt gttcggcatc cagcgcctga tgaacggcgc ggatatcagt cttcggcaat
    54901 gccgctttct cgcttgcggc gatgggcatt gcgtcaatgt actcagttgt cttattcatt
    54961 ggcaggtaac tggtagctcc aggtttcact caacgtctga tcggcattca ccagcgcagc
    55021 acgcatttca gtggttttct tggcatcttt cactttcaca cgcatcacca gacgccagcc
    55081 tttggtaacc gggttatagc gcaccgtgct ttcaactatc tcaccattat caccaatgct
    55141 ggtttgcgct gtgaccgggg tatcctctgg cagttttttc atctctgcgc cggtaaaatc
    55201 gaccacaaag gcgatagtac cgtcaggctg gcgaatcagg ttcgactgct tcacatcccc
    55261 cgttgaacga cgcgtttgtt gcacccatgc gttatctggc gcatgcagtt tgtcttcatc
    55321 acggctgaag gtgatggtgt atttaaagtt catctcttta cccggctccg gcagctgatc
    55381 cggcgtccag taagcgacga tgttatcgtt ggtttcatcg ttggttggaa tttccaccag
    55441 ctcaacgctg cctttacccc actccccttt cggagtcacc catgcgcttg gacgaagatc
    55501 gtaacgatca tcgagatctt caaagcggga gaaatcacga ccgcgctgca acagaccgaa
    55561 gccttgcggg ttttccatag agaagctgct gaccgctaaa tgtttcgggt tattcaacgg
    55621 acgccagatc cattcgccat taccggcatg gatagagaga ccgttagagt cgtgcaactc
    55681 cggacgatag ttatttgcag gcgacggttg gttcggccca aacaggaaca tactggttaa
    55741 cggtgcaacc cccagtttgc cgactttatc gcgcagatag attttcgact gcacatccac
    55801 aaccgtgtca cgccctggca taactacgaa tttgtaagca cctgtcgcgc gcggcgagtc
    55861 aagcaatgca taaatggtta aacgtttatc agtcggtttt ggacgctcga tccagaactc
    55921 tttgaagcgt ggaaattctt cacccgatgg caaggcggta tcaattgcca ggccgcgggc
    55981 agaaaggcca taaacctgac ctgcaccaat cacgcggaaa tagctggccc cgagcatgct
    56041 gacgatttca tcgtttttat ctttgctgtt gatcgggtaa agcactttaa aaccggcaaa
    56101 accaaggtct tttaccgtgt ctttgtcatg ctgaacatcg ccgaaagtga aataatccgg
    56161 gctgtatttg attcgtttga ctgcggtggc agtcacttca tttattttga ccggggtatc
    56221 gaagtacata ccctgatggt agaactcgag tttgaatggg gtcttcagat tgttccagta
    56281 cgctttgtca tgattaaact ggatctgctg atagtccgcg tatttcatat cgcggaaaac
    56341 ggagggcaag ttgcttttgg gcgcctcata gcctttcccg gctaaggatt gagcttgctt
    56401 tgcgacatca tcaatactga aagcccagct tgaagatgta tacagggtta acattactgc
    56461 agcactcaac caacgcattt tcatcatttg tagtttatgt ttcataatta gtaagcactt
    56521 ccccctttgt gtgcttatat cgatccgatc tattttaatg gataaccagc cattccgaca
    56581 acagaatccc ggtatcgttc agcacgctgg ctcatggatt aagcacctga ttgcaaccgt
    56641 tttcactttc cgtgcttctc ctaaagacag aagcatcgac ttcgtgtagg gtttgccagc
    56701 aattttattt atcaaccgtc ttacagacgc ttccattctg gttagtggta gcgatgatgc
    56761 tatgcataaa aaccttgaag cctgcgcctg gcgcggtcag gtcggtgtcg ctatggcaac
    56821 aagcctaaag gcataacccg acagggataa gacgaaaaat ccgagattag ttaaccatat
    56881 atgaatccag tacccgcgca acgtgaatat ttcctcgact ccatccgcgc ctggctgatg
    56941 ttgttaggga tcccttttca tatttcttta atctattcga gccatacatg gcatgtgaat
    57001 agcgccgaac cgtcattgtg gctgaccctt tttaatgact tcatccactc gttccgcatg
    57061 caggtatttt tcgttatatc cggctacttt tcctacatgc tttttttacg ctatcccttg
    57121 aaaaaatggt ggaaagtacg tgtcgaacgt gtaggtatcc cgatgttaac agccatcccc
    57181 ttactgacat tgccgcaatt tattatgctg caatacgtca aagggaaagc ggaaagttgg
    57241 cctgggctgt cattgtatga caaatataat acgttggcct gggaattaat atcacatctg
    57301 tggtttttac tggtgttagt ggtcatgacg acgctgtgcg tatggatatt taaacgcatc
    57361 agaaataatt tagaaaattc tgataaaacg aataaaaaat tctcgatggt aaaactatcg
    57421 gtgatttttt tatgcctcgg catcggttat gcggtaataa gaagaacgat ttttattgtg
    57481 tatccgccca ttctgagtaa tggcatgttc aattttattg tcatgcaaac gctgttttat
    57541 ttgccgttct ttatcctcgg cgcactggct ttcattttcc ctcatcttaa agccttgttt
    57601 accacgccgt ctcgtggctg tacccttgca gcagcattgg cgtttgtcgc ttatttactc
    57661 aaccagcgct atggcagtgg cgatgcctgg atgtacgaaa ccgagtcggt gatcaccatg
    57721 gtcctcggtc tgtggatggt gaatgtggtc ttctcctttg gccaccgttt gcttaacttc
    57781 cagtcagcgc gggtgactta ttttgttaac gcatcgctgt ttatctatct ggttcaccac
    57841 ccgttaacgc tgtttttcgg cgcatacatt acaccgcaca tcacctccaa ctggcttggt
    57901 tttctctgtg gcctgatatt tgtagtaggg attgcgataa ttctgtatga aattcatttg
    57961 cgcatcccgt tactgaagtt tttgttctct ggtaaaccgg ttgttaagcg tgagaacgat
    58021 aaagcaccag cccgttaagc cacatttaca ataaccattc cacgggcaat atcgacgcca
    58081 gtctgaccat aacccgcttc cagaaactgg tggcgggttc ttttttgaga ataatctctt
    58141 tctttgcatg acgatcaacc cagttgatcc gtccccacct gtccagacgg agctgccagg
    58201 ccgcatcata ctggctctga ataaagcgtt tatcaattaa ctgtgccagc gtttcgctct
    58261 ctatcacgaa gcccatttca gtattgagca atgttgaacg cggatcgaaa ttgaaagagc
    58321 cgataaacac cgttttacca tcgatgctaa aggttttagc atgcaggctg gctccggaat
    58381 taccggttat gccgcgatcg tgtaacgtac tactttgttc acgcgtcggc ttgagttcat
    58441 ataattccac gccatagcgg agcaattttt tgcgccagcg cgcgtatccg gcatggacga
    58501 cagcaacatc gttagcggca agagaattgg ttaggatcgc aatctttacc ccttttctca
    58561 ccatccgtaa gagttgcgcc acacctgcgc gtgtcggtac aaaataggaa gagataatat
    58621 cgatgcgttc actgggtgag cccatgatat cgaacaggcg ctgcggtaac agtgaatgcc
    58681 gttttgcctt gccctcccct ttcgccggat catcacttaa taaacgtgtt ttcgcccaga
    58741 taagcggcaa cgttccatca accagatgat ttataaacgg actggattcc attttgcgta
    58801 agtaacgatg cgtcatagca tcgttatgcc aggaggcggg taactcgatg cgatccgcca
    58861 tttcaccttc cgggacatcc agcacttgct gtaagggtga aaccgatttg caataccagt
    58921 agcgggcgaa atcatcggca acgtcctcta ccacgggccc tattgccata acatctaaat
    58981 ccgaaaaaag tggctcttcc cctgccccaa aataggcatc accaatattt cgccccccta
    59041 ccagcgtcac cacgccatcg acagtgaaac ttttattgtg catacggcga ttgagacggg
    59101 aaaagtcggt gatataacca agcggacgca gcaagcgaaa cgagaaagga ttaaaaagcc
    59161 ggacttcaat gcggggatgg ctgtcaagca agcgtaaaat gtcgtcaagt ccgggcgtat
    59221 tgttgtcgtc cagcaacaaa cggacgcgaa cgccacgctt tgctgcggct aacagggcgg
    59281 aaaacagtaa ccgacccgac atatcgtcct gccagatgta atactgaaca tccagcgtat
    59341 gctctgccat ttcggccaga cgataccggg cggcaaacgc atccagactt ttctccagag
    59401 ggaaaagacc acactgaccg ggatgttgcg aacacagtgg cagcaccgcg ctcgccagcc
    59461 ggggcaaatc attcatctcc ttgttgggta aggagtcttt cgtagaggtg ggcgttttct
    59521 tcatcataac agacaaagta tacctgttcg ggtaaagcgt gacgggtaat aaattctgaa
    59581 acggttttaa ctgcgatttc agccgctgcc gcacgagggt aaccataaac cccagtactg
    59641 attgcaggaa aagccactga cgtatagctg tttgccgcca ccagtcgtag gctattgaga
    59701 taggcatcct gcaaaagctg gtcttcgttt tgttcaccac cacgccagac tggcccgacg
    59761 gtgtgcacta cggctttagc gggaagagcg cctgcaagcg taataacggc atgccccgta
    59821 gggcaatcgc cctgctgttg cctgactttt aaacaggcat ccagcagggc cggacccgct
    59881 gcgcgatgaa tggccccatc gacgccgccg cctcccatta atgacggatt agccgcattc
    59941 acaatcacat caacggccag tttggtaata tcaccctgca caacatgaat acgcgttttc
    60001 ataacgcctc cttacacctt cttgtttctt aagtgtacag caggcttttt ggttaaatcg
    60061 catttttctg ctcagcaacg tgcgttgttc agaaacttag ttatattcga tgttcattac
    60121 agccagcttt ttctgtttat caagatactg cacttttact gaggctattt gcgcgttttt
    60181 aacattaccg ctcattaacg ctttgctgga ataggttttg cccggaacag aaccattctg
    60241 tgggcagttc atttctacgg gtgatgactg ggtgctgacg tcacatgccg gttccacaat
    60301 ttggccataa aaatggatca cccccccttg cgcggtgctt cctgcgatgg caataaaagg
    60361 aaaaagcaaa ctgccgagca taagagagtt taaaaaaggt ctgaacattt attctatttc
    60421 ctcaatgatt agtcatcctt gagggttgtg ttatccatac tttcaggttg gctgattcgc
    60481 agcagaccat tctctccaga ttcatcttat gctcgatatt tcaacaaatt aagacttttc
    60541 tgaagagggc ggccattgtt gtgataaatg aagtgactgt ccatcagaaa cagtaacaac
    60601 tattttcacc cgatcttccg gggaaatatt taaactcaac ttcgtcaaag caataggttg
    60661 attagcaggc aatgagaggg tcttttcttg cttcgtctga ctttgccctg aactgccttc
    60721 gcgcagggac aatatttgta ctctgcacag acaagattga gtaagagtga cttcaggaat
    60781 aatggtatac acatcccctt gctgggtcgt attaaaggtt atctggctgg aaagtgccgc
    60841 aaggagtaat aacgcattca tattcttctc ccgaaaaaaa acagggcttg cgccctgttt
    60901 ctgtaataca aatgatgtat tagtactgat gagcggtcgc gttgttacca aagccaacct
    60961 gagtcacgtt gacggaggag ttagatgcag tctggtcaac tgcagcaccg ttgccaccac
    61021 cgaactgttt aacagtcatt tcagaatttt tgccgttcca ctgatcaaga gtggcgctgt
    61081 taccgaagcc acgttgggtc agatcgattg agctgtcatc tgagccctga cctacatctg
    61141 caccattacc accgccatgc tgggtaatag tcaagtcaga gttacgggca tcagtttgca
    61201 gagcaagtgc agagttaccg ccaccgtact ggtaaatgtt cagctctgaa tttgggccgc
    61261 tattattacc gccaccaccg tggttaccgc cgccgccgta ctgaggaaca acacctgcca
    61321 gagcgctacc ggagaatacg attgctgcaa ttgctgctac ttttaaaagt ttcatgtaaa
    61381 acccccatcg gattgattta aaagtcgaat ggaaattaac gttgtgtcac gcgaatagcc
    61441 atttgcgact gtctctgcac tacaattgcc gttttttgag taccatactg tgtaatattt
    61501 gctttattac cagaaccttt ctggataatc atcgcagtat taccataagc accttgcgaa
    61561 atactggcat cgttggcact gcccgcctga tcaatatatg caaggttata atctcctgtc
    61621 tggtcaatct ttgcccggtt gctactacct tcttgcgcaa caaccgccaa aagttttgag
    61681 cctccctgcc gtaactgagc actattattc gtcccagctt gaccaattat ggctgcctga
    61741 ttaaatgaag acttactcaa ttcatttacc gcgaagttat attctgaatt agctaaatca
    61801 taacctgctg cggctgcaat cccaggcgca cccagtattg ttaacatcat aaataacaat
    61861 ttgtttttca tgttgtcacc ctggacctgg tcgtacattt aagaaattaa atcatttcaa
    61921 cttggttgtt aacgcaacct gtattttgtt aacgctgcgt tacgatggaa agtatgtctg
    61981 cggaaatatt tttaataact cacccgcgcg ttgtattttt ctttttttgt tcataccaaa
    62041 gtattaaaac ccacgtaaat acgctgttat ctacgcaaaa aatattttgt tttcttttaa
    62101 atctccgttt tccgctatca aaaatcacca gacagtcatt tttcttgccc gtcgctgatt
    62161 gctgcgatat gtcttgcgca caagccgtga caaacctcgt ctttttgcag taaaaaattg
    62221 tccacggagg tgcggagaaa aaac
//
LOCUS       DAZGSP010000014        60559 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZGSP010000014 DAZGSP010000000
VERSION     DAZGSP010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.014"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            37..655
                     /locus_tag="ACHK74_001022"
                     /pseudo
     CDS             37..655
                     /locus_tag="ACHK74_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237102.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            758..1261
                     /gene="yfbM"
                     /locus_tag="ACHK74_001023"
     CDS             758..1261
                     /gene="yfbM"
                     /locus_tag="ACHK74_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFP0219883.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1328..2785)
                     /gene="nuoN"
                     /locus_tag="ACHK74_001024"
     CDS             complement(1328..2785)
                     /gene="nuoN"
                     /locus_tag="ACHK74_001024"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFP0219884.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2792..4321)
                     /gene="nuoM"
                     /locus_tag="ACHK74_001025"
     CDS             complement(2792..4321)
                     /gene="nuoM"
                     /locus_tag="ACHK74_001025"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFP0219885.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4485..6326)
                     /gene="nuoL"
                     /locus_tag="ACHK74_001026"
     CDS             complement(4485..6326)
                     /gene="nuoL"
                     /locus_tag="ACHK74_001026"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFP0219886.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEIISGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6323..6625)
                     /gene="nuoK"
                     /locus_tag="ACHK74_001027"
     CDS             complement(6323..6625)
                     /gene="nuoK"
                     /locus_tag="ACHK74_001027"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFP0219887.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMHG"
     gene            complement(6622..7176)
                     /gene="nuoJ"
                     /locus_tag="ACHK74_001028"
     CDS             complement(6622..7176)
                     /gene="nuoJ"
                     /locus_tag="ACHK74_001028"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFP0219888.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7188..7730)
                     /gene="nuoI"
                     /locus_tag="ACHK74_001029"
     CDS             complement(7188..7730)
                     /gene="nuoI"
                     /locus_tag="ACHK74_001029"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFP0219889.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7745..8722)
                     /gene="nuoH"
                     /locus_tag="ACHK74_001030"
     CDS             complement(7745..8722)
                     /gene="nuoH"
                     /locus_tag="ACHK74_001030"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFP0219890.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8719..11445)
                     /gene="nuoG"
                     /locus_tag="ACHK74_001031"
     CDS             complement(8719..11445)
                     /gene="nuoG"
                     /locus_tag="ACHK74_001031"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFP0219891.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFRSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11498..12835)
                     /gene="nuoF"
                     /locus_tag="ACHK74_001032"
     CDS             complement(11498..12835)
                     /gene="nuoF"
                     /locus_tag="ACHK74_001032"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFP0219892.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12832..13332)
                     /gene="nuoE"
                     /locus_tag="ACHK74_001033"
     CDS             complement(12832..13332)
                     /gene="nuoE"
                     /locus_tag="ACHK74_001033"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFP0219893.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13335..15137)
                     /gene="nuoC"
                     /locus_tag="ACHK74_001034"
     CDS             complement(13335..15137)
                     /gene="nuoC"
                     /locus_tag="ACHK74_001034"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFP0219894.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15231..15893)
                     /gene="nuoB"
                     /locus_tag="ACHK74_001035"
     CDS             complement(15231..15893)
                     /gene="nuoB"
                     /locus_tag="ACHK74_001035"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFP0219895.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15909..16352)
                     /gene="nuoA"
                     /locus_tag="ACHK74_001036"
     CDS             complement(15909..16352)
                     /gene="nuoA"
                     /locus_tag="ACHK74_001036"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFP0219896.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16983..17921)
                     /gene="lrhA"
                     /locus_tag="ACHK74_001037"
     CDS             complement(16983..17921)
                     /gene="lrhA"
                     /locus_tag="ACHK74_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFP0219897.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18841..20058
                     /gene="alaA"
                     /locus_tag="ACHK74_001038"
     CDS             18841..20058
                     /gene="alaA"
                     /locus_tag="ACHK74_001038"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFP0219898.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20142..20741
                     /gene="yfbR"
                     /locus_tag="ACHK74_001039"
     CDS             20142..20741
                     /gene="yfbR"
                     /locus_tag="ACHK74_001039"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP0219899.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20800..22632)
                     /gene="yfbS"
                     /locus_tag="ACHK74_001040"
     CDS             complement(20800..22632)
                     /gene="yfbS"
                     /locus_tag="ACHK74_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFP0219900.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22719..23369)
                     /gene="hxpA"
                     /locus_tag="ACHK74_001041"
     CDS             complement(22719..23369)
                     /gene="hxpA"
                     /locus_tag="ACHK74_001041"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFP0219901.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLSHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23380..23874)
                     /gene="yfbU"
                     /locus_tag="ACHK74_001042"
     CDS             complement(23380..23874)
                     /gene="yfbU"
                     /locus_tag="ACHK74_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFP0219902.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23957..24412)
                     /gene="yfbV"
                     /locus_tag="ACHK74_001043"
     CDS             complement(23957..24412)
                     /gene="yfbV"
                     /locus_tag="ACHK74_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFP0219903.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24750..25952
                     /gene="ackA"
                     /locus_tag="ACHK74_001044"
     CDS             24750..25952
                     /gene="ackA"
                     /locus_tag="ACHK74_001044"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFP0219904.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26027..28171
                     /gene="pta"
                     /locus_tag="ACHK74_001045"
     CDS             26027..28171
                     /gene="pta"
                     /locus_tag="ACHK74_001045"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP0219905.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVARLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28361..29881
                     /gene="yfcC"
                     /locus_tag="ACHK74_001046"
     CDS             28361..29881
                     /gene="yfcC"
                     /locus_tag="ACHK74_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFP0219906.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29914..30456)
                     /gene="yfcD"
                     /locus_tag="ACHK74_001047"
     CDS             complement(29914..30456)
                     /gene="yfcD"
                     /locus_tag="ACHK74_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFP0219907.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30514..31065)
                     /gene="yfcE"
                     /locus_tag="ACHK74_001048"
     CDS             complement(30514..31065)
                     /gene="yfcE"
                     /locus_tag="ACHK74_001048"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFP0219908.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAELLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31121..31765)
                     /gene="yfcF"
                     /locus_tag="ACHK74_001049"
     CDS             complement(31121..31765)
                     /gene="yfcF"
                     /locus_tag="ACHK74_001049"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFP0219909.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31901..32548
                     /gene="yfcG"
                     /locus_tag="ACHK74_001050"
     CDS             31901..32548
                     /gene="yfcG"
                     /locus_tag="ACHK74_001050"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFP0219910.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32605..32967
                     /gene="folX"
                     /locus_tag="ACHK74_001051"
     CDS             32605..32967
                     /gene="folX"
                     /locus_tag="ACHK74_001051"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFP0219911.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKDEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32988..33881
                     /gene="yfcH"
                     /locus_tag="ACHK74_001052"
     CDS             32988..33881
                     /gene="yfcH"
                     /locus_tag="ACHK74_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFP0219912.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33929..34831)
                     /gene="rpnB"
                     /locus_tag="ACHK74_001053"
     CDS             complement(33929..34831)
                     /gene="rpnB"
                     /locus_tag="ACHK74_001053"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFP0219913.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLD
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(35028..35801)
                     /gene="hisP"
                     /locus_tag="ACHK74_001054"
     CDS             complement(35028..35801)
                     /gene="hisP"
                     /locus_tag="ACHK74_001054"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFP0219914.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVSGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35809..36525)
                     /gene="hisM"
                     /locus_tag="ACHK74_001055"
     CDS             complement(35809..36525)
                     /gene="hisM"
                     /locus_tag="ACHK74_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0219915.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36522..37208)
                     /gene="hisQ"
                     /locus_tag="ACHK74_001056"
     CDS             complement(36522..37208)
                     /gene="hisQ"
                     /locus_tag="ACHK74_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFP0219916.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37298..38080)
                     /gene="hisJ"
                     /locus_tag="ACHK74_001057"
     CDS             complement(37298..38080)
                     /gene="hisJ"
                     /locus_tag="ACHK74_001057"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFP0219917.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38301..39083)
                     /gene="argT"
                     /locus_tag="ACHK74_001058"
     CDS             complement(38301..39083)
                     /gene="argT"
                     /locus_tag="ACHK74_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFP0219918.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39349..39918)
                     /gene="ubiX"
                     /locus_tag="ACHK74_001059"
     CDS             complement(39349..39918)
                     /gene="ubiX"
                     /locus_tag="ACHK74_001059"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFP0219919.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40013..41530)
                     /gene="purF"
                     /locus_tag="ACHK74_001060"
     CDS             complement(40013..41530)
                     /gene="purF"
                     /locus_tag="ACHK74_001060"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFP0219920.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41567..42055)
                     /gene="cvpA"
                     /locus_tag="ACHK74_001061"
     CDS             complement(41567..42055)
                     /gene="cvpA"
                     /locus_tag="ACHK74_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFP0219921.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42314..42976)
                     /gene="dedD"
                     /locus_tag="ACHK74_001062"
     CDS             complement(42314..42976)
                     /gene="dedD"
                     /locus_tag="ACHK74_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFP0219922.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42966..44234)
                     /gene="folC"
                     /locus_tag="ACHK74_001063"
     CDS             complement(42966..44234)
                     /gene="folC"
                     /locus_tag="ACHK74_001063"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFP0219923.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVISIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44304..45218)
                     /gene="accD"
                     /locus_tag="ACHK74_001064"
     CDS             complement(44304..45218)
                     /gene="accD"
                     /locus_tag="ACHK74_001064"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFP0219924.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45374..46033)
                     /gene="dedA"
                     /locus_tag="ACHK74_001065"
     CDS             complement(45374..46033)
                     /gene="dedA"
                     /locus_tag="ACHK74_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP0219925.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46116..46928)
                     /gene="truA"
                     /locus_tag="ACHK74_001066"
     CDS             complement(46116..46928)
                     /gene="truA"
                     /locus_tag="ACHK74_001066"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP0219926.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46928..47941)
                     /gene="usg"
                     /locus_tag="ACHK74_001067"
     CDS             complement(46928..47941)
                     /gene="usg"
                     /locus_tag="ACHK74_001067"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP0219927.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEACDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48007..49143)
                     /gene="pdxB"
                     /locus_tag="ACHK74_001068"
     CDS             complement(48007..49143)
                     /gene="pdxB"
                     /locus_tag="ACHK74_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFP0219928.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49242..50237
                     /gene="flk"
                     /locus_tag="ACHK74_001069"
     CDS             49242..50237
                     /gene="flk"
                     /locus_tag="ACHK74_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFP0219929.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50234..51412)
                     /gene="yfcJ"
                     /locus_tag="ACHK74_001070"
     CDS             complement(50234..51412)
                     /gene="yfcJ"
                     /locus_tag="ACHK74_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0219930.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51705..52925)
                     /gene="fabB"
                     /locus_tag="ACHK74_001071"
     CDS             complement(51705..52925)
                     /gene="fabB"
                     /locus_tag="ACHK74_001071"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFP0219931.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53084..55090
                     /gene="mnmC"
                     /locus_tag="ACHK74_001072"
     CDS             53084..55090
                     /gene="mnmC"
                     /locus_tag="ACHK74_001072"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFP0219932.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWNEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSHTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55211..55489)
                     /gene="yfcL"
                     /locus_tag="ACHK74_001073"
     CDS             complement(55211..55489)
                     /gene="yfcL"
                     /locus_tag="ACHK74_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFP0219933.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(55523..56071)
                     /gene="epmC"
                     /locus_tag="ACHK74_001074"
     CDS             complement(55523..56071)
                     /gene="epmC"
                     /locus_tag="ACHK74_001074"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFP0219934.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKSQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56071..56880)
                     /gene="yfcA"
                     /locus_tag="ACHK74_001075"
     CDS             complement(56071..56880)
                     /gene="yfcA"
                     /locus_tag="ACHK74_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP0219935.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGIN"
     gene            complement(56880..57704)
                     /gene="mepA"
                     /locus_tag="ACHK74_001076"
     CDS             complement(56880..57704)
                     /gene="mepA"
                     /locus_tag="ACHK74_001076"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFP0219936.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57708..58793)
                     /gene="aroC"
                     /locus_tag="ACHK74_001077"
     CDS             complement(57708..58793)
                     /gene="aroC"
                     /locus_tag="ACHK74_001077"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFP0219937.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58828..59760)
                     /gene="prmB"
                     /locus_tag="ACHK74_001078"
     CDS             complement(58828..59760)
                     /gene="prmB"
                     /locus_tag="ACHK74_001078"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP0219938.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            59926..60477
                     /gene="smrB"
                     /locus_tag="ACHK74_001079"
     CDS             59926..60477
                     /gene="smrB"
                     /locus_tag="ACHK74_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFP0219939.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
BASE COUNT        14980 a        16558 c        15282 g        13739 t
ORIGIN      
        1 ttgataattt acatatgaaa aaaatttcct tttgttatat tatttctgtt ttcactccct
       61 ctgattattt tttaccaacc ctgggtcact gcattatcgc caatgccccg acacgtaagt
      121 cctgagcaat tagaaaaaac agttcgttat cttacacaaa ctgtgcatcc acgtaatgcc
      181 ggcaatattg ataatctgaa taggtccgta gagtatataa aagaggtctt tgtcagtagc
      241 ggtgccagag ttaccgcgca gggcgtcccc gattggctat tacgactctg cgcctggaag
      301 ccagaattac ccttacccgg caatgtcctg gctttatccc gatcggggag actttattgc
      361 cgtagtcggc agaatgcagg atatcaacgc cgttcgtcag gtaaaatcgg cattgttgtc
      421 atcccgggat ttatctgttt attctatgaa tgccccaggg tttattcccg gtattgattt
      481 ctctgatcac cttaataatt ggcaacacga tattcccgcc gtaatgatta ctgacaccgc
      541 tttttatcgt aataaacaat accacttgcc cggtgatacc gcagacagat tgaattatca
      601 gaaaatggct caggtggtgg atggtgttat aaacttgtta tacaacagta aataataaaa
      661 atttctcgag gcgggtccag aaaaagcgac atgcacattt tattttccct cgtctgtttt
      721 aagatgcgaa acatgaaatt caatggagga aaaataaatg ggaatgattg gctattttgc
      781 agaaattgac tcagaaaaga ttaaccaact gcttgagtct actgaaaaac ccttaatgga
      841 caatattcat gacacgcttt caggattacg ccgactggat attgacaaac gctgggattt
      901 tttacacttt gggctgacag gcacctctgc ttttgatccc gcgaagaatg atccgctgag
      961 tcgtgccgtg cttggtgaac acagcctcga agatggcata gatggcttcc tgggattgac
     1021 ctggaatcag gagttagccg cgacaatcga ccgactggaa tcactggatc gaagtgagct
     1081 gcgtaaacag ttttccatta agcgactcaa tgaaatggag atttatccgg gagtgacctt
     1141 ttcagaagag ttagaggggc aactctttgc ttcgatcatg cttgatatgg aaaagctgat
     1201 tagcgcgtat cgacgtatgc tgcgacaagg gaatcatgcg ctgacggtta ttgttgggtg
     1261 atggtgtcag ggcaccaaaa aaaaccgccg aacatgtcgg cggctttctg acttacaaac
     1321 tgatagctta catcagcggc attgctaacc gcacaatgct aatcagcggt tgtggccata
     1381 cacccagcac cagtaccaac agtgcagaga tcagcaccac gataccgcct gcgctgtact
     1441 gccagtttga cggtgcatcg cgacccggtt gttccggggc gtgcaggtac aggctcaccg
     1501 ccacgcgcag gtagtagtaa aggccgattg ccgaaccgac aaccacggcg cccaccagcc
     1561 accacaagtg tgcctggaca ccgaccgcca gcacgtagaa cttaccaata aagcccagcg
     1621 tcatcggaat accggccaga gacagcatca tcaccgtcat caccgccgcg aggatcgggc
     1681 gatgccagaa cagaccgcgg taagagaaca gggaatcagc atccgggcca cgatacgggc
     1741 tggacatcag gctgaccacg ccgaacgcgc cgaggctgct gaacagataa ccggccaggt
     1801 aaacccctac cgcttccatc gacatctcac cagtttgcag cgcaatcagc gccaccagca
     1861 gatagccgag gtgagagata gatgagtaac cgagcagacg tttgatgttg gtctggctca
     1921 gcgccatcag gttaccgaag atgatggagg caaaggcgat aatcgccagt actacgcgaa
     1981 tcgcttcgct gtcacccacc ggtgcgtaga ggaacagacg catcaccaca ccgaagatag
     2041 cgattttgct cgccgtcgcc aggaaagtgg aaaccggcgc aggcgcgccc tggtatacgt
     2101 ctggcgtcca caggtggaac ggcaccagcg agagtttgaa gccgaggcca acaatcatca
     2161 gtccgaaacc tgccagcaac agcggctcgt tgagcatacc gtcgccgagg tttttaccca
     2221 gcgcgacaaa cgacaggtcg ccagactgtg catacaccag cgccatacca aacagcagga
     2281 aagaagacgc tgcggcagaa aggatggtgt atttgatact ggcttccagc gaacgtttct
     2341 ggcggaaagc gtaaccgacc aggccaaaca gcggcaaaga gatcagttca ataccgagga
     2401 acaaagacgc cagatggttg gcattcgcca gcaggatccc gcccagcgcg gcaattaaca
     2461 ccaacaggta gaactcatcc ttgttgtcgt tatagccttc aagccacggg taggcgaaag
     2521 tacaggtggc gaggctcgcc aacaatacca gcccggtgta aagcatggcg aaaccatcaa
     2581 cgcgcatcag cggcgtaacg tccatagcgc ccgcctggcc gacaaaccag agcgaaacca
     2641 gcgccgcgtt aagcccaata accgagagcg tagcgttgag gaaatgattg cgtcgccacg
     2701 caatggagag catcacaacc accaccgtca agccgacgat cagcaacggt agcagtgcga
     2761 tcaggttttg tggagttatt gtcatggcga tttacggcct tgtagtagta acggaattaa
     2821 caaaccactg ctggatattg ccaatcgcgg agtgcgaggt atccagaatc ggctgcggat
     2881 agaagcccag cagtaccagc agcaccacca gcaacaggat cataaacagc tcacgcagcg
     2941 acatccctgg cagttcctgg ctggcaatct ggcttttcgc tttaccgaag taagcgcgat
     3001 gcaacatcgc cagcgaataa acagatgcaa agaccagccc aaaggtagag ataacggtaa
     3061 tcaccgggac aacctggaag ctgccgaaca gaatcataaa ttcgccgacg aagttaccgg
     3121 tgccaggcat cccaagcgtt gccaccgcaa agaacagcga cagtgctggc agccatttca
     3181 tcttgctcca cagaccgccc atcatgcgca tgtcgcgggt atggatacgt tcataaagct
     3241 gaccacaaag aataaacaga cccgccgccg acaagccgtg cgcaatcatc tggattaccg
     3301 cgccctggta ggccaactgg ctgccggtgt agatagcaat cagcacgaag cccatgtggg
     3361 aaaccgaggt gtaggcgatc agacgtttga tatcggtctg ggcgaaggcc atccacgcac
     3421 cgtagaagat gccgataaca cccagccaca tagcaattgg cgcgaactct gccgacgcgt
     3481 tcgggaacag cggcagggag aaacgcagca aaccgtaagc ggcagttttc agcaagatcc
     3541 ccgcgaggtc aacggaaccg gcggtcggag cctgggagtg cgcatccggc agccagccat
     3601 gcagcggaac caccggcatt ttgactgcga aggcgatgaa gaagcccagc attaacaggt
     3661 attccacacc actggacatt ggcgtattca gcagctcttc atagttgaag gtccagacgc
     3721 cggtcgcatt gtagtgaaca aaaaccagcg ccaggatggc aatcaacatc accagaccac
     3781 tcgcctgggt gtaaatgaag aacttggttg ccgccgtgat acgcgtttta ccgtcagagg
     3841 ctttatgccc ccacagtgcg atcaggaagt acatcggcac cagcatcatt tcccagaaga
     3901 agaagaacag gaacatgtcg atggcaagga acacgccgat aacgccgccc aggatccaca
     3961 tcaggttgag gtggaagaag ccctgatatt tttcgatctc tttccacgaa cagagtaccg
     4021 ccagcacacc gagcagaccg gtcagcacga ccatcagcag cgacagcccg tcaatggcga
     4081 gatggataga gataccaaaa cgcgggatcc acggcatgtc gaattcagac tgccactgcg
     4141 gaattccggc ggattgcgtc agtgaataac cgccctgcaa ccacagttgc agcgacagcg
     4201 ccagcgtcaa tcccatggtg atcagcgcga tccagcgcgg caccttgacg ccaaagcgtt
     4261 cggtctgcca gcacaggaag ccgccaataa agggaattaa tattagccag ggtagtaaca
     4321 tggcgatctt tattccttgt aaaagtcccg tcaggaccgg attttcaacg aattctcacg
     4381 acagaaaatt ctcttcggac tgggggtagt gtcggatgcg ccgcgaaggc agcttatccg
     4441 acctacgttc ttatatgccc aggggcaatc ccacaatcct taactcaacg cagtaccatc
     4501 aacagtgcca gcaccacgac cgcaccgatg ctcatggatg ccacatacca gcgcagataa
     4561 ccgttctcgc ttaacagcag gcctttacct gcaaagcggg aaagaaccgc cgggatgttc
     4621 atcattgagt tcagcggatc gcgtttcagc aaccaggcaa tacccagaaa cggcttgacg
     4681 aacactttgt cgtacagcca gtcaaagccc caggcgttgt accaccaggt actcagcagg
     4741 cggcccggcg cactgttggc gatggaggtc accagagtac gtttacccag ccacagccag
     4801 gctgccagca gaatgccgac caccgcgacc acgccagaga taatttccag ggtcaacatg
     4861 ctgccgtgcg ccagttccgt cgtttgcgga agcacgccct gcagcggcgg tacaatcagt
     4921 gcgccaacga aggtggaaag gatcagcagc acaatcagcg gcaggctgtg agttacccct
     4981 ttcacggcgt gagcgtgaat ttgttctttt ccgtggaaga cgatgaaaat catacggaag
     5041 gtgtagagcg aggtcataaa cgcaccgacc aaacctgcca ccatcagatt gatatgacca
     5101 ttcgccatcg cacccgcgag gatctcatcc ttactgaaga agcccgcagt gaccagcggc
     5161 agtgccgaca gtgctgcgcc gcccaccagg aagcagagat aaaccagcgg aatagattta
     5221 cgcagaccgc ccatcttgaa gatgttctgt tcgtgatggc aggccagaat gacggaaccg
     5281 gatgccagga acagcagcgc tttaaagaac gcgtgagtca tcaagtggaa aatcgccgca
     5341 tcccatgcct gcacgccaag cgcgaggaac atgtagccaa tctggctcat ggtagagtaa
     5401 gcgagaacac gtttgatgtc ggtctgtacc agcgcggcaa aaccggccag cagcagcgta
     5461 accgccccga caatgcccac cagatgcaga atttccggcg tcatcaggaa caggccgtgg
     5521 gtacgggcga tcaggtagac acccgcggtt accatggttg cggcgtggat cagcgcggag
     5581 acaggcgtcg ggcccgccat cgcgtcggca agccatgtct gcaacggcaa ctgcgcagat
     5641 ttaccgaccg caccgcccag cagcatcagt gtcgcccaca tcagcatatt attgccatca
     5701 gcaaagtgcg ctggtgccag ttccaccatt tcgcggaagt tcagggtgcc cagttcgttg
     5761 taaagaatga acagtgcgaa agcgaggaaa acgtcaccca cacgggtcac gacgaacgct
     5821 ttcattgccg ctgcgccatt cttcggatcg gtgtaataga acccgatcag cagataggag
     5881 cacaggccca cgccttccca gccgaggtac atcagcagca ggttgtcggc aagcaccaga
     5941 accaccatgc tggcgatgaa caggttggtg taggcgaaga agcgagagta gccttcttca
     6001 ccgcgcatgt accaggaggc gtacatgtga ataaggaaac ccacaccagt gaccaccgag
     6061 agcatggtca gcgacaggcc gtccagcacc aggttaaaac cgatgttaaa gtcgcctacc
     6121 gacatccacg tccacagcgg ctggctgtat gcctgctcgc cgttagcgaa gaaatcaacg
     6181 ccgataaagg cggttaccag cgccgccagg cccacagagc caacgccgac gatcgccgaa
     6241 acgttttcag accagcgccc acgggagaac gccagcagga cgaagccaat caatggcaaa
     6301 ataatggtta aggcaagcat gttcatccgt gcatctcact tactgaatcg atgttcaggt
     6361 tctggcgacg acggtgaagc tgcagcagca gcgcaaggcc gatactcgct tctgccgccg
     6421 cgaggctgat ggcgagaatg tacatcacct gaccgtcggt ctggccccag tagcttccgg
     6481 cgaccacaaa ggccagcgcg gaggcgttaa tcatgatttc cagaccaatc agcataaaca
     6541 gcagattgcg acggataacc agaccggtta agccaagaac gaataagatt gccgcgagga
     6601 tcagtccatg ttgtaagggg atcatgcgtg ctcctccgtt tttcttttcg cgctgtcgtc
     6661 tttacgattg ctcagcactt cacccgctcg ctcttcacga ccgacgtgga aggccacaac
     6721 cagacccgcg agcagcagca tagaagccag ttccaccgcc agtacgtaag gcccgaacag
     6781 cgtaataccc actgctttag cactgattgg cgtaccgtcg ataccctgat cattaacacc
     6841 aaggatggcg taaacaatca ccaccagcat gatggccgac aaaattgccg gaccaatcca
     6901 cacctgcggt ttcagccact ggcgttcctg ttcgatttct gaaccgccca ggttgagcat
     6961 catcaccacg aacacgaaca gcaccataat ggcacccgcg tagacgataa tttccagcgc
     7021 accggcgaag taagcgccca gtgagaagaa caccccggaa atcgccagca gcgaaataat
     7081 caggtacagc agtgcgtgta ccggattggt atgggtgatc actcgcaagg tcgcaagtat
     7141 ggctatcagg ccacagatat aaaaagcgaa ctccattgcc cctctcctta cggtaacagg
     7201 ctcttgacgt cgataggctt ggcttcgttc tctgcttcgc ccttatcttt gccgtcgatt
     7261 gccatacctg ccatccggta gaagttatat tccgggtatt tgcccggacc ggagatcagc
     7321 agatcctctt tttcgtaaac cagatcctgg cgcttgtatt cccccatttc gaaatccggg
     7381 gttaactgaa tcgccgtggt cggacaggct tcttcgcaca gaccacagaa aatgcagcgt
     7441 gagaagttga tgcggaaaaa ttccggatac cagcgaccgt ctttggtttc tgctttttgc
     7501 agcgagatac agccgaccgg gcaggctacc gcgcagaggt tacaggctac gcaacgctct
     7561 tcgccgtccg ggtcgcgggt cagaacgata cgaccacgat aacggggcgg cagatagacc
     7621 ggctcttccg ggtacattcg cgtttcgcgt ttggcgaacg cgtgcaggcc gatcatccag
     7681 atactacgaa cctgggtgcc gaaacctact aacaattctt ttaaggtcat ggtcttattg
     7741 ccccttattg cgcctgccag agaatgacag ccgccgttac cagcaagttg atcagcgtca
     7801 gcggcaggca gattttccag ccgaaggaca ttacctggtc ataacgcgga cgcggtaacg
     7861 acgcacgaat caaaatgaac atcatcataa agaacgcggt tttcagcgcg aaccagatga
     7921 atggcggtaa caacgggcct tgccagccac cgaagaacag cgtcaccatc aatgcagaga
     7981 tggtcacaat cccgatgtac tcacccacga agaacagacc gaacttcata ccggaatatt
     8041 caatgtggta accatccgcc agttcctgct cggcttcggg ttggtcaaac gggtgacggt
     8101 gacacacagc cacgcccgcg atggcaaagg taataaaacc aaagaattgc gggatgacgt
     8161 tccacacatg cgcctggcta ttgacgatgt cggtcatatt gaatgaaccg gcctgcgcca
     8221 ccacgcccat cagggaaagc ccgaggaaca cttcgtagct cagggtctgc gcagaagcac
     8281 gcatcgcacc gagcagcgag tatttgttgt tacttgacca gcccgcaaac agcactgcgt
     8341 aaaccgccag accagccatc atcaggaaga acaaaatccc gatgttcagg tcggcaacca
     8401 cccaacccgg actgactggc acaatcgcaa aggccagcag cagtgaggta aaggcaatca
     8461 tcggtgccag ggtaaagatg acgcgatccg agaatttcgg gatccagtct tctttaaaga
     8521 acattttgat catgtccgca accagctgga gcgaaccgcc ccaaccaaca cggttaggtc
     8581 cgtaacggtt ctggaacaga cccagcaggc gacgttcgcc aaagctcatg aatgccccgc
     8641 aggtgacaac caccagcagg atcaccaccg ctttgaggat ggtcagcagg atctcaatca
     8701 gttccggtga tatccaactc attgttgtgc ctccttgaga tcctcaagat gcgcgccagc
     8761 cagcaccgga gcaatgccgg acatacccat cggcaagccc acctgccctg ccgtcagccc
     8821 ttcggcgatt tcaaccggca gcgtgaccgt gttgccatcg taactaaagg agacgcgtgt
     8881 acctgcgttc acacccaact tcgcggcatc cgctgggttg agtttgatgt acggctgcgg
     8941 catacggctc cggaagaccg gagcacgctg tgacaattca tcgctgccaa acaggtggta
     9001 gtacggcgcg atacgccatt tcccatcctg cggctggaag cgtgccggta cgctggtgaa
     9061 gtaatccaga ccgttttcgc tggtttcaaa cagacgcacg cccggatcgc caaagcgcag
     9121 tttgccgccc acttcgtcct ggaatttgtt ccacgcctgc ggggagttcc agcccggcgc
     9181 ccaggcaaac ggcacttgcg aacggtgcgc ggtcggctgg ttgttacctt ccatcgagaa
     9241 ggtgaacatg gtgtcaatat cctgcggctg acgcggctca tgaacgctga tattggcgcg
     9301 catggcggta cgaccgctgt aacggtgcgg ttcacgggcc agtttctgcc cacgaatacg
     9361 gaatgtcgca tccggcgcag catctttgat acctgccagt tccgggattt tcgccacaac
     9421 agcgtcaatt acatggtcga gctgcgtcca gtccacttca cggctcagca gagtgctgtg
     9481 cagcgagtgt aaccagcgcc agctttccag catgacagtt ttgctgtcgt aataagcagg
     9541 atcgtaaacc tggaagaaac gttgggcgcg gccttcgttg ttgatcaccg taccgtcgct
     9601 ttcagcaaag ctggcagcag aaagtaccag atgggcgttt tccataatcg ctgtgcgttg
     9661 atgatcaacc accatcacca gcggcgcttt agccagcgca gcattcacgc gggtagcgga
     9721 agcgtgacga tgcagatcgt tctccagcac caccaccgcg tcggcgcgtc cggtttccag
     9781 ttcggttaac gcttcttcaa gcgatccacc gcccataatg cccagcccca tgctgttgac
     9841 ggaacgggca atcatggtga taccgacgtc agcgccgcga cctttcaggg ctttcgcgac
     9901 gttagccgca gcctgaatta cttcagcgct accggcgttc gtcccggaga taatcaacgg
     9961 tttcttcgca ccagccagtg cctgcacgat gacgtcgatt ttgctttgca gctcaggttc
    10021 gataccgtca accgctggtg cagagttatc cagcgcatgg gcgatggcaa aacctaaacg
    10081 cgcctgatct tcaaccggtg cgcggtaagt ccacgccgcg atatcatcca gacgggtgtc
    10141 atcaacgttg gtaacaaaca gcggatgctt cgcacgttga ccgatgttga ggattgccgc
    10201 aatctgccag tcagccactt tctgtgctgc cgccatttcg cgcgctttac ctttcaccgc
    10261 ctgacggact gccagcgcga cgcgcgcgcc ggtctgggta acgtcttcgc ccagcaccag
    10321 taccgcatcg taagattcga tttcgcgcag agccggagta taaatgccgc cttcacgcag
    10381 cactttcagc gccagttgca gacgttcctg ctcaccgtga gcgataccgg tgtagaagtt
    10441 ttcttcgccc accagttcac gcagcgcaaa gttgctttcc acgctggcac gcggagaacc
    10501 aataccgatc actttcttcg actgacgcag aatatctgcc gcgccctgca ttgcctgttc
    10561 ggcgttgagg gtaatgaaat catcgccacg acgctgtact ggctgacgcg gacgatcttt
    10621 caggttgacg taaccgtaac cgaaacgacc acggtcgcag aggaagtagt ggtttaccgt
    10681 accgttgtaa cggttttcga tacgacgcag ttcgccgtaa cgttcaccgg ggctgatgtt
    10741 acagccgatg gaacattgct ggcagatgct cggcgcaaac tgcatatccc atttacggtt
    10801 gtaacgctcg gagtgcgttt tgtcggtaaa tacgccggtc gggcaaattt cgaccaggtt
    10861 accggaaaat tcgctttcca gcgtgccgtc ttccgggcga ccgaagtaga cgttgtcgtg
    10921 cgcgccataa acgcccagat ctgtaccgtc agcgtaatct ttgtagtaac gcacgcagcg
    10981 gtagcaggcg atgcagcggt tcatttcgtg agagatgaat ggccccaaat cctgattacg
    11041 gtgggtacgt ttggtgaaac ggtagcgacg gaagctgtgt ccggtcatca cggtcatatc
    11101 ctgaagatgg cagttaccgc cttcttcaca taccggacag tcgtgcgggt ggttggtcat
    11161 taaccactcg accacacttt cacggaactg tttcgcttct tcgtcgtcaa tggaaataaa
    11221 ggtgccatcg gaagccggtg tcatacagga catcaccaag cgaccacgcg tgtcttccgc
    11281 gttttggtat tgctttaccg cacactggcg gcaagcaccg acgcttccca gcgccggatg
    11341 ccagcaaaag taaggaatat caaggcccag agacagacaa gcttccagca ggttgtccgc
    11401 tccgttgacc tcgtattctt tgccgtctac atgaattgta gccattagca tgcttccagt
    11461 tttctcagtc agagactgag cgttaatcga aattcggtta ccagcgctct ttcagcaggt
    11521 tcggctgaat cccattaatc aaatgggtat tgctgaacgg ctgtttgatt cccgcctcaa
    11581 attcttcgcg gaaatatttg atggcgctct gtaacggctc cactgcacca ggtgcgtggg
    11641 cacagaaagt tttacccggg cctaagaatc gacacagttg ctcaagtgtt tcgatatcgc
    11701 ccggctgacc ttcaccacgc tccagcgcac gcagaatttt cacgctccac ggcagaccgt
    11761 cgcggcacgg cgtacaccag ccgcaggact cacgggcgaa aaactcttcc aggttacgca
    11821 ccagcgacac catgttgatc tcatggtcaa ccgccatcgc cagcgccgta cccagacggc
    11881 ttcccgcttt accgatactt tcgaattcca tcggcaggtc aaggtgcgct tcggtcagga
    11941 agtcggtgcc cgcgccgcct ggctgccagg ctttgaattt cagaccgtca cgcataccac
    12001 cggcgtaatc ttcgaggatc tcgcgcgcgg tagtaccaaa cggcagttcc cacaggcccg
    12061 gatttttcac ccgaccggag aagcccatca gcttggtgcc agcatcttta cttttcgaga
    12121 tgttctgata ccactccacg ccgttagcga ggatcgccgg aacgttacac agggtttcga
    12181 cgttgttgac acaggtcggt ttgccccata cgccggaggt tgccgggaag gggggtttcg
    12241 agcgtgggtt agcacgacgc ccttccaggg agttgattaa cgcggtttct tcaccgcaga
    12301 tgtagcgccc tgccccggta tggacgaaca attcgaaatc aaaacctgtt cccataatgt
    12361 ttttgccaag cagacccgct tcggtggctt cggcaatggc acggcgtaga ttaactgccg
    12421 cttcgatata ttcgccgcgc aggaagatat agccacggta agctttcagc gcaaacgcgg
    12481 agatgagcat gccttccacc agcaggtgcg gcagttgctc catcagcagg cggtctttat
    12541 aggtgcccgg ctccatttca tcggcgttac acagcaggta acggatgttc atggattcgt
    12601 ctttcggcat cagactccac ttcaggccag tcgaaaagcc cgcgccgccg cgccctttca
    12661 gaccagcgtc ttttacctga ttaacgattt cgtctggaga cagcccggtc agcgccttac
    12721 gcgcgccttc gtaaccgttt ttgctgcgat attcgtccag ccacacaggc tgtttgtcat
    12781 cgcgcagacg ccaggtcagc ggatgcgttt cgggagtacg gataatgttt ttcatttata
    12841 ccgctccagc agttcaggga tcgcttccgg ggtcagatgc gcgtgagtgt cctcatcgat
    12901 catcatattt ggccctttat cacagttccc caggcagcag gttggcagca gcgtaaagcg
    12961 accgtcaaac gtcgtttgcc ctggtttgat gttcagtttt ttctccagcg ccgcctgaat
    13021 accctggtaa ccgttgatgt gacagaccac gctgtcgcaa taacggatca catggcgacc
    13081 aaccggttgg cggaagatct ggctatagaa cgttgccacg ccttcgacat cgctcgccgg
    13141 aatacccagc acatcggcga tcgcgtggat cgcgccgtcc ggcacccagc cacgctgctt
    13201 ctgaacgatt ttcagcgctt caatggacgc cgcacgcggg tcttcgtagt ggtgcatctc
    13261 gtgctcaatc gcttcacgct ctgccgcact cagctcaaaa gcctcggttt gtggttgttg
    13321 attctcgtgc ataattagcg gtccacatct gacataacaa aatcgatact gcccagataa
    13381 acaatcaggt cagacaccag gctgccgcgg atcgccgccg gaatttgctg caaatgcgca
    13441 taactcggcg tgcggatacg agtgcgataa ctcatggtgc taccgtcgct ggtcaggtag
    13501 taactgttga tccccttggt cgcctcaatc atctggaaag attcattggc aggcatcacc
    13561 ggcccccacg acacttgcag gaagtgggtg atcagggttt cgatatgttg cagcgtgcgc
    13621 tctttcggcg gcggcgtggt cagcgggtga tccgctttga acgggccttc cggcatgttg
    13681 ttgaggcact gctcaagaat gcgcagactc tggcgcagct cttccacttt cagcattacg
    13741 cgggtgtagc agtcagaaac accaccacca accgggattt caaagtcgaa gttttcatag
    13801 ccagaataag gacgcgcctt acgcacgtcg aagtcgatcc cggtagcacg caggcccgcg
    13861 ccagtggtgc cccactccag cgcctctttc gcgccatagg cggcaacgcc ctgggaacga
    13921 cctttcagaa tggtattttg cagcgccgct ttctcgtaag acgccagacg tttcggcatc
    13981 cagtcgagga actcacgcag cagacgatcc cagccgcgcg gcaggtcgtg cgctacgccg
    14041 ccaatacgga accacgccgg gtgcatacgg aaacccgtga tcgcttccac cagatcgtaa
    14101 attttctgac gatcggtaaa ggcgaagaac acgggcgtca ttgcgccgac gtcctgaata
    14161 aaggtcgaga tatacagcag gtggctgttg atgcggaaca gttcggagag cattacgcga
    14221 atgacgttaa cgcgatccgg cacggtgatc ccggccagtt tctctaccgc cagcacgtaa
    14281 ggcatttcgt taacgcagcc gccgaggtat tcgatacggt cggtatacgg aatgtagctg
    14341 tgccaggact ggcgttcgcc cattttctcc gcaccacggt ggtggtagcc gatgtctggt
    14401 acgcagtcga caatctcttc gccatcgagc tgcaaaacga tacggaaagc accgtgcgcc
    14461 gacgggtggt tcggaccgag gttgaggaac atgaagtcct cgttttcggt gccgcgcttc
    14521 atcccccact cttccggttt gaaggtcagg gcttccatct ccagatcctg tttggctttg
    14581 gtcagctcaa acggcgagaa ttcggtagcg cgcgccgggt aatctttacg cagcgggtga
    14641 cctttccagg tttgcggcat catgatgcgg cgcaggttcg ggtgaccgtc gaaagtaatg
    14701 ccaaacagat cccaggtttc acgctcatac cagttagcgt tcgggaacag tttggtgaag
    14761 gtcggtacgt gcaggtcgtt ttctgccagc gccaccttca gcatgatgtc gcggttacga
    14821 tcgatagaaa tcagatggta gaaaacggaa aaatccgcgg caggtaaccc ttcgcggtgt
    14881 gtgcgcagac gttcgtccat gccgtgtaag tcaaacagca tgacgtaagg tttcggcagt
    14941 ttctttaaga aatcgcccac ttccagtaat tgttcacgct tgatccacac aacgggaacc
    15001 ccggtgcgag tcgcctgaac agtaaaggca tccggcccaa aacggttgcg cagttcgcca
    15061 atcaccggat catcaagatg atcgcgggtc tgccaggcgg gttcttgcgc ggttaagtcg
    15121 gtcatattgt tcaccattgc aaatggtccg tggtgactgt cggcgcagta agcttcgcga
    15181 aatagattta ggaataagcg aagaaaaatc cctttgccga caggcgcaaa ttaaatctcg
    15241 tcaggtgtac gcaggttggt tacggcaatg cgttcaccgc gcttgcgttc gcgctctgat
    15301 tgcatattgg cgcgataaac gccctgatcg ccaaccaccc aggagagcgg acgacgttct
    15361 ttgccgatag attcctgcaa cagcatcagt gcctgcatgt acgcttcagg acgcggcggg
    15421 cagcccggga tatacacatc aaccgggatg aatttatcga cgccctgcac aacggaataa
    15481 atatcgtaca taccaccaga gttggcacag gcacccattg agataaccca ttttggttcc
    15541 agcatctggt catacagacg ctgaataacc ggtgccattt tggtaaagca ggttcctgcc
    15601 actaccatca ggtcagcctg acgcggcgaa gcacgcaata cttctgcgcc aaaacgcgcc
    15661 acgtcatgca ccgcggtaaa cgaagtcacc atctcaacgt aacagcagga aagaccgaag
    15721 ttatacggcc aaattgagtt tttacgaccc cagttaacca tgtcattgag cttgcccata
    15781 aacacgtttt tgttaacttc ttgctccaga gggtcggtta cgatctcctg cttttgcagg
    15841 gggtaacggt cgttctcacc gttgggatct atgcgggtga gcgtataatc catcttaatg
    15901 cctcgcggtt agcgttgacg attagcgata ctgttcgttt ccgggttcat acgctcgcgg
    15961 cgtgaacgcg cgggcgtcca gtccagcgcg ccaatacgca ccagataaac cagacctgcc
    16021 agtaacacaa aaataaaaat tgcagcttcc acaaagccta cccagccgct ttcgcggata
    16081 gaggttgacc atgcgaacag atacagcgct tcaacgtcga agataacgaa gaacatggcc
    16141 accagataaa acttggcgga caggcgtaag cgggcggagc cgaccgagtc gataccggat
    16201 tcaaacggca cgtttttcga cctcgcgcgt gcgcgaccgc ctaaaaacca accgcctacc
    16261 agcatcaggc aacacaggcc aatggcaacg ataagaaaga tagcgaatgc ccagtgatga
    16321 gcgatgactt cagtggatgt tgacatactc attgcttact catcaaaagt agcgccagat
    16381 tcactgctct tcacggcaga tggacgccac atcgattcat ggggaggaat aaaaaaaacc
    16441 ttacaatcac tgtagaaatt cttttataca gctaattgat gtggtctttt actcctttct
    16501 ataacctttt gtcaacttta acaaaagttt cttcacatta gtttacataa tatcaacacc
    16561 attagcattt aatgcccttt caccccagat ccttgacgac tccaggataa ttagatgttg
    16621 ttgaatcgtg tccgttgtga agcaatggaa aaaatacggg tctattttga caggaatttg
    16681 tgtcgattcc tccccccaaa agagagtatt ttcttgatct gtgacacgct tttgtcattc
    16741 cataacaaaa acgcagcaac aaatttacgt attttttaac atcattgtag caggtgattt
    16801 ttttcaggcg attatttgtg cgtacggggc gtgaatctct ggtgattgaa aaatgaacag
    16861 ttttgtacgt tctgcactat gcgaggaagg atttttacta aaaaaaagcc gctggggtct
    16921 aaaacacccc cagcggctcg ttttttacac tattgtctca ggaattatct atcgtccgtc
    16981 gattactcga tatccctttc aatcaacaat gaatcatccc cttccggagc agacattggg
    17041 ctgtattgcc acggattatg gtagctctcc atcgcctgat agatcacctg ggccagctca
    17101 ttattactgg acgggtcata gcacagcaaa tattcggtat caggtagcgg cggtaagcca
    17161 tctactccac tcagcacacg caagtccgga ctcatcatct cgaccggcct tgccgtcacg
    17221 ccaagaccgg cttttactgc cgcacgaact gccggaagcg tcgaggcgac ataagccagt
    17281 cgccatggaa tatctgcttt attaagcgtc gccagcacca tatcgcgaaa cggacttgga
    17341 tcatccagca atacaagagg gatcggctca cctttttgca atacgtattc cgctgcgcag
    17401 taccagtgtg ttggcgaggt acgcaggttc aatgccttaa acgctgaagg acgatgggtg
    17461 gttaccatca aatccacttc ctgggattcc agcatttctg ccatataggc attacgttta
    17521 acgcggacat ccagcgctaa tttcggataa accgaactca cgcgatttaa caggaaaggt
    17581 aagatcgtat cggcagattc atctgaagca ccgatagtta acacgccctg aagattactg
    17641 aacattaatg atgagcaggc ctcatcatta aaacgcagga ttttcctggc gtaaccaaga
    17701 agttgaatgc catgttcagt taacagtttg ttgcgaccgt gacgagcgaa cagttctttc
    17761 ccaacgagtt gttccagacg ctgcatttgc tgacttacgg cggactgagt acgacacaca
    17821 gcggcagctg cggcagcaaa agtgttcaga tcggcaacag caacaaatgt tctcagcaga
    17881 tcgaggtcga ggttaattat cggacgattt gcacttatca tatattatca cttactggcg
    17941 gctcatactg agctggttaa tgctgtgcac acacaaacaa gcaattccat ttgtaatgtg
    18001 cctccctggc agtttcatcc ggaacccgga cgaaagtaaa aatgcatatg agttgcacta
    18061 aaaaagcgac tcacattgtt ccgttataat gcctgaagta gatcacagaa tatatcttca
    18121 gggatcgcat atctattaag ttactcactc ttttctattt atgacatgcg cgtgtttgta
    18181 taaatgtaaa tgtgagtcct tgttccactc tcgtgcagca tcgctggtca tacgcgaaca
    18241 cgtacaaaca gcaatggtga ggcggcatca agagcaggga tccgtcattt atgcgagcat
    18301 tttaccccaa aaacctttta tttataaggg tcattgcgaa ttatctgatg caaagttatg
    18361 ttatgttagg caaagtaatc ttctattatt aataagcaca tcaaaacctt tttgaatatt
    18421 aaataataat taattagcat catcctcatt cattaattcc gcttaacaat agtttcacac
    18481 cttgccccca ttgcgacact tcggaaatca tcacgctgtt aaactctccg taatgcggaa
    18541 atatcatcat cgcgtttcat tagtgaattc ttaaatgagg cattttcaca caattatctt
    18601 acagataaaa aaaccagact tacaattaag aatcagaaca attcactata taacattgca
    18661 tataaagcat atacacctca ttattttgtc attattaagt tattaacagc acaatcgagc
    18721 cttcccctct ggcaaaatct tattctgcag accttcaaaa caccgtcctg ggggagtaca
    18781 ttgttctaag ctgacttcca cggcagggag tggcgataac agcaaaaaag gtcaagattc
    18841 atgtccccca ttgaaaaatc cagcaaatta gagaatgtct gttatgacat ccgtggtccg
    18901 gtgctgaaag aagcaaaacg cctggaagaa gaaggtaaca aggtactgaa actgaacatc
    18961 ggcaacccag ccccgttcgg ttttgacgcg ccagatgaaa tcctcgttga cgtgatacgc
    19021 aacctgccta ccgctcaagg gtattgcgat tccaaaggtc tttactccgc gcgtaaagcc
    19081 atcatgcagc actaccaggc tcgtggcatg cgtgatgtta ccgtggaaga tatttacatc
    19141 ggcaatggtg tatcggagct tatcgttcag gcaatgcagg cattgctgaa cagcggggac
    19201 gaaatgttgg ttcctgcacc agattaccca ctatggaccg cggcggtttc gctttccagc
    19261 ggtaaagcgg tgcattatct ttgcgatgaa tcctctgact ggttcccgga cctcgatgat
    19321 attcgcgcta aaattacgcc tcgtacgcgt gggattgtta ttatcaaccc aaataaccca
    19381 accggcgcgg tatattccaa agagctttta atggagattg tggagattgc acgtcagcat
    19441 aatctcatta tcttcgccga tgaaatttat gacaaaattc tctacgacga tgctgagcat
    19501 cactcaattg cgccactggc acctgacctg ctgaccatta cctttaacgg actgtcgaaa
    19561 acgtaccgcg ttgcaggctt ccgtcagggg tggatggtgt tgaacgggcc gaaaaaacac
    19621 gccaaaggct acatcgaagg tctggaaatg ctggcttcaa tgcgcctgtg tgctaacgtt
    19681 cctgcgcaac acgccattca gaccgcgctg ggtggttatc agagcatcag tgaatttatt
    19741 acccctggcg gtcgtcttta tgagcagcgt aaccgcgcgt gggaactgat caacgatatt
    19801 ccgggcgttt cctgcgtgaa acctcgtggt gcgctgtata tgttcccgaa aatcgacgcc
    19861 aaacgcttta acattcacga cgatcagaaa atggtgctgg atttcctgtt gcaggaaaaa
    19921 gttctgttgg tgcaagggac ggcattcaac tggccgtggc cggatcactt ccgcattgtc
    19981 acgctaccgc gtgtcgatga tatcgagctg tctttgagca agttcgcgcg tttcctttct
    20041 ggttatcatc agctgtaatc ttaatttcac tgccggagat tgcatccggc agtgttatcc
    20101 cgccacaatg acctgatgat gtcatcatac gtaaggtcac tatgaaacag agccattttt
    20161 ttgcccatct ctcccgcctg aaactcatta accgctggcc gctcatgcgc aacgtgcgga
    20221 cggaaaatgt ttccgaacac agtttgcagg tagcgatggt cgcccatgcg ctggcagcta
    20281 tcaaaaatcg gaaatttggc ggtaatgtca acgccgaacg tatcgcttta ctggcgatgt
    20341 accacgatgc ctctgaagtg ctcaccggcg atctccctac tccggtgaaa tacttcaatt
    20401 cgcaaatcgc tcaggagtac aaggctattg aaaaaatcgc tcagcaaaaa ctggtcgata
    20461 tggttccgga agagctgcag gatatctttg cgccgttaat tgacgagcat gcatatagcg
    20521 atgaagaaaa atcgctggtg aaacaggcag atgcactgtg tgcatatctg aaatgtctgg
    20581 aagaactcgc ggccggaaat aatgaattct tgctggcaaa aacgcgactg gaagcgacgc
    20641 ttgaagcgcg tcgcagccag gagatggact acttcatgga agtatttgtt cccagcttcc
    20701 atctttcgct cgatgagatt agccaggatt caccgctata agcagccgga gtctgcgtcg
    20761 catcaggcaa taagcgccgg atgcgacatc aggctcttgt caaaacggaa acagcatcgg
    20821 gatcatcacc acacaaaccg ccatcacgat aatggtgaac ggtaccccca acttcacaaa
    20881 gtcactgaag ctgtaatttc ccggacctaa aaccagtgtg ttaacaggtg aagaaaccgg
    20941 tgtcataaag gcggcggatg ctgccatcgc cacgaccatc gcgaatggat aaggcgacac
    21001 acccatcgtt ttggcagcag ccagcgcaat cggagccatc aacaccgccg tcgcggtatt
    21061 agagataaac agcccaataa ccgccgacaa gacaaacaaa caacccagca tcatatgtgg
    21121 cccataaccg ccgccaatgt ccatcagccc tttcaccgcc agcgcgacac ctcccgtttt
    21181 ctgtaatgcc acagcaaacg gcatcatccc aacgatcaaa ataatgctcg gccagtgaat
    21241 ggatttatag gcgctttcgg catcgataca gcggaatttc cccatcagca agcaggcgat
    21301 gatagcggca acaggattag gaatttcatc ggtcagcatt aacgccacca tcaacaccag
    21361 acagaaaatg gcatggggtg cctgactgtg tgcgggcgat gcttcactca cttcttctgg
    21421 taagttcagc gccacaaagt cacgcccctg ttttgccagc ataccgatca gtttccagtt
    21481 accaacaacc aggatgatat cgcccagcag cagaggctca tccgccagcg aaccttccag
    21541 cgccacgcca ttgcgcttta gccccaccac attcagtccg tagcgggtac gaaaaccaat
    21601 ttcgcgcacc gatttaccaa tcagttctga ctcaggaatt aatgaaatct ctgccatgcc
    21661 cacatcaagg gcctggtcag aaaaatactc gccgcgcagt accatcggct ccagcaattg
    21721 ctcactacaa aattgccgga gatcgacatc agccgcagac atatcaataa gcaaaacgtc
    21781 acgcgcgcga aattcagaaa ccccattaac gttcacgata acgcgacgaa aacgccgcca
    21841 gcgttcaaca ccgatgacgt tagcgccata acgctcacgt aatttgagat cgtccagccg
    21901 ttgaccaatc attggcgatc cggggcgaat agccagacgt cgcgcacgcc cggtcagtcg
    21961 atattcacgg ataagatcac gaaaggttcg acgcgtccag ccttcgcgct gcggggtctg
    22021 ggtatcccct ttcagcatga aacgcatcac taacatatac aaaataccca gcaccagcac
    22081 gaccaggcca ataggtgtta cgctaaagaa actgaagccg tgatagcctt cacgcagcaa
    22141 ttcactgttg actaccaggt tcggcggcgt cgccaccagc gtcatcatgc cgctaatcag
    22201 cccggcaaaa ctcaacggca tcatcaggcg cgacggcgac gtttgcatac gcatggcaac
    22261 gcttaacacc acgggaataa agatagcgac aacgccggtt gaactcataa acgcgccaag
    22321 ccccgcgacg gtcagcatca acaaaaccaa cattttgatt tcactgttgc ccgcaacttt
    22381 gaccagccat gttcccatta cggtggcaac accggtacgg accaaaccat cgccaataat
    22441 aaacaaggcg gcaatcagga caacgttagg atcagaaaag ccggaaaata cttcggggac
    22501 cgtcagcgtt ccgcttaatg caaacgcgac aataacaaac aaagcgaccg catccatacg
    22561 cactctgccc gtcgcaaaca agacgatggc aacggccagt aatgaaagaa cccaaatcaa
    22621 ttcaccgttc acaacttatc cttgttaatt gagggggatg acttgattct gccataaaaa
    22681 agccccgacg agacggggct aaatcatgat caggtgtttc actgaataat aacatcgcca
    22741 tttggctgtt tggtcacagt aatttgctcc agactgtgga ggaccaaatc gacctcattc
    22801 aggcgcgggg tatctgccgg agcgttaacc gcaatgacat ggcaacccgc cgccaggcca
    22861 gaaagcacgc cagcgggagc atcttccacc accacacact cctgcggcgc aagccccagc
    22921 agctgcgcgc ctaacagata cgcatcaggt tctggttttc cgcgcttcac tcgctcagcg
    22981 gttacaaaca cctctggtgc gggaagccca gctattttat ggcgcgctcg cgctaccggc
    23041 atggagccag aagtcacaat ggcccacgga atacctgctt tattcaaatg actgagtaag
    23101 gcgattgccc ccggaagcgc ggtaatacct tcggtttccg tggcctcgat ttgctccaga
    23161 cgcgtaaact cggcggcaat atcagcctcg gatttgcccg ccataaaatg gcgcagagag
    23221 gtgatcgcct gtttaccgtg aatgaaagcc agcacctctt ccggcgctaa cccatgacgt
    23281 ctggcccagt tgctccacgc ccgttctacc gcaggcaggg aatccaccag cgttccatca
    23341 agatcaaaca gaaaaccttt gcaccgcacg cgggcctcct caggcgttga taatttgatt
    23401 tatttcgttg gcgctcaaat ggtactgacg cgggcaggca tgccacacat taagcatgcg
    23461 ctgatatttt tcccacattg gcgtctgggc gttaaaaccg tgagttccgg cgtcaaaatg
    23521 ggtatagcgc ccttccacat taaccataaa gcggacataa ccgaggtaac gtgcttcagt
    23581 ggcggcgtca aagccgagga aggtgacacg acgttcatcg atggattgct ggtcctgcaa
    23641 attagaccag gaaacatgca acgcatgata catctccata atgtcgatga tagtgcggca
    23701 ggtttcttct ttcagctcgc caaactcgcg atccagttca cgcatctgta atccataacc
    23761 acgctcaatg attgtttgca ggcgacggta acgttcagca tttgccggat cgagcatagt
    23821 catcatcttg tactggttag acaaaataag acgttgcgcg ttggtcattt ccattgttga
    23881 ctcctgtatc actctactac ggtgaaaaaa aagaaggctg agtatgcctt cttttatatg
    23941 cgtaatcagg ggtcaattac aaatcatcaa ggaaagtttt atccagttgt ttgaaggcgc
    24001 gcttaagcgt gtcagctaat gcctggtaat caggcttgcc ttcaacgggt gccaacacct
    24061 gtccagactc ctgcaattta ccgcgaactt cataaaacca gttaaggatt gcagggggta
    24121 atggcgttac agaacgcttg cccagccacc acaatccctg catgggtaaa cttaaggcga
    24181 acagggcagt ggcaactgcc ggcccaagct gaccgcccag ggcaatctgc cagcagagag
    24241 taaatacagc gatcggcggc ataaaacgga tcgcataacg cgtcatcttg ataacgcgat
    24301 tttcgacaaa gaccggggca aggcgttttt ccagcggcca cgtctttgag taatgctgtc
    24361 cccggcgaaa caagctaaaa aaattaacag aacgattatc cggcgttgac atgcttcacc
    24421 tcaacttcac atataaagat tcaaaaattt gtgcaaattc acaactcagc gggacaacgt
    24481 tcaaaacatt ttgtcttcca tacccactat caggtatcct ttagcagcct gaaggcctaa
    24541 gtagtacata ttcattgagt cgtcaaattc atatacatta tgccattggc tgaaaattac
    24601 gcaaaatggc atagactcaa gatatttctt ccatcatgca aaaaaaattt gcagtgcatg
    24661 atgttaatca taaatgtcgg tgtcatcatg cgctacgctc tatggctccc tgacgttttt
    24721 ttagccacgt atcaattata ggtacttcca tgtcgagtaa gttagtactg gttctgaact
    24781 gcggtagttc ttcactgaaa tttgccatca tcgatgcagt aaatggtgaa gagtaccttt
    24841 ctggtttagc cgaatgtttc cacctgcccg aagcacgtat caaatggaaa atggacggca
    24901 ataaacagga agcggcttta ggtgcaggcg ccgctcacag cgaagcgctc aactttatcg
    24961 ttaatactat tctggcacaa aaaccagaac tgtctgcgca gctgactgct atcggtcacc
    25021 gtatcgtaca cggcggcgaa aagtatacca gctccgtagt gatcgatgag tctgttattc
    25081 agggtatcaa agatgcagct tcttttgcac cgctgcacaa cccggctcac ctgatcggta
    25141 tcgaagaagc tctgaaatct ttcccacagc tgaaagacaa aaacgttgct gtatttgaca
    25201 ccgcgttcca ccagactatg ccggaagagt cttacctcta cgccctgccg tacaacctgt
    25261 acaaagagca cggcatccgt cgttacggcg cgcacggcac cagccacttc tatgtaaccc
    25321 aggaagcggc aaaaatgctg aacaaaccgg tagaagaact gaacatcatc acctgccacc
    25381 tgggcaacgg tggttccgtt tctgctatcc gcaacggtaa atgcgttgac acctctatgg
    25441 gcctgacccc gctggaaggt ctggtcatgg gtacccgttc tggtgatatc gatccggcga
    25501 tcatcttcca cctgcacgac accctgggca tgagcgttga cgcaatcaac aaactgctga
    25561 ccaaagagtc tggcctgctg ggtctgaccg aagtgaccag cgactgccgc tatgttgaag
    25621 acaactacgc aacgaaagaa gacgcgaagc gcgcaatgga cgtttactgc caccgcctgg
    25681 cgaaatacat cggtgcctac actgcgctga tggatggtcg tctggacgct gttgtattca
    25741 ccggtggtat cggtgaaaat gccgcgatgg ttcgtgaact gtctctgggc aaactgggcg
    25801 tgctgggctt tgaagttgat catgaacgca acctggctgc acgtttcggc aaatctggtt
    25861 tcatcaacaa agaaggtacc cgtcctgcgg tggttatccc aaccaacgaa gaactggtta
    25921 tcgcgcaaga cgcgagccgc ctgactgcct gatttcacac cgccagctca gctggcggtg
    25981 ctgttttgta acccgccaaa tcggcggtaa cgaaagagga taaaccgtgt cccgtattat
    26041 tatgctgatc cctaccggaa ccagcgtcgg tctgaccagc gtcagccttg gcgtgatccg
    26101 tgcaatggaa cgcaaaggcg ttcgtctgag cgttttcaaa cctatcgctc agccgcgtac
    26161 cggtggcgat gcgcccgatc agactacgac tatcgtgcgt gcgaactctt ccaccacgac
    26221 ggccgctgaa ccgctgaaaa tgagctacgt tgaaggtctg ctttccagca atcagaaaga
    26281 tgtgctgatg gaagagatcg tcgcaaacta ccacgctaac accaaagacg ctgaagtcgt
    26341 tctggttgaa ggtctggtcc cgacacgtaa gcaccagttt gcccagtctc tgaactacga
    26401 aatcgctaaa acgctgaatg cggaaatcgt cttcgttatg tctcagggca ctgacacccc
    26461 ggaacagctg aaagagcgta tcgaactgac ccgcaacagc ttcggcggtg ccaaaaacac
    26521 caacatcacc ggcgttatcg ttaacaaact gaacgcaccg gttgatgaac aggggcgtac
    26581 tcgcccggat ctgtccgaga ttttcgacga ctcttccaaa gctaaagtaa acaatgttga
    26641 tccggcgaag ctgcaagaat ccagcccgct gccggttctc ggcgctgtgc cgtggagctt
    26701 tgatctgatc gcgactcgtg cgatcgatat ggctcgccac ctgaatgcga ccatcatcaa
    26761 cgaaggcgac atcaatactc gccgcgttaa atccgtcact ttctgcgcac gcagcattcc
    26821 gcacatgctg gagcacttcc gtgccggttc tctgctggtg acttccgcag accgtcctga
    26881 cgtgctggtg gccgcgtgcc tggcagccat gaacggcgta gaaatcggtg ccctgctgct
    26941 gactggcggc tacgaaatgg acgcgcgcat ttctaaactg tgcgaacgtg ctttcgctac
    27001 cggcctgccg gtatttatgg tgaacaccaa cacctggcag acctctctga gcctgcagag
    27061 cttcaacctg gaagttccgg ttgacgatca cgagcgtatc gagaaagttc aggaatacgt
    27121 tgctaactac atcaacgctg actggatcga atctctgact gccacttctg agcgcagccg
    27181 tcgtctgtct ccgccagcgt tccgttatca gctgactgaa cttgcgcgca aagcgggtaa
    27241 acgtatcgtt ctgccagaag gtgacgaacc gcgtaccgtt aaagcagccg ctatctgtgc
    27301 tgaacgtggt atcgcaactt gcgtactgct gggtaatccg gcagagatca accgtgttgc
    27361 agcgtctcag ggtgtagaac taggtgcagg cattgaaatc gttgatccag aagtggttcg
    27421 cgaaagctat gttgctcgtc tggtcgaact gcgtaagaac aaaggcatga ccgaaaccgt
    27481 tgcccgcgaa cagctggaag acaacgtggt gctcggtacg ctgatgctgg aacaggatga
    27541 agttgatggt ctggtttccg gtgctgttca cactaccgca aacaccatcc gtccgccgct
    27601 gcagctgatc aaaactgcac cgggcagctc cctggtatct tccgtgttct tcatgctgct
    27661 gccggaacag gtttacgttt acggtgactg tgcgatcaac ccggatccga ccgcagaaca
    27721 gctggcagaa atcgcgattc agtccgctga ttccgctgcg gccttcggta tcgaaccgcg
    27781 cgttgctatg ctctcctact ccaccggtac ttctggtgca ggtagcgacg tagaaaaagt
    27841 tcgcgaagca actcgtctgg cgcaggaaaa acgtcctgac ctgatgatcg acggtccgct
    27901 gcagtacgac gctgcggtaa tggctgacgt tgcgaaatcc aaagcgccga actctccggt
    27961 tgcaggtcgc gctaccgtgt tcatcttccc ggatctgaac accggtaaca ccacctacaa
    28021 agcggtacag cgttctgccg acctgatctc catcgggccg atgctgcagg gtatgcgcaa
    28081 gccggttaac gacctgtccc gtggcgcact ggttgacgat atcgtctaca ccatcgcgct
    28141 gactgcgatt cagtctgcac agcagcagta acctcgtcat catccgcagc tttgcgctgc
    28201 ggatatctga accggaaata gtcactattt ccggtttttt attctcttaa tttgcattaa
    28261 tcctttctga ttatcttgct taactgcgct gcatcaatga attgcgccat ctcactttgc
    28321 atacttacca ctttgttttg tgcaagggaa tatttgcgct atgtccgcaa tcactgaatc
    28381 caaaccaaca agaagatggg caatgcccga tacgttggtg attatctttt ttgttgctat
    28441 tttaaccagc cttgccacct gggtagttcc ggtggggatg tttgacagtc aggaagtgca
    28501 gtatcaggtt gatggtcaaa caaaaacacg caaagtcgta gatccacact catttcgcat
    28561 tctgactaac gaagcaggcg aacctgagta tcaccgcgta cagctgttca cgacgggcga
    28621 tgaacgcccg ggcctgatga acttcccgtt tgaagggtta acctcaggat cgaaatacgg
    28681 gacagccgtt ggcatcatca tgtttatgct ggtgattggc ggcgcgtttg gcattgtgat
    28741 gcgtacagga accattgata acggtatcct ggcgcttatt cgccataccc gcgggaatga
    28801 aattctcttt attcctgcgc tgtttattct gttttcactt ggcggcgcgg tatttggtat
    28861 gggggaagag gccgtcgcct ttgccattat catcgcaccg ctaatggtcc ggctgggcta
    28921 tgacagtatt accaccgtcc tggtgaccta tattgccacg caaatcggtt ttgccagttc
    28981 gtggatgaac ccgttttgtg tggtcgttgc tcaggggatt gccggcgttc cggtgctttc
    29041 tggctccggg ttgcgcatcg tggtgtgggt tatcgccact ctgattggcc tgatctttac
    29101 catggtgtac gcctcacgag tgaaaaagaa tcctcttctg tcacgcgtgc atgagtccga
    29161 ccgcttcttt cgtgaaaagc aggctgatgt tgaacaacgt ccgtttacct ttggtgactg
    29221 gctggtattg attgtcctga ccgccgtaat ggtctgggtg atttggggcg tgatcgttaa
    29281 tgcctggttt attccagaaa ttgccagcca gttcttcacc atgggtctgg tgattggcat
    29341 catcggcgtt gttttccgcc ttaacggcat gacggttaat accatggctt catcctttac
    29401 cgaaggggcg cgaatgatga tcgcccctgc cctgctggtg ggtttcgcca aagggatttt
    29461 gctgctggtc ggtaatggtg aagcgggtga tgccagcgtg ttaaatacca tcctcaacag
    29521 cattgccaat gccattagcg gtctggacaa tgcggtcgcg gcctggttta tgttgctctt
    29581 ccaggcagta tttaatttct tcgtgacgtc cggttctggt caggcggcgt taaccatgcc
    29641 gttactggca ccgcttggcg atctggtcgg tgttaaccgt caggttaccg tgctggcgtt
    29701 ccagtttggt gatggcttca gccacatcat ttacccaacc tcagcttcgt taatggcgac
    29761 gctcggtgtg tgccgggtgg acttccgtaa ctggctgaag gtgggcgcga ccctgcttgg
    29821 actgctgttt attatgtcca gcgtcgtagt gatcggcgct cagttgatgg gctaccacta
    29881 aaaatgttaa gagccgcaat tgcggctctt ttttcattct gccgtttcag tctctacggc
    29941 ttcatttttg gcattgcgct tcatccacaa cgccagcgct ttcagcgagt ctggagtgaa
    30001 ctcatcgcag cgcgcggtga tttcttccgg cgtcagccag caaacttcac tgacttcatc
    30061 ttcctgtagg gcgaagggac cgtgagagac gcaactgaac aacgcgcccc agacacggca
    30121 atttttatct tcgaaataga actgcccgtg ctcggcaaag gggacaccgg caatgcccaa
    30181 ctcttcttcc gcttcgcgac gcgcggattc cagcagttgc tcatcggcct gaaccacacc
    30241 gcctgcggtc gcatctaaca tgccgggtaa aaagtctttt gtctcggtac gacgctggac
    30301 cagaattttg cccatgccat catgcacgac gatgtaagtt gcacgatgac gcagacactg
    30361 tgcccgcatt tgttcccggc tggcttgtgc aatgacttcg ttctcttcat tgacaatatc
    30421 cacccattca gtacttgcca aacgacgctg ttccattatc aggaaacctt ctttttctgg
    30481 cgctcttacg gcgcattttg agttgtgggt aaattacgga ttaatcgcga cctgcgcaat
    30541 gatactttga tcattgagtg cgattacact aagtacgtca ttatccagca tgccataact
    30601 cgccggatta ccgcctttcg gaatactcac cgagccgggg ttgaagtgga aaatctcccc
    30661 ccgctgttct gccactggca gatgggtatg accgtacacc agcacatcgt tctggtttaa
    30721 agcaggcaga ttttccgggc caaaaagatg accatgcgtc aaaaacagac gttgtttttc
    30781 cagtaatacc tgttgccacg gcgcggttat cgggaaatgc agcagcattt gatccacttc
    30841 gctgtcgcag ttgccgcgca cagcgataac cttatgcgcc acttcattaa gcagttcagc
    30901 gactttggct ggcgcgtaac cctccggtaa agcattacgc gggccgtgat tcaacacgtc
    30961 gccaaggatc accagccact gggcaccgct ttgggcaaac aactccagaa cacgttccgt
    31021 cgccggtaac gacccatgaa tgtccgatgc aaacatcagt ttcatcactc actcctcgtc
    31081 gaaaaaacgt aagctatgat actggattct gtcgttgcta tcagccagat tgcttcgccg
    31141 aaagtgcaat aaaacgctgg acagacgctc gctgccactg gaatgtcgca taatccacca
    31201 ggcgttctgg cacctcatcg ccatgtagta ccaggcggtt aatcattagc gccagatcag
    31261 tatcagcaat gcaccattca ccaaataaat tcggctgacc cagtgctaac aaatgttctg
    31321 ccatcgcgaa cagtttctct gcactggctt ttccctcggc cgttagtggc gcttttttcg
    31381 cccccgcaaa gacaacatcc gtcggacgct cttcgcggat gggcatcaga tcgctgcgca
    31441 gccaggcctg aatctgtcgt gcacgcgcac gattttctaa atcaagcgga taaatacgtt
    31501 cccaggtcgg tggcgcaaat cgatcttcca gatactccgc aatggcagaa gattcactca
    31561 actcaaaatc atcgatttgt aataacggca cacggcgtgt ctgaccgtaa ccttgccacg
    31621 tcggctgcaa atgttcaccg ctgtcgaggt cgatggtctt gatatgaaac gacaggcctt
    31681 tttcctgcaa cgccacccag gcagataaca cataagggga gaaaaagtgg gcatctgacc
    31741 aaagcgtgat agcgggttta ctcattatgt cctcggcaat aaagggggct ggctatcaaa
    31801 ctatagagcc tttttgtcac tgtcacgtga cgaatactca cgtaagtcgc cgcgctttct
    31861 atacttgtgg tgttcagatg attaaacaac cggagctgca atgatcgatc tctatttcgc
    31921 cccgacaccc aatggtcaca aaattacgct atttctcgaa gaagcagagc ttgattatcg
    31981 cttgattaag gtagacctgg ggaaaggcgg tcagtttcgt ccggaatttt tgcgcatttc
    32041 gcccaacaac aaaattccgg caattgttga tcattctcct gccgatggcg gcgaaccgct
    32101 aagcctcttt gagtctggtg ccattttgtt gtatctggct gagaaaacag gactcttttt
    32161 gagtcatgaa acgcgtgagc gcgccgccac attacagtgg ttattctggc aggtaggcgg
    32221 actggggccg atgcttgggc aaaatcatca ttttaatcac gcagcccccc aaaccattcc
    32281 ttacgctatt gaacgttatc aggttgaaac tcagcgtctt taccatgtac taaacaagcg
    32341 gctggaaaac tcgccctggc tgggaggcga gaactacagc attgcggata ttgcctgctg
    32401 gccgtgggtt aatgcctgga ctcgccagcg aattgacctc gcaatgtatc cggcagtcaa
    32461 gaactggcat gagcggatcc gttcgcgccc tgccaccggg caggcactgc taaaagcaca
    32521 actcggtgat gagcgttcgg atagttaaca gaaacaggtt ctcgtgtatt atttcatcct
    32581 aagtaaaaca acggagaacc tgcaatggca caacctgccg ctattattcg tataaagaac
    32641 cttcgtttac gcacgtttat tggaattaag gacgaagaaa ttaacaaccg tcaggatatt
    32701 gttatcaatg tgacgatcca ctaccccgcc gataaagcgc gcaccagcga agatattaat
    32761 gacgcgctga actatcgcac cgtcaccaaa aacattattc agcatgtaga gaataaccgt
    32821 ttctctttgc tggaaaaatt aactcaggat gtgctcgata tcgcacgtga acatcactgg
    32881 gtgacttatg ctgaagtgga gatcgataaa ctacacgcgc tgcgctacgc cgattcggta
    32941 tccatgacct tgagctggca gcgttaaccg ccatatcggg aggctgcatg aacatagtga
    33001 tcaccggagg gacgggatta attggtcgcc atttgattcc gcgtttgctg gagctgggcc
    33061 atcaaatcac ggtagtgacg cgtaacccac agaaagccag ttccgttctc ggccctcggg
    33121 tgacgctatg gcaagggcta gccgatcaaa gcaacctcaa cggcgttgat gcggtaatca
    33181 acctggccgg agaaccgatt gctgataaac gctggactca cgagcaaaaa gagcgtctct
    33241 gccaaagccg ctggaatatc acgcaaaaac tggtcgattt gattaatgcc agcgacacgc
    33301 caccgtcggt actcatttcc ggctcagcaa cgggctatta tggcgactta ggtgaagtgg
    33361 tggttaccga agaggaaccg ccgcataacg aatttaccca taaactctgc gcccgctggg
    33421 aagaaattgc ctgccgggcg caaagtgaca aaacgcgagt gtgcctgctg cgtaccggcg
    33481 tagtgctggc accggatggc ggtattctcg gtaaaatgct gccgccgttt cgtcttggcc
    33541 tgggcgggcc gattggttcc ggtcggcagt atctggcctg gattcatatc gatgatatgg
    33601 tcaacggcat tctctggctg ctggataacg agctgcgcgg gccatttaat atggtttcgc
    33661 cctacccgat acgcaatgaa caatttgccc atgcgctcgg tcatgcgctg catcgcccgg
    33721 ctattttgcg cgtccccgca accgccattc ggctgttaat gggcgaatct tcagtactgg
    33781 tattaggtgg acaacgcgcg ctgcctaaac ggctggaaga agcgggtttt gcgtttcgtt
    33841 ggtacgattt agaagaggcg ctggcggatg tcgttcgctg atgtggttta ctgcaaacat
    33901 ccgccagtta actcccggtg ttacagggtt agtggctttg cgcgataaga tcgtctggtg
    33961 aaagtcgggt caccatcata actaactctc ggtctaaacc tctatccagc atctcctgag
    34021 caatacgcag ggcttcttcg cgtttgccaa gaattaatcc atcattccgc ccttcttcac
    34081 gtaatctgtc agcaatggtc atcagtttct ccttttcctg tggtgcgcgt tctgctatct
    34141 caccaataaa tgcacggaaa cgctgggcat ctcccgtttg taatacataa ttaaacaggg
    34201 cttttagctg tctgtcatta gtgttccctg taactagcag cgaaacaatt tggtcgacta
    34261 atcccaacag atcgcgctga cgaatatgtt tctgaattaa ctccaacagc gccattttgc
    34321 gatgttgcat aatctcgtca tccggcacca cggtaatatc caccaacgga aaagccgatg
    34381 aatatatttt acgggcaatc gcaggctcgg caaattcatc aagccagcag agtgaataag
    34441 gataaggact tctgcaacca tgataaaaca gcattgggat caccaatgga agctctttat
    34501 agcctgcatc cagatggttt tgcatcgccg cgatggaata gcgcatcatg cgaaaagcca
    34561 ttaattcttc cggcttactt tggtgctcta tcactacata aatataaccc actccctcct
    34621 gcgttttcac agaccacaag aggtcggaat aatattgccg caggtcttca tcaataaaac
    34681 tgtttggttc cagtttaagc gtcgttaaat cacacagttt gcgcagcggc gcgggaagat
    34741 gaatatcaat aaaatcccgc gcggtgtctg gatggcgtaa aaaagattta aataccgcat
    34801 catgcggcgt ggaagttgtt gatattgtca tggcgttccg tcacccctca aatttaagat
    34861 gacgcgacaa taaccacgat taacacccaa tgcagcaggc agtttttatc tttggacagc
    34921 gaattcagaa gtatttttag cggactgcaa cttttacatt tcagcggaag ctgactcgca
    34981 aattccccat gaaaatactc cggcagcgtt agccaccgga gaggaaatta tttcagcgat
    35041 cccttaagga actgttgcag acgagggctt tgcgggttgc caaataactg ctccggcgcg
    35101 ccctcttctt ctattttccc ctgatggagg aaaatgacgt gagtagaaac atggcgagca
    35161 aagcccattt cgtgagtcac taccaccatg gttttcccct cttctgccag ttgctgcata
    35221 atacgcaaca cttcgcctac cagttcagga tcaagcgccg aggtaggttc atcaaacagt
    35281 aaaacttccg gttccatcgc cagcgcccgc gcgatagaaa cacgctgttg ctgaccgccg
    35341 gaaagatgca ccggatattt cccctgcgca cgttcgtcta tcccgacttt cgccagatac
    35401 ttcaccgccc gctcgcgcgc ttcctgcttg ctcaggccca acacctgaat cggcgcttcc
    35461 atgacgtttt ccagcaccgt catatggctc cagagattga agtgctggaa taccatcgtc
    35521 aggcgtgtgc gcagtaagcg cagttgattt ttatcggcga ctttgagttg accatctttg
    35581 tcgcgcacca gattgatcgt ctggccactg accacgatcg atccttcgct cggtttttcg
    35641 aggaagttaa tgcagcgcag aaaggtactt ttccccgatc ccgacgatcc gatgatgctt
    35701 attacatctc cggcattcgc ttgcagtgac acccctttca gcacttcgtg ttcgccatag
    35761 cgtttgtgca aatcgataac gtttaattta ttctcggaca tcgtattctc agtgcgttga
    35821 agaaggtttc acatgctgca accagcgttt ttccgctctg cgaaagaggc tgatcaggac
    35881 ataagagatg attaaataga gcaccgcggc aatgccgaag gcggtaaaag gttgatacgt
    35941 ggcggcgtta atatcgcggg ctattttcag cagatccggc accgtggccg taaacgccag
    36001 cgcagtagag tgcagcatca ggatcacttc attgctgtat gccggtaacg caatacgcag
    36061 cgcagaaggc aaaataatgc agcgatacat tttaaaggtc gagaagccat aggctctggc
    36121 ggcttcaatt tccccatgcg gaaccgaacg gattgcccca gcaaaaatct cggtagtgta
    36181 agcgcaggtg ttaagcgtca gcgccagcac ggtacagttc aggccactgc ggaagaaagc
    36241 gttaaggaat tcggttccct taacaatctc aagcgtgtac atgccggaat agaacaccag
    36301 caactgaaca tacagcggcg taccgcgaaa aatataggta aataaccaga ttggaaactg
    36361 gatgtattta ttactggaga cacgaccaat cgccagaaac agcgccagga ctccgcctat
    36421 cactaccgac aaaataagca gccacagagt gatcgccaca ccagtaaagc gataaccgtc
    36481 ggtccacagc agcggtttcc agtattcatg taagatttcg atcacaggtc agccctcttc
    36541 acacccacgg agtagcggcg ctcaaggaac agcagcacac cattggaaac agtggtgaaa
    36601 accaggtaaa tcacgccaca gacgatggcg aaatagaacg gttcccaggt acttttgcct
    36661 gccagttggg tggctttgac cacatcttcc aggccgagta acgaaaccag tgcggtagat
    36721 ttgaggatca cctgccagtt gttgccaatg cctggcaggg cgtaacgcat catcgccgga
    36781 aacatgatcc gccgaaacac ttgcccacga gtaaaaccga acgccgtcgc cgcctctata
    36841 tgtcctttcg gcactgccat aaaagcacca cggaacgttt cggtaaaata agcaccgtag
    36901 ataaaaccga gagtgataat accagcgacc atcggatcga tatcaatctg cccgacgccc
    36961 atcgcctccg tcaccgtgtt tagcgcaatc tgcaaaccgt agaaaatcag cagcatcaac
    37021 actaagtccg gcacgccacg aatcagcgtg gtgtaccctt cgaaaatcag ccccgaaagc
    37081 cgattttgcg agagcttacc gccagcgcca attaaaccga tgattacagc gagcactaca
    37141 gagctgatag ccagctccag cgtgacgagc gcaccctgta aaataacacc tgaaaaccca
    37201 tacaacatgc agcctgtcct gtcgtgtgtg gtgaattacc gcctcttgtc tccctccggt
    37261 tacccgaagg gagcgggctt tctttcaccg atggcgatta gccaccataa acatcaaaat
    37321 cgaagtactt tttcgctaat ttctcgtaag taccgtcagc gcgcatttcg gcaaaggctt
    37381 tgttcagtgc ttcgcgcagt tcgttatctt ctttacgcag gcccatgccg gtccctacgc
    37441 caaacagttt ttcatcttta acagacgggc caccgaattt gtaatcttta ccgacaggtt
    37501 gtttgaggaa accttcgctg gcagcgacct catcctggaa cgcggcatca atacgtccgg
    37561 cagtcaggtc agaataaatg ttgtcctgcc cctgatacga gacgatttca atgccttttg
    37621 gtgcccaatg ttcattaccg aacgtctcct gggtggtgcc ctgcaatacg cctacccgtt
    37681 tgcctttcag cgactcgact gtcggctgaa tgtcagaatt tttcgccacc accaaacgag
    37741 aatcggcagc gtacagtttg tcggtgaagg ctatttcttg ctgacgtttt tccgtaatgg
    37801 aaagcgatga catgatggcg tcaatcttct tcgcttttaa ggacgggatt aacgcatcca
    37861 gcggattttc gacaaacgta cattgcgtat tgatgcgttt gcataattcc tttgccagat
    37921 cgatatcgaa gccaaccagt tcgccttgtg aattttttga ttcaaatggc gcataggtcg
    37981 ggtcggtacc gatgcggatg ttttgcggaa tcgcagcaaa cgccgcagtt gcgctggaga
    38041 aggccagaac cagagagagc gatagcacca gttttttcat aatgtcctca actgacagtc
    38101 tttttatagg gaatatttac aggtttgtta cacttatcgt gccataaaat tgaccatcaa
    38161 ggcaaaatat cccgccccgt tagacgtatt atttttcatt aaaaatgctt aagtatgcat
    38221 ttcaatgcac cacgatagtg caacaaacct ggcgtgcacc atgatgatca ttccatcagg
    38281 tacagcttcc cagcgacgta tcagtcaccg tagacattaa agtcgaaata ctttttcgcc
    38341 atcttgtcgt aggtgccgtc ctgacgcagc tcgccaagcg ccttattgaa ggcagccgtc
    38401 agttcagcat catctttacg tagccctaca ccggtgccat caccgaagta ttttttgtct
    38461 tttactgatg agccagcaaa ggcgaaatct ttaccagcag gttgcttgag gaatccttcg
    38521 ctggcagcaa cttcatcttg taacgcagca tccagacgtc ctgcagccag atcggaatag
    38581 accaaatcct ggttggcata ggccaccaca tccacgcctt tgctacgcca ggtctcgtta
    38641 gcgtaagctt cctgggttga tccctgcagc acaccaacat gtttaccttt cagtgaatcc
    38701 agcgttggct gaatcggtga acctttggca gcaatcaaac gagaatctgc ggcgtacagc
    38761 ttgtcggaga aggcaatctc ctgctgacgt ttatcggtaa tggaaagcga cgaaataatg
    38821 gcgtcgattt ttttcgcttt cagtgagggg atcagcgcgt caaagtcact ggcaacccag
    38881 gtacatttca cctgcatccg tttgcacatc tcgttaccga gatcgatatc aaagccaaca
    38941 aaatcacctt tagcatcttt cgacgagaac ggcgcatagg tggtatcggt tccgatacgt
    39001 accgtctccg gtagcgccgc atagctggaa gccgctgcgg agagaccgac taacaaagac
    39061 agagcgagaa tcgacttctt catacataac cctcaagtga aaatggcttt tttatgttgt
    39121 gtattgtgtt gtgtgtttgc aggctctttc atgcaggtct tatgccatct tgacgacaac
    39181 agtaacattc aacgttaaat atgttaataa gacgttgcat tattgtcctg aagttgaaga
    39241 tagcaggtat agcggttgaa tcgcagcgtt tcgattgaat ggcagaaaca aaatgtcgag
    39301 gatttgatcg cggttgcaaa attgccctga aacagggcaa cagcggagtt atgcgccctg
    39361 ccagcgggca aagagatctt caggaagggt tatcgcaaac tggtcaagaa cacgattaac
    39421 cgtctgattt atcacatcat caagggattg cgggcgatga taaaacgccg gaacgggagg
    39481 cataatcacc gcaccgattt ctgccgcctg agtcattaaa cgcagatggc ctaagtgcaa
    39541 tggtgtttca cgcacgcaga gcaccaacgg gcgacgctct ttcagcacca catctgccgc
    39601 acgggtcagt aagccatcag tatagctatg gacaatgccg gaaagggttt tgattgaaca
    39661 gggtaaaatc accatcccta gcgtctggaa agaaccggaa gagatgctgg cggcaatatc
    39721 gcgcgcatcg tgcgtgacat cggctaatgc ctgcacttcg cgcagagaaa aatccgtttc
    39781 gagggataag gtctggcgcg ctgcctggct catcaccaga tgcgtttcga tatctgtgac
    39841 atcgcgcaga acctgtaata agcgcacgcc ataaatcgcg ccgctggcac cgctgatgcc
    39901 tacaatgagt cgtttcataa atagttgccc tgtcagactt gcaggcagac tttgcaggat
    39961 ttcggcggga gttgcaagtc agggtgccag accggcaccc tcagcgaagg catcatcctt
    40021 cgttatgcat ttcgagattt tccacttcgt tctgacgttg cactgctttg gcgtcatcat
    40081 tacgtaacgt atcgaggaaa tcgaggtagc cctgatcaac atctttggtg acgtagacgc
    40141 cgttgaacac cgagcattca aactgctgga tatccggatt ttcagcgcga acggcgtcga
    40201 tcagatcgtt cagatcctgg aaaatcaacc cgtcagcacc gatgatctgg cgaatttcat
    40261 caacttcgcg accgtgagcg atcagttccg tggcgctcgg catatcaata ccataaacgt
    40321 tcgggaagcg aatttccggt gccgcagaag cgaggtacac tttcttcgct ccggcttcgc
    40381 gtgccatctc gataatctgc tcagaagtgg tgccacggac gatggagtcg tcgaccagca
    40441 ggacgttttt atcgcggaac tcggcgcggt tggcattcag tttacggcgc acggacttac
    40501 gacgcagctg ctggcccggc atgataaagg tgcggccaac atagcggttt ttaacgaagc
    40561 cctggcggta cggtttgccc agaatacgag caatttccag cgcgatatca cacgaggttt
    40621 ctgggatcgg gatcaccacg tcgatatcca gatcttccca ttcgcgggca attttctcgc
    40681 ccagtttcgt gcccatattc acacgcgcgc tgtaaacgga aattttgtcg atgaacgagt
    40741 ccgggcgagc aaagtataca tactcaaaca ggcacggatt gctgaccgga ttgtcagcac
    40801 attgacgggt aaacaactgc ccttcttcag tgatgtaaat cgcttcgccc ggcgcgacgt
    40861 cacgcaggaa atcaaagccc agcgtatcga gcgctacgct ttcggaagcg accatatatt
    40921 ctgtacggtt ctcgtcaata tcacgttttc ccagtaccag cggacgaatc ccgtttggat
    40981 cgcggaaagc aaccataccg tggccgataa tcatcgccac acaggcatat gcgccgcgga
    41041 ttaagcggtt tgtggcagca atggcagcga aaatattgtc ggcttccagc gggtagtggc
    41101 ggaagttgtc cagctcgctg gcgaagatat taagcagaat ttccgagtcg gaagtggtgt
    41161 tgatgtggcg gcgtttttct tcaaacagtt ttttacgcaa ctcgtgagcg ttggtcagat
    41221 tgccgttgtg ggcaagcgta atgccatacg gagagttaac gtaaaacggc tgcgcttcag
    41281 aggcgctgga gctgccagcc gtagggtaac gcacatgacc aatgcccata ttgccctgca
    41341 aacgctgcat atggcgagct tcaaatacat cgctcaccag cccgttcgct ttacgcaaac
    41401 ggaagcagtt attggcatct atggtgatga tgccggcggc atcctgaccg cgatgctgaa
    41461 gcaccgttaa ggcatcataa atcgactggt taaccggcat aacaccggcg ataccgacaa
    41521 taccgcacat acgtcttttt cctcgttaag ccacatctca gagcacttac gctctgggca
    41581 agaaacttga cgagctttgc agataatcaa aaaaccatct gatgataaaa ctgaactgcg
    41641 ggatcagctg tgatttgctc cagtcttcgc ttttcgacac cccggtaaag gagtcgagaa
    41701 agaagagaat ggcggcaaca atcaacacac cgcgcaacgc accgaaacag acgcccagca
    41761 cccgatcggt gcctgacaac cccgttttct ccaccaactg gcctatcacg aagttcacga
    41821 tagcaccaac gatcagggta gcgataaaca gtaccgcgat ggcaatccca tttcgaacca
    41881 gttcgtcttc aaagcccgta aaccagactg acaggtaagt gtagtaatga ctggcaacaa
    41941 agaaagcaca accccatgtc accagcgata acgcttcacg aacaaagccg cggatcaggc
    42001 taaccagaga ggaaaaagca atcaccgcga ttatggcgta atcaatccag accatatgtg
    42061 tcccacgatt ttacgccctg tcatcctgtt cggggcgcat tctaacagaa aaagaaaacg
    42121 tttgcgtagg gatttccttc ccacgcatca ataaaaatgg cgctgaaaaa atattcaacg
    42181 ccatcgactt tttatgcctt tgcggcatcg tgcaatgcgt gtcggatgcg gcgtaaacgc
    42241 cttatccgac ctacggttct aacactgccc aggcctgata agacgcgcca gcgtcgcatc
    42301 aggcaagact gtattaattc ggcgtatagc ccattaccac gccacttaag ccagaaagtt
    42361 gcttcaactc acccagcgaa cctttcagct tatctttcga ggcatccggc ccaaccagaa
    42421 tacgggtaat tttaccctgc actggcgtgg atggcgacgt ataaacccga taaccggcac
    42481 cgcgcagctt accgacaatc tcattcactt tatcggcatt tttcagcgca cccagttgca
    42541 caacataggc tttacccgtt ggcgctgctt tttcttccac gaccggcttc ggttctggct
    42601 tcggcgcagg tggtgcttca accttcggtt taggcggctc caccggtttc ggcttcggtg
    42661 gggcgaccgg tgcaggttcc ggttcaaact cggtgttatt ggctgcaata gtggtcggat
    42721 cgagcgacgg tgctgcggca tcacctgccc gcacctcttc cgctgcgcct tccggcggct
    42781 gcgtcggcaa cgcctgggtg gcggctggca tcatatcagg ctcatcgcga tcgcccgctt
    42841 tcggcaccag cgggatagcc gcgaactcat cctgataatg ttttttctgc ccgtccagca
    42901 gccctggaag tacaatcacc cccagcgcca ccagcacgat cgtgcccact aaccgattct
    42961 gaaacttact tgccaccgct tctcctcgcg tcaatcactt ccatgacatg tgcgacagtg
    43021 tggaaagaac cacacaccag cacggtgtct tccgctttag cgtccgccat tgcggcatcc
    43081 catgcctgcg caacgctatc aaatgatttg ccgttaccca aatgctcaag cagttgttct
    43141 gccgtggcac cgcgcggccc ttccagtggc gcacaatacc agtcatcaac cacgcttttc
    43201 aaccaggcca gagttccggc aatatcttta tcatgtagca taccgataac cgccagcacg
    43261 cgcccgtttt tcggtagcgc tttcatacgc ccggtgagat attccgccgc atgtggatta
    43321 tgcgcgacat caaaaataac gcgtggcgac tcgctcacaa tctggaaacg tcccggcaaa
    43381 attgcgctgg caatcccgtc gcgaatggca ttttcactga cttccagccc gctggcacgc
    43441 agtgccgcca gcgctgttgc ggcattcggt tgcgggacaa gcggcaacgg cagattttcc
    43501 agcgtgccgt gagcatcgct aaacgcccag tcatgatcgg tgacggaata gttccactca
    43561 acgccccgac gttgtaacag tgcacctttt tcctgcgcca catcagcaat ggtagaaggc
    43621 atttccggct caccgacaat tgccggtttt gcgctgcgaa agatgcctgc tttctcgcga
    43681 ccaatacttt cgcgatctgg tcccagccag tcggtatgat ccagcgcaat gctgattact
    43741 acagccacat cggcatcgac aatattggtt gcgtccagac gaccgcccag ccctacttcc
    43801 agaatcacta cgtcaagttg tgcctgtttg aacagccaca acgccgacag cgtaccgtac
    43861 tcgaaatagg tcagggaaat atcaccgcgt gccgattcaa tctccgcaaa agaggcggtg
    43921 tgggccgatt ccggcaattc ctgcccctgc acacgtacgc gctcggtata acgcaccaga
    43981 tgcggtgaac tgtagacgcc cactttgtac cctgccgcca tcagaatcga ctccagcgta
    44041 cggcaggtgg tgcctttgcc attcgtaccc gcaacggtaa acacaaatgg cgctggtttc
    44101 aggacgccaa gacgcgccgc gacctggctc acgcgctcaa ggccgagatc gatagtttta
    44161 ctgtgcaggt tttccagata agaaagccac gaagccagag gcgacgcggc ttgaggagtg
    44221 cgtttgataa tcatggtatc cgctgattcg ttacggtgag aatagcaaaa gggcagagcc
    44281 agtggccctg cccttatcag ttatcaggcc tcaggttcct gatccggtac cgggggtacc
    44341 actacgcctt cacgcggcgc ttcaggattc ggcgctggca gattcatcaa cttcgccaga
    44401 atgctcgcca gtttcaggcg catttccgga cgacggacga tcatgtcgat cgcgcctttc
    44461 tcgatcagga attcactgcg ctggaatcct ggcggcagtt tttcgcgaac ggtctgttcg
    44521 ataacacgcg gaccggcaaa gccgattaac gctttcggtt cagcgatgtt gagatcgccc
    44581 agcatggcga aacttgcaga aacaccgccc atcgtcgggt cggtcagcac ggagatgtac
    44641 ggcaagccgc gctcctgcat ttttgccagt gccgcagagg ttttcgccat ctgcatcagc
    44701 gacatcagtg cttcctgcat acgtgcgcca ccagaggcgg agaagcagat cagcgggcag
    44761 ttatcttcca gcgcctgctc aacggcacgc acgaaacgtg cacccacaac agaccccatt
    44821 gaaccgccca taaaggcgaa ctcgaatgcc gcagcgacaa ccggcattcc atacagagtg
    44881 cctttcatca ccaccagcgc atctttttcg ccagtttctt tctgcgcaga tgccagacgg
    44941 tctttatact tcttggagtc acgaaacttc agcacatctt tcggctcaag ctcgctaccc
    45001 agctccacaa ggcttccttc atctaacagg ctatgcaggc gattacgcgc tgtcatacgc
    45061 atgtgatggt cacacttcgg acagacctca agattacgtt ccagctcagc gcggtataaa
    45121 acctgaccgc agctatcaca cttagtccac accccttcag gaatgctcgc cttgcgggtg
    45181 ggagtaatgt tgcttttaat tcgttcaatc cagctcatta gggacctttc tgtctgaacc
    45241 tggttcgatg ccagttttat ctttggggac gcataatgcc atttttgccc ccaacagacc
    45301 atgaatgttg cacattaaaa cataacagcc cgaaactttg gataaaaaag tggtcgaacc
    45361 gcggagttac tttttatttt gcggcgcgtg ccgcagcgcg tttgtgacgg attatttcga
    45421 tgacgcccgg caaaatagaa accacaataa tcccgacgat cagcagctta aggttatcct
    45481 gaaccatcgg gattgtaccg aagaaatagc ctgcgtaggt aaaaagcagt acccacaaca
    45541 gtgcgccgat cacgttataa gcggcgaaat gacggtacga catgtggccc attcccgcaa
    45601 caaacggggc gaacgttctg acgatcggca caaaacgggc gagaataatc gttttgccgc
    45661 catgtttctc ataaaactga tgggttttgt cgagataact gcgacggaaa attttcgaat
    45721 tgggattact gaacaacttt tcaccgaaca accgcccgat agtgtaattg accgcgtcac
    45781 ccacaatcgc ggcgatcagc atcagcacca ccatcatatg gacgttgaga tcgttggttt
    45841 ccagtgatgc caacgctccg gcaacaaaca gcaatgaatc acccggtaaa aacggcgtta
    45901 ccaccagacc ggtttcacag aacaaaatta agaacaaaat ggcataaacc cagacgccgt
    45961 actccgcgac cagctctgcc agatgcacgt caatgtgcag gataaaatcg atgaggaaat
    46021 aaatcaggtc catatttgcc tatgccttgt actcgtcatc tttcaggctg taactacgtt
    46081 ggctacagcc tgaaatgttc cgagcattat tctcgttagt ccgccagaaa tagcgggccc
    46141 attggcggtt ttggaagatc ataccggtca gggtaatcca ccgcgaccag atacagccct
    46201 tccgcttttg ccgttgctgc cgccagcgtt ctgtcctttg ccgccagcaa ctctgctatc
    46261 cagctctccg gctggttgtg ggcaccgact tccatcaggc tgccgacaat attcctgacc
    46321 atatgatgta caaaggcatt cgctttgata tctaccacca cataaggacc gtgacgcgtg
    46381 acattaatat gcataacgtt gcgccacggg gttcgggact ggcactgcac cgcacggaac
    46441 gaggtgaaat cattctcgcc cagcaagcat tgcgcagccc gatgcatccg ttcagcgtcc
    46501 agcggttcgt aaaaatgggt taccccttta ctcagtaccg ccgggcgcag ccgatgattg
    46561 tagatgatgt agcgataacg gcgagccgtg gcgctaaatc gggcatgaaa atcatcaggt
    46621 acagctttaa cccaacgcac ggcgatgtca ccaggtaaat tcgcatttac gcccaaggtc
    46681 cacgccgcgt ctttgcgctg cgcggtcgtt tcgaaatgca ctacctgccc ggtaccgtgt
    46741 acaccagcgt cagtacgccc ggcgcagaag acggtaatgg gttcgttcgc cacctgggag
    46801 agcgcctttt ccagcttctc ctgcacactg cggacttcat tctgccgttg ccagccgtaa
    46861 tacttactgc cgtcgtactc aatgcccagc gcaattttat aaactggcgg ttgttgctgg
    46921 tcggacatta gtacagatac tcctgcacca gtttctcggc gatttttact gccatcagcg
    46981 cgccgccaaa gcgaacgtta tcggccaccg accagaactg gacttgctcc ggcataccgt
    47041 agtcattacg cacgcagcca acagaaagat gcggcgtacc cgaagcatcc cctacctgag
    47101 tcgggaattc gttctcttca gagagcacaa tatcttcgcc ctgagcaaac gcatcacacg
    47161 cttcttctgc cgccagcgga cgcagtgctt caaagttgac catctgggca tgaccgtaga
    47221 ataccggtgc ctggacgacg cttgccgaaa tcatcagccc ttcgtcctgc aggattttgc
    47281 gtacttcgtc aacgatacga cgttcttcac gcacgctacc ttcgctatcc ggcaacaacg
    47341 gcagcatatt gaacgccagt tgacgaccga agaaatcttc ttcgtcaatc ggaatgccgt
    47401 tgagcaattt cgcactctgc cccgctaacg catcgaccgc ttttttgccc tgggcggagg
    47461 ctgaaatcag gctggtaaca ctgatacgcg acaaaccgcc ctgatcgatt aacggtttca
    47521 gtgccgccag cagctggctg gtcagactgt ctggtacggc gatgacattc cggttgcggt
    47581 aatcggtcag tacaaacggg tttacttccg gcaccaccag cggtacgtcg ggttcgagag
    47641 caaacagtcc gctgctgtcg atcaccaggc aacctgagtt ggtcgcttct tcaacccagg
    47701 cagcggtagc ttctttgcct gcgacaaaaa atgccagctg cgcctgcgtc cagtcgaatt
    47761 cagcggcatc ctgcacggtg attgtcttac caccaaagcg cagttgttcg cctgcgcttt
    47821 cgttacgtgc cagtgcataa atttccccaa ccgggaactg acgttcagcc agcgtttcaa
    47881 gcagggcttc gcccacagcg ccagttgcgc ccaggacggc aatgttccag ccttcagaca
    47941 tggtggttta ctccagaaat agcaaagctc cctgccaatg ttacagcaga gagcatgaag
    48001 aagagattaa cgtgccggat gatgaacggc gttaaaaccc agtttacaca gcaatgatgc
    48061 cgcactggcg tcatcacaaa ttacatacag agatgaccat tcacggcgct caagatagtt
    48121 tttgcgcagt ttatcgaact cacccggtat cccggcgact ttacgcagcg gtgcgtcatc
    48181 gcggcgcaca tcatacacca aatgcaccag ccttttcagc gtcggttgat cgagcgggcc
    48241 atgcagcgta atgcgaccaa actctggcgc gggcagtaat gtatccagcg caacgtgctg
    48301 ttcatgccca ataaacttgc tataggcttc aaacacttgc gtagtaccgc gtgctttacc
    48361 ttccagggta taacctgcga tatgcggcgt gccgatatcc acttttgtca gcaactccac
    48421 gttaagttcc ggttcgcctt cccagacatc cagcactacg cttaacttct ggccttcatt
    48481 caggcaggtc agcaacgcag tattatcgac gactgcgcca cggcaggcgt taatcagaat
    48541 cgctcccggc ttcaggctac ggatcagttt ttcatccgcc agatgcagcg ttttgtacgg
    48601 cccatctttg aagagtggcg tatggaaagt cagaatatcc gcatgctgaa ctaattcatc
    48661 cagcgagcgg aaatcaccct catccccacg gtcagcgcga ggcggatcgc aaagcaatgt
    48721 tttaatccct aacgcttcca gtcgcgcctg caatcgacgt ccaacgttac caacgcccac
    48781 gatccccacc gtacggtcgt gcagtgaaaa tccatcgcgt tcggcaagca tcagtagtga
    48841 ggagaaaaca tattccacca ccgcaatcgc attacagcca ggtgcagcgg aaaaaccaat
    48901 tcccgcctgc tttaaccatg cttcatcgac atggtcggtc cccgcggtgg cagtgccaac
    48961 aaatttaatg ggttttcctg ccagcaaaga ttcattcact ttcgtgaccg aacgcaccat
    49021 cagcgcatcc gcgtctgcca gttgagcgac ggggattgga cgcccgggaa ccgcggtcac
    49081 ctcacccaaa cggctaaata agtcgcgggc ataaggcata ttttcatcaa caaggatttt
    49141 cacgtttgtg ttacctgtat gagacgagag ttaaccggac aagtgtgcca taatctcgcg
    49201 gccaggcata cttgcgaaga tttcaggtat aaggatacgt aatgatacaa cctatttccg
    49261 gccctcctcc tgggcaacca ccaggtcagg gagataacct gccgtctggc gcgggcaatc
    49321 agcctttatc cagtcagcaa cgtacttcgc tggaaagctt aatgacgaaa gtgacctcac
    49381 tgacgcaaca gcaaagagca gaactgtggg cgggtatcag gcacgatatt ggtctgtcgg
    49441 gagattcacc gctgctttcg cgtcacttcc ctgccgctga acacaacctg gcgcaacgtc
    49501 tgctggccgc gcaaaaaagc cattctgccc gccagctttt agcgcaatta ggggagtatt
    49561 tacgtctggg gaataatcgt caggcggtca cggattatat ccgtcataac tttggtcaga
    49621 cgccgctgaa tcagctctca ccggagcaat taaaaaccat tctcaccctg ttgcaggaag
    49681 ggaagatggt tattccgcaa ccgcagcagc gcgaggcgac cgaccgtcct ttattaccgg
    49741 cggagcacaa tgcgctcaaa cagctggtga ccaaacttgc ggcggcaacg ggggaaccca
    49801 gcaagcagat ctggcaatcg atgctggaac tatccggggt gaaagatggc gagttaattc
    49861 cagcgaaact gtttaaccat ctggtgacct ggttgcaggc gcgccagacg ctaagccagc
    49921 aaaatacgcc gacactggaa tcactacaga tggcgctaaa acaaccttta gatgccagtg
    49981 aactggcggc gttatcggca tatatccagc aaaaatatgg cctttctgcg caatcatcgc
    50041 tttcttctgc ccaggccgag gatattctta atcagcttta tcaacggcgg gttaaaggga
    50101 ttgatccgcg tgatatgcaa ccgctgctta atccttttcc accgatgatg gacacgttgc
    50161 aaaatatggc aacgcgtccc gcgctgtgga tactgttagt cgcgattatc ctgatgctgg
    50221 tctggctggt tcgttagccc cgacgaaatg acagtatcgt gacaataatt cccagcaccg
    50281 cagagatcgc cccggcaaga aataccgaag agtaaccaaa cgtggtcgcc agcattcccg
    50341 ccagcggccc ggagacgccg agggcgatat cctgaaacgc ggcgtaaccg cccagtgcgg
    50401 tgccgcgaac ttgtgagggg acgcgtttaa ccacctccac gcccagcgca ggaaagataa
    50461 gcgaacatcc ggctccggtt aacgccgcgc ccgctaatgc gacccacgca cctggggctt
    50521 gccagagcag caacaagccc accgtttcta caagcagaga gacaatcgcc actttcacgc
    50581 cgccaaaacg gtccggcatc cagccaaaca tgacgcgcat cacgacaaat gcgccgccaa
    50641 acgcggtaag agtaaagccc gccatcgccc atcctttgct ggcaaagtag agcgaaacga
    50701 aagtcccgat aaccgcaaaa ccaacgcctt gtagtgccag ccctaaccct ggtttccaga
    50761 taaacccgac aacgctccac agcgatggac gttctcccgc cagggccggt actttgcgca
    50821 ctgtgccgtt acaggcccac gccagtaagg gtaataccat tgtggtgatc gccagtgcgg
    50881 caaaaccgta atggctatga atcaacaggc caagcggagc accaacagcg agggcaccgt
    50941 aaatcgccat tccattccat gacatcactt tgccagagtg ttttggccct acgatgccta
    51001 acccccaggt cagagcgcct gtcagtaact ggctttcacc aaacccaaga atcaaacgcc
    51061 cgatgaccaa cagggcaaat ttgaacggtg cggagacagg caaaatcgcc gccagcagca
    51121 acgcgccgcc agccagacca caagctaaca tcccctgaag cgccgaacgt tttgcaccat
    51181 attgatcggc cagtcgcccg gcatagccac gcgtcagcac cgtagccaga aactgaatcc
    51241 cgacggcaat cccgaccatg gtattgccat agcccagatc atgatgaaca aacagcggga
    51301 taaccggcaa cggcagccct acggtcatgt aggtgagaaa aaccgcaaaa gcgatgcgga
    51361 agagcgaaaa attggcagaa gatcgtgttt cggtttggct tacagcagtc atgcattact
    51421 ccagaatgca gcgcaaggcg aggagtatcc ccgtctcatc tctctggttt cagggttaca
    51481 gtgcgttggc aggatttaac gcgtacgtct tttcagaagg aaatcgacaa agcgggaagt
    51541 ttgcctggaa ctggcggcga ttgtcaatga tgtgaaaaag ggaaccatca ggttcccttt
    51601 tgcgttagtg ccggaggccg cattggcgcg taacgtcgga tgcgacgctg gcgcgtctta
    51661 tccgacctac tccgactact ccgactactc cgacctactg cgaattaatc tttcagcttg
    51721 cgcattacca gcgtggcgtt ggtgccgccg aagccgaagc tgttagacat aacggtggtc
    51781 agttcgcgat cggtcgtttc ggtcacgatg ttcagacctg cagcctgctc gtccagctct
    51841 tcaatgttga tgctcggggc gataaagccg tgttccagca tcagcagaga gtagatagct
    51901 tcctgtacgc cagcagcgcc cagagagtga ccggtcatgg ctttggttgc agaaatcgcc
    51961 gggctcttat cgccgaacac ttcacggata gctgccagtt ctttcacgtc gccaaccgga
    52021 gtcgaagtac cgtgagagtt caggtaatcg attggggtat caacgccgtg catcgccatc
    52081 ttcatgcagc gtactgcgcc ttcgccagac ggagcaacca tgtctgcacc atcagaggtt
    52141 gcgccgtagc caacgatttc agcatagatg tgagcaccac gcgccagcgc gtgttccagc
    52201 tcttcaacca ctaccatacc gccgccgcca gcgataacga aaccgtcacg gtgagcgtcg
    52261 taagtacggg aggctttttc cggggtgtcg ttgtatttag tagacagcgc acccatcgcg
    52321 tcgaattcgc aggccatttc ccagcacagc tcttcgccgc cgccagcaaa cacgatgtcc
    52381 tgtttgccca gttggatctg ctctactgcg ttaccgatac agtgtgcgga agtcgcacac
    52441 gcggagctga tggagtagtt aacgccatga attttaaacg gcgtggcgag gcaggcagaa
    52501 acgccggatg ccatcgcttt ggtgaccaca tacgggccaa ccgctttcag gccgcgcggg
    52561 ccgcgcattg cgtcagcgcc gaacacctgg aaacgcgggg aaccgccgcc agaacctgca
    52621 atcaggccaa cgcgcgggtt attctggtaa gcttccggag aaaggcccgc atctgcgatt
    52681 gcctgctcca tagaaaggaa tgcataaatg gatgcgtcgc tcataaagcg cacaactttg
    52741 cggtcaatga ggccagtggt atccagtttt acgttgcccc agacgtggct acgcatgccg
    52801 gaatccttca gctcctgaga gaaagtgatc cctgaacgtc cttcacgcag agatgccagg
    52861 acttcctgct ggttattacc gatgctggaa acaatgccca ggccagtaat cactgcacgt
    52921 ttcattcaat acctctgtaa gtcgcacata gagtaagttt cgaatgcaca atagcgtaca
    52981 cttgtacgcc gaacaagtcc gatcagccat ttaatagaga aatttgcgca gccttacaca
    53041 catcgctaag atcgagccac cgcctgtaag acgagtaact tacgtgaaac actactccat
    53101 acaacctgcc aacctcgaat ttaatgctga gggtacacct gtttcccgag attttgacga
    53161 tgtctatttt tccaacgata acgggctgga agagacgcgt tatgtttttc tgggaggcaa
    53221 ccaattagag gtacgctttc ctgagcatcc acatcctctg tttgtggtag cagagagcgg
    53281 cttcggcacc ggattaaact tcctgacgct atggcaggca tttgatcagt ttcgcgaagc
    53341 gcatccgcaa gcgcaattac aacgcttaca tttcattagt tttgagaaat ttcccctcac
    53401 ccgtgcggat ttagccttag cgcatcaaca ctggccggaa ctggctccgt gggcagaaca
    53461 acttcaggcg cagtggccaa tgcccttgcc cggttgccat cgtttattgc tcgatgaagg
    53521 ccgcgtgacg ctggatttat ggtttggcga tattaacgaa ctgaccagcc aactggacga
    53581 ttcgctaaat caaaaagtag atgcctggtt tctggacggc tttgcgccag cgaaaaaccc
    53641 ggatatgtgg acgcaaaatc tgtttaacgc catggcaagg ttggcgcgtc cgggcggcac
    53701 gctggcgaca tttacgtctg ccggttttgt ccgccgcggt ttgcaggacg ccggattcac
    53761 gatgcaaaaa cgtaagggct ttgggcgcaa acgggaaatg ctttgcgggg tgatggaaca
    53821 gacattaccg ctcccctgct ccgcgccgtg gtttaaccgc acgggcagca gcaaacggga
    53881 agcggcgatt atcggcggtg gtattgccag cgcgttgttg tcgctggcgc tattacggcg
    53941 cggctggcag gtaacgcttt attgcgcgga tgaggccccc gcactgggtg cttccggcaa
    54001 tcgccagggg gcgctgtatc cgttattaag caaacacgat gaggcgctaa accgcttttt
    54061 ctctaatgcg tttacttttg ctcgtcggtt ttacgaccaa ttacccgtta aatttgatca
    54121 tgactggtgc ggcgtcacgc agttaggctg gaatgagaaa agccagcata aaatcgcaca
    54181 gatgttgtca atggatttac ccgcagaact ggctgtagcc gttgaggcaa atgcggttga
    54241 acaaattacg ggcgttgcga caaattgcag cggcattact tatccgcaag gtggttggct
    54301 gtgcccagca gaactgaccc gtaatgtgct ggaactggcg caacagcagg gtttgcagat
    54361 ttattatcaa tatcagttac agaatttatc ccgtaaggat gactgttggt tgttgaattt
    54421 tgcaggagat cagcaagcaa cacacagcgt agtggtactg gcgaacgggc atcaaatcag
    54481 ccgattcagc catacgtcga ctctcccggt gtattcggtt gccgggcagg tcagccatat
    54541 tccgacaacg ccggaattgg cagagctgaa gcaggtgctg tgctatgacg gttatctcac
    54601 gccacaaaat ccggcgaatc aacatcattg tattggtgcc agttatcatc gcggcagcga
    54661 agatacggcg tacagtgagg acgatcagca gcagaatcgc cagcggttga ttgattgttt
    54721 cccgcaggca cagtgggcaa aagaggttga tgtcagtgat aaagaggcgc gctgcggtgt
    54781 gcgttgtgcc acccgcgatc atctgccaat ggtaggcaat gttcccgatt atgaggcaac
    54841 actcgtggaa tatgcgtcgt tggcggagca gaaagatgag gcggtaagcg cgccggtttt
    54901 tgacgatctc tttatgtttg cggctttagg ttctcgcggt ttgtgttctg ccccgctgtg
    54961 tgccgagatt ctggcggcgc agatgagcga cgaaccgatt ccgatggatg ccagtacgct
    55021 ggcggcgtta aacccgaatc ggttatgggt gcggaaattg ttgaagggta aagcggttaa
    55081 ggcggggtaa tctgctctcg cagtgtttgc cggatgcggc gtgaacgcct tatccgacct
    55141 acgtgtgacg tgtaggcctg ataagacgcg ataagcgtcg catcaggcat ggtgctccag
    55201 acgccgcaac ttactgctgt gacgcctgct gaaacaggtt ttcccacata tcggtcacca
    55261 gcgcctggtc acgcggcgac aattcaccgg caccaatggc tttttccaga ctctggctaa
    55321 cggtcgtatg caccgcctga gcggagtggt catcaccact ttccagttct gcgatggcta
    55381 atgtcaggtg gccacgcaaa tacccactgg caaacaactc atcatcactg gcatggtcaa
    55441 ccataccgtc gattaatgcc agaatgcgtg attcaaactc cgcgatcatc ttctttcctc
    55501 attgtaaaac gtggcgctgg ctttagttga gcgcttccgg ccacgggaac tgttccgccg
    55561 taagttccgg cgtgtgataa taattttgta gtgctttaat gaagcgtgcc gggcgttcag
    55621 gaatgccgtt tgctaggtaa tccatcacct gcgcatgtac ccgccgttga aacaccacac
    55681 gatccggttc gaagtcgcct tccagattgt cgcaactgac gttaaacgga tatcccgccg
    55741 ccacacagaa caaccagtcg agtgcctgcg acttcacttc aacatcttca aactggcttt
    55801 gcgtttgggc atcacgccca tccgggcaat accagtagcc gaaatcaacc agctcacggc
    55861 gcgcttttcc ggcaatacac cagtgcgaaa tctcatgaat tgcgctggca taaaagccgt
    55921 gggcaaagac aattcggtta tacggtactt ccgcatcagc aggaagatag atcggttcgt
    55981 cgtcgccttt aatcagacgg gtattaaatt catcggcaaa gcagctatta aaaatttcaa
    56041 tcaactgctc atagtggtgt gtactgttca ttagtttatc cccaaccagt ggaggatctc
    56101 ctgtccgtgg ctatcgtaaa gtaatttggc actcatcacc gccgagacga taacaatcat
    56161 cgggcggatc agcttttgtc ctttgctcaa caccagtcgt gaacccatgc gcgcgccgag
    56221 gaactgcccc accagcatga caaagcccgt cgcccaaatc actttgccac cgagaataaa
    56281 cagcagtaaa ccgccgatgt ttgacgttgc gttgagtaat ttggcgtgcg ccgtggcttt
    56341 ggcgaggttg aatccgcaca gcgtaacaaa ggccagtgcg taaaacgatc cggctgccgg
    56401 accaaagaat ccgtcataaa aaccgacgca gccaccggca attaacgcga agggtaaacc
    56461 gtacatccga cgctggcgat cttcttcacc cagctttggc atcagcaaga aatagaggcc
    56521 gatacaaatt accagaatgg gcaaaatctg ccgtaagaca tcagcctgaa cgtattgcac
    56581 cagcagtgcg ccgctcattg agccgacaaa ggtcatggcg atattgagtt tctgatcgct
    56641 taaactaacc actttgcggc gaataaagta gatagtggcg gaaatagagc cgccgcaggc
    56701 ttgcagttta ttggttgcca gcgcattagc gggagacatc cccgcagcca tcaatgccgg
    56761 aatggtgagt aacccaccgc caccggcaat cgagtcgata aatcccgcca gcatggcgac
    56821 aaaaaagaga actcccagca acagcgggga aaccataaac aggctattaa acgtttccat
    56881 tagatcacgt gctcatccag tagcgcctgg caggaaggcg gcaacggagg cggtgtcttc
    56941 ttctcaggct ttgttgttcc cggttttgga ggttcaaacc agctttgcag ttctgccccg
    57001 caaccatcgc ctggtggcgg taaaggttga tcttcacact ccagactatc ggcaggacaa
    57061 cgtaatcgta catgcatatg cgcgcgatgc tggaaccagg gtcgcacttt gcgcaaccag
    57121 tcgcgatcgg tgcccgcatc aaggcaaagt tgttgtttaa tcgccggatt aacaaaaatg
    57181 cgcgtgacgt ctttgtcctg ggcggcgagt ttgatcaagc tgaaaatttc tggcttccac
    57241 agcgtggaga caacgtgttt accgtcgcgg gaaactaagt ccagtgcttg cgggcgcaag
    57301 agctgcgcgg aggtccagcg agttttcggc agttgcagaa agatatcgac atccagtccg
    57361 gtctggtggc tggcatgacc gccgttgaaa cgccctccag cgggcatccc catatcgcca
    57421 atcagcaccg tacccatgcc cagattgctc acctggctac tcagacgctg gataaacatc
    57481 accaggtccg ggtgaccgaa atagcgacgc tgatcggtac gcatgacctg ataatgttcg
    57541 gactgtatcg gcagcgtgtc agcgccgacg atacagccat tagaaaaact gcctatcgat
    57601 tgtgcgctac ccggcacagg ttgggttatt ttttgccacg gcgtcgctgc caggctggcg
    57661 ctactggcaa gcagagccag cagcgcaatc gcggttttat tcatttttta ccagcgtgga
    57721 atatcagtct tcacatcggc attttgcgcc cgctgccgta gcaggtgatc cattaaaacg
    57781 atcgccagca tcgcttcagc gatcggcacc gcgcggatcc cgacacaggg atcgtgacga
    57841 cctttggtga tcatctcaac ttcttcgcca aagcggttaa tagtccgccc cggcacggta
    57901 atgctggagg tcggtttcag cgccatatgg gcaatgattt gctgcccgct gctgataccg
    57961 ccgagaatgc cgcccgcatg gttgctctgg aaaccgtctt tggtgatttc gtcgcggttc
    58021 tggctgccgc gcagcgccac tacatcaaaa ccgtcgccaa tttccacgcc tttcaccgcg
    58081 ttgatgctca tcagcgcatg ggcgatgtcg gcatccaggc ggtcaaagac cggctcgcca
    58141 agtccggcgg ggacgccact agcaacaacg gtgactttcg cgccgatgga gtcgccctct
    58201 tttttcagcg cgcgcatcag ttcatctaac gcgtcgattt tgtcggggtc cgggcagaag
    58261 aacggatttt gttcaaccag cgaccagtct ttgatttcca gcggaatgtc gcccatctgg
    58321 gtcaggcagc cgcggatttc aataccaaat ttctcggcga gatatttttt ggcaatcgcc
    58381 cctgccgcca cgcgcatggc ggtttcgcgg gcggaagaac gtccaccgcc gcgataatcg
    58441 cgcagaccgt atttttgttc gtaggtgtaa tcggcatggc ctggacggaa aacgtcttta
    58501 attgcgctgt aatcctgaga acgctggtcg gtattttcga tcaacaagcc aatgctggtg
    58561 ccagtagtaa cgccttcaaa aacaccggag agaattttga cctgatccgg ctcgcggcgc
    58621 tgggtggtat agcgcgatgt cccagggcga cgacggtcga ggtcatgttg caggtccgct
    58681 tccgtgagcg gaatgcctgg cggaacacca tcgacgatgc agccgagcgc cagcccgtgc
    58741 gattcgccga aggtggttac gcgaaagagt tgtccaattg tgtttccagc catcacggct
    58801 ccgttattgt tgtgtttgcg tgtttactta atctttataa atcgcgaaat gttcacgggc
    58861 cgccagcagt tgctctttgg tgagcataaa cacaccatcg ccgccgttat caaactccag
    58921 ccaggtgaac ggaacatccg gatattgttc cataagatgt accatgctgt tgccgacttc
    58981 acaaatcaac acgccatcat ccgcaaggta atctgccgcg tttccgagaa tgcgacgcgt
    59041 cagtttcagg ccgtcggtgc cagatgccag gcctagttcc ggctcgtgac ggtattcgtt
    59101 tggcaggtcg gacatatctt ccgcatcgac atacggcggg ttagtgacaa tcaggtcgta
    59161 ctgcactttc ggcaagtcgc ggaacagatc ggaacgaatc ggaatgacgt tgtggatcag
    59221 accgtgttct tcgatgttct gttcggcaac cgccagcgcg tcgggagaga tatctaccgc
    59281 gtcgacttcc gcttccggga aggcataagc acaggcaatg gcgatgcagc cgctaccggt
    59341 acacatatct aaaatatgct gcggttgctt gctgatgagt ccggcaaatt tattgttgat
    59401 cagttcacca atcggcgagc gcggcaccag cacgcgttca tcgacgtaaa attcatgacc
    59461 gcagaaccag gctttgttgg tcaggtaagc caccggaatg cgctcgttga cgcggcggat
    59521 cacgcgttca acaatacggt gtttttcgct ggaggtcaga cgcgcggtgc gcatatcttc
    59581 cgggatatcc agcggcaggt agagcgatgg taacaccagc tgtacggctt catcccacgg
    59641 gttatcggtg ccgtgaccgt accagatatt tgccgcgctg aagcggctta ccgaccagcg
    59701 caacatgtcc tgaatggttt gcagctcatt tactgcttca tcaacgaaaa ttttatccac
    59761 gtattcctcc agggcatgat cgcaataatt tcggcggcta gtttgccatg aagatgacga
    59821 taaatcagca ttcacgcgcg gtgagtgagg aaaaatacgt ttaaaacgat cgattgtgct
    59881 acgagtcggg taaactgtag gaaaattaga aatagagaca gataaatgaa aaagaaaaca
    59941 acacttagcg aggaggacca ggctctgttt cgccagttga tggcggggac tcgcaagatt
    60001 aagcaggaca cgattgtcca ccgaccgcag cgtaaaaaaa tcagcgaagt gccggtgaaa
    60061 cgcttgatcc aggagcaggc tgatgccagc cattatttct ccgatgagtt tcagccgtta
    60121 ttaaataccg aaggtccggt gaaatatgtt cgcccggatg tcagccattt tgaggcgaag
    60181 aaactgcgcc gcggcgatta ttcaccggaa ttgtttttgg atttacacgg tctgacgcag
    60241 ctgcaggcca agcaggaact gggcgcgttg attgccgcct gtcgccgcga acatgtgttt
    60301 tgtgcctgcg tgatgcatgg gcacgggaag catattttga agcaacaaac accgctgtgg
    60361 ctggcacaac atccgcatgt gatggcattt caccaggcac cgaaagagta cggcggtgat
    60421 gcggctttgt tggtgttgat tgaagtggaa gagtggttgc cgccggagtt gccctgaatg
    60481 ctggcagcaa atgccggagc agcgtaaacg ccttatccgg cctacaaata tgtgataatt
    60541 caataaattg caattcact
//
LOCUS       DAZGSP010000015        60295 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZGSP010000015 DAZGSP010000000
VERSION     DAZGSP010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.015"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(314..1705)
                     /gene="narK"
                     /locus_tag="ACHK74_001080"
     CDS             complement(314..1705)
                     /gene="narK"
                     /locus_tag="ACHK74_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFP0219940.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTHVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            2044..3840
                     /gene="narX"
                     /locus_tag="ACHK74_001081"
     CDS             2044..3840
                     /gene="narX"
                     /locus_tag="ACHK74_001081"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFP0219941.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            3833..4483
                     /gene="narL"
                     /locus_tag="ACHK74_001082"
     CDS             3833..4483
                     /gene="narL"
                     /locus_tag="ACHK74_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFP0219942.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(4484..5878)
                     /gene="ychO"
                     /locus_tag="ACHK74_001083"
                     /gene_synonym="iatA"
     CDS             complement(4484..5878)
                     /gene="ychO"
                     /locus_tag="ACHK74_001083"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFP0219943.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGERASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            6063..6416
                     /gene="ychN"
                     /locus_tag="ACHK74_001084"
     CDS             6063..6416
                     /gene="ychN"
                     /locus_tag="ACHK74_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFP0219944.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(6460..7155)
                     /gene="chaC"
                     /locus_tag="ACHK74_001085"
     CDS             complement(6460..7155)
                     /gene="chaC"
                     /locus_tag="ACHK74_001085"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFP0219945.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     EPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVSAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENFPDGVLRPGFA"
     gene            complement(7313..7543)
                     /gene="chaB"
                     /locus_tag="ACHK74_001086"
     CDS             complement(7313..7543)
                     /gene="chaB"
                     /locus_tag="ACHK74_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFP0219946.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            7813..8913
                     /gene="chaA"
                     /locus_tag="ACHK74_001087"
     CDS             7813..8913
                     /gene="chaA"
                     /locus_tag="ACHK74_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFP0219947.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            9318..9425
                     /locus_tag="ACHK74_001088"
     CDS             9318..9425
                     /locus_tag="ACHK74_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0219948.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            9854..9961
                     /locus_tag="ACHK74_001089"
     CDS             9854..9961
                     /locus_tag="ACHK74_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0219949.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            10389..10496
                     /locus_tag="ACHK74_001090"
     CDS             10389..10496
                     /locus_tag="ACHK74_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0219950.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(10645..11499)
                     /gene="kdsA"
                     /locus_tag="ACHK74_001091"
     CDS             complement(10645..11499)
                     /gene="kdsA"
                     /locus_tag="ACHK74_001091"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFP0219951.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(11535..12344)
                     /gene="sirB1"
                     /locus_tag="ACHK74_001092"
     CDS             complement(11535..12344)
                     /gene="sirB1"
                     /locus_tag="ACHK74_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFP0219952.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(12348..12740)
                     /gene="sirB2"
                     /locus_tag="ACHK74_001093"
     CDS             complement(12348..12740)
                     /gene="sirB2"
                     /locus_tag="ACHK74_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFP0219953.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(12737..13570)
                     /gene="prmC"
                     /locus_tag="ACHK74_001094"
     CDS             complement(12737..13570)
                     /gene="prmC"
                     /locus_tag="ACHK74_001094"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP0219954.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(13570..14652)
                     /gene="prfA"
                     /locus_tag="ACHK74_001095"
     CDS             complement(13570..14652)
                     /gene="prfA"
                     /locus_tag="ACHK74_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFP0219955.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(14694..15950)
                     /gene="hemA"
                     /locus_tag="ACHK74_001096"
     CDS             complement(14694..15950)
                     /gene="hemA"
                     /locus_tag="ACHK74_001096"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFP0219956.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            16164..16787
                     /gene="lolB"
                     /locus_tag="ACHK74_001097"
     CDS             16164..16787
                     /gene="lolB"
                     /locus_tag="ACHK74_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFP0219957.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            16787..17638
                     /gene="ispE"
                     /locus_tag="ACHK74_001098"
     CDS             16787..17638
                     /gene="ispE"
                     /locus_tag="ACHK74_001098"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFP0219958.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            17789..18736
                     /gene="prs"
                     /locus_tag="ACHK74_001099"
     CDS             17789..18736
                     /gene="prs"
                     /locus_tag="ACHK74_001099"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP0219959.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCIL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            18861..20540
                     /gene="dauA"
                     /locus_tag="ACHK74_001100"
     CDS             18861..20540
                     /gene="dauA"
                     /locus_tag="ACHK74_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFP0219960.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(20595..20873)
                     /gene="ychH"
                     /locus_tag="ACHK74_001101"
     CDS             complement(20595..20873)
                     /gene="ychH"
                     /locus_tag="ACHK74_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFP0219961.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            21151..21735
                     /gene="pth"
                     /locus_tag="ACHK74_001102"
     CDS             21151..21735
                     /gene="pth"
                     /locus_tag="ACHK74_001102"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFP0219962.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            21852..22943
                     /gene="ychF"
                     /locus_tag="ACHK74_001103"
     CDS             21852..22943
                     /gene="ychF"
                     /locus_tag="ACHK74_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFP0219963.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            23784..26669
                     /gene="ycgV"
                     /locus_tag="ACHK74_001104"
     CDS             23784..26669
                     /gene="ycgV"
                     /locus_tag="ACHK74_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0219964.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVL
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTDYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQSNLFDQKQVNGGYRFSF"
     gene            complement(26769..28688)
                     /gene="dhaR"
                     /locus_tag="ACHK74_001105"
     CDS             complement(26769..28688)
                     /gene="dhaR"
                     /locus_tag="ACHK74_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFP0219965.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMSPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDVQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            28916..29986
                     /gene="dhaK"
                     /locus_tag="ACHK74_001106"
     CDS             28916..29986
                     /gene="dhaK"
                     /locus_tag="ACHK74_001106"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFP0219966.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLCFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            29997..30629
                     /gene="dhaL"
                     /locus_tag="ACHK74_001107"
     CDS             29997..30629
                     /gene="dhaL"
                     /locus_tag="ACHK74_001107"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFP0219967.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGVDGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            30640..32058
                     /gene="dhaM"
                     /locus_tag="ACHK74_001108"
     CDS             30640..32058
                     /gene="dhaM"
                     /locus_tag="ACHK74_001108"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFP0219968.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            32378..34075
                     /gene="treA"
                     /locus_tag="ACHK74_001109"
     CDS             32378..34075
                     /gene="treA"
                     /locus_tag="ACHK74_001109"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFP0219969.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(34154..34594)
                     /gene="ycgY"
                     /locus_tag="ACHK74_001110"
     CDS             complement(34154..34594)
                     /gene="ycgY"
                     /locus_tag="ACHK74_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFP0219970.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(34772..35026)
                     /gene="ymgE"
                     /locus_tag="ACHK74_001111"
     CDS             complement(34772..35026)
                     /gene="ymgE"
                     /locus_tag="ACHK74_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP0219971.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            35227..35961
                     /gene="ycgR"
                     /locus_tag="ACHK74_001112"
     CDS             35227..35961
                     /gene="ycgR"
                     /locus_tag="ACHK74_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFP0219972.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(35963..36574)
                     /gene="emtA"
                     /locus_tag="ACHK74_001113"
     CDS             complement(35963..36574)
                     /gene="emtA"
                     /locus_tag="ACHK74_001113"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFP0219973.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            36674..37588
                     /gene="ldcA"
                     /locus_tag="ACHK74_001114"
     CDS             36674..37588
                     /gene="ldcA"
                     /locus_tag="ACHK74_001114"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFP0219974.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            37683..39419
                     /gene="cvrA"
                     /locus_tag="ACHK74_001115"
     CDS             37683..39419
                     /gene="cvrA"
                     /locus_tag="ACHK74_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFP0219975.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(39805..40875)
                     /gene="dadX"
                     /locus_tag="ACHK74_001116"
     CDS             complement(39805..40875)
                     /gene="dadX"
                     /locus_tag="ACHK74_001116"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFP0219976.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(40885..42183)
                     /gene="dadA"
                     /locus_tag="ACHK74_001117"
     CDS             complement(40885..42183)
                     /gene="dadA"
                     /locus_tag="ACHK74_001117"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFP0219977.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            42513..44045
                     /gene="ycgB"
                     /locus_tag="ACHK74_001118"
     CDS             42513..44045
                     /gene="ycgB"
                     /locus_tag="ACHK74_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFP0219978.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(44097..44816)
                     /gene="fadR"
                     /locus_tag="ACHK74_001119"
     CDS             complement(44097..44816)
                     /gene="fadR"
                     /locus_tag="ACHK74_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFP0219979.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            45038..46579
                     /gene="nhaB"
                     /locus_tag="ACHK74_001120"
     CDS             45038..46579
                     /gene="nhaB"
                     /locus_tag="ACHK74_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFP0219980.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            46725..47255
                     /gene="dsbB"
                     /locus_tag="ACHK74_001121"
     CDS             46725..47255
                     /gene="dsbB"
                     /locus_tag="ACHK74_001121"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFP0219981.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(47301..48569)
                     /gene="umuC"
                     /locus_tag="ACHK74_001122"
     CDS             complement(47301..48569)
                     /gene="umuC"
                     /locus_tag="ACHK74_001122"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFP0219982.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(48569..48988)
                     /gene="umuD"
                     /locus_tag="ACHK74_001123"
     CDS             complement(48569..48988)
                     /gene="umuD"
                     /locus_tag="ACHK74_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFP0219983.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            49361..50271
                     /gene="hlyE"
                     /locus_tag="ACHK74_001124"
                     /pseudo
     CDS             49361..50271
                     /gene="hlyE"
                     /locus_tag="ACHK74_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            complement(50478..50924)
                     /gene="ycgN"
                     /locus_tag="ACHK74_001125"
     CDS             complement(50478..50924)
                     /gene="ycgN"
                     /locus_tag="ACHK74_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFP0219984.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRDNLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(51016..51675)
                     /gene="ycgM"
                     /locus_tag="ACHK74_001126"
     CDS             complement(51016..51675)
                     /gene="ycgM"
                     /locus_tag="ACHK74_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFP0219985.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIIPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(51747..52040)
                     /locus_tag="ACHK74_001127"
     CDS             complement(51747..52040)
                     /locus_tag="ACHK74_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFP0219986.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            52282..52683
                     /gene="pliG"
                     /locus_tag="ACHK74_001128"
     CDS             52282..52683
                     /gene="pliG"
                     /locus_tag="ACHK74_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFP0219987.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(52803..53171)
                     /gene="ycgJ"
                     /locus_tag="ACHK74_001129"
     CDS             complement(52803..53171)
                     /gene="ycgJ"
                     /locus_tag="ACHK74_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFP0219988.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            53691..54386
                     /gene="minC"
                     /locus_tag="ACHK74_001130"
     CDS             53691..54386
                     /gene="minC"
                     /locus_tag="ACHK74_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFP0219989.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            54410..55222
                     /gene="minD"
                     /locus_tag="ACHK74_001131"
     CDS             54410..55222
                     /gene="minD"
                     /locus_tag="ACHK74_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFP0219990.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            55226..55492
                     /gene="minE"
                     /locus_tag="ACHK74_001132"
     CDS             55226..55492
                     /gene="minE"
                     /locus_tag="ACHK74_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFP0219991.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(55864..56208)
                     /locus_tag="ACHK74_001133"
                     /pseudo
     CDS             complement(55864..56208)
                     /locus_tag="ACHK74_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(56243..56362)
                     /locus_tag="ACHK74_001134"
     CDS             complement(56243..56362)
                     /locus_tag="ACHK74_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP0219992.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(56323..56508)
                     /gene="ymgJ"
                     /locus_tag="ACHK74_001135"
     CDS             complement(56323..56508)
                     /gene="ymgJ"
                     /locus_tag="ACHK74_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFP0219993.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            56609..56782
                     /gene="ymgI"
                     /locus_tag="ACHK74_001136"
     CDS             56609..56782
                     /gene="ymgI"
                     /locus_tag="ACHK74_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFP0219994.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            56784..57128
                     /gene="ymgG"
                     /locus_tag="ACHK74_001137"
     CDS             56784..57128
                     /gene="ymgG"
                     /locus_tag="ACHK74_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFP0219995.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            57132..57467
                     /gene="ymgD"
                     /locus_tag="ACHK74_001138"
     CDS             57132..57467
                     /gene="ymgD"
                     /locus_tag="ACHK74_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFP0219996.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(57524..59845)
                     /locus_tag="ACHK74_001139"
     CDS             complement(57524..59845)
                     /locus_tag="ACHK74_001139"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0219997.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     GSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(59962..60171)
                     /locus_tag="ACHK74_001140"
     CDS             complement(59962..60171)
                     /locus_tag="ACHK74_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0219998.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
BASE COUNT        15307 a        15244 c        14655 g        15089 t
ORIGIN      
        1 acctgtagtt atttaaagca tgaagatttt tatgcagatg ggtattaagg agtattcccc
       61 atgggtaaca tattaatggc tcttaatgaa atccggcgaa taagccatat aacattagta
      121 tgttgtaact aaataacgca taaaaaaagc gcgacatcat gccgcgcaaa ggataatcaa
      181 atattttact ctatataatt cggtttacag gaaggcggca agtgaatgaa tcccaggagc
      241 ttactaaagt aagtgactgg ggtgaatgaa cgcagccgca gcacatgtaa cccgaagtat
      301 gacgagtaac ggtttatttt ttagaatgcc gaccatatac cgcccaggta atcactacgc
      361 aggcgatata gaaaatcaaa aataccttca ttgcgccgac tggcgaaccc gttaatgcca
      421 gcgagctacc aaacgctttc gggataaaga agccaccaat agcgccaatc gcagagatga
      481 aacccagcgc cgctgccgtg tcggttgccg cttcacgcat cgcacgttcg tcagaacccc
      541 cttctgcttt cacgcgatcc attgtcagtt tacggaagat cactgaaatc atctggaaag
      601 tggaaccact acccagccca gctgtcagga acagcgccag gaagaccgcg aagaacgcca
      661 tgaagcttcc gccctgcccg tcagtcggta aggtcaggaa cagcaggccg ctgaaaatcg
      721 ccatcagaat aaagttcacc agcgtgacat gagttccgcc cagacggtca gataatgcac
      781 cacctgcaga acgcgccagc gcaccaataa acggcccgaa gaaagcgtat tgcagaatct
      841 gaacatccgg gaactgcgtt tttgacagca ttgcaaagcc cgcggagaag ccgatgaagg
      901 agccgaaggt tgccagatac agcaggctca taatccacag atgaccccgt ttgagtaccg
      961 gcaactgctc cttgatggag gctttcgagg tagcaagatc gttcatgcca aaccacgccg
     1021 caatagtgaa gatggcaagg aacggcaccc atatccagga cgcattcgcc agatacagct
     1081 cagtcccatc cggctgtttg acgccctggc taccaaatac tgcgaaaatc gacagtgata
     1141 ccaccagcgg agcaaccaac tgcatgacgc tgacgcccat gttgcccaga ccaccattca
     1201 gacccagcgc gccaccctgc ttctgtttcg gaaagaagaa gctgatgttt gccatactgg
     1261 aggcgaagtt cgcgccagca aagccgcata gcagagagat gatgatgaag acgctataag
     1321 gcgtggaggt atcctgcacg gcaaaaccca gccagacgca aggaataatc agaataccgg
     1381 tgctgaacgc cgtccagcga cgaccaccga agataggaac cataaaggag tatggaacac
     1441 gtaataacgc gccagaaacc gaaggcagcg cagtcaacat aaatagctga tcggtcgtaa
     1501 aattaaagcc gactttcggt aggttcaccg caacagcgct gaacaacatc catacgcaaa
     1561 acgccagcag cagacaggga acggaaatcc acaggttgcg gctggcaata cgttgaccgc
     1621 gttgttgcca gaacgcagga tcttccggtc gccaatctgt aatgacagct ccagtagccc
     1681 tttcgggggc ggatgagtga ctcatagaca cctctgatac tcgtttcgat gtctgccacc
     1741 ttagtgtctg tagctaaagg caatttgatg taaatcaaac gataagataa ctttatctat
     1801 cattgatatt tatcattacc catagtgagt acagtgattt cataaaaatt atgagatttt
     1861 tcacggtgct gtagaatccc tacccttacc gatgtaaagc gactaaccac acggcaaata
     1921 aggagtaact ctttccaggt atgggtatac ttcagccaat agccgagaat actgccattc
     1981 cagaatgtat cgtcacattc attaaggtta ttgctcattt aaagcctgaa ggaagaggtt
     2041 tacatgctta aacgttgtct ctctccgctc accctggtta atcaggttgc gcttattgtg
     2101 ttgctttcta ctgctattgg actggcaggg atggcggttt ctggctggct ggtgcaaggc
     2161 gttcagggca gcgcccatgc gatcaacaaa gcgggatcgc tgcgcatgca aagttaccgt
     2221 ctgttggcag cagtgccatt aagcgagaaa gacaagccct taattaaaga gatggaacaa
     2281 acggcattta gcgccgagtt gactcgagca gcagaacgag acggacaact ggcgcaatta
     2341 cagggtttac aagattactg gcgtaatgaa ctgatccctg cgttgatgcg tgcacaaaac
     2401 cgagaaacgg tgtcagcgga tgtcagccag tttgttgccg ggcttgatca actggtatct
     2461 ggttttgacc gcaccacgga aatgcgcatc gagacagtgg tactggtcca tcgggtaatg
     2521 gcggtattta tggcactttt actggtgttc actattatct ggttgcgggc gcgactgcta
     2581 caaccgtggc ggcaactgct ggcaatggcg agtgccgtca gtcatcgcga ttttacccaa
     2641 cgcgcaaaca tcagcgggcg caacgaaatg gcgatgcttg gaactgcgtt gaacaatatg
     2701 tctgcagaac tggccgaaag ttatgccgta cttgagcagc gggttcagga gaaaaccgcc
     2761 gggctggagc ataaaaatca gatcctctct tttttatggc aggctaaccg ccgtttgcat
     2821 tcccaagccc cgctgtgtga acgcctgtca cctgtactca acggcttaca gaatttaacc
     2881 ctgctacgtg atatcgaact gcgggtttat gatactgatg atgaagagaa tcatcaggag
     2941 tttacctgcc aaccagatat gacttgtgat gataaaggct gccagctctg cccgcgcggc
     3001 gtattacccg ttggtgatcg cggcacaacc ctgaagtggc ggctggctga ctctcatacg
     3061 cagtacggta ttttgctggc gaccctgccg caggggcgtc atcttagcca tgatcaacaa
     3121 caactggtgg ataccctggt tgaacaactc accgccacgc tggcgctgga tcgccatcag
     3181 gaacgtcagc aacagttgat cgtgatggaa gagcgtgcca ccattgcgcg cgaactgcat
     3241 gattctattg cccaatctct ctcttgcatg aagatgcagg tgagttgttt acagatgcag
     3301 ggcgatgcgc tgccagaaag cagccgcgaa ctgttaagtc agatccgtaa cgaactgaat
     3361 gcatcctggg cgcagttgcg tgaattgctc accacattcc gcttgcagct caccgagcct
     3421 ggattacgtc cggcgctgga ggcgagttgc gaagagtaca gcgccaaatt tggcttcccg
     3481 gtgaagctgg attatcaatt gccgcctcgc ctggtgcctt cgcatcaggc aatccacttg
     3541 ttgcaaattg cccgtgaggc attaagtaac gccctcaaac attcgcaagc gagtgaagtc
     3601 gtggtgacgg tggcgcaaaa cgataatcag gtcaaactga ccgtccagga taacggctgc
     3661 ggcgtgcctg aaaatgccat ccgcagcaat cactacggca tgataataat gcgcgaccgt
     3721 gcgcaaagtt tacgaggcga ttgccgcgtc cgccgtcgtg aatcaggtgg caccgaagtg
     3781 gtggtcacct ttattcccga aaaaactttc acagacgtcc aaggagatac ccatgagtaa
     3841 tcaggaaccg gctactatcc tgctgatcga cgatcacccg atgctgcgaa ctggcgtaaa
     3901 acagcttatc agtatggcac cagatatcac cgtggttggc gaagcgagta atggcgaaca
     3961 gggtattgaa ctggcggagt ctcttgatcc cgatctgatc ctgttagatc tcaatatgcc
     4021 cggcatgaac ggtctggaaa cgctggataa actgcgcgaa aagtccctct cagggcgcat
     4081 tgtggtattc agcgtctcta accatgaaga agatgtggtc accgcactga aacgcggcgc
     4141 ggatggttat ctgttaaaag atatggaacc ggaagatctg ctgaaagcat tgcatcaggc
     4201 agctgctggc gaaatggtat taagcgaagc attaacgcct gttctggccg ccagcttgcg
     4261 cgctaaccgt gccactactg agcgcgatgt taaccagtta accccacgcg agcgcgatat
     4321 tctcaagctg attgcccagg gtttgccgaa caagatgatt gcccgccgcc tggatatcac
     4381 cgaaagcaca gtaaaagtgc acgtcaagca catgctgaag aaaatgaagc tcaagtctcg
     4441 cgtggaagca gcggtatggg tgcatcagga gcgcattttc tgattacggt tcccagcgca
     4501 gttcgtcgtt tgacaatgca tcgaacggtt cgacaagcgt tagcgtgatc tcattggagg
     4561 agacacgctg cccctggtta tcttccacca ccacagacaa tcgccagtga ttgctcgccc
     4621 tttccccgtt ctgccagtca ggcatgatca gcgtccagcc ctccgcgctg ttggcttgtg
     4681 cgcccggcgt caaacttaat atctgcgtat cgccctgcca aatcagttgc cgaataccgt
     4741 aacgactgcg gatttgtaat ttcagcggca ctgtttcgcc aggttttaga tcccacggcg
     4801 gtgtcgccag aaacaccgtt aacgtttttc gctgtcggta ctcaagagtc ggtagattat
     4861 ttcgctgcgg attgtcatag cgactaccac gtaacgactg actttcggca acctcacccg
     4921 cagaaagttg ttttttgagc ggtacaccaa agcggtaatt aagattcagc ccgaggttat
     4981 tttgattctc gccactttca ccctgtttat gctgggccgt cacagtgact aatggcacag
     5041 gggtgtaatt taatcccaga ctcaacgcga cgggattgtg ataacccgta ccagagttaa
     5101 acaaatcaac acgatcacca aaatactgtt ctacgctgac actggtattg aggtgttgat
     5161 agaacggcat gcgcatccgg gctgtcaggt catacccgcg cgccatccgt tgttcctgcg
     5221 tggctgtctg ttcatgccat gcagcaaacg gctgataaaa gtttgccgat agtcgcaaat
     5281 attcgcccca cgcttcggca ccaaagcccg ctcgctgaag attttcgtcc agcaagttgt
     5341 cataaaaagt gttataaccc accagccagt tgccgcgcgc ccagcgttgc ccaacgccca
     5401 cattgctcac caacccatca tcctgctgag taagaccaag ctggctccag gtgagataac
     5461 gatcattatc ttgtaacggc acaaaccagc ttccacgact gccggtgaaa tgtccttcgt
     5521 tatccacttt gacgtcaaca ctggcatttc cccacggtga tagccaggac tctacgtgct
     5581 gattaacctg ttgactaagc gcgtcgcgga cttttcccaa tgcgaaagct tttgcctgct
     5641 cgccagtatc cagcccgtta tcattcatac tggtttcgcc gaaatctttc acaatttcag
     5701 caaagtgttt ttccccatca tgattttcgg gagccatgcc taaatccggc agaccatcat
     5761 tgttattatc aaagggattt gccgcttttt gctcgaaggt agattgtgcg ttagctgtac
     5821 cgcctgctac aagcaagagt aaataaaacg ggataatgcg aggaacgaaa cggctcaaaa
     5881 cgtcggtaac ttccggtaat aatgaaaaac catagcctta aagaataacg attactggca
     5941 acttttgtcg tattttctcg cgctttcagg aaaatcctta aaaatgtgac cgattggtca
     6001 gacgctgcag aagcagtaag atagaagatg agtgtctaat tccctgaata caggagtaaa
     6061 taatgcaaaa aatcgtgatc gttgccaatg gcgcacctta cgggagcgaa tccttgttta
     6121 acagcttgcg gctggccatt gcgttacgag agcaggagag caatctggat ctgcgtctgt
     6181 tcctcatgtc tgatgcggtc acagccgggt tgcgcgggca aaaaccaggg gaaggctaca
     6241 acattcagca aatgctggag atccttaccg ctcagaatgt accggtgaaa ttgtgcaaaa
     6301 cctgtaccga cgggcgcggg attagtacac ttcctctgat tgatggggtg gaaatcggta
     6361 ctcttgtgga actggcgcaa tggacgctgt cagccgataa agtgctcaca ttttaatgtt
     6421 tgagggggcg tctgacgccc cactatgcgg gaagtttatt caggcgaatc ccggacgtaa
     6481 cacaccatcc ggaaaattct ccgccagcag tttttttacc gataccagca aatcattcag
     6541 cccatcgtcc tgcattccca gtttgataag ctcctgttcc agtgaaaaca ggtattgtgc
     6601 gttggttccc agcggaccgc tcgccgccgc aatcaacggc gcgatgacct gagcgcgagt
     6661 atcagattca tattctggat gtcgcggatc cataataaac acaatggcgc ttactgtgcg
     6721 tccatcatca agatcaagct gacaccaggt tggcagataa cagccggtaa tcatctctcg
     6781 cttccacaac aaggttagtt cctgctccag cgtctcttct ggcagtcgat aggcgacgcc
     6841 tgtggtgcgt ccgccctctt tcagtgcaag catccgtccc ggctggtgcg cagtcccgcg
     6901 cccggcggtc aggcgcaggc aaaatgcgcg atgccatcca accagtgtac cggtgcacga
     6961 ttcggtaaac tccagtgccg gattccacat caacgatcca tagccgaaga tccacaccgg
     7021 ttcctcatca ggtcgacaag ccagcgtcgc cgccagcgaa gccgcccgtt gttctgctga
     7081 ccataagagt gattcttcaa tggcaccaaa tgccgtttta caatcggcat tcatcaagaa
     7141 atcacgcgtt atcaccttac acctccgcca ctgcatactt ccctgcgaca actttttggt
     7201 gtcttccgga catttttact gctcattttg aaaccactaa cgggacaata tgcaatccac
     7261 ggaccacttg caaggcaaca ccgcacgaat agctttaact aagctaccgg ttttacgatt
     7321 ttttatgcca tttatcatca tcccctttgg catattcatg cttcacagca gcccaggcca
     7381 ctttatgcgc ggtttcttcg cgactggcgt catcacgccg atcgtcttta tctttatatt
     7441 gatcccatgc gctgttgaac gcttctttat agatatcctg ggcatgagac ggtagaacgt
     7501 gctttacgct ttccggcaga tcgcttttcg ttttatacgg catcgttgac tccttttaca
     7561 aagttgaaca ttaagtgtgg tcaacaatat ctactcttgc caaaacataa gaattttcat
     7621 gtcagactct tcttaagaaa cgctaaaaca aacattgtct aaacgtaata cttattttta
     7681 tgccccaatg agtaaaaaat cgacagcggg gtaaaaattc gtaaaatgtt accgatatcc
     7741 agacactttc tgttattttc cctacctatt ttttacattt tcgataaatt catctgcaaa
     7801 gggataacca taatgtcaaa tgctcaagag gcggtaaaaa cccgccacaa ggagacttcg
     7861 cttattttcc cggttctggc gctggtagtg ctgttcctgt ggggaagcag ccagacacta
     7921 ccagtggtca ttgccatcaa tcttcttgcg cttattggta ttttaagtag cgcctttagt
     7981 gttgtccgtc atgcggacgt attagcccat cgcctgggag aaccttacgg ttcgcttatt
     8041 cttagccttt cagtggttat tcttgaagtc agtttgattt cagctttaat ggcaaccggc
     8101 gacgccgcgc caacgctaat gcgtgatacg ctctattcaa tcattatgat tgttaccggt
     8161 gggctggttg gcttttcatt attgttgggc ggtcgtaagt ttgccaccca atatatgaat
     8221 ctgtttggta tcaagcagta tttaattgcg ctgttccccc tggcgataat cgtactggta
     8281 tttccaatgg ctctgcctgc ggcgaatttt tcaaccggtc aggcgttact ggtagcatta
     8341 atttctgcgg caatgtatgg cgtatttttg ctgatccaga ccaaaacgca tcaaagtttg
     8401 tttgtctacg agcacgaaga tgacagtgat gacgacgacc cgcatcacgg taaaccgtct
     8461 gcccatagca gcgtgtggca tgctatctgg ttgattatcc atctgattgc cgttattgca
     8521 gtcaccaaaa tgaatgccag cccgctggag acattgctcg acagcatgaa tgcccctgtc
     8581 gcctttactg gcttcctggt ggctctgttg attctgtcgc cggaaggttt aggtgcgtta
     8641 aaagcagtgt tgaacaacca ggtccagcgc gcgatgaatc tgttctttgg ttcagtgtta
     8701 gcaaccattt cgcttaccgt acctgtcgtc acgctaattg cctttatgac gggtaacgaa
     8761 ttacagtttg cacttggtgc gccagaaatg gtggtgatgg tggcctcttt agtgctgtgc
     8821 catatctcct tctccaccgg acgtactaac gtgctcaatg gcgcagcgca tctggcactg
     8881 tttgccgcct atttgatgac gatatttgcc tgacagttat atctcctccg atgacggaaa
     8941 tcgggggaga taagataaat tcctgcttat tgtatttctt catactacct ggacagctcg
     9001 ttaatcagaa ttgcgagcct gtaccacaac ctgcacgaaa ccactgcaac agctgttgaa
     9061 aagccggaat gtactcttta ttgcaaacat ttcagggatg aagtgaagat gaattgagac
     9121 gtagtatcca caccaagccg gaattatccg gtgaggcgca atgttgcggg ggctttatcc
     9181 ctggtggcat tggttgctgg aaagagaaaa cccccgcacg ttgagaggta aaacctgaca
     9241 acaaaacggg ggctaatctt gaaaccagac acttcaagaa tagccgcaaa atgttgtcat
     9301 tacaacaagg cggctatatg acgctcgcgc agtttgccat gactttctgg cacgacctgg
     9361 cagcaccgat cctggcgggg attattaccg cagcgattgt cagctggtgg cgtaaccgga
     9421 agtaaggtga aaacgggcgt caggctgccg tgatggcaat atgcgcccga aaccggccgc
     9481 aggggtaaca gcctgcggcc atttttgtct ttatctcttc cacaagtaaa tattggcaac
     9541 ccgagctgga agcatcatcc agagtactca tgaaacgact gtaacagctg ttgaaaaacc
     9601 ggaatgcact cttcattgca aacatttcag ggatgaagtg aagatgaatt gagtcgtagt
     9661 atccacacca agccggaatt atccggtgag gcgcaatgtt gcgggggctt tatccctggt
     9721 ggcattggtt gctggaaaga gaaaaccccc gcacgttgag aggtaaaacc tgacaacaga
     9781 acgggggcta atcttgaacc atcaacaatt caagaatagc cgcaaaatgt tgtcattaca
     9841 acaaggcggc tatatgacgc tcgcgaagtt tgccatgatt ttctggcacg acctggcagc
     9901 accgatcctg gcggggatta ttaccgcagc gattgtcggc tggtggcgta accggaagta
     9961 aggtgaaaac gggcgtcagg ctgccgtgat ggcaatatgc gcccgaaacc ggccgcaggg
    10021 gtaacagcct gcggccattt ttgtctttat ctcttccaca agtaaatatt ggcaacccga
    10081 gctggaagca tcatccagag ccctcatgaa acgactgtaa cagctgttga aaaaccggaa
    10141 tgcactcttc attgcaaaca tttcagggat gaagtgaaga tgaattgaga cgtagtatcc
    10201 acatcaagcc ggcgttatcc ggtgaggcgc aatgttgcgg gggctttatc cctggtggca
    10261 ttggttgctg gaaagagaaa acccccgcac gttgagaggt ttaacctgac aacagaacgg
    10321 gggctaatct tgaaccagaa caattcaaga atagccgcaa aatgttgtca ttacaacaag
    10381 gcggctatat gacgctcgcg cagtttgcca tgattttctg gcacgacctg gcagcaccga
    10441 tcctggcggg aattattacc gcagcgattg tcggctggtg gcgtaaccgg aagtaaggtg
    10501 aaaacgggcg tcaggctgcc gtgatggcaa tatgcgcccg aaaccggccg caggggtaac
    10561 agcctgcggc cttttttgta tctggcaccc atgaaaaaag tcttaacgca gaacgctaag
    10621 actttatttt tcaagcaaaa aagattactt gctggtatcc agttcttcga aacctttcac
    10681 cagatcatca atcgctttca tctgcttgag gaacggttcc agtttagcca gcggcagcgc
    10741 ggatggacca tcacatttcg catgttccgg atccggatgc gcttcaataa acagccccgc
    10801 cagacctacc gccataccgg ctcgtgccag ctcagccacc tgagcacgac gaccaccgga
    10861 agctgcgcca aacggatcgc ggcattgcag tgcgtgggtc acgtcgaaaa tcaccggcga
    10921 gttaccagac actttcttca taatgctgaa gcccagcata tcgacaacca ggttgtcata
    10981 gccgaagtta gcaccacgat cgcaaagaat cactttttcg ttgccgcctt ctttgaattt
    11041 atcaacgata ttacccatct gtcccgggct gacaaactgt ggtttcttga cgttaattac
    11101 cgcaccggtt ttcgccatgg cttcaaccag gtcagtctgg cgagcaagaa acgccggcaa
    11161 ctgaatcaca tccacgacat cagcaacggg ctgtgcctga cttggttcgt gaacgtcggt
    11221 gataattttc acgccaaaag tctgcttcaa ctcctggaag attttcatcc cttcttccag
    11281 gcccggtcca cgataagagt ggatggagga gcggttggct ttgtcaaaag aggctttgaa
    11341 cacgtaaggg atacccagtt tctgggtcac agttacgtag tgctcgcaaa tgcgcatcgc
    11401 cagatcgcga gattccagca cgttcatacc gccaaacagt acgaacggca ggtcatttgc
    11461 tacgttgatg tcgccaatgc taaccacttt ttgtttcata ggatcgcctt aatcttgagt
    11521 aaaatgtcga ttaattaatg cagcacaata tgtttatgcg cgatgttatt tatttgcgca
    11581 cggatcattt cgctgatcgg gtcttccgga cactgttcaa cgaaataact taaatcgttc
    11641 aacgcaacgt gttcgcaatc cagttgcgca taaatcaacc cgcgatcgcg aatttcatag
    11701 ggatcttcag ggttgaattg taataaagct tcgctggtgc gtaacgccag ctccatctga
    11761 ttttcttcca tcaacgaggc tttgagtgta tccagcaatt tgcggattac ctcaatgtta
    11821 tcagcttcat caaggtcttc ataaaacagt tccgccgacg ggctgatatt tccctttaac
    11881 catacgtcca gcatatgttc gcttaacgat tcaccgttaa aaggattaat cagccaaatt
    11941 tcgccatccg gacattcaat gcgcaatatc agctgcgtag ggaaaatcac cggcagcagc
    12001 ggcaaatcga gacgattcgc gacccataat aaaaccgcac ctaatgatac cgcactgccc
    12061 tgtcgattct ttaacacctg gtccagccat aatgcatcgg aaagacgata aacgccgcgt
    12121 gaggctttaa atccccagtc gccgtaaaac agcgcgatca gtttttccag ctgctcttct
    12181 aaaggcagaa gctggctgat ttcttccttc gccagactaa cgagacgctc cagttcgtcg
    12241 taaacatctt gcgagggaaa atcgcggcgg attgcttcgc aagccaggat catgccttcg
    12301 cacaatggcg ctttattaaa ttcgaaatca gctaacgatc tcatgactta ccccagtaac
    12361 ggtacttttg tggtggcgag tttaatgatg atgtacagca ccaccaacgc cagcgggaag
    12421 gcaataatgc gcgcctgctg actgtgcata cgacgataat cgagtgcaat aaaacccaaa
    12481 acgatataaa tgataactcc aaacagcttt tcagtcagcc actgtgcctg cccggaaaat
    12541 ggcaggatgt gcgctttagc catcaacgca atgccgctta acaataacac cgtatcgaca
    12601 accggcggca caatgcgcgt ccagcgagca aatgcctgtg gatgcttctg ataacgtagc
    12661 cagaaacgta aggttaatag cccaacagaa agcgcgatac taataagatg aacactaagc
    12721 agtgtagaaa aacttgtcat tgataatagc ggccgagcgt tacgcgctcg ttatcaccat
    12781 agtcacggca ggtttcgacg tcatgatacc ccgcgaggat aaatgcttgt cgcaccgctt
    12841 cgccctgctg ccagccatgt tccagaagca gaaagccgcc ggataccagc gcgttacgcg
    12901 actgttcgat gatatgcacg atgtctgcca ttccactgtc tgccgcaacc agcgcagtga
    12961 gcggctcaaa gcggacatcg ccttgttgaa gatgtgggtc ctgctcgtca atatacggcg
    13021 gattgctgac aatcatcgca aactgctgcc cggctagcgc gctaaaccag tcgctttgca
    13081 gaatgtggat atttttgatc gccagatgct gggcattacg ttgtgccagg gagacagcat
    13141 caggcatacg atctacagcg ataatttcgc agtccgggcg ctcgctagcc agcgcaagcg
    13201 caatcgcccc ggtacccgtc ccgagatcga gaatacggca aggttgttca ggcaaccgcg
    13261 ccagtgcctg ctccaccaga cactccgtat ccgggcgcgg aattaaggtc gctggcgaaa
    13321 caaataacgg caacgaccag aattctcgca ccccggttaa atgagcaatg ggttcaccat
    13381 cgcgacgacg tgtcagtagc gcatcaagtt gctgacattg ttcgtcagtc agctgcgttt
    13441 caccaaaggc cagaataaaa gtccgccccc tgccagtgac atactccaat aagatttcag
    13501 cgtcacggcg tgggctttcg ctcgcctgaa gttggcttat tgcttcacgt aaccagtgtt
    13561 gatattccat tattcctgct cggacaacgc cgccagttgg tcggcctgat gttcctggat
    13621 aatcggttca atcagcatat ccagcttacc ttccatcact tcatccaggc ggtagagcgt
    13681 caggttgatg cggtgatcgg taacgcgccc ctgcgggaaa ttgtaagtac ggttacggtc
    13741 gctgcgatcg ccactcccca gcaggttacg acgggtagac gcttcggcct gttggcgttt
    13801 tgccatttca gcagcgtgga tgcgagcacc gagcactgaa agcgccttcg ctttgttttt
    13861 atgttgtgaa cgttcatcct gacattcaac aacaatcccg gtcggcaagt gagtaatacg
    13921 aattgccgaa tcggtggtgt taacgtgctg accacccgcc cctgacgagc ggaaagtatc
    13981 aatgcgtaaa tctgctgggt tgatgtccgg cagttctgcg tcaggcagtt ctggcattac
    14041 cgcaacggta caagcagaag tatgaatacg accctgcgat tccgtagcag gaacacgctg
    14101 cacgcgatga ccgccagatt caaatttcag acgaccatac acaccatcac cgctaatttt
    14161 ggcgatgatc tctttataac caccatgttc accctcgctg gcgctcatga tttctacccg
    14221 ccagcggcgg gcttcggcgt aacggctgta catacggaac agatcgcctg cgaacagcgc
    14281 cgcttcgtcg ccgccggttc cggctcggac ttcgaggaag gcgttacgtt cgtcatcagg
    14341 atcttttggc agtaacagaa cctgtaattg ctgttccagt tgctcgcttt tttctttagc
    14401 ttcgcgcagt tcatcctgcg ccatctcacg catttcagga tcatcgagca tcatctgtgc
    14461 ggtttcgata tcttcctgaa cctgttgcca gtcggtaaaa cagcgcgaaa catcacttaa
    14521 ctgcgcatat tcgcgtgata atgcgcgaaa acgttcctgg tcggcgatag tttgcgcgtc
    14581 acccagcaac gcctgaactt cttcatggcg ttcatgcagg gcttccagtt tggcaacgat
    14641 agaaggcttc ataggcgtaa atgcaccctg taaaaaaaga aaatgatgta ctgctactcc
    14701 agcccgaggc tgtcgcgcag aatattcagg cgttcgttat ccccgtcacg ggcggcctgt
    14761 tgaagtgatt tcgttggcgc atggatcaag cggttagtca gtttccatgc cagatcctgc
    14821 ataatggctt gcgcgtcgcc gccctgctca agggccgcta acgctttggc ggttaactca
    14881 tcgcgaacgt gctctgcctg gctgcgatac tcgcgaatgg tttcgctggc gctttgtgct
    14941 cgcagccacg ccataaattc gctggtttcc tgagcgacaa tagtttccgc ctcaaccgct
    15001 gcggctttac gctgcgccag gttgtgcgaa atgatgcttt gcagatcgtc cacactataa
    15061 agataagcat tcgccagttt gccaacttcc ggctcaacat cgcgcggaac ggcaatatcc
    15121 accaacagca ttggttggtt gcgacggctt tttaatgcgc gctccaccat gcctttcccg
    15181 ataatcggta acgggctggc ggtggaactg atgatgatat cggcttcgcg cagacgttcg
    15241 tcgatctcac tcagggcaat cacttccgcg ccaacttcat ctgccagaat ttgggcacgt
    15301 tcgcgagtgc ggttggcgat aatcatcttc tgtactttat gttcgcgcag atgacgcgct
    15361 accagctcga tggtttcgcc cgcgcctacc agcaacactg tgaccgtaga gagcgattca
    15421 aagatctgcc gcgccagcgt acaagccgca aaagcgacag acacagcact ggcaccgata
    15481 tctgtttcag tgcgaacgcg tttcgcgaca gagaaagatt tctggaacat gcgttccagt
    15541 tcgctggcct tcatatgacc tttttgcgaa tcggcaaacg cttttttaac ctgaccgagg
    15601 atctgcggct ccccaagaac cagtgaatcc aggccgctgg caacacgcat taaatggcta
    15661 accgcgtcgt tatcctgatg ccagtagagg cttttacgca gatcttcttc attaagattg
    15721 tgataatcgc aaagccagcg gattaacgcc tcttgcaggt tatcctgctc ttcaacgcta
    15781 agataaagtt ccgtgcggtt gcacgtcgac agcaccacgc cgccctgcac catcggctgc
    15841 gcaagcaggc tgtcaagcgc ctgatcgagc ttatccggcg aaaacgatac acgttctcgc
    15901 agcgatacag gtgccgtttt atggttgata ccgagtgcta aaagggtcat gtctgcggga
    15961 aataatacca acgttgatag ggttagtctg cttgcatcat acaggatgcg taagatcaat
    16021 aaaagagagc gccccctttt ggagtaattg ccgaaagccg ttagattctg gcaattaaga
    16081 caacttgaac atagacgata gcggacggta acgctagcat taagggttat aactgcaacg
    16141 tatctcaagg acttgtcatc actatgcccc tgcccgattt tcgtcttatc cgcctgctac
    16201 cgctggctgc tcttgtgctt actgcctgtt ccgttaccac gcccaaaggt cctggcaaaa
    16261 gcccggattc gccacaatgg cgtcagcatc agcaagacgt gcgcaatctt aatcagtatc
    16321 agactcgcgg cgcgttcgct tatatttctg accaacaaaa agtgtacgcc cgctttttct
    16381 ggcagcaaac cggccaggat cgctaccgtc tgctgctcac taacccattg ggcagcacgg
    16441 aactggagct gaatgctcaa ccgggtaacg tgcagttagt cgacaataaa ggtcagcgtt
    16501 ataccgccga tgacgccgaa gagatgattg gcaaattgac cggaatgcca attccgctca
    16561 acagcttgcg ccagtggatt ttaggtttac cgggtgatgc aaccgactac aaactggacg
    16621 accagtaccg cctgagcgaa attacctaca gccagaatgg caaaaactgg aaggttgttt
    16681 atggtggtta tgacaccaaa acgcaacctg cgatgccagc caatatggaa ctcaccgacg
    16741 gtggtcaacg catcaagtta aaaatggata actggatagt gaaataatgc ggacacagtg
    16801 gccctctccg gcaaaactta atctgttttt atacattacc ggtcagcgtg cggatggtta
    16861 ccacacgctg caaacgctgt ttcagtttct tgattacggc gacaccatca gcattgagct
    16921 tcgtgacgat ggggatattc gtctgttaac gcccgttgaa ggcgtggaac atgaagataa
    16981 cctgatcgtt cgcgcagcgc ggttgttgat gaaaactgcg gcagacagcg ggcgtcttcc
    17041 gacgggaagc ggtgcgaata tcagcattga caagcgtttg ccgatgggcg gcggtctcgg
    17101 cggtggttca tccaatgccg cgacggtcct ggtggcatta aatcatctct ggcaatgcgg
    17161 gctaagcatg gatgagctgg cggaaatggg gctgacgctg ggcgcagatg ttcctgtctt
    17221 tgttcggggg catgccgcgt ttgccgaagg cgttggtgaa atactaacgc cggtggatcc
    17281 gccagagaag tggtatctgg tggcgcaccc tggtgtaagt attccgactc cggtgatttt
    17341 taaagatcct gaactcccgc gcaatacgcc aaaaaggtca atagaaacgt tgctaaaatg
    17401 tgaattcagc aatgattgcg aggttatcgc aagaaaacgt tttcgcgagg ttgatgcggt
    17461 gctttcctgg ctgttagaat acgccccgtc gcgcctgact gggacagggg cctgtgtctt
    17521 tgctgaattt gatacagagt ctgaagcccg ccaggtgcta gagcaagccc cggaatggct
    17581 caatggcttt gtggcgaaag gcgttaatct ttccccattg cacagagcca tgctttaagc
    17641 cgggcaagct gagtttcggt gacaacgtca ccttgttcca gacgttgcat cgcgctcttt
    17701 aatacaccgc ctggaaagga tcatgcctgg cccgcacagt tttcggcaga ttctttccac
    17761 caatggacgc atgcctgagg ttcttctcgt gcctgatatg aagctttttg ctggtaacgc
    17821 caccccggaa ctagcacaac gtattgccaa ccgcctgtac acttcactcg gcgacgccgc
    17881 tgtaggtcgc tttagcgatg gcgaagtcag cgtacaaatt aatgaaaatg tacgcggtgg
    17941 tgatattttc atcatccagt ccacttgtgc ccctactaac gacaacctga tggaattagt
    18001 cgttatggtt gatgccctgc gtcgtgcttc cgcaggtcgt atcaccgctg ttatccccta
    18061 ctttggctat gcgcgccagg accgtcgcgt ccgttccgct cgtgtaccaa tcactgcgaa
    18121 agtggttgca gacttcctct ccagcgtcgg tgttgaccgt gtgctgacag tggatctgca
    18181 cgctgaacag attcagggtt tcttcgacgt tccggttgat aacgtatttg gtagcccgat
    18241 cctgctggaa gacatgctgc agctgaatct ggataaccca attgtggttt ctccggacat
    18301 cggcggcgtt gtgcgtgccc gcgctatcgc taagctgctg aacgataccg atatggcaat
    18361 catcgacaaa cgtcgtccgc gtgcgaacgt ttcccaggtg atgcatatca tcggtgacgt
    18421 tgcaggtcgt gactgcatac tggtcgatga tatgatcgac actggcggta cgctgtgtaa
    18481 agctgctgaa gctctgaaag aacgtggtgc taaacgtgta tttgcgtacg cgactcaccc
    18541 gatcttctct ggcaacgcgg cgaacaacct gcgtaactct gtaattgatg aagtcgttgt
    18601 ctgcgatacc attccgctga gcgatgaaat caaatcactg ccgaacgtgc gtactctgac
    18661 cctgtcaggt atgctggccg aagcgattcg tcgtatcagc aacgaagaat cgatctctgc
    18721 catgttcgaa cactaatcga acccggctca aagacccgct gcggcgggtt tttttgtctg
    18781 taatatccat ttgtatgacc tatgcctcct tcacctgcca tttagttgac agatgatgcg
    18841 ctcacggatg aaacattatt gtgaacaaaa tattttcctc acatgtgatg cctttccgcg
    18901 ctctgatcga cgcttgctgg aaagaaaaat atactgccgc acggtttacc cgtgacctga
    18961 ttgccgggat aaccgtcggg attattgcta tcccgctggc gatggcgttg gctattggta
    19021 gtggtgtggc accccagtac ggtttatata ccgcagctgt tgcggggatt gtcattgctc
    19081 tgacgggtgg gtcacgcttt agcgtttccg gtccgactgc ggcatttgtg gtaattctct
    19141 atcccgtgtc gcaacagttt ggactggcag gactgctggt tgcgaccttg ctgtcgggga
    19201 tctttttgat tctgatgggt ctggcacgct ttggtcgcct gattgagtat attccggttt
    19261 ccgtcacctt aggtttcacc tcgggtatcg ggatcaccat cggtaccatg cagattaaag
    19321 attttctcgg tctgcaaatg gcccatgtcc cggaacatta tctacaaaaa gtcggcgcat
    19381 tatttatggc gctgccgacc attaatgtgg gtgatgctgc cattggcatt gtgacgctag
    19441 gtattcttgt tttttggccg cgtctgggca ttcgtttacc cggtcacctt ccggccttgc
    19501 tggctggttg cgcggtgatg gggattgtta acctgctcgg cggacatgtt gctaccatcg
    19561 gttcgcaatt ccactacgtc ctggccgatg gttctcaggg taacggtatt ccgcaactgc
    19621 tgccgcaact ggtgctgccg tgggatctgc ctaattcaga attcacgcta acctgggatt
    19681 ctattcgcac actgctgcct gcggcattct caatggcaat gctcggcgca atcgaatctc
    19741 tgctctgcgc cgtggtgctg gatggtatga ccgggacgaa acacaaggcg aatagcgaac
    19801 tggttggaca gggactgggg aatatcatcg ctccgttctt tggtggcatt accgctacag
    19861 ctgccatcgc gcgttctgcc gctaacgtcc gtgccggggc aacttcccct atctcggcgg
    19921 tgatccactc tattctggtt attcttgccc tgctggtact ggcaccgctg ctctcctggc
    19981 tgccgctttc cgccatggct gccctgctgt tgatggtggc gtggaacatg agtgaagcgc
    20041 acaaagtggt cgacttgctg cgtcatgcgc cgaaagatga catcattgtc atgctgctgt
    20101 gcatgtcgct gaccgtgctg tttgatatgg ttattgccat cagcgtgggg atcgtgctgg
    20161 catcgctgct gtttatgcgt cgtatcgcac gtatgactcg cctggcaccg gtagtcgtag
    20221 atgttccaga cgatgtcctg gttctgcgcg ttattggccc gctgtttttt gctgctgctg
    20281 aaggcttgtt cacggacctg gagtcacgtc ttgaaggcaa acggattgtg attctgaagt
    20341 gggatgcggt tccggtactt gatgctggtg gtcttgatgc gttccagcgt tttgtgaagc
    20401 gtctgcccga gggatgtgaa ctgcgcgtgt gcaacgtgga attccagcca ctgcgcacta
    20461 tggctcgcgc tggcattcaa ccgatcccgg gacgcctggc gttcttcccg aatcgtcgcg
    20521 cggcgatggc ggatttataa gtacgagatt gaccagtcag cgcaaactga ctggtcagca
    20581 aactgcatta tttgttagct atgacggcga ttatcgctac ggcggcaacg tttgtcatag
    20641 tggcgaaccc accagtaacg gtcgcacacc tgttcatgcc cgccaacacg ggcacccgcc
    20701 agccagagaa tggcaccgac gaaaatactt agcactgcac catgtgcgaa atactggggc
    20761 atattaaact gtggtaactg gttgaggatt gaatacccca cgccgaccac cattaccacc
    20821 agacccaacc ccatgagcac gttaccgagt aacgaagcgt ttttgcgttt catatgtcac
    20881 ctccggaact ttctgggttg taacagggaa tacccctctt ccttatgtgt aaagtataga
    20941 caacaccctg tgggttatgt gcgggcgtga tcacaattac aacccttatt tcaacaaaac
    21001 tttacaaata aacgcctgaa caccttgact ttctaatagt ccactcatgc aattattacg
    21061 ttatctgcat ttgatacgca gttttttgtc aagcggggcc gcaaccagta aactacgcgc
    21121 cagttatgta cacactcagg acaaaaaaac gtgacgatta aattgattgt cggcctggcg
    21181 aaccccggtg ctgaatacgc cgcaacgcga cataatgctg gtgcctggtt cgttgactta
    21241 ctggcagagc gtttgcgcgc tccgctgcgc gaagaggcta aattctttgg ttatacttcg
    21301 cgagtcactc ttggaggcga agatgtccgc ctgttagtcc cgactacatt tatgaatctc
    21361 agcggcaaag ccgttgcggc gatggccagt tttttccgca ttaatccgga cgaaattctg
    21421 gtggcccacg acgaactgga tctgcctcct ggcgtcgcca aatttaaatt gggcggtggc
    21481 catggtggtc acaatggact gaaagacatc atcagtaaat tgggtaataa ccctaacttt
    21541 caccgtttac gcatcggaat cggtcatccg ggcgataaaa ataaagttgt cggttttgtg
    21601 ttaggcaaac cgcctgttag tgaacagaag ttaattgatg aagccatcga cgaagcggcg
    21661 cgttgtactg aaatgtggtt tacagatggc ttgaccaaag caacgaaccg attgcacgcc
    21721 tttaaagcgc aataagtcgt tgtctgcggc atttttgccg agtgccgtgt ataataggca
    21781 aagttatttc catttctgca atctgttagc aataacaggt tgattattaa gatattaagg
    21841 tgatttaaat catgggattc aaatgcggta tcgtcggttt gcccaacgtc gggaaatcta
    21901 ccctgttcaa cgcgctgacc aaagccggta ttgaagcggc caactttcca ttctgcacca
    21961 ttgagccgaa cacaggcgtc gtaccaatgc ctgaccctcg cctggatcaa ctggctgaaa
    22021 tcgtaaaacc gcaacgtacg cttcccacga ccatggaatt tgtcgatatc gccggtctgg
    22081 taaaaggcgc atcgaaaggc gaaggtctgg gtaaccagtt cctgaccaac atccgtgaaa
    22141 ccgaagcgat cggtcacgtt gttcgctgct ttgaaaatga caacatcatt cacgtttccg
    22201 gcaaagttaa cccggctgac gatattgaag ttatcaacac cgaactggcg ctggcagacc
    22261 tcgacacctg cgaacgtgcg attcatcgcg tacagaagaa agccaaaggt ggcgataaag
    22321 acgcgaaagc tgagctggcg gtcctggaaa aatgcctgcc ccagttggaa aatgcaggta
    22381 tgctgcgcgc gctggattta agcgctgaag agaaagcggc tattcgttac ctgagcttcc
    22441 tgacgctgaa accaacaatg tacatcgcca acgtcaacga agacggtttt gaaaacaacc
    22501 catatcttga ccaggtgcgt gaaatcgcgg cgaaagaagg ttctgttgtg gttccggttt
    22561 gtgctgctgt tgaagcagac attgccgaac tggacgacga agaacgtgac gagtttatgc
    22621 aggagcttgg gctggaagag ccgggcctga accgtgtgat ccgtgccggt tataagctgc
    22681 tgaacctgca aacttacttc accgctgggg tgaaagaagt gcgtgcatgg accattccgg
    22741 ttggagcaac cgcgccgcag gcagcgggca aaatccatac tgattttgaa aaaggcttta
    22801 tccgtgcaca aaccatctcg tttgaagatt tcatcactta caaaggtgaa caaggcgcga
    22861 aagaagcagg caaaatgcgt gcagaaggta aagattacat cgttaaagat ggcgatgtga
    22921 tgaacttcct tttcaacgtc taattaattt gttgtctcat gaggtttcgc taaatctcat
    22981 gaagagcgca aaagtatata taatccacgc aattgcgtgg atttttcatt tcaccgagtc
    23041 ctaatcattc ttgtaacatc ctacactttt gtgcttattg cctacagtga accacgagaa
    23101 taattaaaga catcagtgtc agatgacgga tatctcatta gtagtgatat aacgtaagtt
    23161 ttggtccact acacatcacc cccttcacag ctgatcaata ttgatcacgc tccaatatgc
    23221 tggcgagaaa aggacttacc tgaagggtaa tatcccgcag ataaagtccc cgaataacgt
    23281 gattacgtgc gatcctggca agatgaaaat gataaccagt agcacaataa caacgatagg
    23341 ccagaaaacc cattgaccaa aaatgaatat cgatgttttt tgctttttct tattagtaat
    23401 gcaaaaatgg cgcatattgt ttttgttatg cagatacatc aagcgaaatt cccataacta
    23461 attaggatta ttacaaaaaa tcttcaccaa attttttaac cgctgtttgt tgtaatattt
    23521 ttgccatatt tatagtaacg aattggaata agcaacgaca acttagggaa taactcgata
    23581 taaacccacc tttgtaaggg ggatgttaaa tatatattaa ttcctgtgtt cctgattttg
    23641 cccggtaata gagaattatt ctctgagaag ctcatcatga ttgcaccata caaatatcat
    23701 gatgataaga ggatattatt cattgattta aacaaaatga caaaatccat agacccatag
    23761 tctgggtact atataggagt ggtatgggca tcaaacaaca caatgggaat accaaagcag
    23821 atcgtctcgc tgaattaaaa atccgttcgc cctcaattca actgataaaa tttggcgcta
    23881 ttggtttgaa tgcaattatc ttttcccccc tgctgatagc tgctgataca ggaagtcaat
    23941 atggcaccaa tattactatt aatgatggtg acagaattac aggagatacc gccgatccat
    24001 caggaaacct ctatggtgta atgaccccag caggaaacac gcctggcaat atcaacctgg
    24061 gtaatgatgt caccgtcaat gtcaacgacg cctctggata tgcaaaagga atcattattc
    24121 agggcaaaaa cagctccctg acagctaacc gactcacagt agatgttgtt ggtcaaacct
    24181 ctgccatcgg cattaactta attggtgact atacccatgc tgacttaggc acaggcagca
    24241 ccattaagag taacgatgac ggcatcatta ttgggcatag ctcaacacta acagccactc
    24301 aattcaccat tgaaaactcg aacggtatag gcctaaccat caatgactat ggcaccagtg
    24361 tcgatcttgg aagcggaagt aaaatcacga ccgatggaag tacaggtgtt tatatcggtg
    24421 gtctcaacgg caataacgcc aatggtgctg cgcgttttac ggcgacagac ctgacaatcg
    24481 atgttcaggg ctacagcgcc atggggataa acgtacagaa aaactctgtt gtcgatctcg
    24541 gaacaaacag taccattaaa accaatggcg ataatgcaca cggcctctgg agctttggcc
    24601 aggttagcgc gaatgcactc actgttgatg taactggagc cgcggccaat ggcgtcgaag
    24661 ttcgtggtgg tacaaccact atcggtgcag atagccatat ttcttccgcg cagggcggtg
    24721 gcctcgtcac cagtggttca gacgcgacaa tcaatttttc tggcacggca gcgcaacgaa
    24781 acagcatctt ttccggcggt tcttatggtg cctcggccca gacggcaacg gctgttgtca
    24841 acatgcaaaa taccgatatt acagttgatc gtaatggcag tctggcgctg ggtttgtggg
    24901 cgctcagcgg cggtagaata accggagaca gtttggctat caccggcgcg gcaggagcca
    24961 ggggaattta tgccatgacc aacagccaga tcgacctcac gagcgatctg gtcattgata
    25021 tgagtacacc cgaccagatg gccattgcaa cgcaacatga cgatggttat gccgccagcc
    25081 gcatcaacgc ctcgggtcgt atgcttatca acggtagcgt tctttccaaa ggtgggctaa
    25141 tcaatctaga tatgcaccca gggtcggttt ggacaggttc ctccctcagc gataatgtca
    25201 atggcgggaa actggacgtt gcaatgaata acagcgtctg gaacgtaaca agtaattcta
    25261 atctcgacac gctggcgctg agccattcaa ctgtcgattt tgccagccac gggtcaactg
    25321 ccggcacatt tgccacatta aacgtagaga acctgagcgg taacagtacc tttattatgc
    25381 gtgctgatgt tgttggcgag ggtaatggcg ttaataataa aggggattta ttgaatatca
    25441 gcggaagtag tgctggtaat cacgtattgg ctatccgcaa ccagggcagc gaggccacta
    25501 cgggaaatga agttctgaca gtggtaaaaa ccactgacgg tgcggcctcg ttcagcgcgt
    25561 cttctcaggt tgagttgggg ggatatctgt acgatgtgcg taaaaatggc actaactggg
    25621 agctttacgc ttccgggaca gttccggaac cgactcctaa tcctgaacca acaccggctc
    25681 ccgctcagcc tcccatagtc aaccccgatc ctacgcctga acccgatcct acgcctaacc
    25741 ccactcccac acctaaaccc accacgaccg cagatgctgg cggcaattat ctcaatgtcg
    25801 gttacttatt aaactatgtt gaaaaccgta cgctgatgca acgaatgggt gacctgcgaa
    25861 atcagagtaa agacggtaat atctggctgc gcagttatgg gggaagcctg gactccttcg
    25921 ccagtggcaa actgagcggc tttgacatgg gttacagcgg tatccagttt ggtggggata
    25981 aacgtctctc tgatgtaatg ccgttgtatg tcggtctgta tattggctca acacatgcat
    26041 cgccggacta tagcggaggc gacggtaccg cacgttcaga ctacatggga atgtatgcca
    26101 gttacatggc acacaacggt ttttacagcg atctcgttgt aaaagcatcg cgccagaaaa
    26161 atagttttca cgtactggac agtcagaaca acggcgttaa cgccaacggc actgcgaatg
    26221 gattgagcat ctccctggaa gccgggcaga ggttcaacct gacccctact ggttatgggt
    26281 tctatataga gccgcaaacc cagctcacat acagccacca gaatgagatg gctatgaagg
    26341 cgagtaatgg tctcaatata catctgaatc actacgaatc gctgctgggg cgtgccagca
    26401 tgatactggg atatgacatc accgcaggca acagccagct gaatatgtat gtgaagactg
    26461 gagctatccg cgagttttca ggggatacag actatctgtt gaacaactcc cgggagaagt
    26521 acagtttcaa aggtaatggc tggaataacg gcgtgggagt cagtgcacag tataacaaac
    26581 agcacacatt ctacctcgaa gcggattaca cgcagagtaa cctctttgat cagaagcaag
    26641 tcaacggagg atatcgcttc agcttttaag tgcgggatag accagccctt aaacgatgct
    26701 gaaaaatgtt agcgtgcgcc ggaatgaaat atagatcact ctgatgtatc agaaatcgtc
    26761 tctgtctttc atacccggcg cttaaactgc cctgcatcaa tgccatgttg cttcattttc
    26821 cgccacagcg tagtgcggcc gatcccaagt aaagccgaca tttcctgaat gcgaccgcct
    26881 gtgacctggg ctgcgttaat aattgcctct ttttcaactt ccgcaaatga cagactggtg
    26941 gaaaggcgtg tggcgctgac atcatctgtt gcctgctcgg taaacagatg ttccggcaaa
    27001 tcactgacgc gaatgcgccc attatcacta ctcagagcaa gattctcgat gacgctgtaa
    27061 agttcaaaat cgttgccagg ccatgcacag gaaaccaggc gagcgagggc atcgtcatca
    27121 attttcagcc gcgtagagaa gcgtttttca agactgcgta atttgttatt caccagcgcc
    27181 ggaatgctgc cacgccgcat acgcagaggc gggatggtaa tttcaaatgc atgcaacgcg
    27241 taatacagct ggcgactaaa acgattttgt tccaccagca ttgcgaggtc cgcggtcgtt
    27301 gtggcaatca ctttgacatc aattggtatt aagcgccgcg catccagtcg cgtgataaca
    27361 ccctgcttga taacctgaag caaagcagac tgtaactcca ccgccagata ttcaatcttt
    27421 tcaagaaaca gcgtgccgcc gtgcgccagt tccagccgac tcagacggcc attttcattg
    27481 tccgtgcgat cgccaccaat aaattcttcc gccagtgccg catcaccata taactcacaa
    27541 ttgacggcga tataaggacc tgcagcacgc tcgctttcat tatgaattgc ctgacttagc
    27601 agtgccttgc ccacgccctc ttctccacaa agcaggacag gaaagctact gcgcgccgcc
    27661 tggcgaccaa aatgaatcaa gcggcgggtt tgcggatcgt cctgtggcat atgagcgaag
    27721 gtatggctga cttttcctaa ttgactggtc atcaactgcc gcatctgttc cacaggatgg
    27781 agcaacaaaa taaaactggt tccctgcgtt tctattatcg gtttaagggt tatcaccgca
    27841 tcaataaact ggtgctgact ttcaaaggtt gcttctacgt gtttgagcgg atgtgcctgt
    27901 tttattgctt gttgcaatac ggcgggtaac gttaagagtt cagtgattgc ccttccctga
    27961 cttgccgtcg cgtcaaggcg caagacccgc gccgcctggg cattaataaa ttgcaaatta
    28021 ccctgcacgt cccagctaat cacgccatca tccatacttt ctaacagggc attaagttga
    28081 tttaaatgac ggttagtttc agcgagcaaa ctgtccgtca gcagtaaatt tccgacctcg
    28141 cgggcgattg ccaacgtcaa cggcaaatca gctgcggtag tttgctcaac cggacacgcc
    28201 agcgctattg ttcccgtcaa tcggcccttg ctgtcaaaca acggcgttgc acaaaaggcc
    28261 cagttccaga gtgcctgttt gaaatgttga tcggccatcg ttttcacggc ctgaccagag
    28321 atagccgcta acgatagcgc acaagtacca ataattccct cggcgcaata cgtgccgtca
    28381 ttgaatccca gtgcacttag ctgctgcaag gtttgcggat cgccattacg gctgagaatg
    28441 caggcggttt catcgaggat aaacagcgca cactctcgcg gtgacatata ttcccaggca
    28501 tcttccagcg cagcctgccc gagcgtcagc atcgctttct tacgccgata aatagaagca
    28561 aatgtcacgc cctgggcctg atgtggtacg ttccatgtct cccgtttcat cagcttattg
    28621 catcgctccc aggaggttgc aattaaggga gatatgcccc gaccatcgtt gttaaaagcg
    28681 ccactcatat cccgcatccc ttatgacaaa aggtgttccg gctcgcaatt ttaagtatta
    28741 atacacgtaa aagtttcaat atggaacatt cattaaagca tatgtttcac tatgaaacat
    28801 aatttgggct aattttcttt tcccttgcct gatgcacaat ggattcatcg gcagtcagtg
    28861 gtcgccgtgt cgttgaacat catccatgcc ctaccgtaat tgctggagca aaataatgaa
    28921 aaaattgatc aatgatgtgc aagacgtact ggacgaacaa ctggcaggac tggcgaaagc
    28981 gcatccatcg ctgacactgc atcaggatcc ggtgtatgtc acccgagctg atgcccctgt
    29041 tgcaggaaaa gtcgccctgc tgtcgggtgg cggcagcgga cacgagccga tgcactgtgg
    29101 ttatatcggt caggggatgc tttcgggggc ctgtccgggc gaaattttca cctcaccgac
    29161 gcccgataaa atctttgaat gctccatgca aattgatggc ggcgaaggtg tactgttgat
    29221 tatcaaaaat tacaccggcg atattcttaa ctttgaaaca gcgaccgagt tactgcacga
    29281 tagcggcgta aaagtgacca ctgtggtcat tgatgacgac gttgcggtaa aagacagtct
    29341 ttataccgcc gggcgacgcg gcgttgccaa caccgtatta attgaaaaac tcgtaggcgc
    29401 agcggcggag cgtggcgact cactggacgc ctgtgcggaa ctggggcgta agttgaataa
    29461 tcaaggccac tcaataggta tcgctctcgg tgcctgtacc gttcctgccg cgggcaaacc
    29521 ttcttttacc ctggcggata atgagatgga gtttggcgtc ggcattcatg gtgagccggg
    29581 tattgaccgc cgctccttct cttcccttga tcaaaccgtc gatgaaatgt tcgacaccct
    29641 gctggaaaat ggctcatacc atcgcacttt gtgtttctgg gattatcaac aaggcagttg
    29701 gcaggaagaa caacaaacca aacaaccgct ccagtctggc gatcgggtga ttgcgctggt
    29761 taacaatctt ggcgcaactc cgctttctga gctgtacggc gtctataacc gcctgaccac
    29821 acgttgccag caagcgggat tgactatcga acgtaattta attggcgcgt actgcacctc
    29881 actggatatg accggtttct caatcacctt actgaaagtt gatgacgaaa cgctggcact
    29941 ctgggacgcc ccggtccaca ccccggccct taactggggt aaataaggag aaagcaatgt
    30001 cactgagcag aactcaaatt gttaactggc tcactcgttg tggcgatatt ttcagcaccg
    30061 agagcgagta tcttaccgga ctggatcgcg aaattggcga cgctgaccac gggctaaata
    30121 tgaaccgagg ctttagcaaa gtggtggaaa aactccctgc tatcgcagat aaagatatcg
    30181 gtttcattct caagaatacc ggtatgacgc tgctttccag cgtcggtggt gccagtggtc
    30241 cgctgttcgg taccttcttt atccgcgccg cacaggcgac ccaggcacgg caaagcctga
    30301 cactggaaga gctttatcag atgttccgcg atggcgtgga cggcgtaatc agtcgcggga
    30361 aagccgaacc tggcgataaa accatgtgtg atgtgtgggt gccggtggtg gaatcgttac
    30421 gtcagtccag cgagcaaaat ctctctgttc cggtggcgct cgaagctgcc agtagaatcg
    30481 ccgaatccgc tgcacaaagt acgattacga tgcaagcccg caaaggccgc gccagttatc
    30541 tcggtgaacg cagtattggt caccaagatc ccggcgcgac ctcggtgatg tttatgatgc
    30601 aaatgttggc gttagccgca aaagagtaag gaattggtga tggtaaacct ggtcatagtt
    30661 tcacatagca gccgactggg agaaggtgtc ggtgaattag cccgtcagat gttaatgagt
    30721 gatagttgta aaatcgccat tgccgcggga attgacgatc cacaaaatcc tattggtacc
    30781 gatgccgtca aagtgatgga ggccatcgaa tctgttgctg atgccgacca tgtgctggtc
    30841 atgatggata tgggtagcgc attattgagt gctgaaactg cgctggaatt gctggctccc
    30901 aagatcgccg caaaagtacg tttgtgtgct gcgccgttgg tcgaaggtac actggcagca
    30961 acggtcagcg cggcctcggg agcggatatc gacaaagtta tctttgacgc tatgcatgcg
    31021 ctggaagcca aacgtgaaca actgggttta ccgtcctccg acactaaaat ctctgacaca
    31081 tgtcctgcgt acgatgaaga agcccgttct ctggcggtgg tcataaaaaa ccgtaacggc
    31141 ctgcatgtac gtccggcctc ccggctggtt tataccttat cgacatttaa tgccgatatg
    31201 ttgctggaaa aaaacggcaa atgcgtcaca ccagagagta ttaaccagat tgcgttacta
    31261 caagttcgct ataacgatac gctgcgcctg attgcgaaag ggccagaagc tgaagaggca
    31321 ctgatcgctt tccgtcagct ggctgaagat aactttggtg aaacggagga agtcgctcca
    31381 cctactctgc gtcccgttcc gcctgtttcg ggtaaagcct tttattatca accagtttta
    31441 tgtacggtac aggcaaaatc aaccctgacc gtggaagaag aacaagatcg attacgccag
    31501 gctattgact tcacgttatt agatctgatg acgttaacag caaaagcaga agccagcggg
    31561 cttgacgata ttgccgcaat cttttctggt caccatacac tgttagatga tccggaactg
    31621 ctggcggcgg caagcgaact ccttcagcat gaacattgca cggcagaata tgcctggcag
    31681 caagttctta aagaacttag ccagcaatac cagcaactgg atgatgaata tctacaagct
    31741 cgctatattg atgtggacga tcttctgcat cgcaccctgg tccacctgac ccaaacgaaa
    31801 gaagaactcc cgcagtttaa ctcgccaact attctactgg cggagaacat ttatccttcc
    31861 acagtactgc aactggatcc ggcggttgta aaaggtatct gccttagcgc cggaagtccg
    31921 gtatcccaca gcgccctaat cgcccgtgaa ctggggattg gctggatttg ccagcagggt
    31981 gagaaactgt atgcgataca accagaagaa acgctaacgc tggacgttaa aacgcaacgt
    32041 ttcaaccgtc agggttaagc caccgttcgg atggcatcgt tctgatgtca tccggcaatt
    32101 tacccagcac tgatgttttt taactacctg ttatattttt taccaattta tgatgatttt
    32161 tcttcgctaa tcgcgaaccc aacgtcgctg actggctaat aaacagacgt tatttgatca
    32221 ctctatttca ccgttattta tccctttaat tcccttttct aaaatgcctg acagttcgca
    32281 gaatgagatt tcgatcatgc agctagtgcg atcctgaact aaggttttct gatacttgaa
    32341 taccgtttta ttcggtttcg ccaaaggaga atgattgatg aaatcccccg caccttctcg
    32401 cccgcaaaaa atggcgttaa ttccagcctg tatctttttg tgtttcgctg cgctatcggt
    32461 gcaggcagaa gaaacaccgg taacaccaca gccgcctgat attttattag ggccgctgtt
    32521 taatgatgtg caaaacgcca aactttttcc ggaccaaaaa acctttgccg atgccgtgcc
    32581 gaacagcgat ccgctgatga tccttgctga ttatcggatg cagcaaaacc agagcggatt
    32641 tgatctgcgc catttcgtta acgtcaattt caccctgccg aaagagggcg agaaatatgt
    32701 tccgccagag gggcagtcac tgcgcgaaca tattgacgga ctttggccgg tattaacgcg
    32761 ttctaccgaa aacaccgaaa aatgggattc tctgttaccg ctgccggaac cttatgtcgt
    32821 gccgggcgga cgttttcgcg aggtatatta ctgggacagt tacttcacca tgttaggact
    32881 tgccgaaagc ggtcactggg ataaagtcgc ggatatggtg gccaattttg ctcatgaaat
    32941 agacacttac ggtcatattc ccaacggcaa ccgcagttac tatttaagcc gctcgcaacc
    33001 gcccttcttt gccctgatgg tagagttact ggcgcagcat gaaggcgatg ccgcgttgaa
    33061 gcaatacctg ccgcaaatgc aaaaagaata tgcttactgg atggacggtg ttgaaaacct
    33121 gcaagccgga caacaggaaa aacgcgttgt aaaacttcag gatggtaccc ttctcaaccg
    33181 ctactgggac gatcgcgata cgccacgacc agagtcatgg gtggaagata ttgccaccgc
    33241 caaaagcaat ccgaatcgac ctgccactga aatttaccgc gacctgcgct ctgccgctgc
    33301 gtctggctgg gatttcagct cgcgctggat ggacaacccg cagcagttaa ataccttacg
    33361 caccaccagc atcgtaccgg tcgatctgaa cagcctgatg tttaaaatgg aaaaaatcct
    33421 cgcccgcgcc agcaaagctg ccggagataa cgcgatggca aaccagtacg aaacgctggc
    33481 aaatgcccgt caaaaaggga tcgaaaaata cctgtggaac gatcaacaag gctggtatgc
    33541 cgattacgac ctgaaaagtc ataaagtgcg caatcagtta accgcggccg ccctgttccc
    33601 gctgtacgtc aatgcggcag cgaaagatcg cgccaacaaa atggcgacgg cgacgaaaac
    33661 acatctgctg caacccggcg gcctgaacac cacgtcggtg aaaagtgggc aacaatggga
    33721 tgcgccaaat ggctgggcac cgttacagtg ggtcgcgaca gaaggattac aaaactacgg
    33781 gcaaaaagag gtggcgatgg acattagctg gcacttcctg accaatgttc agcacaccta
    33841 tgaccgggag aaaaagctgg tggaaaaata tgatgtcagc accaccggaa cggggggcgg
    33901 cggtggcgaa tatccattac aggatggctt tggctggacc aatggcgtga cgctgaaaat
    33961 gctggatttg atctgcccga aagagcaacc gtgtgacaat gttccggcga cgcgtccgac
    34021 cgttaagtca gcaacgacgc aaccctcaac caaagaggca caacccacac cttaaccagc
    34081 gcttactccg acaacgtcat tcggcttctc cggatggcgt tgtcggcaga taacaccata
    34141 gctatggttt tttctacaca gacggttttt caagtcctgg cgggaagtat tgcgcaaatg
    34201 tttgccatac gtggttgccg aagatagcgt catttctacg ggtaagtgtt tcactttcat
    34261 cacccggcga tttacgccga cgttggctat ataaagcatc aattgctacc tgaatattgt
    34321 tacggtgttc actcatcaac gtttttttgc tttctggagt taaattaata atcacataat
    34381 ccagacactc caaagaattt agcaaccaaa aaaagaattc agaccattcc tcttcgcgta
    34441 actgaagcat tgccgaacgg gtattttggc ttaatctgcc agaaggattg atcaattcta
    34501 aatgttcaat aagcgcagcc aaaaccgcct cactatccgc cccattttta acgcctgact
    34561 ggctactgtc taaaatattt attttctgaa tcacaccaaa aacaacgaaa caataattaa
    34621 aaaaggattc tgttgttgtt accgggaacc gactttatcg gatatcgaag tgctatattg
    34681 atttttttca cccgtaaagc aggtgtcgag tatacgttct gatgtgaaag cggatagcgg
    34741 catctgtatc gctatccgcc ttatgaaaat cttattctct tcgcaggagg cggaatatgc
    34801 ccagaacgag aatagctccc accaccgcca ccaggaagct gtgcagatta aaaccactga
    34861 tggagccgcc aatgccaaac atggtcgcca gccagccgcc gaccaccgca ccgactatcc
    34921 cgagaataca ggtcaggaaa aatccaccac catcacgccc cggcatgatt agcttggcga
    34981 taatgccggc tatcaggcca aaaataatcc aggcaataat tcccatgttc aggccctcgt
    35041 ttcgatgaat cggacgcaag aaatgcttac atcaacgctc taagtatagg caagggcggg
    35101 atcgatgctg ggcgtatgtc ggctgattta agaacagcat gtaaaaaata ttcacacatt
    35161 tgtattaagt tttgttaact gtgaccgata aaccaaagac agtttgtcag tcaggagttt
    35221 ttccgcgtga gtcattacca tgagcagttc ctgaaacaaa atccgttagc cgtcctgggc
    35281 gtgttacgcg atttgcacaa agccgcaatt cctttgcggc tcagttggaa tggcgggcag
    35341 ctgatcagca aaatactggc aataaccccg gataaactgg tgctggattt cggcagtcaa
    35401 gccgaagaca acatcgccgt gctaaaggcg cagcacatta ccattaccgc cgaaactcag
    35461 ggtgcgaaag tcgagtttac tgttgaacaa ctacagcaga gtgaatactt gcagcttccg
    35521 gcatttatta ccgtaccgcc tcccacctta tggtttgtac aacgacgccg atatttccgc
    35581 atctccgccc cactccatcc gccttatttt tgccagacca aactggcgga taacagtacg
    35641 ttacgtttcc gcctgtatga tttgtcgtta ggcggcatgg gcgcattact ggaaacagca
    35701 aagcctgccg aattacaaga aggcatgcgc ttcgctcaga ttgaagtcaa catggggcaa
    35761 tggggtgttt ttcactttga cgcccagtta atctccatca gcgagcgcaa agtgattgat
    35821 ggcaagaatg aaaccatcac cactccccgt ctgagcttcc gttttcttaa cgtcagcccg
    35881 acggtggagc ggcaattaca acggattatt ttctctctcg agcgggaagc ccgggagaaa
    35941 gcggacaaag tacgcgactg atttacatcg cgtccagtgc ctgctcaagt ttgtagatat
    36001 agcgcggagc ctgcggcgca gggtgatttc gcgctacgtg ttcgaggaac tcgtcagcat
    36061 ccaaatcgtt gattttgctg atcgcctttt tccgatctga cgagaaagtc cgtagcagcg
    36121 cacctgcccc gttagcgtat gacaccacca gcgcatattg cagtaccttc ggatcttcaa
    36181 tgcctgccag cgggccggtt tccagaatat tcaggtaagc cgcccccatt gaaatattac
    36241 gctctggatt tttcagttcg ctggtcgtcg gctcaccact ccagcccata cggcgataaa
    36301 catcacgtcc ggaggttgaa gcttttaact gcatcaaacc aatggcattc gatttactca
    36361 ccgcgttcgg attaccaccc gattcgatag cgataatcgc cgtgatcaat tgtggatcga
    36421 cgccccaggc tgcaccggct ttctggctta ttggcatcca ctgcatcgca cgttgcaccg
    36481 gaactttcgc gttccacggc gggttcgtat agtcatgctt tgatgaacaa cccgctaata
    36541 acacaatcaa aaaggcaaac catctcaatt tcacttcttc tatccttata gcctggaatc
    36601 gccgttgagg tgacaacggc acacctaagc ggcatgatat acatttcgtt tctgttgtca
    36661 gcaaggaatt gccatgtctc tgtttcactt aattgcccca tcgggttact gcattaaaca
    36721 gcacgccgcg ctgcgtggta tccagcgcct taccgacgag gggcatcagg tcaataacgt
    36781 cgaggttatt gcccgtcgtt gtgagcgttt tgcgggtacg gaaacggagc gtctggaaga
    36841 tctcaattcc cttgccagac tgactacccc caacaccatc gtgctggctg tacgcggcgg
    36901 ttacggtgcc agtcgtttac tggcagatat tgactggcag gcactggtgg ctcgccaaca
    36961 acatgacccg ttgctcattt gcgggcatag cgattttacc gccattcagt gcggtcttct
    37021 ggcgcaaggc aatgtcatca cctttagcgg ccccatgctg gtagcaaact ttggcgcgga
    37081 tgaactgaac gcctttaccg agcaccattt ctggctggcg ttacgcaata aaaccttcac
    37141 cattgaatgg caaggggaag gcccgacatg tcagaccgaa ggcacgttgt ggggaggcaa
    37201 tcttgcgatg ctgatttcac tgattggtac accgtggatg ccaaaaattg agaacggtat
    37261 tctggtgctg gaagatatta acgagcaccc tttccgggtc gaacgtatgc tgttgcagct
    37321 ctatcatgcg ggaattttgc cacgtcagaa ggcgatcatt ctcggtagct ttagcggcag
    37381 cacgcccaat gattatgacg caggttacaa cctggagtca gtgtacgcgt ttttgcgttc
    37441 ccgcctgtcg attccactca ttaccggcct cgattttggt catgaacaac gcacggttac
    37501 gctgccgttg ggcgcacatg ccattctgaa taacacccgg gaaggcactc agttgacgat
    37561 tagcggtcat cctgttctta aaatgtaaaa aaatcacgct tcagggctaa gtaaaacgct
    37621 gtctctgccg ctaatatggt agggtttata taagagacag cgtaatcagg agtaaccgac
    37681 cgttggatgc cacaacaata attagccttt tcatcttagg atccatcctt gtcaccagca
    37741 gtatattact tagttcattt tcttcccgtc ttggcattcc tattctggtt atttttctgg
    37801 cgatcggcat gctggcagga gtcgatggcg tcggcggtat cccgtttgat aattacccct
    37861 tcgcctacat ggtaagtaac ctggcactgg cgattatttt gctcgacggc gggatgcgca
    37921 ctcaggccag ttcctttcgt gtggcgttag gaccggcact gtcgctggcg acgctgggcg
    37981 tgcttatcac ctctggttta accggcatga tggcggcgtg gctgtttaat cttgatttga
    38041 ttgaaggctt attaatcggc gctatcgtcg gctctaccga tgctgcagcg gtcttttctc
    38101 tgctgggtgg taaggggctt aacgaacgtg ttggctcgac gctggaaatt gaatccggca
    38161 gtaatgatcc aatggcggtc tttctgacga ttaccctgat tgcgatgatc cagcaacatg
    38221 aaagcagtgt cagttggatg ttcgtggtcg atattctgca gcaatttggc ctcgggattg
    38281 tcatcggact tggcggtggt tatttactgc tgcaaatgat taatcgcatc gccctgcctg
    38341 ccggattata tccattgctg gcattaagcg gcggtatttt aatttttgct ttaaccacgg
    38401 cgctggaagg cagcggtatt ctggctgttt atctgtgcgg ttttctgctg ggtaatcggc
    38461 cgattcgcaa ccgctacggc atcctgcaaa atttcgacgg cctcgcctgg ctggcgcaaa
    38521 tcgccatgtt cctggtgctg gggctattgg ttaacccaag cgatctgctg cctattgcca
    38581 ttccggcgct cattttgtcg gcatggatga tattcttcgc ccgtcccctt tcggtatttg
    38641 ctggattgct ccccttccgc ggtttcaatc tgcgtgagcg cgtgtttatc agctgggtag
    38701 gattacgcgg cgcggtgcca atcatcctgg ccgtgttccc gatgatggcg gggctggaga
    38761 atgcacgtct gttctttaat gtcgccttct ttgtggttct ggtttcactg ctcttgcagg
    38821 gaacatcact ctcgtgggcg gctaaaaaag ccaaagtggt ggttccgcca gtgggacgtc
    38881 cggtgtcacg cgttggcctg gatattcatc cggaaaatcc gtgggagcag tttgtttatc
    38941 aattgagtgc cgataaatgg tgcgtgggcg cggcactgcg cgatttgcat atgccaaaag
    39001 agacgcgtat tgcggcactg tttcgtgata accagttgct tcatcctacc ggcagcaccc
    39061 gactgcgcga aggcgatgtg ttgtgtgtaa ttggtcggga acgcgatctt ccggcgctcg
    39121 gtaaactgtt cagccagtcg ccgccggtcg cgctggatca acgcttcttt ggtgacttca
    39181 ttctcgaagc cagcgccaaa tatgctgatg ttgcgctgat atatggcctg gaagacgggc
    39241 gagaatatcg tgataagcag caaacgctgg gtgaaattgt ccagcagttg ttaggcgcgg
    39301 caccggttgt cggtgaccag gtagagtttg ccgggatgat ctggacggtg gccgagaaag
    39361 aagataatga agtgttgaag attggcgtac gggtggctga agaggaagct gaatcttaaa
    39421 tttaatgaag catctatcgc cggttgcgat gctttgctga acgcagcctg agcctgcaat
    39481 tgtttgtcgt ccataattta accttcattt gatgttgaat tgaacatatc aaaatcaggc
    39541 aattacacaa atctatgtac aggctccatc aagggtaaag cgtgatttat ctgaagtcga
    39601 ctccgagtca acaatgaagc gtaagcgttt tatgtagaac gcctgtgcgg tgagttcagg
    39661 ttccggggaa atagtgtcca tccttttaat ggacactatc atgggcaatg gctctgtcac
    39721 attaacaact acagttgctg accagccgga tggatgattt tttcgcaacc agaagacgct
    39781 gcctccgatc cggcttacaa caagttacac tgtcacaacc gggacgcgta acgccagcgc
    39841 gcacatcaac tcatagccca ccgttccggc agcggcggcg acatcatcaa ttttgatctc
    39901 cttgccccac agctcaaccg gcgtaccaat acccgcctgc gggcaaggcg ttaaatcgac
    39961 cgccagcata tccatcgaga cggtccccac cgtcatggtg cgcacgccgt ccactaaaac
    40021 aggggtaccg gtaggcgcgt ggcgcggata accgtcggcg taccctgcgg cgacaatgcc
    40081 gattcgctgt tcatcgcgcg cagtatagcg accgccgtag cccacacgct cacccgcttt
    40141 tagcgtctgg acaccaataa tctcactgct tagagtcatc accggacgta atccggtatt
    40201 ggcgatatca cgccactgac cggacggcga agcgccatac aaaataatgc caggccgaac
    40261 ccagtcaaaa tgcgcttccg ggtgccacag agtcgccgcc gaattggaca acgaacgccg
    40321 acactccagc ccttccgccg cctgctcaat acgcgccatc gcgctggaaa ttccatcagg
    40381 atgttccgct tcggcaaaat gcgacatcag ggtcatctcg ccaacattcg ccattgcccg
    40441 caactgctgc cagacggtaa gcacgcgatc gggctggaag cccaaccgat tcatcccact
    40501 gttcacttta agataaatat ccaacggtgc ttttagccgc gcattttgca gtgctttgag
    40561 ctgccagttg ctgtgtacgc aggtggtcag gcggtgctgg tcataaatct ccagatcctg
    40621 agcatggaaa aatccttcca gcatcaggat cggccccttc cagccgcgct cacgtaacgt
    40681 tattgcctct tccaggttaa gtaatgcaaa gccatcggtg gccccgagcg cgctccagat
    40741 acgctcaata ccatgcccgt aagcgttcgc ttttaccacc gaccagacgc gcgcgtgcgg
    40801 cgcggcctgg cggacaatgg acagattctg ttttaatgcc tgcagatcga ggctggcctg
    40861 tatcggacgg gtcatctcgt ttccttagct gtgtgcgcca tgtaaatggc ccggacgtga
    40921 tggcgtaaat ccacggctgt agcgcgctac gcttagatcc tcatatggga tcgctggcgt
    40981 gcgaccagag agcagatcgc ttaacaactg accggaaccg caagccatcg tccagccgag
    41041 cgtgccgtga ccggtattca gccacagatt tttaaagcgt gtacgcccga caaccggcgt
    41101 accgtctggc gtcatcgggc gcaaacccgt ccagaaagtc gcctgctcga catgaccgcc
    41161 gcgtggatag agatcgcgaa ccaccatttc cagcgtttca cgacgcggtt gcaacagctc
    41221 ggtgttaaaa ccaacaattt ccgccattcc gccaacacga atgcggttat cgaaacgggt
    41281 aatggcgatt ttgtaggttt catcaagaat agtggatact ggcgcaccat cttcctgcgc
    41341 aattggaata gtcagcgagt agcctttcag cggatacacc ggaatatcaa caatgccttt
    41401 gagcatcgcc gtcgagtaag aaccaaacgc catcacatac gcatcggcct taatcacttc
    41461 atcgccacac ttcacgccgt agatttgctc gccgtcgcaa agcagttggt caacgggcgt
    41521 attaaagcgg aatttaaccc ccgcctgctc tgccatccgc gccagattct gggtaaacag
    41581 ctggcagtct ccggtttcat cattgggcag ttgcagacca cccgtcagtt tgtgcgctac
    41641 ttctgccagc gcgggctcca cttccgccag gcggctggat tccagcagct gatacggtac
    41701 gccggcatct tccagcacgg cgatatcgcg ggtcgcattt tcatactgtt gttcggtacg
    41761 gaacagttgc agcgtcccac cctgacgccc ttcatactga atattggttt cggcgcgcaa
    41821 tgctttcagg caatcacggc tgtattccgc cagacgcacc atccgccctt tgttttccat
    41881 gtagtggctg gtgtcgcagt tacgtaacat ttgccacatc catttcaact gaaactgcgt
    41941 accgtcgaga cggaccgcca gcggcgcatg gcgctggaac atccatttga tcgcttttaa
    42001 aggcacacct ggtgccgccc acggtgcagc atatccgggg gagatttgcc cggcattcgc
    42061 ggcgctggtt tccagggcgg cccccggctc ccgatcaatg acggtgacct catgtcctgc
    42121 ctgatttaag taccaggcgc tggcaacgcc taccacacca cttcccagta tgacaactcg
    42181 catagccact ccgataacgg tagatacagt aaaagaataa taatctaatt acatcttgat
    42241 aactcagttg aaaatattat tcaacatacg gcttttttat ggtgagctgg ctcacatctc
    42301 cctgactctg cggcaatatg gcgtctttag tatctcctgc tgaacgccct ttttatccag
    42361 cataaaattc cgttcagaag cggattagtg gcactctgat aataagaaat cgcaaagaaa
    42421 aaaattctcc ggcgacgcgg tttttaacag gtgttctatg cttgaaatga ggtgctccac
    42481 acagagagcg ccgacaacaa tgaggtgcgc gtatggcgac gatcgattct atgaataagg
    42541 acaccacacg tttgagcgat ggacccgact ggacgttcga cctgctggat gtttatctgg
    42601 cagagataga ccgggtggcg aaactctacc ggctggatac ctacccgcac cagattgaag
    42661 tgataacctc agaacagatg atggatgcct actccagcgt cggcatgcca attaactatc
    42721 cgcactggtc attcggtaaa aagtttatcg agactgaacg gctgtataag cacggtcagc
    42781 aaggactggc ctatgaaatc gtcattaact ctaacccgtg tatcgcttac ctgatggaag
    42841 agaacaccat taccatgcag gcgctggtga tggcacacgc ctgctatggg cataactctt
    42901 tctttaaaaa caattactta ttccgcagtt ggaccgacgc cagttcgatt gtcgattatc
    42961 tgatttttgc ccgtaaatat attaccgagt gcgaagaacg ttatggcgtt gatgaagtag
    43021 aacggcttct ggactcgtgc cacgcgctga tgaactacgg cgtggaccgg tacaaacgcc
    43081 cgcaaaaaat ctcgctgcaa gaagagaaag cccggcagaa aagtcgcgaa gagtatctac
    43141 aaagtcaggt caatatgctc tggcgtaccc tgccgaagcg cgaggaagag aaaacggttg
    43201 ctgaagcgcg ccgctatccg tccgaaccac aagaaaacct gctctatttt atggagaaaa
    43261 atgcgccact gctggaatca tggcagcgtg aaatcctgcg tattgtgcgt aaggtgagcc
    43321 agtattttta tccgcaaaaa cagactcagg tgatgaacga aggctgggcg accttctggc
    43381 actataccat ccttaaccat ctgtatgatg aagggaaagt aacggaacgt tttatgctgg
    43441 agtttttgca cagccacacc aatgtggtct tccagccccc ctataacagc ccgtggtaca
    43501 gcggcatcaa cccgtatgcc ctcgggttcg ccatgttcca ggatattaaa cggatttgtc
    43561 agtcgccaac ggaagaagac aaatactggt tcccggatat cgccggttcc gactggctgg
    43621 aaacgctgca tttcgcgatg cgtgatttca aagatgagag ttttatcagc cagtttctgt
    43681 caccgaaagt gatgcgtgat ttccgcttct tcaccgtgct ggatgatgat cggcataatt
    43741 atctggagat ttccgctatt cataatgaag aaggttatcg ggagatccgt aaccggttat
    43801 cgtcgcaata taacttaagt aatctggagc cgaatattca gatctggaac gtggatttgc
    43861 gcggcgaccg ttcgctgacg ctgcgttaca ttccacataa tcgcgcaccg ctggatcggg
    43921 ggcgcaaaga agtgctgaag catgtgcatc gcctgtgggg atttgatgtg atgctcgaac
    43981 agcaaaacga agacggcagc atcgagttgc tggaacgttg cccgccaaga atgggaaatc
    44041 tgtaaaaaca acaaaaaacc cctcgtttga ggggtttgct ctttaaacgg aagggattat
    44101 cgcccctgaa tggctaaatc acccggcaga tttttctgca tccggtgcca aatctcgcca
    44161 ctctcatgcc catagcgacg cactgtttcg tacacctgat cgtgcgcgcc ttcactgcac
    44221 aacgccgaca gtttgtggta gaagcccagc gccagactgc gcgcttccgg attggcgaaa
    44281 tagtgacgac caatgcgcgt atacagccct ttcatcccgt taaggatcag accgtaaatc
    44341 gggttgccgg aagcaaacgc caggccgcgg aatatgttgt aatccagctc ggcaaaggca
    44401 tcggcgtgat cggccacttc attagcggta gccagcactt cctgcgcttt atcgggatgc
    44461 tgacgaaacg cggtgcgaat aaaaatagtg gaaatattgg tacgcaccga cagcaaatta
    44521 tcaataagct gcggcacact ttcgtgatcc agtcgcgcca gtgtttcaag gatatttaaa
    44581 ccagaggttt cccagaaatt attcaccttc gtcggcttgc catgttgaat ggtcaaccag
    44641 ccatctcgtg ccagacgctg taacacttca cgtaacgtag tacgcgttac gccaattaat
    44701 tctgaaagtt cacgttctgc gggcaaaata gtcccgggag ggaagcggtt attccagata
    44761 ctttcaataa tgtactcttc cgcgaaaccc gccgggcttt gcgccttaat gaccatagtg
    44821 agatttccat aacacagcaa aacaaagttg gactcatcat accagagggc taactacgct
    44881 gatagcagac aaaaagaaga aaaaagggat caaggcttca ttttccgcat ctctttcccc
    44941 ttgactatca agacttgcct cactcctgcc tgaccgttaa gctttgcgtt taaacataaa
    45001 aacatcattg attatttgta aggtaaggga aaccatcatg gagatctcct ggggccgcgc
    45061 gctatggcgc aattttttgg gccagtcccc cgactggtac aaactcgccc tcatcatttt
    45121 cttaatcgta aacccgttaa ttttcctcat cagccctttc gtcgcgggct ggttgctggt
    45181 agcggaattt attttcactc tggcgatggc cctgaaatgc tacccgctgc tccccggtgg
    45241 tctgttggct atcgaagcgg tattcatcgg catgaccagc gcggaacacg tccgtgaaga
    45301 ggtggcggca aatcttgaag tcttgctgtt attgatgttt atggtggcgg gtatctattt
    45361 tatgaaacag ctgttgctgt tcatatttac ccgtttgctg ttaagcattc gctccaaaat
    45421 gctgctgtcg ctctcttttt gcgtggcggc agcgttcctc tccgcgttcc tcgatgcctt
    45481 aaccgtcgtg gcggtggtga tcagcgttgc tgtcggtttt tatggtattt atcaccgcgt
    45541 agcctcttcc cgtaccgaag ataccgacct gcaagacgat agtcatatcg acaagcatta
    45601 caaagtggtt ctggaacagt tccgtggctt tctgcgtagc ctgatgatgc atgccggtgt
    45661 cggcactgca ttaggcggcg tgatgaccat ggtgggcgaa ccacagaacc tgatcatcgc
    45721 taaagcggct ggctggcatt ttggcgattt cttcctgcgc atgtcgccgg tgaccgttcc
    45781 ggttctgatt tgtggcctgt taacctgcct gctggtagag aagctgcgtt ggtttggcta
    45841 cggtgaaacg ctgccggaga aagtccgcga agtgttgcaa cagtttgacg atcaaagccg
    45901 ccaccagcgt acccgtcagg ataaaatccg tctgattgtc caggcgatta ttggcgtctg
    45961 gctggtgact gcgctggcgt tgcatctggc ggaagttggc ttgattggtt tgtcagtcat
    46021 tattctggcg acatcattga ccggtgtcac cgatgaacat gctatcggta aagccttcac
    46081 cgaatctctg ccattcaccg cactgttgac ggtgtttttc tcggtagtcg cggtgattat
    46141 cgaccaacaa ctgttttcgc caattattca gtttgtgttg caggcatcgg aacatgctca
    46201 gctgtcgctg ttctatattt tcaacggtct gctgtcatcc atttcggata acgtcttcgt
    46261 ggggacgatt tatatcaacg aagcgaaagc ggcaatggaa agtggcgcta tcacgttgaa
    46321 gcaatacgag ctgctggcgg tcgccattaa taccggtacc aatctgccct ccgtcgctac
    46381 gccgaacggt caggctgcgt tcctgttcct gctgacctct gcactcgcgc cattgattcg
    46441 cctctcttat ggccgcatgg tgtggatggc cctgccttac accctcgtac tgacactcgt
    46501 cggcttgctc tgcgtcgagt ttacgcttgc ccctgtaacc gaatggttta tgcaaatggg
    46561 ctggatagca acgctttgat aacaacttac cgggcatata aatgcccggt ttgccttttc
    46621 gcccgataat tgtccaattg cttcctattt ttgctgcctc ctggtggcgc agcgaatgaa
    46681 ttggtttaaa ctgcgcactc tatgcatatt gcagggaaat gattatgttg cgatttttga
    46741 accaatgttc acaaggccgg ggcgcgtggc tgttgatggc gtttactgct ctggcactgg
    46801 aactgacggc gctgtggttc cagcatgtga tgttactgaa accttgcgtg ctctgtattt
    46861 atgaacgttg tgcgttattc ggcgttctgg gtgctgcgct gattggcgcg atcgccccga
    46921 aaactccgct gcgttatgta gcgatggtta tctggttgta tagtgcgttc cgcggtgtgc
    46981 agttaactta cgagcacacc atgcttcagc tctatccttc gccgtttgcc acctgtgatt
    47041 ttatggttcg tttcccggaa tggctgccgc tggataaatg ggtgccgcaa gtgtttgtcg
    47101 cctctggcga ttgtgccgag cgtcagtggg attttttagg tctggaaatg ccgcagtggc
    47161 tgctcggtat ttttatcgct tacctgattg tcgcggtgct ggtggtgatt tcccagccgt
    47221 ttaaagcgaa aaaacgtgac ctgttcggtc gctaatccct tcagcgctcc tgcgggagcg
    47281 cttttttcct gccgctatat ttatttgacc cgcagtaaat cagaacttcg cgttgtataa
    47341 cgtggtgaaa gcatggcacg cttcatctgc cattgttgct ggatcccctg cccggcaaaa
    47401 tagagtgttc ctctgccctc tttagcattc agtgtatcca ttaccgtcat caattgctca
    47461 ctcccggggc gcggtgcgtt gtcatcgaat aaattgagct gcgcgactcc ctgactgaag
    47521 aaatccccca gcatcacgcc cgctttttgg tagcgatggc ccgcttgcca gatggcatcc
    47581 agagatcgcg tagcagcgtt aatgatatcc ctgctgtcct gagtgggcgt cagcagtttt
    47641 accgacgcgc tattgccgta ataaggttca ttgagcgcaa atggtgacgt cttaataaat
    47701 gtggagataa accgacaata ttgatgctcg ctgcgaagtt tttccgccgc ccgggcagcg
    47761 taactacaaa tggcctgccg catcgatgga taatccgtga tgcgttcacc aaacgagcgg
    47821 gaacagataa tttcctgctt cgtcggtgca aactcttcca gttgcaaaca gggttcgccg
    47881 cgcagttcac gcaccgttct ttcgagcacg acattaaaat gtttacggat aaaccggata
    47941 tctgtatccg ccaaatcgag aacggttttg atccccatcg cgtccagttt tttgctgatc
    48001 cgccgtccaa tcccccagac gtcatccacg gggagagcag acattaattt acgctggcgt
    48061 tccagatttg ataaatccac caccccaccc gtctgccgct gccatttttt tgccgcatga
    48121 ttggcaagct tagccaacgt tttggtctgg gcgatcccca caccaacagt aagatgggta
    48181 cgttgtagca ccgttgcgcg aatttctctg ccaaaatcag tcagatcgcg acaattacgc
    48241 acacctgtca gatcgcagaa tgcctcatca atactgtaaa tctcgacgcg gggcgatagt
    48301 tcttccagcg tcgacatcac ccgattgctc atgtctgcgt aaagctcata attgctgcta
    48361 aagcaaacca cgccacagcg acgaaacaga tctttttgtt tgaaccaggg atcgcccatt
    48421 ttaacaccca gcgcctttgc ctcagcgttt cgggcgataa cgcaaccgtc attattcgat
    48481 agcacaacca ccggtttacc ccataaatca gggcgaaaca ccgtctcaca gctggcataa
    48541 aacgcgttta catcacagag agcaaacatc agcgcatcgc cttaacgacg tggatcacca
    48601 caccaaagac atccagcgta tcttcgctac tgatggtaat gggcgagtac gcactgttca
    48661 tgggaataag ctgtaccgtc gggcgtagtt gcaatttttt caccgtaaac tcgccgtcaa
    48721 cagcagcgat gacaatatca ccatggctgg cggtaatagc gctatcgaca atcagtaaat
    48781 caccgtcact aattccacca tcaatcatag aatcaccact tgctttgacg aagtaagtcg
    48841 cgctgggatg ctggatcaac agttgattca gatcgatgcg ctgttcaacg taatctgctg
    48901 ccggtgaagg aaagccacac tgaacaagat cgctaaatag cggaaaagtc acaatttcgc
    48961 ggagatccgc aggcttgata aacaacataa taatctgcct gaagttatac tgtttttata
    49021 tacagtagtc tgttcttgcc agcagatcaa tactgattca ggctatcaat atttgtcgct
    49081 gcatagactg ctgatttttc gttctcttat cctgtgctca cgtggccttc tggcgacgac
    49141 gctcatccag cagaaatgaa aaatatcacc cggcaaaaaa atagaataga agcattcgcc
    49201 ataatgacat taaacattgt ttgatattta tcatattaat agaaataaag acattgacgc
    49261 atcccgcccg gctaactatg aattagatga agtaaaattt attaatagtt gtaaaacagg
    49321 agtttcatta caatttatat atttaaagag gcgaatgatt atgactgaaa tcgttgcaga
    49381 taaaacggta gaagtagtta aaaacgcaat cgaaactgca gatggagcat tagatcttta
    49441 taataaatat ctcgatcagg tcatcccctg gcagaccttt gatgaaacca taaaagagtt
    49501 aagtcgcttt aaacaggagt attcacaggc agcctccgtt ttagtcggcg atattaaaac
    49561 cttacttatg gatagccagg ataagtattt tgaagcaacc caaacagtgt atgaatggtg
    49621 tggtgttgcg acgcaattgc tcgcagcgta tatttttcta tttgatgagt acaatgagaa
    49681 gaaagcatcc gcccagaaag acattctcat taaggtactg gatgacggca tcacgaagct
    49741 gaatgaagcg caaaaatccc tgctggtaag ctcacaaagt ttcaacaacg cttccgggaa
    49801 actgctggcg ttagatagcc agttaaccaa tgatttttca gaaaaaagca gcttttccag
    49861 tcacaggtag ataaaatcag gaaggaagcg tatgccggtg ccgcagccgg tgtcgtcgcc
    49921 ggtccatttg gattaatcat ttcctattct attgctgcgg gcgtagttga agggaaactg
    49981 attccagaat tgaagaacaa gttaaaatct gtgcagaatt tctttaccac cctgtctaac
    50041 acggttaaac aagcgaataa agatatcgat gccgccaaat tgaaattaac caccgaaata
    50101 gccgccatcg gggagataaa aacggaaact gaaacaacca gattctatgt tgattatgat
    50161 gatttaatgc tttctttgct aaaagaagcg gcaaaaaaaa tgattaacac ctgtaatgag
    50221 tatcagaaaa gacacggtaa aaagacactc tttgaggtac ctgaagtctg ataagcgatt
    50281 attctctcca tgtactcaag gtataaggtt tatcacattt aaaaacacag acgtttttta
    50341 aagattgaga gttacctgtt gataataagg tatcgggtaa ctctcattat gacagagcgc
    50401 tttcaaaaac agggctacga aatggcgagc atccagacgt atattaagaa agcccccatc
    50461 aacatctatt aaatttatca ctgcgcccag tcaggtttat ttaagatatg atcctgccag
    50521 tcaatcactt ctgattcttt cactgcgata tgacgcacag agatacgttc accatgcatt
    50581 gccgctttcg aaccagtaag tagcggatgc cacgcaggta aatctttacc ttctgccagc
    50641 aaacgataag cgcaggtcat tggcagccat tcgaatgttg gcagattatc acgggttaat
    50701 ttaatgcagt cgggttcaaa ctcaaaacga cgttcgtagt tccgacattg acaggtttta
    50761 atattgagct ggcgacaggc gacgttagtg aagtagattt cgtcggtgtc ttcatccatc
    50821 agtttatgca ggcaacactg accgcaacca tcacacaacg actcccattc cgcatcgctc
    50881 atttcgtcca gggttttact ttgccagaaa ggtacatcgc tcatcaggtg ttccgccatt
    50941 acgttaaaac gcaccttata accagtctgg cacagcgatg caagttttgc cgccgctttc
    51001 aggcggcaaa aagtattaca aaacgcgagt tgtcaaagaa tgcccatcga aagtgactgt
    51061 cagctcatca ccgctttgca acgggccgac gccatcaggc gtgcctgtca acacaacgtc
    51121 accggccttg agggtaaaaa acttgctcat ataagcgatc agcggaatga ttttatggat
    51181 catgtccgca gtcgtacctt gctggcgttg ttcgccgttt acgctcaggc ccagcgttgt
    51241 attttgcgga tcgccggtaa attccgccgc gggaataaac ccggaaagcg gacaagagtt
    51301 atcaaacgct ttagcctttt cccacggctg cccggctttc ttcatttttc cctgaacatc
    51361 acgcaatgtc agatcaagcg ccacgccgta accggcaatg gctttgcgga catgctcttc
    51421 cgtagcctga cgcagcgtcg cgccaatcaa caccgccagt tcgacttcat gatgaactga
    51481 accgaaatcg gatgggatcg ccaatggctg ccgcagatcg cacagtgccg tttctggttt
    51541 aataaacagc actggctctt cgggcactgc gctgcccatc tctttaatat gtttggcata
    51601 gttactgcca acacagacta ctttactcac cggataatcc agcagcgcac cttgccagtt
    51661 gtgatgttga tacataacgt tcccctcacc ttatcgtgga tatgaaaacc cgcgccgaca
    51721 aagataatgc caccgccgga tatcggttat ttgttagtgt cgtctgtttt ctgccccatg
    51781 acggaaagat gttgcttcag caaatcttcg ggtggtggcg gtaactgcaa atagtaacct
    51841 tgctcggtta atgcctgttt aaccttttca atatcggcat tgaccagttt cttccgccca
    51901 tccagcggca gaatcatcgc taactgaggc tgaccaaaac ctttcatcag ttcctcagga
    51961 acacgcgaaa aatcgtcttt tttttcgaca tataaatagg tctggtcacg cttgctgctt
    52021 cgataaatca cacaaaacat acttttactc tttaagatgc caggttttgg cataagctaa
    52081 catcaatgaa acccgcgcca caagccagct agctgtagat ttctacggtt tgttgagtcc
    52141 atgccccctt tataagtcat ccccccggca aaaatgccgc gacgggattt taccgaacgc
    52201 aggaaagcca ctggaattct cccgtaaggg attgttatat tataacagtt catcgtactc
    52261 attctgaaca ggagactacc aatgaaaatc aagagcatca gtaaggctgt attgctgctt
    52321 gcactgctaa cctccacgag ctttgctgcg ggtaagaatg tcaatgttga gttcagaaaa
    52381 ggccatagca gcgctcagta ctccggcgaa ataaagggat acgattacga tacatatacc
    52441 ttctatgcca aaaaaggtca gaaagtgcat gtgagtatct ctaatgaagg cgctgatact
    52501 tacctcttcg ggccaggaat cgatgattct gttgatttat ccagatattc cccagaactc
    52561 gatagtcacg gacaatactc actccctgct tctggcaaat atgagctgcg agtgctgcaa
    52621 acccgaaacg acgcccgtaa aaacaaaact aaaaaatata acgtcgatat tcaaattaag
    52681 taaatgtcaa ccgacgggga ctctccccgt cataaacgtt aacgccccac tcctctctgt
    52741 cgctcaagag gttaatgacg aatagcagac aatgccgaat agaacataat gtaaacaagg
    52801 catcactcac gacacataaa taacatcttt gtggtggttt gattgatttt tccactccgc
    52861 ttaccatcta caccaaaata acgatcatca cgacataatt tttctttaac atcgcaaaat
    52921 acaccgttag cgaatgtaaa ttcgctggga tcgtagcctt gtaatgattg taagttttca
    52981 gcggcttttt cgccgagata tttccttgtt aactctgggg aaatgccatc tgagttcaca
    53041 caaatatacg ggtcgcaaac aacgccgcac gccggagatt gaagtccgcc agcgtcattt
    53101 ccagacgcca ttgatgagaa catcatcccc acacctgaca atccgatata aaaaatacgc
    53161 atcatattca tagcacatcc tgataatcca accgtacaac acttatgctc atttcatcat
    53221 caatccgtaa tcaaaaccgc tgacctggac gagttacgat gacctgacag aagtatcaga
    53281 cgtcttctgc ggtttctatg atagagcaat tctgtggcgg atattaagat aacgggtatc
    53341 tgttctttaa tgatgccgca aaaaatggaa agttaagaaa gcctggcgtg gtgaaaaaat
    53401 acacttgcct caatactgga tatttaaaca ggtctggaga tgctgtcacg attagggtgc
    53461 ggaaaaagga agcgtaaata agaagaatca aaagtacgac ggcaatgggt tgattgacaa
    53521 gggtattttt taagctatga atcagcgcca tttatcacag aatagacttt tactctgaat
    53581 aaatgggagg gtgacttgcc tcaatataat ccagactata acatgcctta tagtcttcgg
    53641 aacatcatcg cgcgctggcg atgattaata gctaattgag taaggccagg atgtcaaaca
    53701 cgccaatcga gcttaaaggc agtagcttca ctttatctgt ggttcatctg catgaggcag
    53761 aacctaaggt tatccatcag gcgctggaag acaaaatcgc tcaggccccc gcatttttaa
    53821 aacatgcccc cgttgtactc aacgtcagtg cactggaaga cccggtaaac tggtcagcga
    53881 tgcataaggc ggtttcggca accggtttgc gggttattgg cgtaagtggc tgcaaagatg
    53941 cgcaacttaa agccgaaatt gaaaaaatgg ggctgcctat cctgacggaa ggaaaggaaa
    54001 aagcgccgcg tccagctccc gcaccacagg ctccagcgca aaatacaacg ccggtcacaa
    54061 aaacgcgttt aatagatacc ccggtgcgtt ccggtcagcg tatttatgct ccacaatgtg
    54121 atctgattgt tacaagccac gttagcgctg gggccgaatt gattgccgat gggaacattc
    54181 atgtctatgg catgatgcgc ggtcgtgcgc ttgcaggggc aagtggtgac cgggaaacgc
    54241 aaatattttg tacgaacctg atggcggaac tggtgtccat cgcaggtgaa tactggctga
    54301 gtgatcaaat cccagcagaa ttttatggca aagcggcgcg actgcagtta gtcgaaaacg
    54361 ctttgaccgt tcaaccgtta aattgatccc tttttaacaa ggaatttcta tggcacgcat
    54421 tattgttgtt acttcgggca aagggggtgt tggtaagaca acctccagcg cggccatcgc
    54481 cactggtttg gcccagaagg gaaagaaaac tgtcgtgata gattttgata tcggcctgcg
    54541 taatctcgac ctgattatgg gttgtgaacg ccgggtcgtt tacgatttcg tcaacgtcat
    54601 tcagggcgat gcaacgctaa atcaggcgtt aattaaagat aagcgtactg aaaatctcta
    54661 tattcttccg gcatcgcaaa cacgcgataa agatgccctc acccgtgaag gggtcgccaa
    54721 agttcttgat gatctgaaag cgatggattt tgaatttatc gtttgtgact ccccggcagg
    54781 gattgaaacc ggtgcgttaa tggcactcta ttttgcagac gaagccatta ttaccaccaa
    54841 cccggaagtc tcctcagtac gcgactctga ccgtatttta ggcattctgg cgtcgaaatc
    54901 acgccgcgca gaaaatggcg aagagcctat taaagagcac ctgctgttaa cgcgctataa
    54961 cccaggccgc gtaagcagag gtgacatgct gagcatggaa gatgtgctgg agatcctgcg
    55021 catcaaactc gtcggcgtga tcccagagga tcaatcagta ttgcgcgcct ctaaccaggg
    55081 tgaaccggtc attctcgaca ttaacgccga tgcgggtaaa gcctacgcag ataccgtaga
    55141 acgtctgttg ggagaagaac gtcctttccg cttcattgaa gaagagaaga aaggcttcct
    55201 caaacgcttg ttcggaggat aagttatggc attactcgat ttctttctct cgcggaagaa
    55261 aaacacagcc aacattgcaa aagaacggct gcagattatt gttgctgaac gccgtcgcag
    55321 cgatgcagaa ccgcattatc tgccgcagtt gcgtaaagat attcttgagg tcatttgtaa
    55381 atacgtacaa attgatcctg agatggtaac cgtacagctt gagcaaaaag atggcgatat
    55441 ttctattctt gagctgaacg tgaccttacc ggaagcagaa gagctgaaat aagcccgctg
    55501 taaaaacgca tttatcttca aggcagagtc atttctgcct tgagtttttc atccctctca
    55561 tccacgttgt ggtaaagcgg cgagtattct tgctgatact cctcattgct atttccacct
    55621 ccccctttac accttaaggc tgttcccacg cacagaacaa aatgtttatg cctggttgag
    55681 taaataaccc tgttgttagt gtggatatac tgggagatga taaccccgca atttcattat
    55741 tcattgttct cccataggat gaggtttccc gccgtaactg gcgggctttt tttgccccag
    55801 aattttctct ttcaacatcc tgtaaacgaa aactgcgccg aagcgcagtt taatcgttga
    55861 tggtcagaac gaccagttca caccagccac cgcgttccac ggggactcca caccggcacc
    55921 atggctatac cccaccccga aatgcccgct taacgtactg ctgaatgagg ctttaatacc
    55981 tgcctggtat attccacgtc tgcccgacaa atcattgaca aaattaccgt cactattcac
    56041 tttcacccga ttatcatcga caaattcttt gcgcacagcc gccttcaggc agggttcaat
    56101 ttccataccg ttccccagac gcatgttgta actcagcgtt gcacccagtt cacgatatat
    56161 actgcgggta tcgactgatt tcgattccat gccattggat aaatgatagt agcgtctaag
    56221 tgacctaagc tctgctgcaa catcagcccg caggcaccag accaggggat tcatcctgaa
    56281 gagacagcgc aggtgtattg tgttcaccgc tcaccaaaga catcatgatg aaatgatgat
    56341 atcccgcata agaaagaggc attttttaca cgcagtgcgc tgaagtgtag ttggataaaa
    56401 aatcactctt ttcagataat actgtcgtac gcttatcttt cgacaatgag gccgtcggca
    56461 aaataaaatg actaatgtaa tcgttgctga tgaatatctt ctgctcacat aaaaatcaca
    56521 caataatatt gataaatcag attatcttac ctttacaaca ttgcgttaac agtcaatgac
    56581 acttcttgaa taaaaatatt gtcaatcaat gagttattcc tcatttaaga ttattctcat
    56641 tcacgtaaaa aacataattc ctatcatcac agccacgctc attttgaatt atctgaataa
    56701 tagcgagcgt tctttagtta agcagatctt aattgaagac gaaattattg tgtgtgcaac
    56761 ttacttaata ccggacatct aagttgaaga aaaaaatact tgccttcggg cttatctcag
    56821 cattgttctg ctccacgcca gcgatggcgg atatgaatcg caccacgaaa ggtgcattgc
    56881 taggcgcagg cgttggttta cttactggca atggtgttaa tggcgtactg aaaggtgcag
    56941 ctgttggcgc tggtgttggt gcagtaacag aaaaaggccg cgacggtaaa aatgcgcgta
    57001 aaggtgcaaa agtaggtgcc gccgttggtg ctgtcactgg cgttctgacc ggcaatggcc
    57061 tcgaaggggc gattaaaggt gccgtgattg gcggtacggg tggtgcaatt ctgggtaaaa
    57121 tgaaataagg aatgatgatg aaaaaatttg ctttactggc gggcctgttt gttttcgcac
    57181 caatgacctg ggcgcaggac tacaatatta aaaacggttt gccgtcagaa acctatatta
    57241 cttgcgcaga agccaacgaa atggcaaaaa ctgacagcgc acaggttgca gaaattgttg
    57301 cggtaatggg taatgccagc gttgccagcc gtgatttaaa aattgagcaa tctccggaac
    57361 tgagcgccaa agtggtagaa aaactgaatc aggtttgcgc taaagacccg caaatgctgt
    57421 tgatcacggc aattgatgac accatgcgtg ctatcggcaa aaaataatat ttcatatgta
    57481 ccgcgtcgat gaatacgacg cggtttttta gctaacggcg ggatcagaac ttatatttca
    57541 ggcccaccat cactgcggtg tcattgtagc cattatcacc cagctgcacg cccacattcc
    57601 cccagacgct ggccgctgga ttcaggttgc catttacgcc aagtttaatt tcccccaaat
    57661 tactcgcccc atcttgttcg actttcacac cgttcattga gacagcaaaa tctttactgt
    57721 tatggatcca gttcatttca acaaaaggtt caaagtcgtc accgtgcgga ccgctgtttt
    57781 tctcctgagt acgaataaag gtgcggaaac caagtcgggt ctggatgttg ttatttgcgt
    57841 cattctcaat acgtgagcca ttggcttcgg tgtgcgcatt atgatcgacg cccatccaga
    57901 tagcctgtgc ctgtggctgg aatatccacg tgtatttcgc cgcttccaga ccaaaggatt
    57961 tattcagggc aaagccgtaa cccgcttcca gcgagacggt tgcgcctttc gcactatagg
    58021 attcataggc taattcatcc cctttcaccg agtttttaaa ccagctatat tgcgcccagg
    58081 cgtcaatgta tgcgcctttt ttactgatgt catcggcaaa ccaggtggca tacagccctg
    58141 cgcaatagcc tctgacactc cctttcgaac gataatccga cacgctggaa tgagttgagt
    58201 tgtagtcgcg ggcataacca gccatcactc ccaaacgcca actgtcactg tcggtaaaac
    58261 caccggttaa cagatcggcc cctaactgcg agacgtagcg atgcgaggtc gttctcaact
    58321 gtccgttgct gtcacgccag gcattatgcc cgccaatttg acgtagccaa agtcggcttg
    58381 aacgctcctg ttcagttaca ggatcgatgt agcgcatttc acccgcacgg tcgttcagac
    58441 gcataacaaa aagagtgtta gcggctgcca ggttggcgac gtaacttcca gcttcgaggc
    58501 gcaggacggg ggtaggcgca acagtaggcg ctgaacttcc tccggagttt gtttcaccgc
    58561 cgctcgttgc ctggaagtta gtcagatacc agtttttatt ggtcgcttcg gtattacctc
    58621 gggttaatga atattcgtag gcaccggcga aaatcctcga atccttaata aattccccat
    58681 ttgattcccc ctcaacgctg ataatctcta tcccatttaa cgtgtaagct ccgctgcctc
    58741 ctgcattggt aactttaact cgggttgttc cagaggtatt tccattaata acgactttat
    58801 ccgtagctga attatcatca cccaattcag ttctgagatg caatacgcca ttattaccaa
    58861 cgtaattttc agttattgtt aatcttgttg gttcgacatc ccgtccatct gcccgggaaa
    58921 tatagaccgt actattatca accgtgaggt tgctcaccgt tgagtcagct ttaatatccc
    58981 aggtactatt atctgaaagc gacagatagg tatgagtgtt aacatctgtt gaaatattag
    59041 ctgaacccgt tatttttgaa ttagttgcat cgacggtcaa atgaccttta atattattga
    59101 taaaaacatc tgcagccgat gatacctcac tatccgtaat ggtcacgtta ctcgtctcat
    59161 ttttatggta gaggatttca tttgctgacc ctagtgagct accagataaa atatctaccg
    59221 ttgcttcctg accatttatg gaagactcca gcacaattcc agacccctga tgcgcgactg
    59281 cagatgtatt tttcagcgtc accgtgccgc cattataaat tttagcagtc gcggcgtcag
    59341 gacccgttgt ataaatttct ccctggttaa atgaaactgt cccctggcgg gcagcgatgg
    59401 catgagcatt gtccccatgc gtggtgatat caacatcggt taatgtaacg ctggcattgg
    59461 tataatcaag cgtgtcacca gccttgacga cagtgccatt agcgtacgcg gcataacttt
    59521 ctgttccgtg ggttgtgatc tccccttttg ccatatccag ttttgctatg gcattatttt
    59581 cattacttcc tgtatcggta gtactttctt catcaagaga agatacacac gcacgcgcgc
    59641 ttccgggaga tgcacacgca agaacgccga ctgcatgatt acccgcagta acaataaatg
    59701 tatcatctcc gtcagaaaaa gagggggtaa ccgaaccgtc agagttgact cttattatgg
    59761 tgtcagtatt caggtttcca ctcgtttgac ttagcaaacc aattgagtaa tcccccgtta
    59821 tgacaatcgt gtttttaccc tgcaaaataa cagcaccgcc aaattcactt acgattgcgc
    59881 tagctgttaa tgctgtgctt agaggactat tgtcagaagg atataaaccg cccgttgtta
    59941 caatgacgcc attgttaatg gttaacttgc taccctctcc tgcaacggat acccgtaact
    60001 tccattaccc gttgttataa ctagcgcatt atcaatatct tttacttttc catcttttat
    60061 tgccacagca tgtttatatt catcgtgctg aataaactgt tcagcatatg cattacccac
    60121 cgccagagca ataagtccca aactaaaccc ggagagtttt ttcagtttca tagattaacc
    60181 atccattcat tggattaaca aacattcctg gtcaaataac ctatgcgttc agattaccaa
    60241 aacggagggt aaagacaaaa acaaaagcac aaaaaattta aatagataaa cagcg
//
LOCUS       DAZGSP010000016        58512 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZGSP010000016 DAZGSP010000000
VERSION     DAZGSP010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.016"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            319..990
                     /locus_tag="ACHK74_001141"
     CDS             319..990
                     /locus_tag="ACHK74_001141"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFP0219999.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            1000..2196
                     /gene="yaiP"
                     /locus_tag="ACHK74_001142"
     CDS             1000..2196
                     /gene="yaiP"
                     /locus_tag="ACHK74_001142"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP0220000.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2174..2755
                     /locus_tag="ACHK74_001143"
     CDS             2174..2755
                     /locus_tag="ACHK74_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP0220001.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2757..3530
                     /gene="yaiO"
                     /locus_tag="ACHK74_001144"
     CDS             2757..3530
                     /gene="yaiO"
                     /locus_tag="ACHK74_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFP0220002.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETENFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3714..3989
                     /gene="frmR"
                     /locus_tag="ACHK74_001145"
     CDS             3714..3989
                     /gene="frmR"
                     /locus_tag="ACHK74_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFP0220003.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            4024..5133
                     /gene="frmA"
                     /locus_tag="ACHK74_001146"
     CDS             4024..5133
                     /gene="frmA"
                     /locus_tag="ACHK74_001146"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFP0220004.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5226..6059
                     /gene="fghA"
                     /locus_tag="ACHK74_001147"
     CDS             5226..6059
                     /gene="fghA"
                     /locus_tag="ACHK74_001147"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP0220005.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6284..6823)
                     /gene="yaiL"
                     /locus_tag="ACHK74_001148"
     CDS             complement(6284..6823)
                     /gene="yaiL"
                     /locus_tag="ACHK74_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFP0220006.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6925..8136)
                     /gene="mhpT"
                     /locus_tag="ACHK74_001149"
     CDS             complement(6925..8136)
                     /gene="mhpT"
                     /locus_tag="ACHK74_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFP0220007.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8312..9325)
                     /gene="mhpE"
                     /locus_tag="ACHK74_001150"
     CDS             complement(8312..9325)
                     /gene="mhpE"
                     /locus_tag="ACHK74_001150"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFP0220008.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9322..10272)
                     /gene="mhpF"
                     /locus_tag="ACHK74_001151"
     CDS             complement(9322..10272)
                     /gene="mhpF"
                     /locus_tag="ACHK74_001151"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFP0220009.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10269..11078)
                     /gene="mhpD"
                     /locus_tag="ACHK74_001152"
     CDS             complement(10269..11078)
                     /gene="mhpD"
                     /locus_tag="ACHK74_001152"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFP0220010.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11088..11954)
                     /gene="mhpC"
                     /locus_tag="ACHK74_001153"
     CDS             complement(11088..11954)
                     /gene="mhpC"
                     /locus_tag="ACHK74_001153"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFP0220011.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11972..12916)
                     /gene="mhpB"
                     /locus_tag="ACHK74_001154"
     CDS             complement(11972..12916)
                     /gene="mhpB"
                     /locus_tag="ACHK74_001154"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFP0220012.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12918..14582)
                     /gene="mhpA"
                     /locus_tag="ACHK74_001155"
     CDS             complement(12918..14582)
                     /gene="mhpA"
                     /locus_tag="ACHK74_001155"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFP0220013.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14773..15606
                     /gene="mhpR"
                     /locus_tag="ACHK74_001156"
     CDS             14773..15606
                     /gene="mhpR"
                     /locus_tag="ACHK74_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFP0220014.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQD
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15683..16765
                     /gene="lacI"
                     /locus_tag="ACHK74_001157"
     CDS             15683..16765
                     /gene="lacI"
                     /locus_tag="ACHK74_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFP0220015.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLLINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16888..19962
                     /gene="lacZ"
                     /locus_tag="ACHK74_001158"
     CDS             16888..19962
                     /gene="lacZ"
                     /locus_tag="ACHK74_001158"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFP0220016.1"
                     /translation="MSMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHIIPQ
                     LTTSETDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            20014..21267
                     /gene="lacY"
                     /locus_tag="ACHK74_001159"
     CDS             20014..21267
                     /gene="lacY"
                     /locus_tag="ACHK74_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFP0220017.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(21346..22527)
                     /gene="cynX"
                     /locus_tag="ACHK74_001160"
     CDS             complement(21346..22527)
                     /gene="cynX"
                     /locus_tag="ACHK74_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFP0220018.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPMQLPVLWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGLLRSISGNYLMDWAFHTLCVVGLMIITLRFAPARFPQLWVKE
                     V"
     gene            complement(22533..23003)
                     /gene="cynS"
                     /locus_tag="ACHK74_001161"
     CDS             complement(22533..23003)
                     /gene="cynS"
                     /locus_tag="ACHK74_001161"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFP0220019.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGVKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(23034..23693)
                     /gene="cynT"
                     /locus_tag="ACHK74_001162"
     CDS             complement(23034..23693)
                     /gene="cynT"
                     /locus_tag="ACHK74_001162"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFP0220020.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            23803..24702
                     /gene="cynR"
                     /locus_tag="ACHK74_001163"
     CDS             23803..24702
                     /gene="cynR"
                     /locus_tag="ACHK74_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFP0220021.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAKHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(24742..26025)
                     /gene="codA"
                     /locus_tag="ACHK74_001164"
     CDS             complement(24742..26025)
                     /gene="codA"
                     /locus_tag="ACHK74_001164"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFP0220022.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(26015..27274)
                     /gene="codB"
                     /locus_tag="ACHK74_001165"
     CDS             complement(26015..27274)
                     /gene="codB"
                     /locus_tag="ACHK74_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFP0220023.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(27511..29397)
                     /gene="prpE"
                     /locus_tag="ACHK74_001166"
     CDS             complement(27511..29397)
                     /gene="prpE"
                     /locus_tag="ACHK74_001166"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFP0220024.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRYQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVSVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDGRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(29437..30888)
                     /gene="prpD"
                     /locus_tag="ACHK74_001167"
     CDS             complement(29437..30888)
                     /gene="prpD"
                     /locus_tag="ACHK74_001167"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFP0220025.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(30922..32091)
                     /gene="prpC"
                     /locus_tag="ACHK74_001168"
     CDS             complement(30922..32091)
                     /gene="prpC"
                     /locus_tag="ACHK74_001168"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFP0220026.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLNLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRPFVALDKRQ"
     gene            complement(32136..33026)
                     /gene="prpB"
                     /locus_tag="ACHK74_001169"
     CDS             complement(32136..33026)
                     /gene="prpB"
                     /locus_tag="ACHK74_001169"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFP0220027.1"
                     /translation="MSLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            33265..34851
                     /gene="prpR"
                     /locus_tag="ACHK74_001170"
     CDS             33265..34851
                     /gene="prpR"
                     /locus_tag="ACHK74_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFP0220028.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAVIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAVTVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFTQLLLPELARESAKIPAPRLLTPQQALEKFKGDK
                     TAAANYLGISRTTFWRRLKN"
     gene            complement(34952..35227)
                     /gene="yahO"
                     /locus_tag="ACHK74_001171"
     CDS             complement(34952..35227)
                     /gene="yahO"
                     /locus_tag="ACHK74_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFP0220029.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            35374..36045
                     /gene="yahN"
                     /locus_tag="ACHK74_001172"
     CDS             35374..36045
                     /gene="yahN"
                     /locus_tag="ACHK74_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFP0220030.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(36062..36307)
                     /gene="yahM"
                     /locus_tag="ACHK74_001173"
     CDS             complement(36062..36307)
                     /gene="yahM"
                     /locus_tag="ACHK74_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFP0220031.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRGRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(36719..37534)
                     /gene="yahL"
                     /locus_tag="ACHK74_001174"
     CDS             complement(36719..37534)
                     /gene="yahL"
                     /locus_tag="ACHK74_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFP0220032.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNQWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(37777..38826)
                     /gene="yahK"
                     /locus_tag="ACHK74_001175"
     CDS             complement(37777..38826)
                     /gene="yahK"
                     /locus_tag="ACHK74_001175"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFP0220033.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(38913..39869)
                     /locus_tag="ACHK74_001176"
     CDS             complement(38913..39869)
                     /locus_tag="ACHK74_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0220034.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            complement(39866..40837)
                     /locus_tag="ACHK74_001177"
     CDS             complement(39866..40837)
                     /locus_tag="ACHK74_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0220035.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(40830..42314)
                     /locus_tag="ACHK74_001178"
     CDS             complement(40830..42314)
                     /locus_tag="ACHK74_001178"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP0220036.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(42363..43346)
                     /locus_tag="ACHK74_001179"
     CDS             complement(42363..43346)
                     /locus_tag="ACHK74_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0220037.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(43601..44011)
                     /locus_tag="ACHK74_001180"
     CDS             complement(43601..44011)
                     /locus_tag="ACHK74_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP0220038.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(44310..45692)
                     /gene="yahJ"
                     /locus_tag="ACHK74_001181"
     CDS             complement(44310..45692)
                     /gene="yahJ"
                     /locus_tag="ACHK74_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP0220039.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(45702..46652)
                     /gene="yahI"
                     /locus_tag="ACHK74_001182"
     CDS             complement(45702..46652)
                     /gene="yahI"
                     /locus_tag="ACHK74_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFP0220040.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(46795..48213)
                     /gene="yahG"
                     /locus_tag="ACHK74_001183"
     CDS             complement(46795..48213)
                     /gene="yahG"
                     /locus_tag="ACHK74_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP0220041.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEATELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(48213..49760)
                     /gene="fdrA"
                     /locus_tag="ACHK74_001184"
     CDS             complement(48213..49760)
                     /gene="fdrA"
                     /locus_tag="ACHK74_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP0220042.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKAGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(49750..50613)
                     /gene="yahE"
                     /locus_tag="ACHK74_001185"
     CDS             complement(49750..50613)
                     /gene="yahE"
                     /locus_tag="ACHK74_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP0220043.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(50653..51258)
                     /gene="yahD"
                     /locus_tag="ACHK74_001186"
     CDS             complement(50653..51258)
                     /gene="yahD"
                     /locus_tag="ACHK74_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFP0220044.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            51516..52013
                     /gene="yahC"
                     /locus_tag="ACHK74_001187"
     CDS             51516..52013
                     /gene="yahC"
                     /locus_tag="ACHK74_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFP0220045.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGISGFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFACGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            52105..53037
                     /gene="yahB"
                     /locus_tag="ACHK74_001188"
     CDS             52105..53037
                     /gene="yahB"
                     /locus_tag="ACHK74_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0220046.1"
                     /translation="MNSVFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(53079..54167)
                     /gene="pdeL"
                     /locus_tag="ACHK74_001189"
     CDS             complement(53079..54167)
                     /gene="pdeL"
                     /locus_tag="ACHK74_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0220047.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(54309..58292)
                     /gene="ehaA"
                     /locus_tag="ACHK74_001190"
     CDS             complement(54309..58292)
                     /gene="ehaA"
                     /locus_tag="ACHK74_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFP0220048.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSQVIVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVSSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT        13950 a        15847 c        14573 g        14142 t
ORIGIN      
        1 gcatattaca ttcacataat gtatataaac aatacttgtt cacagaacat attttttaaa
       61 tgcaattaat tgacaaacta aatcacatct aaatacaatg tataaaacaa aataactata
      121 cataccaaac atacaaacac ttaacatatg aattgcataa aaacattacc cagtcgaacc
      181 tccgggtctg aaagcccttg cttttcagtt attagcaaca tttaacattt ataaattaac
      241 atctggctgc gccagagatt acgtctctga gtgcatcgtt gtgcttgtag tataataaat
      301 aaaatggagt gtcattaagt ggataaggtt ttagattcag ccctcctttc ttcggcaaat
      361 aaaagaaagg gtatccttgc tattggcgca catcctgacg atatagaatt aggctgtggc
      421 gcatcgcttg ctcgtcttgc gcaaaaagga atttatatcg cagcagtggt gatgactacc
      481 ggcaactctg gtacagatgg aataatcgat cgccatgaag aatcgcgcaa cgccctaaag
      541 atattagggt gccaccaaac tattcatctt aattttgctg acacccgcgc tcatttacag
      601 ctcaatgata tgatttccgc cctggaagac atcattaaaa atcaaattcc ttctgatgtt
      661 gaaatcatgc gggtatatac catgcatgat gccgaccgcc atcaggatca tctcgctgtt
      721 tatcaagctt caatggttgc ctgccgcact attccacaaa ttctcggcta cgaaacccca
      781 agtacctggc tttcatttat gcctcaggtt tttgaatccg ttaaagaaga atatttcaca
      841 gtcaaacttg ctgcattaaa aaaacataaa agccaggaac gacgtgatta tatgcgtcat
      901 gatcgcctgc gtgcagttgc acaatttcgc gggcaacagg tcaatagcga tctgggtgaa
      961 ggctttgtaa ttcataaaat gattctttga gcaaattgca tgaaaacctg gatatttatc
     1021 tgtatgtcca tagcaatgtt gctatggttt ttaagtacgc taagacgtaa acccagtcaa
     1081 aagaaaggct gtattgacgc cattatacct gcgtataacg aaggcccgtg tctggcgcag
     1141 tcactggata atctactgcg taacccttat ttttgccggg taatttgcgt taacgacggc
     1201 tccacggaca ataccgaagc ggtcatggcg gaagtcaaac gcaaatgggg cgaccgcttt
     1261 gttgccgtca cgcaaaaaaa taccggtaaa ggtggtgcgc tgatgaatgg cctcaattat
     1321 gccacctgcg accaggtttt tttaagtgat gccgacacct atgttccgcc cgatcaagac
     1381 ggaatgggct atatgctggc agaaattgag cgcggtgctg atgccgtagg cggcattccc
     1441 tctactgcgt tgaaaggcgc ggggctgtta ccgcacatcc gcgcgaccgt aaagttgccg
     1501 atgattgtta tgaagcgcac gctacagcag ctcctgggcg gcgcaccgtt tattatcagc
     1561 ggtgcctgcg ggatgttccg tactgatgta ttgcgtaagt tcggtttctc ggatcgtact
     1621 aaagtcgaag accttgatct cacctggaca ttagtggcaa acggctaccg tattcggcag
     1681 gcgaatcgct gcatcgtata cccacaggaa tgcaacagtc cgcgtgagga gtggcgtcgc
     1741 tggcggcgtt ggattgtggg atacgcggtc tgtatgcgcc tgcataaaag acttttattt
     1801 agccgcttcg gtatcttcag tatatttcct atgctgttgg ttgtgattta tggcgttggg
     1861 atttatctca ctacctggtt taatgaattc atcaccaccg ggccgcatgg tgtggtgttg
     1921 gcaatgtttc cgcttatctg gatcggcgta gtttgtgtta ttggtgcttt tagcgcctgg
     1981 tttcatcgtt gctggttgtt ggtgccttta gcgccgcttt ccgttgtgta tgtattatta
     2041 gcttatgcca tctggattat ttatggactt attgcctttt ttactggacg cgaacctcag
     2101 cgcgacaaac ccacccgcta ttccgcactg gtggaagcgt caaccgctta ttcccaacct
     2161 tctgtcacag gaactgaaaa actatctgaa gcttaatgtt aaagagaaaa atattcagat
     2221 tgcagaccag gtaattattg atgaaactgc aggtgaagtt gttatcggcg cgaatacccg
     2281 tatttgtcat ggtgccgtta ttcagggtcc ggtagtgatt ggcgcaaact gcctgatagg
     2341 taattatgcg tttattcgtc ctggcacaat aatcagcaat ggtgtaaaaa ttggttttgc
     2401 caccgaaatt aaaaatgcgg ttattgaagc ggaagcaacg attggtccgc aatgttttat
     2461 tgccgactcg gtagttgcaa accaggcata tttgggcgca caagtacgta ccagtaatca
     2521 tcgtctggat gaacagcctg tgtctgttcg aactccagag ggaattatcg ctaccggatg
     2581 cgataaatta ggttgttata tcgggcagcg ttcacgcctt ggcgtacaag ttataatttt
     2641 gcctgggcga attatttccc cgaacacgca acttggcccg cgcgtgattg tagaacgtaa
     2701 tttacctgcc ggaacttatt cactccgaca agaacttatc cgtacaggag attaaaatga
     2761 taaaacggac gttattagcg gcggccattt ttagcgcatt gcccgcttat gccgggttaa
     2821 cttccattac cgcgggctac gattttaccg attattctgg cgatcatggc aaccgtaatt
     2881 tagcgtatgc tgaactggtg gcgaaagttg aaaacgcaac gctgcttttt aatctttccc
     2941 aggggcgtcg tgattatgaa actgaaaatt tcaatgccac tcgcggtcag ggtgcggtct
     3001 ggtataaatg gaataactgg ctgacaaccc gaacgggtat tgcctttgcg gataatacgc
     3061 cggtctttgc ccgccaggat tttcgtcagg atattaacct ggccctgttg ccaaaaacgc
     3121 ttttcacgac cggttatcgc tacactaaat attacgatga tgtcgaagtc gatgcctggc
     3181 aaggcggcgt atcactctat actggcccgg tgatcaccag ctaccgctat acccattatg
     3241 actccagcga tgcaggtggt agttatagca atatgatttc cgtgcgtctg aatgacccgc
     3301 gcggcactgg ttatacgcaa ctatggctaa gccgcggaac aggcgcttac acctatgact
     3361 ggacgccaga aacacgctac ggcagcatga agagcgtcag tttgcaacgt attcaaccgc
     3421 tgactgagca acttaatctc gggctgacgg caggtaaagt gtggtacgac accccaaccg
     3481 atgattataa cggtctgcaa cttgcagccc atctgacctg gaaattctga ttccttctgc
     3541 cgcccgctat ccggggcggc cttccctgcc gattagcccc ccctttcctc tttgttttcc
     3601 gaccacattc accggataaa ttttattctc cagtgttata tactataggg gggtatgcat
     3661 tgacatatag cataccccct atagtatatt gcgtgcagat gatgaggtgc gaaatgccca
     3721 gtactccgga agagaagaaa aaggtcctta ctcgagttcg tcgtattcga gggcagattg
     3781 atgctctgga acggtcgctg gagggtgatg ccgaatgccg tgccatactc caacagatcg
     3841 ctgccgttcg gggcgcggct aatgggctga tggcagaagt gcttgaaagc catatccggg
     3901 aaacgtttga ccgaaatgac tgctacagcc gcgaagtcag ccaatccgtt gacgacacta
     3961 ttgaactggt tcgagcctat cttaaatagc tgaatctatt accatattga ggaagagcga
     4021 gagatgaaat cacgtgctgc cgttgcattt gctcccggta aaccgctgga aatcgttgaa
     4081 attgacgttg caccaccgaa aaaaggtgaa gtgctgatta aagtcaccca taccggcgtt
     4141 tgccataccg acgcatttac cctctccggg gatgacccgg aaggtgtatt cccggtggtt
     4201 ctcggtcacg aaggggccgg cgttgtggtt gaagtcggtg aaggcgtaac cagcgtcaaa
     4261 cctggcgacc atgtgatccc gctttacacc gcggagtgcg gcgagtgtga gttctgtcgt
     4321 tctggcaaaa ctaacctctg tgttgcggtt cgcgaaactc agggtaaagg cctgatgcca
     4381 gacggcacca cccgtttttc ttacaacggg cagccgcttt atcactatat ggggtgctct
     4441 acattcagtg aatacaccgt ggtcgcggaa gtgtctctgg ccaaaattaa tccagaagca
     4501 aaccatgaac acgtctgcct gctgggctgt ggcgtgacca ccggtattgg cgcggtacac
     4561 aacacagcta aagtccagcc aggtgattct gttgccgtat ttggtcttgg cgcgattggt
     4621 ctggcagtgg ttcagggcgc gcgtcaggcg aaagcgggac ggattatcgc tattgatacg
     4681 aacccgaaga aattcgatct ggcgcgtcgc ttcggtgcta ccgactgcat taacccgaat
     4741 gactacgaca aaccgattaa agatgtcctg ctggatatca acaaatgggg tatcgaccat
     4801 acctttgaat gcatcggtaa tgtcaacgtg atgcgtgcgg cgctggaaag tgcgcaccgc
     4861 ggctggggtc agtcggtgat catcggggtg gcaggttctg gtcaggaaat ctccacccgt
     4921 ccattccagt tggtcactgg tcgcgtatgg aaaggttccg cgtttggcgg cgtgaaaggt
     4981 cgttcccagt taccgggtat ggttgaagat gcgatgaaag gtgatatcga tctggaaccg
     5041 tttgtcacgc ataccatgag ccttgatgaa attaatgacg ccttcgacct gatgcatgaa
     5101 ggcaaatcca ttcgaaccgt aattcgttac tgatttcccg caggtatacc ccgtccactt
     5161 cagacggggt tcttaatact ctccctgggc agccgtccgg gggattaacc gtgagataat
     5221 gactgatgga actcattgaa aaacatgcca gctttggcgg ctggcaaaat gtgtatcggc
     5281 attattccca atcactgaaa tgtgaaatga atgtcggcgt ctatctccca ccaaaagccg
     5341 cgaatgaaaa attgccggtg ctgtactggc tttcgggcct gacctgcaac gagcagaatt
     5401 tcattactaa atcggggatg cagcgttacg cggctgaaca caacattatt gttgttgcgc
     5461 cggacaccag tccgcgaggc agtcatgtcg cagatgccga ccgttacgat ctcgggcaag
     5521 gtgccgggtt ttacctgaac gcaacgcaag cgccgtggaa tgaacattac aaaatgtatg
     5581 actatatccg caacgagctg ccggatttag tgatgcaaca ttttccggca acgaccagaa
     5641 agtctatctc tggtcattct atgggcgggc tgggcgcgct ggtgctggcg ttacgtaacc
     5701 cagatgaata tgtcagcgtc tcggcgtttt cgcccattgt ctccccatcg caagtaccgt
     5761 ggggacagca agcctttgcc gcatatcttg gtgaaaataa agatgcctgg ttgaattacg
     5821 atccggtgag tcttatttca caaggtcaac gcgttgcgga aatcatggtt gatcaggggt
     5881 tgagtgatga tttttacgcg gaacagctgc ggactccaaa tcttgaaaag atctgccagg
     5941 agatgaatat caagacgtta atccgttatc acgagggtta cgaccacagc tattattttg
     6001 tctccagttt tattggcgag catattgcct accacgccaa taaactgaat atgcgttgat
     6061 aatagtgcac gactgccgga tgcggcgtga acgccttatc cgcctacatt tcgcccgcag
     6121 gccgtaggca tgataagacg cgcaaagcgt cgcatcaggc atggcgcacg actgccggat
     6181 gcggagtgaa cgccttatcc ggcctacact tcgcccataa accgtaggcc tgataagacg
     6241 cgcatagcgt cgcatcaggc atctgcgcac gacttatcta tgtttaccac atcaaatcat
     6301 caggcacttt gaagtcggca tacgggtcgt cttcatcctg ctcttcggca ctgagcgcgc
     6361 tgtgcaacac aatactgcta gcatcacgct gagcaatttt atcggcaacg ctggcgggaa
     6421 taatggcgta ttcaccttcg ctattgttat cgaccaacaa gcgggcaatc gccagacgac
     6481 cattaatcaa ctgcgcttgt gtgagcttat cgacaaaaat cttcttaatc agattgccgt
     6541 cagtgaagtt aaaaccaata tcgccattgg caatggtgat tcggttcatt tcaatgagct
     6601 gcttcacctg agctttatat tctttcgcca acgccgcttg tttttgctgt tcgctaagct
     6661 gtttatcacg ctcaagctgt gcctttttat tttcttctac cgccgcccga gcttcacgcg
     6721 cctgaacgcg cgattttttc gccgttctct cgaccttcgc cgcttttttg ctggtgacta
     6781 atcctgcttt gagcaactgc tcttgtaagg taagttttgc catctttgtt tccaaacccg
     6841 ctgaataatt tttagcgagt atacctgtaa acgccgtggg tgtattgggg gcaagtccct
     6901 ttcgcccctg cttaataagg cgcgtcaggc atcggcacac ggctgcattc ttgatctccg
     6961 actcatcaaa ataaacaccg ccaacccagc aacaagaata cccggtgcag aagcggccat
     7021 tacgccgacc gtgccagtgc ctaatgccag cattttcccg gccagtaacg gaccgctcat
     7081 agcccccaga cgccctaccg ccacggctgt tcccacacct gttgcgcgga tctgcgaact
     7141 gtaaaacaac ggtgccaggg catacaaaac gctttgtcca cctgtcgcaa acaaccccgc
     7201 gacaaatccc gccagcaaca taccgttaaa tgacgacacc gttccaagcg ccagcagcga
     7261 agctaacatg ccgctataaa tcagtagcga catggttact ggacgcagct tatccatcaa
     7321 tgcgcccaac attaacgtcc cgcttgccgc ccccatttgc agggcaaaca tcacccctgc
     7381 cgcctgcgat ggctggaatc cttgctccac caaaagtagc ggtagccagt tgatcaacat
     7441 ataaaccacc agcagagtga agaaataaca caaccacagc agcagcgtcg cggttgccgt
     7501 ttctggcgca aataaggcac gcagtggtgg cgcagcctgt ttttcgccag cgaaaaccgc
     7561 tgactccggc agccagcgca ttaatagcgg caccagaatc aacggcacca caccacctac
     7621 ccaaaacacc gtttgccatg ctaagtttgc ccccgcgaaa cccagtgtcg ccgccagcgc
     7681 cgcgccaatg ggaacaccgc aatacatcag gctcactgcc gtcccacgaa aacgtggacc
     7741 cgcggcttca gacgtcaggg cgataagatt cggcaacgcc gcccccagcc cgacaccggt
     7801 catcagccgc gcaaagacca gtgaggggaa atcccaggca atcgccgttg ccagtgagaa
     7861 caaaccaaac agcgcaactg agccaatcaa aatacgctta cgaccataac ggtctgccag
     7921 catcccgcca accaacgcgc cgggtagcaa accgagtatt ccggcgctaa atatccagcc
     7981 catttgcatt ttatcgagtg cgaaagcctg ggcgatgcca cccgccgcaa tgccagccgc
     8041 ctgaagatcc agcccttcca tcagagcgac caaaaaacaa agcccgatgg tcagcatcag
     8101 gcgggatgaa gatgatgaag gggtacgagt cgacataaga tgaacctcat tgcgagtgca
     8161 ggtgaatcgc gactgataca ttgccagccg cgtaatcaga actatttaat gactgcctac
     8221 gggcgaaatg taggccggat aaggcgttca cgccgcatcc ggcagtcgtg ctgggatggg
     8281 catacaaaat gcacgttctc ggcagtcatc attatttgtt gttgcgcaga tccagcgcca
     8341 cgtcaacgat catatcctcc tggccgccaa ccatccggcg tttgcccagc tcaacgagaa
     8401 tatccaccgc acttaagcca taacgcgccg ccgccgtttc acagtgacgc aggaagctcg
     8461 agtaaacacc agcgtatccc agcgccagcg tttcgcgatc gactcgtacc ggtcgatcct
     8521 gcaacggacg caccaggtcg tcggcggcat ccattaacgc atagagatcg gtcccatgct
     8581 gccagcccag tttatccgcg gcggcaataa acacttccag cggtgcgtta cctgcgcccg
     8641 cgcccattcc cgcgaggctg gcgtcgattc ggtcgcagcc ctcttccacc gccgcgatag
     8701 agttcgccac gccaagactc aggttatggt gagcgtgcat gccagtttgc gtttctggtt
     8761 tcagctctgc tttcagggcg cggaaacggt cacggatatc gctcatgttc atcgcaccgc
     8821 cagaatccac cacataaata caggtcgcac cgtagccttc catcagcttt gcctgctttg
     8881 cgagattctc cggcgtggtc atatggctca tcatcagaaa accaacggtg tccattccga
     8941 gctcgcgggc atactgaata tgctgggcgg aaacatcagc ttcggtacag tgcgttgcca
     9001 cacgaaccac ccgcgcgcca gcctgccagg catttttcag atcgtgaata gtgccgattc
     9061 ctggcagcaa caacgtcgcg attttggcgt gcttcaccac atccgccgct gcttcaatcc
     9121 attcaaggtc gctatgtgcg ccgaaaccat agttaaagct ggaaccctgc aaaccgtcgc
     9181 catgggccac ttcaatcgaa tccacgcggg catcgtccag tgctttggca atctggcgaa
     9241 cgttttccag cgaatactga tgacgaatgg cgtgcatacc gtcacgcaac gtgacgtccg
     9301 agatataaag ttttttaccg ttcatgccgc ttctcctgcc ttgcgcgcca gtgactgggc
     9361 cattttttcc gctgtcgcca gcgcactgga agtcataatg tcgaggttgc ccgcataggc
     9421 aggcagataa tgcgctgcgc cttcgacttc cagccagacc gctgttttca gtccggagaa
     9481 ttgccccacg cccggtaaat tgaccggttt atcctgcggg ataacttcaa actgcacgcg
     9541 ctgtttcagg cgataacccg gtacgtaagc ctgcaccgcc tcagccattt cattgattga
     9601 ggcttcgata tcatcttgtg aagcttcgtc gctcaataca tacaccgtgt cacgcatcat
     9661 caacggtggc tctgctgggt taagcacaat aatcgccttc ccttttgccg cgccgcccac
     9721 cacttcaatg gctcgggaag tggtttccgt aaattcatcg atattggcac gcgtgccagg
     9781 tccggcagat ttactggcga tagaagcgat aatttcggcg taatgaacac gcgccacgcg
     9841 tgaaactgcc gccaccattg gaatggtggc ctggccgccg caggtgacca tgttgacgtt
     9901 cagttgatcg acgttcgcct cgaggttaac caccggcacg cagtaagggc cgatggcagc
     9961 aggcgtcagg tcaattaagc gaatatccgg tttcgcttcg cgtaaagcgg catcgttttt
    10021 cacatgcgcg cccgcgctgg tcgcatcaaa tacaatgtcg atatcagcaa attcaggcat
    10081 gttcatcagc ccgatcaccc cttcatgggt ggtggcgacg cccatacgtc tggcacgcgc
    10141 cagaccgtcg gactgaggat caatgccgac catcaccgcc atctccagat gctgaccgtg
    10201 acgcaaaatt ttaatcatca gatcggtgcc aatgttgcca gaaccgataa tggcgacttt
    10261 acgcttactc atgacagact tccttttggg gctgcgcttg aaaatgtcgc agcaactgaa
    10321 cctatgcctt caatatgggc ttcaaaacga tcgcccgcat tcaccgccac catcgggcct
    10381 aatgccccgg taagaatgat atctccggcg cgcagcggtt cacccagact ggccattttg
    10441 cgtgccagcc agacggcggc attaagcgga tgtcccaggc attcgctgcc gcgcccgctg
    10501 gaaacctctt cgttattacg cgtcatcttc atggcgcagt ttttcaggtc taaccccgcc
    10561 ggacgttgcg ccggaccgcc gatgacatac accccgcagg aagcgttatc tgccacggta
    10621 tcgacaaact gaatcgacca gtcgcgaatg cggctcccca ccacttccag cgccggaagt
    10681 acccattcaa tggcgttata cagttcgtcg aaggtgatat cagttgcggg taaatcgcgg
    10741 ttcaacacca gtgcgatctc cgcttcaatg cggggttgca gaacacggga aaaaggaatg
    10801 atttcgttat cgccataaca catgtcggca aataacgtcc caaaatccgg ttgatcaacg
    10861 cccagttgtt gttgcacttt cggatgcgtc aggcccactt tacgccctac cacgcgacgc
    10921 ccctgcacaa cgtcatattg cacatttatg tgctgaatgg cgtaggccgc ttcagcgtta
    10981 tcgataccaa tcagatcgcg cagcggcgca atcgcttcgc cctgctctgc ggcgcggcgt
    11041 aaatccgccg ccagttgctc aagagtatgc ttcgtcatga ccattcctta agggcgtgcg
    11101 aggaaattca gcaccaattg attgaaagcg tcggcatgtt cccactgcgc ccagtgacca
    11161 cagtcgcgga agatatgcag ttcagaaccg gcaatgccgg acagcagacg cagacccgca
    11221 tccatcggca caaagcggtc gttgcgcccc cagacaatca gggtttgcgc tttgatttcc
    11281 gccagacgtg ggccaaaatc cgggaactgt ttcggattag cttccaggct cttaacgaag
    11341 ttttccaggt gatcgcggcg cgacagcata ttattcaggc gcgcttcaaa cagggcgtcg
    11401 gtcaaatcgc tggtatcgaa aacgaagata tccatcatca gcttcaggtt ttcgatagtc
    11461 ggctgacgat aaagctgatt cagtcgctta ataccttcgg ttggcatcgg cgtaaacaaa
    11521 ctcatgccgc ccgtaccgcc gcccatcagc accagtttgc cgacgcgctc cggccagtta
    11581 agggtgaacg ccacagaact atggccgccc atcgagttgc ccagcaggtg gattttggcg
    11641 atatccagtt gatccaccac gcttttcagg attcgtgcat taagatccga tcgtgaacca
    11701 ctattaacga tcgaatcgct tttgccccaa cccggacaat ccagcaggat tacccgatag
    11761 cccgcctcta ccagcggatc gatattgcgg ctgaagttcg cccagccagt agcacccggg
    11821 ccggaaccat gcagcaggac aacggtttcg tcgccttgtc cgcagtcatt aaaatggatg
    11881 cgcagcgttt tacccgcttc ttctacattc agaaaacggc tggtggcggc ttcggtttgt
    11941 ggctgataac tcatcatctt ctcctgcata ttcagttctc tgttctggcg cttaacgagc
    12001 caaatccggc aatccactcc gggattgggc ggtaataacg cccttcgcta cgccagttgc
    12061 caaacgcaga aatagcggca aaagcggcga cccaggtttt gatttcatgt gtcgactttc
    12121 cggcaatggc ggaaagctct tcgttactga cggcatccag ttcctgtatg cgtccctgct
    12181 ccagcaaagt catgaactgg ttatcccaaa tcgggttgag cggatgcaga gttctctgat
    12241 cctcaacaaa cttctcagcg gcgctaatca cccgttgctg acgcaattcg cgctcactgg
    12301 cgggtaaatc tttcccgctc cccaacagac ggtcgcgcat atgggcatcg gctttcgcca
    12361 gttcgggcac cggcggctga tgggaaagcc caccggaacc caggaacagc acgcgtttat
    12421 tgagagtgct ggtgaaacgt ccaatggctt cacccaacat gcgggtacgc tggaaaccgg
    12481 gcagcggcgt ggcgacaccg ttgatgaaca caggcagaac tggcacctta tccagcccac
    12541 cgagcaggaa ctccagcggc tgggcgaacc cgtggtccac ctgcatacag taagaaacgg
    12601 caagatcgat cccgctcttc atgacagcat gcgcacaggc ctccgccagc tccacaggca
    12661 cgggcagctc tcctgccgca ctgccgaaat caccaattgc cgtcgctcca acgcctaaac
    12721 agaacggtgg catcacgtca tagaaaaagc cgttgtagtg atctggcgca aacagcacca
    12781 ccagttcagg ggagaaagcc gcaatacgct cgcgggcgct ggcaatcacg ccattgacct
    12841 catcgagtac ctcttgcgcc gggtcgacat accccaccaa cggcgagtgg gaaagacagt
    12901 gaagataagc gtgcatatca ggctaccttt tcgacagaaa cgtcggcatc agggcgggtc
    12961 agcgtcatca ccgacgccag tttattcagc gtattgccca gggtttgcgg aatggcggtg
    13021 gcggcaacaa agcgatccgg gcgcatcacc accagcgaag cattgtgttg cgcgaaccag
    13081 ctacgcagac gaccttgcgt atcgcccacg cgtagcacgc cgtcgtggtt atcctgtgcg
    13141 gtatgaattt gcacttccgg caccacctga atgaagcgtg tgcccaacgc gcgccactgc
    13201 tggatttgct cgtcgctcat cccccacagt ggattgcatc cccagccaat taccgcgaag
    13261 ttcgcgccaa tcgcgttatc gagcagcgtc acgtcgccgt tttccagcgt gactttcggc
    13321 tgaataaaca tcttgccgac cggagagtgc ttcgcttcgc cctcacgtac cagcgcaccg
    13381 ccgtaatatt gcggcatcgg cttgaagcgc atttcgagga agtagcgttt tactggcggc
    13441 agataattca gtagccagga aacgccgtca cgtaacgtac cctgccagcg tttcggcgga
    13501 gccagcacgt tgcccgccgt cacggacagg tcaatcatcg ctttggcgtg atcgcgacgt
    13561 tcttgttgat aggtatcgag cagcgcatcg cgggctttcc cctggataac caacgccagt
    13621 ttccaggcga ggttaaaggc gtcgcgcata ccactgttat agccctgccc ctgccacacc
    13681 ggcatgatgt gcgcggcatc gcccgccagc agtacgcgat caatacggaa acgttgcgcc
    13741 agtcgcgcgt tatgggtgta gacacgctgg cgaatcaatt caacattgtc cggattaggc
    13801 agcactttgc ttaacagctt gcgcatattt tgcggctcac gcagctgctc ttcggtttct
    13861 cccggcatca ccataaattc aaagcgacgt accgcatgag gcagcgcggc agaaacatac
    13921 gggcgcaccg gatcgcaaca caaatagata tgcggcgtac ttaacggatc gttggcgata
    13981 tctaccacaa tccactgatt tggcgcagtt ttaccttcaa acggcacatt cagagtgcga
    14041 cggacaaaac ttgctccacc gtcacaggct accagccact gggctttgac tatttcccgc
    14101 tgcccttctg ccgttttcag gtgcaaggtc acttcgtcat tttgctggct gaaggcctcc
    14161 agctcgcggg aaaacaagca gcgcacatta ggaaaacgcg acaccccttc cagcatcacc
    14221 gcatcgacct gcggctgaat aaaggcgtta cggcgcggcc agccaaattc atcggtcatt
    14281 ggctgaatat cagcaaaaca gcggcctttc ggggtgagaa aacgcatcgc gtgccacggc
    14341 gtagtgtgcg gcagaacatt ctcgaccagg ccaaccgact gcatggtgcg cagcgcctcg
    14401 tcatcaatac caatcgcacg cgggtagtcg atcaacttat cgagtttctc caccaccagc
    14461 acgtcaatgc ccatctgacc gagatagttc gccatcatca gcccgaccgg accggcacct
    14521 gcgatcgcca cctgaacgct atggttaaca gcaggctgga tgtcagggtg ttgtattgcc
    14581 atttcagtac ctcacgactc ggacaaaatg ccgttgcgcg cacagtacgg cgcaacttat
    14641 tttgttaaaa acatgtaaat gattttttat tgtgcgctca gtataggaag ggtgttttcg
    14701 gctacaatca aaacatgccc gaatgtgcac caggtgcaca acgttgtttt aactatagaa
    14761 atgtcaatta atatgcagaa caatgagcag acggaataca aaaccgtgcg cggcttaacc
    14821 cgcggtctaa tgttattaaa tatgttaaat aaacttgatg gcggtgccag cgtcgggctg
    14881 ctggcggaac tcagcggcct gcatcgcacc actgtgcggc gactgctgga gacgctgcag
    14941 gaagagggat atgtccgccg tagcccctcc gatgatagtt ttcgactgac catcaaagtg
    15001 cggcaattaa gcgaaggatt tcgtgacgaa cagtggattt ctgcactggc ggccccactg
    15061 ctgggcgatc tgttgcgcga agtggtatgg ccgacagatg tgtccacgct ggatgttgat
    15121 gcaatggtgg tacgcgaaac cactcaccgt ttcagccgct tatcctttca ccgggcaatg
    15181 gtcgggcgac gtttgccgct tctgaaaacc gcctcgggcc tgacctggct ggccttttgc
    15241 ccggaacaag accgcaagga attaatcgaa atgttagcct cccgccccgg tgatgactat
    15301 caactggcac gggaaccgtt aaagctggaa gccattctgg cgcgcgcgcg caaagagggt
    15361 tacggacaga actaccgcgg ctgggatcag gaggagaaga tcgcctctat cgccgtaccg
    15421 ctgcgcagtg aacaacgggt gattggctgt ctgaatctgg tgtatatggc gagcgcaatg
    15481 accattgaac aggcagcgga aaagcatctt ccggcgctac aacgggtagc aaaacagatc
    15541 gaagaagggg ttgaatcgca ggctattctg gtggccggac ggcgaagcgg cgtgcattta
    15601 cgttgacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gtccggaaga
    15661 gagtcaattc agggtggtga atgtgaaacc agtaacgcta tacgatgtcg cagagtatgc
    15721 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
    15781 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
    15841 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
    15901 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gctgatcaac tgggtgccag
    15961 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
    16021 tcttctcgcg caacgcgtca gtgggctgct cattaactat ccgctggatg accaggatgc
    16081 cactgctgtg gaagcagcct gcgctaatgt tccggcgtta tttcttgatg tctctgacca
    16141 gactcccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
    16201 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
    16261 ggcgcgtctg cgtctggcgg gctggcataa atatctcaca cgcaatcaaa ttcagccgat
    16321 agcggaacgg gagggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
    16381 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
    16441 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata
    16501 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
    16561 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
    16621 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa
    16681 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    16741 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    16801 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgaa attgtgagcg
    16861 gataacaatt tcacacagga aacagctatg tccatgatta cggattcact ggccgtcgtt
    16921 ttacaacgtc gtgactggga aaaccctggc gttacacaac ttaatcgcct tgcggcacat
    16981 ccccctttcg ccagctggcg taatagcgaa gaagcccgca ccgatcgccc ttcccaacag
    17041 ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg
    17101 gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg
    17161 cagatgcacg gttacgatgc gcccatctac accaacgtga cctatcccat tacggtcaat
    17221 ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt taatgttgat
    17281 gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttaa ctcggcgttt
    17341 catctgtggt gcaacggtcg ctgggtcggt tacggtcagg acagtcgttt gccgtctgaa
    17401 tttgacctga gcgcattttt acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgc
    17461 tggagtgacg gcagttatct ggaagatcag gatatgtggc ggatgagcgg cattttccgt
    17521 gacgtctcgt tgctgcataa accgactaca caaatcagcg atttccatgt tgccactcgc
    17581 tttaatgatg atttcagccg cgctgtactg gaggctgaag ttcagatgtg cggcgagttg
    17641 cgtgactacc tacgggtaac agtttcttta tggcagggtg aaacgcaggt cgccagcggc
    17701 accgcgcctt tcggcggtga aattatcgat gagcgtggta gttatgccga tcgcgtcaca
    17761 ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg aaatcccgaa tctctatcgt
    17821 gcggtggttg aactgcacac cgccgacggc acgctgattg aagcagaagc ctgcgatgtc
    17881 ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg
    17941 attcgaggcg ttaaccgtca cgagcatcat cctctgcatg gtcaggtcat ggatgagcag
    18001 acgatggtgc aggatatcct gctgatgaag cagaacaact ttaacgccgt gcgctgttcg
    18061 cattatccga accatccgct gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg
    18121 gatgaagcca atattgaaac ccacggcatg gtgccaatga atcgtctgac tgatgatccg
    18181 cgctggctac cggcgatgag cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac
    18241 ccgagtgtga tcatctggtc gctggggaat gaatcaggcc acggcgctaa tcacgacgcg
    18301 ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga
    18361 gccgacacca cggccaccga tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag
    18421 cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc tggagagacg
    18481 cgcccgctga tcctttgcga atacgcccac gcgatgggta acagtcttgg cggtttcgct
    18541 aaatactggc aggcgtttcg tcagtatccc cgtttacagg gcggcttcgt ctgggactgg
    18601 gtggatcagt cgctgattaa atatgatgaa aacggcaacc cgtggtcggc ttacggcggt
    18661 gattttggcg atacgccgaa cgatcgccag ttctgtatga acggtctggt ctttgccgac
    18721 cgcacgccgc atccagcgct gacggaagca aaacaccagc agcagttttt ccagttccgt
    18781 ttatccgggc aaaccatcga agtgaccagc gaatacctgt tccgtcatag cgataacgag
    18841 ctcctgcact ggatggtggc gctggatggt aagccgctgg caagcggtga agtgcctctg
    18901 gatgtcgctc cacaaggtaa acagttgatt gaactgcctg aactaccgca gccggagagc
    18961 gccgggcaac tctggctcac agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa
    19021 gccgggcaca tcagcgcctg gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc
    19081 ccctccgcgt cccatatcat cccgcaacta accaccagcg aaacggattt ttgcatcgag
    19141 ctgggtaata agcgttggca atttaaccgt cagtcaggcc ttctttcaca gatgtggatt
    19201 ggcgatgaaa aacaactgct gacgccgctg cgcgatcagt tcacccgtgc accgctggat
    19261 aacgacattg gcgtaagtga agcgacccgc attgacccta acgcctgggt cgaacgctgg
    19321 aaggcggcgg gccattacca ggccgaagca gcgttgttgc agtgctcggc agatacactt
    19381 gccgacgcgg tgctgattac gaccgctcac gcgtggcagc atcagggaaa aaccttattt
    19441 atcagccgga aaacctaccg gattgatggt agtggtcaaa tggcgattac cgttgatgtt
    19501 gaagtagcga gcgatacacc gcatccggca cggattggcc tgacctgcca gctggcgcag
    19561 gtcgcagagc gggtaaactg gctcggatta gggccgcaag aaaactatcc cgaccgcctt
    19621 actgcggcct gttttgaccg ctgggatctg ccattgtcag acatgtatac cccgtacgtc
    19681 ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat tgaattatgg cccacaccag
    19741 tggcgcggcg acttccagtt caacatcagc cgctacagtc aacagcaact gatggaaacc
    19801 agccatcgcc atctgctgca cgcggaagaa ggcacatggc tgaatatcga cggtttccat
    19861 atggggattg gtggcgacga ctcctggagc ccgtcagtgt cggcggaatt ccagctgagc
    19921 gccggtcgct accattacca gttggtctgg tgtcaaaaat aataataacc gggcaggcca
    19981 tgtctgcccg tatttcgcgt aaggaaatcc attatgtact atttaaaaaa cacaaacttt
    20041 tggatgttcg gtttattctt tttcttttac ttttttatca tgggagccta cttcccgttt
    20101 ttcccgattt ggctacatga catcaaccat atcagcaaaa gtgatacggg tattattttt
    20161 gccgctattt ctctgttctc gctattattc caaccgctgt ttggtctgct ttctgacaaa
    20221 ctcgggctgc gcaaatacct gctgtggatt attaccggca tgttagtgat gtttgcgccg
    20281 ttctttattt ttatcttcgg gccactgtta caatacaaca ttttagtagg atcgattgtt
    20341 ggtggtattt atctaggctt ttgttttaac gccggtgcgc cagcagtaga ggcatttatt
    20401 gagaaagtca gccgtcgcag taatttcgaa tttggtcgcg cgcggatgtt tggctgtgtt
    20461 ggctgggcgt tgtgtgcctc gattgtcggc atcatgttca ccatcaataa tcagtttgtt
    20521 ttctggctgg gttctggctg tgcactcatc ctcgccgttt tactcttttt cgccaaaacg
    20581 gatgcgccct cttccgccac ggttgccaat gcggtaggtg ccaaccattc ggcatttagc
    20641 cttaagctgg cgctggaact gttcagacag ccaaaactgt ggtttttgtc actgtatgtt
    20701 attggcgttt cctgcaccta cgatgttttt gaccaacagt ttgctaattt ctttacttcg
    20761 ttctttgcta ccggtgaaca gggtacgcgg gtatttggct acgtaacgac aatgggcgaa
    20821 ttacttaacg cctcgattat gttctttgcg ccactgatta ttaatcgcat cggtgggaaa
    20881 aatgccctgc tgctggctgg cactattatg tctgtacgta ttattggctc atcgttcgcc
    20941 acctcagcgc tggaagtggt tattctgaaa acgctgcata tgtttgaagt accgttcctg
    21001 ctggtgggct gctttaaata tattaccagc cagtttgaag tgcgtttttc agcgacgatt
    21061 tatctggtct gtttctgctt ctttaagcaa ctggcgatga tttttatgtc tgtactggcg
    21121 ggtaatatgt atgaaagcat cggtttccag ggcgcttatc tggtgctggg tctggtggcg
    21181 ctgggcttca ccttaatttc cgtgttcacg cttagcggcc ccggcccgct ttctctactg
    21241 cgtcgtcagg tgaatgaagt cgcttaagca attaatgtcg gatgcgccac tgggcacatc
    21301 cgacaattat ttatagctcc atcatctggc gaaacaagcg tcgcatcata cctctttgac
    21361 ccacagctgc ggaaaacgtg ctggtgcaaa acgcagggtt atgatcatca gcccaacgac
    21421 gcacagcgta tgaaatgccc agtccatcag gtaattgccg ctgatactac gcagcaaacc
    21481 agaaaaccac ggggcaagcc cggcgatgat aaaaccgatc ccctgcataa acgccaccag
    21541 cttgccagca atagccggtt gcgcagagtg atcgagcgcc agcagcaaac agagcggaaa
    21601 cgcgccgccc agacctaacc cacacaccat cgcccacaat accggcaatt gcatcggcag
    21661 ccagataaag ccgcagaacc ccaccagttg taacaccagc gccagcatta acagtttgcg
    21721 ccgatcctga tggcgagcca tagcaggcat cagcaaagct cctgcggctt gcccaagcgt
    21781 catcaatgcc agcaaggaac cgctgtactg cgcgctggca ccaatctcaa tatagaaggc
    21841 gggtaaccag gcaatcaggc tggcgtaacc accgttaatc agaccaaagt aaacacccag
    21901 cgtccacgcg cgaggagtga aaaccacgcg aaccggagtg gttgttgtct tgtgggaaga
    21961 ggcgacctcg cgggtgcttt gccaccacca ggcaaagagc gcaacaacgg caggcagcgc
    22021 ccaccaggcg agtgtttgat accaggtttc gctatgttga actaaccagg gcgttatggc
    22081 ggcaccaagt ccaccgccgc ccatcagagc cgcggaccac agccccatca ccagtggcgt
    22141 gcgctgctga aaccgccgtt taatcaccga aggcatcacc gcctgaatga tgccgatccc
    22201 caccccacca agcagtgcgc tgctaagcag cagcgcactt tgggggtaaa gctcacgcat
    22261 caatgcaccg acggcaatca gcaacagact gatggcgaca ctgcgacgtt cgctgacatg
    22321 ctgatgaagc cagcttccgg ccagcgccag cccgcccatg gtgaccaccg gcagagcggt
    22381 caacagggca gccacgctaa agctcattcc gctcgcctgg cgcaattgcg gtagcagtgg
    22441 cccgacggag gtgagcagtg gtcgcatatt aagaccaatc agcaccagaa ccagcagcat
    22501 cgtgaacgtc ttttgatgat tgcgcatggc tgtcagaacg gcttggtcgg cagatattta
    22561 ccatctaagg taatgaccgc acgttcgcca ccttccgggt ccgccacttt cttaacgtcg
    22621 agtttgaagt taatcgcgct gataatgcca tcgccaaact tctcatgaac caacgctttc
    22681 agggttgtgc cgtacacctg caacatttca tagaaacgat acatcgttgg gtcggttgga
    22741 atacggtcat caatgcagcc acgcagtgga atcatctgca acagtagaat ggcgtcttcg
    22801 tcgagatcca gcttcacccc gaccaggcgg gcggcgtcgg caggaagcgc ttgctgcccc
    22861 agcaaagccg cggttacgaa ggcttctgcc agaccggtac cgtcggcaat ctcggcaaat
    22921 gagagatctt ttttagcttt gctgagcaaa atggcatcgg caagatcaag acgaatattg
    22981 cggttaattt gtgactgaat catggtggat ctcctgatgg tttaaaaata aggttacgct
    23041 gcggtcggtt ggcgtagcgg tatggcacaa acgcgaggat tagcggccag tggcacaaac
    23101 tggcgggttg cgccgtcaaa agctgcgatg ctgccgcttt caatgtcgta gacccagccg
    23161 tgcagggcga tccgcccctc ttcgagcgcc aggcgcaccg atggatgagt ttgcaaatta
    23221 gccaactgag caatgacgtt ttcacgtacc atcgccgcag cttttgacgg taaatcggaa
    23281 tgcgggcgcg cctcattaac gacgcgggct gaatcggcat aacgcagcca gtgggagacg
    23341 gcaggcatat ggtccatgca ctgacagctg gcaatggcgg tcatcgcgcc acagttggaa
    23401 tgaccacaaa tcacaatgtc agatacccga agcgcagcga cggcatactc caccgaagca
    23461 gaaacgccac cgggttccgg cccgtaggaa gggacgatat tgcccgcgtt gcgaataacg
    23521 aacagatcgc caggctcacg ttgcgtcacc agctcaggga ccagacggct gtcggagcag
    23581 gagataaaaa gtgtgcgcgg gctttgctgt gtcgccagct gtttaaacaa ggcttcccgc
    23641 ttcggaaatg cctcgcgctg gaatttaagg aatccatcaa taatctcttt caccgttaac
    23701 ctctgtctgt ctctggaatg agaggcagac tactcgccga ttgtcataag gtaaaagtct
    23761 catttatgat gagttctatt ggatttactt ataggttggc gaatgctctc tcgacatatc
    23821 aactattttc ttgccgtggc tgaacatggc agctttaccc gtgccgccag tgcgttgcac
    23881 gtctcccagc ctgcgctttc ccagcagatt cgccagttag aggagagttt aggcgtgccg
    23941 ctgtttgacc gtagcgggcg aacgattcgt ctcactgatg caggagaagt ctggcgacag
    24001 tacgccagcc gggcgttaca ggaactgggg gcgggtaaac gggcgattca tgatgttgcc
    24061 gatctgacgc gaggatcgct gcgtatcgcc gtcactccca cctttacaag ctactttatc
    24121 ggccctttaa tggcggattt ctatgcgcgc taccccggca tcacgctcca gctacaggaa
    24181 atgtcgcagg agaaaatcga agatctgctt tgtcgcgacg agctggacgt tgggattgcc
    24241 ttcgcgcctg tgcattcgcc ggagctggag gcaattcctt tactgacaga aagtttagcg
    24301 ttggtggtgg cgaaacatca tccgctggct gcctgtgaac aggtggcgtt gagtcgcttg
    24361 catgatgaaa aactggtcct gctcagcgcg gaatttgcca ccagagagca aattgaccac
    24421 tactgcgaga aagcggggct acatccacag gtggtcattg aggcgaactc aattagcgcg
    24481 gttctggagc tgattcgccg cacttccctt tccacattgt taccagcagc gattgccaca
    24541 caacatgacg ggcttaaagc gatttctctt gccccgccac tactggagag aacggcggtt
    24601 ttgttgcggc ggaaaaatag ctggcagaca gccgccgcga aggcattttt gcacatggcg
    24661 ttggaggaat gtgcggatgt tggcgagaat gaatcacggt agccactgac acaggtcagt
    24721 ggctcttcaa cacagaggaa attaccgttt gtaatcgatg gcttctggct gctccagata
    24781 tacggtggtt tgtgccggtt gtgtactggc aatcaccttg ccgccacgta ccgaataacg
    24841 taccggaacc tgacggcgca gcgcatcaaa tccattttca gccggcagga taatcaggtt
    24901 tgcgctgttt ccggcggcaa tgccgtaatc ctgcaaattc aatgttctgg cactgtggtg
    24961 ggtgattaaa ttcaggccat cattaatctg cccgtagccc atcaactggc aaacatgcag
    25021 ccccatatgc agcacttgca gcatattcgc cgttcccagc ggataccacg gatcgaagac
    25081 atcatcgtga ccaaagcaga cgttaatgcc ggactccagc atctctttaa cgcgcgtgat
    25141 gccgcgacgt tttggatacg tatcgaaacg tccttgcaga tgaatattga ccagcgggtt
    25201 ggcgacaaag ttaataccgg acattttcag caagcggaac aggcgtgagg tatacgcccc
    25261 gttataggag tgcattgccg tggtgtggct ggcggtgact cgcgcgccca tgccttcacg
    25321 gtgcgccagg gcagcaacgg tttcgacaaa gcgcgactgc tcgtcatcga tctcatcaca
    25381 gtgaacgtcg atgagacggt cgtatttttg cgccagggcg aaggttttat gcagcgactc
    25441 cacgccgtat tcacgggtaa attcaaaatg cggaatcgcc ccaacaacat ctgcccctaa
    25501 gcgtaacgcc tcttccagca acgcttcacc gttgggatac gacaaaatcc cttcctgagg
    25561 gaaggcgacg atttgcagat caatccacgg cgcgacttcc tgcttcactt ccagcattgc
    25621 tttcagcgcg gtcagggttg catccgaaac atcgacatgg gtacgcacat gctgaatgcc
    25681 gttggcaatc tgccatttca gcgtttgcca tgcgcgttgt ttcacatcgt catgggttaa
    25741 taacgctttg cgctcggccc agcgttcaat gccttcaaac agcgtgccgg actgattcca
    25801 gttcggttgt ccggcggttt gcgtggtgtc caggtgaata tgcggctcca caaacggcgg
    25861 tataactaaa ccttgttcgg catccaggct gttttcagtt atgggcatca cgccggattg
    25921 cgcatcaatg gcgctgattt ttccgtcctg cagatgaatc tgccacagcc cctctttgcc
    25981 tggtaaccgg gcgttaataa ttgtttgtaa agcgttattc gacactgtta acctccacat
    26041 gcgtcattgc tgctgtcgtt ttacgattca aaatcgggtt aaggatcaga tagctcagcg
    26101 cgccacctaa taccgcgttg accggaacaa ttcccggtaa ccagtggcct gcggcaatcc
    26161 ccagcgcgac cgccagaatc gccacccaat tgacactcat catacgcgtg gtcgcaaagt
    26221 gctcatagcg gcgacggttc atcagatagt cggcgatgat cacgccacct accggaggaa
    26281 tagctgccga aaggaaggtc aaccagccga caaaattgtt atacagccat aatgcgcaga
    26341 ccgtaccgat aataccgttg attaccgaaa gggttttgct cgacataccg gtaatgttgg
    26401 cgaaacctaa acccgacgca tagagtgcgt tatcgttggt ggtccagata ttcagcccca
    26461 gcaccacaat cgcaggcagc agcaggccct gagcaatcat cacatcagag atatccgcca
    26521 tgcccagtgc cgcagcgccc gctgcaccga aaataaacat caacgagttg ccgaggaaaa
    26581 aggccaccat cgccaccagc accgccagtt tggcattgcg accaaaccgg acaaagtcag
    26641 cggtgagcgt acccgcactg ataaatgacc ccacaaccag cgccagcgcg acattgaaat
    26701 ctaacggttg tgcgggaacg accgctttta atgcgtccag gccgcccatg ccgttaacag
    26761 ccagccacac ggaataaccg cccaggcagg cgatagccgg aaccgcaatc accgaaagaa
    26821 ccgtcagcgc cgaaatacca aaaaagacgg tgacggtcat cagtaaaccg gaaacggcaa
    26881 tcagcaaatt aatatccagc ccggttgcct tacccaccgg aatggcaaac atcgccacac
    26941 cgacgccaaa ccagccaacc tgagttccgc ccagtagcag tgaaggcagc catgagcctt
    27001 taacaccaaa cgagaagcga gcaagaagat gagtggtcag gccggttttt gcgccaatgt
    27061 aaccgagaaa tgaagtgtaa ataccgagga gaagattacc gatgagaact gcgaggaaga
    27121 aatcatgata gctaagaccg gttccgagag taccgccggt ccacatactg gcggaaaaga
    27181 aggttaatcc cagcatgacg aacgtcaatg ccaatacccc tttccgcgcc gactgcggga
    27241 ctggcccctg gctaaagttg ttatcttgcg acacgaaatt cccccttttt gctgtttgaa
    27301 acccaaaaaa tccgccgcat tctattcatc tgaagataaa aagcaatcgt tttcgtgggg
    27361 aaatatattt tttatatgaa tgagtgcaaa aaggtggggg aaaaattatc ggtgtaaaaa
    27421 cagtgggtag gcataataag acgcggtaag cgtcgcatca ggcatcggcg catggtgtcg
    27481 gatgcgccac gcgacgcatc cgacaatacc ttactcttcc atcgcctggc ggatctgatc
    27541 caacgacgca ggatcatcaa tggtcgtcag atccccagga tcgcgtcctt cgcaaatcgc
    27601 ctggatcgtg cggcgcagca tttttccgga tcgcgttttt ggcaattgcg agacaaacca
    27661 gacgtgcgcc gggcggccaa agttgccaat ctggctgtcc accagcgcca taatcgcctt
    27721 ctcttgcgag tgcgccacat cacgatcttc cagactgtcg ctctctttcg gaatgacaaa
    27781 cgccaccgcc acctgccctt tcagcgcatc tttcacccca accaccgcca cttcggcaac
    27841 gcccggatgg ctggagatac tctcttcaat ctcgcgcgtc cccagccgat gcccggcaac
    27901 gttaattaca tcgtcagtgc gcccgagaat aaagtgataa ccgtcagcgt cacggatgcc
    27961 ccagtcaaag gtggcgtaca ccgggcggga aaacagcgac cagtaagtct tcacaaagcg
    28021 gccgtcgtcg ccccagatgg tctgaataca ccccggcggc agcggccctt ccaccaccag
    28081 catccctttc tcgttgacgc cacacggttc gccggtgact tcattaagca actgcacgtt
    28141 atagccatac atcggcacgc cggggcttcc cagacgcgtc ggcctgtcgt ccagaccgcg
    28201 agcaatcgcc ataatcggcc agccggattc ggtctgccag tagttgtcga tgaccgacac
    28261 atccagcgta ttgctcaccc aactggcggt cggctcgtcc agcggttctc cagccagata
    28321 gagcacttcc agcgacgaga gatcgtgttt acgaatttca gcggtaggga attttttcag
    28381 cacgcgaata gcggtcggcg ctgagaacat ccggctaacc tgatatttct cgacgatttt
    28441 ccaccacacg ccgcagtccg gccaggtcgg caatccttcg taaacgatag tcgccatccc
    28501 cgccagcagc ggcgcgtaaa cgatatacga atgccccacc acccagccga tatccgatgc
    28561 gcaaaagaac acgccgcccg ctttgccgcc aaaaatggtg tccatcgagg tcgccagcgc
    28621 caccgcatat ccgccgacgt cacgctgcac gcctttaggt ttgccggtcg tgccggaagt
    28681 gtagagaatg caggaggttt cgttggattc cagccacgcc actggtaccc gcgcgccgat
    28741 gtgttgatag cgcaacgacg cgaaatcgac atcccgcccg ctgacgcgcg ccattttcgc
    28801 cagtccgcga tccaccagca aaacatggcg tggctggtgc tgcgcctgac ttatcgcatc
    28861 gtcgagcaat tttttatagg gaatgatttt gccaccgcgc gctccggcat cagccgaaac
    28921 aatcagcacc ggtttagcat catcaattcg cgccgccacg ctgtgcgagg caaatccacc
    28981 aaacaccacc gagtgaatag cgccaatgcg cgcgcaggcc agcagagtaa tatgcgcttc
    29041 ggcaatcatc ggcatataca ccagcacccg atcgccacgc tgcacgccca atgaacgcaa
    29101 cattgaggcc accgcgttta cttcgtcatg aagctgacga aaggtaaagg tgcgctcttc
    29161 ttctgtttcc gaagagacgg caatcagcgc cagcgcctct ggttgtttct ccagccagcg
    29221 gtcgatagcg ttgtggcaca agttggttcg gccttcacaa aaccaacggg caaacggcgg
    29281 attgctgtga tcgagcgttt gcgtaaaggg cgtctgccag tcaatacgcc gggcctgctc
    29341 ggcccagaac tgctccggtt cgttaatcga acgctgataa aattcgctaa aagacataat
    29401 gctctcctgt tgaacttacg ccttaccgct gtttgcttaa atgacgtaca ggtcgagata
    29461 ctcattgacc ggcatctgtt ccaggcgagt tctgtcgaga gaaacctcca gaatgcgctg
    29521 ctgttggcga gtcgggaact ggcgcgcgag attgattttg aatttatcga ccagtttcgg
    29581 aataccatcc tgacggcggc gagcatgacc aatggggtac tccaccacca cttcttcaaa
    29641 tcgtgtgccg tcggtgaact caagggtaat ggcattggcg atggcgcgtt tttccgggtc
    29701 gtggtagtca gcggtaaatg ccggatcttc aaagcaattg atcttctcgc gcagggcgtc
    29761 gatacgttta tcttgcgcca cgttgtcctc gtaatctgcc gccgttaagc gcccgaacag
    29821 cagcgggatc gccaccatgt actgaatgca gtgatcgcgg tctgccgggt tattgagcgg
    29881 tccttttttg tcgatgatgc gaatacaggc ttcgtgggtg cgaatggtca ctttttcgat
    29941 atccgccgcc gttttgcctg ctgcctgcat ctgttcatag agcgtcatcg ctgcttcaac
    30001 tgccgtctgg gagtggaact ccgccgggaa ggagattttg aacagcacat tttccataac
    30061 gtaggaaccg tacgggcgct ggaagcggaa cgattcacct ttaaaggaga cgtcgtagaa
    30121 gccccacacc ggcgcagtca gggctgacgg gtaacccatt tcgcccgttt tcgccatcag
    30181 tgccagacgt accgcgcggg aagtggcatc gcccgccgcc caggatttac gcgtgccggt
    30241 gttcggcgca tggcgatagg tgcgcagcga ctgaccgtcc acccacgcca gcgaaacggc
    30301 gttgagaatt tcctcgcggg tcaggccgag catttcggcg accacggcgg tggaagccac
    30361 tttcactaac agaacgtggt cgaggccgac gcggttaaag gagttttcca gcgcgatgca
    30421 gccctgaatt tcatgggctt tgatcattgc ggtcagcacc tgtttcatgg tcaacggcgc
    30481 tttgccgctg gcgaccgcgt tgcgcgaaag ccagtccgcc gttgccagaa tgccgccgag
    30541 gttgtcggaa ggatggcccc actccgccgc cagccaggta tcgttgaaat cgagccagcg
    30601 gatcatcgcg ccgatgttaa atgccgcctg gacggggtcg agctggaact gagttccggg
    30661 gacgcgcacg ccgttgggta cgacggtgcc gggaacaatt ggccccagca gttttttaca
    30721 ggccgggtat tcgagagctt ccagaccgca gccgagcgtg tcgagcaggc agtaatgtgc
    30781 ggtgtcgtag gccactttag agctgatttc gtagttcatg acgtaatcga cgatatcaac
    30841 gatttcacga tcaaattccg ggcggatgtt gttgatttga gctgacattg ggtacgtttc
    30901 cttattgtta ttcgtagagg tttactggcg cttatccagc gcgacaaacg ggcggtcttc
    30961 cggtccaaca taattggcgg aaggacggat aattttgttg tcctgacgtt gttcgataat
    31021 gtgcgccgcc cagccggtga cgcgggcgat aacaaacagt ggtgtaaaca tctcggtggg
    31081 aacgcccatc atgttgtagg aaacagcaga gaaccagtct agattgggga acatcttttt
    31141 gctctcccac atcaccgttt ccaggcgatc ggcgatgttg tacatcttca gcgagccgcc
    31201 ttcctgcgag agctgcttcg ccacacgttt aatcacctgg tggcgcgggt cagcgatggt
    31261 gtaaaccgga tgaccaaaac caatgaccac ttctttgttt tccacgcgct tgcggatatc
    31321 tgcttcggct tcgtccggcg tttcgtagcg ttgctggatc tccagcgaca cttcattcgc
    31381 cccgccgtgt tttggcccgc gcagtgcgcc aatcgcgcca ataatcgcgg aatacatatc
    31441 agagccggtg cccgcaatca cccgactggt aaaggtggag gcgttaaact cgtgttcggc
    31501 gtacagcacc agcgagatat gcatcgcctt ttcccagctt tgcgatggct tttcgccgtg
    31561 cagcagattc aggaagtgac cgccgatgga gtcgtcatcg gtttccggtt ggatgcgttc
    31621 gccgttgtgg ctgtagtgat accaataaag gagaatcgag ctaagcgacg ccagcagttt
    31681 gtcggcaata tcccgcgcgc cagagacggt atgcccctct ttttctggca gcgtgcagcc
    31741 gagcgcggaa acaccggtgc gcataacatc catcgggtgt gacgccgccg gtaaggcttc
    31801 cagcacggta cgcacgttag ccggtaaacc acgcagggct ttcagtttcg ttttgtaagc
    31861 ggcgagttcg tcacgggtcg gcagtttgcc gtggatcagc agatgagcca cttcttcaaa
    31921 ttcgcaatgt tccgccagat caagaatatc gtagccacgg taatgcaggt cattgccact
    31981 tttacccacg gtgcagagcg ccgtattgcc cgccggaacg ccggaaagtg ccaccgattt
    32041 tttcggttta atgacatggg tactgttttg caggatcgtt gtgtcgctca tattgtcctc
    32101 gtcatttgta gggtcgagat atttttcgtt gtgttttatt tcacctggct acgggcaaac
    32161 aggtcgtcga gcttctcttc gtactggtag tagttgatgc tttcgtacag ttcgttgcgg
    32221 gtctgcatgg tgtcgatgac gcttttctgc gtgccttcct ggcgcaggac gttgtagaca
    32281 tgttcagcgg cgcggttcat ggcgcggaac gctgaaagcg ggtacagcgc cattgcgaca
    32341 tgggcgctgc gtaattcgtc ggtggtaaac agcggcgtgg caccaaattc ggtaatgttg
    32401 gcgaggattg gcacctgaac cgcatcggca aactggcggt acatggcgag ttcggtaatc
    32461 gcctccggga acaacatctc ggcacccgct tcaacatagg cctgtgcacg ctcgatcgcc
    32521 gcatccagcc cctctaccgc cagcgcatcg gtgcgcgcca tgatcacaaa atcaggatcg
    32581 gttttcgcat ccaccgccgc gcggatccga tccaccatct cctctttcga gacgatcgct
    32641 ttgttcgggc gatgaccgca gcgtttcgca ccaacctgat cttcaatatg caatcctgcc
    32701 gcaccggctt taatcatcga tttcaccgta cgcgccacgt taaaggccga agaaccaaaa
    32761 ccgatatccg catccaccag cagcggcagc gaacaaacgt cggtgatacg gcgaatgtcg
    32821 gtcagcacat catcaagggt agaaataccg agatcgggca gccccagcga acctgccgcc
    32881 acgccaccgc cggagagata aattgcctga tatccggcac gctgcgccaa cagcgcatga
    32941 ttagcgttga tggtgccaac aatctgcaat ggattttctt tagacagtgc agcgcgaaac
    33001 gctttacctg gagagtgtag agacatagcc catcctttgt tatcaacttg ttacttgcgt
    33061 tgataaagac aaagcaaggg gtgtgccaca attaactgaa taaaattaaa tgtttaattt
    33121 caatttgtta taaataaagt catgaactaa tccgcaaata tgcgtttcag ttaacgtttc
    33181 aggcaatgtt tcaccgcgtt tcattgcaac aattatgaaa caagactaaa cccaatattc
    33241 ggtttcttaa ctttgcggtg cgctatggca catccaccac ggcttaatga cgacaaaccg
    33301 gttatctgga cggtatctgt aacgcgcctg ttcgagctgt ttcgcgatat cagcctcgag
    33361 tttgatcacc tggcgaacat tacccctatc cagcttggct ttgaaaaagc agtgacctat
    33421 atccgcaaga aactggcaaa cgaacgctgc gacgccgtca tcgccgctgg ctctaacggc
    33481 gcgtacctga aaagccgcct gtcagtgcca gttattttga ttaaaccgag cggctacgat
    33541 gtgttacagg cactggcaaa agccggaaag ctcacctctt ctatcggcgt tgtcacttat
    33601 caggaaacta ttccggcact ggtggcgttt caaaaaacct ttaatttgcg cctcgatcaa
    33661 cgtagctaca ttaccgaaga agacgcccgc gggcagatta acgagctcaa agctaacggc
    33721 accgaagcgg tggtcggtgc ggggctgatt accgatctgg cagaagaagc cggaatgacc
    33781 ggaattttta tctattccgc cgtcaccgtg cgccaggcgt tcagcgatgc gctggatatg
    33841 acgcgcatgt cgttacgcca taacactcac gatgccaccc gcaacgccct gagaactcgt
    33901 tacgtgctgg gcgatatgct cggtcaatca ccacagatgg aacaagtacg gcaaactatt
    33961 ttgctgtatg cccgctccag tgcggcggtg ttgattgaag gggaaacggg gacaggcaaa
    34021 gagctggcgg cccaggcgat tcatcgggaa tattttgccc gccacgatgc gcgacagggc
    34081 aaaaagtcgc atccgtttgt tgccgtcaac tgcggggcga ttgccgaatc gctgctggaa
    34141 gcggagctgt ttggctatga ggaaggggcg tttaccggct cgcgacgcgg aggtcgcgcc
    34201 gggctgttcg aaattgccca cggcggtacg ctgtttctgg atgagattgg cgaaatgccg
    34261 ctacctttgc agactcgcct gttacgggtg ctggaagaaa aagaggttac tcgcgtcggc
    34321 gggcatcagc ctgttccggt ggatgtgcgg gtcattagcg ccactcactg caatctggaa
    34381 gaagatatgc ggcaagggca gtttcgccgt gatctgtttt atcggctgag tattttgcgt
    34441 ctgcaattgc caccactgcg cgagcgggtg gcggatattc ttccgctggc ggaaagcttt
    34501 ttgaaagtgt ctctggcggc gctctccgcc ccgttttctg ccgcattacg ccagggatta
    34561 caggcaagcg aaaccgtgct ggtgcactac gactggccag gcaatattcg tgaactgcgc
    34621 aatatgatgg aacgactggc gctgttttta agtgtggaac cgacgccgga tttaacgccg
    34681 cagtttacgc aactgctact gccggagctg gcgcgcgagt cggcgaaaat tcccgctcca
    34741 cgcttactga caccacaaca ggcactggag aaatttaaag gcgataaaac agcagcggcg
    34801 aattatttag gcataagccg gacgacgttc tggcggcggc tgaaaaactg aactgccgaa
    34861 tgcagcgcga acgctttatc cggcataaat ttcaatagat gatgatcacc gataaattta
    34921 cgcatcgggg atgtttacat aggtttcaga attacttctt cttataaata tttgccgtgc
    34981 cgtggatctt attgtcagtt tgaccagagg tcagtaccaa cacatcagcc cctttttcat
    35041 ccgctttttt gatcaaatcc tctttcgcat ctgcggtcga catttcatta ctggttgaaa
    35101 tatcacctat tttttcatac tgcgattcaa ctttttcaaa ttccgctttg gtcatcaatt
    35161 cagcggcata gacattggta acggctaacg ctaacgcacc gactaacatt ttagagatta
    35221 ttttcatatt acggctccag agttgttatc acactatcac ccacccacat tattgggttg
    35281 attaataatt agttcagtca gggcataaac gccagtaacc ggaaataaga cactttgtga
    35341 tctctctcgc acccgattat ttttggtaga aatatgatga agttacttca cttatttatg
    35401 gatgaaatca ctatggatcc tttgcacgcc gtttacctga ccgtaggact gttcgtgatt
    35461 acttttttta atccgggagc caatctcttt gtggtagtac aaaccagcct ggcttccggt
    35521 cgacgcgcag gggtgctgac cggactgggc gtggcgctgg gcgatgcatt ttattccggg
    35581 ttgggtttgt ttggtcttgc aacgctaatt acgcagtgtg aggagatttt ttcgcttatc
    35641 agaatcgtcg gcggcgctta tctcttatgg tttgcgtggt gcagcatgcg ccgccagtca
    35701 acaccgcaaa tgagcacact acaacaaccg attagcgcct cctggtatgt cttttttcgc
    35761 cgcggattaa ttaccgatct ctctaacccg caaaccgttt tattttttat cagtattttc
    35821 tcagtaacat taaatgccga aacaccaaca tgggcacgtt taatggcctg ggcggggatt
    35881 gtgctcgcat cagttatctg gcgagtcttt cttagtcagg cgttttcttt gcccgctgtg
    35941 cgtcgtgctt atgggcgtat gcaacgcgtt gccagtcggg ttattggtgc aattattggt
    36001 gtattcgcgc tacgcctgat ttacgaaggg gtgacgcagc ggtgagcaga taatttactc
    36061 tctacgtaat caacctgatt tgccgaatcc cggccatcgg cgatgatagc ccccgtaatg
    36121 cgtggtcctt atttccgata agtttacttg agtctttagc ttcccatttc agcccactct
    36181 cccctgtgtg ggcttttttc attggtctgc cccgtcctga atagccaaac aatggcaatg
    36241 ttccactttg cagggatgat aataagggga tctgattcag aagagttttg aaaagttgga
    36301 ccgccatcga ggcctcgaac cccgtaccat acaacaatcc tggttgtcgt ctgctcttcc
    36361 tgatgagcta atggcggttt ttgatatgag ccttacagca tattttctgt caggcttgag
    36421 tgcgatgcta aggtacagaa aagaccccac acaaacatga tggaattatg atttttttca
    36481 tatgaatgtc aacccacaca ttcacaaaac atctgcatga gatcattttt tatacaccgc
    36541 atggctgcga aaccactact ggtgcttttt taagcgaagt gtcgccgatc acaacaaaat
    36601 atcggcgaaa aaagagatgg aatgccagtt ggcatacaga tatacagatg aaatgattac
    36661 ctgcattgcg attcactctc aggtggaata acaacttgca ggtggaaccc tttctgaact
    36721 aaagagtgta gcggatttta acgtgcttat ttacaggata atatctgtcg cctaattcct
    36781 ggactctatt agatatccat tgatttaatc gcgcatcatc tctgtatgtt tttacgtcca
    36841 gaacagagag caataacatc accagtgatt caggaaaaac agggtagata ttttttaagt
    36901 gtaataataa tccatgcgca agcgcctgct taatgggcgc gtctgtctcc ggaatagacg
    36961 acatttcgcg aagccatttt tccacatgca tacactcttt ccctttaggg aaaattctca
    37021 gcaaaggcat atggtgttca agaagaatac atttgcaatc tgttcgcact gaatcgataa
    37081 actgcactaa tttcttaagc ccggtaaagc gtccagcctg cacgtttgct ttaatatcaa
    37141 gaaacgcatc atcctggtca tatacccaaa ctgtcccttt ttgattttca agtatgcgaa
    37201 aaacaaactt aaccgggtga tcagggtaat ccatccagtc ataaaatcca gggaaatcat
    37261 taaactgcac atttatggtg agtgcgttat cgtctgacca ctcacagtct cgtacggcag
    37321 ccatgagatg aatgagtttg tcgtcatcga ctaaatcatc acgacaatca taaggataat
    37381 cataatattg tttaatcaaa accttgaata tatcgacaca ggaaaccagg gagcttttga
    37441 tatcctctgg taactgattg tttttaaccg tattaagtat gctttgttgc gtatatggca
    37501 tcaaattatt gtgcggagct tttaatgata tcatcgcgat ctctcttaaa taaaaataaa
    37561 cgtcgcagat aataaagctc tgattaaacc aggcactatc agaaatcgct cataactaac
    37621 gtgtgaagta ttgtgtactg gagggagtta atttaataat ttgaatttac gttataggtt
    37681 ttactgctac tttgatatta tttcgctctc cttaactaaa aagttaagtg gagagcgaag
    37741 ttaatatgtt aaaccacagc gtatttatta attttttcag tctgttagtg tgcgattatc
    37801 gataacaaaa cgatatttca catcaccgcg cagcattcgc tcataggctt cattaatttg
    37861 atcggcccga atcatctcta tatcagccac aatgccatgt tcggcgcaaa aatcgagcat
    37921 ctcctgagtt tctggaatgc cgccaatcat agaaccggct atcgcacggc gtttcatgat
    37981 caggttgaaa acttccggcg atttatgcgg tgtcgcaggc gcaccaacca gcgtcatggt
    38041 gccatcacgc ttcagcaagg tggtaaaatc gtcgagatta tgtggcgcag ctactgtatt
    38101 caaaatgaaa tcgaaactct tcagatgagc cgccatctca tcggcattgc gtgagttaac
    38161 aacttcatcg gcccccaggg cttttgccgc ttcgcgtttt gcctcagaag tggtaaatgc
    38221 caccacatgt gcccccatcg cgtgggccag cttaatcccc atatgtccca gaccgccgat
    38281 gccgaccacg cccacttttt tacccggccc ggcctgccag tgacgtagcg gcgaatacgt
    38341 ggtgatccct gcacacaaca aaggagccac cgccgccagc tgctcttgcg ggtgacgaat
    38401 acgcagaaca tatcgctcat gaacgacgat ctgttgtgag tagccgccca gagtatggcc
    38461 cggttcgtcc ggcgtcggcg agttataggt gccggtcatg tgatcacagt agttttccaa
    38521 cccgtcttca cactcttcgc aatgtttaca actgtcgaca atgcagccga caccgaccag
    38581 atcgcccggc gcatattttt ctacctgatc accaacggct accacacgcc ccacaatttc
    38641 atgacccggc acgcaggggt aaaccgtccc cgcccactcg gaacggacct ggtggagatc
    38701 ggaatggcaa acgccacagt aagcgatttc gattttgaca tcattcggtc ccggttcacg
    38761 ccgggtgata tccatcggtt caagtggttg tttagcggaa tatgcaccaa cagctttgat
    38821 cttcattgtg tttactcctg attagctatg tgtatttggc gacgcctgat atgtcatcgc
    38881 ggcaaaacgc gtccattgaa tatagccaat atttaaacca cccgatccag ccccaacatg
    38941 ccgcgagcct gaatgaaaca gagcagcaac gttccccata aggccatcgt caggtagggg
    39001 cttataccga gaatattgaa gccgctttca ataatttgca gaagaaacag cgccagcacc
    39061 ataccaatta tccgcccgct gccgccgtcc ggattaaccc cgccgagtac tgccgcgagg
    39121 attgacacca gcaagtacga ttcgccataa gaggccttag ccgagttgag tttcgacatc
    39181 atcaaaaatg ccgctaccgc acaaagcaag gcggaaatga cataaaccca aatcagcacc
    39241 cgacgagtgt taatgccgga gtaaagtgtt gctcgttcgt tagacccaat caggtaaatg
    39301 gctttaccga gcggcgtttt ctccagcaat atccatagcc ccagtgccac tgcggcaaac
    39361 aaccacatcg gtagcggaat accgaaccat tgcgcatgat tcaaccacag cacccatgag
    39421 ggataattgg caatggcgct accacctgtc accagaatat ttatcccttt gagcaacgtc
    39481 atcatcccaa gcgttgcaag aattggcgac actcgaatac cagcaatcaa tacgccgtta
    39541 cataaaccga ttatcaccgc cgcgccagcg cccgccagta acgtagcgac aaccgtagca
    39601 atacccggtg gatactgagt cgccacccac gccatcacca gtgaacaggc gttagcagtg
    39661 gctataattg acaagttaat gccaccgcaa agcatggtca ccgccatagc caacgccaga
    39721 atgcccagca cgggcatttg cgaagcgacg gactggaaat tacttatcga ccagaaaatc
    39781 tccggcatag taatgctgaa cgcggcaaca gttagcacca gcaggccgat caagtaaaac
    39841 tcaacgttat tacgccacga ttttttcata gcagactccg gttctgatgc tgactccacg
    39901 ccgtggcact aatgctggca acgatgatga tgccggtgat caatgtttgc cagtaagacg
    39961 agactcccaa taaatttagc ccgttttgca tcactgccaa caacaccacg ccgagcaaag
    40021 taccggttaa cgtgccgcgc ccgccgagca aactagtgcc gccaagcacc accgcagcca
    40081 gtactgtcag ctcataaccc agaagagaat cgggggcgac agtcatcacc gtccacgact
    40141 gcactacacc cgcagcgcca gacatcaatc ccatatagcc atagacgaaa agttgcagtt
    40201 tcagcacgct aaagccgatg cgactggctg attcgcggtt cccgccaagg gcataaattt
    40261 tgcgccctac actggtgaaa ttcatgataa acgctgttag cagcaccacc gtaatcatgg
    40321 cgatcagcgg cagaccaagg ccatagtcat agccatcagc atcggtgtac ttgaacaaca
    40381 taaccccctg ctcaaaccac ggcggaaagt cgtacagcca cacaccttta ctcaaccata
    40441 acagcaggcc atagaaaata ttcagcgtcg agatagtgat gatgatagaa ggcacccgta
    40501 ggcgattaac cagtaaggcg ttaattaagc caagtaaaat gccgatgcag cccgccagtg
    40561 caaacgcgac agcgaagttg ccaccaattt tttgcagcaa cagcaccatg ccgtattgcg
    40621 caatgatggt cattgctgga aacgaaatat caattccacc ggaaatcaac accacaaaca
    40681 aaccacaggc gagaatagtc aacatggcat agttattggc gagatcgtac aaattaccga
    40741 atgtcagaaa ctcgtcgctg cgccaggcaa ggccaacaaa cagcaccaca atcagtaatg
    40801 ccagccagaa ttcatggcgt tttttcagtt cagccattta ccacctccgc aatcctggcc
    40861 tgagaactgc tgtcaggcag gaagctatgg gtgatctgac ctttttgcat taccaaaatc
    40921 cggtggcttt gataccacgc ttcttcaatt tcatcgcaga tcatcaatac cgcaatgcca
    40981 tgagccgcca gatcgctgat aatgccgtat attcctgctt tgttggcgat atcaacaccg
    41041 actgtcgggc tatcgagaat caacagtcgt ggcccaatcg ccagccattt ggcgatcgat
    41101 actcgctgcg cgttaccacc agaaagcgta ttcaccggaa gatgaggatc agagactttg
    41161 atggtcaatg ccttgattag cctgtccacc aggtcacgaa ctttagtttc actcagaaag
    41221 ccgaagcgat cttttacctt gtgaaaaaca gtgctaatga tgttgtcttc aatagattgc
    41281 gccatcacca aaccgcgcga catgcgatct tccgaaacat aaccaatacc gtggcggata
    41341 gcgtcctggt tactatgcag tgtaaccggc tggccgttga tgaggatctc gccagcatcc
    41401 ggtcgggtca taccaaacag actcagacat aattccgtgc gccccgcacc aagcaggcca
    41461 acaatcgata ccacttcgcc cgcttccacc cgcaagttaa tatttagata ttccccatga
    41521 cgacttaaat tacgcacctc cagcaccggg gtgctggatt ttccctgcca caactctcgc
    41581 acctgatatt caaactcctg gccggtcatc aggaaaccga gttgttttgt ggtcatctct
    41641 gcagccggaa aagtcccgac cagctcacca tctttcagca ctgaaatacg gtcggaaact
    41701 tccatcactt cttccagacg atgactgacg aagaccacgc agatcccgcg ttcacgcaat
    41761 tgatgcacca cctgtaataa cccttgcact tcctgatggg tcagcgaagc ggttggttca
    41821 tccatcacga tcaaccgtgc gtcctgcgcc agtgcacggc aaatagccac cagctgacag
    41881 cgggcaattg aaagctcaga aacgagtgta tccagtggca atgtgacgtt aatactggtc
    41941 attgccgcct gcgccacctc acgcagacga cggcggttaa caaacagacc gtggtgatag
    42001 tgattcacgg cgatattctc ccagacactc aggttaggaa ataaagagag gtcctgataa
    42061 atcacctgaa tcccctgcgc caccgaagcg gcgggtgtca gctttgacca gtttttccca
    42121 ccaagcgtaa tttccgctcc ctcatccggg cgataaacac cggacataat tttaatcaac
    42181 gtcgatttac cacagccgtt ttgcccggca aggcagtgca cttcacccga cataaagtcg
    42241 agagaaacat cgcgcagcgc tcgcgtggca tgaaacgttt tgttgatatg acgaagggaa
    42301 aggaaagttt ccataacatc ccctggcaat aagaatcggg gctttcgccc cgaagaaatg
    42361 gattaataaa gtgagtcgat attgtctttg ttgaccagta ataccttatg gaaacggata
    42421 attcgcttat ccatatctac gtcggcctta cccagattct gcatttctaa tccaggtttg
    42481 atctcttcac ccttcagcaa cgtgctggcg acggctgcga gtgcgtaacc tgcagaggcc
    42541 ggatcgtaag taatcccttc ggtgatatca ccacttttaa tcagtgatgc cgcctgtgaa
    42601 gggatcatca tgccatagac tgcgacttta tttttcgccc gtttctcttt caccgcacgt
    42661 cccgcgccaa tcggaccgtt tgaaccaaag gagacaaccg ctttcaagtc aggataggtt
    42721 ttcatcaggt ccagtgtggt acgacgtgag tcatccacac tctcggcaac cggcatgcgg
    42781 cgggtaactt catgcatatc cgggtaatgc tctttctggt atttcaccag caagtcagcc
    42841 cataagttat gctgcggcac ggtcaaacta cccacgtaaa tcacatagcc gcccttgccc
    42901 cccatgcgtt tcgccatatg ctcaacatat tcagcggcaa atttttcgtt atcaatgatt
    42961 tcaatatccc agttagcact tggctgaccg ggggattcgt tggtcagaac cacaattccg
    43021 gcatctcgcg cttttttgaa taccggttcc agcacgttgg catcgtttgg cacgatagta
    43081 attgcattaa ccttacgggc gattaaatcc tcaataattt taacttgttg cggagcatca
    43141 gtacttgaag gccccacctg tgaggcatta acaccaaagg ctttacccgc ctcaaccaca
    43201 ccttcgccca tgcgattaaa ccacggcata ccatcgactt tagaaatatt caccacgact
    43261 ttttccgctg cctggagcgg cgcagaaatt agcgcagcgc ctaataacag cgaagacacc
    43321 atattgataa cgaaacgttt attcatcatt tcagcctcat tgcagggtag tcaggaagtg
    43381 ttcgcccaaa ttagcacgct ggagagaata agaattttcg aatattttta tgcagaataa
    43441 acagccctat ttcaggcagc tattaaaaat atggttataa atatggacaa aacgggcagc
    43501 aacaataagt gtgacattca tcacgtaaaa aaatagcaaa ataaacaaaa ttagcaatat
    43561 tttatgtgtg tgagtttatt tcgcgtagat aaatagttat ctacacaacg tcaataaggg
    43621 catattcttc aaatataacg tccatttctg gtgaatagct tccttctgcc tggaaaaaac
    43681 gacggtgttc attacgccaa tattcaagac tgaggtcacc ttcaccttcc ttacgggcaa
    43741 gctccacggt gacatcagaa aagcgcagta aatgcaggcc aatcactctg ataacacata
    43801 caggctcacc acggctattt tctacaatct tatactcacc aatcatcgga aatgcgttgt
    43861 cttcaataca tcccgccagc gaaccacagg aagctgtttt aacaccagtc gcaatcaacg
    43921 ttgcgaggtg atcggccaac gctgctgaat cgccaaaagt acaaaaggaa gcatggggat
    43981 atttttgtga gaatttttct tttatggtca tggcaatatt tttgcgatta cggaaaaaca
    44041 aatcacatct taattactgt tggtaaccat cagcaagagt atcattggtt tttcgatact
    44101 gaatcttttt tatccgaagc aggtcacatt tatggcctga taaatacctt tgttaaaact
    44161 cacttttgtg tatttctctc aaataatccc ctggaaatta tgccgcgaga ttataaagtt
    44221 caggatcatt cattagcaaa ccattcccgc cccactccct ggggcttaaa tctcttacaa
    44281 ttcaccgttg caggcgtttc aaatgcgcat caacctgcca cactccccca caccagttgc
    44341 cctttatgga acgttgcggt tctcggcgag atacgcgcca ccgcctcggc ggaacaggag
    44401 gcgtccacca gcacaaagct ggcatcatcc tgcgcttttg gccatacacg ctcgcctttt
    44461 tcattcagcg gcaatacatc gccagtggct agaaacagcg aacgggagag gttctgttcg
    44521 ttaggacgaa tatagagctg cgcgtagaga ttggcttttt ccagcatgtc gcccagacca
    44581 taaggcgacc agtggtcgat aacgctgtca gtgccagtca tcacttttac gcctttgtcg
    44641 tgcaactgtt tgagcggcat atgcagcgtg ccaatcggca ccgtcgaggc gatagaaatt
    44701 tgctgcgccg ccatccgatt cgccagcgca tcgacctgtt gttcgttgag cgtagccagc
    44761 gcaaaggcgt ggctgatggt cagcttacct ttcagttgcg gcgttttctc taccgtttca
    44821 accatataat tgatggctgc cacgcccgac ggagtggttt cgtgcaggtg aatatcgaca
    44881 cctttgtcgt agtccagcgc aatctgaaac atggtgtcga gggatttttc catcgcgcca
    44941 tcgacactgg tcgggtccag cccgccgacg taatgcgcac ccgcctgcat cgcttcgcgc
    45001 attaagggtt ccgatttcga cagcagcaaa ccgtgctgcg ggaaggcgac gatttcacac
    45061 tcgaagcccg cctgacgtcg cgccagcacc gcctgcaaat tctgcagatt tttcaggccg
    45121 gaaaccggtt cgatattgca gtggctgcgg gcaatggtgg tgcctttcga ctgcaataga
    45181 tcgatcagtt tttctgcccg ttcctgcgtg tacggttgca gttccggcag cattttctgc
    45241 tcaagcttga tcatgtcctg gatagtggtg cctgccggac gattgagcga gcgccacggc
    45301 ccgccgtaga aggttttgtc gagatgaata tgcatgtcgc gagtggtggg tagcatcaac
    45361 ttaccgccag cgtcatagtg cggcagcgtg gcgtccggat gctgcttgtt ctcgcgcagg
    45421 gcgacaattt tgccgtcctg aatctccacg gtctggcgcg ccgtgcgggt ctgcaccgcc
    45481 acaccgtttt cgtagtcaaa accggtttcc agcagcacgt tatcgagata gtaatgcggg
    45541 tcagtgattt tcatggtgtt gttcgcttcg cagtttaggg tgccagctac cgccgcatgg
    45601 gcgagcggca cagaggtacc aaacagcgcg gcggcggtga ccatcttacc gctctggctc
    45661 agaaattcac ggcggctatt gctttctttc atcctacgtc cttacgtttt aataatatgg
    45721 gtgcccgttt caccgcgcag cgccgcaggc aggcattccg gcgtggtgat aatcacttct
    45781 ttgccgccct gctccaggaa cgtcaggctg gcgatgattt ttggcaacat gctgccgggc
    45841 ggaaaatgcc cttcctgcat atagcgagtc atggtggcaa tatccacccg atccagcgcc
    45901 tgctgctgcg gtttaccaaa gtggatacag actttttcga cgccggtggt gatcaccaga
    45961 atgtcggcgt gaatttcacg ggccagcagc gcggtagaga gatctttgtc aataaccgcg
    46021 tccacgcttt ggtaatcccc cgcttcagta cgcactaccg gaattccacc gccgcccgcg
    46081 ccaattacga caaaaccttg ttggatcagc gccttaatgg cgggtgcttc gacaatacgt
    46141 ttcggttccg gcgaggcgac cacgcggcga tagccccgtc cggcatcttc aacaaaacac
    46201 cagtcagggt ttgccttttg tagtttgtca cgctggcttt cactgaagaa tgcaccaatg
    46261 ggcttggtgg gatgggcaaa acctggatcg tttttatcca cttccacctg agttaccacg
    46321 gtgacggctt ttttctcacc gtgacgcgcc agccggttat tcagcgcctg ttggatcaga
    46381 tagccgatgc cgccctgcgt atctgccaca cagttcgcca gcggcgttaa gggcagccct
    46441 tcgcgctcgt gggcaatctc cgcacggcgt aaatccagcc cgacctgcgg cccgttaccg
    46501 tgggtcagca caatgtcgta atcggaagcc agcatttcca gcaccgtatc ggccacggct
    46561 ttcaccgcct ccgcctgatg ctcaatcgac tggctggcgt tatctttgat aatgctgttg
    46621 ccaccaatgg caacgaccac aagctctttc atgttgttat gtccgctaca gagggtgatt
    46681 aggttatgac caacgccaga ctgcctgatg cgctacgctt atcaggccta caattctcgg
    46741 gccatccgta ggccggataa ggcgttcaca ccgcatccgg cataaacatg taagtcagac
    46801 gccgtaacgt tcgcaccagc cgagaatggc tttctcgaag caggcgaatg gtggatgcac
    46861 aatgcccgcg ccaatcatgc cgacgcccgc atctttatgg gcgatggcgg tgttgatcac
    46921 cggcagaatg ccgctgctac ccacgcgggt gatgtcgatt gccgacggca cgcccataaa
    46981 accgagcagc ggaatggtga cgttggggtt ttcgccaagg gtgatttcgc gcatctgacg
    47041 ggagaaatcg atagcctctt ccaccgtgcc gcccaccagt gcgacgattg caggcgcggt
    47101 cgccatggca aacccgccaa taccgtaggt ttctgtgatg gcgctgtcgc cgatatccag
    47161 ccccgaatct tccggcttat aaccagcaaa catcgggccg atgacctgct gcgctgggcc
    47221 ggtaaaccac tgccccggca ggccgctgac ccgcaggccg aactcgacgc cgttacgcgc
    47281 catggtggtg accacggtgc tgtattcgat gccgtgcgcc gcatccatcg ccgctttaca
    47341 catcgccatc cacgtcgggc cggagaagta gtcgctgctg gcgacaaact cgaacacttc
    47401 gcgctgttgc tcgacggaat agcctgcctg aatgatcccc ggcgtcagtg cctgaatcag
    47461 cagcgtcgtc ccggcgttat tgcggttatg gcactcatcg cccatatgca gcgcctgcgc
    47521 cagcattaag cgcagatcga tctcaccgat aattttcatc gcgtcgcgca gcattggccc
    47581 ctgcacatcg cgcatccagt tgaggcggtc gatcacgctc tgatcgttgg cacccatacg
    47641 caaaatcttc gccatctgct cgctcatatt ggtataagcg atgttgccgt aggttttgtt
    47701 tttcacgatg tgcataaaca tcgaggccga ggtaacgccc gccatcgatc ccacgcagtc
    47761 gtgttcgtgg cacggcgaga aggtgatctc cccggaggcc gccagttcag tcgcctcatc
    47821 gagatctttc gccagccctt cgaacaccaa cgccccggtg accgcgcctt tcatcgcgcc
    47881 gcacattttt tcccaggtga tcggcggccc ggcgtgaaga atggttttcg gcgtcatgcc
    47941 cggcaccacg ttaatcgcct gatcaaaacc aatcagcacc ggatgcgact ggataatgcg
    48001 ctccagcgcc tgctggttag cggcggcgat tttgtctgcc agcggcgaat cggcaatgtt
    48061 atccagcgcc tgcaccacct gcatattgcc ctgccccggc ggcgtccagt cgagttgagt
    48121 cacttctaca tgctgctttt tcaggtcatc gctaaacatg gcgataccga cgttaataac
    48181 gttcaacggt tggctaaaca gtgactggct catcaggctt cctccccttt gcagataaat
    48241 tcacgcgcca gcaatccggt attggtgctg ctgctcgcca gaatcactcc ggcatcaagc
    48301 agcatctggc tttgttgttc taatgatggc gtatcgagat cggtgcccag cacataggcg
    48361 agaatgatca actcgcgtcc ttcggcggcg gcgatcgctt tcgcttcttt gatcgcctcg
    48421 atggtggagc cgaccggatc ttcatgcgat ccaaatccga gcacaaaatc catcacgatc
    48481 accgccactt ctggatcgcg cgcctcttcg atcaagcgac tgatgcggtt ggtggggtca
    48541 atcattggat gcggcttgcc gttggtgaag tcgtcatcgc caaagtcgag gaaggtgtgt
    48601 ttgatgctac ggttgatatc ttgcaggcgg aattccggat cgggctgaat gttgctgtag
    48661 acatcgccgt gtttttccat caccgcgaac atggtttcgt cacacagcgt gccgccacag
    48721 aacaggccac gaatgtactt ctgctgcggt tgcaggcgag cacgtacgtc agcaatcaac
    48781 ggctggttca gcgtatgcag gtcgagatgt tcctgtttca cgccggagag catcaccgct
    48841 ttcaatgccg cctctttgct gccgcgggca aactgtagcc cctgctcatc cactggcgtt
    48901 tcgccacgac cgaggaaaca gaccaccacc ggcttgcggc aggcgcgcgc gcgttccagc
    48961 actttgcggg ccaccgcagg cgcgggcggt ttggagataa gcacaatgat ttcagtttgc
    49021 ggatcgtttt ccagcatgcc gatggcgtcg agcatcatca ggccgccgat tttctcgctc
    49081 aggtcgcgcc cgccggtgcc aatcagttgc gaaacgccgc cgccaaattc atgaatgcgg
    49141 acgctcagct cctggctgcc ggtgccggat gcgccaacaa taccgatgtt gccgcgacgc
    49201 acggcgttac caaaacagag cgccgcgccg ttaataatcg ccgtgccaca gtctggcccc
    49261 atcatcagca gccctttttc gtgggccagt tgcttgagcg ccaattcatc ttcaactgag
    49321 acgttatcgg aaaacagcat cacgttgaga tcgttttgca gcgcctgacg cgcttcgcga
    49381 gcggcaaaca gaccgttgac cgaaatcacc gccaggttac tttccgggat atgctttttg
    49441 gcgctggcaa tagtggcgta acgcgcctca tgcgagccgc tttgcgcttt ggtgttgaac
    49501 agttcttcaa tctccaccag caactgttcg ttgtccgcgc ccgctttgcc gttgatgaca
    49561 atcatcaggt cgccgttttt cgcctgttcc agctccggcg tcagcagtcc taaatttttc
    49621 agtacacctt tgttcatttc ggtcgccatc gccacaaatg cctgctcgac gccgtcgagt
    49681 ttatttgcac gcgtggagat agacatcagc gagacagaat caaaataggt attcggttta
    49741 atgactattt taactgacat aattccctcc gtaggtttgg ctcagcgcac aaccatcggc
    49801 catgccatac agcatgtcgc agccggaact ggagccaata tgtttaattt tttctattgc
    49861 ctgagttgcg ttcccaggga tgtcataaat aatgttgtga ataaaacgat gaatattttc
    49921 ccggcaacgt tgttgtaatg cggcttccag cgttatagca cttaataatg tggtgttatt
    49981 tctgccgcgc tgcataccga gataaaattc ctctttgtat ttttccgacg gatgcccggg
    50041 aataaataaa ataagcgcca gacctgttaa ataatcgtcg gctgatggcg taaggccaat
    50101 ccctaaccca atcatactgg cgaccgctgc ggcgatattt tgtttttcgt ttactgcctg
    50161 aataactgcc cgtcgtttaa tatgtagttc tttatttaac gcctgataaa acggattatc
    50221 gccgcgataa ttaaacaggg tttcattttc ccccaggtgc tgatgaataa tgtcgtgcca
    50281 ctgtagccag gcgataaggt gaaaattcac agcggtcatt tgcaaggttg gggacagcca
    50341 acgccgacaa cgactcatct ctatatgaag atgttgacca accgtaatac cttgatcgtc
    50401 aaactgaacc ttatcaccat gccggaacag atcatcaaag tgagtgagtg ccaaccgaca
    50461 actgtttggc gcattgtcgt actcttcaca aagcagcgtc agcaactgct ggcgagccgg
    50521 gagtgcgata tttaccgctc tggcaaagac ctgttcaacc tgtccttgcc cccgctgcgc
    50581 cagaaaatcc gcatccgcag ttagcgccca cacacaccgt ctttctggtg tgttgatagc
    50641 aagcctcccg gtttatgcac ctgcggcaat cagtaactgc gcaatctctt caaagccccg
    50701 ttcccgcgcc agttccagtg gcgttttgcc atatttatcg gtcagatgcg ggctggcacc
    50761 gtgttccagc aataactgca caatcgcctg ctgtttaata ccaccatcat taagcacaat
    50821 cgcttccagt agcggcgtcc agcctacatg gttggtctgg ttaacgttaa tctccgtatg
    50881 cgccaaaagc tcttttacaa tacttaaatg gcctttttca caggcaggcg tcaggccgac
    50941 accgccaaaa cgggttacgc aattaagatc gggtttagcc ggtaaaataa ttcgtagtag
    51001 cgttaaatca tcgttcagac agctaattaa aaaaggattt aaacaggtat gatcttgctt
    51061 attaatatcc gctccggcat caattaatgc ctgaacgcaa gcatattgct gatataaact
    51121 tgccagcgta attgccgttt tcccctgacg atcgcaggta ttaatatcga cacccagcgc
    51181 aaggcaggtt tttactttat cgatatcacc ctgttgtgca gccaataaat agtcggcagg
    51241 tagatttttt atactcattc acattcccct ggtttttgtg gctgttttcg atgaattgag
    51301 aatagcaacg ataatctgat aaaagaaccc gcttaaattt aattcgctgt atttcgcctt
    51361 tttgttgaat gatgttcaac aatcttttcg cagtgtgcgg tagtgcaata tttgtttctt
    51421 tgacccgata ccctttcact caatcaggtt ttatttctct atagtctgca catgtaagag
    51481 tagctttcgt ccatgtctaa ccagagggga acaagatgaa cggactcacg gcaacgggtg
    51541 tcaccgtcgg tatttgtgca gggctttggc agttagtgtc ttcacatgtc gggctctctc
    51601 aaggatggga attattagga accattggct tcgtcgcctt ttgcagtttt tatgccgcag
    51661 gcggcggtaa atcggggttt atcagaagtc tggcggttaa ttattccggg atggtgtggg
    51721 cgtttttcgc cgcgctgact gccggttggc tggcctcggt gagtgggatt tctgggttct
    51781 gggcgagcgt gattactacc gtgcctttct cagcggtagt ggtctggcaa ggacgtttct
    51841 ggttactgtc attcattccc ggtggctttc tcggcatgac gctgtttttt gcctgcggga
    51901 tgaactggac ggtgacgtta ctgggttttc tggcgggtaa ctgcgtgggg ataatttcag
    51961 aatatggcgg acaaaaatta agcgaagcga cgactaaacg cgacgggtat taaaagttat
    52021 tcaaaaatga ttatctatct ttaatcgata cctctacagt catggctgga gaaattcata
    52081 ttagataatg gcgggaatgg tttaatgaac tcagttttta ccgaagagaa tctgttggca
    52141 tttactaccg ctgcgcgttt tggcagtttc agtaaagccg cagaagagtt gggtttaacc
    52201 acttccgcca ttagctacac catcaagcga atggaaacag ggctggatgt ggtgctgttc
    52261 actcgcagta cccgcagcat tgagttaacg gagtccgggc gttatttttt ccgcaaagct
    52321 accgacctgc tgaatgattt ttatgccatc aaacgtagca tagatactat ttcgcagggc
    52381 atagaagcgc gtgtgcgcat ttgtattaat cagctgcttt atacgcccaa acacaccgcg
    52441 cgcttattac aggtgctgaa aaagcagttt cctacctgcc agattaccgt caccaccgaa
    52501 gtgtataacg gcgtctggga tgcgattatt aataatcagg ccaatatcgc catcggcgca
    52561 cccgatacac tactggacgg cggcggtatt gattacactg aaattggcgc gattcgctgg
    52621 gcttttgcta tcgccccgga tcacccgtta gcctttgtgc cggaacccat cgccgaaagc
    52681 caactgcgtc tctaccctaa tattatggtg gaggacaccg cgcatacgat taataaaaaa
    52741 gtgggctggt tgctgcacgg gcaggagtca attctggtgc cagatttcaa caccaaatgt
    52801 cagtgccaga ttttaggtga aggaattggt tttttgccgg attacatggt ccgtgaggcg
    52861 atgacgcaat ccctgctggt tacccgacaa atccataatc cgcgccagga ttcgcgcatg
    52921 ttactggcga ctcagcattc tgctacgggt caggtgacgc agtggattaa aaaacaattt
    52981 gcgccaaatg gcatattaac ggggatctat caggatttac tgcatcggga gaattaatcg
    53041 ctgccaacct cccgatgcgc tgtgaatagc gatttacatc aaccacctgc tttcattacc
    53101 cattcagaga taaatttatt acccggtact ggcggagagt acaaatagcc ctgcaaaaag
    53161 tgaacgcctt taccgatcat taaatccgcc tgctcctggg tttctacccc ttccgccacg
    53221 atactcagac caggcttacg cgctagttcg acaatattgt ccacaatatg accggagatt
    53281 tcgtcgacac tcgccatttg cacaaatgac ttatcgatct taataaaatc gaccgggaac
    53341 gcctgcaagt aacgataggt cgcataaccc gtaccaaagt catccagcgc aaaggtaatg
    53401 ttgtgctgat gaaggctgtc aaatatcgct ctggcttctg gcgttaccgg aatagggtta
    53461 cgttccgtta gctcgagaac cagcttgatt ttatcgttac ctaatttctt aaccaggttc
    53521 agacactctt tttcaaatcc cgctgccaaa aaacaacccg ccgagacgtt gatgccaata
    53581 tggaaattgt ccggcagcaa atgttttacc ggcatcagaa tatccgcagt ctgtttcatc
    53641 agttggcggg tcattatgac aataagaccg gatgactccg ccagaggaat aaactgatcc
    53701 ggtgggataa ttcccgtttg tggatgttcc cagcggacaa gtacctcaca gcccgtcagt
    53761 accccagtct gcgcgcagaa aaccggttgg atccacggtt tgaattcgtg gttttccaac
    53821 gccagactga tggcttcagg cgtgacgata tggtgacaac ggttatcagg gatataaaaa
    53881 ttatttttat ttcccgtgcc actgatgact tgcggatgat attgaaaaaa gatatcccgc
    53941 cagaaggtaa tatcactctg gatgcctagt ttttcataca gctgcttctt ttgatgtgaa
    54001 attgtctttg cactgcgatt tctgtactgt gagatttctg taaccgacat tccctccatt
    54061 agcagcttga gtaaaagccg ttctttttcg gacacgatac caggcaacgt caaattcacc
    54121 gttgccccga actcttccaa aaaaacactc aaatcatgcg aagccatcga acacaccttg
    54181 attaaatctt gtgcgccagc taaatctgcg agtcttcact ctgactgata aagctggctg
    54241 caagaaaatg acacagagag ccgaattatc tcccgttcgg ctctctctcc ttttactcat
    54301 tattcagatc agaattgcca cttaatgcca accattgcag aggtgtcatt atatccccta
    54361 tcggcaacct gaacgcccac attcccccag acattcaggt tggcatttag ctgtccttcc
    54421 accccggttt ttatctcagc aatatttcgg gctccatcct gagtgacgga cacgccatcc
    54481 atactggtgc tgaaatcctt actgttatgc agccagttca cttctacaaa cggctggaac
    54541 tcgcgggatt taccgtcatc cattttatgg tggctcttca gccaggtttt tacgccaagt
    54601 cgggtttgaa cattgccatc accgttgcta tgaacgaggg ttccgttgct ttcgcggtgc
    54661 ttatcggctt tgactcccat ccaggtaacc tgtgcttgcg gctgaacata ccattcatta
    54721 cgcgttccct ggctgccatt aaattcagct aatttgtgtt tgtatccagc ttccagtgaa
    54781 gcggtaaatc cttttgattt ataggattca ctttgtaagt catccccttt cactgtgtta
    54841 tcaaaccagc tgtactgcgc ccaactgtcg agatacgcgc cattacgcga ctcgtcatcg
    54901 gcataccagg tggcatagag gccggtgcta tatccgttca cactcgcttt tgcacgataa
    54961 ccggttcgcg aggaaatggt tttgctgtcg ctgttgccat atcccgccat gaccccaaca
    55021 tgccagctgt cgctgccgtt ttggctccac tgcgcgacat cgcctcccag ttgcagaaca
    55081 tagcgattgc tttgggtttt cagctggccg ctgccatcac gccatttatt atgaccacct
    55141 tcatggcgca tccacatagt ggtttgtttc tgctcacccg tcaccatgtc ggtatagtac
    55201 gtattaccca gacgctcatg cagacgcgtg gtgaacattg tattcgctgc tgcaaggttc
    55261 gcaatgtagc tacccgcctc cggtcgcagg tcattatcca cattcagatc cggacccggc
    55321 gtaggtgtcg ggttagggtt cggctctgga ttcgggtttg gctccggatt cggcatcggg
    55381 tctggctccg gctgcagttc aggagaatca ctgccgctgg tcagatacca gttaccacta
    55441 tttgcccctt gtccacgcgc gagagtgtag tcatacgccc ccgcaacgat acgaccagcc
    55501 tgaacaaatt cgccctcaga cttaccgtct acatggataa gttcgatacc gttaagggtt
    55561 ttcgcacctg taccgccagc gttattcacc gtaacggcag tcgtgccgct agtatcgccc
    55621 tcgacaacca atttatcggt tactgagtca tcgccattca gtaccgtatt cattactagt
    55681 tgaccgttat taccgtggta gttccccttc accactaaca cattgccagc ttgctgtcct
    55741 gacattccgg tcatcagcgt accgttgttg gtcagattgc cgcccagggt gtaattcaca
    55801 gacgcagccg ctcttttttc agcgctatta ttcaacgcca gggtaccgtt gttctcaacg
    55861 tttgcgctac ccaatccctg cgcatgactt accaggacat tagccgaatt agcaatgttc
    55921 acattcgccg tcaggttgct gttatcgccc gcgatgtcca gcgtgccgcc ctgaatattc
    55981 agctctccgc tccccgttaa cgaaccggtt gaagtgcccc cgttggtaac cgtcagactg
    56041 ccgccattta agctcagcag tgaatcggcg ctgccattga gcgtgcccac ggtctggctg
    56101 tgaccattca tatccagttc agtctctgcc gccagacgca gttcatgggt attacccaac
    56161 acgttatcgt tattcaacaa aagcgtccca ctgcgcagat cggtaacacc ggtatagtcg
    56221 ttgtctttgt tagaaagcga tacggtctga cccttctggc tgctgaatgc caggtcacca
    56281 ctgccggtaa tctttgcgct gagatcggcg gcattctcgc ttttaccgtt agagctcagc
    56341 accagagcat cgctgtcggt agcgtgtaaa tcaagctggg tcaggccgta accgatatac
    56401 agaccgtcat tgtttacccc actcgtcaga cgatagtcgt aagtgccgac ggccgctttt
    56461 tgcccattct gctgaatatc acgttgggca ctgctggaga ggatttgccc atttttatcc
    56521 tgcaattgca gattgcccgc atcgcccaga acttccgcac cttgcgcatc aaccaactta
    56581 atggtgctgt taccatcatc gacttcagtg agcgataacg cagagtcaat atcacgagat
    56641 actgagctta caacgtcact gtcagaaacc tgaatcacac cctcgccgcg caaatccagc
    56701 gttttactaa cgttgaccgt cccttctgtc atctgtgcac ctgctgtcag ggcaccaaaa
    56761 tcaacggttc cgccggagaa tcccaaagtg ctaatggagt gagcgccctt acccactatt
    56821 acctggcttc cctgacccgc agtgatcccg cctctctgca gaaactcagc attcttttcc
    56881 aggttaaatg tcgtgttctg gaacgccagt gaaccctgga accagtctgt ataagcaaac
    56941 tcatcgaagg agaattcgtg attccccatg tttatttcaa caatacctct accttgaaga
    57001 tcgttatcaa atacccaatc tttattggca ttgatttgca gtttgccatc aacatccagg
    57061 tccgcacggc cgatgttctt gtcttcagaa ataaccgcga cagcaccaga agcgacatca
    57121 atatatccgc tgaatgcata gttattgccg gtcagtgcca ctgtcccact gtcgatactt
    57181 attgtaccat tgccggaaat cttattggca aaggtcatgc tatcgtcagc cttcaggtgt
    57241 aatttcccgc ggttgttgat ctctgctgta cctatatttt gggtgatccc taaagcgcca
    57301 ttttgctcaa ttttgacttt accactaaag gcagagttat caccggttaa gtaagtcgtg
    57361 ccattgagcg cattgatata gcccaggccc tcgctgccac tgacaatatc cgcggaaata
    57421 gtcgcgtttt tatccgtgtg gtttaaggtg attttcttat cggatgtctt tgcccacaca
    57481 aactcaattt tttcggcatc aataatccct gcagccttcg cagcacttcc ttcctgtgca
    57541 cctaacgcta actcagagtt ggtgcttaaa ctaattttcg gtgcggatat tttacctcca
    57601 tctgatacga ctaatgttgt atcagaacgt tggtcgtaat aacccaggct aatatgttca
    57661 cgagcattaa cttcaccgcc gttattaact gacaggtact gattaccacc gtaactgccg
    57721 taaacgccca taaagaaggt tttagaattt acctggctgc cttcaccact gacatttccc
    57781 tgcaaatagg tcagacggct ggtgccctta acatggattt tgccgccgtt ggagacatta
    57841 aaatagccgc gatcgtgtct catgcctgag tttgactcat acaaatcatc agcatataac
    57901 tcgctgccag cattggagac attcaggaac cccgtacctt tcagcaaaaa gttaagtggg
    57961 ccattgatag ttacctttcc tccggtaata tccaacccac caccagaagc gagagtcaga
    58021 cgcggacggg aagtatctgt cgcattgatt accagattgg cattgtcagt caccgtaata
    58081 cggtaatcga tgctctggtt ctctttgcca aaatcaatag ttttatcctg gtttacgatg
    58141 acatcatcgg cgaataccag agatgaatat aaacttatcg cgctaattaa aacagcccgg
    58201 gacttttttc cttttactct gctccccagc tccgagcaga cgctccaaca gttacgggac
    58261 ctgttccact ttagccgata tattttattc atcacaatct ccagcaatac atccaaataa
    58321 aaccaagatt gcataaacag caacgcatta aatgccattc tggttactgt tcatcgcata
    58381 taaaaattca aaaattttgc atagcattgg caaccacata tctgagtatg tagttcagag
    58441 ggtaataaaa tagcaagtta ttacttaaaa cttattttaa gttaacactc ttaactttat
    58501 cattacattc ct
//
LOCUS       DAZGSP010000017        58151 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZGSP010000017 DAZGSP010000000
VERSION     DAZGSP010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.017"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            17..91
                     /gene="tnaC"
                     /locus_tag="ACHK74_001191"
     CDS             17..91
                     /gene="tnaC"
                     /locus_tag="ACHK74_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFP0220049.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            312..1727
                     /gene="tnaA"
                     /locus_tag="ACHK74_001192"
     CDS             312..1727
                     /gene="tnaA"
                     /locus_tag="ACHK74_001192"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFP0220050.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1818..3065
                     /gene="tnaB"
                     /locus_tag="ACHK74_001193"
     CDS             1818..3065
                     /gene="tnaB"
                     /locus_tag="ACHK74_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFP0220051.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3197..4372
                     /gene="mdtL"
                     /locus_tag="ACHK74_001194"
     CDS             3197..4372
                     /gene="mdtL"
                     /locus_tag="ACHK74_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFP0220052.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4347..5306
                     /gene="yidZ"
                     /locus_tag="ACHK74_001195"
     CDS             4347..5306
                     /gene="yidZ"
                     /locus_tag="ACHK74_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFP0220053.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWSLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5463..6212
                     /gene="yieE"
                     /locus_tag="ACHK74_001196"
     CDS             5463..6212
                     /gene="yieE"
                     /locus_tag="ACHK74_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFP0220054.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPLHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            6234..6800
                     /gene="yieF"
                     /locus_tag="ACHK74_001197"
                     /gene_synonym="chrR"
     CDS             6234..6800
                     /gene="yieF"
                     /locus_tag="ACHK74_001197"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFP0220055.1"
                     /translation="MSEKLKVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6854..8191)
                     /gene="adeP"
                     /locus_tag="ACHK74_001198"
     CDS             complement(6854..8191)
                     /gene="adeP"
                     /locus_tag="ACHK74_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFP0220056.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGNVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8358..9023
                     /gene="yieH"
                     /locus_tag="ACHK74_001199"
     CDS             8358..9023
                     /gene="yieH"
                     /locus_tag="ACHK74_001199"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFP0220057.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9090..9563
                     /gene="cbrB"
                     /locus_tag="ACHK74_001200"
     CDS             9090..9563
                     /gene="cbrB"
                     /locus_tag="ACHK74_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFP0220058.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9612..10199
                     /gene="cbrC"
                     /locus_tag="ACHK74_001201"
     CDS             9612..10199
                     /gene="cbrC"
                     /locus_tag="ACHK74_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFP0220059.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10261..10983)
                     /gene="yieK"
                     /locus_tag="ACHK74_001202"
     CDS             complement(10261..10983)
                     /gene="yieK"
                     /locus_tag="ACHK74_001202"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP0220060.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(10998..12167)
                     /gene="yieL"
                     /locus_tag="ACHK74_001203"
     CDS             complement(10998..12167)
                     /gene="yieL"
                     /locus_tag="ACHK74_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFP0220061.1"
                     /translation="MNIKIAALTLVIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPVLKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(12194..13810)
                     /gene="bglH"
                     /locus_tag="ACHK74_001204"
     CDS             complement(12194..13810)
                     /gene="bglH"
                     /locus_tag="ACHK74_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFP0220062.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDL
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13896..15290)
                     /gene="bglB"
                     /locus_tag="ACHK74_001205"
     CDS             complement(13896..15290)
                     /gene="bglB"
                     /locus_tag="ACHK74_001205"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFP0220063.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLEAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(15309..17186)
                     /gene="bglF"
                     /locus_tag="ACHK74_001206"
     CDS             complement(15309..17186)
                     /gene="bglF"
                     /locus_tag="ACHK74_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFP0220064.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(17319..18155)
                     /gene="bglG"
                     /locus_tag="ACHK74_001207"
     CDS             complement(17319..18155)
                     /gene="bglG"
                     /locus_tag="ACHK74_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFP0220065.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(18441..19166)
                     /gene="phoU"
                     /locus_tag="ACHK74_001208"
     CDS             complement(18441..19166)
                     /gene="phoU"
                     /locus_tag="ACHK74_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFP0220066.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(19181..19954)
                     /gene="pstB"
                     /locus_tag="ACHK74_001209"
     CDS             complement(19181..19954)
                     /gene="pstB"
                     /locus_tag="ACHK74_001209"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP0220067.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(20045..20935)
                     /gene="pstA"
                     /locus_tag="ACHK74_001210"
     CDS             complement(20045..20935)
                     /gene="pstA"
                     /locus_tag="ACHK74_001210"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFP0220068.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(20935..21894)
                     /gene="pstC"
                     /locus_tag="ACHK74_001211"
     CDS             complement(20935..21894)
                     /gene="pstC"
                     /locus_tag="ACHK74_001211"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFP0220069.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(21981..23021)
                     /gene="pstS"
                     /locus_tag="ACHK74_001212"
     CDS             complement(21981..23021)
                     /gene="pstS"
                     /locus_tag="ACHK74_001212"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFP0220070.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(23269..24342)
                     /gene="lpfD-O113"
                     /locus_tag="ACHK74_001213"
     CDS             complement(23269..24342)
                     /gene="lpfD-O113"
                     /locus_tag="ACHK74_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFP0220071.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(24353..26875)
                     /gene="lpfC-O113"
                     /locus_tag="ACHK74_001214"
     CDS             complement(24353..26875)
                     /gene="lpfC-O113"
                     /locus_tag="ACHK74_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFP0220072.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGIGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(26900..27631)
                     /gene="lpfB-O113"
                     /locus_tag="ACHK74_001215"
     CDS             complement(26900..27631)
                     /gene="lpfB-O113"
                     /locus_tag="ACHK74_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFP0220073.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(27679..28251)
                     /gene="lpfA-O113"
                     /locus_tag="ACHK74_001216"
     CDS             complement(27679..28251)
                     /gene="lpfA-O113"
                     /locus_tag="ACHK74_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFP0220074.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            complement(28553..30382)
                     /gene="glmS"
                     /locus_tag="ACHK74_001217"
     CDS             complement(28553..30382)
                     /gene="glmS"
                     /locus_tag="ACHK74_001217"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFP0220075.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(30544..31914)
                     /gene="glmU"
                     /locus_tag="ACHK74_001218"
     CDS             complement(30544..31914)
                     /gene="glmU"
                     /locus_tag="ACHK74_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFP0220076.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(32265..32684)
                     /gene="atpC"
                     /locus_tag="ACHK74_001219"
     CDS             complement(32265..32684)
                     /gene="atpC"
                     /locus_tag="ACHK74_001219"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFP0220077.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(32705..34087)
                     /gene="atpD"
                     /locus_tag="ACHK74_001220"
     CDS             complement(32705..34087)
                     /gene="atpD"
                     /locus_tag="ACHK74_001220"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFP0220078.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(34114..34977)
                     /gene="atpG"
                     /locus_tag="ACHK74_001221"
     CDS             complement(34114..34977)
                     /gene="atpG"
                     /locus_tag="ACHK74_001221"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFP0220079.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YADTMRKVIGHLASGNLEYKHPYLEERDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(35028..36569)
                     /gene="atpA"
                     /locus_tag="ACHK74_001222"
     CDS             complement(35028..36569)
                     /gene="atpA"
                     /locus_tag="ACHK74_001222"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFP0220080.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(36582..37115)
                     /gene="atpH"
                     /locus_tag="ACHK74_001223"
     CDS             complement(36582..37115)
                     /gene="atpH"
                     /locus_tag="ACHK74_001223"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFP0220081.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(37130..37600)
                     /gene="atpF"
                     /locus_tag="ACHK74_001224"
     CDS             complement(37130..37600)
                     /gene="atpF"
                     /locus_tag="ACHK74_001224"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFP0220082.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(37662..37901)
                     /gene="atpE"
                     /locus_tag="ACHK74_001225"
     CDS             complement(37662..37901)
                     /gene="atpE"
                     /locus_tag="ACHK74_001225"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFP0220083.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(37948..38763)
                     /gene="atpB"
                     /locus_tag="ACHK74_001226"
     CDS             complement(37948..38763)
                     /gene="atpB"
                     /locus_tag="ACHK74_001226"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFP0220084.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(38772..39152)
                     /gene="atpI"
                     /locus_tag="ACHK74_001227"
     CDS             complement(38772..39152)
                     /gene="atpI"
                     /locus_tag="ACHK74_001227"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFP0220085.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(39769..40392)
                     /gene="rsmG"
                     /locus_tag="ACHK74_001228"
     CDS             complement(39769..40392)
                     /gene="rsmG"
                     /locus_tag="ACHK74_001228"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFP0220086.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(40456..42345)
                     /gene="mnmG"
                     /locus_tag="ACHK74_001229"
     CDS             complement(40456..42345)
                     /gene="mnmG"
                     /locus_tag="ACHK74_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFP0220087.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(42723..43166)
                     /gene="mioC"
                     /locus_tag="ACHK74_001230"
     CDS             complement(42723..43166)
                     /gene="mioC"
                     /locus_tag="ACHK74_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFP0220088.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(43256..43714)
                     /gene="asnC"
                     /locus_tag="ACHK74_001231"
     CDS             complement(43256..43714)
                     /gene="asnC"
                     /locus_tag="ACHK74_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFP0220089.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            43866..44858
                     /gene="asnA"
                     /locus_tag="ACHK74_001232"
     CDS             43866..44858
                     /gene="asnA"
                     /locus_tag="ACHK74_001232"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFP0220090.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(44863..46314)
                     /gene="viaA"
                     /locus_tag="ACHK74_001233"
     CDS             complement(44863..46314)
                     /gene="viaA"
                     /locus_tag="ACHK74_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFP0220091.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(46308..47804)
                     /gene="ravA"
                     /locus_tag="ACHK74_001234"
     CDS             complement(46308..47804)
                     /gene="ravA"
                     /locus_tag="ACHK74_001234"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFP0220092.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            48027..49895
                     /gene="kup"
                     /locus_tag="ACHK74_001235"
     CDS             48027..49895
                     /gene="kup"
                     /locus_tag="ACHK74_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFP0220093.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            50062..50481
                     /gene="rbsD"
                     /locus_tag="ACHK74_001236"
     CDS             50062..50481
                     /gene="rbsD"
                     /locus_tag="ACHK74_001236"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFP0220094.1"
                     /translation="MKKGTVLNSDISSMISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            50489..51994
                     /gene="rbsA"
                     /locus_tag="ACHK74_001237"
     CDS             50489..51994
                     /gene="rbsA"
                     /locus_tag="ACHK74_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFP0220095.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLASGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            51999..52964
                     /gene="rbsC"
                     /locus_tag="ACHK74_001238"
     CDS             51999..52964
                     /gene="rbsC"
                     /locus_tag="ACHK74_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFP0220096.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            52989..53879
                     /gene="rbsB"
                     /locus_tag="ACHK74_001239"
     CDS             52989..53879
                     /gene="rbsB"
                     /locus_tag="ACHK74_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFP0220097.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            54005..54934
                     /gene="rbsK"
                     /locus_tag="ACHK74_001240"
     CDS             54005..54934
                     /gene="rbsK"
                     /locus_tag="ACHK74_001240"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP0220098.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            54938..55930
                     /gene="rbsR"
                     /locus_tag="ACHK74_001241"
     CDS             54938..55930
                     /gene="rbsR"
                     /locus_tag="ACHK74_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFP0220099.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(55896..57323)
                     /gene="mdtD"
                     /locus_tag="ACHK74_001242"
     CDS             complement(55896..57323)
                     /gene="mdtD"
                     /locus_tag="ACHK74_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFP0220100.1"
                     /translation="MSDKKNRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLTDLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(57346..58038)
                     /gene="yieP"
                     /locus_tag="ACHK74_001243"
     CDS             complement(57346..58038)
                     /gene="yieP"
                     /locus_tag="ACHK74_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFP0220101.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT        14441 a        14712 c        14197 g        14801 t
ORIGIN      
        1 cgaggataag tgcattatga atatcttaca tatatgtgtg acctcaaaat ggttcaatat
       61 cgacaataag attgtcgatc accgccctta atttaccctt ctgtagccat caccagagcc
      121 aaaccgatta gattcaatgt gaacgattag tttgctatat cttaattttg ccttttgcaa
      181 aggccatctc tcgtttattt acttatttta gtaaatgatg gtgcttgtat atatacatgg
      241 cgaattaatg gatattgcag atgtaatatt cacagggatc actgtaatta aaataaatga
      301 aggattatgt aatggaaaac tttaaacatc tccctgaacc gttccgcatt cgtgttattg
      361 agccagtaaa acgtactact cgcgcttatc gtgaagaagc aattattaaa tccggtatga
      421 acccgttcct gctggatagc gaagatgtgt ttatcgattt actgaccgac agcggcaccg
      481 gggcggtaac ccaaagtatg caggcagcga tgatgcgcgg cgacgaagcc tacagcggca
      541 gccgcagcta ctatgcgtta gccgagtcag tgaaaaatat ctttggttat caatatacta
      601 ttccgactca ccagggccgt ggcgcagagc aaatctatat tccggtactg attaaaaaac
      661 gcgagcagga aaaaggcctg gatcgcagca aaatggtggc attctctaac tatttctttg
      721 ataccacgca gggccatagc cagattaacg gctgtaccgt gcgtaacgtc tatatcaaag
      781 aagccttcga tacgggcgtg cgttacgact ttaaaggcaa ctttgacctc gaaggattag
      841 aacgcggtat tgaagaagtt ggcccgaata acgtgccgta tatcgttgca accatcacca
      901 gtaactctgc aggtggtcag ccggtttcac tggcaaactt aaaagcgatg tacagcatcg
      961 cgaagaaata cgatattccg gtggtaatgg actccgcacg ctttgctgaa aacgcctatt
     1021 tcatcaagca gcgtgaagca gaatacaaag actggaccat cgagcagatc acccgcgaaa
     1081 cctacaaata tgccgatatg ctggcgatgt ccgccaagaa agatgcgatg gtaccgatgg
     1141 gcggcttgct gtgcatgaaa gacgacagct tctttgatgt gtacaccgag tgcagaaccc
     1201 tttgcgtggt gcaggaaggg ttcccgacat atggcggcct ggaaggcggc gcgatggagc
     1261 gtctggcggt aggtctgtat gacggcatga atctcgactg gctggcttat cgtatcgcgc
     1321 aggtacagta tctggtcgat ggtctggaag agattggcgt tgtctgccag caggcgggcg
     1381 gtcacgcggc attcgttgat gccggtaaac tgctgccgca tatcccggca gaccagttcc
     1441 cggcacaggc gctggcgtgc gagctgtata aagtcgccgg tatccgtgcg gtagaaattg
     1501 gctctttcct gttaggccgc gatccgaaaa ccggtaaaca actgccatgc ccggctgaac
     1561 tgctgcgttt aaccattccg cgcgcaacat atactcaaac acatatggac ttcattattg
     1621 aagcctttaa acatgtgaaa gagaacgcgg cgaatattaa aggattaacc tttacctacg
     1681 aaccaaaagt attgcgtcac ttcaccgcaa aactgaaaga agtttaatta atactacaga
     1741 gtggctataa ggatgttagc cactctctta ccctacatcc tcaataacaa aaatagcctt
     1801 cctctaaagg tggcatcatg actgatcaag ctgaaaaaaa gcactctgca ttttggggtg
     1861 ttatggttat agcaggtaca gtaattggtg gaggtatgtt tgctttacct gttgatcttg
     1921 ccggtgcctg gtttttctgg ggtgccttta tccttatcat tgcctggttt tcaatgcttc
     1981 attctgggtt attgttatta gaagcaaatt taaattatcc tgtcggctcc agttttaaca
     2041 ccatcaccaa agatttaatc ggtaacacct ggaacattat cagcggtatt accgttgcct
     2101 tcgttctcta tatcctcact tatgcctata tctctgctaa tggtgcgatc attagtgaaa
     2161 cgatatcaat gaatttgggc tatcacgcta atccacgtat tgtcgggatc tgcacagcca
     2221 ttttcgttgc cagcgtattg tggataagtt cgttagccgc cagtcgcatt acctcattgt
     2281 tcctcgggct gaagattatc tcctttgtga tcgtgtttgg ttctttcttc ttccaggtcg
     2341 attactccat cctgcgcgat gccaccagca ccactgcggg aacgtcttac ttcccgtata
     2401 tctttatggc tttgccggtg tgtctggcgt catttggttt ccacggcaat attcccagcc
     2461 tgattatttg ctatggaaaa cgcaaagata agttaatcaa aagcgtggta tttggttcgc
     2521 tgctggcgct ggtgatttat ctcttctggc tctattgcac gatggggaat attccgcgcg
     2581 aaagctttaa ggcgataatc tcctcaggcg gcaacgttga ttcgctggtg aaatcgttcc
     2641 tcggcaccaa acagcacggc attatcgagt tttgcctgct ggtgttctct aacttagctg
     2701 ttgccagttc gttctttggt gtcacgctgg ggttgttcga ttatctggcg gacctgttta
     2761 agattgataa ctcccacggc gggcgtttca aaaccgtgct gttaaccttc ctgccacctg
     2821 cgttgttgta tctgatcttc ccgaacggct ttatttacgg gatcggcggt gccgggctgt
     2881 gcgccaccat ctgggcggtc attattcccg cagtgcttgc aatcaaagct cgcaagaagt
     2941 ttcccaatca gatgttcacg gtctggggcg gcaatcttat tccggcgatt gtcattctct
     3001 ttggtataac cgtgattttg tgctggttcg gcaacgtctt taacgtgtta cctaaatttg
     3061 gctaaatcct tcaagaagcc agccattcgc tggcttcttg cctctcagga aatcacttat
     3121 gtccaaatgg caactcgcct gatcctcctt caccacgtat gctttgcgtc accttactat
     3181 caggacgctt tagcccatgt cccgcttttt gatttgtagt tttgccctgg ttttacttta
     3241 tcccgccggg attgatatgt acctcgttgg tttaccgcgc atcgccgccg atctcaatgc
     3301 cagcgaagcg cagttgcata ttgcgttctc cgtatatctg gcggggatgg cagctgcgat
     3361 gttatttgcc ggtaaagtgg ccgatcgttc agggagaaag ccggtcgcca tacccggcgc
     3421 ggcgctattt attattgcct cggtgttctg ttcactggct gaaaccagca cgttatttct
     3481 tgcaggccga tttctacagg ggttgggcgc aggctgttgt tacgtagtgg cgttcgccat
     3541 tttgcgcgac acgctggatg atcgacgtcg ggctaaagtg ctgtcattac tcaacggtat
     3601 tacctgcatc attccggtgt tagcgccagt gctcggacat ctgattatgc ttaaattccc
     3661 gtggcagagt ctgttctggg cgatggcaat gatgggcatc gcggtactga tgttgtcttt
     3721 gtttatttta aaagaaacgc gcccagcgtc ccccgctgct tcggacaaac cacgagaaaa
     3781 tagcgagtcg ctgcttaatc ggtttttcct cagccgtgtt gttatcacca ccctcagcgt
     3841 ttcggtgatc ctcactttcg tcaatacatc gccggtattg ctgatggaaa tcatgggttt
     3901 tgagcgcgga gaatacgcca ccattatggc gttgactgct ggcgtcagca tgaccgtttc
     3961 attctccacg ccatttgcgc tgggaatttt taagccacgt acgttgatga tcacctcgca
     4021 ggtgttattc cttgcagcgg ggatcaccct tgccgtttca ccttcccatg cggtttctct
     4081 gtttggtatc acgctgattt gcgccggttt ctcggtaggt tttggcgtag cgatgagtca
     4141 ggcgttagga ccattttcat tacgcgcggg cgtagccagc tcgaccttag gtattgcgca
     4201 ggtttgcggt tcgtcactgt ggatttggct ggcagcggtg gttggtatcg gcgcatggaa
     4261 tatgctgatc gggattctga ttgcctgtag catagtgagc ctgttgctga ttatgttcgt
     4321 cgcgcctgga cgccccgttg ccgctcatga agaaatccat caccacgctt gatctcaatc
     4381 tgctgctctg tctgcaactg ctgatgcagg agcgcagcgt gaccaaagcg gcgaagcgga
     4441 tgaacgtgac cccttcagcg gtgagtaagt cgctggcaaa gttaagagcg tggtttgacg
     4501 acccgctctt tgtgaactca ccgctgggcc tgtcgcccac accgctaatg gtcagcatgg
     4561 agcaaaatct ggcggaatgg atgcaaatga gcaatctgct gctggataaa ccgcaccacc
     4621 agacaccgcg cggcctgaag tttgagttgg cggcggaatc gccgctgatg atgatcatgc
     4681 ttaatgcgct gtcgaaacgg atctaccaac gttacccgca ggcgaccatc aaattacgta
     4741 actgggatta cgattcctta gatgccatta ctcgtggtga agtggatatt ggtttttccg
     4801 gtcgcgaaag ccatcctcgc tcgcgggagc tgttaagctc gctaccgtta gccattgatt
     4861 atgaagtgct gtttagtgat gtgccctgcg tctggttacg caaagatcat ccggcactgc
     4921 atgaaacgtg gagtctggac acttttttgc gttatccgca tatcagcatt tgctgggaac
     4981 agagcgatac ctgggcgctg gacaatgtgt tacaggagct ggggcgcgaa cgcacgattg
     5041 ctatgagcct gccggaattc gagcagtcac tgtttatggc agcgcagccc gacaatctgc
     5101 tactggcgac cgcgccgcgc tactgtcagt actacaatca actccaccaa ctgccgttgg
     5161 ttgcgcttcc tctcccgttt gacgaaagcc agcaaaaaaa gctggaagtc ccttttaccc
     5221 tgctttggca taaacggaac agccataatc cgaagatcgt ctggttacgg gaaaccatta
     5281 aaaatcttta cgcgtcgatg gcataaccga atcgtatgaa acgcgaccct atttcacaat
     5341 aaaatgtaaa aattttgtaa taaacttgtc tgaatcgaac ttttagccgc tttagtctgt
     5401 ccatcattcc agtaaatgat tactcttgta ttcataatgg accattaagc atggagcgaa
     5461 aaatggcgac tcactttgcc cgagggattt taacggaagg acatctgatt tctgttcgtc
     5521 tcccctccca gtgtcatcaa gaagcccgaa acattcccct tcatcgtcaa agccgttttc
     5581 tggcgtccag aggtttactc gcagaactga tgttcatgct gtatggcatt ggcgaattgc
     5641 cggaaattgt caccctgccg aaaggtaaac cggttttcag cgataaaaat ttgccttcgt
     5701 tttctatttc ctatgccggg aatatggttg gcgtggcgtt aacaaccgaa ggtgaatgtg
     5761 gcctcgatat ggaactacag cgtgcgacgc gcgggtttca tagcccacac gcgcccgata
     5821 accacacctt ttcaagcaat gaatcgctat ggatcagtaa acaaaacgat cctaacgaag
     5881 cgcgggcgca gctcatcacg ctgcgccgaa gcgtgctaaa actaaccggt gatgttttga
     5941 atgacgatcc gcgcgatctg cagctgctgc ccattgccgg acgcctgaaa tgtgctcatg
     6001 taaatcatgt agaagcgtta tgcgacgcgg aagacgtgct ggtgtggtcc gtggcggtca
     6061 cgcccacgat tgaaaagctc agtgtctggg agttagatgg caaacacggc tggaaaagcc
     6121 tgccggatat tcacagccgc gccaacaatc ctaccagccg gatgatgcgt tttgcccagc
     6181 tctctaccgt gaaggctttt tcgccaaatt gatagacaac cacaggagtc atcatgtctg
     6241 aaaaattgaa ggtggttacg ttactgggga gcctgcgcaa aggctcattt aatggcatgg
     6301 ttgcacgtac cctgccgaaa attgctccgg cgagcatgga agtcaatgcg ttaccttcca
     6361 ttgccgacat tcccttgtat gacgctgacg tacagcagga agaaggtttt ccagcaacgg
     6421 tagaagctct ggcggaacag atccgtcagg ctgacggtgt ggtgattgtc acgccggaat
     6481 ataactactc ggttccgggc ggtctgaaaa atgccatcga ctggctttcc cgcctgccgg
     6541 atcaaccgct ggccggtaaa ccggtgttga ttcagaccag ctcaatgggc gtgattggcg
     6601 gcgcgcgctg tcagtatcac ctgcgccaga ttctggtttt cctcgatgca atggtgatga
     6661 acaagccgga atttatgggc ggcgtgattc agaacaaagt tgatccgcaa accggagaag
     6721 tgattgatca gggtacgctg gaccacctga ccgggcaatt gaccgcattt ggtgagttta
     6781 ttcagcgagt taagatctaa ataaaaaacc cgccagcaat catgcatggc gggtttttaa
     6841 cgcgctatcg attttagtga gcgtcgataa acacaatctt caggataaac agcagcgcaa
     6901 cgatgattac gcacgggctt agatcacgca gacgtccggt accgattttc atcacgcagt
     6961 aggagataaa gcccagcgcg ataccttcgg taatcgagaa gctgaacggc atcatcacgg
     7021 cggtaataaa cgccggaaca gattcagtaa gatcctgcca gttcacgcgt gccagactgg
     7081 aagtcatcag cacgccaacg taaatcagcg caccagctgc agcgtagcct ggcaccatcc
     7141 ccgccagcgg cgacagaaag ataaccagca ggaacagcag accaacaacc actgccgtca
     7201 gaccggtacg accgccaacc gatacgccgg aagaggactc aatataagcc gtaacggaag
     7261 aagtaccgat aaacgaaccg gtcacggaag agatactgtc gacatacagc gcctgcttca
     7321 tgcgcgggaa tttacccttc tcatccgcca ggcctgcttt atcggtcacg ccaatcagcg
     7381 taccggagga gtcaaacagg ttgaccagca tgaaagagaa tatcacccct gccagcccga
     7441 ggttaaacga cccggctaaa tctacatggc ccacgacagt cattacgctc ggcggcgcag
     7501 aaacgatgcc attgtagtgg acattaccca gcatccagcc cagcagcgtt gtcaccacga
     7561 tggaaaccag caccgccgcg tgaatgttgc gcgaggccag aatagcgatg atgaagaagc
     7621 cgaggatccc cagcagcacg ctgtgagaag tcagattacc gatgctgacc agcgtttccg
     7681 ggttagcgac aatcacacct gcattcttca gtcccatcat accaatgaac agaccgatac
     7741 cgctggtgat gcccacacgc agactcaccg gaatgttggc aatcatccag tagcgaacgc
     7801 ggaaaatcgt cagtaacagc agacctatcg cgccccagaa gattgcgccc atcccgactt
     7861 gccacggcaa gcccatcgcc tgcacaacga caaaagcgaa gaatgcattc aggcccattg
     7921 ccggtgccag tgcaactggc aggttagcaa acagtcccat cataatactg ccgaatgcag
     7981 caatcagaca ggtagtgacg aagacggcgc tggtatccat gccagcaacg ccaagaattt
     8041 gcgggttaac aaaaacgatg taaaccatcg tcaggaaggt ggtaaaaccg gcgatcactt
     8101 cggtccgtgc cgtcgtgcca tgttcgcgca gtttaaacac gcgttccagc atcccctggc
     8161 cagaagcctg ggttgtgtgt tgttgactca ttatctattt ccgaacaagg agggaaaatc
     8221 cgtcgctatc gtataccaaa atgagacaat atggacggct gaagataact ttttttcatt
     8281 gtatttgctt atacggcaac gattgcgtca tgcgaattgg catgacgaaa acgttaaact
     8341 aacgataaag gaaagacatg tcccagatag aagcggtatt tttcgactgc gacggtacgc
     8401 tggtcgacag tgaagtcatt tgctcccgcg catatgtaac gatgtttcag gaatttggta
     8461 ttacgctcga tcctgaagag gtattcaaac gtttcaaagg tgtaaaactg tacgaaatta
     8521 tcgatattgt ttcccttgaa catggtgtta cgttagcgaa aacagaagct gaacacgttt
     8581 accgtgcaga agtcgctcgg ctgttcgatt cagaactgga agccatcgaa ggggctggag
     8641 cgctcctgtc agcgatcact gcgccaatgt gtgtggtatc taacggccca aataacaaaa
     8701 tgcagcattc tatgggcaag ctgaatatgt tgcactactt cccggataaa ctgttcagcg
     8761 gctacgatat tcagcgctgg aagccagacc cggcgttaat gttccatgcg gcaaaagcga
     8821 tgaatgtaaa tgtagaaaac tgcattctgg ttgatgactc agttgccggt gcgcaatctg
     8881 gtatcgacgc aggtatggaa gtgttctact tctgcgccga cccgcacaat aagccgatcg
     8941 ttcacccgaa agtcaccacc tttacccatc tttcgcagtt accggaactg tggaaagcgc
     9001 gtggttggga tattacggca tagttcttca cactcccttc acttaccccg cttaaattgg
     9061 cgctcaaagg taagtaaagg gtgtttgata tgtctgtttc acgtcgggta atacatcacg
     9121 gactttattt tgcggtttta ggaccgttaa ttggtgttct gtttcttgtc ctctacatat
     9181 tcttcgcaaa agaaccgctg gttcttttgg taataataca ggtacttcca ttgtttctct
     9241 tattgtcgat tactacggga gctattcctg cattgttaac cggcgtgatg gtcgcctgtc
     9301 tgccggaaaa gatcgggtca cagaaaaatt atcgttgtct ggctggtggc ataggtggcg
     9361 tcgttatcac cgagatctat tgtgcagtta ttgtacatat taagggcatg gcttcctcgg
     9421 agttgtttga aaacattctt tctggtgaca gtctcgttgt ccgcatcatt cctgcattgc
     9481 tggcaggtgt ggtgatgagc agaatcatta cccgtctgcc cggattggat atttcatgtc
     9541 ctgaaacaga ctctttaagt taagcgggat actttatctt tgggctactc aaaagcagac
     9601 aggatgttac tatgactcaa aatatcaggc cgttacccca attcaaatat catcccaagc
     9661 cactggaaac aggcgcattt gaacaggata aaaccgtaga gtgcgattgc tgtgaacaac
     9721 agacgtcagt ttattactcg ggtccctttt attgcgttga tgaagttgaa catctctgtc
     9781 cgtggtgtat tgcggacggt tctgctgctg aaaagtttgc aggtagtttt caggatgatg
     9841 ccagcataga aggtgttgaa tttgagtatg atgaagagga cgaatttgcc ggtattaaga
     9901 acacatatcc tgatgaaatg ctgaaagagt tggttgaacg cacgccaggt tatcatggat
     9961 ggcaacagga attctggctt gcgcattgtg gcgatttctg tgcttttatc ggctatgtgg
    10021 gctggaatga tataaaagat cgcctcgatg aatttgccaa ccttgaagaa gattgtgaga
    10081 atttcggtat tagaaattct gatctagcta aatgcctgca aaagggtggt gattgtcagg
    10141 gttatctctt ccgctgtctc cactgcggca agctgagact gtggggtgat ttttcgtagt
    10201 tatttaaata atgagaacag gccggagcgt aattcataca tccggcctta tttcttcagc
    10261 ttaaccgagc gctaattctg cggcagcggc tttatcagcg attaccatca gcgaaggatg
    10321 cagctgcaac acggaagctg gaacatcttc ggtaaccgga ccttgcagca catttttcag
    10381 tgcctgcgct ttacctgcgc cactgacaat tatcagcaag tttttcgccg ccatgatgct
    10441 tttcggcccc atcgtcacgt agctgtcagg cactaacgaa aagtcgccgc ccaactcacc
    10501 atgcgccaca atatcaacca tctctccctg gatcgggaac tccacggttt gctcatggaa
    10561 atgggtcgta ttcggcaaat tgccacaaaa atgaccatct gcacctaatc ccagcaccac
    10621 caaatccagg ccgccttcac gcgccagttt ctgatcatgc tcgcggtagt tatcaatagt
    10681 gagcttctgg atattctctt ctttgatccc cgcaggggtg aaaaacagat tacgcagatt
    10741 ggtaatcgtt acaccttctc cctctttgcc gcgaaaggga atttcatcga aattatagaa
    10801 atagcagtta tcgtaccagg gcttaccttt aaccagggtg gtgaggtatt catacatgcc
    10861 tttgggcgtg ctaccggcgg taattgccag gttaacacga cgcgtcttcg acatataacc
    10921 aagcagatgg tgtgccgcga cacggctcat ttcctggtaa tcttcggtaa tgattaattt
    10981 catcttagtg ccttatttta tttaaataat ttctgtacga aggctgcata ggcaggccgc
    11041 cagacatcca tttcatggtt caggccggga tattcctggt aatcaaactt aatttttttc
    11101 tgctcaagct cagttttcag cccggcgata tccttgccgg ttacgacatc tttatcacca
    11161 acgaccacag taaaattacg tagttgctgg ttaatagctg ccggatcgtt cagccgggcc
    11221 gcgacacctt catccggtac ggttgtcgtg gtaacaccac tgaatgtggc cagccagcca
    11281 aagctttcca gatgattcat tccggaaacc agcgcctggt acccgccttg tgaaagccct
    11341 gccagcgcgc gaccatcggc atctttacgg acattaaaac gcttgctaat cagcgggata
    11401 atatcgttca tcagttcgcg atctgccgct ttagcattca gcggataaaa gactttacgt
    11461 ctttcctgag gcacgaaatc ttcgggaata atgcccttcg catcggtttc ggtatctggg
    11521 atcaccacca gcatcggttt aattttccct tcagccagca ggttatccat gatttgcggg
    11581 atacgcccct gatcgatagc ggaacgtcca gtatcaccaa agccgtgata gaaatagagc
    11641 actggcaaag gctcgcccat gccggtgtat cctggcgggg tccagacata catctgacgt
    11701 tcagattgca atgcgttgga gtggtaagtt atggcgatca aatcaccatg cgcaacagaa
    11761 cgcgtatcca gataactgcc tggcaccaga atcatactgg agttaacctg gcgctgaggc
    11821 ttagtcattg cggtgccagt atcaatgctg cgtacaccat caacattgaa aaaatattcg
    11881 tacagattgc ctttcaggac aggtgtgcgc cacgaccaga ctcccgcttc gtctttggtc
    11941 atcgggtgaa tattgtccgg aaccggaaca cccaccacta cggagacgtt ttttgcccca
    12001 ggggcaaagt agcgaaaggt cacgctgtta tcggcattga cctgagtcac atactgttta
    12061 accggaatag tgggtgccgg gctggctggc atatccgctg ctatggccca ctgagcagaa
    12121 ataccgctgg caataaccag cgttaaagca gcaattttta tattcatcgt aatattcctt
    12181 tcattaagtt ccattaccac cagatttcag cctgggcacc gacagcaaac tggtcgtctt
    12241 tattgtcttc aaaggtgaag ccatccagtt cgttttccag ggctttgata tacgtggcgt
    12301 agaaacgaat ttccggacgc gaggtcaaca tactggtatt gactttaaag gtatggaaga
    12361 gcgtggtttt ataaccagac tcattaaatt tattactatt cgcatctttg ttttgctggg
    12421 tgaaatagcc cagttcaaca cctgtctggt tatattggtc ccaaatatag gccgggcgaa
    12481 caaccgcacg aatagattcg aaatcagagt gggcgcctgt ttcgtagcta tatatatcgt
    12541 taccgaagga gtaaacaatc gcgttagcta caatgaaatg atcgccaata taggcttcgc
    12601 cctgcgaagt cagacgtacc gctgttccgc cggtgagatc accataataa cgaccattaa
    12661 aggtggtaaa tggacttgcg ccagcataac ggccaaagtt actggcgata gaattattcg
    12721 cgaccaggaa ggagaattcg ttgaagccac ctttatcaaa tttctgcgtt aaagatgtgc
    12781 caaacatcca ggtatctttc cagtcataat agccgttatt atcctgatta tctttttccg
    12841 atgcgctttc gtttgccgtg acataacgac cactcaccat taaggtcgct ttatcccata
    12901 acgggatatc tttatagcgt aaatcaatag tattggtatt aatctgctgt ttgttttgca
    12961 ggctgcgatc gtaatcatcg acatcttcgc gaaccagcgc gatatcaatt ttacccggac
    13021 cgactttcca gttttccaga cctacacccg ctgcggcatc agtacgctgc gttttccagt
    13081 ccagcatctg gatttcaatt ttcggcgcac cgtgtttacc cacccagaaa tcagcctctg
    13141 gcgcaaaggg caggaaacct ttggtggtaa cgtacatatc ggagaactgc ataaagttct
    13201 cgccaccggc gttatcgcca aaccagccgg tagagtactg ctgaccaacg ttaccatcca
    13261 tcatcacaac ggcatcaacc cgtttgccgt tttcgttgta gacacgttgt tttaactgca
    13321 aatcaaacca gccggagtat tcgttaccaa agcggcccag agaaccaatc gcccatgatt
    13381 taggtgaacc atgagaggcg gtcccccaac cagaacggta atagccgttg taactaaagc
    13441 caatttcatc tttcacaaac ttactgaaat ctttcagagt catggaagaa taaatcgagc
    13501 ctgtctcact ggcatttttc tcttcatttt tgaccagtac agcatcaggt tttgcggcac
    13561 tactggtcgg gaacggattg gcggcatacc cttgatcatt cgtacttacg ctacgattca
    13621 ccgtcgctgg cgtatatttt ttcttctctt catctttata ctttttgagt tcgctttgcg
    13681 tttctcttaa cgctttttct aataactcaa ggcgatgttc caccgttaat gattcagcca
    13741 atgattgtgc agaaaaggct aacggtgcca ttaataagat ggcagaggta ataagatttc
    13801 gtctaaacat aatatccctt tatggtgcaa agaaagaatt aacgtatcgc atccagactg
    13861 ttctgaatgc gacgataatt aaggtgcttt attggttatt tttttaatga cagcccccgc
    13921 gttttgatca cttctgcata ccatccgaag cttttcttac gcgttcttgt gaggcttcct
    13981 tcgccattat catcacgatc cacataaata aagccgtagc gcttagacat ttgtgaatga
    14041 gatgcactga ctaaatcaat tggcccccaa ctggtgtacc ccataatatc cacaccatcg
    14101 gcaatcgctt catttacctg taccaggtga tcgtttaaat aggcaattcg ataatcgtcc
    14161 tgtatcgaac catccgcttc aacgctgtct tttgcgccta atccgttctc gacaataaat
    14221 aacggttttt gataacgatc ccaaagcgta tttaacagaa cccgtaatcc aaccggatca
    14281 atttgccacc cccactctga acttttcaga tgcggattgg ggatcatatt cagtatgttg
    14341 ccctgcgcat ttttattaat gctttcgtcg tgggaaacac aaccagtcat gtaataacta
    14401 aaagagatga aatctacggt atgttttaaa tcttctgcgt cactttcagt catctcaatg
    14461 gtgatattgt ggtcgcggaa gaaacgctgc atatagccgg gatactgacc acgcgcctga
    14521 acatcaccaa agaacatcca gcgccggttc tcttccatgg cctccaacat atcctgtggc
    14581 tggcaggtga gggggtaaac cagcccgccg agaagcatat tgccgatttt cgcttcgggg
    14641 atcaggctat gacaggcttt aactgcccgc gcactggcaa ccagttgatg atggatcgcc
    14701 tgataaactt ccgcctcgcc actctcttct gccagcccca cgcccgtgaa tggcgcgtgt
    14761 aacgacatat tgatttcatt aaacgtcagc cataacgcta ctttatgttg gtagcgggta
    14821 aagaccgtgc gggcgtaatg ctcgaagtga tcgatgaccg cccgattagc ccaaccgccg
    14881 tagtttttca ccagcccata tggcatttcg taatgggata acgttaccag cggcttgatc
    14941 cccgcctgcg ccatttcatc aaacagccga tcgtaaaacg ctaaccccgc ttcattcggt
    15001 tcggcttcgt cgccctgagg gaaaattcgc gcccaggcaa tggaaatacg cagacaggtg
    15061 aagcccatct cggcaaataa cgcgatatct tccgggtaac ggtgataaaa atcgatggcg
    15121 acatctttga tattctcttt ccccaggatg cgcggttcca tttttcccat tacgccatga
    15181 ggctgtaaat ctgaggtcgt gatccctttg ccatcttcca gccaggcacc ttccacctga
    15241 ttggcagctg ttgcgccacc ccaaagaaat gtttctggaa atgctttcat aattaactcc
    15301 ttttatcgtt agcgaatgat ggataacagc ggctcaccag cgcttatctg cgccgtgccg
    15361 tggggtaata cgtctgtaaa atcatcgcta ttactgatta ataccggcgt cgtcagatca
    15421 aatccggcct cgcgaatagc agggatatca aaagaaatca gccgatcgcc tgtattgacc
    15481 ttgtcaccca cgttgacgtg agcggaaaag aatttgccgt ccagttttac ggtgtcgata
    15541 ccgacatgaa tcaggatctc cacaccatca tctgactcaa tgccaatagc gtgtaatgtg
    15601 gcgaacaacg aagcaattcg acccgcaacc ggagaacgca cttcaccaac cgagggcaga
    15661 atggcgatac ctttacccaa caggccactg gcaaacgtgg tatcagcgac gtgaatgagc
    15721 ggcacaatct ctcccgtcat cggtgaacag ataccgccct gctcaggtgg tgtaataacc
    15781 tctggtgttt tctcttgcgg ggcaccctgc gctggctgac gtttagcggt gatgaaatga
    15841 agcatcaccg taccgacaaa tgcgcaaccg atggcaatga caccgccaat aacgctggcc
    15901 cagacggtga aatcaattcc cgttgacggg atggtttgca tgaaggtgaa aatacttggc
    15961 aaaccaaagg agtacacttt cgtttgcgcg tagccaatga tggtggcccc caaagcccca
    16021 ctgatacagg cgataacaaa ggggtactta cgcggcagat taacgccata taccgctggt
    16081 tcggtgatac caaacaggcc cgtcaacgcc gctgatcccg ccaccacttt tttctgcgca
    16141 tcgcgttcgc agaggaagac gccgagcgcc gccccgacct gcgccataat ggcgggcatt
    16201 aacagcggga tcatggtgtc gtagcccagc acggtgaagt tattgataca cagcggcacc
    16261 aggccccagt gcagtccgaa catgacgaag atttgccaga agccgcccat taccgcgccc
    16321 gcaaatgcag gaaccgcctg ataaagccag agataaccgg cggcaatcag ttcgcttatc
    16381 caggttgata gcggccccac cagcagaaag gtgacgggtg tgataaccat cagacatagc
    16441 aatggtgtga agaaattttt gattgccgac ggtaaccacg cattaagtcg gcgttccaga
    16501 atgctgcaca accaggcaga aaaaataatg ggaataaccg atgacgagta attcaacaat
    16561 gtgaccggaa tacccaggaa atccagcccc agctcatccg ctttttgccc gttctcgaaa
    16621 gcagtcagaa ttaatggatg cactaacgct ccaccaatca ccatggcagt aaatggatta
    16681 ccgccgaagc gtttccccgc ggtgtatccc aggattatcg ggaagaacca aaacaaggca
    16741 tcactggcgc tgaataaaat taaataagta ccactttgtt cggtcgtcca ctgaaaagtg
    16801 agcgccagag ccagcatacc tttcaggatc ccggttgccg ccatcaaacc gatcagaggc
    16861 gtaaaaatac ctgaaataac ataaacaaag cggtttagca gattaccttt ctcatcattt
    16921 tccggtgcct gttgcgcttt ttcgccaagg cctgccacgc tgttgaccgc caggaagaca
    16981 tcggccacat ggttacctat gaccacctga aactggccac cgctttccac caccataata
    17041 ataccggggg tgtttttcag tacctcagct tgcgctttgc tttcatcctt taacttaaaa
    17101 cgtaatcgcg ttgcgcaatg catcagacta acaatgttat ctgcgccccc gactcctgcg
    17161 actatttttc tggctaactc cgtcataact tgccctctac cgctttgcgg caaaactcca
    17221 aaaaaaacct gaaaaaaacg gcctgacgtg aatcaagcaa tttttttcag gttttgcccg
    17281 cttagtgcgg taacaatcct ttactcagta ataatatttc agtgttcttt gcgcacgcgc
    17341 tctatattta tggctaaaaa cataatctct gcgggtgaaa ttttacgttg atactgcaaa
    17401 ccaataaaaa tggcgatccg ttccgcacat tgccatgctt gcgggtaatt ttgttttact
    17461 gcttgttgta atgattcatc actatcgtta attgaagcat gttcaagaat acgccaggat
    17521 aaaaacttca gatgtgtaac cagtcgctga taactcaagc tttcttcctg gtaattaagg
    17581 ctgaactgaa attttattaa ttgcagcatt tcgcgcatta actgcgtgac acctgcaaca
    17641 tcctccatat ttccgctcat ttgggcactg accagatgca tggcaataaa gcccacttca
    17701 tctttcggta actgcacgcc caaccgttta tcaataatgg ttaacgcttc ttcccctagc
    17761 tggaactctt tcgggtaaag ccgctggata tcccacagca acgggttggg cagcaacacg
    17821 ttttgctgaa agcgtttaat cgcaaactgg caatggtcag ttagcgagat ataaatactg
    17881 tcctgtaatt ttcccaagcg ctcctgcgct aaagagataa tacgatcaca ggttgccatc
    17941 acctcaagag gaatatgact taagagttcg cttaatcgcc cgttcagttc atgactgctc
    18001 aaggcatact ctttttctat tccacttgag ttaattcttt cgccagcgcg tttttgaaag
    18061 ccaattcctc gccccatgac gactttttcc cgctgttgat catcaataac caccacaaca
    18121 ttattgttga gaattttggt gatttgcatg ttcatagcaa ggaccttttt tataaacaaa
    18181 aaaacccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga atgcagtaac
    18241 aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa ttaatttaat
    18301 tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct cgcagttatt
    18361 aactttgtgt aattttagga atttataaag ttatatataa caaatcccaa taattaagtt
    18421 attgggattt gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag tttatccagc
    18481 tcatcaccgc ctacatgacg gaaatcctgc cccttcacgt agtagaagat aaactcacaa
    18541 atattctggc agcggtcgcc aatacgttcg atagaacgcg cgcagaacag cgcagtaagt
    18601 acgctcggaa tggtacgcga atcttccatc atgtaggtca tcagttgacg aacaatacct
    18661 tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc gtcaatgtcc
    18721 atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg gcccagcgac
    18781 tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc agtacggcag
    18841 attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact gatcaccata
    18901 accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac gcaggcttca
    18961 tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg cttcgccaga
    19021 tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc caccatgccg
    19081 cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg gccggaaata
    19141 tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa cgaccggtga
    19201 tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg ttgctgaact
    19261 caatcaattc gcccaggtac ataaacgccg tgtggtcgga acaacgcgca gcctgctgca
    19321 tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc agctcttcaa
    19381 tacgcccggt agagataggg tcgagcgccg aacacggttc gtcgagcagc agcacttccg
    19441 ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca gagagagagt
    19501 aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg gtcaatgccc
    19561 actgcacgcg ctcgtccatg tcggcacggg agagcttctc aaacagacga acgccaaaag
    19621 cgatgttgtc gtagatggac atcggaaacg gcgtcggttt ctggaacacc atgcccactt
    19681 tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca tcaagcagaa
    19741 tttcaccttc cgcacgctgc tccgggtaca gttcaaacat tttgttgaag gtacgcagca
    19801 gcgtcgattt accgcagccg gacggcccga taaacgccgt tacctggttt ttagcgatat
    19861 ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa ttacgaacct
    19921 gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca tcgtgcctga
    19981 tgcacttcgc gaatcaggct taccgtaggc cggataaggc gttcacgccg catccggcaa
    20041 agtgttaacc gtgtttattc ttcgcaaaaa caacgcgcgc cagaatgttc agcagcagta
    20101 cgcacagggt aataatcaac accccggccc aggccagttg ctgccattct gcaaacgggc
    20161 tcatcgcaaa cttaaagatc gtcaccggca ggttggcgat cggctgcatc atgtccgtgc
    20221 tccagaactg gttggagagc gcggtaaaca gcagcggcgc ggtttcaccg gcaatacggg
    20281 caatcgccag caggataccg gtcatgatcc cggacaccga cgctttcagc gtaatcgcgg
    20341 agatcatttt ccacttcggt gtacccagcg cataagccgc ttcacgcagg ctgtccggca
    20401 ccagtttcag catgttctcg gtggtgcgga taacaatcgg cacctgcaac agcgccagcg
    20461 caattacacc cgcccagcca gagaagtgct ccatctgcgc caccacgatg gtgtaaacaa
    20521 acagaccaac cacaatcgac ggcgcggaga gcagaatgtc gttaatgaag cgaatcactt
    20581 ctgccagcca ggatttacga ccatattccg ccagataaat ccccgccata atgcccagcg
    20641 gcgtaccgaa taccgtggcc cacaaaatta acagcccgct acccgccagg gcgttcgcca
    20701 gaccaccacc ttccgtattg ggcggcggcg tcatttcagt gaacagcgcc agcgacatac
    20761 cgtcgatacc gcgagtgatg gtggacatta aaatccagat cagccagaac aggccgaagg
    20821 ccatcgtcgc catcgagagc gtcagcgcaa tacggttttt gaggcggcga cgcgcctgca
    20881 ttttgcggcg agattcagcc agcgccgcag tggtttgcat ttcaaccata gccattagcg
    20941 tgccccctca ttcttagcca ggcgcataat cataaacttc gatgcggcga ggacgatgaa
    21001 ggtaatcaca aacaggatca ggcccagttc catcagtgcg gcaacgtgca gaccggattc
    21061 cgcttccgca aattcgttcg ccagcgcaga ggtgatactg ttgcccggca tatacagcga
    21121 ggcgctgtcg agctggtagg tgttaccgat gataaaggtc accgccatgg tttcaccgag
    21181 cgcgcggccc agccccagca tgatgccgcc gataacacca tttttggtga acggaagaac
    21241 gatacgccag ataacttccc aggtggtgca gccaataccg taggccgact ctttcatcat
    21301 caccggggtt tgttcgaaca catcacgcat taccgccgca atgtacggaa taatcatgat
    21361 ggcgaggatc acgcctgccg cgaggatacc gataccaaat gcggggccag agaacagcgc
    21421 gccaacaatc gggatattcg acatgatatt gccgaccggc tcctgaaagt aaacggcgaa
    21481 cagcggcgca aagataaaca ggccccacat gccgtaaacg atacttggaa tggctgccag
    21541 cagctcaatg gcgataccca gcgggcgttt cagccagcca ggcgcaagct cagtcaggaa
    21601 cagggcgata ccgaaactca ccgggacggc gatcagcagc gcgataaacg aagtcaccaa
    21661 cgtaccgtag atcggcacca gcgccccgta gatatcgttc ggtgcatccc actctttggt
    21721 ccataggaaa gccagaccaa atttctgaat gctcggccag gaggagatga tcagagagac
    21781 aataatgcca cccaacatca atagcacaat cagcgccgcc agttttacca gcacgctgaa
    21841 aattatgtcg ccctttttac ccggtgggtt aaaagcaggc ttggttgcag ccataagtta
    21901 ctcttcagtt aaacgcgttt acaaagtcat tttgtaatgc cggatgcggc gtaaaacacc
    21961 gtacccggcc tggagtttta ttagtacagc ggcttaccgc tactgtcttt aatattggtc
    22021 ttccacgcag cgcgaacctg ttcaactacg ctatccggca ggctggcgta atccaggtcg
    22081 ttcgcctgtt tagccccggt tttgtaagcc cagtcgaaga acttcagcac ttctgtgcct
    22141 tgttctggtt tcttctgatc tttgtggatc agaatgaacg tggtagaggt aataggccat
    22201 gcatcttcgc ctttctggtt ggtcagatcc tgagcgaagg ttttgctcca gtctgcacct
    22261 tttgctgcat tagcgaagtt ttcttcggtc ggactaaccg gtttaccatc agcggagatc
    22321 agtttggtgt acgccaggtt gttctgcttc gcgtaagcat attcaacata accaattgca
    22381 cccggcagac gctgaacgaa cgcggcgata ccgtcgttac ctttaccgcc cagaccgatc
    22441 ggccatttta cggtagagcc agtaccaacg ttgtttttcc actcttcgtt cactttcgcc
    22501 aggtagctgg tgaagacgaa ggaagtcccg gagccatctg cgcggcgtac tacagcaatg
    22561 ttttgtgaag gcagtttcag acccggattc agtttggcga tggcttcatc atcccacttc
    22621 ttgattttgc ccaggtagat gtcgccgagg gttttaccat ccagcaccag ttcgccagac
    22681 ttcagccctg gaatgttaac cgccagcacc acgccgccaa tcacggtcgg gaactggaac
    22741 agaccttcct gagccagttt ttcgtcagac agcggcgcgt cagaggcacc aaaatcaacg
    22801 gtattagcga taatctgttt tacgccaccg gaagaaccga taccctggta gttaacttta
    22861 ttaccggttt ctttctggta agtgtcagcc catttggcat acaccggcgc agggaaggtt
    22921 gcacctgcac ctgtcaggct tgcttctgca aacacagaga aagcgctcat cgataaggtc
    22981 gcggcgacaa cagttgcgac ggtggtacgc ataactttca taatgtctcc tgggaggatt
    23041 cataaagcat tgtttgttgg ctacgagaag caaaatagga caaacaggtg acagttatat
    23101 gtaaggaata tgacattttt atgacagaaa ggaattacca aataacttac tgattttaaa
    23161 tgtgaagatg aaaatcccac atgtcatctt tttgtcatga tgcatttaaa agtgataaag
    23221 aaatgggtga gatgacaacg atgtgatgaa caatcgttgt cagacctatt agcgcacaat
    23281 ctcaacagtt atcgtcgctg ttgcagtaaa ttgccccggg gctgggcgag ctccagtaag
    23341 gctcgcagga gcagctgaaa aagttacact acctgatttt tcaccataca ctgttgattt
    23401 acccatgtct gttggcaact caccaccgtt cactttgatt tccttattat ttttgtcgta
    23461 aatttttatc cccacgttag agtttgaagt tacgaccatt gaattgtccg tattgggttc
    23521 ggtagcataa aaagacgtac tgatatcatc ggtattcagt atccccaggt tttcacactc
    23581 aatagtgacc gttttagtaa ttttatgtgt tgttaccgca cttccaacaa cagatgaaaa
    23641 atctgcagct ggtatttctt taaaatcaaa ataaactggc tgtaaattat ttatttcgca
    23701 actttgcgga gctgtaatat cgccccgaat ataaacatca gccatcggct gtgagtaatc
    23761 caccggggtg tcacggctaa ttgttccata caatgtcact atctttgttt caggaataat
    23821 caattttcct gcaaatgtct tcttgatata aaaatatact atcgctttag ccccactaga
    23881 tatacttaaa ggttcaatgc ggttgcaatt gtactttgta ttaccagacg ggttgttagc
    23941 atgatattca aaaggtgcat tcacataccc aaccccaaga atatctatag caatcccaac
    24001 atcaaggttc ggattaagat cataataatt aatattagag tgaaccccag cataagttaa
    24061 tgcctggttt atttttgacg tataatagat ataggggaat tctcctacgt taccagtatc
    24121 acaatgacag cgtccaggat atgaggatcc attagttaca gtggtgttaa atgtcgttcc
    24181 aaccttattt tcgttcgcgg taataagata tcccgagaaa tcaagcgtac tgtgaaatac
    24241 gccattatca ggggtacaga tacctttagg agctgcaagt gctacactac ttaacgagag
    24301 cataaacaca gcaaagcacc actgtttgat atacttgttc ataaaaatcc ttttaatgac
    24361 atgtcacacc ctgttcaatt aatccgctct cactttctgg tgtcgaaagt gaataattga
    24421 agatgcattt tgttgaggcg ttttctcccc aggataacgt aacctgtcct ttttctggta
    24481 gaccagaaag ataaagttcg ccacggtcac cgacaattcc tgaatctgcc aaacttgcat
    24541 cttgtatcgc agcaatggct ccaaaaggta ccggtttacc atttgcattt aacactctga
    24601 agagaacgcg ataacccaca tgagtcacaa actccgcttt aaccactgca ccacgcgtgg
    24661 gaaccatcgt aacgacggta ttatcaagtt ctatattcga cttcgagaaa tagttatcgc
    24721 tgattgctac ccgattttca tcataagggt ttagctgtgt cttgaccgta tagccgcgcc
    24781 agtccgtcgc agcaccgcgc atattatcta tttctaaccc ggcggcccca ggtgctttaa
    24841 caagagcaat agtttcacct aactcctgac ttaacgttac cccttcgctg tgtgccagaa
    24901 ttccccctct gataccataa ttcagcctct gggaattttg gctgtagtta tagctgccgt
    24961 tcaccgtgcc ataagagcca tcataggtca gggacatatt accgccaacc tcatcatcat
    25021 gactgttata ggactgcatt aaattccatg aaaggttatt tctttccagc gccgtaccgc
    25081 tgagtccgag ttgatgtgag ctaccggaat tatcgttttg cgaaacagaa taggtggcgt
    25141 aactattacc ccaccagccc gataaaggaa cagataaaga gagcatgtag atctgctcac
    25201 tggtgttatc actggcatgg ttgttccccg tcccctgtct ggcagtattg gaataactcc
    25261 aggagacgtt ccacgagaga cgcttccagc tactgctgta gccaaattgc attaatcgtt
    25321 cgacatcatc ggtatgccag tattgttgcc ggcttaatgt caggtataaa ttgccataat
    25381 ctctccccaa cgactgcgtt agcgttccct ccacgcgact acgtcggtta cctgtttccc
    25441 aaaagtcttc agggctgttg cgccgcgatg cccactcatt gagggtatag tagccctcgg
    25501 tggagtaacg atagccaacc aggcgcaagc tagtgtctgt gtcgtcaaat cgttttgaat
    25561 agagaaagcg atatgactga cccgtttctg tatcatcatg gctaaaattc gcacgagcat
    25621 gtgtcacatc gaaagaaatt gcgcccaaag cgccaagatc tttaccgata cccagagctg
    25681 cagaaccata attttcagca gcctgcatac ctccatataa agtgatcccg tgggaaaaac
    25741 catgaagtat ttgcgcctga aggacgtccg gtgtaaatcc gtcttcatcg cgcaattcac
    25801 ccacgctgac atcaacatct gtctgacctt cacgtttaag aatcgccaat gaagcataag
    25861 gttgggtgaa tcgttgttcg ctaccgtctt cttctttgat ggttacgtcc aaatcgccac
    25921 ctgtactggc ggtgttgaga tcggtgatct caaaagcacc ttgtggaacg gcagactgat
    25981 aaatcacgta gccattctgc cgaatgatga cggtggcgtt ggactttgca atcccacgaa
    26041 ccacaggagc aaagccattc tggctggcgg gtagcatatc gttatcagta tacaaacgcg
    26101 cgccgcgaat ttgcgtactg tcgaaaatat cgctcgccgt ccacgtatca ccaatcataa
    26161 tctggcttct taacgcggca atatcgcgct gtatccaggt actaatggag ttgtattccg
    26221 cacggccatc gctggtgttt aaagtactgt aattacgtag ccgccacggt ccaatattca
    26281 gtccattgcg cagattgaga taactggtat ctgtctcttg tgaaccataa tctgaacgta
    26341 gtgtatgtga accagaaaaa tcgtagttga tggtgagcgc gttaatcccc tcatcccaac
    26401 gcgtggggct gatgtaatca taagccatcg cgtccatgta gatttgggga atggatatat
    26461 tcagctgcaa tttatcactg tcaaactgcc aggatgagcc ttcaatacgt ggagcaggat
    26521 cgaggcaaac cgtctcatcc acatcattga acgatttgag cgcatcaact tttatgccaa
    26581 aggaggacag tgttttttta atggcaataa agcatgcgtg cagatcagca tctggattat
    26641 cctgaacaaa tgtcacatcc tgacgttcaa acattttacc gttgataaaa atatcaacgc
    26701 tataaacacc tgggggcatc aggtttgccc gattaaactg gcgaatatcg actccggatt
    26761 gatggtcgat ttccagaagc gccggattga atacactgac ctcggccatc aggttttgcg
    26821 agaaaatcag acacgtccct gccgtgaggc caaccactat tctggtcgtc atcataatac
    26881 gcctatcctt atcccttgct tacagcgtca tttttatcgg gtcactgact gcgccgtaat
    26941 cattaataaa tcgccaggtc atttggcctc ccgcagaatt agcaggtaaa ggtaacgtga
    27001 gcgtttcaaa aggacggatc atattgaggg gcacatcatg gccatcaacg atgacatcca
    27061 tcacactgat atgaattggg ttgggattct ctgctatcag atttcgccct tcccggcgcc
    27121 atttcacttt cggcgcagca tcttcgggaa taaggcggat atttgctggt cgccagaaca
    27181 gcttgatacg cgtttttact gctatttcca gcgagttcgt cgctttgttg ctcgctgatg
    27241 gcggaatatt tttaatattc agccagaaga ctgactcgcg atcatcaggc aatgacacac
    27301 ctttggtgtg aataatgcgt aatatttttt cttgcgaagg ttctaatcgt gatactggcg
    27361 gaataacggt aaacggcgct ttgcttttat cctctttatt aaatggatct acccaagcct
    27421 ggatgagata aggtattttt tcttctttgc taaatacaga aatagattta tcagcattat
    27481 tacttaagta aaccacccgc gtaccaccaa tgacaacgcc tgcataagtt aatgctggtt
    27541 ggcctaaaaa aacgctgagt ataatcgcgg taaataactt acatgaaact gttgttttaa
    27601 agagcctgat taaacaactt tttctggaca taacgactcc ctggccaaag ccaactgaat
    27661 aaaaaggccc ttgataaatt atttcttata ttcgacagta aattgtgaat cagcgttcgc
    27721 cgtaccagcc gttacggtcg cattggttgc cacatagcgt gcaataaact tcatttcagc
    27781 cttgccttca gcatcgatag cggtataagc agagtcgtta taaagtttaa cctgcgaatt
    27841 atcggactgg ttaaaaatac gaataccaac gccggttgcc gcaatcgctt gtcctgtacc
    27901 ggtaaaatca ccagggttgc ctgctgtaag cggcgtaccc actttcaggt cgccgtcgcc
    27961 gccaggcgct tctgtaccat caaaacgaac ggctgcttga gtgtacgttg ccgggcaatt
    28021 ttcaagctta atactgaact tctgtggcga cgccagacta cctacacctg aaaatgcatt
    28081 tttactgact ttacccagtg ggacaatttg atcggctgta tcggaggtaa cttcacaagt
    28141 agtgtcgaca atttcaccga cgaagtgaac aacaccatct tctgccagtg catgcccggc
    28201 cagcattaga gatgccagag tgaatgcacc gcctataata ttacgcttca taaaaactcc
    28261 attgatataa tatatccttt accatgaatc atcacttata ttaataagtg tatttttgat
    28321 tcaaaggaat agtacaccaa agagacggct ttatctaaaa ataatattaa ttacgcaaaa
    28381 ataaaatata aatttattgt ttaaatatta tttaaacagt aaaaaatcaa gtttgttatc
    28441 agacattaat gataattcag aatatattta tatagaaaga tagatgaatt tattgatgtc
    28501 agttataaaa aagggccacc ggtcggcagc ccttttttat ctatgatagt gattactcaa
    28561 ccgtaaccga ttttgccagg ttacgcggct ggtcaacgtc ggtgcctttg atcagcgcga
    28621 catggtaagc cagcagctgc agcggaacgg tgtagaagat cggtgcaatc acctcttcca
    28681 catgcggcat ctcgatgatg tgcatgttat cgctacttac aaaacccgca tcctgatcgg
    28741 cgaagacata caactgaccg ccacgcgcgc gaacttcttc aatgttggat ttcagttttt
    28801 ccaacagttc gttgttcggt gcaacgacga taaccggcat atccgcgtca atcagcgcca
    28861 gcgggccgtg tttcagctcg cctgcagcgt aggcttcagc gtgaatgtaa gagatctctt
    28921 tcagtttcaa tgcgccttcc agcgcgattg ggtactgatc gccacggccc aggaacagcg
    28981 cgtgatgttt gtcagagaaa tcttctgcca gagcttcaat gcgtttgtcc tgagacagca
    29041 tctgctcaat acggctcggc agcgcctgca gaccatgcac gatgtcatgt tcaatggagg
    29101 catccagacc tttcaggcga gacagcttcg ccaccagcat caacagcaca gttaactgag
    29161 tggtgaatgc tttagtggat gccacgccga tttccgtacc cgcgttggtc atcagcgcca
    29221 gatcggattc gcgcaccaga gaagaacccg gaacgttaca gattgccagc gaaccaaggt
    29281 aacccagctc tttcgacaga cgcaggcccg ccagggtatc cgcggtttcg ccagactgtg
    29341 acaaggttat catcaggctg ttacgacgca cggcagattt gcgatagcgg aattcagagg
    29401 cgatttcgac gtcgcacgga atacctgcca gcgattcaaa ccagtaacgg gaaaccatac
    29461 cggagttata agaagtacca caggcgagga tctgaatatg ctcaaccttc gacagcagtt
    29521 cgtcggcgtt cggtcccagc tcgcttaaat caacctgacc gtggctgatg cgcccggtaa
    29581 gggtgttttt tatcgcgttc ggctgttcgt agatctcttt ctgcatgtag tgacggtaaa
    29641 tacctttatc gcccgcgtca tattgcagat tggattcgat atcctgacgt tttacttccg
    29701 cgccagtttt atcgaagatg tttaccgaac ggcgagtaat ttccgcaata tcgccctctt
    29761 caaggaagat aaagcgacgg gtcaccggca acagcgccag ctggtcagaa gcgataaagt
    29821 tttcgcccat acccaggcca atcaccagcg gactaccaga acgtgccgcc agcagggtat
    29881 ccgggtgacg ggagtccatg atcactgtac cgtacgcacc acgcagttgc gggatagcac
    29941 gcagaacagc ctcacgcaga gtaccgccct gtttcagctc ccagttcacc agatgggcaa
    30001 ttacttcggt gtctgtttca gaaacgaagg tatagccacg cgcttttaac tcttcgcgca
    30061 gcggttcatg gttttcgatg atgccgttat gcaccaccac aatgtgttca gaaacatgcg
    30121 gatgcgcatt cacttctgaa ggttcaccgt gggtcgccca gcgagtatga gcaatacccg
    30181 taccgccatg cagaggatgt tcttccgctg cctgagccag catctggact ttaccgaggc
    30241 gacgcaggcg ggtcatatga ccttccgcat caacaacggc cagaccggca gagtcatatc
    30301 cgcggtattc cagacgacgt aaaccttcaa gaaggatttc tgctacatca cgttgcgcga
    30361 tcgcgccaac aattccacac atagtttttg attccgattt atatcgttgt cggtcaacct
    30421 gtatgcccgt tttttcgggc gccccgagcc ttgtagagag tggggttatt tttatagtta
    30481 ctgcttgtgg gagggagatt atgttatctc ctcatcccat gtgaccggat tacccggcca
    30541 gaatcacttt ttctttaccg gacgacgcca gccttctttc tgagtctgcg gcacacggct
    30601 gatagctaat gcattttcgc cgacattacg cgtcacagtt gtacccgcag caatggtcgc
    30661 gcctttgcct actgttaccg gggccaccag ctgagtgtcg gaaccgacaa acacatcgtc
    30721 gccgataatg gtcttaaatt tattcgcacc atcgtagttg caggtaatgg ttcccgcgcc
    30781 gatgttaacg ttatcgccaa tttccgcatc gcctaggtaa gtcagatgac cagctttcga
    30841 gcctttaccc agacgcgctt ttttcatctc aacaaagtta ccgacgtgtg caccttccag
    30901 caactcagca ccaggacgca gtcgggcaaa cgggccaata gtacaggccg ctgccagatt
    30961 cgcgtcttcc acaacggtat acggactgat ttcgcaatca tcgccaatca cgctgttttt
    31021 aatcacgcaa ccggtgccga ttttcacgcg atgaccgaga gtcacgttgc cctcgatgat
    31081 aacgttagta tcaatttcaa catcgcgccc gtgagtaagc gtaccgcgca aatcaaaacg
    31141 cgccggatcg cgcagcataa cgcctgctaa cagcagtttt tcagcctgtt cggactggta
    31201 aacgcgctcc agacgggaga gttgcaggcg gttattcaca ccttctactt cgcttaaacg
    31261 ttgcggatga acggcgacga tttcacgccc ttcctgatac gccagcgcaa taatgtcggt
    31321 gatgtagtat tcgccctgag cattattgtt ggtcagcttc gccagccagc gtttcatatc
    31381 tgcgccgtta gcgatcagaa tgccggtgtt gatctcctga atctgacgct gctcgtcggt
    31441 ggcatctttg tgctcaacaa tgccggtaac tttgccattt tcacgggtga tacgtccata
    31501 accggtcgga tcatccagtt tcaccgtcag cagaccaatg ccaccctgcg gtttagcatc
    31561 acgcaggcgc tggagtgttt cgacagagat cagcggcacg tcgccgtaga gcattaaaat
    31621 gtcttcatca tcggcaaaga aaggtgcggc ctgctgcatt gcatgacccg tacccagctg
    31681 ctctgcctga agcacccagt taaggttgtc gtctttcagc gcctgtttaa gcagatcgcc
    31741 gccgtgaccg tacaccaggt gaacgtgcgc tgcgcctaat tcattcgcag catcaatgac
    31801 atgctgaacc atcgctttcc cggcaagggt atgcagcact ttcggaagat cggaatacat
    31861 gcgcgtgcct ttgcctgcgg caaggatcac tacgctcata gcattattca acatacgcgt
    31921 cctgactgta atttgagaac gaatttaaac cgcttcacct tgaaaaaact acattttttt
    31981 catcgtgaaa tggacagagg ataaattgtt caataacgga ttatccccgc tgacgaaagt
    32041 gccattttcg accatcgtca cgccttttgt ctgcagaaaa ttaaggcagt accttcattt
    32101 aagcgtcagg tggatgtttt tgctcttatt tcgatcaatg aataaacggg aaaacagggg
    32161 tgttttatat cagcaggatc tatgtgaacg ctcttcagga cggatcacgc gtaaaaaaaa
    32221 gccagcctgt ttccagactg gcttttgtgc ttttcaagcc ggtgttacat cgcttttttg
    32281 gtcaactcga taacgcgcag ctgcgcgatc gctttggcca gttccgcaga cgcctgagcg
    32341 taatctacgt cgccgtgaga gctgctaatg tgctcttcag ccttacgttt cgcttccatg
    32401 gctcgcgctt cgtcgagatc ctggccgcga attgcggtgt cggccagaac ggtcacgttg
    32461 ccaggctgca cttcaagaat gccgccagac agatagataa actcttcgtg accgtgctgt
    32521 ttcacgatgc gaatcatacc aggcttaatg gcggtgagca gcggtgcgtg gccagggtag
    32581 atccccagtt caccttcgct acccgttacc tggattttct cgaccagacc agagaacatt
    32641 tgttgctctg cgctgacgac gtccaggtgg taagtcattg ccatatcacc ctccgattaa
    32701 ggcgttaaag ttttttggct ttttccacag cttcttcgat ggaaccgacc atgtagaacg
    32761 cctgctccgg caggtgatcg tattcgcctt ccatgatgcc tttaaagcca cggatggtgt
    32821 ctttcaggga gacgtattta cccggagaac cggtgaatac ttctgccacg aagaacggct
    32881 gggacaggaa gcgctggatc ttacgagcac gcgctaccac cagtttgtct tcttcagaca
    32941 gttcatccat acccaggatg gcgatgatgt ctttcagttc ctgataacgt tgcaggatgg
    33001 actgaacgcc acgcgcggtg tcgtagtgtt cctgaccaac caccagcggg tccagctgac
    33061 ggctggtgga gtccagcggg tcaacggccg ggtagatacc cagagacgcg atctgacggc
    33121 tcagtaccac ggttgcgtca aggtgcgcaa aggtggttgc cggagacggg tcagtcaagt
    33181 catccgcagg tacgtatact gcctgtacgg aggtgataga accagttttg gtggaggtga
    33241 tacgttcctg cagaacgccc atctcttccg ccagggtcgg ctgataacct accgctgaag
    33301 gcatacggcc cagcagtgcg gatacttccg tacctgccag ggtgtaacga tagatgttgt
    33361 caacgaacag cagaacgtca cgaccttcgt cacggaattt ctcagccatg gtcagaccgg
    33421 tcagagcaac gcgcagacgg tttcccggcg gctcgttcat ctggccatac accagggata
    33481 ctttgtcgat aacgttggag tcggtcattt cgtggtagaa gtcgttaccc tcacgagtac
    33541 gttcacctac gcccgcaaac acagagtaac cggagtgctc gatcgcgatg ttacgaatga
    33601 gctccatcat gtttacggtt ttacctacac ccgcaccacc gaacagacca actttaccgc
    33661 ccttagcgaa cggacacatc aggtcgataa ctttgatacc ggtttccagc agttcctgag
    33721 agtttgacag ctcttcgtag gaaggtgctg cgcggtgaat cgcccaacgc tcttcttcac
    33781 cgatctcgcc tttcatgtcg accggttcac ccagtacgtt catgatacgg cccagagtcg
    33841 ctttacctac cgggacttcg atcgggtgtt cgaggtcttt tacatccaga ccgcgacgca
    33901 gaccgtcgga ggaacccatt gcgatggtac gcacgatacc gccgccgagc tgctgctgaa
    33961 cttccagcac cagacgctca ttaccatttt gcacctcaag agcatcgtac acgcgcggta
    34021 cggcatcctg agggaattcg acgtcaacta cggcgccgat tacctggaca atctttccag
    34081 tagccatctt aaatcctcta cgaaataacc tgtttaaacc gcggcggccc ccgagacgat
    34141 ctcggtgagt tcctgagtaa tgctggcctg acgagctttg ttgtatacca actgcagctc
    34201 tttaatcagg ctgccgccat tgtcggtcgc ggctttcatc gccaccatac gggcggcctg
    34261 ctcgctggcc aggttttcaa ccacgccctg ataaacctga gattcgacat aacgacgcag
    34321 cagggtatcc agcaacgcct tcggatcggg ttcgtacagg taatcccagg atttatgttt
    34381 cagatcatca tcatctgatg ccggtaacgg cagcagctgg ctgatggtcg gaacctgaga
    34441 catggtgtta ataaatttgt tgctgacaat gtaaagcttg tccagacggc cttcgtcgta
    34501 ggcctgcaac atcactttta ccggaccgat cagttcggac agggaagggt tatcccccat
    34561 gccggtgacc tgggcaacaa cattgccgcc cacggagttg aagaacgaca cgcctttcga
    34621 gccgatcatt gcgaggtcgc attgaacgcc tttgtcggtc caggtcttca tttccgccag
    34681 cagttttttg aacaggttaa tgttcaaacc accgcacaaa ccacggtcgg tcgacaccac
    34741 caggtagccc acgcgtttaa cgtcgcgttc ttccaggtat gggtgcttat attccagatt
    34801 accgctcgca aggtgaccaa tcactttgcg catggtatct gcataaggac ggctggccgc
    34861 catgcgatcc tgcgatttac gcattttgga agcggcgacc atctccatcg ctttagtgat
    34921 cttttgcgtg ttctggacgc ttgcgatctt actacgtatc tcttttgcgc cggccatgag
    34981 cttctcctca atgccttgcg gcctgcccta aggcaagccg ccagacgtta ccaggattgg
    35041 gttgctttga aggaatcgag gatgcctttc agcttgcctt cgatttcgtc gttgtagcca
    35101 ccggtctggt tgatctcttg catcaacgga gcgtgatcac ggtcgacgta agccagcaga
    35161 gcggcttcga agctgccgat tttcgacagt tcaacatccg ccaggtaacc acgttctgct
    35221 gcgaacagaa ccagagactg ttgcgctacg gacatcggcg catactgttt ctgtttcagc
    35281 agttcggtca ctttctgacc gtggtcaagc tgcttacgtg ttgcatcgtc aaggtcggat
    35341 gcaaactgag agaacgctgc cagttcacga tactgtgcca gagcggtacg gataccaccg
    35401 gacagttttt tcatgatctt ggtctgtgct gcaccaccaa cacgggatac ggaaataccc
    35461 gggttaaccg caggacgaat accggcgttg aacaggttgg tttccaggaa gatctgacca
    35521 tcggtaatgg agattacgtt ggtcggaacg aacgcagaaa cgtcacccgc ctgagtttca
    35581 ataatcggca gtgcggtcag agaaccggtt ttccctttca cttcaccttt ggtgaaggct
    35641 tcaacgtatt cggcgttaac acgcgcagca cgctccagca gacgagagtg gaggtagaat
    35701 acgtcgcccg ggaatgcttc acgtcctggc ggacgacgga gcagcaggga gatctgacgg
    35761 taagcaacag cctgtttaga caggtcatcg taaatgatca gcgcatcttc accgcggtca
    35821 cggaagtatt cgcccattgc gcaaccggca tacggagcca gatattgcag tgcagcggat
    35881 tcagacgcgg ttgctaccac aacgatggtg ttagccagtg cgccgtgctc ttccagttta
    35941 cgtaccacgt tagaaatggt ggacgctttc tggccgatag cgacatagat acatttgata
    36001 ccggaatcgc gctggttgat gatggcatcg atagccagtg cggttttacc ggtctgacgg
    36061 tcaccgatga tcaattcacg ctgaccacga ccaattggga tcatggagtc aacggcttta
    36121 taaccggtct gtaccggctg atctacggac tgacgttcga taacgcccgg agcgattgct
    36181 tctacagcag agaagccgtc gtgatccagc ggacctttac cgtcgattgg tgcacccaga
    36241 gtgttaacca cacggcccag caggccacgg ccaaccggaa cttccaggat acggccagta
    36301 cacttaactt tcatgccttc ggcaaggtca gcgtacggac ccataacaac cgcacctaca
    36361 gagtcgcgct cgaggttcag tgcgatagcg taacggttac ccggcaggga gatcatttca
    36421 ccctgcatac aatcggccag gccgtgaatg cggataacac cgtcacttac agaaacaata
    36481 gtaccttcgt tgtgagcttc actcacaaca ttgaactgag caatgcgctg cttgatcagt
    36541 tcgctgattt cggtggaatt cagttgcatg ctccagtccc cttaagactg caagacgtct
    36601 gcaaggcgct caagacgacc gcgtacgctg ccatcaatga ccatatcacc cgctcggatg
    36661 ataacgcctg ccattacaga cttatcgatt ttgcaattca gcttaacttt gcgtgacaga
    36721 cgtttttcca tcgcagcaga aattttcgcg agctgttgtt cactcagtgc ggcagcggaa
    36781 atgacgtcta cctcagcggt agcctcactc acggcacgaa ggtgaataaa ctgctccaga
    36841 acatccggga gcgcgttaag acgaccattt tcagccatta cccgaatcag gttctgaccg
    36901 ttttcgtcca gttgctcacc acaaactgcg ataaacgact cggcgagcgt ttctggcgca
    36961 agcgcgccag agagaagctc tgccatttgt tcgtttttgg ttacctcggc ggcaaacgcc
    37021 agcatgtcct gccagcgttc tacactttgg tgttcgacgg caaagtcaaa agctgctttg
    37081 gcgtaggggc gagctaccgt aataaattca gacatcagcc cctccctcct tacagttcag
    37141 cgacaagttt atccacgatg tcgctgttag cagcttcatc cacggaacgt tcgatgatct
    37201 tctcggcgcc agcaacagcc aggatagcaa cttgcttacg cagctcttca cgggcacgtt
    37261 tacgctcggc ttcaatttct gcctgcgcct gggccacgat tttagtacgt tcctgttctg
    37321 cctcagcttt cgcttcgtcc agaatctgcg agcggcgttt gttcgcctgc tcgatgatta
    37381 cctgggcttc cgctttcgct tttttcagct ggtcggtcgc gctggccttt gcaaggtcaa
    37441 ggtccttatg tgctcgttct gcggaagcaa ggccgtcagc aatttctttt tgacgttttt
    37501 cgatggctgc cattaatggc ggccatacgt acttcatgca gaacagaacg aacaggacaa
    37561 acgcgatggc ctggccgagg attgttgcgt taagattcac agcacaatgc ctctatttag
    37621 ttaacgttct gatattgctc tttaaataaa agcaacgctt actacgcgac agcgaacatc
    37681 acgtacagac ccagacctac agcgatcatc gggatagcat ccaccagacc cataacgata
    37741 aagaactgag tacgcagcag aggaatcaga tcaggttgac gcgctgcgcc ttccaggaat
    37801 ttacccccga ggatgccgat accgatcgca gcaccgattg ccgccagacc catcatcaca
    37861 gcggcagcca tgtacagcag atccatattc aggttttcca tgacagtctc cagtttgttt
    37921 cagttaaaac gtagtagtgt tggtaaatta atgttcttca gacgccatcg acagatagac
    37981 gatcgtcaga accatgaaga tgaaggcttg cagcgtaatg atcaggatgt ggaaaatggc
    38041 ccacggcaca ttcaggatcc actgtgacca ccacggcaac agaccagcaa tcagaatgaa
    38101 aatcagctca ccggcataca tgttaccgaa cagtcgcaaa ccaagtgaaa ctggtttgga
    38161 cagcaggctt accccttcaa ggattaagtt gacaggaatg aacgcccagt gattgaacgg
    38221 ctgcagcgtc aactctttcg tgaagccgcc gatgcctttc attttgatgc tgtagaacag
    38281 aatcaggata aatacgccca atgccataga cagcgttacg ttcacgtccg cagacggaac
    38341 cacacgcagt gcaggcagac ccagtacatg ttcagcaatg tacggcagca ggtcgatagg
    38401 cagtaaatcc atcaggttca tcaggaatac ccagacgaag atcgtcaggg ccagcggagc
    38461 aatcagcttg cttttgccat ggtacatgtc tttcacgcta ccattaacaa agccgatcac
    38521 cagctcaatc gcggtctgaa acttacctgg cacaccgctg gtcgcctttt tggctacgct
    38581 acggaataaa accaggaaca acagacccag caccaccgag aagaacatgg agtcaatatt
    38641 gattgtccag aaggtggctg gggggttttg tggatccacc agcgagaatg tacgcaggtc
    38701 cagctgaagg ttattcaggt ggtgtcctat gtaatcctgc ggcgtcatat tttctgaagc
    38761 catgatgcct tttacccttt gttgttaatt acagccggtg ccagtatctg aaccaccagc
    38821 accaaaaccc acgtaacgat cagcggcaag aataccgcct ttaaaaccgc caacgccacc
    38881 accagtaaca ccaacatcgc cagaactttg aaagcttcgc caaatgcgaa tgtccaggcc
    38941 acccggcctt tcgctggtgt atgcgcctgg tgacgccagg caaatatcat aaacaaaacg
    39001 ttaggcagaa agactgccag gcccccgctt attgcagaga cgccccagaa ggggtctttg
    39061 aggctgaaca gcaatccact tgctatcacc accagtaact gaacgagcag aagcttccga
    39121 gcaacgtttc gactcacgag cgacacagac atcacgtttt tcactcctgc tcccttcgag
    39181 gtatgccgcg tgtcgtataa aactttcttt aaggcttaga gtcaagcatc aaaaagcggt
    39241 caaattatac ggtgcgcccc cgtgatttca aacaataagt agccaaaagg tgaataaatg
    39301 tttaaatatt tttccagtgc atacaattgc gacttttctg ctaaccctgt tcgatcatga
    39361 aaaactgtaa ataacgcgta aacactggtg ataaagcgtg cttcagatca catattgcgc
    39421 atgttcgcgc acagcatatt tatttacttg gcaaatgatg cctttgcaag tttatgatat
    39481 ttcagtctaa aaacagatac tgttttaata aatgacattt acacaacaaa aaccacccat
    39541 tgacattttt aataatgttt taacagccaa tgatggttct tagcgccgat ttttagcaga
    39601 ctgatatttt cactaatgac ttattttctg cttaccaaaa aaagccacgt tatcttgttg
    39661 atgcaaaaga gtgaacgtgg cgttaaatgt aaccagttat atcagtagaa aacctggttg
    39721 ttgttaacag tctaaccggt caatttttta tgattttttt gataaaaatt aaattttatt
    39781 tgctttaatc accaccagat gacgttcgcc atccagggct ggaacctgaa gtttaaccac
    39841 tgattcgacc tgatattctt cgggcaacaa agcgatttca tcttccggca tttgcccttt
    39901 cagcgcgtag aaacggcctt gctcaccagg aagatggtgg caccagctca ccatatcgtt
    39961 cagagaggca aaagcgcggc taattacgcc atcaaatggc ggctctgaag gaaactcttc
    40021 taccctgctc tgtactggtt caatattctc cagtttaagc tcatgttgca cctgacgaag
    40081 gaaacgcacg cgtttaccaa ggctatccaa cagagtgaaa tgggcttcag gacgcacgat
    40141 agagagtgga atgcctggca gtcctggtcc ggtgccgaca tcgataaacc gttcaccttg
    40201 cagatacggt gccaccacaa tgctatcgag aatatggcgt accagcatct cattaggatc
    40261 gcggaccgaa gtcaggttgt acgctttgtt ccatttatgc agcatattca cgtaggcaat
    40321 aagctggttt ttctggtgat cggtaagcga aatacctgcg tctttcagca gtaaggagag
    40381 tttgttgagc acggtgatta cctgttcttg atgcgttgcc tggtaagcgg gtgcttacca
    40441 ggcattttta atgcgttatg cgctacgacg cagcataccc tgttttttca gccacaccag
    40501 cagaatggag atggccgcag gcgtgacgcc agaaatacgc gaagcctggc cgatagaggc
    40561 tggtttgtga tcgttaagtt tggcgatcac ttcgttagaa agaccggata cctggcggta
    40621 atccagtgtc gcgggtagca gggtgttctc gttacgcagc tgcttttcga tctcatcttg
    40681 ctggcgcgcg atataacctt cgtatttaac ctgaatctca acctgttccg ccgcctgttc
    40741 gtctgtcaac gcaggggcaa acggcgtcag cgtggttaat ttttcataag tcatttccgg
    40801 acgacgcagc agatcttcac cactggcttc acgggaaagc ggcgcagtca ggtgagcatt
    40861 cacttcggct gcagcttccg ccgacggggt tacccaggtc gatttcagac gctgacgctc
    40921 acgctcgata ttctcaagtt tctcgttaaa gcgcgcccaa cgttcgtcat ccaccaggcc
    40981 cagttcacga ccgatttcag tcaaacgcag atccgcatta tcttcgcgta gcatcagacg
    41041 atattctgcg cgcgaagtaa acatacgata cggttctttg gttcctaaag tgcacaggtc
    41101 atcaactagt acgccgagat acgcctgaga acgtgccgga gcccaacctt ctttgtcagc
    41161 agacagacgg gcagcgttaa gaccggccag caaaccttgc gcagcggctt cttcgtaacc
    41221 ggtagtgccg ttaatctgac cagcaaagaa cagcccctgg ataaacttgc tctccagcgt
    41281 cggtttcagg tcgcgaggat cgaagaagtc atactcaatg gcataacccg gacgcacgat
    41341 cttcgcgttt tccatcccct gcatagagcg gacgatttgc atctgcacat cgaacggcag
    41401 gctggtggag ataccgttcg gataaatttc attagaggtc agtccttccg gttcaaggaa
    41461 gatctgatgc tgatttctgt cggcgaagcg cataactttg tcttcgatcg acgggcagta
    41521 gcgtgggccg acaccttcga tcacccctgc atacattggg ctacgatcga ggttactgcg
    41581 gatcacatca tgggttttct cgttggtatg agtgatataa cacggcacct gctggggatg
    41641 ctgggacgca ttgcccataa acgagaatac cggcattggg ttatcgccat gctgttgcgc
    41701 cagcacacta aagtcgatag ttcgagcatc aatacgcggt ggtgtcccgg ttttcagacg
    41761 accaacgcgc agcggcagtt cacgcaaacg gcgagaaagc ggaatggacg gcggatcacc
    41821 agcacggcca ccgctgtaat tatccagacc gatatgaatt ttaccgtcga ggaacgtccc
    41881 aacggtgagc acgacggctt tggcacggaa cttcagtccc atttgggtaa cagcaccgac
    41941 cacgcgatcg ttttcgacaa taagatcttc aaccgcctgc tggaagatca tcaggttcgg
    42001 ttggttctcc agcgccgtac gtaccgcctg acggtagagc acacgatccg cctgagctcg
    42061 ggtagcgcga accgccggtc ctttgcttgc gtttagtatc ctaaactgga tacccgcttg
    42121 atcgatcgct ttcgccatca gaccgccgag tgcatccact tcttttacca gatgtccctt
    42181 cccaataccg ccgatcgccg ggttgcagct catctgcccc agagtgtcga tattgtgtgt
    42241 caaaagcaga gtctgttgac ccatacgcgc cgcggccatc gcggcctcgg tgcctgcatg
    42301 acccccgcca atgatgatga cgtcaaaagg atccggataa aacatggtga ttgcctcgca
    42361 taacgcggta tgaaaatgga ttgaagcccg ggccgtggat tctactcaac tttgtcggct
    42421 tgagaaagac ctgggatcct gggtattaaa aagaagatct atttatttag agatctgttc
    42481 tattgtgatc tcttattagg atcgcactcc ctgtggataa caaggatccg gcttttaaga
    42541 tcaacaacct ggaaaggatc attaactgtg aatgatcggt gatcctggac cgtataagct
    42601 gggatcagaa tgaggggtta tacacaactc aaaaactgaa caaccgttgt tccttggata
    42661 actaccggtt gatccaagct tcctgacaga gttatccaca gtagatcgca cgatctgtat
    42721 acttatttga gtaaattaat ccacgatccc agccattctt ctgccggatc ttccggaatg
    42781 tcgtgatcaa gaatgttgat cttcagtgtt tcgcctgtct gttttgcacc ggaatttttg
    42841 agttctgcct cgagtttatc gatagcccca caaaaggtgt catattcacg actaccaata
    42901 ccgattgcgc caaagcggac cgcagaaaga tcgggcttct gttcctgcaa tgcttcatag
    42961 aaaggagaaa ggttgtccgg aatatctccg gcaccgtggg tggagctgat aatcagccag
    43021 atccccgagg cagataaatc ttccaacagc ggaccgtgca gcgtttcggt ggtaaaaccc
    43081 gcctcttcca gcttttcagc caggtgttct gctacatatt cggcaccgcc gagggtgctg
    43141 ccgctgataa gagtgatatc tgccataaac cgccaccttt attaagagtg gcgtattgta
    43201 cgctgtgaac gcgttgggat ctacctgtgg aaaagtatgg gattaaaaaa gccgattatg
    43261 gcttgatggt acgcatgatc gggttttgca ggacgatcaa tgtctcggtg gactgaattt
    43321 catcaattgt ttggatcttg ttgataagta catgctggag agcgtcgatc gaacggcaca
    43381 tcacttttat aaagatgctg tagtggccgg ttgtgtaata ggcttcagtg acttcatcaa
    43441 ggctttccag ctttgccagc gcggaagggt agtctttggc gctctttaat ataatgccga
    43501 taaagcagcc tacgtcataa ccgagctgct tcgggctgac atcaatacgc gccccggtaa
    43561 tgatccccgc ctgcttcatt ttctctactc gaacgtgaat cgtccccgga ctgacgccaa
    43621 attgtttcgc cagttcggcg taagcggtgc gcgcattgcc cattaatgct tccaggatgc
    43681 cacggtccag attgtcgatc agataatttt ccataggatt ttcttatgca gattgatgat
    43741 tcattctatt ttagccttct tttttaatga atcaaaagtg tgttaggctt tttattgaat
    43801 gattattgca tgtgtgtcgg tttttgttgc ttaatcataa gcaacaggac gcaggagtat
    43861 aaaaaatgaa aaccgcttac attgccaaac aacgccaaat tagcttcgtg aaatctcact
    43921 tttctcgcca actggaagaa cgtctggggc tgatcgaagt ccaggcgccg attcttagcc
    43981 gtgtggggga tggcacgcag gataacttgt cgggctgtga aaaagcggtg caggtaaaag
    44041 tgaaagctct gcctgatgcc cagttcgaag tggttcattc actggcgaag tggaaacgtc
    44101 agaccttagg gcaacacgac ttcagcgcgg gcgaagggct gtacacgcac atgaaagccc
    44161 ttcgccccga tgaagagcgt ctttctccgt tgcactcggt ctatgttgac cagtgggact
    44221 gggaacgcgt aatgggcgac ggtgagcgtc aattctcgac tctgaaaagc acggtagagg
    44281 cgatctgggc gggaattaaa gcaaccgaag ctgcagttag cgaagagttt ggtttggcac
    44341 cgttcctgcc ggatcagatc cacttcgtac acagccagga gttactgtct cgttatccgg
    44401 atctagatgc caaagggcgt gagcgggcga ttgcgaaaga tcttggcgcg gtattcctcg
    44461 tcgggattgg cggcaagctg agtgatggtc atcgtcacga cgtgcgcgca ccggattatg
    44521 atgactggag caccccgtca gagctgggcc atgcgggtct gaacggcgat attctggtat
    44581 ggaaccctgt actggaagat gcgtttgagc tttcttccat ggggatccgc gtggatgccg
    44641 acacgttgaa gcatcagttg gcgctgaccg gtgatgaaga tcgtctgcag ctggaatggc
    44701 atcaggcgct gctgcgcggt gaaatgccgc agaccatcgg cggcggtatc ggccagtctc
    44761 gtttgaccat gctgctgctg caactgccgc atatcggcca ggttcagtgt ggagtatggc
    44821 cagctgctgt tcgcgagagc gtcccttctc tgctgtaata atttatcgcc gccagcgtct
    44881 gagcaggcgg cttcgcatcc cggtatcaaa gcgccagata tgatcgaaaa tgcgcatgat
    44941 gccgggtttg ccgtgtgccg acatcgccac ggcatgaaag cgatgctgat gtacccgctg
    45001 cagctctttc actttactcg tcacgtcgtc aggcaaccgc tgagcgataa aatcagaaat
    45061 caccaccgca tcggcatcaa accattccct gctttgcaag cgttccataa tggcgcgaaa
    45121 acaactggca agatcggtgc cgccacgaaa ctgctggctt aaaaaacgga ttgcttgttc
    45181 gatgccttgt gggcctgaaa gctcataacg gacgatctcg gtggaaaata gcataatata
    45241 gcagcgccgg ttttcagcga gagcaatgcg catcaaggcc aggcagaacg ctttcgcaca
    45301 ctgttcatta aagccgccca ttgagccgga agtatccaca cagacaataa acggcccgcg
    45361 cggctgttca tcgtaatctt tatgtaccac cggacgttcg atcacttttt cacgccacga
    45421 ctcaccgtgc aggcgatagg tgagcaactg tttttccacc agccgacggt aaaactcata
    45481 ctccagttcc gttatcccta gtgtcgccag ttctggcggc agaagacgta aaatatcatc
    45541 gctttgttgc agaccatcaa cctgctcagg aaccgtcgcc ggttcgcgca ccatggtgcg
    45601 gaaggtttcc atctgcgcat cgttgcgcgg tattgatttg gcttcccgag aacgccccag
    45661 ctgctctgcc aggcgtttca gttccggctg ttcgttaaga aattcaccgt atttcacaat
    45721 caactgatag tcgccacgtt taagctgacc ggcgctcata tcccacagac gaccagctgc
    45781 agtattgtta tctgcgagaa tcggttcaag ttgtccgctc agcgtcatgc gttcctgaac
    45841 ttcactcaac agttgttcgc gttcttcttc taatagctgt tgatttaacg tcgttgcttg
    45901 cacgatcaga cttaaacgcc aacgctggag aaaaagcgtg tgtaacgctg aagtgatcgt
    45961 gctgttagca tcaaccaact gtcgggcttg ttctgcccat ggagaattca gacgatgcag
    46021 taagtccagg atctgtggta gctgcacaat aaactgtggc gtggagagga gctggctttg
    46081 ctgatagcac atcacctctt cggtgagttc cggcgggact cgggcatctt tcagccgact
    46141 gcgcagcgct tcacgccagc ggggaacatc atcagtgatt gccgccttca ggcgtgggaa
    46201 tttttcaaag aagaccgcca gctgcggtga ggccagcagt gcgatgatca tctcttcgat
    46261 caatccctct tcgctgacgg ccagcatcac attaagcgta tccagcgtta gcattgttgt
    46321 gcctggcgaa tctgtgcacc gacatcctgt aggctggctt caatgcgacc taaccagtcg
    46381 ccagggataa acagacattt ttgctgttcg ctgaataaag cgtgttgctt gcgccagtcg
    46441 ctttcaagct cttccagttg ttgttttatt tcaccaggca gaccttcagc cgatgaaccg
    46501 ggaagtgcca gcgtactgcc ttgtaaactt acatcgcgaa cgacaagatg ttgggcgcta
    46561 tcaacttcca gattcagttt ctgggcaaag ccgataccgt tcagtttacc gcgaatttca
    46621 ccacctttgc tcagccactg ttccagcgcg ctacgctcaa aggagatatg aaccacttcc
    46681 atatcatgca gttttaacgg tttttgcagc agtagagtca gagtggaagc agtaacgtta
    46741 acaggaagtt gatactgctg acgacggctg aaaatgccgc ccagacgaat taccgttaag
    46801 gctgttttat cgctttgttg ctgctggagt tgcaggtgac gttgcacaat cgcgccaagg
    46861 cgagtcaaca tcccttgctg ttgccaggcg tgaccggtca tcaatacatc aatttgttgt
    46921 tgtatcaaat tcaggctttg cgcgtcatac cacaggcaat ctttcagcaa aatgagatct
    46981 accggggcaa cagcactgcg accgctaaaa aaggcgctgg cctgcaataa tcggatcgct
    47041 tttttccagc gacgatccga gacataaggc gcatccggta atttatccag ttgctggcgc
    47101 agcataaaaa tcagctcaaa tacatgatcg ggcagcgtaa tttcaccaat ctctttctgc
    47161 cagcgttcat attcttcatc tgtgacctgc aaggcatcag gaaccggatt gtcgttttca
    47221 tcctgttgac tggtcagcat ggagcggaaa ttcgctttat cctgcacttt atctaaccac
    47281 agacgaatca gcatgcggtc atataacgct tccagactgc tgtctgcttc cggcagctcg
    47341 ttggaggccg ccaccagcag acgcatcggg attttttcta cgtgtgcacc gttgcggaac
    47401 tggcgctcgt taatggcggt gagcaaggta ttaagaattg ccgggcccgc tttccagatc
    47461 tcatccagaa agacaatttc tgcttccggc aggtaaccgc tggttaaacg ttcatagcgc
    47521 ccttcatctt ttagcgcctg aatagaaagg ggaccaaaaa cttcttccgg cgtggagaag
    47581 cgggtcatca gatattcaaa cgcgcgggca ttctgaaagg cgaattttaa gcgccgggcg
    47641 atcaaacttt tggcaatacc tggcgggcca aggaggaaca cactttcacc acttaatgcc
    47701 gctaataaac acaagcggat ggcgtggcta cgttcataaa gccccttttc caacgaactg
    47761 ctcaggcggg aaattctttc cgctaataaa tgagggtgag ccataatgaa gtggcgtcct
    47821 ttcgtcaaaa gttctgcgta aattgcgagt atagacgttt cttgctggtg gctaaaatag
    47881 tctcaaaggg ggggtatttt tctttgagcc aggttaatgt ggccgcattt aggagtacga
    47941 ttttgccgtt aatcgtgcat actgtgcgct tttttgtggg ccaagggact aagcacacat
    48001 ttcatatttc aacgaaagac tagtctatga gcactgataa taagcaatca ttgcccgcga
    48061 ttaccctcgc ggcgattgga gttgtctacg gcgatattgg taccagcccg ttatatacac
    48121 ttcgtgaatg tttgtccggc cagtttggtt ttggcgttga acgcgatgcc gtgtttggct
    48181 ttttatcgct gatcttctgg ctgctaatct ttgtggtttc cattaaatat ctcaccttcg
    48241 tgatgcgggc agataacgcc ggtgaggggg ggatcctgac gttgatgtcg cttgccgggc
    48301 gtaatacgtc ggcgcgaacc acatcaatgc tggtgattat ggggctaatc ggcggcagct
    48361 ttttctatgg tgaagtcgtc ataacacccg ctatttcggt gatgtcagcc attgaaggtc
    48421 tggaaatcgt cgccccgcag ctggatacct ggatagttcc cctctcaatt atcgttctca
    48481 ctttattatt tatgattcaa aaacatggca cggctatggt cggtaagctg tttgcgccga
    48541 tcatgctgac ctggtttttg attctggcag gactggggtt acgtagcatt attgctaacc
    48601 cggaagtgct gcatgcgctg aatccaatgt gggcggtgca tttcttcctt gaatacaaaa
    48661 cggtttcttt tattgcatta ggggcagtgg tgttgtcgat tacgggggtc gaggcgctgt
    48721 atgctgatat ggggcacttt ggtaagttcc ctattcgcct ggcgtggttc accgtcgtat
    48781 taccttcctt gacccttaat tacttcggtc agggagcgct gttgttaaag aacccggaag
    48841 cgattaagaa cccgttcttc ctgttggcac cggactgggc gctgatcccg ctgctgatca
    48901 tcgccgcact ggcgacggta attgcctcgc aggcggttat ctctggcgtc ttctcattga
    48961 cgcgtcaggc ggtacgtctg ggatatttat cgccgatgcg cattattcac acctccgaaa
    49021 tggagtcagg gcaaatctat attccgtttg tgaactggat gctctatgtc gcggtcgtga
    49081 ttgtgattgt cagctttgag cactccagca acctggcggc ggcgtacggg attgcggtga
    49141 ccggaaccat ggtgctgacg tctattctct cgactaccgt ggcacgtcag aactggcact
    49201 ggaataagta ttttgttgcg ctgatcctga ttgctttcct ttgtgtcgat attccattgt
    49261 tcaccgctaa cctcgataaa ctgctctccg gcggctggtt gccattgagc ctcggtacgg
    49321 tgatgtttat cgtgatgacc acctggaaga gcgagcgttt ccgcttgctg cggcggatgc
    49381 atgaacatgg taactctctg gaagcgatga ttgcttcgct ggagaaatca ccgcctgttc
    49441 gcgtgcccgg gaccgcggtg tatatgtcgc gtgcaatcaa cgtcattccc tttgcgctga
    49501 tgcataacct taaacataac aaggtattgc atgagcgggt gattctgtta actctgcgta
    49561 ccgaagacgc gccatatgtc cataatgtcc gtcgggtaca gattgaacaa ctgtcgccca
    49621 ctttctggcg cgtggtggca agttatggtt ggcgagaaac gccaaacgtg gaagaagttt
    49681 tccaccgctg cggtctggaa ggattaagtt gccggatgat ggaaacctcc ttctttatgt
    49741 cgcatgagtc gttgatcctc ggcaaacgcc cgtggtattt gcgtctgcgc ggcaagctgt
    49801 acttgctgct gcaacgtaat gcgctgcgtg caccagatca atttgaaatt ccgccaaaca
    49861 gggttatcga actgggtact caggtcgaaa tctaacgcca gacgcctcct ttctccataa
    49921 gggggcgttt ttgttttcat ggttaatcac catgtaaaac gtttcgaggt tgatcacatt
    49981 tccgtaacgt cacgatggtt ttcccaactc agtcaggatt aaactgcggg tcagcgaaac
    50041 gtttcgctga tggagaaaaa aatgaaaaaa ggcaccgttc ttaattctga tatttcatcg
    50101 atgatctccc gtctgggaca taccgatacg ctggtggtgt gtgatgctgg tttacccatc
    50161 cccaaaagta caacgcgtat cgatatggca ttaacccagg gtgtaccttc ttttatgcag
    50221 gtgctgggcg tcgtcacaaa tgaaatgcag gtcgaggctg ccattatcgc ggaagagatc
    50281 aaacaacata atccgcaact ccacgaaacg ttgctcactc accttgagca gctgcaaaaa
    50341 caccagggaa ataccattga aattcgttac accacgcatg aacaattcaa acaacaaacc
    50401 gcagaaagtc aggcggtaat tcgcagcgga gaatgttctc cgtatgcgaa tatcattctc
    50461 tgtgctggcg tgacgttctg aggccgtcat ggaagcatta cttcagctta aaggcatcga
    50521 taaagccttc ccgggcgtaa aagccctctc gggcgcagcg ttaaatgtct atccgggccg
    50581 cgtgatggcg ctggtgggcg aaaacggcgc gggtaaatcc accatgatga aagtgcttac
    50641 tggtatctat gctcgcgatg ccggcacgct tttatggctg gggaaagaaa cgacatttac
    50701 cgggccgaaa tcttcccagg aagccgggat tgggattatc catcaggaac tgaacctgat
    50761 cccgcagttg accattgccg aaaacatttt cctcggtcgt gagtttgtta atcgctttgg
    50821 caaaattgac tggaaaacca tgtatgccga agcggataaa ttgctggcta aacttaacct
    50881 gcgctttaaa agcgacaagc tggtgggcga tctttccatc ggtgaccagc aaatggttga
    50941 aatcgccaaa gtgctgagct ttgagtcgaa agtcatcatt atggatgaac cgaccgatgc
    51001 gctgaccgat accgaaaccg aatccctgtt ccgcgtcata cgcgagctga aatcgcaagg
    51061 ccgcggtatt gtctatatct cccaccgcat gaaagaaatc ttcgagattt gcgatgacgt
    51121 taccgttttt cgtgacgggc aatttattgc tgagcgcgaa gtggcatcac tgaccgaaga
    51181 ttcgctgatt gagatgatgg tgggccgcaa gctagaagat caatatccac acctggacaa
    51241 agcgccggga gatatccgcc tgaaagtcga taatctctgc ggacctggcg ttaacgatgt
    51301 ctcttttact ttacgcaaag gcgaaattct tggcgtctct ggtttgatgg gcgcaggtcg
    51361 taccgaactg atgaaagtgc tctacggcgc actaccgcgc accagcggtt acgtcaccct
    51421 ggatgggcat gaagtcgtta cccgttcacc gcaggatggc ctggcaagcg gcattgtgta
    51481 tatctccgaa gaccgtaaac gtgacggttt agtgttgggc atgtcagtaa aagagaacat
    51541 gtcgctgaca gcgctgcgct acttcagccg cgctggcggc agtttgaagc atgccgatga
    51601 acagcaggct gtgagtgatt tcattcgtct gtttaatgtg aaaacaccgt cgatggaaca
    51661 ggcaattggt ctgctttccg gtggcaatca gcaaaaagtg gcgattgccc gcggtctgat
    51721 gacacgcccc aaagtgttga tccttgatga acctacccgt ggcgtagatg tcggcgcgaa
    51781 aaaagagatc tatcaactga ttaaccagtt caaagccgat ggcttgagca tcattctggt
    51841 gtcatcggag atgccagaag tattaggcat gagcgatcgc atcatcgtca tgcatgaagg
    51901 gcatctcagc ggggaattta ctcgtgagca ggccacccag gaagtgttaa tggctgccgc
    51961 tgtgggcaag cttaatcgcg tgaatcagga gtaaaaaaat gacaacccag actgtctctg
    52021 gtcgccgtta tttcacgaaa gcgtggctga tggagcagaa atcgcttatc gctctgctgg
    52081 tgctgatcgc gattgtctcg acgttaagcc cgaacttttt caccatcaat aacttattca
    52141 atattctcca gcaaacctcg gtgaacgcca ttatggcggt cgggatgacg ctggtgatcc
    52201 tgacgtcggg catcgacttg tctgtaggtt ctctgttggc gctaaccggc gcagttgctg
    52261 catctatcgt cggcattgaa gtcaatgcgc tggtggctgt cgctgccgct ctcgcgttag
    52321 gtgccgcaat tggtgcggta accggggtga ttgtagcgaa aggtcgcgtt caggcgttta
    52381 tcgctacgct ggttatgatg cttttactgc gcggcgtgac catggtttat accaacggta
    52441 gcccagtgaa taccggcttt actgagaacg ccgatctgtt tggctggttt ggtattggtc
    52501 gtccgctggg cgtaccgacg ccagtctgga tcatggggat tgtcttcctc gcggcctggt
    52561 acatgctgca tcacacgcgt ctggggcgtt acatctacgc gctgggcggc aacgaagcgg
    52621 caacgcgtct ttctggtatc aacgtcaata aaatcaaaat catcgtctat tctctttgtg
    52681 gtctgctggc atcgctggcc gggatcattg aagtggcgcg tctctcctcc gcacaaccca
    52741 cggcggggac tggctatgag ctggatgcta ttgctgcggt ggttctgggc ggtacgagtc
    52801 tggcgggcgg aaaaggtcgc attgttggga cgttgatcgg cgcattaatt cttggcttcc
    52861 ttaataatgg attgaatttg ttaggtgttt cctcctatta ccagatgatc gtcaaagcgg
    52921 tggtgatttt gctggcggtg ctggtagaca acaaaaagca gtaataacgt ctacaggaca
    52981 tcttgaatat gaacatgaaa aaactggcta ccctggtttc cgctgttgcg ctaagcgcca
    53041 ccgtcagtgc gaatgcgatg gcaaaagaca ccatcgcgct ggtggtctcc acgcttaaca
    53101 acccgttctt tgtatcgctg aaagatggcg cgcagaaaga ggcggataag cttggctata
    53161 acctggtggt gctggactcc cagaacaacc cggcgaaaga gctggcgaac gtgcaggact
    53221 taaccgttcg cggcaccaaa attctgctga ttaacccgac cgactccgac gcagtaggta
    53281 atgctgtgaa gatggctaac caggcgaaca tcccggttat cactcttgac cgccaggcaa
    53341 cgaaaggtga agtggtgagc cacattgctt ctgataacgt actgggtggc aaaatcgccg
    53401 gtgattacat cgcgaagaaa gcgggtgaag gtgccaaagt tatcgagctg caaggcattg
    53461 ctggtacatc cgcagcccgt gaacgtggcg aaggcttcca gcaggccgtt gctgctcaca
    53521 agtttaatgt tcttgccagc cagccagcag attttgatcg cactaaaggt ttgaacgtaa
    53581 tgcagaacct gttgaccgct catccggatg ttcaggctgt attcgcgcag aatgatgaaa
    53641 tggcgctggg cgcgctgcgc gcactgcaaa ctgccggtaa atcggatgtg atggtcgtcg
    53701 gatttgacgg tacaccggat ggcgaaaaag cggtgaatga tggcaaacta gcagcgacta
    53761 tcgctcagct acccgatcag attggcgcga aaggcgtcga aaccgcagat aaagtgctga
    53821 aaggcgagaa agttcaggct aagtatccgg ttgatctgaa actggttgtt aagcagtagt
    53881 tttaatcagg ttgtatgacc tgatggtgac ataaatacgt catcgacaga tgaacgtgta
    53941 atataaagaa aagcagggca cgcgccaccc taacacggtg gcgcatttta tggacatccc
    54001 gaatatgcaa aacgcaggca gcctcgttgt tcttggcagc attaatgctg accacattct
    54061 taatcttcaa tcttttccta ctccaggcga aaccgtaacc ggtaaccact atcaggttgc
    54121 atttggcggc aaaggcgcga atcaggctgt ggctgctggg cgtagcggtg cgaatatcgc
    54181 gtttattgcc tgtacgggtg atgacagcat tggtgagagc gttcgccagc agctcgccac
    54241 tgataacatt gatattactc cggtcagcgt gatcaaaggc gaatcaacag gtgtggcgct
    54301 gatttttgtt aatggcgaag gtgagaatgt catcggtatt catgccggcg ctaatgctgc
    54361 cctttccccg gcgctggtgg aagcgcaacg tgagcgtatt gccaacgcgt cagcattatt
    54421 aatgcagctg gaatcaccac tcgaaagtgt gatggcagcg gcgaaaatcg cccatcaaaa
    54481 taagactatc gttgcgctta acccggctcc ggctcgcgaa cttcctgacg aactgctggc
    54541 gctggtggac attattacgc caaacgaaac ggaagcagaa aagctcaccg gtattcgtgt
    54601 tgaaaatgat gaagatgcag cgaaggcggc gcaggtactg catgaaaaag gtatccgtac
    54661 tgtactgatt actttaggaa gtcgtggtgt atgggctagc gtgaatggtg aaggtcagcg
    54721 cgttcctgga ttccgggtgc aggctgtcga taccattgct gccggagata cctttaacgg
    54781 cgcgttaatc acggcgttgc tggaagaaaa accattgcca gaggcgattc ggtttgccca
    54841 tgctgccgct gcgattgccg taacacgtaa aggcgcacaa ccttccgtac cgtggcgtga
    54901 agagatcgac gcatttttag acaggcagag gtgacgcttg gctacaatga aagatgttgc
    54961 ccgcctggcg ggcgtttcta cctcaacagt ttctcacgtt atcaataaag atcgcttcgt
    55021 cagtgaagcg attaccgcca aagttgaagc ggcgattaaa gaactcaatt acgcgccatc
    55081 agccctggcg cgtagcctca aactcaatca aacacatacc attggcatgt tgatcactgc
    55141 cagtaccaat cctttctatt cagaactggt gcgtggcgtt gaacgcagct gcttcgaacg
    55201 cggttatagt ctcgtccttt gcaataccga aggagatgaa cagcggatga atcgcaatct
    55261 ggaaacgctg atgcaaaaac gcgttgatgg cttgctgtta ctgtgcaccg aaacgcatca
    55321 accttcgcgt gaaatcatgc aacgttatcc gacagtgcct actgtgatga tggactgggc
    55381 tccgttcgat ggcgacagcg atcttattca ggataactcg ttgctgggcg gagacttagc
    55441 aacgcaatat ctgatcgata aaggtcatac ccgtatcgcc tgtattaccg gtccgctgga
    55501 taaaactccg gcgcgcctgc ggttggaagg ttatcgggcg gcgatgaaac gtgcgggtct
    55561 caacattcct gatggctatg aagtcactgg tgattttgaa tttaacggcg ggtttgacgc
    55621 catgcgccaa ctgttatcac atccgctgcg tcctcaggcc gtctttaccg gaaatgacgc
    55681 tatggctgtt ggcgtttacc aggcgttata tcaggcagag ttacaggttc cgcaggatat
    55741 cgcggtgatt ggctatgacg atatcgaact ggcaagcttt atgacgccac cattaaccac
    55801 tatccaccaa ccgaaagatg aactggggga gctggcgatt gatgtactca tccatcggat
    55861 aacccagccg acccttcagc aacaacgatt acaacttact ccgattctga tggaacgcgg
    55921 ttcggcttag atttacgctg tcttttgatc aaattattac catcggttgt tttcagaagc
    55981 atgaacattg ctgctgaagc aacagtaata atgcccatcg tgataaacgt atagtggaat
    56041 tgttcgacag tcgttgtgcc ttccatccct tcataaacgc gaaggacggc cgcacttaca
    56101 gcaacgccta aactaatcga cagttgctgc gtgaccgcca gaacactgtt accgctgctg
    56161 gcgttgtcat cggtcagatc ggtaagtgtg atggtattca tcgcggtaaa ttgcgtcgac
    56221 atagccatcc ctaatataaa caacggcaag atcagcatcc atatcgccat tgccggtgat
    56281 tgcaaagaga actgagcgat cattagccca ataatcaccg tgatccccac taacgtatgg
    56341 cgatagccca gacgacgtaa gacttgggta accatcgatt ttgcaataat ggaacctaac
    56401 gctgtcggtg ccatcataca gccggcaata aacgcctgat aaccaaatcc tacctgtaac
    56461 atcaatggca taaggaacgg tacacaaccg gtccccagac gggttgcaat attgcctacg
    56521 ataccgatcg agaaagtgcg ggttttaaat aaatctaatg aaattaatgg gtttggcgtg
    56581 tgtcgcgcat ggagaatata gagaagcagt aacccgatgc tggtgacaat taccgtcaag
    56641 gcaatccagc tggcgacaat cttttccccg aatagctcta ttccgcttga gaagagaaca
    56701 aggctgaggc caaacagcaa aaagccagtg atatcgaatc tgcgtcgtgc ggtggtgaaa
    56761 ttgggcatat gtttgcgcgc gtaaagaagg cccgcaatac ctatggggat attgattaaa
    56821 aatatccagt gccaggttgc ccaggtgacc agcacgccgc caagaacggg gcctaaaatt
    56881 ggccccacca gacccggcat ggcgacaaaa ttcaatactg gaagaagttc attacgagga
    56941 taagcgcgca gtaaggccag ccgagcaaca ggcatcatca ttgcgccgcc tatcccctga
    57001 ataacccgga agacaaccag ctgtggtagc gaattagaaa gtgcgcaggc cagagaaccc
    57061 aatgtgaaca gactcacggc aagggtaaaa atgcgacgcg taccgaagcg atcggctagc
    57121 catccgctta ccggaataag catcgccacc gtcagcgtat aactgatgat ggctgattgc
    57181 atcgcgagag gagaacgatt aaggctatga gcgattgcgg gtaaggcggt attcagaata
    57241 gtggcatcaa gtgcctgcat gaagaaggcc atcgccgcga tccacggcaa acccgccata
    57301 ctgcgattct ttttatcgct cattcaatgt cctgttatcg ggttgtcact tatcaggtga
    57361 gcgtagcagc gcctgacaag ctttaaatgc cgcgtcgcca tcgctttgga caatcgcatc
    57421 gacaatcgcc tggtgcagat ccagctttat cactgtgtcg ctggtaattg acgtgaagta
    57481 agtgtgataa accgaatgga atagcgaggc gaatgaggtc aaaaacggat tggcgctcat
    57541 ttcatagata tgctcatgcc aggccatatc gacttcgatc cagcgttcac ggcgaaagtt
    57601 ctcttttaat gccgccattt cggccattaa cgtattgaga tgcgccttct gttccgcggt
    57661 gccaaccgtt gcagccagta ggcaagcttg cggctccaga cagatacgca taaccagaaa
    57721 gtgatcgatg acctgatgaa agttctcttc tgtcatccac caggtaagca attcctgatc
    57781 aagaaaattc cagtttgatt gtggcatgac ccgggtacca attcgcggtc gcggtaaaac
    57841 catccctttt gccgttaacg ttttgaccgc ttcgcgtacc gctgtacgac tcactccaaa
    57901 ttgctcgccc agctcaatct caccaggcaa aatggtgccg ggttcatatt caccttttaa
    57961 gatgcgttgc gccagcttct cagccagaac atacgaaagg tttttctgtg cagctaactg
    58021 ttgtgcgctt aaaggcatta ctaatcttcc tttttctttt tattcctcct tagtatgcca
    58081 ccaggaagtg tgattacggt tgcaaaaacg gcaaattgct tgttttatgg cacattaacg
    58141 ggggttttgc t
//
LOCUS       DAZGSP010000018        57855 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZGSP010000018 DAZGSP010000000
VERSION     DAZGSP010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.018"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..455)
                     /gene="folK"
                     /locus_tag="ACHK74_001244"
     CDS             complement(<1..455)
                     /gene="folK"
                     /locus_tag="ACHK74_001244"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFP0220102.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRA"
     gene            complement(452..1849)
                     /gene="pcnB"
                     /locus_tag="ACHK74_001245"
     CDS             complement(452..1849)
                     /gene="pcnB"
                     /locus_tag="ACHK74_001245"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFP0220103.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1909..2805)
                     /gene="gluQRS"
                     /locus_tag="ACHK74_001246"
     CDS             complement(1909..2805)
                     /gene="gluQRS"
                     /locus_tag="ACHK74_001246"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFP0220104.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2872..3327)
                     /gene="dksA"
                     /locus_tag="ACHK74_001247"
     CDS             complement(2872..3327)
                     /gene="dksA"
                     /locus_tag="ACHK74_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFP0220105.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3505..4209)
                     /gene="sfsA"
                     /locus_tag="ACHK74_001248"
     CDS             complement(3505..4209)
                     /gene="sfsA"
                     /locus_tag="ACHK74_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFP0220106.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4224..4754)
                     /gene="thpR"
                     /locus_tag="ACHK74_001249"
     CDS             complement(4224..4754)
                     /gene="thpR"
                     /locus_tag="ACHK74_001249"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFP0220107.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4828..7257
                     /gene="hrpB"
                     /locus_tag="ACHK74_001250"
     CDS             4828..7257
                     /gene="hrpB"
                     /locus_tag="ACHK74_001250"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFP0220108.1"
                     /translation="MSSLPVAAVLSELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKDLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPRHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7453..9987
                     /gene="mrcB"
                     /locus_tag="ACHK74_001251"
     CDS             7453..9987
                     /gene="mrcB"
                     /locus_tag="ACHK74_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFP0220109.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10207..12396
                     /gene="fhuA"
                     /locus_tag="ACHK74_001252"
     CDS             10207..12396
                     /gene="fhuA"
                     /locus_tag="ACHK74_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFP0220110.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12447..13244
                     /gene="fhuC"
                     /locus_tag="ACHK74_001253"
     CDS             12447..13244
                     /gene="fhuC"
                     /locus_tag="ACHK74_001253"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFP0220111.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSCCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13244..14134
                     /gene="fhuD"
                     /locus_tag="ACHK74_001254"
     CDS             13244..14134
                     /gene="fhuD"
                     /locus_tag="ACHK74_001254"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFP0220112.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSESPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14131..16113
                     /gene="fhuB"
                     /locus_tag="ACHK74_001255"
     CDS             14131..16113
                     /gene="fhuB"
                     /locus_tag="ACHK74_001255"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFP0220113.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DINVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFVLAGGVLLLMAVVVALSFGRDAHGWTWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16148..17428)
                     /gene="hemL"
                     /locus_tag="ACHK74_001256"
     CDS             complement(16148..17428)
                     /gene="hemL"
                     /locus_tag="ACHK74_001256"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFP0220114.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17594..17641
                     /gene="yadX"
                     /locus_tag="ACHK74_001257"
     CDS             17594..17641
                     /gene="yadX"
                     /locus_tag="ACHK74_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFP0220115.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17653..19074
                     /gene="clcA"
                     /locus_tag="ACHK74_001258"
     CDS             17653..19074
                     /gene="clcA"
                     /locus_tag="ACHK74_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFP0220116.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19098..19163
                     /gene="yadW"
                     /locus_tag="ACHK74_001259"
     CDS             19098..19163
                     /gene="yadW"
                     /locus_tag="ACHK74_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFP0220117.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19156..19500
                     /gene="erpA"
                     /locus_tag="ACHK74_001260"
     CDS             19156..19500
                     /gene="erpA"
                     /locus_tag="ACHK74_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFP0220118.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19547..20170)
                     /gene="yadS"
                     /locus_tag="ACHK74_001261"
     CDS             complement(19547..20170)
                     /gene="yadS"
                     /locus_tag="ACHK74_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFP0220119.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20208..21008)
                     /gene="btuF"
                     /locus_tag="ACHK74_001262"
     CDS             complement(20208..21008)
                     /gene="btuF"
                     /locus_tag="ACHK74_001262"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFP0220120.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21001..21699)
                     /gene="mtnN"
                     /locus_tag="ACHK74_001263"
     CDS             complement(21001..21699)
                     /gene="mtnN"
                     /locus_tag="ACHK74_001263"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFP0220121.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21783..23300
                     /gene="dgt"
                     /locus_tag="ACHK74_001264"
     CDS             21783..23300
                     /gene="dgt"
                     /locus_tag="ACHK74_001264"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFP0220122.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23430..24854
                     /gene="degP"
                     /locus_tag="ACHK74_001265"
     CDS             23430..24854
                     /gene="degP"
                     /locus_tag="ACHK74_001265"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFP0220123.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            25009..26166
                     /gene="cdaR"
                     /locus_tag="ACHK74_001266"
     CDS             25009..26166
                     /gene="cdaR"
                     /locus_tag="ACHK74_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFP0220124.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26255..26641)
                     /gene="yaeH"
                     /locus_tag="ACHK74_001267"
     CDS             complement(26255..26641)
                     /gene="yaeH"
                     /locus_tag="ACHK74_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFP0220125.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26956..27780)
                     /gene="dapD"
                     /locus_tag="ACHK74_001268"
     CDS             complement(26956..27780)
                     /gene="dapD"
                     /locus_tag="ACHK74_001268"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFP0220126.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27811..30483)
                     /gene="glnD"
                     /locus_tag="ACHK74_001269"
     CDS             complement(27811..30483)
                     /gene="glnD"
                     /locus_tag="ACHK74_001269"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFP0220127.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30545..31339)
                     /gene="map"
                     /locus_tag="ACHK74_001270"
     CDS             complement(30545..31339)
                     /gene="map"
                     /locus_tag="ACHK74_001270"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFP0220128.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31707..32432
                     /gene="rpsB"
                     /locus_tag="ACHK74_001271"
     CDS             31707..32432
                     /gene="rpsB"
                     /locus_tag="ACHK74_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFP0220129.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32690..33541
                     /gene="tsf"
                     /locus_tag="ACHK74_001272"
     CDS             32690..33541
                     /gene="tsf"
                     /locus_tag="ACHK74_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFP0220130.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33688..34413
                     /gene="pyrH"
                     /locus_tag="ACHK74_001273"
     CDS             33688..34413
                     /gene="pyrH"
                     /locus_tag="ACHK74_001273"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFP0220131.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34705..35262
                     /gene="frr"
                     /locus_tag="ACHK74_001274"
     CDS             34705..35262
                     /gene="frr"
                     /locus_tag="ACHK74_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFP0220132.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35354..36550
                     /gene="ispC"
                     /locus_tag="ACHK74_001275"
     CDS             35354..36550
                     /gene="ispC"
                     /locus_tag="ACHK74_001275"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFP0220133.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36739..37497
                     /gene="ispU"
                     /locus_tag="ACHK74_001276"
                     /gene_synonym="uppS"
     CDS             36739..37497
                     /gene="ispU"
                     /locus_tag="ACHK74_001276"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFP0220134.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37510..38367
                     /gene="cdsA"
                     /locus_tag="ACHK74_001277"
     CDS             37510..38367
                     /gene="cdsA"
                     /locus_tag="ACHK74_001277"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP0220135.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38379..39731
                     /gene="rseP"
                     /locus_tag="ACHK74_001278"
     CDS             38379..39731
                     /gene="rseP"
                     /locus_tag="ACHK74_001278"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFP0220136.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39761..42193
                     /gene="bamA"
                     /locus_tag="ACHK74_001279"
     CDS             39761..42193
                     /gene="bamA"
                     /locus_tag="ACHK74_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFP0220137.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42315..42800
                     /gene="skp"
                     /locus_tag="ACHK74_001280"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42315..42800
                     /gene="skp"
                     /locus_tag="ACHK74_001280"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFP0220138.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42804..43829
                     /gene="lpxD"
                     /locus_tag="ACHK74_001281"
     CDS             42804..43829
                     /gene="lpxD"
                     /locus_tag="ACHK74_001281"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFP0220139.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43934..44389
                     /gene="fabZ"
                     /locus_tag="ACHK74_001282"
     CDS             43934..44389
                     /gene="fabZ"
                     /locus_tag="ACHK74_001282"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP0220140.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44393..45181
                     /gene="lpxA"
                     /locus_tag="ACHK74_001283"
     CDS             44393..45181
                     /gene="lpxA"
                     /locus_tag="ACHK74_001283"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFP0220141.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45181..46329
                     /gene="lpxB"
                     /locus_tag="ACHK74_001284"
     CDS             45181..46329
                     /gene="lpxB"
                     /locus_tag="ACHK74_001284"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFP0220142.1"
                     /translation="MTDQRSLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46326..46922
                     /gene="rnhB"
                     /locus_tag="ACHK74_001285"
     CDS             46326..46922
                     /gene="rnhB"
                     /locus_tag="ACHK74_001285"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFP0220143.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            46959..50441
                     /gene="dnaE"
                     /locus_tag="ACHK74_001286"
     CDS             46959..50441
                     /gene="dnaE"
                     /locus_tag="ACHK74_001286"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFP0220144.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50454..51413
                     /gene="accA"
                     /locus_tag="ACHK74_001287"
     CDS             50454..51413
                     /gene="accA"
                     /locus_tag="ACHK74_001287"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFP0220145.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51512..53653
                     /gene="ldcC"
                     /locus_tag="ACHK74_001288"
     CDS             51512..53653
                     /gene="ldcC"
                     /locus_tag="ACHK74_001288"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFP0220146.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53710..54099
                     /gene="yaeR"
                     /locus_tag="ACHK74_001289"
     CDS             53710..54099
                     /gene="yaeR"
                     /locus_tag="ACHK74_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP0220147.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54164..55462
                     /gene="tilS"
                     /locus_tag="ACHK74_001290"
     CDS             54164..55462
                     /gene="tilS"
                     /locus_tag="ACHK74_001290"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFP0220148.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55511..55771)
                     /gene="rof"
                     /locus_tag="ACHK74_001291"
     CDS             complement(55511..55771)
                     /gene="rof"
                     /locus_tag="ACHK74_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFP0220149.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55758..55958)
                     /gene="yaeP"
                     /locus_tag="ACHK74_001292"
     CDS             complement(55758..55958)
                     /gene="yaeP"
                     /locus_tag="ACHK74_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFP0220150.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56124..56669
                     /gene="yaeQ"
                     /locus_tag="ACHK74_001293"
     CDS             56124..56669
                     /gene="yaeQ"
                     /locus_tag="ACHK74_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFP0220151.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHMGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56666..57088
                     /gene="arfB"
                     /locus_tag="ACHK74_001294"
     CDS             56666..57088
                     /gene="arfB"
                     /locus_tag="ACHK74_001294"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFP0220152.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLADSHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57102..57812
                     /gene="nlpE"
                     /locus_tag="ACHK74_001295"
                     /gene_synonym="cutF"
     CDS             57102..57812
                     /gene="nlpE"
                     /locus_tag="ACHK74_001295"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFP0220153.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPLTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
BASE COUNT        13645 a        14287 c        16066 g        13857 t
ORIGIN      
        1 gctcttgtat gtaagacttc acgcaacgtc tccccatcag gaaacgccag ctccggtgca
       61 atctcaaaca gcggccacag cataaatcca cgattcttca tatcgtagtg cggaacggtc
      121 aggcgttcgg tatttatcac ttcattacca aacagcatga tatcgagatc cagcgtgcgt
      181 ggtccccagc gttcagcttt acgaacacga ccctgttgca gttctatgcg ctgtgtatgg
      241 ttgagtaact cttccggggc gagcgaagtt tcaagcgcca cggcggcgtt gaggtaatcg
      301 ggttgatctt gcggccccag cggtggggtg cggtaaaacg aagaaacggc aagaatgtgg
      361 ctttcaggga tatcgcctaa tgctttcagg gcagcattga cctgctccag cggagaggcc
      421 agattgctgc ctatggcaat atacgccact gtcatgcggt accctcacga cgcggtgcgc
      481 gtttgcgtgg acgacgagta cggcgacgcg gtgacggctc ttcatccaac tcgttaagca
      541 tccctttttg atctggtggc gcggaaacct ggaactcacc ccaccatttc accagacgct
      601 gcagttcagc gttacgttca acttcagctc gcaaggccaa caggtcataa gccgcacgga
      661 acttaggatg ctccagcagt ttccatgcgc gtttaccctg acgacgggac atacgcaact
      721 gcaactgcca gatatcgcgg gttaacgtcg tcagacgttt cgggattgcc agtgaacggc
      781 aggcttcgtc cagcacgtcg ttcatcgcca gcgcgaaagc gtcgtgatag gtcaggccgc
      841 tttcctgggc aatcttctgt gccgtctcca gcagtgggta ccagaacatg gcggcaaaca
      901 ggaacgccgg gttcacgcgc atatcgttat ggatacgcgt atcggtattc ttcagcacct
      961 gttcaatgat ccgctccatc gggctgtcgc cattttccgt gaagtagcgg gtaatggtcg
     1021 ggaacagcgg ctggaacaga tgatattcac acaacagctt ataggtatcg taaccgtagc
     1081 ccgcttgtag cagtttaagc gattcttcaa acaggcgtgc cggtgggata tcgttcaaca
     1141 gggtagcgag gcgagggatc ggttctgcgg tttccgggct gatgcgcata cccaatttgg
     1201 cggcaaaacg taccgcgcgc agcatacgta ccggatcttc acggtagcgc gtttccgggt
     1261 taccaatcag acggataacg ccgtccttca gatccttcat accgccaacg taatcacgga
     1321 cggtaaaatc cgctacgctg taatagaggc tgttgatagt gaaatcgcgg cgctgggcgt
     1381 cttcttcgat ggagccgaaa atgttgtcgc gcagcaacat gccgttttgc ccgcgttggg
     1441 aggtcgtgcg gtcgctgacg ttaccttcgt ggtgtccacg gaaggtcgca acttcgataa
     1501 tctccgggcc aaacattaca tgagccagac ggaaacggcg acccaccagg cggcagttac
     1561 ggaacagttt gcgcacctgc tcaggcgtgg cgttagtggt tacgtcaaaa tctttcggct
     1621 ttttgccaag taacaggtcg cgcacgccgc cgccaaccag ccaggcttcg tatcccgctt
     1681 tgttgagcct gtacattacc ttcagggcat tttcactgat atctttgcgg gaaatagcat
     1741 gctgctcacg cgggatcacc gtcacctgtg gacgggcgac tgcctgttca gcctcgcttt
     1801 cctcgcggct tagcaccttg cggcaaaaat tagcgactcg ggtaaaaata gtacacctcg
     1861 gtagtgtcaa acatcattca ggacaaaaaa atagcggcta atcatagctc agcatgacgc
     1921 atttgagaat gttgaattta caattgccga ctcgggcacg gcggtaagcc gccagttttt
     1981 gacggctgac tgaaggattt gctcgacgct gaaatcctgc cagtgtgctt ctgcctgctg
     2041 ccccagaaat tgaagtgccg cgattagtac cgggcgtgga tcgcctttcg gcaacgcagg
     2101 cgcatgattc tgcttggaaa gtttagcgcc ttgtggatta agcgccagcg gcagatgaat
     2161 gtaatctggc actttccagc caaaaagctg gtacagcgag atttgcctta ctgttggttc
     2221 aatcagatca gccccacgca ctatttctgt aacgccctgg aaatgatcat caaccacaac
     2281 agccaggttg taggcgaaca acccatcacg gcgatgaatg ataaaatctt cccgtgccag
     2341 tttttcgtcg gcgtgaataa tgccgcgcag ctggtcagta aattgcgtga ccggatgctg
     2401 ctggcggata cgcactgcgg cattgtctgg tccatgatgc aacacccggc aatgaccgtc
     2461 gtaaataccg ccaatgcttt gaatacgcgc acgcgtacag gtgcagtaat aacttagtcc
     2521 ttgttcatgt aaccaggcga gtgcttcacg ataggcgtgg tgacgttgcg attgccagag
     2581 aacatcgccg tcccagtgca gaccgtaatg ttccagctgg cgcaggatag tttctgcggc
     2641 accgggaact tcacgaggcg ggtcgatatc ttctatgcgt accagccagc gaccttgccg
     2701 ggcgcgagcc tgcaaatagc tgccgagcgc ggcgatcaga gagccaaaat gaagctcgcc
     2761 ggaaggagag ggggcgaagc ggccaatata ctgtgtgtct gtcatctctt tgaacaaaaa
     2821 ataaggcggg agcatttccc gcctgtggta aacgtgatgg aacggctgta attagccagc
     2881 catctgtttt tcgcgaattt cagccagcgt tttgcagtcg atgcacagat cggctgtcgg
     2941 gcgcgcttcc agacggcgaa taccaatttc aacaccgcag gattcgcagt agccgaaatc
     3001 ttcgtcttcc acttttttca gcgtcttctc gatctttttg atcagcttac gctcgcgatc
     3061 gcggttacgc agttcgaggc tgaactcttc ttcctgggct gcacggtcta ccgggtccgg
     3121 gaagttggct gcttcatcct gcatatgtgt aacggtgcga tcgacttcat ccctgagttg
     3181 attacgccat gcttccagaa tacgacggaa gtgcgccagc tgggcttcat tcatatactc
     3241 ttcgcccggc ttctcctgat atggttccac cccagcgatg gcgagaatac tcagggacga
     3301 tgttttacgg ttttgccctt cttgcatgtt gcttctcctt aacacgcact atcgatcccc
     3361 atgttcgggg gaaaaatgag gccgctataa atagcagatg cttttccgga tagcaattat
     3421 ctaaacgtaa cacttgacaa ctgtgtgagg aaaagcgtat ttgcgcacgc gaccagaatg
     3481 taaattaacc agttacttac tttactacaa tgtaaccggc agtgattttt taagagccat
     3541 gccttcagca gaaatttccg ctttgtaagc cagaatttct acccccctct gttgagcttc
     3601 tgacaatagt tgcgcgtatt tctcatcgat gtggcgcgcg ggtgaaaacc gtgtaatggc
     3661 tgaatgcagc acggcgaaaa agataaccgc acgctggcct tcagccgcta cgctcatcaa
     3721 ctcccgaagg tgtttctgac ctcgttcagt gaccgcatcg ggaaaatatc cctgttcgtt
     3781 ctccgctaac gtaaccgatt tcacttcaat atagcagtct ggacgcgaat ccgcctgcaa
     3841 cataaagtca atacggctgc gttctgcgcc gtattttact tcgcttttca gcgagctata
     3901 gcctgacagt tctgaaattg attcattaag gatagcctct ttcgtcaacc tgttagccca
     3961 aagcgtgttg acgcaaataa atgcgccgct ctggctttga gttaattccc aggtgtgtgg
     4021 gtatttccgt ttggtgttgt ctgaagtcga ataccagacg gtatcgccag gcgttgcaca
     4081 accggtcatt gcacccgtat tcgggcagtg tagcgttaat tcgcgaccat cgggtgtgat
     4141 cacatcggct aaaaaacgtt tgtaacgctg aattagcgtc gcgcgctgta gagggggaga
     4201 aaattccatt gcgacaatcc ttgttattgc gttagcgccc agcgttttag cggcgtgtag
     4261 cgtgtgcgtc cacgggcaaa cgaggaggcg taaagggtga actccgtcac cgcataagac
     4321 cagttaaaac ctggcggcgg gattgtcacc gcctcgctgg cgtcgcgcaa taaggtaata
     4381 tgtggatgaa acggacgatt gctttgaaaa caaccgctgc gggcagcctg tgaacggagc
     4441 atattcgcca gctggattaa gccacgtgga ggctgacgca tccctaacca caccacacgc
     4501 gaacgcagcc attgtccggc gtcatcaagc gtgagtgtga aaccaggttg acgaatccgt
     4561 ccggctaaaa gagaaagcgc cttctctttc tcggcgctca cttcgcctaa aaatgccaga
     4621 gtcagatgca aattatcggc ggcgaccgga cgtcccgcct caggtgggaa gtgtgtggcg
     4681 cgccaatgga taatctgttc gcggatttct gcaggtaagt cgatagcaaa gaacagacgt
     4741 tgcggttcag acatgtgagg cactcggtta tgaattaccg cgatgctaca atgtggcgcg
     4801 aagaatgtta accctctgga gcgttttgtg tcgtcgttgc ccgttgctgc cgtcttatct
     4861 gaattactta ccgctctcga ttgtgcgccg caggtattat taagtgcgcc gaccggggcc
     4921 gggaaatcaa cctggctgcc gctgcaactg ctggcgcatc ccggcattaa cgggaaaatt
     4981 atcctgctgg agccgcgtcg tctggcggcg cgtaacgtcg cgcaacggct ggcggagctg
     5041 cttaacgaaa agccaggcga taccgttggc taccggatgc gtgcgcaaaa ctgcgtcggg
     5101 ccgaataccc gcctggaagt ggttaccgaa ggcgtgctga cgcgcatgat ccagcgtgac
     5161 ccggaactga gcggtgttgg actggtgatc cttgatgaat ttcatgagcg cagcttgcag
     5221 gcggatttgg cgttggcgct gttactcgat gtgcaacaag gtctgcgtga tgaccttaaa
     5281 ctgctgatta tgtcggctac cctggacaac gaccgcttgc agcaaatgct gccagaagcg
     5341 cctgtcgtca tctcagaagg gcgctcgttt ccggttgaac gccgttattt accgctgccc
     5401 gcgcatcagc gttttgacga tgccgttgcg gtagccaccg ctgaaatgct gcgtcaggaa
     5461 agcggatcat tactgttatt tttacctggc gtcggagaaa ttcagcgtgt gcaggaacaa
     5521 ctggcttcgc gcatcggcag tgatgtattg ctctgcccgc tgtatggcgc gttgtcgctg
     5581 aacgatcagc gaaaagcgat cctcccggca ccgcaaggga tgcgcaaagt ggtgctggcg
     5641 accaatattg ctgaaaccag tttaaccatc gaaggtattc gtctggtggt ggattgtgcc
     5701 caggaaaggg tggcgcgttt tgatccacgc acggggctta cgcgactgat tactcaacgc
     5761 gttagccagg catccatgac gcagcgtgcc gggcgcgccg ggcgtctgga gccgggtatc
     5821 tgcctgcatt taatcgccaa agaacaagca gaacgcgctg ccgcgcaaag tgaaccggag
     5881 atcttacaaa gcgatctttc cggtttgctg atggaattac tgcaatgggg atgcagcgat
     5941 ccggcgcaga tgagctggct ggatcaaccg ccagtagtga atctactggc cgcgaaacgt
     6001 ctgttacaaa tgctgggggc gctggacggt gaacggctta gtgcgcaagg gcaaaaaatg
     6061 gcagcgctgg gtaacgatcc gcgtttagcg gcaatgctgg ttagcgcgaa gaacgacgac
     6121 gaagctgcta ccgcggcaaa aattgccgcc attctcgaag agccgccacg gatgggcaat
     6181 agtgacctgg gcgtggcgtt ttcgcgcaat caaccagcct ggcagcaacg tagtcagcaa
     6241 ctgttaaaac gcttaaacgt acgtggcggt gaggcagaca gttcgcttat cgcgccgcta
     6301 cttgccgggg cgtttgccga tcgcattgct cgtcgccgtg ggcaagatgg acgctatcaa
     6361 ctggcaaacg gcatgggagc gatgctcgat gccaacgacg cgctaagccg ccacgaatgg
     6421 ttgatcgcac cgttattatt gcagggcagc gcctcgccgg atgcgcggat tttactggcg
     6481 ctgccggtcg atattgatga gttagtacaa cgctgcccgc agctggtaca gcagtctgac
     6541 actgtggagt gggatgacgc gcaaggtacg ctgaaagcct ggcgtcggct gcaaatcggt
     6601 ctgctgacgg tgaaagtgca gccgctggcg aaaccctcgg aagacgagtt gcatcaggcg
     6661 atgcttaatg gcatccgcga taaagattta agcgtgctca actggacggc ggaagcggaa
     6721 cagctacgct tgcgtttgtt atgcgccgca aagtggttgc cggaatatga ctggccagcg
     6781 gttgatgatg aaagtttatt ggcgacgctg gaaacgtggc tgctgccaca tatgactggc
     6841 gtacattcac tacgcggcct gaaatcactc gacatttatc aggcactacg cggattactt
     6901 gattggggaa tgcagcaacg tctggatagt gaattgcctg cgcattacac tgtgccgacg
     6961 ggaagccgga tcgccattcg ttatcatgaa gataacccgc ccgcgctggc ggtgagaatg
     7021 caagagatgt ttggcgaggc caccaatccg acgatcgccc aggggcgcgt gccgctggtg
     7081 ctggagttgc tttcacctgc ccaaaggcca ttacaaatca cacgagattt gagcgccttc
     7141 tggaaaggag cgtaccgtga ggtgcaaaaa gagatgaaag ggcgttatcc cagacatgtc
     7201 tggccggacg acccggcaaa tactgcaccg acgcgacgga cgaaaaagta ttcgtaaggt
     7261 tggttttctc cctctccctg tgggagaggg ccggggtgag ggcatcagcg cgcacgttca
     7321 ccctcaccct aaccctctcc ctcaagggag aggggaccga tcgagcacaa attttgagag
     7381 atatcttctt ctgtcttgta acagaagaac agaaaatcgg gcttttgcgc ctgaatattg
     7441 cggagaaaaa gcatggccgg gaatgaccgc gagccaattg gacgcaaagg gaaaccgacg
     7501 cgtccggtca aacaaaaggt aagccgtcgt cgttacgaag atgacgatga ttacgacgat
     7561 tatgatgact atgaggatga agaaccgatg ccgcgcaaag gtaagggcaa aggcaaaggg
     7621 cgtaagcctc gtggcaaacg cggctggcta tggctgctgt taaaactggc tatcgttttt
     7681 gccgtgctga tcgccattta cggcgtttat ctcgatcaaa aaattcgtag ccgtattgat
     7741 ggcaaggtct ggcaactgcc tgcggcagtt tatggccgaa tggtcaacct tgagccagat
     7801 atgaccatca gcaagaacga gatggtgaag ctgctggagg cgactcagta tcgtcaggtg
     7861 tcgaaaatga cccgtcctgg cgaatttacc gtgcaggcca acagcattga gatgatccgc
     7921 cgtccgtttg atttcccgga cagtaaagaa gggcaggtgc gtgcgcgtct gacctttgat
     7981 ggcgatcatc tggcgacgat cgtcaatatg gagaacaacc gtcagttcgg tttcttccgt
     8041 cttgatccgc gtctgatcac catgatctcc tcgccaaacg gtgagcagcg tttgtttgtg
     8101 ccgcgcagtg gtttccctga tttgctggtg gatactttgc tggcgacaga agaccgtcat
     8161 ttttacgagc atgacggcat cagcctctac tccatcggac gtgcggtgct ggcaaacctg
     8221 accgccggac gcacggtgca gggagcgagt acgctgacgc aacagctggt gaaaaacctg
     8281 ttcctctcca gtgagcgttc ttactggcgt aaagcgaacg aagcttacat ggcgctgatc
     8341 atggacgcgc gttacagcaa agaccgtatt cttgagctgt atatgaacga ggtgtatctc
     8401 ggtcagagcg gcgacaacga aatccgcggc ttcccgctgg cgagcttgta ttactttggt
     8461 cgcccggtag aagagctaag cctcgaccag caggcgctgt tagtcggcat ggtgaaagga
     8521 gcgtccatct acaacccgtg gcgtaaccca aaactggcgc tggagcgacg taatctggtg
     8581 ctgcgtctgc tgcaacagca acagattatt gatcaagaac tctatgacat gttgagtgca
     8641 cgtccgctgg gggttcagcc gcgcggtggg gtaatctctc cacagccagc ctttatgcaa
     8701 ctggtgcgtc aggagctgca ggcaaaactg ggcgataagg taaaagatct ctccggcgtg
     8761 aagatcttca ctacctttga ctcggtggcc caggacgcgg cagaaaaagc cgccgtggaa
     8821 ggcattccgg cactgaagaa acagcgtaag ttgagcgatc ttgaaactgc gattgtggtc
     8881 gtcgaccgct ttagtggtga agttcgtgcg atggtcggtg gttctgagcc gcagtttgcg
     8941 ggctacaacc gtgcgatgca ggcgcgtcgt tcgattggtt cccttgcaaa accagcgact
     9001 tatctgacgg ctttaagcca gccgaaaatc tatcgtctga atacgtggat tgcggatgcg
     9061 ccaatcgcgc tgcgtcagcc gaatggccag gtctggtcac cgcagaacga tgatcgtcgt
     9121 tatagcgaaa gtggcagagt gatgctggtg gatgcgttga cccgttcgat gaacgtgccg
     9181 acggtaaatc tggggatggc gctggggctg cctgcggtta cggagacctg gatcaaactg
     9241 ggcgtgccga aagatcagtt gcatccggtt ccggcaatgc tgctgggggc gttgaactta
     9301 acgccaatcg aagtggcgca ggcattccag accatcgcca gcggtggtaa ccgtgcaccg
     9361 ctttctgcac tgcgttcggt aatcgcggaa gatggcaagg tgctgtatca gagcttcccg
     9421 caggcagaac gcgctgttcc ggcgcaggcg gcgtatctga cactatggac catgcagcag
     9481 gtggtacaac gtggtacggg tcgtcaactt ggggcgaaat acccgaacct gcatctggca
     9541 gggaaaacag ggactaccaa caataacgta gatacctggt ttgcgggcat tgacggcagc
     9601 acggtgacca tcacctgggt aggccgtgat aacaaccagc cgaccaaact gtatggtgcc
     9661 agcggggcaa tgtcgattta tcagcgttat ctggctaacc agacgccaac gccgctgaat
     9721 cttgttccgc cagaagatat tgcagatatg ggcgtggact acgacggcaa ctttgtttgc
     9781 agcggtggca tgcgtgtctt gccggtctgg accagcgatc cgcaatcgct gtgccagcag
     9841 agcgagatgc agcagcagcc gtcaggcaat ccgtttgatc agtcttctca gccgcagcaa
     9901 cagccgcaac agcaacctgc tcagcaagag cagaaagaca gcgacggtgt agccggttgg
     9961 atcaaggata tgtttggtag taattaacat ctaagcgtga aataccggat ggcgaattgc
    10021 catccggtaa aataacatcc cacctaaagt attaaccctt tcttttcatc tggttgttta
    10081 ttaacccttc aggaacgctc agattgcgta ccgcttgcga acccgccagc gtttcgaata
    10141 ttatcttatc tttataataa tcattctcgt ttacgttatc attcacttta catcagagat
    10201 ataccaatgg cgcgttccaa aactgctcag ccaaaacact cactgcgtaa aatcgcagtt
    10261 gtagtagcca cagcggttag cggcatgtct gtttatgcac aggcagcggt tgaaccgaaa
    10321 gaagacacta tcaccgttac cgctgcacct gcgccgcaag aaagcgcatg ggggcctgct
    10381 gcaactattg cggcgaagca ctctgctact gcgactaaaa cggatacacc aattgaaaaa
    10441 acgccgcagt ctatttcggt tgtgacaaat gaagagatgc agatgcatca atttcagtct
    10501 gtaaaagaag cattaggtta tacacctggc gttacggtta gcagtcgtgg tgcttctaat
    10561 acatatgatt ttgttatcat tcgtggtttc tcatctgttg gtctgaatca aaataattac
    10621 cttgatggac taaaacttca gggcaacttt tataacgatg ctgtgattga tccttacatg
    10681 ctcgagaggg ttgaactgat gcgtgggccg acgtctgttc tctatggaaa aagtaatcct
    10741 ggtgggatta tttctatggt gagtaagcgt ccgaccactg aacccctgaa agaaattcag
    10801 tttaaaatgg ggacggataa tctgtttcag accggattcg atttcagtga tgcactggat
    10861 gataacggtg agttctctta ccgtttgacg ggccttgctc gctcaacaaa cgaacagcag
    10921 aaaaactctg aatctcagcg ttataccatt gctccatcat tctcatggcg tccagacgac
    10981 aaaaccaatt ttactttcct gtcctatttc cagaatgaac cagaaacggg ttattacggt
    11041 tggttgccga aagaggggac cgttgagcca ttgcctaatg gtaagcgtct accgactgac
    11101 ttcaatgaag gtgcatcgaa taatacatac tcccgtaacc agaaaatggt gggatatagt
    11161 tttgaacatg gttttaatga caccttcacc gtgcgtcaga atctgcgttt cagtgaaatg
    11221 aaaacctcac agaaaagtgt ttatggcaca gggattgctg gtgatggtca taccctaaac
    11281 cgcgggacag tggtggataa tgagcgtcta caaaacttta gcgttgatac ccaacttgaa
    11341 agtaagtttg ctacaggtga cgtcgagcat actttgctga caggggtaga cttcatgcgg
    11401 atgcgtaatg atatcaatgc cagctttgga tccgcaccat ccatcgatct ttataacaaa
    11461 tatcaccctg aatactttgc atttggtagc gcagagccat accagatgaa tgaaagcaaa
    11521 caaacaggta tttatgttca ggatcaggcg gaatggaata aatgggtatt cactctgggg
    11581 ggacgctacg attggtctaa gcaagcgacc actgttcgtg aaaactctta tacgccgact
    11641 gaaggttata ttgagcgcaa tgatcatcag ttcacctggc gcggtggtgt aaattactta
    11701 ttcgataatg gtatttcacc ttactttagc tatagccagt cctttgaacc gagtgctttc
    11761 gatctgtgga gcaacccgcg catttcctat aagccatcga aaggtgaaca gtatgaagct
    11821 ggcgtaaaat atgttccgaa tgatatgccg gtcgttgtta cgggcgcagt ctatcaattg
    11881 acgaaaacaa ataacctgac agcagaccca acaaacccat tagcgcaagt cccagcaggt
    11941 gagattcgcg ctcgtggtgt ggaacttgaa gcaaaagcgg cgttaaatgc caatattaac
    12001 ttgacggctt cttataccta caccgatgcg gaatacacca aagacaccaa tctcaaaggt
    12061 aaaactccag aacaagtacc ggagcatatg gcatctctct ggggggatta taccttcaat
    12121 gaagggccgc tttctggttt aacattggga acaggtggtc gttttattgg ttccagctat
    12181 ggtgatccgg caaacacttt taaagtgggt agcgcagctg taatggatgc tgttgtaaaa
    12241 tatgatctgg cacgctttgg tatggcggga tccagccttg ctgtgaacgt caacaatttg
    12301 ctcgatcgtg agtatgttgc cagttgcttc cagacttatg gctgcttctg gggcgcagaa
    12361 cgtcaggtcg ttgcaaccgc aaccttccgt ttctaatttc tcttttgggg cacggatttc
    12421 cgtgcccatt tcacaagttg gctgttatgc aggaatacac gaatcattcc gataccactt
    12481 ttgcactgcg taatatctcc tttcgtgtgc ccgggcgcac gcttttgcat ccgctgtcgt
    12541 taacctttcc tgccgggaaa gtgaccggtc tgattggtca caacggttct ggtaaatcca
    12601 ctctgctcaa aatgcttggc cgtcatcagc ctccgtcaga aggggagatt cttcttgatg
    12661 cccagccgct ggaaagctgg agcagcaaag cgtttgcccg caaagtggct tatttgccgc
    12721 agcagcttcc tccggcagaa gggatgaccg tgcgtgaact ggtggcgatt ggtcgttacc
    12781 cgtggcatgg cgcgttgggg cgctttgggg cggcagatcg cgaaaaagtc gaggaagcta
    12841 tctcgctggt tggcttaaaa ccgctggcgc atcggctggt cgatagtctc tctggcggcg
    12901 aacgtcaacg ggcgtggatc gccatgctgg tggcgcagga tagctgttgt ctgttgctcg
    12961 acgaaccgac ttcggcgctg gatatcgccc accaggttga tgtgctggcg ctggtgcacc
    13021 gtttaagtca ggagcgtggc ctgacggtca ttgccgtgtt gcacgatatc aatatggcgg
    13081 cacgctactg tgattatctg gtcgccctgc gcggtggtga aatgattgct cagggaacgc
    13141 ctgcggaaat tatgcgcggc gaaactctcg aaatgattta tggcatcccg atgggtattt
    13201 tgccgcatcc ggcgggtgct gcacctgtga gttttgttta ttgatgagcg gcttacctct
    13261 tatttcgcgc cgtcgactgt taacggcgat ggcgctttct ccgttgttat ggcagatgaa
    13321 taccgcccac gcggcggcta ttgaccccaa tcgtattgtg gcgctggagt ggttgccggt
    13381 ggaattactg ctggcgctcg gcatcgtgcc ttacggcgtg gcggatacca tcaactatcg
    13441 cctgtgggtc agcgaatcac cattgccgga ctcagtgatt gacgtcggtt tgcgtacaga
    13501 acctaacctt gaactgctga ccgaaatgaa accgtcgttt atggtctggt cggcaggata
    13561 tggcccttca tcagaaatgc tggcacgtat tgcgccgggt cgcggattta acttcagcga
    13621 tggcaaacag ccgttggcga tggcgcgtaa atcgctgacg gaaatggcag atttacttaa
    13681 cctgcaaagc gcagcggaaa cgcatttagc gcaatacgaa gactttatcc gcagcatgaa
    13741 accccgcttt gtgaagcgtg gtgcacgccc gttactgctg acgacgctta tcgatccgcg
    13801 ccatatgctg gtcttcggtc caaacagctt gttccaggaa attctcgatg agtacggcat
    13861 cccaaatgcc tggcaagggg aaaccaactt ctggggcagt accgccgtca gtatcgatcg
    13921 tctggcggcg tataaagacg ttgatgtgct ctgttttgat cacgacaaca gcaaagacat
    13981 ggatgcgcta atggcaaccc cgctgtggca ggccatgccg tttgtccgcg ccggacgctt
    14041 tcagcgcgta cccgcagtct ggttttatgg tgcgacgctc tcggcaatgc actttgtgcg
    14101 cgttctggat aacgccatcg gaggtaaagc gtgagtaaac gaattgcgct ttttccggcg
    14161 ttattgctgg cgctgctggt gattgtcgct acggcgctga cctggatgaa cttttcgcag
    14221 gcgctgccgc gtagccagtg ggcgcaggcc gcctggtcgc cggatattaa cgtcatcgag
    14281 cagatgattt ttcactacag cttgttacca cgtctggcga tttcgctgct ggtgggcgca
    14341 ggcctggggc tggtgggcgt gctgtttcag caagtgctgc gtaatccgct ggcggaaccg
    14401 acgacacttg gcgttgctac aggcgcgcaa ctgggaatta ccgtcaccac actttgggcg
    14461 atcccaggtg ctatggcgag ccagtttgcc gcactggtgg gcgcttgtgt cgttggctta
    14521 atcgtctttg gcgtcgcgtg ggggaaacgg ctttcgccgg ttacgcttat tctcgcgggg
    14581 ctggtggtga gcctttattg cggggcaatt aatcagctac tggttatctt ccatcacgac
    14641 cagctgcaaa gcatgttcct gtggagcacc ggaacgctga cgcaaaccga ctggggcggc
    14701 gttgagcgtt tatggccgca gttgctgggc ggtgtgatgc tgacgttgct gctacttcgt
    14761 ccgttaaccc tgatggggct tgatgatggc gtggcgcgca atctcgggct ggccttgtcg
    14821 cttgcgcgtc tggcggcgct gtcgctggcg attgtcatca gtgcgctgct ggtgaacgct
    14881 gtggggatta tcggctttat cgggttgttc gcgccgctgc tggcaaaaat gctgggggcg
    14941 cggcgtctgc tgccacgact gatgctggcg tcgttgattg gtgcgctgat cctctggctt
    15001 tccgatcaaa tcatcctctg gctgactcgc gtttggatgg aagtgtccac cggttcggtc
    15061 actgcgttga tcggtgcgcc gctgctactg tggctgttgc cgcgtttacg cagcattagc
    15121 gcgccggata tgaaggtcaa cgatcgtgtc gcggctgaac gccaacatgt gctggcgttt
    15181 gtcctcgcgg gcggcgtgct gctgttgatg gctgtggtag tggcgctgtc gtttggtcgt
    15241 gatgcgcacg gctggacgtg ggcgagcggg gcgttgctcg atgatttaat gccctggcgc
    15301 tggccgcgaa ttatggcggc gctgtttgcg ggcgtcatgc tggcggtggc gggctgtatt
    15361 attcagcggc tgaccggcaa cccgatggca agcccggaag tgctggggat tagctccggc
    15421 gcagcgtttg gcgtggtgtt gatgctgttt ctggtgccgg gtaatgcctt tggctggctg
    15481 ttacctgcag gcagtctcgg ggcggcggtg acgctgttga tcattatgat cgccgccgga
    15541 cgcggtggat tttccccaca ccgcatgtta ctggcgggga tggcgttaag taccgcgttc
    15601 accatgcttt tgatgatgtt gcaggcaagt ggtgacccgc gaatggcgca agtgctgacc
    15661 tggatttccg gttcgaccta caacgcgacc gatgcgcagg tctggcgcac gggaattgtg
    15721 atggtaattt tgctggcgat taccccgctg tgccgccgct ggctgaccat tttaccgctg
    15781 ggtggtgata ccgcccgagc cgtaggaatg gcgctgacgc cgacgcgaat tgcgctgctg
    15841 ctgttagcgg cttgcctgac ggcgaccgcg acgatgacta ttggaccgtt gagttttgtt
    15901 ggtttaatgg caccgcatat tgcgcggatg atgggctttc gacggacgat gccacacatc
    15961 gtaatttcgg cgctggtggg tggtttactg ctggtattcg ctgactggtg tgggcggatg
    16021 gtgctgtttc cattccagat cccggcaggg ctgctgtcga cctttatcgg cgcgccatat
    16081 tttatctatt tgttgagaaa gcagagccgt taatagaatg cctcgcccgg tgggcgaggc
    16141 aaaatcatca caacttcgca aacacccgtc gtgcagcatc gatggtgtta ttgatatctt
    16201 ccatgctgtg cgccacggac ataaagcccg cttcaaacgc tgacggtgcc aggtaaacac
    16261 cttcgtccag catcatatgg aagaaacgct taaagcgttc cacgtcacag gccatcacat
    16321 cctgatagca cgtcacggac tcggcgtcgg taaagaaaat accgaacatg ccgccaacgt
    16381 ggttaacgac cagcggaatt ccggcttctt ctgccgcttc cagcagacct tctgccagac
    16441 gtgttgtcag ctcatccagc gtttcgtgaa cgcccggctg cgcgacttca ttcagacagg
    16501 cgaaacccgc tgccatcgca attgggttac cggaaagcgt acccgcctga tagaccggac
    16561 ccgtcggggc cagcgcatcc attacatcac gacgaccacc gaatgcgcct accggcattc
    16621 caccgccgat gattttgccc aggcaggtga gatccggttc cacaccgtaa taatcctgtg
    16681 cgccagctag cgctacgcgg aagccagtca ttacttcatc gatgatcagc aatgcgccaa
    16741 attcgtcgca cagcgcgcgc agacctggca ggaactctgg cagcggtgga acgcagttca
    16801 tattgcctgc caccggctca acgataatac aggcaatctc ttgcgggtat tgctcaaacg
    16861 cggcgcgtac agaagccaga tcgttataag tacaggttaa ggtatgtttg gcgaaatctg
    16921 ccggaacacc cggcgagttt ggctggccta acgtgagtgc gccagaaccg gctttcacca
    16981 gcaggcagtc agcgtgaccg tggtaacaac cttcaaattt aatgattttg tcgcgaccgg
    17041 taaaaccacg ggccaggcgg atggcgctca tcgtcgcttc agtgccggag ttcaccatgc
    17101 gcaccatatc catggtcggg accagttcgg tcaccagttg cgccattttc acttccattt
    17161 cggtcggtgc accaaagctt aaaccacgct cggcggcttc aatcacggca ttgcggatag
    17221 ccggatggtt atggcccagc accatcggcc cccaggaacc gacataatcg atataggctt
    17281 tgccatcaac atcgtacaga taagcgccgt ccgctttttc gataaacagt ggagtgccgc
    17341 ccacgccagt aaaggcgcga acaggggagt tcacaccgcc agggatcagc tcgcgcgctg
    17401 cgctgtaaag attttcagac ttactcatgg agggttcctg attcgtagaa aaagtgaatg
    17461 gctgctattc tatgttattc ataacaagtt aaatactcgt caaacatcag gctgcttgta
    17521 ctggtgcaat cctgatttct tagagtataa aagttttgtg catttgaaac attacgcttt
    17581 gcaaaggatt ttcatggaac gtgcgagtaa aatgccgtca tcttatttgt atgaccaata
    17641 agtgatcatt ggatgaaaac tgatactccc tctttagaaa caccgcaggc cgcgcgcctg
    17701 cgacgcagac aactgattcg ccaacttctt gagcgcgata aaaccccgtt agccattttg
    17761 tttatggcgg cagtcgtcgg cacgcttgtc gggctggctg cggttgcttt tgacaaaggt
    17821 gtcgcctggt tgcagaacca acgtatgggg gcgctggtac atactgctga taattatccg
    17881 ctactgttaa ccgtcgcttt tctctgttcg gcggtgctgg cgatgttcgg ctactttttg
    17941 gtgcgcaaat acgcgcccga agcaggggga tcggggatcc cggaaattga aggggcgctg
    18001 gaagatcaac gtcctgttcg ctggtggcgt gtattgccgg tgaaattctt tggcgggctg
    18061 gggacactcg gcggaggcat ggtgctaggg cgcgaaggac caaccgtgca gatcggcggc
    18121 aatatcggtc gcatggtgct tgatattttc cgcctgaaag gtgatgaagc tcgccatacg
    18181 ctgctggcaa ccggtgctgc tgcggggctg gctgcggcct ttaacgcgcc gctggcgggt
    18241 attttgttta ttatcgaaga gatgcgtccg cagtttcgct atacgttaat ttcgattaaa
    18301 gcggtattta ttggcgtcat tatgtcgacc attatgtacc ggatttttaa tcatgaagtt
    18361 gcgttgattg acgtcggtaa actttctgat gcgccgctta atacgctgtg gctttatctg
    18421 atcctcggta ttatttttgg cattttcggc cctattttta ataaatgggt gctggggatg
    18481 caggatttgc tgcaccgtgt gcacggcggc aatattacca aatgggtgct aatgggcggt
    18541 gcgattggcg gtctgtgtgg attgctgggg tttgtggcac cagcaacgtc gggcggcggt
    18601 tttaacctga ttcctatcgc taccgcgggg aatttcagca tgggaatgct ggtgtttatc
    18661 ttcgtcgcgc gggtcattac caccttactc tgcttctctt ccggcgcgcc gggcggtatt
    18721 tttgccccga tgctggcgct gggtactgtg ctgggaaccg ctttcggaat ggttgccgtt
    18781 gagctgtttc cgcaatatca ccttgaggcg ggaacgtttg ctattgccgg aatgggggcg
    18841 ttactggcgg catctattcg cgcgccgtta acggggatca ttctggttct ggagatgacc
    18901 gataactacc agctcatttt gccaatgatt attaccggtc ttggcgcaac actattagcg
    18961 caatttaccg gcgggaaacc gctatactcg gcgattcttg cgcgcacgct ggcaaaacag
    19021 gaagctgagc aactggcgcg aagcaaggcc gcatcagcca gcgagaatac ttgaacgaaa
    19081 taccagggta ttagataatg gcgattatta ttgggttaga atttgcccaa ttgccgatgt
    19141 cgtttggagc aaaatatgag tgatgacgta gcactgccgc tggagtttac cgacgcagca
    19201 gccaacaaag ttaaaagcct gatcgctgac gaagataacc cgaatctgaa attacgcgtg
    19261 tatatcaccg gtggcggttg cagcggcttc cagtatggtt tcacctttga tgatcaggtg
    19321 aacgaaggcg atatgaccat cgaaaaacag ggcgttggcc tggtggttga tccgatgagc
    19381 ctgcaatatc tggtcggcgg ttccgttgac tataccgaag gactggaagg ttctcgtttc
    19441 atcgtgacca acccgaacgc gaaaagcacc tgcggttgcg gttcttcctt tagcatctaa
    19501 tctgttgttc atgtgccgga taaactatcc ggcacatctt ctctcttcaa cgcccattct
    19561 catccagcgc aaacgtcggt agcttaagat gccaacgaat agccgccagc cgaatcaata
    19621 gcgtcacgac catgcccatc atactggctg tttccagtgg tacggaaaat gtgtaataag
    19681 ccgtagcgtg gacaataccg ccgataatac aggcagttgc gtagatttct gtacgtaaaa
    19741 tcatggggat ctcgcgggcc agtacatcac gaataatccc gccgccaacg ccagtaatga
    19801 cgcccataca aaccgcgatt aacggaccgg cttccgcatt aaaggcttta ttcacgccaa
    19861 tgccgacaaa caccgccaga ccaacggcgt ccagcaccgg caacatccat tttggtaagc
    19921 gtcttggctg gcgcaccagc acgatggtca gcatgctggt gaccattgca acgaccagat
    19981 cggtgggatc tttcacccaa aataccgggc cgtgatccag cgccatgtcg cgaattgttc
    20041 cgccgcctac tgcggttacc acgcccagta ccagaacacc aaaagggtcc atacgcaatt
    20101 ttccggctaa caaaacgccg gagatggcaa ataccgctgt gccgactata tccagccaat
    20161 agacgagcat tgttcaatcc ccactgagca cctgaaaagg tcaggcacta atctacctgt
    20221 gaaagcgcat tacagagctg ttgtgctgcg aggataatac gtgggcttgc acgttcaaac
    20281 cagtcactcg tgagaggaat aacgggaatt ttgagctgtt caccccagta ttgtttgatt
    20341 ttaggaattt ggtccggtcc gcctgtaatg acaatcgcct gtggcgagcg tgctaacacc
    20401 tgttcgcggc taacttgcgg ccagggaacc cggctgtctt taaagatgtt ttctccgcca
    20461 caaacttcga gtacctggtt ctgaatcgac tcttttccac tggtaaatgg cggattaatg
    20521 ccgaattgca gaaaaacacg ttttttaggt ttatcagcat attgcgcttt caattgcgcg
    20581 tactgatcca gcagggattg cgcggcttgt tcggccttgt ctggttgcgg actccagggg
    20641 gccagttgac gtaacgcatt ggcaatttgt tcaatgcttg tcgcatcgac ccacatcact
    20701 tttattccca gcgaagccag ctggtcaacc tgccgctcgg cattacctcc acgccaggca
    20761 atcaccagat cgggcttcag cgcgacaatg cgttccagat tcatcccctg ccaggtggaa
    20821 acctgctcaa tcttttgcgc ttgtggagga tagtcggaat agctgctgac cccaaccggc
    20881 gtgatcccgg cagcaaaggc aagttcagtg ttggcgggag aaagcgtgat gacgcgcggc
    20941 gcggcgttga gccacagtgg cgcaagaaaa gacagggcga ccagcgccct gaacagtgac
    21001 ttagccatgt gcaagtttct gcaccagtga ctcaaccatc aggctggact gtttagcggc
    21061 aacagccagg aactcatcga agctaagatg agactgttga tcggccacgt cggagatggc
    21121 gcgtacgaca acaaacggga cgttgaaatt gtggcagaca tgggcgattg ccgtcgcttc
    21181 catctctaca gcaatggcct gtgggaagtt gtggcggatt ttcgccagac caacagaacc
    21241 gttgatgaaa gcgtcgccgc taacaatcag gccacgtaca gcgttaagat tcagttcggc
    21301 aatgcaggcc tcagcggcag cgatcagttt atcgtcagct ttaaagcctg ccggacagcc
    21361 tggtaactga ccgtattcat aaccaaatgc cgtgacatcc gcgtcgtgat aacgtgcttc
    21421 gtccgagaca acgatatcgc ccactttcaa cgttggtgcc aggccaccgg cagaaccggt
    21481 gttaataatc acatctggct tgcagtgttc caacagcaaa gtggcaccca gcgccgcagc
    21541 gactttaccg atgcccgatt tcagaagcgc aacctcggtt ccattcagtt ggccggtata
    21601 gatttcgcaa ccgccgagac tgatagtttg acggttttcg attttgtcac gcagcagcgt
    21661 aacttcttct tccattgcac caatgatgcc gattttcata gatttactcg cgataagccc
    21721 gatttgaagg catagtttac catgcgctta cggggaagcg tatttctcac gcgggagagg
    21781 acatggcaca gattgatttc cgaaaaaaaa taaactggca tcgtcgttac cgttcaccgc
    21841 agggcgttaa aaccgaacat gagatcctgc ggatcttcga gagcgatcgc gggcgtatca
    21901 tcaactctcc ggcaattcgt cgtctgcaac aaaagaccca ggtttttcca ctggagcgca
    21961 atgccgccgt gcgcacgcgt cttacccact cgatggaagt ccagcaggtg gggcgctaca
    22021 tcgccaaaga aattttaagc cgtctgaaag agcttaaatt gctggaagca tacggcctgg
    22081 atgaactgac cggacctttt gaaagcattg ttgagatgtc atgcctgatg cacgatatcg
    22141 gcaatccgcc gtttggtcat tttggcgaag cggcgataaa tgactggttt cgccagcgtt
    22201 tatacccgga agatgccgaa agccagcctc tgactgacga tcgctgcagc gtggcggcac
    22261 tacgtttacg ggacggggaa gaaccgctta acgagctgcg gcgcaagatt cgtcaggact
    22321 tatgtcattt tgaggggaat gcacaaggca ttcgtctggt gcatacattg atgaggatga
    22381 atctcacctg ggcacaggtt ggcggtattt taaaatatac ccgtccggcg tggtggcgtg
    22441 gcgaaacgcc tgagacacat cactatttaa tgaaaaagcc gggttattat ctttctgaag
    22501 aagcctatat tgcccggttg cgtaaagaac ttaatttggc gctttacagt cgttttccat
    22561 taacgtggat tatggaagct gccgacgaca tctcctattg tgtggcagac cttgaagatg
    22621 cggtagagaa aagaatattt accgttgagc agctttatca tcatttgcac gaggcgtggg
    22681 gtcagcatga gaaaggttcg ctcttttcgc tagtggttga aaatgcctgg gaaaaatcac
    22741 gctcaaatag tttaagccgc agtacggaag atcagttttt tatgtattta cgggtaaaca
    22801 ccctaaataa actggtaccc tacgcggcac aacgatttat tgataatctg cctgcgattt
    22861 tcgccggaac gtttaatcat gcattattgg aagatgccag cgaatgcagc gatcttctta
    22921 agctatataa aaatgtcgct gtaaaacatg tgtttagcca tccagatgtc gagcagcttg
    22981 aattgcaggg ctatcgggtc attagcggat tattagagat ttatcgtcct ttattaagcc
    23041 tgtcgttatc agactttact gaactggtag aaaaagaacg ggtgaaacgt ttccctattg
    23101 aatcgcgctt attccacaaa ctctcgacgc gccatcggct ggcctatgtc gaggctgtca
    23161 gtaaattacc gtcagattct cctgagtttc cgctatggga atattattac cgttgccgcc
    23221 tgctgcagga ttatatcagc ggtatgaccg acctctatgc gtgggatgaa taccgacgtc
    23281 tgatggccgt agaacaataa ccaggctttt gtaaagacga acaataaatt tttacctttt
    23341 gcagaaactt tagttcggaa cttcgcgcta taaaacgaat ctgaagaaca cagcaatttt
    23401 gcgttatctg ttaatcgaga ctgaaataca tgaaaaaaac cacattagca ctgagtgcac
    23461 tggctctgag tttaggtttg gcgttatctc cgctctctgc aacggcggct gagacttctt
    23521 cagcaacgac agcccagcag atgccaagcc ttgcaccgat gctcgaaaag gtgatgcctt
    23581 cagtggtcag cattaacgta gaaggtagca caaccgttaa tacgccgcgt atgccgcgta
    23641 atttccagca gttcttcggt gatgattctc cgttctgcca ggaaggttca ccgttccaga
    23701 gctctccgtt ctgccagggg ggccagggcg gtaatggcgg cggccagcaa cagaaattca
    23761 tggcgctggg ttccggcgtc atcattgatg ccgataaagg ctatgtcgtc accaacaacc
    23821 acgttgttga taacgcgacg gtgattaaag tccagctgag cgatggtcgt aagttcgacg
    23881 cgaagatggt tggcaaagat ccgcgctctg atatcgcgct gattcagatc cagaacccga
    23941 aaaacctgac cgcaattaag atggcggatt ctgatgcact gcgcgtgggt gattacaccg
    24001 tagcgattgg taacccgttt ggcctgggag agacggtaac ttccgggatt gtctctgcgc
    24061 tggggcgtag cggcctgaat gccgaaaact acgaaaactt catccagacc gatgcagcga
    24121 tcaaccgtgg taactccggt ggtgcgctgg ttaacctgaa cggcgaactg atcggtatca
    24181 acaccgcgat cctcgcaccg gacggcggca acatcggtat cggttttgct atcccgagca
    24241 acatggtgaa aaacctgacc tcgcagatgg tggaatacgg ccaggtgaaa cgcggtgagc
    24301 tgggtattat ggggactgag ctgaattccg aactggcgaa agcgatgaaa gttgacgccc
    24361 agcgcggtgc tttcgtaagc caggttctgc ctaattcctc cgctgcaaaa gcgggcatta
    24421 aagcgggtga tgtgatcacc tcactgaacg gtaagccaat cagcagcttt gccgcactgc
    24481 gtgctcaggt gggtactatg ccggtgggca gcaaactgac cctgggcttg ctgcgcgacg
    24541 gtaagcaggt caacgtgaat ctggaactgc agcagagcag ccagaatcag gttgattcca
    24601 gctccatctt caacggcatt gaaggtgctg agatgagcaa caaaggcaaa gatcagggcg
    24661 tggtagtgaa caacgtgaaa acgggcactc cggctgcgca gatcggcctg aagaaaggtg
    24721 atgtgattat tggcgcgaac cagcaggcag tgaaaaacat cgctgaactg cgtaaagttc
    24781 tcgacagcaa accgtctgtg ctggcactga acattcagcg cggcgacagc tccatctacc
    24841 tgttaatgca gtaatctccc tcaacccctt cctgaaaacg ggaaggggtt ctccttacaa
    24901 tctgtgaact tcaccacaac tccatacatc ttcatcatcc tttaggcatt tgcacaatgc
    24961 cgtacgttac gtacttcctt atgctaagcc gtgcataacg gaggacttat ggctggctgg
    25021 catcttgata ccaaaatggc gcaggatatc gtggcacgta ccatgcgcat catcgatacc
    25081 aatatcaacg taatggatgc ccgtgggcga attatcggca gcggcgatcg tgagcgtatt
    25141 ggtgaattgc acgaaggtgc attgctggta ctttcacagg gacgagtcgt cgatatcgat
    25201 gacgcggtgg cacgtcatct gcacggtgtg cggcaaggga ttaatctacc gttacggctg
    25261 gaaggtgaaa ttgtcggcgt aattggcctg acaggtgaac cagagaatct gcgtaaatat
    25321 ggcgaactgg tctgcatgac ggctgaaatg atgctggaac agtcgcggtt gatgcacttg
    25381 ttggctcagg atagccgttt gcgggaggaa ctggtgatga acctgattca ggcagaggag
    25441 aatactcccg cacttactga atgggcgcaa cggctgggga tcgatctcaa tcaaccgcga
    25501 gtggtggcta ttgttgaggt cgacagcggt cagcttggtg tggacagcgc aatggcggag
    25561 ttacaacaac tgcaaaacgc gctgactacg cccgagcgta ataatctggt ggcgattgtc
    25621 tcgctaaccg aaatggtggt gttgaaaccg gcgttgaact cttttgggcg ctgggatgca
    25681 gaagatcatc gtaagcgagt tgaacaactg attacccgca tgaaagagta cggccagctg
    25741 cgttttcgcg tttcactggg caactatttt accggtcctg gcagtattgc ccgatcctat
    25801 cgtacggcga aaacgacgat ggtggtgggt aaacagcgga tgccagaaag tcgctgctat
    25861 ttttatcagg atctgatgtt acctgtgtta ctcgacagtt tgcgtggcga ctggcaggcc
    25921 aacgaactgg cgcgaccgct ggcgcggctg aaagcgatgg acaataacgg cttgctgcga
    25981 cgaacgctgg cggcgtggtt tcgtcacaat gtgcaaccgc tggcaacgtc aaaggcgttg
    26041 tttattcatc gtaatactct ggagtatcgg cttaatcgta tatcggaact gaccgggctt
    26101 gatttgggta attttgatga caggttgctg ctgtatgtgg cgttgcagct ggatgaagag
    26161 cggtaggtta tgcgttaagg tcgaggcggc gctggctcat tcccctcagc ccgaagggta
    26221 gaggggaatg tccgagttaa ccgtcagttc ggcattattt acgcgttagt ttttccagat
    26281 cggcttcaat ctcgctgatc ttattggtta cgactgactc caggtgacgt aagtcgtcga
    26341 ggatcttacg cttaagatca acttcgctgc ggtcacgctg gcagatttga tcaagctcat
    26401 caatgatata ccgtagattc gggctgattt cctggacttc tttataaccc tgacccacac
    26461 catcagcgac gaccgtttta cgctgacgcg gatatttaaa cttaacgctc ttggcgaaaa
    26521 actcgccttt gtctttctgg aaatagattt tcagaatatc gttgttggct tcctgccgga
    26581 ggctgtaacg atcaatttct tcaggattgg taatacccag acttttcaga ttgtcgtaca
    26641 tagcgttacc tcaaaatgag tcagtaaatt gtgcttattt tagcatttgg cctggcccgc
    26701 ccggctcgac tgtgatcgca gaaagccttt ttcttccaga cttctatcat ggcgtaaaaa
    26761 tcaaaaaatt acctgcttta ttgtggcgat attccatggg atggattaac acatttatct
    26821 tcgttttgcg aattacatga cagtccgaaa caagatgaaa aagtatttaa aaaagggggg
    26881 aagaagaatg agtaggacga attattctga gccagaaagc atacgggcag catcatgctg
    26941 cccgtatatg tactcttaat cgatggtacg cagcagttcg ttaatgccga ctttgccgcg
    27001 agttttcgcg tcaactttct taacgataac cgcacagtag aggctgtatt tgccatcttt
    27061 cgacggcaga ttacctgaaa caaccacaga ccccgccgga acgcgaccgt agtggatttc
    27121 gccggtttca cggtcgtaaa tacgggtgct ctgaccaatg tatacgccca tagagatgac
    27181 ggaaccttct tcgacaatca ccccttcaac cacttcggag cgtgcgccaa tgaagcagtt
    27241 atcttcaata atggtcgggt tagcctgcag cggttccagc acaccgccga tgcccacgcc
    27301 accggaaagg tggacgtttt taccaatctg cgcacaagaa ccgacggtcg cccaggtatc
    27361 aaccatggtg ccttcatcaa catatgcgcc gatgttgacg taagacggca tcagcacggt
    27421 gttacgggca ataaatgcac cctgacgtac cgccgctggt ggcacaacgc ggaagccttc
    27481 tttctgaaaa cgcgcttcat cgtagtcggc gaatttcatc ggcactttat cgaagtagcg
    27541 gctttctgcc ccttcgatca cctgattatc attaatacgg aaagagagca gcaccgcttt
    27601 tttcaaccac tgatgcgtca cccactgacc gtcaattttt tccgctacac gcagtgcacc
    27661 ggaatccagc agggcgatca cctgatttac cgcttcgcgg gtaacggtgt ctgcgttggc
    27721 tggcgtgatc tcggcacggc gttcaaaagc ggtttcaata atgttctgta actgctgcat
    27781 tgttaaactc ttttcatatc agtaaacaca tcacccttta tcgtttggat tgagggcctc
    27841 tgtcaaccgc tgatgcactt cctgctgcaa ctcgttatta agtgcacgcc ggtcagcggt
    27901 ggcaattatg aataaatctt ctactcgctc gccaatggtt gtaattcggg caccatgaag
    27961 cgaaattccc agatcggcaa aaattttccc gactctcgcc agcagtcctg gttggtcgag
    28021 cgcgataagc tcaaggaacg atttgcggtc ggtatgggtt ggcaaaaacg tcacttcggt
    28081 ttcaacagta aagtgacgta gtttagccgg ttgacgacgc ggctgtggtg gctgccagct
    28141 actttgtgtc agtacctgct ccagaccaaa ccgaataacc tcatgacgat ctgcggatag
    28201 cgggctacca tcgggttcca gcacgatgaa ggtatccatc gccataccgt cgcgagtggt
    28261 gaaaatttgt gcgtcgtgaa cacttaaatt gcggcggtct aattcggcac agacggcggc
    28321 aaacagatag gggcggtccg ggctccagat aaaaatctcg gtgcctccac gcgtagcctg
    28381 cgggctaagc aataccagcg gtttgcttaa atcatgctgt aataaatggc gggcatgcca
    28441 ggccagttga tttgggctat ggcggacaaa atagttagca cgacagcgtg accaaatttg
    28501 gtgcagcgcc tcttcgtcga tgttatccat gcgcagtagt gccagtgcct ggagttggtg
    28561 atggcgaacc cgttcgcgca tatccggcgt gttttgcatt ccgcgtcgta gctgtttttc
    28621 ggtggcaaaa tagagttcgc gcaacagact ttgcttccag ctattccaca gcgtttcgtt
    28681 ggtggcgcaa atgtcagcca ctgtcaggca taccagataa cgaagtcggt tttccgtttg
    28741 aacttcttcg gcaaactgct tgatgacttc agggtcctga atatcgcggc gttgtgctgt
    28801 caccgacatc agcaagtgct ggcgaaccag ccaggcaacc agctgtgttt cgcgtgagtt
    28861 cagcccgtgg agttctgcaa aatgcactac atcctgagca ccgagaatgg agtggtcgcc
    28921 gccgcgtcct ttggcgatat cgtgaaacag cgcggcgatg aaaatcagct cagttgacgg
    28981 caggcgcggc cagacgtcca cacacaacgg atggcgctgg cgcgtttctt cactggcaaa
    29041 actctccagt ttcagcatca cgcggatagt atgttcatcc accgtgtagg cgtggaacag
    29101 atcaaactgc atctgcccga cgatatgcga ccattgcggc atatacgcgc cgagcacgct
    29161 atggcgatgc attggcaata gcccgcgccg caccgctccg gggtgacgca gaatgctcaa
    29221 aaacagtttt cgtgcttccg gaatattaca cagcggttgt tgcagatggc gacgggcatg
    29281 gcgtaactgg cgcagcgtgg tggagtaaat gccggtgatc gcactgttgc gcaccatggt
    29341 gtagaacata cgcaagatgg cttccggctg gcgcataaat agtgtttcat cacgcaggtc
    29401 gattagcgta ccgcgtagct gaaactcatc gtcgattgga cgtggttttt cgtcggcggg
    29461 aagggcgagg atggcttcat cgaacagttg cagcagcatc tggttgagtt cactgacgcg
    29521 gcgtgtaacg cggaagtaat ccttcatcat ccgctcgacc ggttcgttgc cttcaccact
    29581 gtaattcaga cgctgagcga cgctaagttg gcgatcgaat aacagacgat tatcgtaacg
    29641 gctgacgacc agatgcaggg caaagcgaat acgccacagt atatgcagac attcgtttaa
    29701 ttccgcccgc tccgctgagg ttaagaagcc aaacccgacc atttcatcca gcgatgttgc
    29761 gccaaaatga cggcgggcca cccattgcag agtgtgaata tcgcgcaagc cgccagggct
    29821 acttttgatg tctggttcaa ggttgtagct ggtgccatgg taacgctgat ggcgctggtt
    29881 ctgttcttca actttcgccg cgtagaactt gtcggaaggc cagaatcctt cgctaaaaat
    29941 atgtttttgc agttcgagga agagcgcaac atcgccaatt aataagcggg attcgattaa
    30001 attggtggcg acggttaaat ccgataaccc ttccagcatg cactcttcaa gcgtgcgcac
    30061 actatgaccg acttccagct ttacatccca gagcagcgtt aacagctcgc ccactttttg
    30121 cgcctgatcg tccgggagct ttttacggct taaaatcagt aaatcgatgt cagaaagtgg
    30181 atgcagctcg ccacgaccat agccaccgac ggcgaccaat gccaggtcgg caatctggct
    30241 gaatcccgct tcaatccata atcgttgcag gagctggtcg ataaactcgg tgcgcgcctc
    30301 aatcaactgt tctgcagaga tcccattgtc aaaggcatca cccagccaac gctggaaagt
    30361 atcgatatgg gcttttatcc caccgacggt taattcatca cggggccaga cgcatggatt
    30421 ttgcggttga ccgggcaggg tggggagagc ggtgtttgcg tactgttctg gaagggtatt
    30481 cattgtgcgc cacccataag attaaattat cgcattaaaa aagccggcat tcgccggctt
    30541 catcttattc gtcgtgcgag attatcgccg ggatggtgtc atccttgcgt agcgtcaaaa
    30601 tttcgcagcc gttatcagtc accacaatag tatgctcata ttgtgcagac aagctgcgat
    30661 ctttggtttt taccgtccag ccatctttca tggtgcggat ctctttttta cccgcgttga
    30721 ccattggctc aatggtgaac gtcatccccg gtttcagcac gacgttggtt tcacgggagt
    30781 catagtgcag cacctgcggt tcttcatgga agccgcgacc aataccgtgt ccacaatatt
    30841 cacgaacgac ggagaagcct tctgcttcga caaacttctg aatcgccgca ccgatttcgc
    30901 gcaggttaat gcctggtttg accatgcgca gcgccaggta caggctttct tgcgtgatgc
    30961 ggcacagacg ttcgcccatg atggtcggct taccgacgat aaacattttc gaggtatcgc
    31021 cgtggaaacc atctttgatt acggtgacat caatgttaac gatatcgcca tctttcagca
    31081 gcttagcatc gtccgggata ccgtggcaca ccacttcatt aatagagatg caaacggatt
    31141 tcggatagcc gtgatagccg aggcaggcag aaaccgcgtg ttgttcatta acaatgtaat
    31201 cattacagat gcgatccagc tcgccggtgc tgacgcccgg tttaacatac ggttcgatca
    31261 tctccagcac ttcggcagcc agtcggccag cgacgcgcat tttttcgata tcttctgggg
    31321 tcttgattga gatagccatt aattctgtcc atcagcgtcg gtgataccga caatatatat
    31381 gtaagtgccg tcaatggtat cacacccggg caaattgaga atcattctga atttcgccaa
    31441 acgtgccact gaaggttttc tataatagaa aattcgacat ctgatgctgt acacagcgcc
    31501 aacaattatt ggtgtccatg acgtatttgt ggtataaagc gcgccggact tccgatccat
    31561 ttcgtataca cagactggac ggaagcgaca atctcacttt gtgtaacaac acacacgtat
    31621 cggcacatat tccggggtgc cctttggggt cggtaatatg ggatacgtgg aggcataacc
    31681 ccaactttta tatagaggtt ttaatcatgg caactgtttc catgcgcgac atgctcaagg
    31741 ctggtgttca cttcggtcac cagacccgtt actggaaccc gaaaatgaag ccgttcatct
    31801 tcggtgcgcg taacaaagtt cacatcatca accttgagaa aactgtaccg atgttcaacg
    31861 aagctctggc tgaactgaac aagattgctt ctcgcaaagg taaaatcctt ttcgttggta
    31921 ctaaacgcgc tgcaagcgaa gcggtgaaag acgctgctct gagctgcgac cagttcttcg
    31981 tgaaccatcg ctggctgggc ggtatgctga ctaactggaa aaccgttcgt cagtccatca
    32041 aacgtctgaa agacctggaa actcagtctc aggacggtac tttcgaaaag ctgaccaaga
    32101 aagaagcgct gatgcgcact cgtgagctgg agaaactgga aaacagcctg ggcggtatca
    32161 aagacatggg cggtctgccg gacgctctgt ttgtaatcga tgctgaccac gaacacattg
    32221 ctatcaaaga agcaaacaac ctgggtattc cggtatttgc tatcgttgat accaactctg
    32281 atccggacgg tgttgacttc gttatcccgg gtaacgacga cgcaatccgt gctgtgaccc
    32341 tgtacctggg cgctgttgct gcaaccgtac gtgaaggccg ttctcaggat ctggcttccc
    32401 aggcggaaga aagcttcgta gaagctgagt aataaggctt gataactccc ccaaaatagt
    32461 tcgagttgca gaaaggcggc aagctcgaga attcccggga gcttacatca gtaagtgacc
    32521 gggatgagcg agcgaagata acgcatctgc ggcgcaaaat atgaaggggg agagccctta
    32581 tagaccaggt agtacacgtt tggttagggg gcctgcatat ggcccccttt ttcactttta
    32641 tatctgtgcg gtttaatgcc gggcagatca catctccgag gattttagaa tggctgaaat
    32701 taccgcatcc ctggtaaaag agctgcgtga gcgtactggc gcaggcatga tggattgcaa
    32761 aaaagcactg actgaagcta acggcgacat cgagctggca atcgaaaaca tgcgtaagtc
    32821 cggtgctatt aaagcagcga aaaaagcagg caacgttgct gctgacggcg tgatcaaaac
    32881 caaaatcgac ggcaactacg gcatcattct ggaagttaac tgccagactg acttcgttgc
    32941 aaaagacgct ggtttccagg cgttcgcaga caaagttctg gacgcagctg ttgctggcaa
    33001 aatcactgac gttgaagttc tgaaagcaca gttcgaagaa gaacgtgttg cgctggtagc
    33061 gaaaattggt gagaacatca acattcgccg cgttgctgcg ctggaaggcg acgttctggg
    33121 ttcttatcag cacggtgcgc gtatcggcgt tctggttgct gctaaaggcg ctgacgaaga
    33181 gctggttaaa cacatcgcta tgcacgttgc tgcaagcaag ccagaattca tcaaaccaga
    33241 agacgtatcc gctgaagtgg tagaaaaaga ataccaggta cagctggata tcgcgatgca
    33301 gtctggtaag ccgaaagaaa tcgcagagaa aatggttgaa ggccgcatga agaaattcac
    33361 cggcgaagtt tctctgaccg gtcagccgtt cgttatggaa ccaagcaaaa ctgttggtca
    33421 gctgctgaaa gagcataacg ctgaagtgac tggcttcatc cgcttcgaag tgggtgaagg
    33481 catcgagaaa gttgagactg actttgcagc agaagttgct gcgatgtcca agcagtctta
    33541 attatcaaaa aggagccgcc tgagggcggc ttctttttgt gcccatcttg taaattcagc
    33601 taacccttgt ggggctgcgc tgaaaagcga cgtacaatgt cgctagtatt aattcatttc
    33661 aatcgttgac agtctcagga aagaaacatg gctaccaatg caaaacccgt ctataaacgc
    33721 attctgctta agttgagtgg cgaagctctg cagggcactg aaggcttcgg tattgatgca
    33781 agcatactgg atcgtatggc tcaggaaatc aaagaactgg ttgaactggg tattcaggtt
    33841 ggtgtggtga ttggtggggg taacctgttc cgtggcgctg gtctggcgaa agcgggtatg
    33901 aaccgcgttg tgggcgacca catggggatg ctggcgaccg taatgaacgg cctggcaatg
    33961 cgtgatgcac tgcaccgcgc ctatgtgaac gctcgtctga tgtccgctat tccattgaat
    34021 ggcgtgtgcg acagctacag ctgggcagaa gctatcagcc tgttgcgcaa caaccgtgtg
    34081 gtgatcctct ccgccggtac aggtaacccg ttctttacca ccgactcagc agcttgcctg
    34141 cgtggtatcg aaattgaagc cgatgtggtg ctgaaagcaa ccaaagttga cggcgtgttt
    34201 accgctgatc cggcgaaaga tccaaccgca accatgtacg agcaactgac ttacagcgaa
    34261 gtgctggaaa aagagctgaa agtcatggac ctggcggcct tcacgctggc tcgtgaccat
    34321 aaattaccga ttcgtgtttt caacatgaac aaaccggggg cgctgcgccg tgtggtaatg
    34381 ggtgaaaaag aagggacttt aatcacggaa taattcccgt gatggataaa taagggtaag
    34441 attccgcgta agtatcgcgg gggcgttagt ctggttataa ggcgttattg ttgcaggcag
    34501 tttggtcacg gccagcgcgc agcaaccgga gcgtacaaaa gtacgtgagg atggcgagca
    34561 ctgcccgggg ccaaaatggc aaataaaata gcctaataat ccagacgatt acccgtaata
    34621 tgtttaatca gggctatact tagcacactt ccactgtgtg tgactgtctg gtctgactga
    34681 gacaagtttt caaggattcg taacgtgatt agcgatatca gaaaagatgc tgaagtacgc
    34741 atggacaaat gcgtagaagc gttcaaaacc caaatcagca aaatacgcac gggtcgtgct
    34801 tctcccagcc tgctggatgg cattgtcgtg gaatattacg gcacgccgac gccgctgcgt
    34861 cagctggcaa gcgtaacggt agaagattcc cgtacactga aaatcaacgt gtttgatcgt
    34921 tcaatgtctc cggccgttga aaaagcgatt atggcgtccg atcttggcct gaacccgaac
    34981 tctgcgggta gcgacatccg tgttccgctg ccgccgctga cggaagaacg tcgtaaagat
    35041 ctgaccaaaa tcgttcgtgg tgaagcagaa caagcgcgtg ttgcagtacg taacgtgcgt
    35101 cgtgacgcga acgacaaagt gaaagcactg ttgaaagata aagagatcag cgaagacgac
    35161 gatcgccgtt ctcaggacga tgtacagaaa ctgactgatg ctgcaatcaa gaaaattgaa
    35221 gcggcgctgg cagacaaaga agcagaactg atgcagttct gatttcttga acgacaaaaa
    35281 cgccgctcag tagatccttg cggatcggct ggcggcgttt tgctttttat tctgtctcaa
    35341 ctctggatgt ttcatgaagc aactcaccat tctgggctcg accggctcga ttggttgcag
    35401 cacgctggac gtggtgcgcc ataatcccga acacttccgc gtagttgcgc tggtggcagg
    35461 caaaaatgtc actcgcatgg tagaacagtg cctggaattc tctccccgct atgccgtaat
    35521 ggacgatgaa gcgagtgcga aacttcttaa aacgatgcta cagcaacagg gtagccgcac
    35581 cgaagtctta agtgggcaac aagccgcttg cgatatggca gcgcttgagg atgttgatca
    35641 ggtgatggca gccattgttg gcgctgctgg gctgttacct acgcttgctg cgatccgcgc
    35701 gggtaaaacc attttgctgg ccaataaaga atcactggtt acctgcggac gtctgtttat
    35761 ggacgccgta aagcagagca aagcgcaatt gttaccggtc gatagcgaac ataacgccat
    35821 ttttcagagt ttaccgcaac ctatccagca taatctggga tacgctgacc ttgagcaaaa
    35881 tggcgtggtg tccattttac ttaccgggtc tggtggccct ttccgtgaga cgccattgcg
    35941 cgatttggca acaatgacgc cggatcaagc ctgccgtcat ccgaactggt cgatggggcg
    36001 taaaatttct gtcgattcgg ctaccatgat gaacaaaggt ctggaataca ttgaagcgcg
    36061 ttggctgttt aacgccagcg ccagccagat ggaagtgctg attcacccgc agtcagtgat
    36121 tcactcaatg gtgcgctatc aggacggcag tgttctggcg cagctggggg aaccggatat
    36181 gcgtacgcca attgcccaca ccatggcatg gccgaatcgc gtgaactctg gcgtgaagcc
    36241 gctcgatttt tgcaaactaa gtgcgttgac atttgccgca ccggattatg atcgttatcc
    36301 atgcctgaaa ctggcgatgg aggcgttcga acaaggtcag gcagcgacga cagcattgaa
    36361 tgccgcaaac gaaatcaccg ttgctgcttt tcttgcgcaa caaatccgct ttacggatat
    36421 cgctgcgttg aatttatccg tactggaaaa aatggatatg cgcgaaccac aatgtgtgga
    36481 cgatgtgtta tctgttgatg cgaacgcgcg tgaagtcgcc agaaaagagg tgatgcgtct
    36541 cgcaagctga ggataatccg gctacagaga gtcgcgctat ttgttagcgt agggcttcag
    36601 tgatatagtc tgcgccatct gatcgtaagt agttggcttt ataaggtcag gtatgccgtg
    36661 gttttacacg gctttttttt gtataggctt cagtattcct gagtaccgta aaccctgtca
    36721 gggaataaaa aacgcgtgat gttgtctgct actcaaccac ttagcgaaaa attgccagcg
    36781 catggctgcc gtcatgttgc gatcattatg gacggcaatg gtcgctgggc aaaaaagcaa
    36841 gggaagattc gtgcctttgg gcataaagcc ggggcaaaat ccgtccgccg ggctgtctct
    36901 tttgcggcca acaacggtat tgaggcgtta acgctgtatg cctttagtag tgaaaactgg
    36961 aaccgaccag cgcaggaagt cagtgcgtta atggaactgt ttgtgtgggc gctcgatagc
    37021 gaagtaaaaa gtctgcaccg acataacgtg cgtctgcgta ttattggcga taccagtcgc
    37081 tttaactcgc gtttgcaaga acgtattcgt aaatctgaag cgctaacagc cgggaatacc
    37141 ggtctgacgc tgaatattgc ggcgaactac ggtggacgtt gggatatagt ccagggagtc
    37201 aggcaactgg ctgaaaaggt gcagcaagga aacctgcaac cagatcagat agatgaagag
    37261 atgctaaacc agcatgtctg tatgcatgaa ctggcccctg tagatttagt aattaggact
    37321 gggggggagc atcgcattag taactttttg ctttggcaaa ttgcctatgc cgaactttac
    37381 tttacagatg ttctctggcc cgatttcgat gaacaagact ttgaaggggc gttaaatgcc
    37441 tttgctaatc gagagcgtcg tttcggcggc accgagcccg gtgatgaaac agcctgatgg
    37501 gggtcgcttt tgctgaagta tcgcctgata tctgcttttg tgttaatacc cgtcgtcatc
    37561 gcggcgttgt ttctgttgcc gccggtgggg ttcgccatcg taacgctggt ggtctgcatg
    37621 ctggcagcgt gggaatgggg acagcttagc ggttttacca ctcgttcgca gcgagtatgg
    37681 ttggcggtgt tatgcgggtt attgttggcg ctgatgcttt ttctgttgcc ggaatatcac
    37741 cgaaatattc atcaaccgct ggttgaaatc tcactttggg cttcgctggg ttggtggatt
    37801 gtcgcgctat tgctggtgct gttttaccca ggttccgcag caatctggcg aaactctaaa
    37861 acattgcgcc ttatttttgg cgtgctaacc attgttccct tcttctgggg catgctggcg
    37921 ttacgggcct ggcactatga cgagaatcat tacagtggcg caatatggct gctctatgtc
    37981 atgatcctgg tatggggcgc tgactctggc gcatatatgt ttggcaaatt gtttggtaaa
    38041 cataagctgg caccgaaggt ttctccgggt aaaacctggc aaggctttat aggtggacta
    38101 gcgaccgcag cggtaatctc atggggttat ggcatgtggg cgaatctcga cgtcgctccc
    38161 gtcaccttac tcatttgctc tattgtcgca gcgttagcct cagtgctcgg cgatctgacc
    38221 gagagtatgt ttaagcgtga agcaggaatt aaggacagcg gtcatttaat tccaggacac
    38281 ggtggtattt tagatcgtat tgatagcctg acggctgcgg taccggtctt tgcttgcttg
    38341 ttgttactgg tattcaggac gctttaacgg aaggtaatat gctgagtttt ctctgggatt
    38401 tggcttcgtt catcgttgca ctgggtgtac ttatcaccgt gcatgaattt ggtcatttct
    38461 gggttgcccg gcgttgtggt gttcgcgttg agcgtttctc aatagggttt ggtaaggcgc
    38521 tctggcggcg aactgataag ctcggcaccg aatatgttat cgccctgatc ccgctgggcg
    38581 gttatgtcaa aatgctggat gagcgcgcag aaccggtcgt tccggaactc cgccaccatg
    38641 ccttcaataa taaatctgtt ggccaacgag cggcgattat tgccgcaggt ccggttgcaa
    38701 acttcatttt tgctatcttt gcctactggc tggtttttat tattggtgtg cctggcgtac
    38761 gtccggtggt tggtgaaata gcagccaatt cgatagctgc ggaagcacaa attgcaccag
    38821 gtacggaact aaaagccgta gatggtatcg aaacgcctga ttgggatgcc gtgcgtttgc
    38881 agttggtcga taaaattggc gatgaaagca ccaccattac agtagcgcca tttggcagcg
    38941 atcaacggcg ggatgtaaag ctcgatttac gtcactgggc gtttgagcct gataaagaag
    39001 atccggtatc ttcgctgggg attcgtcctc gtgggccgca aattgaacct gtactggaaa
    39061 atgtgcagcc aaactcggcg gcaagcaagg caggtttgca agcaggcgac aggatcgtta
    39121 aagtcgatgg tcagccctta acgcagtggg tgacctttgt gatgcttgtc cgggataacc
    39181 cgggtaaatc cttagcgtta gaaatcgaaa ggcagggaag tcccttgtct ttgacattaa
    39241 tcccggagag taaaccgggt aatggtaaag cgattggttt tgtcggtatt gagccgaaag
    39301 tcattccttt gccagatgag tataaagttg tacgccagta tgggccgttc aacgccatcg
    39361 tcgaagccac ggacaaaacg tggcagctga tgaagctgac ggtcagtatg ctgggaaaat
    39421 tgatcaccgg tgatgtgaaa ctgaacaacc tcagtgggcc gatctctatc gccaaggggg
    39481 ctgggatgac agcggaactc ggggtagttt attacctgcc gtttcttgcg cttattagcg
    39541 tgaacttagg gataattaac ctgtttccgt tgcccgtact tgacgggggg catctgctgt
    39601 tccttgcgat cgaaaagatc aagggcggac cggtatccga gcgggttcaa gacttttgtt
    39661 atcgcattgg ctcgattctg ctggtgctgt taatggggct tgcacttttc aatgatttct
    39721 ctcggttatg agagttagtt aggaagaacg cataataacg atggcgatga aaaagttgct
    39781 catagcgtcg ctgctgttta gcagcgccac cgtatacggt gctgaagggt tcgtagtgaa
    39841 agatattcat ttcgaaggcc ttcagcgtgt cgccgttggt gcggccctcc tcagtatgcc
    39901 ggtgcgcaca ggcgacacgg ttaatgatga agatatcagt aataccattc gcgctctgtt
    39961 tgctaccggc aactttgagg atgttcgcgt ccttcgtgat ggtgataccc ttctggttca
    40021 ggtaaaagaa cgtccgacca ttgccagcat tactttctcc ggtaacaaat cggtgaaaga
    40081 tgacatgctg aagcaaaacc tcgaggcttc tggtgtgcgt gtgggcgaat ccctcgatcg
    40141 caccaccatt gccgatatcg agaaaggtct ggaagacttc tactacagcg tcggtaaata
    40201 tagcgccagc gttaaagctg tcgtgacccc gctgccgcgc aaccgtgttg acctaaaact
    40261 ggtgttccag gaaggtgtgt cagctgaaat ccagcaaatt aacattgttg gtaaccatgc
    40321 tttcaccacc gacgaactga tctctcattt ccaactgcgt gacgaagtgc cgtggtggaa
    40381 cgtggtaggc gatcgtaaat accagaaaca gaaactggcg ggcgaccttg aaaccctgcg
    40441 cagctactat ctggatcgcg gttatgcccg tttcaacatc gactctaccc aggtcagtct
    40501 gacgccagat aaaaaaggta tttacgtcac ggtgaacatt accgaaggcg atcagtacaa
    40561 gctttctggc gttgaagtga gcggcaacct tgccgggcac tccgctgaaa ttgagcagct
    40621 gactaagatc gagccgggcg agctgtataa cggcaccaaa gtgaccaaga tggaagatga
    40681 catcaaaaag cttctcggtc gctatggtta tgcctatccg cgcgtacagt cgatgcctga
    40741 aattaacgat gccgacaaaa ccgttaaatt acgcgtgaac gttgatgcgg gtaaccgttt
    40801 ctacgtgcgt aagatccgtt ttgaaggtaa cgatacctcg aaagatgccg tcctgcgtcg
    40861 cgaaatgcgt cagatggaag gtgcatggct gggaagcgat ctggtcgatc agggtaagga
    40921 gcgtctgaat cgtctgggct tctttgaaac tgtcgatacc gatacccaac gtgttccggg
    40981 tagcccggac caggtagatg tcgtctacaa ggtaaaagag cgcaacaccg gtagcttcaa
    41041 ctttggtatt ggttacggta ctgaaagtgg cgtgagcttc caggctggtg tgcagcagga
    41101 taactggtta ggtacaggtt atgctgttgg tatcaacggg accaaaaacg attaccagac
    41161 ctatgctgaa ctgtcggtaa ccaacccgta cttcactgta gatggtgtaa gcctcggtgg
    41221 tcgtctcttc tataatgact tccaggcaga tgacgccgac ctgtccgact ataccaacaa
    41281 gagttatggt acagacgtga cgttgggctt cccgatcaac gaatataact cgctgcgtgc
    41341 aggtctgggt tatgtacata actccctgtc caacatgcag cctcaggttg cgatgtggcg
    41401 ttatctgtac tctatgggtg aacatccgag cacctctgat caggataata gcttcaaaac
    41461 ggacgacttc acgttcaact atggttggac ctataacaag cttgaccgtg gttacttccc
    41521 gacagatggt tcacgtgtca acctgaccgg taaagtgacc attcctggat cggataacga
    41581 atactacaaa gtgacgttag acacggcgac ttatgtgccg atcgatgacg atcacaaatg
    41641 ggttgttctg gggcgtaccc gctggggtta tggtgatggt ttaggcggca aagagatgcc
    41701 gttctacgag aacttctatg ccggtggttc cagcaccgtg cgtggcttcc agtccaatac
    41761 aattggtccg aaagcagttt acttcccgca tcaggccagt aattatgatc cggactatga
    41821 ttacgaatgt gcgactcagg acggcgcgaa agacctgtgt aaatcggatg atgctgtagg
    41881 cggtaacgcc atggcggttg ccagcctcga gttcatcacc ccgacgccgt ttattagcga
    41941 taagtatgct aactcggttc gtacttcctt cttctgggat atgggtaccg tttgggatac
    42001 aaactgggat tccagccaat attctggata tccggactat agtgatccaa gcaatatccg
    42061 tatgtctgcg ggtatcgcat tacaatggat gtccccattg gggccgttgg tgttctccta
    42121 cgcccagccg ttcaaaaagt acgatggaga caaggcagaa cagttccagt ttaacatcgg
    42181 taaaacctgg taagtgttct ccacaaagga atgtagtggt agtgtagcga tgactttagg
    42241 cgatcaatat aagatcgccg ggccacgcaa agaactgcac cctccggtgc aaatgggatg
    42301 gtaaggagtt tattgtgaaa aagtggttat tagctgcagg tctcggttta gcactggcaa
    42361 cttctgctca ggcggctgac aaaattgcaa tcgtcaacat gggcagcctg ttccagcagg
    42421 tagcgcagaa aaccggtgtt tctaacacgc tggaaaatga gttcaaaggc cgtgccagcg
    42481 aactgcagcg tatggaaacc gatctgcagg ctaaaatgaa aaagctgcag tccatgaaag
    42541 cgggcagcga tcgcactaag ctggaaaaag acgtgatggc tcagcgccag acttttgctc
    42601 agaaagcgca ggcttttgag caggatcgcg cacgtcgttc caacgaagaa cgcggcaaac
    42661 tggttactcg tatccagact gctgtgaaat ccgttgccaa cagccaggat atcgatctgg
    42721 ttgttgatgc aaacgccgtt gcttacaaca gcagcgatgt aaaagacatc actgccgacg
    42781 tactgaaaca ggttaaataa gtaatgcctt caattcgact ggctgattta gcgcagcagt
    42841 tggatgcaga actacacggt gatggcgata tcgtcatcac cggcgttgcg tccatgcaat
    42901 ctgcacaaac aggtcacatt acgttcatgg ttaacccaaa ataccgtgag catttaggct
    42961 tgtgccaggc gtccgcggtt gtcatgaccc aggacgatct tcctttcgcg aaaagtgccg
    43021 cgctggtagt gaagaatccc tacctgactt acgcgcgcat ggcgcaaatt ttagatacca
    43081 cgccgcagcc cgcgcagaac attgcaccca gtgcggtgat cgacgcgacg gcgaagctgg
    43141 gtaacaacgt atcgattggc gctaacgcgg tgattgagtc cggcgttgaa ctgggcgata
    43201 acgtgattat cggtgccggt tgcttcgtag gtaaaaacag caaaatcggt gcaggttcgc
    43261 gtctctgggc gaacgtaacc atttaccatg agatccagat cggtcagaat tgcctgatcc
    43321 agtccggaac agtggtaggc gcagacggct ttggttatgc caacgatcgt ggtaactggg
    43381 tgaagatccc acagattggt cgcgtaatta ttggcgatcg cgtggagatc ggtgcctgca
    43441 caaccatcga tcgcggcgcg ctggatgaca ctattattgg caatggcgtg atcattgata
    43501 accagtgcca gattgcacat aacgtcgtga ttggcgacaa tacggcggtt gccggtggcg
    43561 tcattatggc gggcagcctg aaaattggtc gttactgcat gatcggcgga gccagcgtaa
    43621 tcaacgggca tatggaaata tgcgacaaag tgacggttac gggcatgggt atggtgatgc
    43681 gtcccatcac tgaaccaggc gtctattcct caggcattcc gctgcaaccc aacaaagtct
    43741 ggcgcaaaac cgctgcactg gtgatgaaca ttgatgacat gagcaagcgt ctgaaatcgc
    43801 ttgagcgcaa ggttaatcaa caagactaac gttccatctt ttgttcgcca aactttacgg
    43861 cctgtctcat tcttacgatt gcggcaggcc gtgttattat tgtcgtttct tatattttga
    43921 caggaagagt atcttgacta ctaacactca tactctgcag attgaagaga ttttagaact
    43981 tctgccgcac cgtttcccgt tcttactggt ggatcgcgtg ctggattttg aagaaggtcg
    44041 ttttctgcgc gcagtaaaaa atgtctctgt caatgagcca ttcttccagg gccatttccc
    44101 tggaaaaccg attttcccgg gtgtgctgat tctggaagca atggcacagg caacaggtat
    44161 tctggcgttt aaaagcgtag gaaaactgga accgggtgaa ctgtactact tcgctggtat
    44221 tgacgaagcg cgcttcaagc gcccggtcgt gcctggcgat caaatgatca tggaagtcac
    44281 tttcgaaaaa acgcgccgcg gcctgacccg ttttaaaggg gttgctctgg tcgatggtaa
    44341 agtagtttgc gaagcaacga tgatgtgtgc tcgtagccgg gaggcctgat acgtgattga
    44401 taaatccgcc tttgtgcatc caaccgccat tgtggaagag ggcgcgtcaa ttggcgcgaa
    44461 cgcacacatt ggtccttttt gtatcgttgg accccatgtc gaaattggtg agggtaccgt
    44521 actgaaatct cacgttgtcg tgaatggtca tactaaaatt ggccgcgata atgagattta
    44581 tcagttcgcc tccatcggcg aagttaacca ggatttgaaa tatgctggcg aaccgacccg
    44641 tgtggaaatc ggcgatcgta accgcattcg cgaaagcgtc accattcatc gtggcacagt
    44701 ccagggcggt ggattgacga aggtgggcag cgacaactta ctgatgatca acgcgcacat
    44761 tgcgcacgat tgtacggtag gtaaccgctg tattctcgcc aacaacgcaa cgctggcggg
    44821 tcacgtatcg gttgacgact tcgcgatcat cggcggcatg accgcagtcc atcagttctg
    44881 catcattggt gcgcatgtga tggttggcgg ctgctccggt gtggcgcagg acgtccctcc
    44941 ttatgttatt gcgcagggta accacgcaac gccgttcggt gtcaatatcg aagggctgaa
    45001 gcgccgcgga ttcagccgtg aggcgattac cgctatccgc aatgcgtata agctgattta
    45061 tcgtagcggt aaaacgctcg atgaagtgaa accggaaatt gctgaactgg cggaaacata
    45121 tccggaagtg aaagccttta ccgatttctt tgcacgctca acgcgcggtc tgattcgtta
    45181 atgactgacc agcgttcatt aacgattgcc ctggtcgccg gagaaacctc cggcgatatc
    45241 ctgggggccg gtttaatccg cgctctgaaa gaacgtgtgc ccaacgcccg ctttgttggt
    45301 gttgccgggc cacgaatgca ggctgaaggc tgcgaagcct ggtacgaaat ggaagaactg
    45361 gcggtgatgg gcattgttga agtgctcggt cgtctgcgtc gcttactgca tattcgtgcc
    45421 gatctgacaa agcgttttgg cgaactgaag ccagatgttt ttgttggtat tgatgcgccc
    45481 gacttcaata ttactcttga aggtaacctc aaaaagcagg gtatcaaaac cattcattac
    45541 gtcagtccat ccgtctgggc gtggcgacag aaacgcgttt tcaaaatagg cagagccacc
    45601 gatctggtgc tcgcatttct gcctttcgaa aaagcgtttt atgacaaata caatgtaccg
    45661 tgccgcttta tcggtcatac catggctgat gccatgcctt tagatccaga taaaaatgcc
    45721 gcccgtgatg tgctggggat ccctcacgat gcccactgcc tggcgttgct accggggagc
    45781 cgtggtgcag aagtcgaaat gcttagcgcc gatttcctga aaactgccca gcttttgcgc
    45841 cagacatatc cggatctcga aatcgtggtg ccgctggtga atgccaaacg ccgcgagcag
    45901 tttgaacgca tcaaagctga agtcgcgcca gacctgtcgg ttcatttgct ggatggaatg
    45961 ggccgtgagg cgatggtcgc cagcgatgcg gcactactgg cgtcggggac ggcagccctg
    46021 gagtgtatgc tggcgaaatg cccgatggtg gtgggatatc gcatgaagcc tttcaccttc
    46081 tggctggcga agcgactggt gaaaactgat tatgtctcgc tgccaaatct gctggcgggc
    46141 agagagttag tcaaagagtt attgcaggaa gagtgtgagc cgcaaaaact ggctgcggcg
    46201 ctgttaccgc tgttggcgaa cgggaaaacc agccacgcga tgcacgatac cttccgtgaa
    46261 ctgcatcagc agatccgctg caatgccgat gagcaggcgg cacaagccgt tctggagtta
    46321 gcacaatgat cgaatttgtt tatccgcaca cgcagctggt tgcgggtgtg gatgaagtcg
    46381 gacgcgggcc gttagttggc gcggtcgtca ccgctgcggt gatccttgac ccggcgcgcc
    46441 cgattgccgg gctgaatgat tccaaaaagc tgagcgaaaa acgtcgtctg gcgctctgtg
    46501 aagagatcaa agagaaagcg ttgagctgga gtctgggccg cgcggaacct cacgaaatcg
    46561 acgagctgaa cattcttcat gcgaccatgc tggcgatgca gcgtgccgtc gctgggctgc
    46621 atattgcgcc ggaatatgtg ttgattgatg gtaaccgctg cccgaaatta ccgatgcctg
    46681 cgatggctgt ggtgaaaggc gatagccgcg taccggaaat cagtgccgcg tctatcctgg
    46741 cgaaagtgac gcgtgacgcc gaaatggcgg cgctggatat tgttttcccg caatatggtt
    46801 ttgcccaaca caaagggtac ccaaccgctt ttcatctgga aaaactggct gaacacggcg
    46861 cgaccgaaca ccatcggcgc agctttgggc ctgtcaaacg cgcactggga cttgcgtcct
    46921 gattcttgtg tcgagattaa gtaaaccgga atctgaagat gtctgaacca cgtttcgtac
    46981 acctgcgggt gcacagcgac tactcgatga tcgatggcct ggccaaaacc gcaccgttgg
    47041 taaaaaaggc ggcggcgttg ggtatgccag cactggcgat caccgatttc accaaccttt
    47101 gtggtctggt gaagttctac ggagcgggac atggcgcagg gattaagcct atcgtcgggg
    47161 cagattttaa cgtccagtgc gacctgctgg gtgatgagtt aacccacctg acggtactgg
    47221 cggcgaacaa taccggctat cagaatctga cgttgctgat ctcaaaagcg tatcagcgcg
    47281 ggtacggtgc cgccgggccg atcatcgatc gcgactggct tatcgaatta aacgaagggt
    47341 tgatccttct ttccggcgga cgcatgggcg acgtcggacg cagtcttttg cgtggtaaca
    47401 gcgcgctggt agatgagtgt gtcgcgtttt atgaagaaca cttcccggat cgctattttc
    47461 tcgagctgat ccgcaccggc aggccggatg aagaaagcta tctgcacgcg gcggtggaac
    47521 tggcggaagc gcgcggtttg cccgtcgtgg cgaccaacga cgtgcgcttt atcgacagca
    47581 gcgactttga cgcacacgaa atccgcgtcg cgatccacga cggctttacc ctcgacgatc
    47641 ctaaacgccc gcgtaactat tcgccgcagc aatatatgcg tagcgaagag gagatgtgtg
    47701 agctgtttgc cgacatcccc gaagcccttg ccaacaccgt tgagatcgcc aaacgctgta
    47761 acgtaaccgt gcgtcttggt gaatacttcc tgccgcagtt cccgaccggg gacatgagca
    47821 ccgaagatta tctggtcaag cgtgcaaaag agggcctgga agagcgtctg gcctttttat
    47881 tccctgacga ggaagaacgt gttaagcgcc gcccggaata tgacgaacgt ctggagactg
    47941 aacttcaggt tatcaaccag atgggcttcc cgggctactt cctcatcgtt atggaattta
    48001 tccagtggtc gaaagataac ggcgtaccgg tagggccagg ccgtggctcc ggtgcgggtt
    48061 cactggtggc ctacgcgctg aaaatcaccg acctcgatcc gctggaattt gacctgctgt
    48121 tcgaacgttt ccttaacccg gaacgtgtct ccatgcctga cttcgacgtt gacttctgta
    48181 tggagaaacg cgatcaggtt atcgagcacg tagcggacat gtacggtcgt gatgcggtat
    48241 cgcagatcat caccttcggt acaatggcgg cgaaagcggt gatccgcgac gtaggccgcg
    48301 tgctggggca tccgtacggc tttgtcgatc gtatctcgaa actgatcccg cccgatccgg
    48361 ggatgacgct ggcgaaagcg tttgaagccg agccgcagct gccggaaatc tacgaagcgg
    48421 atgaagaagt taaggcgctg atcgacatgg cgcgcaaact ggaaggggtc acccgtaacg
    48481 ccggtaagca cgccggtggg gtggttatcg caccgaccaa aattaccgat tttgcgccgc
    48541 tttattgcga tgaagagggc aaacatccgg tcacccagtt tgataaaagc gacgttgaat
    48601 acgccggact ggtgaagttc gacttccttg gtttgcgtac gctcaccatc atcaactggg
    48661 cgctggagat gatcaacaag cggcgggcga agaatggcga gccgccgctg gatatcgccg
    48721 cgatcccgct ggatgacaag aaaagcttcg acatgctgca acgctcggaa accactgcgg
    48781 tattccagct tgaatcgcgc ggcatgaagg acctgatcaa gcgtctgcaa cctgactgct
    48841 tcgaagatat gatcgccctg gtggcactgt tccgccccgg tccgttgcaa tcagggatgg
    48901 tggataactt tatcgaccgt aaacatggtc gtgaagagat ctcctatccg gacgtgcagt
    48961 ggcagcatga aagcttgaaa ccggtactgg agccaaccta cggcatcatc ctgtatcagg
    49021 aacaggtcat gcagattgcc caggtgcttt ctggttatac cctcggtggc gcggatatgc
    49081 tgcgtcgtgc gatgggtaag aaaaagccgg aagagatggc taagcagcgt tctgtatttg
    49141 ctgaaggtgc agaaaagaac ggaatcaacg ccgaactggc gatgaaaatc ttcgacctgg
    49201 tggagaaatt cgcgggttac ggatttaaca aatcgcactc tgcggcctat gctttggtgt
    49261 catatcaaac gttatggctg aaagcacact atcctgcgga gtttatggcg gcggtaatga
    49321 ccgccgatat ggacaacacc gagaaggtgg tgggcctggt ggatgagtgc tggcggatgg
    49381 ggctgaaaat cctgccacca gatataaact ccggtcttta ccatttccac gtcaacgacg
    49441 acggcgaaat cgtgtatggt attggcgcca tcaaaggggt aggtgaaggt ccgattgagg
    49501 ccatcatcga agcccgtaat aaaggcggct acttccgcga actgtttgat ctttgcgccc
    49561 gtaccgacac caaaaagtta aaccggcggg tgctggaaaa actgatcatg tccggggcgt
    49621 ttgaccgtct tggaccacac cgcgcggcac tgatgaactc gctgggcgat gcgttaaaag
    49681 cggcagatca acacgcgaaa gcggaagcta tcggtcaggc cgatatgttc ggcgtgctgg
    49741 ccgaagagcc ggaacaaatt gaacaatcct acgccagctg ccaaccgtgg ccggagcagg
    49801 tggtattaga tggggaacgt gaaacgttag gcctgtacct gaccggacac cctatcaacc
    49861 agtatttaaa agagattgag cgttatgtcg gaggcgtaag gctgaaagac atgcacccga
    49921 cagaacgtgg taaagtcatc acggctgcgg ggctcgttgt tgccgcgcgg gttatggtca
    49981 ccaagcgcgg caatcgtatc ggtatctgca cgctggatga ccgttccggg cggctggaag
    50041 tgatgttgtt tactgacgcc ctggataaat accagcaatt gctggaaaaa gaccgcatac
    50101 ttatcgtcag cggacaggtc agctttgatg acttcagcgg tgggcttaaa atgaccgctc
    50161 gcgaagtgat ggatattgac gaagcccggg aaaaatatgc tcgcgggctt gctatctcgc
    50221 tgacggacag gcaaattgat gaccagcttt taaaccgact ccgtcagtct ctggaacccc
    50281 accgctctgg gacaattcca gtacatctct actatcagag ggcggatgca cgcgcgcggt
    50341 tgcgttttgg cgcgacgtgg cgtgtctctc cgagcgatcg tttattaaac gatctccgtg
    50401 gcctcattgg ttcggagcag gtggaactgg agtttgacta atacaggaat actatgagtc
    50461 tgaatttcct tgattttgaa cagccgattg cagagctgga agcgaaaatc gattctctga
    50521 ctgcggttag ccgtcaggat gagaaactgg atattaacat cgatgaagaa gtgcatcgtc
    50581 tgcgtgaaaa aagcgtagaa ctgacgcgta aaatcttcgc cgatctcggt gcatggcaga
    50641 ttgcgcaact ggcacgccat ccacagcgtc cttataccct ggattacgtt cgcctggcat
    50701 ttgatgaatt tgacgaactg gctggcgacc gcgcgtatgc agacgataaa gctatcgtcg
    50761 gtggtatcgc ccgtctcgat ggtcgtccgg tgatgatcat tggtcatcaa aaaggtcgtg
    50821 aaaccaaaga aaaaattcgc cgtaactttg gtatgccagc gccagaaggt taccgcaaag
    50881 cactgcgtct gatgcaaatg gctgaacgct ttaagatgcc aatcatcacc tttatcgaca
    50941 ccccgggggc ttatccgggc gtgggcgcag aagagcgtgg tcagtctgaa gccattgcac
    51001 gcaacctgcg tgaaatgtct cgcctcggcg taccggtagt ttgtacggtt atcggtgaag
    51061 gtggttctgg cggtgcgctg gcgattggcg tgggcgataa agtgaatatg ctgcaataca
    51121 gcacctattc cgttatctcg ccggaaggtt gtgcgtccat tctgtggaag agcgccgaca
    51181 aagcgccgct ggcggctgaa gcgatgggta tcatcgctcc gcgtctgaaa gagctgaaac
    51241 tgatcgactc catcatcccg gaaccgctgg gcggcgctca ccgtaacccg gaagcgatgg
    51301 cggcatcgtt gaaagcgcaa ctgctggcgg atctggccga tctcgatgtg ttaagcactg
    51361 aagatttaaa aaatcgtcgt tatcagcgcc tgatgagcta cggttacgcg taattcgcaa
    51421 aagttctgaa aaagggtcac ttcggtggcc cttttttatc gccacggttt gagcaggcta
    51481 tgattaagga aggattttcc aggaggaaca catgaacatc attgccatta tgggaccgca
    51541 tggcgtcttt tataaagatg agcccatcaa agaactggag tcggcgctgg tggcgcaagg
    51601 ctttcagatt atctggccac aaaacagcgt tgatttgctg aaatttatcg aacataaccc
    51661 tcgaatttgc ggcgtgattt ttgactggga tgagtacagt ctcgatttat gcagcgatat
    51721 caatcaactt aatgaatatc tcccgcttta tgccttcatc aacacccact cgacgatgga
    51781 tgtcagcgtg caggatatgc ggatggcgct ctggtttttt gaatatgcgc tggggcaggc
    51841 ggaagatatc gccattcgta tgcgtcagta caccgacgaa tatcttgata acattacacc
    51901 gccgttcacg aaagccttgt ttacctacgt caaagagcgg aagtacacct tttgtacgcc
    51961 ggggcatatg ggcggcaccg catatcaaaa aagcccggtt ggctgcctgt tttatgattt
    52021 tttcggcggg aataccctca aggctgatgt ctctatttcg gtcaccgagc ttggttcgtt
    52081 gctcgaccac accgggccac acctggaagc ggaagagtac atcgcgcgga cttttggcgc
    52141 ggaacagagt tatatcgtta ccaacggaac atcgacgtcg aacaaaattg tgggtatgta
    52201 cgccgcgcca tccggcagta cgctgttgat cgaccgcaat tgtcataaat cgctggcgca
    52261 tctgttgatg atgaacgatg tagtgccagt ctggctgaaa ccgacgcgta atgcgttggg
    52321 gattcttggt gggatcccgc gccgtgaatt tactcgcgac agcatcgaag agaaagtcgc
    52381 tgccaccacg caggcacaat ggccggttca tgcggtgatt accaactcca cctatgatgg
    52441 cttgctctac aacaccgact ggatcaaaca gacgctggat gtcccgtcga tccacttcga
    52501 ttccgcctgg gtgccgtaca cccattttca tccgatctac caggggaaaa gtggtatgag
    52561 cggcgagcgt gttgcgggaa aagtgatctt cgaaacgcaa tcgacccaca aaatgctggc
    52621 ggcgttatcg caggcttcgc tgatccacat taaaggcgag tatgacgaag aggcgtttaa
    52681 cgaagccttt atgatgcata ccaccacctc gcccagttat cccattgttg cttcggttga
    52741 gacggcggcg gcgatgctgc gtggtaatcc gggcaaacgg ctgattaacc gttcggtgga
    52801 gcgggcgctg cattttcgca aagaggtcca gcggttgcgg gaagagtctg acggttggtt
    52861 tttcgatatc tggcaaccgc cgcaggtgga tgaagccgaa tgctggcccg ttgcgcctgg
    52921 cgaacagtgg cacggcttta acgatgcgga tgccgatcat atgtttctcg atccggttaa
    52981 agtcactatt ttgacaccgg ggatggacga acagggcaat atgagcgagg aggggatccc
    53041 ggcggcgctg gtggcaaaat tcctcgacga acgtgggatc gtagtagaga aaaccggccc
    53101 ttataacctg ctgtttctct ttagtattgg tatcgataaa accaaagcaa tgggattatt
    53161 gcgtgggttg acggaattta aacgctctta cgatctcaac ctgcggatca aaaatatgct
    53221 acccgatctc tatgctgaag atcccgattt ctaccgcaat atgcgtatcc aggatctggc
    53281 gcaagggatc cataagctga ttcgtaaaca cgatcttccc ggtttgatgt tgcgggcatt
    53341 cgatactttg ccggaaatga tcatgacgcc acatcaggca tggcagagac agattaaagg
    53401 tgaagtagaa accattgcgc tggaacaact ggtcggtaga gtatcggcaa atatgatcct
    53461 gccttatcca ccgggcgtac cgctgttgat gccaggagaa atgctgacca aagagagccg
    53521 cacggtactc gattttctac tgatgctttg ttccgtaggg caacattacc ccggttttga
    53581 aacggatatt cacggcgcga aacaggacga agacggcgtt taccgcgtac gagtcctaaa
    53641 aatggcggga taacttgcca gagcggcttc cgggcgagta acgtgctgtt aacaaataaa
    53701 ggagacgtta tgctgggttt aaaacaggtt caccatattg cgattattgc gacggattat
    53761 gcggtgagca aagctttcta ctgcgatatt cttggtttca cgctgcaaag cgaagtctat
    53821 cgcgaagcgc gcgactcgtg gaaaggggat ttggcgctta atgggcaata tgtgattgag
    53881 cttttctctt ttccgttccc gccggagcgc cccagccgac cggaagcttg cggcctgcgt
    53941 catctggctt ttagcgttga tgacatcgat gcggcagtgg cgcaccttga aagccataac
    54001 gtgaagtgtg aagccatccg tgtcgatcca tacacgcaaa aacgcttcac cttctttaac
    54061 gatccggacg ggctgccgtt ggaactgtat gagcagtaag gcttgtcatc gccgcatttg
    54121 cccggtaacg tgccgggcat tgctactgta aaatcgcacc atcatgacac tcacgctcaa
    54181 tagacaactt ctcacctcac gccagattct ggtggccttt agcggcgggc ttgactccac
    54241 cgttctgctg catcagttgg tgcagtggcg gacggaaaat ccgggcgtca ctctgcgcgc
    54301 tattcatgtg catcacggtt taagtgccaa tgccgatgcc tgggttacgc attgcgaaaa
    54361 cgtctgccaa cagtggcagg tgccgctggt ggtcgaacgc gtacaacttg cgcaagaggg
    54421 gctggggatt gaggcccagg cgcggcaggc gcgttatcag gcatttgccc gtaccctatt
    54481 gcccggcgaa gtgctggtca ccgcgcaaca tctcgacgat caatgtgaaa cctttctgct
    54541 ggcgctaaaa cgcggtagcg gccctgccgg gctttcggct atgggggaag tctcggagtt
    54601 tgccggaaca cggcttatcc gcccgttgct tgcccgcacg cggggggaac tggcgcagtg
    54661 ggcattggcg catggtttac gctggattga agacgaaagt aatcaggacg actcatacga
    54721 tcgtaacttt ctgcgcctgc gtgtagtgcc gttattgcag cagcgttggc cgcattttgc
    54781 cgaagcaacg gcccgcagcg ccgcactttg tgctgaacaa gagagtctgt tggatgaact
    54841 gctggcggat gatttagcac actgccaaac gtcgcagggg acgctgcaga ttgcgccaat
    54901 gctagcgatg agtgatgccc gtcgcgcggc gattatccgc cgctggctgg cagggcagaa
    54961 tgcaccgatg ccttcccgcg acgcgctggt gcggatctgg caggaagtgg cgctggcgcg
    55021 ggaagatgcc tccccctgtt tacgcttggg cgcgtttgaa atccgccgat atcagtcgca
    55081 actgtggtgg attaaatccg tcaccgggca aagtgaaacc attgtgccgt ggcagacgtg
    55141 gctacaaccg ctggaattac cggcggggct gggaagcgta cagcttactg cgggaggcga
    55201 tattcgccct ccgcgtgcag acgaagcggt cagcgtgcgt ttcaaagcgg caggattgct
    55261 gcatattgtc gggcgcaacg gcggacgtaa gctgaagaaa atctggcaag agttaggcgt
    55321 gccgccgtgg ctacgtgaca ccacgccact gctgttttat ggcgaaacgc tgattgcggc
    55381 ggcaggggta tttgtgacgc aagaaggtgt ggctgaaggt gagaatggcg taagttttgt
    55441 ctggcagaaa acgcttagtt aagtgaaagc cggataagac gcatcaaacg tcgcatccgg
    55501 cgaaagtcaa tcaggactcg ctcaccacca ccgtaccgat ttccgggtgg ctaaagctgg
    55561 taattttatc cagacgtagc tcacgggttt cgccagcggc ctcgacgacc agatactcca
    55621 catttttgcg ggagactaaa tcgctggctt ttgcctgcaa tttttcgcca tctttcagct
    55681 caagtgtcag cattaaatga tgctggcagg cgagctcaag attatcgtaa tcatcacaat
    55741 tgattggttg atacgtatca ttcattgaca taatcgctca ccagtaagtt tgccgcagcg
    55801 tatgctgctt tttccctgac agcctcagaa agggcgtcgt cggcagccat ttcattcagc
    55861 actttcaaaa cgcagcccag cgcgtccgga acgtatccta agtctccgct ggcgatttcc
    55921 gcgtaccgct tgcgtattaa ctcacaatat ttttccacat gccctcctgt cagcactctg
    55981 acttaaccgt ggatgcaagt ctaagcctac gaagataaac tctgtttcgc aaggtgacta
    56041 taccacactc atttctgcaa tatcagcgtc gcaactgcac gtattccgtt acaatggcct
    56101 cctgattcga aaggagtttt cttatggcgc ttaaagcgac aatttataaa gcgacggtta
    56161 atgtggccga tctcgaccgc aaccagtttc tcgatgcctc tctgacgctg gcgcgccacc
    56221 cttcagaaac ccaggagcgt atgatgctgc gcttgctggc gtggctgaaa tatgctgatg
    56281 aacgtctgca atttacccgt ggtttgtgtg ccgatgatga gccggaagcg tggctgcgta
    56341 acgatcacat gggcattgat ttgtggattg agctggggct gccggatgag cggcggatta
    56401 agaaagcctg cacccaggcc gcagaagtgg cgctgtttac ctataatagt cgggcggcgc
    56461 aaatctggtg gcagcaaaat cagagcaaat gtgtgcagtt tgccaatctt tccgtctggt
    56521 atctggacga tgaacaactg gcgaaagtaa gcgcctttgc cgatcgtacc atgacgctgc
    56581 aggcaacgat tcaggatggc gtgatctggt tatcggacga taagaataat ctggaagtta
    56641 acttaaccgc ctggcaacaa ccttcatgat tgtgatttcc cgacatgttg ctattcccga
    56701 tggtgagctt gagatcaccg ccattcgtgc gcagggcgcg ggcgggcagc atgttaataa
    56761 gacctcaacg gccattcatc tgcgttttga cattcgggcg tccagcctgc cagagtatta
    56821 caaagagcgt ctactcgccg acagccatca tttgatcagc agtgatggcg tgattgtcat
    56881 taaggcacag gaataccgca gtcaggaact gaaccgcgaa gccgcgctag cccggctggt
    56941 ggcagtgatt aaagatttaa caacagaaca aaaagcccga cgacccacgc ggcccacccg
    57001 tgcatcgaaa gagcgcaggc tggcatcgaa agcacaaaaa tcaagcgtga aggcgatgcg
    57061 cggcaaagta cgcagcggtc gggaataaaa agaaggaata gatggtgaaa aaagcgatag
    57121 tgacagcgat ggctgtaatc agcctcttta ctctgatggg atgtaataac cgggccgaag
    57181 tcgatacgct ttctccagca caggctgccg aactgaaacc gatgccgcaa agttggcgcg
    57241 gcgtgctgcc gtgtgctgat tgcgaaggaa tcgaaacctc tctgttcctc gaaaaagacg
    57301 gaacatgggt gatgaatgag cgttatctcg gtgctcgtga agaaccttcc tccttcgctt
    57361 cctacggtac atgggcgcga accgctgaca agctggtatt aaccgacagc aaaggtgaaa
    57421 agtcatatta tcgggcgaaa ggcgatgcgc tggagatgct cgatcgtgaa ggcaatccga
    57481 ttgaatcgca gttcaactat acgctggaac cggcacaatc cagtttaccc ctgacgccga
    57541 tgaccctgcg gggcatgtat ttttatatgg ctgatgcggc gaccttcact gattgcgcga
    57601 ccggaaaacg tttcatggta gcgaataacg cagagctgga gcgtagctac ctggctgcgc
    57661 gcggtcacag tgaaaaaccg gtgttactgt cagtagaagg tcactttacg cttgaggcta
    57721 atccggatac tggtgcgcca accaaagtat tagcgcccga tacggcgggt aaattttacc
    57781 cgaataagga ttgcagtagt ttggggcagt aaacctgctt aaggcagaaa caaacgcaaa
    57841 attgcctgat gcgct
//
LOCUS       DAZGSP010000019        57750 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZGSP010000019 DAZGSP010000000
VERSION     DAZGSP010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.019"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..266
                     /gene="ydjO"
                     /locus_tag="ACHK74_001296"
     CDS             <1..266
                     /gene="ydjO"
                     /locus_tag="ACHK74_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFP0220154.1"
                     /translation="NPLNLPGMGSGVLEDIMSIPDSSLRKRLGYEVLSFSLQAHSLSQ
                     ECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(270..1661)
                     /gene="tcyP"
                     /locus_tag="ACHK74_001297"
     CDS             complement(270..1661)
                     /gene="tcyP"
                     /locus_tag="ACHK74_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFP0220155.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1794..2384)
                     /gene="ydjM"
                     /locus_tag="ACHK74_001298"
     CDS             complement(1794..2384)
                     /gene="ydjM"
                     /locus_tag="ACHK74_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0220156.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2547..3215)
                     /gene="hxpB"
                     /locus_tag="ACHK74_001299"
     CDS             complement(2547..3215)
                     /gene="hxpB"
                     /locus_tag="ACHK74_001299"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFP0220157.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3362..3898
                     /gene="yniB"
                     /locus_tag="ACHK74_001300"
     CDS             3362..3898
                     /gene="yniB"
                     /locus_tag="ACHK74_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFP0220158.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(3939..4799)
                     /gene="yniA"
                     /locus_tag="ACHK74_001301"
     CDS             complement(3939..4799)
                     /gene="yniA"
                     /locus_tag="ACHK74_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFP0220159.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4905..5195)
                     /gene="ghoS"
                     /locus_tag="ACHK74_001302"
     CDS             complement(4905..5195)
                     /gene="ghoS"
                     /locus_tag="ACHK74_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP0220160.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5296..6225)
                     /gene="pfkB"
                     /locus_tag="ACHK74_001303"
     CDS             complement(5296..6225)
                     /gene="pfkB"
                     /locus_tag="ACHK74_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFP0220161.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            6512..7270
                     /gene="ydiY"
                     /locus_tag="ACHK74_001304"
     CDS             6512..7270
                     /gene="ydiY"
                     /locus_tag="ACHK74_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFP0220162.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7323..7430)
                     /gene="yniD"
                     /locus_tag="ACHK74_001305"
     CDS             complement(7323..7430)
                     /gene="yniD"
                     /locus_tag="ACHK74_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFP0220163.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(7606..9504)
                     /gene="espL1"
                     /locus_tag="ACHK74_001306"
                     /pseudo
     CDS             complement(7606..9504)
                     /gene="espL1"
                     /locus_tag="ACHK74_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            10027..11955
                     /gene="thrS"
                     /locus_tag="ACHK74_001307"
     CDS             10027..11955
                     /gene="thrS"
                     /locus_tag="ACHK74_001307"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFP0220164.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            11959..12501
                     /gene="infC"
                     /locus_tag="ACHK74_001308"
     CDS             11959..12501
                     /gene="infC"
                     /locus_tag="ACHK74_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFP0220165.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            12598..12795
                     /gene="rpmI"
                     /locus_tag="ACHK74_001309"
     CDS             12598..12795
                     /gene="rpmI"
                     /locus_tag="ACHK74_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFP0220166.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            12848..13204
                     /gene="rplT"
                     /locus_tag="ACHK74_001310"
     CDS             12848..13204
                     /gene="rplT"
                     /locus_tag="ACHK74_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFP0220167.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    13321..13445
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            13327..13371
                     /gene="pheM"
                     /locus_tag="ACHK74_001311"
     CDS             13327..13371
                     /gene="pheM"
                     /locus_tag="ACHK74_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFP0220168.1"
                     /translation="MNAAIFRFFFYFST"
     gene            13655..14638
                     /gene="pheS"
                     /locus_tag="ACHK74_001312"
     CDS             13655..14638
                     /gene="pheS"
                     /locus_tag="ACHK74_001312"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFP0220169.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            14653..17040
                     /gene="pheT"
                     /locus_tag="ACHK74_001313"
     CDS             14653..17040
                     /gene="pheT"
                     /locus_tag="ACHK74_001313"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFP0220170.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            17045..17344
                     /gene="ihfA"
                     /locus_tag="ACHK74_001314"
     CDS             17045..17344
                     /gene="ihfA"
                     /locus_tag="ACHK74_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFP0220171.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            17445..18425
                     /gene="btuC"
                     /locus_tag="ACHK74_001315"
     CDS             17445..18425
                     /gene="btuC"
                     /locus_tag="ACHK74_001315"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFP0220172.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            18488..19039
                     /gene="btuE"
                     /locus_tag="ACHK74_001316"
     CDS             18488..19039
                     /gene="btuE"
                     /locus_tag="ACHK74_001316"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFP0220173.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            19039..19788
                     /gene="btuD"
                     /locus_tag="ACHK74_001317"
     CDS             19039..19788
                     /gene="btuD"
                     /locus_tag="ACHK74_001317"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFP0220174.1"
                     /translation="MSIVMQLQDVAETTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            19866..20330
                     /gene="nlpC"
                     /locus_tag="ACHK74_001318"
     CDS             19866..20330
                     /gene="nlpC"
                     /locus_tag="ACHK74_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP0220175.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            20577..21290
                     /gene="ydiV"
                     /locus_tag="ACHK74_001319"
     CDS             20577..21290
                     /gene="ydiV"
                     /locus_tag="ACHK74_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFP0220176.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQSQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            21353..22789
                     /gene="selO"
                     /locus_tag="ACHK74_001320"
     CDS             21353..22789
                     /gene="selO"
                     /locus_tag="ACHK74_001320"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFP0220177.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLIRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRRRLQQDEVSDIERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(22793..22984)
                     /gene="hemP"
                     /locus_tag="ACHK74_001321"
     CDS             complement(22793..22984)
                     /gene="hemP"
                     /locus_tag="ACHK74_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFP0220178.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(23116..24162)
                     /gene="aroH"
                     /locus_tag="ACHK74_001322"
     CDS             complement(23116..24162)
                     /gene="aroH"
                     /locus_tag="ACHK74_001322"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFP0220179.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(24319..25152)
                     /gene="ppsR"
                     /locus_tag="ACHK74_001323"
     CDS             complement(24319..25152)
                     /gene="ppsR"
                     /locus_tag="ACHK74_001323"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFP0220180.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            25485..27863
                     /gene="ppsA"
                     /locus_tag="ACHK74_001324"
     CDS             25485..27863
                     /gene="ppsA"
                     /locus_tag="ACHK74_001324"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFP0220181.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(27920..29566)
                     /gene="fadK"
                     /locus_tag="ACHK74_001325"
     CDS             complement(27920..29566)
                     /gene="fadK"
                     /locus_tag="ACHK74_001325"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFP0220182.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(29623..29916)
                     /gene="ydiT"
                     /locus_tag="ACHK74_001326"
     CDS             complement(29623..29916)
                     /gene="ydiT"
                     /locus_tag="ACHK74_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP0220183.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(29913..31202)
                     /gene="ydiS"
                     /locus_tag="ACHK74_001327"
     CDS             complement(29913..31202)
                     /gene="ydiS"
                     /locus_tag="ACHK74_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP0220184.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREKPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALIENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(31258..32196)
                     /gene="ydiR"
                     /locus_tag="ACHK74_001328"
     CDS             complement(31258..32196)
                     /gene="ydiR"
                     /locus_tag="ACHK74_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFP0220185.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(32216..32980)
                     /gene="ydiQ"
                     /locus_tag="ACHK74_001329"
     CDS             complement(32216..32980)
                     /gene="ydiQ"
                     /locus_tag="ACHK74_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFP0220186.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            33296..34207
                     /gene="ydiP"
                     /locus_tag="ACHK74_001330"
                     /pseudo
     CDS             33296..34207
                     /gene="ydiP"
                     /locus_tag="ACHK74_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(34250..35401)
                     /gene="ydiO"
                     /locus_tag="ACHK74_001331"
     CDS             complement(34250..35401)
                     /gene="ydiO"
                     /locus_tag="ACHK74_001331"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFP0220187.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECTFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(35415..37010)
                     /gene="ydiF"
                     /locus_tag="ACHK74_001332"
     CDS             complement(35415..37010)
                     /gene="ydiF"
                     /locus_tag="ACHK74_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFP0220188.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMNERLFIDAAMGFVLPEAAH"
     gene            complement(37154..37912)
                     /gene="aroD"
                     /locus_tag="ACHK74_001333"
     CDS             complement(37154..37912)
                     /gene="aroD"
                     /locus_tag="ACHK74_001333"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFP0220189.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(37943..38809)
                     /gene="ydiB"
                     /locus_tag="ACHK74_001334"
     CDS             complement(37943..38809)
                     /gene="ydiB"
                     /locus_tag="ACHK74_001334"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFP0220190.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(38821..40086)
                     /gene="ydiN"
                     /locus_tag="ACHK74_001335"
     CDS             complement(38821..40086)
                     /gene="ydiN"
                     /locus_tag="ACHK74_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0220191.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     IRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(40313..41527)
                     /gene="ydiM"
                     /locus_tag="ACHK74_001336"
     CDS             complement(40313..41527)
                     /gene="ydiM"
                     /locus_tag="ACHK74_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0220192.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMLINALFLYRCTFPPHPGRRLPVIKKTTRSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLSQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(41627..41983)
                     /gene="ydiL"
                     /locus_tag="ACHK74_001337"
     CDS             complement(41627..41983)
                     /gene="ydiL"
                     /locus_tag="ACHK74_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFP0220193.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(42119..42226)
                     /gene="rprA"
                     /locus_tag="ACHK74_001338"
     ncRNA           complement(42119..42226)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHK74_001338"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(42412..43524)
                     /gene="ydiK"
                     /locus_tag="ACHK74_001339"
     CDS             complement(42412..43524)
                     /gene="ydiK"
                     /locus_tag="ACHK74_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFP0220194.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            43913..46969
                     /gene="ydiJ"
                     /locus_tag="ACHK74_001340"
     CDS             43913..46969
                     /gene="ydiJ"
                     /locus_tag="ACHK74_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFP0220195.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            46966..47376
                     /gene="menI"
                     /locus_tag="ACHK74_001341"
     CDS             46966..47376
                     /gene="menI"
                     /locus_tag="ACHK74_001341"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFP0220196.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            47476..47664
                     /gene="ydiH"
                     /locus_tag="ACHK74_001342"
     CDS             47476..47664
                     /gene="ydiH"
                     /locus_tag="ACHK74_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFP0220197.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            48212..48580
                     /gene="sufA"
                     /locus_tag="ACHK74_001343"
     CDS             48212..48580
                     /gene="sufA"
                     /locus_tag="ACHK74_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFP0220198.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            48589..50076
                     /gene="sufB"
                     /locus_tag="ACHK74_001344"
     CDS             48589..50076
                     /gene="sufB"
                     /locus_tag="ACHK74_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFP0220199.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            50086..50832
                     /gene="sufC"
                     /locus_tag="ACHK74_001345"
     CDS             50086..50832
                     /gene="sufC"
                     /locus_tag="ACHK74_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFP0220200.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            50807..52078
                     /gene="sufD"
                     /locus_tag="ACHK74_001346"
     CDS             50807..52078
                     /gene="sufD"
                     /locus_tag="ACHK74_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFP0220201.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            52075..53295
                     /gene="sufS"
                     /locus_tag="ACHK74_001347"
     CDS             52075..53295
                     /gene="sufS"
                     /locus_tag="ACHK74_001347"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFP0220202.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            53308..53724
                     /gene="sufE"
                     /locus_tag="ACHK74_001348"
     CDS             53308..53724
                     /gene="sufE"
                     /locus_tag="ACHK74_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFP0220203.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            53873..54877
                     /gene="ldtE"
                     /locus_tag="ACHK74_001349"
     CDS             53873..54877
                     /gene="ldtE"
                     /locus_tag="ACHK74_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFP0220204.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(54941..55177)
                     /gene="lpp"
                     /locus_tag="ACHK74_001350"
     CDS             complement(54941..55177)
                     /gene="lpp"
                     /locus_tag="ACHK74_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFP0220205.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(55488..56900)
                     /gene="pykF"
                     /locus_tag="ACHK74_001351"
     CDS             complement(55488..56900)
                     /gene="pykF"
                     /locus_tag="ACHK74_001351"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFP0220206.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKIVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(56908..57102)
                     /locus_tag="ACHK74_001352"
     CDS             complement(56908..57102)
                     /locus_tag="ACHK74_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220207.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            57457..57666
                     /gene="fumD"
                     /locus_tag="ACHK74_001353"
     CDS             57457..57666
                     /gene="fumD"
                     /locus_tag="ACHK74_001353"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFP0220208.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT        14760 a        13840 c        14671 g        14479 t
ORIGIN      
        1 ttaatccact aaatcttccg ggaatgggaa gtggtgtgct tgaagatatt atgagtattc
       61 cagatagttc acttagaaaa cgtctcgggt atgaggtttt aagcttctca ctacaggcac
      121 attcattgag ccaggaatgt attgataagt tggacatttt tttcgctgac gatctgttta
      181 aatatgagtc agtatgcatc gccgcgatgg aacatttaaa aagtaaagca acggccccta
      241 tacaaaacgg accgttgcca gcataagaat taacggtgtg ccagttcggc gtcgtcttcg
      301 ctatccagaa tggctttatc ggtttgcttc agccactggc tggtcagcgt gccagctgtc
      361 atcgagccac taacgtttag cgccgtacgg cccatgtcga taagcggttc aacggagatt
      421 aacagcgcca ccagggttac tggcaggccc atcgcaggca gcacaatcag tgcggcgaaa
      481 gttgcaccac caccgacacc ggcaacgcct gcggaactaa cggtaacaat accgaccagc
      541 gtcgcaatcc acatcgggtc cagcgggtta atgccaaccg taggcgcaac catcaccgcc
      601 agcattgccg gatacaaacc ggcgcagccg ttctgaccaa tggttgcacc gaaagaggcg
      661 gcgaaactgg cgatggattc aggaacgccc agacgacgcg tttgtgcttc cacattcagt
      721 gggatagacg cagcactgga acggctggta aaggcaaacg tcagcacagg ccatacctta
      781 cggaagtact tcagcggact cacgccatta atgcccagca gaatgccatg cactgcaaac
      841 ataatcagca gaccgaggta ggacgcgaca acgaaacttc ccagtttgat gatgtcttgc
      901 aagttagaac ctgcaaccac tttggtcatt agagccagaa cgccgtaagg ggtcaattgc
      961 atgacaaggc gaaccagttt catcacccag ctttgtaggg tatcgatagc ggctaagacg
     1021 cgttcacctt tcggcgcatc atccttcagc agtttcaatg cagctacgcc gaggaatgcg
     1081 gcaaaaatta ccacgctgat aattgacgtc ggattggctc cggtaagatc ggcaaacggg
     1141 tttttcggga taaaggacaa gaccagctgc ggaacgctca gatcagagac tttaccaaca
     1201 tagttacttt caatggcgtt cagacgtgca gtttctgcac caccctgaac cagaccttca
     1261 gccgtcaaac caaacaggtt ggtgaccagc acaccgacca gcgccgcaat cagcgtggta
     1321 aacaaaagcg taccgatggt cagaaaactg attttgccta actgagatgc gttatgcaga
     1381 cgggcaaccg cgctcagaat agaggcgaag actaacggca taacgatcat ttgcagcagt
     1441 tgaacatagc cgttaccaac gatgttaaac cactgtacag aatctttaag tacctggctg
     1501 tcagaaccat aaatggtatg cagggcaagg ccaaaaacca cacccatcac cagacccacc
     1561 agcacttttt tcgccagact ccactgttta tggcgggtct gagccagcgc aaacagcagt
     1621 acaacgaaca ccacgatgtt cgcaattaat ggaaagttca tccccgttct cctgatctta
     1681 ttatttctat cactgcgtag cagtcatagt gttggcgcaa gattaacaga actgtgagct
     1741 ggctcttata ttcaaatgga attgcttatt ccagaaaggt gatttttgtc tttttagtat
     1801 tcaacctgat gcttaataag ccgatgaaac tggatctgca aggtattgat catttgcgat
     1861 gaccaacgaa cagcgctgtt ctcgggtaat gaatggggca tccataccga ccagacaaat
     1921 aatgccatgc agataaaacg ttccagttgg ttgccctttt ggggaaccag gataggcaag
     1981 cggaaacgcc agcggcatgg ccagagcagg ggaacaccgg cgggtgtcag catatcggca
     2041 agtatgtggc tcaaataacc cagcaccatt ccttgtagcg catcagccgg aatgaaccag
     2101 ccttccggaa ccttaaggta aaaggttgcc agcagcgcaa ataccgccag cagactgtgg
     2161 gtaaaaccac gatgcccaaa agcgcgggcg atcggttttg atatccattt taatcgctgc
     2221 ccaagaaacg actttgggtg atcgatgtcc ggtaacaaac acgtcaggat tgcggaaggg
     2281 acaatatgcc accagtcacc ctgtgccagc acgggcgtca gctcggcatt tttggcaaat
     2341 accgcacaag caatagaaaa gagaaggtga ccttccgccg tcatgataaa actcactaaa
     2401 ctgtcgatac gtacagtata ggatttttat acagtgcacg gaagtggtga cggtgtaact
     2461 ctttctctta aattgacgat taatccgtca gttgaatgca aattcagata aagaaccagg
     2521 ggcagcgaga tcactgcccc tggtcattaa ccgagaaggt cttttgcggt gagttctgtc
     2581 agcgatgaga gtttgacgtt tgctaataca aaacgtggat cattttgcgc ttctggcgca
     2641 ggaacgacaa tggaacgcat gcgggctgct ttagaggcga tcatgccatt tactgaatct
     2701 tccagcgcta cgcaggtcag tgggtcaacg cccagttttg ctgcgcagtc gagatatact
     2761 tgcggatgcg gcttgctgta aggcagtttt tcagccgagg cgagggcatc gaaactgtcg
     2821 cgtaagtcaa acatggtcaa cactttttcc agcatatgta gtggtgacgc ggaggccagt
     2881 cccaccaata aaccttgttc tttgcataac gcaacggctt cgcgcacgcc tggtaataat
     2941 ggacgtgtct cttcaaccag tgaaatggca cgggcaataa cccgttctac tacttcctga
     3001 cggcttggcc cattccacgg ttgccgggcg taccaaagat cgaccaccat atcgatgcgt
     3061 aaacctaagg tgtccggcag ctcgttacga cgggagatat ccacccctag gcttgccatc
     3121 acatccagtt cggctcgatc ccataaaggt tctgagtcga taagtaatcc atccatatca
     3181 aaaattgcag caagaatctg acgcggggtt gacattacaa cctctccttt agagcattat
     3241 gtgtaaggta ttaagccgtg cttatgatta agcgcctatc atatcgcctt taaagaatgg
     3301 gcgaaaacag atatctacag gtaaactaag tgaaacgacg cgtcttaatc aaggagaact
     3361 catgacgtat caacaagctg gacgcattgc tgttttgaaa aggattttgg gttgggtgat
     3421 ttttatacct gcattgatct ctacattgat ttctttgctg aaatttatga atacccggca
     3481 ggaaaaccag gaaggcatta atgcggtcat gctcgacttt actcatgtca tgatcgatat
     3541 gatgcaggcg aatacgcctt ttttgaatct gttctggtat aactccccga cgcccaattt
     3601 taatggtggc gtgaacgtta tgttctgggt gattttcatc ctgatttttg tcggactggc
     3661 gttgcaggat tccggtgccc ggatgagtcg ccaggcgcgc ttcttgcggg aaggtgttga
     3721 agatcaactc attctggaaa aagccaaagg ggaagagggg ctaacgcgtg aacaaattga
     3781 gtcccgtatt gttgttccgc accatactat tttcctgcag tttttctcac tatatatcct
     3841 gccggttatt tgtattgctg caggttatgt gttcttttct ctgcttgggt ttatttaatc
     3901 aaaaggccgc acggagcggc cattatcctc aacccatatc atgctgctaa taatctatcc
     3961 aatgactgct gagcaataac caaatgctga ccgccaaata atcttgcacg atttaacagc
     4021 gtgtagagtt ggtaaaccgg ttgacgttca aggaaatcgg caggtagcgg ggatactgac
     4081 tgatagccgt catagatttg tggcggctgt tcagtatgca gcggtaacat cgccaggtcg
     4141 cactctcggt caccccagta gcaggcaggg tcgaaaatgt acgggccatc cggacccagc
     4201 gcacagttgc cggaccataa atcgccgtgc aacagagaag gctgcggttg atgcgaggcc
     4261 agacgctgct gaatatgctc gacgatggcg tcgatattgc cgaaagcgat ccctttctct
     4321 gctgccagtt ccaactgcca gccaatccgt tgttcagcaa aaaacgttga ccagcgacgt
     4381 tgccaggtgt tgggctgtgg agttgtggag agcgagttat caaaatcgag gccaaattgt
     4441 ggttggtcac tccattgatg taaacgcgca atttgctgac caagaataaa tgcgctatgc
     4501 gcatccagcg gacgaggtgg gagataatcc atcaccagaa aactgtagtc acggtcagcg
     4561 cctactgccc aaaccttagg cacggtgacg gttttactac gcgacagtaa ctccagttgg
     4621 tcggcttcgg cggtaaaacc gggaagcagt tccctttcat cacatttgac gaaaaagtca
     4681 tgtcctgcat agcgcaaatg ccatgcggca tggacttctc cgccaggcag ttcattacgc
     4741 agttcgattt cgccttcacc taactgctcg ctcaaaagac gactgattgc ctgccacatg
     4801 atgtctctcc catgttgtct gccagatcat aaggttagcg caagaatgcg gtgtaaaaac
     4861 acgagctaac gcacaccaca gactgattat tgcccaaaaa ccatttattt tctgttgcta
     4921 ttccattcct cccaggtcgt gatggtgatt tcaggatctt tgccggttgc actttgggta
     4981 agccccgaaa ccagttctct aatttcttct tcactttggg tactgataag tccaaaagtg
     5041 ttagtcccca gctcatggat atttccctca tcatccgtca tggtgagcaa aaaaccgtcg
     5101 cgagtcaggt aattattcaa ctcgttaatt tcagtcaatg tatcctcatg caacatgacg
     5161 gttatgacat aacggacaag atcgccactg gccatagttc acccctttgt tatcacatga
     5221 cttttgagca tagtcggaga aacgcgttgc cgacaggttg gtggtgattc ccccaatgct
     5281 gggggaatgt ttttgttagc gggaaaggta agcgtaaatt ttttgcgtat cgtcatggga
     5341 gcacagacgt gttccctgat tgagtgtggc tgcactcccc gcagctacgc caaaacggac
     5401 catctcttca agagaggcat tttctgccag tttcagtgtc atcgcgccga ccatgctgtc
     5461 accagcgcca acggtactct ggcttttcac cggtggtggc accacctgaa tgcagttttc
     5521 actatcaaca cccagcgctc cttgtggacc aagggaaacg acaacccgtt tggccttgcc
     5581 gctattaacg atttcctgcg cggctttgcg gacatcgtct ggctgggtga gttcgcgatt
     5641 caccagcgca ctgagttctt tttggttagg cttaaccaac tcgatgttac caatcgccag
     5701 tgctgcactt aacgcttcgc cagaactgtc gatgatgcag cggatccctt ggttttgcgc
     5761 agcggaaatc agttgggtta atttttccag cttcacacct ggcggcaggc ttccgcttat
     5821 gaccaggatg gccccggatt caatttccag aacttgctct tcaagctggc gaaactcatc
     5881 ttcatttaat gccgcgcctg gcataacaaa acgatactgc tcaccgcttg cttccacatg
     5941 tacgtgtaaa ttctgccggg tccagtcttt ggcttctaca gtagcgacgg ggacattttc
     6001 atccgccaac agagaaacca ggtgttcgcc ggtcgcgcca cccgccggga agatcgctgt
     6061 ggcactgcct ccaagatggg caatggcgcg ggcgacgttg atgccgccgc ccccgggttc
     6121 gaacaccggt gcggtacagc gcagttttcc ttcgggataa atttgcgggg taattgttgc
     6181 gctatcgaga gagggcgcaa gtgtcaacgt atagatacgt accatcattt cctcctatag
     6241 gctgatttca gtctggcacc gaatcagccg aaagtgaaga aattaacaat atgatttatt
     6301 agagctttat ttaaaatttt gcagataaat atatataaat aaaaatctgt tttcgccaca
     6361 tgcttattca aaaaatgaaa taagaataca ttgctaagtt attcgcgcct ttttataatt
     6421 cgttaccttt ccagggacgc taatctttga tccttaagaa agtttccggg accgcgatgg
     6481 tctctttttt tgttgtacgg actcttgata aatgaagctt ttgaagacag ttcctgccat
     6541 cgttatgttg gcggggggca tgtttgcgtc actgaatgca gctgccgatg attccgtttt
     6601 tactgtcatg gacgatcctg ccagcgctaa aaaacctttc gaaggtaatc tgaacgcagg
     6661 atatcttgca caatcaggca atacgaaaag ctcctcactt acggctgaca ccaccatgac
     6721 ctggtatgga caaaccactg cctggtcgct gtgggggaac gccagtaata cttcttctaa
     6781 tgatgagcgt tcttcagaaa aatatgcggc gggcgggcgt agccgtttta atttaactga
     6841 ttatgactat ttatttggtc aggcaagctg gctgacagac cgttataacg gctatcgaga
     6901 gcgcgatgtg ttgaccgcgg gttatggtcg tcagtttttg aatggcccgg tacacagttt
     6961 ccgctttgaa ttcggtccag gtgtgcgtta cgacaaatat accgacaatg ccagtgaaac
     7021 tcagccgctg ggctatgcct ccggtgctta tgcatggcag ttgactgaca acgctaaatt
     7081 tacgcagggt gtttccgtct ttggcgcgga agacacaacg ctgaactccg aaagcgcatt
     7141 aaatgttgct attaatgaac attttggact gaaagtggct tacaacgtca catggaactc
     7201 tgaacctcca gagtctgcac cggaacatac cgatcgtcgg acaaccttat cgctgggcta
     7261 cagcatgtaa aattatctgc cggaccgtgt tatgcggtct ggctgtcttc gtttgtcact
     7321 gctcagcccc aaatcattgc tgcccaacgt agcaacaaca tagcgccaca gattgccagt
     7381 agcaccacca ccatcttcca gtgtttttta tcaaagcgtt tagtcggcat accagtgtcc
     7441 tgcatatctc ttgtgcgtga taacggagtg taacaaaagg gacaggcatc aagggatttt
     7501 ttgttattac agccgtcggg tggcgttgta cacctgatca ctgtttctgc gttttatgaa
     7561 aaaaataaaa ttatgtatcg atatacaaat atcataaata gctggttata aatacgtttt
     7621 tagttcatca gcactaaaac tcaggctttg gctgattgca ttaatagcag cataatatgt
     7681 ttctacagtt ttataatgat tatgatgaat ggctatatga acagctgaca tgttttcatg
     7741 atttttagcg gccaacagtg tgaataattg acgttgacta aaagaatatt tttttgcaaa
     7801 agtactcagt gttgatatat aagataacac gacgtcttta ttacccttgc tcatggcgat
     7861 gtataaagca ggggttccat gtgctgtcgc gccttttaat aagtccatga tgttgatttt
     7921 gctgagttta tatttaacgg cgatcccata aactttagag aggaaccgtg tgacacacaa
     7981 agggtgatta ttttccattg cagcaaataa ccctggttca ccatcacgtg ttttcatggc
     8041 taaaatatcc atcaccattt ttttgtttaa aagttcttgt gctattggag tggttaatgc
     8101 attcagaaag atatcgatta tttcatcgaa tccatacaac atggagatat agagtccagg
     8161 tgtttcgtta gcacttttag tttgcagtaa tttaaccaga ttatcttcat gaatgatatg
     8221 attgtatacc agtgactgga tttcttgcat aaatattttt acgttgtcag tttgtccgtt
     8281 catcatagcc agaaataaag caggtgtacc aacgctattc tgcgcagtta ttatttcaat
     8341 gatttctttt tcagggagta tgggcaactg tttgaatagt gtaataagct attgagtctg
     8401 gcctgtggcc attattaaca acattgccga tggatgtata ggggcaaatg gcattcgtgt
     8461 cgtgataccc gctaaaatag caactggaac tccctcaggt aatagaggaa gaatagcaat
     8521 tgcacagttg cgaacatacc tatcagccca ggtctgagca tcgggagctg aactgatgaa
     8581 cttatcaagc gagagaaagc gccgtgctgt cctgtgggtg cctctgacgg caacgttggt
     8641 atcgttcggg tcgtaaacag acacaacata atttaattgt ccatacactg actgctttat
     8701 tcgtagcctt actgacaaag tatgattgtc tatatataaa atcgcggaca tatgactgac
     8761 attgtttgcc atcattcgac aaaagaaaat atctaaagcg aagccaaaat ccttactcgc
     8821 gataacgcga ctccctgcag gaggatggcg aagtaatgct ctgaactccg catcccggac
     8881 taaggggata tgctgctgta tggattcaac ggaggagtaa tgtttaatca ggttgacttt
     8941 gccttttcca tttctcaatg aatcaagggc gtattgcaat gacagatggc gacaaaaaat
     9001 agcgtcagaa ggagattgca aaaaacatgc actaccattg agatttactg ttggttgtat
     9061 agttttttct ctatggattt ttttagacag tttcatacaa tggttacgat cttcggcagt
     9121 taactgttcc tgctcaatat aatccagtat gcttcgcaaa tggttagcct taataggcgt
     9181 ggtttccaga aataagggaa tgatttcttt tttatagtgt aatgtaataa gtatattttc
     9241 tgcatggatg ggtgaagtgg caaagttaat gataaaaagg gcagtggagt aacaattttg
     9301 tgatattttt cgacagtttg cggtaaattc ctgagtaata cagtatgaca gcatcgaact
     9361 taagtcagag gtatcaagta gggtgatgag ttggcgcgca aattgattaa taggctctga
     9421 tgaaggcgtt ggtatgtcgc tatcgatgtg caatctatga ggagacttat aatgagttct
     9481 gataatgata tcgttttgac tcataatcta ttcaccttat atatgtctgg atgttttgag
     9541 gatgatgatt aattattatc gccagaacga aatgtgcacg aaaaattgct ataagaattg
     9601 tgtcactttt atcattgtga tatgaccact tcgtcattaa tctcatgatc gtttaaatac
     9661 taccgccaaa tcacgcaaca aactttgctg gattcagttt atgctgctgt aaatccgctc
     9721 gcagtaaacc tttcagacgc tccgtgatgt tatcagttgt tctttaagcg ttttgctggt
     9781 gtactcacta caaacgaatt gcgaatcgaa tcaatgtgaa acggaaaggt acaatctccc
     9841 tttttttagt gttaacatcg ctcaaccggg tggaaggtga gaggttaatg tctttcattt
     9901 gtgtacctga ttgcgaacca atttagcatt tgttggctag atggtttcgc aatgaactgt
     9961 taataaacaa atttttcttt gtatgtgatc tttcgtgtgg gtcaccactg caaataagga
    10021 tataaaatgc ctgttataac tcttcctgat ggcagccaac gccattacga ccacgctgta
    10081 agccccatgg atgttgcgct ggacattggt ccaggtctgg cgaaagcctg tatcgcaggg
    10141 cgcgttaatg gcgaactggt tgatgcttgc gatctgattg aaaacgacgc acaactgtcg
    10201 atcattaccg ccaaagacga agaaggtctg gagatcattc gtcactcctg tgcgcacctg
    10261 ttagggcacg cgattaaaca actttggccg cataccaaaa tggcaatcgg cccggttatt
    10321 gacaacggtt tttattacga cgttgatctt gaccgcacgt taacccagga agatgtcgaa
    10381 gcactcgaga agcggatgca tgagcttgct gagaaaaact acgacgtcat taagaagaaa
    10441 gtcagctggc acgaagcgcg tgaaactttc gccaaccgtg gggagagcta caaagtctcc
    10501 attcttgacg aaaacatcgc ccatgatgac aagccaggtc tgtacttcca tgaagaatat
    10561 gtcgatatgt gccgcggtcc gcacgtaccg aacatgcgtt tctgccatca tttcaaacta
    10621 atgaaaacgg caggggctta ctggcgtggc gacagcaaca acaaaatgtt gcaacgtatt
    10681 tacggtacgg cgtgggcaga caaaaaagcg cttaacgctt acctgcagcg cctggaagaa
    10741 gccgcgaaac gcgaccaccg taaaatcggt aaacagctcg acctgtacca tatgcaggaa
    10801 gaagcgccgg gtatggtatt ctggcacaac gacggctgga ccatcttccg tgaactagaa
    10861 gtgtttgttc gttctaaact gaaagagtac cagtatcagg aagttaaagg tccgttcatg
    10921 atggaccgtg tcctgtggga aaaaaccggt cactgggaca actacaaaga tgcaatgttc
    10981 accacgtctt ctgagaaccg tgaatactgc attaagccga tgaactgccc gggtcacgta
    11041 caaattttca accaggggct gaagtcttat cgcgatctgc cgctgcgtat ggccgagttt
    11101 ggtagctgcc accgtaatga gccgtcaggt tcgctgcatg gcctgatgcg cgtgcgtgga
    11161 tttacccagg atgacgcgca tatcttctgt actgaagaac aaattcgcga tgaagttaac
    11221 ggatgtatcc gtttagtcta tgatatgtac agcacttttg gcttcgagaa gatcgtcgtc
    11281 aaactctcca ctcgtcctga aaaacgtatt ggcagtgatg aaatgtggga tcgtgctgag
    11341 gcggacctgg cggttgcgct ggaagaaaac aacatcccgt ttgaatatca actgggtgaa
    11401 ggcgctttct acggtccgaa aattgaattt accctgtatg actgcctcga tcgtgcatgg
    11461 cagtgcggta cagtacagct ggacttctct ttgccttctc gtctgagcgc ttcttatgta
    11521 ggcgaagaca atgaacgtaa agtaccggta atgattcacc gcgcaattct gggatcgatg
    11581 gaacgtttca tcggtatcct gaccgaagaa ttcgctggtt tcttcccgac ctggcttgcg
    11641 ccagttcagg ttgttatcat gaatattacc gattcacagt ctgaatacgt taacgaattg
    11701 acgcaaaaac tatcaaatgc gggcattcgt gttaaagcag acttgagaaa tgagaagatt
    11761 ggctttaaaa tccgcgagca cactttgcgt cgcgtcccat atatgctggt ctgtggtgat
    11821 aaagaggtgg aatcaggcaa agttgccgtt cgcacccgcc gtggtaaaga cctgggaagc
    11881 atggacgtaa atgaagtgat cgagaagctg caacaagaga ttcgcagccg cagtcttaaa
    11941 caattggagg aataaggtat taaaggcgga aaacgagttc aaacggcgcg ccctaaccgt
    12001 atcaatggcg aaattcgcgc ccaggaagtt cgcttaacag gtctggaagg cgagcagctt
    12061 ggtattgtga gtctgagaga agctctggag aaagcagaag aagccggagt agacttagtc
    12121 gagatcagcc ctaacgccga gccgccggtt tgtcgtataa tggattacgg caaattcctc
    12181 tatgaaaaga gcaagtcttc taaggaacag aagaaaaagc aaaaagttat ccaggttaag
    12241 gaaattaaat tccgtcctgg tacagatgaa ggcgactatc aggtaaaact ccgcagcctg
    12301 attcgctttc tcgaagaggg tgataaagcc aaaatcacgc tgcgtttccg cggtcgtgag
    12361 atggcgcacc agcaaatcgg tatggaagtg cttaatcgcg tgaaagacga tttgcaagaa
    12421 ctggcagtgg tcgaatcctt cccaacgaag atcgaaggcc gccagatgat catggtgctc
    12481 gctcctaaga agaaacagta aggccttcaa gtagcactgt ctgtggagcc ttcgggcttc
    12541 acaggctttg ttcgcctttg tttcgtttat taacaatgcg aagtggaagt tattaaaatg
    12601 ccaaaaatta agaccgtacg cggtgctgct aagcgcttca aaaaaaccgg taaaggtggt
    12661 tttaagcaca agcacgctaa cctgcgtcac attctgacca aaaaagcgac caaacgtaaa
    12721 cgtcacctgc gtccgaaagc catggtttcc aaaggcgatc tgggcctggt aatcgcgtgc
    12781 ctgccgtacg cataagccgt taacgttttt aactttttaa ttagaatata gatacaggag
    12841 agcacatatg gctcgcgtaa aacgtggtgt tattgcacgc gcacgtcaca agaaaatttt
    12901 gaaacaagct aaaggctact acggtgcgcg ttctcgcgta taccgcgttg ccttccaggc
    12961 tgttatcaaa gctggtcagt atgcttaccg tgaccgtcgt caacgtaagc gtcagttccg
    13021 tcaactgtgg attgcgcgta tcaacgcagc agcacgtcag aacggtattt cttacagcaa
    13081 attcatcaat ggcctgaaaa aagcctctgt tgaaatcgac cgtaagatcc tggctgatat
    13141 cgcagtattc gacaaagtag cgttcaccgc tctggttgaa aaagcgaaag cagctctggc
    13201 ataagccagt tgaaagaggg agctagtctc cctcttttcg tttcaacgcc atcaaaacat
    13261 tgacttttat cgccgtagcc ttttcaataa aggtcttttg aagagtacca aaaggtaacg
    13321 caagcaatga atgctgctat tttccgcttc tttttttact ttagcacctg aatccaggag
    13381 gctagcgcgt gagaagagaa acggaaaaca gcgcctgaaa gcctcccagt ggaggctttt
    13441 tttgtatgcg cgttttgaaa aataaaaagc ctgtctcatc aggtgtaatt gtcgcagcca
    13501 gtctggacac ggccagcgcg gagaaaccgg agcgtattta agtacgtgag aatttcgagc
    13561 actgcccggg accaaaatgg caagtaaaat agcctgatgg gataggctct aagtccaacg
    13621 aaccagtgtc accactgaca caatgaggaa aaccatgtca catctcgcag aactggttgc
    13681 cagtgcgaag gcggccatta gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt
    13741 cgaatatttg ggtaaaaaag ggcacttaac ccttcagatg acgaccctgc gtgagctgcc
    13801 gccagaagag cgtccggcag ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca
    13861 ggcgctgaat gcgcgtaaag cggaactgga aagcgctgca ctgaatgcgc gtctggcggc
    13921 ggaaacgatt gatgtctccc tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt
    13981 tacccgtacc atcgaccgta tcgaaagttt cttcggtgag cttggcttta ccgtggcaac
    14041 cgggccggaa atcgaagacg attatcataa cttcgatgct ctgaacattc ctggtcacca
    14101 cccggcgcgc gctgaccacg acactttctg gtttgacact acccgcctgc tgcgtaccca
    14161 gacctctggc gtacagatcc gcaccatgaa agcccagcag ccaccgattc gtatcatcgc
    14221 gcctggccgc gtttatcgta acgactacga ccagactcac acgccgatgt tccatcagat
    14281 ggaaggtctg attgttgata ccaacatcag ctttaccaac ctgaaaggca ctctgcacga
    14341 tttcctgcgt aacttctttg aggaagattt gcagattcgc ttccgtcctt cctacttccc
    14401 gttcaccgag ccttctgcag aagttgacgt catgggtaaa aacggtaaat ggctggaagt
    14461 gctgggttgc gggatggtgc atccgaacgt gctgcgtaac gttggcatcg acccggaagt
    14521 ttactctggt ttcgccttcg ggatggggat ggagcgtctg actatgttgc gttacggcgt
    14581 caccgacctg cgttcattct tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg
    14641 caggaataga ttatgaaatt cagtgaactg tggttacgcg aatgggtgaa cccggcgatt
    14701 gatagcgatg cgctggcgaa tcaaatcact atggcgggcc tggaagttga cggtgtagaa
    14761 ccggttgccg gtagcttcca cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat
    14821 ccgaacgctg acaaactgcg tgtgacaaaa gtgaatgttg gcggcgatcg cctgctggac
    14881 atcgtctgcg gtgcgccaaa ctgccgtcag ggcctgcgtg tggcggtagc taccattggt
    14941 gctgttctgc cgggtgattt caaaattaaa gcggcgaaac tgcgtggtga accgtctgaa
    15001 gggatgctgt gctccttttc tgagctggga atttctgacg atcatagcgg cattatcgag
    15061 ctgcctgcgg atgcgccgat tggcaccgac atccgtgaat acctgaaact tgatgacaac
    15121 accatcgaaa tcagcgtgac gccaaaccgt gccgactgtt taggtatcat tggtgttgcg
    15181 cgtgacgttg ccgtgctgaa tcagctgccg ctggttgaac cggaaatcgt tccggttggt
    15241 gcgaccatcg acgacacgct gccgattaca gtcgaagcgc cggaagcctg cccgcgttat
    15301 cttggccgtg tggtaaaagg cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa
    15361 aaactgcgtc gttgcgggat ccgttctatc gatgcagttg ttgacgtcac caactatgta
    15421 ctgctcgaac tgggccagcc gatgcacgct ttcgataaag atcgcattga aggcggcatt
    15481 gtggtgcgga tggcgaaaga gggcgaaacg ttggtactgc ttgatggcac tgaagcgaag
    15541 ctgaatgctg acactctggt catcgccgac cacaacaagg cgctggcgat gggcggcatc
    15601 tttggtggcg aacactctgg cgtgaatgac gaaacgcaaa acgtcttgct ggaatgcgct
    15661 ttcttcagcc cgctgtctat caccggtcgt gctcgtcgtc atggcctgca tactgatgcg
    15721 tctcaccgtt atgagcgtgg cgttgatccg gcactgcagc acaaagcgat ggaacgtgcg
    15781 acccgtctgc tgatcgacat ctgcggtggt gaggctggtc cggtaattga tatcaccaac
    15841 gaagcaacgc tgccgaagcg tgcaaccatc actttacgtc gtagcaaact ggatcgcctg
    15901 atcggccatc atattgcgga tgagcaggta actgacattc tgcgtcgtct cggctgcgaa
    15961 gtgaccgaag gcaaagacga gtggcaggca gttgcgccga gctggcgttt cgatatggag
    16021 attgaagaag atctggtcga agaagtcgcg cgtgtttacg gctacaacaa catcccggat
    16081 gagccggtac aggcaagcct gattatgggt actcaccgtg aagctgacct gtcgctcaag
    16141 cgcgtgaaaa cgctgctcaa cgacaaaggc tatcaggaag tgatcaccta cagcttcgtt
    16201 gatccgaaag tgcagcagat gatccatcca ggagttgaag ccttactgct gccaagcccg
    16261 atctctgttg aaatgtcagc aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg
    16321 gtgtacaacc agaaccgtca gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc
    16381 gtaccagata ctcaggcacc gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt
    16441 tgcggtaacc gttacgaaga gcactggaac ctggcaaaag agaccgttga tttctatgat
    16501 ttgaaaggcg atcttgaatc cgttctcgac ctgaccggta aactgaatga ggttgagttc
    16561 cgtgcagaag cgaatccggc actgcatccg gggcaatccg cagcgattta tctgaaaggt
    16621 gaacgtattg gttttgttgg ggttgttcat cctgaactgg aacgtaaact ggatcttaac
    16681 ggtcgcactc tggtgttcga actggagtgg aacaagctcg cagaccgcgt ggtgcctcag
    16741 gcgcgcgaga tttctcgctt cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa
    16801 aacgttcccg cagcggatat tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt
    16861 ggcgtaaact tatttgacgt gtaccgcggt aagggtgttg cggaggggta taagagcctc
    16921 gccataagcc tgatcctgca agataccagc cgtacactcg aagaagagga gattgccgct
    16981 accgtcgcca aatgtgtaga ggcattaaaa gagcgattcc aggcatcatt gagggattga
    17041 acctatggcg cttacaaaag ctgaaatgtc agaatatctg tttgataagc ttgggcttag
    17101 caagcgggat gccaaagaac tggttgaact gtttttcgaa gagatccgtc gcgctctgga
    17161 aaacggcgaa caggtgaaac tctctggttt tggtaacttc gatctgcgtg ataagaatca
    17221 acgcccggga cgtaacccga aaacgggcga ggatattccc attacagcac ggcgcgtggt
    17281 gaccttcaga cccgggcaga agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga
    17341 gtaatctgat ctaactaaaa aggccgctct gcggcctttt ttcttttcac tgtcgaagag
    17401 tcaccgtaaa atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc
    17461 gccaacaaca gcgacaaaat attcgctggt tattatgcct gtcagttttg atgctgctgg
    17521 cgcttctctt aagcctttgc gccggtgaac aatggatctc gccaggtgac tggtttactc
    17581 ctcgtggcga actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg
    17641 ttggtgcggc gctggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg
    17701 cagaacctgg actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat
    17761 tgcttgggca agggcaactc cccaactggg cgctagggct gtgtgcgatt gctggcgcgc
    17821 ttatcatcac tttaatactc ttacgtttcg cccgtcgtca tctttcgacc agtcggttat
    17881 tgctggctgg cgttgcatta gggattatct gtagcgcact aatgacgtgg gctatctact
    17941 tttccacctc tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg
    18001 tagactggcg gcaaagctgg ctgatgctgg cattgatccc cgtgttgttg tggatctgtt
    18061 gtcagtccag gccgatgaat atgttagcac ttggcgagat ctcggcgcgg caactgggtt
    18121 tacccctgtg gttctggcgc aatgtgctgg tggccgcgac cggctggatg gttggcgtca
    18181 gtgtggcgct ggcgggtgct atcggcttta ttggtctggt gatcccacat attcttcggt
    18241 tgtgtggttt aaccgatcat cgcgtattac ttcccggctg cgcgctggca ggggcgagcg
    18301 cattgctgct ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgcctattg
    18361 gcgtggtcac cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaagac
    18421 gttagccgca aaaagacggt ctatgattaa aagctaagat ttttactgac cacacccagg
    18481 agaaacgatg caagattcca ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac
    18541 cacgctggag aagtacgctg gtaatgtgct gttgattgtc aatgtcgcct caaagtgtgg
    18601 cttaacgccg caatatgagc agttggagaa tattcagaaa gcctgggccg atcgaggttt
    18661 tgtggtgctg ggattcccgt gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga
    18721 gattaaaact tactgtacca ccacatgggg ggtgacgttc ccgatgttca gtaagattga
    18781 agttaatggc gaaggacgcc atccgctgta tcaaaaattg attgccgcag cgccgaccgc
    18841 agtcgcgccg gaagagagcg gattctatgc ccgtatggtc agcaaaggtc gtgcaccgct
    18901 gtaccctgat gatattttat ggaattttga gaaattttta gttggcaggg acgggttggt
    18961 catccagcgt ttttccccgg atatgacgcc tgaagatccc attgtgatgg aaagcattaa
    19021 actggcgttg gcaaaataat gtctattgtg atgcagttac aggatgttgc ggaaactacc
    19081 cgcctggggc cactttctgg cgaggttcgg gctggggaga tcctgcacct ggtggggccg
    19141 aatggcgcgg gtaagagtac cttgcttgca cgaatggccg gaatgaccag cggtaaggga
    19201 agcattcagt tcgcggggca accactggaa gcatggtccg caacaaaact cgcgctgcat
    19261 cgcgcctatc tttcacaaca gcagacgccg ccgtttgcaa tgccggtctg gcactacctg
    19321 acactgcatc agcacgataa aacgcgtacc gaactactga atgatgtcgc aggggcgctg
    19381 gctcttgatg acaaactcgg tcgtagcacc aatcaacttt ccggcggtga atggcaacgc
    19441 gtacgtcttg ctgcggtggt gttgcaaatc acaccacaag ccaatcccgc aggccaattg
    19501 ctgcttcttg atgagccgat gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa
    19561 attctgagcg cgctgtgtca gcaaggactg gcgattgtga tgagcagtca cgatctcaac
    19621 cacacgttgc gtcatgcgca tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt
    19681 ggacgcagag aagaggtgct cacgccgcca aatctggcgc aggcctatgg gatgaatttt
    19741 cgccgtctgg atatcgaagg tcacagaatg ctgatttcga ccatctgatt tcattagtat
    19801 cttgaaaatc gggtaattac gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt
    19861 acggaatgcg tttctgcctt attttgatca cagcactgtt tctggccggg tgtagccacc
    19921 ataaagcacc gccgccaaat gccagacttt ctgattcgat taccgttatt gccggtttga
    19981 acgaccagct acaaagctgg catggcacgc cgtatcgtta tggtggcatg acgcggcgcg
    20041 gtgtggactg ttcgggattt gtggttgtga cgatgcgcga tcgtttcgat ttgcagctgc
    20101 cccgtgaaac caaagaacaa gcctctatcg gcacgcaaat tgataaagac gagttgctgc
    20161 ctggtgacct ggtctttttc aaaacgggtt ccggacaaaa tggtttgcat gtaggtattt
    20221 acgataccaa caaccaattt atccacgcct ctaccagcaa gggagtgatg cgttcctcac
    20281 ttgataatgt ctattggcag aaaaatttct ggcaggcgag acgaatctag tatgtcgaaa
    20341 aatggacggc gatgatacat cgccgtccat ttactcctga atacagtagt attttgcaga
    20401 caaaaattcc gtaaatggat tattcgttta taatcaggat aatctaaaaa aaggacccct
    20461 gaaaaagtgg ttaacatgga acaaatgaaa gttactcctt ctgtaattgt tatgtcttat
    20521 ggcctcatcc cttttagccg gatactgaaa aacatccttc gagagggacg gttaccatga
    20581 agattttttt ggagaatctt tatcattctg attgctattt cctgccgata agagataacc
    20641 agcaactcct tgtcggcgtt gagttaatta cccatttctc cagtgaagat ggcacggtgc
    20701 gtatcccgac gagtcgggtt atagcgcaac ttaccgagga gcaacattgg caattatttt
    20761 cagagcagtt ggaattacta aaatcatgcc aacatttttt tattcaacat aaactctttg
    20821 cctggcttaa tttaacgcca caagtggcta cattattatt agagagggat aattacgcag
    20881 gcgaactatt aaaatatccg tttattgaac tgttgattaa tgagaattac ccgcatctca
    20941 atgaaggaaa agataatcgc ggtcttttat ccttatcgca agtgtatccc cttgtgttag
    21001 gtaatttggg agcaggcaat agcacaatga aagcggtttt tgatggtctt ttcacccgcg
    21061 tcatgctgga taaaagtttt attcagcaac aaatcacgca ccgctccttc gaaccgttta
    21121 ttcgtgcgat ccagtcgcaa atttcccctt gttgcaattg catcattgcg ggaggcattg
    21181 atacggcaga aatattagca cagattaccc ccttcgattt ccatgctctc cagggatgcc
    21241 tgtggcctgc cgttcccata aatcagataa cgacgttggt tcagcgataa cccttctgtt
    21301 tgctggtgtt taagacgaga gtaaccgtct acactatcaa acaggaggat ctatgaccct
    21361 gtcttttatt acccgctggc gcgatgaatt gccagaaacc tatacagcac tttcccctac
    21421 gcctttaaat aatgcccggc tgatttggca taataccgaa ctcgctaaca cgctgagtat
    21481 tccatcgtcg ctgtttaaaa atgctgcagg tgtctggggc ggcgaaaccc tactgcctgg
    21541 tatgtcacca ctggcgcagg tttacagtgg tcatcagttc ggcgtctggg cgggccaact
    21601 gggtgatggg cgcggcattt tacttggcga acaactgctt gctgatggca ctacaatgga
    21661 ctggcatctg aaaggtgctg gcctgacgcc ttattcgcga atgggtgatg gacgggcggt
    21721 tttacgttcg acgatacgag aaagtctcgc cagtgaggcg atgcattatc tgggcattcc
    21781 gacgacccgc gcgttaagta tcgtcaccag cgattcgcca gtgtatcggg aaacggtgga
    21841 gccaggcgcg atgctgatac gtgtggcacc aagtcatctg cgctttggtc atttcgaaca
    21901 tttttactat cgccgcgagc cggaaaaggt tcgtcagtta gctgactttg ccattcgtca
    21961 ttactggtca catcttgaag atgatgagga caaataccgt ctctggttta acgatgttgt
    22021 cgcacgtacc gcatcgttaa ttgcccaatg gcagacggtc ggctttgctc atggggtgat
    22081 gaataccgac aacatgtcgc tgctggggct gacgcttgat tacgggccgt ttggttttct
    22141 tgatgattac gaacccggtt ttatttgtaa tcactcggat catcaagggc gttacagctt
    22201 tgataatcaa cctgccgtcg cgttgtggaa tttacagcgt ctggcgcaga cattgtcacc
    22261 atttgttgcc gtagatgccc tgaatgaggc cctggacagc tatcagcagg ttttgttgac
    22321 gcattatgga caacggatgc ggcagaaact gggcttcatg acggagcaaa aagaggataa
    22381 cgcgctactg aatgaattat tcagtctgat ggcgcgagag cgcagcgatt atacccgcac
    22441 attccgcatg ctgagtctga ccgagcagca cagcgcggcg tcaccgctac gtgatgagtt
    22501 tattgatcgt gcggcatttg atgactggtt tgcccgttat cggaggcgtt tgcaacaaga
    22561 cgaggttagc gatattgagc gtcagcaact gatgcaaagc gttaacccag ctctggtgtt
    22621 gcgcaactgg ttggcgcaac gggcgattga agcggcagaa aagggtgata tgacggaatt
    22681 gcaccgcctg catgaggcgt tgcgaaatcc tttcagcgac agagctgatg actatgtcag
    22741 tcgtccacct gactggggta aacggctgga agtcagctgc tcgagttaaa ggctacttag
    22801 ttaacagcag cttgccagcc tgagttttac ggagcagata ttcttgcccg tcatgatcga
    22861 taatcagttt gccatccgga cctaacagtg tctggctgga aattcgccga ataggctgcg
    22921 gggaagcggt tttgtgattg gcatccgttt caggcgtgag ttttctgcta tccgtataac
    22981 gcataaaaat taataataat tgatataaca atgattctta ttatcaatat tatggctgac
    23041 cagagcaagc agtaatgtgc tgagaagaca acaagttgcg cagaaatgcc catcccatca
    23101 ggaatgggca cgcattcaga agcgggtatc taccgcagag gcaagttttt cgaccaggcg
    23161 ttcggtatct tcccagccca gacacgggtc ggtaatggat tgaccgtaag tgagcggctg
    23221 accgccgacg attttttgcg ttccttcgcg caggaaactt tccgccataa ttccagcaat
    23281 cgccgtagag ccattgcgga tttgctgaca aatatcctca caaacttcta actggcgacg
    23341 gtgctgcttc tggcagttac cgtggctgaa atccaccacc agatgttcag gtaaatcaaa
    23401 ctcgtgcagc gtatcgcagg ctgcggcgat atcatcggca tgataattcg gttttttgcc
    23461 gccacgcata ataatgtggc catacgggtt gccgctggtc tgatagatgg tcatctgacc
    23521 atttttgtct ggcgagagaa acatatggct ggcgcgggct gcgcggatag catccacggc
    23581 aatccgcgtg ttgccatcgg taccattttt aaaacctacc ggacaggaga gtgccgaagc
    23641 catttcacgg tggatctgac tttcggtagt acgtgcgcca atcgcgcccc aactgattaa
    23701 atcagcaata aactgaccgg tcaccatatc gaggaactca gtcgcggtag ggacacccag
    23761 ctcatttacc tgtaaaagta atttgcgcgc cagctccaga ccgtgattta cccgatagct
    23821 gccgtttaaa tccggatcgg agattagccc tttccagccg acaacagttc gtggtttttc
    23881 aaaataggtg cgcattacga tttccagccg tgactggtac tggttgcgca gcgactgcag
    23941 acgggtggcg tactccattg cagcggtgag atcgtggatc gagcaggggc caataataac
    24001 caacagtcgc ttatcttcac cattcagtat tttttcaatt ctgcggcggg agtcggtgac
    24061 atgggtggcg acgccaggcg ttacgggata ccgtagcgcg agttcggcgg gcgttaccag
    24121 gctctcaata cgcgcagtac ggagttcgtc agttctgttc attacaagtc tcagaaattt
    24181 gttgcttcta cggcaggact accgggaagt gatgcgcatc acgataaacc aatcgcagct
    24241 gatttcaagt gcgaggtgtg tcacacctcg ccgggtggtt agcatgataa caaaaaaata
    24301 agctaatgca ctagttctct agtacattcg gcggctaagc cccatgatat cgaggatctt
    24361 ggtggcaatc tcttctaccg aataattggt actgttaatc cacgggatct gatttttacg
    24421 gtacaacgct tctacttccg cgacttccat cctgcactga cgaagcgagg catagcggct
    24481 gttctcccga cgttcctcgc gaatcgccgc cagacgttcc gggtcgatag tcaggccgaa
    24541 caatttatgc tgaagcggtt tgagcgacgc gggtagcacc agattatcca tatcgtcggc
    24601 aataaagggg tagtttgccg cgcggatacc aaattgcatt gccagataca gactggtggg
    24661 ggttttacca cagcgagaaa cgccgagcag gatcacctga gcctggtcca gattgcgcaa
    24721 cgaaataccg tcatcgtggg caagggtgta atcaatcgcc gcaatgcgcg catcatattt
    24781 attgagatta ttagggttaa ggccatgggt acgatgagca atcggcgttg gatccagttt
    24841 catctcttgt tgtagcgggg caaccagcgc ctgaacgata tcctggcaaa agccttcact
    24901 ttgcaagatg atggcgcgaa tctccggcaa cacgatggag tagaagacca gcgggcgcac
    24961 acctgtctgg tgataaattg cgtcaatctg atccttcact gcccgtgcac ggctctcatt
    25021 ttcgacaaac ggcagcgtaa tgctgctgat agtgacggga aattgtgaca ttactgcgtg
    25081 tcctaatacc tccgcagtta ttgccgtacc atcagaaata taaaaaacgt ggcgatcaac
    25141 agcattatcc attttgttct tcccgtgatg cagacataat tctatgaaag cataaattaa
    25201 aaacgcacag aagcgtagaa cgttatgtct ggtttataaa atgaaccttc aattttattt
    25261 tttatgaaaa cagcatttca tttttatggt ttcgtttata ccgatggttt atgtggaaat
    25321 tgtcgaagag agcagatttg cgcaacgctg ggatcagtct taaaaagtaa aaaaatatat
    25381 ttgcttgaac gattcaccgt ttttttcatc cggttaaata tgcaaagata aatgcgcaga
    25441 aatgtgtttc tcaaaccgtt catttatcac aaaaggattg ttcgatgtcc aacaatggct
    25501 cgtcaccgct ggtgctttgg tataaccaac tcggcatgaa tgatgtagac agggttgggg
    25561 gcaaaaatgc ctccctgggt gaaatgatta ctaacctttc cggaatgggt gtttccgttc
    25621 cgaatggttt cgccacaacc gccgacgcgt ttaaccagtt tctggaccaa agcggcgtaa
    25681 accagcgcat ttatgaactg ctggataaaa cggatattga cgatgttact cagcttgcga
    25741 aagcgggcgc gcaaatccgc cagtggatta tcgacactcc cttccagcct gagctggaaa
    25801 acgccatccg cgaagcctat gcacagcttt ccgccgatga cgaaaacgcc tcttttgcgg
    25861 tgcgctcctc cgccaccgca gaagatatgc cggacgcttc ctttgccggt cagcaggaaa
    25921 ccttcctcaa cgttcagggg tttgacgccg ttctcgtggc agtgaaacat gtatttgctt
    25981 ctctgtttaa cgatcgcgcc atctcttatc gtgtgcacca gggttacgat caccgtggcg
    26041 tggcgctctc cgccggtgtt caacggatgg tgcgctctga cctcgcatca tctggcgtta
    26101 tgttctccat tgataccgaa tccggctttg accaggtggt gtttatcact tccgcatggg
    26161 gccttgggga aatggtcgtg cagggtgcgg ttaacccgga tgagttttac gtccataaac
    26221 cgacactggc ggcgaatcgc ccggctatcg tgcgccgcac catggggtcg aaaaaaatcc
    26281 gcatggttta cgcgccgacc caggagcacg gcaagcaggt taaaatcgaa gacgtaccgc
    26341 aggaacagcg tgacatcttc tcgctgacca acgaagaagt gcaggaactg gcaaaacagg
    26401 ccgtacaaat tgagaaacac tacggtcgcc caatggatat tgagtgggcg aaagatggcc
    26461 acaccggtaa actgttcatt gtgcaggcgc gtccggaaac cgtgcgctca cgcggtcagg
    26521 tcatggagcg ttatacgctg cattcacagg gtaagattat cgccgaaggc cgtgctatcg
    26581 gtcatcgcat cggtgcgggt ccggtgaaag tcatccatga catcagcgaa atgaaccgca
    26641 tcgaacctgg tgacgtgctg gtcactgaca tgaccgaccc ggactgggaa ccgatcatga
    26701 agaaagcatc tgccatcgtc accaaccgtg gcggtcgtac ctgtcacgcg gcgatcatcg
    26761 ctcgtgaact gggtattccg gcggtagtgg gctgtggtga tgcaacagaa cggatgaaag
    26821 acggtgagaa cgtcactgtt tcttgtgccg aaggtgatac cggttacgtc tatgcggagt
    26881 tgctggaatt tagcgtgaaa agctccagcg tagaaaccat gccggatctg ccgttgaaag
    26941 tgatgatgaa cgtcggtaac ccggatcgag ctttcgactt cgcctgtctg ccgaacgaag
    27001 gcgtgggcct tgcgcgtctg gaatttatca tcaaccgtat gattggcgtc cacccacgcg
    27061 cactgcttga gtttgacgat caggaaccgc agttgcaaaa cgaaatccgc gagatgatga
    27121 aaggttttga ttctccgcgt gaattttacg ttggtcgtct gactgaaggg atcgcgacgc
    27181 tgggtgccgc gttttatccg aagcgcgtca ttgtccgtct ctctgatttt aaatcgaacg
    27241 aatatgccaa cctggtcggt ggtgagcgtt acgagccaga tgaagagaac ccgatgctcg
    27301 gcttccgtgg cgcggggcgc tatgtttccg acagcttccg cgactgtttc gcgctggagt
    27361 gtgaagcagt gaaacgtgtg cgcaacgata tggggctgac caacgttgag atcatgatcc
    27421 cgttcgtgcg taccgtagat caggcgaaag cggtggttga ggaactggcg cgtcaggggc
    27481 tgaagcgtgg tgagaacggg ctgaaaatca tcatgatgtg tgaaatcccg tccaacgcct
    27541 tgctggccga gcagttcctc gaatatttcg acggcttctc aattggctca aacgatatga
    27601 cgcagctggc gctcggtctg gaccgtgact ccggcgtggt gtctgaattg ttcgatgagc
    27661 gcaacgatgc ggtgaaagca ctgctgtcga tggcgattcg tgccgcgaag aaacagggca
    27721 aatatgtcgg gatttgcggt cagggtccgt ccgaccacga agactttgcc gcatggttga
    27781 tggaagaggg gatcgatagc ctgtctctga acccggacac cgtggtgcaa acttggttaa
    27841 gcctggctga actgaagaaa taaaataaat ccccggcggc gttttgtcgc cgggtttatg
    27901 tgatccccga agatgaaact tattcaatct cttcacagac atcctgagtt aatcgccgca
    27961 taatatcttt ccttaacaaa aacttttgta ttttacctga ggcagtgcgc ggtagttttt
    28021 cgattaccac gatatgttca ggatatttat attttgcgac ccgtttacgg ctaaaaaaag
    28081 ccaccacctc ttccagcgat aatgaatgat gcggcgcttt cagcacgaca taagcgcatg
    28141 atcgttcacc taaacgttca tcgggcattg caaccacaca ggcatcgtga attttaggat
    28201 gctgcaataa aatatcttcc acttcacggc tgctaatatt ttcgccgccg cggacaataa
    28261 tatctttttt gcgtccggta atttttatat agccagcctc atccatacgg cagagatcgc
    28321 cgctgtaata ccagccttct tcatccagcg cacgggcggt taattcaggt tcatcaaaat
    28381 atcccataaa cacattgggg ccacgcgagg cttcttcacc ttcgcaacct ggcggtaagg
    28441 tcttgcgtgc gtcatcgacc actttaatct ctacacctgc ggcagcgtaa ccatcggtgt
    28501 gcataaagcg cgacaaagga tcatcgagat tcaccaccgc atgcggcgaa ctttctgtgg
    28561 aaccataaac acttaataat ttaatgccgc gctgctggca ttcacgcgcc acttttttgg
    28621 ggattgtggt accgccgcaa agaaagaagc gcagcgctga aaggtctgcg ggttgtttct
    28681 ctagtaaatt caaaagatca tagacaaacg gcgttgcgcc gagcatacag gtgcaacgct
    28741 gctgctcaag cagcgcgaga cacgcatcag gagtgaaaat atctaacaac acgctgcgag
    28801 caccaatcaa aaatggtgct gttacgccat gcagaaagcc cgttgcgtgg ccgagtggcg
    28861 caggcatcat aaagacatcc tgccaggtca gattcagccg cgcgcaataa gcccgctcac
    28921 tggcgagaat attgttatgc gttagcatca cgccctttgg cagaccctcg gttccggagg
    28981 taaacagcac cgcagctaat tcatcgccgt gggtcgttat cgccgtggtc agtggggtat
    29041 tgtcggcgat aatctgactt aatgagaggg aagaggtggc gggagccagt ttgtccacgc
    29101 cgacaatttg ttgtagttgt ggaagctgat tttgcagcgg caggattaaa tctaccggac
    29161 gcgtttgttt aaacaacgtc ggtgcaaaga acatttttgc ctgacactta ttgagcaccc
    29221 ataccagttc tgcttcccgc caggaaggca acagcggtac ggaaaccgca ccgattttca
    29281 ggcatgcaag atagataaca gtaaattcac accagccagg cagttgaaat gcgatgcgat
    29341 cgcctgattc aatacccttc gctaacatcc agtttgccag acagctcgcg gcgtgatcga
    29401 gcgcgctata ggtgtacgat gctccatgat tatcgaccac ggcaattttg tctggcatcg
    29461 cacgagcggt ctgctgccag taatcggcca gcgaagcatc gccccatagc ccttgctgac
    29521 gatacgccgc acgacgttgt tcgttgaacg ttaatgtcac tttcatgttg gactctcgaa
    29581 acagaacatc aggcccgaga tgcgggcctg tgggatgcat gattagccgt agcgaaaatc
    29641 aataccgaag gtgcctgcgg gatattgcca ctgttcgaga atagtgttac cgcacagcac
    29701 ccgacaggtg ccgcactcca gacaaccggc agaatcaaaa tgaatgttgc cggcgtcatc
    29761 ctgcttataa agtccggcag ggcaggcttt cattaattta cggaattcat tgatatcggg
    29821 atttgccgcc agaatgatat gcgggtggcc ctcatcaaca tggaatttat tgacgcctaa
    29881 tttgatgtcg acgttaaccg tagcgttctg gctcatagcg cggttgctcc cttaatgcca
    29941 tctttcagca agttgatcag cccaattttc ttcgcatgtc ccatgatcat tttgcgtacc
    30001 ggctggtttg gtttgccgtc aatggtgaac atctcgttca tgatgtcggc gaccattcgt
    30061 gggtattggc taaacaggcg cgggttttct atcagcgccg ggatcttgcg gaaatgctgc
    30121 atatcgcgca tgacgcagct ttgttccagc tcacgtttgt attgcgccag actgctggcg
    30181 gagaaatcct cgcgttcttt ggcggcgatc accgttgtgg cggcagcctg agccgatgca
    30241 atggctaaat ccatgccacg aactgtaaaa cccaaattca ggcagaagcc tgcggcgtca
    30301 ccaacgatca tcacgccatc gttaaccatc tgcggcacca ttgccagacc gccttctggc
    30361 accatatgcg cggaatattc aagcagtttg ccgccgctaa ttagtgggcg aatggcgggg
    30421 tgttgtttaa aatcttccag catttgcggc acgctttttt gcgcatgggc gatatcaccc
    30481 aatccacaaa ccagccccaa ggatatggaa tccttgttgg tatagagaaa tcccccgccc
    30541 atcaggccgt cagaagggga accggcaaac agccaggcgg caccttcctc gcccgtaata
    30601 ttaaagcgat cgttgatctg ttctggtgtg aggccaataa cctctttaac accaacagcg
    30661 taatgatgcg gatcggaagc gggaaccatt cccagcgagc ggccaagcat cgagttaacg
    30721 ccatcagcca gaatcaccac attcgcttcg agaatatcat ccccagcctg cacgccagtg
    30781 accttgtttc cttcacgaac cagcgcatcg acgcgaacgc ctgggataaa ctgcgcgccc
    30841 gcctgctcgg cttgttccat caaccacggg tccagacgat tacgcaatac ggtataagat
    30901 gcgtgttgcg gaacatctgg tttctcgcgg tgaaaatcga gggtaacggc gctttcttcg
    30961 gttaagaagg agattttctc gcgtgtgacc ttgcgttcta ccggcgctga tactgcaaag
    31021 cctggaatga ttgcttcaag tgtgtgggca taaagacgcc cgccggtcat gtttttacat
    31081 ccggcactgt cgccgcgttc tatcaccagg acatccagcc cggctcgcgc catgaccagt
    31141 gcggcaacgc taccagcaac gcccgcaccg accacaatgg catcaaattt gtcttccgac
    31201 atgcgttatg ctcccttccc gctgcgttta cagcgggaga tgtggttgaa aggggaatta
    31261 gcggctcaac tggctaatca gggcagggac gactttgtag atatcgccca ccagaccgta
    31321 gtcggcataa ttgaagattg gcgcattttt atctttattg atggcgacaa tcacttttgc
    31381 gccgttgccg ccaaccatat gctggatctg cccggagatc cccagcgtca ggtagagatc
    31441 ggatttcagc aacacgccgg agacaccgat ataacgttca cgctccatcc agttctcgcc
    31501 ttcggcaatt ggacgtgaac agccgacttc agcattcagc accgccgcca gttcgtggac
    31561 cattttcagg tcatcctgcg ccgccagacc acgaccgacg ccaaccacac gttttgcttt
    31621 gctcaggtcc acgctgcttg cggctttcgc acggcgttcg cgacagagaa tttcatgacg
    31681 cggagcaaca taaggtaccg tttctgtcgg gcactgatga gaggtatcac tggtgcacgg
    31741 ttcctgaaca ccgggtgcaa gggtaatgat cgccagcggg ctgttgatct tttcctgagc
    31801 gaacgctaac ccgccataca tccggtgttc ggcgcaaatg tgaccatcga caatatccac
    31861 cgccgtggca tcgttcacca gcgccgcatt cagttgcaca cttaaccgtg ctgccagagc
    31921 tttaccacgt ttcgtcgcgg ccaacagcaa catagcgggg tgtttatctt tcagcagggc
    31981 agcaatgctt tcggcgtaat tttcagtgcg ttgcagcgcg tcgttttgct caagaacata
    32041 aatacatttt ggaccataag gcataactgc ctgcgcctgg tcggtatttt gtacaatggc
    32101 atacacctgt tggccccatt gctgagcgcc gccaaacagt tcagcataac gttcaggatt
    32161 atcgctaaag acccagacgc tgtttaattg actcatcgtt tcttctccgt aggcttcagt
    32221 tcagggcttt cttcagatgt tccgccagct cggcaatggc ctccggagaa tcgttatcga
    32281 taatgatgtg cttacgttct gtttgcggcg gtacgcgaat gccgaccagt tcagcaagtg
    32341 gcgcgctctg gctccagtca atatcacttg cctgccactg atttaccggt tttttacccg
    32401 cgccgagaat ggctttcatc gaaggaatgc gtggcacgtt aatatcggag gtgacgcaga
    32461 gcacggctgg aacagagagt tcaataactt caacatcatc ttcaagcgtg cgttcaatca
    32521 ccagtgtatt gccctgacgc tgaatagcac tcactgcatt aatcaccgga agttgcagaa
    32581 tttctccgac cagcaagcca acctgctggg cataaaggtc gccggaacct tcaccaaaga
    32641 tcagtaaatc gaagccgatc ttttcaattg ctgccgccag cgcttttgcg gtatcgagag
    32701 gcagtgcatg ttcaagttgc gcatcctgca ccaaatacag gctgtgcggc ccgcgggata
    32761 gcacgtcttt gcgcactttc gagttctgca acaatgagcc gccaacggtc agcgcggcta
    32821 tctcatcgtc atctgttgcg agctggcttg cagcttcaat ggcattgaga tcgaactggc
    32881 tgattttggc gtcggcattg tcgaaattca gggtgtattc tggagtgaca acaatgtcct
    32941 gttcttcagg caccagctta aagcaggtta ttattttcat gatttctcct gtgagtcgca
    33001 tacggaacca tctccgatgg aaggtaaaaa tggcctgaaa atagtgtgcg atagcggtgc
    33061 ggggttttcg acggagcaag ctgttttcct tgccaacaat cttgcaaaaa aaatgcgatc
    33121 gctaaaacaa aagtgcaatt tgcggcaata tagttattcc atgaccgtac aatcttgcat
    33181 tcagtgtaaa taatcttgtt tagctctcgc taaacgcggt tgtataaccg caatacacgc
    33241 cgtattttat ccggcatata ttgtttttgc ttgctcatgg aatagggagg ttgtcatgta
    33301 tcaacgctgt tttgataacg ccagtgaaac gctgtttgtc gccggtaaaa cgccacggct
    33361 ttcacgtttt gcatttagcg atgatccaaa atgggagtcc ggacatcacg ttcatgacaa
    33421 tgaaaccgag ttgatttacg tcaagaaagg ggttgcaagg tttaccatcg attcttcgtt
    33481 atatgtcgca catgcggatg acattgtggt gatagaacgc ggcaggctgc atgcggtggc
    33541 ctctgacgtt aacgatccgg caacgacgtg tacctgtgcg ctgtacggct ttcagtttca
    33601 gggggctgag gaaaatcagc tactgcaacc gcattcttgt ccggtaattg ccgcggggca
    33661 gggaaaagaa gtcataaaaa ccttatttaa tgagctaagt gtgattttgc cgcaaagtaa
    33721 aaatagccaa acatcttcgt tatgggatgc atttgcctat acgttagcca ttctttacta
    33781 cgaaaatttt aaaaatgctt atcgttcgga gcaggggtat attaaaaaag atgttctgat
    33841 aaaagatatt cttttctatc tgaataataa ttatcgcgaa aaaatcactt tagaacagtt
    33901 atcgaaaaaa tttcgtgcca gcgtcagtta tatttgccat gaatttacca aagagtatcg
    33961 tatttcccct attaactatg ttattcaacg gcgtatgacg gaagcaaaat ggtcactcac
    34021 taatactgaa ttatcacagg cagagatctc ctgacgtgtg ggttatgaaa atgtcgatca
    34081 ctttgccaaa ctgtttttgc gccatgtcgg ttgttcgccc agcgattacc gtagacaatt
    34141 taaaaactgt tttgcggaac aagaaatcct atctgaattt cctcaaccgg taagtcttgt
    34201 tggataaatc tggcggcgca gtttgttaag aagctgcgcc gcctgcagat tatttgttct
    34261 gatagtcttt caggatctgc cgacctgcta cgtaaatcat aatttcgtct gtaccgccgc
    34321 cgatacgttc acaacggaca tcacgccaga agcgggagac gcgcgcttca tcggtatagc
    34381 ccagaccgcc catgatttga atcgcatcat caatgacttc cattgcggta cgtgcgcaat
    34441 acagttttgc cagcgccgcg ctggtgcgca gtgactgatg ctgatcggct tgccatgcca
    34501 ctttcagcac catgttgcgc atgttgtcga tcttaatcgc catcagcgcc agtttttcct
    34561 ggatcatctg gttatgacca atgggcttac caaaagcgat acgttggttg gcatagcggg
    34621 cggcatcttc aaaggtgcat tcggcaaagc cggtgctgcg cgcggcgttg atcaggcgct
    34681 ccatctcaaa gttgtacatc acattgagga aacccattcc ttcttcgccc accatgtcgc
    34741 tctcttcaac ctccacgttg tcgagataga cttcgcaggt gctgagcata tgccagccga
    34801 ttttatgcag cgggttaatc ttaatgccgg gcttactgga gtcgacccac cacagggtga
    34861 aggctttttt gggatctttc ggttgcggat cgcgcgccaa caccagcata tacgggtact
    34921 ctttcgcacc ggtaataaag gttttttgtc cgttgatgta gaccttgccg tttttacgcg
    34981 tgtaagtggt agtggcactg ttgttatctg agccagcacc tggttccgtc aacgccaggg
    35041 cataggcggg atcacccgtt tccagggtgc tttctgccgt tttacgtagc tgctctgcag
    35101 aaccgaaacg gcgcatactg tgaatacatt gaccgttggt gatcaaaaat gccggagcac
    35161 cgcattttga cacttccatc agcgccagca tttgggtgac gtaatccgca gggataccac
    35221 caaattcttc cggcacgcca agcatggaaa taccgttatc cgccagcgcc cgcataaact
    35281 cacgcggata tgtcccgttt tgatcgcagg tgcggaaata ctcttccgga aagttagtcg
    35341 taatcagttc gcgaatactg gccagcagca gttcttgttc ttcagttaaa gaaaaatcca
    35401 tcattatact cctgttaatg agccgcttca ggcaggacaa aacccatcgc cgcatcgata
    35461 aataatcttt cgttcatcag tttgagttct ggcgaaatca ctggggtgaa gtccattttg
    35521 tcgagaatat ctttttgtaa atcgacgcca ggggcgattt cgattaaatg caggccgtct
    35581 tctttcagcg tgaatacagc acgttcggtg atgtaacgaa catccagtcc ccgttcgagc
    35641 gcgatttttc cgctaaaagt aatttccggt agttcccgaa taaatttatt tacccgtcct
    35701 tcctggacga tatgcaattt gccatcggca atttctgttt ttaaactgcc cgcagttaac
    35761 gtgccgcaga aaataatttt cttcgaggtg gcactgatat caataaatcc accggtaccc
    35821 atgattttgc cattgaattt atgcacgccg acgttaccgt gctggtcgac ttcagcaaaa
    35881 ctcaaataac aaacatccag accaccaccg tgataaaaat caaactggga cgtcatatcc
    35941 agaatggcgc gggtattcac gttcgcgcca aaggcgatac cctgtgaagt aattccgcca
    36001 atcggacctg tttctaccgt cagaataaag tcatcagcac aaccttcttc ccgggcgacc
    36061 aggccaatac cgtcagcaat accgacgccg acattcccca ccgcgccttt acgcatttcg
    36121 aataatgcac gccgcgcaac taatttacgt tggtttaggg gcaggctaag tttggtgctg
    36181 tcatcaaggg tgaagtcacc agaaataaaa cggttaaccg gcgcaccgcc atacagttgg
    36241 ctttgatccg gatcgaccac cacaatatcc accagataac ccggaatacg gacagattta
    36301 ggatgcagcg tggctttctt aaccattttc tgcacctgca tcatcacaat accgccgtta
    36361 ttgtgcaccg cctgggcaat aaccaatgcg tcgagataca tcacctcatc ttcaaaagtg
    36421 gcgtagcctt cactgtcgca ggtggtagcg cgaatgaagg cgatatctgg cgcaatcgct
    36481 ttgtaataga gatattcttt gttatcaaac tcgaccagtt taatcaggtc ttctttcgtg
    36541 acttcattca gtttgccgcc ttgctggcgt ggatcgacaa atgtcccgat gccaatatca
    36601 ctaataatac caggctggtg ggctgcggcg gcgcgtaagg tttgtgtaag tacaccttgt
    36661 ggatagttat aagcaataat tttattttgt tctgcgagtt cagaaatacg cggcgattgt
    36721 ccccagtgac cacataatgc ccatttcacc agaccttctt gcgccagagg actaatacca
    36781 cggtcggcgc gatcgccaag ccctgtcgga ctaataatcg ataaattacg tggcgtttga
    36841 gtctgtttat atttatcagc aagagctgta attaacgtgg tggcttccag aataccgccg
    36901 ccagcgccta acacacaaag tgttgcttcg tcgggaatat aattcaccgc ttcctgtgcc
    36961 gacaggaccg gcactcgtcc attaatacga ggtggtttta caggtttcat tgttaattcc
    37021 tgattcagat gtgattttcc ctacgccaga tggagaatta gcgcacagag actcacgaaa
    37081 tatttttatt aaatacggtt aaatatattt tttaagttcg gcggggaggg tgttcccgcc
    37141 gaaatattat tgcttatgcc tggtgtaaaa tagttaatac cgtgcgtaaa tcatttaccg
    37201 agatttgccc tggtgcagag gcttttttta ccgcaccaaa agttgccgcc gagccaaata
    37261 cttcaccagc cagacgagaa attacgccag tttttgccat cgacatcgtg atgattggac
    37321 gatcggcata ctgctcctgc atctccaggg tcgcggtaag caacgtcagc acatcgctgg
    37381 tactttgcgg catcagcgca atcttaggaa tatcggcgtc gaaggactgc attttgcgca
    37441 gacgggcaat gatttcttcg gcttccggcg ttttatggaa gtcatggttg gacatgacaa
    37501 ctttcacatc atgcgcgtgg gcgtaggcga cggtttcttt aacctgatca tcgccggtaa
    37561 ataactccag atcgatcata tcaaccaggc cgctgtcgat ggctgcacga ttgagagcaa
    37621 tataagcctc ggtggaaatc gcctgctcgc cgccttcttt ggcactgcgg aaggtaaaca
    37681 gcagcggttt ttctggcatg gtttcacgga gaatttttgc cgccgccatg acagactcca
    37741 cattggagag gtcggcaaag tggtccacac gccattccag aatatcaaag tccgcttcac
    37801 gataggcgag agcttcggat ttcacgcggg cgatatcttt cgccatcagc gagacgatga
    37861 ttttaggtgc gcccgtacca atgacgagat cttttacagt tacggttttc attttttacc
    37921 ctttctgcac gcggtcagcc tgtcaggcac cgaaccccat gacctgttta acatattcca
    37981 gagggaaatc tttgccagtc cataacgtga actgttcagc cccttgccac aacaacatgc
    38041 cgtatccatc aatcgttttg caaccagctt gttgcgcctg ctgcaataac ttcgtcatat
    38101 gcgggttata cacgcattca gtgaccagca gtcccggatg taacagactg atatcattaa
    38161 ctaatgattc attctcaagg ggtttcatac ccacttttgt gccattggtt aaaatgtcgg
    38221 cggaagccag ggcttcagca aaggcttgct gatcggcgag atcggtaacc gtgacgacac
    38281 aatcggtgtt ttcgttaacc cgctgcgcga aggcgagggc tttatcgaag aactcatccc
    38341 gacggttaaa gagtttaatt tcttttaaac cttcaattgc cccctgcgcg ccaatcgccg
    38401 ttgaggcacc tccggcccct aacagcacca ttgttttgcc tttgatatca aaaccgctct
    38461 ctttaatggc gcgaatatgg cccgtgccgt cggtgttata gccgcgcagg tagccatcat
    38521 cattaacgat ggtgttgatg gcacccacca gtttggctgc tggcgttaat tcatcaacat
    38581 attcacacgc cagttgtttg ttcggcattg atataccagt tccgcgcatt ttgagggctt
    38641 ttaatccttc aattgctccg ggaaaactat cgttatccac ttcaaaggcc atataggtaa
    38701 atggcaatcc cgctttttct aaggctttat tctgcatttc aggcgataaa ctgtggcgga
    38761 taggataggc catcagccca atcaattcgt attttgcggt aacatccata attaactcct
    38821 ttaacctcta tgcttaattg tgtttaaccg ggtgctaaat ttacgctcgc caaaccgcac
    38881 atcattctca ggaataataa aaacgcggta atagcggata aaaacaataa ttgcggtaat
    38941 cagggccagc agcgcgaaag taaaatcgag aacaatgata tattgaaggc cgatgttcga
    39001 cagataaccg gtaatcagcg gaataacaaa gttagccagt ccacccatca tcatataaat
    39061 actggtgact ttggctttgc tttttgggaa aaactctgac atgaccgaaa caccgagctg
    39121 taaaatgccg ccagctgctg agaaaccgat aacaaaggcg ccggcattac acaccagtgg
    39181 agaagggtac aggtagataa tggctgctgt tattgttgcc agtgcagagt taaatacatt
    39241 agcccagatg ggccggatca tttttttcag tagtgcggca aaaataaaga cacagaccaa
    39301 cgagcccata ctgtaataag agatggtttt taatgcctca gcttctgaca taccagcaaa
    39361 agccatcgca tatttgggca tccacaccac aatcacataa aaggtcgaga atgcggctac
    39421 accgaacagt accgatgaaa caccttccag ccagactaac ggtttgctgt tcatttgcgg
    39481 taattcatta gctacgctgg cgtccaccaa ctggctgggg aatttgcttt tcaacagcat
    39541 cagcgtgatc agtacaaaca gaatacccgg aataatcagc ccatagccgt accagatatt
    39601 attgagcaac atatagctca ccagcattgg gtagaacatt tgcccaaatg acaccatcgc
    39661 tttaaccagt atgaccgccg aaccagaggc tttcggaaag cattccatga gcgcggggta
    39721 gccacccgta tccagcgctg agttagcgat acctacgcac actgctagac cgtaggcgag
    39781 agttaaattc gggcaagcgg gaataccaaa gaagaatagc agatacatta ttactgccat
    39841 taatatcacc gcccgacgac caaacttatc ggagatcaca ccgaagaata aaatactgat
    39901 caatcgcccc aaaccgatac cggaaattaa gtaggcaatg cccgcgttgt cagtggaaaa
    39961 cttttctgcc agagatgaca tattttgggc aagcgtaata acactaatgc cgtgcaggaa
    40021 atagctgaag taaatacaaa gaacagccag gataaatggc gtgctgaaag ccttattttg
    40081 agacattttc acagctcctt gctgaaagat aatttcgtta ccgtacgtat tgttctccga
    40141 cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca taaagtttat
    40201 gtttccagga atattgcatg tcatagtttg tttttgatcg cgattttcct tagttagcgc
    40261 agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg cattacccac
    40321 ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg cgtaagccga
    40381 taaacagtgc cagtaaaaag ccgatggcag cgatggcggt atcgaaccac ataatatcgg
    40441 caatacttct ttgggacaga tgagccgtaa tcaacggaat agtaaaggtc gcaatactgc
    40501 ccgcactgta atagatccct gtagctttac ctttagcgta ggggaaacgt tcagccatta
    40561 acgtcaggcc aatttgcaca acacctccag cagaggtaaa accaatgaca aaagcaaata
    40621 ttatcaccac ataaaatgtg ggatgcaggc agacggtaaa cagagcaata aatgagataa
    40681 aggtgtacag catcagtaat gttgttgggc gaacggtatt tcgaatgagt ggagcggtaa
    40741 taaatacaca aagcagcgaa cccacggtgt agatactgag tagtttgatc gacatagtgt
    40801 atgacatgcc tgcaacaaat tgtccgtact gtgacagcca ctggctaacc agataaaacg
    40861 ttgccattga gatatagcca tataaggtat aactggctaa atcgataatt gaacagcgat
    40921 gttccgtaga gcgggtggtt ttctttatga caggtaagcg acgacccgga tggggtggga
    40981 acgtacaacg gtataaaaac agagcgttaa taagcataat gcctgcagca atcataaagg
    41041 accaaccgaa ccacagttca gcccacacta acaggctaat gattagcggt aataaaaatt
    41101 gtccgctgga aacaaatgct ttaattaaaa tattggctgt cccaggtgag cgtggaaaag
    41161 cttccatcaa actgggataa gtgcctgcat cgagaaaact gtttgccatt cccgccagaa
    41221 agccaaaaac ataagcgata atgatgttat tggtctgcag gatgccaaaa aagaaggcca
    41281 tatagcaaca catcccgagc atgataaaag ggcggcgacc aaagcgatcg gataataatc
    41341 ctgcaaaaag caagacactt aatcgaccaa tgcccagcga tgagataact atcgagacac
    41401 ccgcggcatt agtctgccaa agtgtctcca gcgaggccat attcaggctc atcaaaatga
    41461 cgcccatacc atgcaccagg taattaaaat acaacccaag tgcggtaggg taatagggat
    41521 ttttcatagg ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat
    41581 ccattcatta ttgttgtaac aacgacaatt cctttctgtg gaaaaattag caaagccgct
    41641 ccagatcgtg tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat
    41701 tgccatcagg gtagactcgt tgaaatgcgg ttaagtcggg gaaaaaacgc atggtgttgt
    41761 tgccgatgcg gttatttatt ttctctatga tggcatgaag atgttttttt ctttgctgac
    41821 gcatagccaa cagttgctcg actacacgat caggaatggc acacttgcca ttttcccatt
    41881 gacgccagct ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca
    41941 tggcaaaaat atggcggagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg
    42001 aaataaacaa taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca
    42061 gtatcttgtg caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt
    42121 aaaaaaaagc ccatcgtggg agatgggcaa agactacaca cagcaattcg ttgtttcact
    42181 caggggattt ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg
    42241 catacggact ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt
    42301 gcgacaggta ctaccagctg aatggcggta agaaggaaaa tatggggaag agcaagcaaa
    42361 tatcaattag cggtggttaa caatcaggcg gcagagccgc ctgatgagaa attacttatt
    42421 cggtttctcg atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggcggcgg
    42481 gacttcttcc acccacgcgg caaacagacg ccaggaaacg gctaacagaa ccggaccaat
    42541 aaacagaccg atcatcccga aagcaatcaa accaccaata acgccagaga ggatcaggat
    42601 cagcggtaaa tcggcaccca tgcgaattaa cattgggcgg atgacgttat ccagcgtgcc
    42661 aaccacaccg ctccacacta acaataccgt tccccaggtg gtatcgccgg tccagtagag
    42721 ccagataatc gccggaatca gtaccggcaa cgggccaagc tggacaaggc aggagaggat
    42781 cattaacacc gttagcaacg ttgcataagg tacgccggat acggcgaggc cgataccgcc
    42841 aagcactgcc tgtactaacg ccgtcaccac cacacccagc gccaccgcgc ggatagcctg
    42901 tgccgccagc agcacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg
    42961 aatgccttgt gccacctgtt caccgcgcca gtacagcagg gcactgaaga gaagcatcag
    43021 cgcacaatgc accataaagc gcccgatatg cgccgcctgc ccaacgaacc aggtggtggt
    43081 ggtgccaata taagggcgga ctttcgccat gatcgccgtc ccccccatat ccagcaagtt
    43141 gtgccagcct gcatacagct tcgcgccaat caccggaatg gtattaagcc acgccagatc
    43201 gggtaacgtc atgtcgccgc tggaaatggc tttaattagc gggccgctgc cgtcgacgat
    43261 actgttaacc agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcatcac
    43321 cagaacggcg agagagcggc ggccaaacat gatcttttgc aaacgtaaca ataccggcca
    43381 ggtggcgata accaccgtac cggcccatgc aaagccgaga ataaagggtt gaacaatcca
    43441 cagacatgcc acaatcatga tggctaaaaa cagcaccgaa agcagaattt gtgcgacatc
    43501 cctgggctga cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg
    43561 acgcgacgtg aacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa
    43621 caggccgatt ctgcccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact
    43681 caggcaacac gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg cggctacgtt
    43741 cgttcacccc agtcacttac tattgtaagc tcctggagtt cactcgcttg ccgccttcct
    43801 gcaactcgaa atccatagag taaataaaca tcacctttca gtgcgctatt gcagccgcct
    43861 gaaagaatag tgtatcgacc tacatcacag accgcaggaa agggtcaata taatgattcc
    43921 acagatttcc caggcacccg gcgtcgttca actggtgctt aattttttgc aagagctgga
    43981 gcaacaaggt tttaccggcg atacggcgac aagttatgcc gatcgtctga caatgtcgac
    44041 cgacaacagt atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga
    44101 tgtggcgctg atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc
    44161 ccgcggcggc ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat
    44221 gtcccgccat atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga
    44281 ggccggggtg ataaaagatc aactcaatca gtacctgaaa ccgttcggct acttttttgc
    44341 gccggaactt tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcatc
    44401 cggtcaggga tcgctggtct atggcaaaac gtcggatcac gtacttggcg tacgcgcggt
    44461 gttgttgggg ggcgatattc tcgatacgca acctttaccc gtcgaactgg cggaaacgct
    44521 gggtaaatcc aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca
    44581 gcaacgccag ttaatcatcg acaacttccc caaacttaac cgctttctta ccggttacga
    44641 tctgcgtcat gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg
    44701 ttcagaaggg acgctggcct ttattaccga agcgcggctg gatattacgc ccttgcctaa
    44761 agtgcgccgt ctggtgaacg tcaaatatga ctcttttgac tccgcgctgc gtaacgcgcc
    44821 gtttatggtt gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct
    44881 ggcgcgggaa gatattgtct ggcattccgt cagcgagttg attaccgatg tgcctgacaa
    44941 agagatgctc gggctgaaca ttgtggagtt tgctggtgat gatgaggcgc tgattgacga
    45001 gcgagttaat gctctctgtg tgcggcttga tgagctgatt gccagccaac aggcaggtgt
    45061 gattggctgg caggtgtgcc gcgagctggc gggtgttgaa cgtatctatg cgatgcgtaa
    45121 aaaagccgtt ggtctgcttg gcaatgccaa aggtgccgct aagccaattc cgtttgctga
    45181 ggatacctgc gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct
    45241 cgacagccac ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt
    45301 ccgtccagcg ctggatatgt gcgatcctca acaagagatt ttgatgaagc aaatctctga
    45361 tgacgtagtg gcgctgactg cgaaatacgg tggtttgttg tggggcgagc acggcaaagg
    45421 ttttcgcgct gaatacagcc cggcgttttt cggtgaggaa ctttttgcag aactgcgcaa
    45481 agtgaaagcg gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga
    45541 aggtctcgat gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca
    45601 gatccccatt gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt
    45661 atgcttcaac tttgatgccc gtagtccgat gtgtccgtcg atgaagatca cccagaaccg
    45721 gattcattca ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgtt tgttggcgga
    45781 tcgcggcgtt gatccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg
    45841 gacgttaatt gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat atgacttctc
    45901 acacgaagtc aaagaggcga tgtcgggctg tctggcctgt aaagcgtgtt cgacccagtg
    45961 ccccatcaaa attgatgtgc cggagtttcg ctctcgtttt ctgcagctct atcacacccg
    46021 ttatttacgc ccgctgcgcg accacctcgt cgctacggtc gagagctacg cgccgctgat
    46081 ggcacgcgcg ccgaaaacct ttaacttctt cattaatcag ccgctggtgc gcaaactctc
    46141 ggaaaaacat atcggcatgg ttgatttgcc gctgctgtcg gtcccctcgc tacaacaaca
    46201 aatggtgggg catcgctcgg caaacatgac gctggaacag cttgaatctc tcaatgcaga
    46261 gcagaaagcg cgcacagtgt tggtggtgca ggaccccttt accagctatt acgatgcgca
    46321 agtggtggcg gattttgtcc gtctggtcga aaaattaggt ttccagcctg tgttactgcc
    46381 attttcgcca aatggcaaag cccagcatat taaaggtttt cttaatcgtt ttgcgaagac
    46441 ggcgaaaaag acggcggatt tcctcaaccg tatggcgaag ctgggtatgc caatggtagg
    46501 cgtcgatccg gcgctggtac tttgttatcg cgatgaatat aaactggcac tgggcgagga
    46561 gcgtggcgag tttaacgtct tactggcgaa tgaatggctg gcaagcgcac ttgagtcaca
    46621 gccagtggct acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaagttac
    46681 cgccttgccg ggtgcgccag cacaatgggc cgcgatattt gcccgttttg gcgcgaaact
    46741 ggaaaatgtc agcgtgggtt gctgcggcat ggcagggact tacggacatg aagcgaaaaa
    46801 ccatgaaaat tcgctcggga tctatgagtt atcctggcat caggctatgc agcgactgcc
    46861 gcgtaaccgc tgtctggcga ccggatattc ctgccgtagc caggtaaaac gggttgaagg
    46921 cacgggggta cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacgg
    46981 aaaatcaccc tggaagcact gaatgctatg ggtgaaggaa acatggtggg gttcctggat
    47041 attcgctttg aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg
    47101 acaaagcagc ctttcgggtt gctgcatgga ggagcatccg tggtactggc cgaaagtatc
    47161 ggttccgttg ccggttattt atgtaccgaa ggtgagcaaa aagtggttgg tctggaaatc
    47221 aatgctaacc acgtccgctc ggcacgagaa gggcgggtgc gcggggtatg caaaccgttg
    47281 catctcggtt cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg
    47341 tgctgttcgt cacgattgac gaccgccatt ttgtgacgtt gtcaccagaa aagtgtgacg
    47401 catattgcct ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct
    47461 aaaagcgagg atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta
    47521 cgccgtgacc agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa
    47581 cttgaatttg ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttt
    47641 gcctggcgtc tgggcgtgca ttgatcctga cttaatgccg caagttcgcc aaatcgacct
    47701 ggattaacct ccgtccaggt tcgatgacct tcttaatact gcttatctac cgcctgatat
    47761 tgttcaaaaa taatactaat cgcagtttgt gttaaaacgg cgggttagct ttatgagatt
    47821 attcttatcg ccccttcaag agctaagcca ctgagagtgc cggagataag cgccggatgg
    47881 ggtagaaacc cttaagcctg tgttgcacag acttaagggt tttcttattt ctgaacgctg
    47941 cactttttta cctatctctt aaatagtcat tttcaataca actttttctt tttcatcaca
    48001 gccttaatgc tccttagata ataaccatta tctaacaatg agatacctaa ttcttagcgt
    48061 gcctgttaac ccacacatca gggtctatgc ttattaaaag caggcaaaaa gcatgtttta
    48121 cggtaaagcc cctgcgtttg ctgggttgta ctgataatca ttatcactaa catgctgtta
    48181 tacgctgaaa gcgatgaagt gaggtaaatc gatggacatg cattcaggaa cctttaaccc
    48241 acaagatttc gcctggcaag gcttaacgct gacacccgca gcggcgatac acatccgtga
    48301 gctggtggca aagcagccgg gtatggtcgg cgtgcgctta ggcgtgaagc aaacgggctg
    48361 cgcgggcttt ggctatgtgc tcgacagtgt tagcgagccg gacaaagacg atctgctgtt
    48421 tgaacacgac ggcgcgaagc tgtttgtccc gctgcaagcg atgccgttta ttgatggcac
    48481 ggaagtcgat ttcgttcgtg aaggacttaa tcagatattc aaatttcaca accctaaagc
    48541 ccagaatgaa tgtggctgtg gcgaaagctt tggggtatag gcggtactat gtctcgtaat
    48601 actgaagcaa ctgacgatgt caaaacctgg accggcggcc cgctgaatta taaagaagga
    48661 ttcttcaccc agttagccac cgatgagctg gcaaagggga taaacgaaga ggtggtgcgc
    48721 gcaatttcgg cgaagcgtaa tgagccggag tggatgctga agtttcgtct aaacgcctat
    48781 cgcgcatggc tggagatgga agaaccgcac tggttgaaag cgcactacga caagctgaat
    48841 tatcaggatt acagctacta ctcagcacca tcgtgcggta attgtgacga cacttgcgcg
    48901 tctgaacctg gcgcggtgca gcaaactggc gcgaacgcct ttttaagtaa agaggtggag
    48961 gcggcgtttg agcagttggg cgttcccgtg cgggaaggca aagaggtggc ggtggatgcc
    49021 attttcgact cagtttcggt tgccactact tatcgcgaaa aactggcgga gcagggaatt
    49081 attttctgtt cctttggtga ggcgatccac gatcacccgg aactggtgcg taaatatctc
    49141 ggcaccgtgg tgccggggaa tgacaacttc tttgccgcgc ttaatgcggc ggtagcctct
    49201 gacggtacgt ttatttatgt gcctaaaggc gtgcgctgcc cgatggaact ttccacctat
    49261 tttcgcatta acgcggaaaa aaccgggcag tttgagcgca ccattctggt ggccgacgaa
    49321 gacagctacg tcagctacat tgaaggctgt tccgctccgg tgcgtgacag ctatcagtta
    49381 cacgcggcag tggtggaagt catcatccat aaaaacgccg aggtgaaata ttccacggta
    49441 caaaactggt tccctggcga taacaacacc ggcggtattc tcaacttcgt caccaagcgt
    49501 gctttgtgcg aaggcgaaaa cagcaaaatg tcatggacgc aatcagaaac cgggtccgcg
    49561 attacgtgga aatatcccag ctgcattttg cgcggcgata actccattgg tgagttttac
    49621 tcagtggcgc tgaccagcgg tcatcagcaa gcggataccg gcaccaagat gatccacatc
    49681 ggtaaaaaca ccaaatcgac cattatctcg aaagggatct ctgccggaca tagtcagaac
    49741 agttatcgcg gcttagtgaa aatcatgccg acggcaacca atgcgcgcaa tttcactcag
    49801 tgcgactcaa tgctgattgg cgctaattgt ggggcgcata ccttcccgta tgtcgaatgt
    49861 cgcaataaca gcgcacaact ggagcacgag gcaacgacat cacgtattgg tgaagatcaa
    49921 ctgttttact gcctgcaacg cgggatcagc gaagaagacg ccatctcgat gattgttaac
    49981 ggtttctgca aagacgtgtt ctcagaactg ccgctggaat ttgccgttga agcacaaaaa
    50041 ctcctcgcca tcagtcttga acacagcgtc ggataaggaa taaacatgtt aagtattaaa
    50101 gatttacacg tcagcgtgga agataaagct atcctgcgtg gattaagcct cgccgttcgt
    50161 cccggcgaag ttcacgccat tatggggcca aacggttcgg gcaaaagtac cttatcggca
    50221 acgcttgccg ggcgcgaaga ttatgaagtg acgggcggca cggttgagtt caaaggcaaa
    50281 gatttgcttg cactgtcgcc ggaagatcgt gcaggcgaag gcatctttat ggccttccag
    50341 tatccggtgg agattccagg cgtcagtaac cagtttttcc tgcaaacggc acttaatgca
    50401 gtgcgcagct atcgcggcca ggaaacgctc gaccgctttg attttcagga tttgatggaa
    50461 gagaaaatcg ctctcctgaa gatgccggaa gatttattaa cccgttcggt aaacgttggt
    50521 ttttccggcg gcgagaaaaa gcgcaacgat attttgcaaa tggcggtgct ggaaccggag
    50581 ttatgcattc ttgatgagtc ggactccggg ctggatattg acgcattaaa agtggtcgcc
    50641 gatggcgtga actcgctgcg tgatggcaag cgctcattca tcattgttac gcactaccaa
    50701 cgcattctcg actacatcaa gcctgattac gtccatgtgc tatatcaggg acgaattgtg
    50761 aaatccggcg atttcacgtt ggtcaaacaa ctggaggagc agggttatgg ctggcttacc
    50821 gaacagcagt aacgcgctgc aacagtggca tcacttgttt gaagctgaag ggacaaaacg
    50881 ctcaccgcaa gcacagcagc atttacaaca attgctgcgt accggactgc cgacacgtaa
    50941 acatgaaaac tggaaatata cgccgctgga agggctgacc aatagccagt ttgtcagcat
    51001 tgcgggagag atatccccac agcagcgaga tgccttagcg ttaacgttag acgctgtgcg
    51061 gctggtattt gtcgatggac gttacgtgtc agcactgagc gatgcgactg aaggcagcgg
    51121 gtatgaagtg agcattaacg acgaccgtca gggggtaccc gacgctattc aggcggaagt
    51181 gtttctgcat ttgacggaaa gcctggcaca aagcgtgacg catatcgccg tgaagcgcgg
    51241 tcaacggccg gcaaagccat tgctgttaat gcatattacc cagggcgtgg caggtgaaga
    51301 ggtcaacact gcccattacc gacatcatct ggatctggcg gaaggtgccg aagcaacggt
    51361 gatcgaacat tttgtcagcc ttaatgatgc tcgtcacttc accggcgcac ggttcactat
    51421 caatgtcgca gcgaacgccc acttgcagca tatcaagctg gcgtttgaaa acccggtcag
    51481 tcaccacttt gcccataacg atttgttgct ggctgacgat gccaccgcat ttagccatag
    51541 tttcctgctg ggtggcgcag tgttacgaca caacaccagt acgcaactca atggcgaaaa
    51601 cagcacgctg cggatcaata gcctggcgat gccggtgaaa aacgaggtgt gtgatacccg
    51661 tacctggctg gaacacaata aaggtttttg taacagccga cagttgcaca aaactatcgt
    51721 cagcgacaaa ggccgcgcgg tatttaacgg tttgatcaac gtcgcgcagc acgccatcaa
    51781 aacggatggt cagatgacca acaataatct gctgatgggc aaactggcag aagtggatac
    51841 gaaaccgcag ctggaaatct atgcagatga tgtgaaatgc agccacggcg cgacggtggg
    51901 gcgtattgat gatgaacaga tgttctatct gcgctcgcgc gggatcaatc agcaggatgc
    51961 ccagcagatg atcatttacg cctttgctgc cgaactgacg gaagcactgc gtgatgaggg
    52021 gcttaaacag caggtgctgg cccgaatcgg tcaacggctg ccaggaggtg caagatgact
    52081 ttttccgtcg acaaagtgcg ggccgacttt ccggtgcttt ctcgtgaggt aaacggtttg
    52141 ccgctggctt atctcgacag cgccgccagt gcgcagaaac cgagccaggt gattgacgcc
    52201 gaggccgagt tttatcgtca tggctacgcg gcggtgcatc gcggtattca taccttaagc
    52261 gcccaagcga ccgagaaaat ggagaacgtg cgcaagcggg catcgctgtt tattaatgcc
    52321 cgttcggcgg aagagctggt gttcgtccgc ggcacgacgg aagggatcaa tctggtcgcc
    52381 aatagctggg gtaacagcaa tgtgcgggcg ggcgataaca tcatcatcag tcagatggag
    52441 caccacgcta acattgttcc ctggcagatg ctttgcgcac gcgttggcgc agagctgcgt
    52501 gtgatcccgc tcaatcccga cggtacgtta caactggaga cgctgcctac gctgtttgat
    52561 gagaaaactc gcctgctggc aattactcat gtctccaacg tgcttggcac agaaaatcca
    52621 ctggcggaaa tgatcacgct tgcgcaccag catggcgcaa aagtgctggt ggatggcgct
    52681 caggcggtga tgcatcatcc ggtggatgtt caggcgctgg attgcgactt ttacgtgttc
    52741 tccgggcata aactgtatgg ccccaccgga attggcattc tttatgtgaa agaagccttg
    52801 ttgcaggaga tgccgccgtg ggaagggggc ggttctatga tcgccaccgt cagcctgagt
    52861 gaaggcacaa cctggaccaa agcaccttgg cggtttgaag ccggtacacc caataccggg
    52921 ggcatcattg gtcttggcgc ggcgctggag tatgtttcgg cgctggggct taataacata
    52981 gccgagtatg aacagaatct gatgcactac gcgctatcac agctggaatc tgtaccggat
    53041 ctcactctct atggtccgca gaacaggctt ggcgttattg cttttaatct tggtaaacac
    53101 cacgcctatg atgttggcag ttttctcgat aactacggca ttgctgtgcg taccggacat
    53161 cactgcgcta tgccattaat ggcctattac aacgtccctg ctatgtgtcg ggcgtcgctg
    53221 gccatgtata acacccatga agaagtggat cgtctggtga ccggactgca acgtattcac
    53281 cgtctgctgg gataacaggg aggcactatg gctttattgc cggataaaga aaaattgctg
    53341 cgtaattttt tacgctgcgc caattgggaa gagaaatatc tctacattat tgagctgggc
    53401 cagcgtctgc cagaattacg cgacgaagac agaagcccac aaaatagcat tcagggctgc
    53461 cagagtcagg tgtggattgt catgcgccag aatgcgcagg gaattattga attgcagggc
    53521 gacagcgatg cggcgattgt gaaagggctt attgcggtcg tctttattct ctacgatcag
    53581 atgacgccgc aggatattgt caatttcgat gtgcgtccgt ggtttgaaaa aatggcgctc
    53641 acccaacatc tcaccccatc tcgttcacaa ggtctggaag cgatgattcg cgcaattcgc
    53701 gccaaagccg ctgcacttag ctaaactaaa gggacagctt tcatccggtt ggctcacatt
    53761 gccggatggt caatgtttac actgacctgc acaggagtgt ttcggtttca ggtgaacata
    53821 agcgccatcc gacgtttcag cgtgagtctc cggcaaatac aggaggttta caatgaaacg
    53881 cgcgtctttg cttacactca cgcttatcgg cgcttttagc gccatccagg ctgcctgggc
    53941 ggttgattat ccgctaccac caaccggaag ccgactggtt gggcaaaatc aaacgtatac
    54001 ggtgcaggaa ggggataaaa accttcaggc catcgcccga cgttttgata ctgcggcaat
    54061 gttgatcctt gaagccaata acactatcgc cccggtgcca aaacccggta cgacgataac
    54121 tattccttcg caactgttat tacctgatgc gccgcgtcag gggattatcg ttaaccttgc
    54181 agagctgcgc ctttattatt atccgccggg agaaaatatc gtgcaggttt atccgatagg
    54241 tattggcttg caggggctgg aaacgccggt gatggaaacg cgtgtggggc agaaaatccc
    54301 taacccaacc tggacgccta ctgcaggcat tcgtcaacgt tcgctggagc gtggtattaa
    54361 attaccgcca gtcgttcctg ccggaccaaa taacccgcta ggacgttacg cactgcgcct
    54421 cgcgcatggt aatggcgaat acctcattca tggcaccagt gcgccggaca gcgtcggttt
    54481 gcgcgtcagt tcagggtgta ttcgcatgaa tgcaccggat attaaagcct tgttctccag
    54541 cgtgcggacg ggaacgccgg tgaaagtgat caacgaacct gtgaaatatt ccgtggagcc
    54601 taacgggatg cgttatgttg aagtacatcg accactatcg gcagaagaac agcagaacgt
    54661 tcagacaatg ccatacacac tgccagcagg ctttacgcaa tttaaagaca ataaggctgt
    54721 agatcagaag ttagtcgata aagcgttgta tcgtcgggca gggtatccgg tttcggtgag
    54781 cagtggagca actcccgcag ccagcaatgc gccttcagta gagtcagcgc agaatggtga
    54841 accagagcaa gggaatatgt tacgcgcgac gcagtagcgg taaacggcag acaaaaaaaa
    54901 tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag
    54961 ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg
    55021 aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt
    55081 cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca
    55141 ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt
    55201 taatctccat gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt
    55261 gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag
    55321 ataaaaaaat tgagagaaaa caggatgctt ccatcggatt catcttagat aaagtgagat
    55381 tatatagtga aatccgattt gattttttat tgcttctggt tatcgattaa ataaaaaaag
    55441 cgcccatcag ggcgcttcga tatacaaatt aattcacaaa agcaatatta caggacgtga
    55501 acagatgcgg tgttagtagt gccgctcggt accagtgcac cagaaaccat aactacaacg
    55561 tcacctttgt gtgccagacc gctctgcaga gccagttctt tacccagacg gtagaaatca
    55621 tcagtagaag tgatctcttt aaccagctgc ggcacaacgc ctttgctcag taccaactga
    55681 tgagccgttt tttcgttggt ggtcagtgcc aggatggtgg catccgggaa gtatttacgt
    55741 actgcgcgag cagatttacc gccctgagta gcaaccacga tcagcggagc atccagtttt
    55801 tcagcagttt caacggcacc acggcatacc gcttcggtaa tgcgcagttt acggttgtca
    55861 ttgttgaact cgagacggct gttcatcacg cggtcggtac gttcgcaaat ggtcgccatg
    55921 atagaaaccg cttccagcgg gtatttacct tttgcggatt caccagacag catcactgca
    55981 tcagtaccgt cgaggatggc gtttgcaacg tcaccggctt ctgcgcgagt cgggcgtggg
    56041 tttttgatca tggaatccag catctgggtc gcagtgataa cgattttacg tgcacggata
    56101 catttttcga tcatcatctt ctgggcgaag ataacttctt ctaccgggat ttctacaccc
    56161 aggtcgccac gcgcaaccat gatgccgtca gaggcttcga ggatttcgtc gaagttgttg
    56221 aggccttcct ggttttcgat tttggagatg atgtggatgt tttcgccgcc gtgcgctttc
    56281 aggtgctcac ggatttcgat aacgtcagaa cgcttacgaa taaaggaagc agcaacaaag
    56341 tctacgcctt gttcgcaacc aaagatcagg tcctgtttgt ctttttcagc cagtgctggc
    56401 agagcaatgg aaacgccagg caggttcaca cctttgtttt cgcccaggtc accgttgttc
    56461 agcactttac agataacttt gttaccttca atggcggtaa cttccatacc gatcagacca
    56521 tcgtcaacca gtacggtgtt gccaactgac aggtcagtag tgaaaccttc atacgttacc
    56581 gcaaccattt cgctgttgcc gataacagat ttatcagtgg tgaaagtaaa ggtctgacca
    56641 gctttcagag aaacgtcgtt accgccttcc agtttcatgg tgcggatttc cggacctttg
    56701 gtatcaagca ggatagcggc ggttttacca gttttgctca tcacgttgcg cagattctga
    56761 atgcgctgac cgtgttctgc atagtcacca tgagagaagt tcagacgcat aacgttcatg
    56821 ccagcgtcca gcattttagc taacatctct tcagattcgg ttttcggtcc gatggtgcaa
    56881 acaattttgg tctttttcat gacagtctta gtctttaagt tgagaaggat gggagaaact
    56941 tgctttctgg gcgcactgcc ggaaagcgaa tccggtatta cgaaagtggt ggtgcctcgc
    57001 tctaaggata ggtgacatcg aaaaagcgtg cagaggaatg tgtgcttgtg tctcagccca
    57061 acgtgagtgc ctgtttttga gtcgttgagt tctacagtcc aaagtgctga aaccattcaa
    57121 gagtcaattg gcgcgcatta tacgctgaaa ttaatccaaa aggaaaacaa aaacagcgtt
    57181 tcaaaaaata tacaacttgt tagacaataa attgttatca aagatttaac gttccaggga
    57241 aaaggttacg caaaaacttc actgtttgcg caacgtctgg tgccgaagtt gtcagtgtga
    57301 ggataagtga aagttcatcc ggtgtataaa tacatcatcg ctcaattgct acagttaaaa
    57361 taatttatcg cgttatttgt ctgattttgt ttgatttttg tttaattggt tagattgata
    57421 tatgctgata gttggcatga taaataggta taaagaatgg gaaatcggac aaaagaggat
    57481 gaactgtacc gagaaatgtg cagagttgtc ggtaaagttg tgctggaaat gcgcgatctg
    57541 gggcaggaac caaagcatat tgttatcgca ggcgtattac gtacagcatt agccaataaa
    57601 cgcattcagc gcagtgagct ggaaaagcag gcgatggaaa ccgtcattaa cgcactggta
    57661 aaatgaccat tcaggatgag gcggtaagct tcatcctgat attcttgctt cagttgcggt
    57721 ccagttaatc attctaaata acaactgctg
//
LOCUS       DAZGSP010000020        55591 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZGSP010000020 DAZGSP010000000
VERSION     DAZGSP010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.020"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            301..1665
                     /gene="yajR"
                     /locus_tag="ACHK74_001354"
     CDS             301..1665
                     /gene="yajR"
                     /locus_tag="ACHK74_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0220209.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRVEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1793..2284)
                     /gene="yajQ"
                     /locus_tag="ACHK74_001355"
     CDS             complement(1793..2284)
                     /gene="yajQ"
                     /locus_tag="ACHK74_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFP0220210.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2452..3360
                     /gene="panE"
                     /locus_tag="ACHK74_001356"
     CDS             2452..3360
                     /gene="panE"
                     /locus_tag="ACHK74_001356"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP0220211.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRP"
     gene            3326..3916
                     /gene="yajL"
                     /locus_tag="ACHK74_001357"
     CDS             3326..3916
                     /gene="yajL"
                     /locus_tag="ACHK74_001357"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFP0220212.1"
                     /translation="MSASALVCLAPSSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3970..5418)
                     /gene="thiI"
                     /locus_tag="ACHK74_001358"
     CDS             complement(3970..5418)
                     /gene="thiI"
                     /locus_tag="ACHK74_001358"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFP0220213.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNDFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQSK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5624..5866
                     /gene="xseB"
                     /locus_tag="ACHK74_001359"
     CDS             5624..5866
                     /gene="xseB"
                     /locus_tag="ACHK74_001359"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFP0220214.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5866..6765
                     /gene="ispA"
                     /locus_tag="ACHK74_001360"
     CDS             5866..6765
                     /gene="ispA"
                     /locus_tag="ACHK74_001360"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFP0220215.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6790..8652
                     /gene="dxs"
                     /locus_tag="ACHK74_001361"
     CDS             6790..8652
                     /gene="dxs"
                     /locus_tag="ACHK74_001361"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFP0220216.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGFKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8707..9681
                     /gene="yajO"
                     /locus_tag="ACHK74_001362"
     CDS             8707..9681
                     /gene="yajO"
                     /locus_tag="ACHK74_001362"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFP0220217.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9735..10253)
                     /gene="pgpA"
                     /locus_tag="ACHK74_001363"
     CDS             complement(9735..10253)
                     /gene="pgpA"
                     /locus_tag="ACHK74_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFP0220218.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10231..11208)
                     /gene="thiL"
                     /locus_tag="ACHK74_001364"
     CDS             complement(10231..11208)
                     /gene="thiL"
                     /locus_tag="ACHK74_001364"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFP0220219.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLGLLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11286..11705)
                     /gene="nusB"
                     /locus_tag="ACHK74_001365"
     CDS             complement(11286..11705)
                     /gene="nusB"
                     /locus_tag="ACHK74_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFP0220220.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11725..12195)
                     /gene="ribE"
                     /locus_tag="ACHK74_001366"
     CDS             complement(11725..12195)
                     /gene="ribE"
                     /locus_tag="ACHK74_001366"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFP0220221.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12284..13387)
                     /gene="ribD"
                     /locus_tag="ACHK74_001367"
     CDS             complement(12284..13387)
                     /gene="ribD"
                     /locus_tag="ACHK74_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFP0220222.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQSGIDVSHGLMMSEVEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQSFRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQVGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13391..13840)
                     /gene="nrdR"
                     /locus_tag="ACHK74_001368"
     CDS             complement(13391..13840)
                     /gene="nrdR"
                     /locus_tag="ACHK74_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFP0220223.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13991..14530
                     /gene="yajI"
                     /locus_tag="ACHK74_001369"
     CDS             13991..14530
                     /gene="yajI"
                     /locus_tag="ACHK74_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP0220224.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESRIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     IPVEQLDFVRIHDIQPVMQ"
     gene            14829..15713
                     /gene="tsx"
                     /locus_tag="ACHK74_001370"
     CDS             14829..15713
                     /gene="tsx"
                     /locus_tag="ACHK74_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFP0220225.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15890..16237)
                     /gene="yajD"
                     /locus_tag="ACHK74_001371"
     CDS             complement(15890..16237)
                     /gene="yajD"
                     /locus_tag="ACHK74_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFP0220226.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16366..17337)
                     /gene="secF"
                     /locus_tag="ACHK74_001372"
     CDS             complement(16366..17337)
                     /gene="secF"
                     /locus_tag="ACHK74_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFP0220227.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17348..19195)
                     /gene="secD"
                     /locus_tag="ACHK74_001373"
     CDS             complement(17348..19195)
                     /gene="secD"
                     /locus_tag="ACHK74_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFP0220228.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19223..19555)
                     /gene="yajC"
                     /locus_tag="ACHK74_001374"
     CDS             complement(19223..19555)
                     /gene="yajC"
                     /locus_tag="ACHK74_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFP0220229.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19578..20705)
                     /gene="tgt"
                     /locus_tag="ACHK74_001375"
     CDS             complement(19578..20705)
                     /gene="tgt"
                     /locus_tag="ACHK74_001375"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFP0220230.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20761..21831)
                     /gene="queA"
                     /locus_tag="ACHK74_001376"
     CDS             complement(20761..21831)
                     /gene="queA"
                     /locus_tag="ACHK74_001376"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFP0220231.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21924..22505
                     /gene="acpH"
                     /locus_tag="ACHK74_001377"
     CDS             21924..22505
                     /gene="acpH"
                     /locus_tag="ACHK74_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFP0220232.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22510..24327)
                     /gene="malZ"
                     /locus_tag="ACHK74_001378"
     CDS             complement(22510..24327)
                     /gene="malZ"
                     /locus_tag="ACHK74_001378"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFP0220233.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(24483..25856)
                     /gene="proY"
                     /locus_tag="ACHK74_001379"
     CDS             complement(24483..25856)
                     /gene="proY"
                     /locus_tag="ACHK74_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFP0220234.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25932..27251)
                     /gene="brnQ"
                     /locus_tag="ACHK74_001380"
     CDS             complement(25932..27251)
                     /gene="brnQ"
                     /locus_tag="ACHK74_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFP0220235.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27658..28953)
                     /gene="phoR"
                     /locus_tag="ACHK74_001381"
     CDS             complement(27658..28953)
                     /gene="phoR"
                     /locus_tag="ACHK74_001381"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFP0220236.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDSGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAAEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(29011..29700)
                     /gene="phoB"
                     /locus_tag="ACHK74_001382"
     CDS             complement(29011..29700)
                     /gene="phoB"
                     /locus_tag="ACHK74_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFP0220237.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29890..31092
                     /gene="sbcD"
                     /locus_tag="ACHK74_001383"
     CDS             29890..31092
                     /gene="sbcD"
                     /locus_tag="ACHK74_001383"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFP0220238.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            31089..34232
                     /gene="sbcC"
                     /locus_tag="ACHK74_001384"
     CDS             31089..34232
                     /gene="sbcC"
                     /locus_tag="ACHK74_001384"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFP0220239.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     ANQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNTLAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34358..35542
                     /gene="araJ"
                     /locus_tag="ACHK74_001385"
     CDS             34358..35542
                     /gene="araJ"
                     /locus_tag="ACHK74_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFP0220240.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35685..36592)
                     /gene="mak"
                     /locus_tag="ACHK74_001386"
                     /pseudo
     CDS             complement(35685..36592)
                     /gene="mak"
                     /locus_tag="ACHK74_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
     gene            36717..37628
                     /gene="rdgC"
                     /locus_tag="ACHK74_001387"
     CDS             36717..37628
                     /gene="rdgC"
                     /locus_tag="ACHK74_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFP0220241.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37706..38125)
                     /locus_tag="ACHK74_001388"
     CDS             complement(37706..38125)
                     /locus_tag="ACHK74_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220242.1"
                     /translation="MYDLLTPTINLQIHCTRYPMRSQNGGSTDLPRYWITLDKNVIWD
                     YPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAELLTKQFTSDKWGL
                     VNILRAADRRIGMRRLDQLQHKTHNIAALKIIARRSE"
     gene            complement(38329..38613)
                     /gene="ppnP"
                     /locus_tag="ACHK74_001389"
     CDS             complement(38329..38613)
                     /gene="ppnP"
                     /locus_tag="ACHK74_001389"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFP0220243.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(38685..39362)
                     /gene="aroM"
                     /locus_tag="ACHK74_001390"
     CDS             complement(38685..39362)
                     /gene="aroM"
                     /locus_tag="ACHK74_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFP0220244.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(39620..39811)
                     /gene="yaiA"
                     /locus_tag="ACHK74_001391"
     CDS             complement(39620..39811)
                     /gene="yaiA"
                     /locus_tag="ACHK74_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFP0220245.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(39861..40385)
                     /gene="aroL"
                     /locus_tag="ACHK74_001392"
     CDS             complement(39861..40385)
                     /gene="aroL"
                     /locus_tag="ACHK74_001392"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFP0220246.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(40568..41026)
                     /gene="yaiI"
                     /locus_tag="ACHK74_001393"
     CDS             complement(40568..41026)
                     /gene="yaiI"
                     /locus_tag="ACHK74_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFP0220247.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            41146..41955
                     /gene="proC"
                     /locus_tag="ACHK74_001394"
     CDS             41146..41955
                     /gene="proC"
                     /locus_tag="ACHK74_001394"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFP0220248.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(41972..43087)
                     /gene="adrA"
                     /locus_tag="ACHK74_001395"
                     /gene_synonym="dgcC"
     CDS             complement(41972..43087)
                     /gene="adrA"
                     /locus_tag="ACHK74_001395"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFP0220249.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSWEIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(43189..43509)
                     /gene="psiF"
                     /locus_tag="ACHK74_001396"
     CDS             complement(43189..43509)
                     /gene="psiF"
                     /locus_tag="ACHK74_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFP0220250.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRITSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(43628..45043)
                     /gene="phoA"
                     /locus_tag="ACHK74_001397"
     CDS             complement(43628..45043)
                     /gene="phoA"
                     /locus_tag="ACHK74_001397"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFP0220251.1"
                     /translation="MKQSTIALALLSLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(45144..45404)
                     /gene="iraP"
                     /locus_tag="ACHK74_001398"
     CDS             complement(45144..45404)
                     /gene="iraP"
                     /locus_tag="ACHK74_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFP0220252.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(45586..45789)
                     /locus_tag="ACHK74_001399"
     CDS             complement(45586..45789)
                     /locus_tag="ACHK74_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220253.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYFWLKWPCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            45867..46961
                     /gene="ddlA"
                     /locus_tag="ACHK74_001400"
     CDS             45867..46961
                     /gene="ddlA"
                     /locus_tag="ACHK74_001400"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP0220254.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(46985..47197)
                     /gene="yaiZ"
                     /locus_tag="ACHK74_001401"
     CDS             complement(46985..47197)
                     /gene="yaiZ"
                     /locus_tag="ACHK74_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFP0220255.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            47457..47765
                     /gene="yaiY"
                     /locus_tag="ACHK74_001402"
     CDS             47457..47765
                     /gene="yaiY"
                     /locus_tag="ACHK74_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFP0220256.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(47824..48918)
                     /gene="yaiW"
                     /locus_tag="ACHK74_001403"
     CDS             complement(47824..48918)
                     /gene="yaiW"
                     /locus_tag="ACHK74_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFP0220257.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(48931..50139)
                     /gene="sbmA"
                     /locus_tag="ACHK74_001404"
                     /pseudo
     CDS             complement(48931..50139)
                     /gene="sbmA"
                     /locus_tag="ACHK74_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
     gene            50502..51659
                     /gene="ampH"
                     /locus_tag="ACHK74_001405"
     CDS             50502..51659
                     /gene="ampH"
                     /locus_tag="ACHK74_001405"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFP0220258.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(51660..52283)
                     /gene="iprA"
                     /locus_tag="ACHK74_001406"
     CDS             complement(51660..52283)
                     /gene="iprA"
                     /locus_tag="ACHK74_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFP0220259.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(52371..55349)
                     /gene="ehaB"
                     /locus_tag="ACHK74_001407"
     CDS             complement(52371..55349)
                     /gene="ehaB"
                     /locus_tag="ACHK74_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFP0220260.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCAERANDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVDVYNIGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWSDEGTSGWFGNTGNASDYNGGDWNRDDIALAVIAHPAAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPHGRDSYRDADGDVDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHLTVDTNDDGVPDAYGPVYNDNGVVIDTSTGTEATATLIDIAANS
                     LWPSSTVGVENSDSEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINS
                     GSTVNVADSTLISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGD
                     AALYANNIKVTNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLD
                     ADLTNDRTNDTTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVA
                     AATGNYKVRIDNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRG
                     NTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFG
                     GNFNGDNGTINYDQDVNGIMVGVDTKIDDNNAKWIVGAAAGFAKGDMNDRSGQVDQDS
                     QTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYD
                     FKLGDAGYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSED
                     QALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANY
                     LGGGDVDQDWSANVGVKYTW"
BASE COUNT        13881 a        14662 c        13802 g        13246 t
ORIGIN      
        1 tttaacctga aacattatca tcgatgtcac caaatcgttt atttagtgag ataaaaatag
       61 catgttcgta agataaaaaa tgcacatagc aatcaagaaa taatattatt acttttgatg
      121 taaaactatt tagctttaag attcattttt ttcaacatat agagcattca ttttatgaat
      181 gcaaaataaa catacctgac acacaaacta ttctcctctg taatattttc tcattaacac
      241 tctgtttacc caacatgcgc tccgcactac actatgtggg cattttttta aggggtggta
      301 atgaacgatt ataaaatgac gccaggtgag aggcgcgcga cctggggttt agggaccgta
      361 ttctcgttgc gcatgctggg catgtttatg gttctgccgg ttctgaccac gtacggcatg
      421 gctctgcaag gtgccagcga agcattaatc ggtattgcca ttggtattta tggtctgact
      481 caggccgttt ttcagattcc gtttggcctg ctttccgacc gcattggtcg caaaccatta
      541 attgtcggtg ggctggcggt gtttgccgcc ggtagcgtta tcgctgcgct ttccgactct
      601 atctggggaa ttattctggg ccgggcgtta caaggctccg gtgcgattgc cgctgccgtt
      661 atggcgctgc tttccgatct cacgcgcgaa caaaaccgca ccaaagcaat ggcgtttatc
      721 ggcgtgagct ttggcattac ctttgccatt gcgatggtgc ttggcccgat catcactcac
      781 aaacttgggc tgcacgcgct gttctggatg atcgctattc tggcaacgac cggcattgca
      841 ttgaccattt gggttgtgcc caacagtagc actcacgtac ttaatcgtga gtccggaatg
      901 gtgaaaggca gtttcagtaa agtgctggcg gaaccgcggc tactgaaact caactttggc
      961 attatgtgtc tgcatatttt gctgatgtcg acgtttgttg ccctgcccgg acagctggct
     1021 gatgcggggt tcccggcggc tgaacactgg aaggtctacc tggcaacgat gctaatcgcc
     1081 tttggctcgg tcgtgccttt cattatctac gctgaagtta agcgcaaaat gaagcaagtc
     1141 tttgtcttct gcgtcgggtt gatcgtagtt gcggaaattg tgttgtggaa cgcacaaacg
     1201 cagttctggc aacttgtggt cggcgtgcag cttttctttg tggcgtttaa tttgatggaa
     1261 gcccttctgc cctcacttat cagtaaagag tcgccagcag gttacaaagg tacggcgatg
     1321 ggtgtctact ccaccagcca gtttcttggc gtggcgattg gcggttcgct gggcggctgg
     1381 attgacggca tgtttgacgg tcagggggta tttctcgctg gcgcaatgct ggccgcagtg
     1441 tggctggcag tcgccagtac catgaaagaa ccgccgtatg tcagcagctt gcgcgttgaa
     1501 atcccagcgg acattgccgc aaacgaagcg ttaaaagtac gtttgctgga aactgaaggc
     1561 gtcaaagaag tgttgattgc agaagaagaa cattcagctt atgtgaaaat cgacagcaaa
     1621 gtgacgaatc gctttgaggt tgaacaggca attcgtcagg cataaaaaac gaggcgaatt
     1681 gcctgctgcg cttagcttac caggcctaca aagtttctga attacaaacc tttgtaggcc
     1741 gaatcaggcg ttcatcccgc atctgacatg aacaaaacgc acatagtcgc gattaatcgc
     1801 ggaagttttt gaactggaac ggctgaccga gatcgccacc gcgtaccatc gccatgacag
     1861 cctgcaaatc atcacgagat ttgcccgtta cgcggatttc atcgccctga atttgcgcct
     1921 gcactttcag tttgctgtct ttgatcatct tgacgatttt cttctgggtc gcgctctcaa
     1981 tgccctgttt cagtttcgct tccacaaacc aggttttacc gctatgaacg atattctccg
     2041 gtacatccag cgaactgcct tcaatgccgc gcttcagcag cttggcacgc agaatatcca
     2101 gcaactgatt gacctggaaa tcggactcgc tcaacacttt gatggttttg ctggcgtcgt
     2161 tcagctcaaa tgaggcttca acattacgga agtcaaaacg ggactccact tcgcggctcg
     2221 cgttatcgac cgcgttacgt gcttcctgaa gatcaacttc agagacaata tcgaaagatg
     2281 gcatcttttc ctctcccttc atttttgatg cgaagcataa tacccgcaaa gttaaataac
     2341 cgacagtcct gcaccaggac gtatccctta attgtgcata gtcgcaacag catgcacaat
     2401 taagggatag ccttattgaa cacccggagt ggttgcgggt gaggaggaac aatgaaaatt
     2461 accgtattgg gatgcggtgc cttagggcaa ttatggctta cagcactttg taaacagggt
     2521 cacgacgttc agggctggct gcgcgtaccg caaccttatt gtagtgtgaa tctggttgag
     2581 acagatggtt cgatatttaa cgaatcgctg accgctaacg atcccgattt tctcgccacc
     2641 agcgatctgc tcctggtgac gctgaaagca tggcaggttt ccgatgccgt caaaagcctc
     2701 gcgtccacac tgcctgtaac cacgccaata ctgttaattc acaacggcat gggcaccatc
     2761 gaagagttgc aaaacattca gcagccatta ctgatgggca ccaccaccca tgccgcccgc
     2821 cgcgacggca atgtcattat tcatgtggca aacggtatca cgcatattgg cccggcacgg
     2881 caacaggacg gcgattacag ttatctggcg gatattttgc aaaccgtgtt gcctgacgtc
     2941 gcgtggcata acaatattcg agccgagttg tggcgcaagc tggcagtcaa ctgcgtgatt
     3001 aatccactga ccgccatctg gaattgcccg aacggtgaat tacgtcatca tccgcaagaa
     3061 attatgcaaa tatgcgaaga agtcgcggca gtgatcgaac gcgaagggca tcatacttca
     3121 gcggaagatt tgcgtgatta cgtgatgcag gtgattgatg caacagcgga aaatatctcg
     3181 tcgatgttgc aggatatccg cgcgctgcgc cacaccgaaa tcgactatat caatggtttt
     3241 ctcttacgcc gcgcccgcgc gcatgggatt gccgtaccgg aaaacacccg cctgtttgaa
     3301 atggtaaaaa gaaaggagag tgaatatgag cgcatcggca ctggtttgcc tcgcccctag
     3361 tagtgaagag actgaagccg tcaccacgat cgacctcttg gttcgcggcg gtatcaaagt
     3421 caccactgcc agcgtagcca gcgatggcaa cctggcaatt acctgctcgc gcggcgtgaa
     3481 gctgctggcg gatgcgccgc tggtcgaagt ggctgatggc gaatatgacg tgatcgtgct
     3541 gcctggtggc attaaaggcg cggagtgttt tcgcgatagc accctgctgg ttgaaaccgt
     3601 taaacagttc caccgttccg ggcgtatcgt cgcggctatt tgcgccgcgc cagccaccgt
     3661 gctggtgccg cacgatatct tcccgattgg taatatgacc ggcttcccga cgctgaaaga
     3721 caaaattccc gccgaacaat ggcaggacaa gcgcgtcgtc tgggatgcac gggtaaaatt
     3781 gctgaccagc caggggccgg gtacggctat tgattttggt ctgaaaatta tcgacctgct
     3841 ggttgggcgc gagaaagccc atgaagtggc atcacaactg gtgatggcgg cagggattta
     3901 taattattac gagtagtgtc ggatgcggca aacgtcgcat ctgaccagat gcgacgtaaa
     3961 aaccacaaat tacgggcgat acaccttcac attattaaag ccctgctcgc gcagatagag
     4021 cgcctgcagg cggctcatca ccccacgctc acaccacaat agccaggttt tgctctggtc
     4081 gagatcgcca aatttggtgc tcagtttata gaacggcaga gaaaccacat caatcccttc
     4141 gactttcagt ggcttatctt cctgttcatc gacagaacgg atatcgagaa tcacgtcgtt
     4201 cgggccgaag tcattgacgg tttccacttc caccacttcc tgctcggtct gctgggcgat
     4261 ttcgcggata tcaacgttat tcgcttcttc aaccacttta tcgagaatgc tgaagtcgaa
     4321 cttctcttct tccgcttcaa tcttcgattt aaccgctttc accgtcgggc ttttggagat
     4381 cacgccacaa tattccggca tcgtgcgagc aaagtcttcg gtgccaatct ggcgagccag
     4441 gttgatgatg tgctctttgt cgtaagagat cagcggacgc aggatcaacg tatcggagac
     4501 attatcaatt aaacgcagat tggtcagggt ctggctggac acctggccca gtgcttcgcc
     4561 ggtgaccagc gcctgtacgc cgtaacgttc agccacttta gacgcggcac gcaccatcat
     4621 acgtttgagg ataacgccca tctgaccgtc gtcgattttc tcgagaattt ccccgacgac
     4681 cggctcgaaa ttaatagcga caaaacgcac gcggtgggag ctgccaaagc ggttccacag
     4741 ataatgtgcc acctgacgca cgccaatttc atgcgccgcg ccgccaaggt taaagaagca
     4801 gtaatgcacg cggcagccgc gacgcatcaa catataactg gaaacaccgg agtcgaaacc
     4861 accggaaatg agcgacagca catcttcctg ggtgccgatc gggaaaccgc caataccttc
     4921 gtagcggcct ttaatcagca ggagacgatc gtcttccact tccagatgga cagtcacttc
     4981 cggattggtc agcttcacgc gcgcggattc aatatgctga tttaaaccgc cgccgacgta
     5041 acgttccaca tcaatcgagc taaaatcatg tttgccacgg cgcttcacgc gtacgcagaa
     5101 ggttttgcct tccagctgat cgcgatactg aaccaacgct ttctcgaaaa tatcgtgcat
     5161 gtcggtaaac ggcacgtctt cgacttcgag aatatggtgg atacccggaa tacgggtcag
     5221 agcgtcgcga atagccagac gctggttttc atctttcgcg cgaacttcga tgttatccca
     5281 gtggcggacg acagcgagcg tctcatcata gtgctttaaa acgttacgaa tgttcccggt
     5341 aaggattttt ataaagcgca agcgcacaga ttggcttttg atggtgattt ccgggaacaa
     5401 tttaatgata aacttcatgg cggcaatggt tcgttggcaa gccttaagcg acttgtatag
     5461 ggaaaaatac agcagcccac acctgcggct gcatccaggc gcggaagtat accactaaca
     5521 tcgctttgct gcgcacatca ccttaccatt gcgcgttatt tgctatttgc cctgagtccg
     5581 ttaccatgac ggggcgaaaa atattgagag tcagacattc attatgccga agaaaaatga
     5641 ggcgcccgcc agctttgaaa aggcgctgag cgagctggaa cagattgtaa cccgtctgga
     5701 aagtggcgac ctgccgctgg aagaggcgct gaacgagttc gaacgcggcg tgcagctggc
     5761 acgtcagggg caggccaaat tacaacaagc cgaacagcgc gtacaaattc tgctgtctga
     5821 caatgaagac gcctctctaa ccccttttac accggacaat gagtaatgga ctttccgcag
     5881 caactcgaag cctgcgttaa gcaggccaac caggcgctga gccgttttat cgccccactg
     5941 ccctttcaga acactcccgt ggtcgaaacc atgcagtatg gcgcattatt aggtggtaag
     6001 cgcctgcgac ctttcctggt ttatgccacc ggtcatatgt tcggcgttag cacaaacacg
     6061 ctggacgcac ccgctgccgc cgtagagtgt atccacgctt actcattaat tcatgatgat
     6121 ttaccggcaa tggatgatga cgatctgcgt cgcggtttgc caacctgcca tgtgaagttt
     6181 ggcgaagcaa acgcgattct cgctggcgac gctttacaaa cgctggcgtt ctcgatttta
     6241 agcgatgccg atatgccgga agtgtcggac cgcgacagaa tttcgatgat ttctgaactg
     6301 gcgagcgcca gtggtattgc cggaatgtgc ggtggtcagg cattagattt agacgcggaa
     6361 ggcaaacacg tacctctgga cgcgcttgag cgtattcatc gtcataaaac cggcgcattg
     6421 attcgcgccg ccgttcgcct tggtgcatta agcgccggag ataaaggacg tcgtgctctg
     6481 ccagtactcg acaagtacgc agagagcatc ggccttgcct tccaggttca ggatgacatc
     6541 ctggatgtgg taggagatac tgcaacgttg ggaaaacgcc agggtgccga ccagcaactt
     6601 ggtaaaagta cctaccctgc acttctgggt cttgagcaag cccggaagaa agcccgggat
     6661 ctgatcgacg atgcccgtca gtcgctgaaa caactggctg aacagtcact cgatacctcg
     6721 gcactggaag cgctagcgga ctacatcatc cagcgtaata aataaacaat aagtattaat
     6781 aggcccctga tgagttttga tattgccaaa tacccgaccc tggcactggt cgactccacc
     6841 caggagttac gactgttgcc gaaagagagt ttaccgaaac tctgcgacga actgcgccgc
     6901 tatttactcg acagcgtgag ccgttccagc gggcacttcg cctccgggct gggcacggtc
     6961 gagctgaccg tggcgctgca ctacgtctat aacactccgt ttgaccaatt gatttgggat
     7021 gtggggcatc aggcttatcc gcataaaatt ttgaccggac gccgcgacaa aatcggcacc
     7081 atccgtcaga aaggtggtct gcacccgttc ccgtggcgcg gcgaaagcga atatgacgta
     7141 ttaagcgtcg ggcattcatc aacctccatc agtgccggaa ttggtattgc ggttgctgcc
     7201 gagaaagaag gcaaaaatcg ccgcaccgtc tgtgtcattg gcgatggcgc gattaccgct
     7261 ggcatggcgt ttgaagcgat gaatcacgcg ggcgatatcc gtcctgatat gctggtggtc
     7321 ctcaacgaca atgaaatgtc gatttccgaa aatgtcggcg cgctcaataa ccatctggca
     7381 cagctgcttt ccggtaagct ttactcttcg ctgcgcgaag gcgggaaaaa agttttctct
     7441 ggcgttccgc ccattaaaga gctgctcaaa cgtaccgaag aacatattaa aggcatggta
     7501 gtgcctggca cgttgtttga agagctgggc tttaactaca tcggcccggt tgacggtcac
     7561 gatgtgctgg ggcttatcac cacgctgaag aacatgcgcg acctgaaagg cccgcagttc
     7621 ctgcatatca tgaccaaaaa aggtcgtggt tatgaaccgg cagaaaaaga ccccatcact
     7681 ttccacgccg tgcctaaatt tgatccctcc agcggttgtt tgccgaaaag tagcggcggt
     7741 ttgccgagct attcaaaaat ctttggcgac tggttgtgcg aaacggcagc gaaagacaac
     7801 aagctgatgg cgattactcc ggcgatgcgt gaaggttccg gcatggtcga gttttcacgt
     7861 aaattcccgg atcgttactt cgacgtggca atcgccgagc aacacgcggt gacctttgcc
     7921 gccggtctgg cgattggtgg gttcaaaccc attgtcgcga tttactccac tttcctgcaa
     7981 cgcgcctatg atcaggtgct gcatgacgtg gcgattcaaa agctcccggt cctgttcgcc
     8041 atcgaccgcg cgggcattgt tggtgctgac ggtcaaaccc atcagggcgc ttttgacctc
     8101 tcttacctgc gctgcatacc ggaaatggtc attatgaccc cgagcgatga aaacgaatgt
     8161 cgccagatgc tctataccgg ctatcactat aacgacggcc cgtccgcggt gcgctacccg
     8221 cgcggtaacg cggttggcgt ggaactgacg ccgctggaaa aactgccaat tggcaaaggc
     8281 attgtgaagc gtcgtggcga gaaactggcg atccttaact ttggtacgct gatgccagac
     8341 gcggcgaaag tcgctgaatc gctgaacgct acgctggtcg atatgcgttt tgtgaaaccg
     8401 cttgatgaag cgttaattct ggaaatggcc gccagccatg aagcgctggt caccgtagaa
     8461 gaaaacgcca ttatgggcgg cgcaggcagc ggcgtgaacg aagtgctaat ggcccatcgt
     8521 aaaccagtac ccgtgctgaa cattggcctg cctgacttct ttattccaca aggaactcag
     8581 gaagaaatgc gcgccgaact cggcctcgat gccgccggta tggaagccaa aatcaaggcc
     8641 tggctggcat aatccctact ccactcctgc tatgcttaag aaattattca tggcaggagt
     8701 ggcagcatgc aatacaaccc cttaggaaaa accgaccttc gcgtttcccg actttgcctc
     8761 ggctgtatga cctttggcga gccagatcgt ggtaatcacg catggacact gccggaagag
     8821 agtagccgtc ccatcattaa acgcgcgctg gaaggcggca taaatttctt tgataccgcc
     8881 aatagctatt ccgatggcag cagcgaagag atcgttggtc gcgcactgcg ggatttcgcc
     8941 cgtcgtgaag acgtggtcgt tgcgacgaaa gtgttccatc gcgttggtga tttaccggaa
     9001 ggattatccc gtgcacaaat tttgcgctct atcgacgaca gcctgcgccg tctcggcatg
     9061 gattatgtcg atatcctgca aattcatcgc tgggattaca acacgccgat cgaagagacg
     9121 ctggaagccc tgaacgacgt ggtaaaagcc gggaaagcgc gttatatcgg cgcgtcatcc
     9181 atgcacgctt cgcagtttgc tcaggccctg aagctacaaa aacagcacgg ctgggcgcag
     9241 tttatcagta tgcaggatca ctacaatctg atttatcgcg aagaagagcg cgagatgctg
     9301 cctctgtgtt atcaggaggg cgtggcggtg attccgtgga gcccgctggc gcgggggcga
     9361 ctgacgcgtc cgtggggaga aactaccgca cgactggtgt ctgatgaggt ggggaaaaat
     9421 ctctataaag aaagcgatga aaatgacgcg cagatcgcag agcggttaac gggcgtcagt
     9481 gaagaactcg gtgcaacacg agcacaagtt gcgctggcct ggttgttgag taaaccgggc
     9541 attgccgcac cgattatcgg tacatcgcgg gaagaacagc ttgatgagct gctgaacgcg
     9601 gtggatatca ctttgaagcc agaacagatt gccgaactgg aaacgccgta taaaccgcat
     9661 gcggtcgtag gatttaaata agagtccagg cctgatgaga cgtgacaagc gtcacatcag
     9721 gcatcggtgc acaactacga cagaataccc agcggccagt gatgaccgat aaagtacagg
     9781 atgcctgcgg aaatcacccc ggcgacaata tcgtcgatca tgatccccat gccgccatgt
     9841 acattgcgat caaaccagcg gatcggccac ggcttccaca tatccagaat acggaaaatc
     9901 acaaacccgg cggcaaccca ctgccagtca ttggtcggca gcgccatgag cgtgatccac
     9961 ataccaataa attcgtccca gacaatgctg ccgtgatcgt gcacgcccat atctttcgcc
    10021 gtttgatgac aaagatagac gccgatacag atccccaaca tcaccaccag cgagtagagc
    10081 tgccagggta aaaaggtcat cagataccag aacggaatcg ctgccagcga tcccatcgtg
    10141 ccaggaacga ttgggcttaa tccacttccg aatccgacag caagcagatg ccacggatta
    10201 ctcatcttca ggcgactttt cgcgacatct ttatggcgtg gcaaaatggt catatccttt
    10261 ccagtcaaac gtgacaggtt cgccgtcacg aataaaacaa agcccttcga tatcggcggt
    10321 catttgcccg atacaggtaa acggtacgcc aaggtgtccg agagcaacat ccagcgcgcc
    10381 acggttcagt tccggcaccg tgaaacacaa ctcgtaatct tcaccgccag agagcgccca
    10441 gcgcagcgcc tgttccggtt caacatggcg agaaagcgca tcagaaaacg gcagcaatcc
    10501 caggtcaata cgtgcgccgc agtcgctggc tttcacgata tgcccgagat cggaaatcag
    10561 accgtcagag agatcgatag ctgaatttgc cagatcgcgc agtgcctgcc cctgtaaaat
    10621 acgcggcgat ggacggagat gacgtttgat caagtagtca gcatctttag catcggcaac
    10681 ctgcaaacgg ttttgcaaaa tcgccagccc ggcggcgcta tcgcccggtg taccggtcac
    10741 atagatccaa tcacccggtt tcgccccaga gcgcgttaag gctcgtccca tcggaacaaa
    10801 gccgtggata cccaacgtca ttgataatgg cccacgcgtg gtatcgccgc caatgagttg
    10861 catatcgtaa taattgagaa gatcaaacaa actgtcgctg aaggactcaa gccacgcttc
    10921 gtctacgtcc ggtaaggtta atgccagcgt cagccaggcc ggatcggccc ccatcgctgc
    10981 cagatcgctt aggttcaccg ccagtgcttt ataagccaga tcagcaggat cgatatcagg
    11041 gaggaaatgg ttacccgcca ccagcgtatc agtgctgatc gccagggtct gtttctcggg
    11101 gatattgaga agtgcgcaat cgtcgccgat gcccagttcg acatcaagac gagaacttct
    11161 tacacggtca aaataacggg caatcaggga gaactcgcca catgccatac gttatgcctc
    11221 agcaggtaaa aaagaaaagg ccggcgatcg cggaacggtc ttccgtgaat ctaccggcct
    11281 ggatatcact ttttgttagg gcgaatcaca ggtgctgctt tatcgagtac gccgttgacg
    11341 aacttatggc tgtcttctgc gccgaacgat ttcgccagtt cgatcgcttc gttaatggcc
    11401 actttgtatg gcacatcgct acgtttagac agctcgtaca gcgcaatgcg cagtactgct
    11461 ttttctacct ggcccagttc ttccagcagg cgggacaggt atggcttcat cagtccgtcg
    11521 aggtatgcgg tgttagtcgc caccccggcc agcagctcac ggaagtacag gacgtcaacg
    11581 tcttttacat cctgttcagc caggaactgg tattcaacat cagcgatgtc gttctgggac
    11641 aactgccagg agtagagcgc ctggacggca cactcacgag cgcggcgacg agcagcaggt
    11701 ttcacggatt tccccttact aatttcaggc cttgatggct ttcaatacat taatcatttc
    11761 aagcgcggtc agtgcagctt ctgcaccttt gttgccagct ttggtgccag cacgttcgat
    11821 cgcttgttca atgctttcag tggtcagaac cccaaaagca accggaattt cgctgtcctg
    11881 ggcaacatgc gccaggccgt tgcttgcacc accagcgaca tattcaaagt gggcagtgcc
    11941 accacgaata accgtaccca gcgcaatcac cgcgtcgtat ttaccggttt tagccagtgc
    12001 acccgccgcc agcggcagct cataggcacc aggcacccaa acaacggtaa tgttttcatc
    12061 ttttacctga ccgatacgtt tcagtgcgtc aattgcacct tccagcaggc tgtcattgat
    12121 aaagttgttg aaacgcgcga tggtgatggc gacgcgagcg tccggggtag caacgttagc
    12181 ttcaataatg ttcattttct tccttcgggt tcgagtatgg ccccgcaggg gggcggattt
    12241 tagcataata tttcgtgcgc tgcttccctt tcgagccggg agatcatgca cccactaaat
    12301 gcaggcaaac atccgggcct acatgacgta tctctttgaa tttaaattgg ggggcgtcgg
    12361 ctaatttctc aagccctggc agcgagcata atccacgggc gtcgctgcct aatagtttag
    12421 gtgcgatata gacaatcagc tcatcgacta aacccacctg cagcaatgcg ccagcgagcg
    12481 ttggccccgc ttccacccag atgctgttaa tttgctgttt acccagttgc atcatcagta
    12541 caaccagatc cagatgacct ttatgctctg gaatcagcaa ggtacgcacc gtttccggcc
    12601 actcacgaga atcttcctgc gtacgcgcga accaggtttc gccgggctgc tgcacaatgc
    12661 gatgttccgg cgtcacgcga ttttggctat caatcacaat acgtaccggc tgacggaaac
    12721 tttgttgcgg gtagagcgcc tgagtttgtt catccagttc ggaccaacgc accgttaagg
    12781 caggatcatc cgccagcacc gtggcgctgc tggttaaaat ggcatgactt tgcgcgcgca
    12841 gtcgttgtac atcacgccgc gcctggggcg aagtgatcca ctggctttcg ccgctcgcca
    12901 tcgccgtgcg accatcaagc gatgcgccaa gttttaactg aatataagga aagccggtgc
    12961 gcatccgctt gagaaagcct ttattcaatt gctcgacttc actcatcatc agcccgtggc
    13021 tgacgtcaat gccagactgt tgcagacggt aaagtccacg cccagcgacc tgcgggttag
    13081 gatcttgcat cgctgcgacc acgcgcgcta cgccagcggc gatgagtgcg tcacagcacg
    13141 gtggcgtacg accatgatgg ctacagggtt cgagtgtgac ataggcggtc gcacctttgg
    13201 ctttttcacc cgccatacgc aacgcgtgta cttcggcatg tggttcaccc gctcgttggt
    13261 ggtaaccttc accgacaatt tcgccatctt tgacaatgac gcacccgaca ttcgggttgg
    13321 ggtgggtggt aaaacgtcct cgttgcgcca gctttagcgc ccgcgccatg taatactcgt
    13381 cctgcacggc ttagtcctcc aggcgcgcga tctcttcgcc aaattctttg atatcttcga
    13441 aactgcggta gacagaggca aaacggatat aggcgacttt atcgagcttt ttcaattgct
    13501 ccatcaccag attgccaatc atcttgctcg gtacttcgcg ctcaccggtg gcgcgcagct
    13561 gcgatttaat atgattgatt gccatttcaa cgtcatcgga actcaccgga cgtttttcca
    13621 gcgcccgcag cattccgctg cgcaatttct cttcattaaa cggttcacgc acgtcgttgc
    13681 tttttacaac acgcggcata accagctccg ccacttcaaa ggtggtgaaa cgttcattac
    13741 acaccagaca ctgccgacgg cggcgtacgg atgaaccctc gcccacgaga cgagagtcaa
    13801 ttaccttagt gtccacggcg aaacagaatg ggcaatgcat acggcgtcct gaccaggtgg
    13861 ttaacggaaa tctattttac cctgaactga cgatacaaca aaggcgcaac gcttttgaga
    13921 taaaggatcg atgctttcgc tgcttttacc ctctaccatt aggctaattg cgacgagaaa
    13981 gggacgacct atgaatacta acgtttttcg actgctcctg ctgggaagcc tgttcagcct
    14041 tagcgcctgt gtgcagcaaa gcgaagtgcg acagatgaaa cacagcgtca gcacgctgaa
    14101 ccaggagatg acgcaactca atcaagaaac agtcaaaatc actcagcaaa acaggctgaa
    14161 tgcaaaatcc agcagtgggg tttaccttct gcctggagcg aaaacaccgg caagactgga
    14221 aagccggatt ggtactttac gtatgtcgct ggtgaatatt acgcctgata cagatggcac
    14281 caccctgacg ctacgtattc agggcgagtc gaatgatccc ttgcccgcat tcagcggaac
    14341 cgttgaatat gggcaaattc agggaacaat agacaacttt caggaaatca atgtgcagaa
    14401 tcaattgatt aacgcccctg ccagcgtcct cgcccccagc gatgttgata ttccgttaca
    14461 gttaaagggg attcctgtag aacagttaga ctttgtacgt atccacgaca ttcaacctgt
    14521 tatgcagtaa acgtatttcg gggcgatttt gtgcgtctcg caacatcttt ccccgtcatt
    14581 ttgttactcc gcttacatca ccctgattga tagtaaaagt ttgcaacaag ggtgaaagtc
    14641 agtacaatcc ctgcccgaat gtgtgtaaac gtgaacgcaa tcgattacgt aaatgataga
    14701 actgtgaaac gaaacatatt tttgtgagca atgattttta taataggctc ctctgtatac
    14761 gaaatattta gaaacgcaat ttgcgccttt ttcactcccg caagggattt tcaaacagtg
    14821 gcatacatat gaaaaaaaca ttactggcag ccggtgcggt actggcgctc tcttcgtctt
    14881 ttactgtcaa cgcagctgaa aacgacaaac cgcagtatct ttccgactgg tggcaccaga
    14941 gcgttaacgt tgtcggaagc tatcacaccc gtttcggacc gcagatccgc aacgatacct
    15001 accttgagta cgaagcattc gctaaaaaag actggttcga cttctatggt tatgcggatg
    15061 cgccggtatt cttcggcggt aactccgatg ctaaaggtat ctggaaccac ggttctccgc
    15121 tgtttatgga aatcgaacca cgtttctcca tcgacaagct gaccaatact gaccttagct
    15181 tcggtccgtt caaagagtgg tacttcgcga acaactacat ttacgacatg ggtcgtaata
    15241 aagatggtcg ccagagcacc tggtacatgg gtctgggtac cgacatcgat actggtctgc
    15301 cgatgagcct gtccatgaac gtctatgcga aataccagtg gcagaactat ggcgcagcga
    15361 acgaaaacga gtgggacggt taccgtttca aaattaaata ctttgtgccg attaccgatc
    15421 tgtggggcgg tcagctgagc tacatcggct tcaccaactt cgactggggt tccgatttag
    15481 gggatgacag cggtaacgca atcaacggta ttaagacccg tactaataac tctatcgctt
    15541 ccagccatat tctggctctg aactacgatc actggcacta ctctgtcgta gctcgttact
    15601 ggcacgacgg tggtcagtgg aacgacgatg cagaactgaa cttcggcaac ggcaacttca
    15661 acgttcgctc taccggctgg ggtggttacc tggtagtagg ttacaacttc tgattatgaa
    15721 aatgccggga tttattcccg gcatttcaga ttgttaacaa cgtgcgcttt gttcatgccg
    15781 gatgcggcgt aaacgctttg tccgacccac aaaagcacgt aaattcaata tattgctgaa
    15841 ataacgtagt cctgataagc gtagcgcatc aggcaatttt acgttttaat cacttcttct
    15901 tgttcatcat cgctttcagg tcagcgaatg ggttgtactt cgcttcaccg acatctttct
    15961 gcgcatcttc ccctgcgata acggtcgtac catactgatc cgcttcggta tatttcgaat
    16021 gctcatgatc gtggcaatag agacaaagca attcccagtt actgccatct tccgggttat
    16081 tggtatggtc gtgatcaatg tggtgaacag taagttcacg caggttggaa taaacaaact
    16141 cgcgggaaca gcgaccgcag acccacggat atatttttaa tgctttttcg cgatagccgc
    16201 tttctaaccg cgcatagttt tttgggatga tagccatgaa gagttacctg gatgaaatgt
    16261 agggttacgc gcagtttccc aagatgcggg gattttagca atctccatac atcaaaaaaa
    16321 atcccgatct tctgaccggg attttcaaca tcaacgggaa cttgattacg gcagaattga
    16381 cggctgatcc gccccttctt tttccacttt ctgctgcaac atgtgttcgc gcttcatacc
    16441 cagtttcaga gccaacgcag atgccacata gatggaagat gcagtaccga tggaaacacc
    16501 gataagcatg gtcagcgaga agccttccag taccggacca ccgaagaggt acagcatcag
    16561 gataaccatc aaggtagtac cggatgtgat caaggtacgg tgcagcgtct gggtcaagga
    16621 cacgttaaag atttcgtaag gcgtaccgcg acggatcttg cggaagtttt cacgaatacg
    16681 gtccgatacc acgatactgt cgttaagcga gtaaccgata accgacatca acgatgccac
    16741 aatggtcagg tcaatctcga tatggaataa cgacaaaata cccagcgtaa tgataacgtc
    16801 gtgcgccagc gcaataacga cccctgccgc cagtcgccac tcaaagcgaa aacctacgta
    16861 cacaaggata gacagcagcg ccgccatcaa cgccatcgca ccggtttgcg caaggtctgc
    16921 ccccacgctc ggaccgacga actcaatacg cttcactgct gcattctgat tggtggattc
    16981 gttaatcacc ttcagaacct ggctgcccag cacctgaccg ccggtttcgc cttcagcagg
    17041 cggcatacgg accatgatgt cgtggctgct accaaagttt tgcagcattg gctcttcaaa
    17101 accggctttt tgcaatgcat cacgcattac gtcaatttca gccggttttt cgagcgtaat
    17161 ttcgataacc gtaccaccgg tgaaatccag cccccagtta aagccgcgca cgcccataat
    17221 aacgatagcg gcgattaaca gcagaccaga gatgccgaaa gcccagtagt cccagcgcat
    17281 aaagtcatag actttacggc cgtggtttag ttgttcaaca gtatattcct gtgccacatc
    17341 gcactcctca gattgacagc ttcttgacgc gcttgccgcc atatagcagg tttacgatgg
    17401 cacgcgtacc gacaatcgcg gtaaacatcg acgtcgccac accgataccg gtagtaatcg
    17461 cgaacccttt aattgccccg gtacccactg cgtacaggat gatgacttta atcagcgtgg
    17521 tgatgttcgc atcgaagata gaactgaatg cgccacgata accttcatca attgcctgtt
    17581 gaacagtacg tccgttgctc aactcttctt taatacgttc gttgatcagt acgttcgcat
    17641 cgaccgccac cgcaagggtt aagacgatac ccgcgatacc tggcatactc agcgttgcgc
    17701 ctggcaacag cgacataatg ccgacgatta agatcaagtt ggcaatcaga gcactggtcg
    17761 caatcagacc aaacttctta tagaagatga tcatgaacag aatagaaacc agcagaccgg
    17821 caaggcaagc ctccagcccc tgttcaatgt tctgcatacc cagggttggg ccaatggtac
    17881 gttcttcaac aatctgaatc ggcgcgatca acgcaccggc acgcagcagc agtgacagct
    17941 gacgggcttc gttcgggttg ttgatgccgg tgatacggaa gctgttaccc agacgagact
    18001 ggatgttggc gatgttaatc acctcttcct gtttcaccag aaccgcacga ccatttgcat
    18061 ctttcttacc gctgtctttg tactccacaa acagggttgc catcggtttg ccgatgttgt
    18121 ccttagtgaa gttagacatg atgttaccac cagcgctatc gagcgagatg ttaacctgcg
    18181 gttggttgta ttcgtcctgg ctggaagtgg agtcggtgat atggtcaccg gtcagaatta
    18241 cgcgtttgta cagcacaact ggctgacctt cgcgggtctg tttcacttca gagtcgcccg
    18301 gtacgcgacc ggatgccgca gcggcctggt caacgttggt gtttaccaga cggaattcca
    18361 gcgttgccgt cgcacccaga atctctttcg cacgcgcagt gtcctgaata cctggcagtt
    18421 caacaacgat acggtcagca ccctgacgct gaaccaccgg ctccgccacg ccaagttggt
    18481 ttacacggtt acgcaggata ttgatgttct gctgcaccgc atattcacgc gcttcactca
    18541 gacgagcatc gctcattacc gcacgcagct ggttgctgcc ctggctacta atcaccaggt
    18601 ccggatggcg cttgctcaga tacgcaatgg cttcatcacg agctttagca tcgcggaaag
    18661 taatgctcag gccgtagttg ttttctttac gaacggtggt atacgggatg cccttttcac
    18721 gcagatcgct gcgcaggcta tcgatatttt gttcctggag tttgccaagc gcggtgtcca
    18781 tatccacttc catcaggaag tgaacgccgc cacgcaggtc aaggccgagc ttcatcggct
    18841 cagcgtgaat agctgccagc cagcgcggcg ttgccggggc aaggttaagc gccacgacgt
    18901 atttgtcacc cataacgccc attaatgctt cacgagcgcg cagctgggtg tcagtggagt
    18961 cgaagcgcgc aagaatagcg ccctcttcca gtgccacaga cttagcagtt attttttctt
    19021 cttgtaacgt tttctggacc tggatcagcg tttgctcact ggcggcgact ccgcgcgcac
    19081 cagtgatctg aacagccgga tcctcaccaa acaggttggg aagcgcatac agcagaccga
    19141 tgacaatcac cacgatcagc atgacgtact tccacaaagg ataacggttt aacacggcaa
    19201 ttcccttagg gaaaaatttt aattacagcg ccttcatggt gcctttcggc aggacggcag
    19261 ctacgaagtc acgtttaata actacttcag tggtgtcatt cagcgcgata gcaatgtagc
    19321 cgttttccgc tactttggtt acgcgaccaa ccaggccacc gttcgtcaga acttcatcac
    19381 ctttggcaat ggagtccatc agctttttgt gttctttggt gcgcttctgc tgtggacgca
    19441 ggatcatgaa atagaaaatc agaccgaata ccaccagcat caaaatcaaa gacatcgggc
    19501 taccttgcgc cggtgcaccc gttgccgcta ccgcatcaga aataaaaaag ctcattaaat
    19561 ttccctcatt attaatatta atcaacgttc aaaggtggta cttctcgccc ctgacgctgg
    19621 taaaaatcag ttacgaagct ctctaattta ccctcttcaa tagccttgcg taaacccgcc
    19681 atcaaacgct ggtagtaacg aaggttatga atggtgttga gtcgcgcgcc taatatttcg
    19741 ttgcaacggt caagatgatg caagtaagcg cgtgaataat tgcgacaggt gtagcaatca
    19801 cactcaggat cgagtgggcc agtatcgctc ttatacttcg cattgcggat tttcaccacg
    19861 ccatcggtca cgaacaaatg accatttcgg gcgttgcggg ttggcattac gcagtcaaac
    19921 atatcgatac cacgacgtac gccttcaacc aggtcttctg gtttaccaac gcccatcagg
    19981 taacgcggtt tgtctgccgg aatttgcggg catacatgct ccagaatgcg gtgcatatct
    20041 gctttcggct cacccacagc cagaccgccg acagcgtagc catcaaaacc gatatctacc
    20101 agacctttaa cagaaatatc acgtaaatct tcgtaaacgc tgccctgaat gataccaaac
    20161 agcgcatttt tgtttccgag actgtcaaaa cgctcacggc tacgcttcgc ccaacgcaga
    20221 gacatctcca tggagcgttt tgcgtaatcc cagtcagcag gatacggcgt acactcatca
    20281 aagatcatga cgatatccga accaagatcg tactgaatct ccattgattt ttcaggatcg
    20341 aggaaaatcg gatcgccgtt gatcgggtta cggaagtgca cgccctgttc ggtgatttta
    20401 cgaatatcgc caaggctgaa gacctggaag ccgccggagt cggtgaggat cggccccttc
    20461 cactgcataa aatcgtgcag atcgccgtgc agtttcatga tttcctggcc cgggcgcagc
    20521 cacaggtgga aggtgttgcc gaggataatt tgcgcgccag tggcttcaac ttcttccggc
    20581 gtcatccctt ttacggtgcc gtaggtgcca acaggcataa aacaaggcgt ttccactacg
    20641 ccacgatcaa agaccaggcg gccacggcgt gcgcgaccgt cggtggtgtc cagttcaaat
    20701 ttcatttttt ctccactacg tcagaaaaac agtccaacgt tttaaaccag cgccgcggaa
    20761 ttactccccg acgcgctcat taattgcctg cggattgtac gtgataaaca tcgcatcacc
    20821 gtaactaaaa aagcgatatt tctcttctac cgctgcttta taggcattca tggtgtgttg
    20881 ataaccggca aaggccgaaa ccagcataat cagcgtcgat tccggcaggt ggaagtttgt
    20941 caccagcgca tcgaccactt tgtactggaa gcccggatag ataaagattt gggtatcgtc
    21001 aaagaacggt tcgataagat cgtttttcgc cgcctgagcc gcgctttcca gcgaacgtac
    21061 tgaggtggtg ccaaccgcaa tcacccggtt accgcgcgct ttcgccgcca gtaccgcgtc
    21121 taccacatcc tgcggcactt cagcgtattc cgagtgcatg atgtgatctt caatggtgtc
    21181 gacgcgcacc ggctggaagg tgcccgcacc aacgtgcaac gtcacaaacg ccatctccac
    21241 gcctttggcg cgtaattttt ccagcaaagg ttcgtcaaaa tgcagacctg cggtcggggc
    21301 tgcaaccgcg cccggttttt cgctataaac ggtttgataa agttcgcggt cagcgtcttc
    21361 gtccggacgg tcgatatacg gcggcagcgg catatggccg atgctgttga gaatatccag
    21421 caccgagcgt tcatcattaa attcgacttc aaacagtgcg ccgtggcgcg cggtcattgt
    21481 tgcgttaata ctttcgtcat cgcccagcag cagttctgcg ccaggttttg gcgctttcga
    21541 ggcgcgaata tgcgcaagaa tgcgtttgtc gtcgagcatc cgttcaacca gcacttcaat
    21601 cttgccgccg ctggctttac gcccaaacag gcgcgccggg atcacgcggg tattattaaa
    21661 aaccagaaga tcgccggggt tgagcttatc aagtaaatcg gtgaaagtac cgtgcgtcag
    21721 cgcgcccgtc ggcccgtcca gcgacagtaa acgacagcta ctgcgttcag gcatgggata
    21781 gtgggcaatc agggattcgg gcaattcaaa ggagaaatcg gtaacgcgca tgacactgac
    21841 tcagactaaa ataagaggcg ggtagtctag tgccggggcg ctctccctgc aacctttacc
    21901 cccttttgga tagaatataa tcgatgaatt ttttagctca cctgcattta gcccatctcg
    21961 cggaaagctc gctttccggc aatttactgg ctgatttcgt acgcggaaat cctgaagaaa
    22021 gttttccgcc cgacgtcgtg gctggcattc atatgcatcg acgtatcgac gtattaaccg
    22081 acaatctgcc ggaagtccgc gaagcacggg aatggtttcg tagtgaaact cgccgcgttg
    22141 cgcctattac gctggatgtc atgtgggatc actttctttc ccgccactgg tcgcagctgt
    22201 cgccggactt tccgctacag gaatttgtct gttatgcccg cgagcaagtg atgacgattt
    22261 tgccggactc accgccacgt tttatcaatc tgaacaatta cttgtggtca gagcagtggc
    22321 tggtgcgcta tcgcgatatg gatttcatcc agaacgtgtt aaacggcatg gcaagccgcc
    22381 gcccacgtct ggatgccctg cgtgactcct ggtacgattt agacgctcat tatgacgccc
    22441 tcgaaacccg cttctggcag ttttatccgc ggatgatggc gcaggcgtca cacaaggcgt
    22501 tataaaacgt tagttcatcc ataccgtagc cgaaatggca ggcaaagcca gaatcccgtc
    22561 agtcagttgc ccatgccctt ctttgcattg ccattgcacg gcattgagca acggtgacgc
    22621 gggtagcacg acttcacaag cttcgccacg gttgattgcc accagtacac gctgctgatt
    22681 cagcacgcgg acaaatacca ccacgttatc ttccgcatac agcacctgac agccgccatg
    22741 acgtagcgcc tgacttttct tacgcagcgc aatcattcgc tggtacagcg cgaataacgc
    22801 cgtatcctgc ttttccacct gccaagggaa cggtttacgg caaaacggat cgtttttgcc
    22861 atccagtcct acttcatcac cgtaataaat gcacggtaca ccaggccagg tgaacagcca
    22921 gaccaccgcc agcggcaggc gcgcaatatc ccgaccgagc agcgttttaa atcgcgcagt
    22981 atcgtggctg tcgagctgat taaacatacg taattgttgt tgatgagaaa gccctgcgcg
    23041 gtaattatcc atccaggcca tacaggtttg ggcatcaatt tgctgcggat cgtaagagat
    23101 atcggtattg gcaagaaatc cccacaacgg gaatgtgaag ccacgatagt tcatggcggc
    23161 atcttccaca tcggcctgta accattgccg tgcatcgcca aaatgttcgc cgacaatata
    23221 cgcttccggc tgggtttctt tcgccgcttc ggtgatccca gcaacgtgct gcatattatt
    23281 gcgcgcccca cccgcctccc ccagcatatg caccacatcc agccgccagc cgtccatact
    23341 ccacggcgct ttcagccaat ggcggacaat actgtcttcc ccgcgataaa tttcattcac
    23401 cagactttcc gactgataat ccagcttcgg caagctggca tagccaagcc agtcgagcgc
    23461 cgtgccatca tcactaaacg agtaccagtc gcgccagggc gattcagggt tgtgacaagc
    23521 tccccccgtg ccacgattgt gcctgtcaaa ccaggcatgg gaatcgccac tgtggttaaa
    23581 cacgccgtcc agcaccagcc gcattcccag ctgctgcgta ttgtgtcgca aacgcagcaa
    23641 cgccccatca ccgccaaact gcggatcgac atggcgataa tcctcggtat cgtatttatg
    23701 tacgctggga gctttaaaca ccggattgag atacagcgcc gtcacgccaa gctttttcag
    23761 atacggcagt ttttcgctta tcccgtccag atcgccgcca tagaacgttg atccgcccgc
    23821 ctgcgccgtg accggttcat cccagtcacg caagatgatc tcttgtccgg ctgcatgatg
    23881 gtaatagaca tgatcctgtt cagcttcacg aggaagacta cgcgcaaaac gatcagggaa
    23941 gatctgataa aaaatctgat ccgcagccca ttgtgggccg atatccggta catcgacggc
    24001 aaactgctcc agtcgtgccg gcggcattcg gctgaagccc tgcggtgtaa accaacgctg
    24061 gcgatcgtgc cacagcagtt tgaaactgta acgccgccgg ggttgtccgc tggagagatc
    24121 aatcgccgca cgccatgcgg tgacgccagg ctgctgctga ctgcgctgct tatgcatcga
    24181 tactgacatt tcttcgttat cgtgttctgt acgcagcata atgcgctgcg gtgggtcttc
    24241 gcccgtcagc cacagtgtaa tgagcagttg atctttgctt tgtttaacaa atgggggcac
    24301 cggcaggtgc catgcattta acatcataat tcccctgtga tgaaaatctg atcaccttgc
    24361 catagcctaa tgcaggatca ctcatcatct gaaggatatt tgggggagga ggacgggggc
    24421 ggataacttt cggaatagcc ggatcaggat ttcctcctga accggcttac agaacgcaag
    24481 tattattggt tttcagccag ctgacgatcg tggcggcgtt taaacatcca gccaatcaac
    24541 agcacaacaa tccacgcgaa accgacatac agcgagatac gcgtatccgg gtgataacca
    24601 atcaacccga taataaagag caggaaaatc agcccgccga tggtcgttgc taccccaccc
    24661 ggcactttaa atttcagcgc cttaacttct tctggcggca aacggcgacg gaaggcaatt
    24721 tgcgacagca ggatcataat ccacacccac accgtggcga aggttgccag cgaagcgatc
    24781 accaggaaga cgttttccgg catgatgtag ttcagataca ccgcaaacag cagcgcggta
    24841 gtcatcacca gcaccgtaac ccacggaata ccgcgacgcg acgttttgct gaaaattttc
    24901 ggcgcgctgc cctgctctgc cataccgtgg agcatacggc ctacgccaaa tacatcactg
    24961 ttaattgccg acagcgaagc agtcagcaca acaaagttaa gaatgctggc ggcaaaggta
    25021 atgcccatat gctggaacgt cagcacgaac gggctaccgg cagtgccaac ctgattccac
    25081 gggtagatag acataatgac gaacagcgta ccgacgtaga acaccagaat acgcatcggc
    25141 acggagttaa tcgcacgcgg tatcgatttc tcaggatctt tcgcttcacc ggcggtaatc
    25201 ccgataattt cgatcccacc gtaagcaaac atcaccattt gcaacgacat taccatgcca
    25261 agccagccgt tactgaagaa gccgccgttg ctccacaggt tatgaatacc ggtcggttgc
    25321 ccgccgttgc caatccccca gatgatgatg ccgaaaccgg cgacaatcat gatgatgatg
    25381 gtggcgactt taaagaacga gaaccagaat tccagctcac cgaagacctt cacgctcatc
    25441 aggtttacgg cgcaaatgat cagcaccacg ctcagtaccc aaatccagtg cggcaccgtc
    25501 gggaaccaga cacccatata gataccaaaa gcggtcacat cggcgatggc gacaataagg
    25561 atttcaaagc agtaggtcca tccggtaatg taacctgcca gcgggccgag gttttcctgc
    25621 gcataacgcg agaaagagct ggcggccggg ttatgtaccg acatttcccc cagcgcacgc
    25681 ataatgatat acgccgcgat accaccgata atataggcca acaacacgct cggaccggcc
    25741 attttgatgg cgtctgccga accgtaaaac agcccggtgc caattgctga acccagtgcc
    25801 ataaagcgta tgtggcgggt acttagccca cgctttagct tgttcttact ttccatcaat
    25861 tcctaaccca tcaacacaat aaaaaaccac ggggcatcgc agccccgtgg ttaaaacaaa
    25921 tgttcagtga tttagtgagc gctggaggtc acctgacgac ctgccgcgcg atcccagata
    25981 atggccagaa ccaccatcac cactgttggc attaaccacg ccagaccttg ttcggccagc
    26041 ggtaaacgct gcgcccaaga cggtaagata tcgctgaatg cagatgcttt gatcccgtcg
    26101 agaataccaa aaagcaggct gataaacatc ggcggagcaa tcacgcggga cgaattatgc
    26161 caccatgagc gtgtaaaact taatacaacc agtgcgatac acggcggata aatagcggtc
    26221 agcaccggta cggagatctg gatcagctgg cttaagccga ggttagaaac caccatcgag
    26281 aagccgccga ggataaacac cagcgtacga taagagagcg gtacgtactg ggcaaagaat
    26341 tctgcacaag cacaggtcag gccaactgcc gttaccaggc aggcgatgaa gattaacgcc
    26401 gccagcagga agctaccgcc gccgccaaag gtgtgctgaa cgtaagcatg cagaatagca
    26461 gcgccgtttg cagactgatc gaccagcgac gcgctgtctg aacccagacg gaacagcgcc
    26521 aggtacagca gagtcagacc aacacccgcc atcaggccag cccagacggt ataacgggtc
    26581 agcagacgcg cttcggtaac gccacgagaa cgcgccgcgt taacaataac gataccaaac
    26641 accattgcgc ccagcgtatc catggtcaga tagccgttaa cgaagccgtt agaaaacgca
    26701 gcgttttgat aagcctcagt cgccgtgctg atagaacccg ccggccaaac aatcgcggca
    26761 acagacagga tgaccagcgc gataattttc agcggcgcaa ggaagttgcc cacggtatcc
    26821 agcagcttgc ccggatagag cgaaaccaga ataacgatag cgaaatagac caggctgtag
    26881 ataaacagcg gcagcgcgga atcaccagtc agcggcgcaa tccccacttc aaaggaaacg
    26941 gtagctgtac gcggcgtagc gaaaagcggc cccaccgcca ggtaacaaac cgttgccagc
    27001 agcacgccag cgactttacc gattggcgtg ctgaggctgt caacaccgcc gccaactttt
    27061 gccagcgcca ctaccgttaa taccggcagg ccaacggcag taatgaggaa gccgaatgcc
    27121 gcagtccaga cgtgttcgcc tgcctgcaag ccgaccattg gagggaaaat aatgttacct
    27181 gcgccgacga acaacgcaaa tgtcataaag cccagagcga tgatatcgcg cgatcttaat
    27241 tgatgggtca taaaccatac tgcctgtgga tgtggtgttg taacgttaaa tttttgtgcc
    27301 aatccctggg gatgatacag cggtatattt aaacaatttt agcgggaaat actcccaacc
    27361 aggcgcggcg aagaatagtt tttcgattta ccgcgttaat gcagtcagta tgacagcacc
    27421 tgaagcgcaa tttaaacgct tataacgttt taaagcaagg agggatctaa aaaccagagg
    27481 tatatagcga taagaaatca ccaaccagta taaaacacta tttccgtgaa aaagacatgg
    27541 ctaatcatgc gaacaaaaaa tcaccaaccg ttgtaaaatc taacgcccat attttgactg
    27601 caaagaaaat aagccagcat cggctggctt atggcaataa aagatgacaa aggcggatta
    27661 atcgctgttt ttggcaatta aacgttccgg gataacaaaa ctgaaacgtg ttccttttcc
    27721 tactgtactc tcaatattca ggcgactttc gtgatgattc acagcatgtt tcacgatcgc
    27781 taaccctaat ccgctaccgc cggtttgccg ggaacgcgct ttatcaacgc gataaaaacg
    27841 ctcggtcagg cgcggaatat gctccgctgc aatgcccggt ccgttatctt caacgctaaa
    27901 ttcggcaccg tgcggcactc gctgccagcg tacggtgata tgcgtgcctt ccggcgtatg
    27961 attcacggcg ttatagacca ggttcgaaat cgcactgcgt agctgatctt cgttgccaga
    28021 caccttgagg ccgctatcta tctcaaaggt aaatgtctgt tttttctgac tcagagtctg
    28081 agcctcacgc tcaacaacgc gcagcatcat cggcacatca accttttcat tgagcaaatg
    28141 cgtcggtgcg gcttctattt tcgacagcgt cagcaactgc ttcaccagcc cttccatccg
    28201 ctgggtctgc tcgcgcatgg tgtgcaacgc tttttcgcgt accgcgcctt ccagcggctg
    28261 ctcatccatc atctccaggt aaccctgtaa cacggtcaat ggcgtacgta actcatggct
    28321 cacgttggca aaaaagttac gccgcgcccc ttccagttga tgcatttgcg tgacatcacg
    28381 cgccaccatc agcaactgtt tgtgggtata aggcatgacg cgaatttcca gatgccgccc
    28441 ggtgttgagc accagattga gcgggcgaga aaaatcacgc gttttcaaat attgcgtaaa
    28501 ctccgggtaa cgcagcaggt taaggatgtt ctgcccgtta tcttccggcc agcgcaaacc
    28561 aagaatctgt tgcgccagac cgttacacca gaaaataccg ccctcttccg tggtcagcac
    28621 caccgcgtcg ggcagcgact ccgcgccgct acgaaagcgt ttaatcagat tgcccagttc
    28681 acggcggcgt tttttatttc gcagctgcat ctggtgtaag ccgtatagta gcggttccca
    28741 gctaccacgc cccggtggcg gggtcatact gcgatccacc cacagccacc atgaaaggcg
    28801 caataaattc cagaaatgcc agataagcag tcctgttacc gatgccagca aaaaccaggg
    28861 caggtaacca aaaaatgcac ccaggatgaa agccgggagg cagcaaagta gcagctccag
    28921 caccagcctt ttccacgaca gccgttccag cacgtaagat actccagtta agaaatcata
    28981 agccctgctc tgcgtccgat gagcaaggcg ttaaaagcgg gttgaaaaac gatatcctgt
    29041 accgcgcacg gtctgcacca tgcgatcatg cccgcctggc tccagtgctt tacgcaggcg
    29101 acgaatgtgg acatcgaccg tacgatcttc cacatacacg ttggttcccc agacgtggtt
    29161 taacagctgc tcgcggctgt atacgcgctc aggatgcgtc ataaaaaagt gcagcagttt
    29221 aaattctgtc ggccccatct ccagcggctc ttcacccgcc atcacccggt gagatgtcgg
    29281 gtcgagactt agcccctgca tctcgatcac ctcttccacc gccattggcg aaatacggcg
    29341 cattaccgct ttgattcgcg ccaccagctc cttcggcgaa aacggcttgg tgatatagtc
    29401 atccgccccg gtttcaaggc cgcgcacgcg atcttcttct tcccctctgg cggtcaacat
    29461 caccactgga atatcccggg tcatcgactc gcgcttgagg tgtttgatga actggatacc
    29521 ggagccgcca ggtaacatcc agtcaaggag aattaaatcc ggccagggtt cattcagttg
    29581 attcacagca ctgtcataat cttccgcttc gaccggctga aagccatttt gttcgagcac
    29641 gaagcagacc atttcgcgaa ttggagcttc atcttctacg accagaatac gtctcgccat
    29701 gatttgccct gttgtaataa ataggttgcg atcattaatg cgacgtcatt atgcgtcaga
    29761 tttatgacag atttatgaaa agctcgtcgc acatatcttc aggttattga tttccgtggc
    29821 gcagaaaaaa gcaaatggca catctgtttg ggtataatcg cgcccatgct ttttcgccag
    29881 ggaaccgtta tgcgcatcct tcacacctca gactggcatc tcggccagaa cttctacagt
    29941 aaaagccgcg aagctgaaca tcaggctttt cttgactggc tgctggagac agcacaaacc
    30001 catcaggtgg atgcgattat tgttgccggt gatgttttcg ataccggctc gccgcccagt
    30061 tacgcccgca cgttatacaa ccgttttgtt gtcaatttac agcaaactgg ctgtcatctg
    30121 gtggtactgg caggaaacca tgactcggtc gccacgctga atgaatcgcg cgatatcatg
    30181 gcgttcctca atactaccgt ggtcgccagc gccggacatg cgccgcaaat cttgcctcgt
    30241 cgcgacggga cgccaggcgc agtgctgtgc cccattccgt ttttacgtcc gcgtgacatt
    30301 attaccagcc aggcggggct taacggtatt gaaaaacagc agcatttact ggcagcgatt
    30361 accgattatt accaacaaca ctatgccgat gcctgcaaac tgcgcggcga tcagcctctg
    30421 cccatcatcg ccacgggaca tttaacgacc gtgggggcca gtaaaagtga cgccgtgcgt
    30481 gacatttata ttggcacgct ggacgcgttt ccggcacaaa actttccacc agccgactac
    30541 atcgcgctcg ggcatattca ccgcgcacag attattggcg gcatggaaca tgttcgctat
    30601 tgcggctccc ccattccact gagttttgat gaatgcggta agagtaaata tgtccatctg
    30661 gtgacatttt caaacggcaa attagagagc gtggaaaacc tgaacgtacc ggtaacgcaa
    30721 cccatggcag tgctgaaagg cgatctggcg tcgattaccg cacagctgga acagtggcgc
    30781 gatgtatcgc aggagccacc tgtctggctg gatatcgaaa tcactactga tgagtatctg
    30841 catgatattc agcgcaaaat ccaggcatta accgaatcat tgcctgtcga agtattgctg
    30901 gtacgtcgga gtcgtgaaca gcgcgagcgt gtgttagcca gccaacagcg tgaaaccctc
    30961 agcgaactca gcgtcgaaga ggtgttcaat cgccgtctgg cactggaaga actggatgaa
    31021 tcgcagcagc aacgtctgca gcatcttttc accacgacgt tgcataccct cgccggagaa
    31081 cacgaagcat gaaaattctc agcctgcgcc tgaaaaacct gaactcatta aaaggcgaat
    31141 ggaagattga tttcacccgc gagccgttcg ccagcaacgg gctgtttgct attaccggcc
    31201 caacaggtgc ggggaaaacc accctgctgg acgccatttg tctggcgctg tatcacgaaa
    31261 ctccgcgtct ctctaacgtt tcacaatcgc aaaatgatct catgacccgc gataccgccg
    31321 aatgtctggc ggaggtggag tttgaagtga aaggtgaagc gtaccgtgca ttctggagcc
    31381 agaatcgggc gcgtaaccaa cccgacggta atttgcaggt gccacgcgta gagctggcgc
    31441 gctgcgccga cggcaaaatt ctcgccgaca aagtgaaaga taagctggaa ctgacagcga
    31501 cgttaaccgg gctggattac gggcgcttca cccgttcgat gctgctttcg caggggcaat
    31561 ttgctgcctt cctgaatgcc aaacccaaag aacgcgcgga attgctcgag gagttaaccg
    31621 gcactgaaat ctacgggcaa atctcggcga tggtttttga gcagcacaaa tcggcccgca
    31681 cagagctgga gaagctgcaa gcgcaggcca gcggcgtcgc gttgctcacg ccggaacaag
    31741 tgcaatcgct gacagcgagt ttgcaggtac ttactgacga agaaaaacag ttacttaccg
    31801 cgcagcagca agaacaacaa tcactaaact ggttaacgcg tctggacgaa ttgcagcaag
    31861 aagccagccg ccgtcagcag gccttgcaac aggcgttagc cgaagaagaa aaagcgcaac
    31921 ctcaactggc ggcgctcagt ctggcacaac cggcacgaaa tcttcgtccg cactgggaac
    31981 gcatcgcaga acacagcgcg gcgctggcgc atactcgcca gcagattgaa gaagtaaata
    32041 ctcgcttaca gagcacaatg gcgcttcgcg cgagcattcg ccaccacgcg gcgaatcagt
    32101 cagcagaatt acagcagcag caacaaagcc tgaatacctg gttacaggaa cacgaccgct
    32161 tccgtcagtg gaacaacgaa ctggcgggtt ggcgtgcgca gttctcccaa caaaccagcg
    32221 atcgcgagca tctgcggcaa tggcagcaac agttaaccca tgctgagcaa aaacttaata
    32281 cgcttgcggc gatcacgttg acgttaaccg ccgatgaagt tgctagcgcc ctggcgcaac
    32341 atgccgagca acgcccactg cgtcagcgcc tggtcgcgct gcatgggcag attgttcccc
    32401 aacaaaaacg tctggcgcag ttacaggtcg ctatccagaa tgtcacgcaa gaacagacgc
    32461 aacgtaacgc cgcacttaac gaaatgcgcc agcgttataa agaaaagacg cagcaacttg
    32521 ccgatgtgaa aaccatttgc gagcaggaag cgcgcatcaa aacgctggaa gctcaacgtg
    32581 cacagttaca ggcgggtcag ccttgtccac tttgtggttc caccagccac ccggcggtcg
    32641 aggcgtatca ggcgctggag cctggcgtta atcagtctcg attactggcg ctggaaaacg
    32701 aagttaaaaa gctcggcgaa gaaggtgcgg cgctgcgcgg gcaactggat gcattaacga
    32761 agcagcttca gcgcgatgaa aacgaagcgc aaagcctccg acaagatgag caagcactta
    32821 ctcaacaatg gcaagccgtc acggccagcc tcaatatcac cttgcagcca caggacgata
    32881 ttcaaccgtg gcttgatgca caagatgagc acgaacgcca gctgcggtta ctcagccaac
    32941 gacatgaatt acaagggcag attgccgcgc ataatcagca aattatccag tatcaacagc
    33001 aaattgaaca acgccagcag cagctcttaa cggcattggc gggttatgca ctgacattgc
    33061 cacaggaaga tgaagaagag agctggttgg cgacacgtca gcaagaagcg cagagctggc
    33121 agcaacgcca gaacgaatta accgcgctgc aaaaccgtat tcagcagctg acgccgattc
    33181 tggaaacgtt gccgcaaagt gatgatctcc cgcacagcga agaaactgtg gcgctggata
    33241 actggcggca ggttcatgaa caatgtctcg cattacacag ccagcagcag acgttacagc
    33301 aacaggatgt tctggcggcg caaagtctgc aaaaagccca ggcgcagttt gacaccgcgc
    33361 tacaggccag cgtctttgac gatcagcagg cgttccttgc ggcgctaatg gatgaacaaa
    33421 cactaacgca gctggaacag ctcaagcaga atctggaaaa ccagcgccgt caggcgcaaa
    33481 cgctggtcac tcagacagca gaaacgctgg cacagcatca acaacaccga cctgacgggt
    33541 tggctctcac tgtgacggtg gagcagattc agcaagagtt agcgcaaact caccaaaagt
    33601 tgcgtgaaaa caccacgagt caaggcgaga ttcgccagca gctgaagcag gatgcagata
    33661 accgtcagca acaacaaacc ttaatgcagc aaattgctca aatgacgcag caggttgagg
    33721 actggggata tctgaattcg ctaataggtt ccaaagaggg cgataaattc cgcaagtttg
    33781 cccaggggct gacgctggat aatttagtcc atctcgctaa tcagcaactt acccggctgc
    33841 acgggcgcta tctgttacag cgcaaagcca gcgaggcgct ggaagtcgag gttgttgata
    33901 cctggcaggc agatgcggta cgcgataccc gtaccctttc cggcggcgaa agtttcctcg
    33961 ttagtctggc gctggcgctg gcgctttcgg atctggtcag ccataaaaca cgtattgact
    34021 cgctgttcct tgatgaaggt tttggcacgc tggatagcga aacgctggat accgcccttg
    34081 atgcgctgga tgccctgaac gccagtggca aaaccatcgg tgtgattagc cacgtagaag
    34141 cgatgaaaga gcgtattccg gtgcagatca aagtgaaaaa gatcaacggc ctaggctaca
    34201 gcaaactgga aagtacgttt gcagtgaaat aactattcag caggataatg aatacagagg
    34261 ggcgaattat ctcttggcct tgctggtcgt tatcctgcaa gctatcactt tattggctac
    34321 ggtgattggt agccgttctg gtagttgtga tggtggtatg aaaaaagtca ttttatcttt
    34381 ggctctgggc acgtttggtt tggggatggc cgaatttggc attatgggcg tgctcacgga
    34441 gctggcgcat aacgtaggaa tttcgattcc tgctgccggg catatgatct cgtattatgc
    34501 gctgggagtg gtggtcggtg cgccaatcat cgcactcttt tccagccgct actcactcaa
    34561 acatatcttg ttgtttctgg tggcgttgtg cgtcattggc aacgccatgt tcacgctctc
    34621 ttcgtcttac ctgatgcttg ccattggtcg cctggtttcc ggctttccgc atggcgcatt
    34681 ttttggcgtc ggcgcgatcg tgttatcaaa aattatcaaa cccggaaaag tcaccgccgc
    34741 cgtggcgggg atggtttccg ggatgacagt cgccaatttg ctgggcattc cgctgggaac
    34801 gtatttaagt caggaattta gctggcgtta caccttttta ttgatcgctg tttttaatat
    34861 tgcggtgatg gcatcggtct atttttgggt gccggatatt cgcgacgagg cgaaaggaaa
    34921 gctgcgcgaa caatttcact ttttacgcag cccggccccg tggttaattt tcgccgccac
    34981 gatgtttggc aacgcaggtg tgtttgcctg gttcagctac gtaaagccat acatgatgtt
    35041 tatttccggt ttttcggaaa cggcgatgac ctttattatg atgttagttg ggctagggat
    35101 ggtgttggga aatatgctaa gtggcaggat ttcaggacgt tattcaccac tgcgcattgc
    35161 agcagtgact gactttataa ttgtactggc actgctgatg ctctttttct gcggcggcat
    35221 gaaaacaacg tcgcttattt ttgcttttat ttgttgcgcg ggattatttg ccctttcagc
    35281 acctctgcaa atattattac tacaaaatgc caaaggcgga gagttattag gtgccgcagg
    35341 tgggcaaata gcgtttaacc ttggtagcgc cgtcggcgca tattgcggtg gtatgatgct
    35401 gacgctgggg ctggcatata attacgtggc gctgcctgcc gccctgcttt cgtttgctgc
    35461 gatgtcgtca ttgctgctgt atggtcgcta taagcgccag caagcggcgg atagtccggt
    35521 gctggcgaaa ccactggggt agattgcagt cacgttgcgc gttgcttatt gccggatgcg
    35581 gcgtgaacgc cttatccgcc ctacgcggtt ccggcacatt ttgtaggcct gataagacgc
    35641 ggcaagcgtc gcatcaagca tgatgcgcca attgcctacg ttttttactc ttgtggccat
    35701 aaccacgcag cgccgcgtac gccgctggaa tcaccgtgct tcgccttacg caccggcgtt
    35761 tcacattcgc cgccgaagac aaattgttta atcaactgcc caaccgtttg atataaacgg
    35821 tctacattgc tcatcccgcc ccccaggaca atcacatccg gatcgagaat attcacgaca
    35881 tgtgccagcg attttgccag ccgcagctcg tagcgacgca atgccagttc cgctaccgga
    35941 tcgctttctt caaccaggcg gataatttca ctgcctttca gcgcatgtcc gctcaaacga
    36001 cgataatcag tcgcgaatcc cgtgcccgaa ataaaggttt caatacaacc ttgtttaccg
    36061 caataacaag ggacttcctc gcgataacgc agttcgtctt cgtccatcca cggtagcgga
    36121 ttgtgtcccc actcacctgc gtgccattgc cgccgatatg cgcccgccca ttgaatgcca
    36181 cgcccgcgcc gcatcccgtg ccgataatca cggcaaatac tgtctgcgct cccgctgccg
    36241 cgccatctac tgcttctgaa accgccagac agttggcgtc atttgccagc cgcacttccc
    36301 gctgcaacct cgcgcttaag tctttatcga atggctgacc gttgagccag gttgaattgg
    36361 cattcttcac cacaccggtg taaggcgaaa ttgagccagg aatgcccata cctaccgttc
    36421 cgcgctgccc tgtcgcctgc tccgccatat caaccaacgt ggcgatcgtt tcaatagttt
    36481 gccggtaatc atcacgcggc gtgggcagac gatggcggta caactgctcc cctgcatcgc
    36541 ccagtgcaat cacttcagtt ttggtgccgc ctaaatcgat acctatacgc acggtactct
    36601 ccttattttt ttcaatatca atagcgtaga gacggacaac cggattggca atgcaaggcc
    36661 gccgacaatt cgttatcatg cccgctaaat ttaacgacaa ggccgtggaa attatcatgc
    36721 tgtggttcaa aaatttaatg gtttaccgtc ttagccgcga gatttcgctg cgtgcagaag
    36781 agatggaaaa acagctagcc tcgatggcat ttaccccatg cggcagccag gacatggcga
    36841 agatgggctg ggttcctccg atgggatcgc acagcgatgc gttaacgcac gttgccaatg
    36901 gtcaaattgt tatctgcgcg cgcaaagaag aaaaaatcct cccgtctccg gtgattaaac
    36961 aggcgcttga agcgaaaatc gccaaactgg aagcggaaca ggcgcgtaag ctgaagaaaa
    37021 ccgaaaaaga ttcgctgaaa gacgaagtgc tgcactctct gctgccgcgt gctttcagcc
    37081 gttttagcca gacaatgatg tggatcgaca cggttaacgg tttgattatg gtggactgcg
    37141 ccagtgccaa aaaagcggaa gatacgctgg cattactgcg taaaagcctg gggtcgttac
    37201 cggttgtacc attgagcatg gaaaacccga ttgaactgac gctgaccgaa tgggttcgct
    37261 ccggtagtgc ggcacagggc ttccagctgc ttgatgaagc cgagctgaaa tcgttgctgg
    37321 aagatggcgg cgtgatccgc gcgaagaaac aagatctgac cagcgaagag atcaccaatc
    37381 acattgaagc cggaaaagtg gtgactaaac tggcgctcga ctggcagcag cgcattcagt
    37441 ttgtgatgtg cgacgatggt tcgcttaagc gtctgaagtt ctgcgacgag ctgcgcgatc
    37501 aaaacgaaga tatcgaccgt gaagatttcg cccagcgttt tgatgccgat ttcatcctga
    37561 tgactggtga actggcagcg ttaattcaaa acctgattga aggattaggt ggcgaagcac
    37621 aacgttaatt gctgattttc ctttaatgcc ggatgcgacg cctgccgcgt cttatccggc
    37681 gtacgaagcc acaccaggca tataattatt cgctacggcg ggcaataatt tttagcgcag
    37741 caatattatg cgttttatgc tgtaactgat ctaatcgcct catccctatt cgcctgtcag
    37801 cagcacgcag aatattcact aacccccatt tatcgctggt aaattgtttt gttaacaact
    37861 ccgcttttgg ggtgtcaata tattcacgca ataaatcaga aattgaacaa atatccgtca
    37921 ggtaaggata ccagcaagtt tcaccgtgaa aattacgcac tccaccgtta cctgcaataa
    37981 agtctttcgg gtaatcccag atcacgtttt tatccaacgt gatccaataa cgcggtaaat
    38041 cggtgcttcc accattttgg ctgcgcatgg gataacgggt acagtgaatt tgcaggttga
    38101 tcgttggcgt cagtaagtca tagatctccc gctgtaactt gctccagggg gcgttgtgtc
    38161 atcagttccg caaaaaatac ctggagatct tcactgatat ttatatcaac cttgatgaat
    38221 tatgaatata tcggaaattg aaaaaatcga agcaaaggaa atattattat gggttaaaga
    38281 gaggatattt cagaaaaatg ccctcccgtt caaggggaag gcgaggaatt acagatagcg
    38341 gcacagataa gaggtgggtt cggcaacttg cagatgaaac tcactgtgac cgggaacatt
    38401 aaacaccgaa ccggcttcat acacttgcca gtcggtcgcg tcaggcagta acacattcag
    38461 cgcgccactg attaccgtca tctcttccgg ctcagcggtg ctgaaggtgt attcgccttc
    38521 aaccataaca cccacgctgg cgcgaccagt gctgctgctg gaaaagccga ttgatttcac
    38581 tttgccggaa aagtactcat tactttgaag cataaactgg ccctgtttgg tagttgaaca
    38641 tctacttcac tataggggcc agaggcgctg actgtcacgc aaaattacac cagtaattcc
    38701 gcagccagcc gtgcaatcaa tacgttagac agcaagacag gaacatcgag ctgtttttgc
    38761 agtaaatcgc gatgacgttg gtgaaatcct aaacaatcca gcatgatgac atcagcccct
    38821 tttgccagta attctttccc ggcatcaatg attttttgtt ctgaatcatg aatggggtta
    38881 cccaatgaaa ataccggcga tttctgcaaa atttgccatt tttgcgcctg aacgggcagc
    38941 atctcctcaa ccggaacgat aacccccacc tgatgatctt caacaataga ggaaaccagt
    39001 ggaggcaata ttcgcgacgg ctcaagaaag atcgtattac gcgcagtcat actactaatg
    39061 tttgctgtac tcattaataa aatgacgtca taaccctgat tatcgagcac ttcaaccaca
    39121 ccttgcaggt ctcgctccac tttacgacgc gaaacatggg ccagctggtt gtcatttaat
    39181 aatgtgagaa tggtgtcttc gcctgcttct ggcgcgtact ccgccatcac ttcttcacga
    39241 cttaacttcc ccagcaggct atgatgggaa atattatctt cgtcaatgta ttccgtcagg
    39301 agcggcaaaa cctcctgcat aggtacaatg ccgatggtca ggatcgccaa cgacgcactc
    39361 attgttcatc caccttactt ttctttcgtc gttaattacc gggcaagtgt gaagcaccac
    39421 tgctgacact tacttgtgct acaaatgaca aaaagcgtag cagcagacgt gcacggcata
    39481 atgtaaagat tccaaatgat tccagtaatg gattgttatt gtttaatatt ctaattatta
    39541 gaaaaacatg aattatgaaa aatgtgacgc agatcccttg tagtggcact cagaatccct
    39601 tccgagcagt ctgatgcggt cactctttgt cagggtcctc atagcgtttt ttcgcatcca
    39661 tcgcttcctg agccgtcgga tgttcactga tcaacgagtc tggtttagga tgatcggctc
    39721 tgagttggta ccaggttacc gtctgtcctg gctttccttt ttcaatcgtc actatatatg
    39781 cttcacgagg ataaggcggt ttcgttggca tcgttcttcc ttatttcacg ggatgaacgt
    39841 taagtatagg cgctctaaaa tcaacaattg atcgtctgtg ccagggcgct acgaatttca
    39901 gaaatcacct ggctgggttc gtttgttgcg tcgatgataa tatgcgcaac ttcgcgatat
    39961 agcgcatcgc gttcttccag cacttcctga acttcttcgc tcagcggttt tcccgttaag
    40021 gttggccgta aatcttcttc cggtgcagct tgcagtcggt taaccaggac tgatactggc
    40081 gcacacaaat aaaccacgat cccgttattt tgcataaagt gacgattaaa ttccgtcaga
    40141 ataatgccgc cgcctgtagc gataacggtg gatggcgcag ttaccgcttc cagcgccgcc
    40201 gtttctctgg cgcgaaatcc cgcccactct tccctttcga cgatctccgc gaccgtcata
    40261 ttgagctgtg attgcaacca ctgatcggta tcgacaaacc gacggttaag cgaatcggca
    40321 agggccattc cgaccgttgt tttaccacag ccccgaggcc cgatcagaaa aagaggttgt
    40381 gtcatcgtgg gttttcccca ataggtcgca atgcggcgaa agccggtgtc atgagaatag
    40441 cgatcatacc atcaaactag tacaatttcg attgtaaaga aaaaattcca cttaaagtga
    40501 aaatctcaat acatccctta ctataccaat aaatattcaa gaatgaagtg taaataataa
    40561 attacattta gccacgacta cgttgcactt ccagccacca cttctccagc tccgcggcaa
    40621 aggcctggcg gtcacgttgt gaaaggctat ctggtccgcc ggtctggatc ccactggcac
    40681 gtaaggtatc cataaaatcg cgcatcgtca ggcgctcacg aatggtcgct ggcgtgtaac
    40741 gttcgccgcg cggattaagc gccgctgcgc ctttctcgat ggcttcagca gccaaaggta
    40801 tatctgcggt gatcaccaaa tcgcccgctt cacactgccg gacaatttcg ttatcggcaa
    40861 cgtcgaaacc tgccgcgacg cgcagcgtac gaataaatcg cgatggcggc acgcgtaaac
    40921 tctggtttgc taccagtacc agcggcatct gcatacgttc cgccgcgcga tacaaaatct
    40981 ctttaattac attgggacac gcgtcggcat ccacccaaat tgtcataaag tcatcctttg
    41041 ttgggtaatc ctctattgtg tcgcgctttt gccttccggc atagttctgt ttatgcttct
    41101 gccagcgatt atcaaaacaa tgaatttcac ggcaggagtg aggcaatgga aaagaaaatc
    41161 ggttttattg gctgcggcaa tatgggaaaa gccattctcg gcggtctgat tgccagcggt
    41221 caggtgcttc cagggcaaat ctgggtatac accccctccc cggataaagt cgccgccctg
    41281 catgaccagt tcggcatcaa cgccgcagaa tcggcgcaag aagtggcgca aatcgccgac
    41341 atcatttttg ccgccgttaa acctggcatc atgattaaag tgcttagcga aatcacctcc
    41401 agcctgaata aagactctct ggtcgtttct attgctgcag gtgtcacgct cgaccagctt
    41461 gcccgcgcgc tgggccatga ccggaaaatt attcgcgcca tgccgaacac tcccgcgctg
    41521 gttaatgccg ggatgacctc cgtaacgcca aacgcgctgg taaccccaga agataccgct
    41581 gatgtgctga atattttccg ctgctttggc gaagcggaag taattgctga gccgatgatc
    41641 cacccggtgg tcggtgtgag cggttcttcg ccagcctacg tatttatgtt tatcgaagcg
    41701 atggccgacg ccgccgtgct gggcgggatg ccacgcgccc aggcgtataa atttgccgct
    41761 caggcggtaa tgggttccgc aaaaatggtg ctggaaacgg gagaacatcc gggggcactg
    41821 aaagatatgg tctgctcacc gggaggcacc accattgaag cggtacgcgt actggaagag
    41881 aaaggcttcc gtgccgcagt gatcgaagcg atgacgaagt gtatggaaaa atcagaaaaa
    41941 ctcagcaaat cctgatgact ttcgccggac gtcaggccgc cacttcggtg cggttacgtc
    42001 cggctttctt tgctttgtaa agcgccaaat ctgccgattt caaccactca cgatagtgac
    42061 tcatttgtgg gttcagcggc gcaaccccca cactaatccg taaagttacc tgtggcgtat
    42121 tcggcaagcg taacgtgttt agcccttcac gcacccgtaa catggcggta atggcgctct
    42181 cagctggcgt accggacatg attactgcaa actcatcgcc gccaaaccga ccaatcacat
    42241 cgctaccgcg cagggtaatt tgtaactgtc gggtaagcgc cacaatcgct tcatcgccca
    42301 catcatgacc ccaggtatcg ttgatgctct tgaaatggtc gatatcgata atcagtaacg
    42361 ttgcatcgcg attatgccgc cgacagttat caaattcatt gcgtaacata gtttcccaat
    42421 gacgtcggtt atacacgccc gtcatgccgt cgcgggtact catgacctgc aacctgcgtt
    42481 tatgttccgc cagtttggtt gccgtctggt agctgaccca gccaaacagc agaggataaa
    42541 cgacaataat gggaagggag agccaccatt ccagcggcgc actattgaac gacacggtaa
    42601 tgcccgtaag ctcgagggtg acaaggcagg aaaccaccat caacaccaga cccgcgacaa
    42661 acagacgggg gccgcctgcc cccatcaaat tcagacacat aatcatcaac atagcggtgg
    42721 aaggcagcac gtttacgccc attacgccta cccacattcc cgctaatact gcatcggttt
    42781 ttaagttgta aatttcccag ctaagcggat cgacggccct gctcgctatc tgccaggcta
    42841 aatgcggcca gacgaacgcc cagccgacca acaccagcca ccaccagccc ggcggcgggt
    42901 gcgaaaccag cgttgaagca atcggtaaga acatgccagc caggccaacc gcacggggta
    42961 gtctgacgcg acgggcgaag cgcagcccgg accgctgatg ttcgttttga ggagttaaag
    43021 gaggttcctc cccatgcgcc gccgcttttt tgaaaaagtt ttcatcattc attatttttg
    43081 ggaacattca gaaacaattt tcccaaatta tagagacggg gctaaaggca gaagtatgaa
    43141 atttcgggtg agccactttc gtagctcacc cgttagcact atcaggtatc aggcggcttt
    43201 cttgaggcag gcactcataa acttattacg atcatcacct ttcagagatt gttgcgttgc
    43261 ttgattatta cattcgcgca tcttttgctg ctgtggcgtc aaactttttt caccaggcgc
    43321 agacttgctg ttcttcaggc aatcactcat gtaggtctta cgagcatccc ctttcaacgc
    43381 ctgcgccgtc gcctgctgat tacaggatgt aatacgctgt tgttgtgggg ttaaggttct
    43441 ctcggcagcg ccgacggtgg ttaaaaaaac cagaccaaaa agcaaggtaa ccagtaatgt
    43501 tattttcata gcaccatccc tcttcatgtt ttaaccatga gcgtatgcgc ccgtgatctg
    43561 ccattaagtc tggttgctaa cagcaaaaaa accacccggc agcgaaaatt cactgccggg
    43621 cgcggtttta tttcagcccc agggcggctt tcatggtgta gaagagatcg gtctggtcgg
    43681 tcagtccaac aacattggcg gcatgcgggc catacgccgc aatacgcaac tgactgccgg
    43741 tatgttcttg tgaatcctct tcggagttcc cgtagctcat caccatcact gcgccatctt
    43801 tggtatttag cgcctgggtg aggcccggag ctttggtatc cggcgcgaca atctggctgg
    43861 cgtgggcgtg atcagcggtg actatgacca gcgtattgcc atccttttta gcgaattcca
    43921 gcgcccgttg tacggcttca tcgagatcga ccgtctcgcc aatttgccca caaggattcg
    43981 cagcgtgatc ctgtttatcg attgacgcac cttcaacttg caggaaaaat cctttctcat
    44041 ttttactcaa caattcaatg gctttgtcgg tcatctgcgc cagggttggt acactgtcat
    44101 tacgttgcgg attaggcgta caggtgactg cgggcttatc gatattgccg tggtacgttg
    44161 ctttcggtcc tagccagcgc actggcatat tgccgtcagc aaacagtcca agcagaggtt
    44221 tttgctgatt cgcttccgtc accgaattca gtgaggcagc atcgctgacc aactgataac
    44281 cacgcgcctg tgcctgttca cgcagcgttt ttccctgcca ttcaccggcg gttgccgttt
    44341 cagcaaaggt ttttgcgcca ccgccaagcg taacgtcggc acgagcgtta agcagctgtt
    44401 cggtaatcga tccttttccg cctttttcca gagcgttacc cggacatttt tcactggtcg
    44461 cgctcggacc gtagcatttg cgcgaagtca catgtgccac cagcgcagcg ggcgtggcat
    44521 cctgcaactc tgcggtagaa acgttaccgg tcgccagacc tgcggctttt gccatttcca
    44581 gaatcgttgg gtgatctttt tcgtgaatat cgacgcccag cgcgccgtta taggttttga
    44641 caccggttga ccaggcggtt gctgatgcag ccgaatcggt gacgtagtcc ggtttgccgg
    44701 tttttttatt cagcgcatag tgagtgtatt gcccggtaag cggtaaggca tcgatacctt
    44761 taaaaaagcc gcccgcacct tcggcataat tacgtgcggc tgtaatttcc gaatccccca
    44821 tcccatcgcc aatcagcaaa ataatatttt ttgcaggttt atcgctaaga gaatcacgca
    44881 gagcggcagt ctgatcaccc gttaaacggc gagcaccgcc gggtgcagta atatcgccct
    44941 gagcagcccg gttttccaga acaggcattt ctggtgtccg ggcttttgtc acaggggtaa
    45001 acagtaacga taagagtgcc agtgcaatag tgctttgttt cactttattt tctccatgta
    45061 caaatacatt aaaaaataaa aacaaagcga ctataagtct cggccgtgac aactttatga
    45121 cagctgttga aaagattaac tttttactga cgaggatgct tcaataactt ctttacgtaa
    45181 tcgcgcagca gctccgtatc gtcgtcagga atgctggcat cgggctttac ctcgtacagc
    45241 gccccctcta cctgatcaat caaccgctgt tggtcatttt gcgccatatt gcgaagcatt
    45301 gcagtgacga taatctccaa agcttctacc tgcgcacaca gttctttcga ctcttcttct
    45361 ttttgggcaa gcttaaataa caactcagca atgagatttt tcatgtctgt atttccttat
    45421 ccaaagtatg gagaagttaa cattatgttt cacaacttca tagtgtctgg aagtattatt
    45481 tttatgacaa aggaaatact ttgcgcaaat attttttgtt tgattaccag cgaaacgttt
    45541 ctcttattta aataactacc cacacaaaat ataggctttt aattatcaac ctttattata
    45601 aataaaagtt atgaaatata ttctcttcgt taacttataa ttaagagaaa aaaactcacc
    45661 gcatggccat ttaagccaga aataaattag cttcccgttc atcatttttg tgatcttgcg
    45721 cacatctttg agataacgca ctttcttcac cagtacgttg cacgctttgt cagcagaaga
    45781 ttgcaacaat ctttcagaag gccgcgaatc ccccgttttg tcgtggtaat gtatgcactt
    45841 tgtgttagac tggataggtt tttaagatgg aaaaactgcg ggtaggaatc gtttttggtg
    45901 gtaaatcagc ggaacatgaa gtgtctctgc aatcggcaaa aaacattgtc gatgccattg
    45961 ataaaagtcg cttcgacgtt gtgctgctgg gcattgataa acaagggcaa tggcacgtca
    46021 gcgatgccag caattatctg ctaaatgcag acgatcctgc ccatattgcg ttgcgccctt
    46081 cggcgaccag ccttgcgcag gtgccaggta aacatgagca tcagcttatc gacgcgcaaa
    46141 acggtcagcc gttgccgacg gtggatgtca ttttcccgat tgtccacggt acgctgggcg
    46201 aagatggttc cttgcaggga atgctgcggg tcgccaattt accgtttgta ggttctgatg
    46261 ttctggcttc agcagcctgt atggataaag atgtcaccaa acgtctgcta cgcgatgccg
    46321 ggctgaacat tgcgccattt attaccctga cgcgcgctaa tcgtcacaac atcagttttg
    46381 ccgaagtgga gtctaaactg gggttaccac tgtttgtaaa accggctaat cagggctctt
    46441 ctgttggtgt cagcaaagta accagtgaag aacagtacgc aattgccgtc gatctggcgt
    46501 tcgagttcga tcataaagtg atcgttgagc aagggatcaa aggtcgtgag atcgaatgcg
    46561 cagttctggg caacgacaat ccgcaagcca gcacctgtgg cgagatcgta ctcaccagcg
    46621 atttctatgc ctacgacacc aagtacattg acgaagatgg cgcgaaagtg gtagttccgg
    46681 cagccattgc accagagatc aacgataaga tccgggcgat tgccgttcag gcttatcaga
    46741 cgttgggatg cgcaggcatg gcgcgtgtag acgtgttttt aaccccagag aacgaagtgg
    46801 tgatcaacga gatcaacaca ctgcctggct tcaccaacat cagtatgtat ccgaagctgt
    46861 ggcaagccag cggtctcggt tacaccgatc tgatcacgcg tttgattgag ctggcgctgg
    46921 agcgtcacgc tgcggataac gcattgaaaa ccacaatgta atattttaac gcccgataat
    46981 attcttattg ggcgttttca gtttcttcat cgcgacgacg aataatcagc cccgccagcc
    47041 agaaactaat cacccacgtc accagaccga cggcataggt ctgccaacct tttgcttcaa
    47101 atcccagtaa ccccaccacg ccattaagaa tgaatataag gccaatggcg aacgcatagt
    47161 agtgccagtc acggcggatt tttacaggca gattcatggc agctccgtta acgaaaaaca
    47221 acatccttgc atattctgta acaaccgtct gtggtgtaga caacactttc gtaattgtta
    47281 ataaatttaa cttaaggttg ccattttgtg actcacaacg aagattcacg cttcgggaat
    47341 attcccaaca tcaatttacc atgattctga tattgacaat ctaaatgacc tgtcagactt
    47401 gccataagcc gctggtatca caccagaaga gaagcagatg acttccggag gtttttatgg
    47461 ctgatttcac cctgtcaaaa tcgctgttta gcggaaaata tcgcaatgcc tcttcaacgc
    47521 ctggcaacat tgcctatgcg ttgtttgtgc tgttttgctt ttgggctggg gcgcaattgc
    47581 tgaacctgtt agtgcatgcg cccggcgtct atgagcgttt aatgcaggtc caggaaacag
    47641 gtcgcccacg ggtggaaatt ggtttaggtg tcggcaccat tttcgggctg atcccgtttt
    47701 tagtaggctg cctcattttt gcagtggtgg cgctatggct gcactggcga catcgccgcc
    47761 agtaagattt acgttgccag atacggccta aaacgcctta tctggcatga cattcctgtt
    47821 tgatcactgt ttcatacaac gcgcccgccg ttcgtctacg cgcttcgcaa accaggccgt
    47881 agtcaggttg cgggtgattt tcgggctttc cagttgaatg ccaggcaaca tttcgcgggg
    47941 tagtgattta cccgttttcg cttcggcaag ttgataaact ttcttgtaca gcgccgtctc
    48001 ctcaaaagag aagctgtcac ctttctctaa ctgacggcga atttcgctgt cgctcatccc
    48061 cagttttgcc gccagtttgc gcgttgccag ttcggttttc ccgggttctt tactgtcgta
    48121 acgaattaaa tcaccgtcca gtgccagctt cacgccgctg gccttactga ccgcgttctg
    48181 aaacgcggca ttacggctgg cgtaccagcc agcgttaaaa tcagcaaagc ggtatatcgg
    48241 tgcgctatag ctggcgggat aattcagtaa atggtaagta ccaaaccaca acccgccgcg
    48301 gcgactgaag acttcctgac gaactgtacc gtccattttc cacggatacc ctttggtatg
    48361 ctgttcggca aaagcaatgc tgacctgcat cggcccaccg gtgcgcacag gattaagcga
    48421 accaaacagc gtctgcccca ttggtaccat gctgatcaga tcgtcaaaaa tcgcgctcaa
    48481 ttgcttttct gtacgcaccg aatctaaccg ttcgctataa cttttgccgt ttggcgattt
    48541 gatttttagc gcggtatgaa ccagaaaagc aggaatgtgc atccgttcag cacgacggtc
    48601 aatctcttgc caggcgatct tgcttaagcc cggaacggcc ggatctgcct gataatttga
    48661 ctcctgctgc gccaccgcca gcaccgagca gacgttttcc agcgtcggtg ccagcccctg
    48721 actttcaaag gtggtggcga gatctttcgc ccaggcatcg cggtctttca cgctcgcagg
    48781 catcttctgc cgcacgacgc tcgctacatc tatcgctttt tcgccctttt tcaacggttg
    48841 cggagcctga ctactacacc ccgctaaaac cagaaccgcc aacaatgaca gcgaagatag
    48901 atgattgacg cgcgacatta cttctccttt ttagctcaag gtatgggtta cttcctgaat
    48961 tttgtcacca tccagctcat gttcaaagct gcgcagacgt ttgtagatag acatcaactc
    49021 aaccagtgtg gtccatgagt taatcaggta ctggaaagca ccgcgaacct gaccaaaaac
    49081 gttggtaatc tgcgtcatca ggccgagcgt aatcgtaccg gcaacaattg acggaaacag
    49141 caagaacaaa ccgaaaacgt tatcgacctg caaatagaga atgcgggcga tgttgaaata
    49201 catatagtga aaatagaggc gaaaatagtt tttccgtacg gcgctaaaca gctcgcgtac
    49261 cgtaggcggc gtcgcgcgcg tggcatcgtc ttcaccataa accagctctt tacggtaggc
    49321 ggcctctact cgctggtttt taaactccag ccccggcagt ttgatcccta ccactgccag
    49381 caatccggtc cccatcagcg accagacgat agcggcaatc accagaccat acggaatgtg
    49441 cccgataatc ggcagctccg gcacatgcgc ggagagcgtt accagcaccg gcagaaaggc
    49501 gatcaacgtc atgatggcgt tgataaaact gacgcccata ttctccagcg ttgaagcaaa
    49561 acgcatggtg tcttcctgca cacgctgtgc ggcaccttcg atatgacgca gttgttgcca
    49621 gttcgccatg taatattcgt tcatcgccgt acgccagcgg aacacatagt gactgacaaa
    49681 gaagttgttc agtacactga tcaccaccgc gatcagcgca atccccagaa agacgcccac
    49741 ttcgcggtaa aattgttcga tagtgacttt atgcggcgaa cttagcgcgg tttgaatcag
    49801 atcatagaac ggtgcatacc aggcgttgac ggcgaccccg acttccacca aaaaccaggt
    49861 gacgaagatg atcagtgcag taccgagtat tgaccagtat tgccaacggt gcgggctgta
    49921 gataaaccag aaaaatgcaa aaagtcctac gcaaacaatg tagtaagcgt aaaaaatcag
    49981 gaaatccaac gaccagaaac gcgcggcgct aatcgggatc tgcccggaag cgccggtgat
    50041 acgcgccacc cagtcacccc caccggcttg ccagaagata acggcaatca atgcccaaac
    50101 aaaggccgag agaaaaaacg ctcccggctt tgggaaaaag acttaaacat atgcactcct
    50161 gctaacttct tatcgtttta acaactgctg tgtattaacc gcatgaccac tacaaagcgt
    50221 cagccaaatg aaaccgccca ttgtgaccca tagcaaggcg cttagttcgc cagcctgacc
    50281 ataagaattg tttcgacgga tttcactaac caaagattgt ttacataagc ggattaacac
    50341 gcgcaggatc acaaatgcag gcattggtga gcaggaaaaa gatcgtgttt acgcgccgtt
    50401 acgtttttaa cactattatt gcactaattc gccctttgca atccatcaat gagtagtata
    50461 aatacgctca gttaccttca ttcaatctat ggacaccacc gttgaaacgt agtctgcttt
    50521 tttctgccgt gctgtgtgcg gcgtcattga cctccgtcca tgcggcgcag ccgatcactg
    50581 aaccggagtt tgcctctgat attgtcgatc gttatgccga tcatattttt tacggcagcg
    50641 gtgccacggg gatggcgctg gttgttatcg acggtaacca gcgcgtcttt cgtagttatg
    50701 gcgaaacgcg acctggtaat aacgttcgcc cgcagctgga ttcggtcgtg cgtattgctt
    50761 ccctcaccaa actaatgacc agtgaaatgc tggtgaaatt gctcgaccag gggaccgtaa
    50821 agttaaacga tccgctaagt aaatatgctc cgccaggcgc acgcgtgccg acctacaatg
    50881 ggacgcccat taccctagtg aatctggcaa cccataccag cgccctgccc cgtgaacagc
    50941 ccggtggcgc ggcacatcgt ccggtgtttg tctggccaac gcgcgaacaa cgctggaaat
    51001 acctttctac ggcgaagcta aaagccgcgc caggcagtca ggcggcgtac tctaaccttg
    51061 cgttcgacct gctggcagat gcactggcga atgcgtccgg caagccttat acccagttgt
    51121 ttgaagagca gattacccgt ccattgggga tgaaagacac cacctacacc ccctcaccgg
    51181 atcagtgccg ccgtttaatg gttgctgagc gcggtgccag tccgtgcaat aacacgctgg
    51241 cggcaattgg cagcggcggc gtctattcca cgcctggcga tatgatgcgc tggatgcagc
    51301 agtatttgtc atctgatttt tatcagcgca gtaaccaggc agatcgcatg cagacgttga
    51361 tttatcagcg cgcgcagttt acgaaagtga ttggcatgga tgtccccggc aaagcggatg
    51421 cgcttggttt aggctgggta tatatggccc cgaaagaggg tcgtccgggg attattcaga
    51481 agacaggagg tggtggcggt ttcattacct atatggcgat gatcccgcag aagaatattg
    51541 gcgcgtttgt ggtggtcact cgttcgccgt tgacgcgctt taaaaatatg agcgatggca
    51601 tcaatgatct ggtgaccgag ctaagcggga ataaaccgtt ggttatcccc gcatcctgat
    51661 taatactctg aaggcaaacg gttgatagcg accagtttgc ctttattcat ttcgatatag
    51721 ccgcctttac gcaaagcagc gagaacttct gcgacgacag aacgcgatat gcgtgtacgt
    51781 tgatggatat aattcatcac accaatacgt gagcgcaatt cttcattcca gtcaatcatt
    51841 gataataatg ttgcgcggat ttgttcgtag gaattatgcc caatgagctg cacgtcgcgt
    51901 aattccagaa ttctattttg ccaggctaac cagtaaaaag cgtctcgcca gagttgattt
    51961 tgttcaataa gcgtaatggt ttgtttggct ggtagatgat atcccgtaca atttccttct
    52021 gatattaatt tgtatggtat atcgtttttc attaaaccat cagccagccc cataatataa
    52081 ggagcctggg taataccgat aagtacgttt tcttctctac gcagagagat aacgccctct
    52141 aaaataacga aagtatcttc gctattgaca tcactggaaa atataacttg cttttcatta
    52201 ttaaactcga agcgcgtacc gtatctggac aaacatttat cgagcttacc aaattcctga
    52261 agaggtttaa ctacagataa catttgcgcg tcctttgcag taatgcccgt caaatccttg
    52321 acgggcatta tttagattaa attaccagaa tttcttcgga gtgaagaata ttaccaggta
    52381 tatttaacac ccacgttcgc ggaccagtct tgatctacgt caccaccacc gaggtagtta
    52441 gcatcggtat aggcgctgaa gttcttggtg aagctgaact gagtacccag accaacacgt
    52501 accgcagacc cttcagtacc gttatcgatg gaatcaccgt tcacatcgtt atcgttgtta
    52561 gagtcgtcgt agacgtaagc cagtttgaag tacggagtca gagcctggtc ttcgctgtag
    52621 gtgaaggtat aacctgcatc tacacccagt tcataacgca tgctgtcgta agactgaccg
    52681 tcaactttca tgtcgttgct cagctggtag tcatcaccag actggaacag accagaaatg
    52741 ctgccgtaag gagtcacgta accagcatca cccagtttga agtcgtaacc ggctttcaaa
    52801 ccgaagcccc aagcgtcgga gttggtgcta ccgtcaacgt aagtaccgtt gctcatggtt
    52861 gcagacaggt cgttgttgaa gtgagagtaa ctcaagctac catcaacaaa gacgttgttc
    52921 gcgaagtgag cagaagagta gatgtaggca gtctggctgt cttgatccac ctgaccagaa
    52981 cggtcattca tgtcaccttt agcgaagcct gcagccgcac cgacgatcca cttagcgttg
    53041 ttatcgtcaa ttttggtatc aacaccgacc atgatgccgt taacatcctg atcatagttg
    53101 atggtgccgt tgtcgccgtt gaagttacca ccgaagtagc ttacccatgc gccgccgtta
    53161 tcagccaggc catgacgaga gttggtcaga cgagtaccaa cggtgtcttg ttccaggttc
    53221 cagatattgg tgttcgcaga cgggatgctc agcgccatgt tagcgtagtc ggtcagctcc
    53281 atctgttgca gaacaacggt gttaccgcgc tgttcagcct gataggtgta tgcacccagg
    53341 tcagctttgt tagcagcaga gaaggtcgcg ttgctgtttt tgtcgttgac gtagatcagc
    53401 tcgttgccgt tgtagtcagc gatagaacca gcaccagtag cgttgtcgat acgaactttg
    53461 tagttaccgg ttgcagctgc aacatggtca ccgttgttat caacttcgtt ctgaccagct
    53521 tcgatagaag cagtgttgtc gttatcgccg ttaccgttaa tggtcaggtg accatcagag
    53581 ttcattgcga ttaagccata accgtagttt gacttggtag tatcgttggt acggtcgttg
    53641 gtcaggtctg catcaagtac gaagcggttg ctttcgatgt tgaacacacc agcgtgaatg
    53701 ttaccactgg tatcagtagt gctggtcagg ttcagagtat caacctgcag agcattcgcc
    53761 gcagtgttac cgacatccag aataccgtca ttagtcactt tgatgttgtt ggcatacaga
    53821 gcagcatcac ctacagccca accagcagaa acttcatcag aaatggttac ggtactgttg
    53881 tcgacagtca gtgaatcagt tgcaacatga ccatcttcgt tgatgttcag cgcagaaaga
    53941 cctgtcagag agatagagtc agaaatcagc gttgaatcag caacattaac agttgaacca
    54001 ctgttgatgc tcagagtatc aatcagagaa gttttggtgg tatcccactg tgaaccggta
    54061 ttcagggtca cattgaacag accgctctgg tatacttcgt taccgatgat gtgatcaaat
    54121 tcgctgtatt cagagtcgct gttttctaca ccaacagttg atgaaggcca caggctgtta
    54181 gctgcgatgt cgatcagagt tgcagttgct tcggtgcctg tactggtatc aataacaacc
    54241 ccgttgtcat tgtaaacagg accgtaagca tctggcacac catcatcatt ggtatcaaca
    54301 gttaagtgcg cagacattgc cgcaccaacc cacttgctgc cattgttcag ggtcagatcc
    54361 aggcgatcag taccatccca accgttagta tctacatcac cgtcagcatc acgatagcta
    54421 tcacgaccat gcgggaagaa gttctcatca aagttacttg agaagaatac atcgcccatc
    54481 agtgtagaat tatcgaatgt tgcagtggtt tgcattgcgt tatcagcagc aggatgtgca
    54541 ataacggcta atgcgatatc atctctattc cagtcaccgc cattgtaatc gctggcattg
    54601 ccggtattac caaaccaacc actagtacct tcatcagacc atgaaccaga ggtcacagta
    54661 gagtctttaa cagtgatggt gttgttaaac acttcgctgc tgttaacacc agtgctaacc
    54721 gtgttgtcgt cgatatcttc ccactcataa ccctgagtca acgtaatacc cgcaacgtga
    54781 gagttattag tgatggacag atcaacttcc tgatccagag taatagccgt accaatgttg
    54841 taaacgtcta caacatgagt atcagcggca ttattgtaag tgccgttata tgtgtagtgt
    54901 tcgtagttgt cgtcgatcgt tgaattatca acagtcagcg ccagacggtc atggtaataa
    54961 tcattcgcac gttcagcaca atcagttgtc atgcactcgg aataaatcat accgtgaata
    55021 gtgctgtttt tgattgtcag actattggcg ttggtattgg tagaaatacc gtcgtccaga
    55081 tagtaggtag aaatcacgcc gttaacagta gcgttattaa tgaccgggta gatatcgccg
    55141 ttgtaataac tatcagcggc atagttgttc cagcctacat aaccatcata gtaaacgtca
    55201 tcagcatagg tggcgtcgtt gtagtgatgg aaagtattgt aagtttgacc agaaatatcg
    55261 ttggtcgctg cattagcctg agaggtgata gccagagtgc aagccaatgc taattgtgat
    55321 actacaagtt tctttttcca ggagtgcatt tgtcatccct cctcagggac gtaataagtt
    55381 gatccatcaa ttatcaatgc atagaaaaca tattatgttt cgcgctaacg attatgctgc
    55441 gttacagacg aatggttcaa ttattctttc ccctaagtcc gaatgtggac atatataatt
    55501 tatggagaaa aacacgcatc aaattgataa aaggacgata tttcaacatg ttgcagataa
    55561 tgttattttt gatacatacg cgttataaat a
//
LOCUS       DAZGSP010000021        53564 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZGSP010000021 DAZGSP010000000
VERSION     DAZGSP010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.021"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..527
                     /gene="yehD"
                     /locus_tag="ACHK74_001408"
     CDS             <1..527
                     /gene="yehD"
                     /locus_tag="ACHK74_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0220261.1"
                     /translation="AAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSV
                     SINMPTVPTTVFEGKAKYSTYDDAVGVTSRMLKISCPKEVAGVKLSLITNDKITGNDK
                     AIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            607..1281
                     /gene="yehC"
                     /locus_tag="ACHK74_001409"
     CDS             607..1281
                     /gene="yehC"
                     /locus_tag="ACHK74_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0220262.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            1297..3777
                     /gene="yehB"
                     /locus_tag="ACHK74_001410"
     CDS             1297..3777
                     /gene="yehB"
                     /locus_tag="ACHK74_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP0220263.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEEAFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAIADLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            3791..4825
                     /gene="yehA"
                     /locus_tag="ACHK74_001411"
     CDS             3791..4825
                     /gene="yehA"
                     /locus_tag="ACHK74_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFP0220264.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFDWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSSVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(4907..5245)
                     /gene="rcnB"
                     /locus_tag="ACHK74_001412"
     CDS             complement(4907..5245)
                     /gene="rcnB"
                     /locus_tag="ACHK74_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFP0220265.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5464..6288)
                     /gene="rcnA"
                     /locus_tag="ACHK74_001413"
     CDS             complement(5464..6288)
                     /gene="rcnA"
                     /locus_tag="ACHK74_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFP0220266.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPRHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDHEHHHHDHEDHHDHGHHHHHEHVEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLITVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            6409..6681
                     /gene="rcnR"
                     /locus_tag="ACHK74_001414"
     CDS             6409..6681
                     /gene="rcnR"
                     /locus_tag="ACHK74_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFP0220267.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6773..6869
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6904..7692
                     /gene="thiM"
                     /locus_tag="ACHK74_001415"
     CDS             6904..7692
                     /gene="thiM"
                     /locus_tag="ACHK74_001415"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFP0220268.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7689..8489
                     /gene="thiD"
                     /locus_tag="ACHK74_001416"
     CDS             7689..8489
                     /gene="thiD"
                     /locus_tag="ACHK74_001416"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFP0220269.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8554..9372
                     /gene="yegX"
                     /locus_tag="ACHK74_001417"
     CDS             8554..9372
                     /gene="yegX"
                     /locus_tag="ACHK74_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFP0220270.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            9424..10170
                     /gene="yegW"
                     /locus_tag="ACHK74_001418"
     CDS             9424..10170
                     /gene="yegW"
                     /locus_tag="ACHK74_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP0220271.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10144..11109)
                     /gene="yegV"
                     /locus_tag="ACHK74_001419"
     CDS             complement(10144..11109)
                     /gene="yegV"
                     /locus_tag="ACHK74_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFP0220272.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11106..12110)
                     /gene="yegU"
                     /locus_tag="ACHK74_001420"
     CDS             complement(11106..12110)
                     /gene="yegU"
                     /locus_tag="ACHK74_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFP0220273.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPIAELENNGVTNGAAMRVSPLGCLLPAHDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(12107..13384)
                     /gene="yegT"
                     /locus_tag="ACHK74_001421"
     CDS             complement(12107..13384)
                     /gene="yegT"
                     /locus_tag="ACHK74_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFP0220274.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            13641..14693
                     /gene="fbaB"
                     /locus_tag="ACHK74_001422"
     CDS             13641..14693
                     /gene="fbaB"
                     /locus_tag="ACHK74_001422"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFP0220275.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            15001..15855
                     /gene="gatY"
                     /locus_tag="ACHK74_001423"
     CDS             15001..15855
                     /gene="gatY"
                     /locus_tag="ACHK74_001423"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFP0220276.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKVGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            15884..17146
                     /gene="gatZ"
                     /locus_tag="ACHK74_001424"
     CDS             15884..17146
                     /gene="gatZ"
                     /locus_tag="ACHK74_001424"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFP0220277.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            17156..17608
                     /gene="gatA"
                     /locus_tag="ACHK74_001425"
     CDS             17156..17608
                     /gene="gatA"
                     /locus_tag="ACHK74_001425"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFP0220278.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            17639..17923
                     /gene="gatB"
                     /locus_tag="ACHK74_001426"
     CDS             17639..17923
                     /gene="gatB"
                     /locus_tag="ACHK74_001426"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFP0220279.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            17927..19282
                     /locus_tag="ACHK74_001427"
     CDS             17927..19282
                     /locus_tag="ACHK74_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFP0220280.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDTIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            19330..20370
                     /gene="gatD"
                     /locus_tag="ACHK74_001428"
     CDS             19330..20370
                     /gene="gatD"
                     /locus_tag="ACHK74_001428"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP0220281.1"
                     /translation="MKSVVNDTDGVVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVNRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            20476..21249
                     /locus_tag="ACHK74_001429"
     CDS             20476..21249
                     /locus_tag="ACHK74_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP0220282.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(21331..22230)
                     /gene="yegS"
                     /locus_tag="ACHK74_001430"
     CDS             complement(21331..22230)
                     /gene="yegS"
                     /locus_tag="ACHK74_001430"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFP0220283.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIILLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVATALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            22636..22953
                     /gene="yegR"
                     /locus_tag="ACHK74_001431"
     CDS             22636..22953
                     /gene="yegR"
                     /locus_tag="ACHK74_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFP0220284.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(23283..24644)
                     /gene="yegQ"
                     /locus_tag="ACHK74_001432"
     CDS             complement(23283..24644)
                     /gene="yegQ"
                     /locus_tag="ACHK74_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFP0220285.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(24747..25043)
                     /locus_tag="ACHK74_001433"
     CDS             complement(24747..25043)
                     /locus_tag="ACHK74_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0220286.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            complement(25045..25341)
                     /locus_tag="ACHK74_001434"
     CDS             complement(25045..25341)
                     /locus_tag="ACHK74_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFP0220287.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            complement(25550..25882)
                     /gene="yegP"
                     /locus_tag="ACHK74_001435"
     CDS             complement(25550..25882)
                     /gene="yegP"
                     /locus_tag="ACHK74_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFP0220288.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            complement(26073..26795)
                     /gene="baeR"
                     /locus_tag="ACHK74_001436"
     CDS             complement(26073..26795)
                     /gene="baeR"
                     /locus_tag="ACHK74_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFP0220289.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRVKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(26792..28195)
                     /gene="baeS"
                     /locus_tag="ACHK74_001437"
     CDS             complement(26792..28195)
                     /gene="baeS"
                     /locus_tag="ACHK74_001437"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFP0220290.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(28192..29607)
                     /gene="mdtD"
                     /locus_tag="ACHK74_001438"
     CDS             complement(28192..29607)
                     /gene="mdtD"
                     /locus_tag="ACHK74_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0220291.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNHRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(29608..32685)
                     /gene="mdtC"
                     /locus_tag="ACHK74_001439"
     CDS             complement(29608..32685)
                     /gene="mdtC"
                     /locus_tag="ACHK74_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFP0220292.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSIQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPCEQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANNLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(32686..35808)
                     /gene="mdtB"
                     /locus_tag="ACHK74_001440"
     CDS             complement(32686..35808)
                     /gene="mdtB"
                     /locus_tag="ACHK74_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFP0220293.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(35808..37055)
                     /gene="mdtA"
                     /locus_tag="ACHK74_001441"
     CDS             complement(35808..37055)
                     /gene="mdtA"
                     /locus_tag="ACHK74_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFP0220294.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSTTTSEEKATSREYAKKGARS"
     gene            37334..37390
                     /locus_tag="ACHK74_001442"
     CDS             37334..37390
                     /locus_tag="ACHK74_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP0220295.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            37662..37718
                     /locus_tag="ACHK74_001443"
     CDS             37662..37718
                     /locus_tag="ACHK74_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP0220296.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            37991..38050
                     /gene="ibsA"
                     /locus_tag="ACHK74_001444"
     CDS             37991..38050
                     /gene="ibsA"
                     /locus_tag="ACHK74_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFP0220297.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            38271..38930
                     /gene="yegL"
                     /locus_tag="ACHK74_001445"
     CDS             38271..38930
                     /gene="yegL"
                     /locus_tag="ACHK74_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP0220298.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIA
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            38927..39688
                     /gene="pphC"
                     /locus_tag="ACHK74_001446"
     CDS             38927..39688
                     /gene="pphC"
                     /locus_tag="ACHK74_001446"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFP0220299.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWAE"
     gene            complement(39753..40214)
                     /gene="yegJ"
                     /locus_tag="ACHK74_001447"
     CDS             complement(39753..40214)
                     /gene="yegJ"
                     /locus_tag="ACHK74_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HFP0220300.1"
                     /translation="MKKILLMLSLLFFTTAGFSEVSDTLVTGGYDKQAMSDAIKHARK
                     ETDKFIEVMNKKDADTFAVKAPITDHGRTEHFWLTDVTYSNGMFIGVISNDPGIVTNV
                     EYGQEWKIKKEDISDWMYTRGDKIYGGYTIDPLLVTYPKEEADELRAKLVR"
     gene            40412..42358
                     /gene="yegI"
                     /locus_tag="ACHK74_001448"
     CDS             40412..42358
                     /gene="yegI"
                     /locus_tag="ACHK74_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFP0220301.1"
                     /translation="MKTNIKVFTSTGEMTTLGLELGKGGEGAVYDINEFVDSVAKIYH
                     TPPPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMI
                     YSPAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLI
                     DSDSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTKNHDNFGLALLIFHVLFG
                     GRHPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMF
                     QQAFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCTLDNQGV
                     IYFIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRRE
                     YIILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEIQQRREAFN
                     RAKMDYDHLVSQSQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQ
                     KQKFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVID
                     WKASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPART
                     MPLMEPLRQAAEKLAQAQADLSRC"
     gene            complement(42370..43722)
                     /gene="yegD"
                     /locus_tag="ACHK74_001449"
     CDS             complement(42370..43722)
                     /gene="yegD"
                     /locus_tag="ACHK74_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0220302.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            43856..44713
                     /gene="alkA"
                     /locus_tag="ACHK74_001450"
     CDS             43856..44713
                     /gene="alkA"
                     /locus_tag="ACHK74_001450"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFP0220303.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            complement(44752..48069)
                     /gene="dgcE"
                     /locus_tag="ACHK74_001451"
     CDS             complement(44752..48069)
                     /gene="dgcE"
                     /locus_tag="ACHK74_001451"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP0220304.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRMLLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMMAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQKFRRSFS
                     DPALSHALDRRVFHEFFQLAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            48387..49028
                     /gene="udk"
                     /locus_tag="ACHK74_001452"
     CDS             48387..49028
                     /gene="udk"
                     /locus_tag="ACHK74_001452"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFP0220305.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            49120..49701
                     /gene="dcd"
                     /locus_tag="ACHK74_001453"
     CDS             49120..49701
                     /gene="dcd"
                     /locus_tag="ACHK74_001453"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFP0220306.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            49723..51576
                     /gene="asmA"
                     /locus_tag="ACHK74_001454"
     CDS             49723..51576
                     /gene="asmA"
                     /locus_tag="ACHK74_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFP0220307.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVKMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(51850..53433)
                     /gene="yegH"
                     /locus_tag="ACHK74_001455"
     CDS             complement(51850..53433)
                     /gene="yegH"
                     /locus_tag="ACHK74_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP0220308.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEVEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
BASE COUNT        13363 a        13311 c        13925 g        12965 t
ORIGIN      
        1 ttgctgccgc tgtcttttct tcttttttta tgagcgctgg agtatttgct gcagacgttg
       61 ataccggaac attaactatt aaggggaata ttgcagaatc tccgtgtaaa ttcgaagcgg
      121 gtggtgattc agtaagtatt aatatgccga ctgtaccaac cactgtcttt gaaggtaaag
      181 ctaaatattc tacctatgat gatgcagtcg gtgtaaccag cagaatgtta aaaattagct
      241 gcccgaaaga agttgctggt gtaaaactct cgttgattac caacgataaa ataaccggta
      301 acgataaggc gatagccagt agcaacgata ccgtgggtta ctatctctat ttaggtgata
      361 acagcgatgt cctggatgtt tctgcacctt ttaacattga gagttataaa acagcggaag
      421 gtcaatatgc tattccgttt aaagcaaaat acctgaaact gacagataac tcagtgcaat
      481 caggtgatgt gttatcttct ctggttatgc gtgtggcgca ggattaataa ataaaattgt
      541 aagtttctcc aggtaataaa catggccgct atcccatggc ggccttttaa tttaagaggc
      601 attaaaatga aaggattatt atctttactc attttttcta tggtccttcc tgcacatgcc
      661 ggaattgtta tctacgggac gcgcattatt tacccggcag aaaataaaga agttatggtg
      721 cagttgatga accagggaaa ccgttcttcg ctgctgcagg cgtggattga tgatggcgat
      781 acgtcgttac caccagaaaa aattcaggtt cctttcatgt taacgccacc agtggcaaaa
      841 ataggggcaa attccgggca gcaagtaaaa atcaaaatta tgccgaataa actgcccact
      901 aataaagaaa gcatttttta tctgaatgtt ctggatattc caccaaatag tccagagcaa
      961 gaaggcaaaa atgcactgaa gtttgcgatg caaaacagaa ttaagttgtt ttaccggcca
     1021 gcgggtattg ctccggtaaa taaagcgacc tttaaaaagt tactggtaaa tcgcagtggc
     1081 aatggattgg tgataaaaaa tgactcagct aattgggtga cgatttcgga tgtcaaagct
     1141 aataatgtta aagtcaatta tgaaactatt atgattgccc ccttagaaag tcagagtgtt
     1201 aatgtcaaaa gtaataatgc aaataactgg tatctgacca ttatcgatga ccacggcaac
     1261 tatattagtg acaaaattta attgcaggag ctgcctatgt tgagaatgac cccacttgca
     1321 tcagcaatcg tagcgttatt gctcggcatt gaagcttatg cagctgaaga agcctttgat
     1381 acccatttta tgataggtgg aatgaaagac cagcaggttg caaatattcg tcttgatgat
     1441 aatcaaccct taccggggca gtatgacatc gatatttatg tcaataagca atggcgcggg
     1501 aaatatgaga ttattgttaa agacaacccg caagaaacat gtttatcaat agaagttatc
     1561 aagcggttag gcattaatag cgataacttc gccagcggta agcaatgttt aacatttgag
     1621 caacttgttc agggtgggag ctatacctgg gatatcgggg tttttcgtct cgatttcagt
     1681 gtcccgcagg cctgggtgga agaactggaa agtggctatg ttccaccgga aaactgggag
     1741 cggggtatta atgcgtttta tacctcttat tatgtgagtc agtattacag tgactataaa
     1801 gcgtcgggta atagtaagag tacatatgta cgttttaaca gcgggttaaa tttactgggg
     1861 tggcaactgc attctgatgc cagtttcagt aaaacaaata acaatccagg ggtgtggaaa
     1921 agcaataccc tgtatctgga acgtggattt gcccaacttc tcggcacgct tcgcgtgggt
     1981 gatatgtaca catcaagcga tatttttgat tctgttcgct tcagcggtgt gcggttgttt
     2041 cgtgatatgc agatgttgcc taactcgaaa caaaatttta cgccacgggt gcaggggatt
     2101 gctcagagta atgcgctggt aactattgaa cagaacggtt ttgtggttta tcagaaagag
     2161 gttcctcctg gcccgttcgc gattgcagat ttgcagttgg ccggtggtgg agcagatctt
     2221 gatgtcagcg tgaaagaggc ggacggctcg gtaaccacct atctggtgcc ttatgcagca
     2281 gtgccaaata tgctgcaacc cggcgtatcg aaatatgatt ttgcggcggg tcgtagccat
     2341 attgaagggg cgagcaaaca aagtgatttt gtccaggcgg gttatcagta tggttttaat
     2401 aatttattga cgctgtatgg tggctcgatg gtcgcgaata attattacgc gtttactttg
     2461 gggactggct ggaatacacg cattggtgcc atttccgtcg atgccacgaa gtcgcatagt
     2521 aaacaagaca acggcgatgt gtttgacggg caaagttatc aaattgccta caacaaattt
     2581 gtgagccaaa cgtcgacgcg ttttggtctg gcggcctggc gttattcatc gcgtgattac
     2641 cggacattta atgatcacgt ttgggcaaac aataaagata attatcgccg tgatgaaaac
     2701 gatgtctatg acattgccga ttattaccag aacgattttg gccgtaaaaa tagcttctct
     2761 gccaatatga gccagtcatt gccagaaggc tgggggtctg tgtccttaag tacgttatgg
     2821 cgagattact gggggcgtag cggcagcagt aaggattatc agttgagtta ttccaataac
     2881 tggcggcgga taagctatat cctcgcggca agccaggcgt atgacgagaa tcatcatgaa
     2941 gagaaacgtt ttaatatttt tatatcgatt ccctttgatt ggggtgatga cgttacgacg
     3001 cctcgtcggc aaatatatat gtctaactca acgacgtttg atgatcaggg gtttgcctca
     3061 aataatacgg gattatcagg aaccgttgga agccgggatc agtttaacta tggggtcaac
     3121 ctgagttatc agaatcaggg aaatgaaacg acagctgggg cgaatttgac ctggaacgcg
     3181 ccggttgcga cagtgaatgg cagttatagt cagtcgagta cttatcgaca ggctggagcc
     3241 agtgtttcag ggggcattgt cgcctggtcg gggggcgtta atctggcgaa ccgtctttca
     3301 gaaacgtttg ctgtgatgaa tgcgccagga attaaagatg cttatgtcaa tgggcaaaaa
     3361 tatcgcacaa caaaccgtaa tggagtggtg gtatacgacg gaatgacacc ttatcgggaa
     3421 aatcacctga tgctggatgt gtcgcaaagc gatagcgaag cagaattacg tggcaaccgg
     3481 aaaattgccg ccccttatcg cggcgcggtt gtactggtta attttgatac cgatcagcgc
     3541 aagccctggt ttataaaagc gttaagagcg gatgggcaac cattaatgtt tggttatgaa
     3601 gtcaatgata tccatggtca taatattggc gttgtcggtc agggaagcca gttatttatt
     3661 cgcaccaatg aagtaccgcc atcggttaat gtagcaattg ataagcaaca aggactttca
     3721 tgcacaatca ccttcggtaa agagattgat gaaagtagaa actatatttg ccagtaatag
     3781 tcaggtggtt atgaatattc gtataatgtt atttatagta atgatgatgg ttatgcctgt
     3841 gagctatgcg gcatgttata gtgagttatc tgttcagcac aatttggttg ttcaggggga
     3901 ttttgcactt actcaaacac aaatggcgac atatgagcat aattttaatg attcgtcatg
     3961 cgtgagtaca aatactatca cccctatgag cccgtcggat attattgttg gactttataa
     4021 cgataccatt aaattaaatt tacattttga ttggaccaat aaaaacaaca tcacgttatc
     4081 aaataatcag accagtttca ccagtggtta ttcggtaacg gtgacacctg cggccagtaa
     4141 tgcaaaagtg aatgtttctg cggggggcgg cggttcagtg atgattaatg gagttgcgac
     4201 attatccagt gcttcatcat cgacacgcgg gagtgccgca gtacaatttc tactgtgttt
     4261 attaggtggc aagtcatggg atgcatgtgt aaatagctac agaaatgcat tggcacaaaa
     4321 tgcaggtgtc tattccttta atctgacatt gtcatacaac ccgataacca caacctgcaa
     4381 accggacgat ttattaatta ctttagacag tattcccgtt tcacaattac cagccacagg
     4441 taacaaagca acaataaata gtaaacaagg ggatattatt ctgcgttgta aaaatttatt
     4501 aggtcaacaa aatcaaacat cacggaaaat gcaggtgtat ttatcaagtt ctgacttgtt
     4561 aaccaacagc aacacaatac tgaaaggtgc ggaagataat ggcgtaggat ttattcttga
     4621 aagtaatggt tcgtcagtca cacttttaaa tatcactaac agcagtaaag gatacacaaa
     4681 tttaaaagaa attgcggcaa agtcaaaact tacagataca acggtttcaa ttccgataac
     4741 tgccagttac tacgtctacg atacaaacaa agttaaatct ggcgcactgg aggcaaccgc
     4801 attaatcaac gtgaaatacg actaataaca agcaagacga gcaagtggct aataataaaa
     4861 aataacaagg tgtctgcatt tccccctcat gatgaggggg ctttttttag cgatgataaa
     4921 aaatctcacc gtcgtaggct ttaatgattt taccgtcggt gtcgctgatc aacacgtacg
     4981 cgccacccat ataggtccag tgcgtcccgg catcaggcgc gggcaggtta cgtaactgcc
     5041 actgcttaat gttgtactca tcggtgcggt acatctctgg gacggtatcg cctggtttga
     5101 aatgcttaaa gtcagcaatg aaactgctta actcatactt agaaattccc gaggtattgg
     5161 tcgaacccgc ggtggctggt gcggcccagg cggcactggt aaccagcaaa agcgcaccca
     5221 gcaacatctt atttttaatg gtcataaact ctccactttg gggatctgta atatcagtcg
     5281 catatctttc ctgccataca atataactga caagcgatat tttgttagtg atcccatgat
     5341 ttcgtaaaaa gtgtaaatga aaggatgccc taaaagcatc cttagcgagc agagagtcag
     5401 cggaaatagc tgatttaaga atcatcctgg gctgaagata acatgcaagc ctaaaggatt
     5461 gagttatcgc attatgccca tgaagccatg tacgcccata tacacaccga ctaagccaat
     5521 caacagactg gaaaaatagg gggcgcgttt agcgagagtg ttaaatccgc tccagcgttt
     5581 tgcgacctgc tgaacgctga ttgctgcgcc aacgcctacg gtgataagcg ttaacgccag
     5641 gccaatgctg aaactgacga ccagtgttgc gcccagtgtc agggctttca actgaatgca
     5701 tatcaacagc acggtaattg ctgccgggca ggggataagg ccaccggtta agccaaataa
     5761 caaaatttgc cagttggtga cctctctacc atcaaagcgt cgtttaatgt cattggcatg
     5821 ggctcgtgca tgggcatcct gatactcgac atgttcgtga tggtgatgat gtccatggtc
     5881 gtggtgatct tcgtgatcgt ggtgatgatg ttcatgatca tactcgtgca tattctccag
     5941 ccagttgcgt tcgccgcgcc aggtacgcca gaacatccag aacgcggtgc caataatgat
     6001 cactgcggaa atcagctgga gccacggttc tgctgattga gcagtaaagc gcttgctgat
     6061 caccatcccg ccaaaggcaa ttaaccagac cactgcggta tgcgaaatag ttgctgccag
     6121 tccgagcatc accgcttgtt taatggtgcc tttgatggcg atgataaacg ccgccatcat
     6181 cgtttttgag tgccttggtt ccaggccatg aagcgcacca agtaagatgg cgctggggat
     6241 gaagaaccag gcgtttcctt gctgaagaag agttgtaaat tcggtcatga taataattct
     6301 tagtattaat tcggcaatct gattctactc ccccccagta cctgatacta ccccccagta
     6361 gattaatagt gctatgattt ttcatattct tgttaacaag gtgttgccat gtctcataca
     6421 atccgtgata aacagaaact gaaagcgcgt gccagtaaga ttcagggcca ggtcgtggcg
     6481 ctcaagaaaa tgctcgacga gccgcacgaa tgcgctgcag ttttacaaca gattgctgct
     6541 atccgtggcg cggtaaacgg tctgatgcgg gaagtgatta aaggtcatct gacggaacac
     6601 atcgttcacc agggggatga gctaaaacgt gaagaagatc tggatgtcgt tctgaaggtg
     6661 ctggattcat atatcaaata atttattaac gcgattgtaa aactgccgtt tttcctcgtt
     6721 tacaacgcgt gcgctggaca ttaccatcct cctctgcgat ttatcatcgc aaccaaacga
     6781 ctcggggtgc ccttctgcgt gaaggctgag aaatacccgt atcacctgat ctggataatg
     6841 ccagcgtagg gaagtcacgg accaccaggt cattgcttct tcacgttatg gcaggagcaa
     6901 actatgcaag tcgacctgct gagttcagcg caatctgcgc acgcgttaca cctttttcac
     6961 caacattccc ctcttgtgca ctgcatgacc aacgatgtgg tgcaaacctt tactgccaat
     7021 accttgctgg cgctcggtgc atcgccagcg atggttatcg aaaccgaaga ggccagtcag
     7081 tttgcggcta tcgccagtgc cttgttgatt aacgttggca cactgacgca gccacgcgct
     7141 caggcgatgc gtgctgccgt tgagcaagca aaaagctctc aaacaccctg gacgcttgat
     7201 ccagtagcgg ttggtgcgct cgattatcgc cgccattttt gtcatgaact tttatctttt
     7261 aaaccggcag cgatacgtgg taatgcttcg gaaatcatgg cattagctgg cattgctaat
     7321 ggtggacggg gagtggatac cactgacgcc gcagctaacg cgatacccgc tgcacaaaca
     7381 ctggcacggg aaactggcgc aatcgtcgtg gtcactggcg aggtggatta tgttaccgat
     7441 ggacatcgta tcattggtat tcacggtggc gatccgttaa tgaccaaagt ggtaggaact
     7501 ggctgtgcat tatcggcggt tgtcgctgcc tgttgtgcct taccaggcga tatgctggaa
     7561 aatgtcgcat ctgcctgtca ctggatgaaa caagccggag agcgcgcagt cgccagaagc
     7621 gaggggccag gcagttttgt tccacatttc cttgatgcgc tctggcaatt gacgcaggag
     7681 gtgcaggcat gaaacgaatt aacgctctga cgattgccgg tactgatccc agtggtggtg
     7741 cggggattca ggccgatctt aaaaccttct cggcacttgg cgcttatggt tgctcagtta
     7801 ttactgcact ggtggcgcaa aatacccgtg gcgtacagtc ggtgtatcgc attgagcctg
     7861 attttgtcgc cgcccagctc gattcggtgt tcagcgatgt gcgaatcgac accactaaaa
     7921 tcggtatgtt ggcggaaacc gatattgttg aagcggtggc agaacggttg caacgttatc
     7981 agatccaaaa cgtggtactc gacaccgtta tgctggcaaa aagcggcgac ccgctgcttt
     8041 caccttcggc ggttgctacg ctgcgcagtc gattattgcc acaggtttca ttaataacgc
     8101 caaacttgcc cgaagctgcc gccttgctcg acgcgccaca cgcgcgcacc gaacaggaaa
     8161 tgctggaaca agggcgatcg ctgttggcga tgggctgtgg cgcagtgcta atgaaaggtg
     8221 gtcatctgga tgatgagcaa agcccggact ggctgtttac ccgcgagggt gaacaacggt
     8281 ttaccgcacc gcgcattatg accaaaaaca cccacggcac tggttgtacg ctctctgcgg
     8341 cgttggctgc actacgcccg cgccatacaa actgggctga caccgtacag gaggcaaaaa
     8401 gctggctttc atcggcgtta gcccaggccg acacgctgga agttggtcac ggtattggtc
     8461 cggttcacca cttccacgcc tggtggtgag caccgtacta tagctgcttt ggttattgtc
     8521 agcctttggc actgacattc agggatgaaa ccaatgcagc taagaattac cagtcgaaaa
     8581 aaactcacct ctctattatg cgctcttggg ctaatttcca tcgttgcaat ttatccgcgt
     8641 cagacggtga attttttcta ttcgacagca gttcagatta cagactacat ccatttctac
     8701 ggttatcgcc cggttaaatc tttcgctatt cgcattcctg ccagttacac cattcacgga
     8761 atagatgttt ctcgctggca ggagcggatc gactggcagc gggtggcaaa aatgcgcgac
     8821 aacggtatcc gcttacagtt tgcttttatt aaggcgacgg aaggcgaaaa gctggtggac
     8881 ccgtattttt cgcgtaactg gcaactaagc cgcgaaaatg gcctgctgcg cggggcgtat
     8941 cattatttct ccccgtcggt agcagcgccg gttcaggcac gattatttct gcaaacggtg
     9001 gatttctcac aaggcgattt ccctgcggta ctggacgtag aagagcgggg aaaattatcg
     9061 gcaaaagaat tacgcaagcg ggtaagtcag tggctaaaaa tggtcgaaaa acgcacggga
     9121 aaaaagccga ttatttactc aggagccgtt ttttatcaca cgaatctggc gggctatttt
     9181 aatgaatatc cgtggtgggt ggctcactac tatcaacgtc gtccggacaa tgacggcatg
     9241 gcctggcgtt tctggcagca ttccgaccgt ggacaggtag atggcatcaa tggtccggtg
     9301 gattttaatg tgtttaatgg cacggtggag gagttgcagg gattcgttga tgggattaaa
     9361 gaaacgcctt aaataagaag cagtgatata ctcccaccag actccctggc aggaaaaaga
     9421 caaatggaac aagcgcatac ccagctgatt gcccaactga acgagcgtat tttagctgcg
     9481 gataacacgc cgctttatat taagtttgcc gaaacggtaa aaaatgccgt gcgcagcggg
     9541 gtgctggagc atggcaatat tttgcccggt gagcgtgatt taagtcagtt aaccggcgtt
     9601 tcgcgcatta cggtgcgcaa ggcgatgcag gcgctggaag aagagggtgt ggtgacgcgt
     9661 tcgcgcggtt acggcacgca gatcaacaac atcttcgaat actcgttgaa agaagcgcga
     9721 ggtttttctc agcaggtggt attgcgcggg aaaaagcccg atacgctatg ggttaacaag
     9781 cgtgtcgtga aatgtcccga agaagtcgcg cagcagctgg cggtcgaagc aggaagtgat
     9841 gtctttttgc ttaagcgtat tcgctatgtc gatgaagaag cggtatcgat tgaggaatcg
     9901 tgggttccgg cgcatttaat tcatgatgtt gatgccatcg ggatttcact ttatgactat
     9961 ttccgcagcc agcacattta cccacaacgt acacgttccc gcgttagcgc ccggatgccg
    10021 gatgccgagt ttcagtcaca tattcagtta gatagcaaaa taccggtgct ggtgatcaag
    10081 caagtggcgc ttgaccaaca gcaacggcct attgagtaca gcattagcca ctgtcgcagc
    10141 gatctatacg tttttgtgtg cgaggagtag ttcctcgcgc gttggcgcac aatcaccgcc
    10201 ccgatgcccg acaacccacg acgccactgc attgcccagt aatacggcat ccgccagtgg
    10261 cagaccagag gccagcccgg caagtacgcc acctgcatga ctgtcgcccg ccccaatggt
    10321 gtctacaact tgcgttggaa atgccggaat gcagccagaa gcgtcgttgc tgaaatacca
    10381 tgcaccctct ttatcgagac gaacgatcaa cggcgcggca aatttctcct gccattgctt
    10441 gccaagtgtt gttatctctg cggataaagc aaaacgttcg gcggcaatct cagcctcttg
    10501 acgattgagt gatactaaag gtcgacaggc catgatccgc gccagtaatg cgtccgggat
    10561 atcgccgata cgtgggccaa aatcgataaa cggcgtcacg tcttgcagct tttccagcca
    10621 ttccactaac aattcgccgc agggcgaggc cagttgataa ccggaaaaat agagcaggct
    10681 gccaggcgca acggttaatc gcgccagcca ctggcgattc cactgatttt ccacaccgct
    10741 gaatgacata aaagtgcgtt cgccatccgg ctcaaccagc gccagacacc agccgttatc
    10801 gccttcggcg ttatcgatca ggctgattaa cccctctttc gccatccggt tgcgaataat
    10861 ctccgcccac acgccctgac cgagcggcaa ggcattaccc gcttcgatgc cgaggcgctt
    10921 taacgccacg gcaatattca gtgcgcagcc gccaacgtta acgctctgct gcttcagttc
    10981 gatatcgcac ccacgccagg ggagggcata agcgtcggcg atcacatcaa tgaccgccgc
    11041 gccgacgacc atcaccggct gacgcgaggt taattcaggc agcagcgtgt gtaatcgagc
    11101 gccgctcata ccgcctcccg ttgttgacga tatttcgcca gcgctgtggc atagcggttg
    11161 aaatcaagct gatttaccgc atccagttcc gcctttaatg caggatcgat agcgttaacg
    11221 ccatgcaacg cgccgcaaat cgccgtcgcc atagcgccga tggtgtctgt gtcgccgcca
    11281 aggttagcgc acaggacggc gcagcgattc gggtcggttt gtgccagttc aaccagcgca
    11341 atggcgcacg gaacggactc aatagtgctg gtacctgcgc caacgacctg gtaaagctgt
    11401 tcgctggcgg attcggtgcc gtcggcattg cgcacaattt tcagcgcaat ctccagacgt
    11461 gctgccagtg aggcgctgaa cgtggtgatg cgtttttgct gtgcatgtcg cgcaattgaa
    11521 ggcagtgaat ctacaatcgc tgaccagctt tctccgtcaa tggcacgaga aatcgcccat
    11581 gcgatgacta ccgcgcctgc aaccgccaga tcggatttat gggtcgggct ggacgccagc
    11641 gccacatcat caataaagga atcaacatcg tgcgccggaa gcaaacaacc taatggcgag
    11701 acgcgcatcg ctgcgccgtt ggtcacgccg ttgttttcca gttcagcaat gggtttaccg
    11761 tcgcgaatgg cgttaagcgc aatcttcgag gtcggaccaa gtacgttttt gttaaaggcg
    11821 tcgaaacgca gcgcccagtc gagaatatta cgcccaatca gattcggatc gatcttgcct
    11881 tcacgttcca gtaacgcatc cgccagacac agcgccatcg aggtatcgtc ggtgaattcg
    11941 gcgcggttaa aataacaggc cgcgttattc tcctttggtc caggaagaaa acggtcaatc
    12001 cagccaaagt gtgctttaac gcggctgcgt ggccaaagct cggagggcat ccccatcgca
    12061 tcccctaacg cctgcccata aagagcaccg agaatacgtt ctgttttcat ttaacttccc
    12121 cttgtgtcaa cgcaatatcg cgatcatcga ccttgatagc cgtaatttcg ttgtcggatt
    12181 cgcggaaaaa aatcatgaac agcacggcga taatggcaat catcaccgcg ccaaaagtcc
    12241 acatcccgga ccagttgaaa gtcagtccgt ttaccggttc ctgataagcg aacatctttt
    12301 ccatcatcac accgccaaga cgatagccga gcaaactgcc gaagccctgg cagcagagcg
    12361 tgatcagccc ctgcgcggcg gtacgcatat gcacgggggc ttttttatcg acatagatgt
    12421 aagcggtaac gtagtaaaaa tcgtaactta cgccgtgaag caaaataccg aggaacagta
    12481 acgcgtaggt gaaatattca tccgcactac cgtaaataaa gaagccatag cggatcgcag
    12541 cggtgaccag accaagcaat aatacctttt tgataccaaa gcgtttagtg aaaaacggta
    12601 atgccagcat aaagaatatt tcagagaact ggccgagcgt catccagccg gtggcgtttt
    12661 tcatgccaac ttcggtcaga taaccgttgg caaagatgta atagaacgcc agtggcatcg
    12721 caaacaggaa tgaacagaag aaaaagacga ggaagttttt atcgcgcagc aggatcagcg
    12781 catccaggcc gagcatgact ttaatgtcca ttttgccggt gctttttggc ggcgtgtcgg
    12841 gcaggaaaaa cgcaaacaca ccgagcagag cagaacttcc ggcggtaatc agcagcggga
    12901 tgttagtcgg tgagatatcg gcatacccca gtatttgcgg caagaaacca catgccagac
    12961 cggaggcaat ccagccgata gtgcccatca cacgaatgcg cgggaaatca cgctcaacat
    13021 ccggcacgtt ggcaaaagcg atgctgttag tcagcgcaat ggtcggcata taggttagcg
    13081 agtaggccag cagtaacggg aagaacccgg caaaagtggt ctgttgcgca gcgaaataca
    13141 tcagcacagc accagcgaac atcaataccg ccagcacttt ttgcgccgag aaaaagcggt
    13201 cagtgatgga gccaaccaga attggcgaca ggatcgccgc aatggcggta caggcatacg
    13261 accagccaat ttctccggca ctaaaaccgc ttttacttaa ccacaaccac aatggcacaa
    13321 accacgcgcc ccagataaac cattcaacaa acatcatgaa cgacagcttt gctgttgttt
    13381 tcatctttta attccttcat gacaggtaat gtaaggtacg cgatgacaat accacttaat
    13441 aatacctttt aaataccttt acggtgaaaa cttgaccgcg ctaacatttt ttctgatgaa
    13501 tcgagccaac agaaaacgct gaaaaaacat ccaaaagatg gaaaaactcg cccgtaacga
    13561 ccaggattat gattgcccgc aaaaggcggg ccaggacaat ccatagccga tatccaatcg
    13621 gaatttacgg gagcatagta atgacagata ttgcgcagtt gcttggcaaa gacgccgaca
    13681 accttttaca gcaccgttgt atgacaattc cttctgacca gctttatctc cccggacatg
    13741 actacgtaga ccgcgtaatg attgacaata atcgcccgcc agcggtgtta cgtaatatgc
    13801 agacgttgta caacaccggg cgtctggctg gcacaggata tctttctatt ctgccggttg
    13861 accagggcgt tgagcactct gccggagctt catttgctgc taacccgctc tactttgacc
    13921 cgaaaaacat tgttgaactg gcgatcgaag cgggctgtaa ctgtgtggca tcaacttacg
    13981 gcgtgttggc gtcggtatcg cggcgctatg cgcatcgcat tccatttctc gtcaaactta
    14041 atcacaacga gacgctaagt tacccgaata cctacgatca aacgctgtat gccagcgtgg
    14101 agcaggcttt caacatgggc gcggtggcgg ttggtgcgac tatctatttt ggctcggaag
    14161 agtcacgtcg ccagattgaa gaaatttctg cggcttttga acgtgcgcat gagctgggca
    14221 tggtgaccgt gctgtgggcc tatttgcgta actccgcctt taagaaagat ggcgttgatt
    14281 accatgtttc cgccgacctg accggtcagg caaaccatct ggcggcaacc atcggtgcag
    14341 atatcgtcaa acaaaaaatg gcggaaaata acggcggcta taaagcaatt aattacggtt
    14401 acaccgacga tcgtgtttac agcaaattga ccagcgaaaa cccgattgat ctggtgcgtt
    14461 atcagttagc taactgctat atgggtcggg ctgggttgat aaactccggc ggtgctgcgg
    14521 gcggtgaaac tgacctcagc gatgcagtgc gtactgcggt tatcaacaaa cgcgctggcg
    14581 gaatggggct gattcttgga cgtaaggcgt tcaagaaatc gatggctgac ggcgtgaaac
    14641 tgattaatgc cgtgcaggac gtttatctcg atagcaaaat tactattgcc tgatacgcct
    14701 catacgaaca gcaccacccc gtaggacgga taaggcgttt acactgcatc cggcaaccta
    14761 tgcctgatgc gacgcggaag cgtcttatca tgcctacata gcactgccac gtatgtttac
    14821 accgcatccg gcataaaaac acgcgcactt tgctacggct tccctatcgg gaggccgttt
    14881 ttttgccttt cactcctcga acaattttca cattgtcgtt tttgtgatcg ttatctcgat
    14941 atttaaaaac aaataatttc attatatttt gaaatcgaaa acaaacgaca ggatatgaaa
    15001 atgtacgtgg tatcgacaaa gcagatgctg aacaacgcac agcgcggcgg ttatgcggtt
    15061 ccggcattca atattcacaa tctcgaaacg atgcaagtgg tggtggaaac cgctgccaac
    15121 ctgcatgcgc cggtcatcat cgccggaacg cctggcacat ttactcatgc tggtacagaa
    15181 aatctgttgg cgctggtcag cgcgatggcg aagcaatatc accatccact ggcaattcat
    15241 ctcgaccatc acacgaaatt tgacgatatc gctcagaagg ttcgttctgg cgtgcgctca
    15301 gtcatgattg acgcctcgca tttgcctttt gcgcaaaata tttcacgggt caaagaggtg
    15361 gtggattttt gccatcgctt tgatgtcagc gtcgaggcgg agctggggca acttggcggc
    15421 caggaagatg atgtgcaagt caatgaagcc gatgcgttgt acaccaaccc cgcacaggcg
    15481 cgtgaatttg ccgaggcaac cgggattgat tccctggcgg tcgccatcgg tacggctcac
    15541 ggtatgtatg ccagcgcacc ggtgcttgat ttttctcgac tggagaacat tcgccagtgg
    15601 gtgaacttac cgctggtgct gcatggcgcg tcaggattat cgactaagga tattcagcaa
    15661 accatcaaag tggggatatg caaaatcaac gttgcaacgg agctgaaaaa tgccttctcg
    15721 caggcgttaa aaaattacct gaccgagcac cccgaagcga ccgatccccg ggattatttg
    15781 cagtcggcta aatccgcaat gcgcgatgtg gtgagcaaag tgattgccga ttgtggctgc
    15841 gagggcaggg cataacgcac ctgccattta acaaggaaaa aacatgaaaa cgttaattgc
    15901 ccggcataaa gctggtgaac atatcggcat atgttcagtc tgttctgccc atccgttggt
    15961 tatcgaagcg gcgctggcat ttgatcgcaa cagcacgcgc aaagtgctga ttgaagcaac
    16021 gtcaaaccag gtcaatcaat ttggcggtta taccggaatg acaccggcag actttcgcga
    16081 atttgttttt acgattgccg ataaagttgg gtttgcacgc gaacgcatta ttctcggcgg
    16141 cgatcatctg gggccaaact gctggcagca agaaaatgcg gatgcggcga tggaaaaatc
    16201 cgtcgagctg gtaaaggaat atgttcgtgc cggcttcagt aaaattcatc ttgatgcgtc
    16261 aatgtcctgc gcgggggatc ccataccgtt agcaccagaa acggttgcgg aacgagctgc
    16321 tgtgctttgc tttgctgcgg aaagtgtggc gacagattgc cagcgtgagc aactgagcta
    16381 tgtcattggc accgaagttc cggttccggg cggtgaggcc agcgccattc agtcagtaca
    16441 catcacccat gttgaagatg ccgccaatac tttacgtacg catcaaaagg cctttattgc
    16501 ccgtgggctg acagaggcgt taacacgcgt gattgccatc gtggtgcagc cgggcgtgga
    16561 atttgatcac agcaatatta tccattatca gccgcaggaa gcgcagccgc tggcgcaatg
    16621 gatagaaaac acccgaatgg tttatgaagc acattctacc gattaccaga cccggacggc
    16681 ttattgggaa ttagtccgcg atcactttgc aatattgaaa gtcggtcccg cattaacctt
    16741 tgctttacgc gaggcgatat ttgcactggc acaaattgag caggaactta tcgcccctga
    16801 aaatcgcagc ggttgcctgg cggtaattga agaagtgatg ctcgacgaac cgcaatactg
    16861 gaaaaaatat tatcgtacgg gttttaacga ttcattactg gatattcgtt acagcctgtc
    16921 ggatcgtatt cgttattact ggccgcatag tcggattaaa aatagcgtcg aaacgatgat
    16981 ggtgaatctt gaaggcgtgg acatcccact gggcatgatt agtcagtatc ttcccaaaca
    17041 atttgaacgc attcagtctg gggaattatc agcaataccg catcagctga ttatggataa
    17101 aatttatgat gttttgcgcg cctatcgcta cggctgtgcg gaataaggac ggtatatgac
    17161 taacctgttt gttcgtagcg gaatttcttt tgtcgatcgt agcgaagttt taacccatat
    17221 cggtaatgag atgctcgcca aaggtgtggt tcatgatacc tggccacagg cattaattgc
    17281 cagagaagca gaattcccta ccgggataat gcttgagcag cacgccattg caataccgca
    17341 ttgtgaggcg attcatgcta agtcgtcagc catttatctg ttaaggccaa caaataaagt
    17401 tcattttcag caagcggatg atgataacga cgtggcggta tcgttggtta ttgcgttgat
    17461 tgtggaaaat ccgcagcagc aattgaaact tttacgctgt ttatttggca agttacaaca
    17521 gcccgatatc gtcgagacac taatcactct tcctgaaacc cagttaaagg aatacttcac
    17581 aaagtatgtt ttagattcag acgaataaat ccctctgtaa caataaaaag gactatttat
    17641 gaaacgcaag attattgtcg cttgcggagg cgcggttgcg acctctacga tggcggcgga
    17701 agaaattaaa gagttgtgtc agagtcataa tattcctgtt gaattaatcc agtgtcgggt
    17761 taatgaaata gaaacctata tggatggtgt gcatttgata tgtaccactg ccagggtgga
    17821 tcgcagtttt ggcgatattc cgttagttca cggcatgcct tttatttctg gtgtcggtat
    17881 cgaagcatta caaaataaaa ttctgactat cttacagggg tgacctatgt tttcagaagt
    17941 catgcgttat attctcgacc tcggccctac ggtgatgctt ccgattgtca tcattatttt
    18001 ttctaaaata ttaggcatga aggcaggcga ttgctttaaa gcgggtctgc atatcgggat
    18061 tggctttgtt ggcattggcc ttgtgattgg cttaatgctg gattccattg gtccggcggc
    18121 gaaagcgatg gcggaaaatt tcgacctgaa tctgcatgtg gtcgatgtcg gctggccggg
    18181 ctcttcacca atgacctggg cgtcgcaaat tgcgctggtg gcgattccga ttgcgattct
    18241 ggttaacgtg gcgatgttac tgacccgtat gacgcgggtg gtaaatgttg atatctggaa
    18301 tatctggcat atgaccttca ccggcgcgtt gctgcatctg gcaaccggtt catggatgat
    18361 agggatggcg ggtgtggtaa ttcacgcggc gtttgtttat aagctcggcg actggtttgc
    18421 ccgcgatacc cgaaatttct ttgagctgga aggcattgct attccgcacg gtacgtcggc
    18481 gtatatgggg ccgattgcgg tgctggtcga tactatcatc gagaaaatcc caggcgttaa
    18541 ccgaattaaa tttagcgccg acgatattca gcgtaaattt ggtccgtttg gcgagcctgt
    18601 caccgtgggt tttgtgatgg ggctgattat cggcatcctc gcgggttacg atgtcaaagg
    18661 tgtattgcag ctggcggtaa aaacggcggc ggtgatgctg ctgatgccac gggtgattaa
    18721 acccatcatg gatggtttaa cgcccatcgc taagcaggcc cgtagtcgtt tacaggcgaa
    18781 gttcggcggt caggagttcc tgattggcct ggatcccgca ttactgctgg ggcatacggc
    18841 ggtggtatcg gcaagcctga tttttattcc gctcaccatt ttaattgctg tttgtgttcc
    18901 gggtaatcag gtactgccgt ttggcgatct tgccactatc ggcttcttcg tggcgatggc
    18961 ggtcgcggtg catcgtggaa atctgttccg caccttaatc tcgggtgtca tcattatgag
    19021 catcaccctg tggatcgcga cgcaaactat tggtttgcac acccaactgg cggctaatgc
    19081 tggggcgtta aaagccgggg gtatggtggc ttcaatggat cagggcggtt ctcccattac
    19141 ctggttactg attcaggttt tctccccgca aaatattccc ggtttcatta ttatcggcgc
    19201 aatttatctg accggtattt tcatgacctg gcgtagagcg cgtggtttta ctaaacaaga
    19261 gaaagccgtt ctcgcagaat aatttttacc tgagggggag gttatccccc ttaacaatca
    19321 ggagttttta tgaaatcagt agtgaatgat actgatggtg tcgtgcgcgt tgcaaaaagc
    19381 gtcattcctg aaattaaaca tcaggatgag gtgcgggtaa aaattgccag ctcgggctta
    19441 tgtggttccg atttacccag gatatttaaa aatggtgcac attattatcc aataacgtta
    19501 ggccatgaat ttagcggcta tattgatgcg gtaggatccg gtgttgatga tttacaccct
    19561 ggcgatgcgg ttgcctgtgt gccgttatta ccctgtttta cttgtccaga gtgtttgaaa
    19621 gggttttatt cccagtgcgc aaaatatgat tttattggct cgcggcgtga tggtggattt
    19681 gccgaatata ttgtcgttaa ccgaaaaaat gtctttgctc tacccacgga tatgcctatt
    19741 gaggatgggg cttttattga gccgattacc gttggtctgc atgcttttca tttagcgcaa
    19801 ggttgtgaga ataaaaacgt tattattatt ggtgccggaa ccattggcct gctggctatt
    19861 cagtgcgctg tcgcgctggg agcaaagagt gtgacggcta tcgacattag ctcagaaaaa
    19921 ctggcactgg caaaatcttt cggtgcgatg caaacattta acagtagcga aatgagcgcg
    19981 ccgcaaatgc agggcgtttt acgcgagctg cgctttaatc agcttatcct cgagacggct
    20041 ggcgtaccgc aaactgtcga actggcggta gagattgccg gtcctcatgc ccaactggcg
    20101 ctggtgggca cgttgcatca ggatctgcat ttaacatcgg caacgtttgg caaaatattg
    20161 cgtaaagagc tgacggttat cggcagttgg atgaactact ccagcccttg gccggggcag
    20221 gagtgggaaa cggcgagccg gttgctgaca gaacgtaagt taagcctgga gccattaatc
    20281 gctcaccgtg gaagctttga aagcttcgcc caggcggtgc gtgacatcgc tcgtaatgcc
    20341 atgccgggca aagtgttgct cattccctga aaccgcgggc cagcgtgatg ctggcccggt
    20401 attgtgcaaa acagatcatt caccaatggt cccccttcgt ttacactagc cacaattgaa
    20461 tatgggtaaa tgacgatgaa ttcattcgag cgaaggaata agatcatcca attagtgaat
    20521 gaacagggaa ccgtgcttgt tcaggatctg gcgggagtat ttgctgcctc ggaagcgaca
    20581 atccgtgccg atttgcgctt tctcgaacaa aaaggcgtgg ttacgcgctt tcatggcggt
    20641 gcggcgaaaa taatgtccgg taatagtgaa accgagaccc aggaagtcgg gtttaaagag
    20701 cgatttcagc tcgccagcgc gcccaaaaac agaatagcgc aggcggcagt caaaatgatc
    20761 catgaaggga tgaccgttat tctcgacagc ggaagtacca caatgcttat cgccgaagga
    20821 ttaatgaccg ctaaaaatat cacggtgatt accaacagtc tcccggctgc gtttgccctt
    20881 tccgaaaata aagacattac tctggtcgtc tgcggtggca cggtccgcca taaaacgcga
    20941 tctatgcatg gatctattgc tgaacgttcc ctgcaggata ttaatgccga tttaatgttt
    21001 gtcggtgctg atggtattga tgcggttaat ggtattacga cctttaatga aggttattcg
    21061 attagcggtg cgatggtcac cgccgctaat aaagtgattg ccgttctcga ttcatcgaaa
    21121 tttaaccgtc gcggttttaa tcaggtactg ccaatcgaaa aaattgacat tattattacc
    21181 gatgatgctg tatcagaggt ggataaactg gcattgcaga agacacgggt aaaattaatt
    21241 acggtgtagt attgttctgt atgccggata aggtatttac accgcatccg gcatttcgga
    21301 ttctccctgg ttcaagagag aatagtttga ttaacgcaat aatggacaat ctggtggtaa
    21361 tcgacaacgc aacgccgccg gaagtatttc aatatgaaag ttttgtccgc tcaacggttc
    21421 gccatcaaga ttaaaagtga tttcgtgtgg ggcttgtata tcaaaccacg acgaagcgcc
    21481 ttcgataata ttcgggttat cttcgtcaga ttttaaggtt gatacgagag ccggaagaat
    21541 ttcatcgccg gtaaaaatgc gcagttgcag caagccatcg ttaattaacg cgttcggaca
    21601 caattgctga ccgccaccgg cctgacgccc gttaccaata ccaatgacca gggcgtcacc
    21661 ttgccagtga aagttttcac cgcggatttc acaacggtcc ggttgcagag tatccatgcg
    21721 cattaagcca tgaatgatgt aagagacgcc acccagcgcg gcttttaatt tttccggcgt
    21781 ttctgtggta atacgcgtcc caaatccgcc tgtcgccata ttaataaaac aggtttgttt
    21841 gttgacctgc gccatatcta tcgcaatggc gttaccggca attgccagtt tcagcgcctt
    21901 atccagtgcc tcaggaatcc ctacactggt ggcaaaatca ttggcggttc ctaatggcaa
    21961 aattcccagc gcgggtatgt catccccctc acactgaatc aacgccgtag caacttcatt
    22021 aatggtgcca tcgccaccac cggcaatcac cgttgcgacg cccaacttcc gggcctcctc
    22081 tacatatcgt gcggcatcgc ctttctccca ggtgacccgc acatggatcg tcattccttc
    22141 ctcacgcaac agtataattg cttcgcgcaa gggtagattg tcagtacttt tgccattaag
    22201 aatcagtaag ctggcgggaa attctgccat gatcgtatgt gcctttatga ttggtctgta
    22261 gatagtgtag agcagaaaac aaaaggtgga gtcagaacag gatgaaagtc ggaggataaa
    22321 aagtcagttc aagtatggag gattcagtgg ttaccaccag gagcttgcaa tggggacagc
    22381 gaatactaac ggctgccacc gataaatttc aaaaaagagc atatacctaa tattcaacta
    22441 aacagtggca tcttcaatat aatatattaa agcccccatg gagttaccct gaagggcctc
    22501 aatgtccgta attcctactt atgtaggaaa tgttgtacag aacatttatt ataatcctat
    22561 tcaattataa taatcatgcc attattatat ttaaacacta gagagtgtcg ttggtattta
    22621 atgggggaag gtgagatgaa aaagatagct gcaatatcat taattagtat ttttattatg
    22681 tctggttgtg ctgtgcataa tgatgagaca agtatcggta aatttggtct tgcatataaa
    22741 agtaatattc agcgtaaact cgataaccaa tactacaccg aagccgaagc ttctttagcc
    22801 aggggcagaa tatctggtgc agaaaatata gtaaaaaatg atgcagccca tttctgtgtt
    22861 actcagggca aaaaaatgca gatagttgac ctgaagacag aaggtgcagg attacatggc
    22921 gtcgctcgtc tgacattcaa atgtggagag tgagaatatt ttttggtaag cgtcaaacat
    22981 gcgcgttctg gttgtgctta gccggaacct gtgcgagcac gatgacgtta cgtgaaaggc
    23041 atcgtgctat gaagggagat tctatcgatg tggtcaatgg aagacggtga ccagggatag
    23101 ggcttatgca taaaaaataa gcccgtgtaa gggagattac acaggctaag gaggtggttc
    23161 ctggtacagc tagcatttta tgggttatgt ttttcagcga aacggatgat aaccttaata
    23221 aatacagctg tatgtgatcg gtttctaaga attttccatc cgggaaaaat aatcgaaatt
    23281 aatcacttac cgtggggatt acgcgtggtt tccccggaga aattacgcat cagcagcgcg
    23341 taattgagct caagatcctg cgggaccggg agccacacag tataaccatc gcctggtgct
    23401 atcggcatag cttcgccttt ggcgttttcc atgtgctcaa gggtaaaatt aatgttgcct
    23461 tgcggcgtca tcagctcaag gctgtcgcca acggagaatt tatttttcac cgctaccgcc
    23521 gcgaggtccc ccttgcgctc accggtaaac tcaccaacaa actgctggcg gtcagaaact
    23581 gaataaccgt attcgtagtt ctgataatcg tcgtgagtat gacgacgcag gaaaccttcg
    23641 gtatagccac gatgcgccag accttccaga gtttccagca ggctggtatc gaacggtttt
    23701 cccgcagcgg cgtcatcgat agctttgcgg taaacctgtg cggtgcgtgc acaatagtag
    23761 aaagatttgg tacgaccttc gattttcagc gaatgcacgc ccattttggt caggcgttca
    23821 acatgggcga tagcgcgcag atctttcgag ttcatgatgt aagtgccgtg ctcatcttca
    23881 aacgcggtca tatactcgcc cggacgctgg gcttcttcga tcataaacac tttgtcggtt
    23941 ggcgcgccga tacccagcgt cggctcaaca ttttgcaccg gaatcggttc gtacttgtgt
    24001 acgatgttgc caacatcatc ttctttccct tcctggacgt tgtactccca gcggcaggcg
    24061 ttggtgcagg tgccctggtt cgggtcgcgc ttgttgatat agccagagag caggcagcga
    24121 ccggagtagg ccatgcacag cgcgccgtga acgaagatct cgatctccat atccggcacc
    24181 tgattgcgga tctcttcaat ctcttccagc gacagctcgc gagagaggat cacgcgggtc
    24241 aggcccattt gctgccagaa tttcaccgtc gcccagttca cggcgttagc ctgcaccgaa
    24301 aggtggatcg gcatttcagg gaagtgctca cgcaccagca taatcagccc tggatcggac
    24361 ataatcagcg catccggccc catttccacc accggtttca ggtcacggat aaaggttttc
    24421 agcttggcgt tgtgcggtgc aatgttgacc acgacataaa actttttccc cagcgcgtgg
    24481 gcttcattga tgccgagctg aagattttcg tggttgaatt cgttgttgcg cacacgcagg
    24541 gaataacgcg gctggcccgc ataaacagca tctgcgccat aagcgaaagc gtaacgcata
    24601 tttttcagcg ttcccgccgg ggaaaggagt tccggtttaa acataattct ctcgttctga
    24661 tgacaggtca gatccgcttc acctgatgaa gcggttaggg gagtgccccc actttaaggg
    24721 cgggcattgt agcgcaatcg gcgggtttag ctccagtgta aatgtctggt ggcgtcctgg
    24781 ttttggttaa ggatacgaat gacgataatt tcccctggtg attgcaggaa ataaatgacg
    24841 tggcgttcaa aaggaagaac aaaaatacct tcgcctaatt caggtctggg cgtacctata
    24901 ttattgttac tcagaatctg gagaacgtct gaaagatgtt ccacatatct gtcggcttgt
    24961 ggttcgccga aatggtgata gctgtaatac cagatgcctt ctaaatcctc attggctttt
    25021 ggcatgagtt taataatacg cacattattt ccttatccct gctttcacct tccgtaagaa
    25081 cgcgtccttt tcccacggct gagcttcacc actactcaac ccttctgcga gcatgtcacg
    25141 taacgcttgc aggcgagact ctgcttgctt ttctcgaagt aatcgcagtg actcacggat
    25201 cacttcgctt tgggtgcgat agtcgccgga ttcaattaag gactcgataa attcacgcaa
    25261 ctcatcgccg agatcaaccg tcatcgttct ggccatttta cgcccgcctg taagtaaaaa
    25321 tcttacatca gtgtatgtca taaaccggat agcgtcacga aagtattaga agatattttg
    25381 gcacatgaac atgttcagga ggataagtat gggatggtaa acaaagaaag cacaacgagt
    25441 gctcgtcagt gcccactatt ttaatggcgg cggttactct taattattaa taactaagcg
    25501 ttatttggat ataaagccgg acgccaacaa cccacgcccg gcatcgtaat tacgtattgt
    25561 ctttcaccgt ctggcttgtg ccattggctt taacggaggc aattccggtt tcacgagatt
    25621 gcgcggtggc gtacatctgg ctggagccga taatttgatg attagcggct ttcagattga
    25681 aatagaattt gccgttactt gccgtttttt tctcatagcg ttcttcttgc gggctgttgc
    25741 tacgcaccga cgcgatgccc ttttccgcag aggcttttga ggtatacagc tcgctggtga
    25801 ggatagtctc accattgccc gcttttagca caaaccggaa ctgattatca ctgctcttac
    25861 tgagttcaaa ccaaccagcc ataatgactc cttgattgaa attgtgtatc gaaaacagca
    25921 tctaaaggtg ctgtttaccg attaaagtat gagcgatttt ctgatgcctg ccagggcgga
    25981 gagataatgt cagtttgctg accatgcgtg ggataatgtt gccggatctg gtctgaacgc
    26041 catctccggc taacaaagtc atgtcgctaa aactaaacga tgcggcaggc gtcggcttcc
    26101 cagcggtaac caacgccgta aacggcgcgg ataaatgact gttcggcgtc gagagattcc
    26161 agcttgcggc gcaggttttt aatgtggctg tcaatggtgc ggtcggttac tacgcggtag
    26221 tcgtcataaa gatgattgag caattgctcg cgggagaaca cttttcctgg ttcatgagag
    26281 agcgttttca gcagacgaaa ttccgcaggc gtcaggtcaa gcattttaac gcgccatgaa
    26341 gcctgaaaac gaccttcgtc gataatcaac gggctttcag catcctgttg ctgcaactca
    26401 cgctgcggtt tgcaacggcg caaaatggtt ttgacgcgcg ctaccacttc ccgtgggctg
    26461 tacggcttgc agatgtagtc atctgcgcca atctccagtc cgagcaggcg gtcgatctct
    26521 tcgatttttg ccgtcaccat cacgatagga atgtcagaaa aacgacgaat ttcccggcac
    26581 agcgtcaagc catcggtgcc agggagcatc agatccaaca ggatcagatc cggtggtgtc
    26641 tggcgcacat acgccagtac ctgatcgccg tggctgataa gcgtcggcgc atagctcgca
    26701 gcacgcagat aatcaatgag caactgcccc agcttcggtt catcttccac gatcaaaata
    26761 cgcggtgtgt tttcgtcgat tggtaactcg gtcatacttc tctctgtaaa tcccgttcca
    26821 gcggtaactc tactgtaatg cttaccccgc caaaaggcga atgggcagca ataatgcggc
    26881 cattatgtgc ttcaacaatg ttcaggcaaa tcgccagccc cagcccggaa ccgccgctgg
    26941 cacggttgcg ggaaccttcg gtgcgataaa aacgttcaaa caatttttgt agctgatcgt
    27001 cactgacgcc cggtgcgctg tcggcaaagg tcaggcgcac cgttttgtcg tgctgctcgg
    27061 cagagatttt caggctgccg ccgctgtcag tgtagcgcag gctgttttca agtaagttat
    27121 tgaataactg cattaaacgg tcgcgatcgc caaatacggt aatactgtct ggcagggaaa
    27181 attgcagttt caggccacgg ctggcaaatc gttcgcgaaa tgcgccgccc gccacttcca
    27241 gcagtgggat caaatctacc ggtgcttttt gataggcgag agcgccttca tcagacatcg
    27301 acaactgatg gagatcgtca accagtttgg tcagtgtacc gacctccgcc tgtaaagacg
    27361 ccaccgtctc cggcgtgaat ttacgcacgc catcctgaat ggcttccagt tcaccgcgca
    27421 gcaccgctaa tggcgtacgc agttcgtgag aaatatcggc cataaaatcg cgccgcattt
    27481 gctggttttt ctccagcgtg ctggcaagct ggttgaagtc ttgcgccagc ttgcccagtt
    27541 catcttcact ggtgggcgtt acgcgggtag tgaaatcgcc cgccgccagt ttgtgcgtgc
    27601 catcgacaag tcgttttacc ggtgccagta aaccgcgcgc cagcagaaaa gtggcaagtg
    27661 ccgcgagtaa cgttgccagg gcgacaatca accagctggt ttgccgctgt tgtttatcga
    27721 aattgatatc agtattgcgc gttaaccgct caacggggga ggcgatcacc gcgccaactt
    27781 ccgcaccgtt gaccagaatg ggtcgccgtg taccgtcagg tggaatcggc gctcgcggac
    27841 caaccagcac tttgttgttt tgatcaacca cccagaactg ggtacgccag ccgtgcggtg
    27901 gcatacccgg gccgggttta tcttccgaat tatcgtgttc aaatgaacgc aggatctgaa
    27961 agacaaagcg atcattgttg cgcaggaagc gccagttgcc atgctgcgca tactgctcgc
    28021 caagcgcatc acttaacagt tgtaatcgct gttcattacc atgcttgatg taatcaataa
    28081 agccacgctc aaaactgata cgcaccgccc agtgcatact gatcagcaag acaatgcagg
    28141 tggcgaaaat cgccagaaac agtttgccgg taataccggg tcgccagaac ttcattgcgc
    28201 gctccttttt cgccgcgaaa tagctacatt ttgatgcgta tcgttcggca ctctggcaaa
    28261 gatgaacgcc ggaagggcga tgatcaacgc catgctaagc caggtgtaca taaagacggt
    28321 ttgtgtggtg ccgctgtcga cgctgacatg ctgtgaacca aaaagtccca gcaacagccc
    28381 ggcgatagtg acgccgatac tcatcgacaa ttgcataatc atcgacagca ggctgttgcc
    28441 gctgctcgcc agattgtccg ggagatcttt cagcgtcagg gtgttcatgg aggagaaacg
    28501 cgtcgagttg accatccctt gtaaaaacag gacgaacggc aaaacgtagt accagcccag
    28561 cagggcggta gtcataaaca acagggtgac cagcgacaga cccagcgtgg tcgctaccag
    28621 tacccgacga taaccaaagc gattcaccac ctgtaccaca attcgcttca tccccatgct
    28681 gccgagcacc atcgggatca tcatcagtcc ggcatgaaaa ggcgagaaac cgaggccaat
    28741 ttgcaggaaa accggtgtca taaagggcaa catgccactg ccaatacgtc cggcaaagct
    28801 ccccgccagg cccagcgaaa aggtacgagt gcggaacagt ttcagactga aaagagcgcg
    28861 gtggttattt ctggcgtgca gcagataaag caccagtgcc accacgccaa ctgcgaccag
    28921 gcctgcaatc gtcagcggcg ataaacctgt acctttactg ccgtccagcg ccagggttaa
    28981 taccgccatg ccaaccgcca gcaataaaaa tccggagaga tcaaagcgcc gcgtctgcat
    29041 ggtgtagttc ggcattaaca gcaatgtggc gatcgcaccg ataatcccca ccggaatgtt
    29101 gatcaaaaag atccagtgcc acgacgcgta ctccaccagc agaccgccga gcgccggacc
    29161 gagcagcgga ccgacctgac cgggtaacgt gacaaaggtc atcgccgcca tatattgctc
    29221 gcgcggtacg attttcatca ccgtcaatct gccgaccggc accatcatcg cgccgccaac
    29281 gccctgtaac gcgcgtgcca gcaacagttc gttcagcgtg ccggaaagcg cgcaaaacag
    29341 tgaaccgaga gtaaacagca cgatggcggt aaagaaaata ttgcgcacgc cgactttgtc
    29401 cgccagccag ccgctggcgg gcagcatcac cgccacggtc agcacataag agacaatgac
    29461 catgtgcata tgcaacggac tttccccgag gctttgcgcc attgagggaa gggcggtgtt
    29521 tacgatggtg gtgtccagcg actgcataaa gaagccgaaa gccacaatcc acaattgcca
    29581 acgggtgctg tcgggaagat ctgtcattta ctcggttacc gtttgtttag gtttacgcga
    29641 aaaacgcagc cgcagacggt cgaaaaagag atacaccacc ggcgtggtat acagcgtaag
    29701 gagctggctc attaccagtc cgccgacaat ggtgatcccc aggggttgcc gcagctccga
    29761 gccgtcgccg cccgacaaca ccagcggcag cgcgccaaac agcgccgcca gggtagtcat
    29821 cataatcggg cgaaaacgca gcagacaggc ctggaaaata gcttcctgcg gcgtcaggtt
    29881 accgtgccgt tgggcttcaa gcgcaaaatc gaccatcata atggcgtttt tcttcacgat
    29941 gccgattaat agcatgatcc ctatcagggc gattaggctg aacggggcat tgaacagctc
    30001 cagcgccagc agcgctccaa cacccgccga gggcagggtg gagagaatcg tcagcgggtg
    30061 cacataactc tcgtaaagga tacccagcac gatatacacc gtggcgatgg cggcgataat
    30121 caggatcacc tgtgagttca tcgtctcctg gaacacctgc gctgttccgg caaaactgcc
    30181 gcgcaccgtc gaaggcacac caagctgggt cattgcgcga tcgatcgccg cactggcgtc
    30241 cgagagcgat tttccggtcg gcaggttgaa cgaaatggtc gaggccgccg ataatccctg
    30301 atgattcacc gatagtgggg cattcgccgg ttgccattta gcgaaatacg acagcgggat
    30361 cgcttttcct tcgttattga taacgaacat tttttccagc gcgctgatgt cctgggtata
    30421 gcgcggatcc acttccatca ccactttata ctggttcatc ggctggtaaa tggtcgagat
    30481 ttgccgctga ccgaaggcgt tatttaacag gttgttggcg gcttgtacgt cgattcccag
    30541 ccgtgccatg gtgtcgcggt cgtaaaccag attcatctcc gcgccgttat cctgctgatc
    30601 ggagttcacg tccgccagtt ccggcaacgt cgccagtttt ttgcggattt tcggctccca
    30661 ttctcgcagt gccgccaggt cgtcggataa caacgtgtac tggtagctgg cgttcgactg
    30721 acgcccacca acgcgaatat cctgtaccgc catcaggaac agattcgccc ccggttcttt
    30781 cgccagtttt acgcgcagac ggtcgataat ttgctgcgcc gtttcgctgc gttcgtcgcg
    30841 tggcttgagg gtgataaaca tcatcccgct gttcactcgc gaaccgcctg taaagccggt
    30901 gacattatcc actgccggat cgtcacggat aattttcatg aaatcctgca acttaccgcg
    30961 catcgcctga aacgaaatac tctgatccgc ctgaatcccg cccatcaaca cgccagtgtc
    31021 ctgctccggg aagaaggttt tcgggatcga gatatacagc cagatattca gcgcaatggt
    31081 gccaagcagc accacgccca ccagacgggt atgattgagc acccatttca gtgacttgcc
    31141 gtagccttgt tgcagggcta ccaacatgcg accaaaacca cgcagtcgct tttgctcgca
    31201 cggcttgctg gctttcagca tccagccaca catcattggc gttaatgtca gagaaaccag
    31261 caacgatata ccaatggcga cagaaagcgt cacggcaaat tcgcgtaaca gtcggcccgg
    31321 caatccgccc atcaacagca gcggcaggaa caccgccacc agtgacagac tcatcgacag
    31381 caccgtaaaa ccgacttcgc gagtaccttg cagtgcggct tgcaacggtt tcattcccgc
    31441 ttccagatga cgggcaatgt tttccagcac cacgatggcg tcatccacca cgaaaccagt
    31501 agcgatggtg agcgccatta acgaaagatt attgagactg aaaccgcaca ggtacatcgc
    31561 cgcaaacgta ccaatcagcg aaaccggcac cgcaacggcg ggaataatag ttgcgcgacc
    31621 cgagcgcagg aataaaaaca ccaccagaat caccagcgcc accgagataa tcagcgtttg
    31681 ctcgacttct tccagcgagg cgcgaatggt gggggagcga tcctgagcaa tttgcagatc
    31741 aatcgccgcc ggaatggttt cctgcaactc cggtaatttt gcccggatgc tgtcaaccgt
    31801 ctggataata ttggcttccg gcagcttgcg gatcatcagc aaaatagccg gtttggcatt
    31861 ggtcatcccg gcgttacgta catcctgtat agagtcggtc actgtcgcca catcgcccag
    31921 acgaaccgcg ccgccgttgt tgtaatgaat aatcagcggc tgatattcgg cggcggtttt
    31981 tagctcatca ttggtctgga tctgccagcg gtgagtgcca tcttccagcg ccccctgcgg
    32041 tttacgcacg ttggcattgc tgatggcggt gcgtacgtcg tccagcgaga cgccctgatt
    32101 aaacagcgcc tgcggattca gcccgacgcg tacggcgggc agtgagctgc cgccgacatc
    32161 gacatcacca acaccgtcga tttgcgaaat cgtcggagcc agctgcgtcg aggcaaaatc
    32221 gtacagttca ccctgcgaat aagtatcgga tgtcagcgtg aggatcataa ttggcgcatc
    32281 cgacgggtta gctttgcgat aggtcggacg gctgggcatc ccgctgggca gcaaactttg
    32341 tgcagcgttg atcgccgcct gcacatcgcg cgccgcgccg ttgatatccc ggtcaaaatc
    32401 aaactgcaaa ataatacgcg tgctgccgag cgaactgctg gaggtcattt cactgactcc
    32461 ggcaatgcgc ccaagtgagc gctccagcgg cgtggcaacg gaagacgcca ttgtttctgg
    32521 tgacgcaccg ggcagcgagg cgctgaccat aatcaccgga aaatcgacct gcggcagcgg
    32581 ggcgaccggc agcatacgga agcccagtat gccgcacagg gtaatggcaa ccgacagtaa
    32641 aatcgtcgcc accgggcggt aaatgaagag ggcaaaaaac ttcacttacg cctcctcttc
    32701 atgacgggca aagcggcttt tggtccacaa tgccaggcgg tcgaacagca aataaatcac
    32761 cggcgtggta aacagcgtca gcacctggct gacaatcaga ccgccgacca tgccgatacc
    32821 taacggacga cgcagttccg cgccgacccc ggtactcaac atcagcggca gcgcgccaag
    32881 cagagccgcc agagtggtca tcaggatcgg acgaaaacgc aacagacaag cctggtagat
    32941 tgcctcgcgc ggcgacatgc cttgctcgcg ctcggcagcc agcgcgaagt cgatcatcat
    33001 gatggcgttc ttcttcacga taccgatcag caaaataatg ccgataatcg caatcacatc
    33061 cagttcgcta ccagcaatca gcaacgccag cagtgcgcca acccctgcgg tgggtagcgt
    33121 cgagagaatg gtgatcgggt gaataaagct ctcgtacaga atgccgagca cgatatacat
    33181 cgccaccacc gccgcgacaa tcagccagac agtgctgccc agcgccgact ggaaagcgag
    33241 ggtgctgccc tggaactgcg tggtgatatc caccggcaga ttcagcgtct tttcggtgtc
    33301 cataatcgcc tgcaccgcat cgcccagcga atagttatcc ggcacgttaa aggagatggt
    33361 cgttaccggg aactgatcca gatggttgat ggagagcggc gcaaaacgct gctcaatttt
    33421 ggcaattgag cttagcggca ccacgccgcc gtcgctgctg gtcaggcgaa tggtatccag
    33481 cgccgcgagg cctggggtat tttcggtgtt gtgctccagc accacgcgat actggttggc
    33541 ctgagtataa atagtggaaa tcagccgctg accaaacgcg ttgtacaggg cgttatcgac
    33601 atccgccatg ctgatcccca gacggctggc gctgtcgcga tcaacattga catacgccac
    33661 cagccctttg tcctgccagt cgctggagac atcagaaagc tgtggcagtt gctggagttt
    33721 ttccatcaac tgtggcaccc aggtactgag cgcatccagt gaggtggcct gcaaggtaaa
    33781 ctggtactgg gtgcggctga cctgagtatc aatagtcaga tcctgcgttg gttgcaggaa
    33841 gagatcgacg cccggcactt tatctaccgc cgtttgcaga cgggcgatga ctttttgcac
    33901 ccgatcatca cgttcatcca acggtttgag gttgatttgt aaacgtgcac tgttcagcga
    33961 cgggttagtg ccatcaacgc caacaaatga ggtcaggctt tgcactgccg gatcctgcaa
    34021 aatcacgtcc gcgacctggc gttgtcgctg ggccatattg gcaaaggagc tggattgcgg
    34081 tgcctgcaaa gtgccctgaa taatgccatt gtcctgtacc gggaagaaac ctttcggaat
    34141 gaacacccac agcagcacgc taagcagcag cgtgctgagt gccacgctta aggtcagcca
    34201 cggatgattc agcactttcg ccagtccacg accataggcg gcgattatcc tgtcgaacat
    34261 tttttccgag gcacgggaga agcggttctg tttacgcaac gactcctggc tgagcatccg
    34321 cgcgcacatc atcggtgtca gggtcagcga caccaccgct gagatcaaaa tcgctaccgc
    34381 cagcgtaata gcaaattcgc ggaacagtcg cccgacgata tcgcccataa acagcagtgg
    34441 gatcaacacc gcaatcagtg agaaggtcag cgagataatg gtaaagccga tttcacctgc
    34501 gcccttgagc gccgccgcca acggtttttc gcctttttcg atatagcggg aaatgttttc
    34561 gatcaccacg atggcgtcat cgaccacgaa tccggtggcg atagttaacg ccatcagtgt
    34621 caggttattg attgaaaaat cgagaaacac cataaccgcg aaagtgccga ttaacgacag
    34681 cggtacagca acaccgggaa tgatggtcgc cggaatattg cgcaaaaaca ggtagataat
    34741 catgactacc agcgcgatag ccatcatcaa ttcaaactga gtatcatcga cggatgcgcg
    34801 gatattggtg gtgcgatcgg aaagcactgt caccttcacc gatttcggca gactctcagt
    34861 gagctgtggc agcatctgcc gaatgctgtc ggcggtggag ataatgttag caccgggctg
    34921 gcgctgaaca ttcatcacaa tggcctgttc tttgttcgcc cacgcgccga gccagctgtt
    34981 ttctgcacct tgctctacag ttgcgacatc gcccagacga attggcgcgc cgttctggta
    35041 ggcgatgatt agctggcgat actcttcggc ggattgcatc tggtcgttcg cggaaagcgt
    35101 gaccgcacgg gaagggccgt cgaggctacc ttttgccgag ttaacgttag cgccggtaat
    35161 ggcggtgcgc acggtttcgc tggtcaggcc gagggcggca atcgcctgag cgttaagttt
    35221 gacgcgaaca gccggacgct gaccgccgga aagcgtcacc aggccgacgc cggaaatctg
    35281 cgagattttc tgcgcgacgc gggtttccac catatcttct acctgggtca tcggcatggc
    35341 ggttgaggtg acggcgagcg tcatgatcgg cggatctgcc gggttcactt tgctgtaaac
    35401 cggcgggtta ggcagatcgc tcggcaacaa gttggtcgca gcgttaatcg cggcctgcac
    35461 ttcctgctcg gcgacatcga gcggtaatgt tagctggaac tgcaaagtga taactgacgc
    35521 accgccggaa ctttgcgacg acatctgttt caggccagac atctgcccga actggcgttc
    35581 gagcggcgcg gtaacggcag aggtcatcac atccgggctg gcgccagggt agagtgtaac
    35641 cacctgaata gtcggataat ccacttccgg cagtgccgaa acgggcaggg cgcgataacc
    35701 gataattccg gcgagtaaga tcgccaccat cagcagcgtg gtggcgacag gacgcataat
    35761 aaacaggcgc gacgggccgc ctgtgctgct cgggggtaac acctgcatca ggagcgtgct
    35821 ccttttttcg cgtattcgcg gctggtggct ttctcttccg aagtggtggt gctctgggct
    35881 tccaccactt ccactttcgc cccttcggtc aggcgatcaa tgccgtctgt caccacgcga
    35941 tcgcccgcag aaatacctgc acggatcacc actttctgac tgtcctgaat gcccggcgtc
    36001 accagatgtt tgctgacctt gttttcgcta ttcagcaccc agacaaaatg gccttcattg
    36061 cccatttgca gggcggctgt tgggatcact acggcgtttt gttcggtgtc gactaacatg
    36121 cgcgcgttaa caaactgatt gggaaacagc gcatcatcct gattattaaa gcgtgctttc
    36181 actttaatcg taccggtagt ggcatcgatt tggttatcga gacttaacag cgtgccttcg
    36241 cttaatttct ttgaattggt gcgatcccag gcttctacca ccagcggttt cccggctttt
    36301 tgcgcctgca cgacggtagc gatatcgctt tccggcaggg taaagactaa atcgatagga
    36361 tgcgtctggg tgatcaccac aattccggtg gtatcaccac tggagatttg gttaccaaca
    36421 tcaacctgct tgagaccaac gcgaccatcg actggtgcgg tgatgcggct ccagtcgagt
    36481 tgcagctgcg cgctggcgac gcttgcttca tcagccttaa tggtgccttc ggtttcactg
    36541 accagtgcct gttgggcatc cagctcctga cgggatacga gattggtttt tgccagttgc
    36601 tgataacgcg ccagatcacg gcgggcgtta gcaagcgtgg ctttatcttt tgccagttgg
    36661 ccctgggctt gtgctaatgc aactttgaac tggctggggt caatttctgc cagtaaatcg
    36721 cctgctttga cctgctggcc ttcctggaaa tgtaacgcca tcagttgacc gtccacgcgg
    36781 ctgcgcaccg taacagtatt agcggcggta atggtgccaa gcccggtgag gtaacgcgga
    36841 actgcctgtt ctacggcggt cgccgcctga accggggcta atgggccgga acgcatacca
    36901 cggcgaccac ccgctggcga ttgctgcgct tgtttcgtcg cccctggggc tgcactctgg
    36961 gagtcattgc ggccttgcca gaaccagaat gcggcgatgg cggcgataac caccacgatt
    37021 acgattaccc aacgggattt ataactgcct ttcatcgtta agagtttctc ttcctgaaac
    37081 gtttcgcgga atgatactag tgtagccagc caataaggga gaaaaatgga ggaattatga
    37141 aacactatcg ggaatcgtct gaatggcggg cacatttgcg agcacgcatc cagtaataac
    37201 acaggaaact attttatcta cgcgttagcg atagactgca tacagggcga aaggaggtaa
    37261 gccgcagatt tcagccggat gctgaaacgg gaaagcccct cccgaggaag gggccttaaa
    37321 taaggaaagg gtcatgatga agctactcat catcgtggtg ctcttagtga ttagctaccc
    37381 agcatactaa gactaccagg gcgggggaaa ccccgctcta ccctcactcc tgaaagtatg
    37441 ccttcacgat aagattgtca atccgcaggc tttgccgtct gcgatcctgc cagcaaaaat
    37501 tctttgcgag acgttacgca ataatcacac tggaaactat tttatctgca agtcagcgat
    37561 agactaagtg catggcgaaa ggaggtaagc cgacgatttc agcgggacgc tgaaacggga
    37621 aagcccctcc cgaggaaggg gccttaaata aggaaagggt catgatgaag ctactcatca
    37681 tcgtggtgct cttagtcata agcttccccg cttactaaga ctaccagggc gggggaaacc
    37741 ccgctctacc ctcactcctg aaagtatgcc ttcacgataa gattgtcaat ccgcaggctt
    37801 tgtagtctgc gatcctgcca gcaaatattc tttgcgagtc gttacgcaat aatcacagag
    37861 gaaactattt tattcacgcg ttagcgatag actgcattca gggcgaaagg aggtaagccg
    37921 atgatttcag cgggacgctg aaacgggaaa gcctctcccg gagaagaggg cttttaataa
    37981 ggaaagggtt atgatgaagc acgtcatcat actggtgata ctcttagtga ttagcttcca
    38041 ggcttactaa gaacaccagg gggaggggga aacctctccc taaccctcac tcctgaaatt
    38101 atgtcactcc gcccttagtg tcaatcgacg gctctccgtt cttgcgaggc gaatagcaaa
    38161 attttctgcg ccgccgggcc taattctgaa agtttcttcg tttgctctta tttcattatt
    38221 gcagtcggta tgataccgga cctcaataat tctctaagaa aggatttctt atgagcgaac
    38281 aaatcacgtt tgccaccagc gattttgcca gtaacccgga accacgttgc ccctgcattt
    38341 tgctgctgga tgtttctggc tcgatgagcg gcagacccat taatgaactt aacgccggat
    38401 tggttacctt tcgcgatgag ctgcttgccg attcgctggc cttaaaaaga gtggaactcg
    38461 gtattgtgac gttcggcccg gtgcatgtgg aacaaccctt taccagcgcc gctaactttt
    38521 tcccgcccat cctgtttgcc cagggggata cgccaatggg ggccgccatt accaaagccc
    38581 tggatatggt tgaagagagg aaacgtgagt accgcgcaaa tggtatttcg tattatcgcc
    38641 cgtggatttt cctgatcacc gatggcgctc caaccgatga gtggcaggct gccgccaaca
    38701 aagtgtttca gggggaagaa gataagaagt ttgccttctt ttccatcgct gttcaggggg
    38761 cggatatgaa gacgctggca caaatcagcg ttcgtcagcc tttgcctctg caaggattac
    38821 aattccgtga actgtttagc tggttatcca gttcgctgcg ttcggtttcc cgctccacgc
    38881 cgggaacgga agttgtgctg gaagcgccaa aaggctggac gtcagtgtga gctggcgtct
    38941 ggtttatgca tccgctgttg gaacgtcgca cattagcgct gatttacctt gtcaggatgc
    39001 ctgccagatg caggttgcct ggttaaacga tcagcaacca ttactggtga tgtttcttgc
    39061 tgatggtgca ggcagcgtct cgcagggtgg cgaaggcgcg atgctcgccg tcaatgaagc
    39121 gatggcttat atgtcgcaaa aagtgcaggg tggggaattg gggcttaatg atatcctcgc
    39181 cacggatatt gtgctgacta ttcgtcagcg actttttgcc gaggcagagg ccaaagaatt
    39241 agcggttcgc gattttgcct gcacgtttct gggattgatt tcatcggcta atggcacctt
    39301 gattatgcag attggcgatg gcggcgtggt ggtggatttt ggtcacggtc tgcaattgcc
    39361 gcttacgccg atggtgggtg aatatgccaa tatgacgcac ttcattaccg atgaagatgc
    39421 cgtttccaga ctggaaactt tcaccagcac tgagcgtgtg cataaagttg cagcatttac
    39481 ggatggtatc cagcgactgg cgttaaatat gttggataat tctccgcatg tgcctttctt
    39541 taccccgttt ttcaatggac tggcgtcagc aacgcaggaa caactcgatt tattacctga
    39601 attgttaaag cagtttttgt ccagcccagc ggtaaacgag cgtaccgatg atgataaaac
    39661 gttagcactg gcgctgtggg cagagtgagt ttctcatggc ggttgaagac aacaattcaa
    39721 ccgccagttt aaacaataaa tataacggct gattagcgaa ctaattttgc tctaagctcg
    39781 tcggcttctt cttttggata ggtgaccaat aaaggatcaa tggtataacc gccgtaaatt
    39841 ttatccccgc gcgtatacat ccagtctgaa atatcttctt tcttgatttt ccactcctgg
    39901 ccatattcta cgtttgtaac aataccaggg tcattgctga ttacgccaat aaacatgccg
    39961 ttagaataag tgacatccgt gagccagaaa tgctcggttc ttccgtgatc ggttatcggg
    40021 gctttgactg cgaaagtatc ggcatctttt ttattcataa cctcgatgaa tttatcggtt
    40081 tctttccgcg catgtttaat cgcgtcagac atcgcttgtt tatcataacc tcctgtcact
    40141 aacgtatccg aaacttcact aaagcccgcg gtagtaaaaa atagcaggct caacatgagt
    40201 aatatctttt tcattttttc tccagaatct ctgtgtagta aaagttagca aaacctgtgg
    40261 aagtgaatca ttatcaggtt ggaattgaat tgtatcctga tgaaattaca ttatcatttt
    40321 tattcttgag tgggctcttc ccatatagaa ataattataa tgggcagtgt tattattcat
    40381 tgaaatataa tattgctgcc cttgaaacat tatgaagaca aatataaaag tatttacatc
    40441 aacaggtgaa atgaccactc tcggtcttga actgggcaaa ggcggtgaag gcgcggttta
    40501 tgatatcaat gagtttgtcg atagcgtcgc caagatttat cacacgccgc cacccgcctt
    40561 aaaacaggac aaacttgcct ttatggctgc gacagctgac gcgcaattgt tgaattatgt
    40621 cgcctggccg caggcaacgc ttcacggtgg acgaggcgga aaagttatcg gttttatgat
    40681 gccaaaagtt tctggtaaag aaccgattca tatgatctat agcccggcac atcgtcgtca
    40741 acgttaccct cattgtgcgt gggattttct actctatgtt gcgcgcaata ttgcttcatc
    40801 ttttgctacg gttcacgagc acgggcacgt cgtgggtgac gtaaaccaga acagttttat
    40861 ggtaggtcgc gacagcaaag tggtactgat tgatagcgac tcctttcaga ttaacgccaa
    40921 tggcacactg catttatgcg aagtcggcgt gtcgcatttt acgccgccag agctacaaac
    40981 cttgtcgtca tttgtcggct ttgaacgcac aaaaaatcac gataattttg gccttgcgtt
    41041 gctgattttt cacgtcttgt ttggtggccg gcatccttat tctggtgtgc cgcttatctc
    41101 tgatgcgggt aatgcgctgg agacggatat tacccatttc cgttatgcct acgcgtcaga
    41161 taatcagcga cgtggtttaa aaccgccgcc acgatcgatt ccgctatcga tgttaccgag
    41221 tgatgttgaa gccatgtttc agcaggcatt cacggaaagt ggcgtggcaa ccgggcgtcc
    41281 gacggctaaa gcgtgggtag cggcacttga ttctctgcgc caacagttaa agaaatgtcc
    41341 cgtttcggca atgcatgttt atcccgctca tttgaccgac tgcccgtggt gtacgctgga
    41401 taatcaaggc gttatctatt ttattgatct cggcgaagag gtcattacca ccggcggtga
    41461 ttttgtgctg gcgaaagtct gggcgatggt gatggcgtca gtagcaccgc cagcattgca
    41521 actgccatta cccgatcatt tccaaccgac tggcaggccg cttcctttag gcctgttacg
    41581 gcgcgaatac atcattctgc ttgagatcgc actgtcagca ttatcgctgc tgctttgcgg
    41641 ccttcaggca gaaccgcgtt atattatttt ggttcctgtg ctggcggcta tctggattat
    41701 tggcagtctg acaagcaaag cttataaagc agaaatccag caacgccgtg aggcatttaa
    41761 tcgcgcgaaa atggactatg accatttagt cagccagagc caacagttgg gcgggctgga
    41821 aggttttatc gccaaacggg cgatgctcga aaaaatgaag gacgaaattc tcgggttacc
    41881 ggaagaagag aaacgcgctc tggcagcact tcatgacact gcaagggaac ggcagaagca
    41941 gaagtttctg gagggatttt ttattgatgt tgcctctatt cccggcgttg gccctgcgcg
    42001 taaagcggcg ttacggtctt ttggtattga aacagccgca gatgttaccc gtcgtggcgt
    42061 taagcaagtg aaagggtttg gcgaccatct gacccaggcg gttatcgact ggaaagcgag
    42121 ctgtgaacgc cgttttgtgt tcaggccgaa tgaagcgata acgccagcag acagacaagc
    42181 ggtaatggcg aaaatgaccg ccaaacgaca tcggctggaa tcgacgttga ctgtcggcgc
    42241 gacagagttg cagcgattcc gccttcatgc tccagcacgg accatgccgt tgatggaacc
    42301 tttacgtcag gcggcagaaa aactggctca ggcgcaggca gatttaagcc gctgctgagc
    42361 cattttcttt taacgaaaca ccacttccgc ccagcgtgcc agcccggcgg tgacggagcc
    42421 aaagtcatcg ccgcctgcaa tcggaattcc cggcaactgt tctgccagcg cttttttaat
    42481 cagcggcgaa cgggcgctac cgccggtcag gtagataacg tccggttttt cctgggcgtt
    42541 atccagggcc agttgcacct gttccagaat ccgcgccagt ggctggctga gggcgctttc
    42601 cagcccttgc tggctaatca gcgtagccag ttcatcgctg ataaacggca gtgaggcgcg
    42661 ggtttccgct acgcttgaaa gagcaatttt gcactcttct gcgctacgca ccaggcgata
    42721 gcttaaacgc tgacgccaga ctttctgtag cagggccact ttttccggtt cgcgggcatc
    42781 gcgtaccaga tcgttaagca gacgaccgtt ggcactactg tagaaatcac tctgcgcagg
    42841 tacgtcgttg atggcaaccg cattccacca cggcagaatc ggcagggcga tgcctttttc
    42901 ggtttcgcca cccatgccca gtaacggcat caggttttta aacgccagtg cgatgtccag
    42961 atcgttaccg ccaatacggc aaccactgtg acccagcagg ctggcttcac gatcgagacg
    43021 cgaacgccac tgcggcccca tcagcagcaa tgaacagtca gtcgtaccac cgccgatatc
    43081 caccaccagc acccgttttt cttcctgcaa ggtggcttcg taatccagcc cagccgcgac
    43141 cggctcgtac tggaatacca cgtccctgaa tccggcacgc ttcgccgcgc gttccagaat
    43201 cccttgcgcc tgggcgtttg cttcatcgcc gcccagcccc tggaagttga tcgggcgacc
    43261 aatcaccgcc tgagtaatcg cttctggcag ctgcgcctgc gcctgctggc gaatgtgcaa
    43321 catcattgcg cagaccagat cctcaaacag cgctacctgc tgcggtttta agccgctggc
    43381 accgaggaac gattttggtg atttcacaaa ccacacttct tctggatcat caatgtactg
    43441 tgccagtgag gaaagaccga actgcacgct tttcgccgta acatcgatat cttcttcgcg
    43501 gttataacga atcgcccgac gtaacagcgc ctgcgtttca tcgtcgtctg ccggaacatc
    43561 atgatggcgg tacagccatt cacttactgc ttcacgcgtt ggcgcgcaaa gcattgaagg
    43621 cagcagcgtg ctgtcgtttt ccattttcag taattgcggt ttaccgtcac gcatgaccgc
    43681 cactgaacag tttgctgtac cgtaatcaaa accaataaac acgaattaat ccccatgccg
    43741 gtgaagaagg ggcgtgactt tagcgaaatg ttgccgtcgc gacaaccgga atatgaaagc
    43801 aaagcgtagc gtctgaataa cgtttatgct gaaagcggat gaataaggag atgcgatgta
    43861 taccctgaac tggcagccgc cgtatgactg gtcgtggatg ttgggatttc tcgccgcccg
    43921 tgcggtgagt ggtgtggaaa cggtcgctga ggattattat gcccgtagtc tggcggtggg
    43981 cgaatatcgc ggcgtggtga ctgctattcc ggatatagcc cgccatactc tgcacataaa
    44041 tttaagtgca ggtttagaac ccgttgccgc tgagtgtctg gcgaaaatga gccgcctgtt
    44101 tgatctgcaa tgtaacccgc agattgttaa cggtgcgttg ggcaagttag gcgcggcgcg
    44161 gcccggattg cgtttacccg gctgtattga tgcttttgag cagggtgtgc gggcgatttt
    44221 aggccaactg gtgagcgtgg cgatggcggc aaaattgact gccaaagttg tacagcttta
    44281 tggcgaacgg ctggatgatt ttccggagta tatctgcttc cccacgcctc agcggctggc
    44341 agcagcagac ccgcaggcat taaaagcgtt aggcatgccg ttgaaacggg cagaggcact
    44401 gattcatctg gcaaatgcgg cgctggaggg cagcttacca atgacaattc cgggcgatgt
    44461 ggagcaggcg atgaaaacgc tgcaaacttt tccgggtatc gggcgctgga cggcgaatta
    44521 tttcgctttg cgtggctggc aggcgaaaga tgtttttctg ccggatgatt atctgattaa
    44581 acagcgattt ccggggatga caccggcaca aatccgccgt tatgccgagc gctggaagcc
    44641 ctggcgttct tatgcgctgt tgcatatctg gtatacggaa ggctggcaac cagacggaac
    44701 ggacgaattg taggcctgat acgtcgcatc aggcaatggg caaacgctcc ctcagttaat
    44761 cgcgaaataa ctgctattca ccagcaaatc cagcggttgg gcatcggcaa tcacatcacc
    44821 ataaatcaga tcgacgccga tgccagaaag cgtatccatc actaagggta aaacgactgg
    44881 cccggcgatg gttttcatcc cgagtcgctg agcgtgcccc tgaataatcg taatcagcat
    44941 ctcatccatc aaattaccct gcacgttggc gcataactca ccatcaagta gcaggtaatc
    45001 tgccatattc gctttcagcg agttgaagat ttgcagatcg cggcccacct ggctgagcac
    45061 tatccgacat cccgccagcc gcagtttttg cacgctttct gcgtgatcta aaatcgcttc
    45121 agccggaata atcaggtgta ataaccgtgg tggtaaaggg ctattttcca gctgctcaag
    45181 cagatcattc accagcgtgg cgctactcaa accggcaacg gaaaggggga gggcgatgct
    45241 tatgccttta ctggcaaccg cttttgcggc cagctggaaa aattcgtgga atacccgtcg
    45301 gtcaagagca tggctaagtg ccggatcgct gaagctacga cgaaattttt gttcatcaat
    45361 aatctcgcct tcgcaactcc agagtttaag cgaaatcagc cacaaattac gcgcttccgg
    45421 gatccgcggc gaagcgacac cgtgggccat catcatcaac tgattctctt taatcatccg
    45481 ccactgttca tcaagcgaca tcgccgcccg ttcgctatgt gcggcagctt gctgtggttc
    45541 gtaaaccgtc acccggcccc gtccaccatt tttggaggca taacaggcga tatcagcctg
    45601 cgacatcact tctgccgcct gatgattgtt gtcatcaatc aaggtaatcc cggcactggc
    45661 acctacccga tgcacacggc cttcccatat aaagtgatag tcattcacgg cactgataat
    45721 gcgtgtagcg ataaaacgcg cgctttcaac attacagtct ggtagcagca gaccaaattc
    45781 atccccaccg agtcgcgcca gcacgtcgct ggaacgcagc atactcagca tcaacgacgc
    45841 cagttcgcgc agcaaagcgt cgcccgccgc atgcccggcg ctgtcattca ccgctttaaa
    45901 gcgatcaaga tcgataaaca ccagggcatg tcgctgatgt gtactgttta ccgtttgcag
    45961 cagcatacgc agttgcttct caaaactggc gcgattggcg agatgcgtca gtgcatcatg
    46021 ggaggcgctg tagctcagct ggcgcagcat tttgcgtgat tcggtaacgt cctgaatcac
    46081 cagaaccgaa ccaatattgc tgccgtccag agtacttaac ggcgtaatac tgtaatgcac
    46141 gtcgtagctg ccgccgctcc ggcagtgcaa caccacatct tgttcaatcg cggaacgtga
    46201 ggtgtcggca ctgtaaatgt tctccatcag gggaccattg tcgccaaagg taatatgcaa
    46261 caccgtcagg agcggaacac ctaatgcttc ttcctgcgtc cagccgctca tcttctccgc
    46321 cactggattc ataaaggtaa ttttcattgc catatcaata cagaccacgg cttcgccgat
    46381 ggagtccagc gttatgtgca ggcgctcttt ttcctgaaac aatgcctcgt taagctgttt
    46441 aacctcagtc atatccatat taatgccgag caggcgttcg acttcgcctt ctttattcag
    46501 tacccggttg gcgagggcgc ggatatggcg aataccgtct tttacggtaa tgcgaaattc
    46561 cagtttaaag ggcgagcgtg attgcaacga atcacgaatc actttttcgg catgctggcg
    46621 atcttccggc agcacgcact cgtaccacac ctgccagttc ggtttgatat gtggaggaat
    46681 ttcatacagc tcgaacatcc gcttatccca gctaaaaata ttcggcttca actcccactc
    46741 ccagatgcca atcccgcccg cttcgttagc cagcgtgatg cgctccatca gttgctgatt
    46801 cacctgttcg gtgcgtttta gctcgttaat gtcttcaatc tgagcgataa aatagagcgg
    46861 cgtgccatcc gtgtggcgca ccagtgagac ggcaagcaac gcccagacaa catcgccatt
    46921 gcggttgtag tagcgttttt ccattgaata ggtgtttatt tcaccgctta tcagcttttc
    46981 aacctgttgg agatctttat tgagatcctc cggccaggtc agttgctgaa aggtgagtcc
    47041 gcgcagctct tcctgactgt acccgagaaa ctggcagagc gctttgttgg tttgcagcca
    47101 ttgtccctcg gtgcccacta acgccatacc gatagcggaa tattccatgg cgttccgaaa
    47161 acgggtttcg ctttcggaaa tgtgtttgcg ttccgcacga aaggcataca tcaccatcgt
    47221 catgatgttg gcgggcagca ggatcagcaa aaacggtagc cacggcatat ggctcatcag
    47281 gtatgtacgc ggcgtagcaa gcagggaggg atccgcggcc atcatcagcg acaccatcat
    47341 caccgtggta aggaagatca aaaaggcttc cattcgtggc aggcgcacgg cgctccacat
    47401 caacagcaca ataatgaaag taaaaggcca cggcagatac agcatcgaaa gccagcttaa
    47461 cgtcagtgtg atggctaacg tgagcagcga ctcaaaaagc aaccgtgggt tgcgatggcg
    47521 cagcagatag tgtggtttaa ataacaatcc cagcggcacc agtgccagag cgccgatgga
    47581 ttctgacagt acccatatca aaaatgccct gagaggatcg tctccgggcg tcagcaggac
    47641 aaccagaaca ccccctagca gaggcggaac aatggcgctg ccgaatgcca gacgcagcca
    47701 gtcagccaga ttttgcaagg ggttatacca cggcagcaat ttacgtagca gcactgcccc
    47761 gaccacggct tcaacaatat tgatggtcgt ccaggtcatg ttcagcgagc tggtggaaaa
    47821 aagcaggatg gatgcggcga tatttcccag cgagcagctc agcgcaattc ccggccacat
    47881 gcgcccggca tggcgataaa acgccaccat catgatggac gtcgggaacc acagtggggc
    47941 gagttgggtg ccaaactgcg aaagctcaag cgagaaaagg gtaaagataa acgagactaa
    48001 acctaaactg accaggtgaa gcagcgggtg gggcagggca attaatacat gctgtgattg
    48061 tttgctcatt accgctttat ccgcaagacc aggtcgccag tattcgtttc tgtgtttatg
    48121 ctagtacaaa agttttacat tttatatgcg aattgctcat aaagtgacgt aaaggcggat
    48181 aatttgcgca actgcgtata acatttttta ccttgtataa aactgatcaa cgtaatttgc
    48241 caccgggaat tcttcgtcaa caattaactc tgagcgcgcg gcagcgctat tcgactggta
    48301 tcaggcggat gaaatcccta taattgccgc gtttggcgct tcgtcgcccc cttcctaaca
    48361 tccaggttaa tcaggtcgct aaatatatga ctgatcagtc tcatcagtgc gtcattatcg
    48421 gtatcgctgg cgcatcggct tccggcaaga gtcttattgc cagtaccctt tatcgtgaat
    48481 tacgtgagca agtcggtgat gaacacatcg gcgtaattcc cgaagactgc tattacaaag
    48541 atcaaagcca tctgtcgatg gaagaacgcg ttaagaccaa ctacgaccat cccagcgcga
    48601 tggatcacag cctgttgctt gagcatttac aagcgttgaa acgcggctcg gcaattgacc
    48661 tgccggttta cagctatgtt gaacatacgc gtatgaaaga aacggtgacg gttgagccga
    48721 agaaggtcat cattctcgaa ggcattttgt tgctgacgga tgcgcgtttg cgtgacgaac
    48781 ttaacttctc cattttcgtt gataccccgc tggatatctg cctgatgcgc cgcatcaagc
    48841 gtgacgttaa cgagcgtggg cgttcaatgg attccgtgat ggcgcaatat caaaaaaccg
    48901 tgcgcccgat gttcctgcaa ttcattgagc cttctaaaca atatgcggac attatcgtgc
    48961 cgcgcggcgg gaaaaaccgc atcgcgatcg atatattgaa agcgaaaata agtcagttct
    49021 ttgaataagc ttgataaatt gtgtaccgtt cagtgataac ctggtatgcc cttgacgtaa
    49081 ttggcgttaa gggagtgatg cgcgaaagga gaaaatgcca tgcgtctgtg tgaccgagat
    49141 attgaagcct ggcttgatga aggccgtttg tcgatcaacc cacgtccgcc agtggagcgt
    49201 attaacggcg cgacggtgga tgtacgcctg ggcaataaat ttcgtacctt ccgtggtcac
    49261 acggcagcgt ttatcgatct gagcggtccc aaagatgaag tgagcgccgc gcttgaccgc
    49321 gtgatgagcg atgagatcgt tctcgacgag ggcgaggcgt tctatcttca cccaggagag
    49381 ctggcgctgg cggtgacgct ggagtcggtg acgctgccag ccgatctggt gggctggctg
    49441 gacgggcgtt cctctttggc gcgtctgggg ctgatggtgc acgtcaccgc gcaccgtatc
    49501 gatccgggct ggtctggttg cattgtgctg gagttctaca actccggtaa gctgccgctg
    49561 gcgctgcgtc cgggcatgtt aattggtgcg ctgagctttg agccgctttc cggcccggcg
    49621 gcgcgacctt acaaccgccg tgaagatgcg aaatatcgca accagcaggg cgcggtagcc
    49681 agccgaatcg ataaagacta attcaggccc attgaggata ccatgagacg atttctgacg
    49741 acgctgatga tactcctggt cgtgctggtg gccgggttat ctgcgttagt gttgctggtg
    49801 aatccgaatg atttccgcga ctatatggtc aagcaagttg ctgcacgtag cggttatcaa
    49861 ttgcagctcg acgggccact gcgttggcac gtctggccgc agcttagtat cctctccggg
    49921 cgaatgtctc tcaccgccca gggcgcaagc cagccactgg ttcgcgccga caacatgcgt
    49981 ctggacgtgg cgcttttacc actactgagt catcaactga gcgttaagca ggtgatgcta
    50041 aaaggggcag tgatccaact gacgccgcag acggaagcgg tgcgcagtga agacgctccg
    50101 gttgcaccgc gcgacaatac cttgccggat ctgtcagacg atcgcggatg gtcgtttgat
    50161 atatccagtc ttaaggtggc ggacagcgtg ctggtgttcc agcatgaaga tgacgagcag
    50221 gtgacaatcc gcaatatccg cctgcaaatg gaacaagatc cccaacatcg tggctcattt
    50281 gagttctccg ggcgggttaa tcgcgatcag cgcgatctca cgatatccct taacggtacg
    50341 gtagatgctt ctgattatcc gcatgattta acggcggcta ttgaacaaat taactggcag
    50401 ttgcagggtg cagatttacc aaaacaaggt attcaggggc aggggagttt ccaggcccag
    50461 tggcaggagt cacataaacg cctttcattt aaccaaatca gcttgaccgc caatgacagc
    50521 acgctgagcg ggcaagcaca ggtcacgctg acagagaaac ctgaatggca gctgagactg
    50581 caattcccgc aactgaatct tgacaacctc atcccgctta atgaaacagc gaatggtgaa
    50641 aacggtgccg cgcagcaggg gcagagccaa tcaacattgc cgcgcccggt catttcttcg
    50701 cgtattgatg aaccggcata tcagggactg caaggcttta cggctgatat tttgttgcag
    50761 gccagtaacg tgcgctggcg cggaatgaat tttacagatg ttgccacgca aatgaccaac
    50821 aagtcgggtt tgctggaaat tactcaactg cagggcaagc ttaacggtgg acaggtttca
    50881 ctgccgggca cgctggacgc gacatcaata aatccgcgga taaacttcca gccacggctg
    50941 gaaaacgttg agattggtac cattctgaag gcgtttaact atccgatttc gttgaccgga
    51001 aaaatgtcac tggctggtga tttctccggt gctgacatag atgccgacgc attccgccac
    51061 aactggcaag gacaggcaca tgtcaaaatg accgacacgc gcatggaagg gatgaacttc
    51121 cagcagatga ttcagcaagc ggtagagcgt aatggcggtg atgtgaaggc cgctgaaaac
    51181 tttgataacg tgacgcggct tgaccgcttt accaccgatt tgacgttgaa ggatggcgtc
    51241 gtgacgttaa acgacatgca aggtcaatcg cctgtgctgg cgctgacagg ggaaggcatg
    51301 ttgaatctgg cagatcaaac ctgcgacacc cagtttgata ttcgggtcgt gggtggctgg
    51361 aacggggaaa gtaaactgat tgatttcctg aaagaaacgc cagtaccgct gcgggtttat
    51421 ggcaactggc agcaactcaa ttacagcctg caagtggatc agttactgcg caaacatcta
    51481 caggacgaag cgaaacgtcg tctgaatgac tgggccgagc ggaataaaga ttcccgcaat
    51541 ggcaaggatg tgaagaagtt gctggagaag atgtaggtta ccagatgacc tgtcctgtag
    51601 acctgataag acgcgtaaag agtcgcatca ggcgttcgtg gcggcatcat tatattctga
    51661 atatgtaaga tcgaaggctg ttccgttcac ttgatgttcc tcgctactat ggaacgctca
    51721 agccggtgaa cgtgccaggg tggctcaatc gctatcaccc tggcaacccg ggctcccggc
    51781 ggtaaatcgc cgctggcgcg gttccctcat cttattcctt tcggctatcg ggtaaaatga
    51841 ccgataccat cacacctcgt actccatctc gccatcctta cgcagcggta taatctgcac
    51901 cttctgcacg cgatggcttt ccacctgcaa cgttttaagc aaataatccc ccacctgaac
    51961 ttcttcgcca ggttttggaa tacgctgtaa atattccatc aacagcccgg caatggtgtg
    52021 atattcacgt ttttcatcca gcggcagcgg cacatattgc accagatcct ccagcggcat
    52081 atgaccattc gccgtccagg aaccgtccgc attcttctga atatcatggc gggcgtcgat
    52141 ctcttccact tcgttcggta agttaccggc aatggtttca gtgacgtcac ttaatgtcac
    52201 aatcccttcc accgagccaa actcatccac cacaaaagca aagtgcgtgc gggcattacg
    52261 gaactgctcc agggcaggta acaacggcaa ggtttccggg aacaccagcg gctggcgaat
    52321 caacacccgc aggttgagcg gttcgccgcg cagagactgt tgtagcaggt cgataacgtg
    52381 aacaacaccg agcaaatctt ctgcgtcatc accgtcggta accaccagcc gcgtatgctg
    52441 atttcgctcc agcaactggc ggatttctac ttccggcgcg ttgagatcga tatgctcaat
    52501 atcgtggcgc gacgtcataa tgctgctgac ggtacgctgg ttaagattaa gtacccgctc
    52561 aatcatccgc cgttcctgcg gattaaagat ctgctggttt ccatgatcca ccagcatgga
    52621 cgcggtttcg gcgtccagtt ccgcatcttc tttttgcccg ctaagcaggc gcattaccgc
    52681 ttcggtagtc cgctggcgta gcgtctgatt agcggaaaga aaacgtcgcc ggttaaagat
    52741 agccaactga ttgagtgcct cgatcatcac cgagaaacca atggcagcgt acaggtagcc
    52801 tttcggaatg acgaagccga aaccttctgc caccaggcta aagccaatca tcaacaggaa
    52861 gcttaagcag agaataacga tggtcgggtg gctattaacg aactgggtta acggcttgct
    52921 ggccatcaac atcaggctga tagcgataac cacggcggcc atcatgacca gtaaatggtc
    52981 gaccatcccg acggcggtaa tcactgagtc gagtgagaaa atggcgtcca gtaccacaat
    53041 ttgcgtcacc acgccccaga acttcgcacc tttgcgttgt gtgggattat tgctgtcttt
    53101 cccttccagc cgttcgttca gctccatcgt ggctttgaac aacaggaaga aaccaccaaa
    53161 cagcatgatt aagtcgcggg cgctaaaggt aaacgagcgg aagctgaaca gcggttgagt
    53221 cagggtgacc agccaggaga ttgacgccag cagtaacagg cgcattaaca tcgccagcag
    53281 cagcccggta acccgtgcgc ggtcgcgctg cttcggcggt agtttttcgg cgaggatggc
    53341 aataaagacc aggttatcaa tgccgaggac cagttcgatc acaatcagcg tgattaaccc
    53401 ggcccagata gacggatcgg caatccattc catagtaagt acaaaaccta ttcgttgtat
    53461 gacaaatgtc acaattcgat catggataaa tcggggcaaa aatgcaatgt tgcagcacta
    53521 tttatctcat attatttgag ggtaataacc tcacattatc cctg
//
LOCUS       DAZGSP010000022        52123 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZGSP010000022 DAZGSP010000000
VERSION     DAZGSP010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.022"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(146..1126)
                     /gene="ygfZ"
                     /locus_tag="ACHK74_001456"
     CDS             complement(146..1126)
                     /gene="ygfZ"
                     /locus_tag="ACHK74_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFP0220309.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            1369..1635
                     /gene="sdhE"
                     /locus_tag="ACHK74_001457"
     CDS             1369..1635
                     /gene="sdhE"
                     /locus_tag="ACHK74_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFP0220310.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            1616..2023
                     /gene="ygfX"
                     /locus_tag="ACHK74_001458"
     CDS             1616..2023
                     /gene="ygfX"
                     /locus_tag="ACHK74_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFP0220311.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(2063..2584)
                     /gene="fldB"
                     /locus_tag="ACHK74_001459"
     CDS             complement(2063..2584)
                     /gene="fldB"
                     /locus_tag="ACHK74_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFP0220312.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            2696..3592
                     /gene="xerD"
                     /locus_tag="ACHK74_001460"
     CDS             2696..3592
                     /gene="xerD"
                     /locus_tag="ACHK74_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFP0220313.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            3617..4327
                     /gene="dsbC"
                     /locus_tag="ACHK74_001461"
     CDS             3617..4327
                     /gene="dsbC"
                     /locus_tag="ACHK74_001461"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFP0220314.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            4333..6066
                     /gene="recJ"
                     /locus_tag="ACHK74_001462"
     CDS             4333..6066
                     /gene="recJ"
                     /locus_tag="ACHK74_001462"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFP0220315.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMILKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            6157..7255
                     /gene="prfB"
                     /locus_tag="ACHK74_001463"
     CDS             join(6157..6231,6233..7255)
                     /gene="prfB"
                     /locus_tag="ACHK74_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFP0220316.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            7265..8782
                     /gene="lysS"
                     /locus_tag="ACHK74_001464"
     CDS             7265..8782
                     /gene="lysS"
                     /locus_tag="ACHK74_001464"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP0220317.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(8825..9373)
                     /gene="idi"
                     /locus_tag="ACHK74_001465"
     CDS             complement(8825..9373)
                     /gene="idi"
                     /locus_tag="ACHK74_001465"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFP0220318.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSTFAQHN"
     gene            9428..9499
                     /locus_tag="ACHK74_001466"
     CDS             9428..9499
                     /locus_tag="ACHK74_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220319.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(9496..9621)
                     /gene="yqfG"
                     /locus_tag="ACHK74_001467"
     CDS             complement(9496..9621)
                     /gene="yqfG"
                     /locus_tag="ACHK74_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFP0220320.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(9623..11071)
                     /gene="uacT"
                     /locus_tag="ACHK74_001468"
     CDS             complement(9623..11071)
                     /gene="uacT"
                     /locus_tag="ACHK74_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFP0220321.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            11507..13426
                     /gene="ygfT"
                     /locus_tag="ACHK74_001469"
     CDS             11507..13426
                     /gene="ygfT"
                     /locus_tag="ACHK74_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFP0220322.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDCLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            13426..13914
                     /gene="ygfS"
                     /locus_tag="ACHK74_001470"
     CDS             13426..13914
                     /gene="ygfS"
                     /locus_tag="ACHK74_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP0220323.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(13950..15317)
                     /gene="ghxQ"
                     /locus_tag="ACHK74_001471"
                     /gene_synonym="ygfQ"
     CDS             complement(13950..15317)
                     /gene="ghxQ"
                     /locus_tag="ACHK74_001471"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFP0220324.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(15353..16669)
                     /gene="guaD"
                     /locus_tag="ACHK74_001472"
     CDS             complement(15353..16669)
                     /gene="guaD"
                     /locus_tag="ACHK74_001472"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFP0220325.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(16687..18087)
                     /gene="xanQ"
                     /locus_tag="ACHK74_001473"
     CDS             complement(16687..18087)
                     /gene="xanQ"
                     /locus_tag="ACHK74_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFP0220326.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEEVIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(18252..21122)
                     /gene="xdhD"
                     /locus_tag="ACHK74_001474"
     CDS             complement(18252..21122)
                     /gene="xdhD"
                     /locus_tag="ACHK74_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFP0220327.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(21119..21898)
                     /gene="ygfM"
                     /locus_tag="ACHK74_001475"
     CDS             complement(21119..21898)
                     /gene="ygfM"
                     /locus_tag="ACHK74_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFP0220328.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(21949..23277)
                     /gene="ssnA"
                     /locus_tag="ACHK74_001476"
     CDS             complement(21949..23277)
                     /gene="ssnA"
                     /locus_tag="ACHK74_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFP0220329.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVN
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(23280..26378)
                     /gene="ygfK"
                     /locus_tag="ACHK74_001477"
     CDS             complement(23280..26378)
                     /gene="ygfK"
                     /locus_tag="ACHK74_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFP0220330.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGYPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(26700..27278)
                     /gene="mocA"
                     /locus_tag="ACHK74_001478"
     CDS             complement(26700..27278)
                     /gene="mocA"
                     /locus_tag="ACHK74_001478"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFP0220331.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLIQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAKERYTEI"
     gene            27381..28151
                     /gene="yqeC"
                     /locus_tag="ACHK74_001479"
     CDS             27381..28151
                     /gene="yqeC"
                     /locus_tag="ACHK74_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFP0220332.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            28199..29824
                     /gene="yqeB"
                     /locus_tag="ACHK74_001480"
     CDS             28199..29824
                     /gene="yqeB"
                     /locus_tag="ACHK74_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFP0220333.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMADPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(29865..30797)
                     /gene="arcC"
                     /locus_tag="ACHK74_001481"
     CDS             complement(29865..30797)
                     /gene="arcC"
                     /locus_tag="ACHK74_001481"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP0220334.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGTSAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(30845..32230)
                     /gene="hyuA"
                     /locus_tag="ACHK74_001482"
     CDS             complement(30845..32230)
                     /gene="hyuA"
                     /locus_tag="ACHK74_001482"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFP0220335.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(32283..33494)
                     /gene="ygeY"
                     /locus_tag="ACHK74_001483"
     CDS             complement(32283..33494)
                     /gene="ygeY"
                     /locus_tag="ACHK74_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFP0220336.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(33552..34748)
                     /gene="dpaL"
                     /locus_tag="ACHK74_001484"
     CDS             complement(33552..34748)
                     /gene="dpaL"
                     /locus_tag="ACHK74_001484"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFP0220337.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(34806..35993)
                     /gene="ygeW"
                     /locus_tag="ACHK74_001485"
     CDS             complement(34806..35993)
                     /gene="ygeW"
                     /locus_tag="ACHK74_001485"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFP0220338.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            36472..38250
                     /gene="ygeV"
                     /locus_tag="ACHK74_001486"
     CDS             36472..38250
                     /gene="ygeV"
                     /locus_tag="ACHK74_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP0220339.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCCVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(38290..38769)
                     /gene="xdhC"
                     /locus_tag="ACHK74_001487"
     CDS             complement(38290..38769)
                     /gene="xdhC"
                     /locus_tag="ACHK74_001487"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFP0220340.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(38766..39644)
                     /gene="xdhB"
                     /locus_tag="ACHK74_001488"
     CDS             complement(38766..39644)
                     /gene="xdhB"
                     /locus_tag="ACHK74_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFP0220341.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(39655..41952)
                     /gene="xdhA"
                     /locus_tag="ACHK74_001489"
     CDS             complement(39655..41952)
                     /gene="xdhA"
                     /locus_tag="ACHK74_001489"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFP0220342.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            42367..43122
                     /gene="actS"
                     /locus_tag="ACHK74_001490"
     CDS             42367..43122
                     /gene="actS"
                     /locus_tag="ACHK74_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFP0220343.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            43201..43274
                     /locus_tag="ACHK74_001491"
     tRNA            43201..43274
                     /locus_tag="ACHK74_001491"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43233..43235,aa:Gly,seq:ccc)
     gene            43409..45007
                     /locus_tag="ACHK74_001492"
     CDS             43409..45007
                     /locus_tag="ACHK74_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005099221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0220344.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKK"
     gene            45050..45658
                     /locus_tag="ACHK74_001493"
     CDS             45050..45658
                     /locus_tag="ACHK74_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220345.1"
                     /translation="MVKEFLVSKENKPSYECMNTAETCLGLAGMASEDWQREHHCRTG
                     IIFTAFAIEAMFIFYRKQVDPGYDKTQKECRKTMHKNTLKLCGINNYMGTKPYQIIKE
                     CLEVRDAIAHGDSYTSSFNFSADHLDNQDDIAKSVLAVNSEQFRGLTVERLAKYIEMA
                     KHIDHEISDNGYRPSEGYLPAAERRSLMLAFGVSGVSMWPSA"
     gene            46491..46991
                     /locus_tag="ACHK74_001494"
     CDS             46491..46991
                     /locus_tag="ACHK74_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220346.1"
                     /translation="MSIKLTTMQAAYLTRAMTTGGGLIELGELAKGLGKQKAHVVRKL
                     EANFDKERLSKMETHLTVMGGNHKNVAQTVKTYMLDYKTAGALAMSYDLQLGVEVLTL
                     LEEAVQTIGAMTGAVQAGDTQAALEASLLFSERMRNRLEYTSGETENETRSVALKRLG
                     RKGASV"
     gene            46988..47173
                     /locus_tag="ACHK74_001495"
     CDS             46988..47173
                     /locus_tag="ACHK74_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220347.1"
                     /translation="MIDPRTPEGRLTLRYRGLRTSLLLSMLGLDKDATDNRPFYSRNE
                     LIERLVIRDMEFNRGNK"
     gene            complement(47154..47462)
                     /locus_tag="ACHK74_001496"
     CDS             complement(47154..47462)
                     /locus_tag="ACHK74_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220348.1"
                     /translation="MPKLAISLLLYLACFYAVGYGAGYITGGIAWHTLYMLERIITIA
                     LLAFIPVFIFAVLIKASKEQRIIKSLSVAAINIIPSAIALLVGFTDAVTRGFLTYFLG
                     "
     gene            47685..48176
                     /locus_tag="ACHK74_001497"
     CDS             47685..48176
                     /locus_tag="ACHK74_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220349.1"
                     /translation="MLFNNNDWKLSVTDINPYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQQNKRVEAERIAKEQRIQAD
                     RMREENMTDEEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            48260..49249
                     /locus_tag="ACHK74_001498"
     CDS             48260..49249
                     /locus_tag="ACHK74_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220350.1"
                     /translation="MNMTKNAFQFGIEPVHITDNDNLQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDTLYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            49923..51532
                     /locus_tag="ACHK74_001499"
                     /pseudo
     CDS             49923..51532
                     /locus_tag="ACHK74_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            51545..51664
                     /locus_tag="ACHK74_001500"
                     /pseudo
     CDS             51545..51664
                     /locus_tag="ACHK74_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            51624..52123
                     /locus_tag="ACHK74_001501"
                     /pseudo
     CDS             51624..52123
                     /locus_tag="ACHK74_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT        13146 a        12741 c        13395 g        12841 t
ORIGIN      
        1 tcggagcgca accgccggat gcggcgtgaa cgccttatcc gatctacggg cctcgtccct
       61 gttgtgataa aggcatcaaa tcccagacat gataagacgc gtcagcgtcg catcaggcga
      121 taaatgcctg atgcgataca gagatttact cttcgagcga atacggcagc ggctcgatat
      181 gcagtgtatt cgcatcgtcg cgtacgcgga agatgctgtc cggttccata tcgttattca
      241 tgacgacctg taccacgacc tgaccatctt ccagttttac cgcagccagc accgtaccgg
      301 tacgacgcca gttctcgccc attttcagct ctaagtcttc accagcttcc ggcagtcggc
      361 tggcgctacc tgccagcaac cagagcgcac gtttattggc accacggaat tttgctcgcg
      421 ccaccatctc ttgtccggta taacagcctt tcttaaagct gataccgccc agcgcctgga
      481 gattggtcgc ctgtgggata aactgcccgc tgttggcggc atcaatcacc gggaaacccg
      541 cttcaatgtt taatgccagc cactgttggc tattgttcag ttccgcttca ccgcgcagtt
      601 tatcggtcag catattagca gtagcttcat cggttacgat caggaaacgt tctgccgggt
      661 gttcaaacca tagcaaagtg gtcgcgcctt ctttgactac ctgtttttct ttcgaaggca
      721 gttcgctaaa gagatttgcc agcgcggcgc gcgcctgaaa accggcaaca ccaagcagca
      781 cacgctcgtc gtctggcgcg atggtcactt tagagaatac cgcatatttt ttcagttcag
      841 tcagctgcgg ttcacgcacg ctgcgccgtt caatccatgc aaagccatcg ccgtcgcgga
      901 acagacgtaa attgctccac attttacctt tggcgtcgca atgggcggcg agcaggtgct
      961 gatcttctgc catctggctg acatctgctg ttacctgacc ctgcatatat ttttcgctgt
     1021 ccgcgccagt aatggtggca agcgcccagt catcaagcgt catcagcgtc agcggtaaac
     1081 gggcagaagc cgtaggctga cggggaggaa aaggtgtaaa agccataata gagtcctgag
     1141 ttgcttaacg gcgtattaat gactaatggt aaaagagcta tgaggcaatg caagtgcttt
     1201 cactacgcga tctgtgcctt ctgcgaggcg acttccaaaa aaatgaagcc attgaattgt
     1261 tgtgaattaa tttcgcgatc gcgatggtgt ttactgatat tcccgtaaaa gaaggcgaaa
     1321 aacacgttac aatagcgggg tactggattt tcgtgagaca ggaagaagat ggacattaac
     1381 aacaaagccc gcattcattg ggcatgccgc cgtggtatgc gcgaactcga tatttcaatc
     1441 atgccgtttt tcgaacatga gtacgacagc ttaagcgatg acgaaaaacg catctttatt
     1501 cgtctgctgg aatgtgacga tccggacctg tttaactggc tgatgaatca cggtaaaccg
     1561 gccgatgcag aactggaaat gatggtccga ctcatccaga cacggaaccg ggaacgtggt
     1621 cctgtggcaa tctgatttgc gcgtctcctg gcgcgcacag tggctttcct tgctgattca
     1681 tgggctggtt gccgctgtta ttttactcat gccctggcca ctcagttaca ccccgttatg
     1741 gatggtgtta ctttcgctgg tggtgtttga ttgcgttcgc agccagcggc gtattaatgc
     1801 tcgccagggg gaaattcgct tgttgatgga cgggcgtttg cgttggcaag ggcaggagtg
     1861 gagcatcgtc aaagcaccgt ggatgattaa gagcggcatg atgctgcgtt tacgttctga
     1921 tggcggtaaa cggcaacatt tatggctggc agccgacagc atggacgaag ccgaatggcg
     1981 ggatttacgg cggattttgt tgcaacaaga gacgcaaaga taaagatgac ggcaaagcat
     2041 atgcgctaag ccgtcagtga gatcaggcgt aatgctctgc catttcgttg aggatttgct
     2101 cgcaccagct ctgaatacgc tcgtcgctaa ggtcatactg gttagtttca tccagcgcca
     2161 gacccacgaa cagttgcccg tcagcaatca ccggtttcgg gctggtaaat tcatatcctt
     2221 ccgttggcca gtagccgacg aacttcacgc ctttggtcga gagtttgtca tgcagcatac
     2281 cgagcgcatc gaggaaccac tcgccgtatc ccagttgatc gccaagccca tacagcgcaa
     2341 caattttacc ttcaaggttc aggtcgtcga gctgatccca gacggcttcc cagtcttcct
     2401 ggatttcacc aaaatcccag gtcgggatac ccagaatgag cacatcgtac tgctccatta
     2461 atttcgggga gtcgtccttg aggttatgta aggtcaccag ttctgggccg ataatatcgc
     2521 ggattttttc tgccgccatt tcggtgtaac aggtgctgga accgtaaaaa agacccatat
     2581 tcataacgta agcttctcga ttctgctgtg gcttttgcgg ctagtgtatc agaatcgctt
     2641 tagtcacggc acaataaggc ataatggatc aaatgagtga ccataagggg cgcaagtgaa
     2701 acaggatctg gcacgcatcg agcagtttct tgatgctctg tggctggaaa aaaatctggc
     2761 tgaaaatacg ttgaacgctt accgtcgcga tctgtcaatg atggtggagt ggttgcatca
     2821 ccgcgggttg acgctggcga cggcgcaaag tgacgatttg caggcattac tggcagaacg
     2881 gctggagggc gggtataaag ccaccagctc agcgcgtttg ctgagtgcag tgcgccgatt
     2941 gttccagtat ctttatcgcg aaaagtttcg tgaagacgat cccagtgcgc atctcgcttc
     3001 accgaaattg ccccagcgtt tgccaaaaga tttaagcgaa gcgcaggttg aacgtttatt
     3061 acaggcacca ttaattgatc agccactgga gctacgcgat aaagccatgc ttgaagtgtt
     3121 gtatgctacc gggctgcgtg tctctgaact ggtcggactg acaatgagtg atatcagcct
     3181 gcgtcagggc gtagtgcggg tcattggtaa aggcaacaaa gagcgcctgg taccgttagg
     3241 tgaagaggcg gtttactggc tggaaaccta tctggaacat gggcgtccgt ggctgctgaa
     3301 tggtgtgtca attgacgtgt tgtttcccag ccagcgtgcg cagcagatga cgcgacagac
     3361 cttctggcac cgcattaaac attatgctgt gctggcgggt atcgacagcg aaaagctgtc
     3421 accgcatgtg ttgcgtcacg cttttgccac ccatttatta aatcatggtg cggatttacg
     3481 cgtggtgcag atgctactgg gccacagcga cctctccacc acgcaaattt atacgcatgt
     3541 cgctaccgag cgtctgcggc aacttcatca acagcatcac ccgcgggcgt gatgtctgaa
     3601 aagaacggga agatttatga agaaaggttt tatgttgttt actttgttag cggcgttttc
     3661 aggttttgct caggctgatg acgcggcaat tcaacaaacg ttagccaaaa tgggcatcaa
     3721 aagcagcgat attcagcccg cgcccgtagc tggcatgaag acagttctga ctaacagcgg
     3781 cgtgttgtac atcaccgatg atggtaaaca tatcattcag gggccaatgt atgacgttag
     3841 cggcacggct ccggtcaatg tcaccaataa gatgctgtta aagcagttga atgcgcttga
     3901 aaaagagatg atcgtttata aagcgccgca ggaaaaacac gtcatcaccg tgtttactga
     3961 tattacctgt ggttactgcc acaaactgca tgagcaaatg gcagactata acgcgctggg
     4021 gatcaccgtg cgttatcttg ctttcccgcg ccaggggctg gacagcgatg cagagaaaga
     4081 aatgaaagct atctggtgtg cgaaagataa aaacaaagcg tttgatgatg tgatggcagg
     4141 taaaagcgtc gcaccagcca gctgcgacgt ggatattgcc gaccattacg cacttggcgt
     4201 ccagcttggc gttagcggta ctccggcagt tgtgctgagc aatggcacac ttgttccggg
     4261 ttaccagccg ccgaaagaga tgaaagaatt cctcgacgaa caccaaaaaa tgaccagcgg
     4321 taaataattc gcgtgaaaca acagatacaa cttcgtcgcc gtgaagtcga tgaaacggca
     4381 gacttgcccg ctgaattgcc tcccttgctg cgccgtttat acgccagccg gggcgtgcgc
     4441 agtgcgcaag aactggaacg cagtgttaaa ggtatgttgc cctggcagca actgagcggc
     4501 gtcgaaaagg ccgttgagat cctttacaac gcttttcgcg aaggaacgcg gattattgtg
     4561 gtcggcgatt ttgacgccga cggcgcgacc agcacggctc taagcgtgct ggcgatgcgc
     4621 tcgcttggtt gcagcaatat cgactatctg gtaccaaacc gtttcgaaga cggttacggc
     4681 ttaagcccgg aagtagtcga tcaggcccat gcccgtggcg cgcagttaat tgtcacggtg
     4741 gataacggta tttcctccca tgcgggcgtt gaacacgctc gctcgttggg cattccggtt
     4801 attgttaccg atcaccattt gccgggcgaa acattacccg cagcggaagc gatcattaac
     4861 cctaacttgc gcgactgtaa tttcccgtcg aaatcactgg caggcgtggg tgtggcgttt
     4921 tatctgatgc tggcgctgcg cacctttttg cgcgatcagg gctggtttga tgagcgcggc
     4981 atcgcaattc ctaacctggc agaactgctg gatctggtag cgctggggac agtagcggac
     5041 gtcgtgccgc tggacgctaa taatcgcatt ctgacctggc aggggatgag tcgcatccgt
     5101 gccggaaagt gccgtccggg gattaaagcg ctgctggaag tggcaaaccg tgatgcacaa
     5161 aaactcgccg ccagcgattt aggttttgcg ctggggccac gtctcaatgc tgccggacga
     5221 ctggacgata tgtccgtcgg tgtggcgctc ttgctgtgcg acaacatcgg cgaagcgcgc
     5281 gtgctggcaa atgaactcga tgcgctaaac cagacgcgaa aagagatcga acaaggaatg
     5341 caagttgaag ccctgaccct gtgcgagaaa ctggagcgaa gtcgcgacac gctacccggc
     5401 gggctggcaa tgtatcaccc cgaatggcat cagggcgttg tcggtattct ggcttcgcgc
     5461 atcaaagagc gttttcaccg tccggttatc gcctttgcgc cagcaggtga tggtacgctg
     5521 aaaggttcag gtcgctccat tcaggggctg catatgcgtg atgcactgga gcgattagac
     5581 acactctacc ctggcatgat actgaagttt ggcggtcatg cgatggcggc gggtttgtcg
     5641 ctggaagagg ataaattcga actctttcaa caacggtttg gcgaactggt tacagagtgg
     5701 ctggaccctt cgctattgca aggcgaagtg gtgtcagacg gtccgttaag cccggccgaa
     5761 atgaccatgg aagtggcgca gttgctgcgc gatgctggcc cgtgggggca gatgttcccg
     5821 gagccgctgt ttgacggtca tttccgtctg ctgcaacagc ggctggtggg cgaacgtcat
     5881 ttgaaggtga tggtcgaacc ggtcggcggc ggtccgctgc tggatggtat tgcttttaat
     5941 gtcgataccg ccctctggcc ggataacggc gtgcgcgaag tgcaactggc ttacaagctc
     6001 gatatcaacg agtttcgcgg caaccgcagc ctgcaaatta tcatcgacaa tatctggcca
     6061 atttagcgtc atcttctcta taaaaaagag cgtggattgg gtacaatccc gctcttatca
     6121 ccgcattttg actagctcaa taaaagaaat cagaccatgt ttgaaattaa tccggtaaat
     6181 aatcgcattc aggacctcac ggaacgctcc gacgttctta gggggtatct ttgactacga
     6241 cgccaagaaa gagcgtctgg aagaagtaaa cgccgagctg gaacagccgg atgtctggaa
     6301 cgaacccgaa cgcgcacagg cgctgggtaa agagcgttcc tccctcgaag ccgttgtcga
     6361 caccctcgac caaatgaaac aggggctgga agatgtttct ggtctgctgg aactggctgt
     6421 agaagctgac gacgaagaaa cctttaacga agccgttgct gaactcgacg ccctggaaga
     6481 aaaactggcg cagcttgagt tccgccgtat gttctctggc gaatatgaca gcgccgactg
     6541 ctacctcgat attcaggcgg ggtctggcgg tacggaagca caggactggg cgagcatgct
     6601 tgagcgtatg tatctgcgct gggcagaatc gcgtggtttc aaaactgaaa tcatcgaaga
     6661 gtcggaaggt gaagtggcgg gtattaaatc cgtgacgatc aaaatctccg gcgattacgc
     6721 ttacggttgg ctgcgtacag aaactggcgt tcaccgcctg gtgcgtaaaa gcccgtttga
     6781 ctccggcggt cgtcgccaca catcgttcag ctctgcgttt gtttacccgg aagttgatga
     6841 tgatattgat atcgaaatca acccggcgga tctgcgcatt gacgtttatc gcgcgtccgg
     6901 cgcgggcggt cagcacgtta accgtaccga atctgcggtg cgtattaccc acatcccaac
     6961 cgggatcgtg acccaatgcc agaacgaccg ttcccagcac aagaacaaag accaggccat
     7021 gaagcagatg aaagcgaagc tttatgaact ggagatgcag aagaaaaatg ccgagaaaca
     7081 ggcgatggaa gataacaaat ccgacatcgg ctggggcagc cagattcgtt cttatgtcct
     7141 tgatgactcc cgcattaaag atctgcgtac cggggtagaa acccgcaaca cgcaggccgt
     7201 gctggacggc agcctggatc aatttatcga agcaagtttg aaagcagggt tatgaggaac
     7261 caacatgtct gaacaacacg cacagggcgc tgacgcggta gtcgatctta acaatgaact
     7321 gaaaacgcgt cgtgagaagc tggcgaacct gcgcgagcag gggattgcct tcccgaacga
     7381 tttccgtcgc gatcatacct ctgaccaatt gcacgcagaa ttcgacggta aagagaacga
     7441 agaactggaa gcgctgaaca tcgaagtcgc cgttgctggc cgcatgatga cccgccgtat
     7501 tatgggtaaa gcgtctttcg ttaccctgca ggatgtgggt ggccgcattc agctgtacgt
     7561 tgcccgtgac gatctgccgg aaggcgttta caacgagcag ttcaaaaaat gggacctcgg
     7621 cgacatcctc ggcgcgaaag gtaaactgtt caaaaccaaa accggcgaac tgtctatcca
     7681 ctgtaccgag ctgcgtctgc tgaccaaagc actgcgtccg ctgccggata aattccacgg
     7741 cttgcaggat caggaagcgc gctatcgtca gcgttatctg gatctcatct ctaacgatga
     7801 atcccgtaac acctttaaag tacgctcgca gatcctctct ggtattcgcc agttcatggt
     7861 gaaccgcggc tttatggaag ttgaaacgcc gatgatgcag gtgatccctg gcggtgccgc
     7921 tgcgcgtccg tttatcaccc accataacgc gctggatctc gacatgtacc tgcgtatcgc
     7981 gccggaactg tacctcaagc gtctggtggt cggtggcttc gagcgtgtat tcgaaatcaa
     8041 ccgtaacttc cgtaacgaag gtatctccgt acgtcataac ccagagttca ccatgatgga
     8101 actctacatg gcttacgcag attacaaaga tctgatcgag ctgaccgaat cgctgttccg
     8161 tactctggca caggatattc tcggtaagac ggaagtgacc tacggcgacg tgacgctgga
     8221 cttcggtaaa ccgttcgaaa aactgaccat gcgtgaagcg atcaagaaat atcgcccgga
     8281 aaccgacatg gcggatctgg ataacttcga ctctgcgaaa gcgattgctg aatctatcgg
     8341 tatccacgtt gagaagagct ggggtctggg ccgtatcgtt accgagatct tcgaagaagt
     8401 ggcagaagca catctgattc agccgacctt cattactgaa tatccggcag aagtttctcc
     8461 gctggcgcgt cgtaacgaca ttaacccgga aatcacagac cgctttgagt tcttcatcgg
     8521 tggtcgtgaa atcggtaacg gctttagcga gctgaatgac gcggaagatc aggcgcaacg
     8581 cttcctggat caggttgccg cgaaagacgc aggtgatgac gaagcgatgt tctacgatga
     8641 agattacgtt accgcactgg aacatggctt accgccgaca gcaggtctgg ggattggcat
     8701 cgaccgtatg gtaatgctgt tcaccaacag ccataccatc cgcgacgtta ttctgttccc
     8761 ggcgatgcgt ccggtaaaat aagcattacg ttatgctcac aaccccggca aatgtcgggg
     8821 ttttttaatt gtgctgcgcg aaagtagaca acaattttct tgcttcacta ttggctgcct
     8881 gcatcaccat ccacggactg aacgcccacg gcgtggcatc aataccgtgt aatacatcag
     8941 ctaaatcaca ccattgataa tccatcactt catcatcgtt gatctgtaac gcactggtgg
     9001 tgcgtgcggc aaataccgga cacacttcat tttccacaat gccactcgga tcggtggcgc
     9061 ggtagcgaaa gtcaggatag atagattcag gaggcgtaat ttccacgcca agctcataac
     9121 ggcaacggcg gatcaccgcg tcttcgctgc tttctcccag ttgtgggtgc ccacaaaccg
     9181 agttagtcca cacgccaggc cacgcttttt tgctcagtgc gcggcgggta actaataatt
     9241 gccccttggc attaaacagc caactggaga acgcgagatg taagagggtg tctgccgtgt
     9301 gtgcggcata cttttccagc gtaccagtgg gaactccctg tgcattcaat aaaatgacgt
     9361 gttccgtttg cataatttct cacatgtaat tctgatcact catcattata gataattgaa
     9421 gagtgaaatg attaaccaag tgagcgttta tcgacaaccg cccgttttga gcggatgccg
     9481 acaggtaaaa accatttaaa aaagtagcat catatatttg aagcgacttt caatggccat
     9541 tgcaacacag tcagaaatga caggaataga gagagtaaaa agcaacagca gactgaatat
     9601 agcgcgcatt aaaagattca tattattctc catgctcatt tttcttcagc ttgcgcatca
     9661 gaagccacat acgtactgtc ctgactttta aatctttatc agagacggat tccttcacca
     9721 ggtcagcatg atgctgatag ccattaaaga agacgttcag cacaacggcg ctaagggttg
     9781 cgagcataat gccgctatgt agcagcggtt gcagtacggc cggtaactta gaaaagaaat
     9841 cgtgagagag cgtcggagtc atgccaacgc cgagactgat cgccacaata tagaggttgt
     9901 aacggttggt ggtgtagtta cagcgcgaca gaattcgaat ccctgtcgcc agtaccatgc
     9961 cgaacatcac cagcccagca ccgcccagca caaattgcgg aatggaggcg accagcaccg
    10021 ccatttttgg caccatgcca aacaggatta atataattcc cgaagaaata cacacccagc
    10081 ggctatgaac gcgcgtcacg ctaaccaggc caacgttttg tgaaaatgac gtgtggggga
    10141 agctgttaaa cgttccgcct atcattgtcc ctacgccatc gacacgcagc ccgcgaataa
    10201 tatcgtgtga agagagttta cgaccgacta tttcacccag tgccaggaac atccccatcg
    10261 actcgataaa cacgatgatt aacacggcag tcatggtcag aatggaaacg ggatcgaaaa
    10321 tcggcatacc aaatgacatc ggcgtgacaa tcgcaaacca tgaagcatca tgtagcccgg
    10381 ataaattgac ttcattcatc atccacgaaa gtaaaaagcc aaatacaatc cccagtaata
    10441 cggcgacgtt ggacataaat cctttcgcat agcgagtaat gagcaagata aaaattaaga
    10501 cagcaaagga gatacctaaa taaacgggat taccatattg cggattccct ttacctccgg
    10561 cggcccagtc aatacccacc tgaatgatgc taagcccgat agaagtaata accacaccgg
    10621 taaccagtgg cgggaataaa ggcatcaagc gaccgataag tggtgctaat aatgtggtga
    10681 taaaacccgc ggcgatagtg gcaccaaata tccccagcag gccgatatcc gggttcatcc
    10741 caatggctat cattggtgtt acagcagcaa aggtcaccga cataatcacc ggcaggcgga
    10801 tccccataaa gcggccgata ccgatacatt gcaataatgt gacgatcccg cagcaaaaga
    10861 gatccgagct aatgagcatc gcaatagctt cttttgagag gcccagtcgg tcaccaatca
    10921 taagaggaac agcgactgca cctgcgtaca tgacaaggac gtgttgcaga ccgagtatga
    10981 tcagctttcc tggtgataat atgcggtcaa cctcatcagt tgggcggtct tgcccagaag
    11041 atgagggaag ttgggaatct atggcgctca tcagtgtttc tcctttcgcc cgccagagta
    11101 aaaagcaacc gtttacgtta ataaaactgt ctaaacacat gctcgtggag tgccagtttt
    11161 tgcgaggaag gggagttaga gacaggaatt gcggaggcaa tcacataatc gtggttaaac
    11221 cacgaaaatg agaagagaac ctgacaatga aattatctgt aattttctca tattgatatt
    11281 tcattaatca ttctggcatg tcgcactctc gcatttaatc gtttttatct ggatagcgct
    11341 cttttggtcg gcaatgttga aataatgaaa gcggcataga ttgaggggca ataatttgaa
    11401 ctcagtcgca aaattataat taaaacggtc atgctaccta atatatgccc gtttattgca
    11461 tcgcgaaatt tgagaagaca taactcatta aatgaaaggg atgcaaatga ataagtttat
    11521 cgctgctgaa gctgcggaat gtataggctg tcatgcttgt gaaattgcct gtgcggtggc
    11581 acacaatcaa gaaaactggc cgctgagtca cagtgacttt cgaccgcgta tccacgttgt
    11641 agggaaaggc caggctgcga atccggtggc ctgccatcac tgcaacaatg ccccttgcgt
    11701 tacggcttgt ccggttaatg ctctgacttt ccagtccgat agcgtacaac tggacgagca
    11761 aaaatgtatt ggctgtaaaa gatgcgcaat cgcttgcccc tttggcgtcg ttgagatggt
    11821 cgatacgatt gcgcagaaat gcgacctttg taaccagcgc agttccggca cgcaagcctg
    11881 tattgaagtc tgcccaacac aggcgttacg actgatggac gataaagggt tacagtggat
    11941 aaaggtggct cgccagcgca aaacggcagc aggaaaagcg tcatcagacg ctcagccatc
    12001 tcgcagtgca gcgttgctcc ccgttaactc gcgtaaaggc gcagataaaa tttcagcgag
    12061 tgaacggaaa acccactttg gcgaaatata ttgcgggctg gatccactgc aagcgactta
    12121 tgagagtgac cgctgtgttt attgtgccga aaaagctaac tgcaactggc attgcccgct
    12181 gcataacgct attccggatt acatccgtct ggtacaggaa ggaaagatta ttgaagcggc
    12241 agaactttgc caccagacca gttccttacc agaaatttgc ggcagggtat gtccacagga
    12301 ctgtctttgt gaaggcgcat gtactttgaa agatcactcg ggcgcagtct ctatcggtaa
    12361 tctggaacgc tacatcaccg ataccgcgct ggcgatgggc tggcgtcccg atgtcagcaa
    12421 agttgttccc cgtagcgaaa aagtggcggt gattggcgct gggccagcag ggttagggtg
    12481 tgctgatatt ctggcgcgcg cgggagttca ggttgatgtc tttgatcgcc atccagaaat
    12541 tggcggtatg ctgacttttg gcattcctcc tttcaaactc gataaaacgg tattaagcca
    12601 gcggcgagag atattcaccg caatgggaat cgactttcat cttaactgtg aaattggccg
    12661 cgatattacc tttagcgatt taacttctga atatgatgca gttttcatcg gcgtggggac
    12721 ttacgggatg atgcgagcag atctgccgca tgaagatgcg cccggtgtca ttcaggctct
    12781 accgttcctg actgcccata cccgccagct catgggattg ccggagtctg aagagtatcc
    12841 gctgacggac gtggaaggta agcgagtcgt ggtattgggc ggtggcgata cgacaatgga
    12901 ttgtttgcgg acttccatcc gcctcaatgc cgccagcgtg acctgcgcgt atcgtcgtga
    12961 tgaagtcagt atgccgggct cgcgcaaaga ggtggttaat gcgcgcgagg aaggtgtcga
    13021 gtttcagttc aatgttcaac cgcaatatat cgcttgtgac gaagatgggc gtttaactgc
    13081 ggtgggcctg attcgtaccg ccatgggtga gccggggccg gatggtcgcc gtcgtcctcg
    13141 tccggtagcg ggttcagagt ttgaattgcc cgccgatgtt ctgattatgg cctttggttt
    13201 ccaggcgcat gccatgccgt ggttgcaggg cagcggaatt aaactcgata aatggggcct
    13261 gattcaaacc ggtgacgtcg ggtatttacc tacccagacg catctgaaaa aagtctttgc
    13321 tggtggtgat gcagttcatg gcgcggatct ggttgtcact gcaatggccg caggaaggca
    13381 ggcggcgcgc gatatgttaa ctctgtttga tacgaaggca tcgtgatgaa atcgttaatt
    13441 atcgttaatc cggctgactg tattggctgc cgtacttgtg aagtagcctg tgtggtcgct
    13501 cacccttcag aacaggagtt gaatgccgat gtctttttac cccggctgaa ggtacagcga
    13561 ctggacagca tcagtgcgcc ggtgatgtgc catcagtgtg aaaacgcccc ttgtgttggc
    13621 gcttgccccg tgggggcgct gacgatgggc gagcaggtgg tgcagaccaa ttctgcccgt
    13681 tgtattggct gtcagagttg cgtcagtgcg tgcccgtttg gaatgatcac cattcagtcg
    13741 ttgccggggg atacccgaca acaaattgtg aaatgcgatc tctgtgaaca gcgggaagag
    13801 gggccagcct gtgttgaatc ttgcccgaca caggcgctgc agttgcttac tgaaagagaa
    13861 ctcaggcgag tccgccagca gcgtattgtt gccagcggtg agaatcctct gtgaaagaaa
    13921 tctccgcgct gaaaggcgcg gagaatctat tagattgccc aaccacccgc gtaaaatgcg
    13981 accagtgcgg cagtaatgat caccgtacca atattcaact tttgccattc gcgagcaaag
    14041 acacggccta cgaccagtgt cacaaagccc agcataatac cggtaacgat attacaagtc
    14101 agaacgatga acacggcaca caccaggcca gccattgcgt caataaaatc gttgaaatcc
    14161 agcttcgaga cgttacttaa catcagcaaa cccacgtaca tcagagcggg tgcagtggcg
    14221 taaccaggga tcaaaaatga cagcggtgat aagaacagaa tcagcaggaa taacgccccc
    14281 actacggttg cggtcaaccc tgttttaccc ccggcggcgg ttcctgccgc tgattcgata
    14341 taaaccgctg cgggcgctgc gcccaccaga ccggagaata ttgaacttac tgagtcactg
    14401 gtcagggctt tgccgccatt gatgatctgg ttgtctttat ccaacaaatt cgcctgaccg
    14461 gcgacggcac ggatggtgcc agtggcgtcg aacactgcgg tcatcaccaa tgccagtaca
    14521 ctcggaagta cggttggctg gagtgcgccc ataatatcga ggctgaaaat cagagactta
    14581 ccatcttcgc cagtcagact tggcatcgcc accagaccgt ggtatttcac cgctgggtca
    14641 aagattaagc cgataatcga aattgcgata atcaccaaca agatcccgcc gggtacgcgg
    14701 cacttctcca gaccgaagat gaccgccagc cccagcaagc tcatcatcac cgggaaggag
    14761 gtaaacgcac cgagcgccac tggcaagcct tcaatcgggt ttttgataac catacccaca
    14821 ccgttagcag caatcagcag cagaaacagg ccgataccga tacctgtacc gtgagcgata
    14881 cccatcggta aattacgtaa gatccaggta cgcacaccgg ttacggaaat agcggtgaag
    14941 atgacgccca tcagaaagac cgcgcccagt gcgacaggaa cgctaatttg ttgcccgagt
    15001 accagactga atgcggtaaa tgccgtcaag gaaatcgcac aaccaatcgc cattggcaaa
    15061 ttggcccaca accccatcag caacgagccg aagcccgcga ccagacaggt ggcaacaaac
    15121 acagctgcgg gaggaaaacc tgctttgccc agcattcccg gaacgacgat aacggaataa
    15181 accatggcca gaaaggtcgt taagccagcc agtacttcct gacgaacggt actgccacga
    15241 gcggtaattt taaaataatt atccagcgcg ccctgtggct ttggtgcgtc cggtgtttgt
    15301 aggatgtctc cagacataat gatgtcctca cgaggatgtt aaagttttat tattagttgc
    15361 gttcgtacac cagacgacca tcaacgtagg tgcggtagat cgaacggtca tcgcccaacg
    15421 tcatcatcac gaacaatttg tcgactaaag aaacagagtt gtcatagcgc agctgctgta
    15481 gcggagtggc ggtgggttcc atcaccacga aatcagcctc tttgccaggt aaaaagttgc
    15541 caatcaaatc gtcaaggccc agagatttcg ctccgccgag cgtggccagg taaaacgctt
    15601 catatgccga gaggcgatag ccttgtaatt gcaatacttt gtaggcttcg ttcagcgttt
    15661 gcagcatgtt gaaagtggtt ccggcaccga tatccgttcc catgcccact ttaactttct
    15721 tctgccatgc ttttttcaag ttgaataagc cgctgccgag gtaaaggttg gaggtcggac
    15781 agaaagcaat gctggatttg gtttcgctga gacgatccca ctctttttct tcgagatgga
    15841 cgcagtgagc aaagacacag tttttaccgg tcaggccgta ctgatggtaa acatccagat
    15901 aaccatcatg gtcaggataa agcgatttca cccaggcaat ttcatctttg ttttcacaga
    15961 gatgggtatg tacccacgta tccggatatt cttctttcag gcgttgcgcc atcgccatct
    16021 gttcaggaga tgaggtcggg gcgaagcgtg gcgtaatcgc atatagcaga cgaccatttt
    16081 tgtgccagcg ttcaatcagt tctttgcttt ggtgatagct gctttcggca gtgtcgagca
    16141 gataatccgg tgcgttgcgg tccatcatca ccttaccggc aatcatacgc atattgatat
    16201 gactggcggc ttcaaacagc gcatcaacag attgcggatg aacagtgcca aacaccagcg
    16261 ccgtggtggt tccgttacgt aaaagctgct tgatgaagaa tgccgacatt tcgcgggcgt
    16321 actctaaatc ctcataacga cgttcagtag ggaaggtgtg tttattcaac cactccagca
    16381 attgttcacc ataggccccc accatttcac tttgcggata atggatatgt gtatcgacaa
    16441 agcccggtac tatcagtttg ccgcgatagt cgcgcacgcg aatagtgtca ggaatttgat
    16501 gctttccgtc ttcccattcg ccaaaccatt ccacttttcc ctgtttaatg agtaataaac
    16561 catcctcaat aaaccgcagc gcagaggcaa tctcttccgg gttatcgacc gtacgggtga
    16621 catcaataaa actgcctcgt accgctttca acgtgtgttc tcctgacatc attgactcct
    16681 ttactgttaa tccatctctt ccgctgaggt aataccaggc agaacgtttt cctgtcggta
    16741 gccaccaggg aggataatat ttaataaaat cgcagttaac ccgccagcac aaatagggtt
    16801 ttcaactagt acataaatag aggctggcaa tattttaaaa atttcgggat cgtaggagac
    16861 gccaagtcct aaacctaaag aagtggcgac aataagtgtt tctcggcgct ttaaaccgtt
    16921 ggtgatgatt atgcgaatcc cagcgatggc aatcatggaa aacatcaacg tcattgcgcc
    16981 tcccagaact gccgagggaa tggtcgtgaa gaagccgcca atcatcggaa acaaaccgag
    17041 gataaccagc attaccgcga tggttcgccc gacataacgt gaagcgacgc cagtcatctg
    17101 aataacccca ttattttgcg caaacgtggt taagggtaat gacccgacag cggaggcgat
    17161 aacagaaacc agaccatctg ccagcacgcc gcctttcagt cgggactgat actcttcccc
    17221 ctgaatgggg cggcgggaaa ccattgccgt ggcggtgata tcgccgactg cttccagcac
    17281 gctaagcaga taaatcgtgc caaccaccag gaactgatgg aagctaaaac taaagccgta
    17341 tttgaacgga tgcgggatgg tgattaacgg caaattgcgc atactgctga aatccaccat
    17401 gcccaggcat aacgatgcga tatagccgac acatagccca atggcgatcc ctcccatgcg
    17461 tagcaacgga ctgcgacagc agttaaagcc gatcaccaca attaaaacca ataaaccaac
    17521 gccgagatgt tcgtaattgc cgaacgtacc gctgcttttg gctgcaaatc ctccaccaaa
    17581 atcgataatg ccgactttaa tcaggcttaa gccgatcatc agtaccacaa taccgctgac
    17641 ggtaggcgta ataacccgac gtaaataggg caagataaat gaagatccga caaccagaaa
    17701 tgcgccaacg aaggagacgc cgagaagcga cgacatgatt acctcttcgt gaaaaccgtc
    17761 gcttttcatg ctgctgccca gcgcaatcat gaccgtaaca aatgaaaaat tgactgactg
    17821 aattgagagt aggccagaac cgacgatgcc gtagcggttt acttgtaacc aggtaccaat
    17881 accagaggcg atcatcgcca tagaaacaag ataggcggtt gtttcagcgg aaagctgtaa
    17941 ggccgcaccc acgattaacg cgggggttac catcggaacg aaaattgcca acagatgggt
    18001 aatggcacca acgagagcct gatgaaaggg agggcgatcc tccagttcaa atataaggtc
    18061 agaacctgca tggtttatat cagacatccc ttcactcctt ttaaagtaaa tttgatcatc
    18121 tgaattagta aaataaattc ccacgaattt ggggcgcagg acgccttgag aacggcttat
    18181 ctcattttcg cagtcactat ttagtcatcg tccaaacaga gaaacaggcc gccgaagcgg
    18241 ccatttatta tttatatttt ttccagcgca gtgagtattt tctccggtgt gaaatgccat
    18301 tcgcgtaacc agatgccgca tgcatcgtga attgcggtag caatcgccgg agctgcgcca
    18361 tttacaccga tttccgagat cgatttcgcc ccgaacgggc cgactttatc gtcgctcggc
    18421 accagcacag cgcggaaatc acgcggaatg tcaccaattt tcggtgcgcc gtaactgcgt
    18481 aaatcacgcg ttaacgggtg accttcggcg tcataaatga tctcttcgct catactgtgg
    18541 ccgatagctc gcagggtagc accgtagatt tgtcccaatg ctaattccgg gttgaccggt
    18601 gtaccgcagt ccagcaaggc gtagaattta tccaggcgga tttcacccgt acgcgtgttg
    18661 acggcaactt cagcgaagtt tgcgccatac gggaaggcga aatcaggcgt gatataactg
    18721 ccagttccca ccagtgaacc aaagccggtg ccggtttcgc ctttatgagc aatatccccg
    18781 aagctgactt cgcctttctt gccgcgcacg acgcccggcg ttgccagttg aacatctgcc
    18841 actgcctcac ccaacatttg cgcgccgtgg aacagaattt tctcccgcag attttccgct
    18901 gccaaacgcg ccgcgttacc tgagaagcaa gtaccggacg aggcatatgc gcctttatca
    18961 aacaacgcat gatcggtatc accggagata acatgcacgt cctgcggtgg gcagtgcagc
    19021 acttctgctg ccagtttcgt cactacagta tccagaccag taccaatatc cgcaccgcca
    19081 gaatgaacga taaaggtgcc gtccgattcc agtttgatca tgcagttagc ctgatcgata
    19141 tccgggatac ccgatttctg catgataatg gcgacgccgc gaccgatgtg ccagtcacca
    19201 ttttgcggtt tcggtgacga ccattggatc atctcgcgac cctgacgcag gatctcttct
    19261 agtgcacagc tggcggcgga aggaacggag gtcggcgctt taccttcacc gattgcaccg
    19321 agaattttca gctcttgccc ttcgtgtacc cggttacgtt cgataatttc cagttggtcg
    19381 atctgtaact gttcagccag ttccgctaat gccatggtga tagcgaagtt acctttcggt
    19441 gcgccataac cctgataagc accatttggg caaatgttgc tgtagtaggt ggtgacctgg
    19501 aaatcgacgt tatcgcacgg atataacggc agcgacagcg ccggtccgtt acacggtacg
    19561 gtgagtgagt ggttgccgta agggccagtg ttggcgcgga aatccatctt cactgccgtc
    19621 aggcgaccat cttttttcgc tcccagtttg acggtgactt tcgcgacgtg acgagaggtg
    19681 ttagcaataa actcttcttc acgggtgtag cggaacagta ccggacgccc ggtcacgcag
    19741 gttgcccagg cgcacacttc ttccagcagg atgtcctgtt tggaaccaaa accgccgcca
    19801 actcgctctt taatgacatg aactttatgc tgtttcatgc ccacgaggcg cgcgacctgg
    19861 cggcgtaagt gccatggtac ctgggtggag gcgtggataa ccagacgatc gccgtccatc
    19921 cgggtaaagc agatatgtgt ttcagtcggg cactgctgtg cctgcgttga gttataggtt
    19981 cgctcaatga tcacatcggc atcggcaaag cctttgtcca tatcgccaat atgaccatga
    20041 atactggcgg cgatattttt gcgagggcga gaaccgatcg ggaagttgat gatcatatgc
    20101 tcgccgcgct gggctgcatg gctgttatcg tcttccagag tatctggcgc accagcaaca
    20161 tacaccaccg gttcatcgtg cacgacaggc gcatcttccg ccattgcttc gtcgatcgac
    20221 attaccggct taagcacttc atattcaacg tcgatgagct tcaatgcttc gagcgcaatt
    20281 tcttcacttt ctgcgacgac cgcagcaaca cgatcgccga cgtgacgcat tttcttgccg
    20341 aacatacggc ggtcaagcgg tgacggttcc ggtgcgctct gaccacccgg ggtatagtag
    20401 atatccgggc aattcaggtg agtaataacg tgaacgacgc ccggtaaggc ttcagctttg
    20461 ctgacatcca gatgagtaat cagtgcgtga gcgtgtgggc tacgtaacat tttaatgacg
    20521 caggcgtcag ccgttacgcg gtcttcaaca tagcagggtt tcgcctgcac cattttcgcg
    20581 gcatcagttt taggataatg cttgccaatg acttcaaggt cgtcacggaa agtcggagcg
    20641 atatcaatgg tggcctgcgg attattttta cgtgcaaccg ccagttcaat gacctgatag
    20701 tattgttgcc agcctgcatc gcggctgaac aaaccagaaa gcgcgtcgtc gatctcttcg
    20761 cgggtaggtg cggcgatgcg atcgagaaga tcggtgataa tcagagctgc agctggatcg
    20821 ttataaccag actgcaccac gccaacatca accattgcct gttgaaccag acttaactcg
    20881 ttccatttgc ccaaagattc tgcggtacga atatctgcct tctctaactg tgcggcaata
    20941 agcaaggacg cgttaacgat attaccgtta aagattattg cgtcagaacc ggcaaacccg
    21001 aagccatcat cactgttgcg tacagagtgc attcccatgt taaacaacag cttttgcacg
    21061 ttttcgcctg gattaacggt tagctcctga ggcgcgccat ttaaagtaaa gtggatgatc
    21121 atacggcttc ctccccagcc tgttggcagt cggcatacag gtcggctacc agaactcccg
    21181 tgatatagcg tttataggcc acgctgcccc gcaaatcttc ctgcgggaaa atggcgttgg
    21241 cgacagcctg ttcaagtgca ttgccttcca gattttgttt ttcgacatca tgcagacgca
    21301 gtgctttact ggcgacgcca tccagcgcaa ttcgcatacc gtcgtggtct gtcattgcaa
    21361 cggctgccgt cacgacggtt aaacctgcct gagaacggct aattttgcgg gtcgcacagg
    21421 tgcgatacgg atctttaatg ataatttcgg ttagcaggcg atcgcatggg caggccaggt
    21481 agtcctcgat tgacagcgtt tcgccgttgc caaaaaccag ttcagcatcc agtgccaaca
    21541 ggacgggaag cagcaccgac tcttcctggc gggcggcgat ttcaccacca atggtcgact
    21601 gattacgaac atggcgtgag taaacaaaac cgagggcttc acgcagcgct gcaggaataa
    21661 atcgcgcatc acgcagtggc tgcaagcgag acattgcgcc aatccgcagt gcaccgttat
    21721 cccagtcaac ccagtccagt tccagatcct gtaaggaaat ggcaatcttt ttatcggtac
    21781 gggttggtgt agcattgagt ttgctgcccc cggcgaacca gacggcttca tcctggtagc
    21841 ggcgcttcag ttccagcgcc tgttcgacag agtcgggcct gaaaaattgt tcaatcatct
    21901 tctttcctta tttaaccctc ttccctgcgg gaagagggca tctgtcattt atgccagcgc
    21961 atccatccga cgccacatac tggcagcggc ttttctggct tgtgcataaa tggaatcgca
    22021 atcgaagtta aactgacggt cttcatagac catcacacca ttgaccatca cgctgtgaac
    22081 gctgcctgag cccataccga aagcgatatg cccggcgata ttgtctgcca gcagcggcgt
    22141 cggtgagttg taatcgcaaa tggttaaatc agctttgtaa ccggcttcca gaagtccaaa
    22201 tttcgcgcca aagttgcggc tcatcagttc gttaccgtta gtcagggctt tggcaaaact
    22261 gtcaggccac agcggaccac ccgcatcgcg atgtttaaag aaggcaaatt tcatctcttc
    22321 aaacatgtcc gaaccaatgc cgtccgttcc caacgccaga ttgcggatgt cgctaaggtg
    22381 atggttgtag ccgacatggt tgttcatgtt tgaacgggcg ttatgcacca ggaacgcatc
    22441 gcgctgattg agtagggcga tgtcatcttt cgacaagtac agcccatgag cgaccagcgt
    22501 tttgctatcg atgagatcga attgcgctag tcgtgccagc aggtctttgc cgtaccagtg
    22561 gtgactgtag gaaacgtcgt aaaggtcttc cgcagcgtga atatgcaaac cacggcctgt
    22621 ggcgttcacg gcttcacgta gcatctccag accggcatcc ggcacggtaa acggcgcgtg
    22681 agcaccgata tgtgcttcca ccagatacgg ctcggttgct gctttcttcg cctcatcaat
    22741 ctgacgggcg aagcggatgt tttcttctac accttcctgc aactctttga tgccgttgtt
    22801 acggtcagta gtttcaaaac aggtcattgc gcgcaggcca acttttaaaa atgcgtcgcg
    22861 caatgtggag agcgacccgc cgatatacgc cggagaggca tggtgatcga taaccgatgt
    22921 acatccgctc ttaatcgctt ccagggaaca aatcagcccg ctgtaataga gcgactcttc
    22981 atcaagggcg cgatcgagcc gccaccagag atttttcagc gttgagatga aatccgggca
    23041 gggggcgatg tttgccataa ttccgcggga aagccccgag taaaaatggt tgtgcgagca
    23101 gacaattccc ggcatcacaa tccgaccatg catctctttg tagctggcat cggggtagcg
    23161 ttgcgtcagg gcatcgccga tagcgacaat cacatcattt tcgatagcga tatcaacgcc
    23221 ttcctgcact ttcgccgggt gtaactgcac tgcggtgaca ttcttcagaa tcaacatgat
    23281 tacacctcca cgcggcccag cagataatga tgatgctggt ggacatggct gatgatgcgg
    23341 cacatatcgt tcagctccgg tggtacgttg ttgaactgac cttcgctgtc gatgtttaac
    23401 acccagcttt ggttattcag tcgtactcgt acccggcaat cttccacaag gaagcctggg
    23461 ttgctgctgt tatcaaagtc ttgcgccagg ctgaaaacgg tgattttgtc tttgtatggt
    23521 ttaccgttcc acggacagaa ctgagcgcag ttgccgcatt cgttacagta agcgtcgagg
    23581 tgcagcgtct ggaatctatt ctggaagcct gggaccgcaa tggagacgtt ggcgcggttc
    23641 gggcagacat ccacacactt gctgcaaacg tagttacatt cgaggcagcg agcggcttcc
    23701 tgggcgacaa acgcgtcacg atcgtcactg ttcaccagag tgatagagat atcgccttta
    23761 cgttgataga tttccgccgg attgacgttg ttccagtatt tatcgttctg gtgggaacgg
    23821 atattttccc gactaaggat ggcatcggtc gcccgacgcg cggttccgac agcagcgaca
    23881 atggaggatg gtccgcgctg tacgtcgccg atcataaaga cgtcagtcag acgagtttcg
    23941 ccattgtggt cgacgtctgg ccagccgttt ttgtccagcg gcacgcccat cgcattcagg
    24001 gcttcagtat cctgctgttc accaatggcg gtgatcaggc tgtctacatg cagtgttact
    24061 gtttcgttgg tttcaaccgg acggcgacgg cctttctcat cgggttcgcc aagcgacata
    24121 acgcgcaagg ttaaggtgcc atcagcatcg aaacgttccg gattattcag gaaacggaac
    24181 tccacgccgt cgtgcaacgc ttcttcatac tcttcgcgcc atgcgggcat ctcttgcagt
    24241 gaacgacggt aaacgatcgt tgctttttct acgcctggaa cgcgtaacgc cgcacgagcg
    24301 cagtccattg cggtgttacc cgccccgaca acgaccacat gtttgcccag cttgagcgct
    24361 gtacccttgt tgtattcacg caggaagggg agtgatttcc aaacattttg gttgtcaccc
    24421 gccagtttca caccgctatt tttatcagtg ccggtggcaa tcagaacata gtggaagccc
    24481 tgatttttta actgttcaac ggtcaaatcg ggtgagcagc catactcaaa tttcacgccg
    24541 tgatcggcaa caaaatcgat atcgtgctga attaactcag ccggaattcg gaactgagga
    24601 atgatatttt tcaccacgcc gcccgcattg gcttcgcgtt caaacagcgt aaccggatag
    24661 cccgctctgg caaggaagta acctgctgcc agacccgccg gacctgcacc aatcacggca
    24721 accggatggc gtgaaccaga accggctggt ttgtgccagc gttgcttata ttcatcccaa
    24781 cctttttcca gcgcgacttt tttcagttcg cggatattca gcgcactgtc gtaatccagg
    24841 cgggtacagt tgtattggca ctggtgatcg caaatatgac cggtaatggc gggcagagcg
    24901 ttgcgttggt agatgagttc cagcgcgtcg gcatagcggt gttcgccaag cagacggatg
    24961 tattccggaa tatcttgctt gatagcgcag gcagtaacac agggggcaac gtagcagtcg
    25021 gtcagcggca ggtcttctgc cacttcaata cgctcttctg gcttccagtg tttctgggtg
    25081 tattccatgg ttaacgcatc tgctgccagt ctgttcagtc gttcgacgtc aacatggtca
    25141 agcccccagg cgtcggagcc ttccagctcg cgcatgcagg cacttaagcg cagatagccg
    25201 ccaggtttca gcaggtcggt tgccatagta atagggcgaa taccagtatc aaaaatatcg
    25261 cggatagtca gctgactggc accaccggaa taagaaatgg gcagtttgcc gtcaaaggcg
    25321 cgagagagaa ctgctgcaac attgatggag agcgggaaca gcgcacggcc tgacatatac
    25381 atctcttcac caggcagtgc gcctttattg ttgatggtgc cgagagtgtt agtcagtttt
    25441 acgccaaagc cgagtgattt ttcttttgcc agtgccatca ggcgttccag catttccagt
    25501 gcttgcgtca gcttgaggtc gtgatcaaat gactcttctt ttaagcctat gtaaccgaaa
    25561 ccgcagacat cgaggatctc acgaacacgc gcgtacccca gtaaggtcgg gttgagtttc
    25621 acaaaggtgt tgagcccttt ttcttccagc atgtagcggc aaatggcttc gatttcatgc
    25681 ggaggacagc cgtgcatggt ggagagggtg acgccctgca ccatactggt ggggatgcga
    25741 gcgggtaagg cttgcaagct ttcgcgtttt tcctgcaatc cgtggcgggc taaaaatgcg
    25801 tcatcctgga gtaatttatt cagcgtatcg cgatattgag cgaatttcgg atggtcagat
    25861 gcgtccatca tattgtcgat gaactgttgc atcggcggtt gcttaatacc ttcgaggttg
    25921 taaccgacgc tcatattaaa gatgaacgat ttaccggaat cagaaggctg gaacatcgct
    25981 tcgagaaggt gcagggcaaa ccaggctttg aggtattcat cccaggcttt aagcagggta
    26041 aattcggtag accattcggt gttaaaacac tcgtcttcgg catcgataca gggcttttcc
    26101 agctccaggc ggtcaagaat ttggacggtt tttagttcga tgaagcgtcc gccagtcagc
    26161 caggaagtga caatattttg cgcgagctgc gtgtgcgggc cagcggcagg gccgacggga
    26221 gtggcacagg tttcaccgaa gacgctaaca gttttacctt ttacgggtga gtaaaactgt
    26281 tgctcgggaa taccaaagat tgagcgttgt tgttggtatt catcaaatat gcgcgtcaaa
    26341 agttcctcaa acggaatggg acgcataata tcccccataa ccctctcctt agcttataca
    26401 gatatcattg agtgaccggt gttgagaaat ccggtggttt agggggatag agcaacaatc
    26461 gcaccactct tgccaggttt ggcgggaaat ataaaaaaat gtgaggcggc tcgaaactta
    26521 agggcttttt gtgaatgaac tcacaggtgc caaatgagaa ttaaaatcac tcctgaaaat
    26581 tattattgcc agcccattta tcaatttttc tcttttctca aaatcagtga ttccttatca
    26641 ttatgataat gagtgccgga aggattaaat atttgaacgc aatcgtttaa ctacgttttc
    26701 taaatttcag tatacctttc cttcgctgta ataaagtcgt ccggagtatc aatatctaaa
    26761 attatttctg cattttctat ttcgacggaa taatgatctc ccataaggag tgcctgacgc
    26821 atattggtga cattgggtcg ttggattgcc tgtatcaagc atggttttga cactaaaatc
    26881 ggatggccgg ggatgccatt atggagtggc agtattgcgc catcatttcg taacgaccag
    26941 atttttctaa aaatatcgat ggtgagggtt ggcatgtcac cgtgggtgag aaaacaatgt
    27001 tctgtttgca ctgcgggtac tgcggccttg acggatgtta gtaaaccctg cgcataatct
    27061 gggttgtgaa taatagtgat attgctctgg ttcgcataac gttcgtgcag ttcattacca
    27121 cgatagccgg tgactaaaat aattcggcta caaaactgca acgcattttt gatacttgta
    27181 tcaagaattg ttccctgttc ccagggtaac atcattttcc attgccccat tcttgatgat
    27241 aatccggcag cggtaattat acagtcgatg gctgacatag tggttccatt atttataggg
    27301 gctaataacg cctgataatt gcgttacttc ctgatggatt aagttgggga taatttatcc
    27361 cagagaggtc ataaagactc gtgaaaagta tagttgaccc atcggcatta gtcattgatt
    27421 taggtgcgca gaaacgtccc actgtgattt ctgttgttgg tgctgggggg aaaaccagct
    27481 tgcttttttg gctggcagaa ctgctccagg ctagcggcag gcgtgtgtta atcacgacga
    27541 cgacacatat gtttatgcca acgtctcact ggcccgtggt tttctgtcgt gatcccgcca
    27601 tgcttcctca tgcgtctctt acatccccca tttcattttg ttttcacagc tggaaagcga
    27661 accagggaaa agtgcaggga tttacgccag aagcgattga tgcactggtg caacgaccag
    27721 agtgtgacgt aattctcatt gaagcagatg gctcgcgtgg aatgccgtta aaagcgcctg
    27781 atgagcacga accttgcata cccaaaagca gttgctgcgt gattgctgtg atgggaggac
    27841 atactttggg cgcgaaagtg agcacagaaa atgtccatcg ctggtcgcag tttgctgata
    27901 ttaccgggtt aacacctgat gcaaccttgc aactgagcga tctcgttgcg ctggttcgcc
    27961 accctcaggg ggcgtttaaa aacgtaccgc aaggttgtcg gcgagtctgg ttcattaacc
    28021 gtttttctca atgtgagaat gcgattgcac aaagcgagct ccttcaaccg ctgcaacaac
    28081 acgacgtaga ggcaatctgg ctgggcgata tacaagaaca tcctgcaatc gcgcgcagat
    28141 ttgtgaatta gcggcccggg tattccgggt tactgaaagg atatccattg aggttggtat
    28201 gaatattttc acagaggctg caaaactcga agagcaaaat tgtccgtttg cgatggcgca
    28261 aattgttgat agccgaggct cgactccccg ccattctgca caaatgttag tgcgcgccga
    28321 tggttctatc gtcggtacaa ttggtggcgg aatggttgag cggaaggtga ttgaagagtc
    28381 acttcaggca ttgcaggaac gtaagccgcg attattccat ggacgtatgg ctcgtaacgg
    28441 tgcggatgct gtcgggtcag attgtggagg tgcaatgtca gtatttatca gcgtccatgg
    28501 tatgcgtcca cgtctggtgt tgattggcgc ggggcatgtc aaccgggcga tagcccagag
    28561 tgcggcgcta ttaggatttg atattgccgt tgccgatatt tatcgcgaaa gcctcaatcc
    28621 tgaactattc cctccatcaa ccacgcttct ccatgctgag tcgtttggtg cagcagtgga
    28681 agcactggat attcgccctg ataattttgt cctgattgcc acgaataatc aggatcgtga
    28741 agccctcgac aaactcattg aacagcccat tgcatggttg gggttgctgg caagtcgccg
    28801 caaggttcag cttttcctgc gtcaattgcg tgagaaaggc gtggctgaag aacatattgc
    28861 ccgtttacat gcgcccgttg gttacaacat aggtgcggaa acgccgcagg agatcgccat
    28921 cagcgtgctg gcagaaatat tacaggtgaa aaataacgcg ccgggtgggc tgatgatgaa
    28981 accttctcat ccttccggac accagctggt ggtgattcgc ggtgcgggag atatcgccag
    29041 tggtgtggcg ctacgtctgt atcatgcggg ttttaaagtg atcatgctgg aagtggaaaa
    29101 accgacagtg attcgttgta ccgtggcgtt tgcccaggcc gtgttcgatg gcgaaatgac
    29161 ggtcgaaggc gttactgctc gcctggcaac cagctctgcg gaagcgatga aacttaccga
    29221 acgcggattc atccctgtga tggcagatcc cgcctgttca ttgcttgatg aactgaaacc
    29281 gctttgcgtg gtggacgcta ttctggcgaa acagaatttg ggaacgcggg cagatatggc
    29341 accagtaaca atcgcgcttg ggccgggctt tactgcaggg aaggattgtc atgcggtaat
    29401 tgaaacaaat cgcgggcact ggctcggtca ggtgatttac tctggttgtg cgcaggagaa
    29461 taccggtgtt cctggcaata ttatggggca taccacccga cgggtgatcc gtgctcctgc
    29521 tgcaggcatt atgcgatcca acgtgaaatt aggcgatctg gtgaaagagg gcgatgtgat
    29581 tgcctggatt ggtgagcatg aaattaaagc accgttgacg gggatggtgc gtggcttgtt
    29641 gaacgacggc ctggcagtgg tcggtggttt taaaattggt gatatcgatc ctcgtggtga
    29701 aacggctgat ttcaccagcg tttctgataa agcccgggcg attggcggcg gcgtacttga
    29761 ggcgttaatg atgttgatgc atcagggcgt gaaagcgaca aaagaagtgc tggaagtggc
    29821 ttaaatgaaa atggccggat ggtattaccc acccggcctt aaaattactg acaaatcacc
    29881 gttccggttt tgccttcaat cccctctttc gctttgctca atacggtaat cagtgcttca
    29941 cgacctgcgc gagagcgagc aaacgaggcg gcagcttcca ctttaggcag catagaacct
    30001 ttcgcaaaat gaccttcttc gataaagcgt tcagcatcac ttaatgacag gcgatcgagc
    30061 cactgttcat tctctttacc gaagttaatg gccacttttt ctaccgcagt cagaatgatc
    30121 agcatatcgg catcaatcat ttctgctaaa cgggcgctgg cccagtcttt atcgataacc
    30181 gcgctggtac cacgcagatg gttaccttca cgaataacag gaataccacc accgccaacg
    30241 gtgatcacca cctggcctgc atctaccaga gctttaaccg tttctttttc aataatatca
    30301 accggttttg gcgaggcaac aacacggcga tagccgcgac cagcatcttc tttcagagtg
    30361 taaccctgtt ttgtcagttg ctctgcttct tgctcggtaa agaacgaacc gatcggcttg
    30421 gtcgggttga ggaatgccgg atcgttagcg tcgacttcga cctgagtaac cagcgttgct
    30481 accggtttat taatgccccg agaaagcagt tcttctctta aggcgttttg tagatcgtaa
    30541 ccaatataac cctggcttaa ggcaacgcag acagacatcg gcagcattgg cgagtgcgct
    30601 tcagtttttg cagcagcttc aaaggcctgg ttaatcatgc ccacctgcgg cccgttacca
    30661 tgagtgacga taacttcatg tccttgggca attaaatcaa caattgcctg agaagtaatt
    30721 tttaccgctt tcatttgtcc ggccaggtca tcgcccagcg cattcccgcc cagggcgaga
    30781 acaattttct tactcatttt tcctcactca tcattcttga ttgtgtatag atccctgaca
    30841 gtggttagag cacgggaggg acaaacggtt tgcgtcgcag gaaacgacct cgcccggctt
    30901 tgcctgtaaa ggtgccgtca cagaaaatcg tttcaccacg ggataaggtt ctgacaatcg
    30961 cgccctgaca ggtaaaaccc tcccatggcg agtagtcggc gttgtcgtgg agatggcgat
    31021 gctgaatttg ttggctctga cgtgggtcga taatcaccac gtcgccatcg gaaccgggcg
    31081 ctaataatcc tttttgcggc cacaggccaa acagcctggc gggcattgcg ctggttaatt
    31141 caacaaagcg ttccggtgtt atacgtcccg tcatcacgcc actggaaaac agtaactgca
    31201 tgcggttttc cacaccgggt aagccatttg ggcagcgact gaaatcgcct ttagaaattt
    31261 gcaggcgttg agccatcgag aaggtgcagt gatcggtcgc caccacgtca atcgcaccat
    31321 cgctgatgcc acaccacagt ttgtcctgct cgcgtacgtt acgcagcggt gggctaagaa
    31381 tgaacttcat gccgtcttct gtatcgtaac tgcgttcgtc caacaggaga tattgtgggc
    31441 aggtttcaac ccagactggc tggtgattcg cacgggcaag acgcagataa tccagaccta
    31501 agccgttaga caggtgcacg atatagagcg gggcgttacc ggcaatttgt gccaggttaa
    31561 tcatgcgggc gatggcttcc gcttcgcatt ccagagggcg actcaaggca tgatagcgcg
    31621 gcgcggttaa ccctgcggcg ataaattccg cccgcttgct ggcgatagcc gcatcatttt
    31681 ccgggtgcac ggtggtcagc gcgccggatt catgcagacg gcgtaatgcc tgcaaaacct
    31741 cgtcatcgtt gagtttgtat tgataggtta aatagagttt aaaactgctc agtccttcct
    31801 cgaccatcat cgggatttcg tcgaggattg cgtgattaat gtgctggatc acaccgtgaa
    31861 agctgtaatc gatgaccgct ttatgggcgg cataaccacg ataaacctcc agttgatggc
    31921 gtaaccgaca gccgtttggg ccaaatccca tatggtcaat aatggttgtt gtaccgccac
    31981 acgcagctgc gcgggtaccg gtaaaaaaat catcacaact gcgcgcgatg ccgacatcaa
    32041 tattgaaatg cgtatggaca tccacgccgc cagggaaaac gtaacagcca gtggcatcaa
    32101 tttcttcata cgggagctgc ggcgaaatat tgttgcccaa ctggcgaaca atcccgcttt
    32161 caatcagcaa atcctgtttg gcttgtccat ctgcgttaac gacagtgccg tttttgatca
    32221 atacgcgcat agcaaactcc aaaaaccctc cggcgaaccg gaggggaata agtgaagagt
    32281 aattactctg ttgccagcca gcttaacggg attgcagcgt acatcgcggc acaggtcacc
    32341 aggtgagatt tccaggtttt ttcgttaggt gcatgcgctt caggttcttt acccgggcca
    32401 aagccgatca ccggaatgcc gtgacggccc atgatagata cgccgttagt tgagaaggtc
    32461 cacttatcaa caaccggcgc tttgccaaac agcccttcgt aggcattcac cagtgcttta
    32521 acggtgaagt gatcttcttc cactttccag gtcgggaagt agcattcggt tgggtaaacc
    32581 aggccagtcc aggacggacg gtcgtagttg tacatagaaa caaccgcgtt agctttctgt
    32641 actgcaggca gggcgcggat ttcgtccagc gcgccttccc aggtttcgcc ccaggtcaga
    32701 cggcggtcaa tagagacggc gcagctatct gctacagcgc aacggcttgg ggatgtgaag
    32761 aagatttcag aaacggtgag ggtgcctttg ccgaggaatt cgtcataacc cagacgttgg
    32821 gagagttctt gtaattcgcc aagaatcgga cccattttga aaatggcgtt gtcaccgcgt
    32881 tctggcgcag aaccgtggca gctaacaccc tgaacatcaa tacgaatttc catacgaccg
    32941 cgctgaccac ggtatacctg gcagtcggtt ggttcggtac tgaccacaaa ttccgggcga
    33001 atgccggatt gttcaataat gtactgccag cacagaccgt cgcagtcttc ttcctgcaca
    33061 gtaccggtaa ccagcagggt atattcatct tccagaccga ggtctttaat gattttaccg
    33121 gcataaacca tagatgccat gccgccttcc tggtcggaag taccgcgacc accgatcagc
    33181 tcatcagttt ccatgccttc gtacggatcg aagtcccagt ttttgatgtt gccaatgccg
    33241 acggtatcga tatgagcgtc cattgccacc agacgcgggc cgtggccgat ataaccgaga
    33301 acgttgccca tcgggtcgat ttcaacttta tcgaagccga ctttttccat ctcttcttta
    33361 atacgatgta ctacgcgttt ctcgtcgcag ctttcactgg gaatagcaac catgtcgcgc
    33421 aggaagcgag tcatatccgc ctggtaatct tttgcttttt caagaatcag tttgaatgga
    33481 atattcttag ccattatttt tctccagatt ttttactccc cgttttgcag ggagcagttc
    33541 caatagggtg attaaggtgc tacagcgtgt ttgccttccc agacaacttc gcggtagtgc
    33601 ttcacgtcgg tgtcgccttc agtgctgata accagcacca cggcatcttt gttcagcgcc
    33661 agtttttcca tcaggctttg acgttgcggg tgataatgaa ccgctgcgag aacgcccaaa
    33721 ccgacagcgc cggattcacc ggagatgatg cgcgggtcgt tgccgtacgg attacccagc
    33781 acgcgcatac ctaatgcggc aacgctgtcc tggcaggaga tgaattgggt ggcgcagtta
    33841 cgtaggattt cccagcccag cgggttaggt tcgccacagg ccaggcctgc catgatggtg
    33901 gccatatcac cgccaacgtt gacgatgtcg cctttgacgc cggagcgata aatacagtca
    33961 gctttgtcag gttcaacaat aatgctgtgc agattttgcg ggctatagac gtcgaccaga
    34021 taacccagca caccaccggc cattgctccg acaccggctt gcagcagaac gtgcgtcggg
    34081 gttacgccca tttcacgcat ttgctcgacg gcttcatctg ccagggttgc gtagccttgc
    34141 atgatccagg ttgggatttt ggtgtaacct tcccatgccg tgtcctgtac cacttcccag
    34201 ccgtgctgct gcgcgtgttg catggtcagg cgaacggtat cgtcatagtt catatctgtg
    34261 acgatgcact cggcaccgag gttcagaatg gcgtcaacgc gttcctgagc agaacctttc
    34321 ggcatgtaaa tcaccgcatt ctgtccgagt tgctgcgctg cccacgccac accgcgcccg
    34381 tggttgccgt cggtggtggt cgcgaaagtc attttttcgc cgatggcatt tttcaggtgc
    34441 tcaaatgaca gcgtttcgat atcaagatga tatttttcac acaataattg agcgatagcg
    34501 tacgcaccgc cgagcatttt gaaggcgttc agaccgaatc gttttgattc gtctttgaca
    34561 agaattttct tcaccccaaa aaggtttgcg agatcgtcca gcgcacaaag cggtgttggg
    34621 cgataaccag ctattttctg gtggaactgg cgcgccagtt tggcctggct ttgcgaaaag
    34681 agcggtgatg tttcgccgtt gaaaaatttg ttatcggcga tatcaatctt caatgagaaa
    34741 acggacatat cctatccttt tggcctctgg aaatagaggg ccgaccagct gatcggcccg
    34801 aggggttatt tcacgcgttc ttgcgcttct ttcagcagtt gctcgagcag tgcacctggt
    34861 ttggcgtatt tacgggacag gatcatcgcg gcgatgatat aaggcttcca gctggcttct
    34921 ttgtaggtag cgatacggta tttttcgaat acgccttcag tcacttcacc ttctttacag
    34981 gatacgccgc tgatatcagc tggcaggcag tgcatgtaca gggcttcgcc atcacgggtc
    35041 agttccatca tctcttcagt acaatgccag tctttgtgtt gcgcgttctg tgccagacac
    35101 tgtttttcca gtgcttttaa gccttcgtga tcgttagcgc gcagcaattc ggtacgctgt
    35161 tccatcactt tgtaaggtgc ccatgacttc ggataaacga tgtctgcatc tttgaacgct
    35221 tcttccatgc tggtgacctg acggaagcta ccgccagatg ctttagcatt gtttttcgcc
    35281 acttcaacca catccgggat caggtcgtag ccttccggat gggccagggt gacatccata
    35341 ccgaagcgag tcatcagacc gatgatgcct tgtggtacag agagcggttt gccatagctt
    35401 ggagagtagg cccaggtcat ggcgattttt ttacctttca ggttttccag tgaaccaaag
    35461 tgttcacgta accacgcgag gtcagccatt gactgagtcg ggtggtcaat atcgcattgc
    35521 aggttcacta aagccggacg ctgtggcagt acaccctgct tgtaaccgtc atcaagtgca
    35581 gcgccaactt cacgcatata ggcgttgcct gcgcccagat acatatcgtc gcgaatacca
    35641 atagcgtcgg cgcagaagga gatcatattg gcggtttcac gcacggtttc gccgtgagcg
    35701 atttgtgatt tgccttcgtc gagatcttgt tgtgcaaggc cgagcaggtt aagcgcggaa
    35761 gcataagaga agcgggtacg ggtggagttg tcgcggaata cggaaatacc taatccacta
    35821 ttaaagactt tggttgagat gttttcagca cgcagtgctt ttaatgctgc ggcaacgtcc
    35881 agtacttgtt tcagttcatc tggcgtctgt tcccacgtta acaaaaaatc tttctcgtga
    35941 aggtgagagg tcagcgaatt gatatcctta atcagctcat taacagtttt catcataaag
    36001 tcccggtagt ggaatgacag gcaaagtggt ttatttcact gtgacttgcc aaagtgattg
    36061 gtgttttcac agctatacct gggactaaca ataagtgtgc cagaattatc cacggggctg
    36121 caaaagacta atttgacgga taatgtgtgg ggttgatcac aaattgatgc cagcttccgg
    36181 ttgtttgtaa aatacgaaaa cgtttggctt tttttatcgc gttatttatt tgctgctaag
    36241 tatcagaaat aagataaagg gacaggctat ctctcttcag tttgataaac tcaccgccgt
    36301 aattctcata ttgatataga aatgtgatat ctcttccata atttcattag aaaatgataa
    36361 aaatcttatg tttttatcaa agccaatttc gcctgagtta atatcatcat gatctgtgaa
    36421 ccatcaacgt cttcaatgat cgacttttaa gtaagtctgg aaggtaaagc tatggagctt
    36481 gctactacgc agtcagtatt gatgcaaatt caaccgacaa ttcagcgttt tgccagaatg
    36541 cttgccagcg ttttgcagct tgaggttgag atcgttgatg aaaacttgtg ttgcgtcgcc
    36601 ggaacgggcg cgtatgggaa gtttcttggt cgccagttga gcggcaactc acgcctgctc
    36661 cgccacgtcc tggaaacgaa aactgaaaaa gttgtgacac agtctcgctt cgatcccctt
    36721 tgcgaaggtt gcgatagtaa agaaaattgc cgtgaaaaag catttctggg tacgcctgtc
    36781 attttacagg atcgttgtgt tggggtgata agtttgattg ccgttaccca cgagcaacaa
    36841 gagcatatca gtgataattt acgcgaattt tctgattatg ttcgccatat atccaccatt
    36901 tttgtttcga aacttctgga ggatcagggg ccaggagata acatcagtaa aatattcgcg
    36961 accatgatcg ataatatgga tcagggcgta ttagttgttg atgatgaaag tcgggttcag
    37021 tttgttaatc agactgcctt aaaaacactt ggtgttgtac aaaataatat tattgggaaa
    37081 cctatccgtt tcagaccatt aacatttgag agtaatttta ctcatggaca tatgcagcat
    37141 attgtttcgt gggacgataa aagtgaatta atcattggtc aattgcataa cattcagggc
    37201 cgacaattat ttttaatggc atttcaccaa tcgcatacca gtttttctgt agcaaatgca
    37261 cctgatgaac cacatattga acaattggtt ggcgagtgcc gtgttatgcg gcaattaaaa
    37321 cgactcatta gccgtattgc acccagccca tccagcgtta tggtggttgg tgaaagcggc
    37381 acgggtaaag aagtcgtcgc ccgagcaatc cataagttga gcggaagacg gaataaaccc
    37441 tttattgcta tcaactgtgc cgcgattccg gagcagcttc tggaaagcga actgttcggt
    37501 tatgttaaag gcgcatttac tggcgcttct gccaacggta aaacagggtt gattcaggcg
    37561 gcgaatacgg gcacgctgtt tctcgatgaa ataggtgata tgccattaat gttgcaggct
    37621 aaattactgc gcgctattga ggcgcgtgaa attctgccga ttggtgccag tagcccaata
    37681 caagtcgaca ttcgcatcat ttctgcaact aatcagaatt tggcccagtt cattgccgaa
    37741 ggtaaattcc gcgaagatct cttctaccga cttaatgtta tcccgataac tctgccaccg
    37801 ctgcgtgaac gtcaggaaga tattgaacta ttggtgcatt actttttaca tctgcatacc
    37861 cgtcgtctgg gatcggttta tcctggcatt gctcccgatg tcgtcgaaat attgcgtaag
    37921 catcgttggc ccggaaacct gcgcgagtta agcaatttga tggaatatct ggttaacgta
    37981 gttccttcag gtgaagttat cgacagcacg ctattgccgc caaatctgct gaataatggc
    38041 acaacggagc aaagtgatgt aacagaggtc agtgaggcgc acttgtcact cgatgatgcg
    38101 ggcggcacgg cgctggagga gatggaaaag caaatgatcc gcgaggcgct ttcacgtcat
    38161 aacagcaaga agcaagttgc tgatgaactg ggcatcggca ttgctacgct ctatcgcaag
    38221 attaagaaat atgagttgtt aaacacataa cctcagtgaa tccggcctga attcaggccg
    38281 gatatccttt tacttcgttt tctcgcaatc cagaactgta tttacaatca tctgataccc
    38341 cgtgcagcga caaagatttc ccgccagtcc gcgacgaatt tccgtaatgg ttaatggctt
    38401 ctcgcgtggt ttcgccagca ttgccgtggt agccataatc aggccaggcg tacaaaaccc
    38461 gcactgcact gcgccggatt tcgcataagc ctgctgaaca tgagaaagtt ttccgccttt
    38521 cgcttcacct tccagcgtgc ggatctcttt tccttcagcc caggcggcaa ggtataagca
    38581 actgtctatt gctgtgccgt cgaccaacac cgtacaggca ccacattcac ccacgcagca
    38641 cccttgtttg acacttagca gtccttgttc gcggagtaat tccgagagcg gcgtgcctgg
    38701 tgcggcgtga agctgaaaag gcatcccgtt aatggtgcat tcgatggtaa ttgtttcgct
    38761 gtgattcatt gcaattttcc ccccgccgcg gcgacggctt cgctaatcac ttttttggtc
    38821 atcgtctgga tgagatgcag acgaaactct ttactggccc gccatgaaga acgcggggcg
    38881 acatcttgca ggacagattc gctgatagct tccagcgttt gcaggtttaa tggcgcattt
    38941 tgtgcagtct gttcggcatg ttggcagcga atcggcgttg gcgcggcaac accaaatgcc
    39001 aggcgtaatt cgctgaaatt gccgttatcc agtcggcaat gtgcggcgca gccaatcgtt
    39061 gaaatatcca ttgcgtcgcg catggcatat ttaaaatgcg cgctgcccgc gtgttctttc
    39121 ggctgtggcg gaaaatgaaa ggcgacgagg atttcgtcat gctcaagaga cactttgccc
    39181 ggcccggtgt gaaagccatt aatcgggacg aaacgaacac cgcgcgggga gtggatctcc
    39241 agtttcgcgt cataaattag cgttggcgtg gcagaatctg cgctggtggc accgttgcaa
    39301 atatttccac cgtaggtagc gacgttacgg atctgcggtc cagcaatgga cgtggccgca
    39361 gcacataacg ccgggagatg acgttgagtt ataggatctt ctattagctg ggtaaatgtc
    39421 gttgcagagc cgatacgtag cgagccatct tccgccagcg taattccccg cagctccgcc
    39481 agattatgaa tatcaacaat atggcgataa cggtcattgt gatggtggag ctgaatcagt
    39541 acgtcagtgc caccggcgag cagtttggcc tgcgggttgt cagccagcag gttgatggca
    39601 tcggcaaggg ttgctgcgcg atggtaagaa gcaaaatcaa acatgatgtt atcctcaaat
    39661 caatcctgcc agatggaact cttcatataa ccgttttggc gtcagcggca gtgtattgat
    39721 tgcaacaccg gtagccatct tcaccgcgtt acgaatagca gcggcaacag gaattattgg
    39781 tggctcaccc agtgacttat gtccgtatgc ggattgcggc tcattgattt cgacgaacgc
    39841 gctttccagt tgtggcagat ccggcatggt cggcattttg taatccagca gattggggtt
    39901 acggaccacg ccgcttttag catcgatgat catctcttca aatagcgccc agccaatgcc
    39961 cattcccatt ccgccgtgta cctgaccttc tgccagcagt ggattaagaa tatgccctga
    40021 atcatgaacg ttgaggatgc ggttgatggt gactttgcac agcgcaatat cgaccgtcag
    40081 atcaacaaag gtacagccaa acgccggtgg gttagtggtg gttttgatgg agctttcagc
    40141 agagagctgc ccgccgcgtt cagggtggta gaaagcgtcc atcgccaaat cttttaacga
    40201 cattaacggc tcttccggtc gttcaaccag cacgatatgg ccttttatca gggtcagatt
    40261 catcgctgac tgatgtagca tgactgcggc gtgagcgatg attttctctt ttaataatag
    40321 tgccgcactg cgcagcgcag gcgcggcaac atagctctgg cgtgaggcaa atgcgccggg
    40381 atcgaacggc gtaacgtcgg tatcttgagt tgaaataacg cgaacgtcgc tgaccggaac
    40441 ccccacggtt tctgccacca tttgcgagaa gacggtgtcg gcaccctgac cgatttccgt
    40501 cgcgccgctt tgcacgttga tggttccatc ctgattcatc agaaggcgcg cgcctgctat
    40561 ttctacgccg acaggccagg tgttagaggt gtagctaaaa caggcgacgc caacgccgcg
    40621 gcgcaaattg ccttgctggt tctggcattc tgcacggcgt ttttcccatt caaagatttt
    40681 ccggcctttt tcaagacact ccggcaaccc tgcgctgtaa atacgtttgc ccgtgagcgg
    40741 attagcatct ccttcgcggg cggcgttgcg taaacgaatt tcaacaggat caatacctaa
    40801 cgctgtcgcg gcgtcatcaa gcatagactc aacggcaaat acgacttgtg gcgcgccata
    40861 accacgcatc gcaccagccg aggggaggtt ggtatagcag gtctttgaac tgtaagcgta
    40921 ggcacaacga ggataaaggt aagcgacttt attccccccc gcagaagcga tggagtgccc
    40981 gtgagatgca taagcgccgg tgttagacag aacatccaga ctataacctt tcaatgttcc
    41041 gtcgcggttc acgcccattt gcccgtcaat ggtaaaagcg tggcgggtac gggttgcgag
    41101 gaaacactct tcacggctaa gggaaacttt caccggaatg ccgccaagct tgctggtcag
    41161 gaatgccgcc attggctctt ccagtacatc ctgtttatta ccaaaaccgc caccgacaaa
    41221 cggtttgatg actcgtacgc atgaccaggg aatatccagc gcctgaccaa ccacgcggcg
    41281 aacaatgtgc gggatctggg tgctggaaac gatggtaatt cgcgagtcat cctccatcca
    41341 cgccagcgat gttacgcttt ccatgtgaca atgttgaata acgggggtct gatagtgccc
    41401 ctgtacctgg tagtcggcgg catcgattgt ttgttggaca ttacccgtcg acatcgtgct
    41461 ttgtttcagt aaattgccac cgttatggat tggtgcagcg tcttctgcca gcgccgcttc
    41521 tggcgtggtg ataacgggta attcttgcca ctcaatgctg accaattgcg ccgctttttc
    41581 tgccgtgagt tcatcgcggg ccacgacgat ggcaacggcg tcaccatgat gacgaacatg
    41641 gcgagttagc agtgcgcgat cggcggtatc gcgcttgttt tcgtcaagtg tccaggcatg
    41701 cccagcagta gcaaatggaa tatcaggcac atcttcccag gtaaaaatcg ccagtacgcc
    41761 cggcaaactt ctggcttgtt catcattaat acttacggca taaccatgtg cgatagggct
    41821 acgtacatat ttcgcataac acatgcccgc cataacataa tcgtcagtat atcgtgcccg
    41881 cccggtgacc ttagcaatgg catcgacgcg catgcatgat tcacccgtag cggttgcttc
    41941 ccgcgcttcc atagaaatcc cctcgatttg aacatttggt tcttatggca aagatttagt
    42001 ctaagcaggc aagatttacg ccagaaacga cgataagcgt taggggaggt aggaagtgtg
    42061 atgacgttaa aaaaacacag atatgatacg aaacaggact ctgaataggg gcagtgatac
    42121 gtatcaatat gagtggcgat aaaatcaatg tgataaattg ggattattac gcatcaggat
    42181 gggaaatagg gtaaaaagtc aggaagttcg atacctctac agcgaatatt ttgctgatga
    42241 tacttccctt tgccaaatga aattaacctc tttctctatt accacgtttt tccagaagca
    42301 agagattggg tatcaagagg ctggctgcta tgataaggcg tcttgttttt taagcgagga
    42361 aagattttga gtgcgggacg cctgaataaa aaatctctgg gtatcgtgat gttgttatcg
    42421 gttggactgc ttttggcggg ctgttcgggt agcaaatcat ccgatacagg aacgtattcc
    42481 ggctccgttt acaccgtgaa acggggggat acgctatatc gtatttcgcg caccacggga
    42541 accagcgtaa aagaactggc gcgactgaac ggcatttccc ccccttacac cattgaagtt
    42601 ggtcagaaac taaaactggg tggggcgaaa agtagcagta gtacacgtaa atcaaccgcc
    42661 aaatcaacga ccaaaaccgc atcggttaca ccgtcatcag cggtaccgaa atcttcctgg
    42721 ccgccagtag ggcaacgttg ttggttatgg ccaacgacag ggaaagttat catgccgtat
    42781 tcgacagcag atggcggcaa taaagggatt gatatctcag ctccacgggg tacacctatt
    42841 tacgccgcgg gtgcaggaaa ggtggtgtat gtgggcaacc agctgcgtgg ctacggtaat
    42901 ctcatcatga ttaaacacag tgaagattac attacggctt acgcccataa tgacacgatg
    42961 ctggtaaata atgggcaaag cgtgaaggct gggcaaaaaa tcgccactat ggggagcacg
    43021 gatgcggcat ctgttcgcct gcatttccag attcgttacc gtgcaacggc aattgatccg
    43081 ctacgttact tgccgccgca gggcagcaag ccaaaatgct gatggcgaat taatcagcag
    43141 tcagcagcgc ggcacttgct aaggagagcg taaggtttat aatgccttac gcatctcgaa
    43201 gcgggcgtag ttcaatggta gaacgagagc ttcccaagct ctatacgagg gttcgattcc
    43261 cttcgcccgc tccatgacac ttcttaaagt ctactaactt actgaatcct aagggatacg
    43321 gcttaacgcc ttccgatagt tttactcact gtaaccaaca ctgtgactaa ctttgttgaa
    43381 gtagtgagca gacctaaggt ctcagtacat gctcaagtca agaacctacc tttatcagcg
    43441 taatggtgtc ttttacatcc gtcttaggat gaagaccact agtcgcctga ccgcttcgct
    43501 tccttctcac aaccgttata aactagcgtc agtatcttta cggaccaagg atagacgtac
    43561 cgctatggct cactcacggc acatcaagtc agcacttaaa gcaatccatg cagacaaccc
    43621 taacgcctct tatgaggagc tacgggagca cctgaagacc attgtcgagt gggaacttag
    43681 tgtcagccgt gatgacctga acgacccgga gtcctaccag ctttacgttg accagtacga
    43741 tgacattaag tccaaccttc gggaagctgt agcgaccgag cgtctgactg tagaccagca
    43801 tcgctatatt aatgacgtta tcggtgtgct taaggcctgt caggatagac tcaatggcga
    43861 tagctctggc ctattgtctt accttgagcc tgagactggc agtctaagac cgtccgtctc
    43921 cctatctgta ttggcagaac ctgaagtccc tgaacctaaa gcactgaccc tcgcgtctct
    43981 cattgagcag tacgagcagg agaacgccca gaactggaag ccagcgaccc taagcgaaaa
    44041 ccgagcgtca cactctacgc taatcgagat attcgactat ctggatattc aggatgtcgg
    44101 caaggcgacc cgtgcagata tgctcagagt tcgtgaagtc ctccagcagc taccgaagaa
    44161 ccgcaagcaa cgctttaagt ctatgccgct gtctgacttg ctgaaccggg agtctaagac
    44221 tgactgtctg gacgtcgtta ccattaacaa taaatatctt atcaagatgg ctgctgtgtt
    44281 taagtgggcg gtacgcaacg acttgatagc taagaacctg accgaaggtc ttgagctgaa
    44341 agtccctcag cgtaaagcct ccgacgctcg tgatgcgttc agtcctgagc aggtagggca
    44401 actactggtc gcagctaaag cgtactcgca gaagacctca ggtaagccat accattatta
    44461 cgtcaccgct ctggctgcga ttacaggtgc gagacttaac gaggtggccc aacttcaggt
    44521 taaagatgtc aggaccactg aggctgggac cgtttttatc catatcaacg aagacgatag
    44581 cagcctgccg ggtaagagta tcaagaacgc ccacagtgac cgctgtgtcc cgttggtaga
    44641 tggtgcttat ggttttgtat tggctgactt tatgtcactc gtggaagacc gtagaaagac
    44701 tgagggtgat aatgctatgg tgttcaatgg cctcaagcta atgaagaacg gctacggtga
    44761 gcaggttagc aagtggttta accgaacgtt gctgcccaaa gtcctcgctg accgtagcgg
    44821 cttagctttt cactcgttta gacatacagt tgcgacccag ttaaaacaac atggggtcga
    44881 gttagcgtat gctcaggcga ttatgggtca tagctcaggg tctataactt acgaccgtta
    44941 cgcgaaagag gtcgaggtgg aaacgctgaa agaaaaatta gcggagtcat tgtcagtaaa
    45001 aaaatagacg gaaagtaatc gcatagggat agcggaacgt gaggacgtaa tggtcaaaga
    45061 atttttagtt agcaaagaaa ataagccttc atatgaatgt atgaatactg ctgaaacttg
    45121 tttggggctg gctggaatgg cttcggaaga ttggcaacgt gagcatcatt gccgcacagg
    45181 tataatattt actgcttttg ctattgaggc aatgttcatc ttctatagaa aacaggttga
    45241 ccccggctac gataagacgc agaaagagtg cagaaagaca atgcacaaga atacgttaaa
    45301 gttgtgcggt ataaacaatt atatggggac aaaaccttac caaattatca aagagtgtct
    45361 tgaggtaaga gacgctattg ctcacggcga ttcgtatact tcgagcttta atttctcggc
    45421 ggatcatttg gataaccaag acgatattgc aaaaagtgtc ctggcggtaa actctgaaca
    45481 atttcgggga ttaactgttg agcgccttgc aaaatacatt gaaatggcaa agcatattga
    45541 ccatgagatt tctgataatg gttacaggcc ttctgaaggt taccttccag cagccgagcg
    45601 cagaagtctg atgttagctt ttggcgtcag tggtgtgtca atgtggccca gtgcttgagc
    45661 gataatacac ctcactatag aggaatggac ccttaactat cactataggg gagagaccct
    45721 aagtccccac cttaatagca gtcacgaccc atacacacat tcattaagtc tcaggcttcc
    45781 ggtagcggac cgattggaaa ccatccgcag acgcctggtg ttaaatcacc ttggcctgag
    45841 accattcctt ttaacgtaag ctatcttccc ttttgacagg cggggagcag tagaccacat
    45901 ccgactttaa gttggctctg tgataatcgc tgcgcggata actgaagaac caagacacca
    45961 ttactgaggt ctgccatcca tttgtggaca ggcggaaact caaagaggtt agatcagtgg
    46021 tgtctgaggt cggagctttg agtcctgacc aggcaggctt gaggaggcca tgagttcatc
    46081 ttgttatgga ttgtgttatc acaattccaa taagaagaac tttaagtcca gcctccagtc
    46141 ctgtcagccc gaagtatact acggtcgcta tgatgtggtt ctccatactt cgggtctaac
    46201 tggaggtcct gaacactttc gagtcaagtt ctccgaagtt cattactgtt tgactaaaat
    46261 atcactatag gaatgacacc gagtaaataa ctatttctgc ctctggcctt gatgcgtcaa
    46321 gtcatgcgga aatattcttc tgtgtcttcc actttaatta cgtggtcatc cccgaccaat
    46381 caatccaatt cgctttcgta agcactttcg gcggctccac ttggttgcgc tgaattggtg
    46441 tggatacccg caggggacga gagtgcttaa ctaaaggagc agcctttatt atgtctatca
    46501 aattaacgac catgcaagcc gcttacctta ctcgtgcaat gactacaggc ggtggattaa
    46561 ttgagcttgg agaattggct aagggattgg gtaaacagaa agcccatgtg gtccgtaagc
    46621 tggaagctaa ttttgacaaa gagcggcttt ccaaaatgga aacccactta accgtaatgg
    46681 gtggcaacca taagaatgta gcccagaccg tcaaaaccta tatgctcgac tacaagactg
    46741 ctggcgctct ggcgatgagc tacgatcttc agttaggtgt agaagttctc acgcttctag
    46801 aggaggccgt tcagactatc ggcgctatga ccggggccgt gcaagcagga gatacacaag
    46861 cagcacttga ggcgtcatta ttgttctctg agcgtatgcg taaccgtctg gaatatacgt
    46921 ccggtgagac agaaaatgaa acccgtagcg tagctttgaa gcgtctggga cgtaaaggag
    46981 ctagcgtatg attgacccgc gtactcccga gggacgtttg accctacgtt accgtggtct
    47041 ccgtacttca ctacttttgt cgatgcttgg tttggataaa gacgccacgg ataaccgacc
    47101 gttttattct cgtaatgagc taatagaaag attggttatc cgtgatatgg aatttaaccg
    47161 aggaaataag taaggaatcc tctggtcact gcgtcagtaa atcccacaag taatgcgata
    47221 gccgaaggga tgatattaat tgctgcgaca gaaagtgatt ttattattct ctgttctttg
    47281 gatgccttta ttaaaaccgc aaagataaac actggaatga atgcaagtag ggctatggtg
    47341 attatacgtt caagcatata cagtgtgtgc caagctatcc cacctgttat atatccagcg
    47401 ccgtatccaa ctgcataaaa gcaggccaag taaagcaata agctaatagc taatttgggc
    47461 ataaatcatt cctgagtgtc ttggtgaaag atgggcgtag cttagcaaat agccatatcc
    47521 ctattgatcg tttgttccaa tcataatgtc ttcaattgat cgttcagagt gatcaaacct
    47581 aaccctattt aaccttttga gatgaccgat gcggggagat gccgcaggac taaacacgcc
    47641 tgtggtcgac cgatagtctc aaccctcaag aggagtacca acttatgtta ttcaacaaca
    47701 acgactggaa actatcagtc accgacatca atccctacga aaatactgtg agtctggacg
    47761 gtcagtccta tccgctgtct ctggcgatta agaccctgat tccgggctac ctgtcaggct
    47821 taccgtccac cagccgcgag gcgatggagt tactggaagc tctggctgaa gctggcgtga
    47881 ccattggtaa cttcttcagt aatgacctga tgaccgccta tggtcgccgc cagcagaaca
    47941 agcgagtcga agccgagcgt atcgctaaag agcagcgcat tcaggcggac cgtatgcgtg
    48001 aagagaacat gaccgacgaa gagtggcaga aagagctaca gcgtcgcgag caggtcaaag
    48061 ctgaacgccg gacctatggt gagaacctcc gcagcgctac tcattccgct ggtcgctcac
    48121 gtgccgctat cgtcgctgac cttgagtcag gcggtaactg gatggacagc ttgtaagcaa
    48181 gcgtccctca tctgagaaca cacggtccct gactgcctgt tagggacttc aagtatttga
    48241 ctcaaggaga ttaattataa tgaatatgac taaaaacgct ttccagtttg gcattgagcc
    48301 tgttcatatc accgacaacg acaaccttca ggttaatgag ggccttccga ctaacgccga
    48361 ccctcaggtc tatgccctcc agttagctaa gactgttaag gctatgctga acggtgtact
    48421 taaagacgct caagataaca tcccgttccc tgtagaagtc ctgccgaccc gtaacagctt
    48481 accgactcca atcattgctc acactctggc tgaccgctct gtgctggtcc ctgtccgtgg
    48541 tggtaagcga cctgaagttg tgaccgctcc gtctggcact gaaatcaccg ttgagccgat
    48601 tgaacaggcg atcctcgtgt ctcaccaaac aaagctttgg gaccagaagt cgaccactgg
    48661 tttcactcag gggacgctcc agcaggacgc actgaacatc tgcgataacg tgattcgtac
    48721 cattaactct aagatggttg acgtgctgga gtcctcaaag ctgctgaaga ccgtagaact
    48781 tccggccctg actggtagcc tgaccgctaa ggctgacgct atcatggata ccctgtacga
    48841 gaacacggag tcttctttcg gttctgaggt ttctgactac ggcattatcg cccatgagtc
    48901 acacctcaag gctctctccc gtctggctgc taagcagggc ttcggtggtg aagacgctat
    48961 cgtcgatatg ctggggactg acgtcgccta ttacaacggt gaagaccgtg gcgtgttcat
    49021 gatggctaag cgttttacgg ctctgagctt cggttgcttc cgtcacgatg gcgagagcat
    49081 tactgtggtc ctttcacgtg atggagactc ccagtctcac gaccttgaga ttctcggcaa
    49141 ggtgtttgtt gtagctgaag ctgctacgac catcaagatg ggtactggct cggctactgc
    49201 tgtcctgcct gtagtcaaac gcctgagctt cactaaagaa gctaactaac aataacgact
    49261 attggtcaga cttccgaact ggatgtctgg cctttatttt gtactagtaa tctagttcgt
    49321 gaatacagaa agtaggaggg ccaaatggac ctacacaaag tctatctcgt ggtcttcctg
    49381 ctaaagctgg gactggtggt ctggctatgg gctattagac gatgagtttt gactgaaaaa
    49441 tgtgagacct catctaatat aaagagcgag ccaaaagccc ccatagcctg acctgagcca
    49501 gaccaaaagg gccgggtagt cagaccgtga gttctattcc tttagaggga atattacgtg
    49561 cttcaagtct tgaccttcta tctctaaatg tgtgactatc actacaacca actggattca
    49621 cctgtcttta agaaacaata ggttagctgg ttgtcttcga tttgtcgtag gctcccttcg
    49681 cccgctccaa tttatcttca cctgatcaat ctgccatttt tgctgtatct gattttctat
    49741 attatcttaa ttcaatcgct tcagactttt cgcaatcgcc gtaatacttc cccattttgt
    49801 tgtaaataaa aagcgtttac gcaatgcaat ccttttgtaa caacccaggt aaaacacctt
    49861 attttcaagt tgttaaataa gtggttatct acgcttttaa caaacgattg atggaatatt
    49921 ttatgaaagg gaaatctgca ttaactcttc tgctcgctgg tatattttcg tgcggcacat
    49981 gccaggcgac aggggcagaa gtgacgagtg aatctgtatt taacattctt aattccacgg
    50041 gggcagcaac agataaaagt tatttatcgt taaatccaga taagtatcct aattatcgtc
    50101 ttttgattca ctcagccaaa ttacaaaatg aaataaagag ccactatacc aaagacgaaa
    50161 ttcaaggatt gctaacatta acagaaaaca cacgaaagtt aacgttgacg gagaaaccct
    50221 ggggaacgtt tattcttgct tctactttcg aagatgacaa aacagcggca gaaacccact
    50281 atgatgctgt ctggctgagg gatagtttat ggggctatat ggcattagta tcagatcagg
    50341 gaaatagtgt agccgcaaaa aaggttctgc tcaccctgtg ggattatatg tctacgcccg
    50401 accagattaa gcggatgcag gacgtcatta gcaaccccaa acgactggat ggagttcccg
    50461 ggcaaatgaa tgttgtgcat atccgatttg acagcaactc ccccgtgatg gcagatgtgc
    50521 aggaagaagg caagccgcag ctgtggaacc ataaacaaaa tgatgcgtta ggtctttatc
    50581 ttgatctcct tatacaagca atcgacactg atacaattaa cgctgaagat tggcaaaaag
    50641 gtgaccgatt aaaatcggtt gcgctgctga ttgcttatct ggataaagcc aatttctatg
    50701 ttatggaaga ttccggagca tgggaagaag atgcccgatt aaacacatct tccgttgcac
    50761 tggtgacctc tggactggaa cgtttgtcaa acctactctc gaagaaagac tctgtttttg
    50821 tctctgattt actacgcgaa gctaaagtca atgaactgga tgaaactctt tcaacaacga
    50881 ggttgaacca cctcatagat aatggttatg aaagaataac gttacagctt gatttagggg
    50941 gagaatcccc aggttatctg gaaaaagata aacattacag agaagccgat gcggctttac
    51001 ttaatgttat ttatcctaca aatctttcaa agataaacac cagaagaaaa gagcaggttc
    51061 taaaaatagt taaaaaacta gcaggtccgt atggtataaa aagatacgag aaggataatt
    51121 atcagtcggc aaatttttgg ttcaatgata ttaaaaccga taccgatcaa aattcgcatg
    51181 caaaaagaga aaagagcttc attccatcaa cagaagcaga atggtttttt gactcatggt
    51241 atgcaaaatc agcagcaatt gtttataaag aatcaagaaa agaagagtat ttgaatgact
    51301 cagtacaatt tatgaataga tcattggccc aaataacagg cgagaatatg attggtgcaa
    51361 atggcaggag cgtaccagaa atggcacttc ccgaaagcta taactatatc cacaagtcgg
    51421 gaacttgcat gaagcgccaa gccctatcat tccgctaaat tggtcgaaag cttcaatgac
    51481 tctcatgttg aaagagatgt ccaaccttat caatgatgag ggaattaaat agttttcata
    51541 atgagttaca gcacaacgtt gggtattgac tgagcaagat gaacgacaag gacaacgaca
    51601 tcagccttat gaggagcaag aaaatgaaag caaattcgaa aacgatcaga aggatgaaag
    51661 ttaatgttcg gtctgaggaa agtaaatcga aacactcata cgtttgaaac cacttttcaa
    51721 aattggaaag aaaatggcga agatgttgca ttattaatgc ccgaatttag tgctaaatgg
    51781 ctaccgatag cagaagagag tggcagctgg tctggatggg tattgcttgg agaaatattt
    51841 cccttaattt catctgagct cgctgggatg gctttaatgc cagaaacgga aaggttaata
    51901 ggggaatggc tcagtttatc cagttccccg ttaaatctaa agcatcctga actaaaatat
    51961 aatcgtttgc gcgtaggtaa agtatttgat ggagtattga gccctgctca accactaata
    52021 agaatatgga caggggaatt gaatctttgg ttagataaag tcacagtctg ccaatacgga
    52081 aacgctccag cgttagacaa aaaaatcatt atattggtcc att
//
LOCUS       DAZGSP010000023        49380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZGSP010000023 DAZGSP010000000
VERSION     DAZGSP010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.023"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            45..569
                     /locus_tag="ACHK74_001502"
     CDS             45..569
                     /locus_tag="ACHK74_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HFP0220351.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            653..1339
                     /gene="lpfB"
                     /locus_tag="ACHK74_001503"
     CDS             653..1339
                     /gene="lpfB"
                     /locus_tag="ACHK74_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFP0220352.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            1363..3894
                     /gene="lpfC"
                     /locus_tag="ACHK74_001504"
     CDS             1363..3894
                     /gene="lpfC"
                     /locus_tag="ACHK74_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFP0220353.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYSALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            3905..4960
                     /gene="lpfD"
                     /locus_tag="ACHK74_001505"
     CDS             3905..4960
                     /gene="lpfD"
                     /locus_tag="ACHK74_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFP0220354.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            4966..5490
                     /gene="lpfE"
                     /locus_tag="ACHK74_001506"
     CDS             4966..5490
                     /gene="lpfE"
                     /locus_tag="ACHK74_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFP0220355.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5743..7434
                     /gene="eptB"
                     /locus_tag="ACHK74_001507"
     CDS             5743..7434
                     /gene="eptB"
                     /locus_tag="ACHK74_001507"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFP0220356.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            7526..7602
                     /locus_tag="ACHK74_001508"
     tRNA            7526..7602
                     /locus_tag="ACHK74_001508"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7560..7562,aa:Pro,seq:cgg)
     gene            7637..7770
                     /locus_tag="ACHK74_001509"
     ncRNA           7637..7770
                     /ncRNA_class="other"
                     /locus_tag="ACHK74_001509"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8515..10122
                     /gene="dppA"
                     /locus_tag="ACHK74_001510"
     CDS             8515..10122
                     /gene="dppA"
                     /locus_tag="ACHK74_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFP0220357.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVAA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10273..11292
                     /gene="dppB"
                     /locus_tag="ACHK74_001511"
     CDS             10273..11292
                     /gene="dppB"
                     /locus_tag="ACHK74_001511"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFP0220358.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11302..12204
                     /gene="dppC"
                     /locus_tag="ACHK74_001512"
     CDS             11302..12204
                     /gene="dppC"
                     /locus_tag="ACHK74_001512"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFP0220359.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12215..13198
                     /gene="dppD"
                     /locus_tag="ACHK74_001513"
     CDS             12215..13198
                     /gene="dppD"
                     /locus_tag="ACHK74_001513"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFP0220360.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13195..14199
                     /gene="dppF"
                     /locus_tag="ACHK74_001514"
     CDS             13195..14199
                     /gene="dppF"
                     /locus_tag="ACHK74_001514"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFP0220361.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14229..15500)
                     /locus_tag="ACHK74_001515"
     CDS             complement(14229..15500)
                     /locus_tag="ACHK74_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFP0220362.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            15976..16083
                     /locus_tag="ACHK74_001516"
     CDS             15976..16083
                     /locus_tag="ACHK74_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0220363.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16459..16566
                     /locus_tag="ACHK74_001517"
     CDS             16459..16566
                     /locus_tag="ACHK74_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0220364.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16931..17038
                     /locus_tag="ACHK74_001518"
     CDS             16931..17038
                     /locus_tag="ACHK74_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0220365.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(17125..18804)
                     /gene="bcsG"
                     /locus_tag="ACHK74_001519"
     CDS             complement(17125..18804)
                     /gene="bcsG"
                     /locus_tag="ACHK74_001519"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFP0220366.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(18801..18992)
                     /gene="bcsF"
                     /locus_tag="ACHK74_001520"
     CDS             complement(18801..18992)
                     /gene="bcsF"
                     /locus_tag="ACHK74_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFP0220367.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(18989..20494)
                     /gene="bcsE"
                     /locus_tag="ACHK74_001521"
     CDS             complement(18989..20494)
                     /gene="bcsE"
                     /locus_tag="ACHK74_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFP0220368.1"
                     /translation="MPAGGVWWFNVDRHEDAISLANQTIASQAETAHVAVISMDSDPA
                     KIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCSIDPHNYLFILVCANNAWQNIPA
                     ERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSLLLEEYRSLFGLASLRFQGDQHL
                     LDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAEIQPRSDEKRILSNVAVLEGAPP
                     LSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQIEPLARSIHTLRRQRGSAMKILV
                     RENTASLRATDEHLLLACGANMVIPWNAPLSRCLTMIESVQGQKFSRYVPEDITTLLS
                     MTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGVLVALRPVPGIRVEQALTLCRPN
                     RTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLPTGDIFSNRMVWFEDDQISAELV
                     QMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRIPEPMRLLDDAVERSS"
     gene            20833..21021
                     /gene="bcsR"
                     /locus_tag="ACHK74_001522"
     CDS             20833..21021
                     /gene="bcsR"
                     /locus_tag="ACHK74_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFP0220369.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            21033..21785
                     /gene="bcsQ"
                     /locus_tag="ACHK74_001523"
     CDS             21033..21785
                     /gene="bcsQ"
                     /locus_tag="ACHK74_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFP0220370.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            21782..24400
                     /gene="bcsA"
                     /locus_tag="ACHK74_001524"
     CDS             21782..24400
                     /gene="bcsA"
                     /locus_tag="ACHK74_001524"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFP0220371.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            24411..26749
                     /gene="bcsB"
                     /locus_tag="ACHK74_001525"
                     /pseudo
     CDS             24411..26749
                     /gene="bcsB"
                     /locus_tag="ACHK74_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
     gene            26756..27862
                     /gene="bcsZ"
                     /locus_tag="ACHK74_001526"
     CDS             26756..27862
                     /gene="bcsZ"
                     /locus_tag="ACHK74_001526"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFP0220372.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            27844..31317
                     /gene="bcsC"
                     /locus_tag="ACHK74_001527"
     CDS             27844..31317
                     /gene="bcsC"
                     /locus_tag="ACHK74_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFP0220373.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQHKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPANADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQRTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            31399..33387
                     /gene="hmsP"
                     /locus_tag="ACHK74_001528"
     CDS             31399..33387
                     /gene="hmsP"
                     /locus_tag="ACHK74_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFP0220374.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            33570..34856
                     /gene="dctA"
                     /locus_tag="ACHK74_001529"
     CDS             33570..34856
                     /gene="dctA"
                     /locus_tag="ACHK74_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFP0220375.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            35077..36576
                     /locus_tag="ACHK74_001530"
     CDS             35077..36576
                     /locus_tag="ACHK74_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP0220376.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(36672..37601)
                     /gene="kdgK"
                     /locus_tag="ACHK74_001531"
     CDS             complement(36672..37601)
                     /gene="kdgK"
                     /locus_tag="ACHK74_001531"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFP0220377.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            37833..38600
                     /gene="pdeH"
                     /locus_tag="ACHK74_001532"
     CDS             37833..38600
                     /gene="pdeH"
                     /locus_tag="ACHK74_001532"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFP0220378.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            38670..40730
                     /locus_tag="ACHK74_001533"
     CDS             38670..40730
                     /locus_tag="ACHK74_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP0220379.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKNANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(40964..42286)
                     /locus_tag="ACHK74_001534"
     CDS             complement(40964..42286)
                     /locus_tag="ACHK74_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0220380.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(42697..43710)
                     /gene="yhjD"
                     /locus_tag="ACHK74_001535"
     CDS             complement(42697..43710)
                     /gene="yhjD"
                     /locus_tag="ACHK74_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFP0220381.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(43759..44658)
                     /locus_tag="ACHK74_001536"
     CDS             complement(43759..44658)
                     /locus_tag="ACHK74_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0220382.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            45178..45779
                     /locus_tag="ACHK74_001537"
                     /pseudo
     CDS             45178..45779
                     /locus_tag="ACHK74_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
     gene            complement(45830..47479)
                     /locus_tag="ACHK74_001538"
     CDS             complement(45830..47479)
                     /locus_tag="ACHK74_001538"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFP0220383.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            47884..49281
                     /gene="ccp"
                     /locus_tag="ACHK74_001539"
     CDS             47884..49281
                     /gene="ccp"
                     /locus_tag="ACHK74_001539"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFP0220384.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT        12008 a        12271 c        13376 g        11725 t
ORIGIN      
        1 atgaacaaca tcaagcatat ttatctatat aaatggagtt ataaatgaaa aaggttctgt
       61 ttgccttatc tgcgcttgcg ctgacctcta cttccgtatt cgcagctgat gcgggcgacg
      121 gtactgttaa attcaccggt gagattgttg acgccgcttg tgttgtgtct ccagacactc
      181 aaaaacaaga agttgttctg ggtcaggtga acaagtctgt atttactgct atgggtgata
      241 aatccacagc aacgccgttc aaaatcaaac tggaaaactg cgatatctcc actttcaaaa
      301 atgtggaaat cagctttaac ggcgttggcg atgcagacaa cagcaaactg gtttctgtaa
      361 gcactgaacc aggtgccgca actggcgtag gtatcggtat ttatgataac accaataccc
      421 tggttgacct gaatactggt aaatctgcta ctgtcctgaa agaaggtcag accgttctgt
      481 atttcaccgc taactatgtc tctaccaaag atgcagtaac cacgggttac ggtaacgctg
      541 aagtcgactt caacctgact tacaactaat cgatcctgac ggaacgatca tctctcaatg
      601 gcaacagaga ctgtgttgcc attattcaca ccaatgactc agggaccgaa tcatgaaacg
      661 tattagcttt gctgtgctgg cacttttaat gaccgcacaa cagtgttttg ctggtggcgt
      721 agccttaagc acgacgcgtg ttatttatga tggcggcaag aaagaagcgt cgctgaccgt
      781 tgaaaaccat aataaaaatg aagagttttt gatccagtca tggattgatg atgtgaatgg
      841 caataaaaaa acaccgttta ttattacacc gccattattc aaacttgatc ctgataaaaa
      901 taacgtctta cgtatcgtca atattgaaga taccttacct aaagatcgtg aatccgttta
      961 ctggattaat gtaaaggcca ttcctgcgaa aagtgctgaa aatgaaaata aaaacgtctt
     1021 acagatagcc gttcgcaccc gtttgaagtt gttctatcgt ccaaaaggtt tgccgggaag
     1081 caacctcgag gccatgaaac agttacgttt ccagcgtcaa aataaccaaa ttgtggttaa
     1141 taacgactcc gcttataacc tgacctttaa tcagttttcc attaatggac aaaagattga
     1201 acgggcggga atggttctcg cgaaaggaaa gttaaacatt aaccttccag ccggtacagg
     1261 caacgcgcac gaagtgaaat acagcattat taatgatttc ggtgttgctg gcgaaatgct
     1321 gacgaagccg attaactgat ccttttacaa ggcgctttta ctatgcattg gatgcattta
     1381 cctctctatc actacagagc gcatttttcc ttctctctgc tggcattaac gattgccagc
     1441 tcgttacctg cgtatggcgg aaaatttaac cctaaatttc tcgaaaacgt tcagggtatc
     1501 gatcaacaca tcgacttgtc ggtatacgac tcacctgtcg gccagcaaat ccccggtaaa
     1561 taccgggttt cggtgtttgt aaacgaagaa aaaatggctt cccggacgct ggattttagt
     1621 accgccagcg aagcgaaacg caaagccagc ggtgagtcgc ttatgccttg ccttagccgg
     1681 gtgcaactgg aagaaatggg cgtgcgcgtt gacagctttc cggcgctgaa gatgtcacca
     1741 ccagaagcct gcgtagcgtt tgatgaaatc attccccagg ccaccagccg ctttgatttt
     1801 aatactcaga cgctgcatct ttcctttcca caggcggcaa tgatgatgac cgctcgcggc
     1861 acggttgatc cttctcgctg ggacgaagga atacctgcgc tgttgctgga ttacagcttt
     1921 agtggcagca atggacgcaa tgaaggtaca ggttcatctt cagattcgac cagtgacagt
     1981 tattacctga acctgcgcag cggtttgaac gttggtccgt ggcgtttacg taataacagt
     2041 atctggaacc gtaccgatgg caaaaaccaa tgggataacg ttggtacatc gttaaaccgg
     2101 gcaattattc cacttaaatc acagataact ctgggtgata ccgccacgcc tggcgagatt
     2161 tttgatagcg tacaaatgcg tggggcgtta ctggcatccg acgatgaaat gttgccggac
     2221 agccagcgtg gctttgctcc ggtagtgcgc ggcatcgcca aaagcaacgc tgaagtgagt
     2281 atcgaacaga acggctatgt catttatcgc acttttgttc agccgggtgc atttgagatt
     2341 aacgatctct acgcaacctc cggcagcggc gacctgacgg tgatcattaa agaagccgac
     2401 ggaagtgaac aacgttttat ccagccgttt tcggcggtgg cgattttcca gcgtgaaggg
     2461 tatctgaaat acagtctggc tgccggtgaa tatcgtgctg gtaattacga cagcgataaa
     2521 ccacatttcg ggcagtttac cgccatgtac ggcttgccgt ggggtatgac cgcctacggt
     2581 ggtgcattgt tgtcggcaga ttacagcgct ttagctctgg gcctggggaa aaacttcggt
     2641 accattggcg cagtatcggt ggatgtcact caggcgaaaa gtcagttacg caacaatgaa
     2701 aaagaggaag gccagtctta tcgcttcctc tattcgaaaa gttttgaggg tggcactgac
     2761 ctgcgtctgt tgggctacaa atactcaacc agcggctact acaccttcca ggaagcaact
     2821 gatgttcgca gtgacgccga cagtgattac cgccgttatc acaagcgcag ccagatacag
     2881 ggcaatatta cccagcaact gggagactac ggctcggttt atttcaatat gacgcagcag
     2941 gattactgga acgttgacgg taaggaaaac tccttaagcg ccggttatca cggtcacatt
     3001 ggtcgggtga attacagcgt ggcctatacc tggacgagaa gtccggaatg ggaagaagac
     3061 gatcgcttgt ggtcattcag tgtttccatc cctctggggg gcgcgtggag cagttatcgc
     3121 atgactaccg accagaatgg caagacgtcg caacaggcct ctgttagcgg cacgttactg
     3181 gaagatcgta acctgagcta taacgttcag cagggttaca ccagtaatgg cgtaggctac
     3241 agtggcagcg tcaacatggg gtatatgggt ggctctggta acattgacgt aggctataac
     3301 tattctaaag ataaccaaca ggttaactat ggcgtgcgcg gcggtgtcat cgttcacagt
     3361 gaaggtataa cgctttcgca gcctttaggc gaaagtctgg cgattgtttc tgcaccgggc
     3421 gcgcgtggtg gacatgtggt taacagcagc ggcgtagaag ttgactggat gggcaacgcc
     3481 gtggtgcctt atctcacacc atatcgtgaa accattgtgg aattgcgtag cgacacgttg
     3541 gggcaaaacg ttgaactaca ggaagcgttc cagaaagtcg tgcctactcg tggtgcagtt
     3601 gtgcgttccc gctttgatac ccgcgtcggt taccgtgtgc tgatgagctt aaaacaggcg
     3661 aatggcaacg cagtaccgtt tggtgcaacc gcagcgttaa ttgatgagag caaacctgcc
     3721 agcagcattg tgggtgaaga aggacagctt tatatcagcg ggatgccaga agagggcgaa
     3781 ttgcaggtga gctgggggaa cgagcaggcg caacgttgcc gtgtgccatt ccgcttgcca
     3841 gagaacaagg acaatgcggc tatcgtgatg gttaatgcgg tatgcgagaa ataaggatat
     3901 ttagatgaaa attaaaaatt tgttggcagg tatggtgctg ttagggacga gcacttttgc
     3961 tggaaatata tgggctgcgg attggggacc atgtcagaca tcaaatgggg tggcccatga
     4021 gtatagtttt gatttcgtac agactatcca ggtaccttca gaaaataagg ctgggaaaat
     4081 attaactcag ccctttgctt taggtacaaa atattccgct tattgtgaat gcccggatcc
     4141 cattccagat aatggagttg tgacttattt taaaggtgta acgcttctgc ctgagcctgg
     4201 aactgtagat gggtactata aattcaataa ttacattgat attctcacca aaatatatgt
     4261 ctacacacaa ttagatatcc cggtgccttt taccgatcga agcaatggca cggcacagaa
     4321 agaatgtacg ccctatactg ctaacaattg ggggacaggt ggtaaaggaa gtatcagtat
     4381 ttatatttca catccttttg ttgggcaaat gattattcct aaaacacgag ttgcgtccct
     4441 ctttgggaca aaaaagaaag gtgtttacaa tgatagtcag cccatggcca atgtcagtat
     4501 ttccgggagt attacagtta ctcaaggctg tgaacttgcc gctggcaccg tactggatat
     4561 tccattcgga gaatatcagg cccatgattt taaagggcgt gccggacaac caccacaaaa
     4621 tgttcagaag gtacaaaaag aattaagctt tgactgcaat aatatttcag atggcgtcaa
     4681 aatttatctc agtattgatg caacaccaaa tactgcctat cccagcgcaa ttgatttagg
     4741 caacgccgat gttggtgcag tcattgagga cggtaaaggt aatattctta agccgaatga
     4801 cagtaatagc ttattagaaa tgaacccagg ctcgctgtat gaagatgtca aacgcaaagc
     4861 gacaaccaca attaccgctt atcccgttag cactaccgga aaattacccg ctgccgggga
     4921 ttatagcggt attgctacca tacatgtcga actggagtaa cagtcatgaa gcgaacaata
     4981 cttgcgttat gcctgctgcc tctttccttt tcgtcattcg cggcaaccac ggatttaggg
     5041 ccgaaaggaa cattgaaatt cacccttaaa atcagccagg gtgcttgcga actggtgaaa
     5101 gacagcgttg aggttgacat gggcaccgcg gttctcaaaa agccggtaag aatcggaacg
     5161 gaaattaatc ccactccttt ttccatcggc ctgaaaaact gtagcgaagt ggtcagagcc
     5221 tacgtgacta tggacggtac acccaatgca gacgactcaa atctttttgc gctggatgcg
     5281 ggtggcgcta caggcgttgg gctgaaaatc aaaaccgctg ctggagtaca acagttcccg
     5341 gaaaatacaa cgccgacacc ggttgagttt gcaattaacg gtgatggcgc acatcaactg
     5401 aactatatcg ccagctacgt gcctattcgc gccgatgcaa ctattggtcg cgcagatgca
     5461 acggtcgact ttactgtaca gtacgaataa tttcacatct taagggccag ttaactggcc
     5521 ctttttactg ctttttgtaa cgctaaagtc tcttttcaaa cttgcatttt tgtaaatttg
     5581 tgcttcatgc acactctttc cccacacttt ttcgctttgc tgtggtctac ttattcgcgc
     5641 gtgtagattt tacttatctg actacctccg cacttttccc ctgccgggcc tgaaaagccg
     5701 ctaagcaggg tgttatcacc tgtttgtcca gggtttgttt gcatgagata catcaaatcg
     5761 attacacagc agaagctgag ctttttgctt gcaatctata ttggcctttt tatgaatggc
     5821 gcggtttttt atcgccgctt cggcagctat gcgcacgatt ttaccgtctg gaaaggcgtt
     5881 tctgctgttg ttgaactggc cgccaccgta ctggtgacct tctttttact acgtcttcta
     5941 tcgctgtttg gccgccgcag ctggcgtatt ctggcgtcgc tggtggtgct cttttccgca
     6001 ggtgccagct attacatgac cttccttaat gtggtcattg gttatggcat catcgcttcc
     6061 gtcatgacca ccgatatcga cctgtcaaaa gaagttgttg gtctgaactt tattctctgg
     6121 ttaatcgccg ttagtgcatt gcctcttatc cttatctgga ataaccgctg tcgctacacc
     6181 ttgctccgac aactgcgaac cccagggcag cgtattcgca gcctggcggt cgtcgtactg
     6241 gcgggtatta tggtttgggc accgattcgt ttgctggata tccagcagaa gaaagtggag
     6301 agggcgaccg gcgttgattt gccgagttat ggcggtgtcg tagcgaactc ttatctgcca
     6361 tcaaactggc tttctgcgtt ggggctgtat gcctgggcgc gggtcgatga atcttccgat
     6421 aataattcat tgcttaatcc ggcgaagaaa ttcacttatc aggcaccgca aaacgttgat
     6481 gacacttatg tcgtgtttat catcggtgaa accacgcgtt gggaccatat gggtattttc
     6541 ggctatgagc gtaataccac gccgaaactg gcccaggaga aaaatctggc ggcgttccgt
     6601 ggttactcct gtgataccgc aaccaaactc tcactgcgtt gcatgtttgt acgtcagggg
     6661 ggcgcggaag ataatccgca gcgcacatta aaagaacaga acattttcgc ggttctgaag
     6721 cagttaggat tcagttctga cctctacgct atgcagagcg aaatgtggtt ctacagcaac
     6781 acgatggcgg acaacattgc ttatcgtgag cagattggtg cggagccacg taatcgtggc
     6841 aagccggtag atgatatgtt gctggtagac gaaatgcagc aatcgctagg gcgcaacccg
     6901 gatggtaagc atctgatcat tctgcatacc aaaggttcgc attttaacta cacccagcgt
     6961 tatccgcgta gcttcgcgca gtggaagccg gaatgtattg gtgttgatag cggctgtacc
     7021 aaagcgcaga tgatcaactc ctatgacaac tcggtgacct atgtggatca ctttatctcc
     7081 agcgtgattg atcaggttcg cgataagaaa gcgattgtgt tctacgcagc tgaccacggt
     7141 gagtcaatta atgaacgcga gcacctgcac ggcacgccgc gtgaactggc accgccggag
     7201 cagttccgcg taccgatgat ggtctggatg tcagataaat atctggaaaa tccggccaat
     7261 gcgcaggcgt ttgcgcagct gaaaaaagaa gccgacatga aagtgccacg ccgtcacgta
     7321 gagctgtacg acaccatcat gggttgtctt ggctatactt caccggatgg tggaattaac
     7381 gaaaacaaca actggtgtca catcccgcag gcaaaagagg cagcggctaa ctaagcggcc
     7441 tgctgacttt ctcgccgatc aaaaggcatt ttgctattaa gggattgacg agggcgtatc
     7501 tgcgcagtaa gatgcgcccc gcattcggtg attggcgcag cctggtagcg cacttcgttc
     7561 gggacgaagg ggtcggaggt tcgaatcctc tatcaccgac caaattcgaa aagcctgctc
     7621 aacgagcagg cttttttgca tctgcagctc atgaggatga gaacctccgg gggcagggag
     7681 gttcgactcg agcgaagcga gagaacgttg cgccagcaac ggcccgcagg gcgagccacg
     7741 aagtggcgag taatcctcta tcaccgacca aattcgaaaa gcctgctcag cgagcaggct
     7801 tttttgcatc tgcagctcat gaggatgaga acctccgggg gcggggaggt tcgactcgac
     7861 gtaggcctga taagacgcgc cagcgtcgca tcaggcaacg gcaccgcact tattgtcgga
     7921 tgcggcgcga ccgccttatt cgaccaacac accacacatt ttcctgccaa ctttgcgccc
     7981 catatctcac tatccattaa cggatttgtg acagaaacca ttgtcttttt ttatatttgc
     8041 ttaaatcttt ttttgcgtta cttatggctg gcgctggcat tttccgctgt gcgttttagc
     8101 gttcatggta ttaatcgctc agatatccac cttttcctca gatttttttc attaaatgca
     8161 taaaacttaa tcaccaaatg ttgctaaata ttaagctgat tgttctggtc atgttggagt
     8221 agcacaaatt tctctgctgc aaatagctgt ttgaagtttt tttaatcttt gtttgtggat
     8281 tctcaccgtt ggtgtaaatc ccggttggct gtattgacgt tttcacattc tgttgacaga
     8341 ttgtaggtca cgaggggcat tttatggagg atccgcactg ttacactgat gttaattagt
     8401 acggcatccc cacctcataa cgttgacccg accgggcaaa aaacaaaaaa ggtcaggcag
     8461 cgacaaccca ctgcaaaggg ttaaaacaac aaacatcaca attggagcag aataatgcgt
     8521 atttccttga aaaagtcagg gatgctgaag cttggtctca gcctggtggc tatgaccgtc
     8581 gcagcaagtg ttcaggctaa aactctggtt tattgctcag aaggatctcc ggaagggttt
     8641 aacccgcagc tgtttacctc cggcaccacc tatgacgcct cttccgtacc gctttataac
     8701 cgcctggttg aatttaaaat cggcaccact gaagtgatcc cgggcctcgc tgaaaagtgg
     8761 gaagtcagcg aagacggtaa aacctatacc ttccatctgc gtaaaggtgt gaagtggcac
     8821 gacaataaag aattcaaacc gacgcgtgaa ctgaacgccg atgacgtggt gttctcgttt
     8881 gatcgtcaga aaaacgcgca aaacccgtac cataaagttt ctggcggcag ctacgaatac
     8941 ttcgaaggca tgggcttgcc ggagctgatc agcgaagtga aaaaggtgga cgacaacacc
     9001 gttcagtttg tgctgactcg cccggaagcg ccgttcctcg ctgatctggc aatggacttc
     9061 gcctctattc tgtcaaaaga atatgctgat gcgatgatga aagccggtac accggaaaaa
     9121 ctggacctca acccaatcgg caccggtccg ttccagttac agcagtacca aaaagattcc
     9181 cgtatccgct acaaagcgtt tgatggctac tggggcacca aaccgcagat cgatacgctg
     9241 gttttctcta ttacccctga tgcttccgtg cgttacgcga aattgcagaa gaatgaatgc
     9301 caggtgatgc cgtacccgaa cccggcagat atcgctcgca tgaagcagga taaatccatc
     9361 aacctgatgg aaatgccggg gctgaacgtc ggttatctct cgtataacgt gcagaaaaaa
     9421 ccactcgatg atgtgaaagt tcgtcaggct ctgacctacg cggtgaacaa agacgcgatc
     9481 atcaaagcgg tttatcaggg cgcgggcgtg gcagcgaaaa acctgatccc gccaaccatg
     9541 tggggctata acgacgacgt tcaggactac acctacgatc ctgaaaaagc gaaagccttg
     9601 ctgaaagaag cgggtctgga aaaaggtttc tccatcgacc tgtgggcgat gccggtacaa
     9661 cgtccgtata acccgaacgc tcgccgcatg gcggagatga ttcaggcaga ctgggcgaaa
     9721 gtcggcgtgc aggccaaaat tgtcacctac gaatggggtg agtacctcaa gcgtgcgaaa
     9781 gatggcgagc accagacggt aatgatgggc tggactggcg ataacgggga tccggataac
     9841 ttcttcgcca ctctgttcag ctgcgccgcc tctgaacaag gctccaacta ctcaaaatgg
     9901 tgctacaaac cgtttgaaga tctgattcaa ccggcgcgtg ctaccgacga ccataacaaa
     9961 cgcgttgaac tgtacaagca agcgcaggtg gtgatgcacg atcaggctcc ggcactgatc
    10021 atcgctcact ccaccgtgtt tgaaccggta cgcaaagaag ttaaaggcta tgtggttgat
    10081 ccattaggca aacatcactt cgaaaacgtc tctatcgaat aattaaaagc catacccggt
    10141 ggtgctgtgc ctctgcgtgc agcgccatcc ggcagcaata cttcattccc tgcccgacct
    10201 gggcgggaat tgatttgtga gcaatacaga cacgcagttc caggctgcgg gtcactatag
    10261 agaatccggg ttatgttgca gtttattctc cgacgtttgg gactcgtcat ccccacgttt
    10321 atcggtatta cccttctcac atttgccttt gtccacatga tcccgggcga tccggtgatg
    10381 atcatggcag gcgaacgtgg gatctcccct gagcgtcacg cgcagctgct ggctgaactc
    10441 ggcttagata aaccgatgtg gcagcagtat ctccattaca tttggggcgt aatgcacggc
    10501 gatctaggca tttcaatgaa aagccgaatt ccagtatggg aagagttcgt gccgcgcttt
    10561 caggccacgc tggaacttgg cgtctgcgcg atgatttttg ctacggcagt cggtattccg
    10621 gttggtgtgc tggctgcggt taaacgcggt tccattttcg atcacacagc ggttggcctg
    10681 gcgctgaccg gctactcgat gccgatcttc tggtggggca tgatgctgat catgctggtt
    10741 tcggtgcact ggaacctgac gcccgtctcc ggtcgcgtga gcgatatggt gttcctcgat
    10801 gactccaatc cgttaaccgg ttttatgcta atcgacaccg ccatctgggg tgaagacggc
    10861 aactttatcg atgccgtcgc ccatatgatc ttgcctgcca ttgtgctggg tactattccg
    10921 ctggcggtca ttgtgcgtat gacacgctcc tcgatgctgg aagtgctggg cgaggattac
    10981 atccgcaccg cgcgcgccaa agggctaacc cgcatgcggg tgattatcgt ccatgcgctg
    11041 cgtaacgcga tgctgccggt ggtgaccgtt atcggcctgc aggtgggaac attgctggcg
    11101 ggggcgattc tgaccgaaac catcttctcg tggcccggtc tgggacgctg gttgattgac
    11161 gcactgcaac gccgcgacta tccggtagtg cagggcggcg tattgctggt ggcgacgatg
    11221 attatcctcg tcaacttgct ggtcgatctg ctgtacggcg tggtgaaccc gcgtattcgt
    11281 cataagaagt aaggggacat catgtcacag gttactgaaa ataaagtgat tagcgcaccg
    11341 gtgccgatga ccccgttaca ggagttctgg cactatttta aacgcaacaa aggcgcggtc
    11401 gtcgggctgg tttacgtcgt catcgtgctg ttcatcgcga tctttgccaa ctggattgca
    11461 ccctataacc cggcggaaca gttccgcgat gcactgctcg ccccgccagc ctggcaggaa
    11521 ggcggcagca tggcgcactt gctgggcacc gatgacgtag gccgtgatgt gctgtcgcgc
    11581 ctgatgtacg gtgcgcgcct gtcgctgctg gttggctgtc tggtagttgt gttatcgctg
    11641 attatgggcg ttattctcgg cctgatcgcc ggttactttg gcggcctggt cgataacatc
    11701 attatgcgcg tggtcgatat catgctggcg ctgccaagtc tgctgctggc gctggtgctg
    11761 gtggcaattt tcggcccgtc gattggtaac gccgcgctgg cactgacctt cgttgccttg
    11821 ccgcactatg tgcgcttaac ccgcgccgcc gtgctggtgg aagttaaccg cgattacgtc
    11881 accgcgtctc gcgtggcggg tgccggggcg atgcgtcaga tgtttattaa catcttcccg
    11941 aactgccttg cgccgctgat tgttcaggcg tcgctcggtt tctctaacgc cattctcgat
    12001 atggctgctc ttggtttcct cggcatgggg gcacagccgc caacgcctga gtggggcacc
    12061 atgctctccg acgtgttgca gttcgcgcaa agcgcctggt gggtcgtgac cttcccgggt
    12121 ctggcgatcc tgctgacggt gctggcattt aacctgatgg gtgacggtct gcgtgacgcg
    12181 ctcgatccca aactgaagca gtaagaggtt cgagatggcg ttattaaatg tagataaatt
    12241 atcggtgcat ttcggcgacg aaagcgcgcc gttccgcgcc gtagaccgca tcagctacag
    12301 cgtaaaacag ggcgaagtgg tcgggattgt gggtgagtcc ggctccggta agtcggtcag
    12361 ttcactggcg attatggggc tgattgatta tccgggccgc gtaatggcag aaaaactgga
    12421 gtttaacggc caggatttgc agcgtatctc agaaaaagag cgccgcaacc tggtgggtgc
    12481 cgaagtggcg atgatcttcc aggacccgat gaccagcctt aacccgtgct acaccgtggg
    12541 tttccagatt atggaagcga ttaaggtgca tcagggcggc aacaaaagta cccgccgtca
    12601 gcgagcgatt gacctgctga atcaggtcgg tattcccgat ccggcatcgc gtctggatgt
    12661 ttacccgcat cagctttccg gcggcatgag ccagcgcgtg atgatcgcca tggcgattgc
    12721 ctgtcggcca aaactgctga ttgccgatga accgaccacc gcgctggacg tgaccattca
    12781 ggcgcaaatc atcgaactac tgctggagct acagcagaaa gagaacatgg cgctggtgtt
    12841 aattacccat gacctggcgc tggtggcgga agcggcacat aaaatcatcg tgatgtatgc
    12901 aggccaggtg gtggaaaccg gtgatgcgca cgccatcttc catgcgccgc gtcacccgta
    12961 tactcaggca ttgctgcgtg cgctgccaga atttgctcag gacaaagaac gtctggcgtc
    13021 gttgccaggt gtcgttcccg gcaagtacga ccgcccgaac ggctgcctgc ttaacccgcg
    13081 ctgcccctat gccactgaca gatgtcgcgc tgaagaaccg gcgctgaata tgctcgctga
    13141 cgggcgtcag tccaaatgcc attacccact tgatgatgcc gggaggccga cactatgagt
    13201 acgcaagagg ccaccctgca acaaccgctg ttgcaggcta tcgacctgaa aaaacattat
    13261 ccggtgaaga aaggcatgtt cgcgccggaa cgtctggtta aagcgctgga tggcgtttcg
    13321 tttaaccttg aacgtggcaa aacgctggca gtagtgggcg aatctggctg cggtaaatcg
    13381 accctcggtc ggttgctgac gatgattgaa atgcccaccg gtggcgagct gtattaccag
    13441 gggcaggatc tgcttaagca cgatccgcag gcgcagaagc tgcgtcggca gaaaatccag
    13501 atcgtcttcc agaaccctta cggttcgctg aatccgcgta aaaaagtcgg gcaaattctt
    13561 gaagagccgc tgctgatcaa caccagctta agcaaagaac agcgtcggga aaaagccctg
    13621 tcgatgatgg cgaaagtcgg cctgaaaacc gagcactatg accgctatcc gcatatgttc
    13681 tccggcggtc agcgtcagcg tatcgccatc gcccgtggtc tgatgctcga cccggatgtg
    13741 gtgattgccg atgaaccggt ttctgcgctg gatgtttcag tgcgcgcgca ggtgctgaat
    13801 ctgatgatgg atttgcagca ggagttgggg ctgtcttatg tctttatctc ccacgacctg
    13861 tcggtggtgg agcacattgc tgatgaagtg atggtgatgt acctgggccg ctgcgtggag
    13921 aagggaacga aagatcaaat cttcaataac ccgcgccatc cgtacactca ggcgctactt
    13981 tccgcaacgc cgcgcctgaa cccggacgat cgccgcgagc gcatcaagct cagcggtgaa
    14041 ctaccaagcc cactgaatcc accgccgggt tgcgccttca acgcccgctg tcgtcggcgc
    14101 ttcggcccct gcacccagtt gcagccgcag ctaaaagact acggcggtca actggtcgct
    14161 tgttttgctg ttgatcagga tgaaaatccg cagcgttaac tcacaaaacc ggggccatgc
    14221 gctccggttt actcaatcag cttcaacagt ggcgacaagc atagcaaaat gccgtaaagc
    14281 agaattagcc aggctttaaa tccgcgaagt ttttccagtt gtgcgacttt atagatcagg
    14341 aaaaacggaa tgaggcacga cacaatgcca tataacgggc ttcccaactg aaagaacacc
    14401 agcaccgata cacgaaacga aacccaaatc gtcaatgtaa tgacgataaa agcgcagatc
    14461 gccagagtca gcatgcgtga gttaattttc ttggtatcaa taattcggct taacagattg
    14521 agaataatgc ctttaatagc ctcgtggaaa ccgagataaa tgccaaagaa tgcggtcagt
    14581 acggcaaaga tattcagcac cgtagaggtg atatgaatga tatgtccagg gatcacctgc
    14641 gcggccagcg ccagtgccga gatattttgt tcaaaggcag aaacggcttc ttcgtggcta
    14701 attgagaagg taaacgaaaa ggcaaaaaac aggatcaccg cgatgagcgt gatataacta
    14761 attcggtggg tacgcagcgc gagccgggtt gccagtactt tatctgcttc ccgtttacga
    14821 taggcaatat tcattgggtt aagtacctga ataaataccg cagaaaagaa gcaaaatgga
    14881 atggtaagca ggacatcgcg gaaaaagacg gaggcttgcg ggaaggcggt tatattggcg
    14941 aaattccagt gcgggatcat cgcaaaaccg aacacgacaa taatccctac tttgaccacc
    15001 accattggcc cggaaatctt aaacagtaat cgttcaccac cagatgcaat cgccaccagt
    15061 acggcgaaaa tagcgacttt ataaagtaga gattgtgaaa gatcggcatc ggttaaaccg
    15121 aaggttttca ggtacgaggc gctgtcgaaa accacggaga gagagtagat aaaaatcccg
    15181 tggataatca tcaaaaagta gataaccccg aggaaaattc cccagttctt ccccaggtaa
    15241 tgactgataa tatcggtgta gtcattacag gaatcgcttt cagaaagggt ttttaaataa
    15301 atgtcctgca ctacccaggt ggcaggataa gcaatgatcg ctgcggtaat aaatacccaa
    15361 attcccttca agccaatttg tactggcatc agcacggttc cggcaccaat cgccatgcct
    15421 atgcataata aaacccagcc gaagtcgtag cgtgtaaacg gcaatttctg attgtgtttc
    15481 gatagtgtgt tgtgctgcat aaagtcctgt ccagaaagaa gtcggcctcg caaaaatgaa
    15541 taaacccagt ttctcttgcg caacaggtgg agaagtcgaa tacgcagtat gtaaaaaatg
    15601 aggcaacaga ttcactgggg aggtcgcgag gaggcgcaat tctacaaaat tgcgcatttc
    15661 actaccagtc tggagtcagc ccggatatga actgcaacta ttgcaaataa agaaattaag
    15721 ctggaaggca tcgcgcaatg aggaagattg ccgggtgctg acagtaaaaa gcaatcgtaa
    15781 aatcagcggc aagccggaag ggttccggtg aggcgcaatg ttgcgggggc tttatccctg
    15841 gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg caggtatata cctgacaaca
    15901 taacgggggc taatcttgaa cctagaccac tcaagaatag ccgcgaaacg ttgtcattac
    15961 aacacaggcg gctatatgac gctcgcagag ctgggcatgg ccttctggca tgatttagcg
    16021 gctccggtca ttgctggcat tcttgccagt atgatcgtga actggctgaa caagcggaag
    16081 tagcgtgtca tgtgggcgtc aggctgccgt aatggcaatt tgcgcccgga ccaggccgca
    16141 ggagtgaaac tctgcggcct ttttcgtaag aaaaatgcaa cggttgcaag cggataatat
    16201 tctgcggaat gcgcagcaca gagtgaagat gagacggatt ccggaacaga aaaacagtcg
    16261 tagaatcagc gtcaagccgg aaggattccg gtgaggcgca atgttgcggg ggcttaatcc
    16321 ctggtggcat tggttgctgg agagagaaaa cccccgcacg ttgcaggtat gcacctgaca
    16381 acaccacggg ggccattctt gcatccaaac aatacaagaa tagccgcgaa acgttgtcat
    16441 tacaatacag gcggctatat gacgctcgca gaactgggca tggccttctg gcatgattta
    16501 gcggctccgg tcattgctgg cattcttgcc agtatgatcg tgaactggct gaacaagcgg
    16561 aagtaacgtg tcatgcgggc gtcaggctgc cgtaatggca atttgcgccc ggaccaggcc
    16621 gcaggggtga aactctgcgg cctttttcgt aagaaaaatg caacggttgc aagcggataa
    16681 tattctgcgg aatgcgcagc acagagtgag taagtgatgg atttcggaac agaaaaacag
    16741 tcgtagaatc agcggcaagc cggaaaggcg caatgttgcg ggggctttat ccctggtggc
    16801 attggttgct ggagagagaa aacccccgca cgttgcaggt atgcacctga caacataacg
    16861 ggggctaatc ttgaacccaa gcaactcaag aatagccgcg aaacgttgtc attacaacac
    16921 aggcggctat atgacgctcg cagagctggg catggccttc tggcatgatt tagcggctcc
    16981 ggtcattgct ggcattcttg ccagtatgat cgtgaactgg ctgaataagc ggaagtaacg
    17041 tgtcatgcgg gcgtcaggct gccgtaatgg caatttgcgc ccggatcagg ccgcagaggt
    17101 taaactctgc ggcctttttc gtgcttactg cgggtaaggc acccagtcgc cgccgttcag
    17161 gcgaacgtac ggtttatcct ggtattgaat aactactgca tttgagttct cggagaccgg
    17221 tgctgtttgt ggcaacccac tggtgagttt tttccagtca acattgtctt cggtgaaaat
    17281 cttgccatca agaacgcgaa ccaccagatc ggagatagcc aggaagctgc tcggttggtc
    17341 aatgacaatc ggtgccccct gatgtggtgc cttcatgccg aagaatttca ccccaacggg
    17401 gacgtctgtg atagacgggc tagggatatc acgtaggcca gatacctgca ttctgtcgcc
    17461 cttcagcgcg ccgccgtgtt ccggcaccac gaccaccatc actttacgac ccgatttctc
    17521 cagttcagta aagaaggcgt ccagttcatc aaagaatttc tgcgcccgcg ctttgtaatc
    17581 cgctgttttg ctgacccccg gataatggtt gccgtcatgc agtggaagcg tgttgtagaa
    17641 cgtggcacta cggctatttt tatctttttc ggtaacgtcc agccagcggt taagcacggc
    17701 ggtatcgtca taaaccggcg aaccatcaaa gcccagcaaa ataaccggca gatttgtttg
    17761 atccatcaat tcagtctgca tgccgccatt ttcgcgaact tctttcaaaa aaccgccgaa
    17821 ctggccgtta tgccccatca tcaggtgctg ggtaaagccc agtttcgaaa ggttatcaaa
    17881 cagatagcag tcgttatttg ccggttgata cagattagtg tgcgaagtct gcccgcagct
    17941 ggcgcgcagt aaacggatcg ccgccgggcc actgtaggag gtggcggagt taaagttctt
    18001 gaactcaata tcgaaatgcg accacagtgg atgcgacatc aacccggcgg cttctatatc
    18061 cgaccaggaa agcgaacaga tgttaatcac cagtagttca aatggctgag catcagcggg
    18121 cagcgaagac gggaaggtcg atttacgttt cgcctccgcg ttatagaaat tattcagcca
    18181 ggcgttaagg ttcgccgttg ttggcggtgc agtttgtgcg ggcatatcac ccactaccgg
    18241 tgcgccaccc gtcgccgcaa cggttgccgc tgcgttacca cccgtcgttg ttacagtggt
    18301 cgtcggttgt ccggctggcc acaaggagaa acttggtccc gccagggtaa gtacgttcag
    18361 ccatagcagt atggcaacca caaaaacggt aatgcgaatc cattgtgaca ggaataacca
    18421 ggccactaat aaaacaaaaa tggccccaat catctgccag ttaataaagc gtgtgacaag
    18481 gtcgattaaa taatcggtac tgaaccccgc cacctgcgaa ccctggctca ttatgctttc
    18541 cgggccaggc aaccaggtgt catgccagaa caaagcaaag ccgatcggca gggcaatcca
    18601 gtggcgcaag cgatgcaggc tgtagcgcgg aatgggcatc agcagaaacg cggcaaacac
    18661 caaattgagg agcggatgga agttaagata tcccgcccac aacaggccga acttaaccag
    18721 aaaatagaag ttccagccgg aaaggccgcg ccagtattgc cagagggaag aaggcatggc
    18781 ggtattttgc gtaaattgag tcattttttg gttgccctgg ctttttccgt gcggcgtaac
    18841 gtcccggccg gtttaacata acgaggttta gcaaagaaca aacgtaaggt gtcgcgaatg
    18901 cgtcgcaaag agtgccgcgc cagatagccc agcgggaaaa atatcagtgc gcaaacgaca
    18961 ataatttcaa tgatatcgct gatggtcatc atgatgagcg ctccacagca tcatctaaca
    19021 gtcgcatggg ttctggtatt cgtcgccagt ggcgaccatc gtgctcggca ttgatgaccg
    19081 gtttagaact ttgcgttaaa ggcagcggca tgccccattg ttctggggca agcaagcgca
    19141 tctgcaccag ctcggcactg atttgatcat cttcaaacca gaccatacgg tttgagaaaa
    19201 tgtcgccagt aggcaatggg aaaatatgat tcaacgcggt atccagatcg ttaatccgac
    19261 agaatgagag aaacagcacc agccgattac cgccaatggt catgatatcg ccggtacggt
    19321 tagggcgaca cagcgtcagg gcttgttcaa cgcggatacc cggtaccgga cgtagggcaa
    19381 ccagaacgcc tttaccgtgg gcaggtaata gagggttatt catcatgttg ttgacggcat
    19441 tacagaacac atcccacttc tggaaaccac gcagtttgag cggctgggtc attgacagca
    19501 aggtagtgat atcttccggc acatagcgac taaacttctg cccttgcacg ctttcgatca
    19561 tcgtcagaca acgggagagt ggcgcattcc acggaataac catatttgca ccgcaggcca
    19621 ataacaaatg ttcatcggtg gcgcgcaggc tagcggtatt ttcccgcacg aggattttca
    19681 tcgcactacc gcgctggcga cgcagggtat gaatgctgcg ggccagtggc tcgatttgcg
    19741 cattttgctg taaagaaaag accaccgtcg ccgcctgagc ggtacgggct tcattgaaca
    19801 ggacttcgtt attgttgaac agttgccagt gttccgatag cggcggcgca ccttccagta
    19861 cagcaacatt actcagaatg cgtttttcgt cgctgcgtgg ttggatctcc gcctcttcgc
    19921 tttgaactaa tgtccagata ccattttgtt gctgaacgct aagctgctga cgggcgctga
    19981 cccctttttc gttgcaccag aaggcaatat ccagcaaatg ttggtcaccc tgaaaacgca
    20041 aactggcaag accaaaaagt gaacggtatt cctcaagcaa caatgaaaat tgtttatcgt
    20101 tattatttcc gggattaatt accaaaagcg aacaatggtt taacctgctc catttattca
    20161 ttttatccaa ccatgagcga agccgctcgg caggaatgtt ttgccatgcg ttatttgcgc
    20221 aaacaagaat aaaaaggtaa ttatggggat caatagaaca ctgcaaatca cgggtcaaat
    20281 agtatagacc tttttcatga tttagcattg aaaataattt tattttttcc ggcccttgag
    20341 aatcatctaa ttgaaagatt ttcgccggat cgctgtccat gctaatgacc gcgacgtgtg
    20401 cggtttcagc ctgggatgca attgtttgat tcgccagact gatagcatct tcatggcgat
    20461 cgacgttaaa ccaccagacg ccgcctgctg gcatatgtca cagctcatcc cataatgatg
    20521 agataccgat agagaataca gggtccacaa tgtccctcat gtcaccattt tgtctgatta
    20581 tcagtttact agcgaaagct aagaaataaa cctaccattg aaattaaaac ttatcagatt
    20641 tagcatgtga atcaatttag aaggtcacaa gacgtgtgat tttagtcttt tcgtttttaa
    20701 ctttttctgt tgcgtaacgg aaagtcaaaa agtgagcaaa ttcccgtctc gccgtaaaca
    20761 ataaaccggc gaaatgctca cggttagaat tgtctcatga acggtacggt tatttcatag
    20821 ggatcaagca aaatgaataa caatgaacca gatactctgc ctgatcccgc gataggctat
    20881 atcttccaga atgatattgt ggcgttaaag caggcatttt cactgcctga tattgattat
    20941 gccgatattt cccaacgcga acagttggcc gcggcattaa aacgctggcc gttgctggca
    21001 gagtttgcgc aacaaaagta ggggattggt gaatggccgt actgggattg cagggggtgc
    21061 ggggaggcgt ggggacaaca accatcaccg ccgcattagc ctggtcatta caaatgttgg
    21121 gagaaaatgt tctggtggtc gatgcctgcc cggacaactt gttgcgcctg tcatttaatg
    21181 ttgattttac ccaccgtcag ggctgggcca gagcgatgct ggatggccag gactggcgtg
    21241 acgctgggtt gcgctacacc tcgcagctcg atttgctgcc ttttggtcag ttatccattg
    21301 aagaacaaga aaatccacag cactggcaaa cccggctgag cgatatttgc tccggcttac
    21361 agcaactaaa agccagcggg cgttaccagt ggattttaat cgacttaccg cgtgatgcct
    21421 cgcagataac ccaccagctg ctgagtttgt gcgatcactc gctggcaatc gtcaatgtgg
    21481 atgccaactg ccatatccga ctgcatcagc aagcgctgcc ggatggcgca catattttga
    21541 ttaatgactt ccgtattggc agtcaggttc aggacgatat ttaccagctt tggttgcaaa
    21601 gccagcgccg attactgccg atgctcattc atcgtgatga agcgatggct gaatgcctgg
    21661 cggctaagca accagtaggt gaatatcgca gtgatgcgct ggcggctgaa gagatactga
    21721 cgctggcgaa ctggtgcctg ttgaactact ccgggctgaa aacgccagtc gggagtgcat
    21781 catgagtatc ctgacccggt ggttgcttat cccgccggtc aacgcgcggc ttatcgggcg
    21841 ttatcgcgat tatcgtcgtc acggtgcgtc ggctttcagc gcgacgctcg gctgtttctg
    21901 gatgatcctg gcctggattt ttattccgct ggagcacccg cgctggcagc gtattcgcgc
    21961 agaacataaa aacctgtatc cgcatatcaa cgcctcgcgt ccgcgtccgc tggacccggt
    22021 ccgttatctc attcaaacat gctggttatt gatcggtgca tcgcgcaaag aaacgccgaa
    22081 accgcgcagg cgggcatttt caggtctgca aaatattcgt ggacgttacc atcaatggat
    22141 gaacgagctg cctgagcgcg ttagccataa aacacagcat ctggatgaga aaaaagagct
    22201 cggtcatttg agtgccgggg cgcggcggtt gatcctcggt atcatcgtca ccttctcgct
    22261 gattctggcg ttaatctgcg ttactcagcc gtttaacccg ctggcgcagt ttatcttcct
    22321 gatgctgctg tggggggtag cgctgatcgt acggcggatg ccggggcgct tctcggcgct
    22381 aatgttgatt gtgctgtcgc tgaccgtttc ttgccgttat atctggtggc gttacacctc
    22441 tacgctgaac tgggacgatc cggtcagcct ggtgtgcggg cttattctgc tcttcgctga
    22501 aacgtacgcg tggattgtgc tggtgctcgg ctacttccag gtagtatggc cgctgaatcg
    22561 tcagccggtg ccattgccga aagatatgtc gctgtggccg tcggtggata tctttgtccc
    22621 gacttacaac gaagatctca acgtggtgaa aaataccatt tacgcctcgc tgggtatcga
    22681 ctggccgaaa gacaaactga acatctggat cctcgatgac ggcggcaggg aagagtttcg
    22741 ccagtttgcg caaaacgtgg gggtgaaata tatcgcccgt accactcatg aacatgcgaa
    22801 agcgggcaac atcaacaatg cgctgaaata tgccaaaggc gagttcgtgt cgattttcga
    22861 ctgcgaccac gtaccaacgc gatcgttcct gcaaatgacc atgggctggt tcctgaaaga
    22921 gaaacagctg gcgatgatgc agactccaca ccacttcttc tcgccggacc cgtttgaacg
    22981 caacctgggg cgttttcgta aaacaccgaa cgaaggcacg ctgttctatg gtctggtgca
    23041 ggatggcaac gatatgtggg acgccacttt cttctgcggt tcctgtgcag taattcgtcg
    23101 caaaccattg gatgaaattg gcggtattgc tgtcgaaacg gtaaccgaag atgcgcacac
    23161 gtctctgcgc ctgcaccgtc gtggctacac ctctgcatat atgcgtattc cgcaggcggc
    23221 ggggctggcg accgaaagtc tgtcggcgca tatcggtcag cgtattcgct gggcacgcgg
    23281 gatggtgcaa atcttccgtc tcgataaccc gcttaccggt aaagggctga aattcgccca
    23341 gcggctgtgc tacgtcaacg ccatgttcca cttcttgtcg ggcattccac gactgatctt
    23401 cctgactgcg ccgctggcgt tcctgctgct tcatgcctac atcatctatg cgccagcgtt
    23461 gatgatcgcc ctgttcgtgc tgccgcatat gatccatgcc agcctgacca actcgaagat
    23521 ccagggcaaa tatcgccact ctttctggag tgaaatctac gaaacggtgc tggcgtggta
    23581 tatcgcacca ccgacgctgg tggcgctgat taacccgcac aaaggcaaat ttaacgtcac
    23641 cgccaaaggt ggactggtgg aagaagagta cgtcgactgg gtgatctcgc ggccctacat
    23701 cttccttgtt ctactcaacc tggtgggcgt tgcggtaggc atctggcgct acttctatgg
    23761 cccgccaacc gagatgctca ccgtggtcgt cagtatggtg tgggtgttct acaacctgat
    23821 tgttcttggc ggcgcagttg cggtatcggt agaaagcaaa caggtacgcc gatcgcaccg
    23881 cgtggagatg acgatgcccg cggcaattgc ccgcgaagat ggtcacctct tctcgtgtac
    23941 cgttcaggat ttctccgacg gtggtttggg gatcaagatc aacggtcagg cgcagattct
    24001 ggaagggcag aaagtgaatc tgttgcttaa acgcggtcag caggaatacg tcttcccgac
    24061 ccaggtggcg cgcgtgatgg gtaatgaagt tgggctgaaa ttaatgccgc tcaccaccca
    24121 gcaacatatc gattttgtgc agtgtacgtt tgcccgtgcg gatacatggg cgctctggca
    24181 ggacagctac ccggaagata agccgctgga aagtctgctg gatattctga agctcggctt
    24241 ccgtggctac cgccatctgg cggagtttgc gccttcttcg gtgaagggca tattccgtgt
    24301 gctgacttct ctggtttcct gggttgtatc gtttattccg cgccgcccgg agcggagcga
    24361 aacggcacaa ccatcggatc aggctttggc tcaacaatga tgataacgcg atgaaaagaa
    24421 aactattctg gatttgtgca gtggctatgg ggatgagtgc gttcccctct ttcatgacgc
    24481 aggcgacgcc agcaacgcaa ccactgatca atgctgagcc agctgtagcc gcccagacgg
    24541 aacaaaatcc gcaggtgggg caagtgatgc cgggcgtgca gggcgctgat gcgccagtcg
    24601 tggcgcagaa cggtccttcg cgtgatgtga agctgacctt tgcgcaaatt gcgccgccgc
    24661 cgggcagcat ggtgctacgt ggcattaacc cgaacggcag cattgagttt ggtatgcgca
    24721 gcgatgaagt ggtgacgaag gcgatgctca acctcgaata cacgccatcg ccatcgttac
    24781 tgcctgtcca gtcgcagtta aaggtttatc tcaatgatga gctgatgggc gtgctgccag
    24841 tgaccaaaga acagttgggt aaaaaacgct ggcgcaaatg cccattaacc cactgtttat
    24901 taccgacttc aaccgtgtgc ggctggagtt tgtcggccat tatcaggacg tgtgcgaaaa
    24961 cccggccagc accacgcttt ggctggatgt tgggcggagc agtggactgg atctgaccta
    25021 tcagaccctg aatgtgaaga atgacctgtc acacttcccg gtgccattct ttgacccgcg
    25081 cgataaccgt actaacacct tgccgatggt ctttgcgggt gcgccggatg ttgggctgca
    25141 acaagcctct gccattgtcg cctcgtggtt tggttcgcgt tctggctggc gtgggcagaa
    25201 cttcccggtg ctctataacc aactgccgga tcgtaatgcc attgtctttg caaccaacga
    25261 caaacggccg gacttcctgc gcgatcatcc ggcggtaaaa gcccctgtga ttgagatgat
    25321 taaccatccg cagaatcctt acgtcaaact gctggtggtg tttggtcgtg acgacaaaga
    25381 cctgttgcag gcagcgaaag gtatcgctca gggtaacatt ctgttccgtg gtgaaagcgt
    25441 ggtagtgaat gaagtgaaac cgctgctacc gcgtaagccg tacgatgcgc cgaactgggt
    25501 acgtaccgat cgtccggtca catttggcga actgaaaacc tatgaagaac agttacaatc
    25561 cagcggtctt gagccagcag cgattaacgt ttcgctaaac ctgccgccgg atctctacct
    25621 gatgcgcagt accggcattg atatggatat taattaccgc tacaccatgc cgccggtgaa
    25681 agacagttcg cggatggata tcagcctgaa taaccagttc ctgcaatcct tcaacctgag
    25741 cagcaaacag gaggcgaacc gcctgctgct gcggattccg gtattacaag gtttgctgga
    25801 tggcaaaaca gatgtctcta ttccggcgct gaaactgggc gcgaccaacc agctgcgctt
    25861 cgactttgag tatatgaacc cgatgccggg cggttcggtg gataactgta ttaccttcca
    25921 gccggtgcag aatcatgtgg tgattggtga cgactccacc atcgacttct cgaagtatta
    25981 ccacttcatc ccgatgccgg atctacgcgc ctttgctaac gcgggcttcc cattcagccg
    26041 gatggcggat ctgtcgcaaa ccatcaccgt gatgccgaaa gcgcctaacg aagcacagat
    26101 ggaaacgttg ctgaatactg ttggttttat cggcgcacag acgggcttcc cggcgattaa
    26161 tctgacggtg accgatgatg gcagcaccat tcagggcaaa gatgccgaca tcatgatcat
    26221 cggtggtatc ccggacaaac tgaaagacga taagcagatc gacctattgg tgcaggcgac
    26281 cgaaagctgg gtgaaaacac cgatgcgcca gaccccgttc cccggcattg tgccggacga
    26341 gagcgatcgc gcggcagaaa cccggtcaac gctgacctct tccggtgcga tggcggcggt
    26401 gattggcttc cagtcgccgt ataacgacca gcgcagcgtg attgcgctgt tggcagatag
    26461 cccacgcggt tatgaaatgc ttaacgatgc ggtgaacgat agcggcaaac gcgccaccat
    26521 gttcggttcg gtcgcggtga tccgcgagtc cggtatcaac agcctacgtg ttggcgacgt
    26581 ttattacgta ggtcatctgc cgtggttcga gcgcgtgtgg tatgcgctgg caaaccatcc
    26641 gattctgctg gcggtgctgg cggctatcag tgtgatattg ctggcatggg tactgtggcg
    26701 tctgctgcga attattagtc gtcgtcgtct taacccggat aacgagtaat tgaagatgaa
    26761 tgtgttgcgt agtggaatcg tgacgatgct gctgttggct gcctttagtg ttcaggcagc
    26821 ctgtacctgg cctgcctggg agcagtttaa aaaggattac atcagtcagg aagggcgcgt
    26881 catcgacccc agcgacgcgc gcaaaatcac cacctccgaa gggcaaagtt acggtatgtt
    26941 ctttgccctg gcggctaacg accgtgtagc tttcgataat attctcgact ggacgcagaa
    27001 caatctcgct cagggttctt taaaagaacg tttgcccgcc tggctgtggg gcaagaaaga
    27061 gaacagtaag tgggaagtgc tggacagcaa ttcggcctcc gatggtgatg tctggatggc
    27121 ctggtcgttg ctggaggcgg ggcgtttgtg gaaagagcag cgttataccg acatcggcag
    27181 cgcgttgcta aaacgtatcg cgcgggagga agtggtgacg gtgcctgggc tgggttccat
    27241 gttgttaccg ggcaaagtgg gttttgctga ggataacagc tggcgtttta accccagcta
    27301 cctgccgccg acgctggcgc agtatttcac ccgctttggc gcgccgtgga ccacgctgcg
    27361 cgaaaccaat caacgtttat tgctggaaac cgccccgaaa ggtttttcgc cagactgggt
    27421 gcgctatgag aaagacaaag gctggcagct aaaagccgaa aaaacattga tcagcagcta
    27481 cgacgctatc cgcgtttaca tgtgggtagg catgatgcct gacagcgatc cgcaaaaagc
    27541 gcggatgctc aaccggttta aaccgatggc gacattcact gagaaaaacg gttatccgcc
    27601 ggaaaaagtg gatgtggcta cggggaaagc gcagggtaaa ggaccggtcg gtttttctgc
    27661 cgccatgctg ccctttttac aaaaccgcga tgcgcaggcc gttcagcgcc agcgcgtggc
    27721 cgataacttt cccggcagcg atgcctatta caactatgtg ctgaccctgt ttggacaagg
    27781 ctgggatcaa caccgtttcc gcttctcgac aaaaggtgag ttattacctg actggggcca
    27841 ggaatgcgca aattcacact aaacatattc acgctttccc tcggtctggc cgtcatgccg
    27901 atggtcgagg cagcaccaac cgctcagcaa cagttgctgg agcaagttcg gttaggcgaa
    27961 gcgacccatc gtgaagatct ggtgcaacag tcgttatatc ggctggaact tattgatccg
    28021 aataacccgg acgtcgttgc cgcccgtttc cgttctttgt tacgtcaggg cgatattgat
    28081 ggcgcgcaaa aacagctcga tcggctgtcg cagttagcgc cgagttcaaa tgcgtataaa
    28141 tcgtcgcgga ctacgatgct actttccacg ccggatggtc gtcaggcact gcaacaggca
    28201 cgattgcagg cgacgaccgg tcatgcagaa gaagctgtgg cgagttacaa caaactgttc
    28261 aacggtgcgc cgccggaagg tgacattgct gtcgagtact ggagtacggt ggcgaaaatt
    28321 ccggctcgcc gtggcgaagc gattaatcag ttaaaacgca tcaatgcgga tgcaccgggc
    28381 aatacgggcc tgcaaaacaa tctggcgcta ttgctgttta gtagcgatcg ccgtgacgaa
    28441 ggttttgccg tcctggaaca gatggcaaaa tcgaacgccg ggcgcgaagg ggcctctaaa
    28501 atctggtacg ggcagattaa agacatgccc gtcagtgatg ccagtgtgtc ggcgctgaaa
    28561 aaatatctct cgatctttag tgatggcgat agcgtggcgg ctgcgcaatc gcaactggca
    28621 gaacagcaaa aacagctggc cgatcctgct ttccgcgctc gtgcgcaagg tttagcggcg
    28681 gtggactctg gtatggcggg taaagccatt cccgaactac aacaggcggt gcgggcgaac
    28741 ccgaaagaca gtgaagctct gggggcgctg ggccaggcgt attctcagaa aggcgatcgc
    28801 gccaatgcag tggcgaatct ggaaaaagcc ctcgcactgg acccgcacag cagcaacaac
    28861 gacaaatgga acagtctgct gaaagtaaac cgctactggc tggcgatcca gcagggcgat
    28921 gctgcgctga aagccaataa tcctgaccgg gcagaacgcc tgttccagca ggcgcgtaat
    28981 gtcgataaca ccgacagtta tgcagtgctg gggctgggcg atgtggcgat ggcgcgaaaa
    29041 gattatcccg ccgccgaacg ttattatcag cagaccttgc gtatggacag cggcaacact
    29101 aacgccgtgc gcgggctggc aaatatttac cgccagcaat cgccagaaaa agctgaagcg
    29161 tttatcgcct cgctctctgc cagtcagcgg cgtagcattg atgatatcga acgcagcctg
    29221 caaaacgacc gtctggcaca gcaggcagag gcactggaaa accagggcaa atgggcgcag
    29281 gcggcagcac ttcagcggca acgactggcg ctggaccccg gcagcgtatg gattacttac
    29341 cgactttcgc aggatctctg gcaggccgga caacgcagcc aggccgatac gttaatgcgc
    29401 aatctggcgc agcataagcc gaacgacccg gagcaggttt acgcttacgg gctgtacctc
    29461 tctggtcatg accaggacag agcggcgctg gcgcatatca acagcctgcc gcgcgcgcag
    29521 tggaacagca atattcagga gctggttaat cgactgcaaa gcgatcaggt gctggaaacc
    29581 gctaatcgcc tgcgagaaag cggcaaagaa gctgaagcgg aagcgatgct gcgtcagcaa
    29641 ccaccttcca cgcgtattga cctcacgctg gctgactggg cgcaacaacg acgtgattac
    29701 accgccgccc gtgctgcata tcagaatgtc ctgacgcggg agccagctaa cgccgacgcc
    29761 attcttggtc tgacggaagt ggatattgct gccggtgaca aagcggcggc acgtagccag
    29821 ctggcgaaac tgcccgctac cgataacgcc tcgctgaaca cacagcggcg cgtggcgctg
    29881 gcacaggcgc agcttggcga taccgcagca gcgcagcgga cgtttaataa gttgatcccg
    29941 caggcaaaat ctcagccacc gtcgatggaa agcgcgatgg tgctgcgcga cggtgcgaag
    30001 tttgaagcgc aggcgggcga tccaacgcag gcgctggaaa cctacaaaga cgccatggtc
    30061 gcatccggtg tgaccacgac gcgtccgcag gataacgaca cctttacccg actgacccgt
    30121 aacgacgaga aagatgactg gctgaaacgc ggcgtgcgca gcgatgcggc ggacctctat
    30181 cgccagcagg atcttaacgt caccctcgag cacgattact ggggttcgag cggcaccggt
    30241 ggttactccg atctgaaagc gcacactacc atgttgcagg tggatgcgcc gtattctgac
    30301 gggcggatgt tctttcgcag tgatttcgtc aatatgaacg tcggcagttt ctccactaat
    30361 gccgatggta aatgggatga caactggggc acctgtacat tacaggactg tagcggcaac
    30421 cgcagccagt cggattccgg tgccagcgtg gcggtcggct ggcgaaatga cgtctggagc
    30481 tgggatatcg gtaccacgcc gatgggcttc aacgtggtgg atgtggtcgg cggcatcagt
    30541 tacagcgatg atatcgggcc gctgggttac accgttaacg cccaccgtcg gcccatctcc
    30601 agttctttgc tggcctttgg tgggcaaaaa gactccccga gcaataccgg gaaaaaatgg
    30661 ggtggcgtac gtgccgacgg tgtggggcta agtctgagct acgataaagg tgaagcaaac
    30721 ggcgtctggg catcgcttag tggcgaccag ttaaccggta aaaatgtcga agataactgg
    30781 cgcgtgcgct ggatgacggg ctattactat aaggtcatta accagaacaa tcgccgcgtc
    30841 acaatcggcc tgaacaacat gatctggcat tacgacaaag atctgagtgg ctactcactc
    30901 ggtcagggcg gttactacag tccgcaggaa tacctgtcgt ttgccatacc ggtgatgtgg
    30961 cgggagcgca cggaaaactg gtcgtgggag ctgggggcgt ctggctcgtg gtcgcattca
    31021 cgcaccaaaa ccatgccgcg ttatccgctg atgaatctga tcccgaccga ctggcaggaa
    31081 gaagctgcgc ggcaatccaa cgatggcggc agcagtcagg gcttcggcta cacggcgcgg
    31141 gcattacttg aacgacgtgt tacttccaac tggtttgttg gcacggcaat tgatatccag
    31201 caggcgaaag attacgcacc cagccatttc ctgctctacg tacgttattc cgccgccgga
    31261 tggcagggtg acatggattt accgccgcag ccgctgatac cttacgccga ctggtaagtt
    31321 ttcagatagc gcctctctta atgccgctgc gatcgggtat actcgggcgg caatctggga
    31381 tttccggggg gagacaattt gcgcgtaagt cgctcgttaa caatcaagca gatggcaatg
    31441 gtggcagccg ttgtcctggt gttcgttttt attttttgca ccgttttgct gttccatctg
    31501 gtccagcaga atcgctataa cacggctacg caactggaaa gcattgctcg ctctgtccgc
    31561 gaacccttat cttcagctat tttgaaaggc gatattcccg aagcggaagc tattcttgcc
    31621 agcattaaac cggcaggcgt ggtcagccgt gccgatgtag tgctgcctaa ccagttccag
    31681 gcgctgcgta aaagttttat tccagagcgc ccggtgccgg taatggttac tcgcctgttt
    31741 gagctaccgg ttcaaatctc gctgggcgtt tactcgctcg aacgtccggc aaacccgcag
    31801 ccaattgcct atctggtact acaggcggat tccttccgta tgtataagtt cgtgatgagc
    31861 accctctcaa cgttagtgac catttactta cttttgtcgc ttatcctgac cgtcgccatc
    31921 agctggtgca ttaaccgcct gattttgcat ccgttacgca atattgctcg cgaacttaac
    31981 gccatcccag cccaggagct tgttggtcac caactggcat taccgcgtct gcatcaggac
    32041 gatgaaatcg gtatgttggt gcgcagttac aacctcaacc agcaattgct gcagcgccat
    32101 tatgaagaac agaacgaaaa tgcgatgcgc ttcccggtgt cggatttgcc gaacaaagcc
    32161 ttgctgatgg agatgctgga gcaagttgtc gcgcgtaaac aaaccaccgc gctgatgatc
    32221 atcacctgtg aaaccctgcg tgatactgcg ggcgtgctga aagaggcgca acgagaaatt
    32281 ctgctgctga cgctggtgga aaaactcaaa tcggtactgt cgccacgtat gatcctcgcg
    32341 cagattagcg gttatgactt tgctgtcatt gccaacggtg tacaggaacc gtggcacgca
    32401 atcaccttag gtcagcaagt gctcactatc atgagcgagc gcctgccgat tgaacgtatt
    32461 caactccgtc cgcactgtag cattggcgtg gcgatgttct acggcgatct caccgccgaa
    32521 cagctttaca gtcgcgctat ttctgcggca tttaccgctc gccataaagg caagaatcag
    32581 attcagttct ttgatccgca gcagatggaa gccgcccaga agcggttgac ggaagagagc
    32641 gatatcctta atgcactgga aaatcatcag tttgctattt ggttacagcc acaggtcgag
    32701 atgaccagcg gtaaactggt cagtgcggaa gtgttactgc gtatccagca accggatggc
    32761 agttgggacc tgccggatgg cttaatcgat cgcattgagt gctgtgggct gatggttacc
    32821 gtcggtcact gggtgctgga agagtcctgt cgattgcttg cagcctggca agagcgcggc
    32881 attatgctgc ccttgtcggt aaacctctct gcgctgcaac tgatgcaccc gaatatggtg
    32941 gcggatatgc tggaactgtt aacccgctat cgcattcagc cgggaacact gattctggaa
    33001 gtgacagaaa gccgacgtat tgacgaccct catgctgcgg tggcaatcct ccgtccgctg
    33061 cgcaatgccg gagttcgggt ggcgctggat gatttcggca tgggctacgc agggctgcgt
    33121 cagctgcagc atatgaaatc gttgccaatc gacgtactga aaatcgacaa aatgtttgtt
    33181 gaaggcttgc cggaagatag cagcatgatt gctgcaatta tcatgctggc gcagagcctg
    33241 aacttacaaa tgattgccga aggcgtggag actgaagcac aacgcgactg gctggcaaaa
    33301 gcgggcgttg gtattgccca gggcttcctt tttgctcgcc cactccctat tgaaatcttc
    33361 gaagagagtt acctggaaga aaagtagcta ccccaaactg attacaaaac tttaaaaagt
    33421 gctggtttgt gcgagccagc tcaaactttt taaccttttt gtttcaatta tgatccaggt
    33481 acatttctgt gatgttgtct gggtgttatt ttaaggccgc aggtacccca taaccttaca
    33541 agacctgtgg ttttactaaa ggacacccta tgaaaacctc tctgtttaaa agcctttact
    33601 ttcaggtcct gacagcgata gccattggta ttctccttgg ccatttctat cctgaaatag
    33661 gcgagcaaat gaaaccgctt ggcgacggct tcgttaagct cattaagatg atcatcgctc
    33721 ctgtcatctt ttgtaccgtc gtaacgggca ttgcgggcat ggaaagcatg aaggcggtcg
    33781 gtcgtaccgg cgcagtcgca ctgctttact ttgagattgt cagtaccatc gcgctgatta
    33841 ttggtcttat catcgttaac gtcgtgcagc ctggtgccgg aatgaacgtc gatccggcaa
    33901 cgcttgatgc gaaagcggta gcggtttacg ccgatcaggc gaaagaccag ggcattgtcg
    33961 ccttcattat ggatgtcatc ccggcgagcg tcattggcgc atttgccagc ggtaacattc
    34021 tgcaggtgct gctgtttgcc gtactgtttg gttttgcgct ccaccgtctg ggcagcaaag
    34081 gccaactgat ttttaacgtc atcgaaagtt tctcgcaggt catcttcggc atcatcaata
    34141 tgatcatgcg tctggcacct attggtgcgt tcggggcaat ggcgtttacc atcggtaaat
    34201 acggcgtcgg cacactggtg caactggggc agctgattat ctgtttctac attacctgta
    34261 tcctgtttgt ggtgctggta ttgggttcaa tcgctaaagc gactggtttc agtatcttca
    34321 aatttatccg ctacatccgt gaagaactgc tgattgtact ggggacttca tcttccgagt
    34381 cggcgctgcc gcgtatgctc gacaagatgg agaaactcgg ctgccgcaaa tcggtggtgg
    34441 ggctggtcat cccgacaggc tactcgttta accttgatgg cacatcgata tacctgacaa
    34501 tggcggcggt gtttatcgcc caggccacta acagtcagat ggatatcgtc caccaaatca
    34561 cgctgttaat cgtgttgctg ctttcttcta aaggggcggc aggggtaacg ggtagtggct
    34621 ttatcgtgct ggcggcgacg ctctctgcgg tgggccattt gccggtagcg ggtctggcgc
    34681 tgatcctcgg tatcgaccgc tttatgtcag aagctcgtgc gctgactaac ctggtcggta
    34741 acggcgtagc gaccattgtc gttgctaagt gggtgaaaga actggaccac aaaaaactgg
    34801 acgatgtgct gaataatcgt gcgccggatg gcaaaacgca cgaattatcc tcttaatctc
    34861 atcacttatg cccgcactcc cttccccgag tgcgggtgtt tgcgcataat tgacccctgt
    34921 gcctgaactt accacctggg tgagttcagg tttatttcac ttcttccgtg acattttcat
    34981 gttcttgcgg tctaacacga agtgttttta acgtcattta gactgaccga caacgggcag
    35041 tttttttatt accaggatag ttgatcaggg gttcacatgc agggcacaaa aattcgactt
    35101 ttagcgggcg gtttgctgat gatggccact gctggctatg tgcaggcaga tgcgctccag
    35161 cctgatccag catggcaaca ggggacgctt tccaacggtt tacagtggca agtgctgacc
    35221 accccccagc gtcccagcga tcgtgttgaa attcgcctgc tggttaatac cggttcgctc
    35281 gccgaaagta cacaacagag cggttacagt cacgccatcc ctcgtattgc gctaacgcaa
    35341 agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc agcaggggat cgaccctaaa
    35401 cgcccgatgc cgccggtaat tgtctcttat gacaccacgc tgtttaatct gagtttgccc
    35461 aataaccgta acgatttgct gaaagaagcg ctctcttatc tggcaaatgc cactggcaaa
    35521 ttgaccatca caccagaaac catcaaccac gcgctgcaaa gtcaggacat ggtggcaacc
    35581 tggccagccg atactaaaga gggctggtgg cgctatcgtc tgaaagggtc aaccttgtta
    35641 ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag cggaaaaaat taaagatttc
    35701 tatcagaaat ggtacacccc ggatgcaatg acgctactgg tggtgggaaa cgtggatgcg
    35761 cgctcggttg tcgaccaaat aaacaaaacg tttggcgaac tgaaaggcaa acgtgaaacg
    35821 ccagctccgg tgccgacgct ttctccgctg cgtgcggaag cggtgagtat tatgactgac
    35881 gcggtgcgtc aggaccggtt atctatcatg tgggatacgc cgtggcagcc gattcgtgaa
    35941 tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc gcgaggcgct gttctggcat
    36001 gttcagcaag cgttaagcgc cagtaacagc aaagacatcg gtcttggatt tgactgccgt
    36061 gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat caccaaacga caagctgaac
    36121 agcaacctta atctggtggc gcgtgaactg gcgaaggttc gcgataaagg tctgccggaa
    36181 gaagagttca atgcgttagt ggcgcaaaag aaactggagc tgcagaaact gtttgccgcc
    36241 tatgcgcggg ctgataccga tattctgatg ggtcagcgga tgcgttcgtt gcaaaatcag
    36301 gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc aggatttcct taacagcctg
    36361 acggtggaga tgttaaatca ggatctgcgt cagcagttgt cgaatgatat ggcgttaata
    36421 ctgctgcagc cgaaaggcga gccggaattt aacatgaaag cgttgcaggc ggcctgggat
    36481 caaatcatgg ccccatcgac tgctgctgct gccgccacct ctgtcgccac ggatgacgta
    36541 catcctgaag tgacggatat tccacctgca cagtaatgcc tgatggtgat gagttagcac
    36601 aatcatcagg tatttatgga tgagctggat agaggggtaa cactttatcc agccttttgc
    36661 atatgctgct tttacgctgg catcgcctca cgcgggataa tcgcgccgcg atactgaata
    36721 acggtacttg cggtcaggtg cccgcgtttc gccgcgtttt ccgcactgcc gcccgttaaa
    36781 cgtaccgcca gataaccggc actgaaagag tcgccagctg cggtggtatc gatcactttt
    36841 tctttcggca gtttcaccgc cggaacatcc actaaccctt cgccagcaat ggacaccagg
    36901 caagaatccg ccccgcgttt caccaccact tctttcacgc ccgcgttatg ggtgcgcgca
    36961 atgacgtctt ccaccggctg ttgaccccac agcgcgtctt catcgtccag cgtcaggaag
    37021 gcgatatccg tgcattccag catttgttgg tacacctgct gtgtctcttc tttgctggcc
    37081 cacaggcgcg gacgatagtt attgtcgaaa atcacttttc cgccgttggc gcggcattcg
    37141 cgcagcaggg aaagcagctt ttcgcggctg gtcgggctta agatcgccag gctaatcccg
    37201 ctcaggtaga gataatcgaa attcgccagc tcttcgcaaa tcgccgcaga ctgctcactc
    37261 tccagccaga atttggcggc ggcttcgttc cgccagtagt agaacgtacg ctcgccggtg
    37321 ctgtcggttt caatgtagta aaggcccggc agacggtttt ccatccgttg ggtcagggaa
    37381 gtatcaacgt tctcgccgtg ccaggcgtcc agcatttgct ggctaaaact gtccgttccc
    37441 agcgccgtta cgtaatgaac ggttaatgcc gcaggatcga cctgacgggc gatatagacg
    37501 gaagtgttca gggtatcgcc gccgaaaccg cgcttaacgt ccgcgccttt ctcggaaagc
    37561 tcaatcatgc attcgccaat cacggcaatc tttttggaca tagtcgtgaa cctgatctgt
    37621 aaaaaattag cgttagtttg cgctgtggtg gtttgctggt caaccatatt aaaacagtgt
    37681 tccattattt ttttgagcca gaacgtattc ctgaaagatt ccgttgtgga gtgaggaaat
    37741 ttaccccggc caggcggtaa agttctgccc ttgcgcgccg ataatctttg tcgagtccgg
    37801 gcagcatcac ttttaaacac aggacatctt tgatgataag gcaggttatc cagcgaataa
    37861 gcaaccctga agcaagcatc gagagcttgc aggaacggcg tttttggttg cagtgtgagc
    37921 gtgcttacac ctggcagccg atctatcaaa catgcggacg gttaatggcc gtggagctat
    37981 taacggtggt cacgcatccc ttgaaccctt cgcaacgcct gccgccggat cgctatttta
    38041 ctgaaatcac cgtcagccat cggatggagg ttgtgaaaga gcagattgat ttgctggcgc
    38101 aaaaagccga cttctttata gagcacggcc tgctggcatc ggtcaatatt gatggcccta
    38161 cgctcatcgc cctgcgtcag caaccaaaaa tcctgcgcca gattgagcgt cttccctggc
    38221 tgcgtttcga actggtggag catatccgtc tgccgaaaga ttcaaccttt gcctcgatgt
    38281 gtgaatttgg cccgctgtgg ctggatgatt ttggtaccgg gatggcaaat ttctctgcac
    38341 taagtgaagt gcgttatgac tacatcaaaa tcgcgcgaga actgtttgtg atgctgcgtc
    38401 agtcgccgga aggacgcaca ctcttttctc agcttttaca tctaatgaat cgctattgtc
    38461 gcggggtgat tgttgagggc gtagaaacgc cggaagagtg gcgtgatgtt cagaactcgc
    38521 ccgcattcgc cgcacaaggc tggtttcttt cacgcccggc accgatagaa acgctgaata
    38581 cggcggttct ggcgctataa gctgcctcat tttccgcctg gctggactat ctttaggact
    38641 ggcacaggaa gaatatgagg aagcgaacga tgagcaaggc aggcaaaata accgctgcga
    38701 tttcaggggc tttcttgttg ttgattgtcg tggcgatcat tttgattgca acttttgact
    38761 ggaatcgact caaaccgacc atcaaccaga aagtctctgc ggagttgaat cgtccgttcg
    38821 ctatccgtgg cgatctgggc gtggtgtggg agcggcaaaa gcaagaaaca ggctggcgca
    38881 gctgggtgcc gtggccccat gtacacgcgg aagacatcat tcttggcaat ccaccggata
    38941 ttcccgaagt cacgatggtg catttgccac gcgtagaggc aacgctggcc ccgctggcgc
    39001 tgctgaccaa aacggtctgg ctgccgtgga tcaagctcga aaagcccgac gcgcgcctga
    39061 ttcgcctctc cgaaaagaac aataactgga cgtttaatct cgccaacgat gataacaaaa
    39121 acgcgaatgc aaagccgtcg gcatggtcgt ttcggctgga taatattctt ttcgatcaag
    39181 ggcggatcgc cattgatgac aaagtaagca aagcggatct ggagattttt gttgatccgt
    39241 taggcaagcc gctgccgttc agcgaagtta ctggatcgaa aggtaaagcg gataaagaaa
    39301 aggtgggcga ttacgttttt ggcctgaagg cgcagggacg ttataacggt gaaccgctca
    39361 cgggtacggg aaaaataggc ggtatgctgg cgctgcgtgg cgaagggacg ccgtttccgg
    39421 tacaggctga tttccgctct ggtaacaccc gtgttgcttt tgatggcgtc gtgaatgacc
    39481 caatgaagat gggcggtgtc gatttacggc ttaaattttc tggcgattca ctgggtgatc
    39541 tctatgaact gacgggcgtt ctgctgcccg ataccccgcc gtttgaaacg gatggtcggc
    39601 tggtagcgaa aatcgacact gaaaaatcgt cggtctttga ttatcgcggt tttaatgggc
    39661 gaattggcga tagcgatatc cacggttctc tggtctacac caccggaaag ccacgaccaa
    39721 aactggaagg tgatgtcgag tcgcggcaat tgcggcttgc ggacctggga ccgttaattg
    39781 gcgttgactc cgggaaaggg gcagaaaagt cgaaacggtc tgaacagaag aagggcgaaa
    39841 aaagcgttca gcctgcgggc aaagtgctgc cttatgaccg cttcgaaacc gataaatggg
    39901 acgttatgga tgccgatgtt cgcttcaaag ggcggcgcat tgagcatggc agtagcctgc
    39961 cgattagcga tctttctact catatcatcc tcaaaaatgc tgacctgcgc ctgcaaccgc
    40021 tgaaatttgg catggcgggc ggcagcattg cggcgaatat tcatctggaa ggcgataaaa
    40081 agccgatgca ggggcgggca gatattcagg ctcgtcgact gaaactgaaa gaactgatgc
    40141 ccgatgtgga actgatgcag aagacgctgg gggaaatgaa cggtgacgcg gaactacgcg
    40201 gtagcggtaa ctcggtggca gcacttttag gcaacagtaa cggcaacctg aaactgttga
    40261 tgaatgacgg gctggtgagc cgcaacctga tggagattgt tgggctgaat gtcggcaact
    40321 acattgtcgg tgcgatattt ggtgacgatg aggtgcgggt gaactgcgcg gcggcgaatc
    40381 tgaatattgc caacggcgtg gcgcgcccgc agatttttgc tttcgatact gagaacgcgt
    40441 tgattaatgt taccggcacg gcaagttttg cttcggaaca gctggatttg actattgatc
    40501 cggagagtaa agggattcgg attatcacac tgcgttcgcc gctgtatgtg cgtgggacgt
    40561 ttaaaaatcc gcaggctggg gtgaaagccg ggccgttgat tgcccgtggt gctgttgctg
    40621 cggcactggc aacgctggta acaccggcgg cggcgttact ggcactgatc tcaccttccg
    40681 aaggggaggc taatcagtgt cggacgatat tgtcgcagat gaagaagtga tgaggttgga
    40741 ttgtgtttgc tggtgagttc gggagcagcg tgttcccctc accctaaccc tctccccaaa
    40801 ggggcgaggg gactgttttg tgcgtgttga aacaacgagt accgcttttc cgttcacttt
    40861 ctgggggagg gctgttatgt tcgcgatata acatctcgct ccgcttttct ccctctccct
    40921 ttcagggaga gggctggggt gagggtggct gcccacacga tacttacaac gactgatgtc
    40981 gcgtctcatg ggtcagcagc agggcgatta acgtcaagcc agccatcgcc gccagatata
    41041 accccaccgc acctaacccg tagttagtct gcaaccaggc tgcgatatac ggcgcaacgg
    41101 aagccccgag aatcgacgct acgttgtaag agaacgatgc tccggtgtaa cgcacttctg
    41161 ttgggaacag ctctggtaac agcgcgccca tcggcccgaa ggtcagcccc atcagactta
    41221 accccagcag caggaaggca aaaaccagaa tcgggttgcc agaaccgagc agcgggttaa
    41281 aggcgaacag cgcgaacagg atgatcagcg tggtgataat taccatgctt ttacggcgac
    41341 caaaggcatc agccagtaat ccggcgactg gcaccatcac gccaaaacca ataactgcca
    41401 tcatcagcat ccacaacact tcgttacgtg gcaggccaag cccaactggc gcggcggcgg
    41461 tactgaaggt catagagtag accgtcatga tgtaaaacag cgtatatgtt gccagcatga
    41521 tgaacgtacc cagtacggtt acgcgaacat gtttggtcag cagcgtaccc agcgggatct
    41581 tcacctgttt tttcgcttta gcgactttct caaacaccgg cgactcatgc agcgacacgc
    41641 gaacatacag gccgataatg accagcaccg ccgagaagat aaatggcaca cgccagcccc
    41701 agctcataaa ctgctcatcg gtcagtagcc aggaaagcag caagaaagtg ccgttggcaa
    41761 agaagaagcc aatcggtgcg cccagctgtg ggaaggagcc atacagtgca cgtttgcgcg
    41821 gtggggcgtt ttcagtcgcc agcagcgccg cgccgcccca ttcaccgcct aagcccagac
    41881 cctgaccaaa tcgagccagc gccagcagca gtggggcgaa aataccaatc gtggcatagc
    41941 ccggcagcag accaatcacc acggtcgaaa tccccatcgt tagcaacgag gcgaccagcg
    42001 tcgctttacg cccaacgcga tcgccaaaat gaccaaaaac ggcagagcca atggggcgcg
    42061 cgacgaaggc gatggcgaag gtggcgagcg actgtagcgt tgctgccgtt ggatcgccct
    42121 gcgggaagaa gatatgcgga aacacaataa cggccgcagt ggcgtaaatg taaaagtcga
    42181 agaactcaat ggccgtgcca atgagagagg cgacaaggac tttattacgc gagttgatcg
    42241 gcgtgtattc ttgctcgtgg tcgagtgttg tggctgttgc ttgcataatc ttttcttatt
    42301 ttttggctaa cgaatagcca tattacgcac agcaaaagtg atatttcaat gtgtaacaaa
    42361 tcgcggaaat ggtcaaaaac caggcaaaaa ggggataatg tttaggccag tgatttttct
    42421 tctatgaaat gcttcacata attttataaa tagtggataa aactggtttc aggttaaata
    42481 aaagttacca actggattta tatgctgggg ttatgagttg gaatgaaatt gtttgctccc
    42541 aaatagatgg ctgaagaggc tctggggaag agtttcaaga ggttatgtgc gcattatagc
    42601 cggatgcgat gctggcgcat cttatccggc ctacggggag ccaggccgaa taaaatcgta
    42661 tgccggataa ggcgtttacg ccgcatccgg cctattttaa ggctgcgttt tccctggcat
    42721 tcgcgggtcg tctttatatt cggcggtggc aatccacgcc gcgcaaaaca gcgtcaaacg
    42781 ggcgaagaag tagaaaaatg ccatcagccc cagcacggaa ccaaatgctg cgccagaggg
    42841 ggatttcatc agcgatggca gggtgtaggt catcacgatt ttaatcactt caaaaccaat
    42901 agcagccaga aatgttccgc gaatcagggc ttttttgcgc ggacggtggc gcggtaaccg
    42961 ccagaagatc cagaagaaaa gcagatagtt ggcgaagatg gaaatcgcca ggccaatcaa
    43021 ccgccacgtc ggtttcagcc actcaatgct gttcaggtgc agggcgctaa taatcatttg
    43081 ctgcgccgaa ccggcaaccg aggtgatcga aagcgtcaca atcagcgcaa tcaacaaacc
    43141 aatcagcgaa ataaaatcac gcagatattt tacccagaac ttctcctgat cttgcggcga
    43201 gcgttcccag acatcgcgcg actgggcacg aatcgcttca cgcaggttac ccatccagtt
    43261 gatgccggaa taaagcgcca ccgccaggcc gacaagccct acagtcgtac gctgctgaac
    43321 ggcggtgttg atggtgtttt tcaacgtggc ggctagcgtc ggatcgctga tgttttgcag
    43381 aattttgtcg aagatatcct gtagcaacat cggatgggag gccagcacaa agccccctgc
    43441 ggcaaacgac accatcaaaa tcgggatcat cgataaaaat gagaaatagg tgatagccgc
    43501 accaaactgg ttacccagcc gatcgttaaa gcgttctgtc gcgcgaatca ggtgcgcaat
    43561 aaccggttgg cgttggactt tttcggcagt ggtggtgacg gtttccagcg cctggctaac
    43621 tttgccgcct ttctcgctat tatccaactg cttaatcggc tcgtgctcca gatcctgggt
    43681 gggacgtttg atctcgtttt cctgcgtcat ccgctctttt gtccttctgt cgtggtcatt
    43741 cttaaaaagt atagtcggtc agtccaggtg ctctttcatt acgccagcca gccactgcat
    43801 aaacaggttt acacgccggg aaagctcccg acgctgcggg taaaccagcg aaagggagag
    43861 cggctcggca cggtagcctg gcaacacttc aataagccgc ccggcacgca gggcttcgcg
    43921 cacggcaatg cgcgggatct gaataatccc cagaccggca agaccggcgg cgagatagtt
    43981 ttcgctactg tttaccgtca acatgccgcc agacttaaac cactggacgc cattaacgct
    44041 ggcaacctca aaacctaacg gatgtacacc caggtgcggt gtataacgca ctattgcgtg
    44101 tgaagtcaga tcatcggggc tttgcggata accaaagcgc gtcagatagt gcggactggc
    44161 acagttgacc atggtcagtt tgccgagggg acgggcgata acgccatctt ccggtaacgc
    44221 gccagtgcgt atcacgcaat caaaaccatc atgaagaata tctaccggac ggtcatggct
    44281 actcagttcc agctcaattc ccggatgcag atagagaaat tccgacaggc gcggcagtaa
    44341 caggcttttc gcgattcctg gcgggatgtc gatgcgtaat ttaccgctga tactggtggc
    44401 atcctgttga aacagaccgt ccagttcgct gagattactc aacacatctt ttgctcgttg
    44461 ataataggtc atgccttctg gcgtgagttt gacccgtcgc gtggtgcgct ggagaagctg
    44521 ggtgccaagt tgatgttcca gtgcctgtat ctggcgcgaa acacttcctt ttggcaaagc
    44581 aaagaaatcc gccgcgcggg aaaaactttc cagctccgcg actttgatga acaactgcat
    44641 tgcgtgaatt ttatccattt ctttgaccga ttgttgttca cagcgaaaca gtaacgcgca
    44701 tatcgcattg tttattgttt tttctaacat ctaatgccgg gtttcacaat gttgtcatga
    44761 cgaatgtggc gatttctcag caggaaaagg gcggaatagc tttctgagaa aggtgaatcc
    44821 ccctaaacag agcgtatttt tcagcgattc cctttaaagt gaatagagga cgtgttggct
    44881 atttgtgctt atttttgtct cattgccgtt tgttattttt gcttgtggtc gcggagtttc
    44941 atttttaatt gcatgcgtac taaccaccaa tataaggaaa tcctcaaaat tgttttaaat
    45001 agtgccctcg acatgtaatt tcaggcgttt agctgtaact atttatatcg gtaactattt
    45061 tctacgcgtc atctctgtag aatggccacc gccaaatggc gaattcttat tttttctcgc
    45121 gggtaatacc cttccactac ttaagtcttt ttaacgtatt caggtggaga ttcagttatg
    45181 caaatagtca tgtttgacag gcagtcaata tttattcatg gaatgaaaat cagtttacag
    45241 cagcgtattc caggagtgag tattcagggg gccagtcagg cagacgagtt atggcaaaag
    45301 ctggaaagtt accctgaagc cttagttatg ctcgatggcg atcaggatgg tgagttttgc
    45361 tactggttgc tgcaaaaaac cgtggtgcag tttcctgaga ttaggtgtta atcacggcga
    45421 cggattgcaa taaacggtgg ttacaggaag ttattcattt taatgtgctg gccattgtgc
    45481 ctcgtgattc aaccgtcgag acatttgcgt tggcggtaaa tagtgcggcg atggggatga
    45541 tgtttctgcc tggagactgg cggacgacgc cggaaaagga cattaaagat ctcaaatcgt
    45601 taagcgcccg ccagcgggag attttaacca tgttagcggc gggcgaatca aataaggaga
    45661 tcagcagagc gctgaatatc agtaccggaa cggtaaaagc gcatctggaa tccctgtatc
    45721 gccgtctgga agtgaaaaat cgcacccagg cggcaatgat gttaaatatc tcctcctgaa
    45781 gaggataatt ttacagccag caggaagtta gcggctggct gtaaaaatat tatggttcgc
    45841 cgtacaaacc aattaaacgg cgtaccacac cgttagtcca gccaaaccca tcctgcaacg
    45901 gatactcgcc accgccgcct tcgcggggaa caccatcggc aatatggtat ttctcgatca
    45961 ttttgtgctg ttccagatag aactgattca ccgttttcag ccagctgcgc gcgatttcat
    46021 cacccagaag gtcatcgcca tacattttaa atccctgaat tgccatccat tgtaacggtg
    46081 cccagccatt gggtttatcc cactgttcac cggtttcgta ctcgcttgcc agaatcccgc
    46141 caggtgtcag taaccggctg cgtacggcgt ttgccagacg atcggcctgt tcatggttcg
    46201 ccatgccgac atagagcggc acaatggcgg cagcggaaaa cagcgccagt tgttcgcgtc
    46261 gccagtcgta atcgcggtag atgccgtttt catcatccca gaggtaacgg tttaccgcat
    46321 cgcgacgggc actggccttc tggcggaaca gcgcttctgt ctctttctcg cctttcagcg
    46381 ccgagatgtt ggcgatggcg ctctccagtt taaacaggaa ggcattcaga tcgatgggga
    46441 tgaactgggt ggtacgaatg ctcgccagac gaccagtatc acgcagccaa cgggaagagt
    46501 aatcccaacc tgaggccgct cccgcgcgta aatcgcggta cacctcgttg ggcgggcgac
    46561 cagaatgttt cgcggtttca acgtcctcaa gccaggattc gtcacgcggc gtgtcgcgat
    46621 cgtcccaata acggttgagc agcgatccgt ccggcatccg cacaacatgg cgataggcct
    46681 gattagggat caacgattct gcaccgtcca tccagaaggc atattccatt ttaaggtggt
    46741 cgagatagcg gcgcgcaccg cgtacaccat cttcttcaaa caactccacc atcagcgcaa
    46801 aaaccggtgg ttgcgatcgg ctcaaataat aggtgcggtt gccgttgggg atgtgaccat
    46861 agttttcgat catccaggcg aagttatcgg ccatgcattt cagcaaatct tcccgaccac
    46921 tttccgccag ccccagcatg gtgaaatagg aatcccaata gtaggtttcg ctaaaacggc
    46981 cgcccgggac aatatatgac tgcggtagcg ccagcagaga agaccacgga atgtgatcct
    47041 gtggttcgcg ggttagcacc ggccacagct ggtcgatatg ctctttcagg gaattttgcg
    47101 ggtccgatac atactcgctg gagtagacct ccggcagcca gaagtgattt tcaacaaact
    47161 tgcgcaagtc aaaatcacga tgacggcgca ctttacggta gcggattaaa atatccagcg
    47221 ggtccatttt gggtgcgcag tcgggaaagg ttttactgtc ggggaaaatt ttcgccgact
    47281 gaacatgctc gaacagttcg agatagcgat cggcaggcgt cagcgcatca gaggcgggca
    47341 gcccttcaat catctcgggt tccggttctg cctcgatcat ctcatccagt tttaattcat
    47401 acgggtccag ctcatagcag agatcgactt cgatcatcag ttcgtctgga ttagggtttt
    47461 gaattttctg attgagcata aggatccaac ctccgaaatt cgtcgctaat tatcgggctt
    47521 ttctttaagg gtagtcattc gctccgcctg gtgggcgaca aagcgtgcgg tggatcacgt
    47581 ttattaacct gtcaaaaaat ataacgcact aatatccatg gaatttataa aaatacggat
    47641 ttcgtgttgt ttttcatttt cagaacattc aatcctgctc tcgccgcaat catgatatgc
    47701 cattcctgat ctgggtaata ggcacaggct atcttattga tagtttatat tcatgtaatt
    47761 gattgattcc tatcaaaaag cgtatctccc tgttttttat gatggctttg tctcgcagag
    47821 acgtttgcag cattctggaa aattttctgg gtcacaataa ctcaccaccg tgagggaaga
    47881 ataatgaaaa tggtctcacg tattaccgcg atcggcctgg ctggcgtcgc gatttgctat
    47941 ttagggttat ctggttatgt gtggtaccac gataataaac gcagtaaaca ggccgatgtt
    48001 caggcatctg ctgtcagtga aaataataag gttttaggct ttctccgcga aaaaggatgc
    48061 gactattgcc acacaccttc ggcagaatta cccgcctatt attatattcc tggcgcgaaa
    48121 cagttgatgg attacgacat taagcttgga tataaatctt ttaacctcga ggccgtgcgt
    48181 gcggcactgc tggctgataa acccgtttcg caaagcgatc tgaataagat tgaatgggtg
    48241 atgcagtatg aaactatgcc accaacgcgt tataccgcgc tacactgggc gggtaaggtg
    48301 agtgatgaag agcgggcgga aatactggcc tggattgcaa aacagcgcgc ggaatattac
    48361 gccagcaatg atactgctcc ggagcatcgc aatgaaccgg tgcagcccat cccgcaaaaa
    48421 ctgcctaccg atgcgcaaaa agtggcgttg ggctttgcgc tgtatcacga tccccgttta
    48481 tcggctgata gcaccatttc atgcgctcat tgccatgcgt tgaatgcggg gggcgtcgat
    48541 ggcagaaaaa catcgattgg tgttggtggc gcagttgggc cgattaacgc gccgacggta
    48601 tttaactcag tatttaacgt tgagcagttc tgggatggtc gtgcggcaac attgcaggat
    48661 caggcaggtg gaccgccgtt gaacccgatt gaaatggcgt cgaaatcctg ggacgaaatt
    48721 attgctaagc tggaaaaaga tccgcagctt aaagcgcagt tcctcgaagt ttatccgcaa
    48781 ggtttcagtg gcgaaaatat tactgatgcc attgctgaat ttgagaaaac attaattacg
    48841 ccggattccc catttgataa atggttgcgt ggagatgaaa atgcgctgac ggcgcaacag
    48901 aaaaaaggct atcaattatt taaagataat aaatgtgcaa cttgtcatgg tggtattatt
    48961 ctcggcggac gttcctttga accgttgggg ctgaaaaaag actttaactt tggggaaatt
    49021 acggcggcgg atattggtcg tatgaatgtg actaaagaag agcgtgataa attgcgtcag
    49081 aaagtacccg gtttacgtaa cgttgcttta acggcaccgt acttccatcg cggtgacgtg
    49141 ccgacgctgg acggggcggt gaaactgatg ctgcgctatc aggtaggcaa agagctgccg
    49201 caggaggatg tggatgatat cgtagctttc ctgcacagtc tgaacggggt gtacacgccg
    49261 tatatgcagg ataaacaata attaatttga tcgcccgaac aggaatgttt gggcgatttt
    49321 tattacgata ataaagtctg tttttaatat tatcatgtta aatgtttata ttataaaaag
//
LOCUS       DAZGSP010000024        48956 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZGSP010000024 DAZGSP010000000
VERSION     DAZGSP010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.024"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2699
                     /gene="cas3"
                     /locus_tag="ACHK74_001540"
     CDS             <1..2699
                     /gene="cas3"
                     /locus_tag="ACHK74_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFP0220385.1"
                     /translation="RKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLDV
                     AAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPE
                     LKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTG
                     HHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWY
                     ISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLIQAQNALTVFPLKAKVAPFNGIN
                     TLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQG
                     LFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVG
                     SEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKI
                     LLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQQ
                     EAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQG
                     KCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKYC
                     SLQRASQVIVCTQVIEQSVDIDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDG
                     KDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMP
                     HAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYL
                     PEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLE
                     GEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
     gene            2797..4359
                     /gene="casA"
                     /locus_tag="ACHK74_001541"
                     /gene_synonym="cse1"
     CDS             2797..4359
                     /gene="casA"
                     /locus_tag="ACHK74_001541"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFP0220386.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDISAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALQSLEHAFQFGPDSPSFM
                     QDFEALTGDKVPVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWINVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDEQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLDKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVDLERVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            4356..4892
                     /gene="casB"
                     /locus_tag="ACHK74_001542"
                     /gene_synonym="cse2"
     CDS             4356..4892
                     /gene="casB"
                     /locus_tag="ACHK74_001542"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFP0220387.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            4904..5959
                     /gene="cas7e"
                     /locus_tag="ACHK74_001543"
     CDS             4904..5959
                     /gene="cas7e"
                     /locus_tag="ACHK74_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFP0220388.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKVLAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            5970..6716
                     /gene="cas5e"
                     /locus_tag="ACHK74_001544"
     CDS             5970..6716
                     /gene="cas5e"
                     /locus_tag="ACHK74_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFP0220389.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            6698..7348
                     /gene="cas6e"
                     /locus_tag="ACHK74_001545"
     CDS             6698..7348
                     /gene="cas6e"
                     /locus_tag="ACHK74_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFP0220390.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRWEELQGAFRFFVLSQERPAESDTFTIECRSFAPELRTGQSLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            7345..8265
                     /gene="cas1e"
                     /locus_tag="ACHK74_001546"
     CDS             7345..8265
                     /gene="cas1e"
                     /locus_tag="ACHK74_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFP0220391.1"
                     /translation="MTFVPLSPIPLKDRTTMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            8265..8558
                     /gene="cas2e"
                     /locus_tag="ACHK74_001547"
     CDS             8265..8558
                     /gene="cas2e"
                     /locus_tag="ACHK74_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFP0220392.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGKNRRIPVDLDGLRLVSFLPVDNQ"
     repeat_region   8655..9598
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=8655..8683
                     /rpt_unit_seq="gtgttccccgcgccagcggggataaaccg"
     gene            complement(9680..10717)
                     /gene="iap"
                     /locus_tag="ACHK74_001548"
     CDS             complement(9680..10717)
                     /gene="iap"
                     /locus_tag="ACHK74_001548"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFP0220393.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            10969..11877
                     /gene="cysD"
                     /locus_tag="ACHK74_001549"
     CDS             10969..11877
                     /gene="cysD"
                     /locus_tag="ACHK74_001549"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFP0220394.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            11879..13306
                     /gene="cysN"
                     /locus_tag="ACHK74_001550"
     CDS             11879..13306
                     /gene="cysN"
                     /locus_tag="ACHK74_001550"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFP0220395.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIRKDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            13306..13911
                     /gene="cysC"
                     /locus_tag="ACHK74_001551"
     CDS             13306..13911
                     /gene="cysC"
                     /locus_tag="ACHK74_001551"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFP0220396.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            13961..14284
                     /gene="ygbE"
                     /locus_tag="ACHK74_001552"
     CDS             13961..14284
                     /gene="ygbE"
                     /locus_tag="ACHK74_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFP0220397.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            14478..14789
                     /gene="ftsB"
                     /locus_tag="ACHK74_001553"
     CDS             14478..14789
                     /gene="ftsB"
                     /locus_tag="ACHK74_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFP0220398.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            14808..15518
                     /gene="ispD"
                     /locus_tag="ACHK74_001554"
     CDS             14808..15518
                     /gene="ispD"
                     /locus_tag="ACHK74_001554"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFP0220399.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            15518..15997
                     /gene="ispF"
                     /locus_tag="ACHK74_001555"
     CDS             15518..15997
                     /gene="ispF"
                     /locus_tag="ACHK74_001555"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFP0220400.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            15994..17043
                     /gene="truD"
                     /locus_tag="ACHK74_001556"
     CDS             15994..17043
                     /gene="truD"
                     /locus_tag="ACHK74_001556"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFP0220401.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            17024..17785
                     /gene="surE"
                     /locus_tag="ACHK74_001557"
     CDS             17024..17785
                     /gene="surE"
                     /locus_tag="ACHK74_001557"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFP0220402.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKESLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            17779..18405
                     /gene="pcm"
                     /locus_tag="ACHK74_001558"
     CDS             17779..18405
                     /gene="pcm"
                     /locus_tag="ACHK74_001558"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFP0220403.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            18590..19684
                     /gene="nlpD"
                     /locus_tag="ACHK74_001559"
     CDS             18590..19684
                     /gene="nlpD"
                     /locus_tag="ACHK74_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFP0220404.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            19747..20739
                     /gene="rpoS"
                     /locus_tag="ACHK74_001560"
     CDS             19747..20739
                     /gene="rpoS"
                     /locus_tag="ACHK74_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFP0220405.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(20860..21267)
                     /locus_tag="ACHK74_001561"
     CDS             complement(20860..21267)
                     /locus_tag="ACHK74_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP0220406.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            21414..22007
                     /locus_tag="ACHK74_001562"
     CDS             21414..22007
                     /locus_tag="ACHK74_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFP0220407.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            22007..23434
                     /locus_tag="ACHK74_001563"
     CDS             22007..23434
                     /locus_tag="ACHK74_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFP0220408.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIVLHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            23445..23681
                     /locus_tag="ACHK74_001564"
     CDS             23445..23681
                     /locus_tag="ACHK74_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFP0220409.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(23720..25084)
                     /gene="ygbN"
                     /locus_tag="ACHK74_001565"
     CDS             complement(23720..25084)
                     /gene="ygbN"
                     /locus_tag="ACHK74_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFP0220410.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(25173..25949)
                     /gene="ygbM"
                     /locus_tag="ACHK74_001566"
     CDS             complement(25173..25949)
                     /gene="ygbM"
                     /locus_tag="ACHK74_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFP0220411.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            complement(25954..26592)
                     /gene="ygbL"
                     /locus_tag="ACHK74_001567"
     CDS             complement(25954..26592)
                     /gene="ygbL"
                     /locus_tag="ACHK74_001567"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFP0220412.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(26589..27851)
                     /gene="otnK"
                     /locus_tag="ACHK74_001568"
     CDS             complement(26589..27851)
                     /gene="otnK"
                     /locus_tag="ACHK74_001568"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFP0220413.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(27848..28756)
                     /gene="ltnD"
                     /locus_tag="ACHK74_001569"
     CDS             complement(27848..28756)
                     /gene="ltnD"
                     /locus_tag="ACHK74_001569"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFP0220414.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            28952..29719
                     /gene="ygbI"
                     /locus_tag="ACHK74_001570"
     CDS             28952..29719
                     /gene="ygbI"
                     /locus_tag="ACHK74_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFP0220415.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(29770..30426)
                     /gene="pphB"
                     /locus_tag="ACHK74_001571"
     CDS             complement(29770..30426)
                     /gene="pphB"
                     /locus_tag="ACHK74_001571"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP0220416.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPRSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(30532..33093)
                     /gene="mutS"
                     /locus_tag="ACHK74_001572"
     CDS             complement(30532..33093)
                     /gene="mutS"
                     /locus_tag="ACHK74_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP0220417.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            33169..33348
                     /locus_tag="ACHK74_001573"
     CDS             33169..33348
                     /locus_tag="ACHK74_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220418.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVG
                     EPFFFRVIVPFKIKM"
     gene            33380..33733
                     /gene="ygbA"
                     /locus_tag="ACHK74_001574"
     CDS             33380..33733
                     /gene="ygbA"
                     /locus_tag="ACHK74_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFP0220419.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(33770..35848)
                     /gene="flhA"
                     /locus_tag="ACHK74_001575"
     CDS             complement(33770..35848)
                     /gene="flhA"
                     /locus_tag="ACHK74_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFP0220420.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRALQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(35922..36932)
                     /gene="hypE"
                     /locus_tag="ACHK74_001576"
     CDS             complement(35922..36932)
                     /gene="hypE"
                     /locus_tag="ACHK74_001576"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFP0220421.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(36929..38050)
                     /gene="hypD"
                     /locus_tag="ACHK74_001577"
     CDS             complement(36929..38050)
                     /gene="hypD"
                     /locus_tag="ACHK74_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFP0220422.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(38050..38322)
                     /gene="hypC"
                     /locus_tag="ACHK74_001578"
     CDS             complement(38050..38322)
                     /gene="hypC"
                     /locus_tag="ACHK74_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFP0220423.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(38313..39185)
                     /gene="hypB"
                     /locus_tag="ACHK74_001579"
     CDS             complement(38313..39185)
                     /gene="hypB"
                     /locus_tag="ACHK74_001579"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFP0220424.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(39189..39539)
                     /gene="hypA"
                     /locus_tag="ACHK74_001580"
     CDS             complement(39189..39539)
                     /gene="hypA"
                     /locus_tag="ACHK74_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP0220425.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            39759..40220
                     /gene="hycA"
                     /locus_tag="ACHK74_001581"
     CDS             39759..40220
                     /gene="hycA"
                     /locus_tag="ACHK74_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFP0220426.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            40345..40956
                     /gene="hycB"
                     /locus_tag="ACHK74_001582"
     CDS             40345..40956
                     /gene="hycB"
                     /locus_tag="ACHK74_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFP0220427.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSVDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            40953..42779
                     /gene="hycC"
                     /locus_tag="ACHK74_001583"
     CDS             40953..42779
                     /gene="hycC"
                     /locus_tag="ACHK74_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFP0220428.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVTRLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            42782..43705
                     /gene="hycD"
                     /locus_tag="ACHK74_001584"
     CDS             42782..43705
                     /gene="hycD"
                     /locus_tag="ACHK74_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFP0220429.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLSQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVSGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            43723..45432
                     /gene="hycE"
                     /locus_tag="ACHK74_001585"
     CDS             43723..45432
                     /gene="hycE"
                     /locus_tag="ACHK74_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFP0220430.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            45442..45984
                     /gene="hycF"
                     /locus_tag="ACHK74_001586"
     CDS             45442..45984
                     /gene="hycF"
                     /locus_tag="ACHK74_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFP0220431.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            45984..46751
                     /gene="hycG"
                     /locus_tag="ACHK74_001587"
     CDS             45984..46751
                     /gene="hycG"
                     /locus_tag="ACHK74_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFP0220432.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            46748..47158
                     /gene="hycH"
                     /locus_tag="ACHK74_001588"
     CDS             46748..47158
                     /gene="hycH"
                     /locus_tag="ACHK74_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFP0220433.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            47151..47621
                     /gene="hycI"
                     /locus_tag="ACHK74_001589"
     CDS             47151..47621
                     /gene="hycI"
                     /locus_tag="ACHK74_001589"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFP0220434.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEVE"
     gene            47664..48419
                     /locus_tag="ACHK74_001590"
     CDS             47664..48419
                     /locus_tag="ACHK74_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220435.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQNWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGVLQNMAGNAQLYRAD
                     LDRNGIQDLVIWRGISGNGLAPNAFLILMTFNQQGRPCVFQSDGFYTASETGIDDLLD
                     LQRNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLYGVNQN"
     gene            48490..48909
                     /locus_tag="ACHK74_001591"
     CDS             48490..48909
                     /locus_tag="ACHK74_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0220436.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
BASE COUNT        11419 a        12550 c        13436 g        11551 t
ORIGIN      
        1 tgcgtaaata tcctttaagt ttactgaagg ataaaaatat tgtgactttt tttgatttct
       61 ggggaaaaac ccgacgcggc gagaaagacg ggggcgacga ctatcacctt ctttgctggc
      121 attcgctgga tgtagccgcg atgggctatt taatggttaa aagaaattgc ttcgggctgg
      181 ctgattattt tcgtcaatta gggatttctg acaaggaaca ggcggctcaa tttttcgcct
      241 ggttgctgtg ctggcacgat attggaaaat ttgcccgctc ttttcaacaa ctttacctgc
      301 cccctgaact caagattcag gaaggtgcca gaaaaaatta cgaaaaaatc tctcattcaa
      361 cgctgggcta ctggttgtgg aatcattatt taagtgaatg tcaggagtta cttccttcat
      421 cttcgctctc tccgcgcaaa cttaggcgag tgatagaaat gtggatgccc gtgacgacag
      481 ggcatcatgg tcggccccct gatcgtatgg atgaattgga taattttctg cctgaagaca
      541 aagctgcagc acgagatttt ctccttgaaa tcaaaccact gtttccgctc atagagatcc
      601 ccgcattctg ggatgatgat gagggcattg aacttataaa acacctttcc tggtatatct
      661 ctgcaaccgt cgtgctcgca gactggacgg gttcgtcaac gcgatttttt ccacgcgtcg
      721 cacacccaat ggatattaaa ggttactggc agaaaacttt aattcaggct caaaacgcct
      781 taaccgtctt tcctctaaaa gcaaaagtcg cgcctttcaa cggaattaat acgctgtttc
      841 cttttattga gaatccgaca ccgttacagc aaaaggtgct ggatctggat atcagccagc
      901 aaggaccgca gttatttatt ctggaagatg tgactggcgc aggtaaaaca gaagcggcgc
      961 ttatcctggc gcacaggttg atagctgcgg ggaaagctca ggggctgttt tttggtctgc
     1021 caacaatggc aacggccaat gccatgtacg atcggctggt caaaacctgg cttgctttct
     1081 attcgccaga atcccgcccc agcctggtgc tggcacatag tgcccgcaca ttaatggacc
     1141 gctttaatga atcactctgg tctggtgatt tagtcgggtc agaagaaccg gatgaacaaa
     1201 cgttcagtca gggatgtgcg gcctggtttg ccgacagtaa caagaaggcg ttgctggctg
     1261 aaattggcgt cggcacgctg gatcaggcga tgatggcggt gatgccgttc aaacataata
     1321 atctgcggct tctggggctg agtaacaaaa tcctgctggc tgatgagatc cacgcctgtg
     1381 acgcttacat gtcgtgcatt cttgaagggt tgatcgagcg gcaggcgcgt ggcggaaaca
     1441 gcgttatttt gctttctgcc acgttatctc aacagcagcg cgacaaactc gtcgctgcct
     1501 ttgcgcgggg tacagagggc cagcaagaag ctccgttcct ggaaaaggat gattacccct
     1561 ggctgacgca tgtcacgaaa tccgatgtga actcacaccg ggtagcgacg cgcaaagacg
     1621 ttgagcgtag cgtcagcgtg ggttggcttc atagtgaaca agagtgtatt gcgcgtatcg
     1681 aatcggcggt aagtcagggg aaatgcatcg cctggatccg gaattctgtc gatgacgcta
     1741 ttaaggttca tcgtcagctg cttgcccgcg gcgtcattcc tgcttccagc ctttcacttt
     1801 ttcatagccg ctttgctttt agcgatcgcc agcgaattga aacggaaacg ctggcacgct
     1861 ttggtaaata ttgtagttta cagagagcgt cgcaagtcat tgtttgtaca caagtaattg
     1921 agcaaagcgt ggatattgac ctggacgaaa tgatctccga cctggcccct gttgatttgc
     1981 tgattcagcg agcaggacga ttacagcggc atatccgcga tattaatggt cagttaaagc
     2041 gtgacggaaa ggacgagcgt tccccccctg aattgctgat tctggctccc gtctgggacg
     2101 actctcctgg cgacgaatgg tttggcagtg ccatgcgtaa cagtgcattt gtctatcccg
     2161 atcatgggcg aatctggctg acgcagcgtg tgctgcgtga gcaaggtgct attcagatgc
     2221 cacacgcagc ccggcttctt attgaatccg tctacggtga ggacgtggta atgccggaag
     2281 gatttgcccg cagcgagcag gagcaagtgg gcaaatatta ctgtgatcgc gcaatggcta
     2341 aaaagtttgt gctgaacttc aggcccggct atgccgccaa tatcaacgat tacctcccgg
     2401 aaaaactgtc gacacgtctg gctgaggagt ctgtttccct gtggctggct acctgtattg
     2461 acggtgtggt gaagccttat gccaccggtg ctcacgcatg ggaaatgagc gttgtcagag
     2521 tgcgccgaag ctggtggaaa aaacatcggg atgagttttc tttactggag ggagaggcgt
     2581 tcaggctgtg gtgcattgaa cagcgacaag atccggagat ggcaaacgtg attttagtca
     2641 acgatgacga aagttgcggg tattcggcta cggaaggatt gattggcaag gttggttgag
     2701 atgtctgcga caggactctc tgaatagttg atacaccgaa aaggatggaa agatggacaa
     2761 ccgacctggg atggtctttt ttacgagagg atgcgtatga actcgttttc acttctgaca
     2821 accccgtggt tgcccgttcg ttttaaagac ggaacgacag gcaaactggc gccagtcgat
     2881 ctggcggatg aaaatgttgt cgatatttct gcgccgcggg cagatctcca gggtgcggta
     2941 tggcagtttt tgctggggct actacaaacc agcttcgcgc caaaagatca tcgtcgttgg
     3001 gatgatatct gggaagacgg actggaagct gaaaagctac gggaagcatt gcagtcatta
     3061 gaacatgctt tccagtttgg gccagattcc ccttcattta tgcaggattt cgaggcgctc
     3121 accggagata aagttccggt tgcttcgcta ctgcctgaga ttcccggcgc tcaaacaacg
     3181 aagtttaata aagaccactt tatcaagcgt ggtgtaacgg aatacctgtg cccacattgt
     3241 tctgcgttag ctctgttctc cctacagttg aatgcgccgt caggtggcaa aggctaccgc
     3301 accggtttac gcggcggtgg gccgatgacc actctgattg aattacagga atatcagggt
     3361 aatcaacaaa ctcccttgtg gcgcaaactg tggatcaatg tcatgccgca ggatgaagcc
     3421 gatttaccgc tacccaaaaa atttgacgat ctggttttcc cctggcttgg cccgacgcgc
     3481 accagcgaac tggccggtgc ggtggtaacc gatgagcagg tcaataaact ccaggcgtac
     3541 tggggaatgc ctcgccgtat tcgtattgat tttaatacca cgacagtcgg caactgcgat
     3601 atttgcggtg agcagagcga cgcgctcctg agcctgatga cgaccaaaaa ttacggtgcg
     3661 aattatgcca tgtggcagca tcccttaacg ccttaccgtg taccacttaa agagggtggc
     3721 gagttttact ccgttaaacc acaaccgggc ggtttaatct ggcgtgactg gttaggcctt
     3781 atcgaaacgg gtaaatcaga aaacaatacg gaacttcccg cgctggtggt gaaactcttc
     3841 aatgccagca gtctcaagca ggcaaaagtg ggcctgtggg gatttggcta tgatttcgac
     3901 aacatgaaag cgcgctgttg gtacgaacac catttcccgc tgctgctcga taaaaaagaa
     3961 ggtcagatac cgaagctgcg gctggctgcg caaacagctt cacggattct aagtctgtta
     4021 cggagtgcat tgaaagaagc atggttctcc gatccaaaag gtgcaagggg tgattttagt
     4081 tttgtggata tcgacttctg gaacaaaact cagcatcgct ttctgaggtt agtgcgccaa
     4141 attgaagaag gtcaggatgc ggatgaatta ctcagcaaat ggaataagga aatttggtta
     4201 ttcgcacgtc aggattttga cgagcgtgta ttcaccaatc cttatgagcc cgttgatctg
     4261 gaacgcgtca tgaccgcgcg caagaaatat tttacaacat cggcggagaa gcaaagtgct
     4321 aaagccgcca gggagaaaaa gcaggaggct gctgaatgag tattgtaaaa gaggagcaca
     4381 aagccacgct gcgcaaatgg cacgaggaat tgcaggagaa acgtggcgaa cgtgccagtc
     4441 tgcggcgtag tacgacggtt aatgatgttt gtctgacgga tggatttcgc ctgtttctga
     4501 agaacagaca aataaagtgg caggatgaac ctgagtggcg tatcaccgca ctggcgctga
     4561 tagccgcagt tagcgccaat gttaaagcta ttgatgaacg gcagcctttt gccgcgcagc
     4621 tggcggcggt aatgtcaaag gggcgtttta ctcgtctgtc ggcagtaaaa acaccggatg
     4681 aattactgcg ccagttacgc cgcgcagtca gattattaaa cggttccgtc aatcttgact
     4741 ctctggcaga gggggtcttc cgttggtgcc aggagagcga cgacctgctc aatcatcacc
     4801 gccgccagca gcggccaacc gaatttatcc gtatccgctg ggcgctggaa tattaccagg
     4861 ctggcgacgc cgataacgaa caaaatcaat aaaggactat ctcatgacaa catttattca
     4921 gcttcatttg ttaaccgctt accctgctgc caaccttaac cgtgatgata ccggtgcgcc
     4981 aaaaactgtg gtgctgggtg gagcaacgcg tctgcgcgtt tcctcgcaaa gtctgaaacg
     5041 tgcgtggcgc acttctgcac tttttgaaca ggcactggcg ggccatattg gtattcgcag
     5101 tgggcgtatt gcgcgtgagg cggcaactat cctgattgag aaaggaatcg aagagaaaaa
     5161 agccatcgaa tgggcggcaa aaattgcaga ttatcttggg aaagctaaaa acgacaaaaa
     5221 accaaaagat ccgctcacta acgccgaaac tgaacagtta gtccatatca gcccggcaga
     5281 atttgacgcc gtaaaagtgc tggcccatca gcttgcagaa gaaaagcgcg cgccaaaaga
     5341 ggaagatctc gcgttgttac gcaaagaccg tatggcggta gatattgcca tgtttggtcg
     5401 tatgctggcg aataaacccg agtttaatgt tgaagccgcc tgccaggtag cgcacgcatt
     5461 tggtgtcagt gaaacgattg tcgaagatga ttttttcacc gccgttgatg atttgcgcca
     5521 ggcttctgaa gatgccggtg ccgggcatct tggtgaaacc ggattcggtt ctgcactgtt
     5581 ctacacctat atctgcattg ataaagatct gctggtcgaa aaccttggcg gtgacgaagc
     5641 gttagctaat cagaccatac gtgcctttac ggaagccgca cttaaagtct ccccaaccgg
     5701 caaacagaac agctttgcca gccgtgccta cgcctcctgg gcgctggcag aaaaaggcac
     5761 cgatcaacca cgctctctgg cggcggcttt ctatgaaccc attaatggca cacgtcagtt
     5821 agatgtggcg gtgcagcgta ttacaacgct tcgcgaaaac atgaatacgg tctacgaaca
     5881 gaagaccgaa tgcgcaagtt ttgacgtgat gaacaaacag ggaagcatga aggacgtgct
     5941 ggactttatc tgcgcgtaag gggcggctta tgagccaata tctgattttt cagcttcatg
     6001 ggccgatggc gtcctggggg gtcgatgctc caggtgaagt tcgtcatacc catgaacttc
     6061 cttcgcgctc agcattgctg gggctactgg ctgccggggt agggattcgg cgtgatgata
     6121 ccgaacgctt aaacgcgttt aatcgccact attcactggt ggtttgcgcc agccgtaacc
     6181 cgcgctgggc acgggattat cacacggtcc agatgccaaa agaggtgcgt aaagcacgtt
     6241 atttcagccg tcgcgaagag ttgagcgacc ctgagctttt gagcgcgatt atctcccggc
     6301 gcgactacta caccgatgcc tggtggatgg tggcggtggc aacaacagcc gatgcgcctt
     6361 acagcctcga acagttgcag gacggtttac gccatccagt ttttccgctt tatctggggc
     6421 gaaaaagtca tccgctggca ttacctcttg cgccgttact gctcgaaggc aacgcgtgtg
     6481 atgccttatg taatgcatac caacagtatc aggatcattt ccataaactg aaagtctcac
     6541 tcccgaaact tcaggatgaa tgctggtggg aagggaaaca cgacggcctg gtcgcgagca
     6601 aaatattacg ccgccgggat gttcctttaa atcgtcagca gtggctgttt ggggaacgca
     6661 ccgtcaatca ggggccgtgg ctcagcaagg aggaaccatg tacctctcaa gaataacctt
     6721 acataccggc cagctttcgc ctgcgcagtt gctgcatctg gtggatcgcg gtgaatatgt
     6781 aatgcatcag tggctgtggg atctcttccc tggcggcaaa gaaaggcaat ttctttatcg
     6841 ttgggaagaa ctccagggcg cgtttcgctt ttttgtactg tcgcaggaac gcccggcgga
     6901 aagtgatacg ttcaccatag aatgccggtc atttgcgcca gagttgcgta ccggacaatc
     6961 cctttgtttc aacctgcggg cgaatccaac aatctgtaaa gcgggcaagc gccacgatct
     7021 gctgatggag gcgaaacggc aggtgagggg gcaggcggaa ggaagtgatg tctggttgca
     7081 tcaacaacag gcggcgctgg actggctggc agcgcagggg gagagaagcg gttttacact
     7141 gctggatacc tcggttgatg cctaccgaca acagcaactg cggcgggaaa actcccggca
     7201 actgatccag ttcagtagcg tggattatac ggggatgctt acggtcaccg atcctgggtt
     7261 atttctgcaa cgtctgagcc aggggtatgg caaaagccgg gcgttcggct gtggtctgat
     7321 gctgatcaaa cccggagcag aggcgtgacg tttgtaccac tgagcccgat cccattaaaa
     7381 gatcgcacca ccatgatctt cctccagtac ggtcagatcg acgtactgga cggcgctttc
     7441 gtgctgatcg acaaaacagg gatccgcaca catattccgg taggatcggt cgcctgcatt
     7501 atgctcgaac cgggaacgag agtttcccac gcggcggtgc atctggccgc cacggtggga
     7561 acactgctgg tctgggtcgg tgaagcgggc gttcgcgttt actcatccgg acaacccgga
     7621 ggggcgcggg cagataaatt actctaccag gcaaagcttg ctttaacgga agatcttcgg
     7681 ttgaaggtgg tgcgcaaaat gtatgaatta cgttttcgtg agccaccgcc agctcgccgt
     7741 tcagtggagc agttacgtgg aattgaggga tctcgcgttc gccagacgta tgcattactg
     7801 gcgaaacaat atggtgtgaa atggaatggt cgcaaatacg atcctaaaga ctgggaaaaa
     7861 ggcgatgttg tgaatcgctg catcagtgct gctacatcat gtctgtacgg tatatctgaa
     7921 gcggcagtat tagccgcggg atatgcgccc gctattgggt ttatccatag tggcaaaccg
     7981 ctttcatttg tttatgacat agccgatatc attaaatttg attcggttgt gccaaaggca
     8041 tttgaaatag cagcgaggca acccgcagaa cctgataaag aagtcagatt agcctgtcgc
     8101 gatattttcc gtagcactaa gttaacgggc aaattaatac cgttaattga ggaagtcctt
     8161 gccgcaggtg aaattgaacc gccacaacct gcgccggata tgttaccgcc agccatcccc
     8221 gaacctgaaa cgttgggcga tagcggtcat cgaggacgcg gctgatgagc atggtcgtgg
     8281 ttgttacaga aaatgtcccg ccgcgcttac gtggacggct cgcaatctgg ctactggaag
     8341 tgcgtgccgg tgtgtatgtt ggtgatacat caaaacgtat tcgggagatg atctggcagc
     8401 aaattaccca actggcaggt tgtggaaatg tggtcatggc ctgggcgaca aatactgagt
     8461 ctggttttga gtttcagacc tggggcaaaa acagacgtat tccggtggat ttggatgggt
     8521 tacgtttggt ttcttttctt cctgttgata atcaataagt tagacgttct ttaaaaataa
     8581 ggaaatgttt gaatttagtt ggtagattgt tgatgtggaa taaatttgtt taaaaacaga
     8641 tatgtatgct tagtgtgttc cccgcgccag cggggataaa ccggcggtgg cgatgcaggc
     8701 ggcgcagtgg cgcatgtgtt ccccgcgcca gcggggataa accgatcaga ccgtttttga
     8761 agtgggcagg cggaaagtgt tccccgcgcc agcggggata aaccgtcttt cgtcaatttt
     8821 ggcgctataa acttcatgtg ttccccgcgc cagcggggat aaaccgcgcc catcagcacc
     8881 gcgcaggatc aggaaaccgt gttccccgcg ccagcgggga taaaccgcgg cccgcgcggc
     8941 cgcaattgag gccggtttgg tgttccccgc gccagcgggg ataaaccgcc acacacatcg
     9001 agctggtggg gattaatgct gtgttccccg cgccagcggg gataaaccgt tttccaccag
     9061 tcgcagtggg agcaaaaact ggtgttcccc gcgccagcgg ggataaaccg ggaatccacc
     9121 gcgtcggcta tggcctgata gtgtgttccc cgcgccagcg gggataaacc gttaacgcga
     9181 aagcgtcggc ggtcagtcgg caggtgttcc ccgcgccagc ggggataaac cggaatatcg
     9241 ggtgccagcc tggacgctgg ccgagtgttc cccgcgccag cggggataaa ccgcaaaatt
     9301 taaaatcatt taagcttgta aaaccgtgtt ccccgcgcca gcggggataa accgggtaca
     9361 cggatattct gtctaaaagt taactagtgt tccccgcgcc agcggggata aaccgactga
     9421 taacgccgga gccttctttt tcagcaagtg ttccccgcgc cagcggggat aaaccgaggt
     9481 tgacgttgat tttgttcgtt atgttgccgt gttccccgcg ccagcgggga taaaccggcg
     9541 tctcgagcgc gggacgattc aaaaccagcg tgttccccgc gccagcgggg ataaaccggg
     9601 cgcactggat gcgatgatgg atatcactta gaattccccg cccctgcggt agaactccca
     9661 gctcccattt tcaaacccat caagacgcct tcgccagctc cttcaccaac ggtagcatta
     9721 tccgcataac gtcacggcag cgacgttcta ttcttccagg aagtgcctta tcaatatgct
     9781 gttgattatc cagtcttacg tcatgccagc tatttcccgc agggaatgcg gctgtttttg
     9841 cgcgttgctg ataaccatcc ttattcccaa gattccagtt agtcgcttcc accgaaagta
     9901 cagcaatgcc cgctttgtcg aatatttctg cgtcattaca acacccagtg cctttcggat
     9961 aatttttatt caaacccgga ttggtcgttg cggctattcc gtgactgcgc gcaattgcca
    10021 gcgccctgtc gcgcgttaat ttccttactg cttcaggggt ttttacaccg ctgttgaaat
    10081 acaatttatc gccaacaatt aagttatcga gattaatcac cagcagcgta tttttctttt
    10141 cggtgtcact catccgcttg agtaaattct cagcgcctaa tttcccttcc tcttcgccgc
    10201 tggtcgccac aaatcgaata ccatactcgg taggcgtatt tttcaggcgt tctgccaatt
    10261 ccagcatgac acctaaacct gcggcgttat catccattcc ttgtaacgtc agcccgccga
    10321 gattggcatc ggcgtcagca tcgctcagcg gggcgtaagt atccagatgc gccataatga
    10381 tgatctgctg cggcgctttg ccttcatgag cggcaatcac cgtacttcct gtcacgttat
    10441 gccagttctt gcgattatcg cgggcggtat aaatataccg actattaaat gtccgaatat
    10501 cactgcgata acccatttgc tgaaactgtt ggcgaatata atcggcagat aacatttctg
    10561 ctggagttcc ggtcatgcgt cccggaaaga aagtagcaat atgtcgtgcc tgggtattag
    10621 caaaatcgcc aggtttaggt gacgaggcgt gtacggggag aataaagcat acgccgagcg
    10681 ccagggcagc ggtacggtgg cgcaatgcgg aaaacatagt gagtccttaa ataccatgca
    10741 aattttttta ccgccatagt atgaaactgc cgctgcgcta aaacaatttc aaatcttcct
    10801 aaacgcccga aatccggtgc cttaagcact ttttgatatt agctttgcca aatcgttatt
    10861 ccgttaagga actactcatt ctaattggta atttcattcg ttctcttacg ctccctatag
    10921 tcgaaacatc tgatggcaag aaaatagcgg tattgcaaag gaacggttat ggatcaaaaa
    10981 cgacttactc acctgcggca actggaggcg gaaagcatcc acattattcg cgaggtggcg
    11041 gcagaattct caaatccggt gatgctctac tctatcggta aagattccag cgtcatgctg
    11101 catttggcgc gcaaggcgtt ttatccaggt acgctgcctt tccccttgct gcatgtcgat
    11161 accggctgga aattccgcga gatgtatgag ttccgcgatc gtacggcgaa agcctacggc
    11221 tgcgagctgc tggtgcataa aaacccggaa ggcgtggcga tggggattaa tccattcgtg
    11281 cacggcagcg cgaaacatac cgatattatg aagaccgaag gcctgaagca ggcgctgaat
    11341 aaatacggtt ttgatgccgc cttcggtggt gcgcgccgcg acgaagagaa atcccgtgct
    11401 aaagagcgta tctactcttt ccgtgatcgc ttccatcgct gggacccgaa aaatcagcgc
    11461 ccggagctgt ggcacaacta taacgggcag attaacaaag gcgaaagcat ccgcgttttc
    11521 ccgctctcta actggaccga gcaggatatc tggcaataca tctggctgga aaatatcgac
    11581 attgtgccgc tgtatctcgc tgcggaacgt ccggttctgg aacgcgacgg tatgttgatg
    11641 atgattgatg acaaccgtat cgacctgcaa ccgggcgaag tgattaaaaa acggatggtg
    11701 cgtttccgta cgctgggctg ctggccgctg accggtgcgg tggagtcaaa tgcacaaaca
    11761 ctgccggaga tcatcgaaga gatgctggtt tccaccacca gtgaacgtca gggccgcgtg
    11821 attgaccgcg accaggcggg gtctatggag ctgaaaaaac gtcaggggta tttttaagat
    11881 gaacaccgca cttgcacaac aaatcgccaa tgaaggcggc gtcgaagcct ggatgattgc
    11941 gcaacaacat aaaagcctgc tgcgttttct gacctgtggt agcgtcgatg acggcaaaag
    12001 taccctgatt ggtcgcctgc tgcacgatac ccgccaaatc tacgaagatc agctctcatc
    12061 gctgcataac gacagtaagc gtcacggcac ccagggcgaa aaactggatc tggctctgct
    12121 ggtggacggc ctgcaagctg agcgcgaaca gggcatcact attgatgtgg cctaccgcta
    12181 tttctctacc gagaagcgta aatttattat cgccgacacc cccgggcacg agcagtatac
    12241 ccgcaatatg gcgaccggcg cctcaacgtg tgagctggcg atcttactga ttgatgcccg
    12301 taaaggcgtg ctcgatcaaa cccgtcgtca cagttttatc tccacgctgc tggggatcaa
    12361 acatctggtc gtagcgatca acaaaatgga tctggtggat tacagtgaag agacattcac
    12421 ccgcattcgt aaagactacc tgactttcgc cgaacagtta cctggcaatc tggatattcg
    12481 ctttgtgccg ctctctgcgc tggaaggcga caacgtggct tcgcaaagtg aaagtatgcc
    12541 gtggtacagc ggtccgacac tgctcgaagt gctggaaacc gtggagatcc agcgagtggt
    12601 ggatgctcag ccaatgcgct tcccggtgca gtacgttaat cgcccgaatc tcgattttcg
    12661 tggttacgcc ggaacgctgg catccggtcg cgtggaagtc gggcaacgtg taaaagtgct
    12721 gccttctggt gtggaatcaa acgtcgcgcg gatcgtgact tttgatggtg atcgcgaaga
    12781 agcctttgcc ggagaagcga tcaccctggt gctgacggat gagatcgaca tcagccgtgg
    12841 cgatctgctg ctggcggcag acgaagcgtt accggcggtg cagagcgcgt cggtggatgt
    12901 ggtatggatg gcggaacagc cgctttctcc agggcagagt tacgacatca aaattgccgg
    12961 taagaagacg cgcgcgcgtg ttgatggcat tcgctatcag gttgatatta ataaccttac
    13021 ccagcgtgaa gttgaaaacc tgccactgaa tgggatcggc ctcgtggatc tcacttttga
    13081 cgagccgctg gtgttagatc gttatcaaca aaatccggtg acgggtgggc tgatttttat
    13141 cgatcgcctg agcaatgtga ccgtgggtgc cggtatggtg cacgagccag ttagccaggc
    13201 aactgctgcg ccatctgaat tcagtgcatt cgaactggaa ttgaatgctc tggttcgtcg
    13261 ccactttccg cactggggcg cgcgcgattt gctgggggat aaataatggc gctgcatgat
    13321 gaaaacgtcg tctggcatag ccatccggtc actgtgcaac aacgcgagct acaccacggt
    13381 catcgtggtg tagtgctgtg gtttaccggc ctctccgggt ccggtaaatc aacggtcgcc
    13441 ggggcgctgg aggaggcgtt acataaactc ggcgtcagta cgtatctgct ggatggcgat
    13501 aatgttcgcc acggattatg cagcgatctc ggttttagcg atgccgatcg taaagagaat
    13561 atccgtcgcg tcggtgaagt ggcgaatttg atggttgaag ccggactggt agtgctgacc
    13621 gcatttatct cgccacaccg cgccgaacgc cagatggttc gcgaacgcgt aggagaaggg
    13681 cgctttatcg aagtgtttgt cgatacgccg ctggcgattt gcgaagcccg cgatccaaaa
    13741 ggcttatata agaaagcgcg tgccggtgaa ctgcgcaact ttacgggaat agattccgtt
    13801 tacgaagcgc ctgaatcggc agaaattcat ctcaatggtg aacaattagt aacaaatttg
    13861 gtacagcaat tattagatct gttgagacag aacgatatta tcagatcctg agacaccacc
    13921 gggtttccat gcccggttcg tcaaagtaac aggatcaaat atgcgtaata gccataacat
    13981 tacactaaca aataacgaca gccttactga ggatgaagaa accacatggt cactgcctgg
    14041 tgccgtggtc ggttttatct cctggttatt tgcgctggcg atgccgatgt tgatttatgg
    14101 ctctaacacg ctgttcttct ttatctacac ctggcctttc tttctggcgc tgatgcccgt
    14161 cgcggtagtg gtggggattg cgctgcattc attgatggac ggaaagctac gctacagtat
    14221 tgttttcacc ctggtgacag taggcattat gtttggcgca ctgtttatgt ggctactggg
    14281 ctaatttgta ctttcttgtc tcctctgttc ataattcaaa ccgtaactaa taatgagatt
    14341 atgttctgca cgccctgggt atacgtaaca atggacaaat gtggtacatt tgcccgcgtt
    14401 gtcgcggtat ccccaacaga ggatgtagag tcgtcttcgg atgcatggga tgatgatgcc
    14461 gtttttcagg gggcaggatg ggtaaactaa cgctgctgtt gctggctatt ctggtctggc
    14521 tacagtattc gctgtggttc ggtaagaacg gtatacatga ctatacccgc gtcaatgatg
    14581 atgtggcggc acagcaagct acaaacgcga aacttaaagc gcgaaacgat caactttttg
    14641 ccgaaattga cgatctcaat ggcggccagg aggcgctcga agagcgtgcg cgtaatgaac
    14701 tcagcatgac caggccgggc gaaacttttt atcgtctggt gcctgacgcg tcgaagcgcg
    14761 cacagtctgc ggggcaaaac aatcgataaa tcagcccggg aattaacatg gcaaccactc
    14821 atttggatgt ttgcgccgtg gttccggcgg ccggatttgg ccgtcgaatg caaacggaat
    14881 gtcctaagca atatctctca atcggtaatc aaaccattct tgaacactcg gtgcatgcgc
    14941 tgctggcgca tccccgggtg aaacgtgtcg tcattgccat aagtcctggc gatagccgtt
    15001 ttgcacaact tcctctggcg aatcatccgc aaatcaccgt tgtagatggc ggtgatgagc
    15061 gtgccgattc cgtgctggca ggtttgaaag ccgctggcga cgcgcagtgg gtattggtgc
    15121 atgacgccgc tcgtccttgt ctgcatcagg atgacctcgc gcgattgttg gcgttgagcg
    15181 aaaccagccg cacgggaggg atcctagccg caccagtgcg cgatacgatg aaacgtgccg
    15241 aaccgggcaa aaatgccatt gctcataccg ttgatcgcaa cggcttatgg cacgcgctga
    15301 cgccgcaatt tttccctcgt gagctgttac atgactgtct gacgcgcgct ctaaatgaag
    15361 gcgcgactat taccgacgaa gcctcggcgc tggaatattg cggattccat cctcagttgg
    15421 tcgaaggccg tgcggataac attaaagtca cgcgcccgga agatttggca ctggccgagt
    15481 tttacctcac ccgaaccatc catcaggaga atacataatg cgaattggac acggttttga
    15541 cgtacatgcc tttggcggtg aaggcccaat tatcattggt ggcgtacgca ttccttacga
    15601 aaaaggattg ctggcgcatt ctgatggcga cgtggcgctc catgcgttga ccgatgcatt
    15661 gcttggcgcg gcggcgctgg gggatatcgg caagctgttc ccggataccg atccggcatt
    15721 taaaggtgcc gacagccgcg agctgctacg cgaagcctgg cgtcgtattc aggcgaaggg
    15781 ttataccctg ggcaacgtcg atgtcactat catcgctcag gcaccgaaga tgttgccgca
    15841 cattccacaa atgcgcgtat ttattgccga agatctcggc tgccatatgg atgatgttaa
    15901 cgtgaaagcc actactacgg aaaaacttgg atttaccgga cgtggggaag ggattgcctg
    15961 tgaagcggtg gcgctactca ttaaggcaac aaaatgattg agtttgataa tctcacttac
    16021 ctccacggta aaccgcaagg caccgggctg ctgaaagcca atccggaaga ctttgtggtg
    16081 gtggaagatt tgggctttga gcctgatggt gaaggtgagc atattctggt tagaatcctc
    16141 aaaaacggct gcaatacccg ttttgtggcg gatgcactgg cgaaattcct gaaaattcat
    16201 gcccgtgaag tcagcttcgc tgggcaaaaa gacaaacatg ctgttacgga acagtggtta
    16261 tgcgctcgcg tgccaggcaa ggaaatgccc gatctgagcg cctttcaact ggaaggctgc
    16321 caggtgctgg agtatgcgcg gcacaagcgc aagctgcgtt taggcgcgct gaaaggtaac
    16381 gcctttaccc tggttctgcg cgaagtgagc aatcgcgatg acgttgaaca acgtctgatc
    16441 gatatttgcg taaaaggtgt accgaactac ttcggtgccc aacgttttgg gattggcggt
    16501 agcaacttgc agggggcgca gcgctgggcg caaaccaata ctccggtgcg cgatcgcaat
    16561 aaacggagtt tttggttgtc ggcagcccgc agtgcgttgt ttaatcagat tgttgctgag
    16621 cgcctcaaaa aagcagacgt taatcaagtt gttgacggcg atgcgctaca attagccgga
    16681 cgtggtagct ggtttgtcgc aaccaccgaa gaactggcgg aattacagcg tcgcgtcaac
    16741 gataaagagc tgatgataac cgccgcattg ccgggcagtg gcgaatgggg aactcagcgt
    16801 gaagcgctgg cattcgaaca agcagctgtc gccgcagaaa ctgaattaca agctttactg
    16861 gtgcgcgaaa aagttgaagc cgcgcgcaga gcgatgctgc tgtatccgca acaattaagc
    16921 tggaattggt gggatgacgt caccgtagag atccgtttct ggcttccggc gggtagtttt
    16981 gcaaccagcg ttgtcaggga acttatcaac acaacaggtg attatgcgca tattgctgag
    17041 taatgatgac ggggtacatg cacccggtat acaaacgctg gcgaaagcct tgcgtgagtt
    17101 tgctgacgtt caggtggtcg cccccgatcg taaccgcagc ggcgcttcaa attctctgac
    17161 actggaatcc tccctgcgca cgtttacctt tgaaaatggt gatattgctg tgcaaatggg
    17221 aaccccgacc gattgcgtct atcttggcgt gaatgctctg atgcgtccgc gcccggacat
    17281 tgttgtgtcc ggaattaacg ccgggccgaa tctgggggat gatgttattt attccggtac
    17341 ggtagccgcc gcgatggaag gccgtcattt aggttttccg gcgcttgccg tctcgcttga
    17401 cgggcataaa cattacgaca ctgccgcggc ggtaacctgt tcaattttgc gcgcactgtg
    17461 taaagagtcg ctgcgcaccg ggcgtattct taatattaac gttccggatt tacccttgga
    17521 tcaaatcaaa ggtattcgcg tgacgcgctg cggtacacga catccggcag atcaggtgat
    17581 cccgcagcaa gatccgcgcg gcaatacgct gtactggatt ggcccgccgg gcggtaaatg
    17641 tgatgccggt ccggggaccg attttgctgc ggtagatgag ggctatgtct ccatcacgcc
    17701 gctgcatgtg gatttaactg cgcatagcgc gcaagatgtg gtttcagact ggttaaacag
    17761 cgtgggagtt ggcacgcaat ggtaagcaga cgcgtacaag cacttctgga tcaattacgt
    17821 gcgcaaggta ttcaggatga gcaggtgctg aatgcacttg ccgccgtgcc gcgtgaaaaa
    17881 ttcgttgatg aagcgtttga acaaaaagcc tgggacaata tcgccttgcc gataggtcag
    17941 gggcagacaa tttcgcagcc gtatatggtg gcgcgaatga ccgaattact cgagctgacg
    18001 ccgcagtcgc gggtgctgga aattggcact ggttcgggat atcaaacggc aatcctggcg
    18061 catcttgtcc agcatgtttg ctcggtcgaa cggattaaag gtttgcagtg gcaggcgcgt
    18121 cgccgcctga aaaatcttga tttacataat gtttcaaccc gtcatggcga tggatggcaa
    18181 ggttggcagg cacgcgcgcc gtttgacgct atcattgtta cggcggcacc gccggaaatt
    18241 ccaactgcgc taatgacgca gctggacgaa ggcgggattc tcgtcttacc cgtaggggag
    18301 gagcaccagt atttgaaacg ggtgcgtcgt cggggaggcg aatttattat cgataccgtg
    18361 gaggccgtgc gctttgtccc tttagtgaag ggtgagctgg cttaaaacgt gaggaaatac
    18421 ctggattttt cctggttatt ttgccgcagg tcagcgtatc gtgaacatct tttccagtgt
    18481 tcagtagggt gccttgcacg gtaattatgt cactggttat taaccaattt ttcctggggg
    18541 ataaatgagc gcgggaagcc caaaattcac cgttcgccgc attgcggctt tgtcactggt
    18601 ttcgctatgg ctggcaggct gttctgacac ttcaaatcca ccggcaccgg tcagctccgt
    18661 taatggcaat gcgcctgcaa ataccaattc tggtatgttg attacgccgc cgccgaaaat
    18721 ggggacgacg tctacagccc agcaaccgca aattcagccg gtacagcagc cacaaattca
    18781 ggctactcaa caaccgcaaa tccagccagt gcagccagta gctcagcagc cggtacagat
    18841 ggaaaacgga cgcatcgtct ataaccgtca gtatgggaac attccgaaag gcagttatag
    18901 cggcagtacc tataccgtga aaaaaggcga cacacttttc tatatcgcct ggattactgg
    18961 caacgatttc cgtgaccttg ctcagcgcaa caatattcag gcaccatacg cgctgaacgt
    19021 tggtcagacc ttgcaggtgg gtaatgcttc cggtacgcca atcactggcg gaaatgccat
    19081 tacccaggcc gacgcagcag agcaaggagt tgtgatcaag cctgcacaaa attccaccgt
    19141 tgctgttgcg tcgcaaccga caattacgta ttctgaatct tcgggtgaac agagtgctaa
    19201 caaaatgttg ccgaacaaca agccagctgc gaccacggtc acagcgcctg taacggtacc
    19261 aacagcaagc acaaccgagc caactgtcag cagtacatca accagtacgc ctatctccac
    19321 ctggcgctgg ccgactgagg gcaaagtgat cgaaaccttt ggcgcttctg aggggggcaa
    19381 caaggggatt gatatcgcag gcagcaaagg acaggcaatt atcgcgaccg cagatggccg
    19441 cgttgtttat gctggtaacg cgctgcgcgg ctacggtaat ctgattatca tcaaacataa
    19501 tgatgattac ctgagtgcct acgcccataa cgacacaatg ctggtccggg aacaacaaga
    19561 agtgaaggcg gggcaaaaaa tagcaaccat gggtagcacc ggaaccagtt caacacgctt
    19621 gcattttgaa attcgttaca aggggaaatc cgtaaacccg ctgcgttatt tgccgcagcg
    19681 ataaatcggc ggaaccaggc ttttgcttga atgttccgtc aagggatcac gggtaggagc
    19741 caccttatga gtcagaatac gctgaaagtt catgatttaa atgaagatgc ggaatttgat
    19801 gagaacggag ttgaggtttt tgacgaaaag gccttagtag aagaggaacc cagtgataac
    19861 gatttggccg aagaggaact gttatcgcag ggagccacac agcgtgtgtt ggacgcgact
    19921 cagctttacc ttggtgagat tggttattca ccactgttaa cggccgaaga agaagtttat
    19981 tttgcgcgtc gcgcactgcg tggagatgtc gcctctcgcc gccggatgat cgagagtaac
    20041 ttgcgtctgg tggtaaaaat tgcccgccgt tatggcaatc gtggtctggc gttgctggac
    20101 cttatcgaag agggcaacct ggggctgatc cgcgcggtag agaagtttga cccggaacgt
    20161 ggtttccgct tctcaacata cgcaacctgg tggattcgcc agacgattga acgggcgatt
    20221 atgaaccaaa cccgtactat tcgtttgccg attcacatcg taaaggagct gaacgtttac
    20281 ctgcgaaccg cacgtgagtt gtcccataag ctggaccatg aaccaagtgc ggaagagatc
    20341 gcagagcaac tggataagcc agttgatgac gtcagccgta tgcttcgtct taacgagcgc
    20401 attacctcgg tagacacccc gctgggtggt gattccgaaa aagcgttgct ggacatcctg
    20461 gccgatgaaa aagagaacgg tccggaagat accacgcaag atgacgatat gaagcagagc
    20521 atcgtcaaat ggctgttcga gctgaacgcc aaacagcgtg aagtgctggc acgtcgattc
    20581 ggtttgctgg ggtacgaagc ggcaacactg gaagatgtag gtcgtgaaat tggcctcacc
    20641 cgtgaacgtg ttcgccagat tcaggttgaa ggcctgcgcc gtttgcgcga aatcctgcaa
    20701 acgcaggggc tgaatatcga agcgctgttc cgcgagtaag taagcatctg tcagaaaggc
    20761 cagtctcaag cgaggctggc cttttttctt tcggttggtg agtcggtgta tttgtagtct
    20821 ggataagacg cgatagcgtc gcatccggaa atttgccccc tatgcgttca tcatcttgcg
    20881 caccagctgc ataaacagct cctgctcttc tccactcaaa cgccccaaaa attcctcatc
    20941 cacgctgtca ccaatgggta tcgccgcagc aagtatcttt tcgccttcgg cagtcaacca
    21001 gacaaagcgt cgccgcttat cagcggggtc atgttcacgt ctgactaagc cacgattttc
    21061 cattcttgcc aacatttctg ctaacgttgc tttggtgctg acagctgctt ctatcagtgc
    21121 cacctgttcg ataccaggtt tatcagcaat ggcgcgcatc accgcgtact gtggtttggt
    21181 caggtcaggt aactcgtgct gccagcgagc cgtgtgctgc tgaaaaagct gtcgtaactg
    21241 atggaacgct ttatttcgta acgccatgta aactccgggc taattctctg tcgctatcat
    21301 agcggttttt tagtgacgaa gaagatgttg ttacttttca atccttgccg ctgtggaaat
    21361 gaaggagtat gttaataata acgttcgtat acgaacaatt aagaggataa gcaatgaaac
    21421 tgatcgtcgg gatgacaggg gctaccggtg cgcctcttgg tgtggcatta ctgcaagcgc
    21481 tgcgggagat gccgaatgtc gagactcatc tggtgatgtc gaagtgggcg aaaaccacca
    21541 ttgaactgga aacgccttac agcgctcgcg atgttgctgc cctcgcagac ttcagccata
    21601 acccggcgga tcaggcggcg atcatctcat ccggttcttt tcgtaccgac ggcatgatcg
    21661 ttattccgtg cagtatgaaa acgctcgccg gtatccgcgc tggttacgcc gatggcctgg
    21721 tagggcgcgc ggcggacgtc gtgctcaaag aaggccgcaa actggtgctg gtgccgcgtg
    21781 aaatgccgct tagcaccatc catctcgaaa atatgctcgc actttcacgc atgggcgtgg
    21841 cgatggtgcc gccgatgcct gccttttata accatcccga aacggtagat gacattgtcc
    21901 accatgtggt agcccgcgtg ctggatcaat ttggcctcga acatccccac gccaggcgct
    21961 ggcaaggatt gccgcaggcc cggaattttt ctcaggagaa tgaataatgg catttgatga
    22021 tttacgcagc tttttacagg cgcttgatga ccacggccag ttactgaaaa tcagcgaaga
    22081 agtgaacgcc gagccggatc tggcagcagc agctaacgcc accgggcgta tcggcgacgg
    22141 cgcgcccgcg ctgtggtttg ataatattcg cggctttacc gatgcccgcg tggcgatgaa
    22201 caccatcggt tcctggcaga accacgcgat ttccctcggc ctgccgccaa atgccccggt
    22261 taaaaagcag attgatgagt ttatccgccg ctgggataac ttcccgattg ccccggagcg
    22321 ccgcgccaat ccagcctggg cgcagaacac cgttgatggc gacgagatca acctgttcga
    22381 tatcctgccg ctgtttcgtt taaacgatgg cgatggcggt ttctatctcg acaaagcgtg
    22441 cgtggtttcc cgcgatccgc tcgacccgga taacttcggc aagcagaacg tcggcatcta
    22501 ccgcatggaa gtgaagggca agcgtaagct cggcctgcaa ccggtgccga tgcacgatat
    22561 cgtcctgcat ctgcataaag cagaagagcg cggtgaagat ctgccgattg cgatcacgct
    22621 cggtaacgat ccgatcatca cgctgatggg ggccacgccg ctgaaatatg atcagtccga
    22681 gtacgaaatg gcaggcgcgc tgcgtgaaag cccgtacccg atcgccaccg ccccgttgac
    22741 cggttttgat gtgccgtggg gttcagaagt gatcctcgaa ggggtcatcg aaagccgtaa
    22801 acgcgaaatc gaagggccgt tcggtgagtt taccgggcac tactccggcg ggcgtaacat
    22861 gaccgtggtg cgcatcgata aagtctctta ccgcaccagg ccgattttcg aatcgctgta
    22921 cctcggtatg ccgtggaccg aaatcgacta cctgatgggg ccagccacct gcgtgccgct
    22981 gtatcagcag ctgaaagccg agttccctga agtgcaggcg gtaaacgcca tgtacaccca
    23041 tggcctgctg gcgattatct ccaccaaaaa acgctacggc ggctttgccc gcgcggtggg
    23101 cctgcgcgca atgaccacgc cgcatggtct gggctacgtg aagatggtga ttatggtcga
    23161 tgaagacgtt gacccgttca acctgccgca ggtgatgtgg gcgctctcct cgaaagtgaa
    23221 cccggcaggg gatttggtgc agttgccgaa tatgtccgtg ctggaactcg atccaggctc
    23281 aagccctgcg gggatcaccg acaagctgat tatcgacgcc actacgcctg tcgccccgga
    23341 caaccgtggt cactacagcc aaccggtggt ggatttaccg gaaaccaaag cctgggctga
    23401 aaaactgacc gctatgctgg ctgcacgtaa ataaggagaa gaagatgatt tgtccacgtt
    23461 gtgccgatga acagattgaa gtgatggcga aatcgccggt gaaagatgtc tggacggtat
    23521 atcagtgcca gcattgcctt tatacctggc gcgataccga accgctgcgc cgtaccagcc
    23581 gcgaacatta tcccgaagcg ttccgcatga cgcagaaaga tattgatgac gcgccaatgg
    23641 tgccgagcat cccgccgctg ctggtggaag gtaagcgtta aataaaaggt ccgatgccca
    23701 tcggaccttt ttattaaggt caaattaccg cccatacgca ccaggtaatt aagaatccgg
    23761 taaaaccgag aatggtcgtt aacactgtcc aggttttcag accgtctgct acagacaacc
    23821 ccagatattt ggtcacaatc cagaaccctg agtcattaat atgtgacgcg ccaagcccac
    23881 caaagcaggc tgccagcgtc accaatacgc actgaatcgg attcaatccc atcaccgctt
    23941 ctgagagtaa cccgccggtt gtcaggattg ctacggttgc tgacccctgc gatgcacgca
    24001 gagccagtga aataataaat gcggctggta acagaggcag gtcaatcatt tgtagcatat
    24061 tggcaagggc tttgccgacg cccgattcca ccagcacttt gccaaatacc cctccagcac
    24121 cagtaaccaa aatcactacc gccgcagtgg gaagggctga gcccataatg tcgctggtgt
    24181 gttgtaagct ccagccgcga cgtaaagcca ataaccagaa tgccagcacc agcgcaatca
    24241 ttagagctac cattggtgag ccgatcagct gtagcgtacc aagcagggga tgcgaaggcg
    24301 gcatcagtgt tgcggaaacc gtacccgcca tgataatcgc gataggaata acaattagcg
    24361 aggtgaccag cgcgacgccc ggtggattta ttttatcgct taattttgtt gcgccttcct
    24421 cactggccgg agccagttgc atctgttcca gtacttccac cgacatcgca tattggcgcg
    24481 tattgattat tttcgctgca aagtagccaa caatccctac gggaatagaa atcgcaatac
    24541 cgatgatggt tagccagccg atgtctgcgt ggagtaaccc cgctgcggcg acagggcctg
    24601 gatgcggcgg taccgccaca tgaacagtta gcatgattcc agcgacaggc aggccaaatt
    24661 tgagtggcga tattttggca accttggcaa aaccgtaaat gattggcgca agaataataa
    24721 agccgacatc aaagaagacg ggaataccga ggaagaacgc tgccagagtc agcgcagcga
    24781 tagttcgttt gtcacctaac ttgcgactga aataattagc cagtgactct gcaccaccag
    24841 agtgttcgat catacgcccc agcatagcgc ccagaccaat aataatagtg acggaaccaa
    24901 gcacaccgcc catcccggcg atcatcactt tacccacttc gcccgccggt atacccgccg
    24961 caagtgcgac taacaggctg acgaggagca gagcaacgaa tggttgtacc tttgccttga
    25021 tgaccagcag caacagcatg attacgccag ctaacgcaat gcataacaat gtaattgtgg
    25081 acatgggaaa ccctgtctga aagttatagt taacctaccc catccgtaga tggggggatg
    25141 tatgggtacg ttgtaattag ggatttaacg aattagcgcc aggcgtcaaa ccagccaagc
    25201 ccttcttcgg tgaggccacg aggtttatat tcacaaccga tccagccctg atatcccacc
    25261 tcatcgaaca ggcggaacag ccacggatag ttgatttctc catcgtccgg ttcatgtcga
    25321 tcaggtagtc cggcaatttg tacgtgcgca tatttcccag cgtagtcgcg gattaaatgc
    25381 gtcaggttgc catctacttt ttgcgcatga aaagtatcta gttgaataaa cacgttatct
    25441 cgcgcaacct cttcaacaat agccagtgcc tgatactggc tggagaagag ataatgaggc
    25501 ttaacgtcgg ggctgagtgc ttcaactaat attcgcttgc cgtgtagcgc aaagcggtcg
    25561 gcagcgtagc ggagattatc gataaatact gcccggtacc gttcagcgtc ttcgccagcg
    25621 ggcacgacgc ctgccatcac atggacttgt tcacaattga gcgccaatgc atattccagt
    25681 gccaggtcga tgtctgcgtg tgcttcgtgc tcacgtccgg gaagggcgga taatccccat
    25741 tcccccgcat taatatctcc gggagcggta ttgaacagcg ccagtgtcag atggttttgc
    25801 tccagttgct tttggatttg cagggtggag tagtcatagg gaaacagaaa ttccacagca
    25861 tcgaacccgg cttttcgcgc tgcggcgaag cgttcaataa aaggcacttc ggtgaacatc
    25921 atggataaat tagctgcaaa acgaggcatt gcattaactc cttaattccg caatttcacc
    25981 tgcggtcaga taacggatcg ggcggtcacc gagaataaaa atcagctttg ccgtttcctc
    26041 cagctcttcc atattgttgg cggcttcttg caggctttca ccgcaaacca ctgggccatg
    26101 atttgccagt aaaaaagcct gattgtctgc tgccagttcc gccagatcct gtgcgatgcg
    26161 tttatcgccc ggtcggtaat aaggcaccag cgggacattt cccatccgca tcaccacgta
    26221 tggtgtgaac ggacgaataa cgttgctgct gtccagccct tgcaggcagg aaagcgccgt
    26281 cgaccatgtg ctgtgcaaat gcaccaccgc tttacagcgc ggattgttgc gatacagcgc
    26341 cagatgaaag agcacctctt tcgagggttt gtcaccactt aaccattcgc catccgcggc
    26401 gactttggaa agccgctgcg gatcgagatt gcccaggcat gaacctgtcg gtgtcgccag
    26461 taaattcccg tcaggtaaaa gcagcgacag attgccagcc gaaccggttg catagccgcg
    26521 ctgaaagaat gaactggcaa tccgcgtcat ctcctctcgc aaagactgct ctacttttgc
    26581 gaaatcgctc atgataaaaa ctctctttgg gctcgtgaaa aaaaggcttc atcaccgaag
    26641 ttgccagatt taagggcgag tgagacaggc ttatccagtg cgtttaccca cggcacgccg
    26701 ggggaaatgg ttgggccaat atgaaaccct tttatgccca ggctctgtgt gactacgccg
    26761 gaggtctcac cgcctgcgac aataaagcgt gtcacgcctt ccgctgctaa ccgcgccgct
    26821 agttgagaaa atagagtttc tactgcctga ctggcttttt gtgcaccgta ttgctgttga
    26881 attgctgcca atgcgtcagt gctggcggtg gcaaaaacca gtggagcaag tacactttcc
    26941 tggcccagaa cccactctgc cagttcgtgt gcataagcgg ccagagtttc ggttgagagg
    27001 cagcgtgcca catcaacttc acgggctggt gcaatttgac ggtaatgtgc cacctggcgg
    27061 ttggtcattt gagagcatga accggagagc actacgccgc gcccagcgag cggatgccct
    27121 gcttcgcgag cctggttacc gttttcttgc gcccactgcc gggccaggcc aatcgccaga
    27181 ccagaaccgc ccgttaccag tggggcatcg cgcaaggctt ctccctgaat ttccagatgg
    27241 tgttcggtca gcgcatcaag caccgcgtag cggtagccct cttgctgtaa gcgagccagc
    27301 tcttgacgaa cggcatccac accttgttcg aaaacatgtg ccgaaacgac gccgcagcgc
    27361 cctgtggatt gcgcttcaac cagacgggga agatagctgt cggtcatggg atttaccggg
    27421 tgatggcgca tcccggattc ggccagcagt tgattcatta cgaacaaata cccctgataa
    27481 accgtacgtc cgttgaccgg cagggccgga gagaagaccg taaacggcgt gtcgagagca
    27541 tccattaatg catcggtaac cgggccgata ttacctttcg ccgtactgtc gaaagtagag
    27601 cagtatttga aatagatctg tttgcaacct tgctgttgca accagctcag agccgccagc
    27661 gattgctgtg tggcttcaac caccggacag gagcgcgttt tcaggctgat caccagtgcg
    27721 tcgattgctt ccggcatttt acctgttgga acaccgttaa tttgtaccgt tggtagaccg
    27781 ttttccacca gaaaactggc gatatccgtc gcgccggtaa aatcatcggc gataacgcca
    27841 atcttgatca tgatttcgct cccggtagag tgatgccaga gaaaatcttg ataactgcgc
    27901 tatcgtcttc tttcccgtaa cctgcgttac tggcgctggt gaacatattc aatgctgttg
    27961 aggccaatgg cagcgggaag tgcagggctt tggctgtatc ggcaaccaga ccaagatcct
    28021 taacaaaaat atcgacggct gaatgcgggg tgtaatcgcc atccaccaca tgacgcatcc
    28081 ggttttcgaa catccaggaa tttccggcgg cattggtcac gacgtcatac atcacatcca
    28141 gcgggatccc cgcacgggct gcaagtgcca tcgcttcggc tccggcagca atatgtacgc
    28201 ccgctaacaa ctggtgaata atttttacgg tcgaacctag tcccggttct gcacctatgc
    28261 gataaacttt tccggcaacg gcttccagca cgggtgccag tcgttcaaag gcaatatcgc
    28321 taccggaggc catgacagtc atttcaccgt tagcggcttt tactgcacca ccagaaactg
    28381 gcgcatccag catttccaga tcgaatccag ccagagcggt agcaatttct tgcgcatcag
    28441 cactagcgat agtggaagaa accattactg ccgtaccggg tttcagatgt tgtgcaacgc
    28501 ctgtttcacc aaacagcacc tgtttaacct gggccgcatt gaccaccagc accagcagag
    28561 cgtccagttt ttcggcaaac gtcgcggcgt tatcagaaac cccgcaagca cctgcctctt
    28621 tcaacgtagc gcaggcattg ctgttcaggt ctgcgcccca ggtagaaaga cctgcgcgga
    28681 cacatgacag tgctgctccc attcccattg accctaagcc aacgataccg acatgaaact
    28741 cagatcccgt tttcatctgc tctccttgtt aatttaagtg atattttgtt tgatattgtg
    28801 aatataagcg ctggaagata acgatatggt gagctgattc acataaatta acattgtgtg
    28861 ttattttatg tgaactaagc gttagttgcg ccgcgcacgt ttcgcaggca aatagcgtag
    28921 aatgtcagca ggacaaagga aggagcaaaa gttgataccc gtagagcgtc gccaaatcat
    28981 ccttgagatg gtagctgaaa aaggcattgt cagtattgct gaactaacgg acagaatgaa
    29041 tgtgtcacat atgaccattc gtcgggattt acaaaaactg gagcagcagg gagccgttgt
    29101 gctggtgtcc ggaggcgtcc agtctccggg acgcgtggcg catgaacctt ctcatcaggt
    29161 aaaaactgcg ctggcaatga cgcaaaaagc ggctattggc aagctggcgg caagtcttgt
    29221 tcagccggga agttgtatct atctggatgc gggaacgacc acgttagcga tagcacagca
    29281 tctgattcac atggagtcac tgactgtggt cacaaacgat ttcgttattg cgaactactt
    29341 gctcgacaac agtaattgca caattattca cactggcggt gcagtgtgtc gggaaaaccg
    29401 ttcctgtgtc ggggaagccg ctgcgaccat gctgcgcagc ctgatgattg atcaggcttt
    29461 tatttctgca tcgtcatgga gtgtgcgggg gatttctacg ccagcggaag ataaagtcac
    29521 ggtgaaacgg gcgattgcca gtgccagccg ccagcgagtt ttggtctgtg atgcgacgaa
    29581 atatggtcag gtggcgacat ggctggcgtt accattaagc gagtttgatc agattattac
    29641 agacgacggt ttgccggaga gtgccagtcg cgcgctggcg aagcaggatc tctctttgct
    29701 ggtagcgaaa aatgaataat ggcctgcaat aacatctggt tactcatgct tcacagaaga
    29761 agcatgagac taccttattt tataaaatga cagccgccca cttttcggcg atccggtatc
    29821 aatataaatc tggttagcga acgtctgaat gttatcaaac atcatatgtc caaatataaa
    29881 ataatcagcg ccgtttattt gttgtaactc gccattaagc gatttctgca cacgatcaac
    29941 aggccagagt aattcgctct ccgctatttc tttaccaaag agatattcac tcctcggata
    30001 atctgcatgt gcgatgacat attttatgtt gtcgttagtg atttcaataa tatgtggaag
    30061 gtgatggaat ttcagcaaca gatctgtagc ttctttttgc tctgaatcat ttaaatcgaa
    30121 aaaccagtca ccaccgctgg caagccacat attgccatcg ccggtcgcga acgcatccag
    30181 cgccattgct tcgtggttgc ctttaacgga gataaaccag ggctggttta gcaggcgcag
    30241 gacgtcaaga ctctccggcc cacgatcaat attatcgccg acagaaataa gtaagtcggt
    30301 ttcggggaaa aaagagagtt gatgtaagcg ggattgtaat aactgatatt caccatgaat
    30361 atcaccaacg acccatatat ggcgatagtg atgggcattg attttttgat agcgtgtaga
    30421 tggcatggtt ttaccctata aaataagctt tcctattata cagggtattt ttatttgatt
    30481 cgtcagttgt cgttaatatt cccgatagca aaagactatc gggaattttt attacaccag
    30541 actcttcaag cgataaatcc actccagcgc ctgacgcgga gtcagtgaat ccgggtccag
    30601 gttttccagt gcctcgaccg caggcgaagt ttcttctggt acggacagca aagacatttg
    30661 cgtaccatcc acttgcgtag cggcggcgtt cggcgaaatg ctttccagct cacgcagttt
    30721 ttgccgtgcg cgcttaataa cctctttcgg tacgcccgcc agagcagcaa ccgccagacc
    30781 gtagcttttg ctcgccgcgc cgtcctgcac gctgtgcata aaggcgatag tgtcgccgtg
    30841 ctccagcgca tcgagatgta cgttggcgac gccttccatt ttctccggta gctgggtcag
    30901 ctcgaaatag tgggtggcaa acagcgtcaa tgccttaatc ttattcgcca gattttctgc
    30961 gcacgcccac gccagcgaca gaccatcgta ggtggacgtt ccacgcccga tctcatccat
    31021 taacaccaga ctgtattcgg tggcgttatg taaaatattg gcggtttcag tcatctccac
    31081 cataaaggtt gagcgcccgg aagccaggtc atctgccgcg cctacgcggg taaagatgcg
    31141 atcgataggt ccaatctcga ctttttgtgc cggtacatag ctgccgatgt aggccatcag
    31201 cgcaatcagt gcggtctggc gcatataggt acttttaccg cccatgttcg ggccggtaat
    31261 aatcaacata cggcgctgcg gcgacagatt cagcgggtta gcgataaatg gctcattcag
    31321 tacttgttca actaccggat ggcgaccttc ggtaatgcga atgcccggtt tatcaatgaa
    31381 ggtcgggcag gtgtagttca gggtataggc ccgttccgcc aggttaacca gcacgtcgag
    31441 ttccgccagc gcgctcgcgc tctgttgcaa cgcttccaga tgcggcaaca gcaggtcgaa
    31501 cagctcttca taaagctgtt tttccagtgc cagtgctttg ccttttgagg tgagaacttt
    31561 atcttcgtac tcttttagct ctggaatgat gtagcgctcg gcgtttttca gcgtctggcg
    31621 acgcatgtag ttgatgggtg ccagatggct ttgcccacgg ctgatttgaa tgtagtagcc
    31681 gtgcaccgca ttaaagccaa ctttcagcgt gtccaggccg gtacgttcac gctcgcggac
    31741 ttccagacgc tccagataat cggtcgcgcc gtcagccagc gcgcgccact catccagctc
    31801 ttcgttatag cccgatgcga taacaccacc gtcgcgtacc agcaccggcg gtgtgtcgat
    31861 gattgctcgc tccagcagat cgcgcagctc ggcaaactcg cccatcttct cacgtagcgc
    31921 ctgtaccggt gcactatcga cagtttctaa ctgcgcacgc agctccggca gttgctggaa
    31981 agcgtggcgc atacgggcca gatcgcgtgg gcgagcagtt cgtaaagcca gacgtgccag
    32041 aatacgttcc aggtcgccga cctgacgcag taccggctgc aactcggcgg tgaaatcctg
    32101 caatgcgcca atagtttgct ggcgctcaag caacacgcgg gtatcgcgca ctggcatatg
    32161 cagccagcgt ttcagcatac gactacccat cggcgtgacg gtgcagtcga gcacagaagc
    32221 cagcgtattt tccgcaccgc ccgccaggtt ctgagtaatt tccaggttac gacgtgtcgc
    32281 ggcatccata atgatgctgt cctgctcacg ttccatggtg atagaacgaa tatgcggcag
    32341 ggtcgtgcgt tgggtatctt tcgcatactg caacagacaa ccggcagcac aaagtccgcg
    32401 cggcgcgttc tcgacgccaa aaccgaccag atcgcgggtg ccaaattgca gattcaactg
    32461 ctggcgcgcg gtgtcgattt caaactccca cagcgggcga cggcgcaggc cgcgacggcc
    32521 ttcaatcagc gacatctcgg cgaaatcttc tgcatacagc agttccgccg gattagtgcg
    32581 ttgcagctct gccgccatcg tttcgcggtc ggccggttcg ctcaggcgaa aacgcccgga
    32641 gctgatatcc agcgtcgcgt agccgaaacc tttgctgtcc tgccagatag ccgccagcag
    32701 gttgtcctga cgctcctgta acagggcttc atcgctgatg gtgcctggcg taacgatacg
    32761 cacaactttg cgctcaaccg gacctttgct ggtcgccgga tcgccaattt gttcgcagat
    32821 ggcaacggac tctccctgat tcaccagttt ggcgagatag ttttccaccg catggtaggg
    32881 aatccccgcc atcgggatcg gctctcccgc cgaagcaccg cgtttggtca gtgaaatatc
    32941 cagcagttgc gacgcgcgtt ttgcgtcgtc ataaaacagt tcataaaaat cacccatccg
    33001 gtaaaacagc aggatctcgg gatgctgggc tttcagcctg agatactgct gcatcatggg
    33061 cgtatgggcg tcgaaatttt ctattgcact catggggtta tgtccggttc cctgatatta
    33121 aatggggtgt gatggttttt attttcgttg taatcacata aggcgcacat gataccggag
    33181 ttaatcattt caggggaaga agcgaacggt gagttcttga tgctggttcc gggaaagatg
    33241 ctgttttgca gcttttgcgt gatgcttatg aagcaagtac gcaaaattgc gtcggttggc
    33301 gaacctttct tctttagagt catagtgccc tttaagatta agatgtaata taaatgcatc
    33361 ttaatgaggt ggaggcagca tgtccggcaa gcgtatctct cgtgaaaaac tgacgattaa
    33421 aaaaatgatc gatctttatc aagcgaaatg cccgcaggcg tcagcggagc cggagcatta
    33481 cgaggcgttg tttgtttacg cgcaaaagcg gctggataaa tgtgtgttcg gcgaagagaa
    33541 accagcctgt aagcagtgtc cggtgcactg ttaccagcct gcaaagcgtg aagagatgaa
    33601 acagattatg cgttgggccg ggccgagaat gctgtggcgt catccgatct taaccgtgcg
    33661 tcatctcatc gatgacaagc gtccggtgcc agaactgccg gaaaaatatc gtccgaaaaa
    33721 acctcgtgaa tagtatgccg gacaggcaga tctgtccggc aatttgcagt taaatcaatg
    33781 ccgatttatc aattcccagc cgcttcatcc gtgacagcag ggtcgtgcgt ttcagcccca
    33841 gacgttgcgc agcgccttta ggcccggcaa ccacgccgtt ggtttctttc agcacgcgaa
    33901 caatcaactg atattcatct tcgccctcct gggcgacaac cgttgcggca ggcggcgttt
    33961 caggttccgg taaaacaata tctggcaatg acagctgcag cacgttaccg cgtgttagca
    34021 ataccgcgcg ctcaatgacg ttttccagtt cgcgtacgtt acccggccac tccatgttgc
    34081 tcaaggtgcg cagcgtctcg gcaggaatgc tgtcgatatt gcgccccaga cgacgggcaa
    34141 ttttgaaggt aaaggctttc gccagcagcg gaatatcttc cggacgctcg cgtagtggcg
    34201 gcaggtgaat cgggaatacg ttcaggcggt aatagagatc gctacggaac tcacggtcgg
    34261 cgaccatttt tttcagatcg cggttagtcg cggcgattag acgcacgtcc gtctgaatga
    34321 ttttgttgct gccgagacgt tcaaactcct gttcctgcaa tgcacgcagc aacttcggct
    34381 gtaactccag tggcatatcg cccacttcgt cgaggaacag ggagctttta tccgccagtt
    34441 caaaacgacc aatgcgctgg gcgctggcac cggtaaaagc cccacgctca tgaccaaaca
    34501 gatcgctttc cagcaatccg gcaggcatcg ccgcgcagtt cattttgacc atgcggcgat
    34561 tattacgccc actgagatta tggatcgcac gggcaatcag ctctttaccc gtgccagttt
    34621 caccgaggat cagcacggta ctgtcacttt gcgccaccat ttcaacttgt ttaagcacgc
    34681 tgtacatggc ttcgctgcgg ccaataatct cgccaaattc actatcaaca ttgttgagct
    34741 gctcggtcag ggcgaggttt tcatcaacca gccgttcttt cagacgatgg atttcctgat
    34801 aggcgagggc gttatcgaca gcgattgcca cacgttcggc aatctggcgc agtaaattca
    34861 gattggtagt ggtaaacact ttctcttcgc attgcgccag tttcagcacg cccagcatgg
    34921 tgtcgccaga catcagcggt aacaggcaca aggtttgaat ctggttgccc caggtgtcga
    34981 acaacatgcg ttcatagggg gctaaatcgt cccgctcgtg gagattgatc agcagcatct
    35041 ctttactttt gaacacgcgt tcggtgaggg ttccggcttc atcgacttcg ctctgttcgt
    35101 gggcgggatg ttgtttatca agatagtgag tggagtagat gttgagtttg tttttacggt
    35161 ggctgcgtaa gacgatactg atatcgtcaa tgtcgaaata gtaatggatt tctttggcga
    35221 cttcgctgac cagttcgtcc atatccaggc gggaaagcac cgcgttggta atggcaacca
    35281 ggatgcggaa gttatcgcgc tcccggcata acaactcata gtcgacattg ttgacgacgc
    35341 ggctctggat ttgttcggtg acgacagaaa cgatctgcgt aaatgtttgc agacgattga
    35401 actctttttc gctccagggg cgatcgtcat aacgaataaa ttcacagcca ccaaaaatat
    35461 gcccttctgc cgccagtggc atcaggcaat agtgaccaaa ttttgggtat agcccacctg
    35521 cggccagctg cggccaggtt tcacaaaatt cttcgtaact gcaatgcagc gtatcagggc
    35581 gcgacaaaat gctgcgtacc ggaccgtgtg ccagaacagt ttcgtcttca tatttaatgg
    35641 gggtgtcttt ttcacgcgat gcgtaataag acgcacgttg agtctgcgct tgccacaaca
    35701 caatagccgc gttgtcggcg agcgcagaac gctttaccag ttgcgaaaga gcctcacaca
    35761 gcgaggccag atcgggctgc tgcaatagtg tccgagtgat gtcgaacaac ccttgctgtc
    35821 cgagatcact catcggtgta tatgacattt tgttcttcca ggaaagcacc gcaaagcggt
    35881 gcaaaaggtc attgactaac tataggagat ttagaatttt attagcatat acgcggaagc
    35941 ggttcggcgt gtggtaaatc gagggttcgt ttcacgccat acagaccggc aagacgaaca
    36001 cctttacgtt ccaccacttc accaatcagc gccgcgtctt tccccagtgg atgggaatgt
    36061 aacgctgcca gcacttgctc tgccgcgttg cgttcaacgg cgatcaccag tttgccttcg
    36121 ttggcaaagt tcagggcgtc cagtcccagc aattcgcaaa cgccgcgcac ggcaggttta
    36181 accggcagcg ctgattcaga aatttcaata ccgcaaccgc aggctgccgc gaactcatga
    36241 accaccgcat ttacaccacc acgggtggca tcacgcagcg ctttcacgcc gggaatgtca
    36301 cgcagcgtct gaataagcgg cgtcagcacc gcgcagtcgc tgaccagttc gccatccagc
    36361 cccagctgct cacgcaggtt aaggatagtc gccccgtggt cgccgagcgt gccgctcacc
    36421 agcaacacat cgcctgcggt tagcgtctgt gcgccccaat gaatattcgc cggaattgcg
    36481 cccatgccag cggtgttgat aaacagttta tctgccgcgc cgcgctgcac cactttagta
    36541 tcgccagtaa cgatggcaat gcctgccgtg cgcgcggttt ctgccatgct ggtcactacg
    36601 gctttcagtg tctccatcgg caatccttct tcgaggataa agccacagga gagatagcgc
    36661 ggaatagcgc cactgaccgc aacgtcattc gcagtgccgc aaatcgccag cttgccgata
    36721 ttaccgccag gaaagaacag cgggtcaatg acgtaactgt cggtggagaa cgccagacgg
    36781 tcgccttccg ctaccagctg cgccagatca agacgtgcct gatcttcctg ctctgccagc
    36841 cacgggttgg caaaggcttc cataaacagg ctgttgatta attgctgcat cgcctggccg
    36901 ccgctgccgt gggcgagttg gatattattc acgcttcact ctcctgctga cgatactgat
    36961 accacgcggc gcacgctcct tcggaggaga ccatcagtgc accaaatgcg gtttgaggat
    37021 tacaggtgtt accaaacagc gggcattgat gcggcttaca tttgcccgtt aatacttcac
    37081 cacaacgtgc gcgcgggtca tcgcagacct gctgcggtgc cgggcggaaa tgtgcttcgg
    37141 catcgaatcg ttgataatcc ggcgtcaggt gtacgccaga agattcaatc acgcctaagc
    37201 cgcgccattc gctgtcgccg ttgacacaga acacatcggc aatcgcctgt tgcgccagca
    37261 ggttaccggc atccggtacc actcgacgat actgattctc taccttgctg tgggccgcta
    37321 ttttctgctc caccagcatg accacgcctt gtagtagatc aaggggttcg aaaccagcga
    37381 ccaccagcgg acgatgaaaa tcgctggcga taaaattata ggcatcagtg ccgataacca
    37441 tactaacgtg gcccggcgcg aggaacgcgt cgataccgtt atccggctgt tccagcaaac
    37501 tgcgcagcgt cgggataagc gtaatatgct ggcagaagaa gtaaaaattc tgcacatcgc
    37561 gggctttcgc ctgttgcagg gtgatggcgg tggtcggcat ggtggtttca aaacctaagc
    37621 cgaagaatac gactttacgg gttggattct cctgcgccag tttcaacgca tccatcggcg
    37681 agtaaacgat gcgcacatcg gcaccgcgtg cttttgcctg caacagcgat ccctgtttcc
    37741 ctggtacgcg catggcgtcg ccaaaggtac agaagatgac ttccggatgg ctggcaatct
    37801 ccacgcaggt gtcgattcta cccatcggca gtacacacac cggacaaccc ggaccgtgga
    37861 taaactcaac gttttccggc agtaactggt cgaggccgaa tttaaagata gcgtgggtat
    37921 gaccgccaca cacttccata atccgcagag ggcgttcggc ggtgtaagag agatgtgaag
    37981 cacgttcgcg cagatgctca attaactgca tcacctgttc cggcgcgcga tattcatcaa
    38041 caaaacgcat tatttttcct cgccatacaa cagcgcgccg acatccggct caacgtcaaa
    38101 catgttttgt aaggcgtcga gagtgtcgcg tgcttcggct tcattaatta cgctcatggc
    38161 aaagccaacg tgaaccagta cccactggcc cacgcgcggc tgaccgtttt catcgcagct
    38221 gccgactaac gttaaatcga catcgcgctg aatgccgcag acgtcgactt tcgcctggtt
    38281 gccgtcaatg gtgcggatct ggccgggaac gcctatgcac atcgctgtgt ctccagccag
    38341 ttcagccact ggtccatccc ttcgccgctg gtggcggaaa taaggatgat ttcaatttct
    38401 ggattgactt cgcgtgcgca ggcgatgcac ttctcaacgt caaagttgag atacggcaaa
    38461 aggtcaactt tgttgagcag catcagcgag gcggcggcaa acatatgcgg atatttcagc
    38521 ggtttgtctt caccttcggt aacggaaagg accgctactt tgtgtttttc accgagatcg
    38581 aagctggccg ggcatacgag gttgccaacg ttttcgataa acagaatacc gttatcgtcc
    38641 agtggcagac gcggtgcggc gtcggcaatc atctgtgcgt caaggtggca gcctttacca
    38701 gtgttcacct gaatcgctgg tgtgccggta gcgcgaatgc gtgcggcatc gttcacggtt
    38761 tgctgatcgc cttcaataac tgcgcacgga acgctgtctt tcaggcgcat taaggtttcc
    38821 gtcagcaggg tggttttacc ggaaccaggg ctggaaacca ggttgagcac cagttgcttg
    38881 cgggcagcaa agcgcgcgcg gttgcgttca gccagacggt tatttttgtc cagcacgtca
    38941 atttcgactt ccagcatccg acgctggctc atgcccggcg cgtgagtgcc cgcttcgcca
    39001 tgaccgtaat gcaggtcgcc ttcttcagtc tggctggggg taaattcagg cgctttaatg
    39061 ccggtgattt tcatcttcgg gcgtgccgcc ggggcaaatg gcgcgctacg aaacgcggaa
    39121 tgagggttat gttcatcacc ctcgatatac aggttgcctt caccgcaacc gcatgttgta
    39181 cacatcgctc actcctggtc tatttctatc cgccgaatct gtaaaccgtc gtctgccaca
    39241 atctgtagca tgtcaccatg acactgtgga cagcggcgga cgcgctgggt cagtagcgtc
    39301 acatactgtt ggcatgtttc acaccagcat tcggcctctt gttcttcgag gtgcagttta
    39361 caaccttccg ccacgctgcc gcggcaaacc agatcaaaac aaaaggcaag agagctggtt
    39421 tcgacacaag aaaatgcgcc aattttgagc cagaccccag ttacgcgttt tgcgccgtgt
    39481 tttgcggcct gctgttcgat caattccagt gcccgttggc agagggttat ttcgtgcata
    39541 tcgcctccca ttaactattg ccagctacaa gcaataattg tgccagtgtt gattatccct
    39601 gcggtgaata atgtcgatga tgtcgaaatg acacgtcgac acgtcgacac ggcgacgaaa
    39661 ttcatcttta gcttaaaaat ctctttaata acaataaatt gaaagttggc acaaaaaatg
    39721 cttaaagctg gcatctctgt taaacgggta acctgacaat gactatttgg gaaataagcg
    39781 agaaagccga ttacatcgca cagcggcatc gtcgcctaca ggaccagtgg cacatctact
    39841 gcaattcgct ggttcagggg atcacgttat cgaaagcgcg cctgcatcac gccatgagct
    39901 gcgcgccgga caaagaactc tgtttcgtcc tttttgaaca ttttcgtatt tacgtcaccc
    39961 tggcggatgg ctttaacagc cacaccatcg agtattacgt cgaaacaaaa gatggcgaag
    40021 acaaacagcg gattgcgcag gcgcaactga gcattgacgg catgattgat ggcaaggtca
    40081 acatccgcga tcgcgaacag gttctggaac actatctcga aaaaatcgct ggcgtttacg
    40141 acagcttata caccgccatt gaaaacaatg tgccggtgaa tttaagccaa ctggtaaagg
    40201 gacaaagccc ggcagcatga gctgaggctt tgcccgtttt gcaggcgtta cgcctgtttg
    40261 gggatgggcg tgtcgatgag tgtcgaaaat gtcatttcat cggcatgttt tcgtcaaaaa
    40321 tgacaatcac ctgaggaatg cctggtgaat cgttttgtaa ttgctgactc cacgctctgt
    40381 atcggctgcc acacttgtga ggccgcctgt tcagagacgc atcgccagca cggcctgcaa
    40441 tcaatgccgc gcctgagagt gatgctaaat gaaaaagaat ctgcgccgca gctctgtcac
    40501 cactgtgaag atgcaccctg cgcggtggtc tgcccggtta acgccatcac ccgcgttgat
    40561 ggggccgtgc agttgaatga aagcctgtgc gtaagctgca aactgtgcgg catcgcctgc
    40621 ccgtttggcg caattgaatt ttccggcagc cgtccgctgg atattccggc aaacgccaat
    40681 accccgaaag cgccaccggc accgcctgct ccggcgcgta tcagtacgct gcttgactgg
    40741 gtgccgggta ttcgcgcgat cgccgtcaaa tgtgaccttt gtagcgttga tgaacaaggt
    40801 ccggcctgcg tgcggatgtg cccgaccaaa gccctgcatc tggtggataa caccgatatc
    40861 gcccgcgtca gcaaacgtaa gcgtgagctg acctttaaca cggactttgg cgatctcacc
    40921 ttgtttcagc aggctcaaag tggagaggct aaatgagcgc aatttccctg atcaatagcg
    40981 gcgtggcgtg gtttgtcgcc gccgctgttc tggcatttct cttttctttt caaaaagcgc
    41041 taagtggctg gatagccgga attggcggcg cggttggtag cctgtatacg gcagccgcgg
    41101 gcttcactgt actgactggc gcggttggcg tgagcggtgc gctgtcgctg gtaagctacg
    41161 atgtgcaaat ctctccgctt aacgcgattt ggctgattac gctcggtctg tgcggtctgt
    41221 ttgtcagcct ctataacatt gactggcatc gccacgcgca ggtgaagtgc aacggcttgc
    41281 agatcaatat gttgatggct gccgccgtct gcgccgtcat cgccagcaac ctcggcatgt
    41341 tcgtggtaat ggctgaagtc atggccttgt gtgcggtgtt ccttaccagc aacagcaaag
    41401 agggcaaact gtggtttgcg ctggggcgtc ttggcactct gctgctggtg attgcttgct
    41461 ggctgctgtg gcagcgttat ggcacgctgg atctgcgcct gctggatatg cgtatgcaac
    41521 agctgccgct cggttccgat atctggctgc tcggtgtgat tggctttggc ctgctggccg
    41581 ggattattcc gctgcacggc tgggtgccgc aggcacatgc taacgcctct gcgccagctg
    41641 ccgcactgtt ttccacggta gtcatgaaaa ttggcctgct gggcatttta accctgtcac
    41701 tgctgggcgg taatgcaccg ctgtggtggg ggatcgcgct gctggtgctc ggcatgatca
    41761 ccgcgtttgt cggtggtctg tatgcgctga tggagcacaa catccagcgc ctgctggctt
    41821 accacaccct ggaaaatatc ggcatcatcc tgctggggct gggcgctggc gtaacgggta
    41881 tcgcgctcga acaaccggcg ctgattgccc ttggtctggt cggtggtctg taccatctgc
    41941 ttaaccatag cctgttcaaa agcgtactgt tcctcggggc ggggagcgtc tggttccgta
    42001 ccggtcatcg cgatatcgaa aaactcggtg gtattggcaa gaaaatgccg gttatctcca
    42061 tcgccatgtt agtcgggctg atggcaatgg ctgcgctgcc gccgctgaac gggtttgccg
    42121 gggaatgggt tatctatcaa tcctttttca aactgagcaa tagtggtgcg tttgttaccc
    42181 gtctgctggg accgctgctc gctgtgggtc tggcaattac cggtgcgctg gcggtgatgt
    42241 gtatggcgaa agtctatggc gtcacgttcc tcggcgcgcc gcgtaccaaa gaagccgaaa
    42301 acgccacctg tgcgccgctc ctgatgagcg taagcgtagt ggcactggcg atttgctgcg
    42361 taattggcgg tgttgctgcg ccgtggctgc tgccgatgct ctctgctgct gtacctctgc
    42421 cgctggagcc agctaacacc accgtttctc aaccaatgat cacattgctg ctgattgcct
    42481 gcccgctgct gccattcatc attatggcga tttgcaaagg cgatcgtttg ccgtcgcgtt
    42541 cccgcggtgc ggcctgggtg tgcggctacg accacgaaaa atcaatggtg attaccgctc
    42601 acggttttgc catgccggtg aaacaggcgt ttgcgccggt gctgaaacta cgcaaatggc
    42661 tgaatccggt gtctctggtg ccgggctggc agtgcgaggg gagtgcgttg ctgttccgcc
    42721 ggatggcgct ggttgaactg gcggtactgg tggtgattat tgtttcacga ggagcctgag
    42781 aatgagtgtt ttatatccgt taattcaggc gctggtgtta tttgccgttg cgccgctgct
    42841 ctccggtatt acccgcgtgg cgcgcgcccg cttgcataac cgtcgcgggc cgggcgtgtt
    42901 gcaggagtat cgcgacatta tcaaactgct gggtcgccag agcgttggcc cggatgcctc
    42961 cggctgggta ttccgcctga cgccgtatgt gatggtgggc gtaatgctga ctatcgctac
    43021 tgcgctgccg gtggtgaccg ttggttctcc gctgtcgcaa ctgggtgatt tgatcacctt
    43081 actgtatctc tttgccatcg cgcgtttctt ctttgccatt tctggtctgg ataccggtag
    43141 cccgtttacc gctatcggcg cgagccgtga agcgatgctg ggtgtgctgg tcgaaccgat
    43201 gttgctgctt ggtctgtggg ttgccgcaca ggtttccggt tccaccaaca tcagcaacat
    43261 caccgacacc gtttatcact ggccgctgag ccagagcatc ccgctggtgc tggcactttg
    43321 tgcctgtgca ttcgctacct ttatcgaaat gggcaaactg ccgttcgacc tggcggaagc
    43381 cgagcaggag ttgcaggaag gtccgctctc tgagtacagc ggcagcggct ttggcgtcat
    43441 gaaatggggt atcagcctga aacagctggt ggtgctgcag atgttcgtcg gggtgtttat
    43501 tccgtgggga caaatggaaa ccttcaccgt cggtgggctg ctgctggcgc tggtgattgc
    43561 catcgtcaaa ctggtggtcg gcgtcctggt tatcgcgctg ttcgaaaaca gcatggcccg
    43621 tctgcgtctt gatattactc cgcgcattac ctgggctggg tttggctttg catttttagc
    43681 gttcgtctcc ttgctggcgg cgtgattaaa gagagtttga gcatgtctga agaaaaatta
    43741 ggtcaacatt atctcgccgc gctgaatgag gcatttccgg gcgtcgtgct ggaccacgcc
    43801 tggcagacca aagatcagct gactgtcacc gtaaaggtga actatctgcc ggaagtggtg
    43861 gagtttctct actacaagca gggtggctgg ctgtcggtgc tgtttggtaa cgacgaacgc
    43921 aaactgaatg gtcattacgc cgtttattac gtgctgtcga tggagaaggg cactaagtgt
    43981 tgggtaacgg ttcgcgtcga agttgacgcc aacaaaccgg aatatccgtc cgtgacgccg
    44041 cgcgttccgg cggcggtgtg gggcgagcgc gaagtgcgcg atatgtacgg tttgattccg
    44101 gttggtctgc cggatgaacg tcgtctggtg ctgccggatg actggccgga tgaactttat
    44161 ccgctgcgaa aagacagcat ggattatcgt cagcgtccgg caccgaccac cgatgctgaa
    44221 acctacgagt tcatcaacga actgggcgac aagaaaaaca acgtcgtgcc gattggtccg
    44281 ctgcatgtca cttctgacga accgggtcac ttccgtctgt tcgtcgatgg cgaaaacatt
    44341 atcgacgccg actaccgcct gttctacgtc catcgcggca tggaaaaact ggcggaaacc
    44401 cgtatgggtt acaacgaagt gaccttcctc tctgaccgtg tgtgcggcat ctgcggcttt
    44461 gctcacagca ccgcctacac cacgtcggtg gaaaacgcga tgggtattca ggtgccagaa
    44521 cgtgcgcaga tgatccgcgc cattctgctg gaggtggaac gcctgcactc gcatctgctc
    44581 aacctcggcc tcgcctgtca ctttaccggc ttcgactccg gctttatgca gttcttccgc
    44641 gtgcgtgaaa cctccatgaa aatggcggag atccttaccg gtgcgcgtaa aacctacggc
    44701 ctgaacctga tcggcgggat tcgtcgcgat ctgctgaaag atgacatgat ccagacccgc
    44761 cagctggcgc aacagatgcg tcgtgaagtg caggagctgg tggatgtgct gttgagcaca
    44821 ccgaacatgg aacagcgcac tgttggcatt ggtcgtcttg acccggaaat tgcccgcgac
    44881 ttcagtaacg ttggcccgat ggtccgtgcc agcggtcacg ctcgcgatac ccgcgccgat
    44941 cacccgtttg ttggttatgg cctgctgcca atggaagtcc acagcgagca gggctgcgac
    45001 gttatttccc gtctgaaagt gcgaatcaac gaggtctata ccgcgctgaa catgatcgac
    45061 tacggtctgg ataacctgcc gggcggtccg ttgatggtgg aaggctttac ctacattccg
    45121 caccgtttcg cgctgggctt tgccgaagcg ccgcgcggcg acgatatcca ctggagcatg
    45181 accggcgaca accagaagct gtaccgctgg cgctgccgtg ccgcgaccta cgcgaactgg
    45241 ccgaccctgc gctacatgct gcgtggcaac accgtttctg atgcgccgct gattatcggt
    45301 agcctggacc cttgctactc ctgtaccgac cgcatgacgg tggtcgatgt acgtaagaag
    45361 aagagcaaag tggtgccgta caaagaactc gagcgttaca gcattgagcg taaaaactcg
    45421 ccgctgaaat aaggaatcgc catgtttacc tttatcaaaa aagtcatcaa aaccggcacg
    45481 gcgacctcgt cttatccgct ggagccgatt gcggttgata aaaacttccg tggtaagcca
    45541 gagcagaacc cgcagcagtg catcggctgc gcggcctgcg tcaatgcctg cccgtcaaac
    45601 gctttaacgg ttgaaaccga cctcgccaca ggagagcttg cgtgggagtt taatctcggg
    45661 cgctgcatct tctgtggacg ctgcgaagaa gtttgcccga cggcggcgat caaactgtcg
    45721 caagagtacg aactggcggt gtggaagaaa gaagatttcc tgcaacagtc ccgttttgtg
    45781 ctgtgcagct gtcgcgtctg caatcgtcct ttcgccgtcc agaaagagat cgactacgcc
    45841 attgcgctgc ttaagcacaa tggtgacagc cgcgcggaaa accaccgcga aagctttgag
    45901 acttgcccgg aatgtaagcg ccagaaatgc ctggtgccgt ccgaccgtat tgaactgact
    45961 cgccatatga aagaggccat ctgatgagca atttattagg cccccgtgac gccaacggca
    46021 ttccggtccc gatgacggtg gatgaatcca tcgccagcat gaaggcgtcg ttactgaaaa
    46081 aaatcaaacg ttctgcctac gtttaccgcg tggactgcgg cggctgcaac ggctgcgaaa
    46141 tcgaaatttt cgccacgctt tcgccgctgt ttgatgcaga acgcttcggc attaaagtcg
    46201 ttccttcacc gcgtcatgcg gatattttac tgtttaccgg cgcggtcacc cgtgcaatgc
    46261 gatcccctgc gctgcgtgcg tggcagtccg cgccggaccc gaaaatctgt atctcctacg
    46321 gtgcctgcgg taacagtggc gggatcttcc acgatctcta ctgcgtgtgg ggcggtacgg
    46381 ataaaatcgt cccggtagat gtctacatcc ctggttgccc gccaacgcct gccgccacgc
    46441 tgtatggctt tgcaatggcg ctcggcctgc tggagcagaa aattcacgcc cgtggcccgg
    46501 gtgagcagga tgaacaaccg gcggaaatcc tgcatggcga tatggtgcag ccgctgcgtg
    46561 tgaaagtgga tcgcgaagca cgccgcctgg caggttatcg ttacggtcgt cagattgccg
    46621 atgacttcct gacacagtta gggcagggcg aagaacaggt tgcacgctgg ctggaagcgg
    46681 aaaacgatcc gcgtctgaac gagattgtca gccatctgaa tcatgttgta gaagaggcgc
    46741 gtatccgatg agtgaaaagg tggtgttcag tcaactgagc cgtaaattta ttgatgagaa
    46801 cgatgccacg cccgccgagg cgcagcaggt ggtctattac agcctggcga ttggtcacca
    46861 ccttggggtt atcgattgcc tggaagcggc gctcacctgc ccgtgggatg aatatctggc
    46921 atggattgcc actctggagg cgggcagcga agcccgccgc aaaatggaag gcgtgccgaa
    46981 atatggtgag atcgtcatcg acattaacca tgtgccgatg ctggccaacg cattcgataa
    47041 agcccgggca gcgcaaactt cgcagcagca ggaatggagt acaatgctgc taagtatgct
    47101 gcatgatatt catcaggaaa acgccatcta tttgatggtg aggagactgc gtgactgacg
    47161 ttttactctg tgttggcaat agcatgatgg gcgatgatgg cgcaggcccg ctgctggcgg
    47221 aaaagtgcgc cgccgcgccg aaaggtaact gggtggtgat tgacggcggt agtgcaccgg
    47281 aaaacgacat cgtcgccatc cgtgaactgc gccctacgcg actgctgatt gtcgacgcca
    47341 ctgatatggg gctaaacccc ggcgagatcc gcatcatcga cccggatgat atcgccgaga
    47401 tgtttatgat gactacccat aacatgccgt tgaactacct tatcgaccag ttgaaagaag
    47461 atattggcga agtgattttc ctcggcatcc agccggatat cgtcggcttt tactacccga
    47521 tgacccagcc gattaaagat gcggttgaaa ccgtttatca acgactggaa ggctgggaag
    47581 gaaatggtgg atttgcgcag ttagcggtgg aagtagagta gtttttcatt aaggaattca
    47641 gggaattgaa catcagaaca gggatgtgcg cttttttatt gagcttagtg ctgcctgcac
    47701 aggccaccag ctttaccgaa tatctgccga tgagtgacag cgaatacgcg caaaagcggg
    47761 cgctgaaacc tctcctgacc atgccttatg atgccgagca aaactggcat tttagaaaag
    47821 tcggcgttgc tggcgtgacg cttgaaaaga tgccaaatga tgatagtgaa tggcaattga
    47881 acggtaaaga tcgcgccggt aaatcctggt ctgtgcctgt gggggtgctg caaaatatgg
    47941 cgggtaatgc gcaactttac cgtgccgatc ttgatcgtaa cggcattcag gatctggtta
    48001 tctggcgggg tatttcagga aatggtctgg cacctaacgc gtttttgatt ctgatgacct
    48061 ttaatcagca aggtcgcccg tgtgtttttc agtccgatgg tttttatacc gcaagtgaaa
    48121 ctggcattga tgatttgctg gatttacaaa ggaatggcca cacgcaattg ctggatatgc
    48181 agtttgattc cggttactgg ataaccagtc tgtatcaggt aaaagacgcc aaatggcagc
    48241 gtgttcacgg ctggtttggc aagttaagct atcccgcgct cacgcgtttt acttataccc
    48301 ccaaccgtaa actcgtcctc aaacccatcg ccgggcgcga cccgcaaacg gaagatctgg
    48361 cgcagacgca gcgttgcttg ataaaggggg atgtgcttta tggggtgaac cagaactgac
    48421 tttttgcaga gtggctatga gggggattac gtgcagacag acgtcacgtt actacagaga
    48481 gaacaaagaa tgactgctaa tgctgcacgc gctgttaaag caacaagaga actggtcaat
    48541 gcagtcccct ttctgggagg cagtgactca gaagatgatt atcgtgaagc actggaactg
    48601 gtcgaatatt tgattgaaga agatgacacc aatccactta tcgactttct ggcgagccgc
    48661 attgcagagt atgaaaacaa caacgaaaag tttgcagagt tcgacaaagc cgttgcggca
    48721 atgccggttg gcgtggcgtt acttcgcacg ttgattgatc aacataatct gacctatgcg
    48781 gatttgaaga acgaaatcgg ctctaaatcc ctggtcagtc agattttgtc tggacaacga
    48841 tccctgacaa tatcccacat taaggcactt tcagcccgat ttggcgttaa gccggaatgg
    48901 tttctttgat tttgactgat gccaaacccc agattgcccg atgcgctgcg cttatc
//
LOCUS       DAZGSP010000025        48416 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZGSP010000025 DAZGSP010000000
VERSION     DAZGSP010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.025"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(314..389)
                     /locus_tag="ACHK74_001592"
     tRNA            complement(314..389)
                     /locus_tag="ACHK74_001592"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(354..356),aa:Asn,seq:gtt)
     gene            complement(490..1977)
                     /gene="yeeO"
                     /locus_tag="ACHK74_001593"
     CDS             complement(490..1977)
                     /gene="yeeO"
                     /locus_tag="ACHK74_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFP0220437.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPAVSE"
     gene            2138..2213
                     /locus_tag="ACHK74_001594"
     tRNA            2138..2213
                     /locus_tag="ACHK74_001594"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2171..2173,aa:Asn,seq:gtt)
     gene            complement(2251..3201)
                     /gene="cbl"
                     /locus_tag="ACHK74_001595"
     CDS             complement(2251..3201)
                     /gene="cbl"
                     /locus_tag="ACHK74_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFP0220438.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRRGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3303..4220)
                     /gene="nac"
                     /locus_tag="ACHK74_001596"
     CDS             complement(3303..4220)
                     /gene="nac"
                     /locus_tag="ACHK74_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFP0220439.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCLGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4547..4622
                     /locus_tag="ACHK74_001597"
     tRNA            4547..4622
                     /locus_tag="ACHK74_001597"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4580..4582,aa:Asn,seq:gtt)
     gene            complement(4678..5613)
                     /gene="ldtA"
                     /locus_tag="ACHK74_001598"
     CDS             complement(4678..5613)
                     /gene="ldtA"
                     /locus_tag="ACHK74_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP0220440.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGMRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5675..6754)
                     /gene="cobT"
                     /locus_tag="ACHK74_001599"
     CDS             complement(5675..6754)
                     /gene="cobT"
                     /locus_tag="ACHK74_001599"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFP0220441.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6766..7509)
                     /gene="cobS"
                     /locus_tag="ACHK74_001600"
     CDS             complement(6766..7509)
                     /gene="cobS"
                     /locus_tag="ACHK74_001600"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFP0220442.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7506..8051)
                     /gene="cobU"
                     /locus_tag="ACHK74_001601"
     CDS             complement(7506..8051)
                     /gene="cobU"
                     /locus_tag="ACHK74_001601"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFP0220443.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(8185..8622)
                     /locus_tag="ACHK74_001602"
     CDS             complement(8185..8622)
                     /locus_tag="ACHK74_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFP0220444.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCQTLTRGSLAIPHSHLLS"
     regulatory      complement(8840..9015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(9104..10018)
                     /gene="pocR"
                     /locus_tag="ACHK74_001603"
     CDS             complement(9104..10018)
                     /gene="pocR"
                     /locus_tag="ACHK74_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HFP0220445.1"
                     /translation="MISAATLNSELINKIAQDFAQATSLAVVVVNIHGDEISELFNFT
                     PFCQMMRQHPQLSTRCRMSDRCGGLEASKSDQPCIYRCHAGLTDFSIPLVIAGHLVGF
                     VLCGQVRLRNDDVDLVDILNVDDCWQADPELLNEFRKVPEMDYSRVMASADLLKLIVE
                     NCLKKQLNFVVIKENKSRSDSVRQTRAPNPHDNKMKKALRYIDAHLSDDLRLEDVASH
                     VYLSPYYFSKLFKKYHGIGFNAWVNQQRMASARELLCHSDWSIASIARNLGFSQTSYF
                     CKVFRQTYQVTPQVYRQQVSENAAMETV"
     gene            complement(10221..11030)
                     /gene="pduF"
                     /locus_tag="ACHK74_001604"
     CDS             complement(10221..11030)
                     /gene="pduF"
                     /locus_tag="ACHK74_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFP0220446.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKLAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCVAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLVSIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGSLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            11542..11826
                     /gene="pduA"
                     /locus_tag="ACHK74_001605"
     CDS             11542..11826
                     /gene="pduA"
                     /locus_tag="ACHK74_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFP0220447.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAARNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            11823..12635
                     /gene="pduB"
                     /locus_tag="ACHK74_001606"
     CDS             11823..12635
                     /gene="pduB"
                     /locus_tag="ACHK74_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HFP0220448.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPETTHQTRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQGTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDRPSYI"
     gene            12654..14318
                     /gene="pduC"
                     /locus_tag="ACHK74_001607"
     CDS             12654..14318
                     /gene="pduC"
                     /locus_tag="ACHK74_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFP0220449.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            14328..14996
                     /locus_tag="ACHK74_001608"
     CDS             14328..14996
                     /locus_tag="ACHK74_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFP0220450.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVSSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            15011..15529
                     /gene="pduE"
                     /locus_tag="ACHK74_001609"
     CDS             15011..15529
                     /gene="pduE"
                     /locus_tag="ACHK74_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFP0220451.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            15539..17371
                     /locus_tag="ACHK74_001610"
     CDS             15539..17371
                     /locus_tag="ACHK74_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFP0220452.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALAETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGLTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPGGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLISWHRELAHGK"
     gene            17361..17711
                     /locus_tag="ACHK74_001611"
     CDS             17361..17711
                     /locus_tag="ACHK74_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFP0220453.1"
                     /translation="MESKEHTPAIVVTEIGDCISTWNEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWQAARKSPLLVGIACDREKLIVHYKNLPASAPLFTLMYQQDNHTRRSIGNNA
                     ARLVKGIPFRECHS"
     gene            17731..18006
                     /gene="pduJ"
                     /locus_tag="ACHK74_001612"
     CDS             17731..18006
                     /gene="pduJ"
                     /locus_tag="ACHK74_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFP0220454.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            18030..18452
                     /locus_tag="ACHK74_001613"
     CDS             18030..18452
                     /locus_tag="ACHK74_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFP0220455.1"
                     /translation="MKQSLGLLEVSGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            18455..19087
                     /locus_tag="ACHK74_001614"
     CDS             18455..19087
                     /locus_tag="ACHK74_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFP0220456.1"
                     /translation="MDKQTLQETVNQILDEMRERPIPLGVSSRHIHLSTADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            19084..19575
                     /gene="pduM"
                     /locus_tag="ACHK74_001615"
     CDS             19084..19575
                     /gene="pduM"
                     /locus_tag="ACHK74_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFP0220457.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRAAIQIHEALAWGLHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHRDRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            19579..19854
                     /locus_tag="ACHK74_001616"
     CDS             19579..19854
                     /locus_tag="ACHK74_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HFP0220458.1"
                     /translation="MHLARVTGAVVSTQKLPSLIGKKLLLVRRISADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            19866..20873
                     /gene="pduO"
                     /locus_tag="ACHK74_001617"
     CDS             19866..20873
                     /gene="pduO"
                     /locus_tag="ACHK74_001617"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFP0220459.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHIPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPGA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            20870..22255
                     /gene="pduP"
                     /locus_tag="ACHK74_001618"
     CDS             20870..22255
                     /gene="pduP"
                     /locus_tag="ACHK74_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
                     /protein_id="HFP0220460.1"
                     /translation="MNNSELETLIRNILREQLVPATPRSQRNAIFQTVDEAVCAAHQA
                     FLRFQQCPLKTRSAIISAIREELMPRLTELAEESAKETGMGNKEDKYLKNKAALDNTP
                     GIEDLTTTALTGDGGMVLFEYSPFGVIGSVTPSTNPTETIINNSISMLAAGNSVYFSP
                     HPGAKNVSLKLITTIEDIAFRCSGIHNLIVTVSEPTFAATEQMMAHPKISVLAITGGP
                     GIVAMGMKSGKKVIGAGAGNPPCIVDETADIVKAAEDIINGASFDYNLPCIAEKSLIV
                     VASVADYLIQQMQSFGALLLNYEQTEKLRAICLPDGSANKKLVGKSPSALLEAAGLPL
                     PAKAPRLLIAVVDANDSWVTCEQLMPMLPIVKVNDFDSALTLALKVEDGLHHTAIMHS
                     QNVSRLNLAARVMQTSIFVKNGPSWAGIGVGGEGFTTFTIATPTGEGTTSARTFARSR
                     RCVLTSGFSIR"
     gene            22266..23378
                     /locus_tag="ACHK74_001619"
     CDS             22266..23378
                     /locus_tag="ACHK74_001619"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFP0220461.1"
                     /translation="MNTFSLQTRLYSGPGSLKVISRFSHKHIWIICDVFLARSPLINT
                     LHQALPDSNRLSIFSEITPDPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGTGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     IVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRATKRYARLAKTCQLCPDNANDTASLNALIQHIEQLKTTCKLPTLANALKEKKAEWS
                     LCIPDMVQAALADATLRTNPRAADVSAIAELLEELL"
     gene            23375..24718
                     /locus_tag="ACHK74_001620"
     CDS             23375..24718
                     /locus_tag="ACHK74_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP0220462.1"
                     /translation="MNVGLISCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARVDTFL
                     VNAAECEPMLKVDQQLMAQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVSVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTVNGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGSLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRLQNDRSVLAVAKTVCEQCRLCTDLCPRHLIG
                     HELAPHLLVHAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLTPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            24721..25275
                     /gene="pduT"
                     /locus_tag="ACHK74_001621"
     CDS             24721..25275
                     /gene="pduT"
                     /locus_tag="ACHK74_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFP0220463.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSVASERAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            25275..25626
                     /gene="pduU"
                     /locus_tag="ACHK74_001622"
                     /pseudo
     CDS             25275..25626
                     /gene="pduU"
                     /locus_tag="ACHK74_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
     gene            25631..26074
                     /locus_tag="ACHK74_001623"
     CDS             25631..26074
                     /locus_tag="ACHK74_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFP0220464.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPIIGVVTKADLA
                     SMEQISLVKCWLREAGAHNVLVTNAVNNHGVTELFALLHTEDVCR"
     gene            complement(26175..26504)
                     /gene="tmaR"
                     /locus_tag="ACHK74_001624"
     CDS             complement(26175..26504)
                     /gene="tmaR"
                     /locus_tag="ACHK74_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFP0220465.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(26676..27734)
                     /gene="yeeA"
                     /locus_tag="ACHK74_001625"
     CDS             complement(26676..27734)
                     /gene="yeeA"
                     /locus_tag="ACHK74_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP0220466.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(27932..28405)
                     /gene="sbmC"
                     /locus_tag="ACHK74_001626"
     CDS             complement(27932..28405)
                     /gene="sbmC"
                     /locus_tag="ACHK74_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFP0220467.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEITGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQSKHH"
     gene            complement(28524..29690)
                     /gene="dacD"
                     /locus_tag="ACHK74_001627"
     CDS             complement(28524..29690)
                     /gene="dacD"
                     /locus_tag="ACHK74_001627"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFP0220468.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            29899..31326
                     /gene="sbcB"
                     /locus_tag="ACHK74_001628"
     CDS             29899..31326
                     /gene="sbcB"
                     /locus_tag="ACHK74_001628"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFP0220469.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(31369..31596)
                     /gene="tsuB"
                     /locus_tag="ACHK74_001629"
     CDS             complement(31369..31596)
                     /gene="tsuB"
                     /locus_tag="ACHK74_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFP0220470.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAGMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(31610..32668)
                     /gene="tsuA"
                     /locus_tag="ACHK74_001630"
     CDS             complement(31610..32668)
                     /gene="tsuA"
                     /locus_tag="ACHK74_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFP0220471.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYLAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(32847..34205)
                     /gene="plaP"
                     /locus_tag="ACHK74_001631"
     CDS             complement(32847..34205)
                     /gene="plaP"
                     /locus_tag="ACHK74_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFP0220472.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(34195..34257)
                     /gene="yoeI"
                     /locus_tag="ACHK74_001632"
     CDS             complement(34195..34257)
                     /gene="yoeI"
                     /locus_tag="ACHK74_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFP0220473.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(34472..35401)
                     /gene="yeeY"
                     /locus_tag="ACHK74_001633"
     CDS             complement(34472..35401)
                     /gene="yeeY"
                     /locus_tag="ACHK74_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0220474.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(35447..36271)
                     /gene="yeeZ"
                     /locus_tag="ACHK74_001634"
     CDS             complement(35447..36271)
                     /gene="yeeZ"
                     /locus_tag="ACHK74_001634"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0220475.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGSGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(36354..36608)
                     /gene="yoeB"
                     /locus_tag="ACHK74_001635"
     CDS             complement(36354..36608)
                     /gene="yoeB"
                     /locus_tag="ACHK74_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFP0220476.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(36605..36856)
                     /gene="yefM"
                     /locus_tag="ACHK74_001636"
     CDS             complement(36605..36856)
                     /gene="yefM"
                     /locus_tag="ACHK74_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFP0220477.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            37140..37190
                     /gene="hisL"
                     /locus_tag="ACHK74_001637"
     CDS             37140..37190
                     /gene="hisL"
                     /locus_tag="ACHK74_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFP0220478.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    37166..37289
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            37336..38235
                     /gene="hisG"
                     /locus_tag="ACHK74_001638"
     CDS             37336..38235
                     /gene="hisG"
                     /locus_tag="ACHK74_001638"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFP0220479.1"
                     /translation="MIDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            38241..39545
                     /gene="hisD"
                     /locus_tag="ACHK74_001639"
     CDS             38241..39545
                     /gene="hisD"
                     /locus_tag="ACHK74_001639"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFP0220480.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAVLPRAETARQALSASRLIVTNDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            39542..40612
                     /gene="hisC"
                     /locus_tag="ACHK74_001640"
     CDS             39542..40612
                     /gene="hisC"
                     /locus_tag="ACHK74_001640"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFP0220481.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKVVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFNSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            40612..41679
                     /gene="hisB"
                     /locus_tag="ACHK74_001641"
     CDS             40612..41679
                     /gene="hisB"
                     /locus_tag="ACHK74_001641"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFP0220482.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            41679..42269
                     /gene="hisH"
                     /locus_tag="ACHK74_001642"
     CDS             41679..42269
                     /gene="hisH"
                     /locus_tag="ACHK74_001642"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFP0220483.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            42269..43006
                     /gene="hisA"
                     /locus_tag="ACHK74_001643"
     CDS             42269..43006
                     /gene="hisA"
                     /locus_tag="ACHK74_001643"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFP0220484.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEDDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            42988..43764
                     /gene="hisF"
                     /locus_tag="ACHK74_001644"
     CDS             42988..43764
                     /gene="hisF"
                     /locus_tag="ACHK74_001644"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFP0220485.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLDDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETAKYHVNQYTGDETRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            43758..44369
                     /gene="hisIE"
                     /locus_tag="ACHK74_001645"
     CDS             43758..44369
                     /gene="hisIE"
                     /locus_tag="ACHK74_001645"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFP0220486.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(44466..45443)
                     /gene="wzzB"
                     /locus_tag="ACHK74_001646"
     CDS             complement(44466..45443)
                     /gene="wzzB"
                     /locus_tag="ACHK74_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFP0220487.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDEKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(45589..46755)
                     /gene="ugd"
                     /locus_tag="ACHK74_001647"
     CDS             complement(45589..46755)
                     /gene="ugd"
                     /locus_tag="ACHK74_001647"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFP0220488.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPNTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPHVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELRDVADKVYTRDLFGSD"
     gene            complement(47004..48410)
                     /gene="gndA"
                     /locus_tag="ACHK74_001648"
     CDS             complement(47004..48410)
                     /gene="gndA"
                     /locus_tag="ACHK74_001648"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFP0220489.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSVFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT        12733 a        12559 c        11639 g        11485 t
ORIGIN      
        1 gttcttcttt ttctgattac agaaggcaaa gtggcaataa cgcatagttt cccgataaag
       61 acgcgatagc gacatcccgc ataaggcatt tttctcttta tctttgtacg gtacttcatg
      121 gaacagagtt tttgaccttg cgaatcgtga tgtctgttgg gggcaacttg ctcactgaag
      181 cgtgagactc gattaagcgc acgaaacaca gaaatcaaaa aacccggtca cttttttaca
      241 aggtaaccgg gtaaaaataa tttttatttt ttaactgttt tgagactcat agagatgtct
      301 caaaactaaa atttggctcc tctgactgga ctcgaaccag tgacatacgg attaacagtc
      361 cgccgttcta ccgactgaac tacagaggaa tcgtgagaac gaggcgaata ttagcgatgc
      421 ccacccataa tgtcaaagcc tgttttttaa atttgaaatc atttgctgaa ataatctgca
      481 ttttgtcgtt tattccgaca cagctggctt tttttcacac ttttgcggtt cggatcgagg
      541 gtatttccac agccaacgtc cagtaaccat tcgccagtaa aacagcacgg cccgcacagc
      601 ccagtcggca aacattccca tccagacacc aaccacaccc cagccaagca tgatgcccag
      661 cacataaccg acgacaaccc ggcaacccca catgctcaac atagaaaccc acatggcgta
      721 acgggcatcg cgagcacctt taaatccagc gggtagcacc catgaggcgg accaaatagg
      781 cataaataaa gcatttagcc aaatcagaat cacaacgaca tgtttaacct gtggatcctg
      841 ggtgtaaaac gatgccataa ccccggcaaa gggagccgtt agccaggcga tggccgttaa
      901 tccaagagtg gaaagccaga acacatgccg caactgaatc tctgcttgcg ctatctgccc
      961 tacccccaac cttcggcctg taatgatcgt agaagcagag ccgagcgcac ttccgggtaa
     1021 gttgataaga gccgcaattg aaaacgcgat aaaatttccg gcaataacac tggtccccat
     1081 cccggcaacg aacatttggg ttaataaccg accactggta aataacactg attcgacact
     1141 cgctggaata ccaatcccca tgacttccca gataatgcta aaattcagcg gtttaaaata
     1201 gctctttaac gaaatcctta gcgcaggatt aaaaccaatc gccagcaccc acaaaattgc
     1261 aactgcgcca atataacgag aaatggttaa acccaaccca gccccgacaa atcccagtcc
     1321 cggccaggag aaaaggccgt aaatcaatat gccgctaata ataatattaa gaatattcag
     1381 gctaccgtta atcagtagcg gtattttcgt attccctgca ccacgaagtg ccccgctacc
     1441 aatcaaagtg atggcagctg ctggataact gagtaccgtc agctccagat aagtcaacgc
     1501 cagtgcttta acttctgtcg tggcatcacc cgcgacgaaa tcaataattt gttcgccaaa
     1561 atgatgaata agcgttgcca acagtacggc aaacaacgtc atgatcacca atgactgccg
     1621 cgtcgccacc ctcgctcgtc gtcgatcccg cttaccgaga ctaaatgcca caacgacagt
     1681 agtaccaaga tcgatagctg caaaaaaagc cataatgacc atattgaagc tgtccgccaa
     1741 tcccacgccg gccatcgcat cttttcccag ccagctgacc agaaaagtgc tcaggacacc
     1801 catcaacagg acacaggcat tctccatgaa gataggaaca gcaagcgggg ttatctcgcg
     1861 ccagaacaac actttgtagc tcttgcgttt agcgtgccag cgagtgccgt gaacaacctg
     1921 gcgtaaagca gaggagatat tcaaagccga ccttaattgc agaaagtgaa accacatttc
     1981 aaataatgag ggagaatcag caaagctgca aagattttcg ccaacaaatt gtctgcaaat
     2041 gcaacaaact gttgatagaa acggcaaaca gttggggaat ttaaaaatcg ggtttgacaa
     2101 aagatttttc gccgttaaga tgtgcctcaa cagcgattcc tctgtagttc agtcggtaga
     2161 acggcggact gttaatccgt atgtcactgg ttcgagtcca gtcagaggag ccaaatttct
     2221 gaaaagcccg cttttatagc gggctttttg ctatatctga taatcaattt cctcttcact
     2281 gttttccatc acctgccgct tgatatcctc aactgacagt cctgcattac aaagttccag
     2341 aaagcgccag acatagttac gctgaagttg tcctcgcttc agtcccaacc agacagtatt
     2401 agcatcgaaa agatgccgcg tatccagacg gattaaattc tcttcctctt gttcgccgct
     2461 ggattgttcg gcaactaatc cgatcccaag cccaagagca acataggttt taatgacatc
     2521 agaatcctgc gcacttaata caatatctgc cagcaaacct ttgcgggcaa atgcgtcatc
     2581 aatacgtgag cgccccgtaa tcccccgtcg gtaagtgatt aacggccact tcgctattga
     2641 ttccagcgtc aatggtgtaa tttgcgttaa gggatgatcg agtggaacaa gcaaactatg
     2701 gtgccaacga aaccacggga aggcgacgag ctgcgggtca ttactcaaac gctcgctggc
     2761 gataccaata tcagcttcgc cattttgcaa caacgtcgca atttcctgtg gcgtcccctg
     2821 gattagctcg agccgaacct ccgggaaaag ttcgcgaaaa gctttaatga cctctggcaa
     2881 gctataacgt gcctgagtat gcgtcgttgc aatagtgaga acgccagacg tatcgttggt
     2941 aaacaggtct gcaagccgac gaacattact ggcttcattc agaatacgtt ctgcaatgac
     3001 cagtaatgct ttgcccggtt cagtcatgcc cagcagtcgc ttaccccgtc gaacaaatat
     3061 ttcgatgcca agttcatcct ccagttcccg aatatgacgg ctgacgcctg actgtgaggt
     3121 aaaaagcata ttcgcaacct ctgtcaggtt gtaatcctga cgtgcagcct cgcggattat
     3181 ctttagttgt tggaaattca cggtaaactc cgggcagttc agatttcccg ttattgttaa
     3241 agtctaatgc ccggcataac aaataataaa aacccgcatc ttattccatc ccgatataac
     3301 acttagctca ccaattgcca ctgccttttt tccatcactg gagaactaat cactgacatt
     3361 aacaactctt tcactgcctg tgcctgtggc gataagttcg ctctggcggg taaatttaat
     3421 gacaaagaga gactcatgga aggagtggta atgcgtgaca tccacccatt tactgcgcca
     3481 cataacgaac gcgcggccga ttcgggtaat actgcaacgc ccatgccgct ggcaatcgct
     3541 gcggtaagcg tggcaataga ctcaatttca ccaataactt ttgccgtgag tcgccgtagg
     3601 gaaaaagcct catcaacacg aagtctaata gcactgtaat cactggggag aaagaggttc
     3661 attttcgcaa tagcattcac atcaacgctt tgtcccaggc aatcttgagt tcctaccaga
     3721 aaaagatctt ctttcagcaa agcctgactg gatacaccag ccacagggga atgctcataa
     3781 atcaccgcca tatcgagttg gtgatttatc aatttttcgt taagcactgc accactattt
     3841 tcatgaagat agataacgat ctccggaaat tcagcgcgaa ccgcctgtaa taagggcatg
     3901 gtgatggatg acgcagcggt tcctggtgca aagccaatcg agacttgccc cgataatgcc
     3961 tgaccaacgt tatgcaccgc cagttgggcc tgttcacact gacgtaaaat ggcccgcgca
     4021 tgggtataga gaatttttcc ggcgtctgtt ggcgtaacgc cccgctttgt acggatcaaa
     4081 agttgttgat ttaactcacc ttccagtgtg gcaacctgct ggctgagcgc tggttgtgcg
     4141 atatgcaata cttcagcagc ctgggtcagg ctaccaatat ctacaatttt tacgaagtat
     4201 ttcaggcgtc tgaagttcat gttgcctccg gtttttaaga atcggcccaa gtgccgccat
     4261 tacttacaac cagattgcaa gatgcttgcc agttttattt tggcgttgat gtacaagcta
     4321 accaactgtc aaataagaga ttatgataga ttcgtcattt gctcctttaa tcagctgtcg
     4381 cgttcccctg ccctataaaa ggagggtatg caccacgatg gttcattacc caataagatt
     4441 gaaagctcac cactttgttg aaattgacag caaacaaaca aaaaaatgca tttcaccctt
     4501 tgacatcacc atgcactgcc attaatatgc gccccgttca cacgattcct ctgtagttca
     4561 gtcggtagaa cggcggactg ttaatccgta tgtcactggt tcgagtccag tcagaggagc
     4621 caaattcaaa aaagcctgct ttctagcagg ctttttgctt tctaattacc aacgctctta
     4681 aaacatctgt cttgaaccag aactaatttg cacaggcatt cccgatcgac gttgcaacgc
     4741 agcatttgcg cgatttacat caacttcttg cccgctgata aacgcccgca aagatggggt
     4801 taccggcaat ggcacttttc ggtcagactc atattctgca cgattgcgcg acaacggctc
     4861 atgaacttcc agccagttcg agccatctgg ttcagtggtg tattttactg gctggtcgat
     4921 aatttgcaca cgcatcccaa caggaacatt atcaaacaga tatttgatat cgtcattgcg
     4981 cagacgaata cagccctgac ttacccggag cccaatacca aaattggcat tggtaccatg
     5041 gatggcatac aacctgccaa tataaatcgc gtacagcccc atgggattat cggggcccgc
     5101 aggaacaaat gcgggcaaac tctcccctcg tttcgcatat tcgcgccgag tgttcggcgt
     5161 tggcgtccag gttggagctt cttgtttacg ttcaacggta gtcacccagt tacgcggggt
     5221 ttctcgccca gcctggccga taccaatagg aaagacttcc acagtattac tgtctggtgg
     5281 gtagtaataa agacgcatct cagcgacgtt aaccacaatc cctttacgaa cagtggcggg
     5341 caaaatcagt tgctgcggaa tggtgagttg cgagccagac ttcggcaaaa aaacatcagc
     5401 gcccgggttc gcttccagca tgttacttaa cccttgcccg tattgtgcgg caaaagtctc
     5461 cagcggctgg gtattgtgat caggaacagt tacagtaaac gactgcccca ctaaacggct
     5521 accctctgga ggtaatggat aagttacagc caggctagta tggctggcaa aaagcagagc
     5581 aaatgagcaa aaaatattta cacgacgcat catgtccctt tcctaagtcg cgaaagctat
     5641 ccgttaagta tagcttttat cagacttttc gtttttaact gttcaaatca gaagtcgtat
     5701 tccccggtag aacaatatta ctggcagcaa gttcgcccat gttgttatat atcgcacagg
     5761 cagcttcgat gatgggcatc gccagagctg cgccactccc ctcacctaaa cgcatctcca
     5821 tattgagata aggctccagc cccaaatgcg acagcgcaat acgcgcccct ttttctgccg
     5881 acaggtgaga aggaatgaga tacggtttga ttgcaggaga catctggcag gctgcgagcg
     5941 cggcagcata agaaagaaat ccatccagca acacgggtaa accgcaggaa gcagcgccta
     6001 acatcacgcc agccattccg accaaatcaa atccacccac ttttgccagg acatcaacgc
     6061 catcctgcgg atttggttga ttcaacgtaa tcgcccggcg cacaacatca attttattag
     6121 ccagtttatc tgtcggcagg tttgcgccaa tcccaaccac ttcttcagga gcccggccag
     6181 tgattgtgct gactattgcc gctgccggtg tcgtgtttgc catccccagt tcacctacac
     6241 caaacagcgt aacaccgttt tttgccagct cccgcgtata acatatgacg tccaaaagca
     6301 acttttctgc ctgatggcga ctcattgccg gagctgaagc aatattgcca ctacctcgag
     6361 caacacgcat gttgataagc ccggggatag gctcagcagt atcaatacca acatcaatta
     6421 cgtagacgtt agcgcccgct tgtgctgcca gcacacacac gccggttgtt ccgcgggtca
     6481 tattttcagc ctgaatggct gtcacttctt ttggggaaat agcgacccct tcctcccaga
     6541 cgccgtgatc ggcacacata accagtaccg cttttttgcc cacatgcggt atgccattca
     6601 accccggcat tcctgccagt tgtatggcaa gcgcctccag ccttcccagg ctaccaacag
     6661 gtttgagtaa cccgtcaata tgccgttgtg cacgcgacat cgcggcagga tcgatggcag
     6721 ggatcgtatt cagtaaatcg gcaagtgttt gcatgtcacg tctctttata gcagtgccag
     6781 caggaagacc agttcaccaa gctctatagc agctcccagc gtatcgccgg tttgcccgcc
     6841 cagcgtacgt ttgagaagct gaccgaggat gaaaatcgcc accatcgtca ccaccatagc
     6901 agccacacca tgcatacccg gcaataatac ggcagcaaaa atagcggcta agccgagtgt
     6961 gacgcaggtt tgtcgcccat caattttgcc aatgaacaca ttgccaagcc cttcttcccg
     7021 ggcataacga tgacgataca tcaataatgc ggcagtcccg cggctgaccg cacatgctgc
     7081 cgccagcgat gcaaggatcg gttcgccacg cagagccaac tcgcttaaca ccagaatctt
     7141 tgccagtacc acaaaaatca atgccagacc gccgtgggtg cctaaacggc tatcacgcat
     7201 gatttccaac atgcgatcgc ggctacgtgc agaaaatacg ccatcacagg tatcggcgag
     7261 gccgtccagg tggaatcccc cggtcatcag cgccagcaca agcacgctaa acagtgccgc
     7321 cagtggtacg ccacaccatg cctgcagcac catgaagacc agcccgctaa tcgcgccaag
     7381 caataatcca attaaaggaa aagtaataat accgcgagaa taatgctcga aatccagtcc
     7441 ctgggaccag cgacgcggta caggcagacg cgtaataaaa gagagcatcg cccaaaataa
     7501 tttactcatt tgatttttac tccaataccc gaaaccacca accatacttc atttgccgca
     7561 gcggccagct gctgatttac ccgcccggca atatcacgaa aatgtcgtgc cagacgactc
     7621 tccggcacaa tccccattcc cacttcgtta gtcactaata caacctttgc gggacaacgt
     7681 tggcaggcag caatcaacga ctgaatctca gcattaatcg cctgttccat cgcctgataa
     7741 tcccattcat cagggtcttt atcgccgcca taatcaaaca acagattagt caccatggtg
     7801 gtaacgcatt ccagcaatac agcctcatgc gggttaatgt ctgcatgaat taattcatca
     7861 agatgttgcc aacgctccac cgtgcgccag tgcgccgggc ggccttgccg atgatgttct
     7921 atccgtgcag ccatctcatc atcaaggatt tgtgaggtgg cgatatacag aacctgtgaa
     7981 gagtccccaa taagcgcctc tgcatggcga ctcttcccgc tccgtgcgcc ccccgtcacc
     8041 aaaatcatca taccgactcc tgattcaatt gtatgatggt attcattgaa cagaacaatg
     8101 gatcccccac cgttgctgac cgcaaaacat ttacgcgatt aatactgcgc gtaatataat
     8161 taaaaatcca gcattctcaa tccattacga aagaagatgg ctatgtggaa tagccagact
     8221 tcctcgtgtt aacgtctgac aaaatgcgtc attgacagcg gtttctctgg aatgacagat
     8281 ttcattcaga taatggatta atgctacagc cccaccagcc ggaccagtca gcaaacgggc
     8341 attgtgttct gcaatcaaaa ttaccgtcgt tccctcagaa tgagcgagcc attctggtac
     8401 ggatatctcg ctttcagctg ctatcaatag cagcactgaa taatcgctat acgcatctga
     8461 taacgattgc acaggcccac catcaagagg ctggaaatct tgctgccagt cctcaccaac
     8521 aaaacaggtt attggcaata tttcagccag atgtttcgcc agatccacat ctccagataa
     8581 gaggcaaaaa agagctatcg attcgggttt tatcgttttc atgcctctcg ccctccactt
     8641 aatttcctgt aatggcggtc tggttgataa gccatgaaac aatattgcca caccttgaat
     8701 gcagccctgg tgcctgtcgg cactgcgatg aaagaactaa atgatgccgc cataccctga
     8761 tcctttcagg gtggtagggg tataaagacg cgccagtact ctaccggacg cttccgggca
     8821 acccacttcc caccgaagtt gttggtattg actgacagac tggtcttctg gcttagcgtc
     8881 atcctcaccc gaccttccca atctcgcgat cagtggcatt ttcgggtttg tcagcattac
     8941 agcagcgggg gctgcgaggg attttcaccc tcttcccagg cacaaatgtg ccacacctgt
     9001 cagcgtatgt tatgagctga tttcactttt cccataacac gtaaataacg ttaattattc
     9061 attccagcgt ttatttaaac cttccctttt tttagcattt attctatacc gtttccatcg
     9121 ccgcattttc actcacttgc tgtcgataca cctgcggcgt aacctgataa gtctgacgga
     9181 aaactttgca aaaataactg gtttgcgaaa aacctaaatt acgcgcaatg ctggcgatgc
     9241 tccagtcact atggcacagt aactcacgag cgctggccat ccgttgttgg tttacccacg
     9301 cattaaaacc gattccatgg tatttcttga ataacttact gaaataataa ggacttagat
     9361 aaacgtgcga ggcgacatct tccagacgca aatcgtctga taaatgcgca tcaatatagc
     9421 gtaaagcttt tttcatttta ttgtcgtggg gattaggcgc acgagtctgg cgcaccgaat
     9481 ctgaccgaga tttgttctct ttaataacca caaaattgag ttgttttttg aggcaatttt
     9541 ccacaatcaa cttcaacaaa tcagcagagg ccataactct tgagtaatcc atctcaggaa
     9601 ctttgcggaa ttcattaagt aattcgggat ctgcctgcca gcaatcatcg acattcagaa
     9661 tatcgaccaa atcgacatca tcattacgca gacgaacttg cccgcacaat acaaaaccga
     9721 ctaaatgtcc ggcgattacc agtggaatag agaaatcggt taatccggca tgacagcgat
     9781 aaatacaagg ctgatcggat ttagaggctt ccaggccccc acaacgatcg ctcatgcgac
     9841 atcgagtgct tagctgaggg tgttgccgca tcatctgaca aaatggagtg aagttaaaca
     9901 gctcggaaat ttcatcgccg tgaatattga ccacgacaac cgcaaggctg gtcgcctgcg
     9961 caaaatcctg cgcgatttta ttaatgagtt ctgaattaag tgtcgccgca gaaatcatga
    10021 taaaacctcc cagttaattt attgttatag caaaacaatt gttttacatt taatcttcac
    10081 atcatccctg atgatttact gacgagaagc acaaaggaca ataacaaaag cctccgtgaa
    10141 gcgggagact ttttaataag ttcggaaagc agatcacaaa cagtaaaaaa ataaatgttt
    10201 ataactgcca gagtatttcg ttattcgcta attcctttag gtgtaacact attgacaggt
    10261 aaattctttg caatgagata gcgataaatc gccgcaccaa cacatgcccc aatgatcggt
    10321 gcaataatgg gtacaatgaa ataaggaata ttgcgtccac cagtcatcgc aatttctccc
    10381 caccccgcaa gccaggtgaa aatcttaggt ccaaaatcac gcgctggatt catggcaaaa
    10441 ccggttaatg gtccggtaga agcaccaata acagcaacca gaataccaat aagtaaaggc
    10501 gcaagcgatc ctttgggtac gccattaccg tcatcggtca acgccataat caaccccatt
    10561 aacatggacg ttatgaccac ttcgaccaac gcagcttgcc atacattaat cgccgctgcc
    10621 ggataagtac tgaagatact gacgagttgt aaactttctg cactaccacg caccatattg
    10681 tgagcagatt caaaatcggt aaacaaactg ctgtatagag aatatgccag aaccgcacca
    10741 ccaaaggcac ccgctaattg cgccacacaa aatggcagca ctttacgggc tgggaaagag
    10801 gcaaacagcc acagagcaat ggtaacagcc gggttgagat gagcgccgga aatacctgcc
    10861 gtaaggtaaa cagcaagcga aatccctaac ccccaaatga tacaaatttc ccacagtccc
    10921 agacttgccc cggcaagttt cagggcgcac aaacaaccga tgccaaaaaa gaggaacagt
    10981 ccggtgccta aaaactcggc aacgcattgc gccttgagtg aatcattcat agtgacacct
    11041 tctttacgga gtttttcttt atcagccaac tatgcgctga ttgttgtgta taccgagcgc
    11101 aactataaaa aattgctctg cagaaaatat tgccaacatg catgacagac tcgtgtaaaa
    11161 atcgtgctaa cataacaaat tattgtcatt gccgaatcat gcaacacagc gttaatcagt
    11221 gttgaaagta ataattactg tcaaactttc acaataaaaa attgccatca tgatataata
    11281 aaattgaaac aaatatcaca gttccttcct cgaactaaac tcaacgaaat attactcgcg
    11341 attaaactca ctagccaacg caattaattc acattgaaat atccacaaaa caactaataa
    11401 attaacaaca tatagttcat aaaagcaatt ttataatatt tgttctctat taatttttct
    11461 cattagccga ttaagtcagc cattcttttt cctcgctttt ttttatagtc atatcgtcaa
    11521 aaaaatccag gcgaggtctt tatgcaacaa gaagcgttag gaatggtaga aaccaaaggc
    11581 ttaactgccg ccatagaggc cgcagacgcc atggtgaagt cggcaaatgt tttgctggtc
    11641 ggctacgaaa aaataggttc cgggctggtg accgtcatcg ttcgtggtga tgtcggggcg
    11701 gtaaaagcgg caacagacgc aggtgccgca gctgcgcgta acgtcggtga agtaaaagcc
    11761 gtacacgtta ttccccgccc acataccgat gtcgaaaaaa tcttaccaaa gggaattaac
    11821 ccatgagcag caatgaactg gtcgaacaga tcatggcgca ggtcatcgct cgcgtagcga
    11881 cgccagagca tgaggttctc ccggaaacca ctcatcaaac acgagagacg gctatggcag
    11941 aaaaaagctg cacgttaacg gaatttgttg gcaccgctat tggcgacact gtaggtctgg
    12001 tcattgcgaa cgttgatagc gccctgttgg acgcgatgaa acttgaaaaa cgctatcgtt
    12061 ccattggcat tctgggtgcg cgtattggcg cagggccaca cattatggcc gctgatgaag
    12121 cggtaaaagc caccaacact gaagttatta gcattgaact tcctcgggat actaaaggcg
    12181 gtgccggaca tggctcattg atcctcttag gtggcaacga tgtttctgac gtgaaacgcg
    12241 gtattgaagt cgccctgaaa gaactcgatc ggacctttgg cgatgtctat gccaacgaag
    12301 ccggacatct tgagctgcaa tataccgccc gcgccagcta tgcactggaa aaagcgtttg
    12361 gtgcaccatt gggacgggct tgcggcgtta ttgtcggcgc tccagccgct gtgggtgttc
    12421 tgatggccga caccgcgctg aagtccgcca atgtggatgt tgtggcatac agctcccccg
    12481 ctcaaggcac cagctacagc aatgaagtca ttctgattat ctcgggtgac tccggcgcag
    12541 tccgccaggc ggtcatctcc gcacgcgaaa tcggtaaaac cgtgctttcg actctcggcg
    12601 atacaccgaa aaacgatcgc ccttcctaca tctgaattct ctgaggcaga tttatgagat
    12661 cgaaaagatt tgaagcactg gcaaaacgcc cggtcaacca ggacggtttc gttaaagagt
    12721 ggattgaaga aggctttatt gcgatggaaa gcccgaacga tcctaaaccg tcaatcaaaa
    12781 ttgttaacgg cgtcgttacc gaactggatg gaaaacccgc cagccagttt gacctgatcg
    12841 accactttat cgcccgctac ggcatcaatc tcgcgcgcgc cgaagaagtc atcgcgatgg
    12901 attcggtaaa acttgccaat atgttgtgcg atccaaatgt caaacgtagc gatatcgttc
    12961 cccttaccac agcaatgacg cctgcaaaaa tcgtcgaagt ggtttcgcag atgaacgtgg
    13021 tagagatgat gatggcgatg cagaaaatgc gcgcccgtcg aacaccatca caacaggctc
    13081 atgtcaccaa cgtcaaagat aacccagtac aaattgccgc cgacgcagct gaaggcgcat
    13141 ggcgcgggtt tgatgaacag gaaaccaccg ttgccgttgc tcgctatgca ccgttcaacg
    13201 ccatcgccct gctggtcggc tcacaggtgg gacgtccggg cgtcttgacc cagtgttcac
    13261 tggaagaagc caccgaactg aaactcggta tgttaggcca cacctgttac gccgaaacaa
    13321 tttctgtcta tggtacagaa cctgttttca ccgatggtga tgataccccg tggtcaaaag
    13381 ggtttctcgc gtcatcctat gcttctcgtg gcttaaaaat gcgctttact tccggctccg
    13441 gctctgaagt acagatgggt tacgcggaag gcaaatcaat gctttacctc gaggctcgtt
    13501 gcatttacat caccaaagcc gcaggcgttc agggtctgca gaatggttcg gtaagctgca
    13561 tcggtgttcc atcagctgtg ccatcaggca tccgcgcagt attagcggaa aacttaattt
    13621 gctcatcgct ggatctggaa tgcgcctcca gtaatgacca gacctttacc cactcggata
    13681 tgcgccgtac cgcacgtttc ctgatgcaat ttctgccagg caccgacttt atctcttccg
    13741 gtttttccgc tgtgccgaac tacgacaaca tgtttgctgg ttcaaacgaa gatgctgaag
    13801 acttcgatga ttacaacgtc attcagcgtg acctgaaggt tgatggtggg ctgcgaccag
    13861 tacgcgaaga agatgttatc gctatccgca ataaagccgc tcgcgcttta caggctgtct
    13921 ttgccggtat gggactaccg cctattaccg atgaagaagt cgaagcggca acctatgccc
    13981 acggttcaaa agatatgcct gagcgcaata ttgtggaaga catcaagttc gcccaggaga
    14041 ttatcaataa aaaccggaac agcctggaag tggtgaaagc cctggcgcaa ggcggtttta
    14101 ccgatgtcgc ccaggacatg ctcaacatgc aaaaagcaaa actgaccggc gattatctcc
    14161 atacctcagc catcatcgtc gatgacggac aagtgctctc tgcggtcaat gacgtcaatg
    14221 attacgccgg accggctaca ggttaccgcc tgcaagggga acgctgggaa gagatcaaaa
    14281 atattcccgg tgcacttgat cccaacgaaa ttgactaagg gtgagaaatg gaaattaacg
    14341 aaaaattact gcgtcagatt atcgaagacg tgctggcaga aatgcaaccc agcgacaagt
    14401 ccgtttcatt tcgcgcacct gtctctgcaa ctgtctcttc cgcgccagat accggaaatt
    14461 ttctgaccga aattggcgaa gcccaacaag gcactcagca agatgaagtc attattgctg
    14521 taggcccggc gttcggcctg gcgcaaaccg tcaatatcat cggtattccg cataaaaaca
    14581 tcttgcgcga agtgattgcc ggtatcgaag aagaaggcat taaagcccgt gtgattcgct
    14641 gctttaaatc gtccgatgtc gcattcgtag cagtcgaagg cgatcgtctt agtggttcag
    14701 gtattgctat cggtattcag tcgaaaggca caacggttat ccaccagcag ggacttcctc
    14761 ccttgtctaa cctggaactt ttcccacaag cgcctttgtt gacgctggat acttatcgtc
    14821 agattggcaa aaacgccgcg cgttatgcga agcgcgagtc tccacaaccg gtgccgaccc
    14881 tcaacgatca aatggcgcgg ccgaaatacc aggccaaatc tgccattttg catattaaag
    14941 agacgaagta cgtggtcact ggcaaaaaac cgcaagaact gcgcgtgacg ttttaacaaa
    15001 ggaaaccttc atgaataccg atgcaattga gtcgatggtc agggacgtgt tgaaccgcat
    15061 gaacagttta caggatgcag ctcctgtttc tgcggtcccc aacgcatcaa tactgagcgc
    15121 caaagtgacg gattacccgc tggcaaataa acatccggaa tgggttaaaa cagcgaccaa
    15181 taaaacgctc gacgatttca ctctggaaaa tgtgctaagc gataacgtca ccgccctgga
    15241 tatgcgcatt acccctgaaa cattgcgtat tcaggctgcg attgccagag atgctggtcg
    15301 cgatcgtctg gcgatgaatt ttgaacgcgc cgctgagctt acctcagtac cagatgatcg
    15361 catcctcgaa atctacaatg cgttacgccc ttatcgttca actaagcaag aacttattgc
    15421 cattgctgac gatcttgaac agcgttatca ggccaaaatt tgtgctgcat ttgtccgtga
    15481 agctgcggaa ctttatgtcg agcgcaaaaa actgaaaggc gacgattaag gacgaactat
    15541 gcgatatata gcaggcattg atatcggcaa ctcatcaaca gaggttgcgc tggcaacgct
    15601 cagtgcaact ggagagttgt cgttcgtgag tagcgccctg gcggaaacca ccggaattaa
    15661 aggtacgttg cgtaacgtcc atggtattca ggaggcgctc gcacaggcaa cgaaaaaagt
    15721 cggcatcaat gttagcgata tttcgcttat tcgtatcaac gaagcaactc cggtcattgg
    15781 tgatgtggcg atggaaacca tcactgaaac tatcatcacc gaatcaacca tgatcggtca
    15841 caacccgaaa actccgggcg gagtggggct gggcgtaggg ctcaccatca ctccgcaaga
    15901 gttattaaca cgcccggcgg acacacctta catcctggtg gtgtcatcgg catttgattt
    15961 tgccgatatc gcgacgatga taaacgcctc cgtacgcgcc ggatatcagc tgactggcgt
    16021 tattctgcaa cgcgacgatg gcgtactggt caataaccgc ctggaaattc ctttacctat
    16081 tgtggatgaa gtcctctaca tcgatcgtat tccgctcggc atgctggcag caattgaagt
    16141 tgccgttccc gggaaagtta tcgaaaccct gtctaacccg tatgggattg ccaccgtatt
    16201 cgccctcaat gctgaagaga ctaaaaatat tgttcctgtc gcgcgggcgc taatcggcaa
    16261 ccgttcagca gtggtagtga aaacgccctc tggcgacgtt aaagcccgtt cgatacccgc
    16321 cggaaatatc gaactgcttt ctgccgggcg cacaacgcgc gttgatgtag ctgctggtgc
    16381 cgatgccatt atgaaagcgg tcggtgagtg cccaaaactg gaaaacgtga cgggagaacc
    16441 aggcaccaac atcggcggaa tgctggaaca tgttcgccag accatggcgg aactgaccaa
    16501 caaacccagc aacgaaattt ttattcagga tttgctggcg atagatacct ctgtccccgt
    16561 cagcgtaacc ggtggtctgg caggtgaatt ttcactggaa caggctgtgg gtattgcctc
    16621 tatggtgaaa tccgaccgtc tgcaaatggc gatgatcgcc agtgaaatta agcaaaaact
    16681 gcatgttgat gttcaggtcg gaggcgcaga agcagaagcc gccattcagg gggccttgac
    16741 tacccctgga accacacgtc cactggcaat tctggactta ggtgcaggct ctactgatgc
    16801 ctccatcatc aatcagagtg gggaaatcgt tgccactcac cttgcgggag cgggcgacat
    16861 ggtaacgatg attatcgctc gtgagctggg cttaaatgat cgttatctgg cagaagaaat
    16921 caaaaaatac ccgttggcaa aagtagaaag cctgttccat ttacgccatg aagacggaag
    16981 cgttcagttc ttccctactc cgctgtcccc tcatgtattt gcccgcgttt gtgtggtgaa
    17041 acctgacgaa ctggtgccta ttcccggcga tttaacactg gaaaaagtac gtgctgttcg
    17101 ccgcagtgcg aaagagcgcg tcttcgtaac caatgccctg cgcgcgctac gtcaggtaag
    17161 tccaggcgga aatattcgcg atattccgtt cgtcgttctg gtgggcggtt cttccctgga
    17221 tttcgaagtc ccgcagttag tcaccgatgc attggcgcat tatcgattgg tcgccggacg
    17281 ggggaatatc cgtggtagtg aaggcccgcg caatgcggta gctaccgggc tactgatttc
    17341 ctggcacagg gagttggctc atggaaagta aagaacatac gcccgctatt gtcgttacgg
    17401 aaataggtga ctgtatcagt acatggaatg aggtgcttct tggcattgaa gaggaaggca
    17461 tcccctttcg cattcaacat atcccgtcag gtgaagtcat cgatagcgcc tggcaggctg
    17521 ctcgtaagtc ccccctgttg gttggcatag cctgcgaccg ggaaaagctg attgtgcatt
    17581 acaaaaactt accggcatca gcgccactgt ttacgctgat gtatcaacaa gataaccata
    17641 cccggcgcag catcggcaat aacgctgccc gcctggtaaa aggcattccc ttcagggaat
    17701 gccactcata atccacagga gaaacgctgt atgaacaacg cactaggact ggttgaaaca
    17761 aaagggttag ttggcgcaat tgaagctgca gatgccatgg ttaaatccgc caacgtgcaa
    17821 ctggttggct atgaaaagat tggttcgggt ctgattacag tgatggtccg tggcgatgtt
    17881 ggcgcagtaa aagccgccgt tgacgcagga agcgccgccg caagcgccgt tggcgaagtg
    17941 aaatcctgcc atgtcatccc gcgcccacac agtgatgttg aggctatttt acctaaatcg
    18001 gcataacccc atttactaag gagcacagcg tgaagcaatc actgggatta cttgaagtca
    18061 gtggtctggc tctcgccatc agttgcgcag acgctatggc aaaagccgcg tccatcacgc
    18121 tggttggcct tgagaagacc aatggctcag gctggacgct catcaaaatc accggtgacg
    18181 tcgcctcggt taatgcggcg atcgcgacgg gagccagttt cgccggacag cgaaatggcc
    18241 ttgtcgcaca caaagtgatt gcccgcccgg gagacggcat tctgccccac acaactgaag
    18301 cattgaccgt gacggttgaa acaagcaaaa caccagcaac aaccgtcacc tcagagatta
    18361 tcagttgtaa tttgtgtctt gacccggcat gtcagcggca aaaaggtgag ccccgctcac
    18421 tttgcctgta cgccaataag cgaggggagt agcagtggat aaacaaacct tgcaggaaac
    18481 agtgaaccag attctggatg agatgcgcga gcgccctatc ccactgggcg tttccagtcg
    18541 ccatatacat ctttcaacag cggattattt gcgtcttttt cctgaacaac cgattagcga
    18601 gaaaaagccg ttaatgcaac ctggccaata cgctgcggaa caggttgtta cgctggaggg
    18661 acccaaaggc aaattgcaca acgttcgctt gttggggccg ctacgcaatg caagtcaggt
    18721 ggaaatttcc cggacggacg ccagaacgtt agggatcacc gccccgctac gcatgtcagg
    18781 cgatctcagc ggcactccgg ggattcgttt gataagccca tttgcggaac tggagatcac
    18841 atctggcgtt attgtcgcgc aacggcatat tcatatgtct ccgctggatg ccctgattta
    18901 tggcgttacg catggcgata aagtggcggt tgccattgaa ggtgatgagc gacgactgat
    18961 attcgaaaac gtcgctgttc gcgtctctcc ggatatgcgt cttgaaatgc atattgatac
    19021 cgatgaggcc aacgccgcag gcgctgatag ccctgacgtt tttgccacac tggtgtcagt
    19081 gccatgaata acatgctgat gcaacgtatt gttgacgaag tggtttttcg ccttaaacaa
    19141 cgtgcgggaa aaacactcgt gctgactgtt tttcaactgc gagacgccag cgttcaagag
    19201 agcgttcatc agtatgcgtc attacaaatc cgatatgtcg atctgccgct gctgcgacaa
    19261 cttgcggaga acgaaacgtc agatcgagca gcgattcaga tacacgaagc gttagcctgg
    19321 ggattgcaca ttcaattgtc gttgcagcgt catttcctga acgccattga attgaagact
    19381 ctcgcccgtc taccgctaag ctggtgcgac gaacaagggc aaccgatcta ccttcatcgt
    19441 gatcgacttt taagttatgc cgatatcgca cagttaagct cgggaatact ggttttacag
    19501 cggaaatgtt gtgtgaccgc actggctcgt gaagcggcaa ttacccgaaa tattcagtta
    19561 atcaggcagg agtaacttat gcatctggct cgagtcactg gcgcggtagt atccacgcaa
    19621 aaattacctt ctctgatcgg gaaaaaactg ctgctggttc gccggatcag tgctgacggc
    19681 gaactgcctc tcacgccatt aaccggcgac gaagttgccg ttgattcagt cggcgcaggc
    19741 gttggtgagt tggtactcct cagcagtggt tccagcgcca gacatgtgtt ttcaggccct
    19801 aacgaggcca tcgacctggc agtggtcggc attgtcgata cgctttcgcg ctaaggagct
    19861 aaaagatggc aatttatacc cgaacaggtg attcaggaag tacgtcttta tttaccggac
    19921 agcgcgtcag caaaactcat ctacgtgtcg aaacctatgg cacgctggat gaactgaacg
    19981 cgacactcag tctgtgttat tgcgccaccg ccatcgaaag ccatcgcatc ttactggaag
    20041 ccattcaaca acagatattt tggtttagcg ccgaactcgc cagtgaaagt gaacagccgt
    20101 cagcgcaaca gcgttacatc ggtacggaag aaattgctgc gctggaaaac gcgatcgata
    20161 gcgcaatgaa cgccgtcccc ccagttcaca gcttcattct gcctggtcga tgtgaagccg
    20221 cgagccgcat gcatttcgcc cgcacagtgg ctcgacgggc tgaaagacgt ctggttgaac
    20281 tgacaacgga aaccaccgtc cggaatgttt tgctgcacta tatcaaccga ctttccgatt
    20341 gtctgtatgc cctcgcccgc gtggaagaca acgttgctca tcaaaacctg atgattcagg
    20401 aaatcacaaa acgttatcac gaagccaacc acataccagc attgaaggaa cgcacaatgc
    20461 cgctcacttt tcaggatctt caccagctca ttcgtagtgc ggcaatgcgc gcggatgaac
    20521 tgcatattcc cgttgtcatc agcattgttg atgccaacgg aacagaaagt gttacctggc
    20581 ggatgcctga tgccctgttg gtcagcagtg aactggcacc gaaaaaagcc tggaccgccg
    20641 tggcgatgaa aacggcaacc cacaaacttg ctgataccgt gcaacccggc gcgccgcttt
    20701 acgggctcga aagccatatg caaggcaaag tcgtcacttt tggtggcggc ttccccctct
    20761 ggcgtgacgg aaaattgctt ggcgggcttg gcatcagcgg cggtagcgtt gaacaagaca
    20821 tggatattgc tcaaagcgca atggcggcaa ttaacgtggg agtaaaccaa tgaataactc
    20881 tgaattagaa acactgatcc gcaatattct gcgcgagcaa cttgtccccg ctacaccacg
    20941 atcgcaaaga aacgctattt tccagactgt tgatgaagcg gtctgcgcag cacaccaggc
    21001 ctttttacgc tttcaacagt gtccgcttaa aacccgtagc gccattatca gcgccattcg
    21061 cgaagaatta atgccccgcc ttaccgaact ggcagaagaa agcgccaaag aaacggggat
    21121 gggaaataaa gaagataagt acctaaaaaa caaagcggca cttgataaca cgccaggaat
    21181 tgaagattta accaccacag cactaaccgg tgatggtggc atggttttgt ttgagtactc
    21241 tccgtttggg gtgattggct cggtcacgcc aagcaccaac cccacggaaa ccatcattaa
    21301 caacagcatc agcatgctgg cagcaggtaa tagcgtctac ttcagtccac acccgggtgc
    21361 caaaaacgtt tcgttaaaac tgattaccac aatcgaagac atcgcgtttc gctgtagtgg
    21421 cattcataac ctgatcgtta ccgtatctga accgaccttt gctgccaccg aacagatgat
    21481 ggcgcaccct aaaatcagcg ttctggcgat taccggcggt ccggggatcg tcgccatggg
    21541 aatgaaaagt ggcaagaaag tgattggtgc cggagcgggt aaccctccgt gcatcgtcga
    21601 tgaaacggcg gatattgtga aagccgccga ggacatcatc aacggcgctt catttgatta
    21661 caacctgccg tgtattgctg aaaaaagcct gattgtcgtg gcatcagtgg cagattattt
    21721 aattcagcag atgcaaagtt ttggcgcact tcttcttaac tatgagcaaa ctgagaaatt
    21781 gcgtgcaatt tgcctgccag acggtagcgc caacaaaaaa ctggttggga aaagcccatc
    21841 agccttgctg gaagcggcag gcctcccttt gcccgcaaaa gcgccgcgcc tactgattgc
    21901 tgttgtcgac gccaatgata gttgggttac ttgtgaacaa ctgatgccaa tgctgcccat
    21961 tgtgaaggtc aacgattttg atagcgctct caccctggca ctgaaagtgg aagacggctt
    22021 gcaccacacg gcaattatgc actcgcagaa cgtctcacgg ctcaaccttg ccgcacgcgt
    22081 tatgcaaacc tcaatctttg tcaaaaacgg cccctcctgg gcgggtattg gcgtcggcgg
    22141 cgaaggcttt acgaccttta ccattgcaac accgaccgga gaagggacaa cgtcagcacg
    22201 gacttttgcc cgctcacgac gctgcgtgtt aaccagcggc ttttcaattc gctagagaaa
    22261 cggaaatgaa tactttttca ctgcagacgc gtttatacag cggaccggga agtctcaagg
    22321 taattagccg tttctctcat aaacacatct ggattatttg cgacgtcttt ctggcacgct
    22381 caccactcat taacacgttg catcaggcgc tgcccgacag taatcgtctt agtattttta
    22441 gcgaaataac gcccgaccca actatccaga ccgtggtgaa aggcattgcg caaatgcaga
    22501 ctttacgccc ggatgtggtt atcggttttg gcggcggttc tgccctggac gccgcaaaag
    22561 cgattgtctg gttcggtcgc cagtgtggca ttgaaattga aacctgcgta gccattccta
    22621 ccaccagcgg taccggttcc gaagtgacca gtgcttgtgt catcagcgat cctgagaaag
    22681 ggattaaata tccccttttc gataatgcac tttatccgga tatcgcgatc cttgatccat
    22741 ctctgatcgt cagcgtgcca ccggcgataa cggccaatac cgggatggat gttttaaccc
    22801 atgcgcttga agcttatgtt tctcctcgcg ccagcgactt cacggatgca ctggtcgaaa
    22861 aagccgtgca aattgttttt caatacctgc ccactgcagt gaaaaaaggc gattgtcttg
    22921 ccacccgtgg caaaatgcat aatgcatcga cgcttgcagg aatggccttt agccaggccg
    22981 gactcggttt aaaccatgca attgctcatc aactcggcgg acagtttcat ctgccgcatg
    23041 gactggcgaa tgcgctcctg ctcacggcag ttatccgctt taatgctggc gatccgcgcg
    23101 ctaccaaacg ctacgcccgg ctggcgaaaa cgtgccaatt atgcccggac aacgccaatg
    23161 acaccgccag cctgaatgca cttattcaac atattgagca gcttaaaaca acctgtaagc
    23221 tgccgactct tgccaatgcg ctgaaagaaa aaaaagcaga atggtcatta tgcataccag
    23281 atatggttca ggcggcactg gcggatgcaa cgttgcgtac caacccgcgt gcggctgatg
    23341 tctccgcgat tgcagaactg ctcgaggagt tgttatgaac gtcggattaa tctcctgcga
    23401 cgaccagacc atccgcgagc gagttcaagc agcaggtgtc gtgggtgctg gcggcgcagg
    23461 ttttccaacc catattaagc tacaagcgcg tgttgacact ttcctggtga atgcggcgga
    23521 atgcgaacca atgttgaaag tggatcaaca gttgatggcg cagcaagcca gccgcctgct
    23581 tcgcggtgtt cactatgcga tgaaagccac cggggccagt agcggaatca tcgcgctgaa
    23641 agagaaatat caacgggcaa taaacgcact cacaccactg ttaccaccgg atatccgcct
    23701 gcatattttg cccgacgttt acccagcagg agacgaagtg ctgaccatct ggatggcaac
    23761 tggtcgccgc gttccacccg ctgcattacc cgttagcgtc ggtgtggtag tcaataatgt
    23821 tcaaaccgta cttaatattg ccagagccgt cgagcaacag tatccagtga cgcgccgcac
    23881 actcaccgtt aatggtgcag tggcgaaacc aataacggta accgtcccca tcggcatgtc
    23941 tttacgtgaa gtcctggcgc tggcgggcgg cgcaacagtg gatgctcctg gttttatcaa
    24001 tggcgggccg atgatgggaa gtttgatcac atcgctcgac acaccagtca gcaaaaccac
    24061 tggcggcttg ctggttttac ccaactctca ttcactgatc caacgtcgat tgcagaatga
    24121 ccgcagcgta cttgccgtcg cgaaaactgt ttgtgagcaa tgccgcttgt gtaccgatct
    24181 ctgcccacgt catttaatcg gtcacgaact ggcaccccat ttattagtac atgcggttaa
    24241 ctaccaacag ctcgccacgc cgcaactgtt actgacagcg ctcacctgtt cagagtgcaa
    24301 cgtctgcgcc agtgtcgcct gtccggtagg aatttcacca atgcgcataa accgcctgct
    24361 taagcaggaa ttacgcgcac aaaatctccg ctacgaaggt gccttaaatc ctgcagatcc
    24421 catggcaaat tatcgactga ttcccgtaaa acggctggta accagacttg gcttaacccc
    24481 gtggtatcag gatgcgccat tgtctgaaca ggtgcctcag cccgaaaaag tgacgttatt
    24541 gttacgccag cacatcggtg ccagcgcaat tgcgtgtgta caaaaaggcg atcgcgtcgt
    24601 tcatgggcaa tgtgtaggtc aaattccaca cggcacatta ggtgccccca ttcatgccag
    24661 tattgatggc atggtcagcg atgtgacaga aaacgcgatt acgcttgtga gaggttaacc
    24721 atgtcgaaag cggtaggtat tttagaactt agcagtatcg cgaaagggat ggaaaccggc
    24781 gacgccatgc tgaaaagcgc caacgttgag ttactggtca gcaaaacgct atgccccgga
    24841 aaatttctgc tgatgctcgg tggcgatgtg ggcgcggtgc aacaggccat caacagcgga
    24901 gcgtcccttg ctggtgaaat gctggtcgac agtcttgtcc tgccgaatat tcatcccagc
    24961 ctcctttctg caatcagcgg acttaaccat gttgaacagc accgggctgt cggtgtagtc
    25021 gaaacctgga gtgtcgccgc ctgcattgac gctgccgatt gtgctgtcaa agctgcaaat
    25081 gtcattcttg ttcgcgtaca tatggctttt ggcattggcg gtaaatgcta catggtggtt
    25141 gctggcgata tttcagatgt taataacgcg gtctctgtcg ccagcgagcg tgcgggggaa
    25201 aaaggtttgt tggtctatcg gtcggtgatt gcccgtccac atgaagccat gtggcgtcag
    25261 atggtggagg gataatgaca actcagcaca caactgaacg gatgattcag gaatatgtcc
    25321 ctggtaagca ggtcacgctg gcacatgtca ttgctaatcc agggaaggat ctgtttaaga
    25381 aattgggttt accagacacc atttccgcta ttggtattct gacaattacc ccccagcgaa
    25441 gcatctatca tcgcatgcga tatcgccaca aaatcaggcg ctgtagagat cggttttctt
    25501 gatcgtttta ccggtgccgt agtgctgact ggtgatgttt ctgcgatcga atatgcactt
    25561 agccaggtta cccgtacgct gggtgaaatg atgcgcttta ccgcctgccc cattacgcgg
    25621 acctgacgtt atgcaacgta tgatgctcat cggccccagc cagtgcggaa aaacgtcact
    25681 cacgcaatgt ctgcgtggcg aaaatattca ttatcagaaa acgcaggcaa tcgtctggtc
    25741 cccgaccacc attgatacac caggcgaata cctcgaaaac cgcagcctct acagtgcact
    25801 gttggtcagt gcctgcgaag cggacattat cgcgctggtg cttaatgcgg acgcaccgtg
    25861 gtcacctttt tcgccaggct ttacctcgtc aatgaatcgc ccgatcattg gagtagtcac
    25921 caaagcagat ttagccagca tggaacaaat atctttggtg aaatgttggt tacgggaagc
    25981 cggagcgcac aacgtgttag taaccaatgc ggttaataac catggagtta cagaactttt
    26041 tgccttgctg catacagaag atgtctgtcg ctaatttcga taggttatca gatgggaatg
    26101 atgactgtga tacacagaaa agaatcaatg agcaatgagg gttgccgggc aaccctcatt
    26161 gaataaaaca ggaattactt cgcttcgccg tttttcattt cgcccatagc tttcagcttt
    26221 ttggagatat cgcggcgttc tttggagagc tcggcatttt tgatgatgta atcgtcaacg
    26281 cgatcttcat agtcaccttt catgctagcg atgatgccct ggattgcttc aacgctcatc
    26341 cccggcttga tgtaatcgct caggttgtcc agcagcagga cgcgcttctg gttgtcacgg
    26401 atctttttct caacgtcctg aatttcacgt tgcagtttgt tcttacgacg gaacagacga
    26461 acaaattcca gtacgtcctg gaatgaaggc ttggtagttt ccatttttat acccctgata
    26521 atgtgagagt cggattcgtt taagcaaccg ctattcgtta gggccaacat tactcatagc
    26581 tgccgcagat gacaatgctt ttatcccttt tcactatcat accctttatc cttgctgaat
    26641 cgaagcagca gcaagatgat tctgaagttc aggaattatt tgcgcaaggc ccggcaaatc
    26701 agattcgata gcaactcaag ctgatgtgcc gtctccatgt tcagccacac ataaccatag
    26761 attggtgttt ccacaacctt caggtcatgg tggttattga gcaactgacg cagctcttcc
    26821 acagcatcgt tcaatttttc cgtattggca aaaaccggct gcggattacc ttcgtacagc
    26881 gcatgaacaa ggctcagcag tatttgctgc atcatgtgct gggtgtcacg aagtttttgc
    26941 gcgttcaata acacgaaatg gctggggcgc gtggcccagt acgcattgat ttgcaactcc
    27001 agcatacaaa ccagattgcg attaatggtc tggatacctt catatatcga ttttggaata
    27061 cgagtttctt tgctggcggg cgcaatcagc ccacgcattt tcacggcatc ggtcaagagt
    27121 ttttgtagat ggctttccag acgtgggcgt tcgagtaagt tcggtgagaa tgcagattga
    27181 taaacccgat tatactcggt cagacttttc gccagttgaa tgcgccagtg gatgaacgcc
    27241 cgttgtggcc agataccggt aaacaacatt gcgagtaaag agccgaggat cacatcgccg
    27301 cttcgccata acgccgtatc aatttcacct gtcggggaac ccacaacaat tgccagcgtc
    27361 accccaatca ataatccttg atacggtttc ttgcccagtg ccagccaacc gcaaagaaac
    27421 atagccgccg cgcaccagac taacatcagc ggtaacgaaa ttaactccag ttgcagagcg
    27481 ataagaccta atatcgaacc caacaccgta ccgccaatac gttcgaaggc tcgagggaca
    27541 acgttacccc agaacgagat tggccccata atcaccacca tggtgaccag cggccaggtg
    27601 ctttccggga tagtaaacag gcggataatg agaaaagtga gcaggaatgc cagcgcgacc
    27661 cgagtaccat gcacaatgcg gtaatggcgg tatacccgga tttcaaacgg gcttaatgac
    27721 ttatcggcac gcacgcagaa actccgtaat gagattaaaa gcgctcataa ctcatctgtc
    27781 ggccagaagg cgcgcctctg aattatccga cggacaaacc gggaacagtg taatttcccg
    27841 gcaggagcga aaaaattgaa aggcgcatct tactcttttc gctttcaaaa aaaagagtgg
    27901 tcatcgcgtt aacacaccgc cctgagatga attagtgatg ttttgactgc accgcaacat
    27961 acatttcgat atcccagtac ccatcttccg cgccattgtt caaataaacc tcgaagcagg
    28021 gcttgggtaa catttcataa gcactgtcct gcaagagact attaaagaac tgataccagg
    28081 gtttagcaaa atcatcaccg actacacgag ctaccgccac cgcatactga ccacctgtaa
    28141 tttctgtcag aatgacgccc tcactgtttt cgggaagcgt aaagtaaccc ggcaccgtca
    28201 cgacggtgtc gcagcgtaat ttttcggcgg gtgtttcatc tggattgtcg taatagacag
    28261 caacccactc cttcggcaca atatttttgc tatctaccca catcatcaac tgctcaaagc
    28321 ctttctttac cgtctgttcc cacgggccaa cgagatggaa acctgcaacg gtacgtttct
    28381 cttcctgctt aatctcgtag ttcatgacgc ctccattaac actgttttta tatacagtat
    28441 agttgcaata ttaaaaccac aaggaatgag tgttgattat gcgagcagac tcgcactcct
    28501 gccagtctgc tgcaaaagaa aggtcaggcc ttatggtgga aataatcact caggcgagaa
    28561 aacatgctgc cttccccaac agattccagg gtaaccagcg gccagtgcgc cacctgttta
    28621 tcacggtcgt aaagttcaat ttcccctacc cgctgatggg cgctaattgg cgcggtgagt
    28681 tctttaccat caagggtata tttggctttg atatgtggaa tttcggcttt cggtagtacc
    28741 atccagaact cttgttctgt tcccagggcg atattttctt tatcgccata ccagatgcgt
    28801 tccgtaccga cctttttccc acggtgcaaa atttgcaccg tagtaaagtt ttgttgcccc
    28861 caacgcagta attttcttgc ctcttcctca cgaccttttg cactgtcagc ccccattacc
    28921 actgcaatga gacgacgctg cccatctaca gccgaagcaa tgaggttaaa accggcacca
    28981 gaagtatgac ccgttttcag gccgtcaaca ttcatggttt tatcccacaa caacccgtta
    29041 cggttttgct gggtgatacc gttccaggtg agacttttct cactgtacat atgataaaac
    29101 tcgggctcgc cgtggatgat agcgcgagaa agcacagcta aatcataagc cgagctatgc
    29161 tggccaggtg catccagacc atgcactgtt tcaaaatgcg tatccttgag atgcagcttc
    29221 tcggcatagt tgttcatcat ttcaacaaac tgccgttgcc caccggcaat atagtcagcc
    29281 agagcaacac aagcgtcatt tccggaatcc acaattaaac cacggcttaa atcacgtacc
    29341 gatacgcgat cgccctcttt caaaaacatc agtgaagaac cgacaaacac cggattatct
    29401 ttcgcccacg catcgcgccc cacggtgaca atatcgtctg gcgtaatgcg atgactatcg
    29461 atagcgcgat ccacgacata acccgtcatc agctttgtca ggctggcggg attgcgctgt
    29521 tgatgctcat tacccgcggt gaggatctga ccggtggtgt aatccatcaa tacccaggac
    29581 ccggcatgaa tctctggagg ctgaggtgaa aaaggaatgt tttccgccgc aaaaccagac
    29641 gataagttaa aaacgaacaa agaagcagca ataataagac ggcgtttcaa cagcaaaccc
    29701 tcaggagttt caaatagctg ttctttttac ggaaatactt atgaactggc tggaataaag
    29761 tgcaagaaaa tgtgactacc ctctcatttt tatctgacat gatctgttgc cactcgctgc
    29821 caaattgtgg cgctaaagct gattagcacg gtgatatttg atactctggc agacagcaga
    29881 aataacggat ttaacctaat gatgaatgac ggtaagcaac aatctacctt tttgtttcac
    29941 gattacgaaa cctttggcac gcaccccgcg ttagatcgcc ctgcacagtt cgcagccatt
    30001 cgcaccgata gcgaattcaa tgtcatcggc gaacccgaag tcttttactg caagcccgcg
    30061 gatgactatt taccccagcc tggagcagta ttaattaccg gtattacccc gcaggaagca
    30121 cgggcgaaag gagaaaacga agccgcgttt gccgcccgta ttcactcgct ttttaccgta
    30181 ccgaagacct gtattctggg ctacaacaat gtgcgtttcg acgacgaagt cacacgcaac
    30241 gttttttatc gtaatttcta cgatccttac gcctggagct ggcagcatga taactcgcgc
    30301 tgggatttgt tggatgttat gcgtgcctgt tatgccctgc gcccggaagg aataaactgg
    30361 cctgaaaatg atgacggtct accgagcttt cgccttgagc atttaaccaa agcgaatggt
    30421 attgaacata gcaacgccca cgatgcgatg gctgatgtgt acgccactat tgcgatggca
    30481 aagctggtaa aaacgcgtca gccacgcctg tttgattatc tctttaccca tcgtaataaa
    30541 cacaaactga tggcgttgat tgatgttccg cagatgaaac ccctggtgca cgtttccgga
    30601 atgtttggag catggcgcgg caataccagc tgggtggcac cgctggcgtg gcatcctgaa
    30661 aatcgcaatg ccgtaattat ggtggatttg gcaggagaca tttcgccatt actggaactg
    30721 gatagcgaca cattgcgcga gcgtttatat accgcaaaaa ccgatcttgg cgataacgcc
    30781 gccgttccgg ttaagctggt gcacatcaat aaatgtccgg tgctggccca ggcgaatacg
    30841 ctacgcccgg aagatgccga ccgactggga attaatcgtc agcattgcct cgataacctg
    30901 aaaattctgc gtgaaaatcc gcaagtgcgt gaaaaagtgg tggcgatatt cgcggaagcc
    30961 gaaccgttta cgccttcaga taacgtggat gcacagcttt ataacggctt tttcagtgac
    31021 gccgatcgcg cagcaatgaa aattgtgctg gaaaccgagc cgcgtaattt accggcactg
    31081 gatatcactt ttgttgataa acgaattgaa aaactgttgt tcaattatcg ggcacgcaac
    31141 ttcccgggga cgctggatta tgccgagcag caacgctggc tggagcaccg ccgccaggtc
    31201 ttcacgcccg agtttttaca aggttatgca gatgaattgc agatgctggc acaacaatat
    31261 gccgataaca aagaaaaagt ggcgctgtta aaagcgcttt ggcagtacgc ggaagagatc
    31321 gtctagtttg aagcctccgc cgctgatacg gcggaggaca atgttgggtc aggctttttg
    31381 aacggtgatg ctccacgcag catcgccaat ctgctgataa tcggtgatgg catgtccttc
    31441 ttctgccgcc cattgtggga ttgcttctgt tgcctgggtg caatcaaact caattaccaa
    31501 ctcatcgccg ctggccattc ctgccagtgc tgcttttgct tctatgagtg gaaacggaca
    31561 gacctgcgtc accacatcca gctttttaat cgccatcttt acaccttact taatttgccg
    31621 cagcagttgc cagtctcgcc ttacgctgcg gtcgaacata cacaagccag gacgcagtcc
    31681 agactccgag aatcataaat acaaggccaa tccagccctg ccaggtcatc attgcagtca
    31741 taaccagtcc attaccgata gagcaaccac ctgcaatact ggcaccgaag cccatcagta
    31801 caccacctaa cccgctacgt aatgttgttt gtgcatcagc tgcgcgaacg cggaactcac
    31861 ggctcgcttt ggctgcaata aatgacccca cgaagatccc taacaccagg aaaacgcccc
    31921 agttaatgta tttcacgtca cccgcgacca gaaattgcag aatattggcc gttggggaag
    31981 tgattcctaa cccaaacatg cgtccggttg cttcactcag gggccaggct aaaagcgcaa
    32041 tcaaaccgat gagtacagca gtgacaaagg gatgccagcg tttttcaaac agaatatgag
    32101 cgatcccggt tcggcgcggc ggtaaggtcg cgactttgag ttttggcttc ttcaactctt
    32161 tcatcaccac ccagagcgtt atcaccagca aaacggcaac caacggccac acagacaaat
    32221 taaaagtctc agcaatagag ttatgttcag tactgtagtg ctgcaaggtt tgatttaaac
    32281 cactggcatg tggagaacgc atcaccgcac tcatcaccat ataagtgaaa agcgcgatcc
    32341 aactgccgat caatccctca cccgcgcgat accaggtccc ggtggcacat ccacccgcca
    32401 gaacaattcc cagcccaaag atatacccac ctataacagt accaagccac gggaacgccc
    32461 cggcttcgta agtcagtagg cccgcctgaa ttaacgcaaa aacccccaca ctttgtaccg
    32521 aaattgcaat cagcaaggcg taaaacatgc gattattttt cgcgagatac atatcgcgaa
    32581 aaccacctgt caggcaaaaa cgcccacgct gcatgacaaa tcccagcaga gcaccacaaa
    32641 ttagcccgct taatatcatt gaaaacataa attaattaac cagatgaatg ttaataggga
    32701 aattattcat gactggtgga atgcagacca ataaccaaat tctctaaatt agaacaaatg
    32761 gttattaatg aggatgtcga taaaaaagaa taaaaaaacc ggagccaaat attcattcga
    32821 ctccggttta ttattacaaa agatgattac gctacgtctt cgtactgcgg aaccgggtta
    32881 cggaaactct tggtgacgca agccaggtaa atcagaccga tagccgccca gatcagaccc
    32941 agaaccattg agctttcttc caggttaacc cacagcgcac caacggtcag cgcaccacac
    33001 atcggcagga acagatactg gaagtgatct ttcagcgtct tattacgctt ctcacggatc
    33061 cagaactgcg agatgaccga caggttaacg aaggtgaacg ccaccaacgc accaaagtta
    33121 atcagcgctg tagccattac caggtcgaag ttgattgcca gcagggcaat cgcgccaacc
    33181 aggatgatgt tcatcgccgg agtacgccat ttcgggtgta cataaccgaa gaagcttttc
    33241 gggaatacgc cgtcacgacc cattacgtac atcagacgcg ctacgcctgc atgcgccgcc
    33301 ataccggacg ccagtacggt aatggtggag aagatcagcg cgccaacctg gaacgcttta
    33361 cccgcaacgt acagcatgat ttcaggctgt gacgcatccg gatctttaaa gcgagagata
    33421 tccgggaagt acagctgcag gaagtaagtt gcaaagatga agatcatgcc gccaatcagc
    33481 gcggtcagga aaatcgcacg cgggatcacg cgctctgcat ctttggtttc ttccgacagg
    33541 ttgctgatgc cgtcaaagcc ggtaaaggag aagcacagga ttgtcgcccc ggtaatcatc
    33601 gggataacat gcgcatcgcc agaccagaac ggacgagtgc tcgccagcgt accagcgcct
    33661 tcaccttcaa atacgccata aacaaccatg cccagaatca ccgcgatcag cactacctgc
    33721 aacacgacga ttacagtgtt gaagttcgct acggatttca gactacgcag gttaaaggcg
    33781 gtcataaagg ccaccagcgc caccacaaac atccacgatg ggatggaagg caccagagct
    33841 tcaaaataga ttttcgccag cagaatgttg atcatcggcg cgaacagata gtcgagcaga
    33901 gaagaccaac ccaccataaa gccgacagtc gggctaatgg atttctgggc gtaagtgtat
    33961 gcagagccag cagaaggata gcggcgaacc agcttcccgt agctcagagc cgtaaacagg
    34021 atcgcaatca atgcgaacgc ataggctgtc ggcacatgac catccgtgag gcctgaaacg
    34081 ataccaaaag tatcaaacag cgtcatcggc tgcatatagg caagacccat cattacaacc
    34141 ggaactaacg taagcgtttt acgcaattcc acgcgagagg tgtttggagt aacgttatgc
    34201 gacatggtca ttctccttta cggtgataac cgtcgcgtaa gcaaaaaatt gccccatttt
    34261 tttggattcc tcagcgacaa caactgtcga tttttagtaa atatctatcc ggtacgaagc
    34321 ccggcctctt ggtatgaatg attggtttga agcaaaaaat aaccgacgct gatgaaacgt
    34381 cggtttttag tcattttttg acagcggcgc attgtgccta aatggggggg aaatgacaag
    34441 agaatgagag gcttgtcata ataatttttc tttaaatggc tgattttccg tcatcagatt
    34501 ttgcataaac accggcgatc gcactatttg ctaaaatttc atcccgccac cagccgctgg
    34561 caagccctgc ggtctgttca ttccagccaa tccagacggg ttcaaagaga gtttgggcga
    34621 ccactttctt ctcgattaac gcgccactat cgagaaaccg ttgcgccaga taacggggta
    34681 aatagccgca gccgaggccg ctaatttgca gctccagctt ggttttaaaa tcgaaaacgg
    34741 taatggcttc ttgttcgtcg agaagttgtg aggccgtaga agcgcctgcc tgcgcagtat
    34801 cccccaccac aatcgcccga tatcgcttaa taatacgacg gtttaacggc tcttcttcca
    34861 gagctaacgg atgatgaggc gcgaccgcaa agacttgttc cagatcgccc aaccgtgaaa
    34921 aaccaaactc actggaagat ggtggctcat gcatcgcgcc aacaataata tccgctcgcc
    34981 cctgagtcag ggcatcccag gaaccaccga gtacgccatt gataaacttc aggcgcgtca
    35041 cgctatgatg ttgataaaaa gcttcaatca gtggcgcaag cagagaaaaa ggaaacgtgt
    35101 cgtccacgcc aatcaccagc tcgttttccc agccttcatg cagtttgatc gcctgctttt
    35161 ccagttcccg cacggtatgc agaacttccc gccctttctc taataacatt tttccggtgc
    35221 gggtgaattt agcgcggtgt ccgctacgat caagcaattg aatattaagg tcgctttcca
    35281 gcttgtgaac ggtgtaactc agagcggacg gcgtcttgta gagtttggcc gacgccgccg
    35341 caaaactgcc ttctttttct aaggcatcga ggatcatcaa cacatccagc agtggtttca
    35401 tacacgcccc cttacagtgc atgcggcgtc ctgctggcgt gatcgtttac tccagcggca
    35461 ttaccagcgg atcgggatac tggtattcaa atcccagttc attacaaatc cgactgccat
    35521 caataatctt acctttgccg ctgtccagac tatttctgaa ctgcggaggt tccagcccca
    35581 gtaaacgggc catctgcgga tagaaaacat tacgcgcagg gtgagctggc gcacatatat
    35641 tatagatgtg tccgccttta ggtgcctgta acaacagagt gatagcgcca ataacatctt
    35701 ctaaatggac taaattaaca ccatgttcac catcaggcgc ggtttttccg gcaaagaagc
    35761 gtccgggatg acgtcccggt ccgacaagac ccgcaagacg cagaatatcg accgaagtac
    35821 cgggtaaatt gtgcagccag tcttcgagtt cttctaatac tcgtccactg ttggttaccg
    35881 gattacgcgg ggtggtttct ttcaccgtgc cttgcgcgtc gccatagaca gatgttgagc
    35941 tggtaaaaat aattcgggga atacgatggg ccagcgcgct atccactaac tcttgtaccg
    36001 cttgtaaata gaactcatcg ccggagccgc tacgacgtgc cggaagcgta atgaccagtg
    36061 catcggcatc catcagggca tccagatcgt cagaatcgca aactaactca ggctccatgc
    36121 gaagcagata gctatcaatg ccactcattc gggccgcttc gacaccatct tgtgtggttt
    36181 tactcccggt gacttgccag cctcgcgctg aaagtgacat cgccagcggc atgcctaacc
    36241 accctaaccc gacaattgcg acctttttca tccgttatct cctgatacac cttagatcta
    36301 taaggctacg ctagcgtatc aaaactgaca attcattcta tgaatgaatc tgttcaataa
    36361 tgataacgac atgctgcaat gagcagtgaa tcgtcggtaa ccgcgtatac cagacggtgc
    36421 tcctctgtaa tgcgtcggga ccagaaacct gacaaattat gtttcagggg ttctggcttc
    36481 cccttacctt caaatggcgt tctgcgggta tctttgataa gttcattgat ctttttaaca
    36541 attcgcttat ctgtttcctg ccagtacaga taatcatccc atgattcctc agaccagatt
    36601 agtttcactc aataatgtcc ttttctgttc ctttgcctga tttcaggcta tcgattgagt
    36661 ccatcaatct ccgggcgtta gcgggggaac gcagtagata agccgtctct tccagcgagt
    36721 tgtattcttc gagtgacatc agaacacaag cctctccatt ctgacgagta ataaggatcg
    36781 gggcatgatc ttcaacggct ttcatcattg ttgccgacaa attctgacgc gcttcgctgt
    36841 agctaattgt acgcatgtca atctcctctt ttgtacagtt cattgtacaa tgatgggcgt
    36901 taattaacta tttattaatt agtttgtaga tcaagatatt gtcagtgaga cgaaaaatcc
    36961 aggcttcgct attttgagta ccgtcaggtt agaggacagt cctcttagcc ccctcctttc
    37021 cccgctcatt cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct
    37081 aacgtgaaag tggtttaggt taaaagacat cagttgaata aacattcaca gagactttta
    37141 tgacacgcgt tcaatttaaa caccaccatc atcaccatca tcctgactag tctttcaggc
    37201 gatgtgtgct ggaagacatt cagatcttcc agtggtgcat gaacgcatga gaaagccccc
    37261 ggaagatcac cttccggggg cttttttatt gcgcggttga taacggttca gacaggttta
    37321 aagaggaata acaaaatgat agacaacact cgtttacgca tagctatgca gaaatccggc
    37381 cgtttaagtg atgactcacg cgaattgctg gcgcgctgtg gcattaaaat taatcttcac
    37441 acccagcgcc tgatcgcgat ggcagaaaac atgccgattg atattctgcg cgtgcgtgac
    37501 gacgacattc ccggtctggt aatggatggc gtggtagacc ttgggattat cggcgaaaac
    37561 gtgctggaag aagagctgct taaccgccgc gcccagggtg aagatccacg ctactttacc
    37621 ctgcgtcgtc tggatttcgg cggctgtcgt ctttcgctgg caacgccggt tgatgaagcc
    37681 tgggacggtc cgctctcctt aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc
    37741 aagcgttatc tcgaccagaa aggcatctct tttaaatcct gcttactgaa cggttctgtt
    37801 gaagtcgccc cgcgtgccgg actggcggat gcgatttgcg atctggtttc caccggtgcc
    37861 acgctggaag ctaacggcct gcgcgaagtc gaagttatct atcgctcgaa agcctgcctg
    37921 attcaacgcg atggcgaaat ggaagaatcc aaacagcaac tgatcgacaa actgctgacc
    37981 cgtattcagg gtgtgatcca ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc
    38041 gaacgtctgg atgaagtcat cgccctgctg ccaggtgccg aacgcccaac tattctgccg
    38101 ctggcgggtg accaacagcg cgtagcgatg cacatggtca gcagcgaaac cctgttctgg
    38161 gaaaccatgg aaaaactgaa agcgctgggt gccagttcaa ttctggtgct gccgattgag
    38221 aagatgatgg agtgatcgcc atgagcttta acacaatcat tgactggaat agctgtactg
    38281 cggagcaaca acgccagctg ttaatgcgcc cggcgatttc cgcctctgaa agcattaccc
    38341 gcactgttaa cgatattctc gataacgtga aagcacgcgg cgatgaggcc ctgcgggaat
    38401 acagcgcgaa gtttgataaa accacggtta ccgcgctgaa ggtgtctgca gaggagatcg
    38461 ccgccgccag cgaacgcctg agcgacgagc taaaacaggc gatggcggtg gcagtaaaga
    38521 atattgaaac cttccacact gcgcaaaaac tgccgccggt agatgtagaa acgcagccag
    38581 gcgtgcgttg ccagcaggtc acgcgtccgg tagcttcagt tgggttgtat attcctggcg
    38641 gctccgcccc gctcttctca acggtattaa tgctggcgac tccggcgcgt attgcgggct
    38701 gtaaaaaagt ggtgctgtgc tcaccgccgc cgattgctga tgagatcctt tatgcggcgc
    38761 agttgtgcgg tgtacaggac gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg
    38821 cgtttggtac agaatctgtg ccgaaagtgg acaaaatctt cgggccgggc aacgcctttg
    38881 tcaccgaagc gaaacgtcag gtgagccagc gtctggacgg tgcggcgatc gatatgcccg
    38941 ccggtccgtc ggaagtgctg gtgattgccg acagcggcgc aacgccggat ttcgtggcct
    39001 ctgatttgct ctcccaggca gaacacggcc cggactctca ggtgatttta ctgacgcccg
    39061 atgctgatat ggcgcgtcgt gttgccgaag ctgtcgaacg ccagctggcg gtactgccga
    39121 gagctgaaac cgcccgtcag gcactgagcg ccagccgcct gattgtgacc aacgatttag
    39181 cgcagtgcgt ggagatctcc aaccagtacg gcccggagca cctgatcatc cagacccgca
    39241 acgcccgcga tctggtcgat ggcatcacca gcgccggttc ggtatttctt ggtgactggt
    39301 caccggaatc ggcaggtgat tacgcctccg gcaccaacca cgtactgccg acttacggtt
    39361 ataccgccac ctgttccagc ctcgggctgg cagatttcca gaagcgtatg accgtgcagg
    39421 aactgtcgaa agaaggcttc tccgcgctgg cttcaaccat tgaaacactg gccgccgccg
    39481 agcgcctgac cgcccacaaa aatgccgtta ctttgcgtgt taacgccctt aaggagcaag
    39541 catgagcacc gtgactatca ccgatttagc gcgtgaaaac gtccgcaacc tgacgccgta
    39601 tcagtcggcg cgtcgtctgg gcggtaatgg cgacgtctgg ctgaacgcca acgaatatcc
    39661 cacagccgtg gagtttcagc ttactcagca aacgctcaac cgctacccgg aatgccagcc
    39721 gaaagtggtg attgaaaatt acgcgcaata tgcaggcgta aaaccggagc aggtgctggt
    39781 cagccgtggc gcggacgaag gtattgaact gctgattcgc gctttttgcg aaccgggtaa
    39841 agacgccatc ctctactgcc cgccaacgta cggcatgtac agcgtcagcg ccgaaacgat
    39901 tggcgtcgag tgccgcacag tgccgacgct ggacaactgg caactggact tacaaggcat
    39961 ttccgacaag ctggatggcg taaaagtggt ctatgtttgc agccccaaca acccgaccgg
    40021 gcaactgatc aatccgcagg attttcgcac cctgctggag ttaacccgcg gtaaggcgat
    40081 tgtggttgcc gatgaagcct atatagagtt ttgcccgcag gcatcgctgg ctggctggct
    40141 ggcggaatat ccgcacctgg ctattttgcg cacactgtcg aaagcttttg ctctggcggg
    40201 gcttcgttgc ggatttacgc tggcaaacga agaagtcatc aacctgctga tgaaagtgat
    40261 cgccccctac ccgctctcga cgccggttgc cgacattgcg gcccaggcgt taagcccgca
    40321 ggggatcgtc gccatgcgtg agcgggtggc gcaaattatt gcagaacgcg aatacctgat
    40381 tgccgcactg aaagagatcc cctgcgtgga gcaggttttt gactctgaaa ccaactacat
    40441 tctggcgcgc tttaaagcct ccagcgcagt gtttaactct ttgtgggatc agggcattat
    40501 cttacgtgat cagaataaac aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac
    40561 ccgtgaagaa agccagcgcg tcattgacgc cttacgtgcg gagcaagttt gatgagtcag
    40621 aagtatcttt ttatcgatcg cgatggaacc ctgattagcg aaccgccgag tgattttcag
    40681 gtggaccgtt ttgacaaact cgcctttgaa ccgggcgtga ttccggagct gctgaagctg
    40741 caaaaagcgg gctacaaact ggtgatgatc actaatcagg atggtctggg aacacaaagt
    40801 ttcccgcagg cggattttga tggcccgcac aacctgatga tgcagatctt cacctcgcaa
    40861 ggcgtgcagt ttgatgaagt gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc
    40921 cgtaagccga aagtaaaact ggtggagcgt tatctcgctg agcaagcgat ggatcgcgct
    40981 aacagttatg tgattggcga tcgcgcgacc gacattcaac tggcggaaaa catgggcatt
    41041 aatggtttac gctacgaccg cgaaatcctg aactggccga tgattggcga gcaactcact
    41101 aaacgagacc gttacgccca tgtagtgcgc aacaccaaag agacgcaaat tgacgtccag
    41161 gtgtggctgg atcgcgaagg tggcagcaag attaacaccg gcgttggctt ctttgatcat
    41221 atgctggatc agatcgccac ccacggcggt ttccgcatgg aaatcaacgt caaaggcgac
    41281 ctctatatcg acgatcacca caccgtcgaa gataccggcc tggcgctggg cgaagcgtta
    41341 aaaattgctc ttggcgacaa acgcggtatt tgccgcttcg gttttgtgct gccgatggac
    41401 gaatgccttg cccgctgcgc gctggatatc tctggtcgcc cgcacctgga atataaagct
    41461 gaatttacct accagcgcgt gggcgatctc agcactgaaa tgatcgagca cttcttccgt
    41521 tcgctctcat acaccatggg cgtgacgcta cacctgaaaa ccaaaggtaa aaacgatcat
    41581 caccgtgtag agagcctgtt caaagccttt ggtcgcaccc tgcgccaggc catccgcgtg
    41641 gaaggcgaca ccctgccctc gtcaaaagga gtgctgtaat gaacgtggtg atccttgata
    41701 ccggctgcgc caatctgaac tcggtaaagt ctgccattgc gcgccacggt tatgaaccaa
    41761 aagtcagccg tgacccagac gtcgtgttgc tggccgataa actattttta cccggcgttg
    41821 gcaccgcgca agcagcgatg gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag
    41881 cctgtaccca accggtgctg ggcatctgct tagggatgca actgctgggg cggcgcagcg
    41941 aagagagcaa cggcgtcgat ttgctgggca tcatcgacga agacgtgccg aaaatgaccg
    42001 actttggtct gccgctgcca catatgggct ggaaccgcgt ttacccgcag gcaggcaacc
    42061 gcctgtttca ggggattgaa gacggcgcgt acttttactt tgttcacagt tacgcaatgc
    42121 cggtcaatcc gtggaccatc gcccagtgta attacggcga accgttcacc gcggcggtac
    42181 aaaaagataa cttctacggc gtgcagttcc acccggagcg ttctggtgcc gctggcgcta
    42241 agttgctgaa aaacttcctg gagatgtgat gattattccg gcattagatt taatcgacgg
    42301 caccgtggtg cgtctccatc agggcgatta cggcaaacaa cgtgattacg gtaacgaccc
    42361 gctgccgcgc ttacaggatt acgccgcaca aggtgccgaa gtgttgcatc tggtggatct
    42421 gaccggggca aaagatccgg ctaaacgtca aatcccgctg attaaaaccc tggtggcggg
    42481 agttaacgtt ccggtgcagg ttggcggcgg cgtgcgtagc gaagatgacg tggcggcgtt
    42541 actggaagcg ggcgttgcgc gtgtggtggt cggttccacc gcggtgaaat caccagaaat
    42601 ggtgaaaggc tggtttgaac gtttcggtgc cgatgcctta gtgctggcgc tggatgtccg
    42661 tattgacgag caaggcaaca agcaggtggc agtgagcggc tggcaagaga actcgggcgt
    42721 gtcgctggaa caactggtgg aaacctatct gcccgtcggc ctgaaacatg tgctgtgtac
    42781 cgatatttca cgcgacggca cgctggcagg ttctaacgtc tctttatatg aagaagtgtg
    42841 cgccagatat ccgcaggtgg cgtttcagtc ctccggcggt attggcgaca ttgatgatgt
    42901 tgctgccctg cgtggcactg gcgtgcgcgg cgtaatagtt ggtcgggcat tactggaagg
    42961 taaattcacc gtgaaggagg ccatcgcatg ctggcaaaac gcataattcc atgtctcgac
    43021 gttcgtgatg gtcaggtggt aaaaggcgta cagtttcgca accatgaaat cattggcgat
    43081 atcgtgccgc tggcaaaacg ctacgcagag gaaggtgcag acgaactggt gttctacgat
    43141 atcaccgctt ccagcgatgg ccgtgtggta gataaaagct gggtatctcg cgtggcggaa
    43201 gtgatcgaca ttccgttttg tgtggcgggt gggattaagt ctctggatga cgccgccaaa
    43261 attctttcct ttggcgcgga taaaatttcc atcaactctc ctgcgctggc ggatccgacg
    43321 ttaattactc gcctggcgga tcgctttggc gtgcagtgta ttgtggtcgg cattgatacc
    43381 tggtacgacg ctgaaaccgc taaatatcat gtgaatcaat ataccggcga tgaaacccgc
    43441 acccgcgtca ctcagtggga aacgctcgac tgggtagagg aagtgcaaaa acgcggtgcc
    43501 ggagaaatcg tcctcaacat gatgaatcag gacggcgtgc gtaacggtta cgacctcgaa
    43561 cagctgaaaa aagtgcgtga agtttgccac gtcccgctga ttgcctccgg tggcgcgggc
    43621 accatggaac acttcctcga agccttccgc gatgccgacg ttgacggcgc gctggcggct
    43681 tccgtattcc acaaacaaat aatcaatatt ggtgaattaa aagcgtacct ggcaacacag
    43741 ggcgtggaga tcaggatatg ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa
    43801 ccgacggact gatgccggtg attgtgcaac acgcggtatc cggcgaagtg ttaatgctgg
    43861 gctatatgaa cccggaagcc ttagacaaaa ccatcgaaag cggcaaagtc accttcttct
    43921 cgcgcactaa acagcgactg tggaccaaag gcgaaacgtc gggcaatttc ctcaacgtag
    43981 taaatattgc gccggactgc gacaacgaca cgctactggt actggcgaat cctatcggac
    44041 caacctgcca caaaggcacc agcagctgct tcggcgacac cgctcaccaa tggctgttcc
    44101 tgtatcaact ggaacaactg ctcgccgaac gcaaatctgc cgacccggaa acctcttaca
    44161 ctgccaaact gtatgccagc ggcactaaac gcattgcgca gaaagtaggt gaagaagggg
    44221 tggaaaccgc actggcagcg acggtacatg accgctttga gctgaccaac gaggcgtctg
    44281 atttgatgta tcacctgctg gtgctgttgc aggatcagga tctggactta acgacggtga
    44341 ttgagaacct gcgtaaacgg catcagtgag ttgcggggta agcggatgcg atattgttgt
    44401 cgcatccggc aaaaaaaacg ggcaaggtgt cacccccctg ccctttttct ttaaaactgc
    44461 aaagattact tcgcgttgta attacgcaga gcgttacgcc ccaacacaat ccccgcgcca
    44521 accatgccgc ccagcagcac tgccagaatc aatgtaattg cctttttcgg actatcgcga
    44581 cgaataggta acgtcggttt cattacatag cggtaagcat gaatatcaag atcatcaacc
    44641 tttaagtttt cgatatccag caggttttga cgtgtctgat agtagtttgg tgagaacact
    44701 aacggacgag tcgcctcatg cttaatcatc gactccagag cttcgctccc caaaaggaac
    44761 aacgtatctt gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac ctgcgcctga
    44821 ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg ctcctgcgcg
    44881 actacttcct gcgttcttaa agagtcctgc aagtttttcc gtcccagagc aatgttgtct
    44941 ttgagatcct tttctagctc ttgattcact ttttcatcaa cttgctgaat gtattgggcc
    45001 aacttcattt gtgcgccctc tgctgtttgc ccaacataag aaacagtcaa tggtaattgc
    45061 tggttcttaa cagaaggttc gatggtaagt ttctctggct cttcctgatt atccagcgtt
    45121 tctgctaatg cagagaaggc agaactgaag cgaccaatta acgtctcctg caaatccgat
    45181 actttcggtg cagcctgacc atagataaca ttcatagcat tgttatagcc agcaatttgc
    45241 cccacgtcgg gctgagtgat aattgctgtt gacgtccatt tctccttcgc tactgccaaa
    45301 tatccaatag ccagggcaat agccacaatg acggaaatga tgattgtcat cttgccacgc
    45361 cacaactgca ctagtaaatc aatcaaatca atctgttccg ggtcatggtt ttgcccagaa
    45421 acattattat tttctactct catcattacc ctaactgacg gaaatttcca agtccgcata
    45481 gtttagcgat aatcctcgtg aatgctatag gataagtgat aaaaataatg aattgttgcg
    45541 taagtaacaa aagtcaatca gggcgtaaat agccctgata acaagatgtt agtcgctgcc
    45601 aaagagatcg cgggtgtaga ccttatctgc cacatcccta agctcttctg ccatacggtt
    45661 ggagataatg acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca ggcgagagtt
    45721 gaagaatgag tcttctttca tcactggctc gtagatgatt acttcaacac ctttcgcctt
    45781 gatacgcttc ataatcccct gaatagaaga cgcacggaag ttatctgaac cgctcttcat
    45841 aatcaggcga taaataccca ccacatgcgg tttgcgtgac aaaatggcat cggcaataaa
    45901 atctttacgc gtgcggtttg catcgacaat tgccgagatc aggttattcg gcacagactg
    45961 atagttcgcc agtaactgct tggtatcttt cggcagacaa taaccaccat aaccaaacga
    46021 cggattgttg taatggttgc caatacgcgg atcgagacaa acgccttcga taatctggcg
    46081 agtattcaga cctaaacttt ctgcatagct atccagttca ttaaagtacg ctacgcgcat
    46141 cgccagatag gtattagcga aaagtttaat cgcttctgct tcggttgagt cggtaaacag
    46201 ggtcgggata ttttgcttaa tcgcgccttc ctgtaacaga gcagcaaaac gttctgcgcg
    46261 ttctgaacgc tcaccgatga caatacgtga aggatggaga ttatcgtaaa gggctttacc
    46321 ctcacggaga aattccggag agaaaataat attttcagta cgatatttct tatgcatcgc
    46381 tgcggtaaag ccaacgggta ccgttgattt gatgaccata accgcataag gatttatctc
    46441 tactacgtcc ttaattactg attctacact cgatgtattg aaataattag tgttaggatc
    46501 atagtcggtt ggagtggcga tgatgacata atcagcatcc cggtaggctt catttttatc
    46561 taatgtggca ttaaagtgta ttttatctga ttgcaaaaac tgctgaattt ccttatcaac
    46621 aataggagat atccgatcat tcagcatagc aacgcgtgac ggtaaaatat ctaatgccac
    46681 aacctcatga ttttgtgcga ttagaagccc gtttgacaag cctacatagc cggttccgga
    46741 aatggtgatt ttcatttcat gctctcagaa ttaacttaac tgtgaatcat gatgttttta
    46801 gcatcctgat aagagctaaa agttttaacg ctacgttgtt cgatggtcaa cgcaacgaaa
    46861 aacaattcag attttcttta taagaatagc tgatatttat taagttaata ttaagcagtg
    46921 aaatttagtc tataagataa atggcaaaaa aaagcccggt gcaatatacg ccgggcctca
    46981 attttactgt tggttaaatc agattaatcc agccattcag tatggaacac accttcttta
    47041 tcaatgcgct tataagtatg tgcaccgaaa tagtcacgct gtgcctggat caggttcgca
    47101 ggcagaacag cggcacggta gctgtcataa taggcaaccg cagcggcgaa ggtcggaacc
    47161 gggataccgt tctgtactgc ataagcaacg acatcacgca gcgcctgctg gtaatcatcg
    47221 gcaatttgtt taaagtacgg agccagcagc aggttagcga tctgcggatt ttcggcataa
    47281 gcatcggtga ttttctgcag gaactgcgca cggatgatgc agccagcacg gaaaatcttc
    47341 gcgatttcgc cgtagttcag atcccagttg tactcttcag acgcggcgcg tagctgagag
    47401 aagccctgag cgtaagaaac gattttgccc agatacagcg cacggcgaac tttttcgatg
    47461 aactcagcct tgtcgcctgc tggctgtgct tgcgggccag agagaacttt agatgcggca
    47521 acacgctgat ctttcagaga agagatataa cgtgcaaaca cagactcggt aatcagcgac
    47581 agcggttcgc cgagatccag cgcactctgg ctggtccatt tgcccgtacc tttgttagcc
    47641 gcttcatcca gaatcacatc aaccaggtag ttaccgtctt catctttttt ggtgaagata
    47701 tctttggtga tgtcgatcag gtagctgctc agttcaccgt tattccactc ggtaaaggtc
    47761 tgcgccaatt cttcgttggt gaggttcagg ccacctttta gcagagaata ggcttcagca
    47821 atcagctgca tatcaccgta ttcaataccg ttgtgaacca tcttcacata gtgacctgca
    47881 ccatcggcac caatataggt aacgcatggc tcaccgtctt cagccactgc ggcaattttg
    47941 gtcaggatcg gcgcaaccag ttcataggct tctttctgcc caccaggcat aatggaagga
    48001 cctttcagcg cgccttcttc accaccggag acaccggtac caatgaagtt aaagccttcg
    48061 gcagaaagct cacgattacg acgaatggtg tcctggaaga aggtattacc accatcaatg
    48121 atgatgtcgc ctttatcgag gtatggcttg agggaatcaa tagcagcatc cgtgcctgca
    48181 cctgctttca ccattaacag gatgcgacga ggcgtttcca gagattcaac aaactctttc
    48241 accgtatagt aaggaaccag tttcttgcct ggattttcgg caatcacttc ttccgtcttt
    48301 tcacgggaac ggttgaaaac agagacggta taaccacggc tttcgatgtt aagcgcgagg
    48361 ttgcgtccca tcacagccat accgacgacg ccgatctgtt gctttgacat tgttta
//
LOCUS       DAZGSP010000026        48214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZGSP010000026 DAZGSP010000000
VERSION     DAZGSP010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.026"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..248)
                     /gene="ydjO"
                     /locus_tag="ACHK74_001649"
     CDS             complement(<1..248)
                     /gene="ydjO"
                     /locus_tag="ACHK74_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFP0220490.1"
                     /translation="MFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFISTGQC
                     DCDEIAESNFNRMIAYMSNINYDASKPGAPALSFDTYL"
     gene            complement(537..800)
                     /gene="cedA"
                     /locus_tag="ACHK74_001650"
     CDS             complement(537..800)
                     /gene="cedA"
                     /locus_tag="ACHK74_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFP0220491.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWATQIRQEGEVTE"
     gene            983..3244
                     /gene="katE"
                     /locus_tag="ACHK74_001651"
     CDS             983..3244
                     /gene="katE"
                     /locus_tag="ACHK74_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFP0220492.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3291..4049)
                     /gene="chbG"
                     /locus_tag="ACHK74_001652"
     CDS             complement(3291..4049)
                     /gene="chbG"
                     /locus_tag="ACHK74_001652"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFP0220493.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVASFAAEEGIALRIDRQPLSNTG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNLIRKSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(4062..5414)
                     /gene="celF"
                     /locus_tag="ACHK74_001653"
                     /gene_synonym="chbF"
     CDS             complement(4062..5414)
                     /gene="celF"
                     /locus_tag="ACHK74_001653"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP0220494.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5519..6361)
                     /gene="chbR"
                     /locus_tag="ACHK74_001654"
     CDS             complement(5519..6361)
                     /gene="chbR"
                     /locus_tag="ACHK74_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFP0220495.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6369..6719)
                     /gene="chbA"
                     /locus_tag="ACHK74_001655"
     CDS             complement(6369..6719)
                     /gene="chbA"
                     /locus_tag="ACHK74_001655"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFP0220496.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6770..8128)
                     /gene="chbC"
                     /locus_tag="ACHK74_001656"
     CDS             complement(6770..8128)
                     /gene="chbC"
                     /locus_tag="ACHK74_001656"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFP0220497.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(8213..8533)
                     /gene="chbB"
                     /locus_tag="ACHK74_001657"
     CDS             complement(8213..8533)
                     /gene="chbB"
                     /locus_tag="ACHK74_001657"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFP0220498.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8832..9170)
                     /gene="osmE"
                     /locus_tag="ACHK74_001658"
     CDS             complement(8832..9170)
                     /gene="osmE"
                     /locus_tag="ACHK74_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFP0220499.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9372..10199
                     /gene="nadE"
                     /locus_tag="ACHK74_001659"
     CDS             9372..10199
                     /gene="nadE"
                     /locus_tag="ACHK74_001659"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFP0220500.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10429..11316
                     /gene="cho"
                     /locus_tag="ACHK74_001660"
     CDS             10429..11316
                     /gene="cho"
                     /locus_tag="ACHK74_001660"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFP0220501.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHIRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(11276..11851)
                     /gene="ves"
                     /locus_tag="ACHK74_001661"
     CDS             complement(11276..11851)
                     /gene="ves"
                     /locus_tag="ACHK74_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFP0220502.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(12054..12539)
                     /gene="spy"
                     /locus_tag="ACHK74_001662"
     CDS             complement(12054..12539)
                     /gene="spy"
                     /locus_tag="ACHK74_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFP0220503.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12869..13837)
                     /gene="astE"
                     /locus_tag="ACHK74_001663"
     CDS             complement(12869..13837)
                     /gene="astE"
                     /locus_tag="ACHK74_001663"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFP0220504.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13830..15173)
                     /gene="astB"
                     /locus_tag="ACHK74_001664"
     CDS             complement(13830..15173)
                     /gene="astB"
                     /locus_tag="ACHK74_001664"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFP0220505.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLRGGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(15170..16648)
                     /gene="astD"
                     /locus_tag="ACHK74_001665"
     CDS             complement(15170..16648)
                     /gene="astD"
                     /locus_tag="ACHK74_001665"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFP0220506.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16645..17679)
                     /gene="astA"
                     /locus_tag="ACHK74_001666"
     CDS             complement(16645..17679)
                     /gene="astA"
                     /locus_tag="ACHK74_001666"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFP0220507.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17676..18896)
                     /gene="astC"
                     /locus_tag="ACHK74_001667"
     CDS             complement(17676..18896)
                     /gene="astC"
                     /locus_tag="ACHK74_001667"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFP0220508.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            19342..20148
                     /gene="xthA"
                     /locus_tag="ACHK74_001668"
     CDS             19342..20148
                     /gene="xthA"
                     /locus_tag="ACHK74_001668"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFP0220509.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            20315..21025
                     /gene="ydjX"
                     /locus_tag="ACHK74_001669"
     CDS             20315..21025
                     /gene="ydjX"
                     /locus_tag="ACHK74_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFP0220510.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSSSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            21030..21707
                     /gene="ydjY"
                     /locus_tag="ACHK74_001670"
     CDS             21030..21707
                     /gene="ydjY"
                     /locus_tag="ACHK74_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFP0220511.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAGKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTLVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            21722..22429
                     /gene="ydjZ"
                     /locus_tag="ACHK74_001671"
     CDS             21722..22429
                     /gene="ydjZ"
                     /locus_tag="ACHK74_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFP0220512.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDCMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            22429..22977
                     /gene="ynjA"
                     /locus_tag="ACHK74_001672"
     CDS             22429..22977
                     /gene="ynjA"
                     /locus_tag="ACHK74_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP0220513.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22987..24153
                     /gene="ynjB"
                     /locus_tag="ACHK74_001673"
     CDS             22987..24153
                     /gene="ynjB"
                     /locus_tag="ACHK74_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP0220514.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKVNPSTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            24126..25661
                     /gene="ynjC"
                     /locus_tag="ACHK74_001674"
     CDS             24126..25661
                     /gene="ynjC"
                     /locus_tag="ACHK74_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP0220515.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFAEGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGTGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            25661..26314
                     /gene="ynjD"
                     /locus_tag="ACHK74_001675"
     CDS             25661..26314
                     /gene="ynjD"
                     /locus_tag="ACHK74_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP0220516.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMEQWSENYNKLR"
     gene            26381..27688
                     /gene="ynjE"
                     /locus_tag="ACHK74_001676"
     CDS             26381..27688
                     /gene="ynjE"
                     /locus_tag="ACHK74_001676"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFP0220517.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(27697..28317)
                     /gene="ynjF"
                     /locus_tag="ACHK74_001677"
     CDS             complement(27697..28317)
                     /gene="ynjF"
                     /locus_tag="ACHK74_001677"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP0220518.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            28404..28811
                     /gene="nudG"
                     /locus_tag="ACHK74_001678"
     CDS             28404..28811
                     /gene="nudG"
                     /locus_tag="ACHK74_001678"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFP0220519.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(28777..29049)
                     /gene="ynjH"
                     /locus_tag="ACHK74_001679"
     CDS             complement(28777..29049)
                     /gene="ynjH"
                     /locus_tag="ACHK74_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFP0220520.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            29285..30628
                     /gene="gdhA"
                     /locus_tag="ACHK74_001680"
     CDS             29285..30628
                     /gene="gdhA"
                     /locus_tag="ACHK74_001680"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFP0220521.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(30745..31785)
                     /gene="ynjI"
                     /locus_tag="ACHK74_001681"
     CDS             complement(30745..31785)
                     /gene="ynjI"
                     /locus_tag="ACHK74_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP0220522.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGG
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(31913..33874)
                     /gene="topB"
                     /locus_tag="ACHK74_001682"
     CDS             complement(31913..33874)
                     /gene="topB"
                     /locus_tag="ACHK74_001682"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP0220523.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRALL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVIERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(33879..34922)
                     /gene="selD"
                     /locus_tag="ACHK74_001683"
     CDS             complement(33879..34922)
                     /gene="selD"
                     /locus_tag="ACHK74_001683"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFP0220524.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(35039..35590)
                     /gene="ydjA"
                     /locus_tag="ACHK74_001684"
     CDS             complement(35039..35590)
                     /gene="ydjA"
                     /locus_tag="ACHK74_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFP0220525.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            35751..37607
                     /gene="sppA"
                     /locus_tag="ACHK74_001685"
     CDS             35751..37607
                     /gene="sppA"
                     /locus_tag="ACHK74_001685"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP0220526.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPAVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            37774..38790
                     /gene="ansA"
                     /locus_tag="ACHK74_001686"
     CDS             37774..38790
                     /gene="ansA"
                     /locus_tag="ACHK74_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFP0220527.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            38801..39442
                     /gene="pncA"
                     /locus_tag="ACHK74_001687"
     CDS             38801..39442
                     /gene="pncA"
                     /locus_tag="ACHK74_001687"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFP0220528.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWVRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(39603..39875)
                     /gene="yeaC"
                     /locus_tag="ACHK74_001688"
     CDS             complement(39603..39875)
                     /gene="yeaC"
                     /locus_tag="ACHK74_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFP0220529.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(39917..40330)
                     /gene="msrB"
                     /locus_tag="ACHK74_001689"
     CDS             complement(39917..40330)
                     /gene="msrB"
                     /locus_tag="ACHK74_001689"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFP0220530.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            40672..41667
                     /gene="gapA"
                     /locus_tag="ACHK74_001690"
     CDS             40672..41667
                     /gene="gapA"
                     /locus_tag="ACHK74_001690"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP0220531.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            41751..42635
                     /gene="yeaD"
                     /locus_tag="ACHK74_001691"
     CDS             41751..42635
                     /gene="yeaD"
                     /locus_tag="ACHK74_001691"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFP0220532.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(42686..43540)
                     /gene="yeaE"
                     /locus_tag="ACHK74_001692"
     CDS             complement(42686..43540)
                     /gene="yeaE"
                     /locus_tag="ACHK74_001692"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFP0220533.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(43630..44376)
                     /gene="mipA"
                     /locus_tag="ACHK74_001693"
     CDS             complement(43630..44376)
                     /gene="mipA"
                     /locus_tag="ACHK74_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFP0220534.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            44812..46746
                     /gene="yeaG"
                     /locus_tag="ACHK74_001694"
     CDS             44812..46746
                     /gene="yeaG"
                     /locus_tag="ACHK74_001694"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFP0220535.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            46859..48142
                     /gene="yeaH"
                     /locus_tag="ACHK74_001695"
     CDS             46859..48142
                     /gene="yeaH"
                     /locus_tag="ACHK74_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFP0220536.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT        11933 a        12594 c        11859 g        11828 t
ORIGIN      
        1 tgcagatacg tatcaaaact aagtgctggc gctcctggct ttgaggcatc atagttaatg
       61 ttagacatat aggcaatcat acgattgaag ttcgactctg ctatttcatc gcagtcacat
      121 tgacccgttg atataaagaa agtgagtgcc gccatggtag actcaagcct ttctttaaaa
      181 gagtcgaaag ccaggctttt aatatttaaa tcaccataat tactctgtat taagtttgta
      241 gaaaacatct cccgccccat attgttaaca aaattattat ctcatttaaa tctaagtcac
      301 ttacaatata agtttaagag cgacgccaca ggatgaacta tcaaaaatag ctcatcatga
      361 ttagcaaaac ttaaccattt taaaataaat aaacaattaa agaaaaaaga tcacttattt
      421 atagcaatag ctcgtcaaag gcagcttttt gttacaggtg gtttgaatga atgtagcaac
      481 gaaatgcaga atttcaggtc atgtaactcc cggcaaaacc gggaggtatg taatccttac
      541 tcagtcactt ccccttcctg gcggatctga gttgcccaac gttgggcaga ttcaggcaca
      601 gtaaacgccg gtgagcgcag aaatgactct cccatcagta caaacgcaac atatttgcca
      661 cgcagcatcc agacatcacg aaacgaatcc atctttatcg catgttctgg cggcgcgggt
      721 tccgtgcgtg ggacatagct aataatctgg cggttttgct ggcggagcgg tttcttcatt
      781 actggcttca ctaaacgcat attaaaaatt agaaaaactg tagtttagcc gatttagccc
      841 ctgtacgtcc cgctttgcgt gtatttcata acaccgtttc cagaatagtc tccgaagcgg
      901 gatctggctg gtggtctata gttagagagt cttttgacca aaacagcggc cctttcagta
      961 ataaattaag gagacgagtt caatgtcgca acataacgaa aagaacccac atcagcacca
     1021 gtcaccacta cacgattcca gcgaagcgaa accggggatg gactcactgg cacctgagga
     1081 cggatctcat cgtccagcgg ctgaaccaac accgccaggt gcacaaccta ccgcccctgg
     1141 gagcctgaaa gcccctgata cacgtaacga aaaacttaat tctctggaag acgtacgcaa
     1201 aggcagtgaa aattatgcgc tgaccactaa tcagggcgtg cgcatcgccg atgatcaaaa
     1261 ctcactgcgt gccggtaacc gtggtccaac gctgctggaa gattttattc tgcgcgagaa
     1321 aatcacccac tttgaccatg agcgcatccc ggaacgtatt gttcatgcac gcggatcagc
     1381 agctcacggt tatttccagc catataaaag cttaagcgat atcaccaaag cggatttcct
     1441 ctcagatccg aacaaaatca ccccagtatt tgttaggttc tctaccgttc agggtggtgc
     1501 tggctctgcc gataccgtgc gtgatatccg tggctttgcc accaaattct ataccgaaga
     1561 gggtattttt gaccttgttg gcaataacac gccaatcttc tttatccagg atgcgcataa
     1621 attccccgat tttgttcatg cggtaaaacc agaaccacac tgggcaattc cacaagggca
     1681 aagcgcccac gatactttct gggattatgt ttctctgcaa cctgaaactc tgcacaacgt
     1741 gatgtgggcg atgtcggatc gcggcatccc ccgcagttac cgcaccatgg aaggcttcgg
     1801 tattcacacc ttccgcctga ttaatgccga agggaaggca acgtttgtac gtttccactg
     1861 gaaaccactg gcaggtaaag cctcactcgt ttgggatgaa gcacaaaaac taaccggacg
     1921 tgacccggac ttccaccgcc gcgagttgtg ggaagccatt gaagcaggcg attttccgga
     1981 atacgaactg ggcttccagt tgattcctga agaagatgaa ttcaagttcg acttcgatct
     2041 tctcgatcca accaaactga tcccggaaga actggtgccc gttcagcgtg tcggcaaaat
     2101 ggtgctcaat cgcaacccgg ataacttctt tgctgaaaac gaacaggcgg ctttccatcc
     2161 tgggcatatc gtgccgggac tggacttcac caacgatccg ctgttgcagg gacgtttgtt
     2221 ctcctatacc gatacacaaa tcagtcgtct tggtgggccg aatttccatg agattccgat
     2281 taaccgtccg acctgtcctt accacaactt ccagcgtgat ggcatgcatc gtatggggat
     2341 cgacactaac ccggcgaatt acgaaccgaa ctcgatcaac gataactggc cgcgcgaaac
     2401 accgccggga ccgaaacgcg gcggttttga atcatatcag gaacgcgtgg aaggaaataa
     2461 agttcgtgag cgcagtccat cgtttggcga atattattcc catccgcgtc tgttctggct
     2521 aagtcagacg ccattcgagc agcgtcatat tgtcgatggt ttcagttttg agttaagcaa
     2581 agtcgttcgt ccgtatattc gtgagcgcgt tgttgaccag ctggcgcata ttgatctcac
     2641 tctggcccag gcggtggcga aaaatctcgg tatcgaactg actgacgacc agctgaatat
     2701 caccccacct ccagacgtca acggtctgaa aaaggatcca tccttaagtt tgtacgccat
     2761 tcctgacggt gatgtgaaag gtcgcgtggt agcgatttta cttaatgatg aagtgagatc
     2821 ggcagacctt ctggccattc tcaaggccct gaaggccaaa ggcgttcatg ccaaactgct
     2881 ctactcccga atgggtgaag tgactgcgga tgacggtacg gtgttgccta tagccgctac
     2941 ctttgccggt gcgccttcgc tgacggtcga tgcggtcatt gtcccttgcg gcaatatcgc
     3001 ggatatcgct gacaacggcg atgccaacta ctacctgatg gaagcctaca aacaccttaa
     3061 accgattgcg ctggcgggag acgcacgcaa gtttaaagca actatcaagg tcgctgacca
     3121 gggtgaagaa gggattgtgg aagctgacag cgccgacggt agttttatgg atgaactgtt
     3181 aacgctgatg gcagcacacc gcgtgtggtc acgcattcct aagattgaca aaattccggc
     3241 gtaaatatgg ataaggtcac gtcccctggg gagtgacctt accaccttac ttacacatca
     3301 agataactac ccaaccgata accacgctca gcaattgcat atttcaacga cgctgatgtc
     3361 agcacttcca gctccgttaa ccgtgggaaa cagtaagcac tcttgcgaat caaattatcg
     3421 acaaatgccg gatggcacat gacttccagc gagcgttccc cgcgatggct ggaatcatca
     3481 agcacttgca ggaacagtgc ctcgctaatc tcttcaccat aaaacgcgct actgaatccg
     3541 tgtgaactgc gcagattagc cggtaaatca ccagtgttag atagcggctg acggtcgatg
     3601 cgcaacgcaa tcccctcttc agccgcaaag ctggcaacaa tcgggaaaat ctgcgggaac
     3661 atatgcacat gatgatggct atcaagatgc gtaggtttgc gtccaaatag ctcaatgaaa
     3721 cgcaaatact gactggcaag ctcctgagta atttcttcca gcggtaaagc atcttcttct
     3781 gccaactgcc agatccattt tcccagcaca ccatcgcggg ttaaccccgg catagctgtc
     3841 agtggcttgc ccatagtaag gacaaagtgc atccctatgg ccagactcgg ttcatcacga
     3901 ctcaactgca ccgcatggtc aatagcctgc ccattcacca gcgccgtcgt cgatgtgaca
     3961 atcccattgc gacaggcctc gataatgccg tagttctgac ctttgcttaa gccaaaatca
     4021 tcggcattaa caatcagtaa gcgttccata atcagcctcg gttaatgtgc ttttttaagc
     4081 tctgcgatgc agtcggcaaa atttggcagc catttctcgt gcgccagaat catctcgcgt
     4141 gccagcagct cagcatcgcg atcggaatgc accaacggac taaggtttag cgccagtaac
     4201 acatcgttaa attctccgct aagtgcggcg ttgctggcag caatctcgaa gcctttaatg
     4261 gtgtgaatca gccccatcac tttatcatcg aaatgcgtaa tgcgcggatg tggcgtcgcg
     4321 ccatcgcgcc ccagcttaca ggtcatttct accgcccagt ctgccggaat attatcaata
     4381 tgcccatgat gcgggatatt aacgtaatgt tcagcttgct tgtcgttgta gatagcgttg
     4441 atcacttcgc acgctgcatc agagtaataa gccccaccgc gctgttccag ttctttcggc
     4501 ttaactttca actccggatt tttatacagc tcaaaaagtt gtttctcgac tttctgtact
     4561 acctgtgctc gtgcgccgcc tttgtagtat tcgcccattt caatagccag catctctttc
     4621 tgcttgaagt aatacagcag ataagaacat ggcagcagat ttaacgaacg aattaagccc
     4681 tcactaaatg gcagatcgaa aatatttttt acagaggacg cttttaactg ccctgacgcc
     4741 acaccatcaa gcaattcggc aaagcgcgac ttgccattta ccagcacatc cttaatgaac
     4801 accatatggt tgaggccgaa cagatcgata gataaatcat cgctgtcttt cagcatcaga
     4861 acatcgcgaa taaacatctt catgccgatc ggaatattac acacgccgat aaagcgttta
     4921 aatccggtat gacgataaac ggcttcagtg accattcccg ccgggttagt gaagttaatc
     4981 acccatgcat tcggacaaag ttcttcgaca tcttttacga tgtcaaaaat caccggaatg
     5041 gtacgcagac ctttaaacag accgcccgcg ccgttggttt cctgaccaag ataaccatga
     5101 cttaatggaa tacgttcatc cagttcgcgc gccggtaatt ggcctacgcg cagttgggta
     5161 gtaacgaaat cagcatcttt caatgcttcg cggcgatcca gcgttttata aagcttcatc
     5221 gggacgccag cgttatcaat catccgttgg cagagttcaa aaatgatatc cagtttctct
     5281 ttaccatctt cgacatccac cagccataat tcgctgaccg gcaattcgtg ataacgctta
     5341 ataaatcctt ccagtaactc cggggtatag ctgctcccgc caccaatagt gacgactttt
     5401 aatttctggc tcataatttc tcccttcagt acagaatact gatatctggc atatctgccc
     5461 ccccggacat aaataatcca gcaacagtac agatatgtga attgtcaggt ataacgactt
     5521 actgattaaa ttcagtcaat ttcttacgat agctcttagg cgtaaaggac gttaatttct
     5581 taaacgtttt aataaacaaa ctcgggctac tataaccggc ctcaaacgca atatccgtca
     5641 cggaatagtt ggtcatttcc agttgttttt tggcaaaatt aatacggatc tcattaataa
     5701 tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat tcctgtgatt
     5761 tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct ttatcatgca
     5821 tcttttctac cgtacttttc agccactgcg gtacatcatc aatcacctgt tcttcgcggt
     5881 aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct tccagccctg
     5941 tttcgcggaa attcaatgaa gaaatcactg tttccacata ggtgagaaaa gcgttattgg
     6001 tccggtatac ctgcgaagcg acaaagcaat aaggcaacaa cggcaggtaa tgctgctcaa
     6061 aaaagcgttt actgatccca acgttcaata tacgcgtggc accaaactca taaaaacttt
     6121 gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac acgcgcttac
     6181 cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa tagtcgtgct
     6241 gatgcagccc gctgatactc tcagttttgt tatagataaa cacatggaag tttttgccat
     6301 taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact ggctgcatca
     6361 tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat cagttcacgc
     6421 gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact cactttcatc
     6481 ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc ttcattcaat
     6541 gccatccgtg actgatccat catggctttt gctgcagcaa aatcgccctg cttcgcctgt
     6601 ttcagtgccg catacgccag gctgcgcgct tgtccggagt tgatgataag ccccatcacc
     6661 acttcttcca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag atccatcata
     6721 catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat taaaatttca
     6781 gggcgttagc gatatcttct tcgctctctt ctttatcaat cgcattttgt gctttgttag
     6841 ccaccacaac aaagggcaga taaattaagg ttgcgatgcc aaggttgaag agtgcgacca
     6901 gcaatgcggc gacgctaccg ttggtgttaa agaaggctcc cagaccggtt ggcatggtcc
     6961 acggtgcaat attggtcacc ggaggaatga tgcccatgta gtacgctgcg agggtgattg
     7021 ccgccagaat cggttgtacc agtacaaacg ggataaacat caccgggttc atgataattg
     7081 gcagaccaaa cagaatcggt tcgttaatct ggaagatgcc ggacggcagc gccagttttg
     7141 ccacctgacg ataatcagca cggcgagagg cgataaagat agccaggatc aggcctaaag
     7201 tcgcaccact gcccccaagg aaaataaagg agtccagcat cggcttggcc cagatatgga
     7261 aggtcttacc ggctgccagc gccgcttcga cggaaccata ttgctgatag gtcgcgatat
     7321 tttccagcgc ccacggcgtc ataatgccgt tgtccagtgc ggtcagcgcc agcgcgccat
     7381 gaataccgaa gaaccagagc agtggaacaa agatcacata ggcccagccc accacgctac
     7441 ccaacgatgc cagtggggtt gagatggtat ccataatgat ctgatggaag ttggtgcccc
     7501 aggtattcaa cgcccaggca ataatcccca tcacggaaag aataataaag ccgggaatta
     7561 atgccgagaa ggaacgcgat actgaagcag gtacgctgtc gggcagttta atgacccaat
     7621 tgcggcggac aataaaggta aacatttctg ccaccaccag gccaataata atcccggaga
     7681 tgatattcgc cccacctaac cagtttgcgc caaccgcata ggcctcaccg acactatatg
     7741 gggtgacggt cataaatgct gcaacggata acaaccccgc cgccagcgca tcgactttac
     7801 gctcttctgc cagcgccatg ccaataaaga acggtgccat taaagacatt atccctaatg
     7861 ttccgttata tacgttgccg ccaatacctt tcaggccatt aagtgtttca atggttgagg
     7921 cgtcgaggcg aatacctaag gaataaaaaa acgacccctc cccaaagctt agaaaaacgt
     7981 tgttaattaa tacaaacatg gcccccgcaa gggttaacgg cattaagcga ataaagccat
     8041 ttttgattgc attaacgtgt ggctgctttc ctattttaac tgcaaaaggg aggagtacct
     8101 tttcaagcga tgcaataaca ttactcatag aaaaataccc ttaaaaaccg caatttaaat
     8161 attgcggtat tgatttatga aataactctt tgacgggaaa atttaaaata aattaatttg
     8221 ctgcggcttt tttaatcgct gcaaccgcag ccttaagcac gcctaaacca tcgactttgc
     8281 cataaagcag cgagtcaatt acttcaaccg gtttgttggg taacaaacgc tggatttcgg
     8341 gcaacatata agcaatctgc ggccctaata acacgacatc ggcattctga cctttttcac
     8401 cagccagtgt ttccggaaat gcttcaataa tgaccggaac ttcatatttt tctgcctgtg
     8461 cgcgcatttt tgataccagt aaagaggtag acatgcccgc agaacaaaac agataaatgt
     8521 gtttcttttc cataaaactg ccctcatcga cgattatctg tcagccagac actccgcaag
     8581 ccttaacctg cttccatgct ctgggtaact tgcgaaacca acatgacttt ttttgtggct
     8641 gaaaggagta tacggtatag acctgagcaa tagtatttct ttgaccttat aaattgtctc
     8701 tccttgacct gcggttatga ccctcttcac atttcgggca aataattcgc gaagataatt
     8761 aagaactcag gccaaaaaaa accggcacaa tggccggttt ccgttgttac tcaagctttc
     8821 agacgaattg attacttcgc agcctgtgga tcagtgtcgt attcagcaca ggtctgataa
     8881 ccggagttga tgacatgtcc ggtatcatct aacgcgacaa agtaggtttc tgctttacca
     8941 tcacgttgac ccaggatgta ggtctggcaa gtaccacgag catggatcat gctcacttca
     9001 gacgaaggtt tacccgcaat ttgtgcaacc tgcgcccggc tcatgccttt tttgacgtct
     9061 ttcaccacag gctgtacaaa ctggtcttta gtacgatcat aagccgtaca acccgccagc
     9121 atggttaata ccgccgctgc actcagaatt cctgccatat tcttgttcat attccgtcct
     9181 cttgtttatc agcgtgttag ataagcctgg aatacattgg gcgctttttc aagcccgtga
     9241 acgaaacggc tccgctttca gaggattcct gtatgacgtt ttaaccacca ttcagcccgc
     9301 tgtcgcttgt cgtttcagtg gcaacgggtt agctttaagg aagttttgtc ttttctgtct
     9361 ggaggggttc aatgacattg caacaacaaa taataaaggc gctgggcgca aaaccgcaga
     9421 ttaatgctga agaggaaatt cgtcgtagta tcgattttct gaaaagctac ctgcaaactt
     9481 atccgttcat taaatcactg gtgctcggga tcagcggcgg tcaggactcc acgcttgccg
     9541 gaaagctgtg ccagatggcg attaatgagc tgcgcctgga aaccggcaac gaatcactgc
     9601 aatttattgc cgtacgcctg ccctatggtg ttcaggccga cgaacaagat tgccaggatg
     9661 ccattgcctt tattcaaccg gatcgcgtat taaccgttaa tatcaagggc gcggtattgg
     9721 ccagcgaaca ggcattgcgg gaagcaggca ttgaactgag cgattttgtc cgtggcaatg
     9781 aaaaagcgcg tgagcggatg aaagcacaat atagcattgc gggtatgacc agcggtgtcg
     9841 tggtgggcac cgatcatgca gcagaagcca ttaccggatt cttcactaaa tatggtgacg
     9901 gcggtacgga tattaatccg ctgtatcgtc tcaacaaacg ccagggtaaa cagttgctga
     9961 cggcattagg ttgcccggaa cacctttata agaaagcccc aacggccgat ctggaagatg
    10021 atcgcccttc tctgccagat gaagtggcac tcggcgtgac ctatgacaat atcgacgact
    10081 atctggaagg gaaaaacgta cctgaacagg tcgccagaac aatagagaac tggtatctga
    10141 aaaccgaaca taaacgccgt ccgccaatta ccgttttcga tgatttctgg aaaaagtaat
    10201 aatttgcaca taatggtttg tgcgaaaaag agtaatttgt tcacgccgga tgcggcgtga
    10261 acgccttatt cgacctacaa aactatgcaa attcaatata ttgcaggagt cgaataggcc
    10321 tgacaggcgt agcacttcag acggtgtaac ctttgtcatc gacccgcctc ttttttaatc
    10381 gcttcccgcc tgttacactg gatagataac cagcattcgg agtcaacagt ggtacggcgt
    10441 ttaacttctc cgcggctcga atttgaagct gcggcaattt atgaatatcc cgaacattta
    10501 cgttcattcc ttaatgactt acccacccga ccaggggtgt atctgtttca tggcgaaagt
    10561 gacaccatgc cgctctatat cggcaaaagc gttaatatcc gcagtcgggt gctttctcat
    10621 atacgcaccc cggatgaagc cgccatgcta cggcaatccc ggcgaattag ctggatctgc
    10681 accgcaggcg aaatcggcgc tctgctcctt gaagcgcgat taatcaaaga acaacagccg
    10741 ctgtttaata aacggttgcg ccgcaatcgc cagctctgtg ccctgcaatt aaatgaaaag
    10801 cgcgtcgatg tggtgtatgc caaagaggtg gatttttcac gagcccccaa cctgtttggc
    10861 ctgtttgcca ataggcgcgc agctttgcaa gcattgcaga gcatcgctga tgaacaaaaa
    10921 ctttgttatg gcctgctggg actggaaccg ttaagtcgcg gtcgtgcatg ttttcgttca
    10981 gcgctaaaac gttgcgccgg agcatgctgc ggtaaagaga gccatgagga acatgcgcta
    11041 cgcttgcgcc agtctctgga gcgtttgcgg gtggtgtgtt ggccttggca aggggcggtg
    11101 gcactgaaag aacagcaccc ggaaatgact caatatcata ttattcaaaa ctggctgtgg
    11161 ctgggggcgg ttaattcgct ggaagaagcg acaacgttaa ttcggacacc cgccgggttt
    11221 gatcacgacg gttataaaat tctttgtaag ccgctgcttt ccggtaacta tgaaattact
    11281 gaacttgatc cggcgaatga ccagcgagcc agttgatttc actgaacaac agtttacctt
    11341 gcggttgtaa aagccgcagg gtgtgcctgc catcgaacca gcacgcgccc tggtcggtag
    11401 tgagcaattt atcgcccagt tgccacgccc cgttaatcac aaacaccacc ccgccgcgtg
    11461 agccaaaggt agtaaacgta cgttcggcaa ttctgacttt cgccttacag acatccaggc
    11521 gtgtcatgat gttgaaatcc atcgacatct gccctgccgt cagtttcgct tttaccacct
    11581 ggtccgctgc aaaggcaaaa ggctgtaacg gttttaaggt atggttaaag cggtctgcgc
    11641 tttcaaggag catctcgccg ccttccagca acgtcactat cctttccatg ccgggaaaca
    11701 aagaaaactc cccattcgcc gcgatggatg caatgctggc acgccagtaa aaatcacgtt
    11761 ttgccggtgg gaaagtgcaa atttcgcgcg tttcaccggc agcatttcgc cacagattca
    11821 ccgacatttt acgcatatca aagtattcca tactcgctcc cggcccgttc ttttcattgt
    11881 tatcgttcag gtaaccgtgg atggttatcg gcaactcggg tgatttacgt tagtggtgat
    11941 caggcttttt ttacaactgt ttgagaagag aaaagaaaac cgccgatcct gtccaccgca
    12001 ttactgcaag gtagtggaca agaccggcgg tcttaagttt tttggctgaa agattattca
    12061 gcagttgcag gcattttacc ttttgccgct ggacgttctg tcagacgctt ctcaaaatta
    12121 gcattaaatt gctttttctg ttccggcgtc aggatgttgt aaattttgtt ctgggtttcc
    12181 atgtgcgcca gcatgttagc tttgcgctgt tcttccattt ttgcgatctg cgcttcagct
    12241 tttgctttat cgaaggtatc gctggcaatg atgtcatgca ttgcgcggcg ttcttccagc
    12301 ggcggacgtt tcatctggtc acgctggcct ttcatgattt cgcggatctg ctgtttctgc
    12361 gcgtcggtca ggttcaggtc tttgaacatc atgtcctgat gcggaccgaa cttgcctttg
    12421 tggtgcatca tcggcttcgc gtcagccggt gctgcggtag tggtgtctgc ggcatgggcc
    12481 aggttagccg cgccaagagc cagggtagag gcaacaaaca gtgcagttaa tttacgcata
    12541 ttctatatcc ttcctttcag ttatttatta cggctttctt aagtagcgtg ccgtgttgac
    12601 gagattaact ttactgactt tagcgtcaat taatcagagc aacggtaaaa caatgaaagt
    12661 gtaaaaaaca cttttgcgcc aattatggag aaaaaaagaa aatttgatgg agagtgatga
    12721 gagaatatta caacacgatg attttgcaga gattatgaag aactataccg gatgactggt
    12781 gataaataaa gcaaataacc aggattaatc tgtattaatt tataagaagg caacttaata
    12841 cccgcagaat gatttctgcg ggtaagtatt agcttatttt ttcgagcatt aatcccgcgc
    12901 gtaatcccaa cgctaccaac ggattaggga ataacacata ctctacatca tgggttacgg
    12961 taaaacgttc ctctccgtcc tgcgccagca atgttccttt ctcaaacggc ataaaattca
    13021 gcgtgtcact tgccatatgc atttcgaagg acggcgagtg gcgagtaatt tgcgaaacca
    13081 cccgataacg gagcggcggt gttctcacga taccgacact ctcaccagat agcagcgcag
    13141 caattgcgct ggcagttact gcaaactggc gaagatcgtt ttgcccaaag ggcaacgctt
    13201 tgccaagttc cagcgtacag gccagcgcgc caaaatgtct ggcgctgaaa tgggtaaacg
    13261 taccaccagg ttcctgatgg aacaccagcg cctccagccc cgccgcaccc agccacgtca
    13321 gaaatttctc gtcccaggga atgtcgcgtt gcggtaatac accgaactgc ggatgcaagg
    13381 agccacgaat tgcggtatgt agatcaaggt gccagcgcac agattctttg cccaggtcat
    13441 aaaaatcttc caggcactgt tccagttcgc gcgcccgaca ggtttctccg ctttcagcaa
    13501 atagctgcca acgaccgcca aacattcgat tcatatcgct atggcaataa cgtttccctt
    13561 gcttcagcgc aggaggattc ccgaggatca ccagcaaccg ccaacgtaac gggatctcgc
    13621 cgtgagatat cgcgccaagc aacgcgtcca gcatctccac aggtgccgtc tcattaccgt
    13681 gtattcccgc tgaaatcacc agtgcgcctt gcggtggcgt taatggcgtc agttccagca
    13741 caccatcgcc cagccagcgc cagcgaacgc cgttgatttc tcgctcggtg ataaccggtt
    13801 ttttacccgt taaggtcaga gcaagaaaat tatccattgc cccctccctc gcgctggaac
    13861 ggataaaccg aaccgagatt cagtaattgg ctcaatacat ccagtgcttc ccgccctccg
    13921 cgcagcaatt gcgggtcggc cagatcggca gcagtaaggc gatcgcggta gtaacgatcc
    13981 acccagtcat tgagcgcatt aaacagcgta tcgttcatca tcaccgccgg attcaccgcc
    14041 cggcgttctt cttctgtcaa taccacccgc aaccgcaggc acgccgggcc gccgccattc
    14101 gccatgcttt cacgtaaatc aaagactttt agttcgctaa tcgggttgtc agcggcaagg
    14161 agttcattga gataacccca tactccggcg tgttcccgac actcctgagg cagcaccaac
    14221 atcatggaac catcatcgcg gctcaacagt tggctgttaa acagataggt agacaccgca
    14281 tccgacacgg aaacctgagt tgccggaact tctatcgcca taaaaccgtt cacccgcgca
    14341 cgcaggtttg ccagtaactg tgactggcga gcgaacgcct gttggtggca aaacagcacc
    14401 tggcggttac tcacggcaat cacgtcatta tgaaaaacgc cctggtcgat aacgtccggg
    14461 ttttgctggg cgaaaatcac ctgttgggga ttcacctgat tcagccttgc caccgcctcg
    14521 ctggcttcgc gagtctgtcg cgccggataa cgggaaggcc gggtatcatt gccttcttct
    14581 cgcccgtaga caaaaagttg catacccggc tcaccgtaat gaccgccgag acgattgtgg
    14641 tttgccgccc cctcatcacc gagcaacgct acctgcggca acgccgaatg aacgctaaat
    14701 ttctcttcgt cgttaaaaat cgcttttaaa agtgattcgg tgacgtgcgc ttccagcgag
    14761 cggtgaaatt tattgttcag gttggcaacg gtgagatgca ctttgccatc cagcgtatcg
    14821 gcagatggcg cgatcgttgc cgcattggct acccacattg gcgaagcgga actgacgctg
    14881 gaaagccagt gcggtgcctg gcgtgcaact ttttccagta cctgctcatc gctaccactg
    14941 aatcccaact gacgcagcac cggaataaac ggacgctcgt gcggcgggat cacggcctgg
    15001 gggaatcccg catcggcaag ggctttcatt ttcagtaagc cctgttttgc cgcctgtcgc
    15061 gggttagaca cctgaaaacg gtgacgggta gaggcttcat taccaaacga caggccagcg
    15121 taatgatgcg tcagccctac cagcccgtcg aaattaactt cccaggcgtt catcgcacca
    15181 cctcatcgga aaaatccagc ccggggttaa gcgttgcggg caaagttaac gagtccgact
    15241 ccaggctcgc cataggccat gcgcagtaat ctgcggcata ccaggcgctg gggcgatggt
    15301 taccggatgc accaatgccg ccgaatggcg cggtactggc agcaccagta agcggtttgt
    15361 tccagttaac aatccccgcc cgcgcctcca gcaacagttg atcgaatttt tcccgctcgg
    15421 gggaaaccag accgcaagag agtccgaagc gagtgttatt cgccattaga atcgcttcct
    15481 cgaaagaatc ataacgccag acgcgcaata acggtccgaa cacctcttca tctggtacgc
    15541 cagcaacgcc tgtcatttca atgatccccg gcgtcagcaa cgatgtctct gattgtaata
    15601 agcgcggcgc aagcagggtt cgtccgccca tcgcttccag ttgctgccag gcagtaacca
    15661 cctgctgtgc ggcctgttca gaaatcagcc cgccaataaa cggctgcggt tcgtcatccc
    15721 agttgcccgg cgttaatcgc tggctgacgg caaccagacg agcaagaaac gcatcgccct
    15781 gcgctccgct tttgagcaat aaacggcggg cgcaggtgca gcgttgcccg gctgtgacaa
    15841 acgccgactg aatggtcaga tggacagccg cgtcgatatc cgccacctca tcgataatta
    15901 gcggattatt accgcccatc tcaagggcga gaattttctc cggctgaccg gagagctggc
    15961 gatgcagctg gtagccggta ttggcgctac cggtaaacag caaaccgtcg agatcctcca
    16021 gcgcactcag cgcctgaccc gtttcacgcc cgccctgcac caggttaagt acgcccggcg
    16081 gcaagccagc ctgctgccat aaacgcatta ccgcttcgcc actccacggt gtcagttcgc
    16141 tgggtttaaa gataatggtg ttacctgcca gcaatgccgg aacgatatgt ccgttcggca
    16201 aatgaccagg gaaattatac ggcccaaaca ccgccagcac gccgtgcggg cgatgtcgca
    16261 ggctcgccgc accgtccggc atttcactac gctgctcgcc ggtacgaacg tgatacgcct
    16321 taattgatat cgcgatttta ttgatcatcg ccgtcacttc ggttgccgct tcccagcgcg
    16381 gcttacccgt ttctctggca ataatcgcgg ttaattcgcc tttattcctt tccagcaatc
    16441 cggcaaagcg ttcgacaacg acctgacgtt cagccaatga gagccgcgcc cagcgcggaa
    16501 acgccgcacg ggctgcgcga caagcctgcc cgacctgagc ggcatcggca tcattgcctt
    16561 gccataacac ctcgcccgat accggattac gcttcacacg cgatgcgccc tggcccgtta
    16621 tccagtcacc gttaatccat aaagtcatgc tgttttctcc tctgcgcaca ggcgcaccag
    16681 acgaacgcga tcccctgcgt ggcatttgag ggcatccagt tgtgcggcgg ttaaaatcaa
    16741 acgctcggtt gccggatcgg tacgcgccag caccacgcgg aaatggtgat aattttcatt
    16801 ggcgaccagg caggccggaa aatcgccctg cgcaggctgc ccttctgcca cttccaccag
    16861 ccgactttta cgaatggcgc gcacgcggtc gatgtcacac tcaagcgttg gcccgccgtc
    16921 gaagatgtcg atatagttgc ggtaacgaaa accttctttc tccagcaccg cgcgggcagg
    16981 cgcggtttgc ggatgtacct gaccgatgac gtcctgggct tcctgggata aaaagtgggt
    17041 atagatcgga tgtttcggca tcagttctgc aataaatgcc ttttgcccgg tgccgcagag
    17101 aaaatcggcg cggctaaaat ccatcgaaaa gaagcgttta ccgaggcttt gccagaacgg
    17161 tgaatagccg tgttcgtcaa tcaccccgcg catttcagca accactttgt cattaaactt
    17221 gtcgcgaaaa gccgccataa acataaagcg cgatttcgac agcaaatagc cgttgccctc
    17281 tttgcgccag tccgggtcga gaaacagcgt gcacagctcg ctgctgccgg tgtgatcgtt
    17341 actgagaaac agcgtcggca atgcgttata gacattcagc tcttttgagg cgtgaaccaa
    17401 cgtgccgacg cgatagttgt accagggatc gttcagccca accgccacct caatggcaca
    17461 aatccccgcc acggtgcctg tctcgctatc ttccagcacg aacacatagc cctgctcact
    17521 tttgggcagt tcgccttgcc aggttttgat tgccctttcg atacgcgccg aaagcgtggc
    17581 ttcattggcg ggaagcgacg tcaggccgcc gcccgttttg ctggcaagct gcatcagcgc
    17641 cgagacatct gatcgctcaa cgggacggat gaccatcatg atgaacctcg gctaacaaag
    17701 tgttcgcaag cagctgcaaa gcgatccagt ccggtcgtca cctcttcttc gctgacattg
    17761 agcgcaggcg caaaacgcac cacgttgcca cccgcaatca gtaccatcac gcctgctttc
    17821 gccgcttcct gagagatctg tttcgcttgc ccggcgtaat cggcattcag tacacagcca
    17881 atcagcaaac ctaagccgcg aacttcactg aacaaaccat agcggtgatt aatagtatta
    17941 agacgctcaa caaaccagtc gtgacgctgt ttaacgccat taagcatctc tggtgtgttg
    18001 atgagctcca gcactttgcc tgccaccgcc gaggccagcg gattaccgcc ataggtggtg
    18061 ccatgagtgc caacggtcat cacgcgggcg cactcttcgg ttgccaacaa cgcaccgacc
    18121 gggaaaccgc cgcccagcgc tttggcggta gttaacagat caggcgtcac gccgtagtgc
    18181 atataggcat acagttcccc ggtgcgcccg acgccggttt gtacttcatc aaaaatcaac
    18241 agcgcattgt ggcggttaca caattcacgc agaccttgta aaaacgcgtt gctggctggc
    18301 accacaccgc cttccccctg gatgggttcg acaatcaccg cacaggtaga gtcgtcaatc
    18361 agcgcgctgg cagagttaat atcgttatat gcagcatgac gaatatccgc cggcagtggc
    18421 gcaaaatcct gtgaataggc tggctgccca cccgcactga cagtaaacag cgtgcgacca
    18481 tgaaacgcat ttttgaacgc cacgatgccg ctcttatggc tgccgtagcg gtcgtgagcg
    18541 aatttacgcg ccagttttag cgccgcttcg ttggcttccg caccggagtt acaaaagaag
    18601 acgcgatcgg caaacgtggc gtcgatcaat tttttcgcca gtcgcagtac cggctcgttg
    18661 gtgtaaccgt tgccggtatg ccagaactta ctcgcctgtt cgttcagcgc ttcacgcagt
    18721 tccggatgcg catggcccag cgcgttcacc gcaatgccac ccgcgaagtc gatatactct
    18781 ttcccctgct gatcccacaa gcgcgaacct tcgccacgta ccggtataaa gggtgccgga
    18841 gcgtaaacag gtatcatcca ttcatcaaag ttttcacgcg taattggctg agacatagcg
    18901 acctctacag taaataattc gttatttata tgttaacaat aagtaatgtt tgcgctgtaa
    18961 atgtagattg cagggttcgt gccagccagt gataaaagtg cataaacggc ggaggctaac
    19021 tggaaatcaa ggagttataa ccaaaccata tgcatttaaa gtgcatataa agtgaatacg
    19081 tttgcgatgt gggtgaataa aaagaataaa aaacgcaatg ttatgcagaa gtaaaatata
    19141 attctggaat tgtgatcatt gacgaaattt actggaaatt actgcgccat tctgacgcag
    19201 cgcgcaccaa aagcgggcat tttttgcgcc atcgttgaca tcattaacaa ccatcgatca
    19261 aatcacttaa caacaggcgg taagcaacgc gaaattctgc taccatccac gcactcttta
    19321 tctgaataaa tggcagcgac tatgaaattt gtctctttta atatcaacgg cctgcgcgcc
    19381 agacctcacc agcttgaagc catcgtcgaa aagcaccaac cggatgtgat tggcctgcag
    19441 gagacaaaag ttcatgacga tatgtttccg ctcgaagagg tggcgaagct cggctacaac
    19501 gtgttttatc acgggcagaa aggccattat ggcgtggcgc tgctgaccaa agagacgccg
    19561 attgccgtgc gtcgcggctt tcccggtgac gacgaagagg cgcagcggcg gattattatg
    19621 gcggaaatcc cctcactgct gggtaatgtc accgtgatca acggttactt cccgcagggt
    19681 gaaagccgcg accatccgat aaaattcccg gcaaaagcgc agttttatca gaatctgcaa
    19741 aactacctgg aaaccgaact caaacgtgat aatccggtac tgattatggg cgatatgaat
    19801 atcagcccta cagatctgga tatcggcatt ggcgaagaaa accgtaagcg ctggctgcgt
    19861 accggtaaat gctctttcct gccggaagag cgcgaatgga tggacaggct gatgagctgg
    19921 gggttggtcg ataccttccg ccatgctaat ccgcaaacag cagatcgttt ctcatggttt
    19981 gattaccgct caaaaggttt tgacgataac cgtggtctgc gcatcgacct gctgctcgcc
    20041 agccaaccgc tggcagaatg ttgcgtagaa accggcatcg actatgaaat tcgcagcatg
    20101 gaaaaaccgt ccgatcacgc ccccgtctgg gcgaccttcc gccgctaatt tagcagctct
    20161 cctggctcaa actgggtcag gagaattaac cttgagaaaa atcaacaaac tgtcagtaat
    20221 gatttgttgc ctgccgtcct ttgttatacc gtcactgcgt ttttagttgt ctgaccactt
    20281 ctctattatc aagtttgata taggaaactc cacgatgaac gctgagcgta actttctgtt
    20341 tgcctgtctt atttttgcgc tggtcattta cgctatccac gctttcggtt tattcgatct
    20401 gctcaccgat ttaccccact tacagacact cattcgccag agcggattgt tcggttatag
    20461 cctctatatt ctgttattca tcattgccac cctctttctg ttaccaggaa gcatactggt
    20521 gatcgctgga ggaatagttt ttggcccgct cttagggaca ctactctcat taattgccgc
    20581 aacgctggcc tcctcgagct cattcctgct ggcgcgctgg ctgggacgtg atttactgct
    20641 gaaatacgtg ggccatagcc ataccttcca ggccattgaa aaaggcattg cgcgtaacgg
    20701 tattgatttt cttattctga cccgcttaat cccgttgttt ccttacaata ttcaaaatta
    20761 cgcttacgga ttaaccacca tcgccttctg gccttatacc cttatttcgg cactcacgac
    20821 cctgcccggt attgttattt ataccgtgat ggcaagcgat ctcgccaatg aaggcattac
    20881 gctgcgcttt attttacaac tctgtctggc gggcctggcg ctgtttattc tcgtccagct
    20941 cgcaaaactc tacgcccgac acaaacatgt ggatctgtct gcttcgcgcc gcagcccact
    21001 tactcaccca aaaaatgaag gatagaacga tgtcgcaaca ttattcagtg tcatggaaaa
    21061 aaggactggc tgcactctgc ttactggctg ttgccgggct tagcggctgc gatcaaaaag
    21121 agaatgcggc gggaaaagtg gaatacgacg gactttcgaa cagccaaccg ctgcgcgtcg
    21181 atgccaataa ccatacggta accatgctgg tgcaaattaa tggtcgtttc ctcaccgacg
    21241 acactcgtca cggtattgtg tttaaagatg gctccaacgg acataaatcg ctgtttatgg
    21301 cttatgcgac cccgaaagca ttttatgaag ccctgaaaga ggcaggtggt acgccgggcg
    21361 aaaatatgac gatggataat aaagaaacga ctcttgtcac aggcagcaaa ctggatattt
    21421 cggtcaactg gcaaggggcg gcaaaagcgt attccttcga tgaagtgatt gttgacagta
    21481 atggcaagaa actggacatg cgctttggcg gtaatttaac ggcagcagaa gagaagaaaa
    21541 cgggttgcct ggtgtgtctg gatagctgcc cggtcggcat cgtcagcaat gcaacataca
    21601 cttatggtgc ggtggaaaaa cgcggcgaag tgaaattcaa aggcaatgcc tcagttctcc
    21661 cggcggataa cacgctggca acggttacct ttaaaatcac cgaataaagc caggataaag
    21721 gatgatgaag atacaatcgc gtaaaatctg gtactaccgt ataaccctca tcatcctgtt
    21781 gttcgctatg ctgctcgcat gggcgctgct tcccggcgtc catgagttta tcaatcgcag
    21841 cgttgcggcg tttgccgccg tggaccaaca gggtatagaa cgctttattc agtcttacgg
    21901 cgcactggcg gcagttgtct cgttcttgtt gatgatttta caggccattg ctgcaccgct
    21961 acctgcgttt ttgatcacct ttgccaatgc gtcgctgttt ggcgcgttct ggggcggctt
    22021 gctgtcgtgg accagttcga tggccggcgc ggcgctgtgc ttttttatcg ccagagtgat
    22081 gggccgcgaa gtggtggaaa aattaaccgg caaaaccgtg cttgactgta tggacggctt
    22141 tttcactcgc tacggcaaac acaccatcct ggtctgtcgg ttattgcctt ttgttccttt
    22201 cgatccaatc agctatgctg ccggtctgac ttcaatacgt tttcgctcgt tttttatcgc
    22261 caccgggctt ggtcaattac cggcgactat tgtttattcc tgggcgggca gcatgttaac
    22321 aggcggtact ttctggtttg tcaccggact gtttattctg tttgccctga ccgtggtgat
    22381 ttttatggcg aagaaaatat ggcttgagcg ccagaagagg aatgactgat gggtttaccg
    22441 ccgcttagca aaattccttt cattttacgt ccgcaggcgt ggctgcatcg tcgccattac
    22501 ggcgaggtgc taagcccgat tcgctggtgg gggcggatcc cgtttatctt ttatttggtg
    22561 tcaatgtttg ttgggtggct ggagcgcaaa cgctcaccgc tcgatccggt agtgcgatcg
    22621 cttgtcagcg cgcgcattgc gcaaatgtgc ctgtgtgagt tttgcgtgga tatcaccagt
    22681 atgaaagtcg ccgagcgtac cggcagcacc gataaactgc tggcagtggc tgactggcgg
    22741 caaagcccgc tctttagcga tgaagaacgg ctggcgctgg agtacgccga agccgcaagc
    22801 gtcacaccgc caacagtcga tgatgccctg cgtacccgac tggctgcgca ttttgacgct
    22861 caggcgctca ccgaactgac ggcattgatc ggcctgcaaa acctgtcggc tcgttttaat
    22921 tctgccatgg acattcccgc tcaggggctg tgccgtattc ctgaaaaacg ttcttaagga
    22981 gagatgatgc gccattgtgg gtggttgctg ggattgttat cgctgttttc tttggcaaca
    23041 catgccagtg actggcagga aattaaaaat gaggccaaag ggcaaaccgt ctggtttaac
    23101 gcctggggcg gcgataccgc aattaaccgc tatctcgact gggttagcgg cgagatgaaa
    23161 acccattacg ctataaacct gaagattgtt cgtctcgcgg atgccgcaga cgcggtgaag
    23221 cgcattcaga ccgaagctgc tgccggacgt aaaacgggcg gctcggtgga tctgctctgg
    23281 gtgaacggcg aaaacttccg caccttaaaa gaggccaatt tattacaaac gggctgggcg
    23341 gagactctgc ccaactggcg ctatgtcgac acacagctgc cggtgcggga agatttttca
    23401 gtgccgacac aaggtgcgga atcgccctgg ggcggcgcac aactgacgtt tatcgcccgc
    23461 cgcgatgtta cgccacagcc accacaaacg ccgcaagcct tactggagtt tgctaaagtc
    23521 aatcccagca cggttaccta tccgcgccca ccggacttta ccggcacggc gtttcttgaa
    23581 cagttgctga ttatgctgac gcccgatccc gcagcattaa aagaagcgcc ggacgatgcg
    23641 actttcgccc gtgtcactgc tcccttgtgg caatatcttg atgcgctaca tccgtatttg
    23701 tggcgcgaag gaaaggattt cccgccttcg cccgcgcgga tggatgctct gctgaaagcc
    23761 ggcacattgc gcctgtcgct gacctttaac cccgcgcatg cgcagcaaaa aatcgccagc
    23821 ggcgatttgc cggcaagcag ttacagtttt ggctttcgcg aggggatgat tggcaacgtg
    23881 catttcgtca ccattccagc caacgcgaat gccagtgctg cggcgaaggt agttgccaat
    23941 ttcctgctct cacccgatgc gcaactgcgt aaagcagatc ccgttgtctg gggcgatcct
    24001 tctgttctcg atccgcaaaa actgcctgat gggcagcgcg aaacattgca atcaagaatg
    24061 ccgcaagatc tgccgccggt actggctgaa ccgcacgcag gttgggtaaa tgcgctggaa
    24121 caagaatggc tacaccgtta cggtacgcat taatcttttt gctgtgggcg atggtggcgg
    24181 tgatttatgc accactgatc ccggcagctc tcacgctgat atcgcctgct ttgtcgttga
    24241 cacactggca ggcgttattt gccgatccac agttaccgca ggcattactg gcaacgctgg
    24301 tgtcgacaac catcgcggcg gtcggggcgt tgttgattgc cctgctggtg attgtggcgc
    24361 tgtggccttg gccgaaatgg cagcgtatgt gtgcccgtct gccgtggctg ctcgccattc
    24421 cccatgtggc ttttgccacc agcgcccttc tgctctttgc tgagggaggg ctgctttatg
    24481 actatttccc gtatttcact ccgctaatgg acaaattggg tattgggctg ggcctcaccc
    24541 ttgcagtgaa agaaagcgca tttctgctgt ggatcttagc ggcagtattg agcgaaaaac
    24601 ggctgttgca gcaggtcatt gtgctggatt cgctgggcta cagccgctgg caatgcctga
    24661 actggctgct cttgccctcc gtcgcacctg cactggcaat ggcgatgttg gcgattgttg
    24721 cctggtcgct ttcggtcgtg gatgtggcga ttattcttgg gccaggtaat ccaccgacgc
    24781 tggcagtaat tagctggcag tggttaactc agggcgacgc ggatcaacaa acgaaaggcg
    24841 cactcgccag tctgctgctg atgctgttac tcgccgccta cgttttgctg agctatctgc
    24901 tatggcgcag ctggcggcgc actattcccc gcgtagatgg cattcgcaag cctgccacac
    24961 ctttattacc aggcactacg ctggcgagtt ttttaccctt aaccggtgtg ctgtgtgtgg
    25021 ttctgctggc gatcctcgcg gatcagtcga cgatcaatag tgaagcactc atcaacagcc
    25081 tgacaatggg gctggtggcg acattcatcg ctttgctcct gttactgctg tggctggaat
    25141 gggggccaca gcgtcgccag ttgtggctct ggttgcccat tctattgccc gctctgccac
    25201 tggtggcggg ccagtacacg ctggcgctat ggctgaacct ggatggaagc tggacggcgg
    25261 tggtctgggg gcatctgctg tgggtaatgc cgtggatgct gtttatcctg caacccgcct
    25321 ggcagcgcat tgattcacgg ttaattttga ttgcgcaaac actgagctgg tcgcgggcca
    25381 aaatcttctt ttacgtgaaa tgtccgctta tgctacgccc tacgctgatt gccttcgcgg
    25441 tgggattttc agtcagtatt gcgcagtata tgccgacgct gtggctgggc acggggcgtt
    25501 ttccgacgct caccactgag gcggtggcat taagcagcgg cggcagcaat ggtattttcg
    25561 ccgcccaggc tttatggcaa ctgctattac cgcttattat ttttgccctg accgcgttag
    25621 tcgcaaaatg ggtaggttat gtcagacaag gactccgcta atgctctgcg tgaaaaatgt
    25681 ttcgctacgt ttaccagaaa gccgcttgct gacaaacgtt aactttacgg tggataaagg
    25741 tgacattgtc acgttaatgg ggccgtctgg ctgtggaaaa tccactctgt tttcatggat
    25801 gattggtgca ctggctggac agttttcttg tacaggtgag ctatggctca atgagcaacg
    25861 gattgacatg ctacccaccg cacagcgtca gattggcatt cttttccagg atgcactgtt
    25921 atttgaccag ttcagcgtcg ggcaaaattt actgctggcg ctaccgtcga cgctcaaggg
    25981 gactgccagg cgcaatgccg tgaaagacgc gcttgatcga gcgggactgg ctgaaactta
    26041 tcaccaggac cctgcgacat tatcaggcgg tcagcgagcg cgggtggccc tgctgcgcgc
    26101 ccttctcgct cagccaaaag cgttactcct ggatgagcca ttcagccgtc ttgatgtggc
    26161 tctgcgcgat aattttcgcc agtgggtgtt cagcgaagtt cgcgaactgg cgatccccgt
    26221 cgttcaggta acgcacgatc tccaggatgt tcctgctgat agttctgttc tggatatgga
    26281 gcagtggtca gaaaattaca acaaactgcg ataacgcaaa gtttttctca atgcgtcagt
    26341 tcagaatggc gcactcaaaa ctacaatgtc gggattttcg atgaaacgtg tttctcaaat
    26401 gaccgcgctg gcaatggctt tagggctggc ttgcgcttct tcgtgggccg ctgaactggc
    26461 gaagcctctt acacttgacc agcttcaaca acaaaatggc aaagcgatag atactcgccc
    26521 cagcgcgttt tataacggct ggccacaaac cttaaatggc ccttctggtc atgaacctgc
    26581 cgccttaaac ctctctgcca gctggctcga caaaatgagc accgaacagc tcaacgcgtg
    26641 gatcaagcaa cataacctga aagccgatgc cccggtggcg ctgtacggta atgacaaaga
    26701 tgtcgacgcc gtcaaaacgc gactgcaaaa agcaggtttt acgcatatct ccattctgag
    26761 tgacgcgcta agcgaacctt cccgtctaca aaaactgccg cactttgagc agctggttta
    26821 tccgcaatgg ctgcacgacc tgcaacaagg taaagaggtt acggcgaaac ctgccggtga
    26881 ctggaaagtc attgaagcgg cctggggcgc tcctaagctt taccttatca gccatattcc
    26941 cggtgctgac tacatcgata ccaacgaagt ggaaagcgaa ccgctgtgga acaaagtttc
    27001 tgatgaacaa ctaaaagcga tgctggcaaa acacggcatt cgccatgaca ccacggtcat
    27061 tctgtacggg cgtgacgtat acgctggagc gcgtgtggcg caaattatgc tctacgcagg
    27121 cgtgaaagat gtgcgtctgc tggatggcgg ctggcaaacc tggtccgacg cgggactgcc
    27181 tgttgagcga ggaacgccac cgaaagtgaa agcggaaccg gatttcggcg tgaagatccc
    27241 ggcacaaccg cagttgatgc tcgatatgga acaggcccgt ggactgctgc atcgccagga
    27301 tgcatcgctg gtgagcattc gttcgtggcc agaatttatc ggtacgacca gcggttacag
    27361 ctatattaaa ccaaaaggtg aaatagccgg agcacgttgg ggacacgctg gtagcgactc
    27421 gacgcatatg gaagattttc ataacccgga tggcaccatg cgcagcgccg atgatattac
    27481 cgctatgtgg aaagcgtgga atatcaaacc agagcagcaa gtttcattct actgcggcac
    27541 cggctggcgc gcgtccgaaa cctttatgta cgcacgcgcc atgggttgga agaatgtttc
    27601 cgtgtatgac ggcggctggt acgaatggag cagcgatcca aaaaatccgg tagcaaccgg
    27661 tgaacgcggc ccggacagta gcaaataaca ttgcaattac tgacgctgga gcgacttcag
    27721 cgtcagatac ccactccaca cccgcgtaaa ggtcgtcatc cagcacaacg caccgaagat
    27781 ccacgcaaac cacgcaaacc atgcagggaa taaacatccc agcacaaaca gtaagatcgt
    27841 ttctgtgcct tcggttaacc cgcccagata ataaaacgat ttatgcgcat aaccgggatt
    27901 atcaatctga tgtttcgccg ccagcgcagc aaaagcgaga aaactgctgc ctgtgccgat
    27961 aaacgcaaac agcagccagc cgcctgccag ggcgttttgc tgcggtgcgg cgaggataaa
    28021 cccaaaaggt accagcgcgt aaaaaagaaa atcgagagaa atatcaagaa agccgcccgc
    28081 gtcggtaagc cctctgcgcc gcgccagcgc accgtccaga ccatcaagca gcctgttcaa
    28141 caaaatgacg acaagcgccg ccagatacca gcccagcgcc agaaaaggca gtgccagcac
    28201 gccgatggca aatcccacta atgttaaacc atctggcgta atgcccggtt tatcaagaac
    28261 ccgcacgcac tgatgcaaca acggtttaat ccggggatga agatggcggt ctagcacagg
    28321 cactccttaa atataaagcc tttctgattg agcaacagtg cggatattat ggcatttttc
    28381 gcttatctgc ccgtgtgtaa tttatgaaaa tgattgaagt tgttgccgcc atcattgaac
    28441 gtgatggcaa aattttactc gcgcaacgcc ccgcccagag cgatcaggcg ggattatggg
    28501 agtttgccgg tggtaaagtc gagccggatg aaagccagcg gcaggcgctg gtgcgtgagt
    28561 tacgcgaaga actgggcatc gaagcaactg tgggtgagta tgttaccagc catcagcgag
    28621 aagtttcggg ccggattatc catcttcatg cctggcacgt acccgacttc cacgggacgt
    28681 tacaggcaca tgaacatcag gcgctggtct ggtgctcacc tgaagaggcg ctgcaatatc
    28741 cgctggcccc tgctgacatt ccattattag aggcgtttat ggctttacgc gccgccagac
    28801 cagcggatta gtgctaaggg ttttgtcatc ccgctggcat tgcagcagta ttccctcggc
    28861 tttaattacc gcgccttcag aataattttt atcctgataa acgcagcact gagtacaggg
    28921 ctgcgctgac tgcccgcctg aactgaacac ttctggcggt acgtttacct ccacgtccgg
    28981 acgataatgc gggttagcca gtgcaattaa tggaaatgct aatactacgg cgaacaatgc
    29041 tcgactcaca gggaagtcct taacgttatt gtctctgcta ctgataacgg tagccgggtg
    29101 gcaaaacttt agcgtctgag ttatcgcatt tggttatgag attactctcg ttattaattt
    29161 gctttcctgg gtcatttttt tcttgcttac cgtcacattc ttgatggtat agtcgaaaac
    29221 tgcaaaagca catcacataa acaacataag cacaatcgta ttaatatata agggttttat
    29281 atctatggat cagacatatt ctctggagtc attcctcaac catgtccaaa agcgcgaccc
    29341 gaatcaaacc gagttcgcgc aagccgttcg tgaagtaatg accacactct ggccttttct
    29401 tgaacaaaat ccaaaatatc gccagatgtc attactggag cgtctggttg aaccggagcg
    29461 cgtgatccag tttcgcgtgg tatgggttga tgatcgcaat caggtacagg tcaaccgtgc
    29521 atggcgtgtg cagttcagct ctgccatcgg cccgtacaaa ggcggtatgc gcttccatcc
    29581 gtcagttaac ctctccattc ttaaattcct cggcttcgaa caaaccttca aaaatgccct
    29641 gactaccctg ccgatgggcg gtggtaaagg cggcagcgat ttcgatccga aaggaaaaag
    29701 cgaaggtgaa gtgatgcgtt tttgccaggc gctgatgact gaactgtatc gccacctggg
    29761 cgcggatacc gacgttccgg caggtgatat cggggttggt ggtcgtgaag tcggctttat
    29821 ggcggggatg atgaaaaagc tctccaacaa taccgcctgc gtcttcaccg gtaagggcct
    29881 ttcatttggc ggcagtctta ttcgcccgga agctaccggc tacggtctgg tttatttcac
    29941 agaagcaatg ctaaaacgcc acggtatggg ttttgaaggg atgcgcgttt ccgtttctgg
    30001 ctccggcaac gtcgcccagt acgctatcga aaaagcgatg gaatttggtg ctcgtgtgat
    30061 cactgcgtca gactccagcg gcactgtagt tgatgaaagc ggattcacga aagagaaact
    30121 ggcacgtctt atcgaaatca aagccagccg cgatggtcga gtggcagatt atgccaaaga
    30181 atttggtctg gtctatctcg aaggccaaca gccgtggtct gtaccggttg atatcgccct
    30241 gccttgcgcc acccagaacg aactggatgt tgacgccgcg catcagctta tcgccaatgg
    30301 cgttaaagcc gtcgccgaag gggcaaatat gccgaccacc atcgaagcga ctgaactgtt
    30361 ccagcaggca ggcgtactgt ttgcaccggg taaagcggct aatgctggcg gtgtagcaac
    30421 gtcaggcctt gaaatggcgc aaaacgctgc gcgcctgggc tggaaagccg agaaagttga
    30481 cgcacgtttg catcacatca tgctggatat ccaccatgcc tgtgttgagc atggtggtga
    30541 aggtgagcaa accaactacg tgcagggcgc gaacattgcc ggttttgtga aggttgccga
    30601 tgcgatgctg gcgcagggtg tgatttaagt tgtaaatgcc tgatggcgct acgcttatca
    30661 ggcctacaaa tgggcacaat tcattgcagt tacgctctaa tgtaggccgg gcaagcgcag
    30721 cgcccccggc aaaatttcag gcgtttatga gtatttaacg gatgatgctc cccagggaac
    30781 atttcttatg ggccaacggc atttcttact gtagtgctcc caaaactgct tgtcgtaacg
    30841 ataacacgct tcaagttcag catccgttaa ctttctgcga tagcagcaga tatgccagta
    30901 aagaaatccc attagactat ttttttgata atcttcttcg ctttcgaaca actcgtgcgc
    30961 ctttcgagaa gcaagcatta tataatgcca ggccagttct tcttcaattg tcccgttttg
    31021 aaaagctgtg cttgatatcg agatcatcca tgataattcc gccgcccata ttagcttcgc
    31081 cgaggattta ccggagcgat tattagcgca atcagagata tagtctgagg gaaaaacagc
    31141 aaatttattc aacaaggcga taacctgctc tggggcttcc tccatgtttg ctttaaaggt
    31201 attggctcca tggtcgccag aaagaaaatg ctccattaag gcacaataac tttcgctatc
    31261 ttcgataccc cattgatcct ctaaagactc gcgtctttta cttatgatat cgatcgagtc
    31321 aaagggaagc acatgatatt ggaaggtatc tttgccaggt tcaggctttc gcggccagaa
    31381 ctccagcgtt tcagaccatt gcttatgata gaaccgataa ggtgcgatca attgtagcgc
    31441 ctgtaacttc tcgatactga gcggctcaat acctttagcc tgataataat gcagttgttc
    31501 tttttttgct ttaaaaccgg cccgaacaat aagccccatc ataattaata gataaagaaa
    31561 agagcatccc gcagtaatca ggcctctttc attcaaaccg ttggatgtta tcgctgcgaa
    31621 tacaaacatt acagcgacaa cacatgttaa ataccccccc cacttacaaa gcagcatggc
    31681 cttattttct ttaatcatcc gttcaaaatt actattaaat atttcccagc cattaaaaga
    31741 atacttctcg ctcccaggat ggttttgtaa taaaactttt ttcatcacat tccctgttat
    31801 tacatacttt agaggcgata attttatcca tgcaaaaaaa atatccaacg aattttcttg
    31861 atccgctgga tatttcagaa tatgactcga atagcacgaa agattcgctc gcttacgcta
    31921 tcgccccgct tccgacttca tctgctggcg gacttttttt cgcactacgt ttacgcggtg
    31981 cagccttttt cttatcagca ctgccaccac tgcccggagc cacaatgccg cgaaactgcc
    32041 gcaccggcgt acgtttggct tgatcaataa gctgatatag cgtccccacc agcggctgca
    32101 taaagtcctg atagcgacac tgcttttcgc tgatttgcgt cagcaccgat tcccagtgcg
    32161 cggtcatgtc cggtcgcgtc gccatttctg gcagcgaatg gaataacgct tttccggcgt
    32221 cggtagagtg gatatagcgc ccttttttgg tcagaaaacc acgcttgaac agcagttcaa
    32281 taatcccggc acgggttgct tctgtcccca gaccatcggt cgcacgaagg atctttttca
    32341 gatctttgtc ctgcacaaag cgcgcaatcc cggtcatcgc cgaaagcagt gttgcatcgg
    32401 taaaatggcg cggcggctgg gtttgccgct ctatcacttc acctttttca cacagcaact
    32461 catcgccttt cgccaccaca ggcaatggcg tgccgtcgtt ttcttcatcg cgctctttgc
    32521 tgcctaacag cgcgcgccaa cctgcttcag caagaaaacg cgctttagcg acaaatttgc
    32581 ctttggcaat gtccagttcg ataacacact tgcggaacac tgcatccggg cagaactgca
    32641 tcagatactg acgggcaatc aggttataga ccttcgcttc gttctccgtc aggttgatcg
    32701 cagaactcct tgcggtcgga atgatggcgt ggtgcgcatc gacctttttg tcatcccaac
    32761 agcggttgcg tatatctgga tctaccactg gctgcggcaa cagatctggt gcatgaacgc
    32821 tgatggcatt catcaccgcg tggcgtccgg caaaatgttc ttctggcaaa tagcgacaat
    32881 cggaacgcgg ataagtgatt aacttgtgtg tttcgtacag cttctggcag atatcaagca
    32941 cgttctgcgc gctcagacca aagcgttttg ccgcttcaat ctgcaacgcc gaaagcgaaa
    33001 acggcagcgg cgcggattct gattcccgtt tatcgttata gctggtgaca atagccggtt
    33061 gaccactaat gcggttaacc acatgctccg ccagtggacg atgtaacaag cgcccttctt
    33121 catcctggta cggttcacac gcttcgctcg gttgccagat agcggtaaac cgctcatcgg
    33181 caggtgtcac gatatgcgct ttgacttcaa agaagtcttt cgccacgaag ttttcaatct
    33241 cttcatcgcg gcgcaccacc agcccgagca cgggtgtctg cacgcgtccc acggaaagta
    33301 cgccctgata accggcattg cgaccgagaa tggtatacgc acgggtcata ttgatgccgt
    33361 acagccagtc ggcacgcgct cgcgccagcg cagaaacgca caatggcaca aactcactgt
    33421 tggaacgaag acggtcgatc gcccgctcaa ccgcctgcgg gttcaggtcg tttatcaagc
    33481 aacgctgcac ctgctggcgc ttttccggtg ccagttgcag atagtccagc acttcatcca
    33541 ccagcaattg cccttcacga tccgggtccc cggcgtgaac gatttcgctg gcttcatgca
    33601 ggaagcgttt gatgacgtta agttgtttgg tcacggaggg tcggggctgt aattgccact
    33661 tttccgggac aatcggcaaa tccgcaagat tccagcgcgc atagcggctg tcgtaggcgt
    33721 ctggctgcgc ctgctcaagc aggtgaccga tacaccaggt caccacctga ccattaccgc
    33781 actcgataaa gccatcgcct ttccggtgcg gtttgggcag gacatcagca atggcgcgcg
    33841 ccagactcgg tttttcggca ataaacaacc gcattgaatt aacggatctc aaccatggca
    33901 cgaccgccgc gcgctggcac cagttcgcca atcgccgtca gttcaatgcc aaactcggcg
    33961 gctgtagttt taacctcatt ttctgcttcc ggcatgaccg ccagcagcaa accgccagaa
    34021 gtttgcggat cgcacagcag atcgcgcact tcacgcggca tttcacccat cagatgaccg
    34081 tagctggcaa agttacgttc agtgccgcca ggtactgcgc ccaacttaat gtactcttca
    34141 acaccgggga gtttcgggat cgcttcatag tcgacgcgtg cctgcacacc agccccctga
    34201 cacatttcgc tcaagtggcc cagcagacca aagcccgtaa cgtcggtcat cgcttttacg
    34261 ccttcgatgt tggcaaagga cgcgcctgcg atgttcatcc ggcacatcac ttccgtcgcc
    34321 agtccctgat gttctggttt caacagtgat tttttctcag ccgtggtaag aacgccgatc
    34381 cccagcggtt tcgtcaggaa cagtttgcat ccggcttgtg cggtactgtt tttcttcacc
    34441 cgctcggtcg gtacgatccc cgttaccgcc agaccaaaaa tcggctccgg cgcatcgatg
    34501 gagtgaccgc cagccagcgc aatacccgcc tgacggcatg catagcgtcc accttcggtt
    34561 acttcgcggg caatttccgg ggaaagtttg ttaatcggcc agccgaggat cgcaatcgcc
    34621 ataatcggtt tgccccccat cgcgaagata tcgctgatgg cgttagtcgc cgcaatgcgg
    34681 ccaaaatcga aaggattatc aacgatcggc ataaagaagt cggtggtact gataacgctg
    34741 gtgccattgc ccagatcgta caccgccgca tcgtcgcggg tctcattacc cacaagcaaa
    34801 ttcggatcaa caaacttcgc ctgctcactg tgcaggatgg tttccaacac ttttggggaa
    34861 attttacagc cgcaaccagc tccgtggctg tattgggtca aacgaatcga gttctcgctc
    34921 atggacatct cctgtcaatg caatccgggt atggtaaccc tcattccgtg aagtgataag
    34981 tgagagtgtc tgaattcctg cgcctttgct cacaatccag acagtttcgc gacaattatc
    35041 agaaataagt tacaaacggc gtcgggtccg ggacgttaat cgacgtagat gctttcaact
    35101 gcggcgtgcc gaggtagaga aaaccgacaa ttttatcctg ctcacggcaa ccgaatgctt
    35161 cacgcactac cggactttca gttaatgcgc cactgcgcca gatgccgcca aatccttggg
    35221 caacggctgc catttgcatc gccatgaccg cgcatccggc agacatttcc tgttcccatc
    35281 gcgggacttt atgattttct tcgcatttcg ccaccaccgt gatgatgagc ggtgcgcgga
    35341 acggcgcatt acgggctttg tcgatagctt tgtcatcact accggcagca atcgccccct
    35401 gttccagtac ggcgctgaaa cgctcgcgcc cttccccttc aatcacaaaa aaatgccacg
    35461 gttgcatgga cttatggtcc ggcgcacgca tacccgcacg caggatgttt tgcagttgtt
    35521 cacccgttgg cgcgggttca gccaggcggg aggcgctacg gcgattgatc aatagttcga
    35581 gtgcatccat ttgattaact cctgtcgtga tatttattca caaaattaac acgagagtgg
    35641 attttgttac agcacagtcc gcaattcctg ctgacaagtg ccggttgggt cattacgata
    35701 accacatcta ttgcgcctgt gacaggtgtg accttaagtt gggagaatac atgcgaaccc
    35761 tttggcgatt tattgccgga ttttttaaat ggacgtggcg tctgctgaat ttcgtccgtg
    35821 aaatggtact taacctgttc tttattttcc tcgtactggt tggtgtgggg atctggatgc
    35881 aggtcagtgg tggtgattcg aaagaaacgg ccagtcgtgg cgcactgctg ctggacattt
    35941 ctggtgtgat cgtcgataaa cccgacagtt ctcagcggtt tagtaaatta agccgccagc
    36001 tgcttggtgc cagttccgat cgtctgcagg aaaactcact gtttgatatc gtcaacacca
    36061 ttcgccaggc gaaggacgac cgcaatatca ccggtatcgt gatggatctg aaaaacttcg
    36121 caggcggcga ccagccgtct atgcagtaca tcggcaaagc cctgaaagag tttcgtgaca
    36181 gcgggaaacc ggtttatgcc gttggcgaga actacagcca ggggcaatat tatctcgcca
    36241 gtttcgccaa taaaatttgg ctgtcaccgc aaggcgtggt tgatctgcac ggttttgcta
    36301 ccaacggtct gtactacaaa tcgttgctgg ataagctgaa agtttccacc catgtgttcc
    36361 gcgtgggtac gtataaatct gccgttgaac cgtttattcg tgatgatatg tcaccggcag
    36421 cccgcgaagc tgacagccgc tggattggtg agctgtggca aaactatctg aatactgttg
    36481 ccgctaaccg gcagatccct gctcagcagg tattccctgg cgcgcaaggg ttgcttgagg
    36541 gtttaaccaa aaccggtggc gataccgcga aatatgcact ggaaaacaag ctggtcgatg
    36601 cactggcatc gagtgccgaa atcgaaaaag ctctgaccaa agagttcggc tggagtaaga
    36661 ctgataaaaa ttatcgcgcc atcagttatt acgattacgc attgaaaacg ccggcagata
    36721 ccggtgacag catcggtgtc gtcttcgcta atggcgcaat tatggatggc gaggaaactc
    36781 aggggaatgt tggcggtgat accactgcgg cacaaatccg cgacgctcgc cttgacccga
    36841 aagtgaaagc gattgtcctg cgtgttaata gcccaggcgg cagcgttacc gcgtctgaag
    36901 tgattcgcgc tgaactggca gcagcccggg cagcgggtaa gcctgcggtt gtatcgatgg
    36961 gcggcatggc ggcatctggt ggttactgga tttccacgcc agctaattac attgtggcta
    37021 accccagcac cctgaccggt tctatcggta tcttcggcgt gatcaccacc gtagaaaata
    37081 gtctggattc gattggtgtt catactgatg gtgtctcaac ttcaccgctg gcggatgttt
    37141 ctatcaccag ggcactgccg ccggaagcgc agcagatgat gcaattaagc attgagaatg
    37201 gctataaacg ctttatcacg ctggttgctg atgcgcgtca ttcgacgccg gagcaaattg
    37261 ataaaatcgc ccagggccac gtctggaccg gtcaggatgc aaaagctaac gggctggtcg
    37321 atagtctcgg ggatttcgat gatgcggttg ccaaagcagc agagctggca aaagtgaaac
    37381 agtggcatct ggaatactac gttgatgaac cgaccttctt cgacaaagtg atggacaaca
    37441 tgtctggttc tgtccgggca atgttgccag atgcgttcca ggccatgtta cctgcaccgc
    37501 ttgcctcggt agcctctacc gttaaaagtg aaagcgacaa gctggccgcg tttaacgacc
    37561 cacaaaaccg ttatgcgttt tgcctgacct gcgccaacgt gcgttaagtc ttgtactgag
    37621 tggccgacag atcgtcggcc acattatttt ttacctcgac gaatcctctt cccgctgtct
    37681 cgccccatat ttccttatcc gcatagtatc aggtgcgctc ctcctgcctc acgtatatac
    37741 ttttgctctt tcgatatcat tcatatcaat atcatgcaaa agaaatcaat ttacgtcgcc
    37801 tacacgggcg ggaccatcgg gatgcagcgt tccgagcagg gttatattcc ggtgtcaggt
    37861 catctacaac gccaactggc gctgatgccg gaattccatc gcccggagat gccagatttc
    37921 accattcatg aatatacgcc gttgatggat tcttctgata tgacgccgga agactggcag
    37981 catattgctg aagatattaa agcgcactat gacgactatg atggttttgt cattctacac
    38041 ggtaccgaca cgatggcgta taccgcctct gcgctgtcgt tcatgctcga gaatctcggt
    38101 aaaccggtca ttgtgacagg gtcacaaatc ccgctggctg agttacgctc tgacggacaa
    38161 attaatctgc tgaatgcgtt gtacgttgcg gcgaattatc cgatcaacga agtaacgctc
    38221 tttttcaata accgattgta tcgcggcaac cgcactacca aagcccatgc cgatggtttt
    38281 gatgcgtttg cctctccaaa ccttcctccg ttactggaag ctggtatcca tattcgtcgt
    38341 ttgaatacgc cacccgcccc gcacggtgaa ggggaattaa tcgttcatcc aatcacgcca
    38401 caaccaattg gcgtagtgac gatttatcca gggatttctg ctgacgtcgt gcgcaatttt
    38461 ctgcgccaac cggtgaaagc attgattctg cgctcctatg gcgtgggtaa tgcgccacaa
    38521 aacaaagcct tcctgcagga attacaagaa gccagcgatc gcggtattgt ggtggtcaac
    38581 ctgacacaat gtatgtccgg taaagtgaat atgggtggtt atgccaccgg taacgccctc
    38641 gcccatgccg gcgttattgg cggtgcagat atgactgtag aagccacgct aaccaaactg
    38701 cattacctgc tgagccagga actggatact gaaaccattc gcaaggccat gagccaaaac
    38761 ctgcgcggcg aactgacgcc ggatgattaa ggagactgca atgccccatc gcgccctgtt
    38821 actggtcgat ttacaaaatg atttctgtgc tggtggcgcg ctcgccgtgc cggaaggtga
    38881 cagtacggta gatgtcgcta accgcctgat tgactggtgc cagtcgcgcg gtgaagcggt
    38941 tatcgccagt caggactggc acccggcgaa tcacggcagt tttgccagtc agcacggtgt
    39001 agagccttat acgccaggcc aactcgacgg tttgccacaa accttctggc cagatcactg
    39061 tgtgcagaac agtgaaggcg cacaattaca tccgttactg aaccaaaaag cgatcgcagc
    39121 ggtgttccat aaaggtgaaa atcctttagt tgacagttac agtgcctttt ttgataacgg
    39181 ccgtcggcag aaaacctctc tcgatgactg ggtacgcgat catgaaatcg atgaattgat
    39241 cgttatgggc ctggctactg actattgcgt gaagtttacc gtgctggacg cgttacagtt
    39301 aggttataag gtaaacgtga ttaccgatgg ttgtcgtggc gtgaatatcc agccccagga
    39361 cagtgcgcac gcgtttatgg agatgtcagc agctggggca acgctatata cgctggcaga
    39421 ctgggaagag acgcaggggt aatattagtt gaattcatct ctggcctaca tttcctcaat
    39481 gacattgtag gccagataat acttgtcagt gttgcattta acctacttgc agctaaaaca
    39541 cgacgtccta acaagtatat ttgccctccc ttaataacag gcttaaatac tagataactc
    39601 attcacttaa acatagcaat aggctttgct gagataccaa aatcttcttt taactgctgt
    39661 ttgctcttca tgaccatctg accatttgtg tcaattgtca tatgctgtgc ttcggtatta
    39721 tggcgggctt gccacagcat caccagttgc aggcaattct ctttttgttc ctcggtcaac
    39781 gcaacgccat caggccattt ccccagttca acggcggtcg acaaacgctg gtatacctca
    39841 ggcgtcatgc tgttgataat gtcatcaaga ttcataattt ttccgctcct gtggaataag
    39901 ttgctgaatc gttttttcaa ccgttgattt cttcgccgtt ttcgccatca gtgaagcgta
    39961 aagaagcaga gttaacacaa taacgttcgc ctgttggttg cggcccgtcg ggaaagacat
    40021 gccccagatg ggcatcacag ttaccgcaac gaatttctat gcgctgcatt ccatgtgaca
    40081 agtctttgat ataacgaatg gattcttcac tcaccggttc gtagaaactg ggccagccac
    40141 agccggaatc atacttggtt tcggaatgaa acagcggggc atcgcagatc aaacagtgat
    40201 atacgccgtc acgcttgtta tgcagtaaac gacccgtaaa tggcggttct gtcccatgat
    40261 tctgcgtcac gtaaaactgc atctcggaca aatttttttt cagttcttct gccgaaggtt
    40321 tattagccat ttgctcacat ctcactttaa tcgtgctcac attacgtgac tgattctaac
    40381 aaaacattaa caccaactgg caaaattttg tcctaaactt gatctcgacg aaatggctgc
    40441 acctaaatcg tgatgaaaat cacattttta tcgtaattgc cctttaaaat tcggggcgcc
    40501 gaccccatgt ggtctcaagc ccaaaggaag agtgaggcga gtcagtcgcg taatgcttag
    40561 gcacaggatt gatttgtcgc aatgattgac acgattccgc ttgacgctgc gtaaggtttt
    40621 tgtaatttta caggcaacct tttattcact aacaaatagc tggtggaata tatgactatc
    40681 aaagtaggta tcaacggttt tggccgtatc ggtcgcattg ttttccgtgc tgctcagaaa
    40741 cgttctgaca tcgagatcgt tgcaatcaac gacctgttag acgctgatta catggcatac
    40801 atgctgaaat atgactccac tcacggccgt ttcgacggta ccgttgaagt gaaagacggt
    40861 catctgatcg ttaacggtaa aaaaatccgt gttaccgctg aacgtgatcc ggctaacctg
    40921 aaatgggacg aagttggtgt tgacgttgtc gctgaagcaa ctggtctgtt cctgactgac
    40981 gaaactgctc gtaaacacat caccgctggt gcgaagaaag tggttatgac tggtccgtct
    41041 aaagacaaca ctccgatgtt cgttaaaggc gctaacttcg acaaatatgc tggccaggac
    41101 atcgtttcca acgcttcctg caccaccaac tgcctggctc cgctggctaa agttatcaac
    41161 gataacttcg gcatcatcga aggtctgatg accaccgttc atgctactac cgctactcag
    41221 aaaactgttg atggcccgtc tcacaaagac tggcgcggcg gccgcggcgc ttcccagaac
    41281 atcatcccgt cctctaccgg tgctgctaaa gctgtaggta aagtactgcc agaactgaat
    41341 ggcaaactga ctggtatggc gttccgcgtt ccgaccccga acgtatccgt agttgacctg
    41401 accgttcgtc tggaaaaagc tgcaacttac gagcagatca aagctgccgt taaagctgct
    41461 gctgaaggcg aaatgaaagg cgttctgggc tacaccgaag atgacgtagt atctaccgat
    41521 ttcaacggcg aagtttgcac ttccgtgttc gatgctaaag ctggtatcgc tctgaacgac
    41581 aacttcgtga aactggtatc ctggtacgac aacgaaaccg gttactccaa caaagttctg
    41641 gacctgatcg ctcacatctc caaataagtt gagatgacac tgtgatctaa aaagagcgac
    41701 ttcggtcgct ctttttttta cctgataaaa tgaagttaaa ggactgcgtc atgattaaga
    41761 aaatttttgc ccttccggtc atcgaacaaa tctcccctgt cctctcccgt cgtaaactgg
    41821 atgaactgga cctcattgtg gtcgatcatc cccaggtaaa agcctctttt gcattacagg
    41881 gcgcacacct tctctcgtgg aaacctgcgg gtgaagaaga agttctgtgg ttgagcaaca
    41941 acacaccgtt caaaaatggc gtcgctattc gcggtggcgt accggtttgc tggccgtggt
    42001 ttggtccggc ggcacaacaa ggtctgcctg cgcacggttt tgcccgcaac ctgccgtgga
    42061 cgctgaaatc acatcgtgaa gatgctgatg gcgtagcgct gacttttgaa ttgacacaaa
    42121 gcgaagagac gaaaaaattc tggccgcacg actttacgct gttagcgcat ttccgcgtgg
    42181 gtaaaacctg tgaaatcgat cttgaatcac atggcgaatt tgaaaccacc tctgccctgc
    42241 atacctactt taacgtgggt gatatcgcta aggtaagcgt cagtgggctg ggcgatcgct
    42301 tcattgataa agtaaatgac gcgaaagaag atgtactgac cgacggtatt cagaccttcc
    42361 ctgaccgtac cgatcgcgtg tatctgaatc cgcaagattg cagcgtgatt aatgatgaag
    42421 cgctgaatcg tattatcgcc gtaggccacc agcatcatct gaacgttgtc ggctggaacc
    42481 cgggaccggc gctttcagtt agcatgggcg acatgccgga tgatggctac aaaacatttg
    42541 tttgtgtaga aacggcttac gcttcagaaa cgcaaaaagt gaccaaagag aaacctgcac
    42601 atctggcgca atccattcgc gttgcgaaac gttaatttgc gttaatgttg tgtgccgggt
    42661 gcgatgcatc cggcacgtat caacatcaca ccatatccag cgcagttttt ccttttggtg
    42721 ccggatacgc tttatccagc atggttaatt ccgctgacga aagttcgacc tcaagcacag
    42781 ccgcattctg ttggacatgg gcaatcgtgg ccgcttttgg aatcgccatc acaccctgat
    42841 ggctgattac ccacgccaac aatacttgtg ccgcgctgat attgtgagca tgtgcaattt
    42901 cgttgactac cgcgtttttt aacagtccat tgcgcaaccg cccggcctgg gctaatggac
    42961 tgtaagccat caccggcatc tgctgttgct ggcaccaggg gagtagatcg tactcaatcc
    43021 ctcgtgaacc gagatggtaa agcacctgat tagtggcaca ctgatttccc cccggcagct
    43081 gccagagttc ctgcatatca gcatgatcaa ggttagaaac gccccagcgg cggatttttc
    43141 cctgggcgat caatttttcc atcgctgcca cagtctcttc aaaagcgaaa ctgccagacc
    43201 agtgtaataa gtagagatcg agataatcag tattgagacg gcgtaaactg gcttcgcatg
    43261 catttatcgc tttttgcccg ccagcattcc acggatagac tttagagacg agaaagacgt
    43321 tatcgcgcag accggttaat gcttccccaa ccaccttttc ggcaccgcca tcggcataca
    43381 tttcggcggt atcaatgagg gttaaaccga gctcaatgcc cgcgcgtagt gcagcaactt
    43441 ctgttttgcg ctgactggca tcttcgccca tataccatgt tccctgccct atggctggca
    43501 gtgagacatc gccactaaat tgaatcattt tttgttgcat tgtttcctcc caggtattgc
    43561 accaccgtaa tgcaaaacag ggcgtgacgc cctgttttta tgcacaaaat gccctggaaa
    43621 gatgcattat cagaatttgt aggtgatccc ggtagaaatc aggccagtcc aggatttatc
    43681 caccatcggg ctgtcagtaa cttcatcaga cagacgggta tagcgcgcgg taccgtaaac
    43741 actccagtcg ccgaggaagt tgtagctggc gctcagctcc aggtaagggc tccagctgtc
    43801 gttcgggtta tagccacgca gaccgctgcg agcggactct ttgcgcgata cgccataata
    43861 gtattcgttc tggttttcgc tgttccactg cacaccgata cccggggtca cggtcaggcc
    43921 gccgttggtg taacgataca accaggccat atcccagacg atgccgttgc tgttatccag
    43981 ggtatcgcca gccagggtgg tacgcaggta accgtactgg gtaaagtgag cataagacag
    44041 accagccatc atggtgctct tacggtcatc caggtgacgc atttggtgat cgccactgtc
    44101 tttcgctttg aagtaaagcg gcgaccaata agcggtaatt gaaagtttgt ccgttgcgtc
    44161 attccacagg tagtaaccac cacctaagcc acggaaccag aagttatcgc cttcatagtt
    44221 gattaccggt actgggtaaa catcggtatc gtaatcttta tatgggtgct caacgacacc
    44281 tacgcctgcg cccagggaaa atttaccttc agcgtgcgct acgcctgcag acgttgcgat
    44341 aagcactcca agtgccagaa gtttgagttt ggtcacaatt aatcattcct taaacaaatg
    44401 tttagcgggc gacaaagttt acccgtcaat acatcgaaac ccaacctttt tacgttttca
    44461 ttttttaaag taactgttta attttcctga cgcggatgac accgcgctta cagccaaatg
    44521 aatttagcgt tactggctac cctggtcttt acattaatta tgcaaaattt atggatgagt
    44581 tgttgatatg ccattgaaat taagaaagcc gtgcaggcaa gttttccatt tgccatctac
    44641 gcttaatttt gaaggtgtat caccgggcac gttgttctca tcgtcgataa aatggcatga
    44701 gagttgctgt gttttagcaa gagacgtcgt tcagtttacc tcttccggga gcctctacta
    44761 ttcatatgaa cggctcttaa cctgtgctaa aaaacgaaag gacggcatac catgaatata
    44821 ttcgatcact atcgccagcg atatgaagct gccaaggacg aagagttcac actgcaggag
    44881 tttcttacca cttgtcggca agatcgcagt gcttatgcca acgcggctga gcggctattg
    44941 atggctatcg gtgagcctgt catggtcgat acagcccagg aacccagact ttctcgactc
    45001 ttttctaacc gggtcattgc acgttatccg gcatttgaag agttttacgg catggaagac
    45061 gcgattgaac agattgtctc ttatctgaaa cacgcggctc aggggctgga agagaagaaa
    45121 caaatcctgt atctgctggg gcctgtgggt gggggtaaat catcgcttgc tgagcgactg
    45181 aaatcattaa tgcagctcgt accgatttat gtattgagcg ccaacggtga gcgtagcccg
    45241 gtcaacgatc atccgttctg tcttttcaat ccgcaggaag atgctcagat tctggaaaaa
    45301 gagtatggca ttcctcgccg ttatctcggc accatcatgt cgccatgggc ggcaaaacgc
    45361 ctgcatgaat ttggtggcga tatcactaag ttccgggtag tgaaggtctg gccgtcaatt
    45421 ctgcaacaaa ttgctatcgc caaaacggaa cccggtgatg agaacaacca ggacatctcc
    45481 gcgctggttg ggaaagtcga tattcgtaaa ctcgaacact acgcgcagaa tgacccggac
    45541 gcctacggct attccggtgc gctgtgccgc gccaaccaag ggatcatgga atttgtcgag
    45601 atgtttaaag caccgataaa agtgctgcat ccgctgttaa ccgccactca ggaaggtaac
    45661 tacaacggga cggaaggtat ctccgccctg ccgttcaacg ggattattct cgcccactcg
    45721 aacgaatccg aatgggtcac tttccgtaat aacaaaaaca acgaagcctt cctcgaccgt
    45781 gtttacatcg tgaaggtgcc gtattgctta cgcatttccg aagagatcaa aatctacgag
    45841 aaattgctta atcacagtga attgactcac gccccctgcg cccctggcac gctggaaacg
    45901 ctgtcacgtt tttccattct ttcgcgcctt aaagagccag aaaactccag catttattca
    45961 aagatgcggg tttatgatgg cgaaagtctg aaagacaccg atcccaaagc caagtcgtat
    46021 caggaatatc gtgactacgc cggtgtcgat gaagggatga acggtctgtc gacgcgtttt
    46081 gcgtttaaga tcctctcccg cgtgttcaac ttcgatcatg tagaagtggc ggcaaacccg
    46141 gtccatctgt tctacgtact ggaacagcag atcgagcgcg agcagttccc acaagagcag
    46201 gcagaacgct atctggagtt cctgaaaggt tatctgatcc cgaaatatgc cgagtttatc
    46261 ggcaaagaga tccagacggc ctaccttgaa tcctattccg aatatgggca aaacattttc
    46321 gaccgttatg ttacctacgc ggatttctgg attcaggatc aggagtatcg cgatccggat
    46381 accgggcagc tgtttgaccg cgagtctctt aacgccgagc tggagaaaat cgagaaaccg
    46441 gcggggatca gtaatccaaa agatttccgc aacgagattg ttaacttcgt actgcgcgcc
    46501 agagcgaata acagcggacg caatccgaac tggaccagct atgaaaaact gcgcacggtt
    46561 atcgagaaga aaatgttctc caataccgag gagctgttgc cggttatctc gtttaacgcc
    46621 aaaacgtcaa ccgacgagca gaagaaacac gacgactttg tcgaccgtat gatggaaaaa
    46681 ggctacaccc gtaaacaggt gcgtttactg tgcgaatggt atttgcgcgt acgtaaatcg
    46741 tcttaacaac cctggcccgg tacacatgtt accgggccta caacgacagc gaaccgtggg
    46801 cctgagaagc ggcaacacag gcgtagcata cagttggcaa atgtagtacg gggggcatat
    46861 gacctggttt attgaccggc gtctgaacgg caaaaacaaa agcatggtga atcgccagcg
    46921 ttttttacgc cgttataaag cgcaaattaa acagtcgatc tccgaggcca ttaataagcg
    46981 ttcggtgact gacgtcgaca gcggcgaatc cgtatccatt cccacggaag atattagcga
    47041 accgatgttt catcaggggc gtggcggtct gcgccaccgc gtgcatccgg gcaatgacca
    47101 tttcgtccag aacgaccgaa ttgaacgtcc ccagggtggc ggcggaggtt ccggcagtgg
    47161 tcagggccag gccagccagg atggtgaagg tcaggatgaa tttgtctttc agatttcgaa
    47221 agatgagtat cttgatttgc tctttgaaga tttggcctta ccgaatctga aacaaaacca
    47281 acaacgccag ctgaccgaat ataaaacgca tcgggcgggt tataccgcta acggcgttcc
    47341 ggcaaatatc agcgttgtgc gctccttgca gaactcactg gcgcgacgca cagccatgac
    47401 ggcaggcaag cggcgggaac ttcatgcact ggaagagaat ttggccatca tcagcaacag
    47461 tgaacctgcg cagctgctgg aagaggaacg tctgcgcaaa gaaattgcag aattacgtgc
    47521 caaaattgaa cgcgtccctt ttattgacac cttcgattta cgttacaaga actacgagaa
    47581 gcggcccgat ccctccagcc aggcagtgat gttttgcctg atggacgttt ccggttcaat
    47641 ggatcaatcc actaaagata tggctaagcg tttttatatt ctgctgtatc tgttcctcag
    47701 cagaacgtat aagaacgtgg aagtcgtata catccgccat catacccagg cgaaagaagt
    47761 cgatgaacat gagtttttct actcgcagga aacaggcggc accatcgttt ccagcgccct
    47821 gaaactgatg gatgaggtag tgaaagagcg ttataacccg gcacagtgga atatttacgc
    47881 tgcacaagca tcggacggcg ataactgggc cgatgactct ccgctttgcc atgaaatcct
    47941 ggcgaaaaaa ttattacctg ttgttcgtta ttacagctat atcgaaatta cccgtcgtgc
    48001 acatcagaca ttgtggcgag aatatgagca tctgcaatct actttcgaca actttgcgat
    48061 gcagcacatc cgcgaccagg atgatattta tccggtgttc cgtgaactgt ttcataaaca
    48121 aaatgcaaca gctaaagact aaaactatca gccaggtcat tatcgcctgg ctgattttta
    48181 gcttactgta aattatctca tttattacat acat
//
LOCUS       DAZGSP010000027        48168 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZGSP010000027 DAZGSP010000000
VERSION     DAZGSP010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.027"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            114..830
                     /locus_tag="ACHK74_001696"
     CDS             114..830
                     /locus_tag="ACHK74_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0220537.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            860..1360
                     /locus_tag="ACHK74_001697"
     CDS             860..1360
                     /locus_tag="ACHK74_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFP0220538.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1452..4136
                     /locus_tag="ACHK74_001698"
     CDS             1452..4136
                     /locus_tag="ACHK74_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFP0220539.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSYSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKVFSEQDEDSQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4133..5221
                     /locus_tag="ACHK74_001699"
     CDS             4133..5221
                     /locus_tag="ACHK74_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFP0220540.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5309..6607)
                     /gene="exuT"
                     /locus_tag="ACHK74_001700"
     CDS             complement(5309..6607)
                     /gene="exuT"
                     /locus_tag="ACHK74_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFP0220541.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            7090..8502
                     /gene="uxaC"
                     /locus_tag="ACHK74_001701"
     CDS             7090..8502
                     /gene="uxaC"
                     /locus_tag="ACHK74_001701"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP0220542.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8517..10004
                     /gene="uxaA"
                     /locus_tag="ACHK74_001702"
     CDS             8517..10004
                     /gene="uxaA"
                     /locus_tag="ACHK74_001702"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFP0220543.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10087..10638
                     /gene="ygjV"
                     /locus_tag="ACHK74_001703"
     CDS             10087..10638
                     /gene="ygjV"
                     /locus_tag="ACHK74_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFP0220544.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10643..11887)
                     /gene="sstT"
                     /locus_tag="ACHK74_001704"
     CDS             complement(10643..11887)
                     /gene="sstT"
                     /locus_tag="ACHK74_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFP0220545.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12286..13251)
                     /gene="alx"
                     /locus_tag="ACHK74_001705"
     CDS             complement(12286..13251)
                     /gene="alx"
                     /locus_tag="ACHK74_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFP0220546.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(13534..14520)
                     /gene="ygjR"
                     /locus_tag="ACHK74_001706"
     CDS             complement(13534..14520)
                     /gene="ygjR"
                     /locus_tag="ACHK74_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP0220547.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14599..15282)
                     /gene="ygjQ"
                     /locus_tag="ACHK74_001707"
     CDS             complement(14599..15282)
                     /gene="ygjQ"
                     /locus_tag="ACHK74_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFP0220548.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(15359..15862)
                     /gene="ygjP"
                     /locus_tag="ACHK74_001708"
     CDS             complement(15359..15862)
                     /gene="ygjP"
                     /locus_tag="ACHK74_001708"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFP0220549.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15947..17083
                     /gene="rlmG"
                     /locus_tag="ACHK74_001709"
     CDS             15947..17083
                     /gene="rlmG"
                     /locus_tag="ACHK74_001709"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFP0220550.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            17274..17357
                     /locus_tag="ACHK74_001710"
                     /pseudo
     CDS             17274..17357
                     /locus_tag="ACHK74_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            17354..17770
                     /gene="higA"
                     /locus_tag="ACHK74_001711"
     CDS             17354..17770
                     /gene="higA"
                     /locus_tag="ACHK74_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFP0220551.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17815..19833)
                     /gene="fadH"
                     /locus_tag="ACHK74_001712"
     CDS             complement(17815..19833)
                     /gene="fadH"
                     /locus_tag="ACHK74_001712"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFP0220552.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPAGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(20159..22510)
                     /gene="ygjK"
                     /locus_tag="ACHK74_001713"
     CDS             complement(20159..22510)
                     /gene="ygjK"
                     /locus_tag="ACHK74_001713"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP0220553.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIADEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22527..23597)
                     /gene="ygjJ"
                     /locus_tag="ACHK74_001714"
                     /pseudo
     CDS             complement(22527..23597)
                     /gene="ygjJ"
                     /locus_tag="ACHK74_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(23731..25164)
                     /gene="ygjI"
                     /locus_tag="ACHK74_001715"
     CDS             complement(23731..25164)
                     /gene="ygjI"
                     /locus_tag="ACHK74_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0220554.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(25227..25676)
                     /gene="ebgC"
                     /locus_tag="ACHK74_001716"
     CDS             complement(25227..25676)
                     /gene="ebgC"
                     /locus_tag="ACHK74_001716"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFP0220555.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(25673..28765)
                     /gene="ebgA"
                     /locus_tag="ACHK74_001717"
     CDS             complement(25673..28765)
                     /gene="ebgA"
                     /locus_tag="ACHK74_001717"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFP0220556.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(28949..29932)
                     /gene="ebgR"
                     /locus_tag="ACHK74_001718"
     CDS             complement(28949..29932)
                     /gene="ebgR"
                     /locus_tag="ACHK74_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFP0220557.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            30151..30483
                     /gene="ygjH"
                     /locus_tag="ACHK74_001719"
     CDS             30151..30483
                     /gene="ygjH"
                     /locus_tag="ACHK74_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFP0220558.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(30525..31904)
                     /gene="ygjG"
                     /locus_tag="ACHK74_001720"
     CDS             complement(30525..31904)
                     /gene="ygjG"
                     /locus_tag="ACHK74_001720"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFP0220559.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            32322..33842
                     /gene="aer"
                     /locus_tag="ACHK74_001721"
     CDS             32322..33842
                     /gene="aer"
                     /locus_tag="ACHK74_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFP0220560.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLS
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(33996..34619)
                     /gene="nfeR"
                     /locus_tag="ACHK74_001722"
                     /gene_synonym="yqjI"
     CDS             complement(33996..34619)
                     /gene="nfeR"
                     /locus_tag="ACHK74_001722"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFP0220561.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLHVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            34896..35660
                     /gene="nfeF"
                     /locus_tag="ACHK74_001723"
                     /gene_synonym="yqjH"
     CDS             34896..35660
                     /gene="nfeF"
                     /locus_tag="ACHK74_001723"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFP0220562.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(35714..35789)
                     /locus_tag="ACHK74_001724"
     tRNA            complement(35714..35789)
                     /locus_tag="ACHK74_001724"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35754..35756),aa:Ile,seq:cat)
     gene            35914..36420
                     /gene="mug"
                     /locus_tag="ACHK74_001725"
     CDS             35914..36420
                     /gene="mug"
                     /locus_tag="ACHK74_001725"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFP0220563.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(36499..38340)
                     /gene="rpoD"
                     /locus_tag="ACHK74_001726"
     CDS             complement(36499..38340)
                     /gene="rpoD"
                     /locus_tag="ACHK74_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFP0220564.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(38535..40280)
                     /gene="dnaG"
                     /locus_tag="ACHK74_001727"
     CDS             complement(38535..40280)
                     /gene="dnaG"
                     /locus_tag="ACHK74_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP0220565.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQALAKK"
     gene            complement(40391..40606)
                     /gene="rpsU"
                     /locus_tag="ACHK74_001728"
     CDS             complement(40391..40606)
                     /gene="rpsU"
                     /locus_tag="ACHK74_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFP0220566.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            40844..41857
                     /gene="tsaD"
                     /locus_tag="ACHK74_001729"
     CDS             40844..41857
                     /gene="tsaD"
                     /locus_tag="ACHK74_001729"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFP0220567.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(41900..43363)
                     /gene="ttdT"
                     /locus_tag="ACHK74_001730"
     CDS             complement(41900..43363)
                     /gene="ttdT"
                     /locus_tag="ACHK74_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFP0220568.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(43412..44017)
                     /gene="ttdB"
                     /locus_tag="ACHK74_001731"
     CDS             complement(43412..44017)
                     /gene="ttdB"
                     /locus_tag="ACHK74_001731"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFP0220569.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(44014..44925)
                     /gene="ttdA"
                     /locus_tag="ACHK74_001732"
     CDS             complement(44014..44925)
                     /gene="ttdA"
                     /locus_tag="ACHK74_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFP0220570.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            45132..46064
                     /gene="ttdR"
                     /locus_tag="ACHK74_001733"
                     /gene_synonym="dan"
     CDS             45132..46064
                     /gene="ttdR"
                     /locus_tag="ACHK74_001733"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFP0220571.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(46077..46694)
                     /gene="plsY"
                     /locus_tag="ACHK74_001734"
     CDS             complement(46077..46694)
                     /gene="plsY"
                     /locus_tag="ACHK74_001734"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFP0220572.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            46799..47167
                     /gene="folB"
                     /locus_tag="ACHK74_001735"
     CDS             46799..47167
                     /gene="folB"
                     /locus_tag="ACHK74_001735"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFP0220573.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPSAV
                     ARAANVGVIIERGNNLKENN"
     gene            47257..48078
                     /gene="bacA"
                     /locus_tag="ACHK74_001736"
     CDS             47257..48078
                     /gene="bacA"
                     /locus_tag="ACHK74_001736"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP0220574.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHETIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
BASE COUNT        11687 a        12256 c        12714 g        11511 t
ORIGIN      
        1 cccagccttt aaatttaata ttaactaaaa atgctttttt atattgactt acaataaatc
       61 tacatctcta atacaccaac tttttggaaa caatattgta agatcaaatt tatatggtgg
      121 cgaaaattaa tctcttatca cggcttacgc cgcttttatt cgtgttcgct ccttttttgg
      181 cacagtcaaa tatgactgtt tatcctatgg ctgtttccat taatagtcag ggtgaaggca
      241 acgttcgcgt aatatctaaa agtaatgaag ttcagtacat caaggcgaca gtattccgta
      301 tcgataatcc atcgacgcct caggaaaatg aagttgagat taaatccggt gatgcaaatc
      361 atctggttgt tatgccacct aaattcgctt taccagccgg tagtagcaaa accgtacgtt
      421 ttgttgcgat ggaaccagag caaaaagaga aaaattatcg cgttaaattt gaagcggttc
      481 ccagtattga tgacgttgcc acagataaaa aagatctctc tatgcagtta acagttaact
      541 taatttgggg gattgttgtt agtgttccac ctcagcaacc tattgctaag ttagaagtaa
      601 atgctgccca aaaattagtt aatgcaggga atcaacgctt aaagatttta acgatcgctt
      661 attgtaaaaa taatagcaaa gaaaattgta agatacagac cgtaaataaa aacatcttcc
      721 ctggtcagga aagaaatctt gaaagcatat ctggctacga caaaatcgtt gtcaaatata
      781 ataactggat caccaaagat aatggcgagt ttgaactggc ggtccattaa ttatcattgt
      841 aataaacgaa ggaatagtaa tgaaaaaggt atttgcaaaa tctcttctgg tcgcagcgat
      901 gttttctgtt gcaggctccg cattggcagt acaaaaggat attactgtaa cggccaacgt
      961 tgatgccgct ctggatatga cacagaccga taacaccgcg ttgccgaaag cagtagaaat
     1021 gcaatatctg ccggggcagg gtctgcaatc ttaccagttg atgaccaaaa tctggtctaa
     1081 cgacgttacc aaagatgtaa aaatgcagtt agtttccccg gcgcaactgg ttcaaagcct
     1141 ggatgctagc aagattgttc cgctgacagt aacctggggc ggtgaagaaa ttaaagctga
     1201 tgcggcaacc acttttaccg caaccaaaat ctttgcttcc gatgcgttaa ctaacggttc
     1261 tctggctaaa aacctgatgt ttgctcaaac caccaaaggt gttctggaga caggcatcta
     1321 ccgtggtgta gtaagcattt atctgtctca ggatatctaa tccagataac agaattaaca
     1381 cgtaacaaag ggaggggtag ccctccctta ttttaactgt tttagtattt gttatggatt
     1441 tcaggattgc catggataaa aaattactgg ctcttttgat cctggcgagt ctcagcccgg
     1501 cagaggcgac attaaccaaa attcccgcag ggtttgaggt tattgctcag ggacagcagg
     1561 agtatatcga ggtttatttt tcagggaaaa gtctcggtaa atattatgca atggttaatc
     1621 ttgataccgt aacatttctt gatccagcaa gtctatataa caagctggaa ctggatgtag
     1681 acgatcagaa aatcgcgcat atagtgaaag aaaaattatc gcagccgcta gctcgccacg
     1741 gtgaattggc ttgcggttat gtacgtactg actcagggtg tggttttctg aataccgata
     1801 cgctggaaat aatctataat gatgaagaaa gttcggcaac gttgtttatt aatccgcaat
     1861 ggaattcagc tttcgatgcg aagtcattat atttaaatcc agacaaaaat actgttaatg
     1921 cttttataca tcagcaagac atcaatgttc tggcacagga tgattaccaa tcgttgtcta
     1981 ttcagggaaa cggtgcgctg ggaataacag aaaatagtta tattggtgca cactggaatt
     2041 tcaacggtta tgatgcagat gatgtcagtg acagtaatgc tgacgtcagc gatctctatt
     2101 atcgttatga ttttttacgt cgttattatg tgcaggcggg tcgcatggac aaccgcacac
     2161 tatttaatgc acaaggcggg aactttacct ttaactttct gccactcggt gcaattgatg
     2221 ggatgcgtat cgggtcgaca ctgagctatt taaaccaggc gcaaagccag caaggaaccc
     2281 cagtaatggt tctgctttcg cgcaattctc gtgttgacgc ttatcgtaat gagcaacttt
     2341 tgggatcgtt ttatctcaat agtggttcgc aatttattga taccagttcc tttccgcctg
     2401 gcagctacag cgtggcatta aaagtctacg aaaataacca actcacccgc accgagcttg
     2461 tgccgtttac caaaaccggc ggtctgactg acggaaatgc gcaatggttc ttacaggcag
     2521 gtaaaactac atcacaggtt tctgatgatg aaagttcagc ttatcagcta ggagtacgcc
     2581 tgccattaca tccgcaatat gagctctacg cagggctggc gaatgccgat gatgtgagtg
     2641 ctttcgagtt aggtaataac tggacggcag atttaggcgg ggtggggaat cttgcaatca
     2701 gcgccagcgt gttccgtaac gatgacggcg gcaaaggtga tatgcaacag gccaactgga
     2761 gtaatccggg atggccgacg ttgggctttt atcggaccaa ctctgacggc gatgcttgta
     2821 caaccgacag cagagagagc tataacgcct taagctgtta tgaaagtatt tccgcgacgg
     2881 tttcacagaa ttttgtcggc tggaatatga tgctgggtta tacccgcaca caaaataaca
     2941 ctgatgatag tttgcgttgg gataaacagc agagctttga aaataactat cttcgccaga
     3001 caacagcgca aagtatctct gaaactgtac aacttagcgc ttcccgcgct tttgtgatgc
     3061 gtgactggat tttgagtacg tcagtcggtg ttttccatcg taatgacaac ggtggcgata
     3121 acgacgacaa cggcttgtac ttatcgtttt cgttatctga cacgccaacg atggatagca
     3181 ataacaacag ctattcaaca aatgtgtcta cggattatcg ttatagcgaa caggatggcg
     3241 atcaaacgtc atggcagtta tcgcatacct tttataacga ttcattcagc cataaagaac
     3301 ttggagtaac cgtcggaggc ctgaacaccg ataccataaa cagcgcggtt aacgggcgtt
     3361 gggatgggca atacggaaat gtctacgcta ccgtatctga tagttatgac cgtaagaatc
     3421 atgatcatct ttcggccttt acgggcactt atagctctac gctggctgtc agtcgctatg
     3481 gcgttaattt gggagccagt ggtacagacg atttgctggg tgcggtactg gtggatgtga
     3541 aagtcttctc tgaacaggat gaagatagtc aggatctgca actcgaagcg cgggtggcag
     3601 gcagccgaac tttgcagctt ggtcaaagtg acagtgtgtt gttcccttat cctggatttc
     3661 agtctggttt tgttgaggtt aacgacagta gccagggcaa tcagcagggg acaacaaaca
     3721 tcattaacgg tgcagggaat cgtgaattaa tgttgttgcc tggcaagctg cgctatcgcg
     3781 aagtgtctgc cagctttaac tacaactata tcggtcgttt attactgccg gcagcggtga
     3841 aaaaattccc gattgtgggg ctgaacagcg ccatgttact ggtagctgaa gatggcggat
     3901 ttacacttga aattaacggc agcgaaaaag agctctatct gctttccggg cagcaattcc
     3961 ttaagtgtcc actgagtgtt gtaaagaaac gcgccagcat tcgttacagc ggagatgtta
     4021 cttgtagtgt ggtgacttat tcacaattac cggagtcgat tcaggttcag gcacagttga
     4081 aacagcctaa attacgtgga aacgttcaga cggcgcaaag ggaggttgca ccatgagaaa
     4141 tcgattgatt gcggcgatat tgggcttgtt tggcacgctc actggcgttc aggcagctcc
     4201 tgacgtgacc agtgaaatta cgtatgattt ggcatctggt agagcggatt attacttctg
     4261 gaaggatgaa gcgtcggcag gaaataatgg atatatgtgg tatgaatgtt cgtatcctga
     4321 cctccaacaa acctgtacag ctaatggaaa tatatcgaca gtacaaatct atttaactga
     4381 acaacgcagt gggatgcgtt ggccggtaaa actcaaagga tttaaaacag ccattgtaag
     4441 tagcgatgaa gcgccgccag gatgcaaggg gggcaaaggg cttcagacga atcttaagga
     4501 ttctaataga tcttcatgta cagaagatgg tcaacattat tatatatacg atacaaagtt
     4561 tcttacgctc taccttgagc agacagagat gaagaatttg ccgattggtg gcgtctggaa
     4621 ggggaaagtt aaattacatt cgaacagccc ggcccaggac tatttcgcaa atattaccct
     4681 gaatacgctc gaccccaacc atattgacgt gttcttcccg gagttcgccc acgccacgcc
     4741 aagggtgcag ttagacttgc atccaacagg aagcgttaac ggcagcaact acgcgcaaga
     4801 tctgaccatg ttggacatgt gcctgtacga tggttttaac ggtaatgcca tcagttatga
     4861 aatcatgctc aaagatgaag ggcgaccagc tgcagggcgc agagacggtt acttctctat
     4921 ctatcgtcag ggagggacca ccaccgacga gggagaacgc attgattacc gggtcaaaat
     4981 gtacaacccg gaaaccggtg ggcaaattga tgtgcgcaat aatgaaaata tggtctggaa
     5041 cagcattaac ctgaaacgtg tgcgtccggt ggtactgccc ggtattcgct atgccgtgat
     5101 gtgtgtgccg acacccttga ctctggcagt cgataaattc agcgtgatgg ataaacaggc
     5161 cgggtactac atgggcaaat tgtcagtaat ctttacgcct tccttgccaa ccatcaatta
     5221 accctgcaaa ccggacaaac gcttttttct atataaacga cgaagccgtc tgataaggcg
     5281 gcttcgtcat tacaacttaa gttaaacgtt aatgttgcgg tgcgggatcg ttatgtgttt
     5341 cctgcgccac ctcaatcgcc ggtttgttct gcaacacggt ccagataacc agcgcaccta
     5401 acaggtcaaa tactgccaga actgcgaaca gcgggctgaa gccgatggtg tcagccagtg
     5461 caccgacaac cagcgcaaac agcgtacttg ccagccatgc ggacatcccg gttaaaccgt
     5521 tcgccgtcgc cacttcgtta cgaccgaaca cgtcagaaga gagcgtaatc agcgcgccag
     5581 acagtgcctg gtgggcaaaa ccaccgatac acagcagcat aattgcgaca tacgggttgg
     5641 tgaacagacc gatcataccc gggccaatca tcagcacagc acccagcgtt acgaccatct
     5701 tacgggaaac gatcaggttc acaccaaacc aacgctggaa cagcggcggc aggtaaccac
     5761 cgaggataca accgaggtca gcaaacagca tcggcatcca ggcgaacatc gcgatctctt
     5821 tcaggttaaa gccgtaaact ttaaacatga acagcgggat ccacgcgtta aaagtacccc
     5881 aggccggttc tgccaggaaa cgcggcagcg cgatacccca gaactgacgg ttacgcagga
     5941 tctgaccaac ggacattttc ttcgccgtgc tcacctggtg ctgggcttcc tgaccattaa
     6001 taatatagtc gcgttcttca tcggtcagat gcttctggtc gcgtggatgt ttatagaaaa
     6061 tcagccatgc catcgcccag ataaagctca atgcaccgga gatgataaat gccatctgcc
     6121 agctgtgcat tacgattgcc cataccacca gcggcggcgc aatcatcgca ccaatcgaag
     6181 aacctacgtt aaagtaacct accgcgatgg aacgctcttt cgccgggaac cattcggagc
     6241 tggctttcag acccgccggg atcatcgctg cttccgcggc accgactgca ccacgagcaa
     6301 tcgccaggcc accccagcta ccagccagcg cggttgcacc acagaacacg gcccacagca
     6361 cagcaaacat tgcataaccg attttcgtac ccagcacatc cagtacatag cccgctaccg
     6421 gttgcatgac cgtataagca gcagaatagg ctgcgatgat ataggaatac tgttgggtgg
     6481 agatgtttaa ctcttccatc agagttggcg cagctgccgc cacagtgtta cgcgtcaggt
     6541 aaccaagcac ggtgccaagc gtcaccagtg cgatcatata ccaacgtaac cctttaattt
     6601 tacgcatgta aaacctcatc gttttgttat atccgcagga agcgcggcca tcatcccgcc
     6661 agaggctgaa gatgttagtt acgaccgggt aaccgaaaaa aaaccggcac gccccgaacg
     6721 ttgccatgaa agtttcgtgc taattcggct tccgtaccgg ttaacccgat tcatctaaca
     6781 aatgaatgaa tcggcaattt gcattccgtc gacttatgat ttgcgacggc agaaagataa
     6841 cttgtcatac aactttaaaa ggtgagagcc atcacaaatg tgcgaatatt tgtagggaca
     6901 ttacctgacg acagcgaggc cagtactggc gcggcctgca gagagattta ccactttgag
     6961 agtaattttt ttaactacgt ttattgatct aactcacgaa aatatcttcg gactctggaa
     7021 attggtatga taactttgtc agcatcgcac cataagcaag ctagctcact cgttgagagg
     7081 aagacgaaaa tgactccgtt tatgactgaa gatttcctgt tagataccga atttgcccgc
     7141 cgtctgtatc acgactacgc aaaagaccag ccgattttcg attaccattg ccatttgccg
     7201 ccgcagcaga ttgcggaaga ctatcgtttt aaaaacctgt atgacatctg gctgaaaggc
     7261 gatcactaca aatggcgcgc tatgcgtacc aacggtgtgg ccgagcgtct gtgtaccggt
     7321 gatgcgtctg accgtgaaaa atttgacgcc tgggcggcga ctgttccgca cactatcggc
     7381 aacccgttat accactggac gcacctcgaa ctgcgtcgtc cgtttggtat cactggcaaa
     7441 ttgctttctc cgtcaactgc cgatgaaatc tggaacgaat gtaacgaact gctggcgcag
     7501 gataacttct ccgcgcgcgg catcatgcag cagatgaacg tgaaaatggt cggcaccacc
     7561 gatgacccga tcgattctct ggagcatcac gcagagatcg ccaaagacgg ctctttcacc
     7621 atcaaagtgc tgccgagctg gcgtccggac aaagccttca acatcgaaca ggcgaccttt
     7681 aacgactaca tggcgaagct gggcgaagtt tccgataccg acattcgccg ctttgctgac
     7741 ctgcaaactg ccttgaccaa acgtctggat cacttcgccg ctcacggctg taaagtgtct
     7801 gaccatgcgc tggacgtggt gatgtttgct gaagcgaacg aagcggaact ggacagcatt
     7861 ctggcgcgcc gtctggctgg cgaacccctg agcgagcacg aagtggcaca gttcaaaact
     7921 gcggtactgg tgttccttgg tgccgaatat gcacgtcgcg gctgggtaca gcagtaccac
     7981 attggcgcgc tgcgtaataa caacctgcgt cagttcaaac tgctggggcc ggatgtaggc
     8041 tttgactcca tcaacgaccg tccgatggcg gaagagttgt ctaagctgct gagcaagcag
     8101 aacgaagaaa acctgctgcc gaaaaccatt ctctactgcc tgaacccgcg cgataacgaa
     8161 gtgctgggca ccatgatcgg taacttccag ggcgaaggta tgccgggcaa aatgcagttc
     8221 ggttccggct ggtggtttaa cgatcagaaa gacggtatgg aacgtcagat gacccaactg
     8281 gcgcagctcg gtctgctgag ccgctttgtc ggtatgctga ctgacagccg tagcttcctg
     8341 tcatacaccc gtcacgaata cttccgccgc attctgtgcc agatgatcgg tcgctgggtg
     8401 gaagcgggcg aagcaccggc ggacatcaac ctgctgggcg agatggtgaa aaatatttgc
     8461 tttaacaatg cgcgtgacta cttcgccatt gaactgaact aaggtctggg gttgatatgc
     8521 aatacatcaa gatccatgcg ctggataacg tcgcggtcgc tttagcagat ttggctgaag
     8581 gcacagaagt cagtgtcgat aaccagactg ttacgctgcg ccaggatgtt gctcgtggac
     8641 ataaatttgc gttaacggat atcgcaaaag gggccaacgt catcaaatat ggcctgccga
     8701 ttggttatgc attggcggat attgcggcgg gggaacacgt ccacgcccac aatacgcgca
     8761 cgaatctgag cgatctggat cagtatcgct atcaacctga ttttcaggat ctgcctgcgc
     8821 aagcggcaga tcgtgaagtg cagatctatc gtcgcgctaa cggcgatgtc ggggtgcgta
     8881 atgagctgtg gatcctgcca accgtgggct gtgtcaacgg catcgcgcgg caaatccaga
     8941 accgtttcct gaaagagacc aacaacgccg aaggtaccga cggcgtgttc ctcttcagcc
     9001 acacctacgg ctgctcacaa ctgggcgacg atcacatcaa cacccgcacc atgctgcaaa
     9061 acatggtgcg ccacccgaac gcgggcgcag tgctggtgat tggtctgggc tgtgaaaaca
     9121 accaggttgc cgcattccgt gaaacgttgg gcgatatcga tcctgaacgc gttcatttca
     9181 tgatctgcca acagcaggat gatgagatcg aagccgggat cgagcatttg catcagctgt
     9241 ataacgtgat gcgcaacgat aaacgcgagc caggcaaact cagcgaactg aagtttggtc
     9301 tggagtgcgg tggttctgac ggtctttctg gtattactgc taacccgatg ctggggcgtt
     9361 tctctgacta cgtgattgct aacggcggta ctaccgtact gaccgaagtg ccggagatgt
     9421 ttggcgcaga gcagttgctg atggaccatt gccgcgacga agcaacgttt gaaaaactgg
     9481 tcaccatggt caacgacttc aaacagtact ttattgccca tgaccagccg atctatgaaa
     9541 acccatcgcc ggggaacaaa gcgggcggta tcaccacgct ggaagataaa tcccttggct
     9601 gtacccagaa agcgggttcc agcgtcgtgg ttgacgtgct gcgttacggc gagcgtctga
     9661 aaacgccggg gctgaacttg ttaagtgcgc cgggtaacga tgccgtagcg accagcgccc
     9721 tggcgggtgc gggctgccat atggtgctgt tcagtactgg tcgtggcacg ccatacggcg
     9781 gctttgtacc gacggtgaaa atcgctacca acagtgaact ggcggcgaag aaaaaacact
     9841 ggatcgactt tgacgcgggt cagctgatcc acggtaaagc gatgccgcag ttgctggaag
     9901 aatttatcga caccatcgtt gagtttgcca acggtaaaca aacctgcaac gagcgtaacg
     9961 acttccgcga actggcgatc ttcaaaagcg gcgtaacgct ataaagtcga aatgagttgt
    10021 taataaaaaa cggcgtttca taatgtgaag cgccgtttgc ttttccatac ctgataagga
    10081 tttatcatga ccgcgtattg gctggcccag ggcgtgggtg tcatcgcctt tctgattggt
    10141 atcacaacat ttttcaatcg tgacgaacgt cgcttcaaaa agcagctttc ggtctatagc
    10201 gccgttattg gcgtacattt ttttcttctg ggcacctatc ccgctggtgc cagtgccatc
    10261 cttaatgcca ttcgtacatt gattacctta cgcacgcgca gcttatgggt aatggcgatt
    10321 tttattgtgc tgactggcgg aattggcctc gcgaagttcc atcatcctgt cgaactattg
    10381 ccggttatcg gtacgattgt cagtacctgg gcgctgttcc gctgtaaagg gctcaccatg
    10441 cgctgcgtga tgtggttttc aacgtgttgc tgggtgattc acaacttctg ggcggggtcg
    10501 ataggcggca caatgattga gggtagtttt ctgcttatga atggcttgaa tatcattcgt
    10561 ttctggcgga tgcagaaaag gggaattgat ccgtttaaag tagagaaaac ccctcccgcc
    10621 gtagacgaaa ggggttaaac aattaattac gcagggcgct atttgccaga cgatcgtctt
    10681 ccgcctggca agctgccgca gtgaacagca cgtcagttga agagttcagc gcggtttcgc
    10741 aagagtcctg caatacgccg atgataaagc cgacggcaac cacctgcatg gcgatatcgt
    10801 tcgaaatacc gaacatatta caggccagcg ggatcagcag cagagaaccc cccgccacgc
    10861 cggatgcgcc acaggcacac agagaagcca ccacgctcaa cagcagcgcc gtgggcagat
    10921 cgaccgggat acccagcgta ttaaccgcag ccagcgtcaa cacggtaatg gtgattgctg
    10981 cgcccgccat attgatggtg gcacccagcg gaatagaaac ggaataggta tcgcgatcca
    11041 gattcagctt ttcacacagt gccatattca ccggaatgtt cgccgcagag ctgcgggtga
    11101 agaaggcata cacgccgctt tcgcgcaggc acagcagcac cagcgggaac gggttacgac
    11161 gaattttcca ccacaccagc aatgggttaa ccaccagcgc caccagtaac atacagccaa
    11221 ccagcacgac cagcagttgc gcgtagcccc acagtgtgga gaaaccggtg gttgccaggg
    11281 tagaagaaac cagaccaaaa ataccaatcg gtgcgaagcg aatgaccagt ttcaccataa
    11341 aggtgacggc attcgacata tcgttgacca ggtttttggt ggtctcgtta ccgtgacgca
    11401 gtgcgaaacc gaggccaatt gcccacacca ggatcccgat gtagttacct ttcagcagcg
    11461 cgtcgatggg gttggaaacc atgctcatta ccagcccgcg catcacttcg acaatgcctg
    11521 acggcggcga aatatcaccc gcgctactgg ataaatgcag ggtagaaggg aaggcaaagc
    11581 tgaagactac tgcggccaga gcagcagaga aggtacccag cagatagagg aacaaaatag
    11641 ggcggatatt ggttttctgc ccgtgctggt ggttagcaat agatgccatc accagcatca
    11701 acaccaggat gggggcaacg gctttcagtg cgccgacgaa caaagtacct aacagaccaa
    11761 cagcttccgc cgcgggtttt gagatccatg ccagaagaat ccccagaaca aggccgacca
    11821 ggatttgttt taccaggctg ccatgagcca gacgccggaa tagccccggt gaacgttgcg
    11881 tagtcatttt tcgatccttt cattgtgttg tcgcacatcc ctggtgcgtt ctgtcgacgc
    11941 atctttcagg atgtaacaaa gtgtttgcaa ggtcgagtat aaggaaagtt acagcttcgg
    12001 gaagcgaaaa atgctggatt ttaaagagag gtcatatttt gcaacttaat ggttacattt
    12061 atttgacata aaaataaagc caacgtgcgg cttccagact gatgacaaca tcaaaaaagg
    12121 actcggtcag tcccctcgcc ccgtttgggg agagggttag ggtgagggga acaggccagc
    12181 atcggtgcga aaaattaccc tcaccccgac cctctccctg gaagggagag ggagaaaacg
    12241 gtgctcatgt taatgacagg gtacacggct taggcagatt aaaaattatc caccccgctg
    12301 cttatcatgc cgataattca cccaggcgtt gataataaac gtcatcacca gaatgccaaa
    12361 caccacgccc agcgagacgg cgattggaat atggtagaag tcgacaatca gcatcttgat
    12421 accgataaac accagaatca ccgccaggcc atatttgagc atcgagaaac gctctgctac
    12481 gcccgccagc aggaaataca tcgcacgcag gccgaggatt gcaaacaggt ttgaggtcag
    12541 cacaataaac ggatcagtgg tcacggcgaa gatagccgga atgctatcca cggcaaaaat
    12601 cacgtcgctc aattccacca gaatcagtac cagcatcagc ggtgtggcgt acagcaatcc
    12661 attcttacgc acaaagaaat gctcgttgtc gatggtgtcg gtcatgcgca aatgaccgcg
    12721 tagccagcgc accagcggct tgtcgccaat gcctgattca tcttcatggg cgagcgccat
    12781 cttcacgccg gtaaacagca ggaaggcacc gaagatatac aggatccagt cgaactgcga
    12841 aattagccag ctgccagtga agatcatgat ggtacgcaga acaatcgccc cgagcacgcc
    12901 atacaccagc acgcggcgtt gtaatgccgc cggaacagag aaatagctga acaacatcag
    12961 ccagacaaag acgttatcga ccgccagcga tttctcaatc agatagcccg tgagaaaggc
    13021 cagtgcctgt ggatcggcaa cggcgcgacc ttcagtttgc accagatacc accagaaagc
    13081 ggcgttaaac agtaacgaca gcgtcaccca gaccagcgac caggccgcag cctgtttcat
    13141 ggtcatggca tgtgccccac gacgcccctg caacaacagg tcgatagcca gcataatggc
    13201 gacaacaaca gcgaatccgc cccatagcaa cggcgtgccg acagtattca tagaagttcc
    13261 ttacacataa aaaaacggcc aacgtcggaa gacgtcagcc gctgcttttt atgcatagac
    13321 ctcgccttcc ggcaaggtct cacttacaac aaaaaaggaa tacgtctgcg tattcctttc
    13381 gggttgcccg gtgaccggat gtggtttttc acacatcgta atgacgatcg accggcaatg
    13441 aagttactcc cctttgcagg taacaaagta tgacagacca ttcagccagt caatggtctg
    13501 ttacatacct tttacactgt tttactttgg caattatagt tttacgctat ctgccggaaa
    13561 aatcacccca gtctggcggc ggatctcggt cagcagtttg gcggtgatgc gactgaccgc
    13621 cagccccgga tgatccacca gatgctcatc caccagggta gcgaacagct ctgcttcgta
    13681 gagcatggta ttaatatgct gcggctgggt gagatcctgc atttggctgc cacgcggcac
    13741 gaagcacact ttctggcatt cagacagttt ttcaatcacc agcgatcctg cttcgccctg
    13801 aatctcgctc gccaggacag aatcactgac ttttgagtgc tgcaaggtga cgctgaaatc
    13861 accgtaatcc atcaccacca cgccgtgggc atctacaccg cttgccagca aactggcggt
    13921 tgcctgcacg cttttcggct caccaaataa cgccaccgcc gacgccaggc agtaaaagcc
    13981 gatatccata attgaaccgt tagagaatga cggattaaag gtgttgggat tttcaccgtc
    14041 caggtaacgt tgatagcgcg aggaatactg gcaatagttg aaaaagactt tacgcaattt
    14101 gccgactttc ggcagcgcct ggcgcagcaa atgaaagttc ggcaggcagg cggttttaaa
    14161 tgcctcaaac agcaccacct gattttcccg cgcacaggca atggcggcat ccacttccgc
    14221 cagattcgac gccagtggtt tctcgcaaat cacatgaatt ttatggctaa ggaaaagttg
    14281 tgtctgggaa aagtgcaggg cattcgggct ggcaatatac accgcgtcaa tggcatcgct
    14341 ttccgccatc gcttccagcg aggtaaacag atgctcgaca gaaaaatcat tggcgaagtg
    14401 ctgagcctgc tcaaggctgc gggaatatac ggcggttaac ttgtatttac cgctctcatg
    14461 ggcggcctcg acgaactggc gagtgatcca gttcgtacca atcacagcga aacgtatcat
    14521 aacgcgtgga tactccataa agggattctg tcgtcaatgt agcacgcctt tttcatcttc
    14581 gacgtgcgca gatacgcatt attcttttgc cggacagcca tgctcactaa acggcccaat
    14641 catgaccgat ggcccgagat atttcggctg gcgatggagg attttggcgt caatcacggc
    14701 gctgacacga gcgagtttct cccgcagttg agtataaaaa ccgtgcttca tattgaggtc
    14761 aggcgcgtta aaaccgatag cgtcgatgcc gtactgtttt gccagccaga tggcgcgttg
    14821 attatgaaat tcttgcgaga taatggtgat atggttttca ccaaaaacct ttttggcacg
    14881 cactaccgaa tccagcgttg agaatccggc atagtcgcag aagatcactt tcgccggtac
    14941 gcctttagcg atcaaagcct gctgcatacc tgaggcttca tcgtaatttt tacgtccgtt
    15001 atcgccactt accagtagcc atttcacttt tccggcgtgg tacaacgctg ctgcggtatc
    15061 aatacgtcgg gtaaagtagc gattacccgg cctggccccc agcaataagc ccacgttacg
    15121 cgccggaact gcgttgacat cgctccaggt cagctgcttg ctggcgttga ccataacgcg
    15181 atcggcaata aaaatggtcg ctcctgctat aagcaacaac aaacagccaa tcttaagagt
    15241 ccggcgagaa aaacaaatac ggagaagaag gcggggctgc atggtcgtta tccttaacga
    15301 aaggggggag gtcgatttta ggcgctaaag aaagaaaaga taacgcctga catcattttc
    15361 agattttgtc ctgcccaagc gacaactgcg ttagccacag ccgggtgtcg aactcaagct
    15421 ggtggtactg cggctccata tggcagcaca actggtaaaa cgctttgtta tgctctttct
    15481 ccttcaggtg cgccagttcg tgcaccacga tcatacgtaa aaacggttcc ggcgcgttgc
    15541 gaaacacagt agcgacgcgg atctctgctt ttgctttcag cttgccgccc tgcacacgag
    15601 aaacagcggt atgtaacccg agcgcgtttt ttagcacatg gatcttattg tcatacatca
    15661 ctttattgat cggcggggca ttgcgcagaa actgattttt tagatcctga gtatattgcc
    15721 agagggcttt atcggtggcg tagtcgtgtg ttcccggata gcgttttgcc agcacatcgc
    15781 ccagacgctg ttcgttaatc agcgttcgca cctgggaaag tagctgctcg ggataaccct
    15841 gaagataagt aagattgctc ataaccgccc acgaaaaaga gaaaaagggt atactcacgc
    15901 acccttttca ggggcaacgt cgaaatttta tcattcagga gggccgatga gccacttaga
    15961 caacggtttc cgttcactga cactacaacg ttttccggcg acggatgacg ttaacccgct
    16021 acaggcgtgg gaagcggcgg atgaatattt gctgcaacag ttggacgaca cagaaatccg
    16081 cggcccggtg ttgatcctga atgatgcctt tggtgcgtta agctgcgcgc tggcggaaca
    16141 taagccgtac agcattggcg actcatacat cagtgaactg gcgacgcgcg agaatttacg
    16201 cctcaacggg attgatgagt cgagcgtgaa gtttctcgac agcaccgccg actacccgca
    16261 acagccgggt gtggtgctga tcaaagtgcc gaaaacactg gcattgctgg aacagcaact
    16321 gcgtgcgctg cgcaaagtgg tcacgtcgga tacacgtatt attgccggtg ccaaagcccg
    16381 tgacattcac acttccacgc tggagctgtt cgagaaagtg ctcggcccga ccaccactac
    16441 gctggcatgg aaaaaagcgc gcctgattaa ttgcactttc aatgaaccgc cgctggccga
    16501 tgcgccgcag accgttagct ggaagctgga aggtactgac tggactatcc acaaccatgc
    16561 gaatgtcttc tcccgcaccg ggctggatat tggcgcacgc ttctttatgc aacatctgcc
    16621 agagaatctc gaaggtgaga ttgtcgatct cggctgtggt aacggcgtta ttggcctgac
    16681 gctgcttgat aaaaacccgc aggcgaaagt ggtgtttgtc gatgaatcgc cgatggcggt
    16741 cgcttccagc cgaatgaatg ttgaaaccaa catgccagag gcgttggatc gctgcgagtt
    16801 tatgatcaac aacgcgctct ccggagtgga gcctttccgc tttaatgctg tgctctgcaa
    16861 cccgccgttt catcagcaac atgcgctgac cgataacgtc gcctgggaga tgttccacca
    16921 cgcccgccgc tgcctgaaaa tcaacggcga actgtatatc gttgccaacc gtcacctgga
    16981 ttacttccat aaactgaaga agattttcgg caactgcacc accatcgcca cgaataataa
    17041 atttgtggtg ctgaaaacag tgaagctggg gcgtcgtcgg taagattggg agttacctgc
    17101 cggatgcggc gttaacgcct tatccggcct acgttcagca cgttactccc aggccggata
    17161 agacgcggca agcgtcgcat ccggcaatgt ctgcccagtt ctggaatcgc gcttccataa
    17221 aacaaaaaat ccctgtcacc atcctctaca ttctcttgtt tagcgttgtt caacgtttat
    17281 gctacatacg tgaagttatg acgcataaag aatacgattt ctttaccgct gttcatcgta
    17341 ctaaggggaa aaaatgattg cgattgccga catcttgcaa gcaggagaaa agctaactgc
    17401 ggtggcacct tttctggcgg gtattcagaa cgaggaacaa tacacccagg cgctggaact
    17461 ggtagatcat ctgctgctca acgatcctga aaaccccttg ctggatctgg tgtgtgccaa
    17521 aataaccgcg tgggaagaat cagcgcccga atttgcggaa tttaatgcca tggctcaagc
    17581 catgcctggc ggtatagccg tgattcgtac ccttatggat caatatggtt taaccctttc
    17641 cgatctgccg gaaattggca gtaaatctat ggtgtcacgc gttttgagcg ggaagaggaa
    17701 attaacgctg gaacacgcta aaaaattggc aacgcgattc ggcatttctc ccgccttgtt
    17761 tattgattaa cgcgacgtgc cggatgcgtt gcatccggcg cttcaaataa cgatttaaat
    17821 ctccagcgcc agccgtgttc cctgggcaat tgcccgtcgt gcgtccagct ccatagccac
    17881 atcgcagccg ccaattaaat gcacagtttt cccgctatca atcagcggtt gcgccagcgc
    17941 gcggtttggc tcttgccctg cgcagatcac cacattgtcc actgctaata cctgcgtttc
    18001 gccgttgatc accacatgca gcccgtcatc gtcaatcttc tgataactta cgcctgggat
    18061 cattttcaca ccccgcgaga gcagggtggt gcgatggatc cagccggtgg ttttgcctaa
    18121 cccctgtccc ggtttgctgg ctttgcgctg gagcatcaca atttgccgtg ggctacgcgg
    18181 gatctgcatt ccctgcgggc ttaagccacc agcctgttgt aggctgctgt cgatccccca
    18241 ctcattacag aacctggcga tattctggct ggtggattcg cccggctgac ttaaatacat
    18301 cgccgtatca aaaccaatcc cgccacaacc gatgatggca accttgttgc ctaccggcgc
    18361 tttgtcgcgc agtacatcga gataactcaa taccttcgga tgatcgatcc cgtcgatagg
    18421 cggaacgcgc ggcacgatcc cactggcgag gatcgtttca tcgaacgcct gtaactgatc
    18481 cgccgttacg gtgtgattga gtttcagcgt cacgcccgtc acttcgatca tccggtggta
    18541 atagcgcagc gtttcgtaaa actcctcttt gccggggatc tgtttggcga tattaaactg
    18601 cccgccgatc tcgctgtggg catcaaacaa tgttacctga tgcccacgtg ccgccgcgtt
    18661 aatggcaaac gccagcccag caggtcccgc accgaccacc gccagatttt ttttctgcac
    18721 ggcaggaagg attggcattt tggtttcgtg gcaggcgcga ggattcacca ggcacgaggt
    18781 gactttgcca acgaagatct gatcgaggca ggcctgattg cagccaatgc aggtgttgat
    18841 ctcatcggct cgccccgatt gcgcttttga cagcagttcc gcatcagcaa gaaacggtcg
    18901 tgccatcgac accatatcgg catcgccgcg cgagagaatg tcgtcggcaa cctgcggatc
    18961 gttaatccgg ttggtggtca ccagcggcag cgagacgtgg cctttcagtt tgcgcgtgac
    19021 ccaagtaaat gcgccgcgcg gcacgggcgt ggcaatggtc ggaatacgtg cttcatgcca
    19081 gccaatgccg gtgttgataa tggtcgcgcc cgccgcttca atggcctgcg ccagttctac
    19141 cgtttcggca aaagtgccgc cgccttcgac caggtcgagc atcgacaacc ggtagataat
    19201 aatgaagtcg ttgccgacgc gttcgcgcac cgtacgcact acttctacgg caaatcgcat
    19261 ccggttgcgg taatcgccgc cccactggtc actacgctga ttggtgcgca gcgtcagaaa
    19321 ttcgttgatc aaataccctt cggaccccat tacctctaca ccgtcgtatc ctgcctcccg
    19381 tgccagttgc gcgcagtggg cgaaatcgtc gatcagttgc aggatctctt catgcgtgag
    19441 ttcgtgagga acgaaacggt tgatgggggc ctgcaatgcg gagggagcga ccagatgcgg
    19501 ttggtagctg tagcgcccgg tatgcaaaat ttgcagggct attttgccgc cttcctgatg
    19561 taccgcttcg gtaatggtgc gatggtgtgg gatctggctg gcgtcgttga gcattgcgcc
    19621 accttccatg ccaacgcctg ttaaatctgg tgcaatgccg ccgctgacaa tcagcgccac
    19681 gccgtgacgg gcgcgttcgg cataaaacgc tgccagccgc tcggcaccgg ccgggtattc
    19741 ctccagcccg gtgtgcattg agcccatcaa cacgcggttt tttaacgtgg taaaacctaa
    19801 atccagcggg gcgaacagcg acgggtagct cataaaattg tccagtatgt aaaataattg
    19861 ttatgtggtc ggatgagttc taatttagcc gtcgccggag aaaagggaaa aggggagtgc
    19921 ggggtttgtg atgggattca aaaagaatac gcccggtggt aacgccggat gcgacgcttg
    19981 ccgcgtttta tccggcctgg gggattgcac atgtagggcg gataaggcgt tcacgccgca
    20041 tccgccagtg gcacggtgca gatgccggat gcgacgcttg tcgcgtttta tccggccagg
    20101 aggattgcac atgtagggcg gataaggcgt ttacgccgca tccgccagca gttgcaaatt
    20161 attgcttacg gaaaaaatcg ttatacaaca tatacaaatg cgcggcactc caggagaaat
    20221 ttggtgcgcc ttgctgtgcg ccagtcagcg ggttgtaatt ctcctgaatt gggccatcgg
    20281 cggttaatcc tttggcgtga cggaagaacg tatccgccag tttcagggca tcatcgcgat
    20341 aaccgtaacg ctccatccct ttcagaccaa accagaactg atccacccat acgcgcccac
    20401 gccagtagat atcagcgcca aaagccggat tggttaacgc cgccgttccc agcgggacaa
    20461 aggtgttgaa ctctttagga tcgagcatca ccttcaccac cgcgtcggca ttggcctgcg
    20521 ttgccgcacc gttaaacagc ggcgaccagc cttccggccc tttaccgcgc tcaacaatcg
    20581 gtttgcccgc gcagccgttc gccagtggtt tatcttcaat acgtacgtca tagtagaact
    20641 gggtagtcgg gtcgaacata caggtgttga tgtagtccgc gagctgctgt gccaactggc
    20701 gatagcgttt ggcctcttct ggcttaccga ggatggttgc catctccgcc agataatggt
    20761 tatcgctgta catatagctg gcctgatcca ccgactcctg caatagcgag tagcccagca
    20821 acgttccgtc ctgactgcgg ttttcggcga atttcaccgt ccagtcgcta cgtttgccgc
    20881 cattagcgac atatttatcc aactgttctt tgtcgataaa accaaagacg gcggcatcat
    20941 cacgccccga ttcccacgac gcagcgacct gtgccggaat ttccagactg tcgtactggc
    21001 ctttctccac cacgcgggcg tagttgttca gcccagactg cgtctcttct ttgttgcctt
    21061 tcttcaccgt aaacagcatc tcgccgctct cagtgttgtg ggctttgtcg cgggtcgcgc
    21121 catattccgg cacgccgttg ccgttgtgat cgcggttacg taaccaccag tcgtgatagg
    21181 ccaccagttt cgggtacatc tctgccaacc aggctttatc ctgggtgacg ttgtacactt
    21241 ccatcaccga ccaggcggca aggctgggtt tggtattacg ttcgttccag ttgccgccat
    21301 cgccaccacg ctcggggcta aggttccacg ctatcaggtc ggggacaaag cccacatcct
    21361 gcggacgcac gctatcgcca ggctggatct gccaggagaa gaccgcgcgg atattctctt
    21421 tggcgatgtc cggattgaaa tgcgccatcg caaacgcctg cttccaggta tcccacggcc
    21481 aggtctgatt gccggagaac cagcgcccgg tcaccgacgg tgtaacggtg ttaaatttca
    21541 ccgcaccgcc cggtgagcgc cagttaccgt tgagcgtttc gatcgctttc accgcgacgc
    21601 gcgtctgttc cggcgtcgca tccggattgg ttaacccttt cttcaggtat tcttcccagc
    21661 gttgctgcga ggcggtgaga taaaacgccg gacgcgccag aatatcgcgg atctgcattt
    21721 gctctttgct aacttcctga gcggtcagca gatgggaata ggtggtgtag agcgtggtcg
    21781 aaccgttgat atgcgcctta ctggtaaagc gattaccgtt gatttcagtc tgcaccggca
    21841 gggatttatg cacctgatat tccgattcgc cggaggtcag cagatcccag gtggcgcgca
    21901 ctttgccaaa ggtgactttc aggccatcac gggtggcgct gattttgcgc tgatagtcag
    21961 ggtattcgtc agcaatggtt ttatcggaaa gcggtttccc ttctttcgct tccagttttt
    22021 ccagcagttc gccgtcccac accagatcca gcggtttatt gctggtgatt ttggtttcca
    22081 gtagtgacgt gcgcggcgtg gcgaagcgca gagtcatttc gacttgcaca tcttttgctg
    22141 tcagtttttg caccagcgca ccgggaatac tgtatgcctc cagcgtaaag tcgactttct
    22201 tgccgtcctg ccagacggtc aggcggtcga aattgctggc cataaagttg atgtactctt
    22261 ccgtcagcag cgcaacgccc ggaaagccgc ccatggtgtt agggccgtct ggcaacagat
    22321 gaccatgcca ggctccgaga tcgaaaaacg gattaaaacg ctggtgatcg tcgtaatcgt
    22381 aatccttcat gtactgcggc gcgccagtac ggttaatcac gtttttataa ttgtcggcgt
    22441 tagcggcatg ggcggaaaaa cttatcagca gagcgcaggt tactggcgtt aaaatagttt
    22501 ttattttcat gaaattacag tccttattac cagtagaaat attgcatcag gaagatttgg
    22561 tcgctgggat cgtgagaggc atttttcatg tagaagcggc tatcaaaagc cgtggcgaaa
    22621 cgtccgccgc cgtattcgta ccagatacca aactgcatga aagaggtggt ttcatcgtca
    22681 gcgtggtcag ggcgatgttt ggcgtaggtg taagcggtgg agagataaac gccctttaac
    22741 gcttcataca tggcgctcac catcatccca ctttcacttc ccggtacgtc cagcccgtcg
    22801 gcgcgtccgg tatggtgcca ggcgcgagcg gcaaagttag gagttagcag gcccgtgaga
    22861 tagagctgtc ctgtgccgtc ggtgatttcc agaccgttca tccaggtgat gccttcggca
    22921 aggtcatact ggatgtaacc gttcatcatt gccggatagg tgtatttgtc gtcatagcga
    22981 tcgtacttgc caaaatgtag ccaggctttg ctttcgtcat gatgtccggc aggtgtggcg
    23041 gtgacgctat aacgaaagtt gccgcttaag ttctgcacct tcagcgcggt cataatgtcg
    23101 cgggtattgg gaatgacgta gcccagatcg ggggtgaagt cgccccatca ttgcaggtca
    23161 tccagcgagg aatcctgacg cagactgagc atgatttcgg tgccatcatt ggtacgataa
    23221 ccgccataga aacggttaat tccgccctcg aaaccacccc agctatggtc tggcacccag
    23281 gcattgccgt cgtggtcggc ctgcacggtc cagccttcgc cgtaaatcag gccgaaccag
    23341 gagccgtact tcatctccag gccaccttca atataggtac cgtcgctgta tttatgttta
    23401 ttctcaccct ctaaatccat atatccacca acccctaatt cgccgtaaaa acgaaatgaa
    23461 ggagaagatg tcgaggtaat tgcaggtgtt tttgtgtcgt catgttctgc cggacgtgct
    23521 tcttctcctg ccgcagaaaa ggcgtagcaa aacggcagag caagtaatgc tctgcatggt
    23581 gcagtgatta atttcattgt gttaatccat gagctaaaga aaaagtacag ccttttatcg
    23641 ttgtaattat aaaaatataa aggcagaaac tattttcccc ctccccatcc aggaagaggg
    23701 aaaatgcctg ctcatttttt attagtctgc tcaatcaaca ttgctggctg gcgtcgcttc
    23761 aatatggcgc tcttcagccg ttaatccctt tatatattta ctgtatttcc accacgcaaa
    23821 gccgaggaag ataacaatac cgccgacgtt ataaaaaatg atggtcagaa tattcgcgcc
    23881 agtcgggaat gtcgaagcga caaaccctac ggcaaatatc gcaatcagca ttgaaacaac
    23941 gataataccg gtgcggcggg agcccatgcg gaaatcgcgt ggcaggtgat ctaatttggc
    24001 gcgtaaattc aggtaagcca gcatgatgaa taacggcgga agcatggacg ctgcggcagt
    24061 catattaata atagtattca tcagatcctg cacggtattg gaaccgagca tcgggataat
    24121 catcagcggg atgacgatca ggaactggat ccacgctgcg cgcgctggaa cgccgttttc
    24181 gttcagttcg acggttttct taccaaagat gccttccggg atttcggaga agaaaatttt
    24241 caccggcgtt gcggtccaca tcagcaggga accgaacatc gcggtaaagg acaccagacc
    24301 gacaaagcga ttcatcaacg cttccggtag accaaaatac gccgccatgc cgtggaatac
    24361 ctgcaccgat ccgccggtaa atttcaactc tttgctgctg acgaagacgt taatcagcac
    24421 cgaggagacg gaatacagta cgccgataaa aatcccggcg aggatgatca ctttaacgaa
    24481 cgatttcgaa ccgcctttga cgtcgttaac gtacacagcg acggactccg cgccacctgc
    24541 ggccataaag atccaggtgg taacgccgag gaacgcccag ttgaagttcg ggatcatcgc
    24601 gtcaacggtg atggggtcag caggctgtac gccgccaacc agcgccgtac ctgccagtaa
    24661 aatgtaggag agcgttaaca gcagcatcag cgttgaagtg acggaggtaa ttggccccaa
    24721 cattttcgcc ccgttggtgg aaacccaggt ggagaaggcg aacagcacca tactgataat
    24781 ggtggtggca accggcgtca taatatattc gtagccgagg aaggcatacg aagcataagc
    24841 aataacgcgc ggcaacagtg aggtgaaaaa gaacaggttt acgaaccagt aggtataggc
    24901 agtaataaat gcccaacgtc cgcccagcga actttttacc cacgcgtaga caccggcttc
    24961 tgagttttta tttaacgaaa caaattctgc gatgatcaga cagaagggaa taaaataaaa
    25021 aatcgtcgcg aggaaaaaca tcggtgccga ggccagtcca agctcaatat tattattgat
    25081 aacgttgtta aagctgtaaa cggcggcaaa agtcagcgag agcaagccaa atttgccgat
    25141 tgtattacgt ttggtatcag acataacaca tctccgaaaa tgtgtagggt agcggtggcg
    25201 gcaaactcct tttgccgccg tagttgttat ttgttatgga aataaccatc ttcgatggtg
    25261 actttgagaa ccactttttt gaccgcgtta ttgcagataa accgatacgc ttcatggata
    25321 tcgcaaataa cgatttgccc ttcgtggacc tcaacggttt ctccgcagcc ttttaaatat
    25381 tcacggtcag tttcatcacg ataatattcc actacctgta atgtctcttt cggcgcatat
    25441 tcaatttttt gctgcccttg caggtagtaa tgcacttcaa aatagcgtcg atgcccggta
    25501 aatagcgcat cggtcgcgga gtctgtctcc acgcggtaag tcaatgagtc accgatggag
    25561 tgggcgacgc caggctgaat gttgtcgata ttttcaatcg cttcaacgca gcgttgccac
    25621 ttcttgccag aggcgtaaat ctggcggaac tgttctaagt tatcgatgat cctcattgct
    25681 tattctcgct gtgcaaattc gtggaaaaga accctgcgcc gaactcatac gacgccaggc
    25741 tttgcgcggt agcttctccg ccagaaaccg gcagcaacgt aaagccgtag ctgaagtcac
    25801 ggaaccagac gcgccaggag tccagcacct cgctgcccca ggagttggag ccgaggccaa
    25861 gcagctggtg gtcgaggttc agggtgatgt catcactgcg ctgcagctcg ttacagtgct
    25921 gggcagcgtg gatgttttcc tgggtatagc gccaggcgct gaagttaatt gggcgctgcg
    25981 gaaccaccag cagaccgttg ccgtggcggt tagtcagtgc cgtccagcgg acatgctgac
    26041 ggttgccgtt gttctgcggg aagggatagt tctcgaacat ggcatcgacg gtgctgcgcc
    26101 agatatcgat gatgttagcc tgctggctgt cggcgtagtt ttcgcccggt ccacgaccgt
    26161 aatacgccac ctgatcgtat tcgccgttaa tccccatggt gaaaccgatg cacggaatga
    26221 tgtgcggata gtcgccgtaa cgctcgccgg aaagcgccac gttaacctgg ccatcggcag
    26281 cgatgcgcca gatgtaggtg cagcgcatcc cgaagtcaaa caccggcggg gcaataactg
    26341 tgcggctgat gatcaacact tcaccatcgc tctgttctac ggcaaagtcg cgcagatgtt
    26401 cctgcatgat ctgcaaatga ttcggttgcc acagcccttc gtactcctgc ttgtggttgt
    26461 cgatcattgg cttgaagaag ttgatctttg gctcgcgggt cagcagcgac tcgccattca
    26521 cctgccagga tgtcggtttg ccactcattt ttgagaaggt gatcgcgaag ttgtagccgc
    26581 gaacggtgca gctcaaacga tcgtcttcca gcgtcagcgg acgcgcatta tttggtgcga
    26641 aaggcactgg ttgcgcggtg ttttccttca gcgggaactg ataagtggcg atagaatgcc
    26701 cggcttcgct gtagcgggtg cgggaatctt tggtcaccgt aatgttgagg aacgcttcgc
    26761 gggcgtccag ctgcggcagc gtgatctgca agggggcttc gctgttcggc gcaacgtcgc
    26821 gcagtttaat ctgctgcgtc gcgagcgttt caccttcgac gcgcacctct gcgtgcaggg
    26881 tgtagtcatc aagcgtggta aaccacagtt tattttcgac tttcagctcg ccgcgagtca
    26941 gatccagcgc gtggattttt accggcgcga taacctgttt gtactctttc aggcccggtc
    27001 ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg ttgggatagt
    27061 cgccgtagtc gccgccgaat ttataccaga cgttgccgtt gtcatcctgc gcctggatcc
    27121 cgtggtcgca ccattcccag acataatgtc cctgaatgca atcgtgctta tagaagacgt
    27181 tctggtactc cgtcagcccg cctggtccgt tccccatcgc atgagcatat tcacagatga
    27241 tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg cgggtgtaca
    27301 tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatgc accagtcgcg
    27361 tgtcatccag cgccttcgct gcgtggtaca tcgcgcggat gttacagcca tagccggatt
    27421 cattgcccag cgaccagatg atgatcgacg gatggttttt ctgcgcgtga atatggcgaa
    27481 caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg ctgatatcgc
    27541 cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac aagccgtaga
    27601 tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc acggagttga
    27661 tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca acggcgcggc
    27721 ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa cggttattga
    27781 tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt ggcaccactt
    27841 ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga taaggggatt
    27901 ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg gtcagttttt
    27961 caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca aacagggtat
    28021 attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact tcgcaggaaa
    28081 gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg ttaatatgcg
    28141 ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgctgaccac cacatatcct
    28201 ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac aggttgtcgc
    28261 cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc ttgctgaaac
    28321 ccacatactg accgttaaca tagacttcaa aataggtttc gacgccgtca aatttaatca
    28381 gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag gcaccggttg
    28441 ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg tcggtatatt
    28501 gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc ccccagtcag
    28561 ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa aagtggaaat
    28621 tccactgacc gcttaagggc agaaacaggc tgctggtttc gcgggcaaag gtacgcgctt
    28681 gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt tcgtgggtga
    28741 gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg gaaccattca
    28801 tggtgatggt agtttgtgta attgctgggg agtttagtaa aatttttact aaaaagtagc
    28861 aggtaaatgc atttacttta actgggtcac gaaaataaac ggcagcattg tccggcaaaa
    28921 acgattgccg gacagtgtgt aaggggattt aacgggtcgt gccgcgcagt tttaatttgc
    28981 tgggaacgaa gactaacagc ggcagcgcgc gaccatcgcg ggctttttca tacaccaggt
    29041 taacgccctg acttcccatc atctcggaat ggatgcgcac ggtggagagc ggcggaaagg
    29101 taaatcgcgc ggtggggata tcgttaacgc tgataagcga aatatcctgt gggatgttca
    29161 ggcctcgttc atgaattgcc cgcagtacgc cgatagcaat ggaatcggaa gcaacaaaca
    29221 gtgccttcgg atagtcttcc cgcgccagca tttgttttgc cagttcataa cccgacgaac
    29281 tggaaaaacc gccgcgccag atatcctctt cgcgtaccac ttgtttcagt cggccatatt
    29341 ccgcaaaggc gacctcacga atatccgcct tgccaggctc atcttcaccg ccaataaaac
    29401 caatacgatt aacgccctgg ttgatataga agtcgatgat ttctttactg atgcgtgcca
    29461 gatcgatatc caccgcatcg taaccgctgc cgggttcgtg aaagtcgata aaacagatat
    29521 tgtcggtcaa cgcacaggcg gcggcgcgca gggcgggcgt gggtttgccg acaattaaaa
    29581 taccggtgac gtttttaatg tctggtaatc cgctgtgttc ataacagttg gtgagctcaa
    29641 tgcccagctt ttcgcactga gtttcaatgc cgtggcggat cgccagatag taaggatcgt
    29701 tgatctccag ctcctgttgg tagctgtaga tagccagaat atggtgttgg ttgactgcac
    29761 ctgtctggag tttacgggca ctactggtct tgtactccag cttttcggcg atctcgagaa
    29821 tgcgatgttt cgtctcttct ttcacattca atgtcggatc gtcatttaag accctggata
    29881 ctgtcgccag ggatacgcca gcttcgattg cgatgtcttt tagtgttgcc atgtttacct
    29941 tttttacttt ccctgatgtg cgcccccatt gtagaggaag tgcttatcgg gtcacctgtt
    30001 ttcagtaaat aaagcgcgtt tctttgacgc tgtaacggta acacagcggc tcacgaatgt
    30061 gatcacgcct gcattctcca tttttagcct acaggattga gaaaaacttt tgctatagtt
    30121 tagtaaaatt ttacccgaag ggaggaaaca atggaaaccg tggcttacgc tgattttgca
    30181 cgtctggaaa tgcgcgtcgg aaagattgtg gaagtgaaac gccatgaaaa cgccgacaag
    30241 ctgtacatcg tacaggttga tgtgggggaa aaaacactgc aaaccgtgac cagcctggtg
    30301 ccgtactaca gcgaagaaga gctgatgggg aaaacggtgg tggtcttatg caatctgcaa
    30361 aaagcgaaaa tgcgtggtga aacgtcggaa tgcatgctgt tgtgtgcgga aaccgatgac
    30421 ggcagtgaaa gcgtgttgtt aacgccggag cggatgatgc ctgcgggcgt acgcgtcgta
    30481 taaaaagatc ggatggcgac gtcgtatcgc catccgattt gatattacgc ttcttcgaca
    30541 cttactcgca tggccgccag cgccttacgc gccgctttga tcaccagttc acactgttca
    30601 atggtcagtg tcagtggcgg ttcaatgcgg atcgttttgg cgttattgag cgttccggcc
    30661 accagtacgc gctggcggaa catctcgctg gcaaagttat agccgatttc gttatcaaca
    30721 aactcaattg ccatcaacat ccctttacca cgcgcttcct gtaccagatc gggatattcc
    30781 cgcgccagtt gacggaaacc gtccagcaac atatcgcctt tttgctcagc ctgagccggt
    30841 aagttctgct ccagcaacac attgatggtc gccagcgccg ccgcacaggc cagcgggttg
    30901 ccgccaaagg tggtggtatg caggaatggg ttgtcgaaca gaactgaaaa cacctcttca
    30961 gtggcgatgg tcgcgccaat cggcatcacg ccgccgccga gcgctttggc aaggcagagg
    31021 atatccggct gtacgttctc atgctcgcag gcgaacatct tgcccgtgcg ccccatgccc
    31081 gtttgtactt catcgaggat catcagtgcg ccgaactcat cgcatagctt acgtacggcg
    31141 gtgagatagc ccggcggcgg cagaattacg ccaccttcac cctgaatcgg ttcgaggatc
    31201 accgcagcca catcatcacc ggtttttttg cactcgttaa gagccgtgcg catggcttcg
    31261 atattgccaa acggcacatg acggaagccc ggcagcagtg gcataaacgg tttgcggaag
    31321 gtcgatttcg ccgtggccga cagcgcgcca agtgatttac cgtggaacgc gccgctggtg
    31381 gcaataaaag taaacttgcc gcgcggtgac tggtaagctt tcgccagctt cagcgctgct
    31441 tcaacggact cggtgccgct attacagaag aagctgtatt tcagtttacc gggcgttagc
    31501 gcagcaaggg ttttcgccaa catcgcccgt aacggatcga gcagctcctg gctgtgcagc
    31561 ggttgtttcg caagttgatt ctgtacggcg gaaaccacaa ctggattacg gtgccccacg
    31621 ttgaaaattc caaaacctcc caggcagtcg ataaactcct gtccctgggt gtcgacaagc
    31681 gtatttaaac ctcccgcttg ccactctacg gctccgtaat ccccgccggc ggtaacagat
    31741 ttgcgatact ctaaaaaccc cggattgaca tgctctttga agtattcaat cacctctcgg
    31801 ttaagtgctt tcatctcctc atgatccagc gttcgcttct caatgagatt cagggcgtgg
    31861 gcgctgcacg ctaaagccga tgcgctcgaa ggtaacctgt tcaaaatatg ctccggaggc
    31921 tcgcgtatca catgataccg atttaagtat tgcagggatt gcgccactcc ggctgcgatc
    31981 gcgaaaatcg ggataaacac aaattcggtt atcatttgtg caatatttaa tgctatctgt
    32041 taacatttgt tgttattttg cacggttttt gtcgggtatg gatttgatga ttgctgcgca
    32101 aatgcacgtt ttcagggcaa taattgcctg atttttgggc aagataatta acgttttcgt
    32161 atagtaacag agtgaaagta ttttaaatca aagaattaga tttgtgcttt aatcgcaaat
    32221 tgcgatctaa atcaaattaa tcggttaaag ataaccgcag cggggccgac ataaactctg
    32281 acaagaagtt aacaaccata taacctgcac aggacgcgaa catgtcttct catccgtatg
    32341 tcacccagca aaataccccg ctggcggacg ataccactct gatgtccact accgatctgc
    32401 aaagctatat cactcatgct aatgacactt ttgtgcaggt gagcggcttt accttgcaag
    32461 agttacaagg gcagccgcac aacatggtgc gtcacccgga tatgccaaaa gcggcgtttg
    32521 cggatatgtg gttcaccctg aaaaaagggg agccctggag cggcatcgtg aaaaatcgcc
    32581 gcaaaaatgg tgaccattat tgggtgcggg ccaatgcggt accgatggtg cgcgagggaa
    32641 aaatcagtgg ctatatgtcg attcgtaccc gggcgacgga tgaagagatt gcggcggtgg
    32701 agccgctgta caaagcgctg aacgccggac gtaccagtaa gcgtattcat aaaggcctgg
    32761 tggtgcgtaa aggctggctg ggtaaactgc cttcattatc gcttcgctgg cgggcgcgtg
    32821 gagtgatgac cctgatgttt atcttgctgg cggccatgct ttggtttgtt gctgccccgg
    32881 tggtgacgta tttcctctgt gtgttagtgg tattgttggc aagcgcttgt tttgaatggc
    32941 agattgtgcg cccgatagaa aatgtcgccc gtcaggcact gaaggtggcg accggagagc
    33001 gtaatagtgt tgagcacctg aatcgcagcg atgagctggg gctgacatta cgcgcggtag
    33061 ggcagcttgg cctgatgtgc cgttggctaa ttaacgatgt ctcaagccag gtgtccagtg
    33121 tcagaaatgg cagtgagacg ctggcgaaag gcaccgatga actgaacgaa catacccagc
    33181 agacagttga taacgttcag caaacggtgg cgaccatgaa ccaaatggcg gcgtcggtga
    33241 aacagaactc tgccacggcg tcggctgccg ataaactttc tatcaccgcc agtaatgcgg
    33301 cagtgcaggg tggggaggcg atgaccacgg tgatcaagac aatggacgat atcgccgaca
    33361 gtacccagcg cattggcacc attacttcgc tgattaacga tattgcgttt cagaccaata
    33421 ttctggccct gaatgcggcg gtggaagcgg cgcgtgccgg cgaacagggc aaaggttttg
    33481 cagtggtggc aggggaagtg cgtcatttag ccagccgcag cgctaatgct gccaacgata
    33541 ttcgcaagct gattgatgcc agtgctgata aggtgcaatc cggttcgcag caggtacacg
    33601 ccgccggacg gacgatggaa gatattgtgg cacaggtgaa aaacgtcacc cagttgatcg
    33661 cccagattag ccattcaacg ctggaacagg ccgatgggct ttccagcctg acccgtgcag
    33721 tggatgagct taacctgatc acccagaaaa atgccgagct ggtggaagag agtgcgcagg
    33781 tgtcggcgat ggtgaaacac cgcgccagcc gactggaaga cgcggtgacg gtgctgcatt
    33841 aatcggtatg ccggatccgg cggttggagc acaatgcctg atgcgatgct ggcgcatctt
    33901 atcaggcctg cggggtgtgt agcgggtgta ggccggataa ggcgcgtcaa gcgtcgcatc
    33961 cggcaattgc accgcgccac tggcggatgc ggcgattaat ccagttgcgt aatatcaaaa
    34021 gcggcgcggt cgatcaccgc aatgatcttt tttatttgtg catcactgat atcgctctgg
    34081 ttgacgtgta aatccagcac cgctttaaaa ttatccagcg cccgcttcat ttgcgggttc
    34141 tggcgcagcg ccgcgccaac gcaacgcgct ttgatgcgtt cttcaatcat ctccacctgt
    34201 tcgcggtttt cttccagcca ctgcgcgcct tgttcggtca gcgcaatctg cttcttacca
    34261 ccttcctctt cgcggatggt aatcagcgac tgctcctgca gaaaatccag cgttgggtag
    34321 atgacgcccg ggcttggggt gtaattcccc tgggttaggt tctcaatcgc tttaatcaat
    34381 tcgtaaccgt ggctgtcatc gcgcgagaga atatccagaa tcaccagacg caattcaccg
    34441 tgaccaaaga aacgttgccg acgaccgccg ccgcgaccgt ggcgaccacc gcagcattga
    34501 ccgtgttcat gctggtgacc gtgtccgcag tgctcgtgtt ctgacttctc acctttgcag
    34561 cagccctcat ggcgtggctg gccttcatgt ttacaacacc cttcgtgatg atggctcata
    34621 tcagcctcct tttgcttgat tagatatatc taatttatat ctgatgtgag cattactgca
    34681 agtacaaaaa tgatgtttaa acgtggaagc acctcacatc caggccggat aagacgttca
    34741 cgccgcatcc ggcagcattg ctatggcact gaatgccttc acatacttca gatatatcag
    34801 ttctacaaat cgcttgcatt tatcatgatt aacaatcatt atcatttacg aaaattattt
    34861 agatatatct gattactatc acgaaggcga taacaatgaa taactccccc cgctacccgc
    34921 agcgcgttcg caatgatctg cgcttccgtg aactgactgt gttacgcgtt gagcgcatca
    34981 gcgccggttt tcagcgcatt gtcctcggcg gcgaggcgct ggacggtttt acatcgcgcg
    35041 gctttgacga tcacagcaaa ctcttctttc ctcaacctga cgctcacttt gtgccgccaa
    35101 cggtaacgga agagggcatc gtctggccgg aaggaccacg cccaccgtcg cgtgactata
    35161 cgccgctgta tgacgaacta cgccatgaac tggcgatcga tttctttatt cacgacggtg
    35221 gggtcgccag cggctgggcg atgcaggcgc aaccgggcga taaacttacg gtggcaggtc
    35281 cgcgcggttc gctggtggtg ccggaagatt acgcgtatca gctgtatgtc tgcgatgaat
    35341 ccggaatgcc cgcactgcgc cgccgcctgg aaacgttgag caaacttgcc gttaaaccgc
    35401 aagttagtgc gctggttagc gtgcgggata acgcctgtca ggattatctc gcgcaccttg
    35461 atggttttaa tatcgaatgg ctggcacatg atgagcaggc ggtagatgcg cgtctggcgc
    35521 agatgcaaat ccctgccgat gattacttca tctggataac cggcgaaggt aaagtcgtta
    35581 agaatttaag ccgccgcttt gaagcggaac agtatgaccc acagcgggta cgtgcagcgg
    35641 cttactggca cgcaaagtaa ctgatatata aaaagaaaaa ggctgacgat ttctcgtcag
    35701 cctttgctat atctggtggc ccctgctgga cttgaaccag cgaccaagcg attatgagtc
    35761 gcctgctcta accactgagc taaggggcca tggtagcgga ttataaagta actccgtgtc
    35821 gcaatccagc cattaccgtg cgcctgctgt ttttataaac aatgtatttt caatccgtta
    35881 tacttttcct ggtgatgtta gaaaggagta aatatggttg aggatatttt ggctccaggg
    35941 ttacgggtcg tgttttgcgg tatcaaccct gggctttcat ccgctgggac tggttttccc
    36001 tttgctcatc cagcaaatcg cttctggaag gtgatatatc aggccgggtt taccgaccgt
    36061 cagttgaagc cgcaggaggc acagcatctg ctggattatc gttgtggcgt caccaaactg
    36121 gtagaccgtc caacggtgca agccaatgaa gtttcaaagc aggagctgca cgcaggtggg
    36181 cgtaagctga ttgaaaaaat tgaagattat cagccgcagg cgttggcgat tctgggcaaa
    36241 caagcatatg aacagggatt cagccagcgc ggtgcacagt gggggaaaca aacgctcacc
    36301 attggttcga cgcagatttg ggtgctgcca aatcccagcg gtttaagtcg cgtttcacta
    36361 gagaaactgg ttgaagcgta tcgcgagctg gaccaggcgc tggtagtgcg tgggcgataa
    36421 aaaacgccac cgaaaaggtg gcgtttgtgc aaagggacta gtgccgggtg cggcgtaacg
    36481 cctgatccgg cctaccgatt aatcgtccag gaagctacgc aacacttcag aacggctcgg
    36541 gtgacgcagt ttgcgcagcg ccttcgcttc gatctgacgg atacgttcgc gggtaacgtc
    36601 gaactgttta cccacttctt ccagcgtgtg gtcggtgttc atatcgatac cgaaacgcat
    36661 acgcagaact ttcgcttcac gcgcggtcag gccagccagc acgtcgtgcg ttgccgcacg
    36721 caggctttcg gtggtcgcag aatccagcgg cagctcgagg gtggtatcct cgatgaaatc
    36781 ccccagatgc gaatcttcat catcaccgat cggcgtttcc atggagattg gctctttggc
    36841 gatcttcagc actttgcgga tcttgtcttc cggcatcagc atacgttcag ccagttcttc
    36901 cggcgtcggt tcacggccca tctcttgcag catctggcga gaaatacggt tgagtttgtt
    36961 gatagtctca atcatatgta ccggaatacg gatggtgcgc gcctgatccg cgatagaacg
    37021 ggtgatcgcc tgacggatcc accaggttgc gtaggtggag aacttgtaac cacggcggta
    37081 ttcgaattta tcaaccgctt tcatcagacc gatgttgcct tcctgaatca ggtcaaggaa
    37141 ctgcaagcca cggttggtgt atttcttggc gatagaaata accagacgta agttcgcttc
    37201 aaccatctct ttcttcgcac ggcgggcttt cgcttcaccg atggacatac gacggttgat
    37261 atctttaacc tgctcgatgg tcaggccggt ttcttcttca atctgctgca gtttctgcag
    37321 ggcgcgatgc acttcttcag agacatcgtg cagtttttcc gaccacggct tgttcatcgc
    37381 aattgccgcg ttgaaccagg tatcgctggt ttcgttgccg gtaaacaggg taatgaagtt
    37441 tttcttcggc attttgcact gctcaacgca gagcttcatg atcagacgtt cttgcgtacg
    37501 aacgcggtcc atcatgacgc gcatgctgtt gaccaggtag tcaaactgct tcggcaccag
    37561 gcggaactgt ttgaatactt cagacagttt caggatctct tcctgagcgg cagcgtgact
    37621 gcgacctttc gctttgatgg tgtcacgcgt tacaacgtac tgagcgcgta gttccgcaaa
    37681 tttttcgcga gccagttccg ggtcgatgct gttgtcatca tcggcgctgt cgtcatcgcc
    37741 atcttcttcg tcttcatctt cgtcatcgtc cagatcttcc tgggaaagct cagaaccgac
    37801 gtgagtggcg gtaggtgcca gatcttcttc tgcgttcggg tcaacaaagc cggtgatcag
    37861 atcggacaga cgcgcttctt ctgcttcaac acgatcgtac tgttccagca gataggtgat
    37921 cgcttccgga tattcagcaa cggagcattg aacctggttg atcccgtctt caatacgctt
    37981 agcgatgtca atttcgcctt cgcgggtcaa cagttcaacg gtgcccattt cacgcatgta
    38041 catgcgtacc gggtcagtcg tgcgcccgat ttcagattcc acgctggaaa gcacctgcgc
    38101 ggcggcttcg gcagcatctt cgtccgcggt gttttcagcc agcatcagat catcggcatc
    38161 cggtgcttct tccatcacct gaatgcccat gtcgttgatc atttggatga tgtcttcgat
    38221 ctgatctgaa tcgacgatat cttccggcag atggtcattg acctcggcat aggtcagata
    38281 gccttgctcc ttaccacggg tgacaagaag tttcagctgt gactgcgggt tttgctccat
    38341 aagacggtat ccacacttat ttcatgaggt tggtgttggg cgattgaccc gatgtccttc
    38401 agccgttaac gctgaaatcc aacggttgtt taccgccaac gataattacg agggcgtact
    38461 tatatatttg ccgctgcctc tcagtgcggc tgtcgggggc ttcccgatcg ctcttcggca
    38521 cttaagccgt taaatcactt tttcgccagc gcctggttta atgtccagag ctccaggcgt
    38581 tcttcgttgc ttaaaccatg cgtgcgctca cgagcgatta actcttcctg gcgcagttca
    38641 agcagcgaat caaacatatg gttgagtgag tcggtgaagg tttgctcagc aatattctta
    38701 tctgctatat cgtcccacat cgacagtttt tcaagggtgg cagcattatt tgtaccacga
    38761 tagtgctcta aaagttgccc ggtggtcaga cctggctggg agagacaagt gttgaccagt
    38821 tctctgaata agccaagtcc agggagctta ttttcatcca gattctcaag cggcgggacc
    38881 aacgtcgcta attctggatt ttgcaccagc aaccctataa gtatacgcat ggtcgtgcgt
    38941 tttagctgcg gaacagggcg agaaacgccg ctctctgccg cttttggcat taatcgttca
    39001 agctggctgt catcaagtat gcctaatttg ttgcctaatt cctgacgaag atatattcgc
    39061 agcgtttcgc ccggcacttg cgatatcaat ggcagtgcca gcgtactcaa acgtgcgcgc
    39121 ccgtcagggg tactcagatc aacttgcggc atcaggctgt taaacagaaa tgcggaaagc
    39181 ggcatcgcct gctccatccg cgcttcaaac gcttctttac cttcttttcg taccagcgtg
    39241 tcagggtctt cgccatccgg caaaaacata aagcgtagct gacggccgtc tgtcatgtaa
    39301 ggcagcgccg tttccagcgc tcgccatgcg gcatcgcggc ctgcacggtc gccgtcataa
    39361 cagcaaatga cattgttggt cgcgcggaac aacagttgta tgtgatcggc ggtggttgac
    39421 gtacctaacg acgcaacggc gtaattaatg ccgtattgcg ccagcgccac cacgtccata
    39481 tagccttcga ccacaagcag acgattgggt tcagcgttat cctgctgcgc ttcataaaga
    39541 ccgtaaagct ggcggccttt atggaaaatg tctgtttccg gcgagttcag gtatttgggg
    39601 gtatcgttgc ccagcacgcg cccgccaaaa ccaatcaccc gaccgcgttt atcgcgaatg
    39661 gggaacatca cccgctcgcg gaaacgatcg taactgcgtc cctgatcgtt agtgaccaac
    39721 atccccgcat caatcaatga ctggcgattt tctggattgc cgccaaaccg cttcaggacg
    39781 ttgtcccagc cggggggcgc aaaaccaatc gcaaagcgag cgataacctc gtggcttaat
    39841 ccgcgttttt ccagatactg gcgcgcagac gtggcaacag gttgttgtaa agattgttgg
    39901 taaaacgtat tcagaccgtc catcaactga taaagcgttt gcctctgatg gcgctcgatc
    39961 tggctggggc cgctgcctgc ttcaaatggc acttcaagat tgtgcattgc tgccagctct
    40021 tcgaccgttt caacgaactc gagcttgtcg tagttcatca ggaagtcgat cgcgttgccg
    40081 tgcgcgccac atccaaagca gtggtaaaac tgtttctcac cgttaacggt gaatgacggg
    40141 gttttctcgt tgtggaatgg acaacacgcg tggaaattct tgccctgctt tttcagcttc
    40201 acacgggcat cgatcagatc gacgatgtca gtgcgtgcca gcagatcatt aatgaatacg
    40261 cgtgggattc gtccagccat aggccccgat ttttagcaat tcataaacga aaataagccg
    40321 cgcattcctt tcggaagcac ggccttacaa ctacaactcg gtctgatcgg agagcaacgc
    40381 tctcggggaa ttagtacaga cgagtgcggc gtgcgttttc gcgagccagt ttcttcgcgt
    40441 gacgtttcac tgcagaagct ttagcgcgct tacgttcggt agtcggtttt tcatagaact
    40501 cacgacgacg aacttccgcc agaacacctg ctttttcgca ggaacgcttg aagcgacgca
    40561 gagctacgtc gaacggctcg ttttcacgta ctttaattac cggcatgtgc ctctcacctt
    40621 tgattaattc ggtttgccgc tggcatcaac gccagcttat ttcaaaatgg tgcggaattt
    40681 tactgcaatt gctgctgctt tgtaaagcac cgcggccttt tttgaaaggg acttttataa
    40741 gggtgaggag tatacacgag gctttctcca ggggcgaaca aagttttaca tcaacccgca
    40801 ttggtcctac actgcgcggt aataaagcga ggtaaaacaa gtcatgcgtg tactgggtat
    40861 tgaaacttcc tgcgatgaaa ccggcatcgc catttacgac gatgaaaaag gtttgttagc
    40921 caaccaattg tatagtcagg tgaaattgca cgctgactac ggcggcgtcg tgcctgaact
    40981 ggcctcccgc gatcacgtgc gcaaaaccgt accgttgatc caggcggcgc taaaggagtc
    41041 tggtttaacg gcaaaagaca ttgatgctgt ggcctatacc gcaggccctg gattagtcgg
    41101 cgcactgctg gttggcgcaa ccgtggggcg ttctctggcg tttgcctgga acgttccggc
    41161 aatcccggta caccatatgg aagggcatct gttagcgccg atgctggaag ataacccgcc
    41221 ggaatttccg tttgtcgcgc tgctggtgtc cggcggtcat acgcagttaa tcagcgtgac
    41281 tggcattggt cagtacgagc tgctcggcga gtctatcgat gatgccgccg gtgaagcgtt
    41341 tgataaaacc gcgaagctgc tggggctgga ttatcctggc ggaccgttac tgtcgaaaat
    41401 ggcggctcag ggtactgccg ggcgctttgt tttcccgcgt ccgatgaccg accgtccggg
    41461 gctggatttc agcttttctg gtctgaaaac ctttgcggcg aacacgattc gtgacaacgg
    41521 caccgacgac cagacgcgtg ctgatatcgc ccgcgccttt gaagatgcgg tggtcgatac
    41581 gttgatgatt aagtgtaagc gagcgttgga tcagacgggc tttaagcgac tggtcatggc
    41641 gggcggcgtg agtgctaacc gcacgttacg ggcgaagctg gcggaaatga tgaaaaaacg
    41701 ccgcggcgaa gtgttctacg cgcgtccgga attttgtact gataacggcg cgatgatcgc
    41761 ctatgccgga atggtgcggt ttaaagcagg cgcgacggcg gatctcggcg ttagcgtgcg
    41821 tccgcgctgg ccactggcag agttaccggc tgcgtaaaac tatccggtgc cggagagcaa
    41881 tttccggcac cgtcctcact taaagcaaca ccacgggcat ccacagcaag ccggtaatca
    41941 ccagcaacac gaggaatatc agtccaaaaa tcgcccccag tcgccagtaa tccaccgttg
    42001 gcagataacc actaccgtag taaatcggac tgggtccggt ggcgtatggt gtaagaatgc
    42061 tccccagacc aattgccgcg ccaaccatca ggcagaacac cggcagcggg atttccggca
    42121 tcgccagcgc agcggcaatc atcatcggtg cgagggcgga ggtatacgcc gtggcgctgg
    42181 cgaaaaagta gcgcagcaga taaaacacca caatcaacgc caccatcacc atcgttggcg
    42241 aataaccgct taagctgcct gctaacagtt tgccaaacca gctaataaaa ccagtgttgt
    42301 tgagtccggt agcgagggtg ataagcgagg ccagccagaa gaaaacgttc cacgcggctt
    42361 tattactgac aatgtcgtcc caactgataa tgcgcagaag cagcatcagt gccaccacgc
    42421 tgtaaccgac catcgcggca tcgatataat caccgccgaa aatccacagc accagcgcgc
    42481 ctaccatcag ccccagcatc cgtttttcac gcgaacagag cgggcccatt gcctgcagtt
    42541 ccgtctctgc ccagcgcggc acctgatcgc ctgacttcag taccggcggg tacagcacat
    42601 aagccagcca gggaaccagc agcaccagca aaatactgag cggcaacatc ccgaggaacc
    42661 agtcgcccca actcagcgtg gcgtgagatg cgcttttcat cagtccaatt aacagcaagt
    42721 ttggtgccat cgccgtcaag aaaatggcgc tggtcacgca gtcggcaaca atccccatcc
    42781 acatgatgta cgagccaatg ctgcgcgaac tgctgtcgtt tggttgtgat tgatagagcg
    42841 gtggcaggtt acggatgatg ggatagataa tccccgcacc acgcgccgag ttggacggtg
    42901 ttacaggtgc gaggatcagc tcggagaaca tcaccgcata gccgagaaac agcgtgcgat
    42961 gtcccatctt tttcaccaga atcagcgcaa tgcggcgtcc cagcccggtt ttttcatagc
    43021 ctgtgccaaa cataaaggcg gcgaaaatca gccagataac cgaattagaa aaaccagaaa
    43081 ccgcccacga gagggatttt gcagtgaatt taaagcctgg ctgggcgagc tgttcggggc
    43141 tgaacagcag ccagggagag agtatggcga tgatggagat acccaccatc gccaccacgg
    43201 cacccggcac gggttcgagg atcagtccaa cgatcacgcc agtaaaaacg gcaaagtaga
    43261 gccaggtatg actctccagc cctgcgggaa ccggaattag agcaataatg gcgatgaccg
    43321 ccagcggcgc aaggtatcgc caccattcag tggaaggttt catcgtgtta tgcctgcgaa
    43381 aaaggagagg gggcgagcct ctccgggagg gttatttgat gtaatggacg tgctcgcaga
    43441 tctcttccac gatgggatcg cggcgttcgg cgaacagctt tttgttttcg gctatcaggt
    43501 tgttgccgtg ggtatcaata gagacaatca gcgggccgaa ctctttgacc cggcagaccc
    43561 acagtgactc cggcatcccg agttctgtcc agtgcacttc ttcaatctct tccacctggg
    43621 ttgccgccag caccgcgcag cctgccggga aaatcacatg tagcgccttg aatttctggc
    43681 agccttcttc ggtcagcggc cccattccgc ctttgccaac caccagtttc acgccggtct
    43741 gctcgataaa ttcacgttca aaactttcca tacgcatact ggttgtcggg ccaacggaaa
    43801 ccatctccca tttatcgccg tttttgcgca cgatggggcc agcgtggaaa atcgctttgc
    43861 cgttgagatc gtaagggatc ggacgtttca gttcgatcaa gcggcggtga caaacgtcac
    43921 ggcaggtcac cagcgtaccg gtcaggtaga tcacatcgcc gacgcgaata tcttgcagat
    43981 cttcagcttt gatcggggtt gtcaggatct ttttcataac gcgctccggg tgtgagacag
    44041 attttcaaag gtgagatcgg catgaaccag cagcgtgccg cgacgatgcg cccagcagcc
    44101 ggtagagaca gcaacgccga tggttgacgg atggcgggcg gcagattcga tatgcacgcc
    44161 catcactgaa ctgttgccgg tcagcccttg tggaccaatc cccagacggt tgagtccttc
    44221 ttccaggcgt agctccagct ctgccgcttt tggattggga tggcggctgc caatcggacg
    44281 caaaatggct ttacgcgaga gtacggcggc ggtttccacc gaggtggcga tgcccacgcc
    44341 caccagtacc ggtggacagg cgtttacggc gagggtggag atattttcga agacgaattt
    44401 caccacgcct tcgtagcctt ctgacggcat taacactttc gagcggccag gtagcgtgca
    44461 gccgccgcct gccatgtaaa cttcgatttc tgcatcgtca ttgtcgggga tgatgtccca
    44521 ggtgacccac ggtacgccgc taccggtatt tttgccggtg tttacttcgt caaaaatttc
    44581 taccgcattg tgacgtagtg gcgctttgac ggttgcctct tccacggctt gtttgagtat
    44641 gctttgcagc tcgccaagca gtgggaagcg ggaaccgact ttaacgaaga acataatctc
    44701 cccggtgtcc tgacaggcag gacgattcag gtcaatcgct ttttgcatgt tgtcgaacat
    44761 cgtatggtag ataattttcc ccatcgacga cgtttcggca tcctttagct gttttagttt
    44821 atccaccacg tcatcaggca ttcgggtaga aatcatggcg gtaaagttag cgacaatctc
    44881 tgtcaactta ttcactgcct gttgcttatt actttcgctc atcatggcaa ctccagtttt
    44941 tgtatttgtc tggaaaaatt caccgccaca aactaccact aaaaatgtgg ttttatattt
    45001 tttgccaccg tgaatatgta attaacagtt cgtgaatcaa cgcgaaaata agaatgaata
    45061 ttgcgccaga gcagggaatg gcaatgtttt gcgaaataca ctgcgcttat cacaactgcg
    45121 gataattaac gatgctgaat agctggcctt tagccaaaga cttgcaggta ctggtagaaa
    45181 tcgtccacag cgggagtttt agtgctgcag cggcgacgct tgggcaaacg cctgcatttg
    45241 tcaccaagcg gatccaaatc cttgaaaata cgctggcaac aacgctgctg aaccgctccg
    45301 ctcgcggcgt ggcgctgact gaaagtggcc aacgttgtta cgaacatgcg ctggaaatcc
    45361 tcacccaata tcagcgactg gtcgatgacg tcacgcaaat caaaacgcgc ccggagggga
    45421 tgattcgtat tggttgcagt tttggttttg ggcgcagcca tattgcgcca gccattaccg
    45481 aacttatgcg caattatcct gagttacagg tgcattttga attgttcgat cggcaaattg
    45541 atttagtgca ggataatatt gatctggata ttcgtattaa tgacgaaatt cctgattatt
    45601 atattgcgca tctgttaacg aaaaataaaa gaatattatg cgcagcacct gagtatctgc
    45661 aaaaatatcc gcagccacaa tcattacagg aattaagtcg tcatgactgt ctggtgacca
    45721 aagagcgcga tatgacccac ggaatatggg agttggggaa cggtcaggag aaaaaatcgg
    45781 tgaaggttag cgggcatctt tcctccaata gcggcgagat agtgttgcaa tgggcgctgg
    45841 agggcaaagg gattatgctg cgttccgagt gggatgtgtt gccgtttctg gagagtggca
    45901 aactggtgca agtattgccg gagtatgcac aaagcgccaa tatctgggct gtttaccggg
    45961 agccgctcta tcgcagcatg aaattacgtg tctgcgtgga atttctggcg gcatggtgcc
    46021 agcaacggct gggcaagccc gatgaaggct atcaggtcat gtagatccac cagaaatcac
    46081 tcgggatcct tttcgcgctt tcttttgaat ttcgtccaga tttttgtctc ctgacgacgc
    46141 cacagacgct ggatgttgtc atgatgacgc agcaggatca ggcaagagag catcgaaacc
    46201 gggaaggtga attgtggctt aaaccaccat acataaaacg gagcaatcag tgcactgaca
    46261 atcgctccca gcgacgagta tccgctcaat agcacggtca gtaaccaggt tcccgccatt
    46321 actccggtga gatcccagcc aatgggtgcg atggcaccaa aagcggtagc aacgcctttt
    46381 cctcctttaa atccgaagaa aacgggccag atgtgtccaa gacaggcggc gatggcaatt
    46441 aagcctagcc agaaggggct gacacctaat tcatacgcgc cccagacggg caacattcct
    46501 ttcagaacgt cgaaaatcag tactgctacg gctgctccct tgccaccgat acgtaacaca
    46561 ttggttgcgc ctggattgcc ggagccgctg gttcgcggat cgggcagccc acacaagcgg
    46621 caaaccagaa tggcactgga aatggagccg cagaggtacg cgatgaggat cattccaggc
    46681 gcgattgcac tcataagctg ttccgttttg aaaattcgtg ttcaacgatg aatctgtgga
    46741 taatacgcac atttcgccgg aagtggtatc cggttagcca aaaagcaggc aggacgtgat
    46801 ggatattgta tttatagagc aactttcggt aatcaccact attggtgttt acgactggga
    46861 acagaccatc gaacagaagt tagtgttcga tatcgaaatg gcatgggata accgtaaagc
    46921 ggcgaaaagt gatgatgtgg cggattgcct cagttacgct gacattgcag aaacggtggt
    46981 cagccacgtc gagggggcgc gttttgcgct ggtggaacgc gtggctgaag aggtggcgga
    47041 gctgctgtta gcacgcttca actcgccgtg ggtgcgtatc aaactcagca agccaagcgc
    47101 agtggcgcgg gcggcgaatg ttggcgtaat cattgagcgt ggcaataatc tgaaagaaaa
    47161 taattaattt tacagctgtt aaaccaaacg gttataacct ggtcatacgc agtagttcgg
    47221 acaagcggta cattttaata atttaggggt ttattgatga gcgatatgca ctcgctgctg
    47281 atagcggcaa tattgggtgt ggtcgaagga ttgacagaat ttctgccggt atccagcacg
    47341 ggccatatga ttattgtcgg tcacttgttg gggtttgagg gcgacacggc gaaaaccttt
    47401 gaagttgtga tccagttagg atcaattctg gcggtagtag tgatgttctg gcggcgtctg
    47461 tttggcctga ttggcatcca ctttggccgc ccgttgcagc acgaaggtga aagcaaaggt
    47521 cgtttaacgc tgatccacat tttgctgggg atgattccgg cggtggtact ggggctattg
    47581 ttccacgaaa cgattaagtc attgtttaac ccgataaatg tgatgtatgc gctggtcgtt
    47641 ggcggtttgt tgctgattgc cgccgaatgc ctgaagccga aagagccgcg tgcgccgggt
    47701 cttgatgata tgacctatcg tcaggcgttt atgattggct gtttccagtg tctggcgctg
    47761 tggccgggtt tctcccgttc cggggccacc atttcaggtg ggatgctgat gggcgtgagc
    47821 cgttacgctg cttccgagtt ctcgttcctg ctggcggtac cgatgatgat gggcgcaacg
    47881 gcgctcgatc tctacaaaag ctggggcttc ctgacaaccg gcgatatccc gatgtttgcc
    47941 gttgggttta tcaccgcttt tgtggtggcg ctgatagcga ttaaaacctt cctgcaattg
    48001 attaagcgca tttcgtttat cccgttcgcc atttatcgct ttattgtggc ggctgcggtg
    48061 tacgtcgtgt tcttttgatt gagaaaaggt tcgggagcag gcctggtttt ctccctcgct
    48121 ctgtagggag agggccgggg tgggggtgat tgttcgcacc ggtgctgg
//
LOCUS       DAZGSP010000028        48168 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZGSP010000028 DAZGSP010000000
VERSION     DAZGSP010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.028"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..362)
                     /locus_tag="ACHK74_001737"
     CDS             complement(<1..362)
                     /locus_tag="ACHK74_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP0220575.1"
                     /translation="MGVRGTHQPSAHASGSQANLRPTACELINADCLEFIRSLPENSV
                     DLIVTDPPYFKVKPEGWDNQWTGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLAS
                     DIEIMMLERFSVLNHIIWA"
     gene            complement(395..592)
                     /locus_tag="ACHK74_001738"
     CDS             complement(395..592)
                     /locus_tag="ACHK74_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP0220576.1"
                     /translation="MLKQQDMTETARAVFNELSATEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(766..1479)
                     /locus_tag="ACHK74_001739"
     CDS             complement(766..1479)
                     /locus_tag="ACHK74_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220577.1"
                     /translation="MDDSALLRNSSLFVAYMGCLGWGSAYFYGWGTSFYYGFPWWVVG
                     AGVDDVARSLFYAVTVIVIFLIGWGVGIVFFLGIKQKRNIQNLSFIRLFLAILLLFIP
                     PVLEFSVIHQHVEPDVLIFCVLAAFAITLFVRSGRRLVSVKCFSEMSVIRHHRIEFMM
                     AGFMIYFWAFSLIAGWYKPQFKREYQAVHYENVWYYIIARYDDRLVLSKSYRSGGKKF
                     VIFNSGNINDFEINTVRVR"
     gene            complement(1733..2398)
                     /locus_tag="ACHK74_001740"
     CDS             complement(1733..2398)
                     /locus_tag="ACHK74_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP0220578.1"
                     /translation="MNNQYLQFVREQLMIATADLSGSTKGQLEVWQENALFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGSSIALVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEHQIAITQWAWTEFNAQSGTRKIAGKTLVRLKTLIWLAAQAVKAE
                     LFGGEGYEYQELALLVGVTTKNWSKTFTGHWVAMKHIFHRLDSEALLLVEGTRSKQKA
                     AFS"
     gene            complement(2391..2753)
                     /locus_tag="ACHK74_001741"
     CDS             complement(2391..2753)
                     /locus_tag="ACHK74_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP0220579.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVVLIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGK
                     PVSGGRLEIRITEVGVHE"
     gene            complement(2766..3815)
                     /locus_tag="ACHK74_001742"
     CDS             complement(2766..3815)
                     /locus_tag="ACHK74_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0220580.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEAEPKNMRD
                     LPSGVVPAVRQPLAEDKTLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLVAWMIDVIRHAISGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGVGTKAHDIFTLPLCREHHNELHADPLKFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
     gene            complement(3817..4089)
                     /locus_tag="ACHK74_001743"
     CDS             complement(3817..4089)
                     /locus_tag="ACHK74_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220581.1"
                     /translation="MAQHIKSHNSEVDPAIKRGRRSHVFKTDWYQHPPCTEEQAEWLI
                     QCYRRRGYEFEKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(4258..4413)
                     /locus_tag="ACHK74_001744"
     CDS             complement(4258..4413)
                     /locus_tag="ACHK74_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0220582.1"
                     /translation="MKQQKAMLIALIVICITVVVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            complement(4704..5201)
                     /locus_tag="ACHK74_001745"
     CDS             complement(4704..5201)
                     /locus_tag="ACHK74_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220583.1"
                     /translation="MRSHISKALSGEKMKKKDLDADLRWIHGVIVQAAWFVKASLEQN
                     VLTGNSQVIPDDGREQFETLIRFHADDKNHETLLLRANEGMNYRDPNVDLAWIFWKSS
                     REHILPGKSDNNPASGDQVSELTMWVKRLSHSLKSVNKSSKLPDKAMDYLKRNGLINE
                     ENILR"
     gene            complement(5259..5405)
                     /locus_tag="ACHK74_001746"
                     /pseudo
     CDS             complement(5259..5405)
                     /locus_tag="ACHK74_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5414..5770)
                     /locus_tag="ACHK74_001747"
                     /pseudo
     CDS             complement(5414..5770)
                     /locus_tag="ACHK74_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="valyl-tRNA synthetase"
     gene            complement(5920..6225)
                     /locus_tag="ACHK74_001748"
     CDS             complement(5920..6225)
                     /locus_tag="ACHK74_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP0220584.1"
                     /translation="MRVSIYIAGPMTGYENFNREAFHRAEEALKREGHTVLNPAVLPD
                     GLTQPQYMDICMAMIRCVDAVYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSERN"
     gene            complement(6222..6503)
                     /locus_tag="ACHK74_001749"
     CDS             complement(6222..6503)
                     /locus_tag="ACHK74_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0220585.1"
                     /translation="MILRQCAGTMKVKSIGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVQA"
     gene            complement(6536..7252)
                     /locus_tag="ACHK74_001750"
     CDS             complement(6536..7252)
                     /locus_tag="ACHK74_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP0220586.1"
                     /translation="METVFDALKALKRASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLTQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVAETDGKAFPQPAGVALPVQETT
                     TQEEMKTEIVEDIVKLQPSVTETKADDLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILWDITATREQQR"
     gene            complement(7286..7747)
                     /locus_tag="ACHK74_001751"
     CDS             complement(7286..7747)
                     /locus_tag="ACHK74_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP0220587.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPKGPTPIERLKQEYERRKAAGFI"
     gene            complement(7740..8777)
                     /locus_tag="ACHK74_001752"
     CDS             complement(7740..8777)
                     /locus_tag="ACHK74_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP0220588.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSISALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGTVNKNSTENGYVSAQNRPKNGTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLDGLSEPIGKFSMTTVWQPSLDFRQRAV
                     VWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVS
                     HAASGGTASRAVQEIRAAREQWERENGFISNGNGLEAVGAYGGGVFEPLDSEEWGRTF
                     EALDCPDWCDD"
     gene            complement(8749..9300)
                     /locus_tag="ACHK74_001753"
     CDS             complement(8749..9300)
                     /locus_tag="ACHK74_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP0220589.1"
                     /translation="MKITPEQVCEALDAWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKREKFTALIPAIMEAIRVSDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKRAYRQQY
                     QHYFADKQISVWGSYEYRCTTMG"
     gene            complement(9284..9550)
                     /locus_tag="ACHK74_001754"
     CDS             complement(9284..9550)
                     /locus_tag="ACHK74_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0220590.1"
                     /translation="MSHTTLADVIKAVRVSVVADVCGVSQRAIYKWMNNGKLPRTEYT
                     GETNYAEKIALASNGLFSADAVLTIGRIKPTANESLAVNNENHP"
     gene            9630..10049
                     /locus_tag="ACHK74_001755"
     CDS             9630..10049
                     /locus_tag="ACHK74_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0220591.1"
                     /translation="MVHEDEARKEFASRLALACENAGFEQHGRQAEIARRMKLTPKAV
                     SKWFNGEAIPRREKLKELAALIGTTPTYLLGEDTEESGSLRFYQELSPKQKIIIELLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            10345..10497
                     /locus_tag="ACHK74_001756"
     CDS             10345..10497
                     /locus_tag="ACHK74_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP0220592.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTRSKTFKTEAGARRW
                     LARNSD"
     gene            complement(10611..11126)
                     /locus_tag="ACHK74_001757"
     CDS             complement(10611..11126)
                     /locus_tag="ACHK74_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220593.1"
                     /translation="MIAELTAAMTAIRETAQITKLMNEAKTQAEVNAAIGELNSKLAS
                     IQHECVSLVELVSTYQEINASLKAKIAEFENFEAQTEGYILNQLESGTFVYSKEVTVN
                     GGSIIMHLCPKCFGQKIVSILQPFPVREHEFFHKSRCLYCENQFLMNKNPDYVSPPSI
                     EELARKLNGNL"
     gene            11655..11843
                     /gene="dicB"
                     /locus_tag="ACHK74_001758"
     CDS             11655..11843
                     /gene="dicB"
                     /locus_tag="ACHK74_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP0220594.1"
                     /translation="METSLPNVNTSEGCFNIGILLSNREFTEDAIRMRKYEPYLLNDN
                     SILSRIALLELGIFGGQQ"
     gene            11840..12028
                     /locus_tag="ACHK74_001759"
     CDS             11840..12028
                     /locus_tag="ACHK74_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP0220595.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYASKESCHQARDEQKISGE
                     CLPLNKVSLYLNNEIPAG"
     gene            12124..14595
                     /locus_tag="ACHK74_001760"
     CDS             12124..14595
                     /locus_tag="ACHK74_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP0220596.1"
                     /translation="MSKIFICAAIPDEQAIKNEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGTPRPALDSWDAEYMLENRWDEASASFVPVETESDPM
                     NVTFDKLSPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPEIKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKKTGKYTSVVDLART
                     RVNQHLTENSAAKINPVTTAIRREYRQTWKTLDKELACALWPGDVDTGNIDGTIHRWA
                     KNEVIDKDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSADAILSSAAQTDPVETAESNSQKNEILVEAEPSVEREGPFYF
                     VFTDKDGEKYGRANKLSGLDKALAAGGTEISKEEYFARKNGTYTGLQQNTDTAEDSEQ
                     PEPVKVTADEVNKIMQAANISQPDTDKLLAASRGEFVEGISDPNDPKWVKGIETRDSV
                     NQNQPESEQNDQKAEQNSPNALQNEPETKLPEPEVQQEPEKVCTACGQTGDGNCPDCG
                     AVMGDATYQETFDEKNQAEVQEDDSEEMEGAEHPNNENAGNDQHHTSDSETGEAAEPL
                     IAVNGHHIITSTSRVWIHLSVDLETMGTNPDAPINSIGGKFFDPATGEMGPEFSKAID
                     LETSGGIIDRKTIKWWAKRSREAQSAIFTDEISLDVALRLFIEFIEKNSGGRFVQVWG
                     NGANFDNVILRRSYERQGIPCPWLYYNDRDVRTIVELGNAIGFDVRMAIPFEGVPHNA
                     LDDARHQAKQVSAIWQKLIPSQADF"
     gene            14660..14908
                     /locus_tag="ACHK74_001761"
     CDS             14660..14908
                     /locus_tag="ACHK74_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP0220597.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPGTLSKYAKAGMIFPLPKKVGRH
                     WRVDPRARFVGMVNKPEVIATDHPALKRILEDGAPTKI"
     gene            14886..16016
                     /locus_tag="ACHK74_001762"
     CDS             14886..16016
                     /locus_tag="ACHK74_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFP0220598.1"
                     /translation="MARPRKYKTEVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKQAEAIAAEANSRLARQQMEQMLTLQEKIIRDTGGSSTVSVFLNSYRKIQQERYENG
                     EIKLNTLKQKASPLRVFDERFGTRPLDAITVKDVVSVLEDYKAKGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMIIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMKFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGIDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(16062..16715)
                     /gene="ompW"
                     /locus_tag="ACHK74_001763"
     CDS             complement(16062..16715)
                     /gene="ompW"
                     /locus_tag="ACHK74_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFP0220599.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSDGRKLAKGSYSDNVRLDPWVFMFSAGYRF"
     gene            16955..17044
                     /locus_tag="ACHK74_001764"
                     /pseudo
     CDS             16955..17044
                     /locus_tag="ACHK74_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            17072..17815
                     /gene="yciC"
                     /locus_tag="ACHK74_001765"
     CDS             17072..17815
                     /gene="yciC"
                     /locus_tag="ACHK74_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFP0220600.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            17845..18384
                     /gene="yciB"
                     /locus_tag="ACHK74_001766"
     CDS             17845..18384
                     /gene="yciB"
                     /locus_tag="ACHK74_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFP0220601.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            18489..18887
                     /gene="yciA"
                     /locus_tag="ACHK74_001767"
     CDS             18489..18887
                     /gene="yciA"
                     /locus_tag="ACHK74_001767"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFP0220602.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(18927..19646)
                     /gene="tonB"
                     /locus_tag="ACHK74_001768"
     CDS             complement(18927..19646)
                     /gene="tonB"
                     /locus_tag="ACHK74_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFP0220603.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            19870..20166
                     /gene="yciI"
                     /locus_tag="ACHK74_001769"
     CDS             19870..20166
                     /gene="yciI"
                     /locus_tag="ACHK74_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFP0220604.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            20465..21718
                     /gene="kch"
                     /locus_tag="ACHK74_001770"
     CDS             20465..21718
                     /gene="kch"
                     /locus_tag="ACHK74_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFP0220605.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(21773..21946)
                     /gene="yciY"
                     /locus_tag="ACHK74_001771"
     CDS             complement(21773..21946)
                     /gene="yciY"
                     /locus_tag="ACHK74_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFP0220606.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            22089..23549
                     /gene="cls"
                     /locus_tag="ACHK74_001772"
     CDS             22089..23549
                     /gene="cls"
                     /locus_tag="ACHK74_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP0220607.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASDPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            23584..23913
                     /gene="yciU"
                     /locus_tag="ACHK74_001773"
     CDS             23584..23913
                     /gene="yciU"
                     /locus_tag="ACHK74_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFP0220608.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(23966..24970)
                     /gene="oppF"
                     /locus_tag="ACHK74_001774"
     CDS             complement(23966..24970)
                     /gene="oppF"
                     /locus_tag="ACHK74_001774"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFP0220609.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGIT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(24967..25980)
                     /gene="oppD"
                     /locus_tag="ACHK74_001775"
     CDS             complement(24967..25980)
                     /gene="oppD"
                     /locus_tag="ACHK74_001775"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFP0220610.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(25992..26900)
                     /gene="oppC"
                     /locus_tag="ACHK74_001776"
     CDS             complement(25992..26900)
                     /gene="oppC"
                     /locus_tag="ACHK74_001776"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFP0220611.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFIHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(26915..27835)
                     /gene="oppB"
                     /locus_tag="ACHK74_001777"
     CDS             complement(26915..27835)
                     /gene="oppB"
                     /locus_tag="ACHK74_001777"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFP0220612.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(27921..29552)
                     /gene="oppA"
                     /locus_tag="ACHK74_001778"
     CDS             complement(27921..29552)
                     /gene="oppA"
                     /locus_tag="ACHK74_001778"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFP0220613.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            29836..30132
                     /locus_tag="ACHK74_001779"
     CDS             29836..30132
                     /locus_tag="ACHK74_001779"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220614.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(30290..30937)
                     /gene="ychE"
                     /locus_tag="ACHK74_001780"
     CDS             complement(30290..30937)
                     /gene="ychE"
                     /locus_tag="ACHK74_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFP0220615.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(30968..31021)
                     /gene="ychE"
                     /locus_tag="ACHK74_001781"
     CDS             complement(30968..31021)
                     /gene="ychE"
                     /locus_tag="ACHK74_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFP0220616.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            31414..34089
                     /gene="adhE"
                     /locus_tag="ACHK74_001782"
                     /gene_synonym="adhC"
     CDS             31414..34089
                     /gene="adhE"
                     /locus_tag="ACHK74_001782"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFP0220617.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(34391..35008)
                     /gene="tdk"
                     /locus_tag="ACHK74_001783"
     CDS             complement(34391..35008)
                     /gene="tdk"
                     /locus_tag="ACHK74_001783"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP0220618.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            35613..36026
                     /gene="hns"
                     /locus_tag="ACHK74_001784"
     CDS             35613..36026
                     /gene="hns"
                     /locus_tag="ACHK74_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFP0220619.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(36170..37078)
                     /gene="galU"
                     /locus_tag="ACHK74_001785"
     CDS             complement(36170..37078)
                     /gene="galU"
                     /locus_tag="ACHK74_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP0220620.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(37280..38293)
                     /gene="rssB"
                     /locus_tag="ACHK74_001786"
     CDS             complement(37280..38293)
                     /gene="rssB"
                     /locus_tag="ACHK74_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFP0220621.1"
                     /translation="MTQPLVGKQILIVEDEPVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(38385..39290)
                     /gene="rssA"
                     /locus_tag="ACHK74_001787"
     CDS             complement(38385..39290)
                     /gene="rssA"
                     /locus_tag="ACHK74_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFP0220622.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            39403..39861
                     /gene="ychJ"
                     /locus_tag="ACHK74_001788"
     CDS             39403..39861
                     /gene="ychJ"
                     /locus_tag="ACHK74_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFP0220623.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            39911..40753
                     /gene="purU"
                     /locus_tag="ACHK74_001789"
     CDS             39911..40753
                     /gene="purU"
                     /locus_tag="ACHK74_001789"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFP0220624.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            40913..40997
                     /locus_tag="ACHK74_001790"
     tRNA            40913..40997
                     /locus_tag="ACHK74_001790"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40947..40949,aa:Tyr,seq:gta)
     gene            41045..41176
                     /locus_tag="ACHK74_001791"
     ncRNA           41045..41176
                     /ncRNA_class="other"
                     /locus_tag="ACHK74_001791"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(41360..42037)
                     /gene="narI"
                     /locus_tag="ACHK74_001792"
     CDS             complement(41360..42037)
                     /gene="narI"
                     /locus_tag="ACHK74_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP0220625.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(42037..42747)
                     /gene="narJ"
                     /locus_tag="ACHK74_001793"
     CDS             complement(42037..42747)
                     /gene="narJ"
                     /locus_tag="ACHK74_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP0220626.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(42744..44282)
                     /gene="narH"
                     /locus_tag="ACHK74_001794"
     CDS             complement(42744..44282)
                     /gene="narH"
                     /locus_tag="ACHK74_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP0220627.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(44279..48022)
                     /gene="narG"
                     /locus_tag="ACHK74_001795"
     CDS             complement(44279..48022)
                     /gene="narG"
                     /locus_tag="ACHK74_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFP0220628.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMTDKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT        12341 a        12308 c        11205 g        12314 t
ORIGIN      
        1 gcccagataa tatggttcag cacactgaag cgttcaagca tcatgatttc gatatcagat
       61 gccaggcgat ggccacagaa caggtaaaga cttccggcag gtttcagcac ccgccagaac
      121 tgcgccagac actggtccag ccacttcagg taatcctcat cacccgtcca ctggttatcc
      181 cagccctcag gcttcacttt aaagtacggc gggtccgtga ctatcaggtc aacagaattt
      241 tcgggtaacg accggataaa ttccaggcag tcggcgttga ttaactcaca agcagtgggc
      301 ctgaggtttg cttgtgatcc ggacgcatga gcagatggct ggtgagtgcc cctaaccccc
      361 accagccgcc catttaccac aaataaaaaa gccttcagga ctgaaggcgt ctgtaacaac
      421 cgaactgata gtctgccaga cccgccataa caagctgggt cagtattagc tggcagcgtt
      481 cgcgtgaaag gtacgtattc tgtgcaatct ccccgactgt cgccggttcg gtggcactta
      541 attcattaaa aactgctctg gcggtttctg tcatatcctg ctgtttcagc atgtcttttt
      601 cccttttccg gttaacgtga cacaccaata actcttgtca aaaaagccag caagctgaaa
      661 gaccggtatt aataaccacc tgcacatttt atgtactgca ccatttttct ggcataaaaa
      721 aaccgctcaa tgccgggcag taaaatcttt attactcagg aaattttaac gtactctgac
      781 agtattaatt tcaaaatcat taatatttcc gctattaaat ataacgaatt tcttaccccc
      841 actcctgtat gatttcgata acaccagacg atcatcatag cgcgcaataa tgtaatacca
      901 tacattctca tagtggaccg cctgatattc cctcttaaac tgtggtttgt accaaccggc
      961 aataagagag aatgcccaga aataaatcat aaacccagcc atcatgaact caattcggtg
     1021 atggcgaata acagacattt ccgaaaaaca tttgactgaa acaagtcttc ttccagacct
     1081 gacaaaaagc gtgattgcaa aggcagcaag aacgcagaaa atcagtacat ctggctcaac
     1141 atgctgatga attacagaaa actccagaac aggtggaata aaaagcagca atatcgcgag
     1201 aaaaagccgg ataaaactca aattttgtat attgcgcttt tgttttatgc ccaaaaagaa
     1261 aacaatacca actccccatc caataaggaa tataacaata actgtcacag cataaaacaa
     1321 acttcgtgct acatcatcga caccagcccc gacaacccac catggaaagc catagtaaaa
     1381 tgaagtaccc catccataga aataagcgct cccccatccg agacagccca tataagcaac
     1441 aaaaagtgaa gagtttctga gcagagcact gtcatccata gtaacaccat taacaactca
     1501 aaaatatcaa cacacattac ataacaaatt ggattccatg cagtcaaggg gcgtcattga
     1561 tggagaaagt attggcacaa tcgtcatcac gtttaatgtc tatgccattt tttggggggt
     1621 aaaaaacccg ctcggtcacg ggttttagtg gctttgccat cacgtataat aacggcaaaa
     1681 tatcagattt acacgaaata tatgcctttt tatctacttt tgcaatactt tgctatgaaa
     1741 atgccgcctt ttgttttgaa cgtgttccct ccaccaacaa taaagcttca ctatccagcc
     1801 gatgaaaaat gtgtttcatt gcaacccagt gaccagtaaa tgtcttggac cagtttttgg
     1861 ttgttactcc caccagtaac gccagttcct ggtattcgta accttcccca ccaaaaagct
     1921 cagcttttac cgcctgcgcc gccaaccaga tcaacgtctt caggcgcacc agagtttttc
     1981 ctgcaatttt tctggtaccg gattgagcat taaattcagt ccacgcccac tgcgttatcg
     2041 cgatctgatg ctcccagcaa atgctaccgc tgtaacacca cagcagccag gctttatgat
     2101 gttcttcaag agacagaacg gcgcgtcgcc atgatgatgt cgaaaactca accggactga
     2161 ccagggcaat tgatgagcct ttcgccagtg attgtttacc cgggatcggg ggattatccc
     2221 gcgtgatcat ttttccggtt acctcatcgc ggtaacgaat ttttttgcgt ctgtaacgcc
     2281 ctgtatcgaa cagggcattt tcctgccaga cttccagctg gccttttgtc gacccactaa
     2341 gatccgcagt ggcaatcatg agttgctcac gaacaaactg taaatactgg ttattcatgc
     2401 acacccacct ctgtaattct tatctccagc cgtccaccag atactggctt gccacgtaca
     2461 atattgattt catcaaactg ctcatcgtcc attaacaacc ccgcgtgcgt cagcgcatcc
     2521 agcggtgctt tcagaatatt gtccaggtca cggcggcgct tatccggcgg ctctgcaata
     2581 atttttattg ccagccgtcc ggacaggctt aatttcagtc gctgctggcg aacaataagc
     2641 accactgccc ggcgataacg ctccccggct tttgatacaa aatatgtgct gccacggcgt
     2701 cgccagtaag tgttcaccgt cggcgggtaa ggtaaaacca aatctatgag catcagtcac
     2761 ctcttttacc cgagcacgcc agtcgcaaag gcgtgatcaa gaaaacgaaa aattaactca
     2821 atctgagagc cgtacttttt ctcaaacttc agcgggtctg catgaagttc gttgtggtgc
     2881 tcccggcaca acggtagcgt gaaaatatcg tgggcctttg ttcccactcc cccctgacca
     2941 tgaccaatca ggtgatgcgg atcgtcagct ggcttaccac aacacgcaca cggctgtgtc
     3001 tttacccagc gcgtgtattt ctcattaacc caacggcgac gtttaggccg cctcatgaac
     3061 gattcaggag actccggatc aacggcgata ctgacaaccg tttttttctg tggtggattt
     3121 tgttgctggt ggacgtgaag tggcagcgca atattttttg tgcgctgctt cagtatgctg
     3181 atggctgtct gttctcccgg tacgatgtca ctctcacggt atacggagcg gattttttcc
     3241 gccggtaatc ccagcgaacg acgcgctact gcctcaggta gtgcatccac cacctgattg
     3301 caggccgccc accaggataa ttcggccagc gataactccc tctcctgcgt accgcttatt
     3361 gcgtgacgga tgacgtcaat catccaggca accagattct gctgagcaag ttgatcgagt
     3421 gattctgatg tctggtcgcg cagctggttg tcacagtgcc agcacaatac catcgcgccg
     3481 gtaccgtaac ggtgaatgac tgtttcgctg tgatgataat cgccgtgtgg ccactggcag
     3541 gatttcacgt gacgtaataa ccagtcagac agtgcacctg caccacctgc tgcacgaata
     3601 acccgctcat cgctgaaaaa tggcagtaat gttttatcct ctgccagcgg ctggcgaacg
     3661 gcaggaacga ctccggacgg cagatcgcgc atgtttttcg gttccgcctc caccaatatt
     3721 ctgccgttat ggaatgctga cattgattca cggcctggct taacgataac cagaccgagt
     3781 tccggtacca gaacaggtcg aagtaatacc cgcacgttac ctccagatgc gctgctggaa
     3841 tgtgcgggac ggacgcggtg ggcgttcgga ataagggagc ctgacggaga ttatccagtg
     3901 acgataatcg aggctgaggg ctttctcaaa ctcatatccg cgcctgcggt aacactggat
     3961 cagccattcg gcctgttctt cagtgcatgg gggatgctgg taccagtcag ttttaaatac
     4021 gtgcgaacgc cgcccccgct taatggccgg atcgacttca gaattgtggg attttatatg
     4081 ttgcgccatc gggttctccg gtgacgcagc aggtgccagt tgttcaggct gacgtgcgaa
     4141 ttgtaaacca gaatgccagg aaaaaacaaa gtctgccgaa acggggtaag tgcgggtgcg
     4201 ttgaggatgc ctgacacatc agaggtggcg agggatttct ccctcgcttg gtcactctta
     4261 cttctcagat tcgtagtcta cgaagacagc gacctccgtc tggccggttc ggattcgtac
     4321 ctcgcagagg tctttcctcg ttaccagtgc cgtcacaaca acggtaatac agatgacgat
     4381 cagggcgatt aacatcgcct tttgctgctt catagcctgc ttctccttgc ctttcggcac
     4441 gtaagaggct aacctacgtt tgtgaagcat agattgggcc tcagattaat gttaagcgtc
     4501 ctgcaagacg cgtaatgtta actggggctt ttctctgtct gccttacggc ggcatgcccg
     4561 aggcagacag cctcaagcac ccgcagcaat tctacttaac tcttctttcc ccgcaaaccg
     4621 tttttatccc cagcggcaaa tcgaatacac caccagcgcc accgccattg caattcctac
     4681 cgttatgaac gcttcaggcc aggtcatcgt aaaatattct cctcgtttat cagtccgttt
     4741 cgcttcaggt agtccatcgc tttatccggt agtttgcttg atttattaac gctttttaag
     4801 gaatgagata accgcttcac ccacattgtt aattcactta cctgatcgcc tgatgctgga
     4861 ttattgtcgc tttttccagg caatatgtgc tctcgactgg atttccagaa tatccatgcc
     4921 aaatccacat ttggatcccg gtaattcatc ccttcgttag cacgaagcaa taatgtttca
     4981 tggtttttat catctgcatg gaacctgata agtgtttcaa actgttcgcg accgtcatca
     5041 gggattacct gagagttacc cgttagcaca ttctgctcca gtgatgcttt tacaaaccag
     5101 gcggcctgga ctataacccc atggatccag cgcaaatcag catcgagatc cttcttcttc
     5161 atcttttcgc cacttaaagc cttgcttatg tggctgcgca ccaggtcttc atgtaactcc
     5221 ttcgcctcct caatggtgaa accgccaggc aaacgccccg gagttaccgg agggttgcca
     5281 gccttgcgca tggcaatctc catgatttca accatatctc ctggtggaat tttacaatgc
     5341 tgaccaatat gcctctgctg cccggcatat tcgaggatgt gctccagttt gatacgatta
     5401 atcatgattt atctccatta agcatggcat cccggcaggc attccagcct tcatcaaatc
     5461 cgacaatgcc attatttaaa gatggacgag catccggcac caccggtaca ggttgggcta
     5521 catataacgg ctgaacatac cagccctttg ataaccaact atcagcaacg tttttactcc
     5581 tggttattgc tggaataccc aggccattgt ctgaatgcag ccatgccacc ggaaccgcct
     5641 ccagcgatgc cagtgcaatt ttgaatagct ctccctctac cctggccatc tctgaattag
     5701 ggtaacattt cgcaaccgct atttttaatt tggcttcttt gattaattgc tcttttgtta
     5761 attcagtcat ttttcattac cgccctttct ggcggtctcc tgatgttctg agggtgcaga
     5821 aatccctccg gttaaggatt aaattttatt tacagtgctg attttaatta ttcagatctg
     5881 gattatgctt tctctttcac ctgccgtagc tcctggtaat taatttcgct cactggttgc
     5941 ctcctggaaa ataaccgcat gtcccagttt ctccgccagt gccagttctg ccttagcgcc
     6001 tgccgatcgc tgccagcctt tcagcatata aaccgcatcc acacaacgaa tcattgccat
     6061 gcaaatatcc atgtactgcg gctgagtcag cccgtccgga agtactgccg ggtttaaaac
     6121 ggtatgccct tcccgtttca gtgcttcttc cgccctgtga aacgcctcgc ggttgaaatt
     6181 ttcatatccc gtcattggac cggcgatata aatgctcacc ctcacgcctg aaccctcctg
     6241 tcgaaataaa cgtagttatt cactatgcgc agcggcatgc ctaattttct ggcaatttcc
     6301 cttctttgca tgcctctctg atgcagttgc cgcgccagct caatatcacg ctgagaatat
     6361 tttgccgacg ggtgaaaatc accacgtaac atcatgctga tgcccaactc ccgtgccttc
     6421 gttctcactg ccgcttcagt tcgtccgata agtgcgccaa tgctttttac cttcattgtt
     6481 cccgcgcact gccggagtat cagaatttca gcccggcacc acgtcttcca cccactcacc
     6541 gctgctgttc tctggtggcg gtaatatccc agagaatatc ccggcacttg ttcagctccc
     6601 gcagcgcggc gcagactcgc tcccacttct gaacctgacc ttttgcccgg cgcagctcgc
     6661 ggttagccac atgcagcgat ggtagaatca ggtcatctgc tttcgtttcg gtgaccgatg
     6721 gctgtaactt cacaatgtct tccacgattt ctgttttcat ttcttcctgt gtcgtcgttt
     6781 cctggactgg taacgcaaca cctgctggct gaggaaaggc tttaccatcg gtttccgcta
     6841 cggatgcagc ttccggctct gccggtaaat cagcgcccgg tatgcagtaa cgaaatttac
     6901 cgccctgatt tacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     6961 cccggcggga ggtaatcccg aacataagtg ccagctcatc cgccgtttgt gggccatgtt
     7021 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     7081 tttcacctgc ctgagtcagc caccacatcg accccttgtt atccgcttca ccacggcgct
     7141 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gccgccacta
     7201 cctgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacggtttcc attaaaattt
     7261 cctccgacaa aatcgtttcc cagattcaaa taaaaccagc agccttccgg cgttcgtatt
     7321 cctgtttcag ccgttcaatt ggcgttggcc ctttcgggtg tttcgcccct tccagttgtc
     7381 gtcgcactgg cggaacactc atcccgttac caacatgctt tgcccatttc gtcagttgcc
     7441 gttccgcaag tcgttttaac tcaccctgcg tcatctggcg ctcaatccct ctggtacgca
     7501 tttcgaggca gatgtggtac agcacaggct gtggccacgg gtatttatca ctcccgtcgt
     7561 atcgccagga ttcattgcgc cagcgccggt actcttccat cacggcatcc accgtaagac
     7621 caaatggatt tgccccactc tccgaaatca gcgcaacaaa ctcagccagg tccgggggcc
     7681 acgtttcacc cgcccggcag cggtccatgc actgacggca gaccagacgg atttgctgtt
     7741 cagtcatcgc accaatctgg gcaatccaga gcttcgaagg tgcggcccca ttcttctgag
     7801 tccagcggtt cgaatacacc tcccccataa gctcccacag cttccaggcc gtttccgttg
     7861 ctgataaatc cgttttcacg ttcccactgt tcgcgtgctg cccggatttc ctgaactgcc
     7921 cgtgatgccg tgccacctga tgctgcatgg cttaccccct tgctgactgg ttttacctgt
     7981 gccctgacgt gctgcacgtg acgggcaaat ttctgctccc actgaacctg cgtgaaaacc
     8041 ttcccctccg ccatccagta atcccggaat gcggcaagct ctgcaggtgt aaattccggc
     8101 tcaggcagag ccatacccca cactactgcc cgttgtcgaa aatccagcga cggctgccag
     8161 acagtagtca tcgaaaattt cccgatgggt tcgctcaggc catccaagaa tacagggggc
     8221 gctgcctgta acgacaaaac ttcctgctca ctggtcggag cactctcgcg tgcgttatgt
     8281 gtggggttta gatctttggg ttcctttggg ttccgtgatc cgtttttggg tgtctttgat
     8341 ggaaaatttg ggtgtctttg gttattttcc atgcagctaa gagttccgtt tttgggtctg
     8401 ttttgtgctg aaacataacc attttcggta ctatttttat taacagtacc aattttaccc
     8461 acctttaaag actcccgttt ttgggtgtat tcaggctcgg caacactttc ttctacaccg
     8521 ataagtcggt acaccacaat ttgctttgtt ctgccttttc tctcaccagt atcaacaatt
     8581 aacccaatct ccatcaggtg tcgtaaactg tcctgcacag tctttttgtt cagttccgtt
     8641 acttctgcca gtgcagatat agacgggtat gcgcacaaat cggcaccgca catatcagca
     8701 agccaggtca atactgactt actggatgaa ctgccggttt tcaccttttt agcccatcgt
     8761 agtgcatcga tactcatacg aaccccagac agatatttgt ttatctgcaa agtaatgttg
     8821 gtattgctga cgatacgcac gcttgaaagc aatggctttt tctataagct catcagtctc
     8881 acgttccaca acaactggat ccgcaaaaag cagcccggac tccaccacat cgccatactc
     8941 tttgtttaac ccggcgatca tgtacgtaat gctttttccg tcagtaattt cacaatacaa
     9001 cctgaaatca cttacccgga tagcctccat aattgccgga atcagcgccg tgaatttttc
     9061 acgcttatcc ctggtgtcga tagccttcca gcgttcgaat atcttcactc gattaacgcc
     9121 cagcgcccgt tgatcaacct cgccatcatt aaacgtgacg cgttgaacat cgatgttcgg
     9181 gcgttctttc agagcccaga atgcttccgt gattaatatc gtcgcctgct cctgtgtcat
     9241 tcctggtcgg catacccagg catccagagc ctcacaaacc tgttcagggg tgattttcat
     9301 tgttcaccgc cagtgattca ttcgctgtag gcttaatcct gccaatagtc aaaaccgcat
     9361 cagcagaaaa taatccgttt gatgccagag caattttttc agcgtaattt gtttcgcctg
     9421 tatattctgt gcgcggtaat tttccattat tcatccattt gtagattgct ctttggctga
     9481 caccgcaaac atcggctaca acagaaacgc gaactgcttt gattacatct gcaagtgttg
     9541 tatggctcat atcaccctca caatgtgaac tttgagtaca caatataaca gaactgacag
     9601 tacattcaat agcaaatatc attgaactta tggttcatga agatgaagcg cgtaaagagt
     9661 tcgccagcag gcttgcgcta gcctgtgaaa atgctggctt tgaacaacac ggaaggcagg
     9721 cagaaattgc ccgtcgaatg aagctaacac ccaaagcggt tagtaaatgg tttaatggag
     9781 aggcaattcc ccgtcgagag aaactgaaag aattggcagc acttatcgga acaacaccaa
     9841 cttaccttct gggggaggac actgaagaaa gcggaagcct gcgtttctat caggagttaa
     9901 gtccgaagca aaaaatcatc attgaacttc ttgatgaact ccctgacagt gaaactgatg
     9961 aacttttaaa aactcttgag gagaaaaaac agaagtacaa cgcaatttat gaagaattag
    10021 cgcgaaagaa aaaacaaaaa gcctcctgaa tctgcataaa tccggtagct tccctccggg
    10081 tttatgctcc tctcatcccc tcctgatatt ttacccctca caaaaatgta ctaaaagtac
    10141 tttacattaa tgaacagcag gtacattata tcaccaaccc accccgcccc acagaacgcc
    10201 aggcaatact tcgagttacc aggcagtggt caggggttaa gtagccagcc cgaggcgtat
    10261 gaacatgacg gcgggaacac tttgtataac agcgcagcag gtttttagtt ccgcgacccg
    10321 gcgttaaggg caaatgaggt caacatggat acgctcaatc ttggcaacaa cgaatctctg
    10381 gtatgcggag tattccccaa ccaggacggc acgtttaccg cgatgacgta taccagaagc
    10441 aaaacgttta aaactgaagc tggcgcgcgt cgctggttag caagaaactc tgactaatga
    10501 agtctggtag ttaaggaatc ctccacgggg agaactggtg cacacgcgcc ggacacaagc
    10561 aagcatccgg catgctcttt aacaatctgg atatccataa cagtaacaat ctacagattg
    10621 ccgttcagtt ttctggccaa ctcctcaata gatggaggcg atacataatc cggattttta
    10681 ttcataagaa actgattttc acagtagagg cacctgcttt tatgaaaaaa ctcatgttct
    10741 ctaaccggga atggttgaag tatcgatact atcttttgtc caaaacattt tggacaaaga
    10801 tgcatgatta tgctgccgcc gttcacggtt acctccttcg agtacacaaa agttcccgac
    10861 tcaagttggt taaggatata accttccgtc tgagcctcaa agttttcaaa ttctgcaatt
    10921 ttagctttga gagaagcatt tatttcttga taagtgctca ccagctcaac gagagacacg
    10981 cattcgtgct gaatagatgc aagctttgag ttcagttcac caatagccgc atttacttca
    11041 gcttgagttt ttgcctcgtt cattagtttt gtaatctgag cggtttcacg aatagccgtc
    11101 attgctgccg ttaattcagc gatcacattc aatactctta ttgttgttgg ggatatccag
    11161 attaacaagc tccttgttgt tggggaataa caggtccacc tcgcctgacg tggttaaaag
    11221 caggcacaca acacgaaagc gctcggcgaa gttagtctct ctgtatatgt cgtcgttaaa
    11281 tgtaattcga tcgtgcgctt ccggttgtgg caatccgcga aatggcgcgg cggtaagtat
    11341 ggctggggct tcctccattg ctccagaaaa tgcaccgggt tgtcaggttg accatacgcc
    11401 tgagtgacaa caccgccaca acaacctctg ttatcacttt tctggtgatt cggcggaaat
    11461 ggatatccgc catttttaaa gtgtattttg tgatgcggtg aatgcggcta agcgcacgcg
    11521 gaacagttaa aacaagcggt cttttactgg cgtaacagac atcaactaac aatccggcgt
    11581 taattgttaa ctggttaacg tcacctggag gcaccaggca ccacatcaca aaattcattg
    11641 ttgaggacgc gataatggaa acgtcactac caaacgttaa tacgtctgaa gggtgtttta
    11701 atattggtat tctgctcagt aaccgggagt ttactgagga cgccatcagg atgagaaaat
    11761 atgagcctta tcttctcaat gataattcca tactctccag aattgccctt cttgaacttg
    11821 gcattttcgg agggcagcag tgacttctgc atttgcactg gtaatgacgg tttttcttat
    11881 aacgggtgag ccacagaatg tgattaccgg aatttatgcc agtaaagaat cctgccatca
    11941 ggcaagagac gagcaaaaaa tttccggtga atgtctcccg ttaaacaaag tatcgctgta
    12001 cctgaataac gaaataccgg ctggataacc cgccagccat attaacgcca taccaacgga
    12061 ttaaaaatgc cagcaatggc agggattcgt tcaccctgaa atctgtaatg aggtttaaac
    12121 acaatgagta aaatatttat ttgcgccgcc attcctgacg aacaggcaat aaaaaacgag
    12181 ggcgctgttg ctgtggccac agccattgaa gccggtgatg aacgtcgcgc ccgcgcaaaa
    12241 tttcactggc aattccttga acattatccg gctgctcagg actgcgctta taaatttctt
    12301 gtctgtgagg ataaacccgg tacaccccgc ccagccctcg actcctggga tgctgaatat
    12361 atgctggaaa accgctggga tgaggcgtct gcttcctttg tcccggttga gactgaatca
    12421 gatccgatga acgtcacttt tgacaagctg tcccctgaag tacagaacgc tgtcatggtt
    12481 aagttcgaca catgtgaaaa catcaccgtt gatatggtga ttagcgcaca ggaattgttg
    12541 caggaagaca tggcaacctt cgacggacat atcgttgaag cgttgatgaa aatgccagaa
    12601 gttaacgcca tgtatccgga gattaaactg catgccatcg ggtgggttaa gcataaatgt
    12661 aagcctggtg ccaaatggcc agaaattcag gcagagatgc gcatctggaa aaaacgtcgc
    12721 gaaggtgaac gcaagaaaac cggaaaatat acgtctgttg ttgatctcgc acgcaccaga
    12781 gttaaccagc acctcaccga aaactcagca gcaaaaatca accctgtcac tacagccatt
    12841 cgtcgcgaat acaggcagac atggaaaaca ctggataaag aactggcctg cgccctctgg
    12901 cccggggatg tggatacagg aaacattgac ggtaccatcc atcgctgggc taaaaatgag
    12961 gttatcgaca aggatcgcga agactggaag cgtatctcag catcaatgcg caaacagcct
    13021 gatgcgcttc gctatgaccg tcagactatt ttcggccttg ttcgtgagcg cccgatcgac
    13081 attcacaaag atcccgttgc actgaacaaa tacatcagcg aatacctgac gacaaagggc
    13141 gtgtttgaac atgaagaaac agaccagagc tctgctgatg ctatcctgtc gtcagcagca
    13201 caaactgatc cagtggagac ggcggaatcc aattctcaaa aaaatgaaat cctggtggaa
    13261 gctgaaccat ctgtagagcg tgaaggaccg ttttatttcg tctttaccga taaggacggg
    13321 gaaaaatacg gcagggcaaa caaactttct ggcctggaca aggcgctggc tgccggtggt
    13381 accgaaatct caaaagaaga atattttgcc cgaaaaaatg gcacatatac gggcttacag
    13441 caaaatacag ataccgcaga agattcagaa caaccagagc cggtaaaagt taccgctgac
    13501 gaagtaaaca aaattatgca ggcagccaat atcagccagc ctgacaccga taaattgctt
    13561 gctgcatcac gtggtgaatt tgttgaaggg attagcgacc cgaatgatcc gaaatgggtt
    13621 aaggggattg aaacccgcga ttctgtgaac cagaaccagc ccgaatcgga acaaaacgac
    13681 cagaaagcgg aacaaaacag tccaaatgcg ttacaaaacg agccagaaac gaaactgcct
    13741 gaaccagaag tgcaacaaga accggaaaaa gtctgcaccg cctgcggtca gaccggcgac
    13801 ggcaactgtc ctgactgtgg cgcggtaatg ggcgacgcaa cataccagga aacattcgat
    13861 gaaaaaaatc aggctgaagt tcaggaagat gattcggagg aaatggaagg cgctgaacat
    13921 ccgaacaatg agaatgctgg taacgatcag catcacacca gcgatagtga aactggcgag
    13981 gcagcagagc ccttgattgc ggtgaatggt catcacatta tcacatccac cagcagagtg
    14041 tggattcacc tgtcggtcga ccttgaaacg atgggaacaa atcctgatgc gccaatcaac
    14101 tctattggcg gtaaattttt tgatccggca accggagaga tggggcctga attcagtaaa
    14161 gctatcgatc tggaaacctc cggcggaatc atcgaccgta aaacaataaa gtggtgggcg
    14221 aaacgatcac gcgaagcaca atccgctatt tttaccgatg aaatctcgtt agatgttgct
    14281 ctccggctat tcatagaatt tatcgaaaaa aactccggcg ggcgttttgt ccaggtatgg
    14341 ggaaatggag ccaacttcga caacgtaatt ttacgccgtt catatgaacg gcaggggatc
    14401 ccctgcccgt ggctttacta caacgatcgc gatgtacgca caatcgttga actgggtaat
    14461 gccattggct ttgatgtcag aatggctatt ccattcgaag gcgtacccca caatgcgctg
    14521 gacgatgccc gtcaccaggc aaaacaagtt tcagcaatct ggcaaaaact gatcccgagt
    14581 caggctgatt tttaatgttc aaccccggtc gtcgtccgca agctatagtg gcggcggcca
    14641 tgattagcga acaacgctca tggcaagact tattctgctc actgagtggg caaaagagga
    14701 attcagtgaa ccggtcccta ctccgggcac gttaagtaaa tacgctaaag ccggaatgat
    14761 atttcctctc cccaaaaaag ttggaagaca ctggcgagtg gatcctcgag ctcgctttgt
    14821 cggaatggta aacaagccgg aagtgatcgc cacagatcac cctgctttga agaggatact
    14881 ggaagatggc gcgcccacga aaatataaaa ctgaagttcc gggattatct ccgtattttg
    14941 acaaaagaaa taacaaagtt tactggcgtt acaggcatcc cataacagga aaaaatcacg
    15001 ggctcggcag tattgaccag aaacaggcag aagctattgc agcagaagcg aacagccgtc
    15061 ttgccaggca acaaatggaa caaatgctca ctctgcagga gaaaattatt cgtgataccg
    15121 gtggttcatc aaccgtttct gtttttctga atagttaccg aaaaattcaa caggaaagat
    15181 atgaaaacgg agagatcaaa ctcaacacac tgaaacagaa agcatcccct ctcagggtat
    15241 ttgatgaacg ttttggtacc agacctttag atgccataac cgtaaaagat gtggtatcgg
    15301 tgctggaaga ttacaaggca aaaggacata acagaatggg acaaattttc aggaaagtac
    15361 tgattgatgt tttccgggag gcccagcaaa cgggcgatgt cccgccaggc tttaaccctg
    15421 cagaatcggc aaaaaaacca caggtacgga tatcacggca acgactgacc tttgatgagt
    15481 ggatgataat ttataatgca gcggaaaagg atggttactt tttacagcgt ggtatgctgc
    15541 tggcactgat gacaggccag cgcctttcag atatttgcaa aatgaaattt tcggatatca
    15601 gggatggtta tcttcatgtc gaacagcaaa aaacaggaac ccggattgcc atccctctgg
    15661 ctctgcgttg cgataaatta aatctcaccc tggatgatgt ggtgtcatcc tgtcgcgatt
    15721 gcgttcttag tccgtggcta ttgcaccacc atcatgctaa agggacagct aagcgcggcg
    15781 ggatggttaa gccagcaacg ttaaccgttg catttaaaaa agcccgggat tcagtggatt
    15841 acaactggcg tgctaatggc accccaccct ctttccatga gcagagatct ttatcagaac
    15901 gattgttcag agaacaggga attgatacca aaattttgct gggccattcg aatcaaaaaa
    15961 tgaccgatat ttacaacgac gcacgcggta aggaatggaa aaaactggtc atttgatgac
    16021 cagttttgca gaagagtttt gcagaggttt tgcagagcaa attaaaaacg gtaccctgca
    16081 gagaacataa acacccacgg atcaaggcgt acattgtcgc tataactgcc tttagccagt
    16141 ttacgaccat cagatttata cttagccgtg gtatcgatat ccatgtacca cactgacatg
    16201 ttaaccaacc agtcacggtt aatcagataa tcaaccccca cctgcccggc agctccccag
    16261 gaatctttca gactgagatc ggaaagcccc gcctctttgc catgatcgtt aaatccatta
    16321 tcaaagaagg tggtgtagtt aataccggcc ccaacgtaag gacggaattt gctgctggca
    16381 tcaccaaaat accactgcgc catcagtgtt ggcggcagat ggtgaacggt tgcaatatcg
    16441 ccggtcgccc gggtgccgat tttatggcgg aacggcgtcg ctgccagtaa ttccacacca
    16501 atgttgtcgg tcgccatata agtaaacgtc aagcccagtt gagtgttatt ggtcacgctg
    16561 aatccaccca gacttcctaa cgtaccacca gcaccttctg ttggacgtac ggttgcagaa
    16621 cctgcacgca taaaaaattc gcctgcttca tgcgcaaagg cactgccaga aagaagagtt
    16681 gttactgcca aagccgccac tgttaacttt ttcatatccg ctccgtcgtt atggttataa
    16741 aaacgtgacg aatataccta cattggagta acaaatgatc ctacatagat cacattacat
    16801 atgataattc accatttatt gatctggatt aatttagaag ttgcattgag aatcctggtt
    16861 aatgtgattt aaatcaattt ttaaatattt tctctacgca aatattaact cgatcgccac
    16921 ttgccgcggt ttgcccacgc ctgaaggcca gcaattacct ccgctttaca aagatatgct
    16981 tttccataca agacctgatg ctgccacccg aggcttaacc agggtacaat tgcccgctaa
    17041 ttaacacctg cataaaactc aaggagagtg catgtctatc acggcgcagt ccgtataccg
    17101 tgacaccgga aatttcttcc gtaatcaatt tatgaccatt ctgttggtat cgttgctatg
    17161 tgcgtttatc acagtggtgt tagggcatgt tttctcaccc agtgatgcac agcttgcgca
    17221 gctcaatgac ggcgtgcccg ttagcggcag tagtgggttg ttcgacctgg ttcagaatat
    17281 gtcaccggaa cagcaacaaa ttttgctgca ggcttcagcg gcgtccactt tttcaggatt
    17341 aatcggtaac gccattctcg ccggaggcgt aatattaatt atccagctgg tgtctgcggg
    17401 tcagagagtc agtgcgctgc gagctattgg tgccagtgcg ccgatattgc ccaagttatt
    17461 tattctgatt tttctgacta cccttttagt acagattggc atcatgctgg tggtcgttcc
    17521 gggaattatc atggccattt tactggccct ggcaccggtg atgctggtac aggacaaaat
    17581 gggcattttt gcctcgatgc gtagcagtat gcggctgact tgggcgaata tgcgtctggt
    17641 ggcacccgca gtactgagct ggttgctggc aaaaacactg ttgctgcttt ttgcctcttc
    17701 ttttgccgca ttaaccccgg aaattggtgc cgtactggcg aacaccttga gcaacctgat
    17761 ttctgccata ttgctcatct atctgttccg cctgtatatg ttgattcgcc aataacctta
    17821 agcatttgat tacggaatcg taaaatgaag cagtttcttg attttttacc gctggttgtc
    17881 tttttcgcgt tttacaagat ttatgacatc tacgcggcta ctgcggcgct gatcgtcgcc
    17941 acggcgattg tgcttatcta tagctgggtt cgctttcgta aggttgagaa gatggccctg
    18001 atcacttttg ttctggtggt cgtcttcggt ggcttgacgc tgttcttcca caatgatgag
    18061 tttattaaat ggaaggttac agtcatttat gctctgtttg cgggagccct gttagtcagc
    18121 caatgggtga tgaaaaagcc gctaattcag cggatgctgg gtaaagaact cacgctgccg
    18181 caaccggtat ggtcgaagct aaatctggct tgggctgttt tctttatcct ttgcggtctg
    18241 gcaaacatct acatcgcatt ctggctgccg caaaatattt gggtcaactt taaagtcttt
    18301 ggcctgaccg cccttacctt aatcttcaca ttgttaagcg gtatctatat ctaccgccac
    18361 atgccgcagg aagataaatc ctaaccagac tgccagtcct tcggggctgg cgctgtatct
    18421 cctctgttct cctcaggaaa atcatagtag catcgcgcct gtgattttcc ttttaagtcg
    18481 gttttaccat gtctacaaca cataacgtcc ctcagggcga tcttgtttta cgtactttag
    18541 ccatgcccgc cgataccaat gccaatggtg acatctttgg tggttggtta atgtcacaaa
    18601 tggatattgg cggcgctatt ctggcgaaag aaattgccca cggtcgcgta gtgactgtgc
    18661 gggttgaagg aatgactttc ttacggccgg ttgcggtcgg cgatgtggtg tgctgctatg
    18721 cacgctgtgt ccagaaaggg acgacatcgg tcagcattaa tattgaagtg tgggtgaaaa
    18781 aagtagcgtc tgaaccaatc gggcaacgct ataaagcgac agaagcatta tttaagtatg
    18841 tcgcggttga tcctgaagga aaacctcgcg ccttacctgt tgagtaatag tcaaaagcct
    18901 ccggtcggag gcttttgact ttctgcttac tgaatttcgg tggtgccgtt aattttaaac
    18961 aggatattca ccacaatccc actgcctggc ttacccggct cataacgcca tctgcgcatc
    19021 gcatttttca cctcacgctc aaacatgttc gcaggcttgg ctgagaggat ttgtacgtta
    19081 tccacgcgac catccggcgt gacgtcaaat ttaactttaa cctgcccttc aatgcgcaat
    19141 gcctgtgctc gtgccggata ctgcggctga ttacggctta atgcgcgtgg tcctgaagcc
    19201 acactggtaa ccggcttgct ggttgcagcc gttgccgtac ttgatgtcag gcgcgcgggt
    19261 gccgtatttt caaacggtga tgccggacgc gactctacgg gtttgatatc gcgttttggc
    19321 tgctcctgta cctttttcac cggcttcggt tttggcttag gtttcggctt cggcttttca
    19381 atgaccaccg gtgcttcttt tggcggttcg gggatcggct caggttccgg ttctggctct
    19441 accaccggct ccggtggcgg ctgaacggct tgtggcggtt cgagatcagc aggcgcaacc
    19501 atcgtgacag aaattggctg cgcaggcgca ggtagttcaa taacctgatg taccgaggta
    19561 tagagcagac ccgccacaac agcaccatga atgcagaccg aaagtaacgt cggccagggg
    19621 aagcggcgag gtaaatcaag ggtcattgaa gtcataatca tttcagtaga aaaaccaggt
    19681 ctcgatttta aatgcaaata gcaatcatat tcaataaggc aagacgatca ggatcagtga
    19741 aaatgtcatt tttggcgtaa ttgaccttac tttatgcatg gtcttaacaa agacatcatc
    19801 tttccgttgc agatcttcgc cctttcaaat aacgtacttt acaactttcc cgaacaagga
    19861 gttgtgcccg tgttgtatgt tatttacgct caagacaaag ctgatagcct cgaaaaacgc
    19921 ctttccgttc gtccggcaca tttagcacgt ttacagttac tgcatgatga aggtcgtttg
    19981 ttgacagctg gtccaatgcc agcggtagac agtaacgatc caggggctgc gggatttact
    20041 ggctcaaccg ttattgctga atttgaatcg ctggaagcag ctcaggcctg ggccgatgct
    20101 gacccgtacg tagcggcagg tgtctatgaa cacgtttcag tgaaaccatt taagaaagtg
    20161 ttctgaatag agattcaata tttaagggca cggtttttgc aaccgtgttc ttgtttggcc
    20221 tttccttata gccggtcagt gcattcacta ccacaagcag ataataactt tatctgtatt
    20281 tctttatatg atgtgaacat attcaggaaa atgccctttc ctgacgcaga gttgctcgct
    20341 caaaaccttt gattactgcc gataaagtca tctactattg tttaacacat tcacgcgctg
    20401 catgtatgtt cagaaaatgt gcacgctaag ataattaaac aaaattgtgg ttacagaggt
    20461 atcagtgagt cactgggcta cattcaaaca aacagcaaca aatttatggg tgaccctacg
    20521 gcacgacatt ctcgcgctgg ccgtcttttt aaatggattg cttattttta aaacaatcta
    20581 tggtatgtcg gtcaatttgc ttgatatttt ccatatcaaa gcattttcag agttggatct
    20641 ctccttgctg gcaaacgccc cactatttat gctcggcgtc tttcttgtcc tgaactccat
    20701 tggcttactg ttccgggcaa agctcgcatg ggcaatcagt atcattttgt tgttgatagc
    20761 gctaatttac accctgcatt tttatccctg gctgaaattt agtattggat tttgcatttt
    20821 tacgctggtg tttttgctga tactgcgcaa agacttctcc cacagtagcg ccgcagccgg
    20881 gacaattttt gcatttatta gtttcacgac attactgttt tactcaacct acggtgcgct
    20941 ttatttaagc gaaggtttta atccgcgaat agaaagtttg atgaccgcgt tctatttttc
    21001 gatagaaacc atgtcaaccg tcggctatgg cgatattgtc cctgtttctg aatcagcacg
    21061 attgttcact atttcggtca ttatttccgg cattaccgtt tttgccacat ctatgacctc
    21121 aatttttggt ccgcttatcc gcgggggatt caacaaactt gtaaaaggaa acaatcatac
    21181 aatgcatcgt aaagatcatt ttattgtttg cggacattcg attctcgcca tcaatacgat
    21241 cctgcaactg aatcaacgcg gacaaaacgt aacggttatc agcaacttgc ctgaagatga
    21301 tatcaagcaa cttgagcaac gcttaggcga taacgctgat gttatccccg gtgacagtaa
    21361 tgacagttca gtattaaaga aagcgggaat cgatcgttgc cgggccattc tggcgctgag
    21421 tgataacgat gcagataacg cgtttgttgt actctcggca aaagatatga gcagtgatgt
    21481 caaaacagtt ctcgccgtca gtgatagcaa aaacctgaat aagattaaga tggtacatcc
    21541 ggatatcatt ctctcaccgc aactgtttgg cagcgaaatt ctggcgcgag ttttaaatgg
    21601 tgaagagatt aataatgata tgctcgtttc aatgttgttg aactccggtc atggcatttt
    21661 cagcgataac gatgaacaag aaacgaaagc tgacagtaaa gaatcagtgc aaaaatagag
    21721 tttaaagacg aaagagtaaa cttattgggc accgcaatgg tgcccagtat atttagatat
    21781 tgtagatcgc aaacaataac gagttacgct gtttgtttag aatgcacttc ctgatgctgt
    21841 ggatacgcat atttcggcgc gattgcccct caagccaacg cgatttacgt cggctagcct
    21901 gacgctgcat gcgccagcgc cccacttccg ttctactccg cttcatgtta actactctat
    21961 gcaataacag aatggtcatt atacccccac ccccgacgct gaccagtgct tttcccgcgt
    22021 ttttatttgc cagatgaatc catatgcgta aactcataac aatgcgcttt caaaaggatt
    22081 tctaatctat gacaaccgtt tatacgttgg tgagttggtt ggccattctg ggatactggt
    22141 tgctcattgc aggcgtaact ttacgcattc taatgaaacg acgcgcagtt ccctccgcga
    22201 tggcctggct gttgattatt tacattctgc cgttagtcgg aattattgcc tatcttgccg
    22261 ttggcgagct ccatttaggc aaacgccgcg ctgagcgcgc cagagcgatg tggccttcca
    22321 ccgcaaaatg gcttaacgac cttaaagcct gtaagcatat cttcgccgaa gaaaatagca
    22381 gtgtcgctgc gccattattc aagctttgcg agcgtcgtca ggggatcgct ggggtcaaag
    22441 ggaatcagct acaactgatg accgagtcag atgatgtgat gcaggcgtta atccgcgaca
    22501 tccagctcgc gcgccataat attgagatgg tgttttatat ctggcagccc ggcggcatgg
    22561 cggaccaggt ggctgaatca ttaatggcgg ctgcacggcg tggtattcat tgccgattga
    22621 tgctcgactc cgccgggagt gtggcttttt tccgcagccc gtggcccgag ctaatgcgta
    22681 atgccggtat tgaagtggtc gaagccttaa aggtcaatct gatgcgtgtg tttttacgcc
    22741 gtatggacct gcgccaacat cgcaagatga tcatgatcga taattacatc gcgtacaccg
    22801 gcagcatgaa tatggtcgat cctcgctact tcaaacaaga tgcgggcgta gggcaatgga
    22861 ttgatctgat ggcgcgtatg gaaggcccca tcgccaccgc gatggggatt atttattcct
    22921 gcgactggga gattgaaacc ggaaaacgta ttctgccgcc accaccagat gtcaatatta
    22981 tgccgtttga acaggccagc ggtcacacca ttcacacaat tgcttctgac cccggctttc
    23041 cggaagatct cattcaccag gcattattga ctgcggctta ttcggcgcgt gaatatttga
    23101 tcatgaccac gccctacttt gtgccaagcg atgatttact tcatgcgatt tgcacggcgg
    23161 cgcagcgcgg ggtggatgtc agtattatcc ttccgcgaaa aaatgactcg atgctggtcg
    23221 gctgggccag tcgcgcattc tttacggaac tgcttgctgc tggggttaaa atttatcagt
    23281 ttgaaggcgg gttactgcat accaagagcg tgctggtcga tggcgaacta agtctggttg
    23341 gcaccgttaa ccttgatatg cggagtctgt ggctgaattt cgagattacc ctggcaatcg
    23401 acgataaagg ttttggtgca gaccttgccg ccgttcagga cgattatatt tcgcgttcac
    23461 gtctgctcga tgcccgttta tggctaaaac gtccattatg gcaacgtgtc gccgagcgac
    23521 tgttttactt cttcagtccg ttgctgtaaa acgtcgccaa cagaggttaa acaggtagtg
    23581 actatggata tggatctaaa caatcgcctg actgaagatg aaacgcttga gcaggcctac
    23641 gatatttttc tcgaactggc tgccgacaat ctcgatccag ctgatgttct gctgttcaat
    23701 cttcagtttg aagagcgcgg cggcgcggag ttattcgatc cggcggaaga ctggcaggaa
    23761 catgttgatt tcgatttgaa cccagacttt tttgccgaag tggtgattgg tctggcggac
    23821 agtgaagatg gtgaaatcaa cgatgttttc gcccgcattc tgttatgccg cgaaaaagat
    23881 cacaaacttt gccatatcat ctggcgggaa taaataatcg ctttcggcgt gaaaaagggc
    23941 tgacaactgt cagcccttat tgtgtttaaa gcggatcgac tttcaggcaa gaaacggcgt
    24001 gtctgaaact cccctccaga acaggacgtg ttttggcgca ctccggaccg gcaatcgggc
    24061 aacgggtacg gaaaacacaa ccggaaggcg ggttgatcgg cgacggtaat tccccttcca
    24121 gtaactggat ggttttgttc ttctccagat ccggatcagg tatggggact gccgacatca
    24181 atgccctggt gtaaggatgt agtggattgt ggtagacctc atcataggtc cccagttcta
    24241 ccgcatggcc gagatacatc accaacacac gatcggaaat gtgttttacc acggccaggt
    24301 catgagcgat aaaaattaat gacaatccca tctcacgttg cagctgctgg agcaggttga
    24361 ccacctgcgc ctgaattgac acgtccagcg ccgaaaccgg ctcatcgcag ataatcagct
    24421 tcggttcaag aataagagca cgagcaatcc cgatacgctg gcactgccca ccggagaact
    24481 catgcggata gcggttaatc aggttaggca ataacccgac tttcagcatc atcgccttca
    24541 cgcgctcgcg aacttcctgg cgtgacattt tcggatgata agtacgcagt ggttcagcga
    24601 tgatctcgcc gatggtcata cgcgggttta gcgatgccaa cggatcctgg aaaatcatct
    24661 gaatatcact gcgaacggca cgccattcat cgggcttcat gcccagcaac tctttaccta
    24721 accaggcaac atgaccgtcg gtcgccttga ccaaaccgat gatggcgcga gcaaaggtgg
    24781 acttaccgca tcccgattcc cctaccacac ctaatgtttc cccttcatac aggcgaagag
    24841 tgataccatc gacggctttg agcgttttcg gcggttgcca gaaccactgt ttgccatctt
    24901 tgatttcaaa gtgcacttta agatcggcga tttcaaggag gacttttctt ccttcagtta
    24961 cagcattcat aacagttcct ccaccggttt aaagcaagca cgcagacggc caggcgtaaa
    25021 ctcttccagc ggcggtgcgc tactacaaat ttccatcgca tgtggacaac gtggctggaa
    25081 cgggcaacct ttcggtaatc gcagcaagtt tggcggatta cccgggatgg tcaacattgt
    25141 ttcaccttcc gcatcaagac gcggcaccgc gttgagcaaa ccgatagaat aaggatgaac
    25201 gggttgataa aagacatcgc gcgcattgcc atattccatc gtgcgcccgg cgtacattac
    25261 cagcactttg tcacagatcc ccgccaccac gccaagatcg tgggtgatca taatgatggc
    25321 ggtattaaat tcccgcttca gttcatttaa tagcgtcatg atctgcgcct gtacggtgac
    25381 gtccagcgca gtagtgggtt catccgcaat cagtagctta ggtcgacata gcaatgccat
    25441 cgcaatcatg actcgctgac gcatgccgcc agaaaattcg tgcgggtaca ttttcatgcg
    25501 tttacgcgct tccggcattt ttaccgcatc gagcatccgc accgactctt caaacgcctc
    25561 agctttgctc atgttcttat gcagcatcag cacttccatt aactgctcac cgacgcgcat
    25621 atagggattc aacgaagtca ttgggtcctg aaaaatcatt gagatttgtt cagcgcgcag
    25681 tttattgagt tcatgctctg gcaaattgag gatttcacgc ccattgaagg ttgccgatcc
    25741 gccaatacgt ccgttggcag ccaacaggcc catcaacgca aatgcagttt gcgatttacc
    25801 cgaaccagac tcacctacaa tacccagcgt ttctccggca cgtagggaaa aattcaaatc
    25861 attgaccgcc gtgacgtcgc cgtccggggt actaaaggtg acacgcaaat ctttcacgtt
    25921 cagcagtgcg tcagcctgtt gttgtgcgag cggcacagtt gcagtttcaa ttacgctcat
    25981 ggctgcactc cttaacgatc tttcgggtcg agggcatcac gcaagccatc gccgataaag
    26041 ttgaaacaaa acagcgtcac cacgaggaat cccgctggga acaacaataa ccatggagag
    26101 acttccatcg agttcgcgcc atcactcagc aatgcacccc agctgcttaa cggctcttgc
    26161 gtacccaacc ccaggaagct aaggaaagat tcaaagagga tcatgctggg caccagaagt
    26221 gatgcgtaga ccaccaccac accgagtaca ttcggcacaa tgtggcgaat aacaatgccc
    26281 gatgtcgata caccgccaac ttgtgccgcc tcaataaact ctttgcgctt cagactcagg
    26341 gtttgcccac gcacaatacg agccatatcc agccaggaaa ccatgcctat cgccacgaaa
    26401 atcagcagga tgttttgacc gaaaaaggtc accagcaaaa tgacgaagaa catgaatggg
    26461 aaggagttga ggatttccag cagacgcatc attaccgaat ccactttacc gcccagataa
    26521 ccggaaagcg aaccataaag tgtcccgacg accacagcca ccagtgccgc agcaacaccg
    26581 accatgagtg agatacgccc gccaatcgca acgcgcacaa gcaggtcgcg accggatgag
    26641 tcagtaccaa agtagtgacc ggactccata tccggggcgc tggacatcat cgcccagtca
    26701 gtatcgtcat aggcaaactg ggaaagcatc ggtgccagga ttacaaataa cgcgatcagc
    26761 accagcacta tcagactggc aaccgccgca cggttatgaa taaaacgtcg acgtgcgtcc
    26821 tgccacaagc tgcgcccttc gacctccagc ttttcactga aattttccag cgtctcgctg
    26881 tttttcttac ttaacatcat cgcgagctcc agtatcagta acggattttc gggtcgataa
    26941 ccgcatatag cacatcgaca atggcattaa acaaaatggt taaagcacca accaggatgg
    27001 tcaggcttaa cactaaggaa tagtcacggt tcaatgcgcc attaacgaac agttgcccaa
    27061 tccccggcaa accataaatg gtttcgatga ccatagaacc ggtaataatg ccgacaaatg
    27121 cggggcccat ataggagagc acgggtaaca gagcaggttt taatgcgtgg cgtaaaatga
    27181 tccgccgcat aggtaaccct ttcgcccggg cagtacgaat aaagttggag tgtaatactt
    27241 caatcataga gccacgggta atacgcgcaa tactggcgat ataagcgagt gacaacgcca
    27301 ccatcggcaa tatcatgaat ttaagagccc caccattcca gccaccgccc ggcagccaat
    27361 gcaaaatgat cgcaaatatc atgactaata atggcgcaac cacaaaactg gggataacaa
    27421 ccccggtcat tgccagcccc atcacggtat agtcccattt ggtgttttgt tttaatgcgg
    27481 caataacgcc agcactaaca cccagtatta ccgcaaggaa aaatgctgcg gctcccagtt
    27541 tggcagaaac gggaaaactg gatgcaacca ggtcattgac cgaataatct ttatatttaa
    27601 acgatggacc gaaatcaccg tgcgccagtt gtttcaggta gctgaaatac tgtgtcatga
    27661 tcggatcatt aagatgatat ttcgcttcga tattggccat cacttcaggc ggtaaagtac
    27721 gttcgccggt aaaagggctt cccggcgcga ggcgcatcat aaagaacgaa atagtaataa
    27781 gaataaatag cgtcggaatc gcttccagac agcgacgtag aataaatttt aacattgccc
    27841 taccttctgg cctgtgcctt ctgtaatgcg ataaaaatca gacaccgtgg agcaggacac
    27901 tcctgcccca cgtattgcca ttagtgcttc acaatgtaca tattccgggt gtaggtatta
    27961 tccagcggat ctttgccggt atagccacca acccacggtt tcaccagacg cgcattcacg
    28021 tagtaataaa caggaacaat ggccgaatcc ttatccagct gttgttctgc tttagtgtac
    28081 agagctgtgc gctgcgcctc atcagtcact ttcagcgttt ccgccataat gctgtcaaag
    28141 gccgggctct tataatgcgc ggtattcatc gagctgttcg aaagcatggt gttcaggaag
    28201 gaagttggtt cgttgtagtc agcacaccag cctgcacggg ccacatcaaa agtaccctgg
    28261 tgacgggtgt cgaggaacgt tttccactcc tggttaacca gtttgacgtt tacaccaatg
    28321 tttttcttcc acaatgaaga ggcagcaatc gccagctttt tatgcagatc ggaggtgtta
    28381 tacaacaggt tgatggtcaa cggtttgtct gcggtataac ccgcttcagc cagcagtttt
    28441 ttcgcttctt cgttacgttt ttcctggctc cagccaaacc attccggctg agtcaatttt
    28501 gcgccatcag tatacggtgg agtgtaacca taggcgggca tgtcgccctg cgctttcact
    28561 ttattaacaa tgatatcgcg gtccatacct agtttcagtg cggtacgcac acgcacatcg
    28621 ttgaatggcg gtttctggtt gttaatttcg taatagtaag tgcacaggta tggatcaacg
    28681 tgaacttcgt ccgggatctc ttttttcagc ttctggaaca attcgatcgg catgttgtta
    28741 taagtcatgt cgatttcacc actacggtaa cggttgacat cggtaacttc agaagcaata
    28801 ggcaaatagg ttacctggtt aataacggtt ttcgcgttgt tccagtaggt cgggctgcgt
    28861 tcaagaacga ttcgttcgtt tacgacccaa tcttttaagg tataggcacc gttggtgacg
    28921 atattaccag gctgggtcca tttttcgccg aatttctcga tagcggcttt tggcaccggt
    28981 gaagttgatg ggtgaacaag taatttatag aagtacggaa cgggttcact taaggtgact
    29041 tctaatgtgt gatcatcaat agctttcacg ccgagatcgg taatcggttt tttcccttca
    29101 agaatttcat caataccggc gatatgccca tattgcagat aactggcata cggagaagca
    29161 gtgttcggat caacagaacg ttgccagcta tacacaaagt cttgtgctgt gactggcgtg
    29221 ccatcagacc atttcgcatc tttacgcaaa tggaaggtcc agactttcgc gtctttatta
    29281 tcccaggatt cagcgacgcc aggtgctgga tgaccgtcaa gatcgctgac cagtaagcct
    29341 tcaaacaggt ctcggctgat attagactcc ggaacacctt caattttgtg cggatctaat
    29401 gactgaactt ctgaaccatt gttacgtacc agtgtttgtt tttccgccag tgtgacgcct
    29461 gcgggtacat cagctgccag cgcgacattc cctgccatta gcgcagccag aacgccagct
    29521 gctactaaac ttctcttggt gatgttggtc attgtttttt ggactccctc attataatta
    29581 ctggtattaa ccagcatgtg taatcccgga ttgggaccct gcacaggctc ggagactttc
    29641 tgctgtcagg ttactttatc gttacgtatt actatcaccg actttattta ttctggtgat
    29701 tcagacgatc accttatgtc gattcgtact gtgtctcccc tgtcgagaca cattattctt
    29761 tcgcgacgct ctttaattgt taattattct cattaccaat aaagaatcgt ctggcggttg
    29821 cccggaaagt accaaatgcg gttcttactc gccaatacat tttgcaaaat tgttaagcaa
    29881 ttctctttta ttgaccgtat cagaatctat agcttgcatc catgccttaa caaacatctt
    29941 tcaattcaat caaatagaga ttggctaagc atgtctatct tttctgatta cagttccagt
    30001 agtgaaatgc acaataattt aacaattgat tattatttag cactttcctc taccaaaggc
    30061 agtggaatta ctaatatcat ttcaattatt cttcagcaag cccaggatta tgatgtggcg
    30121 aaaataacat gatggtgagg agaagcagtt aaggaaatcc ttataactaa ctgtaatttg
    30181 agaagaatat ttttacctat tccttttgct tttgataact gcgcgggaac aaagtaaaaa
    30241 taatgacgaa gtaaacggag catggcagct ccgtttcatt tgaaaggaat taattaagca
    30301 ggccggggaa aatcccctta ataccagtaa cgataaattc aatccccaat gccatcagca
    30361 atagccccat aatacgcgta atcacgttga tgccggtctg gcgtaaaacc cgtaccagcc
    30421 acggtgccat gcggaacaat ccccagcaac ataaagcgaa caatgcaata gccacaaaga
    30481 aaccaaacag atagctaatg ctgtgataac gcgtacccca gacgatggta gaactgatcg
    30541 cccctggccc cgccatcaac ggcaacgcca atggcaccac accaatgctt tcacgtaccg
    30601 cggtttctga tttttcttgc ttgttctgtt tatcctcgcc aagcttgccg ctgatcatcg
    30661 acatcgctat cgtcaccacc aggatgcccc cggcgatacg gaacgaatca attgatatac
    30721 caaaaagttg cagaatcgtg tcgccgagaa aaagcgagat ccacaagata atggccacag
    30781 acaggttggc tgtaaggtta gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc
    30841 tgataaagac gggaataatc cctaccgggt tgaccagcgc aaataacccg atgaaaaatt
    30901 tgaagtaaac gggaaaatca aaaaaggtct gaatcacggt tagctccgaa gcaaaagccg
    30961 gataatgtta gccataaata aggttgaaaa gacgcgctga caatacgcct tttgacagca
    31021 tttttcacct cctaactact taaaattgct atcattcgtt attgttatct agttgtgcaa
    31081 aacatgctaa tgtagccacc aaatcatact acaatttatt aactgttagc tataatggcg
    31141 aaaagcgatg ctgaaaggtg tcagctttgc aaaaatttga tttggatcac gtaatcagta
    31201 cccagaagtg agtaatcttg cttacgccac ctggaagtga cgcattagag ataataactc
    31261 taatgtttaa actcttttag taaatcacag tgagtgtgag cgcgagtaag cttttgattt
    31321 tcataggtta agcaaatcat caccgcactg actatactct cgtattcgag cagatgattt
    31381 actaaaaaag tttaacatta tcaggagagc attatggctg ttactaatgt cgctgaactt
    31441 aacgcactcg tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt cactcaagag
    31501 caagtagaca aaatcttccg cgccgccgct ctggctgctg cagatgctcg aatcccactc
    31561 gcgaaaatgg ccgttgccga atccggcatg ggtatcgtcg aagataaagt gatcaaaaac
    31621 cactttgctt ctgaatatat ctacaacgcc tataaagatg aaaaaacctg tggtgttctg
    31681 tctgaagacg acacttttgg taccatcact atcgctgaac caatcggtat tatttgcggt
    31741 atcgttccga ccactaaccc gacttcaact gctatcttca aatcgctgat cagtctgaag
    31801 acccgtaacg ccattatctt ctccccgcac ccgcgtgcaa aagatgccac caacaaagcg
    31861 gctgatatcg ttctgcaggc tgctatcgct gccggtgctc cgaaagatct gatcggctgg
    31921 atcgatcaac cttctgttga actgtctaac gcactgatgc accacccaga catcaacctg
    31981 atcctcgcga ctggtggtcc gggcatggtt aaagccgcat acagctccgg taaaccagct
    32041 atcggtgtag gcgcgggcaa cactccagtt gttatcgatg aaactgctga tatcaaacgt
    32101 gcagttgcat ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa
    32161 cagtctgttg ttgttgttga ctctgtttat gacgctgtac gtgaacgttt tgcaacccac
    32221 ggcggctatc tgttgcaggg taaagagctg aaagctgttc aggatgttat cctgaaaaac
    32281 ggtgcgctga acgcggctat cgttggtcag ccagcctata aaattgctga actggcaggc
    32341 ttctctgtac cagaaaacac caagattctg atcggtgaag tgaccgttgt tgatgaaagc
    32401 gaaccgttcg cacatgaaaa actgtccccg actctggcaa tgtaccgcgc taaagatttc
    32461 gaagacgcgg tagaaaaagc agagaaactg gttgctatgg gcggtatcgg tcatacctct
    32521 tgcctgtaca ctgaccagga taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg
    32581 aaaacggctc gtatcctgat taacacccca gcgtctcagg gtggtatcgg tgacctgtat
    32641 aacttcaaac tcgcaccttc cctgactctg ggttgtggtt cttggggtgg taactccatc
    32701 tctgaaaacg ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa
    32761 aacatgttgt ggcacaaact tccgaaatct atctacttcc gccgtggctc cctgccaatc
    32821 gcgctggatg aagtgattac tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc
    32881 ctgttcaaca atggttatgc tgatcagatc acttccgtac tgaaagcagc aggcgttgaa
    32941 actgaagtct tcttcgaagt agaagcggac ccgaccctga gcatcgttcg taaaggtgca
    33001 gaactggcaa actccttcaa accagacgtg attatcgcgc tgggtggtgg ttccccgatg
    33061 gacgccgcga agatcatgtg ggttatgtac gaacatccgg aaactcactt cgaagagctg
    33121 gcgctgcgct ttatggatat ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa
    33181 gcgaaaatga tcgctgtcac caccacttct ggtacaggtt ctgaagtcac tccgtttgcg
    33241 gttgtaactg acgacgctac tggtcagaaa tatccgctgg cagactatgc gctgactccg
    33301 gatatggcga ttgtcgacgc caacctggtt atggacatgc cgaagtccct gtgtgctttc
    33361 ggtggtctgg acgcagtaac tcacgccatg gaagcttatg tttctgtact ggcatctgag
    33421 ttctctgatg gtcaggctct gcaggcactg aaactgctga aagaatatct gccagcgtcc
    33481 taccacgaag ggtctaaaaa tccggtagcg cgtgaacgtg ttcacagtgc agcgactatc
    33541 gcgggtatcg cgtttgcgaa cgccttcctg ggtgtatgtc actcaatggc gcacaaactg
    33601 ggttcccagt tccatattcc gcacggtctg gcaaacgccc tgctgatttg taacgttatt
    33661 cgctacaatg cgaacgacaa cccgaccaag cagactgcat tcagccagta tgaccgtccg
    33721 caggctcgcc gtcgttatgc tgaaattgcc gaccacctgg gtctgagcgc accgggcgac
    33781 cgtactgctg ctaagatcga gaaactgctg gcatggctgg aaacgctgaa agctgaactg
    33841 ggtattccga aatctatccg tgaagctggc gttcaggaag cagacttcct ggcgaacgtg
    33901 gataaactgt ctgaagatgc gttcgatgac cagtgcaccg gcgctaaccc gcgttacccg
    33961 ctgatctccg agctgaaaca gatcctgctg gatacctact acggtcgtga ttatgtagaa
    34021 ggtgaaactg cagcgaaaaa agaagccgct ccggctaaag ctgagaaaaa agcgaaaaaa
    34081 tccgcttaat cagtagcgct gtctggcagt ataaacggcc ccttctgggg ccgttttttt
    34141 gtttacccaa agcaactttt ccataaaccg acagcattag ccttcatcat atttgcgacg
    34201 atgtataacg cctaaacaca gggatattgt actttacagg tcacaagtca acgtcggtgc
    34261 ttaagagccc tgtgaggcgt atagcggcgt taaaaaactg ccgagaaggg tatatagccc
    34321 ggaagaagtg cgtaaaacga actgacagga taaaagtgcc ctgctcaccc tgtcagtaaa
    34381 gaaattctta ttaatcgtgg cgatgccttt cctgaatagc cgttaatgag ccgacttgta
    34441 acgcctcttt atagtgttta cggcatacag aaacgtatcg ttcattacca ccaattacca
    34501 cctgctcacc ttcgttataa ggtctgcctg cttgatcaag acgcagcacc atgcttgctt
    34561 tacggccaca aaaacagatg gtttttaatt caaccagttt gtcggaccat gccagtaagt
    34621 attggctgcc aataaataat tcacctcgaa aatcggtacg taaaccataa caaagtacgg
    34681 gtatatcgag ttgatcgaca acctccgata attcatatac ttgttgtctg gttaaaaact
    34741 ggcattcatc aaccagtacg caatgaattg cctgctgttc atgttccgca cgaatctcat
    34801 caaataatga tgaattttgg ttaaataatt ttgcaggcga tgacaaacct atacgcgaac
    34861 tgactttccc ggcaccaaag cgatcatcaa tttctgccgt atatacgaca gtgcgcatgc
    34921 cgcgttcctg gtaattgtat gaagattgca acaatgctgt agacttaccc gcattcattg
    34981 cggaatagta gaaatatagc tgtgccatca gccacaggcc ctcaatgata tgaaataaag
    35041 ttggctggag tttatcataa ttcgtcagtt tttcagctca acagtatgca cagaagtggg
    35101 tatatgcgtt ctcccttacg aagccttgca taatccttct gagctatcat tacaactgcc
    35161 tcgcttgtta taagcgggta aatgactgct ggtaactatt cacaatcttt aacctgttgc
    35221 gcaagtaata gccctctgtt gacctccagg agatagtgca atactaagtc catgctctta
    35281 ttgcgactgt tctacttttc atcattcgct taatagggaa ttctcgtaaa cacaactaat
    35341 acagaagact gaaaggtcgt cagcctacga taatctcccc ataaaatgtg acatgaatca
    35401 ggaagtttta acctcacgtg ctgcgaaatc atcggtgtaa atagggctat atgccgcgtc
    35461 ttttctggct aattttatga aaagatattt attggcggca caaaataaag aacaattttg
    35521 aattccttac attcctggct attgcacaac tgaatttaag gctctattat tacctcaaca
    35581 aaccacccca atataagttt gagattacta caatgagcga agcacttaaa attctgaaca
    35641 acatccgtac tcttcgtgcg caggcaagag aatgtacact tgaaacgctg gaagaaatgc
    35701 tggaaaaatt agaagttgtt gttaacgaac gtcgcgaaga agaaagcgcg gctgctgctg
    35761 aagttgaaga gcgcactcgt aaactgcagc aatatcgcga aatgctgatc gctgacggta
    35821 ttgacccgaa cgaactgctg aatagccttg ctgccgttaa atctggcacc aaagctaaac
    35881 gtgctcagcg tccggcaaaa tatagctacg ttgacgaaaa cggcgaaact aaaacctgga
    35941 ctggccaagg ccgtactcca gctgtaatca aaaaagcaat ggatgagcaa ggtaaatccc
    36001 tcgacgattt cctgatcaag caataatctt ttgtagattg cacttgctta aaatcccgcc
    36061 agcggcggga ttttttattg tccggtttaa gacaatttaa taagaataag ctataaaaaa
    36121 acggcgtcga ttgctcaacg ccgtttcgtg gataacaccg atacggatgt tacttcttaa
    36181 tgcccatctc ttcttcaagc caggctttaa attccgtgcc aagggtgtta tgacgaatac
    36241 cgtattcaac gaaggcctgc atgtaaccta atttattacc gcagtcatgg ctcttccctt
    36301 tcatatgata ggcttccacc gtttcttttt cgatcagcat atcaattgcg tcggtgagct
    36361 gaatttcatc accagctccc ggaggggttt ttgccagcaa cggccaaata tccgcgctaa
    36421 gtacgtaacg acccacaata gcgagattag acggcgcaac atccgctttc ggtttttcta
    36481 ccacaccaac catcggtacg ctttcacccg gcgctaattc aacgcctttg caatccacaa
    36541 cgccatatgc ggtcacatca gcaaccggtt caaccatgat ctggctatga cccgtttcat
    36601 caaagcggcg gatcatctct gccaggttat cctgtgacaa atcggattca tattcatcca
    36661 gaataacatc aggcaaaata acagctaccg gttcatcacc cactaccggg tgagcacaca
    36721 ataccgcgtg tcccaggcct ttcgccagac cctgacgaac ttgcataata gtcacgtgcg
    36781 gtggacaaat agactgcact tcatcaagca gttgacgttt tacacgtttt tccagcattg
    36841 cttccagttc aaaactggta tcaaagtggt tttcaataga gtttttagat gagtgtgtaa
    36901 ccagcacaat ttcagtaatg ccagccgcaa tacattcatt cacgacgtat tgaattaatg
    36961 gcttatcgac aagtggcagc atctctttcg ggatggcttt cgtcgccggc aacatcctgg
    37021 ttcctaatcc cgcaacgggg ataacggctt ttttgacttt cgtattaatg gcagccattt
    37081 aaattctcct ggactgttcg tgttttgaac gtgttcatat ttctgtatcg catcccagta
    37141 tatcagcacc ccgaaggggc taagttctga aaaggaggcg agttagcata attaagacaa
    37201 ttacttatac gaatggcgac gatagtacca ccagccgaca ttagcaggta atgcaaattt
    37261 agcccgcgtt atcgtttgct cattctgcag acaacatcaa gcgcagtcga ccaccggttc
    37321 cccatatttg gcattgccag gcatcgcatc gctggctcaa ttgattcaaa taagcgttac
    37381 ctaaagtgcc taacggaaca ccattactga tttgcacctg gtgttcgccg gtatttaacg
    37441 tcgcattcag acccgcagaa accagaatga gatttttcag ttcgcgatga taatagccaa
    37501 ctaatagcgg aaactgcccc ggcagattgg cctgacgaag taaatggttt acctgcttca
    37561 ataacgcgcc caactctggc aaccgttgat tttggtgtgc aagctgttcc tgtaataatc
    37621 cgttaaacaa tgcgcgtaat aacaaggcag caagtacgcc attatgtcca gctcgggtga
    37681 catcaaggca ataaaatgcc agatcgtttt ccgaaagtgc ggcaatatca agcaccaggc
    37741 cgggtttgtc cgcggcaacc aattgacgat aattaacccg gcaatgggaa atcacctgct
    37801 gaaccggcgg ttgtagttcc tgtaataatt tcgccgctgc ggcagggtta tcaaccattg
    37861 catcccagtc gcgaaaaagc ctttcctctt cctcaacgcg cgaattaaac atgctgggat
    37921 agagacaggc aaaaaccatc tcgcgcaagc gattcagatc tttaactggt ttcagcaaaa
    37981 catcttcaac gcccagacgt aacgctttgg caatatctgc catattttca gtggcagata
    38041 tcaccagaac cggggtctgg tcgcctctgt tacgtatatg ctccagcagt ttaagcccgt
    38101 tcattcgtgg catcgcgata tcacatatca tcaggtctgg agtgaaacct cccagcaact
    38161 caagggcatc caccccatca gccgccagta ccgttgtcgc tcccaatgag gaaaaccacg
    38221 aatccagaag cgagcgaaat accggctcat cttcaacaat gagaatctgt tttccgacca
    38281 atggctgcgt catgttctct cccctgactg gctttactca atagtggcat gctattgcca
    38341 cctgcgcctg tcagaattaa cttaagtgta gataaaaatt ctggtcaaat gttggtgcgt
    38401 accaacggca aaagttcgtc cattttcttt tccactgcca gctgtccggc cgcaatggca
    38461 gcgtgcgcgc gatggaaatc aagcgtagat atttgcgggc aaacaggttg aatcagaata
    38521 tcgggcggat cacctgccat gcggttcctt ttaaggcggt tctccagcac ctggatagaa
    38581 gtggtcataa tctctgttgc cgttggtgct gtcaccgccc gacgtgtcgt tatgctgcct
    38641 aacctttctt tcagacgcgc atgccacggc agagaatcac cattctcgct attttcttca
    38701 ctgacattaa aggagagcaa atcttgttgc attaaatgag catcgtgctg caggtcgacc
    38761 gctatcacaa tatcagcacc caatgcacgc gtgagcgaaa taggaattgg gttaacgact
    38821 gccccatcaa ccagccagta gccgttatgc gcgactggtg ccatgagtcc tggaatactg
    38881 catgacgcgc gaatagcaag atggagatcg ccttcagtaa accataattc acgcccagta
    38941 cttaaattgg tggcaacagc cgcgaagcga cgggaacaat tttcgatctc tgtttccggc
    39001 attatctcgc ggtattgatt gaagacacgc tcgccgcgca gtaacccacc gcgctgccag
    39061 gagagatcca tcaggcgtaa aacatcccaa taactgaaag aggttaccca atcttccagc
    39121 gcagataatc gatcgcatgc ataggccgcg cccaccagcg aaccaattga acatcctgca
    39181 acgatatcaa tttcaatacc cacttttttt agcgcattaa taacgccaat atgcgaccaa
    39241 cctctcgccg cgccagatcc cagcgccagc cctattttta tctttctcat tattcccgca
    39301 agattcccct ggaatgcaat cgtagccaca tcgctactgc tcggttaaca tagtgctact
    39361 caggcggtta atacgccgtt attgtttcca gggagattat ttgtgtctca gctttgtccc
    39421 tgtggtagtg ctgtcgagta tagcctatgt tgccaccctt atgtgtctgg tgaaaaggtt
    39481 gcacctgatc cagaacatct catgcgttcg cgttactgcg cttttgtgat gcaagacgca
    39541 gattatttaa taaagacctg gcatccatct tgtggagcag cggcgttacg tgccgaattg
    39601 attgccggat ttgcgcatac tgaatggttg ggattgaccg tatttgaaca ttgttggcag
    39661 gacgccgaca atatcggatt tgtcagtttt gttgctcgat ttaccgaagg tggcaaaaca
    39721 ggtgcaataa ttgaacgttc gcgatttttg aaagaaaacg gtcaatggta ctatattgac
    39781 ggtacacgtc cgcagtttgg ccgcaacgat ccctgccctt gtggttcagg taaaaaattt
    39841 aaaaagtgct gcggccaata atggttgacg gtacggttta gcaaacactc tcaacaaggt
    39901 ttttccagca atgcattcac tccaacgtaa agttctgcgt actatttgtc cggaccaaaa
    39961 aggtctgatc gcacgtatta ccaatatttg ctacaagcac gagttaaata tcgtgcagaa
    40021 caatgaattt gttgatcacc gtaccgggcg cttttttatg cgcacggaac tggaagggat
    40081 ttttaatgat tccacccttc tggcggatct cgatagcgca ttgccagaag gctccgtgcg
    40141 tgagctgaat cctgccggtc gtcgccggat agtgattctg gtcactaaag aagcgcattg
    40201 ccttggcgat ttgttgatga aagccaatta cggcggcctg gatgtcgaaa tcgcggcagt
    40261 tattggtaac cacgatactt tacgttctct ggttgagcgt tttgatattc cgtttgagct
    40321 ggtaagccat gaagggttaa gccgcaacga gcacgatcaa aagatggcgg acgccattga
    40381 tgcttatcaa cctgactatg tggtgctggc gaagtatatg cgggtattaa cgccggaatt
    40441 tgtggcacgc ttcccgaata aaatcatcaa tattcaccat tcattcctgc ctgcgtttat
    40501 cggcgcacgt ccttatcacc aggcctatga acgtggtgtg aagattattg gcgcaaccgc
    40561 tcactatgtg aatgacaatc tggacgaagg cccaatcatc atgcaggacg ttattcatgt
    40621 cgatcatacc tacacagctg aagatatgat gcgcgcaggt cgtgacgtcg agaaaaacgt
    40681 cttaagtcgt gcgctataca aagtactggc acagcgcgtc tttgtttacg gtaatcggac
    40741 gattattctt taatcgccag caaaaataac tggttacctt taatccgtta cggatgaaaa
    40801 ttacgcaacc agttcatttt tctcaacgta acactttaca gcggcgcgtc atttgatatg
    40861 atgcgccccg cttcccgata agggagcagg ccagtaaaaa gcattacccc gtggtggggt
    40921 tcccgagcgg ccaaagggag cagactgtaa atctgccgtc atcgacttcg aaggttcgaa
    40981 tccttccccc accaccatca cttttcaaaa gtccgtaaac tctcaaacga atccgcaatt
    41041 aaatattctg cccaagcggg gaaggatgag aagcttcgac caaggttcga cttgagcgcc
    41101 agcgagagag cgttgccgca ggcaacgacc cgaagggcga agcgcgcagc gctgagtaat
    41161 ccttccccca ccaccatcgc ttccttaaat aatcaaaacc tgttactccg agctaatttt
    41221 gcccgatggc accacgttta ccgggcctac aatatcctcc cctgtaggtc ggataagcga
    41281 agcgcatccg acaactcacc ccgcaccgtg cccataaaaa aaccccgccg aagcagggtc
    41341 tatgtgaact aaaattcgct tagtgacgag cgcgcaccag ctggtactta cgtgtcagat
    41401 actccaccgg tacgctccag atgtgtacca gacgcgagaa cgggaacagc aggaataacg
    41461 tcatccccag caccaggtgc agacggaaga taaacgccac accatcgagg tgttgagaag
    41521 cgccaccgtg gaaggtcacc accgactgcg cccagccaac cagtttcatc atctcgctac
    41581 cgtccatatg ctgagcggag aacggaatgg tcaacaggcc cagcgcgcac tggataacga
    41641 gcagcgacag gatcaggata tccgctccgg tagtggttgc acgcacgcgt gggctgaaca
    41701 gacgacgctt cagcaacagc acgccgccaa tcagacacag cacgccgctg gcaccaccag
    41761 caaacatcgc cattttctgt ttcacttcaa tcggcagcca ggcttcatac atccagtgcg
    41821 gcgtcagcat accgaagaag tgaccaacaa aaatccccag aataccgata tggaacaggt
    41881 tcgacgccag gttcatccct ttgcgatcca gcatctggct ggacgccgcg cgccaggtgt
    41941 actgcccgta gtcataacgc agccagctac caatcaggaa gaccgccccg gctatgtacg
    42001 ggtagatatc aaagaagaac atattcagga attgcattag tgctgtcctc cggtggtgat
    42061 attcagatat tgcggcgcga cggcaccggc aaagcgacgc tgatgagcag tgattgctga
    42121 atcaccgcag cctttgtcag caaagaattt aacctgctct tcttcccaga cagcatccag
    42181 cgcctgcggc gtatcatcgc gcgcttcgtc ggcaattttt tccgccactt tgtcgctgtc
    42241 gatagcggta ttggccaatt tcagcagcag atcaaacatc acggcataac ggctttcacg
    42301 ctgttgcaga cgcgcgctca gcaatgccag aatcggcgcg atatctttca aaccttccac
    42361 ggcttcgctt tgcggcagct gcgacaggta ctccagatac agcggcagat ggtccggtaa
    42421 ttcgcggctg tttaactgca agccgtgctg ctcgtactgc gccagcaggt ccaccatcgc
    42481 ctggccgcgg tcacgggatt caccgtgaac atgttcaaat agcaacagtg acgtggcgcg
    42541 gccacggtcg aacagttcgc tgtactgggc ctgggcatcg agcggatcca tcgccgttaa
    42601 atcgcgcagg aaaatgccca gcgcatgggc atcttctttc gacagatttt tcgacgcagc
    42661 aatcgcctca aacatctctt gttgatgctg ccataaggca gcatccggat attcaaggag
    42721 acgcgataca atcacgagtt cgatcatgga tgcggctccg ttttgctggt cacatcgatg
    42781 gcatcgatac gacggctgtt gaacagattg aatttggtat ctgaaccgtg gcagccatca
    42841 ccaaaggtaa agccgcagcc atttttctcc gggaaggctt cccgtgccag ttcacgatga
    42901 ctactcggca ccacaaagcg atcttcgtag ttagcaatcg ccagataacg gtacatctcc
    42961 tgtgcctggg cttcggtcag accgacctct tccagcgcac gggtatcaac tttaccgtca
    43021 acggtttcag cacgtttgta atgacgcatc gccagcatac gtttcagtgc gcgcagtacc
    43081 ggtttggtat caccggcggt cagcagattc gccagatact gtaccggaat acgcaagctt
    43141 tcgacgtctg gcagaatgcc gttgctaccc agctcacccg cgtctgctgc agactgaatc
    43201 ggagacagag gcggcacgta ccagaccatc ggcagtgtgc gatattctgg atgcagcggc
    43261 agcgccagtt tccattccat tgccatttta taaaccggcg actgctgtgc ggcttcaata
    43321 acgctcagcg gaataccgtc tttaatcgcc tgctcgatga ctttcggatc gttcggatcg
    43381 aggaacacct ccagctgacg ctggtaaaga tctttctcgt tctcggtgct ggctgcacgt
    43441 tcaatcgcgt cggcatcgta cagcagcacg ccaagataac ggatacgacc gacacaggtt
    43501 tctgagcaca cggtcggctg acccgcttca atacgcggat agcagaagat gcacttctca
    43561 gacttaccgc tcttccagtt gaagtagatt tttttgtacg ggcatccggt gatgcacata
    43621 cgccagccac ggcatttatc ctggtcgatc aggacgatgc catcttcttc acgcttgtaa
    43681 atcgcaccgc tcgggcaggt cgccacacat gccgggttca ggcagtgttc gcacaggcgc
    43741 ggcaaataca tcataaaggt gttttcgaac tggctataca tcgccttctg gatgttgtcg
    43801 aagttcttgt ctttcgccag tttgtcaaac tcaccaccca ggtcatcttc ccagttcggc
    43861 cctttttcga ttttcgccat ccgttccccg gtaatcagcg aacgcggacg ggcaatcggc
    43921 tgcgatttgc tgccttccgg cgcggtatgc aggttctgat agtcaaaatc gaacggctcg
    43981 taataatcgt cgatccccgg cagatgcggg ttagcgaaga ttttacccag caacatggca
    44041 cggttaccca tgcgcggctg cagtttgccg ttgattttac ggatccagcc gcctttgtat
    44101 ttttcctggt tttcccagtc agtcgggaag ccctgacccg gcttggtttc cacgttgttg
    44161 aaccacgcgt attccacgcc ttcacggctg gtccagacgt ttttacaggt aactgaacag
    44221 gtgtggcagc cgatgcactt atcgagattc agcaccatgc cgacttgtga acgaattttc
    44281 attttacgct ctcctgtacc tggtcattgc cttcgccatc taaccagtca atgttcttca
    44341 tcttacgcac tacaacaaac tcatcgcggt tagaacctac ggtgccgtag tagttaaagc
    44401 cgtatgccag atgggcatag ccgccgatca tatgcgtcgg tttcggcgtg atacgggtga
    44461 ccgagttatg gataccacca cgctgttggg taatttccga accaggcagg ttaacgatac
    44521 gttcctgcgc gtggtacatc atggtcatcc ctgccggaac acgctggctg acaaccgcac
    44581 gggcagtcag agcaccgttg ctgttgaaga cttcaatcca gtcgttatcg gcgataccca
    44641 gatctttggc atcggcttca ctcaaccaga ccaccggacc accgcgacct aaagtcagca
    44701 tcagcaggtt gtcgctgtag gtggagtgga taccccactt ctggtgcggc gtcaggaagt
    44761 tgagcgcttt ttccgggttg ccgttggatt tctggcccat cacttctttc accgaacggg
    44821 tgtcgatcgg cggacgataa accagcaggc tttcaccgaa atcacgcatc cattggtgat
    44881 cctgatacag ctgctgacgg ccagagagcg tacgccacgg gatcagctcg tgaacgttgg
    44941 tgtaaccggc gttgtaagaa acgtgttcat cttcaaggcc agaccaggtc gggctggaga
    45001 tgattttgcg cggctgtgcc tgaatatcgc ggaagcgaat cttctcgtct tctttattca
    45061 gcgccagatg cgtatggtca cgaccggtaa actcgctcag ggcagcccag gctttcacgg
    45121 ctacctgacc gttggtttcc ggtgccagcg tcaggatcat ctctgccgca tcaattgcgg
    45181 tgttcagcat cggctggcct ttcgccggac cttccgcttt ggtgtagttg agcttacgca
    45241 gcagatccat ctcgctctgg gtgttccagg caatcccttt accgccatta ccgattttct
    45301 ccatcagcgg gccgatagag gtaaagcgtt cgtaagtcgc cggataatcg cgctctacga
    45361 ccataatgtg tggcgcggtt ttaccaggga tcaggtcgca ctcgcctttt ttccagtctt
    45421 tcacatccag cggctgcgcc agttcagcgg cagagtcgtg ctggataggc agcgtgacga
    45481 tgtcggtttc tttacccaga tgaccgacgc acacttcgga gaatttcttc gcgatggctt
    45541 tgtaaatttc ccagtcgctt ttcgcttccc aggccggatc gaccgccgca gacagcgggt
    45601 gaataaacgg atgcatatcc gaagtattca tgtcgtcttt ttcgtaccag gtcgccgtcg
    45661 gcaaaatgat gtcggaatag agacaggtgc tcgacagacg gaagtccagc gtaaccacca
    45721 gatccagctt gccttccaga ccattgtcct gccagtccac ttcttccggc ttcacgccgc
    45781 cctgttgccc cagatcttta ccctggatac cgtgctccgt ccccagcagg tacttgagca
    45841 taaactcatg acctttaccg gaagaaccga gcaggttaga acgccagatg aacaggttgc
    45901 gcgggtggtt tttaccgttt tctggttgtt ctgccgcaaa acggatggaa ccctctttca
    45961 gggatttcac cgtatagtcc accggattca tcccggcttt ttccgcttcg ccagcgatag
    46021 tcagcgggtt agtgcctaac tgcggtgcag acggcagcca gcccatgcgt tccgcacgga
    46081 cgttaaagtc gatcaagtgt ccggtatagc gggatttgtc cgtcatcggt gacagcaact
    46141 cttccgccgt gacggtttca taacgccact ggctggagtg gttatagaaa taagaagtgc
    46201 tgttcatgtg acgcgccgga cgctgccagt caagggcaaa cgccagcggc tgccagccgg
    46261 tttgcggacg cagtttttcc tgacctacat agtgcgccca gccgcccccg ctctgaccga
    46321 cacagccgca gaaaatcagc atgttgatca gaccacgata gttcatatcg aggtgatacc
    46381 agtggttcag ccccgcaccg acgataatca tcgaacgacc gtgcgtttta tcagcgttat
    46441 cggcaaattc acgggcgatg cgaataatct ggctgcgaga aacgccggta atctgctcgg
    46501 cccaggccgg ggtataagct ttcacatcgt catagctggt tgcacagtta acgtcgttca
    46561 ggccacgttc cagaccgtag tttgccagcg tcagatcata aacggtggtc accagggcgg
    46621 tgctgccatc agccagttgc aggcgtttca ccggcagttt gtgcagcagc acgttttcca
    46681 gttccacttt gttgaagtgt tccgtgccgt cgccaccaaa gtacgggaaa cctacctcag
    46741 cgatctcatc ctggctaccc agcaggctca gttgcagctc ggtttcttcg ccagttttgc
    46801 cgtcgcgctg ctcaagattc catttgccct tctcgcccca gcggaagcca atagaaccgt
    46861 tcggcgcaac catttcgcca ttggtattaa aggcgacagt tttccattcc ggattgtttt
    46921 cctggcccag cgcatcaacc agatcagcag cgcgcagcat acgacctgca gcgtagtaac
    46981 cgtcgcgttc ttccagcatc accagcatcg gcatgtcggt gtagcgacgc acatagtcgg
    47041 tgaaatactg gcttgggttg tcgaggtgga attcacgcag cattacgtgg cccatcgcca
    47101 gcgccattgc cgcatcggtg ccctgtttcg gtgccagcca cagatcgcac agtttggcga
    47161 tttcagcgta gtctggtgtg acggcaacag ttttggtccc tttgtaacgc acttcagtaa
    47221 agaagtgagc atccggggta cgcgtctgcg gtacgtttga cccccaggcg atgatgtagc
    47281 tggagttgta ccagtcagca gattccggta cgtcagtttg ctcgccccag gtttgcggag
    47341 acgcaggagg caagtcgcag taccagtcgt agaagcttaa gcaagtaccg ccaatcagcg
    47401 agagatagcg tgcacccgat gcgtaagaaa ccatcgacat tgccggaatt ggcgagaaac
    47461 cagcaacacg gtccgggccg tagtttttga tggtgtaaac gttagatgcg gcgatcagtt
    47521 cgttcacctc ctgccaggaa gaacgaacaa atccaccgcg tccacgcgcc tgcttaaagc
    47581 ttttcgcttt atcggcgtct tcaatgatag aagcccatgc ctcaaccgga tcgctatgca
    47641 gcgccttcgc ttcacgccac attttcatca ggcgtttgcg catcatcggg tatttcaggc
    47701 ggttggcact gtaaagatac caggagtagc tggcaccgcg cgggcagccg cgaggttcat
    47761 ggtttggcag atccggacgg gtacgcggat agtcagtctg ctgggtttcc caggtgacca
    47821 gaccgttttt gacgtagatt ttccagctgc aggagccggt gcagtttacc ccgtgggtag
    47881 agcggacgat tttgtcatgc tgccaacgct ggcgatatcc atcctcccag tcacggttgg
    47941 tattgagaag ctggccatgc ccatcggcaa aggtttcacc cttctgtttg aagtagcgaa
    48001 accggtccag gaatttactc atcggttttc tcctgtggga gcctgtcggc cctctgataa
    48061 atcgacattg ctaatgttta agggcaaggt aacgctctga aacagcgtgg gaattgataa
    48121 cgatcaagtg tgatggggat gaaaaataaa gtaaattcct aagatacc
//
LOCUS       DAZGSP010000029        48042 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZGSP010000029 DAZGSP010000000
VERSION     DAZGSP010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.029"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..1446)
                     /locus_tag="ACHK74_001796"
                     /pseudo
     CDS             complement(1..1446)
                     /locus_tag="ACHK74_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367409.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(1569..2021)
                     /gene="tabA"
                     /locus_tag="ACHK74_001797"
     CDS             complement(1569..2021)
                     /gene="tabA"
                     /locus_tag="ACHK74_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFP0220629.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTVETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(2166..2759)
                     /gene="bdcR"
                     /locus_tag="ACHK74_001798"
     CDS             complement(2166..2759)
                     /gene="bdcR"
                     /locus_tag="ACHK74_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP0220630.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGGAIKTILKE"
     gene            2830..3543
                     /gene="bdcA"
                     /locus_tag="ACHK74_001799"
     CDS             2830..3543
                     /gene="bdcA"
                     /locus_tag="ACHK74_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0220631.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMSRGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3674..4069
                     /gene="yjgH"
                     /locus_tag="ACHK74_001800"
     CDS             3674..4069
                     /gene="yjgH"
                     /locus_tag="ACHK74_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP0220632.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            4350..4484
                     /gene="pyrL"
                     /locus_tag="ACHK74_001801"
     CDS             4350..4484
                     /gene="pyrL"
                     /locus_tag="ACHK74_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFP0220633.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            4488..5423
                     /gene="pyrB"
                     /locus_tag="ACHK74_001802"
     CDS             4488..5423
                     /gene="pyrB"
                     /locus_tag="ACHK74_001802"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFP0220634.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            5436..5897
                     /gene="pyrI"
                     /locus_tag="ACHK74_001803"
     CDS             5436..5897
                     /gene="pyrI"
                     /locus_tag="ACHK74_001803"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFP0220635.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5970..6356
                     /gene="ridA"
                     /locus_tag="ACHK74_001804"
     CDS             5970..6356
                     /gene="ridA"
                     /locus_tag="ACHK74_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFP0220636.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(6562..9258)
                     /gene="mgtA"
                     /locus_tag="ACHK74_001805"
     CDS             complement(6562..9258)
                     /gene="mgtA"
                     /locus_tag="ACHK74_001805"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFP0220637.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAERVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(9399..9452)
                     /gene="mgtL"
                     /locus_tag="ACHK74_001806"
     CDS             complement(9399..9452)
                     /gene="mgtL"
                     /locus_tag="ACHK74_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFP0220638.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            9637..10584
                     /gene="treR"
                     /locus_tag="ACHK74_001807"
     CDS             9637..10584
                     /gene="treR"
                     /locus_tag="ACHK74_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFP0220639.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLNPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            10703..12124
                     /gene="treB"
                     /locus_tag="ACHK74_001808"
     CDS             10703..12124
                     /gene="treB"
                     /locus_tag="ACHK74_001808"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFP0220640.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            12174..13829
                     /gene="treC"
                     /locus_tag="ACHK74_001809"
     CDS             12174..13829
                     /gene="treC"
                     /locus_tag="ACHK74_001809"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFP0220641.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIISRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            14223..16361
                     /gene="nrdD"
                     /locus_tag="ACHK74_001810"
     CDS             14223..16361
                     /gene="nrdD"
                     /locus_tag="ACHK74_001810"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFP0220642.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            16519..16983
                     /gene="nrdG"
                     /locus_tag="ACHK74_001811"
     CDS             16519..16983
                     /gene="nrdG"
                     /locus_tag="ACHK74_001811"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFP0220643.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(17028..17414)
                     /gene="cybC"
                     /locus_tag="ACHK74_001812"
     CDS             complement(17028..17414)
                     /gene="cybC"
                     /locus_tag="ACHK74_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFP0220644.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTMMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(17597..18949)
                     /gene="pmbA"
                     /locus_tag="ACHK74_001813"
     CDS             complement(17597..18949)
                     /gene="pmbA"
                     /locus_tag="ACHK74_001813"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFP0220645.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            19043..19594
                     /gene="yjgA"
                     /locus_tag="ACHK74_001814"
     CDS             19043..19594
                     /gene="yjgA"
                     /locus_tag="ACHK74_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFP0220646.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(19745..21118)
                     /gene="mpl"
                     /locus_tag="ACHK74_001815"
     CDS             complement(19745..21118)
                     /gene="mpl"
                     /locus_tag="ACHK74_001815"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFP0220647.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            21294..22292
                     /gene="fbp"
                     /locus_tag="ACHK74_001816"
     CDS             21294..22292
                     /gene="fbp"
                     /locus_tag="ACHK74_001816"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFP0220648.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(22325..23320)
                     /gene="yjfF"
                     /locus_tag="ACHK74_001817"
     CDS             complement(22325..23320)
                     /gene="yjfF"
                     /locus_tag="ACHK74_001817"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFP0220649.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(23307..24329)
                     /gene="ytfT"
                     /locus_tag="ACHK74_001818"
     CDS             complement(23307..24329)
                     /gene="ytfT"
                     /locus_tag="ACHK74_001818"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFP0220650.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(24343..25845)
                     /gene="ytfR"
                     /locus_tag="ACHK74_001819"
     CDS             complement(24343..25845)
                     /gene="ytfR"
                     /locus_tag="ACHK74_001819"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFP0220651.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGFLRRKEMEKRATELMTSYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(26155..27111)
                     /gene="ytfQ"
                     /locus_tag="ACHK74_001820"
     CDS             complement(26155..27111)
                     /gene="ytfQ"
                     /locus_tag="ACHK74_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFP0220652.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            27421..27951
                     /gene="ppa"
                     /locus_tag="ACHK74_001821"
     CDS             27421..27951
                     /gene="ppa"
                     /locus_tag="ACHK74_001821"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFP0220653.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(28031..28381)
                     /gene="chpB"
                     /locus_tag="ACHK74_001822"
     CDS             complement(28031..28381)
                     /gene="chpB"
                     /locus_tag="ACHK74_001822"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFP0220654.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(28375..28626)
                     /gene="chpS"
                     /locus_tag="ACHK74_001823"
     CDS             complement(28375..28626)
                     /gene="chpS"
                     /locus_tag="ACHK74_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFP0220655.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            complement(28838..29179)
                     /gene="ytfP"
                     /locus_tag="ACHK74_001824"
     CDS             complement(28838..29179)
                     /gene="ytfP"
                     /locus_tag="ACHK74_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFP0220656.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(29182..32961)
                     /gene="tamB"
                     /locus_tag="ACHK74_001825"
     CDS             complement(29182..32961)
                     /gene="tamB"
                     /locus_tag="ACHK74_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFP0220657.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKLKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGILSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPQTTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(32958..34691)
                     /gene="tamA"
                     /locus_tag="ACHK74_001826"
     CDS             complement(32958..34691)
                     /gene="tamA"
                     /locus_tag="ACHK74_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFP0220658.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            34897..35535
                     /gene="msrA"
                     /locus_tag="ACHK74_001827"
     CDS             34897..35535
                     /gene="msrA"
                     /locus_tag="ACHK74_001827"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP0220659.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            35858..37201
                     /gene="paeA"
                     /locus_tag="ACHK74_001828"
     CDS             35858..37201
                     /gene="paeA"
                     /locus_tag="ACHK74_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP0220660.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(37263..37469)
                     /gene="ytfK"
                     /locus_tag="ACHK74_001829"
     CDS             complement(37263..37469)
                     /gene="ytfK"
                     /locus_tag="ACHK74_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFP0220661.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            37794..38351
                     /gene="ytfJ"
                     /locus_tag="ACHK74_001830"
     CDS             37794..38351
                     /gene="ytfJ"
                     /locus_tag="ACHK74_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFP0220662.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(38341..39081)
                     /gene="cysQ"
                     /locus_tag="ACHK74_001831"
     CDS             complement(38341..39081)
                     /gene="cysQ"
                     /locus_tag="ACHK74_001831"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFP0220663.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEARQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            39271..41214
                     /gene="cpdB"
                     /locus_tag="ACHK74_001832"
     CDS             39271..41214
                     /gene="cpdB"
                     /locus_tag="ACHK74_001832"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFP0220664.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALARAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(41332..41712)
                     /gene="ytfH"
                     /locus_tag="ACHK74_001833"
     CDS             complement(41332..41712)
                     /gene="ytfH"
                     /locus_tag="ACHK74_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP0220665.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGGLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            41801..42661
                     /gene="qorB"
                     /locus_tag="ACHK74_001834"
     CDS             41801..42661
                     /gene="qorB"
                     /locus_tag="ACHK74_001834"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP0220666.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKTAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEVGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAE
                     SVSHLFNVNN"
     gene            42769..43734
                     /gene="ytfF"
                     /locus_tag="ACHK74_001835"
     CDS             42769..43734
                     /gene="ytfF"
                     /locus_tag="ACHK74_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP0220667.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            43842..44504
                     /gene="ytfE"
                     /locus_tag="ACHK74_001836"
     CDS             43842..44504
                     /gene="ytfE"
                     /locus_tag="ACHK74_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFP0220668.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(44549..45961)
                     /gene="cycA"
                     /locus_tag="ACHK74_001837"
                     /gene_synonym="dagA"
     CDS             complement(44549..45961)
                     /gene="cycA"
                     /locus_tag="ACHK74_001837"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFP0220669.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(46270..46890)
                     /gene="fklB"
                     /locus_tag="ACHK74_001838"
     CDS             complement(46270..46890)
                     /gene="fklB"
                     /locus_tag="ACHK74_001838"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP0220670.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            47109..47747
                     /gene="ytfB"
                     /locus_tag="ACHK74_001839"
     CDS             47109..47747
                     /gene="ytfB"
                     /locus_tag="ACHK74_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFP0220671.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(47731..47838)
                     /locus_tag="ACHK74_001840"
                     /pseudo
     CDS             complement(47731..47838)
                     /locus_tag="ACHK74_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(47882..>48042)
                     /locus_tag="ACHK74_001841"
     CDS             complement(47882..>48042)
                     /locus_tag="ACHK74_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP0220672.1"
                     /translation="FRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEP
                     TEMIQATA"
BASE COUNT        11788 a        12835 c        11930 g        11489 t
ORIGIN      
        1 tttgtttttt cttcattacc ataaacatat cctaactcat taagatacag ctgttcgccc
       61 tcataatctt taagtaattg atagaaatac cacttagcaa aatcaggttg cttcatgaaa
      121 aaatccttca aaaaaaatat catttactaa aatgctgaca ggcacttcat taaatagcaa
      181 cttaacatca tcaaataacc ttagtattaa gcgtgatttt tcttgattgc ttaagttaaa
      241 atcattgaca tatgcaacca acttatccca aatcaattcc tgtaatttac ggctattttt
      301 atagtgttct ttatcgagat tgaaaaataa ggtctcagct attttaattt tagattgatc
      361 gcagctaaaa tctttagatt gatcgcggct aaaatcaata tgatttatca cggagcatgc
      421 atactttatc ttttcagaat caccaaaata ttgcattctt ttattaaaac accaatccag
      481 tattcttaca tcattaatat atgggaattt taaaaaaatc tccagaatcg aatcgatgac
      541 cggtgtaagg ttaacttttg catcatctaa ggaagcaata agaatatgag acaggttaac
      601 tttgatgtca ttaatttcat tgtcagtagc tttcttatta ttaagaagat aattatttac
      661 tgcgttaagg tcatctattt ctttcagtat ccctttttca ccaagcaagt atgaaatcga
      721 ttcattacat acataaggca ttaatagcag atgttctttc cttattgcta ataatgcatt
      781 attcaatggt ttttctaata aatccgtaat cgtagaatca actgaatctt tacaactcac
      841 ctgcgagtta ttcaagtcct gagaattatt taactcctgc ggaatattta atagacgagc
      901 tatttccttg ttagcttcgc gttggtaaca cgccagaatc ttgttagtaa tcaggtgtaa
      961 tgaactctca tcttttcttt caaaggactt cccttcataa cgattagtcg aaccatcagg
     1021 ttgtggaaat ataatgactg tatgtccttc acgaatccca ttattaaaag ctgaggcaag
     1081 tgtaatttct atttccacct tagataaatc cgctcgctcc ccttcaggat tattcataac
     1141 aaacttcatt atctcactac catcattatt tttgatataa gatgaggact taatatgagc
     1201 catcatattt ctacccgctt cgttggagcg tttgctaaaa tccacataac ccagggtaaa
     1261 tatcttagcc aaaaactcca aaaatgctct gggagaaaga atattgcttc tgctattctg
     1321 ccctccggca attcgggcaa aatcattcat tcccacatac caggtatcta agtcttcatt
     1381 tttttgttta caattgtcgg ctgatgtaat gttttgagaa aaattccaac ctgatatttt
     1441 gctcatgacg gactccttga gttaataaga acgtctaagc attacaaatt ctactcacaa
     1501 aaaaatatca aaaaacgctc ttttcacaaa ctcgccgtaa acctggagtt atttacggcg
     1561 aaaagtcatc acgccatcag catcttcact actgctttgc gaacctgggc tggcgcgccc
     1621 actgcgcaca gcggtttatg cacttccccc ggataaaaca caacaaaatc accttcatta
     1681 aggataactg ttttctcatc aacgccttcc ggcaaaaatg cgatgtcttt atcagctaac
     1741 cagtcggtat ccggcgtgcc tgcaggttgc gtgctgaagg tcatgccttc ctgacctttt
     1801 aacacaatct gaatgtcgag atagcgggca tgatactccg cacggcgagc ttcgtacggc
     1861 tcggtcatat cttccgagat aagataaaac agacgattgc cttcgatatc gtgcttgccc
     1921 tttggcgttt ctaccgtaac gtgtgctttg atatgctcaa tcgcctggcg taactcctgg
     1981 ggtagccacg gctgaagatt atgaatattt ccgatgatca tcttttccac tccttaatta
     2041 aaacaacgtt tcatttctat cgttatacga aaagatgatc aattccgcta gccttaacgc
     2101 gtgaattatt gtcttatcac aacttatcgg caggcgcgtt cccgcctttc gggtcaaggc
     2161 ccgcatcact ccttgagaat agtttttatc gctccccccg ccagtgcagc tgttgcacag
     2221 agttgttcga gggagtgccc ctctcgtgcc ttcgcagaaa gccctgacat cacggtactc
     2281 ataaaatcag tcacacattg tgcgctttgt ggatgccgcc tggcgatata gtcataaatg
     2341 gccgtttctg cggcgtgata atattgaacg gcaatatcac gcgcttgtgg atcatgacta
     2401 tgaatgcctt caagaaccat acagccagcg cagccgccgt tttggctata tctgcgcgcc
     2461 gcttctttta atacctcagc caggcactcc cctaccggac gatcatcacg aagaatatcg
     2521 acaagcggaa tagcttccgt accgacatat tcattgagta cacggctaaa taacccagcc
     2581 ttactgccaa aagccgcgta gaggctcggg gggttaatac caagataatc agtaacttca
     2641 gcaacactga cagcatcgaa acctttttgg tgaaaaagca cttttgccgc agagactgcc
     2701 tgctcaggag cgaaacgtct gggacgacct ggaacgcgag attgtttttt agtgaccatt
     2761 acaaaacttg ttgacagaaa gttaaaacag ttttgtaatg catgttacat aataaatcaa
     2821 ggagtcctta tgggcgcttt tacaggtaag acagttctca tcctcggtgg tagccgtgga
     2881 atcggtgccg ctatcgtacg tcgtttcgtc accgatgggg ccaatgtacg attcacctat
     2941 gcggggtcga aagatgccgc tgaacgcctg gcacaagaga ctggagcaac agcagtattc
     3001 acagatagtg ctgacagaga cgctgttatt gatgtcgttc gtaagagcgg cgcattggat
     3061 atcctggtgg taaatgcagg tattggcgtc tttggcgatg ccctggaatt aaatgccgac
     3121 gatattgatc gccttttcaa aatcaatatt catgctcctt accatgcctc tgttgaagcc
     3181 gcccggcaga tgcccgaagg cgggcgcatc ttaatcatcg gctccgtgaa tggcgatcgt
     3241 atgcctgttg caggcatggc tgcttatgcc gccagtaaat ctgccctaca aggcatgtcg
     3301 cgcgggctgg cccgtgattt tggaccgcgt gggatcacca tcaacgtcgt ccagccaggg
     3361 ccaattgata ccgacgctaa tcccgccaac gggccaatgc gcgatatgtt gcatagtttg
     3421 atggctatca aaagacatgg gcaaccggaa gaggtcgctg gtatggtcgc atggttagca
     3481 gggccagaag ccagttttgt taccggcgcg atgcatacca ttgatggcgc gtttggcgca
     3541 taaccgacta cgctcaatta agcccagcca ttatccatga tgtctgggtt ttgtttactc
     3601 acgtcgtccg ctaaaagcgg ctcctggtaa atataaatct tctgttcatg tcgtaaatct
     3661 tgaggataac caaatggtag aaagaaccgc tgttttccct gcaggccgac attcactgta
     3721 tgctgaacat cgttattctg cggctattcg ttccggcgat ttgctgtttg tttccgggca
     3781 agtaggaagt cgagaggacg gaacaccaga acccgatttt cagcaacaag tcagactggc
     3841 atttgataat ttgcatgcga ccctggctgc tgcgggatgc acttttgacg atatcattga
     3901 tgttacgagc ttccataccg atccagaaaa ccaatttgaa gacatcatga cggtgaaaaa
     3961 tgaaatattt agcgccccac cttatccaaa ctggacggcg gtgggtgtta catggctggc
     4021 aggctttgat tttgaaatta aagtgatagc gcgcatccct gagcagtaag caatagtgtt
     4081 agccgttcgt tttcacactc cgccctataa gtcggatgaa tggaataaaa tgcatatctg
     4141 attgcgtgaa agtgaaaaag aaaaaagcag gaaatgtctg caattattga taccgaacga
     4201 ccgttcccct gcagaatcac atcaaataaa aatgcatata ccttgacttt taattcaaat
     4261 aaaccgtttg cgctgacaaa atattgcatc aaatgcttgc accgcttctg acgatgagta
     4321 taatgccgga caatttgccg ggaggatgta tggttcagtg tgttcgacat tttgtcttac
     4381 cgcgtctgaa aaaagacgct ggcctgccgt ttttcttccc gttgatcacc cattcccagc
     4441 ccctcaatcg aggggctttt ttttgcccag gcgtcaggag ataaaagatg gctaatccgc
     4501 tatatcagaa acatatcatt tccataaacg accttagtcg cgatgacctt aatctggtgc
     4561 tggcgacagc ggcgaaactg aaagcaaacc cgcaaccaga gctgttgaag cacaaagtca
     4621 ttgccagctg tttcttcgaa gcctctaccc gtacccgcct ctctttcgaa acatctatgc
     4681 accgcctggg tgccagcgtg gtgggcttct ccgacagcgc caatacatca ctgggtaaaa
     4741 agggcgaaac gctggccgat accatttcgg ttatcagcac ttacgtcgat gcgatagtga
     4801 tgcgtcatcc gcaggaaggt gcggcgcgcc tggccaccga gttttccggc aatgtaccgg
     4861 tactgaatgc cggtgatggc tccaaccaac atccgacgca aaccttgctg gacttattca
     4921 ctattcagga aacccagggg cgtctggaca atctccacgt cgcaatggtt ggtgacctga
     4981 aatatggccg caccgttcac tccctgactc aggcgttagc gaagttcgac ggcaaccgtt
     5041 tttacttcat cgcgccggac gcgctggcaa tgccgcaata cattctggat atgctcgatg
     5101 aaaaagggat cgcatggagt ctgcacagct ctattgaaga agtgatggcg gaagtagaca
     5161 tcctgtacat gacccgcgtg caaaaagagc gtctggaccc gtccgagtac gccaacgtga
     5221 aagcgcagtt tgttcttcgc gccagcgatc tccacaacgc caaagccaat atgaaagtgc
     5281 tgcatccgct gccgcgtgtt gatgagattg cgacggatgt tgataaaacg ccacacgcct
     5341 ggtacttcca gcaggcaggc aacgggattt tcgctcgcca ggcgttactg gcactggttc
     5401 tgaatcgcga tctggtactg taaggggaaa tagagatgac acacgataat aaattgcagg
     5461 ttgaagctat taaacgcggc acggtaattg accatatccc cgcccagatc ggttttaagc
     5521 tgttgagtct gttcaagctg accgaaacgg atcagcgcat caccatcggt ctgaacttgc
     5581 cttctggcga gatgggccgc aaagatctga tcaaaatcga aaataccttt ttgagtgaag
     5641 atcaggtaga tcaactggca ttgtatgcgc cgcaagccac ggttaaccgt atcgacaact
     5701 atgaagtggt gggtaaatcg cgccccagtc tgccagagcg catcgacaat gtgctggtct
     5761 gcccgaacag caactgtatc agccatgccg aaccggtttc ttccagcttt gccgtgcgaa
     5821 aacgcgccaa tgacatcgcg ctcaaatgca aatactgtga aaaagagttt tcccataatg
     5881 tggtgctggc caattaattg cggttggtaa taaaagtctg gctccctata atgagccaga
     5941 ctttttaccg ctgtaataaa ggagaaatca tgagcaaaac tatcgcgacg gaaaatgcac
     6001 cggcagctat cggtccttac gtacagggcg ttgatctggg caatatgatc atcacctccg
     6061 gtcaaatccc ggtaaatccg aaaacgggcg aagtaccggc agacgtcgct gcacaggcac
     6121 gtcagtcgct ggataacgta aaagcgatcg tcgaagccgc aggcctgaaa gtgggcgaca
     6181 tcgttaaaac caccgtgttt gtgaaagatc tgaacgactt cgcaaccgtt aatgctacct
     6241 acgaagcctt cttcaccgaa cacaatgcca ccttcccggc acgttcttgc gttgaagttg
     6301 cccgtctgcc gaaagacgtg aaaattgaga tcgaagcgat cgctgttcgt cgctaatctt
     6361 gatggaaatc cgggctatca tgcccggatt aagtctgatg acaaacacaa aatcgcctga
     6421 tgcgctacgc ttatcaggcc tacgtgattc ctgcaattta ttgaatttgt tggccggata
     6481 aggcatttac gccgcatccg gcatgaacaa aactcacttt gtctgcaatt cgaatccagg
     6541 ccaccatgcc cagttgattc tttattgcca gccgtaacga cggctataaa atcctttcac
     6601 caactgggtt aatgtcatat accctgccag aatcgcaacc agccacggga aatagcttaa
     6661 cggcagcgcc tgcagttgca gataactggc cagcggtgaa aacggcaatg cgatcccgac
     6721 aatcatcacg atcacggtca tgatcattaa cggccacgat gcacagctct gaataaacgg
     6781 cacacggcgg gtgcggatca tatgcacaat cagcgtttgc gacagtaagc ccaccacaaa
     6841 ccatcccgac tggaacagcg tttgcgtttc cggcgtgttg gcatggaata cccaccacat
     6901 caggcaaaac gtcagaatat cgaagatcga gctgatcggt ccgaagaaga tcataaagcg
     6961 ccccagatcc gccggattcc aacgctgcgg cttttgaatt tgctcgtcgt cgacgttatc
     7021 aaacgggatc gccacctgtg acacatcata cagcaggttc tgaataagca agtgtaacgg
     7081 caacatcggc aggaagggca agaaagcact cgccaccagc acgctgaaca cattaccgaa
     7141 gttggagctc gccgtcattt tgatgtattt cagcatgttg gcgaaagtgc gacgtccttc
     7201 aataaccccc tcttccagca ccatcaggct tttttccagc aggatgatat cagccgcttc
     7261 acgggcaata tctaccgcgc cgtccacaga aatgccgata tccgcagcgc gtaaggcggg
     7321 cgcatcatta ataccatcgc ccataaagcc aaccacatgc ccttcgcgct tcagtaaggt
     7381 cacaatgcgt tctttatgca tcggcgtcag gcgggcaaac agcgtggtgc gctgtgcgag
     7441 atttgccagt tcgtcgtcag atagcgtttc aatatcacta ccaatgacca cctctcccgc
     7501 gtccagcccc acttcatggc acactttcgc tgcaactaac tcactgtcgc cagtaaggat
     7561 ttttacggtg attccactcg cttttaatgc cttcagtgcc ggagcggttg tctcttttgg
     7621 cggatcaagg aaagcaatat atccttcgag gatcaggtcg gattcatccg cccgctggta
     7681 atctccttca cgcgctggca ggtatttcgt cgccaccgca accacgcgca gcccctgacg
     7741 attaagcgta tcagtaaccc gcttaatctt acgcagcatg atgtcatcga gcggcacaat
     7801 ctcgccattg tgacgcacct gcgaacacac attgaggatt tcctgcaatg cacctttgca
     7861 aaccagctga tggtgctcgg tattttctgc cactaccacc gacatccggc gacgctcgaa
     7921 atcaaacgga atctcatcaa ttttttgcca acgactggcc agcgagcgcg ctgactcttc
     7981 atccgtacct tcgagcaccg ctgtatcgag caggttttta agtccggtct gataatgact
     8041 gttcaaccac gcgctatgca gcacgcgttc gctggtttta ccggagatat cggtatgatt
     8101 ctccagcaca attttgtcct gtgtcagggt gccagtttta tcagtgcaca gaatatccat
     8161 tgcgccaaag ttctgaatag catccagatg tttgacgatc actttctgtt tcgacagctt
     8221 tactgcgccg cgcgccagcg tcgaggtaac aatcatcggc aacatttccg gcgttaagcc
     8281 taccgcgacc gaaagcgcaa acagtgccgc ttcccaccag tcgcctttgg tgtaaccatt
     8341 gattaacagc accaccggag ccatcaccag cataaagcga atcagcagca tactgacgcg
     8401 gctgatccct tgctgaaagg cattcggctc gctttcctgc tcactaacac gctccgccag
     8461 ttgaccaaac caggtattgg cacctgtagc aatcaccatt gcttgtgccg tgccgctcac
     8521 cacggtggtg cccataaaac acagggtgtc acactccagc ggattgctgt gctccggctg
     8581 gcgagtggta gcggcttttt ctacgggcag agactcaccg gttaacgacg cctgagcgac
     8641 gaacagatcc cgcgcctgca agatacgtaa atctgccggg atcatatccc ccgccgccag
     8701 ttttattata tcgccgggca ccagctggtc gatcgggatc tccagccagc cattttcgcc
     8761 tttgtcgtta attacgcgca gaaccgtcgc tgtattgctg accatcgctt tcagagcatc
     8821 tgccgcttta gtggaacgtg cttcctgaat aaagttcagc aacgtagaaa tagcgaccat
     8881 tagcgcgata acgcccgcgg caaataaatc ttccgtggcg taagaaatag cgccgagaat
     8941 ggtgagtaaa atattaaagg ggttgcgata gcagacccat aaatgtaccc accacggcga
     9001 cggttgttgt gcgggtaatt tattttcacc atgttgttcg cgggcagatt ccacttccgc
     9061 ctgatttaac ccttccggat gggtgtcgaa cgttttccat aattcttctt cgggcatcac
     9121 cgccattttc aggcaatgcg cacttaagga cggcgggacc gccgtattca ctgtctgctg
     9181 cgcgccaggc aatggatcgc gatgaaccag acgggaaggt aaatggcgaa tgagccgggt
     9241 aaaaatttct ttaaacataa ggagtccctc cgcactgtct gaaacagtac agaaatgtct
     9301 tcaggcgtgg cgaagcctta cctgaaggta ataaagctga gcagggacgt taaaacgtca
     9361 cggccttacg catccggtaa cgccgtgaaa agacgggctt accggaaaag ttttaatctc
     9421 cgtcgaggga gaggcgtggg atcaggttcc atataacctc cggtaagtga aaaaagcgcc
     9481 gcaaataata aggagaatcc aataatattc gtagcatcta tggcgatata atacctgctg
     9541 gcaactaaac caacgataaa ccagacttta cccattgctg aatgcacggg taaagttagg
     9601 ctcaaataat taaacaacac gttacaggac aacaggatgc aaaatcggct gaccatcaaa
     9661 gacatcgcac gcttaagcgg cgtggggaaa tctacagttt cccgggtgct gaataacgaa
     9721 agcggcgtga gccagcgcac ccgcgagcgt gttgaagcag tgatgaatca gcatggattt
     9781 tccccttccc gctctgcgcg cgctatgcgt gggcaaagcg ataaagtggt cgccatcatt
     9841 gttacccgcc tggattcgtt gtcagaaaat ctcgccgttc aaaccatgct gccagcgttc
     9901 tatgaacaag gttacgaccc aatcatgatg gaaagtcagt tttccccgca attagttgcc
     9961 gaacatttgg gggtgctgaa acggcgtaat atcgacggcg tagtgctgtt cggttttact
    10021 ggcataacag aagaaatgtt agcccactgg cagtcatcgc tggttctgct ggcgcgtgac
    10081 gcaaaaggtt ttgcttcggt ctgttatgac gacgaagggg caatcaagat cctgatgcaa
    10141 cggctgtatg accaggggca tcgtaatatc agttatctcg gcgtgccgca tagtgacgtg
    10201 acgaccggga agcgacgtca cgaagcctac ctggcgttct gcaaagcgca taaactgaat
    10261 cccgttgccg ctctgccagg ccttgctatg aagcaaggct atgagaacgt agcaaaagtg
    10321 attacgcctg aaactaccgc cttactgtgc gcaaccgaca cgctggcact tggcgcaagt
    10381 aaatacctgc aagagcaacg catcgacacc ttgcaactgg cgagcgtcgg taatacgcca
    10441 ttaatgaaat tccttcatcc ggagatcgtc accgtagatc ctggttacgc cgaagctgga
    10501 cgccaggcgg cctgccaatt gatcgcacag gtaaccgggc gcagcgaacc gcaacaaatc
    10561 atcatccccg ccaccctgtc ctgatcgttt cctgaacgat aaattgtgat cttcgctgcg
    10621 cttcgggaac gttcccgttt ttaaattttt ccgcgcaata tattctgcag ccaaccaaaa
    10681 atgtcatctg ccatggggct ttatgatgag caaaataaac caaacggata tcgatcggtt
    10741 gattgaactg gtcggcgggc gcggcaatat tgcgacggtg agccactgta ttactcgcct
    10801 gcgctttgtc ctcaaccaac cggccaatgc cagaccgaaa gaaattgagc aactccccat
    10861 ggtgaaaggc tgtttcacca atgccgggca atttcaggtg gtgattggca ccaacgtggg
    10921 tgattactat caagcactaa tagcgtcaac cggacaggcg caggttgata aagagcaggt
    10981 aaaaaaagcc gcccggcaga atatgaaatg gcatgagcag ttgatctctc atttcgcgga
    11041 gatcttcttc ccgttgctgc ccgcgttgat tagcggcggt ttgatcctcg gttttcgtaa
    11101 tgtgatcggc gatttgccca tgagcaacgg tcaaacgctg gcgcaaatgt acccttccct
    11161 gcaaacgatc tacgattttc tgtggttgat cggtgaagcg atcttcttct acctgccggt
    11221 cgggatttgc tggtcagcgg tgaaaaaaat gggcggcacg ccgatccttg gtatcgtgct
    11281 tggcgtgaca ctggtttccc cccagctgat gaacgcttat ctgctcgggc agcagctgcc
    11341 ggaagtgtgg gactttggca tgttcagcat cgccaaagtg ggctatcagg cgcaggtgat
    11401 cccggcactg ttagccgggc tggcgctggg cgttattgaa actcgcctta aacgcatcgt
    11461 accggattac ctctatctgg tggtggtgcc cgtctgttcg ctgatcctcg cggtgttcct
    11521 cgcccatgcg ctgattggtc cgtttggtcg catgattggc gatggcgttg cctttgcggt
    11581 acgtcacctg atgaccggca gctttgctcc gattggtgcg gcattgtttg gcttcctgta
    11641 cgcgccgctg gtgatcaccg gcgtacacca gaccaccctt gctattgatt tgcagatgat
    11701 tcaaagcatg ggtggcacgc cagtgtggcc gctgattgcg ctgtcgaata tcgctcaggg
    11761 ctccgccgtg ataggcatta tcatttccag ccgcaagcac aatgaacgcg agatctccgt
    11821 gcctgccgct atctccgcct ggcttggggt cactgagcct gcaatgtacg gcatcaacct
    11881 gaaatatcgc ttcccgatgc tgtgcgcgat gattggttct ggtctggcag gattactatg
    11941 cggcctgaac ggcgttatgg cgaatggtat cggcgtaggc ggcctgccgg gaattctctc
    12001 gattcaaccg agctactggc aggtatttgc gctggcaatg gttatcgcca tcatcatccc
    12061 gattgtactc acctcgttta tctatcagcg gaaataccgc ctgggcacgc tggatattgt
    12121 ttaattttct tcggggcgca attgcgcccc ctcgcatttg caggaataac gtaatgacta
    12181 atcttcccca ctggtggcaa aacggcgtta tctaccagat ttatccaaag agttttcagg
    12241 acaccacggg tagcggcacc ggcgatttac gtggcgttat ccaacgcctg gactatctgc
    12301 ataaactggg cgttgatgcc atctggctaa ccccctttta tatctctccc caggtcgata
    12361 acggttacga cgtagcgaac tatacggcga ttgatcccac ctacggcacg ctggacgatt
    12421 ttgacgaact ggtgacgcag gcaaaatcgc gcgggattcg tatcattctc gatatggtgt
    12481 ttaaccatac ctctacccaa catgcctggt ttcgcgaggc gctgaacaaa gaaagccctt
    12541 accgccagtt ttatatctgg cgcgatggcg aaccagaaac gccaccgaac aactggcgtt
    12601 caaaatttgg cggtagtgcg tggcgctggc atgcggaaag cgaacagtac tatttgcatc
    12661 tctttgcacc agaacaggcg gatctcaact gggagaatcc agcggtacgc gcagagctga
    12721 aaaaagtctg tgaattctgg gccgatcgtg gggtcgacgg gttgcgcctg gatgtggtga
    12781 atctgatctc caaagacccg cgtttccctg aagacctgga tggcgacggg cgtcgctttt
    12841 acaccgacgg gccacgagca cacgagtttt tgcacgagat gaaccgcgat gtgtttacgc
    12901 cacgcgggtt aatgaccgta ggtgaaatgt cctccaccag ccttgagcat tgccagcgat
    12961 acgcggcact gacaggcagt gaattgtcga tgacctttaa ttttcatcac ctgaaggtcg
    13021 attatcccgg tggtgaaaaa tggacgctgg ctaaacctga ctttgtggcg ttgaaaacat
    13081 tgttccgcca ctggcaacaa ggaatgcaca acgtggcatg gaatgccttg ttctggtgta
    13141 accacgatca gccgcgcatt atttctcgct ttggtgatga aggtgaatac cgcgtgcctg
    13201 cggcaaaaat gctggcgatg gtgctgcatg gcatgcaggg aacgccgtat atctaccagg
    13261 gcgaagagat tggcatgacc aacccgcatt tcacgcgcat tactgactat cgcgacgtgg
    13321 agagcctcaa tatgtttgcc gagctgcgca acgatggtcg tgatgccgac gagttattgg
    13381 caatcctcgc cagtaaatcc cgtgacaaca gccgcacgcc catgcaatgg agcaacggcg
    13441 ataatgccgg gtttacggct ggcgaaccgt ggattggcct gggcgataac tatcaacaaa
    13501 tcaacgtaga agccgcgctg gccgatgact cctcggtgtt ttacacctac caaaagttaa
    13561 tcgcactgcg taagcaagaa gccatcctga catggggcaa ttaccaggat ctgctgccaa
    13621 acagccctgt attgtggtgc tatcgccgtg aatggaaggg gcaaaccttg ctggtcattg
    13681 ccaaccttag ccgtgagatc caaccctggc agccagggca aatgcgcggc aactggcagc
    13741 ttgtgatgca taactacgaa gaagcctcac cacaaccctg tgccatgact ttacggcctt
    13801 ttgaggctgt ctggtggtta cagaagtaag tatccgctaa gccccgggaa tgcctgtcat
    13861 tccggggttt tgtctgtcag catgttgttt ttgttgattt ttcaaccagc aaattcatta
    13921 aaaaatttac ataacgctgt agcgcccgtc atccgtacgc tctgcttttt actttgagct
    13981 acatcaaaaa aagttcaaac atccttgatg caaagcacta tatatagact ttaaaatgcg
    14041 tcccaaccca atatgttgta ttaatcgact ataattgcta ctacagctcc ccacgaaaaa
    14101 ggtgcggcgt tgtggataag cggatggcga ttgcggaaag caccggaaaa cgaaacgaaa
    14161 aaaccggaaa acgcctttcc caatttctgt ggataacctg ttcttaaaaa tatggagcga
    14221 tcatgacacc gcatgtgatg aaacgagacg gctgcaaagt gccgtttaaa tcagagcgca
    14281 tcaaagaagc gattctgcgt gcagctaaag cagcggaagt cgatgatgcc gattattgcg
    14341 ccactgttgc cgcggttgtc agcgagcaga tgcagggccg caaccaggtg gatatcaacg
    14401 agatccagac cgcagttgaa aaccagctga tgtcgggtcc atacaaacaa ctggctcgtg
    14461 cttacatcga gtaccgtcac gatcgcgaca ttgaacgtga aaaacgcggt cgcctgaacc
    14521 aggagatccg tggtctggtc gagcagacca acgcctcgtt actcaacgaa aacgccaaca
    14581 aagacagcaa ggtgattcca acccagcgcg acctgctggc cgggatcgtg gctaaacact
    14641 atgcacgcca gcacctgctg ccgcgtgacg tggtgcaggc acatgagcgt ggcgatattc
    14701 actatcacga tctcgattac tcgccgttct tcccgatgtt caactgcatg ttgatcgacc
    14761 tgaaaggcat gctgacccag ggctttaaaa tggggaacgc cgagattgaa ccgccgaagt
    14821 cgatctctac ggcaaccgcg gtaactgcgc agattattgc tcaggtcgcc agccatattt
    14881 atggcggcac caccattaac cgtatcgatg aagtgctggc gccgtttgtt accgccagct
    14941 acaacaaaca tcgcaagacc gcagaagagt ggaacatccc ggacgccgaa ggctacgcaa
    15001 actctcgtac catcaaagag tgctacgatg ccttccagtc gctggagtac gaagtaaaca
    15061 ccctgcacac cgccaacggt cagacgccgt ttgtaacctt tggttttggt ctgggcacca
    15121 gctgggaatc gcgcctgatt caggaatcga tcctgcgtaa ccgtatcgca ggcctcggta
    15181 aaaaccgtaa aactgcggtg ttcccgaaac tggtgtttgc gattcgcgat ggtctgaacc
    15241 ataaaaaagg cgatccgaac tacgacatca aacagctggc gctggagtgc gcaagcaagc
    15301 gcatgtatcc ggatatcctg aactacgatc aggtagtgaa agtcaccggt tcgtttaaaa
    15361 ccccgatggg ctgccgcagc ttcctcggcg tgtgggaaaa tgaaaacggc gagcagatcc
    15421 acgatggtcg taacaacctc ggcgtgatca gcctgaacct gccgcgtatt gctctggaag
    15481 caaaaggcga tgaagccacc ttctggaagc tgctggatga acgtctggtg ctggcacgta
    15541 aggcgctgat gacccgtatc gctcgtctcg aaggcgtgaa agcgcgcgtg gccccgatcc
    15601 tctatatgga aggtgcttgt ggcgtacgtc tgaatgctga cgatgatgtt tctgaaatct
    15661 tcaaaaacgg tcgtgcgtct atttcgctgg gttacatcgg catccacgaa accattaacg
    15721 cgctgttcgg cggcgagcat gtttacgaca acgagcagct tcgcgcgaaa ggtatcgcca
    15781 ttgttgaacg tctgcgccag gcagtggatc agtggaaaga agaaacgggt tatggtttca
    15841 gtctctacag cacgccgagt gaaaacctgt gcgaccgctt ctgccgtctc gatactgctg
    15901 agtttggcgt ggtgccgggc gtgaccgata aaggttacta caccaacagt ttccacctcg
    15961 atgtggagaa gaaggtgaac ccgtacgaca agatcgactt tgaagcgcct tacccgccgc
    16021 tggcgaacgg tggtttcatt tgctacggcg agtatccaaa cattcagcac aacctgaagg
    16081 cgctggaaga tgtctgggat tacagctatc agcatgtacc gtattacggc accaatacac
    16141 cgattgatga gtgctacgag tgtggcttta ccggtgagtt cgagtgcacc agcaaaggct
    16201 tcacttgccc gaaatgtggt aaccatgacg cctcccgtgt gtcggtaact cgccgcgtgt
    16261 gcggatattt aggtagcccg gatgcacgtc cgtttaacgc tggtaagcag gaagaagtta
    16321 agcgccgcgt taaacatttg gggaatgggc agataggtta atcttcactc tgcgctaagc
    16381 aaatgccgga tgcggcgtaa acgccttatc cggcctacat ttggcaacgg ctgtaggcct
    16441 gataagacgc gacagcgtcg catcaggcat tgattaccgg atgcagccta cacgcggact
    16501 gataagccag gcgaaataat gaattatcat cagtactatc ctgtcgacat cgtcaacggc
    16561 cccggcactc gctgcaccct gtttgtctcc gggtgtgttc atgaatgccc cggttgctat
    16621 aacaaaagca catggcgggt aaattctggt cagccattta ccaaagcaat ggaagacaag
    16681 atcattaaag atctgaatga cactcgtatc aaacgccagg ggatttccct ctccggcggc
    16741 gatccgctgc atccgcaaaa cgtgccggat attctgaaac tggtaaaacg catccgcgcc
    16801 gagtgtccgg gtaaagacat ctgggtgtgg acaggctata aactcgacga actcaacgct
    16861 gcgcaaatgc aggttgttga tttgattaac gtgctggtcg acggcaaatt tgtgcaggat
    16921 ttaaaagacc cttccctgat ctggcgcggc agcagcaatc aggtggtgca tcatttgcga
    16981 tgataagttc cctgttacct gagtgcaggc aacagggaaa tgaggaatta acgatacttc
    17041 tggtgatagg cgttgcgggt cgttttcagt tgctctgcag cagcctgcgc ttcttttact
    17101 ttaccttcat ttgccagctt cagcgcgtcg tcaatctgac cgaccagaat gtcgaaaccg
    17161 tggcggaaat ctttcatttc cgggctgtcc ggtgatttat cttcgagctt cggcggcgtt
    17221 gctttttgcg catccagcgc tgcggcgcgc atcatcgtta acgcgtcttt gacttgcgcc
    17281 gcgttatccg ctttttcgat cacttttaaa ttgtcgttga gggtttccat attgtcttca
    17341 agatcagcgg caaacgacgc agaactgaat accaacgagg agactgcaag aatagctaac
    17401 aggcttttac gcattgctca cttccttttt tatttacact tcatccttca cactgcctca
    17461 tcgacggcta cgctcattcg cccgggtcac gtactgcgag tacgctcccc ggccctcatt
    17521 cactttccgt ctcgatgcag tgcgaatgat tttgtgtaat tttttagttt atagcgcggc
    17581 aggtcgcgcc agttttttac tgcccggcga ttttcatctc cggcaacagc acagaaccac
    17641 actgtatatt actgcgtgtt tcaatatcgt taccgacggt gacaatattg cgccacatat
    17701 cttttaaatt acctgcgatg gtgatttcac taacgggata ctgaatttcg ccattctcta
    17761 cccagaagcc cgctgcacca cgggaataat caccggtaat ggcactcacg ccctggccca
    17821 tcagctccgt caccaccagc ccggtgccca tctctttgag catttgttcg aagcttagcc
    17881 cttgtccggc aatccgccag ttgtgaatac cgcccgcatg tccggtgctt ttcagcccca
    17941 gtttccgcgc cgagtagctg gtcagcagcc actgagtcag gatgccatct ttaataatat
    18001 cgcgacgctc ggtgcgcaca ccttcgctgt cgaatggcgt cgacgccagc cctttcagca
    18061 gatgcggatg ctcttcaatg gtcagccagt ccggcagaat ttgtttaccc agcgaatcca
    18121 gcagaaaggt agatttacgg taaaccgagc cacccgctat cgcccccacc agatggccaa
    18181 aaagcccggt tgccacttca ttggcaaaaa tgactggcgc tttcatggtg gagagtttac
    18241 gcggtgacag gcgcgataaa gtacggcgag cacagtcggc cccaacccac tctggcgttt
    18301 gcagatcgct catcgcacga ccaatggtgt aggcgtaatc acgctccata tcgccatttt
    18361 cttcggcaat tacacagctg gagagtgaat gacgcgtaga gcagtaaccc tgcaacatgc
    18421 cgtggctgtt gccaaaaact ttgacaccgt agtggctgtt aaaactgccg ccttcggtat
    18481 tggtaatgcg tttgtccgcc tgcaatgccg cctgttctgc gcgggccgcc agttcaatgg
    18541 cttcatccgg ggaaacttcc gcagggtgga acaagtcgag atctggtgcg tcaaaggcca
    18601 gcagctcttt gtctgccacg ccggcacagg gatctggcga ggtataacgg gcaatatcca
    18661 gcgccgcctg tacggtgcgg gcaatggcct gcgggcttaa atcggtggat gatgcgctac
    18721 ctttgcggtt ctggtgataa acagtgatcc ccagcgcgcc atcgctattg aattcaacat
    18781 tctccacttc accataacgc gtgcttacgc taatgccggt ggtcttgctg acggcaactt
    18841 ccgcaccgtc cgatttgcct gaggccaact ccagcgcagt ggaaactgct tcttccagaa
    18901 tcttgcgctg cgcttcaact tgagagatta ctttcattgc aagtgccata atgtagagag
    18961 tttctctgaa gtctaacaga gaaccgtttt tcagtgcgca tcttaactgg taacattagc
    19021 ctcttttttt aaggagcctg agatgactaa gcagcccgaa gactggctcg acgacgttcc
    19081 cggtgatgac atcgaagacg aagacgatga aattatctgg gtcagtaaaa gtgaaattaa
    19141 acgtgatgcc gaggagctaa aacgccttgg cgcggaaatt gttgatctgg ggaaaaacgc
    19201 gctggataag atcccgttag atgcggatct gcgcgcggct attgagctcg cccagcgtat
    19261 taagatggaa ggtcgccgcc gccagttgca gctcatcggt aagatgctgc gccagcgcga
    19321 cgtagagcct attcgtcagg cgctggataa gctgaaaaac cgtcacaacc agcaggtggt
    19381 gctgttccat aaactggaaa acctgcgcga tcgtctgatc gatcaaggtg atgacgccat
    19441 cgctgaagta ttgaatctgt ggcccgatgc cgatcgtcag cagctgcgta ctctgatccg
    19501 taacgcgaag aaagagaaag aagggaataa gccgccgaaa tccgcacgcc agattttcca
    19561 gtatctgcgc gaactggcgg agaatgaagg gtaattttcg ttgttgatgt gagtatggtt
    19621 tgccggatgc ggtgcacctg taggccggat aagacgcatc aagcgtcgca tcaggcacta
    19681 tgctcaaatg ccggatgcgg cgtaaacgcc ttatccggca atgctatctc aggctgaggc
    19741 cgaattactg cgcagcttcc gccttcttcg ccagaccatc cagcagtttc tgatggatcc
    19801 caccaaaacc gccgttgctc atcaccagaa tatggtcgcc aggctgagcg gttttcacca
    19861 ccatatctgc cagcgtatcc acatcgccac tccagtgtgc aggctgaacg caggcttctg
    19921 ccacttctgc cacctgccac ggaatatgcg ccggttgcag caggaagact tcatcggcac
    19981 gacctaatga aggtgccaga tcgtctttgc agatccccat tttcatggta ttcgagcgcg
    20041 gttccagcac agcaataatg cgcgccgtac caccaacttt gccacgcagc gccgccagcg
    20101 ttgccagaat cgccgtcggg tgatgggcaa aatcgtcata taccgtgacg ccattcgctt
    20161 caccacgcaa ctccagacgg cgacgagcat taataaacga acccagcgcg ttagcggcat
    20221 ctgccggcgc tacaccaaca tggcgagccg ctgcaatcgc catcaggcca ttgtgcatat
    20281 tatgttcgcc taccagcgac catttcactt cgcccacttt ttcgccatcc agcaagactt
    20341 cccattcgga agcatcggtg gtcagctttt tcgcctgcca gtgcccctgc tcacccacca
    20401 gctcctgctc gctccagcag cccatcgcca tggtctgttt caggttgatg tcattttccg
    20461 gccagataat acggccctgc cccggaacga tacgcaccag atggtggaac tgtttctgga
    20521 tcgctttcag gtcgtcaaag atatcggcgt gatcgaactc aaggttgttg aggatcagcg
    20581 tacgcgggca gtaatggaca aatttagagc gtttgtcgaa gaaggcgcag tcatactcat
    20641 ccgcttcgat aacaaagaag tcgctttcgc ccagacgcgc cgaaacctca aagttccccg
    20701 gcacaccgcc gatcacaaat cccggtttgt aaccgcactg ttccagaatc caggtcgcca
    20761 ttcccgcggt ggtggttttg ccatgtgtac cggcaacggc cagcacccag cggtcacgca
    20821 gcacaaaatc gtgcagccac tgtggacctg acatataagg gatgtttttt tccagtaccg
    20881 cttccacaca cggatttcca cgggtcatgg cgttgccaat aatcaccaga tccggctgcg
    20941 gctcgagctg gctggcatcg taaccctgaa tcagctcaat gccttgcttc tcaagtaagg
    21001 tgctcatcgg cggatacaca ttggcgtccg aacccgttac ttcatggcct aactggcgcg
    21061 ccagcatcgc cagaccgccc ataaacgtgc cacaaattcc taaaatatga atgcgcatac
    21121 gtcactatcc ttttttaatc tgccgctcat tttacgcata tgtcgggcaa gtgagaaacg
    21181 catttcagga taatccgtaa tttgctggcg cgattcacct gagcgcaaca ttgagattat
    21241 ttgttaagat tgttgcggtc gctttactcc ataaacattg cagggaaagt tttatgaaaa
    21301 cgttaggtga atttattgtc gaaaagcagc acgagttttc tcatgctacc ggtgagctca
    21361 ctgctttgct gtcggcaata aaactgggcg ccaagattat ccatcgcgat atcaacaaag
    21421 caggactggt tgatatcctg ggtgccagcg gtgctgagaa cgtgcagggc gaggttcagc
    21481 aaaaactcga cttgttcgct aatgaaaaac tgaaagccgc actgaaagca cgcgatatcg
    21541 ttgcaggcat tgcctctgaa gaagaagatg agattgttgt ctttgaaggc tgtgaacatg
    21601 caaaatacgt ggtgctgatg gacccactgg atggctcgtc caacatcgat gttaacgtct
    21661 ctgtcggtac cattttctcc atctaccgcc gagttacgcc cgtcggcacg ccggtaacgg
    21721 aagaagattt cctccagcct ggtaacaaac aggttgcggc aggttacgtg gtatacggct
    21781 cctctaccat gctggtttac accaccggat gcggtgttca cgcctttact tacgatcctt
    21841 cgctgggcgt tttctgcctg tgccaggaac ggatgcgctt cccggagaaa ggcaaaacct
    21901 actccatcaa cgaaggaaac tacattaagt ttccgaacgg ggtgaagaag tacattaaat
    21961 tctgccagga agaagataaa tccaccaacc gcccgtatac ctcacgttat atcggttcac
    22021 tggttgcgga tttccaccgt aacctgctga aaggcggtat ttatctctac ccaagcaccg
    22081 ccagccaccc ggacggcaaa ctgcgtttgc tttatgagtg caacccgatg gcattcctgg
    22141 cggaacaagc gggcggtaaa gcgagcgatg gcaaagagcg tattctggat atcatcccgg
    22201 aaactctgca ccagcgccgt tcgttctttg tcggcaacga ccatatggtt gaagatgtcg
    22261 aacgctttat ccgtgagttc ccggacgcgt aatctcctgc gcctctccac acggagaggc
    22321 gtttttattg ctgcgcaatg ttgactcttg tcactggcga actctgacga ttctcccaca
    22381 gcaccgttaa tccacgctgt aatgctataa aaataaacaa caaaataccg atggcgattt
    22441 tcgtccacca ggaactcagc gtgccatcaa agtttatgta agtctgaatc agtccctgaa
    22501 ttgccacgcc aaaaagcgtc ccaagcaccg tcccgacgcc accacttaac aacgtaccgc
    22561 caatcaccac cgacgcgatg gcgtccagtt ccacacctac gcccgccagt gcatatccgg
    22621 cctgggtata aatcgagaag acaatccccg ccagtgttgc cagtccggtg gagagcatat
    22681 aaatgcgaat agtggtgctg cgagtagaaa tccccatcag gttcgccgac gtcgcgttgc
    22741 cgccaatagc gtacacctga ttgccaaaac gggtacgatg cgcaaggaaa ataccgataa
    22801 ccaccaccgc cagcatcaac agccccatcg cactaaggcg accgccgccg gggattttcc
    22861 acgcaaggct tgagagcgtg tcataaatgg gatggtttat cggaatcgac tcttccgaaa
    22921 cgagatagct gacgccgcgc aaaaagaaca tccccgcaag cgtaatgata aatgccggga
    22981 tcttcagggc gtcgatcaga agccccataa atgcgccgaa ggcacagccc atcaccagca
    23041 ctagcggaaa cgccagcagc ggcgagaggc cgaaatcgcc aatcactttt gccagaaaca
    23101 cgccagtaaa ggcgatcacc gaaccgacgg agagatcgat accgccagag aggatcacaa
    23161 aggtcatgcc aacggcgatt atcccaagaa aggcgttatc tgtcaggata ttgcagatca
    23221 ctcttgtgga agcaaaaccg ggaaactgcg tcaggcagta gaaatagccc agcacaaaga
    23281 cgccgatggt gatcatcagt ggcaaattac gttttatcat ggctacgtac tcctttaatc
    23341 agactgataa agcgttgcga ctgaacaatc agcacacaaa gcaccaccac cgcttttacg
    23401 acctggttca tctccggcgg aaagccagaa agtaaaattc cggtgttcat cccctgaata
    23461 atcagcgccc ccacgactga gagcagcagg ttaaagcgcc cgcccatcag cgatccgccg
    23521 ccaatcacca ccgcaagaat ggcgtccagc tccagccata acccggcgtt attggcatcg
    23581 gcaccgcgaa tatccgccgc cacgataatg cccgcaatcg ccgcacacag cccgctcaac
    23641 acgtaggtga gcatgacgat gatccgcgtg tttaccccgg cattttttgc cgcccgaatg
    23701 ttgataccaa cggcttcgat aaacatcccc agcgccgttt tgcgggtcaa cagccagaac
    23761 aggagaagcg tcagcaccgc gataatgacc ggcgttggca ggaacaacag cgatccgctg
    23821 ccaaaccatg agagatccgg cgagttaaac gtgacgatct gcccggcggt gatcagttgc
    23881 gccacgccgc gcccggcgac catcaggatc agggtggcga caaacggctg aattttgagg
    23941 atcgctacca gtatgccgtt ccacaatccc gccaggatgc cggtgcccag ggcgcttaac
    24001 aaaacaatcg gcaggctgaa tcccgcgacc gtcatcgcag ccgttgtggc tccggcgata
    24061 gccatcaccg cccctacgga gagatcaatc ccaccggtgg cgatcaccag cgtcatacca
    24121 atcgccagta acgcaacggg agctgcacgg ttaagaatgt cgatagggct accgaacaaa
    24181 cgcccatcct gaagcaccac ctgccagaaa tgcggggcta ccaggctatc gaccagcagc
    24241 accagcaata gcgccgccag ctgcggcatt cccgttggcc agcgaaagcg ccttttcggc
    24301 ggcgtagtgt ccgggagaga ttgaggcatc acactgttct ccttacgccg caatggcgtt
    24361 catgatcgcc ggaacggaaa gctctgccag cgggatctcc gccacctgtt tgcgatcgcg
    24421 catgataatc acccgatcgg catagcccac cagctcttcc agttcggagg agatcaccaa
    24481 cagcgccaga ccgtcagcac acagcgtttc aatcaggcgg atgatctcgg cgtgggcacc
    24541 aacatcaatg ccgcgggttg gctcgtcgag gatcagaaat tgcggtcggg tcagtagcca
    24601 acgtgaaagc aacacttttt gctgattgcc gccagagaga aattcaatcg gttgttcagt
    24661 tgaaggtgtg cgaatgccaa gctggcggat aaagcgttcg gcaatctctt gctgctcttt
    24721 gcgggaaatg ggtcgtagcc agccgcgctg ggcctggaga gcgaggatga tattttcccg
    24781 taccgaggcg gcggcgatga tgccatcggt tttcctgtct tccgggcaga agccgatgcc
    24841 cagtaccgaa gcctgatgtg gcgatcgcag gttttgctgt ttgcctttga tcaacgccgt
    24901 gccgctgtca gcaggtttga taccgaagat cacttcggcg gtttcggtac gtccggatcc
    24961 cagcaagcct gccaggccga cgatctcgcc ggggcgtact tcgagatcaa acggtgcgat
    25021 cgttcctttt ttgccgtaat ttttgaacgc ggcaacgggt ttgtcgctca acaatgttcg
    25081 cccggcacgc tgtagcgcgt gggtatccag ctcgcgcccc agcatcattt ttaccagttc
    25141 gatctgcggt agctcgcgcg tttcccgaca gcctacgaaa ctgccgttgc gtaagacggt
    25201 gatccgatcg ctgacctgat agacctgatc gagaaagtga gtgacaaaga tcaggctgac
    25261 gccgcgatcg cgcaactgac gcatcaggcc aaacagtaac tctacttcct gggtgtcgag
    25321 gctggcggtg ggttcatcga ggatcagcac tttggcggag agatcgatag cccggcaaat
    25381 ggcgacgatt tgctgcatcg ccaccgaaaa acggttaagc ggttcacgca cgtcgaggga
    25441 gaaaccgtaa gatgtcatca gttcggtggc acgcttttcc atctctttgc ggcgtagaaa
    25501 gccgaaacgt ttgggttcgc gacctatgaa tagattatca gcgaccgaca tattgggtag
    25561 caggttgact tcctgataaa cggttccgat gccgagttgt tgtgcatggg cggtattttt
    25621 cggtgagata gcctggcctt ccagccagat ggtgccgcga tcggcgtggt atacaccggt
    25681 taatgctttg attagcgtcg attttcccgc cccgttttca ccgagcagcg ccatgatttc
    25741 gccacggcgc aggctgaaat caacgttgtc taacgctttg acgccgggga aaaatttact
    25801 taatccttcg gtgcggagga tttcctggtg ttggtcggtc gtcatggttt cccccttagc
    25861 cacgctctaa aagtctggga atagatcgtg cctgatgtta agagctgcgc cgtttgtccc
    25921 cctcacccta accctctccc cgaaggggcg aggggacaga ttgtgcacgg tgttaagtcc
    25981 ggtgttgcac cactgccgga tgcggcgtga acgccttatc cggcctacac agagcaccga
    26041 actcgtaggc atgataagac gcggtaagcg tcgcatcagg cttcagcgca ccaccgccgg
    26101 atgcggcgcg agcgccttat ccggcctacg aatcggcatc aggcattgat accctcaata
    26161 ccccatattt ttcttcttct ctaactcttc ttttgcggta tcaggcagat aaagggtgga
    26221 tttggtcagc gttagctttt caggcatggt gccgtctttt ttgtatttct ccagcgcgtc
    26281 gaaggcggga cctgccatat tcggcgtcag ttcaacactg gcgttcgctt cgccatcaat
    26341 catcgctttg tagatatccg gtacgccatc aatggaaccc gtgaggatat ctttacccgg
    26401 cttcaggccc gcttctttaa ttgcctgaat tgcaccaatc accatgtcat cgttatgggc
    26461 gtaaaccatg cagatgtttt tgccgttgtt ttccgctttg ataaagctct ccatgacttc
    26521 tttgccttta ctgcgggtga agtcacctga ctgcgagcgg ataattttga tatttggcgc
    26581 attcttaatg gcttcggcaa agcctttctt acggtcaatg gcgacgctgg ccccaacggt
    26641 gccctgcagc tccaccacgt tgcatggttt gccattcact tcttttacca gccagtcacc
    26701 aatcaacttg ccttcgagga tgttgtcggc ggtgacagtg gtcatataga gagatttgtc
    26761 tttcacatcg atggaacggt caagcaagaa gaccgggatt tcggcatctt tcgcctcttt
    26821 taataccggc tcccaacccg tcgctaccac tggagcgata aagatcgcat ccaccccttg
    26881 tgcaacgaag gaacgtaccg ctttaatctg gttttcctgc ttttgttgac catcggcaat
    26941 tttcaacgta attccgcgtt tttcggcctc acttttcgcc acattggttt cagcggcacg
    27001 ccagccggat tccgatccga cctgcgaaaa tcctacggtt aacggagcgg ccaacgccat
    27061 agacgacatg gctgccgaga ctgcagagac tacaagtaag cgtttccaca tagggttgtc
    27121 ctcgtcgggg taattattat tggtgaaaag atgttcgcga aaaaactata gacaattcgt
    27181 tatgtaacgg attgcgttac atcacacttc aaaaaagtaa ataaaacgtt aatcacaagt
    27241 ttgtaatcgc tttcatctca ctatgaaaaa tgcggctacg gttatggatt ttcctgctct
    27301 gcataccgtc ttaaaactgg cgaaaaagga aaatgaagac gaaaacaagc gaagacattc
    27361 ggcgcgagtt ggctataata ctcggcactt gtttgccaca tatttttaaa ggaaacagac
    27421 atgagcttac tcaacgtccc tgcgggtaaa gatctgccgg aagacatcta cgtggttatt
    27481 gagatcccgg ctaacgcaga tccgatcaaa tacgaaatcg acaaagagag cggcgcactg
    27541 ttcgttgacc gcttcatgtc caccgcgatg ttctatccgt gcaactacgg ttacatcaac
    27601 cacaccctgt ctctggacgg tgacccggtt gacgtactgg tcccgactcc gtacccgctg
    27661 cagccgggtt ctgtgatccg ttgccgtccg gttggcgttc tgaaaatgac cgacgaagcc
    27721 ggtgaagatg cgaaactgat tgctgttccg cacaccaagc tgagcaaaga atacgatcac
    27781 attaaagacg ttaacgatct gccagagctg ctgaaagcgc agatcgctca cttcttcgag
    27841 cactacaaag acctcgaaaa aggcaagtgg gtgaaagttg aaggttggga aaacgcagaa
    27901 gccgctaaag ctgaaatcgt tgcctccttc gagcgcgcaa agaataaata agttcttctg
    27961 gcgcaataac cctgaacacc gggcttcggt tagtaagggt ttttttatgc ccgcgataaa
    28021 taaacacacc ttattccacc accgcctgca agcgtaataa cgcctcttcc accacctcat
    28081 ccgcagccag accaatacgc tttgccagcc gggcgcgtag atccatcatc cgcacctgat
    28141 tcaccagcac cacgccgtgc acatcgcctt cttcgcaatg taaaggaacg ctaaatccgg
    28201 cataacgggc aaaatttccg ccctgcgtaa tgggggccac cagcgtcatt cccagttgat
    28261 taaaggcttg aacggagagc acaagcgcag gtcgaccagc accttgctgt tcatggccgc
    28321 ttgctggatc aaagccaacc agcacaatgt ctccccgttc aaattcactt ttctttacca
    28381 tatttcgtca cccgcagggg tggatttacc ccagacatcc tgctcgctaa gttccgcggc
    28441 gttcatgtca cactgtgcca gcagttcatc aagcgagtag cgcctggaga tgggtgtcag
    28501 aatcagttga ttgttgctca cttgcgcctc cacgctctgc cccggctgta agttaagttc
    28561 tttcattacg atattgggaa tgaccatacc tgcactgttc ccccatcttt ttatggtaat
    28621 acgcatctgc acctccttaa gttatacaaa gtataactca cggtgctaca ggaaacgttt
    28681 tacccctgcg catttgtgaa cgccagcaga gttttttatg cgttaacaga tatggaaagc
    28741 ggctcggaga gaaaagcaaa gggtgaggga attacaaaac agaatgcgag tcgtctcgca
    28801 aaaaacaacg ccacccgaag gtggcgtatg catatggtta cttatccctg tctaaccagt
    28861 cgccgctttc aattagcttt aatccatcga cgggtcgttg ataaacgtac atccatgcac
    28921 tcccgtacgg cgtctgaatc aactggcgcg cgtattcacc gcccctggtg cgcaaggcat
    28981 caagttcggc cagcgtggcg ttgtcaatac gataaacttc accgtgtact gttccgttcc
    29041 ccggaactgc gcctggatag tggcccaggc tatacaactg gtagttatcg atactgaaat
    29101 cgcccagtaa ctgggcattg gtcatccagt gactgttgcc ttgtttgtgg cgtaaactgc
    29161 cgtagacaaa tattcgcatt gctaaaactc gaactgatag agcaaatcca gtgcctggtc
    29221 tacaccagac acggcttcca gatatagctt aggcatcagg cgataacgta acgtgagtgt
    29281 tgctatagag tcaaatatac ccacgccgta tttcacttgc agacctggca atacatagcc
    29341 gctgactact acctgggagg agtcgcctac tccctgggtg tcgagcgcta aattgcttac
    29401 gccaaacgtc tcgccgattt tacccacaat ctggccactt tgcgcaaccc ccagaccaat
    29461 cagcatcgag gtcattgccg cactgtcgct ctgatcgctc tccagccctt gtccacgtag
    29521 caaataagac aatgcagctt gttgcgacat cgccgggtca gagaagatct ccgctttcgg
    29581 ttcgtccgcc agaccagtga cgcgaactcc ggcgattacg tcgtcttctg tagcatccgg
    29641 gttacgaata gcttcaatat taagatacgg ttgatccggc ggaccagaga acagtaactc
    29701 acccttacgc acaatcagat cctgaccata ggcatggaag cgcccttcag ggatgttgat
    29761 ctgcccgttc aggcccagcc cttgtttgtc ctgtacgacg ttaagatcgc ccgtcagccg
    29821 cgctttcagg ccaaaggcgt caatgcgcac attgttgcca acgtggacaa tcaggttact
    29881 gttaatcgga atcgacgccg ttttcggctc ttccggttgc aggttatcgt taagcatcac
    29941 cacatcgctg gagacgccta ctgcgctttc cggcagatcg tgcaccacga tgcgcgccca
    30001 cgggacatcc acgcgaccat cgagggtaaa caggtttggt gtagcctcga atacaacatc
    30061 tggcgataca tccattcgta ccatcggcgg cacggtgatc cgcactttac tgcctttcgc
    30121 cgttactcgt gcccgccagt tttcaatttg gctccagtcg gcatcaccgt tcaggtagat
    30181 ttcaccctgc tgggtccgta ctgtaccggc aagcgtcgag cgcataccgt taaagttgac
    30241 cgcaagctgg ctcggctgca tatcaaacgg cataaagttg ccgtcgatat ccacacccgt
    30301 aacctgaagc tgaccaaaca actgcgggct ttgcacatca ccacccagac gcaagttggc
    30361 actcaccatc cccgctgctt tttccccacg ggtaaagatg gggtttatca tcgcaaggtt
    30421 gaagttacgg atattgacgt tgccaccaag attacggcgg ccttgcggat cggtcacctg
    30481 cacctgtcca tcaaactggc cgttattggt caggcggatg gtccagccca attcggcacg
    30541 gttgttacgc aattccgccg tcagattcag tgtctgaaac gccaccggca gcgccgcatc
    30601 gttgacggtt tgcgttacct gcacgttacg ccccgaaagg gtgatactgc cctgcggcag
    30661 cccctctttc gtggtgtccc aggcaacatc cgctttaccc gtgaagatac cgctggcctg
    30721 agtggtttgc ggcataaacg gtttcagcat ggcgaggtcg aagcggttga gattcaccac
    30781 cgcacgccct tcggcccccg catcgatagt ttgcggcaca cacagctccg cattcgggtt
    30841 aagccaacag tgtggcccga tgctgatttt ttgctcctta ttgcggtaat ccagcgcaat
    30901 atcgcgggtc agcgaccacg ggccgaccgg cgtctggaag cgggtattgc taagaattcc
    30961 cttccagcgt tcttctttgc gatcaaaact tcctgccaga ttaagctgcc cggagacagg
    31021 ctcgccctga atccgcaact gtagctcgtg ctgcttttcg ctgcctttgg cattcagggt
    31081 gacgaggttg atatttacat ccggctgcga aatttgctca acgcgtacgt cgagtttccc
    31141 ggcgatctga tcggtggatt taatgtcgcc ttccacgcga acctgcgcca cggaaagttc
    31201 ctgccagcgc aggccgcgcg cggtgatatc cgccagtagt tgtggcgctt ccaccgtgcc
    31261 gcgtactttc accagccctt tcgctgtacc gccaagcccc ggcagcgcgt tatccagccc
    31321 cggcgcgttg atggtggcat caagattgag atcttttacc cccagctcgc ctttcacttc
    31381 ggcactgttt ggccccagtt ccagatgaag ccctgggatc atccactgca tataactgtt
    31441 gcctttcagc gtgccgtcaa cgttcacttt gttctgtttg acgttaccgg tcagcttcag
    31501 ttctggcacc tccatctgcc aggtgccgcc gtacaggcta ccgcgggttt taatcaagcc
    31561 attgagtttc gacggccact ctgggatctc tttggcggtg ttaatgccgt taagcgttag
    31621 ctcaccgcgc caactaatgg cctgctgcca gtcgagcaac gccttgagtt cagttttccc
    31681 ttccagcgcc gcgacggtga gtttgtcgag attgacctgc tgttcattac ctttggcatc
    31741 aagggtaatg gtggctggcg ggatctccag tcccttcact gccgtacgca tagagagcgt
    31801 gtaatcggtc attttgccgg taagtttcag tttcagatca tccgcctgat actgcttctc
    31861 accggtgaac ggccagtaaa gctgtttgct gttcacttcc acgttgagcg gcaatccggc
    31921 ttccgccagt cgcgtctggg cgcgtaaatc catatccacc ggaccggaaa ggttaacgcc
    31981 aatctccagc tgttcgcgca gcgcaccgcc cactttcagc ttcagttttt ctcctttcaa
    32041 cggctccacg tttagtgtgc tgttgagggt gatatccacc ggccagttgt ctgacagctg
    32101 cgccgtgccg ctggcgttga cgatcccttg actggaatcg atatccaggg cgtccagttt
    32161 agtattgccg tcaatgctgc tcactttcag cagcatggtg cgcacggtga tgtccgtgtc
    32221 gcccgtcacg cgtaactgct cgcctttaaa ctcttcaatg ttcaggttaa gcggcaaatg
    32281 cacgtcggtc atttccggca ataccgggcg agaaaagaga tctttcagcg tttcgccgag
    32341 cggcttttcc tccggctgcg gattttcaat tttcggttcg accacttctt cctgcgccac
    32401 ttccgccact ttcggcagag caatcagcag gcctttcagc gacgtcggtt tcagggtcag
    32461 ggttttctcc tgccagttca ggccggaggt gaagtccatc accgacaccg tggtgtcatc
    32521 aatcttgatg ttgacgttgt ccagcgccac ccgtgtcagg gtgatgggat acggcgtgga
    32581 gagatccagc ggaccgctat cttcctcttc ttcaacctgt tcagaaggag gcattttttt
    32641 actgtcgatg ttgacctgaa tgtctttcag cgccaggtca ttaatacaaa cgctactgtt
    32701 ccacaggcac tcaagcccga ccgccagatg cagattgccc gcttttaccg ccacgcctgg
    32761 ctgctcataa cgaacgtcag acaaggtgag atcgcgccag ccgccggtga ccttgccaat
    32821 atccagtcct ggcacccagc gatccgccgc tttaaatacc agatgcaggc cgctggtggt
    32881 gcccaccaga aacgccaccg atcccagcaa cagtaagata acgataacca cgccgaggct
    32941 gatttttttc cataaactca taattctggc cccagaccga tgtaaaactg taacccgtgc
    33001 tcgtctttat ccgcgaccgg tacggcaaaa tcgagtttga ttggcccgac cggcgattcc
    33061 cagcgtacgc cgaccccggt accggtttta aagtcgctgc ggcgaatatc gcttaccgct
    33121 tcgccactat cgacaaacac cgcgccccac cattttccgg tcacgttgta ctggtattcc
    33181 agcgatccgg ttatcagctt cgaggcccct ttcaggtcac cgttggcgta tttcggggcg
    33241 atagatttgt atttgtagcc gcgaatactg cgatcgcccc cggcgaagaa acgcagatcc
    33301 ggcggtactt tgtcgaaatc acccgtttca atccagccca gcgtgccgcg tgtcacaaaa
    33361 cgatggcgat cgtacagtgt gcggatccag acgttctgcg cctggaaaac ggagaaatcg
    33421 acatctgagc cccaggccgt gttggagtag tcgatagagt agcgttgcga gtcgccccag
    33481 gttggcatca ggccaccacg agaacgcgtg cggctaatca tcaccccagg ataaaacagc
    33541 atcgtggtgt tggtaatttc accctgggta aagtggtcga ggctccagcg caggttaatg
    33601 gcacgctgcc agccgctgga gagatcccag tagcgagaag ccaccagcgt agtggagtca
    33661 gattcggtat cgttcaggtc agtgcgctta aaaccgccct gcaccaaata atattgttcc
    33721 agtggattct tcagcagcgg cattttatag ctgaagtcga gggtctgttc cggcgcggaa
    33781 atactggtac tggtggtcag actgtgacca taagagttca tccacggctt tttccacgtc
    33841 gctttcacgc gcggtcccac gtccgtagag taaccgaccc cggtttcgat ggtgttttca
    33901 gttcgcggcg aaaccacgcc cgtcaagggt aatactttcg tttcgcgcgc tttatcaaat
    33961 tgtggagcca ccaccaccga gttaaaccag ccggtagcag aaagtcggcg gttcagttcc
    34021 gccagatctt tcgattcgta ctcatcgccc tctttaaacg gcaccagatt ttgcaggtat
    34081 tcatcgcgga tttgtgatcc ttcaaaggtc acatgcccaa agcggtaacg ttcgccactg
    34141 ttataatcaa tatcccagaa ggctttatgc aggccgagcg caatgcccag ctgcgctttg
    34201 gtaaattcgc tatcgaaata acctttacgc aacgcaatgc tggttaagga ctttttgaaa
    34261 ttttcataat cgccctggtt cagcaccgtg ccaatagccg ggcgagtatc gagcaatttc
    34321 aaatagtctt tatcggtccg cgcgccgccg cgcaatacca catcggtgcc gccaattaac
    34381 accggcacac ctggcgtgac tttggcgatc aatacctgcc gccctttctt tggcggtgga
    34441 cggagatcaa attcaatggt cggctggtaa taacccagcg ctttcagacc ttcacggatg
    34501 gcatcatcga cgcgtgcgcg aaagcgacgg tctggcgtca cttcatcact ttcaatcgta
    34561 gaaagctgcg cacgaacgtt cttttccagc tgtcccgata acccctcgac ctgtagacgg
    34621 acgttcgcgg cgacggcaga tccgcttaag cagagtaagc ttacacagca taactgtcgg
    34681 atatagcgca cattttctcc tgaatatcct tttttcctgc ccctggaacg ccgttaaacg
    34741 gcttaacaaa aatccagtaa tatggattaa aaagcagact aaaccccaaa tatttcttat
    34801 gttttacttt agacctattc acggtggtta ttgtgtgcaa atacgcctct tgttacaacc
    34861 ttaaccccaa tgaccgattt tcgggagagc gacaccatga gtttatttga taaaaagcat
    34921 ctggtttccc ccgccgatgc cctgcctgga cgtaacaccc cgatgcccgt agccacgctg
    34981 catgcggtca acggtcactc aatgaccaat gtacctgacg gaatggagat tgccattttt
    35041 gcgatgggtt gtttctgggg tgtggagcgt ctgttctggc agttacccgg cgtttacagc
    35101 accgccgcag gctataccgg aggctatacg ccaaatccga cttatcggga agtgtgctcc
    35161 ggtgatacgg gtcatgccga agcggtacgc attgtttacg atccttccgt catcagctat
    35221 gagcagttgc tacaggtatt ttgggagaat cacgatcccg cccagggaat gcgtcagggc
    35281 aatgaccacg gcacgcagta tcgttcagcg atttatccgc tgaccccaga acaggatgcc
    35341 gcagctcgcg ccagtctgga acgttttcag gcggcgatgc ttgccgccga tgatgatcgt
    35401 cacatcacca cggaaatcgc taacgccaca ccgttttatt atgccgaaga tgaccaccag
    35461 caatatctgc ataaaaaccc gtatggttac tgtggaattg gcggaattgg cgtctgtctg
    35521 ccaccggaag catagcgtta cgggtacaaa tgtagattgt tgataaagtg cgctttattt
    35581 atgccagatg cggcgtaaac gccttaccag gcctacaaaa tcgtccaaat tcaatatatt
    35641 gcaaggactg cgtaggcctg attggcatag cgcatcaggc agtattgcat ttatcagcag
    35701 tctgaatttt aaccctctgg cgactttaca gtaccttacg ctatactagc cactgaaaat
    35761 gccggttcac tttcttcgaa tcggctttca atgtgtattt cacacaaatt aatcaacttc
    35821 cccttccgag gatctggcct gaaaggtcgg ataagatatg ttaaacagta ttttagtcat
    35881 actctgtttg atcgctgtaa gtgcgttctt ctcgatgtcc gagatctcgc ttgccgcctc
    35941 acgcaaaatc aaacttaaac tgctggctga tgaaggcaat ataaatgccc aacgcgttct
    36001 gaatatgcag gaaaatcccg gcatgttctt taccgtggtc caaatcggtc tgaacgcagt
    36061 ggcgattctc ggcggtatcg tcggtgatgc ggcattttct ccagcttttc acagcctgtt
    36121 ctcccgctat atgtcggcag aactctctga gcaactgagc tttattctct ctttctcgtt
    36181 agtgactggc atgtttatcc tgtttgcgga tttaaccccg aaacgcatcg gtatgattgc
    36241 gccagaagcg gtggctttgc gtatcatcaa cccgatgcgc ttctgcctgt acgtttgcac
    36301 cccgctggtg tggttcttca acggactggc gaacataatc ttccgtattt tcaaactgcc
    36361 aatggtacgt aaagatgaca tcacttctga tgacatctac gcggtagtgg aagccggtgc
    36421 gctggcaggc gtgttacgta aacaggaaca cgagctgatt gaaaacgtct ttgagctgga
    36481 atcccgtacc gttccgtctt caatgacacc gcgtgaaaac gtgatttggt ttgatctcca
    36541 cgaagatgag caaagcctga agaataaggt ggcggaacat ccgcactcta agttcctcgt
    36601 ctgtaatgaa gatattgacc acatcatcgg ttatgtcgat tctaaagacc tgctgaaccg
    36661 cgtgctggct aaccaaagcc tggcactgaa cagcggcgta caaattcgca acacgctgat
    36721 tgtgccggat acgttaaccc tttcagaggc gttggaaagt tttaaaaccg caggtgaaga
    36781 cttcgcggtg atcatgaacg agtacgcgct ggtggtgggg atcatcaccc tcaacgacgt
    36841 gatgaccacg ctgatgggcg atctggttgg tcaggggctg gaagagcaga ttgtcgcccg
    36901 tgatgagaac tcatggctga ttgacggcgg cactccgatt gacgacgtca tgcgcgtgct
    36961 ggatattgac gagttcccgc agtcgggcaa ctacgaaacc atcggcggct ttatgatgtt
    37021 tatgctgcgt aagatcccga aacgcaccga ttcggtgaaa ttcgccggct acaaatttga
    37081 agtggtggat atcgataact accgcatcga ccagctgctg gtgacccgga tcgacagcaa
    37141 ggccaccgcc ctttcgccaa aactgcctga cgctaaagat aaagaagaaa gcgtcgcgta
    37201 gcccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc atagcacttt
    37261 ggttaagcca tctctgtttg cagacgcata acctgacggt taacttcgga catcactgac
    37321 aaatgcagct tatccttcac ttttgggata agaatcttac ccttatcaaa ttcaaaagcg
    37381 ccaacgtcct tgatgtataa ccgtccacgg aacaggatct ttacgtactt cgccacctga
    37441 agtgggttgt accgttggaa aattttcatt gttatctcct gctgagtatt acgcctgtgc
    37501 ggtgccacaa tcggcccaac tattatgagg cgcaaatttt aatgcttagt gactatagac
    37561 tatccgggca atgtttccac cgcgtataac tttttttacc taaaggttac aattattcag
    37621 aattatcttt ttaccgaagc gcggtcttca gtataagcat tcatttttca tatgatttgt
    37681 gcgcttgacc gcaaactggc atcacacttg cgggaaattc gataaatagc acatatgatt
    37741 aaaactcaga cccaagtggt cggatcacct gcatatcata agaaggaaac accatgaccc
    37801 tacgcaagat tctggcactc acctgcctgc tgttgccgat gatggcttcc gcacatcagt
    37861 tcgaaaccgg tcagcgagtg ccgccgattg gcatcaccga tcgtggcgag ttggtgcttg
    37921 ataaagatca gtttagctac aaaacctgga acagcgcgca gttagtggga aaagtgcgag
    37981 tactgcaaca tattgctggt cgcacctctg caaaagagaa aaacgcgacg ctgattgaag
    38041 cgattaaatc agcgaagtta ccgcacgatc gttaccagac caccaccatt gttaacaccg
    38101 acgacgcaat tccgggttcc ggcatgtttg tgcgcagcag tctggagagt aataaaaagc
    38161 tttatccctg gtcgcagttt attgttgata gcaatggcgt cgcacgcggt gcctggcagc
    38221 tggatgaaaa aagttccgct gtagtggtgc tggataaaga cggtcgcgtg caatgggcca
    38281 aagacggggc gcttactcag gaagaggtgc agcaagtgat ggacctgctg cataaattaa
    38341 ttaataaata gacactctga acccaggatt caggaacgac tcgcgcggag tgtaatccag
    38401 cggtttaccc tgccagtcgt gaacgtgcgc tccggcagcc gcagccacag catgaccagc
    38461 ggcggtatcc caaatattcg ttggcccgaa acgcgggtac agctgcgcct gtccttccgc
    38521 caccaggcag aatttcagcg aagagccgat ggacgtggtc tgatgttcgc caagctgttg
    38581 cagatactct ttcagctccg catccgcatg ggaacggctg atcaccacca gcggcgggcg
    38641 cgcatcgcgg acctgaatct gcttgcgcac gccgcactct tctttccacg ctttgccttc
    38701 tgccgcgctg tacattacgt tcattaccgg cgcatacacc acacctaaaa tcggtttgcc
    38761 atggtcaatg agtgcaatgt taacggtgaa ttcgccatta cgtttaataa actctttcgt
    38821 accatccagc gggtctacca gccagtaacg ctgccagtgc tgacgggctt cccaaccggg
    38881 aggatcttct tcagaaagga ccggaatatc cggtgtcagc gtacgtaaac cgtccatgat
    38941 aacggtgtgt gcggcaatat ccgctgccgt taccggagaa ttgtccgctt tgctgacgac
    39001 gtccatcggt ttcgtcccgt cgtagacctg cataatggca tcgcctgcat tccgtgcaag
    39061 ctggcatact tgatctaaca tttctccacc tcgtctctgt gagcggtgtt aacttattgt
    39121 tttacttata ccctatcgtt aatgaatgcg ccaactgtga tagtgtcatc attttcaaag
    39181 cgtaaaattg tggcattctt cactgttcta taagtaagac gtttattctc ccttttcttt
    39241 cgtattcccg atgataaaag gatgtccctg atgattaagt ttagcgcaac gctcctggcc
    39301 acgctgattg ccgccagtgt gaatgcagcg acggtcgatc tgcgtatcat ggaaaccact
    39361 gatctgcata gcaacatgat ggatttcgat tattacaaag acaccgccac ggaaaaattc
    39421 ggactggtac gtacggcaag cctgattaac gatgcccgca atgaagttaa aaacagcgta
    39481 ctggtcgata acggcgattt gattcagggg agtccgctgg ccgattacat gtcggcgaaa
    39541 ggattaaaag caggtgatat tcacccggtc tataaggcat taaatacgct ggactatacc
    39601 gtcggaacgc ttggcaacca cgagtttaac tacggtctgg attacctgaa aaatgcgctg
    39661 gcaagagcga aattccctta tgtaaatgcc aacgtcattg acgccagaac caaacagcca
    39721 atgtttacac cgtatttaat taaagacacc gaagtggtcg ataaagacgg aaaaaaacag
    39781 acgctgaaga ttggctatat tggcgtcgtg ccgccacaaa tcatgggctg ggataaagct
    39841 aatttatccg ggaaagtgac ggtgaatgat attaccgaaa ccgtgcgcaa atacgtgcct
    39901 gaaatgcgcg agaaaggtgc cgatgttgtt gtcgttctgg cacattccgg gctgtctgcc
    39961 gatccgtata aagtgatggc ggaaaactca gtttattacc tcagtgaaat tccgggcgtt
    40021 aacgccatta tgtttggcca tgctcacgcc gttttcccgg gtaaagattt tgctgatatc
    40081 gaaggggctg atatcaccaa aggcacgctg aatggtgttc cggcggtaat gccgggcatg
    40141 tggggcgatc atcttggggt ggtcgactta caactcagta atgacagcgg taaatggcag
    40201 gtgacgcagg cgaaagcgga agcacggccg atttacgaca tcgccaataa aaaatccctc
    40261 gcggcggaag acagcaagct ggtagaaaca ctcaaagccg atcacgatgc cacacgccag
    40321 ttcgtcagca agccaatcgg taaatctgcc gacaacatgt atagctatct ggcgctggtg
    40381 caggacgatc cgaccgtgca agtagtgaac aacgcgcaaa aagcgtatgt cgaacattac
    40441 attcagggcg atccggatct ggcaaaactg ccggtgcttt cagctgccgc accgtttaaa
    40501 gttggtggtc gcaaaaatga cccggcaagc tatgtggagg tggaaaaagg ccagttgacc
    40561 ttccgtaatg ccgccgatct ttatctctat cccaatacgc tgattgtggt gaaagccagc
    40621 ggtaaagagg tgaaagagtg gctggagtgc tccgccggac agtttaacca gattgatccc
    40681 aacagcacga aaccacagtc actcatcaac tgggacggtt tccgcaccta taactttgat
    40741 gttattgatg gtgtgaatta tcagattgat gttacccagc ccgcccgtta tgacggcgag
    40801 tgccagatga ttaatgccaa tgcggaaagg attaagaacc tgacctttaa tggcaagccg
    40861 attgatccga acgctatgtt cctcgttgcc accaataact atcgcgctta cggcggcaaa
    40921 tttgccggga cgggcgacag ccatatcgct tttgcttcac cggatgagaa ccgctcggtt
    40981 ctggcagcgt ggattgctga tgagtcgaaa cgtgcggggg aaattcaccc ggcggcagat
    41041 aacaactggc gtttagcacc gatagctgcc gataagaaac tggatatccg tttcgaaact
    41101 tccccgtcag ataaagccgc agcgtttatt aaagagaaag ggcagtatcc gatgaataaa
    41161 gtcgcgaccg atgatatcgg gtttgcgatt tatcaggtgg atttgagtaa gtaaaacact
    41221 tctttttcgg cctataaatc atcaatcgca tccggcattt attggcggat gcgatgctgg
    41281 cgcatcttat ccgccctaca agtcatacac cgtaggccgc gcatccggca gttatgccgc
    41341 acgttcatcc cgcaccgcca acacctcggg caaattcaac tcaatccagt ccgccagtgc
    41401 agcaaccttt tcgctcacct gctcgcccag cggcgtgagg ctgtattcaa catgcggtgg
    41461 caccaccgga tacgcgatac ggttaagaaa accatcctgt tctaacgcct gtaacgactg
    41521 cgcaagcatc ttttcgctca ccccgccgat tttgcgccgc aggtcgctaa agcgatgagt
    41581 accttcgcgt agcgccacca gaatcaaccc cccccaacgg ctggtgacgt gtttcaacac
    41641 ctcgcgcgac gggcactgtt ccgcaaagag attgccctct ttcagttgtt gcgacaggct
    41701 aacctgactc atttcatact tacctttttg tatgtactta ctaaaagtaa gtttaggtgt
    41761 tagcgtattt aaacacaaga caaaacgatg gagacttccc atgatcgcta ttactggtgc
    41821 cactggccaa cttggtcact atgttattga atccttgatg aaaacggttc ctgccagcca
    41881 aatagtggct atcgttcgta atccggcaaa agcccaggcc ctggcagcac aaggcattac
    41941 cgtgcgtcag gctgactacg gcgatgaagc cgcactgaca tctgcacttc agggagtgga
    42001 aaaactactg ctgatatctt ccagcgaagt gggtcaacgt gccccgcagc atcgtaatgt
    42061 tattaatgcc gcaaagacgg ctggcgtgaa atttatcgct tataccagcc tgctacatgc
    42121 agatacctcc ccgctcggcc tcgccgatga gcacattgag acggagaaaa tgttggctga
    42181 ttctggcatc gtttacaccc tgctgcgcaa cggctggtac agcgaaaact acctcgccag
    42241 cgccccggca gcactggaac acggcgtatt tatcggtgcg gcgggcgatg gcaaaatcgc
    42301 ctcagcaacg cgggcagatt atgcggcagc tgcggcacgc gtgattagcg aagtcggtca
    42361 cgaaggcaag gtttacgaac tggcgggcga tagtgcctgg acgttgacac agttagcggc
    42421 agagctgacc aaacagagcg gaaaatccgt tacctatcaa aatctgagcg aagccgattt
    42481 cgccgcggca ctgaaaagcg tcggactgcc cgacggactg gcggatatgc tggcggattc
    42541 tgacgttggc gcatcgaaag gcggtctgtt tgatgacagc aaaacgctta gcaaactgat
    42601 tggccaccca acgacaacgt tagccgaaag cgtaagccat ctttttaatg ttaataacta
    42661 gttaattaaa gtggcatcct cccgcatcct ctctgataat gacgggatgc cgggagcaat
    42721 catgtctgct tcctgaactt tcttctgaca gatcaatgga tgccagtaat gattagcggc
    42781 gtgctgtacg ccctgttagc agggttgatg tgggggctta tttttgtcgg gccgttgatc
    42841 gtgccggaat acccggcgat gttgcagtcg atggggcgtt atctggcgtt agggttaatt
    42901 gcgctgccca ttgcctggct gggacgcgtg cgtctgcgtc agttggcgcg tcgcgactgg
    42961 cttaccgcct tgatgctcac tatgatgggc aacctcattt attacttctg ccttgccagt
    43021 gccattcaac gtactggcgc gcctgtttcc acgatgatta tcggcaccct gccggtggtc
    43081 attcctgtct ttgccaatct gctttatagc cagcgcgacg gcaaactcgc gtggggaaaa
    43141 ctcgccccgg cactgatttg tattggcatc ggcctcgcga gtgtgaatat tgctgagtta
    43201 aaccacggac tccccgattt tgactgggca cgttatacct ctggcatcgt gctagcgtta
    43261 gtttccgtgg tctgctgggc gtggtatgcc ctgcgcaacg cccgctggct gcgggaaaat
    43321 cccgacaaac atccgatgat gtgggcaaca gcgcaggcgc tggtcacact gccggtttca
    43381 cttatcggct atctcgtcgc ttgttactgg ctgaatacgc aaacgccgga cttctcccta
    43441 ccctttggcc cccgtccgct ggtgtttatt agtctgatga ttgcgatagc cgtgctttgc
    43501 tcatgggttg gcgcactctg ctggaacgtc gccagccagc gattaccgac agtgattctc
    43561 gggccgctga ttgttttcga aacgctggca ggtttgctgt acaccttttt actccgccag
    43621 caaatgccgc cgctaatgac gctgagcggt atcgcgctgt tagtgattgg cgtggtcatc
    43681 gcggtcagag caaaaccaga aaaaccttta actgaatctg tctcagaaag ttgacacgct
    43741 ggcagtgagt taaataagcc tctgctacgt aagggttata gcttttgcct taaagatgca
    43801 tttaaaatac atcttatctt attaagaatg aggtatcagc tatggcttat cgcgaccaac
    43861 ctttaggtga actggcgctc tctattcctc gcgcttcagc tctgtttcgt aaatatgata
    43921 tggattactg ctgtggcggt aagcagacgc tggcgcgcgc ggcggcacgt aaagaactgg
    43981 atgttgaggt cattgaagct gaactggcaa agctcgctga acaaccgatt gagaaagact
    44041 ggcgtagcgc cccgctggca gaaatcattg accatatcat cgtgcgctac cacgatcgtc
    44101 accgcgagca gttgccggag ctgatcctgc aagcgactaa agtcgagcgc gttcacgccg
    44161 acaaaccgag cgtgccaaaa gggctgacaa aatacctgac tatgctgcat gaagagcttt
    44221 ccagccacat gatgaaagaa gagcagatcc tcttcccgat gatcaaacaa ggcatgggca
    44281 gccaggcaat ggggccaatc agcgtaatgg aaagcgagca cgatgaagcg ggtgaactgc
    44341 tggaagtaat caaacacact accaataacg tcacaccgcc gccggaagcc tgcaccacct
    44401 ggaaagcgat gtataacggc attaatgaac tgattgatga cctgatggat cacatcagcc
    44461 tggaaaacaa tgtactgttc ccacgcgcgc tggcgggtga gtgatctaaa agctggatgg
    44521 cattgcgcca tccagcatga taatgcggtt atttccgcag ttcagcagcc cgcttcttac
    44581 caataaacag ccagcccaac cccagcgcga taaaccacag cggggtaacc agcagcgcct
    44641 ggcgagtgtc atcttccagt gtcagcaaca cgaccacgaa cacaaagaac gccatacata
    44701 cccagcacat cagcttgccg agcggcatct tgtagatcga cttctcatgc agatgaggac
    44761 gctgtttgcg gtacaccagg tacgagcaaa ggataattgt ccagacgaac ataaacagaa
    44821 tcgcggaaac ggttgtaatc atcgtgaacg cgccaatcac gctaggattc acatacaaca
    44881 tcaccacgcc gccgagcaga cagatacacg agaaggtcaa ccctttcgcg ggtactgcgc
    44941 gcttagaaag tttagcgaac gctttcggtg ctacaccttc ctgcgccaga ccaaacagca
    45001 tacggctggt agagaagacg ccgctgttag cggaagacgc cgcagaggtc agcaccacaa
    45061 agttgatcac gcttgcggca gcaggcagcc ctaccaacac gaacagttca acaaacgggc
    45121 ttttctccgg gactaccgaa ctccacggcg tcacggacat aattacaatc agcgcgaaga
    45181 cgtagaacat aatgatacgg atcggaatag agttaattgc acgcggcagt gatttctccg
    45241 gatctttggt ttccgcagcg gttgtaccta ccagctcaat ccctacgaaa gcgaaaaccg
    45301 ctatctggaa tccggcaaag aagccactta aacctttcgg gaaccagccg ccatcattcc
    45361 acaaatgcgc gaatgacgct tccacaccag tcggtgactg aaagtgcatc gccaccataa
    45421 ccaggccgac gacaatcagc gacacaatgg cgacgatttt gatcatcgca aaccagaact
    45481 ccatctcacc gaacattttc acggtggcga gattgagcgt cagcagcagc actatcaccg
    45541 ccagcgaggc gacccagtcg gagagatcgg ggaaccagaa ctgagcataa gcagtgatcg
    45601 ccaccacgtc tgccataccg gttacaaccc agcagaacca gtaagtccag ccggtgaaat
    45661 atcctgccca cggcccgagt aaatcggaag cgaagtcact aaaagatttg tattccagat
    45721 tcgaaagcag caattccccc attgcccgca tcacgaaaaa gagcataaaa ccgatgatca
    45781 tataaacgaa aatgatcgac ggcccggcaa ggctaatcgt tttgccagac cccataaaca
    45841 accccgtacc aatggcaccg ccaatggcaa taagctgaat atgtcggttt gtgagattgc
    45901 gccgtagcga ctgttcagcc ggagcctgat catcggcaac gacttttacc tgatctacca
    45961 tgtttttttc ttcctgtacc tgtctgtgtt gttcaggctc tacggccttt agtcagtcta
    46021 tgatacgacg aatctgtatc gggactcatc gatattaggt aacaatacgc gggatgaata
    46081 ctaagattta gatctaatgt taattttatg tttaaagtga gtgtcatatc acggtgataa
    46141 cgcgaaacag ctcacaaaaa tacacgctta cggggtcttt gcaacacaaa atagcgccgt
    46201 ttggcaaact gaagggttta ttgctgaatg cctgctcccc tctggttcga ggggaacaga
    46261 atgcgctgct tagaggattt ccagcagttc gacttcaaac accagggtgc tgaacggagg
    46321 gatggatgcg cctgcgccgc gctcgccata tgccagttcc tgcgggatag tcagttccca
    46381 tttagaacct accggcatca gagtcagtgc ttcaatccag ccagggatca cgccattaac
    46441 cggaaattca gcgggttcac cacgagcaac ggagctgtca aacacggtgc cgtcgatcag
    46501 tttaccggtg taatgaacac gaacgcggtc ggtacgtgcc ggaattgcgc cttcaccctg
    46561 gttgatcacg cggaattgca ggccagattc ggtgctattc acaccttctt ttttggcgtt
    46621 ttcttccagg tatttcacac cttcagcagc catcgcctgg aaacgctgac gacgaacggc
    46681 atcggcgcgt tcgtggattt cacgcagcgc gcgatgcacc acatcaaccg gaacagccgg
    46741 atgtttgcct tccagcgcat cggcaatacc tgcaaccagt gcttctggca gcagcccttc
    46801 caggccagat tcactcagtt gttgccctac ctgcaaacca atgccgtagc ttgcttgcgc
    46861 ttcgatggtg tcaaaagttg gggtggtcat cgtatttcct ttcttagggt ataaaagtag
    46921 cgggcagcat atcagccaca cttctttggg taaaactttg tcgcaggaat gatgacaaat
    46981 cccggggaaa aagaaacaat agcaatatcc cgtatgaata taataaaggc gggtttacca
    47041 cggacatcat agcgttagct tattcatagg ctatgattga ggaacaagac gcggagcagg
    47101 aggaaaccat gcccgggcgc tttgaactaa aaccaaccct ggagaaagtc tggcacgcgc
    47161 cggacaattt tcgcttcatg gacccgctgc cgccgatgca tcgtcggggg atcattattg
    47221 ccgccattgt attggtggtc ggatttctgc ttccatctga tgatacgccc aacgcgccgg
    47281 tggtgacgcg tgaagcgcag ttagatattc agtcgcaatc acaaccgccg acagaagaac
    47341 agctgcgggc gcaattagtc accccacaaa atgatcctga tcaggtggct ccggtcgctc
    47401 cagaacctat acaggaaggc cagccagaag agcagccgca aactacccaa acgcaaccat
    47461 tccagccaga tagcggaatt gataatcaat ggcgctctta ccgtgtcgaa cccggtaaaa
    47521 cgatggcgca gctgttccgc gaccacggac tgcccgctac cgatgtctat gcaatggcgc
    47581 aggtagaagg cgcgggtaag ccgctgagta atttgcaaaa cgggcaaatg gtgaaaatcc
    47641 gccagaacgc cagcggcgtg gtgacgggct taaccattga tacggggaat aatcaacagg
    47701 tcctgtttac ccgccagccg gatggcagtt ttattcgtgc gcggtagatg aagggtcaac
    47761 caaaagccag ctaaggattg tctctttgat gaccttacgt ctggcttcca gtgcctggga
    47821 ggaaaccata ctgatatgtt gacatcctcc acgccctgaa ggacgtggat ccctgcttcg
    47881 ttcaggctgt cgcctgaatc atttcggtgg gttcctgctt caacgagcgg cctgactgca
    47941 ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa aatgttacga gcgccgttta
    48001 catcggcgtt cgctgtatat ccacatacct ggcatctgaa tt
//
LOCUS       DAZGSP010000030        47150 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZGSP010000030 DAZGSP010000000
VERSION     DAZGSP010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.030"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(87..1034)
                     /locus_tag="ACHK74_001842"
     CDS             complement(87..1034)
                     /locus_tag="ACHK74_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP0220673.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINTARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
     gene            complement(1106..1702)
                     /locus_tag="ACHK74_001843"
     CDS             complement(1106..1702)
                     /locus_tag="ACHK74_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP0220674.1"
                     /translation="MNNTDLIHLIKRFMRNEHKAVEEVIDSPLSEVANLIKILQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            complement(1671..2879)
                     /locus_tag="ACHK74_001844"
     CDS             complement(1671..2879)
                     /locus_tag="ACHK74_001844"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFP0220675.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQDFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSALTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNVIATI
                     VAYNECEEWLKSLKVHIASNIQFTLQYLNENIPELIVWPAEGCYFSWVDCSALGYPFD
                     KFYRRLINEGRVGIMAGHVYGTEGESYLRLNLACSREKLYMGLARLASVIKNINQEKE
                     NEQYGSYPSY"
     gene            complement(2879..4459)
                     /locus_tag="ACHK74_001845"
     CDS             complement(2879..4459)
                     /locus_tag="ACHK74_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP0220676.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMGK
                     FIPVLAIPLIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKEKDDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVAADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLFIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRRAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGLANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPLAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEETAIANIPVHDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEAAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGIC"
     gene            complement(4491..5315)
                     /locus_tag="ACHK74_001846"
     CDS             complement(4491..5315)
                     /locus_tag="ACHK74_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP0220677.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVGIEYIFVAEKIVHYAESNLEKKLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLEEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQKITELIEVVYNRRVDRSSFNYSRFMMHLKYFSSRVLCNEK
                     IKQKNIGDIYEQFLEKDFQLQRAIHEIERYLYATFKYDLILEEKLYLSIRIKVLMD"
     gene            complement(5575..6828)
                     /gene="amiC"
                     /locus_tag="ACHK74_001847"
     CDS             complement(5575..6828)
                     /gene="amiC"
                     /locus_tag="ACHK74_001847"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFP0220678.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            7060..8391
                     /gene="argA"
                     /locus_tag="ACHK74_001848"
     CDS             7060..8391
                     /gene="argA"
                     /locus_tag="ACHK74_001848"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFP0220679.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(8453..10279)
                     /gene="recD"
                     /locus_tag="ACHK74_001849"
     CDS             complement(8453..10279)
                     /gene="recD"
                     /locus_tag="ACHK74_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFP0220680.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(10279..13821)
                     /gene="recB"
                     /locus_tag="ACHK74_001850"
     CDS             complement(10279..13821)
                     /gene="recB"
                     /locus_tag="ACHK74_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFP0220681.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIATVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSVGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVELAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(13814..16702)
                     /gene="ptrA"
                     /locus_tag="ACHK74_001851"
     CDS             complement(13814..16702)
                     /gene="ptrA"
                     /locus_tag="ACHK74_001851"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFP0220682.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(16877..20245)
                     /gene="recC"
                     /locus_tag="ACHK74_001852"
     CDS             complement(16877..20245)
                     /gene="recC"
                     /locus_tag="ACHK74_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFP0220683.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLDSSQELDAFVDVMPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLIPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(20258..20581)
                     /gene="ppdC"
                     /locus_tag="ACHK74_001853"
     CDS             complement(20258..20581)
                     /gene="ppdC"
                     /locus_tag="ACHK74_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFP0220684.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(20566..20973)
                     /gene="ygdB"
                     /locus_tag="ACHK74_001854"
     CDS             complement(20566..20973)
                     /gene="ygdB"
                     /locus_tag="ACHK74_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFP0220685.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(20970..21533)
                     /gene="ppdB"
                     /locus_tag="ACHK74_001855"
     CDS             complement(20970..21533)
                     /gene="ppdB"
                     /locus_tag="ACHK74_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP0220686.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(21524..21994)
                     /gene="ppdA"
                     /locus_tag="ACHK74_001856"
     CDS             complement(21524..21994)
                     /gene="ppdA"
                     /locus_tag="ACHK74_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP0220687.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(22179..22973)
                     /gene="thyA"
                     /locus_tag="ACHK74_001857"
     CDS             complement(22179..22973)
                     /gene="thyA"
                     /locus_tag="ACHK74_001857"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP0220688.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(22980..23855)
                     /gene="lgt"
                     /locus_tag="ACHK74_001858"
     CDS             complement(22980..23855)
                     /gene="lgt"
                     /locus_tag="ACHK74_001858"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP0220689.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(24006..26252)
                     /gene="ptsP"
                     /locus_tag="ACHK74_001859"
     CDS             complement(24006..26252)
                     /gene="ptsP"
                     /locus_tag="ACHK74_001859"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP0220690.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYADAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(26265..26795)
                     /gene="rppH"
                     /locus_tag="ACHK74_001860"
     CDS             complement(26265..26795)
                     /gene="rppH"
                     /locus_tag="ACHK74_001860"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFP0220691.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(27148..27294)
                     /gene="ygdT"
                     /locus_tag="ACHK74_001861"
     CDS             complement(27148..27294)
                     /gene="ygdT"
                     /locus_tag="ACHK74_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFP0220692.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            27480..28169
                     /gene="mutH"
                     /locus_tag="ACHK74_001862"
     CDS             27480..28169
                     /gene="mutH"
                     /locus_tag="ACHK74_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFP0220693.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            28238..28951
                     /gene="ygdQ"
                     /locus_tag="ACHK74_001863"
     CDS             28238..28951
                     /gene="ygdQ"
                     /locus_tag="ACHK74_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP0220694.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            29089..29307
                     /gene="ygdR"
                     /locus_tag="ACHK74_001864"
     CDS             29089..29307
                     /gene="ygdR"
                     /locus_tag="ACHK74_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFP0220695.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSNDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            29415..30455
                     /gene="tas"
                     /locus_tag="ACHK74_001865"
     CDS             29415..30455
                     /gene="tas"
                     /locus_tag="ACHK74_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFP0220696.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAT
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(30487..31680)
                     /gene="lplT"
                     /locus_tag="ACHK74_001866"
     CDS             complement(30487..31680)
                     /gene="lplT"
                     /locus_tag="ACHK74_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFP0220697.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(31673..33832)
                     /gene="aas"
                     /locus_tag="ACHK74_001867"
     CDS             complement(31673..33832)
                     /gene="aas"
                     /locus_tag="ACHK74_001867"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFP0220698.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            34418..35449
                     /gene="galR"
                     /locus_tag="ACHK74_001868"
     CDS             34418..35449
                     /gene="galR"
                     /locus_tag="ACHK74_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFP0220699.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(35456..36718)
                     /gene="lysA"
                     /locus_tag="ACHK74_001869"
     CDS             complement(35456..36718)
                     /gene="lysA"
                     /locus_tag="ACHK74_001869"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFP0220700.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHVPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            36841..37776
                     /gene="lysR"
                     /locus_tag="ACHK74_001870"
     CDS             36841..37776
                     /gene="lysR"
                     /locus_tag="ACHK74_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFP0220701.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(37763..38455)
                     /gene="ygeA"
                     /locus_tag="ACHK74_001871"
     CDS             complement(37763..38455)
                     /gene="ygeA"
                     /locus_tag="ACHK74_001871"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFP0220702.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(38584..40002)
                     /gene="araE"
                     /locus_tag="ACHK74_001872"
     CDS             complement(38584..40002)
                     /gene="araE"
                     /locus_tag="ACHK74_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFP0220703.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(40317..41078)
                     /gene="kduD"
                     /locus_tag="ACHK74_001873"
     CDS             complement(40317..41078)
                     /gene="kduD"
                     /locus_tag="ACHK74_001873"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFP0220704.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGISGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(41108..41944)
                     /gene="kduI"
                     /locus_tag="ACHK74_001874"
     CDS             complement(41108..41944)
                     /gene="kduI"
                     /locus_tag="ACHK74_001874"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFP0220705.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(42231..43412)
                     /gene="yqeF"
                     /locus_tag="ACHK74_001875"
     CDS             complement(42231..43412)
                     /gene="yqeF"
                     /locus_tag="ACHK74_001875"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFP0220706.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PHTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            43667..44896
                     /gene="yqeG"
                     /locus_tag="ACHK74_001876"
     CDS             43667..44896
                     /gene="yqeG"
                     /locus_tag="ACHK74_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0220707.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIDNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            45356..45988
                     /gene="yqeH"
                     /locus_tag="ACHK74_001877"
     CDS             45356..45988
                     /gene="yqeH"
                     /locus_tag="ACHK74_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0220708.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            46322..47131
                     /gene="yqeI"
                     /locus_tag="ACHK74_001878"
     CDS             46322..47131
                     /gene="yqeI"
                     /locus_tag="ACHK74_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0220709.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVNRD"
BASE COUNT        12201 a        12364 c        10989 g        11596 t
ORIGIN      
        1 cgcaaccaat acttagaatg ctgataaacc gcaatgtgtt ccacaaattg cggttttttt
       61 gtatttaaaa acttcgttcg ctgaactcac aacacgtttt ttatctccct tcctgcgaag
      121 taatcaatta tcgactgggc aacgtgtgta ccggtttgct ggtatgactc aacggtggcg
      181 gccgcaatat gcggcgttgt aatgatattt ttggcatgta gcagtgggct aaattgcgcc
      241 aacggttcag agctaaacac atccagcgcc gcaaacacat ttcgttcttc aatcacctgt
      301 agtaaagcgc cctcatccag caactttccc cttgcggtat tgatgattag agcaccattg
      361 cgcatcatcg acaaatattt atgactaatg aagttctcca gctcaggaga aaaaggcaaa
      421 agaacaatga tgacatgcga ctgcataaag atatcttcaa ttgatacaaa ctgaactcca
      481 actttgtcag caacctgata atcgggttgt ttgtcataag ctaaaagctc aacatcaaaa
      541 ccggataacc tttttgccag ggttttacct atattgccgt agccaaccat gccaatccgt
      601 ttgccttgta attcatagcc atgcgactca ccccagtagc cattttgcat cgcatgatag
      661 ctgcctggaa tatttctcat gctcgcgaag ataagtccaa tgataaattc cgcgacagca
      721 tttgaattaa tcccaaccgc attattgact acaataccgg attgttgtgc tgcacgaaga
      781 tcaatattat ctatccccgt cccgaatctc gatattattt tcacaccagg cagcaatgat
      841 agcgtgtccg cctgataaca atcttttccg gcgatgaccg cgcaaataga ttctcgtaat
      901 gcagtggatt ctgatttaag cagatgctcc acattattct taataataag attaaaatta
      961 ttctctctta gcaacttgct ggcggaaaca cagcgtgctg aaaagctggg gacggtgacc
     1021 agaacatttt tcatagctga cactcacttc aatgaacgcc accccaaaag atggcgtgac
     1081 cggttactgt atttatactt tgaaattatg aatttgcttt aatgccgagt gcacctcctg
     1141 gatgatataa gccgaaatca gcacgagtaa attttttcaa ttcagagagt gcaagcgcca
     1201 ccgcatcacc aacaaccaga acaacagtag acgagcatga cggtgctaaa cctaaattat
     1261 ccgcctcact tttgacgggg atttcaacag ataaatcaca acttatcgca agcgatgagt
     1321 gatgactgcg ggtaaaagag atcaaataat tacccatttt tttcaaacta ggcagtgtcg
     1381 ccaggatttc cgccgtctca ccactgtttg aaataagaat aacaacatcg tctttcgcca
     1441 ccatcccaag gtcgccgtgt accgcttccg taccgtgaac aaaaaacgag ggagttccgg
     1501 tgctggcaaa tgttgcagca agttttctgg caataatgcc agatttccca acaccaataa
     1561 aaacaacttt tccctggcaa gattgtaata ttttaattaa attcgcgact tccgaaaggg
     1621 gggaatcaat aacctcttct actgctttat gttcgttacg cataaaacgt ttaataagat
     1681 ggataagatc cgtattgttc attttctttt tcctgattaa tatttttaat tacactcgca
     1741 aggcgcgcaa gccccatata taatttttct cgactgcagg ccagattaag acgcagatag
     1801 ctctctcctt cagtaccata aacatgcccc gccataatac ctactctccc ttcgttaatt
     1861 aatcgacggt aaaatttatc aaagggataa ccaagggccg agcaatcaac ccacgaaaaa
     1921 tagcagccct cagctggcca tacgatcaat tccggaatat tttcatttaa atactgcaag
     1981 gtaaattgaa tgttagaggc aatatgtacc tttaacgact ttaaccattc ttcacattca
     2041 ttataggcaa caatggtcgc aattacattc agtactgatg gggagtttac gaaatcgcga
     2101 tacctcgata tcgttctgaa cttatgatta aaattatcgt cagttgaaat catatatgat
     2161 ccggttagcg caggaacatt aaatgttttc gaaatcgaat taaacaccac cactttgtca
     2221 tcttttattg ttagcgctga cgtgaaacta tgattaaaaa caaaatcact atgtatttca
     2281 tctgaacata accaaatatt atgccttgca cagcatgctg cgattctgtg aagatcgtct
     2341 tccgaccaga ccgttcctgt cgggttatga ggattacata ataacaccat cttcgcgctt
     2401 ttaattttaa gctcaaagtc ttgccagttg ataacgtagc ctctcccatt ttctttcatc
     2461 aaagaggttg ttagcatgtt tcgaccatta tgcttaatca catcaaaaaa tgcgttataa
     2521 acaggcgtga aaatgagaat attatctcca ttcaatgtca atatttctat tgccttagat
     2581 attgaataca tcacgcttgg gctataggtg atccattctg gttgaatctc tgttttatat
     2641 tgttgatgat accaattgac tatcgaattc agatagtcat catgctgcca acgactgtac
     2701 cctaaaatcg ggtgttccag acgttttttt aatgttctga tgatagcttc cggtatcgga
     2761 agatccatat ctgaaataga gaatggtaat acatctttta caccaaatct gtcttcacaa
     2821 aagtcccatt gtgtgcaata tgttccttta cgagaaattg aggtattaaa aatatccatc
     2881 aacatatccc catgtaatta tccatatccc gacgtagggc gatgactttt gtaccgataa
     2941 tgacttgtaa ggtattttga tcaagtttca caaccgcaat tcctccggcc ctttttattg
     3001 cagcctcatc tatcaatccc atatccgcga tagttaaacg caaacgagtt acacaattat
     3061 ccagtgattt aatattttct ttaccaccca gagccttaac catttgctcc gctgcctgca
     3121 actctgattt atcatgaact gggatgttag ctattgcagt ttcttcgtga gcgttcagcg
     3181 tgacaggact ggactcttca cggcctacag tcatcaggtt aaatttgaga atggtccaac
     3241 ggaacacaaa gtaataaatg aagaaataag ccacttcaac acataccgct aatggccatc
     3301 ccgttcttgt gccacgtaag acaccaaagc taataaattg aatcagatcg ccagtaaagc
     3361 ccatcttcac gcccatatat ggcagaacca tgttagccaa tccattcata aaggcatgga
     3421 agacaaacaa cagcgggctg acaaataaga acagaaattc aatcggctcg ctgacgccgc
     3481 caataattac cgtgagcaca ccggaaatca gcaatgcttt gatagccctt ctgttttgag
     3541 catgagcagt gcgatacatc gccagcgcgg ctccagccag accatactga atcatcagtt
     3601 tgccctgctc cattttcccc gccaaatcca gcggaatgac cgcattctct ttgacgtatg
     3661 ccataaacat gttcagggtg ccgtaatact cttcaccgcc aatcactgcc gatccgccga
     3721 ttggggtaaa gcggaaaact gatgtcacca agtgatttaa gccaaaaggt atcgtcacgc
     3781 gttcggcaac agcatagaag aaataaccaa caggaccgga agttgaaatc cagtgaccaa
     3841 tgagcatgaa aagattgaaa aatggcggcc agataaaagg aatgaacaga ccaaatccac
     3901 tcatcacaat cagtgtaatg ataggcacaa ggcgtgggcc actataaaac cctaatgatt
     3961 caggaatacg taagttaacg atctttttat acatgctggc gactaataac cctgccacaa
     4021 tcccacccaa cacgctggta ttgtaggact ggatcccgag tatgatggtt tgcccatgtg
     4081 tcgacatttg gtcagcagct accaataagt cgtgctgttt aagataaaag ttcgttccaa
     4141 gatgcatcgc cataaagcca attaagccag aaaaagcgcc ataggcttta tcatctttct
     4201 cttttaataa tcccaaaggt atagctatcg caaacaatac aggtaaatta acgaaggcaa
     4261 acaaaccaag gctaacaata aaatcaagta tggttttaat taacggaata gccaacactg
     4321 gaatgaattt tcccatatca tcgctggcta aaccacttcc cagccctaac atcataccac
     4381 atacacttaa cagagcgatg ggatacataa atgccttccc caggctctga aaaaaactcc
     4441 aggctttctt ttgtttcata ctggttatct catgaaaatg ttatgtataa ttagtccatt
     4501 aatactttga tgcgaataga aagatatagt ttctcttcca gaatcaaatc atatttaaaa
     4561 gttgcgtaca gatatctttc aatttcatga atggcacgtt gtaactgaaa atctttttca
     4621 agaaactgct catagatatc accaatattt ttctgtttta ttttttcatt acataacaca
     4681 cgactagaaa aatatttaag gtgcatcata aatctggagt agttaaatga acttctgtcc
     4741 acccttctgt tataaacaac ttcaataagc tccgtgattt tttgagacag ctcaataatc
     4801 cttactgatt cataatcaac tgaattttca taattattta aaatatgcaa tgctataaaa
     4861 ccaatctcat catccggtag ttgaacgcta aattgctcat taatgattgt taatgcatca
     4921 cgacttattg catactctgc tttgtacaac gctttgattt cctcaaggaa aacattatta
     4981 atttgtatgc ctgagacgac tcgggatatt gcattcgaaa tgtgatcagc aagaatcagt
     5041 agtagcgatg gattgagttt tttttcgaga ttactctccg cataatgcac tatcttttcg
     5101 gcaacaaaaa tatactcaat acccacatgc tcaatcaatt tgtagagttt atttttttgc
     5161 tcatttctga cataaaaaat ccggtttgca ggatgctcag ggaccgacat accatatttt
     5221 ttattgtaac caacgccagg cccagaaatg ataacctcct gtccgttcac cgatgcctgt
     5281 acacaattat tgttcatgac tttttcgatg atcattccat ggctcccaaa aaaaaaggca
     5341 aggcatgtca gaagtaataa ttacttcaaa cattgccttg cctgatttaa cagttacaag
     5401 ataattaact aatgcgtata ccttacacgg aattgtgctt aataaaagag atgacaggct
     5461 tattttttaa gccgatattc aaatattcga cagcagtcac aaaaccatcg ttcaacattt
     5521 atgtaaataa tatttattca atgaaacata aattaattga ataaattaaa aacatcatcc
     5581 ccttctcgcc agcgtcgccc catcggcaaa atacgcttta atccccgcaa gaatagactc
     5641 cgcaacttcc tgctgaaagg tcgccgtttt cagtttacgc tcttcctcaa cgttactgat
     5701 aaacgccgtt tcgaccagaa tggagggaat atctggtgcc tttagtacgg caaacccggc
     5761 ctgttcaacc tgatttttat gcagcttgtt gattttaccg agcttattca gtaccgcttt
     5821 accaaactta aggctgtcag caatggtcag cgactgcacc atatcgaaca tggtgtggtc
     5881 gacatagcgg tcaccgcttt tgctcacgcc accaatcaag tccgaggcgt tctgggtttg
     5941 tgccagatat tttgccgcag tactggttgc acctttggtt gagagcgcaa acacagagga
     6001 accgctcggc tgacgactgg taaaggcgtc ggcatggata gagacaaaca ggtcagcacg
     6061 ctgtttctgg gcttttgcta cgcgcacttg caacggaatg aagatgtctt cattgcgcgt
     6121 catgtacacc ttcatattgc cctctttctc gatcagagag cgcaaacggc gagctatttg
     6181 caataccaca tctttttctc gcgttttgta tttccccacc gcaccggagt cttcgccacc
     6241 gtggccaggg tcaagcataa tgacaatcgg acgatcgcgc cctgctttac ccggttgtgg
     6301 accactttgt gctggcggca cctgcttttc gaggtcgcct ttgttgtaat cctccagcag
     6361 cgccagcagc gggtcctgca tatcctgtgc attggccgga tagaggtcca tcaccagacg
     6421 ctctttaaac ccggcgaccg gcgcaagggc aaacagctgc ggttttacgt tttgctttaa
     6481 ttcaaaaacc atccgtacgg tctgcgggtc aaattgcccg acgcgcgccg acttgatgaa
     6541 cgggtcgtct gcacgaattt gtgccgccat ccccttgagt accgagttca ggtttacatc
     6601 ttcgatatcc accaccacgc gttccgggtt actcaacgcg aactgcttat atttcagctg
     6661 acgatttgat tctaccgtca cacgggtgta gctggacgca ggccagacgc gcaccgccac
     6721 gacctggctg accgcagcca gactgacctg acttacgctc aatagccaca tggcacccgc
     6781 gccttgcagt aaacgacgac ggctgattgc agtgttggat cctgacatgc ctctcccgag
     6841 caaaagaaat ctaagctgtg taacaagtaa acgactaatt tgaccggaaa ctttagcgaa
     6901 agacgcataa tctgtcatct aataaacggt aaacattctt tttatattca cggcattact
     6961 gataaaaaag tcgctctcgc ataaaattta cacttgcacc ctgcgaaaaa acagaataaa
     7021 aatacactaa tttcgaataa tcatgcaaag gggtgtgccg tggtaaagga acgtaaaacc
     7081 gagctggtcg agggattccg ccattcggtt ccctatatca atacccaccg gggaaaaacg
     7141 tttgtcatca tgctcggcgg tgaagccatt gagcatgaga atttctccag tatcgttaat
     7201 gatatcgggt tgttgcacag cctcggcatc cgtctggtgg tggtctatgg cgcacgtccg
     7261 cagatcgacg caaatctggc cgcgcatcac cacgaaccgc tgtatcacaa gaatatacgt
     7321 gtgaccgacg ccaaaacact ggaactggtg aagcaggctg cgggaacatt gcaactggac
     7381 atcactgctc gcctgtcgat gagtctcaat aacacgccgc tacagggcgc gcatatcaac
     7441 gtcgtcagtg gcaattttat tattgcccag ccgctgggcg tcgatgacgg cgtggattac
     7501 tgccatagcg ggcgtatccg gcggattgat gaagacgcga tccatcgtca actggacagc
     7561 ggtgcaatag tgctaatggg gccggtcgct gtttcagtca ctggcgagag ctttaatctg
     7621 acctcggaag agattgccac tcaactagcc atcaaactga aagctgaaaa gatgattggt
     7681 ttttgctctt cccagggcgt tactaatgac gacggtgata ttgtctccga acttttccct
     7741 aacgaagcgc aagcacgggt agaagcccag gaagagaaag gcgattacaa ctccggtacg
     7801 gtgcgctttt tgcgtggcgc agtgaaagcc tgccgcagcg gcgtgcgtcg ctgtcattta
     7861 atcagttatc aggaagatgg cgcgctgttg caagagttgt tctcacgcga cggtatcggt
     7921 acgcagattg tgatggaaag cgctgagcag attcgtcgcg caacaatcaa cgatattggc
     7981 ggtattctgg agttgattcg cccgctggag cagcaaggta ttctggtgcg ccgttctcgc
     8041 gagcagctgg agatggaaat cgataaattc accattattc agcgcgataa cacgactatt
     8101 gcctgcgccg cgctctatcc gttcccggaa gagaagattg gggaaatggc ctgtgtggca
     8161 gttcacccgg attaccgcag ctcatcacgg ggcgaggttc tgctggaacg cattgccgct
     8221 caggcgaagc agagcggctt aagcaaattg tttgtgctga ccacgcgcag tattcactgg
     8281 ttccaggaac gtggatttac cccggtggat attgatttac tgcccgagag caaaaagcag
     8341 ttgtacaact accagcgtaa atccaaagtg ttgatggcgg atttagggta agcgatgaaa
     8401 attgtcggat gcgacatgcg taacactcgt gcgtcgcatc cggcaattac gtttattccc
     8461 gcgaactaaa caacgccgcc agaccactgc gccgctcagt acgagtggcg attgccgcac
     8521 ttaatatacg ctcatcggca tacagcgaca gacggcgacg cgcgcgggtc accgcggtgt
     8581 ataccagctc tcgcgttact accggcgtgc gttggctcgg taaaatcaac gccgcatggt
     8641 cgaactccga tccctgcgat ttatgtaccg tcatcgccca cgtagtttcg tgctctggca
     8701 agcgactcgg ttgcacagac ttaatattgc cgtccggcat cgcaaaccag acgcgcgtcc
     8761 cctgcccgcg atccagcgca ataccgatat cgccattaaa caacccaagc gcgctgtcat
     8821 tacgggcaat catcaccggt cgaccttcgt accaacgaga gtgcggatga cgatgaattt
     8881 tgcgcttctg ttgcataaac tgctcaattc gctcattcag tccagccacg ccaaacggcc
     8941 cttcccgcag ggcgcacaaa agttggtact cattgaacgc ctgaatgatt aaatccggct
     9001 cggcacgcgc ttgcagcaga tccagataac gtccataacc cgcaagagct tcctcaagca
     9061 tcgcaatata atcttcgccg ctctgtaaaa gtcgtttttc gatatcagta aaatcctgct
     9121 gaaaaacggt tttcactgcc gttttatcac cacggttgat cgccgcagct aactgaccaa
     9181 tgccagaatc gctgccgaaa cgatagcttt tttgcagcag gcagagactg tcgcgcaaag
     9241 atgccgcttc tgtgccggtt cctgccggaa cgtgagtccc cgtcaggcgg cttagctgcc
     9301 cggcacgctc ggcggtaaag ccggcattgg cataagcgca gatatcgccc agcacagccc
     9361 cagcctcaac cgaggccagt tgatcacgat cgccgagaaa gatcactcgc gcatgatcgg
     9421 gcaaggcgtc gatcagtctc gacatcatag gcagatcgat cattgacgct tcatctacca
     9481 ccagcacatc aagatgcagc gggttaccgg catgatgacg taaacgctgg ctacccggct
     9541 gcgcacccag caatcggtgc aaagtactgg catcttccgg aatgcgtttc ttttgttcat
     9601 cggtcagcgg taactgtcgc aaagccttgc cgagagattc ggttaagcgc gcggcagctt
     9661 tacccgttgg tgcagccaga cggatacggc agcgttcgcc gtcggccatt tgaattaacg
     9721 ctgccagtaa cttcgccacg gtggtcgttt taccggtgcc tgggccgccg gaaatcaccg
     9781 agatccgccg cgtcagcgcc actgccgctg caactttttg ccagttaatt tcatcgctta
     9841 ctggaaaaag tttgtccagg gtttgcgcca gtagagcttc atcaacctca atggcatgat
     9901 tcacctcatt gaagaagcgc gctaccgtcc gctcgttaca ccacatgcga ttcaaataaa
     9961 gacgatcgcc acaaaggatc atcggcgtgg gttcatctcc cctgctgacc gcttgagaag
    10021 ccagcaagca ttcttcccaa ttttgtagct caccgatttc actgacacag gtcgccaaca
    10081 gcggatgcga cgcctcgtta ttttccagtc gtgaaagcgg caaacagacg tgtccctctc
    10141 cggcatcatg acttaacagt gccgccgcga gggtgacggc aggatgttca tctcccgcca
    10201 cggtcagggc aaattgcaca tccagcgggc gtagctgttt gtgctccaca gcttccagta
    10261 attgcttttg caatttcatt acgcctcctc cagggtcata ccggcaaaca tctcatccat
    10321 cagggcaatc aacccggcgt tgggtcgggt tgtgtaaatc ccttgttgcg gatgttcttt
    10381 atcaacgcca cgcaggaaca gatagataac gccgccaaag tgacgatcat agtcgtaatc
    10441 agcaatgcga tggcgcagat aacgatgcag cgccagggta taaagctgat attgcagatc
    10501 atagcggtgt gcctgcatcg ctgctgccat agcctgttgg gtgtaagccg aactgtcttc
    10561 acccagccag ttggatttat agtcgagcag gtaataacgc ccttcgtggc ggaatactag
    10621 gtcgataaag ccttttaaca tgccgcgtac ctgcatgaac tccagcggcg ggcagccagc
    10681 ggatagcggg tcaaactggc gaattagtgc atcaagctgg ctggcgatga gcggttcgct
    10741 aatcggcagg taaaactcca tctccacctg tttatcgcgg tcggaaagct gactcaggct
    10801 aacacccgtt tcattgagag gtgcctggag gacagccgtg atccactcgg tcaacaccgg
    10861 ttcccactgc gattcaaagc cgccgagttc cagtttttcc tgtacccagt tcgggtcaac
    10921 cggctgggta aaatcgaggt cttcaaacaa actgtgcaag aatgtccccg gtgacgcacc
    10981 gcgcgggaac tgatgcggtg ttaacgtcgg ttcttcaacg acgctgacca cgcctgcggc
    11041 atcaacatcc agccgtggca tcagatcctg agcgataccg tgaccacgct gctgcaagcc
    11101 ggagtagctg gtgacacgcc agttatcgcc gggcaatcgt tgtagcgtcc tcgcattcag
    11161 ttctgcagtt aaagcatcat taacctgcca gggctggtta tcaccagttt gtgccgtttg
    11221 ccaggcaata tcatcatcgc ataacgcttc aatacaggtg cgaagccctg ccgcatcttg
    11281 cggttcccct ttttgcagca aacgcccgag cgcactttgg tggacgtcgg tgtcaccttt
    11341 tttatcgcca cgacggcgca ccagcggtgc aacgccgaga ctgcaatgcc aaaccgaacg
    11401 cgtcagcgcc acgtaaagca aacgcagatc ttccgccaga cgttcgacct ccgcaagctc
    11461 gacgctttct ggcgcagcgt taagatccag aacagcctca aacgagtggc gatcgtgata
    11521 aaacgcctga tcctggacgc ggaaattggt aataaatggc aaccagacca gcggatattc
    11581 cagccctttt gatttatgga tcgtgacaat ctgcaccaga tgtttatcac tttcgaggcg
    11641 catttgttgg ctggaggcat tactgtctgg ctcgaggata tgttgcgata accagcgtac
    11701 cagcgcatgt tcactttcca gctgcgttcc ggcttcttgt agcagttcgc tgatatgcaa
    11761 gatatcggta agtcgccgct caccgcctgc cgttgccagc aagttttctg caatgttacg
    11821 cgccgacatc agcgcccgca gcatcggcat aacgccacgt ttgcgccaga tttgccgata
    11881 accatcgaac tcttcgacca ccgcgtccca cgcatgttcg tcattattca gcgtttcaat
    11941 atccagcgca ttcagcccca tcattgacgt tgccagcgca ctgcgcaggg tgttctcacg
    12001 ttcgggcgtc atcaccgcct gcaacaacca aagcatttcc tgcgcttcca gagtttcaaa
    12061 aacactgtcg cggttcgaaa ggtaaacgga agggatttcc agcaacgtta aggcatcgcg
    12121 cacctgggcg gcctcctggc ggctgcgcac cagcacactg atgtccgaag cacgcaccgg
    12181 acgcgcgtcg tcgccgttca tcagcaacgc ttcgccccgc tgtccggctt gtagccagtc
    12241 gcggatttgc gcagcacata cctgcgccat ggtactttga taatcgccaa cgccgcagct
    12301 ttcgccttcc atcagccaca ttttcatcgc aggctgcgtt tcacctttaa atacaaaacg
    12361 taacgcctga tttttcccga ctgatttcac tggaataaac ggtatttcgc gaaacatgaa
    12421 cgcgtcatca gtctggctga aaagcttatt cacgctgttc accattcctg gtgcggaacg
    12481 ccagttggtg tctaaagtgt agtgggcgtg aacttcgcta cgcgccttca tataagtgaa
    12541 gatatccgca ccccggaatg catatatggc ctgcttcggg tcgccaatca gcaacaatgc
    12601 ggtttccggc tgatggtgcc agatacggcg aaaaattcgg tactgctggg ggtcggtatc
    12661 ctgaaattca tcgatcattg ccaccgggaa tcgcgtacgg atcgccgctg ccaacacctc
    12721 accgctttcg ctacgcagcg cggaatcgag ccgacttaac atgtcatcaa aacccaattc
    12781 gccacggcgg cgtttttcac gcgctactgt ttcgcggatc tcagccaatg cgcgggtgat
    12841 caccagatcg cggatcgaca atggttctgc aagcagttga tcgatcgcct caaacagtgg
    12901 atgtcgcggg gtttcccccc cggctttcgt gcgatcttct aagaaacgct gggagaattt
    12961 ttccagcgac tccggcaact gataactgtt ggtctcttct tctgcccagg cgctgatctt
    13021 gtcgatccat ttagcctgat tgctacggtt aaacttgcgt cgatcaatac cagaagattc
    13081 gatcagcgca tccagttcac ccactgcgtc gcgccactgc tgttttaccg tagcaatacg
    13141 cgccacaatt tgcgcgtggc gggaagccag cgtttcatca tcgggcggcg gtgctttgat
    13201 aaccggcgct tcgccttgca gataacgatt aatatcgcgc agcaacgcct gcggcccttt
    13261 ccaggtttca aagacgacct gggctatttc acgcggcagc gggtagcagt ggcgacgcca
    13321 gaaatcggcg caggcctggt aacgtagcag agactcatct tcaatcagct gctgctcaaa
    13381 cagcatgccg gattcaaagg cattcaggtt gagcatgcgc tggcaaaaac cgtgaatagt
    13441 aaagactgcc gcttcatcca tctgccgttc ggctaacaac aaccactgcg cggcttgcgc
    13501 tttatcgtcg atctcttcca gcagacgttc gtacagtgga ttgtcggtgg tttcacgcag
    13561 acaggcgatg cgcaactcat ggatattgct acggatacga ccacgcaatt ctgccgtggc
    13621 agcctcggta aaggtgacca ccagcagttc ttcaacggtc agcgggcggg gaaaggcggc
    13681 ggaaccgcct agtccaagta acaggcgcaa atagagcgcc gcaatcgtaa aggttttgcc
    13741 tgtgccggca gaggcttcaa tcaggcgctc accctgtaag ggcaagcgca aaggatctag
    13801 tgtctcggcg acatcactca ttcttttcac tcatcagggg cattgtttgc tgcaacgcgc
    13861 tgacgttctc ccacactttc cagccttcag ggtgtacata ttcggctttc ccattctggc
    13921 tgccggaaat ctgcgacagg atagccatgc cttgcggctc gactaccgcc tgatggaaga
    13981 aatcggccag tttttgcggc gtcagcagtt taatctgggc cacgatttta tcacgcgaat
    14041 cgaagcgcat attgccgcga tcgaaatctt tacttaactt cgatgcttct tcgccgagcg
    14101 tttgcggtgc ctgcagcatc tgggtaatta ccgcctgctg gatttgcgca aactcttctg
    14161 gcttcatcgc ccgcaatttt gcctctgcgg ttgggaaaaa cgccttgtaa cgctcccaca
    14221 agaatgaagg ctgtttatca ttgctttgca aaaggaagcc catgccccac tgacgcccca
    14281 cgctcattgg gaatgcaaac acggcatagc ccaactgttc ttcggtacgc aactgattgt
    14341 agaaccacgg ctgtacgatc tgccccaaca gtgagctata ggctgagctg gtgtattcat
    14401 cgtagccagt cggtacaaat accgctgcca gtgcggagtc ggtgctgtta ccggcttttt
    14461 caaagatgac ggattgtttt ttatcgacca cgacatcttt gttacgacac cactctgaac
    14521 catcagcgcc caactgtttt tgcacatcgc gtgccagcgt tgttgcctgg gcttcggtca
    14581 tgttgccaat aaccataaac tctggtcgag cccctgattt taaggcgtcg cgataggcca
    14641 gcacctcttt caacgtaatg gagggcaaaa ttttgcgccg ttcatctcgc gagaagtacg
    14701 gcacttgcga gagcatctgc gcgggcataa tcgcctgctc gaacgctttg cccttttctg
    14761 cggaatccat catctggtta taccaggact ttgcctgctc aagctgatct tccgtggcgg
    14821 tatagctaaa gtagccctcc agcaatgcct ggaacagctg cggcagacgc tgggtgtaac
    14881 cattagcatt aaccataagg ccgttgttag cgttggtgga aaaacttatg ccaccaaccg
    14941 acgcctggtt gcttaactga tcaagcgcca gccctgcgag ataatcattg agcgcaaaca
    15001 tcacctgatt gcgggcgctg tccatggctt tcggattacg caaaatcagg ctgacatcag
    15061 ctttgggttc gctggaaaaa taacggcttg gcgcatacac cacacgcaga ttcgactcat
    15121 caacaatcag ctctggatgg tcatatttct tctctgactt aatcagcgag aaatcgtcag
    15181 gaatataggg gttaagctct ggcaaagaga gcgcaatgtc ggcggctttt ttctgccagt
    15241 cggcgaaagt ttgtgcgctg attttatcga cctgatacgg cgcatcgaca aaataagccg
    15301 ttttattgtg cggctctttc gggctgatat accagatacg cgcattctgc ggcgtcatca
    15361 tcgccagacg ttcttttact gctttagcat cgtaccgatc ggcaatattg actgcatcca
    15421 gcgtatgctc aacaggaacg cgaatcatgg tatctgccag ccattcgacg taatccatat
    15481 cacgggtgat tgacggataa cggaagtcga tatccagcac attcgccagt tcatcgaagt
    15541 attgtttatc aatccctttt tcacgtaaca aattgaggta gctaaaaatg gccgccacaa
    15601 cctgatcgcg attcgccaga cctttatcgg ttaaagacgc agagatcgct aatacgccgc
    15661 tgttgccgtt gacgatagga tcggagttgg cgctaatgcc ctcaactaat ccctgctttt
    15721 gcagccagtc ggaaagtgta cctgggctgc gattaccaat cagataggta atcaattcat
    15781 ccgttttact acggaacttc gctgagttgt tatcgatgcg aaactcaacg cgcagaactt
    15841 tacgcggcag tgccgggacg taatgaatga taatgccctt ttgcgcgtcg gtgactaccg
    15901 gcacggtgat ttccggtttt ttgctctctt tgttcggcac gcgaccaaag gtgtccgccg
    15961 ccatttttgc caactccggc aacggtttat tactgtaaat aaccgccttc atcaaattgg
    16021 cggaatagta cttctcgtgg aaatctttca gcgcctgctg caccggatta ccaggtttgt
    16081 cgcttaaagt ttcgaggtta ccgcctgaaa actttgaacc agggtgcgcc gggttaatgg
    16141 tttctgcgct gacctgtgcc atgcgcatcc cgtcacgcgt acgcgccatg gttaattcag
    16201 cgttcactgc attacgctca cgttcggcgt atttcttgtc gagcaagggt tctgcaatag
    16261 catcggccag gcggtctacc gcaccgggca aggcgtcgtt ctcaacttcc agatagaaag
    16321 ccgtgcgata cggcgcagtg ctggcattgt gactaccgcc gtgcattttg agatattcgg
    16381 ccagactgtc agcctgcggg tactttttcg accccatcag actcatatgt tcaaggtaat
    16441 gtgccagccc ctggtacgcc tcgggatctt ccagcgaccc aacgggcacc accagcgccg
    16501 agagcgattt aactgcctgc ggatcagaaa ccagcaagac caccataccg ttatccagac
    16561 gtatagcctg atactggcgg ttatctttat cacttttacg gatggtttcc tgaatcggct
    16621 gccatcccgt ttctgcctga cttaagggtg cccaaagggc aactaacaat aataatgctt
    16681 tgaaccaggt gctgcggggc attcacggac ctcataatca acttaatttt ctgtcagatt
    16741 caacacgtta acgcaaatca tctgccagaa tttaatcttg tgctgcacga gtcagcctat
    16801 gtttatataa ccatcagtcc gtgactggtg cgcatcataa agtaagcgga tagattgcgc
    16861 aatttttata cagcactcat gactgattaa agcgaaacag cggtaacagg aaacgttgcg
    16921 actgttcaac gatggcctcc attgtctctg gtattaattg ccgccagagc ctttgatacc
    16981 agatatcatc accttcgcca cgcaccatca tgttgccttc gtaagcctga aggaatttcg
    17041 tacgggcttt ttgcaacgtg gaatcgtcat ccagcatggc atcgttttgc gcgtcataac
    17101 aggtttttag ccacgcgccg ccactttcag gtaacaccag caatggcgcg gacatccctt
    17161 cacgataccc ctcaatcagt tgtgagaggt aatgcaaagc ctgttcggct gcaagcggcg
    17221 gaaaacgcca ctcgccgtct ttgcgtagaa aaaggcgact ttcaccatta ccaccgctgg
    17281 cacagtagac aaggtgttcc agccaaagtt gcattccctg cgccacactt aataaagagg
    17341 gacgccagcg caacaagcca tccggctgca cctgcggcaa ccagccagtt atctgcacac
    17401 cgttgcaggc gagatcaatt tccatactct gccccggctg gcgacaggca atgactctgt
    17461 cggcaagctg ctgcatctcc tggcactgtg tttcccagaa aatttcacca aaagcgccat
    17521 acggtaaatc ccctgccgcc cggaagcggc ggaacaagcg ttcggcatca tcctgctcaa
    17581 ccagtgcatt caataactgc tgattgattt gataacgact aagcccttca agaataaatg
    17641 gctcggtgtc ggggatttcg ctgtcttcag tacggaagtt cacctgcaaa cgcatctgga
    17701 agaacgcccg caccggatgt gcccagaatc gttgtagcgt ttccagcggc acggtttccg
    17761 gtaaggtaaa cggcagcggc tgaacaaatt cagaatgtgc tttaccagcc tggctggccg
    17821 caggtagcca ttcacgagca tagctttgtc gttcgcctgg ctggtagttt tgtggatcaa
    17881 acggcatccg ggtatggagg caagtaagat gcgcttttac ccttgcctcg ctttcatcac
    17941 agttgagcgc ttcatcgccc ggtagataat gactttgccc gatgtagtcg atcagttcct
    18001 gcaccagtac cgacgggaaa cgttcactgt tatcctgaat ggagcgcccg atatagctga
    18061 tataaagttt ttgttgcgcg gaaattaacg cttccaggaa cagatagcgg tcatcatcgc
    18121 gacggctacg gtcgccacgc ttcggtttct ggctcatcag gtcaaagccc aatggcgcaa
    18181 gctgacgtgg ataaacgccg tcgttcattc ccagcaggca aaccactttg aacggaattg
    18241 aacgcattgg catcagagta caaatgttaa ccggtccggc gagaaaacgc tggctgatac
    18301 gttcttgatc caggcgctgt gccagttcat cacgcaatag tgacagcggc accgcgtcgc
    18361 catactgcgc acctaaacct tcggcgataa tcgcctgcca ttgttgttcg atcagcgtca
    18421 tcgccgcttc ggtttccgca tccggcagga agaaggcgtt gagcatatcg cgacaaaccg
    18481 gcaaccactc ttccagcgga cgctcctgtg ccagcccgcg acgccagatg tttagctgca
    18541 ttagcaggga agccagatgc cccaccagtt ctgcaattaa gccgctcgat tcatcataag
    18601 gtagaaccga ttgccactcg ccctgcgcgc tctccatcgc gtaacccagc aacatgcgcg
    18661 tcaggccaaa ccgccaggtg tgttgaccgg tagcggggag ttccagctcg cgaacgttgt
    18721 cgtcatctat cccccaacga atgccggatt cgttgaccca ctggcgtaaa taacgcagcc
    18781 cttcttcggt gatgtcaaac cgcgccgcca gcaccggcac atccagcagc gccagcacat
    18841 cctctgacac aaagcgactg tcaggcagtg ataacaggct gataaacgct tccagtaccg
    18901 gatgtgactg ccgcgcacga cggtcggaaa tggcgtaagg caggtaacga tccgcaggtg
    18961 cgctaccaaa cactgcctga ataaacggac tgtagctgtc gatatcagcc accatcacga
    19021 tgatgtcgcg cggagtaagt gtcgggtctt cctccagcat cgccagcagg cgatcgtgta
    19081 aaacttcaac ttcacgctgc gggctatggc aaacgtggaa ggtgatactg ctatccagtg
    19141 gatcaagcgg gcgtttgtta tcgctacggg aaaactcttc gatgttcaca ccagcaacgg
    19201 cgcggttttc cagttccaga atgtcagact gaatgttatg cagcaggtta tctggcatca
    19261 catcgacaaa ggcgtccagc tcctgactgc tatccagatc ggaaaggaga tagatatagt
    19321 cgcgccccaa cttgccccag gaggccagta gcgggttgcc gacatcctgt tcaccgtcgc
    19381 tgttaaagag ctgtccggca ttttcgctat cgcgaaatag cggcaattcg cgatcttcaa
    19441 aactgtgtcg acgctgacga gtcagcagtt tcgccagata agcaggatct ttaatatcgc
    19501 cccagtaata acggcagggg ttggtaaaca ggagatggat ttcaatatgt ttacccagcg
    19561 cctggagcgc ctggagataa acaggcggta acgcggaaat accgcatata aagacgcgcg
    19621 aaggtaaccc cggcgggcag gtcgtcgcgg actccagcgt ttcgataaag cgctgataga
    19681 gattggcgcg gtgccagcgc ggttgcccga gttgatgggt atattccacc aacgccttcc
    19741 acaacggcgc ttgccaggcc tgtgcttctc ccagcccttc aaccaaatgt cctgtttccc
    19801 actgtgccag ccagtccgga cgatagacca gatactggtc aaacaggtcc gccgcttttg
    19861 aagaaagctg gaacagtttt cgcttgtcgc tatcgtcagt cagataatgc cgcaacaggg
    19921 taaagtcttc gcgatccagc aattgcggca gcagagtcat cagtttccag ctcatgctct
    19981 gtttgttaaa ggcgctctct ttggggattt ccggtaacac ccggacgaac atatcccaga
    20041 taaagctcgc aggcagcgga aaatcaatgt ttgccgcaat accaaacttt tgcgacaggg
    20101 tcatttgcag ccactgcgcc ataccggtac tttgcaccag aatcatctct ggttcgaaag
    20161 gatcgtccag ccgttcgcgt tcgacaataa actccatcaa cgcttccagc acgtccagac
    20221 gattggaatg gtagaccctt aacatagcgg ctcctgacta ctgacgattc gggcaatgca
    20281 ggcgggtcat ctcgccttct ctgcccccgg gtgaaactag cgtaacgctg atgctgacac
    20341 atcccgcctg cgatgtctgc attcggttga cctgccagtt ggcaggtggc gaaatcgcgc
    20401 gcagttgcgt ttgctgccag ccatgccgcc agagctgttg gtactggttt ctgctggcaa
    20461 gactgttcat taatgttcgc tggaaacccg ataacgcagt gacaatcatc accatcaaca
    20521 ccatcgccaa cattacctcc ggcaggctaa agccttgttg attcttcagg gaagctgaca
    20581 taacgccctc tctttcagcg gacaaaaatc gctccagccg cgtggcgaaa aaacaatgtt
    20641 tccatcaatg acttcgcctg ttcgccacaa cgaaacgcct tcataaccgg caaccaataa
    20701 ggcttcatta tctgccagta aacgcaaaca aacccgggca tcggttccag cgtactgcga
    20761 gcactgaact gcgggctgcg tctgccagga gtgcattttc ccccacgcca gcgccgactg
    20821 aacgatggcc tggcggcgca atgactgact ttccatgctc acgcgagagg caaaactgcg
    20881 atcctgctga ctcattcctt gcaatagcaa gctacccaaa accagcagca tcagaaccag
    20941 agccagtgac gaaacacctt tttcgcggtt cacaggttga atcctgtcac gctatagctg
    21001 gcatccacca cggtttgcgg ttcagactta ctggcagcac gcatattaac cgtcaacacc
    21061 ggcgagaagc cgctgacatc ctgacgtacg acctgaaaag tgtcgataat gatggcatcc
    21121 ggattagtga ctttatccca gcccttacct tcacaggatg tcgcaccgcg tagcgtttcc
    21181 agcacatgct ccttcagacg aaatccaatc tggtcagact cttttaccgg ttcgcgatcc
    21241 cagataccgt tactgttcgc atcccactgc acaatgatac agtcaccctg tccgacaatt
    21301 tccagccctt cgccggtaca gctgccatga caataacccg ccctctggag atgcttcgcg
    21361 acggtaaata cccgcagcca gatttcatct tccagcgcca gcttacgggt gctcgttaaa
    21421 ctttcacgct gtaacgcagg cagaaagcgt gcagccccca gcaacaatac gctactgatc
    21481 gccatagcaa tcaacacttc cagcagagaa aaaccttgct cttttacagg catccttctg
    21541 tttctccttg ctgacaaagc cggagtcttc cccacggcga aaccaccagc caccactcgc
    21601 ccgttgagtt tttgaagcga atatgcccgg cccatgcggt attgcgcagg ccaaagaaag
    21661 caagcgaagg tgtcaggtcg ctcatttcga cttcgggcca gcgtggcaca aagaccaatg
    21721 gtgaactgcc atgacaggta ttggccccag cagcggaact cacaaggcac cataacgtcc
    21781 cctccctgat aacgctgata ctgtggtcgc ggttatgcca gttggcatct tcacgtaaat
    21841 agagcaaata gtcccgcgcc tggctggcgg tttgccatag ccgttgcgac tgctgccagt
    21901 attgccagcc atagagtcca cttgcgctta gcatgaccaa aatcagcatc gcgaccagcg
    21961 tttcaatcag cgtataacca cgttgtgttt tcatgccggc agtatggagc gaggagaaaa
    22021 aaagacgagg gccagtttct atttcttcgg cgcatcttcc ggactattta cgccgttgca
    22081 ggacgttgta aaatttcggg aaggcgtctc gaagaattta acggagggta aaaaaaaccg
    22141 acgcacactg gcgtcggctc tggcaggatg tttcgtaatt agatagccac cggcgcttta
    22201 atgcccggat gtggatcgta gccttcaatc tcaaagtctt cgaaacggta gtcgaagatg
    22261 gattcgggtt tacgtttgat aatcaacttc ggcagcggac gcggttcgcg gcttaattgc
    22321 agatgagttt gatccatatg attgctgtac aggtgcgtgt cgccccccgt ccagacaaaa
    22381 tcgcccactt ccagatcaca ctgctgcgcc atcatatgca ccagtaacgc gtagctggca
    22441 atgttaaacg gcaggccgag gaaaacgtca caggagcgct gataaagctg gcaagagagt
    22501 ttgccgtctg ccacatagaa ctggaagaac gcatggcacg gtgccagcgc cattttatcc
    22561 agttcgccta cgttccacgc tgaaacaata atgcggcgcg aatccgggtc gtttttcagc
    22621 tggttcagta ccgtagtgat ctggtcaata tgacgaccat ctggcgttgg ccaggcgcgc
    22681 cactgtttac catacactgg tccgaggtcg ccgttttcat cggcccattc gtcccagatg
    22741 gtgacattgt tttcgtgtag ataagcaatg ttagtgtcgc cctgcagaaa ccacagcagt
    22801 tcgtggatga tggaacgcag gtggcaacgt ttagttgtca ccagcgggaa tccatcctgc
    22861 aggttaaaac gcatctgatg accaaaaatg gaaagcgttc cggttccggt acggtcgttt
    22921 ttctgtgtgc cttcgtcgag cactttttgc atcagttcta aatactgttt catggttcct
    22981 caggaaacgt gttgctgtgg gctgcgacga tatgcccaga ccatcatgat cacacccgcg
    23041 acaatcatcg ggatggaaag aatttgcccc atgctgatgt actgcaccca ggcaccggta
    23101 aactgcgcgt cgggctggcg gaaaaactca acaatgatgc gaaacgcgcc gtagccaatc
    23161 aggaacaagc cagagacagc tcccattggg cgcggtttac gaatatacag gttgaggata
    23221 ataaacagca ccacaccttc cagcagcagc tcgtaaagct gtgatgggtg gcgcggcagc
    23281 acaccgtaag tgtcgaaaat ggattgccac tgcgggttgg tttgcagcag caaaatatct
    23341 tctgtacggg agcctgggaa cagcatggca aacgggaagt tcgggtcaac gcggccccac
    23401 aattcaccgt taataaagtt gcccagacgc ccggcaccaa gaccaaacgg aatgagtggt
    23461 gcgataaaat cagagacctg gaagaaggaa cgtttagtac ggcgggcgaa gataatcatc
    23521 accacgataa cgccaatcag gccgccgtgg aaagacatgc cgccgtccca gacacggaac
    23581 agatacagcg gatcggccat aaactgcggg aaattgtaga acagaacata accaatacgt
    23641 ccaccgagga agacgccgag gaagcccgca tagagtaagt tttcaacttc atttttggtc
    23701 cagccactgc ccggacgatt cgcccgtcgt gttgccagcc acattgcaaa aatgaaaccc
    23761 accagataca tcaggccgta ccagtgaagc gccacgggtc ctattgagaa aatgaccgga
    23821 tcaaactccg gaaaatgcag atagctactg gtcatctgtc accacaagtt cttgttattt
    23881 cgctgaaaga gaacagcgat tgaaatgcgc gccgcaggtt tcaggcgctc caaaggtgcg
    23941 aataatagca caaggggacc tggctggttg ccggataccg ttaaaagata tgtatatgat
    24001 ccgcgctata accctccgcg aatcagcccg cccatgccgc gacgctccat aaaggctgca
    24061 acctgatggc gaacttcggt cgccagttgc gcttccagac tacgctgcgc aagattttct
    24121 gcgtcggcat aatcaatgcg ccgcagcagg tattttaccc gcgctacaga acgtccgttc
    24181 atagacagat ggcgataccc aagcccaatg aggattgcca cgcacatggg atcgcccgcc
    24241 atttcaccgc acaaacggag atcgattcca tgtatttccg cttcccgggc gatcatcgcc
    24301 agagctcgta acattgcagg atgaagactg tcataaatgt tcgccacccg ggtattgttg
    24361 cgatcaacgg ccagaatgta ttgagtcaga tcgttggtgc caacagagat gaaatcgacc
    24421 cgctttgcca gatgcggcag cataaatacc attgacggca cttccagcat gatgccgata
    24481 cgtggtttgg gaatttcgta accgatcatc tcctcgactt cacgtccggc acgttcaatc
    24541 aggcggcgtg cttcatcaac ttcatcgagg cttgtgacca tcggcaacag aatattcagg
    24601 ttgcccgtag cggcattagc acgcagcatc gcccgcacct ggatcaagaa gatctccggc
    24661 tgatcgagcg taatgcgaat cccacgccaa cccaggcatg gattctcttc gctgatcggc
    24721 atgtaaggca gctgcttatc tgctccgaca tccagcgtac gcaaggtgac gggtttatca
    24781 ttgaacattt gcagcatccc ctgatactgc gccacctgtt cttcttccga cgggaaacca
    24841 ctttgcagca tgaatgggat ttcagtgcga taaagaccga tgccatcaat acggctgccc
    24901 agtttttctt catgttccgg gcttaaacca gcattgagca tgacttttat acgctcaccg
    24961 ctttttaact gcgcgggtaa attgacgtca tcttccgcca gacggctaag ctcaatctct
    25021 tcactaatta gccgctgata ttcttgcagc agtaccggct ccggatcgac cagcaattca
    25081 ccgcgatagc catcaacgat cagcgtccta cgatgcagca ccgaaggctg aatatccgcg
    25141 cccatcacgg tagggatccc cagcgcacgt accatgatcg cagcatggga gttggctgcg
    25201 ccatctcgca cgacaacacc gactaagcga tcctggggca gctcagcaag agttgtcgct
    25261 gacagttcat ctgccaccag aatgaaacgt tccggccagg cgttcggccc ttgattagcg
    25321 tcatcaagat gaaacagcaa tcgctgaccc agcgcacgta aatcgccagc ccgctctttg
    25381 agatagttat cgcttagcgc ggcaaactgt tcggcaaatt tttcaatgac cgtttttacc
    25441 gcccactctg ccaccgagcc tttatcaacc tcggcaaaca attcgcgacg cagccgggtg
    25501 tccgaaagca ggtgcgagta aagatcgaaa atagccgccg tttctttttg tgctccggcg
    25561 gcaaagcgtt tgctgtagcg gcgaaactcg tttgccgctt cttccagcgc cccggtcaat
    25621 cgttcgcgtt ccagagccgg atccagcgtt gatgcctgat acacctgttc cattaaaggt
    25681 aacgtggcat cctgccagcc ttcggcaatc gccacaccag gtgctgccgg taatgcgcgg
    25741 attcgcgtct ggcgatattg cccaaacaag gcagtcaact gcgactgaga aagaatagct
    25801 gccatctggg tggcaagcgt caccaggaag gattcttcac tttcgtcata ctggcgcaac
    25861 tctcgttgct gtaccaccag tacaccaagc aactggcgac gttgaataat tggtacgcct
    25921 aaaaacgcgc ggaaacgttc ttcttttacg gaggggatgt atttgaagct ggggtgcttt
    25981 tgcgcatctg caaggtttat cggttccgcc agcctgccaa ccaggccgac gatcccttca
    26041 tcaaacgcga gcgttacagt gcgaccgcgt ggttttttca gcccccgggt cgccatcagg
    26101 tagtaacaac gtcgatcatg atcggccagg tagaccgaac agacctcggt atccatcgca
    26161 agacagatgt cggtaaccag aatatttaac gcctcattca ggcgtggtgc gctggctacc
    26221 ttttcgacta tttcgcgcag gcgagtgagc ataattggcg tgacttaacc tcttttacgt
    26281 cgataagcag atgcgttttg tggttttggc gtattttcct gcagtgacat caccacactc
    26341 gcgaactctt tcattaccct acggtagaca tcacgtttaa atgacaccac ctgtctgacc
    26401 ggataccagt aacttaccca tcgccagccg tcaaactctg gtgtactgct ggtttgcata
    26461 ttgatttctg catcgccgct caccagctgc aagagaaacc atttttgttt ttggccgata
    26521 caaaccggct tcgtgtccca acgcaccaaa cgtttcggta atttgtagcg caaccagtta
    26581 cgcgttgaag caaggattcg aacgtctttg cggcttaatc ctacttcttc aaacaattca
    26641 cggtacatcg cctgctctgc ggattctccg gggttgattc cgccttgcgg aaattgccag
    26701 gagtgctgac caaatcgccg ggcccacatt acctgcccct ggcgattaca aatcacgata
    26761 cctacgtttg ggcggtagcc atcgtcatca atcaccggac tacctcaaaa taaagcttta
    26821 tatacgaatg attgtttcat actccaggaa gacggtaaac cactctctgc agggcattac
    26881 acactaataa caattgaata actcacagtt atgtgcagag ttataaacag aggaaggggt
    26941 ggatagccgt ttttattcac cttttctgtg gatagagttg tgaagaacta cggaattact
    27001 acgggaaaac ccggagcatt ctgaataagc ccgatataga cattttaaaa tattcattat
    27061 tagtcaatgc attacatgta atttgagtaa tgaaaaagag taattcgtga cccaggtcac
    27121 acctctcatt tacgggttgg cgaaagatca accaatgccg tatttatcca cagaatgtgc
    27181 cactaagtta agcactgaac cactaaaaac tggagtttcg tcgcacgtca aggctgtaaa
    27241 tggaaacagt agtggaggtt tttcacagtt atcccagctt tctgtggata acatggtgta
    27301 agatcctgtt tattttcagt gaccagattt ggaaaacccg ttgcagtgtt gcgcaactcg
    27361 attaccggca gcctaaaaag caatataaat cagggaattg aacaacacat gtggaaaaag
    27421 ttacactgcg aatattcggc acataattgc tgtttgtttt ttaatcaagg tatcatgaca
    27481 tgtcccaacc tcgcccactg ctctctcctc ccgaaactga agaacagttg ttagcgcaag
    27541 cacagcaact ttctggttat acattgggag aactggcggc acttgccggg cttgttacgc
    27601 cagagaattt aaaacgcgat aagggctgga ttggcgtgtt actggagatc tggctaggtg
    27661 ccagcgcagg gagtaaacct gagcaagatt ttgccgctct gggcgtggaa cttaaaacta
    27721 tccctgtgga tagtcttggt cgtccgctgg aaacaacatt cgtttgtgtt gccccgttaa
    27781 cgggtaatag cggggtgacc tgggaaacca gccatgtgcg ccacaagctc aaacgcgtac
    27841 tgtggatacc ggttgaaggc gagcgcagca tcccgctggc gcagcgtcgc gtaggatcac
    27901 cgttgctgtg gagcccgaat gaagaggaag accgacagct acgcgaagac tgggaagaat
    27961 taatggatat gattgttctc ggtcaggttg agcggatcac cgctcgtcac ggggaatatt
    28021 tacagatacg accgaaagca gcgaatgcga aagcgcttac cgaagccatt ggtgcccggg
    28081 gcgaacggat tctgacgctg ccacgcggct tttatttgaa gaagaatttc accagtgcac
    28141 tactggcccg tcattttctg atccagtagc catcgctttg acctgccgct ttccgggcat
    28201 ataattaccg cttcattttt ttggcagggc tttttagatg ttatttgcat ggataaccga
    28261 tcctaacgcc tggcttgcgc tcggtacgct gacgctgctg gagatcgttc ttgggatcga
    28321 caatattatt ttcctttctc tggtggtggc aaagcttccc acagcacaac gtgctcatgc
    28381 gcgccgtctg gggttggcgg gagccatggt tatgcgtctg gcgctgctgg catcaatcgc
    28441 ctgggttacg cgcctgacaa atccgctttt tacaatattc agtcaggaaa tttctgcccg
    28501 tgatttgatt ctgcttctgg gtggcttgtt ccttatctgg aaagccagca aggaaatcca
    28561 cgaatccatt gaaggtgaag aagaagggct gaaaacacgc gtttcatcat tcctcggcgc
    28621 tatcgtgcag attatgctgc tggatattat ctttagcctc gactcggtga ttacggctgt
    28681 gggtctgtca gatcacctgt ttattatgat ggcagccgtg gtgattgccg taggcgtgat
    28741 gatgttcgcc gcgcgctcga ttggtgattt tgtcgaacgc catccttcgg taaaaatgct
    28801 ggcgctctct ttcctgattc tggtgggctt taccctgatt ctggaaagtt tcgacatcca
    28861 cgtaccgaaa ggttacatct acttcgcgat gttcttctct attgcggttg aaagcctcaa
    28921 cttgattcgc aacaaaaaga atccgctctg atactccgta cgctctcctg cgggagggcg
    28981 tactcactcc ttttaccctc acagtttaag attttcctgc tttcaagata tataacgtcg
    29041 gtttataaac agactattat cataggtagg ccaaatgagg ataaccggat gaaaaaatgg
    29101 gcagtaataa tttctgcagt cggactggcg tttgctgttt ccgggtgttc caatgattac
    29161 gtcatggcga ccaaagatgg ccgtatgatt ttgaccgatg gaaaacctga aattgatgat
    29221 gataccgggc tggtgagtta tcacgatcag caaggtaacg cgatgcaaat taaccgtgat
    29281 gatgtttcgc aaattattga acgttaacaa ataaggtcag catccggctg gccttaagat
    29341 ttttctcttt cccttttccc ttccctctgc catttttata ttccttatgt cgtgattata
    29401 aaaaggaaac ggctatgcaa tatcaccgta taccccacag ttcgctggaa gtcagcacgc
    29461 tggggcttgg cacgatgacg tttggtgaac agaacagcga agccgacgcc cacgcacaac
    29521 tcgactatgc cgtcgctcag ggcattaacc ttatcgacgt tgccgaaatg tacccagtac
    29581 ctccgcgccc cgaaacgcaa gggttaaccg aaacctacgt cggcaactgg ctggcgaaac
    29641 atggcagccg cgaaaagtta attatcgcct ccaaagtgag cggaccgtcg cgcaataatg
    29701 acaagggcat ccgcccggat caggcgctgg atcggaagaa tatccgcgaa gcgctgcatg
    29761 acagcctcaa gcgcctacag actgattacc tcgatcttta tcaggtgcac tggccgcagc
    29821 gcccgaccaa ctgcttcggc aaactcggtt atagctggac ggattctgcg cctgcggttt
    29881 cgctgctgga tacgctggac gcactggcag agtaccaacg cgcgggaaaa attcgttata
    29941 tcggcgtgtc gaacgaaact gcatttggcg taatgcgcta cctgcatctg gcggacaaac
    30001 acgatctgcc gcgtattgtc accattcaga acccttacag tctgttaaac cgcagttttg
    30061 aagtaggtct ggcagaagtc agccagtatg aaggggtcga actgctggcc tattcgtgcc
    30121 tgggtttcgg cacgctgacc gggaaatatc tcaatggtgc aaaacccgct ggcgcacgta
    30181 atacgctctt tagtcggttc acccgctata gcggtgagca aacgcaaaaa gccgtcgcga
    30241 cgtatgttga tatcgccaga cgtcatggcc tggaccctgc tcagatggcg ctcgcgtttg
    30301 tacgccgtca accgtttgtt gccagcactc tgctgggcgc aaccacgatg gatcagctga
    30361 aaactaacat cgaaagtttg catctggagt taagcgaaga cgtattagct gaaattgaag
    30421 cggtgcatca ggtttatact tatccggcac cataagatta acgccggtta aaaccggcgt
    30481 taaatattaa tgacggcgct gccagatcca cagcgccgtt attgccagcg caaacagcgc
    30541 accgaagcca atgccaatgg gcacgaccgg gatgcctatc attaccgcca gcgagtaaat
    30601 gcccagcatc aacaacatgg cgctgttttc gccaaggttt tgtactgcaa tcgcattccc
    30661 cgccccgacg ctttttttac cccgctcctg tagcaacgca ttgagcggaa cgacaaaaaa
    30721 gccccccatc acgccaatca gcatcaacaa ggcataggct ggcagcagct cgtgttgcag
    30781 ggaaaaaatc agtaccacca cgccaatcaa aatcccggct ggcatacagc gtgacacggt
    30841 ttccagcgta actaacttcg ctgccgcacc tgcgccaacc acgataccaa tcgctaccat
    30901 cgcgttgaga taggtgggcg tagcgttatc ggtaatgccc agcgccaccg gtacccacag
    30961 caccaacagg aaacgcagcg tgacacccgc tccccagaat aaactggtgc ccaccagcga
    31021 aaaacgcgtt tcaccattgc gccatagcga ggtgcaggca ttcaggaaac tgcgggtcat
    31081 gttgatgaga ttccaggact gccccggacg cgccgccgcc agtttgggaa tgtagatatt
    31141 ggcaacgacc gcaccaccgt aggccagtgc gcatgcggcc agggcgacga ggacatgcca
    31201 gtcagccagc acaccaccgg ctacggaacc gagcaaaatc gccgctatgg tagaagcttc
    31261 cattaaaccg ttagctttca ctaacttact acccgtggtt aattcgccga gaataccgta
    31321 tttcgccggt gaataggctg cagcaccaac acccaccagc gtatagccga gaaacggatt
    31381 gataccaaag cagatactgg ctgcgcccag cagcttcagg ccgttggcaa acatcatcac
    31441 ccggcctttg gcgaagctat ccgccacctg cccgacaaac ggcgcaaaaa gaatgtaagc
    31501 acctacaaac accatttgca ggatgggctg gctccactcc ggatagaact gcgctttcag
    31561 taacgccaga gtggcaaaca gtagggcatt atcgccaaac gcagagagaa actgcgccac
    31621 gataaccgct ttcatcccct tcgaccacaa cgaagtgtta gtgtgcactg actcactcat
    31681 cgtgttgttc cgcttcgtct acccagcttt tcaacgtgac aaagtcaggt ttgccgctgc
    31741 caagtaatgg catctgtttc agatagcgaa tatcgcgcgg tacagcaagc tccggcacgc
    31801 cgtgctcgcg ggcatactgt tgcaacttat cgcgcgtcag ttcgttatct gtggtgaaaa
    31861 gcaccagtgc ctcgcctttg ctggcatcgc tcttaatcgc agtggcatgg actttatctg
    31921 gcgaaacacc aagtgccagt tgttccacca tttccagcga caccatttcg cctgcaattt
    31981 tggcaaagcg ttttgcgcgg ccctgaatct gcacaaagcc ctgctcgtca aaacgcacaa
    32041 tatcgccagt gtcataccag ccgcgctcca tttcgccgcg aacattctcg gcggtgggca
    32101 cttccagtac acctggcttc tccacccgca gatagccgtt cattatgttc ggccctttca
    32161 gttgcaggcg tccgccctct tcgataccag gaaccgacaa caggcgcgca tccattcctg
    32221 gtagaatacg ccctaccgta ccgggtttcg ccgccatcgg tacgttgata gaaacgacag
    32281 gcgcgcactc ggttacacca tagccttcaa ggatgcgcag gccaaattta tcctgccaaa
    32341 gctgtttggt actttcttgt agtttttctg cgcctgccac cacatagcgt agacgataga
    32401 agtcatacgg gttggcgaaa cgcgcgtagt gaccgaggaa agtcgaggta ccgaacaaca
    32461 cggtgcaact gcggtcatac accaactccg gcacaatgcg gtaatgcagc gggcttggat
    32521 aaaggaacac ttctgcacct gtaagcagtg gcgtaaacag gcctaccgtc agcccaaagg
    32581 agtgaaacag cggtaatgcc gacataaagc gatcgttggt ggtgaagtcg gcaatcgttt
    32641 taatctgctc gacgttcgcc agaatgcttt tatggctatg gacgacgcct tttggatggc
    32701 cttcagaacc ggaggtaaag aggatcagcg cctcttcttc cggctgctgt tttacctgcg
    32761 ccaggcgcgg catcagcaaa tgagcgaaga tccatacttt gtcggcagtg gtgacatctg
    32821 cttttaaatc ttccagatag acccagcgca cctgagtaag ttgctccggc agatgccaga
    32881 gtttgccttt atcgagaaac tggcgggaag tgaagatggt tttgatttca gccgccgtaa
    32941 tagcactggt cagccctttt accccagcag tgtagttcat cattgcggga atgcggcgac
    33001 gcgcgatggc cccaaaaatc actgccgcac tgatgcctgc attgggcagc attaagccga
    33061 tgcgttcgcc ttcaacactg tatttttcaa ggatgcgtcc aacaaacagc gttttcgtaa
    33121 gcaatttgcg ataggagtct ggggtaaagt tgacgtcttc gacacatttc ttcccggctc
    33181 cgaagcggta cattgcactc agtaaagatt cgtacagagt ttcacgcggg cgcaccgcca
    33241 tacgcgcttc catcattatt tgatgcagca tttcgccagc gattttgcga cggtcacggg
    33301 cacgcggcgc atccggcatc tccacctgcg ttggtggcaa aatatgcaga gtaatttgcg
    33361 ggaacaagcg acgtttaacc agacctttca ggcggctgaa gtgcgtaagt tccgcccctt
    33421 caatacgcac aggaataacc gttgcaccag acttcgccgc gacaaaaccc gcgccatcgt
    33481 agattttcat cagcgagcct gtagtggtga tgcgtccttc agggaaaatc accactggtc
    33541 ggccctgttc caccagacgt accagatgtt taatagccat aggttgcgtc gggtcgagag
    33601 gaacaaagtc gataaatgat ttcagccaac gcatatacca ctgttggctt attgaggtgt
    33661 aaacggcaaa cactggacgc acaggtaaaa acagtccaag caaaatgcca tcaataaaag
    33721 agacgtgatt aggcgtaatt agaacgcgct cgcccttcag tgcctgggtg tcacccgtaa
    33781 cgcgaacgcg atacaaaaca cggcacaaat ttcgaaaaaa gctaaaaagc attccaactc
    33841 cctttgctct gattcagtaa aagcgaatgg agggagatta cacgagataa agaacgcgag
    33901 cgacagtaaa ttaggtgcga aaaaaaacct gcgcatccgc gcaggttggt gcaagagaca
    33961 gggtacgaag agcgtaccgc ataatctcac caatcaatac ctctgggatc ttgattgtgg
    34021 tctgcacgac gactcttcgc cagcgagaaa acgcaaagga atgaagggaa atgcaacgag
    34081 gtgtgtaaat tgtcggttac tgttacagat tgatgaccgg ccaaaaaaaa cctgcgcatc
    34141 tgcgcaggct ggtgtaattc atgtgctcaa cccgaagttg acttcaccta tcaatacctc
    34201 tgggatcacc actttagcaa cctgaagcca aacgccacca gcggtcaatc gcaacagcgt
    34261 ttcgcaaagt gtaaccaaag gtttgcaatc tctttttttg tcttacttat ttgcgtttgg
    34321 ctcacattcc cacgatgaaa acacgccacc ccttgaacca acgggcgttt tccgtaacac
    34381 tgaaagaatg taagcgttta cccactaagg tattttcatg gcgaccataa aggatgtagc
    34441 ccgactggca ggcgtttcag tcgccaccgt ttcccgcgtc attaataatt cacctaaagc
    34501 cagcgaagct tcccggctgg ctgtgcatag tgcaatggag tctcttagct atcacccgaa
    34561 cgccaacgcc cgtgcgctgg cgcagcagac cactgaaacg gtcggtctga tcgttggtga
    34621 tgtttccgat ccgtttttcg gtgcaatggt gaaagcggtc gaacaggtgg cttatcacac
    34681 cggtaatttc ttattgattg gcaacggtta ccacaacgaa caaaaagagc gtcaggctat
    34741 tgagcaactg atccgccatc gctgtgctgc gttggtcgtc catgccaaaa tgatcccgga
    34801 tgccgattta gcctcattaa tgaaacaaat gcccggtatg gtgctgatca accgtatcct
    34861 gcctggcttt gaaaaccgtt gtattgctct tgacgatcgt tacggtgcct ggctggcaac
    34921 gcgtcattta attcagcaag gtcatacccg cattggttat ctgtgctcta accactctat
    34981 ttctgacgcc gaagatcgtc tgcaagggta ttacgatgcc cttgctgaaa gtggtattgc
    35041 ggccaatgac cggctggtga catttggcga accagacgaa agcggcggcg aacaggcaat
    35101 gaccgagctt ttgggacgag gaagaaattt cactgcggta gcctgttata acgattcaat
    35161 ggcggcgggt gcgatgggcg ttctcaatga taatggtatt gatgtaccgg gtgagatttc
    35221 gttaattggc tttgatgatg tgctggtgtc acgctatgtg cgcccgcgcc tgaccaccgt
    35281 gcgttaccca atcgtgacga tggcgaccca ggctgccgaa ctggctttgg cgctggcgga
    35341 taatcgccct ctcccggaaa ttactaatgt ctttagtccg acgctggtac gtcgccattc
    35401 agtgtcaact ccgtcgctgg aggcaagtca tcatgcaacc agcgactaac cgcagttaaa
    35461 gcaattccag cgccagtaat tcttcgatgg tctggcgacg gcgaatcaac cgcgcctgac
    35521 cattatcaaa cagaacttct ggtaacagcg gacggctgtt gtagttggat gacattgatg
    35581 cgccatatgc ccctgtatca tgcagtacca gataatcacc cgccttcact tccggcaagg
    35641 cgcgggtttc aacatttccc ccttcctgct gggtaaagac atcgcccgat tcacataatg
    35701 gcccggcgac gacggtttcc accgttggca cgtgttccag agaacgacca tcagctgcca
    35761 gggcactgat atggtggtaa ctaccgtaca ttgccgggcg catcagatcg ttgaacccag
    35821 catcaaccag cacaaagtgg cggctaccca tttgtttgac gctccgcacc tgagtaatta
    35881 atacgccaga ctgcgctacc aggaagcgac ccggttcaat ttccagtttc acagggtggc
    35941 ccaaatggcg ggcgatttgc tcacgcgcgg cattccacag accataataa tgttcggtat
    36001 caaccgcctc ttcaccctgt tgataaggaa cagaaagccc accgcccgca gaaatagcct
    36061 gtaaatcctg accgaattcg atgacctgac gcaccatagc accacacacc tgttccagat
    36121 gggcataatc aacgccagaa ccaatgtgca tgtgaatgcc gaccagctgc agatgatgac
    36181 gttgtatcac gtccagtgcg gcgggcagat cggtgtacca gataccgtgc ttgctgtttt
    36241 cgccaccggt attggttttt tggctatgtc cgtgaccaaa ccccggatta acgcgcagcc
    36301 atacccgatg ccctggcgaa acctggccca gttggtcgag catatcaaca gaacccgcat
    36361 tcaccggaat ttgcaattca ctgacgcgtt caagcgtcgc ctgatcgata acatctgccg
    36421 taaaaacaat atcatcgggg tgcgtttgcg gattgtaacc cgccgccaac gcacgctcta
    36481 tttcgcctaa cgagacggaa tccactttca cgccctgctc acgcattaag cgcaaaatat
    36541 gaatattgga acaggctttc tgtgcaaagc gcaccacatc aaactgtttc agcgctgcaa
    36601 tctgccgacg aataatttgc gcatcgtaga cccacaccgg gcagccaaat tcagcgggca
    36661 aacgcagcag attttcggcg gtgagatcgg tatcggtgct gaacagtgaa tgtggcatag
    36721 caaactccag ataagtgctt tttttatgat tacgccacat cataaaaaga ataaaaaata
    36781 tcgatttatg tcgagtctat gcaaaaatga tatggattac cggattgcga gagagcgcta
    36841 atggccgccg ttaacttacg tcatattgaa atttttcatg cggtaatgac cgccggaagt
    36901 ctgactgagg cggcacacct gctacacacc tcacagccaa ccgtcagccg cgaactggcg
    36961 cgctttgaga aggtgatcgg gctgaaattg tttgagcgcg tacgtggacg attacatcct
    37021 accgtgcaag gactgcgtct gtttgaagaa gtgcaacgat cctggtacgg actggatcgc
    37081 attgtcagcg ccgcagaaag tctgcgcgag tttcgccagg gagaactgtc tattgcctgc
    37141 ctgccggtct tttcgcaatc ttttttaccg cagctcctgc aaccctttct ggcacgttat
    37201 cccgatgtca gcttaaatat cgtgccccag gaatcaccgc tacttgaaga gtggctctcg
    37261 gcccagcgtc atgatttagg actcactgag acgctccata cgcctgcggg aacagaacgt
    37321 accgaattac tctctttaga tgaagtgtgt gtgttacctc cgggtcatcc gctggcggta
    37381 aaaaaggtat taacgccgga tgattttcag ggtgagaact acatcagcct ttcccgtact
    37441 gacagctatc gccagttgct ggatcagcta tttactgaac atcaggttaa acgacgcatg
    37501 atcgtagaaa cccacagcgc cgcgtcagtc tgcgcaatgg tacgggcggg ggtaggtgtt
    37561 tcggtggtta acccgctcac cgcactggat tatgcggcaa gcggtttagt ggtgcggcgg
    37621 ttcagtattg cggttccgtt caccgtcagc ctgatccgcc ccctgcaccg cccgtcatca
    37681 gcgctggtgc aggcgtttag tgagcattta caagcggggt taccgaaact ggtcacttct
    37741 cttgacgcta ttttgtcgtc agctacgaca gcataaaagc gacagcatcc tcggcatgga
    37801 tcgccgcagt atcaaacaca ggcaaaacac tgcgctcttc tggcaccagt aaaccaattt
    37861 ctgtgcagcc aaaaatgacg ccctgtgcgc cctgttctgc aaggcgagca atcacttgcg
    37921 cataataagc gcgtgacgct tcggtaaatt gccccagaca cagttcttca aaaataatct
    37981 gattaatttt cgcccgttca tccgcttcag gaataagaca attgatggaa aattgttccg
    38041 tcagccgccc gcgataaaaa tcctgttcca tggtgtaacg cgtacccagc agcgccacac
    38101 gagtcattcc ggccccggta attgcacgtc cggtggcatc cgcaatgtgt aagaaaggca
    38161 gagtgcaacg tgactcaatg gcatccgcca ctttatgcat cgtattggta cataacacaa
    38221 taccttctgc gcccgcccgc tgtaagccaa gcgccgcctc agccagaatg tccccggttt
    38281 tatcccattc cccgcgacgc tggcactctt ctatttcatg aaaatcgacg ctatgtagca
    38341 gcacttgcgc agagtgaagc ccaccaagcc gctgtttaat gccttcattt atcagacgat
    38401 agtaaggaat ggtggattcc cagctcattc ctcccagcaa accaattgtt ttcattcctc
    38461 tctcctgatg tgtgttaccg caacaaggct gaataaggaa cgagacaaac gcctcaacgg
    38521 ccaggtgccc aatctctatt aacgaaaaaa gggccggatg tacagcacat ccggcccgtg
    38581 aaatcagacg ccgatatttc tcaacttctc gcctgccatc agtttgcgtt cgatatgttc
    38641 cagcgtgaca tttttggttt ccggaatgag ccagaaagtg atgcccacaa acgcaatgtt
    38701 cagcgcagtg tagagccaga acgtaccggc agcgccaatg ctgtcgagca gcgtcaggaa
    38761 ggtcgcgccg ataatcatat tcgacaccca gttcgtggtg gtcgaacagg tgatgccaaa
    38821 atcgcggcat ttcagcggct gaatttcaga gcacaggatc cacaccactg gcgcggcgct
    38881 catcgcataa ccggcaatac acatcatcgt catgccaaca gagagccagg acaagccact
    38941 ggaagccgta ccgttatcaa actgcatcag gcaatagccc agcaccagag tgcctaacgc
    39001 catcacgctg aaaccaattt tcagagccgg tttacgacct gctttatcta ccgtaaagac
    39061 cgcaatgaag gtcgcgaaca taaaggtcag tcccacgacc agagtcgcaa tcatctgttg
    39121 ttctgtggtc gtaaagcccg ccattttgaa gatacgcggc gcatagtaca tgatgatgtt
    39181 cataccggta aactgctgca tcgcctgcaa caacataccg agaaacacag cacgacggac
    39241 gttacggttg atcttaaaca gtgcccaacc gccttgtttt aacttcaggc tttcacgaat
    39301 ttcgttgagt tcttctcgcg ctttttccga cgtatcgcgc agcatacgca acacttcttc
    39361 cgcctcaata tgacgcccct tttccgccag ccagcgcggg ctatttggca ggaagaccac
    39421 cagaataatc agcaggactg ctggtaaagc aagaaccccc aacattgcgc gccagttacc
    39481 gctataactg aacgctgtat cggataaaaa cgccagcacg atgccgagtg tgaccatcaa
    39541 ctggtacata ctgatcatct taccgcgaac gttttcactt gccatttcag aaagatacag
    39601 aggagcggtg taagacgcga tcccgacagc aatgcccagc accacacgag cggcgattaa
    39661 catctctacg ctggtcgcaa aagcggaccc tatagaaccg agtacaaaca ggatggcccc
    39721 cgccatcagg ctgtatttac gacccaggcg gaacgacagc caaccattaa acagcgcacc
    39781 aattgctgcg ccgagcatca tgctactaac cacccattcc tgcaaacgac tggtcagcac
    39841 aaagtgatcg gtaatgaacg gcaacgctcc ggcgattacg ccgatatcaa gaccaaataa
    39901 caatcctgcg accgcagcag ctaccgaaac aaacatattc atacgccgcg tatcccgcaa
    39961 agaacgtggc gttaaagcag attccgtatt gatagtaacc attttttcct gccagcagag
    40021 agtaagacat agtgaaaaaa tacgtgaaca actcacgcag gtgtcaggtc ggaaacagca
    40081 taaatatgga ttaaattgct gcgacatgtc gttatgtgat ggatattcca attttcaaat
    40141 taagttgaat tattgagatt attattaacc acctaatttt acagcagata aaattcataa
    40201 agttcattaa ttgataatta atatggatta tttcataacc atgatatgga ttatgatgat
    40261 ctacaggtat aaaaaaccct gccatgcggc agggtcataa aagtaagaag aatgaattaa
    40321 cgcgccagcc aaccgccatc cacggcaatg gtataaccat tcacataatc tgaagcgctg
    40381 gaggcaagga acactatcgg ccccatcagg tcactcggca gtccccaacg accagctgga
    40441 atgcggtcga gaatttccgc gctacgttgt tcatctgccc gtagttgttg agtattgttg
    40501 gtcgccatgt aacccggagc tatcgcatta acattaatgt tgtgtttagc ccattcgttc
    40561 gccatcaatc gcgtcacacc catcacgccg ctttttgatg cggtataaga aggcacacgg
    40621 atcccgccct ggaaggagag cattgacgcg atattgataa tcttgccgcc attgccttgc
    40681 gcgataaagt gtttcgccgc tgcctgagac atgaagaata cgctcttgat attcaggttc
    40741 atgacatcgt cccagtcctt ttcgctgaac tcgagagcat cttcgcggcg aatcaatccg
    40801 gcgttattca ccaggatatc aatatgacca aactccgcta ccgcgcgatc cagcagtcca
    40861 gaaatgccat caatctttcg cagatcggcg gtcaggctta aaaaacgacg ccccagcgcc
    40921 gtgacctgct cgatggtttc agtcggttca acgatgttaa tgccaacaat gtcacagccc
    40981 gcttgcgcca gccccaacgc catcccctgg cccagcccag tatcacaacc agtgacgacc
    41041 gcaactttac cttcgagaga aaatgcactt aaaatcataa caatacctta ttcgtttatg
    41101 cccacaacta gcgcaaatct ttaacggcca catggtccat atcatcaaag acctggtttt
    41161 caccgaccat gccccagata aaggtataag ctttggttcc gacgccggaa tgaatcgacc
    41221 agctcgggga gatcaccgcc tgctcgttat gcatcacaat gtgacgcgtt tcttgcggct
    41281 gccccatcat gtggaaaacg caggcgtcat catccatatt gaaatagaaa taaacttcca
    41341 tccggcgctc gtgagtgtga cacggcatgg tgttccacaa gttacctgga gccagctccg
    41401 tcagccccat actcaattgg caggtttcca gcacatccgg gacaaaatat ttgttaattg
    41461 tgcgacggtt actggtgagg ttatcgccta acgtgactgg agatacttcg tccggtgtga
    41521 cttttttggt gggatacgtc gtatgcgcgg gtgcgcaatt gtaataaaac tttgccggag
    41581 tggcggtatc aatactggca aagacaactt cttttgcacc tttacccaca tacagggcgt
    41641 cgcggtgacc gatttcatag cattggccat cgacagtaat cgtaccggca ccgccaatat
    41701 tgataacacc taactcgcga cgttcaagga aatagcttac gccgagttgt ttaccaactt
    41761 ccccgccaac ggaaaccgtt ttagttatcg gcataatgcc gccaacaata attcggtcaa
    41821 tgtggctgta aaccatggtg tactcatcgg cgacaaatac ctcttcaacc aaaaattcat
    41881 tgcgcagccc ctgggtatcc agcgtttttg cgtgcgcact gtggatgctc tgtcttacgt
    41941 ccacatcaac ctccgataat cagtgaagcg tgaaaataga aaacgatagt gaaacactgt
    42001 ttcgttttct ctataagggg atgatatgcc catgggatcg ggttttcaat taaaaataaa
    42061 acaatgtttc atttttaagt taaggattaa aaaaagtgca gtgtcgatca cgaatctgtc
    42121 atgagaatgg aaaaatcagg gagcccggaa aatgtatgtc gacttgccag ccatcattat
    42181 tcaacagatt aatgacggca gatatttatc ggtaaggagg aaatgcagag ctattcgtca
    42241 cgttcaatgg tcaatgccac cccctggccc ccgccgatac aaagcgttgc cagtcctttg
    42301 cgggcattac gtttcaccat ttcatgaacc agagaaacca ggattcggca accggaagcg
    42361 cctatcgggt gaccgagtgc gatcgcgcca ccattgacat tgacccgacg ctcatcccac
    42421 tcaagcatct tgccaaccga aagcgcctgt gcagcaaacg cttcattagc ctcgataaga
    42481 tcaacatcag ccaactgcca gcctacacgc tccaggcaac ggcgggtcgc atacaccggc
    42541 gcaattccca tcaatgccgg atctacacca acgctggcaa atgcgcggat gcgggccagc
    42601 acgggtaaat tcaacgctcg tgctttggct tcgctcatca tcattaccgc agctgcgcca
    42661 tcgtttatgg atgatgcatt acccgctgtc acagaaccga gactatcaaa tgaaggattt
    42721 aaacgggcta aaccttctgc actggcgtca gtgtgtggct gttcatcggt atcaacaacc
    42781 aacgtctgac cgttactttg ggtcattacc gggacgatct catctttaaa tcgtccggcg
    42841 tcaatcgccg ctcgcgcttt ttgttgcgag ctaagtgcgt aagcatcctg caattgacgg
    42901 ctgatgccat attcgcgagc cagattttcg gcggtgacac caatatgata atcattgaag
    42961 gcatcccata acccatcatg cacaagactg tcaaccagct ggctattgcc aagctgtgca
    43021 ccggtgcggc tatcagtcag aacatgtggt gcgcggctca tgttttcctg gccaccggcg
    43081 atgacaatat cagcctcgcc acactgtatc gcctgagtag ccagatgcag tgctttaagc
    43141 ccggaaccgc agacgtcatt aatcgtgatt gcagaaacgc tattaggcag accaccttta
    43201 atagccgatt gccttgccgg attctgccct gcccctgcag tcaacacctg accaagaatt
    43261 acttcatcca ccgcatatgc aggaacgccg gtacgttcta ttaacgcttt cacgaccaga
    43321 ctaccaagtt ccacggcgga atgacccgct aacgcaccgc gaaagcagcc gataggtgtc
    43381 cgtaacgccc cgacaatcac aacgtctttc atcacaacct cgcaccaatt aacagtgcaa
    43441 tagtaaatga ttgttaatta cagttatctt aattgtttaa aaaaagtgat ttttatcaca
    43501 aaggaaatat gcctgagcag tagtcagagt cataactggc acgtaaggtt tgcaaccact
    43561 aacccaccaa tagaggggta gatagggcgt taatctccca tacttaacct ggtttatggt
    43621 aaattgccct ccattttgtt taatttgtag atgatacgtt cagataatgt ctaatatttg
    43681 gtctaaagaa gaaactctgt ggagtttcgc gctctacggc acagccgttg gtgcaggcac
    43741 gctcttcctt cctattcagt taggttcggc aggggctgtg gtcctgttta ttactgctct
    43801 ggtcgcctgg cctttaacat attggccaca taaagcctta tgccagttca tcctctcatc
    43861 gaaaacatca gcaggtgaag ggataacggg cgcagtaaca cactactatg gcaagaagat
    43921 tgataatctg attaccacgc tgtacttcat cgcctttttt gtcgtcgtgt tgatatatgc
    43981 agtggcaatt accaactcac ttacggaaca gctggcaaag catatggtta ttgatcttcg
    44041 catccgtatg ttggtgagtc tgggtgttgt attaatcctg aatctcattt ttctgatggg
    44101 acgccatgca accattcggg taatgggttt tttggtattc ccattgattg cctatttctt
    44161 atttctttcc atttaccttg tcggtagttg gcaacctgat ctattaacga cccaggtaga
    44221 gttcaatcag aatacccttc accagatatg gatatcgatt cccgtgatgg ttttcgcctt
    44281 tagccatacg cccattattt ctacgtttgc catagacaga cgtgaaaaat atggcgaaca
    44341 cgctatggat aaatgcaaaa aaattatgaa agtcgcttat ctcatcatct gcataagtgt
    44401 actgttcttt gtctttagct gcctgctttc tattccacct tcgtatattg aagcggctaa
    44461 agaagaaggg gtcaccattt tatcggcgct ttctatgctg ccgaacgccc cagcatggct
    44521 gtcaatttcc gggattattg tcgcagtagt tgcgatgtcg aaatcattcc tgggtacgta
    44581 ctttggcgtt attgaaggtg ccacggaggt cgtcaaaaca acactacagc aggttggtgt
    44641 aaagaaaagt cgtgcattta accgcgcact atcaattatg ttggtatcgc tgatcacctt
    44701 cattgtttgt tgcattaacc cgaacgcgat ttcgatgatt tacgcgatca gcggcccgct
    44761 cattgccatg atacttttca tcatgcctac gttgtcaacg tatctgatcc cggcgcttaa
    44821 accctggcgt tccatcggaa atctgattac gctgatcgtg ggtatcctgt gcgtatcggt
    44881 aatgttcttt agctgatatc cattgtgaat ggttcaaaat tgctacaatt ttgaaccatt
    44941 ccagttttca taatgttcat tcgttattat tccgcactat cacttccctc atcatatcct
    45001 agtattatca gttagttaaa cacacaaaga gatctgaaca tcttaaataa gactatttaa
    45061 gatgcataac ttagattcgc aagatatctt ctggtcattc aaaaacaatt tgcgatatag
    45121 atattctcat accatgagat agttatctga accaccctca accaggtaag attaaatcag
    45181 tgaaacataa tcatatttaa taacgagaaa tgcattttaa aatgtcttaa tcagaattat
    45241 agcaaataca gattaatcgc aatttttccc agcaaaatta caaaatcatg gtgtattgac
    45301 atcatcaatc accaatgaaa ttatgcaatt atatacggat agggaggttc ttaacatggg
    45361 gctttgtagt cgttataaaa gtcttacatg caatagttgc tcaatgcatt gccaaataat
    45421 gccagaagag tcaccgcgtt tgcagtactg cgcaaactcg tgtttttgta tgtggcccga
    45481 agaaagttca tattttaatc gtggtgtggt agaaggtatc ttaacaaaaa accacaatgc
    45541 gagattaagc ggatatattt ttgtcgattt ttcagtaagt tttttacgtc tattccttga
    45601 aaaagactgg attgactatc ttgcaagtac tgacatgggg attgttttag tcagcgaccg
    45661 caatatgcaa tcgttagcta attactggcg aaaacacaac tcagcaatat ctgctgtcat
    45721 atataatgat gatggtcttg atgttgccaa tgaaaagatc agacaactat ttattggtcg
    45781 ttatttatca tttaccagag gtaacacatt aactcagatg gaattcacca taatggggta
    45841 tatggtttct ggttataatc catatcagat tgctgaagtt ctggatatgg atatccgtag
    45901 catctacgcg tacaagcaac gaatcgaaaa gagaatgggt ggtaaaataa acgaattatt
    45961 tattcgttca cattcggtcc aacattgata ctcaacgacc agccagaatc atactctgtt
    46021 tatacgggca tttattgaaa tgtccgtatt ttataaatag gccgtcccct caagagtaaa
    46081 caccatagta tttaaaacca tcacacgaaa aattcagaag cattacgaaa tatgaattta
    46141 caaacaatag tggcataaat gttaaccatg ttaatttacg taaagtttta cgttgcaaca
    46201 ttaaagcctc atttcaatca tcatgataaa tataaaatta atatatattt atgccgtaaa
    46261 taccaatata cttagcaaac tatgtgatct ccatttcgat tgatttagtg tttattgacg
    46321 tatgtactgg attattaacg ataatatcga gttctggcct gagcaccgaa aattaatatc
    46381 ggtacataac gccgatctta acgtcgttct gacaacgcca gccagtcgat gtttatcact
    46441 tctacttgaa gcttttcctg atgtggttgc acaacaagat tttttcacca gagtctggga
    46501 agaagaaggt atgcgtgtgc ctactaacac gttatatcag aacatatcca ttatcagacg
    46561 cggatttcgc gctgttggtg atactaccca ctcgctaatt gcaaccgtgc cgagaagagg
    46621 attcaagatc cataatgaca ttaacataca aaatcatgta ataaactcgt caacagacgc
    46681 acatacacac aatgccccac ctgccataaa agttaatgcg ggatacaaag agagcattgg
    46741 tggcgcaaag aatttcaata acaaaatcct caaacatata aaaagtcatc taattatgtt
    46801 gagcgcattt gtcataggcg catattctgc atattggcta tggaataata atcaaccgaa
    46861 gccatttttc aaggattaca agactgttgc ggaaattaat ggctgccatt ttaatgtaac
    46921 agaagataca attgacgggt tgaaagagtt cgataaatat aaaacacgga tactggattc
    46981 ggggattaac tgtaaaaaac atccgtggtt atacttccct cttgcaaaat cctcaccggg
    47041 gatgattgtt atggcatgca ataaaaatta taaccaacat gaagtggcag actgtttaac
    47101 cctgtcttac cgtgaggtta atcgtgattg attataaaaa aaacttatta
//
LOCUS       DAZGSP010000031        45857 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZGSP010000031 DAZGSP010000000
VERSION     DAZGSP010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.031"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            232..858
                     /locus_tag="ACHK74_001879"
     CDS             232..858
                     /locus_tag="ACHK74_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFP0220710.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            879..2981
                     /locus_tag="ACHK74_001880"
     CDS             879..2981
                     /locus_tag="ACHK74_001880"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFP0220711.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            2994..3632
                     /locus_tag="ACHK74_001881"
     CDS             2994..3632
                     /locus_tag="ACHK74_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFP0220712.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3696..4364
                     /locus_tag="ACHK74_001882"
     CDS             3696..4364
                     /locus_tag="ACHK74_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP0220713.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4361..5146
                     /gene="phsC"
                     /locus_tag="ACHK74_001883"
     CDS             4361..5146
                     /gene="phsC"
                     /locus_tag="ACHK74_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFP0220714.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYCIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5150..5962
                     /gene="ydhT"
                     /locus_tag="ACHK74_001884"
     CDS             5150..5962
                     /gene="ydhT"
                     /locus_tag="ACHK74_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFP0220715.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVASTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLDEQHQFVEC"
     gene            complement(5974..7578)
                     /locus_tag="ACHK74_001885"
     CDS             complement(5974..7578)
                     /locus_tag="ACHK74_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFP0220716.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7704..8009)
                     /locus_tag="ACHK74_001886"
     CDS             complement(7704..8009)
                     /locus_tag="ACHK74_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFP0220717.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8117..8193)
                     /locus_tag="ACHK74_001887"
     tRNA            complement(8117..8193)
                     /locus_tag="ACHK74_001887"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8157..8159),aa:Val,seq:gac)
     gene            complement(8198..8274)
                     /locus_tag="ACHK74_001888"
     tRNA            complement(8198..8274)
                     /locus_tag="ACHK74_001888"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8238..8240),aa:Val,seq:gac)
     gene            8582..9838
                     /locus_tag="ACHK74_001889"
     CDS             8582..9838
                     /locus_tag="ACHK74_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFP0220718.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9879..11252)
                     /gene="mdtK"
                     /locus_tag="ACHK74_001890"
     CDS             complement(9879..11252)
                     /gene="mdtK"
                     /locus_tag="ACHK74_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFP0220719.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVKPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            11467..12108
                     /gene="ribE"
                     /locus_tag="ACHK74_001891"
     CDS             11467..12108
                     /gene="ribE"
                     /locus_tag="ACHK74_001891"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFP0220720.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQLGTEA"
     gene            complement(12148..13296)
                     /gene="cfa"
                     /locus_tag="ACHK74_001892"
     CDS             complement(12148..13296)
                     /gene="cfa"
                     /locus_tag="ACHK74_001892"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFP0220721.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13587..14798)
                     /gene="punC"
                     /locus_tag="ACHK74_001893"
     CDS             complement(13587..14798)
                     /gene="punC"
                     /locus_tag="ACHK74_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFP0220722.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14911..15843
                     /gene="punR"
                     /locus_tag="ACHK74_001894"
     CDS             14911..15843
                     /gene="punR"
                     /locus_tag="ACHK74_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFP0220723.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15840..16865)
                     /gene="purR"
                     /locus_tag="ACHK74_001895"
     CDS             complement(15840..16865)
                     /gene="purR"
                     /locus_tag="ACHK74_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFP0220724.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17164..17253
                     /gene="cydH"
                     /locus_tag="ACHK74_001896"
                     /gene_synonym="ynhF"
     CDS             17164..17253
                     /gene="cydH"
                     /locus_tag="ACHK74_001896"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFP0220725.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17419..18588
                     /locus_tag="ACHK74_001897"
     CDS             17419..18588
                     /locus_tag="ACHK74_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0220726.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18734..19315)
                     /gene="sodB"
                     /locus_tag="ACHK74_001898"
     CDS             complement(18734..19315)
                     /gene="sodB"
                     /locus_tag="ACHK74_001898"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFP0220727.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19443..20258)
                     /gene="mepH"
                     /locus_tag="ACHK74_001899"
     CDS             complement(19443..20258)
                     /gene="mepH"
                     /locus_tag="ACHK74_001899"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFP0220728.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20593..20940
                     /gene="grxD"
                     /locus_tag="ACHK74_001900"
     CDS             20593..20940
                     /gene="grxD"
                     /locus_tag="ACHK74_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFP0220729.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20991..25607)
                     /locus_tag="ACHK74_001901"
     CDS             complement(20991..25607)
                     /locus_tag="ACHK74_001901"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFP0220730.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASSTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25700..26347)
                     /gene="rnt"
                     /locus_tag="ACHK74_001902"
     CDS             complement(25700..26347)
                     /gene="rnt"
                     /locus_tag="ACHK74_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFP0220731.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26450..26857)
                     /gene="gloA"
                     /locus_tag="ACHK74_001903"
     CDS             complement(26450..26857)
                     /gene="gloA"
                     /locus_tag="ACHK74_001903"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFP0220732.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26938..28035)
                     /gene="nemA"
                     /locus_tag="ACHK74_001904"
     CDS             complement(26938..28035)
                     /gene="nemA"
                     /locus_tag="ACHK74_001904"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFP0220733.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28072..28671)
                     /gene="nemR"
                     /locus_tag="ACHK74_001905"
     CDS             complement(28072..28671)
                     /gene="nemR"
                     /locus_tag="ACHK74_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFP0220734.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            28774..29013
                     /locus_tag="ACHK74_001906"
     CDS             28774..29013
                     /locus_tag="ACHK74_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFP0220735.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPKQPSLF"
     gene            29062..29958
                     /locus_tag="ACHK74_001907"
     CDS             29062..29958
                     /locus_tag="ACHK74_001907"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFP0220736.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            30039..30560
                     /gene="sodC"
                     /locus_tag="ACHK74_001908"
     CDS             30039..30560
                     /gene="sodC"
                     /locus_tag="ACHK74_001908"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFP0220737.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30561..32573)
                     /locus_tag="ACHK74_001909"
     CDS             complement(30561..32573)
                     /locus_tag="ACHK74_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP0220738.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32573..33430)
                     /locus_tag="ACHK74_001910"
     CDS             complement(32573..33430)
                     /locus_tag="ACHK74_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFP0220739.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33433..33669)
                     /locus_tag="ACHK74_001911"
     CDS             complement(33433..33669)
                     /locus_tag="ACHK74_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFP0220740.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33870..34304
                     /gene="slyA"
                     /locus_tag="ACHK74_001912"
     CDS             33870..34304
                     /gene="slyA"
                     /locus_tag="ACHK74_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFP0220741.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34351..34818)
                     /gene="slyB"
                     /locus_tag="ACHK74_001913"
     CDS             complement(34351..34818)
                     /gene="slyB"
                     /locus_tag="ACHK74_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFP0220742.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35092..36201
                     /gene="anmK"
                     /locus_tag="ACHK74_001914"
     CDS             35092..36201
                     /gene="anmK"
                     /locus_tag="ACHK74_001914"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFP0220743.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWAQAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36299..36628
                     /gene="mliC"
                     /locus_tag="ACHK74_001915"
     CDS             36299..36628
                     /gene="mliC"
                     /locus_tag="ACHK74_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFP0220744.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36687..37343
                     /gene="pdxH"
                     /locus_tag="ACHK74_001916"
     CDS             36687..37343
                     /gene="pdxH"
                     /locus_tag="ACHK74_001916"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFP0220745.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37472..38746
                     /gene="tyrS"
                     /locus_tag="ACHK74_001917"
     CDS             37472..38746
                     /gene="tyrS"
                     /locus_tag="ACHK74_001917"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFP0220746.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38805..39668
                     /gene="pdxY"
                     /locus_tag="ACHK74_001918"
     CDS             38805..39668
                     /gene="pdxY"
                     /locus_tag="ACHK74_001918"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFP0220747.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39712..40317)
                     /gene="gstA"
                     /locus_tag="ACHK74_001919"
     CDS             complement(39712..40317)
                     /gene="gstA"
                     /locus_tag="ACHK74_001919"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFP0220748.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40423..41925)
                     /gene="dtpA"
                     /locus_tag="ACHK74_001920"
     CDS             complement(40423..41925)
                     /gene="dtpA"
                     /locus_tag="ACHK74_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFP0220749.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42536..43171)
                     /gene="nth"
                     /locus_tag="ACHK74_001921"
     CDS             complement(42536..43171)
                     /gene="nth"
                     /locus_tag="ACHK74_001921"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFP0220750.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQYNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43171..43866)
                     /gene="rsxE"
                     /locus_tag="ACHK74_001922"
     CDS             complement(43171..43866)
                     /gene="rsxE"
                     /locus_tag="ACHK74_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFP0220751.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(43870..44490)
                     /gene="rsxG"
                     /locus_tag="ACHK74_001923"
     CDS             complement(43870..44490)
                     /gene="rsxG"
                     /locus_tag="ACHK74_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFP0220752.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAQELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(44494..45552)
                     /gene="rsxD"
                     /locus_tag="ACHK74_001924"
     CDS             complement(44494..45552)
                     /gene="rsxD"
                     /locus_tag="ACHK74_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFP0220753.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45553..>45857)
                     /locus_tag="ACHK74_001925"
     CDS             complement(45553..>45857)
                     /locus_tag="ACHK74_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220754.1"
                     /translation="NAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRK
                     AAVEAAIARAKVRKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT        11537 a        11341 c        11965 g        11014 t
ORIGIN      
        1 tgctttgtaa ttttaattga taacgatcaa tgttaagggc tcagaataat cacattattt
       61 tttctcctaa ccataaagga ttagtttatc ggcgttaaat cttttctcat acactatcgg
      121 gttagggaaa atatctggtg ttggggacag agtcagcagc gtattagttg aatgcttaat
      181 atcaggctgc tgaattgtca gcactgtgtt tgcgaagagg atgcgaaata aatgaacccg
      241 gttgatcgtc cactattaga tattggctta acgcgattag aattcttgcg tatatcagga
      301 aaaggccttg ctggtctaac aattgcccct gcgttactgt cacttttggg ttgcaaacag
      361 gaagatattg atagcggcac agtagggttg ataaatacac ccaaaggggt gctggtgacc
      421 cagcgagcgc gatgcacggg ttgccaccgc tgtgaaatct cttgtaccaa cttcaacgat
      481 ggctcagtag ggacattctt ctcccgtatc aaaatccacc gcaattattt ctttggcgac
      541 aacggggttg gctctggcgg cggcctgtat ggcgatctca actataccgc ggacacctgc
      601 cgtcaatgca aagagccgca atgcatgaac gtctgcccga ttggtgcgat tacctggcag
      661 cagaaagaag gctgtattac cgtcgatcat aaacgctgta ttggctgtag cgcctgtact
      721 acggcatgcc cgtggatgat ggccaccgta aataccgaaa gtaaaaaatc ctcgaaatgt
      781 gtgttatgcg gtgaatgcgc aaacgcctgc ccaacagggg cgttaaaaat tatcgagtgg
      841 aaagatatta ctgtgtgaat cttgcaaagg aaaaaatcat ggctaacggt tggacaggta
      901 atatattaag agtcaatctc acgacaggaa atattaccct cgaagattcc agtaagttta
      961 aaagttttgt cggtggcatg ggcttcggct acaaaattat gtatgacgaa gtaccgccag
     1021 gcacgaaacc tttcgatgaa gcgaataaat tagtctttgc taccggccca ttaactggat
     1081 ctggtgcccc ctgtagttct cgcgtaaata tcacctcact ttctactttt accaaaggaa
     1141 atttagttgt cgatgcccat atgggtggct tttttgcagc gcaaatgaaa ttcgctggat
     1201 acgacgtcat tattatcgaa gggaaggcga aatcaccggt atggctgaag attaaagatg
     1261 acaaagttag cctggaaaaa gccgatttct tatggggaaa agggacgcgc gcaacgacgg
     1321 aagaaatttg tcgattgacc agtccggaaa cctgtgtggc ggctattggt caggctgggg
     1381 aaaaccttgt tcctctctct ggcatgttga atagccgtaa ccacagcggc ggtgcgggaa
     1441 ctggcgcaat tatgggttcg aaaaacctga aagcgattgc ggttgaaggg acgaaagggg
     1501 tcaacattgc cgatcgtcag gagatgaagc gtctcaacga ttacatgatg actgaactta
     1561 ttggtgcgaa taacaaccat gtcgtgccaa gtacgccgca atcgtgggca gagtattcag
     1621 atcccaagtc acgttggaca gcacgtaaag ggctgttttg gggcgcggct gaaggtggtc
     1681 cgattgaaac gggggaaatt ccgccaggca atcagaacac ggtcggcttt cgtacctata
     1741 aatccgtttt tgacttaggt ccggcggcag agaaatacac agtaaaaatg agtggctgcc
     1801 actcttgccc gatccgttgt atgacccaaa tgaatattcc tcgggtgaaa gagtttggcg
     1861 tgcccagcac aggtggtaac acttgtgtag ccaactttgt ccataccacc atcttcccga
     1921 acgggccgaa agattttgaa gataaagacg atggtcgtgt gattggtaac ctggttggtc
     1981 tgaatttgtt cgatgactac ggcctatggt gtaactacgg gcagctgcat cgcgacttta
     2041 cttattgtta cagcaaaggg gtgttcaagc gtgttctgcc agctgaagag tatgcggaaa
     2101 ttcactggga tcaactggaa gcgggtgacg ttaatttcat taaagatttt tactaccgtc
     2161 tggcgcatcg tgtgggtgag ctgagtcacc tggctgatgg ttcatatgcc atcgcagaac
     2221 gctggaattt gggtgaagag tactggggct acgcaaaaaa taaactctgg tcgccgtttg
     2281 gctatccggt tcaccatgcc aatgaagcgt cagcgcaggt cggttccatt gttaactgta
     2341 tgttcaaccg tgactgcatg acgcataccc atatcaattt tattggttcg ggtttgccat
     2401 tgaaactgca gcgtgaagtg gcgaaagagc tttttggttc tgaagatgcc tacgatgaaa
     2461 ccaaaaacta cacgccaatc aatgacgcaa aaattaaata tgcaaagtgg tcgctgttgc
     2521 gggtctgttt gcataacgcc gtcaccctgt gcaactgggt ctggccaatg accgtttcgc
     2581 cgctgaaaag ccgtaattac cggggcgatc tggcgcttga agccaaattc ttcaaagcga
     2641 tcaccggcga agagatgact caggaaaaat tagatttagc tgcagagcgt atctttacgt
     2701 tgcatcgtgc ctacacggta aaactgatgc aaaccaaaga tatgcgtaat gaacacgatc
     2761 ttatctgttc ctgggtattc gacaaggatc cgcagatccc ggtctttact gaaggtactg
     2821 acaaaatgga tcgtgacgat atgcatgctt cgctcacgat gttctacaaa gaaatgggct
     2881 gggacccaca gcttggttgt ccgacccgcg aaacattgca gcgtctgggg ctggaagata
     2941 ttgccgccga tctggcagca cacaatctac tgcctgtgta aggaatgggt gagatgaacc
     3001 atcgggatga attgccgtta gcgaaagtca gcgaagttga cgaggcgaag cggcaatggt
     3061 tacaggggat gcgtcacccc gttgatacgg taaccgaacc agaaccggct gaaatcctgg
     3121 cagagttcat tcgccagcat tctgcggctg ggcagctggt ggcgcgcgcc gtgtttctct
     3181 cgccacccta ttcggttgca gaagaggagc tgtcagtttt gctggaaagc attaagcaga
     3241 acggagacta cgcggatatc gcttgtatga cgggaagcca ggatgattac tattactcaa
     3301 cgcaggccat gagtgaaaac tacgcggcga tgtctttgca agttgtggag caggacattt
     3361 gtcgcgccat tgcacacgcc gttcgttttg agtgccagac atatcctcgt ccttacaaag
     3421 tcgccatgct gatgcaagcg ccatattact tccaggaagc gcagattgaa gccgcgatcg
     3481 ccgcgatgga tgttgcacct gaatatgccg atatacggca ggtggagtca tcgacggcgg
     3541 tgttgtactt gtttagcgag cgatttatga cctatggcaa ggcttatggc ttatgcgaat
     3601 ggttcgaggt agagcagttt cagaacccct gaaatttcag ggagatgtca tggatagggt
     3661 ggacagttgc agccactgca ttaggggata atcagatgtc cttcactcga cgcaaatttg
     3721 ttctggggat gggaacagta atctttttta ctggttctgc gtcttctctg ttagcgaaca
     3781 cgaggcaaga aaaggaagtt cggtatgcca tgattcatga cgagtcacgg tgcaatggct
     3841 gtaatatttg cgcccgcgca tgccgtaaaa ctaaccacgt ccccgctcag ggaagccgtt
     3901 tatcgatagc acacattcca gtcaccgata acgacaacga aacgcagtat cacttttttc
     3961 gccagtcatg tcagcactgc gaagatgctc cgtgcatcga cgtatgcccg acaggggcgt
     4021 cgtggcgtga tgagcagggg atcgtgcggg tagaaaaatc gcagtgcatc ggctgcagtt
     4081 attgcattgg cgcttgtccg taccaggttc gctaccttaa ccctgtcact aaagtggcag
     4141 ataaatgtga tttctgcgct gagtcccgac tggcaaaagg ctttccgccc atttgcgtca
     4201 gcgcctgccc ggaacacgca ttgatctttg gtcgcgaaga tagcccagaa attcaggcgt
     4261 ggttgcagca aaataaatac tatcaatatc agctacctgg tgccgggaaa ccgcatctgt
     4321 atcgccggtt cggtcaacat ttgattaaaa aggaaaatgt atgaacccgt cgcaacatgc
     4381 tgaacagttt cagagccagt tagcgaacta tgtgccacag ttcactcccg aattttggcc
     4441 ggtgtggttg atcattgccg gagtattgct ggttgggatg tggttggtgc tggggctgca
     4501 tgccttgctt cgtgctcgtg gcgtgaagaa atcagtcacc gattatggtg agaagattta
     4561 tctttactgc aaagcggtca gattatggca ctggtcgaat gcgttactct ttgtattgtt
     4621 gctggcaagt gggctgataa atcactttgc gctggtgggc gcaactgcgg ttaaaagtct
     4681 ggttgcggtg catgaagttt gcggattttt gttactcgca tgctggctcg gctttgtgct
     4741 gatcaatgcc gttgggggta atggtcacca ctattgcatt cgtcgtcagg ggtggctgga
     4801 gcgagcggca aaacaaacgc gattttattt gtttggcatt atgcaggggg aagaacatcc
     4861 tttcccggca acaacccagt ctaaatttaa tcccttacag caggtcgcct atgttggtgt
     4921 tatgtatgga ttgctgccgt tgttactatt gacggggctg ctgtgtctct atccgcaagc
     4981 cgtgggagat gtgtttcctg gcgtaagata ctggttattg caggcgcatt ttgctctggc
     5041 atttataagc ctctttttta tcttcggtca tctttatctt tgcaccacgg ggcgtacgcc
     5101 acacgaaacc tttaaaagca tggtcgatgg ctatcaccgg cactaacgaa tgatcattac
     5161 acgagcagac ctgcgagaat ggcgtatcgg agcggtgatg tatcgctggt tcctgcgcca
     5221 tttcccacgc ggcggtagtt atgccgatat tcatcatgca ctgattgagg aaggatatac
     5281 ggactgggcg gagagtctgg ttgagtatgc gtggaaaaaa tggctggcgg atgaaaactt
     5341 cgcgcatcag gaagtttcct ctatgcaaaa actggccaca gaccccggag agataccatt
     5401 ttgcagtcag tttgcccgca gcgacgatca tgctcgcatc ggctgttgtg aggataacgc
     5461 ccgaatcgca accgctgggt atgccgcgca aattgccagt atgggttata gcgtgcggat
     5521 tggaagtgtc ggctttaaca gtcacattgg cagctccggt gaacgcgcac gggttgctgt
     5581 aaccggtaat tcttcgcgga taagcagcgc tggagatagt agtcggattg cgaataccgg
     5641 aatgcgagta cgcgtttgta cattgggtga acggtgtcat gtcgccagta atggtgacct
     5701 ggtgcaaatc gccagctttg gcgcaaacgc cagaattgcc aacagcggcg acaatgtaca
     5761 catcattgcc agcggtgaaa attcaacggt agcgagcacg ggcgtggtcg actcaattat
     5821 tctcgggcca gggggaagcg ccgcactggc atatcatgac ggcgaaagag ttcgcttcgc
     5881 cgtcgccatt gaaggtgaaa acaatattcg aaccggagtc cgctatcgtc ttgatgaaca
     5941 gcatcagttt gttgagtgct gatagcttta cctttaatca aacgaaagac gccgacgagc
     6001 acgggatgca ggctttacga ccgcccgagc catcgcctca ccaatttctg cacatgccgt
     6061 aagtccctga acgaaaggct ggtcgtgcat caaccagggc aacgcgccga acgctacgcg
     6121 tccacgaata tcttcgggtt gctgtaacgt ataatcttcg ccaacatcag ggatttcatc
     6181 ccctgttttc tgtaattgct cgcgtagccc agggaaagga atatctttca ctttaagcgg
     6241 acgctgtccg cgggcatcaa taaaaacgtc aaacgagtag ctgttgtctt ccgttttcag
     6301 gacggtgcgc gactcattaa tttccatttt gtagtcttca ccgagggcga gaatatgaat
     6361 gattcccgct tcacgtaagg caagtaggcg acgaatagac tctgaaggga ttgccgcata
     6421 attatcgatg aaaaccctgg caaggccttt actgaaccgt ttatggtcgt gttcattcag
     6481 atgaggaaca atttcctgta cggcttcatg caggcgcaga atgacataac gccagggaac
     6541 agtatgtttt tctcgtttat tgcgttcgac ttcctggaga tttttttctg cccagtcaaa
     6601 tgggtcgcgt tgtttgcgct cggcaaacca ggcttgagca aaggaatcga cattcaggct
     6661 ctctaaggct atgcgttggc tccagtctgg atcagcaaac ttgatttcct ctactatcaa
     6721 tctaaatacc cgatccaaaa ggccctcttc acctttttga atctcagcat ttaatgcctg
     6781 atcggtgacg atgtgtaagg gctcgtaggg aatagggcaa tagaaatcgg cttcgggtaa
     6841 aatacccgtg cgcgacatca acgtaatatt tagcttttca cttgcgttat cgcggttaaa
     6901 aacgacgtgt tgtttatcat cttcaatgaa cgaaccatgc tgaatagcca ctgccattgc
     6961 cgcatccagt ccgctcaagg atgttcccat aatacccacg ttacacgcat cgacctttgc
     7021 ttccatcagg cctgaccacg gactgggaaa atacgttctg gttgcttctt cttcatcagg
     7081 ccagacgtga cccgtggcaa tcaccgctaa atcaaacgtc tcgctgggta aatcctgatt
     7141 tgtagcgagc atgacgccag catttgtaat ttgcagatcg gtaacctggc atgattcata
     7201 aacagccact gcaaattttt gctgtcgtgc ctggtctact aatcgtaaaa attgatcgcg
     7261 gaaatattcg cccagcagaa ttcgcggtaa aaactgacga tcgtgcaagg tttctttttt
     7321 aacgccataa cgctggagat ggctggcttc ttgcttttgt agccattcga gatacgtaca
     7381 attaatcggc ggtatttcaa tactggcaat atttgccagc atcatttttg agttttcctc
     7441 atcactgtat ggcatcccga caccggcctc gtcagcctgc tcgaagatag aaatagaaag
     7501 tggagtttgt tgctgtagaa gcgagaataa ggtgtagatc cccgtaggcc cggcacccac
     7561 aatagcaatt tttttcattg ttacttacct gtacgcattc tttcgaattt agtgttatca
     7621 gataagtttg gttgagattt ttagaatttg agaaatttca gacagggatg aaaagggcgc
     7681 taaccgccct tgattaaatt ctgtcaggcg agttttgcct gattgatttg actcagtggc
     7741 tcattgacat cgaaaacttt ggcgacaact tcctcaacgc cgagattttt aagtcgggca
     7801 gtatgttttt ccagataggc aagggcgctt ttttcatcag taaaaagata gatcccaccg
     7861 gcttcgtggt tcttttcact ttctgtccat accttccaca gaaaaccagg ttcctgatta
     7921 atcgactcag caagcggttt aagctgctca gccattgcgt cgccaaatgg gccattaaaa
     7981 gcaaaatgaa gttgtaacaa agttgccata gcgattctcc ttctgataac gcatacgaag
     8041 acagtgtaaa tcttttaacg tattggatac agagaagaaa cagagggaaa aaggagaaga
     8101 ccagattaag aaaatctggt gcgtccgagt ggactcgaac caccgacccc caccatgtca
     8161 aggtggtgct ctaaccaact gagctacgga cgcaggatgg tgcgttcaat tggactcgaa
     8221 ccaacgaccc ccaccatgtc aaggtggtgc tctaaccaac tgagctatga acgcaacgtt
     8281 gtaggtgaca acggggacga atattagcgg cagagtggga aggtggcaag aggcaaaacg
     8341 taattttctg cgctatttcg accgtttgca gagcttttaa gcaaattggc tatattttgt
     8401 tgatttgcaa gggcgatttt tattcaggat cgcatttaca tctgatacaa cccaataatt
     8461 cgtgtatcag agtttaaaca gagttgtttg ccaccatctc aaatctgtcg tggataaatc
     8521 ctccgtagcc tttacactta ggtagcaata aaggcacaca gatgcagcaa aggaataaat
     8581 catgggatct gatgcgaaaa acttgatgag cgacgggaat gtgcaaattg ttaagaccgg
     8641 cgaggtcatt ggcgcgacgc aacttactga aggcgagttg attgttgaag ctggcggaag
     8701 agccgaaaat accgtggtca cgggggctgg ctggttgaaa gtggcaaccg gtgggatcgc
     8761 caaatgcaca cagtacggta acaatggcac gctatcggtc agcgacggtg ccattgccac
     8821 agatattgtt cagtccgagg gaggcgcaat tagtctctct acgctcgcta cggttaatgg
     8881 ccgccatccc gaaggtgaat tcagcgttga tcagggttat gcctgcggtt tgttgctgga
     8941 aaatggcggt aacctgcgtg tactggaagg acatcgcgcg gaaaaaatca ttctcgatca
     9001 agagggcggc ctgttggtta atgggacaac ctcagcggtc gtggtagatg aaggtggtga
     9061 attgttggtg tatccaggtg gggaagccag caattgtgag attaatcagg gcggcgtttt
     9121 tatgctggcc gggaaagcca gtgatacgtt gcttgctggt ggcaccatga ataatctcgg
     9181 tggtgaagac tctgacacta ttgttgagaa tggatccatt tatcgtctgg ggacggatgg
     9241 ccttcagctc tacagttccg gtaagacgca aaacctgtcc gtgaatgtgg gtggtcgggc
     9301 tgaagtgcat gccggtacgc tggaaaatgc ggtaatacaa ggtggaacag tgatcctgtt
     9361 gtcacccacc agcgcggacg aaaattttgt cgtagaggaa gatcgcgcac cggttgaact
     9421 gaccgggagt gttgcattac tggacggcgc ttcaatgatt attggctatg gcgcagatct
     9481 gcaacaatca acgattactg tacagcaggg cggtgtgttg attctcgacg gcagtacggt
     9541 aaaaggtgac agtgtcactt ttagtgttgg taacatcaat ctgaacggcg gaaaactgtg
     9601 gctgatcact ggtgcggcaa cgcatgtgca actgaaagtg aaacgcctgc gcggagaggg
     9661 agcgatttgc ctgcaaacca gtgcgaaaga aatctcacct gacttcatca atgtgaaagg
     9721 ggaagttacc ggggatatac acgttgagat aacagatgcc agtcggcaaa ctctgtgcaa
     9781 cgctctgaaa ttacagccag acgaagacgg gattggcgca acgctccagc ctgcgtaaaa
     9841 acacaggcgc aacccgtgaa ggttgcgcct tgtctttatt agcgggatgc tcgttgcaga
     9901 atgataactg acggcagacg ttgcaggaac cgcatacgca gcatcatcat aatggctgcc
     9961 gacgtcaggc caataataaa gcctatccag aagcctgctg gccccatagg tttaacgacc
    10021 agatcggtca gtgccagaat atagccgctt ggcaagccca gcacccagta agccgtaaag
    10081 gtgatataga aaatggaacg cgtatcttta taaccacgca aaatcccact gccaatcacc
    10141 tggattgagt cagaaatctg atataccgcc gccagcaaca tcaaatgcgc agccagcgtt
    10201 acaacctcgg gattgtcgtt gtacaacagg gcgatttgct cccgcagtga aaccgtgaat
    10261 atggccgtca gggttgccat acagacaccc accataagcc cggtccgcgc agcggtttgc
    10321 gcatccagcg ttgagccctg acccagacga taacctacgc ggatagttac cgctgccgcc
    10381 agcgacattg gaagcacaaa catcagtgaa ctaaagttca gggcaatctg gtgtcctgcg
    10441 acatcaacaa taccgagcgg agacactaac agagccacga cggcaaacag tgtcacttca
    10501 aagaatagtg ccagcgcaat cggcaaaccg agttgaatca gtcgtttcat aaccgcagga
    10561 tcgggtttcg cggtgccttt ttcgttacga atatcgcgca tggagcgggc gcgtttaatg
    10621 taagaaacca tggcaaggaa catgacccaa tacaccgccg cagtagccac gccacaacca
    10681 acgccaccga gctcaggcat accgaaatga ccataaataa agatatagtt caccggaatg
    10741 ttcaccagca ggccgataaa gcccattacc atacccggct tggtttttgc cagaccttca
    10801 cattggttac gggcaacctg gaagaacaga tagcccggcg cgccccacaa caacgcacgc
    10861 agataaccca cggctttgtc cgccagagcc ggatcgatgt tttccatgga gcggataatg
    10921 taacctgcat tccacagcac aagcataatg agaacggaaa caaaacctgc cagccagaag
    10981 ccctgtcgca cctgatgcgc aatgcgctca cgtcgaccgg aaccatttaa ttgcgcgata
    11041 accggcgtta atgccagcag cagtccgtga ccaaagagga tcgccggaag ccagatagaa
    11101 gtaccgatag cgaccgccgc catgtcggtg gcactatagc cgcccgccat cacggtatcg
    11161 acaaaaccca tcgcagtttg ggcgatttgc gcgagaatca ccgggattgc taatgctaat
    11221 aacagacgcg cttcactgat atacttctgc acgtgaacac cttttatttg tagttatatg
    11281 agagactaaa aagccgcctg aatgggcagc aaaaagaaga agcaggggaa attcagtcta
    11341 ttgtagcgag gatttattaa ttctccagtg aaaaaatagt cattcttacc ccttcactgg
    11401 taagggcgtt tgccacctgc tattgtgctg aacagaatgt cttaactgat ttcaggagtt
    11461 gtaagtatgt ttacggggat tgtacagggc accgcaaaac tggtgtcgat tgacgagaaa
    11521 ccaaattttc gtacgcatgt ggtggagtta cccgaccaca tgctggacgg cctggaaaca
    11581 ggtgcttccg tggcgcataa cggttgctgc ctgaccgtga cggaaattaa cggcaaccat
    11641 gtcagttttg acctgatgaa agaaacgtta cgcattacca atcttggcga tttaaaagtg
    11701 ggggattggg taaacgttga gcgtgcggcg aaattcagtg acgaaattgg cggacactta
    11761 atgtcaggtc atattatgac caccgctgaa gtggcgaaaa tattaacctc ggaaaataat
    11821 cgccagatct ggtttaaagt ccaggatagt cagttgatga aatatatcct gtacaaagga
    11881 tttattggca tcgacggtat tagcctgacc gtcggcgaag tcacgccaac gcgtttttgc
    11941 gtccatttaa ttccggaaac tctggaacgc acgactcttg ggaagaaaaa actcggcgca
    12001 cgcgtcaaca ttgaaattga tccacaaact caggcagtgg tagatacggt agaacgtgtg
    12061 ctggcggcac gagaaaatgc catgaatcaa ctaggtacag aagcctgatc tgaaatcgcc
    12121 cccggagagg gggcgataga atagccttta gcgagcgact cgaaggccgt tttcaacacc
    12181 gcgtgagaac acgacctgcc agagctgaat atcacgggcg cggaaagcac ctgcacaggc
    12241 attcagataa taggtaaaca ttcgtttaaa gcgttcactg tagttatccg caatttctgg
    12301 ccatgcggcg aggaatcgtt cataccacgc catcaacgta gtatcgtaat cagcaccgaa
    12361 gttatgccag tcttccatca cgaagtgggg ttcgctggat tgagcaatct ggcgtacaga
    12421 gggcaggcaa ccgttcggaa aaatatattt attaatccag ggatcaacat tcagatcggt
    12481 ttttttcgaa ccgatagtat ggagcaggaa tataccttcc ggtttcaaat tacgatccac
    12541 caccgcaaaa taggtatcgt aatttttcgg tccgacgtgc tcgaacatcc ccacagaaac
    12601 aatacgatca aactggtcgt tcaggtcacg ataatcttgc agcaaaatgg tgacatccag
    12661 gccttcacag cgttcctgag ccattttttg ctgttcggca gaaatggtga cgccgaccac
    12721 gcttacgtca tagttagatg ccatgtagtg cgccagtccg ccccagccgc agccaatatc
    12781 cagtacgcgc atccctggtt ttaactgcaa tttttcacaa atcattttga gcttcgcctg
    12841 ctgggcagat tccagattat cggcatcttt ccagtaagcg caggaatatt gcatgaaggg
    12901 atcaagcatg cggctgaaca agtcattacc caaatcgtaa tgctccttgc cgactatcca
    12961 ggcacgtttt ttactctgca gattgaagag acgagcgctg gcaatacgca gcgtgtcttt
    13021 gaaatgatgg gggagttggt tctcgagacc tgcgcgtaag actttgctaa aaaacatatc
    13081 cagtcggtca cattcccacc agccatccat ataactttcg cctaacccca aagagccttc
    13141 ttgcagaacg cgtttaaaaa aatcggggtt tttcacacga atatccgccg gggcagaacc
    13201 gttaatggct ataccggcac ggctaagtaa ttcgttggcg atacggtacc agttgtcatc
    13261 cggtacactg acttcttcta tacacgatga actcatagtt tctccatcac tggtcggtga
    13321 tcagaacctt aaaacagcgt agacgctttt tgggctttgt gagaaatctc gcagaaaaaa
    13381 ccgcgagtct ataatccgac gtagaacaga gggaaaggag gaacctttgc cgaaatgacc
    13441 atccatagtg agtcgggaac gatcctgtcc ccgtaaaaac ataatattta tcgtagcttt
    13501 aacaactcga aattagtata ggccgctaat ttcaatcata caacaaaatt ttgttagcag
    13561 cctaagtata agtatatcga tatagatcag tgtgattcgc tatgagcgac ttcggcattg
    13621 ccatgattct ggcagccaac ttcttcacaa cgttgcatca tgtaacccag agcgaccagc
    13681 actactgttg ataacatcac gctggtggtg gtgagcaatg gcgtgctgat actgatcagc
    13741 caggaaacta ccagacttgc gaggaagcac agacccagtt gtagagtgtt ctgcaacgct
    13801 gcggcgcgac cagttgcgtg tgggaaggga cgcagcgcct gggcgacaac aatcgggtag
    13861 atcgcgccat tggcaatcgc catcacacag aatgggatca ggatttcgac cagcgacaca
    13921 tggctaatga agcctgcagc ccaggtcgca atgacgctga ctgcaaacag caccagcaac
    13981 cacggtaata actgcttgcc ttgccatttc tgcagtgcgg cgcgacagcc ataaccacca
    14041 atcagaaacg cgatagtttg cgggacataa cttaaaccaa taactgccgg gctgtagccc
    14101 atttcactaa ggatgaacgg tgaaccggtc agccatgcaa aaaaactggc tgaacaggct
    14161 gcatatatca gcacgttgcc gcgataggtt ttagaacgta gcaggtcggt aaaggtcaga
    14221 ccatcctgac tattgttacg ggctttcgtc gtgggtttga gccagaaaat aggcagaatc
    14281 agcaccacgg taatggcaaa cagggtggcg aaaatcgcct gccaggaaaa atggaccagc
    14341 agccagcttc ctaacagggg agccagtgcc ggagacagac ccaccagcgg catgatggtc
    14401 gcaaaaatac ggttgacttt ctgtgaagga taataatctg tcactaatgc ttgccagata
    14461 accgccgcgg cgcagacccc cacagcctgt acaaaacgca ataccagcag cgtagcggcg
    14521 ttttctaccc acagcatccc cagactacct aacgcaaaaa ttgtcaggcc gattaataac
    14581 accggtttac gaccataacg gtcggagagt ggcccccaca gaagctgggc tgcggcaaaa
    14641 ccggcaagga acagactaag gctggcactg acagcagacg caggcgtttg caggtcggcc
    14701 tgtatggcgg cgaaagcagg cagatacata tcggttgcca gaaaaccgag tacgctcaaa
    14761 cccgccagcc agactaaaaa tcttttccca ggttgcatgt atattctcta aatctctcaa
    14821 ttaaacaccg ccgcaaagtg tagggagtga aattagtgtt gtgaaacggt aatatttgat
    14881 gactggtttc aaaaattttg caggcagact atgtggtcag aatattcact cgaagttgtt
    14941 gatgcggtag cgcgtaatgg tagttttagc gctgcggcac aggagctgca tcgcgttcct
    15001 tctgcggtca gctataccgt gcgtcagctg gaagagtggc tggcggtacc gctctttgaa
    15061 cgacggcacc gtgatgtgga gctgaccgct gctggagcgt ggtttctcaa agaagggcgc
    15121 tctgttgtca aaaaaatgca gatcacccgc cagcaatgtc agcagatagc gaacggctgg
    15181 cgcggtcagt tagctatcgc agtggataat attgtcaggc cagaacgtac acggcagatg
    15241 atcgttgatt tttatcgcca ttttgacgac gtcgaacttc tggtctttca ggaagtgttt
    15301 aacggtgtct gggatgcgct ttccgatggg cgcgtggaac tggcgattgg cgcaacacgc
    15361 gcgattccgg taggcggtcg ttatgccttc cgggatatgg ggatgctaag ctggagttgc
    15421 gttgttgcca gccaccaccc gctggcgttg atggatggcc cgttcagcga tgatacgttg
    15481 cgcaactggc cgtcgttggt gcgcgaagac acctcgcgaa cgctacctaa acgtattacc
    15541 tggttgctgg ataaccaaaa aagagttgtc gtgcctgact gggaatcatc ggcaacctgt
    15601 atctcggcag gattatgcat agggatggtg ccaacacatt tcgccaaacc gtggcttaat
    15661 gaggggaagt gggtggcatt agaactggaa aacccctttc cggattcggc atgttgtctg
    15721 acatggcagc aaaatgatat gtcgcctgcg ttaacctggc tgcttgagta tctcggcgat
    15781 agtgagacgc tgaataagga gtggctgcgg gagccggaag agactcccgc aacgggtgat
    15841 taacgacgat agtcgcggaa cgggccgtca gccacggagc ggcgttcaat caagcgcgga
    15901 tgcacttcaa tggactgcgg ttcttcacgt ttgttgacga tacgatccaa cagcatgttg
    15961 aacgctgttt cacccagcga atcttttggc tggtggatcg tggtcagcgc cggcgtaaaa
    16021 tagcgggcgt tgcgcacgtt atcataaccg atcagcgaaa catcctgcgg gacgcgcaga
    16081 cccatttcat cagcagcaca aagtgcgccc attgccatga tatcgccacc acagaagacg
    16141 gcagtagggc gatgcggctg cgacagtatt tgctgcatgg cgcgataacc agattcgggt
    16201 tcaaagtcac cctgaacaat ccagctttcc ggcaccttga tcatcgcttc ttccatcgcc
    16261 ttcataaaac cggcaaggcg gcctgcgccg gtgttacgtt ccagcgggcc ggggatgacg
    16321 ccgatttcgc ggtgaccgcg ttcaatcaga taacgcccgg ccatgtagcc gccttcgaac
    16381 gcgttatcaa tgaccgcatc ggtgaagtca gcttttgctt caccccagtc catcaccacc
    16441 attgggatat ggcgatactc ttccagcatc gccagcaacg gctctgggta ctcagaacac
    16501 atcaccagca gaccatcgac gcgtttttgc gccatcatcg acagataagc ccgctgtttc
    16561 tcaagattgt tccacgcatt acccagaatc agggtgtaac ctttctggaa gcaatttttt
    16621 tcaaccgctt caatgatctc ggcaaaatag gccgcttcgc tgctggtcgc cagcaaaccg
    16681 atagacttgg tgtggttaac cttcaggcta cgcgccaccg cgctagggga gtagtgtaat
    16741 tctttaatcg ctgcccacac ggcgttgcgc gtttcttcag cgacgaaacg tgttttgttg
    16801 attacgtgtg acacagttgt agtggaaacg tttgctcgtt tcgctacatc ttttattgtt
    16861 gccattccat ttcactccag accctaactt cagctcctgc aaaatcgcaa ggtaaacgtt
    16921 tgcctttaca caccttacgc acaacaaaag tgttgcggta cgccggaaaa acgacacgga
    16981 gcgtcaggaa ggggtcaatg gccagtacgc aaatagtgta agcgtggaat tttggccgat
    17041 cttgacgaaa aggggaagtg gtaaaaacac ttatcattat aaatgacggg agattttttc
    17101 atcacagtgt gtaaaaatgc gtaatatcac tatttgtgtg gtaataaaaa aggagaaaac
    17161 ttgatgagca ccgaccttaa attttcactg gtaacaacga ttatcgtcct cggtttgatc
    17221 gtggccgtgg gtttgactgc cgcgctgcac tgatttcctc tcgagggagc gcgttctccc
    17281 tcgcatctcc ttcctgtttg ttagcaaaat ggcaaaagtg ttttctcttt ttgttatttt
    17341 catttttttg tgattgctgt catgctttcg ccactttaat gaatatcgcg taatgacgcg
    17401 aagaggtgga gataaggcat gaaaattaac tatccgttgc tggcgctggc gattggcgcg
    17461 tttggtatcg ggacaacgga gttctcgcca atgggtttgt tgcccgtcat tgcgcgcggt
    17521 gtggatgtct cgattcccgc tgccggaatg ttaatcagtg cctatgcagt tggcgtaatg
    17581 gttggcgcgc cgctgatgac gcttctactt tctcatcgtg cccgccgcag tgcgttgatt
    17641 ttcctgatgg caattttcac gctcggcaac gtactttccg ccatcgcgcc ggattatatg
    17701 accctgatgc tttcacgcat tttgaccagc ctgaatcacg gagcattttt tggtttgggt
    17761 tcagtcgtgg ccgcaagcgt ggtgccaaaa cataaacagg ccagcgcagt tgccactatg
    17821 tttatggggt taaccctggc aaatatcggt ggcgtgccgg cggcgacctg gttgggtgaa
    17881 accatcggct ggcggatgtc atttctggca acggcggggc tgggagtgat ttcaatggta
    17941 agtctgttct tctcattacc taaaggtggt gcaggggcac gacctgaagt gaaaaaagag
    18001 ctggcggtat taatgcgtcc gcaggtgctg tctgcattgc tgacgacggt actgggagct
    18061 ggtgcaatgt ttactctcta cacctatatc tctccggtac tacaaagtat tacccacgca
    18121 acaccagtgt tcgtcacggc aatgctggtg ctgattggtg tcggattctc tatcggtaac
    18181 tatctcggcg gcaaactggc agatcgttca gttaacggca cgttgaaagg ctttttgttg
    18241 ttgttgatgg tgattatgct ggcaatcccg ttcctggccc gcaatgagtt cggcgcagct
    18301 attagcatgg tggtgtgggg cgcagcaacc tttgcggtcg taccgccgtt acagatgcgc
    18361 gtgatgcgtg tcgccagtga agcgccaggt ctgtcttcat cagtcaatat tggtgccttt
    18421 aatcttggaa atgcgctggg agcagctgct ggtggtgcgg taatttccgc tgggctggga
    18481 tacagctttg tgccggtgat gggagcgatt gtcgcgggac tggcattatt gctggtgttt
    18541 atgtcagcca gaaaacaacc tgaaacagtt tgcgttgcta acagctaatt aaacaaaaac
    18601 gcagaaggat aaccttctgc gttacagttg ctgacaacgt gcgatttatc ttgccgaatg
    18661 cgctgttaac gcctcatccg cgttggcatg gtaagcgaag cgcatcaggc aatgctgcat
    18721 ttgccatcag ttattatgca gcgagatttt tcgctacgaa ttcccagttc accagcgccc
    18781 agaagtgctc cagatagcca ggacgtgcat tgcgatagtc gatgtaataa gcgtgttccc
    18841 agacatcaac ggtcagcagc ggagtcgcat cggtggtcag cggagtaccc gcgttagagg
    18901 ttgaaacgat agccagtttg ccatcgctgt ttttcaccag ccaggtccag ccagaaccaa
    18961 agtttttgat cgctgcatca gtaaactgcg ctttgaaatc ggcaaagctg ccaaaagatg
    19021 cggcgatagc ttcagcgact tttccagtcg gttcgccacc ggcgttcggt gccaggcagt
    19081 tccagtagaa agtatggttc cagacctgag ctgcgttgtt gaatacgcca ccttcagagc
    19141 tgcgaataat ctcttccagt gatttacctt caaacgcggt acctttaatc aggttgttca
    19201 ggttagtgac ataagtctga tgatgcttgc cgtagtgata ctcgatagtt tccgcagaaa
    19261 tgtgcggtgc cagagcatct ttagcatatg gtagtgcagg taattcgaat gacattgcta
    19321 ctctccttta ttattaattt gcatacgtac aatagcctta ttgtgcgtat ggatagggta
    19381 gcaaattaaa aggtgagaca aaagatgaac ttaccctgtt gccgtaacaa cagggtaaag
    19441 ttttagcgaa gtgtttttgg ggtcattacc cgacgagcgc caacatagtg gcgctgccag
    19501 tagtcttcac tgagagaagt gatttgaatt tcctgacctg tgcgcggtga ctgaataaat
    19561 ttgccgttgc cgacatacac gccgacatga tcggctgtgc cgcgtccctg agtacggaaa
    19621 aagaccaggt cgccgttttt cagttcacta cgttcgattg gcgctgcatc acgcaggtga
    19681 tacatttcat tcgccgtacg cggaatacga attttcacca aatctttata agcgtaataa
    19741 accaggccgc tgcaatcaaa accggtacgc ggtgagctgc caccccaacg atatggctta
    19801 ccaatttgct gcatcagttt attcattgcc actttcgtag ctttttgcac cttcgctttg
    19861 tgcgcgtcgg caagagttcc tgacgcggca tttttgactt tcacacaatg cgatttatag
    19921 cccttacgtt tggtacactt ttcggtaaag ctggctgagg ccgttttatt gacggcgtgt
    19981 ttgctgcgag ttttggcgtt tttagaagcg gtaatggaag attttttaac ggaagaagcg
    20041 gttttgcttt tggtcgtact tttactggcg gcttttttcg ctgttttctg ggtctttttg
    20101 gtggttgccg tgcttttctt tttatctgct tttttggtga tatgagtggt agcagaactc
    20161 tccctggctt gctttgaagc atgggccata ggcgttaaag gcagggtggt aaaaagtaaa
    20221 gcacagagcg tgatcgaaat acggtttatc cgcgccactg agcgtccctc tgttaaggca
    20281 ggtctatcat gcctgcgtct gatttacaaa acttgatgat tctaatgcat agaagaagag
    20341 aaagttaata gcttttattg ttaaaaaata cgtttcgttg gcgagcgcaa aaaaatgcag
    20401 cagtgaaagc taaaaatggt tataaatacg ccagtttgcc gcgcatcaga gaagaaacga
    20461 atatttgaaa tgcaacaaat agagcaaagg gataaaatag caaaagcgtg atgaaaatgt
    20521 tattttccga tgccgcactg acccgctaca atgtcagatt actgccgtaa agaagtttga
    20581 ggaagcaaga caatgagcac cactatcgaa aaaatccaac gccagattgc tgaaaacccg
    20641 atcctgctgt acatgaaagg ttcaccgaaa ctgccgagct gcggtttctc tgcccaggca
    20701 gtccaggcgc ttgccgcatg tggcgaacgt tttgcctatg ttgatattct gcagaatccg
    20761 gacattcgtg cggaactgcc gaaatatgct aactggccga ccttcccgca actgtgggtt
    20821 gacggcgagc tggtcggcgg ttgtgatatc gtgatcgaaa tgtatcagcg tggcgaactg
    20881 cagcagctga tcaaagaaac tgccgctaaa tacaagtctg aagagccgga cgcggaataa
    20941 tccgtcctgc aatactgacg gatgcgaacg ggcatccgtc agtcctttct ctatccccaa
    21001 tccagccctt gtggcgaact tgaaaatccc gcctcacgca gcagcgtaaa catcggcgtt
    21061 tgccggaccg gtagatcatt cacttctgtt agcgtaaagc gcagccgtgg ttcgcgacgc
    21121 agtgcggtag tcagcgcgtg gaaaacctct ggggcgagta attcctcttt ttcctgccag
    21181 accagcattt ttttgccacc ttgcgccaga tagagtaaca atttgccgcc agaaacgacc
    21241 accagcgcac ccgcccgacg cgttggaacc agcgttgccg gatgtgcagg ccagggcaga
    21301 agatttcccc acacattagc cggatcgttg gcagagagcg ccactggtgt atagtggcgc
    21361 gtttgcgtgg cttgtgtcgc cagatcgcgc aatcggtcaa tagtcagacg ttcagcgaat
    21421 tgcgcgccac ccagaccttc tacaaaacga cctcgcataa ttcgcccgga gtcttccata
    21481 cttcgacaaa gcgtttgcat cgatggaaac ccgccaggga tattttcggc tatcaccgcc
    21541 tgacgactga tgatgccgta gcggtcgagc atattttccg ccagcgccaa catcctttcg
    21601 gtatcgttta gtggctccac ctgcaataac gaccagcgtc cagccagatt tggtgtgttg
    21661 taagatacca gcggcgagac gggttgcgca tagacaggac gtccacgacg agcccggtga
    21721 ctgcggcgag ttgaggtgcg tgcgttggaa ctgctgcggg tgagggcgcg taacggtgcc
    21781 caaatgtcgc tggtgatgac gccttgccag accagcgccc ataacgcctc ttgcagggca
    21841 gagagatcaa ccgattcgcc gattttatcg cgtatccgtt ggctgatttg ttgtgcaaac
    21901 caggctcctc cgtcagccag aacagcgact atcgcttgtt gcagcgccga acgattcgcc
    21961 tgatccgctt cggcgggagt gaacgattct gcagcatatt cctgtagatg cagtgccacc
    22021 aggccgtcat cttcacccag ttttttttgc cccgaccaga taactgcacc ggttgccagt
    22081 aattcatcga gcatttctga cgaatagtcg cgtacgcggg caggcaggat ctggctttcc
    22141 cagagtgagg cgggtaaacc gactccggca agctgttcga tcacccgcat cacgccatct
    22201 acgccctcat aaacgcctgg cgatgttgag gcaaagagcg ccgggctacc atcggtggcg
    22261 ggtaatacgc cctgacgttc cagcagcaat cgcgcatagg tcgtggctgc aacgggacgc
    22321 gtcgcttctc tggcggcttg cagcgagcgc aaacgcagac gacgaaatac ttcatcgctc
    22381 acccagatgt cttgttgcag attcatcacc agaccctgtt cacgcagttg ctgaagctgc
    22441 tcttcgacaa tggcaatacc gagactaaac tcatgagcca gttgttcagc cgtgaccaga
    22501 gcatgagccc ggagatagcg cagaaagagg tcgcgaagcg ggtaactgac tctatgtaaa
    22561 taaatctctg gcaatgactc tggtagtcgt acgccgaggg catcacgcag cctggcggca
    22621 tcatccatac aggcaagacg ctcctgtccg ctaatcatcg ctgggaagat tcgttttact
    22681 gcaagaagat tttccagata cgacgcaacc tcttcactgc tgcctgtatg ccgttgcgcc
    22741 aggtcttcaa cggtcattgg ccccagttcg cgcagcaggt cgaacaatcc ttcttcacct
    22801 ttcgctcttc tgccaggagc cagtcgttgc aactcttctt ccacctggcg aatgacctgc
    22861 gggtcgagta attcccccgg atcgacctgt ccgagtagat tgcgcagtaa ctcgctgtcc
    22921 agcgacagta cggatgcccg gcgctctgcc agcggggcgt cgctctggta cataaattcc
    22981 gcgacatagc cgaacaataa acttgtggcg aaaggcgagg gcgtagtggt cgttacatcg
    23041 gatatttgaa tttcgccacc gttcaggcga cgcatcaaac gttccagtgc gggaagatca
    23101 taaacatctt gcagacattc gcgtagggtt tcgagaatga ccggaaaatc cggatatccc
    23161 tgagcgattt ccagcaactg actggcgcgc agccgttgtt gccaaagcgg ggtgcgatgg
    23221 cccggagtgc gccccggcat taataatgcc cgcgcggcgc attcgcgaaa acgggcggcg
    23281 aaaagtgccg agctgcctac cgcctcgcgg acaatttgca gcaacttttc tggttcaaac
    23341 aaaaaaatcg cggcgtcggg caatttacca tcagtgtcag gaatgcgcgc aacaatgccg
    23401 tcatcactgg cgaccacaga cgcgtcagcg ccccataggg catgtattcg cccggcaatc
    23461 gccagcgccc agggttcatg cacccgtctt ccatagggag agtgcaaaat aatacgccag
    23521 tcgccaattt catcacggca ccgttcgagg accagatggc gactccccgg aacgatgccc
    23581 gtcgcgttgc gttgctcgtc aatcaacccc tgaatattgg cgttcgtatt ttcttctgcc
    23641 agccacgggg gaatagtccc ggaaaagaac gcgccatccg ccagtaaatg aagaaaatcg
    23701 ccgatcatct cgcctaattc agccggacgt ccgttacctt caccacgcca gaaggggagc
    23761 cgggcagaac gacccggagc aggagtcaca atcacctgat cgcgggtgat ttgctggatc
    23821 cgccatgagg tagcgccgag agtgataatg tcgttcaccc gcgactcata taccatctcc
    23881 tcatccagtt cacccacccg ccgcgaaccg gcctgttctt caccttcggg taataacacg
    23941 ctatacatgc cacgatccgg aatggtgccg ccgctggtaa ccgccaacaa ttgagcgcca
    24001 ggtcgggcgg tcaatatccc ggtctccctg ttccagacca gtttggggcg aaaagcagaa
    24061 aaatcgccag agggatagcg cccggaaagc atatccagcg tggcgtcaaa aacacgtctt
    24121 ggcagatctt tccacggtgc ggcacggcgt acgcgggagt accattcgtc tacctgtaat
    24181 gcatccatcg ccgcggcggc aacggtttgc tgcgcaagga cgtcgagtgg attatgcggc
    24241 ggtgtcaggt tttccagcct gcctgcgaac atacactcta caatgactgc ggaatcgact
    24301 aagtcacgcc gggtacgagg gaaaaacagc cctttagata caccgccaac ctgatgtccg
    24361 gcgcgaccaa tgcgttgtaa cccactggca acagaaagcg gcgttgccac ctgaatcacc
    24421 agatccactg cgcccatatc aatccccagt tcgagactgg aggttgcgac cacgcagcgt
    24481 aactcacccg atttcagcgc ctgctcggtg attgctcgtt gttctttaga gacggagccg
    24541 tggtgtgagc gggcaataaa aacgtcgcta ctttgtacac ggttagaggt tgcgccggag
    24601 gtcgactcga aatgggccgc atcaacggcg atagacgggg aacgctgtaa gcgtgcggcg
    24661 taaagctcat ttaatcgtgc cgtcagtttt tccgccagcc cacgtgaatt agtaaagaca
    24721 atggtcgagc gatggcgcaa cacttcatca aggatacccg tttcaatata tggccagatg
    24781 gagccttccc ggccggcatg gctgtcttcg ccggtgctgc tggcgaccga tgagacatca
    24841 tccatattgg cgaccggtac gacaattcgt atctgcggat ggcgcattgc gggcgggttg
    24901 actaccgtaa ccgggcgatc gccaccaaga aatgctgcca catcgctggc tgagcgcaca
    24961 gtggcagaaa ggccaattcg ctgtgctgag gtgtggagca gcgcatcgag ccgctccaga
    25021 cttaacgcca gatgcgcacc acgtttactg cccgctaccg cgtggacttc atcaataatt
    25081 accgtttcga cgccgcgtag cgtttcgcgc gcgcgggagg tcagcatcag atagagtgat
    25141 tcgggtgtgg tgatcagaat atccggcgga ttacgggtga gtttgctgcg ttcctgtgca
    25201 ggcgtatcgc cagtacggat ccctacgcga agattgactt ccgtttcgcc gcgccgccgc
    25261 cgttcatcgg caataccctt caacgggagc tgcaagttgc gctgaacgtc ggtgcccaga
    25321 gcttttatcg gtgaaatata gaggatgcgt gaggttttac gcttatgcgc ctcgcgggta
    25381 tcttcgccgc cttcgcggaa gagccgatcg agggcgtaga gaaatgctgc cagcgttttc
    25441 ccggagccgg tcggtgcaat caccagcgca tgttcgcttc gcgccgccac atgccaggtt
    25501 tgcgactgga cagcggtcgg ctgtttaaag gcgcgaagaa accagtcgcg ggtcgccggt
    25561 gaaaacacgt ccggcaggag cgatgaaggg tctggattat ctgccattaa cgctattccc
    25621 cgtgaatatc atgcaggatg cgtggaatag cctcactata gatgcctgaa tcattgcatg
    25681 tcatcaggca tcgactcgat tacacctctt cggcggcagg tagcggccag cctcccagac
    25741 gtttccagcg gttgacgatt tcacaaaaca gcacagcagt gcgttcggtg tcgtacagcg
    25801 cggagtgcgc ctgggtgctg tcgaagtcca tgccagcggt ctggcaagcc tttgacaata
    25861 cggtttgtcc gagtgccagc ccggccagtg cagcagtgtc aaaagtggcg aaagggtgga
    25921 acgggttacg tttcagtgag gcgcgttctg cggcggccat cataaagctg tgatcaaaat
    25981 tggcattgtg cgccaccata atggcgcggt tacagccgct cgctttaata cctttacgta
    26041 caactttaaa aatttcgtgc agcgcctcgt attcgctgac cgcgccgcga tcgggatcgt
    26101 tcgggtcaat gccgttgaag gcgagggcct ctggctgcaa atttgcgccg acaaatggtt
    26161 cgacgtggaa atgtaatgtg gtgtccggca tcagccagcc ttgttcatcc attttcaggg
    26221 tgatggcggc aatctcaagc agcgcatcgg ttttggcgtt aaatccggct gtttcaacat
    26281 cgatcacaac aggataaaaa ccacgaaaac ggtcgcacag accggtaagt tgagcgttat
    26341 cggacatctg attctcttaa tacggataaa ttgcagcgcg cattatgaca aatattactt
    26401 gtcgatgcag taaagatgcg ggcgcgatga gttcacgccc ggcaggagat tagttgccca
    26461 gaccgcgacc ggcgtctttc tcttcgatta actcaatttt gtaaccgtcc ggatcttcca
    26521 caaacgcgat aaccgtagta ccgcctttta ccggacccgc ttcacgggtc acgttacccc
    26581 cgttttgacg gattttttcg cacgcttcag cggcgttatc tacgctaagc gcgatgtgac
    26641 cataagcagt gccgagttcg tatttatcca cgccccagtt gtaggtcagt tcaatcaccg
    26701 cttcttcggt ttccgggccg tagccaacaa acgccagtga gtatttgtat tccgggtttt
    26761 cgctggtacg cagcagtttc atgcccagca ctttggtata aaaatcgatg gagcgttgca
    26821 gatcgccaac gcgcagcatg gtatgaagaa gacgcatctt tttatcctca aaatggctaa
    26881 cagattcaaa atgttgtttt agtatagcgg cggctttacg ccgctcgcaa tgttggatta
    26941 caacgtcggg taatcggtat agccttccgc gccgccaccg tagaaacttt cggcacgctg
    27001 tgggttaagc tcagctttgc gctgcaagcg ggcgaccaga tccgggttcg caatccagtc
    27061 acgaccaaat gccaccgcat caattaaccc tttgccgatc agcgtttcag ctttttctac
    27121 tgtgtatgca cctgcgccga taatcggacc gtggaaacgg gcgcgtactt tttcgcggaa
    27181 cgcatcagta tacggttcac cccccgccca atctggttct gacatatgca gataagcaat
    27241 gccgcgttta cccagttgtt caatcagata cagtgcatcg gcttcttcat tcgggccgtt
    27301 atccgtgttc tggaaagtac cgattggcga aacgcgaatg ccaatgcgat cggcacccca
    27361 ttcttcaatc ccggcatcga ccacttccag taccagacgt gcgcgatttt ccacgctgcc
    27421 gccgtactga tcggtacgat ggtttgaaga aggagaaagg aactgatgca gcaaataacc
    27481 gtgagcagag tggagctcta ccagatcaaa accggcttca cgcgcgttag caatggcctg
    27541 acggaaatca ttgacgatac ctggaatctc ttccagttca agcgcacgcg gcatggatgt
    27601 ttcaacacgg atcgcctgac cattttcatc gcgcagagaa gtacgtgttc ccgcgctaag
    27661 tgctgaaggc gctaccggtg cctgaccgcc aggttgcagg ctggcgtgag aaatgcgtcc
    27721 ggtgtgccac agctgcacgg ccatatgacc attttcagca tgaacgccag cggtgatttt
    27781 tttccatgcg gcaatttgct ccggactatg gatgccaggc gcacctgcat atccttttgc
    27841 ctgggcagaa atttgcgtgg cttcactaat aatcaaaccg gcactggcac gttggcgata
    27901 gtattccgcc atcaacgggg taggaatgtc acccggttca atactgcgca gacgcgtcag
    27961 cggtgccata aaaatacggt ttgccgccgt gatcgcgccc actttcagtg gggaatacag
    28021 tttttcagat gacataaagg ctcccggtcg tctggtgata aaaaatgcct gctaaacggc
    28081 aggcgtcgca ataatgtttt ttacatgggc cagcgcgttt tccagtggct cgaaactgcg
    28141 cgaaattttg gcctgcagat tcgcgccaag ccacagtgcg taaagcactt gtgcctgttg
    28201 tagcggttcg ccacaaaagg ttaaacaatg gttctcacgg ccattttcca gcgcctgaga
    28261 gagcagggcg atcacgccgc gagcaccttt atccatcgcg ctgcgcatat cttctgacag
    28321 atcgcacact tcggcagaga gttttactgt caggcaacca ctgatggttc catgttggca
    28381 aaactggttc agtgtttgct ggtaataagc cagtatgcgg tcgcggtagt taccttcgcc
    28441 ggattgcagc aactcagtca gtcgctggtg atatgcggcg taatgacgct caagcatggc
    28501 aacgccaaac gcttctttag agcgaaagta gtgatagaag gaccctttcg gcacttcagc
    28561 ggtttttagt aattcgctta gccccatccc ggtgaatcca cgttgcaggc aaagttgctc
    28621 gcccgtcgcc aggagatgtt cgcgagtatc atgttcggtg tgtttgttca tgcgttggag
    28681 tgtagtagac cagtcggtct agtgcaagca aagttgctac tttggacatt aacagcttca
    28741 ataatggtaa taatcttatc tgaggagggc gaagtggcgg agcaattaga gttctttccc
    28801 gtccagagcc cgtgccgggg aatttgccag tctgacgaac gcgggttttg tcgcggctgt
    28861 tttcgtagtc gtgatgagcg ttttaactgg aataaaatga gcgatggcga aaaacaagag
    28921 gtgctgcgcc tttgtcggca gcgattaatg cgcaaattac gcgcaaataa gccagcgtca
    28981 tcagacgaac cgaagcaacc atcactcttt tgaacctaat cttgcgtata ctcctctcat
    29041 atctgataag aggaagcgat tatggttcag cgtattacta ttgcgccgca aggcccggag
    29101 ttttcccgtt ttgtgatggg ctactggcga ttgatggact ggaatatgtc cgcccgccag
    29161 ctggtcagtt ttattgaaga gcatctggat ctcggcgtga ccaccgtgga ccatgctgat
    29221 atttatggtg gctatcagtg cgaagcggcg tttggcgagg cactgaaact ggcacctcac
    29281 ctgcgtgaac ggatggaaat cgtcagtaaa tgcggtatcg cgacgaccgc gcgtgaagaa
    29341 aacgtcattg gtcattacat cactgaccgc gatcacatca ttaagagcgc cgaacagtcg
    29401 ctaattaatc tcgcgaccga tcatctggat ctgctgttaa tccaccgacc agacccgtta
    29461 atggatgccg atgaagtggc ggacgcgttc aaacatctgc atcagagcgg caaagtgcgt
    29521 cattttggcg tatcgaactt tacgcctgcg caatttgccc tgttgcaatc acgtctgccg
    29581 tttacccttg ccactaatca ggtggaaata tccccggtgc atcagccgtt actgctggat
    29641 ggcacgctcg accaactaca acaactgcgt gttcgtccga tggcgtggtc ctgccttggt
    29701 ggtggtcgtc tgtttaatga tgattatttc cagccgctgc gtgatgaact ggctgtggtg
    29761 gcagaggagt taaacgcggg ctcgattgaa caggtggttt acgcctgggt attacgttta
    29821 ccatcgcagc cgctgccaat tatcggttca ggtaaaattg agcgcgtacg ggcagctgtc
    29881 gaagcagaaa cactgaaaat gacccgtcaa caatggtttc gtatccgtaa agcggcactg
    29941 gggtacgacg taccgtaagc acttttagga atagccgccg ttcaaaaatg tgtcactggt
    30001 ttacacttat tcaggaatgc acaatgaacg gaggtcctat gaaacgtttt agtctggcta
    30061 ttctggcgct ggttgttgca accggcgcac aagctgccag tgaaaaagtc gagatgaacc
    30121 tcgtcacgtc gcaaggggta gggcagtcaa ttggtagcgt caccattact gaaaccgata
    30181 aaggtctgga gttttcgccc gatctgaaag cattaccccc cggtgaacat ggcttccata
    30241 ttcatgccaa aggaagctgc cagccagcca ccaaagatgg caaagccagc gccgcggaat
    30301 ccgcaggcgg gcatcttgat ccacaaaata ccggtaaaca tgaagggcca gaaggtgccg
    30361 ggcatttagg cgatctgcct gcactggtcg tcaataatga cggcaaagct accgatgccg
    30421 tcatcgcgcc tcgtctgaaa tcactggatg aaatcaaaga caaagcgctg atggtccacg
    30481 ttggcggcga taatatgtcc gatcaaccta aaccgctggg cggtggcggt gaacgctatg
    30541 cctgtggtgt aattaagtaa ttacgaggcc agcgtacctt gcggtggtgc ttgctcaagt
    30601 tgcgaaagcg agcagtacag ccgccagaca attgccgcca gctcacgggc ggcaggttga
    30661 tgatgacggg caaggctgtc gcaaatccgc tgtaattctt caagtgtggc cgccagtgat
    30721 ttttgctgaa cgccacgctc actcatcact ccccgcaaca gtgaaataca gttatcccgt
    30781 actcgcgata acggatcgga acgcgattcc cagtcgcgca attgccagac aacatgagaa
    30841 cagttcagca gcactacacc ccagcgtaat aaccaacggc gagccagcgc atcctggctg
    30901 ttactcaact gactgacgtg atgataagtg agcgattcaa attcgctttc actcagtgtt
    30961 ggatggcggc ttagctgatc gacaaaatcc cggcgcagcg cgcgaatatg gcggcggctt
    31021 ttacgagcat ccgatcctgg acgcagaatg gcgaacgcta gccaagccag cgcaacgcca
    31081 acgatttttg ccagattatc gttaagaaaa tcagcaaaat catacaccgg gggattagtg
    31141 acagcgataa acgaacccat aaaaacaatc agttgccccc acagtgcggc aaatttgggc
    31201 atctgcaatt taagaagctg cattgtcgcc agcagtggaa agagaaacag taaaaattgc
    31261 cacagatcgc taatctggac catcaggcca aatttgacca caaagctgaa tagcgaaagt
    31321 aacaccagcg tgcgcatcag cagtgacaac gacttaaacg gtgctgcgac ggcggagtag
    31381 agtacgcagc taattgctgc cagcgttaat gcattggcac cggcatccca ttgcgatgca
    31441 atactccatg cgccaatcat catcaacgta caaaatgtac gcagcccgct ccacatagct
    31501 tcggcgttgt cggtatgacg agccagagcg gatgtccgtg cgagtctggc atgatcatct
    31561 acaccgcttt gcagacgatg taattcctga ctactttgca gataaaggcg gcaaaaataa
    31621 cgtagtcgct gccagaaggc gacgtgccgg tagtcggcga cgttggtcgg gcgtagcggg
    31681 gcgataatac gtgcgacggt gtaagcatct gtttgcgaac tggcgagcgc cgtcagcaat
    31741 tgttcgagaa tttctcgcgt ggcacctggc ggtgagggcc agttgagcaa catacgtcgc
    31801 aggctggaga tgacactggt catacgtaat tgctggtgga gtagcgcatt aaggcgcgcg
    31861 ttttgctggc gaaaacgata gtggctccag aaagcctgga tacgcagcaa attcatggtc
    31921 agtatctgcc caatcacccc ctcatgtgct gcacgaatgg catcgtttgt ttcaggctgc
    31981 cagagtaaac tggcatgttc cagtaatcgg gcgtgcatgt ttttcaatgc ggttaaaagg
    32041 gctgtagcat cggaactgct aggcaggatc atcatcatca tgccgccgca caaaataccg
    32101 actatcacct cgcaaacgcg cgcctgagcg atatcccaca gctggctggc ctcagtaata
    32161 ttaaccatcg gaaaggctat gatggcagcc gtgtagcccg ccagttgaaa tgcatacgcg
    32221 acgttattcg tgaagtgcgc acaggcccag gtacaaaagc caagccacgc cgacatgctc
    32281 aatagaaaaa accacggctc attgagcgta tgcccggcaa gaagcagtgc cgcaatggct
    32341 cccagtaaac tgccagcgat gcgtccgagg cttttgctga tgacaccgcc aacggtggga
    32401 aagctaacca ctgcagccga ggtcatcgcc caatagggtt catccagatt caaataatag
    32461 gcaaccgtca gcgccagaca catggcaatg gtattgcgta acgcataacg ccattgcgcc
    32521 agcgtggcct tgaaccaggg caaattgcgc aaggaccatg acgatgcgtt cattattgac
    32581 ctacggcaat gctacaggtg gtgcccgaca ccagcgtgac atcgcctgga actttatcca
    32641 gcgcaaagcg gacgggaacg cgttgggcga gacgaaccca ggggacgtta ggtttcacat
    32701 ccggaataag gcttgagtca ctttcaacgc tttgatcata aatcgcccga ccgatactgg
    32761 aaacgtgacc ccgtaacgtt ttgttatcac tatagagggt aatttgtgct ggtgcacctt
    32821 cgcggatatg gcgcaacttg gtttcttcaa aataaccaat gacgtaaaac gagtggctgt
    32881 cgacaagggc aaacagcggt ttcccggtgt cagcgtaatc gccgatgcgg gtagtgaggt
    32941 tagtcaccca accactcacc ggggcgcgaa tttccgtttg cgccagttgc cattgtgtct
    33001 gtttcagcgt ggcctgtgcg gcattaacac tggcctgcat cgctttaaca ttgaggttag
    33061 cggtatcgag ttcttcggca gaaataaaat tttgtgagag atgacgacga cgattcgctt
    33121 cgttattggc ttttgcgagg tcagattgcg ctttcgccag ctgagcctgt gcgttcagct
    33181 cggcgatctg aaagggcgtt ttatcgatcg tgagcagaag atcccccgcg ttaaccagtt
    33241 ggttgtcttt tatattcagc tcaacaatac gtcccgacac ctgtggggtg atagaaacct
    33301 tttctgcgcg tattttcccg tcgcgtgtcc acggagattg catgtaataa ttccacagcc
    33361 accatccggc aagaaccgca actatcgcta caatgattgt tgagaaatac ttaattgttt
    33421 taattgacat agttaccacg caatcagtat tgccagagca aggcaaacgc aaatcgcaaa
    33481 cagcgataaa tccattaaca agggatgcca gatgtcaccg gcgtagatcc agccacgaag
    33541 caggcggtgt acgacaagcc agatgacgaa tccaaacgcg aaggctttga aaaacggagg
    33601 aaaatagacg gacgcaccga acaccagatc ttgtaagggc aatcctgttg cattgagcat
    33661 aaacttcaca aagtagattc ctttacgacc gaatatattg cgttagatta ataaatattc
    33721 tttaagtgcg aaaaatttac gcgcaatttc tgaaaaatat gtctgacggt aaccaaatgc
    33781 agcaatacat ttgttttagc aatacaattg ctgcacacta ttctaaaagc cgcataatat
    33841 cttagcaagc taattataag gagatgaaat tggaatcgcc actaggttct gatctggcac
    33901 ggttggtgcg catatggcgt gctctgatag accatcgcct gaaaccgctg gagttaacac
    33961 aaacccattg ggttacgtta cacaatattc atcagttacc tccagaccag tcgcaaattc
    34021 aactggcaaa agcgattggc atcgagcagc catcactggt ccgtactctg gaccaactgg
    34081 aagaaaaagg gttaatttcg cgtcaaactt gtgccagcga tcgtcgggct aaacgtatta
    34141 aactgacgga aaaggcagag ccactgatca gcgaaatgga agctgttatt aacaaaaccc
    34201 gcgcggaaat attacatggc atctccgcag aggaactgga gcaactgatt acgctcatcg
    34261 caaaacttga gcataatatc attgagttac aggccaaagg gtgaaatgaa gggggcgtgt
    34321 ggtcagtaac ctgaccacac gcaaacttat ttagcgcgga gaaacggtca cctgactgcc
    34381 attgctggcc agtacgacac gttggcccgg agagaaacga gtgttgcctt gtttctgtac
    34441 caccatgatg gtattaccat cgtctttacg aatttccagc tcgacaccct gcgttttgtt
    34501 cattgcactc tgtacgccct gaccagctac gccacctgca acagcgcctg ctgcagtagc
    34561 cagagaacgc ccggttccgc caccaacagt attccccagg aaaccaccaa gaacagcacc
    34621 gccaattgca ccgataacgt tggaatcatc accgccctga atctgtaccg gacgtacgtt
    34681 aacgatggtg ccatagctga cattctgtac ttgtttcgct tcagaagcgg tataaacatc
    34741 ccctgacagg gtgtcgttat taacacaacc gacaagagac agacctacca ttgaaacaac
    34801 caatacgcgt ttaatcattg aaaaatctcc tgttcaccat gaaacgccac gcaagcatcc
    34861 ctcatggtca aagtatatgg catatgtgaa tccatggtta catgttctac caaatatgag
    34921 caaatcataa tgaaagtagg atcaaccaat tataaacaaa acattcatga tgattattga
    34981 tttgaagata atgttttact accaacggta aaaatcggtc attccgcatg gtattcacga
    35041 aaagtttatg tttgagtggt gcgcaaacgt cagattgaaa aaggatgaac tatgaaatcg
    35101 ggccgcttta ttggcgttat gtcaggcacc agccttgatg gtgttgatgt tgtgttggcg
    35161 acaatagatg aacaccgggt cgcacagctg gcaagcttga gctggccgat cccggtgtct
    35221 ctgaaacagg ctgtactgga tatttgccag ggccagcagc ttacactttc gcagtttgga
    35281 cagcttgata ctcaactcgg gcgacttttt gctgatgcgg tcaatgcctt gcttaaggaa
    35341 caaaacctgc aggcgagaga tatagttgcg atcggctgtc acggtcaaac cgtctggcat
    35401 gaaccgacgg gcgtggcacc acacacttta cagattggcg ataacaatca aattgtggca
    35461 cgcaccggaa ttacggttgt cggtgatttt cgccgtcgcg atattgcctt gggaggacaa
    35521 ggcgcaccgc tggtacctgc gttccatcat gccttgctgg ctcactcaac cgagcgacga
    35581 atggtgctca atattggcgg catcgccaat ctgtcactgc tcattcctgg gcagccggtt
    35641 ggcggctacg ataccggtcc tggtaacatg ctgatggatg cctggatctg gcgtcaggcc
    35701 ggtaaacctt acgataaaga tgccgagtgg gcacaggcgg gtaaagttat tctcccactg
    35761 ctgcaaaata tgctcagcga cccatatttc tcgcaacctg caccgaaaag caccggacgc
    35821 gaatacttta actacggctg gctggagcgc catttacgcc attttccggg tgttgatccc
    35881 cgtgatgttc aggccacact ggcagaactc accgccgtga ccatttctga acaagttttg
    35941 ttgagcggtg gctgcgaacg attgatggta tgtggtggag gtagtcgtaa tccgctactc
    36001 atggcgcgtc tggcggcatt actgccaggc acagaagtca ccaccaccga tgccgttggc
    36061 attagtggcg atgacatgga agcattggct ttcgcctggc ttgcctggcg gacgctggcg
    36121 ggattaccag gaaatctgcc ttccgtcact ggcgcaagcc aggagacggt actgggggct
    36181 attttccctg ctaacccgtg atatcagagt taactgaatt tttcttaccg cgtctgccgt
    36241 tacacttgga acgttaggga gggcgtattg ccctccagac caggaaagtc ttcgggaaat
    36301 gtctatgaaa aaactgttac tgatctgttt gccggtgctt ctcaccggct gtagcgcgtt
    36361 caatcaactg gttgagcaca tgcaaaccgc tacgctggaa taccagtgtg atgaaaaacc
    36421 gttgacggtc aaactgaata atccgcgcca ggaagtcagt tttgtttacg ataatcaact
    36481 actgcatctc aaacagggca tttcagcgtc tggtgcacgt tacactgacg gaatctatgt
    36541 tttctggtcg aaaggcgatg aagcgactgt ctataaacgc gaccgcatcg tattgaataa
    36601 ctgtcagtta caaaatccac agcgttgaga tttttccagg ggcggcgcac aatagcgtca
    36661 cccactgaca atccgtaaag aaaaccatgt ctgataacga cgaattgcag caaatcgcgc
    36721 atctgcgccg tgaatacacc aaaggcgggt tacgccgtcg cgatcttccc gccgatccat
    36781 taaccctttt tgaacgttgg ctctctcagg cgtgtgaagc caaactggca gaccctaccg
    36841 cgatggtggt cgctaccgtg gatgaacatg ctcagcctta tcaacgcatc gttttactca
    36901 aacattacga cgaaaaaggc atggtgtttt acaccaacct cggtagccgt aaagcacatc
    36961 aaattgaaaa taatccgcgc gttagcctgc tgttcccgtg gcataccctt gaacgccagg
    37021 tgatggtgat cggtaaagca gaacgacttt cgactctcga agtgatgaaa tattttcata
    37081 gccgcccgcg tgatagccag attggtgcat gggtttcgaa gcagtccagt cgcatttctg
    37141 cccgcggtat ccttgaaagt aaattcctgg agctgaagca gaagtttcaa cagggcgaag
    37201 tgccattgcc gagcttttgg ggcggttttc gcgtcagcct tgaacagatt gagttctggc
    37261 agggcggtga gcatcgcctg catgaccgct ttttgtacca gcgagaaaat gatgcgtgga
    37321 agattgatcg tcttgcaccc tgaaaagatg caaaaatctt gctttaatcg ctggtactcc
    37381 tgattctggc actttattct atgtctcttt cgcatctggc gaaaagtcgt gtaccggcaa
    37441 aggtgcagtc gttatataca tggagatttt gatggcaagc agtaacttga ttaaacaatt
    37501 gcaagagcgg gggctggtag cccaggtgac ggacgaggaa gcgttagcag agcgactggc
    37561 gcaaggcccg atcgcgctct attgcggctt cgatcctacc gctgacagct tgcatttggg
    37621 gcatcttgtt ccattgttat gcctgaaacg cttccagcag gcgggccaca agccggttgc
    37681 gctggtaggc ggcgcgacgg gtctgattgg cgacccgagc ttcaaagctg ccgagcgtaa
    37741 gctgaacacc gaagaaactg ttcaggagtg ggtggacaaa atccgtaagc aggttgcccc
    37801 gttcctcgat ttcgactgtg gagaaaactc tgctatcgcg gcgaacaact atgactggtt
    37861 cggcaatatg aatgtgctga ccttcctgcg cgatattggc aaacacttct ccgttaacca
    37921 gatgatcaac aaagaagcgg ttaagcagcg tctcaaccgt gaagatcagg ggatttcgtt
    37981 cactgagttt tcctacaacc tgttgcaggg ttatgacttc gcctgtctga acaaacagta
    38041 cggtgtggtg ctgcaaattg gtggttctga ccagtggggt aacatcactt ctggtatcga
    38101 cctgacccgt cgtctgcatc agaatcaggt gtttggcctg accgttccgc tgatcactaa
    38161 agcagatggc accaaatttg gtaaaactga aggcggcgca gtctggttgg atccgaagaa
    38221 aaccagcccg tacaaattct accagttctg gatcaacact gcggatgccg acgtttaccg
    38281 cttcctgaag ttcttcacct ttatgagcat tgaagagatc aacgccctgg aagaagaaga
    38341 taaaaacagc ggtaaagcac cgcgcgccca gtatgtactg gcggagcagg tgactcgtct
    38401 ggttcacggt gaagaaggtt tacaggcggc aaaacgtatt accgaatgcc tgttcagcgg
    38461 ttctttgagt gcgctgagtg aagcggactt cgaacagctg gcgcaggacg gcgtaccgat
    38521 ggttgagatg gaaaagggcg cagacctgat gcaggcgctg gtcgattctg aactgcaacc
    38581 ttcccgtggt caggcgcgta aaactatcgc ctccaatgcc atcaccatta acggtgaaaa
    38641 acagtccgat cctgaatact tctttaaaga agaagatcgt ctgtttggtc gttttacctt
    38701 actgcgtcgc ggcaaaaaga attactgtct gatttgctgg aaataatgca ttaagtggaa
    38761 agggggagtg agaaatcact ccccctggtt tttatacagg gaacatgatg aaaaatattc
    38821 tcgctatcca gtctcacgtt gtttatggtc atgcgggtaa cagtgcggca gagtttccta
    38881 tgcgccgcct gggcgcgaac gtctggccgc tgaacaccgt tcaattttct aatcacaccc
    38941 aatacggcaa atggactggc tgcgtgatgc cgcccagcca tttaaccgaa attgtgcaag
    39001 gcattgccgc cattgataaa ttacacacct gtgatgccgt gttaagtggc tatctgggat
    39061 cggcggagca gggtgaacat atcctcggta tcgtccgtca ggtgaaagcc gcgaatccgc
    39121 aggcgaaata tttttgcgat ccggtaatgg gtcatccgga aaaaggctgt atcgttgcac
    39181 cgggtgtcgc agagtttcat gtgcggcacg gtttgcctgc cagcgatatc attgcgccga
    39241 atctggttga gctggaaata ctctgtgagc atgcggtaaa taacgtcgaa gaagcggttc
    39301 tggcagcgcg cgaactcatt gcgcaagggc cacaaattgt gttggttaaa cacctggcgc
    39361 gagctggcta cagccgtgac cgttttgaaa tgctgctggt caccgccgat gaagcctggc
    39421 atatcagccg tccgctggtg gattttggta tgcgccagcc ggtaggtgtt ggtgatgtga
    39481 cgagcggttt actgctggtg aaactgcttc agggggcaac gctgcaggag gcgctggaac
    39541 atgtgaccgc tgcagtctac gaaatcatgg tgaccaccaa agcaatgcag gaatatgagc
    39601 tgcaagtggt ggctgctcag gatcgtattg ccaaaccaga acattacttc agcgcaacaa
    39661 agctctgaaa tatctcattt cggcccacaa cgacctgtgg gccgatggat attactttaa
    39721 gccttccgct gacagcgcgt cttgtacttc cggacgttca gccatacgtt gcataaatgc
    39781 tgcaatgtgc tctaaccctt ccagattcag tttcaccgcg tatgcccagc gcagaaccgt
    39841 aaacagatag gcatcagcaa ttgtaaatct ttgcccgcag atccagtgct catccttcag
    39901 tgcctcgttc acatattgca gcttcttctc cagttgcgcg cgaactgtcg gtttgtactc
    39961 ttccggtgta tcagggcgaa acagaggtgt gaaaccttta tgcagctcgg tggcgatgta
    40021 attcagccat tcgatggttt tatagcggga aatactgttt accggtgcca gcaactggcg
    40081 gtcggggacg ctgtcggcaa gatactgcat aatcgctacg ccttccgtca gcaaagtacc
    40141 gtcatccagc agcaatgcag gcacctgccc cttagggtta acggcaaagt aatcgtcacc
    40201 gttttcgaga cgttttttca ttaaatccac actgacgagg gtaaaatcct ttccgctctc
    40261 acgcagggtg atatgggaag cgagagagca ggcacccggt ttgtagaaca atttcatcgg
    40321 taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt gctgaacaaa aaaaaagccg
    40381 ctaacgaaaa gttagcggct tgtaaaagag tttccctgaa ttttacgcta cagcagcttt
    40441 cgccgctttg tctgcagcgt catcctgcgt catacggtgc agtttaggcg cggtcagcag
    40501 catcagtact gcaataatgg cagtagcgac accaatctgc aagaatacgc gaccataaac
    40561 ttccagtgac atcagcgggt cggtaacgtt atccggcaca gccatcatac ccgcaacata
    40621 accaccaatc aggtttgcac cggcagtggt caggaaccag ctacccataa tgaagcccat
    40681 cagacgctgc ggaacgagtt gtgcaaccat tgccagaccc agaccagaga tcatcagttc
    40741 cccgatgctc tgcaggccat agcttgcgac cagccagctt acagacacga taccagcgtc
    40801 agacgcgaat ttcgcaccca gcggcagaat caggaacgca ccagaacaca tcaccatgcc
    40861 gattgcaaac ttggttggca tcggcagggt atcgcccatc ttgttataga tagcggccag
    40921 aatcggacta ccgatgatga tccagaacgg gttcagtgcc tgatactgct caggttctac
    40981 ggccagaccc agaatggagt gctcaacgtt acgaatcgca aagaagttca gtgacgttgg
    41041 catctggctg tacagcacga agaagataat ggcttcgagc atcaggatga aggcaacgat
    41101 cattttacga cgcgcagcac ctttcatcgc gaaggcttct ttaccgaaga taaccacgat
    41161 accgaaggca acaacgccca gcgccatacg cgcaacttcc tgattgtgca gcagccaggt
    41221 ggcgatagcg atcagtgcca caacaccaat aatggtcagc agcaggttac ggtagttgat
    41281 aggctcgaag tctggttttg aaccgtactg tttaacccag cgttggcaga aggcgaagtt
    41341 aacgatagtg atcagcaggc ctacaacgct caacgcaaac gcaacactcc agccgtattt
    41401 cgcggccagc cacggcgtag caatcataga gaagaaagag ccgatgttga cggacatgta
    41461 gtacatggtg aatgcaccgt ccagacgcgg gtcgtttttc tcatagcatg tagaaagcag
    41521 agaagacggg ttagctttaa acaggccgtt accgaccgca atagccgcca tacccatata
    41581 aacgataccg gcgtcgtgac cagaccaggc aaccagagca taaccaatcg ccagcacaat
    41641 agcgccgagc ataattacgc gtttagtacc cagtaccttg tcacctaacc agccgccgat
    41701 agcgaccaga ccataaacca gggcactaaa ggaagagaaa agggtgattg aatccgcttc
    41761 agacataccc agttgtttaa ccaggtaaac agccataatt ccttgtaggc cgtaataacc
    41821 aaaacgttcc cataactcaa tcgagaagat gagatagaac gccttcggtt gtttgaaagc
    41881 gttcaaactg acgctttcag ttggtttttg gtttgcagtg gacactttta cctctttttt
    41941 tacatcccat attaacgggg gtgctttctc gcgttatgtc taaaaaacat acgcgcaatt
    42001 gttatagtgg gaggggaaac ggcaggtaat gttcactatc ttgcactttc agacaagtcc
    42061 tttgtaataa tctgttacat ataactgagg cggggaattc aaccattccg ggaaatgtta
    42121 tccagcgtta aatttttcaa aaaacgtaac ggcagatttt ttcactgcac tgagaggggg
    42181 gtgatggctt aacggcgata tgttctgcta tttagcgttt ttactagtga gatagtccag
    42241 tttctgaaaa atagccagtg taatgttttg taggtcaata tttgttgcgt gtgtaaattt
    42301 aaatgatttt aacctgattc ttgcaatgtt tttacattgc gacagcatcg tgatcttgat
    42361 cacgaatgaa tagaaaattt cgctattaaa aaaacaatta acctgcattt ttggttatcc
    42421 gcaaacggat tacctgatga taacgctaag caatggcatc attcacgtta aaaagatgtt
    42481 ttaaaagagg ataaagaaag gttatcaatg gggtaatcgg tgttacccct tttcttcaga
    42541 tgtcaacttt ctctttgtat tcacaaagat cttcaataat acaagagcca cagcggggct
    42601 tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca atggtggcag tcgactttaa
    42661 actctgccgg aaccactttc agtagctttt cttctacctg ttcgacgttt ttccccggcg
    42721 caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc gacagcaata gtcggccagc
    42781 cgaatgcagt gtttaatacg acgttggctg ttttacgacc tacgccgggc agggcttcaa
    42841 gcgcagcacg atcttccgga acctcgccat tatactgctc cagtaagata cggcaggttt
    42901 tgatgatatt ttctgctttg ctgttataaa gcccaatcgt tttgatatag gttttaaccc
    42961 cttcaacgcc cagttcaagc atcgctgcag gcgtattcgc caccgggtaa agtttcgccg
    43021 tcgccttatt aacactgaca tcggtcgcct gagcggaaag cagtacggca atcagcaatt
    43081 caaaaggcga actgaaatta agctcggtgg tgggatgagg attgttctca cgcaggcgag
    43141 tgaggatctc caggcgtttt gctttgttca tcagacattc cctgtttcac cgtttggcaa
    43201 tgcacgttcg gcagctgctt cagtacggcg ctttttcatt ctttcatcaa tcaggtattt
    43261 tcctgccagc atcagtccca ggccaataaa tgcacctgga ggcagcatcg ccagcaggaa
    43321 aggggagtcg gtgtggaaaa tttccacgcg taatactttt gcccagctac ctaacagcgc
    43381 atctgcaccg tcaaacaatg tgccattgcc gataatttcg cgtagtgaac ccagcacgaa
    43441 catggcgcag gttgcgccca taccaattga aaagccgtcc agtgccgaaa gcgccggacc
    43501 ttttttggcg gcgaaggctt cagcgcggcc cacaacgata cagttagtga caatcagcgg
    43561 aataaaaatc cctaatgatt gatacaggcc aaaggcgtag gcgttgatca gcatctgtac
    43621 agcgctgacc accgaggcga tgatcatcac gtaaatggga atgcggatct cggctggcgt
    43681 ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc agtaccagcg tagtcgcaag
    43741 tcctaaaccc agagcgttag tggcagtgga cgtgaccgcc aacagaggac aaaggccgag
    43801 cagctggacc agcgcagagt tgtttttcca caacccctga acaataacgt ctttaatttc
    43861 gctcacgctt tattctccac aggcaggaag ttgagaaagt tgtgccggta acgtctgagc
    43921 gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg ggagtaatcg tcgcgccggt
    43981 gaactggtcg aaatcaccac catctttctt caccgcccag tgcgcatcat ctgcaccact
    44041 gattttttta cccgcaaaat gggtgatcca gtcagaaagg cgcagttcga ttttatcgcc
    44101 aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca agtaccgtgc cgttaaaatc
    44161 ggctcccacc agcagctgaa tcgcaccgga atagccatct ggcgcggttg cttccagaac
    44221 ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc cgatgctcac ctttacctaa
    44281 ctcttgcgca gttaccagat agcaactctg tgccagcgca ttgttatagc gttcggctgg
    44341 cagcacctga tcaaataacg ccttttgttg cagactggcc tgttcagcaa tcgtcgtttt
    44401 ggtcatctgg ttgatggccg cagttaaccc tgttgaaccc gctgcaaata gcgccaacgt
    44461 aatgccgtgt tttcggatag ttttcagcat ggtttaccct ttgcgatggc cgtagacgcg
    44521 cggacgcgtg tagtaatcga tcagaggaac cgtgatgttc gccagcagga cggcaaaagc
    44581 cacgccgtca ggatagccgc cgaaactgcg gatcaaccag actaataagc ccgccagcgc
    44641 gccgaaaata agacgaccac gattggtcgt agaagcggta accgggtcag tcaaaataaa
    44701 gaatgcgccg agcatggtcg caccagacag cagatgaatt tgcggtgctg ccagtgtttc
    44761 tggtgagaac aaccagccca acgttgcgca taacgccagc gttactaaga agctgagggg
    44821 aatatgccag cgaatcgctt tctgccatag caaccacagg ccgccagcca gccaggcgag
    44881 atttacccat tgccaaccag cgcccgccag aataccgctg tagatcggat attgcataat
    44941 ctgttcaacc gaatgaccgg cacggacaga ggttttaaat gtatccagcg gtgtcgcctg
    45001 actaatgcca tcaataccta agcgtagtgt gttcatatca ccaccactgg cggtatgtcc
    45061 gctgaaaata acctgtatgg cgtcgataaa accagggatg ttgaccgcaa tttcatgtgg
    45121 cggtaaccag ctggtcatct gcaccgggaa ggagatcagt aagaccacat aaccaatcat
    45181 tgccggatta aacgggtttt gccccagacc gccatacaac tgtttagcga tgatcaccgc
    45241 aaacaccgta cccagcacga ccatccacca tggcgcgagg gggggaatac ttaccgccag
    45301 caataagcct gtcagcaatg ctgagttatc tttcaacgtt gcggctaccg actgcttgcg
    45361 tagtttgagt acgagagctt cggctaacag agcactaacc gatgccaaca ggatctgaac
    45421 gagagtaccc caaccaaaaa accacagttg cgctgcgatt cctggcacgg ctgcgagcaa
    45481 caccaacagc ataatgcgcg atgtctggcg ctggttatgg gtataagggg agctagctat
    45541 tctgaatacc atttagtcct cgtttacaac cttctgctgg gcggcttttt tggcctgagc
    45601 gcgggcaata gccgcggcaa ctgccgcttt gcgcggatca acctgttctt ctggttctgc
    45661 gttagtctgt tgctgttcca gcttgcgcac tttggcacgg gcaatagcgg cttcgacggc
    45721 ggctttgcgc ggatcaacct gttcttctgg ttccgcatta gcctgttgct gttccagttt
    45781 gcgcgctttg gctcgggcaa tagcggcttc gacggcggct ttgcgcggat caacctgttc
    45841 ttctggttcc gcattag
//
LOCUS       DAZGSP010000032        45452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZGSP010000032 DAZGSP010000000
VERSION     DAZGSP010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.032"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            79..621
                     /gene="yfaZ"
                     /locus_tag="ACHK74_001926"
     CDS             79..621
                     /gene="yfaZ"
                     /locus_tag="ACHK74_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFP0220755.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            721..1923
                     /gene="yfaY"
                     /locus_tag="ACHK74_001927"
     CDS             721..1923
                     /gene="yfaY"
                     /locus_tag="ACHK74_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFP0220756.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            1987..2448
                     /locus_tag="ACHK74_001928"
                     /pseudo
     CDS             1987..2448
                     /locus_tag="ACHK74_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(2489..3391)
                     /gene="rpnE"
                     /locus_tag="ACHK74_001929"
     CDS             complement(2489..3391)
                     /gene="rpnE"
                     /locus_tag="ACHK74_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFP0220757.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLGRQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(3585..4775)
                     /gene="glpC"
                     /locus_tag="ACHK74_001930"
     CDS             complement(3585..4775)
                     /gene="glpC"
                     /locus_tag="ACHK74_001930"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFP0220758.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(4772..6031)
                     /gene="glpB"
                     /locus_tag="ACHK74_001931"
     CDS             complement(4772..6031)
                     /gene="glpB"
                     /locus_tag="ACHK74_001931"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFP0220759.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(6021..7649)
                     /gene="glpA"
                     /locus_tag="ACHK74_001932"
     CDS             complement(6021..7649)
                     /gene="glpA"
                     /locus_tag="ACHK74_001932"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFP0220760.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            7922..9280
                     /gene="glpT"
                     /locus_tag="ACHK74_001933"
     CDS             7922..9280
                     /gene="glpT"
                     /locus_tag="ACHK74_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFP0220761.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            9285..10361
                     /gene="glpQ"
                     /locus_tag="ACHK74_001934"
     CDS             9285..10361
                     /gene="glpQ"
                     /locus_tag="ACHK74_001934"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP0220762.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(10403..10642)
                     /locus_tag="ACHK74_001935"
                     /pseudo
     CDS             complement(10403..10642)
                     /locus_tag="ACHK74_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10640..10744
                     /locus_tag="ACHK74_001936"
                     /pseudo
     CDS             10640..10744
                     /locus_tag="ACHK74_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10824..11474
                     /gene="inaA"
                     /locus_tag="ACHK74_001937"
     CDS             10824..11474
                     /gene="inaA"
                     /locus_tag="ACHK74_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFP0220763.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(11528..11782)
                     /gene="yfaE"
                     /locus_tag="ACHK74_001938"
     CDS             complement(11528..11782)
                     /gene="yfaE"
                     /locus_tag="ACHK74_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFP0220764.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(11782..12912)
                     /gene="nrdB"
                     /locus_tag="ACHK74_001939"
     CDS             complement(11782..12912)
                     /gene="nrdB"
                     /locus_tag="ACHK74_001939"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP0220765.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(13001..15286)
                     /gene="nrdA"
                     /locus_tag="ACHK74_001940"
     CDS             complement(13001..15286)
                     /gene="nrdA"
                     /locus_tag="ACHK74_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP0220766.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            15414..15557
                     /gene="ypaB"
                     /locus_tag="ACHK74_001941"
     CDS             15414..15557
                     /gene="ypaB"
                     /locus_tag="ACHK74_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFP0220767.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            15982..19716
                     /gene="yfaL"
                     /locus_tag="ACHK74_001942"
     CDS             15982..19716
                     /gene="yfaL"
                     /locus_tag="ACHK74_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFP0220768.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAQFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVVDAGDGQVSLANN
                     NSYLGTTQIASGMLEVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(19844..20566)
                     /gene="ubiG"
                     /locus_tag="ACHK74_001943"
     CDS             complement(19844..20566)
                     /gene="ubiG"
                     /locus_tag="ACHK74_001943"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFP0220769.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            20713..23340
                     /gene="gyrA"
                     /locus_tag="ACHK74_001944"
     CDS             20713..23340
                     /gene="gyrA"
                     /locus_tag="ACHK74_001944"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFP0220770.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            23489..25177
                     /gene="yfaA"
                     /locus_tag="ACHK74_001945"
     CDS             23489..25177
                     /gene="yfaA"
                     /locus_tag="ACHK74_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFP0220771.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            25174..25797
                     /gene="yfaT"
                     /locus_tag="ACHK74_001946"
     CDS             25174..25797
                     /gene="yfaT"
                     /locus_tag="ACHK74_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFP0220772.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            25821..30335
                     /locus_tag="ACHK74_001947"
     CDS             25821..30335
                     /locus_tag="ACHK74_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFP0220773.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLIEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENLFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLGDA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            30336..31985
                     /gene="yfaQ"
                     /locus_tag="ACHK74_001948"
     CDS             30336..31985
                     /gene="yfaQ"
                     /locus_tag="ACHK74_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFP0220774.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            31990..32766
                     /gene="yfaP"
                     /locus_tag="ACHK74_001949"
     CDS             31990..32766
                     /gene="yfaP"
                     /locus_tag="ACHK74_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFP0220775.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            33041..35890
                     /gene="rcsC"
                     /locus_tag="ACHK74_001950"
     CDS             33041..35890
                     /gene="rcsC"
                     /locus_tag="ACHK74_001950"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFP0220776.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(36090..36740)
                     /gene="rcsB"
                     /locus_tag="ACHK74_001951"
     CDS             complement(36090..36740)
                     /gene="rcsB"
                     /locus_tag="ACHK74_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFP0220777.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(36785..38074)
                     /locus_tag="ACHK74_001952"
     CDS             complement(36785..38074)
                     /locus_tag="ACHK74_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0220778.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKKEITIKALSEKWLELKKMEITAN
                     AMVRYKSAIRNCLDCLGGNKLASSITQEDVLIFRKELLTGYQLPGKNQRKPIKGRSVP
                     TVNSYMTIISGMFRFAANNGYIGKNPFSELPSLKRAKTPPDPLTKDEFARLIDACHHQ
                     QTKNFWSLAVYTGMRHGELCGLAWEDIDLEAGTLTVKRNYTQTKEFTLPKTDAGTNRV
                     IQLIQPAIDVLKNQATLTRLGKQYQIEVKLREYGRTSTHSCSFVFNPQILDRTGNSGV
                     HYAVTSINRTWESALKRAKIRHRKAYQSRHTYACWALTAGANPNFIANQMGHANAQMV
                     YNVYGAWMKDNNQSQVEVLNRKLCTYVPQVPQRLNKA"
     gene            complement(38108..38362)
                     /gene="xisR"
                     /locus_tag="ACHK74_001953"
     CDS             complement(38108..38362)
                     /gene="xisR"
                     /locus_tag="ACHK74_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP0220779.1"
                     /translation="METIIYQITPSKWCTERTLIAATGLKSGTIARARKKSWLLGKEY
                     RHYAAEGEPGPFSECVYNIEEIMRWIENQKQPGAKNASSG"
     gene            complement(38370..38516)
                     /locus_tag="ACHK74_001954"
     CDS             complement(38370..38516)
                     /locus_tag="ACHK74_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220780.1"
                     /translation="MNREFEIWIRLRYGYRYDLTRDAHGFYCREVVKRMFDVWCHCRG
                     LKIV"
     gene            complement(38537..38779)
                     /locus_tag="ACHK74_001955"
     CDS             complement(38537..38779)
                     /locus_tag="ACHK74_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220781.1"
                     /translation="MGEMVDINDELVRTVITVDDGCDHTQRFIWMMNTRRNIRNKVFR
                     HEPKPVRVTKIRIEARKRRKYRPRKARAPAQPNELA"
     gene            complement(38772..39674)
                     /locus_tag="ACHK74_001956"
     CDS             complement(38772..39674)
                     /locus_tag="ACHK74_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220782.1"
                     /translation="MKEADLKALRDAAQNTVYGDKGCDLEAFHSLATPPVILALISHI
                     ENLEKKLDEIVTSEISPKAWLNNLTGEVCSIDAMPDARNDRETYTPLYPAWQRIAAAD
                     DNGRVAWMYTHKKAGYRVPTMTSWESLAEERGGSLDKYIVSPLFTVPQIPPVRTEQEA
                     YPDVQVSWDATRHYVNGWNACRQAMLTTAEKVLDVRIPEYIGRWLSAALHDQNSCEEM
                     KNDIRRFLAEYHHVPSKYNGWRLVPDKLTEQMKYRIVPLLATIKPRSVDEYDRSGENA
                     QFLEWVWTQLMEATKFAYEGSVNG"
     gene            complement(39671..39895)
                     /locus_tag="ACHK74_001957"
     CDS             complement(39671..39895)
                     /locus_tag="ACHK74_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220783.1"
                     /translation="MIISFTDYGEQAKLIITSSRLTLRKHNRVVDAVLLCTPDITESR
                     SGFFLVKSSLSGKTRDILRAYKTATRESER"
     gene            complement(39912..40274)
                     /locus_tag="ACHK74_001958"
     CDS             complement(39912..40274)
                     /locus_tag="ACHK74_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFP0220784.1"
                     /translation="MNSTTAAIMNYDPNMTLCGRMARQTVRLTLGQWEYRETFEVDVV
                     GNLTGLDVITCAIENLYEDLPYEEAQDEYTGETNIMATIQIGDLACTDEDMLGEAWLA
                     GMLIAAEIISIEPAGTLS"
     gene            complement(40267..41133)
                     /locus_tag="ACHK74_001959"
     CDS             complement(40267..41133)
                     /locus_tag="ACHK74_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFP0220785.1"
                     /translation="MKKSTELVPVAARDLQIIEYRGQRVITTEQLAAGYGATPIRIRQ
                     NHSRNEERFVVGKHYFLLEGDELRELKHRVSQSYSVKIASNVRSLILWTERGAANHAK
                     LLETDQAWNYHEDLVEFYFTQRENIDVQRVLTRKELALMVIEAEERAEAMALENKTLS
                     ATVDSLEKHFSKGMTVPQFCRALNGVNTSQIMWWLSGRKWVFNEQRDPEKTPRWRTAS
                     YARDRYLTEEAVQITPHGKATFTKFTPVLLEKGCHRLYQFYMNGELPMKKNWNGEFKH
                     DKAIYTPEAEHE"
     gene            complement(41157..41741)
                     /locus_tag="ACHK74_001960"
     CDS             complement(41157..41741)
                     /locus_tag="ACHK74_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220786.1"
                     /translation="MTATEQLFFLTAEGIHTPYGLLDINKALEMLDEGCFDEPIWHGL
                     EIVCCISEAEEKGMTGELAPEKTIMYWRWLYTAMFFSEQLTRNGRTEVLNENGGTDKG
                     TMYRGHNGGMTIYLKPLRLAMQNYMEWALIETYGAESGLLKSILMYKAFTGALGDGEK
                     TGITMEGYRAMHMILDGLISEATDNSEPETRVFH"
     gene            complement(41728..42288)
                     /locus_tag="ACHK74_001961"
     CDS             complement(41728..42288)
                     /locus_tag="ACHK74_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220787.1"
                     /translation="MKEEPEIIEINSEEFTENEWLTETDPRVLALTMEGYIVFPPFFS
                     DTEYSAINNAGIDRAFHKYELDCGPVYRVCRHGQVKKCFTRKTAIIHLANFMTTKVFE
                     SSGFEKRLPDQRLMHPEYGEVFHPGEITPEYYRAHGRCFHRLLRILARRKQFDAWMEK
                     YNAWIDQYCRLVAQRPFKKRANHDSN"
     gene            complement(42696..43058)
                     /locus_tag="ACHK74_001962"
     CDS             complement(42696..43058)
                     /locus_tag="ACHK74_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220788.1"
                     /translation="MKDEYISAYRRRITVAALRRMKRKTGSNLLTVKRHDGELTTIEI
                     TEQFISQLLLRFEGVTRGEFGRKDGETAIRTAYQDAVGINQHGEYLTESGKLIIDELL
                     NECIDYIKEGHITGCVNH"
     gene            complement(43233..43433)
                     /locus_tag="ACHK74_001963"
     CDS             complement(43233..43433)
                     /locus_tag="ACHK74_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220789.1"
                     /translation="MKTPLIMLEEVAAEIKENTSMLEFIFKNSGDNGETDDFLLCLIR
                     SMNKTCEKAYEYVDALRTNKGN"
     gene            complement(43430..43621)
                     /locus_tag="ACHK74_001964"
     CDS             complement(43430..43621)
                     /locus_tag="ACHK74_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220790.1"
                     /translation="MKMFKGLTNEPKTAFHHIAVLLEAGLIISASGDEECDELSDDIF
                     LLAQQYARSACDAFKEQRT"
     gene            complement(43623..44000)
                     /locus_tag="ACHK74_001965"
     CDS             complement(43623..44000)
                     /locus_tag="ACHK74_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0220791.1"
                     /translation="MSTSQLMLIEQVESLLHESLESVSERNRHVLLEVGIEKIQQLKH
                     ILLPPRNKKDCRDGIRTQRESCGLSTAELATLLGVDEETIIQWENGKAEPLASQVIPL
                     ANILDCDPMWLLTSFHSDTTKES"
     gene            complement(44086..44745)
                     /locus_tag="ACHK74_001966"
     CDS             complement(44086..44745)
                     /locus_tag="ACHK74_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFP0220792.1"
                     /translation="MDHDSFGGRLLMRRVQLGMSQDDLAKLTGVSRVTISKIELGDSQ
                     DTRSSNLFRIADALKCSPKWLLDGSAPSPTDSIQNVRNPKPVSPNRYRYPKLTWVSAG
                     PWNCEDYIAYPEEWIGSDIYAGDHGFWLEVKGDSMTSQNGEFSIFEGMNILVNPDKEV
                     FSGKYVVARLKNSGEATLKQYIEDAGQCFLKPLNPRYPLIPINGDCEIIGVVVEMRMQ
                     I"
     gene            45085..45378
                     /locus_tag="ACHK74_001967"
     CDS             45085..45378
                     /locus_tag="ACHK74_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220793.1"
                     /translation="MSPDYVQMEMPARYSQADSEWIKRQLQSLPSSLRQKVALKYAEV
                     YEVTFDSEPVSFRKENRARHEANVRLRRFVETHGRALQGYTTQPPLAGTQQRT"
BASE COUNT        10798 a        11026 c        12062 g        11566 t
ORIGIN      
        1 tcattgaatc ctctgtgcca ggcagtacaa tcccgcagct ttgggaattt tgattaattg
       61 cttgtgggaa aggggataat gaaaaaaatt gcgctagcag gtctggccgg aatgctgttg
      121 gtttctgcat cggtcaatgc aatgagcatc agcggccagg cgggtaaaga atacaccaat
      181 attggtgtcg gttttgggac tgaatcgacg ggcctggctt taagcggtaa ctggacacat
      241 aacgacgacg acggtgacgt cgcgggcgtg gggctggggt tgaatctgcc tctcgggccg
      301 ttaatggcga ccgttggcgg aaaaggcgtt tacaccaacc cgaattacgg cgatgaaggt
      361 tatgccgcag cggtaggagg tggtttgcag tggaaaattg gcaacagctt ccgtttgttt
      421 ggcgagtatt actactctcc ggattcgctc tccagcggta ttcaaagtta tgaggaagcg
      481 aatgctggcg cgcgttacac cattatgcgt ccagtcagta ttgaggcggg ttatcgctac
      541 ctgaatctgt cgggtaaaga cggtaaccgc gacaacgccg tggctgacgg cccgtatgtt
      601 ggggttaacg ccagtttctg atcccttccg gcgcggcatt ctgtcgcgcc attcagtttc
      661 ttactacgcc ttatctccct ttgcgttata gtgattcgat aacaaaagca ggagataacc
      721 atgttaaaag tggaaatgct atccaccggg gatgaagtgt tacacgggca aatcgttgac
      781 actaacgctg cctggctggc cgattttttc tttcatcagg ggctgccatt atctcgccgc
      841 aatacggtgg gggataacct tgatgactta gtcaccattc ttcgcgaacg tagtcagcac
      901 gccgatgtgc tgatcgttaa cggcgggctg ggaccgacca gcgatgattt aagcgcactc
      961 gccgctgcga cagcaaaagg tgaaggcctg gtgctgcatg aagcctggct caaagagatg
     1021 gaacgctatt tccacgaacg tggacgagta atggcaccga gcaaccgtaa acaagcggag
     1081 ctgcctgcca gtgctgaatt tatcaataac ccggtaggca ccgcctgtgg ttttgccgtg
     1141 cagcttaatc gttgcctgat gttctttact cccggcgtac cgtcagaatt taaggtgatg
     1201 gtcgagcacg aaatcctgcc gcgtctgcgc gagcgttttt ctttaccaca gccgccggtt
     1261 tgtctgcgtt taactacctt tggtcgttcg gaaagcgatc tggcacaaag cctggacact
     1321 ctacaactgc cgccgggcgt aacaatgggc tatcgctctt caatgccgat tatcgaactg
     1381 aaactcaccg gaccggcaag cgagcaacag gcgatggaaa aactgtggct ggacgttaaa
     1441 cgtgttgccg gacagagcgt gattttcgaa ggcaccgaag gactgcccgc gcagatcagt
     1501 cgcgaattgc aaaaccgcca gttcagcctg acgttgagcg agcaattcac cggtggttta
     1561 ttggctttgc aactttctcg cgcaggtgct ccattgctgg cgtgtgaagt ggttccttca
     1621 caggaagaaa ccctggcgca aactgcgcac tggattacag aacggcgggc caaccatttt
     1681 gccgggctgg cactggctgt ttcgggtttc gagaacgaac atctcaactt tgcgctagcc
     1741 acaccagacg gcactttcgc tctgcgtgtg cgtttcagca ctacgcgcta cagcctggct
     1801 atccgtcagg aagtgtgcgc aatgatggca ctgaatatgc tgcgccgttg gttaaacggc
     1861 caggatatcg ccagtgagca tggctggatt gaggttattg agtccatgac cttatctgtc
     1921 tgattccgcc actctgtgcc ggtttactgc accggcacat tccccctccg gcaaaacttg
     1981 tccaaccctc cctacggtga gcgtggtgtt ggggccagtg tggcacgggc tgcgcgctgg
     2041 ggacgcattg agaattacat ggcgcaagtt aacgattcac tttgtctgtt ggtgcaggtg
     2101 gaaagtaaaa cggcactgga taacctggac gagatcctcg acgtcgaagg gattgatggc
     2161 gtgtttattg gaccagcgga tctttctgcg tcgttgggct acccggataa cgccgggcac
     2221 ccggaagtgc agcgaattat tgaaaccagc attcggcgga tccgtgctgc gggtaaagcg
     2281 gctggttttc tggctgtggc tcctgatatg gcgcagcaat gcctggcgtg gggagcgaac
     2341 tttgtcgctg ttggcgttga cacgatgctc tacagcgatg ccctggatca acgactggcg
     2401 atgtttaaat caggcaaaaa tgggccacgc ataaaaggta gttattgata tcaaaggccc
     2461 atggggatcg gctgtgggcc tgtgttaatt agtggttatt cgctgccaga tcggcttcgc
     2521 ttagctgggt ggccgcgagc acctggtcac gatcaatgcc ctgttccagc atgcgcaaag
     2581 caattttaat ggcttgttca tgcatacctt cctgccagcc aatttgatgc ccttcctgcc
     2641 gtagccgttc agcaatagtc attaatctct ccttttgtag tggtgaacgt tcggcaatct
     2701 cctcaataaa acgggtgaaa cgggaggtat cgccgcattg cagcagataa ttaaacagtg
     2761 tttgtagctg gctgtcatta gtgaagcctc taaccaaaag cgtggtgatc ctgtcaacca
     2821 tgccgattaa atcgcggtcg cgaatatgct tttgaatcag ttccagcaga gctatacgcc
     2881 gatgttgcat gatctcatcg tcaggtacga tggtgatatc caccaacgga aaagcttcgg
     2941 tgtacaactg ccggccaagt tgcggatcgt caaactcatc cagccagtta agtgagtacg
     3001 ggtagggcga tgtttcgcca tgataaaaca gcaatggcac caccagcgga actctgtcat
     3061 agcctttatc caggtgacgc tgcatggcgg cagtggcata gcgcattagc cgaaaagcca
     3121 tattcttttc tgcagagctt tgatgttcaa tcacgcagta gatatagccg tcaccgtcgc
     3181 tggtttccac ggaccacaaa acatccgagt agtaagcgcg taaacttttt tcaataaaac
     3241 tagtgggttc caggcgtaag gtttgcaggt tgcaaagctt gcgcagtggt tctggtaaat
     3301 gtatttcgag aaaatcccgt gcggtttcgg gtgtgaacat aaaggtttta aacaccgcat
     3361 catgcgggga ggatgttgtt gattcggtca tggttgtccg tcacctgata tttatgatga
     3421 cgcgacatta cgtctatccc gggcctttga catctgtcag tttcagcctt tgcgagacat
     3481 tttcagagga attttcaaag cgttgcaacg catacatggt gcgtggaatc gcgctcgcaa
     3541 aaatgcccga taagcaatgt gcttaccggg catcaaaagg tcggttaagc cagcgcctgg
     3601 gccagtagcg taatcggatg ttcgcagcga aggctcgtgg acatctcaat ctgccatttg
     3661 caggtttcgc agtcggtgat gaccagatcc gcgccgctct cttctatctg gcggaacagt
     3721 ggcgcaccga tggcttgtga ggtggggtag ttctcttttt tgaaaccgta agtacccgca
     3781 ataccgcagc actgggaatc cagcaccgtt aactcaagcc ccgggatttt acgcaacagc
     3841 tccagggtgt agagcgtcca gcccattttt tccatatggc acggagtgtg ataaaccact
     3901 ttcagcggca gcggtttcag cggtaacgtt ttgccttcgt ccagcttgcg ccacagccag
     3961 cgggttgcca gttcgatatg atcgcgcaag cctttgttgt cgacattcag cacttccggg
     4021 tattcgtcgc gcagggcaaa tgtacaggtt gaggaggtgg caatcaccgg aatgcctttt
     4081 actcccacag cttcgcggat cgactctaca ttcgtaattg cctgtttgcg tgctttatcg
     4141 gtaaagccgt tggcgattag cggtacgccg cagcattttt ctttgctgag cagttgtaca
     4201 ccggtaccca ttgcgttgag cactttaatt aaatctttac ctaactgcgg atggttgtag
     4261 ttaacgaagc agccgtgaaa gaaagcgacc tggtctttat attgtgcttg ctgagccgct
     4321 atgctgcgat accagcgacg gaacgtgccg aaggagtatt tcggtagcgt gcggcgatga
     4381 tcgattttta acgccgcatc aagcagctgc cgcaccggtt tcagcgaggt agcggtgttg
     4441 acgattggtg cgaacggcgt ggaaacgcta cccatcaggt cggtatgact caacacaaaa
     4501 ttacgcagcg acgggcgcgt ggtgtcatat tttgcccgcg cgcgctggat aatatcgcca
     4561 atcttcacat cggacgggca ggcgacttca caacgtttgc agttgatgca atatttcagc
     4621 gcctcgtcat acagtgcgcc atctttcaaa cgcagacgct cgccatccgg cccggcttgt
     4681 tttggccctg gataaccggg attcacccgg ctcaccgggc aggcggtggt gcagacggtg
     4741 cacttaatgc agttttcgaa gctggtgtcg ttcattgttg gcctcctgcg cgttgggcaa
     4801 tctgttgagc ggcatgtaaa gcactgacgg cacaaacacc gccgccgcat ccctgggcga
     4861 tgggatcaaa tccgcccagc accgaaccga tggcaaacag gttctcaatg gtttgccctg
     4921 cctgcgacgg gcgtagcgtc tcatcagtgg ttacaccgaa ctgctgccac ggttgcggcg
     4981 caaaaaaatc gcccttatac cattcacccc gcgtggcggt ttgtagcaca tcaaggccga
     5041 gaatcggctc gcgaatgccg ttacgttccg ctaccagtcc gccactaaag aaactgccgc
     5101 tggcgagaac cgcgaaacgt ggacgtagcg gaatatcggc gtgattgcgg gtccagattt
     5161 cattcactac gccattttta caggtcactt ttttcacttc atcgcccggc atccacacgc
     5221 caccctggcg cacaaactgg cgctgtaact ggttttgcag acgaatgccc agcacggaag
     5281 gcggcagcgt tggcaaaagc atcagtgaac aaggtagttt ttcattcaac caacgccaca
     5341 gtttgtcatc ggccaggccg aagcaggcgg gcatcaggat catttcgcag gtattggcga
     5401 caggaataag cgcatcaagt aacagcggcc agttttcttc attatcaagg aaacgggcga
     5461 tattcaccgc gcgaaattcg gtggcgttat tgcgcaggac atccagttcc ggcagctcta
     5521 tttctgcggt ttcaaccgaa agatccagtt cgcgcaacga agccgccgca agatgcgcct
     5581 ggaaatccat cagcccgctt atccccacta cacatatctt cttcgcgggt agcggccaga
     5641 cgggaacttc tggcgaactt aaccaggttg cacgcaaagt gccgagcggg gtaacgcgct
     5701 gatgggccag ttccacgctg ccctgcaatt gcgcaccgct ttcagcgatc aatgcctgcg
     5761 cctggcaagc gagatcgagc acgcgttgtg gttcgagaag actgtaagga tgggctggtg
     5821 cctgctgacg caaagattcc agtccactgt gaatgtctgt caccggttga ccatcaggca
     5881 gatggctcag caaatccagc gaaccggatg agaaatgcag cgcgctttga ccacgcgtga
     5941 caatggcaca gcgcaggccg tgtttttgca gttgcaggcc acagagtaat ccggcgaggc
     6001 cgccgcccat aatgacagta tcaaagcgca tctttctgct ccttctccag accacacaat
     6061 ccctgataaa cccagcgggt aaattcgctt tcgcgcagtg catctcccca ggcgatgggt
     6121 tgcacgcctt tccagcgttc gttaaggaag gtggaaagtt gctcgataga ttgcgcggac
     6181 gtcgtgacgt taaaacgttg cagcagtccg gcagcgcggc aggcgcagag ttcgccctgg
     6241 caggtaccca tccccacacg ggtacgacgg cgtaaatcca gcaggctatt aacgtttaaa
     6301 ttttctaccg cgtactgaac ttcacccgca gttaccgctt cgcactcaca taccaggcta
     6361 cgatgcagac ggccttcgct cagccaggca ggcgtgcgat cgccatgacg ataaaccgca
     6421 gaaccgcgca gcggggcagg cagggagatg actttacgca aggtaacttc agcgggttct
     6481 tgtgaaccag gcagtgccag atcggcagtc gtacaggggc gcgtgttgcc cagtttgcgg
     6541 cataccgcgt cggtagccca ttcagccatc agccgatagg tcatcagttt gccaccggtg
     6601 atggtgataa atccgtccag accatcgcgt tcagcatggt cgagcagcac gatgccacgg
     6661 ctgacgttac gtccgctcgg gtcgtcatcg ctggcaacca gcgggcgcac gccagaatag
     6721 gcccgcaaaa tgcgcgtttt cgccatcacg ggggccagtt tttccccttc acgcagcaga
     6781 atatcaacct cttctgccgt cactcgatta tcgtcaatct cgttgtaatc aatacgtaaa
     6841 gaggtggtgc caatcagcga aatggtatcg ccaggcacca gaatatcggc gtcggaaggt
     6901 ttacggcagc ggttgatcac atgctggtta atgcggtgat ccatgatcag cagcgatcct
     6961 ttcgccggga acatgcgaat gcgcagatcg gcatattcgg caatgtgttg cccccagatc
     7021 ccagcggcat taaccacgac aggtgcatga agggcctgag tttcgccggt gagatgatta
     7081 cgtacacgaa caccgcacac cgtcgcgcct tcacgaatca gccccgtgac ttcatgagcg
     7141 gtaaggataa cggcaccgtg ttctttggca tccagcatgt ttgctgcggt cagacgaaat
     7201 ggatcaacgg tgccatccgg aactttcacc gcgccaatca gtgccgggtt aacggcaggt
     7261 tcgataatgc gcgcttgctg cgggtctata gcttctgcgc tgatccctgc ttcttcgcag
     7321 gcgcgaataa aagtggcctg gaatgagagg tcatcttccg gcagggtgat aaacaggccg
     7381 ttggttggtt caacgcagtg acgtgcaatg cgtttcagga tctggttttc actaatgcat
     7441 tcgcgggccg attccgcatc ggttaccgca tagcgcgcac cgctgtgcag caggccgtgg
     7501 ttacgcccgg tggcaccggt tgcgatgtcg tggcgctcaa ccaaaatcac gcgcagcccg
     7561 cgcagggcac agtcgcgggc aatcccggct cccgttgcgc cgccaccaat gataatcacg
     7621 tcacttgatt gcgagtcgcg agttttcatt gttatccctc tgaagttcgt tttttaccat
     7681 ttagccatag taaaaacatg aattgtttga tttcgcgcat attcgctcat aattcgaaag
     7741 tgaaacgtga tttcatgcgt cattctgaac attttgtaaa tcttatttaa taatgtgtgc
     7801 ggcaattcac atttaatttc tgaatgtttt cttaacatcg cggcaactaa agaaacggca
     7861 ggttctccca ctgaatcagt ctgttaatca taaataagac cacgggccac ggaggctttc
     7921 aatgttgagt atttttaaac cagcgccaca caaagcgcgc ttacctgctg cggagatcga
     7981 tccgacttat cgtcgattgc gctggcaaat tttcctgggg atattctttg gctatgcggc
     8041 ttactatttg gttcgtaaga actttgcgct tgctatgcct tatctggtcg agcagggatt
     8101 ctctcgcggt gatttaggtt tcgcgctttc ggggatctcg atcgcttatg gattttcgaa
     8161 attcataatg ggttcggtat cggatcgctc gaatccgcgc gttttcctgc ccgcaggttt
     8221 gattctggcg gcggcagtga tgttgtttat gggctttgtg ccatgggcga cgtcgagcat
     8281 tgcggtgatg tttgtactgt tgttcctctg cggttggttc caggggatgg ggtggccgcc
     8341 gtgtggtcgt actatggtgc actggtggtc gcagaaagaa cgtggcggca ttgtgtcagt
     8401 gtggaactgt gcgcacaacg tcggtggtgg tattccgccg ctgctgttcc tgctggggat
     8461 ggcctggttc aatgactggc atgcggcgct ctatatgcct gctttctgcg ccattctggt
     8521 ggcattattc gcctttgcga tgatgcgcga taccccgcaa tcctgtggct tgccgccgat
     8581 cgaagagtac aaaaatgatt atccggacga ctataacgaa aaagcggaac aggagctgac
     8641 ggcgaagcaa atcttcatgc agtacgtact gccgaacaaa ctgctgtggt atatcgccat
     8701 cgccaacgtg ttcgtttatc tgctgcgtta cggcatcctc gactggtcac cgacttacct
     8761 gaaagaggtt aaacatttcg cgctggataa atcttcctgg gcctacttct tctatgagta
     8821 tgcgggtatt ccgggcaccc tgctgtgcgg ctggatgtcg gataaagtct tccgtggcaa
     8881 ccgtggggca accggcgttt tctttatgac actggtgacc atcgcgacta tcgtttactg
     8941 gatgaacccg gcaggtaacc caaccgtcga tatgatttgt atgattgtta tcggcttcct
     9001 gatctacggt cctgtgatgc tgatcggtct gcatgcgctg gaactggcac cgaaaaaagc
     9061 ggcaggtacg gcagcgggct ttaccgggct gtttggttac ctgggcggtt cggtggcggc
     9121 gagcgcgatt gttggctaca ccgtggactt cttcggctgg gatggcggct ttatggtaat
     9181 gattggcggc agcattctgg cggttatctt gttgattgtt gtgatgattg gcgaaaaacg
     9241 tcgccatgaa caattactgc aaaaacgcaa cggaggctaa tggcatgaaa ttgaagctga
     9301 aaaaccttag catggcgatc atgatgagca ctatagtcat gggaagcagt gcaatggcgg
     9361 cggacagcaa cgaaaaaata gtcatcgccc atcgcggtgc cagtggatat ttgccggagc
     9421 atacgttgcc agcaaaagcg atggcgtatg cgcagggagc ggattatctg gaacaggatt
     9481 tggtgatgac caaagacgac catctggttg ttctgcatga ccattatctc gatcgtgtta
     9541 ctgatgttgc cgatcgtttc ccggatcggg cgcgcaaaga cggtcgttac tacgcgatag
     9601 atttcacgct ggatgaaatt aagtcgctga aatttaccga aggtttcgat attgaaaacg
     9661 gtaaaaaagt acagacttat ccggggcgtt tcccaatggg taagtccgac ttccgggtgc
     9721 acacctttga agaagagatt gaatttgttc aggggttaaa tcactctacc gggaaaaata
     9781 tcggtatcta tccagaaatt aaagcgccgt ggttccatca tcaggaaggg aaggatattg
     9841 cggcaaaaac gctggaagtg ctgaagaaat atggttacac cggtaaggac gacaaagttt
     9901 atttgcaatg ttttgatgct gatgagctga agcgtattaa gaatgagctg gaacccaaaa
     9961 tgggcatgga tctcaatctg gtacagctga ttgcctatac cgactggaat gaaacgcagc
    10021 agaaacagcc ggatggaagc tgggttaatt acaactacga ctggatgttt aagccgggtg
    10081 ctatgaaaca ggtggcggaa tatgccgacg gtatcgggcc ggattaccat atgttgattg
    10141 aggaaacatc gcagccgggt aatatcaaac tcactggcat ggtgcaagat gctcagcaga
    10201 ataaactggt agtgcatcct tataccgtgc ggtcagataa actgcctgaa tacactactg
    10261 atgtgaatca gttatatgat gctctgtata acaaagcggg tgtaaatggg ctgtttactg
    10321 atttccctga taaggcagta aaattcctta ataaagagta atgctactgc aggatggcgt
    10381 tcatgcgcca tcctgtctca catcaaattt ttccattcac cacgagttgt tccgtacacg
    10441 tttgtagtaa aaacagtgga ctgccttcta ccggcttttc tttagccaac aacgaaatct
    10501 gtcgatagaa ggttggttcg tgaggaacgg aacacaattc acctgcaatg cttttcagcg
    10561 ttaactctgg ttgtaaggcg atgcctaatc cctgtcgaat aaacttcatc gctgtatccg
    10621 actgattaaa ttcacatttt atctactggg cgcactatta gtggtagtgg ctggtggaat
    10681 ctatgcgctg caaatgaagg gtaaaagcgg tgtcacgcta gtagtggcaa aagaaattca
    10741 ttaatacgac acgtttcatt aagattttcc tcaggtaacc acctatagtc attgtcgtta
    10801 ataaaaatga caggcgatag ggtatggcag tttcagcaaa gtacgacgaa tttaatcact
    10861 ggtgggcaac agagggcgac tgggttgaag agcctaacta tcgccgtaat ggtatgagcg
    10921 gggtgcaatg cgtcgagcgc aacggcaaaa agctgtatgt aaagcgcatg acgcatcatc
    10981 tgtttcattc cgtacgttat ccgttcggcc gaccaacgat tgtccgtgag gttgcggtaa
    11041 ttaaagaact ggaacgggcc ggtgtcatcg tgccgaagat cgtttttggt gaagcggtga
    11101 aaattgaggg tgaatggcga gcgttgctgg tgactgaaga tatggcgggg ttcatcagca
    11161 ttgctgactg gtatgcccag catgcagtat cgccttattc tgacgaagta cggcaagcca
    11221 tgttgaaagc agtggcgctg gcgtttaaga aaatgcatag cattaatcgt cagcatggct
    11281 gttgttatgt tcgccatatc tatgtgaaaa cagaaggtaa tgcagaagct ggttttcttg
    11341 acctggagaa aagtcgtcgc cgcttgcgtc gcgataaagc gattaatcat gacttccgcc
    11401 agctggaaaa atatctggag ccaatcccca aagcggactg ggagcaggtg aaagcgtatt
    11461 actacgccat gtaatcgttg taggccagat aagacgcata agcgttgtat ctggcactac
    11521 aaccaattca catctcgatt tcaatatcgc cttttgcccg gcaacaacag ggcaaaattt
    11581 cccccggctg aataaaggct aacggttcgg caatccagtc aacctgacct gcaaccagac
    11641 gggtgcgaca ggagccgcag taaccttcgc gacactgata ctcaaccgcc acattgtggg
    11701 attccagcgc cgccagaagg gaagggtgtt catcctggca cagcagttgt gtgccagtga
    11761 tgcgcagggt aacgcgggcc atcagagttg gaagttactc aaatcgtcgg tgtccacttc
    11821 cgcgtcaatc tgcccgacca gataagaact gacttccact tcctgcggag caacctgcac
    11881 gttatcagac accagccagg tgttgatcca tgggatcggg ttggagcgcg tctggaacgg
    11941 cagatccaaa ccgactgcct gcatacggat attggtgatg tattcaacgt actggcagag
    12001 aatgtcttta ttcagaccaa tcatcgaacc gtcgcggaac agataatccg cccagtcttt
    12061 ctcctgttga gctgcctgaa caaacaggtc atagcactcc tgcttacact cttcggcaat
    12121 ttccgccatc tcaggatcgt ccgcgccgct gcgcagcaga ttcagcatat gctgggtgcc
    12181 ggtcaggtgc agggcttcgt cgcgggcaat caggcgaata attttggcgt tgccttccat
    12241 caattcgcgt tctgcaaatg cgaaggaaca agcaaagctg acgtagaaac gaatcgcttc
    12301 cagcgcgtta acgctcatca ggcagagata cagttttttc ttcagctcgc gcaggctaac
    12361 ggtcacagtt ttaccgttaa cggtgtgggt accttcgccc agcagatgcc agtagctggt
    12421 catttcgatc agctcatcgt aatagctgga gatcccttcc gcacgtttct ggatctgctc
    12481 gttggtgacg atatcgtcaa acacaacaga cggatcgtta acgatattac gaatgatatg
    12541 agtataggaa cgggaatgaa tcgtttctga gaacgcccag gtttcgaccc aggtctccag
    12601 ttccgggatc gagatcagcg gcagcagtgc cacgttcggg ctacggccct gaatggaatc
    12661 cagcagcgtc tgatatttca ggttgctgat aaagatgtgt ttttcgtgtt caggcagtgc
    12721 ctggtaatcg atacggtcgc gagaaacgtc aacttcttcc ggacgccaga agaaagagag
    12781 ctgcttttcg atcagctttt cgaagatgtc atatttttgc tgatcgtagc gagccacgtt
    12841 gaccggctga ccaaagaaca tcggttcttt gagctgatca tttttcgtct gtgaaaaggt
    12901 ggtatatgcc atgagtgtgt cctgtggttt tattacccct caccctaacc ctctcccgca
    12961 aggggagagg ggacggtttg gtgcaaaggc tgggggatta tcagatctta catgcgccgc
    13021 tttcgcagcc atcgtcctgg attgacggca ccagatcgtc ctgcgcgtct tcagcgccgt
    13081 cacgggtgtt ctgataatac agcgttttga ccccgaattt gtaggcggtg agcaggtctt
    13141 tcagcaactg ctgcatcggc acttttcctg acgggaaacg tgacggatcg tagttggtgt
    13201 tagcagagat cgactgatcg ataaatttct gcatgatacc taccagttgc aggtagccat
    13261 cgttacccgg catttcccac agcagctcat aggcgtcgtg caggtgctcg tagtccggca
    13321 ccacctggcg caaaataccg tctttcgacg ctttgatgct gacgtaaccg cgcggcggtt
    13381 caataccgtt agtggcgtta gagatctgtg aagaagtctc tgacggcatc agggcagaaa
    13441 gcgtggagtt acgcaggccg tgcgttttga ttgattcacg caatgcttcc cagtcgtaat
    13501 gcagcggctc attagcgatg gtatccagat ctttcttata ggtatcgatc ggcaggatcc
    13561 ctttcgcgta agtggtttcg ttaaaccacg ggcacgcgcc ttgctctttt gccagctcgt
    13621 tagacgcttt cagcaggtaa tactgaatcg cttcgaaggt tttatgcgtc aggttgttgg
    13681 cgctgccgtc ggagtaacgc acaccgtgct tcgccaggta gtaagcgaag ttgatcacac
    13741 caatacccag cgtacgacga cccatcgctc cacgtttggc ggccgggatc gggtaatcct
    13801 gataatccag cagcgcgtca agtgcacgaa ccgccagaat tgccagctct tccagttcat
    13861 ccaggctatt aattgcgccc aggttgaaag cagacagcgt acacagcgcg atttcaccgt
    13921 tctcgtcgtt gacgtcgttc agcggtttgg tcggcagggc tatctccagg cacaggttag
    13981 actgacgcac tggcgcgatg gccggatcaa acgggctatg ggtattgcag tggtcaacgt
    14041 tctgaatata gatacgaccg gtagacgcac gttcctgcat catcagcgag aacagctcaa
    14101 cggctttcac acgctgcttg cggatgctgt cgtctttctc atatttggta tacaggcgtt
    14161 caaactcttc ctgatcggcg aagaatgcgt cgtacagccc cggtacgtcg gacgggctga
    14221 acagggtgat atcttcacct ttcagcaggc gggtatacat cagtttgttg atctgtaccc
    14281 cgtagtccat atggcgcacg cggttgcctt ccacaccacg gttgtttttc aacaccagca
    14341 ggctttccac ttccagatgc cacatcgggt agaacagcgt tgccgcaccg ccgcgcacgc
    14401 cgccctgaga gcaggatttc accgctgtct ggaaatgttt gtagaacgga atacagccgg
    14461 tatggaacgc ttcaccaccg cgaatcgggc tacccagcgc acgaatacgc ccggcgttga
    14521 tgccgatccc ggcacgctgg gaaacgtatt tcacaatcgc gctggaggta gcgttgatgg
    14581 aatccagact gtcaccgcac tcgatcagta cgcaggagct gaactgacga gtcggggtac
    14641 gcacgccgga catgattggc gtcggcagcg aaattttaaa tgtggaaacc gcgtcgtaaa
    14701 aacgcttcac atattgcagg cgcgtttcac gcgggtagtt cgagaacagg cacgcggcaa
    14761 ccagaatata aaggaactgg gcgctttcgt agatttcgcc ggtcacgcgg ttctgtacca
    14821 gatatttgcc ttccagctgc ttaacggcag cataagagaa ggtcatatca cggtcgtgat
    14881 cgataaacgt gtccatctgc ttgaactctt cttccgtgta gtcttccagc agatgattat
    14941 cgtatttgcc catctcgacc attttcacca cgtggtcgta cagcgcaggc ggctcaaact
    15001 ggccgtaggc ttttttacgc aggtggaaga tcgccaggcg cgcggcgaga tactgataat
    15061 ccggcgcatc acgggagatc aggtctgcgg ccgccttgat aatggtttcg tggatgtcag
    15121 aggtcttgat accgtcataa aactgaatgt gggagcgcag ctcgacctgg gaaatcgaaa
    15181 cgttatgcag tccttctgcc gcccaatcca gaacgcgatg gattttgtcg agattgatgc
    15241 gctctgtgct accgtcgcgc tttgtcacca gcagattctg attcatgtat gtcgtacctg
    15301 tttttggaat tctttccgca atagtttcat gcagactacc tgtagtacgg gaagctgctt
    15361 agaacactat atataggggg tatgtttggg attcactgca agatagtgtg aaaatggccc
    15421 tcttgcaagt gcataacttt gtggataact caggaaggaa aaagtggctt tcgcgcacct
    15481 taggtcagac aaggtgtccg ggaaagtcaa tgggaagaga aaaatttgtt aaaaataact
    15541 gttcggcgat ttcttgaacg gcagaatagg ttggataaat tgatatagat ggtttattaa
    15601 tctttttgct aatgattgca aaaattgatc taagcacgag tcatcacata atctgatgta
    15661 tcagttgtgg tcaatatata agcgaatgta agcgattgtt gatgtcgaaa tggttgtttt
    15721 ctattcggtt attgttaaat gcgaagccat tttgttaatt taccttatat tataagaaaa
    15781 ttattcatta ctttaattta gtttaaatat aactggtatc ccttaaggtt attaacttga
    15841 attatgacgc actgatatta ttcatcaaat aataacaaaa tagccattgc atcgggttgg
    15901 acaatttaca aaagaaagat aacccatatt gtataatgcg tttaaatatg ccgtcttata
    15961 taaaatgatg gtcagattaa tatgcggatt atctttctac gcaaggagta tttatctcta
    16021 ctcccgtcaa tgattgcatc tcttttctct gctaacggtg tcgcggcggc cattgattta
    16081 tgccagggat atgatatcaa agcgagttgt cacgccagca ggcaaagcct ttcaggcatt
    16141 acgcaggact ggagtgtcgc cgatgggcaa tggctggttt tttcggatat gaccaataat
    16201 gccagcggag gagccgtatt tttgcaacaa ggagcgcaat tttcactatt accagaaaat
    16261 gaaactggaa tgactctgtt tgccaataac accgttacag gagaatataa taacggcggg
    16321 gccatatttg ctaaagaaaa ctcaacgctg aatcttactg atgttatttt ttccggtaac
    16381 gtcgcaggcg gctatggtgg cgcaatctat tcttctggta ctaacgatac tggtgccgtc
    16441 gatttacgtg tcactaacgc catgtttcgc aataacatcg ctaatgatgg caaaggtggc
    16501 gcaatttata ccattaataa tgacgtttat ttaagtgatg ttatttttga taacaaccag
    16561 gcatatacat caacaagtta cagtgatggc gatggcgggg caatcgatgt taccgataat
    16621 aatagcgaca gcaagcatcc ttcaggttat acgataataa ataacaccgc ctttacaaat
    16681 aacactgccg aaggttatgg cggggcgata tataccaata gcgcgacggc tccctatctt
    16741 attgatattt ctgttgatga cagctacagc cagaacggcg gcgtgttagt ggatgagaac
    16801 aacagcgcag caggctatgg agatggtcct tccactgcgg cgggtggctt tatgtatctc
    16861 ggcttaagtg aagttacctt tgatattgcc gacggaaaaa cgctggttat tggcaataca
    16921 gagaatgacg gagctgttga ctctattgct ggtaccgggt tgatcaccaa aacaggttcc
    16981 ggcgatctgg tacttaatgc agataacaat gactttactg gtgagatgca gattgaaaac
    17041 ggtgaagtta ccctgggtcg cagcaactca ctgatgaatg tcggcgatac gcattgccag
    17101 gacgatccgc aagactgcta cggtctgacg atagggagta ttgataagta ccagaatcag
    17161 gcagagctaa acgttggctc cacccaacaa acctttgtgc actcattgac gggctttcag
    17221 aatggcactt taaatatcga tgctggtggc aatgttactg ttaatcaagg tagttttgct
    17281 ggcaccatcg aaggtgctgg tcagctcacc attgcgcaaa acggcagcta cgtgctgtcg
    17341 ggggcgcagt cgatggcgct aaccggcgat atagtcgttg atgatggtgc ggtgctttcg
    17401 ctggaaggcg acgcggcaga tcttaccgct ctccaggacg atccgcagtc gatcgtgtta
    17461 aacggcggtg tgctcgatct ctctgatttc tccacctggc agagtggcac atcatacaac
    17521 gatggccttg aagtcagcgg cagcagcgga acggttatcg gcagtcagga tgtggtagat
    17581 cttgcaggtg gaaacgatat gcatatcggc ggtgacggga aagatggcgt ctacgtggtg
    17641 gtcgatgcgg gcgacgggca ggtaagtctg gcaaacaata atagttattt gggcacaaca
    17701 caaatcgcct ccggtatgct ggaggtgagc gacaactcac agcttggaga tacccactat
    17761 aaccgccagg ttatctttac cgataagcaa caagaaagcg tgatggagat tactgccaat
    17821 gtcgatactc gctctacgac gactgagcat gggcgtgata ttgaaatgcg cgccgacggt
    17881 gaagtggcag ttgatgcggg ggtagacacg cagtggggcg cactgatggc tgacagcagc
    17941 gggcagcatc aggatgaggg tagcacattg actaaaacgg gggcgggtac gctggagctg
    18001 accgccagcg gtacaacgca gtcggcggta cgtgtcgaag aaggcaccct gaaaggcgat
    18061 gtcgcggata tccttcctta tgcttcgtca ctgtgggtcg gtgatggggc gacgttcgtt
    18121 actggcgcgg atcaggatat tcaatctatt gatgctactt ccagcggcac tatcgacatc
    18181 agcgatggta cggttttgcg cctgaccggg caggatactt ctgtcgccct taatgcctca
    18241 ctgtttaacg gcgatgggac gctggtgaat gccaccgatg gcgtgacgtt gacaggtgag
    18301 cttaatacca accttgaaac tgacagcctg acctatcttg ccgacgtgac ggttaatggc
    18361 aatctgacca atacgtccgg tgcggttagc ctgcaaaatg gcgtcgctgg cgatacgctg
    18421 acggtaaacg gtgattatac cggcggcggt acgctactgc tcgatagcga attaaacggc
    18481 gatgactcgg taagcgatca attggtgttg aacggtaata ctgctggcaa cacaactgtg
    18541 gtggttaact ccattacagg gattggtgag ccgacatcga caggcattaa agtggttgat
    18601 ttcgcagccg atcccacgca gtttcaaaac aatgcgaagt tcagtctggc aggcagcggc
    18661 tacgtcaata tgggagcata tgactacacg ctggtggaag ataacaacga ctggtatctg
    18721 cgatcgcaag aagtaacgcc accattgcca cctgatccag accctgaccc aacacccgat
    18781 cctgacccaa cacccgatcc aatccccgct taccagccgg tgctgaatgc caaagttggc
    18841 ggttatctca ataatctgcg ggcggcaaat caggcgttta tgatggagcg acgcgatcat
    18901 gcaggtggca atggtcagac gctgaattta cgtgttatcg gcggacgtta tcattacacg
    18961 gcggcagggc aactggctca gcatgaagac acttctacgg tgcagcttag cggtgacctg
    19021 tttagcgggc gctggggcga tgatggcgag tggatgcttg gggcggttgg tggctacagt
    19081 gataatcagg gcgagagccg ctcgaatatg accggaactc gcgccgataa ccagaaccac
    19141 ggttatgccg ttgggctgac ctcaagctgg tatcagcacg gtaatcagaa gcagggggcc
    19201 tggctggata gctggctgca atacgcgtgg tttaacaatg atgtttccga acaagacgat
    19261 ggcacagatc attaccactc gtcggggatt atcgcctcgc tggaagccgg gtatcagtgg
    19321 ttaccggggc gtggtgtggt gattgaaccg caggcgcagg tgatttatca gggcgtgcag
    19381 caggatgatt ttaccgccgc taaccatgcg cgcgtgtcac aatcgcaggg ggatgatatt
    19441 cagacgcggc tgggtttaca cagcgaatgg cgtaccgcgg ttggtgtcac accaacatta
    19501 gatctgaatt attatcacga tccccatgcg acggaaattg aagaagatgg cagcactatc
    19561 agtgacgatg cggtgaagcg acggggtgaa ataaaagtgg gagtcacggg caatatcagt
    19621 cagcgagtgt ccctgcgcgg cagcgtggcg tggcagaaag ggagtgatga ttttgcccag
    19681 acggcagggt ttttgtcgat gacggtgaaa tggtaatcac tgtctagtcg gcacggttgc
    19741 cggatgcggc gtaaacgcct tatccggcct acggggagca tttgtaggcc tgataagacg
    19801 cggcaagcgt cgcatcaggc attggctcgg ccaaacaaca acctcactta ttctgcgtgt
    19861 gcagcatata gttcacatcc acgccggggc cgagtttaaa agtattagtg agcgggttgt
    19921 aatgtagccc ggttatatgt cgctctttaa ggcttgtctg atctacccag ccaagcagtt
    19981 ctgccggttt aataaacttc ttcacatcat gcgtgccttt gggcaccatg cgcaaaatat
    20041 attccgcgcc aaccaccgcc atcagccatg acttgccgtt gcggttaagc gtcgagaaaa
    20101 agacatcgcc gcctggtttc accagttgcg cacaggctct gaccactgac tgcggatcgg
    20161 ggacgtgctc cagcatctcc atgcaggtca ccacatcata ctgcccggca tgttttgccg
    20221 cgtgctcttc cacggtttcc tgcacataat ccacctgaat gccgctttcc agcgcgtgca
    20281 gttttgccac ctgcaatggc tcaaagccca tatccagacc ggtcaccgtc gcgccttcgc
    20341 gcgccatact ctcggccaga atgccgccgc cacaaccgac atcgagcacc tttttgccaa
    20401 ataaaccgcc agcacgctcg gcaatatagc ccagacgcag cgggttaatg cggtgcaacg
    20461 gtttgaactc accttcaaga tcccaccagc gggaggcgac ggcttcaaat ttagcgatct
    20521 cttcgtggtc tacgttatgg ttttccggcg atttttcggc attcattggc acttctactc
    20581 cgtaattggc aagacaaacg agtatatcag gcattggatg tgaataaagc gtataggttt
    20641 acctcaaact gcgcggctgt gttataattt gcgacctttg aatccgggat acagtagagg
    20701 gatagcggtt agatgagcga ccttgcgaga gaaattacac cggtcaacat tgaggaagag
    20761 ctgaagagct cctatctgga ttatgcgatg tcggtcattg ttggccgtgc gctgccagat
    20821 gtccgagatg gcctgaagcc ggtacaccgt cgcgtacttt acgccatgaa cgtactaggc
    20881 aatgactgga acaaagccta taaaaaatct gcccgtgtcg ttggtgacgt aatcggtaaa
    20941 taccatcccc atggtgactc ggcggtttat gacacgatcg tccgtatggc gcagccattc
    21001 tcgctgcgtt acatgctggt agacggtcag ggtaacttcg gttccatcga cggcgactct
    21061 gcggcggcaa tgcgttatac ggaaatccgt ctggcgaaaa ttgcccatga actgatggcc
    21121 gatctcgaaa aagagacggt cgatttcgtt gataactatg acggcacgga aaaaattccc
    21181 gacgtcatgc caaccaaaat tcctaacctg ctggtgaacg gttcttccgg tatcgccgta
    21241 ggtatggcaa ccaacatccc gccgcacaac ctgacggaag tcatcaacgg ttgtctggcg
    21301 tatatcgatg atgaagacat cagcattgaa gggctgatgg aacacatccc ggggccggac
    21361 ttcccgacgg cggcaatcat taacggtcgt cgcggtattg aagaagctta ccgtaccggt
    21421 cgcggcaagg tgtatatccg cgctcgcgca gaagtggaag ttgacgccaa aaccggtcgt
    21481 gaaaccatta tcgtccacga aattccgtat caggtaaaca aagcgcgcct gatcgagaag
    21541 attgcggaac tggtaaaaga aaaacgcgtg gaaggcatca gcgcgctgcg tgacgagtct
    21601 gacaaagacg gtatgcgcat cgtgattgaa gtgaaacgcg atgcggtcgg tgaagttgtg
    21661 ctcaacaacc tctactccca gacccagttg caggtttctt tcggtatcaa catggtggca
    21721 ttgcaccatg gtcagccgaa gatcatgaac ctgaaagaca tcatcgcggc gtttgttcgt
    21781 caccgccgtg aagtggtgac ccgtcgtact attttcgaac tgcgtaaagc tcgcgatcgt
    21841 gctcatatcc ttgaagcatt agccgtggcg ctggcgaaca tcgacccgat catcgaactg
    21901 atccgtcatg cgccgacgcc tgcagaagcg aaaactgcgt tggttgctaa tccgtggcag
    21961 ctgggcaacg ttgccgcgat gctcgaacgt gctggcgacg atgctgcgcg tccggaatgg
    22021 ctggagccag agttcggcgt gcgtgatggt ctgtactacc tgaccgaaca gcaagctcag
    22081 gcgattctgg atctgcgttt gcagaaactg accggccttg agcacgaaaa actgctcgac
    22141 gaatacaaag agctgctgga tcagatcgcg gaactgttgc gtattcttgg tagcgccgat
    22201 cgtttgatgg aagtgatccg tgaagagctg gagctggttc gtgaacagtt cggtgacaaa
    22261 cgtcgtactg aaatcaccgc caacagcgca gacatcaacc tggaagatct gatcactcag
    22321 gaagatgtgg tagtgacgct ctctcaccag ggctacgtta agtatcagcc gctttctgaa
    22381 tacgaagcgc agcgtcgtgg cgggaaaggt aaatctgccg cacgtattaa agaagaagac
    22441 tttatcgacc gactgctggt ggcgaacact cacgaccata ttctgtgctt ctccagccgt
    22501 ggtcgcgtct attcgatgaa agtttatcag ttgccggaag ccactcgtgg cgcgcgcggt
    22561 cgtccgatcg tcaacctgct gccgctggag caggacgaac gtatcactgc gatcctgcca
    22621 gtgaccgagt ttgaagaagg cgtgaaagtc ttcatggcga ccgctaacgg taccgtgaag
    22681 aaaactgtcc tcaccgagtt caaccgtctg cgtaccgccg gtaaagtggc gatcaaactg
    22741 gttgacggcg atgagctgat cggcgttgac ctgaccagcg gcgaagacga agtaatgctg
    22801 ttctccgctg aaggtaaagt ggtgcgcttt aaagagtctt ctgtccgtgc gatgggctgc
    22861 aacaccaccg gtgttcgcgg tattcgctta ggtgaaggcg ataaagtcgt ctctctgatc
    22921 gtgcctcgtg gcgatggcgc aatcctcacc gcaacgcaaa acggttacgg taaacgtacc
    22981 gcagtggcgg aatacccaac caagtcgcgt gcgacgaaag gggttatctc cattaaggtt
    23041 actgaacgta atggtttagt ggttggcgcg gtacaggtag atgactgcga ccagatcatg
    23101 atgatcaccg atgccggtac gctggtacgt actcgcgttt cggaaatcag catcgtaggc
    23161 cgtaacaccc agggcgtgat cctcatccgt actgcggaag atgaaaacgt agtgggtctg
    23221 caacgtgttg ctgaaccggt tgacgaggaa gatctggata ccatcgatgg cagtgccgcg
    23281 gaaggggacg atgaaatcgc tccggaagtg gacgttgacg acgagccaga agaagaataa
    23341 ttttacttct tcatgccaaa agggagctat ctcccttgtt tgaattgaaa agtccaggct
    23401 gcaaagtctg ggcttttgtc gtattagggc acggtaaagt ttggctgtgc ccgtaaaaaa
    23461 tggctggcta tacacaagga atgtggcaat gagtggtgaa aaaaaggcga aaggctggcg
    23521 gttctatggt cttgtaggtt ttggcgcaat agcactgctt tccgctggcg tctgggcgtt
    23581 gcaatatgct ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct
    23641 gcaaatcgat ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc
    23701 caaagatctc ctcaccattc cgtttctcca cgatgttctg agcgaagatt tcgttttcta
    23761 ttatcagaat catgccgatc gtctgggcat tgaaggcagc attcgtcgta ttgtctatga
    23821 acacgatctc acgctgaaag ataagctctt ttcgtcactc ttagatcagc ccgcgcaggc
    23881 agcgctgtgg cacgataaac aaggccatct ttcacattat atggtgctga tccagcgcag
    23941 tggtttaagc aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag
    24001 caaaacggaa atcagtagca tcaagataaa tagtgaaact gttcctgttt atcagttgcg
    24061 ctataacggc aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc
    24121 cagcacggat atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg
    24181 tgatttgttg agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc
    24241 tgaaaaaacg ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta
    24301 ccagcggtta atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca
    24361 cagttttgtg gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc
    24421 ggtctggaac agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg
    24481 tattgccgaa gagatgcttt cgcacatcag tcaggaaaac gacaagttga atggggcgtt
    24541 agacggtgcc gcggggctgt gctggtatgc agactcaaaa ttgcaaaccc cgctgtttgt
    24601 cggtcagttt gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa
    24661 tattggtgcg cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca
    24721 gggcgaagcg caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc
    24781 gcaggcggcg caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat
    24841 gcaaaacaaa acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac
    24901 actgaataaa aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct
    24961 ctatatcaat ccacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc
    25021 gaagaatctc gaaccggttt tttataacgc cgcacaaact ttattaatgc cgaagctgga
    25081 cgctttatct caacaaccgc gttatgtcat gaagctggcc cagatggaac ccggtgccgc
    25141 ctggcagtgg ctacccataa cctggcagcc gctatgaggc acgggctgct ggcgctgatt
    25201 tgctggctgt gttgtgttgt tgcccatagc gaaatgctga atgtcgaaca atccgggctg
    25261 tttcgcgcct ggtttgtacg cattgcacaa gaacagctcc gccaggggcc aagtccacgc
    25321 tggtatcagc aggattgtgc gggcctggtg cgatttgcgg cgaacgagac gctgaaagtt
    25381 cacgatagta aatggctaaa aagtaacggt ttctctagcc agtatttgcc gccagagatg
    25441 acgctaacac ctggacagcg tcaactggcg caaaactgga atcaggggaa cgggaaaacc
    25501 ggcccctacg tgaccgcgat taatttgatt cagtacaaca gccagtttat tggccaggac
    25561 ataaaccagg cgctgcctgg cgatatgatt tttttcgatc agggcgatgc ccagcactta
    25621 atggtctgga tggggcgtta cgtcatctac cacaccggaa gcgccacgaa aactgacaac
    25681 ggaatgcgcg cagtcagtct gcaacaactt atgacatgga aggacacccg atggataccc
    25741 aacgattcca atcccaattt cattggcatt tatcgtttaa attttctggc gcgatagcag
    25801 cgtgcttgtc cctgtctctt gtgggaacag ggcttgctaa tgctgatgat tcgcttcctt
    25861 ccagtaacta tgcgccgccc gccgggggaa cattcttttt gcttgctgac agcagtttta
    25921 gcagcagtga agaggcgaaa gtgcgactgg aagcgccggg gcgtgattat cggcgctatc
    25981 agatggaaga gtacggcggc gtggacgttc gcctgtatcg tattcctgac ccgatggcat
    26041 ttttgcgcca gcagaaaaac ctgcatcgca ttgtggtgca accgcaatat ctgggcgacg
    26101 ggctgaacaa tacgctgacc tggctgtggg ataactggta cggcaaatct cgccgcgtaa
    26161 tgcagcgtac tttctcttct cagtcacggc agaatgtgac tcaggcatta cccgaattac
    26221 atctcggcaa tgccattatt aaaccttccc gttatgtaca gaacaaccag ttttccccgc
    26281 tgaaaaaata tcccttggtg gaacagttcc gttatccact atggcaggct aaaccggtcg
    26341 agccgcagca aggggtaaaa ctggaaggcg catccagcaa tttcatctcg ccgcagccgg
    26401 gtaacattta tattcctctc ggccaacaag agccgggact gtacctcatc gaggcgatgg
    26461 ttggtgggta tcgggcgacg acggtggtgt ttgtttccga taccgtggcg cttagcaaag
    26521 tgtcaggcaa cgagcttctg gtgtggaccg cgggtaaaaa acagggtgaa gcgaagcccg
    26581 gctcagagat cttgtggact gacggtcttg gcgtgatgac ccgcggtgtg accgatgaca
    26641 gcggtacctt gcagttacaa catatatcgc cagaacgttc atacattctg ggtaaggatg
    26701 ctgaaggcgg tgtttttgtc tccgagaacc tcttctacga aagcgaaatc tacaacaccc
    26761 gcttgtatat ttttaccgat cgcccgctat atcgcgcagg cgatcgtgtc gatgttaaag
    26821 tgatgggccg cgagttccac gatccgttgc attcatcccc catcgtcagc gccccggcga
    26881 agctttcggt gctggacgct aacggcagtc tgttgcaaac cgtcgatgtc acgctggatg
    26941 cgcgcaatgg cgggcaggga agtttccgcc tgccagaaaa tgccgttgcc ggaggttatg
    27001 agttacgtct tgcttaccgc aatcaggtct atagcagcag ttttcgcgtg gcaaactaca
    27061 tcaagccaca tttcgagatt ggtttagctc tcgacaaaaa agagttcaaa actggcgaag
    27121 cggtcagcgg caaactgcaa ctgctctacc cggatggcga gccggtaaaa aatgcccgcg
    27181 tgcagttaag tttgcgcgct cagcaattat caatggtcgg taacgatttg cgttatgccg
    27241 gacgtttccc cgtgtcgctg gaaggcagcg aaacggtgtc cgacgccagc ggtcatgtgg
    27301 cgttaaatct ccccgccgcc gataaaccga gccgttattt gttaaccgtc tccgccagtg
    27361 acggcgcggc gtatcgtgtc accaccacca aagagatcct cattgaacgc ggtctggcgc
    27421 attactcatt aagtactgcc gcacaataca gtaatagcgg cgagtcggtt gtgttccgtt
    27481 atgccgcgct ggaatcttca aaacaggttc ctgttacgta tgaatggttg cgtctcgaag
    27541 accgcacgag ccatagcgga gagctaccgt caggcggcaa atcctttacc gtcaatttcg
    27601 ctaaacctgg caactacaat ctgacattac gcgataaaga cggcttaatt ctcgctgggt
    27661 taagtcatgc cgtcagcggt aagggcagca cggcgcatac tggtacggta gatatcgtgg
    27721 cggataaaac gctgtaccag ccaggcgaaa ctgcgaagat gctgattacc tttccggagc
    27781 caattgatga agcattattg acgctggaac gcgatcgcgt ggaacagcag tcgctgcttt
    27841 cgcatccggc aaactggctg acgttacaac gtttaaacga tacccagtat gaagcccggg
    27901 ttccagtgag caattccttt gcgcctaaca tcactttttc ggtgctgtat acccgtaatg
    27961 gtcagtacag ttttcagaac gccgggatca aagttgccgt tcctcagctt gatatccggg
    28021 tgaaaacgga caaaacccat taccagcctg gtgaactggt caatgtcgaa ttaacctcgt
    28081 cgctgaaagg taaacctgtt tctgcgcagc taacggtagg cgtggtcgat gaaatgatct
    28141 acgcgctgca accagaaatc gcgccgaata tcggcaaatt tttctatccg ctggggcgta
    28201 acaatgtgcg taccagctcc agtctgtcgt ttatcagcta cgaccaggcg ctctccagcg
    28261 agccggttgc gcctggcgca actaaccgca gcgagcggcg agtaaaaatg cttgaacgtc
    28321 cacggcgtga agaggtggat accgcggcat ggatgccgtc actcacaacc gataaacaag
    28381 gcaaagcgta tttcacgttc ctgatgcctg attcgttaac ccgctggcgt atcaccgcgc
    28441 gtgggatgaa cggcgacggg ctggtcgggc aggggcgtgc ttatctgcgt tcggaaaaaa
    28501 atctctacat gaagtggagt atgccaacgg tgtatcgcgt gggcgacaaa ccggcggcag
    28561 gactgtttat cttcagtcag caggataacg aaccggtggc gctggtgact aaatttgcag
    28621 gcgctgagat gcgccagacg ctgacgctgc acaaaggggc gaattatatt tcgctggcgc
    28681 agaacattca gcaatctggc ttgttaagcg cagaactgca acaaaatggg caagtgcagg
    28741 acagcattag cacaaaactg tcttttgtgg ataacagctg gcccgttgaa cagcagaaaa
    28801 atgtcatgct cggcggtggc gagaacgcgc tgatgttgcc cgagcaggct agcaatatcc
    28861 ggctacaaag tagcgaaacg ccgcaggaga ttttccgcaa caatcttgat gcgttagtcg
    28921 atggaccatg gggtggcgtg atcaacaccg gtagccgtct gatcccgctc agtctcgcct
    28981 ggcgttcgct tgccgatcat caaagtgccg ccgctaacga cattcgtcag atgattcagg
    29041 ataaccgtct gcggctgatg caactggcgg ggcccggagc gcgctttacc tggtggggtg
    29101 aagatggcaa tggtgacgcc ttccttacgg catgggcatg gtacgccgac tggcaggcca
    29161 gccaggcgct cggcgtaacg caacaaccgg aatactggca gcatatgctc gacagttatg
    29221 ccgagcaggc ggataacatg ccgttattgc atcgggcgct ggtgctggcg tgggcacagg
    29281 agatgaatct gccgtgcaaa acgttgttga aagggttgga tgaagctatc gcccggcgcg
    29341 gaactaaaga tgaagatttc tctgaggaag acatccgcga tatcaatgac agcctgatcc
    29401 tcgatacacc ggaatctcca ctgggagatg cggtggcaaa cgtcttaacc atgacgttgc
    29461 tgaaaaaagc gcagttgaag tccacggtga tgccacaggt tcagcaatat gcgtgggata
    29521 aagcggcaaa cagcaatcag ccgctggcgc acacggttgt gctgctcaat agcggtggtg
    29581 acgctaccca gacggccgct attttaagtg gtttgaccgc tgagcaatcc actattgagc
    29641 gcgcgctggc catgaactgg ctggcgaaat atatggcgac aatgcctcca gttgttttgc
    29701 ctgcgcctgc gggcgcatgg gctaaacata agttaactgg agggggcgaa tactggcgtt
    29761 gggttggtca gggtgtgccg gacattctct cttttggtga cgaattatcg ccgcaaaatg
    29821 tgcaggtccg ctggcgtgaa ccggcaaaaa cggctcaaca aagtaacatt ccggtgaccg
    29881 ttgaacgcca gttgtatcgg cttatccccg gtgaagaaga gatgagcttt actctgcagc
    29941 cggtgaccag caatgagatt gacagcgatg cgctgtatct cgatgaaatt acgcttacca
    30001 gcgagcagga tgcagttctg cgctatggtc aggtggaagt accgctcccg ccgggagccg
    30061 acgttgagcg cacaacatgg ggcatttcag tcaataaacc caacgccgcg aaacagcagg
    30121 ggcaattgct ggaaaaagcg cgtaatgaaa tgggcgaact ggcctatatg gtgccggtga
    30181 aagaactgac gggaacggtc actttccgcc atttgctgcg cttctcgcaa aaagggcaat
    30241 tcgttctgcc tcctgctcgt tatgtgcgtt cctatgcacc tgcgcagcaa agtgttgcgg
    30301 cagggagtga atggaccggg atgcaggtga aataagtgaa ctggcgcaga atcgtctggc
    30361 tattggcgtt ggttactctg ccaacgcttg cggaggagac gccgttacaa ctggtgctgc
    30421 gcggtgcgca gcacgatcaa ctgtatcagc tgtcgtcatc aggcgtgacc aaagtctcag
    30481 cattacctga ctcgctcacc acgccgttag ggagtttgtg gaaactatat gtttacgcct
    30541 ggctggagga tacccatcaa ccggagcagc cctaccagtg tcgcggaaac tccccggaag
    30601 aggtttattg ttgtcaggct ggggagagca taacgcgcga taccgcgctg gtacgttcct
    30661 gtgggctcta ttttgcgccg cagcggttgc atattggcgc agatgtgtgg gggcaatact
    30721 ggcaacagcg tcaggcacct gcgtggttag cgtctcttac aacgctgaag ccagaaacct
    30781 cggtaacggt taaatcgctg ctcgattcat tagcgacgtt acctgcgcaa aacaaagctc
    30841 aggaagtctt actggatgtg gtgttggacg aggcgaaaat cggtgtcgca tccatgctcg
    30901 gtagtcgggt acgggtgaaa acctggagct ggtttgccga tgataaacag gaaatccgcc
    30961 agggtggttt tgctggctgg ttaaccgatg gtacaccgct gtgggttact ggcagtggca
    31021 ccagcaaaac ggtactaacc cgttatgcga cggtgcttaa tcgggttttg ccagtgccga
    31081 cgcaggttgc cagtgggcaa tgtgtagagg tggagctgtt tgcccgctat ccgctgaaga
    31141 aaatcacagc ggaaaagagt acgacggcag taaaacccgg cgtacttaat gggcgctatc
    31201 gtgtgacgtt tactaatggt aaccacatta cgtttgtcag ccacggtgag acgaccttac
    31261 tgagcgaaaa gggcaaactg aaattgcagt cgcatctcga tcgtgaagag tacgttgcca
    31321 gagttctgga tcgcgaggcg aaaagtacgc cccctgaggc agcgaaagcc atgaccgtgg
    31381 cgattcgcac ttttttacag caaaacgcca atcgggaagg cgattgcctg accatccccg
    31441 acagcagcgc cacgcagcgt gtttctgctt cgccagcgac cacgggggcg cgaacgatga
    31501 ccgcctggac gcaggatctc atctatgcag gcgatccggt tcattatcat ggcagtcgag
    31561 ctaccgaagg gacactttcc tggcggcaag ccacggccca ggcggggcag ggagagcgtt
    31621 acgaccagat actcgcgttt gcttatcccg acaacagcct tagccgctgg ggagcgccac
    31681 gctcaacctg ccagttatta cccaaagcga aagcctggct ggcgaaaaaa atgccgcagt
    31741 ggcggcgtat attacaaggc gagacggggt acaacgaacc agacgtgttt gcggtctgtc
    31801 gtttagtctc aggtttcccc tataccgatc ggcagcagaa acggctgttt attcgtaact
    31861 tctttacgct tcaggatcgg ctggatttaa cccatgagta tctgcacctg gcctttgatg
    31921 gttatcccac cgggcttgat gagaactata tcgaaacgct gacccgacaa ttattaatgg
    31981 actaatgcta tgcgaaagat ttttcttccg ttgttactgg tggcactttc gcctgtcgct
    32041 cacagtgaag gtgtgcagga agtcgagatt gacgcaccgc tctctggctg gcatccggca
    32101 gagggcgaag atgccagttt ttcacaatcg atcaattacc cggcgtcgtc cgtcaacatg
    32161 gcagatgatc aaaatatctc agcgcagatc cgcggcaaaa taaagaatta tgctgcggcg
    32221 ggtaaagttc agcagggccg gctggtggtc aacggtgcca gtatgccgca gagaattgaa
    32281 tccgatggtt catttgcacg cccttatatt ttcactgaag gcagcaacag cgtgcaggtc
    32341 atcagcccgg atgggcaaag ccggcaaaaa atgcagtttt actcaacgcc gggtacagga
    32401 acgattcgtg cacgtttacg gctggttctc tcgtgggata cggacaatac cgatctcgat
    32461 ctccatgttg tcacgcccga tggcgaacac gcctggtacg gtaacaccgt gctgaaaaac
    32521 agtggtgcac tggatatgga tgtcacgacg gggtacggac ccgagatttt cgccatgcca
    32581 gcaccagttc acgggcgcta tcaggtgtat atcaactact atggtgggcg cagcgaaacg
    32641 gaattgacga ccgcccaact gacgctgatc accgatgaag gatcggtcaa tgagaaacag
    32701 gaaacattta ttgtaccgat gcgtaatgct ggcgagctga cgctggtgaa aagttttgac
    32761 tggtgatcca tcccttcaca caacctggct ctctcttttt tcagataaga ctgctgggcg
    32821 atctgccgac gccaggatat aaccattaac aagcgtcggc agattggtca tttcttattt
    32881 ttcgtactga gaactgtcca tctactccgg ctttcctggt taaggcaatg gtttctaagc
    32941 gatacgcatt attgcaatgg tgacagaaca ttgcggtaac acgcttttac cgctacctta
    33001 accacactcc atcggtcacc tgaggcggag cttcgcccct ttgaaatacc ttgcttcttt
    33061 tcgtacaacc ctgaaagcct cgcgctacat gttcagagca ttggcgttag tgctctggct
    33121 gttgattgct ttttcatccg ttttttacat cgttaatgcg ttacatcagc gagaatcgga
    33181 aattcgtcag gaatttaatc tgagttccga tcaggctcag cgctttattc aacgcacctc
    33241 tgatgtgatg aaagagctga agtacatcgc cgaaaatcgc ttatcggcag aaaacggtgt
    33301 gctttccccg cgtggacgag aaacgcaggc ggatgtgcct gcgtttgaac cgctgtttgc
    33361 cgactccgat tgttccgcaa tgagtaacac ctggcgaggt tctctggagt cattggcgtg
    33421 gtttatgcgc tactggcgcg ataatttttc tgcggcttac gatctcaacc gggtattttt
    33481 aatcggcagt gataacctct gcatggccaa tttcggtctg cgtgatatgc cagtggaacg
    33541 cgataccgcg ttgaaagctt tgcatgaacg catcaataaa tatcgaaatg caccacaaga
    33601 tgatagcggc agtaacctct actggatcag cgaaggtccg cgccctggcg tcgggtattt
    33661 ttacgcgttg acgccagttt atctggcgaa ccggttgcag gcgcttttgg gtgtcgagca
    33721 gaccatccgg atggagaact ttttcttacc gggtacgttg ccgatggggg ttaccattct
    33781 tgatgaaaat ggtcataccc tgatttcgct taccggacca gaaagtaaaa ttaagggcga
    33841 tcctcgctgg atgcaggaac gctcctggtt tggctatacg gaagggttcc gggagctggt
    33901 gctgaagaaa aatctgccac cctcatcgct aagcatcgtg tattcggtgc cggttgataa
    33961 ggtgctggaa cgcattcgca tgttgatcct taacgcaatt ttgctgaatg tgcttgccgg
    34021 agctgcattg tttactctcg cgcggatgta cgagcgacgt attttcattc cggcggaaag
    34081 cgacgccctg cgactggaag aacatgagca gttcaatcgc aagattgtcg cctccgcgcc
    34141 agtgggtatc tgtattttgc gtaccgctga tggcgtcaat attttaagta acgaactggc
    34201 gcatacctat ctcaatatgc ttacgcatga ggaccgccaa cgactgacgc aaattatctg
    34261 tgggcagcag gtcaattttg ttgatgtcct gaccagcaac aataccaatc tgcaaatcag
    34321 cttcgtccat tcgcgctatc gtaatgaaaa cgtggccatt tgtgtgctgg tggatgtttc
    34381 ttcgcgcgtg aagatggaag agtcgttgca ggagatggca caagcagcgg aacaggcgag
    34441 ccagtcaaaa tcgatgttcc ttgccaccgt cagtcatgag ctgcgaacgc cgctgtatgg
    34501 cattatcggt aacctggatc tgttgcaaac caaagagtta ccgaaaggcg tcgatcggct
    34561 ggtgacggca atgaacaact cttccagcct gttgttgaaa attatcagcg atattctcga
    34621 tttctcgaag attgaatcgg aacagttgaa gatcgaaccg cgtgagtttt caccgcgtga
    34681 agtgatgaac cacatcaccg ccaactattt accgctggtg gtacgcaagc agttaggctt
    34741 gtactgcttt attgaaccgg atgtgccagt ggccttaaat ggcgacccga tgcgtttaca
    34801 gcaggtcatc tccaacctgt tgagtaacgc cataaaattc accgataccg gctgtatagt
    34861 tttgcatgtc cgcgctgatg gcgattatct ctctatccgt gttcgcgata ccggcgtggg
    34921 gattccggcg aaagaagtgg tgcgcttgtt tgatcccttc ttccaggtcg gaacgggcgt
    34981 acagcgaaac ttccagggga ccggtctggg tctggcgatt tgtgaaaaac tgatcagcat
    35041 gatggacggc gatatctcgg tagattcaga accgggaatg ggcagccagt ttaccgtgcg
    35101 tattccgttg tacggcgctc agtacccgca gaaaaaaggc gtggaagggt tgagtggtaa
    35161 acgctgctgg ctggcggtcc gcaatgcgtc gctctgtcag ttcctggaaa ccagtttgca
    35221 gcgcagcggc atcgtcgtta caacatacga agggcaggaa ccgactcccg aagatgtgtt
    35281 aattactgac gaggtagtga gtaaaaaatg gcagggtaga gcggtagtga ccttctgtcg
    35341 tcgccatatt ggtattccgc tggagaaagc gccaggggag tgggtacaca gtgtggctgc
    35401 cccgcatgag ctaccggcat tgttggcgcg tatttatttg atcgagatgg agagcgacga
    35461 tcctgctaac gctctgccgt cgacggacaa agcggtcagc gataatgacg atatgatgat
    35521 tctggtcgtg gatgatcatc cgattaaccg acgtctgctg gcagatcagt tgggatcgtt
    35581 gggctatcaa tgtaaaaccg cgaatgatgg tgtcgatgcg cttaatgtac ttagcaagaa
    35641 tcatattgat atcgtgctta gcgacgtcaa catgccaaat atggatggtt accgcttgac
    35701 gcaacgcatt cgtcagttgg gactgacgtt gccggtaatc ggagtaactg ctaatgcgtt
    35761 ggctgaagag aagcagcggt gtctggagtc cggtatggac agctgcctgt cgaagccggt
    35821 aacgctggat gtgataaaac agacgctgac ggtatatgcc gagagggtca ggaaatcgcg
    35881 ggaatcgtag gacggataag gtgtttatgt cgcatctggc attcagtgca aatgccagat
    35941 gcgacgctga cgcgtcttat ctggcctact ttaatgtaat ctgttgaaat aatgggaatc
    36001 gtaggccgga taaggcgttt acgccgcatc cggcattcag tgcaaatgcc agataagaca
    36061 ctaacgcgtc ttatctggcc tacaggtgat tagtctttat ctgccggact taacgtcaca
    36121 gaagagagat aattcagcag ggcgatatcg ttctcgacac ccagcttcat catcgcagat
    36181 ttcttctggc tactgatggt tttaatactg cggttcagct ttttagcgat ctcggtcacc
    36241 aggaagcctt ccgcaaacag gcgcagaact tcactctctt ttggcgagag acgcttgtca
    36301 ccgtaaccac cagcactgat tttttccaac aggcgagaaa cgctttccgg ggtaaatttc
    36361 ttccctttct gcagcgcggc gagagctttc ggcagatcgg tcggtgcacc ttgtttcagc
    36421 acgatccctt cgatatccag atccaatacc gcactaagaa tcgccgggtt gttgttcatg
    36481 gtcagaacaa taatcgacag gcttgggaaa tggcgcttga tgtacttgat taaggtaatg
    36541 ccatcgccgt acttatcgcc aggcatggag agatcggtaa tcaacacatg cgcatccagt
    36601 ttcggcaggt tgttgatcag tgctgtagag tcttcaaatt cgccgacaac attcacccac
    36661 tcaatttgct caagtgattt gcgaataccg aacaagacta tcggatggtc atcggcaata
    36721 attacgttca tattgttcat gtacttgtac ctatgtatta actatttgat tttaaaaata
    36781 tattctatgc tttgtttagt cgttggggca cctgtgggac ataagtacat agctttctgt
    36841 ttagtacttc tacctgggac tggttattgt ctttcatcca tgcaccgtaa acattgtaaa
    36901 ccatctgggc attcgcatgt cccatctggt tggctatgaa gtttggattt gctccggcag
    36961 ttaaagccca gcaagcataa gtatgccgag actgatacgc ttttctatgg cgaatttttg
    37021 cccgttttaa tgcagattcc catgttctgt tgatggatgt tacggcataa tgaactcctg
    37081 agttacctgt gcgatctaat atctgaggat tgaaaacaaa gctgcatgag tgagtgcttg
    37141 tccggccata ctctcgcagc tttacttcta tctgatattg tttaccaagc ctggttaatg
    37201 tcgcctgatt ttttaatacg tcaattgctg gctgtatgag ttgtattacc ctgtttgttc
    37261 ctgcatctgt tttgggtagt gtaaattcct ttgtctgagt gtaattcctt ttgacggtca
    37321 aggtacccgc ttccagatcg atatcctccc atgccagccc acacagttcg ccatgccgca
    37381 tcccggtata tacagccagc gaccagaaat ttttagtttg ctggtggtgg cacgcatcaa
    37441 tcagtctggc aaactcatct tttgttagtg gatcgggagg tgttttcgct cttttcagtg
    37501 atggaagctc actgaaaggg tttttgccta tatacccatt gtttgccgca aaacggaaca
    37561 tcccggagat aattgtcata tagctattta cagtaggtac actacgacct ttaataggct
    37621 tcctctgatt ttttcctggc aactgatatc ccgttaataa ttctttacga aaaatgagaa
    37681 catcttcctg tgtaattgat gatgccagtt tgttccctcc aaggcagtca aggcaatttc
    37741 ggatagctga tttgtatctg accatggcat ttgctgttat ttccattttc ttaagctcaa
    37801 gccatttttc cgaaagtgcc ttaatggtta tctctttttt tcccagacca aaatgtttca
    37861 ggttagggga attagggaac tgcgcggcgt agtcgaagct ccccattctg attgcaaaac
    37921 aaacggaagt gcgaagttca cctgcgatct tccggttttt ggcggtgtca ggaacaccga
    37981 ggttttctct gacacgtttg ccgttatagt gaaaccatat gcggagtgat ccgccatggt
    38041 tttcaacgcc tgtcgggtat gatgcgttac tcattaaacc tcccagacgt ccaggagcat
    38101 taacaggtta accggaactt gcatttttgg cacctggttg tttctggttt tcgatccagc
    38161 gcataatttc ctcaatgttg tatacacatt cactgaaagg cccgggctct ccttctgctg
    38221 cgtaatgacg gtattctttc cccagtaacc aggatttttt tcttgctctg gcaatggttc
    38281 ctgatttcag tccagttgcg gcaataaggg ttctttctgt gcaccatttg ctgggcgtta
    38341 tctgatagat gattgtttcc attcctgact cacactatct tcaggccacg gcagtggcac
    38401 cacacgtcaa acattcgctt tacaacttca cggcagtaga agccgtgagc atctctggtt
    38461 aaatcgtatc ggtacccgta tctgagtcgt atccatattt caaactcgcg attcacgact
    38521 tattctcctg ctgtctttat gccagttcat tcggttgcgc tggggccctg gccttccgtg
    38581 gcctgtattt gcggcgtttt ctggcttcaa tgcgtatctt tgtcaccctg actggctttg
    38641 gttcgtgccg gaacactttg tttctgatat tacgccgtgt attcatcatc cagataaaac
    38701 gctgggtatg gtcgcatccg tcatcaacgg taattactgt gcggaccagt tcatcgttaa
    38761 tatcaaccat ttcacccatt cactgagccc tcatacgcga atttcgtggc ttccatcagt
    38821 tgcgtccata cccattcaag aaattgtgcg ttttctcctg agcggtcata ttcatcgacg
    38881 ctgcggggct tgatggtcgc cagtagtggc acaatccggt acttcatctg ttcggttaac
    38941 ttgtccggta caagtcgcca gccgttatat ttcgatggaa catggtggta ttcagccagg
    39001 aagcgtcgta tatcgttttt catttcctcg catgagttct gatcatgaag tgcagcggaa
    39061 agccagcgtc cgatatattc agggattcgc acatcaagta ctttttctgc cgtcgtcagc
    39121 attgcctgtc ggcaggcgtt ccagccgttg acatagtgac gtgttgcatc ccatgaaacc
    39181 tgcacatcag ggtaagcctc ctgttctgtt ctcactggcg gtatctgagg aacagtgaat
    39241 agtggactga cgatatattt atccagagag cctccccgct cttctgcaag actttcccac
    39301 gatgtcattg tggggaccct gtaacctgct tttttgtgcg tatacatcca tgccactctg
    39361 ccgttatcat ctgcggcggc aatgcgctgc caggcaggat aaagcggggt gtatgtttct
    39421 ctgtcattac gggcgtcagg catggcatca atactgcaaa cttcccccgt aaggttattc
    39481 agccaggcct tcgggctaat ctctgacgtt acgatttcat ccagtttttt ctccagattt
    39541 tctatgtgac tgattagcgc aaggataact ggcggagtgg caagggagtg aaatgcttcc
    39601 agatcacatc ccttgtcgcc atatacggta ttttgtgcgg cgtcacgcag tgctttcaga
    39661 tcagcttctt tcatcgttca gactcccttg ttgctgtttt gtaggcgcgc aggatgtcgc
    39721 gtgtttttcc tgataatgac gacttaacca ggaaaaagcc gctccggctt tctgtgatgt
    39781 ccggtgtgca cagaagtacc gcatcaacga cacgattatg ttttcgcaat gtcaggcggg
    39841 aactggtgat aatcagtttt gcctgttcgc cgtaatcggt gaatgaaata atcatctgat
    39901 ggcgatctcc gttatgaaag tgttcctgct ggttcaatgc tgataatttc tgctgcaata
    39961 agcatacctg ccagccatgc ttcgccgagc atgtcctcat ccgtacatgc aagatcaccg
    40021 atctgtattg tggccataat gttagtttca ccggtatatt catcctgtgc ttcttcatac
    40081 ggcaggtctt cataaaggtt ttcaattgcg caggttatta catccagtcc cgtcagattg
    40141 ccgacaacgt ccacttcaaa tgtttcgcgg tactcccatt gcccgagtgt cagacgaacc
    40201 gtctgtcttg ccatgcggcc gcataatgtc atgttcgggt cgtagttcat gattgccgca
    40261 gtagtgctat tcatgttctg cctccggtgt gtaaatagct ttgtcgtgtt taaattcacc
    40321 gttccagttt ttcttcatag ggagttcacc gttcatgtaa aactggtata aacggtggca
    40381 tcctttttcc agcaatacgg gggtgaattt cgtgaatgtt gctttcccgt gtggagtgat
    40441 ctgtactgct tcttcggtca ggtatctgtc acgggcataa gaggccgtgc gccagcgggg
    40501 tgttttctcc ggatcgcgtt gttcgttgaa tacccatttt cggccagaca gccaccacat
    40561 tatctggctg gtatttacgc cgttcagcgc acggcagaac tggggaacag tcatgccttt
    40621 agagaaatgt ttttccagac tgtctacggt tgcagagagc gttttatttt ccagggccat
    40681 agcttcagcc cgttcttcgg cctcaataac catcaacgcg agttccttgc gagtgagtac
    40741 gcgctgaaca tcgatgtttt cccgctgagt gaagtagaac tccacaaggt cttcgtgata
    40801 gttccacgcc tgatccgttt ccagcaattt tgcgtggttg gctgcgccgc gttcggtcca
    40861 gaggatgagg gatcgaacat tgctggcaat tttcacagag tagctttgag atactctgtg
    40921 cttaagttca cgtagttcat cgccttccag caaaaaataa tgctttccaa caacgaatcg
    40981 ttcttcgttt cgggaatgat tctggcgaat acggattggt gttgctccat agcctgccgc
    41041 cagttgttca gtggttataa cgcgttgacc gcgatattcg ataatttgca aatcacgggc
    41101 tgccaccggc acaagttcag ttgatttttt catcattaca cctcacgaaa agctgattag
    41161 tggaacactc tggtttccgg ttctgagttg tcagtggctt cactgataag cccatctaga
    41221 atcatgtgca tcgctctgta accttccatt gtgatgccgg ttttttcgcc gtcgcccaga
    41281 gcgccggtga atgctttgta cataagaatg cttttgagca gccctgattc cgctccgtaa
    41341 gtttctatga gtgcccattc catatagttc tgcatagcga gacgtaatgg tttcaggtag
    41401 attgtcatgc caccgttgtg gcctctgtac atggtgccct tgtctgtacc gccattctcg
    41461 ttaagcactt cggtacggcc gtttctggtg agctgttcag agaagaacat ggcggtataa
    41521 agccagcgcc agtacatgat tgtcttttcc ggcgcgagct cacctgtcat gcctttttct
    41581 tctgcttcac tgatgcaaca cactatttcc agaccatgcc agatcggttc gtcaaaacag
    41641 ccttcatcga gcatttccag tgctttgttg atatcaagca gtccgtaagg tgtgtggata
    41701 ccttcagcag taagaaaaaa taactgttca gttgctgtca tggtttgctc tcttcttaaa
    41761 tggtctttgc gcgacaagcc ggcaatactg atcaatccag gcgttgtatt tttccatcca
    41821 ggcatcaaac tgttttctgc gtgcaaggat gcgcaacagg cgatgaaaac aacgcccgtg
    41881 agctctgtag tattcgggcg taatttctcc cggatgaaag acttctccat attccggatg
    41941 catcaggcgc tggtcaggca gacgtttttc aaaacctgaa ctttcgaata ccttcgttgt
    42001 cataaaatta gcgagatgta tgatggccgt ttttcgcgtg aagcatttct taacttgtcc
    42061 gtggcggcag actctgtaaa cagggccgca atcgagttcg tatttatgaa aagcacggtc
    42121 gattccggca ttatttattg cactgtattc cgtatcactg aagaaaggtg gaaatacgat
    42181 gtatccttcc attgtcagcg ccagaacgcg cggatcggtt tctgtcagcc attcgttttc
    42241 tgtgaattct tcagaattaa tttcaatgat ttcaggttct tctttcatgg ttactccgtg
    42301 ttcatccggt gttaattact tttattcact ctgagcgcag ccagaagatg tggctcagca
    42361 attcgtttgt tgcgatatat ttgtgataca gcctggcctt ttcaggttgt aaaaatcccc
    42421 gacaataaag ctgtaaatta attactgttc ttaaataatg ttattcgttt tcagtccggc
    42481 acagatgctt ttctgctttg tctgctgtac aggataatga gcggaaaaga caattcatca
    42541 tgtttagcgc atctggtgaa ccaatatcac cgctgtcaat aatcacctgt aacaaagaca
    42601 ggctttctgt tatctcgcag tggacattat cgagtacttc aacgcaatta ttcatttttc
    42661 attatctccg ttattccggg ggaggagcta tgctgttaat gattaacgca tccggtaata
    42721 tgcccctctt taatgtaatc gatgcattca ttcaaaagtt catcaatgat taactttcct
    42781 gattcagtca gatactcacc atgttggttg ataccgacag catcctggta tgcagtgcgt
    42841 attgctgttt caccatcttt ccgcccaaac tcaccacggg taacgccttc aaatcgtaat
    42901 agcaactggc ttatgaactg ttctgttatt tcaatagttg ttaactctcc atcatgacgc
    42961 tttacagtga ggagattact tcctgttttt cgtttcatcc gccgcagtgc tgcaaccgtt
    43021 atgcggcgac gatatgcaga aatgtattca tctttcattt gttatttccc gtatgccttt
    43081 ttgagaaaca atactgcaac tgaccagtat ccggcatcag ccattaataa agcggtttta
    43141 taggcatgtt tgtctttcat gtgtcatccc cattttaaaa tagtgagaac tcccgaccgt
    43201 agaccgtcat gtttttatgt atgattgtct gtttaatttc ctttattcgt tctcagagca
    43261 tcaacatact cgtaagcctt ttcacaggtt ttattcatgg aacgaatcag acaaagtaag
    43321 aaatcatctg tctctccgtt atcaccagag tttttaaata taaactcaag cattgacgtg
    43381 ttttctttta tttctgctgc cacttcctca agcataatca ggggagtttt catgttcttt
    43441 gttccttaaa tgcatcgcat gcgcttctgg cgtattgttg tgccagtaaa aagatgtcat
    43501 ccgaaagttc atcacattct tcatcaccgg aagccgaaat gattaacccc gcttcaagca
    43561 gtactgcaat gtgatgaaaa gctgttttcg gttcgttggt gaggcctttg aacattttca
    43621 tcttacgatt cctttgtcgt gtcagaatgg aaacttgtaa gtaaccacat cggatcgcag
    43681 tcaagtatat ttgccagagg aatcacctgg cttgccagag gttcagcctt gccgttctcc
    43741 cactggatga tggtttcttc atcaactcca agcagcgtgg cgagttcagc ggtggataga
    43801 ccgcagcttt cgcgttgggt gcggatgcca tcacggcaat cttttttgtt tctcggaggt
    43861 aacagaatat gttttagttg ttggattttt tcgattccaa cctcaaggag tacgtgacgg
    43921 tttctttccg atacagactc gagactttca tgaagcagcg attctacttg ttcgataagc
    43981 atcaattgtg aagtagacat aaaaaaacct cgtaaccaat atttcgaaat ctaggttacg
    44041 agtgagtaat gtaggtgtca agagcaatta atgttttttg ttgaattaaa tttgcatacg
    44101 catctcaacg acaacaccaa taatttcgca atccccattg atggggatca gtggatagcg
    44161 tgggtttagt ggctttagga aacattgccc agcgtcttct atgtactgtt taagggttgc
    44221 ctctccggag tttttgagcc tggcgacgac atattttcct gaaaaaactt ctttgtcagg
    44281 gttaacaaga atgttcatgc cttcaaaaat agagaactct ccattctgtg atgtcattga
    44341 gtcccctttc acttccagcc agaagccgtg atcgcccgca tatatatcac taccaatcca
    44401 ttcttctgga taagctatgt aatcttcgca gttccagggg ccagcactca cccatgttag
    44461 tttcggatat ctataacggt ttggagaaac tggcttagga tttcggacgt tttgaatgct
    44521 atctgtaggt gatggggcgc tgccgtcaag aagccattta ggagagcatt tcaatgcatc
    44581 cgcgattcta aataggtttg aagatcttgt gtcttgtgag tctcctaact ctattttgct
    44641 gattgttaca cgcgagacac cagtcaattt tgccaggtca tcttgtgaca ttcctagctg
    44701 tactctgcgc attaacagtc tgccaccaaa gctatcgtga tccatcttct cccccttctt
    44761 gtcgaaataa tcgtttcgaa acaaatatag caaaaaatct tgaaatctag gtttcggaaa
    44821 attaagatta atcgaaacaa acgtgtcgag gtggatatgg atttgtatga aattctaaaa
    44881 aacatgtttg gatcgaatgt tgaaataggt cgttattttc cccgaagggg aagggctaga
    44941 accggacagg ctgtaggtaa gtggaaaacc cgaggtgtgc cggaagatgt ggcgatcctt
    45001 tgtcatctgg atcccaaaat tccttatcag catccgtcgc tcatgaatgc cagtcatgaa
    45061 tcgtgatgtt ctgccggagg ctgtatgtcc cctgattacg ttcagatgga gatgcctgcc
    45121 aggtacagcc aggcagattc agagtggatt aagcggcagt tacagagcct gccttcctca
    45181 ctgagacaga aggtcgccct gaaatacgcg gaggtttacg aagttacttt tgattccgag
    45241 cctgtttcat tccgtaagga gaacagagcg aggcatgaag ccaatgtacg gctgcgcagg
    45301 ttcgttgaaa cacacgggcg tgcattacag gggtatacga ctcagccgcc cctggcagga
    45361 acgcaacagc gtacctgaat ggtgttggtc ttaaaggtga ccgacgggct gagaccccac
    45421 ttctcaggat gcacctgtgt actaccaaac ta
//
LOCUS       DAZGSP010000033        45283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZGSP010000033 DAZGSP010000000
VERSION     DAZGSP010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.033"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            60..995
                     /locus_tag="ACHK74_001968"
     CDS             60..995
                     /locus_tag="ACHK74_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP0220794.1"
                     /translation="MLTKRILFSLMLIVSPGVVASEKAHELYDSIYGGKPAPNVINTL
                     HKMAESGDADAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKHGDREAPLALGSIYYD
                     GEQVRVDYAKAYALFNQAAQYGVNLAWSRLGMMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDEGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(1051..1593)
                     /locus_tag="ACHK74_001969"
     CDS             complement(1051..1593)
                     /locus_tag="ACHK74_001969"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0220795.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1577..1888)
                     /locus_tag="ACHK74_001970"
     CDS             complement(1577..1888)
                     /locus_tag="ACHK74_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFP0220796.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(2073..2963)
                     /gene="cyoE"
                     /locus_tag="ACHK74_001971"
     CDS             complement(2073..2963)
                     /gene="cyoE"
                     /locus_tag="ACHK74_001971"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFP0220797.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRVWARKLFGFSIVAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(2975..3304)
                     /gene="cyoD"
                     /locus_tag="ACHK74_001972"
     CDS             complement(2975..3304)
                     /gene="cyoD"
                     /locus_tag="ACHK74_001972"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFP0220798.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(3304..3918)
                     /gene="cyoC"
                     /locus_tag="ACHK74_001973"
     CDS             complement(3304..3918)
                     /gene="cyoC"
                     /locus_tag="ACHK74_001973"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFP0220799.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(3908..5899)
                     /gene="cyoB"
                     /locus_tag="ACHK74_001974"
     CDS             complement(3908..5899)
                     /gene="cyoB"
                     /locus_tag="ACHK74_001974"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFP0220800.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(5921..6868)
                     /gene="cyoA"
                     /locus_tag="ACHK74_001975"
     CDS             complement(5921..6868)
                     /gene="cyoA"
                     /locus_tag="ACHK74_001975"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFP0220801.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(7328..8803)
                     /gene="ampG"
                     /locus_tag="ACHK74_001976"
     CDS             complement(7328..8803)
                     /gene="ampG"
                     /locus_tag="ACHK74_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFP0220802.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(8847..9425)
                     /gene="yajG"
                     /locus_tag="ACHK74_001977"
     CDS             complement(8847..9425)
                     /gene="yajG"
                     /locus_tag="ACHK74_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0220803.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            9730..10047
                     /gene="bolA"
                     /locus_tag="ACHK74_001978"
     CDS             9730..10047
                     /gene="bolA"
                     /locus_tag="ACHK74_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFP0220804.1"
                     /translation="MMIRERIEEKLRVAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            10391..11689
                     /gene="tig"
                     /locus_tag="ACHK74_001979"
     CDS             10391..11689
                     /gene="tig"
                     /locus_tag="ACHK74_001979"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP0220805.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            11935..12558
                     /gene="clpP"
                     /locus_tag="ACHK74_001980"
     CDS             11935..12558
                     /gene="clpP"
                     /locus_tag="ACHK74_001980"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFP0220806.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            12684..13958
                     /gene="clpX"
                     /locus_tag="ACHK74_001981"
     CDS             12684..13958
                     /gene="clpX"
                     /locus_tag="ACHK74_001981"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFP0220807.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            14146..16500
                     /gene="lon"
                     /locus_tag="ACHK74_001982"
     CDS             14146..16500
                     /gene="lon"
                     /locus_tag="ACHK74_001982"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFP0220808.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            16691..16981
                     /gene="hupB"
                     /locus_tag="ACHK74_001983"
     CDS             16691..16981
                     /gene="hupB"
                     /locus_tag="ACHK74_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFP0220809.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            17173..19044
                     /gene="ppiD"
                     /locus_tag="ACHK74_001984"
     CDS             17173..19044
                     /gene="ppiD"
                     /locus_tag="ACHK74_001984"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP0220810.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            19195..19566
                     /gene="ybaV"
                     /locus_tag="ACHK74_001985"
     CDS             19195..19566
                     /gene="ybaV"
                     /locus_tag="ACHK74_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFP0220811.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            19660..20058
                     /gene="fadM"
                     /locus_tag="ACHK74_001986"
     CDS             19660..20058
                     /gene="fadM"
                     /locus_tag="ACHK74_001986"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFP0220812.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(20110..20805)
                     /gene="queC"
                     /locus_tag="ACHK74_001987"
     CDS             complement(20110..20805)
                     /gene="queC"
                     /locus_tag="ACHK74_001987"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFP0220813.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(20870..22570)
                     /gene="ybaE"
                     /locus_tag="ACHK74_001988"
     CDS             complement(20870..22570)
                     /gene="ybaE"
                     /locus_tag="ACHK74_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFP0220814.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            22670..23488
                     /gene="cof"
                     /locus_tag="ACHK74_001989"
     CDS             22670..23488
                     /gene="cof"
                     /locus_tag="ACHK74_001989"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFP0220815.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            23641..24099
                     /gene="decR"
                     /locus_tag="ACHK74_001990"
     CDS             23641..24099
                     /gene="decR"
                     /locus_tag="ACHK74_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFP0220816.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            24129..25901
                     /gene="mdlA"
                     /locus_tag="ACHK74_001991"
     CDS             24129..25901
                     /gene="mdlA"
                     /locus_tag="ACHK74_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP0220817.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVAQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            25894..27675
                     /gene="mdlB"
                     /locus_tag="ACHK74_001992"
     CDS             25894..27675
                     /gene="mdlB"
                     /locus_tag="ACHK74_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP0220818.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            27856..28194
                     /gene="glnK"
                     /locus_tag="ACHK74_001993"
     CDS             27856..28194
                     /gene="glnK"
                     /locus_tag="ACHK74_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFP0220819.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            28224..29510
                     /gene="amtB"
                     /locus_tag="ACHK74_001994"
     CDS             28224..29510
                     /gene="amtB"
                     /locus_tag="ACHK74_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFP0220820.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(29559..30419)
                     /gene="tesB"
                     /locus_tag="ACHK74_001995"
     CDS             complement(29559..30419)
                     /gene="tesB"
                     /locus_tag="ACHK74_001995"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFP0220821.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            30637..31209
                     /gene="ybaY"
                     /locus_tag="ACHK74_001996"
     CDS             30637..31209
                     /gene="ybaY"
                     /locus_tag="ACHK74_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFP0220822.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(31240..31551)
                     /locus_tag="ACHK74_001997"
     CDS             complement(31240..31551)
                     /locus_tag="ACHK74_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFP0220823.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            31714..31810
                     /gene="ffs"
                     /locus_tag="ACHK74_001998"
     ncRNA           31714..31810
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHK74_001998"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            31930..32283
                     /gene="ybaA"
                     /locus_tag="ACHK74_001999"
     CDS             31930..32283
                     /gene="ybaA"
                     /locus_tag="ACHK74_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFP0220824.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(32325..33875)
                     /gene="pdeB"
                     /locus_tag="ACHK74_002000"
     CDS             complement(32325..33875)
                     /gene="pdeB"
                     /locus_tag="ACHK74_002000"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFP0220825.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(34039..34509)
                     /gene="ylaC"
                     /locus_tag="ACHK74_002001"
     CDS             complement(34039..34509)
                     /gene="ylaC"
                     /locus_tag="ACHK74_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFP0220826.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(34629..35177)
                     /gene="maa"
                     /locus_tag="ACHK74_002002"
                     /pseudo
     CDS             complement(34629..35177)
                     /gene="maa"
                     /locus_tag="ACHK74_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(35348..35566)
                     /gene="hha"
                     /locus_tag="ACHK74_002003"
     CDS             complement(35348..35566)
                     /gene="hha"
                     /locus_tag="ACHK74_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFP0220827.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(35592..35966)
                     /gene="tomB"
                     /locus_tag="ACHK74_002004"
     CDS             complement(35592..35966)
                     /gene="tomB"
                     /locus_tag="ACHK74_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFP0220828.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(36512..39661)
                     /gene="acrB"
                     /locus_tag="ACHK74_002005"
     CDS             complement(36512..39661)
                     /gene="acrB"
                     /locus_tag="ACHK74_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFP0220829.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(39684..40877)
                     /gene="acrA"
                     /locus_tag="ACHK74_002006"
     CDS             complement(39684..40877)
                     /gene="acrA"
                     /locus_tag="ACHK74_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFP0220830.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            41019..41666
                     /gene="acrR"
                     /locus_tag="ACHK74_002007"
     CDS             41019..41666
                     /gene="acrR"
                     /locus_tag="ACHK74_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFP0220831.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            41794..45156
                     /gene="mscK"
                     /locus_tag="ACHK74_002008"
     CDS             41794..45156
                     /gene="mscK"
                     /locus_tag="ACHK74_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFP0220832.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKQASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
BASE COUNT        11481 a        11119 c        11922 g        10761 t
ORIGIN      
        1 tggatttttg gtggttaatt attatggaaa tatatgacag agagaacata aaggtataca
       61 tgttaactaa aagaatacta ttttctttaa tgctaatagt ttcaccaggc gtggtggcaa
      121 gcgagaaagc acatgaattg tatgacagca tatacggtgg taaacctgcg cccaatgtta
      181 taaatacact gcataaaatg gctgaatcgg gggatgccga cgcgcagagc ctgttaggct
      241 gggaatatta tcagcctcgc tatgatacca aacctgatgt tcaggaagcg attaaatggt
      301 tcgagttagc cgctaaacac ggtgacagag aagctccgtt agcgctgggg agtatctact
      361 acgacggcga gcaggtgcgg gtggattatg ccaaagcgta tgcgctattt aatcaggccg
      421 cgcaatacgg tgtgaattta gcatggtcca ggttgggcat gatgtacgcc aatggtcagt
      481 atgttgaggt agattgtaag aaagcgaagg aatatcttga taaaggtgtc cacatttatg
      541 gtggcccgga agactttctg gctacttgtc gaaaagacat gattgacaga aaaacggttg
      601 acgatacgtt acccgtgatc acggttaccc gttctgggat gcgggataat tttttagatg
      661 aaggtttttc ctgcatggat agcctcttcg ccaccaccaa taaattaggc gaagtggcca
      721 accttcgcgt gacattcagc attcgtcgcc catccggtaa ggagattaac caaacggttg
      781 gcttcgcgcc tttcggttta aaccggctga atattagctt tactgattac ctatttggtt
      841 catttaccag taattcgtct cttattcttt ataaaccgga atttgagcga aaatcttgtg
      901 caactgtaag gaccacaatc gttgccgcaa cggcaaccat taacggcaag gatgtggagt
      961 tgctgaaagc gggggcaatt gaacaaaagt ggtaacgctg ctcagccccc ggctgaggca
     1021 aaacttgccc tggcgggtgg cgataataat ctaagccgcg agaatatgct gaatcgccaa
     1081 aaacataggt acagcaccaa aagcatttgg tgtcgctgaa agctgaacaa agccgagacg
     1141 agcataaaaa ttaatggcgg cagggtcagc atcaagataa acccctttaa ttggtaatgc
     1201 ctggtgaatt atttttacat gttcaaaaaa atcacatagt aggtcctgac caaagccccg
     1261 cttttgatac ttccgtgcta cccccaacat gactaatctg acaacaccaa tttctgaagg
     1321 ttgtgaaccc tgaaggacgc cagaaacgcg ttgtttttcc agcgaatatg ccgtaaaagt
     1381 acagatgccg atcagttcac cactttgtct gtcaataagt gctttagccg cacagtcgct
     1441 attacgcaca cttttcttta gcgatgcgcg tacaaattta tcgataacag ggttaccgca
     1501 atcaaactgt ttttgcccag gataagtaat atcagactga taattacagg agaggactat
     1561 gggcagagta atctcttcat gcttgtcgac catcgctgtt tttccttttc attaacgcct
     1621 tgagggcgag cgtcggttga gcaggttcag caatgagttg gttcatcagt tcccagcttt
     1681 gattcgaaag ctcacggcga cgttggttat caagcacact ttcggcgcgt tccatagcag
     1741 cgctcaaaat aaaagcgctt aaatctaccc caacggcttg cgcagcttca cgaagtttgt
     1801 ttttggcgtc gggactagtt ttgagttcga cccgcgcatt tttcagcgtt ttatcgacat
     1861 cgggtagagt ggctgttttt ccaggcatta tgacctcttt gattaactga atgaaatcgt
     1921 agggagtcga tctcatacat aatacggcta tcaaccgtat gattaattta tgttcatctg
     1981 ctcactctgt caagacatac ggttattagc cgtatctctg atctcagaca ataaaaaagg
     2041 tgccgtagca cctttttaat agagaggttg tgttaccaaa cagcagccag cagcgtatgc
     2101 gagtccggca ccataaaatc aacggacatc atcaccgaga gggcagtgat ggcgactatc
     2161 gagaagccga acagcttgcg cgcccaaact ctgtcatcag caactttata accgcgcagg
     2221 gccataccta accaccagac gctaaccgcc gcggcgacca ccagatattt atacccagcg
     2281 taaccgccaa gagagagcat cagcgtggca acggcaaagg cgatgatata cagcgtgatg
     2341 tgattcttcg ccaccgaaat gccttttacc actggcaata ccggaatgtt tgccgcctgg
     2401 taatccttaa aacggaaaat ggcgatggca taggagtgag gcatctgcca caggctgaag
     2461 atagccagca ggatcgctgc gccgctatcg aactcaccgg ttaccgcaca gtagccgatc
     2521 accggcggcg cagcgccgga gagcgaacca atcaacgtgc cgtagaccga gtggcgtttc
     2581 atgtacaggc tataaacgcc gacataaacc acaaagccca tcacccccag ccagcaggcc
     2641 agcggattcg cgccaaacca cagcagcata aagccagcaa tacccagcaa cgtggcgtac
     2701 accagcgaga cagcaggaga gatcaggcct ttcaccagca cccgattctt cgtcctttcc
     2761 atctttctgt cgatatccct gtcgatgtag ttgttaaaca cacaacccga cgccacaacc
     2821 agtgacaccc caaccagcgt gtagataaac aggggataat caatgctgcc ctttgaggcc
     2881 agcaggaatc ccccaatcac cgagatcagg ttgccaaaga tgatgcctgg tttcgttact
     2941 tgcaggtatt gcttaaacat cataaccgcc gctcttagtg catcatcatg ttgtagttga
     3001 ggttccacat aatccagatg gagcctacaa ccaggatagc gatgattagc acggtgaaga
     3061 caaacgccgt catgttccag ccttcatctg atttggtatt catgtgcagg aagcacacca
     3121 gatgcaccag aacctgtacc actgccattg ccaggattgt tcccagaatt acggccggag
     3181 aggcagctcc tgtcatcacc atccagaacg gaatcaccgt cagaatgatc gacaggataa
     3241 agcctgtcat gtaggttttt acgctgccat gggacgcgcc gctgtgatcg gtagaatgac
     3301 tcattacatc gcccccatca gataaacaac agtgaacaca cagatccaaa ccacatccag
     3361 gaagtgccag aacaggctca ggcacatgat gcgggtacgg ttagtgctgg tcaggccgcg
     3421 acgggcgatt tgcaccatca gcaccgccat ccagataaga ccagaagtga cgtgcagacc
     3481 gtgcgtgccg accagcgcaa agaacgctga caggaagccg ctgcgatccg gacccatgcc
     3541 gttaacaatc aggtgatgga attcatagat ttccatcccg ataaatccgg caccaaacaa
     3601 ccaggtcaac gccagccagg agataacctg gcttttgttg tttttgtaca tggcgatagc
     3661 cgccatgccg taggtgatgg agctgaacaa cagcaagaaa gtttcaacca gaacgaacgg
     3721 cagttcgaaa atgtccttac ctgtcgggcc gcctgcggtg ccgttcacca gaacggcata
     3781 ggtagcaaac aagatagaga acagaatgca gtcgctcatc aggtagatcc aaaatccgaa
     3841 gattttggtt ccgcctgcat cgtggtgccc gtgttcgtgc gcgtgggcag tcgcgtgcgt
     3901 caaagtatca gttgccattt ttcagccctg ccttagtaat ctcatcgaaa tgctggtttt
     3961 ccagtttttc gatttctgcc accggcacgt agtaatccac gtcctcgtcg aagcttttca
     4021 cgatccaggt gatgatcatg cctgcgaagc caacaatcgc cagccaccag atatgccaga
     4081 tcatggcgaa accgaagatg gtggagaaag ctgcaatgac gatacctgca ccgctgtttt
     4141 tcggcatatg aatttcttca tagtggtcag gctttttata cgcttcgcct ttctctttca
     4201 tttcccagaa tgcatcacgc tcgtgaacgt gcggcacaac ggcaaagtta tagaacggag
     4261 gcggggaaga ggttgcccac tccagcgtac ggccacccca cgggtcgcca gtcaggtcac
     4321 ggttctggtc gcggtcacga atagaaacgt acatctgaat aacgaggcag agaataccta
     4381 gcgcaatcag tactgcaccg ctggctgcaa tcatcagcat ggtgtggaac tgcgggtcaa
     4441 tctgctggct caaacgacgg gtcatgccca tgaagcccag cgcgtacagt ggcataaagg
     4501 caacgaagaa gccgatgatc cagaaccaga acgcgcgttt accccaggtt tcgttcagtt
     4561 tgaaaccgaa cgctttaggc caccagtagg tcatccctgc gaagcagccg aagaccacgc
     4621 cgccgatgat cacgttatgg aagtgcgcaa tcaggaacag gctgttatgt agaacgaagt
     4681 ccgcgcccgg tactgccagc agcacgccag tcataccgcc caccgagaag gtgacgataa
     4741 aaccgatagt ccacagcatc gcagaatgga acacgatgcg gccctgatac atggtgaaca
     4801 gccagttgaa gatcttcacc ccggtcggga tggcgataat cattgtggtg ataccaaaga
     4861 aggcgtttac gttcgcgccc gcacccatcg taaagaagtg gtgcagccaa acgatgaacg
     4921 acagcacggt gatacagacg gttgcccata ccagcgaggt ataaccaaac agacgtttac
     4981 gcgagaaggt tgccgcaatt tcggagaaca caccgaaaac aggcaggatc aggatgtaaa
     5041 cttctgggtg gccccaggcc caaatcaggt tgatgtacat catcatgttg ccacccatat
     5101 cgttggtaaa gaaatgggtg cccagatagc gatccagggt caacaacgcg acggtaaccg
     5161 tcagaattgg gaaggaagca ataatcagta cgttcgcgca cagtgatgcc caggtaaata
     5221 ctggcatctt gaacatggtc atgcccggtg cgcgcatctt cagaatggta acgaagaagt
     5281 tgataccggt aagcgtcgta cctataccgg atagctggag actccatatc cagtaatcga
     5341 caccgactcc cggactgtac tctattcccg atagcggtgg ataggccagc cagccggtct
     5401 gcgcaaattc gcccacgccg agagaaacgt taaccagaat cacaccaaca acagtaaacc
     5461 agaagcttaa gttgttgagg aacgggaacg caacgtcacg cgcgccgatc tgcagcggaa
     5521 ccaccaggtt catcagaccg ataacgaaag gcatcgccac gaagaagatc ataatcacgc
     5581 cgtgcgcggt gaagatctga tcgtagtggt gaggtggcag gaagcccgct tcgcccgccg
     5641 aggcaagagc ctgctggcta cgcatcataa tggcgtcagc aaaaccacgc agcaacatca
     5701 caatcgccac gatgatatac atgataccga ggcgtttatg gtcgacggag gtcagccact
     5761 ctttccacag gtaggtccac ttaccgaagt aagtgatcag gccaacgagc gccagacctc
     5821 ccaaaataat gccagcgatc gtaaccatga cgataggttc atggaacggg actgcatcaa
     5881 gtgataattt tccgaacatc tttattcttc ctcaacccct ttaatgggcg gattccgcgt
     5941 ggctcatgtc catgccttcc ataccttcgt gtgcgctgtg ctcaccttct ggctgggtca
     6001 tgtccatgct cttaccgtga gccataaact tgttaatcac atcggcaaac aagtctggtt
     6061 tcacgttgga gaaatattcc acctggttgt attcgctagg cgcggccagt ttttcgaacg
     6121 cagccatgtc agacatggtg ttcggcgact gtttcgcttt tgcgacccat tggtcgaatt
     6181 cggcgcgatc cggtgttgca atagctttga acttcatgcc tgagaagccc gggccgctgt
     6241 agctggcgga gataccgtca taagtaccgg gttcgttggc gatcagatgc aggcgagtct
     6301 gcataccagc catggcataa atctggctac ccagacgcgg aatgaagaag gagttcatca
     6361 cggagttgga ggtcactttg aagtacaccg gagtgttcgc cgggaaagcg atttcattca
     6421 cggttgcaat gccctgttcc gggtagatga agaaccattt ccagtccatg gaaaccactt
     6481 cgatggtaat gggcttctcg tcgtgtgcca gcggcttgct aggctcaaga gcgtgagtgg
     6541 ttttccaggt cagtaccgca aggaagatga tgattaagat aggtaccgtc cagaccacag
     6601 cttccacttt attggagtgt gaccagttcg ggctgtactt agcatcttta ttgctcgcac
     6661 gatacttcca ggcgaaacca acagccatca agattgcggg aataacgaca atcaacatca
     6721 ggccaaatgc cgtcagtatc agtgaacgtt gctccagacc aatctgtcct ttgggatcta
     6781 acagcgcaga attacagcca ctgagcaata cagtgcctgc aaataatgac aaccatccca
     6841 aacttttatt gtatttcctg agtctcattt aacgacctca attccacggg atctggtggc
     6901 gtttaaagtg tgtgggcatt ttacgggaag gttacattac tgtaaacatg attaaattcc
     6961 tgttacttgg atttggctgt caggtcacat atgttgcaaa acatatcaag ttgattttaa
     7021 gaagctgttg caaaagggcg ttataacgaa aggggagtgc agaaggagtt tggacgggca
     7081 attggtataa ccaatacaaa ataaaacaat tatttaacaa ataattatca ttaaggtgac
     7141 aactgggtag tgagaaaata aaaactaatt agctgaatcg tgtaagaaaa atagaattca
     7201 taaatagcgt caataatttc caaacgctaa gccgcacaaa agaacaaata ttaataagcg
     7261 gtgctgatgt ttatttagcc gtaataatta cggcgggtga ttactacagc taaataatat
     7321 ttacagatta cgtcagatgc gtttttcgta gcgccagata atccagcaaa ccaccaagca
     7381 cgacgccaga aagggcgact aaaaccccga cttccagcag agcaggcagg aaagagaagt
     7441 gcgtcaaatc cagcgcgtcc atcgtcaaca gcagtaacca cacggccaac aggctgacgc
     7501 ccgccgccag tgtccacatg gcaaaggcat aacctgccgg atattcggta cgggagataa
     7561 agttgtcatt tactcgtgta tattcaagcg tctggcggca aaccagcagc aaaataagcc
     7621 ccggtacggc agcggcgacg gagaatagat agaatgtcga ccagccgtgt gcttcaacaa
     7681 accaacccgc cacggggccg acataaactc gccctacagc ggaaagcgct gagagcaggg
     7741 caaattgagt tgcggaaaat gacttattac acagcgtcat taacagcgcg acaaaggctg
     7801 atgtgcccat cccgccacag aggttttcga aaaagacggc tgcgcccatg ctgtagagat
     7861 gcttatcagt aatcgacagc agccagtaac cagcgttaga cgcaccttgt aaaatgccga
     7921 aaatcagcag cgcccggaac agcgacaggc gctgcatcaa aatcccaccg tacaatgcgc
     7981 ccacaatggt cgccagtaag ccaagcgttt tgttaaccac gcctacttca cccgcatcaa
     8041 acccgacgcc gcgaatcaaa aacgtggttg tcaggctcat ggcgaatgcg tcgcccagct
     8101 tatacagcac gataagaagc aaaataagcc aggcattatt gcgaccaaag aaatctcgca
     8161 gaggtgcaac aaccgcttgt tccagcgttt ttggcacagg aatggtgtcg gtcggttctg
     8221 gtgcaagcaa cgtcgcgata atacaaggga tcaacagcgc cgccatcaac cagtacatgc
     8281 cctgccagcc cagccattta tctgccagcc acagggctaa gccgccggaa accagcatcc
     8341 ctaaacggta acccagcacg ctgattgccg cgcccgcacc acgttcttct gccgggagca
     8401 catcggtttt ccacgcatcg aagacaatat cctgggaggc cgagcaaaaa gcgatcacca
     8461 ccgccagtgc cgccatccag cggagttggg tgccaggttc gagaaaaccc atcgccgcaa
     8521 tggcgactaa taacaggatt tgcgtggcga gcagccaacc gcgccgccgc ccaaaaaatg
     8581 ggggcgtgta gcggtccatc agcggtgacc agaggaattt aaaaacgtaa gcctggccta
     8641 ccagagagaa gaaaccaatg gttttgagat caatattctc gaccgtcatc caggcctgta
     8701 aggtgccgga tgtcagggcg agcggtagcc cggaagcaaa acccaggatc agcaatatgg
     8761 ctgaacgcgg ctgttgaaaa atacgtaaat attgactgga catgggcgtc tacaggcccg
     8821 gcttgcaccg ggccagaggg cagaaattaa cgcgcgttct gcttgatgaa ttcgtgaatg
     8881 ctggtgtcct gagacatatc agcgatggta tcggtcagca cactgttaac cgcatcggcg
     8941 atatttttgt tggaggcctg gaacgcacct tcaacgttgt agctggcacg atagtttttg
     9001 gtcattttgt tgccattctg cgcggtagcg atgatggcga tatccgcttt ggtagcgatg
     9061 ttgtagcgca cgttgccctg ggacacgtca gcatacagtt ggctaacgat gatttgcaga
     9121 ttaaccgggc cattcggacc aaccatgtaa ccacgcgcgg tcatctgttt ttccagcact
     9181 tcttgcagca ggaaacgcag atcgcgggag gcggtcaggg taacgatttg attatcgcgg
     9241 gtgacttttg ccagcgcctg atcggtacgc tgatcggcac cattaatgct tacggtgacg
     9301 cccatcaggc ttggatcctg ctgtggcagt gtaatcgtcg gggaaacttc aatagttgtt
     9361 ggcggttttg cgcatcctgc aagcataaac agagcaacta acgggaagag gatttttttg
     9421 aacatgttcg ggctctcaga gactcttaag cgtgtttggt aaaaattccc gccatcataa
     9481 cattgccaac ggcgagggga agtgggtaag gcatgtaaat tcatcatgtt gacgaaataa
     9541 tcgcccctgg caaaagaaac actgatgcga ggcctgtgtt tcaatcttta aatcagtaaa
     9601 cttcatacgc ttgacggaaa aaccaggacg aaacctaaat atttgttgtt aagctgcaat
     9661 ggaaacggta aaagcggcta gtatttaaag ggatggatga catctcagcg ttgtcggagg
     9721 agatatttca tgatgatacg tgagcggata gaagaaaaat taagggtggc gttccaaccc
     9781 gtattcctcg aagtagtgga tgaaagctat cgtcacaatg tcccagccgg ctctgaaagc
     9841 cattttaaag ttgtgctggt cagcgatcgt tttacgggtg aacgttttct gaatcgtcat
     9901 cgaatgattt acagtacttt agcggaggaa ctctctacta ccgttcatgc gctggctctg
     9961 catacttaca ctattaagga gtgggaaggg ttgcaggaca ccgtctttgc ctctcctccc
    10021 tgtcgtggag caggaagcat cgcgtaaaaa cgcatttgca actgtcggcg cttttccagt
    10081 atgttgctaa agattttatg aaaaacggcc tgcgggccgt tttgttttgt ctggattttg
    10141 cgctttttgc ccagcattca gacgaaaatt gcccgggaat tgtgaaaaaa tacgcgacag
    10201 cgcgcaataa ccgttctcga ctcataaaag tgatgccgct ataatgtcgc gtcctatttg
    10261 aatgctttcg ggatgattct ggtaacaggg aatgtgattg attataagaa catcccggtt
    10321 ccgcgaagcc aacaacctgt gcttgcgggg taagagttga ccgagcactg tgattttttg
    10381 aggtaacaag atgcaagttt cagttgaaac cactcaaggc cttggccgcc gtgtaacgat
    10441 tactatcgct gctgacagca tcgagaccgc tgttaaaagc gagctggtca acgttgcgaa
    10501 aaaagtacgt attgacggct tccgcaaagg caaagtgcca atgaatatcg ttgctcagcg
    10561 ttatggcgcg tctgtacgcc aggacgttct gggtgacctg atgagccgta acttcattga
    10621 cgccatcatt aaagaaaaaa tcaatccggc tggcgcaccg acttatgttc cgggcgaata
    10681 caagctgggt gaagacttca cttactctgt agagtttgaa gtttatccgg aagttgaact
    10741 gcagggtctg gaagcgatcg aagttgaaaa accgatcgtt gaagtgaccg acgctgacgt
    10801 tgacggcatg ctggatactc tgcgtaaaca gcaggcgacc tggaaagaaa aagacggcgc
    10861 tgttgaagca gaagaccgcg taaccatcga cttcaccggt tctgtagacg gcgaagagtt
    10921 cgaaggcggt aaagcgtctg atttcgtact ggcgatgggc cagggtcgta tgatcccggg
    10981 ctttgaagac ggtatcaaag gtcacaaagc tggcgaagag ttcaccatcg acgtgacctt
    11041 cccggaagaa taccacgcag aaaacctgaa aggtaaagca gcgaaattcg ctatcaacct
    11101 gaagaaagtt gaagagcgtg aactgccgga actgactgca gaattcatca aacgtttcgg
    11161 cgttgaagat ggttccgtag aaggtctgcg cgctgaagtg cgtaaaaaca tggagcgcga
    11221 gctgaagagc gccatccgta accgcgttaa gtctcaggcg atcgaaggtc tggtaaaagc
    11281 taacgacatc gacgtaccgg ctgcgctgat tgacagcgaa atcgacgttc tgcgtcgtca
    11341 ggctgcacag cgtttcggtg gcaacgaaaa acaagctctg gaactgccgc gcgaactgtt
    11401 cgaagaacag gctaaacgcc gcgtagttgt tggcctgctg ctgggcgaag ttatccgcac
    11461 caacgagctg aaagctgacg aagagcgcgt gaaaggcctg atcgaagaga tggcttctgc
    11521 gtacgaagat ccgaaagaag ttatcgagtt ctacagcaaa aacaaagaac tgatggacaa
    11581 catgcgcaat gttgctctgg aagaacaggc tgttgaagct gtactggcga aagcgaaagt
    11641 gactgaaaaa gaaaccactt tcaacgagct gatgaaccag caggcgtaat ttacgcagca
    11701 taacgcgcta aattcgcaca aaggcccgtc accgccaggt ggtgggcttt tttttgtcat
    11761 gaattttgca tggaaccgtg cgaaaagcct ctttcggtgt tagcgtaaca acaaaagatt
    11821 gttatgcttg aaatatggtg atgccgtacc cataacacag ggactagctg ataatccgtc
    11881 cataaggtta caatcggtac agcaggtttt ttcaatttta tccaggagac ggaaatgtca
    11941 tacagcggcg aacgagataa ctttgcaccc catatggcgc tggtgccgat ggtcattgaa
    12001 cagacctcac gcggtgagcg ctcttttgat atctattctc gtctacttaa ggaacgcgtc
    12061 atttttctga ctggccaggt tgaagaccac atggctaacc tgattgtggc gcagatgctg
    12121 ttcctggaag cggaaaaccc agaaaaagat atctatctgt acattaactc cccaggcggg
    12181 gtgatcactg ccgggatgtc tatctatgac accatgcagt ttatcaagcc tgatgtcagc
    12241 accatctgta tgggccaggc ggcctcgatg ggcgctttct tgttgaccgc aggggcaaaa
    12301 ggtaaacgtt tttgcctgcc gaattcgcgc gtgatgattc accaaccgtt gggcggctac
    12361 cagggccagg cgaccgatat cgaaattcat gcccgtgaaa ttctgaaagt taaagggcgc
    12421 atgaatgaac ttatggcgct tcatacgggt caatcattag aacagattga acgtgatacc
    12481 gagcgcgatc gcttcctttc cgcccctgaa gcggtggaat acggtctggt cgattcgatt
    12541 ctgacccatc gtaattgatg ccagaggcgc aactgtgccg ctatacttat ccagggcggc
    12601 acaacgctgt aagcggcttg cgcctgagaa tggcatttgc gtcgtcgtgt gcggcacaaa
    12661 gaacaaagaa gaggttttga cccatgacag ataaacgcaa agatggctca ggcaaattgc
    12721 tgtattgctc tttttgcggc aaaagccagc atgaagtgcg caagctgatt gccggtccat
    12781 ccgtgtatat ctgcgacgaa tgtgttgatt tatgtaacga catcattcgc gaagagatta
    12841 aagaagttgc accgcatcgt gaacgcagtg cgctaccgac gccgcatgaa attcgcaacc
    12901 acctggacga ttacgttatc ggccaggaac aggcgaaaaa agtgctggcg gtcgcggtat
    12961 acaaccatta caaacgtctg cgcaacggcg ataccagcaa tggtgtcgag ttgggcaaaa
    13021 gtaacattct gctgatcggt ccgaccggtt ccggtaaaac gctgctggct gaaacgctgg
    13081 cgcgcctgct ggacgtcccg ttcaccatgg ccgacgcaac cacgctgacc gaagccggtt
    13141 atgtgggcga agacgttgaa aacatcattc agaagctgtt gcagaagtgc gattacgacg
    13201 tacagaaagc gcagcgcggg attgtctaca tcgatgaaat cgacaagatt tctcgtaagt
    13261 cagacaaccc gtccattacc cgagacgttt ccggtgaagg cgtacagcag gcactgttga
    13321 aactgatcga aggtacggta gctgctgttc caccgcaagg tgggcgtaaa catccgcagc
    13381 aggaattctt gcaggttgat acctctaaga tcctgtttat ttgtggcggt gcgtttgccg
    13441 gtctggataa agtgatttcc caccgtgtag aaaccggctc cggcattggt tttggcgcga
    13501 cggtaaaagc gaagtccgac aaagcaagcg aaggcgagct gctggcgcag gttgaaccgg
    13561 aagatctgat caagtttggt cttatccctg agtttattgg tcgtctgccg gttgtcgcaa
    13621 cgttgaatga actgagcgaa gaagctctga ttcagatcct caaagagccg aaaaacgccc
    13681 tgaccaagca gtatcaggcg ctgtttaatc tggaaggcgt ggatctggaa ttccgtgacg
    13741 aggcgctgga tgctatcgct aagaaagcga tggcgcgtaa aaccggtgcc cgtggcctgc
    13801 gttccatcgt agaagccgca ctgctcgata ccatgtacga tctgccgtcc atggaagatg
    13861 tcgaaaaagt ggttatcgac gagtcggtaa ttgatggtca aagcaaaccg ttgctgattt
    13921 atggtaagcc ggaagcgcaa caggcatctg gtgaataatt aaccattccc atacaattag
    13981 ttaaccaaaa aggggggatt ttatctcccc tttaattttt cctctattct cggcgttgaa
    14041 tgtgggggaa acatccccat atactgacgt acatgttaat agatggcgtg aagcacagtc
    14101 gtgtcatctg attacctggc ggaaattaaa ctaagagaga gctctatgaa tcctgagcgt
    14161 tctgaacgca ttgaaatccc cgtattgccg ctgcgcgatg tggtggttta tccgcacatg
    14221 gtcatcccct tatttgtcgg gcgggaaaaa tctatccgtt gtctggaagc ggcgatggac
    14281 catgataaaa aaattatgct ggtcgcgcag aaagaagctt caacggatga gccgggtgta
    14341 aacgatcttt tcaccgtcgg gaccgtggcc tctatattgc agatgctgaa actgcctgac
    14401 ggcaccgtca aagtgctggt cgaggggtta cagcgcgcgc gtatttctgc gctctctgac
    14461 aatggcgaac acttttctgc gaaggcggag tatctggagt cgccgaccat tgatgagcgg
    14521 gaacaggaag tgctggtgcg tactgcaatc agccagttcg aaggctacat caagctgaac
    14581 aaaaaaatcc caccagaagt gctgacgtcg ctgaatagca tcgacgatcc ggcgcgtctg
    14641 gcggatacca ttgctgcaca tatgccgctg aaactggctg acaaacagtc cgttctggag
    14701 atgtccgacg ttaacgaacg tctggaatat ctgatggcaa tgatggaatc ggaaatcgat
    14761 ctgctgcagg ttgagaaacg cattcgcaac cgcgttaaaa agcagatgga gaaatcccag
    14821 cgtgagtact atctgaacga gcaaatgaaa gctattcaga aagaactcgg tgaaatggac
    14881 gacgcgccgg acgaaaacga agccctgaag cgcaaaatcg acgcggcgaa gatgccgaaa
    14941 gaggcaaaag agaaagcgga agcagagttg cagaagctga aaatgatgtc tccgatgtcg
    15001 gcagaagcga ccgtagtgcg tggttatatc gactggatgg tacaggtacc gtggaatgcg
    15061 cgcagcaagg tcaaaaaaga cctgcgtcag gcgcaggaaa tccttgatac cgaccattat
    15121 ggtctggagc gcgtgaaaga tcgcatcctt gagtaccttg cggttcaaag ccgtgtcaac
    15181 aaaatcaagg gaccgatcct ctgcctggta gggccgccgg gggtaggtaa aacctctctt
    15241 ggtcagtcca ttgccaaagc caccgggcgt aaatatgtcc gtatggcgct gggcggcgtg
    15301 cgtgatgaag cggaaatccg tggtcaccgc cgtacttaca tcggttctat gccgggtaaa
    15361 ctgatccaga aaatggcgaa agtgggcgtg aaaaacccgc tgttcctgct cgatgagatc
    15421 gacaaaatgt cttctgacat gcgaggcgat ccggcctctg cactgcttga agtgctggat
    15481 ccagagcaga acgtagcgtt cagcgaccac tacctggaag tggattacga tctcagcgac
    15541 gtgatgtttg tcgcgacgtc gaactccatg aacattccgg caccgctgct ggatcgtatg
    15601 gaagtgattc gcctctccgg ttataccgaa gatgaaaaac tgaacatcgc caaacgtcac
    15661 ctgctgccga agcagattga acgtaatgca ctgaaaaaag gtgagctgac cgtcgacgat
    15721 agcgccatta tcggcattat tcgttactac acccgtgagg cgggcgtgcg tggtctggag
    15781 cgtgaaatct ccaaactgtg ccgcaaagcg gttaagcagt tactgctcga taagtcatta
    15841 aaacatatcg aaattaacgg cgataacctg catgactacc tcggtgttca gcgtttcgac
    15901 tatggtcgcg ctgataacga aaaccgtgtc ggtcaggtaa ccggtctggc gtggacggaa
    15961 gtgggcggtg acttgctgac cattgaaacc gcatgtgttc cgggtaaagg caaactgacc
    16021 tataccggtt cgctcggcga agtgatgcag gagtctattc aggcggcgtt aacggtggtt
    16081 cgtgcgcgtg cggaaaaact ggggatcaac cctgattttt acgaaaaacg tgacatccac
    16141 gtccacgtac cggaaggtgc gacgccgaaa gatggtccga gtgccggtat tgctatgtgc
    16201 accgcgctgg tttcttgcct gaccggtaac ccggttcgtg ccgatgtggc aatgaccggt
    16261 gagatcactc tgcgtggtca ggtactgccg atcggtggtt tgaaagaaaa actcctggca
    16321 gcgcatcgcg gcgggattaa aacagtgcta attccgttcg aaaataaacg cgatctggaa
    16381 gagattcctg acaacgtaat tgccgatctg gacattcatc ctgtgaagcg cattgaggaa
    16441 gttctgactc tggcgctgca aaatgaaccg tctggcatgc aggttgtgac tgcaaaatag
    16501 tgacctcgcg caaaatgcac taataaaaac agggctggca ggctaattcg ggcttgccag
    16561 cctttttttg tctcgctaag ttagatggcg gatcgggctt gcccttatta aggggtgttg
    16621 taaggggatg gctggcctga tataactgct gcgcgttcgt accttgaagg attcaagtgc
    16681 gatataaatt ataaagagga agagaagagt gaataaatct caattgatcg acaagattgc
    16741 tgcaggggct gatatctcta aagctgcggc tggccgtgcg ttagatgcta ttattgcttc
    16801 cgtaactgaa tctctgaaag aaggggatga tgtagcactg gtaggttttg gtacttttgc
    16861 cgttaaagag cgtgctgccc gtactggccg caacccgcag accggtaaag agatcaccat
    16921 cgctgctgct aaagtaccga gcttccgtgc aggtaaagca ctgaaagacg cggtaaacta
    16981 agcgttgtcc ccggtgggga tgtgacgaag ttcaagggcg catctactga tgtgcctttt
    17041 ttatttgtat tcggtgactt tctgcgtctt gtgggctgac aattgccccc gtttcttgtc
    17101 acaataggcc tttgcgcgca tcgatacgtt gcgtgaggta cacagtcatc tacagcggag
    17161 tgttgttaca ccatgatgga cagcttacgc acggctgcaa acagtctcgt gctcaagatt
    17221 attttcggta tcattatcgt gtcgttcata ttgaccggcg tgagtggtta cctgattggc
    17281 ggaggcaata actacgccgc aaaagtgaat gaccaggaaa tcagccgtgg gcagttcgag
    17341 aacgccttta acagcgagcg taatcgcatg cagcaacagc tgggcgatca atactccgag
    17401 ctggcagcga acgaaggcta tatgaaaacc ctgcgtcaac aggtgctgaa tcgtctgatc
    17461 gacgaggcgc tgttggatca gtacgctcgt gagctgaaac tgggtatcag tgatgagcag
    17521 gttaaacagg cgattttcgc gaccccagcc ttccaggttg acggcaaatt tgataacagc
    17581 cgctataacg gtatcctcaa ccagatgggg atgaccgccg atcagtacgc ccaggcgctg
    17641 cgtaaccagc tcactaccca acagctgatt aacggtgttg ccggtaccga ttttatgctg
    17701 aaaggtgaaa ccgacgagct ggcggcactg gtcgcgcaac aacgcgtggt gcgtgaggcg
    17761 actatcgatg ttaacgcgct ggcggcgaag cagcctgtga ccgaacagga gattgccagc
    17821 tactacgaac aaaacaaaaa caatttcatg acgccggaac aattccgcgt gagttacatc
    17881 aagctggatg ccgcaacgat gcagcaaccg gttagcgatg cggatatcca gagctactac
    17941 gatcagcatc aggatcaatt cacccagccg cagcgtaccc gctacagcat catccagacc
    18001 aaaactgaag atgaagcgaa agcggtactt gatgagctga ataaaggcgg tgattttgct
    18061 gcattagcca aagaaaaatc tgccgatatt atctctgccc gtaacggcgg cgatatgggt
    18121 tggttagaag atgccactat ccccgacgaa ctgaaaaatg ctggtctgaa agaaaaaggg
    18181 caactgtctg gtgtcatcaa atcttcggtc ggtttcctga ttgtacgtct ggacgacatt
    18241 cagccagcga aagtgaaatc gttagacgaa gtacgtgacg acattgcggc gaaagtgaaa
    18301 cacgaaaaag ccctcgatgc gtactatgcg ctgcagcaga aagtgagcga tgcggcaagc
    18361 aacgacaccg agtctctggc cggtgcagag caagctgccg gcgttaaagc cactcagacg
    18421 ggttggttca gcaaagataa cctgccggaa gagttgaact tcaagccggt tgccgacgct
    18481 atctttaacg gcggtctggt aggtgaaaac ggcgcgccgg gcatcaactc tgacatcatc
    18541 accgtagacg gcgaccgcgc attcgtgctg cgcatcagcg agcacaaacc ggaagcggtg
    18601 aaaccgttgg cagatgttca ggaacaagtt aaggcattgg ttcagcacaa caaagctgaa
    18661 caacaggcga aagtggatgc tgagaaactg ctggttgatt tgaaagccgg caaaggtgcg
    18721 gaagctatgc aggctgccgg tctgaaattt ggcgagccga aaaccttaag ccgttccggt
    18781 cgtgacccga ttagccaggc ggcgtttgca ctgccactgc cagcgaaaga caaaccgagc
    18841 tacggtatgg cgaccgatat gcaaggtaat gtggttctgc tggcgctgga tgaagtgaaa
    18901 caaggttcaa tgccggaaga tcagaaaaaa gcgatggtgc agggtatcac ccagaacaac
    18961 gcacaaatcg tctttgaagc tctgatgagt aacctgcgta aagaggcgaa aatcaaaatt
    19021 ggcgatgcgc tggaacagca ataatcctga agccgcctcg caaaaaaatg cgttgcagct
    19081 gtaacaagaa aaggtcgctt tcgcggcctt ttccatttct gaacattgcc atttgtttac
    19141 tgttttcact gccgttaagg tgattccact gttaacaaac aaggagaaaa cagtatgaaa
    19201 cacggaatta aagcactgct cattaccctg tccctggcct gtgccggaat gtctcatagc
    19261 gcgctggcgg cagcttctgt ggcgaaaccg acaacggtag aaaccaaagc ggaagctcct
    19321 gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg acgaagaagg cacccgggtc
    19381 agcattaata atgccagcgc ggaagagcta gcccgcgcga tgaatggcgt tggcctgaag
    19441 aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc cgtttaaaac ggtggaggat
    19501 ctaaagcagg tgccggggat gggcaattcg ctggtggaac gtaatctggc ggtattaacc
    19561 ctgtaattaa tttgcatagt ggcaattttt tgccagactg aagaggtcat accagttatg
    19621 acctctgtac ttataacaac aacgtaaggt tattgcgcta tgcaaacaca aatcaaagtt
    19681 cgtggatatc atctcgacgt ttaccagcac gtcaacaacg cccgctacct tgaatttctc
    19741 gaagaagccc gctgggatgg gttggaaaat agcgacagtt ttcagtggat gacggcccat
    19801 aacatcgcct tcgtcgtggt caatatcaat attaactatc gtcgcccagc ggtattaagt
    19861 gacctgttaa ctattaccag tcagttgcag caattaaacg gtaaaagcgg catcttaagc
    19921 caggtcatta cactggagcc ggaagggcag gtggtagcgg atgcgcttat tacgtttgtt
    19981 tgtattgatc ttaaaacgca gaaagcatta gctctggaag gggaattgcg cgaaaagctg
    20041 gagcagatgg ttaagtaaac gttttgtggt gccggatgct caagccgcat ccggcgacac
    20101 ccggaataat tacctcaacc cggttttctg cttcatcgct gccatcaccg tcggtttatc
    20161 ggccagataa tgattcaaac cgttggcgcg taaattacat gccgcacaat gaccgcaacc
    20221 gtcgccttta atgccgttat agcaggtcaa cgtttcgtta cggactaaat ccagtttgcc
    20281 gtaataatct gccagcgccc aggtttccgc tttatcaatc cacatcagcg gcgtttcaaa
    20341 acgaatatct ttcgccatgc ccaaactgac ggcatggttt agtgctttca caaactcatc
    20401 gcggcaatcc gggtagccgg agaaatccgt ttcacagacg ccggtaatta cggcttctgc
    20461 ttttacctga tacgcatata ttgccgccag cgtcaggaac aaaatattac gcccagggac
    20521 aaacgtattc gggatgccat cggcttcagg ttcataatca ggcaccggaa tgctgtcacg
    20581 tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca tcaagaacct tatgcgcgcg
    20641 tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt tctgcgcgat gtcgctgacc
    20701 gtaatcgaac gtcacgcaat ggacttcatc atattgttgt aatgcctgca ccagacaggt
    20761 ggtggaatcc tggcctccac tgaacacaac gacagcacgt ttcatagata atcctgcttg
    20821 aacaataaaa gcgttatggt aacgcctgcg attaacccgg accagctatt cattgcgacg
    20881 gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg cggtgtcagt cgcacaccgt
    20941 tcacgccggg aggggcacta atgcgatagt gatagttgaa cagcggcgtc agcgtcgcgt
    21001 ctgccattaa ctggctaaaa accgctttca gggcattaaa tcggttttcc tcatcaggca
    21061 ttacttgcac cgcgtccagc gtcgattgca gatgtgcata tgctggagcg tcgaaaacat
    21121 gtggccatag tggatcgcaa cgtagccatt gctccagagt atattccggt gcttcgccaa
    21181 ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag ggtcgtgtcg tcccagtttt
    21241 ttgcgttatg aaaaataatt gtgagttcac agccttccgc tgccagtgtc gcctgtaggc
    21301 gttctgccat ggtatgaagt tctatcggta ggtgataaac cagcgtcaag gttttcggta
    21361 gtttgacttc atccggtact tgccagtgcg gaatagtcca gcctggcagt aatgcatgac
    21421 tggcggtgat caggttttct ccgacttcta acgtttgtaa taaaccggat tgatgaataa
    21481 tggagatcac ttttcgcgcc tgccagaggg agagtcgggg acttttgcgc aacgttaaat
    21541 agcaaaaacc taaactgatg ccactactga cctggctgac ccgttgcagc tcctccggtt
    21601 tgccgatggt gatttgcacg ggatgccgac aactggttcc caaatctttt tcgaaaagcg
    21661 gcggagttat ccagtactca accgctttaa gcagcggatg acgcaaatgg taataatcat
    21721 ggctctccag gcgcaccagc tccgctgtga attgtgttaa gcgaaaagga ccagtgccga
    21781 tcagtgggaa ttgcggatgc gccagatggc tgcaatagct cgccagccgg tgcgcaagcc
    21841 agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg atgggtgact tcaatacgct
    21901 tcacgctaat aaataattga tccagtgctg gcagttgtaa cagcatcaat aatcgctggt
    21961 gtaagtgtga ggcttttact gcatcgccgt tatgccagtg tagggttgaa cgaagataaa
    22021 agtcccagcg taacccgtca gtagaggttt cccagtgatg cgctaaatcg ccaatcgggc
    22081 gctgagtatt attatcgaag cgggtcaggc cggaaaatat ctgcccggcg agatgctgct
    22141 cggcacggcc gggcaaaaag cctggttgta gcggttcgag cgggcgatag tagggaatac
    22201 gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa cggctgtaac agagtgcgca
    22261 gctcacctgg ggccagttgc gccagctcca gcacatcttg ctgctttccg gtttccagtg
    22321 cctgttccat catcgcattg cgtagcgatt ccggcgtgac cagaaagcgt aattgtccgc
    22381 gctttccgcg tcctgactgc gcctgccact ccagccatcc cgcctcctgt gcctgacgca
    22441 acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc ggccagttcg ctgacggtga
    22501 cggtttgcgg ctttccggct gaaggttgcc acagacgttg atactggtta agacggttga
    22561 gcaatcgcat ataaacccgg aacaatatta tttaactatt cactattact tccgtatata
    22621 tcaggtgata ctcaatcacc attaaccgtg tcacagagtg gagaaagaaa tggctcgtct
    22681 ggcagcattt gatatggatg gcactttatt gatgcctgac catcatttag gtgagaaaac
    22741 cctctctact ttggcgcgac tgcgtgaacg cgacattacc ctcacttttg ccacggggcg
    22801 tcatgcgctg gagatgcagc atattctcgg ggcgctatcg ctggatgcat atttgattac
    22861 cggcaacgga acgcgcgtgc attctctgga aggtgaactt ttacatcgtg atgatttacc
    22921 tgcggatgtc gcggagctgg tgctgtatca gcaatgggat acccgagcca gcatgcatat
    22981 cttcaatgac gacggttggt ttaccgggaa agagatccct gcgttgttgc aggcatttgt
    23041 ctatagcggt tttcgttatc agataatcga tgtcaaaaaa atgccactcg gcagcgtcac
    23101 caagatctgc ttctgtggcg atcacgacga tcttacacgc ttgcagatcc agctatacga
    23161 agcattaggc gagcgtgcac atttgtgttt ttccgccacg gattgcctcg aagtgctgcc
    23221 ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc caacatttag gtttatcgtt
    23281 gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc gaaatgttag gcagcgtcgg
    23341 tagcgggttt attatgggca atgcgatgcc gcaactgcgc gcggagctcc cgcatttacc
    23401 ggtgattgga cattgccgaa atcaggctgt ctctcactat ttgacgcact ggctggacta
    23461 tccacatcta ccttattccc ccgaataacg agatcccttc cagcaccggg caattgcccg
    23521 gttttttttg cgttgaattt gtcattttgt gccgtggtgt ttaaaccgca cagaataaat
    23581 tgtcgtgatt tcacctttaa aatagaatta aaagagaaaa aaattctctg tggaagggct
    23641 atgttagata aaattgaccg taagctgctg gccttactgc agcaggattg caccctctct
    23701 ttgcaggcac tggctgaagc cgttaatctg acaaccaccc cttgctggaa gcgcctgaaa
    23761 cggctggagg acgacggtat ccttatcggc aaagtcgccc tgctggatcc ggaaaaaata
    23821 ggcctcggcc tgaccgcttt tgtgctgata aaaacgcaac atcacagcag cgaatggtat
    23881 tgccgctttg tcacggtggt taccgaaatg ccagaagtgc tggggttctg gcgcatggct
    23941 ggtgaatacg attatctgat gcgcgtccag gttgccgaca tgaaacgcta cgacgagttt
    24001 tataagcgtc tggtaaacag cgtgccgggg ctgtcggacg tcacttccag cttcgcgatg
    24061 gaacagatta aatacaccac ttctttaccc atcgaataaa tatccagaat caggtcagga
    24121 cacaacgcgt gcgattattt gctcaattaa gctggtattt ccgtcgggaa tggcgtcgct
    24181 atctcggggc tgtcgccttg cttgtcatta tcgcgatgct gcaactggtt ccgccaaaag
    24241 tggttggtat tgttgtcgat ggcgtgacag aacaacactt tactaccggg cagatcctga
    24301 tgtggatcgc caccatggtg ctgattgccg ttgtggttta tctcctgcgt tacgtctggc
    24361 gggtattgct gtttggtgcg tcttatcaac tggctgttga actgcgtgaa gattattacc
    24421 gtcagctaag ccggcagcat cctgagtttt acctgcgtca tcgcacgggt gatctcatgg
    24481 ctcgtgcgac caatgacgtc gatcgcgtcg tgtttgccgc cggagaaggg gtgctgacgc
    24541 tggtggattc actggtgatg ggctgcgctg tgttgattat gatgtctacg caaattagct
    24601 ggcagttgac cttatttgcc ctgttgccga tgccagtgat ggcgatcatg atcaagcgca
    24661 acggcgatgc cttgcatgaa cgctttaagc tggcacaggc ggcgttttct agtcttaatg
    24721 accgcaccca ggaaagcctc accagtatcc gcatgatcaa agcctttggt ctggaagatc
    24781 gccagtcggc gttatttgcc gcggatgccg aagataccgg caaaaaaaac atgcgggtgg
    24841 cgcgtattga tgctcgtttc gacccaacca tctatatcgc gattggtatg gcgaacttgc
    24901 tggcgattgg cggcggtagt tggatggtgg cgcagggcag tttaacgctg ggccagctca
    24961 ccagttttat gatgtattta ggtctgatga tttggccaat gctggcgctg gcatggatgt
    25021 ttaacattgt ggaacgtggt agtgctgcgt acagccgtat tcgtgcgatg ctggcggaag
    25081 cgccggtggt gaacgatggt agcgaacccg tgccggaagg gcgtggcgaa cttgatgtaa
    25141 atattcacca gttcacgtat ccgcagactg accatcctgc gctggaaaac gtcaatttcg
    25201 ccctgaaacc cggtcagatg ctgggtatct gcgggccgac tggttccggc aaaagtaccc
    25261 tgttgtcgct cattcagcgt catttcgacg tcagcgaggg ggatattcgc tttcatgata
    25321 ttcctctgac gaagttacaa ctcgatagct ggcgtagccg cctggcggta gttagccaga
    25381 caccgttcct tttttccgac accgtagcga acaacatcgc gttgggttgc ccgaatgcca
    25441 cccagcaaga gattgagcat gtcgcgcggt tagccagcgt acatgacgat attttgcgtc
    25501 taccgcaagg ttacgataca gaagtgggcg agcgcggcgt gatgctttcc ggtgggcaaa
    25561 aacagcgtat ctccattgct cgagcgttat tagtcaacgc ggaaatcctc atccttgatg
    25621 atgcgctttc ggcggtggac ggacgcacag agcaccagat cctgcataac ctgcgtcagt
    25681 ggggacaggg aagaacggta atcatcagtg cccatcgcct ttcggcactg acggaagcca
    25741 gtgaaattat tgtgatgcag cacggacata tcgcccagcg tggcaatcat gatgagctgg
    25801 cgcaacaaag cggctggtat cgcgatatgt atcgctatca acaactggag gcggcgctcg
    25861 acgacgctcc ggaaattcgc gaggaggccg tcgatgcgta gttttagcca actgtggccg
    25921 actctcaagc gtctgttagc gtacggttcg ccgtggcgta aaccgctggg gattgcggtc
    25981 ctgatgatgt gggttgcggc agcggcagaa gtcagtgggc cgctgcttat cagctatttt
    26041 atcgacaata tggtagcgaa aaataacctg ccgttgaaaa tggttgcagg gctggctgcg
    26101 gcgtatgttg ggctacaact gtttgccgcc gggctacatt acgcgcagtc gctgctgttt
    26161 aatcgggcgg cagtaggcgt agtgcaacag ttgcgtaccg acgtgatgga tgccgcattg
    26221 cgccagccat taagcgagtt tgatacccaa cccgtcgggc aggtgatttc ccgcgtcact
    26281 aatgacactg aagtgatccg cgatctctac gttaccgtag tggcaactgt cctgcgtagc
    26341 gccgcactgg tgggcgcgat gctggtggcg atgttcagcc tcgactggcg aatggcgctg
    26401 gtggcgataa tgattttccc ggtggtgctg gtggtaatgg tgatatacca gcgttacagc
    26461 acgccgattg tccgtcgtgt gcgcgcctat ctagcggata tcaacgacgg ctttaacgaa
    26521 atcatcaatg gcatgagcgt tatccagcag ttccgtcagc aggcgcgatt tggcgaacgt
    26581 atgggggagg ccagtcgttc acactatatg gccaggatgc aaaccctgcg cctcgacggt
    26641 tttctgctgc gtccgctgct gagtctgttt tcatcgctca ttctttgtgg cttgttgatg
    26701 ctgtttggct tctccgctag cggcaccatt gaagtgggcg tgctgtatgc gtttatcagc
    26761 tatcttgggc gacttaacga accattaatc gaactgacca cgcaacaggc gatgctgcaa
    26821 caggctgttg ttgctggtga gcgcgtgttt gaactgatgg acggaccgcg ccagcaatat
    26881 ggcaatgatg atcgcccgtt acagagtggc accatcgaag tcgataacgt gtcatttgct
    26941 tatcgcgatg acaatctggt gctaaagaac attaatctct ctgtgccttc gcgtaacttt
    27001 gtggcactgg tcgggcatac cggcagtggc aaaagcaccc tcgccagttt attgatgggc
    27061 tattacccgc taacggaagg tgagattcgc cttgatggtc gtccattaag ttcgctaagt
    27121 cacagcgcgc tgcgccaggg cgtggcaatg gtgcagcaag atccggtggt gctggcggat
    27181 accttcctcg ccaacgtgac gctggggcgt gatatctccg aagaacgcgt ctggcaggcg
    27241 ctggaaaccg tgcaactggc ggagctggcg cgcagcatga gtgatggtat ttatacgccg
    27301 ctgggcgagc aggggaataa tctctccgtc gggcaaaagc aactgctggc actggcacgc
    27361 gtgttggtgg agacgccgca aatcctgatc cttgatgagg caaccgccag cattgactcc
    27421 ggtactgaac aggcgattca acatgctctg gcggctgtgc gtgaacatac cacgctggtg
    27481 gtgattgctc accgcttatc gaccattgtt gatgccgaca ccattctggt gcttcatcgt
    27541 gggcaagccg tggagcaggg cactcaccag caactgctgg cggcccaggg acgctactgg
    27601 cagatgtatc aactgcaact tgcgggcgaa gagctggcag ccagcgtgcg tgaagaagaa
    27661 tcattgagcg cctgaatagc gcaatatttc atcgttggtg caaaaatgta acgcactgtg
    27721 cactgtcata gtgcgttttc attttcaatc ttcttaactt cctgctctct ttctcgtttt
    27781 tcatttctgg cacaccgctt gcaatacctt cttcgtgtag cagaaccatt accgaattct
    27841 gaccggaggg gatctatgaa gctggtgacc gtgataatca aaccattcaa gctggaagac
    27901 gttcgtgaag cgttatcttc cattggtatt cagggcctga ccgtcaccga agtgaaaggt
    27961 ttcgggcgtc agaaagggca tgccgagctg taccgggggg cggaatacag cgtcaacttc
    28021 ctgccaaaag taaaaattga tgtggcgatt gctgatgacc aactcgatga agtgatcgat
    28081 atcgtcagta aggcggctta caccggaaaa attggcgacg gcaaaatctt cgtcgctgaa
    28141 ttgcaacgcg tcattcgtat tcgtaccggc gaagccgacg aagcggcgct gtaatctctg
    28201 gcacacagca acaggaacga aaaatgaaga tagcgacgat aaaaactggg cttgcttcac
    28261 tggcgatgct gccgggactg gtaatggctg cacctgcggt ggccgataaa gccgacaatg
    28321 cgtttatgat gatttgtact gcgctggtgc tgtttatgac tattccgggg attgccctgt
    28381 tttacggtgg gttgattcgc ggcaaaaacg tgctgtcgat gctgacgcag gtgacggtga
    28441 cgtttgcact ggtctgtatt ctctgggtgg tttacggtta ctcgctggcg tttggtgagg
    28501 gcaacaactt cttcggcaac attaactggt tgatgctaaa aaacatcgaa ctgacggcgg
    28561 tgatgggcag catttatcag tatatccacg tggcgtttca gggctcgttt gcctgcatta
    28621 ccgtcggctt gatcgttggg gcgctggcgg aacgaatccg cttctcagct gtgttgattt
    28681 tcgtggtggt atggctgacg ctctcttaca ttccgattgc gcatatggtg tggggcggtg
    28741 gtttgctggc ttctcacggt gcgctggatt tcgcgggtgg cacggtggtg catattaacg
    28801 ccgcaatcgc cggtctggtg ggcgcgtatc tgataggcaa acgcgtgggc ttcggcaaag
    28861 aggcgtttaa accgcacaac ctgccaatgg tcttcaccgg tactgccatt ctctatattg
    28921 gttggtttgg ctttaacgcc gggtcagcgg gcacggcgaa tgaaatcgcg gcactggcat
    28981 ttgtgaatac tgtggtcgca acggcggcgg caattcttgg ctggatcttc ggtgaatggg
    29041 cgctgcgtgg taagccttct ctgctgggag cgtgttctgg cgcgattgcc ggtctggtcg
    29101 gcgtgacgcc agcgtgtggc tacattgggg ttggcggcgc gttgattatc ggcgtggtag
    29161 ctggtctggc gggcttgtgg ggcgttacta tgctcaaacg cttgctgcgg gtggatgatc
    29221 cctgcgatgt cttcggtgtg cacggcgttt gtggcattgt cggctgtatc atgactggga
    29281 tttttgccgc cagttcgctg ggtggcgtgg gcttcgctga aggtgtgacg atgggccatc
    29341 agttgctggt acagctggaa agcatcgcca ttacgatcgt ctggtccggt gtggtggcat
    29401 ttattggcta caaactggcg gatctgacgg ttggtctgcg tgtaccggaa gagcaggagc
    29461 gagaagggct ggatgtcaac agtcacggcg agaatgccta taacgcgtaa caagcactgc
    29521 aaaaaacagc cggacggttt tcacctccgg ctattttttt aattgtgatt acgcatcacc
    29581 ccttcctgaa cggtcgaggc aaccagtacg ccgtcctggg tataaaactc accgcgcaca
    29641 aagccacgtg cgctggacgc cgaggtgctc tccacgctat acagcagcca ttcattcaaa
    29701 ttaaacgggc gatggaacca catggaatgg tcaatggtgg caatctgaat ccccggttcg
    29761 agaaaaccga tgccgtgcgg ctgtagagct accggcagga agttaagatc agaagcgtaa
    29821 ccgagcagat actgatgaac gcgcaggtca tccggcacgc taccatttgc gcggatccac
    29881 acctgacgat gtggttctgc gacgtgacct ttcagtgggt tatgaaactc caccggacgg
    29941 acttccagcg gacgatcgca gatgaattta tctttaagca ctggcggcag caggtgcgcc
    30001 agcgattggg cgatttgcgt ttccgaaggc aggccatcag gcgctggcgc ggacggcatt
    30061 gttttttgat gttcgaaacc cgcttctggt gcctggaaag aggccgtcat ataaaaaatc
    30121 ggtttgccgt tttgaatagc agcaacccgg cgggcgctga agctgttacc gtcacgcagc
    30181 gtttcgacat cataaataat cggcttctta ctatcgccag ggcgaagaaa gtagctgtga
    30241 aacgaatgta ccagccgctc ttcagggacg gtctcttttg cagcatacaa ggcctgaccc
    30301 acgacctggc cgccaaacac ctggcgtaaa cctaaatctt cactctggcc gcgaaagagt
    30361 ccttcctcaa ttttttccag atttaacaat gtcagtaaat tttttagcgc ctgactcata
    30421 taactctcca gtaacaaagc tgccgcagca agccaaagtg agttgagtat aacgcaaatt
    30481 tgctactggt ccgatgggtg caatggtctg aattacgggc taattacagg cagaaatgcg
    30541 tgatgtgtgc cacacttgtt gacgttacta ttttgttaac cactcttccg gcgaggaaag
    30601 ttagcccgct ggtgcattga taataaggag aaatgaatga aactcgtgca catggccagt
    30661 ggtttagcgg ttgcgattgc gttggcggct tgcgcagata aaagcgcgga tattcagacg
    30721 ccagccccgg ctgcaaatac gtctatttca gcaacacaac aacctgctat ccagcaaccg
    30781 aatgtctccg gtaccgtctg gatccggcag aaagtcgcac tgccgcctga tgctgtgctg
    30841 accgtgacac tttctgacgc gtcgttagcc gatgcaccgt caaaagtgct ggcgcagaaa
    30901 gcggtgcgta ccgaaggtaa acagtcacca ttcagctttg ttctgccatt taacccggca
    30961 gatgttcagc cgaacgcgcg tattctgttg agtgcggcga ttaccgtgaa tgacaaactg
    31021 gtatttatca ccgataccgt tcagccggtg atcaaccagg gcggaactaa agccgacctg
    31081 acattggtgc cggtacagca aaccgccgtg ccggttcagg ccagcggtgg cgcaacgact
    31141 accgtacctt cgacttcacc aactcaggtg aatccgtctt cggcagttcc agctcctacg
    31201 caatattaag cgacgttaac cctctccgcc cggagagggt tagtagttcc agcgataacg
    31261 ctgcaagtcg atttgcccgc ttcccgatac catcacacct tctgccagta atgcctgtcg
    31321 ctgacgctgt aaatccggtc cggttagcga aattgtgccg tggcgattaa ccacccggtg
    31381 ccagggtaag gtgctgcctt cagggagacg ctttaacaca ccgcccacct ggcgcgcggc
    31441 gcggggcgat cccgccagtt tcgccacatc accgtaagtg gtgacatagc cttcgggaat
    31501 agcggcgacg atttgccaga cgcgttgggg aaatgaatct tctttttcca tcttttcttc
    31561 ctgaggtaat ttttcagcat aatctggaaa aacgcccgag tgaagtcgca ttgcgcaaga
    31621 aaccagcatc tggcacgcga tgggttgcaa ttaaccgggg cagcagtgat aatgcgcctg
    31681 cgcgttggtt ctcaacgctc tcaatggggg ctctgttggt tctcccgcaa cgctactctg
    31741 tttaccaggt caggtccgga aggaagcagc caaggcagat gacgcgtgtg ccgggatgta
    31801 gctggcaggg cccccaccca tttctgcctc ccaccgtttc gtcaaaaaaa accaacatgg
    31861 ctaaacttta accacaactg acgtcgcaag aattgtctgg ctgcgcagta cgcttcggag
    31921 gtatgtctga tgaagtatgt tgatggtttt gtggttgccg ttcctgccga taaaaaggat
    31981 gcctatcggg aaatggccgc taaggctgcg ccattgttta aagagtttgg cgcgcttcgt
    32041 attgtcgaat gctgggccag cgatgtaccg gatggcaaag tgaccgattt tcgtatggcg
    32101 gtgaaagcgg aagagaatga agaggttgtc tttagctgga ttgaataccc ttcaaaagag
    32161 gtccgcgacg ctgctaatca aaagatgatg tcggacccac ggatgaaaga gttcggcgag
    32221 tccatgccgt ttgacggcaa gcgaatgatc tatggcggat tcgagtcaat catcgacgaa
    32281 tagttagcgt gacgggctgt gcggcgtgcc gtcagcccaa aaattcacaa atgttgctcg
    32341 gcccagcgta agaaatcttc tttcggtaat gccttgctgt agagccagcc ctgaccgtag
    32401 tgcacgccat gctggcgtag ccactcttct tgtttactgg tttcgattcc ctccgctacc
    32461 attttcagtt tcagtgtttt tgccatttcg atgatatgcg gcgtgacatt tttatattcc
    32521 agcgcatcaa cgaaagattt atcgatcttc agaatgtcga catccagatc ctgtaaataa
    32581 cttaaacttg aatatcccgt accaaaatca tcaagataaa tttcatggcc cgcctcccgg
    32641 tagcgagaaa ttatcggggc gctggttttc ggatcggcaa actcgcgttc agtgagttca
    32701 agcgcgatct gtctgggatt aacctgatag tgattgatca tttcacgcag caattgcggg
    32761 attttttccg aggtgagcac ggtggattca agattgatcg aaatatgctg ctgtggatgc
    32821 tgacgcagcc agtcgcccat atcttcaaag acgcttctta taatcagtag cgtcaatggc
    32881 tcagaaaggc ccgtttgctg agccagcgga ataaaactat ctggtgacaa ccaactaccg
    32941 tctgtctgcg gccagcgcgc cagtgcctca gcaccgacaa ttttgccatt ggctaaggag
    33001 acaatcggct gatagtgcac gcaaatatca cgattttcga tagcatcctg cagccgatga
    33061 tgcggtgact ggatacggcg caaaatacgc agcacaaaca tcgctgccag caggccaatc
    33121 accaacccga gcggtaacca aataaagact tgccgatgcc agcctttctg caacattttc
    33181 gttgaagccc aggtgatgat cgaaatattc atctccggga agggcaggat atcgtaaatg
    33241 attccattat tttcgatatg ctcaccgggt gttttttgta gcctggtaat aattccctga
    33301 gcaatttcat cgctgctggt tatgacaacg ttatgggcat tgccaataat ggcggcatca
    33361 atttgccatg agctataggg aatgacatca ataaaggaag cggggtcgat catgacgaca
    33421 taatgtgccg ttcccatggc gaccatgtaa cggataatgc ctaaatcgtt atgcgatgtt
    33481 aaccagacac gataaccatc tttcgaaatt ttacctggct cggggaaggt atcgggcgga
    33541 ctttcatgct ccagagacga acactgggga acgttgttat cgatataaac cacttcctga
    33601 atatagcgat aactgtaaga tacccgacgc atttccatga gatgggcttc gctacaggca
    33661 gcgccttgcc atctttccag ctcttgcagc gcatctttcc cttgtgtcgc caccttattg
    33721 gcccgaatag cgacgcggga ggaataggta tccagctctt caataaacga tgtttctacc
    33781 tgctgatggg ccagccagat gcttaagccg acaggcagca atactgaaag aatcagtact
    33841 cccgaaataa ggccgaccag atgtcgtgtt ctcaccagca tatccttatt aacacagtgt
    33901 agggaaagta tatctgatag tggaaaaagg ctggcaggag ataaggaggt agagctggta
    33961 tttcatgaag tgatgggtgg aaacgatcat tgtcatttga ccaggcgcag agctttacgc
    34021 ctggtatgta accctaactt aagatgttga ttcggcgcga gcgagggtaa aaatatcgta
    34081 aaaagacagt tcacctttac ggacgatcat tttttgcagt tgttccttat gaacatccgc
    34141 gacacgcgga gagttcaaga tggcttcaac cagcgcggca ggtacaaagc gcgtgttgta
    34201 ccattcgccg ttatagcaaa cgcggaaatc cagcacgtcg atatcttcat agcggtagcg
    34261 tgggtagaca tcgaaaaaga agaaaccatt aagcaggata aataatacaa ccagtagcca
    34321 gacagagcct gacagcgttt cggactgcaa catcaccgcc agggtggcaa aaaaagcaac
    34381 gtacataccg ataaacagcc ccggatgttt acggataaaa ctgatactaa agcggggttt
    34441 gttgtcgcgt ttttcgcggg tattcagaga ctcaatcgtt tcggtcagca ggcgttgtat
    34501 ttcggtcatc gtttgccttc gtggtatctg caactttcca gggaacaccc tattttaacg
    34561 tgggaatagt agagaggaag taacagattt accacaattt tgcataacag ttgcgaaaaa
    34621 ccgattacaa tttttttaat tattctggct ggattaccgc ccacgacaac gttgtccggg
    34681 acatcttttg tgacaactgc acctgaggct accacgacgt tatcaccaat ggtcacacca
    34741 gggttaatga ccgcgcgtcc gccaatccag acgttattac cgatggtgac gggtttcccc
    34801 agttcagcac cgctattacg tgctacaggg tcgatgggat gtgttgccgt gtagatatga
    34861 acgcctggtg ccaacataca gttatcaccg atgcgaatag ggcagacatc aagcatcacg
    34921 caatcgaagt tggcgaaaaa attattaccg agaaaaatgt tatagccata gtcacagcga
    34981 aacgttggct caatataagc ctctgtcacc tgaccgaata gatcagcgag aatttgctgg
    35041 cgtaatgtgt gctcttccgc ctgggaatga ttgtatcggt gaataagctg acgagcgcgc
    35101 aggcgatcgc gagataacgt ctcatctgcc gagcgataca actcaccagc aatcatcttt
    35161 tctttttctg tgctcattac tgacctccgg ttgctgaaaa tgcggcaaca cagtaagacg
    35221 aaaagtcagt gaagagaacg ttcccattac atctgtgtga taaagatcac atagggtatg
    35281 tcttcatggt gaaaagtata aaattcttaa taaacagccg gttatagctc cgaaagcgaa
    35341 ttatttatta gcgaataaat ttccatactg aggaagggat cttgtcgtat agtttattca
    35401 tggtcaattc ggcgaggcgg tgatctgcgg ctgagtaaaa taccgccagt tcattatctg
    35461 ataattcgta tttatttttc tcgataacac gctccagcgt gtcaattgtc tggcaacgac
    35521 gtaaacgcat taaataatcg gttttcgtta aaggtttttc ggacatactt ctacccatgg
    35581 ttgtaataat tctaacaaga taaactcgca ggattctctt tcgtcgcatt gacaaaacaa
    35641 cggaatagtc gattacctga cttccgccat ttctgaagat cctgcatatt aataccataa
    35701 ctactgaaca acataaaggt gtcatccaga tattcgtcga tctgctcaat gagcttattg
    35761 tcttcattat acttaatttt gtaattaagt gcgaaggtcg caatatgctc aatcagttca
    35821 ttcaactgga ggttgatcgc cgaggttggg tcgttaaccc agccatgatt gctttcttca
    35881 aggtttgcaa ggcagtcatg atacagggtt tcacagagaa acttaagctg tgcgatatca
    35941 tgtcttttgg gtgagtattc atccataacg cgtccccttc ttagcggttg aactaacgga
    36001 cacctttcgg gatggaaaaa acttactgac ctggacttgc cctctcgttt gttagcttaa
    36061 ctataagcca ctctttgcag gttttcatcg cattattacg aaaaattaca attaggaagg
    36121 aatcgggaca ggactcactt tttcatacta gcactaaccc gcagcaggta aaagcagttt
    36181 gctcgaactg ttcataacat ttcaggatta tcgccagatg aaattggtga cactaatacc
    36241 aggattgctc tgaatatgac gtaataaccg aggaatgaat aaagaattac cgcaaataat
    36301 taagaatagc ctctaaatga ttatggatta ttaaatctat tagcgctact taatataaat
    36361 taactaacaa tcagtatttt ttatgaattt tatccgtggt taatactggt tttcgtatga
    36421 gatcctgagt tggtggttca attactcctt aatgttcgta ggttatgcat aaaaaaggcc
    36481 gcttgcgcgg ccttagtgat tacacgttgt atcaatgatg atcgacagta tggctgtgct
    36541 cgatatcttc attcttgcgg ctaaagcggc ggcgaaccac cacaaagaat accggaacga
    36601 agaagattgc cagtaccgtt gcggtcacca tcccgcccat tacaccggta cctactgcgt
    36661 tctgcgcgcc ggaaccagca ccagtactga taaccagcgg cataacgccg aggataaacg
    36721 ccagcgaggt catcaggatc ggacgtaaac gcatccgcac tgcatcaagc gtcgcttcaa
    36781 tcagaccttt accttcttta tccatcaagt ctttggcgaa ttcgacgata agtatcgcgt
    36841 tcttcgccga caacccaatg gttgtgagca ggcctacctg gaagtaaacg tcattggtca
    36901 ggccacggaa ggtggcagcc agcaacgcac cgataacccc cagcggaacg accagcataa
    36961 cggagaacgg aatcgaccag ctctcgtaca gcgccgccag acacaggaat acgacaatca
    37021 acgaaatcgc gtacagtgaa ggtgcctggt tgccggagag gcgttcctga taggacatcc
    37081 ccgtccagtc atagccaaca ccggtaggca gtttgctcgc cagttgttcc atcagctcca
    37141 ttgcttcacc ggtactttta cccggtgccg cctggcctaa gatttccatg gatggcaggc
    37201 cattgtaacg ttccagacgc ggcgaaccgt actcccaacg agaagaggag aatgccgaga
    37261 acggcaccat ctgaccatca gcagcacgaa cataccagtc gccgatatca tccggcagca
    37321 tacggtattt cgcttctgac atgacgtaaa ctttcttcac acgaccgcgg tcgataaagt
    37381 cgttcacata gctaccgccc catgcagcgc ccagagtggt gttaatgtca ttgatagaaa
    37441 cacccagcgc ctgcgctttt tcctggtcga tatcaatctt aaactgcggg gtatcttcca
    37501 gaccgtttgg acgtacgctg gtcaacatat cagggtgctt cgctgcttct gcaagcaact
    37561 ggttacgcgc ctgagtcagt ttttcgtgac caaggccagc ctggtcaatc agctcaaagt
    37621 caaagccggt tgcagtaccc agttccacga ttgcgggcag gttaaaggcg aaaaccatcg
    37681 catctttgat ttgcgagaaa gcgcgtgttg cacgcatggt aatcgcttca actttgtttt
    37741 cttcgcccgg acgatcggcc cagtccttca aggaaacgaa cgcaataccg gtattctgac
    37801 cacgtcccgc aaagccgaag ccgttaacgg cgaacaccga ctcaacgttg tttttttctt
    37861 tggtcagata gtaatgcgtt acctcattga gcactttctg cgtacgttcc tgcgttgcac
    37921 ctgctggcag ctgaaccatg gtcataaata cgccctggtc ctcatctggc aagaaggagc
    37981 ttggcagacg cacgaacaga taggccatgc cgaccacgat gatcagatac agcaccaggt
    38041 aacgccccgt actgcgcaga ataccgccta cgctgtcggt gtagtggtgc gtgctcttct
    38101 cgaacatgcg gttaaaccag ccgaagaagc cttttttacc ttccccgtga tcgcctttgg
    38161 caatcggttt cagcatggtg gcacaaagag ccggagtcag gatcaacgct accagtaccg
    38221 acagcgccat tgctgaaaca atggtaatag agaactgacg atagatagca ccagtagaac
    38281 cgccaaagaa ggccatcggt acgaataccg ctgacagtac catcgcgata ccgaccagag
    38341 cgccctgaat ctgccccatc gacttacggg tagcttcttt tggcggcaaa ccttcttccg
    38401 ccataacacg ctccacgttt tctaccacaa cgatagcgtc atccaccaac aagccgatgg
    38461 cgagcaccat cccgaacatt gttagcgtgt ttatcgagaa gccaaaggcg gcaaggacgg
    38521 caaaggtccc gagcaatacc accggtacgg caatggtcgg aatcaacgtc gcgcggaagt
    38581 tctgcaggaa cagatacata accaggaaca cgaggatgat cgcttcgacc agcgttttaa
    38641 ccacttcgtg aatagagatt ttcacgaacg gcgtggtgtc gtacgggtaa acaattttca
    38701 gacccgacgg gaagaacggt tccatcttcg ccagttcagc acggattgcc gcagcggtat
    38761 ccagcgcgtt tgcaccggtc gccagcttga tccccagacc ggaagccggt tggccgttaa
    38821 actctgcgat gatgtcgtag ttctcaccac ccagctcaat cttcgccaca tcacgcagca
    38881 gtacgcggga accatcctga ttcactttca gcaggatttt gccgaactct tcagtagagg
    38941 tcagacgcgt ctgagcaata atagaggcgt taagctgttg gcctttcacc ggcggcgtac
    39001 caccgagctg acccgccgca acctgggcgt tctgcgcttt gatggcggta atgacatcaa
    39061 ccggcgttag ctggaatttg ttcagctcat tcgggttcat ccagatacgc atcgcgtact
    39121 gtgaaccgaa caactgaaca tcacccacgc ccgacgtacg gctgatggca tctttcatat
    39181 tcgccgctac gtagtcagag atatcctcct gcgtcatggt gccatcggtg ttgataacgc
    39241 cgacaaccat caggaagctg ctggatgatt tctcaacgct caccccttgc tgctgaactt
    39301 cttgcggcag caacggcatc gccagctgca gtttgttctg cacctgaacc tgcgcgatat
    39361 ccgcatcagt accagactca aaggtcaggg tgatctgcac ggtacccgtg gagtcactgt
    39421 tagaggacat gtacatcagg ttatcgatac cgttcatatt ctgttcgata acctgtgtca
    39481 ccgtgtcctg cactgttttc gcatcagcgc cggggtagga ggcggagatc gttactgccg
    39541 gcggtgcaat cgtaggatat tgcgccaccg gcagtttgag gatcgccagc ccccctgcca
    39601 acatgatgat aatggcgatc acccacgcaa aaatcgggcg atcgataaag aaattaggca
    39661 tgtcttaacg gctcctgttt aagttaagac ttggactgtt caggctgagc accgcttgcg
    39721 gcttgctggt tattatcagc ggtaacttct tgtgctttta cctggacacc aggacgcact
    39781 ttctgcagcc cacttattac tacgcgatcg cctgctttca gaccttctgt caccagccac
    39841 ttatcgccaa tagcctggct tgcaacgatc ggacgggttt ccactttgtc atccgcgcca
    39901 actaccagta cggtggcatc accacgcggc gtacgggtta cgccctgttg cgggactaaa
    39961 atagcgtttg gattaagccc ttcttccaga cgtgcgcgca cgaacatacc cggcagcaga
    40021 gtgtgatccg ggttcgggaa gatagcgcgt agggtgatag acccagtggt ctgatcaacg
    40081 gtaacgtcag agaattccag cgtaccgtcc tgcgggaact taatgccgtc actggtgatc
    40141 agcgacactt tggctttgcc gttctcttgt ttcagcgtgc cattcgccag ttcctgtttc
    40201 aggcgcagga agtcgttgct ggactgggtc acatcaacgt agatcggatc aagttgctgc
    40261 acggttgcca gcgcagtcgc ctgaccgttc tgtaccaatg cgccttccgt cacgttcgac
    40321 ttaccaatgc gaccgctaat cggagaggtg actttggtgt aagccagatt gatccgcgca
    40381 gtttcaacgg cagctttcgc cgcagttacc gcagcattcg cctgttgcgc atcagccaga
    40441 gcctgatcgt actcttgctt actgatgtac tgagtaccga gcagtttctg ataacgattc
    40501 accgtcaatt gcgcgatatt ggctgcagcc tgggctttcg ccagatcacc tttcgcactg
    40561 tcgtatgtcg cctgataggt cgcaggatca atctgataga gagagacacc agcttcgatg
    40621 tcgctacctt ctttgaaatt acgcttcagg ataatcccgc taacttgagg acgaacttct
    40681 gcgatccggt aggcactggt gcgacccgga agctcggttg tgatctgcag aggttcagtt
    40741 ttgactgtta ctacgccaac ggcgggcatc tgctggccac cttgttgggc ctgtttgtcg
    40801 tcacatcctg ttagggctaa gctgcctgag agcatcagaa cgaccgccag aggcgtaaac
    40861 cctctgtttt tgttcatatg taaacctcga gtgtccgatt tcaaattggt caatggtcaa
    40921 aagttaataa acccattgct gcgtttatat tatcgtcgtg ctatggtaca tacattcaca
    40981 aatgtatgta aatctaacgc ctgtaaattc acgaacatat ggcacgaaaa accaaacaag
    41041 aagcgcaaga aacgcgccaa cacatcctcg atgtggctct acgtcttttc tcacagcagg
    41101 gggtatcatc cacctcgctg ggcgagattg caaaagcagc tggcgttacg cgcggtgcaa
    41161 tctactggca ttttaaagac aagtcggatt tgttcagtga gatctgggaa ctgtcagaat
    41221 ccaatattgg tgaactagag cttgagtatc aggcaaaatt ccctggcgat ccactctcag
    41281 tattaagaga gatattaatt catgttcttg aatccacggt gacagaagaa cggcgtcgat
    41341 tattgatgga gattatattc cacaaatgcg aatttgtcgg agaaatggct gttgtgcaac
    41401 aggcacaacg taatctctgt ctggaaagtt atgaccgtat agaacaaacg ttaaaacatt
    41461 gtattgaagc gaaaatgttg cctgcggatt taatgacgcg tcgggcagca attattatgc
    41521 gcggctatat ttccggcctg atggaaaact ggctctttgc cccgcaatct tttgatctta
    41581 aaaaagaagc ccgcgattac gtcgccatct tactggagat gtatctcctg tgccccacgc
    41641 ttcgtaatcc tgccactaac gaataaccct gaatctgact ccaggatttt tcctggacat
    41701 tttcgtcgtt gctattctgg ttcactgcgt cgtgatattc ttgcggtttg actttttcag
    41761 gtcgttcttc aggttcagaa accttcattc atcatgacta tgttccagta ttacaaacga
    41821 tcacggcatt ttgttttttc agcatttatt gcttttgttt ttgtcttgtt atgccagaac
    41881 acggcgtttg cgcgggcgtc atcgaatggt gatctgccga caaaagcgga cctgcaggcg
    41941 caacttgact cactaaataa acaaaaagat ctttctgctc aggacaaact ggtgcagcag
    42001 gatctgacag atacattagc caccctcgat aaaatcgatc gcataaaaga agagacagtt
    42061 cagctacggc aaaaagtcgc tgaagcgccg gaaaaaatgc gccaggcgac cgcggcgtta
    42121 acagcactta gcgatgtcga taacgacgaa gaaacgcgca aaattctgag tacgctttcg
    42181 ttgcgccagc tggaaactcg cgttgcccag gcgctggacg atttgcaaaa cgcacaaaac
    42241 gatctggcgt cttataacag ccagctggtt tcgttacaga cgcagcccga acgcgtgcaa
    42301 aatgcgatgt ataacgcttc gcagcagctg caacaaattc gcagtcgtct ggatgggact
    42361 gatgtcggcg agactgcctt acgtcccagc cagaaagtgt taatgcaggc tcagcaggcg
    42421 ttgctgaatg cggagattga ccagcagcgt aaaagcctgg aagggaacac cgtcttgcag
    42481 gataccttgc aaaagcaacg tgattacgtg acggcgaaca gcgcccgtct ggagcaccag
    42541 ttgcagctgt tgcaagaagc ggtaaacagc aagcgcctga ctttaactga aaaaacggcg
    42601 caggaagccg tctccccgga tgaagccgcg cgtattcagg ctaatccgct ggtgaaacag
    42661 gaactggaaa ttaaccagca gttaagtcag cgtctgatta ccgcgactga aaacggtaat
    42721 cagttgatgc agcaaaacat taaagtcaaa aactggctgg agcgggcgct gcaatcggaa
    42781 cgcaatatta aagagcagat tgccgtcctg aagggcagcc tgctgttgtc tcgtatcctt
    42841 taccagcaac aacaaacgct gccctcggcg gatgaactgg aaaacatgac caaccgcatc
    42901 gcggatttgc gtctcgaaca gtttgaagtt aaccagcagc gtgatgcact cttccagagc
    42961 gatgcgttcg tcaacaaact ggaagaaggt cacaccaacg aagtcaacag cgaagttcac
    43021 gatgcgttat tgcaagtggt tgatatgcgt cgcgaattgc tggatcaact caacaaacag
    43081 ttgggtaacc agctgatgat ggccattaac ctgcaaatca accagcagca gttaatgagc
    43141 gtatcgaaaa acctgaaatc catcctgact cagcaaatct tttgggtgaa cagtaaccgt
    43201 ccaatggact gggactggat caaagcgttc ccgcaaagcc tgaaagatga atttaagtcg
    43261 atgaaaatca cggtgaactg ggaaaaagcc tggcctgccg tttttatcgc tttcctcgcc
    43321 ggtttgccgc tgctgttgat tgccgggctg atccactggc gtctgggctg gctgaaagcg
    43381 tatcaacaga aacaggcttc cgctgtgggt tccctgcgta acgatagcca gctcaacaca
    43441 ccaaaagcga tccttatcga cctgatccgc gcgctgccgg tgtgcctgat tattctcgcg
    43501 gttggcctga ttctgttgac catgcagctc aacatcagcg aactgctatg gtcgttcagt
    43561 aaaaaactgg cgattttctg gctggtgttt ggcctgtgct ggaaggtact ggagaaaaat
    43621 ggcgttgccg tgcgtcactt cggcatgccg gagcagcaga ccagccactg gcgtcggcaa
    43681 attgtccgca tcagtctcgc gttgctgcct atccatttct ggtctgtggt ggcagaactt
    43741 tccccgctgc atctgatgga tgatgtgctg gggcaggcga tgattttctt caacctgctg
    43801 ctgattgcct tcctggtatg gccaatgtgc cgcgaaagct ggcgtgataa agagtcgcac
    43861 accatgcgac tggtcaccat taccgtgctg tcgattatcc cgatcgcatt gatggtgctg
    43921 actgccacgg gttatttcta caccacgctg cgcctctccg ggcgttggat tgaaaccgtt
    43981 tatctggtga tcatctggaa cttgctctac cagacggtat tgcgcggctt aagcgtagcg
    44041 gcgcggcgta tcgcctggcg tcgtgcgctg gcgcgtcggc agaatctggt gaaagagggc
    44101 gcagaaggtg ccgaaccgcc ggaagaaccc accattgcac tggaacaggt taaccagcag
    44161 acgctgcgta ttaccatgtt gctgatgttt gcgctgttcg gtgtcatgtt ctgggcaatt
    44221 tggtccgatt tgatcaccgt gttcagctat ctcgacagca tcacgctctg gcattacaac
    44281 ggcactgaag ctggcgctgc ggtggtgaaa aacgtcacca tgggcagtct gttgtttgcg
    44341 attatcgcct caatggtggc ctgggcgttg attcgcaacc tgcctggttt actggaagtg
    44401 ctggtgctct cgcgactgaa tatgcgtcag ggcgcgtcgt atgccattac taccatcctt
    44461 aactacatca ttattgccgt tggtgcgatg acggtgttcg gatcgctggg cgtctcttgg
    44521 gataaactcc agtggctggc cgcagcatta tccgtaggtc ttggttttgg tttacaagaa
    44581 attttcggta acttcgtctc cggtttgatc attctgttcg aacgtccggt gcgtattggc
    44641 gatacggtaa ccattggtag cttctcgggg acggtaagta agatccgtat tcgtgcgaca
    44701 acgattaccg atttcgatcg caaagaagtg atcatcccga acaaagcgtt tgttaccgag
    44761 cgtctgatca actggtcgtt gactgacact actacgcgtc tggtgatccg tctcggcgtg
    44821 gcctatggct ccgatctgga aaaagtgcgt aaagtgttac tgaaggcggc gactgagcac
    44881 ccaagggtga tgcacgaacc aatgccggaa gtcttcttta cggcgtttgg tgccagcacg
    44941 ttggatcatg agctgcgtct gtatgtgcgt gaactgcgtg accgtagtcg tactgtcgat
    45001 gagctgaacc gtactatcga tcagctgtgc cgtgaaaacg acatcaacat tgcctttaac
    45061 cagcttgaag tgcatctgca caacgagaag ggcgatgagg tgacggaagt caaacgcgac
    45121 tacaaaggcg atgacccgac gccagcggta gggtaataac caaagggggc gacatttagt
    45181 tgccccgaga ttactgacaa agtgcgcgtt gttcatgccg gatgcggcgt gaccgcctta
    45241 tccggcctac gaaaccgcaa gaattcaatg tattgcagga gcg
//
LOCUS       DAZGSP010000034        44519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZGSP010000034 DAZGSP010000000
VERSION     DAZGSP010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.034"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1724
                     /gene="adeD"
                     /locus_tag="ACHK74_002009"
     CDS             <1..1724
                     /gene="adeD"
                     /locus_tag="ACHK74_002009"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFP0220833.1"
                     /translation="EYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRYI
                     AGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVIC
                     DPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAWR
                     DHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITAGIEN
                     CHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWEI
                     AHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSDA
                     RKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTPGKR
                     YRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRLAPACGLLGGFGLNEG
                     ALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGLMS
                     TDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKFAF
                     TTLEVTE"
     gene            complement(1769..3160)
                     /gene="uhpT"
                     /locus_tag="ACHK74_002010"
     CDS             complement(1769..3160)
                     /gene="uhpT"
                     /locus_tag="ACHK74_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFP0220834.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3298..4617)
                     /gene="uhpC"
                     /locus_tag="ACHK74_002011"
     CDS             complement(3298..4617)
                     /gene="uhpC"
                     /locus_tag="ACHK74_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFP0220835.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            complement(4627..6129)
                     /gene="uhpB"
                     /locus_tag="ACHK74_002012"
     CDS             complement(4627..6129)
                     /gene="uhpB"
                     /locus_tag="ACHK74_002012"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFP0220836.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLSLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6129..6719)
                     /gene="uhpA"
                     /locus_tag="ACHK74_002013"
     CDS             complement(6129..6719)
                     /gene="uhpA"
                     /locus_tag="ACHK74_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFP0220837.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6794..7084)
                     /gene="ilvN"
                     /locus_tag="ACHK74_002014"
     CDS             complement(6794..7084)
                     /gene="ilvN"
                     /locus_tag="ACHK74_002014"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP0220838.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7088..8776)
                     /gene="ilvB"
                     /locus_tag="ACHK74_002015"
     CDS             complement(7088..8776)
                     /gene="ilvB"
                     /locus_tag="ACHK74_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFP0220839.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANSDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8882..8980)
                     /gene="ivbL"
                     /locus_tag="ACHK74_002016"
     CDS             complement(8882..8980)
                     /gene="ivbL"
                     /locus_tag="ACHK74_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFP0220840.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            9545..9634
                     /gene="tisB"
                     /locus_tag="ACHK74_002017"
     CDS             9545..9634
                     /gene="tisB"
                     /locus_tag="ACHK74_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFP0220841.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9897..9971)
                     /gene="ysdE"
                     /locus_tag="ACHK74_002018"
     CDS             complement(9897..9971)
                     /gene="ysdE"
                     /locus_tag="ACHK74_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFP0220842.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            10053..11237
                     /gene="emrD"
                     /locus_tag="ACHK74_002019"
     CDS             10053..11237
                     /gene="emrD"
                     /locus_tag="ACHK74_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFP0220843.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11245..11742)
                     /gene="yidF"
                     /locus_tag="ACHK74_002020"
     CDS             complement(11245..11742)
                     /gene="yidF"
                     /locus_tag="ACHK74_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFP0220844.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11739..12101)
                     /gene="yidG"
                     /locus_tag="ACHK74_002021"
     CDS             complement(11739..12101)
                     /gene="yidG"
                     /locus_tag="ACHK74_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFP0220845.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12091..12438)
                     /gene="yidH"
                     /locus_tag="ACHK74_002022"
     CDS             complement(12091..12438)
                     /gene="yidH"
                     /locus_tag="ACHK74_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFP0220846.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12547..12996
                     /gene="yidI"
                     /locus_tag="ACHK74_002023"
     CDS             12547..12996
                     /gene="yidI"
                     /locus_tag="ACHK74_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFP0220847.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13043..14536)
                     /gene="yidJ"
                     /locus_tag="ACHK74_002024"
     CDS             complement(13043..14536)
                     /gene="yidJ"
                     /locus_tag="ACHK74_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP0220848.1"
                     /translation="MKRPNFLFIITDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTYPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(14533..16248)
                     /gene="yidK"
                     /locus_tag="ACHK74_002025"
     CDS             complement(14533..16248)
                     /gene="yidK"
                     /locus_tag="ACHK74_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFP0220849.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKIDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            16415..17308
                     /gene="yidL"
                     /locus_tag="ACHK74_002026"
     CDS             16415..17308
                     /gene="yidL"
                     /locus_tag="ACHK74_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP0220850.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(17305..18119)
                     /locus_tag="ACHK74_002027"
                     /pseudo
     CDS             complement(17305..18119)
                     /locus_tag="ACHK74_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18119..19735)
                     /locus_tag="ACHK74_002028"
     CDS             complement(18119..19735)
                     /locus_tag="ACHK74_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP0220851.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            20031..20747
                     /gene="yidP"
                     /locus_tag="ACHK74_002029"
     CDS             20031..20747
                     /gene="yidP"
                     /locus_tag="ACHK74_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP0220852.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(20744..22405)
                     /gene="yidE"
                     /locus_tag="ACHK74_002030"
     CDS             complement(20744..22405)
                     /gene="yidE"
                     /locus_tag="ACHK74_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFP0220853.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(22602..23030)
                     /gene="ibpB"
                     /locus_tag="ACHK74_002031"
     CDS             complement(22602..23030)
                     /gene="ibpB"
                     /locus_tag="ACHK74_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFP0220854.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(23142..23555)
                     /gene="ibpA"
                     /locus_tag="ACHK74_002032"
     CDS             complement(23142..23555)
                     /gene="ibpA"
                     /locus_tag="ACHK74_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFP0220855.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(23549..23621)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            23861..24193
                     /gene="yidQ"
                     /locus_tag="ACHK74_002033"
     CDS             23861..24193
                     /gene="yidQ"
                     /locus_tag="ACHK74_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP0220856.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(24195..25409)
                     /gene="yidR"
                     /locus_tag="ACHK74_002034"
     CDS             complement(24195..25409)
                     /gene="yidR"
                     /locus_tag="ACHK74_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFP0220857.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIAE
                     SGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPHDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            25510..26610
                     /gene="cbrA"
                     /locus_tag="ACHK74_002035"
     CDS             25510..26610
                     /gene="cbrA"
                     /locus_tag="ACHK74_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFP0220858.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHISARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNEITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVLLKQPEKSNAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(26571..27908)
                     /gene="dgoT"
                     /locus_tag="ACHK74_002036"
     CDS             complement(26571..27908)
                     /gene="dgoT"
                     /locus_tag="ACHK74_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0220859.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(27989..29137)
                     /gene="dgoD"
                     /locus_tag="ACHK74_002037"
     CDS             complement(27989..29137)
                     /gene="dgoD"
                     /locus_tag="ACHK74_002037"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFP0220860.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(29134..29751)
                     /gene="dgoA"
                     /locus_tag="ACHK74_002038"
     CDS             complement(29134..29751)
                     /gene="dgoA"
                     /locus_tag="ACHK74_002038"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFP0220861.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(29735..30613)
                     /gene="dgoK"
                     /locus_tag="ACHK74_002039"
     CDS             complement(29735..30613)
                     /gene="dgoK"
                     /locus_tag="ACHK74_002039"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFP0220862.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(30610..31299)
                     /gene="dgoR"
                     /locus_tag="ACHK74_002040"
     CDS             complement(30610..31299)
                     /gene="dgoR"
                     /locus_tag="ACHK74_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFP0220863.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            31577..32233
                     /gene="yidX"
                     /locus_tag="ACHK74_002041"
     CDS             31577..32233
                     /gene="yidX"
                     /locus_tag="ACHK74_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFP0220864.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(32279..33091)
                     /gene="yidA"
                     /locus_tag="ACHK74_002042"
     CDS             complement(32279..33091)
                     /gene="yidA"
                     /locus_tag="ACHK74_002042"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFP0220865.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(33206..33604)
                     /gene="yidB"
                     /locus_tag="ACHK74_002043"
     CDS             complement(33206..33604)
                     /gene="yidB"
                     /locus_tag="ACHK74_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFP0220866.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(33844..36258)
                     /gene="gyrB"
                     /locus_tag="ACHK74_002044"
     CDS             complement(33844..36258)
                     /gene="gyrB"
                     /locus_tag="ACHK74_002044"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFP0220867.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(36287..37360)
                     /gene="recF"
                     /locus_tag="ACHK74_002045"
     CDS             complement(36287..37360)
                     /gene="recF"
                     /locus_tag="ACHK74_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFP0220868.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(37360..38460)
                     /gene="dnaN"
                     /locus_tag="ACHK74_002046"
     CDS             complement(37360..38460)
                     /gene="dnaN"
                     /locus_tag="ACHK74_002046"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFP0220869.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(38465..39868)
                     /gene="dnaA"
                     /locus_tag="ACHK74_002047"
     CDS             complement(38465..39868)
                     /gene="dnaA"
                     /locus_tag="ACHK74_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFP0220870.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            40165..40245
                     /locus_tag="ACHK74_002048"
     CDS             40165..40245
                     /locus_tag="ACHK74_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220871.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            40475..40615
                     /gene="rpmH"
                     /locus_tag="ACHK74_002049"
     CDS             40475..40615
                     /gene="rpmH"
                     /locus_tag="ACHK74_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFP0220872.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            40632..40991
                     /gene="rnpA"
                     /locus_tag="ACHK74_002050"
     CDS             40632..40991
                     /gene="rnpA"
                     /locus_tag="ACHK74_002050"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP0220873.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            40955..41212
                     /gene="yidD"
                     /locus_tag="ACHK74_002051"
     CDS             40955..41212
                     /gene="yidD"
                     /locus_tag="ACHK74_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFP0220874.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            41215..42861
                     /gene="yidC"
                     /locus_tag="ACHK74_002052"
     CDS             41215..42861
                     /gene="yidC"
                     /locus_tag="ACHK74_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFP0220875.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            42967..44331
                     /gene="mnmE"
                     /locus_tag="ACHK74_002053"
     CDS             42967..44331
                     /gene="mnmE"
                     /locus_tag="ACHK74_002053"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFP0220876.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        10734 a        12085 c        11457 g        10243 t
ORIGIN      
        1 ctgaatacca ggaattgtta gccgtttccc gtggcgacgc tgttgccgat tatattattg
       61 ataatgtctc tattctcgac ctgatcaatg gcggagaaat ttccggccca attgtgatta
      121 aaggacgtta cattgccggt gttggcgcag aatacgctga tgctccggct ttgcagcgga
      181 ttgatgctcg cggcgcaacg gcggtgccag ggtttattga tgctcacctg catattgaat
      241 ccagcatgat gacgccggtc acttttgaaa ccgctaccct gccgcgcggc ctgacgaccg
      301 ttatttgcga ccctcatgaa atcgtcaacg tgatgggaga agccggattc gcctggtttg
      361 cccgctgtgc cgaacaggca aggcaaaacc agtacttaca ggtcagctct tgcgtacccg
      421 ccctggaagg ctgcgatgtt aacggtgcca gttttaccct tgaacagatg ctcgcctggc
      481 gggaccatcc gcaggttacc ggccttgcag aaatgatgga ctaccctggc gtaattagcg
      541 ggcagaatgc gctgctcgat aaactggatg catttcgcca cctgacgctg gacggtcact
      601 gcccgggttt gggtggtaaa gaacttaacg cctatattac tgcgggtatt gaaaactgcc
      661 acgaaagtta tcagctggaa gaaggacgcc ggaaattaca actcggcatg tcgttgatga
      721 tccgcgaagg gtccgctgcc cgcaatctca acgcgctggc accgttgatc aacgaattta
      781 acagcccgca atgcatgctc tgtaccgatg accgtaaccc gtgggagatc gcccatgaag
      841 gacacatcga tgccttaatt cgccgcctga tcgaacaaca caatgtgccg ctgcatgtgg
      901 catatcgcgt cgccagctgg tcgacggcgc gccactttgg tctgaatcac ctcggcttac
      961 tggcacccgg caagcaggcc gatatcgtcc tgttgagcga tgcgcgtaag gtcacggtgc
     1021 agcaggtact ggtgaaaggc gagccgattg atgcgcaaac cttacaggcg gaagagtcgg
     1081 cgagactggc acaatccgct ccgccatatg gcaacaccat tgcccgccag ccagtttccg
     1141 ccagcgactt tgccctgcaa tttacgcccg gaaaacgcta tcgggtcatt gacgtcatcc
     1201 ataacgaatt gattacgcac tcccactcca gcgtctacag cgaaaatggt tttgatcgcg
     1261 atgatgtgag ctttattgcc gtacttgagc gttacgggca acggctggct ccggcttgtg
     1321 gtttgcttgg cggctttgga ctgaatgaag gtgcgctggc tgcgacggtc agccatgaca
     1381 gccataatat tgtggtgatc ggtcgcagtg ccgaagagat ggcgctggcg gtcaatcagg
     1441 tgattcagga tggcggcggg ctgtgcgtgg tacgtaacgg ccaggtccaa agtcatctgc
     1501 cgttgcccat tgccggcctg atgagcaccg acacggcgca gtcgctggcg gaacaaattg
     1561 acgccttgaa agccgccgcc cgtgaatgcg gtccgttacc cgatgagccg tttattcaga
     1621 tggcgtttct ttctctgcca gtgatccccg cgctaaaact aaccagtcag gggctatttg
     1681 atggcgagaa gtttgccttc actacgctgg aagtcacgga ataataaaaa agcccggcgt
     1741 catgccgggc aaaagtcacc agttacgttt atgccactgt caactgctga atttttttct
     1801 cgcggcggat tttgcgttct tccatcaccg ccactatcgc catcaggcag atacaaccaa
     1861 tcgcggcgat atcaagtgcg gcgaatgtgc ctgcccagcc ggtaaggccg aataccggcg
     1921 tcccatcggc aatcattccc agacctaact tggcaaagct gtcaccaatc aggtaagcaa
     1981 aggtgccttt aataccatcg gcagcgccaa tcgctttttt aggtacaaag ccaacagcag
     2041 ccacaccaat caacaattgc gggccaaaga ccaggaaacc caacgcaaag agagaagcca
     2101 gatagatata ttcgttgctg gcatgttgat acacaccgag cgtggcgata atcagcgcca
     2161 gcgcgatgca ggccaccagg ccacggcgac cgttcgccag gtcagagagc cagccccaca
     2221 gcagcgtacc gaccagcgca ccagcttcaa acagcgtaaa gccctgaatc gccaccgctt
     2281 tagagagttt cagttcctgg aacgcgtata cggttgacca ctggtcgata ccaatacgta
     2341 ccacatagag gaaaatgttg gcgaagcaca gcagccagat cactttgttt ttcagcacat
     2401 actcaacaaa gatctgccac ttggtcatat cggtagattc tgtctctttg tcctcttcgc
     2461 tgatctcctc gccgaacagt tcttcagctt tgccgaggcc ataagattcc ggggagtcgc
     2521 tgccgtaacg caggccgata aaaccgacaa tcagcgcgat aatcgacggg aagataaaca
     2581 tgccgatgac atggccatcg aacaggtaat ttgccccgaa cagcgccaca cctgctgcgc
     2641 ctgcaccgcc aaggttgtga gaaatattcc agaaaccgag gaatgtcccg cgtttacgac
     2701 gcggcgtcca tttggtgatg gtggagtaac tgcacgaacc gccggtactc tggaaaaagc
     2761 cgcttaaggc gtagaaggca atcatcagga acaggctaac cgagccgctg cccatactgg
     2821 cactgaagcc cagcatacaa atggcagaca ggatcagcat gaacggcagg aattgtttgg
     2881 tgtttttgcc gtcggcgtag taggaaacca gcgttttacc cacgccataa gtgatggaga
     2941 aacccaggcc gatcatcccc agctgcgtca tgctcaaccc gtaggtcgag atcatgtcgt
     3001 tctgcgcgat gttaaagttc ttgcgaatca ggtacatcgt caggtagccg ataaagacca
     3061 ccaggtagga ttgcatgaac ggtttgaacc acattttgcg ccgcacttcg agcggaaggt
     3121 ccagggtcgg cttgcgaacc tggtttaaga aagccagcat gggttactcc tgaaatgaat
     3181 acctgcgtga ggcatgcatt gtaaaaatca gcagcaaatc aggcctgaga tagcgtccag
     3241 gcaaaacctg gaaaatttct tagttttgcc ggatatgaag tgaaaaggtg agatgcatca
     3301 cgcttcgcgc ggtgcctggg cgttcaaaaa gggcagtaac agcagtgcgg aaatcccggc
     3361 ggcgatagag ataaccacaa aaaatccgct ccagtgccag gtatcgagta ctttcgccag
     3421 cggccaacca gcaagcgacg cccccagata agcaaacaag ccgacaaacc ccgtcgccgc
     3481 ccctgccgcc tctttgtggg aacactctgc cgccgccatg ccgattaaca tctgcgggcc
     3541 aaagacaaaa aaaccaatgg tgaagaagca ggttgcctgc atcacgtagc tggcaaatgg
     3601 catcagccac agcgagccga ctgaaagcaa aattccggcg gcgaaaatca aattcatcgg
     3661 cccacggttg ccgttaaaca atttgtccga gccccaaccg gctaccagcg caccgataaa
     3721 tccgcccagt tcaaacatcg tcactgccgt attcgccgtg accagatcga cgcccagtgt
     3781 ctcggacata tacaaattgc cccagtcgtt gatcgccgcc cggaccacat agaccagcac
     3841 atagcaaaac gaaagcagcc agatatacgg attcagcaac acatatttgg tgaggatctc
     3901 tttacgcgtc aaccctgccc cttcttgttg ttgagcaatt tccagcgcgt cgtgtcgcca
     3961 ttcaccgacc gccggtaaac ctaacgcctg cgggcgatcg cgtagccgcc agcagagaaa
     4021 aatccccacg actatcgcca tacaaccagc aatcatcatc ccggcacgcc agccgtaatg
     4081 cagcgcagcc gctgccatca caatgggaat gagtgcgccg ccgacgttat gcgccgtgtt
     4141 ccataatgcc caccaaccgc cgcgctcggt acgtgaatac caggccgtta acagacgcgc
     4201 acacaccggt gaaccccagc cctggaaaaa ggcgttcagc acccagagca cggcaaacgc
     4261 ccatagcgac gtcgagaagc caaacagaat gttgatgatg cccgtggcga taagccctat
     4321 ccccataaaa taacgggcat ttgagcgatc gctgacaatg ccggagacaa acttcgacac
     4381 gccgtaggta atgtaaaaca gggtcgctaa caggccgata tcgctacggc tgagcacgcc
     4441 gttagcaagg atttctggta cggcggcgtt aaaactttta cgcgtgaagt aaaacagcgc
     4501 gtaacccagc cagatggtca gcagaatatg ccgacgccag tagcgatagc gggcatcaat
     4561 ttcatgtttg tcagtcatta atggcgcatc ggcaggcgct ttcagaaacg gcaacatcat
     4621 cgaaccttag acatagcgtt gaggtagaga aacgctgaca cgcgtgccgt gcagacagga
     4681 aatgtgtaat gtgccgccca gcgccgttac gcgctcgcgc attccggtga ggccaaaacc
     4741 ttgttgcccg gaacccggcg gcaaaccgct gccatcgtct tcaatcacca gcatcaaccg
     4801 ttcatcctgc tgccagcctt gcagcgtgac cgcgctggca tcagcatgtt tcacaatgtt
     4861 gttcagccct tcctggcaga cacgaaacag cgtcacgcgc tggttttcac ttaatgctga
     4921 ttcatcgatt cgccattcga gatggctgac aatgccgcgc ccttccagct ccatttcccg
     4981 catcagtgag cggatggcct gctccaggga gagatcatcc aactggcgcg gacgtaaccg
     5041 tcctaataaa cggcgcaccg cgtcgtacac gcccagcgac agttgttcga tgagctgccc
     5101 gctctgcttc acgctggcgt tatctgccgc cagccgctga acaatgcccg cctgagtacg
     5161 aatggctgtg atggtctgac cgatatcatc atgcagctca cgcgccacat cacggcgcac
     5221 gctctcttcg gtttccagca accgttcagc cagatgctga ttgcgcgcca gttccttttg
     5281 cagcgactgg ttaagttcac gcaaccgctg gatgccagcg ccaagcaaca accctgtcag
     5341 actttgcacc agcagcgaga gcaataaatc caccggatga tcgcgccagg tttgactggc
     5401 gatcagcgcg atggcgttca tcaacgtcgc aatcagcgcc ccttgccaac catagtgcca
     5461 ggccagcgcg ataatcggca gcgccagaca aaatggcgta aagcgcgaca gttcgtccgg
     5521 caatcccaac tggagccaga gactgataac aaacagcagc aagtaccaga ccagatgtcg
     5581 cccgcgccag ttgatcggct gagaaaccag tgacgggccg agcggcagcc aggtgttatt
     5641 ggcgagatag tgccagaaca ccagacatat cggggccagc gtcaggccgc cagttaaagt
     5701 cagcaacagc gcattccacg actctttgcc gtgccaaagc cagggcagcg actgcaacaa
     5761 cgccgccgcc gttaacgccg caccctgtaa cagcaaggtg cgccagtcac gctgatggcg
     5821 atagcgcgag atcagcgcca cgggcagtaa cgtcagtaaa ctaccgatca tcaataacgg
     5881 aaaatgggtt aaaccgaccg cctgcgttag ccagtaaatc agcagccact ccgcgcccag
     5941 cagcaccggc cagtatccgc gcgggcattg cagcattagc cccagacgca gaccaaacgg
     6001 aaataacagc accgccatat cagggcgctc aaccaggtgc aggctgatac tccacaggca
     6061 aaaccatgcg gcagagaaga taaaaaagca ggcaataacg gtaattaagc gggagaacaa
     6121 cgtcttcatc accagccatc aaacatgcgg cgcgccagct ctacgtcgtt actgacaccc
     6181 agtttttcca tcagattggc gcgatggacg tgtaccgttt tcggtgacaa gcccagttcg
     6241 gcggcaatct ctttcaccgc cattccttgc gccagttttt ccgccacctg gcgttcacgt
     6301 ttggttagcg ggtcctgacg accggatgcc agtttaatgg caatatccgg cgtcagataa
     6361 cagccgcccg tggcaaccgt atgcaccgca gcaatgagtt catccgggct acagcgtttg
     6421 gaaagaaagc cgcgtgcccc cgcgttaagc gcctgctcaa ccagcgcagg actgtcgtga
     6481 acggagagca taatcgtcgc catacctttc ggcagctggc ttagcagctc cagaccggag
     6541 atatcgggca tggagatatc gcaaatacac acctgcacac cgcgccccgg cagccccgcc
     6601 agcgcctcgc gccccgaacc aaactcggca actacctgca aatcaggttc cagccccagc
     6661 agctgcgcaa agccggagcg gacgatgagg tgatcgtcta taagggcaac ggtgatcatg
     6721 gtcttgtcct ggcgggtaaa aaaaaacgcg cttaccttaa cgataagcgc gatgttgttc
     6781 aagccttgag cggttactga aaaaacaccg caatcttgtt aaacatagtc ggatcggact
     6841 gattacgctg cactttcacc acatcttcca gcttatcgat ttggcttatc atctgctcca
     6901 gacgctggtc gtcattgacc agtagccaga tgtggctttt gtcgctgtcc tgaatcggca
     6961 ggcagagaat gccttcaacg ttaaaagcgc gacgggcaaa aaggccacaa acgtgggtca
     7021 ttacgcccgg atggttgcga acggtgagtt ccagaatcac gttgtcatga gttgtgtttt
     7081 gcatggctta ttcccccacc atttcagtat tcgccgcacc aggcggcacc atcgggtaaa
     7141 ctttttcttc ggcatcaatg cgcacatgga tcagcgccgg accagggcga ttgatgattt
     7201 cctgcaatgc agcctgcgga tcggcttcgt tattcaaatc acaggtttcg aggccgaatc
     7261 cggcggcaat ctgcataaag ttgattttgc ccggataggt ggcggcaaaa acgccctgct
     7321 cgtagaacag actctgttgc tgatgtacca gccccagcgc ttcgttgttc atcaggatga
     7381 ttttgacatc cagctgattt tcactggcgg tcgccatctc ctgaatattc atcatcaggc
     7441 tgccgtcgcc ggagaaacac aacactttgc gatccgagtt cgccagcgcc gcgccaatcg
     7501 ccgcaggcag gccaaaaccc atcgtgccca gcccaccgga ggtcagccac tggcgtgggc
     7561 gattgagcgg ataagcttgc gcggtccaca tctgatgctg acccacatcg gtggtgataa
     7621 tcgcattgtc atcgacacag gcggcaacgg cgttgatcag gccgtaatgg cttaatggat
     7681 cgcacgcttt cgggattgga cacgggaatt cgcgctgcaa atccgctacc aactggtgcc
     7741 actctgcacg cggttgcgct tccacctgcg ggatcaattg cgccagcacg tcatcaacat
     7801 ccgcctgaat cgccacatgc ggctgcttga ttttacccag ctctgcgcga tcgatatcga
     7861 catgaataat tttggcattc ggacagaact gctcggtttt gccaatcgcc cggtcatcaa
     7921 aacgcgcacc gagcactatc aacaaatccg cctcctgcaa aatatagttg gtgctgcgca
     7981 cgccgtgcat ccccagcata cccagcgaca acggatgcgc ttttggcaac atgcccagcg
     8041 ccattaaagt catggtggta ggcagttgcg ctttctccgc cagttcacgc acccgtgcgg
     8101 gcgcattgat cacaccgccg cccagataaa gcaccgggcg tttggcagcg ttaatcatcg
     8161 ccgctgcgtc acgaatgctt tcttcgctaa aggcgggggc ggcggctttt tctgccatag
     8221 cgggctgtgt ttcaatctca aaaactgccg tttgcacatc cttaggaatg tctatccaca
     8281 ccgggcctgg gcggcctgat tgcgcaatgc ggaaggcatc gctcatgacc tgcgggagtt
     8341 cttcgatatg tctgaccaga tagttgtgtt tggtgatggg gatagagatg ccgtaggtgt
     8401 ccacttcctg gaaggcgtcg gtgccgatca tcgaggcggg aacctgaccg gtgatgcaaa
     8461 tcagcgggat ggagtccagc cgcgcatcgg caatggcggt caccaggtta gtcgcacccg
     8521 gtccgctaca ggccatacag accgccggtt taccgtcggt gcgcgccatc ccctgagcga
     8581 taaatcccgc gccctgttca tgacgggcca gaatatggcg gatttgcgtg ctttggctta
     8641 aggcatcgta aacaggcagg atagaaccgc ccggaatgcc cgtcacaatc ttaatgccct
     8701 gctgttccag gaaatgaacg ataaattctg cgccggtaaa gcgcttacgc gtcgatgttg
     8761 tgcccgaact tgccatgctc cagtcctttt cttctgggcc gactttccgg gaggtgctta
     8821 aacgaaaaac cccgcccggt ttgcgccggc ggggttttgg aatcgtgtgt tgttccagtc
     8881 cctacggcgc attgccgacg accaccacca cacgcacgac gaccactgcg gcggatagcg
     8941 cagttggtag tagttttgcg ttgaacatgg aagtagtcat tggggacctt gttggttttg
     9001 tgtttaacaa tatttataca agcacagctt tacaggggag acaatggaaa atttttcagc
     9061 aagggaaaat tgaggggctg atcacgtttt gtactgaatt gcaggtaaca aaaaaccccg
     9121 ccggagcgag gtttcatcag ccgcctgcgg ctggtaaccg caaagcacac tgtattatgt
     9181 caacactgaa agtatacgtg ttccgcgcag aacgcgcaat ttcggcacga attttgacgt
     9241 atttagggca tagttgagta tcgatcacag tttgcgtttt gtccaaatat tactgtttat
     9301 ttatacagta aacttctata atatcactgt acgcaatgtg ttatgcgggg gccgcatcgt
     9361 tacccggcgc actaagtcct ggctgaaacg ggtggtgccg tcagcgcctt aaccccgcgt
     9421 gagcacactg tgttatgtca acaagcacaa cgtttctcct taagataccg cgtgcacaac
     9481 agctggcaac aggcagcgga aaggtacgtc agctggcagt gctcctgaac cacaggagac
     9541 gcgtatgagc ctggtggata tcgccattct tatcctcaaa ctcattgttg cagcactgca
     9601 actgcttgat gctgttctga aatacctgaa gtaattcaga ttcaagtcgc accaaagggg
     9661 agcgggtaac cgctcccctt ttatatttag cgtgcgggtt ggagttgggg gcttcttcta
     9721 ccagcagaat gtttgcacct gcgctgcctg tgcccctcac cctagccctc tccccaaagg
     9781 ggcgagggga ccgtcccgtg caatgtttag ctcattttct gaaagggaac ggtggtttcc
     9841 ccctcgcccc tttggggaga gggttacagt tcgcacgtaa tccctcccgg caacatttat
     9901 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
     9961 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    10021 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    10081 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    10141 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    10201 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgatt tccgaccgcg
    10261 tgggccgccg accggtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    10321 cggtcacgac ctccagtttg acggtgttga ttgccgccag cgcgatgcag gggatgggca
    10381 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgaga tttatatgaa cggacacagt
    10441 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    10501 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    10561 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    10621 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    10681 actgttattt gctgatgctg attggcggtc tggccgggat tgccgccttt gaagcctgct
    10741 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    10801 ttctgccgat tccggcagcg ttttttggcg catggtttgc cggacgtccc aataaacgct
    10861 tctccacgtt aatgtggcag tcggttatct gctgcttgct ggctggcttg ctgatgtgga
    10921 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    10981 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    11041 tcctggcggg cacggctggc gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    11101 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    11161 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    11221 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    11281 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    11341 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgg cgagcgtgcg ggcacaggcg
    11401 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    11461 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    11521 ctcgaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    11581 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    11641 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagttt
    11701 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    11761 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    11821 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    11881 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gagaatgcca
    11941 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    12001 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagt
    12061 cctgggtcgg caatgcgtct ggctttgcgg ctatccggca tacaacacca gtcccataac
    12121 gatcaccgcc acgaccatca atattaagct aatgattaat aagctgttgg tgtagggcag
    12181 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacatcgc
    12241 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    12301 gaaatctggc gcaagttggt caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    12361 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    12421 gaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    12481 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc cctacaagga
    12541 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    12601 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    12661 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    12721 ataaaaggcg aaaaaattac taccgtggag aaaaaagagg acgtagatgt cgataaaatc
    12781 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    12841 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggcgg cgggacgctg
    12901 gctttccata ccttgctttt tggtatcggc atcgtgtgca gcattttgct gatattcctt
    12961 atcttttcct tcctgactgg cggttcactg gtttaagcag cctgaataat gcccgccggg
    13021 tgttcatcag cgggcattat cgtcagaact tctgtttttt ctcttccact ttcactcctt
    13081 gcgtcggtag gccggtgtca tagtcacgta ccaccggcga atagccatct tgtgggcgtg
    13141 gacgaaatgc ccccatccag cgcggcagtg catctttacg ccacggacgc aggctccatt
    13201 ggtaactgcg gaacggatcg cgaattttgt ccatgtaatc caataaggcg tcatgcattt
    13261 tgctgcgaac gtctgcaaaa cggatatcat cgatcaggtt atgcatttca tttgggtcat
    13321 tacggcgatc gtaaagttca tcactggtga agaggttgag taccagttta aagtcatccg
    13381 tcacccagca acgcaccgga ataaaaccgc caaagctgtc atgctcaatc tcgtagcggt
    13441 taaattccac cattacaccg cgtggctctt tcacggcaag gatattttcc cccggcagaa
    13501 tctctggttt ttcaatatct gccagcgcca tcattgtcgg cagtaaatcg atatgactga
    13561 ctggcgtatc gacctgtcgc cgctcccctt gcggcgaacg gatgatcagc ggaatacggg
    13621 tgatgtcgtc atacatcgcc gctcctttac tgatcagctt atgtgcgccc atcatttcgc
    13681 cgtgatcgga agtataaatg acccacgtat tttcacgttg ctctggcgtt aaggcattga
    13741 tgacccgtcc gatttggtca tcaacaaagt cattacaggc aaaatagagc ggatggtgat
    13801 aaagcccgtc atcaccgact ggcgatggca tcgcctgcgc ccataagcgg tgatgttccg
    13861 gtttgttcgc caggtcatcc tgagctttct cgcccagatc gtagtaaaaa tcagcgtatt
    13921 tctctaaata ctccaccgga taggtgaacg ggtgatgcgg ctcatcatac gaaaccacca
    13981 tcaggaaggg ttcctcggcg cgcgcgggct gttgcagaaa atctaccgcc cgattgctga
    14041 tgcggtgcgc ccaggtgaag gtttcgtcga tatggttcgc ctgtaaatcc tcaacgctgt
    14101 ttaggccatt gcgccacagg ctgatctctt tttccgtcag ttcgctaagg tagttcgccc
    14161 catcgaacca gtaatcagcg tcccactccg gcggacactc gccagtgccg aaatagtcat
    14221 gaccgtcgag atgccatttg ccgatgtaac aggtgtgata gccggcatcc ttaaagtagc
    14281 gccccatagt ggagatgttt ttgcctggcg cgacgttgtt ggtccacggg ccggactggt
    14341 tagcgtagat accggtaaat agtccggcgc gtgcaggcgt acaaaccggt gaacaggtgt
    14401 aggcggaatt aaagcgaata ccttccgccg ccagactatc aatattttgc gtattcagcg
    14461 gtttaccgct atagcaaccg accatattgg tggcctgggt atcggttatg atgaacagaa
    14521 aattggggcg tttcataggc tatccttcgc gtcgggagta aaggttacgg ctgggtcgtg
    14581 acgatgccgc cagctgtcaa aaatcaggta gacaatcact acggcggcaa tgaaataact
    14641 gatcatcgct aaccagcgcg taccgtagcc gccgaattca gccagcccgg catagacgcc
    14701 aatcatcgcg aacaggatgc caattgacgc gatcttgacg tttctccacg gtttcatgtc
    14761 caccgcaaac gcatctttga aggtgaacgg cgtggcgcgc ggtttgataa aaccgatcac
    14821 cagcatcacg accacgttga tgcagaacgt acaggccagc acatagagga aatggaagtc
    14881 gaacttcacc agatagttga tggtgatgta gctgattatg ccaatcccca tcgccacttt
    14941 tgccgccagc gccgggatgc gcgggaagaa aaagcccata atgatgatgg taaccagcgg
    15001 cacgttgtaa atgccgttga gctgtttcat ccagctatac agcccctgcg gcgcgttggc
    15061 gatccacggc gcgaccagca ccgaaacgat agcgataaag aaaccaaatt tgcgcccgac
    15121 ggtgaccagc tgctgcggct cggcattctg gttaatgatg cgacggtaaa tacccatact
    15181 gaataacgta ctggcgctat tcagaaagcc gttgaaggta ctgatcaccg caccaaataa
    15241 caccgcgccg aagaaaccca ccattggcac tggcagaacg ttattgacca gcgtcgggta
    15301 ggccatgtcg gctttcggta aatcctgata cagatgaaac gcaatcaacc ctggcagcac
    15361 cagtaccagc gggtcgagca ttttcagcac cgccgttaac agcgccccct tttgcccttc
    15421 cgccaggctt tttgacgcca gcgtgcgttg cacgatgccc tgatttgtac accagtaaaa
    15481 ggtgttcacc agaatcaaac cggtaaatgc cgcgccaatc ggcaagggat cggtcgggcc
    15541 accgattgag tttaatttct cggcgtgaac ggtggtgagt tgctcaatgc cctgcataaa
    15601 gctgcccttg cccatcgcga tcaggccaaa taccggcacc atcaacccgc cgataaccag
    15661 cccaataccg ttgatggagt ccgccactgc cattgcgcgc agtccgccga tcaccgcata
    15721 caaaattccc gccagaccaa gcaaaattac cagtagccag atagccgcac cgtgagaaat
    15781 ctgtagcgat tccccgacgt gaaacaggct gttgagcgcc aacgcgccgg agtagagaac
    15841 aatcggcaga aagcagacgc cggtggcaat gaggaagcag aagtcgatga taatacgcgt
    15901 cgttttatca taacgttcct ccagaaaatc ggggatggtg gcaatgccgc gcttcagata
    15961 gcgcggtaaa aagattagcg cgaggaagat cagcgtcacc gctgaagtca cttcccagcc
    16021 catcaccgac atgccgcttt tgtaggcctg cccggaaagg ccgaccagtt gttccgtgga
    16081 aaggttggtt agcattaacg aagcggcaat aaccggcgct tttagtgaac gtccggcaag
    16141 aaaatagcct tgttgcgatc cggtatctat tttgcggacc ttccaccagg tgatcaccgc
    16201 caccagcagc gtaaaaccga caaaactcaa gatttgtaac gaattcatct catagccctt
    16261 atttatgttt atgtcccgga cgcatgaata tagggagagg taaaatgcgt cgggtcaggc
    16321 agtcagattc ttaaaataca aacgtcgtat ccctgaacgg attgttttct gttaagttca
    16381 ggttgtgagc ataattcgcc ggagggatat taaaatgaat ggaaaattgc aaagttcaga
    16441 tgtaaaaaac gaaactccgt acaatattcc cttattgatt aacgaaaatg tgatctccag
    16501 cggaatttct ctgatctcgc tctggcatac ctacgccgac gagcattacc gggtgatctg
    16561 gccgcgggac aagaagaaac cgcttatcgc caactcatgg gttgcggtgt ataccgtaca
    16621 aggatgtggg aaaattcttt taaagaatgg cgaacaaata acactgcatg gcaactgtat
    16681 tatattctta aagccaatgg atattcactc ttatcactgt gaaggtttag tctgggaaca
    16741 gtactggatg gaatttaccc ccaccagtat gatggatatt cccgttggtc agcaaagcgt
    16801 tatttataat ggcgaaattt ataatcagga actcaccgaa gttgctgagt taataacttc
    16861 accagaagca ataaaaaata atctggcagt cgcttttctg acgaaaatta tttatcagtg
    16921 gatttgtctt atgtacgcag atggtaaaaa agatccacaa cggcggcaaa ttgaaaaatt
    16981 aattgccact ttacatgcca gtctgcaaca acgctggagc gtagctgata tggctgccac
    17041 gatcccctgt agcgaagcct ggttgcgtcg tctgttttta cgctataccg gcaagacgcc
    17101 gaaagaatat tacctcgatg cgcgtctgga tctggcgcta tcgctattaa agcaacaagg
    17161 aaactcggtt ggcgaagtcg ctgatacgct caacttcttc gactcctttc atttcagcaa
    17221 agcctttaaa cataaatttg gttatgcgcc gtcagccgtg ctaaagaata cggaccagca
    17281 cccaacggat gccagtccac acaattaacg taactctggc cagtacgctt tgttggcctc
    17341 cagcaactca tccagaatag ctttggcgac cgaggcgctc ggtaccgttt tcgacaacgt
    17401 aatcgcctgc cacaggtgct gatatgaacg ctgttcccag gcgtccacga ccagtttttc
    17461 caccgccact tgctgactca ttaacccttt ctgaaactgc gggatatcgc cgaccaccag
    17521 tggttcaggc cgtagtagcg cacgcgcatc tgtttgcgat cttgcagccc aacaatttgc
    17581 gccatccggc tttcaatacc gattggcatg tcacagatgt tgaggatttt cgcattcggg
    17641 cgcagacgac gcgtggcttc tgcgacaatg gctgccgggt tggagtagtt gagcatccag
    17701 gcatttggtg aatatttttc catataatcc accagttcca ggacgccgcc aatggaacgc
    17761 atgccgtacg ctattccgcc gggtccgcaa gtttcctgac caacaacacc gtggcgcagt
    17821 gggatttttt catccagttc gcgcatcggg tacttgccga cgcggatgtg cgccatgaca
    17881 aaatcaacgt cgctgaacgc cacttcagga tcggtggtgt aactaaacgc aatgtccggc
    17941 gctttctctt taaggatgac tttacaggct tcggcaatca cttcctgccg cgcgccatcg
    18001 ttatcataaa atttcagtgc acgaagcggg aaacgctcct gattcgccag gagcatcaac
    18061 acgatgcctg gcgtaaaggt gcttccaccg cctgcgacaa ccactgagaa tttggtcatt
    18121 atactgcctc cgtaatggca acattttctg ctgattgatg agaattaatt aagctatcga
    18181 gctgttcacg cagctgggat acatgcagac caatgatcac ctgaatggca tcgccactac
    18241 ggaagacgcc gtgcgctccc agctttttaa agacttcgtc atccagcgtt tgtgacatgt
    18301 catgcagtgc aatgcgtaga cgcgtcgcac aattgttaat gctggagata ttgccgaccc
    18361 cgcccagggc ttgcaggata ccggcagcct gatccagctc tttttttggc tctgccgcgg
    18421 tggtttggcc tcgcgaggct ttgtattcgg cttttgagta gagtttcact tccgcatctt
    18481 cacgtcccgg cgtgcacatg ttgaactgca gaatcagtgt gcggaaaacc acgaagtaca
    18541 gcagggtaaa gcacaaccca atggcgattt gggtcagcat catatccgcg tggttgctga
    18601 acatcgggat ccagttttgc ggtaaaacct ggtcaatcag acctccgccc atgttgccca
    18661 ccacaccaaa gagatacatc acggtcgaca ttgaggccgc aagcacagcg tgtaccgcaa
    18721 acagcaacgg tgaaatgaac aggaaggtaa attccagcgg ttcggtaatt cccaccagca
    18781 tggcggttaa ggttgcggga atcagcaagc ccgccacttt tacccgattt tccggtgcgg
    18841 cagtgaagta catcgctaaa gaaatgccca cggcaccaaa gatttttgag ttaccgtgca
    18901 gggcaaaacc gccttccggg aacaacgatt tcagcggctc ggcgctcaga ctgaactctt
    18961 gcagatgctg cgcccagtac atctgaatgc cgccttcaac agctgccgga ccaaagataa
    19021 actgtccgta gatgaagtgg tgtaaaccgg ttgggatcag aatacgttcg aggaaggtgt
    19081 aaacccacac cccaagcgca cccgccgaac gcaggaacgc ttgcagagat tcaatcccca
    19141 tttgtacttt tggccagccg agcaatgtca gccaggcaca ggggatcatc accaggaagg
    19201 cgataatcac cacataagac gtcccctgga aaatgccgag aaaaaccggc agttttttat
    19261 cgaacagacg gttatgcagc gccgtcacaa tgccggaaat gataattgcg ccgataatgc
    19321 tggtatcgag ggttttaatc ccggccatca ttgtcagacc gctacctgcc accgcgtcct
    19381 gagtgaaatc gacgccgaag tagcttcccc aggtcattcc catcgcgttg atgaaatagt
    19441 tccaggtcag gaaactcacc atcaccgcca gacaagcacg cccctgcgct tgcttagcaa
    19501 ggccaatggg taaaccgaca gcaaaaatca gcggcatatt acggaatacc gtccaaccgc
    19561 cctcttcaat aatgtgtacg atttgcgcga ataaactgtt cggatcggtc agtgattccc
    19621 cgacaaacat cgggttttgc agcaagatgg caagacccac cacaatcccg gcgaagggaa
    19681 acagcagcac tggcgtgaac atcgcgccgc caaagcgttg aatttgactg agcatttcca
    19741 catcctcttt tctcaattct gtaagtagga agcctgattg gttatgcggc aggcctggag
    19801 cgaggatagg aagatgcgat cggcagaaca tgacgaccca atgggattcg tgatcgcttt
    19861 catgcttttc gttgggcggt agagatctac gttttcgcga aaaagtagac atctgtgagc
    19921 actggtacac agcatcgaac acatctttaa aaaaaagatg ttttttcaat cgattaagca
    19981 gaacttgtgg gcgcattacc cgggcttgca ggcaaaaaag agatctagag atgatctaca
    20041 aaagcattgc ggagcggtta agaattcgac ttaattccgc ggatttcacg ctaaacagcc
    20101 ttcttcccgg tgaaaaaaag ttggcggaag aatttgcagt atcccggatg accatccgta
    20161 aagcgattga cctgctggta gcgtggggat tggtggtccg ccgccacggc agcggcactt
    20221 acctggtgcg caaagatgta ctgcatcaaa ccgccagcct gaccgggctg gtggaggtgt
    20281 taaaacggca gggaaaaacg gtcaccagcc aggtgctgat ttttgaaatc atgcctgcgc
    20341 ctccggccat tgccagccag ttacggattc aaatcaacga gcagatctac ttctcccgtc
    20401 gcgttcgttt tgtggaaggg aaaccgctga tgctggaaga cagctatatg ccggtaaaac
    20461 tgttccgtaa tctttcgctg caacatctgg aagggtcgaa gtttgaatat attgaacaag
    20521 agtgcgggat tttgattggc ggtaattatg aaagcctgac gccagtgctc gccgatagac
    20581 tgctggcgcg gcaaatgaag gtagcggaac acacgccact gctgcggatc acctcgttgt
    20641 catatagcga gagcggggag tttttgaatt attcagtgat gttcagaaat gccagcgaat
    20701 accaggtgga gtaccattta cggcgactcc acccggaaaa gagttaaccg atactccaga
    20761 agagcaccgc cagtaattgg ggggtgataa tgcgcaggaa catcactaac ggatagacag
    20821 tggcgtaaga gagcgccgcc gcaccgctgg ttggatgaag attattagca aacgccagcg
    20881 ccggaggatc ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa ttcattttgg
    20941 ctaacatccg cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc agggcaccat
    21001 aaccaatcca gcttagccct tcgccattga ccagggtatt tacaaaatcc ccaccagatt
    21061 tcagaccaac gaccgagagg aacagcacga tccccagctc ccgcagcgcg aggttggcac
    21121 ttggcggcat aaaccagtac agcttgccga tactgccgat acgcccgagg atcaacgcca
    21181 taatcagcgg accgcccgcc agccccagtt tcaacgcggc cgggaatcct ggcacaaaga
    21241 cgggaataga acctaacaat acgccaagcc cgatgccaat aaacactggc agcatctgaa
    21301 cctgttgcag tttttgctgc gcattcccca gcacattggc aacggcatca attgcggacg
    21361 gacgccccac caggttgagg atatcgccga actgcaggct gatatcgcca ctggcgacca
    21421 gttcgacccc ggcacggttc aggcgcgaga taacaacgtc atagcgttct ttaaagtgca
    21481 ggtcgcgaat acgttttccg agcacgtttt cattggtgac caccacacgc tcgacgcgca
    21541 aatcagtgcc tttcgtggac agcgaagtat cgacctcctg accaatcacc agttgcgcat
    21601 tatgtaaatc cgctggctga cccaccagat gcagcaaatc gcccagttga ataatggtat
    21661 ctggcgaagg aacttttagg gtttcttcgc gtttcagacg cgagcagata attttgtcgc
    21721 cgttgagaat cggtacatct ttaatggcta aatcatgcag gttagggttc tcaacgcgaa
    21781 tattgatagt cttgatcagc gcgccgccat tggtgcgtga agactcgtgc tgctgagctt
    21841 ctgtctcgac attgacgcgg aaaataaccc gcaacatcca catggtgaac aaaatcccgc
    21901 aaatgccgaa tggatacgcc atcgcgtaac tcatccccat ctgatcgacc atttccattg
    21961 gtgtacccag gtcgcgcaaa atctgctgcc ctgcccccag cgctggcgta ttggtaaccg
    22021 caccggagaa aatccccagc actaccggca gtggaatatc aaacagttta tgcaggatgg
    22081 cggtaaccag accaccgatg atgacgatca gaacagcaaa caggttgagg cgtaatccgg
    22141 agacgcgcaa tgaggcaaag aagcccggcc ctacctgaat cccgatagta taaacgaaca
    22201 ggatcaggcc aaattcctga ataacatgca gcatatcgct acttaatgtc atccccgcct
    22261 gagaaacaaa atggccgacg atgatcccac caaacagcac gccgccaata cctaatccta
    22321 tgccgcgaaa tttgacattg ccgataaaca aaccgacgac tgccaccaaa gccagaatac
    22381 tgaccgttaa tgctatatca ctcatcgtcc atttctcttg agataacatt tttaattgct
    22441 acggattatg gctgaatcgg tgaagcagat ttgccttata gcgcacatta tcacgggaaa
    22501 gaacgtgccg aaatatctta aacaggcgaa atttaacgca ttgttgaaga agaaagacaa
    22561 aaaaagcccc accagaatgg cggggcaaag agaatagcta gttagctatt taacgcggga
    22621 cgttcgctga tagcgatacg ctgcgctgcg atgggttcag gctcattacg aattaaatca
    22681 atatgcagta aaccgtttac gaaggttgcg ccagagactt ccatattttc agccagcgta
    22741 aagctcaggc taaatggctg attcataagc ccttgatgca gccatttttt ctcttctttt
    22801 ggctgctccg gcgtgccttt tacgctcagg cgcgtacctt ccagttgaat ctctaaatct
    22861 tcctgacgga aacctgccag cgcaagggta atgcggtagt ggttatcgtc gcttttctca
    22921 atgttgtacg gcgggaagct ctggctttca ccggcgtttt gcagtgcgtt ggccagtttg
    22981 tcaaaaccga tccattgacg catcagtggg gataaatcga agttacgcat agtcatttct
    23041 ccttctaaga agcgagtaag tacctgcaaa tccgaagatt cgcatatgct ccctgacggc
    23101 gagcatggag atgtcaggcc gcgccaggcg gccttaagga attagttgat ttcgatacgg
    23161 cgcggttttt tcgcttccgg aatcacgcgt tcgagatcga tatacagcaa accatttacc
    23221 aggttagcgc cacgaacatg aatgttctca gctaactgga atttgcgttc aaagttgcgt
    23281 tcagcgatgc cctggtacag ataggtgcgc tctttttgtt cgtcggcgtg agcacctttc
    23341 accaccagca gattatcctg ggcggtaatt tccagttcgc tctcagcaaa accagccaca
    23401 gcgatagcaa tgcggtaatg gttttcgtct accagttcaa cgttatacgg agggtagccg
    23461 ccattactct ggctctggtt gttttctaag tggttaaaca aacggtcaaa tccaatagca
    23521 gaacggtaaa gcggggataa atcaaagtta cgcataatca atagctcctg aaatcagcga
    23581 gaatgtaaga ccttccacaa tggacaggtc aggtagccag aacacccatc aggcgcgtcc
    23641 tcatcggcta cgatgtaaaa atgggtctgg aaatgaactt ttcaagcctt atcagcggac
    23701 ttttttttgc agtttatggt ctattgcata gactgagggg gcagcacgaa acgttaaagt
    23761 gcgatagccg ccacagtgcg acgtaatgat gatgttattt tcagcaaggg atcgctataa
    23821 ctcatcaggc caacacccac gatcaacaga tgaatttatt atgataagaa atgtgttgtt
    23881 agcgttcatg atatgcagcg gaatgacatt actcggagga tgctccagcg tgatgtccca
    23941 taccggaggg aaagaaggaa cgtatcccgg cacccgcgcc agcgcaacca tgattggcga
    24001 cgatgaaacc aactggggca ctaagtcgct ggcgattctc gatatgccgt ttaccgctgt
    24061 gatggacacg cttttactgc cgtgggatgt gttccgcaaa gacagctcgg tgcgctcacg
    24121 cgttgaaaaa agcgaagcca acgcgcaagc caccaatgcg gttattccac cggccagaat
    24181 gcctgataac tgatttagcg atcagtttcg gtgatccaca gctggccacc ttccatccac
    24241 gccagccatt gaccatccgg cgagaagacc acggcgtccg cagaaggtgg attggcgtgg
    24301 ttttcggtta aatactcaac ctcgccgctt tgcgcatggg cacaagcaat tcgattatcc
    24361 agcacaaagc ccaaccattc tcctgacgga tgccagttaa atgcagactg aatatccgtt
    24421 ttgttatggg ttaactggcg cggctcgccg ccctgtggcg agataagcca cagttgcaca
    24481 atgccgttat catcacgcat taaaaacgcg atttgcgtac cctgcggatt acagcgcacc
    24541 cagtggcgcg ggacgttgac taaccccgga taagcccgat ggtgggtaaa ggttaaacgt
    24601 cgctgcacga cgccacgcgg tggtgcgggc agggtggttt ccgttccgct taacggcgca
    24661 tcaccagctg ctttccagcc agcttcatcg tgcggtaact caacgataaa cagctccggc
    24721 actttctcgc cctttggcga aagtgtgtcg ccaataaacg ccagcgcgtg atttcctacc
    24781 catccttctt cataagcacg attgatttca tcactgccag gctgcggcgt gggcgtggtt
    24841 ttactcacca gcacgcacca gtggctaccg ctgtattcac gcggatgctg cttttgtacg
    24901 ttgaccgggc caaacggcgc agcaacaccg acgtttcgca aatccagcgc cggatcgagt
    24961 tcatgcatta catggtcgtt ataggtaaag ctcacccttt caccgttcgg gctaaagaca
    25021 tgcacatggc tgccgccgcg cagcgctcct ggggtgtacg gtgcagtaat atccattgca
    25081 tcgagattgc tcaccttgcc gctttcagca atcacgccgc gacggtgatg gaaatcgtaa
    25141 taccatgttt catcaggatt ttccgggccg tgaataaaga catatttctc tgactttgga
    25201 tgaacggtca ccacgccgac gtgtgcgccc tgtgacgcgc gatagataac ctcgacctcg
    25261 ccggtatgga tattcacacg ctcaatggtc tcgccggtaa acgacgcgcc agaaggacgc
    25321 acgtcaaata ccagccactg gctgtcgggc gtccaggtat tggtattggt gagcaggtga
    25381 ttacggggag caaaggttat ctgtttcatg gcgcggtcct gatacaaaac gggtcccgcc
    25441 atcatacttc acaaggactt cactttcaga caatagtctt ttgccatcgt gatgaatgtg
    25501 agtgaaaaga tggaacattt cgacgtggcg attattggcc tcggcccggc agggtcggcg
    25561 ttggcacgaa agttagccgg caaaatgcag gtgatcgcgt tggataaaaa gcaccagtgt
    25621 ggtactgaag gtttcagcaa accttgtggc ggtctgctgg caccggacgc gcagcgttct
    25681 tttattcgcg atggactgac gctccctgtc gatgtgatcg ccaatccgca gattttcagc
    25741 gtcaaaaccg tcgacgtcgc cgcatcgctc acacgtaact accagcgaag ctatatcaat
    25801 attaatcgcc acgctttcga cttgtggatg aaatcactga tccccgccag cgttgaggtt
    25861 taccacgata gcctgtgccg gaaaatctgg cgtgaggatg ataaatggca tgtcattttt
    25921 cgtgcagacg gttgggagca gcatatttcc gcccgctatc tggtcggtgc cgatggtgcc
    25981 aactcgatgg tgcggcgaca tctctacccg gatcatcaaa tccgtaaata tgtcgctatc
    26041 cagcagtggt ttgcagagaa acatccggta ccgttctact cctgcatctt tgataatgaa
    26101 ataactgact gttattcatg gagtatcagc aaagacggtt attttatctt tggcggtgct
    26161 tatccaatga aagacggtca gacgcgtttc acgacgctga aagagaaaat gagcgccttt
    26221 cagttccagt ttggtaaggc ggtgaaaagc gaaaaatgca cggtgctgtt tccctcgcgc
    26281 tggcaggatt ttgtctgcgg taaggacaac gcctttctga ttggcgaagc ggcaggattt
    26341 atcagcgcca gctcgctgga ggggattagc tatgcgctgg atagcgcaga gattctgcgt
    26401 gcggtgttac tgaagcagcc ggagaagagc aacgccgcct actggcgcgc cacccgcaaa
    26461 ctgcgtttaa aactcttcgg caagatagta aaaagccgat gcctgaccgc accggcttta
    26521 agaaagtgga ttatgcgcag tggtgtggcg catattccac agttgaaaga ttatccaacg
    26581 cgcttcacat cgcccaccag caggatgtaa gagagcgcgc caatcaacgc gacggcggag
    26641 atataaacca gtgcaggtgc gaaaccgtaa ccctgcgcca ggtaccccac caccagcgga
    26701 acggtgatgc cgcccagacc accggcgaag ttaaacacgc cgccggttaa accaatcagg
    26761 cgcatcggtg ccagagaaga gaccagcgac caggtaatcg aagcaaaacc gttgccgaag
    26821 aatgccagcg ccatcaggca cataatcatc atcggatcgt tagtgtagtt agcgcccata
    26881 atgcaggtgg agatcagcaa gccgcagata atcggcgttt tacgcgcaaa gcccagtgaa
    26941 aagcccttac gtaccagcag atccgcgacc cagccagaga gcaggacgcc gacaaacgcc
    27001 gcgaggaatg gcaccgtggt cataaagcct gctttcagcg ccgtgattcc tttttcctgg
    27061 gttaaatagt tcgggaacca ggttaagaaa aaccacagtg tagaagccac cgcaaattgc
    27121 ccaagataaa cgccgatcag tttacgatgg aacaccagtt tccagtcttt ggctgttaac
    27181 ggctgacgcg cctctttctt caccggcgca tcaccatcca ccagaccgcc gccatcacga
    27241 atgtaatcca gttcagcttt gctgatacct ttggtcaggc gcggcggctg ataaacctta
    27301 aaccaaatca gcgaccagat aatgccgata ccaccagtga caatgaacac ccagtgccag
    27361 ctcaacatct cctgaatcca gatcagcagc ggagtcaaaa acgccagacc gacaaactga
    27421 ccagacgtat aaaaaccaac ggcggaagcg cgttcatgtt ccgggaacca gctggtcacc
    27481 atccggttat tggtcgggaa agcgggcgct tcgaaaatac cggttatcgc acgcagacca
    27541 attaatgaca ttaatcccgt ggcaaagccc tggaataaag tcgccactga ccaaccaaat
    27601 atcgcaataa aataagtcac gcgagaacct acgcgatcta aaaaccaacc gccggggatc
    27661 tggcatagcg tataaagcca ggcgaaggcc gaaaatacat agcccatttc cgctttcgta
    27721 atgccgaact cttcctgaat atgggcggaa gccacggcca ggttagcgcg gtcaacataa
    27781 caaatgacca ccgtaataaa gatcatcacc agcgtcagat aacgccgacg ccccggcttt
    27841 gctgcattaa cgggaatatc catagcgagc tttctccaga ttttgggcat agcgaagccg
    27901 ctcaccatgc cctgtaattt acagagggtt atagttatat ttttgtattg ctgttttagt
    27961 gcccgatgag gggcttacgt ggcaggaatt accactctgc tacgctgtta tcttcatgac
    28021 gccagagcgg attacgccag tccggggcat ttttactgaa ctcaataact ttagcttcgt
    28081 cgatttccac acctaagccc ggtttcgtta acggtttaaa gaagccgcca accatactga
    28141 agtcttcttt gtttttcaca aagtcgagta actccgcgcc tttgttgtaa tgaatgccca
    28201 tactttgttc ctgaagtacc gcgttatagg aaacaaagtc gatatgcagg caagccgcca
    28261 gtgcaatcgg tccgagcgga cagtgcggcg caagggtcac atcataggct tctgccattc
    28321 cagcaatttt gtagcattcg gtaataccgc ctgcatggga gagatccggt tgcagaatcg
    28381 aaataccgcc tgcctccagc acgcgtttaa aatcgaaacg tgagaacatg cgctcacccg
    28441 ccgccagtgg aatatgcgtt tgtgccgcca gtttcgggta gtattcggct tgttccgcca
    28501 gcaccggctc ttcaataaac agcgggcgat acggctccag ttctttaatc agcactttcg
    28561 ccattggcgc gctgacgcga ccgtggaaat caagaccaaa ctcaatctga ttgccaaaag
    28621 cttcacgaat ttgtgccacg gtgttgactg ccgcatctac cgcgcgggag ttatcaatta
    28681 gccccagttc ttcacaaccg ttcagtttga aggtatcgaa gccgatttcg cgcagggttt
    28741 taatgccgtc gataacatcc gccggacgat cgccgccgac ccaactgtag gctttaattt
    28801 tgtcgcgaac caggccgccc atcagttgcc agaccggcgc attcagcact ttgcctttga
    28861 tatcccataa cgcctggtcg atcccggcaa tggcgctcat caggattgga ccgccacgat
    28921 aaaatccggc gcgatacatc acttgccata agtcattgat gcgcgaagga tcctgaccaa
    28981 tcaaatagtc acccagctcg tgaactgccg cttccaccgt acgggcgcgg ccttcaatca
    29041 cgggctcgcc ccaaccgacc acgccttcat cggtttcaat tttcaggaac atccagcggg
    29101 gaggtaaacg atacgtggta attttggtga ttttcattgc actgcctctc gatacgcctt
    29161 aacaaatgct gctgcctgct gcgcggtgcg ttctacggac tgcccggcgc gatagagatc
    29221 gctgcctaag cccgccccag cacaacctgc gtctatccac tgcgccaggt tttctggcgt
    29281 cacgccgcca acggcaaaga ctgcgatgtc cgatggcaat accgctttta acgctttgat
    29341 gtattgcgga ccaaaagccg atgacggaaa tattttcagc gcctgcgcgc ccgcttcgag
    29401 cgcggtaaag gcttcggtcg ccgtcgcgca gccggggcag acggtcatgc cgtagcccac
    29461 cgcacggcgg atcacttcac tatggatatt gggcgtaacg atgagctgac agcccatcct
    29521 ggcgagcgca tcgacctgtt caggtttcag taccgtacct gcgccaatca acgccttgtc
    29581 gccatacgca tcaacgatgg cgggaatgct ttgctcccat tgtggggaat tcagcgggat
    29641 ttcaaccgcg tcgaacccgg cgtcaatcac cgcgccaaca tgcgccagcg cctcgtcggg
    29701 cttaatgccg cgcaaaatgg cgatcagcgg aagtttagtt tgccactgca tgagcgatgc
    29761 tccttatacc agcctgaaat gccgtgtcgc ccgccaccgc cgtcacgtcg caacccatcg
    29821 cctgaaaggc ttgctggtag cgcgcggtca gcgatgttcc ggcgacaagg gtgatggcgt
    29881 gttgatgggt cacatagtcg cgcatactgg caacctctgc gccaatcaac aaaccagaga
    29941 gaaattcgct gacctgttcg cggggaagtg ttcccagcac atgcgaggcg cgaacttcaa
    30001 aaagctgcgg caatatggcg ggcgcattaa ggccgcgctc aaggccagcc gcgaaggcat
    30061 cggcagagtt ttcctgcggc ggcaaacctg cgccaatcaa tgagtgattt aacagtaaat
    30121 gatgtagttc accggtcatc acggtgcgaa aatcgttgat ttgctggcta tcggcctgca
    30181 cccatttgca atgcgtaccg ggcataacat aaagagagga aggagccaga gttcgcgcgc
    30241 cgatcaattg tgtttcttcg ccgcgcatca cattgtggtt atcgtcatga gagacgcata
    30301 atccgggaat aatccagata ttgtcgccaa cagacgttaa ttgttcgcca atagacgaaa
    30361 aacgggcagg aacagataaa tacggagcaa ctttccagcc gacattgctg ccgaccattc
    30421 ctgccattac cactggcgta ttctcttcac gccagtcggt cgtgacttct gctaacaccg
    30481 cagccggaga ttttccgttc aggcgcgtga cgcctgcttc tgattgcctg ctctccaggc
    30541 agtggtcgcc ctgataaagc caggcgcgaa gattggtcga tccccagtca attgcgatgt
    30601 agcgagctgt catgtgattt cctttaacct tcgtgtcgag ctggcgatca tggtaagcgc
    30661 cgcctgctct gccgcatcgc cgtcctgatg ccgtatcgca tcgaacagcg ccttatgttc
    30721 ctggagcgtt tgcggcatgt tggcctcatc gcccatccag gttcgttcaa aaaccgcccg
    30781 ctgcagcgaa ctgatcgcaa tgctaagttg ctgtaacacc gggttatgca ccgactgcag
    30841 caccgcctcg tggtagcgaa tatccgcttc gttaaacgct tcgcggtcct gattgttggc
    30901 aatcatctcg ttcagcgccg attcaatctg cgccagatcg ctggaggtcg cgcgctctgc
    30961 tgcccaacgg gcaatcgccg gttccaccag atttcgcact tcgctcatgg cactgataag
    31021 ccgtgggtcg tagtcgtttt ccaacaccca ttgcagtacg tcagtgtcga ggtaattcca
    31081 ctggttacgc ggtgccacaa acgccccgcg ataacgtttc atttcaatca gccgcttcgc
    31141 catcagcgaa cggaacacct cacggatgat gttgcgcgag gttgcaaact cttcacagag
    31201 ctccgcctca gccggaagtg gcgagccagg tacgtatttg ccgtgaacga tctgtttacc
    31261 cagcgtaatg acaatgcgat cggttttatt gagagtcatg gagagtcctt gtgctctgta
    31321 tgttcttctc tactttaccc cgatcgatgc ataacgcggc aactttgtag taccagcgtg
    31381 atgacgttcg cgtttgccgt gtgcgtaatg tagtacaaac ttatactgtt gtactacaat
    31441 ttagatcaca aaaagaacaa tgcataaaaa aatgacatgc gtcgggcaga aatctgaaaa
    31501 gggatatcag gcgctaaaca ggagggaaag aagagtatgc tttcaacggc ttagctactc
    31561 gtttaaagga ttaatcatga agttgaattt taagggattt tttaaggctg ccggtttatt
    31621 cccactggcg ctgatgcttt caggctgtat ctcgtatgct ctggtttccc ataccgcaaa
    31681 gggtagttca ggaaagtatc aatcgcagtc agacaccatc actgggctat cgcaggcaaa
    31741 agatagtaat ggaacaaaag gctatgtttt tgtaggggaa tcgctggatt accttatcac
    31801 tgatggtgcc gatgacatcg ttaagatgct caatgatcca gcacttaacc ggcacaatat
    31861 tcaggttgcc gatgacgcaa gatttgtttt aaatgcgggg aaaaagaaat ttaccggcac
    31921 aatatcgctt tactactact ggaataacga agaagaaaag gcactggcaa cgcattatgg
    31981 ttttgcctgt ggtgttcaac actgtaccag gtcactggaa aacctaaaag gcacaatcca
    32041 tgagaaaaat aaaaacatgg attactcaaa ggtgatggcg ttctaccatc catttaaagt
    32101 gcgattttat gaatactatt cacccagagg cattccggat ggtgtttccg cagcattact
    32161 gccagtgact gttacgctgg acatcattac tgcaccgctg caatttctgg ttgtatatgc
    32221 agtaaaccaa taatcagtaa gcgggcaaac gcgtttatgc tgtttgcccg tccactgatt
    32281 aattcagcac atacttctca atagcaaacg ccacgccatc ttcaaggttg gatttggtga
    32341 caaagttcgc cacttctttc accgaaggaa tagcgttatc catcgccaca ccgacgcctg
    32401 catattcaat cattgcgatg tcgttttcct gatcgccaat cgccatgatt tcttccggtt
    32461 taatacctaa cacgtcggcc agtgatttca cacccgtacc cttgttaacg cgtttatcga
    32521 ggatttcgag gaagtacggc gcacttttca gcacggtata tttctctttc acttcctgcg
    32581 gaatacgcgc aatagcctgg tcgaggatgg cgggttcatc aatcatcatc actttcagga
    32641 actgggtatt ggggtccatt ttctccgctt cgcagaacac cagcggaatg gtggcaacga
    32701 aggattcatg caccgtgtag tagctgatat cgcgatttgc tgtatacagc gtggtgcggt
    32761 ccagggcgtg gaaatgagaa ccgacttcgc gagagagttt ttccaggaaa cggtagtcgt
    32821 catagctgag agcggtttgc gccacggtgc taccatcggc ggccttctgt accaacgcgc
    32881 cgttataagt aatgcagtag tcgcccggct gttccatatg cagctctttc aggtagttgt
    32941 gaacgcctgc ataagggcga cccgtcgtta gcacgacatt cacgccacgg gcgcgagctg
    33001 cggcaatcgc atttttaacg gcgggtgaaa tggtgtgatc gggcagcaga agggtgccat
    33061 ccatatcgat agcaatgagt ttgatagcca tgagttcccc aggtagattg gttcctgacc
    33121 catgctaacg cgattccgct caaaaatcag tacaacaccc gagggaaaag ggggatgcaa
    33181 cgcgggttgc atgctcccca tttgcttagc ggaagagttt ccctttcagc agttccatac
    33241 ctgcggaaag cagatcgtta ttggcttgtg gtgacacttc accttgcggt gagagcgcat
    33301 caataatctt cggcaattgc tctgccagta aactggaagc tgtactggta tccacgccaa
    33361 gtttttgccc gagatcggac accgcatttg tgccgagcgc cgattccagt tgctcgccac
    33421 taaccgattg attgccctgt tgattactca gccaggttga gagaatggcc cctaagccgc
    33481 cactttgcag tttttccagc agcacctgaa tgccgccctg ctcctcaacc caacttaaaa
    33541 tagcctgata tttccccgca tcgcctttca gaaaggcacc gacaacttca tcaaaaagcc
    33601 ccatgataat cacctgtaaa gcgttacgtg ttgacccaaa aagtatagat ttgcgggatg
    33661 ataattgcgg attgcagaaa taaaaagggc ggagatgatc tccgcccttt tcttatagct
    33721 tcttgccgga tgcggcgtga acgccttatc cggcctacaa aatcatgcaa aatcaatata
    33781 ttgcaggagt ctcgtaggac taataagcgt agcgcatcag gcacgctcgc atggttaacg
    33841 ccattaaata tcaatattcg ccgctttcag ggcgttctct tcaataaacg cacgacgcgg
    33901 ttctacggcg tcgcccatca gcgtggtgaa caactggtca gcggcaatcg catccttaac
    33961 ggtaacgcgc agcatacgac gactttccgg gtccatggtg gtttcccaca gctgttccgg
    34021 gttcatctcg cccagacctt tataacgctg gatggagaga ccgcgacgag actctttcac
    34081 cagccagtcc agcgcctgct cgaagctggc taccggctga cgacgctcgc cacgttcgat
    34141 aaatgcatct tcttccagca agccacgcag tttctcaccc agcgtgcaga tacgacgata
    34201 ttcgccacca gtgataaatt cgtgatccag cggatagtca gtatccacac cgtgggtacg
    34261 cacgcgaaca atcggctcga acaggttttg ttcggcattg gtgtggacat caaacttcca
    34321 ctggctgccg tgctgttctt tgtcgttcag ttcgctgacc agcgcgttca cccagcgggt
    34381 aacggtctgc tcatcagaga ggtcggcttc cgtcagcgtc ggctgataga taagctcttt
    34441 cagcattgct ttcggataac gacgctccat gcggttgatc attttctgcg tcgcgttgta
    34501 ctcagacacc aatttctcta acgcttcgcc agccaatgcc ggtgcgctgg cgttggtgtg
    34561 cagcgttgcg ccatccagcg cgatagagat ctggtactga tccatcgctt cgtcgtcttt
    34621 aatgtactgt tcctgcttgc ctttcttcac tttgtacagc ggcggctgag cgatgtagac
    34681 gtgaccgcgt tcaacgattt ccggcatctg acgatagaag aaggtcaaca gcagcgtacg
    34741 aatgtgcgag ccgtcgacgt ccgcatcggt catgatgatg atgctgtgat aacgcagttt
    34801 gtccgggttg tactcgtcac gaccgatacc acagccaagc gcggtgataa gcgtcgccac
    34861 ttcctgagaa gagagcatct tatcgaagcg cgctttctca acgttgagga ttttaccctt
    34921 cagcggcaga atcgcctggt tcttgcggtt acgcccctgc ttcgcagagc cgcccgcgga
    34981 gtccccttcc accaggtaca gttcggaaag cgccggatcg cgttcctggc agtctgccag
    35041 tttgcccggc aggcccgcta agtcgagcgc acctttacgg cgggtcattt cacgcgcgcg
    35101 acgcgccgct tcacgggcac gggcagcatc gataattttg ccaaccacga ttttcgcgtc
    35161 ggttgggttt tccagcaggt attctgccag cagttcgttc atctgctgtt caaccgctga
    35221 tttcacctca gaagaaacca gtttgtcttt ggtctgggag gagaatttcg ggtccggcac
    35281 tttcacggaa acgaccgcaa tcaggccttc acgcgcatcg tcaccggtgg cgctaacttt
    35341 ggcttttttg ctgtagcctt ctttgtccat gtaggcgttc agggtacggg tcattgccgc
    35401 acggaagcct gccaggtgag taccaccgtc acgctgcgga atgttgttgg taaagcagta
    35461 gatgttttcc tggaagccat cgttccactg caacgccact tcgacgccaa taccgtcttt
    35521 ttcggtggag aagtagaaga tattcgggtg gatcggcgtt ttgttcttgt tcagatattc
    35581 aacgaacgcc ttgatgccgc cttcatagtg gaagtggtct tctttgccgt cacgcttgtc
    35641 gcgcagacga atggaaacgc cggagttgag gaacgacaac tcacgcagac gtttcgccag
    35701 aatttcatat tcgaactcgg tcacattggt gaaggtttcg aggctaggcc agaaacgcac
    35761 catggtgccg gttttttcag tctcgccggt aaccgccagc ggggcctgcg gtacaccgtg
    35821 ttcgtagatc tgacggtgaa ttttaccctc gcgctggata accagctcca gtttttgcga
    35881 cagggcgttt actaccgaaa caccaacgcc gtgcagaccg ccggacactt tataggagtt
    35941 atcgtcaaat ttaccgcctg catgcagaac ggtcatgatc acttccgccg ccgatacgcc
    36001 ctcttccggg tgaataccgg tcggaatgcc gcgcccgtca tcctgtacag agacagagtt
    36061 gtcggcgtga atggtgacga taatttcttt acagtgaccc gcgagcgctt cgtcgatagc
    36121 gttatctacc acctcgaata ccatgtggtg cagaccggtg ccgtcatccg tgtcgccgat
    36181 atacataccc gggcgcttac gcaccgcatc cagccctttc aggactttga tactggagga
    36241 gtcataagaa ttcgacatca acgtttctcg ctcatttata cttgggttaa tccgttattt
    36301 tacccttttc cacggtaaac atcttcgaat tttcgtccga catgtctata acgtgttcag
    36361 cactgatcgc gctgacaaag acctgtgatt gcgtcgcttt taagcggctg gcaagcagcc
    36421 cgcgacgctc atcatcaagc tcagaggcaa aatcatctat caggtagaga caccgccgcc
    36481 cgctttcacg ggtgaggaac tctccttgcg ccagacgtaa ggcgcacatc aacagcttaa
    36541 gctgcccacg cgataaggta tcttccaccg gcgcaccgtc ggcgcgaatg cgtaagtccg
    36601 ctttatgcgg gccatgcgcg gtgtaggtta gctggcgatc gcgttcaaaa ttacgttcca
    36661 gcacctcagc atattctgtc tctttctccc agccgcgctg gaaagagaaa gtcagagaaa
    36721 actcagggag aaattgctta caagtatcgg ccatatcagc cgcgataccg gcgctatact
    36781 ccgcgcgcca ggtgctgatt tgctccgcca gcgggatcag ttctttatcc cacgggcgta
    36841 gctgttcgta acgtgtcacc tggcgcagcg ccgcattgcg ctgcttgagc aatcgcttga
    36901 gattgctcca ggcggtgaaa aatccgggtt cgttgtgaaa gcatccccag tcgaggaatg
    36961 ctcttctgta tttggggccg ccgttgagta aagtaaaccc ttctggcgtt atcaactgca
    37021 ttggcatcag gtgcgccagt tccgcgacct tatgcccgtc tgtaccgtcg atgcggactt
    37081 tgctgtcgcc ctgtttgtct ttggttaagc caatcgctgt ctcgcgctct tcgccctgta
    37141 atcgcccgtg gagaacaaac gcctcctgct catggcgaat gacgcgacca atctgcaaac
    37201 tgcgaaacgc ccgaccatgg ccgagcgtat agatggcttc cagcacgctg gttttgccac
    37261 tgccgttggc acctaccaga aagttaaagc cgggagataa agcgagatcc gcggtttcaa
    37321 tgttgcggaa atcgcggatc aacaagcggg tgagggacat tacagtctca ttggcatgac
    37381 aacataggca gccgactgtg atgcggcatc ttcaatctgc acgctggaaa ccgaatcggt
    37441 cagcatcatg cggacgtttt cgcatttcag cgcgttcaga acatccagca catagctgac
    37501 gttgaagccg atttccatct ccgcaccgct ataggtaacg tcgaggatct cttccgcttc
    37561 ttcctgttcc gggttgttgg cggtgatttt cagctggttt tcgctgacat acaggcgcac
    37621 gccgcggaat ttctcgttag agagaattgc cgcacgggca aacgcctgct tgagcagatc
    37681 gcagccagct tccagatgtt tgtccggatt cttcggcaga acgcggcggt aatccgggaa
    37741 gcgaccatcc accagtttgg aggtgaagat aaagtcgcca acgtgggcgc gaatattgtt
    37801 gctgccaatc tgcacgcgca gcggattgtc gccgccgtcg agcatacgca tcagttcaat
    37861 cacgccttta cgcggtacga tcaccgaatg gcttggcaaa gattgaccaa ttggcattga
    37921 acagaccgcc agacggtggc cgtcggttgc cacggtgcgc agttcttcac cttcggtttc
    37981 aaacagcata ccatttaagt aatagcgaac gtcctgatgc gccatcgaaa actgggtcgc
    38041 ttcaatcaga cgcttcatcg ttgcctgcgg cagggtaaat tcgacttcac tctgccagtc
    38101 atcgaggttc gggaaatccg ccgctggcag ggtagacagc gaaaaacggc tacgcccgga
    38161 gcgcaccagc atccgctcac cttccagctg cacggcaatt tccgcgcctt caggcagacc
    38221 acggcagata tcaaagaatt tgcgcgccgg aacggtcgtc gctcctggct cgtgtggctg
    38281 aaccagcgca acgcgtgcca ccatttccat ctcgagatca gtaccggtca gcgacaacgt
    38341 accgtcagca acctgtaaca gcagattacc gagaatcggt agcgtaggac gaccacctaa
    38401 cggaccgctc acctgttgta gcggttttaa taaatgctca cgttctacgg taaatttcat
    38461 aggtttacga tgacaatgtt ctgattaaat ttgaaaaatc ttctttgata tcgtggctct
    38521 cttcacgcaa ctgctcgatc ttacggcagg catgaagcac cgtcgtgtgg tcacgaccac
    38581 caaacgcatc gccaatctcc ggcagactgt ggttagtcag ctctttcgcc agcgccatcg
    38641 ccatctggcg cggacgcgcc accgagcggg atcgacgctt ggaaaggaga tccgcaactt
    38701 tgatcttgta gtactccgcc accgtcttct gaatattgtc gatggtgacc agtttttcct
    38761 gcaatgccag caagtcgcgc agcgcctcac gcacgaagtc gatggtgatc gcccttccgg
    38821 taaagttggc gttggcaatg acgcggttca gcgccccttc cagctcacgt acgttagatc
    38881 gtagacgctt ggcgataaag aacgccactt cacccggcaa acgaatgtcg ttttcgtcgg
    38941 cctttttcat caggatcgcc acacgggttt ccagctctgg cggttcgatc gccacagtca
    39001 gtccccaacc gaagcgggat ttcaaacgat cctcaacgcc gttgatctct ttcggatagc
    39061 gatccgaggt gagaatgatc tgttgattac cttccagcag ggcgttgaag gtatggaaaa
    39121 actcttcctg agatcgttct ttattagcaa aaaactgaat atcgtcgatc agcagtgcat
    39181 ctacggaacg gtagtagcgt ttaaactctt cgatcgcgtt gttttgcagg gctttaacca
    39241 tgtcctgaac aaagcgctcg gagtgcatat aaaccacttt ggcattcggc ttgcgcgcca
    39301 taatgccgtt acccaccgca tgcagcaggt gagttttacc cagacccgtg ccgccataaa
    39361 ggaacaacgg gttataggca ccgccagggt tatccgccac ctggcgagcc gccgcgcgcg
    39421 ccagttggtt agatttacct tcaacgaagt tatcaaacgt gtgtttgacg tttacgttag
    39481 aacgataggt cggttctgcc ggggccggga cgttatccca acctgagcgc gtagaaggcg
    39541 cagcacgttg cggctgcgtt tgcgccacct gtgcaggggc cgcgacgttg ctcgtcactg
    39601 ccgcttgtgg cgtttgcgtc accggtttgg tgccgacttc aaaacgcagc tgtggggcat
    39661 ccgctccgca gaaactggtt agcagtccat tgatattatt aaggtacttg tcccgtaccc
    39721 aatcgaggac aaaacggttt ggcgcgtaca gggccagcgt gttatcgctc agttccgcct
    39781 gcaatgggcg tatccacata ctgaattctg tggctggtaa ctcatcctgc aatcgggcaa
    39841 gacactgctg ccaaagcgaa agtgacacgg cggactccac tcgaacaaaa gtcgataatg
    39901 actaaggctg aaacattcat gattgttgac gtacgtcgaa aagaccctgc ttgagggtga
    39961 cgcacgaacc gctgtctgcg gttatatgcc cgatcaagat cctgcaaaac gatcgggacc
    40021 gcggatcata gcctaaactg cgcaagagat cttctgtttc tcacagattt tcccgattta
    40081 tccacaggac tttccagaac tcgctaagtg taaacgatcc tgccgcgagg cgggcacgat
    40141 ttacgccgca tattggaaaa tttaatgacc atagacaaaa attggcttaa tcgatctaat
    40201 aaagatccag gacgatcctt gcgctttacc catcagcccg tataatcctc cacccggcgc
    40261 gccatgctgg tttccactgg tgtgaggtcg tacattttcc ctgcgaaaag gtgcggaaaa
    40321 gcgcggtaaa taaggaaaga gaattgactc cggagtgtac aattattaca atccggcctc
    40381 tttaatcacc catggcttcg gtgtccatcg tttcattttt cggcggatat ccaataaagc
    40441 cattgaattt attcaagttt aggtagaaat cgccatgaaa cgcacttttc aaccgtctgt
    40501 actgaagcgc aaccgttctc acggcttccg tgctcgtatg gctactaaaa atggtcgtca
    40561 ggttctggca cgtcgtcgtg ctaaaggccg cgctcgtctg accgtttcta agtaataaag
    40621 ctaacccctg agtggttaag ctcgcatttc ccagggagtt acgcttgtta actcccagtc
    40681 aattcacatt cgtcttccag cagccacaac gggctggcac gccgcaaatt accattctcg
    40741 gccgcctgaa ttcgctgggg catccccgta tcggtcttac agtcgccaag aaaaacgttc
    40801 gacgcgccca tgaacgcaat cggattaaac gtctgacgcg tgaaagcttc cgtctgcgcc
    40861 aacatgaact cccggctatg gatttcgtgg tggtggcgaa aaaaggggtt gccgacctcg
    40921 ataaccgtgc tctctcggaa gcgttggaaa aattatggcg ccgccactgt cgcctggctc
    40981 gcgggtcctg atagccctca ttcgggtcta tcaacgcctg attagtccgc tactcgggcc
    41041 gcattgtcgt ttcactccaa cctgttcaag ctacggaatt gaggcattgc gcaggtttgg
    41101 agtgataaaa ggcagttggt tgacggtgaa acgcgtatta aaatgccacc ctttacaccc
    41161 tggtggtgac gatcccgtcc cgcccggacc atttgatacc agagaacact aacgatggat
    41221 tcgcaacgca atcttttagt catcgctttg ctgttcgtgt ctttcatgat ctggcaagcc
    41281 tgggagcagg ataaaaaccc gcaacctcag gcccaacaga ccacgcagac aacgaccacc
    41341 gcagcgggta gcgccgccga ccagggcgta ccggccagtg gccaggggaa actgatctcg
    41401 gttaagaccg acgtgcttga tctgaccatc aacacccgtg gtggtgatgt tgagcaagct
    41461 ctgctgcctg cttacccgaa agagctgaac tctacccagc cgttccagct gctggaaact
    41521 tcaccgcagt ttatttatca ggcacagagc ggtctgaccg gtcgtgatgg cccggataac
    41581 ccggctaacg gcccgcgtcc gctgtataac gttgaaaaag acgcttatgt gctggctgaa
    41641 ggtcaaaacg aactgcaggt gccgatgacg tataccgacg cggcaggcaa cacgtttacc
    41701 aaaacgtttg tcctgaaacg tggtgattac gctgtcaacg tcaactacaa cgtgcagaac
    41761 gctggcgaga aaccgctgga aatctcctcg tttggtcagt tgaagcaatc catcactctg
    41821 ccaccgcatc tcgataccgg aagcagcaac ttcgcactgc acaccttccg tggcgcggcg
    41881 tactccacgc ctgacgagaa gtatgagaaa tacaagttcg ataccattgc cgataacgaa
    41941 aacctgaaca tctcttcgaa aggtggttgg gtggcgatgc tgcaacagta tttcgcgacg
    42001 gcgtggatcc cgcataacga cggtaccaac aacttctata ccgctaatct gggtaacggc
    42061 atcgccgcta tcggctataa atctcagccg gtactggttc agcctggtca gactggcgcg
    42121 atgaacagca ccctgtgggt tggcccggaa atccaggaca aaatggcagc tgttgctccg
    42181 cacctggatc tgaccgttga ttacggttgg ttgtggttca tctctcagcc gctgttcaaa
    42241 ctgctgaaat ggatccatag ctttgtgggt aactggggct tctccattat catcatcacc
    42301 tttatcgttc gtggcatcat gtacccgctg accaaagcgc agtacacctc catggcgaag
    42361 atgcgtatgc tgcagccgaa gattcaggca atgcgtgagc gtctgggtga tgacaaacag
    42421 cgtatcagcc aggaaatgat ggcgctgtac aaagctgaga aggttaaccc gctgggcggc
    42481 tgcttcccgc tgctgatcca gatgccaatc ttcctggcgt tgtactacat gctgatgggt
    42541 tccgttgaac tgcgtcaggc accgtttgca ctgtggatcc acgacctgtc ggcacaggac
    42601 ccgtactaca tcctgccgat cctgatgggc gtaacgatgt tcttcattca gaagatgtcg
    42661 ccgaccacag tgaccgaccc gatgcagcag aagatcatga cctttatgcc ggtcatcttc
    42721 accgtgttct tcctgtggtt cccgtcaggt ctggtgctgt actatatcgt cagcaacctg
    42781 gtaaccatta ttcagcagca gctgatttac cgtggtctgg aaaaacgtgg cctgcatagc
    42841 cgcgagaaga aaaaatcctg attcggtgag ttttcgctaa aataagggcg gtcagttgac
    42901 cgcctttttt cttttcgtag ggcggataag caccgcgcat ccgccacaca aagcaacagg
    42961 aacatcatga gcgataatga cactatcgta gcccaggcca cgcctccggg acgtggcggc
    43021 gttggcatcc tgcgcatctc cggcttcaaa gcccgtgaag ttgccgaaac cgtgctgggt
    43081 aaactaccta agccgcgcta cgccgattat cttccgttta aagacgccga cggcagcgtg
    43141 ctcgatcagg ggattgcgct atggttccct ggcccgaact cgttcaccgg cgaagatgtg
    43201 ctggaactgc aaggtcatgg cggtccggtg atcctcgacc tgctgttaaa acgcattctg
    43261 accattcccg gcctgcggat tgctcgccct ggtgagtttt ccgaacgcgc gtttcttaac
    43321 gataaactcg acttagccca ggccgaggcg attgccgatc ttatcgacgc cagttcggaa
    43381 caggcggccc gttcggcact taactcgctg caaggcgcat tctccgcacg ggtcaaccat
    43441 ctggtggaag ccctcaccca cttgcgcatt tacgtcgaag cggcaattga tttccctgat
    43501 gaagagatcg atttcctctc cgacggcaaa attgaagccc agcttaatga cgtgattgcc
    43561 gatctcgatg cagtgcgtgc tgaagcacgt cagggtagtt tgttgcgcga agggatgaag
    43621 gtggtgattg ccggacgtcc taacgccggt aaatcgagcc tgttaaacgc gctggcgggc
    43681 cgtgaagcag caatcgtaac tgatatcgcc ggaaccacac gtgacgtgct gcgtgagcat
    43741 atccacattg acggaatgcc gctgcatatc atcgataccg ctgggctacg tgaagccagt
    43801 gacgaagtgg aacgtattgg tatcgagcgc gcgtggcagg aaattgaaca ggccgaccgc
    43861 gtgctgttta tggtcgatgg caccacaaca gacgccgtgg atccggcaga gatctggccg
    43921 gaatttatcg cccgtctgcc agcgaaactg ccgatcaccg tggtgcgcaa taaagccgat
    43981 atcaccggcg aaacgctggg aatgagtgaa gtgaacggtc acgcgttaat tcgtctctcg
    44041 gcaaggacag gtgaaggcgt ggaggtgctg cgtaaccatc tcaaacagag catgggcttt
    44101 gacaccaaca tggaaggcgg cttcctggcg cgtcgtcgcc acctacaggc gctggaacag
    44161 gcagcggagc atttgcagca aggtaaagcg caactgttag gtgcctgggc gggtgaactg
    44221 ctggcggaag agttgcgcct ggcacagcag aacttaagcg aaatcaccgg ggaatttact
    44281 tcagacgacc tgctggggcg gattttctcc agcttctgta ttggtaagta accgcgctta
    44341 cgaagccgta ttctgactgt cagatgcggc ttcgcttcat tgttagcact cctgttattc
    44401 ctcaaccctt tttttaaaca ttaaaattct tacgtaattt ataatcttaa aaaaaagcat
    44461 ttagtattgc tccccgaacg attgtgattc gattcacatt taaacaattt tagaataga
//
LOCUS       DAZGSP010000035        43154 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZGSP010000035 DAZGSP010000000
VERSION     DAZGSP010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.035"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            256..987
                     /gene="artJ"
                     /locus_tag="ACHK74_002054"
     CDS             256..987
                     /gene="artJ"
                     /locus_tag="ACHK74_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFP0220877.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(1162..2289)
                     /gene="rlmC"
                     /locus_tag="ACHK74_002055"
     CDS             complement(1162..2289)
                     /gene="rlmC"
                     /locus_tag="ACHK74_002055"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFP0220878.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(2330..2818)
                     /gene="ybjO"
                     /locus_tag="ACHK74_002056"
     CDS             complement(2330..2818)
                     /gene="ybjO"
                     /locus_tag="ACHK74_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFP0220879.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(2878..3723)
                     /gene="potI"
                     /locus_tag="ACHK74_002057"
     CDS             complement(2878..3723)
                     /gene="potI"
                     /locus_tag="ACHK74_002057"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFP0220880.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(3720..4673)
                     /gene="potH"
                     /locus_tag="ACHK74_002058"
     CDS             complement(3720..4673)
                     /gene="potH"
                     /locus_tag="ACHK74_002058"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFP0220881.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTLCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(4683..5816)
                     /gene="potG"
                     /locus_tag="ACHK74_002059"
     CDS             complement(4683..5816)
                     /gene="potG"
                     /locus_tag="ACHK74_002059"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFP0220882.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(5911..7023)
                     /gene="potF"
                     /locus_tag="ACHK74_002060"
     CDS             complement(5911..7023)
                     /gene="potF"
                     /locus_tag="ACHK74_002060"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFP0220883.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(7375..7851)
                     /gene="ybjN"
                     /locus_tag="ACHK74_002061"
     CDS             complement(7375..7851)
                     /gene="ybjN"
                     /locus_tag="ACHK74_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFP0220884.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(7939..8841)
                     /gene="rimK"
                     /locus_tag="ACHK74_002062"
     CDS             complement(7939..8841)
                     /gene="rimK"
                     /locus_tag="ACHK74_002062"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFP0220885.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(8902..9624)
                     /gene="nfsA"
                     /locus_tag="ACHK74_002063"
     CDS             complement(8902..9624)
                     /gene="nfsA"
                     /locus_tag="ACHK74_002063"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFP0220886.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQSLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(9608..9895)
                     /gene="ybjC"
                     /locus_tag="ACHK74_002064"
     CDS             complement(9608..9895)
                     /gene="ybjC"
                     /locus_tag="ACHK74_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFP0220887.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            10055..10312
                     /gene="grxA"
                     /locus_tag="ACHK74_002065"
     CDS             10055..10312
                     /gene="grxA"
                     /locus_tag="ACHK74_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFP0220888.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(10342..10719)
                     /gene="ybjM"
                     /locus_tag="ACHK74_002066"
     CDS             complement(10342..10719)
                     /gene="ybjM"
                     /locus_tag="ACHK74_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFP0220889.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            10989..12674
                     /gene="ybjL"
                     /locus_tag="ACHK74_002067"
     CDS             10989..12674
                     /gene="ybjL"
                     /locus_tag="ACHK74_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFP0220890.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(12849..13385)
                     /gene="rcdA"
                     /locus_tag="ACHK74_002068"
     CDS             complement(12849..13385)
                     /gene="rcdA"
                     /locus_tag="ACHK74_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFP0220891.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            13469..14677
                     /gene="ybjJ"
                     /locus_tag="ACHK74_002069"
     CDS             13469..14677
                     /gene="ybjJ"
                     /locus_tag="ACHK74_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0220892.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14677..15492
                     /gene="ybjI"
                     /locus_tag="ACHK74_002070"
     CDS             14677..15492
                     /gene="ybjI"
                     /locus_tag="ACHK74_002070"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFP0220893.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            15578..15862
                     /gene="ybjH"
                     /locus_tag="ACHK74_002071"
     CDS             15578..15862
                     /gene="ybjH"
                     /locus_tag="ACHK74_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFP0220894.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(15903..17135)
                     /gene="mdfA"
                     /locus_tag="ACHK74_002072"
     CDS             complement(15903..17135)
                     /gene="mdfA"
                     /locus_tag="ACHK74_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFP0220895.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTISAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            17420..18016
                     /gene="ybjG"
                     /locus_tag="ACHK74_002073"
     CDS             17420..18016
                     /gene="ybjG"
                     /locus_tag="ACHK74_002073"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP0220896.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            18074..18832
                     /gene="deoR"
                     /locus_tag="ACHK74_002074"
     CDS             18074..18832
                     /gene="deoR"
                     /locus_tag="ACHK74_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFP0220897.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(18879..20081)
                     /gene="dacC"
                     /locus_tag="ACHK74_002075"
     CDS             complement(18879..20081)
                     /gene="dacC"
                     /locus_tag="ACHK74_002075"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP0220898.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            20328..20954
                     /gene="gstB"
                     /locus_tag="ACHK74_002076"
     CDS             20328..20954
                     /gene="gstB"
                     /locus_tag="ACHK74_002076"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFP0220899.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(20951..22066)
                     /gene="yliI"
                     /locus_tag="ACHK74_002077"
     CDS             complement(20951..22066)
                     /gene="yliI"
                     /locus_tag="ACHK74_002077"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFP0220900.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQVGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRSGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(22177..22560)
                     /gene="bssR"
                     /locus_tag="ACHK74_002078"
     CDS             complement(22177..22560)
                     /gene="bssR"
                     /locus_tag="ACHK74_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFP0220901.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            22773..24098
                     /gene="rimO"
                     /locus_tag="ACHK74_002079"
     CDS             22773..24098
                     /gene="rimO"
                     /locus_tag="ACHK74_002079"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFP0220902.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(24145..25473)
                     /gene="dgcI"
                     /locus_tag="ACHK74_002080"
     CDS             complement(24145..25473)
                     /gene="dgcI"
                     /locus_tag="ACHK74_002080"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP0220903.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECPTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(25481..27829)
                     /gene="pdeI"
                     /locus_tag="ACHK74_002081"
     CDS             complement(25481..27829)
                     /gene="pdeI"
                     /locus_tag="ACHK74_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0220904.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSGGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(28007..28918)
                     /gene="gsiD"
                     /locus_tag="ACHK74_002082"
     CDS             complement(28007..28918)
                     /gene="gsiD"
                     /locus_tag="ACHK74_002082"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFP0220905.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIAVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(28921..29841)
                     /gene="gsiC"
                     /locus_tag="ACHK74_002083"
     CDS             complement(28921..29841)
                     /gene="gsiC"
                     /locus_tag="ACHK74_002083"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFP0220906.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(29859..31397)
                     /gene="gsiB"
                     /locus_tag="ACHK74_002084"
     CDS             complement(29859..31397)
                     /gene="gsiB"
                     /locus_tag="ACHK74_002084"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFP0220907.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKVQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(31417..33287)
                     /gene="gsiA"
                     /locus_tag="ACHK74_002085"
                     /pseudo
     CDS             complement(31417..33287)
                     /gene="gsiA"
                     /locus_tag="ACHK74_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
     gene            complement(33274..34239)
                     /gene="iaaA"
                     /locus_tag="ACHK74_002086"
     CDS             complement(33274..34239)
                     /gene="iaaA"
                     /locus_tag="ACHK74_002086"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP0220908.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            34443..35678
                     /gene="moeA"
                     /locus_tag="ACHK74_002087"
     CDS             34443..35678
                     /gene="moeA"
                     /locus_tag="ACHK74_002087"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFP0220909.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            35678..36427
                     /gene="moeB"
                     /locus_tag="ACHK74_002088"
     CDS             35678..36427
                     /gene="moeB"
                     /locus_tag="ACHK74_002088"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFP0220910.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(36503..37165)
                     /gene="fsa"
                     /locus_tag="ACHK74_002089"
     CDS             complement(36503..37165)
                     /gene="fsa"
                     /locus_tag="ACHK74_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP0220911.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            37296..38195
                     /gene="ybiY"
                     /locus_tag="ACHK74_002090"
     CDS             37296..38195
                     /gene="ybiY"
                     /locus_tag="ACHK74_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFP0220912.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            38201..40633
                     /gene="ybiW"
                     /locus_tag="ACHK74_002091"
     CDS             38201..40633
                     /gene="ybiW"
                     /locus_tag="ACHK74_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFP0220913.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            40779..41594
                     /gene="ybiV"
                     /locus_tag="ACHK74_002092"
     CDS             40779..41594
                     /gene="ybiV"
                     /locus_tag="ACHK74_002092"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFP0220914.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            41746..43011
                     /gene="ybiU"
                     /locus_tag="ACHK74_002093"
     CDS             41746..43011
                     /gene="ybiU"
                     /locus_tag="ACHK74_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFP0220915.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT        10817 a        11410 c        10555 g        10372 t
ORIGIN      
        1 ctgataagac gcgccagcgt cgcatcagac atcagcacgg tgcctgtgcc ggatgcggcg
       61 tggacgcctt atccggccta catagtgcat aaattcttat aatgaagacg gacaactcac
      121 taaggtatcc gtcttttttt atttcattta aattatttaa tcatatttat tgcatataaa
      181 ttcacttgat ggcattgtta tcccatgccg cagacacggc caaaaatcat aagattgaca
      241 gacgggagtt ccatcatgaa aaagttagtt cttgccgctt tacttgcttc ctttactttc
      301 ggtgcttctg ccgcagagaa aatcaatttt ggcgtttcag ccacctatcc accctttgaa
      361 tctataggtg ctaataatga gattgtcggc tttgatatcg atctggcaaa agccttgtgc
      421 aaacaaatgc aggcagaatg tacttttact aatcacgcgt tcgacagcct gatcccgtca
      481 ctgaaattca gaaaatatga cgccgtaatc tccggtatgg atatcacgcc cgagcgtagc
      541 aagcaggtat cgtttaccac gccctactat gaaaactcag ccgtcgtgat tgccaaaaaa
      601 gatacctaca aaacgtttgc cgatctgaaa ggcaaacgta ttgggatgga aaacggcacc
      661 acgcaccaga aatatattca ggatcagcac cccgaagtga aaactgtctc ctatgacagt
      721 tatcagaatg cctttatcga tctgaaaaat ggtcgtattg atggggtatt tggtgacaca
      781 gcggtggtaa acgaatggct gaaaaccaat ccgcaactgg gcgttgcgac tgaaaaagtg
      841 accgatccgc aatacttcgg caccggcctg ggtatcgctg tacgtccgga taacaaagct
      901 ctgctggaaa aactgaataa cgcgctggca gcaattaaag ctgacggtac atatcagaaa
      961 atcaatgacc agtggttccc gcagtaattg agactgatga caaaagtaag attgcctgat
     1021 gcgctgcgct tatctggccc acatcaactc tgcaatgtat tgatttagca tgattttgta
     1081 ggccggataa ggcgtacacg ccgcatccgg catgaacaaa gcgcacctga tcagcaatct
     1141 gaagccgcac ctgcggcttt tttattgctt caccagcagc gtcatcactt catagtgcgc
     1201 ggtgtgcggg aacatatcaa aaagctgtac ccgttcgatg cggtaacctg gcagttcgcg
     1261 gatatctttc gccatggttt gggcgttaca gctggagtag ataataaaac gcggtgccat
     1321 cgttgagaga taatcacaca gcggtttacc aatgccgcgg cgcggcgggt taaccagcac
     1381 cagctccggc acatccccct gagcggtggc aaactgagtg gagtccagcg cctgaaattg
     1441 cagacgcgtt aaacccagtt cagcggctga ctgctttgca caggcaatgg cctctggcgc
     1501 aatttcgatc ccggttaact gcatgtcagg cgtcgcgcag tgtaaaccaa agccccccac
     1561 accgcagaac agatcccaca tatgtttaac cggcagctgt cgtacccagt cgcgcgcggt
     1621 ggcgtacaac tggctggcga ccgccggatt cgtctggaag aaactttgcg gacggatcca
     1681 cagcggtacg tcattaaaac gctctgccag tgcctgttgt tcggtcaggt agatctccgt
     1741 ctccccttcc ataatcgcca tatgtaccgg ctgaatattg acggtaataa ctttcagctg
     1801 cggtagttgt tcgtgtaacc acggcagcgc cttacgcagt tgcgccagct tggtatcaga
     1861 acgcagtaca aagcgcagca tcatgccgcc atcactctgg ctttcagtca gcagaatgta
     1921 tttcagttcg ccacgcttac gcgccacgtt gtagggcgtt aaccccgctc gggcaataaa
     1981 cggttttagc gccgcaaaaa cgggcgcaaa tgaggcagga taaagcgggc agtcacaaag
     2041 gtcttctggt gtgccgtcgc gatgcagcat accgagcagt ggtttttcaa cgctaccact
     2101 caccaccatt ttggccttat tacgaaaccc ttgttccggg cctgacaccg gcgcgcacca
     2161 ttcctcaacc ggaaagtcgg cgagcagatt tttaagatcg gcggttttag cggagagttg
     2221 ctctggaatc ggctgcgtta tccactgaca ggaacgacag cgacccgcgt cgtaaagtgc
     2281 gcactgcata cattgacctt cacatcatca ggggcgacga ttatacacat tattgcaact
     2341 ggaagaaccg ccgactggtc gagggaacga acagcagcat caggatgagc atatccggca
     2401 gcttctgcag catcaggcta tggaagattt cacgttttga ttcaccggga atgctgaaca
     2461 gctccggata accgtacccc agcgaagccg cccacaagta acttgcggcg gtgatttgtg
     2521 tcagcagata tagccagcgc gcccagcgac gccctttcac cagtgaaaac gcacaccaga
     2581 tctcaatgaa aaccagcacc agactgctta aaaagaccag cgttaaactc caggtttgta
     2641 cgctgcgatg aatgaactcg ccaataccgc gcacgcccag cgtattgaaa atcatcagca
     2701 cgtcgaggcc acggatcata ataatggcga gcgccgccac ctgcaccagc gcaggcacat
     2761 tcaggcgagc atgagatgaa gatgttttct taaaaaatcc caacgtttcg tcttccatga
     2821 aaacaatgcc gcgacatgcg cggcattatg tagccaggtt ggcaaatttt agtgtcttca
     2881 gccacgtctt gcacgctgga tgtcgcgtat ccgctgtttt tctgcgcgag ccatcagata
     2941 ccaggcgata aatccgacaa ttccgaccgc gccgaggatc aatgttgcca gggcgttgat
     3001 ttccggattc acccccatcc gcacgctgga aaagaccagc atcggtaacg tggtggctcc
     3061 cggcccggaa acaaagctgg cgatcaccag atcatcaagc gacaaagtaa aagccagtaa
     3121 ccagccagaa atgatcgctg gcatgatcat cggtagcgta atgacgaaaa acactttcag
     3181 cggcgtcgca ccgagatcca tcgctgcttc ttctatcgag cgatccagtt cccgcaatct
     3241 tgaagaaata acgaccgcca cataagccgt acaaaaagtg acatgcgcca gccagatggt
     3301 gagcataccg cggtccgcag gccagccaat ggcatgagca agcgcgacga ataataacaa
     3361 cagcgacaag cccgtgatga catctggcat taccagcggc gcggtgatca taaaggcaaa
     3421 gccatttgat ccgcgaaacc tgccaaagcg caccagcacc accgccgcaa tagtcccgag
     3481 gatcgccgcc gccgttgccg cacaggcagc aattgtcagg cttaaaccaa ccgcactcat
     3541 catcgcgtca tcgcgcaata actcaccata ccagcgcgtt gaccagccgg cccacaccgt
     3601 caccagcttc gagctgttaa acgaatagat aaccagcatc agcattggcg cgtagagaaa
     3661 ggtaaagccc agcagcaaaa tcacaatccg ccagggtgaa cgaactaccg gtaaattatt
     3721 catccgtgtt ctcccacgct tttttgctgg tgtttgtgga accacattat cggcacaatt
     3781 agcagcagca acatgatgat cgctaccgcc gaggccaccg gccagtcgcg gttattgaaa
     3841 aactcttgcc atagcacgcg cccgatcatg atgctgtccg ggccaccgag cagttccggg
     3901 atcacaaact cgcccacagc cgggataaac accagcatcg atccggcaat aatcccacct
     3961 ttagtcagcg gcacgatcac agtaaagaac gttttcagcg gtcgtgcacc gagatccagc
     4021 gctgcttcca ccagcgaata atcaatacga atcaacgcgg tataaatcgg cagtaccata
     4081 aacggcacgt aagcgtaaac aatgccgata taaacggcca gattggtatg cagaatggtc
     4141 agcggttgat cgataacccc cagccacagc agaaaattat tcagcacacc gttgtttttt
     4201 aatattccca tccaggcata aacgcggatc agaaacgagg tccacgacgg caggatcacc
     4261 agtagtaata agatattacg ggtcgagggc ttactgtgcg ccaccgccca cgccagcgga
     4321 tagccgatca gtaaacagca aagtgtcgaa atcgccgcca cttgtaacga ctggagataa
     4381 gcatcgaaat agagcggatc gtcagtcagt tgcagaaaat taccgagatt aagagtgatg
     4441 gacagttgcc cgtcagccca ctccatcagt tcggtataag gtggaatagc gcgcgccatc
     4501 tccgccaggc ttattttaaa gacaatcaga aatggcagca gaaacagcaa gatcaaccag
     4561 atatatggca acgcaatgac cagtttgcgc ccatgcttca tttgcagctg cgacaaccac
     4621 agcttaaatc cgcccggcgg tttcgactgg gcagcaggtt caagtgtatt catctttgct
     4681 ccttaaaccg tcagcaccac acagctgtcc acttcccagc acaaacgcac ttcgtcgccc
     4741 caggtcggta accctttacg atgacgatgg gcgttttgta gctgggcgct aatcatctgc
     4801 ccacttttca gacgaacgtg atacaccgaa agatcgccga gataggcaat gtgtatcacc
     4861 tcccccaccg cgaagttaca accattggcg ggcggctctt cgcaaagcat gattttttcc
     4921 gggcgcagcg ctacgtgtac cggcacgtta tcgaccactg aagcatccgc atcgactttc
     4981 agtggatgca ccagccccgg cgaatcaagc accaggccat cttcctgacg ctctttgagt
     5041 acaccttcaa agacattaac cgaaccgata aattcagcgc tgtagcgggt agttggatgc
     5101 tcgtagatct cttccggctc gccaatctgg acaaatttcc cgcgattcat aatggcgata
     5161 cgccccgcca tggtcatcgc ctcttcctga tcgtgggtga ccatcacaca agtcacaccg
     5221 acgcgctcca gaatatccac cacttcaagc tgcatccggt cacgcagctt tttatccagc
     5281 gcgcccatcg gctcatcgag cagtaatagt ttcgggcgtt tggcaaggct tcgggccagg
     5341 gccacacgct gtcgctgacc accggaaagc tgatgcggtt tgcgtttggc gaactcctgc
     5401 atgtgcacca ggccgagcat ctcatggacc cgactggcaa tttccgcttt cggtagtttg
     5461 tcctgtttca ggccaaaagc gatgttctgt tccacggtca tatgcggaaa cagcgcgtaa
     5521 gactgaaaca tcatattgat ggggcgcagg taaggcggca cctgtgacaa atcgacgcca
     5581 tcaagcatta tctgtccggc agaaggttgt tcgaaacctg ccagcatacg cagcagcgtg
     5641 gacttgccac agccggatgc gcccagcagc gcgaagattt cacctttgta gatggtcagg
     5701 ctgacatcat ccaccgcatg ttgaccatcg taggatttgg tgaggttgcg gatttctaat
     5761 agcggcgtca gcgccttacg ggttttcgcc tgcgggcgag ggatagcgtc attcacggct
     5821 gctctccggc attgaatgtg tgcaagcgca ccatgatggt gcgaattgcc ggcgggtgtg
     5881 gtgcgcccgc gcggcatcta aggctgcgga ttattttccg ctcttcactt tggtccacgc
     5941 gcgggtgcgc acacggtcga ttttcgggtc ctgcactttc agagtgaaca gcttcgcacg
     6001 aacatccgca ggcggataaa tacccgggtt ctcacggact tccgcactca ccagcggcgt
     6061 ggctgcttta ttggcgttgg catagaacac atggtcggaa atatgcgcta ctacatccgg
     6121 gcgcagcagg taattcagga actgataggc ttcgtctttg tttttggcat ccgcaggcat
     6181 ggcgaataca tcaaagaacg ccatcgcccc ttcttttgga atcgagaacg agacattaac
     6241 gccattcttc gcttccttcg cgcggtttga cgcctgccag acatcgcccg cccagccaat
     6301 agcgacgcag atatcgccgt ttgccaggtc gttaatgtat tgagatgaat ggaaataacg
     6361 aatgttcggg cgcagcttta acagcagatc ggtagccggt ccggtgtaat catccgcttt
     6421 agtgctgttg ggatctttgc cgagataatt cagcacggta gcaaaaactt cttctggcgc
     6481 atccaggaaa gagacaccgc agcttttcag tttttccaga ttttcaggct tgaggatcaa
     6541 gtcccagcta tcgacgggcg cgttttcgcc cagcacagct ttaactttat caacgttata
     6601 gccaatcccg gtcgtcgccc acatataggg catagcaaat ttattgtcgg gatcgtgttt
     6661 ggcgaccaac ttcagcagct ccggatcgag attcttccac tccggcaatt tgcttttgtc
     6721 cagcggctgg aaaactcccg ctgtcagctg gcgttcaaga aagctggcag atggaaccac
     6781 caagtcaaag ccggtgctac ccgccattaa tttgccttcc agtacttcgt tagagtcgaa
     6841 aacatcgtag acgactttaa taccggtttc tttttcaaaa ttggccaccg tgtcaggggc
     6901 gatataatca gaccagttat aaatgtggag tgttttttgt tcagccgcga gcgtgccgac
     6961 agagacggcc atcagagcac ccgcaaccag acccgatagc cattttttat ttaaggcggt
     7021 catatctctt tccttctgaa agttcgttaa caaacgaaca aaagttgtca cactgaaaga
     7081 tatgcaataa acgtgcatat ttttttatcg gcgcggtgta agaaaagaga actctctccc
     7141 ggccagcact gctcgctata taaaagcatc gcaagaataa ctttagccag ggtttgagac
     7201 tatagctcgg attaaaaaac ggtatttaac aaatttttat gcgtatttgc tctatgtgat
     7261 aaagccaaat cgacacaaga taagtgaaat aatctttaag aaaaggttaa aaacagcaga
     7321 taattttgca gcgcgcagtc actgcggcag cgactgcgtg ctcgaaagga attatcagtg
     7381 gagaaaatag ttttcggtaa cgttgttttg cccttcatcg tcagtcggta acagcagttg
     7441 atgggcatta gcttcaagaa tgaccatcga aatctgctct tcgctctgac gcacgaacca
     7501 tgcaaactgc tcataggtta cgccctgcat aacggataaa gactggcaaa ccaccagctt
     7561 tggcagatta tcatcctgca tatcaagaaa tgctttcacg gtcagcgaac tggcattgat
     7621 ggcagataaa tccgccgcca gcggcaatac ggctgaaggt cggacttccg ccatggcaga
     7681 aaacaggatc gtgttatcga tcagatcgat tttggcatca aagacaccgt cgaaattctg
     7741 catatggggc agatgcagag cctgacagtt atcacattca aaaaaactca tccccaggtc
     7801 atcgagccat tgacgcagcg tatccagacc aggaacgacc agcgatgtca taattttgta
     7861 caacctcttt cgataaaaag accggcacag cttacgcaaa aagcgcaggc aaaaccatga
     7921 tcagtaatgt gattgcgact aaccacccgt tttcaggcaa tattctgtcg tagcgtggcg
     7981 ttcgatccag cggatcattt tacccgcgat gtcgataccg gtggtttttt ctattccttc
     8041 cagccccggc gacgcattca cctccatcac caacggcccg cgattagcac gcagaatatc
     8101 cacaccagca acgtccagcg ccatcgttcg cgcggctttt atcgcgattt cacgctcctg
     8161 tggtgtgata ctggcgacgc ttgccgcgcc gccacgatgc aaattggaac gaaaatcgcc
     8221 ctctttcgcc cgccgttcaa tcgcagcgac cacttcatcg ccaacaacca gacagcggat
     8281 atcgcaccct tgcgcctctt tgatatattc ctgcaccaga atatgcgcgt tcagaccgcg
     8341 gaaagcgtca atcacgcttt ccgccgcctg acgcgtctcc gccagcacga cgccaattcc
     8401 ctgcgtgcct tcaaccaact tgaccaccag cggcgcacca ccgaccatgt cgattaaatc
     8461 gctggtatca tccggcgaat gcgcaatgcc cgtgacaggc aggtcgatgc cctgacgcgc
     8521 cagcagttgc atggaacgca atttgtcacg cgcccgggca atggcgaccg actcattgag
     8581 cggatagctc cccagcatct cgaactggcg cagtgccgcc gtcccataaa aggtaatggc
     8641 ggtgccaata cgcgggatca ctgcgtcaaa atggggtaac ttgcggcctt tgtagtgaat
     8701 agaagacgcc gcaggattta tgttcatgta gcaagaaagc ggatcaagaa tttcaaccag
     8761 gtgaccgcgc tgtatagcgg cttcacgcag ccgcttacac gaatagagcg ttccatcccg
     8821 ggacaatatg gcaattttca ccctgcacct ctctgtcaga cctggtcaaa agcccgcgta
     8881 tcatacatcg acgtggcggt tttagcgcgt cgcccaaccc tgtttgtgca aataatccag
     8941 aataaatggg cggctttctt taatgattgt tcggcggata tgatcgctcc aggtatcccg
     9001 gcgattattg ctgccacggg tgaggtaata ttccgccagt tgctcgtcat actgcgccag
     9061 tgcgccttta tccagcgatt gatagctgtt ttcatgcacc aaaatggagg ccggtaaacg
     9121 cggcttaaga tccggattat ccgcaggcca gccaaggcac agcccaaaca gcggcagaac
     9181 atgctgcggt aatttaagca gtttcgtcac cgcttcaata ttattgcgca ggccgccgat
     9241 atataccccg cccaatccca gcgattccgc tgcgattaat gcattctgcg ccatcattgc
     9301 cgtatcaacg acaccgagca acagttgttc cgccaggccg agctgagcat ccggacagat
     9361 ctgtaaatgg cggttaaagt cggcacagaa cacccagaac tccgccgctt gcgctacgtg
     9421 tttttgcccg ccggtcagcg tcaccagttc ttcacgtaac gctttgtcgg taatgcgaat
     9481 aatgctactg cactgcaaaa aactggaact ggacgtcgca cgggcgctgt taataatcgc
     9541 ctcacgctgc gcttcggaaa tgggttcatc agtgaaatgg cgaatggagc gatggccaca
     9601 aataagttca atggttggcg tcattatctt tttctctttc tgaacgtgaa tattgcggtg
     9661 gacggttcat cagctgtggg gcaagacgtt ttgccacctg aagaataacc accaccgcag
     9721 cgggaagcat gagcaaaaca ccgagaaaaa tcatcagaat ctgcacttct ggccgagaaa
     9781 atggctcagg cagcgacagg gagtcgctta ccgacagcag cgccaccgcc agtagcatca
     9841 ttccgataaa ttccagtatc aacacgcctt taggcaattt accgatcgcg cgcatacgct
     9901 tccctctgca aagtgagcct tcagtctaaa acttttcact gtattgtgtt taacagttat
     9961 agcttttagc aattaatgca acaggttaaa cctactttca gcgaatacat tttagcgtga
    10021 tcattacagg cataaatcta tgaggagaga aataatgcaa accgttattt ttggtcgttc
    10081 gggttgccct tactgtgtgc gtgcaaaaga tctggctgag aaattgagca atgaacgcga
    10141 tgattttcag tatcagtatg tagatattcg tgcggaaggg atcactaaag aagatctaca
    10201 acaaaaggca ggtaaacccg tagaaaccgt gccgcagatt tttgtcgatc agcaacatat
    10261 cggcggctat accgattttg ctgcatgggt gaaagaaaat ctggacgcct gatcgtctga
    10321 caagccctcg cgttgagggc tttactgatt ttttctgtgc tgtggtttaa acaaactact
    10381 gataaataag aaacacagtg cccccagcgc acaccagaac accgcgctta gtaaccatgc
    10441 cagctcttgc cagaatgagc gcgtcggtga aaaaaacagc cgcataatga gcattgaaca
    10501 gggtgccgcc agcattgcgc caaatagagg tttcaggact tctctacgct gtgaaaagaa
    10561 actggcaact gctccaggaa gaatgaaaaa tagcaagccg atttcaggat gcccggcagc
    10621 ccgaaaagcg cctttcatgt gcgtcgccag aaaaaggcac accacaatga agaggacaaa
    10681 acagcagatt gcccccgccc aacgttgttt atgtttcact cgttcctcct gacactgcgt
    10741 ctatcgaaca catttttcgc cagtgtggcg ttcagtaaga taaagccgct tcgcattcca
    10801 tgctaatata ggccaacgca attcatatag ccgttgatac ctaatgtgat tacactagta
    10861 aaatatattg ttactttact atcgtttagg tgcgctgaat gaatctgcgc cctgaattct
    10921 ggtaaaaaac attatcgtaa attaccattt ctttcaacag cttactagta aacaagaagt
    10981 tagcctccgt gaatataaac gtcgccgaat tgttaaatgg gaattacatt ctgttattat
    11041 ttgtggtcct cgcgcttggg ctatgtctcg gaaagttacg acttggttcg atccaactgg
    11101 gtaattccat tggcgtttta gtcgtatcgc tgttattagg ccaacaacat ttcagcatta
    11161 acaccgatgc gcttaatctt ggctttatgc tgtttatttt ctgcgtcggg gtcgaagccg
    11221 gaccgaactt tttttccatt ttttttcgcg atgggaaaaa ttacctaatg ttagcactgg
    11281 tgatggttgg cagtgcgctg gtgatcgcct tagggttagg taagctgttt ggctgggata
    11341 ttggcctgac ggccggtatg ttagcaggct ctatgacgtc gacaccggtt ctggtcggtg
    11401 ctggcgatac actgcgtcat tccggcatgg aaagcaggca gctctcactg gcactggata
    11461 atctgagcct cgggtatgcc ttaacctatt taatcggtct ggtgagtttg attgttggtg
    11521 cgcgttactt gccgaaattg cagcatcagg acttacagac cagcgcccag caaatcgccc
    11581 gcgaacgtgg cctggacact gatgccaacc gtaaggttta tttaccggtg atccgcgcct
    11641 atcgcgtcgg cccggagctg gtggcctgga ccgacggcaa aaatctgcgt gaactgggta
    11701 tttatcgaca aaccggctgc tacattgaac gtattcgacg taacgggatt ctggcaaatc
    11761 cagacggtga tgccgtgcta caaatgggcg atgaaatagc gttggtaggc tatcccgacg
    11821 cccatgcccg actcgatccc agcttccgta acggtaaaga agttttcgat cgtgaccttc
    11881 tcgacatgcg tatcgtcact gaagaagtgg tcgttaaaaa ccataacgct gtaggtaaac
    11941 gtctcgcaca actgaagttg accgatcacg gttgcttcct taaccgcgtc attcgtagcc
    12001 agattgagat gccgatagat gacaacgtcg tgcttaacaa aggtgacgtt ttacaagtca
    12061 gcggtgatgc ccgtcgcgta aaaaccatcg ccgatcgcat cggctttatc tcgattcaca
    12121 gccaggtcac tgacctgctg gcattttgcg ccttctttgt tattgggctg atgatcggga
    12181 tgatcacctt ccagttcagc acattcagtt tcggcatggg gaacgctgcc gggttgttat
    12241 tcgccggaat tatgctgggc tttatgcgtg ctaaccaccc gaccttcggt tacattccgc
    12301 aaggtgcatt aagcatggtg aaagagttcg gcttgatggt gtttatggca ggcgttggtc
    12361 tgagcgccgg tagcggtatt aataacggcc tgggcgcgat tggcggtcag atgttgattg
    12421 ccggattaat tgtcagtctt gtgcccgtgg ttatctgttt cttgttcggt gcttatgtat
    12481 tgcgaatgaa ccgcgcactg ttgttcggcg caatgatggg cgcacgcacc tgcgcgccgg
    12541 caatggagat catcagtgat acagctcgca gtaacatccc tgcgctgggc tatgcgggca
    12601 cctacgcaat cgccaacgtc ctgctgacgc tggcagggac aatcatcgtc atggtatggc
    12661 caggattagg ataaaactga agttgccctg aaaatgaaat ttttttgcac aaccgcagaa
    12721 cttttccgca gggcatcagt cttaattagt gccactgctt ttctttgatg tccccatttt
    12781 gtggagccca tcaaccccgc catttcggtt caaggttgat gggttttttg ttgtctaaaa
    12841 cttatctact accctgcaac cctctcaacc atcctcaaaa tctcctcgcg cgatagcggc
    12901 ttacggtcgg tgacaaaatg cagcgtcatc ccctcaataa acgcatcaag cgcgcgggcg
    12961 gttccgggtt caaaccattg ttcgagcgtt tgctgactgc gctgcatcca gttttgcatt
    13021 accgttttta atagcggttt tcggctagcc agcgcgtaga gctggtacat cagctccata
    13081 ttatccggcg ttgcaacctg tgagctgtag atcatatcgg tgatagcctg gcatgcgcct
    13141 tgagcatcac taacatcgct aaaaaatgcc tgatattgcc gggacataat ctcagtaaaa
    13201 ctgctgaacg cctccagcaa caactcatca attcctgaaa aatagtaggt catcgatccc
    13261 aacggtaccc cggcaagggt agcaattttg cggtgcgtaa cagcatgtat tccgtaaagt
    13321 ttcaccgcct ccagcgtggc ctggataatt ttttctcgcc gttgcggatc gttagcgcga
    13381 cgcatgtcat ttcctctctc tgcaattgtg tacaaatgta cacaaccttg ctaatgttgt
    13441 cttccctctt cttattttga cgtgatctat gaccgtaaat tcttcacgta atgcattgaa
    13501 acgccgaacc tgggcgctgt ttatgttctt ctttttgcca ggcctgttaa tggcgtcctg
    13561 ggcaacccgt acgcctgcta tccgcgatat tctctctgtc tcgatcgctg aaatgggcgg
    13621 tgttctcttt ggtctgtcga tcggttcgat gagcggtatt ctctgctcgg cgtggttagt
    13681 gaaacgcttt ggcacgcgta atgtcatcct ggtcacgatg tcctgcgcat tgatcgggat
    13741 gatgatacta agtctggcac tctggctgac atcgcccctg ctcttcgcgg ttggtctcgg
    13801 cgtctttggg gcaagttttg gttctgcgga agtggcgata aacgttgaag gtgctgccgt
    13861 tgagcgggaa atgaataaga cggttttgcc gatgatgcac ggtttttata gcctgggcac
    13921 gctggcaggc gctggtgtcg ggatggcact gacggccttt ggcgttccgg caacggtgca
    13981 cattttattg gcggcgctgg taggcatcgc gcctatttat atcgccattc aggcaatccc
    14041 tgacggtacg ggcaaaaatg ctgccgatgg cacccagcat ggcgaaaaag gcgtaccttt
    14101 ttatcgcgat atccagttgc tgctgattgg tgttgtggtg ctggcgatgg cctttgccga
    14161 aggttctgcc aacgactggt tacccttatt aatggttgat ggtcacggtt ttagccccac
    14221 ttccggctca ctgatttatg ccggttttac cctggggatg accgttggac gcttcaccgg
    14281 cggttggttc atcgaccgtt acagtcgcgt tgccgtggtt cgggccagtg cactaatggg
    14341 ggcgttgggt attgggctga ttatttttgt cgatagcgcc tgggtcgcag gagtgtctgt
    14401 tgtactttgg ggactgggtg cctcgctggg cttcccgctg accatttctg ccgccagcga
    14461 taccggcccc gatgcaccaa cccgcgtcag tgtggtagca acgaccggtt atctggcttt
    14521 cctcgtcggg ccgccgctgc tgggctatct cggcgaacat tatggattac gtagtgcaat
    14581 gctggttgta ctggcgctgg ttattctcgc ggctattgtc gcgaaagccg tcgccaaacc
    14641 cgataccaaa acgcagacgg cgatggagaa tagttgatga gcattaaatt aattgcggta
    14701 gacatggatg gtactttctt aagcgatcaa aaaacctata accgtgagcg gtttatggct
    14761 cagtatcagc aaatgaaagc acaaggcatt cgctttgtgg tcgccagcgg gaatcaatat
    14821 tatcagttga tctctttctt ccctgaaatt gctaatgaaa ttgcctttgt ggctgaaaac
    14881 ggcggctggg tagtgagcga aggcaaagat gtttttaatg gcgagctgtc gaaggatgcg
    14941 tttactactg tcgtggaaca tttgctgacg cgcccggaag tggaaattat tgcctgcgga
    15001 aaaaatagcg cctatacgct caaaaaatat gacgatgcca tgaaaacggt ggcggaaatg
    15061 tattatcacc gtctggaata cgtcgataac tttgacaact tagaggatat cttctttaag
    15121 tttggcctga atctttccga tgaactgatt ccacaagtac aaaaagcatt acatgaggcc
    15181 atcggcgata ttatggtgcc ggtccacacc ggcaacggca gcatcgatct gattatcccc
    15241 ggcgtacata aagccaatgg ccttcgccaa ctgcagaaat tatggggaat agacgacagc
    15301 gaagtggtgg tctttggcga tggcggtaac gatattgaga tgctgcgtca ggcaggcttt
    15361 agttttgcaa tggaaaatgc cggcagcgcg gtcgtcgcag cggcaaaata ccgggcaggc
    15421 tccaataacc gtgaaggcgt actggatgtg atcgataaag tgcttaaaca cgaagcgcca
    15481 tttgaccaat aaaaacggca gcacgcccgg caacacaata cctgaatgaa ggctgcataa
    15541 ttcgcggaaa ttaacccgct gcattatttc aggcgaaatg attatgaaaa attgtctact
    15601 gttgggcgcg cttttaatgg gctttactgg tgtggcgatg gcgcaaagtg tcaccgtcga
    15661 tgtgccgagt ggatacaaag tggttgtagt tcctgattca gtcagcgttc cacaagcggt
    15721 cagcgtcgcg actgtgccgc aaaccgtcta tgtggctcct gcgccagctc cggcttaccg
    15781 cccgcatcct tatgctcgcc atcttgccag cgtaggtgaa gggatggtta tcgaacacca
    15841 gattgacgac catcaccatt aattcgagaa tgcctgatcg cacaaagcag tcaggcattt
    15901 ttttaccctt cgtgagaatt tcccatctgt ttatctttta aaaagataac catcagcgac
    15961 agccacaaaa ttccgttgac aaggttgaag agattaaact gtccgttgcc cccgttcagc
    16021 caggcatgtt tgctgatttc aataccaacg gtaaagatca gcatttgcag cattcccatc
    16081 gccgcagaaa tcgtaccttt actcatatcg ctggcaaaca gggttaatcg caccagtccc
    16141 gcattcgcca gaccaatacc gaaagcataa atacttaacc cggcagtcat ccataaatac
    16201 gcgtgcgatg agataaccgt tgccgcagca gcgaccaaca gaccaatcat aatcggccag
    16261 ccgcccataa ttatcagcga acgtacggtg cggcgcgagg tcaaacgcgc taacaacaag
    16321 ttacccgcaa ttaacgcccc gaaaataggc acttgcagca agccatattc atagctgctc
    16381 aactgctcgc cggtaatgat gataatcggc gactgggcga tccacgccag caatggcagg
    16441 ctaacgaatc ccagcgccag cgcccccgcc acaaagcggc cgttcttcag caccagctta
    16501 tagtcacgac cgagttcttt cagtgacagt ttctcgccta tacgcgtggc ggtttcaggc
    16561 atggcttgtt gcagaccgaa aaaggagatc gctgccaatg cggcaaacaa gacaaacatc
    16621 ccttcccagg gcagcacatg gatccacgcc gcgcccacca gcggaccaag tagcggagca
    16681 atcagcgcca cgttcgccat cagcgcggtg atcttgatac aaaccgcctc ttcgaaggat
    16741 tcctgaattg cggcgtatcc cacagcgcca atgaaacaga ggcttatgcc ctgcaagaag
    16801 cgcaacagag tgaattgttc aatgttttgc gccagtaata ttgccagaca ggtgatgata
    16861 aaccacacca cccccgccag catcaccgga cggcgaccaa tacgatccga cagcggcccc
    16921 aggagccatt gtaaaaacat cccgcccgcc agatatgcgg tcattgaagt aggaacccaa
    16981 tcaatgcccg cctgatattg ttccaccacg gccaacatac cgggttgaat catatcgttg
    17041 ccgatatagg ttgaaaattc gtaaagcacc agacagagag ggaaaagtaa cgcctgacgt
    17101 ccaagcctgg caccggaagc taatttattt tgcatgcaat ttcttcgcca ataataatcg
    17161 cgcagagttt aataaaagca cagctaacga gaaagcgaat tttgtagctg aaaccacggt
    17221 taagcacatt cttacattat tgcgagtata gctacgcttt ctttaagttt tatttaacct
    17281 atgcccgtta caattaccca ccgtaaacag gccgcttgag ggaaataaga cgatgccgct
    17341 ttacccagtt taacctgcac tttattctca acgacttgcc tgtattggct cccttttaat
    17401 cactttgcgt cgggaagtta tgctggaaaa tttgaatctc tctctattct ctcttattaa
    17461 cgcgacgcca gactcggctc cgtggatgat ctcgttggcg atttttattg ctaaagattt
    17521 gattaccgtg gtaccgctgc tggccgcggt gctttggttg tgggggctta cagcacaacg
    17581 gcaactggtg ataaaaatcg ctatcgcgct ggcggtcagc ctgtttgtgt cctggacgat
    17641 gggacatctt tttccgcacg accgaccctt tgtcgaaaat atcggctata acttcctgca
    17701 tcatgcggcg gatgactcat tcccaagcga ccacggtacg gtgattttca cctttgcact
    17761 ggcattttta tgctggcatc gcctgtggtc cggctcactt ttaatggtgc tggccgtcgt
    17821 cattgcctgg tcgcgcgttt atcttggcgt gcactggccg ctggatatgc tcggtggatt
    17881 gctggcaggt atgattggct gccttagcgc tcagattatc tggcaagcga tggggcataa
    17941 actctatcaa cgtctgcaat cgtggtatcg cttctgtttt gcattaccga tccgcaaagg
    18001 ctgggtgcgt gactgatctc tgctaaaaag tgtagtattg agcggctcgc ttcaataact
    18061 attcagaggg attatggaaa cacgtcgcga agagcgtatc gggcagctgc tgcaagaatt
    18121 aaaacgcagc gataagttac atcttaaaga cgccgccgcc ctgcttgggg tttcggagat
    18181 gacgattcgt cgcgatctga acaaccacag tgcgcccgtc gttttgctcg gcggctatat
    18241 tgttctggaa ccgcgcagtg ccagccatta cctgttaagc gatcaaaaat cccgcctggt
    18301 ggaagaaaaa cgccgggcgg caaaaatggc tgcgacactg gtagaacccg atcagactct
    18361 cttttttgac tgtggcacca ccacgccgtg gattattgaa gcgattgata atgaaatccc
    18421 ttttaccgcc gtttgttatt cgctaaatac ctttctggcg ctgaaagaga aaccccattg
    18481 ccgcgcattt ctgtgcggtg gtgaatttca cgccagcaac gccattttca aacccatcga
    18541 ttttcagcaa acgttgaata atttttgccc ggatatcgct ttttattccg cggcgggcgt
    18601 gcatgtcagt aaaggcgcta cctgttttaa tcttgaagag ttgccggtaa aacactgggc
    18661 catgtcgatg gcgcaaaagc atgtgctggt tgtcgaccac agtaaatttg gcaaggtgcg
    18721 tccggcgcgc atgggtgacc tgaaacgctt tgatattgtg gtgagtgatt gttgcccgga
    18781 agatgagtat gtgaagtacg cgcagacgca ccgtattaag ttgatgtatt aatgacgtat
    18841 aaccggatga cgtttcgcgc catccggtta tcagaagatt aagagaacca gctgccgaac
    18901 cactgatgga atttcatcat cacgaaatcc cacatccgac caaagaatcc gccctcttcc
    18961 acattttcca tcacgatcag cggacgctgc tcaatggatt taccgttaag ctggaaatca
    19021 atggtcccga caacctgacc ttttttcagc ggtgcggtaa gctgcggttc cgttaacgtg
    19081 tagctcgcct tcaggttttt cagctgccca cgcggtatgg tcactgagcc cgcttcacct
    19141 gccccaagat tcacttcgct cttatcacca aaccagacgc gctgagtcac aaaggtggca
    19201 tcaggtttaa ttggcgtcac ggtttcaaag aagcggaaac cccaggtcaa taatttctca
    19261 gactcattaa aacggatacg gtcagttttc gcccccagca ctacggagat taaacgcata
    19321 tcgccctggg tagccgaagc aaccagatta tatcccgcgc ctgccgtggt tcctgtcttc
    19381 atgccatcaa cattcagatt gctgctccac agcagacggt tacggttagg ctgacgaatt
    19441 ttgttgaagg tgaactcttt ctctttatga atggcgtact cttccggcac atcgtggatc
    19501 aatgctttac ccagcaatgc catatcgcgc gcggtactga actgccccgg cgcatccaga
    19561 ccgtgcaccg tctggaaggt agtgttggtc agacccagtt ttttggcata accattcatc
    19621 aaaccaataa atgactcctg gctcccggca acgtaatcgg ccagcgcaat acaggcgtca
    19681 ttaccggact ggataatcac acctttgttc aagtctgcca ccgaaacctg atcgcccggt
    19741 ttgaggaaca ttaccgatga accacgcagt gccggatttc ccgtcgccca ggcatcttta
    19801 ccgaccgtca ccatatcggt gagtttaatc ttatcggcct taagcgcctg cccaaccaca
    19861 tagctggtca tgattttagt caggctcgcg ggatccagtt tctcatccgc gttgccttct
    19921 gccagcactt taccgctggc gtaatccatt aaaatccatg cacgcgcatc cacgctcggc
    19981 gcttcaacgg tttgttccgc cgcgaatgcc gttggggcaa aaaggaataa aaatgcagaa
    20041 cccgctgcaa gaccacgaag gagagaggag tattgcgtca taaatgccac ccgagtatcc
    20101 attcaaaaaa aatacgttat atcgccgtat gataagttcg gtgattaata acgcacacac
    20161 aaacttaaag aaaccgtaag ttggtaaagt ttttaaagtt tatgcaatat cctgccgctt
    20221 cgctgcttct ggtgtcattt ttttgccagc tgttgcttaa aagttaactt tacttcacca
    20281 tcggagtcct gttcagcctg tggctgcctg tcggaaaagg ggtgagtatg attacgctgt
    20341 ggggtcggaa taattcaact aacgtaaaaa aagtattgct gacgctcgaa gaactggaac
    20401 taccttatga gcaaattctc gcgggccgtg agtttgggat aaatcacgat gctgattttc
    20461 tggcgatgaa ccctaacggg ctggtgccgt tgttgcgtga cgacgaaagt gatctcattc
    20521 tttgggaatc aaacgccatt gtccgctatc tggcggcaca gtacgggcaa aaacgcctgt
    20581 ggatcgactc accggcacgt cgtgcggaag cggaaaaatg gatggactgg gcaaaccaga
    20641 cactcagtaa tgctcatcgc gggatcctga tgggattagt cagaacacca cccgaagagc
    20701 gcgatcaggc cgccattgat gccagttgca aagagtgcga cgccctgttt gccctgctcg
    20761 atgcggaact ggcaaaagta aaatggttct ctggcgacga gtttggtgtg ggcgatatcg
    20821 ctatcgcacc gttcatctac aatttgttta acgttggcct gacctggaca ccgcgtccaa
    20881 atctgcaacg ctggtatcag caactcactg aacgccccgc agtgcgcaaa gtggtgatga
    20941 ttcccgttag ctaattgcgt gggctaactt taagtaattc cccactggac tcgtcggtga
    21001 gaacgtataa ataaccgtcg ggtccagagc gaacatcacg aattcgctgc cctctgtccg
    21061 ttaaaatacg gccatcttct gtcactttgt cgccgttgac gctcatcaca atgacatctt
    21121 tatctttcag cgcgcctata aataattttt gctgccactg ggggaattta tcgctgttat
    21181 agaaggccat gccgctcaca gcgggcgaat ctttccagta aaaaacaggt tgctcggtcc
    21241 cggcgacgat ctcccctttc gcttccggta tcttaaagcc tgaatagttg attccccagg
    21301 ttgccagcgg ccagccgtag tttttgcctt tttgcgggat attaatttca tcaccaccgc
    21361 gcgggccatg ttcattcagc cacagtgcat tactccacgg attcatcgcc attccttgcg
    21421 gattacgaat gccataagac cagatctcgg cgcgcgcacc ggattccttt ataaaaggat
    21481 tatcatccgg gatttcgccc tggtcggtca gacgcaccag tttgccctgt aatttatcca
    21541 gatcctgcgc cgtcgggcgc tgattgtttt cgcccagagc aataaaaaga taacctttac
    21601 cgtcgaatac cagccgcccg ccaaaatggt tgccggtaga cagttttggc atctggcgga
    21661 agacggtgcg gaagtcggtc acttttgaga gatcatcact taagcggcca taacccacag
    21721 cagttccggc tttgccatca tcgccaactt cggaataact taaccagatg cggcgagact
    21781 gagcaaaatc aggcgctaaa accacgtcca gcaggccgcc ctgcccgtgc gcccaaacgt
    21841 ccggaactcc ggaaagcggc gcagataatc cttttcctac ttgccagtga cgcaactcgc
    21901 cgccgcgcag agtgattaac ataccgtgat tatcgggtaa aaaggccagt gcccagggat
    21961 ggtcgagttt gtcttgcagt acttcgacat ttaccgttgc aggagccgcc cagagagcgg
    22021 aagaaagaca aataaggggc acaaggaaaa aggattgtcg atgcataacg cctccggatt
    22081 gactctggct taaagcgtag tcagtggagg agataacaaa ttcattttta caaaaactta
    22141 aacatgaggg gggagacgct ttctccccct tagttttcag gccttctcaa gcatggcgtg
    22201 cttctgcagg ctctggatac tcagcgttaa gctcatcaga caattttcaa gcttatcggc
    22261 gttgacggta ataacagtcg ggcaatcatg gtgcccactc atcaaacata ctgcggctgt
    22321 cgctaatgct tcttcagcat gatgaagagc actccactct tcctgatcca gatgaagatt
    22381 caaccgcagc gatttatcgt gcagttcgcg attcagttta aaaaagttat ctcgtagatg
    22441 attgctttcg ctgacggaca tgtatccttt tgccttcctc agttgcacgt atgcggcgag
    22501 gtcaacgcgt gcgtcgatca accggttcag cagatcgatt cgctgtctgt caacgaacat
    22561 acttcgatcc tcctctttcc agccttgttg caattaaata gtatggctat tttttgagca
    22621 aaatgctgtc caggatcaat tcttcacgcc aaattaacaa aatttacatt tccttcgcct
    22681 ccgccagcga atgagattgc gatacaaatt ttatccagaa ctctgtagaa tccctgccct
    22741 gacctttcct gtaactgaat attttttaag ctatgagcaa agtaactccc cagccgaaaa
    22801 tcggctttgt ttcccttggc tgtccgaaaa accttgtcga ttcagagcgt attctcaccg
    22861 aactacgcac tgaaggttat gacgtggtac cgagctatga cgatgcggac atggtgatcg
    22921 tcaacacctg cggctttatt gacagcgcgg tacaagaatc actggaagcc attggtgaag
    22981 cgttgaatga aaacggcaag gtaattgtga ccggttgtct gggggcaaaa gaagatcaga
    23041 tccgcgaagt ccacccgaaa gtgctggaaa tcaccgggcc tcatagctat gagcaggttc
    23101 tggagcacgt tcatcactac gtgccaaaac cgaaacacaa cccattcctg agcctggtgc
    23161 cagaacaagg tgtgaaactg acaccgcgtc attatgccta tctgaaaatt tcagaaggct
    23221 gtaatcaccg ctgcaccttc tgcattattc cgtctatgcg cggcgacctg gtgagccgtc
    23281 cgattggcga agtgttaagt gaagcgaaac gtctggtaga tgcgggcgtt aaagagattc
    23341 tggtgatctc gcaggatact tccgcctatg gcgttgatgt taaacatcgt actggcttcc
    23401 acaacggcga gccggtaaaa accagcatgg tcagcctgtg cgaacagtta tcgaaactgg
    23461 ggatctggac acgtctgcac tacgtttacc cttatccgca tgtggacgac gtcatcccac
    23521 tgatggcaga aggcaaaatc ctgccgtatc tggacattcc gttgcagcac gccagcccgc
    23581 gcattctcaa actgatgaag cgtccgggtt ctgtagatcg ccaactggcg cgcatcaaac
    23641 agtggcgcga aatctgcccg gaactgaccc tacgctcaac ctttattgtc ggcttccctg
    23701 gcgagacaga agaagatttc cagatgctac tcgacttcct gaaagaagcg cgtctggatc
    23761 gcgttggctg ctttaaatac agcccggttg aaggtgcaga cgccaatgcc ctgcctgacc
    23821 aggttccgga agaagtgaaa gaagaacgct ggaaccgttt catgcagctg cagcagcaga
    23881 tttccgccga gcgcctgcaa gagaaagtgg gccgtgaaat tctggtgatt atcgacgaag
    23941 tggacgaaga aggcgcaatt ggtcgcagca tggcagatgc accggaaatc gacggcgcgg
    24001 tttatctcaa tggtgaaacc aacgttaagc cgggtgatat cctgcgtgtg aaagtcgagc
    24061 acgccgatga gtacgatttg tggggtagcc gggtttaatt aattcaggcc gggcaaaatc
    24121 atttgtccgg cctgcgaata atgattactg ccagctaatc tgtgccctgc cgtttttctt
    24181 actcttatac agccgctcat ccgccagcat tttcaagcgt tcgacggtag gacattcatt
    24241 tgaatgtgct acaccgatac tggtacttaa attaatgctt cccacctgga aatgaacgat
    24301 ttcttttaga atcgatagca actcttccag cgttccaccc gtataaatca ccgcaaattc
    24361 gtcaccgccg aagcggtaga ctttaccttt atctaccggg atctgtgatt taaggatttt
    24421 caccactttc atcaacactt catcgcccag caaatgaccg aaggtgtcgt taatgttttt
    24481 gaatttatcg acgtcaaaca ggcacaaata accgcctttt tccggcagcg cttttaaatc
    24541 ttcgtcaaag gattttctgc ccattgcttt ggtcaggtcg tccaggaacg ccgagttata
    24601 catactgtag agttttttga catcggcagc cgaaaagaaa agcccgccat ccagtacgcg
    24661 ttcaaaatgg ttattaatga tggcttcttt ctccacaata ccacgattaa tcactctgcg
    24721 taccagatag aggcaactgg cacccgatat cactgccacc aatgcggcaa agaaaataat
    24781 caatgtacaa acccgcaaat aaacatcttt tttatacgca aatacataca atccggtcgg
    24841 caaataggcg aagtgctcca tcgcttccga aacggtgaag atggcgtttt tagcattata
    24901 ttcatcaccg gtcaccggtt taccgttctt aaacagagag aacatcaggt tctcgctgtc
    24961 catcgccagc gattttgaca gtaaagaaaa acgctccaga ccaagcaaaa atatcagact
    25021 acgattgtga gttaccggat agaggtaata gacgaaggtt ttaccgtcaa aagagataaa
    25081 ccctgtattt ttgacatgtc gatacttcac aacctgcata atatctgcac aataattttt
    25141 ctcgaactcc tttacctgaa tacattcgtc actggaaatc agataaggac cgacatcgta
    25201 caagtaagag ggtgaaaccg agtcagcgta agctgtgcgt aagccaacca acagctgttc
    25261 ctccgtggga tttagcgtat tgataagatc aattgccacg ctacgccgat attcagcaat
    25321 atttgccacc gcaaaagcag tatcaataac ggactgtttc aggctataca cccgttcctt
    25381 taccatttga gtgtagatcc cgcaggcaac tgctgaaatc atgataaaaa tcagcagact
    25441 gactgtaagt acgaacttat tgattctgga catacgtaaa ctactctttt actaattttc
    25501 cacttttatc ccaggcggag aatggcatgg gtttctggta cagatacccc tgagcctgaa
    25561 cacatcctaa tttaattaat tcgcgcgcaa cgtcttcgct ctctacgcct tccgcaatca
    25621 ccgggatccg gttatagcgt gccagatcga aaataatgcg cacaattttt cccgcagtac
    25681 cgaaaatatc gccggtaaat tgcgagtcca cctttatgct gtctggatta atttcataaa
    25741 aacgtttcag gtttgacaaa ccggtgccaa agtcatcaat cgatatccgt accccttgtt
    25801 tacgcagttg ttcaatattg agataaaaat gttcattaat tcgctcgatc acggtttcag
    25861 tgatctcaac acagaggctg ttcgccttca ggccattttc tttcatgatg gtggtaaaac
    25921 gctcatgaaa gtgcggttcg tttaactgcc agggcgaaac attaatatgc agtaagaaat
    25981 catccttcag cgacgcacgc tgactaaaat gtctgaattc acccattgcc gttttaataa
    26041 tctgataacc cagctcattg ataaacccga tatcttcagc aatggtaatg aacttcagcg
    26101 gtgaaatgat cccccgctcc gcagaaaccc aacggcacaa cgcctccgcg cccagaatgg
    26161 cgcgggtgtt aatatcaacg atcggttgat agacgaggaa tagttcgcca atttccattg
    26221 catggcgaat attacgcgca atccagatat tctcttctat ttcgttgagt ttcgcgtcat
    26281 cacaaaaaat aaactcggag aacatatggt cacggatact ggaaatcgcc agtttcagtt
    26341 tttctgcgta actccaggcg ttttccccct gcaaaggttc acaaatggca gacttatgga
    26401 tgcgcagatt gcggtagcca aagctgctgt caaaatcgtt taccatcgct attttacggg
    26461 taaatgtctg tacattttcc cgacacagaa gtaaatagag atcggcattg aatgtcacga
    26521 tatcgcagca ttgcgcatat ttctcggcaa ttcgctgaca caatttcctg acgattttat
    26581 tgcgttcatc aacaccaaac accgcttcca cggcttccag accacacagt ttgatgatcc
    26641 ccacagccag atactgatta ttgttggact caataatcgc catattttta ataaaaccgc
    26701 tttcctgatc gtaagagatc agtgagagca atttatttga ccgttcaatt tcggcatctt
    26761 tcatttccgc gatcgaattt ttgatgctga taatctcttt cgcatcgcct tcgcgaattt
    26821 tcagagcctc gatctgatcc gttgagtcgg gcaaaaacgc cagcgtatta accagctcgg
    26881 tcatttcaga aaattgccga cggaacacac gagtggtcat gaaatatacc accagcaaca
    26941 cgataataat gaacgcaaac ggtagataaa aaagtttatc tgatgcgttc aataaattac
    27001 cgccaggata ataaaaatag atttcgaaca atgcattatt tggcactgct gatgtctttg
    27061 caatatactc cgtatcgaca gaatcaataa tattataatt acctccgcta ttataaataa
    27121 tcagtttttc acgagcgagt tgattcttgg caatgatctc atgaccttgt ttcaaaaata
    27181 gaacttcatc attagcgctg atttgatcaa gatactcgtt cagataccgc cagtttaaat
    27241 cgacatgaat tttatctacc gctaacaaat cgtgatcttt gccgttgata cgtagcgtaa
    27301 aagagataca ttttttaccc ggacgatcaa agcaattgta tgccggattc cagctgttgt
    27361 tagagtcatc tgaagccacc gaccacggtc gtttcgatgg gttaaaatcg gcaaaaataa
    27421 cccgccctga ctcgtcgaca agcggtccat ttacttgttc ctgatgataa acaacgattt
    27481 tatcattcgc cctccgcgaa aagagataat atttattatc atcaagtacc agtcccaccg
    27541 agtctaaaaa tggcatcatt tccattttca gaatcacggc gcgttgaatc tcctctgtat
    27601 tttttaataa atacagtgag tcctttaatg tcagggccga acgttcaata tctttaaaga
    27661 aatagccaac gtttttctga ataagttcaa agcggctatc ggcacgtttt accgcgcgtt
    27721 ccgataccag ttgatagtta atgatgaaaa aaagaccaat aaatgacagt gctgccaata
    27781 aaaataaaat tatcagcctt atctttatct tttcgtataa actcagcatg ttaatacatt
    27841 taacaaaata actatctgaa cgctgtgccg ctgcaaaaat agtcccttgc tgcggctttt
    27901 taaccaaatt acccgattct acaaaatcag cagaaagtta caacacttgc ttagggcaat
    27961 aattgcctgt gaggtgatta ccctttcaag caatattcaa acgtaactat cctttaattt
    28021 tcggatccag cgcatcgcgt aaaccatcgc ccaacaaatt gaacgccagt acggtcagaa
    28081 aaatagccag ggccggaaaa acagcgacat gcggcgcgat aaccatatcc gctcgtgcct
    28141 cattgagcat tgctccccac tctggtgtcg gcggctgcgc accgaggccg agaaatgaca
    28201 ggctggcggc agagataatc gaggtgccaa tgcgcatggt gaaaaacacc gcgatagacg
    28261 agacggtccc aggcaagata tgacgcaata aaatggtcat atcgctggca ccaatactgc
    28321 gtgctgactc aataaaggtt tgctgtttca acaccagcgt gttgccgcgc accaggcggg
    28381 caaacgcggg gatggaaaaa atggcgactg cgataatcac gttagcaatg ccgcttccca
    28441 acaccgcaac aacagcgatc gccagtaaaa tacccgggaa ggcaaacagc acatcgcaaa
    28501 tgcgcatgat cagccgatcc caccagcctt cataatatcc agccagcaag cccagcaacg
    28561 tccctatcgc cgcaccgata aacacggcaa acacgcccgc cgccagcgag atttgcgcac
    28621 caaccaggac acggctgaaa atgtcgcgcc ccagtgaatc gacgccaaac cagtgctgca
    28681 aagaaggtcc gttattcaga ttgtcataat caaaataatt ttcggcgtca tagggagcga
    28741 tccagcgtgc aaaaatggcc accacaatca ataaaataac gaataatgcg gcggtcatcg
    28801 ccatatgctg acggcgaaat cgtcgccaga attcatgcca cggtgtacgc acctggtcag
    28861 gtttgaccag tggcatggcg tttaacaccg cctgacgtcg ccagttaaat agtcgcatcc
    28921 ttacttgtac ctgatagccg ggttaatggc ggcgtaaagc acatccacca ctaagttgat
    28981 aagaataaat tccagcgaga aaagcagaat ttccgcctga atcaccgggt aatcgcgcat
    29041 ttctacggag tcaacgagta agcgtccaag tccgggccag ttgaaaactt tctccaccac
    29101 gatagaacca ccgagcaaaa agccaaactg taagcccatc atggtcacta ccgggatcat
    29161 cgcgttacgt agcccgtgtt tgaggacaac ccaggtttcg ctcacccctt tcgccctcgc
    29221 ggtacgcata taatcttcgc ttaaaacatc gacaaacgac gcgcgggtaa agcgcgccat
    29281 cacggctgcc actgccgcgc cgagcgtcag ggagggtaaa atgtagtgct gccaactgtc
    29341 tgctcccacg gtaggcagcc agcccagttc aacggagaat acctgaatta aaagcatccc
    29401 cagcgcaaat gccggaaacg agatccccga caccgcaatg gtcatactca atcgatccgg
    29461 ccaacgatta cgccagacgg cggcgataat tcccgccgcc ataccaaata tcaccgccca
    29521 gaccatactg gttatggtca gccacagcgt tggcataaag cggctggcaa tctcatcggc
    29581 aaccggacga cgcgacacca tcgacaggcc aaaatccccc tgcacagcat tgctgatata
    29641 gtgccagaac tggtgataca gcggctgatc caaccccagc tgctgacgca ccagttctat
    29701 aacctgcgca tcagcttcgg gcccggcaat caatcgcgcc ggatcgccgg gcagcatatg
    29761 cacaaataaa aacaccagca ccgagacgat aaacagcgtc ggaatcaacc ccagtaagcg
    29821 tttgataacg taattaagca ttccactccc tgcgttgctt attgcaaatc cgcgtcttca
    29881 aagctgaagc cggtgtctgg catgatccaa aaaccggtca ggtttttact gtgtgccgac
    29941 accagttttt ctaccaccag cgggatccac ggcgactctt gccagatgat atcctgcgcc
    30001 gccttataca agcgggtctt ttccgccgga tcattagttt tcagtgcctg agccaggaag
    30061 tcgtccacct gtttattgct gtaaaatgcg gtattaaaca gcgtcggtgg ccagttttgc
    30121 gaggcgaaca gtggcgatag cgcccagtcc gcttcgccgg ttgaagccga ccagccagtg
    30181 tagaacatcc gcacgccgct ctctttttgc cctttacctt caacttctgc cgcccgctgt
    30241 ccggcatcca tcgcagtcac ctggacttta atcccgacct gcgctaactg ctgctgggta
    30301 aattgcagca ctttctgcgc ggtgctgtgg ttatgtgacg accacagcgt ggtactgaaa
    30361 ccgttgggat atcccgcctc tttcagtaat tcgcgcgctt tcactggatc gtaaggccac
    30421 ggtttataac tttgcgcgta ggcgatactt ggcggtacca caccagtagc tggcgttgca
    30481 tagcccgcaa aggcaacttt caccagcgcc ggacggttaa tggcgtaatt cagcgcctca
    30541 cggaccttcg ggttatcgaa cggcttttgc gtcacgttca tactgatata acgctgcata
    30601 attgacggac tggccatcaa ctcgatattt ttgtttttct ccagcagtgt ggcctgctcg
    30661 tagggaatgg ggaaagcaaa ctgcgcttca ccggtttgca gcattgccgc gcgggtgttg
    30721 ttatccgcca ccggacgcca ggttatgctg tccagtttgg gcaatcctgg ctgccagtaa
    30781 cccgcgaatt ttttcacctt cacaaaatcg gtctgattcc aggtatccag ttcatacggt
    30841 ccggttccca ccggatgaaa accaatctcc ttgccatatt tttccagcgc tgccggtgaa
    30901 atcatcgcgg tcgccggatg ggcaagaata ttaataaacg ctgagaacgg ctgtttgagg
    30961 gtaatcttta ctgttgttgg atcgatcgcc tccgttttgg caatattctt atacaggtta
    31021 tagcgtttaa gatgattcgc cggatcgctg gcccggtcca gattcgcttt caccgccgcg
    31081 gcgttgaaat cggtgccatc ctggaattta atgccttccc gcaatttcac ggtgtaagta
    31141 atgccgtcat cggaaacggt ataactctcc gccagcacgt ttttcagttt catctcttta
    31201 tccagaccga acagcccctg gtaaaacgat ttcgctacgg cctgagataa cgtgtcattc
    31261 gcgtcatacg gatcgagcgt ggtgaaattc gatcctaccg ccaccaccac atctttggca
    31321 gcgaatgcac aagatgccat caacgctgtc gcaatgccca gcgccactaa cccactacgg
    31381 tgtacagctc ttgccatttt attctccgcc tgaatgttat ctacgcatga atgcgtattc
    31441 tgattgtggt tgtgcgacgt aatgccccgg cccgacgcat tgcaacgaga cggctgccac
    31501 ctcttcgcca cgcagatgaa tattgctggg aagatcgtcc gacagcagta cacgctgcgg
    31561 tcgttgtcgg gacggttcag cgaccggaac tgccgccagt aatttacgcg tataaggatg
    31621 ctgcgggttt tcgaagaccg cgcgccgtgg accaatttca acaatttgcc cgagatacat
    31681 caccgccaca cgatgactaa tccgctctac cacagccata tcgtgggaga taaacagata
    31741 cgcaatgccg aaatcacgct ggagatcgag caacaagttg ataatctgcc cgcgaataga
    31801 aacatccagc gccgaaacgg cttcgtcggc aatgatcact tttggattca atgccaacgc
    31861 gcgagcaatg cagatgcgct ggcgctgacc gccggaaaac tcatgcgggt aacgccaggc
    31921 atgttcaggt aacaggccca cgcgctccag caaccacgca acgcgtgcag ccgcttcttt
    31981 acctggcaat aaaccgtgta cacgcagcgg ttcgataatc gaatcaccga tggtctgacg
    32041 tgggtccagc gaagcgtaag ggtcctgaaa aataaactga atatcccggc gcaatgcctg
    32101 aagtttgccg ggtgacaagg tatcgattcg ctgaccgtta aagataattt cgccgccctg
    32161 cgattcgacc aggcgcagca acgcccgccc ggtagtggat ttaccgctgc cagactcgcc
    32221 caccagcgat agcgtttcgc caggccagag atcaaaactg actttctcaa cggcatgcac
    32281 ttcccgcgtt acgcgattca acaaaccgct gcgcaaaggg aaacgggtga ccagattacg
    32341 cacccgtaaa acaggttcac catccaccac cgttttctgc tcgatggggg ggcctgtttc
    32401 gctggatgtt caagcgatat caatgggaaa cgtcggggat aatctaaccc tttcatcgca
    32461 ccaagttgcg gaacagcagc taacagcgca cgggtgtaag gatgttgcgg tgcatgaaaa
    32521 atctgttcga cggtacccgt ttccaccgcc tcgccctgat acatcaccag tacccgatcg
    32581 gcaatctctg ccaccacgcc catatcgtga gtgataaaga taacgcccat cgacatctct
    32641 ttttgcaata ctttgattaa ttgcaggatc tgcgcctgaa tagtgacatc cagcgcggtg
    32701 gttggctcat cggcaatcag caccgccggg cggcatgaca gcgccatcgc aatcatcact
    32761 cgctggcgca tcccgccaga gagttgatgc ggataacgtg aaagaatggt ttgtgcctca
    32821 ggaatgcgta cctgatccag catccgcttc gcctcgacca tcgcttcttc acgactggcg
    32881 ttctgatgca gacgaattga ttcggcaatc tgttcaccca cagtaaatac cgggttcagc
    32941 gatgtcatcg gctcctgaaa tatcatcgcc atatccgcac cgcgaacatg gcgcatttgt
    33001 gcagcgctct gctcgctaag ttcaatcact tcgcgactgc gccgctgcaa cagcatttta
    33061 tcgcactgta ctaaaccgcc cgcctgttcc aacaggcgca tcaatgccaa cgcagtcact
    33121 gacttaccgg agccggattc gccaacaatt gccagcgtct caccgcgttg cagactaaaa
    33181 gagagattgc ggaccgcagc tattttctgc tggtcctgca taaaggcaat attcagattt
    33241 tcaaccgcca gcacattacc ggcatcaagt tcatcactgt gtggcaacgg tgtccccttt
    33301 ttcacggtag ataccggtgg ttggcgtatc gcctgcgtag ccccaggcgc gatacattcc
    33361 ttcggtgtta aacggtagcg cgacattccc ttcatggtcg atagcgatta agccaccgct
    33421 accgccaagc gcagggagtt tttccattac tacccgctcg caggcttccg cgagacttaa
    33481 tccgccgtaa tccattaacg cggcgatgtc atatgccgcc agcgcgcgga tgaagacttc
    33541 gcccgtgccg gtacaagaaa ccgccacact ggcgttattg gcgtagcatc cggcacccac
    33601 taagggacta tcgccaactc gtccgggtaa tttattggtc attccgcctg tggacgtggc
    33661 tgccgccaaa ttgccgtcta aatccaacgc cacggccccc acggtgccca ttttctgttt
    33721 ttcatccagt ggcgcaccgc tatggtcgag gacggttgcc ccttccttgc gcgctgccag
    33781 tagttgttca taacgcaaag gcgtggagaa aatctccggc gagacgcgct ccatgccacg
    33841 agcaaacgca aaattttctg ccccttcgcc aatcatcatc acatgcgggc tttgctccat
    33901 caccagccgg gcggcaagaa ccggattacg cagatgacta acgcccgcca ccgcaccggc
    33961 tttcagggtg ttaccatcca tcacacaggc gtccagttca tgggtttcat cacgcgtaaa
    34021 gacagcgcca attccggcgt taaacagtgg acactcttcc agcagacgca ccgcttccgt
    34081 caccacatcc agcgcacttt cgcccgcttc cagcattttc tgcccggttt caacaatggc
    34141 agacaacgcc tcaatgtagc gtaattcctg ttgcagactc atctgcgcgc ggctaattgc
    34201 acctgcgcca ccatgaattg caatgactgc tttgcccata gtattcgtcc attaagagtg
    34261 gctcgccact tttgctataa atatcagaat ttttatgaat tactcattca atttttgaat
    34321 atagaaagat ggatatggca tgtaaaggca ggagccaacg ctacgcccga ggtggcatac
    34381 agtctgtggc gtttttctga cataataggc aaattcgatt ttgcctccgc aggagtgttt
    34441 tcatggaatt taccaccgga ttgatgtcgc tcgacaccgc gcttaatgag atgctttctc
    34501 gcgtcacccc actgaccgcc caggaaacgc tgccactggt acagtgtttt ggtcgtattc
    34561 tggcgagcga tgtcgtttcg ccgcttgatg ttccggggtt tgataactcc gcaatggacg
    34621 gctacgcggt gcgtttagcc gatattgcct ccaggcaacc gctgcccgtt gccggtaaat
    34681 cctttgccgg tcagccgtac catggtgaat ggcctgcggg tacctgcatt cgtattatga
    34741 ccggtgcgcc ggtgccggaa ggctgcgaag cggtggtgat gcaggagcag actgaacaaa
    34801 cggacaatgg cgtgcgtttt actgctgaag tgcgtagcgg gcaaaatatt cgccgtcgcg
    34861 gtgaagatat ctctgcaggt gcggttgttt tcccggcggg aactcgcctg actaccgctg
    34921 aactgccagt gattgcttca ctggggattg ccgaagttcc ggtgattcgt aaagtgcgtg
    34981 tagcgctttt ttctaccggt gatgaactcc agttgcccgg tcagccactg ggcgatggcc
    35041 aaatctacga taccaaccgt ctcgccgtac acctgatgtt agaacagttg ggatgcgagg
    35101 taatcaactt agggattatc cgcgacgatc cacatgccct gcgcgccgca tttattgaag
    35161 ccgacagcca ggcggatgtg gtgatcagtt ccggcggtgt ttcagtgggt gaagcggatt
    35221 acaccaaaac gattcttgaa gagctggggg agatcgcctt ctggaagctg gcgattaaac
    35281 caggtaaacc gttcgcgttc ggtaaactca gcaatagctg gttctgcggc ctgccgggca
    35341 acccggtttc agcgacgctg accttctatc aactggtaca gcctttgctg gcaaaactaa
    35401 gcggcaacac cgccagcggc ctgcccgcgc gccagcgcgt acgcacagcg tcccgcctga
    35461 aaaaaacgcc aggacgtctt gatttccagc gcggcgtgct gcaacgcaac gccgacggtg
    35521 aacttgaagt gacgaccacc ggacatcagg gttcacatat atttagctcc tttagtctcg
    35581 gcaactgctt tatcgtgctg gaacgcgatc gcggcaatgt ggacgtgggc gaatgggtgg
    35641 aagtagaacc gtttaacgcg ttgttcggag gcctgtaatg gcggaactca gcgatcagga
    35701 gatgctgcgc tacaaccggc aaatcattct gcgcggtttt gattttgacg gtcaggaggc
    35761 gctaaaagat tctcgtgtgc tggtagtcgg cctcggtgga ctcggctgcg cagcttcgca
    35821 gtatctggca agcgccggtg tcggcaacct gacgctgctc gacttcgaca cggtttcgct
    35881 ctcgaatctg caacgccaga cgctgcacag tgatgctacg gtcgggcaac cgaaggtaga
    35941 atccgcccgt gacgccatga cgcggatcaa cccacatatc gcgattacgc cagtcaatgc
    36001 actgcttgat gacgctgaac ttgctgcgat gattgcaaaa cacgatctgg tactcgactg
    36061 tacggataac gttgcggtgc gtaatcaact gaacgctggc tgttttgccg cgaaggtgcc
    36121 gctggtttcc ggcgcggcaa ttcgtatgga agggcaaatc acggtcttca cttatcagga
    36181 cggcgaaccg tgctatcgct gccttagccg tttgtttggc gagaacgcat taacctgcgt
    36241 agaagcaggc gtgatggcac cgttgatcgg cgtaattggt tcgttgcagg caatggaagc
    36301 gatcaaactg ctggcaggtt atggcaaacc ggccagcggg aaaatcgtca tgtacgatgc
    36361 gatgacctgt cagtttcgcg aaatgaaact gatgcgcaat ccggggtgtg aggtgtgcgg
    36421 gcagtaattg ccacctccag gccagataag acgcggcact ccgccacgtc atcagcaatt
    36481 tatgaggatg cagaacgtcc ggttaaatcg acgttctgcc aaacgctccc agccagtcct
    36541 gttcaaactt cgccacagcg gcttccaccg ccggatagct aatcatctgt tgtgccacat
    36601 ccagtggcag cgtaattgat tcacatcctg ccagtaagca gtccagcgcc tgacgcgggg
    36661 ttttgaaact cgctgccagc actttcgcct gcggcgcatg cattttcaat aactggtgta
    36721 agtcggtcac agtctgaatg ccgctaccgc cctgagcatc aatacgatta acgtaaggcg
    36781 caacatattc cgcacctgcc agcgccgaca gcagcccttg tgctgcgcca tataccgcgg
    36841 ttcccagcgt tggaatccct tccgctttta acatcttaat agctgccagc ccctcggcgg
    36901 tcaccggaac tttcaccacg atatccgcaa taatagaacg caatttacgt gcgtcattca
    36961 ccatcccttc agcagtggta gccattacct gggcaaacag acgcccctga ccgcccatcg
    37021 cttcatgaag ttgcggaagc acaacatcca gcggtttttt acccgcggcg ataatgcttg
    37081 ggttagtggt cacacccgcc agcggaaaaa tacgtgacag cgccttcacc gcaacaacgt
    37141 ctgaagtatc cagatacagt tccataacca tcctcaaaat gtactttttg tctgaatgta
    37201 gactatcacg ggaaaactct ctgaacagtt gacgcacatc aatctaactt tcattcgaaa
    37261 gtattttaat gtttgaacga aacagagagg caactatgat tttcaatatt cagcgctact
    37321 cgacccatga tggccccggt atccgcacgg tcgtatttct taaaggctgt tcgctgggct
    37381 gccgctggtg tcagaacccg gaaagccgcg cccgcacgca ggatctgctg tatgacgcac
    37441 gactgtgtct ggaaggctgc gagctgtgcg ctaaggccgc gccggaagtg attgagcgcg
    37501 cgctgaatgg tttgcttatt catcgggaaa agttaacccc ggagcatctg acggcgttaa
    37561 ccgactgctg tccgacacag gcattaaccg tgtgtggtga agtgaaaagc gttgaggaga
    37621 tcatgacgac cgttctgcgc gataaaccgt tttacgatcg cagcggcggc ggtttaacgc
    37681 tttcgggtgg tgagcccttt atgcagccgg aaatggcgat ggcgctactg caagccagcc
    37741 acgaggcagg cattcatact gcggtagaaa cctgtctgca tgtgccgtgg aaatatatcg
    37801 ccccttctct gccctatatc gatctgtttc ttgccgattt aaaacacgtt gccgacgcgc
    37861 cgtttaaaca gtggaccgac ggtaacgccg ccagagtact ggataacctg aaaaaactcg
    37921 ccgcagcggg caaaaaaatc attatccgcg tgccgctgat tcagggcttt aatgccgacg
    37981 aaacctctgt aaaagccatt accgattttg ccgccgacga gctgcacgtt ggcgaaattc
    38041 attttctgcc ctaccacacg ctgggcatca acaaatatca cttacttaat ctgccctatg
    38101 acgccccgga aaaaccgctt gatgcgccag aactgctcga ctttgcccag cagtatgcct
    38161 gccagaaagg gttaaccgcg accttacgag gataacaacc atgaccacac tgaaactgga
    38221 cacgctcagc gaccgcatta aagcgcacaa aaatgcgctg gtgcatattg tgaaaccgcc
    38281 agtctgtacc gagcgcgcgc agcactatac cgagatgtat caacaacatc tcgataagcc
    38341 gatcccggta cgtcgcgcgc tggcactggc gcatcacctg gcgaatcgca ccatctggat
    38401 caaacacgat gagttgatca ttggcaacca ggcaagcgaa gttcgcgccg cgccgatctt
    38461 cccggaatat actgtctcgt ggatcgaaaa agagattgat gatctggcag atcgtcccgg
    38521 tgctggcttt gcggtgagcg aagagaacaa acgcgttctg catgaagtgt gcccgtggtg
    38581 gcgcggtcag accgtacagg atcgctgcta cggcatgttt accgatgagc aaaaaggtct
    38641 gctggcgacc ggaatcatta aagcggaagg caatatgacc tccggcgatg cgcacctggc
    38701 ggtgaatttc ccgctgctgc tggaaaaagg tcttgatggt ctgcgtgaga aagtagcgga
    38761 acgtcgctcg cgcatcaatc tgacggtgct ggaagattta catggcgaac aattcctgaa
    38821 agcgattgat atcgtgctgg tggcagtcag tgaacacatt gaacgtttcg ctgccctggc
    38881 gcgtgaaatg gctgctaccg aaactcgcga aagccgtcgc gatgaactgc tgacgatggc
    38941 agaaaactgc gatcttatcg cccaccagcc gccgcagact ttctggcagg cgctgcaact
    39001 gtgttacttc atccaattga ttttgcagat cgaatctaac ggtcactcgg tatcgtttgg
    39061 tcgtatggac cagtatctct acccgtacta tcgccgcgac gttgaactca accagacgct
    39121 ggatcgcgaa cacgccatcg agatgctaca tagctgctgg ctgaaactgc tggaagtgaa
    39181 caagatccgc tccggctcac actcaaaagc ctctgcggga agtccgctgt atcagaacgt
    39241 taccattggc ggacaaaatc tggttgatgg tcaaccaatg gatgcagtga atcccctctc
    39301 ttacgcgatc ctcgaatcct gcggtcgcct gcgttccact cagcctaacc tcagcgtgcg
    39361 ttaccatgca ggaatgagta atgatttcct cgacgcctgc gtacaggtga tccgttgcgg
    39421 cttcgggatg ccggcgttca acaacgacga aatcgtgatc ccggaattta ttaaactcgg
    39481 tattgaaccg caggacgctt acgactacgc agcgattggt tgtatcgaaa ccgccgtcgg
    39541 tggcaaatgg ggctatcgct gtaccggcat gagctttatc aacttcgccc gcgtgatgct
    39601 ggcggcgctg gaaggcgggc gtgatgccac cagcggcaaa gtgttcctgc cacaagaaaa
    39661 agcgttgtcg gcaggcaact ttaacaattt cgatgaagta atggacgcgt gggatacgca
    39721 aatccgttat tacacgcgta aatcaatcga aatcgaatat gtcgtcgaca ccatgctgga
    39781 agagaacgtg cacgatattc tctgctcggc gctggtggat gactgtattg agcgagcgaa
    39841 aagtatcaag caaggcggcg cgaaatatga ctgggtttct ggcctgcagg ttggcattgc
    39901 caacctcggc aacagcctgg cggcagtgaa gaaactggta tttgaacagg gcgcgattgg
    39961 tcagcaacag ctggctgccg cactggcgga tgacttcgat ggcctgactc atgagcaact
    40021 gcgtcagcgg ctgattaacg gtgcgccgaa gtacggcaac gacgatgata ctgtcgatac
    40081 gctgctggct cgcgcttatc agacctatat cgacgaactg aaacagtacc ataatccgcg
    40141 ctacggtcgt ggtccggttg gcggcaacta ttacgcgggt acgtcatcta tctccgctaa
    40201 cgtaccgttt ggcgcgcaaa ctatggcaac gccggacggg cgtaaagccc acaccccgct
    40261 ggcagaaggc gctagtccgg cctctggtac tgaccatctc ggccctactg cggtcattgg
    40321 ctcggtgggt aaactgccta cggcagcgat tctcggcggc gtgttgctca accagaaact
    40381 gaatccggca acgctggaga acgaatctga caagcagaag ctgatgatcc tgctgcgcac
    40441 cttcttcgag gtgcataaag gctggcatat tcagtacaac atcgtttccc gcgaaacgct
    40501 gctggaggcg aaaaaacatc cggatcagta ccgcgatctg gtagtgcgtg tcgcgggtta
    40561 ttccgccttc ttcaccgcgc tctctccaga cgctcaggac gatatcatcg ctcgtactga
    40621 acatatgctg taatctcttc ggcccggctg cggcgtcgcc gggctaaatc attcacatca
    40681 tcaatttcat ccttactttc attcgaaata taatttgtgc tctgcgtcac attgttatta
    40741 cactgttacg gtttcatttt cgagccagga gcacacctat gagcgtaaaa gttatcgtca
    40801 cagacatgga cggtactttt cttaacgacg ccaaaacgta caaccaacca cgttttatgg
    40861 cgcaatatca ggaactgaaa aagcgcggca ttgagttcgt tgtcgccagc ggtaatcagt
    40921 attaccagct tatttcgttc tttcctgagc taaaggatga gatctctttt gtcgcggaaa
    40981 acggcgcact ggtttacgaa catggcaagc aactgttcca cggcgaactg acccgacatg
    41041 aatcgcggat tgttattggc gagttgctaa aagataagca actcaatttt gtcgcctgcg
    41101 gtctgcaaag tgcatatgtc agcgaaaatg cccccgaagc atttgtcgca ctgatggcaa
    41161 aacactacca tcgcctgaaa gctgtaaaag attatcagga gattgacgac gtactgttca
    41221 agttttcgct caacctgcca gatgaacaaa tcccgttagt gatcgacaaa ctgcacatag
    41281 cgctcgatgg cattatgaaa cccgtcacca gtggttttgg ctttatcgac ctgattattc
    41341 ccggtctaca taaagcaaac ggtatttcgc ggttactgaa acgctgggat ctgtcaccgc
    41401 aaaatgtggt agcgattggc gacagcggta acgatgcgga gatgctgaaa atggcgcgtt
    41461 attcctttgc gatgggcaat gctgcggaaa acattaaaca aatcgcccgt tacgctaccg
    41521 atgataataa tcatgaaggc gcgctgaatg tgattcaggc ggtgctggat aacacatccc
    41581 cttttaacag ctgacctctc taccggagcc gtgcgctccg gttttccctt ctttttacat
    41641 cctgtacatc acatcaaatc attaaacttg cattaactta tttataactt atattgtcac
    41701 atgcaaatca gaatggtttc gaatgaaagt taatgagagg tggttatggc ttctactttt
    41761 accagcgaca cattgcctgc cgatcacaaa gcagctatcc gtcagatgaa gcacgcgctg
    41821 cgggcgcagc ttggcgacgt ccagcagatc tttaatcagc taagcgatga cattgccacg
    41881 cgagtggctg aaatcaacgc actcaaagca cagggcgatg ccgtctggcc ggtgctgtct
    41941 tatgccgata tcaaagcagg tcatgttact gcagagcagc gcgaacagat taaacgtcgc
    42001 ggttgtgcgg tgataaaagg ccatttcccc cgcgaacaag cgctaggctg ggatcagtcg
    42061 atgctggact atctggaccg caaccgcttt gacgaggtct acaaaggccc cggcgataat
    42121 ttcttcggga cgctcagcgc ttcacgtccc gagatttacc ccatctactg gtcgcaggcg
    42181 caaatgcagg cccgccagag tgaagaaatg gcgaatgcgc agtcgtttct caatcgtctg
    42241 tggacatttg aaagtgatgg aaagcaatgg tttaacccgg atgtgagcgt catctaccct
    42301 gaccgtatcc gccgccgtcc gcccggaacg acctccaaag gtcttggagc gcataccgac
    42361 tccggggcgc tggaacgctg gctgcttcca gcgtatcagc gcgttttcgc caacgtcttt
    42421 aatggcaatc tggcgcaata tgatccctgg catgcggcac atcgtacgga agttgaagag
    42481 tacacggtgg acaacaccac caaatgttcc gtgtttcgga cattccaggg ctggacagcg
    42541 ctctctgata tgctgcctgg tcaggggctg ctgcacgttg tgcccattcc tgaagccatg
    42601 gcgtacgtac tgttacgtcc gctgcttgat gatgtgccgg aggatgaact gtgcggcgta
    42661 gcgcccggaa gagtgttgcc ggtatcagag caatggcatc cactgttaat tgaggcgtta
    42721 accagcattc caaaactcga ggccggagac tccgtctggt ggcactgcga cgtcatccat
    42781 tccgttgccc ccgttgaaaa tcaacagggt tggggcaacg tgatgtacat tcctgcggca
    42841 ccgatgtgcg agaaaaatct tgcctacgcg cacaaggtga aggccgcact ggaaaaaggc
    42901 gcatcgccgg gcgacttccc gcgcgaggac tatgaaacaa actgggaagg acgctttacg
    42961 cttgccgacc tcaacattca cggtaagcga gcgttgggca tggatgtttg agagttaatg
    43021 gaagtgaatt gatgtgggat taagaaaatc gattcgacgt tactcagatt gctgacaagt
    43081 gcgctttctt aatgcctgat gcgctacgct tatctggcct acaaagtcct gcagattcaa
    43141 tatattacag gtac
//
LOCUS       DAZGSP010000036        43100 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZGSP010000036 DAZGSP010000000
VERSION     DAZGSP010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.036"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(219..1076)
                     /gene="murI"
                     /locus_tag="ACHK74_002094"
     CDS             complement(219..1076)
                     /gene="murI"
                     /locus_tag="ACHK74_002094"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFP0220916.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(1021..2865)
                     /gene="btuB"
                     /locus_tag="ACHK74_002095"
     CDS             complement(1021..2865)
                     /gene="btuB"
                     /locus_tag="ACHK74_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFP0220917.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQLPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHFGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2930..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3234..4334
                     /gene="trmA"
                     /locus_tag="ACHK74_002096"
     CDS             3234..4334
                     /gene="trmA"
                     /locus_tag="ACHK74_002096"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFP0220918.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4374..4733)
                     /gene="yijD"
                     /locus_tag="ACHK74_002097"
     CDS             complement(4374..4733)
                     /gene="yijD"
                     /locus_tag="ACHK74_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFP0220919.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4733..5383)
                     /gene="fabR"
                     /locus_tag="ACHK74_002098"
     CDS             complement(4733..5383)
                     /gene="fabR"
                     /locus_tag="ACHK74_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFP0220920.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDIGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5714..7114
                     /gene="sthA"
                     /locus_tag="ACHK74_002099"
     CDS             5714..7114
                     /gene="sthA"
                     /locus_tag="ACHK74_002099"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFP0220921.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(7097..8014)
                     /gene="oxyR"
                     /locus_tag="ACHK74_002100"
     CDS             complement(7097..8014)
                     /gene="oxyR"
                     /locus_tag="ACHK74_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFP0220922.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(8266..9549)
                     /locus_tag="ACHK74_002101"
     CDS             complement(8266..9549)
                     /locus_tag="ACHK74_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0220923.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            complement(9616..10830)
                     /gene="manD"
                     /locus_tag="ACHK74_002102"
     CDS             complement(9616..10830)
                     /gene="manD"
                     /locus_tag="ACHK74_002102"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFP0220924.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            complement(11328..12701)
                     /gene="argH"
                     /locus_tag="ACHK74_002103"
     CDS             complement(11328..12701)
                     /gene="argH"
                     /locus_tag="ACHK74_002103"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFP0220925.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(12762..13538)
                     /gene="argB"
                     /locus_tag="ACHK74_002104"
     CDS             complement(12762..13538)
                     /gene="argB"
                     /locus_tag="ACHK74_002104"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFP0220926.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(13546..14550)
                     /gene="argC"
                     /locus_tag="ACHK74_002105"
     CDS             complement(13546..14550)
                     /gene="argC"
                     /locus_tag="ACHK74_002105"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFP0220927.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            14704..15855
                     /gene="argE"
                     /locus_tag="ACHK74_002106"
     CDS             14704..15855
                     /gene="argE"
                     /locus_tag="ACHK74_002106"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFP0220928.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            16207..18858
                     /gene="ppc"
                     /locus_tag="ACHK74_002107"
     CDS             16207..18858
                     /gene="ppc"
                     /locus_tag="ACHK74_002107"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFP0220929.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            19041..20774
                     /gene="eptC"
                     /locus_tag="ACHK74_002108"
     CDS             19041..20774
                     /gene="eptC"
                     /locus_tag="ACHK74_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFP0220930.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            20989..21840
                     /gene="yijO"
                     /locus_tag="ACHK74_002109"
     CDS             20989..21840
                     /gene="yijO"
                     /locus_tag="ACHK74_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP0220931.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPAPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(21827..22168)
                     /gene="frwD"
                     /locus_tag="ACHK74_002110"
     CDS             complement(21827..22168)
                     /gene="frwD"
                     /locus_tag="ACHK74_002110"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP0220932.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(22170..23048)
                     /gene="pflC"
                     /locus_tag="ACHK74_002111"
     CDS             complement(22170..23048)
                     /gene="pflC"
                     /locus_tag="ACHK74_002111"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFP0220933.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(23014..25307)
                     /gene="pflD"
                     /locus_tag="ACHK74_002112"
                     /pseudo
     CDS             complement(23014..25307)
                     /gene="pflD"
                     /locus_tag="ACHK74_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            complement(25358..25678)
                     /gene="frwB"
                     /locus_tag="ACHK74_002113"
     CDS             complement(25358..25678)
                     /gene="frwB"
                     /locus_tag="ACHK74_002113"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP0220934.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(25693..26772)
                     /gene="frwC"
                     /locus_tag="ACHK74_002114"
     CDS             complement(25693..26772)
                     /gene="frwC"
                     /locus_tag="ACHK74_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFP0220935.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            27081..29582
                     /gene="ptsP"
                     /locus_tag="ACHK74_002115"
     CDS             27081..29582
                     /gene="ptsP"
                     /locus_tag="ACHK74_002115"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP0220936.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            29594..30256
                     /gene="fsa"
                     /locus_tag="ACHK74_002116"
     CDS             29594..30256
                     /gene="fsa"
                     /locus_tag="ACHK74_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP0220937.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            30267..31370
                     /gene="gldA"
                     /locus_tag="ACHK74_002117"
     CDS             30267..31370
                     /gene="gldA"
                     /locus_tag="ACHK74_002117"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFP0220938.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATAMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            31644..32261
                     /gene="yijF"
                     /locus_tag="ACHK74_002118"
     CDS             31644..32261
                     /gene="yijF"
                     /locus_tag="ACHK74_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFP0220939.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(32288..33193)
                     /gene="yijE"
                     /locus_tag="ACHK74_002119"
     CDS             complement(32288..33193)
                     /gene="yijE"
                     /locus_tag="ACHK74_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFP0220940.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(33287..35467)
                     /gene="katG"
                     /locus_tag="ACHK74_002120"
     CDS             complement(33287..35467)
                     /gene="katG"
                     /locus_tag="ACHK74_002120"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFP0220941.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYHKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKFTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(35796..36686)
                     /gene="metF"
                     /locus_tag="ACHK74_002121"
     CDS             complement(35796..36686)
                     /gene="metF"
                     /locus_tag="ACHK74_002121"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFP0220942.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            36762..36896
                     /locus_tag="ACHK74_002122"
     CDS             36762..36896
                     /locus_tag="ACHK74_002122"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0220943.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(37035..39467)
                     /gene="metL"
                     /locus_tag="ACHK74_002123"
     CDS             complement(37035..39467)
                     /gene="metL"
                     /locus_tag="ACHK74_002123"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFP0220944.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(39470..40630)
                     /gene="metB"
                     /locus_tag="ACHK74_002124"
     CDS             complement(39470..40630)
                     /gene="metB"
                     /locus_tag="ACHK74_002124"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFP0220945.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            40907..41224
                     /gene="metJ"
                     /locus_tag="ACHK74_002125"
     CDS             40907..41224
                     /gene="metJ"
                     /locus_tag="ACHK74_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFP0220946.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            41408..42016
                     /gene="yiiX"
                     /locus_tag="ACHK74_002126"
     CDS             41408..42016
                     /gene="yiiX"
                     /locus_tag="ACHK74_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFP0220947.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            42161..42836
                     /locus_tag="ACHK74_002127"
                     /pseudo
     CDS             42161..42836
                     /locus_tag="ACHK74_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(42923..>43100)
                     /locus_tag="ACHK74_002128"
     CDS             complement(42923..>43100)
                     /locus_tag="ACHK74_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFP0220948.1"
                     /translation="ILIYKVNIINASSKKDVFFYESPTSTHNDIMGGGGMSYAYEYDV
                     KYNICHLRKKSRMR"
BASE COUNT        10666 a        11860 c        10735 g         9839 t
ORIGIN      
        1 ctttttctca gcggcgcggg gtgtgcataa tacgctttcc cgctacagag tcaagcattt
       61 atttttgctt tctctgtcgg agttctcagg agaaccccgc tgacccggcg gcgtgtttgc
      121 cgttgttccg tgtcagtggt ggcgcattat agggagttat tccggcctga caagaggaaa
      181 tttaaaataa ttttctgacc gcgcaacatt caaccaaatc agcctaaaac tgccagtttt
      241 tcgagcgttt cgaagccgta acgctgtaaa acgggcaata attgttcagc ttctggcgtc
      301 attgccatac aaaaggcaat attcgcatcg gcagattttg catccggagc ttcatgttct
      361 aacagccagg ccgttctgcg agcaatcgct gcgccagaat ccaccagccg ggttccctct
      421 ggcagcacct gtaagagttc ttcttgtagt agagggaaat gggtgcaacc caatacaacg
      481 gtatctggcg gctctttcat tcttaaccac gggcgtagga tacgttttag tgcatccaga
      541 gaaacatctt cgccatgcag cttcgcttca gccaactcaa ccatctctgc cgagcccagc
      601 atttctatct ggcattcatt agcgaaacgc gcgatcagct catgagtata agaacgttta
      661 acagttccgc gggttgccag taatccgaca atgccatttg ccgtcagacg tgcagcaggt
      721 ttaatcgccg gcacgacacc aacaaccggg aagtcgaact tttcgcgtaa tgcaggaagt
      781 gaaacggtac tggcggtgtt gcaagcgacc acagccagcg caaggggata acgttcttgc
      841 actgcggtga caattgccac cactcgctca acaataaact cttcgctttt ttcgccatac
      901 ggaaaagcga cgttatcgaa agcataaatg taatggagat ccggtaagag atgccggatc
      961 tcgtcataga ccgacaaccc accgacgccg gagtcaaaca ccagcacggt gggacgtggt
     1021 tcagaaggtg tagctgccag acaaggtgta ttcccgtcct gcagtttggt agccatagac
     1081 tgtctcataa tctttgtcga acaggttggc tattttacca cgaactgtca ggtgagaggt
     1141 gaccggatac gcaaccgcaa gatcccacaa gctcacaccg cccattttaa cggtttgata
     1201 aggataagat gagtaatcct tatcatagcg agtgcctaaa tactgataag taatacccca
     1261 gtcgaagtca tacaactgcc agtcgagctg gtatttcacc tgctgtttag cacggcgtaa
     1321 caacggcgtg tcggtaatcg cattgcgcgc atcgacataa tcataactca cagtatgcgt
     1381 cagtggtccg gtatcaaaat tggcggtcgc ctcgacgccc ttaatccgcg ctttcccttc
     1441 gttgtaatat ttcagggtgt gatcatcata atcgatcaag tcactgacat cgttacgata
     1501 tccggaaata cgccagttca ccccagcggt taagccttca aacgcgcctt cccactgttt
     1561 gcttttctcc gggtccagat tcggatttcc gtagaagcca tacagttgcc ccagatttgg
     1621 tgccttataa gatgtcccgt aggaagcaat gaagcgataa ccttcgatga attcccaacc
     1681 ggcgctggtt tgccaggttc catgacgacc aaactgtgag ttatcgtcac tgcgtgctgc
     1741 gccttcaaag gtaaaatcgc cgacttgttg cagcccggtc agatagatgc cggtattacg
     1801 ttgatcatat ccattctcaa cataacctgt acccggcgtc gtagtctgtt tctgccagtc
     1861 gacacccgca ccaatactac cgtgaccaac gatgacattg tttgcccact ggacggtgta
     1921 ttgcttcatc tcatcgagcg tcgccgacga atcataacga ccaaaatggg gatcgtagtt
     1981 gtaatctttg ctatggctat agctggtaat gagttgtgat ttaatcagtt cgccgttata
     2041 gcgcagcccg gcgtcccaac tttggctata gagtttacgg gtatcgagca acggtgaacc
     2101 gggagaataa tacgcgtcat aattggtacg gttatcatag ccatagccgc gcacaaagcc
     2161 gctccaggca tcagtaaagt tatgctccag cgcgccataa agcgttttac ttaaaaaacc
     2221 atcgttatct gtctgcgctt gcgttccggt attaccatag gcaacaacat cataaccatg
     2281 agtatgggca taatcgccca acagcgttac ccgtgtctta tcccccagtt gttgctgcgt
     2341 agagacatca tagttctgat aactattgct tccccaccct gctgaaattt ccgttccggg
     2401 ttcatcgcgc gtcgtgatga tattcaccac cccgcctatt gcatcggaac cataaacagc
     2461 ggagcgcggc ccacggatat attcaacacg ctggacaagc gcaataggta actggctaag
     2521 gtcggcagaa ccactcaccc ccgccagatt caggcgtacg ccatcaatta acaccaacac
     2581 atgactggca tttgtaccgc gaataaaaat agatgagagc tgacctgaac cgccgttttg
     2641 ggtgatatcg acgcccggaa gacggcgcag cacatcattg accgaggtcg actgccagcg
     2701 gtcgatatcc tgacgggtca caacggtggt tggtgcaagc acagtgctgc gcggctgttc
     2761 aaaacggtta gcagtaacga cgagagtatc cgggctggta tcctgtgccc aagcggaaaa
     2821 ggctgtgaca gaacacgccg tcagcagcga agctttttta atcattgtaa agcatccaca
     2881 atagaagaag gatgccgcag gtttcatcaa tattacgcga tgatgagaac cagatgcgac
     2941 gttggccggc aggtcttcgg gcttggaggg gtatctaaga tactaagaga tgatgacttc
     3001 ccaccggatg gcagtgtccg cataacgcaa tcatcgcacc tttccttacc gctgcgcgtc
     3061 agctccagat tcgcactgga ttccctatta actcacagga ccggcaagtg gatgctacag
     3121 gttgtaacaa gttactgtcc agacgtagct cacaaatagg aattcatcaa gatctggaca
     3181 tctgatgagc aatccctaca atcgccgcgt actttaattt ttcaggatac atcatgaccc
     3241 ccgaacacct tccaacagaa cagtatgaag cgcagttagc cgaaaaagtg gtacgtttgc
     3301 aaagtatgat ggcaccgttt tctgacctgg ttccggaagt gtttcgctcg ccggtcagtc
     3361 attaccggat gcgtgcggag ttccgcatct ggcacgatgg cgatgacctg tatcacatca
     3421 ttttcgatca acaaaccaaa agccgcatcc gcgtggatag cttccccgct gccagtgaac
     3481 tcatcaacca gttgatgacg gcgatgattg cgggtgtgcg taataatccc gttctgcgcc
     3541 acaagttgtt ccagattgat tacctcacca cgctgagtaa tcaggcggtg gtttccctgt
     3601 tgtaccataa aaagctggat gatgagtggc gtcaggaagc ggaagccctg cgcgatgcac
     3661 tgcgcgcgca gaatctgaat gtgcatctga ttggtcgggc aacgaaaacc aaaatcgagc
     3721 tggatcaaga ttacatcgat gaacgtctgc cggtcgcagg gaaagagatg atctaccgtc
     3781 aggtagaaaa cagttttacc cagccgaacg cggcgatgaa tattcagatg ctggaatggg
     3841 cgctggacgt aaccaaaggc tcaaaaggcg atttactgga gctgtactgc ggcaacggta
     3901 acttttcatt agcactggcg cgcaattttg atcgggtatt agccaccgaa atcgctaagc
     3961 cgtcggttgc tgctgcgcaa tacaacatcg cagctaacca tattgataac gtacaaatta
     4021 ttcgtatggc ggcagaagaa tttactcagg cgatgaatgg tgtacgcgag tttaatcgcc
     4081 tgcaagggat cgacttgaag agttatcagt gcgaaaccat ttttgtcgac ccgccgcgca
     4141 gcggtctgga cagtgaaacc gagaaaatgg tgcaggcgta tccacgtatt ctgtatatct
     4201 cctgcaatcc ggaaacgtta tgcaagaatc tggaaacatt aagccagacg cacaaggtcg
     4261 aacgtctggc tctgtttgat cagttcccct acacgcacca tatggagtgc ggcgtattac
     4321 tgaccgcgaa gtaaaaccca tgccggatgc gccagcatcc ggcatgattc cgattactca
     4381 gcaacttcct gcttacggtt acgcatcttc gcgccaatcc agaacaccat aatgacggag
     4441 agtactgccg ggaagaagtt agagccgata tccggatatt ccgcacgaac taccgtgctg
     4501 tacagcagta cgccgagaat aaaacaggca gctgccagac ccggcaagcc taccggcata
     4561 gtgcgattaa gataacgttg atgcaggcag taaaccgtca gcaccaggga aataatcggg
     4621 aatacagaaa atggcacaat ggagctaaac agcgctgcga aagtaccatt aatcgataag
     4681 ccagcaacta acgccagcag cagcgtacct ctatcttgat ttgcttgttt cattactcgt
     4741 ccttcacatt tcccggaata attgcggttt tctcttgttc acggcgatac cagtaataag
     4801 cccctttcga aatcattcgc agttgcagta ccagtcgctc ttctaattgc cgacgttgtt
     4861 cgacgccgat gtccaacgcc tcggcacccg cactgaagac aattgtcacc attgcttcgg
     4921 cttgcgcttc agtaaacgca cgcggcatat ggttttcgag ttccagatag tccgcaagtt
     4981 ccgcaatgaa gtgctgaatt tcacgcgcaa cggcggcacg aaacgcagcg gaggtgccgg
     5041 agcgttcccg caataataac cggaaggcgt taggattatt accgatgaac tccataaatg
     5101 tggagaccga ggtgcggatc acactcccgc ctttggcgat acgctgacgc gcctggcgca
     5161 tgagttggcg tagcattaaa ccgctctcat caaccatggt cagacccagt tcgtctacgt
     5221 cgcggaaatg ccgataaaaa gaggtgggag caatgcccgc ttcacgcgcc acttcacgca
     5281 aactcaggct ggcgaagctg cgttcagcac ttaattggct aaatgcggct tccaccagcg
     5341 aacggcgggt tttttctttt tgttgcgctc ttacgcccat cacgatgtct gaatccttgc
     5401 caaaagtact gctggcacta taccagagaa tgaacatact ggattgctcc agtattgtga
     5461 atgaacggta acgcaataaa acatatttac tgcaattctt tggccggttc ttttacgtac
     5521 agcggaaacc tgccgcttaa acggagagta tcgtcgataa aaatccaata aaacgtcagg
     5581 gcaaaagtaa gaaacagaca aagcaaaggc cgctcaggat atagccagat aaatgacggg
     5641 gatcaattgg cttacccgcg ataaaatgtt accattctgt tgcttttatg tataagaaca
     5701 ggtaagccct accatgccac attcctacga ttacgatgcc atagtaatag gttccggccc
     5761 cggcggcgaa ggcgctgcaa tgggcctggt taagcaaggt gcgcgcgtcg cagttatcga
     5821 gcgttatcaa aatgttggcg gcggttgcac ccactggggc accatcccgt cgaaagctct
     5881 ccgtcacgcc gtcagccgca ttatagaatt caatcaaaac ccactttaca gcgaccattc
     5941 ccgactgctc cgctcttctt ttgccgatat ccttaaccat gccgataacg tgattaatca
     6001 acaaacgcgc atgcgtcagg gattttacga acgtaatcac tgtgaaatat tgcagggaaa
     6061 cgctcgcttt gttgacgagc atacgttggc gctggattgc ccggacggca gcgttgaaac
     6121 actaaccgct gaaaaatttg ttattgcctg cggctctcgt ccatatcatc caacagatgt
     6181 tgatttcacc catccacgca tttacgacag cgactcaatt cttagcatgc accacgaacc
     6241 gcgccatgta cttatctatg gtgctggagt gatcggctgt gaatatgcgt cgatcttccg
     6301 cggtatggat gtaaaagtgg atctgatcaa cacccgcgat cgcctgctgg catttctcga
     6361 tcaagagatg tcagattctc tctcctatca cttctggaac agtggcgtag tgattcgtca
     6421 caacgaagag tacgagaaga tcgaaggctg tgacgacggt gtgatcatgc atctgaagtc
     6481 gggtaaaaaa ctgaaagctg actgcctgct ctatgccaac ggtcgtaccg gtaataccga
     6541 ttcgctggcg ttacagaaca ttgggctgga aactgacagt cgcggacagc tgaaggtcaa
     6601 cagcatgtat cagaccgcac agccgcacgt ttacgcggtg ggcgacgtga ttggttatcc
     6661 gagcctggca tcagcggcct atgaccaggg gcgcattgcc gcgcaggcgc tggtaaaagg
     6721 cgaagccacc gcgcatctga ttgaagatat ccctaccggc atttacacca tcccggaaat
     6781 cagctctgtg ggcaaaaccg aacagcagct gaccgcgatg aaagtgccat atgaagtggg
     6841 ccgcgcccag tttaaacatc tggcacgcgc acaaatcgtc ggcatgaacg tgggcacgct
     6901 gaaaattttg ttccatcggg aaacaaaaga gattctgggt attcactgct ttggcgagcg
     6961 cgctgccgaa attattcata tcggtcaggc gattatggaa cagaaaggtg gcggcaacac
     7021 tattgagtac ttcgtcaaca ccacctttaa ctacccgacc atggcggaag cctatcgggt
     7081 agctgcgtta aacggtttaa accgcctgtt ttaacacttt atcgaaatgg ccatccattc
     7141 ttgctcggat ggcctctgcc agctgctcat agcggctgcg cagcggtgag ccaggacgat
     7201 aaaccaggcc aatagtgcgg cgtggttccg gcttaatgca cggcagataa acaaccccat
     7261 cgcgtttgcg ctccggcggc acagccagcg ctggcagtaa agtgatcccg ctacctgccg
     7321 ccaccatgtt gcgcagcgtc tccaggctgg tcgcgcggaa gtgtgtatct tcatccgccc
     7381 cggcttcaaa acagaaaccc attgcctgat cgcgcaaaca gtgaccatct tccagcatca
     7441 gcagtttttc ccctgccaga tcggccatcg gtacgcattc gcggttcgcc cacgggtgat
     7501 cttcatagat agccagcaac attggctcat caaacaacgg cacttcaatg aatgcttcgc
     7561 tctctttcac cagcgcgagg atcacgcaat cgagtttgcc gctgtccagt tgcgccagta
     7621 actggtgggt ctgtgcttca tgcagataca tttccagctt tggaaaggtc tggtgcagca
     7681 tagggataat atgcggtagc aggtacggtc caactgtggg aatcaaacca atgtgcagcg
     7741 gcccggacat cgtctcgccc tgctggcttg ccatctcttt aaggactttc acctcacgca
     7801 gtacggtacg cgcctgatct accagcagca ttcccgcctg ggtgaacaac actttacggc
     7861 tggtccgctc cagcaacatc acgcccaact catcttccag cttacgaatt tgcccgctaa
     7921 gcgtcggctg gctaacgtgg caggaatctg ccgcacgccg aaaatggcgg tgctcggcca
     7981 atgccaccag gtactcaaga tcacgaatat tcattatcca tcctccatcg ccacgatagt
     8041 tcatggcgat aggtagaata gcaatgaacg attatcccta tcaagcattc tgactgataa
     8101 ttgctcacag aaacggagcg gcacctcttt taacccttga agtcactgcc cgtttcgaga
     8161 gtttctcaac tcgaataact aaagccaacg tgaacttttg cggatctcca ggatccgctt
     8221 ttttttattc tacaggccca cagcaagaga gcctgatagt aacgattact caatgcgatc
     8281 tttgacaata aagaagtaac acagcgcccc ggtgaacgtc acacaggccg cgataaccaa
     8341 cgccagatta aacgagtgtg tggtatccac tacccaaccg gttacgatcg gagcaaagga
     8401 agcaaaaaca aaactgccaa agttttgaat cccggcgatg gaagccactt ttgtttctga
     8461 caccatcacc tgcaccagcc cccatgcaga cgttccagcg aaatgcacgc agaatagcgc
     8521 cattgagata aacgctaccg cctgggctgg cgaggaagat tgcacgacca gcaacgtgcc
     8581 taatgccgac atcatcaaac cgcagacaat agccgtttta cgcgtcttcg ccaggtcgta
     8641 gccttttttc gccagtcgat cgacaacaat accgttaacc cacatcccga ctgccgccgc
     8701 gaggaaagga atcgccgcca cccaaccggt tttcgccaga ctgaaacctt gctcggcttg
     8761 caaataaccg ggtaaccacg cgatgtagag ccaaccggta taattgacgc cagagaaacc
     8821 taaaatcatc ccccaggttg tccgatgctt aaacagcgcc agccactcgc taaattgcag
     8881 ctgtggacgc ggtttaaccg gcgcggagag atacgtccgt tcttcgtcag tcaggacaaa
     8941 ctgcgcccgg ttgcgatacc acgcgtacca acagatccca accagaatcc ctgccacgcc
     9001 gatgataacg aacatcgtcc gccagcccca tgccagctgc atcaatacaa gagcaggagg
     9061 cgcgatagcc tgaccgataa cggtagatga gttaaaaatc cccagcgccg tgccgcgttc
     9121 tttttgtgca taccagtcgg tgatcgactt tactcccgca ggcataaatg gcgcttcgcc
     9181 aatacccaga ccgatacgca ttaaaataaa gtggctaaaa gaattaacca ttcctgttaa
     9241 tgcctgcatt aatgaccaga atattaagcc tgcgcctaaa acaattcgtg ggccaaagcg
     9301 gtctagcaaa ataccggaag gtagttgtga aaaaccataa gacagagaaa atgcagaaag
     9361 caaaacacca aactctgtcg ccgataatcc taactcgcca cgaatcgctt cacctgccac
     9421 gctcaatgac gaacggtcga gaaaattaac gatccccgcc ataaataata aaaccagggt
     9481 cactgtctga atacgacgaa tccgttgcgg tttattaccc gcaacactgg ttgacacatc
     9541 aacgtccatt tttagctcct tgagcggaat taatcgattt gatgactatt cagaaaataa
     9601 ttaagcgaac cgttattacc agttccacaa tgtgccatct tccagtcgtg cgacggggag
     9661 atacgccggg tcatacggat atttcgccgc cagtttctca tcgaaagaaa tacccagccc
     9721 cggttcatcg cctgggtgca tataaccttc ctcgaagcgc cagctgtgcg ggaaaacttc
     9781 aagcatttgt tcggaatagc ccatgtactc ctgaacgcca aaatttggta cccataggtc
     9841 aaaatgcagc gccgccgcat gacaaacagg cgataaatct gaagggccat gcgaaccggt
     9901 acgtacctga taaagcgatg caaaatcggc aatccgccgc atcccggtaa tgccgcctgc
     9961 atgggtgatc gtcgtgcgaa tatagtcaat cagctgctct tcgattaact gcttgcagtc
    10021 ccagatactg ttaaacactt caccgaccgc aatcggcgtc acggtatgct ggcgaatcag
    10081 acgaaaacat tcctggttct ctgcaggcgt cggatcttcc atccagaaca ggcggtaatc
    10141 ctcaatgctc ttcccaaagc gcgccgcttc gatgggggtc aggcgatggt gcatgtcatg
    10201 caacagatgt tcgttaaaac cgaacttatt acgcaccgct tcaaataact tcggcgtaaa
    10261 atcgagatac ttttctgaag accacaactg ctcttctggc cacaggcctt tggttgccgg
    10321 ttcatacgcc aggcctttgc ccttcgccag gccataagta gtttgcatac ccggtacacc
    10381 gcactgcaca cgaatcgcct taaagcccat ctgctggtgc tttgcatagt cttcaaggac
    10441 ttcatcaatc gtccgcccgg tggtgtggca ataaaccatc acgccttcac gagaagcacc
    10501 accaagcagt tgatacaatg gcatatttgc cgctttagct ttgatatccc acaacgccat
    10561 atcaatggca gaaatggctg acatcgtgac ggggccacgt cgccagtaag cacctttata
    10621 aaagaattgc cagatatctt cgatacggct ggcatcgcgg ccaattagtt gtggacaaag
    10681 atgatctttc agataagaag cgaccgacaa ttcgcgacca tttaacgtgg catctcctaa
    10741 tccacaaagt ccgctttcag tggttatttt aagcgtaacg aaattacgcc ctgggcaagt
    10801 aacaaaaaca tctgcagcaa taattttcat tattgcaatc tcctgtgttg taaatagacg
    10861 cttcaaactt aattttacgc ctactaccat acaagagaga aagagcgatt attcaaccgt
    10921 gatcacatta aaaataaaaa tttccgccga ggagaaaaaa taccctcaaa aaccacctga
    10981 ataattaacc accatcgtta aaacaacacg ttaaataatc acataacaaa ctccattaat
    11041 cattaaaaat aagcacaaca atagaaatga attatattca attaattaaa atatgtattg
    11101 attgatgtat tgaaaatatg atttttatgg ggagtgtgaa ggtaaagaag acacaatgat
    11161 aaaaaggaat gtgacaaata atggttctgg ttgggcgtta aatttaagct caccagaatg
    11221 ctgacaaatt tctgcctgat gtggcgtaaa cgccttatcc gcctacgaag tgtcgcactt
    11281 tgtaggcgtg gtaagctccc cattttcagg ggagcaatat aaacaaatta ctctaaccgc
    11341 gcctgcgcaa aagcaattgc ctgcgccacc tgctgcggtg agacgccgcc ttttgccgca
    11401 cgcttgtcga ggcacgattg cagcgacaga atcggataga catcttcgtc aatcaccggg
    11461 ctgaatttct gcaactcgtc gagcggcaga tcttccagcg gtttgccctg acgaatggct
    11521 tccaccaccg cttcaccaac aatatggtgc gcctcgcgga acggtacgcc tttcgccacc
    11581 agataatccg ccagttcggt ggcgttggcg taaccctgct gagccgcttc ttggcaacgt
    11641 ggacgtttca cctgaatgcc gtccagcacc agcgccgcca tatgcaggca gtccagccag
    11701 gtatcgagcg cgtcgaacag accttctttg tcttcctgca tatctttgtt gtaagccagc
    11761 ggcaaacctt tcagcgtcat catcatgccg gttaacgccc cctgcacccg accgcattta
    11821 ccgcgaatca gctccagcgc atccgggttt ttcttctgcg gcattaatga tgaaccggaa
    11881 gtcacgcggt cagaaagctc cacaaacccc gcttcgccgg tgttaaagaa aatcagatct
    11941 tcagcaaaac gcgacagatg caccatgccg atagcggcag ccgacagcag ttccaacaca
    12001 tggtcacggt cagaaacgct gtccagactg ttacgggtcg ccgaagcaaa gcccagccag
    12061 cctgctaact gttcacggtc gatttcatag gccgttcccg ccagcgcgcc acagcctaat
    12121 gggctgacat ccagacgctt aagcgcatcc tgcaaacggc tttcatcacg cgccagcatc
    12181 tcaacatagg ccaggcacca gtgcgcgaac gtcaccggct gggcgcgttg caggtgagtg
    12241 taacctggca tgaccgcgtc ctgattgttt tgtgcggttt ccaccagcgc cgattgcagc
    12301 tgtcggttag ccgtcagtaa ctcgctaacg gtatctttgc accacagttt caggtcagtc
    12361 gctacctgat cgttacgact acgcccggta tgcagctttt tgcctaactg gcccactttg
    12421 tcgatcagtt tgccttccac ccagctatgg atatcttcgg cgtcgctttc aaggatttgt
    12481 tgtggcctgg cgcgaacatc ttccagcagc acgttcagcg cctcttccag ttgcgcctgc
    12541 tcttctgcgg ttaacacgcc taccgtgacc agggctttgg accaggccac agagccaaca
    12601 atatcctgct ccgccagacg gtaatcaaag cgcagtgagt cgttgaattg tttgaaccgt
    12661 tgatctgctg cctgggtaaa acgcccgccc caaagtgcca taactctgtt tccttatttt
    12721 gaaattcaat gccggaaagc tgcgcctccg gccaacaaaa cttaagctaa aatccgcgta
    12781 cccatcggca taccgttaaa cagtgccgga agctgctccg catgacgcca ggaggcgata
    12841 tctaccggac ggcccagcgt gcgggccgca tccagcgccg cgttcacttt cactatcatg
    12901 ccgtcagtaa taatgccctg ctcaatcagt tgttctgctt tcgcggcggt catttcggca
    12961 atgcgttgcc ctttgccgtc gagaatgccg ctgacgtcgg agagcaaaat cagatccgcg
    13021 cccagcgttg ccgccagcgc cgttgccgcc tggtcggcat tgacgttcat cagttgccct
    13081 tcgtctgtta cgccaatgga gctgaccacc ggcagataac cgttctccag caaggagttg
    13141 ataagcttag gcgaacctgg ctgcgccagt ccaacatgac ctaactcttc atcaagctgg
    13201 gtcactttga cgctgtcgcc gtcaccgaga aacaaaccta cggccgcaat ctgatgtttc
    13261 ttcgcccatg ccaacagggt tttatttgcc gttcccgcca gtgctccggt gataatgtct
    13321 atctgatcag caggcgtcac ccgcaggccg tttttctttt tcaccggcag attcagccct
    13381 ttcatcagct catccaccac gcaaccgccg ccgtgcacaa tcaccagcgg acgctgatgt
    13441 gactcacgat aattcaccag tgcgctaaac agacgttcca gcgcctcttc actatccagc
    13501 agtacgccgc ccagtttgat aattaatgga ttcatcattg cacccttaaa taagagactg
    13561 cgtttccgca tagccgaaac gaatattggc gcactgtacc gcttgtgccg ccgcgccttt
    13621 cagtaagttg tcttcggtcg ccacaataat cagatgctcg ccctgaacgg caaacccgat
    13681 atcgcaaaat ggcagcccaa cgacattttt cagcgccgga acgcctttgt catacagccg
    13741 caccagcggt ttatgggcat acgcctgctg taacacttgc gcgacttgcg cctgggtcac
    13801 acccgatttc aggcggcagg taatggtttc gagaatgccg cgcgggaaat tgcccagatg
    13861 tggggtgaag ataacgtcag caccgaggtg tgtggcgatc tctggttgat ggcgatgagt
    13921 aaagacgcca tacggttgca ggctaacttc acaaaagctg tttgaaatgg ccgctttacg
    13981 ccctgcaccg ctcacgccgc tggtggcgtt gatcaccggc cactgattga ggtcaagaag
    14041 atcggcatca atcaacggtt tcagcgccag ctgtgccgcc gtcggataac agcccggcac
    14101 cgcaatcaaa ttcgcttctt ttaatttatt accgcaccac tccgccagac cgtaggctgc
    14161 ctgttccaac agttccgggt attgatgggt aaagccgtaa tatttttcat agaaggtggc
    14221 gtcgttaaca cgaaacgcgc cggaaaggtc gaacaccacg cagcccgctt caagaaactg
    14281 cggcgctaaa tcgtggctaa cttcatgggc ggtggcgaga aacactacgt ccacccctgg
    14341 gctaaactcg ctgatatccg acatcggctg caacggcaga tcaacgatgc cttttagctg
    14401 cggatgcaaa tcggagatta actttcccgc atcattgctt tgcgctgaaa cagtcaaagc
    14461 ggttatgttc atatgcggat ggcgatttac ataggtcact agctctgcgc cagcgtagcc
    14521 gctggcaccc acaatcagcg tattcaacat cggggctatt caccttctta tgtctggttg
    14581 ccaggttaaa cgtaaaacat tcaccttacg gctggtgggt tttattacgc tcaacgttag
    14641 tgtattttta ttcataaata ctgcatgaat attgatacta tcatgaccag aggtgtgtca
    14701 acaatgaaaa acaaattacc gccatttatc gagatttacc gcgctctgat tgccacacct
    14761 tcaataagcg ccacggaaga ggcactcgat caaagcaatg cagatttaat cactctgctg
    14821 gcggactggt ttaaagattt gggcttcaat gtggaagtgc agcctgttcc aggaactcgc
    14881 aacaaattta atatgctggc cagttgcgga cagggggctg gcggcttgtt gctggcgggg
    14941 cataccgata cggtgccatt tgatgacggt cgctggacgc gcgatccgtt tacactgacg
    15001 gagcatgacg gcaagcttta cggcttaggc accgccgaca tgaaaggctt ttttgcgttt
    15061 atccttgatg cgctacgcga tgtcgacgtc acgaaactga aaaaaccgct ctacattctg
    15121 gcgactgctg atgaagaaac cagtatggcc ggagcgcgtt attttgccga aactaccgcc
    15181 ctgcgcccgg attgcgccat cattggcgaa ccgacgtcac tacaaccggt acgcgcacat
    15241 aaaggtcata tctctaacgc catccgtatt cagggccagt cggggcactc cagcgatcca
    15301 gcacgcggag ttaacgctat cgaactaatg cacgacgcca tcgggcatat tttgcaattg
    15361 cgcgataacc tgaaagaacg ttatcactac gaagcgttta ccgtgccata ccctacgctc
    15421 aacctcgggc atattcacgg tggcgacgct tctaaccgta tttgcgcttg ctgtgagttg
    15481 catatggata ttcgtccgct gcctggcatg acactcaatg aacttaatgg tttgctcaac
    15541 gatgcattgg ctccggtgag cgaacgctgg ccgggtcgtc tgacggtcga cgagctgcat
    15601 ccgccgatcc ctggctatga atgcccaccg aatcatcaac tggttgaagt agttgagaaa
    15661 ttgctcggag caaagaccga agtggtgaac tactgtaccg aagcgccgtt tattcaaacg
    15721 ttatgcccga cgctggtgtt ggggcctggc tcaattaatc aggctcatca acctgatgaa
    15781 tatctggaaa cacggtttat caagcccacc cgcgaactga taacccaggt aattcaccat
    15841 ttttgctggc attaaaacgt aggccggata aggcgctcgc gccgcatccg gcactgttgt
    15901 caaactccag tgccgcaata atgtcggatg cgatacttgc gcatcttatc cgacctacac
    15961 ctttggtgtt acttggggcg attttttaac atttccataa gttacgctta tttaaagcgt
    16021 cgtgaattta atgacgtaaa ttcctgctat ttattcgttt gctgaagcga tttcgcagca
    16081 tttgacgtca ccgcttttac gtggctttat aaaagacgac gaaaagcaaa gcccgagcat
    16141 attcgcgcca atgcgacgtg aaggatacag ggctatcaaa cgataagatg gggtgtctgg
    16201 ggtaatatga acgaacaata ttccgcattg cgtagtaatg tcagtatgct cggcaaagtg
    16261 ctgggagaaa ccatcaagga tgcgttggga gaacacattc ttgaacgcgt agaaactatc
    16321 cgtaagttgt ccaaatcttc acgcgctggc aatgatgcta accgccagga gttgctcacc
    16381 accttacaaa atttgtcgaa cgacgagctg ctgcccgttg cgcgtgcgtt tagtcagttt
    16441 ctgaacctgg ccaacaccgc cgagcaatac cacagcattt cgccgaaagg cgaagctgcc
    16501 agcaacccgg aagtgatcgc ccgcaccctg cgtaaactga aaaaccagcc ggaactgagc
    16561 gaagacacca tcaaaaaagc agtggaatcg ctgtcgctgg aactggtcct cacggctcac
    16621 ccaaccgaaa ttacccgtcg tacactgatc cacaaaatgg tggaagtgaa cgcctgctta
    16681 aaacagctcg ataacaaaga tatcgctgac tacgaacaca accagctgat gcgtcgcctg
    16741 cgccagttga tcgcccagtc atggcatacc gatgaaatcc gtaagctgcg tccaagcccg
    16801 gtagatgaag ccaaatgggg ctttgccgta gtagaaaaca gcctgtggca aggcgtaccg
    16861 aattacctgc gcgaactgaa tgaacaactg gaagagaatc tcggctacaa actgcccgtc
    16921 gaatttgttc cggtccgttt tacctcgtgg atgggcggtg accgcgacgg caacccgaac
    16981 gtcactgccg atatcacccg ccacgtcctg ctgctcagtc gctggaaagc caccgatctg
    17041 ttcctgaaag atattcaggt tctggtttct gaactgtcga tggttgaagc gacccctgaa
    17101 ctgctggcgc tggttggcga agaaggtgcc gcagaaccgt atcgctatct gatgaaaaac
    17161 ctgcgttctc gcctgatggc gacacaggca tggctggaag cgcgcctgaa aggcgaagaa
    17221 ctgccaaaac cagaagggct gctgacacaa aacgaagaac tgtgggaacc gctctacgct
    17281 tgctaccagt cacttcaggc gtgtggcatg ggtattatcg ccaacggcga tctgctcgac
    17341 accctgcgcc gcgtgaaatg tttcggcgta ccgctggtcc gtattgatat ccgtcaggag
    17401 agcacccgcc ataccgaagc gctgggcgag ctgacccgct acctcggtat cggcgactac
    17461 gaaagctggt cagaggccga caaacaggcg ttcctgatcc gcgaactgaa ctctaaacgt
    17521 ccgctgctgc cgcgcaactg gcaaccaagc gccgaaacgc gcgaagtgct cgatacctgc
    17581 caggtgattg ccgaagcgcc gcaaggctcc attgccgcct acgtgatctc gatggcgaaa
    17641 acgccgtccg acgtgctggc tgtccacctg ctgctgaaag aagcgggtat cgggtttgca
    17701 atgccggttg ctccgctgtt tgaaaccctc gatgacctga acaacgccaa cgatgtcatg
    17761 acccagctgc tcaatattga ctggtatcgt ggcctgattc agggcaaaca gatggtgatg
    17821 attggctatt ccgactcagc aaaagatgcg ggagtgatgg cagcttcctg ggcgcaatat
    17881 caggcacagg atgcattaat caaaacctgc gaaaaagcgg gtattgagct gacgttgttc
    17941 cacggtcgcg gcggttccat tggtcgcggc ggcgcacctg ctcatgcggc gctgctgtca
    18001 caaccgccag gaagcctgaa aggtggcctg cgcgtgactg aacagggcga gatgatccgc
    18061 tttaaatatg gtctgccaga aatcaccgtc agcagcctgt cgctgtacac cggggcgatt
    18121 ctggaagcca acctgctgcc accacctgag ccgaaagaga gctggcgtcg cattatggat
    18181 gaactgtcag tcatctcctg cgatctctac cgtggctacg tacgtgaaaa caaagatttt
    18241 gtgccttact tccgctccgc tacgccggaa caagaattgg gcaaactgcc gttgggttca
    18301 cgtccggcga aacgtcgccc aaccggcggc gtcgagtcgc tgcgcgccat accgtggatt
    18361 tttgcctgga cgcaaaaccg cctgatgctc cccgcctggc tgggtgcagg tacggcgctg
    18421 caaaaagtgg tcgaagatgg caaacagagc gagctggaag ccatgtgccg cgattggcca
    18481 ttcttctcga cgcgtctcgg catgctggag atggtcttcg ccaaagcaga cctgtggctg
    18541 gcggaatact atgatcaacg tctggtagac aaagcactgt ggccgttagg taaagagtta
    18601 cgcaacctgc aagaagaaga catcaaagtg gtgctggcga ttgccaacga ttcccatctg
    18661 atggccgatc tgccgtggat tgcagagtct attcagctac ggaatattta caccgacccg
    18721 ctgaacgtat tgcaggccga gttgctgcac cgctcccgcc aggcagaaaa agaaggccag
    18781 gaaccggatc ctcgcgtcga acaagcgtta atggtcacta ttgccgggat tgcggcaggt
    18841 atgcgtaata ccggctaatc ttcctcttct gcaaaccctc gtgcttttgc gcgagggttt
    18901 tctgaaatac ttctgttcta acaccctcgt tttcaatata tttctgtctg cattttattc
    18961 aaattctgaa tataccttca gatatcctta aggaattgtc gttacattcg gcgatatttt
    19021 tttcaagaca ggttcttact atgcattcca cagaagtcca ggctaaaccc ctttttagct
    19081 ggaaagccct gggttgggca ctgctctact tttggttttt ctctactctg ctacaggcca
    19141 ttatttacat cagtggttat agtggcacca acggcattcg cgactcgctg ttattcagct
    19201 cgctgtggtt gatcccggta ttcctctttc cgaagcggat taaaattatt gccgcagtaa
    19261 tcggcgtggt gctatgggcg gcctctctgg cggcgctatg ctactacgtc atctacgggc
    19321 aggagttctc gcagagcgtt ctgtttgtga tgttcgaaac caacaccaac gaagccagcg
    19381 agtatttaag ccagtatttc agcctgaaaa ttgtgcttat cgcgctggcc tatacggcgg
    19441 tggcagttct gctgtggaca cgcctgcgcc cggtctatat tccaaagccg tggcgttatg
    19501 ttgtctcttt tgccctgctt tatggcttga ttctgcatcc gatcgccatg aatacgttta
    19561 tcaaaaacaa gccgtttgag aaaacgttgg ataacctggc ctcgcgtatg gagcctgccg
    19621 caccgtggca attcctgacc ggctattatc agtatcgtca gcaactaaac tcgctaacaa
    19681 agttactgaa tgaaaataat gccttgccgc cactggctaa tttcaaagat gaatcgggta
    19741 acgaaccgcg cactttagtg ctggtgattg gcgagtcgac ccagcgcgga cgcatgagtc
    19801 tgtacggtta tccgcgtgaa accacgccgg agctggatgc gctgcataaa accgatccga
    19861 atctgaccgt gtttaataac gtagttacgt ctcgtccgta caccattgaa atcctgcaac
    19921 aggcgctgac ctttgccaat gaaaagaacc cggatctgta tctgacgcag ccgtcgctga
    19981 tgaacatgat gaaacaggcg ggttataaaa ccttctggat caccaaccag cagacgatga
    20041 ccgcccgcaa taccatgctg acggtatttt cgcgccagac cgacaagcag tactacatga
    20101 accagcaacg tacgcagagt gcgcgtgaat acgacaccaa cgtgctgaag ccgttccagg
    20161 atgtgctgaa tgaccctgcg ccgaagaaac tgatcatcgt tcatctgctg ggtacgcata
    20221 tcaaatacaa ataccgctac ccggaaaatc agggcaagtt tgatggcaat accgatcatg
    20281 ttccgccagg attaagcgca gaagagctgg aatcatataa cgattatgac aacgctaacc
    20341 tgtataacga tcatgtggtt gccagcctga ttaaagactt taaagcggca gacccgaacg
    20401 gattcctggt ttacttctct gaccacggtg aagaggttta cgacacgccg ccgcacaaaa
    20461 ctcaggggcg taacgaagac aacccgacgc gccacatgta caccattccg ttcctgctgt
    20521 ggacgtcgga aaaatggcaa gcgactcatc cccgtgattt ctcacaggat gtcgatcgta
    20581 aatacagcct ggcggaactg atccacacct ggtcagattt ggcgggctta tcttacgacg
    20641 gttacgatcc aacccgttca gtggtgaatc cgcagttcaa agaaactacc cgctggattg
    20701 gtaacccgta caagaaaaac gcgctgatcg attacgacac tctgccgtat ggcgaccagg
    20761 taggtaatca gtaattatca gccaggcgct atttgccgga tgcgacgctt gacgcgtctt
    20821 atccggccta cagatcgcgg cggtagactg aataaggcgt ttacgccaca tccggcaata
    20881 caactgccgc aatgtttaca tcgaactccc ctcaggccac acttttcagg tggccttttt
    20941 taataatgcc agttactgtt ttcccgtcta catacctaag ggaataacat gtatcacgac
    21001 gtcagctatc tgctctcccg cctgatcaac ggcccgctgt ccctgcgcca gatttacttt
    21061 gccagcagta acggtccggc gcccgatctt gcgtatcagg tcgattttcc acggctggaa
    21121 atagtgctgg aaggtgagtt tgttgatacc ggcgctggag caacgttagt tcccggcgat
    21181 gtgctgtacg ttcccgctgg tggctggaat tttccacaat ggcaagcccc cgctactacc
    21241 tttagcgtgc tgtttggcaa acagcaactc ggcttcagcg tcgtgcaatg ggatggcaaa
    21301 caatatcaaa atctggcgaa gcaacacgtc gcccggcgcg gcccacgcat aggttctttt
    21361 ctgctacaaa cgctcaatga aatgcaaatg cagccgcagg agcagcaaac ggcaaggctt
    21421 atcgtcgcca gcctgcttag ccactgccgc gatttgctcg gcagccaaat ccagaccgcc
    21481 tcgcgcagcc aggctctatt cgaagctatt cgcgattata tcgacgaacg ctatgcctcc
    21541 gcgcttaccc gcgaatctgt cgcacaggca ttctatattt cgccaaatta cctctcgcac
    21601 ctgtttcaaa aaacgggggc cattggtttt aacgaatacc tgaatcacac gcgactggag
    21661 cacgctaaga cgttattgaa aggctatgac ctgaaggtaa aagaagtggc gcacgcctgc
    21721 ggctttgtcg acagcaacta cttctgccgt ctgtttcgta aaaacaccga acgctcgccg
    21781 tcggaatatc gtcggcagta ccacagccaa ctgacagaaa aaccgactac tccagaataa
    21841 gatgggtttg ctgcggcgca gaaagcactt tgcgcaccgc gctcattacc cgctgcggct
    21901 cacgcaggaa tgcgtagatg ctgcattgca catagcggca atgttcaaat cgctcggcac
    21961 cggcaagctc gatatccgta atcaacagag caacatccgc ccgacggata tcctcgtccg
    22021 ctaaacgatt ctccgttccc agcgcgccct gagtttcaat gctgacgccc cacttctcta
    22081 actggcacaa cttttccagc cgttccgccg ccatataagt atgcgccacg ccgcttacgc
    22141 aggcggttac tgccaccagg tatgccattt taacctccca cggtaacctg aaatccggcc
    22201 cgttctgcca tttcgcgcat cgttgtcaca tcggctgacg acggcgcagg cacctctttc
    22261 atcgaccatg ttttccccag caggcggtat ttcggttcgc cgtactgatg aaacggtaac
    22321 agatggatct gcctgatttt cagcgggatc agtacatcca gcgcctgctg catattctcc
    22381 cggctgagcg tgaaaccagg gatcaacggt aaacgcggga tcacgttgac accctcactc
    22441 accagcaaac gcagattctc cagcacgcgt ggcaggttca tcttcaccac atcccgcgcc
    22501 tgagtcgcgt ccataatttt taaatcgaac agcacttcat cgcacaattt cgccagtggt
    22561 aacagcttgc tggctggcgc gtctccggca gtttcaatgg cgcatgacac gccccacaac
    22621 cgcagccgct gtaaaaaacg ggtagcaaac tccgcctgca ttaacacttc gccgccagaa
    22681 agcgtgacgc cgccgccgga cgtgcgaaag aaaatgtcat ctttcatcac ttcccgttcc
    22741 agcgcgtcaa ggctgatatc gcgaccaatc cgttcaaacg ccccggaggg gcattcatcc
    22801 gcatcacgca aacatttcgc acagtgcaga catttcgcct ctctgcgtac cgtctggatt
    22861 ttgccggaga tcgactccgg attagcacac catgggcaaa gatgcggaca gcctttaaaa
    22921 aagaccaccg tacgaatgcc ctcgccgtca ttcagtgaat aacgctgaat gttgaaaatg
    22981 cgcgccacat cattgcggcg cgtttccaca acgttacagc tgatgcgctg tccggcggat
    23041 gatgtcatcc tggatctcct tcgacagttc gacaaagaag gcgctgtatc cggcaacgcg
    23101 caccaccagc ccggcataat cttgtgggcg ctgttgcgct tcccgcaacg tgtcggcgtt
    23161 caccacgtta aactgaatat gctgcaactt aagctgggta aacgcccgta agaagtcggc
    23221 cagtttgcgt aatcccgctt caccttccag ggtcgccgga gtgaatttca cgttcagcaa
    23281 cgtaccgtta gacagcagcg tattatcgag cttactgact tcagtaccgc tgttggccct
    23341 tgtgcgtcct ggcccagcat aggtgacaag ccgccgtctg ccagttgttc cccggcaaaa
    23401 cgaccgtctg gcgtcgcgcc aaccaccgat cccaacggga cgtgagcaga aacggtatac
    23461 gatcccggcg tgaagtaacc accgcgcggg ttctggtatt tttccacttc tttgcagtag
    23521 tggcgtaaca gttcggcgct gatgttatcc acctcgtcga tatcgttacc gtatttctcg
    23581 aagcggttaa ttaagcgagc acggactttt tcgccttctg gcgttgcaaa gttggctttt
    23641 aataccgaca gcaattcgtc aaaacttaaa cgctgttgct caaaaaccat ccctttcaac
    23701 gcatggagag aatcgctcag gttggcgata ccgatcccct gtacgccgga gaagttataa
    23761 cgcgcgccgc catcggtaat atcgcgacct ttttccagac aatcgctgat aaaagacgag
    23821 agcagcggta caggtgccca gtcgcgatgg ccgatatcac aaatattgct gccctcaacc
    23881 atcagggtga tgtagtggct gatcttggcg cggatctgct ccagtaaacc ttcataagtc
    23941 agcgcagcat tgccttcatt ttcatgcagg cagatttcca tcactttcag cagattaaac
    24001 atcgcgatgt catgcaagcc gtaggttctg ccgggaatag ataattccac acagcccact
    24061 acggaatagt cgcgcgcatc ttccagcgaa acgccacggt tgaggaacgc tggtaccacc
    24121 acttcatcgt taaagatttg cggaataccg gtgccgaggc gaatggtttc ggcggttttc
    24181 atcaggaacg gcgtgtcgat caaggcgtta gtgcgcacgc cgaggttcgg ttgcggtaat
    24241 tgcacgcttt gataggcgtc aaggcaaagg aacgaaagca cgttcaccgc gctacgtccg
    24301 ttctcggtta acccgccgag cagtgcggta tagccggtcg ggaaacctgc gaaataacgc
    24361 gcgctgctgg tggagcgcaa cagcacgatg tcgttgcatt tcacccataa agattcgagc
    24421 agttctttca ggaacgccgg atcttcgccc tgggttaaag aagcctgata gaacggcaac
    24481 atatactggt cgaagcgccc taaagacagc gaactggcgt tggattcgta ttgcagaatg
    24541 atgttcatgt accagaataa ctggcacgcc tgccagaacg tctgcggctt atgttcggcg
    24601 ttatggcggg agatctccgc aatagtcagc agttcttcgc gacgctgggc atctgtgcag
    24661 tttgccgcca ttgtttccgc cagttcagcg taacgcagaa tgtgtttctg cgaggcttcc
    24721 agcagtaaca gcgcggcctg ataaaagtga ttctccggct gttgctgaca atgttgctgc
    24781 atctgcgcca ccagctcccc cagcccgtga ttcagcaggc gtgggtaatc aataataatg
    24841 tgcccctggc ctttatccgt ctggttaatg ctgaaaatct gcgtgctggt cgcggctttt
    24901 acttcatctg tcatctgccc gttgatgaaa tctttcatcg aacgtttttc ccagtacggg
    24961 aacaactctt cgcggtagat acgtttatct tcttcgctga tagcaaagcg gtcctgcgga
    25021 cgcgtcggga attgatccag ctctttcagc agccagtaag ggtccatttc cggcgacata
    25081 atcccggcgc gcggttttac ggtgcggtta ccggcaatca gttcttcatc acgaatcgaa
    25141 atttcaacat gttcaaggat atacgctgtt gctttcgccc ggcgcaatat caccggttcg
    25201 ccttcggttt gccgatggct ggcggtataa agcagcgccc gctccagcga gatttcacgg
    25261 gtattggaaa acagtgcagt tttgaggcga gagatacgat tcgtcatgag gaactcctgt
    25321 gggaaagcga acccgccctt gcgagcggga gaagaactta agcggtttgc gccaggtgag
    25381 cttcaatttt gctcataatg gcgtcagcac gttttaccgc atcgctgatg ttgacgcgca
    25441 cgatggtctt gccagcaaaa cgctcttcaa acttgatgcc gatatctttg gtcagaatca
    25501 ccatatcggc gctcgccacg tcttccgcag tcagttcgtt ttccagacca attgacccct
    25561 gagtttctac tttcacttcc cagcctttcg ctttcgcggc gctttccagc gcctctgccg
    25621 ccatataggt atgggcaaca ccagatggac acgcggttac tgcaataatt ttcgtcatgg
    25681 ctcgttcctc aattaattaa tttcaaaatc caaatccagg tcgtcttcct tctcatcagt
    25741 cgaagaccca tttttacgcg ccagactttt cagcacgttc acgcaaacag ccgtcaccac
    25801 tgctccgaca gccactgctg cgatataacc cagcttgcct tcaaccaccg gcagcacaat
    25861 cagaccaccc cagcctgcat agcactgcgc accgaccagc gccgcagtta ccgcaccaca
    25921 aacagaaccg accatgatgg aaggaatgac acgcagcgga tcggcagcgg cgaaaggaat
    25981 cgccccttca gtaaccccaa cgcaccccat caccagcgcc gctttgccgg tttcgcgctc
    26041 ttctgcggag aaatttttac gaccaatcag cgtcgccagc cccatcccca gcggcgggat
    26101 acaaataccc acggcagcaa tggccaccac ggtataaaca ccctgagcaa cgcaaatcag
    26161 catgaaggca taggccactt tgttaaccgg accgcccata tcgaacgcca gcatcagccc
    26221 catgatcacc gccagcataa caatgctgcc ctgctgcatc ccctgaagcc actgagtcag
    26281 gctgttggtc aacgccccta caggctcgcc caagccccac atcatgatgc ctgcggtaat
    26341 cagtgtgccg atgataggaa taatgaagat gggcatcacc gagcgcagaa ctttatgcac
    26401 cggaattttc ttcaggtaat gcaccacgat gccgccgata atcccggcga tcagcgcacc
    26461 aaagaagccc gcaccaaagc tgttaccaac ccaggcaccg atagcgcacg gggccagcgc
    26521 agaacgctct gcaatggagt aaccgatgta agcggcgagg aaaggcacca tcagcgtcaa
    26581 acctgcaacg ccgatatcaa acagtttttt cagattcgga tcggctacgg catccggcac
    26641 tgcgcctttg ccatacaaca tgacggaaac cgccagcaaa ataccgcccg ataccacgaa
    26701 gggaatcatg tgtgaaacgc ccgtcattaa atgctgacgg gtatttttca ggatctgcac
    26761 caactcattc ataagctgct cccgggcttg cgaaagccca tgtccatatt tttgatggag
    26821 gaaacaatag gcaatcgcag gcggagcaaa cagtggataa aaaggccatt tactggactt
    26881 ttgtaatatc agtacaaaaa tgcgatccgc ctcataactt gcgataaacc ggaagcatgc
    26941 cgcaaaacag cgtcttgtga gacgcgtcgc aaattgacgg ttacattaca tttgtccagc
    27001 ttttggcaga tttgtcacat ggcaggccgt tgccgtgcgc tcttatcctg taaacagaaa
    27061 accgtaccgg gagagaggca atggccctga ttgtggaatt tatttgtgag ctacctaacg
    27121 gcgtacatgc gcgtccggca agccacgttg agacgctgtg taatactttt tcatcacaaa
    27181 ttgagtggca taacctgcgc actgaccgca agggcaacgc caaaagcgcc cttgcgctga
    27241 ttggcaccga tacgctggcg ggcgataact gccagttact gatttccggg gccgacgaac
    27301 aggaagcgca ccagcgttta agccaatggc tgcgcgatga attcccccac tgcgatgcgc
    27361 cgctggcgga agttaaatct gacgaactgg aaccactgcc ggtttcactg accaatctga
    27421 atccgcaaat tatccgcgcc cgcaccgtgt gcagcggtag tgcaggcggc attctgacgc
    27481 cgatctcttc tttagatctc aatgcgctgg gtaatcttcc cgcagccaaa ggcgttgacg
    27541 ccgagcaatc cgcactggaa aacggcctga cgctggtact gaaaaacatt gagtttcgtc
    27601 tgctggatag cgacggtgcc accagcgcga ttctggaagc gcaccgatcc ctggctggcg
    27661 atacttccct gcgcgaacat ttactggcag gcgtcagcgc cggattaagc tgcgccgaag
    27721 cgattgtcgc cagtgcgaat cacttttgtg aagagttttc ccgttccagc agcagctacc
    27781 tgcaagaacg tgccctggac gtacgcgatg tctgcttcca gttactccag caaatctatg
    27841 gtgagcaacg cttcccggca ccgggcaaac tgacgcagtc cgccatttgt atggctgatg
    27901 aactgacccc cagccagttc ctcgaactgg ataaaaatca cctcaaagga ttgttactga
    27961 aaagcggcgg caccacctca catacggtga tccttgcccg ttcgttcaac attccgacgc
    28021 tggttggtgt ggatattgat gccctcactc cgtggcagca tcaaacgatt tatatcgacg
    28081 gcaacgctgg ggcgattgtg gttgagccag gagaagccgt agctcgttat tatcagcaag
    28141 aagcccgcgt acaggacgcc ctgcgtgagc aacagcgtgt ctggctgacc caacaagccc
    28201 gtaccgctga cggtatccgc attgaaattg ccgctaacat cgctcactcc gtggaagcgc
    28261 aggccgcatt cggcaatggt gcggaaggcg ttggtttgtt ccgcactgaa atgctctata
    28321 tggatcgcac cagtgcaccg ggcgaaagcg agctgtacaa cattttttgt caggcgctgg
    28381 agtctgccaa cggacgcagc attattgtgc gcactatgga cattggcggc gacaaacccg
    28441 ttgattatct gaacattccc gcagaggcaa acccgttcct cggttatcgc gctgtgcgta
    28501 tttatgaaga atacgcctcg ctgttcacca cacaactacg gtcgatcctg cgcgcctccg
    28561 ctcacggcag cctgaaaatc atgatcccga tgatctcttc aatggaagag atcttatggg
    28621 tgaaagaaaa actggcggaa gccaaacagc aactgcgtaa cgaacacatt ccgtttgatg
    28681 agaagatcca gctcggcatt atgctggaag tgccatcggt gatgttcatc atcgatcaat
    28741 gctgcgaaga gattgatttc tttagtattg gtagtaatga cctgacgcaa tatctgctgg
    28801 cagtggatcg cgataacgct aaggttactc gtcactacaa cagcctgaat ccggcattct
    28861 tgcgggcgct cgattacgcc gtgcaggcgg tgcatcgcca gggtaaatgg attggtctgt
    28921 gcggtgagct gggagcgaaa ggttccgtgc tgccgttgct ggtcggctta gggctggatg
    28981 aactcagcat gagcgcacca tcaattccgg cggcgaaagc gcggatggcg caacttgata
    29041 gccgtgagtg ccgcaagttg ctcaaccagg caatggcctg ccgtacttcg ctggaagtgg
    29101 aacacctgct ggcgcaattc cgcatgaccc aacaggacgc accgctggtc accgccgagt
    29161 gcatcacgct ggaaagtgac tggcgcagca aagaagaagt gctcaaaggc atgaccgata
    29221 acctgctgct ggcgggccgc tgccgctatc cgcgtaaact ggaagccgac ttatgggcgc
    29281 gcgaggccgt tttctctacc ggtctgggct ttagttttgc cattccacac agcaaatcag
    29341 aacacattga gcaatccacc atcagcgtgg cgcgtctgca agcgccggtg cgctggggcg
    29401 atgatgaagc gcaattcatc attatgttaa ccctgaacaa acacgctgcg ggcgatcagc
    29461 acatgcgcat tttctcgcgc ctcgctcgcc gcatcatgca cgaagaattc cgtaacgcgc
    29521 tggttaacgc cgcctctgcc gacgctatcg ccagcctgct gcaacatgaa ctggaactgt
    29581 aaaaggaaac atcatggaac tgtatctgga caccgctaac gtcgcagaag tcgaacgtct
    29641 ggcacgcata ttccccattg ccggggtgac aactaacccg agcattatcg ctgccagcaa
    29701 ggagtccata tgggaagttc tgccgcgtct gcaaaaagcg attggtgatg agggcattct
    29761 gtttgctcag actatgagtc gtgacgcgca gggtatggtg gaagaagcga agcgcctgcg
    29821 cgacgccatt ccgggcattg tggtgaaaat cccggtgact tccgaaggtc tggccgccat
    29881 taagatgctg aaaaaagagg gtattactac gcttggcacc gcagtgtaca gtgctgcaca
    29941 agggttactg gccgcgctgg caggggcaaa atacgttgct ccgtatgtta accgcgtaga
    30001 tgcccagggc ggggacggca ttcgtacggt tcaggagctg caagcgctgt tagaaatgca
    30061 cgcaccagaa agcatggtgc tggcggccag ctttaaaacg ccgcgtcagg cgctggactg
    30121 tttactggca ggatgtgaat ccatcaccct gcccttagat gtagcgcaac aaatgctcaa
    30181 cacccctgcg gtagagtcag ctatagagaa gttcgaacac gactggaatg ccgcatttgg
    30241 cactactcat ctctaaagga gcaattatgg accgcattat tcaatcaccg ggtaaataca
    30301 tccagggcgc tgatgtgatt aatcgtctgg gcgaatacct gaagccgctg gcagaacgct
    30361 ggttagtggt gggtgacaaa tttgttttag gttttgctca atccactgtc gagaaaagct
    30421 ttaaagatgc tggactggta gtagaaattg cgccgtttgg cggtgaatgt tcgcaaaatg
    30481 agatcgaccg tctgcgtggc atcgcggaga ctgcgcagtg tggcgcaatt ctcggtatcg
    30541 gtggcggaaa aaccctcgat acagccaaag cactggcaca tttcatgggt gttccggtag
    30601 cgatcgcacc gactatcgcc tctaccgatg caccgtgcag cgcattgtct gttatctaca
    30661 ccgatgaggg tgagtttgac cgctatctgc tgttgccaaa taacccgaat atggtcattg
    30721 tcgacaccaa aatcgtcgct ggcgcacctg cacgtctatt agcggcgggt atcggcgatg
    30781 cgctggcaac ctggtttgaa gcgcgtgctt gctctcgtag cggcgcgacc gccatggcgg
    30841 gcggcaagtg cacccaggcg gcgctggcac tggctgaact gtgctacaac accctgctgg
    30901 aagaaggcga aaaagcgatg cttgccgccg aacagcatgt agtaaccccg gcgctggagc
    30961 gcgtgattga agcgaacacc tatttgagcg gcgttggttt tgaaagcggc gggctggcgg
    31021 cggctcacgc ggtgcataac ggcctgaccg ctatcccgga cgcgcatcac tattatcacg
    31081 gtgaaaaagt ggcatttggt acgctgacgc agctggttct ggaaaacgca ccggttgagg
    31141 aaatcgaaac cgtagctgca cttagccatg cggtaggttt gccaataact ctcgctcaac
    31201 tggatattaa agaagatgtc ccggcgaaaa tgcgaattgt ggctgaagcg gcatgtgcag
    31261 aaggtgaaac catccacaac atgcctggcg gcgcgacgcc agatcaggtt tacgccgctc
    31321 tgctggtagc cgaccagtac ggtcagcgtt tcctgcaaga gtgggaataa cctactccaa
    31381 actcccggct tgtccgggag tttgaacgca aaattgcctg atgcgctacg cttatcaggc
    31441 ctacgcaatc tctgcaatat attgaatttg cttgtttttt aggccagata aggcgttcac
    31501 gccgcatccg gcactctgtg ccagtaatct gaaactccca cgttgttcag gagtttttta
    31561 tccgcttctg gacattttct ctacagaaca atcgttatcc cggaaggtcg aatctgtgac
    31621 tcttattttc acggacgaac cagatgaaag cttcactggc gctgctcagc ctgctaacgg
    31681 catttaccag ccactcctta aaatctcccg ccgttccacc aaccgtggta caaatccagg
    31741 ccaataccaa ccttgccatc gcagacggtg ccagacagca aatcggcagc acgctatttt
    31801 acgatccggc gtatatgcag cttacctatc ctggcggtga tgtcccgcaa gaacgcgggg
    31861 tatgttccga tgtggtgatc cgtgcattgc gcagccagaa ggtcgatttg cagaaactgg
    31921 tgcacgaaga tatggcgaag aattttgccg agtacccgca aaaatggcag ttaaagcgcc
    31981 cggacagcaa catcgatcac cgtcgggtgc ctaatctgga aacctggttt acccgccacg
    32041 ataaaacacg ccccatcagc aagaacccca gtgactatca agcgggcgat attgtctcct
    32101 ggcgattaga taacgggctg gcacatattg gggtggtttc agatggcttc gcccgcgacg
    32161 gtacgccgct ggtgatacat aacattggcg caggtgcgca ggaagaagat gtgctgttta
    32221 gctggcggat ggtggggcat tatcgttatt ttgttaaata agcgatcgcc ccacatgaag
    32281 aaaaaattca gatccttttt acactaacgg cttctttttt cttacggctc accagcgcca
    32341 gtgccatcac aatcagcaca ataccgctac cttcaacggc ccccggattc tcgccgagca
    32401 gccaccagga aaagagtacg ccgcaaacgg gaacggccag tgtgcttaag ctggcaatac
    32461 tggcaggcaa gtttttcaat acaaacaacc ataagctcca cgccagtgcc gtcgccagaa
    32521 tcgcactgta ggccagcgcc cagaacacgg tgggctgcca gtcaatttca cgttgcggca
    32581 ccagtaaagc gaccacactc atcaccagcg ccgcatagag catctgccag gatgttaacg
    32641 acaataaatc cacgcgcgga tgacgggcat acagacgttt agcaacaatc gcgctcgccc
    32701 cccaactgac gccggagagg attgccagca tggcactttt catcgaagag aaatcgagtt
    32761 gccacggctg caacaccaaa aataagccga aagcggcaat cagtatcgcg aaatattgcc
    32821 cacgtcgcag gcgttcaccg agaaacaacg cggcgaaaat caccacccag aacggcatgg
    32881 tatagcttag gatcgccacc ttccccgctc cgccgctgac cagcgcccac tgcgccaggc
    32941 caaccatccc gcaggtttgt aacagggcaa tggctaaggt gtatttaaac ggtgtcgggc
    33001 gcattccgcg accacgtaat aaaaggacga tgaataaaac gagagcgccg aaaatgcagc
    33061 gtaaggcggt aaagtcgaag gcaccgatgt agctggtgac ttgcttcatg aaaatccagc
    33121 tataactcca gataagtgtg agcacaacca ggccactgat tgccagtggg ttgctctttc
    33181 ctgcggcaga catgagagat ccagtgtgta gttttcctgt ggacactata cgccggtaaa
    33241 gaaacttcag cgactgcctg aaaggcagtc gctgaagaag gtcagattac agcaggtcga
    33301 aacggtcgag gttcatcact ttcacccatg ccgccacgaa gtctttaaca aacttctcgt
    33361 gagcatcgct gctggcgtaa acttcagcca ccgcacgcag gacagagtta gaaccaaata
    33421 ccagatccgc acggctggcc gtaaatttca cttcgccggt ttcacggtca cggccttcga
    33481 acagctcttt cgattcgtcg gtcgctttcc actcgtaacg catatccagc aagttcacga
    33541 agaagtcatt gctcaatacg ccaacgcggt cagtgaagac gccgtttttg ctgccatcga
    33601 agttggcacc cagtacacgc atgccgccca ccagcgcagt catttccggc gcggtcagcg
    33661 tcagttgctg tgctttgtcg atcagcagtg actcggtggt ggaaacgtcc agacgagcgc
    33721 gatagttacg gaaaccgtca gcaattggct ccagcagctc aaacatctca atgtcagtct
    33781 gatcctgacg cgcatcaacg cgacccggcg caaacggtac atgaatgctc aaacctgcgg
    33841 cgcttgcggc tttctcaaca ccaaccacac cagccagcac gatgatatcc gccagcgagg
    33901 ctttaccaga ctctttctgg attttctcca gaacaggcag agcacgaacg gctgcggcgt
    33961 tcacatccca gtcgcgctgc ggcattaatg ccagacgcgc accgttggca ccaccgcgtt
    34021 tgtcgccacc acggaaggta gaagcagatg cccaggccac cgataccagc tcactaacag
    34081 acagaccaga atccgcaatc gcgaatttca gatcgataat gtcctgctcg gtcgggttgt
    34141 agatcggctg cggcagcgga tcttgccaga tcagatcttc tttcggtact tccggtccga
    34201 tgtaacgaga tttcggcccc atatccctgt gcgtcagttt gaaccaggca cgggcaaagg
    34261 cttcgttgaa cgcctgcgga tcgttgagga aacgacgaga gatcttctcg aactcaggat
    34321 caaaacgcag cgtcaggtcg gtcaccagca tcgtcggttt acgtttcttc gacggatcga
    34381 acggatccgg gataatttcc ggtgcgtcta ccgcttcgaa ctggattgcg ccagccggac
    34441 tgcgggtctg tacccactca tatttgaaca ggttctcgaa gaaatagttg ctccactggg
    34501 tcggcgtctg ggtccagacc acttccagac cagaggtaat ggcatctgcg ccaacgccgc
    34561 tgccgtaagt gctcgcccaa cctaaacctt gttcttcaat cggtgcagct tctggatcag
    34621 gacctacatt tgatgtcgga ccggcaccgt gggttttacc cagcgtatga ccacccgcaa
    34681 tcagcgccac ggtttcttcg tcgttcatgc ccatgttgcc gaaggtcgcg cggatagctg
    34741 ctgccgcaga aagcggttcg ccgctgtggt ccgggccttc cgggttaacg taaatcaggc
    34801 ccatctcggt tgcacccagc ggtgctttcg ccagcgcttc cggatgacgg tgagtcagcc
    34861 aggctttttc atcaccccag ttaacgtcca gatccggttc ccagacgtct tcacgaccgg
    34921 caccaaaacc gaaggtacgg aagccggagt tttccagcgc cacgttaccc gcgaggataa
    34981 acaggtcggc ccaggagatt ttctgaccat atttctgttt gattggccac aacaggcgac
    35041 gcgctttatc gaggcttacg ttatccggcc aggagttcag cggtgcaaaa cgttgctgac
    35101 cacgacccgc gccgccgcgc ccgtcgattg aacggtaagt acccgcaccg tgccaggcca
    35161 tacgaataaa cagaccggcg tagctgcccc agtcggctgg ccaccacggt tgagattctg
    35221 tcagcagggc tttcagatct tttttcagac cgtagtaatc taatttgctg aattctttgt
    35281 ggtagtcaaa gtcctcaccc agtgggttag aacgattaga atgttggttt aacaggtcaa
    35341 cacggagttg atttggccac cagtcgcgag tggttgtgcc cgcccccgcg ctctggtcgt
    35401 gaccgccctg atggaacggg catttgccag tggctgtggt gttatgaata tcgtctgacg
    35461 tgctcatcaa tgtgctcccc tctacagtgt taccgttacg atacacagcg ttagagagaa
    35521 gttataattg aattaatcca cggatgcgat agttgagatc ttacatattg ttggttaaag
    35581 agatgtagat caaattgatc gtaattaagc ttttttgatt atgtccgaat tcggacaaat
    35641 gctttataaa aaagggtttt agctttatat ccttcaagta atgagggaga aaacaaggct
    35701 gacaacctta ttttcccgcc ctcatttcga ggcagcattt tgtgctctgt ttaaaatttg
    35761 tgatcactgt gtgattttca caaaagccac actatttata aaccaggtcg aacacccagc
    35821 gtatggcaaa tcgcgtaact catttcagcg cggttaagcg tatagaagtg gaaatctttc
    35881 actccttcac ggcttaaaat cttcaccata tccatggcaa tattcgcgcc caccagtttg
    35941 cgggtttcgg catcgtcatc cagcccgtca aacatttgcg ccatccacgc cggaatacgc
    36001 acgttggtca tgtcggcaaa tttcttcgcc tgtttaaagt tagataccgg caaaatgccc
    36061 gggatgattt ccacatcaat gcccgccgat acacagcggt cacgaaaacg caggtagctt
    36121 tcgacatcga agaagaactg agtaatcgcg cggttggctc cggcatccac tttgcgtttc
    36181 agattaagca aatccgcctg agcgcttttt gcttccgggt gaacttccgg atacgccgcc
    36241 acggatatat cgaaatctgc cacttctttt aacagcgtca ccaggtcaga agcatacatt
    36301 tctggcttac cacttcccgg cggcagatcg ccacgcagcg ccacaatatg acgaataccg
    36361 ttattccagt agtcgcgtgc aatggtgcgc agctcgtcgg gcgtcgcatc aatgcaagta
    36421 agatgcggtg ccgcttccag accagtgcga tctttaatgc ctttaataat gctgtgcgta
    36481 cggtcgcgct cgccggagtt cgcgccatag gtcaccgata caaacttcgg tttcaggctg
    36541 ctaaggcgat cgatggagtt ccacagggtc tgctccattt cactggtacg cggcgggaaa
    36601 aactcgaacg aaacgttaat ttgcccctgg acttctgcca ggctctgatt cagggcatcc
    36661 cgctggctgg cgtgaaaaaa gctcatacct tacctcatca atcgcttgta gttatatgtt
    36721 gtgttgtgca catctatccg tttagacgtc cagatgtaaa gatgaaggaa aagccgaagg
    36781 gcgtcaaccg aaaaatgacc aatgatggtg aggaaaaatc agggaagatg aaaaaacttc
    36841 aggatggtaa gaaaaagaaa atccggtatg caaagcaaac cggactgcaa atataaacta
    36901 accatgtact tattgcttct tgccggattc gtcgtaaacg cttaatccgg cctaatccta
    36961 tgataattca atatatttca ttagaaacca taaacccgaa aacatgagta ccgggcatta
    37021 ttaaatttct gaaattacaa caactgtgcc agccggttga tatccgactg aatcgccccg
    37081 gcggtgacgt cgcgccccgc gccaggccca cggatcacca gcgggttgtc gcgataccag
    37141 cggctttcga tggcaaagac gttatcgcac ggcagcagtg atgccagcgg atggtcttca
    37201 cgcaccgcct ccacgcccac acgcgctttg ccgttggcat cgaaacgcgc cacgtagcgc
    37261 agcaccagcc ccatttcgcg ggccgcttcc agccgttgca ccatctgctc gttaagttca
    37321 tcgccatttt caaagaaatg gtcgatgctg ccgccttcgc aatgagcagg caccagcgat
    37381 tccacacgta cctggtccgg ttcaatgttg taacccgctt cacgcgccag aattaccagc
    37441 ttgcgcatta cgtctttgcc ggagaggtcg tcacgcggat caggctcggt taagccctgc
    37501 tgccacgcct gatccaccag ctcggtaaac ggcacgctac cgtcgaattg caggaacagc
    37561 caggagagcg tgccggagaa gatcccgctg atcgacaaaa tagtatcgcc gctgtcgatc
    37621 agatcgcgca cggtgtggtt gatcggcaag cccgcaccga cggtggcatt gtacagccag
    37681 tgacgcccgg ttttttcgaa ggcgtcgtgg atctggcgat atttattgct gtcgctggct
    37741 cccgccagtt tgttggcgct gataacgtgg aaaccgtggc tggcgaaatc aagatactga
    37801 tcagcaagct gctggctggc ggtaacgtcc agcaccacta aatcatcata cggatgggcg
    37861 cgcatccaca ggaacaacga ctcttcatcc tgctcaaccg cttcatcgtt gaagaaggct
    37921 aacgcgcggc tggcgtccag cccgtcatag ctcaacaggc tgcggcggct gtccaccaca
    37981 cctgccagca caaactcaaa gccggtacgt gccgaaagcg tgctctgctc acgggcgaac
    38041 agttccagcc aacgggaacc gatattgccc ttaccgaaca ataccaggcc gatgcgtttt
    38101 tctgcgcgga agacggactg atgcagcccc tgaatcaggc tttcggtcgg gccggtgcgc
    38161 agtactgcca ccaggctgat gccgtcatcg gactgccagg taaattcgac cggctggcct
    38221 ttcagttgct gccagaagcg gtggcaatgc agcgggttac gggtgacgcc tgcaccgacc
    38281 atcgccacca gcgccagccc ctgacgcagg cgcagttcgc caggtaatcc cgcttcgtcg
    38341 aggattttca gcgcactgtc ggccacttct gaggtgtagc aaaattgcag caactggcga
    38401 tcgttatgta cgccaaccgc cagcgggcgt acctgcgcgc gtttcaggat ttggtcgatc
    38461 tctttatgcg ccagtttgaa atcctgactg gcgggcacct gaaactcaat caaacagaca
    38521 tcatcgtggc tggtgacaat acgcgcacca gtaccggagg ccagcacgcg ttcaatgcgc
    38581 gtggaacctt gatccggcgt gtagctacag cgcagttgca ggtcgatttc gctgccagaa
    38641 accggctgta aagtacgggc gtgaagaacg ggagccgcca ggcgcgccag ttcgctggcc
    38701 tcatccagac gcagcaacgg cagcaggcag gcatctttca ctttacgcgg gtcggcactg
    38761 tataccccgg cgacgtcgct ccagatggtt acgcgagaaa cacccgccag cgcaccgatt
    38821 tgtgtcgcgg aatagtcgga accgttacgc cccagcagca ccgtttcacc ggcgttgttg
    38881 cggctgataa atccggtcac caccagacgt ttgcccggat gttgcaccag cagctgttgc
    38941 agcaacgggt aagaaagccc ttcatcaacc tgcggttgtg cggcgcgttc agcgcgtaaa
    39001 aactcgcggg catcaagcca ggccgctggc agcccttgtt gattaagtac cgcagacatc
    39061 aggcgtgccg accacacttc accgtgcccc accacttccg catacactgc gtcgttaata
    39121 ccgctgtcga gcagcgccgc caggcgctca aggtcgctga caaaagcgct aatgaggcta
    39181 tcggcttctt cagcgggtag cagaccgcta atcagatcgc actgatagcg acgcagcgtt
    39241 tgttgaacct gatgcgcaga gagacgatcg gtctggctta gtttcaacca gttaatcaac
    39301 tggttagtgg tgctaccggc ggcggaaacc accatcatat cgtcaggctg agagtactcc
    39361 gccataatgc ccgcgacacg caaataacac ttcacatcag ccagactact gccaccaaat
    39421 ttatgcagct gacgaccttt cgcccctgcc tgcgcaatca cactcatttt taccccttgt
    39481 ttgcagcccg gaagccattt tccaggtcgg caattaaatc ttcgccatct tcaataccgg
    39541 tggagatacg cagcagcgtc tcggagatcc cggcggcagc acgcgcttct ggtgccatgc
    39601 ctgcatgtgt catggttgcg gcgtgagaga ttaaactttc cactccccct aatgattccg
    39661 ccagcgtaaa caacgacagc ccgctcagga aacgacgcag cgtctgctca tcgccatcca
    39721 gttcaaaact caacattgcg ccaaagcctt tttgctgacg cgcggcaatt tcatgcccct
    39781 gattttccgg cagcgacggg tgatacagtt ttttcaccaa cggctgggtt tgcagatatt
    39841 tcacaatcgc ctgcgcgtta cgctgggcca actccatgcg cggcaccagc gttcgcaacc
    39901 cgcgtagcag cagatagctg tcaaacgcgc cgcccgtcac gccaatattg tttgcccacc
    39961 aggcaagttc agtgacaacg tccgggtctt tagcaatcac cacgccggcc accacgtctg
    40021 agtgaccgtt cagatatttc gtgcatgaat gcaacaccag atcggcacct aatgccagcg
    40081 gattttgtaa tgccgggctt aagaaggtgt tatccaccac gctcaccgcc ccgacttccc
    40141 ttgccagatg gcagattttc gcaatatcca cgacgcgtaa caatggatta cttgggcttt
    40201 ctaccagtac cagtttgggt ttttctgcca gcgctgcccg taatgcctgt tcatcgcctt
    40261 gatcaacaaa caacacgcga tagcaaccac gtttcgccag gctgtcgaac aggcgatagc
    40321 taccgccgta gcagtcgtgc ggcgctacca gcagatcgcc aggtttcaaa aagacggtcg
    40381 ttaccaggtg aatcgcggac atgccggtat tagtaagtac tgcaccagca ccgccttcca
    40441 gttctgccag tgctcgctga accacatcgc gcgttgggtt gccacgacgc gagtaatcat
    40501 gcgcgcgcgg ttcattaaat ccggtaaagt tataggtact ggaaagatgg atgggtggga
    40561 caacgcaacc atactgttcg tcgtcattta acccgctacg cactgcgatg gtggcctgtt
    40621 tacgcgtcat gtgatgatgt tccctgggct ttattggtga aatgtcaggc accagagtaa
    40681 acattgtggt aatggacgtc aatacatctg gacatctaaa cttctttgcg tatagattga
    40741 gcaaatccca aatagccgtt aaaattatat gcattatcac gccagcaggt ggattacccg
    40801 atgtcacggt agcgcctgta cggtaaacta tgcgggttta cggccagtgc ccacattaac
    40861 tgtgtggtct ggtctcaatt tattgacgaa gaggattaag tatctcatgg ctgaatggag
    40921 cggcgaatat atcagcccat acgctgagca cggcaagaag agtgaacaag tcaaaaagat
    40981 tacggtttcc attcctctta aggtgttaaa aatcctcacc gatgaacgca cgcgtcgtca
    41041 ggtgaacaac ctgcgtcacg ctaccaacag cgagctgctg tgcgaagcgt ttctgcatgc
    41101 ctttaccggg caacctttgc cggatgatgc cgatctgcgt aaagagcgca gcgacgaaat
    41161 cccggaagcg gcaaaagaga tcatgcgtga gatggggatt aacccggaga cgtgggaata
    41221 ctaaagcgga aaagagcggc gcggagtgga accgcctgat gcgctacgct tatcaggcct
    41281 acgtcatatt gcaatttatt gaatttgcac gaacttgtag gccggataag gcgttcacgc
    41341 cgcatccggc ataaacaacg agcacgttgt ctgcgaccca ccgcttttta tacatggacg
    41401 tttaactatg aaaaacaggc tgctgatcct cagcctgctg gtttctgtac ctgcctttgc
    41461 ctggcagccg caaactggcg acatcatctt tcaaatctct cgctcatcgc aaagtaaagc
    41521 gatccaactg gcaacccatt ccgattacag ccacaccggt atgctggtga tacgcaacaa
    41581 aaagccctac gtttttgaag cggtcggccc ggtgaaatac accccgctca agcagtggat
    41641 cgcccatggt gaaaagggca aatacgtcgt tcgccgcgtt gaaggcggac tgagtgttga
    41701 acaacagcaa aaactggcgc aaacggcaaa acgttatctc ggtaaaccgt atgatttcag
    41761 cttctcatgg agcgacgatc gccagtactg ttcggaagtg gtgtggaagg tttaccagaa
    41821 cgcgctggga atgcgcgtgg gcgaacagca gaagttgaaa gagtttgatc tcagcaaccc
    41881 gctggtccag gcgaagctca aagaacgcta cggtaaaaat attccgcttg aggaaacggt
    41941 agtttcacca caagccgttt tcgacgcgcc acaactcacc acggttgcca aagagtggcc
    42001 gctgttttcg tggtaatgga tgatgaaaaa aataattccc gccgtagtct gtttgttgct
    42061 ttgtagttgc acgacctttc cgcaagctgt cgctccggta aacggtgatg cctccaatta
    42121 gttgaattaa ggatataata ctcgctttta aggttgtttc atggtcacag ttaaccttca
    42181 ttgcccccgt tgccagtccg ttcaggttta ccgtcacggg cagaacccaa aaagacatga
    42241 tcgattccgc ggccgtgact gtcaccgggt atttcagctc agttactgtt acgagccccg
    42301 taaaccggga gttaaagacc aaatcgctga aatgactttc aacggtgccg gggttcgcga
    42361 tactgcccgt acgctgaaaa tcggcattaa tacagtcatc cgaactttaa aaaactctcg
    42421 ccacgaagaa taacgtcatc cccggtcgct catgctgatg tggcactcat ctgcaaactt
    42481 agtcagcaat ggagctttgt cggcagtaaa gcgcgacagc actggctctg gtacgtgtac
    42541 aacacgaaaa ctggtggtgt tctggcctac acttttggcc cccggactga cgaaacctgt
    42601 cgtgaattgc gggcgctgct taccctttta ccatcggcat gttgaccact gacgaatggg
    42661 gtagttacgc tagagagtta ccgaaggaga agcatctgac tggtaagatt tttactcagc
    42721 acattgagcg taacaatctg accctgagaa cccgaatcaa acgtctggcc cgcagaacca
    42781 tctgtttctc gcgttctgta gaacttcatg aaaaagtcat cggtgctttt atcgaaaata
    42841 tatgttctac taattggagt catcaccctt ctgataagct ttccttggat gattaccaga
    42901 ctgaatacac agccaaagat ttttacctca ttctgctctt ttttctcaaa tgacatatat
    42961 tatattttac atcatattca taggcatatg acattccccc gccccccatt atgtcattgt
    43021 gggttgatgt tgggctttca taaaaaaaaa catctttttt tgaactagca ttaattatat
    43081 ttactttata tattaaaata
//
LOCUS       DAZGSP010000037        42300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZGSP010000037 DAZGSP010000000
VERSION     DAZGSP010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.037"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(56..337)
                     /gene="yehE"
                     /locus_tag="ACHK74_002129"
     CDS             complement(56..337)
                     /gene="yehE"
                     /locus_tag="ACHK74_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFP0220949.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRYLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(600..1709)
                     /gene="apbC"
                     /locus_tag="ACHK74_002130"
     CDS             complement(600..1709)
                     /gene="apbC"
                     /locus_tag="ACHK74_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFP0220950.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INSVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1841..3874
                     /gene="metG"
                     /locus_tag="ACHK74_002131"
     CDS             1841..3874
                     /gene="metG"
                     /locus_tag="ACHK74_002131"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFP0220951.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4015..7809
                     /locus_tag="ACHK74_002132"
                     /pseudo
     CDS             4015..7809
                     /locus_tag="ACHK74_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7819..11451
                     /gene="yehI"
                     /locus_tag="ACHK74_002133"
     CDS             7819..11451
                     /gene="yehI"
                     /locus_tag="ACHK74_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFP0220952.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPAQTMAALVCIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFITRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIATLPGITKVRRPFIALILPEKPEIANELVSL
                     ECPRTHFHSKEWLKVVANDPTAVRKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLIASQPQLAER
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHTLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIIPPLRQLTRRTVLLTPDESASNSLNRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYSDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11512..11832
                     /gene="yehK"
                     /locus_tag="ACHK74_002134"
     CDS             11512..11832
                     /gene="yehK"
                     /locus_tag="ACHK74_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFP0220953.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     LIEY"
     gene            13015..14103
                     /gene="yehL"
                     /locus_tag="ACHK74_002135"
     CDS             13015..14103
                     /gene="yehL"
                     /locus_tag="ACHK74_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFP0220954.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            14114..16392
                     /gene="yehM"
                     /locus_tag="ACHK74_002136"
                     /pseudo
     CDS             14114..16392
                     /gene="yehM"
                     /locus_tag="ACHK74_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            16385..17521
                     /gene="yehP"
                     /locus_tag="ACHK74_002137"
     CDS             16385..17521
                     /gene="yehP"
                     /locus_tag="ACHK74_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP0220955.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDHDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            17518..19518
                     /gene="yehQ"
                     /locus_tag="ACHK74_002138"
     CDS             17518..19518
                     /gene="yehQ"
                     /locus_tag="ACHK74_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFP0220956.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCTTAQPTEKEE
                     AWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFANDEG
                     NACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            19643..20104
                     /gene="yehR"
                     /locus_tag="ACHK74_002139"
     CDS             19643..20104
                     /gene="yehR"
                     /locus_tag="ACHK74_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFP0220957.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(20145..20615)
                     /gene="yehS"
                     /locus_tag="ACHK74_002140"
     CDS             complement(20145..20615)
                     /gene="yehS"
                     /locus_tag="ACHK74_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFP0220958.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(20662..21381)
                     /gene="btsR"
                     /locus_tag="ACHK74_002141"
                     /gene_synonym="yehT"
     CDS             complement(20662..21381)
                     /gene="btsR"
                     /locus_tag="ACHK74_002141"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFP0220959.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(21378..23063)
                     /gene="btsS"
                     /locus_tag="ACHK74_002142"
     CDS             complement(21378..23063)
                     /gene="btsS"
                     /locus_tag="ACHK74_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFP0220960.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            23285..24016
                     /gene="mlrA"
                     /locus_tag="ACHK74_002143"
     CDS             23285..24016
                     /gene="mlrA"
                     /locus_tag="ACHK74_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFP0220961.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            24076..24183
                     /gene="yohO"
                     /locus_tag="ACHK74_002144"
     CDS             24076..24183
                     /gene="yohO"
                     /locus_tag="ACHK74_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFP0220962.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(24164..24895)
                     /gene="yehW"
                     /locus_tag="ACHK74_002145"
     CDS             complement(24164..24895)
                     /gene="yehW"
                     /locus_tag="ACHK74_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFP0220963.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(24900..25826)
                     /gene="yehX"
                     /locus_tag="ACHK74_002146"
     CDS             complement(24900..25826)
                     /gene="yehX"
                     /locus_tag="ACHK74_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFP0220964.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVMNTQGQPCGTLHFQDLLEEA"
     gene            complement(25819..26976)
                     /gene="yehY"
                     /locus_tag="ACHK74_002147"
     CDS             complement(25819..26976)
                     /gene="yehY"
                     /locus_tag="ACHK74_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFP0220965.1"
                     /translation="MTYLRINPVLALLLLLTVIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(26983..27900)
                     /gene="osmF"
                     /locus_tag="ACHK74_002148"
     CDS             complement(26983..27900)
                     /gene="osmF"
                     /locus_tag="ACHK74_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFP0220966.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(28111..30408)
                     /gene="bglX"
                     /locus_tag="ACHK74_002149"
     CDS             complement(28111..30408)
                     /gene="bglX"
                     /locus_tag="ACHK74_002149"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFP0220967.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            30604..32319
                     /gene="dld"
                     /locus_tag="ACHK74_002150"
     CDS             30604..32319
                     /gene="dld"
                     /locus_tag="ACHK74_002150"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP0220968.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(32357..33289)
                     /gene="pbpG"
                     /locus_tag="ACHK74_002151"
     CDS             complement(32357..33289)
                     /gene="pbpG"
                     /locus_tag="ACHK74_002151"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFP0220969.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(33463..34050)
                     /gene="yohC"
                     /locus_tag="ACHK74_002152"
     CDS             complement(33463..34050)
                     /gene="yohC"
                     /locus_tag="ACHK74_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFP0220970.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            34220..34798
                     /gene="yohD"
                     /locus_tag="ACHK74_002153"
     CDS             34220..34798
                     /gene="yohD"
                     /locus_tag="ACHK74_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP0220971.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(34928..35689)
                     /gene="yohF"
                     /locus_tag="ACHK74_002154"
     CDS             complement(34928..35689)
                     /gene="yohF"
                     /locus_tag="ACHK74_002154"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0220972.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(35742..37178)
                     /gene="mdtQ"
                     /locus_tag="ACHK74_002155"
     CDS             complement(35742..37178)
                     /gene="mdtQ"
                     /locus_tag="ACHK74_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFP0220973.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHFDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            37402..37485
                     /gene="yohP"
                     /locus_tag="ACHK74_002156"
     CDS             37402..37485
                     /gene="yohP"
                     /locus_tag="ACHK74_002156"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFP0220974.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(37873..38823)
                     /gene="dusC"
                     /locus_tag="ACHK74_002157"
     CDS             complement(37873..38823)
                     /gene="dusC"
                     /locus_tag="ACHK74_002157"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFP0220975.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKKYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            39062..39460
                     /gene="yohJ"
                     /locus_tag="ACHK74_002158"
     CDS             39062..39460
                     /gene="yohJ"
                     /locus_tag="ACHK74_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFP0220976.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            39457..40152
                     /gene="yohK"
                     /locus_tag="ACHK74_002159"
     CDS             39457..40152
                     /gene="yohK"
                     /locus_tag="ACHK74_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFP0220977.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            40282..41166
                     /gene="cdd"
                     /locus_tag="ACHK74_002160"
     CDS             40282..41166
                     /gene="cdd"
                     /locus_tag="ACHK74_002160"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFP0220978.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            41316..42035
                     /gene="sanA"
                     /locus_tag="ACHK74_002161"
     CDS             41316..42035
                     /gene="sanA"
                     /locus_tag="ACHK74_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFP0220979.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            42038..42277
                     /gene="yeiS"
                     /locus_tag="ACHK74_002162"
     CDS             42038..42277
                     /gene="yeiS"
                     /locus_tag="ACHK74_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFP0220980.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
BASE COUNT        10297 a        11198 c        10666 g        10139 t
ORIGIN      
        1 tcatttatat aaatgatttc ggctttctaa atgcaattaa aatcaggttg ccaaattaat
       61 cgtagcgatt taacacgatt cgacgtttgc tatcgctacc tgcaacaacc acttctgtcg
      121 ttacaccttt gacgggcgaa gaaaacagat aacgggtgtc aacattatac gttaactgac
      181 cacaatgctg ttgaggttga ccattaacca tagccatact gcatgttggg ggactgatcc
      241 tgccattaat cgctaatgtg gcagtctctg aagagaatgc gggagaagcc aatccatagg
      301 caagaaaaat aattcctgac agccaatact tattcattga acgttatccc tgtagtaaag
      361 gttatgcctg gatagaatga gtgcataaca aactatagct gtacatccac tacacagcca
      421 cgaaggatga taatgaagca ttgcttgtat gatcaatcga ctttgtagag tttcggacga
      481 aggtccgcag aatattcgca gtattaaata agtgttcatt aatgcggcaa aaactaatac
      541 accgcatcaa tgtaacatct ctgcggtgta tgaagaataa ccagaatggt taataggcgt
      601 tagaccgcgc ggaaggaaat ctcgcctgga atgacttcac cctgccagta gagctgagct
      661 gcaacgcggt cagccagctg gcggtagata gcggtaaatt cgctctccgg acggctgata
      721 accgttggcg tgcctttatc gagatcctca cgtaaggaga tgtgtaacgg catctgcccc
      781 agcagctgtg tgtggtattt ttcagccagt ttctcagcac caccggtacc gaaaatcggt
      841 tcatggtgac cacagttact gcaaatatgc acactcatgt tctcgacaat accaagcacc
      901 ggaacctcga ctttctcgaa catcacaatg ccttttttcg catcgatcaa cgcgatgtcc
      961 tgcggggtgg tgaccacgac tgcaccagtt actggaatgt tttgcgccag cgtcaattga
     1021 atgtcaccgg tgcctggcgg catatcaagc accagatagt cgagatccgg ccacaaggtt
     1081 tcctgcaaca tctgcattaa cgccttgctc gccatcggtc cacgccacac catcgcattg
     1141 tcgtcagtga ccagataacc aattgaattg gttgccaggc catgagacat aatcggtgcc
     1201 atgtgggtac cgtccggcga cgttggacgc tgattttctg cgcccagcat ggtgggaatt
     1261 gatgggccgt agatatcggc gtccagaatg ccgactttcg ccccttccgc cgccagtgcc
     1321 agcgccaggt ttaccgccgt ggaggattta cccacgccgc ctttaccgga gctgaccgca
     1381 atgatgttct tcacactatt aatacccggc tgattcttca cgcgttttaa ggtggcgata
     1441 ttgtgcgaca gtttccagtc gatagctttc gccccggtga tacgcagcag ttcggcgcta
     1501 cattgctctt ttaactcttc aaacgcgcta tgccagacga atggcataac cagttccaca
     1561 tgcaacgtgt cgtccatcca ggcgacatgg tgcaacgctt tcagcgtggt gaggttatgc
     1621 ttcagggttg ggtgctgaaa attggccagt gtcccggcga ccattgcgcg caaggcttct
     1681 ggtgatttgg cctgggattg ttcgttcatc ccgactcctt ttttgtatag ataaaccatc
     1741 agctgatagt ttacctgaag aatatagaga agtacttact taacattttc ccatttggta
     1801 ctatctaatc ccttttcact attaagaagt aatgcctact atgactcaag tcgcgaagaa
     1861 aattctggtg acgtgcgcac tgccgtacgc taacggctca atccacctcg gccatatgct
     1921 ggagcacatc caggctgatg tctgggtccg ttaccagcga atgcgcggcc acgaggtcaa
     1981 cttcatctgc gccgacgatg cccacggtac accgatcatg ctgaaagctc agcagcttgg
     2041 tatcaccccg gagcagatga ttggcgaaat gagtcaggag catcagactg atttcgcagg
     2101 ctttaacatc agctatgaca actatcactc gacgcacagc gaagagaacc gccagttgtc
     2161 agaacttatc tactctcgcc tgaaagagaa cggttttatt aaaaaccgca ccatctctca
     2221 gctgtacgat ccggaaaaag gcatgttcct gccggaccgt tttgtgaaag gcacctgccc
     2281 gaaatgtaaa tccccggatc aatacggcga taactgcgaa gtctgcggcg cgacctacag
     2341 cccgactgaa ttgatcgagc cgaaatcggt ggtttctggc gctacgccgg taatgcgtga
     2401 ttctgaacac ttcttctttg atctgccctc tttcagcgaa atgttgcagg catggacccg
     2461 cagcggtgcg ttgcaggagc aggtggcaaa taaaatgcag gagtggtttg aatccggcct
     2521 gcaacagtgg gatatctccc gcgacgcgcc atactttggt tttgaaattc cgaacgcgcc
     2581 gggcaaatat ttctacgtct ggctggacgc accgattggc tacatgggtt ctttcaagaa
     2641 tctgtgcgac aagcgcggcg acagcgtaag cttcgatgaa tactggaaga aagactccac
     2701 cgccgagctg taccacttca tcggtaaaga tattgtttac ttccacagcc tgttctggcc
     2761 tgccatgctg gaaggcagca acttccgcaa gccgaccaac ctgtttgttc atggctatgt
     2821 gacggtgaac ggcgcaaaga tgtccaagtc tcgcggcacc tttattaaag ccagcacctg
     2881 gctgaatcat tttgacgctg acagcctgcg ttactactac actgcgaaac tctcttcgcg
     2941 cattgatgat atcgatctca acctggagga tttcgttcag cgtgtgaatg ccgatatcgt
     3001 gaacaaagtg gttaacctgg cctctcgtaa tgcgggcttt atcaacaagc gttttgacgg
     3061 cgtgctggca agcgaactgg ctgacccgca gttgtacaaa accttcactg atgccgctga
     3121 agtgattggt gaagcgtggg aaagccgcga atttggtaaa gcagtgcgcg aaatcatggc
     3181 gctggctgat ctggctaacc gctatgtcga cgaacaggct ccgtgggtgg tggcaaaaca
     3241 ggaaggccgc gatgccgacc tgcaggcaat ttgctcaatg ggcataaacc tgttccgcgt
     3301 gctgatgact tacctgaagc cggtactgcc gaaactgacc gagcgcgcag aagcattcct
     3361 caatacagaa ctgacctggg atggtatcca gcaaccgctg ctgggccaca aagtgaatcc
     3421 gttcaaagcg ctgtataacc gcatcgatat gaagcaggtt gaagcactgg tggaagcgtc
     3481 taaagaagaa gtgaaagcag ctgccgcgcc agttactggc ccactggcag atgatccgat
     3541 tcaggaaacc atcacctttg acgacttcgc aaaagttgac ctgcgcgtgg cgctgatcga
     3601 aaacgcagag tttgttgaag gttctgacaa actgctgcgc ctgacactgg atctcggcgg
     3661 tgaaaaacgc aatgtcttct ccggtattcg ttctgcctac ccggatccgc aggctctgat
     3721 tggtcgtcac accattatgg tggctaacct ggcgccgcgt aaaatgcgct ttggtatctc
     3781 cgaaggcatg gtaatggctg ctggtcctgg cgggaaagat atcttcctgc taagcccgga
     3841 tgccggtgct aaaccgggtc atcaggtgaa ataatccccc ttcaaggcgc tgcatcgaca
     3901 gcgccttttc tttataaatt cctaaagttg ttttcttgct attttgtctc tctctaaccc
     3961 gcataaatac tggtagcatc tgcattcaac tggataaaat tacagggatg cagaatgaga
     4021 cactttatct atcaggacga aaaattacat aaattctggg cggttgagca acagggaaac
     4081 gagttgcata tcagttgggg aaaagttggc accaaagggc aaagccagat aaaaagtttt
     4141 tcagatgctg cggcagtggc aaaagcggag cttaagctga ttgcggagaa ggtgaagaag
     4201 gggtatgtgg agcaagcgaa ggataattct ttgcaacctt cccaaacggt aacgggctct
     4261 ctcaaggtag cggacttatc caccattatt caggaacaac cctctttcgt agcagaaacc
     4321 cgtgcgcctg acaaaaatac agatgctgtt ttaccgtggc tggcgaaaga tattgctgtc
     4381 gtttttccgc ccgaagttgt acacaccacg ttaagtcatc gccgctttcc cggagttcct
     4441 gttcagcaag cagacaaatt gacccaatta cgtcgcttag cctgtagtgt gtcgcaacgg
     4501 gataataaaa cagccacatt tgacttcagc gcctgttctt tagaatggca aaacaccgtc
     4561 gcccaggcga tcagtcagat cgacggcctg aaaacaacac agctaccatc accagtaatg
     4621 gctgtactca cggcacttga aatgaaatgc acaagatata aagtgcgtga ggatgttatg
     4681 gatcagatcg tccaggaagg cggtctggaa tatgctactg atgtaataat acaccttcaa
     4741 cagattgata ttaaatggga ttatgcgaat aatgtcatta ttattctgcc gtctggcatt
     4801 gcacctgact acttggagca atattccaga tttgaattac gcctacgtaa acatttatca
     4861 ctggcggaag agtctctctg gcaaaaatgt gcacaaaaac ttattgccgc aattccacat
     4921 attccagaat ggcggcaacc attaattgct ttgttattac ccgaaaaacc agaaattgca
     4981 catgaaattg cccagcgtct actggggcaa aaaaaattac cctcgcttga gtggttaaaa
     5041 atagtggcga ctgatgagca cattcttgcc tcattagaaa aatatcacga gccatatgcc
     5101 atttttgatg attactattg tggtgcgata tggtcagcca ccgtattaca ggagcaaggt
     5161 gttgcagccc tgccccgatt tgctccctat gccgcaagtg actactgcgc cgatgtgttg
     5221 cgtcatatca atcatccgtt cgcattaaca ctgcttatac gtgtagccgg gcatactaaa
     5281 cgctgtcacg atcggatgac gaaagcctgc gctgcgttcc cacatgcagc aatggcggca
     5341 ctgacggaac ttcttgggca aaaagaagag aacagttggc gcattatgct aatgacaatg
     5401 cttatctcac aaccagcact ggcagaacag gtcattccct ggctctcgac acccgcagtt
     5461 gccgtactga aatcatgcca gcaacaactg acacagccct caaaccatgc cagcgccgat
     5521 ctactgccag ccgtagtagt ctcccctccc tggctttcga aaaagaaaaa atcgccgatt
     5581 ccggtgctgg atttagcgcc attaggcatt gagccaatct gttatctgac agaagaaatc
     5641 agtaatcaac ttttggcgaa atatatctgg tattcaaaac acatcacggt tagccatgaa
     5701 gaaagtacta ccaacctgtt ggcaaggatg ggttttcaac gacggatcgc tggtacatat
     5761 attaaagctc ccgaagcggt agttgaggca tggctaaatg aagattattc aaccttacta
     5821 agtgaattta aggtgtttca ttcacctacc gggcattatt ggcagttggg gattttgaca
     5881 acattgccgc tggagaaagc agtaaaagca tggaatgccc ttaccctatc tccacatacc
     5941 gataccgaat acgccatgtt acattttgga ctcaaagggt tacctgggtt agtaaactca
     6001 cttgcacgct atccacaaga agccttgccc atcacgaatt acttcgcagc gagtgagctg
     6061 gctcctgccg tcgcccgtgc cttcaacaaa ctgaaaacgc tacgcgaaaa cgcccgtagc
     6121 tggctgttga aatacccgga acatgccctt accggcctgc tgcctgcggc gctcggcaaa
     6181 gccggtgaag cacaggataa cgcccgcgct gccttgcgta tgcttaccga aaacggtcat
     6241 cagccattac tgcaagaaat cgcccgacgt tataaccagc cggaagtaac cgatgcggtg
     6301 aacgctctgc ttgcgctcga tcccttagat aatcacccga caaaaatccc cactcttccg
     6361 gccttttatc agccatcgct ctggacgcgc ccggtattaa aagcaaacgc ccaatcactg
     6421 ccagataacg ccctcctcca cctcggcgaa atgctccgct tccctcagga agaggctctg
     6481 tatccgggat tattgcagat gaaagatgcc tgtaccgccg actcactggc tgaatttgcc
     6541 tgggatctgt ttaccgcctg gcagaccgct ggcgcgccgt cgaaagagag ttgggcgttc
     6601 actgcgttag gcgttctcgg taacgatgac accgcccgca aactgacgcc attaatacgc
     6661 gcctgacctg gtgaatccca gcataaacgc gccaccgttg ggttggatat tctcgctgct
     6721 atcggtagtg atatcgccct tatgcagctt aacggcatcg cccagaaact gaaattcaaa
     6781 gcattacagg agcgggcaaa agaaaaaatt gccgacattg ccgagagccg cgaactcacg
     6841 gtggcggagc ttgaagatcg gttagcaccg gatctcggtc tggatgataa cggttcgctg
     6901 ctgctggatt ttggcccacg gcagttcacc gtcagctttg atgaaacctt aaaaccgttt
     6961 gtgcgtgatg tttccggcag ccgcctgaaa gacctgccca aaccgaacaa aagcgatgat
     7021 gaaacgcggg cgaacgatgc ggttaaccgc tacaaattgc tgaaaaaaga tgcgcgtacc
     7081 atcgccgccc agcaggtagc aaggctggaa tccgccatgt gcctgcgccg ccgctggtcg
     7141 ctggaaaact tccagctctt cctggttgag catccgctgg ttcgtcactt aacccgccgt
     7201 ctgatttggg gcgtttatag cgccgaaaac cagctactgg cttgctttcg cgtagcagaa
     7261 gataacagct acagcaccgc tgacgatgat cttttcaccc tgccggaagg cgatatctct
     7321 atcggcactc ctcacgttct ggaaatatca ccaacggatg ctgccgcctt tggtcagctt
     7381 tttgccgact acgaactgct accaccgttc cgccagctcg accgtaacag ctacgccctg
     7441 acagaagccg agcgcaatgc cagtgaactg acccgctggg caggcagaaa atgcccgagc
     7501 ggtcgggtca tgggactggc gaataaaggt tgggtgcgtg gtgaaccgca ggatggtggc
     7561 tggatcggct ggatgatcaa acctctgggc cgctggtcgt taatcatgga aatcgatgaa
     7621 ggctttgcag ttggtatgtc gccagccgaa cttagcgccg agcaactctt aagcaagctg
     7681 tggctatggg aaggcaaagc agaaagctat ggctggggga gtaattcaac acaggaagcg
     7741 cagttctccg tactcgatgc catcaccgcc agcgagctga ttaacgatat tgaagccctg
     7801 tttgaataag gaaagagcat ggacaaggaa ttaccgtggc tggcggataa cgcccaactg
     7861 gaactgaaat ataaaaaagg caaaacacca ctcagccatc gcaattggcc gggcgaacca
     7921 gtgcctgtta tcactgaaag catcattcag acactgggtg atgaattgct acaaaaagct
     7981 gagaagaaaa aaaacattgt ctggcgttat gagaattttt cactggagtg gcagtccgcc
     8041 attacgcagg ccatcaactt gatcggcgaa cacaaaccct caatcccggc ccagacaatg
     8101 gcggcgctag tctgtatcgc gcaaaatgac agccaacagt tgctcgacga aatcgtccaa
     8161 caagaggggc tggaatatgc gactgaggtg gtgattgcac gccagtttat tacacgatgt
     8221 tatgagagtg atcctctggt agtcacattg cagtatcaga acgaggacta tggctatggt
     8281 tatcgctcag aaacctataa cgaattcgat ctccgactgc gtaagcatct ctctctggca
     8341 gaggaaagct gctggcagcg ttgcgccgac aaactcattg ccacactgcc aggaataacc
     8401 aaagttcgcc gcccttttat tgcgctgatc ctccccgaaa aaccagaaat cgccaatgag
     8461 ttggtaagcc ttgaatgccc gcggactcat tttcattcta aggagtggtt aaaagttgtt
     8521 gctaatgacc ccacagcggt gagaaaactc gaacgctact ggagccagga tatatttagc
     8581 gatcgagaag ccagctacat gtcgcatgaa aaccacttcg gctacgcggc ctgcgccgcc
     8641 cttttgcgcg aacaaggact ggcagccgtt ccgcgcctcg cgatatatgc ccataaagaa
     8701 gattgcggca gtctgctggt acaaattaac catccgcaag tcatccgcac cttgctgtta
     8761 gttgctgata aaaacaaacc cagcctgcaa cgtgtggcta aatacagtaa aaacttcccc
     8821 catgcgacac tcgccgcact ggcagaactg ctggcgttaa aagaaccacc agcccgccca
     8881 ggttatccaa tcatcgaaga caaaaagctg cctgcacagc aaaaagcacg cgatgaatac
     8941 tggcgtacgc tgttacaaac gctgatagca tcgcagccac aactagcaga aagggtgatg
     9001 ccgtggttaa gtactcaagc cagggcagtg ctgaatagtt atttatcggc accgccaaaa
     9061 ccggttattg atagcaccga taacagcagt ctgcctgaaa tgctcgtttc accaccgtgg
     9121 cgtagtaaga aaaaaatgac agctccacgt cttgatttgg caccgctcga attaactccg
     9181 caaatttact ggcaaccagg cgaacaagag aggcttgccg ccactgagtc tgcccgttat
     9241 ttcagcacgg aatctcttgc ggaacgcatg gaacaaaaaa gtggacgagt tgtattacag
     9301 gaactgggtt ttggggatga tgtatggctg tttctgaatt atatactccc cggaaaactg
     9361 gatgctgcac gcaattcact cattgttcag tggcattact accaggggcg ggttgaagag
     9421 atcctgaatg gctggaactc cccgcaagca caattagcag aacaggcgct ccgcagcggt
     9481 cacatagaag cgttaattaa catatgggaa aatgacaact tctcacgtta tcgtccggaa
     9541 aagagtgtct ggaacctgta tttattggca cagttgccgc gtgagatggc tttgaccttc
     9601 tggctgcgta tcattgagaa aaagcatctg ttcgcgggtg aggactattt tctcagtatc
     9661 ctcggattgg atgcgctacc aggtctgctg ttggcttttt cacatcgtcc aaaagaaaca
     9721 tttccgttaa ttttaaattt tggcgcaaca gaactggcgc tgcctgttgc ccgcgtctgg
     9781 cgacgtttcg cggcgcagcg tgatctggct cgccagtgga ttttacaatg gccggaacat
     9841 acggctagtg cacttatccc tcttgtcttt accaaaccca gcgataatag cgaagccgca
     9901 ttacttgccc tgcgtttact gtacgaacag ggacatggcg aattgctaca aaccgtggca
     9961 aaccgctggc agcgtacaga tgtatggtct gccctggagc agttgcttaa acagggtcca
    10021 atggacattt acccggcacg cattccaaaa gcccctgatt tctggcatcc ggcaatgtgg
    10081 tccaggcctc gacttatcac taataatcag cctgttaccg gtgacgctct ggaaattatc
    10141 ggcgaaatgc tgcgctttac ccagggggga cgtttttata gcgggctgga acaactgaaa
    10201 acgttctgcc agccacaaac gctggcagct tttgcctggg atctcttcac tgcgtggcaa
    10261 caagctggtg cccccgcaaa agacaactgg gcatttctgg cgttaagtct ctttggtgac
    10321 gaaagcacgg cacgggatct gacgacacag atcctcgcct ggccacaaga aggcaaatct
    10381 gcccgtgctg tcagcggcct gaacatcctt accctgatga ataatgatat ggcgctgata
    10441 cagctgcatc atatatcgca acgggctaaa tcccgcccct tacgtgataa cgcggcggaa
    10501 tttcttcagg tggtcgcaga aaatcgcggg ctaagccagg aagagctagc ggacagatta
    10561 gtcccaaccc tgggccttga tgatccgcag gcgttgagtt ttgattttgg tccccggcag
    10621 tttaccgttc gcttcgatga aaacctcaac ccggttatct ttgatcagca aaacgttcgc
    10681 cagaaaagcg ttccccggtt gcgcgccgat gacgatcaac tgaaagcgcc cgaggcactg
    10741 gcccgactaa aagggctaaa aaaagatgct actcaggtga gcaaaaacct gctcccgcgt
    10801 cttgaagctg ccctacgtac cacccgacgc tggtcgctgg cagattttca tactctgttt
    10861 gttaatcatc cctttacccg cctggttacc cagcgattaa tatggggcgt gtatccggca
    10921 aatgaaccgc gtcgtttact caacgccttt cgtgtggccg cagaggggga gttctgcaat
    10981 gcgcaggatg agccaattga cctgcctgcg gatgctctga ttggcattgc ccacccgtta
    11041 gaaatgacag cagaaatgcg cagtgaattt gcccagcttt ttgccgatta cgaaattata
    11101 ccgccacttc gccagttgac acggcgcacg gtgctgctca cgcctgacga gtcagccagt
    11161 aatagtttga atcgctggga aggtaaatct gctaccgttg ggcaacttat gggaatgcga
    11221 tacaaaggct gggagtcagg ctatgaggac gcatttgtct atgacctggg tgagtaccgg
    11281 ctggtcctta agttttcacc cggttttaac cactacaatg ttgatagcaa agcgctaatg
    11341 agcttccgtt ctcttcgagt gtacagtgac aataaatccg tcacttttgc cgaacttgat
    11401 gtgtttgatt tgagtgaggc gttaagcgca cctgacgtca ttttccatta aaacacagcg
    11461 ggcagtgatg caactgcccg ttatcaacac gacttaccag cggggaaagc gatgatcgtg
    11521 caaaaagagc tggttgctat ttacgattat gaggtccctg tacctgaaga tccgttttcc
    11581 ttcagacttg agatccataa atgctctgaa ttatttacag gttccgtcta tcgactggag
    11641 cgattccggc tacgtccaac atttcatcaa cgtgatcgag aagatgctga cccgctaata
    11701 aatgatgcgt tgatttatat aagagatgag tgtattgatg agcggaaatt acgaggtgaa
    11761 tcacctgaaa ctgtaatagc aatttttaat cgtgaactac agaatatatt caaccaacta
    11821 atagaatatt agtttattaa agggggaagc ggttttcccc cttagttgcg tttcaatttg
    11881 cccattactt caacaaatca atcaaccgta acagagacac taatagctcc agtacggcaa
    11941 tgagcacggt taacttttgt gtcagcgtgt ccatccgctc tccttattaa ggagcgcaac
    12001 accagcacct tacccttaca ctgcctgtcg atactggttt acgtttgttg agtcgccatg
    12061 ttgcactccg gatcagagca ctacctgcgg caccacccgt ccggtagcca ggacttgaaa
    12121 tgggttcgat ccggcgcaac ttcccaacag tcaggattat aaatctcatt atcttaattg
    12181 caacactgtc cagaggtggt tataattcgt tcgttgagat gccatgttgc actatcagta
    12241 acttaacatc aaagtagtaa ataaaagaac ctgcctcacc agcaggtttt tttattttct
    12301 gcgatctact ttccagatat tttttgccaa tagcccacca taaatattca catcccggtc
    12361 aaatggtgcg ttgaatataa aatcgatgaa tttataaata aaaatgagtt atatgatgaa
    12421 ttaataaaaa cagtaatcat tttattaaag ggggaagcgg ttttccccct tagatgcgtt
    12481 tcaatttgcc cgttacttca acaaatcaat caaccgtaac agagccacta atagctccag
    12541 tacagcaatg agcacggtta acttttgtgt cagcgtgtcc atccgctctc cttattaagg
    12601 agcgcaacac cagcacctta cccttacact gcctgtcgat actggtttac gtatgttgga
    12661 ccacgatgtt gcactccgga tcagggcacc acctgcagca ccacccgtcc ggtagccagg
    12721 acttgaaata gattcgatcc ggcgcaacgt cccaacggtc tggattataa atctcattat
    12781 cttaattgca acggggtcca gccgtggtta taattcgttc gttggaacac gatgttgcac
    12841 aggctgtggt gttaggcctg aaagtagtaa ataaaagaac ctgcctcacc agcaggtttt
    12901 tttatttact gtgatctgct ttccagatat ttttcgctca aacaactaat gcgccaaaca
    12961 tttattgcgc gtaaaatatc gtttatttca ttaatacatt tcagggatga atatatgtca
    13021 ccgcagaata atcatctgca gcgtccgcct gctgctgtgt tatacgccga tgaactggca
    13081 aaattaaaac aaaatgataa cgcaccttgc ccgcccggtt ggcagttaag tttgcctgcg
    13141 gcccgtgctt ttatccttgg cgatagcgcg caaaatatta gccgtaaagt ggtgattagt
    13201 ccctccgctg tcgaacgcat gttagtcact cttgccaccg ggcgcggttt gatgttggtg
    13261 ggggaacctg gcaccgcaaa atctctcctg tctgaattac tggcaaccgc tattagcggc
    13321 gatgccgggt taaccatcca gggcggcgca tctactaccg aagatcaaat caagtacggc
    13381 tggaactacg ccctgcttat caaccatggt ccgtcaacgg aagcactggt ccctgccccg
    13441 ctttaccagg gaatgcgcga cggcaaaatc gtccgttttg aagagataac gcgtacaccg
    13501 ctggaagtac aagactgtct gttaggtatg ctttctgaca gggtgatgac ggtgccggaa
    13561 ctcaccggtg aagccagtca gctctatgcg cgggaaggct ttaatattat tgccactgcc
    13621 aatacccgcg atcgcggcgt caatgagatg agtgccgcgc taaagcgtcg cttcgatttt
    13681 gaaaccgtct ttccgattat ggatttcgcc caggagctgg aactggtcgc cagtgcttcg
    13741 gcgcgtttgc tggcccatag cggtattccg cataaagtgc ctgacgcagt actggagtta
    13801 ctggtacgca ccttccgcga tctgcgtgcc aacggcgaaa agaaaacgtc aatggataca
    13861 ctgacagcga ttatgtccac cgctgaagcc gtgaacgtgg cccacgccgt aggcgtccgc
    13921 gcctggtttt tagcgaatcg cgcgggggag cctgccgacc tggtggactg tattgccgga
    13981 accatcgtca aagataacga agaagatcgc gctcgtctgc gccgttactt cgaacagcgt
    14041 gtcgccacac ataaagaagc tcactggcag gcttattatc aagcccgcca ccgcctgccg
    14101 tgaggaaaga ggcatgagcg agccgttaat tgtcggcatc cggcatcata gtccggcctg
    14161 cgcccggctg gtgaaatcgt taatcgaaag ccagcggcca cgatacgtgt tgattgaagg
    14221 cccggctgat tttaatgacc gggtagacga actttttttt cccaccagct tccggtagct
    14281 atttacagtt attgccagta tcaggacggt gcagcccccg ggcgtggtgc ctggacgcca
    14341 tttgctgaat tttcgccgga gtggcaggcg ctacaagccg cacgtcgtat tcaggcacaa
    14401 acttacttca tcgatttgcc ttgctgggcg cagagtgaag aagaggacga ttcgcctgat
    14461 acgcaagatg aaagccaggc cttactgctg cgtgccaccc gcatggataa cagcgatacc
    14521 ctgtgggatc acttgttcga agatgaaagc cagcaaactg cattaccctc tgcgctggcg
    14581 cactattttg cccaactgcg gggcgatttc cccggcgatg cactcaatcg tcagcgcgaa
    14641 gcctttatgg ctcgctggat tgcatgggcg gtgcagcaaa ataatggcga cgtgttagtc
    14701 gtctgcggtg gctggcacgc tccggcactg gcaaaaatgt ggcgcgaatg cccgcaggac
    14761 attaacacgc cggaattgcc ctcgctggca gatgccatta caggttgtta tctcacgccc
    14821 tacagtgaaa agcgccttga tgtgctggca ggataccttt ccggtatgcc tgccccggtc
    14881 tggcaaaact ggtgctggca gtggggctta cagcaggccg gtgaacaact gctaaaaact
    14941 atccttaccc gtttgcgcca gcacaagttg cctgcttcga ccgcggatat ggctgccgct
    15001 catctgcatg cgatggcgct ggcacagttg cgcggtcata cactaccgtt acgcactgac
    15061 tggctggatg ccatagcagg atcgctgatt aaagaagccc tgaatgcgcc gttgccgtgg
    15121 agctatcgcg gcgttattca tcccgacacc gatccgattc tgctaacgtt gatagacaca
    15181 ttagcgggtg acggattcgg taaacttgcc ccttctacgc cacaacctcc tctgccaaaa
    15241 gatgtcacct gcgaactgga acgtaccgca atctcccttc cggcggagct taccttaaat
    15301 cgctttaacc ccaatggact agcgcaaagt caggtgttac atcggctggc aatactggag
    15361 atcccaggga ttgtgcgcca gcagggaagc acactgacac ttgcaggcaa cggtgaagaa
    15421 cactggaaat taacccgccc gcttagccag catgcggcat tgattgaggc cgcctgcttt
    15481 ggtgccacac tccaggaagc cgcacgccat aaattagaag ccgatatgct ggacgcgggt
    15541 ggaatcggca gtatcaccac atgtcttagc caggcggcgt tagcgggtct ggcgtccttc
    15601 agtcaacaat tactggagca actcacatta ttaatcgccc aggaaaatca atttgccgaa
    15661 atgggccagg cgctggaagt gctatatgcc ttatggcggc tggatgaaat tagcggtatg
    15721 caaggcgcgc agatattaca aacgacgtta tgcgcggcta tcgatcgcac gctgtggctg
    15781 tgtgaatcta acggcagacc ggatgaaaag gagtttcacg ctcacctgca tagctggcaa
    15841 gcgctttgcc atattctgcg cgatctacat agcggcgtta atttatccgg cgtttcgctt
    15901 tctgcggcgg tagccttact ggagcgacgc agtcaggcga ttcatgcccc ggcgctggat
    15961 cgcggcgcgg ttcttggcgc actaatgcgt ctggaacatc ccaacgccag tgccgaagcg
    16021 gcgctgacga tgctggcgca gttatccccg gcacaatccg gcgaggcgct gcaaggtttg
    16081 ctggcattag cccgtcatca actggcctgt cagccgacat ttatcgccgg tttcagcagt
    16141 catttaaatc aactaagtga tgccgatttt atcaatgccc tgcccgattt acgcgcggcg
    16201 atggcctggc taccgccacg agaacgcgga acgctggcgc atcaggtgct tgagcattat
    16261 caactggcgc aacttcccgt ttcggcactg caaatgccgt tgcattgtcc accgcaagcc
    16321 attgcacatc atcaacaact cgaacagcag gcactggcat cgctgcaaca ctggggagtt
    16381 ttccatgtct gaactgaacg atcttctgac cacccgtgag ctacaacgct ggcgattaat
    16441 tcttggcgaa gcggcagaaa cgacgctttg tgggctggat gacaacgccc ggcagataga
    16501 tcacgcgctg gagtggctgt atgggcgcga tcctgaacgg ctccagcgtg gtgaacgctc
    16561 tggtggatta ggtggctcaa atctcaccac tcctgagtgg atcaacagta tccacacgct
    16621 gtttccgcaa caggtgattg agcggctgga aagcgatgcc gtactgcgct acggcattga
    16681 agatgtggtg acaaatctcg acgtgctgga acgtatgcag ccttccgaaa gcctgctacg
    16741 cgccgttttg cacaccaaac atctgatgaa tcccgaagta ctggctgccg cccgccagat
    16801 agtgcgccag gttgttgaag aaattatggc gcgactggca aaggaagttc gccaggcttt
    16861 ttctggtgtc cgcgatcgcc gtcgccgttc atttattcca ctggcgcgaa actttgattt
    16921 caaaagtact ctgcgcgcca acctgcaaca ctggcacccg caacacggca agttgtatat
    16981 cgaatccccc cgctttaaca gccgcattaa acgccaaagc gaacaatggc aactggtctt
    17041 actggttgat caaagcggat cgatggtcga ttcggtgatc cactctgcgg tgatggcggc
    17101 ctgtttgtgg cagttacccg gcattcgtac ccatctggtg gcgtttgaca caagcgtcgt
    17161 tgatctcacg gcagacgttg ccgatccggt agagttatta atgaaagtac agttgggcgg
    17221 cgggaccaat atcgccagtg ccgtggagta tggtcggcaa cttattgaac aaccagcgaa
    17281 aagcgtcatt atcctcgtga gcgattttta cgaagggggt tcatcatcat tactgacgca
    17341 tcaggtgaaa aagtgtgtcc agagcggcat caaagtgctg ggactggcag cgctcgatag
    17401 cacagcaaca ccttgctatg accacgatac ggcccaggcg ctggtaaatg tcggcgcaca
    17461 aatagccgcc atgacaccgg gcgagctggc atcatggctt gcggagaatc ttcagtcatg
    17521 aattcactac gtccggaatt attagaactg acaccgcagg ccctgacggc gttaagcaat
    17581 gccggttttg ttaagcgcag tcttaaggaa ctggaaaatg gcaacgtccc ggaaatcagc
    17641 catgagaaca gcgctttaat cgccaccttc agtgacggtg tccgtaccca gctggcgaac
    17701 agccaggcac tgaaagaggc tcagtgcagc tgcggggcca gcggtatgtg ccgtcatcgc
    17761 gtgatgcttg tgttaagtta tcaacgactt tgtactaccg cccagcctac ggaaaaagaa
    17821 gaagcgtggg atccggcaat ctggctggaa gaactggcta ccctgcccga tgccacccgc
    17881 aaacgcgcac aggcgttggt cgctaaaggc atcaccattg agttgttctg tacgccgggc
    17941 gaaattccct ctgcccgctt accgatgagc gatgtgcgtt tttattcccg cagcagtatt
    18001 cgtttcgccc gttgtgattg tattgaaggc acactttgcg aacatgtcgt actggctgtg
    18061 caggccttcg tcgaggccaa aactcagcaa gcggaattta ctcatttaat ctggcagatg
    18121 cgcagcgaac acgtcacatc atctgacgat ccgtttgcca acgacgaggg taacgcgtgt
    18181 cgtcaatatg ttcagcaatt aagccaggca ttatggctgg gcggcatcag ccagccgctt
    18241 attcactacg aggccgcttt cagtcgcgcg cagcaggcgg cggaacgctg caactggcga
    18301 tgggtgagtg aatcactacg acagctacgg gcaagcgttg atgccttcca cgcccgcgcc
    18361 agccactatc atgccggaga atgcttacgt cagcttgcgg cattaaacag tcgattaaat
    18421 tgcgcacaag agatggcccg gcgcgacagt gttggtgaag ttcctcctgt gccgtggcgc
    18481 acggtcgttg gctctggcat tgccggagaa gcaaagcttg atcatctgcg gctggtgtct
    18541 ttaggtatgc gttgctggca ggatattgag cattatggtt tacgcatctg gtttaccgat
    18601 cccgacaccg gcagtatttt gcacctttcg cgcagttggc cgcgaagtga acaggaaaac
    18661 tcaccggcag ctacgcgtcg gctgtttagt tttcaggctg gcgcactggc gggtgggcaa
    18721 attgtttcac aagcagcaaa acgcagtgcc gatggcgagc tgctgttagc tacccgcaac
    18781 cgcttaagca gcgttgtgcc gctgtcgcct gatgcctggc aaatgttgag cgcgccgtta
    18841 cgccagccgg gcattgtggc tttgcgggaa tatttacgcc agcgtccccc cgcctgcata
    18901 cggcctctta atcaggtcga taacttattt attctgccgg tcgctgagtg tatttcgctc
    18961 ggttgggata gcagccgcca gacgctggat gcgcaggtaa tcagcggcga aggtgaagat
    19021 aatctcctga cgttatccct tccggcatct gcctgttctc cttttgccgt tgaacgcatg
    19081 gcggcgcttt tgcaacaaac agacgacccc gtgagtctgg tttctggctt tgtcagtttt
    19141 gttgatgggc aattgacact ggaaccacgg gtgatgatga caaaaacccg cgcctgggcg
    19201 ctggacgcag aaactgcgcc tgtggcaccg ctaccttctg ccagcgtttt gcctgtgccg
    19261 tctaccgctc atcagttgct gatgcgctgc caggcgttac ttattcaact gctccataac
    19321 ggctggcgct atcaggaaca gagcgctatt agtcaggcag agttgctggc gaatgacctc
    19381 tccgcggtcg gtttttatcg gctggcacat gtgttgggac aatttcgtaa tacagaaagc
    19441 gaggcacggg tagaagcaat gaataacggt gttttacttt gcgaacaatt attccccatg
    19501 cttcagcaac aaggatgaaa tagtgctttt tactaagagt tctactccag ttccggactg
    19561 ctcacgtcac ggtattaggc atattctata tagcccctgg tgagagtcac cagtttcttg
    19621 attaaataaa atggagtttt acatgaaggc tttcaataag ctgttttccc tcgttgttgc
    19681 atctgttctg gttttctctc ttgctggctg cggtgacaaa gaagaatcga agaaattcag
    19741 cgccaatctg aacggcactg aaattgccat tacctatgtc tacaaaggtg acaaggtgct
    19801 taagcaatct tctgaaacca aaattcaatt tgcttctatt ggtgcaacca ccaaagaaga
    19861 cgctgccaag acacttgagc cgttaagcgc caaatacaaa aacatcgcgg gtgttgaaga
    19921 aaaattaacc tatacagata cctacgcgca ggaaaacgtg actatcgata tggaaaaagt
    19981 ggattttaaa gccctgcagg gtatttcagg aatcaacgtt tctgctgaag atgccaaaaa
    20041 aggtatcact atggcgcaaa tggaactggt gatgaaagcc gctggtttta aagaagtgaa
    20101 ataatctgtc ggcggccatg tttcgcatgg ccgccatacc cgtcttagct tttcttcaca
    20161 tgctggcgcg ctgccagtcc acgcagaaaa taacgtaaaa attgatcgcc gcattcgcgg
    20221 aagtttttat gatccggtgc gcgcatcatc gctgtaattt ccggcatcga aacgcggaac
    20281 tgctgttcgg tgaggatagc cagaatgtca tcggttttca gcgaaaacgc gatgcgtaat
    20341 tttttcagca cgatgttgtt attaatgcga cgttccggct ccagtgccgg agcagactca
    20401 tccttgccgc gtttttcata aatcaggcca ttgaggaatg acgacaaaac aatgtccgga
    20461 caacgctgaa aaccctcttc gtcttcttta cgtagccaga cggcgatctg ttccgcggtg
    20521 gcttcgacat tacccagcgc cagaatacgc accaggtcat tattattggc tttcaaaatg
    20581 tagcgcacgc tgcgcagaat atcgttactt agcatgaggc cttcaggtgt tgatgaggca
    20641 aaaagccatt ttagcagtct tttacaggcc aatcgcctct tttaagcttt tcagataacg
    20701 gcggctgacc ggcacggtta agccattacg caaaatcaac tcggcctggc cgttatcttc
    20761 cagacgaatc tcctgtaaat gcgcgaggtt aaccagatac tgacgatggc agcgcagtag
    20821 tggtgtacga ctttccaggg tacgtaatgt caattcggta aagccctctt tcccttcgtg
    20881 gctggtaacg tagacaccgc tcatccgact gctgacaaat gccacatctt tcatttgcag
    20941 caaataaatc cgactatgcc cagtacaagg gataaatttc agcgcctgtt gattttctgg
    21001 taacagcgaa acatcctgct tgctgcgttc ctgccgcaaa cgcgccagcg ttttctccag
    21061 tcgcgcttca tcaattggct tcagcagata atcaaaggcg tgttcttcaa aggctttaat
    21121 ggcgtattcg tcaaacgcgg tgagaaaaac aatatacggg cgatgttccg ggtcaagcat
    21181 ccccaccatt tccagaccac tgatgcgcgg catctggata tcgagaaaca gcacatccgg
    21241 gcgcagttta tgcaccgcgc cgatcccttc cacggcgttt gaacactctc caacgatttc
    21301 aatatcgctc tgctcctgca aaaatacgcg caggttctcc cgtgctaacg gttcatcatc
    21361 gacaattaag actttaatca tgcctcgtcc ctccatggta gtcgtaacgt tattcgggtg
    21421 taactatcag gctcacaggc gacgcttatc ccatagtcat cgccaaaccg ttcacgtaaa
    21481 cgcttatcca ccagattcat ccccagccca ctggcattgg ttaccggttg atacaaaccg
    21541 gcattgtctt cgatctccag catcaaatgt tgcccctcac gtcgggcgct gattgccact
    21601 cgccctgtat ccagcagttg tgatgtccca tgtttaatgg cgttttccac tatcggttgc
    21661 agggtaaacg cgggcaattg ctgctgggat aattcttgcg gaatagcaat gttgacctgc
    21721 aaccgcgact ggaagcgcgc cttttcaatt tgcagataag cgttcacatg ttcaatttcg
    21781 tcggcgagag taacaaactc cgaaggccgc tttaagtttt tgcggaaaaa agtggaaaga
    21841 tactgcacca gctggctggc ctgttcgctg tcgcggcgga tcaccgcttt aatggtgtta
    21901 agcgcattaa acaaaaaatg aggattcacc tgggcgtgaa gcagtttgat ttctgactgg
    21961 gtgagcatcg ctttttgccg ctcatattgc ccggcaagaa tctgcgccga aagcagttgc
    22021 gcaatcccct cgcccagcgt gcggtttatt gaactgaata aacggttttt ggcttcatac
    22081 aatttgatgg tgcccatcac gcgctgattt tcgccacgca acggaattac cagcgtcgac
    22141 cccagtttgc attgcggatg caaagagcaa cgataaggta cttcgttgcc atcagcgtag
    22201 accacttcac cggtttcaat cgcttttaag gtgtaagtcg aagaaatcgg tttgccgggt
    22261 aaatggtggt cgtcaccaat tccggtaaag gccagcaatt tctctcgatc ggtaatcgcg
    22321 actgcaccaa tatccagctc ctgatacagc acctgagcca ctttcatgct gttcacttcg
    22381 ttaaacccct gtcgcaaaat gccttccgtc gaggctgcca ctttcagcgc agtggcagaa
    22441 aaagccgaag tgtatttttc aaacatcgcg cgtttatcga gcaatatacg cataaacagc
    22501 gccgcgccga cggtattggt gaccatcatt ggcgcagcaa tattactcac cagacgcacc
    22561 gcatcttcat aaggtcgggc gatcgcaagg atgatcagca tttgcaccat ttcagcgacg
    22621 aacgtgacgg caccggcggt aatggggtta aagactttat cagtgcgccc gcgacggatc
    22681 aggatgctgt gtaccaggcc gccgagcaat ccttcaacga tggttgagat catgcaactt
    22741 agcgcggtca tgccccccat cgaatatcga tgtaagccgc cggtcagacc aaccagccca
    22801 ccgacgaccg gaccgccgag taagccgccc attaccgcgc ctatcgcacg ggtattggca
    22861 atagaatcgt caatgtgcaa cccaaaccag gtgcccatga tgcagaagat ggaaaagacg
    22921 atgtagcaga gaaatttatg cggcagacga accgtgacct gcattaacgg tatgaataat
    22981 ggcgttttac tcattaacca cgcaatgact aaaaaaacgc acatctgctg aagcagcagc
    23041 aacaccagat taaaatcgta catacccgca aaccacactt ccctttaaaa cgcgtaacat
    23101 acattgcctg cgtttaactt tctttgaact cttgcagaaa aatgagaatt cgtgagtacg
    23161 atcactcaaa atcgcctggc aaaaataaaa tcaccctata gatgcacaaa aaacgggcaa
    23221 aactacctgg ttcgcaaaac tgcgtctaaa gttaaactgg gacctcgcga gcaagggtga
    23281 gacgatggcg ctttacacaa ttggtgaagt ggcgttgctt tgtgatatta accctgtcac
    23341 gttacgcgcg tggcagaggc gttacgggtt gctgaaaccg caacggacag acggcggtca
    23401 tcgactgttc aacgatgccg atattgaccg gatccgcgag atcaaacgct ggatcgacaa
    23461 cggcgtgcag gtcagcaaag ttaaaatgct gctcagtaat gaaaatgttg atgtgcagaa
    23521 cggctggcgc gatcagcaag aaacattact gacctacctg caaagcggca atctgcatag
    23581 cctgcgaacg tggatcaaag agcgcggtca ggattacccc gcccagacac tcaccacgca
    23641 cctgtttatt cctctgcgcc gacgacttca atgccaacaa ccgactctcc aggcgctgct
    23701 ggcgatcctc gacggcgtac tgatcaacta catcgccatt tgtctggctt cggcacgtaa
    23761 aaaacagggt aaagatgcgc tggtggttgg ctggaatatt caggatacca cccgtctgtg
    23821 gctggagggc tggattgcca gtcaacaagg atggcgcatt gatgtcctcg cccactcgct
    23881 caatcaacta cgccctgaac tattcgaagg ccgtacattg ctggtgtggt gcggtgaaaa
    23941 tcgaacctcc gcccaacagc aacaactcac cagttggcag gaacaaggcc atgatatttt
    24001 cccactcggc atttaatgat tcgttaacaa atgcgcttta ctgtacaatc ctttcgttaa
    24061 cataaggagt gcattatgcg catagctaaa attggggtca ttgccctgtt cctgtttatg
    24121 gcgttaggcg gaattggtgg cgtcatgctc gcaggttata cctttatttt gcgtgctggc
    24181 taagcgcctg caccagcctt tcaaacaggc ggtctgcgat gatcgccgcc agtgccacca
    24241 gtaacgcccc ctggatcaca tacgcggtat taaatccgct aagcccgatg atgatgggcg
    24301 tacccagcgt gctggcccct accgttgagg cgatcgtcgc cgtaccaatg ttgataatca
    24361 ccgaagttcg cacgcccgcc agaatcaccg gagccgccag cggtagctcg accttacgca
    24421 gtcgctgacc acgactcatt cccatacctt tcgcaacttc tgtcacgctg gcatcaatcg
    24481 ctcccagccc ggcaagtgtc gcctgcagga cgggcagcac accgtaaagg atcaaggcga
    24541 taatcgctgg ttgcagacca aagccgatca ccggaacggc gatcgccagc actgcgacgg
    24601 gcggaaaagt ctgtccaacg gcggcaatag tttccaccag tgggcgaaat tccgcgcccc
    24661 acgggcgagt gacagcaatt ccggcaccag taccaatgat caccgcaaac aaactcgaaa
    24721 ttcccaccag ccagaaatga gccagtgcca gagctgcaaa actttcttgc tgataaacgg
    24781 gtcgtggcag ttgtgggaac aaggcagcaa acagcggctg gctgtaaggc agccagaaaa
    24841 tcagtgccac aaacaaagca atgagccaga acagcggatc gcgcaacatt ttcatacgct
    24901 tacgcctcct ccagcagatc ctgaaaatgc agcgtgccgc aaggctggcc ctgcgtgttc
    24961 atcaccggca gcacctcgca tccccgcgcg acaaacaggg agagcgcatc gcgtagcgtc
    25021 atctcttctg ccagtgcctc accttctgcc cgttcttcgc gacgcacgta atccgccaca
    25081 ctacgtaacg aaagcaggcg cacccccagt tcactacgtc caaaaaactg gcggacaaaa
    25141 tcgtttgccg gacgagtcag catcgtcagc ggattgccct gctgcaccac ttcaccgtga
    25201 tccatcaata ccagatgttc tgccagccgt agcgcctcat caatatcatg agtgaccagc
    25261 acaatggtac gccccagcaa acggtgaatg cgcgtcatct cttgttgcaa cgcgccgcgc
    25321 gttaccgggt ccagtgcgcc aaaaggttca tccatcagta agacttgcgg atcggcagcc
    25381 agtgcgcgcg ccactcccac acgttgctgc tgaccaccgg aaagctgatg cggataacgc
    25441 tcacgtaaat ttgactccag ccccagtagc gccattaatt cgtcgatacg atcgtcaatc
    25501 cgcgcccgcg accatttttg taattgcggc acggtagcga tgttttgcgc cacgctccag
    25561 tgggggaaca ggccaataga ttgaatggca tagcccatcc ggcggcgcaa ctccagtact
    25621 ggcagcgagc gaatttcttc tccggcaaag cggatcactc cgctgtcatg ctccaccagg
    25681 cggttaatca ttttcagggt ggtggatttg ccggagccag atgtgccaat cagcaccgaa
    25741 aaactccctt cctgaaaatt gagattgaga tcgttaacgg ctttttgtgc gccgaacagt
    25801 ttgctgacat ggctaaattc aatcattacg tttcaccttc agcagtgcga taagcaaatc
    25861 gaacagcgcg tcggtaagca ccgccagaac aattaccggg atcaccccca gcaacactaa
    25921 atcaatggcg ctgcttagca gcccctggaa aaccagcgca ccaaaaccgc ctgcgccgat
    25981 taacgccgca atcaccgcca tacctacagt ttgcaccatc accacccgca ggctgcgcag
    26041 aaataccggt aacgccagtg gtaactgaac atgcaggaat cgctgcgccc cgctcatgcc
    26101 catcgctctg gcgctctcca gcacatcgcg cgggatctgg ttcaaaccga ctaccacgcc
    26161 gcgcaccagc ggcagcaagg catagagcac cagcgcaatc agtgcgggtg tcattccggt
    26221 tcctgctatg ccgagcttcc ccagccacgg aaaggccgtc accagcgcgg caagcggcgc
    26281 aatcaacagg ccaaagagcg ccaccgaagg cacggtctga atgacattga gcagagaaaa
    26341 aattgccccc tgccgagcag tggaaaagta gcaccagatg cccaacggca caccaatcac
    26401 taacgcaggc agcaccgcac caaacagcaa cgtcagatgt tgtgccagcg cgtcgtcaaa
    26461 cacatcctga cggttggcgt attctttcat tagtgagaga tcgttaagcg tgccggagta
    26521 cagcaaccac agcggaataa tggcaatctg catatgcaac aaccagcgcc acagcggatg
    26581 cgtggagatt cggcggatgg catcgctaca ggccagcaat gccagcgccg cagccagcca
    26641 gaaaccactg ccgaggctgg tacgcgccag cgcactgcca ttttgcgcca gttgtgtcgc
    26701 cgcctttccc gcgccccaca ccagcaatac gaagacgaat tgcgccagaa tgagtgcaca
    26761 aatgctgcct tttttaccgg gaataaaaca ggccgtcagc caggcgcaac caacgcccac
    26821 cagcatccag atcgtttgcg gccacagctg ccagagatga cgcccttcac ccgagactaa
    26881 acgattaggc gcgtaactga taaacggcag cgccgctgcg attaccgtca gcaacagcag
    26941 cagcgccaga acaggattaa tacgtagata agtcacggga aattacttcg tccacccttt
    27001 ttgtttcagg tagtcggcag ccactttttt ggcatccagt ccttccactg caatgctggc
    27061 attcagttgc tgcaatgttt ttgcatcgag gctggcgaag actggctgta gccactgtgc
    27121 catttgcgga tactctttca gcaccgactc acgcaccact ggtgcaggcg cgtagatagg
    27181 ttgcacgcct tgcggatcgc ttaaggtttg cagccccagc gccgcgaccg gaccgtcagt
    27241 gccgtaagcc attgcggcat taacgccaga agtttgctgg gcagcggctt taatcgttac
    27301 cgccgtgtcg ccgccagcca gtgacagtaa ctgatcctga tcgagtttaa agtcgtaggc
    27361 tttttcaaac gcgggtaacg catcggcgcg ttcgataaac tctgccgagg ctgccagttt
    27421 gaaggtgccg ccctcttgca gataacgact caggtcggca agcgaagtga gtttattttt
    27481 ctctgccaca tcctgacgca cggcgatagt ccaggtgtta tttgcaggcg cgggcgtcag
    27541 ccagattaac ttgttgtgct ccgaatcgag ctttttgact ttctcgtaac cttgctgcgc
    27601 gtttttccac gctgcatcgt tttcatcttt aaagaagaaa gcgccattgc cggtatattc
    27661 cggatagata tccagttcac cggaagtaat cgccccgcgc accacaggag tcgttccgag
    27721 ttgcacttta ttcaccgtcg gtacgccgtg gctttccagc acctgcaaaa tgatattgcc
    27781 gagtagcgca ccttcggtat cgatttttga accgacttta acgggggaag ccgcctgtag
    27841 cggcaggctc acggctgcca acataaccag tgaacctgcc cagagcttta agagtggcat
    27901 gatgctttcc tcattctttt actgttgttt tcagcgaatt aagagaaaag catagttgat
    27961 aatggcgggg ttagcttgag cggaaggatt cagttgcaga atcagataaa tacttaagag
    28021 gcatattcgg tccggcgttt cctgccggat gcggcgcgag cgccttatcc ggcctacaaa
    28081 gggcgcaaac gtcgcgccct tactaaagca ttacagcaac tcaaactcgc ctttcttaac
    28141 gcgtgcggaa tcagtgccga taaatacatt gaacttgcca ggctcggcgt catatttcat
    28201 ctgttgattc cagaacttca gcgcctcaat atcgatcggg aagctgacag tctgagtttc
    28261 gcccggtttc agggtgattt tctcaaagcc tttcagctgt ttcacagggc gactcatgga
    28321 agccgtcaca tcctgcaagt acatctgcac taccgtggcc ccctcgcgct taccggtgtt
    28381 cgtcacctgc acgctggcag tcactttgcc gtcacgcttc atggtcggcg cagaaagttt
    28441 cacatcagag acggtgaaag tggtgtagct cagcccatag ccgaacggat acaacgcccc
    28501 gttagcttca tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat tatacgggcg
    28561 accggtattc agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggacat
    28621 tggcagcttg ccggacgggt tgtaatcgcc aaacaataca tcggcaattg cattaccgcc
    28681 ttcagtcccc gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag
    28741 cgccagcgga cgcccgttca tcagcaccag caccagcggt ttaccggtgg ctttcagcgc
    28801 cgcaatcaag tcacgttggc tttgcggaat agtgatatcg gtccggctgg aggcttcgtg
    28861 cgccatcccc tgtgcttcac cgactacagc caccaccaca tcagattgtt tcgccgtctg
    28921 caccgcttca tcaatcatct cttgcggcga acgcggatcg actttgaccg cttcttcata
    28981 ctgattcagg aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag
    29041 cactttaccg ttttcaccga cggaattttt aatcccggtc agtacggtca cggattgatc
    29101 ggcaacaccg gctgcggacc agctgcccat cacgtcacgt ttactgtccg ccagtggccc
    29161 aaccaccgca atggtggccg attttttcag cggtaacgtt tcgagacggt ttttcagcaa
    29221 caccaggctt tcgcgcgcca cttcacgcgc ttctttacgg tgcaggcggc tttcggcatt
    29281 ggtatccacc gggtcagact ctttcggccc caaatggctg tatgggtcgt taaacaatcc
    29341 catatcatat ttaacgttca gtacatggcg ggcagcatcg tccagctctt ccatcgtcac
    29401 tttgccggat ttgatcaacc caggcagata cttcgagtaa tactcgtcgc tcatactcat
    29461 gttgattccg gatttcagcg ccacgcgcac cgcatcttcc gggtctgccg ccgtgccatg
    29521 tttaatcagc tctttgattg caccgtgatc ggaaacggtg atgcctttaa agccccactg
    29581 gtcgcgcaga acatctttca gcagccagga atcggaggtg gctggcgtgc cgttcagcga
    29641 gttcagcgcc accatcaccg cgccgctgcc tgcgtccagc cccgctttgt acggcggcat
    29701 ataatcatta aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc
    29761 taccgcgccg tatgcggcaa agtgtttgac gctggtcatc accgagtagc gatctgccgg
    29821 gcttttaccc tgcatcgctt ccaccatggt tttacccatt gttgaggtga gatacgtatc
    29881 ttcgccaaaa ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg
    29941 tgcccaggtc atattcaggc catcatctgc cgcttcataa gcagagacac gtccgaccgt
    30001 tttcactgca tcgaggttaa aagacgaggc cagaccgagg ctaatcggga acaccgtgcg
    30061 ctgaccgtgc agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat
    30121 cacctgatcc tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tcgccccaac
    30181 ctgaccgtct ttgatcatct cgcggatcgc ctctttcggg ttatccgggc cgacgctgat
    30241 taagcgcagc tgaccaattt tctcatcaac tgtcattttc ttaagcagtt cggtgacgaa
    30301 cgcatcccgc gcttcgggcg ttaatggatg gttgccgaat aaatcatccg ccagtgctgg
    30361 ctgcagggcc agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct
    30421 tcctctgttg cccgacgagg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat
    30481 gatggagaga gaaaaagaat gctcagttta ttgtctgaat tttcaaaata ttcactcgct
    30541 gaattgtcat acaaggcgct attctagttt gtgatatttt ttcgccacca caaggagtgg
    30601 aaaatgtctt ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg
    30661 gtcggtcatt cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc
    30721 cgttctggtc agggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg
    30781 cgggtgctga aagcctgtgt caccgccgac aaaattattc tgatgcaggc tgccaataca
    30841 ggcctgaccg aaggatcgac gccaaacggt aacgactatg atcgcgatat cgtgatcatc
    30901 agcaccctgc gtctcgacaa gctgcacgtt cttggcaagg gcgaacaggt gctggcctat
    30961 ccgggcacca cgctctattc gctggaaaaa gccctcaaac cgctgggacg cgaaccgcac
    31021 tcggtgattg gatcgtcgtg tataggcgca tcggttatcg gcggtatttg taacaactcc
    31081 ggcggctcgc tggtgcaacg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata
    31141 aatgaagacg gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg
    31201 gagcagatcc ttagcaagct ggatgatgat cgcatcaaag atgacgatgt gcgtcacgat
    31261 ggtcgtcacg cccacgatta tgactatgtc caccgcgttc gtgatattga agccgacacg
    31321 cccgcacgtt ataacgccga tcctgatcgg ttatttgaat cttctggttg cgccgggaag
    31381 ctggcggtct ttgcagtacg tctagatacc ttcgaagcgg aaaaaaatca gcaggtgttt
    31441 tatatcggca ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaac
    31501 ttcgaaaatc tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa
    31561 aaatacggca aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc
    31621 ttctttaatc tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg
    31681 cattttactg accgtgcgat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg
    31741 cgcatgaaaa actggcgcga taaatacgag catcatctgc tgttaaaaat ggcaggcgat
    31801 ggcgtgggcg aagccaaatc gtggttggtg gattatttca aacaggccga aggcgatttc
    31861 tttgtctgta cgccggagga aggcagcaaa gcgtttttac accgtttcgc cgctgcgggc
    31921 gcagcaattc gttatcaggc ggtgcattcc gatgaagtcg aagacattct ggcgttggat
    31981 atcgctctgc ggcgtaacga caccgagtgg tatgagcatt taccgccgga gatcgacagc
    32041 cagctggtgc acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac
    32101 atagtgaaaa aaggcgtgga tgtgcatgcg ttaaaagaac agatgctgga actgctacag
    32161 cagcgcggcg cgcaataccc tgccgagcat aacgtcggtc atttgtataa agcaccggag
    32221 acgttgcaga agttctatcg cgagaacgat ccgaccaaca gcatgaatcc ggggatcggt
    32281 aaaaccagta aacggaaaaa ctggcaggaa gtggagtaaa aattacggat ggcagagtat
    32341 cgccatccga attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg
    32401 ctttttgttt tttatagctc aacgctgctg ccggcacagg catcacttta ccggtttcaa
    32461 tccaggtacg caggcggctg gcatcggcaa aatgggtata tttgccaaac gcgtccatca
    32521 ctaccagcgc caccggttta ttattgataa ccgtacgcat caccagacaa tggcccgccg
    32581 cattggtaaa gccggttttg gttaactgaa tattccagtt atcgcgatac accagatgat
    32641 tagtattgcg gaacggcagc gtatacgtcg ggttagagaa ggttgccata tcttcccggg
    32701 tagtacttaa ctgcccgatc aacggatatt gtttgctggc aatgagcagt ttggttaagt
    32761 cacgggcagt tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt
    32821 tcattccgag cgatttcgct ttcgcattca ttgccttaat aaaggctttg taaccaccgg
    32881 gataatggtg tgcaaggctt gccgccgcgc ggttttctga agacatcagc gccagcaaca
    32941 gcatatcttt acggctgatt tcgctattca gtcgtacgcg cgaatagacc cctttcatct
    33001 ccggcgtctg gctgatatcc acttttagtt tttcatccag cggcagtcgt gcatccagca
    33061 caaccatcgc ggtcattaat ttgctgatag acgcaatcgg acgcaccaga tccgggtggt
    33121 tcgaatagat cactttgttg gtattcagat caacaatcat cgcgctaccg gaggcaattt
    33181 ccggttgtga agcggtggta gcggctgccg ttttcgcaac ggcctgcggt gcaaaaggca
    33241 cagccagcat cagggccagg ctaaataaag aaactcgaaa tttcggcatg atgagcattc
    33301 agatagtggt tcacgcgcac gggttgcgca ccgccggagt aaggatttac tgaggctagc
    33361 gacgccatca taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag gaaatgctgc
    33421 attgctgaca tttacgccag caatgcaacg tcagagaact ttctagaaca aacgataacc
    33481 gtagccccat agtataacgg ttagggcgag cagcacttcc agtaccagca cgccaatcgc
    33541 cagcgtcgaa ctagagaagc taaggccttc ctctttgttg atattcagga agctcggaat
    33601 accaaggtaa agcaggtagc cggtgtaaaa cagcgccacc gtgccgacca gcgcacacaa
    33661 ccagaccagt ggataaagcg ccaccagacc gcttaaaaac agcggagttg caacgtagcc
    33721 cgcgaagacc atgcagtgcg caagtgacgg acgctgcgga taattacgcg ccatccacca
    33781 gatgacccgc cccatcaccg cgaccccagc cagcataacg ccgtaaaaca agacagccag
    33841 cgccagtccg gtaaaccagg ataacttcag gatagtgcca tcgccaaaat tccagccaat
    33901 ctgtgtagtg ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg
    33961 ggtgtaatgg tgagaaatcg tttcgttttc gcgattaatc acctgcattt cacgatcggg
    34021 atgggaaaac agtccccaga catggctcat accgccccct tgttgtgagt tcatgaacct
    34081 gacagttcaa gtataagtca gcttgtgatt attttttgtt cgcccatgta attttcactg
    34141 tctgatgaac gtcctttttc agcgtgaatg attcacaggg tgtatgctta ctggcaacca
    34201 aagggagaca gactggccta tggatctcaa tacacttatc tcacaatatg gttatgccgc
    34261 gctggtgatc ggtagcctgg cggaaggtga aaccgtgact ttgctgggag gcgttgcggc
    34321 gcatcagggg ctattaaagt tcccgctggt ggtactttct gtggcgcttg gcggcatgat
    34381 tggcgaccag gtgctctatc tgtgcgggcg gcggtttggc ggcaagctgt tacgccgttt
    34441 ctcgaaacat caggataaaa ttgagcgggc gcagaaactt atccaacgcc atccgtatct
    34501 gtttgtcatt ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg
    34561 tgccagccag ctgccgccga aaatctttct gccgctgaat attctcggcg catttgcctg
    34621 ggcgttgatt tttaccacta ttggttacgc tggtggtcag gtgattgcgc cgtggttgca
    34681 caatctcgac cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt
    34741 gggcgtgcgc tggtggctga aacggcgcgg gaagaaaaag ccggatcatc aggcgtaaaa
    34801 ccattgccct gggtaaggcg ttcgcgccgc atccgacatc cggtgcacca tgcctgatgc
    34861 gacgcttgtg cgtcttatca tgcctaccaa aattttaccc attgaaataa ccacgcacct
    34921 cactgcgcta ttctgggttg aactgcggat tcgccaacat aaagccgcca tccactatca
    34981 acgactgccc ggtggtgtaa tttgcgccct ctgagcacag ccacgctacc aggctggcaa
    35041 tctcatgcgt tgcgccaaaa cgccgcagag gaatcgaagg ctccgcgtcg ggcttcacgt
    35101 cgctgtcatc catgccattc attggcgtgg cgatcgcccc aggcgcgact gcgttcacca
    35161 aaatcttatg cctgaccagc tccagcgcca tcgttttggt taacccgccg agcgcatgtt
    35221 tagcggctgt gtaagcgctg gcatccggca gcggcgtatg ttcatgtacc gacgtaatgt
    35281 tgatgatgcg accgccctgc ccttgtttca ccatctggcg ggccgcaatt tgtgagcata
    35341 aaaatgcacc atcgacatca acggtaaaaa tcttgcgcca ctcatcgaaa gccatatcaa
    35401 gaaacggcgc tttggtcatt gcgcccgcat tattcaccag cacatcaata cgccccagcc
    35461 gttgaatgag tttctccagc gcctgcgcac cttctggcag tttaccgaga tccagctgca
    35521 cgatctccgc acgtacgccg tggctaacta cctcacgcgc ggtatctttt gccccttctt
    35581 catctgagtg ccaggtaatg ccaatatcaa acccctgctg cgccagtaat aacgcgcact
    35641 ctttgccgat ccccgaatcg gaggcggtaa taatcgcaac ctgtgccatc gagttctcca
    35701 cttaacgctg aataaacgtt aagtatagaa ggcgcatatc atcagcgttt gtaacccccg
    35761 cccaacgcac cagtgagttg aatggaagca tccagccact gcccttgcaa taacaggcca
    35821 ttggcccgct cacgcagcgc ggggattctg gcttcgctga cgcgggaacc agcaatgatg
    35881 cccgcgttaa agcgcgcctg cgcaagacct accacacgca aggcatcgcg ctcaatttgc
    35941 gcctgatgct ggtttttctc cgcaagtgtc tgaacctgac tggctgcccg cgccacgtca
    36001 ttcaccgctt caaccaccgc tttgttgtag ctggcgatag acaagttgct ttcggctttt
    36061 gcgatatcga gattggcgtt aagacgacca ctatcgaaaa tgggtagcgt caggcctgcc
    36121 gtaacgccca tttgctgcgc ggaatgacgg aacagatcgc ttaagtgcaa cgcatcctgt
    36181 tgcaggaagg ccatcaggtt gatgtcagga taaaatgccg cttttgccgc atcaatggtg
    36241 cttagcgatg actcaacgta ccagtgcgcc gcctgcaaat ctgcccgccg ggccagtaag
    36301 gagtacccca gttcatcagg aagctggctt gccactttcg gcaacgcgac cggtttaagc
    36361 ttcaatgact ttgtctggtt atttgtaagt gcgcttaacc gtgcctcaat aattttcatt
    36421 ttccccgcga catcgttgag ctgctgccgg gttttgctgg cattaatatc ggtttccaca
    36481 ccttcaactg aagaagtaat cccgttctga tatagctggc gatcggtcgc gataatggtg
    36541 ttctgctctt tttctatttg ctgcaagacc gtgtttaacg ccgcctgggt ttgccactcc
    36601 cagtacaggc gggctacgct gccagccagc aattggcggg tttgctcgcg ttccgccgcc
    36661 cgtgctttaa ccgtacccag gcgggcagta acctccgccc gattctttcc ccagatatcg
    36721 aaatgccagc ccgccgttaa gccaaaagtt ccgttggtgt accacggacc ggtcgtacct
    36781 gcggccggat cgttcagagc aaacggcccc attaagcctt ctgccgacat tttttgccgc
    36841 tccatatccg cagaaaagtc gatctgcgga ccatcctgag tagcaattgc cttcgcctgg
    36901 gcttcagcta gctgaatgcg ctgttcagcc acctgcatat ccggtgcgtt ctgtagtgca
    36961 ttgttaatta aggaagtgag ttgattatcg tgatactcca gccaccattg gctgtctggc
    37021 caaccatttt tcagcgccgt gggtaatgcg gtgtcaactt gtgcagcagg cgtttgctgg
    37081 cttaacgcct ggcgggtttc atgcataggt gcacacccgg ccagcatcag taacagcgga
    37141 aaacaggcga tggctggata aaaggaatca cgattcatgg gggaataatc aggtaagaaa
    37201 aggtgcgcgg agattaccgt gtgttgcgat atatttttta gtttcgcgtg gcaatacatc
    37261 agtagcaata aaacgacata tccagaaaaa tatagactaa gtaaatgata tcttccgatt
    37321 tatcttaatc gtttatggat aacggcaaag ggcttcgttt tttcctatac ttattcagca
    37381 ctcacaaata aaggaacgcc aatgaaaatt atactctggg ctgtattgat tattttcctg
    37441 attgggctac tggtggtgac tggcgtattt aagatgatat tttaaaaatc agatatggca
    37501 tataagttgt caggtcagga actagatatt ttgtgtactt gatttgcaaa cagctccggt
    37561 gttggtggcg tcaaaatcgc tgaagcgttc cccgaaggcc ggggcagccc acatggatgt
    37621 gggctgaggg cgcgtcttac aaggacgtta cctcgcgccc gacccggtag acgaagggga
    37681 taagctgaag gtaccgcgca gcggcgattt tgttcgccag agcccggggg tgcaggggga
    37741 ggaggcgatt gtcctccccc tgcgcgctcc ttgcgccagt ggcaatatgt ggcttagctc
    37801 atgaaaggag cgcaacaaga tgatgaagct gaataagcga ttcattgtct ctgataaact
    37861 gcgattccac tattatcgca gcttatcgat atcaattgcc tgaatagccc ttgcaatatc
    37921 aggggaatta ttcaacaccc gaacatgctg aaataattcc gttgcttcat cgtatttttt
    37981 acgcaaataa ctcaaccact gtttaatccg cgcaacgtga tataacccgg tatcgccctg
    38041 cttttccaga cgggtatatt tttgcagcaa agcaaccacc tccggccacg gcattcgcgg
    38101 ttcgttatat tttactaccc ggctcaggtt gggaatattg agcgccccgc gaccaatcat
    38161 cactgcgtcg cagccgctga tcgcaatgca ttgttgcgcg ctctgccagt cccagatttc
    38221 gccgttggca atcaccggaa tattcagccg ctggcgaatc tcgccaatcg cctgccagtc
    38281 aatatgctcc gcgcggtaac cctgctcttt tgtccgtcca tgcaccacca gctccgtagc
    38341 gccagcctgt tgaaccgcat cggcgatttc aaatttcttc tcaccgctgt cccagcccag
    38401 acgcactttt acgctgacgg gcaaatgcgc tggtacagct tcacgcatcg cttttgcacc
    38461 ctggtagatg agttcaggat ctttgagtaa cgtcgcgccg ccgccgctac cgttaaccgt
    38521 tttcgacggg cagccgcaat tgagatccac gccccaggaa cctaactcca ccgcacgggc
    38581 ggcgttctct gccagccatt gtgggaactg acctaacaac tgcacgcgca ccagcgtacc
    38641 agatggtgtc cggctggcgt tttgtagctc agggcaaatg cgatgaaaga cttttaccgg
    38701 cagcagttga tccaccacgc ggacaaactc ggtgatgcac agatcgtagt cgttaacttc
    38761 ggtcagcaat tcacgcacca gagagtcaag cacaccctcc atcggagcca gtaacacacg
    38821 catatcttca cccgcaaaaa aatgaggcgc catgttagcg cctatggtca acggattaaa
    38881 ggtctgcaaa gagggattgc tgcaaactgt aatccctctc tatgcttgat gtatgcctga
    38941 gttcgttgcg cattgcgtaa tgccggggag cgaaaaaata agcgctgatt tcttaatgtg
    39001 atcggtagca cgttttacga attaattgta tgatgaatcc atctcatctg gggtgttgat
    39061 tatgagtaag acactgaaca ttatctggca atatttacgc gctttcgtcc tgatttatgc
    39121 ctgcctgtat gcaggcattt tcattgcttc cctgctaccg gtaaccattc cgggcagcat
    39181 catcggtatg ctgatcctgt ttgtcctgct ggccttgcaa attcttccgg caaaatgggt
    39241 caatccgggg tgctacgtac tgattcgcta tatggcgcta ttgtttgtgc cgattggcgt
    39301 aggcgtcatg caatattttg atttgctccg cgcacagttt ggcccggtag tggtttcctg
    39361 tgcagtcagt acgctggtgg tttttctggt ggtgagctgg agttcacaac tggtacacgg
    39421 tgaacgtaaa gtcgtaggtc agaaaggatc agaagaatga tggcgaatat ctggtggtca
    39481 ttaccgctga cgttgattgt cttttttgcc gcccgcaaac tggcggcacg gtataagttc
    39541 cccctgctta acccgttgct ggtagcaatg gtggtgatca ttcctttttt aatgctcacc
    39601 ggcatctctt acgacagcta ctttaaaggt agcgaagtgc taaacgattt gctgcaaccg
    39661 gcggttgtcg cgttggctta tcctttatat gagcagctac accagatccg cgcgcgctgg
    39721 aaatcgatca tcaccatctg ttttatcggc agcgtggttg cgatggtgac ggggacttcc
    39781 gtggcattgt tgatgggcgc ttcaccggaa atcgccgcgt caatcctgcc gaaatcagtc
    39841 accacgccta ttgcaatggc ggttggcggc agtattggcg gtattccggc aatcagcgca
    39901 gtttgcgtga ttttcgtcgg catcctcggc gcggtatttg gccatacatt gcttaatgcg
    39961 atgcgtattc gtaccaaagc tgcgcgcggt ctggcaatgg ggactgcctc gcacgccctc
    40021 ggtacggcgc gctgcgccga gctggattat caggaaggtg catttagttc gctagcgctg
    40081 gtgttatgcg ggataattac ttcgctgatc gcaccgttcc ttttcccgat tattctggca
    40141 gtaatgggct aaaatttgcg atgcgtcgcg catttttgat gtaagtttca cgcgttgcat
    40201 aattaatgag attcagatca catataaagc cataacgggt tcgtaaactg ttatcccatt
    40261 acatgattat gaggcaacgc catgcatcca cgttttcaaa ccgcttttgc ccaacttgcg
    40321 gataacttgc aatctgcact ggaacctatt ctggcagaca agtacttccc cgctttgttg
    40381 accggggagc aagtctcatc gctgaagagc gcaacggggc tggacgaaga cgcgctggca
    40441 ttcgcactac ttccgctggc ggcggcctgt gcgcgtacgc cattgtcgaa ttttaatgtt
    40501 ggcgcaattg cgcgcggtgt gagcggaacc tggtatttcg gtgccaatat ggaatttatt
    40561 ggtgcgacaa tgcagcaaac cgttcatgcc gaacaaagcg cgatcagcca cgcctggttg
    40621 agtggtgaaa aagcgcttgc agccatcacc gttaactaca cgccttgtgg tcactgccgt
    40681 cagtttatga atgaactgaa cagcggtctg gatctgcgta ttcatctgcc gggccgcgag
    40741 gcacacgcgc tgcgtgacta tctgccggat gcctttgggc cgaaagatct ggagattaaa
    40801 acgctgctga tggacgagca ggatcacggc tatgcgctga ctggtgatgc gctttctcag
    40861 gcggcgattg cagcggcaaa ccgttcgcac atgccttaca gtaagtcgcc aagcggtgtc
    40921 gcgctggaat gtaaagacgg tcgtattttc agcggcagct acgctgaaaa cgccgcattc
    40981 aacccgactc tgccaccgtt gcagggagcg ttaattctat tgaatctcaa gggttatgat
    41041 tacccggata tccagcgcgc ggttctggca gaaaaagccg atgcgccgtt gattcagtgg
    41101 gatgccacct ccgcaacact gaaagctctc ggctgtcaca gtatcgaccg cgtacttctc
    41161 gcttaagcct ggtgccggat gcggcgtgaa cgccttgtcc ggcttgccag ccctctcctg
    41221 gtgtcgaaaa tcccggcaaa cagtttgccg tttcttgcgc aaaaccagcg ggtaaagtag
    41281 cctgatggaa attttcctta gatcgagtct cctgcatgtt aaagcgcgtg ttcctcagcc
    41341 tgttagtcct gatcggcttg ctgctgttga ctgtgctcgg cctcgatcgc tggatgagct
    41401 ggaaaaccgc gccttatatc tacgacgaat tgcaggatct cccctaccgc caggtcggtg
    41461 tggtgctcgg aacagcaaaa tattatcgta ctggcgtaat taatcagtat tatcgctacc
    41521 gcattcaagg agcgattaat gcctataaca gcggtaaggt aaattatcta ttactgagcg
    41581 gcgataacgc attgcaaagt tataatgagc cgatgaccat gcgcaaagat ttaatcgctg
    41641 ctggtgtcga cccatcagat attgttctcg attacgcagg ctttcgtacg ctggattcca
    41701 tcgtgcgtac acgcaaagtt tttgatacta atgatttcat tattatcacc caacgtttcc
    41761 actgtgagcg ggcattattt attgcgctgc atatggggat tcaggctcag tgttatgccg
    41821 taccgtcacc gaaagatatg ctgtcagtac gtattcgcga atttgccgcc cgtttcggtg
    41881 cgctggctga cctttatatt tttaaacgtg aaccgcggtt tttagggccg ctggtcccta
    41941 ttccggctat gcaccaggtg ccggaagatg cgcaggggta tcccgccgtc acacccgaac
    42001 agttacttga attacaaaag aaacaaggaa agtagttatg gatgtacagc agttttttgt
    42061 cgttgccgtt tttttcctta ttccgatatt ttgtttccgc gaagcatgga aaggctggcg
    42121 cgcaggcgcg attgataaac gggttaaaaa tgcaccggaa ccggtgtatg tctggcgagc
    42181 aaaaaatccc ggactctttt tcgcttatat ggtggcatat atcggcttcg gaattttatc
    42241 tatcggcatg attgtttatc ttattttcta tcgttaatat tttctctttt ctgttaatta
//
LOCUS       DAZGSP010000038        41935 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZGSP010000038 DAZGSP010000000
VERSION     DAZGSP010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.038"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..932)
                     /gene="tuf"
                     /locus_tag="ACHK74_002163"
     CDS             complement(<1..932)
                     /gene="tuf"
                     /locus_tag="ACHK74_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP0220981.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVG
                     VLLRGIKREEIERGQVLAKPGTIKPHTKFESEVY"
     gene            complement(1003..3117)
                     /gene="fusA"
                     /locus_tag="ACHK74_002164"
     CDS             complement(1003..3117)
                     /gene="fusA"
                     /locus_tag="ACHK74_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFP0220982.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(3214..3684)
                     /gene="rpsG"
                     /locus_tag="ACHK74_002165"
     CDS             complement(3214..3684)
                     /gene="rpsG"
                     /locus_tag="ACHK74_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFP0220983.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3781..4155)
                     /gene="rpsL"
                     /locus_tag="ACHK74_002166"
     CDS             complement(3781..4155)
                     /gene="rpsL"
                     /locus_tag="ACHK74_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFP0220984.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(4281..4568)
                     /gene="tusB"
                     /locus_tag="ACHK74_002167"
     CDS             complement(4281..4568)
                     /gene="tusB"
                     /locus_tag="ACHK74_002167"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFP0220985.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4576..4935)
                     /gene="tusC"
                     /locus_tag="ACHK74_002168"
     CDS             complement(4576..4935)
                     /gene="tusC"
                     /locus_tag="ACHK74_002168"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFP0220986.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDQQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4935..5321)
                     /gene="tusD"
                     /locus_tag="ACHK74_002169"
     CDS             complement(4935..5321)
                     /gene="tusD"
                     /locus_tag="ACHK74_002169"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFP0220987.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(5321..6043)
                     /gene="yheO"
                     /locus_tag="ACHK74_002170"
     CDS             complement(5321..6043)
                     /gene="yheO"
                     /locus_tag="ACHK74_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0220988.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(6210..7022)
                     /gene="fkpA"
                     /locus_tag="ACHK74_002171"
     CDS             complement(6210..7022)
                     /gene="fkpA"
                     /locus_tag="ACHK74_002171"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP0220989.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            7243..7461
                     /gene="slyX"
                     /locus_tag="ACHK74_002172"
     CDS             7243..7461
                     /gene="slyX"
                     /locus_tag="ACHK74_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFP0220990.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7510..8100)
                     /gene="slyD"
                     /locus_tag="ACHK74_002173"
     CDS             complement(7510..8100)
                     /gene="slyD"
                     /locus_tag="ACHK74_002173"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP0220991.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHQH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(8195..8395)
                     /gene="yheV"
                     /locus_tag="ACHK74_002174"
     CDS             complement(8195..8395)
                     /gene="yheV"
                     /locus_tag="ACHK74_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFP0220992.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8405..10210)
                     /gene="kefB"
                     /locus_tag="ACHK74_002175"
     CDS             complement(8405..10210)
                     /gene="kefB"
                     /locus_tag="ACHK74_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFP0220993.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(10210..10761)
                     /gene="kefG"
                     /locus_tag="ACHK74_002176"
     CDS             complement(10210..10761)
                     /gene="kefG"
                     /locus_tag="ACHK74_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFP0220994.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10892..12805
                     /gene="yheS"
                     /locus_tag="ACHK74_002177"
     CDS             10892..12805
                     /gene="yheS"
                     /locus_tag="ACHK74_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0220995.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12805..13827
                     /gene="yheT"
                     /locus_tag="ACHK74_002178"
     CDS             12805..13827
                     /gene="yheT"
                     /locus_tag="ACHK74_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP0220996.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRRLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13821..14039
                     /gene="yheU"
                     /locus_tag="ACHK74_002179"
     CDS             13821..14039
                     /gene="yheU"
                     /locus_tag="ACHK74_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFP0220997.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            14093..14962
                     /gene="prkB"
                     /locus_tag="ACHK74_002180"
     CDS             14093..14962
                     /gene="prkB"
                     /locus_tag="ACHK74_002180"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFP0220998.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(15017..15421)
                     /gene="yhfA"
                     /locus_tag="ACHK74_002181"
     CDS             complement(15017..15421)
                     /gene="yhfA"
                     /locus_tag="ACHK74_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFP0220999.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15723..16355
                     /gene="crp"
                     /locus_tag="ACHK74_002182"
     CDS             15723..16355
                     /gene="crp"
                     /locus_tag="ACHK74_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFP0221000.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16406..18496
                     /gene="yhfK"
                     /locus_tag="ACHK74_002183"
     CDS             16406..18496
                     /gene="yhfK"
                     /locus_tag="ACHK74_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFP0221001.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18563..19783)
                     /gene="argD"
                     /locus_tag="ACHK74_002184"
                     /gene_synonym="dapC"
     CDS             complement(18563..19783)
                     /gene="argD"
                     /locus_tag="ACHK74_002184"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFP0221002.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCVVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19869..20432)
                     /gene="pabA"
                     /locus_tag="ACHK74_002185"
     CDS             complement(19869..20432)
                     /gene="pabA"
                     /locus_tag="ACHK74_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFP0221003.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20464..21066)
                     /gene="fic"
                     /locus_tag="ACHK74_002186"
     CDS             complement(20464..21066)
                     /gene="fic"
                     /locus_tag="ACHK74_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFP0221004.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(21056..21223)
                     /gene="yhfG"
                     /locus_tag="ACHK74_002187"
     CDS             complement(21056..21223)
                     /gene="yhfG"
                     /locus_tag="ACHK74_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFP0221005.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(21328..21900)
                     /gene="ppiA"
                     /locus_tag="ACHK74_002188"
                     /gene_synonym="rotA"
     CDS             complement(21328..21900)
                     /gene="ppiA"
                     /locus_tag="ACHK74_002188"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFP0221006.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            22171..23352
                     /gene="tsgA"
                     /locus_tag="ACHK74_002189"
     CDS             22171..23352
                     /gene="tsgA"
                     /locus_tag="ACHK74_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFP0221007.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDARVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23614..26157
                     /gene="nirB"
                     /locus_tag="ACHK74_002190"
     CDS             23614..26157
                     /gene="nirB"
                     /locus_tag="ACHK74_002190"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFP0221008.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYSWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            26154..26480
                     /gene="nirD"
                     /locus_tag="ACHK74_002191"
     CDS             26154..26480
                     /gene="nirD"
                     /locus_tag="ACHK74_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFP0221009.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26606..27412
                     /gene="nirC"
                     /locus_tag="ACHK74_002192"
     CDS             26606..27412
                     /gene="nirC"
                     /locus_tag="ACHK74_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFP0221010.1"
                     /translation="MFTDTINKCAANAARIERLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27431..28804
                     /gene="cysG"
                     /locus_tag="ACHK74_002193"
     CDS             27431..28804
                     /gene="cysG"
                     /locus_tag="ACHK74_002193"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFP0221011.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            29051..29218
                     /gene="yhfL"
                     /locus_tag="ACHK74_002194"
     CDS             29051..29218
                     /gene="yhfL"
                     /locus_tag="ACHK74_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFP0221012.1"
                     /translation="MNKFIKVALVGAVLATLTACTDHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            29515..30852
                     /gene="frlA"
                     /locus_tag="ACHK74_002195"
     CDS             29515..30852
                     /gene="frlA"
                     /locus_tag="ACHK74_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFP0221013.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFASPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPNVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            30873..31895
                     /gene="frlB"
                     /locus_tag="ACHK74_002196"
     CDS             30873..31895
                     /gene="frlB"
                     /locus_tag="ACHK74_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFP0221014.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKLLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPDVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYTEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31945..32775
                     /gene="frlC"
                     /locus_tag="ACHK74_002197"
     CDS             31945..32775
                     /gene="frlC"
                     /locus_tag="ACHK74_002197"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFP0221015.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYMLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32772..33557
                     /gene="frlD"
                     /locus_tag="ACHK74_002198"
     CDS             32772..33557
                     /gene="frlD"
                     /locus_tag="ACHK74_002198"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFP0221016.1"
                     /translation="MKPLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAQTIQYHGAW"
     gene            33657..34388
                     /gene="frlR"
                     /locus_tag="ACHK74_002199"
                     /gene_synonym="yhfR"
     CDS             33657..34388
                     /gene="frlR"
                     /locus_tag="ACHK74_002199"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFP0221017.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERISAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(34540..35625)
                     /gene="yhfS"
                     /locus_tag="ACHK74_002200"
     CDS             complement(34540..35625)
                     /gene="yhfS"
                     /locus_tag="ACHK74_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP0221018.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGAEFLTSGDLGLTP
                     GLNQPCITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAASVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35637..36941)
                     /gene="yhfT"
                     /locus_tag="ACHK74_002201"
     CDS             complement(35637..36941)
                     /gene="yhfT"
                     /locus_tag="ACHK74_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFP0221019.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36953..37306)
                     /gene="yhfU"
                     /locus_tag="ACHK74_002202"
     CDS             complement(36953..37306)
                     /gene="yhfU"
                     /locus_tag="ACHK74_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFP0221020.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(37317..38195)
                     /gene="php"
                     /locus_tag="ACHK74_002203"
                     /pseudo
     CDS             complement(37317..38195)
                     /gene="php"
                     /locus_tag="ACHK74_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
     gene            complement(38192..39418)
                     /gene="yhfW"
                     /locus_tag="ACHK74_002204"
     CDS             complement(38192..39418)
                     /gene="yhfW"
                     /locus_tag="ACHK74_002204"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP0221021.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDHVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(39418..40581)
                     /gene="yhfX"
                     /locus_tag="ACHK74_002205"
     CDS             complement(39418..40581)
                     /gene="yhfX"
                     /locus_tag="ACHK74_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFP0221022.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRADLPVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQSERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGCYDSLGNSLGA"
     gene            complement(40665..41027)
                     /gene="yhfY"
                     /locus_tag="ACHK74_002206"
     CDS             complement(40665..41027)
                     /gene="yhfY"
                     /locus_tag="ACHK74_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP0221023.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(41044..41934)
                     /gene="yhfZ"
                     /locus_tag="ACHK74_002207"
     CDS             complement(41044..41934)
                     /gene="yhfZ"
                     /locus_tag="ACHK74_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFP0221024.1"
                     /translation="MKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVGLTQAA
                     LKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEGLASGL
                     KAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALELGPHT
                     YVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESLQRIVK
                     GDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLLRAVVD
                     KHALLAHQQRVVSGEQEPSY"
BASE COUNT        10058 a        11459 c        10560 g         9858 t
ORIGIN      
        1 atgtacactt cagattcgaa cttggtgtgc ggcttgatgg tgcccggctt agccagtacc
       61 tgaccacgtt cgatttcttc acgtttgata ccacgcagca gaacacctac gttctcacca
      121 gcacggcctt cgtccagcag tttgcggaac atttcaacgc cagtacaggt agacttctga
      181 gtctctttga taccaacgat ttcaacttct tcaccaactt tgatgatacc gcgttctaca
      241 cgaccggtaa caacggtacc acgaccggag atggagaata cgtcttcgat cggcagcagg
      301 aacggcttgt caatcgcacg ctctggttcc ggaatataag aatccaggaa gccagccagt
      361 tccaggattt tcgcttccca ctctgcgtcg ccttccagcg ctttcagagc agaaccacga
      421 acgatcggag tgtcgtcgcc cgggaagtcg tactgagaca gaagttcacg aacttccatt
      481 tcaaccagtt ccagcagctc ttcgtcatca accatgtcgc atttgttcag gaacacgatg
      541 atgtacggaa cgcctacctg acgacccagc aggatgtgct cacgagtctg cggcatcggg
      601 ccgtcagtcg cagcaactac caggatcgcg ccgtccatct gagcagcacc ggtgatcatg
      661 tttttaacat agtcggcgtg ccccgggcag tctacgtgtg cgtagtgacg ggtcggggtg
      721 tcatattcaa cgtgagaagt gttgatggtg ataccacgag ctttttcttc cggcgcgtta
      781 tcgatctggt cgaatgcacg agcagcaccg ccgtaggttt tagccagtac ggtggtgatt
      841 gcagcggtca gagtagtttt accgtggtca acgtggccga tagtaccaac gttaacgtgc
      901 ggttttgtac gttcaaattt ttctttagac acggctatat tccttactat agtgctctcc
      961 ccttcaggag agagcacggg actttggtat taacccttag gcttatttac cacgggcttc
     1021 aattacggcc tgagcaacgt tactcggcgc ttcatcatac ttcaggaatt ccatagtgta
     1081 tgatgcacga cctttggtca gagaacgcag ctgagttgcg tatccgaaca tttcagacag
     1141 cggtacttca gcgtggatct taacgccagt aacttcagat tcctgacctt tgagcatacc
     1201 acgacgacgg ctcaagtcac cgataacgtc accggtgttc tcttccggag tttctacttc
     1261 aaccttcatg atcggctcaa gcagaactgg tttcgctttc ttaaagcctt ctttaaaggc
     1321 gatagaagca gccagtttaa acgccagttc agaggagtca acgtcatggt aagaaccgaa
     1381 gtgcagacga atacccatgt ctactaccgg gtagcctgcc agcggacctg ctttcagctg
     1441 ttcctggata cctttatcaa cggccgggat gtattcgcca gggattacac cacctttaat
     1501 gtcgttgatg aactcgtagc ctttcgggtt cgaacccggc tccagcgggt acatgtcgat
     1561 aacaacatga ccatactgac cacgaccacc agactgtttc gcgtgtttac cttcaacatc
     1621 ggtaactttc tggcggatag tttcacggta agcaacctgc ggtttaccta cgttcgcttc
     1681 aacgttgaat tcacgcttca tacggtcaac gatgatgtcg aggtgcagtt cgcccatacc
     1741 cgcgatgatg gtctggttag attcttcgtc agtccataca cggaaagacg ggtcttcttt
     1801 agccagacgg cccagagcca gacccatttt ttcctggtca gctttggttt tcggttcaac
     1861 tgcgatggag attaccggct cagggaattc catacgttcc agaatgatcg gcgcatccgg
     1921 gtcacacagg gtgtcaccag tggttacgtc tttcagaccg atagcagcag cgatgtcgcc
     1981 cgcgcgaact tctttgatct cttcacgttt gttagcgtgc atctgaacga tacgaccgaa
     2041 acgctcacgt gcagctttca cggagttcag tacggtatca ccagagttaa ccacaccgga
     2101 gtaaacacgg aagaaggtca ggttaccaac aaacgggtcg gtagcgattt tgaacgccag
     2161 tgcagagaac ggctcgtcat cacttgcgtg acgttcagcc ggagtgtctt taccgtcgtc
     2221 caggataccg ttgatcgcag gtacgtcaac cggggatggc aggtaatcaa ttaccgcatc
     2281 cagcatcgcc tgaacacctt tgttcttgaa cgcagaacca caggttacca ggatgatttc
     2341 gttgttcaga acgcgctgac gcagagcacc tttgatttct gcttcagtca gttcttcacc
     2401 acccaggtat ttttccatca gctcttcaga agcttcagct gcggattcga tcaggttctg
     2461 gtgccattcg ttagccagtt caaccatgtc tgccgggata tcttcgtatt cgaaggttac
     2521 gccctggtca gcgtcgttcc agttgatagc tttcattttc accaggtcaa caacaccggt
     2581 gaaatgttct tcagcaccaa tcgccagctg cagcggaacc gggttcgcgc ccagacgggt
     2641 tttgatctgg ttaacaactt tcaggaagtt cgcacccatg cggtccattt tgttaacgaa
     2701 cgcaatgcgc ggaactttat atttgtttgc ctgacgccat acggtttcag actgcggctg
     2761 aacaccacca actgcgcagt aaaccattac cgcaccatcg agaacacgca tggaacgttc
     2821 tacttcgatt gtgaagtcaa cgtgccccgg ggtgtcgatg atgttgatgc gatgcggctc
     2881 atactgctta gccataccag accagaatgc agtagtcgca gcggaagtga tggtaatacc
     2941 acgttcctgc tcctgctcca tccagtccat ggttgcagcg ccgtcatgaa cttcaccgat
     3001 tttatggttt acaccggtgt agaacagaat acgttcggta gtagtggttt taccggcgtc
     3061 gatgtgcgca ctgataccga tgttacggta gcgtgcgatg ggtgttgtac gagccatttg
     3121 tttcctcgtt tatcttttag gcgttcaatt taagtagccc aaagcgggct gcttactgga
     3181 agcgcccgcc tggtgactaa aactccgaag ggattaccaa cggtagtgtg cgaacgcctt
     3241 gttggcttcg gccatacggt gaacgtcttc acgtttctta actgcagtac ctttgttttc
     3301 tgcagcatca gaaagttcgt tcgccaggcg cagagccatg gatttatcac cgcgtttacg
     3361 agcagcttca acgatccaac gcattgccag agcattacga cgaaccggac ggacttcaac
     3421 tggtacctga taagtagaac caccaacgcg gcgagactta acttctacag tcgggcgcac
     3481 gttttcgaga gctacttcga atgcttccag ttcagattta ccagagcgct gagccagggt
     3541 ctccagcgcg ctgtatacga tagattcagc agtagatttt ttaccatcta ccatcaggat
     3601 atttacaaat ttagccagca gttctgatcc gaacttcgga tccggcagaa ttttacgctg
     3661 accaatgacg cgacgacgtg gcatggaaat actccgttgt taattcagga ttgtccaaaa
     3721 ctctacgagt ttagtttgac atttaagtta aaacgtttgg ccttacttaa cggagaacca
     3781 ttaagcctta ggacgcttca cgccatactt ggaacgagcc tgcttacggt ctttaacgcc
     3841 ggagcagtca agcgcaccac gtacggtgtg gtaacgaaca cccgggaggt ctttaacacg
     3901 accgccacgg atcaggatca cggagtgctc ctgcaggttg tgaccttcac caccgatgta
     3961 ggaagtcact tcgaaaccgt tagtcagacg aacacggcat actttacgca gcgcggagtt
     4021 cggtttttta ggagtggtag tatatacacg agtacatacg ccacgttttt gcgggcatgc
     4081 ttccagcgca ggcacgttgc ttttcgcaac tttgcgagca cgtggtttgc gtaccagctg
     4141 gttaactgtt gccattaaat agctcctggt tttagctttt gcttcgtaaa cacgtaataa
     4201 aacgtcctca cacaatatga ggacgccgaa ttttagggcg atgccgaaaa ggtgtcaaga
     4261 aatatacaac gatcccgcca tcaccaggcc atctggctgg agtgcttaac cgtaagtctg
     4321 acgaaatcag tatagtcaat gggaatgatg tcgttcgaaa tttgaccagt caaaccgcgg
     4381 gcaataaggt cttcgttcag ggcatagacc ttaatggggg cattacgcag actttcaagg
     4441 tagcggttac cgtcaactgc ggcagttacg ccatcttgca ataatagcag ttcgtcgcct
     4501 tcactgagca gacgcagcag cgcagcaaaa tccgtcagcc agggtgaacg atgtaatgtg
     4561 tgcagcataa acgcctcaaa acctcaaaat aacatcgtag ttggcgagtt cgcggcgtaa
     4621 ggcatctgct tcgagcggcg tggcttcgac aacaaagggg gtctgctgat ctaacccgcg
     4681 ttcgcgcagt gaagccgcac aaacccagca ctgttcaatg tcgtacagac ccaacaattt
     4741 aaaagtggca atgtaatcac gcgccagcac tgcatcgggc ttttgtcctg gcagcagctg
     4801 aaaaacgcca tcagctataa agaagacagc cagatcatca gttaatgcgg aagttgccag
     4861 taaagcatct aaaccttccc gaccagcagc tgtaccatga ggcgcagtag aaaaaacaaa
     4921 cgcaattcgt ttcatcagaa ctgtaccacc ctgtcacagg tcagcgaggc ttccgccagc
     4981 gcgccaagtc cgcttaaggt aaatcccggc tgaaggtttg acgaagccag ccccagtctt
     5041 ccggcctccg tttcatcaac aacgccacgg cgtaatgctg ccgctacgca gatattcagc
     5101 gccacaccat gttgcgcatt caactgttgc cagccgcgca caaggtcaaa ttcgtcgctt
     5161 gccggtgagg tcaattggtt agcgttatag accccttccc gataaaaaaa gacgctgctt
     5221 aactcatggc cttctactat cagcgcctgc gcaaactgaa aagcactact cgcctgttgc
     5281 gtaccgtatg ctggcccggt caccacgatg gcaaaacgca ttacttatct tgcccctgga
     5341 aatcaccgct tttgaactgg cggatgtaga gatagacagt gtgtttggag atgttcaggc
     5401 ggtcagcaac ctggttgatc gcgtctttaa tatcgaagat ccctttctcg tagagattca
     5461 gcacgatctg acgatttttg gcgttattag aaacattgcg atcggcattt acttcttcga
     5521 tggtgaactc cagcgtttgg gtaaccagat cttcaacaga agaggcaaag ttgacgcttg
     5581 aaccgacatc cggggtttct ggcggcacaa aagtgctcat aatctgcgag aagggaacat
     5641 caagattcat attgatgcac agcagaccaa ttacacgctg ttcgcggtta cgaatcgcga
     5701 tagtcaggga cttcattaat acgccgcttt tggcgcgagt aaagtagcat ttagaaacgc
     5761 tgctatccgc tcccgtcata tcgtgcagca tacgtagcgc caggtcagta attggcgaac
     5821 caatcttccg gcctgtatgt tcaccgttag caatgcgaat ggctgaacat tttagatcct
     5881 gcaaagagtg caaaacgatt tcacagtggg agccaataag catcgctaac ccgtccacca
     5941 ccgcttcgta ggatttcaga atatcaaaat cggtctggtc gaaaggacgt tgatccagta
     6001 aatccaactc actggtttcg ttggttaaaa gcgacctgga catgaaaaaa aacactcctt
     6061 ttcaggagcc tgtcgttaac ttttcagggc aggctcatta atgatgcggg taactaaatt
     6121 aatacagcgg aggttccgct ttccagcact aattatatcc ggcctgtaat aaaaaaaccg
     6181 ccgcctggtc aggcggcggt tcttaatgct tattttttag cagaatctgc ggctttcgca
     6241 tcagcttccg gctttgcatc agccttcggc gctggtttca catccagcag ctctacgtca
     6301 aacaccaggg tagaattcgg tgggatcccc ggaacacccg ctttgccgta agccagttct
     6361 ggtggaataa ccagtttgat cttaccgcct ttcttgatgt tcttcagacc ttctgtccac
     6421 cccgggataa caccgtccag acggaaagag agcggttcac cacgggtgta agagttgtcg
     6481 aactctttac cgtcgatcag cgtacctttg tagttcacga caacagtatc gctgtctttc
     6541 ggtgcttcgc ctttaccggc ttctactacc tgataaacca gaccagttga agaggttttc
     6601 acacctttct ctttggcaaa tttctcgcgg tactctttac cttttgcttc gttatcagcc
     6661 gcgtcttttt ccatcttcgc ctgagcagaa gacttcacgc gagcttcgaa tgcttgcaga
     6721 gtctgttcga tctcttggtc ggagagtttg ctcttatcag caaatgcatc ctgaacacca
     6781 gcgatcagct gatctttatc cagtttgatg cccagttttt cttgttcttt aagagagttt
     6841 tccatgtaac gacccagcga agcacccagt gcataagctg atttctgatc gtcatttttg
     6901 aacgctgctt tgctgtcagc agttgtagca ggttttgcag cttcagcagc aaaagtgatt
     6961 ggtgcatgca gggcaacggc cattgtggtc gccagcagcg ttactttaaa cagtgatttc
     7021 atccatatct ccaggatcgg ggcatctcac cccagggtta actattatca gaagggtact
     7081 ataaagcgtt gtcgaacaaa tctatataca gacacgccct attatcatct attttcagac
     7141 tctttttgtt taaattagtt tcgatgaccg cgaaatgagt gctgtctcgg gcagcaaagt
     7201 taagtagaat ccgcggcgac cattcgacaa aagaggtgaa tcatgcagga tttatcattg
     7261 gaagcacgcc tggctgagct ggagagcaga ctggcttttc aggaaatcac cattgaagaa
     7321 ctgaacgtca cggtgaccgc tcatgaaatg gagatggcga aactgcgcga tcatctgcgt
     7381 ctgctgaccg agaagttaaa agccagccag ccgtcgaaca tcgcgtcgca ggctgaagaa
     7441 acgccaccgc cacattattg aggcgtaaaa aaagcgggga ttccccgctt ttttgtcacc
     7501 ttttcggtat tagtggcaac cacaaccgcc gttgccttta ccgccacagc aaccttcgcc
     7561 accgtgttca tgaccgtgat cgtggccatg accgccgcag caaccgtcgt ggtcgtgatc
     7621 gtgttgatga tcgtgcgcgc cgtgaacgtg gccatgagcc agttcttctt cggtcgcttc
     7681 gcgaatcgcc acaacttcaa cgttgaattt caggttctga ccggccagca tgtggttacc
     7741 atcaaccacg acgtgatcgt cttccaccgc agtgatttca accggtaccg gaccctggtc
     7801 ggtttcagcc aggaaacgca tacctacctg cagttcatca acgcccataa atacgtcttt
     7861 aggcacacgt tgcaccaggt tttcgtcgta ctgaccgtaa gcgtcgttcg cgccaacagc
     7921 gacatcaaat ttgtcgccaa cttcatgacc ttccagcgcc gtttccaggc cagagatcag
     7981 ggaaccgtga ccatgcaggt agtccagcgg cgcactcacc ggagactcat caaccaacac
     8041 accgtcttct gtacgtacct gataggccag gctgaccacc aggtcttttg ctactttcat
     8101 gatatctcct gagcatggga agaatagtgg cgcagattgt agcggaattc tgcagccgtg
     8161 tactcactag cttaaaaaaa ctcggcgcat atcgctagtc cggatgaaaa atcccgatca
     8221 cttgctcatc tttgcgaacg tgatcgcggg cttctttgtc tgcttctcgc atctgatgtc
     8281 cgcacttaac acattcaaca atatcaatat tattttcgcg ccacatcgcc attgaatcct
     8341 gcgcctgaca ggccgggcat tttgcgcccg cgataaaacg ttttcggatt gccatcttta
     8401 ccctctactc aaattcatcc cagccgtcca gctggcgtcg ttcttgttgc atttcacgct
     8461 ggaaaatctc ttccagttcg cgtcgggctt ccctggcgcg agaaatttgt acggtatcag
     8521 catgcatcgg gattaactcc cgcagcattc gcatatccag gcggcgaaaa tgcagttgcg
     8581 cccgctgtgc ctgatgcgga tgcatgccaa gcgtgaccag cgtcttgcgc cccagctcta
     8641 acgcactgga gaatgtttca cgggaaaact gcgtcacccc tgcctgtaat aactcatgcg
     8701 cttccacacg tccgcgcgct cgcgcaagaa tatgcaaatg cggaaagtgc tgctggcata
     8761 tttccaccag cttcatggtg tcttccggct cgttacaggt aatgacgata gactcagcgg
     8821 cctctgcacc cgcagaacgt aaaagatcga cctgcgtggc gtcgccgtaa taaactttgt
     8881 agccatattt acgcatcagg ttaacggcgc tgatatcccg ctccagcacg gtaatgcgca
     8941 ttttatttgc catcagcaaa cgaccaatca cctgaccaaa acgcccgaag cccacgacaa
     9001 tgacctgggg tttatcatcg ttgacccacg gtttttcgtc ttcttcttcc ggtccgttaa
     9061 actggcggga tagccattta tccaccagct tcatcagcaa cggcgtggtc atcatggaaa
     9121 gcgtcaccgt caccagcaac agcgccatct ggtcgccctg gaataaacgt tgtgaagaag
     9181 cggtagaaaa gaggacaaag gcaaactcac cgccctgact caacacgcca gcaaactgca
     9241 tccgctctga gctacgcacg ccatacaatc gcgccagcag atacagcacg agaattttca
     9301 ccgccaccag cacaaccacg cttatcacta cccacaacag atgggtataa agcaccccga
     9361 ggttgagcga catgccgaca gagataaaga acaaaccgag cagcaatcct ttgaagggat
     9421 cgatagccgt ttccagttca tggcgatatt cactttccgc cagcagcacg cccgcaataa
     9481 atgtaccgag cgccatcgac agccccagcg catccataaa caatgcggaa cccaacacca
     9541 gcagcagcgt cgcggcggtg aacacttccc gcacgccaga agctgcaata aagcggaata
     9601 ccggacgcag taaatagcgc ccaccaatca gcatgccgac aaacgccagc accttcatgc
     9661 cgatcttcat ccagtcgaaa tgttcgtctg ccgaacccgc caacaacggc actaacgcca
     9721 gtgccgggat caccgccaga tcctgaaaca gcagtaccga aaacccgagc tgcccggatt
     9781 cgctgcgatt catccctttc tcacgcatca attgcaacgc cattgcggtc gaagacatcg
     9841 caaggccaat gccaccgacc accgccgcct gccaggcgaa atccgtcagc atcaataatc
     9901 ccgccagcaa cgccgcgctt aacagcacct gtgccgcgcc tacgccaaaa atcgaacgcc
     9961 gcagttgcca aagcttggag ggattcaact caaggccgat gataaacatc aggaatacca
    10021 cgccgagttc cgaaaagtgg aggatctcat cgacgtcgct aataaacccc agcccccacg
    10081 ggccaattgc aatccctgcc agcaaatatc ccaacacagc gccaatcccc agccgcgatg
    10141 ccaacggcac cgcagccacc gccgcgaaga gaaacagcac tcctgcgagt aaaaaatcgg
    10201 aaccttccat cagcggcctc ctggagacag cggatttgcc agccagtcac cgtaggctct
    10261 ggcgtggctc gccagctcct gtgcgctttg ccgtctcgcc cagtaaataa tgatgggact
    10321 taaccaatgc atccggcaca tgcccgccgc cagttcaaag ggacgcagaa cgtcgctcat
    10381 ggggtagcga ttcaatgcgt cataacggta agcactttcc ggctcgccgg tggtgatcac
    10441 gctacgccag tactttcccg ccagttggtt tcctcccggc ccgctggcaa aaccacggct
    10501 taatacccgg tccagccact ctttcagtag cgccgggcag ctataggtat aaagaggatg
    10561 ctgaaagaca atcacctcgt gctcgcgcag taatgcctgc tcacggggga tatcaataaa
    10621 aaaatcggga tagtgcgcat aaaggtcgtg cacggtaaca ttgctgagct gcgtagccgg
    10681 tttaagcagt acccggtttg ccaccgagtc ctgagattcc ggatgggcat acagcagcaa
    10741 aactttcgct ggctgagaca tcatccccct cccggtatgg tttttgtgta tagtcgctgt
    10801 tttgggctac cattgcgccc gatgcggcag ctcgcccata cattacatta tcataatgat
    10861 aagttaacat agtctgaaca tacggcacct tatgattgtt ttctcctcgt tacaaattcg
    10921 tcgcggcgtg cgcgtcctgc tggataatgc caccgccacc atcaaccctg ggcagaaagt
    10981 cggcctggtg ggtaaaaacg gctgtggtaa atctaccctg ctggcattgc tgaaaaatga
    11041 aatcagcgcc gacggcggca gctacacctt tccgggaagc tggcaactgg cgtgggtgaa
    11101 tcaggaaacg ccggcgttac cgcaagcggc gctggaatat gtcattgacg gcgaccgtga
    11161 atatcgtcaa ctagaagcgc agctgcacga cgccaacgaa cgtaacgacg ggcacgccat
    11221 tgcgaccatt catggcaagc tggatgctat tgacgcatgg agtattcgct cccgtgccgc
    11281 cagcctgctg cacggcctcg gtttcagcaa tgaacaactg gagcgcccgg taagtgattt
    11341 ctccggtggc tggcgtatgc gtcttaacct tgcccaggcg ctgatttgcc gttcagactt
    11401 gctgctgctc gacgaaccga ctaaccacct cgatctcgat gccgttatct ggctggaaaa
    11461 atggttgaag agctatcaag gcacgctgat cctgatctct cacgaccgcg acttcctcga
    11521 tccgatcgtt gataaaatta ttcatatcga acaacaaagc atgttcgagt acactggcaa
    11581 ctacagttcg tttgaagtac agcgcgccac ccgtctggcg cagcaacaag cgatgtatga
    11641 aagccagcag gaacgcgtgg cacatctgca aagttatatc gaccgtttcc gtgccaaagc
    11701 caccaaagcg aagcaggccc agagccgcat taagatgctt gagcgtatgg agctgattgc
    11761 ccccgcgcac gtcgacaacc cgttccgctt tagcttccgc gcgccggaaa gcctgccaaa
    11821 tccgttactg aagatggaaa aagtcagcgc gggctatggc gatcgcatta ttctcgactc
    11881 gattaaactg aacctggtgc ccggctcgcg cattggtctg ttaggccgca acggcgcggg
    11941 taaatcgaca ttaatcaaac tgttagccgg tgaacttgcg ccagtcagcg gtgaaattgg
    12001 tctggcgaaa gggatcaagc tcggctactt cgcccagcat caacttgaat acctgcgcgc
    12061 cgacgaatca cctattcaac atctggcacg tttagcgccg caggagctgg aacaaaaact
    12121 gcgtgactac ctcggcggct ttggtttcca gggcgataaa gtaaccgaag aaacgcgccg
    12181 cttctccggt ggggaaaaag cccgcctggt gctggcatta attgtctggc agcgtccgaa
    12241 tctgctgctg ctcgacgaac cgactaacca ccttgacctc gacatgcgtc aggcactcac
    12301 cgaagcatta atcgagtttg aaggcgcgct ggttgtcgtt tcgcacgacc gtcatttgct
    12361 gcgttccacc actgacgatc tctacctggt tcacgatcgt aaagtcgaac cgttcgacgg
    12421 cgatctggaa gattatcaac agtggttgag cgacgtacaa aagcaggaaa accagaccga
    12481 cgaagcgcca aaagagaacg cgaacagcgc ccaggcacgt aaagatcaga agcgtcggga
    12541 agcggagctg cgtgcgcaaa cccagccgct gcgtaaagag attgcccgtc tggaaaaaga
    12601 gatggagaag ctgaacgcgc aactggcgca ggcggaagag aaactcggcg acagcgaact
    12661 gtatgaccag agccgtaaag cggagttgac cgcctgcctg caacagcaag ccagcgccaa
    12721 atccggcctg gaagagtgcg aaatggcgtg gctggaagcc caggagcagc ttgagcagat
    12781 gctgctggaa ggccaaagca actgatggcg cagataacga cgaccgatgc caatgaattt
    12841 agcagcagtg ctgaattcac ccctatgcgc ggctttagca attgtcatct acaaactatg
    12901 ctgccgcgtt tgtttcgtcg tcaggtgaaa ttcaccccgt actggcagcg gctggagttg
    12961 cccgacggcg attttgtcga tctcgcgtgg agtgaagacc ccgcacaggc gaaacataaa
    13021 ccgcgtttag tggtgtttca cgggctggaa ggcagtctca atagccctta cgcccacggt
    13081 ctggttgagg cggcgcaaaa gcgcggctgg ctgggcgtgg tgatgcattt tcgcggatgc
    13141 agcggtgaac caaaccgtat gcaccgcatt taccattcgg gcgaaaccga agacgccagt
    13201 tggtttttac gcaggctgca acgcgagttt ggtcatgcgc caacagctgc cgtcggctat
    13261 tcgctcggcg gtaatatgct ggcctgtttg ctggcaaaag aaggcaatga tctcccgatt
    13321 gatgcggcgg tgattgtctc tgcgccgttt atgctggaag cctgtagcta tcatatggaa
    13381 aagggctttt cccgcgttta tcagcgttac ttgctgaacc tgttaaaagc caatgccgca
    13441 cgcaagctgg cagcctaccc cggaacactg ccgattaatc tcgcgcagtt aaaatcggta
    13501 cgtcgcatcc gtgaatttga cgatctaatc accgccagaa ttcacggcta cgccgacgct
    13561 atcgactatt atcgtcagtg tagcgccatg ccgatgctga accggatcgc caaaccgacg
    13621 ctgattattc acgccaaaga cgatccgttt atggatcatc aggtgatccc gaaaccggaa
    13681 agtctccccc cgcaggtgga gtatcaactg actgaacatg gcggtcatgt tggctttatt
    13741 ggcggtacat tacttcatcc gcaaatgtgg ctggagtcac gcattcctga ctggttaaca
    13801 acgtatctgg aggcgaaatc atgttgattc cgtggcaaga cctctccccc gaaacgctgg
    13861 aaaatttaat tgaaagcttt gtgttacgtg aaggcaccga ttatggtgaa catgagcgta
    13921 cacttgaaca gaaagtcgcc gacgtcaaac gccagctaca gtgcggagaa gcggtgctgg
    13981 tatggtcgga gctgcacgaa acggtcaata ttatgccgcg cagtcagttt cgcgaataac
    14041 ctgcaaccgt cggcggacga gataataatg atagctaagt catggagtta ctatgtctgc
    14101 caaacatccg gtcattgcgg taacaggatc cagcggcgcg gggaccacca ccaccagcct
    14161 cgcgtttcgt aaaatattcg cgcagttaaa tctgcatgca gctgaggtgg aaggcgacag
    14221 ttttcaccgt tacacccgcc cggaaatgga catggcgatc cgcaaagcgc gcgacgccgg
    14281 gcggcatatc agctacttcg gccccgaagc gaacgacttc ggcctgctgg aacaaacctt
    14341 cattgaatac ggtcagagcg gcaaagggaa atctcgcaaa tatctgcata cctacgatga
    14401 agccgtaccg tggaatcagg taccgggaac attcaccccc tggcaacctt taccagaacc
    14461 cactgatgta ctgttttatg aaggtttaca cggcggcgta gtcacgccac agcataacgt
    14521 tgcgcagcat gtggacttac tggtcggcgt ggtgcctatc gttaaccttg agtggattca
    14581 aaaactgatc cgcgacacca gcgagcgcgg gcactcacga gaagcagtga tggactcagt
    14641 agtgcgttca atggaagact atatcaacta catcacaccg cagttttccc gcacccatct
    14701 taacttccag cgcgttccca ccgtcgacac ttcaaacccg ttcgcggcaa aaggtatccc
    14761 gtcgctggat gaaagctttg tggtgatcca ttttcgtaat ctggaaggga tcgatttccc
    14821 ctggctgctg gcgatgttgc aaggctcatt catttcccac atcaatacgt tagtggtacc
    14881 gggcggcaaa atggggctgg caatggaatt aattatgctg ccgctggtgc aacgattgat
    14941 ggaaggaaag aaaatcgagt aactctgcta ttacgccgga taaaatacta tccggcttca
    15001 caacgggata gttaaatcac gcggcaacca cttcatacga gtgagtaata ttcaccgctt
    15061 tttccagcat caacgccact gaacaatatt tctcagcaga gagatcaacc gcacgcgcaa
    15121 ccgctgcgtc tttcaggtcg cggccggtga cgataaaatg cagattaatg tgcgtaaaca
    15181 ggcgtggtgc ctcttcgcgg cgttcagagg tcaattttac ttcacaatcg accacatcct
    15241 gacgcccttt ttgcagaata gaaaccacat cgatggcact gcaaccaccc gccgccatca
    15301 acaccatttc catcggactt ggtgctttat cgcctgagtt gccgtccatt aaaatctggt
    15361 gaccagaggc agattcgccc agaaaagtta acccttcgac ccacttcact cgcgcttgca
    15421 tttttgctac tccactgcgt caattttcct gacagagtac gcgtactaac caaatcgcgc
    15481 aacggaaggc gacctgggtc atgctgaagc gagacaccag gagacacaaa gcgaaagcta
    15541 tgctaaaaca gtcaggatgc tacagtaata cattgatgta ctgcatgtat gcaaaggacg
    15601 tcacattacc gtgcagtaca gttgatagcc ccttcccagg tagcgggaag catatttcgg
    15661 caatccagag acagcggcgt tatctggctc tggagaaagc ttataacaga ggataaccgc
    15721 gcatggtgct tggcaaaccg caaacagacc cgactctcga atggttcttg tctcattgcc
    15781 acattcataa gtacccatcc aagagcacgc ttattcacca gggtgaaaaa gcggaaacgc
    15841 tgtactacat cgttaaaggc tctgtggcag tgctgatcaa agacgaagag ggtaaagaaa
    15901 tgatcctctc ctatctgaat cagggtgatt ttattggcga actgggcctg tttgaagagg
    15961 gccaggaacg tagcgcatgg gtacgtgcga aaaccgcctg tgaagtggct gaaatttcgt
    16021 acaaaaaatt tcgccaattg attcaggtaa acccggacat tctgatgcgt ctgtctgcac
    16081 agatggcgcg tcgtctgcaa gtcacttcag agaaagtggg caacctggcg ttcctcgacg
    16141 tgacgggccg cattgcacag actctgctga acctggcaaa acaaccagac gctatgactc
    16201 acccggacgg tatgcaaatc aaaattaccc gtcaggaaat tggtcagatt gtcggctgtt
    16261 ctcgtgaaac cgtgggacgc attttgaaga tgctggaaga tcagaacctg atctccgcac
    16321 acggtaaaac catcgtcgtt tacggcactc gttaatcccg tcggagtggc gcgttacctg
    16381 gtagcgcgcc attttgtttc ccccgatgtg gcgcagactg atttatcacc ccgatatcaa
    16441 ctatgcactg cgacaaacgc tggtgctatg tttgcccgtg gctgttgggt taatgcttgg
    16501 cgaattacga ttcggtctgc tcttctccct cgttcctgcc tgttgcaata ttgcgggcct
    16561 tgatacgcct cataaacgtt ttttcaaacg cttaatcatt ggtgcgtcgc tgtttgccac
    16621 ctgtagcttg ctgacacagc tactactggc aaaagatgtt cccctgccct ttttgctgac
    16681 cggattaacg ctggtacttg gcgtcactgc tgagctgggg ccattgcacg ctaaattgct
    16741 tcccgcatcg ctgctcgccg ccatttttac cctcagtctg gcaggataca tgccggtctg
    16801 ggaaccgttg ctcatctatg cgttgggcac tctctggtac ggattgttta actggttttg
    16861 gttctggatc tggcgcgaac aaccgctgcg cgagtcacta agtctgctgt accgcgaact
    16921 ggcagattat tgtgaagcca aatacagcct gcttacccag cacaccgacc ctgaaaaagc
    16981 gctgccgccg ctgctggtgc gccagcaaaa agcggtcgat ctaattaccc agtgctatca
    17041 gcaaatgcat atgctttccg cgcaaaataa tactgactac aagcggatgc tgcgtatttt
    17101 ccaggaggcg ctggatttac aggaacatat ttcggtcagt ttgcatcagc cggaagaggt
    17161 gcaaaagctg gtcgagcgta gccatgcgga agaagttatc cgctggaatg cgcaaaccgt
    17221 cgccgctcgc ctgcgcgtgc tggctgatga cattctttac catcgcctgc caacgcgttt
    17281 tacgatggaa aagcaaattg gcgcactgga aaaaatcgcc cgccagcatc cggataatcc
    17341 ggttgggcaa ttctgctact ggcatttcag ccgcatcgcc cgcgtgctgc gcacccaaaa
    17401 accgctctat gcccgtgact tactggccga taaacagcgg cgaatgccat tacttccggc
    17461 gctgaaaagt tatctgtcac taaagtctcc ggcgctacgc aatgccggac gactcagtgt
    17521 gatgttaagc gttgccagcc tgatgggcac cgcgctgcat ctgccgaagt cgtactggat
    17581 cctgatgacg gtattgctgg tgacacaaaa tggctatggc gcaacccgtc tgaggattgt
    17641 gaatcgctcc gtgggaaccg tggtcgggtt aatcattgcg ggcgtggcgc tgcactttaa
    17701 aattcccgaa ggttacaccc tgacgttgat gctgattacc accctcgcca gctacctgat
    17761 attgcgcaaa aactacggct gggcgacggt cggttttact attaccgcag tgtataccct
    17821 gcaactattg tggttgaacg gcgagcaata catccttccg cgtcttatcg ataccattat
    17881 tggttgttta attgctttcg gcggtactgt ctggctgtgg ccgcagtggc agagcgggtt
    17941 attgcgtaaa aacgcccatg acgctttaga agcctatcag gaagcgattc gcttgattct
    18001 tagcgaggat ccgcaaccta cgccactggc ctggcagcga atgcgggtaa atcaggcaca
    18061 taacactctg tataactcat tgaatcaggc gatgcaggaa ccggcgttta acagccatta
    18121 tctggcagat atgaaactgt gggtaacgca cagccagttt attgttgagc atattaatgc
    18181 catgaccacg ctggcgcggg aacaccgggc attgccacct gaactggcac aagagtattt
    18241 acagtcttgt gaaatcgcca ttcagcgttg tcagcagcga ctggagtatg acgaaccggg
    18301 tagttctggc gatgccaata tcatggatgc gccggagatg cagccgcacg aaggcgcggc
    18361 aggtacgctg gagcagcatt tacagcgggt tattggtcat ctgaacacca tgcacaccat
    18421 ttcgtcgatg gcatggcgtc agcgaccgca tcacgggatt tggctgagtc gcaagttgcg
    18481 ggattcgaag gcgtaatgca ggctaaacca tcggataagg cattcacgcc gcatccgaca
    18541 tcttttgcct gatgcgtctt atttacgccc caaccacctt cgccaccgcg tgggcgaaac
    18601 gttgcatccc ttcatcgata tccgcatctt ccaccaccag cgacggcgca aaacgcatca
    18661 catccggtcc ggcattcagc accattacgc cagcctctgc gcccgcatac aggaaatcac
    18721 gcgcccgacc tttgtactgt ggtttcagct ccgcgccaat taacagcccc ataccgcgaa
    18781 tatcgctaaa cacatcgtac tgctgatcaa tcttctgcaa atggtcaaca aaacgctggc
    18841 gtttcgcctg aatgccttcc agcacttcag gggtattaat gatatcaaat gccgcccccg
    18901 ctactgcaca ggccagagga ttaccgccgt aggtggaacc gtgagaacca ggatgaaacg
    18961 cagaagcaat ttccgccgtg gtcagcatgg cgctaatcgg gaagccgccg cctaacgctt
    19021 tcgcagaggt cagaatatcc ggcgtcacgc cgtagtgcat gtaagcaaac aaatcgccgg
    19081 tccgccccat cccgcactgt acttcatcaa acaccaataa tgcctgatgt tgatcgcaca
    19141 gctcgcgcaa gccctgcaaa aactctggcg tcgctgccgt cacgccgccc tcgccctgga
    19201 tcggctcaac caccaccaca caggtgtgat catccatcac cgctttcact gcgtggagat
    19261 cgttaaaggg aacgtggatg atgtctgccg gtttcggccc aaagccgtcg gaatattttg
    19321 gctgcccacc caccgaaacg gtaaacagcg agcgaccatg aaaagcgtta tggaaggcaa
    19381 taattttggt tttgaacggg ctatgacgca cacaggcgta atggcgtgcc agtttaaagg
    19441 cggtttcgtt agcttccgtg ccggagttca taaacaccac gcgttcggca aacgtcgcct
    19501 caatcagttt gcgcccaaga cgcagcgccg gttcattggt gaaaacgtta ctgatatgcc
    19561 acagagtttc gccctgggtt tttaacgcgt tcaccagcgc aggatggcaa tggcccaacg
    19621 ccgtaactgc aatgccaccc gcgaaatcga catactcctt gccttgctga tcccagattc
    19681 ggctgccctg accttttacc ggaataaact ctgccggagc ataaatcggc aggatcactt
    19741 catcgaaagt cgcgcgtgta attgctgttt gttcaattgc catctcatga tcaccctgtt
    19801 acgcataaat aaatatgaaa ttataatcac aaaatatgca taaaaaatca ctgaatggca
    19861 atcagaaatc agcgatgcag gaaattagcc agcagttgat gtccttgttc gctaagaata
    19921 ctttctggat ggaactgcac accttccaga tcccactggc gatggcgaat ccccataatc
    19981 tcgcgggttt cgctccaggc cgtcacgtca aagcacgctg gtaatgagcc aggttccacc
    20041 accagcgaat ggtagcgtgt cacggtaagt ggatttgcca gcccccgaaa tacgccctca
    20101 ccgttatgtg taatcggcga ggttttgccg tgcatgacct ttgcggcgcg cacaacttta
    20161 ccgccaaatg cctgcgccat tgcctgatga ccgaggcaga cgccaagaat cggcaagcgc
    20221 ccggcatagt ggcgaataac gtcaagggag atcccggctt catctggcgt acaggggcca
    20281 ggtgagatga caattttttg tggtttaagg gcgtcgatat ccgccagcgt caacgcatcg
    20341 ttgcgcttaa ccagcacatc cgcccccagt tcacaaaagt actggtagag gttccaggta
    20401 aaagaatcgt agttatctat aagcaggatc atggcggctc cggtacgaaa gaaccgcgct
    20461 attttactca gattccccgg cttcgcttac cactttgcta aatatcatct gcagtgcggt
    20521 aagatccccc attgccccac tctgatttgc ctgattccag gcctcttttt cgataccctg
    20581 ccagctcagt tgatatccgg catgaatcgc cagttgctcg aagaagatcc gctgtgccag
    20641 accacttccc acccggaagg gatgcagcac gttgatttca caatagtaat gcgccagccg
    20701 ctcgacgaac ttcgctttct ccaggccaac cagataacct tcttcctcta gatcctgcat
    20761 cagggcattg ccctcttttt cgatataagc aaagtggcag aacggcgtat cgccctgata
    20821 aatatcaact tcacgcagtt gccccgccca gtcgaaaata tcctgataca gctggcgatg
    20881 gatagtgcgc aaatgcggta aaccgcgcac cagcggacca agctcaatgg tcgcagcacg
    20941 cagcgccgtc atttcgtaag cggcctgttc cagccgctgt tgctggcgga tgttcagccg
    21001 gttacgcatg atatcaaggc caggataaag atacggatcg cgcccttcgc cgaatttatc
    21061 gctcatagtg cctcctcagc tcttcaaggc gcgctaaagc ctctgcggca gtaagagtga
    21121 ctaaaggcat ctcgacgcct tcaagacggc gactggcctg gaaattacga ttacgctgaa
    21181 gctcccagag acgggacttt tgcttatcgg tgagtttctt cacttgatgc ctccctgaac
    21241 gtgtccattt gccacaagta taagcggcaa atccgggtta cgccgggaga gcagaagcgc
    21301 gggcaagatt gcccgcgcga gaaatcatta cggcaggact ttagcggaaa ggataactac
    21361 cggttttgac ggcacattct ggtacggacc aacgtcatga gtcggcacct gggaaatctt
    21421 atcggcaacg tccatgcctt tcaccacttt accaaatacc gcgtaaccga aatcacgctg
    21481 accatggtca aggaaggcgt tatcggcaac gttgataaag aactggctgg tggcgctgtc
    21541 tttgtcagcg gtacgcgcca tcgcgatggt gccacgcgtg ttgcgcaggc cgttatcggc
    21601 ttcatttttg attggcgggt ttggtttttt ctgctgcatc tgctcggtga aaccgccgcc
    21661 ctgaatcata aagccaggaa tgacgcggtg aaaggtagtg ttgttataaa aaccgctgtt
    21721 cacataatcg acaaagtttt gcacagacac tggcgctttt tgtttatcca gctccagttc
    21781 gatgttacca gctgaggttg tcaacaatac gtgcgggtcc cctttcgctg ccattgctgc
    21841 gggagaaaga gcagaaagag cgaaaacagc agccatcgcc gccagggtcg atttgaacat
    21901 gagatttcct taacaaacag agcagaaaaa aagcaagtga agagattcta aatagccttc
    21961 aagaacaagg ccatcccttt acctaatttt acgtatctga aatatatgta acctgacgat
    22021 cactttttat tccgtgatca aaatcacctc ttaaaatgca atttagcaac cgattgcaat
    22081 aaaacattta aacagatcac aaaatcacct aaaatcgccc gctcgcggca tatcccgcta
    22141 tgccatttta tttttgaacg caggccaatc atgactaaca gcaatcgcat caagctcaca
    22201 tggattagct ttctctctta cgcactgacc ggtgcgttgg ttattgtcac cgggatggtg
    22261 atgggaaata tcgccgatta tttcaatctg ccggtttcca gtatgagtaa caccttcacc
    22321 ttcctcaacg ccggcatttt aatctctatc ttcctcaacg catggctgat ggaaattgtc
    22381 ccgttgaaaa cgcagttacg ttttggcttt ctcctgatgg tgctggcggt tgccggtttg
    22441 atgttcagcc acagcctggc gctgttctcg gcggcgatgt tcattctcgg ggtggtcagt
    22501 ggcatcacca tgtcgattgg tacattcctg gtaacacaaa tgtatgaagg gcgtcagcgc
    22561 ggttcccgcc tgttatttac cgactccttc ttcagtatgg ctgggatgat tttcccaatg
    22621 atcgccgcgt ttctactggc gcgcagcatt gagtggtact gggtttatgc ctgcatcggg
    22681 ctggtgtatg tcgctatttt tattctgacc ttcggctgtg agttcccggc actgggcaaa
    22741 catgcaccaa aaacggatgc tcgggtagcg aaagaaaagt gggggatcgg cgtactgttt
    22801 ctctctgttg cggcgctgtg ctacatcctc ggtcagttag gttttatctc ctgggtgcct
    22861 gagtatgcca aaggcctggg catgagcctg aatgatgctg gcacgctggt gagtaacttc
    22921 tggatgtcat acatggtcgg tatgtgggcg ttcagcttta ttcttcgctt ctttgatttg
    22981 caacgcattc tgaccgtact ggctggtctg gctgcgattc tgatgtacgt ctttaacacc
    23041 ggaacaccag cacatatggc gtggtcaatt ctcgctctgg gcttcttctc cagcgcgatc
    23101 tataccacca tcatcaccct gggttcacag cagaccaaag taccgtcgcc aaaactggtt
    23161 aactttgtcc tgacctgcgg aaccatcggt actatgttga cctttgtggt taccggcccg
    23221 attgttgaac atagcggtcc gcaggcggca ctgcttacag caaacggtct gtacgctgtc
    23281 gtctttgtga tgtgcttcct gttaggtttc gtcagccgtc accgtcagca taacaccctg
    23341 acctctcatt aattgctcat gccggacggc actatcgtcg tccggccttt tcctctctta
    23401 ccccgctacg tgcatctatt tctataaacc cgttcatttt gtctattttt tgcacaaaca
    23461 tgaaatgtca gacaattccg tgacttaaga aaatttatac aaatcagcaa tatacccctt
    23521 aaggagtata taaaggtgaa tttgatttac atcaataagc ggggttgctg aatcgttaag
    23581 gtaggcggta atagaaaaga aatcgaggca aaaatgagca aagtcagact cgcaattatc
    23641 ggtaacggta tggtcggcca tcgctttatc gaagatcttc ttgataaatc tgatgcggcc
    23701 aactttgata ttaccgtttt ctgtgaagaa ccgcgcatcg cttatgaccg cgtacacctc
    23761 tcgtcttact tctctcacca caccgccgaa gagctgtcgc tggtgcgcga aggcttctac
    23821 gagaaacacg gcatcaaagt tctggtcggc gaacgcgcta tcaccatcaa ccgtcaggag
    23881 aaggtgattc actccagcgc cgggcgcacc gttttttatg acaagctgat catggcgacc
    23941 ggttcctact cgtggatccc gccaatcaaa ggttctgata ctcaggactg ctttgtctat
    24001 cgcactattg aagacctcaa cgccattgaa tcctgcgccc gtcgcagcaa gcgcggtgcc
    24061 gttgttggtg gcggcctgtt aggtctggaa gccgctggcg cgctgaaaaa cttaggcatt
    24121 gaaacccacg ttatcgaatt tgcccctatg ctgatggcgg aacagcttga tcagatgggt
    24181 ggcgagcagc tgcgtcgcaa aatcgaaagt atgggcgtgc gcgttcacac cagcaaaaac
    24241 acccttgaga ttgtgcagga aggtgtcgaa gcgcgtaaaa ccatgcgttt tgccgacggc
    24301 agcgaactgg aagtcgactt tatcgtcttc tctaccggta tccgtccgcg cgataagctg
    24361 gcaacccagt gtggtctgga cgttgctccg cgtgggggta tcgtcattaa tgattcctgc
    24421 cagacttccg atccggatat ctacgccatc ggtgaatgcg caagctggaa caaccgtgta
    24481 tttggtctgg tagcacctgg ctacaaaatg gcgcaggtcg ccgttgacca tattctcggt
    24541 agcgaaaacg cctttgaagg tgctgacctt agcgccaagc tgaaactgct gggcgtagac
    24601 gtaggcggta ttggtgatgc gcacggtcgc acgcctggcg cacgtagcta cgtttacctc
    24661 gacgaaagta aagagatcta caaacgcctg attgtcagcg aagacaacaa aaccctgctc
    24721 ggtgcggtac tggtgggcga taccagcgac tacggtaacc tgctgcaact ggtgctgaac
    24781 gctatcgagc tgccggaaaa cccggattct ctgattctgc cggcgcactc gggtagcggt
    24841 aagccgtcta tcggtgttga taaactgccg gacagcgcgc aaatctgctc ctgcttcgac
    24901 gtcaccaaag gtgatctgat tgctgccatc aacaaaggct gccacaccgt tgcggcgctg
    24961 aaagccgaaa ccaaagcggg tactggctgc ggtggctgta tcccgctggt cactcaggta
    25021 ctgaacgcgg aactggcgaa acaggggatc gaagttaaca acaacctgtg cgaacacttt
    25081 gcttattcgc gtcaggaact gttccatttg atccgcgttg aaggcattaa aaccttcgaa
    25141 gagctgctgg cgaaacacgg caaaggctac ggttgtgaag tttgtaaacc gaccgtcggt
    25201 tcgctgctgg cctcctgctg gaacgaatac attctgaagc cggaacatac tccgctgcag
    25261 gattctaacg acaacttcct cgctaacatc cagaaagacg gcacctactc ggtgatcccg
    25321 cgttctccgg gtggtgaaat caccccggaa gggctgatgg cggtaggtcg tatcgcgcgt
    25381 gaatttaatc tctacaccaa aatcaccggc tctcagcgcc tggcgatgtt tggcgcacag
    25441 aaagacgatc tgccggagat ctggcgtcag ctgattgaag ctggcttcga aaccggtcat
    25501 gcctatgcga aagcactgcg tatggcgaaa acctgcgtgg gtagcacctg gtgccgctac
    25561 ggcgttggcg acagcgtcgg cctcggcgtg gaactggaaa accgctacaa aggcatccgt
    25621 acgccgcaca aaatgaagtt cggtgtctcc ggctgtaccc gtgaatgttc agaagctcag
    25681 ggtaaagacg tgggtatcat cgccactgaa aaaggctgga acctgtatgt ttgcggtaac
    25741 ggcggcatga aaccgcgtca tgcggatctg ctggcggcgg atatcgatcg cgaaacgctg
    25801 attaaatatc tcgaccgctt catgatgttc tacatccgta ctgccgacaa actgacgcgt
    25861 accgcaccgt ggttagaaaa cctcgaaggc ggcatcgatt acctgaaagc ggtgatcatt
    25921 gacgacaaac tggggctgaa cgcacatctg gaagaagaga tggcgcgcct gcgtgaagcg
    25981 gtagtgtgtg agtggactga aacggtcaat acgccgtctg cgcagactcg cttcaaacac
    26041 ttcatcaaca gcgacaagcg tgacccgaac gtgcagatgg tgccagagcg cgaacagcac
    26101 cgtccggcaa cgccgtatga acgtatcccg gtaactctgg tggaggacaa cgcatgagcc
    26161 agtggaaaga catctgcaaa atcgatgaca tcctgcctga aaccggcgtc tgcgcgctgt
    26221 taggtgacga gcaggtagca attttccgcc cgtatcacag cgaccaggtg tttgcgatca
    26281 gcaacatcga cccgttcttc gagtccagcg tgctgtcacg cgggctgatt gcggaacacc
    26341 agggcgagct gtgggtcgcc agcccgctga aaaaacagcg ttttcgctta agtgatggct
    26401 tgtgcatgga agacgaacag ttttccgtca agcattacga agcacgagtg aaagacggcg
    26461 tggtacagct gcgcggttaa tgttttaacg ggaggcggaa tgcctcccct ttttgcatgg
    26521 tcctgtaata accttcggta tattgcagga cattttttaa actttttgtt ttattttttg
    26581 tttttatttt ttaaaggata atcaaatgtt tacagacact attaataagt gtgcggctaa
    26641 tgctgcgcgc attgaacgcc tgtcggcaaa taacccgctc ggcttttggg tcagctccgc
    26701 catggcgggc gcatatgtgg gtcttgggat catcctgatt ttcacgctcg gtaatttgct
    26761 cgacccgtcc gtacgtccgc tggtgatggg cgcgaccttt ggtatcgcct taacgctggt
    26821 gattatcgcc ggttctgaac tgttcaccgg gcacaccatg ttcctcacct ttggggtgaa
    26881 agcgggcacc atcagccacg ggcaaatgtg ggcaatcctg ccgcaaacct ggctgggtaa
    26941 cctggtcggt tccgtcttcg ttgccatgct ctatagctgg ggcggtggta gcctgctgcc
    27001 ggtagatacc agcatcgttc actccgtcgc gctggctaaa accactgcac cggcaatggt
    27061 actgttcttc aaaggtgcat tgtgtaactg gctggtttgt ctggcaatct ggatggcgct
    27121 gcgcactgaa ggggcggcga aatttatcgc aatctggtgg tgtctgctgg catttatcgc
    27181 gtccggctac gagcactcca tcgctaacat gacgctgttc gcgctctcct ggttcggcaa
    27241 ccacagcgaa gcctacacgc tggcgggtat tggtcataac ctgctgtggg tgacgctggg
    27301 taatacttta tcgggtgccg tattcatggg attgggttat tggtatgcta cgccgaaagc
    27361 gaatcgtccg gttgcggaca aatttaatca aactgaaacg gctgccggtt aattactaag
    27421 gggtttttac gtggatcatt tgcctatatt ttgccaatta cgcgatcgcg actgtctgat
    27481 tgtcggcggt ggtgatgtcg cggaacgcaa agcaaggttg ctgttagacg caggcgctcg
    27541 cttaacggtg aatgcattag cgtttattcc acagttcacc gcatgggcag atgcaggcat
    27601 gttaaccctc gtcgaagggc catttgatga aagccttctc gacacctgct ggctggcgat
    27661 tgcagcgacg gatgatgacg cgcttaacca gcgcgtcagc gaagccgctg aagctcgtcg
    27721 catcttctgt aacgtggtcg atgcgccgaa agccgccagc tttattatgc cgtcgattat
    27781 tgaccgctca ccgctcatgg tcgcggtctc ctctggcggc acctctccgg ttctggcacg
    27841 cctgttgcgc gaaaaactcg aatcactgct gccgttgcat ctgggccagg tggcgaaata
    27901 tgccgggcaa ttacgcgggc gagtgaaaca acagttcgca acaatgggtg aacgtcgccg
    27961 tttctgggaa aaattgttcg ttaacgaccg cctggcgcag tcgctggcca acaacgatca
    28021 gaaagccatt actgaaacga ccgaacagtt aatcaacgaa ccgctcgacc atcgcggtga
    28081 agtggtgctg gttggtgcag gtccgggcga tgccgggctg ctgacgctga aaggactgca
    28141 acaaattcag caggcagatg tggtggtcta cgaccgtctg gtttctgacg atattatgaa
    28201 tctggtacgc cgcgatgctg atcgcgtttt cgtcggcaaa cgcgcgggat accactgtgt
    28261 accccaggaa gagattaacc agatcctgct gcgggaagcg caaaaaggca aacgcgtggt
    28321 gcggctgaaa ggtggcgatc cgtttatttt tggccgtggt ggcgaagagc tggaaacact
    28381 gtgcaacgcg ggtattccgt tctcggtggt tccgggtatt accgcagctt ctggttgctc
    28441 tgcctattcg ggtattccgc tcacgcatcg cgattatgcc cagagcgtac gcttaattac
    28501 cggacactta aaaaccggtg gcgagctgga ctgggaaaac ctggcggcag aaaaacaaac
    28561 gctggtgttc tatatggggc tgaatcaggc cgcgaccatt cagcaaaagc tgattgaaca
    28621 cggtatgcct ggcgaaatgc cggtggcaat tgtcgaaaac ggaacggcag tcacgcagcg
    28681 cgtgattgac ggtacgctca cacagctggg cgaactggcg cagcaaatga acagtccatc
    28741 gctaattatt attggtcggg ttgttggcct gcgcgataaa ctgaactggt tctccaacca
    28801 ttaatttaac ccgggccaga gaattctggc cttcttaatg actcttttta tatattcagc
    28861 aaataaaaca taacccctta ataaatatta tctgctaatg ggttatattc accgttacag
    28921 cgacttacaa aattaagtca ttcgctcact gacgccaatt aaatattcct gtatcgttct
    28981 taatgagcag tctgcttata ttcatataat caatgaatat taattaatat ataatacata
    29041 gggaatgtaa atgaacaaat ttattaaagt tgcactggta ggtgcagtac tggctacgtt
    29101 aactgcatgt actgatcata ttgaaaaccg tgataagaac tgctcttacg actacctgct
    29161 gcacccggca atttctattt ctaaaatcat tggcggttgc ggtcctactg cacagtaagt
    29221 ctctgacaga aatcggctaa caccggttta attgcccgac gccctctgga ttttccgagg
    29281 gcgtatttgt ttctatatcc ttcttgctcc ccacccgtaa aaatattttc actttaattt
    29341 caattggata accacattca ttagccacag cgcgatcctg tgcgaaattt tgtgatcttc
    29401 ctccacatta cataacatca tatgttgtta tattcatcat gcattgtcat gttacctttt
    29461 aaatgactgc aaactctccc cctacaacgt gttggacaag aaaaaggtat cgctatggga
    29521 agccaggaac tccaacgcaa gctcggattt tgggccgttc ttgcaatcgc cgtcgggaca
    29581 accgtcggct ccggtatttt tgtatctgtg ggtgaagtgg caaaagcagc gggcacgccg
    29641 tggctgacgg tgctcgcgtt tgtcattggc gggttaattg tgatcccgca aatgtgcgtc
    29701 tatgcggaac tatccaccgc ttatccggaa aatggcgcag attatgttta tctgaaaaat
    29761 gccggaagcc gaccgctggc tttcctctcc ggctgggcca gcttctgggc caacgatgcg
    29821 ccgtcattgt cgattatggc gctggcgatt gtcagcaatc ttggcttttt aacgcctatc
    29881 gatccgttgc tcggtaaatt tatcgccgcc ggattaatta tcgcctttat gttgctgcac
    29941 ctgcgctccg ttgaaggcgg cgcagcgttt cagacgctaa ttaccatcgc caaaattatc
    30001 ccgttcacta tcgtcattgg tcttgggatc ttctggttta aagcggagaa ttttgcctcc
    30061 cctaccacca ctgcgattgg cgcaacgggc agctttatgg cgctgctggc ggggatctct
    30121 gccaccagtt ggtcgtatac cggcatggcc tctatctgtt atatgaccgg cgaaattaaa
    30181 aaccccggca aaaccatgcc acgagcgctg attggctcct gtctgctggt tctggtgctc
    30241 tacaccctgc tggcgttggt gatttccggc ctgatgccct tcgacaaact cgccaactct
    30301 gaaacgccga tttccgacgc cctgacctgg atcccggcac tcggcagcac cgccgggatc
    30361 tttgttgcca tcacggcgat gatcgtcatt cttggttcgc tttccagctg cgtgatgtac
    30421 cagccgcggc tggaatacgc gatggcgaaa gacaacctgt tcttcaaatg cttcggccat
    30481 gtgcatccga aatacaacac gccgaatgtc tccatcatcc tgcaaggggc gctggggatc
    30541 ttcttcatct tcgtttccga tctcaccagc ctgctgggtt atttcaccct ggtgatgtgt
    30601 ttcaaaaata ccctcacctt cggctccatc atctggtgtc gtaaacgcga cgattacaaa
    30661 ccgctgtggc gtactccggc tttcgggctg atgaccaccc tcgccattgc atcaagcctc
    30721 attctggtcg cctcaacctt tgtctgggca ccgattcccg gccttatctg cgccgtcatc
    30781 gttattgcca ctggtctgcc tgcttacgcc ttctgggcga agcgtagccg ccagctcaac
    30841 gctttgtcgt aatgttatct ggagaaaata aaatgttgga tattgataaa agcaccgtgg
    30901 actttctggt caccgaaaat atggttcagg aagtggaaaa agttctcagc catgacgttc
    30961 cgctggtgca cgccatcgtg gaagagatgg tgaagcgcga cattgatcgt atttatttcg
    31021 ttgcctgcgg ctcgccactc aacgcggcgc aaacggcgaa acatctggcg gatcgctttt
    31081 ccgatcttca ggtctacgcc atttccggct gggagttctg cgataacacc ccgtatcgcc
    31141 tcgacgatcg ttgcgcggtg attggcgttt ctgactacgg taaaacggaa gaggtaatca
    31201 aagcgctgga actgggtcgg gcctgcggcg cgcttactgc ggcattcacc aaacgcgcgg
    31261 acagcccgat tacctcggcg gcggaattta gcattgatta tcaggccgac tgtatctggg
    31321 aaattcacct gctgctctgc tacagcgtgg tgctggagat gatcacccgc ctcgcgccga
    31381 acgcggaaat cggcaagatc aaaaacgatc tcaagctgtt gccgaatgcg ctcggtcatc
    31441 tggtacgcac ctgggaagaa aaaggccgcc agcttggtga actggccagc cagtggccga
    31501 tgatttatac cgttgctgcg ggtccgctgc gtccgctggg ttacaaagaa ggcattgtaa
    31561 cgctgatgga atttacctgg acgcacggct gcgtgattga gagcggagag ttccgccatg
    31621 gcccgctgga gattgtcgaa ccggacgttc cgttcctgtt cctgctcggc aatgatgaaa
    31681 gtcgccacac caccgaacgc gccattaact ttgttaaaca gcgtactgac aacgtgatcg
    31741 tcatcgatta caccgaaatt tcgcaagggc tgcacccgtg gctggcaccg ttcctgatgt
    31801 tcgtgccgat ggagtggctc tgctactacc tgtctattta caaagatcac aacccggatg
    31861 aacgccgcta ttacggtggt ctggtggaat attaatccct ctcccggccc ggcaacgggc
    31921 cggatttacg caaggagtta cccgatgaaa acaggtatgt ttacctgcgg ccaccagcgg
    31981 ctgcctattg aacacgcatt ccgtgatgca agcgagctgg gttacgacgg catcgaaatt
    32041 tggggcggtc gcccgcacgc gttcgcgccg gacttaaaag cgggcggcat caaacaaatc
    32101 aaggcgctgg cgcagacgta tcagatgccg attatcggct atacgccaga aaccaacggc
    32161 tatccgtata acatgatgct gggcgatgaa catatgcgtc gcgaaagcct cgacatgatc
    32221 aagctggcga tggatatggc aaaagagatg aacgcgggtt atatgctgat ttccgcggcc
    32281 cacgcgggct atctcacgcc acctaatgtt atctggggac ggctggcaga gaacctaagc
    32341 gaactgtgtg agtacgcgga aaacatcggc atggatctga tcctcgaacc gttaacgccg
    32401 tatgaatcga acgtcgtgtg taatgctaat gatgtgcttc atgcgctggc gctggtgcct
    32461 tcgccgcgcc tgttcagcat ggtcgacatt tgcgcgccgt atgtccaggc ggaaccggtg
    32521 atgagttatt tcgacaaact gggcgataaa ttacgtcatc tgcatattgt cgacagcgac
    32581 ggggccagcg acacgcatta cattcctgga gaaggcaaaa tgccgctgcg ggaactgatg
    32641 cgcgatatta ttgatcgggg ctatgagggt tactgtacgg tggagctggt gacgatgtat
    32701 atgaacgagc ccagactcta tgcccgccag gcgctggaac gctttcgcgc gctgctgccg
    32761 gaggatgaga gatgaaaccc ctggcgacaa tcggcgataa ctgcgtcgat atctacccgc
    32821 aactgaataa agcgttttct ggcggtaatg cggtcaatgt ggcggtgtac tgcactcgct
    32881 acggcataca gccgggatgc attacctggg tgggtgacga tgactacggc acaaagctga
    32941 agcaggatct cgcccgcatg ggcgtcgata tcagccatgt ccatacgaaa cacggcgtta
    33001 ccgcacaaac tcaggtggaa ctgcacgaca atgatcgcgt ttttggcgac tacaccgaag
    33061 gcgtgatggc cgactttgcc ctgagtgaag aggattacgc ctggctggcg cagtatgaca
    33121 ttgtgcacgc ggcaatctgg ggacatgcgg aagacgcatt cccacagctg cacgctgcgg
    33181 gcaaacttac cgctttcgac ttctccgaca agtgggacag cccgctctgg cagacactgg
    33241 tgccgcatct cgattttgcc tttgcctccg caccgcaaga agacgaagcg ctgcgtctga
    33301 agatgaaagc gattgttgcc cgtggcgcag gaacagtgat tgtcacgctg ggtgaaaacg
    33361 gcagcattgc ctgggatggc gcgcagttct ggcgtcaggc tcctgaaccg gtgacggtta
    33421 tcgacaccat gggtgccgga gattcgttca ttgccggatt cctttgcggc tggtctgcgg
    33481 ggatgacatt accgcaggcg atggcgcagg gaacggcgtg cgcggcacaa actattcagt
    33541 accacggtgc ctggtaggca taacgttggc gtgagcatct tcacgccaac gtgctgttac
    33601 ttgccggaaa acgaccctat aatccgtgta attcattctt tatttcaggg tcgattatgt
    33661 cagctacgga ccgctactct catcaactcc tctacgctac cgtccgccag cggttgctgg
    33721 atgatatcgc gcagggggtt taccaggccg ggcaacagat ccctaccgaa aacgagcttt
    33781 gtacgcaata taacgtcagc cgcattacta ttcgcaaagc tatcagcgac ttagtggcag
    33841 acggcgtact gatccgctgg cagggcaaag gcacctttgt acaaagccag aaagtagaaa
    33901 acgccctgct tactgtcagt ggttttaccg attttggcgt ctcacaaggc aaggcgacga
    33961 aagagaaagt gatcgagcag gaacggatca gcgccgcgcc gttttgcgaa aagctgaaca
    34021 tccccggtaa cagcgaagtg ttccatctct gccgggtgat gtatctcgat aaagagccgc
    34081 tgtttattga tagttcgtgg atcccgctgt cgcgttatcc tgactttgat gagatttacg
    34141 tcgaaggaag ctccacctat cagttatttc aggagcgttt cgacacgcga gtggtcagcg
    34201 acaaaaagac catcgatatc tttgccgcca cccgcccgca ggcaaaatgg ctgaaatgcg
    34261 aactgggcga accgttgttt cgcatcagca aaatcgcctt tgatcagaat gataaaccgg
    34321 tgcacgtctc cgaactcttc tgccgcgcca atcgcatcac cttaacgatt gataataaaa
    34381 gacattaacc gtaggccgga taagatgcgc cagcatcgca tccggcgatg ctggcgcact
    34441 gaattttaca tcccgcacat tcccctcacc ctaaccctct ccccagaggg gcgaggggac
    34501 cgttcagtac ggaatttagc ttgacggtgc tatccagcat taaatactgg caatactctc
    34561 acgcaaaatc cgcagcaccg tctcttcacc gctgcgattc gggttaatgc gaatcgcgca
    34621 atgttcgagc tgtggattcg cctggcgaaa cgtcccggaa agtcgataaa aaagtggcgg
    34681 gatttcatat ttcgactctg caccgaccgg gtaaggcaac gcgccacgct tttgcgcttc
    34741 ttccagcacc ctggcggcaa tcggctgatg aaactcgaca atcaacacct tcgactgcgc
    34801 attggcaatg acggcgcttt tcacttccgc caccgcgccg ccgttaagca aagccagcaa
    34861 ccgttcggat actcccgcct gcaccgcatg catcactggc gcgaacacca gaccacgcaa
    34921 gacttccagc gcctgtgagc cctggatctg gctaccgccg gagtaaagcg tggcgcgaat
    34981 ccgactaata acatcggcat cgccaaccac tgcaccaacg ccctccggcc caaacagctt
    35041 gaagcaggag aatgtcgaga cattcgcgcc gcattcacag ccgattcgcg ctaccttcat
    35101 caccgcatag ttgtcatcgg ttaaggctgg aacacttgcc gcgcgcaacg ttgccagcac
    35161 atctgccagc acgtagctgt cctgcggctg ctggcgcgta tgctgcacca gcgccgcatc
    35221 cggttgttgc tcgtcgacga cctgcttcag tgccgacagg tcattgaaat caacagtaat
    35281 aagcgtcagc cccatctgct caataataac ccgtgtcgtc gggtaaacag gcgcgtcatg
    35341 caccagaaga cgctgccccg gtttgagcaa agccgccagc ccggcgcgaa tcgcgccagt
    35401 ccccgcgccc tgcaccagcg ccgcagcctg tgcgtgaaat gcatcagcca gcacctgctc
    35461 cacccgttgg gtaatacacg gttgattcag ccccggcgtt aagcccaaat caccgctggt
    35521 tagaaactcc gcgccgggaa agtgacggca aatcgtgtcc accagcgcaa actgcttttg
    35581 ctgcgcctca ataagcgtca ggctttgcag aggaaacgtc ttcataggtg ccttccttaa
    35641 gccggaacga acaggccaag ccagtaaaga acattcagca gaataccggt gatcattact
    35701 gccacgaccg gtgccgccat tttctgtacc ggacggccca gggattcgtt gaggaagtaa
    35761 atggcaaccg cgatagagaa tccggtataa cccgccatct taattgctgc gaaaatcgaa
    35821 ccgaccagca gcgccacttc catcagcata ttcatggcgt tacggatgtt atccgacgca
    35881 ttacgcaccg atgggtagcg tcccagccat ttgccgatcg aacgaagcaa taagacttcc
    35941 gccgaaataa ccactgcgcc taataccgct gcaaccatcg gattcggcga gagatagccc
    36001 accgcataaa caaaggtaaa gcccgcaact gcatatacgc cggtggctaa cgcggtggtg
    36061 gcaatcaacg gcacaaaacc cagtccgcgc ataaattccg ccagagcagc ctggttgatc
    36121 agcgtttgtg attgttccgg cgtcacgcct gcggaatatg ctttctccag agtgaagatc
    36181 gacacttcac taccggcgaa aatcttcatg ctggcaacgg cggcaatcaa tgctcccacg
    36241 atggcgatat acggtaagtt tttgataatc cgcgacgtac gttcttcgaa caccgaaagc
    36301 ccgctggcat caatgtcatt ttcatcacga tgacgcaggt cgtgagttat ggcgatcccc
    36361 agcagcatca ccatgccaat aaagatttcg atggattccg gattgagatg cgggaaatag
    36421 cgcacgacta ccacacgggt catcagtacc accacggcgg cgatcagact ttgcttccag
    36481 ccaaactggt agaaaattgc caccagcggg aacagtgcaa aagcagaaac caccggcgag
    36541 cttaattccc cgaggctacc taatacatcc accggcagcg cggtcagcag ctggtttact
    36601 ggcaacagac aagtaaggat caacacgccc cagatagcgc caagaccaaa cgccatcagg
    36661 ctgtttatcg ccagtacgcc gaggatatcg gtaggaagaa agagtaacca tgcgtttagc
    36721 agcccggttt tcagggtaaa agagatcccc accgaggcca caaaaccaat gctcagacca
    36781 aaagcgatac tccccgcctc gcgacggttc atatagcctt caatcagttg cggcaggatc
    36841 gggcggatgc cgtcatgaaa aacagccgcc gagcgatgcg ccagaagcga tgtcataccc
    36901 gtcaggcacg ccaccacgat aatctgaata tacagatcca tagtcgagcc ttttatttca
    36961 gatggttaat caacatcgga atcgcgtgtt cgacgtgctc aacggaaagg ccaaacgcca
    37021 cttttccttc ggcgatcatt ttggcgatat gctcgtcttt cgctttaatg cctggtttgg
    37081 caatggtgca acttttgtta tagccaatca ccgcgatggc aatcgacaat gccgcgcccg
    37141 caccggtatt acacgcgccg atgtaataat ccagttgccc ggatttcacc ttcattgccg
    37201 cttccatgtc gttgtgaatg aaaacttcaa aacagccagg agccgtcgct tcaatagttt
    37261 ttttaatctg ctcacgctgt aagcctgcaa cgccaatctt tttcatgagt ctgtccttat
    37321 tggaaaaatt gagagggatt ttcacgtaac atcacatcca catcggcctg actgaatcct
    37381 gactggcgca attgcggaat aaaggtggtc aataaatagt catagccata accaccattg
    37441 gcttttaaat gggagcggcg cgtaatatcc atcgacagca tgacgcggtt cagcaaccca
    37501 cggtcacgta gcgcgtggag catcgcaatg cgcttttcgt ccgggtagta actgttctta
    37561 ccaatggtgt cgaactgcac gtacgcgccg agatcgatca tcttcaaaat gttgtcgagg
    37621 ttgtctttca gatcgcagtg accaacggtg acgcgcgaaa gatcaactcc gtgggcttgc
    37681 aacagagcca gttgttccag ccccatggtg ctgaacgacg tatgcgtgga gatcgggcgt
    37741 ccggtctggt tatgcgccag cgcagcggca ataaatacct tctcttccag cggcgtaatc
    37801 tttccttcgc tggagccgat ctccgcgatg atcccggctt tcagctccgt gccatcgata
    37861 ccctgttcaa tttcatcgac catctcctgc gccagttcct gcacgctgcg ggtcaccaca
    37921 tgttccggga aaaacgcatc ctagtaataa ccagtacagg ccaccacgtt gatcccggtt
    37981 tcgcgcatta catcgagcat aaattgcgca ttgcgcccca tgtaacggtt ggtcatctca
    38041 atcacattac gtacaccccg ggtcatcagg tcgttcatct cctggcaaat gaacgcatac
    38101 tgatcaaggc ggcagtccac gttgttttta aagccggaga gatcaatatg cagatgctca
    38161 tgggccaggg tgtaacccgt cggatcaaaa ctcatagggt gtctcctgca aaccggaggg
    38221 aggaaagaaa agagcgacca ttttgtggcg gtggcgcacg gaaaaattca cacacggtag
    38281 ctcccacatc agaaagcgtg gtgcgcacac cgagctgcgt ggcgaccagc ccttgctgat
    38341 aaaccagcac tggcaccact tcacgggtat ggtggctgtg accaatggtc ggatcgttac
    38401 cgtgatccgc catcacgacc aggcaatcat ctggctgcat cgcctcaaca agccgggcaa
    38461 ggttacggtc aacgacctgc aaacgttcgg cataacgtgc gacgtcttct gcatgaccag
    38521 cgaggtcggt ttcctggata ttggtgcaaa taaacgccgt cggatgcgtg ttaaattcgt
    38581 cgagggtgat atccataatc cgctggctat ccaccaggtt ttgccagctc acgccataag
    38641 gattgctgac gatatctgcc accttaccca ccagcacggt tggcacgccc gcctcataca
    38701 gtttttgcgg cacctgcact ttttcatcaa caccgtagcc catatgcacg acctggaaac
    38761 cgttgtcata agcgccagaa cgcggcgcat tgatgccaat aaagcgccct tctttgcttt
    38821 ctgcggcatc gagaatacgc tgactgtcgg ttaacaagcc gccaaatgta atgacccgac
    38881 cgacctgtac ctgctcacgc acgatacgac caattttgat tgcgtcgtca aaagagatca
    38941 cagagagatt ggcggtaatg ttatagacct ggcctaaatc cgcctcgaga ttatcgccaa
    39001 tcgcaaccgc ctgattgacc cacagaaatt gcagatcatc gccacggcgc tccacctgcc
    39061 agccagcgga aactaatgcc tgctcaacat ggtcaatcac atcgcgaaaa ggcatccgca
    39121 gcggcggtaa cgggcgcgtg cctaaaattt cctgatgccc cataaaggta tcgccacctt
    39181 catgttgcag ctctgccacg ccccaggttg cggaatctga cggctgcata tcgcctggcg
    39241 cataacccaa tgcgttgatt agccccagct tctccagcgt tggtagctgc aaatgcggca
    39301 actggctcag gatgtgacca caggtattcg ctcccgcatc ttgcggacgc accagcgtga
    39361 catctttcat tgcccctacg ccaaagctat caatcactaa caccacaaat cgcgccatta
    39421 cgcccccaga gagtttccaa gactgtcata acaaccaacg attttcggtt cgccacggtg
    39481 aataccggac accagcacca catcgctacg ggtgacaaaa atctgcgtgc ggaaacagag
    39541 caccactgca ctgcttaccg gaaactcgcc tgccagcgac agggtgtagt cgatactgct
    39601 gtcatccaca gttttgagat tggtttcagt aatcttttga ttttctggcg taaacaccaa
    39661 cgcatgttgc gcatgaccac gacggtagta accgccgccg tagcagtagc tgtcgccacg
    39721 gaaatgatgg gagatttcac tcaaccagag catcgcgata cgttcagact gatcgccctg
    39781 ctggtttgcc ggaatagtgc ccgtcagcgc atgaccgggt tcggcatgag tcacaccgta
    39841 ttgcgccagt aatggcagcg aagtgcagct ggtcgctgaa ggcgcgttca gttgctcaat
    39901 cgcaatacca gatttcgcca gttgatcccg tgcttgtacc agcgtgtgaa gattcggtgt
    39961 cggcaaaact ttcccggcag cctcatccca aagcaggcaa gggaaatggg taagtccggc
    40021 taaatgcagc cccggcagtt tcttaatttc agcaaccacc tcgtgcagcg aatgctgtac
    40081 aaaaccgctc tcctgccccg gataaagaaa atcatcgtcg ctataaactt tgagcagcac
    40141 agactggatt cgcccggcct tcaccgccgc cgcagaaact tcccgcgctt tgtcgagagt
    40201 aaacacggtg atgacgtcgg tgccctgttc aacggcgtca gcaacctgat gacaagggat
    40261 ttgtaccaga tgcccctgat gcgccaccgg caaatcagcg cggcgcatga ctcgcgcctc
    40321 tttgtaatcc accgccacaa tgccgctgta gcccaacgcc agcagttttt ccgccagcca
    40381 tgggttacga ccgaattgtt tggtcatcag atacagttca atgccgtaaa gccgcgccgt
    40441 ctcaatcagc cgcttaccgt tttccagtat ctgatccacg tcgatcaccc agctgtccgg
    40501 ggcgattttg ccctgctgcc acaaacttag tgcggcggaa atcagcgccg ggttctggcg
    40561 tttcaatgct tctacaaaca tcttgttgcc tcgtttctgt tccttgaatt aaatattcat
    40621 ttttttgaat atttaggttg tgcgacgtgg atttgtcggg agcttcaaac ctcttcgtta
    40681 gcagccatcc ataatccata aaggttggcc agcaaatagc cttcttcaca cgggttaact
    40741 tccagtgcga attccttcaa caacacctga tgcaattgca caacggcttg ccagtggctg
    40801 gattgcgcca gttctgccag caactcgtta tccagcggct ctatttcttc accacggcga
    40861 ctgcgcatca gtgcactcgc catatgtgtc atcgccatcg ttccttgctc actgcgcacc
    40921 ggcagatggc actctgtttc cagtacgttg acgacctgca tcatgccttt gcagatatcc
    40981 ttatcaataa cgcctgcctc gcaaagcagg ttgagtctgg tttccatatc agatgccttt
    41041 cgtttaataa ctcggttcct gttccccact cactacccgt tgttgatggg cgagcagggc
    41101 gtgtttatct acaacggcac gcagcagttg ttgcattggg taatcatcga ctcgcgtcag
    41161 gaccacggct tcggtggcct gtaaaaatcg cggatcgtct gtgagcggcg tcgcctctaa
    41221 tcccagcatg gtcagttcgt tttccgccac cacgttccag atcaccgcat cgacatcgcc
    41281 tttaacaatg cgttgtaaac tctcgtgata agagagatcg acacgttcca catcactatc
    41341 gccaaaaaag acatcggtca tgattttctg atccgccgaa cggttatcca gccccacgcg
    41401 cttcacgttt gcggactcgc ctttacggca aatcaactgg tgctcgccaa cgtaggtgtg
    41461 cggccccaac tccagcgcga ggcataaccc tttttgcgtg agataacttt ccgccgccag
    41521 tcgggaaacc accgccatgt catacacgcc gttaagtaaa cactccacgc gaatatccgc
    41581 gccgcgcata tgcgcatagt aaaaaggaat gccatcaaac tgagctttca gcccgctcgc
    41641 taaaccttcg tacaagcggg tatagggcaa aggcatcgca cataccacgt tgttgatatc
    41701 cacatgactc agcaatgctt tgttatccat ctcgaccaga taactgccat tgcgcccacg
    41761 gcgttcaatc cgtatcgctc cgcttgattc cagcgttttc aacgcggcct gcgtcaggcc
    41821 aaccgatgaa cggcattcag ttgccagctc atctatggtt ttcaacctat taccgcattt
    41881 ttcacccaac aaataacggg ccagcgtcgt gataacgacg ccttcttttt tgata
//
LOCUS       DAZGSP010000039        41894 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZGSP010000039 DAZGSP010000000
VERSION     DAZGSP010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.039"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            261..1319
                     /gene="citC"
                     /locus_tag="ACHK74_002208"
     CDS             261..1319
                     /gene="citC"
                     /locus_tag="ACHK74_002208"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFP0221025.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1334..1630
                     /gene="citD"
                     /locus_tag="ACHK74_002209"
     CDS             1334..1630
                     /gene="citD"
                     /locus_tag="ACHK74_002209"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFP0221026.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1627..2535
                     /gene="citE"
                     /locus_tag="ACHK74_002210"
     CDS             1627..2535
                     /gene="citE"
                     /locus_tag="ACHK74_002210"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFP0221027.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2546..4078
                     /gene="citF"
                     /locus_tag="ACHK74_002211"
     CDS             2546..4078
                     /gene="citF"
                     /locus_tag="ACHK74_002211"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFP0221028.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4082..4633
                     /gene="citX"
                     /locus_tag="ACHK74_002212"
     CDS             4082..4633
                     /gene="citX"
                     /locus_tag="ACHK74_002212"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFP0221029.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4608..5486
                     /gene="citG"
                     /locus_tag="ACHK74_002213"
     CDS             4608..5486
                     /gene="citG"
                     /locus_tag="ACHK74_002213"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFP0221030.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5537..7000
                     /gene="citT"
                     /locus_tag="ACHK74_002214"
     CDS             5537..7000
                     /gene="citT"
                     /locus_tag="ACHK74_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFP0221031.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7114..7920
                     /gene="rna"
                     /locus_tag="ACHK74_002215"
     CDS             7114..7920
                     /gene="rna"
                     /locus_tag="ACHK74_002215"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFP0221032.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8150..8560
                     /gene="rnk"
                     /locus_tag="ACHK74_002216"
     CDS             8150..8560
                     /gene="rnk"
                     /locus_tag="ACHK74_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFP0221033.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAHAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8698..8787
                     /gene="yldA"
                     /locus_tag="ACHK74_002217"
     CDS             8698..8787
                     /gene="yldA"
                     /locus_tag="ACHK74_002217"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFP0221034.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8791..10029)
                     /gene="ybdR"
                     /locus_tag="ACHK74_002218"
     CDS             complement(8791..10029)
                     /gene="ybdR"
                     /locus_tag="ACHK74_002218"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFP0221035.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10250..10678
                     /gene="uspG"
                     /locus_tag="ACHK74_002219"
     CDS             10250..10678
                     /gene="uspG"
                     /locus_tag="ACHK74_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFP0221036.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10799..12364)
                     /gene="ahpF"
                     /locus_tag="ACHK74_002220"
     CDS             complement(10799..12364)
                     /gene="ahpF"
                     /locus_tag="ACHK74_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFP0221037.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12535..13098)
                     /gene="ahpC"
                     /locus_tag="ACHK74_002221"
     CDS             complement(12535..13098)
                     /gene="ahpC"
                     /locus_tag="ACHK74_002221"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFP0221038.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13470..14216
                     /gene="dsbG"
                     /locus_tag="ACHK74_002222"
     CDS             13470..14216
                     /gene="dsbG"
                     /locus_tag="ACHK74_002222"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFP0221039.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14425..15327
                     /gene="ybdO"
                     /locus_tag="ACHK74_002223"
     CDS             14425..15327
                     /gene="ybdO"
                     /locus_tag="ACHK74_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0221040.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEHLLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15474..16694
                     /gene="ybdN"
                     /locus_tag="ACHK74_002224"
     CDS             15474..16694
                     /gene="ybdN"
                     /locus_tag="ACHK74_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP0221041.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKSKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            16667..17296
                     /gene="ybdM"
                     /locus_tag="ACHK74_002225"
     CDS             16667..17296
                     /gene="ybdM"
                     /locus_tag="ACHK74_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFP0221042.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(17297..18457)
                     /gene="ybdL"
                     /locus_tag="ACHK74_002226"
     CDS             complement(17297..18457)
                     /gene="ybdL"
                     /locus_tag="ACHK74_002226"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFP0221043.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATIWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            18566..19654
                     /gene="hcxA"
                     /locus_tag="ACHK74_002227"
     CDS             18566..19654
                     /gene="hcxA"
                     /locus_tag="ACHK74_002227"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFP0221044.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(19664..19861)
                     /gene="ybdD"
                     /locus_tag="ACHK74_002228"
     CDS             complement(19664..19861)
                     /gene="ybdD"
                     /locus_tag="ACHK74_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP0221045.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(20043..22148)
                     /gene="cstA"
                     /locus_tag="ACHK74_002229"
     CDS             complement(20043..22148)
                     /gene="cstA"
                     /locus_tag="ACHK74_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFP0221046.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(22329..22742)
                     /gene="entH"
                     /locus_tag="ACHK74_002230"
     CDS             complement(22329..22742)
                     /gene="entH"
                     /locus_tag="ACHK74_002230"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFP0221047.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(22745..23491)
                     /gene="entA"
                     /locus_tag="ACHK74_002231"
     CDS             complement(22745..23491)
                     /gene="entA"
                     /locus_tag="ACHK74_002231"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFP0221048.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDALVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(23491..24348)
                     /gene="entB"
                     /locus_tag="ACHK74_002232"
     CDS             complement(23491..24348)
                     /gene="entB"
                     /locus_tag="ACHK74_002232"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFP0221049.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(24362..25972)
                     /gene="entE"
                     /locus_tag="ACHK74_002233"
     CDS             complement(24362..25972)
                     /gene="entE"
                     /locus_tag="ACHK74_002233"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFP0221050.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(25982..27157)
                     /gene="entC"
                     /locus_tag="ACHK74_002234"
     CDS             complement(25982..27157)
                     /gene="entC"
                     /locus_tag="ACHK74_002234"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFP0221051.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            27532..28488
                     /gene="fepB"
                     /locus_tag="ACHK74_002235"
     CDS             27532..28488
                     /gene="fepB"
                     /locus_tag="ACHK74_002235"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP0221052.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(28492..29742)
                     /gene="entS"
                     /locus_tag="ACHK74_002236"
     CDS             complement(28492..29742)
                     /gene="entS"
                     /locus_tag="ACHK74_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFP0221053.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAKIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDG"
     gene            29853..30857
                     /gene="fepD"
                     /locus_tag="ACHK74_002237"
     CDS             29853..30857
                     /gene="fepD"
                     /locus_tag="ACHK74_002237"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFP0221054.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            30854..31846
                     /gene="fepG"
                     /locus_tag="ACHK74_002238"
     CDS             30854..31846
                     /gene="fepG"
                     /locus_tag="ACHK74_002238"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFP0221055.1"
                     /translation="MIYVSRRLLITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALSAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            31843..32658
                     /gene="fepC"
                     /locus_tag="ACHK74_002239"
     CDS             31843..32658
                     /gene="fepC"
                     /locus_tag="ACHK74_002239"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP0221056.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(32655..33788)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHK74_002240"
     CDS             complement(32655..33788)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHK74_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFP0221057.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(34004..37885)
                     /gene="entF"
                     /locus_tag="ACHK74_002241"
     CDS             complement(34004..37885)
                     /gene="entF"
                     /locus_tag="ACHK74_002241"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFP0221058.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINLAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSEKIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSARRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQIATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(37882..38100)
                     /gene="ybdZ"
                     /locus_tag="ACHK74_002242"
     CDS             complement(37882..38100)
                     /gene="ybdZ"
                     /locus_tag="ACHK74_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFP0221059.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(38103..39305)
                     /gene="fes"
                     /locus_tag="ACHK74_002243"
     CDS             complement(38103..39305)
                     /gene="fes"
                     /locus_tag="ACHK74_002243"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFP0221060.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWKTQLNANWRGSYCFIPTERDDIF
                     SVPSPDRLELREGWRKLLPQAIADPLNLQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKKSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            39548..41788
                     /gene="fepA"
                     /locus_tag="ACHK74_002244"
                     /pseudo
     CDS             39548..41788
                     /gene="fepA"
                     /locus_tag="ACHK74_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
BASE COUNT        10034 a        11459 c        10693 g         9708 t
ORIGIN      
        1 ttaaacctgc tggtcatttc gtaattacat taattaaaaa acttaaaacc attaaataaa
       61 taaaaaacca ctaactctat gtgaaataaa tcaaaatttc acgccgaaac actccttagg
      121 atgtatagcg aaaggagaaa aagatatacc tcgatcaccc ccctttctcc caagtgaaaa
      181 taaaaggtta tcagtttgca acattgaaca acattcgttg caaatcgata acaacatgca
      241 ccttcaggat actatttatt atgttcggca atgatatttt cacccgcgta aaacgttcag
      301 aaaataaaaa aatggcggaa atcgcccaat tcctgcatga aaatgatttg agcgttgaca
      361 ccacagtcga agtatttatt accgtaaccc gcgatgaaaa gcttatcgcg tgcggtggaa
      421 ttgccggaaa tattattaaa tgcgttgcta tcagtgaatc cgttcgcggt gaaggactgg
      481 cgctgacatt agccactgaa ctgataaacc tcgcctatga gcggcacagc acgcatctgt
      541 ttatttatac caaaaccgaa tacgaggcgc tgttccgcca gtgcggtttt tccacgctga
      601 ccagcgtacc cggcgtgatg gtgctgatgg aaaacagcgc cacgcggctg aaacgctatg
      661 ccgaatcgct gaaaaaattt cgtcatccag ggaacaagat tggctgcatt gtgatgaacg
      721 ccaatccctt cacgaatggt caccgttatc tgattcaaca agctgcggca cagtgcgact
      781 ggttgcatct gtttttagtc aaagaagatt cttcacgctt cccctatgaa gaccggttgg
      841 atctggtgtt aaaaggcacc gccgatattc cacgcctgac tgtgcatcgc ggctccgaat
      901 acatcatctc ccgcgctacg ttcccttgct acttcattaa agaacagagc gtcattaacc
      961 attgttacac cgaaattgat ctgaagattt tccgtcagta cctcgctccc gcgctgggtg
     1021 taactcaccg ctttgtcggt actgaaccct tttgtcgcgt taccgcccag tacaaccagg
     1081 atatgcgcta ctggctggaa acgccgacta tctccgcacc gcccatcgaa ctggttgaaa
     1141 ttgagcggct gcgttaccag gagatgccga tatccgcttc ccgggtacgt caactgctgg
     1201 cgaaaaacga tctcacggct atcgcgccgc tggtccctgc agtcacgctg cattatttgc
     1261 agaacctgct tgagcactcc cgccaggacg cggcagctcg tcaaaagacc cccgcatgag
     1321 aaacaggtga aaaatgaaaa taaaccagcc cgccgttgca ggcacccttg agtctgggga
     1381 tgtgatgata cgcatcgccc cactcgatac gcaggatatc gacctgcaaa tcaatagcag
     1441 cgttgagaaa cagtttggcg atgcaattcg caccaccatt ctggacgttc tcgcccgcta
     1501 caacgtgcgc ggcgtacagc tgaatgtcga tgacaaaggc gcactggact gcattttacg
     1561 tgcacgactg gaagccctgc tggcacgcgc cagcggtatc ccggctctgc catgggagga
     1621 ttgccaatga tttccgcttc gctgcaacaa cgtaaaactc gcacccgccg cagcatgttg
     1681 tttgtgcctg gtgccaatgc cgcgatggtc agcaactcct tcatctaccc ggctgatgcc
     1741 ctgatgtttg acctcgaaga ctccgtggca ttacgtgaaa aagacaccgc ccgccgcatg
     1801 gtttatcacg cgctgcaaca tccgctgtat cgcgatattg aaaccattgt gcgtgtcaac
     1861 gcgctggatt ccgaatgggg tgttaacgac ctggaagccg tcgttcgcgg tggtgcggac
     1921 gttgtgcgtc tgccgaaaac cgataccgct caggatgttc tggatattga aaaagagatc
     1981 ctgcgtatcg aaaaagcctg tggtcgtgaa cccggcagca ccggcctgct ggcggcgatt
     2041 gaatctccgc tgggcattac ccgcgcagtg gaaatcgctc acgcttccga gcgtttgatc
     2101 ggtatcgccc tcggtgcaga agactatgtg cgcaacctgc gtacagaacg ctccccggaa
     2161 ggaactgaac tgctgttcgc acgctgttcc attttgcagg ccgcgcgctc tgcgggtatt
     2221 caggcgttcg ataccgtcta ttccgacgct aacaacgaag ccggattcct gcaagaagcc
     2281 gcccacatca aacagctggg ctttgacggc aaatcgctga tcaacccgcg tcagattgat
     2341 ctgttgcaca acctctacgc accaacccag aaagaagtgg atcacgcccg ccgcgtcgta
     2401 gaagccgctg aagccgccgc tcgcgaaggc ctcggcgtgg tttccctgaa cggcaagatg
     2461 gtggacggtc cggttatcga tcgcgcccgt ctggtgctct ctcgtgcaga actttccggc
     2521 atccgcgaag aataaggcaa tcaaaatgac gcagaaaatt gaacaatctc aacgacaaga
     2581 acgggtagcg gcctggaatc gtcgcgctga atgcgatctt gccgctttcc agaactcgcc
     2641 aaagcaaacc taccaggctg aaaaagcgcg cgatcgcaaa ctgtgcgcca acctggaaga
     2701 agcgattcgt cgctctggtt tacaggacgg catgacggtt tccttccatc acgctttccg
     2761 tggcggtgac ctgaccgtca atatggtgat ggacgtcatc gcgaagatgg gctttaaaaa
     2821 cctgaccctg gcgtccagct ccctgagtga ttgccatgcg ccgttggtag aacacattcg
     2881 ccagggcgtg gttacccgca tttatacctc cggcctgcgc ggtccactgg cggaagaaat
     2941 ctcccgcggt ctgctggcgg aaccggtgca gatccactct cacggcggtc gtgtgcatct
     3001 ggtacagagc ggcgaactga atatcgacgt ggctttcctc ggcgtcccgt cctgtgatga
     3061 attcggtaat gccaacggct acaccggtaa agcctgctgc ggctccctcg gctatgcaat
     3121 ggttgatgcc gacaacgcaa aacaggttgt gatgcttacc gaagaactgc tgccttatcc
     3181 gcataatccg gcaagcattg agcaagatca ggttgattta atcgtcaaag ttgatcgcgt
     3241 tggcgatgct gcaaaaatcg gcgctggtgc tacccgtatg accactaacc cgcgcgaact
     3301 gcttattgcc cgtagcgctg cggatgtgat tgtcaactca ggctacttca aagaaggttt
     3361 ctccatgcaa accggcaccg gcggcgcatc gctggcggta acccgtttcc tggaagacaa
     3421 aatgcgtagc cgcgatattc gcgccgactt cgccctcggt ggtattaccg cgacgatggt
     3481 tgatctgcac gaaaaaggtc tgatccgcaa actgctggat gtacagagct ttgacagcca
     3541 tgctgcgcaa tcgctggccc gtaaccccaa tcacatcgaa atcagcgcca accagtacgc
     3601 taactggggt tcgaaaggcg catcggttga tcgtctcgac gtggtggtac tgagcgcgct
     3661 ggaaattgac acccagttca acgttaacgt gctgaccggc tctgacggcg tactgcgtgg
     3721 tgcttccggt ggtcactgcg ataccgcgat tgcctctgcg ctttccatca tcgtcgcgcc
     3781 gctggtacgc ggtcgtattc cgactctggt ggataacgtg ctgacctgca tcaccccggg
     3841 ctccagtgtc gatattctgg tcacagacca cggtatcgca gttaacccgg cacgtccgga
     3901 actggcagaa cgtctgcagg aagcgggcat taaagtggtt tccattgagt ggctgcgcga
     3961 acgtgcgcgt ctgctgaccg gtgaaccaca gccgattgaa ttcaccgacc gcgtcgttgc
     4021 cgttgtgcgt taccgcgatg gctcggtgat cgatgttgtg catcaggtga aggaataagc
     4081 catgcacctg cttcctgaac tcgccagcca ccatgcggtg tcaattcccg agctgctcgt
     4141 cagccgggat gaaaggcaag cacggcaaca cgtctggctc aagcgccatc ctgttccact
     4201 ggtctccttt accgtggttg cgcctgggcc gattaaagac agcgaggtca cacgccgaat
     4261 ttttaatcat ggcgtgacag ccttgcgtgc cttagccgca aaacagggct ggcaaattca
     4321 ggagcaggct gcactggttt ccgccagcgg gccggagggc atgttgagca ttgccgcccc
     4381 ggctcgcgac ctcaagctcg ccaccattga gcttgaacat agtcatcctc tcgggcggtt
     4441 atgggatatc gatgtcctga cgcccgaagg cgaaattctc tcccgccgcg actattcact
     4501 gccgcctcgc cgctgcctgt tgtgcgagca aagcgcagcc gtctgcgcgc gtggaaaaac
     4561 ccatcaactg accgatttac tcaaccgcat ggaggcactg ctgaacgatg tcgatgcctg
     4621 caacgtcaac taaaaccaca aagcttgcga cgtcattaat cgatgagtac gccctgctgg
     4681 gctggcgcgc catgctgact gaagtcaatc tgtcaccgaa accaggcctc gtggatcgca
     4741 ttaactgcgg tgcgcacaaa gatatggcgc tggaagattt ccaccgcagc gcgctggcga
     4801 ttcagggctg gttaccccgt ttcattgaat ttggtgcctg tagtgcagaa atggcaccgg
     4861 aagcggtact ccacggatta cgcccaattg gtatggcttg cgaaggtgat atgttccgcg
     4921 ccactgcggg cgtaaacacg cataaaggca gcattttttc tttagggctg ctatgtgcgg
     4981 caattggccg tttgcttcag ctcaaccagc cggtaacgcc aacaaccgtt tgttctacgg
     5041 cggcaagttt ctgccgtggt ctgaccgatc gcgaactgcg taccaataat tcacaactga
     5101 cggcaggtca acggttgtac cagcagcttg gccttaccgg cgcacgcggt gaagccgaag
     5161 cgggttatcc actggtgatc aaccacgcct tgccgcatta cctcactctg ctggatcagg
     5221 ggttagatcc tgaactggca ttgctcgata ccttgctcct actgatggcg atcaacggcg
     5281 ataccaacgt tgcttcgcgc ggtggcgagg ggggcctgcg ctggctacag cgcgaggcgc
     5341 aaacattatt gcaaaaaggg ggcattcgaa cccccgccga tctcgattat ctccggcagt
     5401 tcgacaggga gtgtatcgaa cgaaatctca gtccaggcgg cagtgccgac ctgctgatcc
     5461 ttacctggtt tttagcacag atttaattat ttaagcactt gataaatttg aaaatattaa
     5521 ttttcggaga acccgtatgt ctttagcaaa agataatata tggaaactat tggccccact
     5581 ggtggtgatg ggtgtcatgt ttcttatccc tgtccccgac ggtatgccac cacaggcatg
     5641 gcattacttc gctgtgtttg tggcaatgat tgtcggcatg atcctcgagc caattccggc
     5701 aacagcgatc agttttattg cggttactat ttgcgttatt ggcagtaatt acctgctctt
     5761 tgatgccaaa gaattagctg acccagcgtt taatgcgcaa aaacaggcgc tgaaatgggg
     5821 tctggctggt ttttccagca ccacggtatg gctggtattt ggcgcattta tttttgcatt
     5881 agggtatgaa gtttccgggt taggtcgtcg cattgccctt ttcctggtga aattcatggg
     5941 caaacgcacg ctgacgttgg gttatgcgat tgtcattatc gacattctgc tggcaccgtt
     6001 tacaccgtcc aacaccgcgc gtaccggggg tacggttttt ccggtcatta aaaacctgcc
     6061 gccgttgttt aaatcattcc ccaacgatcc gtccgcgcgt cgtattggcg gctatttgat
     6121 gtggatgatg gtcattagta ccagtctgag ttcgtccatg tttgtcaccg gtgcggcacc
     6181 aaacgtgctg ggtctggagt tcgtcagcaa aattgccggt atccagatta gctggttgca
     6241 gtggttcctc tgcttcctgc cggttggggt tatcttgctt atcattgcgc cgtggctttc
     6301 ctacgtgctg tacaaaccgg aaatcacaca cagtgaagaa gtggcaacct gggcgggtga
     6361 tgaactgaaa accatgggtg cgctgacacg cagagagtgg acactgattg gccttgtatt
     6421 gctcagctta ggtttgtggg tttttggcag tgaagtcatt aatgctactg cagttggtct
     6481 gctggcagtt tcgctaatgc tggccctgca cgttgtgcct tggaaagata ttacccgcta
     6541 taacagcgca tggaacacgc tggtcaacct ggcaactctg gttgtgatgg ctaacggcct
     6601 gactcgttct ggctttattg actggttcgc cggtaccatg agtacgcacc tggaaggatt
     6661 ctcaccaaac gcaacggtga ttgtactggt tctggtgttc tactttgcac actacctgtt
     6721 tgccagcctg tctgcgcaca ccgcaaccat gctgccggtt attctggccg tcggtaaagg
     6781 tattccgggc gtaccaatgg aacaactgtg tatcctgctg gtgctgtcta tcggtatcat
     6841 gggctgtctg acgccgtatg caaccggtcc tggggtgatt atttacggct gtggctatgt
     6901 gaaatcaaaa gattactggc gtcttggcgc aatcttcggg gtgatttaca tctctatgtt
     6961 gctgttggtt ggctggccga ttctcgccat gtggaactaa tacgttcgcc aaacgctgaa
     7021 taaatcatca cgcccgccag ataagcccac ctggcgggct tttttatgat ttaatagata
     7081 gtattcagtg tatgtatgag ttccacaccc attatgaaag cattctggcg taacgccgcg
     7141 ttgctcgcgg tttctctgct tcccttctct tctgccaacg ccttagcgtt gcaggcaaaa
     7201 cagtatggcg attttgatcg ctatgtcctg gccctctcct ggcaaaccgg attttgccag
     7261 agtcaacacg atcgaaatcg taacgaacga gatgaatgtc gcctgcaaac cgaaacgacc
     7321 aacaaagctg attttctgac cgtacatggt ctgtggccag gattgcctaa atcggttgct
     7381 gcccgtggtg ttgatgaacg ccgctggatg cgcttcggtt gcgctactcg cccaatcccg
     7441 aatctaccag aagcgcacgc cagccggatg tgttcatcgc cggaaaccgg attatcactg
     7501 gaaacggccg ctaaactaag tgaagtcatg ccaggagctg gcggacgttc ctgcctggaa
     7561 cgctacgaat atgccaaaca cggtgcctgc tttggttttg atccggacgc atacttcggt
     7621 acgatggtac gcctgaatca agaaattaaa gagagcgaag ccggaaaatt ccttgcggat
     7681 aattacggta aaacagtgag ccgccgtgac tttgacgccg cctttgccaa aagctgggga
     7741 aaagagaacg tgaaagcagt taagctaacg tgccagggta accctgcgta tttgactgaa
     7801 attcagatct cgatcaaagc tgacgccatc aacgctccgc tttctgcaaa ctcattcttg
     7861 ccacaaccac acccaggcaa ctgtggcaaa acctttgtga ttgataaagt gggttattaa
     7921 tcatctgatt tacaaaacac gctaattcgt cttccataaa ccgtgacgat taagaattat
     7981 acataatgcg ctgcattgag gaattccctg aatgtgacgc aaatcacttc aaagagtgag
     8041 caacagtcag tatcatcatg tgagttaaac cctcgccgcc tgacggtgag ggttttcttt
     8101 tgggtatgtt tatctgcgac actcgcagta ccgacgacat ggagtaaaaa tgtccagacc
     8161 aactatcatc attaacgacc tggatgccga acgcatcgat attctgctgg agcaacccgc
     8221 ccatgctggt ttgccaatcg ccgacgcgtt aaacgcagag ttggatcgcg cccaaatgtg
     8281 ttcgccagaa gagatgccac acgacgtggt gacaatgaac agccgggtta aattccgcaa
     8341 tcttagcgat ggcgaagtgc gtgtgcgcac gctggtgtat ccggcaaaaa tgaccgatag
     8401 caatactcag ctttccgtta tggctccggt aggtgccgca ctgctggggc tgcgcgttgg
     8461 cgattccatt cactgggaac ttccgggcgg cgttgcaacc caccttgaag tgctggaact
     8521 cgaataccag ccagaagctg ctggcgacta cctgctttaa tcccatctgc acaagcgata
     8581 actcgctttg cccagaggat gcactcgcat cctctttacg caaaatcagc aatttttcct
     8641 ggttctcact gttcactttt gctgcaaaat tattccgttt cgtttgtggg aataaccatg
     8701 gcagaagcat tttacatcct gataggattt ctgattatgg cggcgattat cgtcatggct
     8761 gtcctttacc tcgaaaacca ctcctgacga tcatattgtt ccccccggca tcgcattcac
     8821 cagacctgaa accgccttct gcgccgcctc tgcgctttgt gcaccgggta ccagaatcac
     8881 cttacggcac tcctcttcac gtttttcgaa aatctcatat ccccgggcgg cctcttcaaa
     8941 cggcatatag tgggtaacaa tttcttctgg tttcagtaat cctttctcaa ttaacggtaa
     9001 taattctccc agccatgcgt gaacgtgggt ctgtcccatt ttaaacgtca accctttatc
     9061 aaaggcgtcg ccaaacagga aaccgtgaat aaatccagcg tagacgcccg gtacgctaac
     9121 aatgccgcca cgcctgaccg ccgcaataca ctggcgcaac gctttaccgc tgctgccctc
     9181 cagtttcagg ttagtcagca ccgtttccgt ggtgctgcct ttcgcttcaa aaccgacggc
     9241 gtctattact gcatccacgc cccggtgacc tgccgtttgt tcaataattg actgtgccgg
     9301 atcgctatct tcatcgaaat taatcgggat cgcgccgtag cggtcagcgg cgaaacgcaa
     9361 gcggtaggga tgatgatcaa caacaaaaat ctgttccgca ccgagcaacc gtgcacaggc
     9421 gattgtcaac aatcccactg gaccggcacc atagactgca acgcttgaac cttgttggat
     9481 ctgcgcattt tttgctgcct gccatgccgt tggcagaata tcagaaagga aaagggcttt
     9541 atcatctgaa agcaaaggcg gtactttaaa cggccccaca ttccctttag ggacgcggac
     9601 atattccgcc tgtccaccag gaacgccacc atacaggtga ctataaccaa acaatgccgc
     9661 gggagctggt atctgttttt tattgagcgc agcgccttta cccgcattgg tattttcgca
     9721 ggcggcatat tgctgcattc gacagaaaaa acagtcgcca caagcaatga caaacggaat
     9781 taccacccgg tcgccttttt gcaaattttt tacgtccttt ccggtttcaa cgacttcccc
     9841 cataaattca tgaccaaaaa tatcgccatg tttaacctgg ggtattttgc ctcgataaag
     9901 atggaggtca gagccacaga tcgccgttgc cgtaatacgc agaataatat catctgcctg
     9961 ttcaatgccc ggatcgggaa cattttctac ctgaacgtga tgtgggccgt gataagtcaa
    10021 tgctttcatg caacctccag agacagttca aaaaagagtc agtctttaag ggtagctatc
    10081 acagcgggaa aacgggcggt accgcaggtt ttgggagaat tcctggtgtt cagtgagaat
    10141 atattaatca cagaaaccgt tcgtaaggaa aaagaaccag acaaatatca gggataaata
    10201 ttataatcat ggcgaatgat ttgttcatga ttaacaggga gaaagggtta tgtataagac
    10261 aatcattatg ccagttgatg tatttgaaat ggaattgagc gacaaagctg ttcgccacgc
    10321 tgaattcctc gcccaggatg acggagttat tcatctactt cacgtactac ccgggtcagc
    10381 cagcctgagc ctgcaccgtt ttgccgctga tgtgcgtcgt tttgaagagc atctgcaaca
    10441 tgaagcagaa gaacgtctgc aaacgatggt cagccacttc accatcgatc cttcccgcat
    10501 taaacaacat gtccgttttg gtagcgtgcg ggatgaagtc aatgagttgg cagaagaact
    10561 gggggctgat gttgtagtta ttggttctcg aaacccatcg atttcgaccc atctgttagg
    10621 ttctaacgcc tcgagcgtga tccgccatgc caatctgccg gtgctggttg tgcgttaatt
    10681 gccagaataa atatcccgcc ctgcccggag ccatcccggc agggcttttt aatgccataa
    10741 aaaagccgcc aggtttgacc ccggcggcta agcaattgca ggtgaatctt acttcttctt
    10801 atgcagtttt ggtgcgaatc aggtagtcaa aagcactcag agaggctttg gcaccttcgc
    10861 cagtggcgat gataatctgc ttgtacggaa ccgtcgtaca gtcacccgct gcgaacacgc
    10921 ctttgacgtt ggtttcgcat ttcgcatcaa tgataatctc acccatgcgg ttacgttcga
    10981 ctgcgccttc cagccagttg gtgttcggca gcagaccaat ctggacgaaa ataccggcca
    11041 gttcgatgtt gtgaatatcg ccgctgacac gatctcgata ttccagacca acgactttgc
    11101 tgccgtcgcc tttcacttcc gtggtttgcg cattcagaat aatgtcgacg tttttcaggc
    11161 tgcgcagttt gtcctgcaga acctggtcgg ctttcatttc tggcgcaaat tcgagcagcg
    11221 ttacgtgctc aacgataccc gccaggtcaa ttgccgcttc cacgccggag ttaccgccgc
    11281 cgataaccgc tacgcgtttg cctttaaaca gcgggccgtc gcagtgcggg cagtaggtca
    11341 cgcctttggt gcgatactga tcttcgccag gaacgttcat gttgcgccat tttgcaccgg
    11401 tcgccacgat aatgctgcgt gctttcagta ccgcgccaga agctgtttca atctgatgca
    11461 ggccaccttc aaccgctgcc gggatcagtt tgctggcgct ctggctgtcg atcacatcaa
    11521 cgtcgtattc atcaacgtga actttcagcg cacctgccag tttctgccct tcggtcttcg
    11581 gtacagaaat gtagttttcg atatcaacgg tatcgaggat ctgaccacca aaacgttcgc
    11641 ccatcagacc ggtacggatg cctttacgtg cggagtaaat tgctgccgct gcacccgccg
    11701 ggccggaacc gacgattaat acgtcataag catcacgctt gttcagctct tctgccgcac
    11761 gtttttccgc gccagtatca attttggcaa cgatttcagt caacgtcatg cggccctgac
    11821 caaactcttt cccgtttacg aacactgccg gaacgcccat cacgttgcga tcggtgattt
    11881 cgttctggaa ggtgccgccg tcaattgcag tgtgcttgat gcgcgggttc agtacgctca
    11941 tcaggttcag cgcctgcacc acgtccgggc agttgtggca agagagcgag taataggttt
    12001 cgaattcaaa atcaccgtca atatggcgaa tctgctccag cagagactgc gcttctttcg
    12061 acggatgacc accggtccac agcaacgcca gtaccagcga ggtgaactcg tggcccagcg
    12121 gggagcctgc aaaacgtggc ccctggttgg aacctgggtt ggtgatcagg aaagacggct
    12181 tacgcaccgg caagctgtta tcttctttaa aggtgacttt gtctgacagt tctgcgattt
    12241 cagccaacag ttccttgatt tctgccgatt tagcgctgtc atccagcgtg gcaattaact
    12301 caacaggctt ggtcaatttc tcaaggtaag ccttgagttg agttttcata tttgtgtcga
    12361 gcatgcttat ctcctgggct ttaaacatca tcatgcaagc ggcctctgcg gctacctgga
    12421 tgcagcttgc atcatggtgc ggggaagaaa atcgggtgcc gcagcacccg aagaattagg
    12481 tgaatttccg aagtatttcg cgccggagtt ggtagcgcga aagacgaagg aaatttagat
    12541 tttaccaacc aggtccagag acggagccag agttgcttca ccttctttcc atttagccgg
    12601 gcaaacttca cctgggtgag aagctacgta ctgtgctgct ttgattttac gcagcaggtc
    12661 agacgcgtca cggccaatgc cttcagcggt aacttcgatt gcctggatga taccctgcgg
    12721 gtcaacaacg aaggtcgcac ggtcagccag accttcatct tcacgcatgt tgtcgaagtt
    12781 acgggtcagg gcgccagtcg ggtcgccgat catcgcatat ttgattttag cgatggtttc
    12841 agagctgctg tgccatgctt tgtgggtgaa gtgagtatcg gtagatactg cgtatacgtc
    12901 tacgcccagt ttctgcagtt cttcgtagtg gtcagcaacg tcacccagtt cggtcgggca
    12961 tacgaaagta aagtcagccg ggtagaagaa gaagacgctc cagcggcctt cggtatcttt
    13021 ttcggtgatt tcgatgaatt cgccgttttt gaatgcctgg tttttaaaag gtttaatttt
    13081 ggtgttaatc aaggacatct atacttcctc cgtgttttcg atgagatgta aggtaaccaa
    13141 tttttgccga ttcggctaat gcgtttccat tatcaaatcg ataagtttta ccttacaacc
    13201 ttcgtaagac aactttgtga actttgcacc gccagcggcg ataaaaagta aaatggcccc
    13261 ctttccaggt gaccgtgtaa tctgaactac cctttggtat cttcagcgcc tgcctgcagg
    13321 ctgaggtgtt gcccttacaa atgcaacaac gacatggatt acaacaccct cataaacaaa
    13381 gggcaatcac ctgatctaag ctcttaccta tgacagtgat aggttatgcc ttttactcga
    13441 cttttgcact gactgaaaag gacaaattaa tgttaaaaaa gatactttta ctggctctgc
    13501 ttcctgcaat cgccttcgca gaggaacttc cttccccagt aaaagcgatt gaaaaacagg
    13561 gcattacaat catcaaaacg ttcgatgccc ccggaggaat gaaaggttat ctcggaaagt
    13621 atcaggatat gggcgtcacc atctacctga ctcccgatgg taagcacgct atctctggtt
    13681 acatgtacaa cgagaaaggt gaaaacctga gtaacacact tatcgaaaaa gaaatttacg
    13741 caccagccgg acgcgaaatg tggcaacgga tggaacaatc ccactggctc ctcgacggta
    13801 aaaaagatgc gccggtcatt gtctacgtgt tcgccgatcc gttctgccca tattgtaaac
    13861 agttctggca gcaggcgcgc ccgtgggtag attctggcaa agtgcaatta agaacattgt
    13921 tggttggggt tatcaagcca gaaagcccgg cgacagcagc ggcaattctt gcctccaaag
    13981 atcccgcaaa aacctggcaa caatatgaag cctctggtgg caagcttaag ctaaacgtgc
    14041 ctgcaaacgt aagtacagag caaatgaaag tgttaagcga taacgagaaa ctcatggacg
    14101 atctgggggc aaatgtcact ccggctatct attacatgag taaggaaaat acgctacaac
    14161 aggccgtggg gttgcccgat cagaaaacgc ttaatatcat tatggggaat aaataaaagg
    14221 taattcatgg atggttgaac tatatccctg cttttcaacc atcctaaaaa agcatgaatt
    14281 gctcgctctg ccctaagtgg ctgtgctatt ttagaaaaac ttaatcctga gttaagcaaa
    14341 aaaacataat caataaaaat atatacgcaa aaaacattga ttaagtgaat atatcatgga
    14401 agaaaaatat aaccggagta gtgtatggcc aatctctacg acttgaaaaa gttcgactta
    14461 aatcttcttg tcatatttga gtgtatttac cagcatttaa gcattagcaa agctgctgaa
    14521 tcactatata tcaccccatc agccgtgagc cagtcgttac aacgcttacg tgcgcaattt
    14581 aatgaccctt tattcattcg ctcaggaaaa ggtatcgcgc cgacgacaac agggttaaat
    14641 ctgcatcacc atcttgagaa aaatctcaga ggccttgaac aaacaattaa tatagtcaat
    14701 aagtccgaac taaagaaaaa ctttattatc tacggccctc agctcatttc ttgctctaat
    14761 aatagtatgc tgatccgctg cctacggcaa gattcctctg tcgagattga gtgccatgat
    14821 atcctcatgt cagcagagaa tgctgaagaa ctgttggttc accgcaaagc cgatctggtg
    14881 atcacccaaa tgccagtgat cagccgttcc gtcatttgca tgccattgca tacgattcgc
    14941 aatactctaa tttgtagtaa taggcatcca cgaattactg acaatagcac ttatgaacaa
    15001 attatggcag aagagttcac ccaattaata tctaaatcag caggggttga tgatattcaa
    15061 atggaaatag acgagagatt tatgaatcgc aaaatctcat ttcgtggttc ctctttgctg
    15121 acaatcatta atagcattgc agttacagat ttactgggaa tagtccccta tgaactttat
    15181 aattcctacc gtgactttct taacttaaaa gagataaaac tagaacatct gttaccttca
    15241 ataaagcttt acatttccta taataaatct tctttaaata atttggtgtt ctcccgattt
    15301 attgaccgtc taaatgagag tttttgatat ataactacag acagctacta taactttcat
    15361 ccatttattc acagcgcgaa tgaaaaataa aacactctgc tttacagtgt ttactttata
    15421 attaacagac ttatctcact gatcaccctg taacgttcag agagcgtctt cagatgtcta
    15481 tttataaaat tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctgga
    15541 cactcaacac cctaccccgc gtttgtgtct cattttctgg cggcaaagac tctggtttaa
    15601 tgctgcatct gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta
    15661 tcgactggga ggcgcaattc tcttgcacca ttaactatgt tcagtccctg cgcgagttgt
    15721 acaccgatgt catcgaagag ttttactggg ttgcgctccc gcttacgacg caaaattccc
    15781 tttcacaata ccaacccgaa tggcagtgct gggaacctga tgtcgaatgg gtacgtcaac
    15841 ctccccaaga tgcgataacc gaccctgact ttttctcctt ttaccagcca ggcatgacct
    15901 tcgaacaatt tgtacgtgag tttgccgaat ggttttcaca aaaacgtccg gcggcgatga
    15961 tgatcggcat ccgtgcggat gagtcctaca accgttttgt cgccatcgcc agtttaaata
    16021 aacaacgttt tgccgacgat aaaccctgga ctacggccgc accaggcggt catagctggt
    16081 acatttaccc catttacgac tggaaagtgg ctgatatctg gacctggtat gcaaatcatc
    16141 aaagtctctg caacccactg tataacctaa tgtatcaggc aggcgttcct ctgcgtcata
    16201 tgcgaatttg cgaacctttt ggcccggagc aacgacaagg attatggctc tatcacgtta
    16261 tcgaaccgga tcgctgggct gctatgtgcg cacgagtcag cggggtaaaa agtggcggca
    16321 tttacgccgg acatgacaat catttctatg ggcatcggaa aatcctcaag ccagaacatt
    16381 tagactggca agaatatgca ttattgctgc tcaatagcat gccggaaaaa acagctgagc
    16441 attaccgcaa taaaatcgcc atttatttgc actggtatca gaaaaaaggc atcgaggttc
    16501 cacaaaccca gcaaggggac attggcgcga aagatatccc ctcctggcgg cggatatgca
    16561 aagttttact caataacgat tactggtgtc gggcattatc atttagcccc acaaaatcga
    16621 agaactatca gcgttataac gaacggataa aaggaaaacg tcaggaatgg gggatactat
    16681 gcaacaacga ttaacgcagg atttaacgca atttctcgcc agcttgccgg aagatgatcg
    16741 catcaaagcg attaatgaaa tccgcatggc gatccatcag gttagcccct ttcgtgaaga
    16801 gccagtggat tgcgttctgt gggttaaaaa cagccaactt atgcctaatg attacaaccc
    16861 aaacaacgtg gcaccacccg aaaaaaaact gctgcaaaaa tccattgaaa tagatggttt
    16921 tacccaaccc attgtggtca cacatacgga taaaaatgcc atggaaatcg tggatggttt
    16981 tcaccgccat gaaattggta aaggctcatc atcattaaaa ttacgcttaa aaggttattt
    17041 gccggtgacc tgtctggaag gtacgcgtaa tcaacgcatc gccgcgacta ttcgtcataa
    17101 tcgcgctcgt ggacgccatc aaatcaccgc catgtcggaa atcgtccgtg aacttagcca
    17161 gttgggatgg gacgataata aaattggcaa agagctggga atggacagcg acgaagtgct
    17221 gcgcctgaag caaattaacg gcctgcaaga gttgtttgcc gaccgtcaat attcccgcgc
    17281 ctggacggtt aaatagctaa agctggcgca ggcgttcagc tgctgccagc aacgtcgatt
    17341 ccttcttggc aaaacagaga cgaatcagtt tatgtgggaa gggatcggcg caaaacaccg
    17401 acagcggaat cgccgctacg ccgtgctcct gcgtcagcca ctggcaaaac tcaacatcat
    17461 ccagggtaga aaccgcgctg taatccacca gcaaaaagta tgtaccttca cacggtaaaa
    17521 tctccagccg gctttcattt aaagcattca ccagaatatc gcgcttctgg cgataaaagt
    17581 ccggtaacgc aagataatgc tcaggttctg cacgtagcat atcagcaagc gccagctgtg
    17641 ccggggtatt caccgaaaag gtcagatact gatgtacctt gcgaatttcg gcgctgatgg
    17701 gcgctggcgc aacacaataa cccactttcc agccggtcat atgataggtc ttgccaaatg
    17761 aagaaaccgc cactgcccgc tcacgcagct gcggatgcgc cagcacactg gcatggccct
    17821 gttgtgaaaa gttgatgtgc tcgtagactt catcgctaat gacaaaaatc tcgtgcccgg
    17881 cgatcgcctg ccacaaagcg gcgaaatcag cctgctgcca gatagttgca ctggggttat
    17941 gcggagtgtt gaggatcacc agtctggtgc gctcgcttaa taatgcggca aattcctgcc
    18001 agtcaacgcg aaaatgcggt ggttgcagtg ccatacgctt cactattccc ccagaaagcg
    18061 cgatggcggg ggagtaactg tcatagctgg gatcaaaaca aatcacttca tcgccattgc
    18121 gcaccagtgc ggtaatcgcc gcgtataacg cttccgtcgc ccctgccgtt acggtgatat
    18181 cgctatcggc atctggttga tagccataca aacgttccgt tttctgagca atcgcctcgc
    18241 gcaaggcctg cacgccggtc atgggcgcgt attggtttgc cccctgggca acgtggtacg
    18301 ccagccgctc ctgtaaatag cgcggaccat caaaatcagg aaagccttgc gacaggttaa
    18361 tcgcctggtg ttgctgcgcc agcgcgctca tctgggtgaa aatagtggtg ccaagttgtg
    18421 gaagtttgct ttgtggaatc agagggttat ttgtcatttt tattgtacct gacgaagttg
    18481 cgtgttgaag gcactataac acgatgctag tatttggcaa tcaagacgtt tagatgtcta
    18541 aataaaacaa taaggacaac acaacatgcc tcacaatcct atccgcgtgg tcgtcggccc
    18601 ggctaactac ttttcacatc caggaagttt caatcacctg aacgattttt tcactgatga
    18661 acaactttct cgcgcggtgt ggatctacgg caaacgcgcc attgctgcgg cgcaaaccaa
    18721 acttccgcca gcgtttggac ttccaggggc aaagcatatt ttgtttcgtg gtcattgcag
    18781 cgaaagcgat gtacagcaac tggcggctga gtccggtgac gatcgcagcg tggtgattgg
    18841 cgtcggcggc ggcgcactgc tcgacaccgc gaaagccctc gcccgccgtc tcggtctgcc
    18901 atttgttgcc gctccgacga tcgccgccac ctgcgcagcc tggacaccgc tctccgtctg
    18961 gtataacgat gccggacagg cgctgcatta tgagattttc gacgacgcca attttatggt
    19021 gctggtggaa ccggagatta tcctcaatgc accgcaacaa tatctgctgg cggggatcgg
    19081 tgacacgctg gcgaaatggt atgaagcggt ggtgctggct ccgcaaccag aaacgttgcc
    19141 gctaaccgtg cggctgggga tcaataatgc gcaggccatt cgcgacgtct tgttaaacag
    19201 tagcgaacag gcacttgccg atcagcaaaa tcagcagtta acgcaatcat tttgcgatgt
    19261 ggtggatgcg attatcgccg ggggtggaat ggtcggtggt ctgggcgatc gttttacgcg
    19321 tgtggctgcg gcccatgccg tgcataacgg tctgactgtc ctgccgcaaa ccgagaaatt
    19381 tctccacggc accaaagtcg cttacggaat tctggtgcaa agcgccttgc tgggtcagga
    19441 tgatgtgctg gcgcaattaa ccggggcgta tcagcgtttt catctgccga ctacgctggc
    19501 ggagctggaa gtggatatca ataatcaggc ggagatcgac aaagtgattg cccacacctt
    19561 gcgcccggtg gagtccattc attacctgcc agtcacgctg acaccagata cgttgcgtgc
    19621 agcgttcgaa aaagtggaat cgtttaaagc ctgacgtgca aatttagcag cagcgcgcgc
    19681 cgccttttcc accgtagcgc gcgtcctgcc gctcacggaa aaactcctcg taggtcatcg
    19741 gcgtttgatc gggatggtta acccgcatat gttcgacata gttgtcgtaa tcaggcatac
    19801 caatcatcag cttcgccgcc tgacctaaat attttccggc ttttgccagt gaatcaaaca
    19861 tcttggcctc cgctaaccat gttgctgaaa gagtgccgga tgcggcgtga acgctttatc
    19921 cggcctacaa atgctatcaa cgcactcggt cagtcccctc gccccatcgg ggagagggtt
    19981 agggtgaggg ggaaccctcg gcactatgcc aacattcgcc aacatccccc ctcactctga
    20041 ctttagtgcg cacctttcgc ctgcgctacg atctcctcaa cattttccgg cattggctca
    20101 tacggcgttt ctttcgccgt tggcttcggc tctttcaatg ccgcaagtgc cgtcttaatc
    20161 gagaacagtg ccagaaccac cacgaccacc ataaagaaga tggttaaccc ggcatccaga
    20221 cggttgttga acaccagttg cgccagctgt gactcagtat actgcgacgg aatattgccg
    20281 ctgtcgatca ttgcctggaa cttgttagca atggccagga agccgacttt cgcatccggg
    20341 ctaaacgctt tctgccagcc tgcggtcagg gtacaaatca gtagccaggc cgttggtacc
    20401 agcgccaccc aggcgtaacg ttgacgtttc atcttgaaca acaccacggc acagagcatc
    20461 agcgccatcc ctgccagcat ctggttggca ataccaaaca gcggccacag agtgttaata
    20521 ccgcccagcg gatcgaccac gccctgatgg aggaagtagc cccacgccag tacgcacagc
    20581 gccgttgcca gcaggttagc aggcagtgaa tcggtccgct tcaggccagg agacaccacg
    20641 cccagcagat cctgcaacat aaagcgcgca gcacgcgtac ccgcatctac cgccgtcaga
    20701 ataaacagag cttcaaacag aatggcgaag tgataccaga acgccacatc catcatacca
    20761 cccagcgcac cgtgcaggat gtaagccatc cccaccgcca acgtcggcgc accgcccgca
    20821 cgggaaatga tcgactgttc acccacttcg ctggcaatct ggttgagcgt atctggtgta
    20881 atgctaaagc cccagctact caccacctgc gcggcagaag cgaccacatc cgccgtccct
    20941 gccggagcca gcaccgccat tgggctgttc atggcaaagt aaacgcccgg atcgatgata
    21001 caggcggaaa ccagcgccat aatcgccacg aaggattcca tcaacattcc accgtagccg
    21061 ataaaacaag cctgcccttc gttcgccagc atcttcggcg tggtgccgga agagatcagc
    21121 gcatggaagc cagacaccgc gccacaggcg atggtgataa acaggaacgg gaacaggtta
    21181 ccggtccata ccgggccagt gccatcgaca aatttggtca gcgcaggcat ggtcagcgtc
    21241 gggcgcataa tcaaaatgcc taccgccaga ccgacgatcg tcccgatttt caggaaggta
    21301 gagaggtagt cacgcggggc cagcagcaac cacaccggca gcaccgccgc cacaaaaccg
    21361 taacccacca gcatccaggt cagctgcacg ccggtaaagt caaagtacgg tgcccaggtc
    21421 ggactttctg ccacccagcc gccagagata atggcgaaaa tcaggaatac cagaccgatg
    21481 accgacactt caccaatacg ccccggacgc agatagcgca ggtagatccc cataaacagc
    21541 gccagcggaa tggtgaacgc aacggtatac gttccccacg ggctatgagt cagggctttc
    21601 accacgatca tcgccagtac tgcaaggata atgaccatga tcataaagca ggccaccagc
    21661 gctatcaccc cggcggttgg ccccatctct tctttgacca gctcacccag cgatcgaccg
    21721 tcacggcgcg tagaaacaaa cagcaccatg aaatcctgca ccgcaccggc gagaaccacc
    21781 ccggcgagca gccagatcat ccctggcagg taacccattt gcgccgccag taccggccca
    21841 accaacggac ctgctccggc aatggccgca aaatggtgac cgaacagcac tttcttgtcc
    21901 gtcggcacat agtccagccc gtcgttatgg cgcaccgctg gcgtcatacg cgtcgggtca
    21961 accgccagca catttttggc gatatacagc ccataaaaac ggtaagcgat cagatagata
    22021 cagaccgacg ccaccacaat ccacagcgcg ttgatctgtt ccccacgatt taaagcaatg
    22081 tatcccagag caaatgctcc cattacagag agcactgtcc agacgaggta tttccctgat
    22141 ttgttcatag ttgttatccg tgtgcgtgtc catagatatg ttacattttg tttctataac
    22201 atctttgtac gatttaacaa cacggtaacc atgtaaatcg gagttgaaac caatatttaa
    22261 cttaacaatg ttaactcgat cactccgtta accgagtttt cctgcaatct catttaattc
    22321 tgtcgggctc atcccaaaac tgccgtaccc agccgacaag tgcagcaacg ccgcccctgt
    22381 tcatcgaaaa cgacgatttc ccagctctga ttttgccgcc caagatgcag cggctggcag
    22441 acgccgcgta ccttgccttc agacaccggg cgatgatggg tggcgttaag ttccgtaccc
    22501 accacacact gcccgtcacg ggtcatcata aatccggcca tcgatcccag cgtttcagcc
    22561 agcgccgctg acgcgccgcc atgcagcagg ccaaacggct gatgagtacg ggtatcaacc
    22621 ggcatttcgg cttccagcac atcatctccc agacgggtat acacaattcc cagatgcgcc
    22681 accattgtgt tatcgctggt ggcgttcagt tcgtcgagcg ttaaatggcg tttccagatc
    22741 atgcttatgc ccccagcgtt gagccgccat cgaccacaat atcctgtagg gtgatatggc
    22801 tggcgaggtc agaggcgagg aacaaaatcg tgttggcgat ctcttgtgga cgggcgattt
    22861 tccccagcgg aatgccgagt ttaaactgct cgccaaagcc gcgaatacgc tgttcttcgg
    22921 cgtcatcgct cacccacagc gtgcgttgca tatcggtgtc ggtggagcca ggcgaaacca
    22981 cattacagcg cacgccgcta accgccagtt ccagcccgac gctcaacgcc aggcttttca
    23041 gcgccgcttt cgatgcgcca taagcactca taccaatacg cggcgtgtgt gcggcgtcgg
    23101 acgccacagt gacaatcgcc ccgccccgct gacggcgaaa ctggttcatg gtttgctgaa
    23161 acaggttaaa cgcaccgccg acgttaaccg caaaagtctg ctgccagtcc tctttgctca
    23221 gttggtctgt cgcgcccatg cgtaaaattc ccgccgcatt gaccagcgcg tccagtcgct
    23281 ccgtttcagc taacagtcgc tgacacactt gcgccacctg cgcagcgtcg gcaacatcca
    23341 tcacttcggt cgcaaaggga tattgctcct gagtgaacgc ttgatcaaaa cctgtaactt
    23401 tcgctcccgc ctcaacaaac gccagcgccg tggcgtagcc gataccttta cctgcgccgg
    23461 ttacccagac atttttaccg ctgaaatcca ttatttcacc tcgcgggaga gtagcttcca
    23521 ccaggcgtcg atggtcgggt ttttcgccag catgacaaaa tcgatatcac catgcacttt
    23581 gcgccagcgc gccgccagcg ccatcatgcg caccgaatcc agaccgtagt cgatcaggtt
    23641 gtcgtcatcg aacggttcat cggactcgtc cagcaacggc aggataacct cacgcagcgc
    23701 cgctttgctg gcggggacag gtgctggcag taattcttca gtcatcacca cccggccaga
    23761 ccgcccggcc acatatttca gcgacatcaa atgctcgtca cggctgaaat cggccagcgc
    23821 atccgccacc ataaacggtt taatatcgcg cataaatgcg tcggttgcgg tggtcataca
    23881 gccaatgtgg gcatataccc cggtaataat cagctggtta cgtccactct ctttgagcat
    23941 ttgctccagc ggagaacgat gaaacgcgct gtagcgccac ttcaccagca ccgtgtcgtc
    24001 ggcatctggc gtcaggcgat ccaccacctt ttgctgttcc ggcgaacggg tcaggcccgg
    24061 cccccacata tcattcaaca gcgcccgatc ttcatcgctc tgctctttcg gctgggcggt
    24121 gtaataaacc gggatattgt gctgtttgca gtagtcgcgc agcgcagcaa tattcgcgat
    24181 cacctgctcc atcatcgggc agttctcgcc ccagaagctg acaaaatagt cctgcatatc
    24241 atggattaac aacgcggcac gttgcggttc aaaggcccag tcaactttat tctgcggaat
    24301 atcgtgagac tccggcagtg cgtaagcctg taattttgga atagccatcg tgttctctcc
    24361 ttcaggctga tgcgcgtgac gccagccact gacgtaattg ttttttatcg actttcccga
    24421 ccgccgtcag cggaagtgaa tccacacact ccacgcgatc cggtaattta aattcggcaa
    24481 taccctgttc acgcaggaaa cgacgcacct gcaccgcgcg cagcggctct tttaccacca
    24541 gataagcaca gcttttttcg cccatcagct catcttccat gctcaccagt gcggcataga
    24601 tcaccgccgg atggcgcagc agcaggtttt cgatctcttc ggcagcgatc ttctcgccgc
    24661 cacggttgat ctgatctttc tcgcgcccct gcacggtgat gtaaccctct ggatcaatag
    24721 agatcagatc gccggaacag taaaaaccgt tggcatcaaa ggcactggca ttgtgctgcg
    24781 gacttttgta ataaccgcgg aaggtgtacg gcccgcgcgt catcaggcgt ccaacttccc
    24841 cttgcggcag tggatttcct tcagcatcag caacccatac ttcatcgtcc ggacacattg
    24901 ggtaaccctg ggtatggata attttctccg cgctatcatc aagacgggtg tagttcacca
    24961 gcccttccgc catgccaaac acctgctgca actggcagcc aatctcagcg ggaatacgcg
    25021 ccgcaagggt ggcagaaaga cgtgcgccgc cgacctgtaa cagtttcagc gaggcaagct
    25081 gcgcccggct ttcaccttcg gtcagcgcct gcaaccacag gctgactgcc ggtggcacca
    25141 gcgcggtgac gttcacctga tgtttttcaa tcaatgggaa gcaaagcgtg gcgctgggat
    25201 cggccgccag aacaaccgtt cctccggcaa gaaagacgcc cagcgatccc ggcgaactca
    25261 tggcgtagtt atgagccgcc gggatcgcgc acaggtagcg tgtctgttgt gtgaactgac
    25321 aaatctcgac gctacgacgc acgctgtagt agtagtcgtt atgagtgcgc gggatcagtt
    25381 tcggtgtgcc ggtggtgccg ccggaaagct ggaaataggc cacttcatca gcaggtgatg
    25441 gcgtggcagt aaaatcctca gccggatggt taatcgcatc ctgcaagtta tgctcaccgc
    25501 tgtcgttgtg cagttgcacc acgcgaatgg aggaatgttc tgtgacgaaa gtattgagga
    25561 aatcatcccc gctaaacagc gcatgttggc gatcggcaat cagcaatgcg ggttcaatct
    25621 ggctggcata ggcgttcagt tcactacgct gatggctgaa cagcgccagc accggcgcaa
    25681 cgcccagttt cagcagcgcg aaaaaggtaa tatacaattc agcgacgtta cccagttgta
    25741 ccagcgcggt ttcaccaggt ttaatgccct gacggcgtaa actacacgcg aggttatccg
    25801 ccgcctgatt cagctcccgg taactcaact gtcgctcgcc gtcgataacc gcgatgctgt
    25861 cactcgcagc gtggcgagtc agaatgtcgg tcagcggcaa atcctgccag tagccttttt
    25921 cccgatagcg acgggcaaac tcttccggcc agcgggtgaa tggaatgctc atcctcgctc
    25981 cttaatgcaa tccaaaaacg ttcaacatgg tagaaagttt gacgcctgtt tcgcgccact
    26041 cacccaacgg tgacgacgca ggcacaatcc ccgctccggc aaacagacgc acctgatttt
    26101 cccgcagctt cgcgcagcgg atggtcacca cccattcgcc gttaccttcg ctgtcacacc
    26161 aacccacaat gccgccaaac agttcgcggt cgaatggctc cagttcagca ataacctggg
    26221 tcgcggcctg atgcgggaaa ccgctcagcg cgggggttgg atgcagcaga caggccagag
    26281 tcagtgcgtt ttcttgcgaa ttcgctttac cttcaaaggg agttgcgaga tgccacagcg
    26341 tcggcgtggt aatcaattgt ggagaggagg gaacgtgtaa ctcactactg cgttcgcgca
    26401 gtacctcttt catcgcctga gtcaccagtt catgttcatg gcgatctttt tctgacgcca
    26461 gcagacgatt acccgcttcg cgatcgagca cttcatccgg ctgacgacgc gcggaaccgg
    26521 ctaacggaat ggagctaaaa cgctcgccgt ctttacgtag cagcagttcc gggctggccc
    26581 ccagcaggac gccaccatca gccagcggga catggaagtt gtaactaacc gggttttgcg
    26641 caatcaaccg ttccagcaat acgccactat caatggcggc gtcagtggtg atatcaatca
    26701 atcgtgacaa caccactttg tcgacctgcg gcgtggcggt aagtgcggcg gcgcgggcaa
    26761 ccatctgttc aaacgtggtt tgttcaggaa ttgcctggcg ttccaccacg ttcagcgact
    26821 ggctgcgggt aaaacggcgg gctgaggtct gtttttcctg gcgggagaac gactgccagg
    26881 attcgggaat atacagcgac gaaggctgac gtggatcgaa gggaatcgcc ccgaccatca
    26941 ccggattttt gatgccctgc gctttggcat cggcaaacag cgcggcgagt ttttgctgga
    27001 agggactgtc gggcgaatcc ccgttcacag ccggttcatc gaagcgggcg aaacatcctg
    27061 acgtcgtaaa actgcggtac ggcgacataa agaaaaagcg attgggcgca agtgttgcca
    27121 tggtctgctg tacttcctca gccagtgacg tatccatatc atcctccaca aaatgataaa
    27181 ggctttaata atgattatca tttatatttt cggtcgctaa cctaaaagca aacgcgcact
    27241 atgtcaactc ttgaggtaac gcaatctgca actcggggtt gcatctggtt gtggttacaa
    27301 tgaaaatgag aagcattatt gatggattcg cataagcgca atgtgatgtc ctgcgccgtt
    27361 ctgccccctc tcccttccag ggtgagggct ggggtgaggg ttaatgttcg caccagtgct
    27421 ggctgttccc ctcaccctaa ccctctcccc aaaggggcga ggggacggat tgtgcgcttt
    27481 gtcgaatttg tcattacgcc cttaacctta ttaataacag gaagctgatt tgtgagactc
    27541 gccccgctct accgcaacgc ccttctatta acaggacttt tgctttcagg aatagccgca
    27601 gttcaggccg ctgactggcc gcgtcagatt actgacagcc gtggcacaca tacactggaa
    27661 agccagccgc agcgtattgt ttccaccagc gtcaccctga ccggctcact gctggcgatt
    27721 gatgcaccag tgatcgccag cggcgcgacc acgccaaata accgcgtcgc ggatgaccag
    27781 ggctttttac gccagtggag caaggtggcg aaagaacgca aactgcaacg gctctatatc
    27841 ggcgaaccga gcgccgaagc cgttgccgcg caaatgccgg atctgatttt aattagcgca
    27901 actggcgggg attcggcgct ggcgctgtat gatcagcttt ccaccatcgc cccgacatta
    27961 atcatcaatt acgacgacaa aagctggcag tcgctgttaa cgcagcttgg cgaaattacc
    28021 gggcatgaga aacaagcggc agagcggatt gcgcagtttg ataagcaact ggcggcggcg
    28081 aaagagcaaa tcaaattacc gccgcagccg gtcactgcca ttgtctatac tgccgctgca
    28141 cacagtgcca atctctggac gccagaatca gcacaagggc agatgctgga acaactcggc
    28201 tttacgctgg cgaagttacc cgcagggtta aatgccagcc aaagtcaggg taaacgccat
    28261 gacatcattc agcttggtgg ggaaaatctg gctgcagggt taaatggcga gtcactattc
    28321 ctgttcgccg gtgatcagaa agatgccgat gctatttatg ccaatccact gctcgcgcac
    28381 ctgcccgcag tacaaaacaa gcaggtttat gcgctgggaa ccgagacgtt ccgtctggat
    28441 tactacagcg ccatgcaagt gctggaacga cttaaggcgc tgttttaagc attaaccgtc
    28501 agacgctgtc acctgcggcg gcgtctggcg aaaacgtcgc aactccacca gcaccagcaa
    28561 taacaacacg ccgataatca acaaaccaaa accgctcgcg cttgcggaag caaccggtgt
    28621 catcatcgct cccaaaccgc ccagcagcgc cgcgcctatg gcatcgcccg tcacgttttg
    28681 tgccgtccac aaaccgttaa tccgccctaa catcgcttcc ggggtttgcg tttgcagcat
    28741 tgtgtattgc agcaacgagc tgaccgcact caaccagccg aacagcgcca gacaaaccac
    28801 gcctaaaatc cacatcggca tcaggccaaa cagaccaatg gcgaggaacg atcccagcgt
    28861 ggagagcagc atcaataacc ctggtcgcgc gctatgcgcc agcttcccgc tggttaacgc
    28921 gccaatagcc gcgccgagcg ggatcgccgc gtagagaaaa ccaatctttg ccgctgacat
    28981 ctgccagttg tcagccagcg ccggatacag tacccgcacc gcgctcgcca tcgtcaataa
    29041 cccccccagc agcgcaatgc ctcccaccag cgggctggcg agcagaaaac gaaatcctgc
    29101 cagtaatgat ttcaacggat gctcacgcgg ctgcggtggc ggtggcagtg ccggaaggct
    29161 taacaacggt agcaaggtaa taaacgtgcc cgccgccgcc agtccgtagt tccaggccac
    29221 accgccagtc gccagcagta aaccgccaat catgggcgaa atcaccgacc ccagacgcac
    29281 ggtcaacatg gtgatcgccc cggcctgcat taagttttca cgccctacca gtgcaggtgt
    29341 cgccgccagt agcgccgtaa cgccaagtga tgcgaaaaaa ccatcccata aaccaagtaa
    29401 atagattgcc agcaatgacg gttcaggcag cagtgcatta aggcacagtc caatgaagcc
    29461 aatgccacag gtgccgcgcg ccagcaaaat cacttttttg cgttcatagc gatccgccag
    29521 cacaccgccg accatcaggc caacaaacat cgcgccgccg gtcagcgtca ccgaaagccc
    29581 cacctgccag gtagaatgtg tcatcatctg gatctgcacc ggcaccgcga cgccgagcaa
    29641 acccagagaa acaatagaga tgaaacgagc gaggaatact gcgcgaaacg ccgggtgcgt
    29701 tttcaacagg ctgaggttaa gcagccagga ttgtttattc attacaatgc cttgccatca
    29761 aatattttaa atacctattc ttaggctgca catgctaaca tatccaaata agatcgataa
    29821 cgataattaa tttcattatc atggaagttc gtatgtctgg ttctgttgcc gtgacacgcg
    29881 ccattgccgt gcccggattg ctgttattac tgattatcgc gacggcatta agtctgctca
    29941 ttggggcaaa atcactcccc gcttccgtag tgctggaggc cttctccggc acctgccaga
    30001 gcgccgactg caccatcgtg ctcgacgcgc gtctgccgcg tacccttgcc ggtttactgg
    30061 caggcggcgc gcttggcctt gccggggcgt taatgcaaac cctcacccgt aacccacttg
    30121 ccgaccccgg cttgcttggc gtgaacgccg gagccagctt tgccattgtg ctgggtgcgg
    30181 cgctgtttgg ttactcttcc gcacaggaac aactggcgat ggccttcgcc ggggcgctgg
    30241 tggcctcgtt gattgttgcc tttaccggca gccagggcgg cgggcagtta agtccggtgc
    30301 gtttaaccct ggcgggcgtg gcgctggcgg cggtgctgga aggactgacc agcggcatcg
    30361 ccctgcttaa tcctgacgtc tacgatcaac tgcgtttctg gcaagccggt tcgctggata
    30421 ttcgcaatct acatacctta aaagtggtgc tgatcccggt gctgatcgcc ggagcaactg
    30481 cgctattact gagtcgcgcg ctgaacagtt tgagcctcgg cagcgacacc gcgacggcgc
    30541 tgggcagtcg cgtggcgcgc acacagttga ttggtctgct ggcgattacc gtgctttgtg
    30601 gtagtgcgac ggcagtagtt ggcccgattg cctttattgg cctgatgatg ccgcatatgg
    30661 cgcgttggct ggtgggtgcc gatcatcgct ggtcgctgcc cgtcacgcta cttgctaccc
    30721 ctgccctgct gctgtttgcc gatatcatcg ggcgggtgat tgtgcccggc gaactgcgcg
    30781 tttctgtggt cagtgcgttt attggtgcac cggtgctgat cttcctcgtg cgacgtaaaa
    30841 cgcgaggtgg tgcatgattt acgtctctcg ccgattactc atcacctgtt tgctgctggt
    30901 ttccgcctgt gtggttgcag gtatctgggg attacgcagc ggtgccgtca cgctggaaac
    30961 ctcgcaggta ttcgccgcgc tgatgggcga tgcgccgcgc agtatgacga tggtggtcac
    31021 cgaatggcgt ttaccacgcg tgctgatggc gctgttgatt ggcgcagcac tgggcgtcag
    31081 tggcgcgatt tttcagtcgc tgatgcgtaa cccgctcggc agccctgacg taatgggctt
    31141 taacaccggg gcgtggagcg gcgtgctggt ggcgatggtg ctgtttggtc aggacctgac
    31201 ggctatcgcg ctgtcagcaa tggtgggcgg cattgtcact tcgctgctgg tctggctgct
    31261 cgcctggcgc aacggcatcg acacctttcg gttgattatt atcggtatcg gcgttcgcgc
    31321 catgctggtg gcctttaata cctggctgtt gctgaaagcg tctttagaaa cggcgctaac
    31381 agcaggtttg tggaatgccg gatcgctcaa cggcctgacg tgggcaaaaa cctcgccttc
    31441 cgcacccatc attatattga tgctcattgc cgccgcctta ctggtacgcc ggatgcgctt
    31501 gctggaaatg ggcgatgata ccgcgtgtgc gctgggcgtc agcgtcgaac gttcgcgtct
    31561 gttaatgatg ctggttgcag tggtgcttac cgctgcggca acagcgcttg ccgggccgat
    31621 ttcctttatt gctttagtcg caccgcacat tgcccgacgc attagcggca ccgctcgctg
    31681 ggggctaacc caggcggcgc tatgcggggc gctgttactg ctggcggccg atctctgcgc
    31741 ccaacaactg tttatgccgt atcaacttcc ggttggcgtc gttaccgtca gcctcggcgg
    31801 tatttacctt atcgtcttgt taattcagga gtctcgcaaa aaatgaccga atcagtagcc
    31861 cgtttgcgcg gcgaacagtt aaccctcgga tatggcaaat ataccgttgc ggaaaatctg
    31921 actgtagaaa tacctgatgg tcacttcacg gcaattatcg ggccaaatgg ctgcggtaaa
    31981 tccacgttac tgcgtacctt aagccgcctg atgacgcctg ctcatgggca tgtctggctg
    32041 gatggcgagc acattcaaca ttacgccagt aaagaggttg cacgccggat tggtctgttg
    32101 gcgcaaaatg ctaccacgcc gggcgatatc accgtgcagg agctggtggc gcgtggacgt
    32161 tatccgcatc aaccgctgtt tacccgctgg cgcaaagagg atgaagaagc ggtaacgaaa
    32221 gcaatgcagg ccacgggaat aactcatctg gcagatcaaa gcgtggatac cctttctggc
    32281 ggacaacgcc agcgcgcgtg gatcgcgatg gtgctggccc aggaaacggc aattatgctg
    32341 ctcgacgaac ccacgacctg gctggatatc agtcatcaga ttgatttgct ggagttgtta
    32401 agcgaactga accgcgaaaa aggctatacc ctggcggcgg tgctgcacga tcttaatcag
    32461 gcctgtcgtt acgccagcca tttgattgca ttgcgggaag ggaaaattgt tgctcaggga
    32521 gcgccgaagg agattgtcac tgctgaactg attgagcgca tttatggtct gcgctgcatg
    32581 atcattgacg atccagttgc cggaacgccg cttgtggtgc cgctcggacg aacggcacct
    32641 tcaaccgcaa atagttaaac taagtggtct gccatcatgg catcctgttt tctggatgcc
    32701 atcgcatagc gcaataacac gctaccacaa gccaccatcc cgccgatcag cgcggaaagg
    32761 atcacaataa tcgccttacc cggaccgtct tttttcactg gcaatgacgg gcttaactga
    32821 tatttaaacg gcgtaaaatt cacatcgttg atatttgctt ttgttaactg ctcaacaaga
    32881 tactgacgat tacgtaattc accgttcagt tccgcaacgt cagtgaccgc tttttcaatt
    32941 tccagtttgc gttcaatacc gtctgcgccg agagaaatag agaaatcggg gtcatcttta
    33001 acggcctgac cattactgta tacgggcttt ttaattcccg ccgcgttagc aatgtcgagt
    33061 gaataattga ggcgctgaat gtttgcatca agttgatttt tcgttttaat gcgatcctga
    33121 gccagttttt ctttttcaaa ctgggttttg atttccagtt tattacggac gttttctagt
    33181 gactctttca ccaccaacgc agagatataa tcgatatacc cgctcaaaac ggtctgcgcc
    33241 tcttcactgg ttggcgcggt aaaacttagc gtccaggagg tatacagtga cggttcatct
    33301 tttttcttac tggcattgtc atcaaccgct ttcatttttt cgctcaatgc aacaatagcg
    33361 cgatgcaaat ccagttcatc gattttcgcc tcttttaatt ggtccatcac ataaggtgat
    33421 gaacgcaggt actcttccag caagctaacc gactgaaact tcttgataaa caggttaaat
    33481 gcttctgtac gatcaatttt gatatccaga tccagcactc gaagcttagt gaatgttttc
    33541 tccagctctt gccactgaac aggttctgga ggcgtgacaa ccgccgcgct ggtccatttt
    33601 tgcggcagga tgaaagagat cagcaagcct gcgcaggcaa acgcaaaaat gaccgccatg
    33661 accgtttttt tggcccgcca taaaactgcg attagattaa gtaaatcaat ttcattatta
    33721 ctgggcgacg ccagaggata ctcaggaaaa tgagcgtcac ttccctgttt aatattcagt
    33781 gatgacatgg ggtataacct gaaaatgagt ccaatagggg aaatctccac ggcgctcact
    33841 ttaccagttg gtgataatta tccgaagctg aagtttgtaa attccttcca ctgaatgcaa
    33901 cattcactct atttaagaga aatattcatc aaacctataa atcaaacaaa taaaaaaaac
    33961 cataattatt ttctgtaatt atgggtttat agatattatt aatttacctg tttagcgttg
    34021 cgcgaataat cggcccaatt tttacaaacg cccctggaga gataatatcc acatgcgcac
    34081 aatcctgacg atagatatcc agctcggcta tccacggcga ccaggcgcgt tcggggctca
    34141 taccttcctg aagcgtgcgt tcagcaacaa acagcgtcgc tttaccatca aacggtacgc
    34201 tatgagcagt cgtcagcagg cgcacagcat cagcgtagtt gccttcaatg gtggtaaaca
    34261 actccgttga agtacttccc tgctgtgccg ccaggaaggc ctcgcgctcg cggttaatct
    34321 ccgccagcac ttccgggtcc agaccattag cctctttttc ctgccagttt tgcgtttctg
    34381 gcggccaggt atccagcaag ccaagaaatg ccacctgttc gccacgggca cgcagtcgcg
    34441 ccgcaatgcc ctgcgccagc gtaccgccaa gggaataccc cagcaggtaa taagggccgt
    34501 gcggttgttg ctcaagtagc gttgccagat gcgcttcgca gacttcatcc aagtttgtcg
    34561 ctatctgcat ggggccatga gggcgcggtg actgaatgcc gataatcgac cattgtggat
    34621 cgagataacg cgagagcacg ctgaactgcc aggcaaaacc ggacgcagga tggaaacaaa
    34681 acagcgtcgg gccattacct tcacgcaacg gcagaatggt ttcgaatccc atgcgccggg
    34741 cgctgtcttc ttcaccatca ataatcgttg ccagtttggc gacggttgac gcgaccatca
    34801 cctgccccgg cgtcacctga cgagcaaact gtcgacttaa ctgcgctgcc agtttcattg
    34861 ccagtagcga atgaccgcca agcgcaaaga aatcagcatc ggcatcctgc acgtcacaac
    34921 ccagcaacga cgcgaatgcc gcggcgataa tcttttcact gcccgctttc ggtgcacgtc
    34981 ccgacgcttg tgtcttcagt tcaggcaacg gtaaggcttt gcgatccagc ttgccgttgg
    35041 cgctaagagg taactgtgga agttgcagca gaaccaccgg caccatatgc ggcggcaatg
    35101 tttcgcgaag ctgtgcctgt aatgcgctgg tatccaacgg cagacctgat tgcgacacca
    35161 gatagcccac caactgacgc gcatcaccac cggtggctgc cgccaggtta atcacacagg
    35221 cgtgggtaac ggcttgtttg acatccggca gcgcctgcat cacgcgatcg atttcgccca
    35281 gttcgatacg ctgcccgcga atttttagct gatcgtcact gcgcccgagg tactccaccg
    35341 cgccgttatc cagccagcgg gcgacgtctc cggtacgata catccgttca cctggggcaa
    35401 aaggatcggc aataaaacgg ctggcggtca gatcgggtcg tccaagatac ccctgcgcca
    35461 gttggatgcc ggtgagatag agatcacccg ccacacccgg cggcaccgga tgcatcatcg
    35521 catcaagaat acgcagaccc gtattccata ccggataacc aatcggcaca ctgctgccgc
    35581 gcacctgtgc cagttcctcg ccaaaagccg gataccagct gacatctacc gccgcttccg
    35641 tcgggccata caggttatgc aacggcgcgc cggttaactg ttgccattcg cggcataaat
    35701 cagccggtaa ggcctcacca ctacagaaaa cctgtttcaa cgtcgcgcaa ttctggcgag
    35761 cggtttgcgg cgtcagcgat gcaacaaatg ccgccagcat cgacggcaca aagtgcgtgg
    35821 tcgttacgcc atattcggca aagaattgct gcatagcgag cgggtcacgg tgcgcttccg
    35881 gttcagccat caccagtttt gcccctgcga taaacggcca gaaaaactcc cacaccgaga
    35941 catcaaaact gcacggcgtt ttttgggcaa cgacatcttc gcctgtaagc gggtagtgat
    36001 tttgcatcca taacaggcgg ttaacgatag ccgtctgccc gaccattacc cctttcggcc
    36061 tgccggtgga gccagaggta aagatgatat aagccgtgtg gtgcggttgt gaaagttgca
    36121 gcggcgcact gccctggggg gtaagcgggg cgttatagca aaggcttgtt aaattgggaa
    36181 catcgctaaa gcgcggcagt tgatcgtcgg tggtaattaa cagcgacgga cgcgcatctt
    36241 ccagcatcat tttcaggcga tcgtccggat agccggtatc cagcggtagc caggccgcgc
    36301 cagcttcaac tattgcatgg agtgccaggg tcaaaaagac cgagcgcggt agtgccaccg
    36361 ccacgctgtc ccctggttta acgccgcgct cacgcagcag attcgccagc gccaccacct
    36421 gctcgcgcat ttcccgatag ctgaacaggt aacgcgcatc tgccagcgcc ggagcatccg
    36481 gtgtttttgc cgcttgttct gccaccagcg cgctcagcgt ggtttctgga atctcaacct
    36541 gagtggcgtt gatctgcgcc agctgcgcat actcacctgg cagcataata tcgacatcgc
    36601 cgcacaacag cgccggatcc gcggcgaact gggcaatcag cattttcagg cgttcagcat
    36661 gctggattaa cgttggctca tcgtaacgct gtttattggc gaggatctca atactcaaat
    36721 caccgtgttc atccgggaac agggccagtt caaggtcatt aaccggaccg gttgccaggg
    36781 tatgggtttg cgtctgaaca ccaggaatat ccagttggta atcaaatacc ttgatattga
    36841 gtaccggacc gaacagcggt tcctctcctg ccgctcgccc gctgtcacgg acaatttgtt
    36901 cggcatcgta acgttgatga cgacgcattt ttttcagttg tgctgccagt cgggttgcca
    36961 gttccggcag cgtttcttgc gccgcaatgt gaatacccaa cggtaaaacg ttgagcacgg
    37021 gtccgctagc cgtcagcgcc gccgagccca gtcgacgcat aaagataaat ccggcggcat
    37081 agtccatgcg attgcacaat cgccccagcc acaaggctgc cagcgcaagg gctaaatcgg
    37141 tacgctgcac acctgaaagt tgcgtagcca gctggcggaa ttccccgtcg gtaaattcca
    37201 gtttcaggcg cagaatatct gccgaagcgc tgcgccccgc taaaggtgcc ggagaaagtg
    37261 acgcgggcgg cggcagttga cgacgctgtt ctgcccagaa tgccgcatca cgctgccagg
    37321 cttcgctttc gcggtattgc tggtactctt ccactacatc agcgaaaggc gtaaacggcg
    37381 aagcaggcgt tggttcgcca cgcagccatg tgcagtaaat attggcgatc tggcgagtaa
    37441 tggccgggaa actgaagcca tcgaccagca aatggtgata acgctgatac cagtaccagc
    37501 ggttatccgc aacatgtatc agctgatgaa agaccagtgg tttaccgcta tcgacgcgca
    37561 aatcttgttg caaatccgcc agcattaatg cctgcgcagt accgtgcgga tcaatattgg
    37621 ttcgcaggtc gataatttct ggcagttcga atatcagcgc atcatcgacc cactgccaga
    37681 cttcgccgtt atcttccgta aaacgcatcc gcaacgtatc tgcttgcgct agtccgacaa
    37741 ccaccgcgcg ggccagtaat ggcgcatcaa catctccagt taactcaacg taatgcgcca
    37801 cgctccaggc ggagggtaaa tccgacagtt tttctgccat ccagatgccg ggctgtgcgg
    37861 cgaccaaagg taaatgctgg ctcattgtgc ctcctgcaac tgggtgaaat tggccggtgt
    37921 cagagtacgc cagtggactt ccagccactg ctggcaggac gcctgtgact gcggctgaca
    37981 cacaatgtcc cagcctgccg gtaaggcgca ttgttgcggc cacagactga attgcccctg
    38041 cgcattgcgc aaaatgtaaa acgctccctg tggatcatcg aagggattac tgaatgccat
    38101 attcaactcc tgtcatggaa aagtggttgc cagaggtcga ttagcccctg catcaagcca
    38161 ccgcgccaac aaagcgcatc atgtccgccg tcaacctgac gccagaaaat ggattctttt
    38221 atggggtgta attgcgcata cagcgcctga ttggcccgca taatcatcgg ctcgcgaata
    38281 cccgcttcca gcacaatgcg cagaccttcg gcgctaactt caccagcttt aagcttttca
    38341 agtaacacgc cctcttgctg cccgccccga tgcggccacc agtacgatcc tgactggctt
    38401 aatacacagc caaagcgttc aggccagtgc agtccggcat acagcgcgga cagcccacca
    38461 aaactctgcc cggcgaccac ggtgcgatcg gcgcgatcgc taaaaggggc aatagctttc
    38521 accaggggta ataactcttg ctgtactgcc agccagaaat ccgcattaca cggcagttcg
    38581 tgggcgcggt gcgtggtgtc gatagcgtcg atcaacacat acacggcggg aggaagttgc
    38641 tgacgatggg tcagcgaagt cagcactggc cagacgggca tactttgcgc ccaaaattcg
    38701 ccatcgagca aaactgccag cgggcgttct tctgctgttg catcgccggt ggtaaaaatc
    38761 catacacgcc gtgacttttt caaccgttca cttttccaga taatttcttt ggcaggtatt
    38821 tctggcgctt gcggacaatc ccatcccggt tgcagaggcg cttgcggcat ttcgagtgca
    38881 gaaacagcgt gccctcgccc gcctttccag ctttgtaggt tcagcggatc ggctatcgcc
    38941 tggggtaata gttttcgcca gccttcgcgc aattcgaggc gatcggggga tggtacagaa
    39001 aaaatgtcat cgcgttcggt gggaataaag cagtagctgc cgcgccagtt ggcattgagt
    39061 tgtgtcttcc actgccagac gttagtgcct gcaattcgct gcatcgactg gggctggctg
    39121 ttctgatggt gatcggtcac accagtgatg tagacccata cgcgctttat cgtcgagtat
    39181 tcttcagaac cttggggatc acgccaccag aaagtgacct caaacatttc gtcattcaga
    39241 cgctgccatt ccgggccatg tttcgactgc caccagctct cacttcctac ttttaacgcc
    39301 gtcacaccat aaccccatgt ttactgtgca atttttcatt gattgcagaa atatattgat
    39361 aatattattg ataactattt gcatttgcaa tagcgtaatg gcgcgccgtg ggaagcgcgg
    39421 acattaatta accaactgca ctgcgtgtct ttcaggatca aaggttttcg cggtagcggg
    39481 atgcgtcgtg ttgatgacga ccatgcccga cagttgcaat tcgtggcaaa aatgcaggaa
    39541 taaaacaatg aacaagaaga ttcattccct ggccttgttg gtcaatctgg ggatttatgg
    39601 ggtagcgcag gcacaagagc cgaccgatac tcctgtttca catgacgata ctattgtcgt
    39661 taccgccgcc gagcagaact tacaggcgcc tggcgtttcg accatcaccg cagatgaaat
    39721 ccgcaaaaac cccgttgccc gcgatgtgtc ggagatcatc cgtaccatgc caggcgttaa
    39781 cctgaccggt aactccacca gtggtcagcg tgggaataac cgacagattg atattcgcgg
    39841 tatgggtccg gaaaacacgc tgattttgat tgacggcaaa ccggtaagca gccgtaactc
    39901 ggtgcgtcag ggctggcgtg gcgagcgcga tacccgtggt gatacctcct gggtgccacc
    39961 tgaaatgatt gaacgtattg aagttctgcg tggtccggca gctgcgcgtt acggcaacgg
    40021 cgcggcgggc ggcgtggtta acataattac caaaaaaggc agcggtgagt gacacggctc
    40081 ctgggacgct tatttcaatg cgccagaaca taaagaggaa ggtgccacta aacgcaccaa
    40141 cttcagcctg accggtccgc tgggcgacga attcagcttc cgcttgtatg gcaacctcga
    40201 caaaacccag gctgacgcgt gggatatcaa ccagggccat cagtccgcgc gtgccggaac
    40261 gtatgccacg acgttaccag ccgggcgcga aggggtgatc aataaagata ttaacggcgt
    40321 ggtgcgctgg gacttcgcac cattgcaatc gctggaactg gaagcgggtt acagccgcca
    40381 gggtaacctg tatgcgggcg atacccagaa caccaactct gacgcttaca ctcgctcgaa
    40441 atatggcgat gaaaccaacc gcctgtatcg ccagaactac tcgctgacct ggaacggtgg
    40501 ctgggataac ggcgtgacca ccagcaactg ggtgcagtac gaacacaccc gtaactcgcg
    40561 tattccggaa ggtctggcgg gcggtaccga agggaaattt aacgaaaaag cgacacagga
    40621 tttcgtcgat atcgatcttg atgacgtgat gctgcacagc gaagttaacc tgccgattga
    40681 tttcctcgtt aaccagacgc tgacgctggg tacggagtgg aatcagcaac ggatgaagga
    40741 cttgagttcc aatacccagg cgctgaccgg ggcgaacacc ggcggcgcta ttgatggtgt
    40801 gagtgccacc gaccgtagcc cgtattcaaa agcagaaatt ttctcgctgt ttgccgaaaa
    40861 caacatggag ctgactgaca gcaccatcgt aacgccgggg ctgcgtttcg atcatcacag
    40921 tattgtcggc aataactgga gcccggcgct gaacatatcg caaggtttag gcgatgactt
    40981 cacgctgaaa atgggcattg cccgtgctta taaagcaccg agcctgtacc agactaaccc
    41041 gaactacatt ctctacagta aaggtcaggg ttgctatgcc agcgcgggcg gctgctatct
    41101 gcaaggtaac gatgacctga aagcagaaac cagcatcaac aaagagattg gtctggagtt
    41161 caaacgcgac ggctggctgg cgggcgtcac ctggttccgt aacgattatc gcaataagat
    41221 tgaagcgggc tatgtggctg taggacaaaa cgcagtcggc accgatctct atcagtggga
    41281 taacgtgccg aaagcagtgg ttgaaggtct ggaaggatcg ttaaacgtac cggttagcga
    41341 aacggtgatg tggaccaata acatcactta tatgctgaag agtgaaaaca aaaccacggg
    41401 cgaccgtttg tcgatcatcc cggagtatac gttgaactca acgctgagct ggcaggcacg
    41461 agaagatttg tcgatgcaaa cgaccttcac ctggtacggt aagcagcagc cgaagaagta
    41521 caactataaa ggtcagccag cggttggccc ggaaaccaaa gaaatcagtc cttacagcat
    41581 tgtgggtctg agcgcgacct gggatgtgac gaagaatgtc agtctgaccg gcggcgtgga
    41641 caacctgttc gacaaacgtt tgtggcgtgc gggtaatgcc cagaccacgg gcgatctggc
    41701 aggggccaac tatatcgcgg gtgccggggc gtatacctat aacgagccgg gacgtacgtg
    41761 gtacatgagc gtaaacactc acttctgatg ctaacgtcag attgttgaca aagtgcgcgt
    41821 cgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaaaatctt gccaattcaa
    41881 tatattgcag gacc
//
LOCUS       DAZGSP010000040        41650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZGSP010000040 DAZGSP010000000
VERSION     DAZGSP010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.040"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(236..1828)
                     /gene="lsrK"
                     /locus_tag="ACHK74_002245"
     CDS             complement(236..1828)
                     /gene="lsrK"
                     /locus_tag="ACHK74_002245"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFP0221061.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1907..2860)
                     /gene="lsrR"
                     /locus_tag="ACHK74_002246"
     CDS             complement(1907..2860)
                     /gene="lsrR"
                     /locus_tag="ACHK74_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFP0221062.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3109..4644
                     /gene="lsrA"
                     /locus_tag="ACHK74_002247"
     CDS             3109..4644
                     /gene="lsrA"
                     /locus_tag="ACHK74_002247"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFP0221063.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYVRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMTDRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4638..5666
                     /gene="lsrC"
                     /locus_tag="ACHK74_002248"
     CDS             4638..5666
                     /gene="lsrC"
                     /locus_tag="ACHK74_002248"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFP0221064.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5666..6658
                     /gene="lsrD"
                     /locus_tag="ACHK74_002249"
     CDS             5666..6658
                     /gene="lsrD"
                     /locus_tag="ACHK74_002249"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFP0221065.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6670..7692
                     /gene="lsrB"
                     /locus_tag="ACHK74_002250"
     CDS             6670..7692
                     /gene="lsrB"
                     /locus_tag="ACHK74_002250"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFP0221066.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7719..8594
                     /gene="lsrF"
                     /locus_tag="ACHK74_002251"
     CDS             7719..8594
                     /gene="lsrF"
                     /locus_tag="ACHK74_002251"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFP0221067.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGNTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8618..8908
                     /gene="lsrG"
                     /locus_tag="ACHK74_002252"
     CDS             8618..8908
                     /gene="lsrG"
                     /locus_tag="ACHK74_002252"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFP0221068.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8965..9723
                     /gene="tam"
                     /locus_tag="ACHK74_002253"
     CDS             8965..9723
                     /gene="tam"
                     /locus_tag="ACHK74_002253"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFP0221069.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIKYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(9727..10641)
                     /locus_tag="ACHK74_002254"
     CDS             complement(9727..10641)
                     /locus_tag="ACHK74_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFP0221070.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10849..12300)
                     /gene="uxaB"
                     /locus_tag="ACHK74_002255"
     CDS             complement(10849..12300)
                     /gene="uxaB"
                     /locus_tag="ACHK74_002255"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFP0221071.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLCTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12527..13945)
                     /locus_tag="ACHK74_002256"
     CDS             complement(12527..13945)
                     /locus_tag="ACHK74_002256"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP0221072.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFTIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14084..14443)
                     /locus_tag="ACHK74_002257"
     CDS             complement(14084..14443)
                     /locus_tag="ACHK74_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFP0221073.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14443..15369)
                     /gene="glsB"
                     /locus_tag="ACHK74_002258"
     CDS             complement(14443..15369)
                     /gene="glsB"
                     /locus_tag="ACHK74_002258"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFP0221074.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15433..16820)
                     /gene="sad"
                     /locus_tag="ACHK74_002259"
                     /pseudo
     CDS             complement(15433..16820)
                     /gene="sad"
                     /locus_tag="ACHK74_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
     gene            16921..17802
                     /locus_tag="ACHK74_002260"
     CDS             16921..17802
                     /locus_tag="ACHK74_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0221075.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17880..18994
                     /gene="yneK"
                     /locus_tag="ACHK74_002261"
                     /pseudo
     CDS             17880..18994
                     /gene="yneK"
                     /locus_tag="ACHK74_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            19144..20334
                     /gene="ydeA"
                     /locus_tag="ACHK74_002262"
     CDS             19144..20334
                     /gene="ydeA"
                     /locus_tag="ACHK74_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFP0221076.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(20359..21024)
                     /gene="marC"
                     /locus_tag="ACHK74_002263"
     CDS             complement(20359..21024)
                     /gene="marC"
                     /locus_tag="ACHK74_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFP0221077.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            21236..21670
                     /gene="marR"
                     /locus_tag="ACHK74_002264"
     CDS             21236..21670
                     /gene="marR"
                     /locus_tag="ACHK74_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFP0221078.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            21690..22073
                     /gene="marA"
                     /locus_tag="ACHK74_002265"
     CDS             21690..22073
                     /gene="marA"
                     /locus_tag="ACHK74_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFP0221079.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            22105..22323
                     /gene="marB"
                     /locus_tag="ACHK74_002266"
     CDS             22105..22323
                     /gene="marB"
                     /locus_tag="ACHK74_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFP0221080.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(22380..23819)
                     /locus_tag="ACHK74_002267"
     CDS             complement(22380..23819)
                     /locus_tag="ACHK74_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP0221081.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(23844..25517)
                     /locus_tag="ACHK74_002268"
     CDS             complement(23844..25517)
                     /locus_tag="ACHK74_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFP0221082.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     SAAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYDDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(25573..25884)
                     /locus_tag="ACHK74_002269"
     CDS             complement(25573..25884)
                     /locus_tag="ACHK74_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP0221083.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(25912..27234)
                     /locus_tag="ACHK74_002270"
     CDS             complement(25912..27234)
                     /locus_tag="ACHK74_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP0221084.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLALISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEFRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYVVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            complement(27349..27660)
                     /locus_tag="ACHK74_002271"
     CDS             complement(27349..27660)
                     /locus_tag="ACHK74_002271"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP0221085.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            27859..28557
                     /locus_tag="ACHK74_002272"
     CDS             27859..28557
                     /locus_tag="ACHK74_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP0221086.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(28602..29501)
                     /gene="eamA"
                     /locus_tag="ACHK74_002273"
     CDS             complement(28602..29501)
                     /gene="eamA"
                     /locus_tag="ACHK74_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFP0221087.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            29696..30883
                     /gene="ydeE"
                     /locus_tag="ACHK74_002274"
     CDS             29696..30883
                     /gene="ydeE"
                     /locus_tag="ACHK74_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFP0221088.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLSDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            31010..31105
                     /gene="mgtS"
                     /locus_tag="ACHK74_002275"
     CDS             31010..31105
                     /gene="mgtS"
                     /locus_tag="ACHK74_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFP0221089.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            31105..31209
                     /gene="mgtT"
                     /locus_tag="ACHK74_002276"
     CDS             31105..31209
                     /gene="mgtT"
                     /locus_tag="ACHK74_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFP0221090.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(31324..32214)
                     /gene="dgcZ"
                     /locus_tag="ACHK74_002277"
     CDS             complement(31324..32214)
                     /gene="dgcZ"
                     /locus_tag="ACHK74_002277"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFP0221091.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTSALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(32469..32861)
                     /gene="ydeI"
                     /locus_tag="ACHK74_002278"
     CDS             complement(32469..32861)
                     /gene="ydeI"
                     /locus_tag="ACHK74_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFP0221092.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            33137..33655
                     /locus_tag="ACHK74_002279"
     CDS             33137..33655
                     /locus_tag="ACHK74_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFP0221093.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALVQLLQLLL"
     gene            complement(33700..35745)
                     /gene="dcp"
                     /locus_tag="ACHK74_002280"
     CDS             complement(33700..35745)
                     /gene="dcp"
                     /locus_tag="ACHK74_002280"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFP0221094.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            35882..36628
                     /gene="ydfG"
                     /locus_tag="ACHK74_002281"
     CDS             35882..36628
                     /gene="ydfG"
                     /locus_tag="ACHK74_002281"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFP0221095.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            36717..37403
                     /gene="rspR"
                     /locus_tag="ACHK74_002282"
                     /gene_synonym="ydfH"
     CDS             36717..37403
                     /gene="rspR"
                     /locus_tag="ACHK74_002282"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFP0221096.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            37581..37784
                     /gene="ydfZ"
                     /locus_tag="ACHK74_002283"
     CDS             37581..37784
                     /gene="ydfZ"
                     /locus_tag="ACHK74_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFP0221097.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(37820..39280)
                     /locus_tag="ACHK74_002284"
     CDS             complement(37820..39280)
                     /locus_tag="ACHK74_002284"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP0221098.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDEKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(39369..40652)
                     /gene="ydfJ"
                     /locus_tag="ACHK74_002285"
                     /pseudo
     CDS             complement(39369..40652)
                     /gene="ydfJ"
                     /locus_tag="ACHK74_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            41256..41369
                     /locus_tag="ACHK74_002286"
     CDS             41256..41369
                     /locus_tag="ACHK74_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFP0221099.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            41438..>41650
                     /gene="ydfK"
                     /locus_tag="ACHK74_002287"
     CDS             41438..>41650
                     /gene="ydfK"
                     /locus_tag="ACHK74_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFP0221100.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNQ"
BASE COUNT        11016 a        10321 c         9563 g        10750 t
ORIGIN      
        1 agtttattaa accccttttg catccattag tgtctttagg aatattcgct ataaaataag
       61 ggttgtgctt aatgctttaa gaaaaatagc aattttccct tgaatatagt actggtgatg
      121 gaacgatgaa tctgcaagct ggcttttaac aagccagctc taaaagaagg gaaataagaa
      181 taactatact caaaaactaa cagccacggt catcatgatg tggctgtcaa tgaaactata
      241 acccaggcgc tttccataac gacgtcgtca gtccatgatc aaccagcccc agctgatcct
      301 gataaactgc ctgccactta tcgcgtgaat cctgataaag ttcatgcttt tccgggtctg
      361 gtgtgtgcgt ccgttcccag cgaaccaggc gttctccggt ttctgccatt gatgaaaaaa
      421 ttccggcacc gacgccagct gcaatggcac atcctaatgc agtggcttct ttgaccaccg
      481 gaatattgac gggtaatccc gagacatcag cgagaatttg actccataat ttcccttttg
      541 aacctccgcc tgcaaagact aacgatgaag gatgaatatt cgagaaatca gcaatttgct
      601 gcaagttaca cgctgataca atcgccgcat tttcttccag cgcacggaac aatgtcgctt
      661 tgttacattt atccgggtca atggacaagt taataaagga aggcgcagcg tgataccagg
      721 ttttaaagcg cattctgtcg gagaagatcg gcattacgcc ccacgaccca ggcggcaccc
      781 gactggccat ctcttccagc agcgtatagg tgtcgatgcc taaacgttcg gcaatcagtt
      841 tttcttcggc acagaaagca tcgcggaacc agcgcatggt gagtccggta aaaaagctta
      901 tagattcagc ttgtaccatg ccaggaataa catgaggatt aacgcgcacg ttcatttctg
      961 ggtctgtcac cggcgcggct aaatttacaa cttgctgcca gaatgtgccg ccaagaaccg
     1021 cggtttgtgc cggacgcaca acgcctaacc caaggcaacc aagctgcacg tcgcctcctc
     1081 caacgaccac cggagtgccc gccttcagac cgcagagttc cgccgcttgt gaacttacca
     1141 cgcccagcaa tgtgccggtt tctttgacag gagaaagaat atcggcacgt aggccagcca
     1201 tatccagcaa tgcaggtttc cagtcacggg tggttagatc aagaagtccc gtggtgccag
     1261 cgttagaggg atccaccgcc agttcgccgc tgagcatata ggccagccag tcgctgatca
     1321 tggtgatggt tgatgcctga cggtaaatat cggaacgatg gtgcgccagc caaagtaatc
     1381 tggggatggc acttaaagcc agtgtttgtc cggtcgcgcg ataaacttcg ttttcaaagg
     1441 tattgttgtg cagttcttta agttcgctaa cttcgcgtgc cgctctggca tccacattgg
     1501 cgcaggccca gatcggggct ccttcattat tatataaaac aatgccttca cgcatcgaac
     1561 atgccgaaac ggcagcgata tactccgggg ctatgccggc gttgtgcagc gcctggcgca
     1621 tacactcaca cgccagttgc cagtttttgt tgagatcaaa ttccatagaa ccaggaacgt
     1681 ccggtactgc caggtgccgc cactccgcct gtcccactgc tatttgattg ccttccaggt
     1741 cgaatatcac agcccgaata cttccggtac ctgcatccag cgccatcagg taatactttg
     1801 attctgaagg ggtaaagagt cgagccatta gattatcctc ggcttatgcg ttctgtctgt
     1861 tcctctatac gttctccatc attcccggta ataaggtcat gcaaatttaa ctacgtaaaa
     1921 tcgccgctgc tgtgtcctga tcggtaacca gtgcgttgat ataaccgcct ttcattgcag
     1981 cggcaattgc ttcggcttta ttttctcccc ctgccacgcc aacccggacg ggtatggtct
     2041 tcagcgagct taaaggtaag ccaatcagtt cgttatgtat tttaatatcc gtgacaacgt
     2101 caccttttgc atcaaaaaag tagcctaaaa tgtcgccaac cgcccctttt cgaccaatca
     2161 ttaactgttc gccctggctg atataaccgg agcgaatgat cgtcgcgtcg tcctgttgac
     2221 tgacggcccc aatgccgaca atcgccacat ccgctgcttg cgcggctaac agaacatctt
     2281 tgacgcaatt ttcatttttt agcgtacggg caatgtcagc ggaggatgcc cgcaacggag
     2341 ccggaataat attcacactg cacgccgcgt taagctgccc gatacccgtc atataagaac
     2401 cgacgccacc ggagagcgtg accaggcgaa tttgctgtga cgaaataaaa ccacttaagc
     2461 gttgcagcgt attcatagtt gcctcgccaa aaccaatcgc cagcatctgt tgtggttgaa
     2521 gtaaactcat caacatatgc gccgcgccta tccccagtcg cccgccgaca tcagcatccg
     2581 caagcccagg gatcacccgg acatgttgca gcgaaaactg acgacgtaat tgtgtttcat
     2641 attccagaca gccttcaaag cgagaattaa tctgcacgcg aataatgccg gactgatgcc
     2701 ctttctccag taatcgcgac actttcaaac gggtcaggcc gagacgatcg ctgatctcac
     2761 tctgggtcaa cccgtcgtgg tagtaaaacc acgcgatccg cgcgacctgt tcttcttcac
     2821 acattccctg ttctgaaatt accgaatcgt tgattgtcat aattcattct tcactttgaa
     2881 catatttaaa tctttaatgc aattgttcag ttcttgcgca tttatatctg tgatggcaac
     2941 cacagtttga ctctacgagc atgaacaaac gcaaccgtga aaatcaaaat agcataaatt
     3001 gtgatctatt cgtcggaaat atgtgcaatg tccacctaag gttatgaaca aattaaaagc
     3061 agaaatacat ttgttcaaaa ctcacctgca aaactgaacg ggggaaatat gcaaacgagt
     3121 gatacccgcg cgttaccgct actttgcgcc cgctcggttt ataaacagta ttcaggggtc
     3181 aatgtcctga aaggcatcga ttttacgttg catcaggggg aggtccacgc cctgctcggc
     3241 ggcaatggtg ccggtaaatc gacgttaatg aagattattg ccggtattac ccctgctgat
     3301 agcggtacgc tggagattgg gggcaacaac tacgtcagat taacgccagt tcatgctcat
     3361 cagctgggta tttatctcgt tccccaggaa ccgctgcttt tcccaagcct gtcgataaaa
     3421 gaaaacatcc tgtttgggct ggcaaaaaaa cagctctcca tgcagaaaat gaagaacttg
     3481 ctggcggcgc tgggctgcca gtttgatctg catagtctgg caggatcgct ggatgtcgcc
     3541 gatcgccaaa tggtggaaat cctccgcggg ctgatgcgcg actcgcggat tctgatcctc
     3601 gatgaaccta ccgcctcgct tacccctgcg gaaaccgaac gcttgtttag tcgcttgcaa
     3661 gagctgcttg ctactggcgt gggtattgtt tttatctcgc ataagctgcc ggaaattcgc
     3721 cagattgccg atcgaattag cgtgatgcgc gacggaacca tcgccttaag cggcaaaacc
     3781 agcgaactgt ctaccgacga cattattcag gccattacgc cagcggtacg ggaaaaatcg
     3841 ctctctgcca gccaaaaatt atggctggag ttacctggta accgcccaca acatgctgcc
     3901 ggaacgccgg tgctgacact ggaaaatctg accggcgaag gtttcaggaa tgtcagcctg
     3961 acgctcaatg ccggagaaat tctgggcctg gctgggctgg tgggagccgg acgcacagaa
     4021 ctggccgaga cgctctatgg tctgcgtact ttgcgtggcg gacgcattat gctgaatggt
     4081 aaagagatca ataaattatc cactggagaa cgtttactgc gcggtctggt ttatctgccg
     4141 gaagatcgcc agtcatccgg actgaatctc gatgcttcgc tggcctggaa cgtctgcgcc
     4201 cttactcata accttcgtgg attctgggcg aaaaccgcga aagataatgc caccctggaa
     4261 cgttatcgtc gggcgctgaa tattaaattt aaccaaccgg aacaagctgc acggacatta
     4321 tccggtggca accagcaaaa aatcctcatt gccaaatgcc tggaagcttc gccgcaagta
     4381 ttgattgtcg atgagccgac gcgcggcgtg gatgtctcgg cacgtaatga tatctaccag
     4441 ctgttgcgca gcatcgccgc acagaatgtg gctgtgctgc ttatctcctc tgatctggaa
     4501 gagatcgaac tgatgacaga tcgtgtgtat gtgatgcatc agggcgaaat tacccactct
     4561 gcactgaccg ggcgcgatat taatgtcgag actattatgc gcgttgcctt cggcgatagt
     4621 cagcgtcagg aggcatcatg ctgaagttta ttcagaacaa ccgtgaaatc acggcattgc
     4681 tggcggtggt gctgctgttt gtattacccg gttttctcga ccgccagtat ttaagtgtgc
     4741 aaacgctgac catggtttat agcagcgcgc aaatcctgat cctgctggca atgggcgcga
     4801 cgctggtaat gcttacgcgc aatattgatg tttcagtggg ttcgattacc ggaatgtgcg
     4861 cggtgctgtt ggggatgtta ctgaacgcag gatattcact acctgttgcc tgtgtcgcga
     4921 ctttactgct tggtttgctc gcgggatttt tcaacggtgt cctggtcgcg tggctaaaga
     4981 tccctgccat tgttgccacc cttggcacgt tagggttgta cagaggcatc atgttgctgt
     5041 ggactggcgg taaatggatt gaagggttac ccgccgaact gaaacagctc tccgcaccgc
     5101 tgctgtttgg cgtttccgcg attggttggt tgacgataat tctggtggca tttatggcct
     5161 ggctgctggc aaagacggcg tttggacgca gtttttatgc cacgggcgat aatttacagg
     5221 gcgctcgtca actgggtgtt cgtacggaag ccattcgcat tgtggcattt tcgttgaacg
     5281 gctgcatggc ggcactggcg ggaattgtgt ttgcttcgca gattggtttt atccccaacc
     5341 agaccggtac cgggctggag atgaaagcaa ttgcagcctg cgtgctgggc ggcattagtt
     5401 tgctcggcgg ttccggtgcg atcatcggtg cggtactcgg cgcatggttc ctgacgcaga
     5461 tcgatagcgt actggtgctg ttgcgcattc cggcatggtg gaacgatttt atcgcgggcc
     5521 tcgtgctgct ggcggtgctg gtgtttgatg gacgcctgcg ttgtgcgctg gaacgtaatc
     5581 tacggcggca aaaatatgcc cgctttatga cgccaccgcc atccgttaaa cccgcttcgt
     5641 caggtaaaaa acgggaggcc gcataatgcg tattcgctac ggttgggaac tggctcttgc
     5701 cgcactgctc gttattgaga ttgtcgcatt tggtgcaatt aacccgcgaa tgttagatct
     5761 caatatgttg ctgttcagca ccagtgactt tatctgcatt ggcattgtcg ccctaccgct
     5821 gacgatggtg attgtcagtg gcgggatcga tatttcgttt ggttcgacca tcggcctctg
     5881 cgccattgca ttgggcgtac tgtttcaaag tggtgtgccg atgccgctgg cgatactcct
     5941 gaccttactg ctcggcgcat tgtgcgggct gatcaacgcc ggattaatta tctataccaa
     6001 agttaacccg ctggtgatta cgcttggcac gctgtatctg tttgccggaa gcgctctgct
     6061 gctttccggt atggccggag cgacggggta cgaaggtatt ggtggattcc cgatggcgtt
     6121 tacagatttc gctaacctgg atgtgctggg actccccgtt ccgctgatta tcttcctgat
     6181 atgtctcctc gttttctggc tctggctgca taaaacccat gccggacgta atgtgttttt
     6241 gattgggcaa agcccgcgcg tggcgcttta tagcgcgatt ccagttaacc gcaccttatg
     6301 tgcgctctat gccatgacgg ggctggcgtc tgcggtcgcc gctgtgctgc tggtatcgta
     6361 ttttggttca gcacgttccg atctcggtgc gtcgtttctg atgcccgcca tcaccgccgt
     6421 ggtgcttggc ggtgccaata tttatggtgg ttccggttcc attatcggca ccgccattgc
     6481 ggttttatta gtgggatatt tgcaacaagg tttgcaaatg gcaggagtgc caaatcaggt
     6541 gtccagcgcc ctttccggtg cgctacttat cgtcgttgtc gtaggtcgtt ccgttagcct
     6601 gcatcgccag caaattaaag agtggctggc gcgtcgggcc aataacccat tgccataaag
     6661 gatatcttca tgacacttca tcgctttaag aaaatcgcct tacttagcgc tcttggcatt
     6721 gccgcaatct ctatgaatgt gcaggccgca gagcgtattg catttattcc caaactggtt
     6781 ggcgtgggat tttttaccag cggtggcaac ggcgcacaac aagcgggtaa agagctgagc
     6841 gttgatgtga cctacgacgg gccgacagaa cccagtgttt ctggtcaggt acagttgatt
     6901 aataacttcg tcaatcaagg ttataacgcc attatcgttt ctgcggtttc gcctgatggc
     6961 ttgtgtccgg cactgaaacg cgccatgcaa cgtggtgtga gagtgctgac ctgggactct
     7021 gatactaaac cggagtgccg ctcttactac attaatcagg gaacgcccgc ccagttggga
     7081 ggtatgttgg tggatatggc ggcgcgtcag gtgaataaag acaaagccaa agtcgcgttt
     7141 ttctactcaa gccccaccgt tacggaccaa aaccagtggg tgaaagaagc gaaagcgaaa
     7201 atcgccaaag agcatcctgg ctgggaaatt gtcactacgc agtttggcta taacgatgcc
     7261 actaaatcat tacaaaccgc agaaggaata ttaaaagcgt atagcgatct cgacgccatt
     7321 atcgcccccg acgccaacgc cctgcccgct gccgcacaag ccgcagaaaa cctgaaaaat
     7381 gacaaagttg cgattgtcgg attcagtacg ccaaacgtga tgcgtccata tgtggaacgc
     7441 ggcacggtga aagaatttgg cctgtgggat gtggttcagc aaggcaaaat ttcagtgtat
     7501 gtcgcggatg cattattgaa aaaaggatca atgaaaacgg gcgacaagct ggatatccag
     7561 ggcgtaggtc aggttgaagt ctcgccaaac agcgttcagg gctatgacta cgaagcggat
     7621 ggtaatggca tcgtactgtt accggagcgc gtgatattca acaaagagaa tatcggcaaa
     7681 tacgatttct gatgtgcatt acttaaccgg agtaagttat ggcagattta gacgatatta
     7741 aagatggtaa agattttcgt accgatcaac cgcaacaaaa tatccctttt accctgaaag
     7801 gttgcggtgc gctggattgg ggaatgcagt cacgcttatc gcggatattt aatccgaaaa
     7861 cgggtaatac cgtgatgctg gcctttgatc atggttattt tcaggggccg actaccggac
     7921 ttgaacgcat tgatataaat atcgccccgc tgtttgaaca tgccgatgta ttaatgtgta
     7981 cgcgcggcat tttgcgcagc gtagttcccc cagcgaccaa taagccggtg gtactgcggg
     8041 cgtcaggtgc gaactctatt ctggcggaat taagtaatga agccgtggcg ttatcgatgg
     8101 atgatgccgt gcgcctgaac agttgcgcgg tggcggcgca ggtttatatc ggcagcgaat
     8161 atgaacatca gtcgatcaaa aatattattc agctggttga tgccggaatg aaagtgggaa
     8221 tgccgaccat ggccgtgact ggcgtgggca aagatatggt gcgcgatcag cgttatttct
     8281 cgctcgcgac tcgaatcgcc gctgaaatgg gggcgcaaat tatcaaaacc tattatgtcg
     8341 aaaaaggttt tgaacggatt gttgccggat gtccggtacc cattgttatt gctggcggta
     8401 aaaaattacc ggagcgcgag gcgctggaaa tgtgctggca ggctatcgat cagggcgctt
     8461 ctggtgtgga tatggggcgt aatattttcc agtctgacca tccggtggcg atgatgaaag
     8521 ccgtacaggc ggtggttcac cataacgaaa cggctgatcg ggcatatgaa ctctatctga
     8581 gtgaaaaaca gtaactgcgg atttaaggag aagaattatg cacgtcacac tggttgaaat
     8641 taacgttcat gaagacaaga ttgacgagtt tatcgaagtt tttcgccaga accacctggg
     8701 ctctgtacag gaagaaggca atttgcgctt cgatgtctta caggacccag aagtgaattc
     8761 gcgcttttat atttacgaag cctataaaga tgaagacgca gtggcattcc ataaaaccac
     8821 gcctcactac aaaacctgtg tcgcgaaact ggaatcttta atgaccgggc cgcgtaaaaa
     8881 acgtctgttc aatgggttga tgccgtgagg cgaatttatc atttttatct acaattgggg
     8941 taacgcgctg acgggagtaa aaatatgtct gactggaacc cctctttata tctgcacttt
     9001 gccgctgaac gatcgcgtcc ggcggtggag ctgcttgcca gagtgccgct ggagaatatc
     9061 aaatacgttg ccgatcttgg ctgtggccca ggtaacagca ccgccctgct gcatcaacgt
     9121 tggcctgcgg ccaggataac aggcatcgac tcgtctccgg cgatgattgc tgaagcgcgc
     9181 agtgctttgc cagactgcca gttcgtggaa gcagacatcc gcaactggca accggaacag
     9241 gcactcgatc tgatttttgc taatgcctca ctgcaatggc tgcccgacca ctacgaattg
     9301 tttcctcatc tggtttcttt acttaatccg cagggcgtgc tggcagtaca gatgccagat
     9361 aactggctgg agccgaccca tgtgctcatg cgcgaagttg cctgggaaca aaactacccg
     9421 gatcgcgggc gtgagccgtt ggctggcgtt catgcttact acgatatttt gagcgaagcc
     9481 ggatgtgagg tcgatatctg gcgaaccacc tactatcacc agatgccgtc acaccaggcg
     9541 attatcgatt gggtgactgc cactggatta cgtccgtggt tacaggatct gaccgagagc
     9601 gaacagcagc tttttcttaa gcgctaccat cagatgctgg aagagcagta tccactgcaa
     9661 gagaacggac agatactgct ggcatttccg cgtcttttta ttgttgcccg gcgtatggag
     9721 taaatatcac gtcagctggt aatgacgatc gggaagaatt tttgctggaa tttcggcttc
     9781 atcgttcatc tgtaacaggt caatttcaat agcgttgcag atggcatcca gtggtaaatc
     9841 attgttttca gtaccgaacg gatcttccag ttcttccgcc agacaatcca gcgaaataaa
     9901 agtgtaggaa atcagcacag agataaaagg cgtcatgtaa tgcaggtcca cgaccagcgc
     9961 gaacggcagc atgatacaaa acagataaac ggtacgatgc aaaatcaggg tgtaggcaaa
    10021 gggaattggc gtataggcaa tgcgctcgca tcctgccagg accgctgaaa tatcattaag
    10081 ccgatcgttg aggctaataa acaggatatc tgaaagctgc ccattgcggc gctgaaccgc
    10141 caaccactcc cccattatta acaagatacg gttagctgga gagttcgaag ccagtacagg
    10201 ctgaagatct tcagtcttga gataatgagc cagcacttcc gcctgtggct gtttgcgtaa
    10261 tgtcatgcgt aaacagtggg cgaaggcgat ttgcaaccgg gcaaactccc ttacacttgc
    10321 cgaatccggc aatgtcgttt ttacctcgcg cagtaacgac cgtgaggcaa tcatcaactg
    10381 cccccaaagt tttcgcgctt caacgtaacg ggcgtacccg gcattattac gaaaaccaag
    10441 aaaaatggcg atggcgacac cgagaatgct gaacggtgcg agggtgaatt tgatgcccag
    10501 atgcgtgtac cagggcagca tgaaaataac agcgatagaa aagagaaaat tgagtagtaa
    10561 gcgcgatgat atcttggata atactgagcc gtgccagaca aaaatacggc gcagccagtg
    10621 ttgttgtgga cgaacaatca tggttatctt caggcgtgaa aaagtcgccc tattaaacgt
    10681 gattacgatc acattctcaa gacgctactt acaaattacc tacaagctta acaacattaa
    10741 caggcgctgc atgtggcatc tgttaatgat aaaggcacat aggatgttgt aactaatata
    10801 gtgaaataag aaacccggtc gaaaccgggt tcagaagtaa cggtgttatt agcacaacgg
    10861 acgtacagct tcgcgcatcc ctttttcaag aatcgcatcc aggtcattag caacctgctc
    10921 gaccagacca ggcacttgcg tcaggtcctg ctcccagtgg tctttttctg ccaacacaat
    10981 cgctaccagt tcctgggtac cgataacacg gtcacgatgc tggctccaga gttgctgata
    11041 acgttccagc cagtgtgcat cgtcctgtac cggatatgtt tctccgttac gctcaccacg
    11101 atagaaggca atcaatgccg ctaatgcgaa agtaaggcgc gccggaagtg tgccttttgc
    11161 cttctgccct gccagcagct gcggcaggat gcgagtgcgg aacttggtca taccgttgag
    11221 cgcgatagac agcagctgat gcttaatgta cgggttacgg aaacgcccgg tgactgcact
    11281 ggcgaaagat tccagttcat cacgaggcaa atccagtacc ggaataattt cttcgtagat
    11341 agctttttca acgaatgcgc aaatttcagc atcgttcatc gcttcaccta cggtatccag
    11401 ccctgcctgg aacgccaccg gcaccagcgc ggtgtgcgca ccgttgagga ttgccacttt
    11461 gcgttctttg tacggtttaa tatcgtcaac aatcagcacg ttgagcggat atttgtccag
    11521 acgcagttca gtcgctaagg atttcggtcc ctgaatcaca aacaggtaaa agtgttcagc
    11581 ggtgtcgaga aaaccatcgt gataacccag ctcttcttcc agtttagcca cttcatcgcg
    11641 cggataaccg gtaacgatac ggtccaccag cgtagaacag aagctgttag cctgatccag
    11701 ccattgaata aatgcttctg gcagtgccca ctcttgcgca tagcgcagca ccagttcacg
    11761 caacgcgtcg ccattgtagt cgatcaactc acacgggatg atgatccaac ctttatccag
    11821 cgcaccgttg aaatggctaa aacgttcgaa cagcagacgg gtcagttttg ccggataact
    11881 taccgctggc gcgtcatcga atttatcgcc cgcatggtag ctgatacccg cttcggtggt
    11941 attagagaaa acaaagcgca tttccgggtt gtgagccagt ttcaggaatt catcgtattc
    12001 actgtagacg ctgatttcac gattaaccga gcgaatcaga cgcgcgtcgc tgaccgcttc
    12061 ccccttctca ttcaggccac ggataatggt ggtatacaga ccatcctgcg tgcacagtga
    12121 cggtgggaat gaagtttcaa tcggacgaac aacgaccaca ccagaattca gatcggtgtg
    12181 ctcattcagg agatcgattt gccagtcaac aaaggcgcgc aggaagttac cttcaccaaa
    12241 ctgaatgata cgttctggat actgtgcacc gggaaaatcg cgacgattta gtgttttcac
    12301 aatgggttcc cttctgatta gtcatacaac ctgtttgaat tggtacgaca ggttagcaaa
    12361 ctttaatacg ccgaacccct gttttgatca actcctgatg attaatgagc agttttatga
    12421 gaaaagtgtg gcgcggatca tggtttaatc gaggaaatac gccttttcct ggatcataaa
    12481 gtggtagaac acattgcatt caaatcgcgc ggaatgaata aagatgtcag acaacttcct
    12541 caccgtaacg catagtgctg gtacggttgc gcccatcttt cttcgagcga tacagacacg
    12601 tatcggcttc gaccatcaat ttattaaaat catcggtcag cgttcggtac gatgcgcgac
    12661 cactaccgac gccaatactt accgtgagat aaagcgtttt ttgttgccag gtgaatggtt
    12721 gcagttcaac gcctttacgg attttttccg ccattagcag accatcgaca ggattcaccg
    12781 acggcactgc aacagcaaat tcttcgccgc ccatgcgcgc caccagcccc ttatcaccga
    12841 caatcttctg aatatgccgg gcaaacacgc ttaacacttt atcgccacat tcatgtccat
    12901 agttatcgtt aatgcttttg aaatagtcga tatcaagcag catgaccgtc agatgttgtg
    12961 tctgtttcag cgatggactt ttcagcgcct cataaagacc ggaccgtgag taaacctgag
    13021 tcagaaaatc aaagtcggct cgcagcgcaa cttgcttcat tagcgaattg atcgctgcca
    13081 cgctaaaaga aaccataatt gggcatatcg ccatcgtggc aataccgaga cgtgcggaga
    13141 acatttccgg aatggtgaac ggcgaaccga ccgagatatc aatcaccgaa tttgccacca
    13201 gtacgatttc caccgcaccg gtgacaaagg tcaacagaca tgttacctgc ggcgtatagc
    13261 gcactgcaca ccagattaat gcaggcagcg ggaacgccag actccccgct ccgccaatga
    13321 ctaccgaggc aataaccgac acaataagcg cgatggcggg catcatctgt tctgctttaa
    13381 agcgcggcag cactccagga atagccaacg tcagcataca aggcacgatc aacacgcccg
    13441 ttgagaattg ctcactgaac cagtccgcaa gcaaaggcca gaaatccaga ctatcaatac
    13501 tgaccgaacc aatcgccccg acaatagcgc ataataatgc ggcaatcaga cagtaattaa
    13561 atagccgtaa ggcacttact ggctcatact tatttttccc cagacgctta tcccgcacga
    13621 ccagtaaggc gacggtaaca ataaacatca tattggagaa attgataacc agtgaaacca
    13681 gtccccattc ggtggttatg gcatcataaa ccagcatcgc cacataactg atcgcatagt
    13741 aatgcagtcg attaagccag acatagcggg caaacacccc cgccattacg ccattcagcg
    13801 gccagaaaag agaaagttcg tgaaccagcc gtaactctgc accaataaaa tagaacagcg
    13861 tggttagcgc aaaaatagcg atgctattac gtagcagatg accttcctgg aacaaacgga
    13921 aagttgaaat ggggtgtaca tgcattaatt atctgtccat acaattgcgg gtggcaatta
    13981 tctcaacgta ataagtaaat tctcataaag gatagcatgt tcgctaacag cggcggcaaa
    14041 taaaggtacc ctgggaatgc tattttatcg ttttccgcaa ttctcatggc tgattcatct
    14101 gtacaaccag ccagtgatag attaccgcca cgatataacg ttgttgttct tcgcttaacg
    14161 aaacaccctg cggaatgtta tgccacttag ccagacaacc gcgacagcag gtcgcggtgg
    14221 cgtgctgagc gataaacacc ggatgcccgc gcatgggggt ttgcttaccg tcattagccg
    14281 gtaatgccgg agcaagacgt ttagcgacaa aatccgccgc atgttgttcg atgactggcg
    14341 cgcctttctc caggcaatac tgacgctctt tcatgcccag acgaaagcga gagcgaaatt
    14401 ttgaacggga taaacgcgcg aagagtggat cgagagactg cattaataaa ccgaacgccc
    14461 taactgtttc gtcaattgtt caaggacggc aatacccgca agcgagttac ctgcatcatc
    14521 cagttccgga ctccagacag cgatggccat ttcatgcgga acaatcgcca caataccgcc
    14581 accaacgcca gatttcgccg gtagccccac ccgccaggca aactcccccg cgttctggta
    14641 cataccactg gtcgccatca gcgcgttaat ttgccgcgcc tgcattggcg tcaccacagg
    14701 ttcatcaata tgaatagctt tcccctgatt agccagaaag acaaacgtcc gggccagctc
    14761 tacacagctc attttcagag cgcagtaatg aaagtagttt tgcagaacgg ttgtcacatc
    14821 atgatggaaa ttgccaaacg acttcatcag ccaggcgata gccgcatttc gcgcggaatg
    14881 ttcaaattcg gaacgcgcta ccaccgtatc gtatgaaata tcagacacac cgcttaagcc
    14941 gcgcacgact tccagcatac gttgccgtgg tgcgcttaat cgcccttgca acatatcgca
    15001 aaccaccagc gcaccagcat taataaacgg attacgcgga ataccctgct ccatttccag
    15061 ttgcactaaa gaattgaacg gtgatccaga cggatctttg ccgacgcgtt gccagatttc
    15121 ctcttcggag taatgacgca tggcgacaac gagactcagc actttggaaa tagactgaat
    15181 ggaaaaacgt tcttgcgcgt ctccggcctg aaaaagctgt ccgtcaacgg tacagatagc
    15241 aatgcccaat cgggaaccgt ctactgtagc cagcgccgga atataatccg cgactttacc
    15301 ctgaccaatg agcggccgca cttgccgcaa gatgttttct aaaattgcat tatccatggc
    15361 gactgccact ttctactcct ggaccgcagg tctgaaaaga cctgcgagta tatcagagct
    15421 gaatatgtcg cgtcagatcc ggtctttcca caccgtctgg atattacaga attcgtgtaa
    15481 gccgaaatgg gaaagctcac gaccaaagcc actctttttc acgccaccaa aggccactcg
    15541 cgcgtcgctg gcacaataac cattgataaa caccccaccg cattccagac gtgccgccat
    15601 ctgtctggcc tgtgtttcgt cggtggtaaa aatggtcgct gaaaggccga actcactatc
    15661 attagccagt tccagcgcat gttctgcatc tttcgcaacg gtgattgccg caacagggcc
    15721 aaacatttct tcccgaaacg cggtcatttc tggggtaaca ttcgccagaa ccgttggcgg
    15781 atagtagtta cctgccccag ccatcttttc cccgccagta acaaacgcgc accctgcgcc
    15841 agggttttct ccacctgatg atgcagctca tcacgtaaat caaaacgagc cattggtccg
    15901 agagcattct cttcatcacg gggatcgccc attttcaagg ctgccgcagc tgccacaaaa
    15961 cgttcggtaa atgcagaagc aattccctct tcgataataa agcgttttgc cgctgcacaa
    16021 acctgtccgg tattctgata acgtccggct accgccgctt taaccgccag ttccagatcg
    16081 gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt ccagtacgca ttttttcagt
    16141 gccgctccag cctgtgcgcc aatagccgct cccgcacgaa cacttccggt caccgtgaca
    16201 gcagcaatgc gcgaatcttt aatcatttga ctgacaccgt cgttgtcggc attcagccag
    16261 ccatatacgc cttgcgggat tcccgcatct ttaaacacct gggcaatgag ctgagcacag
    16321 cccatcacat tcggcgcatg tttaagtaag tagccgttac ctgcaagaat gatgggaacc
    16381 gcgccacgca tcacctgcca taacggaaag ttccacggca taatcgccag aatcgtcccc
    16441 aacggtcgat actcaataac tgcctgctga ttttccacca gcgtaggttc cgccttcagc
    16501 attgccggac catgttctgc ataccagtca cacaaattcg ccgatttcgc cacttcagcg
    16561 cgcgcctgat tgattggttt gcccatttca cgggtgatca tttgcgccat ttcttcgcta
    16621 cgggcgcgca gagccttacc gataccacgc agtttttcag cacgataatc tatatttgtc
    16681 tcgcgccagt cgcgaaagcc tgctgccgcc agctgaagtg cgttttcgat atcgttagcg
    16741 ccagcccacg gcagcacaga aagttgttca cccgtggcag gatttattga aattgcatga
    16801 gttgccggag taatggtcat cggggtatct cctttatgag tcatggtatg aagatacgca
    16861 gatttactct tgctttaaaa tgaataatat taagccactt attcacgaat cgagaatgtt
    16921 atggatctga cccaactgga gatgttcaac gcggttgccg aagctggcag cattacccag
    16981 gctgcagcaa aagtgcatcg cgtaccgtcg aatctcacta cccgtctacg ccagctggaa
    17041 acagaactgg gggtagatct gtttattcgc gagaatcagc gtttacgtct ctctcctgcc
    17101 gggcataact ttttacgcta tagccaacaa attctcgcgt tagtggatga agcgcggagc
    17161 gttgtcgctg gcgatgagcc gcaaggttta ttttctcttg gttcgctgga aagcaccgca
    17221 gcagtgcgca ttccggccac gctggcggaa ttcaaccatc gttatcccaa aattcagttt
    17281 tcactttcca ccggcccttc cggcactatg ctggaaggag tgctggaggg aaaactgaat
    17341 gcggcattta ttgatgggcc cattaaccat actgccattg acgggatacc ggtataccgc
    17401 gaggagctga tgatcgtcac gccacaaggt catgcgccag taatccgcgc cagtcaggtt
    17461 aacggcagta acatttatgc cttccgcgcc aattgttcgt atcgtcgcca cttcgagagc
    17521 tggtttcatg ctgacggtgc cgctccggga actattcacg agatggagtc gtatcacggg
    17581 atgttggcct gtgtggtcgc aggagcaggc attgcgctta ttccacgctc tatgctggaa
    17641 agtatgccgg ggcatcacca ggttgaagcg tggccgttag ctgagcaatg gcgttggtta
    17701 acaacctggc tggtctggcg tcgtggtgcg aaaacgcgtc cgcttgaggc atttattcaa
    17761 ctactggatg tgcctgactc ggcaaaacag ggttaccaat gagctatttt tgatagttct
    17821 tgcagctcaa atgccctata tagtgatgtt ccgatgactt atgactatat ggggcaaata
    17881 tggttacgcc agtaagcatc agtaattaca tatctcttcc tgatgatttt cccgtacgta
    17941 atattgcacc tcaggtaaaa gaagttttaa aggattttat tgatgcactc agtaccataa
    18001 tatgtgatga agagtggcgc acgtctttaa acaccaactc agccacaaaa aagatattta
    18061 acaaccttga taacttatca tatattcaga ggacttcttt tcggggtaac gacacactat
    18121 acaatgaaaa ggttcaattt aaactcactt attccgtcaa gaatggaaga cacaaagaaa
    18181 atattgaatt tcaggtagta ataaatttaa gccctattta tttagataat tttcgccatg
    18241 atggagaaat taatattttt gcgctcccca cccaaagcct gtcactatgg ggcgcgtatt
    18301 tcagaccggc gtcgagcgtg tgctatttct gtttctgaat gattttattg aacaatttcc
    18361 aatgatcaac cttggcgttc ccatcaaaag agcgcataca ccacatattg aacccctgtc
    18421 tccggatcgc cataccgctg cagattactt acgccagttt gatttgcttg tcctgaattt
    18481 tatctctcgc ggtaattttg tcatactccc ccgattatgg agtaactctg aggttcacag
    18541 atggttcgtc aataaagatc ctaacttgat caccgccatt ctcgacataa cagacggcga
    18601 attaaaagag gatttattgc aaagcctgat ggattcatta ggttctaaca aacatgtact
    18661 acccgaagtc tgcatctgct ttttatccct tttagcagaa caagaatctc ctcattttca
    18721 ggactttttt ttatttttcg ccaatatgtt attgcactat caccaattta tgaatcccaa
    18781 tgaaagtgat ctgaatgacg tgttgatgcc agcatcatta agtgatgata aaattatcaa
    18841 acatatggcg cgcaggaccc tcaaactgtt tgtaaaaaat gaaacgccgc caaaagttac
    18901 tcacgaagac ctggtgaaaa acaggcctcg ctcccctgtc agaccaccta tacccgcaac
    18961 cgccaaaacg ccagacctcc ctgaacgtca ttaaaccgtg atgttaccga ctctctgacg
    19021 cggaacagaa tcagcgtcag agaaacggaa aacgcgatcc agatcacaaa tgcattgtat
    19081 tcacatcatt aaccgtttta agatcatttc atcacttttt cgcaactcac ccgataatct
    19141 gttatgacaa caaacactgt ttcccgcaaa gtggcgtggc tacgggtcgt tacgctggca
    19201 gtcgccgcct tcatcttcaa caccaccgaa tttgtccctg ttggcctgct ctctgacatt
    19261 gcgcaaagtt ttcacatgca aaccgctcag gtcggcatca tgttgaccat ttacgcatgg
    19321 gtagtagcgc taatgtcatt gccttttatg ttaatgacca gtcaggttga acggcgcaaa
    19381 ttactgatct gcctgtttgt ggtgtttatt gccagccacg tactgtcgtt tttgtcgtgg
    19441 agctttaccg ttctggtgat cagtcgcatt ggtgtggctt ttgcacatgc gattttctgg
    19501 tcgattacgg catctctggc gatccgtatg gctccggccg ggaagcgagc acaggcattg
    19561 agtttaattg ccaccggtac agcactggcg atggtcttag gtttacctct cgggcgcatt
    19621 gtgggccagt atttcggttg gcgaatgacc ttcttcgcga ttggtattgg ggcgcttatc
    19681 acccttttgt gcctgattaa gttacttccc ttactgccca gtgagcattc cggttcattg
    19741 aaaagcctcc cgctattgtt ccgccgcccg gcattgatga gcatttattt gttaactgtg
    19801 gtggttgtca ccgcccatta cacggcatac agctatatcg agccttttgt acaaaacatt
    19861 gcgggattca gcgccaactt tgccacggca ttactgttat tactcggtgg tgcgggcatt
    19921 attggcagcg tgattttcgg taaactgggt aatcagtatg cgtctgcgtt ggtgagtacg
    19981 gcgattgcgc tgttgctggt gtgcctggca ttgctgttac ctgcggcgaa cagtgaaata
    20041 cacctcgggg tgctgagtat tttctggggg atcgcgatga tgatcatcgg gcttggtatg
    20101 caggttaaag tgctggcgct ggcaccagat gctaccgacg tcgcgatggc gctattctcc
    20161 ggcatattta atattggaat cggggcgggt gcgttggtag gtaatcaggt gagtttgcac
    20221 tggtcaatgt cgatgattgg ttatgtgggc gcggtgcctg cttttgccgc gttaatttgg
    20281 tcaatcatta tatttcgccg ctggccagtg acactcgaag aacagacgca atagttgaaa
    20341 ggcccattcg ggcctttttt aatggtacgt tttaatgatt tccaggatgc cgttaataat
    20401 aaactgcaca cccatacata ccagcaggaa tcccatcaga cgggagatcg cttcaatgcc
    20461 acccttgccc accagccgca taattgcgcc ggagctgcgt aggcttcccc acaaaataac
    20521 cgcaaccagg aaaaagatca gcggcggcgc aaccatcagt acccaatcag cgaaggttga
    20581 actctgacgc actgtggacg ccgagctaat aatcatcgct atggttcccg gaccagcggt
    20641 acttggcatt gctagcggca caaaggcaat attggcactg ggttcatctt ccagctcttc
    20701 cgacttgctt ttcgcctccg gtgaatcaat cgctttctgt tgcggaaaga gcatccgaaa
    20761 accgataaac gcgacgatta agccgcctgc aattcgcaga ccgggaatcg aaatgccaaa
    20821 tgtatccatc accagttgcc cggcgtaata cgccaccatc atgatggcaa atacgtacac
    20881 cgaggccatc aacgactgac gattacgttc ggcactgttc atgttgcctg ccaggccaag
    20941 aaatagcgcg acagttgtta atgggttagc taacggcagc aacaccacca gccccaggcc
    21001 aattgcttta aacaaatcta acattggtgg ttgttatcct gtgtatctgg gttatcagcg
    21061 aaaagtataa ggggtaaaca aggataaagt gtcactcttt agctaacctt gcatcgcatt
    21121 gaccaaaact tgaaccgatt tagcaaaacg tggcatcggt caattcattc atttgactta
    21181 tacttgcctg ggcaatatta tcccctgcaa ctaattactt gccagggcaa ctaatgtgaa
    21241 aagtaccagc gatctgttca atgaaattat tccattgggt cgcttaatcc atatggttaa
    21301 tcagaagaaa gatcgtctgc ttaacgagta tctgtctccg ctggatatta ccgcggcaca
    21361 gtttaaggtg ctctgctcta tccgctgcgc ggcgtgtatt actccggttg aactgaaaaa
    21421 agtgttgtcg gtcgacctgg gagcactcac ccgtatgctg gatcgcctgg tctgtaaagg
    21481 ctgggttgaa aggttgccga acccgaatga taagcgcggc gtattggtaa aacttaccac
    21541 cagcggcgcg gcaatatgcg aacaatgcca tcaattagtt ggccaggacc tgcatcaaga
    21601 attaacaaaa aacctgacgg cggacgaagt ggcaacactt gagcatttgc ttaagaaagt
    21661 cctgccgtaa acaaaaaaga ggtatgacga tgtccagacg caatactgac gctattacca
    21721 ttcatagcat tttggactgg atcgaggaca acctggaatc gccactgtca ctggagaaag
    21781 tgtcagagcg ttcaggttac tctaaatggc acctgcaacg gatgtttaaa aaagaaaccg
    21841 gtcattcatt aggccaatac atccgcagcc gtaagatgac ggaaatcgcg caaaagctga
    21901 aggaaagtaa cgagccgata ctctatctgg cagaacgata tggcttcgag tcgcaacaaa
    21961 ctctgacccg aaccttcaaa aattactttg atgttccgcc gcataaatac cggatgacca
    22021 atatgcaggg cgaatcgcgc tttttacatc cattaaatca ttacaacagc tagttgataa
    22081 cgtgacaacg tcactgaggc aatcatgaaa ccactttcat ccgcaatagc agctgcgctt
    22141 attctctttt ccgcgcaggg cgttgcggaa caaaccacgc agccagttgt tacttcttgt
    22201 gccaatgtcg tggttgttcc cccatcgcag gaacaaccac cgtttgattt aaatcacatg
    22261 ggtactggca gtgataagtc ggatgcgctc ggcgtgccct attataatca acacgctatg
    22321 tagttattgt caggctctga tcacagagcc tgacatctcc gtaatatctt cctcaagtct
    22381 caaatatttt cgccattgtt ggcgatgaga tcccgatacc agtaaaacga cgctttacgt
    22441 atccgttcca gcgttccctt cccttcgttg tctttatcga cataaatcat tccgtagcgc
    22501 tttttcattt cccctgttcc ggcagaaacc aggtcaatgc agccccacgg ggtgtagcca
    22561 attaagtcaa caccatcttc aactacggct tttttcattt cccgaatatg ggaagcaaag
    22621 taatcaatgc gatagtgatc gttcaccgtg ccgtcagctt gtctctggtc aaccgcacca
    22681 aatccatttt cgacaataaa cagcggcaac tggaaatgat cccagaacca gttgagtgaa
    22741 taacgtagcc ctgccggatc aatttgccat ccccattcgg atgtatcgat ataagggttg
    22801 ctgaccaaat ctcgtggttc aacataatcc agttgcgggt tatcgggcga aaattgtgtc
    22861 gtaaaagaca tgtagtagct aaagccgata aagtcgacac aaccactggc aagaatcgcg
    22921 ttatcttctg gtgtgatatc gaggttgaat cctttcctgg caaagtaatt cagcatatgt
    22981 tgcggataat atccacgagc atgaacatca gtaaaccagt aacgacgatg cattgctttc
    23041 gtggccatca tcatatcgtt gggtgcacac gtcagaggat agatgggaca catggcgatc
    23101 atacagccaa tgttaaattc aggattgatc gattttccag tctgtaccgc cagggcactg
    23161 gcaactaact cgtaatgtac cgcctggtac attatttgtt cgcgctcatt gatatcttcc
    23221 tctggcgaat acaagatacc ggaattagta aatggacaca ggctttcgct gaaattcacc
    23281 tgattattga tttcgttaaa cgtcatccag tactttactt tttctttata gcgcgtgaag
    23341 accgttgatg cgaagcggat aaaaaagtcg atcagtttac ggtttcgcca gccaccatat
    23401 tttgtcacca gatgataagg catctcaaaa tgtgaaagcg tcaccacagg ttccattccc
    23461 tgcttaaggc attcatcgaa cagatcatca taaaattgta aaccctcttc attcggctcc
    23521 tgttcgtcac cttgcggaaa gattcgtgtc caggcaatgg aagttcgaaa gcatttgaat
    23581 cccatctcgg caaataactg aatatctgtt ttatagcgat gataaaaatc aattgcttca
    23641 tgattgggat aattaagccc gtcgataacg ccttctgtca cttcacgcgg caccccgtga
    23701 gcgccagcag tcatcacatc agcgatgctg atgccttttc ctccttcatt ccagccacct
    23761 tccaactgat gcgcggctac cgcaccgccc cataaaaaac cttttttaaa tcctgacata
    23821 cacaactcct taactaaaat agattaccac cagacttccg cctggatgcc ggccataaac
    23881 tcgtctttgc tgttctcatt aaacttaaaa ttcgataatt cgttatccag aatattgata
    23941 tacgttccat agaagcgaat ttctggtcgt gaacctaaaa tgctttcacc cactttcaat
    24001 gcatgccaga gtgtcgtttt ataagccgat tcatttagtg ttacaccctg ctgagttttg
    24061 ttctgttgct taaaccagcc taattcaagc cccgtctgat tccatgtatt ccagatccag
    24121 gccggtctta ttacagtgcg aatactgtca aaatcactat gagcaccact ttcataacta
    24181 taaacatctt cgccgtgaga atagacaagc gcgttagcca taataatatt gtcagtaaga
    24241 tacatctcgc cctgagagat taaacgccag gcgatcccat tggtatgatc atcatagtaa
    24301 tagcgaccat gcgccatcgt attactggca tctgagaaac tggcaaaact gctggcatag
    24361 gaattattgg caacctgtaa cgtaaattca ttaaacgtat cgcgttgcag gttttgccgt
    24421 aaaacagaag tcagcatcca gctatctttc atttcaaaat aactgttgtc attttcgtta
    24481 tcttgttgtt gatccgtttt attaggtgcg gaatatttag ccattaatga taatgtcgca
    24541 tcatcccata acgggatatt gcgataacga acatccacag aattggtatt catctggctg
    24601 gtacgcgaaa aatcacgaga gtaaacatcg acatcatcac ggcttaagga catatcaaac
    24661 agccctacac caagtgccca gttttcaatc cccacacccg cggcgacatc cgtggttaag
    24721 gttttccagt ccagcatctg gatctcatat tgcgggagtt tatgtttgcc gacccagaag
    24781 tctgcctctg gtgcgaaggg taaaaaacct cgcgttgtca ggtaaatatc gctgaactgc
    24841 atgatatttt cattggcact gtcaccaaac caggcatcgt tatactgctc acccacgttg
    24901 ccatcatagg taacgaccgc atttgccgtt ttaccgtcct gattataaac acgctgattt
    24961 aaggtcaggt caaaccaacc gctcatctca ttaccaaaac gtcccagaga acccgctgca
    25021 taagtttgtg gtgagccgtg attaccagta ccccagcctg agcggaagta cccctgatag
    25081 ctgaacccaa tatcatcctt tatatattta ctgatatctt tcagggtcac gctttcgata
    25141 gtggttttgt cgcctttact ttcaataacg gcaacttgct gcgatccatt tatagtatgc
    25201 gctgcggcag acgagttctg ttcaccgttt tcatttttga tgttatcagc gacgggagat
    25261 gtcgctgata tttcagcaag cgaggctgat ttatatttgt tttccgtgat gcttttttgt
    25321 aaggtcgaaa gtcgggattt atatactccc agttcattct gtgttgcttt cagctcttgt
    25381 ttattttgcg acaattcatt ttcaagcaat tcaaggcgct gctctatggt taattttgtt
    25441 gccatcgcgt tcaatggaaa caatgctgta ataatagaaa aagacaaaag gctgacgtta
    25501 agcgtcttta tattcatgac ttatccttaa gagtataata atttcctcca gctatcgcac
    25561 tggaggaaaa aatcaggcat ggagttcttt tcttaatgaa ataatttccc tggctaaatc
    25621 ctggcataat attgcattca tcaaatgatc ctgtgcatga atcagaatta aattaacttt
    25681 tattttgcca cagccttcat cctgactaat taatgcggtc tgaatcttat gtgcctgaag
    25741 acaggcagat tcagaactgg ccattagctg agttgcgccc tgccagtctt tttgcccggc
    25801 acaacgtatg gcttccatcg cgtcagaacg tgcctgcccc gcgttgacca gtaactccat
    25861 aacaagttct tcatctgcaa acatcacaat ctccttcctg ggtaaatgga attatccgtt
    25921 agttgtcatg ttattgactt tgttggcggc aataacaaaa ggtaaataga tcattgtcgc
    25981 cacacctaaa cataaaaaac caaccagtaa agccagccag ttccccccgg ttccgaggaa
    26041 agcaattaaa ggtccgggcg tagtccaggg cacgacataa gcaaccggtg gaataatttc
    26101 catcgaaaca aagaagtaac caatggcaca gttaacaatg ggaaccataa taaacggcac
    26161 catcatgatg gggttaagta caataggaag gccaaacatt atcggttcat tgatattgaa
    26221 aataccgggg ataaatgaca tttttgccag atcacggaat tccgcacgct tagaagcgat
    26281 aaaaatagcc aacaataacc ccaacgtcat acctgaaccg ccgcagttgg cgaatgcatc
    26341 attaatagaa gtccaggtaa tcggatatgg cgcgccccag gtagtgccat gttgagcggc
    26401 ccaggagaga ttctccagat tagcctcaga aaaaacggtt tctcgaattg ccgatgtggt
    26461 attaggtccg tggatcccca gcacccacag gaaattaccc acagccccca ggataattac
    26521 cgcaaagata ttcgttccca tatgtttgag cggcgtctgg attaatgtgt aaataagatc
    26581 gtttagtcct gccggggata tcagtgccag gcaataatta agtgcggaaa agaacaccat
    26641 gacaaaaaat attggcaata aaactttaaa tgaacgcgct accgcaggcg gtacagctgc
    26701 cggcatcgtt atggtgattt ttgggtttcg agctaagcga caaaatattt cactggtgat
    26761 caatgcaacg ataacagcaa caaacaaccc ctgcgcgccg agatatttgg tcgtcaggaa
    26821 tgcttgccca tctatcatct gatatggcgt ataaacaata aaaaatgctc ctattgccgt
    26881 taatccgcat aaaagatcat cttgctcata gctaatcgcc atattcctgg cgaccaggaa
    26941 agcaatcata attgacatga tatttacaga accattcatt accggagtaa aaatagcttg
    27001 tgctttttca aggttgggga aaatgctatt cagatgcagt aatccggcaa taaaaccgtc
    27061 tggcgataat atggcaaagt taattaatat aattaatgaa cttgccatga taattggaaa
    27121 cgtaaaaacg aatccatctc tcaccgctgc aacatgtttt tgtgagttta acttgatagc
    27181 aactggaaca agaaaacgtt ccattccacg ttcgaatgag gccattaatc ccattttttt
    27241 atacctgttg ttttggatat ttatacatcc caataaagca tgtatgccat atccatttta
    27301 tgatgtgtga aaagttagtt caggtaatag tttaataata ttagattctt actcttgcag
    27361 tagtttcatc gcaaattcaa gaactgcctg tccgttcatg gtgccgtagt ctttcatctc
    27421 gataacggct acaggcattt tcccctgtgc aacggcttca atctctggtt tctgaaagcg
    27481 aacttgcggg ccaagtaaaa caacgtcaac ttcgttattt ttaattttcc ctttagcctc
    27541 tgcaatcgcc aatgcggaaa tattaacttc aagtgaaata gcgttagcat gatcaatcat
    27601 acgtttaacc agcatactgg ttgacatgcc cgcagcgcaa actaacaata tttttttcat
    27661 attttccctt actggtatat aacagattac attgtttaaa tatcatatcc cgcagtagct
    27721 atacgtcctg gtacattgat cacagtaata cagtgataaa gttgttcaac atcagggtga
    27781 ttattaaacg actcggccat acgataaacc aatcaggact caactccttg ttttcaatgg
    27841 ctcttaaaag gtgcaaaagt gatttttcaa aagattgccc gcttacttaa atccgaaatc
    27901 aatggcaatt catggcatgt tggtgatttg ttgccgtcag aagcggaact cgctgttcgt
    27961 tataatgttt cgcgtaatac cctgcgtaag gcattatcct tgctggaagg cgaaggaatt
    28021 attcacagaa agcatggttc aggaacatac attcaaaaaa agaattttgt tgcacacatt
    28081 gatcacatga acagtttcag tgaaattgcg cataaaagcg gcaaagaggc agggagtcag
    28141 attatgaaat ttgaagtgca ggatgcttca cccactattg cgactgagct gaatttagtg
    28201 actggtgagc aggtttatta cataaagaga ctgcgattca ttgaggataa tgcagcacaa
    28261 ttagaagaaa cgtggatgtc agtagcacgt tttcctgatt taaccgtgtc acatatgcaa
    28321 aaatccaaat tttcatatat tgagaatgaa tgcgggatca aaatcattgg cacctttgaa
    28381 actttctccc ctacttttcc taccccagaa atcgccagta ttttacggat cagcccacgg
    28441 gatcccatac ttaaaattca gacccaggct gtggacagta actctattcc gctggattat
    28501 tcgttacttt acagcaatat tttcgagttc caggtaaagt actttttccc gcgataaaag
    28561 cagacgcaca gagtgctgac atcacattct gtgcgtatgg tttagcctct aacctttacc
    28621 gctttacgcc accgcaagcc aaatacattg atatacagcc cggtcataat gagcaccgca
    28681 cctaaaaatt gcagacccgt taagcgttca tccaacaata gtgccgcact tgccagtccg
    28741 actacgggca ccagtaacga taacggtgca acccgccagg tttcatagcg tcccagtaac
    28801 gtcccccaga tcccataacc aacaattgtc gccacaaacg ccagatacat cagcgacaag
    28861 atggtggtca tatcaatagt aaccagactg tgaatcatgg atgcggaacc atcgagaatc
    28921 agcgaggcaa caaagaaggg aatgattggg attaaagcgc tccagattac cagcgacatc
    28981 accgccggac gcgttgagtg cgacatgatc tttttattga agatgttgcc acacgcccaa
    29041 ctaaatgctg cagccagggt caacataaag ccgagcatcg ccacatgctg accgttcaga
    29101 ctatcttcga ttaacaccag tacgccaaaa atcgctaagg cgatccccgc caattgtttg
    29161 ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga tagtaaaaaa cgcctgtgcc
    29221 tgtaacacca gcgaagccag tccagcaggc ataccaaagt taatggcgca gaacagaaag
    29281 gcaaactgcg caaaactgat ggttaatcca taccccaaga gcaaattcag tggtactttc
    29341 ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca taaagcgcaa accggccagc
    29401 atcagcggtg gcatgttatg aagccccact ttgatgacca caaaatttag cccccatacg
    29461 accactacca gtagcgccaa caccccatct tttcgcgaca ttctaccgcc tctgaatttc
    29521 atcttttgta agcaatcaac ttagctgaat ttacttttct ttaacagttg attcgttagt
    29581 cgccggttac gacggcatta atgcgcaaat aagtcgctat acttcggatt tttgccatgc
    29641 tatttcttta catctctaaa acaaaacata acgaaacgca ctgccggaca gacaaatgaa
    29701 cttatcccta cgacgctcta ccagcgccct tcttgcctcg tcgttgttat taaccatcgg
    29761 acgcggcgct acgctgccat ttatgaccat ttacttgagt cgccagtaca gcctgagtgt
    29821 cgatctaatc ggttatgcga tgacaattgc gctcactatt ggcgtcgttt ttagcctcgg
    29881 ttttggtatc ctggcggata agttcgacaa gaaacgctat atgttactgg caattaccgc
    29941 cttcgccagc ggttttattg ccattccttt agtgaataac gtgacgctgg tggtgctctt
    30001 ttttgccctc attaactgcg cctattctgt ttttgctacc gtgctgaaag cctggtttgc
    30061 cgacaatctt tcgtccacca gcaaaacgaa aatcttctca atcaactaca ccatgctcaa
    30121 cattggctgg accatcggtc cgccgctcgg cacgctgttg gtgatgcaga gcatcaatct
    30181 gcccttttgg ctggcagcta tctgttccgc gtttcccatg cttttcattc aaatttgggt
    30241 aaagcgcagc gagaaaatca tcgccacgga aacaggcagt gtctggtcgc cgaaagtttt
    30301 attacaagat aaagcactgt tgtggtttac ctgctctggt tttctggctt cttttgtaag
    30361 cggcgcattt gcttcatgca tttcacaata tgtgatggtg attgctgatg gggattttgc
    30421 cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc atggtggtta cgttgcaata
    30481 ttccgtgggc cgccgactta acccggctaa catccgcgcg ctgatgacag caggcaccct
    30541 ctgtttcgtc atcggtctgg tcggttttat tttttccggc aacagcctgc tattgtgggg
    30601 tatgtcagct gcggtattta ctgtcggtga aatcatttat gcgccgggcg agtatatgtt
    30661 gagtgaccat attgcgccgc caggaatgaa agccagctat ttttccgccc agtctttagg
    30721 ctggcttggt gccgcgatta acccattagt gagtggcgta gtgctaacca gcctgccgcc
    30781 ttcctcgctg tttgtcatct tagcgttggt gatcattgct gcgtgggtgc tgatgttaaa
    30841 agggattcga gcaagaccgt gggggcagcc cgcgctttgt tgatttaagt cgaacacaat
    30901 aaagatttaa ttcagccttc gtttaggtta cctctgctaa tatctttctc attgagatga
    30961 aaattaaggt aagcgaggaa acacaccaca ccataaacgg aggcaaataa tgctgggtaa
    31021 tatgaatgtt tttatggccg tactgggaat aattttattt tctggttttc tggccgcgta
    31081 tttcagccac aaatgggatg actaatgaac ggagataatc cctcacctaa ccggcccctt
    31141 gttacagttg tgtacaaggg gcctgatttt tatgacggcg aaaaaaaacc gccagtaaac
    31201 cggcggtgaa tgcttgcatg gatagatttg tgttttgctt ttacgctaac aggcattttc
    31261 ctgcactgat aacggatcgt tgacacagta gcatcagttt tctcaatgaa tgttaaacgg
    31321 agcttaaact cggttaatca cgttttgttc gtcaataaac atgcagcgat ttcttccggt
    31381 ttgcttaccc tcatacattg cccggtccgc tcttccaata accacatcca gaggctcttc
    31441 aggaaatgcg cgactcacac ctgctgtcac ggtaatgttg atatgccctt cagaatgtgt
    31501 gatggcatgg ttatcgacta actggcaaat tctgacacct gcacgacatg cttcttcatc
    31561 attagccgct ttgacaataa tgataaattc ttcgcccccg tagcgataaa ccgtttcgta
    31621 atcacgcgtc caactggcta agtaagttgc cagggtgcgt aatactacat cgccgattaa
    31681 atgcccgtag gtatcattaa ccaatttaaa tcgatcaata tccaacaaca ttaaataaag
    31741 attcagaggc tcagcgttgc gtaactgatg atcaaaggat tcatcaagaa cccgacgacc
    31801 cggcaatccc gtcaaaacat ccatattgct acggatcgtc agcaaataaa ttttgtaatc
    31861 ggttaatgcc gaagtaaaag aaagcaaccc ctcctgaaag gcgtcgaaat gcgcgtcctg
    31921 ccagtgattt tcaacaatag ccagcattaa ttcccgacca cagttatgca tatgttgatg
    31981 ggcagaatcc attagccgaa cgtaaggtaa ttcatcgtta tcgagtggcc ccagatgatc
    32041 aatccaccga ccaaactggc acagtccata agaatggtta tccgttattt ctggcttact
    32101 ggcatctctc gcgaccacgc tgtgaaacat actcaccagc cactggtagt gggcatcgat
    32161 agccttattg agatttaaca agatggcatc aatttccgtt gtcttcttga tcattgccac
    32221 tcctttttca cagttccttg tgcgcgctat tctaacgaga gaaaagcaaa attacgtcaa
    32281 tattttcata gaaatccgaa gttatgagtc atctctgaga taacattgtg atttaaaaca
    32341 aaatcagcgg ataaaaaagt gtttaattct gtaaatcacc tctgcattat cgtaaataaa
    32401 aggatgacaa atagcataac ccaataccct aatggcccag tagttcaggc catcaggcta
    32461 atttattttt atttctgcaa atgagtgacc cgaacgacgg ccggcgcgct tttcttatcc
    32521 agactgccgc taatgttgat catctggtcc ggctgaactt ctcgtccatc aaagacggcc
    32581 gcaggaataa cgacattaat ttcaccgctc ttatcgcgaa aaacgtaacg gtcctctcct
    32641 ttgtgagaaa tcaaattacc gcgtagtgaa accgaagcgc catcgtgcat gttttttgcg
    32701 aaatcaacgg tcattttttt tgcatcatcg gttccgcgat agccatcttc tattgcatga
    32761 ggcggcggtg gcgctgcatc ctgttttaaa ccgccctggt catctgccaa cgcataaggc
    32821 atgacaagaa aacttgctaa tacaatggcc tgaaatttca tactaactcc ttaattgcgt
    32881 ttggtttgac ttattaagtc tggttgctat ttttataatt gccaaataag aatactgcca
    32941 attgttataa ggcatttaaa atcagcccac tagctgtcaa atattcagag aatttaactc
    33001 actaaagtta agaagattga agagtcttaa acatattttc agaataatcg gatttatatg
    33061 tttgaaaatt attatatttg acgagcatac agaaaaagca aatcaccttt acatataata
    33121 gcgtggacaa aaaacagtga acattaataa agataaaatt gtacaactcg cagataccga
    33181 cactattgaa aacctgacat ccgcgttgag tcaaagactt atcgcggatc aattacgctt
    33241 aactaccgcc gaatcatgca ccggcggtaa gttggctagc gccctgtgtg cagctgaaga
    33301 tacacccaaa ttttacggtg caggctttgt tactttcacc gatcaggcaa agatgaaaat
    33361 cctcagcgta agccagcaat ctcttgaacg atattctgcg gtgagtgaga aagtggcagc
    33421 agaaatggca acaggtgcca tagagcgtgc ggatgctgat gtcagtattg ccattaccgg
    33481 ctacggcgga ccggagggcg gtgaagatgg tacgccagcg ggtaccgtct ggttcgcgtg
    33541 gcatattaaa ggccagacct acactgcggt tatgcatttt gctggcgact gcgaaacggt
    33601 attagcttta gcggtgaggt ttgccctcgt ccagctgctg caattactgc tataaccagg
    33661 ctggcctggc gatatctcca ggccagccat tggtggtgtt tatatgttca agccacgatg
    33721 ttgcagcatc ggcataatct gaggagcctt accgcgccat tgtcgataca ggcgttccag
    33781 atcttcgctg ttacctctgg aaaggatcgc ctcgcgaaaa cgctgcccat tttcacgcgt
    33841 taatccgccc tgctcaacaa accactggta accatcatcg gccaacattt gcgtccacag
    33901 ataagcgtaa taacctgcag catatccgcc accaaaaata tgggcgaaat aactgctgcg
    33961 atagcgtggc ggtatagcag gaagatccat attttccgcc accagcgccc gcaattcaaa
    34021 atcatcgaca tcctgcattg cttcgttttc ttccaggcaa tgccagcgca tatcgagaag
    34081 tgcggcgcta agcagttcgc tcatctcata ccctttgttg aacaggctgg cattacgcat
    34141 tttctgttgc agttcgtcag gcattgctgc cccgctctga taatgccggg cgtagcgagc
    34201 gaatacctgc ggatgcgttg cccagtgttc gttgatttgc gacggaaatt cgacaaaatc
    34261 acgcggcgtg ttggtgccgg aaagcgtggc ataacgctgg cgggcaaaaa ggccgtgcaa
    34321 cgtatgacca aattcatgga ataaggttat gacatcatcc cagagtaaca acgcaggctc
    34381 accggcagcg ggtttctgat aattgcaaac gttataaatt accggatgcg tttcattaag
    34441 cgttgattgc tcaacaaaat tgcccatcca tgcaccgccg ctttttgaat cacgggcgaa
    34501 gaaatcaccg taaaataacg ccagccccac gccattatga tcaaaaattt cccacacccg
    34561 aacgtcagga tggtagacag gaatatcaaa acgttcgaca aacttaatac cgaagagctg
    34621 attcgcggtc cagaatacac cttcattcaa caccgtgttt aattcaaaat atggcttgag
    34681 ctgcgcctca tcaagatcaa atttctcccg ccgtacctgt tcggcataaa atgcccagtc
    34741 ccacggctgc gcgctaaacc cgccctgctg cttatcaata accgcctgta tggaggctaa
    34801 ttcatcgctc gcacgttgac gcgccgctgg aacaatttcc cgcataaagt tgagcgctgc
    34861 ttcaggtgtt tttgccatct gatcggcgat tttccatgcg gcataatgag gaaaaccaag
    34921 taatgtcgcc tgttgtgcac ggatctccac cagacgttga atgatagcgc gggtatcatt
    34981 gccatcattt ttttccgctc gcgtccagcc cgcaataaac agtttttcac gcgtcgcacg
    35041 atcgcgcatt tcggcaagcg ccggttgttg ggtggtattc agcagcggaa tcagccattt
    35101 gttatccaga cctttctcgc gagccgcctc tgccgccagc gcaatctctt gctcactcat
    35161 tcctgccagc tgcgcgatat cgttcacaac cagaccgccg gatttatttg ctgccagtaa
    35221 ccgctggtta aactgactgg tcagtgtcgc agcttctgta ttcagtactt ttaattttgc
    35281 tttatcagct tgcgcaagtt tggctccggc aaggacaaaa cgttgatgaa tcacctccac
    35341 caggcggatg gattcactat caagccccag ggattcacgg cgctgccaga cagcatctac
    35401 ccgcgcgaat aattcaccgt tcagatagat atcattagcc agttccgcca gttcagcgga
    35461 aaactgttca tcaagacgct gtaattcatc attggtatgc gccgcagtca tcgcaaaaaa
    35521 gacgctggta acgcgggtaa gtaattctcc gctttgttcc agtgccagaa tagtattgtt
    35581 gaaatcaggc gtttgcgggt taagcgcgat ggcagcaatt tctgcccgct tttgctgcat
    35641 tccctcatcg aatgccgggc gatagtgatg attggcaatt tgatcaaaat gaggagccag
    35701 atacggcagt gtgctttgca caaggaaagg attcattgtt gtcattttct tctcctgaac
    35761 gcgaggtgtt ccatagcgta ggcttactga taacgtagtg caatcttgca aaccagtatt
    35821 acccgctctt aagcatcacg tgctatgtta ttgacacaca aaagcgttga ggaacagtga
    35881 gatgatcgtt ttagtaactg gagcaacggc aggttttggt gaatgcatta ctcgtcgttt
    35941 tattcaacaa gggcataaag ttatcgccac tggccgtcgc caggagcggt tgcaggagtt
    36001 aaaagacgaa ctgggagata atctgtatat cgcccaactg gacgttcgca accgtgccgc
    36061 tattgaagag atgctggcat cgcttcctgc cgagtggtgc aatattgata tcctggtaaa
    36121 taatgccggc ttggcgttgg gcatggagcc tgcgcataaa gccagcgttg aagactggga
    36181 aacgatgatt gataccaaca acaaaggcct ggtatatatg acgcgcgccg tcttaccggg
    36241 tatggttgaa cgtaatcatg gtcatattat taacattggc tcaacggcag gtagctggcc
    36301 gtatgccggt ggtaacgttt acggtgcgac gaaagcgttt gttcgtcagt ttagcctgaa
    36361 tctgcgtacg gatctgcatg gtacggcggt gcgcgtcacc gacatcgaac cgggtctggt
    36421 gggtggcacc gagttttcca atgtccgctt taaaggcgat gacggtaaag cggaaaaaac
    36481 ctatcaaaat accgttgcat tgacgccaga agatgtcagc gaagccgtct ggtgggtgtc
    36541 aacgctgcct gctcacgtca atatcaatac cctggaaatg atgccggtta cccaaagcta
    36601 tgccggactg aatgtccacc gtcagtaatt tttatacccg gcgtaactgc cgggttattg
    36661 cttgtcacaa aaaagtggta gactcatgca gttaactcac tcacaagcaa gaacgaatga
    36721 ccgtcgaaac gcaacttaat cccacacagc ctgtcaatca gcaaatttat cgtatccttc
    36781 gtcgcgacat tgtccattgc ctgattgctc caggcacacc gttgtcggaa aaagaagttt
    36841 ctgttcgttt caatgtgtca cgccagccgg ttcgtgaagc ctttattaaa ctggcggaaa
    36901 acggcctgat tcaaattcgt ccacagcgtg gcagctacgt caacaaaatt tccatggccc
    36961 aggtgcgcaa cggcagtttt atccgtcagg ccattgagtg cgcggtggcg cgtcgggcgg
    37021 cgagcatgat taccgaaagc cagtgctacc aactggaaca aaatcttcac cagcaacgca
    37081 ttgccattga gcgcaagcaa ctggatgatt tttttgaact tgatgacaac ttccatcaac
    37141 tcctgacgca gattgccgac tgtcaactgg cgtgggatac cattgagaac ctgaaagcga
    37201 ccgttgatcg cgtgcgctat atgagtttcg accatgtttc tccaccagaa atgctgttac
    37261 gccagcatct tgatattttc tctgccctgc aaaaacgtga tggcgatgcg gtagaacgtg
    37321 caatgacgca acatttgcag gaaatcagcg aatccgtgcg ccagatccgc caggaaaaca
    37381 gcgactggtt tagcgaagag taattcattt actctcatcc catccgggat gagaggcttt
    37441 tccccccgcc ttacggctca tgcatgcatc aaaaaagatg tgagcttgat caaaaacaaa
    37501 aaatatttca ctcgacagga gtatttatat tgcgcccgtt acgtgggctt cgactgtaaa
    37561 tcagaaagga gaaaacacct atgacgacct acgatcgtaa ccgtaacgca atcaccactg
    37621 gcagccgtgt tatggttagc ggcaccggtc acactggcaa gatcctgtcg attgatactg
    37681 aaggtctgac cgctgagcaa atccgccgcg gaaaaaccgt agttgttgaa ggttgtgaag
    37741 agaaactggc acctctggac ctgattcgtc tcggcatgaa ctaagcgtgt gaatgccgcc
    37801 gatggcggca ttgctttttt tacttcacgg aatatttcgc cacggtcgct ttcgcgccat
    37861 gcgctaataa agacaagtac gcttccgtca cttttgcagt aaacaagcta ttgtctggca
    37921 aatcatcacc aaagattgcc ttaatcgcca gcaatgactg gacgcgcgct ttcccttcgg
    37981 cactactttg tacagccttc tgaataacag gtaacagtgg gtcactgatt tctatcggat
    38041 ttccctgttc atcaacacca ccgacataac gcatccaacc cgcgacgccc agcgccagca
    38101 gatcgaactt gctgtcatgc gccagatgcc agcgaacaga atccaacatc cgctgtggca
    38161 atttctggct accatccatc gcaatctgcc aggttcgatg acgtaacgcc gggttgctat
    38221 agcgttcaat taatcggtta gcgtaatctt gcaaatcaac gccctgcact ttcagcgtcg
    38281 gcgcttgttc ctgcaacatc aagccatacg ccgcataacg ataatgttca tcttccatgc
    38341 agtcattaat gtgctgatat ccagcaagat aacccaaata cgccaggaat gaatgactgc
    38401 cgttgagcat gcgcaacttc atctcttcat aaggcagcac atcactaacc agttcggctc
    38461 ccgctttttc ccattctgga cgtccggcaa caaagttatc ttctattacc cactggcgga
    38521 aaggttcaca ggcaacgccc gcaggatcgc ccacaccggt aagttgttcg attttcgcca
    38581 gcgtatcctc tgtcactgcg ggcacaatac ggtccaccat tgttgatggg aaagtcacgt
    38641 tatcttcgat ccattgtgcc agtttttcat caacggcttg cgcgtaggaa gtgacaacgt
    38701 cacgcataac atgaccgttt tctggcatgt tgtcacatga catgacggta aatgcgggaa
    38761 gtcctgccgc tttacggcga gccagcgcct caacaatcac tcctgttgct gttttcggct
    38821 ggtggggatt ttgcacgtcg gcagcgacca tcgggtgatc gagcattaac tgtccggtcg
    38881 ccggagagtg gaaataccct ttttcggtga ttgtcagaga gacaatcgcg atttgcggtt
    38941 cacacatcgc agccaacacg gtttctaagc catccatctg tacgtgcaag gcttttttaa
    39001 cgacgccaac gacgcgagcc gtccacgcat cggccgacat ttccgcaacg gtataaagat
    39061 tatcttgctg ttgtaaatcg gcaatttgct gttcgccgcc gattaagttg acctcataat
    39121 atccccagtc actgaaatgt tctgtagcaa gaatatcggc atacacgccc tgatgcgcac
    39181 ggtgaaatgc accaaagcct aaatgaataa ttcttggggc caggttatta cgatcataaa
    39241 cagggagtgt cgcttttgct gataacaaat tatttcccat aacaattcct taaatataaa
    39301 tatggcaagc tatatgtttt gttatatgaa taaaaatccc ctctccggta agagaaggga
    39361 ttgaggattt acagacttct ggaaggttgc gcagctctta caacacgcgg ttgatcttcc
    39421 gcagcgtctt ccagcgcact taaatcacgg tctttcacct ctggcatttt cagcgcagag
    39481 attaaaccaa ttactgaata tgccatgatc ataatggcga tcggatacca ggattccgtc
    39541 atggtgcaga aaatacccgc caggatagga ccaaaaccgg aagcgataag accaccaatt
    39601 tctttagaaa tagccatccg ggtaaagcgg tttttacagc cgaacatttc tgccatggta
    39661 atgttttcca gagcaaataa tcccagcacc gcacagttat gaatcacaat cagtgcaacc
    39721 ataatggtgc tcggggcata gcttttatct acaatgatag aaagcattgg ccatgccagc
    39781 acaatcgcgg aggtattcat aataatatac gggatccggc gaccaatttt atcggataac
    39841 caaccaagga acggaatggt cataaagccg agaatcgaac taatcatcaa tgcatctgtt
    39901 ggaattgctt tgttaaacaa taacgtctgc actaaatagc ctgcaaggaa agtctgaatt
    39961 aacccagagt tacccgcctg accaaaacgc agccctgttg ccagccagaa ggatttgctc
    40021 tggaacatgc taccagcagg tgcaggtttt gctgtgggtt ggttgctgtc attaaccttc
    40081 tcaaagaccg ggctttcttt cagattcata cgtaaccaga tagcaaagac catcacgaca
    40141 acgctcgcca ggaacggtat acgccatccc cacgccagca gttcctcttt actgagaatg
    40201 aagaacataa aggcccagat tgccgttgcg ctcaaggttc cgcagttagt tcccatagct
    40261 acaaatgagg agataattcc gcgcttacct tttggtgcat attccgccag catcgtaccg
    40321 gcaccggaaa tttccgcacc tgcacccaac ccctgaataa tacgcagcgt taccagcaag
    40381 atgggggcaa aaacaccaat ctgtgcatag gtcggtaaca caccaattaa ggtggtacag
    40441 atccccatca tggtgatggt aataaagagc acttttttac gccctattct gtcgcccatt
    40501 ttgccgaaaa taaatgctcc gacaatacgc gccacataac ctgcaccgta ggttcccatt
    40561 gccaaaatta acgccattgc cgttgatgat tcaggaaaaa atatttcatg aaacactaac
    40621 gctgcgccga gcgaatataa ctggaaatcc ataattcaca ggtgtttttt cccatcctgt
    40681 ggtttccttg gcgttttcta ggttttttca gatagttgca ttttttaaaa agcatcctaa
    40741 gttcgatctc agtgtctatc tggggcctat ttctgtccca tatatgcccc aaaaaaactc
    40801 cccaacagat aagtagtttt ttcatggatt tatgcgtaaa atcaagaacg gctggaaatc
    40861 attcaatact cacactatcg aaaaatttac cagccaaccg cagcacgttc ttgcataagg
    40921 cgtgtctgcg gtttttcaac tattcagata catcactccc atcacattca ttcctccgca
    40981 tcaaaggcat ataggctata tcaccttgat atttttcttt ttcagataaa aactgttatc
    41041 tatgtatact tttaaaccca atccgtgtag agtctctgca taagatagtt tgcagttgcc
    41101 gcttcagctt gcgccataaa ccgcctgatt tttgctgcca cctgttagca ttcctgtata
    41161 cctgaaacga caatgtttat ctacgaactt taagaacacc caagataaaa attgtcaact
    41221 atatcatata taacacatta ctaattcgag gctatatgaa cagcatactg ataatcactt
    41281 ctctccttat catattcagc atttttagtc atgccctaat aaaattaggg attggcatat
    41341 ccaataaccc agacaaaacc gatgtataag tcaacatatc ctgaatcaga catacaatat
    41401 cgcaatgaaa atcaataata ttttaaggaa tatcttcatg aaatcaaaag acaccctaaa
    41461 gtggttccct gcgcagcttc ctgaagtaag aattatccta ggggatgctg tagtggaagt
    41521 agcaaaacag ggaagaccta tcaataccag aacattgctt gattacattg aaggaaacat
    41581 aaagaaaaaa tcatggctgg ataacaaaga attattacaa acagcgatat cagttcttaa
    41641 agacaaccaa
//
LOCUS       DAZGSP010000041        41608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZGSP010000041 DAZGSP010000000
VERSION     DAZGSP010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.041"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            234..1331
                     /gene="mltA"
                     /locus_tag="ACHK74_002288"
     CDS             234..1331
                     /gene="mltA"
                     /locus_tag="ACHK74_002288"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFP0221101.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1408..2214
                     /gene="tcdA"
                     /locus_tag="ACHK74_002289"
     CDS             1408..2214
                     /gene="tcdA"
                     /locus_tag="ACHK74_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFP0221102.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2265..2708)
                     /gene="csdE"
                     /locus_tag="ACHK74_002290"
     CDS             complement(2265..2708)
                     /gene="csdE"
                     /locus_tag="ACHK74_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFP0221103.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TVAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2708..3913)
                     /gene="csdA"
                     /locus_tag="ACHK74_002291"
     CDS             complement(2708..3913)
                     /gene="csdA"
                     /locus_tag="ACHK74_002291"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFP0221104.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATQQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4105..4332
                     /gene="ygdI"
                     /locus_tag="ACHK74_002292"
     CDS             4105..4332
                     /gene="ygdI"
                     /locus_tag="ACHK74_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFP0221105.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4683..5600
                     /gene="gcvA"
                     /locus_tag="ACHK74_002293"
     CDS             4683..5600
                     /gene="gcvA"
                     /locus_tag="ACHK74_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFP0221106.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5619..6014
                     /gene="ygdD"
                     /locus_tag="ACHK74_002294"
     CDS             5619..6014
                     /gene="ygdD"
                     /locus_tag="ACHK74_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFP0221107.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6007..7107
                     /gene="rlmM"
                     /locus_tag="ACHK74_002295"
     CDS             6007..7107
                     /gene="rlmM"
                     /locus_tag="ACHK74_002295"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFP0221108.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7151..7882)
                     /gene="fucR"
                     /locus_tag="ACHK74_002296"
     CDS             complement(7151..7882)
                     /gene="fucR"
                     /locus_tag="ACHK74_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFP0221109.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7940..8362)
                     /gene="fucU"
                     /locus_tag="ACHK74_002297"
     CDS             complement(7940..8362)
                     /gene="fucU"
                     /locus_tag="ACHK74_002297"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFP0221110.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8364..9782)
                     /gene="fucK"
                     /locus_tag="ACHK74_002298"
     CDS             complement(8364..9782)
                     /gene="fucK"
                     /locus_tag="ACHK74_002298"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFP0221111.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9891..11666)
                     /gene="fucI"
                     /locus_tag="ACHK74_002299"
     CDS             complement(9891..11666)
                     /gene="fucI"
                     /locus_tag="ACHK74_002299"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFP0221112.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11699..13015)
                     /gene="fucP"
                     /locus_tag="ACHK74_002300"
     CDS             complement(11699..13015)
                     /gene="fucP"
                     /locus_tag="ACHK74_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFP0221113.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13562..14209
                     /gene="fucA"
                     /locus_tag="ACHK74_002301"
     CDS             13562..14209
                     /gene="fucA"
                     /locus_tag="ACHK74_002301"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFP0221114.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14237..15385
                     /gene="fucO"
                     /locus_tag="ACHK74_002302"
     CDS             14237..15385
                     /gene="fucO"
                     /locus_tag="ACHK74_002302"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFP0221115.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15440..16195)
                     /gene="xni"
                     /locus_tag="ACHK74_002303"
     CDS             complement(15440..16195)
                     /gene="xni"
                     /locus_tag="ACHK74_002303"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFP0221116.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLKQLRLVR"
     gene            complement(16307..17674)
                     /gene="sdaB"
                     /locus_tag="ACHK74_002304"
     CDS             complement(16307..17674)
                     /gene="sdaB"
                     /locus_tag="ACHK74_002304"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFP0221117.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17732..19021)
                     /gene="sdaC"
                     /locus_tag="ACHK74_002305"
     CDS             complement(17732..19021)
                     /gene="sdaC"
                     /locus_tag="ACHK74_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFP0221118.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(19578..20942)
                     /gene="ppnN"
                     /locus_tag="ACHK74_002306"
     CDS             complement(19578..20942)
                     /gene="ppnN"
                     /locus_tag="ACHK74_002306"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFP0221119.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(21054..21902)
                     /gene="queF"
                     /locus_tag="ACHK74_002307"
     CDS             complement(21054..21902)
                     /gene="queF"
                     /locus_tag="ACHK74_002307"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFP0221120.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            21970..22515
                     /gene="syd"
                     /locus_tag="ACHK74_002308"
     CDS             21970..22515
                     /gene="syd"
                     /locus_tag="ACHK74_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFP0221121.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            23137..23466
                     /gene="yqcC"
                     /locus_tag="ACHK74_002309"
     CDS             23137..23466
                     /gene="yqcC"
                     /locus_tag="ACHK74_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFP0221122.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            23466..24248
                     /gene="truC"
                     /locus_tag="ACHK74_002310"
     CDS             23466..24248
                     /gene="truC"
                     /locus_tag="ACHK74_002310"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFP0221123.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            24266..24715
                     /gene="yqcA"
                     /locus_tag="ACHK74_002311"
     CDS             24266..24715
                     /gene="yqcA"
                     /locus_tag="ACHK74_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFP0221124.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            25150..26502
                     /gene="gudP"
                     /locus_tag="ACHK74_002312"
     CDS             25150..26502
                     /gene="gudP"
                     /locus_tag="ACHK74_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFP0221125.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            26504..27844
                     /gene="gudX"
                     /locus_tag="ACHK74_002313"
     CDS             26504..27844
                     /gene="gudX"
                     /locus_tag="ACHK74_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFP0221126.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPVGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            27865..29205
                     /gene="gudD"
                     /locus_tag="ACHK74_002314"
     CDS             27865..29205
                     /gene="gudD"
                     /locus_tag="ACHK74_002314"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFP0221127.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLSDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(29437..32193)
                     /gene="barA"
                     /locus_tag="ACHK74_002315"
     CDS             complement(29437..32193)
                     /gene="barA"
                     /locus_tag="ACHK74_002315"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFP0221128.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            32250..33551
                     /gene="rlmD"
                     /locus_tag="ACHK74_002316"
     CDS             32250..33551
                     /gene="rlmD"
                     /locus_tag="ACHK74_002316"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP0221129.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            33599..35833
                     /gene="relA"
                     /locus_tag="ACHK74_002317"
     CDS             33599..35833
                     /gene="relA"
                     /locus_tag="ACHK74_002317"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFP0221130.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            35911..36159
                     /gene="mazE"
                     /locus_tag="ACHK74_002318"
     CDS             35911..36159
                     /gene="mazE"
                     /locus_tag="ACHK74_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFP0221131.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            36159..36494
                     /gene="mazF"
                     /locus_tag="ACHK74_002319"
     CDS             36159..36494
                     /gene="mazF"
                     /locus_tag="ACHK74_002319"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFP0221132.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            36565..37356
                     /gene="mazG"
                     /locus_tag="ACHK74_002320"
     CDS             36565..37356
                     /gene="mazG"
                     /locus_tag="ACHK74_002320"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFP0221133.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            37584..39221
                     /gene="pyrG"
                     /locus_tag="ACHK74_002321"
     CDS             37584..39221
                     /gene="pyrG"
                     /locus_tag="ACHK74_002321"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFP0221134.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            39309..40607
                     /gene="eno"
                     /locus_tag="ACHK74_002322"
     CDS             39309..40607
                     /gene="eno"
                     /locus_tag="ACHK74_002322"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFP0221135.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(40667..41539)
                     /gene="ygcG"
                     /locus_tag="ACHK74_002323"
     CDS             complement(40667..41539)
                     /gene="ygcG"
                     /locus_tag="ACHK74_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP0221136.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVYISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
BASE COUNT        10339 a        10064 c        11029 g        10176 t
ORIGIN      
        1 agctaccagg ctgctccacc ccgcgtccgt ggatgcgcac tatactccgc tcgcgttttc
       61 atgcaacctt ttttttaccc cgatcacgga tttagcatga ttttgaacaa aaacaaacca
      121 ctaaagccta ttttttgtgc aaaatttgcg tcagggagag gctgctcatt tcattaaacg
      181 cggcggtttg ttatcttcgt tgcgccttat tttttaacct gaagaagaga acaatgaaag
      241 gacgttgggt aaagtacctt cttatgggca cggttgtggc aatgcttgcc gcctgctctt
      301 ccaaaccaac cgatcgcgga cagcaatata aagacgggaa atttacccag cctttctctc
      361 tggtgaacca gccagatgcc gttggcgcgc cgattaacgc cggtgatttt gccgagcaaa
      421 ttaaccatat ccgtaattcg tcaccgcgtc tgtatggcaa ccagagtaat gtttataacg
      481 cggtgcaaga gtggctgcgc gcaggcggtg atacccgcaa tatgcgccag ttcggcattg
      541 atgcctggca gatggaaggt gccgacaact atggtaacgt gcagtttaca ggctattaca
      601 cgccggtaat tcaggcgcgc catacccgcc agggcgagtt ccagtatcct atttaccgta
      661 tgccgccaaa acgtggtcgt ctgccgtctc gtgcggagat ctacgcaggg gcgctgagtg
      721 ataaatatat tctcgcttac agcaactccc tgatggataa cttcattatg gatgtgcagg
      781 gtagcgggta tatcgacttt ggtgatggca gtccgcttaa ctttttcagc tatgcaggga
      841 aaaacggtca tgcctatcgc agcattggta aggtgctgat cgaccgtggc gaagtgaaaa
      901 aagaagatat gtcgatgcag gcgattcgtc attggggcga aacacacagt gaagccgagg
      961 ttcgcgagct gctggaacag aacccgtctt tcgttttctt taaaccgcaa tcttttgcac
     1021 cggtgaaagg ggcaagtgcg gtgccgctgg ttggccgtgc ttctgttgcc tccgatcgtt
     1081 caattattcc gccaggtact accttgctgg cagaagtgcc gctgctggat aataacggca
     1141 aatttaacgg tcagtacgaa ttgcgtctga tggtggcgct ggatgtcggt ggcgcaatca
     1201 aaggccaaca cttcgatatc tatcaaggga tcgggccgga agccggacat cgcgcaggtt
     1261 ggtacaacca ctatggacgt gtctgggtgc tgaaaaccgc cccgggcgca ggtaacgtct
     1321 ttagcggctg atgtgttatt ctgtataccg tttttacgga tacgccaggg tgagggataa
     1381 cctcaccctt tttaattctg aggtgctatg tctgtggtaa ttagtgatgc atggcgtcag
     1441 cgttttggtg gcacagctcg tctgtatggt gaaaaagcgt tgcaactgtt tgctgacgcg
     1501 catatttgtg tggttggtat cggtggtgtc ggttcctggg cggcggaagc gctggcgcgc
     1561 acggggattg gcgcaatcac gcttatcgat atggatgatg tgtgcgtcac caataccaat
     1621 cggcaaattc acgctctgcg cgataacgtc gggctggcaa aagcggaagt catggcggag
     1681 cgtattcgcc agattaaccc ggagtgccgt gtaacggtgg tggatgattt cgtgacgccg
     1741 gataacgtag cgcagtatat gagcgtgggt tattcgtacg tgattgatgc cattgatagt
     1801 gtacggccca aagcggcgct gattgcttat tgtcggcgca ataaaatccc gctggtcacg
     1861 accggtggcg cgggtgggca gattgacccg acgcagattc aggttaccga tctggcgaaa
     1921 acgattcagg atccgctggc ggcgaagttg cgcgagcgcc tgaaaagcga ttttggcgta
     1981 gtgaaaaaca gtaaaggtaa gctcggcgtc gattgcgtgt tttctactga agcgctggtg
     2041 tacccgcagt cagacggtac tgtgtgtgcg atgaaagcca cggcagaagg gccgaagcgg
     2101 atggattgtg catctggatt tggcgcggca acgatggtga ccgccacctt tgggtttgtt
     2161 gcggtttctc atgcgctgaa gaagatgatg gcgaaagcgg cgcgtcaggg ttaaacaatc
     2221 aggtcggatg cgacgcgaac gtcttatccg acctggttct gtgcttaaac ctgcttcgcc
     2281 gcagcgataa tcgcctcgct taacgcattc aacccctggc tgcgtgaggc gctaagctgt
     2341 gcacgcaatc ccagctcatc aaacaatgcc agcggcgact gtgcctgcaa ttcggcgacg
     2401 gttttcccct caacggcagt caacaacacc gccaacaggc cgcgcacaat gcgcccttcg
     2461 ctgtcgccaa agaagtgcat tttaccgttt tcagtcacgg tatatcccag ccagacgcgg
     2521 ttttcgcatc cggcaatctc tttagcctgc gcttttaact cgtctggcaa agccggaagc
     2581 tgtttcccca gcatgatcaa ctggcgatat ttatcttccc attgcgacaa cggtgcgaag
     2641 gtattgcgta acgtttctgc ggttacggtt gtgccgaacg gatgtccggc gaattgcggg
     2701 tttgtcatta atccaccaat aattccagcg cgcggtcaac ggcattcacc agcgcatcca
     2761 catcactctt tgtattatat ggcgcaaaag aggcgcgcag tgtgccggtt acgcctaatt
     2821 ctgccagtag cggctgagcg caatgctgcc cggcccgcag ggcaataccg tactccgcca
     2881 gcagcgtcac catatcgcta tgatgaacgc cagcaaaatc aaaggccagc aggctggaat
     2941 cctggcagcg gaatgaacga aagccgggac gtttcgccag cgcatcttcc gccagcgttg
     3001 ctaagctacg gctccagctt tcggcctggt tgatatcgta atctgccagc cattccagcg
     3061 ccgcgcttaa tcctatgaca ccagcgacat ttggcgttcc agcttccagt ttccacggcg
     3121 cagattgagt cgtgaagccg tcaaaactca cttcgtgaac cattttgccg ccgcccagcc
     3181 agggcgacat cgcctccagc agttctgatt taccatacag cacgccgata cctgtcgggc
     3241 catacagttt gtgacctgaa aaagcataga aatcaatatc cagttgctga acatccgcgg
     3301 ggaaatgcac tgccccctga gcaccatcaa ccatcaccac catcccggct gaatgagcaa
     3361 aggtaatcgc tcgcgccaga tccgggcaac cgccagtaac gttcgacatc tgacccaacg
     3421 ccagaatccg actacgggga gtaatcagtt ctggcaacaa atcgacatcc ggcagtcgct
     3481 gcgcattaag cggcaatttc accactttgg ctccagtttg ttgggcgacc atcagccagg
     3541 ggacgaggtt ggcgtggtgt tctgccacgc tgacaataat ctcatcgccc ggttgcagac
     3601 gcggacgcgc atagcattgt gccaccatgt tgatggattc agtggtgccg cgcgtccaga
     3661 cgatagtttt atcatccggt gcattcagta attgcgccac tttctctcgt gcagcttcat
     3721 aacgcgcggt caggcgttgg gcttcggcaa actggctgcg atggacgttt ccggcgctca
     3781 gactgtaaaa ctgttgggtg gcttcaacca cggcttcagg tttaagcgcg gtcgcggcgc
     3841 tgtcgagata gacgcccgca tcctgtagtg cgggaaactg ggcgcgaaac tgcgcgggat
     3901 taaaaacgtt catggtactc ctcggcttga tgcaccgatc gtgacgcaaa attcactttg
     3961 atacaaggag agtgctaacc atcagaatta tctggatatc ctggcgaagc cgcgcaagta
     4021 acatatgctg aatagtgtta ggcgttaatt ttagcctgtt aatcaatact attaagattt
     4081 attaatcgct ttaaggagaa gatgatgaaa aagactgccg caattatttc tgcctgtatg
     4141 ctgacttttg ccctgagcgc ctgttccggt tcgaactatg tgatgcacac caatgacgga
     4201 cgtaccatcg tctctgacgg caaaccacag actgataacg ataccggtat gatttcgtat
     4261 aaagacgcta atggcaacaa acagcagatc aaccgtactg acgtgaaaga gatggtcgaa
     4321 ctggatcagt aattcgcgat cgcaaggtaa aaaaaagcac cgcaattagg cggtgctaca
     4381 ttaatcacta tggacagaca gggtaaatgt acaggaagtg aaaaaaggta gctttgctac
     4441 catggtctga atcgcagacc aattgcaaac acaacaacac aacatcacaa ccgtaagcca
     4501 aaagttcacc agaacacgca ttccgataaa acttttcgtt ccggctcagg aagtgccgcc
     4561 actataggta tttgctggta gaagctcaac ggacaattta taatggctca gattaaaaaa
     4621 actaataggt tacacagtgt gatctaattg ttaaattcat ttaacatcaa agtttaaaag
     4681 ccatgtctaa acgattacca ccgctaaatg ccttacgagt ttttgatgcc gcagcacgcc
     4741 atttaagttt cactcgcgca gcagaagagc tttttgtgac ccaggccgca gtaagtcatc
     4801 aaatcaagtc tcttgaggat tttttggggc taaaactgtt ccgccgccgt aatcgttcac
     4861 tcctgctgac cgaggaaggg caaagctatt tcctcgatat caaagagata ttttcgcaat
     4921 taaccgaagc gacgcgtaaa ctccaggccc gtagcgccaa gggggcgttg acggtcagtt
     4981 tactccccag tttcgccatt cattggttgg ttccgcgact ttccagcttt aattcagctt
     5041 atccgggaat tgacgttcga atccaggcgg ttgatcgtca ggaagataag ctggcggatg
     5101 atgttgatgt ggcgatattt tatggtcggg gcaactggcc ggggctacgg gtggaaaaac
     5161 tgtacgccga atatttattg ccggtgtgtt cgccgctact gctgacaggc gaaaaaccct
     5221 tgaagacccc ggaagatctg gctaaacata cgttattaca tgatgcgtca cgccgtgact
     5281 ggcagacata tacccgacag ttggggttaa atcatatcaa cgttcagcaa gggccaattt
     5341 ttagtcatag cgccatggtg ctgcaagcgg ctattcacgg gcagggagtg gcgttggcaa
     5401 ataacgtgat ggcgcaatct gaaatcgagg ccggacgtct tgtttgcccg tttaatgatg
     5461 ttctggtcag taaaaacgct ttttatctgg tttgtcatga cagccaggca gaactgggta
     5521 aaatagccgc ctttcgccaa tggatcctgg cgaaagccgc tgctgaacaa gaaaaattcc
     5581 gctttcgtta tgaacaataa tttacgtagg gtacgaccat gaccagccgt ttcatgctga
     5641 ttttcgccgc cattagcggc ttcatttttg tggctctggg cgcttttggc gcgcatgtgt
     5701 taagtaaaac catgggggcc gttgagatgg gctggatcca gaccggcctc gaataccagg
     5761 cgtttcatac gctggcgatc ttaggtctgg cggtggcaat gcagcgtcgc atcagtatct
     5821 ggttttactg gagtagcgtt ttcctcgcgt taggcacggt gttgttcagc ggcagccttt
     5881 attgcctggc gctgtcccat ctgcgtttgt gggcgtttgt cactccggtt ggcggcgtga
     5941 gcttcctcgc gggctgggcg ttaatgttag ttggtgctat ccgtttaaag cgcaagggcg
     6001 taagtcatga ataaggttgt attgctgtgc cgtccgggct ttgaaaaaga gtgcgccgca
     6061 gaaattaccg ataaagccgg ccagcgggaa attttcggtt ttgcccgcgt gaaagagaat
     6121 gcgggttatg tcatttatga atgttatcaa cctgatgatg gcgataagtt aatccgtgag
     6181 ctgccgttca gttcattaat ttttgcccgc cagtggtttg tggtggggga actcctgcag
     6241 catttgccgc cagaagatcg tattaccccc attgtcggca tgttacaggg cgtagtagag
     6301 aagggcggtg aactgcgtgt tgaagttgcc gataccaacg aaagcaaaga gttactgaaa
     6361 ttctgccgta aatttactgt tccgctacgc gctgccttgc gcgatgccgg ggtgctggcg
     6421 aactatgaaa cgccgaagcg tccggttgtg catgtattct ttattgcacc aggctgctgc
     6481 tataccggtt actcatacag caacaataat tcgccgttct atatgggcat tccgcgcctg
     6541 aaatttccgg cagatgcgcc gagtcgttcc acgctcaaac tggaagaggc atttcatgtg
     6601 tttattcctg cggatgagtg ggatgaacgc ctggcgaacg ggatgtgggc ggtggattta
     6661 ggcgcttgcc ctggcggctg gacctaccaa ctggtgaagc gcaacatgtg ggtttattcc
     6721 gtcgacaacg gcccgatggc gcaaagtctg atggataccg gacaggtgac gtggctgcgg
     6781 gaagacggtt tcaaattccg tccgacgcgc agcaatatct cctggatggt atgcgatatg
     6841 gttgaaaaac cggcgaaagt tgcggcattg atggcgcagt ggctggttaa tggctggtgc
     6901 cgtgaaacca ttttcaacct caaactgccg atgaaaaaac gctacgaaga agtgtcacac
     6961 aatctggcgt atattcaggc acagcttgat gaacatggca taaatgctca gattcaggcg
     7021 cggcagttgt atcacgatcg cgaagaagtg acggtgcacg tccgccgcat ctgggctgcg
     7081 gtgggtggtc gtcgcgacga gcgataacag gcaaggccgg ggcgtcgtct ccggcctgct
     7141 accctttccg tcaggctgtt accaaagaag ttgcaacctg gcgctcatca gaaataatgt
     7201 gcgttacctc atccagatgc ccgatgcggg cttcccctga acgattaaat ttacttttat
     7261 caatcaataa caacgattgc gcggcacgtt ttaatagcat cgatttgtaa tcagcgttga
     7321 tcgcattgga gtcccacagt gcgccgctgc tatcgatccc ttcacaagaa aaaataaaca
     7381 gatcgatttc cagcgattta agttgggaaa tcagcgaggg attgacgtaa cagccatatt
     7441 tgcgctcaag tgtgccgccg gaactgatca gttgaatgcg ttcgcgttta ccgagttcat
     7501 ggcaaatcgg atggctattg gtgaagacct gaatgttgat gtcaggcaac tggcgtgcca
     7561 gataccagca agttgaactg gcgtctaagg ctatcaccat cccttcttca atccacgcga
     7621 gcgcctcgcg cgcgatatct gctttatgcg cataatggct tttcagcctg atgtgaaagg
     7681 gatcgccact gtcttgattt tgacggtgga tatatttagc gcgtccatga ttgcgcagaa
     7741 ttttaccctg cgtctgtaat tcattgagat cgcgacgaat ggtttcttta ctgaccttta
     7801 gctgttcaga gagagcttcc gtggtcaggc tggtatggtt cagcagcagg tcgactatcg
     7861 cttgctggcg tgccgctttc atatttttca ccccatcatt cgataaccgc ccgcgtcatg
     7921 cgggcgtact ggtatgagat tacggtgtta cccctttttt taaaagaata ttcccgtact
     7981 tcgctcgttc gcctgtgata acgatcgcaa aggctttttg cgcccgttca taaaacgcaa
     8041 aacgattgat gcggatgatg tcaggacacg gggcttgtag tgaaagcgca ttacggtaac
     8101 gtcgttctac ttcaggatcg agagtgtcac cttctaccgc cgccatcatc accagcggcg
     8161 gtgcataact gtccagttca aataacggga taatcgcctg gagcaagtcg ctcaccaaca
     8221 ggccatcagc gcggatcacc tgcggtccca tcgaatgggc gggaaagtga gcatcggaaa
     8281 aaataatttc atctccatgt cccatctctg ccagcacttt caatagttcg ggagaaatta
     8341 acggcgaaat tgttttcagc atttcacact tcctctataa attcaggttc agtttgcggg
     8401 tagaaataac ggtactgata atgaatctgt gcgcgggctt cttccgggct gttaaattcc
     8461 cctacgccat accaaccgaa cagcgcagct cctgcgacgg tcgtttcggc gtcgtcgaga
     8521 acttttaccg gaatatcaag catattggct ttaatctgat tccacaatgt gttgcgactt
     8581 cctccaccga ctaacaataa ttcagaggcc ttaaagtgcc cgattttttc cagcatctgt
     8641 agattgcgct gtaattgcgc agttaaccct tccagcgccg cgcgatagaa atgcccccgc
     8701 gtggtattaa gcgtcactcc ttgccagcca gcgttctgac acgacaataa atcacactgc
     8761 atttttacgc catccgcgcc aggcgcgatc agacgagctt cttcaatcaa catttgccag
     8821 ggtgtttcag ccgtccagaa cagttttctc acccattcca gcacgccgga tgccagccat
     8881 tgcatacctg ggttatacaa ccctgcctgg ctatccagtt cgcaggtgga accggcgtac
     8941 tgacttaaca gcgaagtatc aacctgggcg ctacggacca ttaaaatttc ccatgtaccg
     9001 gaagagagca cgggttcatt ttggtcggca ccagcgccaa aaagggcgaa ctgggtatcg
     9061 tgacctgcgg aaatcaccgg tatgccaacg ggtaagccga gcattgctgc ggcgctgttc
     9121 tgtagcgtac caatctgttc acccgcttcc accagacgag ggaagagtcg gcgtggaata
     9181 ccggtggctt gtaaaatttg cggactgaaa tcgcgttgct ggatatccag catctggctg
     9241 gttccggcca tcgtgatatc agtagtgaat tcgccggtta aacggtggtt aatcagcgac
     9301 gaaataaaga gccaggcgtg cgcgcgttcc agcagttgtg gatgattttc tttcaaccac
     9361 accaacttat ataacgtatt gaaactaaag gctccgacgc cagaaatagc ctgcaaccgc
     9421 tgtgcggaga tcaactgttc aatattgtcc ataaccgctg ctgttcgcgg acatttccag
     9481 ctaatgatcg gatagagcag attgccttgc ttatctacca gagcgccatc cacaccaaag
     9541 gtggtgacgg cgataccgcg gatgtggcat tcagtcagtt cactattgat ttgccgacag
     9601 caatcagcaa agcgttgcaa aatggcgtct aaagaccact ggtgccaggt gttgttttcc
     9661 atcgcgatat cgctggcatt aggcgttgag gcgcgggcaa caatttttcc ctgccgatta
     9721 accgcgatgg ccctgacatt ggtcgcgcca cagtcgagta ccaggataac ttcttgtttc
     9781 ataatcgctc ctgcaatata gccggataac attgcttatc cggctaacca ctcttgcttg
     9841 ttcaggcccg gtagccggag cgaccgggca tcacatcagg gagtaatgta ttaacgcttg
     9901 tacaacgggc cgtagttctg gcaagcgcgg taatcctgac cttcaatatc catgccgtgc
     9961 gcggcccagg cagaaggacg atatactttg gtctcttcaa cgttgtgcat acataccggg
    10021 atacgcagca tggaggcgag agtgataaag tctgcgccaa cgtggccgat ggtcagaacc
    10081 ccgtggttag cgccccagtt cgccattacc gaatacacat ccgtaaacgg ccctttaccg
    10141 gtgaggcgcg gtgcaaacca ggtggttggc caggttgagt tggtgcgttt gttgaggatg
    10201 tcatgcacat ccttcggcaa ttccacgctc cagccttccg cgatttgcag taccggtccc
    10261 aggcctttga tgatattgac acgggtcatg gtgaacggga cgccgccttc ggtaaggaag
    10321 cgggaagagt aaccgccgcc acggaagtat tcgtggatcg ccgggcacca ttcggtagcg
    10381 gcgaggcaag cgtcagcctc ttgctgagag atttcccagt gcggcttcat cgtcgggtta
    10441 ccttcgctgt cgcgttgttt acaggaaccg tccagcgcag cagaacctga gttgatcaaa
    10501 tggatgatgc cgtgttctgc cagtccatcg agtttatgcc ccgttacacg ctcaattgct
    10561 tctggtgacc agtaggtacg cacatcggca aatacctgag cggtgccggt gagctggtga
    10621 cccattagca ttgccacgcc gttaagactg tcgttttcgg tcgccacgac aaagggttcg
    10681 cgcacgccat tccagtcaaa tgaactgttg aggatcgctt cggcggtgtc accattggga
    10741 tattgatcgg tccagtgacg ttgcccctgg aagcccgcag cgatggcgtt gtagccaagt
    10801 gattcttcca cgcgaccaat atcggccagt ttgctgttgc cttgcatcat gtcgcggata
    10861 cacatcgcca tcagtaaact ttcgcgcaga actgcgcggc tttgctcggc attgcgttga
    10921 tactgtttgt tattttcatc ttcgccatag cggaagtttt tatcagccca ggtcagtgcc
    10981 atttccagtt cggcttcgtc gtaaatcttc tgatcgatac ggcgacgcag ttcggtcata
    11041 tccaccgcct ggactttcat tcccagccag gattcaaaga agttgtgatc aacaatagaa
    11101 ccggcgatac ccatcgaaac gccgcccagc gacagatagc ttttaccttt catgctggcg
    11161 acggccaaac cggcgcgggc aaagcgcagc agtttttctt caacatcggc aggaatcgat
    11221 gtgtcatcgg catcctgaac gtcatgaccg taaatggaga atgccgggat gcctttctgg
    11281 ctgtgagctg ccagagccgc tgccaggtaa acagcgccgg gacgttcagt gccgttaaag
    11341 ccccaaatgg ctttcgggcg ggttggatcc atgtcgatgg tttcactgcc atagcaccag
    11401 caaggcgtta ccgtaatggt gaggcctaca ttctggctgc tgaatttttc ttcgcaagca
    11461 gcggcttcag ccatacccgc gatacaggta tcggaaatga cacactcgac ggcagctccg
    11521 caggcatggc gcagtttctc ggtcagcagt gcggccgtag ctttcgccat attcattgtt
    11581 tgttcttcaa gcgactcacg aacacccatg cgacgaccgt caataaccgg gcggatacca
    11641 attttcggta agctgatttt tttcattaca gattcctcac tttattcgga aaatatgttc
    11701 agttagttgc cgtttgagaa cggaaacggg caaagataaa gatgaccgcg aagcagagtg
    11761 cggggatcag ttcagcagtg gggatgttgc ccgccgcgtc actgacaaaa cccatgaccg
    11821 gagtgacaat accgccgcca atgatggtca taacgatgaa ggacgaaccg tatttggtgt
    11881 cctggccgag attcttaatg cccagcgaga agattgttgg gtactgaatc gacataaagg
    11941 cgctgcataa agtcagggct attaagccca catgaccgcc tgcgaaggct gagatcaggc
    12001 acagtgccat agcgattaat gcgtaggcgg ccaggacttt gtgtggtgcg aagcgactga
    12061 tgagccaggt accggtgaaa cgaccaataa agaagcacac catggttccg gttaaatagt
    12121 tagcggcaaa gcctgcagtc atacctggaa tttcttctac agcgtagcga atcaaatagc
    12181 tccagcaggc cgtttgtgcg ccgacatagc agaattgcgc taataccgcc cagcgccagt
    12241 gacgaatacg cgccagacga gaaagcgatg cggagaacga tccttgtttg gcgtcactgt
    12301 gattatcact ctgcaatgcc gggaatttcg tcagcatgat cagcagggcg accagtaaca
    12361 cgatagccac gatgatcata taaggtgtct gtaccgataa taccaggctg tgtttatacg
    12421 cactcaattg ctctggagac attttatcga gaacgtcttg cgattgatgt ggcacgttag
    12481 acaaaataag actttgccca aagacaaccg cgataattgc gccaaacgag ttaaatgttt
    12541 gcgcaagatt taagcggaag tgaccactgc tttccggccc taataccgta acaaaagggt
    12601 ttgcggcagt ttccagacaa cctaatccgg ctgcaataat aaataggcca actaaaaata
    12661 aggtgtagtt cattatttct gcagcgggcc agaataatgc agcgcccaag gcatataaaa
    12721 ataatccggt aataatccct gctttataac tgagtttttt catcaatatc ccagcaggga
    12781 ttgggataat gaaataacca aagtaaaagg ccgattggat caggccagcc tggaaatttg
    12841 tcagcgtaaa agcctgctgg aattgaggta ataaaatgtc gttaaggtta ttggctaccg
    12901 cccaaagaaa aaacagtgag cacagcagcg cgaatggaat aatgtaactt ctgctttgcc
    12961 ctgcatcttt atctaccgca cggtaactct gcgtttgtat tgatgtgttt cccatagcat
    13021 cctcttaggt tcagaagctt aagcacgtaa tcggttatgt tttcaaaagt gcaaaaccag
    13081 cgagttcggg cttcatatca gtgaatattt gcctgtctga aatgaactca tggattcatt
    13141 ttcaaataaa aaacgctctg taatatgacg gcggtcacac ttattagctg gaaggtgact
    13201 ttatgtgact accatcactt taataatcga aatttcatgc ccgtttcaga aaataaatca
    13261 taaaaaacac aaaccagatg accgtattaa ttgtttatgc cgaaaacggg cattcctggc
    13321 ttgggtgaat agaaaaatga ccgttttgat aaatattttt aagagcggtc atttttaatg
    13381 gggttataaa aagagaactt gcagttattt actgtgatta gttgaaccag gtcacaaaat
    13441 ataagcttgt gaatttcttt ctggttaatc aggaagaaga tggtgggtaa ttaaacggct
    13501 aattcaatag tgtgaaagga acaacattat tgccctgttt tgaatcagag agaggtagct
    13561 aatggaacga aataaacttg ctcgtcagat tattgacact tgcctggaaa tgacccgcct
    13621 gggactgaac caggggacag cggggaacgt cagtgtacgt tatcaggatg ggatgctgat
    13681 tacgcctaca ggcattccat atgaaaaact gacggagtcg catattgtct ttattgatgg
    13741 caacggtaaa catgaggaag gaaagctccc ctcaagcgaa tggcgtttcc atatggcagc
    13801 ctatcaaagc agaccggatg ccaacgcggt tgttcacaat catgccgttc attgcacggc
    13861 agtttccatt cttaaccgac cgatccccgc tattcactac atgattgcgg cggcaggtgg
    13921 taattctatt ccttgcgcgc cttatgcgac ctttggaaca cgcgaacttt ctgaacatgt
    13981 tgcgctggct ctcaaaaatc gtaaggcaac tttgttacaa catcatgggc ttatcgcttg
    14041 tgaggtgaat ctggaaaaag cgttatggct ggcgcatgaa gttgaagtgc tggcgcaact
    14101 ttacctgacg accctggcga ttacggaccc ggtgccagtg ctgagcgatg aagagattgc
    14161 cgtagtgctg gagaaattca aaacctatgg gttacggatt gaagagtaat ttcgtaaagc
    14221 aacaaggaga aggatgatgg ctaacagaat gattctgaac gaaacggcat ggtttggtcg
    14281 gggtgctgtt ggggctttaa ccgatgaggt gaaacgccgt ggttatcaga aggcgctgat
    14341 cgtcaccgat aaaacgctgg tgcaatgcgg cgtggtggcg aaagtgaccg ataagatgga
    14401 tgctgcaggg ctggcatggg cgatttacga cggcgtagtg cccaacccaa caattactgt
    14461 cgtcaaagaa gggctcggtg tattccagaa tagcggcgcg gattacctga tcgctattgg
    14521 tggtggttct ccacaggata cttgtaaagc gattggcatt atcagcaaca acccggagtt
    14581 tgccgatgtg cgtagcctgg aaggactttc cccgaccaat aaacctagtg taccgattct
    14641 ggcaatcccc accacagcag gcactgcggc agaagtgacc attaactacg tgatcactga
    14701 cgaagaaaaa cggcgcaagt ttgtttgcgt tgatccgcat gatatcccgc aggtggcgtt
    14761 tattgacgct gacatgatgg atggtatgcc tccagcgctg aaagctgcga cgggtgtcga
    14821 tgcgctcact catgctattg aggggtatat tacccgtggc gcgtgggcgc taaccgatgc
    14881 actgcacatt aaagcgattg aaatcattgc tggggcgctg cgaggatcgg ttgctggtga
    14941 taaggatgcc ggagaagaaa tggcgctcgg gcagtatgtt gcgggtatgg gcttctcgaa
    15001 tgttgggtta gggttggtgc atggtatggc gcatccactg ggcgcgtttt acaacactcc
    15061 acacggtgtt gcgaacgcca tcctgttacc gcatgtcatg cgctataacg ctgactttac
    15121 cggtgagaag taccgcgata tcgcgcgcgt tatgggcgtg aaagtggaag gtatgagcct
    15181 ggaagaggcg cgtaatgccg ctgttgaagc ggtgtttgct ctcaaccgtg atgtcggtat
    15241 tccgccacat ttgcgtgatg ttggtgtacg caaggaagac attccggcac tggctcaggc
    15301 ggcactggat gatgtttgta ccggtggcaa cccgcgtgaa gcaacgcttg aggatattgt
    15361 agagctttac cataccgcct ggtaaatgcg ctgatgtgat aatgccggat acgacgtttg
    15421 cggcgtatcc ggctcgccgt taccgtacca accgcaattg ctttaaattg ccgtcgatat
    15481 gcaaatcggt ttgtaagcgg gcaatatcgc ggcacagaaa cgccatctct ttatgggttt
    15541 ctaatttttt gcgccacttt tcggcaaccg catccagatt ctcatatatc ccttccagac
    15601 tctgaaactc gaccagcagc tgcgtggcgc tttttggtcc gattcccgca acacccggta
    15661 cctttgaact gctgatcccc gcaagtcccc agtaatcggg caactgctgc ggttgaacgc
    15721 caaattcttt atcgataaat ggcgcatcca gccaacgttt ctggaagtaa tcacgaatac
    15781 gtaatgtcgg tgaaagtaac tgacagtagc ctttatctgt cgaaacaatc gttgcctgat
    15841 gcccggcctg tgtcactttg accgccagcg tggcggctaa gtcatcggct tcgttgccgc
    15901 tggctgacca gcacgggacg ccgcgttgct caaaggcggc gcgtaatgca ggcatctcgt
    15961 cgtgcaactc ttccggcatt ggcggtcgac ccgctttgta atctggtaaa cgctgatgac
    16021 gccagccgct actgcggttt tcatcatcaa aaacggcgac agcgtgggtt ggctggctgt
    16081 gcataatgag ctgatcgagc gcgtgctggc aggtttcgac acagggcgac ccctgaacgg
    16141 catgaatgcg acgaataaga ttcagtgcat cgacaataag caaatgaaca gccacgataa
    16201 cccccttaaa gatcaggcga taagggtaac attcgatgcc ggaacaggct acggctggat
    16261 gagaaatcgg gaagaggcct cgcaaaacga ggcctctgga gagcgattaa tcgcaggcaa
    16321 cgatcttcat tgccaggccg ccgcgagagg tttcgcggta cttggcgttc atatctttac
    16381 ctgtttcgta catggtttcg ataactttat cgaggcagac gcgcggctcg ctggtacggc
    16441 gtagcgccat acgtgcggcg ttcaccgctt ttactgccgc aatggcgtta cgctcgatgc
    16501 atggcacctg gacctgtccg gcgaccgggt cacacgtcag accgaggttg tgctccatgg
    16561 cgatttccgc cgcgatgcac acctgcgccg ggcttgcgcc taacagttct gccagaccag
    16621 ccgccgccat tgagcacgcc acaccaactt caccctggca gcccacttcg gcaccagaaa
    16681 ttgatgcgtt catcttataa agagaaccaa tggcgctggc taccagcagg taacgagcca
    16741 gtgagttagc gttcacttcg cggataaact tatcgtagta cgccagcaca gccgggataa
    16801 tcccgcacgc accgttagtc ggcgcagtca ccacgcgacc gccagcggcg ttctcttcgt
    16861 tcactgccag tgcaaacatg ttgatccagt caactaccgc catcgggtca gtggtggttt
    16921 tatcctggct gaccagcatc cggcgtagtg ccgcagcacg gcgtggaacg cgcagtttgc
    16981 caggcaacac gccttcggtg gaaataccgc gctcaatacc gccgcgcatg acttcccaga
    17041 cgttcgccag gtgctgttcc agctcttctt tgctgtgcag cgccagctcg tttttcatca
    17101 tcaggccaga gagtgacagc ccggtttcct gacaatgttt ttgcagatcg gctgctgaac
    17161 tgtacggata aggaacttca accggtgcgc tatcctgctg gccaaaatgc tcttcatcaa
    17221 cgataaagcc accgccaata gagtagtaag tctggctgta aacgacttta tcgcccgcca
    17281 gcgcggtaat gcgcataccg ttttcatgca gagaaaggtt gtcggcatgg aagttcatgc
    17341 actgatcaac cgggaactcc acttcatgct gaccatttgc cagcatcagg cgaccatgag
    17401 tattcacatc ctgaataaaa ccagggatgg aatcgatatc cacggtatct ggcaggttac
    17461 ccgccaggcc cataataatg gcgatatcag tgtggtggcc tttaccagtc agagagagcg
    17521 agccgtacac gtcaaccacc acgcgggtca cgtctttaag caggttacgg gcaatcagat
    17581 cgtcggtaaa ttgtttaccc gctttcattg gtccaacggt atgagaactg gaagggccaa
    17641 tgccgatttt gaaaatatcg aatacgctaa tcataggaaa tacatcgcgt taaaacggag
    17701 gaagcgccgc ccgaaagcgg cgcgaaagga cttagctgaa cagagagtag aagattgcgg
    17761 agattgcaat cagacccatc acgacaacga atacgttgct gatgtgaccg ctgtacttac
    17821 gcattgccgg tactttctga attgcgtaca tcggcatcag gaacaggatc atcgcgatga
    17881 ttgggccgcc cagggtttca atcataccca ggatgctcgg gttcagggtg gcaacaatcc
    17941 aggtcgttac cagcatgaac agcgcagtga tacggttcag cttgttgatt tcgatagact
    18001 tacctttacc acgcagagat ttaatcacca taccgttgaa gccttcacgt gcgcccaagt
    18061 agtggccgag gaaggatttg gtgatagcga taatcgcgat aatcggagcc atccacgcga
    18121 taaccggtgc gttaaagtgg ttagccaggt aagacagaat cgagatgttc tgctctttag
    18181 ccgcagccag gtctgccgga gtcaggctca gtacgcagct gaagacgaag aacattacgg
    18241 tcagcaccat catgatgtgt gcgaatgcca ggatcttcga gcatttctgt tctgccatat
    18301 cgccgtactc ttcacgcttc gcaacggcga aagaagagat gatcggagag tggttgaacg
    18361 agaacaccat taccggaatt gccagccaca gggtcatcca cagaccgttt ccggttgcag
    18421 atgcagtgtc cagagacagc gtttccagtg ctgcgccgtt ccactgcggg atcaggtaca
    18481 gagccagcag catcagtacg ccaacaaacg ggaataccag aatactcatc gctttaacga
    18541 tcatctgctc accaaagcga acgatggtca tcatacccac gatcaggatc agcgacagaa
    18601 tcgcacgcgg cggtggcgtc atacccagct ggtgagacat gaagctttca actgtattgg
    18661 tgattgccac gctataaacc agcaggatcg ggtagatagc gaagaagtag agcagggtaa
    18721 tcagtttacc tgcgccaata ccaaagtgtt cttctacaac ctcggtgatg tcttcgcccg
    18781 ggtttttacc agacagtacg aagcgagtca ggccgcggtg agcaaaaaac gtcatcggga
    18841 acgcaaggat agccatgatg atcagcggga tcataccgcc aacaccggcg ttgattggca
    18901 ggaacagcac gcccgcgccg attgccgtgc cgtaaaggcc cagcatccac atggtgtctg
    18961 tcttgcgcca ggcactacga gagtctttcg acgcaatcgt gctggtttga gtcgtttcca
    19021 tctatttctc ctggaggaat gcaaataatt cagcttttag ccagttcagc ccgggaaggt
    19081 gccagaccgt aaaaatgaaa tctgtctatc ggcgatggct taatcattta ccgccgtaat
    19141 aatttgaggg cggaaagata cacacaagtt atgtatctat cagtgatctt gatctcaaat
    19201 acaataatct acttggaaga tagattatcc caacgcttta ctctgtaaat caaaagtaaa
    19261 agagaattta agggcaatat ttgcccatta aacgacatct gacttcttcc ggggcgcgca
    19321 atatatgtag gttgaactga aaaaatcaat acttttagtt aatcatggcg ataatttctt
    19381 tttcatagcg ataaaatacg gtttatgaaa atagggaaac gtttgcgttg caacataaat
    19441 tgtatgacat attaactgtg atgatatttg tcgcgtgagg gtggtaagaa acattcatat
    19501 aacaaatgaa atgagttgat gagtaagtga ccaaaaacga gaaaacccgg caacaaacgt
    19561 caccggggga gcggagatta cgtgcagatt tcgtagcaag ggatgtaggc tgagcctggc
    19621 aacttcatac gatgctgggc aacaaaaccc tgtagcaggt cgtccatacg acgcataatc
    19681 tctttatcgc cgttgatttt gtaaggacca aactcttcaa tggcgcgaat accgacttct
    19741 tttacgttac ccgccacaat accggagaac gcacggcgca ggtcggcagc cagcacttca
    19801 acaggttgat ccgggtaaag cttcagatta gccatattct cgtgagacgg ctcaaacggc
    19861 atttgcaaat ctggtgcaat gcgcattgac cagttaaagc tgtaagcgtc accagtgtcg
    19921 cggcgatttt ctttcaccag cggcatcgat tttttcatct gacgagcgac ttcagcggcg
    19981 tcatcaatga tgatgcggta atggcggcgc gcgttttcgc ccagcgtatg tacgacaaac
    20041 tcgtccagta cgcggaagta gtcggcgctc tctttcgggc cggtgaggat caatggtaaa
    20101 acctgatctt tgttggccgg gttcattaaa attcccagca aatacagcaa ctcttctgcc
    20161 gtacccacac cgccagggaa gataatgatg ccgtgagcga tacggacaaa cgcttccaga
    20221 cgtttttcga tatccggcat gatgatcaat tcgttgacca gcgggttagg cggttcagcg
    20281 gcgataatcg acggctctgt cataccaata aaacgactgt ctttgtaacg ctgctgcgcg
    20341 tgtccgaccg cagcaccttt catcggcgct tccatcgctc ccggaccaca gccggtgcag
    20401 atattcagct cacgcaggcc cagctggttt ccgacgcgac gggcatacaa atactcattt
    20461 tcgttaattg agtgaccgcc ccagcagacc accatatttg gcgcttcacc cacatgcagc
    20521 gcgcgagcgt tacgcaagat ggaaaagacc aggttagtga tgtggacgga gttgtcgaga
    20581 ttcaggttgg gaaaacgaac ggtgttatgg atttgcccgt aaacgaagag aatgtcacgc
    20641 aggaccgcga acaagttggc ctgcaaagcg cgaataattc gcccatcgac aaaagcctct
    20701 tccgggggat taatcagttc cagctttacg ccgcgttcac ggcgcaagac gttaatatcg
    20761 aaattttcaa aacgagacag caattctttg ctgttatcgg tcaaactacc ggagttcagt
    20821 acggcaagtg aacagttgcg aaacagttga tagaggtcgc tgctggcggt gcgtttaagc
    20881 atatccactt ccagctgcga caacatatcc atggagccaa gcgggctaat atgtgtaatc
    20941 aagaaaaact ccttatggga cgaatatcgt cattcccttt gattacaata gccctgcctg
    21001 atgacctttc acaaccttct gcgtgaatcc agcacgcaaa attgagaaaa aatttattgc
    21061 cgaaccagtc ttgtggtcga tgggacaaaa tcgctattac tgcgccacgg gttaatgtcc
    21121 agaccgccac gacgggtata acgtgcgtaa acgctcaatt tttctggctg gcagaagcgt
    21181 aacaggtcat taaagatgcg ttccacgcac tgttcgtgga actcgttgtg atgacggaat
    21241 gagaccaggt aacgcagcag tttttctctg tcaatttggc gtccacgata ctgaatttgg
    21301 atcgaacccc aatctggttg atgggtgatc aggcagtttg atttcagcag gtggctgaca
    21361 agcgtctctt ccactacttt ttcaccgctg gtggcattct ccagatagtc agtagtgaat
    21421 tcatagttat cgatagtgat atcctggtca tcaatgcagg tgccattgaa atgacctatc
    21481 ggctggcctt ccagttcatc aagacgatat aacgccacgc taaccttacc ctgggcgcaa
    21541 gtgcttaagt cgcgctccag cgtctggcgc acctcatccc agttattaaa acgcgtctgg
    21601 ttaaaactgt tgagatagag cttaaaactc ttcgactcaa tcagatttac gctggtgtaa
    21661 tcaagttcaa catgaccgac agcgacctgc ggcaaacctt tcgcattcag ccaggaaagt
    21721 tcatacagcg tccagatatc cgtaccgtga aaaggcaggt tatccgcttt cagacccagc
    21781 gggtcgcgat tcaggctgcg tggaacgcct tgcagtaggc tggcgtcata ggtatcccgg
    21841 taatcggttg attttccaag agtcaggccc gcaagtgcct gatggtttgc ataagaagac
    21901 atgtttcacc gtcataaacc agataaacta aacgacaagt gtatctggtt taagtgaaga
    21961 gagaaattag tggacgattt gaccgcacaa gccctgaaag attttactgc acgctactgc
    22021 gatgcgtggc atgaagagca taaaagctgg ccgttaagtg aggaactgta cggtgttcct
    22081 tcaccgtgca ttatttcaac taccgaagat gccgtatact ggcaacccca gccgtttacc
    22141 ggggaacaaa atgtaaacgc ggttgaacgc gcttttgata ttgtgataca acccacaatt
    22201 catacctttt atacgactca gtttgccggg gatatgcacg cgcagtttgg cgatatcaaa
    22261 ctgacattgc tgcaaacctg gagtgaagac gacttccgtc gggtgcagga aaatctgatt
    22321 ggtcatctgg tgactcagaa aaggctaaaa ctgccgccca cgctatttat cgcgacgctg
    22381 gaagaagagt tagaggtcat ctcggtgtgt aatctctccg gtgaagtgtg caaagagacg
    22441 ctgggaacac gtaaacggac gcatttagcc tcaaatcttg cggaattcct taatcaactt
    22501 aagcctcttc tgtaatccaa ataccccatc tggttgtgag agatctctta cagactctgt
    22561 aggagatcgc caggaaataa gcgaatactt aaaaagataa gaatcgttat tttcatttaa
    22621 aatcaaaatg ttgattgtta attcttaact ttcatatgaa attttcctta aggcattgtc
    22681 tgtaagcgcc ttgtaagact tcgcgaaaaa gacgattcta tcttcgtcga cagggagtca
    22741 gacaacgaag tgaacatcag gatgatgaca cttctgcagg acacaccagg atggtgtttc
    22801 agggaaaggc ttctggatga agcgaagagg atgacgcagg acgcgttaaa ggacacctcc
    22861 aggatggaga atgagaaccg gtcaggatga ttcggtgggt caggaaggcc agggacactt
    22921 caggatgaag tatcacatcg gggtggtgtg agcaggaagc aatagttcag gatgaacgat
    22981 tggccgcaag gccagaggaa aagttgtcaa ggatgagcag ggagcaacaa aagtagctgg
    23041 aatgctgcga aacgaaccgg gagcgctgtg aatacagtgc tccctttttt tattcctgct
    23101 atccttcgcg gcagtttttc ttattgaggt tgctttatga ccactcatga ccgcgttcgt
    23161 ctccagttgc aggcgcttga agcgttactg cgtgaacatc agcactggcg aaacgatgaa
    23221 ccccagccgc atcaatttaa tagtacccaa ccgttcttta tggacaccat ggaaccactg
    23281 gagtggttgc agtgggtgct gatcccgcga atgcacgatt tgttagataa caaacagccg
    23341 ttaccgggcg cttttgccgt tgcaccctat tatgaaatgg cgctggcgac ggaccatccg
    23401 caacgcgcgc ttattctggc tgagctggaa aaactggatg ctctttttgc ggatgatgcg
    23461 agctaatgct ggaaatactc tatcaggatg aatggctggt tgcggtaaat aaaccctccg
    23521 gctggctggt tcaccgcagc tggctggatc gcgacgagaa agtagtggtc atgcaaaccg
    23581 tgcgtgatca gattggccag cacgttttta ccgctcatcg tctggaccga cccacttctg
    23641 gtgtgttgtt gatgggatta tccagcgagg ccggacggct gctggcacaa cagtttgaac
    23701 agcaccaaat ccagaaacgt taccatgcga ttgtgcgcgg ctggttgatg gaagaagcag
    23761 ttctggatta tccactggtg gaagaactgg acaaaatcgc tgataaattt gcccgcgaag
    23821 ataaaggctc gcagccagca gtgacgcatt atcgcggtct ggcgaccgta gaaatgccgg
    23881 tagcgaccgg acgttacccg accacgcgct acggcctggt ggaactggag ccgaaaaccg
    23941 gacgcaaaca tcagcttcgc cgacatcttg cccatttgcg tcatccgatt attggcgata
    24001 gcaaacatgg cgatttacgc cagaatcgca gcggtgctga gcattttggc ctccagcggt
    24061 taatgctaca tgccagtcag ttgtcattga cgcatccttt tactggcgag ccgctgacta
    24121 ttcacgcggg cctggacgac acctggatgc aggcgttatc acaatttggc tggcgcggtc
    24181 tgcttcctga aaatgaaagg gttgagttca gtgcgccatc gggtcaggat ggagagataa
    24241 gttcctaatc cagggagaaa acggtatggc ggaaattggt atttttgtcg gcaccatgta
    24301 cggaaattcg ctgttagtgg cggaagaagc agaagcaatt ctgaccgcac agggccacaa
    24361 agcaacggta tttgaagatc ctgaattaag cgactggctg ccctatcagg ataagtatgt
    24421 tctggtggtt acgtccacga ccgggcaggg cgaccttcct gatagcattg tgccgctctt
    24481 tcagggaatc aaagatagtc tgggcttcca gccgaatctg cgttatggcg tgattgcgct
    24541 cggtgacagc agttatgtaa atttctgcaa tggcggcaaa cagttcgatg ccttgttgca
    24601 ggaacagagc gctcagcggg ttggcgaaat gctgttgatt gacgccagtg agaacccgga
    24661 accggaaacg gaatcaaatc cgtgggtcga acagtggggc acgctgttgt cctgatatgt
    24721 tcagcgagcg gtaaatgtcg ttttagcggt gctgaatcga atattttcag gcaaatgcca
    24781 gtaaaaactg cttcatagcg cggattttta ctggcgtttg cctggagtca agcgatccat
    24841 ttcatactct tctttatttc ttcgttttaa cccttccttt cttgttcttg ttttcatttc
    24901 cgtgaagtgg attccaccgt ccagggctaa tgccaaaatc gggcctcatt gaacgcatta
    24961 atgttgtgtt gttgcacggt gagccgctat ggcgcgcttt ttatactgct attgccagat
    25021 ataaacacgc gccgtattcg gcgaacgacc tataaaaacg gcaaaaaaca ccctacgtca
    25081 cctctgattt cctggctatg tcgcagtcca gagtgagcgt ggctaacgcg aattttcagg
    25141 agtgcaacaa tgagttcttt aagtcaggct gcgagcagtg tggaaaaacg cacgaatgct
    25201 cgttactgga tagtggtgat gttgtttatc gtcacatcct tcaactacgg cgaccgcgct
    25261 acgctttcta ttgccggttc ggaaatggcc aaagatatcg gccttgatcc cgtgggaatg
    25321 ggctatgtgt tctctgcctt ctcatgggct tatgttatcg ggcagatccc tggtggttgg
    25381 ttgctggatc gttttggttc aaaacgcgtc tacttctggt cgatctttat ctggtcgatg
    25441 tttaccttgc tgcaaggctt cgtcgatatc tttagtggat tcggcattat cgttgccctg
    25501 tttactctgc gcttcctggt cgggcttgct gaagcgccat ctttccccgg caacagtcgc
    25561 attgttgcgg cctggtttcc ggcgcaggaa aggggaacgg cggtgtcgat ttttaactcc
    25621 gctcaatact tcgcaacggt gatcttcgcg ccgattatgg gctggctgac gcatgaagtg
    25681 ggctggtcac acgtcttctt ctttatgggc ggtctgggga ttgtcatcag ctttatctgg
    25741 ttgaaagtca tccacgagcc aaatcaacat ccgggggtaa atcagaaaga gctggagtac
    25801 atcgccgcgg gtggcgcgct gatcaatatg gatcagcaaa acaccaaagt taaagtgccg
    25861 ttcagcgtga agtgggggca gatcaaacaa ctgctcgggt cacggatgat gatcggcgtt
    25921 tatatcggtc agtactgtat caacgccctg acttactttt tcattacctg gtttccggtt
    25981 tatctggtgc aggcgcgtgg gatgtcgatt ctgaaagcgg gctttgtggc ttccgttccg
    26041 gcggtttgcg gttttatcgg cggtgtgctg ggtgggatta tttccgactg gctgatgcgc
    26101 cgcacgggat cgctgaacat tgcgcgtaaa acaccgatcg taatgggcat gttgctgtcg
    26161 atggtgatgg tgttctgcaa ctacgtcaac gttgagtgga tgatcatcgg ctttatggcg
    26221 ctggccttct tcggtaaggg catcggggcg ctgggttggg cagtaatggc agataccgcg
    26281 ccaaaagaga tcagcggtct ttccggtggc ctgttcaaca tgttcggtaa catttctggc
    26341 atcgtcacgc caatcgcaat tggttatatc gttggcacga ctggctcctt taatggggcg
    26401 ctgatttatg ttggtgttca tgccttaatc gcggtactga gctacctggt gctggtgggc
    26461 gatatcaagc gtatcgagct gaaacctgtc gcggggcaat aagatgacga cacaatccag
    26521 tcctgttatt actgatatga aagtcattcc ggtcgccggg cacgatagca tgttgcttaa
    26581 tattggtggg gcacataacg catatttcac ccgcaatatt gtggtactca ccgataacgc
    26641 cgggcatacc ggcattggtg aagcgccggg cggagaggtg atttatcaaa cactggtcga
    26701 tgctattccg atggttctgg gccaggaagt tgcgcgcctg aataaagtgg ttcagcaggt
    26761 gcataaaggt aatcaggcag ccgattttga taccttcggc aaaggtgcct ggacttttga
    26821 attgcgcgtt aatgccgtgg cggcgctgga agccgccttg cttgacctgc taggtaaggc
    26881 gctgaatgtt ccggtctgcg aactgttggg gccaggcaag caacgcgaga ctattaccgt
    26941 cctcggttat ctgttttata tcggtgatcg gaccaaaacc gatcttcctt atctggaaaa
    27001 tacgccgggc aaccatgagt ggtatcagtt gcgccatcag aaagcgatga acagcgaagc
    27061 cgttgtgcgt ctggcggaag cctcacagga tcgctatggc tttaaagatt tcaaacttaa
    27121 gggcggggtg ttacctggcg agcaagaaat cgacactgtt cgtgcattga agaaacgctt
    27181 ccctgatgcg cggattaccg ttgatcccaa cggtgcctgg ctacttgatg aagccatttc
    27241 tctatgcaaa gggctgaatg atgttcttac ctatgccgaa gatccatgcg gcgcagaaca
    27301 gggtttctcc ggtcgtgaag ttatggcgga gtttcggcgg gcgaccggct tgcccgtcgc
    27361 gactaacatg atcgccacca actggcgcga aatgggtcat gcggtgatgc tcaatgcggt
    27421 agatattcca cttgccgatc cgcacttctg gacgctttcc ggtgcagtcc gtgtggcgca
    27481 gctttgcgac gactgggggc tgacctgggg ctgccattct aataaccatt tcgatatctc
    27541 tctggcgatg tttacccatg tgggcgcggc ggcaccgggt aatcctaccg ctatcgatac
    27601 ccactggatt tggcaggagg gcgattgtcg cctgaccaaa aatccgctgg agattaaaaa
    27661 cggaaaaatt gccgttcctg atgcgcccgg tctgggcgtg gaactggact gggaacaggt
    27721 acaaaaggca catgaggcct ataaacgtct gcctgtcggt gcgcgtaacg acgcaggtcc
    27781 gatgcagtac ctgatccccg gctggacctt tgaccgtaaa cgtcccgttt tcggccgtca
    27841 ttgattctga aaaaggacat aaatatgagt tctcaattta cgacgcctgt tgttactgaa
    27901 atgcaggtca tcccggtggc gggtcatgac agtatgctga tgaatctgag tggtgcacac
    27961 gcaccgttct ttacgcgtaa tattgtgatt atcaaagata attctggtca cactggcgta
    28021 ggggaaattc ccggcggcga gaaaatccgt aaaacgctgg aagatgcgat tccgctggtg
    28081 gtaggtaaaa cgctgggtga atacaaaaac gttctgacgc tggtgcgtaa tacttttgcc
    28141 gatcgtgatg ctggtgggcg cggtttgcag acatttgatt tgcgtaccac tattcatgta
    28201 gttaccggga tagaagcggc aatgctggat ctgctggggc agcatctggg ggtaaacgtg
    28261 gcatccctgc tgagcgatgg tcaacagcgt agcgaagtcg aaatgctcgg ttatctgttc
    28321 ttcgtcggtg atcgcaaagc cacaccgctg ccgtatcaaa gccagccgga tgactcatgc
    28381 gactggtatc gcctgcgtca tgaagaagcg atgacgccgg atgcggtggt gcgcctggcg
    28441 gaagcggcgt atgaaaaata tggcttcaac gatttcaaac tgaaaggcgg tgtactggcc
    28501 ggggaagaag aggccgagtc tattgtggca ctggcgcaac gcttcccgca ggcgcgtatt
    28561 acgctcgatc ctaacggtgc ctggtcgctg aacgaagcga ttaaaattgg taaatacctg
    28621 aaagggtcgc tggcttatgc agaagatccg tgtggtgcgg agcaaggttt ctccgggcgt
    28681 gaagtgatgg cagagttccg tcgcgcaact ggcctgccga ccgcaaccaa tatgatcgcc
    28741 accgactggc ggcagatggg ccatacgctc tccctgcaat ccgttgatat cccgctggcg
    28801 gatccgcatt tctggacgat gcaaggttcg gtacgtgtgg cgcaaatgtg ccatgaattt
    28861 ggcctgacct ggggttcaca ctcaaacaac cacttcgata tttccctggc gatgtttacc
    28921 catgttgccg ccgctgcacc gggtaaaatt actgctattg atacgcactg gatctggcag
    28981 gaaggtaatc agcgcctgac caaagaaccg tttgagatca aaggcgggct ggtacaggtg
    29041 ccagaaaaac cggggctggg tgtagaaatc gatatggatc aagtgatgaa agcccatgag
    29101 ctgtatcaga aacacgggct tggcgcgcgt gacgatgcga tgggaatgca gtatctgatt
    29161 cctggctgga cgttcgataa caagcgcccg tgcatggtgc gttaagacag aaaaccaaaa
    29221 ccggctctat ctggagccgg cttgagactg atgctaaacg caaaatagcc tgttgtgcta
    29281 tgtatcaggt ctacatcatt ttttaattca ttgaatttaa ttggttttgt aggccggata
    29341 aggcgttaac gccgcatccg gcataaacac aggcactttg tcaccaatct gaaaccagcg
    29401 tcataaaaag ccgattgcta ctcgacaaga cgtccgttac ccgagaattt tgctggcttc
    29461 gcgcgctaca ttatccatct cgtccaacag ttccagcagc tccggctcca aatcttcttc
    29521 tttagtaccg ctacgtagct gttgttcgat cagttggcag agattcttca tacgcggcac
    29581 accgctatag ccgcaactgc catgcagttt atgaatcaaa tcaaccaggc cttccgggtt
    29641 ttctccaacc agctgttcct caactttgtt gcgaacttca ggcaggaaat cgagtaacat
    29701 ttgcagcata tcgcgcgcta aatcggtttt tcctgctgcc tggcgtagtg ccagttgcca
    29761 gtcgagggtc gcattcgggt tcaccacaat ttcgttgact tcgggcgtca cgacgcgaga
    29821 ggaaataccg ctgccaggct tgtagcgcaa caacaaatta tgcaatcgct cttcttcaat
    29881 cggtttcgcc agataatcgc tcatccctgc gccaagcagc ttctcttttt gcccggccat
    29941 tgcatgcgcc gttaccgcga taaccggcgt ttgttgctga tgggggagct ggtggatgag
    30001 ctcgcaggcc cgaatgccat ccatgtcagg catttgaata tccattaaga tcaaatcgaa
    30061 cggcatctgt ttcgcccgtt caaccgcctg atgcccgcta tcgcaaagtt ccacatgttg
    30121 caccatatct tccagcaatg cgccgataag tttcaggtta gcggggttgt catcaactgc
    30181 catgactgtc attgccagct tgctttcatc ggttacaggc aaaagcgtgt tttgtttgtg
    30241 atgacaaaat tccgtcaggg caggcaacag gcgcgtaggt gttaatggtt tcagcagaca
    30301 cgcgccgata ccatcttgct tgagtttttc tgcattgact tgtgcatggc aaggaagtgc
    30361 cagcatcagg aaatcggtca tcgataccgc tttcgctaat cgctcatgtt gcattgttag
    30421 cggctcgcgg aaggtcaccg cgatgcctaa caacatcatg tcgtaatgcg cgggaggcag
    30481 cgcggagaac gttgggctat aaaccacttc cagcggcgtt tcacttaaaa tatccagcgt
    30541 gcattgcgct gctgcggagt ttggttcgac ataggccagg cgcttaccgg cgaggcactg
    30601 ggtggatggc ccttcgataa taatgttcgg gttcagatcg agattaatgt ggaaccagaa
    30661 agttgaaccg cgattcggct ggctatggaa cgaaatatcg ccgcccattt cattaaccag
    30721 tttttgtgta atcaccagcc ccagaccggt gccaccatga cggcgggaaa tactggcatc
    30781 agcctgtcgg aaggcctgga ataagcgcga ttgatcgcgt tcaggaatac caatgccggt
    30841 atcccgaatc tgcacttcaa tctgcacttt ggtattactc agcgcacgtt tttctaccag
    30901 aatatcaatg ttgccattct cggtgaattt aattgcattc cccaccaggt tagtgatgat
    30961 ttgctgtaat cgcagtgggt cgccgatcac gttatcaggc acgtcgcttt taatattgag
    31021 cgtcagttcc aaccctttat cgtgagaaga atgtgccagc agagtaacga cttcatccag
    31081 cgtgctgcgt agtgggaatg gaatactttc cagaatcagc ttacctgctt ccagtttcga
    31141 gaagtcgaga acatcattaa taattgccag caaattattt gccgaacgtt caatcgtatt
    31201 caggtgatcg cgctgcgttg gtgttaattc tgttttcagc gtcaggcggg taaagccaat
    31261 aacaccattc agtggtgtac gcagctcgtg tgacatattt gccagaaact cggatttaat
    31321 acgcgccgct tcctgggcgc gctttttcgc cagatctaac tcaacgttct gaatttccat
    31381 ctgctccagc gtttcacgca gatcggacgt cgcctggtcg atattgtgct gcatctcttc
    31441 gtgataagca gccagcgaca ttgccatcga gttgataccg tttttcagca tatccagctc
    31501 gccgagcata aatccttcca cccggctgtc gagttgcccg cgacggatgc ggtcgacggt
    31561 attcaccatg ttgcgaatcg gaccggttac atcgcgcatt aagcgccagc caaaaataag
    31621 cgcaataccg atacaaaaca gcatcatcac gctggaaata aagatctctt tatattgctg
    31681 caagcgaacc gatttaagat ccagctcaag cgcaatatat cccaacatat tttgactatt
    31741 tttggcatcg ctactgggcg attcgtcggg ggagtaactc tcagaaataa tcggcgtgcg
    31801 gaggatcata atatcgccgt cacgagtgac agtgagctgg cgaggaaacg gcacgttgct
    31861 gccgagctgc attgatgagg gatcaagatg aaaattggag gtgacaaaga gtcggttatt
    31921 ttcatcataa accgaaatcg cgcgaacaat atcggaatgg cgacgatgca gtacgcttat
    31981 taactgaccg atagattcgc gattttgcag gctcatgcca tattcagtag aaactgcaag
    32041 cggctcaata atgctggcac cggcatcttc cagttgacgc tgcaagtcgt tatagcgatg
    32101 cacgacgaaa aagatactca gcaataaacc aataaggacg gtcggtgcca ggatcagaat
    32161 catcatgcgt gcgcgcaggc tgtagttggt catggagttc cgttatggga caattaaggt
    32221 cacactgtta aattgagaaa gtctcgccaa tggcgcaatt ctactctgca aaacgacgca
    32281 cgacgacgcg tcagatcata accgtttcag tcaacgacct cgactctttt ggtcagggcg
    32341 tggcgcgaca taacggcaaa acgttattta tccccggatt attgccgcag gaaaacgcgg
    32401 aagttgctgt tactgaagat aaaaaacagt atgcccgcgc taaagtcgta cgccggttaa
    32461 gcgatagccc ggaacgcgaa acaccacgct gtcctcattt tggcgtatgc ggcggctgtc
    32521 agcaacaaca cgccagcgtg gatttacagc agcgaagcaa aagtgcggca ctcgcccgat
    32581 taatgaaaca cgatgtctct gaagtgatcg ccgatgttcc ctggggctat cgccgccgcg
    32641 cgcgtttaag tctgaactac ttaccgaaaa cacagcaact tcagatgggg tttcgcaaag
    32701 cgggctccag tgacattgtc gacgttaaac aatgccccat tttagtgccc caacttgaag
    32761 cattgctgcc caaagtcagg gcatgcctgg gcagcttaca agttatgcgc catcttggtc
    32821 atgttgaact ggtacaggca accagcggca cgctgatgat tttgcgccat accgcaccgc
    32881 taagttcggc agatcgcgaa aaactggaac gcttttcgca ttctgaaggt ctggatctgt
    32941 atctcgcccc cgatagtgag atactcgaaa ccgtctctgg tgagatgccc tggtatgact
    33001 caaacgggtt gcgcttaact tttagcccgc gcgattttat tcaggtcaat gcgggtgtga
    33061 accaaaaaat ggtagcgcgt gcgttggaat ggctggatgt gcaacctgaa gatcgcgtac
    33121 tggatctgtt ctgcggtatg ggcaacttta cactgccatt ggcgaaacaa gcagccagtg
    33181 tggtcggtgt agaaggtgtt ccggcgctgg tggaaaaagg ccagcagaat gcgcgtctta
    33241 atggcttaca gaatgtgacg ttttatcatg aaaatcttga agaagatgtc acaaagcagc
    33301 cgtgggcgaa aaacggcttc gataaagtgt tgctggaccc cgcgcgagca ggtgccgcag
    33361 gtgttatgca gcaaattata aaactggaac ctattcgtat agtttatgta tcctgtaatc
    33421 ctgcaacgct ggctcgggat agcgaagcgt tattaaaagc aggatatacc attgcgcgac
    33481 tggcgatgct ggatatgttc ccacacacgg gacatctgga atcgatggta cttttctcgc
    33541 gcgttaaata gttacgattt gccgatttcg gcaggtctgg tccctaaagg agaggacgat
    33601 ggttgcggta agaagtgcac atatcaataa ggctggtgaa tttgatccgg aaaaatggat
    33661 cgcaagtctg ggtattacca gccagaagtc gtgtgagtgc ttagccgaaa cctgggcgta
    33721 ttgtctgcaa cagacgcagg ggcatccgga tgccagtctg ttattgtggc gtggtgttga
    33781 gatggtggag atcctctcga cattaagtat ggacattgac acgctgcggg cggcgctgct
    33841 gttccctctg gctgatgcca acgtagttag cgaagatgtg ctgcgtgaga gcgtcggtaa
    33901 gtcggttgtt aaccttattc acggcgtgcg tgatatggcg gcgatccgcc agctgaaagc
    33961 gacgcacact gattctgttt cctccgaaca ggtcgataac gttcgccgga tgttattggc
    34021 gatggtcgat gattttcgct gcgtggtcat caaactggcg gagcgtattg ctcatctgcg
    34081 cgaagtaaaa gatgcgccgg aagatgaacg cgtactggcg gcaaaagagt gcaccaatat
    34141 ctacgcgccg ttggcgaacc gtcttgggat tgggcaactg aaatgggagc tggaagatta
    34201 ctgcttccgt tatctccacc cgaccgaata caaacgcatc gcaaaactgc tgcatgaacg
    34261 ccgtctcgac cgcgaacact atatcgaaga gtttgtcggc catctgcgcg ctgagatgaa
    34321 agctgaaggc gttaaagctg aagtgtatgg tcgtccgaaa cacatctaca gtatctggcg
    34381 caaaatgcag aaaaagaacc tcgccttcga tgagctgttt gatgtgcgtg cggtacgtat
    34441 tgtcgccgag cgtttacagg attgttatgc cgcactgggg atagtgcaca ctcactatcg
    34501 ccacctgccg gatgagtttg acgattatgt cgctaacccg aaaccaaacg gttatcagtc
    34561 tattcatacc gtggttctgg ggccaggtgg caaaaccgtt gagatccaaa tccgcaccaa
    34621 acagatgcat gaagatgcag agttgggtgt tgctgcgcac tggaaatata aagagggcgc
    34681 ggcagctggc ggcgcacgtt cgggacatga agaccggatt gcctggctgc gtaaactgat
    34741 tgcgtggcag gaagagatgg ctgattccgg cgaaatgctc gacgaagtac gcagccaggt
    34801 ctttgacgac cgggtgtatg tctttacgcc gaaaggtgat gtcgttgatt tgcctgcggg
    34861 atcaacgccg ctggacttcg cttaccacat ccacagtgat gtcggacacc gctgcatcgg
    34921 ggcaaaaatt ggcgggcgca ttgtgccgtt cacctaccag ctgcagatgg gcgaccagat
    34981 tgaaattatc acccagaaac agccgaaccc cagccgtgac tggttaaacc caaacctcgg
    35041 ttacgtcaca accagccgtg ggcgttcgaa aattcacgcc tggttccgta aacaggaccg
    35101 tgacaaaaac attctggctg ggcggcaaat ccttgacgac gagctggaac atctggggat
    35161 cagcctgaaa gaagcagaaa aacatctgct gccgcgttac aactttaatg atgtcgacga
    35221 gttgctggcg gcgattggtg gcggggatat ccgtctcaat cagatggtga acttcctgca
    35281 atcgcaattt aataagccga gtgccgaaga gcaggacgcc gccgcgctga agcaacttca
    35341 gcaaaaaagc tacacgccgc aaaaccgcag taaagataac ggtcgcgtgg tggtcgaagg
    35401 tgttggcaac ctgatgcacc acatcgcgcg ctgctgccag ccgattcctg gagatgagat
    35461 tgtcggcttc attacccagg ggcgcggtat ttcagtacac cgcgccgatt gcgaacaact
    35521 ggcggaactg cgctcccatg cgccagaacg cattgttgac gcggtatggg gtgagagcta
    35581 ctccgccgga tattcgctgg tggtccgcgt ggtggctaat gatcgtagtg ggttgttacg
    35641 tgatatcacg accattctcg ccaacgagaa ggtgaacgtg cttggcgttg ccagccgtag
    35701 cgacaccaaa cagcaactgg cgaccatcga catgaccatt gagatttaca acctgcaagt
    35761 gctggggcgc gtgctgggta aactcaacca ggtgccggat gttatcgacg cgcgtcggtt
    35821 gcacgggagt taggccgaaa tttgctcgta tctacaatgt agattgatat atactgtatc
    35881 tacatatgat agcggtttga ggaaagggtt atgatccaca gtagcgtaaa gcgttgggga
    35941 aattcaccgg cggtgcggat cccggctacg ttaatgcagg cgctcaatct gaatattgat
    36001 gatgaagtga agattgacct ggtggatggc aaattaatta ttgagccagt gcgtaaagag
    36061 cccgtattta cgcttgctga actggtcaac gacatcacgc cggaaaacct ccacgagaat
    36121 atcgactggg gagagccgaa agataaggaa gtctggtaat ggtaagccga tacgtacccg
    36181 atatgggcga tctgatttgg gttgattttg acccgacaaa aggtagcgag caagctggac
    36241 atcgtccagc tgttgtcctg agtcctttca tgtacaacaa caaaacaggt atgtgtctgt
    36301 gtgttcctta tacaacgcaa tcaaaaggat atccgttcga agttgtttta tccggtcagg
    36361 aacgtgatgg cgtagcgtta gctgatcagg taaaaagtat cgcctggcgg gcaagaggag
    36421 caacgaagaa aggaacagtt gccccagagg aattacaact cattaaagcc aaaattaacg
    36481 tactgattgg gtagtgttac taactcactt ctattctggt cacaggtttc cacctgacag
    36541 acccgcaatt ttcaggacaa ttcaatgaat caaatcgacc gtttgctcac tattatgcag
    36601 cgcctgcgcg atccggaaaa cggctgcccg tgggataaag agcagacatt tgccaccatt
    36661 gcgccttaca cccttgaaga aacctacgaa gtgctggacg ccatcgcccg tgaagatttt
    36721 gacgatcttc gcggtgaact gggcgatctg ctattccagg tggtgtttta cgcgcaaatg
    36781 gctcaggaag aagggcgctt tgactttaat gatatttgcg ctgctattag cgataaatta
    36841 gagcgtcgcc atccgcatgt ttttgctgat agttctgccg aaaacagtag tgaagtgctt
    36901 gcccgttggg agcaaatcaa aaccgaagag cgcgcgcaga aagcgcagca ttcggcgctg
    36961 gacgatattc ctcgtagttt accggcttta atgcgtgcgc aaaaaatcca gaaacgttgc
    37021 gccaacgttg gcttcgactg gacgacgctt ggtccggtag tcgataaagt ctacgaagag
    37081 atcgacgagg tgatgtacga agcgcggcag gctgttgtcg accaggctaa actggaggag
    37141 gaaatggggg acctgctgtt tgccacggtc aatctggctc gtcatttagg gacaaaagcg
    37201 gaaatcgcat tgcaaaaagc gaacgaaaaa tttgagcgtc gttttcgcga agtggagcgt
    37261 attgttgccg cgcgtggact ggaaatgaca ggtgttgacc tcgaaacaat ggaagaagtc
    37321 tggcaacagg taaaacggca ggaaattgat ctctaaggga attacgcggt caagcgccat
    37381 ttgtgtcatt ttttaaatga caagcgcttg atttgcgtca aaaacattta ccccaaaggg
    37441 gctattttct cactcctgat ttcaatagtg cgctggcgaa gaggagggat aatgaaagtt
    37501 tgtggcacag gtcatgttcg ggtatactgc tttcccgtcc tggttattcc atcgtctttt
    37561 caacctaact tctcaggttc agcatgacaa cgaactatat ttttgtgacc ggcggggtcg
    37621 tatcctctct gggtaaaggc attgccgcag cctccctcgc agccattctt gaagcccgtg
    37681 gcctcaatgt gaccatcatg aaactggatc cgtacatcaa cgtcgatcca ggtactatga
    37741 gcccaatcca acacggggaa gtgttcgtta ctgaagacgg cgctgaaacc gacctggacc
    37801 tggggcacta cgagcgtttc attcgtacca aaatgagccg ccgcaacaac ttcaccacgg
    37861 gtcgtatcta ctctgacgtt ctgcgtaaag aacgccgcgg tgactacctc ggcgcaaccg
    37921 tgcaggttat tccgcacatc actaacgcaa tcaaagagcg cgtgctggaa ggtggcgaag
    37981 gtcatgacgt agtactggta gaaatcggcg gtacagtagg tgatatcgaa tccttgccgt
    38041 tcctcgaagc gattcgccag atggctgttg aaattggccg tgagcacact ctgtttatgc
    38101 acctgacgct ggtgccgtac atggcagcgt ctggtgaagt caaaaccaaa ccgactcagc
    38161 actctgtaaa agagctgctc tccatcggta tccagcctga catcctgatt tgtcgttcag
    38221 atcgcgctgt tccggcgaac gaacgtgcga agattgcatt gttctgtaat gttccggaaa
    38281 aagcggttat ttctctgaaa gacgtcgatt ccatctataa aattccgggc ctgttgaaat
    38341 ctcaggggct ggacgattat atttgtaaac gattcagctt aaactgcccg gaagcgaatc
    38401 tgtccgaatg ggaacaggtt atcttcgaag aagcgaaccc ggtaagtgaa gtcaccatcg
    38461 gtatggtcgg caagtacatt gaactgccgg atgcttataa atcagtgatc gaagcactga
    38521 aacacggtgg gctgaagaat cgtgtcagcg tcaacatcaa actgatcgat tcacaagatg
    38581 ttgaaacgcg cggcgttgaa atccttaaag gtctggacgc aatcctcgta cctggcggtt
    38641 tcggctatcg tggcgtagaa ggcatgatta cgaccgcgcg ttttgcgcgt gagaacaata
    38701 ttccttatct gggcatttgc ctgggtatgc aggtggcgtt aattgattac gctcgccatg
    38761 ttgccaacat ggagaacgcc aactctacgg aatttgtgcc agactgtaag tacccggttg
    38821 tggcgctgat taccgagtgg cgcgatgaaa acggcaacgt tgaagttcgt agcgagaaga
    38881 gcgatctcgg cggtaccatg cgtctcggcg cacagcagtg ccagttggtt gacgatagcc
    38941 tggttcgcca gctgtacaat gcgccgacaa ttgttgagcg tcatcgtcac cgttacgaag
    39001 tcaacaacat gctgttgaaa cagattgaag atgcaggtct gcgcgttgcg ggccgttccg
    39061 gggatgatca gttggtcgag atcatcgaag ttccgaatca cccgtggttc gtggcttgcc
    39121 agttccatcc ggagtttact tctactccac gtgatggtca cccgctgttt gcaggctttg
    39181 tgaaagccgc cagcgagttc cagaaacgtc aggcgaagta agtaaaaaag ttagaacggc
    39241 aacgcgtacc ctgggtacgc gttgtttgtc tggagtttca gtttaactag tgacttgagg
    39301 aaaacctaat gtccaaaatc gtaaaaatca tcggtcgtga aatcatcgac tcccgtggta
    39361 acccgactgt tgaagccgaa gtacatctgg agggtggttt cgtcggtatg gcagctgctc
    39421 cgtcaggtgc ttctactggt tcccgtgaag ctctggaact gcgcgatggc gacaaatccc
    39481 gtttcctggg taaaggcgta accaaagctg ttgctgcggt aaacggcccg atcgctcagg
    39541 cgctgattgg caaagatgct aaagatcagg ctggcattga caagatcatg atcgacctgg
    39601 acggcaccga aaacaaatcc aaattcggcg cgaacgcaat cctggctgta tctctggcta
    39661 acgccaaagc tgctgcagct gctaaaggta tgccgctgta cgagcacatc gctgaactga
    39721 acggtactcc gggcaaatac tctatgccgg ttccgatgat gaacatcatc aacggtggtg
    39781 agcacgctga caacaacgtt gatatccagg aattcatgat tcagccggtt ggcgcgaaaa
    39841 ctgtgaaaga agccatccgc atgggttctg aagttttcca tcacctggca aaagttctga
    39901 aagcgaaagg catgaacact gcagttggtg acgaaggtgg ctatgcgccg aacctgggtt
    39961 ccaacgctga agctctggct gttatcgctg aagctgttaa agctgctggt tatgaactgg
    40021 gcaaagacat cactctggcg atggactgcg cagcttctga attctacaaa gacggtaaat
    40081 acgttctggc tggcgaaggc aacaaagcgt tcacctctga agaattcact cacttcctgg
    40141 aagaactgac caaacagtac ccgatcgttt ctatcgaaga cggtctggac gaatctgact
    40201 gggacggttt cgcataccag accaaagttc tgggcgacaa aatccagctg gttggtgacg
    40261 acctgttcgt aaccaacacc aagatcctga aagaaggtat cgaaaaaggt atcgctaact
    40321 ccatcctgat caaattcaac cagatcggtt ctctgaccga aactctggct gcaatcaaga
    40381 tggcgaaaga tgctggctac actgcagtta tctctcaccg ttctggcgaa actgaagacg
    40441 ctaccatcgc tgacctggct gttggtactg ctgcaggcca gatcaaaact ggttctatga
    40501 gccgttctga ccgtgttgct aaatacaacc agctgattcg tatcgaagaa gctctgggcg
    40561 aaaaagcacc gtacaacggt cgtaaagaga tcaaaggcca ggcataagac tgactttatc
    40621 tgatttaaaa atgccagcct ccgggctggc attttttatg gttgagttac cagcggccgg
    40681 atgcgccgcc acctccagaa gaaccgccac cgccgctaaa tccgccgcca cttgagcctc
    40741 caccggaacc gctgttgtct gaatgataac ttttacctga tgctcttttc tgaagtacac
    40801 ataaacaggt aaagaccata aatatggtga aagtaaaaaa gaaggaaaat acagcaatgg
    40861 aaaaaatttt atcggagaac aggaataaat ctaaaagata gattgctgaa atatagactc
    40921 cacttttaca tgcgcgacaa aaattactgc cttgatgaaa aaaccagaaa gggaagaaca
    40981 tcactgcaaa tacccaaaaa atagcaaagt aataatgatc actggaagac gctgattcac
    41041 tttctgaagg attagtcgga tattggtgtt gtgaagtgag ttggttattc aaagcgttta
    41101 tagctagctc taatccctga gcaaattttt gttgtttaaa tgcgggtatc atgttgctac
    41161 ggatgatatc tccagccaga gcatcggtta ccttttcttc cagcccataa cctacctgga
    41221 tgcggacagt acgatctgac caggcaacaa tgatcagtat cccatcatta cgtttggcat
    41281 ctcctaaacg ccaattgtca aaaactcttg tagcatattg ttcaattgtc tcgtctttgg
    41341 ttgtgggaac aattaataca gcaatttgca cttgggtatt attgaaaata gattctaact
    41401 tgtgcgtcag ttctttcttt tcttgtgagt ttaagctact cgtaagatcc gtaacttgtt
    41461 gctgtaattg tgggacaata gttggttggg ctgcaacaaa ggaacacacg aaagtgaaca
    41521 ttagtatgaa atagcgcatt ataaatttct cattaaatct ggataaataa attgacggcg
    41581 gcatcggtaa ttttagattc gttatcat
//
LOCUS       DAZGSP010000042        40439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZGSP010000042 DAZGSP010000000
VERSION     DAZGSP010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.042"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            114..557
                     /gene="cmtB"
                     /locus_tag="ACHK74_002324"
     CDS             114..557
                     /gene="cmtB"
                     /locus_tag="ACHK74_002324"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFP0221137.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            585..1973
                     /gene="cmtA"
                     /locus_tag="ACHK74_002325"
     CDS             585..1973
                     /gene="cmtA"
                     /locus_tag="ACHK74_002325"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFP0221138.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1988..3265
                     /gene="yggP"
                     /locus_tag="ACHK74_002326"
     CDS             1988..3265
                     /gene="yggP"
                     /locus_tag="ACHK74_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP0221139.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3262..4227
                     /gene="glpX"
                     /locus_tag="ACHK74_002327"
     CDS             3262..4227
                     /gene="glpX"
                     /locus_tag="ACHK74_002327"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFP0221140.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIDGADQTCNIIDSLH"
     gene            4249..4758
                     /gene="fumE"
                     /locus_tag="ACHK74_002328"
     CDS             4249..4758
                     /gene="fumE"
                     /locus_tag="ACHK74_002328"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFP0221141.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4755..5468
                     /locus_tag="ACHK74_002329"
     CDS             4755..5468
                     /locus_tag="ACHK74_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFP0221142.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5440..6117)
                     /locus_tag="ACHK74_002330"
     CDS             complement(5440..6117)
                     /locus_tag="ACHK74_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0221143.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKFPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(6111..6788)
                     /locus_tag="ACHK74_002331"
     CDS             complement(6111..6788)
                     /locus_tag="ACHK74_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0221144.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6776..7483)
                     /locus_tag="ACHK74_002332"
     CDS             complement(6776..7483)
                     /locus_tag="ACHK74_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFP0221145.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7484..8062)
                     /locus_tag="ACHK74_002333"
     CDS             complement(7484..8062)
                     /locus_tag="ACHK74_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP0221146.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTNPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8085..8516)
                     /locus_tag="ACHK74_002334"
     CDS             complement(8085..8516)
                     /locus_tag="ACHK74_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFP0221147.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8888..9907
                     /gene="epd"
                     /locus_tag="ACHK74_002335"
     CDS             8888..9907
                     /gene="epd"
                     /locus_tag="ACHK74_002335"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFP0221148.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9957..11120
                     /gene="pgk"
                     /locus_tag="ACHK74_002336"
     CDS             9957..11120
                     /gene="pgk"
                     /locus_tag="ACHK74_002336"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFP0221149.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11335..12414
                     /gene="fbaA"
                     /locus_tag="ACHK74_002337"
     CDS             11335..12414
                     /gene="fbaA"
                     /locus_tag="ACHK74_002337"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP0221150.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(12478..12576)
                     /locus_tag="ACHK74_002338"
                     /pseudo
     CDS             complement(12478..12576)
                     /locus_tag="ACHK74_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12772..13632
                     /gene="mscS"
                     /locus_tag="ACHK74_002339"
     CDS             12772..13632
                     /gene="mscS"
                     /locus_tag="ACHK74_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFP0221151.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13771..14406
                     /gene="argO"
                     /locus_tag="ACHK74_002340"
     CDS             13771..14406
                     /gene="argO"
                     /locus_tag="ACHK74_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFP0221152.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14499..15239
                     /gene="yggE"
                     /locus_tag="ACHK74_002341"
     CDS             14499..15239
                     /gene="yggE"
                     /locus_tag="ACHK74_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFP0221153.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15406..16302
                     /gene="ygfI"
                     /locus_tag="ACHK74_002342"
     CDS             15406..16302
                     /gene="ygfI"
                     /locus_tag="ACHK74_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0221154.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(16299..17777)
                     /gene="scpC"
                     /locus_tag="ACHK74_002343"
     CDS             complement(16299..17777)
                     /gene="scpC"
                     /locus_tag="ACHK74_002343"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFP0221155.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGEIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17801..18586)
                     /gene="scpB"
                     /locus_tag="ACHK74_002344"
     CDS             complement(17801..18586)
                     /gene="scpB"
                     /locus_tag="ACHK74_002344"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFP0221156.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18597..19592)
                     /gene="meaB"
                     /locus_tag="ACHK74_002345"
     CDS             complement(18597..19592)
                     /gene="meaB"
                     /locus_tag="ACHK74_002345"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFP0221157.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19585..21729)
                     /gene="scpA"
                     /locus_tag="ACHK74_002346"
     CDS             complement(19585..21729)
                     /gene="scpA"
                     /locus_tag="ACHK74_002346"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFP0221158.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(21933..22826)
                     /gene="argP"
                     /locus_tag="ACHK74_002347"
     CDS             complement(21933..22826)
                     /gene="argP"
                     /locus_tag="ACHK74_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFP0221159.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            22968..23153
                     /locus_tag="ACHK74_002348"
     CDS             22968..23153
                     /locus_tag="ACHK74_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221160.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            23209..23868
                     /gene="rpiA"
                     /locus_tag="ACHK74_002349"
     CDS             23209..23868
                     /gene="rpiA"
                     /locus_tag="ACHK74_002349"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFP0221161.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24124..25356
                     /gene="serA"
                     /locus_tag="ACHK74_002350"
     CDS             24124..25356
                     /gene="serA"
                     /locus_tag="ACHK74_002350"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP0221162.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25585..25644
                     /gene="ibsC"
                     /locus_tag="ACHK74_002351"
     CDS             25585..25644
                     /gene="ibsC"
                     /locus_tag="ACHK74_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFP0221163.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            25977..26036
                     /locus_tag="ACHK74_002352"
     CDS             25977..26036
                     /locus_tag="ACHK74_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP0221164.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26137..26739)
                     /gene="fau"
                     /locus_tag="ACHK74_002353"
     CDS             complement(26137..26739)
                     /gene="fau"
                     /locus_tag="ACHK74_002353"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFP0221165.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26760..26943)
                     /gene="ssrS"
                     /locus_tag="ACHK74_002354"
     ncRNA           complement(26760..26943)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHK74_002354"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(26985..27314)
                     /gene="zapA"
                     /locus_tag="ACHK74_002355"
     CDS             complement(26985..27314)
                     /gene="zapA"
                     /locus_tag="ACHK74_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP0221166.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27482..28060
                     /gene="ygfB"
                     /locus_tag="ACHK74_002356"
     CDS             27482..28060
                     /gene="ygfB"
                     /locus_tag="ACHK74_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFP0221167.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            28086..29411
                     /gene="pepP"
                     /locus_tag="ACHK74_002357"
     CDS             28086..29411
                     /gene="pepP"
                     /locus_tag="ACHK74_002357"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFP0221168.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYTYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPIVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29408..30586
                     /gene="ubiH"
                     /locus_tag="ACHK74_002358"
                     /gene_synonym="visB"
     CDS             29408..30586
                     /gene="ubiH"
                     /locus_tag="ACHK74_002358"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFP0221169.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30609..31811
                     /gene="ubiI"
                     /locus_tag="ACHK74_002359"
                     /gene_synonym="visC"
     CDS             30609..31811
                     /gene="ubiI"
                     /locus_tag="ACHK74_002359"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFP0221170.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHVVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31885..31968)
                     /locus_tag="ACHK74_002360"
     CDS             complement(31885..31968)
                     /locus_tag="ACHK74_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221171.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            32258..33352
                     /gene="gcvT"
                     /locus_tag="ACHK74_002361"
     CDS             32258..33352
                     /gene="gcvT"
                     /locus_tag="ACHK74_002361"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFP0221172.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            33376..33765
                     /gene="gcvH"
                     /locus_tag="ACHK74_002362"
     CDS             33376..33765
                     /gene="gcvH"
                     /locus_tag="ACHK74_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFP0221173.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            33884..36757
                     /gene="gcvP"
                     /locus_tag="ACHK74_002363"
     CDS             33884..36757
                     /gene="gcvP"
                     /locus_tag="ACHK74_002363"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFP0221174.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFNVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            37018..37761
                     /gene="ygfF"
                     /locus_tag="ACHK74_002364"
     CDS             37018..37761
                     /gene="ygfF"
                     /locus_tag="ACHK74_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0221175.1"
                     /translation="MVIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(37818..39251)
                     /gene="bglA"
                     /locus_tag="ACHK74_002365"
     CDS             complement(37818..39251)
                     /gene="bglA"
                     /locus_tag="ACHK74_002365"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFP0221176.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGSWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     AVFERYKHKVKYWMTFNEINNQRNWRTPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            39296..39607
                     /gene="yqfB"
                     /locus_tag="ACHK74_002366"
     CDS             39296..39607
                     /gene="yqfB"
                     /locus_tag="ACHK74_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFP0221177.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVRLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            39771..40430
                     /gene="yqfA"
                     /locus_tag="ACHK74_002367"
     CDS             39771..40430
                     /gene="yqfA"
                     /locus_tag="ACHK74_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFP0221178.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
BASE COUNT         9637 a        10074 c        10904 g         9824 t
ORIGIN      
        1 taattcactg ttttcaaaac atcggtttcc ctgctcaatt gctttcatta aaccgcacga
       61 gtattttatt tttaccgccg gacggtaccc taaaaataaa ataaggataa tttatgcggc
      121 ttagtgatta ttttccagaa tcatcaatct cagttataca ttcagcaaaa gattggcagg
      181 aagctatcga tttctcgatg gtatcattgc tggataaaaa ctatatcagc gagaattaca
      241 ttcaggcaat taaagattcc accattaaca atggccctta ttatattctc gcaccaggcg
      301 tggcaatgcc tcatgcgcga ccggaatgtg gggcgcttaa aaccgggatg tctttgacat
      361 tacttgaaca aggtgtttat tttccgggga atgacgaacc aattaaatta ctcatcggac
      421 tctctgctgc cgatgccgat tcgcacattg gcgctattca ggcgttaagt gaattactgt
      481 gcgaagaaga aatactcgaa caactcttaa cagcatcatc agaaaaacaa ttagcggaca
      541 ttatcagccg cggataatta ctctctcctc tacaggataa tattatggaa aacaagtctg
      601 ctcgtgcaaa ggtccaggct tttgggggct ttttgactgc aatggtcatc cccaatattg
      661 gtgcttttat tgcctggggc ttcataactg cgttatttat tcccaccggt tggctgccga
      721 atgaacattt cgccaaaatt gtcggcccga tgattaccta tttattgccc gtgatgattg
      781 gttctactgg tggtcatctg gtcggcggta aacgcggggc ggtcatgggc ggaataggta
      841 ctattggtgt gatcgttggc gcagagatcc cgatgttcct tggctcaatg atcatggggc
      901 cgctcggtgg gttggtcata aaatatgtcg ataaggcact ggaaaaacgc atacctgccg
      961 gttttgagat ggttatcaat aacttctcat taggtatcgc ggggatgctc ctttgtctgc
     1021 tgggttttga agtgatcggc ccggcggtgt taattgccaa tactttcgtc aaagagtgta
     1081 tcgaggcgct ggtacatgcg ggttatctgc ctctgctgtc agtcatcaat gaaccggcga
     1141 aagtgctttt ccttaataat gcgatcgatc agggcgtcta ttatccgctg ggaatgcaac
     1201 aggcttcggt taacggtaaa tccatcttct ttatggtggc ctctaaccca ggtccgggcc
     1261 tggggctgct gctggcgttt accttgtttg gtaaagggat gagtaaacgt tctgcgcccg
     1321 gggcgatgat tattcacttc ctcggtggga tccacgaact gtatttccca tatgtgctga
     1381 tgaagccgct gactattatt gccatgattg cgggcggtat gtctggtacc tggatgttta
     1441 acttactgga cggtggtctg gtggctggcc caagtccggg gtctatcttt gcttacctgg
     1501 cactgacgcc gaaaggctcg ttcctggcga caattgccgg tgttacggta ggtaccctgg
     1561 tgtcctttgc tattacttcg ctgatactga agatggaaaa aacggtggaa acggagagcg
     1621 aagatgagtt tgctcagtca gccaatgcgg ttaaggcgat gaaacaagag ggtgcattct
     1681 cgttaagcag ggttaagcgt atcgcctttg tttgcgatgc ggggatgggc tccagtgcga
     1741 tgggcgcgac caccttccgt aaacgcctgg aaaaagcggg gctggcaata gaagtaaaac
     1801 attacgcgat agaaaacgtg cctgcggatg ccgatatcgt cgtcactcac gccagtctgg
     1861 aagggcgcgt gaaacgtgtg acggataaac cgctgatatt gattaataac tatattggcg
     1921 atccaaaact cgacacttta tttaatcaat taaccgccga acataaacac tgattacaga
     1981 ggtaaaaatg aaaaccaaag ttgctgctat ttatggcaag cgggatgtcc gtctgcgcga
     2041 atttgaactg ccagaaatta ccgataatga actgttagtg agtgtaattt ctgacagcgt
     2101 ctgtttatcg acctggaaag cggcgttact cggtagtgaa cataaacgcg tacccgacga
     2161 tttagaaaat catccggtca ttaccgggca tgaatgtgcc ggggttattg tcgaagtggg
     2221 taaaaatctc actggcaaat ataaaaaagg ccagcgtttt gtattgcaac cggcgatggg
     2281 gttaccaagc ggatattcag caggctacag ctacgaatat tttggcggta acgccaccta
     2341 tatgattatt cccgaaatcg ccattaattt gggctgcgta ttaccgtatc acggctctta
     2401 ttttgctgcg gcgtcgctgg cagagcctat gtgctgcatt attggtgctt atcatgccaa
     2461 ttatcacacc acgcaatatg tttatgagca tcgcatgggc gtcaaacctg gcggcaatat
     2521 tgcactgctg gcgtgtgcgg gtccgatggg cattggcgct atcgattacg ccattaacgg
     2581 tggcatacaa ccgtcacggg tggtggtggt cgatatcgac gacaaacgcc tggcgcaggt
     2641 gcagaagctg ctgccggtgg atctggcggc cagtaaaggc attgagctgg tgtatgtgaa
     2701 taccaaaggg atgagcgatc cggtccagac gctgcgggcg ctgacaggag atgtcgggtt
     2761 cgatgacatt tttgtttatg cggcggtgcc tgctgtcgtt gagatggctg acgaattact
     2821 ggcggaagat ggctgtctga acttctttgc cgggccgacg gataaaaact tcaaagtgcc
     2881 gtttaatttc tacaacgtcc attacaacag cacgcacgta gtcggtacat ccggcggttc
     2941 aacggacgac atgaaagagg cgattgcgtt aagcgccacg gggcagttgc agccgtcctt
     3001 tatggtcacg catatcggtg ggctggatgc ggtgccagat accgtgctca atctgccgga
     3061 tatccctggc ggtaaaaaac tcatttataa cggcgtgacc atgccgctca ctgccattgc
     3121 cgattttgcc gaaaaaggca aaaccgatcc gctgtttaaa gagttggcgc ggctggttga
     3181 ggaaacgcac ggcatctgga atgaacaggc cgagaaatat ctgctggcac aatttggcgt
     3241 tgatatcggg gaggccgcgc aatgatgtcc ctggcgtggc cattatttcg cgttacggaa
     3301 caggcagcgt tggctgcctg gccgcaaacc ggatgtggcg acaaaaataa aattgatggc
     3361 ctggcggtca ccgcgatgcg ccaggcatta aacgacgtcg cttttcgtgg acgagtggtt
     3421 atcggcgaag gagaaattga ccatgcgccg atgctgtgga ttggcgaaga ggtgggcaaa
     3481 ggcgatgggc cagaagtcga tatcgcggtt gacccgattg aaggcacgcg gatggtagcg
     3541 atggggcaga gcaatgcgct ggcggtcatg gctttcgccc ctcgagatag cctgttacat
     3601 gcgcccgata tgtatatgaa aaagctggtt gttaatcgac tggctgccgg ggcgattgat
     3661 ctgtctttgc ctctggcgga caacttgcgt aacgtggcgc gggcgttagg taaaccgctg
     3721 gacaagctgc gcatggttac gctggataaa ccgcgcctga gtgccgcgat tgaagaagca
     3781 acacagctgg gcgtgaaggt tttcgctctg ccggatggcg atgtcgccgc cagcgtgctg
     3841 acctgctggc aggataatcc ttacgacgtg atgtacacca tcggcggtgc gccagaaggc
     3901 gtgatttctg cctgtgccgt taaagcatta ggtggggata tgcaggccga gctgattgac
     3961 ttttgtcagg ctaaagggga ttacacggaa aatcggcaga ttgcagagca ggagcgcaag
     4021 cgttgtaagg caatgggcgt tgacgttaac cgtgtgtact cgctcgatga actggtgagg
     4081 gggaacgata tcctctttag cgctacgggc gtgacgggcg gcgagttggt gaacggtatc
     4141 caacagacgg cgaatggggt gcggacgcag acattactga tcgacggcgc ggaccaaacg
     4201 tgtaatataa tagactccct gcattgatgg tgatctatcg gaagaccaat ggcgacgctg
     4261 acagaagatg atgtgcttga gcaactggat gcacaggaca atttattttc atttatgaaa
     4321 actgcgcatt ctattttgct ccaggggata cgccagtttc tgccgtcgct gtttgtcgat
     4381 aacgatgaag agatcgtcga atatgcagtg aagccgttac tcgcccaaag cggcccgctt
     4441 gacgatattg atgttgcgct gcgtttgatt tatgcgctgg ggaaaatgga taaatggctg
     4501 tatgccgata tcacgcactt ttcccagtac tggcattacc tgaacgaaca ggatgaaacg
     4561 cccggatttg ccgatgacat gacctgggat tttatcagca acgtcaatag cataacctgc
     4621 aatgcgacgc tctatgacgc gttaaaagcg atgaagttcg ccgatttcgc cgtctggtca
     4681 gaggcgcgtt ttagcggaat ggtcaaaacg gcgctgacgc tggcagtaac gaccacctta
     4741 aaggaattaa cgccgtgaaa attgaattaa cggtgaatgg gctgaacgtg caagcgcagt
     4801 accatgatga agagatagaa cgcgtgcata aaccgttgct gcgtatgctg gcggccttac
     4861 agacggtaag tccgcagcga cgtacggtgg tttttctttg cgctccgccg gggacgggta
     4921 aatctacctt aactaccttc tgggaatatc tcgcgcaaca agaccctgaa ttacccgcta
     4981 tccagacgct cccgatggat ggttttcacc attacaatag ctggctggat gcgcatcaat
     5041 tgcgcccctt caaaggcgca ccagagacat ttgacgttgc gaaactggcg gaaaatctgt
     5101 gccaggtagt ggaaggggat tgtacgtggc cgcagtatga tcgacaaaag catgatcctg
     5161 ttgaagatgc gttgcacgtt accgcaccac tcgtcatcgt cgaaggaaac tggttgttgc
     5221 tggacgacga gaagtggtgc cagcttgcgc aattttgtga tttctcgatc tttatcaacg
     5281 cgccagctac tgcgttgcgg gaacgactgg tagggcgcaa gctggcggga ggggtatcgc
     5341 tggcagacgc agaagctttt tatgaccgta ccgacgggcc gaatgttcgc cgggggctgg
     5401 aagagagtct gcctgcaaat ttgaccttaa tgatgaccgc tacgggtgaa tatcgtctgg
     5461 tggattaacg ttcctgatta gaccatcttc cagtcgcacc acacgagaga aatgacgccg
     5521 ggtaaatgcg gcgtcatgac tgattgctac gacgctcgtg cctcgctggc cgcacttctc
     5581 cagccagctt tcaaacacgc tcaaccaatt ttcgtcaaaa tcccgactcg gctcgtccag
     5641 cagcaatagt ggcggagaga gtgcttccag gcaggcgacg gcgaccatcc gtcgctgcgc
     5701 actatgcaga tccaacgggt gcgagttggc agtgtctgct aactgacaac attgcagggc
     5761 agcatgggta cgttgagtga tttcatccgc cgggaatttt tgcagtttca aaccaaatgc
     5821 gatctcgtca gcgacggtgc tgtgaaaaag ttggttttcc gcttcctgaa acagtacgcc
     5881 aacctttgcc gcgcgctggc ggtttttcag gtttttcatc gcctgttgtt gcagcataac
     5941 cgtaccggct gtgggcgtaa gaagacctgc cattactcgt aacagcgtcg attttcccgc
     6001 gccattgtcg ccggtcagcg ccaaccattc accttgtttg agttgcagag aaatgtcgca
     6061 aagacaatcc gttgccgcgc caggccagcg ataagatatc ttatttaacg ttaacatagt
     6121 ggctggagcg ctccgttttg caattgccac gcttgctggc agtagttcag gaaaggtgtg
     6181 cgatgacgtt caaaaagaat aatcagcgaa ccacgttcaa acgcccagtg ttgtaagcgc
     6241 tgcaacagca tttcgctggc ctgcggagtc aggcggctaa aggcttcatc aagaattaag
     6301 agctttggct gcatcgcgat ggcgcaggcg atgaccacgc gctgtgtctc tccaccggag
     6361 agtgttgccg ggtgacgatg gcgcaacggc tggcactcgg tcagagtcag ggcggcatca
     6421 atccgcgcca taatctctgc ttcagcaaga cacagatttt ctggcccaaa ggcaacttct
     6481 tcttccacgc taaaggtaca gccggagagc tgcaaatagg gcgattgctg gacgagctgg
     6541 atggtggcgg attgttcatt aagcgggaga tgcccaatgg gcgtaccgag gagcgtcccc
     6601 gtgcctgtta tctctcctgg cagaaagtcc ggataccagc ccgccatcag ctgtgccagc
     6661 gtacttttgc cactgccgtt gtcaccaaag atggcgacca tccccggttt gacatagtga
     6721 aagtcataac agaacggagg atgcgttgag tgtgtcgggc aataacggaa ctgttctaac
     6781 gtaaccatat ccacgctccg aattctgtca gcatcagcaa gatcatggtg taacgcgcta
     6841 cgcgttgtag ggtgctatcg gctggtgccc acaacgttgt tcgtcggttg tggatccgaa
     6901 atgcccgcat atccagcgcc gcaccacgga cagccagatc gttaagcgca ttgtgtgtta
     6961 gcggaataat taacgcgggc atcgcacgta aacgctgata ccagccctca tccagcggca
     7021 cgccgcgggc acgctgcgct tcatgaataa tcgctaattg ccgttttaac tgctcaacga
     7081 ccaataacgg cccggcaaac agataagcga cgcctggcgg caggcgagaa gcaaacagag
     7141 cgcgaataaa acattgcacg gggacgtact gcatccacaa ctgtgaggtt gaaacaatcg
     7201 ccagaatccg caaccagagc gtaatagcat gcgaccaccg ttctggcgag cgtggcgtgc
     7261 cgcttagcca ttctggtagc cagccaccgt gtaccagcca cagcccggct cccagcgaga
     7321 acatcagcca gacgacatat tttgcccgcc gccgcgtggc ctttagcgca ataaggcaga
     7381 aaaaggtcgc cgcgctatag atcggcaaaa tggtctgcgc gggcaggata agcgtggtgc
     7441 aggccgccaa tgcccacagt gttaatgagg taaatggatg catttagcgc acggcagcca
     7501 ttgccggaaa atggcgcgtg gtgcgaatcg gcagctggcg cagtaaaagc cagacaatca
     7561 ccgctgtaag aattttatcg accaggttcg cgcctatcac cgtaatcgcc accgactcca
     7621 ccaggttttg ccccatcgag tgcatccagg caacgaacaa atcggctccg ctaccggtga
     7681 caccgccgaa cagcgcagta cgcagcggta cagcgacgac cgttaccgca agcgtaataa
     7741 tcacgccgct gacaacgact tttggcaggg tgcgaaacca acccgctcgc gccaggcaac
     7801 cggcgaccag gccgatgacc atcgcgacgg gtgcaaatgc ggcggcgatc gggttggtca
     7861 gcaatcccca gagtaaattg gtcagtaagc cggtcagcat tccgatcacc ggaccaagca
     7921 gtaccgcgct aataagtgtg ccgatggagt cgagaaatat aggtaatttg accatgctgg
     7981 ccagttgccc gccgatcata ttgatggcga tagatataac gatcaacacc agtgcctgac
     8041 tggaaaagtg gctacgcgcc ataagaaaac ctctttgaaa gtagttattt aacgggggaa
     8101 atatgcaact cgtcgtaacc agaaagcgcg caaggttggc ggctaaacgc cagttcctca
     8161 aggctaccga tcaccagttc aagcgtggtc cgcacggtac agccgggcat catatgaccg
     8221 ccaagcatcg tgccgtgtgg atcggaaacg cagagatgaa gatgctcacc actctgttcc
     8281 agcgtgccgt ttagtgagat cacttcgaat gtaccgttca ggagcgtggt gccttcttgc
     8341 ccggcatagc gcaaggcgac gtcagtcagg ctaccggtac agcctgcaat ccaggcggca
     8401 tgaagttgat gctgctgtgc aaaagcgtgt agctgggaaa aaacttcctg cccggggagg
     8461 aggcgcagtg cgtaaaagcg tgcggaggaa gaatatgggt agggcaatgt catcattgga
     8521 gtactccgaa actcggacgt attgcggata atatcgacgc tgcatcagtc atattctgat
     8581 ataagtcagg gtttgtgacg gcacaacgaa aagattttgc tacgcttatt cgtgattctg
     8641 caaagtggaa taaattccac aggggcgggg ctgaaaacgt gacgtaagtc agataattgc
     8701 tttctttgac tggacaatca ttccttttat tccacgtttc gcttatccta gctgaagcgt
     8761 ttcagtcgat taaatgttcg acaattaacc aatcagtcgc agtttgcgac aggtaaggtt
     8821 tccccggacg atttgctgga ttactctgtc tggcaaattt gctcagggaa aaccttgcag
     8881 gagatctatg accgtacgcg tagcgataaa tggcttcggt cgcatcgggc gtaatgtggt
     8941 tcgtgctttg tatgaatccg gacgccgggc ggaaattacc gtggtggcaa tcaacgaact
     9001 ggcggatgct gcgggcatgg cgcatttgtt gaaatatgac accagccatg gccgttttgc
     9061 atgggaagta cgacaggaac gcgatcaact ttttgttggt gatgacgcca tccgcgtatt
     9121 gcatgaacgt tcactgcaat cgctcccctg gcgtgaactt ggcgttgatg tagtcctcga
     9181 ctgcaccggc gtatatggct cccgcgagca tggcgaagcg catattgccg ccggggccaa
     9241 aaaagtgctc ttttcacatc ctggcagtaa cgatctcgac gcgaccgttg tttacggcgt
     9301 caatcaggat caacttcgtg cggaacaccg catcgtttct aacgcttcct gtaccacgaa
     9361 ttgcataatt cccgtcatca aattgttaga tgatgcgtac ggtattgagt ccggcactgt
     9421 gaccacaatt cactccgcca tgcacgatca acaggttatt gatgcatacc atcctgacct
     9481 gcgtcgcacc cgggcagcca gccagtcgat cattccggtc gatactaaac tggccgccgg
     9541 tatcacacga ttttttccgc aatttaacga tcgctttgaa gcgattgcgg tacgtgtgcc
     9601 aaccataaat gtgacggcaa tcgatttaag cgtgacggtg aagaaacctg taaaagccaa
     9661 tgaagtcaac ctgttgctgc aaaaagcagc acaaggtgca tttcatggta tagttgacta
     9721 tacggaattg ccgttggtct ctgtagattt taaccacgat ccgcacagtg ccattgtcga
     9781 tggcacccaa acccgggtca gtggcgcaca cctgatcaaa acgttggtct ggtgcgataa
     9841 cgaatggggc tttgctaacc gaatgctcga cacgacgtta gctatggcta ctgttgcttt
     9901 caggtaagac gcaagcagcg tctgcaaaac ttttagaatc aacgagagga ttcaccatgt
     9961 ctgtaattaa gatgaccgat ctggatcttg ctgggaaacg tgtatttatc cgtgcggatc
    10021 tgaacgtacc agtaaaagac gggaaagtaa ccagcgacgc gcgtatccgt gcttctctgc
    10081 cgaccatcga actggccctg aaacaaggcg caaaagtgat ggtaacttcc cacctgggtc
    10141 gtcctaccga aggcgagtac aacgaagaat tctctctgct gccggttgtt aactacctga
    10201 aagacaaact gtctaacccg gttcgcctgg ttaaagatta cctcgacggc gttgacgttg
    10261 ctgaaggtga actggttgtt ctggaaaacg ttcgcttcaa caaaggcgag aagaaagacg
    10321 acgaaaccct gtccaaaaaa tacgctgcac tgtgtgacgt gttcgtaatg gacgcattcg
    10381 gtactgctca ccgcgcgcag gcttctactc acggtatcgg taaattcgct gacgttgcgt
    10441 gcgcaggccc gctgctggca gctgaactgg acgcgctggg taaagcactg aaagaacctg
    10501 ctcgcccgat ggtggctatc gttggtggtt ctaaagtatc taccaaactg accgttctgg
    10561 actccctgtc taaaatcgct gaccagctga ttgttggtgg tggtatcgct aacaccttta
    10621 tcgcggcaca aggccacgat gtgggtaaat ccctgtacga agctgacctg gttgacgaag
    10681 ctaaacgtct gctgaccacc tgcaacatcc cggttccgtc tgatgttcgc gtagcaaccg
    10741 agttctctga aactgcaccg gctaccctga aatctgttaa cgatgtgaaa gctgacgagc
    10801 agatcctgga tatcggtgat gcttccgctc aggaactggc tgaaatcctg aagaatgcga
    10861 aaaccattct gtggaacggt ccggttggcg tgttcgaatt cccgaacttc cgcaaaggta
    10921 ctgaaatcgt ggctaacgct atcgcagaca gcgaagcgtt ctccatcgct ggcggcggcg
    10981 acactctggc agcaatcgac ctgttcggca ttgctgacaa aatctcctac atctccactg
    11041 gcggcggcgc attcctcgaa ttcgtggaag gtaaagtact gcctgcagta gcgatgctcg
    11101 aagagcgcgc taagaagtaa aaaatcacag ggcagggaaa cctgcccttg tttcagcgcg
    11161 cttttagagc acgcattttt tcatactcta aataattcga gttgcaggaa ggcgacaaga
    11221 gagtgaatcc ccaggagctt acataagtaa gtgactgggg tgagcgaacg cagacgcagc
    11281 acatgcaact tgaagtatga tgagtataag gcccgacgat acaggacaag agacatgtct
    11341 aagatttttg atttcgtaaa acctggcgta atcactggtg atgacgtaca gaaagttttc
    11401 caggtagcaa aagaaaacaa cttcgcactg ccagcagtaa actgcgtcgg tactgactcc
    11461 atcaacgccg tactggaaac tgctgctaaa gttaaagcgc cggttatcgt tcagttctcc
    11521 aacggtggtg cttcctttat cgctggtaaa ggcgtgaaat ctgacgttcc gcagggtgct
    11581 gctatcctgg gcgcgatctc tggtgcgcat cacgttcacc agatggctga acattatggt
    11641 gttccggtta tcctgcacac tgaccactgc gcgaagaaac tgctgccgtg gatcgacggt
    11701 ctgctggacg cgggtgaaaa acacttcgca gctaccggta agccgctgtt ctcttctcac
    11761 atgatcgacc tgtctgaaga atctctgcaa gagaacatcg aaatctgctc taaatacctg
    11821 gagcgtatgt ccaaaatcgg catgactctg gaaatcgaac tgggttgcac cggtggtgaa
    11881 gaagacggcg tggacaacag ccacatggac gcttctgcac tgtacaccca gccggaagac
    11941 gttgattacg catacaccga actgagcaaa atcagcccgc gtttcaccat cgcagcgtcc
    12001 ttcggtaacg tacacggtgt ttacaagccg ggtaacgtgg ttctgactcc gaccatcctg
    12061 cgtgattctc aggaatatgt ttccaagaaa cacaacctgc cgcacaacag cctgaacttc
    12121 gtattccacg gtggttccgg ttctactgct caggaaatca aagactccgt aagctacggc
    12181 gtagtaaaaa tgaacatcga taccgatacc caatgggcaa cctgggaagg cgttctgaac
    12241 tactacaaag cgaacgaagc ttatctgcag ggtcagctgg gtaacccgaa aggcgaagat
    12301 cagccgaaca agaaatacta cgatccgcgc gtatggctgc gtgccggtca gacttcgatg
    12361 atcgctcgtc tggaaaaagc attccaggaa ctgaacgcga tcgacgttct gtaagatatt
    12421 cctttctgct tatctcaagg cccgctctgc gggtcttttt ttcgccaaaa gcaaaattgc
    12481 ctgatgcgct acgcttatca ggcctacacg tatcctgcaa tatattgaat ttgcaggttt
    12541 ttgtaggccg gataaggcgt tcacgccgca tccggcatga caaagcgcac attgatatct
    12601 gtctgagccc cgctctgcgg ggattttttc gccaaaagca aggtgattca gatgagaaat
    12661 ctgtgatcta tttggcaaaa ttatgcttta ttgtttaccc ttgtcagact gcccgtcata
    12721 aggcggcgga gtgtatttct ccattttgag tcagttgaaa aggaatattg aatggaagat
    12781 ttgaatgttg tcgatagcat aaacggcgcg ggaagctggc tggtagctaa ccaggcgctg
    12841 ctgctaagtt atgcagtaaa catcgtggcg gcactcgcga tcatcatcgt tggtttgatt
    12901 atcgcgcgga tgatttccaa cgcggtgaat cgcctgatga tctcccgtaa aatcgatgcc
    12961 actgttgctg attttctttc tgcattagtc cgttacggta ttatcgcctt tacgctaatc
    13021 gctgccctgg gacgcgtggg tgtacaaaca gcgtcagtca ttgctgtact cggtgccgca
    13081 ggcttagctg ttggtctggc tttgcagggg tcactttcta acctggccgc tggcgtgtta
    13141 cttgtcatgt tccgcccgtt ccgtgccgga gaatatgttg acctcggcgg cgtagccggt
    13201 actgtgctga gtgtgcagat tttctccacc accatgcgta ctgcagacgg taaaattatc
    13261 gttattccga acggtaaaat tattgccgga aatattatta acttctcccg cgagccagtt
    13321 cgccgtaacg aatttattat tggcgtggcg tatgattccg atatcgatca ggttaagcag
    13381 atcctgacca atattatcca gtctgaagat cgcattttga aagatcgcga aatgactgtg
    13441 cgcctgaacg aacttggtgc atcgtcgatt aatttcgtgg tccgcgtctg gagcaacagc
    13501 ggcgatctgc aaaacgtgta ctgggatgtg ctggagcgta ttaaacgtga atttgatgcc
    13561 gccggtatca gcttcccgta cccgcaaatg gatgtgaact ttaagcgggt gaaagaagac
    13621 aaagctgcgt aatcaacgct gaggccagat aatactctct ggcctctctc ttattagtta
    13681 ttctgattgc taattaatat tatcaatttc cgctaataac aatcccgcga tatagtctct
    13741 gcatcagata cttaattcgg aatatccaac gtgttttctt attactttca aggtcttgct
    13801 cttggggcag ctatgattct gccgctcggc ccacaaaatg cttttgtgat gaatcagggc
    13861 attcgtcgtc agtaccacat tatgattgcc ttactttgcg ctatcagcga tttggtcctg
    13921 atttgcgcgg ggatttttgg tggtagcgcg ttattgatgc agtcgccgtg gttgctggcg
    13981 ctggtcacct ggggcggcgt agtcttcttg ctgtggtatg gttttggcgc ttttaaaaca
    14041 gcgatgagta gtaatattga gttagctagt gccgaagtcc tgaagcaagg cagatggaaa
    14101 attatcgcca ccatgttggc agtgacctgg ctgaatccgc atgtttacct ggatactttt
    14161 gttgtactgg gcagccttgg cgggcagctt gacgtcgaac caaaacgttg gtttgcgctc
    14221 gggacaatta gcgcctcttt cctgtggttc tttgggctgg ctattctcgc agcctggctg
    14281 gcaccgcgcc tgcgcacggc aaaatcacag cgcattatca atctggtagt gggatgtgtc
    14341 atgtggttta tagccttgca gctggcgaga gacggtattg ctcatgcaca agccttgttc
    14401 agttaggcgc tatctgatgg aaaaataaaa cagaggcgct aagcttgcct ccagaggtcc
    14461 tgaattttgg gcaagtcaat taacacggag agactaacgt gaagttcaaa gttatcgccc
    14521 tggcggcatt aatgggtatt agcgggatgg cagcgcaggc taacgaattg ccggatggac
    14581 cgcatattgt cacctccggt acggcaagtg tggatgcggt gccagacatt gccactcttg
    14641 cgattgaagt taacgtggcc gcgaaggatg ccgctacagc caagaaacag gcagatgagc
    14701 gcgtcgcaca atacatttcc ttccttgaac tcaatcagat cgcgaaaaaa gatatcagct
    14761 cagcgaattt acgcacccag ccagattatg attatcagga tggtaaaagt atcctgaaag
    14821 gctaccgcgc tgtgagaacg gtggaagtca cgctccgtca gttagacaaa ctgaattcct
    14881 tgctggatgg cgcgctgaag gcgggtctta acgaaattcg ttctgtgtcg ctgggcgtgg
    14941 cgcagccgga tgcctataaa gacaaagcgc gtaaggcagc gattgataac gcgattcatc
    15001 aggcgcagga actggcgaac ggctttcatc gtaaactggg gccggtatat agcgtgcgct
    15061 accatgtttc caactatcag cccagcccaa tggtgcggat gatgaaagcc gatgccgcgc
    15121 cggtgtctgc ccaggaaact tacgagcagg ccgctattca gtttgatgat caggtcgatg
    15181 tggtcttcca gttagaacct gtggatcaac aacccgctaa aacacctgca gcacaataat
    15241 acttgtagcc cgacgtattc gtcgggctaa tgcctttacc cgatattgcc tcctttttct
    15301 gtaattttct gtaaaaccat gacgtaatca cctatctttg ctcgcggttt catcaaggat
    15361 aggctgactg aaacggttaa gcggacaagg atgcggggag gggggatgga catttttatt
    15421 tcgaaaaaga tgcgcaattt cattttatta gcgcaaacca ataatattgc ccgggcggca
    15481 gaaaaaatcc atatgacggc ttcgccattt ggtaaaagca ttgccgcgct ggaagaacaa
    15541 attggctata cgctatttac ccgcaaagat aataatatca gcctcaataa agccggtcag
    15601 gaactgtatc aaaaactgtt cccggtttat caacgacttt ctgcgattga taatgaaatc
    15661 cataactcag ggcgtcgttc acgagaaatt gtgattggca tagataatac ttatccgaca
    15721 attattttcg atcagttgat tagtctcgga gataagtatg aaggcgtgac cgcccagcca
    15781 gttgagttca gcgaaaacgg tgttatcgat aatctttttg accgtcagtt agattttatt
    15841 atctccccac agcatgtatc tgcacgagtt caggaactgg aaaacctgac tatcagtgaa
    15901 ttaccaccgc tgcgattggg ctttctcgtt tctcgccgtt atgaagagcg tcaagagcaa
    15961 gagctgcttc aggagctgcc ctggctacaa atgcgctttc aaaaccgggc taacttcgaa
    16021 gcaatgattg atgcgaatat gcgaccttgt gggatcaatc ccaccattat ttatcgtccc
    16081 tacagtttta tggccaaaat cagcgccgtg gagcgcggac attttctgac ggtgatcccg
    16141 cattttgcct ggcgactggt gaatccggca acgctgaaat attttgatgc gccgcacagg
    16201 ccgatgtata tgcaggaata tctttattca atcagaaatc atcgttatac cgccacgatg
    16261 cttcagcata ttgctgaaga tcgtgacggg acaaaccatt aacccagcat cgagccggtt
    16321 gcaattaaat tacggtgtaa gtcgaagacg tggctaagat cgtggtgaat atgtccgccg
    16381 ggcgcatttt ccagatagcg atgcagataa tcccgataca taggatgtgc acaattatca
    16441 atgatagtgc gggcgcgttg aagcggggaa agaccgcgca gatcggcgat cccttgttcg
    16501 gtgatgatca ctttgacgct gtgttcgctg tgatcaacat ggctgcacat tggcacgacg
    16561 gttgagatct tgccttcttt agcaatcgac ggagccataa agatcgacag atacgcgttg
    16621 cgttcaaaat caccgctgcc gccgatgccg ttcatcagat cgaccccggc tacgtgcgtt
    16681 gagttggcat gcccgtaaat atcaaactcc aggccgacgt tcagagcgat gacgcccaga
    16741 cgacggatga tttccgggtt attggaaatc tcctgcggac gcaacacaat gcggctggca
    16801 aagtaatcca tattgtcgta aatcttgcgc agggaatcgg ccgagattgt caggctggag
    16861 gcgctggccc cgctgatttt gccggtttcc agtaaatgca ccaccgattc ctgtagcact
    16921 tccgaataca tcatgaacgg aggaatttcc gggttttccc ccagacgcgc cattaccgca
    16981 ttattgatat tgcccacgcc actttgcagc ggcagaaatt ccggcggaat acgcccatgc
    17041 gccatttcct gcaataagaa cgtgaccacg ttatcggcaa tctgctggca catgggattt
    17101 tgcttatcca gcatattacc ggcgtcgggc aagttggttt ccacgacggc gacaatcttt
    17161 ttcggatcga tttgcacata gcgggtaccg acgcgatcca ttgcatggaa gatcgacacg
    17221 ctattgcgcc gcggtggcgc gccaggaatc acaatatctg ccagttctgc aacgcgcgga
    17281 tcgtgatagt gattaagttc aatgatcact ttcttcgccc gcagcagcca ggtcggcgca
    17341 ttaccgatcc cgctggttaa ccagactcga ccatccggtg ccagtgccga tgcttcaatg
    17401 acggcaacat caatttcgcc gaagaaaccg taattgacca tttgcgccac ttcgctcaaa
    17461 tgcaggtcaa cgaaactcac cgcgccctga ttgatctttt tacgtaaacc ggacgatgtt
    17521 tgatatggtg cacgccagga aacagcatcg gcgtcagaaa gtacatcgtc agcggcggcg
    17581 ctgattgacg cccccgtcag caggcgaatt tgatacggct ttttggcctc atgctgttcg
    17641 ttagctctgc gggcaatcgc ggtaggtagg gctttcggcg aacccgctgg ggtaaagccg
    17701 ctaaatgcca ccatgtcgtt atgctggata atttctgccg cttcatcggc ggtcatcctt
    17761 gtccactgag tttccatttt tactccttcg ttcgcaggga ttaatgacca acgaaattag
    17821 gtttacgttt ttcgaggaaa gcgttcatcc cttcctggta atcttcgctg tcataaaccg
    17881 cgcggcgcat cccctgaata cgttcaaatt catcggagtt catggtgtgt gcttcgccca
    17941 gtacacgcag ctcttctttg ataacggcaa tggctaacgg cgctttctca gagatgtggt
    18001 gcgccatttg taaggtgaaa tcttccagtt cttccacttc cacaacatgg ttgaggatgc
    18061 cgacagccag cgcgcgctgg gcggtgattg gcgaagcggt aaaaatcagc tctttgacaa
    18121 tgtggaagcc cgcgtcgcgg gtcaggttgt gaatgccgac caggttatac gggacgccga
    18181 ggtttacagg cgtcattgag aaggttgagg tactggcggc gataatcaga tcggaactca
    18241 tgatcatttc aaatgcgcca ccccaaacac taccttccac catcgaaata atcggtttcg
    18301 ggaatttttg gatcatgcgg gtgatttgac gcaatggatc atcataggag agcggatcgc
    18361 gaccgccaga cggcagttcg tgaatatcgt gacctgcgga gaagactttg gatccactcg
    18421 gcgcgcgcaa aatgatacag cgaatttccg gccggttgag atcgcttaac gcctgcataa
    18481 gatcatcaat aaagacttta cttaaggcat taagttttcg gccatagtta aactcaatga
    18541 ccgccacttt gttgatagtg gcaacgttca catactgata agacataaaa attcctttaa
    18601 tcaaaatatt gcgtctggat aaattcactg agctgccgca ggccggtgcg cggtgagagc
    18661 gtattgtttt tgaccgctaa aagcgtctgg cggtaatagc gatcgaaatc ttcattcgcg
    18721 aacaggtgat tcagtacttc ttcttcggtc tgcttacgca gccattccac cgattgttgt
    18781 tgccgcactt gttgtaaacg accactggca gttagcgcgg ttttgaagtc gatgatggcg
    18841 tgccagatct catcgattcc acgtttttcc agtgcgctac aagtcagaac ccgtggctgc
    18901 cattcgtcgt atttacgtcg cagaatatgc agggcactct cgtacatatg ccgggcaatg
    18961 gcgacattgg tatggttatc gccatcgtct ttgttgataa cgatcagatc agccacttcc
    19021 atcagccctt ttttaatgcc ctgcagatca tcgccgccac cggcaatttg caacgagata
    19081 aaacagtcca ccatgcgggc gacttctgtt tccgactgcc cgacgccaac cgtttcgaca
    19141 atcactacgt cataacccgc tgcttcgcat aacagcatta attcccgcgc tcgctgactg
    19201 gcaccgccca gatgaccgga ggatggtacc gggcgaataa acgccgcttc ggcacgcgcc
    19261 aggtcattca tgcgggtttt atccccgaga atgctaccgc cagtgaccgg gctgctggga
    19321 tcgaccgcaa taaccgcgac ctttaatccc tctcgaatca acaacatgcc aaaggcctca
    19381 agaaaggtac ttttccccgc gccgggggtg ccggtaacgc ccagtcgcag ggtgttaccg
    19441 cagtacggca taatggcatc aagcagctgc gtacttagtg cctgatgacg cgggtgacgg
    19501 ctttccacca gcgtcatggc ctgggcgagt gtggcacgct caccctgacg taagcggcga
    19561 atactttctg ccagcgtggc ttcattaatc atgatgctgg cttatcagat tcagtacgtc
    19621 gcgcacactg tcgagcatag gtgtacctgg accataaatc gccgccacgc cgcgctcttg
    19681 caggaaggcg taatcctgcg gcggaatgac gccacccgcg accacgcaga tatcttcgcg
    19741 tccccatttt ttcagcgctt cgaccagttc cgggatcagc gttttatgac cggcagccag
    19801 tgaggatgcg cccactacgt gaacgtcgtt ttctacggcc aggcgggcga tctcttcagg
    19861 tgtagagaac atcgggctta aatctacgtc gaaaccgaga tcggaatagg cgctggcgat
    19921 cactttcgcg ccgcgatcgt gtccatcctg gcccatctta gcgatcagaa tgcgcgggcg
    19981 acgaccattg tcggcaagga actgctccgt ttgcgcaaca atggcatcga actcggaggc
    20041 cgatttctca gactgatgat agctttgcgc aatcacgccg gtaacacact ggcttggcac
    20101 cagataacgg tcgaaagcga cttccagcgc atcggaaatt tcacccaggg tggcacgaac
    20161 gcgagcggca ttaacagcgg cagccagcag gttttcgtta tgctgtgcgg cgtgagtcag
    20221 ggcgttcaac gcggcggtta cggcggcatc atcacgggtg gcgcgaatgc gttccagcga
    20281 agcaatttgc tcgttacgca ccatcacgtt gtcgatctca agtacatcgg tttcgtcttc
    20341 gtgatccagt ttgtacttgt tgacaccaac gatgacacgc ttgccctggt cgatcagcga
    20401 ctgttcgcgc gctgaggcct cttcgatcat tcgttttggc agacctgctt cgatcgcttt
    20461 cgccatgcca ccggcttcgt cgatctgttg gataatagct ctggcttgtt tgacgatttg
    20521 atcggtcagc gactcaatgt aataggatcc ggccagtgga tcgacggtgc ggcagagttc
    20581 tgattcttcc tggatgatga tctgggtgtt gcgggcaatg cgtgctgaga aatcggtagg
    20641 caaaccaagc gcttcgtcaa aggcgttggt atgcagtgac tgagtaccgc ccagcgtcgc
    20701 agccagtgct tcaatggtgg tgcggataac gttgttatac ggatcctgtt cagtcaggct
    20761 ccagcctgag gtctggcagt gggtacgcag cgccagtgat ttcggatcct gtgcgccaaa
    20821 tccactgacc gcttcgctcc ataaataacg tgccgcacgc aacatggcga cgttcataaa
    20881 cagatccatg ccgataccga agaagaacga caggcgagga gcgaagtcat caattttcag
    20941 tccggcagag attgctgctt tgatgtactc aatcccatca gcgagcgtaa atgctacctg
    21001 ctgcacgcag ttggcacccg cttcccccat gtggtaaccg ctgatactga tggtattaaa
    21061 tcgcggcatg ttgccggaac accaggcgat gatgtcggcg ataatgcgca ttgacggttt
    21121 tggtgggtaa atataggtgt tgcggcagag gtactctttg aggatatcgt tctgaatggt
    21181 gccggtcagt ttatcaggtg taacaccttg ctcttctgcg gcgacgatat aaaacgccag
    21241 tactggtagc actgcgccat tcatggtcat cgaaaccgac attttatcca gcgggatctg
    21301 gtcgaacagg actttcatat cttccacggt gtcgatagcg acgcccgctt tgccgacgtc
    21361 gcccgccacg cgcgggttat cggagtcgta gccacggtgg gtggcaaggt caaacgcaac
    21421 ggaaagacct ttttgcccgg cggccaggtt acggcgataa aaagcgttgg actcttttgc
    21481 tgttgaaaaa ccagcatact gacggatggt ccacggttgg gcggtataca tagtggcacg
    21541 cgggccacga acgtagggcg gcaaaccagg aagggtacct gtcacctcca gattatcgag
    21601 atcggcttcg gtatacagcg gcttgatggc gatcccttcc gcggtttgct gaaccagcga
    21661 gtcgacagtt ttctcccgac ggctcaattc cttgttggca agctgttgcc actcctgcac
    21721 gttagacatt atttgctccg taaaacgcaa tcaaaatgag ggtatttgcg cctacggtga
    21781 gctttttacg tgggtttgtc gccagcaaaa aaaatgcatt cagacgtcgt tttttgccgc
    21841 ctgagatttg tctattttgt gatgtgattc aaggaataca aaaagaagtg aggacaacgc
    21901 ctgatatgtg ctatcaggca ttatttgatg gattaatcct gacgaaggac tttgtgacca
    21961 taatcgagta acgcatcagt gactttacgc atcatgcggc tttcaggagc aaagcggtgc
    22021 cagtagagca tccgtcgttg aaatagccca ggcgttaagt caatcagttc accgctggcc
    22081 agctctttct cgatttgcag gtgcgggatc atacagcagg tggtgccctg gcgagcaagt
    22141 tgtacgaacg cttctgaaga attaacgata tggcagggca cgctgcctgg aggcagatcg
    22201 aagttttgct gcaaaaaggc ctggtgcata tcgtcaagat ggtcaaacgc gaccactggc
    22261 gctttcagta atgccgaacg cgttacgccg ttagggaaat atttttcggc aaagggtttt
    22321 gagctgacga acagatagtc gagcgcacca agtttatcga caagacaact cggcagcgcc
    22381 tgatgttgaa tactcaccgc gccgaccact tcgccgcggc gcagacgttc ctgagtgcgg
    22441 gtttcatctt ctacctgcaa gttgaggcgg ataggcgaat cagccaacac aggagccagt
    22501 gcaggaagca accacgtcgc cagactgtcg gcgttgaccg ccagtgaaag cagcagcgga
    22561 gtcgaaccgg tttgttcatc gcccagccac tcttcttcca gcaactccac ctggcgcagc
    22621 agtgccagca gtttttgccc ttgttccgtc gggcgcggcg gtacggtacg caccaacagc
    22681 ggctgcccga acatattttc cagttgctta atgcgctgtg agacggctga ttgtgtaatg
    22741 cacagctttt gtgccgcgcg ctcaaatcct cgttcacgta tcaccgcatc cagtgcctgt
    22801 aatgttctgt agtccgggcg tttcattgct gttgtctgct cctggtaaat gctttttcct
    22861 gcactatgac acaattttgt gtcaggttgc atataccgaa gccgtcggcg ggtaacattt
    22921 gtattcgtgg ttaaacgcgc agcttaaaag atcagggatt tgcgctaatg catttcgctc
    22981 agcgagttcg cgccctggtt gtgttgaatg gcgtggcgtt attgcctctg ttcgcaccgt
    23041 ggagcggcat acccagaccg ttgtatgctt tatttgcggg gcgaaagggg atgcccgcca
    23101 ttgcgcgata ttttatggat gagttaacca cgcggcttgc caacggggtc tgaatcgctt
    23161 tttttgtata taatgcgtgt gaaatttcat accacaggcg aaacgatcat gacgcaggat
    23221 gaattgaaaa aagcagtagg atgggcggca cttcagtatg ttcagcccgg caccattgtt
    23281 ggtgtaggta caggttccac cgccgcacac tttattgacg cgctcggtac aatgaaaggc
    23341 cagattgaag gggccgtttc cagttcagat gcttccactg aaaaactgaa aagcctcggc
    23401 attcacgttt ttgatctcaa cgaagtcgac agccttggca tctacgttga tggcgcagat
    23461 gaaatcaacg gccacatgca aatgatcaaa ggcggcggcg cggcgctgac ccgtgaaaaa
    23521 atcattgctt cggttgcaga aaaatttatc tgtattgcag acgcttccaa gcaggttgat
    23581 attctgggta aattcccgct gccagtagaa gttatcccga tggcacgtag tgcagtggcg
    23641 cgccagctgg tgaaactggg cggtcgtccg gaataccgtc agggcgtggt gaccgataat
    23701 ggcaacgtga tcctcgacgt ccacggcatg gaaatccttg acccgatagc gatggaaaac
    23761 gccataaatg cgattcctgg cgtggtgact gttggcttgt ttgctaaccg tggcgcggac
    23821 gttgcgctga ttggcacacc tgacggtgtc aaaaccattg tgaaatgatc tgacggggga
    23881 acctcccccg ttaaaaaaat tctcttcatt aaatttggtg acatgtgtca cgtttttacc
    23941 tggctattgt cgattgctct aaataaatct tctaaaccag catattcatc caagaattac
    24001 ctttgcgtga tatttcctca acatcgcgac gcaaacgttc atattgccgc aatattattt
    24061 tttgatatgt tgaaaggcgg acgcaaatcc gcacacaaca tttcaaaaga caggattggg
    24121 taaatggcaa aggtatcgct ggagaaagac aagattaagt ttctgctggt agaaggcgtg
    24181 caccaaaagg cgctggaaag ccttcgtgca gctggttaca ccaacatcga atttcacaaa
    24241 ggcgcgctgg atgatgaaca attaaaagaa tccatccgcg atgcccactt catcggcctg
    24301 cgatcccgta cccatctgac tgaagacgtg atcaacgccg cagaaaaact ggtcgctatt
    24361 ggctgtttct gtatcggaac aaaccaggtt gatctggatg cggcggcaaa gcgcgggatc
    24421 ccggtattta acgcaccgtt ctcaaatacg cgctctgttg cggagctggt gattggcgaa
    24481 ctgctgctgc tattgcgcgg cgtgccggaa gccaatgcta aagcgcaccg tggcgtgtgg
    24541 aacaaactgg cggcgggttc ttttgaagcg cgcggcaaaa agctgggtat catcggctac
    24601 ggtcatattg gtacgcaatt gggcattctg gctgaatcgc tgggaatgta tgtttacttt
    24661 tatgatattg aaaataaact gccgctgggc aacgccactc aggtacagca tctttctgac
    24721 ctgctgaata tgagcgatgt ggtgagtctg catgtaccag agaatccgtc caccaaaaat
    24781 atgatgggcg cgaaagaaat ttcactaatg aagcccggct cgctgctgat taatgcttcg
    24841 cgcggtactg tggtggatat tccggcgctg tgtgatgcgc tggcgagcaa acatctggcg
    24901 ggggcggcaa tcgacgtatt cccgacggaa ccggcgacca atagcgatcc atttacctct
    24961 ccgctgtgtg aattcgacaa cgtccttctg acgccacaca ttggcggttc gactcaggaa
    25021 gcgcaggaga atatcggcct agaagttgcg ggtaaattga tcaagtattc tgacaatggc
    25081 tcaacgctct ctgcggtgaa cttcccggaa gtctcgctgc cactgcatgg tggtcgtcgt
    25141 ctgatgcaca tccacgaaaa ccgtccgggc gtgctaactg cgctgaacaa aatcttcgcc
    25201 gagcagggcg tcaacatcgc cgcgcaatat ttacaaactt ccgcccagat gggttatgta
    25261 gttattgata ttgaagctga cgaagacgtt gccgaaaaag cgctgcaggc aatgaaagct
    25321 attccgggta ccattcgcgc ccgtctgctg tactaattcc ccttctctga aaattaacgg
    25381 gcaggtcact gacttgcccg tttttttatc ccttctccac accgttgccg cgttgcgcat
    25441 tctactttgc gagtctgctc cagaaaataa atcaaagtca gatcaatgcg ttgtgtttat
    25501 ttaatgggga tttacgatgg cgggacagca tggggctgta acggtaaagc cctcaccgaa
    25561 gcgagggctt aaaggagaag ggttatgatg cgacttgtca tcatactgat tgtactgtta
    25621 ctgataagtt tcggagctta ttaacagtca gtctcagggg aggagtcatc ctcccttacc
    25681 cttactcact aaattaggtc aaagaatcaa cgatgtcaat cagggcgatg cggggtgcat
    25741 cgccctgaac cgaacttttt gatagccact tcatcgccca cgtttactgc cgtaatagat
    25801 cctttttccc aaaaattatc tctgttttgc atggggtttt tcgagcacgc tcgaagaaaa
    25861 gatttaaagg tagatcaatg cgttgtggtt atttaaagag agcttacgat agctgggcag
    25921 catggggctg taacggtaaa gccctcaccg aagcgagggc ttgaaggaga agggttatga
    25981 tgcgacttgc catcatactg attgtactgt tactcataag tttcccagct tattaacagt
    26041 cagtcacagg ggaggagcaa tcctccctta cccttactca ctaaattagg ttaaagaatc
    26101 aacgatgtca atcagggcga tgcaggtgta tcgcccttac cactcccaga ctttcgacgg
    26161 tgtaaccacc gcaggaagag ggatatccca ctcttcaacg gggagttttt ccaccaactg
    26221 acaatcatgc gcataaccca ccggttgcgt tttatagtgc tgccagtttt gtaaggtccg
    26281 atcataaaaa ccaccgccca ttcccaggcg ctgaccgtac tcatcaaagg cgaccagcgg
    26341 tgtgatcagc acgtctaatc gggaaagggg tagcacgtca cgcacatcca gttttggctc
    26401 atggatcttc aacctgttca tcaccagttc gctttgcgga tggtaattca ggaacagcaa
    26461 attaccggca ctaaagggat gcaaaactgg aagatatacg cgcttaccgg cgcgccagag
    26521 ttgttctatt agcggctggg tgtcgagttc accatcaaaa gagaggaata cagcgaccgt
    26581 atgcgccatc accaccgggg gataagtcat catccgggta gcggcttgtt gacccatttc
    26641 ctgctgttgt tccggcgtta acgcacgacg acgttgccga atcattttgc ggatttcttg
    26701 tcgggataat gtcagtggga gttctggtag ttgcgtcatg gctggtgcca gataagaagg
    26761 gaatctccga gatgccgccg caggctgtaa cccttgaacc cttggttcaa ggtgaatgtg
    26821 tcgtcgcagt tttaaggctt ctcggacgga ccgagcatgc tcaccaaccg cggagcgcca
    26881 cattcttgtg gtatgaaata tcggctcagg ggactggccc gcttgcgaac atctcagaga
    26941 aattttgtct tcacggttac tctaccacag taaaccgaaa agtgtcattc aaagttttgg
    27001 ttagtttttt cggtgatgcg accttgttca agtaacgctt gttctatggt ctgctgcagc
    27061 atccgaatac gctgttccat acttgccgcg tagtcacgag tcttcgcttt ttcttgcgct
    27121 aactcatagc tgatattcaa tgcggcaatg aagaccaact gttcagtatt tgtgactcta
    27181 gtgcgttctt tcagatcttg caaccgttgg ttcagatcgt ccgctgcctg attcaacgca
    27241 tccctttggt caggcgggca gttcacacgc agtgaacggc caaaaatttg gatatcgacg
    27301 ggttgtgcag acatgccacc ttcctgctga ttgactgcgc tgccttcgtc tttagaccca
    27361 tggtctgcga aggggcgaca ctatagctac cctgatgaga agagacaagc ccttttctgg
    27421 tccaccaggg cccaaagtgg tagcatatca tgaatattcc tccctttgac gacgaatgct
    27481 tatgtctata cagaacgaaa tgcctggtta caacgaaatg aaccagtatc tgaaccaaca
    27541 agggacgggt ctgacccctg ctgagatgca tggtttaatc agtgggatga tatgtggcgg
    27601 taacgatgac agctcatggc taccgctact tcacgacctg acgaacgaag gcatggcttt
    27661 cggtcatgag ctggcacagg cactgcgtaa aatgcactct gccaccagcg atgccctgca
    27721 ggatgacggc ttcctttttc agctttatct gcctgatggc gatgatgtca gcgttttcga
    27781 tcgggctgat gcattggcag gttgggtcaa tcacttcctg cttggtcttg gcgttacgca
    27841 accgaagctg gataaagtga ccggcgaaac cggtgaagct atcgacgatc tgcgtaacat
    27901 tgcgcaactg ggttacgacg aagacgaaga tcaggaagag cttgaaatgt cgcttgaaga
    27961 gatcatcgaa tacgttcgtg ttgccgcgct gttatgccac gacaccttta ctcatccgca
    28021 accgaccgcg ccagaagtac aaaaaccgac tctacactaa aaacaaaaaa cgtaaggaga
    28081 gtgttatgag tgagatatcc cggcaagagt ttcagcgtcg ccgtcaggcc ctggtggagc
    28141 aaatgcaacc cggcagcgcc gcgctgattt ttgctgcacc agaagtaaca cgtagcgccg
    28201 acagcgaata cccctatcgt cagaacagtg acttctggta cttcaccggc tttaacgaac
    28261 cggaagcggt gctggtgctg attaaaagcg atgacactca taaccacagc gttctgttta
    28321 accgcgttcg cgacctgacg gcggagatct ggtttggccg tcgtttaggt caggatgccg
    28381 cgccagagaa actgggcgtt gaccgcgcac tggcattcag cgaaattaat cagcaacttt
    28441 atcaattact taatggtctg gatgtggttt accacgccca gggcgaatat acctatgctg
    28501 atgaaatcgt gaacagcgcg ctggaaaagc tgcgtaaagg ttcgcgacaa aatctcaccg
    28561 cgccggcaac gatgatcgac tggcgtccca ttgttcatga aatgcgcctg ttcaaatcgc
    28621 cagaagagat tgccgtactt cgccgcgcgg gtgaaatcac cgccctggcg catacccggg
    28681 cgatggaaaa atgccgtccg ggaatgttcg agtaccacct ggaaggcgaa attcaccacg
    28741 aatttaaccg ccacggcgcg cgctatcctt cctataacac cattgtcggc agcggtgaaa
    28801 acggctgcat tttgcactac accgaaaacg agtgtgaact gcgtgacggt gacctggtgt
    28861 tgattgacgc gggttgtgaa tacaaaggtt acgctggcga tattacccgc accttcccgg
    28921 tcaacggcaa attcactcag gcccagcgtg aaatctacga cattgtgctg gagtctctcg
    28981 aaaccagctt gcgcctgtat cgtccgggaa cttccatgca ggaagtcacc ggtgaagtgg
    29041 tgcgcatcat ggttagcggc ctggtaaaac tcggcattct gaaaggtgat gttgatgaac
    29101 tgatcgctca gaacgcccat cgtcctttct ttatgcatgg ccttagccac tggttagggc
    29161 tagatgtcca tgacgttggc gtttatggtc aggatcgctc gcgcattctg gaaccgggca
    29221 tggtactgac cgtagagcca gggctgtata ttgcgccgga tgcagaagtg ccagaacaat
    29281 atcgcggtat cggcattcgt attgaagacg acattgtgat taccgaaacc ggtaacgaaa
    29341 acctcaccgc cagcgtggtg aaaaagccgg aagaaatcga agcgttgatg gcagctgcga
    29401 gaaagcaatg agcgtaatca tcgtcggtgg cggcatggcg ggcgcgacgc tggcgctggc
    29461 tatttcccgg ttaagtcacg gggcgctgcc ggtacatttg attgaagcga ccgcgccaga
    29521 gtcacatgct catccgggct ttgatggacg agctattgcg ctagcggcgg ggacctgtca
    29581 gcaactggcg cgcatcggcg tctggcaatc tctggctgat tgcgcaaccg ccatcactac
    29641 cgtgcatgtc agcgatcgtg gtcatgctgg attcgtcacc ctcgccgcag aagattacca
    29701 actggcggcg ctggggcagg ttgtcgaatt gcataatgtc gggcaacggc tgtttgcatt
    29761 gctgcgaaaa gcacctggcg taatgctgca ttgccccgat cgcgtagcta acgttgcccg
    29821 tactcagagt cacgttgaag tgacgctgga gagcggcgag atgctgacgg gccgcgtgct
    29881 gatagccgct gatggcaccc attcagcgtt agccaccgcg tgcggcgttg attggcagca
    29941 ggagccttac gaacaactgg ccgtgattgc caacgttgcg acttccgttg cgcacgaagg
    30001 ccgcgctttt gaacgcttca cgcaacatgg cccgctggcg atgttgccga tgtctgacgg
    30061 acgctgttcg ctggtctggt gtcatccact ggaacggcgc gaagaggtgc tgtcgtggag
    30121 cgacgagaag ttttgccgtg aactccagtc ggcctttggc tggcgacttg gaaaaattac
    30181 ccacgccggt aaacgcagtg cttatccgct ggcgttaacc cgcgccgcca gagctattac
    30241 ccatcgtacc gtgctggtgg gcaatgcggc gcaaactctg cacccgattg ccgggcaagg
    30301 gtttaacctc ggtatgcgag atgtcatgag ccttgcggaa accctaactc aggcgcatga
    30361 gcgcggagaa gacatcggtg attacggcat attgtgccgc tatcaacagc gtcgacagag
    30421 cgatcgcgaa gcaaccatcg gcgtcacgga cagcctggta catctttttg ccaaccgttg
    30481 ggcaccgctg gttgtcgggc gcaacatcgg actgatgacg atggaattat tcaccccggc
    30541 acgcgatgtg ctggcgcagc gcaccctcgg ttgggtggcg cgttgaaggc ggttttaagg
    30601 aataaacaat gcaaagtgtt gatgtagcca ttgttggtgg cggcatggtg gggctggcgg
    30661 ttgcctgcgg cttacagggg agcggcttac gcgttgccgt actggagcag cgcgtaccgg
    30721 aacctctggc ggcggatgca ccaccacaac tgcgcgtttc ggctatcaat gccgccagcg
    30781 aaaaattact cacccgtctt ggcgtctggc aggacattct ctctcgtagg gccagttgtt
    30841 atcacggtat ggaagtgtgg gacaaagaca gttttggtca catttcgttt gacgatcaaa
    30901 gcatgggcta tagccatctt gggcatgtcg ttgaaaactc agtgattcac tacgcgttgt
    30961 ggaacaaagc gcagcagtcg tcagatatca ctctgttggc ccccgcagaa ttacagcagg
    31021 tcgcctgggg agaaaatgaa actttcctga cgctgaaaga tggcagcatg ttaacggcgc
    31081 gtctggtgat tggcgcggac ggtgctaatt cctggttgcg caacaaagca gatattccgc
    31141 tgactttctg ggattatcag catcacgcgc tggtagcgac cattcgcacg gaagaaccgc
    31201 atgatgcggt ggcgcggcag gttttccatg gcgaaggcat tctggccttt ttaccgctta
    31261 gcgatccgca tctttgctca attgtctggt cactgtcgcc agaggaagcg cagcggatgc
    31321 agcaggcaag tgaagatgaa tttaatcgcg cgttaaatat cgcttttgat aatcgcctgg
    31381 gcttatgcaa ggttgagagc gcgcgtcagg tgttcccact gacggggcgt tatgcgcgcc
    31441 agtttgccgc gcaccgtctg gtgctggtgg gcgacgccgc gcataccatt cacccgctgg
    31501 cggggcaggg agtaaatctc ggctttatgg atgctgcaga gctgattgct gaactgaaac
    31561 ggttgcatcg tcaggggaaa gacatcgggc agtacattta cctgcgccgg tatgagcgta
    31621 gccgcaagca cagtgcggcg ctgatgctgg ctggtatgca gggattccgc gatctgtttt
    31681 ccggtaccaa tccggcgaaa aaactgctgc gtgatattgg tttgaaactg gccgacacgc
    31741 ttcctggcgt taaaccacaa cttatccgcc aggcaatggg attaaacgat ttgcctgaat
    31801 ggctgcgtta aaaatttctc ttctgctgtt tatttgatac ccatcacact ttcatctccc
    31861 ggttttttcg ccgggagatt ttcctcattt gaaataaact aatttcacct ccgttttcgc
    31921 attatatttt ctaatgtcat tattttttga tttagtgttt tttgacattt ttttagctct
    31981 taatattgcc ttattcaaat tagctttcta atggcatcat ctttatatag aaactggtgt
    32041 gttttttggt tttttattct gtcgcgattt tggcgttttt taaccataag ctaatgtgat
    32101 gatcaatttt accttatggt taacagtctg tttcggtggt aagttcaggc aaaagagaac
    32161 gattgcgttg gggaccggga gtggctccga tgctgggttt cgtggtgata atttcaccat
    32221 gaaaagttgt cagccccgct tattcaatga ggacaagatg gcacaacaga ctcctttgta
    32281 cgaacaacac acgctttgcg gcgctcgcat ggtggatttc cacggctgga tgatgccgct
    32341 gcattacggt tcgcaaatcg acgaacatca tgcggtacgt accgatgccg gaatgtttga
    32401 tgtgtcacat atgaccatcg tcgatctccg cggcagccgc acccgggagt ttctgcgtta
    32461 tctgctggcg aacgatgtgg cgaagctcac caaaagcggc aaagcccttt actcggggat
    32521 gttgaatgcc tctggcggtg tgatagatga cctcatcgtc tactacttta ctgaagattt
    32581 cttccgcctc gttgttaact ccgccacccg cgaaaaagac ctctcctgga ttacccaaca
    32641 cgctgaacct ttcggcatcg aaattaccgt tcgtgatgac ctttccatga ttgccgtgca
    32701 agggccgaat gcgcaggcaa aagctgccac actgtttaat gacgcccagc gtcaggcggt
    32761 ggaagggatg aaaccgttct ttggcgtgca ggcgggcgat ctgtttattg ccaccactgg
    32821 ttataccggt gaagcgggct atgaaattgc gctgcccaat gaaaaagcgg ccgatttctg
    32881 gcgtgcgctg gtggaagcgg gtgttaagcc atgtggcttg ggcgcgcgtg acacgctgcg
    32941 tctggaagcg ggcatgaatc tttatggtca ggagatggac gaaactattt ctcctttagc
    33001 cgccaacatg ggctggacta tcgcctggga accggcagat cgtgacttta tcggtcgtga
    33061 agccctggaa gtgcagcgtg agcatggtac agaaaaactg gttggtctgg tgatgaccga
    33121 aaaaggcgtg ctgcgtaatg aactgccggt acgttttacc gatgcgcagg gcaaccagca
    33181 tgaaggcatt atcaccagcg ggactttctc cccgacgctg ggttacagca ttgcgctggc
    33241 acgcgtgccg gaaggtattg gcgaaacggc gattgtgcaa attcgcaacc gtgaaatgcc
    33301 ggttaaagtg acgaaacctg tttttgtgcg taacggtaaa gccgtcgcgt gatttacttt
    33361 tttggagatt gattgatgag caacgtacca gcagaactga aatacagcaa agaacacgaa
    33421 tggctgcgta aagaagccga cggcacttac accgttggta taaccgaaca tgcccaggag
    33481 ctgttaggcg atatggtgtt tgttgacctg ccggaagtgg gcgcaacggt tagcgcgggc
    33541 gatgactgcg cggttgccga atcagtaaaa gcggcgtcag acatttatgc gccagtaagc
    33601 ggtgaaatcg tggcggtaaa cgacgcactg agcgattccc cggaactggt gaacagcgaa
    33661 ccgtatgcag gtggctggat ctttaaaatc aaagccagcg atgaaagcga actggaatca
    33721 ctgctggatg cgaccgcata cgaagcattg ttagaagacg agtaacggct ttattcctct
    33781 tctgcgggag aggatcaggg tgaggaaaat ttatgcctca ccctcactct cttcgtaagg
    33841 agagaggttc acaattcact gcacgtttca ggaaccatcg ctcatgacac agacgttaag
    33901 ccagcttgaa aacagcggcg cttttattga acgccatatc ggaccggacg ccgcgcaaca
    33961 gcaagaaatg ctgaatgccg ttggtgcaca atcgttaaac gcgctgaccg gccagattgt
    34021 gccgaaagat attcagcttg cgaccccgcc gcaggttggc gcaccggcga ccgaatacgc
    34081 cgcactggca gaactcaagg ctattgccag tcgcaataaa cgcttcacgt cttacatcgg
    34141 catgggttac accgccgtgc agctaccgcc ggttatcctg cgtaacatgc tggaaaatcc
    34201 gggctggtat accgcgtaca ctccgtatca acctgaagtc tcccagggcc gccttgaagc
    34261 actgctcaac ttccagcagg taacgctgga tttgactgga ctggatatgg cctctgcttc
    34321 tcttctggac gaggccaccg ctgccgccga agcaatggcg atggcgaaac gcgtcagcaa
    34381 actgaaaaat gccaaccgct tcttcgtggc ttccgatgtg catccgcaaa cgctggatgt
    34441 ggtccgtact cgtgccgaaa cctttggttt tgaagtgatt gtcgatgacg cgcaaaaagt
    34501 gctcgaccat caggacgtct tcggcgtgct gttacagcag gttggcacaa ccggtgaaat
    34561 ccacgactac accgcgctta ttagcgaact gaaatcacgc aaaattgtgg tcagtgtcgc
    34621 cgccgatatt atggcgctgg tgctgttaac tgcgccgggt aaacagggcg cggatattgt
    34681 ttttggttcg gcgcaacgct tcggcgtgcc gatgggctac ggtggcccac acgcggcctt
    34741 ctttgcggcg aaagatgaat acaaacgctc aatgccgggc cgtattatcg gtgtatcgaa
    34801 agatgcagct ggcaataccg cgctgcgcat ggcgatgcag actcgcgagc aacatattcg
    34861 ccgtgagaaa gcgaactcca acatttgtac ttcccaggtg ctgctggcaa acatcgccag
    34921 cctgtatgcc gtttatcacg gcccgattgg cctgaaacgt atcgctaacc gcattcaccg
    34981 tctgaccgat atcctggcgg cgggcctgca acaaaaaggt ctgaaactgc gccatgcgca
    35041 ctatttcgac accttgtgtg tggaagtggc cgacaaagcg ggcgtactga cgcgtgccga
    35101 agcggctgaa atcaacctgc gtagcgatat tctgaacgcg gttgggatca cccttgatga
    35161 aacaaccacg cgtgaaaacg taatgcagct tttcaacgtg ctgctgggcg ataaccacgg
    35221 cctggacatc gacacgctgg acaaagacgt ggctcacgac agccgctcta tccagcctgc
    35281 gatgctgcgc gacgacgaaa tcctcaccca tccggtgttt aatcgctacc acagcgaaac
    35341 cgaaatgatg cgctatatgc actcgctgga gcgtaaagat ctggcgctga atcaggcgat
    35401 gatcccgctg ggttcctgca ccatgaaact gaacgccgcc gccgagatga tcccaatcac
    35461 ctggccggaa tttgccgaac tgcacccgtt ttgtccgccg gaacaggctg aaggttatca
    35521 gcagatgatt gcgcagctgg ctgactggct ggtgaaactg accggttacg acgccgtttg
    35581 tatgcagccg aactccggcg cacagggcga atacgcgggc ctgctggcga ttcgtcatta
    35641 tcatgaaagc cgcaacgaag ggcatcgcga tatctgcctg atcccggctt ctgcgcacgg
    35701 aactaacccc gcttctgcac atatggcagg aatgcaggtg gtggttgtgg cgtgtgataa
    35761 aaacggcaac atcgatctga ctgatctgcg cgcgaaagcg gaacaggcgg gcgataacct
    35821 ctcctgtatc atggtgactt atccttctac ccacggcgtg tatgaagaaa cgatccgtga
    35881 agtgtgtgaa gtcgtgcatc agttcggcgg tcaggtttac cttgatggcg cgaacatgaa
    35941 cgcccaggtt ggcatcacct cgccgggctt tattggcgcg gacgtttcgc acctcaacct
    36001 gcataaaact ttctgcattc cgcacggcgg tggtggtccg ggtatgggac cgatcggtgt
    36061 gaaagcgcat ctggcaccgt ttgtaccggg tcatagcgtg gtgcaaatcg aaggcatgtt
    36121 aacccgtcag ggcgcagttt ctgcggcacc gttcggtagc gcctctatcc tgccaatcag
    36181 ctggatgtac atccgcatga tgggcgcaga agggctgaaa aaagcaagcc aggtggcaat
    36241 cctcaacgcc aactatattg ccagccgcct gcaggatgcc ttcccggtgc tgtataccgg
    36301 tcgcgacggt cgcgtggcgc acgaatgtat tctcgatatt cgcccgctga aagaagaaac
    36361 cggcatcagc gagctggata ttgccaagcg cctgatcgac tacggtttcc acgcgccgac
    36421 catgtcgttc ccggtggcgg gtacgctgat ggttgaaccg actgaatctg aaagcaaagt
    36481 ggaactggat cgctttatcg acgcgatgct ggctatccgc gcagaaatcg accaggttaa
    36541 agccggtgtc tggccgctgg aagataaccc gctggtgaac gcgccgcaca ttcagaacga
    36601 actggtcgcc gagtgggcgc atccgtacag ccgtgaggtt gcagtattcc cggcaggtgt
    36661 ggcagacaaa tactggccga cggtgaaacg tctggatgat gtttacggcg accgtaacct
    36721 gttctgctcc tgcgtaccga ttagcgaata ccagtaattc actgattcga ctattttcta
    36781 aaggcgcttc ggcgcctttt tagtcagatg acaaagtaca aaagtgctca gacagtcccc
    36841 tcgccccttt ggggagaggg tgaggggaac aggccggcac tggcgcgaat atttaccctc
    36901 accccggccc tctccctgaa agggcgaggg ggaaaagcgt gccaacattg aagattgagc
    36961 cagtttgtta gcaatctcaa agatacgtca acgaatgaat ttttctcgga aaaacaaatg
    37021 gttatagcac ttgtgaccgg tggcagtcgc ggcatcgggc gggcaactgc attactgttg
    37081 gcgcaagaag ggtatacggt ggcggttaat tatcagcaaa acctccacgc ggcgcaggaa
    37141 gtgatgaact taataacgca agccggtggc aaagcattcg tgctccaggc ggatatcagc
    37201 gacgaaaacc aggtcgttgc gatgtttaca gcaatcgatc agcacgatga accgctagca
    37261 gcgctggtca ataacgccgg gatcttgttt acccagtgca ccgttgaaaa ccttaccgca
    37321 gagcgaatca accgagtact ttccaccaac gtgacgggat attttctctg ctgccgcgag
    37381 gcggtaaaac gcatggcgct taaaaatggt ggcagtggcg gcgctatcgt caatgtctct
    37441 tcggtggcct cacggttggg ttcgccaggg gaatatgttg attacgcggc atcgaaaggg
    37501 gcgattgata cgttaaccac cggactatcg ctggaagtcg ccgcgcaggg gatccgcgtt
    37561 aactgcgtgc ggccagggtt tatttatacc gaaatgcacg ccagcggcgg cgagcctgga
    37621 cgcgtcgatc gcgttaagtc gaacatcccc atgcagcgtg gtggacaggc agaagaggtc
    37681 gcgcaggcca ttgtctggct actaagtgat aaagcctctt acgtcacggg aagttttatc
    37741 gatttggcgg gcgggaaata aaacagggaa gttgtctgac cggatgcaac aagtattgca
    37801 tccggtactt catcgactta aagcttctcg ccgttgctgg caatcacctc ttggtaccag
    37861 ttaaagctct tcttacgtga acgcgacata tcgccagcac cgtcgtcatg tttattcaca
    37921 tagataaagc cgtagcgttt gctgtactgc ccggtggtga acgatacgca gtcgatgcag
    37981 ccccacggtg tgtagcccat cagatccacg ccatcgtaag tcaccgcttt tttcatctct
    38041 tcgatatggg cgcgcaggta gtcaatgcgg tagtcgtcgt tgatgctgcc atcttcttcc
    38101 actttgtcgt aagcgccaaa accgttttcg acaataaaca gcggcctctg ataacgctca
    38161 tacagttcgc aaagggcata gcgcagacct actggatcaa tctgccagcc ccagtcagac
    38221 gctttaacat acgggttcgg tacgctgcct tcgaaaccag agatcgcatc gccggtgccg
    38281 ccttcggcct tcactgcgtt ggtcatgtaa tagctgaaac caagataatc gcaggtgcct
    38341 tcacgcagta catcgagatc gccgtcttcc attttgatgt taaatccgcg acgctcccac
    38401 tcgttcaaca cataggacgg gtaatagccg cgtagctgca catcggtaaa gacgtagcgt
    38461 tcgcgcatcg actcctgagc gaacatcaca tcgtccgggt tacagcagta gggatagagc
    38521 ggcaccatcg ccagcataca gccgactttc atctccgggt taatgcgacg cgcagctttc
    38581 accgccaggg cgctggcgac aaactggtga tgcagcacct ggtacatcgt ctcttccggg
    38641 ttgtcatgct cggtataaac cacgccggag cagcagtaac cgaacagcgg tgtacgccag
    38701 ttacgctggt tgttaatttc gttgaaggtc atccagtatt tgactttatg cttatagcgt
    38761 tcaaacaccg cttccgcgaa acggacaaag aaatcaacca ctttacggtt ggtccagcta
    38821 ccgtattgct gcaccagatg cagcggcatt tcaaagtggg agagggtgat caccggttcg
    38881 atgttgtatt tcagcagttc atcgaacata tcatcgtaga acttcagccc ttcttcgttt
    38941 ggctgagctt catcaccttt tggaaaaata cgcgtccagg cgatggaagt ccgaaaacat
    39001 ttgaagccca tttcggcaaa tagcttgatg tcttccttgt agtgaccata aaaatcaacg
    39061 gcttcatggt ttggatagta tttgcctggc aagacttctt tggtgatttc gcgcggcacg
    39121 ccgtgtgcgc caccggtcag aacgtcacaa atgctcggcc ctttgccgcc tttgttccag
    39181 ctgccttcga cctgatgagc ggcaactgcg ccgccccata agaaatcttt cggtaaggtg
    39241 agttttttca ctatcatagg ctccagatga gtcaattctt acaggaaata gtaccatgca
    39301 gccaaacgac atcacttttt ttcaacgttt ccaggatgac attctggctg ggcgtaaaac
    39361 catcaccatc cgcgacgagt ctgaatcgca cttcaaaacg ggtgatgtgc ttcgtgtcgg
    39421 gcgttttgaa gatgacggtt atttttgcac gattgaagtc accgcaacct caaccgtaag
    39481 gctggatacg ctgactgaaa aacatgcaga gcaggaaaat atgaccctga ctgaactgaa
    39541 aaaggtcatt gccgacatct atcccggtca gacacagttt tatgtgattg aatttaaatg
    39601 tctttaaatt gaactcacgc ctgttagttg aaatttctca tttaatttca tgatttttag
    39661 gagattagtc tcaaatcaga atttatttta gctaacaggt gttcactgga actattctca
    39721 gttacgctgg agaggtatac atgtgcgtgt acgtttctcc ggagtaagtt atggttcaga
    39781 agcccctcat taagcaggga tattcgctgg cagaggaaat tgccaacagc gtcagtcacg
    39841 gcattgggtt ggtgtttggt atcgttgggc tggtgttgct actggttcag gcggtggatc
    39901 tcaatgccag cgccacagcg ataaccagct acagcctcta tggcggcagt atgatcctgc
    39961 tgttcctcgc ttcgacgctc tatcacgcca ttcctcatca acgggcaaaa atgtggctga
    40021 agaaatttga ccattgcgct atttacctgt tgattgccgg aacctacacg ccgtttttgc
    40081 tggtggggct ggattctccg ttagcgcgcg ggttgatgat tgttatctgg agcctggcat
    40141 tgctggggat tctgtttaaa ctgaccatcg cgcaccgatt caaaatttta tctctggtga
    40201 cctatctggc gatgggctgg ctgtcgctgg tggtaattta tgaaatggca gttaagctcg
    40261 cggcgggcag cgttacctta ttggcggtag gcggcgtggt ttattcgctc ggagtgattt
    40321 tctatgtctg caaacgcatt ccctacaatc atgccatctg gcacggcttc gtgctcggcg
    40381 gcagtgtgtg ccactttctg gcgatctatt tgtatattgg gcaggcgtaa tgggtatgc
//
LOCUS       DAZGSP010000043        39098 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZGSP010000043 DAZGSP010000000
VERSION     DAZGSP010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.043"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(136..327)
                     /gene="yfgG"
                     /locus_tag="ACHK74_002368"
     CDS             complement(136..327)
                     /gene="yfgG"
                     /locus_tag="ACHK74_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFP0221179.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            345..497
                     /locus_tag="ACHK74_002369"
     CDS             345..497
                     /locus_tag="ACHK74_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221180.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            679..2922
                     /gene="pdeF"
                     /locus_tag="ACHK74_002370"
     CDS             679..2922
                     /gene="pdeF"
                     /locus_tag="ACHK74_002370"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFP0221181.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2961..4502)
                     /gene="ppx"
                     /locus_tag="ACHK74_002371"
     CDS             complement(2961..4502)
                     /gene="ppx"
                     /locus_tag="ACHK74_002371"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFP0221182.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4507..6573)
                     /gene="ppk1"
                     /locus_tag="ACHK74_002372"
     CDS             complement(4507..6573)
                     /gene="ppk1"
                     /locus_tag="ACHK74_002372"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFP0221183.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6744..7382)
                     /gene="purN"
                     /locus_tag="ACHK74_002373"
     CDS             complement(6744..7382)
                     /gene="purN"
                     /locus_tag="ACHK74_002373"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFP0221184.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7382..8419)
                     /gene="purM"
                     /locus_tag="ACHK74_002374"
     CDS             complement(7382..8419)
                     /gene="purM"
                     /locus_tag="ACHK74_002374"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFP0221185.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALAMLNSNGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8744..9370
                     /gene="upp"
                     /locus_tag="ACHK74_002375"
     CDS             8744..9370
                     /gene="upp"
                     /locus_tag="ACHK74_002375"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFP0221186.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9456..10745
                     /gene="uraA"
                     /locus_tag="ACHK74_002376"
     CDS             9456..10745
                     /gene="uraA"
                     /locus_tag="ACHK74_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFP0221187.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10840..11541
                     /gene="hda"
                     /locus_tag="ACHK74_002377"
     CDS             10840..11541
                     /gene="hda"
                     /locus_tag="ACHK74_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFP0221188.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11679..12038)
                     /gene="arsC"
                     /locus_tag="ACHK74_002378"
     CDS             complement(11679..12038)
                     /gene="arsC"
                     /locus_tag="ACHK74_002378"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFP0221189.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(12059..13522)
                     /gene="bepA"
                     /locus_tag="ACHK74_002379"
     CDS             complement(12059..13522)
                     /gene="bepA"
                     /locus_tag="ACHK74_002379"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFP0221190.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13735..14796
                     /gene="yfgO"
                     /locus_tag="ACHK74_002380"
     CDS             13735..14796
                     /gene="yfgO"
                     /locus_tag="ACHK74_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP0221191.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14834..15682)
                     /gene="focB"
                     /locus_tag="ACHK74_002381"
     CDS             complement(14834..15682)
                     /gene="focB"
                     /locus_tag="ACHK74_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFP0221192.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(15704..17716)
                     /gene="hyfR"
                     /locus_tag="ACHK74_002382"
     CDS             complement(15704..17716)
                     /gene="hyfR"
                     /locus_tag="ACHK74_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFP0221193.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTAKTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESVLLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17746..18159)
                     /gene="hyfJ"
                     /locus_tag="ACHK74_002383"
     CDS             complement(17746..18159)
                     /gene="hyfJ"
                     /locus_tag="ACHK74_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFP0221194.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(18152..18910)
                     /gene="hyfI"
                     /locus_tag="ACHK74_002384"
     CDS             complement(18152..18910)
                     /gene="hyfI"
                     /locus_tag="ACHK74_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFP0221195.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAKRFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(18907..19452)
                     /gene="hyfH"
                     /locus_tag="ACHK74_002385"
     CDS             complement(18907..19452)
                     /gene="hyfH"
                     /locus_tag="ACHK74_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFP0221196.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(19462..21129)
                     /gene="hyfG"
                     /locus_tag="ACHK74_002386"
     CDS             complement(19462..21129)
                     /gene="hyfG"
                     /locus_tag="ACHK74_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFP0221197.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(21119..22699)
                     /gene="hyfF"
                     /locus_tag="ACHK74_002387"
     CDS             complement(21119..22699)
                     /gene="hyfF"
                     /locus_tag="ACHK74_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFP0221198.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22704..23354)
                     /gene="hyfE"
                     /locus_tag="ACHK74_002388"
     CDS             complement(22704..23354)
                     /gene="hyfE"
                     /locus_tag="ACHK74_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFP0221199.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(23366..24803)
                     /gene="hyfD"
                     /locus_tag="ACHK74_002389"
                     /pseudo
     CDS             complement(23366..24803)
                     /gene="hyfD"
                     /locus_tag="ACHK74_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(24820..25767)
                     /gene="hyfC"
                     /locus_tag="ACHK74_002390"
     CDS             complement(24820..25767)
                     /gene="hyfC"
                     /locus_tag="ACHK74_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFP0221200.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTCISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMASLFVALFLSFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFCLAALAWVFWLTGL"
     gene            complement(25778..27796)
                     /gene="hyfB"
                     /locus_tag="ACHK74_002391"
     CDS             complement(25778..27796)
                     /gene="hyfB"
                     /locus_tag="ACHK74_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFP0221201.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNTFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQNIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIAFVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27796..28326)
                     /locus_tag="ACHK74_002392"
     CDS             complement(27796..28326)
                     /locus_tag="ACHK74_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP0221202.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPFGAISASGSRPVNAPAQYVFQAEGSLKDGEENAPPQHP
                     LLRWEPGVQTVAVKCDLCDFLPEGPACVRACPNQALRLITDDSLQRQMKEKQRLAASW
                     FANGGEDPLSLTQEQR"
     gene            complement(28579..29049)
                     /gene="bcp"
                     /locus_tag="ACHK74_002393"
     CDS             complement(28579..29049)
                     /gene="bcp"
                     /locus_tag="ACHK74_002393"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFP0221203.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(29049..29621)
                     /gene="gcvR"
                     /locus_tag="ACHK74_002394"
     CDS             complement(29049..29621)
                     /gene="gcvR"
                     /locus_tag="ACHK74_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFP0221204.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDTHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29767..30645
                     /gene="dapA"
                     /locus_tag="ACHK74_002395"
     CDS             29767..30645
                     /gene="dapA"
                     /locus_tag="ACHK74_002395"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFP0221205.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30662..31696
                     /gene="bamC"
                     /locus_tag="ACHK74_002396"
     CDS             30662..31696
                     /gene="bamC"
                     /locus_tag="ACHK74_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFP0221206.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31909..32622
                     /gene="purC"
                     /locus_tag="ACHK74_002397"
     CDS             31909..32622
                     /gene="purC"
                     /locus_tag="ACHK74_002397"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFP0221207.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32776..33003
                     /gene="vapB"
                     /locus_tag="ACHK74_002398"
     CDS             32776..33003
                     /gene="vapB"
                     /locus_tag="ACHK74_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFP0221208.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            33003..33401
                     /locus_tag="ACHK74_002399"
     CDS             33003..33401
                     /locus_tag="ACHK74_002399"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFP0221209.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            33548..34411
                     /gene="ypfJ"
                     /locus_tag="ACHK74_002400"
     CDS             33548..34411
                     /gene="ypfJ"
                     /locus_tag="ACHK74_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFP0221210.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            34426..36441
                     /gene="tmcA"
                     /locus_tag="ACHK74_002401"
     CDS             34426..36441
                     /gene="tmcA"
                     /locus_tag="ACHK74_002401"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFP0221211.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            36515..37213
                     /gene="ypfH"
                     /locus_tag="ACHK74_002402"
     CDS             36515..37213
                     /gene="ypfH"
                     /locus_tag="ACHK74_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP0221212.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(37294..37494)
                     /gene="ypfN"
                     /locus_tag="ACHK74_002403"
     CDS             complement(37294..37494)
                     /gene="ypfN"
                     /locus_tag="ACHK74_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFP0221213.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(37522..38649)
                     /gene="dapE"
                     /locus_tag="ACHK74_002404"
     CDS             complement(37522..38649)
                     /gene="dapE"
                     /locus_tag="ACHK74_002404"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFP0221214.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38653..39009)
                     /gene="yffB"
                     /locus_tag="ACHK74_002405"
     CDS             complement(38653..39009)
                     /gene="yffB"
                     /locus_tag="ACHK74_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFP0221215.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9571 a        10019 c        10273 g         9235 t
ORIGIN      
        1 acaggataaa catcagtcag gacaaagacg taaaatttaa gatgaaagaa tatctgccgc
       61 tttgataatt ccaaagagca aaagcatccg acagcgaagt gccgttaggt tctgtgaaag
      121 tggagaaggt aaccgctaac gttgtaccgg tgattcgacg gagagtgtgg attgctgagc
      181 ttcttttttg ctctgatggt gctgccaggc accgacggac gagtagataa aacggccaaa
      241 gaagaagata aagctgatga gcagtacgat acgggtcatg cgactgttaa atcggtgtcg
      301 ttttcgcata ctggttgcct gactcacaaa aggttccttg aagtatgtcc cacgctgtgg
      361 acggtactta cattaaggca caacaggaca aaatggtcaa ttcttcgtta tgtaaaaaag
      421 cgtcagtgga tacatatttt aatgttatgg aagttaattt aattatttac aatgatgatg
      481 taataatgat gaattaacat gataaacgta ataattcatt aatcagggtt attttgtttg
      541 atatatatca attggaactt tcatatgaca cttagaatca atgctctcta tagtgatgaa
      601 taatcatcat tcgaagtcag gtgggatgcc tgtctgaata cacctccttc aggatgtggg
      661 ggattctgct gagcatctat gaaactgaat gcaacttata taaaaatacg tgataaatgg
      721 tgggggcttc cgctgttcct gccttcttta atcttgccca ttttcgccca cattaatact
      781 ttcgcgcata tttcttccgg tgaggttttt ctcttttatc tgcctttggc actgatgatc
      841 agcatgatga tgtttttcag ctgggcggca ttgccaggga tcaccttagg gatttttgtc
      901 cgcaaatatg cagagctggg tttttacgaa acgctctcat taacggctaa ttttattatc
      961 attatcattc tctgttgggg cggttacagg gtctttactc cccggcgtaa caacgtttca
     1021 catggtgata cccgtttaat ttcccagcgt atattctggc agatagtgtt tcctgcaacg
     1081 ctgtttctga tacttttcca gtttgctgca tttgtaggat tgctggcgag cagagaaaat
     1141 ctggtcggtg tcatgccttt taacctcggg actttaatca attatcaggc cttactggtg
     1201 ggtaatctga tcggtgtccc gctgtgctac ttcatcattc gggtagtgcg aaatccattt
     1261 tatttacgta gctattattc gcaattaaaa cagcaggttg atgccaaagt caccaaaaaa
     1321 gagttcgcgc tctggctact ggcattaggt gctttattgt tgctgttatg catgccgtta
     1381 aatgaaaaaa gcacaatttt tagcaccaat tataccttgt cattattgct gcccctgatg
     1441 atgtggggag cgatgcgcta tggttataag ctgatttcgc tgctctgggc ggtcgtgttg
     1501 atgatcagca tccacagcta tcaaaattac attcccattt atcctggcta taccacgcag
     1561 ctgaccataa cctcctccag ttatctggta ttctctttta ttgtcaatta tatggctgta
     1621 ctggcaactc gtcagcgagc ggtagtcaga cgcattcagc ggcttgcgta tgtggacccg
     1681 gtggttcatc tgccaaatgt tcgcgccctg aatcgcgcgt tgcgtgatgc cccctggtct
     1741 gcgctttgtt atttacgcat ccctggcatg gaaatgctgg ttaagaacta tggcatcatg
     1801 ctgcggattc aatacaagca aaaactttct cactggctgt cacccttgct ggaaccgggt
     1861 gaagatgttt atcagctttc ggggaacgat ctcgcgctgc ggctgaatac agaatcgcac
     1921 caggagcgca ttaccgcact ggatagccat ctcaagcaat ttcgtttctt ttgggatggc
     1981 atgccgatgc aaccgcagat tggcgtcagt tactgctatg tgcgctcgcc agtgaatcat
     2041 atctacctgc tgctgggaga gctaaatacg gtcgccgaac tttccatcgt gaccaacgcc
     2101 ccggaaaata tgcagcgtcg cggggcaatg tatttgcaac gcgaattgaa agataaagtc
     2161 gcgatgatga atcgactaca gcaggcgctg gaacacaacc attttttcct gatggcccag
     2221 ccgattaccg gtatgcgtgg tgatgtttac catgaaattc ttctgcgcat gaaaggtgag
     2281 aatgatgaac tgatcggccc cgatagcttc ttgccggtcg cgcacgaatt tggtttatcg
     2341 tcgagtatcg acatgtgggt cattgagcat acgctgcaat ttatggctga aaacagagcg
     2401 aagatgcccg ctcaccgttt tgctattaat ctgtctccaa cctcggtatg tcaggctcgt
     2461 tttcctgttg aagtcagtca gttgctggat aaatatcaga ttgaagcgtg gcaacttatt
     2521 tttgaagtca ccgaaagtaa tgctctgacc aatgttaagc aggcgcaaat caccttgcag
     2581 catcttcagg aattaggctg ccagattgcg attgatgatt tcggcaccgg ctacgccagc
     2641 tatgcgcggc ttaaaaatgt gaatgccgat ctgcttaaaa ttgacggcag ttttatccgc
     2701 aatattgtgt caaatagtct ggattatcag atagtggcat cgatttgcca cctggcgcga
     2761 atgaagaaaa tgcgggtagt ggcagagtac gttgaaaacg aagagatccg cgaggcggtg
     2821 ctctctttgg ggatcgatta tatgcagggt tatcttattg gtaagccgca accgttaatt
     2881 gatacgctga atgaaatcga acccattcgc gaaagtgcct gaataatgcg ggccgacatt
     2941 tctcgtcggc ccgcaaagca ttaagcggcg atttcaggtg tactttcttc ttcaattttc
     3001 aaccgccagc cagccacgcc ttcccagtat tcttgctcct tttccagatc aagcagtacc
     3061 agcgcattct gactaaacca gtcatgcggg aaacgcagtg tccagtgact gtcatcagta
     3121 atcagtgtca atgttggcgg tgtggtggtt gcctgacgtt gattgtttag taatacgcca
     3181 aggcgcaata gctgtatcag tggcaggaac tgtttcttct taaacaaggt aaagcgcggc
     3241 agatcgtcga gcttaatcgc tttacggtga tagcgcacca gtgtcgccat catcagctgc
     3301 tgttcctgat taaaacccgg caagtcactg ttttgcagaa tataagcgga gtggcgatgc
     3361 aaaccgctgt ggttgatatt caacccgacc tcatgcagca tggcggccca tcgcagtagc
     3421 gcctccagtt gcggatgcgc cagcttcggt tgctgttccc gccactgttc gtacatttgc
     3481 atagtggtat ccagcacccg tcgggcctgc tcgctgtcga tgtgatactg gttggcgagg
     3541 ctgctggcgg tgcgactacg cacatcctga tgacggaaac gtccttccat ttcatacagt
     3601 acgccttcgc gtaacgcccc gtcagaaagg cgcagttcac ggatggctaa agcatcaaac
     3661 acaccgcata aaatcgccag tccaggaacg aagactgttt tccgctcttc ggaaagaccc
     3721 ggtaaactca gcgatgcgaa attacggtga cgtaaaactt cttttaccag tttttccaga
     3781 cgttccgggg taattatccc gtctttctcg cccatttcca ttaacacttc atgggcggct
     3841 tttatggtac cggaagcgcc cattgccacg ttccagccct gaatacggaa ttgccaggtt
     3901 aaagtttcca gtttttgtgc tgccgccata cgagcgcgct gaaaattctc tttattgatg
     3961 accccgccag ggaagtaaag ctgggcaaag ctgacacaac ccatccggcg gctttcaacg
     4021 agaatcggtt cgaaattttc accaatcacc agttccgtag atccgccgcc aatatcaata
     4081 accagtttgc gacctttctc cggttgggta tgttccacgc ccataaaaat cagacgggct
     4141 tcttcattac cggaaataat ttcaatcggg tagggaatga ccttttccgc gcgtttcaga
     4201 aagtcagtgg cgttcagcgc ctgacgcagc gtatgggtgc cgactataca gacgctggca
     4261 ggagaaaacc cttgtagccg ttcggcaaac agcgacagac agttcaaacc gcgcgtcatt
     4321 gcctcttcac tcaacatatt atctggcccc aggccgtccg ccagatgcac ccgctgtttc
     4381 aggcggccaa taatctgcat ggcaccatct accacacggg ctatgaccat gtgaaaactg
     4441 tttgaaccaa gatcgaccgc agcaaactcc tgcggacgag gggatttatc gtgtattggc
     4501 atagggttat tcaggttgtt cgagtgattt gatgtagtca taaatcgcca actgcgcccg
     4561 tactttgcgg cgattgccgc ggggaacgta gcgattactg agttctttat cgatataacg
     4621 agctttgacc gtatcgctga acaatatgtc gatgatgtcc agcacccgct gcttcaggcg
     4681 cggatcgagc agcggtgtcg ccacttcaat acgataatca atattgcgcg tcatccagtc
     4741 ggcggaagaa aggtagacct ttttatcgcc gccattttca aaaatataaa cccggtcatg
     4801 ttcaaggtaa cggtcaacaa tactgatggc tcgaatgttg tcgctaatgc cttccagatt
     4861 ggggatcagc gaacacattc cgcgaaccag cagattaacc ggtacgccgg agctggaggc
     4921 cgcatacaga cgatcaacca ggcctttatc gacaaggtta tttagcttca gggtgatacc
     4981 actgggcagc ccttgctgcg cgttggcgat ttcgcggtct accatttcat ataacagacg
     5041 gcgagagttt tgcggtgaca ccattaaata atcaaatgtc accgggcggt atgggttttc
     5101 aataaagtta aatacccgcc gtacttcgtt ggtgatacgc gcatctgcgg tcagcaacga
     5161 atagtcagta taaagacgcg cggttttttc gttaaagttc ccggtcccga tgtgtgcgta
     5221 acggaccact tcaccgtttt ctttacgtga aatcaggaac agtttggcgt gaattttcag
     5281 ccccggcgca gagaagataa cgtgcacgcc tgcttcggtc aggcgcttcg cccagtgaat
     5341 gttggcttct tcgtcgaaac gcgcctgtaa ctcaaccacc acagtgactt tcttaccgtt
     5401 atgtgcggcg tggatcatcg agtcgatgat gcgtgaatct ttcgccacgc ggtaaatgtt
     5461 aattttaatc gccagtacgc tcgggtcgaa cgaagcctga cgcagcagtt ccagcacatg
     5521 ctcaaaggtg tgataaggat aatagagcaa cacatcgcgt tcgcgaatgg catcaaaacc
     5581 attgcggaac tgggctttat caaaccaaat gtggcgtaaa cgcggcagtg gtttgttcac
     5641 cagattggct ttgccgacat tggggaaatt aataaagtct ttaaaattat gataacgacc
     5701 gccggggacg atggagtcgt agcgggaaat agtcagtttt tcgcgtaaca cttcaaccag
     5761 cgcattgggc atatcgcgct gataaacaaa acgcaccggc tcagcagtta aacgctgctt
     5821 gagactggaa gacatcaact ccatcaggct ggcttccatc tcatgcacta aatcgtattc
     5881 ggcatcgcgg gtcatcttca ttgaataggc attcagcgcg tcataatcaa agaagccttt
     5941 gaaaatatca tcaaggcagt aacgcagaat gttatccaga agaatcatcg gcttgcgtcg
     6001 acgcggtgct tctggcggta aattcacaaa gcgcggcact ttatctgatg ggatctccag
     6061 cagcgcgtaa cggatggtat cgccacggat aatttccacc gccagatagg tgtaatcatc
     6121 tttcaggaac tgcactaagt cagtgtcagg attgattaaa atcggcgtaa tgtgctgacg
     6181 cagatactgc ttaaaataat gacgaagcca gttttgttga ttgacggaga gctggcgttc
     6241 attaatcagg aagatctggt tacgcgccat ctccagcagc agctcgttgt agaggccgtc
     6301 gaattcctga tcggctttca gcacccggga ctgaattttg cccagtaaat ggcgggaatg
     6361 agagttggag ccttgttctt cgctaataat gatgcgtcgc ttcagttcag cgaagcggac
     6421 tttatagaac tcatcaaggt tattggaata gatccccagg aaacgcatcc tttcaatcag
     6481 cgggttagat ttgtccgccg cttcctgaag cacgcgttca ttgaacgata accaactgag
     6541 ctctttttcg atgtatagct tttcctgacc cattaccact tttactccgt tttattcacg
     6601 ggacactgcc tggatattat ggcgagcaat attggcagat gtccaactat gccagaaaag
     6661 ggggaaaaaa actgctggag cttgcgattt acaggtgaat tgaaaaacgc cagcggcaga
     6721 gctggcgctt taactacggg gcattactcg tcggcagcgt agccctgcgg cggcagacgt
     6781 tgaccatcca gccacgcggc gttttcgtgc attttcagac gaccatcggc aaaccagcta
     6841 atcaccagtg gataaatagc gtgttcctgg gtttgcacgc gggcggtgat gtcatcttcc
     6901 gtatcaccag caaataccgg gactttcgcc tgtaaaataa ccgggccacc gtccagttca
     6961 tcggtgacga aatgcaccga tgtaccgtgc tcttcatcac cattttccag cgcctgacga
     7021 tgggtgtgta atccgggata tttcggcagc agagaagggt gaatgttcag caaacgcccg
     7081 gcatagtggg agacaaacgc cgggctgaga atgcgcataa aaccagccag cacgaccaca
     7141 tcgggtgcgt acatgtcgat ttcatgaatc aactcccggt cataggcttc acgactgtca
     7201 aacgcgctgg cgatgagcgt atgcgttgca atacccgcct ggcgggcgcg ttcaaggccg
     7261 aacgcgtcgg ccttattgct gaaaactgcc cgtacggtgc ctttaatttt gttagtttta
     7321 caggcgtcaa taattgcctg taaattactt ccgttgccgg aaataagcac cacaatattc
     7381 attattcgat aaccacgcgt tgttcggaat cagaggcttt gatgataccg attttccacg
     7441 cgttttcacc gttggagttt agcatggcga gggctttgtc cacttccgga gcaggcaggg
     7501 cgataatcat cccgacgccg cagttgaagg tgcgatacat ttcatggcgc tcaacgttac
     7561 ctgccgtttg cagccagttg aacacttccg gccactgcca ggaagattca tcaatcactg
     7621 cctgagtatt atctggcaat acgcgtggaa tgttttccca gaagccgccg ccggtcaggt
     7681 gcgcaatggc atgcacatcg accttttcaa tcaactccag cactgacttc acgtaaatgc
     7741 gggtcggtgc cagcagatga tcggctaatg gcttaccatc aagttcggtg gtttgcggat
     7801 cacaaccgct gacttcaaga attttgcgca ccagcgaata gccgttcgag tgcggaccgc
     7861 tggaaccgag tgcaatcagc acatcgccgt cgctgacttt agagccgtcg atgatttctg
     7921 atttttctac cacgccaacg cagaaacccg cgacatcgta atcctcaccg tgatacatcc
     7981 ccggcatttc tgccgtttcg ccacccacca gtgaacagcc cgattgcaga catccttccg
     8041 cgataccgct gatcaccgct gaagcggtat caacatccag ttttccggtt gcgtaatagt
     8101 cgaggaaaaa cagcggctct gcaccttgca ccaccaggtc attaacgcac atggcgacca
     8161 gatcaatacc aatggtgtcg tgacgtttta agtccatcgc cagacgcagc ttggtaccta
     8221 cgccgtcagt accggaaacc agcacaggtt cacgatattt ttgcggcaat gcacacagcg
     8281 caccgaagcc gcccagaccg cccatcactt ccggacgacg cgttttcttc actacgcctt
     8341 tgattcttcc aaccagagca ttacccgcgt caatatcaac accggcatct ttgtagctaa
     8401 gagaggtttt atcggtcact gcttgggtcc ccacgcgtta cttgcggtag aaaaataaaa
     8461 ttcggcgcaa ttctaacagg gaaagcaaac gtttgcgaga ctgctttaca caaccttttt
     8521 gcacgtcttt tgcccaggca cgcggcgaaa gaagacttgt gccagggtaa aggttagttt
     8581 tcggatggaa taatcttctt tcataaccat ttgaatataa aataacttta tctcaaaccg
     8641 ttatcatttt gactaaagtc aacgaaaaga atattgccgc cttgaagaaa ggaggtataa
     8701 tccgtcgatt ttttttgtgg ctgcccctca aaggagaaag agtatgaaga tcgtggaagt
     8761 caaacaccca ctcgtcaaac acaagctggg actgatgcgt gagcaagata tcagcaccaa
     8821 gcgctttcgc gaactcgctt ccgaagtggg tagcctgctg acttacgaag cgaccgccga
     8881 cctcgaaacg gaaaaagtaa ctatcgaagg ctggaacggc ccggtagaaa tcgaccagat
     8941 caaaggtaag aaaattaccg ttgtgccaat tctgcgtgcg ggtcttggta tgatggacgg
     9001 tgtgctggaa aacgttccga gcgcgcgcat cagcgttgtc ggtatgtacc gtaatgaaga
     9061 aacgctggag ccggtaccgt acttccagaa actggtttct aacatcgatg agcgtatggc
     9121 gctgatcgtt gacccaatgc tggcaaccgg tggttccgtt atcgcgacca tcgacctgct
     9181 gaaaaaagcg ggctgcagca gcatcaaagt tctggtgctg gtcgctgcgc cagaaggtat
     9241 cgctgcgctg gaaaaagcgc acccggacgt cgaactgtat accgcatcga ttgaccaggg
     9301 actgaacgag cacggataca ttattccggg cctcggcgat gccggtgaca aaatctttgg
     9361 tacgaaataa agaataaaaa taattaaagc cgactttaag agtcggcttt tttttgagta
     9421 aagcgcctat aacacataat acagaggata atactatgac gcgccgtgct atcggggtga
     9481 gtgaaagacc gccactttta cagacaatcc cgcttagttt gcaacatttg ttcgccatgt
     9541 ttggtgcaac cgtcctggtg cccgtcttat ttcatattaa cccggcgact gtactgttat
     9601 ttaacggtat tggaacgctg ctgtatctct tcatctgtaa agggaaaatt ccggcttatc
     9661 ttggttccag ctttgccttt atttcaccgg tattgttact gttgccgttg gggtatgaag
     9721 tcgcgctggg cggctttatt atgtgcggcg tgctgttctg cctggtttct tttatcgtga
     9781 agaaagcggg gaccggctgg ctggacgtgc tgtttccacc tgcggcaatg ggcgcaatcg
     9841 ttgccgtcat cggtctggag ctggcgggcg tagctgccgg tatggcgggt ttactcccgg
     9901 ctgaagggca aacgccagac tccaaaacca tcatcatctc tattaccacc ctggcggtca
     9961 cggttttagg ttccgtgctg ttccgtggtt tcctggcaat tatcccgatt ttaattggcg
    10021 tgctggtggg gtacgcgctc tctttcgcaa tgggaattgt cgataccacg ccgattatta
    10081 atgctcactg gtttgcgctg ccaaccctct atacgccgcg cttcgagtgg tttgccattc
    10141 tgactattct gccagcggca ttagtggtta ttgccgaaca cgtagggcac ctggtagtaa
    10201 cggctaatat cgtcaaaaaa gatctgctgc gcgatccagg tctgcaccgt tcgatgtttg
    10261 ctaatggctt gtcgaccgtg atctccggct tctttggctc tacgccaaat accacttacg
    10321 gagaaaacat tggcgtgatg gcgatcaccc gtgtttacag tacctgggtt atcggcgggg
    10381 cggcgatttt cgctatcctg ctttcctgcg tcggtaaact ggctgccgct atccagatga
    10441 tcccattgcc ggtgatgggc ggcgtttcgc tgctgcttta tggtgtcatc ggtgcttccg
    10501 gtattcgtgt tttgatcgaa tcgaaagtgg attacaacaa agcacagaac ctgatcctga
    10561 cttccgtgat tttgatcatc ggcgtcagtg gcgcgaaggt aaacatcggc gcggcggagt
    10621 taaaaggtat ggcgctggcg accatcgtcg gtatcggctt aagtttgatt ttcaaactga
    10681 tcagtgtgct gcgccctgaa gaagtggtgc tggacgcaga agacgcggat ataacagaca
    10741 aataatgtac cgaccgggca gtgttgctgc ccggttcaaa catcatggga ttctgtggta
    10801 aacttctcgc gattttgtga aatcctggtt gaggtatctc tgaacacacc ggcacagctc
    10861 tctttgccac tttatcttcc tgacgacgaa acctttgcaa gtttctggcc gggggataac
    10921 tcctctttac tggccgcgct gcaaaacgtg ctgcgtcagg aacatagcgg ttacatctat
    10981 ctctgggcac gcgaaggcgc ggggcgcagc catctgctgc acgcggcttg cgcggaattg
    11041 tcgcagcgtg gcgatgcggt gggctatgtc ccgctggata aacgcacctg gtttgttccg
    11101 gaagtgctcg acggtatgga gcatttgtcg ctggtctgta tcgacaacat tgagtgtatt
    11161 gcaggcgatg agttgtggga gatggcgatt ttcgatctct acaatcgaat tctggaatcg
    11221 ggcaaaacac ggttgttgat caccggcgat cgtccaccgc ggcagttgaa tctgggatta
    11281 ccggatctcg cgtcgcgact cgactggggg cagatctaca aattgcagcc actttctgat
    11341 gaagataagt tgcaggcgct acagttacgc gcgcgtttgc gtggttttga actgccggaa
    11401 gatgtggggc gtttcttgct gaagcggctc gacagagaaa tgcgcacgct atttatgacg
    11461 ttggatcagt tggatcgtgc gtcgattacc gcgcaacgta agttgaccat tccgtttgtg
    11521 aaagaaattc tgaagttgta gataaagtgt ttattgtcgg atgcggcgcg aacgccttat
    11581 ccgaactacg cgaagttaat ccatagtatt cgtaggccgg ataagacgtg gcaagcgtcg
    11641 catccggcaa tgtgcttcaa ctagcgatgg agcacatctt aacccacgat ttccagtacc
    11701 tgctccggtg gacgaccaat ccgcgctttg ccattcgcga ccacaatcgg gcgctccatc
    11761 agcttcggat tatccaccat tgcctgaatc agcgcctctt cgctaagcga actgtcagcc
    11821 aggttcagtt ctttataaag atcctctttc tggcgcatca attcacgggc gctattcatc
    11881 cccagcattt tcagcaaatc gcgcagcgtt gccgcatcgg cgggtgtctc aaggtagagc
    11941 accacttctg gctccacgcc gttttctttc agcaggttca gcgtttcccg gctctttgaa
    12001 cagcgtgggt tatggtagat ttttacctgt ttggtcattg ttcttccttt aatgcgaatt
    12061 acatcttggt ataaggctta aagcgttcct gcagctggcg caactggtcg atgcgcgcat
    12121 cgtaacgcgc ttgttgcagg ctgcctaatt tcacctgcga actggcgcta ctcaacagcg
    12181 aaatggcctg atcgagtcgt ccggcgagcg cataaccttc tgctcgcgca gccagctcct
    12241 gatcgcggtt atttagcgcg gcttccgcct gtgccagcaa atcccagccg ttgctgtcat
    12301 ctttattatt aaaggtgtag cgattgagaa tattggccgc ttcttgtggt tgaccgcctt
    12361 gcagataagc gttcgccagg ttgagctgca acaccggatt ggtgcgtaaa tcgcgggcat
    12421 ttttcagacg attgatcgcc tcattggctt tgttttgccc aagatcgata tcagtagcca
    12481 gatcgagata ccatgcgttg ccaggttctg ccgccagtaa cggttgcagc gttttacgcg
    12541 cctcgtcgta tttattggct tccatcgcct gtaaagcacg accatattgc gccgctcgtt
    12601 gctgacgaac gtttcctttc gcccattcat ccagcaaatc actggtgagc tggttacgtc
    12661 cggaattata catccccagt gtgcgcgctt tcgccagata gaaatcttcc gacgactgca
    12721 ccaccatcgg tcgcatctga ttagcacggt tgcgggcatc tgccagacga ctttccggca
    12781 acgggtgagt caataaaatt tccggcgggc gcgaggagta acgcgcctga tcgagtaatt
    12841 tttcgaggaa ggttggcatc gcctgcggat cgaatcccga gcgttgcagc acctgaatac
    12901 caatgcggtc cgcttcctgt tcattttgct gggtgaaact gatcatcccc tgacgtgttc
    12961 ccgccagtgt accggtcagc gccgccatcc ccgcctgcgg actggccatc gccagtaaaa
    13021 tagaacctaa cgcgccgacc caggtcagcg gcgcgctgcg ttgttgatct tccatcgctc
    13081 gcgccaggtg acgttgggtg acgtgggaga tttcgtgcgc cataactgaa gccagttgac
    13141 tttcgttatc ggaataacgg aacagggcag agtgcagcac cacgttgccg ccaaagaaag
    13201 caaaggcgtt aatttcgtcg ttgttgatca gaaaaaaatg aaacggtgtc ttaaccgaat
    13261 tggcatgcga aaccagacgc atccccagcg aattaatata ttgcgttaac agcgggtcat
    13321 taattaacgg cgcgctgccg cgtagctggc ggacataata gtcgcccatc tgcatttcct
    13381 gaccaatgga aagcgtgctt cctgcggagg ttcccatatc cggcaaggtg tctgcgctgt
    13441 cggcaaacgc cggggctacc tgaccaatgg tcatagcagc aatgagggtt gcaaccaggt
    13501 tttttttcaa ctgcctgaac ataacctcta tcctgtattt ctgaagatag tcatttgacc
    13561 gatactgtta aatatcgttc ccaccgcgcc gcctttgagt gtaacggcga agattagccc
    13621 actcaaatga cttttcagtt tcctgaatcc gtcataaaaa gcgaagtctt ttttgtgaca
    13681 tttcgataca attgcggatc gcaatcccgc tcttgaggtt cagggaaggt ctttatgctc
    13741 gaaatgttga tgcaatggta tcgccgccgt tttagcgacc cggaagcgat tgccttgctg
    13801 gttattttag ttgccggatt tggcattatc tttttcttta gtggcctgct tgctccgttg
    13861 ctggtggcta ttgtgctggc ctatttgctg gaatggccaa ccgtgcgcct gcaatctatt
    13921 ggctgctccc gccgctgggc gacgtcgatt gtattggtgg tttttgtcgg tatattgcta
    13981 ctgatggcgt tcgtggtact gcctatcgcc tggcaacagg gcatctactt aatccgcgat
    14041 atgccgggga tgctcaataa gctttctgac tttgccgcca cgttgccgcg ccgctatccg
    14101 gcgttaatgg acgcgggcat tattgatgca atggccgaaa atatgcgcag tcggatgctg
    14161 accatgggcg attcggtggt gaaaatttcc ctcgcctcgc tggtcggttt gctgaccatt
    14221 gccgtctatc tggtgctggt gccattgatg gtcttcttcc tgctgaaaga caaagagcag
    14281 atgctgaacg ccgttcgccg ggtgctgccg cgtaaccgtg ggctggcagg acaggtgtgg
    14341 aaggagatga atcaacaaat caccaactat atccgcggca aagtgctgga gatgatcgtg
    14401 gtagggatcg ccacctggct ggggttcttg ctcttcgggc tgaactattc gctgctgctg
    14461 gcggtgctgg tcggcttctc ggttcttatt ccgtacattg gcgcatttgt ggtgaccatt
    14521 ccggtggttg gcgtggcgct attccagttt ggcgcaggca cggaattctg gagctgcttc
    14581 gcggtgtatc tgattattca ggcgctggac ggcaacctgc tagtaccggt gttgttctcc
    14641 gaagcggtta acctgcatcc gctggtgatt attttatcgg tggtgatctt cggtggtttg
    14701 tggggattct ggggcgtatt cttcgccatt ccattggcga cgctgatcaa agccgtgatc
    14761 cacgcctggc ccgatgggca aatcgcgcaa gaataatctt cagcccgacg aactgtttcg
    14821 ccgggctgat ttttcaggat tcctgacgta aataaatagc ccgataacaa atactcacca
    14881 gcactgcacc gccgataata ttacccagca tcaccgggag cagattggcg gtaataaaat
    14941 ggctgaccgt cagtgccgga aaattgtctg cgctactgtg cgccagttgc cagaaggagg
    15001 taggggcgaa atggcgaatg gcaatggcga acggaatcac aaacaaattg gcgatacagt
    15061 gctcaaagcc actggcgaca aacagggtga tgggcaaaat catggcgacg attttgtcgc
    15121 ataacgaacg cccgcaataa ctcatccaca gcgccaggca aaccatcaga ttgcacataa
    15181 tgccgaggct gacagattca gtaaatgtat gatgcatttt gccctcggcg cagtgtaaaa
    15241 ccgcgactcc ccacatcgcg ttttcactca tcaccagccc ggaaaaccag attaacaaac
    15301 tgaaacaggc aatacctgcc agattaccgc aggccaccag aagtgcgtta atcagccaag
    15361 ttcgccaact aataacgccc cgactttttg ccattaccgt cattaccgac gaggtgaaca
    15421 ggttggtgcc gcaaaccgcc agcaaaataa agccaagtgt aaagcaaagg ccgcccacca
    15481 gatgggttaa tgcctgtgaa gacggggcat cggcgataac tgaaaggtaa aaagtaaatc
    15541 cgatcgccat aaacacgcca gcggacatcg ccattaaaaa gaccgacact ttactgcggg
    15601 caattttatg ggcagcaatc cgttcagcga ttgccgcttt tctggcgctc aactgcaagt
    15661 cgaaagagag tttgtttctc atgcatactc ctgcaaaagc agattacaac acctcgcgaa
    15721 ccgagattcc cagacgctgc attcgtgaca gcagcgtggt gcgcttcatc cctaatcgtg
    15781 tcgccgcgcc acggggcccg gcaacaatgc cattggtttc tcgcaatacc tgaacaatgc
    15841 gctgacgttc ttcttcgtca ttctctggcg tcgtcgggtg cagcaactgc gccattgaac
    15901 tgcgaagcgc tgaatcttca tttagcgtcg gcagtaaacg gctttgtcgg acattaagat
    15961 gtaaattcag actgttaccg cgagtcagca gtaccgctcg ctcaatcacg ttttccagct
    16021 cgcgcacgtt gcccggccag tcccacgaca tcaactggcg tagcgcctcg gtcgggatgg
    16081 cgtcaattga acgattcata tggcgcgcca ttttttgcgt gaaatgcttt gctaaaagag
    16141 ggatatcttc cggacggtcg cgcagcggcg gcaattccag tgggaagaca ttcaggcgat
    16201 aaaagagatc gctgcgaaac tggcgatctt caaccatttg ccacaaatca cggttggtgg
    16261 cggcaatgac tctgacattt accgggatcg ttctactccc gccgagacgc tcaatctccc
    16321 gttcctgcaa tacgcgcagc agtttaggct gaagttctaa cggcagatcg ccaatttcat
    16381 cgagaaacaa cgtgccgcca tcggcaattt caaaacgacc acgatgggta ttaatcgcac
    16441 cagtaaacgc ccctttgtca tgaccgaata actcactttc cagaagactg gcggggatgg
    16501 cagcgcagtt gattttgacc agcggcttgt cgcgtcgcgg gctaagttga tggatcgctc
    16561 tggcgatcac ctctttgccg gttccggttt caccgcaaat caacaccgta ctgtcgctct
    16621 tcgccacaat atctacctgc tggagcaggt cttccattgc ctggctttga tagataatgt
    16681 caccgatgcc cagattcgaa aggagctgct cgctaagctg gtggttttct tgctgcaaac
    16741 tttcctgcaa atcggtcatg ctacgccagg catcggcatt gccaacggca atagcgatgc
    16801 gatcggctat gtgttgtagt agctggcagt tttcctcact aaagagagtg gaagaggtat
    16861 gcgccagcaa caatgcaccc ggtgtatggt tgccaaaggt aagcggtata aggagcacag
    16921 attcgcagcc gttagatgca agtaagagta ataacgggtc gcgttgccag agaaacagat
    16981 cgtctgcacg gtgcgtcaag gtcggttgtc gggtttgtag cgtttgtgtc agcaatacac
    17041 tgtcgccagg catattgcgt ggcagacacg cacaatgtga ggcagaaaga tcgctgcacc
    17101 acaggctaaa cttctcgttc tttcgatgat cgcccagtac catactgact gaagccagac
    17161 cgaaaaaatg atggatctca cgagcgacgt cagcgatcag atcgtcgaga tcaagatgtg
    17221 acagcactgc attagtaata tcaaccagta cccgatgatg atcgcgatct ttctccagca
    17281 aacgcagcgc cacctgctgt tcttcatcat tgagccatga ttgcagaacc ggggtgatta
    17341 tccgcgccag ccagttaaac agtgtgcaat cctggggagt aaaacggctg ccgtgggtgc
    17401 gaataaagcg tagctccgcc attccactac tgccgagaag gacggcgtga cgcgaataat
    17461 cgaccatctc gccctgatac tctgtttttg ccgtcacatc gctgccatca tcatggtagc
    17521 ggtaatagaa gttgcttttg agaacatgca gttcaataag ttgtaccggg ctgaacggcg
    17581 gcggcatcgg gatgaaggcg cgtaataaag acgccttacc gtgtttctgt gctaaccgct
    17641 ccgcgagcat tccgtttacc gcttcaattg ttattccttg tggcggggca aacatcgcct
    17701 cgtctgacat agccatcaac agtccttatt aatgagaaat ccttctcacg caatttttct
    17761 ggccatcaga tagatggccg gttcctgcac aatttcatcc aacagatgaa tgaattgaat
    17821 gctacgcgcc tgccacacag acgtcgcgtc atccgccagt ggcgcgaatg cccgggtcaa
    17881 cagggcggtg tggctggcgt caataacaat ctcaccaaaa gtcatcaccc ccagcatctt
    17941 acgtcgggct tgctcctctt cgacctgggc aagccaatct tcgtattccg tcagtgggca
    18001 ggggaaggcg acattcagac aatcaatcac gccaacgtga tgaccgatag ccagcgagta
    18061 atacattacc tgagagctgt gttccggcat ctcgtcgcta ctggcgacaa acttttttcg
    18121 cagcgtccag aaaacaattt ccccgcactc ttcagtcatg taacccccgg agtacgcgaa
    18181 agagttgctg aacgatacta ttgagacgtg gatcgtcggc atcgcgcaac caggtgttaa
    18241 cccgatttcc tgtaggatcg tcgcttaaat ggcgcaggag ccgatcgcaa atttctcgcc
    18301 cctgacgata gcccgccagc cgccgcgctt ctcgctcaat ggcgatacgc tgtgatggcg
    18361 ggatctgtgg ccacaacggt tgcatagtca ccccggtggg atcgcgataa tccacagcgt
    18421 gaatcttctg ttgcagcaaa ccgagcgcca cggcgaaacc gtgaatggtg gcggccggtg
    18481 ttggcgggca gccggggatc caaacatcaa tggggacaat ggtgtcgctg ccgccccaga
    18541 cgctgtagag atcgtggaaa ataccgccgc cgacaccgca cgcgccgtag gaaacacaga
    18601 ttttatgatc gggggcagac tcatacgccc gaagtgcagg catacgcatc gcccgggtga
    18661 ctgcgcctgt aaataacaaa atatcggcgt gacgcggtga tgaaataacc ttaatgccaa
    18721 aacgttttgc gtcgaatact ggtgtgatgg cggcaaaaat ttcgatttca caggcgttgc
    18781 agccgccgca atcgacgcga taaacgtaag ccgagcgacg gatatcctgt agcaaatgct
    18841 gcttcatctt ttgcgtttgc tcgtccagcg tgatgggctg gctgacatgt tgtgtaagca
    18901 ctggactcat agctgctcct tagccaccag cggtacatct gtatcgtcgt tgagcagcgt
    18961 cgcgcgttgt ttgcactccg ggcagacgct cgcttgcgcc cataacattt cgcggttttg
    19021 tggcgcattt tgttgctgtg ccaacaattc agcagccagt gcgacggttt tttgcggggc
    19081 aaacgggcgt tcgcaacggc tgcaacgttg tagatggaac gtcgcgcggg tatagagatc
    19141 ggctttattg gtgacggtca gttcaaagtt attggtaagc tggatggctc tggtcgggca
    19201 cacttcttca caacgtccgc agtaaataca acgcctcaga tagagctgcc aggtgcgcga
    19261 attttgctgg tcgtcggtct ggatagtcag cgcatttgcc ggacaagcac aggcgcaggc
    19321 accgcaggca atacattggc tgggcatcag gtccggtttc ccgcgaaagc ctgggctgac
    19381 ctccagtggc gcgaagggat atttcaccgt cgcggttccg gcgcgcataa tagttttcag
    19441 taacttcagc atcttctgtc cttacttcag cggcgaacgg ttacgatcaa taccgtagcg
    19501 ttcgatctct ttatacggca cggtttttga ctggcgcttg cgcacatcta ccagcgtcac
    19561 acggtcggta caggagtagc agggatcaag gctaccgata atcagcggtg cgtcagaaac
    19621 ggtattgccg cgcagcatgt aacgcaacac cggccagttg gcgtaggtgg cggcacggca
    19681 gcgccagcgg aacaattttt ggttatcacc gagcatgctc cagtgcacgt cttcaccgcg
    19741 tggcgcttca acaaagccca gcgcgaatgc gtgaggttta tagctaaagc cttcggtcag
    19801 cagtggggta tccggcatgt tgtcgagggc aaattccagc attgccagcg aatcaaacgt
    19861 ctctttgaca cggaccatca cgcgggagaa aacatcgccg ccggtaaagg taaacagtgt
    19921 ttttggaata ttgccgtagt cggcgtaggg gtgatcaaag cgcaaatcac gggcgatttg
    19981 tcggtcgaga atgccaatgc cctgagtgcg ttgttccata ttcggcgtag caagcagcat
    20041 ctctaccagc tccgacacgt cggcgcgcat ctcgcgcacc agtttcagcg tttgcagacg
    20101 ttgctctttg agaatatcgc ggcgaacacc accaatcaga ttcagaccgt aggttttacg
    20161 cgacccgatc agcaattccg ccatcgtcat cgacttttcc cgcacgcgga aaaattgcat
    20221 aaagccggta tcaaaaccaa cgaaatggca ggagaggcca aggttaagca aatgactgtg
    20281 tagccgttcg acttccagca gaatcgagcg aatagtatgt gctcgttgcg gcacctcaat
    20341 ccccagtgca ttttcaaccg agttggtata ggccacactg tgggcaaaac cgcaaatccc
    20401 acacacgcgg tccgataaga aggtcacttc gttgtagccc atccgcgttt ctgccagttt
    20461 ctccatgccg cgatggacat aaaacaggcg gtaatcagca tcgacaattt gctcgccatc
    20521 cacaaacaag cggaagtgac ccggttcatc ggaggtgata tgcagcgggc cgacagggat
    20581 cacccgcgca tcgctgttgc cctcattgat aaacgcatac gtttcggaat ctgtcgtcgg
    20641 ttcagggcgc agtcgataat ccatcgcatc tttgcgcagc ggatgcatat cttccggcca
    20701 gtcatcgggc aacaccaggc gacgctgatc cggcaggcca accggaatca gcccgtacat
    20761 atcgcgaatt tctcgctcgc cccagaccgc ggcagggacg cgcggtgtga ctgacggaaa
    20821 ctcccgactg tcggcatcga ccagtgcctt caccacgatc cagcattttt cggccccttc
    20881 cattgaaagg gcataataaa ccgcgtaatg accgttaagt gtccgctcgt cgttgccaaa
    20941 caggactgga agccagccat catgttgata ataaagataa tgtacaacgt caggcagcag
    21001 attgattttc accgtaatgg tgacctgttc aggcgtttgt cgctcttcat ccagcaccgc
    21061 gccggggaac tgcgttttca gggcggcgag aatcgcttcg ccacgatttg acgatgaatt
    21121 aacgttcact gtgtttctcc gaaacagatg cggtgcctga aggcaaaaaa tcatgccagg
    21181 tgctacgttg tgcaggcagg tcgtgcgtcc ctgagaggac tatagtggaa gcgcccgcca
    21241 ggatcctgat gacaggttgt ggaatatgcg ttcccatcgc cagcatcatg accagcagaa
    21301 tcaccattgg cgaggtggtc aaccagccga gatcaccccg gttaacggcc tgcggcggtt
    21361 tcgccattaa cacccgcgct gccatccgta ccaggcccgc cagcaccagc gttaacagca
    21421 ataacagcag gacgataatc agcaggtgat tacgtgccag tccggcggta atggtcataa
    21481 attcgctaag aaaaatgttg aagggcggca tccctgccag cgccagcgca ccgccgccaa
    21541 acagcacggc ggtaaatggc atgattttga gcatcccaca gacgacgttg agatcgcgcg
    21601 tgccgtactt gagcagtaca ttgccggaac cgcagaacag cagcgttttt gccagactgt
    21661 ggtttaaggt gtgcagcagc gcggcaaaaa ttcccagtgg cccgccaatg cctagcgcca
    21721 ccgcgaccag tcccatgttc tccacgctgg agtacgccag cagacgctta atgtcccgct
    21781 gtaccagaat gaaaaatgcc gccacggcaa ccgacaacat gccgaagatg agcaacaacc
    21841 ggttggggaa atcgctgccg atggcctggc agataatgat gtaatagcga attagcacca
    21901 acagcgcgca gttcagcaat acggcggaga gcagggcgct gaccggactc ggtgcttcac
    21961 tgtgagcatc cggcagccag gcgtgcatgg gaaatagccc ggttttggtg ccaaagccaa
    22021 ttagcacaaa cacaaaggcc aacagcatta atgttgggtc aagcaaggac gattgcttaa
    22081 gaacctcgct ccagaatatc gccatttctg cctgcggcat aacgctggcg acgttggcgt
    22141 ataccagcac ggtaccgaac agaccaaaag cgacaccaac agtacaaata atgatgtact
    22201 tccatgctgc ttccagcgat gaacgctgac cgtaaatgcc taccagaaac gccgagctta
    22261 aggtggtggc ttcgatcgcc gcccacatca caatcaggtt attgctggta acaaccagca
    22321 gcatggtaaa caaaaacaga tggaagaaac cgtagtaatc gcacagcgta acgggggaaa
    22381 gctcgccgtg tgccacttca tgacgcatgt agccaatcga gtaaacaccg gtgagaaagc
    22441 caatcacacc aagaatggcg aggaacaaac cgcccagacc atcaatatga agccacagtc
    22501 ccgcagcgaa tatttctcct gcatcagcgg cagtttggac cacccagagt gccagaatca
    22561 gcagcagtgt gatccctaag ctatgtaata cagtcaccgt acaagtcgca gagagccccc
    22621 gtttccggca ggcaaaacag agcagcgaaa aaagcagcgg cgtgagcagg agtaaagcga
    22681 acatcacaga ataactcatc tcattatccc ttcagcgcgg tcaagttgtt cacgtccagc
    22741 gtgccgtggg tacgccatat ttttcttgcc agtaacacca tcacaatgac ggcgaagatg
    22801 gcgtcggtag cgatacctat ttccaccagt tccggtgctc gccaggcaag aagcgccagc
    22861 accagatggg agccgttttc catcaggcag taaccaaaaa tttgccgcag gatattgcgc
    22921 tggctgacaa tgcacagcag gccaagcaga aaatgaccta acgctaccgc cagcgccggt
    22981 ttcagcccgg tagccatcgg tagcttcacg ggctgaacga caaatgcgca aagcaggaca
    23041 attaacgccg cgagcagtgc catcattgcc ggaccgaata acgctttttc cgggatgttc
    23101 tggggaatat ttcgtgcagc gtaagtcatg attaacggta ccagcagcac tttggtgata
    23161 aaggcgctgg cggaccagat cagcagttgc tctgcggcga acaggcacga gagagtggcg
    23221 aaaatagaca ccagcaccag cgactggcag gcgtaaaatc cgcatgacag gcgatagctt
    23281 ttgacgctaa tcacaaacag cgatgtcagc atcatcagtc ccgccagatt atttacgatc
    23341 atagaaccgg tcattcatca tctccttact gcaaccaggt cgcggcgatt acgctggaaa
    23401 tcagcgacat cacaatcagt acaatcaaca ccaggcgcat tgaccctggc agcggtgcgg
    23461 catcggcgac ggcctcgctc ggtttgccag gcacaacgcg accaaaccag cgaataaacc
    23521 aggcgaaact ggcgaccgat tcaatcatca gcagaatcat ggcgggcagc aggatccagt
    23581 actccactga caacgcaaaa ccggcagcaa acagcgggaa tttactgaag aagccgttga
    23641 acggcggcac gccggtaatc gccagcgctg ccacgcagaa acccacgcct ggcaacggca
    23701 gggtgtgcaa tacgccacgc agacgcggca acaagcgcgt gccgcagctg taactcagcg
    23761 cacctgctac aaggaaaaac aggcttttag cgaacgcgtg gttgacgatg taggcgatgc
    23821 tgccctccag cgccagccgc gagccgaaga tggagagcga caaaccgaag aacatccagc
    23881 caagttgagt gatggtcgac caggccagca accgcttcat atcctgctgt ggcaaataca
    23941 tcagaaagcc ataaagaatg gtgaccagcg ccatgaccat gccaacgccg ccaatcacat
    24001 gcgggatatt gccgccgtcg ataatggcgc gggcaaaaat gtaaacgccc actttcacct
    24061 tcgatgcggc gtggagatag gcactgatcg gtgttggcgc ttccattgcg tccggtagcc
    24121 acgcttgcat cggtagctgg gccgatttcc cccacgcggc aaacaggatg ccgtcataaa
    24181 ccagataacg tgcgtcgcca tgtaactcgc tcatcgcgct aagcacaaac gttccggtct
    24241 gcaaaaacag cgtggcggcg gcaagataca accccaacga accgatatgg gtgataagca
    24301 acgcttttag tgctgaacgc tgcgctttat cgctctggta ataactgatc aacgcccagg
    24361 agcagccgcc ctaatttcaa aaaacaacaa ctgaccgagc agcgtcgagg agagtaccag
    24421 tcccgccatc gcgccgataa acaccagcaa aaatgcgtaa taacgattcg tgccgttatg
    24481 cgggtgttcg cgatttttat ccgtcagata acccgtggag tagatcgtga ccagcaaacc
    24541 gagaaacacc accacaaaca gaatcagcgt actcacgcga tcaatgacca ggccaaacaa
    24601 tgccacatcc cccacgttga ccaacgtcaa cgtgacggca actttatcgg cctgataaaa
    24661 cgcggagatc agcgacaaca tgcacagcgt ggtcagtgcg gcgaacaaaa cccccattcg
    24721 gcggcccgac gttgtggcga aaacgaaacg accagtgcgc cgataaaagg cagcaataac
    24781 gtcgtcagag caagattttc catattccgt cagtgctcct tacagaccgg ttaaccagaa
    24841 gacccatgca agcgcagcaa ggcagaagcc aagccaggtc acatggtgaa tgagtaaaaa
    24901 acgcccgcgt gccagcgtgt tttctgcgat tgaggccagt acaaaaatca gcaaaacctt
    24961 gagcagcgta acgacaagtg aagtcagcag gcaggcgaga gaaagttctt gcgcgcgccc
    25021 aaaggacaga aacagggcca caaacagtga tgccatcacg acctgtttca gccccagccc
    25081 ccatttcgct agcgccagcc cggcaccgga atattcggtc agcgggcctt cctgtaattc
    25141 ctgttctgct tcagcaacat caaagggaat ttttcccatc tcaatgaagc aggcaaaacc
    25201 acaggccagt aacgccagta cggtggttag cggcgagttc cagcccatcg ccagcgtatt
    25261 gctgatcatc tcgatatgcg tggaacctgc tatcagcgcc aataccagca gtgagagaat
    25321 aagcattggt tcgaccagaa tgccgagcgt caactcgcga ctggcaccga ctcccgcaaa
    25381 cggacttccg gtatccagcc cggaaagagc aaagaaaaaa cgaaacaggg caagaagata
    25441 gataagggtg atcagatcgc cgccgcccgc aaaaggggaa acggtaataa acagtggtaa
    25501 ggccatcgcc agcaccagca tgctgctgat taatacccac ggcatcaggc ggaacatcag
    25561 acctgaagat gtcggcgcaa cttcctggcg tttaaacagt ttgtggatat cgcgataatc
    25621 ctgccagatc cccggcccgc ggcgggagtg catacgcgcg cgtatctgcc gggaaataca
    25681 cgtaaaaagt ggggttagca tcagcagaat cacggcctgt gctaacgcaa aaatgaccag
    25741 atatccgtcg caaagagttt gtctcatggt gatttcctta gacggcaata gcgattagca
    25801 gcacaaccag tgcggcgacc acatacaggc aatagagacg aaagtcgccg ctttgcagat
    25861 gctgtatttc tttggccagc cgttgggtgg cgctcacgat ggggcggatc acccgctcat
    25921 cccagaaggg ttctgtgctc tgagccctgg cggtgacgtg cgcaagacct ttatttagcc
    25981 tcagcgtagg gtcgagctgt tgtcgtagac gaaatagcgc agaaaagacc acacgcagcg
    26041 gctgcatcac gccattgcct gacggggcca tcgcattttc ccagccgtag ccgcatgccc
    26101 aggcatctcc tgtgcgacga aacgctgcgc ggcgcgaacg acaccacagc cagtaaaggc
    26161 caggcagcaa aggcatcgcc agtagtaaca gcaacagtaa tgaaggagtg acccgtgtat
    26221 gaaacgtgcc gggtacaaat gctattccgc tggcgacagt gacgggaggg gtattggtaa
    26281 acgcatgggc aatatgcatt atcttcggtg ccagccaact ggcactaagc gcaattaata
    26341 cgcagagtgc cgcgagcagt agcatggcga cgatcatcgt atttggcact tcctgtgcct
    26401 cttcagcgtg tgtactgcgc ggcgcaccac agaaagtaat accgtacatt tttacgaagc
    26461 acattactgc cagcccacca gtgactgcca gcattaccat agcaatagga cccgcaagtt
    26521 gtagcgctac ggcttccaca cgacttagtg agaacagcga ctgccaggtg taccattcgc
    26581 tgataaaacc attcagcgga ggaatggctg atatcgcgag gcaaccaatc aggcatgctg
    26641 cggctgtcca cggcatccgt ttcgccagtg cccccatttt ttccatgtcg tgggtatgca
    26701 aacgcgaaat aatcgcgccc gcgccgagaa atagcagccc tttgaacagc gcatggttga
    26761 gcagatgaaa cagtgcgccg agcagtccaa ccacggtgag cagcgggtcg tgcagtgaca
    26821 gaccgaccat cgccacaccg actgccagca aaataatgcc gacgttttcg acggtactcc
    26881 aggccagcag ccgtttgata ttctgttccg ccagcgcata tagcacgccc aggagcgcgg
    26941 agattgcgcc gatcgccatc accagaatgc cccaccacag aggcaaaccc gtttgcgcca
    27001 gcagatccat cgctactttc aggatgccga aaataccgat tttgaccatt acgccagaca
    27061 tcaacgccga agcgtgcgat ggtgcggcag ggtgagcgcg cggcaaccag ctgtgcaacg
    27121 gcatcatccc ggctttcgcg ccaaaaccga aaaaggccag cagaaacacc gccgacgcca
    27181 gccccggaga aagtgaaagc gtgcggaaac tggcaaaatc gaggctgccg ctttcgcgcc
    27241 ccatcagcaa gaaggcgatc ataatcagca ccgatccggc gtgggcgata aaaaagtaga
    27301 gcatgccagc gttgatcgac gttttatcct gcctggcaat gaccagaaac caggaagaca
    27361 gcgacatcat ttcaaacagc acgatgaacc aaaaagtgtt gtccatcacc agcagggcaa
    27421 ccatcgatgc gatgaaaaga ttcataaaga agcccatcgc cgccgcgcct ttgccctcgt
    27481 attcgcgcat ataagtcaat gaatagagcg aacaaaccac caccagcaag gagataacca
    27541 gcaccataaa tgccgagagg ctatccattc gcagggttaa atcggcaaat tcaaaagggg
    27601 tggcaaaacg ggcgactaaa gttacgcctg catgtaattg cgtaattccg ctgatgatgc
    27661 caatcacgcc gcccaccagc gatgtgatgc cggaaagctt aatagccagt ctgtccagac
    27721 cgagtaaaaa cagcgaagcc agactggcaa acagatagag gatcagcgac caggttaata
    27781 attgcagggc atccattagc gttgctcctg agtgagggaa aggggatcct ccccgccatt
    27841 ggcaaaccag cttgcggcaa ggcgctgttt ttctttcatc tgacgttgca ggctatcgtc
    27901 ggtgatcagc cgtaacgcct gattcgggca agcgcgaacg caggccggac cttctggcaa
    27961 gaaatcacac aggtcgcatt tcaccgcgac ggtctggaca cccggttccc agcgcagcaa
    28021 aggatgttgg ggcggcgcgt tttcttcgcc gtctttgagt gagccctcag cctgaaaaac
    28081 atattgcgca ggggcattca ccggacggct tcctgaagcg ctgattgcgc caaatgggca
    28141 gacctgcagg caaggggctt cctcacagtg atgacacacg acaggggcag tgagtgttga
    28201 cgtcttcgcc agggccaggc gcgggtgttg ctgtaaacct tgcgttttat gcacgtccga
    28261 acaggcagcg agacaggtat tacatcctgt acaccacagt ggttcggcca ccacaaagcg
    28321 gttcatgtcg gctccttgaa aatgatatga aacattgcat ttcatattgc ataaaggatg
    28381 ccagtctgat taactcaatt tattcaataa gttatgtatt aactggattc tgaacggtac
    28441 aaaaatgatg gtcattaacg ttcagcaata aatggattcg acacccgtgt cgcaggggaa
    28501 agtgagaatg tgatcttgat cacgagaagt gaggtgtgct gaccgcaagc gcagccagca
    28561 cggaatggag caaagtaatc aggcgtgttc tttcagccag ttcagcacaa cgtcgtggtg
    28621 attgctggtt ttgaaatcgt caaagacatg ttcgattttg ccatcagcgt caatcaggaa
    28681 gctgatgcga tgaatgccat cgtaggtttt gcccatgaag gacttttcac cccagacgcc
    28741 gaattgttcg cacacctggt ggtcctcatc agacaggagc gtaaagttaa gcagctcttt
    28801 ttccgcaaaa cgggagagtt tttcgggttt atcggtgctg atacccagca catcaacgcc
    28861 cgcttttttc aactcatcca tgttatcgcg taagccgcag gcctgtacgg tacagccggg
    28921 ggtcatggct ttcgggtaga aataaaccag aacacgctgt ccctggaagt cggtcaaatt
    28981 aacttgttct ccgtcttgat ccggcaagct aaatttcggt gcgatatcac cggctttcag
    29041 tggattcatt acttaactcc atcctgttca tcatgttggg aataattgac gacgttaata
    29101 ctgccttgtg cattgagttc tgtacatagg gctttgaacg cttgctcaat atttgctgcg
    29161 tccgcagatg cggggctgtg ggcggttatc tgaatatgca actgcgcagc ccgttcattt
    29221 tcagcaggtt gcgtgcgcga caccagctcc gcaatgttca tatgatgcgt gtcgaaaagt
    29281 gctgtgaagc gttcaattaa atgcggggag tctgccacat cgacctgaac ccagacagat
    29341 gctggcattg gcggacgcgg acgcgccgtc gtgcgcttca tcacgattaa aagatccagc
    29401 tcggcacctt tcaacggtaa cgttgattca atcagagtaa tggcattcca tgaaccggaa
    29461 agcagcatga taaacgtgaa ctcttctccc agcatcgcca ggcgactgtc ttcaatatta
    29521 cagccgcaac tactgacatg acgggtgatg gtgttcacaa ttccagggcg atcggcaccc
    29581 aacgcagtga tcaccagata atgttgcgat gacagtgtca aactggttat tcctttaagg
    29641 ggtgagttgt tcttaaggaa agcataaaaa aaacatgcat acaacaatca gaacggttct
    29701 gtctgcttgc ttttaatgcc ataccaaacg taccattgag acacttgttt gcacagagga
    29761 tggcccatgt tcacgggaag tattgtcgcg attgttactc cgatggatga aaaaggtaat
    29821 gtctgtcggg ctagcttgaa aaaactgatt gattatcatg tcgccagcgg tacttcggcg
    29881 atcgtttctg ttggcaccac tggcgagtcc gctaccttaa atcatgacga acatgctgat
    29941 gtggtgatga tgacgctgga gctggctgat gggcgcattc cggtaattgc cgggaccggc
    30001 gctaacgcta ctgcggaagc cattagcctg acgcagcgct tcaatgacag tggtatcgtc
    30061 ggctgcctga cggtaacccc ttactacaat cgtccgtcgc aagaaggttt gtatcagcat
    30121 ttcaaagcca tcgctgagca tactgacctg ccgcaaattc tgtataatgt gccgtcccgt
    30181 actggctgcg atctgctccc ggaaacggtg ggccgtctgg cgaaagtaaa aaatattatc
    30241 ggaatcaaag aggcaacagg gaacttaacg cgtgtaaacc agatcaaaga gctggtttca
    30301 gatgattttg ttctgctgag cggcgatgat gcgagcgcgc tggacttcat gcaattgggc
    30361 ggtcatgggg ttatttccgt tacggctaac gtcgcagcgc gtgatatggc ccagatgtgc
    30421 aaactggcag cagaagggca ttttgccgag gcacgcgtta ttaatcagcg tctgatgcca
    30481 ttacacaaca aactatttgt cgaacccaat ccaatcccgg tgaaatgggc atgtaaggaa
    30541 ctgggtcttg tggcgaccga tacgctgcgc ctgccaatga caccaatcac cgacagtggt
    30601 cgtgagacgg tcagagcggc gcttaagcat gccggtttgc tgtaaagttt agggagattt
    30661 gatggcttac tctgttcaaa agtcgcgcct ggcaaaggtt gcgggtgttt cgcttgtttt
    30721 attactcgct gcctgtagtt ctgactcacg ctataagcgt caggtcagtg gtgatgaagc
    30781 ctacctggaa gcggcaccgc ttgcggagct tcatgccccg gctggaatga ttttgccggt
    30841 gacctccggt gattatgcaa tcccggtgac caacggtagt ggtgctgtcg gtaaggcgct
    30901 ggacattcgt ccaccagccc agccgttggc actggtttct ggcgcgcgta cccagttcac
    30961 gggcgatacc gcttcattgc tggtggaaaa tggtcgtggc aatactctgt ggccgcaggt
    31021 ggttagcgtg ctgcaggcga aaaactacac catcacccaa cgtgatgatg ctggtcagac
    31081 actgaccacc gattgggtac aatggaaccg tctggacgaa gacgagcagt atcgtggtcg
    31141 ttatcaaatc tctgttaagc cgcagggtta tcagcaggcg gttacggtta aactgctgaa
    31201 cctggaacag gcgggcaaac cggttgcaga cgcggcttcc atgcagcgtt acagcacgga
    31261 gatgatgaac gttatttccg ccggtctgga taaatctgcc actgacgccg cgaacgctgc
    31321 gcaaaatcgt gcctccacca ctatggacgt acaaagtgca gctgatgaca ccggtttacc
    31381 aatgctggtc gtacgcgggc cgttcaatgt ggtttggcaa cgtctgccag cggcgctgga
    31441 aaaagtgggc atgaaagtga ccgacagcac ccgttcgcag ggcaacatgg ccgtaactta
    31501 taagccgctg tctgacagcg actggcagga actgggcgcg agcgatccag gcctggcatc
    31561 cggtgactat aaactgcagg ttggcgattt agataaccgc agcagcctac agttcatcga
    31621 tccgaaaggt catactctga ctcagagtca gaacgacgcg ctggtagctg tcttccaggc
    31681 tgcgtttagc aagtaaaaat acagggctgg aatcatccgg cccttttttc tgatatgata
    31741 cgcaaacgtg tgcgtcggca ggaaaacgcg attttagcgg taattcgcac gaaatttgtt
    31801 tgtcggacgt agttcggata aggcgttcac gccgcatccg acaaaacatc cggcacacca
    31861 gacagcaaaa gattttaaaa cgttaattca cacccaggag tgataaagat gcaaaagcaa
    31921 gctgagttgt atcgtggtaa agcgaaaacc gtatacagca cggaaaaccc ggacctgttg
    31981 gtgctcgaat tccgcaatga tacgtcagca ggggatggcg cgcgcattga gcagtttgat
    32041 cgcaaaggta tggtgaacaa caagttcaac tacttcatca tgagcaaact ggctgaagcg
    32101 ggtatcccga ctcaaatgga gcgtctgctc tccgataccg aatgtctggt gaaaaagctg
    32161 gatatggtgc cggttgagtg tgtcgtgcgt aaccgtgctg ctggctctct ggtgaaacgt
    32221 cttggaatcg aagaaggtat tgagctgaac ccgccgctgt tcgatctgtt cctgaaaaac
    32281 gacgccatgc acgatccgat ggtcaacgaa tcttactgcg aaacctttgg ctgggtgagc
    32341 aaagagaacc tggcgcgtat gaaagagctg acctacaaag cgaacgacgt gctgaaaaaa
    32401 ctgttcgatg atgctggtct gattctggtc gacttcaagc tggagtttgg cctgtacaaa
    32461 ggtgaagtgg tactgggcga tgagttctcc ccggacggca gccgcctgtg ggacaaagaa
    32521 acactggaga aaatggacaa agaccgtttc cgccagagcc tcggtggcct gatcgaagcc
    32581 tatgaagccg tcgcccgccg cctgggtgta cagctggact gatttttctg gttgcttgtt
    32641 tctttagccg gagggtactc cggctaaatg tctttgatac ccccgctaca atacttctca
    32701 ttatctccct ttgacaggaa agtcatacca ggccaatatg tatatacata acgtatatcc
    32761 caaaaagggg gggctatgga aactactgta tttttaagta atcgcagtca ggccgtcaga
    32821 ttaccgaaag cggtcgctct gccagaagac gttaagaaag tagaaattat tgcgattggt
    32881 cgtacaagaa tcatcactcc cgccggtgag tcctgggaca gttggtttga tggcgaaaat
    32941 gtgagtgcgg attttatgga tatccgtgat caacccgcaa tgcaggaaag ggaatcattt
    33001 tgatgctgaa attcatgctg gataccaata tctgtatttt cactattaag aataaaccag
    33061 tacatgtcag agagcggttt aacctgaatt ctggccggat gtgtataagt tctgtaacat
    33121 tgatggagct tatctatggg gcagaaaaaa gtcagatgcc agaacgcaat ttggcggtta
    33181 ttgaaggttt cgtatctcgt ttggtagtgc ttgattatga tagtgctgct gcgacgcata
    33241 ccgggcaaat cagggccgag cttgcccggc agggaaggcc tgttggacct tttgaccaga
    33301 tgatagcggg acatgctcgc agtcgcggtt tgattgtggt taccaacaat acccgagagt
    33361 ttgagcgagt ggcgggaatc agactagaag actggagcta acattctctt ttacttctaa
    33421 cattcgtaaa ttatataacg tggcatgaag ccttaattcc tccgccattt catcccgctt
    33481 tcctgtggta atcttcctct gaaccaccta cgatcatttc tataatgaat atattgttga
    33541 ggtatctatg cgttggcaag ggcgacgtga aagtgacaat gttgaagaca ggcgcaacag
    33601 ctctggcggt ccgtcaatgg gcggtcccgg tttccgtctg ccgagcggta aaggcgggct
    33661 gattttactg atagtcgtgc tggttgcagg ctactatggc gttgatttaa ccgggttgat
    33721 gaccgggcag ccggtttctc aacaacaatc aacgcggtca attagcccta atgaagacga
    33781 agccgcaaaa ttcacctcgg tgattctggc aaccacggaa gacacctggg gacaacagtt
    33841 cgagaagatg ggtaagacct atcagcaacc gaagctggtc atgtaccgtg gaatgacgcg
    33901 caccggctgc ggggctggtc agtccattat ggggccgttc tattgcccgg cggatggcac
    33961 ggtttatatc gatctctcct tctatgatga catgaaagac aaacttggcg cggatggcga
    34021 ttttgcccag gggtacgtta tcgcccatga agtcggtcat catgtgcaga aactgttagg
    34081 catcgagccg aaagttcgtc aactgcaaca aaacgcgacg caggcggaag taaaccgctt
    34141 atctgtgcgt atggaactcc aggccgactg ttttgccggt gtctgggggc atagtatgca
    34201 gcagcaaggc gttctggaaa ctggcgatct ggaagaggcg ctgaatgcgg cgcaggccat
    34261 cggcgatgac cgtttacaac agcaaagtca ggggcgagta gtaccagaca gtttcacaca
    34321 tggcacttct cagcaacgct acagctggtt taaacgtggt ttcgacagcg gcgatccggc
    34381 acaatgcaat acttttggta aaagcattta atttccgggg cagggatggc tgaactgact
    34441 gcgctttaca cattaacagc gcaaatgaaa cgtgaaggga tccgccgctt gctggtgttg
    34501 agcggggaag agggttggtg ttttgatcat gctcttaagt tgcgtgatgc cttacctggc
    34561 gactggctgt ggatttcgcc gcagccagat gctgaaaacc actgttcacc ctcggcgcta
    34621 caaactttac ttgggcgcga gttccggcat gcggtattcg acgcccgcca cggctttgat
    34681 gccgctgcct ttgcagcact tagcggaacg ttgaaagcgg gaagctggct agttttgtta
    34741 ctccctgtat gggaagagtg ggaaaaccaa cctgatgccg actcgctgcg ctggagtgat
    34801 tgccctgacc ctattgcgac gccgcatttt gtccagcatc tcaaacgcgt acttacggcg
    34861 gataacgacg ctatcctctg gcggcaaaac cagccgttct cgttggcgca ttttactccc
    34921 cgtactgact ggcaccccgc gaccggcgca ccacaaccag aacaacagca actcttacag
    34981 cagttactga ccatgccgcc gggcgtggca gcggtaacgg ctgcgcgtgg gcgcggtaaa
    35041 tcggcgttgg cagggcaact catttctcgt attgcgggca gtgcgattgt caccgcgccc
    35101 gcaaaagcgg caacggatgt actggcacaa tttgcgggcg agaagtttcg ctttattgcg
    35161 ccggatgcct tgttagccag cgatgagcaa gccgactggc tggtggtcga cgaagccgca
    35221 gccatacctg cgccgttgtt gcatcaactg gtatcgcgtt ttcctcgaac gttgttaacc
    35281 actacggtgc agggctacga aggcaccgga cgtggttttt tgctgaaatt ttgcgctcgc
    35341 tttccgcatt tacaccgttt tgaactgcaa caaccgatcc gctgggcaca gggatgcccg
    35401 cttgaaaaaa tggttagtga ggcactggtt tttgacgatg aaaacttcac ccatacacca
    35461 caaggcaata tcgtcatttc cgcatttgaa caaacgttat ggcgaagcga gccagaaacg
    35521 ccgttaaagg tttatcagtt attgtctggt gcgcactacc ggacctcgcc gctggattta
    35581 cgccggatga tggatgcacc agggcaacat tttttacagg cggctggcga aaacgagatt
    35641 gccggagcgc tgtggctggt ggatgagggg ggattatctc aagaactcag tcaggcggta
    35701 tgggcaggtt ttcgtcgccc gcggggtaat ctggtggccc agtcgctggc ggcgcacggc
    35761 agcaatccac tggcggcgac attgcgcgga aggcgggtta gccggatagc agttcatccg
    35821 gctcgtcagc gggaaggcac agggcggcaa cttattgctg gtgctttgca atatacgcat
    35881 gacctcgact atctttcggt gagttttggt tacaccgggg agttatggcg tttctggcaa
    35941 cgctgcggtt ttgtgctggt gcgaatgggt aatcatcgtg aagccagcag cggttgctat
    36001 acggcgatgg cgctgttacc gatgagtgat gcgggtaaac agctggctga acgtgagcat
    36061 taccgtttac gtcgcgatgc gcaagctctc gcgcagtgga atggcgaaat gcttcctgtt
    36121 gatccactaa acgatgccgt cctttctgac gacgactggc ttgaactggc cggttttgct
    36181 ttcgctcatc gtccgctatt aacatcgtta ggttgcttat tgcgtctgct acagaccagc
    36241 gaactggcat taccggcgct gcgtgggcgt ttacagaaaa acgtcagcga cgcgcagtta
    36301 tgtaccacac ttaaactttc aggccgcaag atgttactgg tccgtcagcg ggaagaggcc
    36361 gcgcaggcgc tgttcgcact taatgaggtt cgcactgagc gtctgcgcga tcgcataacg
    36421 caatggcaat tttttcactg actccttcaa ttttctccca tggtcattgt gagtcagcgg
    36481 cgtaaaatta atgacatcag ttaaggagtt cgccatgaaa catgaccatt ttgttgttca
    36541 aagcccggat aaacctgcgc aacagttgtt actgcttttc cacggtgtcg gggataaccc
    36601 ggtggcaatg ggggaaatag gcagctggtt tgcgccgcta tttcctgatg cgctggtggt
    36661 gagtgtcgga ggcgcggagc cgagcggtaa tccggcgggg cgtcagtggt tttcggtgca
    36721 gggtattacg gaagataatc gccaggcgcg tgtggatgcc atcatgccga cgtttattga
    36781 gacggtacgc tactggcaga aacagagcgg agtaggggct aacgccacgg cgctaattgg
    36841 tttctcacaa ggcgcgatta tggcgctgga gagcattaaa gccgaaccgg gccttgcttc
    36901 gcgcgtcatt gcctttaacg gacgctatgc gagcctgccg gaaaccgcgt ctacagccac
    36961 gacaattcac ctgattcatg gtggtgaaga tccggttatc gatctggcgc atgcagttgc
    37021 ggcgcaggaa gcgttaatca gtgccggtgg tgacgtgacg ctggatatcg tggaggattt
    37081 aggccatgcg atcgacaatc gcagtatgca atttgccctg gatcacttgc gttataccat
    37141 tccgaaacat tatttcgacg aagcgctaag tggcggtaag cctggcgatg atgatgtgat
    37201 tgaaatgatg taataatagc cgggtaggcc tgataagact gtatcaggca tcatggccgg
    37261 atgtggcgtg aacgccttat ccggccactc agcttacttc ttcggttgat cctttttcgg
    37321 ccagtcgtcg tcatcgtccc acttatcgtt gaaatcacga tgcgggggaa gctccggctt
    37381 gttggcgaga aatttcttat ggtcgacgcg cttgagatct ttaatcacgt tcagcaggac
    37441 gcctaccaaa aagacaatca ccagaatcca ccaatattta gccagccagt ccatgcttat
    37501 ttcctcttgc agaactactc atcaggcgac gagctgttcc atgatacgtt gatacatacg
    37561 ggcaagtagc tgcaggtcgg cagcgttcac acattcatta attttatgaa tagtggcatt
    37621 gaccggcccg agttccacca cctgcgcccc catgcgagca ataaagcgcc cgtcggacgt
    37681 tccgcctgtg gtcagtagct gcggtttaat ttcattatag tgctcaaccg cgttaacgac
    37741 cgcatccacc agtttaccgc gcgcggtcaa aaatggctgc ccggaaagcc accaatccac
    37801 cgtatagcgc agttgatgtt tttcaagcag ggcaagtacc tgcgctttga tcatctcatc
    37861 cgtcagttcg gtgctgaagc ggaagttaaa ctgcacaaac agttcgcccg gaataacgtt
    37921 gttactgccc gttcccgcct gaatattggc aatctgcata ctggtcgccg ggaagaattc
    37981 attgccctga tcccactcaa tagccactaa ttcattaagg aaaggtgctg cgcgatgtac
    38041 cggattgtca gccagatgtg ggtaggcaac atgcccctga acgccatgaa tggtgaggtt
    38101 gcaggttaat gatccgcgac gaccattttt caccacatca cctaccactt cgatactcga
    38161 cggttcgccg accaggcagt aatcgagacg ctcattacgt gccattaacg cttcgacgac
    38221 ttttaccgta ccgttgtggg cactggcttc ttcatcagag gtgatcagaa atgccagtcg
    38281 ccccgtatgg ttgggatgtt gtgcgacaaa acgctctgct gctaccacca tcgccgccag
    38341 cgagcctttc atatctgccg cgccgcgccc gaataacatg ccgtcacgaa tggtcggttc
    38401 aaacggcgga ttgatccaac gatcggcgtc gccaggcggc accacgtcgg tatgcccggc
    38461 aaaggctaac gtttcacctt gcccacgcca tgcccaaaaa ttctgcgtat cggcaaagtc
    38521 catgcgttca acggtaaaac cgatcgcctg caaacgttca atcaacaaag cctggcatcc
    38581 tgcgtcatca ggactcaggg aagggcggcg aataagctgt tgtgtcagct caataaccgg
    38641 gcacgacata gactacacct catggaaaaa ttgctgataa ctggaatcac tgaaacccag
    38701 cagcataggc ttaccaggcg cgcagagcaa tggacgtttg ataattgcag gcatttcagt
    38761 cattaatgcc gccgcagagg ccgcatcggt gattttattg cgggtggttt cgtccagttt
    38821 acgccaggtt gtaccacggg tgttcagtaa cgcttcccag cctaattcgt tgataaaacc
    38881 gttcaataat tcgctgtcca gcccatcgac gcggtaatca tgaaaacgat agtcgatgtt
    38941 attggcttcc agccaacggc gagccttttt aatggtgtca caatttttga tgccgtaaag
    39001 tgtaaccata atgaatcctt tttcgatcta tgagaataaa tactcattta agggtgaaaa
    39061 cattttgcct gcaaatgatt atcaggaaat ataacatt
//
LOCUS       DAZGSP010000044        37944 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZGSP010000044 DAZGSP010000000
VERSION     DAZGSP010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.044"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            6..81
                     /locus_tag="ACHK74_002406"
     tRNA            6..81
                     /locus_tag="ACHK74_002406"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHK74_002407"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHK74_002407"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(468..1226)
                     /gene="yhdZ"
                     /locus_tag="ACHK74_002408"
     CDS             complement(468..1226)
                     /gene="yhdZ"
                     /locus_tag="ACHK74_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP0221216.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMERGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(1234..2337)
                     /gene="yhdY"
                     /locus_tag="ACHK74_002409"
     CDS             complement(1234..2337)
                     /gene="yhdY"
                     /locus_tag="ACHK74_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP0221217.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2347..3528)
                     /gene="yhdX"
                     /locus_tag="ACHK74_002410"
     CDS             complement(2347..3528)
                     /gene="yhdX"
                     /locus_tag="ACHK74_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP0221218.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3596..4621)
                     /locus_tag="ACHK74_002411"
     CDS             complement(3596..4621)
                     /locus_tag="ACHK74_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0221219.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGLVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5052..5273)
                     /locus_tag="ACHK74_002412"
     CDS             complement(5052..5273)
                     /locus_tag="ACHK74_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221220.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNICMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5526..8630)
                     /gene="acrF"
                     /locus_tag="ACHK74_002413"
     CDS             complement(5526..8630)
                     /gene="acrF"
                     /locus_tag="ACHK74_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFP0221221.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPTSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNSDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8642..9799)
                     /gene="acrE"
                     /locus_tag="ACHK74_002414"
     CDS             complement(8642..9799)
                     /gene="acrE"
                     /locus_tag="ACHK74_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFP0221222.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            10198..10860
                     /gene="acrS"
                     /locus_tag="ACHK74_002415"
     CDS             10198..10860
                     /gene="acrS"
                     /locus_tag="ACHK74_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFP0221223.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(10863..11042)
                     /gene="yhdU"
                     /locus_tag="ACHK74_002416"
     CDS             complement(10863..11042)
                     /gene="yhdU"
                     /locus_tag="ACHK74_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFP0221224.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(11126..12010)
                     /gene="yhdJ"
                     /locus_tag="ACHK74_002417"
     CDS             complement(11126..12010)
                     /gene="yhdJ"
                     /locus_tag="ACHK74_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP0221225.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDAASHYSAEELAKVKKRKTG
                     NRSKRCRVSEVDPDLIAK"
     gene            complement(12096..12392)
                     /gene="fis"
                     /locus_tag="ACHK74_002418"
     CDS             complement(12096..12392)
                     /gene="fis"
                     /locus_tag="ACHK74_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFP0221226.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(12418..13383)
                     /gene="dusB"
                     /locus_tag="ACHK74_002419"
     CDS             complement(12418..13383)
                     /gene="dusB"
                     /locus_tag="ACHK74_002419"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFP0221227.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(13712..14593)
                     /gene="prmA"
                     /locus_tag="ACHK74_002420"
     CDS             complement(13712..14593)
                     /gene="prmA"
                     /locus_tag="ACHK74_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFP0221228.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(14605..16056)
                     /gene="panF"
                     /locus_tag="ACHK74_002421"
     CDS             complement(14605..16056)
                     /gene="panF"
                     /locus_tag="ACHK74_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFP0221229.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(16046..16288)
                     /gene="yhdT"
                     /locus_tag="ACHK74_002422"
     CDS             complement(16046..16288)
                     /gene="yhdT"
                     /locus_tag="ACHK74_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFP0221230.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(16397..17746)
                     /gene="accC"
                     /locus_tag="ACHK74_002423"
     CDS             complement(16397..17746)
                     /gene="accC"
                     /locus_tag="ACHK74_002423"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFP0221231.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(17757..18227)
                     /gene="accB"
                     /locus_tag="ACHK74_002424"
     CDS             complement(17757..18227)
                     /gene="accB"
                     /locus_tag="ACHK74_002424"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFP0221232.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(19205..20179)
                     /gene="yhdH"
                     /locus_tag="ACHK74_002425"
     CDS             complement(19205..20179)
                     /gene="yhdH"
                     /locus_tag="ACHK74_002425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFP0221233.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            20331..22271
                     /gene="csrD"
                     /locus_tag="ACHK74_002426"
     CDS             20331..22271
                     /gene="csrD"
                     /locus_tag="ACHK74_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFP0221234.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            22576..23619
                     /gene="mreB"
                     /locus_tag="ACHK74_002427"
     CDS             22576..23619
                     /gene="mreB"
                     /locus_tag="ACHK74_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFP0221235.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            23685..24788
                     /gene="mreC"
                     /locus_tag="ACHK74_002428"
     CDS             23685..24788
                     /gene="mreC"
                     /locus_tag="ACHK74_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFP0221236.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            24788..25276
                     /gene="mreD"
                     /locus_tag="ACHK74_002429"
     CDS             24788..25276
                     /gene="mreD"
                     /locus_tag="ACHK74_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFP0221237.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            25285..25878
                     /gene="yhdE"
                     /locus_tag="ACHK74_002430"
     CDS             25285..25878
                     /gene="yhdE"
                     /locus_tag="ACHK74_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFP0221238.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            25868..27337
                     /gene="rng"
                     /locus_tag="ACHK74_002431"
     CDS             25868..27337
                     /gene="rng"
                     /locus_tag="ACHK74_002431"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFP0221239.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            27405..31205
                     /gene="yhdP"
                     /locus_tag="ACHK74_002432"
     CDS             27405..31205
                     /gene="yhdP"
                     /locus_tag="ACHK74_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFP0221240.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADNTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKLLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSMAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            31361..32806
                     /gene="tldD"
                     /locus_tag="ACHK74_002433"
     CDS             31361..32806
                     /gene="tldD"
                     /locus_tag="ACHK74_002433"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFP0221241.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(32934..33863)
                     /gene="aaeR"
                     /locus_tag="ACHK74_002434"
     CDS             complement(32934..33863)
                     /gene="aaeR"
                     /locus_tag="ACHK74_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFP0221242.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            34046..34249
                     /gene="aaeX"
                     /locus_tag="ACHK74_002435"
     CDS             34046..34249
                     /gene="aaeX"
                     /locus_tag="ACHK74_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFP0221243.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            34257..35189
                     /gene="aaeA"
                     /locus_tag="ACHK74_002436"
     CDS             34257..35189
                     /gene="aaeA"
                     /locus_tag="ACHK74_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFP0221244.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            35195..37162
                     /gene="aaeB"
                     /locus_tag="ACHK74_002437"
     CDS             35195..37162
                     /gene="aaeB"
                     /locus_tag="ACHK74_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFP0221245.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            37254..37526
                     /gene="yhcO"
                     /locus_tag="ACHK74_002438"
     CDS             37254..37526
                     /gene="yhcO"
                     /locus_tag="ACHK74_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFP0221246.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(37582..37845)
                     /gene="yhcN"
                     /locus_tag="ACHK74_002439"
     CDS             complement(37582..37845)
                     /gene="yhcN"
                     /locus_tag="ACHK74_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFP0221247.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         9362 a         9566 c         9828 g         9188 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aataaaacat aaggctcagt cggaagactg ggccttttgt tttatttgat gtttgtcggt
      301 gaacactctc ccaaccagga caaatccgcc gggaacggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcaaaa
      421 aggccatccg aaaggatggc ctttttgctt ttcgaactaa cattcaatta atggattacc
      481 tgcgataaaa atgccctcgt acgctctgat ttaggatgcg taaaaaattc atcaggtgca
      541 gcttgctcca ctatttcccc acgctccata aaaatcaccc ggtcagcgac ggttcgcgca
      601 aaccccatct catgcgttac acacaacatt gtcatacccg attgcgccag cccaatcatc
      661 gtatccagca cctctttcac catctccgga tcgagcgccg acgttggctc atcaaacaac
      721 ataattttcg gcttcataca cagcgaacgc gcaatggcaa cgcgttgctg ctgaccacca
      781 gaaatttgtc cgggaaactt atgcgcatgt tcggcaattc tcacccgctc aaggtaatgc
      841 atcgccagag cttcagcctc tttctttggc atcttgcgta cccaaatcgg tgccagggta
      901 cagttctgta gaacggtcag atgagggaag agattgaaat gctgaaagac cattcccact
      961 tcctgccgga cgcgctcaat attgcggata tcttcattaa gttcgatgcc atctaccacg
     1021 atccgtccct gttgatgttc ttccagatga ttaatacaac gaatggttgt cgatttaccg
     1081 gaacctgaag ggccacacag aacgatccgt tctcccggtt gcacggttaa atttatattt
     1141 ttcaaaacat ggaattgtcc ataccattta ttgacgtttt ccagcgtaat catcgcgtta
     1201 gcaggttgca gtaaaatttg gctcatagtg tcctcaatgc ggtgtacgcc cggtgttaaa
     1261 acgtttttcc agatgctggc tatagcgcga catgctgaaa cagaagatcc agtagatcag
     1321 cgcggcgaaa acataccctt ccgtcgacat acccaaccag gtgggatcaa cggttgcctg
     1381 ctgaacgcta ctgaaaagat cgaacaaccc gataatgatc accaggctgg tatctttgaa
     1441 gagtgcgatg atggtattta ccagcccagg aattaccagc ttcaacgcct gtggcagaat
     1501 aaccagcccc tgagttttcc agtaacccaa cgccagcgac tctgccgctt catattgccc
     1561 tttaggcagc gcctgtaatc cgcctcgcac gacttccgca acatatgctg actgaaacag
     1621 gatcacgcca accagcgcgc ggatcagttt gtcgatattg gtgccttctg ccataaacaa
     1681 aggcagcatg accgaagaca taaacaatac ggtaatcagc ggtacgccgc gccagaactc
     1741 gataaaaatg accgataaga tacgcacaat cggcatatgg gagcggcgac ctaacgccag
     1801 taagatcccc cacggtagcg cccccgcaat cccaacggat gcaataatta aagttagcgt
     1861 cagcccgccc cattgccggg tttcaacccg ctccagacca aaaaaaccgc catacatcag
     1921 ccaccagaca atcagtgggt aaatcaccgc ccagaccgca atatagcgac cgcgatgcgg
     1981 gagtattttc cagaacattg gtgcgattga cacaaggcca atcagtaatg ccaggttaat
     2041 tcgccagcgt tggtcgtgtg ggtaaagtcc atacataaac tgaccaaatc gttcgtggat
     2101 gaagacccaa caggcaccgg cttttgtgca gtcggcacgc gttgagccaa cccaattagc
     2161 ctgcaaaaat gcccagttca gcaacggtgg gatcagttcc cacatcaacc atatgcagcc
     2221 aatagtcagc aggctattgc tccagctgga gaacagattt tttcgcaccc acaccatcgc
     2281 gcggcttgag ttatggctcg ctgggcgcga gggctgagac agcaatactt ttgtcatcat
     2341 gactccttag cgttcaacga tcgcgatgcg gcggttatag atattcatca gcagcgagat
     2401 agtcaggctg ataatcagat agaccgacat ggtcatggct atcgtctcga tggcttgccc
     2461 cgtctggttc agcacggtgc cagcaaacag cgaaaccata tcgggatagc caatagcggc
     2521 ggcaagagag gagtttttga cgatgttgag atactggctg gttaacggcg gaataatcac
     2581 tcgcagtgcc tggggaataa tgacctggcg tagcgtaacc gggttgggta atcccagtga
     2641 ccgagccgct tcatgttgac cataaggcac tgcctggatc ccagcgcgga taatctcggc
     2701 gataaatgca gatgtataaa ccgaaagtgc cagcgttaag gctgccagtt caggaattaa
     2761 aaccatcccg ccgcggaagt taaagcctcg tagggctggg acatcccagt gtagtgctgc
     2821 gccaaaaagc cattgcgcca gtaaaggcaa accaatgatc aacactgcgg cgatcggcca
     2881 ggtgcggcgc agttgtccgg tctttatctg gtatgtttta ttaaagcgga atagcccaac
     2941 agaaaggact atagccataa caacagccag gataaacgca ataaatccgt ctcccagctg
     3001 cggtgacgga atataaagcc cacgattgct caaaaaggcc agatcaaacg cgctcacagc
     3061 ctggcgtggt ccgggcaaat tgcgcaacac ggcaaagtac cagaagaaga tttgcagcag
     3121 cgggggaata ttacggaaga tctcaatata aattgtggat agttttcgta gcagccagtt
     3181 atccgaaagt ctcgccagac cgataaagaa gcctaaaaca gaagcgaaca caatacacaa
     3241 tgcagaaacc agtagcgtat tgagtaagcc gacaataaaa acgcgtccgt aggtgtcgcc
     3301 ctgctggtaa tcgatcaaat gctggacaat accgaagcca gcgccgcgat ccagaaaggc
     3361 aaaacctgaa gtaatgccac gattattgag attcgttaca gtgttgtgaa ataaccaacc
     3421 aacaatgccg acaacagcaa caacggcaag gatctggaat aaccaggcgc gaaccgtagg
     3481 gttggcaaaa gagagtgagc ctttaacggt tgagcggcga tgagacatag aaacctcgga
     3541 aagcataacg ctgaagagag cgccgccgta gcgacgcccg ttacctcaca ccgacttaac
     3601 gcacgggcgg cgcgtactga ataccgccgt tattccagag attattttgc ccacgtttaa
     3661 ttttcagcgg gctttctgaa cctacgttac gctcgaaaat ttccgagtag ttacccacct
     3721 gtttgatgat gttataggcc catttattat ccagcttcag atccttgccg taatcgcctt
     3781 ctttacccag cagatgtgcc atatcaggcg ttgctggatt agccgctttt tcatcgacgt
     3841 tctgggaatt gatgcccatc tcttcagcat tcagcatggc gaaaagcgtc cagcgtacaa
     3901 tcgagaacca ttcatcatcg ccacgacgaa ctaccagccc aagcggttct ttagagataa
     3961 cctccggtaa gacaatccat tcagctgggt tgcttaattt gatgcgcagg gcatacagtt
     4021 gcgactgatc cgaggccagc gtatcgcagc gaccagattc cagtgccttc gctgattcgt
     4081 cagagcgatc gaaagtcacc ggtgtgtact tcatattgtt tgccttgaaa tagtcggcaa
     4141 cgttgagttc ggtatcagta cccgcctgaa tacagacggt agcgccatcc agttctttcg
     4201 cgctttttag ccccgcttta tcgtgcgtca ggaagccaat gccgtcgtaa taagtgacgc
     4261 cggtaaatgc cattcccatc ccggcatcgc gagatgaagt ccaggtcgta ttacgggaga
     4321 gcaaatccac ttcccctgac tgtaaagcgg tgaagcgttc ttttgctgtg agtggggtat
     4381 atttcacttt cgtgtcgtca ccaaatacag cagcggcaac accacgacaa acatcaacat
     4441 caatacctga aaacttaccg tctgcatcgg cataagagaa cccaggtaat ccatcactga
     4501 tcccgcattg cacaaaacct tttttctgaa cggcatcaag cgtcgcgcca gcatgcgcct
     4561 gatttgcaac ggcaagcagc acgctggcgg cagccagtgt ggctatcatc atctttttca
     4621 taatgcttcc tgtgaggcga aaattatcgt tataggacgt ttgggaaacg tcttttacct
     4681 gtctgtggcc atcctcttat aagcaaaagt agtgccagct tttgcgcccg atttcgcgtc
     4741 gcaataagct gagtgtagac aggaaataat aaaaacagcg ccccgcaatg gtgcgaaaat
     4801 gaaacatgcg ccacattttg gcgcaaaata tttacgcgtg ttttactaag cagaataatg
     4861 attgttagcg tgagtatatt tctggtggga agtgtgaaag ttaaattgat gcgttatttc
     4921 gaaagcagcg aaataacgcg atataacatg ctaaatcagc agagaattag ccagcagaat
     4981 atagcaatat tttatcttat gtaaagatac tgtatcgtta aacagagcgc gaaactccgc
     5041 gccctgttca gttatttcga cagagcgatg atccccatcg tcaggccagc aacagccgct
     5101 gccccgccag caatagcccc agccgtttcc cagttatcct gagtcgtgcc tttcatacag
     5161 atattggtgt gaaccagacg accctgatcg tcataaaccg gaacgcaggg cgaatcgtga
     5221 gcacagcccg ccagcaatgc ggtgagcaat gccacaggaa ttaatctttt catggtgtta
     5281 cctcagtaat aaacgctaac gcttaataag ttaactctct tattaacgag ctgaatgcca
     5341 ttttatcacc ggaggttata aatcttgcgg aatgaataat gacaaattat gtgaattgta
     5401 aaaatagtga agggagcggg aactatagaa agcttttaaa gagtgagcag aagaatatct
     5461 tattaatttt tatgggtttc actggaaata ataaaggcac ccgaaagcgc ctttatgttt
     5521 ctgatttatc ctttaaagca acggcggatc accacaaaga acaccggtac gaagaagatt
     5581 gccagcaacg ttgcagagac cattcctccc attaccccga tacccacagc gttctgcgcg
     5641 ccactgccgg caccgttact gatagctagc ggtaatacgc cgagaataaa ggcgagagag
     5701 gtcatcagga taggacgcag acgcatacgt actgccatca gtgtcgcttc aacaacacct
     5761 ttaccctctt tctccatgag atctttagcg aactcaacaa tcaaaatagc gtttttggcc
     5821 gacaagccaa ttgtcgttag caagcccacc ataaagtaga cgtcattttt ttgattaaag
     5881 agtgttgccg ccagtagcac gccgacaatc cctaacggca ctaccaacat aaccgagaca
     5941 ggaattgacc agctttcata gagtgcagca aggcacagga aaacaaccac aaaggaaatt
     6001 gctaccagag cgggagcctg gtttcccgat aagcgttcct gatacgacat acccgtccag
     6061 tcataaccaa tgcccgcagg taattttgac gcaaggtttt ccatcaacgc catggcatcg
     6121 ccggaactgg ttcctggcgc ggcttccccc tgaatctcca ttgacggcag accgttgtag
     6181 cgttccagtc gcggagagcc atacacccaa tgtgaagtgg taaaggccga gaatggcacc
     6241 atttcgccgt tggcgctgcg gacataaagt ttatcgacat cttctggcag catacggaat
     6301 ttggcatccg cctgaacata cacctttttc acgcgaccac ggtcgatgaa gtcgttaacg
     6361 taagtcccac ccaacgccgt tgaaatggtc tgattgatgt cagaaagtga aacacctaat
     6421 gcctgcgcct tttcctggtc aacttccagt ttaaactgcg cggtgtcttc caggccatta
     6481 ggacgcacgc tgactaagct ggcaggatgt tgcgccgcca tgccaagcaa ctggttacgg
     6541 gcctgggtca gggcatcgtg acccagccca gcctgatcaa ttaactcaaa gtcgaaaccc
     6601 gttgccgtgc ccagttcaac aatggctggc atattgaatg gaatgacaaa accgtcgcgg
     6661 atcttgccca attccatttt ggcacgatgg attaccgctt ccgcactgtt ttcatcacta
     6721 ttacgctctt cccacggttt cagactgacg aaggccatac cggcgttttg tgcctggccg
     6781 ctgaagctaa agccgttaac cgtaaagaca ctttcaacgt tcgctttctc gttcttcaga
     6841 tagtaatccg tcacttgatc caacactttt tgcgtccgct cttgcgtcgc gccagcgggt
     6901 aactgaatca tggtcagaaa gacaccctga tcctcttcag gtaagaagga agacggaaga
     6961 cgtaaaaaca acaccaccat tcctgcaaca atcagcgcat agatcagtaa atatcgtcct
     7021 gtggaaccga ggattttgcc gacgctgttg gtgtagtggt taacgctatg atcgaaggtg
     7081 gtattaaacc agccgaagaa accaccctta ttttcgtgat gctcagctga ggtgggtttg
     7141 agcagtgttg cacataacgc aggggtaaga atcaatgcca ccagaacaga aagcgccatt
     7201 gccgaaacga tggtgataga gaactggcga taaattgccc cagtagaacc gccgaagaat
     7261 gccatcggaa taaataccgc tgacagcacc atcgcgatac ccaccagtgc gccctgaatt
     7321 tgcgacatcg atttttccgt cgcttctttt ggcgggagct tatcctccat catcacgcgc
     7381 tcgacgttct ccaccaccac tatcgcatca tcgacgagca gcccgatggc aagcaccatc
     7441 ccgaacatcg ttagtgtgtt gatggagtaa ccaaaagcgg cgaggatggc aaacgtccct
     7501 aataacacca cgggtaccgc aatggtgggg atcagcgttg ctcgcatatt ctgcaagaac
     7561 agatacatca ccaggaacac cagcataatg gcttcgaaca gcgtttttac cacttcgtga
     7621 atagaaagct ggacgaatgg cgtggtgtca taagggtaga gaaccttcat tccctgcggg
     7681 aagaatggct gtaattccgc cagttttgcc ttaatggctt tcgcggtatc gagagcattc
     7741 gcgccggttg ccagcttaat ccccaggccc gccgccggtt ttccgttgat acgagcgata
     7801 acgttatagt tttcaccgcc aagttcaact cgtgcgacat ctttcaggcg taccaccgag
     7861 ccgtcactgt ttacgcgcag ggttactttg ccgaattctt ccggattttt taaccgcgtc
     7921 tgagcaataa ttgaggcgtt caactgttgc cctggtaacg ctggcgttcc gcccaactgt
     7981 ccggcagcga tctgatcgtt ctgtaccttc aactggttaa tcacatcaac cggtgtcagt
     8041 ttatatttgt ttagcagatc ggcgtccagc cagatacgca tcgcatactg tgcgccgaaa
     8101 agctgtacgt caccgacgcc attcagacgg ctaagcgtat ctttaacgtt agaggccaca
     8161 tagtccgaga tatcgtcctg tgtggtgtct gggttatcag agacaaagcc cgccaccatc
     8221 aaatagctgc tactggactt ttcaacactg atcccctgct gctgaacctc ctgcggcagc
     8281 aacggcgtgg cgagctggag tttgttctgc acctgcactt gcgcgatatc aggatcggtc
     8341 ccggactgga aggtaagggt aattgtcacg ctaccggcgg aatcgctggt ggaggacata
     8401 tacatcaggt tatcgatacc gttcatattc tgttcgataa cctgcgtcac cgtatcctgc
     8461 acggtctgcg catcagcgcc cggatagttt gctgaaacag aaaccgcagg cggtgcaatg
     8521 gttggatact gagcgacggg taattgtagg atcgccagtg cgcccgccat catcagaata
     8581 atggccagca cccatgcaaa tatcggtcgt cgaataaaaa agtttgccat gtcagattac
     8641 cttacttcga tgcagtatct gcgggggtat cggtagtggc tttaacctgc tctcccggac
     8701 gcgctttttg caggccgctg acaatgactt gatcgccaga tttcagacct tcgctaatca
     8761 accatttatc gccaatcgcc tgactggcaa cgacagggcg cgcttccacc tgacttttat
     8821 cgttaacaat cagcacggtt gcatcaccac gcggtgtgcg gctaacgcct tgttgcggga
     8881 taagaatggc gtcaggttgg acgccttcat caatccgtgc gcgcacaaac atacccggca
     8941 aaagcgtatg ttgcgggtta gggaagacgg cacgtagggt tatggagcca gtgctttcat
     9001 caacggtcac atcagagaat tgcagcgtac ctttcagggg ataggtttga ccgttttcca
     9061 tgaccaactc tacgttgctg gtggcgtttt ccttatgcaa atttccttgc tctacggatt
     9121 gcttcagcct cataaaatcg ttgctggatt gggtcacatc aacgtagata ggatcgagct
     9181 gctggacagt cgccagttca gtcgtttgcc cattagtgac aagagcgcct tcggtcacgg
     9241 tcgatttgcc gatacgtccg ctaattggcg cagtcacttt ggtataagcg agattgatgc
     9301 gagcgctttc gactgtggct tttgcggcaa tcacggcggc atcggcctgg cgagcatcag
     9361 caatggcctg gtcgtactcc tgctggctga tgtatttcgt acccacgagc ggaacgtaac
     9421 gttttaccgt caaatgcgcg atggcggcgg cggcttcact tttcgccagt tcgcctttcg
     9481 cgctgtcata atttgcctga taggtcgcgg gatcgatctg gtacagggac tggcctgctt
     9541 gcacatcgct gccttcagtg aaattgcgat tcagtacgat cccgctaacc tgtgggcgaa
     9601 cttcggctat acggtaagca ttggtgcggc ctggtaattc agtcttaact tctaacgggg
     9661 ccgtttttac aatatgaacg gtaacctgcg gttcaccgac gtgagctttc tcttctccct
     9721 tatcgttaca accggcgatt aaagccgcgg agatcagaat aaaggagggc aggaggaaaa
     9781 acctggcatg tttcgtcatt actattcctc aaaaaaccaa aagcgcgtta tttacccaaa
     9841 aaggcaacgc gttaactcgc agaaagaaaa atacagttcg ctatcctaca aattatcatt
     9901 cgtcgatgta aggaatagtt atgaatacag gcgtctcaag gcacataaac acaaaaaaag
     9961 attaatattc tactgtttta ttttgacgcg ggttgaaaga ggcggaatta aaacatcgta
    10021 aattgaaata tatattgatg tagtgaatgt atcttaggta aataatatat attatttatt
    10081 taacgattta tagcaactca atattagcct ctcgtataaa tacacattag gtgatagatt
    10141 aaccttcgct attttctcac tctgtgtcga atatatttat ttcctgaata attaatcatg
    10201 gcaaaaagaa ccaaagccga agctctgaag acccggcaag aactgattga aactgccatc
    10261 gcccagtttg cgcagcatgg cgtaagcaag acgacactca acgacattgc cgacgctgct
    10321 aacgttacgc gtggcgctat ctactggcac ttcgaaaaca agactcaact gtttaatgag
    10381 atgtggttgc aacagccttc attgcgagag ttaatccagg atcacttgac ggctgggtta
    10441 gagcatgacc catttcaaca attgcgtgaa aaattgattg tcggcttgca atatattgcc
    10501 aaaattcccc gccagcaggc gttgctgaaa atcttatatc acaaatgtga atttaatgat
    10561 gagatgctgg ccgagggagt gatacgcgaa aagatgggct tcaatccgca gactctccgc
    10621 gaagtattgc aggcgtgtca gcaacaaggt tgtgtagcaa ataacctcga tttagatgtt
    10681 gtgatgatta ttattaatgg tgccttcagc ggaattgttc aaaactggtt aatgaatatg
    10741 gcgggttatg atctttataa acaagccccc gctctggtcg ataacgtatt aagaatgttc
    10801 atgccagatg aaaacataac gaaattaatt catcaaacga atgaattaag tgtcatgtaa
    10861 attcacattc tgtcctgaaa attcagttcg tcacagttaa ccagttttag tggattaact
    10921 gagttcatat tgcggcattt gtctgtttct gttgccagca gttcaatcca ctgcatcagt
    10981 aaagtatcaa acagaaaaac gaagacagcg aaaacgacga gccaccaata cttgcgaatc
    11041 attacctgag cctgtgtatg tttaaccaag tctggcagga aatatacacg aaatgaataa
    11101 tctgaaaacc aggcttacac ttcctttact ttgcaatgag atcggggtca acttcgctaa
    11161 cccgacatcg ttttgaccgg ttgcccgtct ttcttttttt cactttcgcc agttcctccg
    11221 cggagtaatg tgacgcggca tccagtcgtc gaagccccat tttgatgtac tcgctgttga
    11281 tctcaatacc aatgaatttt cgcccgctgg cgacggctac ggcaccggta gtaaagctac
    11341 cggcaaacgg gtcgagaacg atatcgcctg ggttggaaga ggcgagaata atgcgtttca
    11401 gtaaggcttc cggtttttgc gtcgggtggt tctcatattc atccatcaaa taacgcacgc
    11461 gcggaaaatc ccagacgtta cccggtactt tttgatgatt gtatggctgt ggaggatttt
    11521 tgcgataatc gatcaacgcg cgctgcgatc cggttttggc ttctaccaga atagcatcac
    11581 cgttgaatgt gtagttcttt gcgtctttca ccatcatcag gatgggttcg tacatggagc
    11641 cgtagtgttt tttcgcctgt actccagaac tgtcatatga ccagacgatg cgacttttga
    11701 tggtaaaaag cttgcggcac tggagatcga taaagggcat gttttccgtg ctgttcatga
    11761 tgtacatgct gccctgcttt ttcagaacgc ggtggcactc tgcaatcact tcaaacagcc
    11821 agtcgataaa cagatcttct ttccaggctt cgatcagacc atcaaaattt ttaccgatgt
    11881 tatatggtgg gtcggcaaag atcagatcga cactttcggc ggggatcttt ttaagttcgg
    11941 caagcgcatc accgtgaata atggtcttag cttcattacc aaaccgggtc ggttcacatc
    12001 ctgttctcat ggtcactccc tttgtgacac ctataaaaaa ggcgcttccc catgccgagt
    12061 agcgcctttt taatcaagca tttagctaac ctgaattagt tcatgccgta ttttttcaat
    12121 tttttacgca gcgtaccacg gttgatgccc atcatcagcg cagcacgggt ctggttacca
    12181 cgggtgtatt gcatcaccat gtccaacagg ggctgttcta cttcagccag taccagctca
    12241 tagaggtcat tcacatcctg accattcagt tgagcaaaat agttcttcag tgcctgttta
    12301 accgagtcac gcaggggttt ttgggttacc tgatcctgag agttaacggt agaaacggtc
    12361 agtacgtcag aatttacgcg ttgttcgaac atagttctgt cagctcttta tttctgttta
    12421 cgcaaaattt tcgaagtatg cctccaacgc ctccagctgt tcgctggcat cctcaatggc
    12481 gttgaatgtg cgccgaaact ggtcatttgg agcgtgttcc tggagatacc aggaaacgtg
    12541 tttacgtgca attcggtacc cttttgccgg accataaaag tcatgcagtt cccgaacgtg
    12601 cgcgcaaagc aagcgcttaa cctctgccaa aggcagcggg ggcagcaact ccccagtgtc
    12661 cagataatgc tggatttccc gaaagatcca gggtcttccc tgagctgcgc ggcctatcat
    12721 cagggcatcc gcccctgtat agtcgagcac agctctggct ttaagcgggt cagtaatgtc
    12781 gccattcgcg ataaccggaa tggaaacttt ctgcttaact gcccgaatac tgtcgtactc
    12841 agcttctcca ttgaacaaac aggcgcgtgt acggccatga atggtcagag cctgaatgcc
    12901 acagtcttca gccagttggg caatctcttc gcagttacgg tgttccggtg cccagccggt
    12961 gcgaatcttc agggtaacag gaacgtccac tgcattgacg acctcggtaa ggatcgattt
    13021 aacgacatcc gggtactgca agagggctga acctgcgagc ttgcgattca cttttttagc
    13081 cgggcaaccc atattgatat caataatctg ggcaccgctt tccacgttaa tacgtgctgc
    13141 atctgccatt tctttcggat cgctaccagc aatttgcacg gtgcgaatac cgggttcatc
    13201 aatgtgcacc atccgtaaac gagatttgtc gctttcccaa acctgtgggt tagaagacat
    13261 catctcggat actgtcaatc cggctcccat ctcgtagcac aacgtccgaa aaggtctgtc
    13321 tgtaatgcca gccatgggcg ctgcgatcag gcgatttctg agctgatatt gtccgatgcg
    13381 catgagttaa gaaatgacca tactgtgact gcaaggcggc gtatattacg cattttttgc
    13441 acgagatgaa aggccaaact ttgaccaatc ctctgagatg gatcaaagaa ttgcatttaa
    13501 aatgagcgtg gtgcgataat tactcataaa aatcatcata ttagaaaata gtgactaaaa
    13561 tttacactca aagaaatttg agtaagttct caatttttct ttatgaatga aaattttggc
    13621 acgcaaattt gcgtaaataa tcggcaaatt tacgtgcctt ttgtgagctt gctcgcactt
    13681 cgccccgcgt caccctacgg cgatgcgaag gttaattctt acgaccggta atacggcacc
    13741 actcttcttt ttccacgacc gggtccagtg cgaagctgtc ggcataagct tcacaaacgc
    13801 tctctgcctg gcttgccaga ataccggaaa ggcccagcaa accgcctgaa accggcagga
    13861 cgctgattaa cggtgccagt tcacgtaatg ggcctgcaag gatgttagcg accaccacgt
    13921 cggctttcat ttcttctggc tgatctttcg gtaagtagag ttccagacgg tcagaaacgc
    13981 cattacgttc ggcgttgtcg cggctggcct gaatcgcctg cggatcgata tcaataccaa
    14041 tggcttttgc tgcacccagt ttcagcgccg cgatcgccag aatgccggaa ccacagccaa
    14101 agtcgatgac tgttttaccg gttaaatcga ggctgtcgag ccattgcagg cacagagagg
    14161 tggttggatg ggtacccgta ccaaacgcca gccctggatc taacatcacg ttgacggcgt
    14221 tttcgtccgg cacatcacgc cagctagggc agatccacag tcgttcacca aagcgcatcg
    14281 ggtggaaatt atccatccat tcgcgctccc agtctttatc ttctagttgt tcgattttat
    14341 gcgcgaagcc tgcgccgagc agcggatggt tttccagaat cgccaccacg tcgttcatat
    14401 cggtttcagc gtcaaacaga ccaatcacat cggtgtcgcc ccacaggcgc gtttcgcccg
    14461 gcagcggctc aaatactggc gtatcgtggg tatcctgaaa agtgatagaa acggcacccg
    14521 cttccatcag cgcatcgcta agatcttccg cgttcgcgcc ggtggtgttc agtttcagtt
    14581 ggatccaagg catggcaaaa ctctttattt atcagtagtc aaaacggtag cttgcgggac
    14641 ggatgtaccg aaacggtttc cgaccaggaa agccagcaaa cttagtagta acgagggcac
    14701 gatagggtgg aagcccaggt actgaatatt cagcgtcgcg agtacggcat acagcacgcc
    14761 gccaacgatc atcgcactta gcgcgccttt ggcgttggcg cgttcccagt aaagacccag
    14821 caccagcggc cacaggaaaa cggcttccag cccaccgaag gccagcaaat tcagccagat
    14881 gatcatttct ggcggcttcc aggcggcaag cagcagcaac gcgccgagaa ctaacgtaat
    14941 taccgccgac atccgcttca gacgcgtctc gttttgcatt tgatccggac ggatattcag
    15001 atagagatct ttaatgatcg tagcggaact ttgcagcaac tgggcattga tcgtcgacat
    15061 gatcgcagcc ataggtgcag ccaggaagat tccggcagca aacggtggca gcacttttac
    15121 cattaacgtc gggatcacca gatccggtac ggtgagatcg gggatcactg cacgacctaa
    15181 cgctccggcc aggtgcatac cgaacatcag gattgcgact acaatcgtac cgatgatgat
    15241 cccccggtgt actgctttgc tgtctttata agagatacag cgcaccgcag tatgcggcag
    15301 gccaatcacg ccaaagcaca ccagtaccca gaacgacgtc ataaaggcag gcgacagaat
    15361 atcgtcagcg ccttgtggcg taaccagttg cggatcgatg gtttgcaagg tctgtactgc
    15421 gttgcttaag ccgccagcgg catgtaccac gccaataagc agcacaacgg tgccaatcag
    15481 catcacaagc ccttgcatgg tgtcgttcag cacgctggcg cgaaagccac caaaggcggt
    15541 atataacgca atgctgatac caaaaatcag cagcccggtt tcataaggaa tacccgccgc
    15601 ggtttccagc aggcgcgcac cgccgataaa ctgcacggtc atcgcaccaa cgaacgcaac
    15661 cagcaaactc aaactcgcca gccacaccag aagacgactc tggtagcggg caaacagcat
    15721 atcgttcagc gtcactgcat tgtagcggcg cgcaagaatc gcaaacttct tgccgagaat
    15781 accgagtgaa agccagactg caggaagctg aatcatcgcc agcaataccc agcccagccc
    15841 gtatttataa gcagctcctg gcccgccgat aaacgaactg gcactgatat aggtcgcggt
    15901 gagcgtcatc gccagcacaa taccgcccat agagcggctg ccgaggaaat actcattaag
    15961 gaaggtgccg gtgctccgtt tacgcatcgc ataaaccgag ataccgaaca ccaccaccag
    16021 ataggcgacc agcggtagaa ttacttcaag ctgcatcgtc atcctccagt gggatatcgc
    16081 gatagataaa tttcaccatc gcccagcaca gtccaataaa cagcagcggc gtcaggatgc
    16141 aggccatctc aaaccagcgc ggaaagccgg taaaaccggg ggcaacgcca ggtaagtaag
    16201 cggctactaa ccataccgcc agatacaaaa gggtcagccc cagcgcccag cgcgcctctt
    16261 tatgggcctg aacaaaacga gtgtccattt tttgtccctg atgggtgaag aaagcgagga
    16321 ttgtacctta tgggggttgt cgatccccag taataaaaaa ggccggaaaa tccggccttt
    16381 tgacgctttt gcagtcttat ttttcctgaa gaccgagttt tttctccaga tagtggatgt
    16441 tagtgccacc atgctggaag ttctcgtcat tcatgatgcg gatctgcaga tcaacgttgg
    16501 ttttgatacc gtcgatgatc agctcctgca gcgcattctt catgcgggca atcgccacgt
    16561 cacggttttc accgtagcaa atcagcttac cgatcattga gtcatagtac ggcggtacgg
    16621 tgtagcccgc gtagatatga gactcccaac gcactccaaa accgccaggt gcgtggaaac
    16681 gggtgatttt gcctgggctt ggcaggaagg tgttcggatc ttcggcgttg atacgacatt
    16741 ccaccgcatg gccgcgaacg tgaacttctt cttgcttgat cgacagcggt tgaccggcag
    16801 cgatacgcag ctgttctttg atcaggtcaa cgccggtgat catttctgta accgggtgtt
    16861 ctacctgaat acgggtgttc atttcgatga aatagaactc gccgttttcg aacaggaact
    16921 cgaaagtacc tgcaccgcga tagccgatat caacacacgc tttagcacaa cgttcgccga
    16981 tgtagcgacg cagttccggg gtaatgcccg gtgctggtgc ttcttcgacc actttctggt
    17041 ggcggcgctg catggagcag tcacgttccg ccagatagat agcgttgccc tgaccgtcag
    17101 ccagtacctg aatctcgacg tggcgaggat tttccaggta tttctccatg taaaccatat
    17161 cgttgctgaa agcagctttc gcttccgcac gggtcatgga gatggattgt gccagttcag
    17221 cgtcgccgcg cactacgcgc ataccgcgac caccgccgcc gccggaggct ttgataatca
    17281 ccggataacc aatgcgttta gcaatggcac ggtttttatc catatcgtcg cccagcgggc
    17341 cgtcagaacc cggtacgcaa ggaacgcccg cttttttcat cgccgcgatt gcggatactt
    17401 tgtcgcccat caggcgaatg gtttctgctt tcgggccgat gaagataaag ccggagcgtt
    17461 caacctgctc ggcaaagttg gcgttctcgg agaggaagcc gtaacccgga tggattgcta
    17521 ctgcgccggt gatttcagcg gcgctgatga ttgccgggat gttcagataa ctttttactg
    17581 acggagcagg gccgatacag accgtttcat ctgccagtaa tacgtgtttt agatcgcgat
    17641 ccgcgctgga gtgcacagcg acagtcttga tgcccagttc tttacaggca cgaagaatac
    17701 gcaatgcaat ctcgccgcgg ttggcaataa caattttatc cagcatgttc gcctcgttac
    17761 tcgatgacga ccagcggctc gtcaaattct accggttgtc cactttcgac cagaattgct
    17821 ttcacggtac cggatttgtc cgcttcgatc tggttcatca ttttcatggc ttcaacgatg
    17881 cacagggtat cgcccacgtt gactttctga cccacttcga tgaacgcttt tgcgtccggg
    17941 cttggggtgc ggtagaaagt accaaccatc ggggaacgta cgatgtgacc actgatttcc
    18001 gctgctgctg gcgcttccat ggaaggaacg gtcgccggag cggctgcgtt agattgagct
    18061 ggctgctgca tcattggtgc agcgtaagct tgttgcatca cagggaaact tgcggcagga
    18121 gctgcacggc taatgcgtac tgactcttcg ccttcagaaa tttccagttc ggagatgcct
    18181 gattcttcaa ccagctcgat cagtttttta atcttacgaa tatccatgag tgggttccgt
    18241 actctttgtt tagtgtgatt gtgacaggcg tttcaccgcc gtctgtaaag cgtatgcccc
    18301 gacagctgta tgcatagcga taaattccag caggccggga cgccggtcta ttttgcccca
    18361 gagtgtcaat gttagacttg acggacattg tgcagcgatc tgctgctatc ctccggcaaa
    18421 aaacaaaata taccttcgtt gcgccaatgt caccttttcc gcagcgcaaa aactgcgtca
    18481 gggaggacga ggtcgcacat tataacgatt tcgtagcaat tggcagctaa atactggtct
    18541 tatcagggaa gataatcaac agctaacatg taaataacct tcaacaccgt gtaatttgca
    18601 acaagccgca caattcacga aattagcgcc atcatcgacg gaacttctta taacgtaagg
    18661 tgaaaagctg taaagccagc cctgcgaaga tgaggggctg caatgagata atcttcacag
    18721 accacaaata atgtagggat gccaggatcg cgacaagata gacgacatta cgcagcggtt
    18781 gctggggatt acccaatttt cactgcattg tctggataga tgttaatgct aaagcacgca
    18841 aaattaaccg attgacaatg ctcagcatta aataggttcg agtgattaat ttgtagtcaa
    18901 ataaaatcag attattcacc ccatggtacc agaaatcagt aacaggttaa gcgtagtgtc
    18961 gcccaggcaa agcaccataa acctaacagg cgacggaggc atatcaataa tggctgttta
    19021 gaggagtatc aatgcgcatc aatttgtggt cagtcgaaat catgactttc cagtttggat
    19081 gatgttgtac aacttcctga ccaaggtgtt cgacgaaagc tgtccggcta accgcttttc
    19141 ccgttaaccg attgaataat gatgtcaaaa tgagttccct ctcttattat tccttgctaa
    19201 atggttagtt aaccttcacc agcgtgcgac cctggatctg gttattaatg atggcctcgg
    19261 caaagttcgg tgcctctgac agagatatct cttttgccgc ctgggtatag aatgattccg
    19321 gtaaatcggc gaccagtcgc tgccaggctt gtgcgcggcg ttctggtggc gtcattactg
    19381 aatccacccc ttgcaaacgg acattacgca gaataaatgg catgaccgtg gttggcagag
    19441 taaaaccacc cgccagacca caggccgcca cgcagccgcc gtaattcatt tgcgccagca
    19501 cttttgccag cactttgtcg ccaacagtgt caattgcccc agcccagact tgtttttcca
    19561 gaggacggga ttcggcaaac tcatcacgag ggagaatacg actggcacct aaacttttca
    19621 ggtattcatg ggtactttcg cgaccggaaa cggcaacgac ctgataaccc aacttatgca
    19681 gcagcgccac ggcggtactg ccgacgccac cactggcacc cgtcacgaca atctccccgt
    19741 cctgcgggcg aacaccggca tcttccagcg ccatcacaca cagcatggcg gtaaaaccgg
    19801 cagtaccgat aatcattgct ttacgcgcgt ccagcccttg cggcatggca accagccagt
    19861 cacctttcac tcgcgcctgc tccgccagcc caccccagtg gttttcacca acgccccagc
    19921 cagtgagtaa cacctcctga ccggcatgaa aacgcggatc ttcgctggtg cgtacagttc
    19981 cggcaaaatc gatcccagga atcatcggaa aattacggat gatttttccc ttaccggtaa
    20041 tcgccagcgc atctttatag ttcaggctcg accagtgaac atcaaccgtg acatcgccct
    20101 ccggcaggcg actttcgtca agtgtctgta ccgatgcgag agttttgccg tcctgctgtt
    20161 ctaaaagtaa cgcctgcata agtggtcctc atgtgcatga tggattggaa aataatttct
    20221 gaagactata ctcgctaatg gaaaagcaaa gccgatgaag cgcaataaat tgcgagataa
    20281 atctgatttg ctagtatgcc cgcttcctca ctatcggagt taacacaagg atgagattaa
    20341 cgacgaaatt ttcggccttt gttacgctgc tcaccgggtt aacaattttt gtgactttgc
    20401 tgggctgttc gctaagtttc tacaacgcaa ttcagtataa gtttagtcat cgcgttcagg
    20461 cggtggcgac ggcgattgat acccaccttg tgtcgaatga cttcagcaca ttaaggccac
    20521 aaattaccga attaatgatg tcggcagata tcgttcgtgt agacctgctc catggtgata
    20581 aacaggttta taccctggcc agaaatggta gttatcgtcc ggttggctcc agcgatctgt
    20641 ttcgcgaact gagtgttccg ttgataaagc atccggggat gtcgctgcgt ctggtttatc
    20701 aggatccgat gggcaactat ttccattctt taatgactac cgcgccgctc acgggggcga
    20761 ttggctttat cattgttatg ctcttcctgg cggtacgctg gttacaacgg caacttgccg
    20821 ggcaagaatt gctggaaacc cgggctactc gtatcttaaa cggtgagcgt ggctctaatg
    20881 tgttgggaac catctatgaa tggccgccca gaaccagcag tgcgctagat acgctgcttc
    20941 gtgaaattca gaacgcacgc gaacaacaca gccgtcttga tacgctgatc cgctcttatg
    21001 ccgcccagga cgtgaaaacc ggcctcaata accgactctt tttcgataat cagttagcaa
    21061 cgttactgga agatcaggag aaagtaggta cccacgggat cgtgatgatg attcgtctgc
    21121 cggatttcaa tatgttgagc gatacctggg ggcacagcca ggttgaagaa cagttcttca
    21181 ctctgacgaa tctgctgtcg acatttatga tgcgctaccc tggcgcactg ctggcgcgtt
    21241 accaccgcag tgattttgct gcgctgttac cgcaccggac gttaaaagag gcagagagca
    21301 tcgccggtca gttaatcaaa gccgtcgata ccttgccgaa caataaaatg ctcgatcgcg
    21361 acgatatgat ccacattggt atctgcgcct ggcgtagtgg tcaggatacc gagcaggtaa
    21421 tggaacatgc agagtctgcc acgcgtaatg cgggattgca gggcggcaat agctgggcta
    21481 tttacgatga ctcgttgcct gaaaaaggac gcggtaatgt tcgctggcgt acgcttatcg
    21541 agcaaatgct cagtcgcggc ggcccgcgcc tttatcaaaa accggcggtt actcgcgaag
    21601 gtcaggttca tcatcgcgaa ctcatgtgcc gcatcttcga tggtaatgaa gaggttagct
    21661 cggcggagta tatgccgatg gtcttgcagt ttggcttatc ggaagagtat gaccgtctgc
    21721 aaatcagccg tcttattcca ctattgcgtt actggccaga ggaaaatctg gcgattcagg
    21781 ttaccgttga gtcgctgatt cgcccgcgtt ttcagcgttg gctgcgcgat acgttaatgc
    21841 aatgtgaaaa atcacaacga aaacgcataa ttattgaact tgcagaggcc gatgtaggtc
    21901 aacatatcag tcgtttacaa cctgttattc gtttagtgaa tgctttaggg gtacgggtag
    21961 ccgtcaacca ggctggtttg acgctggtaa gcaccagttg gatcaaagaa cttaatgttg
    22021 agttactcaa gctccatccg gggctggtca gaaacattga gaagcgaacg gagaaccagc
    22081 tgctggttca aagcctggtg gaagcctgct ccgggaccag cacccaagtt tacgccaccg
    22141 gtgtgcgttc gcgaagcgag tggcagaccc tgattcagcg cggtgttaca ggcgggcaag
    22201 gggatttttt cgcgtcctca cagccacttg atactaacgt gaaaaaatat tcacaaagat
    22261 actcggttta acctgccgtt taatctgttt tcacgtagaa taacgcgcgc tgcgtctcat
    22321 gggagtgtgc ttgtctgctc gccagattgt tgcagcacat atgcagatga atgaccttac
    22381 gcggttgcaa acaggcgagg aatgctgctg atgcattaag cctttctgga ctcaggcaga
    22441 gatttgtaac aaaggaaacg aactgcacta attttcaccg tagcagatga tttttgcgcc
    22501 ttgtcgctgc tgcgtgtggt tggtaaagta agcggatttt cttttccgcc ccagctttca
    22561 ggattatccc ttagtatgtt gaaaaaattt cgtggcatgt tttccaatga cttgtccatt
    22621 gacctgggta ctgcgaatac cctcatttat gtaaaaggac aaggcatcgt attgaatgag
    22681 ccttccgtgg tggccattcg tcaggatcgt gccggttcac cgaaaagcgt agctgcagta
    22741 ggtcatgacg cgaagcagat gctgggccgt acgccgggca atattgctgc cattcgccca
    22801 atgaaagacg gcgttatcgc cgacttcttc gtgactgaaa aaatgctcca gcacttcatc
    22861 aaacaagtgc acagcaacag ctttatgcgt ccaagcccgc gcgttctggt ttgtgtaccg
    22921 gttggcgcga cccaggttga acgccgcgca attcgtgaat ccgcgcaggg cgctggtgcc
    22981 cgtgaagtct tcctgattga agaaccgatg gctgccgcaa ttggtgctgg cctgccggtt
    23041 tctgaagcga ccggttctat ggtggttgat atcggtggtg gtaccactga agttgctgtt
    23101 atctccttga acggcgtggt ttactcctct tccgtgcgca ttggtggtga tcgtttcgac
    23161 gaagctatca tcaactatgt gcgtcgtaat tacggttctc tgatcggtga agccaccgca
    23221 gaacgtatca agcacgaaat cggttcggct tacccgggcg atgaagtccg tgaaatcgaa
    23281 gttcgtggcc gtaacctggc agaaggtgtt ccacgcggtt ttaccctgaa ctccaacgaa
    23341 atcctcgaag cactgcagga accgctgacc ggtattgtga gcgcggtaat ggttgcactg
    23401 gaacagtgcc cgccggaact ggcttccgac atctccgagc gcggcatggt gctcaccggt
    23461 ggtggcgcac tgctgcgtaa ccttgaccgt ttgttaatgg aagaaaccgg cattccagtc
    23521 gttgttgctg aagacccgct gacctgtgtg gcgcgcggtg gcggcaaagc gctggaaatg
    23581 atcgacatgc acggcggcga cctgttcagc gaagagtaat cggatgcagg caggggaaat
    23641 gtctgtttac cctgcctggt ctgatacgag aatacgcata acttatgaag ccaattttta
    23701 gccgtggccc gtcgctacag attcgcctta ttctggcggt gctggtggcg ctcggcatta
    23761 ttattgccga cagccgcctg gggacgttca gtcaaatccg tacttatatg gataccgccg
    23821 tcagtccttt ctactttgtt tccaatgctc ctcgtgaatt gctggatggc gtatcgcaga
    23881 cgctggcctc gcgtgaccaa ctagaacttg aaaaccgggc gttacgtcag gaactgttgc
    23941 tgaaaaacag tgaactgctg atgcttggac aatacaaaca ggagaacgcg cgtctgcgcg
    24001 agctgctggg ttccccgctg cgtcaggatg agcagaaaat ggtgactcag gttatctcca
    24061 cggttaacga tccttatagc gatcaagttg ttatcgataa aggtagcgtt aatggcgttt
    24121 atgaaggcca gccggtcatc agcgacaaag gtgttgttgg tcaggtggtg gccgtcgcta
    24181 aactgaccag tcgcgtgctg ctgatttgtg atgcgaccca cgcgctgcca atccaggtgc
    24241 tgcgcaacga tatccgcgta attgcagccg gtaacggttg tacggatgat ttgcagcttg
    24301 agcatctgcc agcgaatacg gatattcgtg ttggtgatgt gctggtgacc tccggtctgg
    24361 gcggtcgttt cccggaaggc tatccggtcg cggttgtctc ttccgtaaaa ctcgataccc
    24421 agcgcgctta tactgtgatt caggcgcgtc cgactgcagg actgcaacgt ttgcgttatc
    24481 tgctgctgct gtggggcgcg gatcgtaacg gcgctaaccc gatgacgccg gaagaggtgc
    24541 atcgtgttgc taatgaacgt ctgatgcaga tgatgccgca ggtattgcct tcgccagacg
    24601 cgatggggcc aaagttacct gaaccggcaa cggggatcgc tcagccgact ccgcagcaac
    24661 cgacgacagg aaatgcagct actgcgcctg ctgcgcccac acagcctgct gctaatcgct
    24721 ctccacaaag ggctacgccg ccgcaaagtg gtgctcaacc gcctgcgcgt gcgccgggag
    24781 ggcaatagtg gcgagctatc gtagccaggg acgttgggta atctggctct ctttcctcat
    24841 tgcgctgttg ctgcaaatca tgccctggcc ggataacctg attgttttcc ggccaaactg
    24901 ggtgttactc atcttgttgt attggatcct ggccttgcct catcgcgtaa atgtgggcac
    24961 aggttttgtg atgggtgcca tactggatct gatcagcggc tcgacgcttg gcgtacgcgt
    25021 attggcgatg agcatcattg cttacctggt ggcgctgaaa taccagcttt tccgcaacct
    25081 cgcattatgg cagcaggcgc tggtcgtcat gttgctttcg ctggtggtgg atattattgt
    25141 tttctgggca gagtttttag tgattaacgt ctctttcaga ccggaagtgt tctggagtag
    25201 tgtagtcaat ggggtgctct ggccgtggat tttcttgctg atgcgtaaag tccgtcagca
    25261 gtttgcagtg caataaaggt ttctatgact tctctgtatt tagcttccgg ttctccgcgt
    25321 cgtcaggagt tacttgcgca acttggcgtg acctttgaac gtattgttac aggcattgag
    25381 gagcagcgtc agccgcagga gagcgcgcag cagtatgttg tgcgtctggc gcgcgagaaa
    25441 gcacaggcgg gtgtcgcgca aacggcgcag gatctcccgg tgctgggtgc ggatactatc
    25501 gttatcctga atggagaagt gctggagaaa ccgcgcgacg cagagcatgc ggcgcagatg
    25561 ttgcgcaaat tatcgggtca gacccatcag gtgatgacgg cagtggcgtt ggccgacagc
    25621 cagcacattc tcgattgcct ggtggtcacc gatgtgactt tcagaacgtt aacagacgaa
    25681 gacatcgcgg gctatgtcgc cagcggtgaa ccgttagata aagcaggtgc atacggtatt
    25741 caggggctgg gtggctgttt tgtcaggaag ataaatggca gctatcacgc cgtagtcggc
    25801 ttaccgctgg ttgaaacgta tgaattatta agtaatttta acgcactgcg tgagaaaagg
    25861 gataaacatg acggctgaat tgttagtaaa cgtaacgcct tcggaaacgc gagtggcgta
    25921 tattgatggc ggtattctgc aggaaattca tattgaacgt gaggcgcgac gcggaatagt
    25981 aggcaatatc tacaagggtc gtgtaagtcg tgtacttccg ggtatgcagg cggcttttgt
    26041 agatattggg ctggataaag ccgcgtttct tcatgcatcc gatatcatgc cgcacaccga
    26101 atgtgtggcg ggtgaagaac aaaagcaatt cacggtgcgc gacatctcgg aactggttcg
    26161 tcaggggcaa gatctgatgg tgcaggtggt gaaagatccg cttggcacta aaggtgcgcg
    26221 cctgaccacc gatatcacgc tgccttctcg ctatctggtg tttatgccag gggcttctca
    26281 cgttggggtt tcccaacgta ttgaaagcga atcagaacgt gaacgcctga aaaaagtggt
    26341 cgcagagtat tgcgacgagc agggcgggtt tatcatccgt accgcagcgg aaggggttgg
    26401 cgaggctgaa ctggcctccg atgccgctta tctgaaacgc gtctggacca aagttatgga
    26461 gcgtaaaaaa cgcccgcaga cccgttatca gctgtacggc gaactggcgc tggcgcagcg
    26521 tgttctgcgt gatttcgccg acgccgaact ggaccgcatt cgcgttgact cacgcctgac
    26581 ttacgaagca ttgctggagt ttacctcgga gtacattccc gagatgacca gcaagctgga
    26641 gcattacacc ggacgccagc cgattttcga tctctttgat gtcgaaaacg aaatccagcg
    26701 agcgctggaa cgcaaagtag aactgaaatc cggtggttat ctgattatcg accagaccga
    26761 agcgatgacc accgtggaca tcaataccgg agcgtttgtc ggtcatcgca atctggacga
    26821 caccattttc aataccaata ttgaagcgac gcaggctatc gctcgccagt tacggttgcg
    26881 taatctgggt gggattatca ttattgattt catcgatatg aataatgaag atcaccgccg
    26941 ccgcgtgctg cactcgctgg agcaggcgtt gagcaaagac cgggtgaaaa ccagcgttaa
    27001 tggtttttcg gcgctggggc tggtggagat gacgcgtaaa cgcacccgcg aaagcattga
    27061 gcacgtactg tgtaacgaat gcccaacctg ccacggtcgc ggaacggtga aaaccgtgga
    27121 aacggtatgc tatgaaatca tgcgcgagat tgttcgtgtc caccatgctt acgactccga
    27181 ccgtttcctg gtctatgctt ctccggcagt agctgaagcc ttgaaaggcg aagagtcaca
    27241 ctcgctggcg gaagtggaaa ttttcgttgg caaacaggtt aaagtacaaa ttgaaccgct
    27301 ctataaccag gagcagtttg acgtcgtaat gatgtaaaca gatgctgggc cgccatccgg
    27361 caaagggttt ttgagtcaca tttttagcag acaaggagtg acgggtgagg cgattgccgg
    27421 ggattttact gcttactgga gccgcgctcg ttgtgatcgc tgctctgctg gttagcggcc
    27481 tgcgtattgc tttaccgcat cttgacgcct ggcgtccgga aatcctcaac aaaatagaat
    27541 ccgcgactgg catgccggta gaagccagtc agctctcagc cagctggcag aattttggcc
    27601 cgacgcttga agcacacgac atccgtgcag aactaaaaga tggcggcgaa ttttcggtta
    27661 aacgcgttac tctggcgctg gatgtctggc agagcctgtt acatatgcgc tggcagtttc
    27721 gcgacctcac tttctggcag ctgcgctttc gcaccaacac tcctatcacc agcggtggta
    27781 gcgatgacag tctggaagcc agtcacatca gcgatctgtt tcttcgtcaa tttgaccatt
    27841 tcgatctccg cgacagtgaa gtcagttttc tgacgccatc cggtcagcgc gccgagctgg
    27901 cgatcccaca actcacctgg ctgaacgatc cacgtcgaca ccgtgcggaa ggcctggtaa
    27961 gcctctccag ccttaccgga cagcacggcg tgatgcaggt gcgtatggat ttgcgcgatg
    28021 atgaggggtt gttaagcaat ggtcgcgtct ggctacaggc ggatgacatc gacctgaagc
    28081 cgtggctcgg taaatggatg caggacaata ttgctctgga aacggcacag ttctcccttg
    28141 aaggctggat gacgatcgac aaaggcgatg taaccggcgg tgacgtctgg ctgaaacagg
    28201 gcggtgccag ctggttgggc gagaagcaaa cgcatacgct gtcggtggat aatctgaccg
    28261 cgcatattac gcgtgaaaat ccgggctggc agttctctat tcccgataca cggatcacga
    28321 tggacggcaa accctggccg agcggagcat tgacgctggc ctggataccg gaacaggacg
    28381 ttggcggcaa agacaataaa cgcagtgacg aactccggat tcgcgccagt aatctggagc
    28441 tggcaggcct ggagggcata cgcccgctgg ccgcgaaact ttcacctgca ctgggtgatg
    28501 tttggcgctc cacacaaccg agcggcaaga ttaacactct ggcgctggat atcccgcttc
    28561 aggcggcaga caatacccgt tttcaggcat cgtggagcga tctggcctgg aagcaatgga
    28621 aattattacc gggtgcggaa cacttctccg ggacgctttc cggcagcgtt gaaaatggtt
    28681 tgcttaccgc gtcgatgaag caggcaaaga tgccttacga aacggtattc cgtgcgccac
    28741 tggaaatcgc cgacggccag gcaactataa gctggctgaa caatgacaaa ggtttccagc
    28801 tggatgggcg taatattgac gttaaagcca aagccgtcca tgcgcgcggc ggttttcgtt
    28861 acctgcaacc tgctaacgat gaaccctggc tgggtattct ggctggcatc agtaccgatg
    28921 atggttcaca agcctggcgc tatttcccgg aaaacttgat gggtaaagac ctggttgatt
    28981 acttaagtgg cgcgattcag ggcggtgaag cggataacgc gacgctggtt tatggtggca
    29041 atccgcaact cttcccctat aaacacaacg aaggtcagtt tgaagtgctg gtgccgctgc
    29101 gcaacgcgaa gtttgccttc cagccggact ggcctgcatt aactaacctt gatattgaac
    29161 tggactttat taacgacggt ttatggatga aaaccgatgg cgttaatctg ggcggcgtgc
    29221 gcgcgagtaa tctcaccgca gtgatccctg actactcaaa agaaaaactg ctgattgacg
    29281 ctgacattaa aggtccgggt aaagccgtag gcccttactt tgatgagaca ccgctgaaag
    29341 attctctggg tgcgaccctg caagaactcc agctcgacgg cgatgtgaat gctcgcttac
    29401 atcttgatat cccgctgaac ggcgagctgg taaccgcgaa aggtgaagtg acgctgcgta
    29461 ataacagtct gtttatcaaa ctactcgaca gcaccctgaa aaatttgagc ggtaaattca
    29521 gctttatcaa tggcgatctg caaagtgaac cactgacagc aagctggttt aatcagccgt
    29581 tgaacgtgga tttttccacc aaagaagggg caaaagccta ccaggttgcg gtgaatctca
    29641 acggtaactg gcaaccggcg aaaaccggcg ttctgcctga agcggtgaac gaagcattga
    29701 gtggcagcgt ggcgtgggat ggtaaagtgg gcattgagct gccttatcat gctggcgcga
    29761 cctataacgt agagctgaac ggcgatctga agaatgtgag cagtcactta ccttcaccgt
    29821 tagccaaacc tgcgggtgaa ccactagcgg taaacgttaa ggttgatggc aatctcaaca
    29881 gctttgaatt aaccggacag gctggtgcgg ataaccattt caatagccgc tggttgcttg
    29941 gtcaaaagct gacgctcgat cgtgctattt gggcggcaga cagtaaaacg ctcccgccgt
    30001 tgccggaaca aagtggcgtt gaactcaata tgccgccgat gaatggtgcc gagtggctgg
    30061 ccctgttcca gaaaggcgct gcggagagtg tcggtggtgc agcgagtttc ccacaacaca
    30121 taacgttacg tacgcctatg ttgtcactgg gaaatcagca atggaataac ctgagtattg
    30181 tttcgcaacc gacggcaaat ggcaccctgg ttgaggcgca ggggcgtgaa atcaatgcca
    30241 cgctggcgat gcgtaataac gcgccgtggc tggcgaatat caaatatctt tattacaacc
    30301 cgagcatggc gaaaactcgt ggtgattcaa caccgtcatc acctttcccg acaacggagc
    30361 gcattaactt ccgtggctgg ccggacgcac aaatacgatg cacagagtgc tggttctggg
    30421 ggcaaaaatt cggtcgtatt gacagtgatc tcaccatttc tggcgatacg ttaacgctga
    30481 ccaatggact gattgatact ggtttctcgc ggcttactgc cgatggtgaa tgggttaata
    30541 atccggggaa tgaacgtacc tcgctgaaag gaaaactgcg cgggcagaaa attgatgccg
    30601 ccgcagaatt ttttggtgtc acgacgccca tacgccagtc gtcatttaat gtggattacg
    30661 atttacactg gcgcaaagca ccctggcaac cagatgaggc gacgttgaat ggcatcattc
    30721 atactcaact gggtaaaggc gaaattaccg aaatcaatac cggacatgcc gggcaattgc
    30781 tgcgcttatt gagcgttgat gccctgatgc gtaagctgcg ttttgatttc agagacactt
    30841 ttggcgaagg gttctatttt gactccattc gcagcaccgc gtggattaaa gacggggtta
    30901 tgcacaccga cgacacgctg gtggatggcc tggaggcgga tatcgccatg aaagggtcgg
    30961 taaatctggt acgtcgcgac ctgaatatgg aagcggttgt cgcaccagag atttctgcga
    31021 cggtgggcgt ggctgcggct tttgcggtta accccattgt tggcgcggca gtatttgcag
    31081 ccagtaaagt gctggggccg ctgtggagca aagtctccat tttgcgctat cacatttcgg
    31141 gtccgctgga cgatccgcaa atcaacgaag tgttgcgcca accgcgtaaa gaaaaagcgc
    31201 aatgatttga cgagggcgcg taattgcccc aatctcatat gataatcgtt gccaaaggcc
    31261 aacgagccgg aacataactg taggccggat agggcgttca cgccgcatcc ggcagccgta
    31321 aaaaatcctc tactgcagta actaacgagt agcaaaaacg atgagtctga acctggtaag
    31381 tgaacaattg ctagcggcga acggcctgaa acatcaggac ttgttcgcga tcctcggtca
    31441 actggccgaa cgtcgccttg attatggcga tctctatttt cagtcgagct atcacgaatc
    31501 ctgggtttta gaagaccgca ttattaaaga tggttcttac aacatcgatc agggcgttgg
    31561 tgtgcgtgca atcagtggtg aaaaaaccgg atttgcttac gctgaccaaa tcagcctgct
    31621 ggcgctggaa cagagtgcgc aagcggcgcg caccatcgtc cgtgatagtg gtgatggcaa
    31681 agtacagacg ctgggcgcgg tagagcatag cccgttgtat acctcggtag atccgctgca
    31741 aagcatgagc cgtgaagaga agctggatat cctgcgtcgc gtcgataagg ttgcccgcga
    31801 agcggacaag cgcgtacaag aagtgactgc cagcctcagc ggcgtttatg aattaattct
    31861 ggttgcggcc accgacggca cgctggcggc ggatgttcgt ccgctggtgc gtctttccgt
    31921 gagcgttctg gtcgaagaag atggcaaacg cgaacgcggt gccagtggcg gcggcggtcg
    31981 ttttggttat gagttcttcc ttgccgatct tgacggcgaa gtccgtgcgg atgcatgggc
    32041 aaaagaagca gtgcgtatgg cgctggtcaa tctttctgcc gttgctgcac cagcgggcac
    32101 catgccggta gtacttggcg caggttggcc gggcgtgctg ttgcatgaag cggtaggtca
    32161 cggtctggaa ggcgacttca accgccgcgg cacttcagta tttagtgggc aggtcgggga
    32221 gctggtggct tcagaactgt gtaccgtggt tgatgacggc acgatggtcg atcgccgtgg
    32281 ttcggtggcg attgatgacg aaggtacgcc aggccagtac aacgtgctga ttgagaacgg
    32341 cattctgaaa ggctacatgc aggataaact caacgcgcgt ttgatgggga tgacgccgac
    32401 tggcaacggt cgccgtgaat cctacgccca tctgcccatg ccgcgtatga ccaacaccta
    32461 tatgctgccg ggtaaatcga ccccgcagga aattattgaa tccgttgagt acggtatcta
    32521 tgcaccgaac tttggtggcg gtcaggtgga tattacatcc ggcaaattcg ttttctccac
    32581 ttcagaagcg tatctgattg aaaacggtaa agtaacgaag ccggtgaaag gcgcgacgtt
    32641 gattggttcc ggtatcgaaa ccatgcagca gatttcgatg gttggcaacg acctgaaact
    32701 ggataacggc gtgggtgtct gcggtaaaga agggcaaagt ttgccggttg gcgtgggcca
    32761 gccaacgctg aaagttgata acctgactgt tggcggtact gcgtaataat ttcattattt
    32821 tcagaaggat aattaatctt tctacgtgca cgaacggtcc cctcgctcct ctggggttag
    32881 ggtgagggga acccgttggc acaggtttgt agaacgtaac agtacaatat gaattacttc
    32941 tctttcccgc gcccgtgcat ctcctgaaac aatttaccga cctcaacaaa ataatccgtc
    33001 agcgagttga tcacgacctg taccttcagc ggcagcttat ctttttcggt atataacgca
    33061 taaaccgggc gtggatctga ctgataacgc ggcagcagga tctccagctc accgcgattg
    33121 atctcgttga tcacccacat cagcggcacg taggcgatcc cggcacccgc cgttagccag
    33181 cgcaccagcg tcatcggatc attagtcaca aatctccctt gcgggatcag gcgagtcgag
    33241 atcccttccg gtgcaatcag ttcaaattca ttgtcgggcc gcacgctgta ttcaagccat
    33301 gaatggctac ttaaatcggc gggtttttcc ggtatgccgt attgtgtgag atagcttttc
    33361 gcggcgcaca ccaccattgg catcgcgcca agacggcggg aaaacaggct ggaatcctgc
    33421 aacgcgccga cgcggatcac cacatccaga ccgtcggcaa tcaggtcggg ggctggaatt
    33481 ccggtaacca gattgacgct caaacctggg tattctttca gcattttggc tgtcagcccg
    33541 gcgagaacat tttgtgccat agttgaagaa cagccaatgc gtagcgtccc gatgggggtg
    33601 ttattgaagg catacagttg ctcatgaaca tcctgcactt catgaagcat acgacggcag
    33661 ccctggtagt aaattctacc ggcttcggtc aggccaatgc tgcgtgtgct acggtttaac
    33721 agctttacct gcaactcatc ttccagtttt gacaccgtct gactgatgga cgaaacgctc
    33781 atctgtagct gtctggcggc ggcggtaaaa gagccaaatt caactacttt ggcaaacacc
    33841 gacatgcgtt ttagtcgttc cattattcac tctgacttaa aagtgattta gatcacataa
    33901 tatagataac agcataacag ttacgctaat atattaaata tcaatctaca gcaatgttgc
    33961 tctcgcccgg ctttccatgc ccttctctgc ggcgacagat gctgaaaata ataacgcctg
    34021 ctctctctat acaaccaagg tcaacatgag tctgtttccc gttatcgtgg tgtttgggct
    34081 gtccttccca ccgatatttt ttgaattgct tttatcactg gcgattttct ggctggtgcg
    34141 ccgggtactt gtgccaacag gtatctacga ctttgtctgg catccggcgt tgttcaacac
    34201 cgcgctctat tgctgcttgt tttatttgat atcgcgactg ttcgtttgag gttgaagtga
    34261 aaacactaat aagaaaattc tcccgtacgg ccatcacggt cgtattagtc attctggcct
    34321 tcatcgcaat ttttaatgcc tgggtctatt acaccgaatc cccctggacg cgtgacgcgc
    34381 gctttagcgc tgacgtcgtt gcgatcgcgc cggacgtttc tggactcatt acccaggtga
    34441 atgttcatga taaccagctg gtgaaaaaag gacagatact gttcaccatc gaccagccgc
    34501 gctatcaaaa ggcgcttgag gaagcgcaag ccgatgttgc ttattatcag gtactggcac
    34561 aggagaaacg ccaggaggcc ggacgtcgta accgtctcgg tgtgcaggcg atgtctcgcg
    34621 aagagatcga ccaggctaac aacgtactac aaacggttct gcatcagtta gcgaaagcgc
    34681 aggcgacccg cgatctggca aaactggatc ttgaacgcac ggtgatccgc gcgccagcag
    34741 atggctgggt gaccaacctc aacgtctata ccggtgagtt tattactcga ggatcaacgg
    34801 cggttgcgct ggtgaaacag aactccttct atgtactggc ctatatggaa gaaactaagc
    34861 tggaaggggt gcgtccgggg tatcgtgcag agatcacgcc gcttggcagt aacaaagtgc
    34921 tgaaagggac tgttgatagt gttgccgctg gggtcaccaa cgccagcagc acgcgtgacg
    34981 acaaagggat ggcgaccatc gactctaacc ttgaatgggt gcgtttggcg caacgtgttc
    35041 cggttcgtat tcgtctcgac aaccagcaag agaacatctg gcctgcgggc accactgcta
    35101 cagtggtggt cactggcaaa caagatcgcg acgaaagcca ggattcgttc ttccgtaaaa
    35161 tggcccatcg cctgcgtgag tttggttaat cacgatgggt attttctcca ttgctaacca
    35221 acatattcgc tttgcggtaa aactggcgac cgccattgta ctggcgctgt ttgttggctt
    35281 tcacttccag ctggaaacgc cacgctgggc ggtactgaca gcggcgattg ttgccgccgg
    35341 tccggccttt gctgcgggag gtgaaccgta ttctggcgct attcgctatc gtggcttttt
    35401 gcgcatcatc ggcacattta ttggctgtat tgccggactg gtgatcatca ttgcgatgat
    35461 ccgcgcacca ttattgatga ttctggtgtg ctgtatctgg gccggttttt gtacctggat
    35521 atcctcgctg gtacgaatag aaaactcgta cgcgtggggg ctggccggtt ataccgcgct
    35581 gatcattgtg atcaccattc agccggaacc attgcttacg ccgcagtttg ccgtcgaacg
    35641 ttgtagcgag atcgttatcg gtattgtgtg tgcgattatg gcggatttgc tcttttctcc
    35701 gcgatcgatc aaacaagaag tggatcgaga gctggaaagt ttgctggtcg cgcaatatca
    35761 attaatgcaa ctctgtatca agcatggcga tggtgaagtt gtcgataaag cctggggcga
    35821 cctggtgcga cgcaccacgg cgctacaagg catgcgcagc aacctgaata tggaatcttc
    35881 ccgctgggcg cgggccaatc gacgtttaaa agcgatcaat acgctatcgc tgacgctgat
    35941 tacccaatcc tgcgaaactt atcttattca gaatacgcgc ccggaattga tcactgatac
    36001 tttccgcgaa ttttttgaca cgccggtaga aaccgcgcag gacgtccaca agcagctcaa
    36061 acgcctgcgg agagttatcg cctggaccgg ggaacgggaa acgcctgtca ccatttatag
    36121 ctgggtcgcg gcggcaacgc gttatcagct tctcaagcgc ggcgttatca gtaacacaaa
    36181 aatcaacgcc accgaagaag agatcctgca aggcgaaccg gaagtcaaag tagagtcagc
    36241 cgaacgtcat catgcgatgg ttaacttctg gcgaaccaca ctttcctgca ttctgggcac
    36301 gcttttctgg ctgtggacgg gctggacttc cggcagtggt gcaatggtga tgattgcggt
    36361 agtgacgtca ctggcaatgc gtttgccgaa tccacgcatg gtggcgatcg actttatcta
    36421 cgggacgctg gccgcgctgc cgttagggct gctctacttt ttggtgatta tccctaatac
    36481 ccaacagagc atgttgctgc tgtgtattag cctggcagtg ctgggatttt tcctcggtat
    36541 agaagtacag aaacggcgac tgggctcgat gggggcgctg gccagcacca taaatattat
    36601 cgtgctggat aacccgatga ctttccattt cagtcagttt ctcgacagcg cattagggca
    36661 aatcgtcggc tgtgtgctcg cgttcaccgt tattttgctt gtgcgggata aatcgcgcga
    36721 caggaccgga cgtgtactgc ttaatcagtt tgtttctgcc gctgtttccg cgatgactac
    36781 caatgtggca cgtcgtaaag agaaccacct cccggcactt tatcagcagc tgtttttgct
    36841 gatgaataag ttcccagggg atttgccgaa atttcgcctg gcgctgacga tgattatcgc
    36901 gcaccagcgc ctgcgtgatg cgccgatccc ggttaacgag gatttatcgg cgtttcaccg
    36961 acaaatgcgc cgcacagcag accatgtgat atctgcccgt agcgatgata aacgtcgtcg
    37021 gtactttggc cagttgctgg aagaactgga aatctaccag gaaaagctac gcatctggca
    37081 agcgccaccg caggtgacgg aaccggttca tcggctggcg gggatgctcc ataagtatca
    37141 acatgcgttg accgatagtt aagtcaaaac cgacgccaaa agcgtcggtt ttttcatggc
    37201 tatacttagc gatgcacggc agaactcgcc gcgaaacgtg acggtggcaa cagatgaata
    37261 tttatacctt tgattttgat gagattgaga gtcaggagga tttttatcgc gacttcagcc
    37321 aagcctttgg tctggcgaaa gataaggtac gcgatctcga ctcactatgg gatgtgttaa
    37381 tgaacgatgt cctgccgcta ccacttgaga ttgaatttgt tcatctggga gagaaaacgc
    37441 gtcgccgttt tggcgcgtta atattgctgt ttgatgaggc agaggaagag ctggaagggc
    37501 atttgcgttt taatgttcgt cattagcgca aaaaaaagcc cccgaaccgg gggcaatatc
    37561 gtcggacaag acgatgaggg tttatttgta cagttcagcc gtagcgtgcc aggtgtcacc
    37621 gctacgagct tcagtaatct ggtaggccgt tgcgcctttc tcttccgctt ttttgttcag
    37681 catttcacgc atatccattg gcgaagacgc cacaccactt acggatacgg tcccgattgc
    37741 ttcacgattt tgtgcttgtg cagcatcaat ggagtcggca gcgaatgcac cgaaagagag
    37801 aacagaaagt acgcttaatg cagctacagt ggttttgatt ttcatgattt ttacctcgac
    37861 ataatctttt agctgggcct ttgtttcgtg acccatatca caaaatcaag tatacactaa
    37921 ttattgaact aattaatacc acgc
//
LOCUS       DAZGSP010000045        37014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZGSP010000045 DAZGSP010000000
VERSION     DAZGSP010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.045"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..748
                     /locus_tag="ACHK74_002440"
     CDS             <1..748
                     /locus_tag="ACHK74_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0221248.1"
                     /translation="GGGFSDNYTHVQVGADKKHELDGLDLFTGVTMTYTDSNAGSDTF
                     SGKTKSVGAGLYASALFDSGAYIDLIGKYVHHDNEYTASFAGLGTKDYSSHSWYAGAE
                     VGYRYHVTEDAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVDVGK
                     SFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGGKDGRMLMSVGLNAEVRD
                     NIRFGLEFEKSAFGKYNVDNAVNASFRYSF"
     gene            complement(1315..3987)
                     /gene="rcsD"
                     /locus_tag="ACHK74_002441"
     CDS             complement(1315..3987)
                     /gene="rcsD"
                     /locus_tag="ACHK74_002441"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFP0221249.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            4725..5816
                     /gene="ompC"
                     /locus_tag="ACHK74_002442"
     CDS             4725..5816
                     /gene="ompC"
                     /locus_tag="ACHK74_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP0221250.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGRGYDDEDILKYVDVGATYYFNKNMSTYVDYK
                     INLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            5928..6983
                     /gene="apbE"
                     /locus_tag="ACHK74_002443"
     CDS             5928..6983
                     /gene="apbE"
                     /locus_tag="ACHK74_002443"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFP0221251.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQTAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            7057..8121
                     /gene="ada"
                     /locus_tag="ACHK74_002444"
     CDS             7057..8121
                     /gene="ada"
                     /locus_tag="ACHK74_002444"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFP0221252.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADSEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADHVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            8121..8771
                     /gene="alkB"
                     /locus_tag="ACHK74_002445"
     CDS             8121..8771
                     /gene="alkB"
                     /locus_tag="ACHK74_002445"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFP0221253.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     ATTAAGYPDFQPDACLINRYAAGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            8847..10490
                     /gene="yojI"
                     /locus_tag="ACHK74_002446"
     CDS             8847..10490
                     /gene="yojI"
                     /locus_tag="ACHK74_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFP0221254.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            10708..12354
                     /gene="mqo"
                     /locus_tag="ACHK74_002447"
     CDS             10708..12354
                     /gene="mqo"
                     /locus_tag="ACHK74_002447"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFP0221255.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(12503..13012)
                     /gene="eco"
                     /locus_tag="ACHK74_002448"
     CDS             complement(12503..13012)
                     /gene="eco"
                     /locus_tag="ACHK74_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFP0221256.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(13126..13290)
                     /gene="yojO"
                     /locus_tag="ACHK74_002449"
     CDS             complement(13126..13290)
                     /gene="yojO"
                     /locus_tag="ACHK74_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFP0221257.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            13399..13893
                     /gene="napF"
                     /locus_tag="ACHK74_002450"
     CDS             13399..13893
                     /gene="napF"
                     /locus_tag="ACHK74_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFP0221258.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            13883..14146
                     /gene="napD"
                     /locus_tag="ACHK74_002451"
     CDS             13883..14146
                     /gene="napD"
                     /locus_tag="ACHK74_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFP0221259.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            14143..16629
                     /gene="napA"
                     /locus_tag="ACHK74_002452"
     CDS             14143..16629
                     /gene="napA"
                     /locus_tag="ACHK74_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFP0221260.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            16636..17331
                     /gene="napG"
                     /locus_tag="ACHK74_002453"
     CDS             16636..17331
                     /gene="napG"
                     /locus_tag="ACHK74_002453"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFP0221261.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            17318..18181
                     /gene="napH"
                     /locus_tag="ACHK74_002454"
     CDS             17318..18181
                     /gene="napH"
                     /locus_tag="ACHK74_002454"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFP0221262.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            18178..18627
                     /gene="napB"
                     /locus_tag="ACHK74_002455"
     CDS             18178..18627
                     /gene="napB"
                     /locus_tag="ACHK74_002455"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFP0221263.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            18637..19239
                     /gene="napC"
                     /locus_tag="ACHK74_002456"
     CDS             18637..19239
                     /gene="napC"
                     /locus_tag="ACHK74_002456"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFP0221264.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            19252..19875
                     /gene="ccmA"
                     /locus_tag="ACHK74_002457"
     CDS             19252..19875
                     /gene="ccmA"
                     /locus_tag="ACHK74_002457"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFP0221265.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            19872..20534
                     /gene="ccmB"
                     /locus_tag="ACHK74_002458"
     CDS             19872..20534
                     /gene="ccmB"
                     /locus_tag="ACHK74_002458"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFP0221266.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            20576..21313
                     /gene="ccmC"
                     /locus_tag="ACHK74_002459"
     CDS             20576..21313
                     /gene="ccmC"
                     /locus_tag="ACHK74_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFP0221267.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            21310..21519
                     /gene="ccmD"
                     /locus_tag="ACHK74_002460"
     CDS             21310..21519
                     /gene="ccmD"
                     /locus_tag="ACHK74_002460"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFP0221268.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            21516..21995
                     /gene="ccmE"
                     /locus_tag="ACHK74_002461"
     CDS             21516..21995
                     /gene="ccmE"
                     /locus_tag="ACHK74_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFP0221269.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            21992..23935
                     /gene="ccmF"
                     /locus_tag="ACHK74_002462"
     CDS             21992..23935
                     /gene="ccmF"
                     /locus_tag="ACHK74_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFP0221270.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            23932..24489
                     /gene="dsbE"
                     /locus_tag="ACHK74_002463"
     CDS             23932..24489
                     /gene="dsbE"
                     /locus_tag="ACHK74_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFP0221271.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            24486..25538
                     /gene="ccmH"
                     /locus_tag="ACHK74_002464"
     CDS             24486..25538
                     /gene="ccmH"
                     /locus_tag="ACHK74_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFP0221272.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(25573..26220)
                     /gene="narP"
                     /locus_tag="ACHK74_002465"
     CDS             complement(25573..26220)
                     /gene="narP"
                     /locus_tag="ACHK74_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFP0221273.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            26540..29131
                     /gene="yejO"
                     /locus_tag="ACHK74_002466"
                     /pseudo
     CDS             26540..29131
                     /gene="yejO"
                     /locus_tag="ACHK74_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(29235..29311)
                     /locus_tag="ACHK74_002467"
     tRNA            complement(29235..29311)
                     /locus_tag="ACHK74_002467"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29275..29277),aa:Pro,seq:ggg)
     gene            complement(29386..31146)
                     /gene="yejM"
                     /locus_tag="ACHK74_002468"
     CDS             complement(29386..31146)
                     /gene="yejM"
                     /locus_tag="ACHK74_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFP0221274.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            complement(31166..31393)
                     /gene="yejL"
                     /locus_tag="ACHK74_002469"
     CDS             complement(31166..31393)
                     /gene="yejL"
                     /locus_tag="ACHK74_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFP0221275.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            31575..32582
                     /gene="yejK"
                     /locus_tag="ACHK74_002470"
     CDS             31575..32582
                     /gene="yejK"
                     /locus_tag="ACHK74_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFP0221276.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            complement(32721..33005)
                     /gene="rplY"
                     /locus_tag="ACHK74_002471"
     CDS             complement(32721..33005)
                     /gene="rplY"
                     /locus_tag="ACHK74_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFP0221277.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(33130..34890)
                     /gene="radD"
                     /locus_tag="ACHK74_002472"
     CDS             complement(33130..34890)
                     /gene="radD"
                     /locus_tag="ACHK74_002472"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP0221278.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHEHDEKGEWLKITYYDEDSADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            35040..35735
                     /gene="rsuA"
                     /locus_tag="ACHK74_002473"
     CDS             35040..35735
                     /gene="rsuA"
                     /locus_tag="ACHK74_002473"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFP0221279.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            35763..36953
                     /gene="bcr"
                     /locus_tag="ACHK74_002474"
     CDS             35763..36953
                     /gene="bcr"
                     /locus_tag="ACHK74_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFP0221280.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSHFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
BASE COUNT         8618 a         9062 c        10190 g         9144 t
ORIGIN      
        1 cggcggtggc ttcagtgaca actacacaca cgttcaggtg ggggcggata aaaaacacga
       61 actggacggt ctggacctct tcaccggggt gaccatgaca tataccgaca gcaacgccgg
      121 cagcgacacc ttcagtggta aaacgaagtc agtgggtgcg ggtctgtatg cttcagccct
      181 gtttgattcc ggggcctata tcgacctgat tggtaagtat gttcaccatg ataatgagta
      241 tacggcgtcc tttgccggac tcggcacaaa agactacagc tctcattcct ggtatgccgg
      301 tgctgaagtg ggttaccgct atcatgtcac agaggatgcc tggattgagc cacaggcgga
      361 actggtctat ggtgctgtct ccgggaaaca gttcagctgg aaggaccagg gaatgaacct
      421 caccatgaag gataaggact ttaatccgct gattggccgt accggtgtgg atgtgggtaa
      481 atctttctcc ggtaaggact ggaaagtgac ggcacgtgct ggtcttggct atcagtttga
      541 cctgctggcg aacggagaaa cggtactgcg tgatgcatcc ggagagaagc gtattaaggg
      601 cgggaaagac ggccgtatgc tgatgtctgt gggtctgaac gcagaggtcc gtgacaacat
      661 ccgttttggt ctggagtttg agaaatcagc ctttggtaag tacaacgttg ataatgctgt
      721 caacgccagc ttccgttatt cattctgacg gttgttactg aaggggatta tggcctgacg
      781 gctccctgta aataaagaca ggcacagaag attgtgagtt acttagtgtt ccaccaacaa
      841 gtaaggtcgt aactctgtgc ctgcttcgta agcgtacagt gagggccatc gtcataactg
      901 aagcatccga cgacgtggtg tctaccagag tgtcagatat gcaggaaaat atgagctccg
      961 acagaaatgc agtaaaaaaa gagagtctcg tacacccctc cagaaaactc cagggctgta
     1021 agttgtatta aaaagctgat ttttctttag cgatacagac ctcaataagt ataacaccct
     1081 tcagttactg tggttatgca caggtgttat ttttaactgt gtggataaac agtgtcgatt
     1141 attcagattg gttatggggc atgtatggga catttttaat cggcgaaata cgtcgaagtt
     1201 cgtcgatatg gtaaacgaat catctatcca acccttgaaa aacggcgctc ctggacgatc
     1261 ttcgtcgatt tttaaaatgc aacaagtacg ttgttcatgt aataggctac cttgctacag
     1321 caagctcttg acataactgt caatgtcgct gatgtatttt tctattcctg gaacatcctt
     1381 ctcacgaatc agatgttcca gcgtttcaca taactgcttg ccgggtacca gatttagcat
     1441 ggcaaatacg cctttaagac gatgagccgt ttgggctaac gcagcaaagt cactggttgc
     1501 tgcttcagta tacagcctct taacatcatc cggtactgtg tctacaaaga gcgcataata
     1561 gccgctggca tggagttgcg cattttcatc tccgcccaga ggcgattctg tcacctcttc
     1621 ctgcgccagt tgcacttcaa ttaattgtaa gaccgcttcc tgcatagcgt tgctcatatt
     1681 gaagttgacg cacaattgac caggcccaat ttcccgtacg ccagactcat catcgcttaa
     1741 aagcaagcca gaggcagtaa gattagacgg attatccgtt aaaaagatat cataatcttg
     1801 actaattaat ctttcatcgg gtgtgataca ggttgcaccc caattttcta actggcgagt
     1861 gacaatattc cgaatttctg ccgaagtaac atccaccatt acgcagacat catccagtaa
     1921 acgctcttct tcctcttcaa cttccgggtc agcggcgagc attttgatat gcacagagta
     1981 gcgtgtacca agcccatccc gcgttttgat gtttaaatga ccgcccagtt tacgcgccag
     2041 ttgatcgctc agccagaatg ccagcgggtc cgccttgcca tagcgatcgt tttgggtctg
     2101 gttgataaac gggaagtgca aattatccat ttcatgaata cttacgcctt ctccggtgtc
     2161 cagaatgcgg aatgtcaggc ggtcttcaga ggactcatcc tgatcaacct caagggtgat
     2221 ttttcccaat tgcgttgagg tcacggcata ttgcatcagt agcagcaaaa tacgtcgtaa
     2281 ggcatcgcga tcgccgcggc gcatatcgtg tgctttcaga tgattgttaa tcagcagttg
     2341 cagaccttta cgcttgatgg caggcaacac tgaaggcaca acttcatcaa ttaaatcctg
     2401 cacggagaac agcaccgtct cacttttcca gctatcatcc gcaagcatgt tcgctaactg
     2461 tatttcatcg accagccgca ccagcacatc tgcctgattc gccagttgtt tgctttccgg
     2521 ggcgttgagt ttagccgcgc tctccgccag ggactgtgcg ggttctttca gcgcatcgcc
     2581 aatgtttttc ataaaggtca tccggccctg ctggtttttc tcatacagac gctgcgcctg
     2641 cttgagtttc ttgtttacca gcacttcgcg atcctgatcg cgaataatga aaatttgtgt
     2701 gcgcggcgcg acctggctgc ggaacatgcg gatctcatac agctcgttat tgatcgtcgc
     2761 ctgaataatc ccctgatgct gttccgccat ggtggtgatg ttttgcagat tcaaatgcgg
     2821 cagcaaatga tcggcaattt tgttacttat gacagtgcgg ttcgattcct gatcgtgaac
     2881 cagcaggccg agcggcagca gtgagactat ctcttcattg attgcccgta aaatgcgcaa
     2941 ttcgttattg accgccgtgt tgggtagact ttctgtactg cgactggaga aatggcggaa
     3001 tgtggtatag ccaaataacg ccagcgccag caaaccgatg ttcagcagca gtggcagcag
     3061 aatgttttgc aacgtatcca gcaataaggt gccataagga acctgccaga ccaggcgcat
     3121 atcggtagag ttgagtgccg aggagatttc aatcttcgta ctgttaaagt ggatactgac
     3181 gctatccgtt ccttctttct cattatcatt gtctcccgtt gccgtcgtgt ctggctcaag
     3241 gcggaaactg tccagcggca tacccggtgg gatcaaatca ttaatcggca gatcaaaagc
     3301 cacgaccgtt gccagatgtc ctggctggtt aaaggtagta cgcaaggtaa agtaatgacc
     3361 gttctgccag gccaggcggc gcatgttaga aaagctttcg cgttcatcga gggcgttggc
     3421 ctgttgcaac atctctgcac gacgtgaatc aacaatgtcg ctgacggtcg attctttaaa
     3481 tccggaggtg agatctttaa ggggtagggt tgagatcagc accagactgt tatcctgacc
     3541 attcaggtaa tacatcgacc acggtacatt ttctgcgccc cacaatgtat ccagataagt
     3601 ggacatccgc tgagtcatct caagcgttga gttgtcgtga gagccaaaga ttaacgcttc
     3661 cgttttgcgg cgtggtttct ccagatagta gacatcctgt ttcaggcgcg tctcttgtaa
     3721 accttcgccg gaggaggggg aggtcgtcgc ggcaatgttg tcgtagatct gccaggtcac
     3781 gtaacgccag gtatcgacgc gcttttggat tgcgtgggta atgtcgacaa tctggtaact
     3841 tttatctttc agccatgcgt taacggcgct ttgtaccatt acccccatcg tcaccagtaa
     3901 cacaatgatc aacagtaaaa agaagcgggt aatgctcccc ggtaggagtg aaaagcgggt
     3961 cgtggccgtt gtctctttct gacgcattcg tgtttatgac ctgttaaaac ttcgcgaagt
     4021 gatagggcag tataaagggt acagtgtgaa attccagact cttacctgat tgatgattct
     4081 ctattctccc gtgatattta tcatcctgta tgggggaaat agatgtacaa gcgccatttt
     4141 gctgtacaaa taacctacaa aaagcccaac gaaattaatt gcaaataaac gaagattgct
     4201 ggaaattatg ctgatgttaa ttatttgtga aatagttaac aagcgttata gtttttctgt
     4261 ggtagcacag aataatgaaa agtgtgtaaa gaagggtaaa aaaaaccgaa tgcgaggcat
     4321 ccggttgaaa taggggtaaa cagacattca gaaatgaatg acggtaataa ataaagttaa
     4381 tgatgatagc gggagttatt ctagttgcga gtgaaggttt tgttttgaca ttcagtgctg
     4441 tcaaatactt aagaataagt tattgatttt aaccttgaat tattattgct tgatgttagg
     4501 tgcttatttc gccattccgc aataatctta aaaagttccc ttgtatttac attttgaaac
     4561 atctatagcg ataaatgaaa catcttaaaa gttttagtat catattcgtg ttggattatt
     4621 ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc agtatgcagt
     4681 ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa gttaaagtac
     4741 tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct gaagtttaca
     4801 acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac tatttctctg
     4861 acaacaaaga tgtagatggc gaccagacct acatgcgtct tggcttcaaa ggtgaaactc
     4921 aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag ggcaacagcg
     4981 ctgaaaacga aaacaactcc tggacccgtg tggcattcgc aggtctgaaa ttccaggatg
     5041 taggttcttt cgactacggt cgtaactacg gcgttgttta cgacgtaact tcctggaccg
     5101 acgtactgcc agaattcggt ggcgacactt acggttctga caacttcatg cagcagcgtg
     5161 gtaacggttt cgcgacctac cgtaacactg acttcttcgg tctggttgac ggcctgaact
     5221 ttgctgttca gtaccagggc aaaaacggta gcgtaagcgg cgaaggcatg actaacaacg
     5281 gtcgtgaagc actgcgtcag aacggcgacg gcgttggcgg atctatcact tatgattacg
     5341 aaggcttcgg tatcggtgct gcagtttcca gctccaaacg tactgatgat cagaacagcc
     5401 cgctgtacat cggtaacggc gaccgtgctg aaacctacac tggtggtctg aaatacgacg
     5461 ctaacaacat ctacctggct gctcagtaca cccagaccta caacgcaact cgcgtaggtt
     5521 ccctgggttg ggcgaacaaa gcacagaact tcgaagctgt tgctcagtac cagttcgact
     5581 ttggtctgcg tccgtccctg gcttacctgc agtctaaagg taaaaacctg ggtcgtggct
     5641 acgacgacga agatatcctg aaatatgttg atgttggtgc gacctactac ttcaacaaaa
     5701 acatgtccac ctatgttgac tacaaaatca acctgctgga cgacaaccag ttcactcgtg
     5761 acgctggcat caacactgat aacatcgtag ctctgggtct ggtttaccag ttctaatctc
     5821 gattgatatc gaacaaaggg cctgcgggcc cttttttcat tgttttcagc gtacaaacac
     5881 agttttttgg tgtactcttg cgaccgttcg catgaggata atcacgtatg gaaataagct
     5941 ttacccgcgt ggcactgctg gctgccgcgc tcttctttgt tggttgcgat caaaaaccac
     6001 aaaccgccaa aacccacgct actgaagtta ccgttcttga aggcaaaact atgggtacct
     6061 tctggcgtgc cagcatcccg ggcattgacg ccaaacgcag tgccgaactt aaagaaaaga
     6121 ttcagaccca gctggacgcc gacgatcagc tgctttcgac ctataaaaaa gattccgcgc
     6181 tgatgcgctt taacgattcg caaagtttat cgccgtggcc ggtaagtgaa gcgatggccg
     6241 atatcgtcac cacctcgctg cgcattggcg caaggaccga tggtgcgatg gatataaccg
     6301 tcgggccgct ggtgaatctg tggggctttg gcccggaaca acagccggtt cagattccga
     6361 gccaggaaca gatcgatgcg atgaaagcca aaaccggctt acagcacctg acggtcatta
     6421 atcagtcgca tcagcaatat ctgcaaaaag acctaccgga tttatatgtc gatctctcca
     6481 ccgtcggtga aggttatgcg gcggatcacc tggcacgctt gatggagcag gaagggattt
     6541 cccgctatct ggtgtcggtg ggcggcgcgc tgaacagccg tggtatgaac ggtgaaggcc
     6601 tgccgtggcg ggtagcgatt caaaaaccaa ccgataaaga aaacgcggtt caggctgtgg
     6661 tggatatcaa cggtcatggt attagcacct ctggcagtta ccgtaactat tacgaactgg
     6721 acggcaaacg tctttctcat gttatcgatc cgcaaaccgg gcgtcccatc gaacacaatc
     6781 tggtatccgt gacggtgatt gctccgacgg cgctggaagc cgatgcctgg gatactggct
     6841 tgatggtact cgggccggag aaagccaaag aggttgttcg ccgggaaggg ctggcggttt
     6901 atatgatcac caaagaaggc gatagcttta aaacctggat gtcaccacag tttaaaagct
     6961 tccttgtcag cgaaaaaaat taaagcgcaa gattgctggt ttttgcgtga tggtgaccgg
     7021 gcagactaaa ggctatcctt aaccagggag ctgattatga aaaacgccac atgcttaact
     7081 gacgatcaac gctggcaatc tgtcttagcc cgcgacccga atgccgacag cgaattcgtt
     7141 ttcgccgtgc gcaccacagg catcttttgc cgtccgtctt gccgcgccag acatgctttg
     7201 cgggaaaacg tctccttcta cgcaaatgcc agcgaggcac tcgccgctgg ctttcgcccc
     7261 tgcaaacgtt gtcagccaga caaagccaat gcccagcaac atcggttgga taaaattacc
     7321 cacgcctgtc gactgctgga acaggaaacg cctgtaacgc tggaagcctt agccgaccat
     7381 gtggcgatga gtccgttcca tctgcatcgg ttgtttaaag cgactaccgg aatgacgcct
     7441 aaagcctggc aacaggcctg gcgcgctcgc cgtttgcgcg aatcgctggc gaaaggggag
     7501 agcgtgacga cgtctattct taacgccgga ttccccgaca gcagcagtta ctaccgcaaa
     7561 gccgacgaaa cgctgggcat gacggctaaa caattccgtc acggtggcga aaatctggcg
     7621 gtgcgttacg cgctggctga ttgtgagctg ggtcgttgcc tggtggcaga aagcgagcgg
     7681 gggatttgcg cgatattgct gggcgatgat gacgcgacac taatcagcga gttgcagcag
     7741 atgtttcccg ctgccgacaa cgcgcctgcc gatctgatgt ttcagcaaca tgtgcgtgaa
     7801 gtgatcgcca gcctcaatca acgcgatacg ccgctgacgt taccgctgga cattcgcggc
     7861 actgcttttc agcaacaagt ctggcaggca ctgcgcacga taccttgcgg tgaaaccgtc
     7921 agttatcagc aactggctaa cgccatcggc aaaccgaaag cggtacgggc cgttgccagc
     7981 gcctgtgccg ccaacaagct ggctatcata ataccctgtc atcgggtggt ccgtggtgat
     8041 ggcacacttt ccggttaccg ctggggcgtg tcgcgtaaag cgcaactgct gcgccgcgaa
     8101 gctgaaaatg aggagaggta atgttggatc tgtttgccga tgctgaaccg tggcaagagc
     8161 cactggcggc tggtgcggta attttacggc gttttgcttt taatgctgcg gagcaactaa
     8221 tccgcgatat taatgacgtt gccagccagt cgccgtttcg ccagatggtc acccccgggg
     8281 gatataccat gtcggtggcg atgaccaact gtgggcatct gggctggacg acccatcggc
     8341 aaggttatct ctattcgccc attgatccgc aaacaaataa accgtggccc gccatgccac
     8401 aaagctttca tgatttatgt caacgtgcga ctacggcggc gggctatcca gatttccagc
     8461 cagatgcttg tcttatcaac cgctacgctg ctggcgcgaa actgtcgctg catcaggata
     8521 aagacgaacc ggatctgcgc gcgccgattg tttctgtttc tctgggctta cctgcgattt
     8581 ttcaatttgg tggcctgaaa cgtaatgatc cgctcaaacg tttgttgttg gaacatggcg
     8641 atgtggtggt gtggggcggt gagtcgcggc tgttttatca cggtattcaa ccgttgaaag
     8701 cggggtttca tccactcacc accgactgcc gctacaacct gacattccgt caggcaggta
     8761 aaaaagaata aaaataagaa ttattattgc tgtgcgcgcg aagatgttta aactgcgggc
     8821 tgtaattcat tgtccgggtt ttctgcatgg aacttcttgt acttgtctgg cggcagtatc
     8881 gctggccatt tatcagtgtg atggcgctaa gcctcgccag tgcggcatta ggcattggct
     8941 taattgcttt tatcaatcag cgccttatcg aaacggcgga taccagtctg ctggtgttgc
     9001 cggagtttct gggattattg ctgctgttga tggcagtcac tctcggatcg caactggcgc
     9061 tcaccacttt ggggcatcac ttcgtttacc gactgcgtag tgaatttatc aagcggattc
     9121 tggataccca cgtcgagcgc attgaacaac tcggtagcgc ctcgttgctg gcgggattaa
     9181 ccagcgatgt gcgcaatatc accattgctt ttgtgcgtct tccggaactg gtgcagggga
     9241 tcattctcac tatcggttcc gcggcgtatc tgtggatgct gtcgggcaaa atgttgctgg
     9301 taacggctat ctggatggcg atcaccatct ggggtggttt tgtactggtg gcgcgggtgt
     9361 acaaacatat ggcgaccctg cgtgaaaccg aagacaagct gtacacggat tttcaaaccg
     9421 tactcgaagg gcgcaaagag ctgactctga accgggaacg cgccgagtat gtgtttaaca
     9481 acctctacat ccccgatgcg caagagtatc gccatcatat tattcgcgca gacaccttcc
     9541 atcttagcgc cgtgaactgg tcaaacatca tgatgctggg cgctatcggc ctggtgttct
     9601 ggatggcgaa cagtctcggt tgggctgata ccaacgttgc tgcgacctat tcgttgacgc
     9661 tgttattcct gcgtacgccg ctgctttcgg cggttggcgc attgccgacg ctgctgacgg
     9721 cgcaggtggc gtttaacaag ctgaacaaat tcgcgctcgc gcctttcaaa gctgagtttc
     9781 cgcgcccgca ggcttttccc aactggcaaa cgctggagct gcgtaacgtg acgtttagct
     9841 atcaggataa cgcgttttcc gttgggccga ttaacctcac cattaaacgt ggcgagctgc
     9901 tgtttctgat tggtggcaac ggtagtggaa aatcgacgct ggcgatgttg ctgacgggct
     9961 tgtaccagcc acaaagcggc gaaatcttgc tggatggcaa acctgtcagt ggcgaacaac
    10021 cggaagatta tcgcaaactg ttttcggcag tgtttaccga tgtctggctg tttgatcaac
    10081 tgctggggcc ggagggtaaa cccgctaacc cgcaactggt tgagaagtgg ctggcgcagc
    10141 tgaaaatggc tcataagctt gagttaagca acgggcgtat tgttaacctg aagttatcaa
    10201 aagggcagaa aaaacgcgtg gcgctgttgc tggcgctggc agaagaacgc gatattatcc
    10261 tgctggatga atgggcggcg gatcaggatc cacacttccg tcgtgagttt tatcaggtgt
    10321 tgctgccgct gatgcaggag atgggtaaaa ctattttcgc tatcagtcat gatgatcatt
    10381 actttatcca cgccgaccgc ctgctggaaa tgcgcaatgg gcaacttagc gagctgacgg
    10441 gcgaagagcg cgatgccgct tcgcgtgatg ccgttgcccg gacggcataa tattacgacg
    10501 ctgacctggc ggactgctgc cgtcaggtca atatgctaaa aatcccttca tttctaataa
    10561 ccctataatt aatcaacgaa attataatgt ttctaaaatt agaatataat ttataaacat
    10621 tatttaaatg ttgttactta agtgttaccg ttgatgccgc gcaaatctca cctgcaataa
    10681 agcgactaaa agtaaggcat taacaagatg aaaaaagtga ctgccatgct cttctcgatg
    10741 gccgtggggc ttaatgccgt ttcgatggcg gcaaaagcga aagcgtccga ggagcaggaa
    10801 actgatgtac tgttgattgg cggcggcatt atgagcgcca cgttggggac ctatttacgc
    10861 gagctggagc ctgaatggtc gatgaccatg gtggagcgcc tggagggtgt cgcgcaggag
    10921 agttcgaacg gctggaataa cgccggaacc gggcattctg cactgatgga actgaactac
    10981 accccgcaaa acgccgatgg cagcatcagt attgaaaaag cagtcgccat taacgaagca
    11041 tttcagattt cccgccagtt ctgggcgcac caggttgagc gcggcgtgct gcgtactccg
    11101 cgttcattta tcaataccgt tccgcatatg agctttgtct ggggcgagga taacgttaat
    11161 ttcctgcgcg cccgttacgc cgcgttgcaa caaagctcgc tgtttcgcgg tatgcgttac
    11221 tctgaagatc acgcgcagat caaagagtgg gcaccgttag tgatggaagg gcgcgatcca
    11281 caacagaaag tagcggcaac acgcacagaa attggcactg atgtgaacta tggcgagatc
    11341 acccgccagt taattgcttc cttacagaag aaatctaact tctcgctgca actcagcagc
    11401 gaagtccgcg ccctaaagcg taatgacgat aacacctgga ccgttaccgt tgccgatctg
    11461 aaaaacggca ctgcacagaa catccgcgcc aaatttgtct ttatcggcgc gggcggtgcg
    11521 gcgctgaagc tattacagga atcggggatt ccggaagcga aagactacgc cggtttcccg
    11581 gtgggcggac agttccttgt ttcggaaaac ccggacgtgg ttaatcacca tctggcgaag
    11641 gtttacggta aagcatccgt tggcgcacca ccgatgtcgg ttccgcatat cgatacccgc
    11701 gttctggacg gtaaacgcgt agtgctgttt gggccatttg ccaccttctc aaccaaattc
    11761 ctcaaaaacg gttcattgtg ggatctgatg agttccacca ccacctctaa cgtgatgccg
    11821 atgatgcacg tcgggctgga taatttcgat ctggtgaaat atctggtgag tcaggtgatg
    11881 ttgagtgaag aggatcgttt tgaagcgttg aaagagtact atccgcaggc aaaaaaagag
    11941 gactggcgtt tgtggcaagc ggggcagcgc gtgcagatta tcaagcgtga tgccgagaaa
    12001 ggtggcgtac tgcgtctggg tactgaagtg gtcagtgacc agcaagggac cattgccgcg
    12061 ctgctggggg catcgccagg ggcgtcaacc gccgcgccga ttatgttgga tctgctggaa
    12121 aaagtatttg gcgatcgcgt ttccagcccg caatggcagg ctacgttgaa agcgatcgtt
    12181 ccgtcttatg gacgcaagct gaacggtgat gtagcggcaa cagaacgcga gttgcagtac
    12241 accagcgaag tgctggggct gaaatacgac aagccgcagg cagcagatag tacgccaaaa
    12301 ccgcagttga aaccgcaacc cgttcaaaaa gaagtggcgg atattgcgtt gtaatgatgt
    12361 gccacatccg gcatggtatg ccggatgtgg cgtatgctga taagacgcgc cagcgtcgca
    12421 tcaggcaccg gctcgggcgt tagatgtcgg atgcggcgtg accgccttat tcggcctacg
    12481 gggtgccgca cttcacagca ttttagcgaa ctaccgcgtt gtcaattttc tcttccgcct
    12541 tccagacgcg gtacttcaca tctacattgt ctggcgtata caccacgatc ggcagcttgc
    12601 tgttgtaacg cagcattcca gcatcgccca gatacgcggt gacaaatttc ttctctttct
    12661 tgccatccgg gcaggccatc atcgtggaaa ccggcgagct aactttatca aagacataat
    12721 aatcatagcc ccagccttcc agcgttttgc tttccagctt cccgccgaga cgatgcaaat
    12781 tgcaatcgac ttccagcgtc tgaccgatta acagttctac tttcagggta gattcatctt
    12841 cttgcggggt taactgaatc acctgacgct tcatcccttt ttcagcttgt ggataaggcg
    12901 cgattttttc cagtggctgg acgctttctg ccgcccaggc ggaagtggta gcgaaagcgg
    12961 caaacaatac tgcaggtaga atggtcttca tttttttgct ttccttcttc atttataggt
    13021 tttcaccgat gatgtcgctg aaggttaaca tattcatcag atttattaat ttgctttatg
    13081 aataacttaa agaacattca gacaatttta cctgctcttt tagtgttaaa ttctgatgag
    13141 ggagcaaacc agcagaaata tctctggtga attaatcgct atataaatat gtttataacc
    13201 atttgaaatg tgagcaaaag cccgtttttg ccacgcttcc cgccactctt ttgatcctgc
    13261 tacaggtttt accccgatcg gggtatgcat ctttgacaca tcctttaata tcttagcggc
    13321 tataaaaatg gcttattaat tatgcggttt atttggtcgc tctcaatttt cagagcgcgt
    13381 taatgatgga aggtcaatgt gaagattgat gcatcccgtc ggggcatact cactggtcgc
    13441 tggcgcaaag ccagtaacgg tatccgtcca ccctggtccg gtgatgaatc tcattttctg
    13501 acccattgca cccgttgcga cgcctgtatt aatgcttgtg aaaacaacat tctgcaacgc
    13561 ggcgcgggcg gctatccgag cgttaatttc aaaaataacg agtgcagctt ctgttacgcc
    13621 tgcgcgcagg cttgccccga atcgctattt tctccgcgcc acaccagggc ctgggatttg
    13681 cagttcacca ttggggacgc gtgcctggcg tatcagtcag ttgaatgccg ccgctgtcag
    13741 gatagctgtg agccaatggc aattatcttt cgcccgacgc tctccggaat ctaccagccg
    13801 caacttaata gccaactttg caacggatgt ggagcatgtg cagccagctg tccggtatca
    13861 gccataactg cggagtatct ccatgcacac taactggcaa gtttgcagcc tggtcgtgca
    13921 ggccaaaagc gaacgaattt cagacatcag cacccaactg aacgcctttc ccggctgtga
    13981 agttgctgtc agcgacgcgc cgagcggtca gttgattgtg gtggtggaag cagaagacag
    14041 cgaaacgctg atccaaacca ttgagtcagt acgcaacgta gagggcgtgc tggcggtgtc
    14101 gctggtttat caccagcagg aagagcaagg tgaggaaaca ccatgaaact cagtcgtcgt
    14161 agctttatga aagctaacgc cgttgcggcc gctgcggcgg ctgccggtct cagcgtgccg
    14221 ggcgttgccc gcgccgttgt tggtcagcag gaagccatta aatgggataa agcgccgtgc
    14281 cgtttctgcg gtactggttg cggcgttctg gtcggaacgc agcaggggcg tgtggtggcc
    14341 tgtcagggcg acccggacgc accggttaac cgtggcctga actgcattaa gggctatttc
    14401 ctgcccaaga tcatgtacgg taaagaccgt ttgacgcagc cgctgctgcg tatgaaaaac
    14461 ggtaaatatg acaaagaagg cgaatttacc ccaatcacct gggatcaggc cttcgatgtg
    14521 atggaagaga aattcaaaac cgccctgaaa gaaaaaggac cggaatcgat cggtatgttc
    14581 ggttctggtc agtggactat ctgggaaggt tatgccgcgt ccaagctgtt caaagcgggc
    14641 ttccgttcga acaacatcga cccgaacgca cgtcactgta tggcgtcggc agtagttggc
    14701 tttatgcgta cctttggtat ggatgagccg atgggctgct atgacgacat cgagcaggct
    14761 gacgcgtttg tgctgtgggg cgctaacatg gcggagatgc acccgatcct ctggtcacgc
    14821 atcactaacc gtcgtctctc taaccagaac gtcaccgtgg cggtgctttc tacctaccag
    14881 catcgtagct tcgagctggc ggataacggc atcatcttta cgccgcaatc tgacctggtg
    14941 atcctgaact acatcgccaa ctatatcatt caaaacaatg cgataaatca ggacttcttc
    15001 agcaagcacg ttaacctgcg caaaggggcg acggacatcg gctacggttt acgtccgacc
    15061 catccgctgg aaaaagcagc gaagaatcca ggttctgacg cctccgaacc gatgagcttt
    15121 gaagattaca aagccttcgt tgccgagtat acgctggaaa aaactgccga aatgaccggc
    15181 gtgccgaaag accagttaga acaactggcg cagctgtatg ccgatccgaa caagaaagtc
    15241 atctcctact ggacgatggg cttcaaccag catactcgtg gcgtgtgggc caacaacctg
    15301 gtctacaacc tgcacctgct gaccggcaaa atttcccagc cgggttgcgg tccgttctcc
    15361 ctgaccgggc agccttccgc gtgtggtact gcgcgtgaag tgggcacctt tgctcaccgt
    15421 ctgcctgcgg acatggtggt gactaacgag aaacaccgtg atatttgcga gaagaagtgg
    15481 aatatcccga gcggcaccat tccggcgaaa atcggtctgc acgcggtggc acaagaccgt
    15541 gcactgaaag acggcaagct gaatgtttac tggaccatgt gtaccaacaa catgcaggcc
    15601 gggccgaaca ttaatgaaga gcgtatgccg ggctggcgcg atccgcgcaa cttcatcatc
    15661 gtctccgatc cgtatccgac agtcagtgcg ctggcagccg acttgatcct gccgaccgca
    15721 atgtgggtag agaaagaggg cgcttacggt aacgccgaac gccgtactca gttctggcgt
    15781 cagcaggtac aggcgccagg cgaagcgaaa tcggatctct ggcagttagt ccagttctcc
    15841 cgccgcttca aaactgaaga agtatggccg gaagagctgc tggcgaagaa accggaactg
    15901 cgtggcaaaa cgctgtacga agttctgtat gccacccccg aagtgagcaa attcccggta
    15961 tccgaactgg cggaagatca gctgaacgat gaatcccgcg agctgggctt ctatctgcaa
    16021 aaagggctgt tcgaagagta cgcatggttt ggtcgcggtc acggtcacga tctcgcgccg
    16081 ttcgatgact accacaaagc gcgcggtctg cgctggccgg tggtgaacgg taaagaaaca
    16141 cagtggcgtt acagcgaagg taacgacccg tacgtgaaag cgggcgaagg ctataagttc
    16201 tacggtaaac cggatggcaa agcggtgatc ttcgcactgc cgttcgaacc ggcggcggaa
    16261 gcaccggatg aagagtacga cctgtggctc tctaccggac gcgttctgga gcactggcac
    16321 accggcagta tgactcgccg tgtgccggaa ctgcaccgcg ccttcccgga agcggtcttg
    16381 tttattcacc cgctggatgc gaaagcgcgc gatctgcgcc gtggcgacaa agtgaaagtg
    16441 gtttctcgcc gtggcgaagt gatctcgatt gttgaaacgc gcggtcgtaa ccgtccgcca
    16501 cagggcctgg tgtacatgcc gttcttcgac gccgcacagc tggttaacaa actgacgctg
    16561 gatgcgaccg atccgctctc gaaagagacg gacttcaaga agtgcgcagt caaactggag
    16621 aaggtgtaag ccattatgtc ccggtcagcg aaacctcaaa atggtcgccg ccgctttctg
    16681 cgcgatgtcg ttcgcacagc aggcgggctg gctgccgtgg gtgtggcgct ggggttacaa
    16741 cagcaaaccg cacgcgcatc tggcgtgcgg ttgcgcccgc ccggagccat aaacgagaac
    16801 gcctttgcca gtgcctgtgt gcgttgcggt cagtgtgttc aggcttgccc ttacgacacc
    16861 ttaaaactgg cgacgctggc ctctggtctg tcggcgggca cgccatattt tgtcgcacgg
    16921 gatattcctt gcgaaatgtg tgaggacatt ccgtgcgcca aagtgtgccc aagcggtgcg
    16981 ctggatcgtg agattgaatc gatcgacgac gcgcggatgg ggctggcggt actggtggac
    17041 caggaaaact gtctcaactt tcaggggctg cgctgcgatg tttgttatcg cgaatgcccg
    17101 aaaattgatg aggccatcac cctggagctg gagcgcaaca cgcgtaccgg taagcacgcc
    17161 cgctttctgc cgacggttca cagcgacgcc tgtaccggtt gcggtaagtg cgaaaaagta
    17221 tgcgtgctgg aacaaccggc aatcaaggtg ttaccgctgt cactggcgaa aggggagtta
    17281 ggtcaccatt accgcttcgg ttggctggag gggaacaatg gcaaatcgta aacgtgacgc
    17341 cgggcgcgag gcgctggaga aaaaaggctg gtggcgcagt caccgttggc tggtgttacg
    17401 tcgcctttgt cagttcttcg tgctggggat gtttttgagc ggtccatggt ttggtgtgtg
    17461 gatcttgcac ggcaactaca gcagtagcct gttattcgac accgttccgc tgaccgatcc
    17521 gctgatgacg ctgcaaagtc ttgccagtgg tcatctgcct gccacggtgg cgttgaccgg
    17581 tgcggtgatt atcaccgtgc tctacgccct ggcgggaaag cgattatttt gcagctgggt
    17641 ctgcccgctg aacccgatta ccgacttagc gaactggtta cgcaggcgct ttgacctcaa
    17701 tcagtctgcg acgatccctc gccacattcg ctacgtgctg ttggtggtca ttctgctggg
    17761 atcggcctta actggcacgc tcatctggga atggatcaac cccgtatctc tgatggggcg
    17821 cagcctggta atgggcttcg gcagcggcgc gctgcttatt ctcgcgctgt ttttatttga
    17881 tctactggtc gttgaacacg gctggtgcgg gcacatttgt ccggttggcg cgctgtatgg
    17941 cgtgctgggt agcaaaggtg tgattaccgt tgcggccacc gatcggcaga aatgtaaccg
    18001 ctgtatggat tgttttcatg tttgcccgga accgcatgtg ctacgtgccc cggtgctgga
    18061 tgaacaaagc ccggtgcagg tcaccagccg cgattgcatg acgtgcggtc gctgcgtgga
    18121 tgtctgttct gaggatgtat ttacaataac tacacgatgg agttcgggag cgaaatcatg
    18181 aaaagccatg acctgaagaa agcgctgtgt caatggacgg cgatgctggc cctggtggta
    18241 agcggcgcgg tttgggcggc taatggcgtc gattttagcc agtcgccgga agtatccggg
    18301 acacaggaag gggccattcg gatgccaaaa gagcaggaac ggatgccgct gaactatgtg
    18361 aatcagccgc caatgatccc gcatagcgtt gaaggttatc aggtaacgac caataccaac
    18421 cgctgcttgc agtgccacgg tgtcgaaagt tatcgcacca ctggcgcgcc gcgtatcagt
    18481 cctactcact ttatggacag cgacggcaaa gtgggcgcgg aagtggctcc gcgtcgttat
    18541 ttctgtctgc aatgtcacgt accgcaggcc gataccgcgc caatcgtggg gaataccttt
    18601 accccatcaa aaggttacgg gaaataagag gttattatgg gaaattctga ccgtaagcct
    18661 ggtctgatta agcgcctgtg gaaatggtgg cgtaccccca gccgtctggc gctggggacg
    18721 ctgctgttga tcggttttgt tggcggcatc gtcttctggg gtggctttaa caccgggatg
    18781 gaaaaagcca ataccgaaga gttctgcatt agctgccacg aaatgcgcaa cacggtgtat
    18841 caggaataca tggattccgt gcactacaac aaccgtagcg gcgtccgtgc gacctgtccg
    18901 gattgtcacg ttccgcacga gtttgtgccg aagatgatac gcaagctcaa agcaagtaaa
    18961 gagctgtatg gtaaaatttt tggcgttatt gacacgccgc agaaatttga agctcatcgt
    19021 ctgacgatgg cacagaatga gtggcggcgc atgaaggaca ataactcgca ggagtgccgt
    19081 aactgtcaca acttcgagta tatggataca accgcccaga aatcggttgc cgcgaagatg
    19141 catgaccagg cggtgaaaga tgggcaaacc tgtattgatt gccataaagg gatagcgcac
    19201 aagctgcccg atatgcgtga agtcgagcca ggtttttaac agggttattg cgtgggtatg
    19261 cttgaagcca gagagttact ttgtgagcgg gatgaacgaa ccttatttag tggcttgtca
    19321 tttacgctga acgcaggaga gtgggtacaa atcaccggta gcaacggcgc ggggaagaca
    19381 acgcttctcc gtttgctgac ggggttgtct cgccctgacg caggcgaggt tctctggcaa
    19441 gggcagccct tgcatcaggt acgcgacagc taccatcaaa acctgttatg gataggccat
    19501 cagccgggga tcaaaacccg gctgacggcg ttagaaaatc tgcactttta tcatcgcgat
    19561 ggcgataccg cacaatgtct ggaagccctg gcgcaggccg ggcttgccgg attcgaagat
    19621 attcctgtaa atcagctctc ggccgggcaa caacgccgcg tcgctttagc gcgtctgtgg
    19681 ctgacccgtg ccacgttatg gatcctcgac gagcctttta ccgcgattga cgttaacggc
    19741 gtcgatcgtc tgacccagcg tatggcgcag catacggagc agggggggat tgtgattctg
    19801 actacccacc agccgctcaa cgttgctgaa agtaaaattc gccgcatttc actgacgcaa
    19861 acgagggccg catgatgttc tggcgcattt tccgtcttga gctgcgtgta gcgtttcgcc
    19921 atagcgccga aatcgccaac ccgctgtggt tcttcctgat tgtaattacc ctttttccgc
    19981 tcagtatcgg tccggagccg caactgctgg cgcgtattgc accgggcatt atctgggttg
    20041 ctgcgctgct ttcatccttg ctggcgctgg aacgactgtt ccgtgacgat ttgcaggacg
    20101 gcagtcttga acaattgatg ttgttgccgt tacccttgcc cgccgttgtg ctggcgaagg
    20161 tgatggcgca ctggatggta accggtctgc cgttactcat cctttcgcca ctggtagcaa
    20221 tgctactggg aatggatgtt tatggctggc aagtgatggc gctgacgctt ctgctgggaa
    20281 cgcctacgct tggctttctc ggtgcaccgg gcgtggcgct gacagtggga cttaagcgcg
    20341 gtggtgtgct gctcagcata ctggtgttac cgctgactat cccattactc atcttcgcca
    20401 ccgccgcgat ggacgcggct tccatgcatt tgcccgttga cgggtatctg gcaattttag
    20461 gcgcgttgct ggcaggcacc gcgacattaa gtccttttgc gacggcggca gcgttacgaa
    20521 tcagcattca ataacgctgg attactgatt tttgttcgag tctggtatcg aaactatgtg
    20581 gaaaacactg catcaactgg cgatcccacc acggctgtat caaatctgtg gctggtttat
    20641 accgtggctg gcaattgcca gtgtggtcgt gcttaccgtc ggctggatct ggggattcgg
    20701 ctttgctccg gctgattatc agcagggaaa tagctaccgc attatctacc tgcatgtgcc
    20761 tgcggcgatc tggtcgatgg gcatttatgc atcaatggca gtggcagcgt ttattggcct
    20821 tgtctggcag atgaaaatgg ccaacctggc ggtggcggcg atggccccca ttggtgccgt
    20881 gtttaccttt attgccctgg ttaccggctc tgcatgggga aaaccgatgt ggggcacctg
    20941 gtgggtatgg gatgcacgtc tgacttctga actggtgctg ctttttttgt atgtgggtgt
    21001 gattgccctg tggcacgcct tcgacgaccg ccgtctggcg ggccgtgcgg caggtatcct
    21061 ggtgctgatt ggcgtggtga atctgccgat tattcattac tccgtggagt ggtggaacac
    21121 cctgcatcag ggatcaacgc ggatgcagca aagtatcgat ccggcgatgc gttcgccgct
    21181 gcgctggtcg atttttggct tcctgctcct gtctgccacg ctgacgctga tgcggatgcg
    21241 taatttgatt ttgctgatgg aaaaacgccg tccgtgggtg agtgaactga tactgaaaag
    21301 aggccgtaaa tgacccctgc atttgcttcc tggaatgaat ttttcgcaat gggcggttac
    21361 gccttttttg tctggctggc ggtggtgatg accgttattc cgctggtggt tttggtcgtg
    21421 cactcggtga tgcagcatcg cgcaattctg cgtggtgtgg cgcaacagcg ggcgcgtgag
    21481 gcgcgtttac gtgctgcgca acagcaggag gctgcatgaa tattcgccgt aaaaaccgct
    21541 tgtggattgc ctgtgctgtt ctggcagggt tggcactgac tatcggtctg gtgctatatg
    21601 cgctgcgctc gaatatcgat ctcttttata cgccggggga aattctctac ggcaagcgtg
    21661 aaactcagca aatgccggaa gtcggtcagc gtctgcgcgt tggcgggatg gtgatgccgg
    21721 gtagcgtgca gcgcgatccc aattcgctaa aagtaacttt caccatttac gacgctgaag
    21781 gttcagtaga tgtctcttac gaaggcattt tgccggatct gttccgtgaa gggcagggcg
    21841 ttgtggtgca gggcgaactg gaaaaaggca atcatatcct cgcaaaagaa gtgctggcga
    21901 aacacgacga aaactacacg ccgccagaag ttgagaaagc gatggaagcc aatcaccgtc
    21961 gcccggcgag tgtttataag gacccagcat catgatgccg gaaattggta acgggctgct
    22021 gtgtctggcg ctaggaattg cgctgctgct gtccgtgtat ccgctatggg gcgtagcgcg
    22081 cggagatgcg cgcatgatgg cgtcttcccg cttgtttgcc tggctgctgt ttatgtctgt
    22141 ggctggcgca tttctggtac tggtcaatgc cttcgtggtc aacgacttca ccgtcaccta
    22201 tgttgccagc aactccaata cccagcttcc ggtgtggtat cgcgtggcgg ctacctgggg
    22261 cgcgcatgaa ggctcgctcc tgctgtgggt gctgctgatg agcggctgga cctttgcggt
    22321 agcgattttt agtcagcgta ttccgctgga tattgtggcc cgcgtactgg cgataatggg
    22381 gatggtcagc gtcggctttt tgctgttcat tctctttacc tctaacccgt tctcacgcac
    22441 gttgccgaac ttcccgattg aagggcgcga tcttaacccg ctgttacagg atccggggct
    22501 gatcttccat ccgcctctgc tctatatggg gtacgtgggg ttctcggtgg cgtttgcttt
    22561 tgccattgct tctttgttga gcgggcgtct ggacagcact tatgcgcgtt ttactcgtcc
    22621 gtggacgctg gcggcgtgga tcttcttgac gctcggcatt gtgctcggtt ccgcatgggc
    22681 ctattacgaa ctcggctggg gcggctggtg gttctgggat ccggtagaaa acgcctcgtt
    22741 tatgccgtgg ctggtgggga ctgcgctgat gcactcactg gcggtcactg aacaacgcgc
    22801 cagcttcaaa gcgtggacat tactgctggc aatcagtgcc ttctcgttgt gtctgctggg
    22861 gactttcctg gtgcgttccg gcgtgctggt atcggtacac gcgtttgcct ctgatccggc
    22921 acgcggtatg tttatcctcg cctttatggt actggtgatt ggtggttcgc tgctgctgtt
    22981 tgccgcgcgt ggacacaaag ttcgctcacg cgtaaacaat gcgctgtggt cgcgggaatc
    23041 tctgctgtta gcgaacaacg ttttgctggt cgccgcgatg ctggtggtat tactggggac
    23101 gctgctgccg ctggtgcaca agcaactggg actgggcagt atttcgattg gcgaaccgtt
    23161 cttcaacacc atgtttacct ggctgatggt gccgtttgcg ctgctgcttg gtgtcggtcc
    23221 tctggtgcgc tgggggcgcg atcgcccacg taaaatccgc aatttattga ttatcgcctt
    23281 catctcaacg ctggtgctgt cgctgctgct gccgtggctg ttcgaaagca aagttgtggc
    23341 gatgacggtg ctcggcctgg caatggcctg ctggattgct gtgctggcaa ttgcggaagc
    23401 tgcgctgcgt atttcacgtg gcacgaaaac caccttcagt tattggggaa tggtggcggc
    23461 tcaccttggg ctggcagtga caattgttgg cattgccttt agtcagaact atagcgttga
    23521 gcgtgatgtg cgcatgaagt ccggcgatag cgtcgatatt catgaatatc gcttcacctt
    23581 ccgtgatgtc aaagaggtga ctggcccgaa ctggcgtggc ggtgtggcga ctatcggcgt
    23641 aacgcgagag ggtaaaccgg aaacagtgct gtatgcggaa aaacgttatt acaacactgc
    23701 cgggtcgatg atgaccgaag cggcgattga cggtggcatc acgcgtgacc tgtacgccgc
    23761 gctcggtgaa gagctggaaa acggcgcgtg ggctgtgcgt ctttactaca aaccatttgt
    23821 tcgctggatt tgggcgggcg ggctgatgat ggcgttgggc ggactgctgt gtctgtttga
    23881 tcctcgctat cgtaagcgcg tgagtccgca aaaaactgcg ccggaggctg tatgaagcgc
    23941 aaagtattgt taattccgtt gattatcttc ctggcgattg ccgcggcgct gctgtggcag
    24001 ttggcgcgta atgccgaagg ggatgatccg accaatctgg aatcggcgct cattggcaag
    24061 cctgtgccga agtttcgtct cgaatcactg gacaatccgg ggcagtttta tcaggcggat
    24121 gtgctgactc agggcaaacc agtgctgctt aacgtctggg cgacctggtg tccgacctgc
    24181 cgtgcggaac atcaatatct gaatcagctt tctgcgcagg gcatccgagt ggtcggcatg
    24241 aactataaag acgatcgcca gaaggcaatc agctggctga aagagctggg caatccttac
    24301 gcgctaagcc tgtttgatgg cgacggcatg ttagggctgg atctcggtgt ctatggcgcg
    24361 ccagaaacct tccttattga cggcaacggc atcattcgct atcgccatgc gggcgatctc
    24421 aatcctcgcg tctgggaaga agagatcaag ccgctgtggg agaaatacag taaggaggcc
    24481 gcacaatgag gtttttattg ggcgtgctga tgttgatgat ctccggctca gcgctggcga
    24541 ccatcgacgt gttgcagttt aaagatgaag cacaggaaca acagttccgt cagctcactg
    24601 aagaactgcg ctgcccgaaa tgccagaata acagcattgc cgattccaac tcgatgattg
    24661 ccaccgacct gcgccagaaa gtgtatgaac tgatgcagga aggtaaaagt aagaaagaga
    24721 ttgtcgatta tatggtggcg cgttacggca acttcgtcac ttacgatccg ccgttaacgc
    24781 cgctgaccgt gctgctgtgg gtgctgccag tagtggctat tggcattggc ggttgggtca
    24841 tttacgcccg ctcgcggcgt cgggtacgcg tggtgccgga agcgtttcct gaacaaagcg
    24901 tgccggaagg taagcgtgcc ggatatgtcg cttatttgcc gggcattgtg gtggcgttaa
    24961 ttgtggctgg cgtcagctac taccagaccg gaaattatca acaggtgaaa atctggcagc
    25021 aggccacggc acaggctccg gcgttgctgg acagggcgct ggatccgaaa gccgatccgc
    25081 tcaacgaaga agagatgtcg cgtcttgcgc tggggatgcg tactcaactg caaaaaaatc
    25141 cgggagatat agaaggctgg attatgttgg gccgcgttgg catggcgctg ggtaacgcta
    25201 gtatcgccac cgatgcatac gctactgcgt atcgcctcga tccgaaaaac agcgatgccg
    25261 cactgggtta tgctgaagcg ttgacacgtt catctgatcc caacgacaac cgcctcggcg
    25321 gcgaactgct gcgccagttg gtgagaagcg accacagcaa tatccgtgtg ttaagcatgt
    25381 atgcgtttaa tgcctttgag cagcagcgat ttggcgaagc cgttgccgcg tgggagatga
    25441 tgttgaaact cttacctgcc aacgatactc gccgtgcggt gattgaacgt agtatcgcgc
    25501 aggcgatgca acatttgtcg ccgcaggaga gtaaataaaa agaggcccga tggtgatgcc
    25561 atcgggctat ttttattgtg ccccgcgttg ttgcaggaac agaatggtgg ctgccacgcg
    25621 tgagcggaca ttgagtttgc gcagcagatt gcgaatatgt acttttactg tctgctctga
    25681 aatattcaac accgaggcaa tctgtttatt tgacagcccc tgtgccagct cgtgcagaac
    25741 atccagctcg cgctccgtca gcacgctgaa gggatcttct tccgcgccaa acatttcacg
    25801 ttcacgtaag tactgattga cgcgttcgct aaagactttg ctgcctttcg ctccagcacg
    25861 aattgcttcc agcaatactt ccgggtcgct gtctttcaac agataaccgt ctgcgcctgc
    25921 gtctatcagt gcaaagacat cgctggaggc atctgatacg gtcaggataa taatttgcgc
    25981 ggtaacgcca tccctgcgca aggcattgag ggtatccagg ccactcatac ctttcatatt
    26041 gagatccagc aagatcacgt cgatatccag tctattcgcc agatcgatcg cgctcgcgcc
    26101 gtcgcccgct tcggcgacca cttcaaagcc aggatcaagc tccagtaact gacgaacacc
    26161 gcgtcgcata agtggatgat catccacaat catcacctga aaaggcgttg cttcaggcat
    26221 agtagtctcc tgaggtttta ttagaatcat tatgtttgag cgtagcgttg gtaacgctac
    26281 caggcgcgtg aatgttaccc cattttatgt atggacatgt agtgaaaact acaaccttgt
    26341 cgttcgcttt ggcatcattt tcattataaa gacggaattt tgcatcagct tctcatcaag
    26401 aaaaacaact aaaaaatcaa tcacaatact tgtgcatttc tttaaacagt ttttaaaaac
    26461 tgactccggg tatggagcta tgggtatttt ccgtacccaa tgcttttaac agcaattaat
    26521 ttcataggat gaaagctcaa ttcatcaatc tggttctgtt tctctttgtc gttccgcaat
    26581 atctgttctg gtggctacag cgttatattc acccatagca ttggcatcaa cagttgagta
    26641 tggtgagaca gttgatggtg ttgtcctgga aaaagatatc cagctggttt atgggaccgc
    26701 caataatacg aaaatcaatc ctggcggaga acagcatata aaagaatttg gtgtaagtag
    26761 taatactgaa attaaaggag ggtatcagta cattgaaatg aatggtaccg cagaatactc
    26821 agtattaaat gacggttatc aaattgttca aatgggtggc gcggcaaacc agactaccct
    26881 caataatggt gtgctacagg tttatggcgc agcgaatgat cccacgatta aaggcgggcg
    26941 cttaatcgtt gaaaaagatg ggattaccgt ccttgccgct atcgaaaagg gaggattact
    27001 ggaggttaaa gaggggggat tagcgattgc ggtagatcag aaagcaggcg gtgctattaa
    27061 agcaagcacg cgggtcatgg aggcattcgg aacaaaccgt ctcggtcagt tcgaaatcaa
    27121 gaatggtatt gctaacaata tgctgttgga aaacggcgga agtttgcgag ttgaagaaaa
    27181 tgacttcgct tataatacta ctgtagatag tggcggctta ctggaggtta tggatggcgg
    27241 gactgtaact ggcgttgata aaaaagcagg cggaaaatta attgtctcaa cgaatgcgct
    27301 ggaagtgagt ggtacaaaca gtaaaggcca atttagtata aaagatggtg tgtcaaaaaa
    27361 ttatgaactg gatgatggtt ccgggctcat tgttatggag gacacgcagg ccattgatac
    27421 tatccttgat gagcatgcca ctatgcaatc gctgggaaag gatactggta cgaaagtgca
    27481 ggcaaatgcg gtatatgatc tcggtcgatc agatcagaat ggaagtatca cgtattcctc
    27541 aaaagccatt tctgaaaata tggttatcaa caatggccgc gctaacgtct gggctggcac
    27601 aatggttaac gtttcagtca gagggaatga tggcattctt gaggtcatga agccgcaaat
    27661 aaattatgca cccgcaatgt tggtgggtaa ggtagtggtt tctgagggcg cttcttttag
    27721 aacgcatggt gccgtggata ccagcaaagc ggacgtttcg ctcgaaaata gcgtatggac
    27781 catcattgcc gatatcacta cgacgaacca aaacaccctc ctcaacttag ccaaccttgc
    27841 gatgtctgac gcaaatgtga ttatgatgga tgagccagtg actcgttcat cagtgacggc
    27901 aagtgcggaa aatttcatta cgttgaccac caataccctg tcgggaaacg gcaattttta
    27961 tatgcgtacc gatatggcta atcatcagag cgatcagctc aacgtcaccg gtcaggcaac
    28021 aggtgatttc aaaatattcg tgacggacac cggtgccagc ccggcagcag gagatagcct
    28081 tacactggta acaacgggcg gcggtgatgc tgcatttacg ttgggcaatg ccggaggcgt
    28141 tgttgatatc ggtacgtatg aatatacctt gctggataat ggcaaccata gctggagttt
    28201 ggcagagaat cgcgcgcaaa ttaccccttc aaccactgat gtgctgaata tggcggcagc
    28261 acaaccgctg gtatttgatg cagaactgga caccgtgcgt gagcgtcttg gtagcgtaaa
    28321 aggcgttaat tacgatacgg cgatgtggag ttcggcaatt aacacccgca acaacgtgac
    28381 cactgatgcg ggcgctggct ttgagcaaac attgaccggg ctgacgctcg gtatcgatag
    28441 ccgtttctcc cgtgaagaaa ccagcacaat tcgcggcttg ttctttggtt actctcattc
    28501 tgatattggt tttgatcgcg gcggcaaagg caatgtcgat agctataccc tgggggctta
    28561 tgccggttgg gagcatcaga acggtgccta tgttgatgga gtggtgaaag ttgaccgttt
    28621 tgccaacacc atccattgca agatgagtaa tggggcaaca gcgtttggcg attacaatag
    28681 taacggcgcg ggtgctcatg tcgagagcgg gttccgttgg gttgacggat tgtggagtgt
    28741 tagaccctat ctggccttta ccggctttac cacagatggt caggactaca cgttatcaaa
    28801 cggcatgcgc gcggatgtgg gaaatacccg gatattacgc gctgaagcgg gaacggcggt
    28861 aagctatcac atggacctgc aaaacggtac gacgctggaa ccctgactga aagccgccgt
    28921 gcgtcaggaa tacgccgatt ctaaccaggt gaaagttaat gacgatggca aatttaataa
    28981 tgatgtggct ggaacccgtg gcgtttatca ggctgggata aggtcatcgt ttaccccgac
    29041 gttaagcggt catttgtcag tcagctatgg caatggcgca ggggtagaat cgccgtggaa
    29101 tactcaggcg ggtgtggtct ggacgttctg ataacaaaaa ataaacaggc tgtgatgtgt
    29161 cacggtctgt ttatcgaatt aattgcagat ataaaaaaaa ccaaccgtaa aggttggttt
    29221 tttcttggga tttttggtcg gcacgagagg atttgaacct ccgacccccg acaccccatg
    29281 acggtgcgct accaggctgc gctacgtgcc gactcgtggc tgctaatact accgttttcc
    29341 acaccgattg caagtaagat attttgctaa ctgatttata attaatcagt tagcgataaa
    29401 acgcttctcg tctgtcagca cttgcaataa caaacttaac tgtggttttt catctttcac
    29461 tctttcacca cgtaagttgt aagtgcggta tttaccgtta ttgttcagca ccagcgtctt
    29521 tttcggggtg gtaattgcca gcgtatcgtt atccgctgca gtaacccagt aatgacggcg
    29581 ttgagggttg aacaaatcct gaccttgcga atattcgctg gcaggtgtgc tgacatgtag
    29641 caggcgttgc atcagcgtcg tcatcagatc ggtatgatca gtcagagcat taatacgctg
    29701 cgccggcgtg cctggccagt gaatcactaa tggcacctgc agatgaccgt gggaccagtc
    29761 aaaggtttct tcgtcttcgc tcagcggaat accccgaccg gcagtgataa tcaccaccgt
    29821 attgtccagt ttgccagaat cacgcagtgc attgagcacg cggttgatct ggtcatcgac
    29881 attgcctgcc gcccggctat atttccgtgc aaatgcctgc tgattgctgt cgtcaatgtt
    29941 agtgccattg aaagagaccc acgagaacca gcggttatct tcttgtgcgt agcggcccag
    30001 ccagttgatc cactgcgtgg cggtctgctc gtcggattgg gtgcgtacgc tcggcatcga
    30061 gaaatctgac aacaatgcct ggcgatacag cgggctggta aagccatctg atgagaataa
    30121 tcccagctga tagccttgct gattaagcgc agtaattaac gccgcagggg tacgggtcga
    30181 cagaatgccg tccatatagc tcggcgagat gccatagaac aggccaaaga tgccgttgtc
    30241 tgtagtgttg ccggagctca tatggcgcgt gaacgaaata ttttgctcag caaaacctgc
    30301 cagcgcaggc atctgcttct cgaagcgtga gtagttcagg ccatcgacag taatcaacag
    30361 cacattctgc ccggtgccca tatcgcgata gcgcagttcg cttaacggat actgaacgga
    30421 aacggcgtct ggattacctt gctcaataag acggcgttga tactcctgcg catcaagcag
    30481 accatgcttc tcaagaaaac gtcgcgccgt catcgggtac gaaagcggca ggttagcgcg
    30541 ctgcatggtg atcgggcgat agaagttggc atcggcccag atatacacca catgcgaggc
    30601 gataaaggcg ataaataaga atgcggccag cgggcgcgcg aagcgccgac gacgcgtcag
    30661 gctgcgcagc ttttgccagc tccacgtcgc aaacaccagt tcaagcaata aaataaccgg
    30721 cacgctgatg aacatcagct gccagtcgcg cgccatctca ttttcgtctg ggttgataac
    30781 cagttgccag acgatgggat taagatggag atggaaacga gtaaagactt cgctatcgat
    30841 cagtaatagc gtcattcccg ccgttgccag aatgacggac aaaaacctca tcagcctctg
    30901 ggagccgacg ataaaggtca gcgggaagag gatcagcaag taggtggcga acaccaggaa
    30961 gctgaaatgg ccgataatgc ttacgtagga ataaatgcga ccagcaagcg ttgtcggcca
    31021 gtcggcgata aacaggtaac ggctgccaat gacgagcgaa agcagaatat tgaacagtgc
    31081 aaaccagtgc ccccaactga ccatctggga gactttttca cggtagcgct gacgatgagt
    31141 taccataacc tgttatctgt ttcccttagt gcgctttgtc ttcgttgata gaggactgta
    31201 aggcgcgggc aaaagagttg gcaattgcct ggcgttgggc cggggcaatg ctggtattga
    31261 taaggttagt gaccatgttg cctaacacca tcagggaaag gtcggtcgga gccttatgtt
    31321 tttccagtac gttgagcagc tctgcgagca attgttcaac ctgttcatca ctgtagcggg
    31381 aaatttgtgg cataaatcga aatcagttag ttgatgaaag ggcaacatat taccgtagca
    31441 acggtttttt ttctgctttt ttatgcgtta aatcattctc gttacgctgc cttctgtaaa
    31501 atgaccgcca tcttttattg cgtcgcttaa ccggcggtgg ttgaataccg cccggtctta
    31561 aaggagagtt tatcatgagt ctggatatca accagattgc tctgcaccag cttatcaagc
    31621 gtgatgagca gaaccttgag ctggtgttgc gcgattcatt gctggaaccg acagaaaccg
    31681 tcgttgagat ggtggcagaa ctgcatcggg tctatagcgc caaaaataaa gcttacggct
    31741 tgtttagcga agagagtgaa ctggcgcaga cgctgcgctt acagcgtcag ggcgaagagg
    31801 actttctggc atttagccgc gcggcaaccg ggcgcctgcg tgacgaatta gcgaaatatc
    31861 cttttgctga cggtggattc gtgctgtttt gccactaccg ttatctggcg gtggagtatc
    31921 tgctggtggc agttctgagc aacctgagca gtatgcgcgt taacgaaaat ctcgacatca
    31981 atccaaccca ttatcttgat atcaatcatg cggatattgt tgcgcgtatc gatttaaccg
    32041 aatgggaaac caatccagag tccacccgct atctcacttt cctgaaaggg cgagtagggc
    32101 gcaaagtggc ggatttcttt atggatttcc tcggggccag cgaagggtta aacgcgaaag
    32161 cgcaaaaccg tgggctgttg caagcggttg atgatttcac tgccgaagca caactggata
    32221 aagcagagcg acagaacgtg cgccagcagg tttatagcta ctgtaatgag cagctgcagg
    32281 caggcgaaga gatcgaactg gagtcgctgt cgaaagagct ggcaggcgtc agcgaagtga
    32341 gtttcacgga gtttgcggct gaaaaaggct acgaactgga agagagcttc ccggcagacc
    32401 gtagcacact gcgtcagttg acgaagtttg ccggtagcgg cggtggcttg actattaact
    32461 ttgatgcgat gctgttaggt gaacgcatct tctgggatcc ggcaaccgat accctgacca
    32521 ttaaaggcac gccgccgaat ttgcgtgacc aattgcagcg ccggacgtct ggcggcaatt
    32581 aatcggtttt gtgttctgaa tgccagatgc ggcgtgaacg ccttatctgg cctgcaaaaa
    32641 atagcaatac cgtaatcagg cataatctgc agatacaaaa aaccccgccg gagcggggtt
    32701 ttttacaact tattcagcaa ttaagcgcga acgaagtcga tgtgctgcag cttcggtttg
    32761 tacgggtgac gctgtacgtc ctgagcttta actttgattt ctttaccgtc aacaacgatg
    32821 gtcagaactt cgctgtagaa ttcagcttta gcttgcatgt tcatgacttt gtcgtgatcc
    32881 agctcgatag ccagcggcgc ttctttgcca ccgtagatga ttgccgggaa cttgttagcg
    32941 gcacgcaggc ggcggctcgc acccttaccc tgctctttac gtacttctgc gttgatagta
    33001 aacatttctt tctctcttaa attaatcctg ctacaggcga cccagcaaca ggtaagtgat
    33061 ctgctttgcg tatgcaaaag cgggcgagat tctatactca atcgctttat acatcaatca
    33121 aaactaccat taaccgcgta attcgtgcgc ccgacgaaaa cgaccttcgt aatcgaacac
    33181 tttttcacgc acttgccagt actgaccttt catgcgggca acgacaaaat ccgggtgtcg
    33241 caataaggct tgctgggcga ggatatcggc ggcggtgatc cagcgcagag ggatgcccgg
    33301 tgtgcgcgta tgcgggcgga taaaaagctg ctcgaaggca gttcgctggg cgggcgtttg
    33361 cagacggaaa cgctcactca catccgcgct gtcttcatcg taataggtga ttttcaacca
    33421 ttcgcctttc tcgtcgtgct catgctgcaa agacatgccg ctacagcgta ataccagcgc
    33481 atctttcagt cgtagcgccg cttttaacat atcatccgga tcaaccagta ccgtgtcaca
    33541 ttcgcggcag cggcgagcgg caatatcatt ttctgcgtta cattgcgggc aatttttaaa
    33601 acggaaacgg aagtcacact gttcgcgatg accgtcgtca tcttcaaacc atccctgaca
    33661 gcggcgacca aagtgttcaa tcaatgtccc gtcggcggtc gttttccccc aaaaggtgtt
    33721 ggcaaaaccg caggcagggc agaaaacctg taccggaacg ttgtcacttt tgcctttcgg
    33781 tgtaccaact tccggcgcgt agagatcgtg aggattaccc gcataatcaa ggattaaaca
    33841 atcagtcttg cccggagcga gacgcagacc gcgcccgaca atttgttggt aaagactcac
    33901 tgattcggta gggcgcagaa tggcgataag atcgacgtgc ggggcgtcaa atccggtggt
    33961 cagtactgcg acgttgacca gatagcgaaa acgctgggct ttaaaatctt caattaacac
    34021 atcgcgctca gcgccggggg tgtcgccagt aatcagtgct gcatcttcgg caggcagtaa
    34081 tcccacaatc tcttttgcgt gttcaaccgt cgcggcaaaa atcatcaccc ctttgcgcgt
    34141 tgcagcaaac tccataatct ggctgatgat gtgcggggta atacgttgtt gttttttcag
    34201 ctcacggttg agatcggctt cgctgaacag cccgttactc tgtgcctgca agcggctgaa
    34261 atcgtattgc actactggca tatccagtcg ttctggcggc gtcagatagc cgtgtttaat
    34321 catataacgc agcggcagct cataaatgca gtcacggaaa agggctttct catcgccgcg
    34381 taccatgccg tgataatgaa actggtagat ccagcctttg cccaatcgaa aaggcgtggc
    34441 agtcagcccc agcaggcgta agtggggatt cacttttgtc aggtgagtga ggatttgctg
    34501 atactggctc tcttcatcgt caccaatacg gtgacattca tcgacaatca acagcgaaaa
    34561 ttcaccctga aaggcatcaa gattacgggc gacagactgc acgctgccaa ataccacttt
    34621 accgtggctc tctttgcgct ttagcccggc ggcaaaaata tcggcttcca gccccagcgc
    34681 ctgatacttt gcatggtttt gcgccaccag ttctttaacg tgtgccagca ccagtacgcg
    34741 accacgagcc agccgtgcca gttccgctat caccaggctt ttacctgcgc cggtgggcag
    34801 cacgataacg gcaggggttt tatgacgacg aaaatggttg agcgtggcat ccacggcttc
    34861 ttgctgatat gggcggagtg taaaaatcat ggtctcgcgt aggtagatta ttctgctaat
    34921 agtatgccac ggttcttttt cccttgaggg aagttattag accgctatac tgggcagaca
    34981 acagttcaga ttttcgggtc attacccgac ctccgttttt tacaggcaaa atcacacaca
    35041 tgcgacttga taaatttatc gcacagcaac tcggcgttag ccgtgctatt gccgggcgtg
    35101 aaatccgcgg caatcgtgtc accgtcgatg gcgaaatcgt ccgtaatgca gcgttcaaac
    35161 tgctccctga acatgatgtc gcttacgatg gcaacccgct ggcgcagcaa cacggtccac
    35221 gttacttcat gctcaataag cctcagggct atgtttgctc cacggacgac cctgatcacc
    35281 caacggtgct ctattttctt gatgaaccgg tagcgtggaa actgcatgcg gcggggcggt
    35341 tggatattga taccaccggt ctggtgctga tgactgatga tggtcagtgg tcgcaccgca
    35401 ttacttctcc gcgccatcat tgcgagaaga cctatctggt gacactggaa tcacctgtag
    35461 ctgacgatac ggcagagcaa tttgctaaag gcgtgcagct gcataacgaa aaagatctca
    35521 ctaagcctgc ggtgctggaa gtgattaccc caacgcaggt tcgtctgacc atcagcgaag
    35581 ggcgttatca tcaggtgaaa cgcatgttcg ccgccgtggg taaccacgtg gttgagctgc
    35641 atcgtgaacg tattggcggt attacgctgg atgctgattt agcccccggt gaatatcgtc
    35701 cgttaactga agaagaaatt gccagcgtcg tctaactttt caatgacttt caggagcccg
    35761 ttgtgaccac ccgacagcat tcgtcgtttg ctattgtttt tatccttggc ctgctggcca
    35821 tgttgatgcc gctgtcgatt gatatgtatc tgcccgcgct accggtaatt tcagcgcagt
    35881 ttggcgtacc ggcgggcagt acgcagatga ccctcagtac ttatattctg ggctttgcgt
    35941 tggggcagtt aatctacggg ccgatggcag acagcttcgg gcgtaagccg gtggtgctcg
    36001 gcggtacgct ggtgtttgcc gccgccgcgg tggcgtgtgc gttggcaaac accatcgatc
    36061 agctgattgt gatgcgtttc ttccacgggc tggctgcggc tgcggccagc gtggtcatta
    36121 acgccctgat gcgcgatatt tacccgaaag aagagttctc gcggatgatg tcgtttgtca
    36181 tgctggtgac cactattgca ccgttgatgg caccgattgt cggcggttgg gtgctggtgt
    36241 ggctgagctg gcattacatc ttctggatcc tggcattagc ggcgattctg gcttcggcaa
    36301 tgattttctt cctgattaaa gaaaccttac caccggagcg tcgtcagcca tttcacattc
    36361 gtaccactat tggtaacttt gcggcgctgt tccgccataa acgtgtcctg agctacatgc
    36421 ttgccagtgg tttcagcttt gccgggatgt tctcattctt aagcgccgga ccgtttgttt
    36481 atattgaaat taaccacgtc gcgccggaaa actttggtta ttactttgcg ctaaacattg
    36541 tttttctgtt cgtgatgacc atctttaaca gccacttcgt ccgccgcatt ggcgcgttaa
    36601 atatgttccg ctcggggttg tggatacaat ttattatggc agcgtggatg gtcatcagtg
    36661 cgctgctggg gctgggattc tggtcgctgg tggttggcgt tgcggcgttt gtgggttgcg
    36721 tgtcgatggt gtcatccaat gcgatggcgg tcattcttga tgagtttccc catatggcgg
    36781 gaacggcatc ttcgctggca ggaaccttcc gttttggcat aggggcaatt gttggcgcat
    36841 tgctttctct tgcgaccttt aactctgcat ggccgatgat ttggtcaatt gcattctgcg
    36901 caaccagctc cattctcttc tgtctgtacg ccagtcggcc gaaaaaacgg tgatctattg
    36961 cacaacgagg aaggtaaaag gcttcctttg ttgatgcatg tcaaccacaa atct
//
LOCUS       DAZGSP010000046        36412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZGSP010000046 DAZGSP010000000
VERSION     DAZGSP010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.046"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..355
                     /locus_tag="ACHK74_002475"
     CDS             <1..355
                     /locus_tag="ACHK74_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HFP0221281.1"
                     /translation="AASAGTTIYTDSHQRPMNPPAGVRVVLLDAPEQTQDTFFGVLPA
                     EPAEASARVMERMQSPEWQSFQAGLAEHYRALAHAWSLGLKKYPAVVFDDSEVVYGTT
                     DVVLAEQLRTGGGQP"
     gene            352..1326
                     /locus_tag="ACHK74_002476"
     CDS             352..1326
                     /locus_tag="ACHK74_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFP0221282.1"
                     /translation="MISCTSRHRAGVLLLSMAVLPVAEASLNTAQIVASAASQNCISW
                     RVSGICYWLFCTKFSCKVRTSVKVSHYIPEVVVSTYTAPGGNPWREMSAVSSTAGGPE
                     NAISKGIAGLSAGGGNPADMKVQGERRSSIRFKYADATGHPATTLIGGMVPGYSCESP
                     VTPFVPYFLSTLDSIAWRTGLPESLYPEALIPGKREIGSQEEENMWGNVYPRSGFVTQ
                     TDDDKAAAVVAQRVADIITRTGEPHVYQPVKAGKKDGYWPPDPVEENTGTRNHKWQRL
                     TPSVSTSCAVFPDGEHEAAETGNAAFALWQPYSCCKKRGQTFLGSTNF"
     gene            1340..2761
                     /locus_tag="ACHK74_002477"
     CDS             1340..2761
                     /locus_tag="ACHK74_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFP0221283.1"
                     /translation="MKQKMTILVLAVVLCAGTVQADGNFLSLPKVNDSAFGYGKSVDG
                     AVSDKLFYTLGGGSVISQPATRSNMQKLGVDLGWSSDLMCGNFDLKTTVGNQLNGITD
                     GFKNLMGDVIQGASGAVASLPAMVIQRANPGLYDMLTNGVLQANVAFDKAQLNCQNMA
                     KRMMEFTDSNKWTQAAMLEEYKKIVNTGDGDAIKGDEAGRQVTGEEGQRWIGGQQRGG
                     AGQSAIRPTHDLTAAGFNMMNSLPSTSTSSVSPGNCNGSACMKYRNAEEAARAVVKVL
                     GDKSMRTCGDAKACTSGDADQQPGTSIAGTGFAPMLEEATRVNTELLAGMVNGRDKPT
                     AGNLAKLKAGSLSVTAGVIRALQRDPDNGALTSRLAGELAMADTVETALLMRRMLLTG
                     MAEPYAAAQPAALEEGERRIEALDREIMALKNEMELKRDLARNSVLTIIERDNERVGN
                     NPMIQQADNADSRIHSLDAPDKE"
     gene            2766..3107
                     /locus_tag="ACHK74_002478"
     CDS             2766..3107
                     /locus_tag="ACHK74_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221284.1"
                     /translation="MSRIYGRVKKRMKVFALYTLLLTGITVFSLWLADTGIRHAEGFA
                     ALREWMYETRYGWLGWRLCLYSAIGWLGWRIWHAPGFRAEYRASFLRMATVSVLFILL
                     CEYAIFAEPGR"
     gene            3114..4619
                     /locus_tag="ACHK74_002479"
     CDS             3114..4619
                     /locus_tag="ACHK74_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFP0221285.1"
                     /translation="MTTNNYLEYFLTLLGWVVNNGLWNVLISTGLFALPLAFRIIGIW
                     LKVREEGADEGNKGMLSLPRIEHVLYGAFFVMISCCVPLVQVTLDTLSFDKSRAQTCG
                     VWTPEEPGETGYSGVISSIGDQTAAAPVWWVVVHKLSKGVTQAAVASIPCRPDLRQLR
                     FEIQRTFIENRALADELQDFTNDCYSLALYMWKQRDQGQSKDKKVLDDISWIGSATFM
                     QGDYGTLQSKMPRAAFPWNTARDSGRPDTGRRGYPTCREWWSDSSVGLKKRVMEEVSD
                     HYWLRLSAAMKMIGSSQVDYKEEVIKRLVSPANLTLSQGGEVYRGYGGNADMTTMNAV
                     ARIAGTAGSAVAALGAFPALDAMRQALPMVQSIILMAIYIMIPLILAFAAYELSTLIT
                     LTFVVFAVHFLTFWWELARWLDSWMLTALYSSDTHTRFNMMGFQNTSDDLIMNLVMGT
                     MFLVLPAVWLGALSWAGVHIGDGISRGLPNGISEAKGAASSAGSIANRGIK"
     gene            complement(4667..4909)
                     /locus_tag="ACHK74_002480"
     CDS             complement(4667..4909)
                     /locus_tag="ACHK74_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221286.1"
                     /translation="MNNESEKAMWRCKPVDNPWTLDSLLKKTISAVLPASDKVSHQNK
                     NKLRNNEFIKIEHPCSKFESRIDGDYIISGGTEYRL"
     gene            5489..6019
                     /locus_tag="ACHK74_002481"
     CDS             5489..6019
                     /locus_tag="ACHK74_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005285503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221287.1"
                     /translation="MKNANRVVINLFSISLVTMISGCASYWGKVPYNPPSDTEKNIAH
                     VRLIGNPPGFTISQYGNKPAAVDPSNMVILRPTHDLGFPKVNNDPERYKETYYDTNVY
                     AGTPTRIGFKFLHGCEDVGYTFLPEKGETYEFRLSVTDKTGYCVLYAFHLKYDSENKL
                     YITENIEEYKGYVYRE"
     gene            6021..6359
                     /gene="bamE"
                     /locus_tag="ACHK74_002482"
     CDS             6021..6359
                     /gene="bamE"
                     /locus_tag="ACHK74_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005285504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFP0221288.1"
                     /translation="MCVYLSGGFLLCLLVGCIGHIDRTNETTLGKYSKQEIFNKLRKQ
                     KTTKREVLSLLGRPNYPYDYNDKNTWIYLSKTEGKVLYGAIPVDFNRSVMLKLVFGKD
                     KTIEGIEYKQ"
     gene            6511..6828
                     /locus_tag="ACHK74_002483"
                     /pseudo
     CDS             6511..6828
                     /locus_tag="ACHK74_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(7214..7474)
                     /locus_tag="ACHK74_002484"
     CDS             complement(7214..7474)
                     /locus_tag="ACHK74_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221289.1"
                     /translation="MSKKPSVYLLLLTIIYCWVIFFLIGSVVLLIVNFWEGGCFYFSE
                     KQFKAAITLSGIAGGGAGLRSWIFAWIDERKARKSPPSVPKA"
     gene            complement(7467..16394)
                     /locus_tag="ACHK74_002485"
     CDS             complement(7467..16394)
                     /locus_tag="ACHK74_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFP0221290.1"
                     /translation="MNRNCYRIIFNKARGMLMVVADIARSGRAGASPSSRTGYPRRQR
                     ICRVTPLAFSLWLASGMVHSVSAAGIVADHGAPGHQQPTIMQTASGIPQVNIQTPSAG
                     GVSHNTYSQFDVGNQGVILNNAHNNVQTQLGGMVAGNPWLAKGEARIILNEVNSRNPS
                     QLNGFVEVAGKKAQVVIANPAGISCDGCGFINANRATLTTGQPQMKNGSLTGFRVEGG
                     EIQVTGKGMDASRTDYTDIIARSVKINAGVWAQDLKVTTGRNNVDIAHGQTEKKAADA
                     SSQPQVALDVSSLGGMYAGKIRLVGTETGVGVRNAGHIGAQAGAVTLTADGRIENSGS
                     ISAKTDVHLATTRELNNSGSVYAGQDTQIQSDGVFTHTGSVASRRNTRIQTTRLTGGE
                     RSLLAAGVKDDGRLAEAGNLTVSTTGELAAHGQVLSGGDMQLKGQGLDLSNSRIQGQH
                     TGLDAGSGNLSTQNAQLSAGTFAARTAGQFSNNGATINAGTLQISAQSLSNHKGSLIQ
                     TGTGDFSLSLPGSVDNREGLLAANGAVRLDALSLDNRRGKVQAAQSGSLQVKTTGAVD
                     NLQGTLIASRDILINTQALNNDNGLISAAAGTGRIKTQQAVSNTAGRMESAGRLDISA
                     GNLNNHQGTVVSDELSVTLDGALDNTSGRLLSQKTLSVSGSELVSDDGLIQSGSDMTL
                     DVQDGVLSNRNTKTRGGISSAGTLTVRAGMLNNQQGFMAGQKDVTLNTGTLDNRQGAL
                     GSQASLQVSSGTLMNQKGALKAGTDMLLSGDDVSNQEGTLAAGGDLNAHLNVLENQQG
                     TVVSNGNSRLDVTRSDNQGGRLVAQQSLTLTSKDIINDAGGLIQSGASLNIRADTLSN
                     RDSGDRGGVISQGPVTLNSGTLDSTAGVLLSGDALSLTAGVVNNTSGQVVANGLLGWN
                     SQALNNQSGLIQGWGISINTAGQTLDNRRGTLNSLQELTVSSGAMDNRGGTVGAKTTA
                     DLSTTSLDNREGGRLVSEGEIRLHTRGLQNSHGQIQSVGDMLLDSVRGVVDNVSGLIR
                     SGSAITLNALQFINRHTQNTGQGLEAQTIHITTQDLDNQEGSILADRALTVMADRTLS
                     NNDGVLSSGATLSVSGRQLAFSNRDGVVKAGQSVSVDAGQLGGDGKLLSPGDMTLKSN
                     TAFSNSGQTIANGNLTLSVNGDVTNTGSLLAGSRLELNSICLENTEKGEISAGQTWLN
                     VTDTLLNRGLIDGKYTHLQANTLTNSGTGRIYGDAVGASAATFNNLDENGVAATLAGR
                     ERVDLGVQTLNNRTHSLIYSAGDMHIGGTLDANGAATGKAGVLNNHSATIEAAGYLAL
                     SAGQINNVNDHFTTERVVVSTEKVTEYQLRGSDKRWSAGEPGVYVDNDSSNSLKKLHT
                     PEGARDKFTQYDYTRTVEETRVKDSDPGKILSGAGMTIVADKLLNDKSQVVAGGLLTI
                     PSGSVENVSVSGERHVTDSGTSTYYYRIRKKGKDKQGEKTSQYTPPTVIQSITLKPGE
                     LTSHGQIQGSQVTLSPLKPQGTDVQTGQAGNVDAAVAATDRIPLRPVVSAGEPVILLP
                     GQQFEVSTPQGSIHVAGPDTRLPDSSLFKTNPAVNVPYLVETDPRFTNQKMWLGSDYM
                     QKAFSQNGDNMLKRLGDGFYEQRLIREQVVALTGQRYLDGYGSDEEQFKALMDAGIAF
                     GKQYNLKPGVALTAEQMALLTGDIVWLVNTTVTLPDGSTQTVQVPQVYARVKPGDVNS
                     AGALIAGRDMVMKLDGDLFNSGKLAGKQTVQLSAENIHNQAGSIQGANVSLTARTDIN
                     STGGLLQATDSLLAMAGRDISLTTTTRTAQSDAGQNHFERTSIDSVAGVYVQNDQGRL
                     VLQAGRDMNLTAATVVNQGKDSLTQLSAGRDMTLSTVTTSAQDNITWDKNNRLSQGVT
                     QSTGSTLAGNGDVTLTAGRDMTSQAASLSAQKGLALIAGHDVTLTGAQNTSSLDEYHK
                     VTGSSGMLSKTTTTTHDITDRRTMTGSELNGDTVSISAGHNLNVTGSSVAGDNRVSLA
                     AGNDLSIGTLTESNRETHLKQEKKSGLMSSGGVGFSVGSQSLKVTDTATDTTQKGSTV
                     GSVHGDVSLQAGNRLSANSADLIAGRDMALSGKEVSISAATDQHVQTHTVEQKTSGLT
                     LALSGTAGSALNTTVETVQAAKSAGNSRLEALQGVKAALSGAQAVQAGRLADAQGADA
                     GNNNTVGISLSYGSQSSKSEQQSEQTVAKGSTLTAGNHLSIQATGSGVKGVDGDLTIQ
                     GSQIKAGNNVLLQANRDVNLVSAENTSKLEGKNTSGGGSVGVGIGVGAGGWGISVSAS
                     ANQGKGSEKGNGTTHTETTVDAGNRLTIISGRDTTLTGAQAGGETVKVDAGRHLTLTS
                     EQDSDRYDSKQQNASAGGSFTFGSMSGSASVSLSRDKMHSNYDSVQEQTGIFAGRGGF
                     DVTTGQHTQLNGAVIASTATADKNRLDTGTLGFSDLENRADFKTEHQSAGLSTGGSVA
                     GNFLGNMANNLLVGANHEGHADSTTQSAVSAGNITIRDTKSQKQDVADLNRDAAHANQ
                     TLSPIFDREKEQQRLQMAQLIGEIGNQVADIVRTEGQIAGEKAKRDPAALNQARAELE
                     AAGKPFTEQDVVQRAYNNGMAASGFGTGGKYQQAIQAATAAVQGLAGGNLSAALAGGA
                     APYIAEIIKQTTPDGAGRVAAHAVVNAALSLAQGKNALAGAAGAATGEVVGMIATQMY
                     GQPVSELSETEKQTVSTLATVAAGLAGGLVGDSGASAVAGAQSGKTTVENNALSLPSG
                     MVSYGQAVASWNQYADANNLTPEQKQAGLDKLAKGELPEGANISKVIVDGYKDGVLIA
                     GAWYLGPAVSVGKAAVGGVTSAAVNGGFQWYGLSQPGSENQSWDYKGSASSFMTGMLA
                     PGRTVTANTAIALGGSVFSDGANIKSQSGTALGAYAGGYVGEYFPVILRPVLGNSAST
                     VGDIGGGFVSELINYGIKNYPTPTNKEPEHE"
     gene            complement(16410..16937)
                     /locus_tag="ACHK74_002486"
     CDS             complement(16410..16937)
                     /locus_tag="ACHK74_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFP0221291.1"
                     /translation="MRNGKYDVLSPLYSGEPVNEAEVLGAVVWLWMHSPLHRDAPLHT
                     LPDLLLPVIKNRQYVVATEQGRPVFFMSQAWLSPEAEARYLTQPAILMPQSDWNSGDR
                     MWVCDWVAPFGHTPDMSRLVRQDIFPRLCWRSLYHRGQQTGLRVMNFRGAQVSREEAR
                     QWRVQHPLAVDVPER"
     gene            complement(16947..18026)
                     /locus_tag="ACHK74_002487"
     CDS             complement(16947..18026)
                     /locus_tag="ACHK74_002487"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFP0221292.1"
                     /translation="MEIVPGEQPGESDILITRKQDKYWRLGFSLDDSGTRSTGRYLGG
                     ITFSLDNPFSLSDLLYVSATHNFPHYGGKGSKNYTAHYSVPFGYWQFSVTASDYDYHQ
                     TVAGAVEDYQYSGESQNLGMQLSRVLHRNGTQKTVLTYDVQARRSRNYINDTEIQVQR
                     RQTAAWRLGLQHRHYISQATLDAGVSWQRGTRWSGAQPAPEETFGEATALSKILHINA
                     SFNLPFTLAEQPFSYSFRYQRQLSNTPLTPQEQFAIGNRWTVRGFDGERTLNASNGWY
                     IRNDLAWRTPLPEQALYLGMDYGEVGGAGSEYLVGRHLAGGVVGLRGRLLSTDYDVFI
                     GRPFSKPAGFMTSDVTAGFNLFLNF"
     gene            complement(18024..18599)
                     /locus_tag="ACHK74_002488"
                     /pseudo
     CDS             complement(18024..18599)
                     /locus_tag="ACHK74_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
     gene            19314..19886
                     /locus_tag="ACHK74_002489"
     CDS             19314..19886
                     /locus_tag="ACHK74_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221293.1"
                     /translation="MLTKKMLLVCASCVALSACSAQEIAELNRQISDGAFAATKVLNV
                     GVNQADDDLKYLSQAPRAKREKRDHVIEVPVDVDTAAGRLKRYYKFYSNEEVDAIRNN
                     GHANGKWAAAAISQNGHMWDAMPGSYYKMGSDWDGGEFDDHLTLELEKNGKGSRIYIT
                     YASSAPGHLKDKFIEPLVKRVKDVAEGRAR"
     gene            20280..20579
                     /locus_tag="ACHK74_002490"
     CDS             20280..20579
                     /locus_tag="ACHK74_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HFP0221294.1"
                     /translation="MNKMFSQWIDFDEMITPKIIKVIYWFFLFITVAGGVIGMIANPF
                     VGLLGLIMGIIGLRIGCEMIILLFNIYKQVKKIADAVPDKINEQAEDSLLSQNKH"
     gene            21678..22580
                     /locus_tag="ACHK74_002491"
     CDS             21678..22580
                     /locus_tag="ACHK74_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFP0221295.1"
                     /translation="MSRREKLLKQDLVAHIRKDKGSFKKLSDYTRIMHRFAESLAKMN
                     VQISSAAQVKVRHIELYMRSRSNVSGRTRENEMSAIRVMLRQAGKYKMADPEHPRLSN
                     KALGISGSSRKGTKKLITDERFREILQRVEAKDKGVAAVVKLSRYLGLRNEEAIQSAK
                     SLRTWKKALQRGDEKIRVIFGTKGGRERMTTVVDRDNVLDAVNYALKYASEHNGRLID
                     RDNLKSAMDYYLNTLRRYGELKGGDETAHSFRYSYAASAMKYHIMNGYSYKEALALTS
                     IDLGHGDGRGRYIEQVYCQLLGYE"
     gene            22613..22936
                     /locus_tag="ACHK74_002492"
     CDS             22613..22936
                     /locus_tag="ACHK74_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221296.1"
                     /translation="MDCTILLKIISVKEITKNSPLWSHYLARYVDSGYQAEFECEGTA
                     DKWDIYDEWDIYESIADYFDVVGDFNCDVRWSGRKILIKGTCVFPLGEPVKGIHNIPL
                     WIDVE"
     gene            23402..23794
                     /locus_tag="ACHK74_002493"
     CDS             23402..23794
                     /locus_tag="ACHK74_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221297.1"
                     /translation="MSTFILYPEPGISSFFRRMTAQLRVFFSRNNLSYYSRWYTNPAG
                     NLSVRVCMFRRGGVWTPCLYYRQGMYRNEYTGWYQPLPREVIYLNRFTGRISRRRTWH
                     AACRMAEQLTTLCYRFGLQGDGIVAQPC"
     gene            23877..24356
                     /locus_tag="ACHK74_002494"
     CDS             23877..24356
                     /locus_tag="ACHK74_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221298.1"
                     /translation="MHSLLILLYVVVWRACIILRVIAPVSAVRAWCEYQLEGMTILRD
                     AYSSLAKAAGMQNDAFEYTESIARHLGYKSGGDQLKSGLFRPFLWHIRVKGHRLGAVM
                     EMEAWKTWKNGCNDVCNPWEDVCNPWERGWNDGWTECLNQRIAGLLKQACIEFSDAD"
     gene            24552..24929
                     /locus_tag="ACHK74_002495"
     CDS             24552..24929
                     /locus_tag="ACHK74_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221299.1"
                     /translation="MYSPLIAHYSALQTQSALLAHISQMEGELMRLRAWQRLGITPEP
                     DDETEPSLVYVYLWDTGEALGWLLYDLEQEEISAPGIQARQALDSLMALGREINHEIW
                     ADSISTGKMVAGADEYFARHDMM"
     gene            25011..25640
                     /locus_tag="ACHK74_002496"
     CDS             25011..25640
                     /locus_tag="ACHK74_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221300.1"
                     /translation="MSELSSRPAREPVVYTLEQVSTIPEKQWHAFVLAATETFWQLPE
                     ALRPQNAYFGSLTRASELFPVTDTLAFYCRSADGLWSVNVTIEREHSRNILALNELNF
                     GRQPGDFFARTVFVLLHNLCPDCFRIHSTVGGASWSLPLKWIKRYLGHENFSAPESVL
                     TTLVRGDVFDSLLLQFLSGQGRQLSPDDWAALEEAEYQLYWLRALAGGR"
     gene            25756..26139
                     /locus_tag="ACHK74_002497"
     CDS             25756..26139
                     /locus_tag="ACHK74_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221301.1"
                     /translation="MGLDIYFLARDNHHSGAVVGTQEHQEVGAFRKVKPLLTWFREHT
                     GASDEQHAFPVTRSSLAALLRDLSMLTPGNCHRLFPYVTGSCLRGEDYAGDYWYDVDD
                     VKAWVSNTLKTFDFERRSLVMLALW"
     gene            26232..27209
                     /locus_tag="ACHK74_002498"
     CDS             26232..27209
                     /locus_tag="ACHK74_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HFP0221302.1"
                     /translation="MKTKKHTSRTAIKTPERDLYQLVTDRVVTAIENGVPPWKRPWRS
                     ARNGVGLPEMMPANALTGKPYTGVNVLLLWLAADEAGYVSNDWLTYRQAQQLGGHVRK
                     GEVATLAVIYKDWKKQAVDEHGNRLTDSEGEPLMEIVPVLKSNPLFNVGQCDGLPEHL
                     MVKAEGSADALPADTFSERVLQVLNGSGVSVSSLPQDRAFYRPDLDRIVMPLSSQFDQ
                     VADYWTTLLHELVHSTGHEKRLRRVGISTFTGRNSPEYAFEELIAEMGSAFLCAHLGI
                     AGDVQHESYIDGWLKVLKSDKKALFRACRQAREASEYLLTRRTSQECAT"
     gene            27323..28270
                     /locus_tag="ACHK74_002499"
     CDS             27323..28270
                     /locus_tag="ACHK74_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFP0221303.1"
                     /translation="MSEWCKNRFEITGKSVCLDVLTQWIEGRETPRYRHAIQQSIQLF
                     LAGCAGLVKPTRTTQYPPYPALVRHGTGLSSPANQAFEQWLGLLVKNAVLDGETIKTI
                     ERLYHQSCIGAVRWGNLPDNAREIITTLMSRQYSDWFGLAGLSEHIDAEACWSWLSDY
                     PEQAQPCDMLMVIPSRLATELNGSGGLLQGISTTTSLYSRIYGVEWPSGNNVRHARDE
                     MSSLVLLIDTPWYPPSGELVGEISKVFDCEVRHWYSEPVHGIQGYNCYDGGEHTDSDL
                     QAEWPGRETLPQPRLYLVEECAEEQTKAAPLPVPLVSAQ"
     gene            28382..29068
                     /locus_tag="ACHK74_002500"
     CDS             28382..29068
                     /locus_tag="ACHK74_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP0221304.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPRSVASMMAQMQLGNVKVLFESKPFITLSEPACGAGSMILAMADILNRSGYPAY
                     HRMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRVLLTPGHYLGNWDHR
                     LRHLQEQNVA"
     gene            complement(29308..29860)
                     /locus_tag="ACHK74_002501"
                     /pseudo
     CDS             complement(29308..29860)
                     /locus_tag="ACHK74_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154742.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            29978..30895
                     /locus_tag="ACHK74_002502"
     CDS             29978..30895
                     /locus_tag="ACHK74_002502"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFP0221305.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPG
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGIINTPVCRSPFSP
                     VKEKNLDVLSKLAERLLEEHDRNKKMKII"
     gene            30928..31802
                     /locus_tag="ACHK74_002503"
                     /pseudo
     CDS             30928..31802
                     /locus_tag="ACHK74_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009384 - N-acylmannosamine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
     gene            31851..33323
                     /locus_tag="ACHK74_002504"
     CDS             31851..33323
                     /locus_tag="ACHK74_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0221306.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAGE"
     gene            33327..34157
                     /locus_tag="ACHK74_002505"
     CDS             33327..34157
                     /locus_tag="ACHK74_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0221307.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYEHYITDNSLFENTDDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEETSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLLEWLAEHKFH
                     "
     gene            34203..34913
                     /locus_tag="ACHK74_002506"
     CDS             34203..34913
                     /locus_tag="ACHK74_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFP0221308.1"
                     /translation="MKKYYPLLTVILLGLPLCSQALTLNLRGGYRSASHAYETLIQVS
                     QGWNNGWWASFESNTWNTIHNNENELFALNYNKIDINKTYKIDDKWALIPGIQTQWMS
                     AGSQYNPYLKISYQATPDLNLALRYRYDWKAFRQTDLDGNRARNDQHQFDGYVTYKIN
                     DSWLFAWQTTVYTKVNNFKYGNHKKTATENAFVLQYKMSPVFTPYIEYDYLDKQGVYK
                     GKDNKHENSYRVGVLINF"
     gene            34926..36035
                     /locus_tag="ACHK74_002507"
     CDS             34926..36035
                     /locus_tag="ACHK74_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFP0221309.1"
                     /translation="MKVKNFIYLPFCLFIGTSVAGALPEIPEPFKYGVGGIDNGKIYI
                     GLGSLGNNWYMIDTNQSEKKWTKIAQWPTVPREQATATIIDGKIYVFGGIGKDKSGVI
                     TLQKDVYSYDIAKDKWEKLMTRPPVSLAGHVSFIHNGHAVSTGGVNENIFNGYFSDVE
                     LSKGNSALTEKVNRDYFSKPVDDYFLNNHMISYDPSKNQWKNLGTTPFPGTAGSSVIF
                     AEQQIYILGGERKPGLRSVRSWTGELSHDRIKWSELPPVASPEGVSGAYASVIDGNIF
                     LAGGAYFPGAAEKYSNGEYWSHKGLDKAYSKEIYQLIKNDWKKVGSLPEGLAYGVSLP
                     WQEGMLILGGEKKGGKAVSDVIYLKKNDKQIKIVK"
     gene            36085..>36412
                     /locus_tag="ACHK74_002508"
     CDS             36085..>36412
                     /locus_tag="ACHK74_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0221310.1"
                     /translation="MEFIMNNDKSLNEMMLSLFNKSTIVDLSSETISESKSLSGRIYQ
                     DPEHNGEFELDRVLTDIYAPQLIIFLPDKPNGTGILIIPGGGYRAVMLDKEGTALAPE
                     FLSQGYT"
BASE COUNT         8819 a         8446 c         9451 g         9696 t
ORIGIN      
        1 ggctgcatct gccggtacaa caatttatac cgattcgcac cagcgcccca tgaacccgcc
       61 agcaggtgtt cgtgttgtcc tgcttgatgc tcctgagcag acgcaggata catttttcgg
      121 ggtacttccc gcagagcccg cagaagcatc tgcccgtgtc atggaacgga tgcagtcgcc
      181 tgaatggcag tcgtttcagg cagggcttgc tgaacactac cgtgcattag cacatgcctg
      241 gtcccttgga ctgaaaaaat atccggctgt ggtttttgat gacagtgaag tggtctacgg
      301 aaccacagac gtggtgctgg cagaacaact gcgtacaggg ggagggcagc catgatttcc
      361 tgcacatcac gtcatcgcgc cggtgttctg ctgttaagta tggcggtatt gccggtggca
      421 gaggcatcgc tgaacaccgc gcagatagtg gccagtgcag cctcacagaa ctgtatcagc
      481 tggcgggtca gcgggatttg ctactggctg ttctgcacca aattcagttg caaggtcaga
      541 acatcagtga aagtttccca ttatatcccg gaagtggttg tttccaccta taccgcacct
      601 ggcggaaatc cctggcgtga aatgtcggcg gtcagcagta cagccggtgg tccggagaat
      661 gccataagca aaggtattgc cggtctgtca gcaggaggag ggaaccccgc agacatgaag
      721 gtacaggggg aacgtcgttc gtcaatccgt tttaaatatg ccgatgcaac agggcacccg
      781 gccaccacgc ttatcggagg gatggtgccg ggatattcct gtgagtcacc ggtgacacct
      841 tttgttcctt attttctcag tacgcttgac agtattgcct ggcgtacagg actaccggag
      901 tcactgtacc cggaggccct gatccccggt aaacgcgaaa taggcagcca ggaagaagag
      961 aatatgtggg gcaatgtgta tccccgctcc gggttcgtga cccagacaga tgatgataaa
     1021 gctgctgcgg tggtggccca gcgggtggct gacattatca cccgcaccgg tgagccacat
     1081 gtctatcagc cggtgaaagc agggaagaag gacggttact ggccaccgga tccggtggag
     1141 gaaaatacgg gcacccggaa ccataaatgg cagagactga cgccttcagt ctccacgtcc
     1201 tgcgcagtct tccctgacgg agagcatgaa gcggctgaaa caggtaatgc tgcctttgcc
     1261 ctgtggcagc cctacagttg ctgcaagaaa cggggacaga cattcctggg cagtacgaat
     1321 ttttaagtaa ggggaagtga tgaaacagaa aatgacgata cttgttctgg ctgtggtgct
     1381 ttgtgcgggt acggtacagg ctgacggaaa ctttttgtcc ctgccgaaag tgaatgacag
     1441 tgcgtttggt tacggtaaat ccgtggacgg cgcagtatca gacaaactgt tttataccct
     1501 gggagggggc tcggtgatat cgcagccggc aacgcgcagc aatatgcaga aacttggggt
     1561 ggatctcggg tggagcagtg atctgatgtg tggcaatttt gacctgaaaa caacagtggg
     1621 taaccagctg aacgggatca ctgatggttt caaaaacctg atgggggatg tgattcaggg
     1681 ggcgagcgga gccgtggcga gtctgccggc aatggtgata cagcgggcta atccggggct
     1741 gtatgacatg ctgaccaacg gtgtactgca ggcgaatgtg gcattcgaca aagcgcagct
     1801 caattgtcag aacatggcga aacgcatgat ggagttcacg gacagcaata agtggacgca
     1861 ggcggccatg ctcgaggagt acaaaaaaat tgtgaatacg ggggacggtg atgcgatcaa
     1921 aggggatgaa gcaggtcgtc aggttaccgg ggaggagggg caacgctgga tcggagggca
     1981 gcagcgtggt ggagcgggac agtctgctat ccggccgaca catgatctga cagctgccgg
     2041 tttcaatatg atgaacagtc tgccttcgac cagtacatcc agtgtcagtc ccgggaactg
     2101 taacgggagt gcctgcatga aataccgtaa tgcagaggaa gctgcccggg cggtggtgaa
     2161 ggttctgggg gataaatcca tgcggacctg tggtgatgca aaggcatgta ccagcgggga
     2221 tgctgatcag cagccgggga ccagtattgc cggcacgggg tttgcgccga tgcttgaaga
     2281 agcgacgcgg gttaacacgg aactgctggc aggtatggtc aatggtcgtg acaagccgac
     2341 agccgggaat ctggcaaaac tgaaagccgg gagtctgtct gtgacagcgg gagtgatccg
     2401 tgccctgcag cgtgatccgg acaatggtgc cctgacgtcg cgtctggcgg gagaactggc
     2461 tatggcggat accgttgaaa cggcgctgct gatgcgcagg atgctgctca cggggatggc
     2521 cgagccgtat gctgcagctc agcctgctgc actggaagag ggggaacgcc gtattgaggc
     2581 gctggatcgg gaaattatgg cgctgaaaaa cgagatggag ctgaagcggg atctggcgcg
     2641 taacagtgtt ctgaccatca ttgaacggga taacgagcgt gtggggaata acccgatgat
     2701 ccagcaggct gacaatgctg acagccgtat ccattctctc gatgccccgg ataaggagtg
     2761 agaggatgag cagaatttat ggccgggtga aaaagaggat gaaggtattc gccctgtata
     2821 cgctgttgct gacagggatt accgtgtttt ccctctggct ggccgatacc ggtatacgtc
     2881 acgcagaagg atttgcggcc ctgcgggagt ggatgtatga gacacgttat ggctggctgg
     2941 gatggcgtct gtgtctttac agtgcgatcg gatggctggg gtggaggatc tggcatgcgc
     3001 cggggttcag ggcagaatac cgggcttctt ttctgcgtat ggccacagtc agtgttctgt
     3061 ttatcctgct gtgtgaatac gccatttttg ctgagccggg gcgttgagca atcatgacca
     3121 caaataacta tctggaatat tttctgactc tgctgggctg ggtggtgaat aacggtctgt
     3181 ggaatgtgtt gatcagcaca ggactgtttg ccctgccact ggcattcagg atcatcggga
     3241 tctggctgaa agtcagggaa gaaggtgcgg atgagggaaa caaagggatg ctttccctgc
     3301 cgcgtattga gcatgttctc tatggtgctt tttttgtgat gatatcctgc tgtgttcctc
     3361 tggtacaggt gacgctggat acgctgtcat tcgataaatc cagggcacag acctgtgggg
     3421 tatggacacc tgaagagccc ggagagacgg ggtacagtgg ggtgatcagc agtatagggg
     3481 atcagactgc tgcagccccg gtatggtggg tggtggttca taaattgtcg aaaggtgtca
     3541 cccaggcggc agtggcttcg attccgtgcc ggcctgattt gcgccagttg cgttttgaga
     3601 tccagcgaac gtttatagaa aacagggcac ttgctgatga gctgcaggac tttacgaacg
     3661 actgttattc actggcactg tatatgtgga aacagcgtga tcaggggcag agtaaagata
     3721 aaaaagtgct tgatgatatc tcctggatag ggagcgccac attcatgcag ggggattacg
     3781 ggacgctgca gtcaaagatg ccgcgggctg cgttcccgtg gaatacagca cgtgacagcg
     3841 gacgaccgga tacggggcgg agaggctatc cgacatgccg ggagtggtgg agtgacagta
     3901 gtgtggggct gaaaaaacgg gtcatggagg aggtcagtga ccattactgg ttacgtctgt
     3961 cagctgcaat gaagatgatt ggcagtagtc aggtggatta taaggaggag gtgatcaagc
     4021 gtctggtgag tccggcaaac ctgacgctct ctcagggggg agaggtgtat cgtggctatg
     4081 gtggcaatgc agatatgacg actatgaatg cggtggcccg gattgcggga acggcgggca
     4141 gtgcggtagc agcactgggt gcgtttccgg cactggatgc aatgcggcag gcactgccga
     4201 tggtacagtc gataatactg atggcgattt acatcatgat accgctgatt ctggcgtttg
     4261 cggcatatga actgtcgacg ttaatcacac tgacgtttgt ggtgtttgcg gtacactttc
     4321 tgacgttctg gtgggagctt gcacggtggc ttgacagctg gatgctgacg gcgctgtaca
     4381 gttcggatac acatacccgg ttcaatatga tgggattcca gaacacatcg gatgatctga
     4441 taatgaatct ggtgatgggg acgatgtttc tcgtcttgcc ggcagtgtgg ctgggagcgt
     4501 tgtcctgggc tggtgttcat attggcgatg gaatcagtcg aggattaccg aatggtattt
     4561 cggaagcgaa aggtgcagct tcgagtgcag gaagtattgc taatcgaggt ataaaatagg
     4621 aagtaaaaaa gttacaggag aatttacaga aaattctcct gcttaattac aaacggtatt
     4681 ctgttcctcc actaatgatg tagtcaccat cgattctgga ttcaaacttt gagcatggat
     4741 gctcaatttt aatgaattca ttattacgta atttgttttt attttggtgt gaaactttgt
     4801 ctgatgcagg taaaacagca gaaatagtct tttttagtaa tgaatccaac gtccatggat
     4861 tatccacagg cttacaacgc cacattgctt tttcagattc gttattcata tctataccct
     4921 cagcagcagg ataaataaca cagtgttatc atacgtcttg tatgataaat gtgcaatcac
     4981 atgtcttcca tttgatgaga ggctgcgata ccacattacc ctggggctcg gggattctgt
     5041 tgaaatgatc cagatgagaa tgtttcttat cgttagatta tgagtaagcg tcccattaag
     5101 ggggatagtt gccagactgg gcaactatca caagacgtct catattgcgc gctttatctt
     5161 ggtcagaatt tcatcactag acatgagatt tcagtgtcag tccttcacga gcaacgcaat
     5221 cacacgagtt ggtacttcag tgccagcctc cggtacttgt ctggtgataa cccttgttta
     5281 acaaaataaa acacgaccat catcagactg cgatcattcg tatgaaccgt cgatgacgta
     5341 tatgaaaaac tcagtaatat aaattcgcca gcatattaca tttgtttttt tgatggtgat
     5401 caaatacatt tagcaatgta tgttatacat agaatataag tgtggaataa ttttcataaa
     5461 ataaagatga tgtttgatgg atgttgctat gaaaaacgcc aatcgagtag taattaatct
     5521 ttttagtata tctcttgtga ccatgatatc tgggtgtgct tcttattggg ggaaagttcc
     5581 ctataatcca ccgtcagata cagagaagaa catagcgcat gtcagattaa tcggtaatcc
     5641 accaggtttc acaataagcc agtatggaaa taagcctgct gcggttgatc cttctaatat
     5701 ggtgatttta agaccaacac acgatttagg ttttccaaaa gtaaataacg atcccgaacg
     5761 ttataaagag acatattatg atacaaatgt ctatgctggt actccgacac ggattggttt
     5821 caagtttctt catggctgtg aagatgtagg ttatactttt ttaccggaaa agggcgagac
     5881 ctacgaattt cgattgtctg tcactgataa aacagggtat tgcgttctct atgcattcca
     5941 cctgaaatat gattcagaaa ataaattgta tataactgaa aatattgaag agtataaagg
     6001 ctatgtttat cgtgaataag atgtgtgtct atttgagtgg aggatttctt ttgtgtctgt
     6061 tagtcggttg tatcgggcat attgatagaa ctaatgaaac aacgctgggg aaatactcaa
     6121 agcaagaaat attcaataaa ttaagaaagc agaaaaccac taaaagagaa gttttatctc
     6181 ttttagggcg tcctaattac ccatatgatt ataacgacaa aaatacatgg atttatttat
     6241 caaaaacaga ggggaaagtg ttgtatgggg ctattcccgt tgatttcaat cgttcagtga
     6301 tgctaaagtt ggtatttgga aaagacaaaa ctattgaggg tatagaatat aaacaataat
     6361 aaaaaatctg ctaatgtata acagacaggg cacagcagca gataacggga tactatataa
     6421 tacctgtcag tccatagaag gaagttatac gaataccgga gtgctaatga acagaatatt
     6481 tctgtgaaga tgacttcccc gtaacgacac cacaaagcca ttgtctatgc gctgtttcat
     6541 cgcgtggaac tgagccgctt cctggaagat ggtgcggtgc cgctggataa taatgtgtgt
     6601 gaacgggcca tcaaaaacgt ggtcctgggc agaaaatcgt gtctgttcgc cggttcgcag
     6661 atggcgggag aacgcgccgc gcaaataatg agcttgctgg aaaccgcgaa acgcaacggt
     6721 ctggagccgc atgcctggct gacagacgtc ctgatgcgtc tgccggagtg gccggaggag
     6781 cgactggcag agttgctgcc tcttgaggga tttaccttct ccgggtgagt gatacctgcc
     6841 gtcaggtgtt cgtgcaccgg gccataacct gcagtcggga gttgaacgcc tgacgacagg
     6901 aaatgagcca gaccagcaac ctcacgggct gacagcgaat aagaacctga atcagcccgg
     6961 agtcattccc gggtcagtac atcatcacct gccattaatc cccgagtcag tacttcgcgg
     7021 cctgacgcga tcaccctgtg caacaaaata aatcaaccgt tatcagacca cgatcgttcg
     7081 tatgcgccgc cgatgacgcg cgcctgaaaa acgcgtcaag atgagatgcc cggaagcact
     7141 ctgctgactg ggatcaaacg ccagaagagc agaggtaagc aaaaaagtcc gccaactaag
     7201 gacgggatta aatttatgct ttagggactg atggcggaga ttttctggct ttacgttcat
     7261 ctatccatgc aaagatccat gatcttaacc cagctccccc ccctgcgatc ccactcaatg
     7321 ttatggctgc tttaaattgt ttttctgaaa aatagaaaca acctccttcc caaaaattca
     7381 caatgagcag cactacacta ccaattaaaa aaaatatcac ccagcaatag attattgtta
     7441 gcagtaataa atatactgaa ggttttttac tcatgttctg gctccttatt agtaggagta
     7501 ggataatttt taataccgta gtttattaac tcagaaacaa agccaccacc aatatcacca
     7561 acagtacttg cagagttacc taacactggc ctcaatatca cagggaaata ttctcccaca
     7621 tatccacctg cgtatgcccc gagagcagtt ccagattggg acttaatatt agcaccatcg
     7681 ctaaatacag atccccccag cgctatagct gtattagctg taactgtacg acctggagca
     7741 agcatccctg tcatgaatga agatgcgcta cctttataat cccatgactg attttcacta
     7801 ccaggttggc tcaatccata ccattgaaaa ccaccgttca cagcagcgga agtaactcca
     7861 ccgaccgcag ctttacccac tgacaccgca ggccccagat accaggcacc tgcaatcaat
     7921 acgccatctt tataaccatc aactataact ttgcttatat tggctccttc aggtaactca
     7981 cctttggcaa gtttatccag tcctgcctgc ttctgttccg gggtgagatt attggcatca
     8041 gcatactgat tccaggaggc aaccgcctga ccataactca ccattcctga tggcagactg
     8101 agcgcattat tctcaacagt cgtcttacct gactgtgcgc cggctaccgc tgaagctccc
     8161 gaatctccca ccagaccacc ggccaggccg gcagcaaccg ttgccagtgt cgagaccgtc
     8221 tgcttctctg tctcactcag ttcactcacc ggctgaccgt acatctgtgt ggctatcatt
     8281 cccaccacct cgccggtggc tgcgcctgcg gcaccggcaa gagcattctt accctgtgcc
     8341 agagacaggg ctgcattcac tactgcatga gctgccacac gtcccgcacc atccggggtg
     8401 gtctgtttga taatttcagc aatatacggc gcagcaccac ctgccagggc tgcactcaga
     8461 tttccgccgg ccagtccctg tactgctgcc gttgcggcct gtatcgcctg ctggtatttg
     8521 cctcccgttc caaaaccgga ggctgccata ccgttgttat atgcccgctg aaccacatcc
     8581 tgttccgtga acggttttcc tgctgcctca agctctgcgc gggcctgatt cagtgctgcc
     8641 gggtcccgct tcgctttctc accagctatc tggccttccg ttctgacaat atctgccacc
     8701 tggttaccaa tctcaccaat gagctgcgcc atctgcaacc gctgctgctc tttttccctg
     8761 tcaaagatgg gggacagcgt ctgatttgca tgtgctgcgt cacggttcag gtctgccaca
     8821 tcctgtttct ggctcttcgt atcccggatg gtgatattcc cggcagacac tgccgactgc
     8881 gtggtactgt ccgcatggcc ttcatgattt gcaccgacca gcagattgtt tgccatgttc
     8941 cccaggaaat ttccggcaac actgccaccg gtactgagcc ctgcactctg atgttccgtt
     9001 ttaaagtcgg cccggttttc aaggtcactg aagcccagtg tgccggtatc cagcctgttt
     9061 ttatctgccg ttgccgtgga cgcaatcacc gcaccgttca gttgcgtatg ctgtccggtg
     9121 gtgacatcaa acccgcccct gccggcaaaa atccctgtct gctcctgcac actgtcatag
     9181 ttactgtgca ttttatcccg gctgaggctc acactcgccg aaccgctcat ggacccgaaa
     9241 gtgaagctgc ctccggcact ggcattctgc tgcttactgt cataacggtc actgtcctgc
     9301 tcactggtca gtgtcagatg gcgaccggca tccactttca ccgtttcacc gccggcctgc
     9361 gcgccggtaa gcgtggtgtc ccgcccgctg ataatggtca gccggtttcc cgcatccacc
     9421 gtggtttctg tatgggtggt accgttgcct ttttcactgc ccttaccctg gtttgcgctg
     9481 gctgaaacac tgatacccca gcctccggcc cccacaccga taccgacacc cacactgccg
     9541 ccaccggatg tgttctttcc ttccagcttt gacgtgtttt ctgctgatac cagattcacg
     9601 tcacggtttg cctgcagcag aacgttgttc ccggccttta tctgactgcc ctgtatcgtc
     9661 aggtcaccat ccacgccctt cacgccggag ccggtggcct gaatactgag gtgattacct
     9721 gcggtcagtg tactgccctt cgccaccgtc tgctctgact gctgttctga tttcgaagac
     9781 tggctgccgt aagagaggct gatgcccacc gtgttattat ttccggcatc ggctccctgc
     9841 gcgtctgcca gtcgtccggc ctgcacggcc tgagcccccg agagcgcggc cttcaccccc
     9901 tgcagggctt caagccggct gtttccggca gatttcgccg cctgtacggt ctccaccgtc
     9961 gtgttcaggg cgctgcccgc tgtgccggac agggcaagcg tcagaccgga ggttttctgc
    10021 tccaccgtat gtgtctgtac atgctggtct gtggcggcgc taatgctgac ctccttacca
    10081 ctgagtgcca tgtccctgcc ggcaatcagg tcagcactgt tggcgctcag ccggttacct
    10141 gcctgaaggg aaacatcacc gtgaacactc cccaccgtgc tgcctttctg tgtggtgtct
    10201 gttgcggtgt ccgtgacttt cagactctgg ctgcccacgc tgaatcccac acctccggag
    10261 ctcatcagcc cggatttttt ctcctgtttc aggtgtgttt cacggttgct ttctgtcagc
    10321 gtgccgatac tcaggtcatt tccggcagcc agggagacac ggttatcccc ggccacactg
    10381 ctgcccgtga cgttcagatt atgtccggca ctgatactga ccgtatcccc gttcagttca
    10441 ctgcccgtca tggtccggcg gtcagtgata tcatgcgtgg ttgtggtggt tttcgataac
    10501 atcccgctgc tgccggtcac cttatggtat tcatccagcg agctggtatt ctgtgcaccg
    10561 gtcagcgtca cgtcatgacc cgctatcagg gccagacctt tctgagctga cagcgatgcc
    10621 gcctgtgacg tcatatcccg tccggcggtc agcgtgacat caccgttacc ggcaagcgtg
    10681 ctgcccgtac tctgtgtgac gccctgcgac agacgattat ttttgtccca ggtaatattg
    10741 tcctgcgcgg atgtggtcac tgtggaaagc gtcatatccc tgcccgcact gagttgcgtc
    10801 aggctgtcct taccctgatt caccaccgtt gcagccgtca gattcatatc ccgtccggcc
    10861 tgcagcacca gtcttccctg gtcgttctgc acatacacgc cggccacact gtcgatactg
    10921 gtacgttcaa aatggttctg tccggcgtca ctctgtgccg tacgagtggt ggtggtcaga
    10981 ctgatatccc gtccggccat cgccagcagg ctgtccgtgg cctgcagcag cccgccggta
    11041 ctgttaatat ccgtccgggc cgtcaggctg acatttgctc cctgaatgga gccggcctgg
    11101 ttgtgaatgt tttctgcact gagctgcacg gtctgcttcc cggccagttt accgctgtta
    11161 aacaggtcac catccagctt catcaccatg tccctgccgg caatcagcgc accggcactg
    11221 ttcacatcac cgggttttac acgggcatac acctgaggta cctgaactgt ctgcgtgctg
    11281 ccgtccggca gtgtcaccgt ggtgttaacc agccagacaa tatccccggt cagtaacgcc
    11341 atctgctcag ccgtcagcgc cacacccggt ttcaggttgt attgcttacc aaatgcaata
    11401 cccgcatcca ttaatgcctt aaactgctct tcatcactgc cgtatccgtc caggtaccgt
    11461 tgcccggtca gagctacaac ctgctctcga atcagacgct gctcgtaaaa accgtctccc
    11521 agacgtttga gcatgttatc cccgttctga gaaaacgcct tctgcatgta atcactgccc
    11581 agccacatct tctggttcgt aaaacgcggg tccgtttcaa ccagatatgg cacattcact
    11641 gccggatttg ttttaaacag actgctgtcc ggcagacggg tatccggccc ggcaacatgg
    11701 atactgccct gaggtgtgct gacttcaaac tgctgccccg gcaacagaat gaccggttca
    11761 ccggcagaca ccaccgggcg aagcggtatc cggtcagtag cggctactgc cgcatccaca
    11821 tttccggcct gtcctgtctg tacatccgtt ccctgtggct tcagaggaga aagcgtgacc
    11881 tggctgccct gaatctgtcc gtggctggtg agctctcccg gcttcagcgt aatgctctga
    11941 attacagtgg gcggagtgta ctgagaggtt ttctccccct gtttatcctt tccttttttc
    12001 ctgatacggt aataatacgt ggatgttcct gagtccgtaa catgccgctc gccactgaca
    12061 ctgacatttt ccacgcttcc tgacggaatg gtcagcaggc cgcctgcaac aacctgactc
    12121 ttatcattaa gcagtttgtc tgccacaatc gtcatgccgg caccggacag aatttttccc
    12181 gggtcggagt cttttacccg ggtttcttcc acggtacggg tgtaatcgta ctgcgtgaat
    12241 ttatctctgg caccttccgg tgtatgcagt tttttcagtg agttactgga atcgttatcc
    12301 acataaacgc ccggttcacc ggcgctccag cgtttatctg agcctctgag ctgatactca
    12361 gtcacctttt ccgtggaaac caccacgcgc tccgtggtga aatggtcatt gacgttattg
    12421 atttgtccgg cactcagtgc cagatatccg gcggcttcaa ttgtggcact gtgattattc
    12481 agtacaccgg ctttccccgt ggcggcgcca ttcgcatcca gcgtgccacc gatatgcata
    12541 tcgcctgcac tgtaaatcag gctgtgcgtc cggttattca gggtctgtac ccctaagtcc
    12601 acacgctcac gtccggccag cgtcgccgcc acgccatttt catccagatt attaaatgtg
    12661 gcagcgctgg caccaacagc atcgccgtag attcgcccgg taccggaatt cgtcagggtg
    12721 ttcgcctgaa gatgcgtata tttcccgtca atcagcccac ggttcagcag tgtgtccgtc
    12781 acgttaagcc aggtctgccc ggcactgatt tcaccttttt ctgtgttttc aaggcagatg
    12841 ctgttcagct caagccggct gcccgccagc aggctgccgg tattagtgac atcgccatta
    12901 acggaaagcg taaggtttcc gttcgcaata gtctgaccac tgttactgaa cgctgtgtta
    12961 cttttcagcg tcatatcgcc cggcgaaagc agcttcccgt caccaccgag ctgaccggca
    13021 tccacagata ctgactgccc ggccttaacc acgccgtcac ggttcgaaaa cgccagctga
    13081 cggccactga cagacaatgt cgcgccggaa gataggacgc cgtcattatt actgagcgtc
    13141 ctgtctgcca taaccgtaag cgccctgtct gccagaatac tgccctcctg gttatcaagg
    13201 tcctgcgttg tgatgtgtat tgtctgcgct tccagcccct ggccggtatt ctgcgtgtgg
    13261 cgattgataa actgcagtgc attcagcgta atggcggaac cactgcgaat cagaccagag
    13321 acgttgtcca caacgccccg tacactgtcg agcagcatgt cgcccacaga ctgtatctgt
    13381 ccgtggctgt tctgcagacc tctcgtatgc agacggattt caccttcact gaccagccgg
    13441 ccaccctcac ggttatccag cgaggtggtg ctcaggtcag ctgtcgtttt cgcccccacg
    13501 gtcccgcccc ggttatccat tgctccggaa ctgacagtca gttcctgcag gctgttcagc
    13561 gtaccgcgtc tgttatccag cgtctggcct gcggtattaa tgctgatacc ccacccctga
    13621 ataagaccgg actgattgtt cagcgcctgg ctgttccagc cgagcagacc gtttgccacc
    13681 acctgaccgg atgtattgtt aaccacgcct gcagtcaggg acagagcatc tcccgacaaa
    13741 agcactccgg ctgtgctgtc cagtgtgccg gaattcagtg tcaccgggcc ctgactgata
    13801 accccgcccc ggtcaccact gtcacggttg ctcagtgtgt cagcacggat attcagcgat
    13861 gcaccgctct gtatcaggcc gccggcatcg ttgatgatgt ctttcgaggt aagtgtcagt
    13921 gactgctgtg ccacaagcct gccaccctga ttatcagagc gggtgacatc cagtctgctg
    13981 ttaccgttgc tgaccaccgt cccctgctgg ttttccagca cattcaggtg cgcattcagg
    14041 tctccgccgg cggcaagcgt cccttcctga ttactgacat cgtcaccact cagcaacata
    14101 tccgtcccgg ccttcagtgc gcctttctgg ttcatcagcg tacctgacga tacctgtagt
    14161 gacgcctggc tccccagcgc gccctgacgg ttatcgagcg ttccggtgtt cagggtcaca
    14221 tccttctgac ccgccataaa tccctgctgg ttattcagca taccggcccg tactgtcagg
    14281 gtaccggcgc tgctgatacc accccgtgtt ttcgtgttcc ggttactgag taccccgtcc
    14341 tgtacatcca gcgtcatgtc actgccggac tgaatcagac catcatcaga gaccagttcg
    14401 ctgcctgaca cagacagcgt tttctgtgaa agcagtctgc cggacgtatt atccagcgca
    14461 ccatccagcg tcacagacag ctcgtcagac accactgtac cctggtggtt attcagattc
    14521 cctgccgaaa tatccagcct gccggcggac tccattcgtc ccgccgtatt gctgacagcc
    14581 tgctgtgttt ttatcctgcc cgttccggca gcagctgaaa tcagtccgtt gtcgttattc
    14641 agcgcctgtg tattaatcag gatgtcacgg ctggcaatca gagttccctg caggttatcc
    14701 acggcgccgg ttgtcttcac ctgcagactg cctgactgcg ccgcctgcac tttccccctg
    14761 cgattatcaa ggctcagtgc atccaggcgc accgcgccat ttgccgcaag cagcccttcc
    14821 cggttatcca cgcttcccgg cagactcagt gaaaaatccc ccgttcccgt ctgaatcagg
    14881 ctgcctttat gatttgacag gctctgtgcc gatatctgca gtgtgccggc atttatcgtg
    14941 gcgccattat tgctgaactg cccggcagtc cgggcagcaa aggtaccggc gctcagttgc
    15001 gcattctgtg tggacagatt tccgctgcct gcatccagcc cggtatgttg tccctgtatc
    15061 cggctgttgc ttaaatccag cccctgtccc ttcagttgca tgtccccacc ggacagaacc
    15121 tgtccgtgtg ccgccagttc gccggtggta ctgacagtca ggttacccgc ttctgccaga
    15181 cgtccgtcat ctttcacccc ggcggccagc agactccgct caccgccggt cagacgcgtt
    15241 gtctgaatcc gggtgttgcg acgtgatgcc acagagcctg tatgggtgaa tacaccatca
    15301 ctctgtatct gcgtatcctg ccccgcataa acagaaccac tgttattcag ttcccgggtt
    15361 gtcgccagat gcacatccgt tttcgcactg atactgccac tgttttcaat acgtccgtca
    15421 gccgtcaggg taaccgcccc ggcctgcgca ccaatatgac cggcgttacg cacgcccaca
    15481 cctgtttccg taccgacaag gcgaattttt ccggcataca ttccaccaag gctggacaca
    15541 tccagcgcca cctgtggctg cgaagaggca tcagcggctt ttttctcagt ctgcccgtgt
    15601 gcaatatcca cattgttacg tccggtggtc actttcaggt cctgcgccca gacacccgca
    15661 ttaatcttca cagaccgggc aatgatatcg gtataatccg tgcgcgaggc atccatccct
    15721 ttgcccgtaa cctgaatctc accaccttca acacggaatc cggtcagact gccgtttttc
    15781 atctgcggct gccccgtggt cagcgtggcc cggttcgcat taataaagcc gcacccgtcg
    15841 caactgatac cggccgggtt tgcaatcacc acctgcgctt ttttcccggc aacctcaaca
    15901 aaaccgttca actggctggg attgcgactg ttcacttcat taagaatgat tctggcctct
    15961 cccttagcca gccacgggtt cccggccacc atgccaccca gctgagtctg cacattgtta
    16021 tgggcattat tcagaatgac accctggtta ccgacgtcga actggctgta tgtgttatgt
    16081 gacaccccgc cggcagaagg cgtctgaata ttcacctgcg gaatgccgct ggccgtctgc
    16141 ataatcgtgg gctgctgatg ccccggtgcg ccatggtctg cgacaatgcc tgcagcgctg
    16201 acagaatgca ccatccccga ggccagccac agggagaatg caagcggcgt gacacggcag
    16261 atacgttgcc tgcgtggata tcctgttcgg gaggagggtg atgcaccggc gcgaccggaa
    16321 cgggcaatat ctgccaccac catcagcatt ccccgggctt tgttaaaaat aatgcggtaa
    16381 caatttctgt tcattctgat aaacctgact tatctctccg gcacatccac agcaagcggg
    16441 tgctgcacac gccactgtcg cgcctcttca cgactgacct gcgcaccccg gaaattcatc
    16501 acccgcaacc cggtctgctg cccacggtgg tacagcgaac gccagcacag acggggaaaa
    16561 atatcctgcc ggacaagacg actcatatcc ggagtgtgtc caaaaggggc aacccagtca
    16621 cagacccaca tccggtcgcc gctgttccag tccgactgag gcatgagaat tgccggctgc
    16681 gtaagataac gcgcctctgc ctccgggctg agccaggcct ggctcatgaa gaaaacaggt
    16741 cgcccctgct ctgttgccac aacatactgc cggttcttaa tcaccggcag cagcaggtcc
    16801 ggcagcgtat gcagtggtgc atcacgatgc aggggagagt gcatccacaa ccacacgacc
    16861 gctcccagca cttccgcttc attgaccggc tcaccggaat acagggggct cagaacgtca
    16921 tatttcccgt tacgcatgaa cgtttctcag aaattcagga acagattaaa accggctgtc
    16981 acatcactcg tcataaaacc cgccggtttt gaaaacggac gaccgatgaa aacgtcataa
    17041 tccgtactga gcagtcgccc cctcagcccc acaacaccac cggccagatg acgtcccacc
    17101 aggtattcac tgccggctcc gcccacctcg ccataatcca ttcccagata taacgcctgc
    17161 tccggcagtg gcgtacgcca ggccaggtca ttgcggatat accagccgtt actggcattc
    17221 agcgttctct ccccgtcaaa accacgaacc gtccagcggt taccgatggc aaactgttcc
    17281 tgtggagtca gcggggtatt gctcagctgg cgctgatacc ggaaggaata actgaagggc
    17341 tgctctgcca gtgtaaaggg cagattaaag gatgcattta tatgcaggat cttactgagc
    17401 gccgttgcct cgccgaacgt ttcttccggt gccggctgtg cgccagacca gcgggttccc
    17461 cgttgccagc tgacgccggc atccagtgtg gcctgcgaga tataatggcg gtgctgaagt
    17521 ccaagtcgcc aggccgccgt ctgtcggcgc tgtacctgga tttccgtatc attaatgtag
    17581 ttacgcgacc tgcgggcctg aacatcgtaa gtcagcaccg ttttctgcgt cccgttacga
    17641 tgcagtaccc ggctcagctg catgcccaga ttctgactct cgccactgta ctggtaatcc
    17701 tcaaccgcac cagctaccgt ctgatggtaa tcgtaatcac tggcggtgac ggaaaactgc
    17761 cagtagccga aaggcacaga gtaatgggcc gtgtagtttt tgctgccctt acccccgtaa
    17821 tgtggaaaat tatgggtggc agaaacatac agcaggtcgc tcagggaaaa aggattatcc
    17881 agagaaaatg ttataccacc gagataacgc cctgtgctgc gggtacctga atcatcaagc
    17941 gaaaacccca gtcgccagta tttgtcctgt ttgcgggtaa taagaatgtc actttcccct
    18001 ggctgctcac cgggcacaat ttccatatca gcctgcacac ccggcagtcg ttgcatattc
    18061 tccagcccct gctcaatgtc ccgtaaatcc agcagagcgc cttcgtgtgc cgggaatgag
    18121 gaatacaact gaatatagtc atcactgtcc ggtgtcagac gcacatgtcg cacgacaccg
    18181 ggaataataa gcagccggag aataccgctt tttaaatcct gcgaaggcgc cagaacccgt
    18241 gtggtgatat aaccatgatc gaccagacgg ttctgcagtg tactcatcag caggttgatc
    18301 ccttttgccc ccagacaatg tccgaccgcc ccgttggcga ttttttgtaa aggcaaccag
    18361 tgtggtaacg catcagcccc ctccagttcc gtctgtttaa tctgaaaaca gggcgtttca
    18421 acaggaaaat taatcttacg ggtaaaagag ccaggtgcag aaaggagaac atcaggggct
    18481 gaaggcgtca gttgttgctc aagggcttcc tgacgctgct gctgaatgat aaatggggca
    18541 tttccgttat gccgggtatc agcagcctga agatatgaag gcaataataa agcaggtatc
    18601 agtgtcagtg ataaaatgaa cggacagcgc cggaatacgc gttgctcttc agataccacg
    18661 cctctgcgac ataattttcc agccaaacga aaaacctttt atgcatgctg tgtcaacaat
    18721 cacctgtcag cctcctgacg atgtctgccg ccattattgg caataacaca ctaccgccct
    18781 tccggaattc cccgtatcaa ccggtaccag aatgctgagt aaccagtcag ccaaatcagg
    18841 ctgttttaat tttgctcatt atagaataat gatctgaaaa atatcaatat aacaggtgta
    18901 tcgatcgtaa ttgcagatgc ttgcagatct ttatttcagt atatattgtt aagctggaat
    18961 atttagaata tatatatcca tgactaatgt ggaaatacag aaaattattc cagtgaatgc
    19021 tttttatacg gtctcctggc acagaaaatg gtgatttttg atttgaatca ttgaaatcag
    19081 ttctgctaat aagaagacat gctgaaaagc cgattttcag ggggtgattg aagggattgt
    19141 gagtttttcg ttcggctttt aacgtaactt cctgctttcc tgatattcca cggcaagatt
    19201 aatttttagc ggagcagaat gaataaaatc ttcatctggt gtttttgttg cgcgtaaatg
    19261 caggtgtaac tgtggatgtt atccactgaa gagctgctga tgaggatgaa gacatgctga
    19321 ctaaaaaaat gttacttgtt tgtgcttcgt gtgttgcgct gagtgcctgt tctgcacagg
    19381 aaattgcaga gctgaacagg cagataagtg acggtgcatt tgctgcgaca aaagtactga
    19441 acgtaggtgt aaaccaggca gatgatgatc ttaaatatct gtctcaggca ccccgggcaa
    19501 aaagagagaa acgtgatcat gtcatagaag tccctgttga tgtggataca gctgcgggaa
    19561 gactgaaacg gtactataag ttttattcaa atgaagaagt cgatgcgatt cgtaataacg
    19621 gacatgcgaa cgggaagtgg gccgcagcag caataagtca gaacggacat atgtgggatg
    19681 ccatgcccgg tagttattat aaaatgggta gtgactggga tggtggcgaa tttgatgatc
    19741 atctgacgct tgaacttgaa aaaaatggca aaggaagtcg gatatatata acgtatgcat
    19801 cttcggcacc ggggcatctg aaagataaat ttattgagcc attagttaaa cgtgtgaagg
    19861 atgtggctga aggccgggcc aggtaacgtg tactatataa tatatcccct ggcatacata
    19921 ctgccagggg atgttgcttt tctgtatgtc attttatgat gatacctttg atattcttat
    19981 agatgtttag gtgaactgtt aatgataaat attgtccgat attatacttt cttgtaagta
    20041 tattttggga aggttatttt tattaaggct ttaaatgttt tattttgttc gctttttctt
    20101 tctctgtttt ttgtttcttg ctattacaac tatcttgtga ttgtttatat gctaaacata
    20161 attctgatgt tttagtacat gtaacatatt gacacattct ttatctgttt gtattttacg
    20221 cgttagcagt gtttttattt tgacattgct gttaacaacc gattgctgga gaggtgaaaa
    20281 tgaataaaat gttcagtcag tggattgatt ttgatgagat gattactccg aagattatca
    20341 aggtaatata ctggtttttt ctttttatta cagtggccgg tggtgttata ggtatgatag
    20401 ccaatccgtt tgtcggactg cttggtctta ttatgggtat tattggtctg aggattgggt
    20461 gtgaaatgat aattcttctt tttaatattt ataagcaggt taaaaagatt gctgatgcgg
    20521 taccggataa aataaacgaa caggctgagg atagtttatt atctcagaat aaacattgat
    20581 gaatggtaat aacagggact gttaatactg tccctgttat tttttctgca gactgttttc
    20641 tgttcttcct ggttatgttc tgagtgatga ttgacgcctt ttatacgctc tgatatgctc
    20701 agaaagcatt atctgtcgct ttggcgacca ccaatgctcc ataagtggtc tgagaggagc
    20761 gccttcgggt gaccacatcc cgcttcggtg ggcaccaatg ttccgttgat ctgagaggaa
    20821 cgccttcggg tgatcaacac cccgtaacct gaaaggggat gtgcagccag gatgtttctg
    20881 gtggtggtaa atctgcccac ctttgagagc agatatcgtc agtcatgatg atgaaggttt
    20941 cttttaaccg cgccctttat tacgggcaat acacagcagt ggttgtgtga tccttatcta
    21001 tccggtgacg ccggaatgtt tttactgcgt aatggcggta ttgacttctt ttattctttt
    21061 catttgtttg ccagcacttc ctgcgactgt gcgcgctgta taagaccatt tgaaatttgg
    21121 tcgcttacgc gtgtacctgg cgtctgaagc tgcgcggcgt cactttcgtc agcgtgtcac
    21181 gatgacaaag tgacgcacgc gcgtctgctt aacgaacccg cacgaactgc cgtttcacgt
    21241 gatgtttttt aacatttgaa gaagtgaccc ggcgtgagag tgttttttca acctcttctg
    21301 caaatttacg gacgattttg tgaataacgt ccataaatgg ttttttgcag aatcaattgt
    21361 tttttaagga gttttatgta gtgagatgtc gaatcggcga taccacttat tagaggtggt
    21421 gtcgggggcg acctggagac aggtacagca aatgctgcag tcggtgtgca acaccgattt
    21481 gttacccctc aatacctaaa caatctgcgc taccctggcg cacaggcagt cactacccga
    21541 aggatcgcaa cgtttaaccg tgtcgtcagg gagaaatgca gatgagtttt tctgttgtgt
    21601 tcacggcaca acagaaaaac tcatctgccg aacgtacagc tgtttaaggt aaaaattatt
    21661 atgtgcagga gaagttgatg tccagacgtg aaaaattact gaaacaggat ctggttgctc
    21721 acattcgaaa agacaaaggc agcttcaaga agctgagtga ttacaccagg atcatgcacc
    21781 gctttgctga aagtctggca aaaatgaatg tacagatcag ttctgccgcg caggtaaaag
    21841 tccggcatat tgagctatat atgagaagcc ggagtaatgt ttctgggcga acaagggaaa
    21901 atgaaatgtc tgcaatacgg gttatgttac gtcaggcagg taaatataag atggctgatc
    21961 ccgaacatcc tcgtctcagc aataaagcgt taggcatttc cggctccagt cgtaagggaa
    22021 caaaaaaatt aatcacggat gagcgttttc gggagattct tcagcgtgtg gaagcaaaag
    22081 ataaaggggt ggcagctgtt gttaaactgt cacgttacct cggtttgaga aatgaagaag
    22141 ctatccagtc ggccaaatca ttacgaactt ggaaaaaagc gttacaacgg ggagatgaaa
    22201 aaataagagt gatttttggt acgaaagggg gacgggaaag aatgacaacg gttgttgacc
    22261 gggataacgt tcttgatgct gttaattatg cactcaaata tgccagtgaa cataatggac
    22321 gtcttataga cagagataat cttaagtcgg caatggatta ttatcttaat acattacgtc
    22381 gttatggaga actgaaaggc ggagatgaga ctgcacacag ttttcggtac agttacgctg
    22441 caagtgccat gaaatatcat attatgaacg gttacagtta taaggaggca cttgcactga
    22501 catcaattga tcttggtcat ggtgatggca gaggacgata tattgaacag gtttattgtc
    22561 agttgttagg ctatgagtaa taactcatgg cgatttcgta cgggtaaata ttatggattg
    22621 tactattttg ctgaaaataa tctctgtaaa agagattaca aaaaattcgc ctttgtggtc
    22681 acactatctg gcgcgatatg ttgattccgg atatcaggcc gagtttgaat gtgaaggaac
    22741 agcagataaa tgggatatct atgatgaatg ggacatctat gaatccattg cggattattt
    22801 tgatgtggtg ggagatttta actgtgatgt cagatggtcg ggaagaaaaa tactgattaa
    22861 agggacctgt gtttttcctc tgggagaacc cgttaaggga atacataata ttcctctttg
    22921 gattgatgtt gaataacttg acattcatct tttgtgattt aaaaatatac aggcttatat
    22981 gtcgcttcgg cgaccaccaa tgctccacaa gtggcctgag aggagcgcct tcgggtggct
    23041 acatgtcgct tcggcgacca ccaatgctcc ataagtggtc tgagaggagc gccttcgggt
    23101 gaccacatcc cgcttcggtg ggcaccaatg ttccgttgat ctgagaggaa cgccttcggg
    23161 tgatcaacac ccccgtaacc tgaaagggga tgtgcagcta ggatgtttct ggtggtggta
    23221 aatctgccca cctttgagag cagatatcgt cagtcatgac gatgaaggtt tctttataac
    23281 tgcgcccttt attaacgggc tttttctgac agcaacacca tgctgtactg gtggtgtttt
    23341 aaccctgacg ggagtttttg tccgtcgggg cgaaggctcc cgttttttta caggagtctt
    23401 tatgtcaacg tttattttgt accctgaacc gggcatctct tcttttttcc ggcgaatgac
    23461 ggctcagttg cgggtgtttt tttcccgcaa taacctgtca tattacagtc gctggtacac
    23521 taatccggcc ggcaatctga gcgtgcgggt ctgtatgttc cgtcgtggcg gtgtctggac
    23581 accctgcctg tattaccggc agggaatgta tcgcaacgag tacacaggat ggtatcagcc
    23641 gttaccgcgt gaggtcattt atctgaaccg gtttaccgga cggatatccc gtcgcaggac
    23701 atggcatgct gcctgccgta tggcggagca actgaccacc ctgtgttatc ggtttggtct
    23761 gcagggtgat ggaatcgtcg cacagccctg ctgatttatc atttattacc ctgtcgggaa
    23821 cacgcttccc gcagggcagg tgtgttcccg ttattttttg tatctgaggt gaacacatgc
    23881 atagtctgtt aatactcctt tacgttgttg tatggcgtgc ctgcatcatt cttcgggtaa
    23941 tcgcaccagt gtcggctgtc cgtgcgtggt gtgagtatca actggaaggt atgacgatac
    24001 ttcgtgatgc gtacagctcg ctggctaagg cggctggaat gcagaatgat gcatttgaat
    24061 atactgaaag tattgcacgt catctgggct acaaaagcgg tggtgaccaa ctgaaatcag
    24121 gacttttccg gccgtttctg tggcatatac gtgtgaaagg ccacaggctg ggggctgtca
    24181 tggagatgga ggcatggaag acatggaaga atggctgtaa cgatgtctgc aatccatggg
    24241 aggatgtctg caatccatgg gagcgtggat ggaacgatgg ctggacggaa tgtctgaatc
    24301 aacgtatagc cgggctcctg aagcaggcct gtattgaatt ttccgacgca gactgacggg
    24361 caggatgcca gtcagtcaca ggaaggatgt caaatcgtga gcgatgcaac tgccattttg
    24421 caatgatggc tgttttgctc ccgtaacggt ttttctttta cttactgcac cgtgtttttg
    24481 cgccggtgtt ttccctgacg ggagttttgt tccgtcaggg acaggacttc ctttaagaga
    24541 cggaggtcat catgtattca cctttaattg ctcattattc agcgcttcag acgcagtctg
    24601 ctctgctggc tcatatcagt cagatggaag gcgagctgat gcggttacga gcctggcaac
    24661 ggctgggtat taccccggag ccggatgatg agacggagcc ttcactggta tacgtgtatc
    24721 tgtgggatac cggcgaggcg ctgggctggc tgttgtatga cctggagcag gaggagattt
    24781 ccgcaccggg aattcaggcc cgtcaggcac tcgacagtct gatggctctg gggcgtgaaa
    24841 ttaatcatga aatctgggct gacagtatca gtaccggaaa gatggtggcc ggagcggatg
    24901 aatattttgc ccggcacgac atgatgtaat tcatgacctg accctgacgg gggagttctc
    24961 cgtccgggag acattccccg ttgtttcgtt ttaaccatga ggaatgttcc atgtctgagt
    25021 tatcttcacg tccggcccgt gagccggtgg tatataccct tgagcaggtt tccacaatac
    25081 cggaaaagca gtggcatgct tttgttctgg ctgccactga aaccttctgg cagttgccgg
    25141 aggcgttacg gccgcagaat gcgtatttcg gctccctgac ccgggcgtca gagttgtttc
    25201 cggtaacgga cacgctggcg ttttactgcc ggtctgccga cgggctgtgg tcggtgaatg
    25261 tcactattga gcgggagcac agccggaata ttctggctct taatgagctg aatttcggac
    25321 gtcagccggg agatttcttt gcccgtacag tctttgtgct gttgcacaat ctgtgtccgg
    25381 actgttttcg tattcattcc actgtcggtg gtgccagctg gtcgctgccg ctgaaatgga
    25441 taaaacggta cctggggcat gagaattttt ctgctccgga gtctgtatta accaccctgg
    25501 taaggggaga tgtgtttgac agcttactgc tgcagttttt gtccggtcag gggcgacagt
    25561 tatccccgga tgactgggca gcactggaag aggccgaata tcagctttac tggctgcggg
    25621 ctcttgccgg tgggcggtaa cagaacgccg tagtttcttt atccggaata acgtcatcac
    25681 gtattccgtc attatatcac ccggcaggga catatccctg tggggagagt cccggcttta
    25741 ttcaggagaa ttgttatggg actcgatatt tactttctgg ctcgtgataa ccatcacagt
    25801 ggtgccgttg tgggaacaca ggaacatcag gaggtggggg ctttccgtaa ggtgaaacct
    25861 cttctgacat ggtttcgtga acataccggt gcttcagatg agcagcatgc tttccctgta
    25921 acccgcagtt cactggctgc attattgcgc gacctcagta tgctgacacc ggggaactgc
    25981 cacaggctgt ttccttatgt cacaggctcg tgtttacggg gggaagatta tgccggtgat
    26041 tactggtatg acgtggatga cgtaaaagcg tgggtcagca atacgctgaa gacgtttgat
    26101 tttgagcgtc gttcactggt gatgctggcg ttgtggtaat tctggccagt ctgattttcg
    26161 ttattaattt aactgtacgg tgcactctca ccgtacaggg agcgtgcatc tttttcaggg
    26221 ggcatgctct tatgaaaacg aaaaaacata cttcacgtac cgcgattaaa actccggaac
    26281 gtgacctgta tcagcttgtg accgacaggg ttgttacggc cattgaaaat ggtgtgccac
    26341 cctggaaacg tccctggcgc tctgcgcgta atggcgtggg gcttccggag atgatgccgg
    26401 caaatgccct gaccgggaag ccttatacag gggtgaacgt cctgttgctg tggctggccg
    26461 cggatgaagc cgggtatgtc agcaatgact ggctgacgta ccgtcaggcg caacaactgg
    26521 gtggccacgt ccgtaaaggg gaggtggcca cgctggcggt gatttacaaa gactggaaaa
    26581 aacaggctgt ggatgaacac ggtaaccggc tgacggacag tgagggggag cctctcatgg
    26641 aaatcgtacc ggtactgaaa tcgaatcctc tgtttaatgt ggggcagtgt gacggtttac
    26701 ctgaacatct gatggtgaag gcggaaggca gcgcggatgc actgccggca gataccttca
    26761 gtgagcgggt gctgcaggta ctgaatggca gcggcgtcag cgtctcgtca cttccacagg
    26821 acagggcatt ctaccggcct gatcttgacc gtatcgtgat gccactgtcg tcgcagtttg
    26881 accaggtggc tgattactgg accaccctgc tgcatgagct ggtacacagt accggtcatg
    26941 aaaagcgcct gcggcgggtg gggataagta cctttaccgg tcgcaacagc ccggaatatg
    27001 cgtttgaaga gctgattgcc gagatgggca gtgcttttct gtgtgcacat ctcgggattg
    27061 ccggtgacgt acagcacgaa agttatattg acggatggct taaggttctg aagtctgaca
    27121 aaaaagcgct gtttcgcgcc tgccggcagg cacgtgaagc atcagagtat ttactgacgc
    27181 ggcgaacttc gcaggagtgc gcaacataat ttttactacc ggtggcggtt ataacctgac
    27241 cgccatttat cccgtgaggg cattgtacct cacgggacaa tgccctcttt ttacatctgt
    27301 ataaaaaaga ggagcgtaaa caatgtctga atggtgcaaa aaccgttttg aaattaccgg
    27361 taaatcagtc tgtcttgatg tacttacaca gtggattgaa gggcgtgaga ccccgcgcta
    27421 ccgtcatgcc atacagcaga gtatacagct gtttctggcc ggttgtgcag ggctggtaaa
    27481 acccacacgg acgacgcagt atccgccata tccggcgctg gttcgtcacg gaacggggtt
    27541 gtcatctccg gctaaccagg cgtttgagca gtggctgggg ttgctggtaa aaaatgccgt
    27601 tctggatggt gaaacgataa agaccattga gcgtctttat caccagtcat gtatcggcgc
    27661 tgttcgctgg ggtaatctgc cggataatgc cagggagatc atcaccacgc tgatgagccg
    27721 tcagtattct gactggtttg gtctggccgg gctgagtgaa cacattgatg cagaagcctg
    27781 ctggagctgg ttgtcggatt acccggaaca ggcgcagccc tgtgacatgc tgatggtcat
    27841 accctcccgt ctggctacag agctgaatgg ttcaggtgga ctgctgcaag gtatttccac
    27901 aacgaccagc ctttacagcc ggatatacgg tgtggagtgg ccgtcaggaa ataatgttcg
    27961 ccatgcgcgt gatgagatga gcagtctggt gctgctgatc gacacgccct ggtatccgcc
    28021 ttcaggcgaa ctggttggtg agatctcgaa ggtgtttgac tgcgaggtcc gacactggta
    28081 cagcgagccg gtacacggta tacagggtta caactgttac gatggcggag agcatacgga
    28141 cagtgatctg caggccgaat ggccgggcag ggaaacactt ccacagcccc gactgtatct
    28201 ggtggaggag tgcgctgaag aacagacgaa agccgcacct ttaccggttc ctctggtctc
    28261 agcccagtaa ttaacctccg ggtatcatgt ccgtgatgcc cgttttatca acatttcaac
    28321 ccgaagggga acacctgttc ccctgtccgg gccatgtgtt ccctgttttt acggagaaaa
    28381 catggccacc atgactgacc atcagaagga gttcatttcg ttgtttaacc ggacagcctg
    28441 ttatcacaaa cgtcataagg tgtttgaaga ctttgtcagc tgtagtgtga ttgccctgga
    28501 aaaccggctt cagttcagtg aggcccggga acagaagtat ctgcgaatcg tcagtggtta
    28561 tgaaaagcag gatgttatcc acattgcgca tttgcttgct catgtcattg agggactgga
    28621 gcagggattc tgtgattttc tgggtagtgt gttcatgcaa ctggaactgg gggataaata
    28681 ccggagtcag tttttcacgc ccaggagtgt tgccagcatg atggcacaga tgcaactggg
    28741 aaatgtgaag gtgttgtttg agagtaaacc ctttatcacg ctcagtgaac ctgcctgtgg
    28801 tgccggcagt atgattctgg caatggctga catcctgaac cgttcggggt atccggcata
    28861 ccacaggatg tgggtgtcgg caacggatat tgacccgctg gcagccggca tggcgtatat
    28921 acaactgtcg ctgtgtggtg tggccggaga agtggtgatt ggtaactcgc tctgtaatga
    28981 gcgacgccgc gtactgctga ctcccgggca ttacctggga aactgggacc atcgtctgcg
    29041 tcatcttcag gagcagaatg tggcatgatg cagaatgttt tatcactgcg ccagtcttcg
    29101 gactggcgtt ttgttcaact gccagttccc tgaatgtttg atttggattt gttcaataaa
    29161 gcctgtttat caataaattc tctgacgaca gggaatggta tgtttttatt agtttttatg
    29221 tgttggacaa caagacagaa taatgatgtc tgttttatgt atacgatata gccactgatt
    29281 caacgcatta tcggctatat tgccagtctt atttagtgcc agattcattg tatcgaaggc
    29341 cgctgccgtc agtagatggc tggtcagttc tatcagacag accattttta cctgaggata
    29401 aagtgtctgg tttccaccat gtatttggcg agtgaagctt tatcgtgttc ttgcgtgtcc
    29461 tgtgttcgtc agaacacgcc atcaatggtc agcagattca gatcgcacca gagcggatat
    29521 ggcgtggtgt tatgctagag ctgcgctgtt tgagtgaaca cgcagcgtat ggctccgctt
    29581 cctggccgct ggcgggcatg gagcacggcg ctactgcctg gcagcatgat gtccagccgg
    29641 ttcacaatct gatgaaggtt ccttacgctc atgggccata ctgataattc accagaccat
    29701 catttccaga ggaagacgcc gtttgtgtag cgtgacagtg tcggactctt cagggctgtg
    29761 ggtaatcagt tccagggcaa gatagtcgca gagggttacg cagagaatag taactggaca
    29821 ctggatcgat caaccgatcc ttaactgagc ggcatgacag ctttctgatg gctttttttt
    29881 gataatcttc acattgtgct tcttttctgt tggataatct ttgtacttga tggtgtacta
    29941 ggtatcccat tatgcagttg agtgaaggtg attaaaaatg caatgtgaat ttaaaggggt
    30001 catatcagca ttacccactc cttatgacca atcccagcaa atcgatatgg agagtcttcg
    30061 aaaactgatc cgttttaata tcgaacaaaa tattaaggga ttgtatgttg gaggctctac
    30121 tggagaagca tttcttcaga atgttgcaga gcgagaaaaa attcttgaaa cagtagccga
    30181 tgaatcagat ggaagactaa cacttatagc gcatgtgggc ggaatcagta cggcagaaag
    30241 cgaggtactt gcaaaagctg caaaaaaata cggatatcat gctatatctg ctgttactcc
    30301 attttattat cctttttcat ttgaagagca ttgtatccat tacaggaaga ttattgattc
    30361 tgcagatgga cttccgatgg tagtttataa tataccggct cttagcggag tgcgtttttc
    30421 tttagatcag ataaatgagc ttgtcacaat acccggggtt tgtgcattaa aacagacatc
    30481 cggtgattta ttccagatgg aacaaatcaa aaggaatcat ccagagcttg tgttatataa
    30541 tggttacgat gaaatatttg catcagggtt aattgcaggt gcagatgggg gaatcggtag
    30601 tacatataat ataatggggt ggcgttatct tgaaatattt gaagcagtca aaaataatga
    30661 tgtgattaag gcgaaggaaa tgcaggtggc ctgtaaccag gttattgata ctcttattca
    30721 gagtggtgtc ctggcaggta taaaaactct tctttactat atggggatta taaatactcc
    30781 tgtatgcaga agtccttttt ctccggtaaa ggaaaagaat ctggatgtat tatcaaaact
    30841 tgcagagcga ctgctcgaag aacatgacag aaataaaaaa atgaaaataa tctgaataat
    30901 tgcaagattg ttatataggt aaaatgaatg ataacactgg ctgttgatat aggaggaacg
    30961 aaaatttcag cggcgttgat atctgatgac ggttcatttt tgctgaaaaa acagatatca
    31021 acacctcatg aaagatgtcc tgacgaaatg acaggggctt tacgtctact ggtttctgaa
    31081 atgaagggaa cagcggagcg atttgcagta gcatcaacag ggattattaa taatggagta
    31141 ttgacagctt tgaatccaga caatcttgga gggctgaaag agtatcctct gaaaaatata
    31201 atggaagata taactggttt aaatggaagt gtgataaatg atgcccaggc aggcgcatgg
    31261 gcagaatata cagttttgcc aaaagaaatt tgtgatatgg tttttataac cgtttctacg
    31321 ggggggggag ggggaattgt agttaacaga aaattattaa caggtgtttc tggattagcc
    31381 ggacatgttg ggcatatatt gtccggagta acggatacag aatgtggatg tggtcgtcgg
    31441 ggatgcgttg aagcggtttc atctggtggg ccatcatggg ggcagcaaaa aatgaacttg
    31501 caggatatag caccaaatat atttttgaat tagcgagaca gggatataag gaagccgaat
    31561 ttttgacaga gcgttctgcc agtactattg ctgaattaat agtaagttta aaattactgc
    31621 ttgattgtca ggttgttgtt gtcggaggaa gtgttggact tgctgatggg tatattcaga
    31681 aagtgtcgaa acatctttcc atatattctg aaatttgtaa tgtaatgctg tttccggcat
    31741 atttcagaag tgactctggt cttattggtg caactctttg ggaccgggat tgtattactt
    31801 aaagtaatac tccagtgtat tataaatccg ctattattgt gaggtttaat atggactcct
    31861 gtattcagaa aaagcaggtt tggtataagc atctttcacc cagacagtgg aagatattcg
    31921 gtgcggcatg gacaggttat ttacttgatg gttttgattt cgttctgata tctctggttc
    31981 ttacagaggt tcagcatgaa ttcggcttga cgacaataga agcggcaagc cttatttccg
    32041 ctgcttttat ttcccgatgg tttggggggc ttgctattgg cgctttatcg gacaagatgg
    32101 ggcgcaggat ggcaatggtt ttaagtatag tgctattctc tctcggaacc cttgcctgtg
    32161 gtctggctcc tggatatgca gtgatgttta ttgctcgcat tgttattggg cttggtatgg
    32221 caggtgaata tggctcaagt gtaacatatg ttatcgaaag ctggcctgtt catctcagaa
    32281 acaaggcaag tggtttttta atttctggat tttctattgg gggaggttta gctgcacagg
    32341 tttatagcat tgtagtacca ctatggggct ggcgttctct tttctttgtc ggcatgcttc
    32401 caatattatt tgctttttat cttcgcaaga atcttccgga gtctgatgac tggcaaaaaa
    32461 ggcaacaaga gaataaaccc gtcagaacaa tggtggatat tctgtacaga gaaaagaata
    32521 aatacataaa tattcttctg tcttgtattg catttgcatg tctatatgtt tgtttttcag
    32581 gcgtcactgc aaacgctgct ttaatcacag ttatggcatt gtgctgtgcg gctgttttta
    32641 tcagttttat atatcagggt atggggaaaa gatggccaac aggcattatg ttgatgcttg
    32701 tggttatgtt ctgtttcctc tacggatggc cactgcaggc ttttcttcct acatggctta
    32761 aggttgatat gcagtattca ccagaaactg ttgctctcat atttatgctg gcaggttttg
    32821 gctctgcagc tggaagttgt ataggtggtt ttatggggga ctggcttgga acccgaaaag
    32881 catatgttat atcacttctt atcggtcagc ttgttattat ccccgttttt cttgtggacc
    32941 gggactatgt atggttactt ggccttctga tctttactca gcaggttttt ggtcagggga
    33001 ttggtgctct ggttccgaaa ataatcagtg gttattttaa tgtggagcag cgtgcggcag
    33061 gactggggtt tatttataat gtaggttcac ttggtggagc atgtgcgcct attcttggtg
    33121 cagttgttgc gtctcataca agtcttggta cagcaatgtg ttctcttgct tttatcttaa
    33181 catttgttgt tcttgtcctt attggttttg atatgccatc acgtgtacag agatggatcc
    33241 atccagaagc tgcgcttgaa tatgatactg tcgatggaaa acctttttat ggagcaagaa
    33301 aaaagaatgt tgcaggagaa taaaggatgc tatcagggaa taaaaaaatg attcattaca
    33361 gggagaatat atattctctt gataattatg agcattacat aactgacaat tcgctatttg
    33421 agaatactga cgatggcata atatatacag gtattgtgcc ccttattaca gttgttgagc
    33481 ctgttgtatc taatcaacat agtgtgctga ttatacctgg aggtggatac aggaggattg
    33541 tttatgaaag agagggactt gatgtaggca gatggatagc aggtcaggga tatacggcat
    33601 ttattctttc ttacagaatg ccctccgtta atggttatta ttcctttgcg gatgcgaaga
    33661 atgccctgaa ctttatttct tactgggccg gaatgtcgga gagaacattt aagagtgttg
    33721 gatgtatggg gttttcggca ggtggtcatc ttgctggata tattactcat ctccattatg
    33781 aggaagagac ttctgtgcgt cctgactgga tggctctgat atatccagtt atatctcttg
    33841 acaggagtat tgctcatcag ggatgccgga aacgtcttct ggatgcaggt gaaagtgaat
    33901 attcagccag tcttgaaaat agtgttacag agtatatgcc accagtcttt attgtgcatg
    33961 caacagatga taaggatgtt ccagttattc actctctgag atattttaac tctatccggg
    34021 ataagggaca tcatgcagaa gctcattttt ttgataaggg agggcatgga tttggtgtat
    34081 ccggaaaaaa tggatatccg atagaaaaat ggcaaagcct gcttctggaa tggcttgcgg
    34141 aacataagtt tcattgagaa atcagattac aagtgataaa tatatttaat gatgaggcat
    34201 ttatgaaaaa atattatcca ctattaactg tgattcttct tggtttacct ttgtgctctc
    34261 aggcattaac tcttaatcta cgtggaggat acagaagcgc cagtcacgct tatgaaacgt
    34321 tgattcaggt tagtcagggg tggaataatg gttggtgggc aagttttgag tcaaatacct
    34381 ggaatacaat tcacaacaat gaaaatgaat tgtttgcact aaattataat aaaatagata
    34441 taaataaaac atataaaata gatgacaaat gggctctaat cccagggatt cagacacaat
    34501 ggatgtcagc aggaagtcag tataaccctt atctgaaaat ttcgtatcag gcaacgccag
    34561 atcttaatct ggccttacgc tatcgctatg actggaaagc tttccgacag acggatttag
    34621 atggcaacag ggcgcgtaat gatcagcatc agtttgatgg ttatgtgaca tacaaaatta
    34681 atgattcatg gttgtttgca tggcagacca cggtatatac gaaggttaat aatttcaaat
    34741 atgggaatca taaaaaaaca gcaactgaaa atgcatttgt tctgcaatat aaaatgtcac
    34801 cggtgtttac cccatatata gaatatgatt acctggataa acaaggggtg tataaaggga
    34861 aagataataa acatgaaaat agttaccgtg tcggtgttct gattaatttt taaaaggtgg
    34921 ttataatgaa agtgaaaaac tttatatatc ttcccttctg cttgtttatt ggaacgtcgg
    34981 tagccggagc tttaccggaa attcctgaac catttaaata tggtgtagga gggatcgata
    35041 atggtaaaat atatatagga ctgggaagtc tgggaaataa ctggtatatg atagatacaa
    35101 accagagtga aaaaaaatgg actaagatag cacaatggcc gacagttccc agagagcagg
    35161 caacagctac tattattgat ggtaagatct atgtatttgg aggaattgga aaagataaaa
    35221 gtggagtcat aaccttacaa aaagatgtct attcctatga tattgctaaa gataaatggg
    35281 aaaaattgat gacccgtccc cctgtatctc ttgccggtca tgtttctttt atccataatg
    35341 gtcatgcggt atctactggt ggtgtaaatg agaatatttt taatggatac ttttcagatg
    35401 tcgagttatc aaaagggaat tccgcgctga cagagaaagt caacagggat tattttagta
    35461 aacctgttga tgattatttt ttaaataatc atatgatttc ttatgacccg tcaaaaaacc
    35521 agtggaagaa cctgggaacg actccatttc cgggaactgc tggttcatcg gtgatttttg
    35581 cagaacagca aatttatata ctgggagggg agagaaaacc gggccttcgt tctgttagat
    35641 cctggacggg ggagctttcg catgatcgaa ttaaatggtc tgaacttcca ccagtagctt
    35701 cacccgaggg ggtgtcagga gcatatgcct ctgttattga tggaaatatc tttctggccg
    35761 gtggtgcata tttcccaggg gcggcagaaa agtatagcaa tggtgaatac tggtctcata
    35821 aaggactgga taaagcctat tcaaaagaga tatatcagct aataaaaaat gactggaaaa
    35881 aagttggttc tttgccggaa ggacttgctt atggtgtaag cctgccctgg caggagggga
    35941 tgctcattct gggtggggag aaaaaaggtg gtaaagctgt ttcagatgtt atttatctga
    36001 aaaaaaatga caaacaaata aagattgtta aataactgta atgcaggtga ctgtattagt
    36061 tgttcagtca cctgagttat tttaatggag tttataatga ataatgataa atctttaaat
    36121 gaaatgatgt taagcttgtt taataaatca actattgttg acttgtcaag tgagacaata
    36181 tctgagagta aatctctttc aggccgtatt tatcaggatc ctgagcataa cggggaattt
    36241 gaacttgatc gtgtattgac agatatttat gctcctcagt taattatttt tttaccagat
    36301 aaaccaaatg gaacaggaat attgattatt cccggggggg gctaccgggc tgttatgtta
    36361 gataaggaag gtacagctct tgctccagag tttttatctc agggctatac at
//
LOCUS       DAZGSP010000047        35595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZGSP010000047 DAZGSP010000000
VERSION     DAZGSP010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.047"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(18..93)
                     /locus_tag="ACHK74_002509"
     tRNA            complement(18..93)
                     /locus_tag="ACHK74_002509"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="ACHK74_002510"
     tRNA            complement(152..228)
                     /locus_tag="ACHK74_002510"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /gene="yifK"
                     /locus_tag="ACHK74_002511"
     CDS             complement(331..1716)
                     /gene="yifK"
                     /locus_tag="ACHK74_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0221311.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRYGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="wecG"
                     /locus_tag="ACHK74_002512"
     CDS             complement(1907..2647)
                     /gene="wecG"
                     /locus_tag="ACHK74_002512"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFP0221312.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="ACHK74_002513"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="ACHK74_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFP0221313.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="ACHK74_002514"
                     /gene_synonym="wecF"
     CDS             complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="ACHK74_002514"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFP0221314.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="ACHK74_002515"
     CDS             complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="ACHK74_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFP0221315.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIFGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="ACHK74_002516"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="ACHK74_002516"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFP0221316.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVIST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="ACHK74_002517"
                     /gene_synonym="wecD"
     CDS             complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="ACHK74_002517"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFP0221317.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="ACHK74_002518"
     CDS             complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="ACHK74_002518"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP0221318.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="ACHK74_002519"
     CDS             complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="ACHK74_002519"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP0221319.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="ACHK74_002520"
     CDS             complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="ACHK74_002520"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFP0221320.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="ACHK74_002521"
     CDS             complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="ACHK74_002521"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFP0221321.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="ACHK74_002522"
     CDS             complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="ACHK74_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFP0221322.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="ACHK74_002523"
     CDS             complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="ACHK74_002523"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFP0221323.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14927..16186)
                     /gene="rho"
                     /locus_tag="ACHK74_002524"
     CDS             complement(14927..16186)
                     /gene="rho"
                     /locus_tag="ACHK74_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFP0221324.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="ACHK74_002525"
     CDS             complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="ACHK74_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFP0221325.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="ACHK74_002526"
     CDS             complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="ACHK74_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFP0221326.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16973..18238
                     /gene="rhlB"
                     /locus_tag="ACHK74_002527"
     CDS             16973..18238
                     /gene="rhlB"
                     /locus_tag="ACHK74_002527"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFP0221327.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKYRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18372..19856
                     /gene="gppA"
                     /locus_tag="ACHK74_002528"
     CDS             18372..19856
                     /gene="gppA"
                     /locus_tag="ACHK74_002528"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFP0221328.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(19903..21924)
                     /gene="rep"
                     /locus_tag="ACHK74_002529"
     CDS             complement(19903..21924)
                     /gene="rep"
                     /locus_tag="ACHK74_002529"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFP0221329.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22141..22590
                     /locus_tag="ACHK74_002530"
     CDS             22141..22590
                     /locus_tag="ACHK74_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP0221330.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            22789..23070
                     /gene="ppiC"
                     /locus_tag="ACHK74_002531"
     CDS             22789..23070
                     /gene="ppiC"
                     /locus_tag="ACHK74_002531"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFP0221331.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(23157..24632)
                     /gene="ilvC"
                     /locus_tag="ACHK74_002532"
     CDS             complement(23157..24632)
                     /gene="ilvC"
                     /locus_tag="ACHK74_002532"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFP0221332.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            24782..25672
                     /gene="ilvY"
                     /locus_tag="ACHK74_002533"
     CDS             24782..25672
                     /gene="ilvY"
                     /locus_tag="ACHK74_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFP0221333.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QALFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(25669..27213)
                     /gene="ilvA"
                     /locus_tag="ACHK74_002534"
     CDS             complement(25669..27213)
                     /gene="ilvA"
                     /locus_tag="ACHK74_002534"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFP0221334.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(27216..29066)
                     /gene="ilvD"
                     /locus_tag="ACHK74_002535"
     CDS             complement(27216..29066)
                     /gene="ilvD"
                     /locus_tag="ACHK74_002535"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFP0221335.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(29131..30060)
                     /gene="ilvE"
                     /locus_tag="ACHK74_002536"
     CDS             complement(29131..30060)
                     /gene="ilvE"
                     /locus_tag="ACHK74_002536"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFP0221336.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(30080..30343)
                     /gene="ilvM"
                     /locus_tag="ACHK74_002537"
     CDS             complement(30080..30343)
                     /gene="ilvM"
                     /locus_tag="ACHK74_002537"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFP0221337.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(30340..31986)
                     /gene="ilvG"
                     /locus_tag="ACHK74_002538"
     CDS             complement(30340..31986)
                     /gene="ilvG"
                     /locus_tag="ACHK74_002538"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFP0221338.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31989..32039)
                     /gene="ilvX"
                     /locus_tag="ACHK74_002539"
     CDS             complement(31989..32039)
                     /gene="ilvX"
                     /locus_tag="ACHK74_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFP0221339.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(32126..32224)
                     /gene="ilvL"
                     /locus_tag="ACHK74_002540"
     CDS             complement(32126..32224)
                     /gene="ilvL"
                     /locus_tag="ACHK74_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFP0221340.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            32577..34097
                     /gene="yifB"
                     /locus_tag="ACHK74_002541"
     CDS             32577..34097
                     /gene="yifB"
                     /locus_tag="ACHK74_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFP0221341.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLENEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(34122..34460)
                     /gene="maoP"
                     /locus_tag="ACHK74_002542"
     CDS             complement(34122..34460)
                     /gene="maoP"
                     /locus_tag="ACHK74_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFP0221342.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            34579..35418
                     /gene="hdfR"
                     /locus_tag="ACHK74_002543"
     CDS             34579..35418
                     /gene="hdfR"
                     /locus_tag="ACHK74_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFP0221343.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNIFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDELSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(35514..35589)
                     /locus_tag="ACHK74_002544"
     tRNA            complement(35514..35589)
                     /locus_tag="ACHK74_002544"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35554..35556),aa:Trp,seq:cca)
BASE COUNT         8704 a         9689 c         8993 g         8209 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ccgggactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccccccc tctgtctagt ccctttttaa ataaattgcg
      301 cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg ctttcccgtg
      361 gcgattaagg ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccaac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
      721 gggtacgccg taacgggaaa ctttcgccat cgccgccggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
      901 tacgaacggg ctgccgttgc tgcctatttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggatccg ccacagcacc ttgcctacgg cgctgcgcag
     1021 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gcgcggtcag gaaccctttc cagccacccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgcac cgccgccaga ttcgccaacg ccaccagcgc
     1321 cactgcgatc aatgcgggta tccactgcgc catctccggg aaccagaact ggacataaac
     1381 gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccaat aagaccaggc
     1441 ggtgagataa ccaaagaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaga cgaatagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcaaggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 acaggcgacg gagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     1921 tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccaatccca gcgtttgcca gattttcggt gcgcgtttta
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg actgccaacg atattcacat tccattggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaacgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattaactc cctgacctcg gcgttatctt caatggtcag cattttttcg gcattaatgg
     2521 caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcgtgctgca
     2581 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     2821 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagt
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagtc caaccacaat
     2941 cgccccgagt gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agtccggagt ggttattcag cacttcccag gtaaagtagt tggctgagtt
     3061 cagcaccata ctcgggcgac ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     3181 attctcccat ggcgagaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     3241 gccgctcaca ttcattccat agcgttttag cgccagccag aacatgccca caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctat cctggcgcag
     3481 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggagaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgatgccc atcaggatca cccacgtaag
     3661 attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cgcctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagaca acaaacaggc cactgaattg cagcagactc atgcgacctc cccggcggca
     4021 atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     4081 ttatccaccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtca
     4141 gtagtaaaca gcaccggcaa atgctgttcc gtcatatcct gccagaacgg attttcccgg
     4201 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaaa gcgtaccaat gccctgctgg
     4261 cgggcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccag ccccgcctga
     4381 cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     4441 accgtatcgc caaattgctg atgaacggcg cacaaggcag caatatggtc attgctgcgg
     4501 tcaccggagt tccccaccag aatggtcatt ttcccttcac gttgccgatc gttcgccatc
     4561 gtattgagag aagggtccat ccgcgtcggg aagtacagca gttcaccccg cacctttgga
     4621 tgcgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcatac
     4741 aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc actcagcaga
     4801 gccagccaca gtgtgggatt gaactgaccg tggaagaaaa aacgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg
     4981 tgctcgctcg tcgcagccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga
     5041 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     5101 acacgccaca acaaagagaa aaatagacga tataagttgc catatatgcc tgcgccgcgc
     5161 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     5221 tgatttccgc cagaatataa aaccgcaatg acgctttggc gatcaccaga taaccaaaga
     5281 cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcatag
     5341 cggtaaattt attcgacaac agcagccaga tagcaaaatc acgcagcagc cagacggtaa
     5401 aactcgccgc cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga
     5461 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     5521 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacttcat
     5581 cccagctata ctgcgccgcg agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     5641 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     5701 agctgggttt cagatagctt aacgggatga caccacgttt gatcaacatg atggcggcag
     5761 gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca
     5821 aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgaaaatc agcgataacg
     5881 cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg ccccaggcga
     5941 tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta ttaccaaaca
     6001 atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaaac
     6061 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt
     6121 actgggcaac gtatttggtt acaccgttaa agatgccagc tccggcaagc acgccgagca
     6181 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacacag
     6241 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc
     6301 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     6361 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc
     6421 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agcgttcccc cgcagggcaa
     6481 ccgtgcagcg gaatgtaatg gaacaccgcc atgatttccg cttctttcag aaagttaatc
     6541 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
     6601 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg cgctaacgca
     6661 tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt
     6721 tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc aatatcgcgc
     6781 caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc cttttcacgg
     6841 atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc gccttcacca
     6901 cccgccgtat agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
     6961 agtgcacgcc ctttgtaagt ggatatcacg ccctgagcgg catcttctac cacaaacaga
     7021 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
     7081 accggcacga taacgcgcgt tttgtcggtg atcgccgctt caatcagcgt ttcgtcgatg
     7141 ttcatggtgt ccgggcgaac atccacaaaa acgatttttg cgccacgcag cacaaaggca
     7201 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
     7261 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
     7321 ctaccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
     7381 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
     7441 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
     7501 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa
     7561 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctac
     7621 accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac
     7681 ataaccgcga ataccgccgg acgccgcacg taaaatcaga cattgatgat caaaggtgcc
     7741 gcgcacggca ttttcaatcc actgtgcata aaagcgtctg ctggcgtcag gcgcatacca
     7801 cggcgcacga aaacggcttt gcgcaaatgc ggcgctggct aactgacgta atgcgggaat
     7861 atcggtctct tgtgccacta cagcaccact atcactgaca ttgttcacgg gtagtgccaa
     7921 atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca gttcacccgt
     7981 atttgatgcc gcaattttgg cctgcacccg tgaccacggc gctaacacgt ctggcgtcag
     8041 gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc
     8101 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
     8161 cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc
     8221 cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt ttttctactg
     8281 tctgcacaaa ggtactggct tcaatcaggc tgtcgtgagt gccagtgtcc agccaggcaa
     8341 atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc tggttgatgg
     8401 aggtaatctc cagttcacca cgctccgacg gcttcacctg ctttgcgtac tccacgactt
     8461 tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt tttggctttt
     8521 cttccagcga gatagcgcgg aaattgtcgt caaactccac cacgccaaag cgttccgggt
     8581 ccatcacctg atagccaaat accgtcgccc cttccgtgcg cgccgcaaca tgacgcagct
     8641 tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa gaaggttcac
     8701 cgttgaggaa ggtttcaccg atgataaagg cctgcgccag accgtcaggg ctgggctgtt
     8761 cggcgtattc cagctggata ccgaactcac taccatcgcc cagcaggcgc tggaaataac
     8821 ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccagc atcagcaccg
     8881 acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc
     8941 gcgtaatcgg atgcaatcgg gtaccggaac cgcccgccag gataatacct ttcatgtttg
     9001 cctccgctga aaattagcct ttcagaccta aacgctcgcc ctgatagctg ccgtcctgca
     9061 cctgcttcca ccagctttca ttagccagat accactgcac cgttttacgc attccacttt
     9121 caaaggtttc ctgtggcagc caaccaagtt cacgggcaat tttcgaagca tcaatggcat
     9181 aacgcagatc atgccccgga cggtcagcga caaaggtgat caagtcacga tagtgcgcca
     9241 cgccgtgcgg cttgttcgga gccagttctt ccagcagctc gcaaatggtt tccacaacat
     9301 cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact ttcccggtgg
     9361 tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca cggatttgct
     9421 gcccgttgcc atataccggc agcggtttac ccgccagcgc gttgaggatc atcagcggga
     9481 tcagtttttc cggaaagtgg taaggaccgt agttattcga gcagttggtg atcagcgtcg
     9541 gcagaccgta ggtccgcagc caggcgcgca ccagatggtc gctgctggct tttgacgcgg
     9601 aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca
     9661 ggtcaccata tacttcgtcg gtggagatat gatgaaaacg gaacgctgat tttttatctt
     9721 ccgtcagcgt attccagtaa gcccgcgccg cttcaagcaa tgtataagtc ccgacaatgt
     9781 tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt tcggctgcca
     9841 gatgcatgac acagtctggc tgatgctcag tgaatacgcg tgccagttct gcccgatcgc
     9901 agatatcaac tttctcaaag gcaaagtgct cgctttgcgc gaccggtgcc agcgacatca
     9961 ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc atcgctcgtt tcgttgatga
    10021 tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga atttttctca
    10081 tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt cgccattgat
    10141 aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg ccagcgcctc
    10201 gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt ggatgttagg
    10261 ctcaacaacc agcgtttcgc cgctgtgcca ctgggcgatc agttcagcga tttccatcgc
    10321 cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc aggcgatttt
    10381 cagttcactg gcgcgtttat cggtagcagc aaggcagtcg gcaaccgccg ctttcacctg
    10441 atcgataacc cagaacggtt tgtgatcgtt aacttcgcgc gcggtacgga taagccgcgc
    10501 ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt gaccgcccac
    10561 gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc gaatcagttc
    10621 ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa aagcgatatt
    10681 cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg agttagtgac
    10741 gacacactca ccttcgagga aaattttgta cagttcgctg gcgcgggtcg aacaaaccgg
    10801 cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct ggcccggtaa
    10861 cacgcgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct gctgtgggaa
    10921 agtgagatcc ggacgcatct ctgctaacca ttctgccatc ttctcggttg accccaccgg
    10981 cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa tggagcgagc
    11041 agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg taggtacagc
    11101 aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac cgccttctac
    11161 ggcagttttt actacgctcg ccaaatcagg ttcgacgata tggatttcgc cacgattgat
    11221 ggtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc gtgaggcaaa
    11281 cgcagctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg tcgcaaaact
    11341 catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc ctgaccatca
    11401 ccatacgggt tatgggcgcg gctcatagct tgatattcgt tttcgtcttt taaaagacgc
    11461 gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc
    11521 accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc
    11581 gcttcttcct gaatgccgcc tgagtcggtc aaaatcagcc aggcgtggtt catcagccag
    11641 acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg ccccagaatg
    11701 cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat ctggatgtcc
    11761 tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc acgaccgaaa
    11821 ctttcacgcc tgtgaccggt caccagaatc atctttttat cggggtcgat aaacgggtaa
    11881 tttgccgcca gttctgaacg cagcgtgtcg ctgctcatca cctgatcacg cacccataac
    11941 agtgcatcaa tgactgtatt accagtaatg aagattcggc tatccgcaac gttttcacgc
    12001 agcaagtttt gccgggaagt ttcggttgga gagaagtgat acatcgccag atgcccggtc
    12061 aatgtacggt tagcctcttc cggccacggc gaatagagat cgcccgtgcg taaaccagcc
    12121 tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc cagcgtcgtt
    12181 gttgtgtcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat aggttttagc
    12241 ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat tatgttgaga
    12301 tcgtagtcag gtacaatgga aaagagtttc agcacctgat cgagcatctc ccgatgctgc
    12361 gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg caccaacgga
    12421 gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac atcgattctc
    12481 ttcgaataag cggcgagcgc ctttgcgctc gccgcagcag tgttgctatt tcgagcaacg
    12541 gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca taatcatcag
    12601 gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca aatagcgata
    12661 ggtctgaaaa cgcggatcca gagttggacc aacattcagg gtgtttaaca tggcccgatt
    12721 ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca gtcgagcctg
    12781 gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg caggcacatc
    12841 tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct caatgctgtt
    12901 catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct gagctttcat
    12961 ctggatggtg cgtgccgccc atgcgccttt cagctcatca ttcagatggc tggctgcacg
    13021 ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg cggtttcggc
    13081 aataagcttc acgctgtcat tgaccgcgcg ggtaaagtct ccggggataa actggatgtt
    13141 gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca tccgctgttt
    13201 gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca gttgcataac
    13261 aaactcttta taggcttcgt ccatgaccga tggttgatcg gcagaagcca tgtttgaacg
    13321 gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca tattcaccgt
    13381 tggacgatcg gtaatcgccg tcgagctcca ctcctgacga gcaaaaaaag tatacgccag
    13441 cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag cccacaaggt
    13501 acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt tcccaggcat
    13561 tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc acgatttcta
    13621 cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg cttaatgcaa
    13681 tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc cgggacaaaa
    13741 tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag ggtaatcagc
    13801 acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat atgctgacgg
    13861 tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc caccatatcc
    13921 attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg ggttttgccc
    13981 tgggtcgttt cgagcaggat ccagataacg gtaaaaccaa tcagcgtact gcccgcatca
    14041 cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat gtatggcagg
    14101 atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc gaaccacaaa
    14161 atcataccga ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc atcaatgcca
    14221 tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag gaagtaacca
    14281 aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact gagataaagt
    14341 ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg gatttttacg
    14401 ctgatatcaa aacggtcatc cagcgcgcca atgaaaacaa gcacaccggc acaagcgaga
    14461 tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca aatccctgcg
    14521 taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg gaagtttggt
    14581 ttatccacta aaccgacttt ttttgccacc ttacgggcaa aaaacagaaa cagtgtcgtg
    14641 aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat gcatgctctc
    14701 agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg gaaagtccgt
    14761 tccgaaccca ccagtcctgt gcaaaaatta accatttgat atgcaaattg cataacatgc
    14821 cagcaaattc caggaatttt cctaacatcg ccagcgcggc attaagatta cagatataaa
    14881 agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat gagcgtttca
    14941 tcatttcgaa gaaatcgtca ttggtcttgg tcatggccag tttattaatg aggaattcca
    15001 ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt ttctgcagtt
    15061 cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag tcgatagccg
    15121 ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg cctgtacctt
    15181 taaactcttc gtaaataact tcgtccattt tagaaccggt atcgataagc gccgtcgcga
    15241 taatggtcag gctgccgccc tcttccacgt tacgcgccgc accgaagaag cgtttcggac
    15301 gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc ggaacaacag
    15361 tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg tctttcttgt
    15421 gctcaaccag gcgtttggcc ttctcgatca ccatttccgc aacctgaacg tggcgagatg
    15481 cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc atctcggtta
    15541 cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg tggttgtaag
    15601 caatgctctg agcaatgttc tgcagcagca tagttttacc ggctttcggc ggtgccacaa
    15661 tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga gcggttaaat
    15721 cttcagtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc agcggggtta
    15781 agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa
    15841 ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta ccagagatgg
    15901 tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag atgtcatcag
    15961 gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc tgcaatatct
    16021 ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc aggatggcaa
    16081 aaataatgtc ctgcttacgc atacgagcca ggttttccag ccccatattt tcgccgagag
    16141 tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg gtgtgagttc
    16201 ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga tcatccatgc
    16261 ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag tacgcagaac
    16321 tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc atttaagata
    16381 aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt aatacgaagt
    16441 caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa
    16501 catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag
    16561 tgcacccact ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc
    16621 acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt
    16681 cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg
    16741 accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt
    16801 gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat
    16861 aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat
    16921 ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa
    16981 aacacattta acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaagc
    17041 ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac
    17101 gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt
    17161 tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc gcaaggtgaa
    17221 tcaaccacgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccatgccga
    17281 cgcagaaccg ctggcggaag ctaccggcct gaagctgggt ctggcttacg gtggtgatgg
    17341 ctacgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg gcactacggg
    17401 gcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc aggtggtggt
    17461 actggacgaa gccgatcgca tgtacgatct gggctttatt aaagatatcc gctggctgtt
    17521 ccgccgtatg ccgcctgcaa accagcgcct caacatgctg ttctccgcca cgctttcgta
    17581 ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc
    17641 ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa
    17701 aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc
    17761 caataccaaa taccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt
    17821 cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac
    17881 ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc
    17941 ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg
    18001 tattggtcgt acaggtcgcg caggcgcaag cggtcactct atcagcctgg cgtgtgaaga
    18061 gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc cggtaagcaa
    18121 atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccacg
    18181 tacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat
    18241 aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat gaatcccgat
    18301 gagcatactg tagtaagtga ttcaggtgaa agaatgcagc caacacagag acagattgaa
    18361 ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata
    18421 gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaataa
    18481 aacgcaaagt gcgtctggct gctggcctga atagcgaaaa tgccctgtct aatgaagcaa
    18541 tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat atccctccct
    18601 cgcaaattcg cgttgtcgct acggcgacat tacgtcttgc cgtcaatgcg ggtgatttta
    18661 ttgccaaagc gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt gaagaggaag
    18721 cacgtctgat ttatcagggc gttgctcaca caactggcgg tgccgatcag cgcctggtgg
    18781 tggatatagg cggtgccagt actgaactgg taaccggcac gggtgcacaa accacctcgt
    18841 tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc
    18901 tggggcagga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg
    18961 ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg tgcttccggc accgtgcagg
    19021 cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc
    19081 agcaattgaa acagcgagcc attcattgcg gtcggctgga ggaactggag attgacgggc
    19141 tgacgctgga acgtgcgtta gtgttcccga gtggtctggc gatcctgatc gccattttta
    19201 ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct
    19261 acggcatgtt gcatctggcc gtcgagcagg atattcgcag ccgtacgctg cgtaatattc
    19321 agcgccgctt tatgatcgat attgatcagg cgcagcgcgt agccaaagtt gcggctaact
    19381 tcttcgatca ggtggaaaag gaatggcatc ttgaagcaat aagccgcgat ttgctcatca
    19441 gcgcctgtca gcttcatgaa atcggcctga gcgttgactt taaacaagcg ccgcaacacg
    19501 ctgcttatct ggtgcgtaat ctggatcttc ccggttttac ccccgcacag aaaaaattgc
    19561 tggcgacgct actgctcaac cagactaatc cggtcgatct ctcatcgctg catcagcaaa
    19621 atgccgtacc gccgcgcgtc gcagaacaac tctgccgttt actacgcctg gctatcattt
    19681 ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac
    19741 tgttgacctt gacgcttccg caaggttggc taacccaaca tccgctgggt aaagagatta
    19801 ttgctcagga aagccagtgg cagagctatg tacactggcc gctggaagtg cattaatgag
    19861 taagtgccgg atgcgatgct gacgcatctt ttccggcctt gattatttcc ctcgttttgc
    19921 cgccatcatc gctttcagat tcgccagatg gctttgccct ttctgcatcc gttcttcggc
    19981 gctgaccact ttgcgctcct gttcccaaat cagatcatcc tgcggcagct ccagcaaaaa
    20041 gcggctcggc tccgggcgca ccagctcgcc gtactgacgg cgttctttac acagcgtaaa
    20101 ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcgat
    20161 attatcttca tcgatgctgc tctggtgcgg caaaaaccct tcttccatac cgaccatgta
    20221 gacataagga aactccagcc cttttgacgc gtgcagagtc atcagttgca cctgatccag
    20281 ctcttcttca ctctcaccac gctccatcat gtcgcgcaaa gtaaagcgcg tcaccacctg
    20341 ggtgagcgtc atcggctcat ccagttcgct gccttccagc atctccgtca tccagctaaa
    20401 cagttggttg acgttcttca tgcgcatttc ggcggctttc gggctgggcg atgtttcgta
    20461 cagccaggat tcataatcca tgccgtggat cagatcgcgc accgcggcaa tcggctcccg
    20521 ctccgccaga cgctggattt ctgccaacca gtgagtaaag cgggtcaatg cttcataacc
    20581 acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt
    20641 gcgcgtcatc gcccactcac ccagcttttt cagcgtcgcc gggccaatct ctctcttcgg
    20701 cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca cgcgcagata
    20761 agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccaccag aaattttgta
    20821 cgggatgcgg ttttgcatca ggaatttttc aaacactcgc gactgatggt taccgcgata
    20881 aagaatagcg taatctttgt actgcgtttt attgacgaag tgatgcgcaa tcagttcacc
    20941 agtgacgcgc tcggcttcat gttcttcgtt attcgcgctt aaaactttaa gctctgtgcc
    21001 gtaacccagt tcggagaaca gacgcttctc aaagacgtgc gggttattgg cgatcaggat
    21061 gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt
    21121 cagcgccgga aaatcctgac tcagcagcac caggttttgt ggacgtgcgc cgcgccagga
    21181 gtagatcgac tggtcgtcat cgcccaccac ggtaaagcgc gcgcggctgc ccaccagcag
    21241 tttcaccagt tcatactggc tggtgttggt gtcctgatac tcatccacca gcagatagcg
    21301 aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcagcagca acgtcggcaa
    21361 taaaatcaga tcatcgaagt cgagaacatt gcacgctttc aggtgtgcat catacagccc
    21421 ataacaatgg gcaaaaatac ggtcccgctc gccaatcgca ctggctgccg cctgggacgg
    21481 tgttttgaga tcgttcttcc agttagagat ggtcgaaatc aactgttgca ggagaacttt
    21541 gtcatcttca atcagccctt cggtcaactc tttgagcaac gccagctgat cggtatcgtc
    21601 aaacagcgaa aagttcgcct tcatccccag cgccgcatac tcacgcttga tgatatccag
    21661 ccccagcgtg tggaaggttg agatcatcag cccacgcgcc tctttgcgac ccagcgtctg
    21721 tccgacacgc tctttcatct cgcgcgccgc cttattggta aaggtcaccg ccgcgatatg
    21781 tcgcgcctga taaccgcaac cgcggatcag atgggcgatt ttattggtga tcacacgagt
    21841 tttaccggaa cccgcgcccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg
    21901 ttgttggccg gggtttagac gcataggtgt attgctcaat cttcgaacgg ggggaggatt
    21961 gtagcagaat cgcatttgaa tgtgaccgcg tgtaagccgc taaccggttg gctgcggatg
    22021 tttaaatcag agagagaaga ccgaaaccgg agttcatcgt gaatccatta gacttagaaa
    22081 atatcgggtc gtaacgttgt gttattactt tttcagagtt tactaaggag aggtgtactc
    22141 gtggcgatta atttcagtcc taaggttggt gaaatactgg aatgcaactt tggtaactat
    22201 cctgttagcc agaatggaca gttcagcacc acgtattacg atggccgcat tcctcctgaa
    22261 atgataaaaa acaggttagt tgtcgtgctc aacggaaaaa ttaatggcaa cgcatgtatc
    22321 gtcgttccat tatcaaccac tcgcgatcat gacaagttaa atcgtggaat gcatgttgaa
    22381 atagccagta atgttatcaa tggcctgcaa ttctttgatc agcagattcg ttgggccaaa
    22441 gccgacctgg tacaacaagt cagtcgaaac cgattaaata gagcaaggac ttacagaggg
    22501 tatcttaatc aatgtttgcc acatgagttg gttgcagaca tacagagggc ggtcatcaag
    22561 tcaatcaatg ccatatcgct cataaattga caataaaatc accatccccg ataatgactt
    22621 tgtagcccat agaggcccta agttttctcc ccttgaggga cctgcgaagc ccggattttc
    22681 cgggcttttg ccatatatgt accagcccct ccctgactga caattacgtt agcatctgcg
    22741 gatcatgcta aaatcgccgc ctgacaatta ttcactcaag gcacgatcat ggcaaaaaca
    22801 gcagcagcac tgcatatcct tgtaaaagaa gagaaactgg ctctggatct tctcgagcag
    22861 attaagaacg gtgccgattt cggcaagctg gcgaagaaac actccatttg cccatcaggc
    22921 aaacgcggcg gtgatttagg tgaattccgc cagggtcaga tggttccggc gttcgataaa
    22981 gttgtgttct cttgtccggt actggagccg accggcccgc tgcacaccca gttcggttac
    23041 cacatcatta aggtgctgta ccgcaactaa tagcaaggcc ttctccagga gaaggccttg
    23101 agtgttttct ccctctccct gtgggagagg gtcggggtga gggcatcagc gcgcacttaa
    23161 cccgcaacag caatacgttt catatctgtc atatagccgc gcagtttctt acctacctgc
    23221 tcaatcgcat ggctgcgaat cgcttcgttc acatcacgca gttgcgcgtt atctaccgcg
    23281 ccttccggaa tagctttacc caagtcgccc ggttgcagct ctgccataaa cggtttcagc
    23341 aacggcacac aagcgtaaga gaacagatag ttaccgtact cagcggtatc agagataacc
    23401 acgttcattt cgtacagacg cttacgggcg atggtgttgg caatcagcgg cagctcgtgc
    23461 agtgattcat aatatgcaga ctcttcaatg atgccggaat cgaccatggt ttcgaacgcc
    23521 agttcaacgc ccgctttcac catcgcgatc atcagtacgc ctttatcgaa gtactcctgc
    23581 tcgccgattt tgccttcata ctgcggagcg gtttcaaacg cggttttgcc ggtctcttca
    23641 cgccaggtca gcagtttctt atcatcgttg gcccagtccg ccatcatacc ggaagagaat
    23701 tcgccggaga tgatgtcgtc catatgtttc tggaacagcg gtgccatgat ctctttcagc
    23761 tgttcagaaa gcgcataagc acgcagtttc gccgggttag agagacggtc catcatcagg
    23821 gtgatgccgc cctgcttcag cgcttcggtg atggtttccc aaccgaactg aattagtttt
    23881 tctgcgtatg ccggatcggt gccctcttcc accagcttgt cgaagcacag cagcgagcca
    23941 gcctgcaaca taccgcacag gatggtttgc tcacccatca ggtcagattt cacttccgca
    24001 acgaaggacg attccagcac gcccgcacgg tgaccaccgg ttgctgccgc ccatgcttta
    24061 gcgatcgcca taccttcgcc tttcggatcg ttttccgggt gaacggcaat cagcgtcggt
    24121 acgccgaatc cacgtttgta ctcttcacgc acttcggtgc cagggcattt cggcgcaacc
    24181 atgactacgg tgatatcttt acggatctgc tcgcccactt cgacgatgtt gaaaccgtgc
    24241 gagtaaccca gcgccgcgcc gtctttcatc agtggctgta cggtgcgcac tacatcagag
    24301 tgctgcttgt ccggcgtcag gttaaccacc agatccgcct gcgggatcag ttcttcgtaa
    24361 gtaccaactt taaaaccgtt ttcggtcgct ttacgccagg acgcgcgctt ctcggcaatc
    24421 gcttctttac gcagagcgta ggagatatcg agaccagaat cacgcatgtt caggccctgg
    24481 ttcagcccct gtgcgccaca gccgacgata accacttttt taccctgaag gtagctcgcg
    24541 ccgtcggcga attcgtcgcg ccccataaag cgacatttgc ccagctgtgc cagctgctgg
    24601 cgcaggttca gtgtattgaa gtagttagcc atggtgattc ctcgtgatgt tgtgcttctt
    24661 attgttcggt tcgcgtttgc gagattgaat tcactatatg acaggaaatt tattgcggaa
    24721 attgatatat tcacaacgtc acattgcaat ttctgcaacg tcaacattga gggctgcgcc
    24781 attggattta cgcgatctga aaaccttcct gcatctggcg gaaagccgcc attttggccg
    24841 cagcgcgcgg gcgatgcacg ttagcccgtc cacgctctca cggcagattc agcgcctgga
    24901 agacgatctc ggtcaggcat tatttgtgcg cgataaccgg acggtgacgc tgaccgaagc
    24961 gggcgaagag ttacgcgttt tcgcccagca aacattgttg cagtatcagc aactgcgcca
    25021 caccatcgat cagcaagggc cgtcgctctc tggcgagtta catattttct gctcggtgac
    25081 cgccgcttac agccatctac cgccgattct cgaccgcttc cgcgcggaac acccgtcggt
    25141 ggagattaaa cttactactg gtgatgcggc agatgcgatg gaaaaggtgg tcactggtga
    25201 agcggatctg gcgattgcgg gtaaaccgga aaccttgccc ggcgcagtgg cgttttcgat
    25261 gctggagaat ctggcagtag tgctgattgc ccccgcgctg ccctgcccgg tgcgtaatca
    25321 ggtgtcggta gagaagccgg actggtcaac ggtgccgttt atcatggccg atcaggggcc
    25381 ggtacgccgc cgcattgaac tgtggtttcg gcgtaacaaa atcagtaacc cgatgattta
    25441 cgccacggtt ggcgggcatg aagcgatggt atcgatggtg gcgctcggct gtggcgtggc
    25501 gctgttgccg gaagtggtgc tggaaaacag cccggaaccg gtgcgtaacc gcgtgatgat
    25561 actagagcga agcgatgaga aaacgccgtt tgagctgggc gtgtgtgcgc agaaaaagcg
    25621 gctgcatgag ccgctgattg aagcgttctg gaagatttta ccgaaccact aacccgccaa
    25681 aaagaacctg aacgccgggt tattggtttc gtcgtggcaa tcgtagccca gttcattcag
    25741 ccgggtttcg aaatccggtt catggtcgcc aagctcgaac gccgccagta cgcgcccgta
    25801 gtcggtaccg tgactgcgat aatggaacag agaaatattc cagtgcgtac ccagcgtgtt
    25861 gaggaagcgc agcagcgcac ccggcgattc cggaaattcg aagctgtaga gacgttcctg
    25921 caacggatgc gatggacgcc cgccaaccat atagcgcaca tgtagcttcg ccatttcatc
    25981 gtcggagaga tcaaccacgc tgtagccgcc gtcgttgagc atctgcaaaa tttctttgcg
    26041 ctcttcgagg ccgcggctta agcgcacgcc gacaaagatg caggcgtttt tggcatcggc
    26101 aaagcggtag ttgaattcgg tgaccgaacg tccgccaagc aactggcaga atttgaggaa
    26161 gctacctttc tcttccggaa tggttaccgc cagcaacgct tcacgctgtt cacccagctc
    26221 acaacgttcc gagacgtagc gcaggccgtg aaagttcacg ttagcaccgg aaagaatatg
    26281 cgccagccgt tcgccgcgaa tgttgtgctg ggcgatatat tttttcattc ccgccagcgc
    26341 cagcgcgcca gagggttccg ccaccgcgcg cacatcttcg aataaatcct tcatcgccgc
    26401 acagatcgca tcactatcga cggtgatgat gtcgtcgaga tactcctggc ataaacggaa
    26461 ggtttcgtca ccgatgcgtt ttaccgctac gccttcagca aatagcccta cgcgcggcag
    26521 atcaaccgga tgacccgcat ccagcgctgc tttcaggcag gcggagtctt ccgcttctac
    26581 ggcgatcact ttgatttgtg gcatcagttg tttgatcagc accgcaacgc ccgccgccag
    26641 accgccgccg ccgactggca caaatacgcg gtcgagatgg gcgtcctgct ggagcagttc
    26701 cagcgccagc gtgccttgcc cggcaatcac catcggatgg tcgaacggcg gcacccaggt
    26761 gaacccctgc tgctgtgaca gttcgatcgc tttagctttc gcttcgtcaa agttcgcgcc
    26821 atgaagcagc acttcgccgc cgaatccacg taccgcatcg actttgatgt cggcggtggc
    26881 ggttggcata acgatcaggg ccttcacgcc taaccgcgca gaagaaaacg cgacgccctg
    26941 cgcgtggtta cccgcagaag cagtgatcac gccgtgcgct ttttgttctt ccgtcaggcc
    27001 cgccatcatg gcgtatgcgc cgcgcagctt aaagctgtgc actggctggc gatcttcgcg
    27061 cttcaccaga atgacgttat caagacgcga cgacagtttt tccatttttt gtagcggcgt
    27121 aacctgcgcc gcctcgtaaa ccggcgcgcg cagcactgct cttaaatatt cggcaccttc
    27181 cggagcaccg gacaggggtt gcgagtcagc cattattacc cccccagttt cgatttatcg
    27241 cgcaccgcac ctttgtcggc gctggttgcc aggctggcgt aggcgcgcag ggcaaaggaa
    27301 acctgacgtt cacggttttt cggcgtccag gctttgtcac cccgagcttc ctgcgcttca
    27361 cgacgcgccg ccagttcggc atcgcttacc tgtaactgaa tgccacggtt cgggatgtcg
    27421 atagcgatca ggtcaccatc ttcaatcaga ccaatgctgc cgccgcttgc cgcttccggt
    27481 gagacgtggc cgatggaaag accagaggtg ccgccagaga aacgaccgtc ggtgatcagc
    27541 gcacaggctt taccgagacc cattgatttc aggaagctgg ttgggtagag catttcctgc
    27601 atgccagggc cgcctttcgg gccttcatag cggatcacca ccacatcgcc cgcgacaact
    27661 ttgccaccga ggatcgcttc taccgcgtcg tcctggcttt cgtacacttt cgccgggccg
    27721 gtgaatttga ggatgctgtc atcgacgccc gcggttttaa cgatgcagcc gttttctgcg
    27781 aaattaccgt agagcaccgc caggccgccg tctttgctgt aggcgtgttc cagcgagcgg
    27841 atacagccat tggcgcgatc gtcatcgaga gaatcccaac ggcaatcctg cgagaatgcc
    27901 tgtgtggtac gaatgcctgc aggacctgcg cggaacatat tttttaccgc atcatcctgg
    27961 gtcagcataa cgtcgtattg ttccagcgtt tgcggcaacg tcaggccaag tacgtttttc
    28021 acatcacggt tcagtaaccc cgcgcgatcc agttcgccga gaataccgat aacaccacca
    28081 gcacggtgaa catcttccat atggtatttc tgggtgctcg gcgcaacttt acacagctgt
    28141 ggaaccttgc gggaaagctt atcgatatca ctcatggtga agtcgatttc cgcttcctgc
    28201 gccgccgcca gcaggtgaag tacggtgtta gtcgatccac ccatcgcgat atccagcgtc
    28261 atggcgtttt caaacgccgc cttactggcg atattacgcg gcagtgcact ttcgtcgttt
    28321 tgctcgtaat aacgtttggt caattcaaca atgcgtttac cagcattaag gaacagctgc
    28381 ttacggtcgg cgtgggttgc cagcagcgag ccgttgcccg gctgcgacag gcccagcgct
    28441 tcggtcaggc agttcattga gttagcggta aacatcccgg agcaggaacc gcaggttggg
    28501 caggcggaac gttcaacctg atcgctctgg gagtcagaaa ctttcgggtc tgcgccctgg
    28561 atcatcgcat caaccagatc gagtttgatt atctgatcgg acagtttggt tttcccggcc
    28621 tccatcgggc cgccggaaac aaagatcacc ggaatattca ggcgcaggga agccatcagc
    28681 atccccgggg tgattttgtc gcagttagag atgcagacca tggcgtcggc gcagtgggcg
    28741 ttgaccatat actcaacgga atcagcgatc agttcgcgag atggcagtga ataaagcatc
    28801 cccccgtggc ccatggcaat cccatcatcc accgcaatgg tgttgaactc tttggcaacg
    28861 ccgccagccg cttcaatttg ttcggcgacc agtttaccga gatcgcgcag atggacgtga
    28921 cccggtacaa attgggtgaa cgagttcaca accgcgataa tcggcttacc gaaatcggcg
    28981 tcggtcattc cggtggcgcg ccacagcgca cgagcacccg ccatattacg accatgagtg
    29041 gtggtggcgg aacggtactt aggcatactt tatttactcc cagtgtctgt ctcgtaaatg
    29101 ggacggtgcg tgccgtccca ttttttgtat ttattgatta acttgatcta accagcccca
    29161 tttatcttcg gtttcgccag tgaagaggcc gaagaaggct tgctgaatgc gtttggtaac
    29221 cgggccacaa cggccttcgc caacctgaat accgtctacg ctgcgcactg gcgtgatttc
    29281 tgccgccgta ccggacataa acacttcatc cgccaggtac agggattcgc gcgacagcac
    29341 ctgctcacgt acttcaattc ccagctcttt cgccagtttg atgatggcat cacgggtaat
    29401 acccggcagc gcggaggagg tgaacggtgg ggtgaacagc acaccatctt tcacttcaaa
    29461 caggttttcg cctgcgcctt cagagatata accgttcaca tccagcgcga taccttcctg
    29521 ataaccgtgg cggcgcgctt cgctacccac cagcagggaa gagaggtagt taccaccggc
    29581 ttttgccgcc gtcgggatgg tgtttggtgc tgcgcggttc caggaggaaa ccatcgcatc
    29641 gatcccctgc tccagcgctt ctgcgcccag atacgctccc cacgggaaag cagcgataat
    29701 cacgtcggtt gagtatcccg ctggcgggtt tactcccatg ccaacatcac cgacgaagat
    29761 cagcggacgg atataggcgc tggtgagatt atttttgcgg atcacgtcac gacaagcttc
    29821 catcagctca tcaatgctct gcgaaaccgg gaagcgatag attttggcgg agtcatgcag
    29881 acgctgcata tgctcacgat ggcggaatac aaccggtcct ttgtgcgagt cgtagcaacg
    29941 gatgccttca aaaaccgaag tgccatagtg cagcgcgtgc gacatcacat gcaccttcgc
    30001 gtcttcccag cgaaccatct ccccattgaa ccaaatgtaa tcagctttct tcgtggtcat
    30061 ttttatattc cttttgcgct caggcgcgga tttgttgtga tgtggttgtg ctctggcaga
    30121 tggcaacgtg tgcgacgtcc accagtttat ttaactgact aaacagtaag tcgaccgacc
    30181 gtgggctggc aacggtcaat tcgatattta tattttgtgc atcgctggcg gcggccatat
    30241 tcattgagca gacgtggaaa ccacgatgac gcaccacgcg taaaacacgt tctaaggttt
    30301 ccggattgaa gcgagccgat acattgacct gatgttgcat catgataatt tctccaacat
    30361 ttctgaatta ctggcaccag gcggcaccag cggccagacg ttctcaagtt cgtcgattga
    30421 gacatgaagc aggtatggcc catcactgtt cagcatggtg tcgagtgccg cttcaacctg
    30481 gtctttacgg gtgatgtgtt ggccagggat gccgaaggcg ctggctaaca tgaggaaatc
    30541 ggggttatca gtaagggtgg tttcgctgta tcgttcctga aaaaacagtt gctgccattg
    30601 tcgaaccatc cctaaccgtt ggttatcgag taagacgatt ttcaacggta actgcttgcg
    30661 ttttacggtg cccagctctt gcacattcat catgaaagag ccgtcaccgg agatacagac
    30721 gacagtatcg ttcggtcgtg cgacttgtgc gccaaccgcc gctggtaaac cgaaacccat
    30781 ggtgcctaag ccgctggagg taatgaaatt ttccgggcga gtgtgtgcga tgtgctgcgc
    30841 ggcccacatc tggtgctgcc ccacatctgt ggtcacgacg caatccgcag gtttacgatc
    30901 cgacagttgt tttaacaaca acggcgcgta gatagcgtca ccgggatggt cgtaacgcca
    30961 ggtatgttca tcacgcagct gcgcgcagtg ttgctgccag tcattgatat ttaacggctg
    31021 ctgtaatgct ggtaacagag catttaaatc accttgtaat gccacatgtg cctgacgcag
    31081 cttgttcatt tctgccgggt cgatatccat atggataaca ctggcgtgtg gtgcgaaggt
    31141 gttcagtttg ccggtcaccc ggtcatcaaa acgtgcaccc acggcgatca gcaggtcaca
    31201 ctcctgcacc gcgaagttcg ccgctttggt gccatgcatt cccagcatgc ccaggtagta
    31261 cggataatct gcttcaactg cgcccagccc tttcagcgtg caggtggcag gcatttttgt
    31321 ggtagcgaga aattctcgta aagcaggaac tgcctgcgcc atacccacgc caccaccaac
    31381 gtacagcatc ggtttttgcg cttttgccag catctggcgc gcttgctcaa cttcggcatg
    31441 ggggaaagtc acttcgtttt caacggtggt gaaccacggt tccagatcgc cgctggctaa
    31501 ctggatatct tttgggatat cgaccagaac cggaccagga cgacctgagc tggcaacgtc
    31561 gaatgcttca gccataatgc gcggcaactc ttccagcgac tgcaccagaa agctgtgctt
    31621 ggtacaggct aacgacaatc ccaggacatc cacttcctga aatgcgtccg tgccgataaa
    31681 cggtgcggac acttgaccgg tgatggcaac aacagggata gaatctaaca gtgcgtccgc
    31741 aagcccggtt atcaggttgg ttgcgcccgg accagacgtg gcgatacata cgccagtttt
    31801 gccggtagca cgggcataac cgatagccgc cattgccgca ccctgctcat gtcggcacag
    31861 caagtgctcc acgccgccgt catacaatgc atcgtaaacc ggcataattg cgccacccgg
    31921 ataaccgaaa acggtgttca caccctgtgc ccgcaacgca tgtaccaccc actgtgcgcc
    31981 attcatagtt agttccccgt cctgaatctt gagaaacaga attttgtgct gttattcatt
    32041 gtctgctcct cggttatgtt tttaaggtca aaaaaaaccc ccggaccttt cggtgcgggg
    32101 gtcttagttc gttaaggctt gatctctaag cctttcctcg tccaagtgca gccccgcacg
    32161 gtgggataat aatcaccacc acgctaatca cgaccaggct aatcactcgt agaagggctg
    32221 tcattttgtc ttttcttgca tcttgttcga aggaatgcct aaagagttac catagatttt
    32281 gcgaatagca caagatattt tttggccgat ttttatgatg cgtttaataa tttcaataga
    32341 acttgttgtt ttttaaaaag aaaatagtga gtgaaaaaaa ttcaatttca gtatgaatgt
    32401 tgttcccttc ctcgtagttc atcctttgtc cgctgcattt ttgcgaacat cattccaccg
    32461 ccagaacaac tcaacttcac ttgcacaaat aaacaggaat cagccactaa aattcacgaa
    32521 ttagcgggct acagcagcga tatcttatgc acataatagt gactcaagga gggcttatgt
    32581 cactgtcaat tgttcatacc cgcgcagccc tgggagtaaa tgcgccccca atcactgttg
    32641 aggtacatat cagtaaaggt ctacccggct taacgatggt gggcttacca gaaacaactg
    32701 taaaagaagc tcgcgatcgc gtgcgcagcg ccattatcaa tagcggatat gaatatccgg
    32761 cgaaaaaaat caccatcaac ctggctccag ccgatctgcc aaaagaaggg ggacgatatg
    32821 atttacctat cgccattgcg ttgctggcgg cctcagaaca gcttacagcc aataagttag
    32881 atgaatatga attagtcgga gaactggcgc ttacaggcgc tctgcgtggc gttcccggcg
    32941 caatctccag tgcaactgaa gctattaagt cgggcagaaa aattatcgtc gcgaaagata
    33001 acgaagatga agtggggcta attaacggtg aaggatgcct gatagccgat catctgcagg
    33061 ctgtctgtgc gtttctggaa ggtaagcacg ctctcgaacg cccgaaacca actgatgcag
    33121 tatcccgggc gctacaacat gatctcagtg atgttgtcgg tcaggagcaa ggaaagcgag
    33181 gactggaaat taccgccgct ggcgggcaca accttttact gattgggccg ccgggaacag
    33241 gtaaaacaat gctcgccagc cgtattaatg gcctgttgcc agatttaagc aatgaagagg
    33301 cactggagag cgctgcgata ttaagtctgg taaatgctga atcagtacaa aaacaatggc
    33361 ggcagcgccc gttccgctca ccgcatcaca gtgcgtcgtt aactgcgatg gtgggcggtg
    33421 gtgcaattcc agggccaggt gaaatttcgt tggcgcataa cggcgtgctt tttcttgatg
    33481 agttacctga atttgaaagg cgtacactgg atgccttgcg agagccgatt gaatccgggc
    33541 agatccatct ttcacgcacg cgagcaaaaa taacctatcc agcccgtttc cagcttgtcg
    33601 cggcgatgaa tcccagccct accggacatt atcagggaaa ccataaccgc tgcacaccag
    33661 agcaaacgct gcgttatctc aaccggctct cggggccctt tctcgaccgc ttcgatctct
    33721 cactggagat cccattacca ccccccggca ttttgagtaa aacggtagtg ccgggagaaa
    33781 gcagcgccac cgttaaacaa cgcgtaatgg ccgccagaga gcgccaattt aagcggcaga
    33841 ataaactgaa tgcctggctg gatagtccgg aaatacgcca attctgcaaa cttgagaacg
    33901 aagatgcgat gtggttggaa ggaacactga tccatctggg gttatcgatt cgtgcctggc
    33961 agcggttatt gaaagttgca cgaaccattg ctgatattga tcagtctgac attatcacac
    34021 gtcagcattt gcaggaggca gttagctatc gtgcgattga ccgtttgctc atccatctgc
    34081 aaaaactact gacataaaaa aagggcattt cgcccttttt attaatcatc agaatcggtg
    34141 tagtcttcag caccttcaac ctgcggttta ccgccggaaa gggtgtgaaa acgttttgga
    34201 cgcttaatac gcgtcatata cttggaccac acgcgttctg cttctgtcac tggctcacgt
    34261 tcgccacggc atactgctac gaagagtttc tcttcctcgg taaccggctc gcgtttgcca
    34321 agatccaact cattgaaggc ataaccatga cgctcaagca gttgtgcctc tttgatggtg
    34381 aaatcaccat gacgagagaa tccacgtgga taatgtttat tgtcgaaata tcgattagtc
    34441 gtcgtaaagc tttccgccat cctgcacgct cctaattctt tgaccgagct agttatggcg
    34501 cggagtatta gttacgcttg acagagtgta aaacaaaaca tttaaatcat aacgacaaat
    34561 aattttgcgg agagcactgt ggatacggaa ttgttaaaaa ctttcctgga agttagccga
    34621 acgcgtcact ttggtcgagc ggctgaatcg ctctatctga cccagtcagc agtgagcttt
    34681 cgaatcagac aactggaaaa tcaactgggt gtgaacattt tcacccgcca cagaaacaat
    34741 atccgtttaa ccgctgccgg tgaaaaacta ctgccttatg cagaaacgct catgagcacc
    34801 tggcaggccg cccgtaagga ggtggcgcat acctcacgac ataacgagtt ttctatcggt
    34861 gccagcgcct cgttgtggga atgtatgctt aatcagtggc tgggacgctt gtatcaaaat
    34921 caggatgccc atacaggctt acagttcgaa gcgcgaattg cccaacggca gtctctggta
    34981 aaacagctgc atgaacgcca gcttgatctt cttattacca ctgaagcgcc caaaatggac
    35041 gaacttagta gtcagttgct gggatatttc actttagcgc tttataccag tgccccttca
    35101 aaactaaagg gagatcttaa ttatctgcga cttgagtggg ggccagattt tcaacagcat
    35161 gaggcaggtt tgatcggtgc tgacgaagtg cccattctga caaccagttc tgctgaactg
    35221 gcacagcaac agattgcgat gcttaatggt tgcacctggc tacccgtcag ctgggcgcgt
    35281 aaaaaaggcg gcctgcatac cgttgtcgat agcacaacac tttcacggcc gctttatgcc
    35341 atatggctgc aaaatagcga taaaaatgcg ttgattcgcg atcttttgaa aattaacgtg
    35401 ctggatgaag tgtattaata tgaatggctg gcaaggatgc cggtagaagg atttacttcg
    35461 gagagggtta tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggcagg
    35521 ggcggagaga ctcgaactcc caacacccgg ttttggagac cggtgctcta ccaattgaac
    35581 tacgccccta attag
//
LOCUS       DAZGSP010000048        34303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZGSP010000048 DAZGSP010000000
VERSION     DAZGSP010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.048"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(119..361)
                     /gene="yiiF"
                     /locus_tag="ACHK74_002545"
     CDS             complement(119..361)
                     /gene="yiiF"
                     /locus_tag="ACHK74_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFP0221344.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(580..798)
                     /gene="yiiE"
                     /locus_tag="ACHK74_002546"
     CDS             complement(580..798)
                     /gene="yiiE"
                     /locus_tag="ACHK74_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFP0221345.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(1384..1674)
                     /gene="nadS"
                     /locus_tag="ACHK74_002547"
     CDS             complement(1384..1674)
                     /gene="nadS"
                     /locus_tag="ACHK74_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFP0221346.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(1675..1986)
                     /locus_tag="ACHK74_002548"
     CDS             complement(1675..1986)
                     /locus_tag="ACHK74_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0221347.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            2215..3123
                     /locus_tag="ACHK74_002549"
     CDS             2215..3123
                     /locus_tag="ACHK74_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0221348.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(3187..4128)
                     /gene="fabY"
                     /locus_tag="ACHK74_002550"
                     /gene_synonym="yiiD"
     CDS             complement(3187..4128)
                     /gene="fabY"
                     /locus_tag="ACHK74_002550"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFP0221349.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(4173..4610)
                     /gene="dtd"
                     /locus_tag="ACHK74_002551"
     CDS             complement(4173..4610)
                     /gene="dtd"
                     /locus_tag="ACHK74_002551"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFP0221350.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(4607..5479)
                     /gene="yihY"
                     /locus_tag="ACHK74_002552"
     CDS             complement(4607..5479)
                     /gene="yihY"
                     /locus_tag="ACHK74_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFP0221351.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(5473..6072)
                     /gene="yihX"
                     /locus_tag="ACHK74_002553"
     CDS             complement(5473..6072)
                     /gene="yihX"
                     /locus_tag="ACHK74_002553"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFP0221352.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(6213..7100)
                     /locus_tag="ACHK74_002554"
     CDS             complement(6213..7100)
                     /locus_tag="ACHK74_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFP0221353.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(7090..8439)
                     /locus_tag="ACHK74_002555"
     CDS             complement(7090..8439)
                     /locus_tag="ACHK74_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0221354.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLILMALRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(8538..9971)
                     /locus_tag="ACHK74_002556"
     CDS             complement(8538..9971)
                     /locus_tag="ACHK74_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0221355.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASSTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(10041..11051)
                     /locus_tag="ACHK74_002557"
     CDS             complement(10041..11051)
                     /locus_tag="ACHK74_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFP0221356.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(11084..11971)
                     /locus_tag="ACHK74_002558"
     CDS             complement(11084..11971)
                     /locus_tag="ACHK74_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFP0221357.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(11996..12874)
                     /gene="yihU"
                     /locus_tag="ACHK74_002559"
     CDS             complement(11996..12874)
                     /gene="yihU"
                     /locus_tag="ACHK74_002559"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFP0221358.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPCKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            13047..13943
                     /locus_tag="ACHK74_002560"
                     /pseudo
     CDS             13047..13943
                     /locus_tag="ACHK74_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            13983..14771
                     /locus_tag="ACHK74_002561"
     CDS             13983..14771
                     /locus_tag="ACHK74_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP0221359.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(14882..16147)
                     /gene="yihN"
                     /locus_tag="ACHK74_002562"
     CDS             complement(14882..16147)
                     /gene="yihN"
                     /locus_tag="ACHK74_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0221360.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(16249..17229)
                     /gene="yihM"
                     /locus_tag="ACHK74_002563"
     CDS             complement(16249..17229)
                     /gene="yihM"
                     /locus_tag="ACHK74_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFP0221361.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(17237..17947)
                     /gene="yihL"
                     /locus_tag="ACHK74_002564"
     CDS             complement(17237..17947)
                     /gene="yihL"
                     /locus_tag="ACHK74_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP0221362.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(18164..19987)
                     /gene="typA"
                     /locus_tag="ACHK74_002565"
     CDS             complement(18164..19987)
                     /gene="typA"
                     /locus_tag="ACHK74_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFP0221363.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            20360..21769
                     /gene="glnA"
                     /locus_tag="ACHK74_002566"
     CDS             20360..21769
                     /gene="glnA"
                     /locus_tag="ACHK74_002566"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFP0221364.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            22088..23137
                     /gene="glnL"
                     /locus_tag="ACHK74_002567"
     CDS             22088..23137
                     /gene="glnL"
                     /locus_tag="ACHK74_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFP0221365.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            23149..24558
                     /gene="glnG"
                     /locus_tag="ACHK74_002568"
     CDS             23149..24558
                     /gene="glnG"
                     /locus_tag="ACHK74_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFP0221366.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            24670..24780
                     /gene="yshB"
                     /locus_tag="ACHK74_002569"
     CDS             24670..24780
                     /gene="yshB"
                     /locus_tag="ACHK74_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFP0221367.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(24970..26343)
                     /gene="hemN"
                     /locus_tag="ACHK74_002570"
     CDS             complement(24970..26343)
                     /gene="hemN"
                     /locus_tag="ACHK74_002570"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFP0221368.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(26532..27041)
                     /gene="yihI"
                     /locus_tag="ACHK74_002571"
     CDS             complement(26532..27041)
                     /gene="yihI"
                     /locus_tag="ACHK74_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFP0221369.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQEELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(27160..27326)
                     /locus_tag="ACHK74_002572"
                     /pseudo
     CDS             complement(27160..27326)
                     /locus_tag="ACHK74_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27623..28255
                     /gene="yihA"
                     /locus_tag="ACHK74_002573"
     CDS             27623..28255
                     /gene="yihA"
                     /locus_tag="ACHK74_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFP0221370.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(28637..31423)
                     /gene="polA"
                     /locus_tag="ACHK74_002574"
     CDS             complement(28637..31423)
                     /gene="polA"
                     /locus_tag="ACHK74_002574"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFP0221371.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWEQAH"
     gene            31787..32719
                     /gene="yihG"
                     /locus_tag="ACHK74_002575"
     CDS             31787..32719
                     /gene="yihG"
                     /locus_tag="ACHK74_002575"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFP0221372.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWSLDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(32760..34190)
                     /gene="yihF"
                     /locus_tag="ACHK74_002576"
     CDS             complement(32760..34190)
                     /gene="yihF"
                     /locus_tag="ACHK74_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFP0221373.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     IMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         8682 a         8773 c         8120 g         8728 t
ORIGIN      
        1 aataaacttc aatatattga atttacatga ctttgtaggc ctgataaggc gctcacgccg
       61 catccggcat gaacaacgcg cacgttgtca gcaatctgat tgacatcatt ttttcccatt
      121 accattcctc gcgcagctta cgctgatatt cgacaccatc ttcctcacac ccttgccaga
      181 tgccaggaac actggttctt gctgtttgcg attgatttat caggtattga tctaccgctt
      241 cccttaatag gtccgcacga ggaagattac gctgcacctc gagatcatca agttgcttaa
      301 tcacctcatt cgataaatcg agtaaaattc tgcccatatc tatacctgcc agagagttca
      361 taaatatctc gccaatatca tttttgaatc tatggagaga aaagtaccct tgccgaatct
      421 ttaaagaaag ctcatttacg catcattttt tatttttgcg aggcgttttc cagatacgat
      481 gtaacagttg cagttattat aagttcaagt aaaacagcct tgtattaata ccagagtaag
      541 ggcatctgtt tttttatatt caagaatgaa aaattttttt cattccttat gctccttaca
      601 ctgatcatca acaccaacta cctccccctt ttgcctgcct ccctttgctt tacgcaccgg
      661 aaattgctgc cgctccagat attgctcaac cgcttcgcgt aaaattgcag atcgtgaaac
      721 cttacgccag ccccgaagtt tgttcagccg tttaattgcc tcttctgata aatgaagaaa
      781 aaccgtattc atcgccatta ccaccacctt acctatatta ctcattaatg tatgtgcgaa
      841 aattacgccg gaaaatcatt taaaaaaagc ggatttttgc atgttgtttt gtttttgcga
      901 ggcgttttcc agaaataata agagtgttgc agttgtttca tttattactc aaaaatggtt
      961 tagttatcgc ccaggatgaa ttatgggtgc tatgaatata ttatattcag agatatttat
     1021 ttgcataatc tgaatggtgt agccgaaaaa cgtcagcatc ctaacagcac caggatttaa
     1081 ggtgaaatta atctttcatc acagaccggt cagtactgcc accaaccggt ctttacttgc
     1141 tcatcgttat aattttgttg gtaacagttc gggaaacaag gaattccttc cggaaccccc
     1201 cggtatcatc cgatagcatt gacgtcaatc atcagcgcga cgatgagttt ttttaatcgc
     1261 atcatagggt ggcgacgttc agcacgatta cgggtcatag caaaactcct gggcaggcga
     1321 ccactatgcc gctttcacag gagttctgaa tgctagtgct ttctgtatgg cataaccatg
     1381 cctctaacgg ttaagtgctt gcagcacatg ttgcggatca ttggcaatcg ctcgcagcaa
     1441 agcctttgca ggcccggtcg gtgaacgtcg cccctgctcc cagttacgca acgtgtctac
     1501 gcttacagaa ataagatcgg caaatttaga ttgtgacaac cctgaagcgc ggcgtatttc
     1561 tttgattttc atagcatcaa cttcaaaagt ccgcgaaggc tgtcgcgttc cttcaataat
     1621 ttcattcatc tgggccatgc tgtgagtcaa ccgctcaaaa agtgccttat ccatttacca
     1681 cctcgtattt aacaaccgga gcattgcttt ttcctgggga gacagatcat ctttaatacc
     1741 cttacgataa ataagcagta atctgatctc ataatgttta acctgatgaa agtaaataac
     1801 cctgacacca gcacgtttcc cctttcctcc tgaaacccat cgaacctttc ttaacccacc
     1861 agtttcaggt attacctcac catagtcagg attcaaggcc aggaaaaact gaaaacgact
     1921 gaattcatcg tcggtcagca gtttttgaac atcttcagta aaaatttccg tttcgatgaa
     1981 gagcataatt atacgccatt ggcttagagt aacaatcctt tttgtacgtc attgacgtac
     2041 ccttgaaacc actagcttac tgatagtgct ccagcaaaac cacgtctctg ccgggtttgt
     2101 ctttagattt gcgaggcgct ttccagattt ttcacgcccg tgaatttccc tgtatccatc
     2161 cagcaaaaac gggtgtataa gactgtacat tcatacagca aaataagttc tactatggca
     2221 ctggaaaaag gaattgcaga gttagtcgaa aagtttatcg ctgcgggtcg cccctcatcg
     2281 cgtcaacaaa gtattgcgca gcgcagggag ggctatatcg ccagcgctgt ccttgcgggt
     2341 gagacagaaa cgcgggtaga tattcagact atcgaacttg aagggatgac cttacgaata
     2401 gtgtcgccgc tgaatgcccc gacattactc ccgacgataa tttattacta cggtggctgt
     2461 tttgttagcg gcgggttcgc aactcacgac aaccagctgc gccagttagc ctattacggg
     2521 caatgtcggg ttattgccgt ccagtatcgg ctggcaccgg aacatacttt ccccgccgcc
     2581 catgatgatg cgcaacgagg tgcagaaata gtctggcaac acgccgaacg actcggcgtt
     2641 gataaacaac gtataactct tgccggtgac agtgcgggcg gacatctggc gctggtaact
     2701 gctttgcgat taaagcgtgc tggcgagtgg caaccggcac aactcatctt aatttatcct
     2761 atgcttgatg cgactgcgca tttcgaaagc tacatacgca atggtcacga ttacatcatc
     2821 acacgcgaca ctttactgag cggctttgag atgtatctgc caggcattga acgcaggcat
     2881 cctgaagcca gccctctttg gcgcgatgat ttcaatggct tgccacctgt acatatcatt
     2941 accgctgaat acgacccact ttgcgatgaa ggtgaagcgt tgtatcaccg catgacaggg
     3001 caaggagtgc aatgcacctg ccaacgctac cttggcgtta tccacggatt ttttcagtta
     3061 ggtggtatca gcgaggccgc gcgatcggca ctacgggatg ttgcctggag agcgggaagg
     3121 taacttcgga cctgggccct gaaaaggttt tcagattgaa aaccttttca gtcaagtaaa
     3181 acgcccctac tcttcttcgt tcccgccctc ttcatacggg ccaaatggct tcgcgggcag
     3241 aacgatatac gtgccttcaa acactgcacc cggcgtctcg tcgccaaaga tttcaacttg
     3301 catctgcacc cgtgcttttc gtccgcgcgc cagacggtcg agatcgccgc ttaaggcacc
     3361 aaggtcggct accgcatgag gtttaccgct aatcggcttg ctgtagcgga tatgcgcatc
     3421 cgccagaata atcgttccgc cgagatggcg ttcgcgcagc atcagccaga taagccccca
     3481 accggtgagc gtcgccagcg agaataaact cccggcaaac agcgtatggt gcggattctg
     3541 attgccggtt tccggcatgg tggtgataaa tttttgcccg gtatattgtt gaatacgcac
     3601 gcccattttt tcactaagcg ggatatgttc gtaccacgcc tgttgcagct gcgcgcacca
     3661 gtcgccgcga tgcaaaatat catccagagt ggcgacgggc ttaatcatca aaaaatggcg
     3721 aatcggcgtg gtggttggcg tggtgatttc tccctgatta acaaacccca gcttggcgaa
     3781 aaactccacc gcgtcttcac gggcgctaca ggtcacgcgc ttaacgcctt cctgacgcgc
     3841 caccgactcc agggtcatcg ccatcagcgt tcctaaccct ttgtcctgca cgtccgggtg
     3901 aacggccata aagcgaatgg acgcttcatt gtcggcatta atatacagtc ggcctaccgc
     3961 caccagatta ccctgctcgt cgacgaccat ctggtgatgc gccatcgcat cccacgcgtc
     4021 gcgttccgaa ccttttggtt gatgcagggg cttacgcaac atttcccagc gaaactgata
     4081 gtaacgctct aattcttctt ctgtttgtgg aacccgaagg tgatacatag cgatactctc
     4141 tcttgtttcc cgtgacaacc ctggaagctg gctcatacct gcaaccagaa tgtcacgggg
     4201 ccatcattga ccagcgatac ctgcatatcg gcagcgaagc gtcctgtttg cgtgttcatc
     4261 tcttgctggc ggcagcgttc gacgaaatag tcatataacg cctctgcgcg atccggtgat
     4321 gcgcctttgg agaaacttgg gcgcatccct cgttcggtat ctgcggcgag ggtaaactgg
     4381 gaaaccacca gtacactgcc gcccgcctgt tgcacgttga gattcatctt gccttcggca
     4441 tcgctaaaga tgcggtagcc gagcacacgc tcgcacagac ggtttgcttt ctgttcgtcg
     4501 tcatcctttt cgacacccaa taacaccaaa agtcccgcgc caatttcgcc cgtcacttct
     4561 ccctccacgg tgacgctggc acgggttacg cgttgaatta atgcaatcat ggttcgtcgt
     4621 cttcttcttg ttcagctgct tgttttagtt tgcggtattc cccgagagtg acagtaattt
     4681 ccgcgccaag caagacgata caccatgtcc agtagaccca aacaaagaga atggggatca
     4741 ccgccagcac gccgtaaatg agctgatatg acgggaacat ggtgatataa agcgcgaaac
     4801 ctttctttcc tgcttcgaac aggagtgcag cgacaaacgc gccgacaatc gcgtcgcggt
     4861 taggtacgcg gatggtagga acaatgctgt acagcaacca gaaggagatc cacgacaaca
     4921 gcagcggaaa aatacgcagc acgttgtcga tgacagtatt gagatcgctc gcccagcgca
     4981 gggagagcaa ataggaactg atcgccagac tggcccctgc cagcagcggc cccagcgtta
     5041 aaatcatcca gtacacggca aacgagtaaa tttttggtcg cgctcgttta ctgcgccaga
     5101 tggtattcag cgcgctatcg atggagtaca tcaacaataa tgcggtgacg atcagcccgc
     5161 acgcgccaac ggcggtcatc ttgttggaat tggcaacaaa ttgttcgata taccgctgaa
     5221 taacatcgcc agtagcaggc agaaagttgg caaaaataaa gtgacgcaac tgaatgctga
     5281 cgtcggaaaa catgggaaaa gcggcgaaaa gcgcaaaaac aacggcaacc agcggcacta
     5341 atgagagcaa agacacatag gcaaggttac ctgccagggt tgtcatgttg tcctcatcaa
     5401 tacgttgcca gagtagtttt agccaggccc atagtggacg agtacggtgc ctggctttgt
     5461 cctgaatggt ttttagcata acaccttcgc gaaatagtcc gggatggtgg ttttatcttt
     5521 caccagaata ctggtaatgc ccagctgatt ggctccttct atattatcgg cgttatcgtc
     5581 gaaaaagacc gtatcgctgg gtgaaaaacc ttccgcctgc aaaacatgct ggtaaattcg
     5641 tgcttcaggt ttgcgcatcc ccagatcttg cgacagatag atatggtcag cagcatcacg
     5701 aatttccggg tattcttccg gccagaaggt ggtatgcagg cggttggtat tggaaagcac
     5761 caccacgcga tgcccctgct cacgcagttt atgcatgatg gcgatcactt ccgggcgcag
     5821 cgcaacaaac accgcctgcc agccgtgaga gaactgctcg tagcttagcg gtagagccat
     5881 ctcatgacac agcgcctctg cgaacgcttc gtcgctaatt tccccacgct catgctgatg
     5941 aaacgcctcc cccatatgaa aactcttctt aagcgatgcc agcggaatac gcgttaaatc
     6001 gctccaggct cccagcacac ggttaaagtc gatatcgaca atcacattac ctaaatcaaa
     6061 gatatagagc atttttgcct cctttcgcgc cacaagaaat taactgtagc gggaaaggat
     6121 gggtttggct atgtggcaat aagggtaatc ttgtgatgct gattgcgaaa aagcagagta
     6181 atttcactgg ctttttcgca atagatgaat ggttaagatt caggtgtaaa ttgccaacga
     6241 gtttcgatga ccagcggcct atctggtgtc accaattccg gcgttagatt tattgcgtta
     6301 ggtgcctgag tcagcgggtt aatacaggtg gcatcgggct gtttatcaaa taccaccaga
     6361 ctgtttgcag aagagatcat ggtcatcgcg agtttaccag gccacagtaa cttaaccttc
     6421 accccgtcat aaaaaccaaa acagtcatcc cagggaccgg cttgaggaga gatacgattt
     6481 ccagttggca gttcatcact acccgcttct tcttgccagt ccgcatcaaa aagcacctgc
     6541 aacgattctg tattttgcgg agtcagcttt ttcgcaaacc acggatgcca gcctgcggag
     6601 gccgggaatg tatcggcata tgaatggact tccagttgca aaaccagcgc atcgttctcc
     6661 agcaaaaaag tctgaatcac ttccccttgc cagggccacg gtgaagccag gggcatacgt
     6721 aaagtcaggg aatctgctgt tttgtcgatg atttcccacg ttgagtaaca agccatacca
     6781 tgcaacgcat gtggtggttt attggcgggt aaagaataac attgcccacc agcattcagc
     6841 gtcgcattcc ccagtcgccc tgcccacggc accattggga aacagccata ctgaaaagct
     6901 ctgcgctgcg gctgccattg gcgtaatact tcagaaccaa atgcctttaa cgaggttatc
     6961 ctggcaccat catgcggata aatctgcagg tgaatattgc ctttttccag cgtcactaac
     7021 tctcctacag gcccaggcca tggcgcgtca aacgcctgtt gtagcgccag gtaatgctcg
     7081 ttttttcgtt cagttttcat gagattgttc cctgatgtag cggtaagaag cggtttcttg
     7141 ttcatcggtc ggctcaatca gccagacgca aacaaaggaa agaactgaga cagcaaataa
     7201 aaacagagcg acataccacg gttttcctcc ccctagcgcc agcaaacttg aggcgataag
     7261 ggggcttagt ccgccaagaa tcgcggaaaa ttgataagca aaagaaaggc cggtatatcg
     7321 tactttggta ccgaacatgc gggtgaaaag tgttggctgc acggcaaaca tcatcgtcgg
     7381 acctaagttg tagccgagga tcatactgca ccagatgatg agcgtacttt tgctatccag
     7441 taataagaag aaaggaaacg cgaacaggac acaaaatcct gctcccgaca aatacagact
     7501 tttttgacct atacgatctg ataaccatcc aaccaatgga caactgaaaa taccgatcgc
     7561 cgaggcaatc aacatacccg tcaggggaat atctcgcgac aatgctaatt ggctggaaat
     7621 atagacaata ccaaaggcgt taataatatt agaggagaca ctttccgcca gcctcgcacc
     7681 cagtgccaat aaaatatttc gtggatgttt tttgaataat tcaatcaacg gaggaacgga
     7741 cttttcttta ttattcgtcg ttttttgctt ttgaaaatcc agcgtttctt cggtatgcag
     7801 gcggataaac agcccaacga tcaacatcac cgcagaaagc caaaacggaa tgcgccatcc
     7861 ccaggagaga aactgctccg aagtaagaaa atggttacag agagcaaaaa taccggttgc
     7921 cagcataata cccacagacg cagccgtttg cgggagtgaa cctaaaaatc cgcgtcggga
     7981 ttcgggcgct gattctatgg tcatcaatgc cgctccgcca tactcgccac caagaccaaa
     8041 gccctgaatt aaacgcagcg ccattaatat gagcggtgcc cagatcccaa tttcctgata
     8101 cgtcggaata aaaccaatca aaaatgtgga gcaaccgaca atagccagtg tccagataag
     8161 tgttattttt cgcccaattt tatcgccaaa atggccaaag acgataccac ctaaaggacg
     8221 ggtcaaaaaa ccaacgccaa aggtagcaaa ggcggctata agacctataa aaggatcgct
     8281 gtctggataa aacagcggtg caaataccag tcctgctgcc gtgccaaaaa taaagaagtc
     8341 ataccattcc attaacgcgc ctgcgaaact gcccataacg acacgtctca tggctttacc
     8401 ggaaagccct tcacgcggtg cgggtaactt ttcagacata attttcctct cctggtaggc
     8461 gagaaatctg ggatacgaac cactaaacgt gggcagtttg aattaatgcg catcggttta
     8521 agccgatgcg cattaattta tttcttcaat tcagccagaa cctctgcatc ctcagcatca
     8581 acatttgcac ccagtggttc ccaattaatc aatacgcagg tcaggaaccc caagaatggc
     8641 acgattgcca accagtaaaa cgcttcagtt ttcaacgccc cccacagcat cggccagaaa
     8701 atgagtcctg caattgcccc tgtgcgcata atagcgcggg caaatccgac cccagtaggg
     8761 cgaatagcgg gaggataaga gagtgtggca atggtcatgc ccagcccacc aggacccgct
     8821 gaatggaaga aaataattga tcccaataac cctaaagaca gccatggatt agtcgttgcc
     8881 agtgcgccta acgacagcaa tgctaaggtc acaacagcaa atccatacat cgactgtcgg
     8941 cgagtaccca gtcgttggag aataaacgag ccgatcatcc cgcctgtcac cccacaaagg
     9001 gcattcacga cggcagaacc cgtcaacgca ccagtaagcc cgccacttat tattcccgcc
     9061 aacgtaagag gaagataaac acctacggcg ttatattgcc acgcctgcat ggttgccacg
     9121 acacagccaa gaatggtgcg tttgcggtaa cgatcattaa ataagatgcc atatccacca
     9181 ctgtatttat tctctgcttt ttccgagaat ttcgcttctg ttgtactcga tgccgcaacg
     9241 cgagcctgaa caccataacg ttttcccaga atgtcgcacg cttcctggta acgcccgacg
     9301 cgtgcggccc acattgcgga ttcaccaata aagaagtaac gcaaaatcat gaagatgact
     9361 gcaaaaatag ctccgatgaa tattccgtaa cgccacaatt gttcttcagc gatacccgac
     9421 agaagcagcg gcaagagcac caaataaaca acagttgtcg aaacatacca ggccatttgc
     9481 cagaggttga ccgacgttcc ggttttcttc gacgttgaac cacgaagctc tgccacggca
     9541 ttggttgcca aagggaagtc gatgcctaaa ccaaagccca tgatgacgcg gcacaccagc
     9601 acccaccaga tactgggcgc aatagctacc gcagcagccc cgatggtgca cagccccatg
     9661 ccaataataa atgctcgctt ttgcccaaag cggtttatta ctcggttcgc taataaaccg
     9721 ccaattaacg cggcgattaa tactgacgca ttaacggttg cggcaaggcc tggagaaatg
     9781 ccaaattgag cggcaatgta tttattacca aaccccacca tcgccgcctg gtaggcatca
     9841 atcaatatgc cgcccaaagc aataaaaata acgccgatac tggtgttgat tgcagagttc
     9901 ttattaacaa tatcaattac gtcctgtggc cgactaattg aataagttgc aggactggtg
     9961 atttgactca tcgcagtgtc ctttttaatt gttgtagggt ggcgctgacg tgacgcagcg
    10021 ccagacagag atttagaaaa tcattccggc gcaaattgca gcatgacctt tatggagtca
    10081 ttttgctgcg cgcgttcgaa tgccgccgct gcatcctcaa tggcaaataa atcggtaacc
    10141 agttcactgg tatcaaaccg cccatccgcc agccattcga taacagcatc gaaatcttca
    10201 gggatgtaca tgccagagtt gagcaagtca cgctcgaaac gttgcatcat cggtaatggt
    10261 atttcacgcg gcccacttgg tacgcccatg cacacgacgg tgcctccggc atatacacgg
    10321 gtacatgcat cgttaagcgt ggcctgcgct gcaacgacat caatcgcccc cgtaaatcgc
    10381 acatctgcgg ccagctcacc tggtgcccaa acttctgctg cgcccagctt taacgccagc
    10441 gcgcgtttgg cagcgtcagg ttcaatcacg gtaatcttac ctgcccccat aatgcgcagt
    10501 gcctgcacga tggataagcc tatcgttccg gcaccaatca ccagtacgct ttccagagaa
    10561 gctttcggca ttcggttaac gcagtgacgg gcacaagccg ccggttcggc aaaggccaac
    10621 acttttagcg gtaaagaggc tggtgcgaca tggcaattac gcgcaggaac aatgtgttgt
    10681 gagcgagcaa agcccggtgc gcgaaaacca gcaacctgcg gtggttcaca aagattaaaa
    10741 cgtcctgctt tgcaggctcg gcattccatg caagccatga tgggatcgac cacaacatga
    10801 tcgcccggct gtacattttt aacgtcgctg ccaacttccg taacgcgcgc tgcaatttca
    10861 tgaccgggga cgacaggtgg tttagcaaac ggatgaccgc ctttcagaac atgcagatcc
    10921 gaaccgcata tcccatagaa cacaggcgca atccgcactt cgtgcggttt aagttcctgg
    10981 tgtggtactt caacatattg agtggtgact ttcccaatat ctgaaaataa aacctgggta
    11041 attttatcca ttgttattct ccagaatcga tatccatagg ggattagcgt tgcgcgatac
    11101 cttcatcgac aatttctgcc aaccgttgta aacgaggaac agagacatcg cgcagcatag
    11161 tttgtggatc ttgtgcacca acaaccgacg cccaaaccgc tcgtccagcc aggaagcctg
    11221 acgcaccacc tttcatggca atacttactg cacggccaaa aatatcggca tcaacgccag
    11281 aagaaagaat tacccacggc atcttcatct gatcgttaag ttgctgacag gcggcaagta
    11341 aggttttttc atcgcctttg ccccctaatg gcatctcggc tttgtaaaga tcagcctctg
    11401 ttccaccaag ctctgcggcg gcggcaacaa tggcgctttc acgatcaaag tcccaaccgc
    11461 gacgaggtgg acgcaccaca ggttcaataa tgctaaccag cccggcacta cggcagcgac
    11521 gaacaaattt atcaaccatt gccagacgtt cctctgcggg ttcatcttca cgccagagta
    11581 caagtaattt cattgcctta gcccccattt cacgggcttt atgcgggtcg acggacatat
    11641 ctatttcaac gctatcaacc gggatgccat tacctgggat aaaaagatcg gcagcgacaa
    11701 tcaggccgca agaattagca acagcgccct gctcgactat ttgctcaaga caaaattgtt
    11761 tatccgccag aacggcagaa gcataaggag aaaggattcg ggtcgcggca actttaaaat
    11821 cggtgagtac actgtcggcg atgggttttg gctgccctgc tgccgcaaac atcaagcgca
    11881 tggcctcacg ctgatcgaca gctaacatgg caaatccacc acctgggcgg gagatatctt
    11941 tcagcgtaaa tggggtgaag tgggatgaca tagaaactcc tgttaataga taaaattaaa
    12001 gaccagcctg ctggcgaacc tgatttagca gggcactcca gtcctgccgc cctttgccgt
    12061 cttgacttgc tgcagcgtaa taactttctg ctgcaatccc catattgagc ggtgcccccg
    12121 tctcccgagc gacgtcaact gcaatacgca gatctttcaa cgccagatcc accatgaagg
    12181 cgggtgaaag atcgcctttc agcactttgc aaggccaggt ggtcgtaaaa tgtcctttcc
    12241 ctgctgccgt accactcatc accttaattg cgacatcaag atttaatccc agtgactcac
    12301 ataacacagc ggcctcggag gacagcgcat taagcgcaat gctcatatag ttgttaatta
    12361 gcttcacgcg gatccccatt cctgggccgc cagcctcgac cagctcatta cccatgcaca
    12421 tgagaatcgg gcgtgcacgt gcaatctgct catcagtgcc acccgctaaa atcagcaatg
    12481 tgccatcaat cgcattcacg gacgttctgc ccacgggggc gtccatcata ctgatgcctt
    12541 tttcttgtaa ctcccgtatt aatgcatcgg tctgtaaggg atgaatagtc gacatatcaa
    12601 tcaataacgc gtcagatgaa acagcctcac agacgccttt ttcccccaac agaacctgtc
    12661 gaacaatgtc accattggga agcatagtga tgataaattc agcgcctgct gccgcttccg
    12721 ctggggtttg tgccgcagtg gccccctgcg taactagcgc atcgacggcc tgtgcattga
    12781 catcaaatac ctgcaatgaa tgcccttttt gcaatagatt gttagccatt gggctaccca
    12841 tctgccccag cccgagaaat gcaatggttg ccatgaatac tctcctgtct gacattgaca
    12901 aaataatgac aattgaaaat tcgttttcgt ctgtttttga gcatatttgt aatttatgtt
    12961 caaaaacacg acacgcatca ctaatttgat catttgtgtc atttaaattg accgaagacg
    13021 acaaaattaa acaggacaag acaaatatga ccaaaattgc atgcgtgggt atcacagttc
    13081 aggacaggat ttattctttg ctcactctgc cggatggcgg cggaaaatat caggcaaatc
    13141 attatcttga gattggtggc ggcccggcag caacagccgc tgttgcaatc gcaaagcttg
    13201 gcgtagaggt tgatttcatc ggtcgcgtcg gcgatgacag ttgtggaaat accttacttg
    13261 cagaacttga gggttggggc gtcaataccg cattttgccg ccgatatcct aacgcccgtt
    13321 catcgcaatc agcgatcctc gttgatcagc atggcgaacg gattatcgtt aattatccaa
    13381 gtcccgatct cggtactgat gccgaatggt tagaagcgat cgatttctct cgttatgatc
    13441 tgattctggc cgatgttcgc tggcatagcg gtactgaaaa agcattctcg ctagcgcgtc
    13501 ttgcgggtgt gacaacgctt ttagacgccg atatgacacc gcaggatatt tctccacttg
    13561 tcgcgcttgc cgatcacgcc gtgttctcaa caccagggct gaaacgcatg actggcctgc
    13621 agtcacctga agaaggtttg ttccaggcaa cgacataaac agcaggtaaa gtgtatgtta
    13681 cgctgggttc tgaaggatcg ttgtggattg aagatggtca tttgtgtcaa caggaggcat
    13741 tttctgtcaa tgtggttgat accactggag caggcgatgt ttttcacggc gcactcgcgg
    13801 tagcgttggc agaaaaaatg ccaacaaaag aggctattcg ttttgccagt gcggttgccg
    13861 cgatgaaatg tacccagcct ggcggtcggg ccggcattcc taatcgtgag caaaccgaat
    13921 catttttgtc actttatgcg taaaatggta aacatctacc gaggaggggc aagggagtat
    13981 tcatgagtat catcgaagtg acaggtaatc cacgccatga ccaactcgtt catctaatcg
    14041 cagaacgcgg ttatatgaat attgaagaac tggcacaact gctggatgtt tcaacgcaaa
    14101 ccgtgcgccg ggatatccga aaactcagtg agcagggctt gatcacccgt catcatggag
    14161 gggccgggcg cgtctccagc gtcatgaata ccgcttttga gcaacgggaa ctttcgctca
    14221 ccgccgagaa acgggcgatc gctgaggcag tcgccgatta ccttcccgaa cgctgtaccg
    14281 tctttatcac catcggtaca accgtagaag ccgttgccag ggcattactc aaccggcgtg
    14341 atttacgcat tatcactaac agcctacgtg tggcacagat tctttataag aatcaggata
    14401 ttgaagtgat ggtgccggga ggcactttac gcgctcataa cggcgggatt atcggcccag
    14461 gagccgtgga ttttattgaa ggcttccgcg cagattattt aatcaccagt ataggtgcca
    14521 ttgaacatga cggcacccta ctggaatttg atgtaaatga agcgttagtc gcgagaacga
    14581 tgattaaaca tgcgcggaat acattgttag tggccgatca tacgaagttt gccgcgtctg
    14641 ctgccgtttc aattggcaat gcacggaatg tcagggcttt ttttacagat gccccgcctc
    14701 ccaattcttt ctgccagttg ttaagtgaag agaatgttga actggtggtt gccgagcagg
    14761 aagtttcctg aaattgaaac ttcgaaaaat ccgctgggga ttcagcggat tttattttgc
    14821 cggatgcggc ctacggttga tacggttttc gttggccgga ttaagcacat ccggccatta
    14881 attaagcttc agccgccagc atctctttct tacgttggct aatacgctta accagcatta
    14941 cggaaaccac cgcaccacag aatgcgaagc aggccataat gccaaacacg attttgtagc
    15001 caataatccc cggatttaaa tccagaatgt agccatacag actgaagcag aacatcgccg
    15061 gagcgtaacc aataaagcta cccaacgcca tcgccgcgcc tgttttattt tcagcaattt
    15121 ttgcttcgcc gataggtgca aaaaatacgg ctcgctgtgt aaagactatc gcgccaaagc
    15181 ccagcgtaca tgccatccct aaatagaccg gcatactttc gtgcggcagc ataatcaaca
    15241 gtacgagcgc agcggtactg atgataaagg tgtagcatag atatttactc ggcgatttca
    15301 ggatcttatc tgaaatcatg ccaccaatcg gtccaccaat cattttcaga cagtattggt
    15361 taatgatgcc gtaagccccc accagcgcaa cgggcaatag atagatgttt ttcaggaatg
    15421 gaatgaagaa tgtcaggcca cagtaaaccg cgtaaacgaa gaagacgtta aaagcgataa
    15481 gccagatggt tttgtctctc agcaccgagg tcatactggt gttttgcgat gctccgtctt
    15541 cttttttcac ctcaacggac ggtgcctctt ctttgtcatt caggacaaag aaaataataa
    15601 tgcctacggc aatcacaata agggaataga accagatgcc tgctttaaaa cccaataagc
    15661 cactgccaaa ccaggtaaat actgccaacg cagaaaatgc caccacggta tcgacaatgc
    15721 cacgccctgt ttcgaagaag ccaaacaacc gaccttgttg ttcgctattg cccaatcgac
    15781 ttaccgactt gagcaagacc ggccagttca tcatgtcgca agtaacgcca aatagcgccc
    15841 agacaaagag gatgccccaa taccccggca ttgtcgtcaa ataaacaccc agtaatcctg
    15901 tcgcaatgag tgagaatgac atggtgtatc tgcgcggtag tttatcggca aaataaatag
    15961 acagaaaaaa acccactgtc gtgacaaatg agtttaccga catagcatta ccaatttgac
    16021 cattggtcaa atggaaatat tcctgcatcg ggatataaaa cgcatctttc agcgacggta
    16081 atttataaat tgtaccgcca cacaatgtta atagactaaa taacgcccat ttctttttcg
    16141 tgagcataac atcccctaag cgatttttaa aatcactgtt ttaaaggcaa taagataccg
    16201 accattcata tggaatggtt ttattaactg cgtatgagtt aaaacttatt agtggttcag
    16261 aagatggttt gcctcttgtt tgatttgttg tagtacgtta cgcacatgat ttatctgatt
    16321 aattgcatct tctttttctt tacgtaaccg cgcatccagt aaatgatttt ctggcaatgg
    16381 tgtttcactc atctggcgat aagggatcca cggattatca tcttcgtctt caaagcggaa
    16441 cgccggcgca tagtaatcaa cctcttcttc caggccatat gccgtcacct gcggctcatc
    16501 atcacccagg catataaggt gcgttaataa cgctttgaac ggcatatcac cctgacctga
    16561 aatacaggct ttatgaccca ggccacccgg ttctttaacg atcaaggcat ctttgatatg
    16621 gacctgggta atatgcggcg ccatcgtttt taaagcgtcg atgggatgct catttgcatt
    16681 gatcatgttc gcaaaatcaa acagtaagga aagagattcc atctcacttt ctttgaccag
    16741 cgacaccagc tcatggcttt ttaaatcttc atgctgttcg atcgtaaaag tcaggccgct
    16801 gtcctgatac gtttcccgta cataggcaat gtcgttagcg atgatcgata atacgtcgcg
    16861 caaattacct tcataacgtg gataaaaacg tacggacgat gccccagttt tcaacgcaat
    16921 ggcgacggct tcgtcaatag atgccttatc tgaggcgctg gtttcaatat gaatatcaag
    16981 gttcagacgg cgggctttat ctccaaaggc agagagttct ttatcgtcca tattaccaag
    17041 agaaaaacgc tcgccatcca gaacatggat tttgacgcca cgtagattat tttcacttgc
    17101 gatatcaagt aaatcagcag gcaacactct ttccagccgc atatttaaat gaaaggcata
    17161 agcatgtaaa taaagaggaa gagtgtcgac acgttgtaga atctttcttg cgttatttat
    17221 tgtcaccata aaacacctac agattaatga tgaggtctac ggcctcaaat cgccaatatt
    17281 caatatctat ctggacaata ttgccttgct ggtcggcgcg atgcttttca attaagatcg
    17341 ctggcatacc cgcagaaccg ccaagatact tgctgatatc gcccggcatt ctgacgggtt
    17401 tgaaaatcaa tttttttact accgtctctt tttggtagca cttttcccag actgcagaaa
    17461 atgagtggtt ttcaagttgt tcaataaaac ccggagcaac ttcaggatta atatatgttt
    17521 cgtgatagaa aactttatgt ccttccagcg ccccccagcc ggtgatgcga tagagttcag
    17581 ttgatggtgt cactgcaatc agtggcgcga gcgtctcggg aatatcgctg gtacggtttt
    17641 tctcggtaaa cccccaggaa ggctctcgtc cttgctcaat tgcggcccgc tgaaagctcg
    17701 ccgacaactc cggactgtaa ttaaatcgcg gctgggtcac gaaccacccc ttacgatcct
    17761 tacggaagat tttggattct gcctcgaggt tcaacaacgc ctggcgcagc gtcatacgtt
    17821 taatgcccag cagttctccc agctctcttt ctgaagggag ttttccaccc ggcgtggtga
    17881 tgccatcttt caaccaccga tccagtttct cttttgcatt ttctacggtg gattgattct
    17941 ccgccattgt ttaatccttt tggtttaaac cagatgaagc atcttacgct tacgccaaaa
    18001 aagaaagcaa ttaacggcgt atgactttgc agataattgt gacaaagctc tctattgacg
    18061 taatcccaga cttataaggg agatactcgc attggtgtgc gctcacgaca ggtaaaaaaa
    18121 aacctgccag cgatggcagg ttttttataa ctaaaaaacg aaattaatcg tctttcggtg
    18181 cgcggttggc gcggcgacga tcgttttccg tcaggtgacg tttacgaata cggatagagg
    18241 tcggagtcac ttctaccagt tcgtcatcat cgatgaactc cagagcttgt tccagagtca
    18301 tgcggatagg cggaaccaga acaacggctt cgtcagtacc ggaagcacgc atgttggtca
    18361 gtttcttacc ggtcaggcag tttaccgtca ggtcgttaga gcggctatga ataccgataa
    18421 tctgaccttc gtaaacttct gcaccgtgac cgaggaacag cttaccgcga tcctgcagac
    18481 cgaacagcgc gaacgcgacc gctttaccct gaccgttaga gatcagtacg ccgttctgac
    18541 gctgacccac ttcacccgga cgtacgtcgt cgtagtggct gaaggtggag tacagcagac
    18601 cagtaccaga agtcatggtc atgaactcag aacggaagcc aatcagacca cggcttggga
    18661 tcacgtagtc gagacgtacg cggcctttac cgtctggatt catgtttttc aggtcgcctt
    18721 tacgttcgcc cagcgcctgc attacagaac cctgatgctg ttcttcaacg tccagagtca
    18781 cgttttcata cggctcttgt ttacgaccgt cgatttcacg gaagataact ttcggacggg
    18841 ataccgccag ttcgaaacct tcacgacgca tgttttcgat cagaacagac aggtgcagtt
    18901 cgccacgacc agaaacgcgg aacgcatcgg cgtcttcggt ttcttctacg cgcagcgcaa
    18961 cgttgtgtac cagttctttg ttcagacgat ccaggatctg acgagacgtt acgaacttac
    19021 cttctttacc gcagaacggc gaggtgttaa cgcagaagaa catagaaacg gtcggctcat
    19081 caacggagag tgccggcagc gcttcaacgt tttgcgtgtc gcaaacggtg tcagaaatgt
    19141 tcagttcgcc aaggcccgtg atcgcaacga tatcgccagc ttccgccaga tcggtttcga
    19201 tacgttccag accgaggtgg cccagcactt taccgacttt cgcgttgcgg gttttgcctt
    19261 cgctatcgat gatagtgacc tgctggttcg gcttcacttt accgcgcttg atgcggccaa
    19321 tgccgataac gccaacatag ctgttgtaat cgagctgaga aatctgcatc tggaacggac
    19381 cgtcaaggtc aacgtccggc gcaggaacgt ggtcaacaat cgcctggtac agcggggtca
    19441 tgtcttccgc catatcttcg tggtccagac ccgcgatacc gttcagcgca gaagcgtaaa
    19501 cgatcgggaa gtccagctgc tcgtcggtcg cgtcgaggtt aacgaacaga tcgaatacct
    19561 gatccacaac ccaatcagga cgcgcgccag ggcggtcaac tttgttgata acaacaatcg
    19621 gcttcaggcc gtaagcaaac gcttttttgg ttacgaagcg cgtttgcggc atcgggccgt
    19681 caaatgcgtc aaccaccagc agcactgagt ctaccatgga cattacacgt tcaacttcac
    19741 caccgaagtc ggcgtgcccc ggggtatcaa cgatgttgat acggtaatca ttccatttga
    19801 tagcggtgtt tttcgcgagg atggtaatcc cacgctcttt ctccaaatcg ttggagtcca
    19861 tcacgcgctc ttgggtttcg gcacgagagt cgaacgtacc ggattgttgg agcagcttgt
    19921 ctaccagggt ggttttacca tggtctacgt gcgcgatgat ggcgatatta cgcaattttt
    19981 cgatcacaac tttgcctcag gcattagaaa tagcgcgtta ttgtacacgg attaatcgca
    20041 ctacaaaaca ggatcacaaa catcctccgc aaacaagtat tgcagagtcc ctttgtgatc
    20101 gctttcacgg agcataaaaa gggttatcca aaggtcattg caccaacatg gtgcttaatg
    20161 tttccattga agcactatat tggtgcaaca ttcacatcgt ggtgcagccc ttttgcacga
    20221 tggtgcgcat gataacgcct tttaggggca atttaaaagt tggcacagat ttcgctttat
    20281 cttttttacg gcgacacggc caaaataatt gcagatttcg ttaccacgac gaccatgacc
    20341 aatccaggag agttaaagta tgtccgctga acacgtactg acgatgctga acgagcacga
    20401 agtgaagttt gttgatttgc gcttcaccga tactaaaggt aaagaacagc acgtcactat
    20461 ccctgctcat caggtgaatg ctgaattctt cgaagaaggc aaaatgtttg acggctcctc
    20521 gattggcggc tggaaaggca ttaacgagtc cgacatggtg ctgatgccag acgcatccac
    20581 cgcagtgatt gacccgttct tcgccgactc caccctgatt atccgttgcg acatccttga
    20641 acctggcacc ctgcaaggct atgaccgtga cccgcgctcc attgcgaagc gcgccgaaga
    20701 ttacctgcgt tccactggca ttgccgacac cgtactgttc gggccagaac ctgaattctt
    20761 cctgttcgat gacatccgtt tcggatcatc tatctccggt tcccacgttg ctatcgacga
    20821 tatcgaaggc gcatggaact cctccaccca atacgaaggt ggtaacaaag gtcaccgtcc
    20881 ggcagtgaaa ggcggttact tcccggttcc accggtagac tcggctcagg atattcgttc
    20941 tgaaatgtgt ctggtgatgg aacagatggg tctggtggtt gaagcccatc accacgaagt
    21001 agcgactgct ggtcagaacg aagtggctac ccgcttcaat accatgacca aaaaagctga
    21061 cgaaattcag atctacaaat atgttgtgca caacgtagcg caccgcttcg gtaaaaccgc
    21121 gacctttatg ccaaaaccga tgttcggtga taacggctcc ggtatgcact gccacatgtc
    21181 tctgtctaaa aacggcgtta acctgttcgc aggcgacaaa tacgcaggtc tgtctgagca
    21241 ggcgctgtac tacattggcg gcgtaatcaa acacgctaaa gcgattaacg ccctggcaaa
    21301 cccgaccacc aactcttata agcgtctggt cccgggctat gaagcaccgg taatgctggc
    21361 ttactctgcg cgtaaccgtt ctgcgtctat ccgtattccg gtggtttctt ctccgaaagc
    21421 acgtcgtatc gaagtacgtt tcccggatcc ggcagctaac ccgtacctgt gctttgctgc
    21481 cctgctgatg gccggtcttg atggtatcaa gaacaagatc catccgggcg aagccatgga
    21541 caaaaacctg tatgacctgc cgccagaaga agcgaaagag atcccacagg ttgcaggctc
    21601 tctggaagaa gcactgaacg aactggatct ggaccgcgag ttcctgaaag ccggtggcgt
    21661 gttcactgac gaagcaattg atgcgtacat cgctctgcgt cgcgaagaag atgaccgcgt
    21721 gcgtatgact ccgcatccgg tagagtttga gctgtactac agcgtctaat agttgaagtt
    21781 gtactacccg gcgcaacaac gccggaggtt caaaccaggc ccatctggcg taattgttgc
    21841 agccagtttg aacacggaca gcgcgcagaa cccggagcgt acactggtac gtgaggagtt
    21901 cgagcactgc ccaggttcaa aatggcaaat aaaatagcct gatgggactg gtttttagtt
    21961 gccgtggaaa tttcagccca tctctgcatg ggcttttttc tccgtcaatt ctctgatgct
    22021 tcgcgctttt tatccgtaaa aagctataat gcactaaaat ggtgcaacct gttcaggaga
    22081 ctgctttatg gcaacaggca cgcagcccga tgctgggcag atcctcaact cgctgattaa
    22141 cagtattttg ttaatcgatg acaacctggc gatccattac gccaaccctg ccgcgcaaca
    22201 actgctcgcc caaagctccc gcaaattgtt tggtacaccg ttaccggaac tgttgagcta
    22261 cttctcatta aatatcgagc tgatgcaaga aagtctggag gcggggcaag gttttaccga
    22321 taacgaagtg acgctggtca tcgacgggcg ctcgcatatc ctttctgtga cggcccagcg
    22381 tatgccggac ggcatgatcc tgctggagat ggctccgatg gataaccagc gccgcttaag
    22441 tcaggaacag ctacagcacg cccagcaggt tgctgcccgt gatttagtgc gcggcctggc
    22501 acatgagatt aaaaatccgc ttggcggttt acgtggcgcg gcgcagctgc tcagcaaagc
    22561 gttacctgac ccatcactac tcgaatatac caaagtgatt atcgaacagg cggaccggct
    22621 gcgaaatctg gtcgaccgtc tgttggggcc gcagctgccc ggtacgcgcg ttaccgaaag
    22681 tattcacaaa gtggctgaac gcgtggtaac gctggtgtcg atggaactgc cggacaacgt
    22741 gcggttgatt cgtgattacg atcccagcct gccggaactg gcgcacgacc cggatcaaat
    22801 tgaacaggtc ttgctgaata ttgtgcgcaa tgcgctacag gcgctggggc cggaaggcgg
    22861 tgaaatcatt ctgcgtaccc gcaccgcgtt tcaactgacc ttacacggcg agcgctaccg
    22921 gctggcggcg cggattgatg tggaagataa cgggccgggc attccgcctc atttgcagga
    22981 tacgctgttt tacccgatgg tcagcggccg cgaaggtggc accgggcttg gcttatccat
    23041 cgctcgtaat ttgattgatc agcattcagg caaaattgaa tttaccagtt ggccagggca
    23101 taccgagttc tcggtttacc tgcctatcag gaaataaagg tgacgtttat gcaacgaggg
    23161 atagtctggg tagtcgatga cgatagttcc atccgttggg tgcttgaacg tgcgctcgct
    23221 ggggcaggtt taacctgtac gacgtttgag aacggcgcag aagtgctgga ggcgctggcg
    23281 agcaaaacgc cggatgtgct gctttcagat atccgtatgc cgggaatgga cgggctggcg
    23341 ctgctcaagc agattaaaca gcgccatcca atgcttccgg tcatcattat gaccgcacat
    23401 tccgatctgg atgctgccgt cagcgcctat caacaagggg cgtttgatta tctgcccaaa
    23461 ccgtttgata tcgacgaagc agtggcgctg gttgagcgcg ctatcagtca ttaccaggaa
    23521 cagcagcagc cgcgtaatgt tcagcttaac ggcccaacga ccgatatcat cggcgaagcg
    23581 ccagccatgc aggacgtgtt ccgtattatc ggtcggcttt cgcgttcttc tattagcgtg
    23641 ctgattaacg gcgaatccgg caccggtaaa gaactggtcg ctcatgccct gcatcgccac
    23701 agtccgcgcg ccaaagcgcc gtttatcgcg ctgaatatgg cagctatccc aaaagatttg
    23761 atcgaatcag aactgtttgg ccacgagaaa ggcgcgttta ctggcgcgaa taccattcgt
    23821 caggggcgtt ttgaacaggc cgatggcggt acattattcc tcgacgaaat tggtgatatg
    23881 ccgctggatg tgcagacgcg tttgctgcgc gtgctggcag acggtcagtt ttaccgcgtt
    23941 ggcggctatg cgccggtgaa agtggatgtg cggattatcg ctgccactca ccagaatctc
    24001 gaacagcgag tgcaggaagg taagttccgt gaggatctgt tccaccgcct gaacgttatc
    24061 cgcgttcatc tgccgccgct gcgcgaacgt cgggaagata ttccccgtct ggcgcgccat
    24121 tttttacagg ttgccgcgcg cgaactgggc gtagaagcga agttactgca tccggaaacc
    24181 gaagctgctc tgacgcgtct ggcgtggcca ggcaacgtgc gccagctgga aaacacctgc
    24241 cgctggctaa cggtgatggc cgccgggcag gaagtgttga ttcaggattt gcccggcgaa
    24301 ctgtttgaat caacggttgc ggagagtact tcgcaaatgc aaccggacag ctgggcgacg
    24361 cttcttgcgc agtgggcaga cagagcgctg cgttccggtc atcaaaatct gctttccgaa
    24421 gcgcagccag agctggagcg gacgttactg acgaccgcgt tgcgacatac gcaggggcat
    24481 aaacaggaag cggcgcggct actcggctgg ggccgcaaca ccctgacgcg taagttaaaa
    24541 gagctgggga tggagtgatt cacagcttgt gtgtaaagat tgattattga gcgcaaattg
    24601 ctggtatttt acgctttact gttccgataa gttcagtatg atcttgcccg gaaaacgggg
    24661 agagtcatta tgctggaatc aataattaat ctggtatcga gtggcgcagt tgacagccac
    24721 acaccgcaaa ctgccgttgc tgccgtgctg tgtgccgcaa tgattgggct gtttagttga
    24781 gtttcagatt gctgatgaag tgctctttgt tcatgccgga tgcggcgtga acgccttatc
    24841 cggcctacaa aagcaatatt ttcaacaggt tttgtaggcc tgataagcgt agcgcatcag
    24901 gcaatcttgt tctcagcaca ttgatggcgc tttcgttttc tactttgtaa acgaagcgcc
    24961 attcactatt taaatcaccc gtgagaactg ctgcatccgc gctttctggc gcagataggt
    25021 atcaaagcac atgcaaatgt tgcggatcag caagcgacct ttcgccgtca cctgtattcc
    25081 cttctcatcc acatccacca gcccatcttt tgctaacggg gcgagcagct tgagatcttc
    25141 cgcaaagtaa tcagcgaagt gcaaatccca ctgtttctca ataggggcgt aatccagacg
    25201 gaagttgcag atgagcgact taatcacatc gcggcgaata cagtcatcac gcgttagcgc
    25261 aataccacgc cacagcgcat tgccttgttc atccacttgc tgatagtact gcttcaactc
    25321 tttctggttc tgcgcgtagc agtcgccaat catgctgatg gcggaaacgc ccatccccag
    25381 cagatcggta tcgccctgag tggtgtagcc ctggaagtta cgatgcagca cgccttcacg
    25441 ctgggccacc gccagctcgt catccggacg ggcaaagtga tccataccga taaactgata
    25501 gcccgattgc gtcaggaagg cgatggtttc ctgcaggata tcgagttttt gctgcggact
    25561 cggcaggtca gcatctttga ttttgcgctg agcagcaaaa atggtcggca gatgcgcgta
    25621 gttaaagaca ctcagacgat cggggttcag ctccgccaca cgtttcaggg taaaggcgaa
    25681 actctccggc gtctgtttcg gcaggccgta aatcaggtcg atgttggtgg aggtaaaacc
    25741 aatctcacgc gcatggttaa gcagtgcaaa gatgaactct tcatcctgct cgcggttaac
    25801 cagacgttgc acttctttgt tgaagtcctg cacgcccatg ctcaggcgat taaagccttc
    25861 ggcgcgtaaa tgatcgagta catccagttc gatttcccgc ggatcgactt cgatcgaaat
    25921 ctccgcatcg gcattgaact ggaagttttc gcgcagcagc ttcatcaggc ggctgatttg
    25981 cgctttattc agatacgtcg gcgttccgcc gccccagtgc aattggctga cgtgacgccc
    26041 ggcgaacagc ggtgcacgat ggacgatttc ttgctccagc gcgtccagat actgatcggc
    26101 cttgtgctgc tggcgagtaa caatcttatt gcaaccgcag aagtaacaaa gcttatggca
    26161 gaacgggata tgtacgtaga gagataatgg acgctcagga tagcgcgcca cggcttgtaa
    26221 aaacgcctgt tcgccgaagt cttctgaaaa ctccagcgcg gtcgggtacg aggtgtatcg
    26281 tggcccggaa tagttatatt tctggatcag ggccagatcc cagtcgattt gctgtacaga
    26341 catgctcact ccttccgatg gcgtctctgg cggctacggc gtaccggcgg atgcccgttt
    26401 cgggtcatca gccggttgcg cagccacttt tgtcgccgcg acaaccgctg tagtttaacg
    26461 aataaccaca ccagataaca tataaccgga agggttatca gcagaacggg aaggcccacg
    26521 gcggcaatcc gttagttgcc ccgcagcaga cgcatcatgt cttcttgctt ctcgtcttct
    26581 tcctcttctt cgtcatcgtc ataagagagg ccgagtttct gcatcaactc atcaatacga
    26641 tccagtttag catccaccca ggattgctct tcggcgctca gggtttcgcc tgcttccaga
    26701 cgttccagca gcgcatccag acgctcatcc gtttccagta attccaactc ctcctgcggt
    26761 gaaagcatag gtttctcact cttcggtttg tgctgtttgg tgactttttc agtcacgccc
    26821 aatggaatag gggttttact gccaatacgt ggatcttttg gtgcgttctg gcctttgctg
    26881 cctgacgtgg tgtttccgcc cgctgcgcgg ctgcccggcg catgaccacg acgttttttc
    26941 tgacgcttgc gatcacgagc ttcctgatcc agctcctcgc gtgtttttcg acgcgctttt
    27001 gcatgacctt tgctgcgtga gtttgaagat gatggtttca taatggttcg tcttttagcc
    27061 gttttattca gtatagattt gcggcggaat ctaacagaaa gcaagcaaag aaaaaaggcg
    27121 acagattact ctgtcgcctt ttttcctgac tcataaccct taacgggtct tacaatcctt
    27181 gcaggccgcc aacacaccct gtttttcctt tagccggaac aatccgtgtt gattccattt
    27241 ccgtttaatt acgtctccgg acgtttgtct tcctgacaat cctctgtctt cgcctcctgg
    27301 cgctcctgac cctcatcctg agtcattgtt cctgttagcg tcctcgctct atgggtgcta
    27361 ctttacgcct ttgctttaaa caaacaatca accgccatca gtatttgcac attttcagag
    27421 ttttatataa aagcaattca ctgatttata tagcttattt gacatgcatc gcagaatttt
    27481 ctctgctatt tcgcataaag actatcggca atgtctcaca aagcgtatgg cttttactta
    27541 caacgctcac gccgaaaaat ccgcctttta ttatggttca ggtataatcg ccacaaagct
    27601 ttgactgacg gagacgaccg ctttgactaa tttgaattat caacagacgc attttgtgat
    27661 gagtgcgcct gatattcgcc acctaccttc cgataccgga attgaagtgg cttttgcagg
    27721 ccgttccaac gcaggtaaat ccagcgcgct gaacacgctg actaaccaga aaagcctggc
    27781 tcgtacctca aaaaccccag ggcgcaccca gcttatcaac ctgtttgaag tggctgacgg
    27841 caagcgtctg gttgacttgc ctgggtacgg ttatgcggaa gtcccggaag agatgaagcg
    27901 caaatggcag cgtgcgctcg gcgaatacct cgaaaaacgt cagagcctgc aaggtctggt
    27961 ggtgctaatg gatattcgcc atccgctgaa agatttggat cagcagatga ttgagtgggc
    28021 ggtagacagc aatatcgccg ttctggtgct gctgaccaaa gcggacaaac tggcaagcgg
    28081 cgcacgtaaa gcgcaattga atatggtgcg tgaagctgta ctggcgttta acggtgatgt
    28141 gcaggttgaa acgttttctt cgttgaagaa acaaggcgtg gacaagctgc ggcagaaact
    28201 ggatacctgg tttagcgaga tgcagcctgt agaagaaacg caggacggcg aataattttc
    28261 ttgccttaat gcttgtgccg gatgtggcgt atccggcccg taaattcaat agattacaat
    28321 tctaccgttg gcctgataaa catagcgcat caggcattac ggatcttttc tttcgcccaa
    28381 taaaaaacgc cccagtcatt actgactggg gcggctaaaa tattcagcca aatccgatta
    28441 cgtgaagtaa aaggtctgaa agatagaaca tcttacctct gtaccctacg cgactaactt
    28501 tactcttttt tgttcagttc agaaagcact ttttgtaatt ttttttcagt tgttgcatag
    28561 gaatttctaa tagccatcac aaaacgtgac agcttatgtt gcttacttac gaaaaaaggg
    28621 catgttcagg cgaatcttag tgcgcctgct cccagttttc gccactcccc acttccacca
    28681 gcaacggcac atccagacgg gtacagtttt ccatcagttg atgaatctgc ttcgcgacgg
    28741 catcaacatc atctttatga acttcaaata ccagttcatc gtgtacctgc atgatcatac
    28801 gtacacgcgg ttgctcagcc tgtaaccacg catcaacggc aatcatcgcc cgtttgataa
    28861 tgtcggcggc ggttccctgc attggcgcgt taatggctgc acgttcagcc gctgcacgac
    28921 gagcaccatt gctggatttg atatccggca gatacagacg gcgtccgtcc agcgtttcaa
    28981 cgtagccctg ctctttcgcc tgagcacggg tgcgttccat atactccagc acgccagggt
    29041 agcgttcgaa gtaaaggtcc atgtacttct gcgcttcttt acgtggaatg ttcaattgcc
    29101 gcgccagacc gaaagcactc atgccataaa tcagaccaaa gttgatcgct ttcgcgctac
    29161 ggcgttgctc gctggtgacg gtttccagtg gcaaaccaaa cacttctgcc gccgttgccc
    29221 ggtggatatc ttttccttcc gcgaatgcgg tcagcaagcc tttgtcacgc gagagatgcg
    29281 ccataatgcg cagttcaatc tgcgagtagt ccgctgagac aatcacataa tcctctggcg
    29341 caataaacgc ctggcggata cgacgacctt cttcattacg caccggaatg ttttgcaggt
    29401 taggatcggt tgacgataaa cgtcccgttg cagttactgc ctggtgataa gaggtatgca
    29461 cacgcccggt tttcgggttg atcatcagcg gcagcttgtc ggtgtaagtc gactttagct
    29521 tcgccagacc acgatactcc agaatcactt ttggcaacgg atagtccagc gccagttctt
    29581 ccagtacctc ttccgacgtt gacggcgcgc cacccggcgt tttcttcagc ggtttaatgc
    29641 cctgtttttc aaacagaatg gtttgtaact gcttggtgga agaaaggtta aattcctcac
    29701 ctgcaatttc atgcgctttc ttttccagct cagccagacg aagggtgagc tcttcagaat
    29761 gattgtgcag cactttcgga tcgatcttca caccgttacg ttcaatgcgt gaaagcaccg
    29821 gcaccagcgg catttcgata ttctcgaaga cgttcaacgg ccctttgtgt ttttgcagat
    29881 ctggccacat tttcagatgc aactgcaagg tgacatctgc atcttcggcg gcgtaacgtc
    29941 cggcttcttc gagggcaatc tggttaaagg tcagttgatt tttgccttta cccgcaatct
    30001 cttcaaaagt gatggttttg tgcttcaacc aacgttccgc gaggctgtcc atatcgtgac
    30061 gcccggcaac gctattgaga atgtaggact ccagcatggt atcaaacgca atcccacgca
    30121 gctcaatgcc gtaattcgcc agaataccgc gatcgtattt caggttttgc ccgaccttca
    30181 gcgccttttc atcttccagc agcggtttta gcaactcgag tgcacgctcg cgagagattt
    30241 gatcgggcgc gtcaagataa tcatgagcaa ccggaatata tgccgctacg cctggctcga
    30301 tagcaaaaga aagcccgacc aggttagcag agatgttatc aaggctgtcg gtttcggtat
    30361 caaatgcaaa taccggcgct ttttccagct tcgcaatcca cgctttcagt gtttcttcat
    30421 caaggatggt gacgtagttg tcataagaaa tcaccgttgc cgtcacttct ggtgcttcgt
    30481 ctgcaacact ggtttcctgt ggcttcgcgg ctggttttgc ccctttggcc tgtaaccatt
    30541 tgcccgcttc gacatcagca gtccagcgtt tgaactcata ctttttgaac agccccaaca
    30601 actcttccgc tgccggttgc tgcacttcca gttgttcaca ggtcagctcc agttcaacgt
    30661 cggttttaat cgtcgccagc tggtatgaga gataagcaac ttctttgttt tgctcgagct
    30721 tcgctgccat tgttttcgcg ccacggaagc tcaacccagc aattttttct ggctcggcat
    30781 acagcgtatc cagtccgcca agaccttgca gcaatgcctg cgcggttttt tcaccgacgc
    30841 ccggtacgcc aggaatgtta tcagaggagt cacccatcag cgccaggaaa tcgatgatca
    30901 gttctggcgg cacgccgtac ttattcacca cctcttccgg tccgaggatg gtattcgtca
    30961 tggtattgat aagcgtaata tttggcgtca ccagctgcgc catatcttta tcgccagtgc
    31021 tgatcagcac cggacgcccg gctttttcgg cttcgcgcgc cagagtaccg ataacgtcgt
    31081 ccgcttctac gccagaaacc gccagcagcg gcagtcccat cgctttaacc atcgcgtgca
    31141 agggttcgat ttgtgcacgc agatcgtccg gcattggcgg gcgatgtgat ttgtaatgtt
    31201 caaacagttc atcacgaaag gtttttccct tggcgtcaaa gaccaccgct gcatgcgtcg
    31261 gtttatattg catgatcaga ctgcgcagca tgttgaggac accatacatc gcaccggtcg
    31321 gctcgcctgc gctgttagtc agcgggggaa acgcgtgata tgcgcgataa agataagatg
    31381 aaccatctac aaggataagt ggattttggg ggatctgaac cataatgtcc gtgcctgttt
    31441 atcagattat gggtaaagga tgccacagac aggatgaaaa catgagtttt tcaccgcttt
    31501 tcgtgaaaat cttttgtaga tcttctggat cgctcgcaaa tcgtcatgtg gataactttg
    31561 tgcataaatt atttacatcg cttttaattt attatcatgc tttttacagc atgatgtttg
    31621 tctttgtttt tcatgcagtt aaaaataaat ccatccctaa taattgagat aatgggattt
    31681 tttctaatgt ggatagatat gaattatttt tctccttaag gatcatccgt tatttgggtc
    31741 gttttctgct aaaatctgcg ccttgcgctt ctttagcgca ctgtctatgg ctaacctttt
    31801 gaataaattc attatgacga gaatactcgc tgcgataacc cttttgctga gtatcgtatt
    31861 gaccattttg gtcactattt tttgttccgt cccgatcatc attgccggga ttgtaaaact
    31921 attgctacct gtgccagtca tttggcgaaa ggtttcgcgt ttttgtgatt ttatgatgta
    31981 ttgctggtgt gaaggtctgg cggtattact gcatctgaac ccacacctgc aatgggaagt
    32041 tcacgggctg gaagggttaa gtaagaagaa ctggtatctg cttatttgta accaccgtag
    32101 ttgggcagat attgtcgtac tgtgcgtgct gtttcgtaag cacattccga tgaataaata
    32161 tttcctcaag caacaactgg cctgggtgcc atttcttggc ctggcgtgct ggtcgctgga
    32221 tatgccgttt atgaagcgtt attcccgcgc ttatttatta cgtcatccgg agcgacgtgg
    32281 taaagatgtt gaaaccactc ggcgttcttg tgaaaagttt cgcctgcatc ccaccaccat
    32341 tgtcaatttc gttgaaggct cccgtttcac gcaagaaaaa catcagcaaa cccactccac
    32401 ttttcaaaac ttgttgccac caaaggcggc aggcattgcg atggcgctaa atgtactggg
    32461 taaacaattc gataaactgc ttaatgttac gctgtgttat ccggacaata accgtcagcc
    32521 gttcttcgat atgttaagcg gtaaactgac gcggattgtc gtacatgtgg atttacagcc
    32581 tattgccgat gagttacatg gcgactacat caatgacaaa agctttaagc gccattttca
    32641 gcaatggctg aactcattgt ggcaggaaaa agaccggctt ctgacgtctc tcatgtcatc
    32701 acagcgtcag aataagtaag ttttactcgc ctctgaatga gcagaggcga gtgagtattt
    32761 taatgaacaa aacgtccggc acgagacata aattcttctt cgctcatctc ctgtccgtta
    32821 aaaacaactt tacccggtgt ataacgcagc tgcagcgagg cggtattgtt gtcaatcgtg
    32881 attaactgaa acatctgacc taatgtcatc atcccgctga tttgtttatc agcctgcttt
    32941 tgcgcttttt ccgcatccat tccttcagat aaattaagct gttttgcggt ttctgtgacg
    33001 acattaagcg gtaacttcac atcaaaattg agcgatttga tatctttgtt tgtggatgat
    33061 gaagatttgg ctgggtcggc aatactgata tccagattgg catttagttc gccgagtgcg
    33121 ttcttccatc ttaccggttg tttaatggtc ggctcacttt tttgtaataa cggtaaatac
    33181 tctttgaaca atgcagcatt cacttcttgc agagcaactt catcgtttgc taactcaggg
    33241 tgtgcagcaa gctgcttctg catcgcaatg ttatactgga tgataaattg gcgcactgct
    33301 gagggatcaa tagattcagc aatcaatgac cactcaccac taccgagttg ctgattccct
    33361 tttgttaact tatcaagttc gtaggtgaga ttgacattga tataatcttt gtctttaacg
    33421 cgatccagcc tggtttttgc aacgaaagca tcgatttggg caacgtcttc cccgtgattg
    33481 ataatgttaa tctgattaaa tttcgactcg ctttcaccaa ccggaaattt cttttcttct
    33541 aatcgcgcca gagaatcaat ggtaaaactt tttgccgtta ctttgttcat tggtgataat
    33601 tgcagaacaa tatcttcgac tttgccttca atatccaggt tagacaatga actaccatca
    33661 ccattaaaag taaactggcc ctcaccaaaa gaaaactttc catactctgc aggggcaaca
    33721 tctaatgtcg tagagaatga gccaccaaac gcgaatcgca atgtcgcttc cacgggcgat
    33781 ttatttttcg cggcgataaa tagcggttgt gtcagttcat tattcactaa gttcactttt
    33841 gccgctgcca gtgctgggat gacattacca tgcattaaca ttatgatggg caacggaccg
    33901 tgctcaacat ccacatcaaa tacaactttc tggcctggct tgatattgag atcgggtgca
    33961 ccgttatcga atgtcatttg catttgaaaa cgggaattaa aaaagccttt gtcaaaattt
    34021 tgataactta acgtcatatc gtaggcatgt tcacctttct ttttagcatc gttaaaatct
    34081 ttaataaatt tttcgacacc aggctgaatt tgtgtacctg tgtaccatgt gccaccaccc
    34141 cagattacgg ctaacgcaac aataacacct gtagctgact tacgtatcat ataaatattc
    34201 cctgataaaa cagcaaaact ttgaatatcc acttatgctg aaataatgtc cagcggcagg
    34261 atgcattatc agaatgtatt tatatttatt aaataataaa aaa
//
LOCUS       DAZGSP010000049        33595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZGSP010000049 DAZGSP010000000
VERSION     DAZGSP010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.049"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            34..531
                     /gene="ftnA"
                     /locus_tag="ACHK74_002577"
     CDS             34..531
                     /gene="ftnA"
                     /locus_tag="ACHK74_002577"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFP0221374.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(569..808)
                     /gene="yecH"
                     /locus_tag="ACHK74_002578"
     CDS             complement(569..808)
                     /gene="yecH"
                     /locus_tag="ACHK74_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFP0221375.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            999..2210
                     /gene="tyrP"
                     /locus_tag="ACHK74_002579"
     CDS             999..2210
                     /gene="tyrP"
                     /locus_tag="ACHK74_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFP0221376.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2272..2937)
                     /gene="yecA"
                     /locus_tag="ACHK74_002580"
     CDS             complement(2272..2937)
                     /gene="yecA"
                     /locus_tag="ACHK74_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFP0221377.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3133..3219)
                     /locus_tag="ACHK74_002581"
     tRNA            complement(3133..3219)
                     /locus_tag="ACHK74_002581"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3183..3185),aa:Leu,seq:taa)
     gene            complement(3232..3305)
                     /locus_tag="ACHK74_002582"
     tRNA            complement(3232..3305)
                     /locus_tag="ACHK74_002582"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3271..3273),aa:Cys,seq:gca)
     gene            complement(3360..3435)
                     /locus_tag="ACHK74_002583"
     tRNA            complement(3360..3435)
                     /locus_tag="ACHK74_002583"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3400..3402),aa:Gly,seq:gcc)
     gene            complement(3587..4135)
                     /gene="pgsA"
                     /locus_tag="ACHK74_002584"
     CDS             complement(3587..4135)
                     /gene="pgsA"
                     /locus_tag="ACHK74_002584"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFP0221378.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4192..6024)
                     /gene="uvrC"
                     /locus_tag="ACHK74_002585"
     CDS             complement(4192..6024)
                     /gene="uvrC"
                     /locus_tag="ACHK74_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFP0221379.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGVDRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6021..6677)
                     /gene="uvrY"
                     /locus_tag="ACHK74_002586"
     CDS             complement(6021..6677)
                     /gene="uvrY"
                     /locus_tag="ACHK74_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFP0221380.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6819..6890
                     /locus_tag="ACHK74_002587"
                     /pseudo
     CDS             6819..6890
                     /locus_tag="ACHK74_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            6973..7149
                     /gene="yecU"
                     /locus_tag="ACHK74_002588"
     CDS             6973..7149
                     /gene="yecU"
                     /locus_tag="ACHK74_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFP0221381.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7136..7360
                     /gene="yecF"
                     /locus_tag="ACHK74_002589"
     CDS             7136..7360
                     /gene="yecF"
                     /locus_tag="ACHK74_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFP0221382.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7427..8149)
                     /gene="sdiA"
                     /locus_tag="ACHK74_002590"
     CDS             complement(7427..8149)
                     /gene="sdiA"
                     /locus_tag="ACHK74_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFP0221383.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(8379..9131)
                     /gene="tcyN"
                     /locus_tag="ACHK74_002591"
     CDS             complement(8379..9131)
                     /gene="tcyN"
                     /locus_tag="ACHK74_002591"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFP0221384.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(9128..9796)
                     /gene="tcyL"
                     /locus_tag="ACHK74_002592"
     CDS             complement(9128..9796)
                     /gene="tcyL"
                     /locus_tag="ACHK74_002592"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFP0221385.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(9811..10797)
                     /gene="dcyD"
                     /locus_tag="ACHK74_002593"
     CDS             complement(9811..10797)
                     /gene="dcyD"
                     /locus_tag="ACHK74_002593"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFP0221386.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(10902..11702)
                     /gene="tcyJ"
                     /locus_tag="ACHK74_002594"
     CDS             complement(10902..11702)
                     /gene="tcyJ"
                     /locus_tag="ACHK74_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0221387.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(11790..12341)
                     /gene="fliZ"
                     /locus_tag="ACHK74_002595"
     CDS             complement(11790..12341)
                     /gene="fliZ"
                     /locus_tag="ACHK74_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFP0221388.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(12387..13106)
                     /gene="fliA"
                     /locus_tag="ACHK74_002596"
     CDS             complement(12387..13106)
                     /gene="fliA"
                     /locus_tag="ACHK74_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFP0221389.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(13271..14764)
                     /locus_tag="ACHK74_002597"
     CDS             complement(13271..14764)
                     /locus_tag="ACHK74_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFP0221390.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRIRELSVQATNG
                     TNSDSDLTSIQSEIQQRLSEIDRVSGQTQFNGVKVLASDQDMTIQVGANDGETITIKL
                     QEINSDTLGLSGFGIKDPTKLKAATAETTYFGSTVKLADANTLDADITATVKGTTTPG
                     QRDGNIMSDANGKLYVKVAGSDKPAENGYYEVTVEDDPTSPDAGKLKLGALAGTQPQA
                     GNLKEVTTVKGKGAIDVQLGTDTATASITGAKLFKLEDANGKDTGSFALIGDDGKQYA
                     ANVDQKTGAVSVKTMSYTDADGVKHDNVKVELGGSDGKTEVVTATDGKTYSVSDLQGK
                     SLKTDSIAAISTQKTEDPLAAIDKALSQVDSLRSNLGAIQNRFDSAITNLGNTVNNLS
                     SARSRIEDADYATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            15019..16431
                     /gene="fliD"
                     /locus_tag="ACHK74_002598"
     CDS             15019..16431
                     /gene="fliD"
                     /locus_tag="ACHK74_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFP0221391.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPTIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISK
                     AINDADSGISASIVKVKDGNYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDGAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     IATEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            16446..16856
                     /gene="fliS"
                     /locus_tag="ACHK74_002599"
     CDS             16446..16856
                     /gene="fliS"
                     /locus_tag="ACHK74_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFP0221392.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            16856..17221
                     /gene="fliT"
                     /locus_tag="ACHK74_002600"
     CDS             16856..17221
                     /gene="fliT"
                     /locus_tag="ACHK74_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFP0221393.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNFS"
     gene            17299..18786
                     /gene="amyA"
                     /locus_tag="ACHK74_002601"
     CDS             17299..18786
                     /gene="amyA"
                     /locus_tag="ACHK74_002601"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP0221394.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSKFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTINLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(18820..19233)
                     /gene="yedD"
                     /locus_tag="ACHK74_002602"
     CDS             complement(18820..19233)
                     /gene="yedD"
                     /locus_tag="ACHK74_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFP0221395.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            19420..20625
                     /gene="yedE"
                     /locus_tag="ACHK74_002603"
     CDS             19420..20625
                     /gene="yedE"
                     /locus_tag="ACHK74_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFP0221396.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            20622..20855
                     /gene="yedF"
                     /locus_tag="ACHK74_002604"
     CDS             20622..20855
                     /gene="yedF"
                     /locus_tag="ACHK74_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFP0221397.1"
                     /translation="MKNIIPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            20964..21632
                     /gene="yedK"
                     /locus_tag="ACHK74_002605"
     CDS             20964..21632
                     /gene="yedK"
                     /locus_tag="ACHK74_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFP0221398.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            complement(22025..22339)
                     /gene="fliE"
                     /locus_tag="ACHK74_002606"
     CDS             complement(22025..22339)
                     /gene="fliE"
                     /locus_tag="ACHK74_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFP0221399.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            22554..24212
                     /gene="fliF"
                     /locus_tag="ACHK74_002607"
     CDS             22554..24212
                     /gene="fliF"
                     /locus_tag="ACHK74_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFP0221400.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIATIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            24205..25200
                     /gene="fliG"
                     /locus_tag="ACHK74_002608"
     CDS             24205..25200
                     /gene="fliG"
                     /locus_tag="ACHK74_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFP0221401.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            25193..25879
                     /gene="fliH"
                     /locus_tag="ACHK74_002609"
     CDS             25193..25879
                     /gene="fliH"
                     /locus_tag="ACHK74_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFP0221402.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            25879..27252
                     /gene="fliI"
                     /locus_tag="ACHK74_002610"
     CDS             25879..27252
                     /gene="fliI"
                     /locus_tag="ACHK74_002610"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFP0221403.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            27271..27714
                     /gene="fliJ"
                     /locus_tag="ACHK74_002611"
     CDS             27271..27714
                     /gene="fliJ"
                     /locus_tag="ACHK74_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFP0221404.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            27711..28838
                     /gene="fliK"
                     /locus_tag="ACHK74_002612"
     CDS             27711..28838
                     /gene="fliK"
                     /locus_tag="ACHK74_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFP0221405.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            28943..29407
                     /gene="fliL"
                     /locus_tag="ACHK74_002613"
     CDS             28943..29407
                     /gene="fliL"
                     /locus_tag="ACHK74_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFP0221406.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            29412..30416
                     /gene="fliM"
                     /locus_tag="ACHK74_002614"
     CDS             29412..30416
                     /gene="fliM"
                     /locus_tag="ACHK74_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFP0221407.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            30413..30826
                     /gene="fliN"
                     /locus_tag="ACHK74_002615"
     CDS             30413..30826
                     /gene="fliN"
                     /locus_tag="ACHK74_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFP0221408.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            30829..31194
                     /gene="fliO"
                     /locus_tag="ACHK74_002616"
     CDS             30829..31194
                     /gene="fliO"
                     /locus_tag="ACHK74_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFP0221409.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     VPKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            31194..31931
                     /gene="fliP"
                     /locus_tag="ACHK74_002617"
     CDS             31194..31931
                     /gene="fliP"
                     /locus_tag="ACHK74_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFP0221410.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            31941..32210
                     /gene="fliQ"
                     /locus_tag="ACHK74_002618"
     CDS             31941..32210
                     /gene="fliQ"
                     /locus_tag="ACHK74_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFP0221411.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            32219..33004
                     /gene="fliR"
                     /locus_tag="ACHK74_002619"
     CDS             32219..33004
                     /gene="fliR"
                     /locus_tag="ACHK74_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFP0221412.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            33294..>33595
                     /gene="rcsA"
                     /locus_tag="ACHK74_002620"
     CDS             33294..>33595
                     /gene="rcsA"
                     /locus_tag="ACHK74_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFP0221413.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEVCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKN"
BASE COUNT         8272 a         8599 c         8433 g         8291 t
ORIGIN      
        1 cagcaacaaa tataaccttt gtggagcact atcatgctga aaccagaaat gattgaaaaa
       61 cttaatgagc agatgaacct ggaactgtac tcttcactgc tttatcagca aatgagcgcc
      121 tggtgcagct atcatacctt cgaaggtgct gccgcgttcc tgcgccgtca cgcccaggaa
      181 gagatgacgc atatgcagcg tctgtttgat tacctgactg ataccggcaa tttaccgcgt
      241 attaataccg ttgaatctcc gtttgctgaa tattcctcac ttgatgaatt attccaggaa
      301 acctataaac acgaacaatt aatcacgcag aaaattaacg aactggctca tgctgcaatg
      361 accaatcagg actacccaac atttaatttc ctgcaatggt atgtttctga gcagcatgaa
      421 gaagagaaac tgttcaaatc gattattgat aaattaagcc tggcaggcaa aagcggcgaa
      481 ggtctgtatt ttatcgacaa agaactctct accctcgaca cacaaaacta atgctctcag
      541 gcggcaggtt ttctgccgcc gtttcaaatc agtgacggca aatcttactc tgatcggtcg
      601 aaaaaccttc ttctgaaggt ataaatttgc cttttgctgc cagaaacgct accagctctc
      661 ccgcagtcat cccttctgcc gagcaggtgt gaaaacgtgc ctgttcacca aaacgcgctt
      721 taattgctgc ttccagactg gcatgcgtat attgctcgcc tgattcaatc atcatattta
      781 acacttcatg accgtgaata gagtccatcg tccctcctca aaaaaagcct agcgtagcga
      841 ttgccgctta tgaagacttt gcgccagcgc aggactgaat gctttttatt gtacatttat
      901 atttacacca tatgtaacgt cggtttgacg aagcagccgt tatgccttaa cctgcgccgc
      961 agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga accctgggaa
     1021 gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg ccgctggctg
     1081 cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg gcgttgatgt
     1141 gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat accggtcttg
     1201 gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc ttcagtatga
     1261 tgttcttaat gtatgctctg actgcggcat acatcagcgg tgccggtgaa ttgttggcct
     1321 ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg ctgttgttca
     1381 cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta tttaaccgtt
     1441 ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg ctaccgcata
     1501 ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggctctg tcagcaatcc
     1561 cggtgatttt tacctcattt ggttttcacg gtagcgtgcc gagtattgtc agctatatgg
     1621 atggcaacgt tcgtaagcta cgctgggtgt ttataaccgg tagtgcgatc cccctggtgg
     1681 catatatttt ctggcaggtg gcaacgcttg gcagcattga ttcaacaacc tttatgggat
     1741 tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa atggtggcct
     1801 ctccgcatgt tgagctggca gtgcatttat ttgctgattt agccctcgcc acgtcatttc
     1861 tcggcgttgc gttaggctta tttgattatc tggctgattt atttcagcgt tcaaataccg
     1921 ttggtggacg gttgcaaact ggtgcaatta cctttctgcc gccgttggcg tttgcactgt
     1981 tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg gcggtactgg
     2041 cattgattat cccttcgctg ttgacctggc aaagcagaaa gcacaatcct caggcgggtt
     2101 accgggtgaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt attgctgtga
     2161 ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga tcagatagcc
     2221 tcaaattcct tattgggtgc cagaattaac gctgacaccc aatttgacct cttaatgcag
     2281 gcagcactgc ttaaatttct taccactacc gcacgggcaa ggatcgttac gccccggttt
     2341 ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca tccagtacgc
     2401 atgtagatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg cttctggcga
     2461 cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg cctccagcgc
     2521 tggttttaac gaatcgggca acgttgacca gtcagaaagt gccacgcccc gcatatagcc
     2581 aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca agccgaataa
     2641 cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca tatgttgaaa
     2701 agccagattc ataaagcgcg tcatctcttt ctctgacgcc cagcgcggca catagtcagc
     2761 cccaccccac acggcaacca gccactgttc cggttcaatc tcttgcggag aactcaacac
     2821 cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag tgttgtattt
     2881 ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg ttttcatacg
     2941 cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc gtatcagaga
     3001 tactttttga gtggctttgc tggtgattaa aaattaggga gggtgtaacg acaagttgca
     3061 ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattgcttt gattttattc
     3121 ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa cgccgaggga
     3181 ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa gtggaggcgc
     3241 gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg ctttgttaac
     3301 gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt cttttaaact
     3361 ggagcgggaa acgagactcg aactcgcgac cccgaccttg gcaaggtcgt gctctaccaa
     3421 ctgagctatt cccgcattca tcaagcaatc agttaatcac ttgattttat tatcgtctgg
     3481 caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg agtcaacgat
     3541 atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac tgatcaagca
     3601 aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt accgcagcca
     3661 caaagaaaag tgcaataccg gcgtactcaa cccaaatgtt cggacgccac agcagccagg
     3721 ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagaca gccacgctac
     3781 tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt tcacgggcga
     3841 tcatcgttgc cgccggtagg gtcacccacc agctgtgata atgctcggtt accagcacca
     3901 tggcgatagc cacgagaact ttatctgcta cagggtcaag gaaagcacca aaccgggtac
     3961 tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc gcgacgcaga
     4021 aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc aatacaaaga
     4081 atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat tgcataatga
     4141 cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac atcaatgttt
     4201 caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt ttgcaatttc
     4261 ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca acatttgccg
     4321 acgttttggc ccgacgcctt caatggtttc cagggagctg gtatttttga ccttcgcccg
     4381 ttttttacgg tgcccgccaa tcgcgtgatc gtgtgattca tcgcgaatat gctggataac
     4441 atgcagcgcg ggagaatctg gcggcaaact gaatccctca ccttccggct caaagaacag
     4501 tgtttccagc ccagccttac gatctactcc tttggcaacg ccaagtagca gtggatgatt
     4561 tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa gctggccttt
     4621 gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt taccataacg
     4681 ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc ctgtaatgtt
     4741 atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac aggaagcgac
     4801 ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca cttccggcaa
     4861 tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct gcgaaagttt
     4921 gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg ccctatcgcc
     4981 gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg aatcggcgag
     5041 cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg tgcgcatctg
     5101 gctgccttgt aaatagaact ggcctacgaa ggtttctacc acctcgctca gttccgtacc
     5161 gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac gaatgaacaa
     5221 tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcga ggtcgtcgcc
     5281 ggtattggaa acgaattgtt tttcggtgac gcgtcgcacc gcctgaattt ggtcgcgaat
     5341 acgtgcagct tcttcaaact ccagattctg gctggcagtt tccatacgac taatgagttg
     5401 cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga cctgctgagc
     5461 gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc ctatctggta
     5521 ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc gaatggggaa
     5581 aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga acgggccgaa
     5641 atattcacct ttggcatgct tcgcaccacg atgcatcgcc agacgcgggt gggtatcacc
     5701 actcaggaag ataaaaggat atgatttatc atcgcgtagc aaaacgttgt aacgcggctg
     5761 atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtgt gagtaaccgt
     5821 tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag cgaggttgct
     5881 acggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat agataaccgt
     5941 accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt ttaaaaacgc
     6001 ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca cagaccatgg
     6061 cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact gaacatacga
     6121 tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat ctcattgacc
     6181 ttctggccct tggtgatcat cagcataatc tgcaattcac gttcagacaa actggcaaat
     6241 gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc gatgtcagaa
     6301 gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc ctgcggagcc
     6361 gcgcctttgc tgaggtagcc cgcagcaccg gcctgcatga ctttcgctgg taaagggttt
     6421 tctgtatgga cggtaagcat gatgattttg acatcagctg tggaacgcgc gattttacgc
     6481 gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac gtcaacggca
     6541 tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctcaccgac gacttttata
     6601 ccctttatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg gtcatcaaca
     6661 agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact gatagttagt
     6721 cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt gggtattcga
     6781 tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc gtcaccaacg
     6841 gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat tgactttatt
     6901 cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga acaatacggg
     6961 caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgtatt ttatagcact
     7021 gccgatatca cgctcaaaaa acaaccactg ctatttttag caaagcttat ggtatactcc
     7081 gccgccttaa catttttcac cgcaaatttt cattgcaaca tgacgaggaa aataaatgag
     7141 tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag tctcctgcct
     7201 gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg gccgcgtgat
     7261 gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg cagtattttc
     7321 caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg gctgagagcg
     7381 tgcctttcag ccggtttttg catctggcac gcagaaagaa aagcgatcaa attaagccag
     7441 tagcggccgc gtaacaggcc acctgggtct tatttggtgc attgattttt ttctgcatgt
     7501 ttttctgatg gaaattgacc gtattctcag agattgacaa aatcatcgct atctctgctg
     7561 atgtcttccc ttccgccgtc catttcagaa tttctttttc gcgcttgctg aaattcatct
     7621 ctggcgtcat cactatttca tcatttaaac gcatcagagc catcagactt tcgcgcacca
     7681 gtaactgcat ttttaattgc agttcatcac taagaatggg tatttcgcgc gtgctgcaac
     7741 gggaaaagga caaaaagccc agcgcccgat ttggcagcat taaatactga gtgacaccgc
     7801 ggcgtaaacc atgtgcgtgc gcggcttccc ataatggctg tgcttcgctg aataagtcat
     7861 cattccacat taaatggccc tgactaaagt tttcagggtt cagcacagga tcaattgcga
     7921 gaaaattttt tgcctgataa taactaaccc acgactcagg gtaattggtg tagaaagcca
     7981 ctttaggtcg cgtgaatggt accgggtggc gaacacataa cgaatagtaa tcgtactcca
     8041 gctgctgagc ctgaagctct atttcatggt agacctcttc tgcggcctcc atcctctgaa
     8101 aacgcaacag catcgtgcga cgccagctga aaaaatccgt atcctgcata gtaaaccgca
     8161 acgcccctga gagtgagtat catttataat gataattcaa actatcacat aaataagatt
     8221 tatatataat ttatattatt caggcaatga attacttttg caagccatcg cattctctta
     8281 tgttattaat gagttatgct gatttgttaa gcagttttat cacgcttgaa atggcgtccc
     8341 gccccgacag gtgaatcgtc ggggctgatt ttttcttatt attgcagcag aaacttctcg
     8401 aggaactggc gggtgcgagg ctgctgggga tcagcaaata acgcttttgc tgccccctgc
     8461 tcgactatcc gcccctggtc cataaaaatc gcccggtccg caacatcccg ggcaaagctc
     8521 atttcgtgcg tcacaatcac catcgtgcgc ttttcctgcg ccagctggcg gattgtgttc
     8581 aggacttcgc ccaccagctc tggatccagc gctgacgttg gctcgtcaaa caaaatcacc
     8641 tcaggacgca ttgccagcgc acgcgcaatc gcaacacgct gctgttgacc gccagacaaa
     8701 cgacgtggat agctggtttc tttaccggcc agcccaactt tcgccagcag ctcgcgagcg
     8761 cgcgccgtgg cctcttcttt cggttcgcct ttgacgatca ccggcccttc aataatgttt
     8821 tccagcaccg tacgatgcgg aaacaaatta aagttctgga agacaaaccc gacgtgctga
     8881 cgcaactggc gaatcagcga tttttgctga cttaatgaac gtgcagtatc aatagtgata
     8941 tcgccaacgg tgatcgttcc cgcttccggt tgttccagca gatttatgct gcgtagcaac
     9001 gtggttttgc cggaaccact cggaccgata attgccacca cttcgccagg ctttacctcg
     9061 aggtcgatac cgtgcagcac cgtctgaccg tgaaattttt tcaccaggtt cttaacttca
     9121 atggcactca ttttggttct ctctcctggc gattaagttg attctcaaaa tggttctgca
     9181 acgtcgataa tactgtcgcc atgatccagt agatgagcga agccgccagg tacatggtga
     9241 acacctccag cgtgcgcgag gtaatcaact gcgcctgacg gaacagctcc ggcacctgga
     9301 ttgtcgcggc cagcgacgta tcctttacca ggctgataaa gctgttcgac agcggcggca
     9361 acgccacccg cgccgcctgt ggcaaaatcg cgcgacgcat agtctgccat ggcgtcatac
     9421 caatactggc agctgcttcc cactgacctt tatcaataga agaaatggcg gcgcgcagtg
     9481 tttcggcggc ataggcggca gtgttcagcg acagaccaat cattgctgac ggaatgggat
     9541 ctaattcgat accaaactgc ggcaggccgt aatagatcat aaacagttgt gcgatgagcg
     9601 gtgtaccacg aaaaatagag atataaaaac gcgccagcca gcgcacgggc cagattggcg
     9661 acaggcgcat cagcgcgaga ataaacccga gcagtaagcc aaaaaacatg ccgccgatac
     9721 tgagttgcag cgtataccca gcccctttca acaggaacgg caaggaatca ataaccagtt
     9781 gtatactttc ttgcatgagc gttcccggag ctaaacgtgg ggatgatagg cgaacagcgc
     9841 aggcgcaccg ccggtatgaa taaacagaat cggcccttca tctttgaagc gtttctgact
     9901 gataccgcca atcagccccg ccatcgcttt tccggtatac acaggatcaa gcagaatgcc
     9961 ttccagccgc gccagcaatt tcactgcttc catgccttcg tcgttcggca cgccgtagcc
    10021 aggtgcaaaa tagtcatccc agagtaaaat ttccgctgat gcggtcagct ccagttcttt
    10081 cgcaatcgcc tgttgtaggt taaccacttt cggcaactga tcggcaacgg aacgcgacac
    10141 ggtcacgcca atcagttcgc tttcaggcat caggtgttcc agcccaacag ccagtccggc
    10201 gtgagttccg gcactgcccg atgcgactac caccgacgaa atattaaccg ccccttcaca
    10261 ctgttgcgcg atttccagcg cactctccac ataacctagc gcgccaagag cattagaacc
    10321 gccaaccgga atgacatacg ggcgaaagcc ttgtgcttcg actcgcgtcg ccagctcttc
    10381 cagttgggca ttgggatcgg tcagtgcatc gcacatttca atctgggtat tgaacagatc
    10441 cagcaacaaa cgattgccgt tggttaaata gttttctgcg gttgtgccaa taggattttc
    10501 cagcagcgcc acgcagtgca gaccgagttt cgccgcgact gcggcagtct ggcgcacatg
    10561 gttagactgg atcgcgccgg cagtaatcag cgtatcggca ccttcgcgca gggcatctgc
    10621 cgcgagaaat tccagcttac gtaatttatt gccgcccatt gccatgggtg tgacgtcatc
    10681 ccgtttgatg aaaatttccc gtccaagata atcagaaaag cgcggcagat attcgagcgg
    10741 cgttggcgcg ccgataaact ccaaccgtgg aaaacgggtt aaattatgca gtggcataac
    10801 agcctccgat gtgtgttgtt gtgattttct tattatgcac gctgaaaacg cgtaaataaa
    10861 aaaggcgcta gtgaaagcgc ccttttttgt cattatgctg attatttggt cacatcagca
    10921 ccaaaccatt tttcggaaag agcttgcaga gtgccgtctt tttgcatttc cgcaattgca
    10981 tcattcaccg ctttcagcag gtcttcattt cctttacgca gcgccacgcc agactcctga
    11041 cgggagaatg cttcaccggt tactgccagc gtatcgttgg ttttcttcac cagatccagc
    11101 gccgccagac gatcaacgag gatcgcatcg atacggccta cgcgcagatc ctgatatttg
    11161 gtcgggtcat catcataggt acgcacatcg acgccctgaa cattctgccg cagccactct
    11221 tcatagttgg tgcccagacc gacacccact tttttgcctt tcagatcagc agccgtttta
    11281 atggtgcctt cgttaccttt tttcaccagc gcctgaatac cggaaatggt gtaaggggta
    11341 gagaaatcgt attttttctt gcgctcgtca gaaatggtga cctgattaat caccacatca
    11401 atgcgtttag agtccagcga cgccagcata ccgtcccatt tggtcggttt tagtgacgcc
    11461 tcaacgccaa gatgttttgc cagctgttgg gcaaattcca cttcaaaacc ggttaatttg
    11521 ccgtcatccc cctgaaaact gaacggcgga taagttcctt ccagccctac cagcagcgtg
    11581 ccgcgctctt tgactttatt aagcagacct tcatctgcaa aacttttaac gctcatgccc
    11641 gcaaccagcg ccacggccat cacacccatc aatgcctgac gtcccagatg tgctaatttc
    11701 atattcaccc cgaatgttgt tatgtctgtt tgcagtgtag agccattgtt tgtaaacaca
    11761 aaaacaactc cgctacatct tattcttatt taatatatat cagaagcagg caggctggag
    11821 gatttctgca caagtccggt acacgtttga cgctgatagt gttgatactt ccgtaatgcg
    11881 atgcggtaat tgttggtgct ggtggtgggc agccacgggg cgaggatttc atcaaggaaa
    11941 ccgtcctgca gctgatcgag ggaaatattt tgctcgtgta gatgattccc caaacggcgc
    12001 agacgaacga catattcacg caccgtacct gggctcattt cagtttgctc aaaaagaaat
    12061 tgcttaaagc cgataatatc gaaaaaatca ctctgcgttt tgcaatgtaa atcaccgcaa
    12121 aatcggcaca atgccgccca tgattgttgt tccacttgcc agccattttc atcaagcagc
    12181 gtgtccaggc gggaaatctc gattttattc actattttgc cgtcgcgaac taaggtaatg
    12241 cgatcgagta atttacggca atgcgcgcaa tgggtctggc tgtgtttaaa gtctttaaga
    12301 tagcggctta atggccgtct tttcaggtgc tgcaccatca ttaagaactc ctggtagtca
    12361 aaattaaagt gcggcattta ctgacgttat aacttaccca gtttagtgcg taaccgttta
    12421 atggcctggc tgtgtaactg actgacccgc gattccccga cctccagcac cgcgccaatc
    12481 tctttgagat tcagctcttc ctggtaatag agggttaata ccagtttttc gcgctccggc
    12541 aacgtttcga tagcttccat cactcgctgg cgcagattac tgtccagcag ttgttgtagc
    12601 gggttttctc gctgatgatc atcagtaacc agttcgatgc tatcgccgtg ctcttcgcgc
    12661 cactcatcgt aggagaagag ctggctgtta ttggtgtcga gcaacatttg gcgataatcg
    12721 gcaatatcga tccctaaacg ttccgctacc tcagtttccg tggcgttgcg gccaagttcc
    12781 tgctccagtt gccctattgc ctgtgccact tcacgcgcgt tgcgtcgcac gctgcgcggc
    12841 acccagtcac ggctgcgaag ttcatccagc atagcgccac ggatacgctg cactgcgtaa
    12901 gttgtaaatg ccgttccttg tagggcgtca tagcgttcga cggcattaag taacccaatg
    12961 ccgcccgcct gtagcagatc gtcaagttcc acgctcgcgg gcagtcgaac ctgcaggcgc
    13021 aatgcttcgt gacgcaccag cgggacataa cgctgccaca gcgagtgttt atccattaca
    13081 ccttcagcgg tatagagtga attcacgata aacagccctg cgttatatga gttatcggca
    13141 tgattatccg tttctgcagg gtttttaatc ggacgattag tgggtgaaat gaggggttat
    13201 ttgggggtta ccggtaaatt ccatgcagaa aaaaccccgc cggtggcggg gtttgagcga
    13261 taagtgtaaa ttaacgcagc agagacagta cgttctgcgt ggtctggtta gcctgtgcca
    13321 gaacagaagt acctgcttgt tgcaggatct gcgcacgaga catgttagac acttcggtcg
    13381 cgtagtcagc atcttcgata cggctacggg cagaagacag gttgtttacg gtgttgccaa
    13441 ggttggtgat ggcagagtcg aaacgatttt gaattgcacc taggttagaa cgcaacgagt
    13501 caacctgaga cagtgcttta tcgatagcag ccaaaggatc ttctgttttc tgcgtagaaa
    13561 ttgctgcaat agaatcagtt ttcaggctct taccttgtaa atcactaaca ctgtaagttt
    13621 tgccatcggt tgcagttaca acttcggttt tgccatcgct tccacccagt tcaactttaa
    13681 cattgtcgtg tttgacaccg tcagcatcag tgtaagacat tgttttaacg gaaactgctc
    13741 ctgttttctg atcaacattc gctgcatact gtttaccgtc atcaccaatc aacgcaaatg
    13801 aaccagtatc tttgccattg gcgtcttcta acttaaagag ttttgcacct gtgatagaag
    13861 cggttgcggt atcagtaccc aactgaacat caatagcccc cttccctttc accgttgtga
    13921 cttcctttaa attaccagct tgaggctggg tacccgctag agcccccagc ttcagcttac
    13981 ctgcatcagg agatgtcgga tcatcctcca cagtaacttc ataataacca ttttcagcgg
    14041 gtttatctga accggcaact ttaacgtaca acttaccgtt agcatcagac ataatattac
    14101 cgtcacgttg gcccggagtc gtagtgcctt taactgtagc tgtaatatct gcatcaagtg
    14161 tattagcgtc agcaagctta actgtcgatc caaaataggt tgtttcagcc gttgcggctt
    14221 ttaatttagt aggatcttta ataccaaaac cagataatcc cagtgtgtcg gaattaattt
    14281 cctgcagttt aatagtaatt gtttcgccgt cgtttgcacc aacctgaata gtcatatcct
    14341 gatcagaagc cagcacttta acgccgttaa actgagtctg accagaaaca cggtcaattt
    14401 cactcagacg ctgctggatt tcggactgga tggaggtcag gtcactgtca gagttagtac
    14461 cgttagttgc ctgaacagaa agttcacgaa tacgctgcag gttgttgtta atttcattca
    14521 gcgcaccttc agtggtctgc gcaacagaaa taccatcatt cgcgttacgg gaagcctgag
    14581 tcagaccttt aatatttgct gtaaaacggt tagcaatcgc ctgacctgct gcgtcatctt
    14641 tagcactgtt aatacgcagg ccagaagaga gacgttcaat ggcggagctc agagaagact
    14701 gagatttgtt caggttattc tgggtcaaca gcgacaggct gtttgtatta atgacttgtg
    14761 ccatgatctt ttccttatca attacaactt gatgttattg ggctgttgcc cacggtttct
    14821 caccgtaccc cttgtatcgg cacctgaatt tcgaacttta gaaaatttta gtttctctcc
    14881 tgttttttct taacggctga aatatccgta atatataact ctactctgcg catcatcttc
    14941 gtgaatcaac tattaaactt tgcgcacaaa ttgccgataa cccgcttatc tactgtttgc
    15001 aatcaaaagg aattaggtat ggcttcattt acatctcttg gcgttggttc gaatctgccg
    15061 ctggatacat tgctaaacaa cctgaccatc gctgaaaaaa aacgcttaaa cccaattact
    15121 cagcaacaga gtgacaatac cgcacgtctg acagcctatg gcacattaaa aagtgcatta
    15181 gaaaaattcc agactgcaaa tactgcgtta aataaagccg atctgttcaa aagcactacc
    15241 gttaccagta gcaatgaaga ccttaaagtt tccaccgaag caggtgctgc tcccggtatc
    15301 tataccatca gcgtaaccca actggcgcag gcgcaatctt tacgaactga ttccccgaca
    15361 atcattgcat caacaaaaga tgcactgggt gatgagtcat cagatacacg cacaataaaa
    15421 attacccaag acggtcgtaa agagccgctg gaaatcaagc tcaataaaga tcagacgtca
    15481 ttggatgaga tcagcaaagc cattaacgat gccgatagtg gaatttccgc tagtatcgtt
    15541 aaagtcaaag atggtaacta tcaattggta ttgaccgcca gtgaaggtct ggccaataaa
    15601 atgaccatat ctgttgaggg tgacagtaaa ttaaatgatc tgctggctta tgacagcaag
    15661 accaacaccg ggaatatgaa ggaactggtt aacgcgcaaa atgcacagct aaacgttaac
    15721 ggtatcgata ttgaacgttc aagtaacaaa attaccgatg ctcctcaggg agtgactctc
    15781 gatttgacca aaaaagtcac tgacgtccgg gtcacggtga caaaaagcaa cgataaagca
    15841 accgaagcaa ttaaaggctg ggtggattca tataactccc tcattgacac ctttaacacc
    15901 ctgacaaaat ataaagaagt agacccaggt gctgaagcac aagataaaaa taatggtgca
    15961 cttctcggag acagcgttgt acgtacaatt caaagcggca tccgcgcaca atttgcaaac
    16021 ggtgccagcg acggtgcctt caaaacatta aacgagatcg ggattaagca ggacggcacg
    16081 acgggtaagc tgaaaattga tgatgataaa ctcaaaaaag tcctgaatga aaatacagca
    16141 tccgtacgtg aattgctggt aggcgatggt aaagaaacag gaattaccac caaaattgcc
    16201 accgaggtaa aaggttacct tgccgatgat ggcattattg atagcgcaca ggacagtatt
    16261 aacgccacac tgaaaaaact gaccaaacag tatctatccg tcagtgccag cattgatgac
    16321 accgtggcgc gttacactgc acagtttacg caattagata ccatgatgag caaactgaat
    16381 aacaccagta cctatctcag ccaacaattt actgcaatga gtaattcctg atcacggagg
    16441 tcagcatgta taccgcgagc ggctcaaaag cctatgcaca aattggcgtc gaaagcgccg
    16501 taatgagcgc cagccagcag cagctggtca ccatgctatt tgatggagtg ctgagcgcac
    16561 tggttagagc gcgcctgttt atgcaggaca acaatcagca aggcaaaggc gtctctttgt
    16621 caaaagcgat caacatcatt gagaacggac ttcgggtgag tcttgatgaa gagagcaaag
    16681 acgaattaac ccaaaacttg attgctcttt atagctatat ggtcaggcgc ttgctgcaag
    16741 ccaatttacg caacgatgtc tccgcagtcg aagaagtgga agcattaatg cgtaatattg
    16801 ccgatgcctg gaaagagtcg ttactctccc cttctttgat tcaggaccca gtctgatgaa
    16861 ccatgcaccg catttgtatt tcgcctggca acaactcgtc gaaaaaagcc agctcatgtt
    16921 acgcctggca acggaagaac aatgggacga actcatcgcc agcgaaatgg cgtatgtgaa
    16981 tgcggtgcag gagatagcac acctgactga agaggttgcc ccgtccacca cgatgcagga
    17041 gcagctccgc ccgatgctgc gcctgattct cgacaacgaa agcaaggtaa agcagttatt
    17101 acagattcgt atggatgaac tggcgaaact ggtcggtcag tcatcggtgc aaaaatcggt
    17161 gttaagtgcc tatggcgatc agggcggctt tgtgctggct ccgcaagata acttctcgtg
    17221 aatcttaacg agctaatggt tcgcgaataa tccgattacg gctacgcttc taatgttccc
    17281 cttgaatgga gtcgaagaat gcgtaatccc acgctgttac aatgttttca ctggtattac
    17341 ccggaaggcg gtaagctctg gcctgaactg gccgagcgcg ccgacggttt taatgatatt
    17401 ggtatcaata tggtctggtt gccgcccgcc tataaaggcg catcgggcgg gtattcggtc
    17461 ggctacgact cctatgattt atttgattta ggcgagtttg atcagaaagg cagcatccct
    17521 actaaatatg gcgataaagc acaactgctg gccgccattg acgccctgaa acgtaatgac
    17581 attgcggtgc tgttggacgt ggtagtcaac cacaaaatgg gcgcggatga aaaagaagct
    17641 attcgcgtgc agcgtgtaaa tgctgatgac cgtacgcaaa ttgacgaaga aatcattgag
    17701 tgtgaaggct ggacgcgtta caccttcccc gcgcgtgccg ggcaatactc gaagtttatc
    17761 tgggatttca aatgttttag cggtatcgac catatcgaaa accctgatga agatggcata
    17821 tttaaaattg ttaacgacta caccggcgaa ggctggaacg atcaggttga tgatgaatta
    17881 ggtaatttcg attatctgat gggcgagaat atcgattttc gcaatcatgc cgtgacggaa
    17941 gagattaaat actgggcgcg ctgggtgatg gaacaaacgc aatgcgacgg ttttcgtctt
    18001 gatgcggtca aacatattcc ggcgtggttt tataaagagt ggatcgaaca cgtacaggaa
    18061 gttgcgccaa agccgctgtt tattgtggcg gagtactggt cgcatgaagt cgataaactg
    18121 caaacgtata ttgatcaggt ggaaggcaaa accatgctgt tcgatgcgcc gctgcagatg
    18181 aaattccatg aagcatcgcg catggggcgt gactacgaca tgacgcagat tttcacgggt
    18241 acattagtgg aagccgatcc tttccacgcc gtgacgctcg ttgccaatca cgacacccaa
    18301 ccgttgcaag ccctcgaagc gccggtcgaa ccgtggttta aaccgctggc atatgcctta
    18361 attttgttgc gggaaaatgg cgttccttcg gtattctatc cggacctcta cggtgcgcat
    18421 tacgaagatg tcggcggtga cgggcaaacc tatccgatag atatgccaat aatcgaacag
    18481 cttgatgagt taattctcgc ccgtcagcgt ttcgcccacg gtgtacagac gttatttttc
    18541 gaccatccga actgcattgc ctttagccgc agtggcactg acgaatatcc cggctgcgtg
    18601 gtggtcatgt cgaacgggga tgatggcgaa aaaaccatta atctgggaga gaattacggc
    18661 aataaaacct ggcgtgattt tctggggaac cggcaagaga gcgtagtaac cgacgaaaac
    18721 ggtgaagcga ccttcttttg caacggcggc agcgtcagcg tgtgggttat cgaagaggtg
    18781 atttaaattc atccccggcg gcaagccggg gagatttcat tacggcagtg gcgtcggtaa
    18841 cggcgcttta tccagcgcgg cggcacactc tgccgtcggg cgatccacac gctccatcgt
    18901 cataccatcg tactcaatgg tgttaccgtc acgctcaacc tcgtacagct cacgttttac
    18961 ggtaacgtta gtgagatcat cagacatcaa tgtcaacttg cccggcacgg caatcacccg
    19021 ctgccactga cggcaatcca gggtatcgcc ctctttagtg acaatcaggc tgcctatcgc
    19081 ttccggactg accagcgcac gttgcggccc tttggtctgc cagtagcccg ccagccagtc
    19141 cggcgcgggg gttttcacga cattgttata gttttcgact tcggcacagc ccgccagcaa
    19201 catgagcgcg cctgcaattg cgagtttttt catcattcgt cctgcatgag aagaaaagag
    19261 gattgtggca ttaaagccct gatgtcgcca gcctttccag gttcagagag aaatttgatc
    19321 taactattat ttttgtgaat tattaccccg aaaataaatt tgcatgatgt atgattcgcg
    19381 attcttgttt caaaccttct gagttcagag gctacattca tgtcatggca gcaattcaaa
    19441 cacgcctggt tgattaaatt ctgggcaccc atccctgcgg tcatcgcggc gggtattctc
    19501 tccacttact attttggcat tactggcacc ttttgggctg tcacgggtga atttacccgt
    19561 tggggcggtc agctcctgca gctgtttggc gtccatgctg aagagtgggg ttattttaaa
    19621 attatccatc tggaaggatc gccattaacc cgcatcgacg ggatgatgat cctcggcatg
    19681 tttggcggct gctttgccgc agcgctgtgg gccaacaatg tcaaactgcg catgccacgc
    19741 agccgtatcc gcattatgca ggccattatt ggcggtatta tcgctggttt tggcgcgcgt
    19801 ctggcaatgg gctgtaacct ggcggcgttc tttaccggta ttcctcagtt ctcgctgcat
    19861 gcctggttct ttgccatcgc cactgccatt ggttcatggt ttggcgcacg ctttaccctg
    19921 ctgcccatct tccgtattcc cgtgaaaatg cagaaagttt ctgccgcctc accgctgacg
    19981 caaaaaccgg atcaggcgcg gcgtcgtttt cgtctcggga tgctggtctt tttcggtatg
    20041 ctgggctggg cgctgctcac ggcgatgaac caaccaaaac tggggctggc aatgctgttt
    20101 ggcgtcggct ttggtttact gattgaacgt gcgcaaatct gctttacttc ggcgttccgc
    20161 gatatgtgga tcaccggacg tacccatatg gcgaaagcaa tcattatcgg tatggcggtg
    20221 agtgccatcg ggatcttcag ttacgtacag ttaggcgttg aacccaaaat catgtgggcg
    20281 ggaccaaacg cggtaattgg tggtttactg tttggttttg gcatcgtgct ggcaggcggc
    20341 tgcgaaaccg gttggatgta ccgcgcggta gaaggtcagg tgcactactg gtgggtcggt
    20401 ctgggcaatg tgatcggctc aaccattctg gcgtattact gggatgattt cgctccggcg
    20461 ctggccaccg actgggacaa aatcaacctg ctgaaaacct ttggcccgat gggtggcctg
    20521 ctggtgacat atttgctgtt gtttactgcg ctgatgttga ttatcggctg ggaaaaacgc
    20581 ttcttccgcc gtgcggcacc gcagactgct aaggagatcg catgaaaaat atcattcctg
    20641 attaccgtct tgatatggtg ggtgaaccct gcccttatcc ggcagtcgca acccttgagg
    20701 cgatgccgca gttaaaaaaa ggtgagatcc tggaagtggt gagcgactgt ccgcagtcga
    20761 tcaataatat tccactggat gctcgtaatc acggctatac ggtgctggat attcagcaag
    20821 acgggccgac cattcgttat ttaattcaga agtaatccct cattcccgct gtggccaaca
    20881 gcgggaatat cgttgcctct gtaccacata gattttaccc gccccctctc ctattctttt
    20941 cagtgtgctc tcaggaggtt gctatgtgtg gacgctttgc ccaatcccaa acgcgtgaag
    21001 attaccttgc gcttctcgcg gaagatattg aacgcgatat tccctatgat cccgaaccca
    21061 ttggcagata caacgtcgcg ccgggaacca aagttcttct gctcagtgaa cgtgatgaac
    21121 accttcatct ggatccggtt ttctggggat atgctcccgg atggtgggat aaaccgccgc
    21181 tgattaacgc ccgcgtagaa actgcggcca ccagtcgtat gtttaaaccg ctctggcaac
    21241 atggtcgggc aatctgtttt gccgatggct ggtttgagtg gaaaaaagaa ggcgacaaaa
    21301 aacagcctta ttttatctat cgtgctgacg gacaacctgt ttttatggcc gcgataggta
    21361 gcacaccgtt tgagcgcggt gacgaagccg aaggattttt aattgtcact gcagcagcag
    21421 accagggtct ggtagatatt catgaccgcc gcccgctggt actgtcgcca gaagctgcgc
    21481 gggaatggat gcggcaagag attggcggta aagaagcatc agaaattgct accaacggct
    21541 gtgtgccagc aaaccagttc acctggcacc cggtatcgcg cgcggtaggt aatgtcaaaa
    21601 accagggcgc ggagttaatt caaccggttt gataagccag gagactattg atcaataata
    21661 ctgcgtcata agaaatctct attagacaaa gtagacgctc cttccacatg cgccacaaga
    21721 aacctctatt ccagtgacac aattacgcct gattatttcc ctagcccgga atatgaataa
    21781 aaacatcaac aaagattaaa ttattcaaac gttactgttg cgtctgcggt cgtaacaaca
    21841 tccgacgtgg tgatgtgtat caaatgaggc ctctgatgat cagagggttt attgcgtata
    21901 cggatcgtgg atggcagaaa actttcagga tcaggtatcc attttaaacc agaaataggg
    21961 tgagtttgcc ccatccatgc cccacgcggg cagatgggat gtaattaaca acaggttaca
    22021 aaacctacac ttgcatggac atcacttcct gatacgccgc caccagctta ttacgcacct
    22081 gaatccccat ttgcatagaa actgaggctt tttgcatatc ggtcatcaca tcgtttaacg
    22141 ccacgccggg ttcaccgagg gtgaattttt ctgcctgcgt gcgggcagct gtttgtgtat
    22201 cgctaatgcg atcgagcgcg gcgtgcagct gcccggcaaa actaatggtc ggttgcggca
    22261 gtgaatcctg cgcgcgcgca ctcatcgccg tagcctgtaa ctggctgata accccttcaa
    22321 tcccctgtat cgctgacatt ttcatctcct ggataatttc tggtagcaaa gcctaccagt
    22381 aagtcaataa gacaaaggcg ctaaatagca acaaaaaaac gggtttattg gcggatagaa
    22441 aaaaacgaaa gcacaaataa tgggagcgtc aatttttcga gtttgctgac ccgggagtga
    22501 gtcttgttcc actttgccaa taacgccgtc cataatcagc cacgaggtgc gcgatgaatg
    22561 cgactgcagc ccagacaaaa tctcttgagt ggcttaatcg cctgcgtgcg aatccgaaaa
    22621 ttccattgat tgttgccggt tccgcggcag tggcggtcat ggtcgcactg atcctgtggg
    22681 cgaaagcccc cgactaccgc acattattca gcaatctttc cgatcaggat ggtggcgcaa
    22741 ttgtcagcca actgacgcaa atgaatattc cttaccgctt cagcgaagcc agcggcgcta
    22801 ttgaagttcc ggcagataaa gttcacgaac tgcgtctgcg cctggcacaa caaggtttgc
    22861 ccaaaggcgg cgcggtcggt ttcgaactac tcgatcagga aaagtttggt atcagccagt
    22921 tcagcgaaca ggtgaattat cagcgggcgc tggaaggcga gctttctcgt accatcgcaa
    22981 ctatcggccc ggtaaaaggg gcgcgcgtac atctggcaat gccgaaaccg tctttattcg
    23041 tccgtgaaca aaaatcccct tctgcatcgg tgacggtaaa tctgttaccc ggccgcgcac
    23101 tcgatgaagg gcaaattagc gccattgtgc atctggtttc cagcgccgtt gctggtctgc
    23161 cgccgggaaa cgtcacgctg gtggatcagg gcggacatct gttaacccag tccaatacca
    23221 gcgggcgcga tcttaatgat gctcagttga aatatgccag cgatgtcgaa ggccgtattc
    23281 agcggcgtat tgaagcaatc ctgtcgccta ttgttggtaa cggtaatatt cacgcccagg
    23341 tcacggcgca gctggacttc gccagtaaag aacaaacgga agaacagtat cgccctaacg
    23401 gtgatgaatc tcatgcggcg cttcgttcac gccagcttaa tgagagcgag caaagcggtt
    23461 ccggttatcc gggcggcgta ccgggggcgt tgtcgaatca accggcacct gcgaataacg
    23521 cgccaatcag cacgcctccg gcaaatcaaa ataaccgcca gcagcaggcg agcaccacca
    23581 gcaatagcgg gccgcgtagc acacagcgga atgaaaccag taactacgaa gtcgatcgca
    23641 ccattcgtca taccaaaatg aacgtgggcg atgtgcaacg tctgtcagtc gcggtagtgg
    23701 tgaattacaa aaccttgcca gacggtaaac cattgcctct cagcaacgaa cagatgaagc
    23761 aaattgaaga tctgacccgc gaggcgatgg gcttttctga aaaacgcggt gactcgctca
    23821 atgtcgttaa ctcgccgttc aatagcagtg acgaaagcgg cggagaactg ccgttctggc
    23881 aacagcaagc gtttatcgat cagttgctgg ctgccggtcg ctggttgctg gtactgctgg
    23941 tggcgtggct gctgtggcgg aaagcggtac gcccgcagtt aacacgtcgc gccgaggcga
    24001 tgaaagctgt acagcaacag gcgcaggccc gtgaggaagt ggaagatgcg gtggaagtcc
    24061 gcctgagcaa agacgaacaa ctccaacaac gacgcgctaa ccaacgtctg ggggcagaag
    24121 tcatgagcca gcgtatccgt gaaatgtctg ataacgatcc gcgcgtggtg gcgctggtca
    24181 ttcgccagtg gataaataac gatcatgagt aacctgacag gcaccgataa aagcgtcatc
    24241 ctgctgatga ccattggcga agaccgggcg gcagaggtgt tcaagcacct ctcccagcgc
    24301 gaagtgcaaa ccctgagcgc tgcaatggcg aacgtcacgc agatctccaa caagcagcta
    24361 accgatgtgc tggcggagtt tgagcaagaa gctgaacagt ttgccgcact gaatatcaac
    24421 gccaacgatt atctgcgttc ggtattggtc aaagctctgg gtgaggaacg tgccgccagc
    24481 ctgctggaag atattctcga aactcgcgat accgccagcg gtattgaaac gctcaacttt
    24541 atggagccgc agagtgccgc cgatctgatt cgcgatgagc atccgcaaat tatcgccacc
    24601 attctggtgc atctgaagcg cgcccaggcc gccgatattc tggcgttgtt cgatgaacgt
    24661 ctgcgccacg acgtgatgtt gcgtatcgcc acctttggcg gcgtgcagcc agccgcgctg
    24721 gcggaactga ccgaagtact gaatggcttg ctcgacggtc agaatctcaa gcgcagcaaa
    24781 atgggcggcg tgagaacggc agccgaaatt atcaacctga tgaaaactca gcaggaagaa
    24841 gccgttatta ccgccgtgcg cgaattcgac ggcgagctgg cgcaaaaaat tatcgacgag
    24901 atgttcctgt tcgaaaatct ggtggatgtc gacgatcgca gcattcagcg tctgttgcag
    24961 gaagtggatt ccgaatcgct gttgatcgcg ctgaaagggg ccgagcagcc actgcgcgag
    25021 aaattcctgc gcaatatgtc gcagcgtgcc gccgatattc tgcgcgacga tctcgccaac
    25081 cgtggtccgg tgcgtctgtc gcaggtggaa aacgaacaga aagcgattct gctgattgtg
    25141 cgccgccttg ccgaaactgg cgagatggta attggcagcg gcgaggatac ctatgtctga
    25201 taatctgccg tggaaaacct ggacgccgga cgatctcgcg ccaccacagg cagagtttgt
    25261 gcccatggtc gagccggaag aaaccatcat tgaagaggcc gaacccagcc ttgagcagca
    25321 actggcgcaa ctgcaaatgc aggcccatga gcaaggttat caggcgggga ttgccgaagg
    25381 tcgccagcaa ggtcatgagc agggctatca ggaaggactg gcccgggggc tggagcaagg
    25441 tctggcagag gcgaagtctc aacaagcgcc aattcatgcc cggatgcagc aactggtcag
    25501 cgaatttcaa actacccttg atgcgcttga tagtgtgatt gcttcgcgcc tgatgcagat
    25561 ggcgctggag gcggcacgtc aggtcatcgg tcagacgcca acagtggata actcggcact
    25621 gatcaaacag atccaacagt tgttgcagca agaaccgtta ttcagcggta aaccacagct
    25681 gcgcgtgcac ccggatgatc tgcaacgtgt ggatgatatg ctcggcgcaa ccttaagttt
    25741 gcatggctgg cgcttgcggg gcgatcccac actccatcct ggcggctgta aagtctccgc
    25801 cgatgaaggc gatctcgacg ccagtgtcgc cactcgctgg caagaactct gccgtctggc
    25861 agcaccagga gtggtgtaat gaccacgcgc ctgactcgct ggctaaccac gctggataac
    25921 tttgaagcca aaatggcgca gttgcctgcg gtacgtcgct atgggcgatt aacccgcgct
    25981 accgggctgg tgctggaagc caccggatta caattgccgc tcggcgcaac ctgtgtcatt
    26041 gaacgccaga acggcagcga aacgcacgaa gtagaaagcg aagtcgttgg ctttaacggt
    26101 caacggctgt ttttaatgcc gctggaggaa gtcgaaggtg tcctgcccgg cgcgcgggtt
    26161 tatgccaaaa acatttcggc tgaaggactg caaagcggca agcagttgcc gctcggtccg
    26221 gcgttattag gtcgcgttct ggacggcagc ggtaagccgc tcgatggcct gccctccccc
    26281 gatacgacgg aaaccggtgc gctgattacc ccgccattta acccgttgca acgtacaccg
    26341 attgaacatg tgctggacac cggcgtgcgc ccaatcaatg ccctgcttac cgttgggcgt
    26401 gggcagcgta tgggactgtt tgccgggtcc ggcgttggta aaagtgtgct gctggggatg
    26461 atggcacgtt acacccgcgc cgatgtcatt gtcgtgggtt tgattggtga acgcggacgc
    26521 gaagtaaaag attttattga gaatatcctc ggtgccgaag ggcgtgcacg ctccgtggtg
    26581 attgccgctc cggcggatgt ttctcctctc ctgcgaatgc agggtgccgc ctatgccacg
    26641 cgcattgccg aagattttcg cgatcgtggt cagcatgtgt tgctgattat ggactccctc
    26701 acccgctacg cgatggccca gcgtgaaatt gcgctggcga ttggcgaacc acccgccact
    26761 aaaggttatc ccccgtcggt gtttgccaaa ttaccggcac tggtcgagcg tgccggaaat
    26821 ggcattagcg gcggcggatc gattactgca ttttataccg tgctaactga aggcgatgac
    26881 cagcaggacc ccattgccga ctctgcgcgg gcaatcctcg acggtcacat tgtgctgtct
    26941 cgccgactgg cggaagccgg gcactatccg gctatcgata ttgaagcgtc gatcagccgc
    27001 gcaatgacgg cgttgatcag tgagcaacat tacgcgcgag tgcgcacctt caaacagctg
    27061 ttgtcgagtt ttcagcgtaa ccgcgatctg gttagcgtcg gcgcgtatgc caaaggcagc
    27121 gatccgatgc tcgacaaagc catcgccctg tggccgcagc tggagggcta tttgcaacaa
    27181 ggcatttttg aacgcgcgga ctgggaagcg tctctccagg ggctggagcg tattttcccg
    27241 acagtgtcat aacccaggag ataacggcag atggcagaac atggcgcgct ggcgaccctg
    27301 aaagatctgg cagaaaaaga ggtagaggat gccgcgcgcc tgctgggtga aatgcgtcgc
    27361 gggtgtcagc aggcggaaga acagctcaaa atgctgatcg attatcagaa tgaatatcgc
    27421 aataacctca acagcgatat gagtgccggg atgaccagca accgctggat caactatcag
    27481 cagtttatcc agacgctgga aaaagccatt actcagcatc gccagcaact taatcagtgg
    27541 acgcagaaag ttgacattgc cctgaacagc tggcgagaaa agaaacaacg tttgcaggcc
    27601 tggcagacac tgcaggaacg gcaatccacg gcggcactgc ttgcagaaaa ccgcctcgat
    27661 cagaaaaaga tggatgagtt cgcccagcgc gccgccatga ggaaacctga atgattcgct
    27721 tagcgccctt aattaccgcc gacgttgaca ccaccacatt gcctggcggc aaagccagcg
    27781 atgctgcaca agattttctc gcgttgttga gcgaagcatt agcaggcgag acaactaccg
    27841 acaaagcggc cccccagttg ctggtggcaa cagataagcc cacgacaaaa ggcgagccgc
    27901 tggtcagcga gattcttgcc gatgcgcaac aagcggattt actgatccct gtggatgaaa
    27961 caccgcctgt catcaacgac gaacaatcca catcaacacc attaaccacc gctcaaacga
    28021 tgacgttggc tgcggtggct ggcaacaata cggcaaaaga cgaaaaagcg gatgatctga
    28081 atgaagacgt caccgcaagc ctgagcgccc tttttgcgat gttgccgggt tttgacaata
    28141 cgcccaaagt gactgatgtg ccctcaaccg tgttaccggc agagaaacca acgctcttca
    28201 caaaactgac ttctgcgcaa ctcacaacag cacagcctga tgatgccccc ggcacgccag
    28261 ctcagccatt aacaccgctg gtagcagaag cccagagtaa agcggaagtc atcagcacac
    28321 cttcaccggt gaccgctgcc gccagcccgc taatcactcc acaccagaca cagccactgc
    28381 ccaccgtcgc cgcgcctgtg ttgagtgcac cgctgggttc tcacgaatgg caacaatcat
    28441 taagccagca tatttcgctg ttcacccgcc aggggcaaca aagtgcagag ttgcgtctgc
    28501 acccgcagga tttaggtgaa gtgcaaatct ccctcaaagt ggatgataac caggcgcaaa
    28561 tccagatggt ttcaccgcat caacacgtac gcgccgccct ggaagcagcg cttccggtac
    28621 tgcgaacgca gctggccgaa agtggcattc agttagggca aagcaacatc agtggcgaaa
    28681 gctttagtgg tcagcagcag gccgcttccc agcaacagca aagccaacgc acagcaaacc
    28741 atgaacctct ggcgggggaa gaagacgata cgcttccggt tcccgtctct ttacaagggc
    28801 gcgtaacagg caacagcggc gttgatattt tcgcctaacg tcagaggtag caccgtaatc
    28861 cgcgtctttt ccccgctttg ttgcgctcaa gacgcaggat aattagccga taagcagtag
    28921 cgacacagga agaccgcaac acatgactga ttacgcgata agcaagaaaa gcaagcgatc
    28981 gctttggatc ccgattctgg tattcattac cctcgcggcc tgtgccagcg caggttacag
    29041 ctactggcat tcgcatcagg ttgccgctga cgacaaagcg cagcaacgcg tcgtgccctc
    29101 cccggtcttc tatgcgctgg atacctttac ggtcaatctg ggcgatgcgg atcgcgtgct
    29161 gtatatcggc ataaccctgc gcctgaaaga tgaagctaca cgctcgcggc tgagtgagta
    29221 tttgccggaa gtccgtagcc gtttgctgtt actgttttcg cgtcaggatg ctgccgtact
    29281 ggcgacagaa gaaggcaaga aaaacctgat tgccgagatt aaaaccacgc tttccacccc
    29341 gcttgttgcc gggcaaccga aacaggatgt caccgacgtg ctgtataccg cttttattct
    29401 gcgataacga catgggcgat agtattcttt ctcaagctga aattgatgcg ctgttgaatg
    29461 gtgacagcga agtcaaagac gaaccgacag ccagtgttag cggcgaaagt gacattcgtc
    29521 cgtacgatcc gaatacccaa cgacgggttg tgcgcgaacg tttgcaggcg ctggaaatca
    29581 ttaatgagcg ctttgcccgc cattttcgta tggggctgtt caacctgctg cgtcgtagcc
    29641 cggatataac cgtcggggcc atccgcattc agccgtacca tgaatttgcc cgcaacctgc
    29701 cggtgccgac caacctgaac cttatccatc tgaaaccgct gcgcggcaca gggctggtgg
    29761 tgttttcacc gagtctggtg tttatcgccg tggataacct gtttggcggc gatggacgct
    29821 tcccgaccaa agtggaaggt cgcgagttta cccataccga acagcgcgtc atcaaccgca
    29881 tgttaaagct ggcgcttgaa ggctatagcg acgcctggaa ggcgattaat ccgctggaag
    29941 ttgagtacgt gcgttcggaa atgcaggtga aatttaccaa tatcaccacc tcgccgaacg
    30001 acattgtggt taacacgccg ttccatgtgg agattggcaa cctgaccggc gaatttaata
    30061 tctgtctgcc attcagcatg attgagccgc tacgggaatt gttggttaac ccgccgctgg
    30121 aaaactcgcg taatgaagat cagaactggc gcgataacct ggtgcgccag gtgcagcatt
    30181 cacagctgga gttggtcgcc aactttgccg atatctcgct acgcctgtcg cagattttaa
    30241 aactgaaacc cggcgacgtc ctgccgatag aaaaacccga tcgcatcatc gctcatgttg
    30301 acggcgttcc ggtactgacc agtcagtacg gcaccctcaa cggtcagtat gcgttacgga
    30361 tagaacattt gattaacccg attttaaatt ctctgaacga ggaacagccc aaatgagtga
    30421 catgaataat ccggccgatg acaacaacgg cgcaatggac gatctgtggg ctgaagcttt
    30481 gagcgaacaa aaatcaacca gcagcaaaag cgctgccgac gcggtgttcc agcaatttgg
    30541 cggtggtgat gtcagcggaa cgttgcagga tatcgacctg attatggata ttccggtcaa
    30601 gctgaccgtc gagctgggcc gtacgcggat gaccatcaaa gagctgttgc gtctgacgca
    30661 agggtccgtc gtggcgctgg acggtctggc gggcgaacca ctggatattc tgatcaacgg
    30721 ttatttaatt gcccagggcg aagtggtggt cgttgccgat aaatatggcg tgcggatcac
    30781 cgatatcatt actccgtctg agcgaatgcg ccgcctgagc cgttagtgat gaataaccac
    30841 gctactgtgc aatcttccgc gccggtttct gctgcgccac tgctgcaggt gagcggcgca
    30901 ctcatcgcca ttattgccct gatcctcgct gctgcctggc tggtaaaacg gttgggattt
    30961 gtccctaaac gcactggcgt taacggtctg aaaattagcg ccagtgcttc gctgggcgcg
    31021 cgtgaacggg ttgtggtggt cgatgtggaa gatgcacggc tggtgctcgg cgttaccgca
    31081 gggcaaatca atctgctgca taaacttccc ccttcagcac caacggaaga gataccgcag
    31141 cccgattttc agtcggtcat gaaaaacttg cttaagcgta gcgggagatc ctgatgcgtc
    31201 gtttgttgtc tgtcgcccct gtccttctct ggctgattac gcccctcgcc ttcgcgcaac
    31261 tgccgggtat caccagccag ccgctgcctg gcggtggaca aagctggtcg ctcccggtgc
    31321 agacgctggt gttcatcacc tcgttgacgt ttattccggc aattttactg atgatgacca
    31381 gtttcacccg catcattatt gtttttggtt tattgcgtaa cgcgctggga acaccctccg
    31441 cgccacctaa ccaggtattg ctggggttgg cgctgttttt gacctttttt attatgtcgc
    31501 cggtgatcga caaaatttat gtcgatgcgt accagccatt cagcgaagag aaaatatcaa
    31561 tgcaggaggc gctggaaaaa ggggcgcagc cgctgcgtga gtttatgctg cgtcagaccc
    31621 gtgaggcgga tttagggttg tttgccagac tggcgaatac tggcccgttg cagggacctg
    31681 aagccgtgcc gatgcgcatt ttgctcccgg cctacgtgac cagcgagttg aaaaccgcat
    31741 ttcagatagg cttcacgatt ttcatccctt ttttgattat cgacctggtg attgccagcg
    31801 tgttgatggc attggggatg atgatggtcc cccccgccac cattgctctg ccctttaaac
    31861 tgatgctgtt tgtactggtg gatggctggc aattgctggt cggttcgctg gcgcagagct
    31921 tttacagcta gagaggcaaa atgacacctg aatcggtcat gatgatgggg actgaagcga
    31981 tgaaagtcgc gctggcactg gctgccccgc tgttgctggt ggcgctggtc acgggcctta
    32041 tcatcagtat tttgcaggcc gccacgcaga tcaacgaaat gacgctgtcg tttattccaa
    32101 aaatcatcgc cgtatttatt gccattatta ttgccggacc gtggatgctc aatctgttgc
    32161 tggattacgt ccgcaccttg ttcactaacc tgccgtatat catcgggtag cccgtacaat
    32221 gatgcaggtg acaagcgatc aatggctttc ctggctaagt ctgtacttct ggccgttact
    32281 gcgcgtgctg gcgctcattt ctaccgcgcc aattctgagc gaacgcagcg tgccgaaacg
    32341 ggtaaaactg ggtctggcaa tgatgatcac gttcgctatt gccccatcat tacctgccaa
    32401 cgatgttcct gttttttcgt tctttgctct gtggctggcc gtgcagcaga tcctgatcgg
    32461 cattgcgctt ggtttcacca tgcaatttgc ctttgccgct gtgcgaaccg ctggcgaaat
    32521 tatcggtctg caaatggggc tgtcatttgc gacgtttgtc gatccggcca gccatctaaa
    32581 tatgcccgtt ctagcccgta tcatggatat gctggcgtta ctgctgttcc tgacatttaa
    32641 cggtcattta tggttgattt cactgctggt cgataccttt cacaccctgc caattggtgg
    32701 cgaaccgttg aacagcaatg cgtttctggc actcaccaaa gcagggagtt tgattttcct
    32761 taacgggctg atgctggcgt taccgctcat tactctgctg ctgacactga atctggcatt
    32821 aggtttactt aatcgtatgg ccccgcaatt atccattttt gttattggat ttccattaac
    32881 tctgactgtc ggtatctctt taatggcggc attaatgccg ttaattgcac ctttttgcga
    32941 acatttattc agtgagatgt ttaatttgct ggctgatatt attagtgaat tgccattaat
    33001 ataattccgt aacgtttatc atgttatcct aaggattatc cgaaaaataa tacctgcgaa
    33061 catcttccag gatactcctg tggcgaaata tttgttttaa gctcactcac atatcgcaac
    33121 atttacttta ctttaagaca attccaggca aattatacaa cactttacgg gatagtaagt
    33181 ccgcctgaaa aatcgcgaga gtggcgcatt aggtgaccca tgttgttccg tttagtcatg
    33241 atgaaatatt caggtaaggg gaattatcgt tacgcattga gtgagggtat gccatgtcaa
    33301 cgattattat ggatttatgt agttacaccc gactaggttt aaccgggtat ctgttgagta
    33361 gaggggttaa aaaaagagaa atcaacgaca ttgaaaccgt tgatgacctt gccatagctt
    33421 gtgattcaca gcgcccttca gtggtgttta ttaatgaggt ctgtttcatc catgatgctt
    33481 ctaacagtca gcacatcaag cacatcatta atcaacatcc caatacgtta tttatcgttt
    33541 ttatggcaat tgccaatgtt cattttgatg aatatctatt ggtcagaaaa aattt
//
LOCUS       DAZGSP010000050        32811 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZGSP010000050 DAZGSP010000000
VERSION     DAZGSP010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.050"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(40..201)
                     /gene="rsmS"
                     /locus_tag="ACHK74_002621"
     CDS             complement(40..201)
                     /gene="rsmS"
                     /locus_tag="ACHK74_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFP0221414.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(215..742)
                     /gene="priC"
                     /locus_tag="ACHK74_002622"
     CDS             complement(215..742)
                     /gene="priC"
                     /locus_tag="ACHK74_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFP0221415.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            812..1189
                     /gene="ybaN"
                     /locus_tag="ACHK74_002623"
     CDS             812..1189
                     /gene="ybaN"
                     /locus_tag="ACHK74_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFP0221416.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            1342..1893
                     /gene="apt"
                     /locus_tag="ACHK74_002624"
     CDS             1342..1893
                     /gene="apt"
                     /locus_tag="ACHK74_002624"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFP0221417.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            2022..3953
                     /gene="dnaX"
                     /locus_tag="ACHK74_002625"
     CDS             2022..3953
                     /gene="dnaX"
                     /locus_tag="ACHK74_002625"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFP0221418.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    3286..3350
                     /gene="dnaX"
                     /locus_tag="ACHK74_002625"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            4006..4335
                     /gene="ybaB"
                     /locus_tag="ACHK74_002626"
     CDS             4006..4335
                     /gene="ybaB"
                     /locus_tag="ACHK74_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFP0221419.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            4335..4940
                     /gene="recR"
                     /locus_tag="ACHK74_002627"
     CDS             4335..4940
                     /gene="recR"
                     /locus_tag="ACHK74_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFP0221420.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            5050..6924
                     /gene="htpG"
                     /locus_tag="ACHK74_002628"
     CDS             5050..6924
                     /gene="htpG"
                     /locus_tag="ACHK74_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFP0221421.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            7105..7749
                     /gene="adk"
                     /locus_tag="ACHK74_002629"
     CDS             7105..7749
                     /gene="adk"
                     /locus_tag="ACHK74_002629"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFP0221422.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            7985..8947
                     /gene="hemH"
                     /locus_tag="ACHK74_002630"
     CDS             7985..8947
                     /gene="hemH"
                     /locus_tag="ACHK74_002630"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFP0221423.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(8944..9903)
                     /gene="aes"
                     /locus_tag="ACHK74_002631"
     CDS             complement(8944..9903)
                     /gene="aes"
                     /locus_tag="ACHK74_002631"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFP0221424.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQSDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            10055..11359
                     /gene="gsk"
                     /locus_tag="ACHK74_002632"
     CDS             10055..11359
                     /gene="gsk"
                     /locus_tag="ACHK74_002632"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFP0221425.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(11492..13168)
                     /gene="ybaL"
                     /locus_tag="ACHK74_002633"
     CDS             complement(11492..13168)
                     /gene="ybaL"
                     /locus_tag="ACHK74_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFP0221426.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLMVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(13406..14626)
                     /gene="fsr"
                     /locus_tag="ACHK74_002634"
     CDS             complement(13406..14626)
                     /gene="fsr"
                     /locus_tag="ACHK74_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFP0221427.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKSKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            14844..16496
                     /gene="ushA"
                     /locus_tag="ACHK74_002635"
     CDS             14844..16496
                     /gene="ushA"
                     /locus_tag="ACHK74_002635"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP0221428.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(16533..17012)
                     /gene="ybaK"
                     /locus_tag="ACHK74_002636"
     CDS             complement(16533..17012)
                     /gene="ybaK"
                     /locus_tag="ACHK74_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFP0221429.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(17216..18010)
                     /gene="ybaP"
                     /locus_tag="ACHK74_002637"
     CDS             complement(17216..18010)
                     /gene="ybaP"
                     /locus_tag="ACHK74_002637"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFP0221430.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            18148..18489
                     /gene="ybaQ"
                     /locus_tag="ACHK74_002638"
     CDS             18148..18489
                     /gene="ybaQ"
                     /locus_tag="ACHK74_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP0221431.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(18603..21107)
                     /gene="copA"
                     /locus_tag="ACHK74_002639"
     CDS             complement(18603..21107)
                     /gene="copA"
                     /locus_tag="ACHK74_002639"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFP0221432.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            21369..22301
                     /gene="glsA"
                     /locus_tag="ACHK74_002640"
     CDS             21369..22301
                     /gene="glsA"
                     /locus_tag="ACHK74_002640"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFP0221433.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHERVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            22304..23596
                     /gene="ybaT"
                     /locus_tag="ACHK74_002641"
     CDS             22304..23596
                     /gene="ybaT"
                     /locus_tag="ACHK74_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP0221434.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            23627..23724
                     /locus_tag="ACHK74_002642"
                     /pseudo
     CDS             23627..23724
                     /locus_tag="ACHK74_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23721..24128
                     /gene="cueR"
                     /locus_tag="ACHK74_002643"
     CDS             23721..24128
                     /gene="cueR"
                     /locus_tag="ACHK74_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFP0221435.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(24129..24587)
                     /gene="ybbJ"
                     /locus_tag="ACHK74_002644"
     CDS             complement(24129..24587)
                     /gene="ybbJ"
                     /locus_tag="ACHK74_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFP0221436.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(24584..25501)
                     /gene="qmcA"
                     /locus_tag="ACHK74_002645"
     CDS             complement(24584..25501)
                     /gene="qmcA"
                     /locus_tag="ACHK74_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP0221437.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            25647..26324
                     /gene="fetA"
                     /locus_tag="ACHK74_002646"
     CDS             25647..26324
                     /gene="fetA"
                     /locus_tag="ACHK74_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFP0221438.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            26311..27090
                     /gene="fetB"
                     /locus_tag="ACHK74_002647"
     CDS             26311..27090
                     /gene="fetB"
                     /locus_tag="ACHK74_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFP0221439.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(27153..28007)
                     /gene="cnoX"
                     /locus_tag="ACHK74_002648"
     CDS             complement(27153..28007)
                     /gene="cnoX"
                     /locus_tag="ACHK74_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFP0221440.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(28068..28877)
                     /gene="ybbO"
                     /locus_tag="ACHK74_002649"
     CDS             complement(28068..28877)
                     /gene="ybbO"
                     /locus_tag="ACHK74_002649"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP0221441.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(28867..29490)
                     /gene="tesA"
                     /locus_tag="ACHK74_002650"
     CDS             complement(28867..29490)
                     /gene="tesA"
                     /locus_tag="ACHK74_002650"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFP0221442.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            29461..30147
                     /gene="ybbA"
                     /locus_tag="ACHK74_002651"
     CDS             29461..30147
                     /gene="ybbA"
                     /locus_tag="ACHK74_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFP0221443.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            30144..32558
                     /gene="ybbP"
                     /locus_tag="ACHK74_002652"
     CDS             30144..32558
                     /gene="ybbP"
                     /locus_tag="ACHK74_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFP0221444.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLLVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMMLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVEPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         7801 a         8631 c         8813 g         7566 t
ORIGIN      
        1 ccacaatctt aggggacatt tagcggcccc atttatttct cacttttccg cctcatcatc
       61 gcgcgttaat ttcttttcat aatcgcgttt tacaatatcc agcgcgcgca gcacggtgcg
      121 ggctgggatc tgattttctt ccagcagcac aatcaaatcg acagccagtt tgacatcgtc
      181 aggggcattt tccagtgaca tactctctcc attgctagcg ggttaagcgc gctagcctgt
      241 tttcgatttt ttccagcgca tggcggcagc gtgccaggcg cgcttcatag gcttccactt
      301 cacgatgcag cgtttgctgt tccacgagat cggtcacccg cgccaggcgg gctctgcgtt
      361 cggcaaccat ctcacgtagc cgccgctcaa aatcctgatg ctgaatacgt ttacgctgcc
      421 agcgggcaat tttcggtggc gcactgtccc actcgcgcaa tgaccaggcg gaggcttcac
      481 gcgcgatggc ttccagttgt gccgccagat gttccgccag ccaggccact tgcggcagct
      541 gttgctgctc aactgcatga cgaagcgcag ccagattatc gcccgcctca tcgagacaag
      601 cctgcagtgt tgtcgcacgg gtttgaaaaa ggtgtctgtc aaaacgggca cttagcgtgg
      661 caaattgcgc taccggggca caacgctgac gcagcgtagc gagctgtcct tccagttttt
      721 ccagcagcag ggcggttttc acgataagac ctcaatgaaa atgataattg ttatgctaaa
      781 gtagccactc tgtaagctga caacactcaa tatgcaacga atcattttaa tcatcattgg
      841 ctggctggcg gtagtgctgg gtacgctggg cgtggtatta ccggtattac cgacgacgcc
      901 gtttatcctg ctggcggcct ggtgctttgc ccgttcttcc ccgcgctttc acgcctggtt
      961 gctgtaccgc tcatggtttg gcagctatct acgtttctgg cagaaacatc atgcgatgcc
     1021 gcgcggcgtc aaaccgcggg cgattttgct tattttgctc acgtttgcca tttctctgtg
     1081 gttcgtccag atgccatggg tgcgcatcat gttgctggta attctcgcct gtttgctttt
     1141 ctatatgtgg cgaattccgg tgattgatga aaagcaagaa aagcactgaa gcacaacaat
     1201 cgcagttgca attattgcgt acagccagta cattctggcg ttttcgagca caggcgcagg
     1261 cggtcaaagg ttaaacaact gttacttttg atacgtttaa aacgcgccgt gagtaccacc
     1321 gtaacaagca ggcatacact tatgaccgcg actgcacagc agcttgagta tctcaaaaat
     1381 agcatcaaaa gcattcagga ctacccaaaa cccggcattc ttttccgcga tgtcaccagc
     1441 ttactggaag acccgaaagc ttacgctctc agcatcgact tgctggttga gcgttacaaa
     1501 aatgcgggca ttaccaaagt tgtcggcacc gaagcgcgtg gcttcttgtt tggcgctccg
     1561 gtagctctgg gtctgggcgt tggctttgta ccggtccgta aaccgggcaa actgccgcgt
     1621 gaaaccatca gtgaaactta cgacctggaa tacggcaccg atcagctgga gatccacgtt
     1681 gatgccatca aaccgggcga caaagttctg gtggtggacg atctgctggc aaccggcggc
     1741 actatcgaag cgaccgttaa actgatccgt cgtctgggtg gtgaagtggc tgacgctgcg
     1801 ttcattatca acctgttcga tctcggcggc gaacagcgtc tcgaaaaaca gggcattacc
     1861 agctacagcc ttgtcccgtt cccgggccat taattatcgc cagtcttgtg ctgcccacgc
     1921 tacggacagc acaagatgtg cattcagcct cgccgttctg acggggctgt gttagcatta
     1981 ccccttcgtg aatccacctt ccagcgtttc agagcctgcc aatgagttat caggtcttag
     2041 cccgaaaatg gcgcccacaa acctttgctg acgtcgtcgg ccaggaacat gtgctgaccg
     2101 cactggcgaa cggcttgtcg ttagggcgta ttcatcatgc ttatcttttt tccggcaccc
     2161 gtggcgtcgg aaaaacctct atcgcccgac tgctggcgaa ggggctaaac tgcgaaaccg
     2221 gcattaccgc gacgccgtgc ggcgtgtgcg ataactgtcg tgaaatcgag caggggcgct
     2281 ttgtcgatct gattgaaatc gacgccgcct cgcgcaccaa agttgaagat acccgcgacc
     2341 tgctggataa cgtccagtac gctccggcgc gtggtcgttt caaagtttat ctcatcgacg
     2401 aagtgcatat gctgtcgcgc cacagcttta acgcactgtt aaaaactctt gaagagccgc
     2461 cggagcatgt taagtttctg ctggcgacga ccgatccaca gaaattgccg gtgacgattt
     2521 tgtcacgctg tctgcaattt catctcaagg cgctggatgt cgagcaaatt cgccatcagc
     2581 ttgagcacat cctcaacgaa gaacatatcg ctcacgagcc gcgggcgctg caattgctgg
     2641 cacgcgccgc tgaaggcagc ctgcgagatg ccttaagtct gaccgaccag gcgattgcca
     2701 gtggtgacgg ccaggtttca acccaggcgg tcagtgcgat gctgggtacg cttgacgacg
     2761 atcaggcgct gtcgctggtt gaagcgatgg tcgaggccaa cggcgagcgc gtaatggcgc
     2821 tgattaatga agccgctgcc cgtggtatcg agtgggaagc gttgctggtg gaaatgctcg
     2881 gcctgttgca tcgtattgcg atggtacaac tttcgcctgc tgcacttggc aacgacatgg
     2941 ccgccattga gctgcggatg cgtgaactgg cgcgcaccat accgccgacg gatattcagc
     3001 tttactatca gacgctgttg attggtcgca aagaattacc gtatgcgccg gaccgccgca
     3061 tgggcgttga gatgacgctg ctgcgcgcgc tggcgttcca tccacgtatg cctctgcctg
     3121 agccagaagt gccacgccag tcctttgcgc ccgtcgcgcc aacggcagta atgacgccaa
     3181 cccaggtgcc gccgcaaccg caatcagcgc cgcagcaggc accgactgta ccgctcccgg
     3241 aaaccaccag ccaggtgctg gcggcgcgcc agcagttgca gcgcgtgcag ggagcaacca
     3301 aagcaaaaaa gagtgaaccg gcagccgcta cccgcgcgcg gccggtgaat aacgctgcgc
     3361 tggaaagact ggcttcggtc accgatcgcg ttcaggcgcg tccggtgcca tcggcgctgg
     3421 aaaaagcgcc agccaaaaaa gaagcgtatc gctggaaggc taccactcca gtgatgcagc
     3481 aaaaagaagt ggtcgccacg ccgaaggcgc tgaaaaaagc gctggaacat gaaaaaacgc
     3541 cggaactggc ggcgaagctg gcggcagaag ccattgagcg cgacgcgtgg gcggcacagg
     3601 tgagtcaact ttcgctacca aaactggtcg aacaggtggc cttaaatgcc tggaaagagg
     3661 agagcgacaa cgcagtatgt ctgcatttgc gctcctctca gcggcatttg aacaaccgcg
     3721 gtgcacagca aaaactggct gaagcgttga gcatgttaaa aggttcaacg gttgaactga
     3781 ctatcgttga agatgataat cccgcggtgc gtacgccgct ggagtggcgt caggcgatat
     3841 acgaagaaaa acttgcgcag gcgcgcgagt ccattattgc ggataataat attcagaccc
     3901 tgcgtcggtt cttcgatgcg gagctggatg aagaaagtat ccgccccatt tgatcgtaag
     3961 cacagcttac gttcgtcatc cttaacgtga ttgagagaga aacctatgtt tggtaaaggc
     4021 ggtctgggta acctgatgaa gcaagcccag cagatgcaag aaaaaatgca gaaaatgcag
     4081 gaagagatcg cgcagctgga agtcaccggc gaatctggcg caggtctggt aaaagtgacc
     4141 atcaacggtg cacacaactg ccgtcgcgta gagatcgacc cgagcctgct ggaagacgac
     4201 aaagagatgc tggaagacct ggtggctgca gcattcaacg acgcagcacg tcgtattgaa
     4261 gaaacgcaga aagaaaaaat ggcctctgta tcctccggaa tgcagctgcc gcctggcttt
     4321 aagatgccgt tctgatgcaa accagcccgc tgttaacaca gcttatggaa gcactgcgct
     4381 gtctgccggg cgttggcccg aagtcggcgc agcgtatggc gttcacgctg cttcagcgcg
     4441 atcgtagcgg cgggatgcgt ctggcgcagg cgctcacccg ggcgatgtcg gaaatcggcc
     4501 actgcgccga ttgccgcact ttcaccgaac aggaagtctg taacatctgt tcgaatccgc
     4561 gtcgtcagga aaacggtcaa atctgtgtgg tggagagtcc ggcggacatt tacgccattg
     4621 agcagacggg gcagttttca ggtcgttatt ttgtgttgat ggggcacctg tcaccgctgg
     4681 acggcatcgg tccggatgat atcgggcttg atcgtctgga acagcgtctg gcagaggaaa
     4741 aaatcactga agtgatcctc gccaccaatc ccacggttga aggtgaagct accgctaact
     4801 acattgccga gctttgcgcg caatatgacg tggaagccag ccgaatcgct catggcgtac
     4861 cggttggcgg cgaactggaa atggtcgatg gcaccacgct gtcacactcc cttgccgggc
     4921 gtcataagat tcgtttttaa gcaaacgaga gcaggatcac ctgctctcgc ttgaaattat
     4981 tctcccttgt ccccatctct ccctcatcct gtttttaacc ttaaaatggc attattgagg
     5041 tagacctaca tgaaaggaca agaaactcgt ggttttcagt cagaagtgaa acagcttctg
     5101 cacctgatga tccattctct ctattccaat aaagaaatct tcctgcgtga gcttatctct
     5161 aacgcctccg atgcggcgga caagctgcgt ttccgtgcgc tatctaaccc ggacctgtac
     5221 gaaggtgatg gcgaactgcg cgttcgtgtc tctttcgata aagacaagcg cacgctgacc
     5281 atctccgata acggcgtggg gatgacccgc gacgaagtga ttgaccatct ggggactatc
     5341 gctaaatccg gtaccaaatc attcctcgaa tccctgggtt ctgaccaggc gaaagacagc
     5401 cagctgatcg gtcagttcgg tgttggtttc tactctgcgt ttatcgtggc cgacaaagtg
     5461 accgtgcgta ctcgcgcggc aggcgaaaaa ccagaaaatg gcgtgttctg ggaatcggct
     5521 ggcgaaggtg aatacaccgt tgccgacatc accaaagaag atcgtggtac tgaaatcacc
     5581 ctgcatctgc gtgaaggcga agacgagttc ctcgatgact ggcgcgtgcg ttccatcatt
     5641 agcaaatact ccgaccatat cgcgctgccg gtagagatcg aaaaacgcga agagaaagac
     5701 ggcgaaaccg ttatctcctg ggaaaaaatc aacaaagcgc aggcgctgtg gactcgtaac
     5761 aagtcggaaa tcaccgatga agagtacaaa gagttctaca aacacatcgc ccacgacttt
     5821 aacgatccgc tgacctggag ccacaaccgt gttgaaggta agcaggagta caccagcctg
     5881 ctgtacattc cgtcccaggc tccgtgggat atgtggaacc gcgatcataa acacggcctg
     5941 aaactgtacg tccagcgtgt gttcatcatg gacgacgcag aacagttcat gccgaactat
     6001 ctgcgcttcg tgcgtggtct gattgactcc agcgatctgc cgctgaacgt ttcccgtgaa
     6061 atcctccagg acagcacggt aacgcgtaac ctgcgcaatg cgctgaccaa gcgtgtgctg
     6121 caaatgctgg aaaaactggc gaaagacgac gcggaaaaat accagacctt ctggcaacag
     6181 tttggcctgg tactgaaaga aggtccggcg gaagatttcg ctaaccagga agcgatcgcc
     6241 aaactgctgc gttttgcttc tacttatacc gattcttctg cgcagaccgt gtctctggaa
     6301 gactacgttt cccgcatgaa agaagggcag gagaaaatct actacatcac tgcagacagc
     6361 tatgcggcag cgaagagcag cccgcacctg gaactgctgc gtaagaaagg catcgaagtt
     6421 ctgctgcttt ccgaccgcat cgatgagtgg atgatgaact atctgactga gttcgacggt
     6481 aaaccgttcc agtcggtgtc taaagttgac gagtcgctgg aaaaactggc tgacgaagtt
     6541 gatgagagcg cgaaagaagc ggagaaagca ctgactccgt tcatcgaccg tgtgaaagcc
     6601 ctgctcggcg agcgcgtgaa agatgtccgt ctgactcacc gtctgaccga tacgccagcg
     6661 atcgtttcga ccgacgcgga cgaaatgagc actcagatgg cgaaactgtt cgctgcggcg
     6721 ggccagaaag tgccagaagt gaaatacatc ttcgaactga acccggatca cgtactggtg
     6781 aaacgtgcgg cagatactga agatgaagcg aagttcagcg agtgggtaga actgctgctg
     6841 gatcaggcgc tgctggcaga acgcggcacg ctggaagatc cgaacctgtt tattcgtcgt
     6901 atgaaccagc tgctggtttc ctgatgtcat gccggatgac ctttgtgtca tccggcattt
     6961 ttcttttcat catctgcact ttccgcaaat tatctcgcca ttaaccgttt cagcctcagg
     7021 tgcctttctt gaggcaatcg cctgttggtg gtatcgttta tcgctttttc aaaaaattcg
     7081 acacatttta aggggatttt cgcaatgcgt atcattctgc ttggcgctcc gggcgcgggg
     7141 aaagggactc aggctcagtt catcatggag aaatatggta ttccgcaaat ctccactggc
     7201 gatatgctgc gtgctgcggt caaatctggc tccgagctgg gtaaacaagc aaaagacatt
     7261 atggatgctg gcaaactggt caccgacgaa ctggtgatcg cgctggttaa agagcgcatt
     7321 gctcaggaag actgccgtaa tggtttcctg ttggacggct tcccgcgtac cattccgcag
     7381 gcagacgcga tgaaagaagc gggcatcaat gttgattacg ttctggaatt cgacgtaccg
     7441 gacgaactga ttgttgatcg tatcgtaggc cgccgcgttc atgcgccgtc tggtcgtgtt
     7501 tatcacgtta aattcaatcc gccgaaagta gaaggcaaag acgacgttac cggtgaagaa
     7561 ctgactaccc gtaaagacga tcaggaagaa accgtgcgta aacgtctggt tgaataccat
     7621 cagatgactg caccgctgat cggctactac tccaaagaag cggaagcggg taacaccaaa
     7681 tacgcgaaag ttgacggcac caagccggtt gctgaagttc gcgctgatct ggaaaaaatc
     7741 ctcggctaat tcgaaagcgc gcacggacag tcccctcgcc ccctcgggga gagggttagg
     7801 gtgaggggaa caggcccgca caagcaaact tatcagcaat ctcaggccgg atattcattc
     7861 ggccttttac aaaaacctca atctgcgcta ttcctttttc tgatttgacc tctcacagca
     7921 attagttctt cttcctcact tttctgctac aattatcaac aagttgaatc gataagaggc
     7981 ggtaatgcgt cagactaaaa ccggtatcct gctggcaaac ctgggtacgc ccgatgcccc
     8041 cacacctgaa gcggtaaaac gctatctgaa acaattttta agcgacagac gcgtggttga
     8101 tacctcacgg ttgttatggt ggccgttgct gcgcggcgtg attttgccgc tgcgctcgcc
     8161 gcgtgtggcg aagctgtacg cctcggtctg gatggaagac ggctcgccgc tgatggttta
     8221 cagccgccag caacagcagg cgttggcaca acgtttaccg gatacgcccg tagcgctggg
     8281 catgagctac ggctcgccat cactggaaag cgccgtcgat gaactcctgg cagagcatgt
     8341 agatcatatt gtggtgctgc cgctttatcc gcaattctcc tgttctacgg tcggtgcggt
     8401 atgggatgaa ctggcacgca ttctggcgcg caaacgtagc attccgggga tatcgtttat
     8461 tcgtgattac gccgataacc acgattacat taatgcactg gcgaacagcg tacgcgcttc
     8521 ttttgccaaa catggcgaac cggatctgct gctgctctct tatcatggca ttccccagcg
     8581 ttatgcagat gaaggcgatg attacccgca acgttgccgc acaacgactc gcgaactggc
     8641 ttccgcactg gggatggcac cggaaaaagt gatgatgacc tttcagtcgc gctttggccg
     8701 ggaaccctgg ctgatgcctt ataccgacga aacgctgaaa atgctcggag aaaaaggcgt
     8761 aggtcatata caggtgatgt gcccgggctt tgctgcggat tgtctggaga cgctggaaga
     8821 gattgccgag caaaaccgtg aggtcttcct cggtgccggc gggaaaaaat atgaatatat
     8881 tccagcgctt aatgccacgc cggaacatat cgaaatgatg gctaatcttg ttgccgcgta
     8941 tcgctaaagc tgagcggtaa agaactgagc gccgtcgcga agagcctcgt cggcggtttt
     9001 catcattcgt gaataatgca aaaaggcatg cagcgtacct gggtagagtt tgaactcaca
     9061 gggctgctga tgcgccgcta acgtctggta aagcagacgg ctgtcatcca gcagcggatc
     9121 gaactccgcc ccggcaataa aacagggcgg aacttcgcga gtgagatcat tattaaacag
     9181 acagtaatac ggtgactcgc ggtccgcgtc gttgcttaaa tacgcctctt catacatctg
     9241 caaatcctgt tgcgttaagc catcccagac accgcccaac agacgacgag tcacggaatc
     9301 ccgtaatccg taaagcccat accacagcaa aacacccgca attttaccgc aatcgatctg
     9361 tttatcacgc aaccacaacg cactggcgag cgccagcatg gcacctgcgg aatcaccggc
     9421 aaagccaatg cgggacatat tgatttgata atcctccgcc tgctggtgga aataacaaca
     9481 agcagccaca atttcctcta tcgcttgcgg aaaacgcgct tcaggtgaaa gggggtaatc
     9541 aataccaatc accgtacatt ggctgtagct tgccagcagg cgcatgatgc gatcgtgggt
     9601 atcgagattg ccgagaataa aaccgcctcc atgcaaataa aatagcgtcg ccgggctatc
     9661 tggctgcgga caaaagagac gtgtttccac ctgcccatat tttgttggaa ccatgtaagc
     9721 tctggttgcc atttctggag cgcccgcatt ccagaatcgg cgctcaagcg tgtaatactg
     9781 tcgttgctca gcaatcgttc ccgttgcggg ccaggacggt aaatccgact gaagagtatt
     9841 cacaacggtc ttcatttcag cagaaataag gtccagaaca ggtagtttgt tttccggctt
     9901 catacaaaaa ctcctttcaa attacgtcat tgtaaggaac cactgccatg aaaatgcgat
     9961 cccgcctgct gatattgaaa ctggctgcgt ctcgcgcgct cccgtcagat tgtgttaaca
    10021 ttcgccgctc agttaaccac ccgtaaaaac aaccatgaaa tttcccggta aacgtaaatc
    10081 caaacattac ttccccgtaa atgcacgcga tccgctgctt cagcaattcc agccagaaaa
    10141 cgaaaccagc gctgcctggg tagtgggtat cgatcaaacg ctggtcgata ttgaagcgaa
    10201 agtggatgat gaattcattg agcgttatgg attaagcgcc gggcattcac tggtgattga
    10261 ggatgatgta gccgaagcgc tttatcagga actaaaacag aaaaacctga ttacccatca
    10321 gtttgcgggt ggcaccattg gtaacaccat gcataactac tcggtgctcg cggacgaccg
    10381 ttcggtgctg ctgggcgtca tgtgcagcaa tattgaaatt ggcagctatg cctatcgtta
    10441 cctgtgtaac acctccagcc gtaccgatct taactatcta caaggcgtgg atggcccgat
    10501 tggtcgttgc tttacgctga ttggcgagtc cggggaacgt acctttgcta tcagccctgg
    10561 ccacatgaac cagctgcggg ctgaaagtat tccggaagat gtgattgccg gagcctcggc
    10621 tctggttctc acctcttatc tggtgcgttg caagccgggt gaacccatgc cggaagcaac
    10681 catgaaagcc attgagtacg cgaagaaata taacgtaccg gtggtgctga cgctgggaac
    10741 taagtttgtc attgccgaga atccgcagtg gtggcagcaa ttcctcaaag accacgtctc
    10801 tatccttgcg atgaacgaag atgaagccga agcgttgacc ggagaaagcg atccgttgtt
    10861 ggcatctgac aaggcgctgg actgggtaga tctggtgctg tgcaccgccg ggccaatcgg
    10921 cttgtatatg gcgggcttta ccgaagacga agcgaaacgt aaaacccagc atccgctgct
    10981 gccgggcgct atagcggaat tcaaccagta tgagtttagc cgcgccatgc gccacaagga
    11041 ttgccagaat ccgctgcgtg tatattcgca cattgcgccg tacatgggcg ggccggaaaa
    11101 aatcatgaac actaatggag cgggggatgg cgcattggca gcgttgctgc atgacattac
    11161 cgccaacagc taccatcgta gcaacgtacc aaactccagc aaacataaat tcacctggtt
    11221 aacttattca tcgttagcgc aggtgtgtaa atatgctaac cgtgtgagct atcaggtact
    11281 gaaccagcat tcacctcgtt taacgcgcgg cttgccggag cgtgaagaca gcctggaaga
    11341 gtcttactgg gatcgttaag ttatcgtcgg ttcgtaggcc agataaggcg ttcacgccgc
    11401 atctggcatt tggctctcga tgcccgatgc gacgctggcg cgtcttatca tgcctacata
    11461 tttttcatat tttacatccg gcaaccaccg tttaccccgt caccacttca cccgccggtg
    11521 gcgtttccag cagttccagc atagtacggg caatttcacg ttcacccatc actacctgat
    11581 tcgcgccacg ttcggtgata tacgtcactt catcgtcata atgggcgcgg gcgataatct
    11641 caatatccgg gtttttcgcg cgggcagatg ccacaatctc gcctgcttca taaccgttgg
    11701 gaatcgtcag gatcagccat tttgcacatt ccagatgcgc cagttgcata atttcttcgt
    11761 tcgccgcatt gcccaatact gcgcggaccc cacgctctcg cagctcatca acacgggttc
    11821 gtgacgtctc aatcaccatc agcggaatat cagaggcgag caatttctcc cccagcaggc
    11881 tgcctacacg accgtaaccc accagtagcg catggttgca aatatccact gggatctgct
    11941 tctcttcttc gattgcctct tccagcgtct gctcttccag cgtttcggtc ttcgccagat
    12001 atttctccag tagtgcgaac agtaccgggt tgagcataat cgacaggatc gcccctgcca
    12061 gtaccaggtt ttgtccggcc tgcggcagta aattcaatgc cattcccagt cccgccagga
    12121 taaacgcgaa ctcaccaatc tgcgccaggc tggcggcgat ggttaatgcc gtacgttggg
    12181 agtgaccaaa cagtcgcacc aggaaaaatg cggctaacga cttaccaaac agaataatcg
    12241 ccagcgtcgc cagcactgcc agcggttgct gaatcagaat taacggatca aacaacatcc
    12301 cgacggagac aaaaaacagc accgcaaacg cgtcgcgcaa tggcagcgta tcgtgggcgg
    12361 cacggtgact cagttcagac tcgttcagta ccatcccggc aaagaacgca ccgagtgcaa
    12421 aggagacatc aaacagctct accgcaccaa aggcaatccc taacgccagc gccagcaccg
    12481 acagggtaaa cagctcgcga gaaccggttg ccgcgctgcg tgccataatc cacggcacca
    12541 gacggcgacc taccagcatc ataatggcga taaatgcgat cactttgccg atggtgatcc
    12601 ccatatcgac tgcaagagtg gcaaagccca catcgccctg ttccatcatt cctgccactg
    12661 cgggcagcaa caccagcgtc agaaccatta ccaggtcttc cacaatcaac caaccgatgg
    12721 cgatttgccc acgctgactg tcaattaatt gccgttcttc aagtgcgcgc agtaacacca
    12781 cggtactggc ggtggaaaga cataaaccga acacgatacc ggtcattaac gaccagccca
    12841 gcacggcaga gagcgccata cccagcagcg tcgccacggc tatctgggcg atcgcaccgg
    12901 gaatggcgat ggcctttacc gccatcaaat ccttcagcga aaagtgcaaa ccgacgccaa
    12961 acatcaacag aatgacgccc agttcagcca gttccggcgc aagcttggta tcggcaacaa
    13021 agcccggagt gaatggtcct gccagcacac ccgctaacag atatcccacc agaggagaaa
    13081 tacgtagttt attggccagc atgccgagga taaaggcgag cacaaggccg ccaacaatgg
    13141 tggtgataag cggggtggcg tgatgcattc cgtctccttt tcctggtggt tattgtccat
    13201 ttttggccgg gaaaaccaaa attacaggta atagtttatg acaatttcat tgatgatgtt
    13261 catgaataat tattgaattt tgcagaaaaa tggaattagc tgcaaaaaaa gcacggatcg
    13321 gaaaacagaa ggcggtttaa cagaggaaag gcttatggcg actgtaaaac gatgcagcca
    13381 aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg gttatcaggc aggaatatgg
    13441 tcaacatccc caatagtggc aggaaagcac agattttata gactaactcg atgctggtgt
    13501 gatcggcgat aagccccaga actgccgctc ccagacctcc catgccaaaa gcaaaaccga
    13561 aaaagagtcc agaaaccata ccgatacgtc ccggaagtag ctcctgagcg tagaccagaa
    13621 tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc cccgtccagt
    13681 gcaggctggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata gagccccaaa
    13741 tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta cctgccgcaa
    13801 cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat ccgaattttt
    13861 gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat ttcgagaaaa
    13921 tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt ggattgatta
    13981 tcgtcgcttt ggattttcct ttattcattc ggtgctgtgc cgagtaccaa cggctaattt
    14041 gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg ttgcctttgc
    14101 cataaggcgc gataatcacc gccgccagca agggtcccag ggaactgcca aagttgccgc
    14161 cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata cgggctacgc
    14221 gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgcc gccagcagaa
    14281 ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc acttaaggta aagcacatgc
    14341 caattggcaa cgaccacggc atcggatatt tatcggtcca gtagccgacc actggttgca
    14401 gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata aatgtcagag
    14461 aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc atgtcgttca
    14521 gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc ttggtcgttg
    14581 acgcagccgc gcccgccaca ggctggggtt gttcactcat tgccatagga aagtcacttt
    14641 ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact aacatacctg
    14701 tatgcgtcgc ctgaaggaag tctcaacgcc gaatacagaa tttctaatct ggatgcagat
    14761 ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt tcaacacgtt
    14821 gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg ttagcgctgt
    14881 taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat aaaacctaca
    14941 aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat gaatatggcg
    15001 aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag gttgcggctg
    15061 aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg cccgagtctg
    15121 acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat gacgcgatgg
    15181 cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag gaaaagtggg
    15241 ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag cgcctgttta
    15301 aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgtcattggg ctgacaaccg
    15361 atgacacagc aaaaattggt aacccggaat acttcactga tgtcgaattt cgtaagcccg
    15421 ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca gacattatta
    15481 tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac gcaccgggcg
    15541 atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc ggtggtcact
    15601 cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac gtgccgggta
    15661 cgccatgcaa gccagatcaa caaaacggca tctggattgt gcaggcgcat gagtggggca
    15721 aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg gttaactacc
    15781 agctgattcc ggtgaacctg aagaagaaag tgacctggga agacgggaaa agcgagcgcg
    15841 tgctttacac tcctgaaatc gctgaaaacc agcaaatgat ctcgctgtta tcaccgttcc
    15901 agaacaaagg caaagcgcag ctggaagtga aaataggcga aaccaatggt cgtctggaag
    15961 gcgatcgtga caaagtgcgt tttgtacaga ctaatatggg gcggttgatt ctggcagccc
    16021 aaatggatcg cactggtgcc gactttgcgg tgatgagcgg aggcggaatt cgtgattcta
    16081 tcgaagcagg cgatatcagc tataaaaacg tgctgaaagt gcagccattc ggcaatgtgg
    16141 tggtgtatgc cgacatgacc ggtaaagagg tgattgatta cctgaccgcc gtcgcgcaga
    16201 tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg gcgaaagacg
    16261 gcaaactgaa cgaccttaaa atcaaaggcg aaccggtcga tccggcgaaa acttaccgta
    16321 tggcgacatt aaacttcaat gccaccggcg gtgatggata tccgcgcctt gataacaaac
    16381 cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat atccagaaaa
    16441 gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg cagtaatccg
    16501 aaagtgccgg atgtttgcat ccggcacaat gcttaatcgc ggcgggcgat atcagcaaat
    16561 ttggcatcga ggatctttgc cagatcgcct gccgccagtt cgatatccag tccgcgcttg
    16621 ccgccggaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat cgttggcaga
    16681 cgttttttct gccccagtgg gctaatcccc ccaaccaggt atcccgtcga acgctgcgcg
    16741 accatcggat cggccatctc aactttcttg gcacccagcg cttttgctac ttttttaaga
    16801 tccagttgac ctgcgaccgg cgtaacggcc acggcaaggt gtttcatatc accgttcact
    16861 gccaccagca gcgttttgta gacctgatcc ggattcaaac ctaatttttt gacgacttca
    16921 tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa cgaaatcttg
    16981 tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta aacaaaaaaa
    17041 cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc taagagtatt
    17101 ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt gacggcataa
    17161 taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca tttttttagc
    17221 gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc gcgaccacat
    17281 atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc tgatgcatca
    17341 gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta ttttgcggcg
    17401 gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta tgccagtggg
    17461 tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac atggcaatct
    17521 ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc gcctgcaata
    17581 actggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc gtcgcctgaa
    17641 gaaccatcgc gatttgccac agcggttgag tagaaaagag cgacggagaa atgcccatct
    17701 cctggctaat gtgctgcaga ttttggagtt gctcctcgtt aatgcgctct tccagcgcct
    17761 cgcaagtagg caaattagca aaaggcgtat cgctggtgga aacatccgcc tcgacgatca
    17821 gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc atatcgtggc
    17881 tacccatatg aatactgcca atcagatgaa aatggcgatt gccgggaagg gtgatatcga
    17941 tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt acccggtaca
    18001 acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga gggatggcgc
    18061 aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct tgcatcgcta
    18121 ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc gacgacaccc
    18181 ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa tgagttagca
    18241 gagttgctgc atgttcatcg taatagcgtc agtgcactga tcaataacaa tcgtaaactc
    18301 accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga ttttaggcta
    18361 aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac ccaggaagaa
    18421 ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg tgcaaaaaag
    18481 gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt tacgccgcat
    18541 ccggcagtca tgcgtcgatg ccaaatgtgc caccctaaag cagcgcatcc gcaatgacat
    18601 acttattcct tcggtttaaa ccgcagcaac cggttggcgt tactcactac ggtaatcgac
    18661 gagagcgcca ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac
    18721 aaaataccgg cggcgaccgg aataccgata ctgttgtaga taaaggcacc gagcaggttc
    18781 tgcttcatgt tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg
    18841 ctatggcgca tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccccccatc
    18901 gcaataccga catccgcctg ggccagcgct ggcgcatcgt taatgccgtc gcccaccatt
    18961 gccacctgac gtccttcact ttgcaggcgt ttaatcgctt cggctttacc gtccggcagc
    19021 accccggcga tcacttcatc aatcccggct tctttggcga tcgcattggc ggtggttggg
    19081 ttatccccgg tcaacatcac cagacgatat cccgctttat gcaggcgttg cagcgccgcc
    19141 acgctatcac tacgcaatgg atcgcgtact gccagcaggg ctaccgcttt cccgtcaacc
    19201 gccagcagca caggcgttgc cccttgcgat gcctgagcag taatctccgc ttcgatagct
    19261 ttggtaccaa cctgttgctc atttaacagc gcctgattgc ccagcaataa cgcatgacct
    19321 tcagcttcac cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggcagc
    19381 tgcatatcac ctgctttatc gaggatcgcc cgtgccagcg gatggctgga accttgctcc
    19441 agtgccgccg ccagacgcaa tgcctgcgct tcatcaacat cagcaaacgt tttcactgcg
    19501 acaacctgcg gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt
    19561 gtactggcgc gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc
    19621 ccgacgccgg aaataatcga catcggcgtc gccagcccca gcgcacacgg acaggcgata
    19681 atcagtaccg tggtggcaat caccagggta taaacaattt gcggtgccgg accaaagaag
    19741 taccagattg ccgcactgac cagcgcaatc accaccacca caggtacaaa tacggctgag
    19801 attttatccg ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga
    19861 atgatccgtg acagcgtggt atggctgcca accgcactgg cacgaaacag cacgctaccg
    19921 tcctgtacca ctgtcccggc atggacgctt tcgccttcgc ctttttgctg cgggattggt
    19981 tcgcccgtca gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc
    20041 ggcacgcgat cgccggtcgt caggcgcagc aacatacctg gctgcacttc cgccagaggc
    20101 acgctttttt caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat
    20161 ttctccagcg ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg
    20221 atcagaccga taatcatcgc gctggcttcg taataaagat gtcgcgcttc catcgggaac
    20281 cactgcggcc acaggttgac gctcatcgaa tagagccacg ccacgccagt acccagcgcc
    20341 accagcgtat ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa
    20401 tggccgccgg cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg
    20461 cggttgtcag cggtgaccat catgttatcg ccaatcatcc cccagaccat caccgggata
    20521 cccaccgcca gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc aacggcggtt
    20581 tcctgctggc gctcacggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct
    20641 ttttccaccg cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc
    20701 tccgccaggt ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg
    20761 cgggtgacac agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca
    20821 tcggcggtcg ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat
    20881 gactccgcca gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg
    20941 atggtttcga tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac
    21001 acatccgctt gttccacatc cgggcgctgt tcgagacttt ctttcacgcg tttaacgcag
    21061 tgaccgcagg acaggccgtc cagggtcagg tcgatagttt gtgacataaa acactccttt
    21121 aagacagttt tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa
    21181 attaataaac caggcgagta aaagtccgta aagattaaaa aattggctca atttgcatca
    21241 ggattagaca tttatctctt tgttttcctg aagttaagtt gcgggtgcta agttaaatca
    21301 agatgcctga aaatcggcac cggggtgagg aattacctcc cgcatctata aaaaggagtt
    21361 aacaaaagat gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat
    21421 ttcactcact taacggcgga caaaatgccg actacattcc ctttctggcg aatgtaccta
    21481 gtcaactggc agcagtggct attgtgacct gcgatggcaa catctatcgc gcgggtgata
    21541 gtgattaccg ctttgcgctg gaatccatct cgaaagtctg tacgttagcc cttgcgttag
    21601 aggatgtcgg cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct
    21661 ttaactcagt tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg
    21721 ctggcgccat tgccaccacc agcctgatta acgctgaaaa tgttgaacaa cgctggcagc
    21781 gaattttaca tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca
    21841 accagtcgga acaaacaacc aacttccata accgggccat tgcctggctg ctgtactccg
    21901 ccggatatct ctattgtgat gcaatggaag cctgtgatgt gtacacccgt cagtgctcca
    21961 cgctcctcaa tactgttgaa ctggcaacac ttggcgcgac gctggcggcg gggggtgtga
    22021 atccgctgac gcatgaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa
    22081 tgatgatgga agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg
    22141 gcaaaagcgg tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg
    22201 cgttctcacc accgctggac gaagaaggca acagtgttcg cggtcaaaaa atggtggcat
    22261 cggtcgctaa gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg
    22321 taataacggt aacaaacctc tcggtctatg gaacgtcgtt tccatcggca ttggggcaat
    22381 ggtgggggcg gggatcttcg cgctgctggg gcaggctgca ttgctaatgg aagcctcgac
    22441 ctgggtcgcc tttgcttttg gcggtattgt ggcgatgttt tccggttatg cctatgcgcg
    22501 tctgggggcg agctatccca gcaatggcgg cattatcgac ttctttcgtc gcggattagg
    22561 caacggcgtc ttttcgctgg cgctctcgtt actgtacctg ttgacgctgg cggtgagcat
    22621 cgccatggtc gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca
    22681 ggaggagcac cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa
    22741 ctccttaagc aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat
    22801 gatcctgtta ttgctgatta ttgccggtgt ctggtcgctg caaccagcgc atatttccgt
    22861 ctctgcgccc cccagctccg gtgcgttctt ctcctgtatt gggataactt tccttgccta
    22921 tgcgggcttt ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat
    22981 gccacgggcg tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcact
    23041 ggttttgctt agcgatgtat cggcattaga gttagaaaaa tatgccgata ccgccgtagc
    23101 gcaggctgct tctccgctgc tcgggcatgt gggttatgtg atcgtcgtta tcggcgcttt
    23161 actggcgacg gcttcagcca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa
    23221 catgggcagc gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac
    23281 ctggggcaac attattgtcg tggtgttgat tatgctgatg acggcggcgc taaatttagg
    23341 ctcactcgcc agtgttgcca gcgccacctt tttgatttgc tacctggcgg tgtttgtggt
    23401 ggcgatccgc ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt
    23461 ggtgatgttg ttggtgatcg ttggctttat ctacagtctg tggtcccagg gtagccgtgc
    23521 gttgatatgg attattggct cactcttact cagccttatt gtggcaatgg tcatgaagcg
    23581 caataaaacc gtataacatc tctctgtgcg cagtacttcc tgtattattg tggtggcggt
    23641 cgatattcgc actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagca
    23701 caggaggcgt tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca
    23761 gcaaagccat tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa
    23821 acggttatcg cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac
    23881 ggcaggtggg ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc
    23941 agcggcacag cgccgacgtc aaacgccgca cgctggagaa ggtggcggag atcgaacgac
    24001 acattgagga gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg
    24061 gcgatgacag cgccgattgc ccgattatcg aaaatctctc cggctgctgt catcatcggg
    24121 cagggtgatt aagacgagac tgctcggata tgtagggtta tcccttctat cgcaatgact
    24181 tcaacatgcg tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg
    24241 acgcgcatat gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc
    24301 agctgctgcc cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc
    24361 gacaaccatt tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct
    24421 tgccactccc aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg
    24481 ccgctccaca acaaataacc atttccgccc agcatctcgg ctgccagcag caaaccgccg
    24541 agactgagcc agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt
    24601 tgttggcgct gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg
    24661 cctctaatgg catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt
    24721 cggtgtattt ctgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt
    24781 cagacaccat tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct
    24841 gtaaaaacgc cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt
    24901 cggctttgag gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg
    24961 tacgttccgc tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt
    25021 cgcgaatttc aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac
    25081 gcagcaggcg tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac
    25141 ccaacacggt acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga
    25201 cttcataagc cgcgcgcggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa
    25261 cgttggcgtt atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca
    25321 tattgatctt gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta
    25381 acgttttggt atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt
    25441 tgacgcccgc gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca
    25501 tcggaaaaac ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa
    25561 ttatacctga ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta
    25621 gtaaaaatca gaaaaaggga acagtgatgc aggaaaatag tcctttgctt cagctacaaa
    25681 acgtaggata tctggcgggt gatgcgaaga ttcttaataa catcaatttt tcgctgcgtg
    25741 ctggcgaatt taagttaatt accggtcctt ccggttgtgg caaaagtacg ctgctaaaaa
    25801 tagttgcttc attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca
    25861 gcacactaaa gccagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc
    25921 tgtttggcga tacggtatac gataatctga tctttccctg gcagatccgt aaccagcagc
    25981 ctgacccggc catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga
    26041 cgaaaaatat cgccgagcta tctggtggtg aaaaacaacg catctcactg attcgtaacc
    26101 tgcaatttat gccgaaggtt ttattgctgg atgaaatcac cagtgcgctg gatgaaagta
    26161 ataaacataa cgttaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc
    26221 tgtgggtgac acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac
    26281 cgcatgccgg agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca
    26341 ttggcactgg cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg
    26401 gcgctggaga aagatatttt ctggagcgtc gggcgagcga taattcagct gattattgtc
    26461 ggctatgtgc tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg
    26521 ttatttatct gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa
    26581 gcttttatct catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg
    26641 attctctctg ggtcgattga atttatcccg atgcaggtga tccctatcgc cgggatgatt
    26701 gccggtaacg ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt
    26761 agcgaacagc aacagattca ggagaaactg agtcttggtg cgacgccgaa gcaggcttca
    26821 gcgatattga ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa
    26881 acggttggct tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat
    26941 ccggtgaagg cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc
    27001 ttgtcgacca ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag
    27061 ttggtggtga cgcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg
    27121 gcgttgtggt tcatgtgccg atcaacggaa tatcaataca acaatgcata cagctggcgg
    27181 cgatacttcg acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt
    27241 ttacgcgtct gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc
    27301 agcgcctctt cattgcgccc aacctggtgc agttgcagag ccagttgcgt cgccagtgcg
    27361 gcatcttctg gattctccgc cacctgctgt tgcaactgtt gaatttccgg cgtatcagcc
    27421 gcctgcttca gcagttcgat ttgcgccacc agcccctggt agcgggtgtc ctgatcctgc
    27481 aacggaatgg ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt
    27541 tctgccagca gcaggccgat ctccccgttc tgattcgaca actgccaggc gtctttcagc
    27601 aatggcaggg catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc
    27661 agctcttctt cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc
    27721 ggcccctgga agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg
    27781 cgcagaccaa actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata
    27841 aattgcccgt tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc
    27901 tggctacgtt cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc
    27961 tgttgcaggt tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct
    28021 gttgtcgata ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat
    28081 attttgtcca tcacgcgccc cggcagcagg cgcttgagca ccattactgc ccaggtcacc
    28141 agcgttaccg gatagcgcat cttcggcttc tcgctaataa aagcatggcg tactttgtcc
    28201 accaccgctt ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta
    28261 tcactttgcg tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc
    28321 aggctgactt taattccgct gtggcgcagc tccatgcgca gtgcgtctga ccacgcctcc
    28381 agcgcatatt tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc
    28441 gatgatgtca tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg
    28501 gtgagctggt gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg
    28561 atggtggaaa gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa
    28621 ttatcggtca gggcgatcac ctcgtctgct gcgcgatcaa cactttctgg tgaatccaga
    28681 tcgatcaaca cgccggtaaa tcccatgttg ttcatacgct caacatcatc cggttttcgg
    28741 caacctgcca gcacatgaaa accctggcgt tttaattcga gcgcgctttc taggccaatt
    28801 ccactggaac atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc
    28861 gttgctttat gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat
    28921 aaacggctgg gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag
    28981 gtagacctct tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg
    29041 gtaaatggcg ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg
    29101 cattaacaat ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg
    29161 ctcggtttgc tgtggctgaa aaccacgcaa accgtcattg ccgcccagtt caaccagcac
    29221 ccaacgcggc tgatgctgtt tcagcagagc cggaaggcgc gccagtcctt gttgcgaggt
    29281 gtcgccgctg atgctggcat taactaccga cgttttactc tgccacttat cattcaacaa
    29341 ggcaggccag gccgcgctgg cagacattcg atacccggcg ctcaggctat cacccagaat
    29401 caataacgtg tccgctgcgg cggcacggaa ggttaacagg accaggaaca ggaagggcaa
    29461 atgccagcgg aaaacattgt tgaagttcat catcttaaga agtccgtcgg tcagggggag
    29521 catgaactct ccatcctcac cggagttgag ctggttgtca aacgtggcga gaccatcgca
    29581 ctggtgggcg agtcgggatc gggtaagtca accttgctgg cgatcctcgc cgggctggat
    29641 gacggcagca gtggcgaagt gagtctggtg ggacaaccgc tacataatat ggacgaagaa
    29701 gcgcgggcaa agttgcgcgc gaagcacgtc ggctttgttt ttcagtcatt tatgttaatt
    29761 cctaccctta acgcgctgga aaacgtcgag cttccggcgc tgctgcgcgg tgagagtagc
    29821 gcggaaagtc gtaacggagc gaaagcgttg ctcgaacagt tagggctggg taaacgtctt
    29881 gatcatcttc cggcacagct ttccggcggc gaacagcaac gagtggcgct ggcgcgagcc
    29941 tttaatggtc gacctgatgt gctgtttgcc gacgaaccca ccggcaacct tgaccgccag
    30001 acgggcgata aaattgccga cttgctgttt tccctcaacc gtgagcatgg caccacgttg
    30061 attatggtga cccacgacct gcaactggcg gcacgctgcg accgctgctt acggctggtg
    30121 aacgggcagt tgcaggagga agcatgattg cacgttggtt ctggcgcgaa tggcgttcgc
    30181 cgtcgctatt aattgtctgg ctggcgctaa gcctggcggt ggcctgcgtg ctggcgctgg
    30241 gcaatatcag cgatcgcatg gagaagggct taagccaaca aagccgggag tttatggcgg
    30301 gcgatcgggc gttgcgcagt tcgcgcgaag tgccgcaagc atggctggag gaagcgcaaa
    30361 agcgcggcct gaaagtcggc aagcagctga ctttcgccac catgacattt gcaggcgaca
    30421 caccgcagct ggcgaacgtc aaagcggtgg atgatatcta cccgatgtat ggcgatctgc
    30481 aaactaatcc ccctggcctg aaaccgcagg cgggcagcgt attgctggcc ccacgcctga
    30541 tggcgctgct taacctgaaa acgggcgaca ccattgacgt gggcgatgcc accttgcgga
    30601 ttgccggaga agtgattcag gaaccggatt ccggttttaa ccccttccag atggctccgc
    30661 gtctgatgat gaatctggcg gatgtcgata aaaccggagc cgtgcagccg gggagtcggg
    30721 tcacctggcg ttataaattc ggcggcaacg agaaccagct cgacggctat gagaaatggt
    30781 tgttacccca gcttaaaccc gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc
    30841 tggggcgatc gatggaacgc tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc
    30901 tgctggcagt ggcagcagtg gcggtagcga tgaatcatta ctgtcgcagt cgctacgatc
    30961 tggtggcgat cctcaaaacg ctgggggcag ggcgagcgca actgcgtaag ctaatcgtcg
    31021 gtcagtggtt actggtgctg gcgctttcag ccgttaccgg tggggcgata ggcctgttgt
    31081 tcgaaaacgt gttgatggtg ctgctcaagc ccgttctgcc tgctgcacta ccgccagcca
    31141 gcctctggcc gtggctatgg gcgctgggca ccatgacggt tatctcgctg ctggtggggc
    31201 tacgaccgta ccgcttgttg ctggcaacgc agcctttacg cgtattacgt aatgatgtgg
    31261 tcgcgaacgt ctggccgctg aagttttatc tgccgattgt cagtgtggtg gttgtgctgc
    31321 tgctcgccgg attaatgggt ggcagcatgc tgctttgggc ggtgctggcg ggcgcggtag
    31381 tactggcttt gctgtgcggt gtgctgggct ggatgatgct gaatgtactt cgccgcatga
    31441 cgctgaaatc gctacctctg cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa
    31501 cattaagcca gctttcggca ttttcgctct cctttatgct gctggcgctg ttgctggtat
    31561 tgcgtggcga tctgcttgac cgctggcagc agcagctacc tccagaaagc ccgaactact
    31621 ttttaattaa cattgccaca gaacaggtag aaccgctaaa agcgttcctc gcggaacatc
    31681 agatagtccc ggaatcattt tatccggtag tgcgggcgcg gctgacggcg attaacgata
    31741 agccgacaga aggtaatgaa gatgaggcgc ttaaccgcga actcaatctt acctggcaaa
    31801 atacgcgacc cgatcataat ccgattgtcg ccggtaactg gccgccaaaa gccgatgaag
    31861 tgtcgatgga agaggggctg gcgaaacgct taaacgttgc cctcggtgat accgtgactt
    31921 ttatgggcga tacccaggag ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg
    31981 aaagtctgcg gcctaatttc tattttattt tccctgaagg ggcattagac gggcaaccgc
    32041 agagctggct taccagtttc cgctgggaga atggcaacgg catgttgaca caactcaacc
    32101 gccagttccc gaccattagt ctgttagata ttggcgcgat tttaaaacag gtcggtcagg
    32161 tgctggagca ggtaagtcgg gcgctggaag tgatggtggt actggtcacc gcctgcggta
    32221 tgttgctgtt gctggctcag gtgcaggtgg gaatgcgtca gcgtcatcag gagctggtgg
    32281 tgtggcgcac actcggtgcg gggaaaaagc tgctgcgcac gacattgtgg tgtgagttcg
    32341 ccatgcttgg gtttgtttct ggcctggtag ccgcaattgg tgcggaaacg gcgctggcag
    32401 tgttgcaggc gaaagtgttt gattttccgt gggagccaga ctggcgattg tggattgttc
    32461 tgccgtgcag cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg
    32521 ttaagggtaa ggcgctgttc aggcagtttg cggggtgatg agagtaaata aggggcgttc
    32581 tggttgaatg gaacgccttg tttaaattaa ttctacaata tgtttcattg atgatgttat
    32641 tgaattggtg atttcctatc tttctattgc gatattaatc tgaatcggtg gggtttaata
    32701 attttaaata tagccctaca aaaataactc agactaataa aatcataaat catatgcgtt
    32761 gaatggatat tatccatata gtgaatttgt tgatgatgaa ttcatctgtg c
//
LOCUS       DAZGSP010000051        32775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZGSP010000051 DAZGSP010000000
VERSION     DAZGSP010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.051"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..612
                     /locus_tag="ACHK74_002653"
     CDS             <1..612
                     /locus_tag="ACHK74_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP0221445.1"
                     /translation="SRRRNAIYPAWYWLTVLDVLRYTGMRFNQLKHIRVRDVCPDESI
                     IDLKLEGSKTHREWGVPLVAPLKVSMEKLLERARQCGAGDDDYLFDVCRFSEPISADR
                     YQYCPVRAHQTVRSFFRRLSRECGFPVSPHRFRHTLATRLMKYPERNLHLVKSILGHR
                     SLTTTMGYVDVCMESAAQTLEDALSLYTDERTVSKICKGGNKS"
     gene            complement(808..883)
                     /locus_tag="ACHK74_002654"
     tRNA            complement(808..883)
                     /locus_tag="ACHK74_002654"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(848..850),aa:Phe,seq:gaa)
     gene            complement(989..1696)
                     /gene="yqgA"
                     /locus_tag="ACHK74_002655"
     CDS             complement(989..1696)
                     /gene="yqgA"
                     /locus_tag="ACHK74_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFP0221446.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPTHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1849..1932
                     /gene="yqgH"
                     /locus_tag="ACHK74_002656"
     CDS             1849..1932
                     /gene="yqgH"
                     /locus_tag="ACHK74_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFP0221447.1"
                     /translation="MHATSLHSLAYRRFARNVSLSMLLLMR"
     gene            2094..4229
                     /gene="speC"
                     /locus_tag="ACHK74_002657"
     CDS             2094..4229
                     /gene="speC"
                     /locus_tag="ACHK74_002657"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFP0221448.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMEDSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4279..5535)
                     /gene="nupG"
                     /locus_tag="ACHK74_002658"
     CDS             complement(4279..5535)
                     /gene="nupG"
                     /locus_tag="ACHK74_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFP0221449.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAVLVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5737..6816)
                     /gene="mltC"
                     /locus_tag="ACHK74_002659"
     CDS             complement(5737..6816)
                     /gene="mltC"
                     /locus_tag="ACHK74_002659"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFP0221450.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6881..7156)
                     /gene="yggX"
                     /locus_tag="ACHK74_002660"
     CDS             complement(6881..7156)
                     /gene="yggX"
                     /locus_tag="ACHK74_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFP0221451.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7184..8236)
                     /gene="mutY"
                     /locus_tag="ACHK74_002661"
     CDS             complement(7184..8236)
                     /gene="mutY"
                     /locus_tag="ACHK74_002661"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFP0221452.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8397..9116
                     /gene="trmB"
                     /locus_tag="ACHK74_002662"
     CDS             8397..9116
                     /gene="trmB"
                     /locus_tag="ACHK74_002662"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFP0221453.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9116..9442
                     /gene="yggL"
                     /locus_tag="ACHK74_002663"
     CDS             9116..9442
                     /gene="yggL"
                     /locus_tag="ACHK74_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFP0221454.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            9626..10344
                     /gene="yggN"
                     /locus_tag="ACHK74_002664"
                     /pseudo
     CDS             9626..10344
                     /gene="yggN"
                     /locus_tag="ACHK74_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
     gene            10520..11566
                     /gene="ansB"
                     /locus_tag="ACHK74_002665"
     CDS             10520..11566
                     /gene="ansB"
                     /locus_tag="ACHK74_002665"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFP0221455.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDISKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            11683..12690
                     /gene="yggM"
                     /locus_tag="ACHK74_002666"
     CDS             11683..12690
                     /gene="yggM"
                     /locus_tag="ACHK74_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFP0221456.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSNDDLYTWVGQLADYELLEQTWTKDKAVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13019..14155)
                     /gene="hemW"
                     /locus_tag="ACHK74_002667"
     CDS             complement(13019..14155)
                     /gene="hemW"
                     /locus_tag="ACHK74_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFP0221457.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMHTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(14148..14741)
                     /gene="rdgB"
                     /locus_tag="ACHK74_002668"
     CDS             complement(14148..14741)
                     /gene="rdgB"
                     /locus_tag="ACHK74_002668"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFP0221458.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(14749..15039)
                     /gene="yggU"
                     /locus_tag="ACHK74_002669"
     CDS             complement(14749..15039)
                     /gene="yggU"
                     /locus_tag="ACHK74_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFP0221459.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(15036..15602)
                     /gene="yggT"
                     /locus_tag="ACHK74_002670"
     CDS             complement(15036..15602)
                     /gene="yggT"
                     /locus_tag="ACHK74_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFP0221460.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(15620..16324)
                     /gene="yggS"
                     /locus_tag="ACHK74_002671"
     CDS             complement(15620..16324)
                     /gene="yggS"
                     /locus_tag="ACHK74_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFP0221461.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            16342..17322
                     /gene="yggR"
                     /locus_tag="ACHK74_002672"
     CDS             16342..17322
                     /gene="yggR"
                     /locus_tag="ACHK74_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFP0221462.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            complement(17498..17914)
                     /gene="ruvX"
                     /locus_tag="ACHK74_002673"
     CDS             complement(17498..17914)
                     /gene="ruvX"
                     /locus_tag="ACHK74_002673"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFP0221463.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(17914..18477)
                     /gene="yqgE"
                     /locus_tag="ACHK74_002674"
     CDS             complement(17914..18477)
                     /gene="yqgE"
                     /locus_tag="ACHK74_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFP0221464.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(18586..19536)
                     /gene="gshB"
                     /locus_tag="ACHK74_002675"
     CDS             complement(18586..19536)
                     /gene="gshB"
                     /locus_tag="ACHK74_002675"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFP0221465.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(19549..20280)
                     /gene="rsmE"
                     /locus_tag="ACHK74_002676"
     CDS             complement(19549..20280)
                     /gene="rsmE"
                     /locus_tag="ACHK74_002676"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFP0221466.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(20360..21067)
                     /gene="endA"
                     /locus_tag="ACHK74_002677"
     CDS             complement(20360..21067)
                     /gene="endA"
                     /locus_tag="ACHK74_002677"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFP0221467.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAAGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(21162..21659)
                     /gene="yggI"
                     /locus_tag="ACHK74_002678"
     CDS             complement(21162..21659)
                     /gene="yggI"
                     /locus_tag="ACHK74_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFP0221468.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(21736..23129)
                     /gene="galP"
                     /locus_tag="ACHK74_002679"
                     /pseudo
     CDS             complement(21736..23129)
                     /gene="galP"
                     /locus_tag="ACHK74_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
     gene            complement(23565..24719)
                     /gene="metK"
                     /locus_tag="ACHK74_002680"
     CDS             complement(23565..24719)
                     /gene="metK"
                     /locus_tag="ACHK74_002680"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFP0221469.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLREAAGLK"
     gene            24775..25026
                     /gene="yqgD"
                     /locus_tag="ACHK74_002681"
     CDS             24775..25026
                     /gene="yqgD"
                     /locus_tag="ACHK74_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFP0221470.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            25137..25280
                     /gene="yqgG"
                     /locus_tag="ACHK74_002682"
     CDS             25137..25280
                     /gene="yqgG"
                     /locus_tag="ACHK74_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFP0221471.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            25374..25505
                     /gene="yqgB"
                     /locus_tag="ACHK74_002683"
     CDS             25374..25505
                     /gene="yqgB"
                     /locus_tag="ACHK74_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFP0221472.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            25514..27490
                     /gene="speA"
                     /locus_tag="ACHK74_002684"
     CDS             25514..27490
                     /gene="speA"
                     /locus_tag="ACHK74_002684"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFP0221473.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INTAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            27636..28367
                     /locus_tag="ACHK74_002685"
     CDS             27636..28367
                     /locus_tag="ACHK74_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221474.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGMDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            28503..29423
                     /gene="speB"
                     /locus_tag="ACHK74_002686"
     CDS             28503..29423
                     /gene="speB"
                     /locus_tag="ACHK74_002686"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFP0221475.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDIDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(29629..30387)
                     /gene="loiP"
                     /locus_tag="ACHK74_002687"
                     /gene_synonym="yggG"
     CDS             complement(29629..30387)
                     /gene="loiP"
                     /locus_tag="ACHK74_002687"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFP0221476.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKALSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            30665..32656
                     /gene="tkt"
                     /locus_tag="ACHK74_002688"
     CDS             30665..32656
                     /gene="tkt"
                     /locus_tag="ACHK74_002688"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP0221477.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTNFGESAPAELLFEEFGFTVDNVVAKAKELL"
BASE COUNT         7945 a         8301 c         8670 g         7859 t
ORIGIN      
        1 agtcgtcgac gcaatgctat ataccctgcc tggtactggc tgacagttct ggatgttctg
       61 cgttataccg gaatgcgctt taatcaactg aagcatatcc gtgtgaggga tgtctgtccg
      121 gatgaaagca taattgatct taagctggag ggaagtaaaa ctcacaggga atggggcgtt
      181 cctctggtgg ctcccctgaa ggtatcgatg gaaaaactgc tggagcgggc ccgtcagtgc
      241 ggggcaggtg atgatgacta tctttttgac gtctgtcgtt tcagtgagcc gatctctgcc
      301 gacagatatc agtactgtcc cgttcgggcg catcagaccg tacgttcatt tttccggcga
      361 ctttcgcgag aatgtggttt tcctgtttca ccacatcgtt tcagacatac gctggcaacc
      421 agactgatga agtatcctga acgtaattta catctggtca aatccatact gggacacagg
      481 agtcttacaa cgaccatggg gtatgttgat gtctgtatgg agtctgcagc gcagacactg
      541 gaagatgccc tgagtttgta tacagatgag cgcacagtga gcaagatttg caaagggggg
      601 aataaatctt gacaggctga atcagcaatg taagcataca gtgttaacga gaacagggcg
      661 tctctgcaaa agacaccctg tttgaggata cgataccgaa ctcaatcagt gtaaccacaa
      721 cttacacaaa cggagaccct ttgatgccgc atcctgtatt aaccgcccgt caaggatctc
      781 atcccttaac cagcgtgttc tggtatatgg tgcccggagg cggaatcgaa ccaccgacac
      841 ggggattttc aatcccctgc tctaccgact gagctatccg ggcaacgggg cgcattaaac
      901 ctgattcgcc tcgtctcgtc aatcaaattt cttcaattta ctgcagactg cacaatctat
      961 catcactttg ccgctattgc acgcattgtc aggcaaacca ggcggtccag gcggcggaaa
     1021 gcggcattgc cagaaggagt gctggaagca tgttgaccac cgggaacatt ttaatgccac
     1081 agatgcgtaa tccggttgcc agcagcaata aaccgcctac ggcgctgaag tctgccatca
     1141 tcgacggtgt ggtcagcggt aatatcagcg cggcagccca cgccagcgtt aactggatga
     1201 tcagtaatgg gatactaatt accgacaccg caatacccag cgagcaggcg aagatcatcg
     1261 ccgtaaagaa atcaagaaat gacttggcga ttaaaatact cggatcgccg gtcatccctt
     1321 cgttcatcgc cccgaagatc ccggtgccgc tggcgcaaaa caggacaata atcgcgacat
     1381 aattctgaat aaaagattca tgcgttggct tcttacgtga gtgacgaaac agattttgtg
     1441 ctttggcgac tgctgtattg acacctttct ccagcaaaca aatttcgcca attagagcgc
     1501 caagtagcgt agctaaaacc atcgctggaa ggttggcgca tttcacaacc agtaaaatac
     1561 caatccccag cgatgccaga ccaaaaatag atgtcatgga gacgcggata cgttccggta
     1621 agcgttggct gagcagtgcg ccgagaacgc cacccagtaa gactgcacta gcgttgataa
     1681 aagggccgat gaccacaaga gttcctgtta gctgtattgt ttgattttct tattatgaac
     1741 tttttgcctt gttgatggct tgtctggtcg caaagtcctg gcttgcacgc tttagcgaaa
     1801 ggtgccatga ttgcgcgaat tttctcctct ctgtacggag tttgcccgat gcacgccacc
     1861 tccttacatt ctctcgctta tcgccgtttc gcgcgaaacg tttccctttc tatgttactg
     1921 ctcatgcggt gaagttaacg cacgctcact gcaggacaac agtaaaatca gagcgtttct
     1981 gcttttactg atgtctggcg gtcggagctg gtgaccagtt tgacccatat ctcatggggc
     2041 agggttttcc accttgtccg gtattcttac ttccccgaaa cgggtttgcg cttatgaaat
     2101 caatgaatat tgccgccagt agtgaactgg tatcccgact ttcttctcat cgtcgcgtgg
     2161 tggcgttggg agatactgat tttacggacg tcgcggcagt cgtcattacc gctgcggata
     2221 gtcgcagtgg cattcttgcg ttgcttaagc gcaccggttt tcatctaccg gtgtttttgt
     2281 attccgaaca tgctgttgaa ttacctgcgg gcgttacggc ggtaatcaac ggcaacgagc
     2341 agcagtggct ggagctggaa tccgcagcct gtcagtatga agagaatttg ctgccaccgt
     2401 tttatgacac gctgacgcag tacgttgaga tgggcaacag cacctttgct tgccctggac
     2461 atcaacatgg tgcgtttttt aaaaagcatc ctgccggacg ccatttttac gatttctttg
     2521 gtgagaacgt ctttcgcgcc gatatgtgta acgctgacgt aaaattgggc gatctgctta
     2581 ttcatgaagg atcggcgaaa gatgcgcaga aattcgcagc caaagtcttt catgccgata
     2641 aaacctattt tgtgctgaac ggcacatcgg cagcgaataa agtggtgacg aatgcgctgt
     2701 taacgcgtgg cgatctggtg ctcttcgacc gtaacaacca taagtcgaat catcacggcg
     2761 cgctgattca ggcgggggcg acgccggtct atctggaagc ttcacgcaac ccgtttggtt
     2821 tcattggcgg tattgatgcg cactgtttta atgaagagta tctgcgccag caaattcgcg
     2881 acgttgcgcc agaaaaagct gagttgccgc gcccgtttcg cctggcgatt attcagctgg
     2941 gaacctatga cggcactgtc tataacgccc gtcaggtgat cgataccgtt gggcatctgt
     3001 gtgattacat tctgtttgat tccgcgtggg tcggttatga acaatttatc ccgatgatgg
     3061 aggatagctc gccgctgctg ttagaactta acgaaaacga tccggggatc tttgtgactc
     3121 agtcggtgca caaacagcag gcgggattct cacagacgtc gcagatccat aaaaaagata
     3181 accatatccg cggacaggcg cgtttttgcc cgcataagcg gttgaataat gcctttatgc
     3241 tccatgcttc taccagcccg ttctacccgc tgtttgccgc gctggacgtt aacgctaaaa
     3301 ttcatgaagg agagagtggg cgtcggctgt gggctgagtg cgttgagttg ggtattgagt
     3361 cgcgcaaggc tattcttgcg cgctgtaagc tgttccgccc gtttatcccg cccgttgttg
     3421 atggcaaatt gtggcaggat tatccgacat ctgtgttagc cagcgaccgc cgttttttca
     3481 gttttgagcc gggggcgaag tggcacggct ttgaaggata tgccgcggat cagtattttg
     3541 ttgatccgtg caagctgtta ctcactacac caggtatcaa tgccgaaact ggcgaatata
     3601 gcgactttgg cgttccggcg acgattctgg cgcactatct gcgtgagaac ggcattgtgc
     3661 cggagaagtg cgatctcaac tccattctgt tcttattaac tccggcggaa agccacgaga
     3721 agctggcaca actggtggcg atgctggcgc aatttgaaca gcatattgag gatgactcgc
     3781 cgctggctga ggtgttgccg agcgtttata acaaatatcc ggtgcgctat cgcgactaca
     3841 ctttgcgcca gttgtgtcag gagatgcacg atctgtatgt cagtttcgac gtcaaagacc
     3901 tacaaaaagc gatgttccgc cagcagagtt tcccgtcagt ggtgatgaac ccccaggatg
     3961 cgcatagcgc ttatattcgc ggtgaagtgg agttggtgcg gattcgtgat gccgaagggc
     4021 gaattgcggc agaaggggcg ttgccttatc cacctggcgt gctttgcgtg gtacccgggg
     4081 aagtctgggg tggggcggtt caacgttatt tccttgcact ggaagaaggg gtgaatttgt
     4141 tgccgggatt ttcgccggag ctgcaaggtg tttatagcga aaccgatgcg gatggcatga
     4201 aacggttgta cggttatgtg ttgaagtaag aataaaaaaa acgggtcgcc ttctggcgac
     4261 ccgattttct ttgcgtaatt agtggctaac cgtctgtgta cctgtcggga cacgaacgtg
     4321 tttatatttg aacatcgcca tgaacgcgaa ggccagaacc acggagtaac cagcgaaaat
     4381 caaccatacg gtctgccagt cggtaatgcc gttttgggtg tacatctcaa caactttacc
     4441 gctcacgatg ccgccgagga tacagccgaa gccgttagtc atcatcagga acatcccttg
     4501 tgcactggcg cgaattgccg ggctaacttc tttttcgaca aacaccgaac cagagatgtt
     4561 gaagaagtcg aatgcgcaac cgtaaacgat catcgacagt accagcagta cagtaccgaa
     4621 cggagtcggg tcgccgtaag caaacagcgc aaaacgcagg atccacgcca caatactgat
     4681 catcattacg ttcttaatac cgtagcggct taagaagaac gggatggtca gaatgaacag
     4741 ggtttcagag atctgcgaaa tcgacatgat gattgacgca tgctgcacaa taaagctgct
     4801 ggcaaacatc ggatctttgt cgaagctgtg caggaaggta ttaccgaaca tgttggtaat
     4861 ctgcagttcc gcgcccagca gcattgagaa gatgaagaag attgccatac gcttgttttt
     4921 aaacagcgcg aatgcatcga ggcccagcag ggttgtccag ctctgattcg cttgctgttt
     4981 agcaaccgga atatgcggca gagtcagggt aaacagaacc agaacggcgg aaagagctgc
     5041 gccaatatac agctgcatgt ggcttaattc gaagccagac aggctcacca cccacattgc
     5101 catgataaag ccgatggtgc cccagatacg gattggcggg aagtcagtaa cgatatccat
     5161 cccggcattt tgcaggcgat agtaagagat ggtgttgatt aacccaagcg ttggcatata
     5221 agcaaacgag ttaatcaata tcacaaggaa catcgcctcc ggcgtcgtga cctgtgccgc
     5281 catgaacagc gtgatagcgc caatggtgtg gcaaatggca tatacccatt tcgcacttaa
     5341 ccatttgtcg gccacaatcc ccagcagcgc aggcataaag accgcggcga tacccagtga
     5401 gctataaact gcgccaatag aagcaccgtc aaacttcagg gtaacaaaca tataggagcc
     5461 gagggtcgtc agccaacttc cccacagaca gaactgcaga aaagagagga ttttcagctg
     5521 cagcttaaga ttcatgttaa tttcctcaca tcgtgatgcg gatgagtgtt tgaaaaagca
     5581 catttgaccg tgcgagatga tgggattcta tcaacttcgc ggactggttt tttgttacct
     5641 gtggcaaata attgcaaaca ttcccaattt gcaaaacgaa attgtgatat ggataacatt
     5701 tttgcccctg agcatcgtca ggggcggtta atggaattat cggcggcggt aggatttttg
     5761 cgcggtattc actttataaa gataacggcg agattccgca gagggatggc gggtcgtcag
     5821 cgtctgataa acatcgcccg gcgtcatggt gttaataata ttggccgcct gaatcttatc
     5881 attcgaaaag actcgcagca cgctgcctgc gccgccgtta taggcggtga tgacggcata
     5941 acgccgtgac gttgggttat caattccgcc gagataaaca ttgttcagca tcgccagata
     6001 cgcggtgccg gtatcaatat tgctggcagg atcaaacaag aaactgcggc tcggcgtgcc
     6061 ggatttcccc tgcgagcgga acacatcttt cccggcagta tgttgcacca cctgcattaa
     6121 ccccagcgca tcggaacggc tgaccgcata cgggttaaag gaagattcag tctgcataat
     6181 tgccagaatc agcgactcat caacgccata tttccgtgac gcctggcgga ccatgccgag
     6241 atatttgtgc gcacgtttat caaggtggtt cggcaccatg ttaatggtga cgctgtagat
     6301 gatacgcagc ccgttgctgc ggcttttcag acggtttttc agcagataat ccgcgaagtt
     6361 gcttgcgcga ccttcccagc gaatcggctg cccggtgttg tccaccacct gaccgtaaag
     6421 gaaaggttct ttcgaaattg taatatcatc aacgtcggaa tagagatcga ccgaactcgg
     6481 atcgtcaccc atcagcaacg ttttgataat tgcccggcgc aaatgcgcgg caggttctgt
     6541 cccggcgatg gtttcgatag taatcgtacc gtcatcgaag ttgatgtggc tgcgggtctg
     6601 atattgatcg gtgtatttca cgtagtcctt aggaccagcg atcaccacct ctttgaagcc
     6661 ccagatgttc tcaatattgt gggcaaattg ccccatcaga atatcaaaac cgttggtgtc
     6721 tttgacccag gcttcgttat aggtatcgcc ttttttggtc gtcgaacagg agatgagcaa
     6781 cggcgcaatc aaagccagcg cgagatattt tttcatcatt ccgggtgcgt gttgtgtttg
     6841 tttttatgca agctgccgga ggcgtgctcc ggcactgttt ttatttttta tcttccggcg
     6901 tatagccctc gatatgcacc tctttaccct cgaacaggaa gttgaccatc tcctgctcca
     6961 gcagcttgcg gtgctcggca ttcatcatgt tgagtttctt ttcattaatc agcatggttt
     7021 gtttgtgctg ccactgcgcc caggcttctt tggagatctc gttatagatg cgttttccca
     7081 gttcgccggg gtacagctga aaatcctgac cttctgcttc acgttgcagg aaagtacaaa
     7141 aaatcgttct gctcataaat catcctcttt atcgactcac gcgctaaacc ggcgcgccag
     7201 tgcgtaactg ctgtaacaaa cgctccacgg gagccgccag gccaactgac ggcggttgcg
     7261 ctaagttata ccagagcgca ttgccttcat ccatgcagcc ggtgaatgac gacacgggaa
     7321 gccacatagg cacaatatct aagtggaaat ggctgaaggt atggcgaaac gcggtcagct
     7381 gcgtcaggtt atcggcagca atctgccgtt gcgccagcca ctgccgcaaa ctttcttcgt
     7441 cggcaaactg cgggaaacag tataaaccgc cccacaatcc gctcggcgga cgctgcgcca
     7501 gtaatacttc atcttcgtgc tgtaacagca aaaagtagcc ggtgcgctcc ggcagagtct
     7561 gtttcggttt tttgcccgga taaagcgacc agctattgtt ggtggcggca atacatccgt
     7621 tttgtagcgg acagagcgaa catttcggct tcgaacgcgt gcaaatcatc gcgcccaaat
     7681 ccatcatcgc ctgattaaac cgttccacgc cgaccgcggg cgtcacctgc tcgcttaaac
     7741 tccataattt attctcgacc tcttttttcc caggccagcc gcttacagca tagcagcgcg
     7801 ccagcacccg tttgacgtta ccgtcgagaa tcggaaagtg cttacccaga gaaagcgaga
     7861 gaatcgcgcc tgcggtggaa cgcccgacgc ccggcagtgc tgcaacttcc tcaaaggttt
     7921 ccgggaattt accgccgtgt aaggtcgcca cttgttgtgc cgctttatgc agattgcgcg
     7981 cgcgggcgta atagccaagc ccggtccaca agtggagaac ttcgtcgagc ggcgcattgg
     8041 cgagatcggt caccgtcggg aagcgcgcca taaagcgttc aaaatagggg ataacggtcg
     8101 caacctgagt ttgttgcaac atcacttctg agagccatac tttgtagggc gtcttgtcaa
     8161 tttgccaggg cagagttttt cgcccgtatt tatcgtacca gtccagaacc tgggctgaaa
     8221 attgcgacgc ttgcatggtc accgaattca ctgttgttgg gggcaagatt gcagcacagc
     8281 gacggcaggg tgtaaaccgg aactttccgc agccacggcc ttatcatgct tgcatccggc
     8341 aactaacttt ggataatgcc cgttttcaga acactttcac aagcgactaa atctttatga
     8401 aaaacgacgt catttcaccg gaatttgatg aaaacggccg cccactgcgc cgtatccgta
     8461 gttttgtgcg ccgccagggg cgactgacca aaggccagga acatgcgctg gaaaactact
     8521 ggccggtgat gggcgttgag ttcagcgaag atatgctgga tttccccgcg ctttttggcc
     8581 gtgaagcgcc ggtgacgctt gagattggtt ttggcatggg ggcgtcgctg gtggcaatgg
     8641 ctaaagatcg ccccgagcag gacttcctcg gcattgaagt gcattcaccg ggcgttggtg
     8701 cgtgcctggc ttctgcgcat gaagagggct taagcaacct gcgcgtgatg tgtcacgatg
     8761 cggttgaagt gctgcataaa atgattcctg acaattcatt gcgcatggtg cagctctttt
     8821 tccctgaccc gtggcacaaa gcgcgccata ataaacgccg tatcgttcag gtgccgtttg
     8881 ccgaactggt aaaaagcaaa ctgcagctgg ggggcgtatt ccatatggcg accgactggg
     8941 aaccttatgc ggaacatatg cttgaagtga tgtcttctat tgacggttat aaaaacctgt
     9001 cagagagcaa tgattacgta ccgcgtccgg catcacgtcc ggtgacgaaa tttgaacaac
     9061 gtggtcatcg tcttggtcac ggagtatggg acttaatgtt cgagagggtg aaataatggc
     9121 aaagaaccgt agccgtcgtc tgcgtaaaaa aatgcacatc gacgaattcc aggaattagg
     9181 attttcggtg gcatggcgat tcccggaagg tacatcggaa gaacagattg ataaaactgt
     9241 tgatgatttt attaacgagg ttatcgaacc gaacaaactg gcctttgacg gcagcggtta
     9301 tctggcctgg gaaggtctga tctgcatgca ggaaatcggc aaatgcaccg aagaacatca
     9361 ggcgattgtg cgtaagtggc tggaagagcg caaactggat gaggtacgca ccagcgaact
     9421 tttcgacgtt tggtgggact aagaaagcat acgggcgatg acaaatgcaa aactgcctga
     9481 tgcgctacgc ttatcatgcc tacaaagatg cacgatcgag taggcgggat aaggcgttta
     9541 cgccgcatcc ggcatggaaa acgcgtactt tgttatcaat ctggggccag caaatgctgg
     9601 cctgatttgt tcttgaggga agactatgat gcgcaaaatg ctgctggcgg cagcactttc
     9661 agtgacggca atgaccgctc acgccgacta ccagtgcagc gtcacgccgc gtgacgatgt
     9721 gattgtcagc ccgcaaaccg tgcaggtgaa gggcgaaaac ggcaatctgg tgatcacgcc
     9781 agacggcaac gtgatgtata acggtaagca atattccctg aatgccgccc agcgcgagca
     9841 ggcgaaggat tatcaggctg aactacgtag cacgctgccg tggattgatg aaggcgcgaa
     9901 aagccgcgtc gaaaaagccc gtattgctct ggataaaatt atcgttcagg agatgggcga
     9961 aagcagcaaa atgcgcagcc gtctgaccaa acttgatgcg cagctgaaag agcagatgaa
    10021 ccgcattatc gaaacgcgca gcgatggcct gacgttccac tataaagcca ttgatcaggt
    10081 tcgcgccgaa ggccagcaat tagtgaatca ggcaatgggg ggaattttac aggacagcat
    10141 taatgaaatg ggcgcgaaag cggtgctgaa aagcggcggt aacccattac agaatgtgct
    10201 gggaagcctg gggggctgca atcctcaatc caaaccgagt ggaaaaagca ggaaaaagat
    10261 ttccagcagt ttggcaaaga tgtttgtagc cgcgttgtga ctctggaaga tagccgcaaa
    10321 gccctggtcg ggaatttaaa ataatcctct attttaagac ggcataatac ttttttatgc
    10381 cgtttaattc ttcgttttgt tacctgcctc taactttgta gatctccaaa atatattcac
    10441 gttgtaaatt gtttaacgtc aaatttccca tacaaagcta agggataatg cgtagcgttc
    10501 acgtaactgg aggaatgaaa tggagttttt caaaaagacg gcacttgccg cactggttat
    10561 gggttttagt ggtgcagcat tggcattacc caatatcacc attttagcaa ccggcgggac
    10621 cattgccggt ggtggtgact ccgcaaccaa atctaactac acagcgggta aagttggcgt
    10681 agaaaatctg gttaatgcgg tgccgcaact gaaggacatt gcgaacgtta aaggcgagca
    10741 ggtagtgaat atcggctccc aggacatgaa cgataatgtc tggctgacac tggcgaaaaa
    10801 aattaacacc gactgcgata agaccgacgg cttcgtcatt acccacggta ccgacacgat
    10861 ggaagaaact gcttacttcc tcgacctgac ggtgaaatgc gacaaaccgg tggtgatggt
    10921 cggcgcaatg cgtccgtcca cgtctatgag cgcagacggt ccattcaacc tgtataacgc
    10981 ggtagtaact gcggcagata aagcctccgc caaccgtggc gtgctggtgg tgatgaacga
    11041 caccgtgctg gatgggcggg atgtcaccaa aaccaacacc accgacgtag caaccttcaa
    11101 gtctgttaac tacggtcctc tgggttacat tcacaacggt aagattgact accaacgtac
    11161 cccggcacgt aagcacacca gcgatacgcc attcgatatc tctaagctga atgagctgcc
    11221 gaaagtcggc atcgtttata actacgctaa cgcatccgat cttccggcta aagcactggt
    11281 agatgcgggc tatgatggca tcgttagcgc tggtgtgggt aacggcaacc tgtataaaac
    11341 cgtatttgac accctggcaa ccgccgcgaa aaacggcact gcggtagtgc gttcttcccg
    11401 cgtaccaacg ggcgcaacca ctcaggatgc cgaagtggat gatgcgaaat acggcttcgt
    11461 cgcctctggc acgctgaacc cgcaaaaagc gcgcgttctg ctgcaactgg ctctgacgca
    11521 aaccaaagat ccgcagcaga tccagcagat cttcaatcag tactaatcgc ctcgccccgg
    11581 tatcgtgccg gggctttttc acttcagact tacgtccatt gccaattttc attaccctaa
    11641 tgataatcac cggaataaat tattccgcgc gagggttttc gggtgaaaaa gcaatggatt
    11701 gttggcacgg cgctgcttat gttgatgact ggtaatgcct gggcagatgg cgaaccgcca
    11761 actgaaaata tcttaaaaga ccaattcaaa aagcagtatc acggcattct caagcttgat
    11821 gctatcacct taaaaaatct tgatgctaag ggtaatcagg ccacctggtc agcggaaggc
    11881 gatgtctctt ccaatgacga tctctatacc tgggtcggtc agttggcaga ttacgaactg
    11941 ctcgaacaga cctggacgaa agataaagcg gtaaaattct cggcgatgtt aaccagtaaa
    12001 ggaacgcccg cgtctggctg gtcggtgaac ttttactctt ttcaggcggc agccagcgat
    12061 cgtgggcggg tggtcgacga tatcaaaacg aataataaat atctgatcgt gaatagcgaa
    12121 gatttcaatt atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt
    12181 cctgcgctgg aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca
    12241 gcggatgcat attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agacgccttt
    12301 aaaaaaattc accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg
    12361 gttaaatatg acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa
    12421 gagtgttatg aagtgccgat tcagcagaag cgagatttcg atatcaacga acaacggcga
    12481 cagacttttc tgcaatcaca aaaacttagc cgtaaattgc aggatgactg ggtaacgctc
    12541 gaaaaagggc aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg
    12601 attttgatga agattgacga tatcaaccag gctaatgaac gctggaaaaa agataccgaa
    12661 caattgcggc gcaatggggt gattaagtaa gtttgatata gtgcacgatt tgtagcagga
    12721 tgcggcgttg accccgcatc cggcaatcaa cgtctgatgc gacgcttgcg cgtcttatca
    12781 gacctacaac cgctgccaca tgtcggctga ggcgctcacg ccgcatccgg caatcaacgt
    12841 ctgatgcgat gcttgcgcgt cttatcagac ctacaaccgc tgccacatgt cggctgaggc
    12901 gctcacgccg catccggcaa tcaacgtctg atgcgacgct tgcgcgtctt atcagaccta
    12961 caaccgttgc cacatgtagg ctggataagg cgttcacgcc gcatccggca atacaagttt
    13021 actcagccag aaaaagctcc agcagcgaat ttaaaaacag cttcccatgt tccgtaatct
    13081 gccagtaatc cgcacattcg gtgagataac cctgggcaat tgcctcgtct aactgtgggc
    13141 gaatcacatc ttcgcaaagc ccggtatacg cactaaactc tgcgcgtggc gcggcctcca
    13201 gcaagcgaaa gcggttcata aagaactcaa acggcttatc tgcagcttcg acatcacgct
    13261 ggctttccag ataccttccc tgcataaaac cacgcggatg acgcgttttg gtggtacgca
    13321 gaatgcgccc atccgggaag gtcactttgc cgtgcgcgcc gcagccaata ccgatgtagt
    13381 caccaaagcg ccagtagttg agattgtgct ggcactgata actgggtttg gcgtaagcgg
    13441 aagtttcata ttgctgataa cccgctgcgg ttaataactg atgcccctgt tcgaatatat
    13501 cccacagcgc gtcgtcgtcc ggcagtaccg gcgggcgtga accaaacagc gtattaggtt
    13561 cgatggtcag ttgataccag gaaagatgcg gcggattcag ttcaatggcc tggcgtagat
    13621 cgccaagcgc ctcttccagt gattgatccg gtagtccatg catcaaatcg aggttaaagc
    13681 tacgcagccc cagcccgctc gccagctttg ccgcgcgttt cgcttcttgc gggccatgaa
    13741 tacgcccaag tcgtttcagc ttttcttcgc taaaactctg cacaccaata gagatgcggt
    13801 tcacaccagc acgctgataa tcgacaaagc gatcggcttc taccgtacca gggttcgctt
    13861 ccatagtaat ttctgcatcc gctgccagcg gcaaacgcgc acgcacgccg tccagcagcg
    13921 tgtgcatcgc cgggccggaa agcaggctcg gcgtaccacc gccaataaag attgtcttta
    13981 cttcacggcc ctgagcgtaa gccacatcgt tgtccagatc gttaagcaga tgctgaacat
    14041 aatcgtcgtg cggcacttct cctttcaacg cgtgagagtt gaaatcgcag tacgggcatt
    14101 tctgcacgca ccacgggatg tgaatgtaga gactcagcgg cggtaattta accattacgt
    14161 aaagcgtcca gcagcagttt caatgcctga ccacggtggg aaatggcgct cttttcttcg
    14221 cgggtcagtt cggcagcggt tttcccttcg gaaggaacga agaagattgg atcataacca
    14281 aagccaccag tgcccgccgg ttcgcgagta atcacgcccg gccagctgcc gtggcatacc
    14341 agcggagtcg gatcttccgc gtgacgcaga tataccagca cgcagtggaa acgcgcctga
    14401 cgttggtcgt ccggtacgtc tttcagtgtt tccaacagtt tttgcagatt cttttgatcg
    14461 gtcgcgtctt caccggaata acgcgcggag taaatacccg gcgcgccacc cagcgcatct
    14521 accgccagac cagagtcgtc ggcaattgcc ggtaaaccgg tcacttttgc cgcatggcgc
    14581 gctttcagaa tcgcgttttc gataaaggtc aggccggttt cttcagcgga atcaacgccg
    14641 aggtctgttt gggccacgat atcaagaccg aagtcgctaa gcagcgacgc cagctcacgc
    14701 actttaccgg cattgccggt tgcgaggaca actttttgca taggatacct aattaattaa
    14761 cgccgcgatt tctggcggga tttgttgcgg attaatgatt ttaatttgtt tgtggcggcc
    14821 aagttcgcct ttttcaatta ccacctggct tttggctacc cggaattgct taccgagaaa
    14881 cttcaccaga tggctgttgg cctggccgtc aaccggcggc gcggtaatgg cgactttgac
    14941 ttcgtcgcca tgtaaaccga caatagaatc acggctggct ttcggctgaa tatagagccg
    15001 taaaaccaga ccgtcatcat taactgttac ggcactcata acgccatcca cagccccggc
    15061 agcagcatgt ttccggttgc ctgtaatact tctgcgacac ccatattgat gacatacagc
    15121 agcagaacga ggatcatcgg cgagaaatca atcccaccca ttgccggtag caggcggcga
    15181 atcgggcgca gcagcggatc ggccagctga atcagcacgt attcaatcgg gctacgaccc
    15241 tggcttaccc agctcataat cgccatcacc agcaggaccc agaaaatcag caggccgatg
    15301 gttttcagca gaatcagtaa accagcaatc cagatgattg gcaggaaggt caccacttta
    15361 aacagcacga tggctttgat aaaactgaga atgtacgcaa ccagcagcga ggcgctatca
    15421 atgggaccca ttgccggaat aacgcggcgc agtggcccga taattggctg tgttaccttc
    15481 actacaaact gtgagaaggg gttgtaaaaa tcacaatgag cccactgcat ccagatgcgt
    15541 aataacagca ccatggtata cagctcaatg accgttgaaa gcaggaaagt caacgtattc
    15601 atggcgttcc tttaattcct tattttttag agtaatcacg cgcaccaaaa attgcagtgc
    15661 cgatacgaac catcgtgcta cctgccgcaa tggcggcttc catatcgtcc gacattccca
    15721 gagagagcgt gtcgatatgc gggtagcgcg ttttcagtcc ggcaaatgct acagccattt
    15781 ggcgtgcaac ttcaaactgc cttacatatt ctgactcagg cgcagggatt gccatcagcc
    15841 cgcgcagacg taaacgcggt agttcagcga ccgccgctgc cagctcgtcc agttctgcca
    15901 gttgaatccc ggacttactg ttttcatcac taatattaat ttgaatcagt acgttaagag
    15961 ggggaagttc tgccgggcgc tgatcgttga ggcgggtagc aatgcgcaaa cggtcgatgg
    16021 tatgacacca gtcgaaatgc tctgccacca ggcggctttt attagactgc aacgggccaa
    16081 taaaatgcca ttctaatcct gttacgccca gttcctgaaa gtggcgaatt ttatctaccc
    16141 cttcctgaac gtagttttca ccaaattgac gctgcccggc atcaatggct tctgcgatgg
    16201 cgctcgcagg ttttgtttta ctgactgcaa gcagcgtaat ttcttctgga gaacggccgc
    16261 aacgcgttgc agccgctgag attttgtccc ggacctgtgc caggttatgc gcaatatcgt
    16321 tcattttccg aggatcttag tatgaatatg gaagaaattg tggcccttag tgtaaagcat
    16381 aacgtctcgg atctacacct gtgcagcgcc tggcccgcac gatggcgcat tcgcgggcta
    16441 atggaagctg cgccgtttga tgcgccggac gtcgaagagc tactgcggga gtggctggat
    16501 gacgatcagc gggcaatatt gctggaaaat ggccagctgg attttgccgt gtcgctggcg
    16561 gaaaaccagc ggttgcgtgg cagtgcgttc gcgcaacggc aaggtatttc tctggcatta
    16621 cggttgttac cttcgcactg tccacagctc gaacagcttg gcgcaccaac ggtattgccg
    16681 gaattactca agagcgagaa tggcctgatt ctggtgacgg gggcgacggg gagcggcaaa
    16741 tctaccacgc tggcggcgat ggttggctat ctcaatcaac atgccgatgc gcatattctg
    16801 acgctggaag atcctgttga atatatctat gccagccagc gatgtttgat ccagcagcgg
    16861 gaaattggtt tgcactgtat gacgttcgca tcgggattgc gggctgcatt gcgggaagat
    16921 cctgatgtga ttttgctcgg agagctgcgt gacagcgaga caatccgtct ggcactgacg
    16981 gcggcagaaa ccgggcattt ggtgctggca acattacata cgcgtggtgc cgcgcaggca
    17041 gttgagcgac tggtggattc atttccggcg caggaaaaag atcccgtacg taatcaactg
    17101 gcaggtagtt tacgggccgt gttgtcacaa aagctggaag tggataaaca ggaaggacgc
    17161 gtggcgctgt ttgaattatt gattaacact cccgcggtgg ggaatttgat tcgcgaaggg
    17221 aaaacccacc agttaccgca tgttattcaa accgggcagc aggtggggat gttaacgttt
    17281 cagcagagtt atcagcagcg ggtgggggaa gggcgtttgt gacgttgcca tgcccgatgc
    17341 gcaagtttat agggcctata aaggcaataa atgtctgatg cgacgctaac gcgtcttatc
    17401 agacctacag ccgctgccag atgtgtactg gataaggcgt tcacgccgca tccggcaaga
    17461 tggcggtaac gactatccgc caggctttta gatcgcctta atatccctgc tcgaaatagc
    17521 tttcgagaat aataaccgca gaggcagagt cgactttgcc tttgttgagc gcccgatagc
    17581 cgccctgttc aaacagaccg gaacgggctt ccacggtgct aagacgttcg tcatggagct
    17641 ttacttcaac accgaaacga ccatgaatac ggttggcaaa tttacgcgct ctggcagtca
    17701 atggttgctc ggtgccgtcc atattcagcg gcaaaccgac gatgatttcg tccggctgcc
    17761 actctttcag taaacgttcg atgaggttcc agtccggcgt accgtcctgt gctttaattg
    17821 caggcaaagg gcgagcagtg ccggtaatgc gttggccgac cgctacgcca atgcttttgg
    17881 tgccgaagtc gaaggcgagt aaggttccac tcatcaggcg tgccctgcca caccaggcat
    17941 ggtgagaata tccacaccaa tcagttttgc cgcctcgcgc cagcggtcgg caatcggcgt
    18001 tttgaacaga atattcagat ccgccggggc cgttagccac gcgttatcga gaatttcttg
    18061 ttccagttga cctttttccc aggaggcata acccagagcc accaatacgt cagacggttg
    18121 tttatcggtg ccgagcgttt ccagcacatc gcgggaagtg gtcatcactg tgttgtctga
    18181 aatgcgaatg ctggaagcaa aattggaggg cggagtatgc aaaataaacc cgcgatcttc
    18241 agccagcgga ccgccgagca taaccggttt atccagacgg attgattcat cacgcggctc
    18301 cggcgtgatc ttcagctttt ccagaatccc ttcaattttg agattttcca gcggcttgtt
    18361 gacgatgatc cccattgcac cattggtatt atgttcgcaa atgtagacca cggaacgacg
    18421 gaaaatcgga tcctggagag caggcatggc aataagaaag tgatgctgta aattcattgt
    18481 cagaggttct gtttcctggt tcaaaaaagc aacagcgccc agtatgcgtg gaaagaaggg
    18541 cgctgtcact cagagtctca acgagatcct tctcgctaag gtgggttact gctgctgtaa
    18601 acgtgcttcg atggcatcca ttaacattcc ggtgatcgac accggaaact ctgcttcaat
    18661 ctcacgaata caggttgggc tggtgacgtt aatttcagtc agacggtcgc cgatgatatc
    18721 cagaccaaca aaaatcagcc ctttttcttt cagcgtcggc ccgatctgac gggcgatttt
    18781 ccagtcactt tccgtcagcg gacgaggttc accgcgacca ccggcagcca gattgccacg
    18841 ggtttcgccc ccctgcggaa tacgcgccag gcagtacggt accggctcgc catccaccac
    18901 cagcacgcgt ttgtcgccat ctttaatggc tggcaggtaa ttttgcgcca tgcagtagcg
    18961 agtgccatgc tcagtcaggg tttcggcaat cacgccgagg tttggatcgc cttctttcac
    19021 gcggaaaatc gacgcgccgc ccataccgtc cagcggctta agaatgatgt cgctgtgttt
    19081 ctcccagaac gcttttagct gcgctttatt gcgcgtaacc agcgtttctg gcgttaagtc
    19141 agagaaccag gcggtaaaca gtttctcgtt acagtcgcgc aggctctgcg gcttgttaac
    19201 gatcagcgtc cctttctctt cggcacgttc cagaatatag gtcgcgtaga taaactcggt
    19261 atcaaacggc ggatctttac gcatcaggat cacatcgaga tcggccagcg gcagatcctg
    19321 ttcaccgacg aatgaaaacc actcttcgta gttctgcttc acgttcagcg tgcgggtatg
    19381 ggcgcgggct tcgccattga tcagatacag atcgcccatc tccatatagt gaagttcgta
    19441 accacgacgc tgtgcttcca gcaacatagc gaaactggaa tctttcttga tgttgatgtt
    19501 tgcgatgggg tccatcacga tgccgagctt gatcattatt cttctccgtt agcccaaatc
    19561 gccaaatcgt acttgtagcg cggtaatggc ggtgagcgca gttgtctctg tacgcaaaac
    19621 gcgaggtccc aacaggatat cagtaaattg atagcgggca gtcatggcaa tttcatctgc
    19681 cgataaaccg ccttccgggc caatcagcag gcggacgcgt tcaaccggta acggcaacgt
    19741 attgatgctg ttactggcgc gcgggtgaag attcagtttc agtccttcat cctgctctgc
    19801 acaccaggct tccagatcca tcgctgggcg gatttccggc acccggttac gaccacactg
    19861 ctcacaggca gcaattgcaa tcttctgcca ctgctggagc ttcttgttca gacgttcact
    19921 atccagttta acgccgcagc gctcagaaaa aagtggcgta atgaggctta caccgagttc
    19981 gatcgatttc tggatcgtaa attccatttt ttcaccacgc gacatcacct gaccgaggtg
    20041 aatatgcagc ggagattcgc gatcgtcgat cttgccttcc agcaccttca cttccacgct
    20101 ttttttgctg gcgctggtaa tttcggcgtc aaagacctgg ttgctaccgt caaacaattg
    20161 caacgcctgc cccggcccca tgcgcagtac gcgcccgata tggttggcgg catcttcgca
    20221 aagcgcgatg tgagaatggc tggtcagtgg ttcaggatga taaatgcggg ggatacgcat
    20281 agttaaaaat ccgcgtcgtc tccccacgcg gttgtacgcg tggggtaggg gttaacaaaa
    20341 agagtcccgc tagtgtaggt tagctctttc gcgcctggca agcgcgttgc acatacggat
    20401 tatgattgcc ttgcaccttc gcgatgcgtt catcgcgctc gcactcccag tcggtaaccg
    20461 gatacatctt gttccatgcg ttgaacagct gcgtttgctg gcgagagagt gtcaggttgt
    20521 attggtcgcg catatagaag taggtgcgtg caatggcacc gcgtgcacgc gctggtggtt
    20581 cggcagcttt ttctttgaaa tcgaccttca tggcgcattg accgtactgg ccttcgccgc
    20641 cattccactg gctgtacata aagttgccgc gatcgccatt cacctcaccg actgacggct
    20701 gcaggttatg catatcgctt tccatcttgc gatagaccgg atctttagcg cagtttttac
    20761 gtccaccgtc ctgccagcac tggcgctggt gaccgaactg ccaggcggga acgacgtgtt
    20821 cccactctac gcggctggct cggttttcat ttttgcgcac ctgatagccg cacgattgca
    20881 gatcaacaac gccttttttg ccctgccagt taattttaca tccgcaataa aacgtaccgg
    20941 gcgcgtcagc gtggactttt accgccgcgg ctttcgcctg agaaaaacta ttgatacctg
    21001 cggccaacgc cgggccggaa aatgctgcgc tcagtaccac cgcagcaata gacaaataac
    21061 ggtacatcac ttactccgtg ttaaaacgag ccagcaacgt agcgaaagtt gttttggtat
    21121 gcaatcagcc agatcaggaa agttcctgat agttcagatg gttatttcgc aaccagctgt
    21181 tcaccgcagt gaacacagcg atagacggcc tcgccacgaa ctacgcgatt atggcggcgt
    21241 acggtaagct gatgctcctg gcacttgcag cggtagggga aggtgttgcg acgcacggat
    21301 tgcagttcga actgatgcgt acgacgggcg ggaacgccca gcacgctttc catcatccac
    21361 ttccactctt tgccatgtgg cgctacgcgg ccgaaatgtt tccataccag caaatgtgcc
    21421 agttcgtgcg gtaccacttc ttcaataaaa gcttcactgt tttccaacag caaaacggga
    21481 ttgaggcgga tttcatagct ttccagccag gctgttccgg cggaggttcc gcgctgggtg
    21541 taagagagtt ttggctccgg gtagttacgc cctagcttca ggttggcctg ggcgagtttt
    21601 tcccgcaggc ggcgcataac ggcctgctgg atggcgatag ggagacggga tgttttcata
    21661 gcggcagagg atagagcgaa gaaaagatga ctgcaagagg tggcttcctc cgcgatggga
    21721 ggaagcctga ggagattaat cgtgagcgcc gatttcgcgc agtttacgac ctttcatcag
    21781 attacgttca atatgttcca gcgaaacgtg tttggtttcc ggtaccagcc acaatgtcag
    21841 caggataaac agtacgttca gagccgcata cacccagaag gtgttggcgt tacccagcgt
    21901 gttgagcatg gtcaggaacg ttgcgccaac gatcatgttg gcaatccagt tggtggcggt
    21961 ggagcaggtg atgccaaaat cgcggccttt cagcggctga atttcggagc acagtaccca
    22021 aatcagcgga ccggcactca tggcaaaacc gacaataaac atcagcagca tggcgatggc
    22081 gaaatactgc gctgacggag agtgaatacc gatatgcatc attgtaccga gtacgcccat
    22141 gccagcagcc atcaccagga agcccagcgt tagcgttggt ttacgtcccc agcggtcaac
    22201 aaggccgatt gcgataaagg tggcaagtac gttggtcagg ccgacaatca cggtccccca
    22261 catttgctcg gtagtgttgg tataacccgc cagttcgaag attttcggcg cgtaatacat
    22321 gatgacgttc atcccggtga attgctgcat tacctgcaac agtacgccaa ggaacaccgc
    22381 gcggcggaag ttctgttctc tttaaacagc gcccagccac tctgtttaac ctgcaaactt
    22441 tcacggattt catccagttc gcgtttcgct tccgcgctgg tgtcacgcag gcgtagcagc
    22501 acgcgttcgg catcaacaaa acggcgtttg gcggcaaacc aacgtgggct gtctggcagg
    22561 aagaagacac caatcagcag caaaattgcc gggatgataa tcacacccag catccagcgc
    22621 catgcaccgg tgtagctgaa ggcggtatca gaaagataag caccgaggat cccgatagtg
    22681 atcatcaact gatacatcga gatcatactg ccacgaattt tttccggcgc aatttcagag
    22741 aggtacagcg gtgcggtata agaggccaca cccaccgcca gccccagtag aacgcgggaa
    22801 agaatcagta cttcaacgtt tggcgcagcc gcagagaaca gcgaaccggc aacaaacaaa
    22861 attgcgccga tcatcaggct ctttttgcgc ccgagtttaa aggagagcca gccgctgccc
    22921 accgcaccga ctgccgcacc gaacatcatg gagcttacga cccattcttg cgtatgcgaa
    22981 gtaatctgga attcatctgc aataaacggc agtgcgccag caattacacc gatatccagg
    23041 ccaaagagta atcccgccag agcggcaagg aagcagacga aaaacgtcat tgccttgttt
    23101 gaccgcccct gttttttagc gtcaggcatg atgccctcca atatggttat tttttattgt
    23161 gaattaagat aggtgagtac gacgtaaaaa gatgtgaagc aaatcacatc agtgtaatcg
    23221 cttacactaa catttatctt agttatggtt taagtacatg aaaataattg agttatatca
    23281 tcatagatta aacgctgtta tctgcaatta agactttact gaaaagaaat gtaacaactg
    23341 tgaaaactga ataataatat gaaaatatta caaatatttg tctggtgttg ttggtgtaat
    23401 cggtttcagt agtgatgata atttgcgata aaacgtgttc aggaagaatg atgttattgt
    23461 gcgggcagct ttttgccgga tgcggcgtga acgcctatcc accgcggtga ggtaaaagaa
    23521 aaaggccagc gcgaggctgg cctttgaaag caggtgaaga aagattactt cagaccggca
    23581 gcttcacgca gcagctgcgc tttgtcggtt ttttcccacg ggaaatgttc acgaccaaag
    23641 tgaccgtatg ctgcggtttc tttgtagatc gggtgcagca gatccagcat ctgaatcaga
    23701 ccgtatgggc gcaggtcgaa gaactcacgt accagcaggg tcagctgttc agaaggcact
    23761 ttctcagtac cgaaggtttc taccatgatg gaggtcggtt cagccacgcc gattgcgtag
    23821 gaaacctgaa tttcacaacg atcggccagg ccagcagcaa cgatgttttt cgcgacataa
    23881 cgtgctgcgt acgctgcgga acggtccact tttgatggat ctttaccaga gaatgcaccg
    23941 ccaccgtgac gcgccatgcc gccgtaggta tcaacgataa ttttacgacc agtcagaccg
    24001 cagtcaccca ttgggccacc gataacgaaa cgaccggtcg ggttgatgaa gaatttggtg
    24061 gcagaagtca gccattcagc gggcagaatt ggcttgatga tctcttccat tactgcttct
    24121 tgcagcgatt tctggtcgat ctcttcagag tgctgagtgg aaagcacgac agcatcaata
    24181 ccaacgattt tgccgtcgtc atactggaaa gtcacctggc ttttcgcgtc cgggcgcagc
    24241 cacggcagag taccgttttt acgcacttca gcctgacgct gtaccagacg gtgtgcatag
    24301 gtgataggtg ctggcatcag cacgtcggtt tcattagttg cgtagccaaa catcagaccc
    24361 tggtcacccg cgccctgttc cagcggatcg gcacggtcaa cgccctggtt gatgtcagga
    24421 gactgtttgc cgatagcgct cagaaccgca caggagttag cgtcaaagcc catgtcggaa
    24481 tgtacatagc caatttcgcg aacggtgtta cgggtgatct cttcgatgtc tacccaggcg
    24541 ctagtggtga tttcgccgcc aactaaaacc atgccggttt ttacgtaggt ttcgcaagca
    24601 acgcgtgctt tcggatcctg ttcgaggatc gcgtctaaaa cggcatcaga aatttggtca
    24661 gcaattttgt caggatgccc ttcagagacg gactcggacg taaaaaggtg ttttgccata
    24721 tttaatatca cctaaagaga atttggttag ctcaaactgt tgtgtggatt ttctgtggta
    24781 gcggatccta ccacgactct gcaggttaaa aacactggca gtctgagtgt taatcggtat
    24841 ggatggatta acatctggat ggctatttta ggtcaattct tcacccgatt tccacttttt
    24901 tttgaatcgt gtctcattct gttaaaaacg tggctggaaa tttttcctga caatgccggc
    24961 attctgcgta tttatctttt gcaattttct gccattgtgg ggtataaaac gcggcgcgcg
    25021 gcttaaataa aaagcacacg acgtttcttt cgtgttgcca cttccagccg ggttgaaatc
    25081 agagttttgg cttgtgggtt cgtcttaaca ggcggccgtg gaggtgatac gaaataatga
    25141 accgttgtct gctgcttaac ctgtctcacc gttctggtga agattcgttc cccgcactct
    25201 gcatctctgc tttgcatacc tgccgatgtt atacccatct cggcgcttct caggattcaa
    25261 gagctggtta cagttactga ggactgaaca agggcgctct tgtaaaaaca agagttttct
    25321 cgtggtttcg ccgaactgtc acacttacgt tcggttatgt gcttaataat gttatgaaaa
    25381 agaaaccggt tgcgcagtcg gagcgtcagc atacactgct ggaaaatcca tgtgcttatg
    25441 ggttgttatc gcagttccag gctgcgatag tcgttaactg ttttacactt aataaaataa
    25501 tttgaggttc gctatgtctg acgacatgtc tatgggtttg ccttcgtcag cgggcgaaca
    25561 cggtgtacta cgctccatgc aggaggttgc aatgagctcc caggaagcca gcaagatgct
    25621 gcgtacttac aatattgcct ggtggggcaa taactactat gacgttaacg agctgggcca
    25681 cattagcgtg tgcccggacc cggacgtccc ggaagctcgc gtcgatctcg cgcagttagt
    25741 gaaaactcgt gaagcacagg gtcagcgtct gcctgcactg ttctgtttcc cacagatcct
    25801 gcagcaccgt ttgcgttcca ttaacaccgc gttcaaacgt gcgagggaat cctacggcta
    25861 taacggcgat tacttccttg tttatccgat caaagttaac cagcaccgcc gcgtgatcga
    25921 gtccctgatt cattcgggcg aaccgctggg tctggaagcc ggttccaaag ccgagttgat
    25981 ggcagtgctg gcacatgctg gcatgacccg tagcgtcatc gtctgcaacg gttataaaga
    26041 ccgcgaatat atccgcctgg cattaattgg cgagaagatg gggcacaagg tctatctggt
    26101 cattgagaag atgtcagaaa tcgccattgt gctggatgaa gcagaacgtc tgaatgtcgt
    26161 tcctcgtctg ggcgtgcgtg cacgtctggc ttcgcagggc tcgggtaaat ggcagtcctc
    26221 cggcggggaa aaatcgaagt tcggcctggc tgcgactcag gtactgcaac tggttgaaac
    26281 cctgcgtgaa gccgggcgtc tcgacagcct gcaactactg cacttccacc tcggttcgca
    26341 gatggcgaat attcgcgata tcgcgacagg cgttcgtgaa tccgcgcgtt tctatgtgga
    26401 actgcacaag ctgggcgtca atattcagtg cttcgacgtc ggcggcggtc tgggcgtgga
    26461 ttatgaaggt actcgttcgc agtccgactg ttcggtgaac tacggcctca atgaatatgc
    26521 caacaacatc atctgggcga ttggtgatgc gtgtgaagaa aacggtctgc cgcatccgac
    26581 ggtaatcacc gaatcgggtc gtgcagtgac tgcgcatcac accgtgctgg tatcgaatat
    26641 catcggcgtg gaacgtaacg aatatacggt gccgaccgcg cccgcagaag atgcgccgcg
    26701 cgcgctgcaa agcatgtggg aaacctggca ggagatgcac gaaccgggaa ctcgccgttc
    26761 tttgcgtgaa tggttacacg acagccagat ggatctgcac gacattcata tcggctactc
    26821 ttccggcacc tttagcctgc aagaacgtgc atgggcagaa caactctatc tgagcatgtg
    26881 ccatgaagtg cagaagcaac tggatccgca aaaccgtgct catcgtccga ttatcgacga
    26941 gctgcaggaa cgtatggcag acaaaatgta cgtcaacttc tcgctgttcc agtcgatgcc
    27001 ggatgcgtgg gggatcgacc agttgttccc ggttctgccg ctggaagggc tggatcaagt
    27061 gccggaacgc cgcgctgtgc tgctggatat tacctgtgac tctgacggtg ctatcgacca
    27121 ctatattgat ggtgatggta ttgccacgac aatgccaatg ccggagtacg atccagagaa
    27181 tccgccgatg ctcggtttct ttatggtcgg cgcatatcag gagatccttg gcaatatgca
    27241 caacctgttc ggtgataccg aagcggttga cgtgttcgtg ttccctgacg gtagcgtaga
    27301 agtagaactg tctgacgaag gcgataccgt ggcggacatg ctgcaatatg tacagctcga
    27361 tccgaaaacg ctgttaaccc agttccgcga tcaagtgaag aaaaccgatc ttgatgctga
    27421 actgcaacaa cagttccttg aagagttcga ggcaggtttg tacggttata cttatcttga
    27481 agatgaataa ttaagattat tccgctggcg ggtcgattta tttacgtaaa tcggcccgct
    27541 ttctttttat atattcttaa aaataatctg gctgcttttc ttattaaata atctccctac
    27601 aatcgctgca ttaattaaat gcagagggaa cggttatgaa aaaatggaaa gtacgtagcg
    27661 cgttggtggc attaatcgtg ctgctggccg gttgttccag taatgcgcag tataatagca
    27721 gtgcatcggg caatgtcggc accgcctggg gtggggatgt gcactcatcg gtccagggcg
    27781 tatctgctga acgagcctgg cgcgatcctg cagagatgat cgttattagt tattcgacta
    27841 atgttccttc cggctatgac cgcgtttata gcattcgtat taacgaactg gaatatgcta
    27901 ttcgcgatgg taattttaat tctctaccta ttacgcgtgt ttatgattca tccaataatg
    27961 aaccacgcta tattgtccat gccagagtcg gaatgaatta tcaattatat gtccgtaatt
    28021 acagtcgtaa caccaactac gagatcgttg ctacggttga tggcatggat gtgctgaacg
    28081 gcaagcaagg ttcgctgaat aacaacggct atattgtgaa tgccggagat tcgttagcga
    28141 ttaaaggatt ccgcaaagat aaacataccg aagcggcttt tcagttcgcc aacgttgctg
    28201 attcctacgc agccaattcg gcgcaaggcg atgtgcgtaa taccggggta atcggctttg
    28261 ccgcctttga attacaaggc ccggcgcaaa acgcgctgcc gccgtgctct ggacaagcct
    28321 ttccggcaga taacaatggc tatgcgccgc caccgtgtcg gaaatagcaa cctcgcttga
    28381 tgcctcgccc atttaaggcg ataattagca caaacttgtg tttcgcaaat ccaacccttc
    28441 ctcgtcgggc ctaacgacgc ggaagggttt ttttatatcg actttgtaat aggagtccat
    28501 ccatgagcac cttaggtcat caatacgata actcactggt ttccaacgcc tttggttttt
    28561 tacgcctgcc gatgaacttc cagccgtatg acagcgatgc tgactgggtg attactggtg
    28621 tgccgttcga catggccacc tctggccgtg ccggtggtcg ccacggtccg gcagcgatcc
    28681 gtcaggtttc gacgaatctg gcctgggaac acaatcgctt cccgtggaat ttcgacatgc
    28741 gtgagcgtct gaacgtcgtg gactgcggtg atctggtata tgctttcggc gatgcccgtg
    28801 agatgagcga gaagctgcag gcgcacgccg agaagctgct ggctgccggt aagcgtatgc
    28861 tctccttcgg tggtgaccac tttgttacgc tgccgctgct gcgtgctcat gcgaagcatt
    28921 tcggtaaaat ggcgctggta cactttgacg cccacaccga tacctatgcg aacggttgtg
    28981 aatttgacca cggcaccatg ttctataccg cgccgaaaga aggtctgatc gacccgaatc
    29041 attccgtgca gattggtatt cgtaccgagt ttgatataga caacggcttt accgtgctgg
    29101 acgcctgcca ggtgaacgat cgcagcgtgg atgacgttat cgcccaggtg aaacagattg
    29161 tgggtgatat gccggtttac ctgacctttg atatcgactg cctggatcct gcttttgcac
    29221 caggcaccgg tacgccagtg attggcggcc tgacctccga tcgcgctatt aaactggtac
    29281 gcggcctgaa agatctcaac atcgttggga tggacgtagt ggaagtggct ccggcatatg
    29341 atcagtcgga aatcaccgct ctggctgcgg cgacgctggc gctggagatg ctgtatattc
    29401 aggcggcgaa aaagggcgag taagcaccag atgcgatgcg cacgggtaaa acgtgccatt
    29461 aatgtcggat gcggcgtgaa cgccttatcc gacctacgtt cggcacccgt aggccggata
    29521 agatgcgcca gcatcgcatc cggcaatgcg cacaaggtaa caaatgtgcc attcatgtca
    29581 gatgcggcgt gaacgcctta tctgacctac gttcgacacc accaggcttt acttaatccc
    29641 atccgcgctc atgcgatcgc gaatatgctg ggcgcgttcg gcggatgcag gatggtcgtc
    29701 aaacattgag ctttggcggc cttcttccag tttcgccagt ttttcaaagc tggtggcaag
    29761 acctgccggg ctgatgccgc gttggcgcag aagatcgtaa gaataatcat cggcttctgc
    29821 ttcctggcgc tgggagaatt gcgaattgac taatttctcg cccagatcac caagttgtga
    29881 ttgagataaa ctgccgacaa tcccgcccgc agaggccgca gctactcgca cggcatttgt
    29941 accaagcgcc acctgcatcc ctttcttcac atggcctaac gccacgtgcc ccatctcgtg
    30001 accgatcacc gcttcgactt cgttatccgt catcatatcc atcagcccgc tatagacgcg
    30061 gatacagccg ttagccattg caaaggcgtt cacatccttc gccatataca ctttgtaatt
    30121 caccggctga ccgttgatat tgttgcctag cgcattggca atagttgtca gacgtttagc
    30181 gtattcgcta ttggctggcg caatcgtcgc cttgctgtcc atctcctgac atgcctgatc
    30241 gctcagggct ttcacctgcg catcactcaa actgtaagcc tgaaaagctt ccgctcctga
    30301 tgaaagcagt ccgttggagt ccatattctg gcaaccggtc agtaccgttg ccacgctcat
    30361 tgctaccaat aaggcgcgaa ttttcatgtt ttctcttccg cacaaggtca ggattattct
    30421 aaaaatgcgc cgtttgcagg tgaatcgacg ctcagtctca gtataaggaa aagcgcagcg
    30481 gacgggcgag tagattgcgc aacatgcgag catgatccag agatttctga agcggcaaaa
    30541 ggatgttcca tgtacatgac gcgcggcttg cggtaaattg ttggcaaatt ttccggcgta
    30601 gcccaaaacg cgctgtcgtc aagtcgttaa gggcgtgccc ttcatcatcc gatctggagt
    30661 caaaatgtcc tcacgtaaag agcttgccaa tgctattcgt gcgctgagca tggacgcagt
    30721 acagaaagcc aaatccggtc acccgggtgc ccctatgggt atggctgaca ttgccgaagt
    30781 cctgtggcgt gatttcctga aacacaaccc gcagaatccg tcctgggctg accgtgaccg
    30841 cttcgtgctg tccaacggcc acggctccat gctgatctac agcctgctgc acctcaccgg
    30901 ttacgatctg ccgatggaag aactgaaaaa cttccgtcag ctgcactcta aaactccggg
    30961 tcacccggaa gtgggttaca ccgctggtgt ggaaaccacc accggtccgc tggggcaggg
    31021 tattgccaac gcagtaggta tggcgattgc agaaaaaacg ctggcggcgc agtttaaccg
    31081 tccgggccac gacattgtcg accactacac ctacgccttc atgggcgacg gctgcatgat
    31141 ggaaggcatc tcccacgaag tttgctctct ggcgggtacg ctgaagctgg gtaaactgat
    31201 tgcgttctac gatgacaacg gtatctctat tgatggtcac gttgaaggct ggttcaccga
    31261 cgacaccgca atgcgtttcg aagcttacgg ctggcacgtt attcgcgaca tcgacggtca
    31321 tgacgcggca tccatcaaac gcgcagtaga agaagcgcgc gcagtgactg acaaaccgtc
    31381 cctgctgatg tgcaaaacca tcatcggttt cggttccccg aacaaagccg gtactcacga
    31441 ctcccacggt gcgccgctgg gcgacgctga aattgccctg acccgcgaac agctgggctg
    31501 gaaatatgca ccgttcgaaa tcccgtctga aatctatgct cagtgggatg cgaaagaagc
    31561 aggccaggcg aaagaatccg catggaacga gaaattcgct gcttacgcga aagcttatcc
    31621 gcaggaagcc gctgaattta cccgccgtat gaaaggcgaa atgccgtctg acttcgacgc
    31681 gaaagcaaaa gagttcattg ctaaactgca ggctaacccg gcgaaaatcg ccagccgtaa
    31741 agcgtctcag aatgctatcg aagctttcgg tccgctgttg ccggaattcc tcggcggttc
    31801 tgctgacctg gcaccgtcta acctgaccct gtggtctggt tctaaagcaa tcaacgaaga
    31861 tgctgcgggt aactacatcc actacggtgt tcgcgagttc ggtatgaccg cgattgctaa
    31921 cggtatctcc ctgcacggtg gtttcctgcc gtacacctcc accttcctga tgttcgtgga
    31981 atacgcacgt aacgccgtac gtatggctgc gctgatgaaa cagcgtcagg tgatggttta
    32041 cacccacgac tccatcggtc tgggcgaaga cggcccgact caccagccgg ttgagcaggt
    32101 cgcttctctg cgcgtaaccc cgaacatgtc tacatggcgt ccgtgtgacc aggttgaatc
    32161 cgcggtcgcg tggaaatacg gtgttgagcg tcaggacggc ccgaccgcac tgatcctctc
    32221 ccgtcagaac ctggcgcagc aggaacgaac tgaagagcaa ctggcaaaca tcgcgcgcgg
    32281 tggttatgtg ctgaaagact gcgccggtca gccggaactg attttcatcg ctaccggttc
    32341 agaagttgaa ctggctgttg ctgcctacga aaaactgact gccgaaggcg tgaaagcgcg
    32401 cgtggtgtcc atgccgtcta ccgacgcatt tgacaagcag gatgctgctt accgtgaatc
    32461 cgtactgccg aaagcggtta ctgcacgcgt tgctgtagaa gcgggtattg ctgactactg
    32521 gtacaagtat gttggcctga acggtgctat cgtcggtatg accaacttcg gtgaatctgc
    32581 tccggcagag ctgctgtttg aagagttcgg cttcactgtt gataacgttg ttgcgaaagc
    32641 aaaagaactg ctgtaattag catttcgggt aaaaaggtcg cttcggcgac cttttttatt
    32701 accttgatat gtccgtttgc ggacaagcaa tagataaagc gtgttgtaga tcacaaatat
    32761 ttatatgcaa taaat
//
LOCUS       DAZGSP010000052        32619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZGSP010000052 DAZGSP010000000
VERSION     DAZGSP010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.052"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            43..1332
                     /gene="hpaG"
                     /locus_tag="ACHK74_002689"
     CDS             43..1332
                     /gene="hpaG"
                     /locus_tag="ACHK74_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFP0221478.1"
                     /translation="MKGTIFAVALNHSSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            1329..2795
                     /gene="hpaE"
                     /locus_tag="ACHK74_002690"
     CDS             1329..2795
                     /gene="hpaE"
                     /locus_tag="ACHK74_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFP0221479.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2797..3648
                     /gene="hpaD"
                     /locus_tag="ACHK74_002691"
     CDS             2797..3648
                     /gene="hpaD"
                     /locus_tag="ACHK74_002691"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFP0221480.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGIYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3658..4038
                     /locus_tag="ACHK74_002692"
     CDS             3658..4038
                     /locus_tag="ACHK74_002692"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFP0221481.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            4106..4909
                     /gene="hpaH"
                     /locus_tag="ACHK74_002693"
     CDS             4106..4909
                     /gene="hpaH"
                     /locus_tag="ACHK74_002693"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFP0221482.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4920..5708
                     /gene="hpaI"
                     /locus_tag="ACHK74_002694"
     CDS             4920..5708
                     /gene="hpaI"
                     /locus_tag="ACHK74_002694"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFP0221483.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATTVKPGVY"
     gene            5883..7259
                     /gene="hpaX"
                     /locus_tag="ACHK74_002695"
     CDS             5883..7259
                     /gene="hpaX"
                     /locus_tag="ACHK74_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFP0221484.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSLVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMIYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7269..8159
                     /gene="hpaA"
                     /locus_tag="ACHK74_002696"
     CDS             7269..8159
                     /gene="hpaA"
                     /locus_tag="ACHK74_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFP0221485.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFHMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8410..9972
                     /gene="hpaB"
                     /locus_tag="ACHK74_002697"
     CDS             8410..9972
                     /gene="hpaB"
                     /locus_tag="ACHK74_002697"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFP0221486.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            9990..10502
                     /locus_tag="ACHK74_002698"
     CDS             9990..10502
                     /locus_tag="ACHK74_002698"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFP0221487.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10895..13045
                     /gene="btsT"
                     /locus_tag="ACHK74_002699"
     CDS             10895..13045
                     /gene="btsT"
                     /locus_tag="ACHK74_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFP0221488.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLNVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13095..13298
                     /gene="yjiX"
                     /locus_tag="ACHK74_002700"
     CDS             13095..13298
                     /gene="yjiX"
                     /locus_tag="ACHK74_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP0221489.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13309..14265
                     /gene="yjiA"
                     /locus_tag="ACHK74_002701"
     CDS             13309..14265
                     /gene="yjiA"
                     /locus_tag="ACHK74_002701"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFP0221490.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(14343..16883)
                     /locus_tag="ACHK74_002702"
     CDS             complement(14343..16883)
                     /locus_tag="ACHK74_002702"
                     /EC_number="3.5.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008953 - penicillin amidase activity
                     [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin G acylase"
                     /protein_id="HFP0221491.1"
                     /translation="MKNRNRMIVNCVTASLMYYWSLPALAEQSSSEIKIVRDEYGMPH
                     IYANDTWHLFYGYGYVVAQDRLFQMEMARRSTQGTVAEVLGKDFVKFDKDIRRNYWPD
                     AIRAQIAALSPEDMSILQGYADGMNAWIDKVNTNPETLLPKQFNTFGFTPKRWEPFDV
                     AMIFVGTMANRFSDSTSEIDNLALLTALKDKYGVSQGMAVFNQLKWLVNPSAPTTIAV
                     QESNYPLKFNQQNSQTAALLPRYDLPAPMLDRPAKGADGALLALTAGKNRETIAAQFA
                     QGGANGLAGYPTTSNMWVIGKSKAQDAKAIMVNGPQFGWYAPAYTYGIGLHGAGYDVT
                     GNTPFAYPGLVFGHNGVISWGSTAGFGDDVDIFAERLSAEKPGYYLHNGKWVKMLSRE
                     ETITVKNGQAETFTVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAK
                     NWQEWTQQAAKQALTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKG
                     LLPFEMNPKVYNPQSGYIANWNNSPQKDYPASDLFAFLWGGADRVTEIDRLLEQKPRL
                     TADQAWDVIRQTSRQDLNLRLFLPTLQAATSGLTQSDPRRQLVETLTRWDGINLLNDD
                     GKTWQQPGSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTGSLNISVGA
                     KILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVSNWKTPAMA
                     LTFRANNFFGVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWDVVAPGQSG
                     FIAPDGTVDKHYEDQLKMYENFGRKSLWLTKQDVEAHKESQEVLHVQR"
     gene            complement(17235..17399)
                     /gene="yjiS"
                     /locus_tag="ACHK74_002703"
     CDS             complement(17235..17399)
                     /gene="yjiS"
                     /locus_tag="ACHK74_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFP0221492.1"
                     /translation="MEFHENKAKTPFIRLVQLWHAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            17576..18988
                     /gene="yjiR"
                     /locus_tag="ACHK74_002704"
     CDS             17576..18988
                     /gene="yjiR"
                     /locus_tag="ACHK74_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP0221493.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMRLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DNSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQSYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(19230..20174)
                     /locus_tag="ACHK74_002705"
     CDS             complement(19230..20174)
                     /locus_tag="ACHK74_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFP0221494.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVILLVTECANDSQITALLNYIL
                     LTGDEARFKKFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            20641..21873
                     /gene="mdtM"
                     /locus_tag="ACHK74_002706"
     CDS             20641..21873
                     /gene="mdtM"
                     /locus_tag="ACHK74_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFP0221495.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            21914..23194
                     /gene="yjiN"
                     /locus_tag="ACHK74_002707"
     CDS             21914..23194
                     /gene="yjiN"
                     /locus_tag="ACHK74_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFP0221496.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKLIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            23310..24461
                     /gene="yjiM"
                     /locus_tag="ACHK74_002708"
     CDS             23310..24461
                     /gene="yjiM"
                     /locus_tag="ACHK74_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFP0221497.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDTSRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            24471..25238
                     /gene="yjiL"
                     /locus_tag="ACHK74_002709"
     CDS             24471..25238
                     /gene="yjiL"
                     /locus_tag="ACHK74_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFP0221498.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            25235..25492
                     /gene="ytiA"
                     /locus_tag="ACHK74_002710"
     CDS             25235..25492
                     /gene="ytiA"
                     /locus_tag="ACHK74_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFP0221499.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            25557..26417
                     /gene="yjiK"
                     /locus_tag="ACHK74_002711"
     CDS             25557..26417
                     /gene="yjiK"
                     /locus_tag="ACHK74_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFP0221500.1"
                     /translation="MTKSNSLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            26485..27663
                     /gene="yjiJ"
                     /locus_tag="ACHK74_002712"
     CDS             26485..27663
                     /gene="yjiJ"
                     /locus_tag="ACHK74_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0221501.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMNWWLLAIL
                     YGLAGFGYIIVATFLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(27676..28230)
                     /gene="kptA"
                     /locus_tag="ACHK74_002713"
     CDS             complement(27676..28230)
                     /gene="kptA"
                     /locus_tag="ACHK74_002713"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFP0221502.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            28480..29163
                     /gene="yjiH"
                     /locus_tag="ACHK74_002714"
     CDS             28480..29163
                     /gene="yjiH"
                     /locus_tag="ACHK74_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFP0221503.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            29160..29621
                     /gene="yjiG"
                     /locus_tag="ACHK74_002715"
     CDS             29160..29621
                     /gene="yjiG"
                     /locus_tag="ACHK74_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFP0221504.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            29634..30806
                     /gene="iadA"
                     /locus_tag="ACHK74_002716"
     CDS             29634..30806
                     /gene="iadA"
                     /locus_tag="ACHK74_002716"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP0221505.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            30871..31782
                     /gene="hypT"
                     /locus_tag="ACHK74_002717"
                     /gene_synonym="qseD"
     CDS             30871..31782
                     /gene="hypT"
                     /locus_tag="ACHK74_002717"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFP0221506.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(31775..32167)
                     /gene="iraD"
                     /locus_tag="ACHK74_002718"
     CDS             complement(31775..32167)
                     /gene="iraD"
                     /locus_tag="ACHK74_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFP0221507.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNFKEAYCHTLAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(32164..32247)
                     /gene="idlP"
                     /locus_tag="ACHK74_002719"
     CDS             complement(32164..32247)
                     /gene="idlP"
                     /locus_tag="ACHK74_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFP0221508.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(32363..32506)
                     /gene="ytiD"
                     /locus_tag="ACHK74_002720"
     CDS             complement(32363..32506)
                     /gene="ytiD"
                     /locus_tag="ACHK74_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFP0221509.1"
                     /translation="MADYAEINNFPPELSSSGDKYFHLRNYSEYSEYTSGFFLSLMIF
                     IKS"
BASE COUNT         7530 a         8586 c         8521 g         7982 t
ORIGIN      
        1 taacatatta atgattaaaa tgatcgaatc cgaggagtgg taatgaaagg cactatcttc
       61 gccgtagcgt tgaaccatag cagccaactt gatgcatggc aggaagcgtt ccagcaatcc
      121 ccctacaaag ccccgcctaa aactgcggtc tggtttatta aaccgcgcaa tacggtgatt
      181 ggttgcggtg aaccgattcc ctttccacag ggtgaaaagg tactgagcgg tgcgactgtt
      241 gcgctgattg tgggaaaaac ggcgacgaaa gtacgtgaag aagatgcggc agagtacatc
      301 gccggatatg cgctggctaa cgacgtcagc ctgccggaag agagctttta ccgcccggca
      361 atcaaagcaa aatgtcgtga tggattctgc cccattggcg aaaccgtggc tctcagcaat
      421 gtcgataatc tgaccatcta taccgagatc aacgggcgtc ctgccgatca ctggaatacc
      481 gccgatttac aacgtaacgc cgcgcagttg ctgagcgccc tgagcgaatt tgccacgctg
      541 aatccaggcg acgccattct gctcggcacg ccacaggcgc gcgtggaaat acagccaggc
      601 gatcgcgttc gtgttctcgc agaaggtttc ccgccgctgg aaaatccggt agtggacgaa
      661 cgtgaagtga ccacgcgcaa gagcttccca acgcagccac acccgcacgg cacgctgttt
      721 gccctcggcc tgaactacgc cgaccacgcc agcgaactgg aatttaagcc accggaagaa
      781 ccgctggtgt tcctgaaagc gccaaatacc ctcactggcg ataaccagac ctccgtgcgt
      841 ccaaacaata ttgaatacat gcactatgaa gcggagctgg tggtagttat tggcaaacag
      901 gcgcgtaacg tcagcgaagc cgatgccatg gattatgtcg cgggctacac cgtgtgtaac
      961 gactacgcca ttcgcgacta tctggaaaac tactaccgcc ctaacctgcg ggtcaaaagc
     1021 cgcgacggac tgacgccgat gctttcaacc atcgtgccga aagaggcgat cccggacccg
     1081 cataatctga cccttcgcac cttcgtcaac ggcgagttac gccagcaagg caccaccgcc
     1141 gatctgatct tcagcgtgcc cttcctgatc gcctacttaa gcgaatttat gaccctgaat
     1201 ccgggcgaca tgatcgccac cggcacacca aaaggcttat ctgacgtggt gcctggcgat
     1261 gaagtggtgg tggaagtaga aggcgtgggc cgcctggtga accgaattgt gagtgaggaa
     1321 acagcgaaat gaaaaaagta aatcattgga tcaacggtaa aaatgttgca ggtaacgact
     1381 acttccagac taccaatccg gcaacgggtg aagtgctggc ggatgtggcc tccggcggtg
     1441 aagcggagat caatcaggcg gtagcggcag cgaaagaggc gttcccgaaa tgggccaatc
     1501 tgccgatgaa agagcgcgcg cgcctgatgc gccgtctggg cgatctgatc gaccagaacg
     1561 tgccggagat cgccgcgatg gaaaccgcgg acacgggcct gccgatccat cagaccaaaa
     1621 atgtgttgat cccacgcgct tctcacaact ttgaattttt cgcggaagtc tgccagcaga
     1681 tgaacggcaa gacttatccg gtcgacgaca agatgctcaa ctacacgctg gtgcagccgg
     1741 taggcgtttg tgcactggtg tcaccgtgga acgtgccgtt tatgaccgcc acctggaagg
     1801 tcgcgccgtg tctggcgctg ggcaataccg cggtactgaa aatgtcggaa ctctccccgc
     1861 tgaccgccga ccgcctgggt gagctggcgc tggaagctgg tattccggcg ggcgtgctga
     1921 acgtggtaca gggctacggc gcaaccgcag gcgatgcgct ggtccgtcat catgacgtgc
     1981 gtgccgtgtc gttcaccggc ggtacggcga ccgggcgcaa catcatgaaa aacgccgggc
     2041 tgaaaaaata ctccatggaa ctgggcggta aatcgccggt gctgattttt gaagatgctg
     2101 atattgaacg cgcgctggac gccgccctgt tcaccatctt ctcgatcaac ggcgaacgct
     2161 gcaccgccgg ttcgcgcatc tttattcagc aaagcatcta cccggaattc gttaaacgct
     2221 ttgccgaacg cgccaaccgt ctgcgcgtgg gcgatccgaa cgatccgaat acccaggttg
     2281 gcgcgcttat cagccagcag cactgggaaa aagtctccgg ctatatccgt ctcggcattg
     2341 aagaaggcgc aaccctgctg gcgggcggcc cggataaacc gtccgacctg cctgcgcacc
     2401 tgaaaggcgg caacttcctg cgcccaaccg tgctggcaga cgttgataac cgtatgcgcg
     2461 tcgcccagga agagattttc gggccggtcg cctgcctgct gccgtttaaa gacgaagctg
     2521 aaggcttacg cctggcaaac gacgtggagt acggcctcgc gtcgtacatc tggactcagg
     2581 atgtcagcaa agtgctgcgt ctggcgcgtg gcattgaagc tggcatggtg ttcgtcaaca
     2641 cccagaacgt gcgtgatttg cgccagccgt ttggcggcgt aaaagcctcc ggcaccgggc
     2701 gtgaaggcgg tgagtacagc ttcgaagtgt tcgcggaaat gaagaacgtc tgcatttcca
     2761 tgggcgacca tccaattccg aaatggggag tctgatatgg gtaagttagc gttagccgcc
     2821 aagatcacgc acgtaccgtc gatgtatctc tctgaactgc cagggaaaaa ccacggttgc
     2881 cgccagggcg cgatcgacgg gcataaagag atcagcaagc gttgccggga aatgggcgtc
     2941 gataccatta tcgttttcga tacccactgg ctggtcaaca gtgcttatca catcaactgt
     3001 gcagaccatt ttgaaggcat ctacaccagc aacgagctgc cgcattttat ccgcgatatg
     3061 acctacaact acgagggcaa cccggagttg gggcagctta ttgccgatga agccttaaag
     3121 ctcggcgtgc gggcaaaagc gcacaacatt cccagtctga aactggaata cggcacgctg
     3181 gtaccgatgc gctacatgaa tgaagacaag cacttcaaag tggtctccat ttcggctttc
     3241 tgcacggttc acgattttgc tgacagccgc aagctgggcg aagcgattct gaaagcgatc
     3301 gaacagtacg acggcaccgt ggcggtcctt gccagcggtt cgttatcgca ccgctttatt
     3361 gacgatcagc gtgcagaaga agggatgaac agctacaccc gcgagttcga ccgccagatg
     3421 gacgagcgcg tggtgaagtt gtggcgcgaa ggccagttca aagagttctg caatatgctg
     3481 ccggagtacg ccgactactg ctacggcgaa ggcaatatgc acgacacggt gatgctgctg
     3541 gggatgctcg gctgggataa atacgacggc aaggtggagt ttatcaccga gctattccca
     3601 agctctggca ccggtcaggt taacgctgtt ttcccgcttc ccgcgtaagg aaccaccatg
     3661 ccgcacttta tcgtggaatg tactgaaaat attcgtgaag aagcccgctt acctgagctg
     3721 tttgccagtg ttaacactgc actggcggca acggggattt ttccgcttgg gggaatacgt
     3781 agccgggcgc actggatcga tacctggcaa atggctgatg gcaagcacga ttacgccttt
     3841 gtgcatatga cgctaaaaat cggcagcggg cgcagcctgg aaagccgtca ggaagtgggc
     3901 gaaatgctgt ttgatctgat taaaacgcac ttcgcctcac tgatggagag ccgttacctg
     3961 gcgctttcat ttgagattgc agagctgcac ccgacgctga attttaaaca aaacaacgtg
     4021 cacgcgttgt ttaaataaat ttgcaccagc ttccaggccg gatgataagg cgcagccgca
     4081 tccggcaatg ccacaggata tcgctatgtt cgataaacac acccacaccc tgatcgccca
     4141 gcgtctggat caggcagaaa aacagcgcga acagatccgc gcgatctcgc tggattaccc
     4201 ggagatcacc atcgaagacg cttacgcggt gcagcgtgaa tgggttcgac tgaaaatcgc
     4261 cgaaggtcgc acgctgaaag gccacaaaat cggcctgacc tcgaaagcga tgcaggccag
     4321 ctcacaaatc agcgagccgg attacggtgc cctgctggat gacatgttct tccacgatgg
     4381 cagcgatatc ccgaccgatc gctttatcgt gccgcgcatt gaagtggagc tggctttcgt
     4441 gctggcaaaa ccgctgcgtg ggccaaactg cacgctgttc gacgtctaca acgccacgga
     4501 ctatgtgatc ccggcgctgg agctgatcga cgctcgctgc cacaacatcg atccggaaac
     4561 ccagcgcccg cgcaaagtgt tcgacaccat ttccgataac gccgccaacg ccggggtgat
     4621 cctcggcggt cgtccgatca aaccagacga actggatctg cgttggatct ccgccctgat
     4681 gtatcgcaat ggcgtgattg aagaaaccgg cgtcgccgct ggtgtgctga atcatccggc
     4741 aaacggcgtg gcctggctgg cgaacaaact cgcaccgtat gacgtacaac tggaagccgg
     4801 gcaaatcatt ctcggcggtt cgttcacccg cccggttccg gcgcgtaagg gcgacacctt
     4861 ccacgtcgat tacggcaaca tgggctccat tagctgccgc tttgtttaag gagagaacga
     4921 tggaaaacag ttttaaagcg gcgctgaaag caggccgtcc gcagattgga ttatggctgg
     4981 ggctgagcag cagctacagc gcggagttac tggccggagc aggattcgac tggttgttga
     5041 tcgacggtga gcacgcgccg aataacgtgc aaaccgtgct cacccagcta caggcgattg
     5101 cgccctaccc cagccagccg gtagtacgtc cgtcgtggaa cgatccggtg caaatcaaac
     5161 aactgctgga cgtcggcaca caaaccttgc tggtgccgat ggtacaaaac gctgacgaag
     5221 cccgtgaagc ggtacgcgcc acccgttatc cccccgccgg tattcgcggt gtgggcagtg
     5281 cgctggcccg agcctcgcgc tggaatcgta ttcctgatta cctgcaaaaa gccaacgatc
     5341 aaatgtgcgt gctggtgcag atcgaaacgc gtgaggcaat gaagaactta ccgcagattc
     5401 tggacgtgga aggggtcgac ggcgtgttta tcggcccggc ggatctgagc gccgatatgg
     5461 gttatgccgg taatccacag cacccggaag tacaggccgc gattgagcag gcgatcgtgc
     5521 agatccgcga agcgggcaaa gcgccgggga tcctgatcgc caatgagcaa ctggcaaaac
     5581 gctatctgga actgggcgcg ctgtttgtcg ccgtcggcgt tgacaccacc ctgctcgccc
     5641 gcgccgctga agcgctggca gcacggtttg gcgcacaggc cacgaccgtt aagcccggcg
     5701 tgtattaatg ccggatgcgc tgcacttatc cggcctacac tcgcaccaaa ccgtaggccg
     5761 gataaggcgt ttacgccgca tccggcaaaa agccgtacca aatcgcggac cggataaggc
     5821 gtttgtgcgc atccgccata aaccttgtcg taccctacaa aaatcccatt agaggaagaa
     5881 aaatgagcga cacctcacct gccataccgg agagtatcga tccggcgaat cagcataaag
     5941 cgctgactgc cggacaacag gcggttatta agaagctgtt tcgccgcctg atcgtctttc
     6001 tgttcgtgct gtttatcttc tcgttccttg atcgcatcaa catcggcttt gccggactca
     6061 cgatgggacg cgacctcggt ctgagcgcca ccatgtttgg cctcgctacc accctgttct
     6121 atgccgctta tgtcatcttc ggcattccca gcaacattat gctgagtatt gtcggtgcgc
     6181 ggcgctggat cgccaccatc atggtgctct ggggcatcgc ctctactgcc accatgtttg
     6241 ccactggccc caccagcttg tacgtactgc gtatactggt tggcattacc gaagccggct
     6301 ttctgcctgg tattctgctg tatttaacct tctggttccc ggcctatttc cgcgcccgtg
     6361 ccaacgcctt gtttatggtt gcaatgccgg taacgacagc gttgggatcg ctcgtttccg
     6421 gctacatttt gtcgctggat ggcgtaatgg cattaaaagg ctggcagtgg ctgtttttgc
     6481 tggaaggctt cccgtcggta ttactcggcg tcatggtgtg gttctggctt gatgactcac
     6541 cggacaaagc taagtggctg acgaaagaag acaaaaaatg cctgcaagag atgatggata
     6601 acgatcgtct gacgctggtt cagccagagg gagccatcag ccaccacgcc atgcaacaac
     6661 gcagcatgtg gcgggagatc ttcactccgg tggtgatgat gtataccctg gcgtatttct
     6721 gcctgaccaa cacacttagt gcgatcagca tctggacacc gcagatcctg caaagcttta
     6781 atcagggcag cagtaatatc accatcggcc tgctggccgc cgtaccgcag atttgtacca
     6841 ttctcgggat gatctactgg agccgtcact cagatcgccg ccaggaacga aggcatcaca
     6901 ccgcccttcc ttatttgttc gctgccgcgg gttggttact ggcttcggca actgatcaca
     6961 acatgatcca gatgctgggg atcattatgg cttcgaccgg atcattcagc gcaatggcga
     7021 ttttctggac aacacctgat cagtccatca gcctgcgggc acgagcgatc ggtattgcgg
     7081 tgatcaacgc cactggcaac attggctcag cgttaagtcc gtttatgatc ggctggttga
     7141 aagatctgac cggcagcttt aacagtggat tgtggtttgt tgccgcgctg ctggtgattg
     7201 gtgcggggat tatctgggca attccaatgc agtcctcccg tccgcgagcg accccgtaag
     7261 gaacgacgat gtgtgaccgt cagattgcca atattgatat cagcaaagag tacgatgaaa
     7321 gcctgggcac ggacgatgtg cattatcagt ccttcgcccg catggcggca ttttttggcc
     7381 gccatatgct gccacatcgc cacgaacagt actttcagat gcatttcctc aatagcggac
     7441 agattgagct acagcttgac gatcatcgct actcggtgga agcgcccctg tttgtcctga
     7501 cgccgccgtc agtacctcat gcgtttatta cggagtctga cgccgacggt catgtgttga
     7561 cggtacggga agatctgatc tggcccctgc tggaagttct ttatccaggc actcgggaaa
     7621 ccttcggcct gccggggatt tgcctgtcac tggcagataa acccgacgaa ctggcggcgc
     7681 tggaacacta ttggcaactg atagagcggg aatcggtaga acaactgcct ggacgggaac
     7741 acaccctgac gttactggca caggcagtgt tcaccctact gctgcgtaac gcaaaactcg
     7801 acgaccatgc cgccagcgga atgcgcggag aattaaaact gttccagcgt tttcatatgc
     7861 tgattgaaag ccattttcat cagcactgga cagtaccgga ttacgctaac gaactgcata
     7921 tcaccgaatc acgcctcacg gacatctgcc gccgctttgc caaccgtccg ccaaaacggt
     7981 tgattttcga caggcagctg cgagaagcca agcggctgct gctgttttct gataacgccg
     8041 tgaacaatat tgcctggcaa ctcggtttta aggatccagc ttattttgcg cgctttttta
     8101 atcgcttagt cggttgctcg cccagtgctt atcgtgccaa aaaagtacct gtgacgtgaa
     8161 aatcccttag ccttaacggg aaaccaggca ccacctgcta ttcccctttc tccgcctgga
     8221 gcaaggggat ttagtaacgt caaaaatcgc taaaagcgac ctcgatcaca aaaccgtacc
     8281 cgacctgaaa agtaccagcc attgtcccaa aagtgtcttc ccgtaatcgc ccctgcaagc
     8341 ttcaattgaa taacaaaaac aacacacaaa attaacaatc gtattccgat taatactgta
     8401 gaggtcgaca tgaaaccaga agatttccgc gccagtaccc aacgtccgtt caccggggaa
     8461 gagtatctga aaagcctgca ggatggtcgc gagatctata tctatggcga gcgagtgaaa
     8521 gacgtcacta ctcatccggc atttcgtaat gcggcagcgt ctgttgccca actgtacgac
     8581 gcgctgcaca aaccggagat gcaggactct ctgtgctgga acaccgacac cggcagcggc
     8641 ggctataccc ataaattctt ccgcgtggcg aaaagtgccg acgacctgcg ccagcaacgt
     8701 gacgccatcg ctgagtggtc acgcctgagc tatggctgga tgggccgtac cccagactac
     8761 aaagctgctt tcggttgcgc actgggcgcg aatccgggct tttacggtca gttcgagcag
     8821 aacgcccgta actggtacac ccgtattcag gaaactggcc tctactttaa ccacgcgatt
     8881 gttaacccac cgatcgatcg tcatttgccg accgataaag tgaaagacgt ttacatcaag
     8941 ctggaaaaag agactgacgc cgggattatc gtcagcggtg cgaaagtggt tgccaccaac
     9001 tcggcgctga ctcactacaa catgattggc ttcggctcgg cacaagtgat gggcgaaaac
     9061 ccggacttcg cactgatgtt cgttgcgcca atggatgccg atggcgtgaa attaatctcc
     9121 cgcgcctctt atgagatggt cgcgggtgct accggctcgc cgtacgacta cccgctctcc
     9181 agccgctttg atgagaatga tgcgattctg gtgatggata acgtgctgat cccatgggaa
     9241 aacgtactga tctaccgcga ttttgatcgc tgccgtcgct ggacgatgga aggcggtttc
     9301 gcccgtatgt atcctctgca agcctgtgtg cgcctggcag tgaaactcga cttcattacg
     9361 gcactgctga aaaaatcact cgaatgtacc ggcaccctgg agttccgtgg tgtgcaggcc
     9421 gatctcggtg aagtggtggc gtggcgcaac accttctggg cattgagtga ctcgatgtgt
     9481 tctgaagcga cgccgtgggt caacggggct tatttaccgg atcatgccgc actacaaacc
     9541 tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa acattatcga acgcaacgtt
     9601 accagtggcc tgatctatct cccttccagt gcccgtgacc tgaacaatcc gcagatcgac
     9661 cagtatctgg cgaagtatgt gcgcggttcg aacgggatgg atcacgtcca gcgcatcaag
     9721 atcctcaaac tgatgtggga tgccattggc agcgagtttg gtggtcgtca cgaactgtat
     9781 gaaatcaact actccggtag ccaggatgag attcgcctgc agtgtctgcg ccaggcacaa
     9841 agctccggca atatggacaa gatgatggcg atggttgatc gctgcctgtc ggaatacgac
     9901 cagaacggct ggactgtgcc tcacctgcac aacaacgacg atatcaacat gctggataag
     9961 ctgctgaaat aacgcagcag gaggttaaga tgcaattaga tgaacaacgc ctgcgctttc
    10021 gtgacgcgat ggccagcctg tcggcagcgg taaatattat caccaccgag ggcgacgccg
    10081 gacaatgcgg gattacggca acggctgtct gctcggtcac ggatacacca ccgtcgctga
    10141 tggtgtgcat taacgccaac agtgcgatga acccggtttt tcagggcaac ggcaagttgt
    10201 gcgtcaacgt cctcaaccat gagcaggaac tgatggcacg ccacttcgcg ggcatgacag
    10261 gcatggcgat ggaagagcgt tttagcctct catgctggca aaaaggtccg ctggcgcagc
    10321 cggtgctgaa aggttcgctg gccagtcttg aaggtgagat ccgcgatgtg caggcaattg
    10381 gcacacatct ggtgtatctg gtggagatta aaaacatcat cctcagtgca gaaggtcacg
    10441 gacttatcta ctttaaacgc cgtttccatc cggtgatgct ggaaatggaa gctgcgattt
    10501 aagctccagc cccttcacgc attcgtcgaa ggggcatttt tattgtgatc tcaatcacat
    10561 atccacccga tgaataattc ttgcccaacc gctcaccgcc attcctgacc gcttacgtcc
    10621 ctaaaataac cactcagtta tttaccttac tttacgcgcg cgtaactctg gcaacatcac
    10681 tgcaggatag cggtcaattt acctcctcaa acgcaacgca aacctagaac ggcttcggcc
    10741 tatgattaac aaattactct atgaattttg gtttgcagga aggcagcaag tgagtgaatc
    10801 cccgggagct tacaacagta agtgacaggg gtgaacgaac gcaactgccg cacctgtaag
    10861 ctaaaagacg acgagtaaaa tgccaggttt tactatggat acgaaaaaac tattcaagca
    10921 cataccctgg gtgattctcg gaatcatcgg tgcattctgc ctcgcggtag ttgcattacg
    10981 tcggggggag cacgtaagcg ccctgtggat cgtggtcgcc tctgtgtcgg tgtatctggt
    11041 ggcgtatcgc tactacagtc tgtacatcgc ccagaaggtg atgaaactcg accccacgcg
    11101 cgcgacgcct gcggttatta acaacgacgg tctgaactac gttccgacca accgttacgt
    11161 gttgtttggt caccacttcg ccgctatcgc cggtgctggt ccgctggtcg gtccggttct
    11221 cgccgcacag atgggctacc tgcctggcac gctgtggctg ctggcggggg tagtactggc
    11281 cggtgcggtt caggacttta tggtgctgtt tatctcctct cgccgtaacg gcgcatctct
    11341 cggtgagatg ataaaagaag agatggggcc agttccgggg actatcgcgc tgtttggctg
    11401 tttcttaatc atgatcatca tcctcgccgt tctggcgctg attgtggtta aagccctggc
    11461 cgaaagtccg tggggtgtct tcaccgtttg ctcaaccgta ccgattgcgc tgtttatggg
    11521 tatctacatg cgctttatcc gtccggggcg tgtgggtgaa gtctctgtca ttggtatcgt
    11581 gctgctggtt gcctctatct acttcggtgg cgtgattgct cacgatccgt actggggtcc
    11641 ggcactgacc tttaaagaca ccaccattac cttcgcgctg attggctatg cgtttgtttc
    11701 cgcactgctg ccggtgtggc tgatcctcgc accgcgcgac tatctggcaa ccttcctgaa
    11761 aatcggcgtt atcgtcggcc tggcgctggg tatcgtggtg ctgaacccgg aactgaaaat
    11821 gcctgcgatg acccagtaca ttgacggtac tggcccgctg tggaaaggcg ctctgttccc
    11881 gttcctgttc atcaccatcg cctgtggtgc ggtatctggc ttccacgcgc tgatctcttc
    11941 cggtacgacg ccaaaactgc tggctaacga aaccgacgcg cgtttcattg gctacggcgc
    12001 aatgctgatg gagtccttcg tggcgattat ggcgctggtt gcagcgtcca tcatcgaacc
    12061 gggtctttac ttcgcgatga ataccccgcc tgctggcctt ggcatcacca tgcctaacct
    12121 gcatgaaatg ggtggcgaga acgcgccgat catcatggcg cagctgaaag acgttaccgc
    12181 acacgcggca gcgaccgtca gctcctgggg cttcgtgatt tcgccagagc agatcctgca
    12241 aaccgcgaaa gacattggtg agccttctgt cctgaaccgt gcaggtggtg cgccaacgct
    12301 ggcggtaggt atcgctcacg tgttccacaa agtgctgccg atggctgaca tgggcttctg
    12361 gtatcacttc ggtattctgt tcgaagccct gttcatcctg accgcgctgg atgcgggtac
    12421 tcgttctggc cgctttatgc tgcaagacct gctgggtaac ttcatcccgt tcctgaaaaa
    12481 aaccgactct ctggttgccg gtatcatcgg tactgcgggc tgtgtgggtc tgtggggcta
    12541 cctgctgtat cagggcgtgg tcgatccgct gggcggcgtt aagagcctgt ggccgctgtt
    12601 cggtatttcc aaccagatgc tggcagccgt agcgctggta ctgggcaccg ttgtgctgat
    12661 taagatgaag cgcacccaat acatctgggt aactgttgtt ccggctgtat ggctgcttat
    12721 ctgcaccacc tgggcgctgg gtctgaaact gttcagcacc aacccgcaga tggaaggctt
    12781 cttctacatg gcaagccagt acaaagagaa gattgctaac ggtactgacc tgacggcgca
    12841 gcagattgcc aacatgaacc acatcgttgt gaacaactac accaacgcag gcctgagtat
    12901 tctgttcctg attgtggtgt acagcatcat cttctacggt ttcaaaacct ggctgaatgt
    12961 gcgtaacagc gacaaacgta ctgacaaaga aacaccgtac gttccaatcc cggaaggcgg
    13021 cgtgaagatc tcttcgcacc actaaccgta tttagccccg cttcggcggg gctttgttct
    13081 tatcagagtg aactatgttt ggtaacttag gacaggcaaa aaaatatctc ggccaggcgg
    13141 cgaagatgtt gattggcatt ccagactacg acaactacgt cgagcatatg aagaccaacc
    13201 atcccgacaa gccgtacatg agctatgaag aattcttccg cgaacgccag aacgcacgct
    13261 acggcggcga tggtaaaggc ggtatgcgct gttgttaatg gagagagcat gaacccgatt
    13321 gcagttaccc tactcaccgg ttttttaggc gcgggcaaaa ccaccctgct gcgccatatt
    13381 cttaatgaac aacacggcta caagattgcc gtgattgaaa acgaattcgg cgaagtctcc
    13441 gttgatgatc aattgattgg tgatcgcgca acgcagatca aaacgctgac caacggctgc
    13501 atctgctgtt cgcgctccaa cgagctggag gacgcgctac tcgacctgct ggataatctc
    13561 gacaagggca atattcagtt cgatcgtctg gtcattgaat gcaccggcat ggccgatcct
    13621 ggcccgatta ttcaaacctt tttctcccat gagattttat gccagcgcta cctgctggac
    13681 ggcgtgattg cgctggtgga tgcggtacat gccgatgagc agatgaacca gttcaccatc
    13741 gcccagtccc aggtgggcta cgccgaccgt attctgctga ctaaaaccga cgtcgcaggc
    13801 gaagcagaaa aactgcgtga acgtctggca cgcatcaacg cccgtgcacc ggtctacacc
    13861 gtcacccacg gcgacatcga cctgggtctg ctgttcaaca ccaacggttt tatgctggaa
    13921 gaaaacgtcg tcagcaccaa accgcgtttc cattttatcg cggacaaaca aaacgatatt
    13981 tcgtcgattg tggtggaact ggattacccg gtagatatca gcgaagtttc ccgggtgatg
    14041 gaaaacctgc tgctggaatc ggcggataaa ctgctgcgtt acaaagggat gctgtggatt
    14101 gacggcgaac ctaaccgcct gctgttccag ggcgtccagc gcctctacag cgccgactgg
    14161 gacaggccat ggggcgatga aacaccgcat agcacgatgg tgtttatcgg gattcagttg
    14221 ccggaggatg agattcgggc ggcgtttgcg ggactcaaaa agtaaccagc cctccaacat
    14281 cacaatacct gagactttat caccctcata ttcctccaga atatgagggc tttcgggctt
    14341 aattatctct gaacgtgcaa cacttcctgc gactccttat gcgcctccac atcctgcttc
    14401 gttaaccaga gcgacttacg gccaaaattt tcgtacattt tcagctgatc ttcatagtgc
    14461 ttatcaactg ttccatcggg agcaataaac ccactctgac cgggtgcgac cacatcccag
    14521 gcaagcacag gacgatcgct tgtcgttggt gagaaaacaa tcatatcgtt ttctgttcca
    14581 cggttttgat actccgcctg atgacgcgtt tcttccgctg cggcctgcgg tacaccaaag
    14641 aaattatttg cccggaacgt taaggccatt gcaggtgttt tccagttact cacattattg
    14701 ccatagcgtt tggaaagagt ctcccaggta tcttccagcg cagccaacac aacctcctgc
    14761 tgtggtttcc cagcaaacag atcaaccgcc tgtgggattg gtgatttgtc tccctgcacc
    14821 gcctcataca aaatttttgc tccaacactt atattcagcg aaccagttgg gccgtcctgg
    14881 gttgtttcgt agccactggc gctgtaccac ttatcaaatg gcataggtac ggcagccact
    14941 acggtacgct tcaacatact ggtcagccaa acgttcagga tggcagagcc tggctgctgc
    15001 caggttttac catcatcatt aagcaaattg atgccatccc aacgtgttaa tgtttctacc
    15061 aactgacgac gcggatcgct ctgtgtcaaa ccagatgtcg ctgcttgcag agtaggtaaa
    15121 aaaagcctca ggttaagatc ctgacgactg gtttggcgaa taacatccca tgcctgatca
    15181 gcagttaagc gtggcttttg ctcaagcagt cggtcgatct ccgtaacgcg atctgcacca
    15241 ccccacaaaa aggcaaacag atctgaagcg ggataatctt tttggggaga attgttccag
    15301 ttagcaatat atcccgactg ggggttatac accttagggt tcatttcaaa aggcaatagc
    15361 cctttccagt cccattttcc cgtaccagga acgggtaatc gcggatcatg gcctgattga
    15421 cgatctggat aagcaccagt atgaacataa ccaatattgc cgtttacatc agcatagtac
    15481 cagttgatgg tcagtgcttg tttcgctgcc tgctgtgtcc actcctgcca atttttggcc
    15541 ttcatctgat gagtccaggc cagcaaagac gccacctctt taccatccca tgcgcgggat
    15601 ttagcgtaag ccgtttgtgt cgtctggtca gtttggagaa tgttgccatg caccgtacgc
    15661 cagacagtaa aggtctctgc ctgaccattt ttcaccgtaa tggtttcctc acggcttaac
    15721 attttcaccc acttaccatt atgcaagtag tagcctggtt tctctgccga cagccgttca
    15781 gcaaaaatat cgacatcatc gccgaaacct gccgttgatc cccaggaaat cacaccatta
    15841 tgaccaaaaa ccagcccagg ataggcaaat ggtgtattgc cagtgacatc ataaccagca
    15901 ccgtgcagac caataccata agtatacgca ggcgcatacc agccaaactg cggaccattt
    15961 accatgattg ctttcgcatc ctgggctttg cttttgccga tcacccacat attgctggtc
    16021 gttggatacc ccgccagacc attggcacca ccctgtgcaa attgtgcagc aatagtttcc
    16081 cggttcttcc ctgctgttaa cgccagcagt gcgccatccg ccccttttgc tggtcggtca
    16141 agcattggtg caggtaaatc gtagcgtggc aacagagctg ctgtttgcga gttttgctga
    16201 ttaaatttaa gtgggtagtt actctcttgt acggcaatag tggttggcgc tgatgggttt
    16261 accagccatt tcaactgatt aaataccgcc atgccttgtg atacaccata tttatctttt
    16321 aaagccgtta gcagtgccag attatcaatt tcgctagtgc tatcagagaa gcggtttgcc
    16381 atggtgccca caaatatcat cgcgacatca aacggttccc agcgcttagg agtaaagcca
    16441 aatgtattaa actgttttgg taagagcgtc tctggattgg tatttacctt atcaatccag
    16501 gcattcattc catcagcgta gccttgcaga atggacatat cctctgggga aagggcagca
    16561 atttgcgccc ggatagcatc cggccagtag ttacgacgga tatctttatc aaatttcaca
    16621 aaatctttgc caagcacttc cgcgacagtc ccttgagtac tgcgacgtgc catttccatc
    16681 tgaaaaaggc gatcttgtgc tactacatag ccatagccat aaaataggtg ccatgtatca
    16741 ttggcataaa tatgcggcat gccgtattca tcgcgaacaa tctttatctc acttgacgac
    16801 tgctcagcca gtgcaggtaa gctccaataa tacatcaggg aagcagtaac acagttcacg
    16861 atcatacgat ttctattttt cattgtatcc tctggcaggt gtataattag cgaattgata
    16921 ctagcaacga agcttcggtt atctatttgc agagaattgt ttcattatga actgtgagcg
    16981 cgataactaa tttttgatac aaaaatttca ttaatgtgat ctaaaacgaa tcgctttatt
    17041 caccgctgat gattttgttc tattcctttt tgtgacagat cccccggacg catagatgat
    17101 gacccccaga gagacagctc aggtcattat ctgtatgtac atcagaaaaa tatgttgtaa
    17161 acgactttta tggttggatt ttgtagcaat tttgaatgca gacaggcagc atttgccaac
    17221 acgccgccca tagttcactc cacatcctcc ctgcgtaacc caatatccct caaccgttca
    17281 tcactcatct gctgtaacac tcgtcgggtc tgcatttgcc gccgccaacg cctcaccgca
    17341 tgccagagtt gcacaagtct gataaacggc gttttggctt tgttttcgtg aaattccatc
    17401 tgctgcctcc tcatctttca gaggcttcat catccgcctt ctggcagttc acaatacaga
    17461 tgcaaatatt agttttattt aacatacaga caggttaaaa cagcatctgc atccggtttt
    17521 ttagcctcat ctgtaccggt ttttctttct gtatgggcca aataaggaat acagcatgac
    17581 gcgttatcaa catctggcga ctctacttgc cgagcggatt gagcaagggc tgtatcgtca
    17641 cggggagaaa ttgccgtcgg tgcgcagctt aagtcaggag cacggcgtca gcatcagcac
    17701 cgtgcaacag gcgtatcaga cgctggaaac gatgaggctc atcactccgc agccgcgttc
    17761 gggttatttt gtcgcacaac gtaaagccca gccgcctgta ccgccgatga cgcgtccggt
    17821 gcagcgcccg gtggaaatta cccagtggga tcaggtgctg gatatgctgg aggcgcatag
    17881 cgacaattcc attgttccgt taagcaaaag cacgccggat gtcgaaacgc ccagcctgaa
    17941 accgctgtgg cgtgagctaa gccgggtggt gcagcataat ctgcaaaccg ttctcggtta
    18001 tgacttgtta gccggtcagc gagtgttgcg ggagcagatt gcccgcctga tgctcgacag
    18061 cggctcggtg gtcaccgccg atgacatcat catcaccagc ggctgccata actcgatgtc
    18121 gctggcgtta atggcggtgt gtaaaccggg cgatattgtc gcggtcgaat ctccctgtta
    18181 ttacggttcg atgcagatgc tgcgaggcat gggcgtgaaa gtgattgaaa tcccaaccga
    18241 tccagaaact ggcatcagcg ttgaagcact ggaactggcg ctggaacagt ggccgattaa
    18301 aggcatcatt ctggtgccaa actgtaataa tccgctggga tttattatgc cggacgcacg
    18361 caaacgggcc gttctctctc tcgctcagcg tcatgatatt gtgatttttg aagatgatgt
    18421 ctacggcgaa ctggcaacgg agtatccgcg cccgcggacc atccattcat gggatatcga
    18481 cgggcgagtg ttgttgtgca gctcgttcag taaaagtatt gctccaggcc tgcgcgtggg
    18541 ttgggtcgca ccggggcgtt atcacgataa actgatgcat atgaaatacg ccatcagcag
    18601 ctttaatgtg ccgtccacgc aaatggcggc ggcaacgttt gtgctggaag gtcactatca
    18661 tcgccatatc cggcggatgc ggcagagcta tcagcgcaat ttggcgcttt atacctgctg
    18721 gatacgggaa tattttccct gcgaaatctg tattacgcgc ccgaaaggcg gatttttact
    18781 gtggatagaa ttgcctgaac aggtcgatat ggtctgcgtt gcgcggcagc tgtgccgcat
    18841 gaatatccag gtggcagcag gctcgatttt ctcggcttcc ggcaaatacc gtaattgtct
    18901 acgcatcaac tgcgctttgc cgctcagcga aacctatcgc gaagcactaa agcaaattgg
    18961 cgaggccgtg tatcgggcaa tggaataaat tcgaattagt ggcgaagcac ctgatttcct
    19021 ctcacgaatc gcctgtaact cgttgtatct ttgcagggtc atcgccacgt tccagcatgg
    19081 cttaggcaaa gtgtgcttcg ttcataccgg atgcggcgtg aacgccttat ccggcctaca
    19141 aaccgcgcaa attcaataga ttgcagagga aatgtaggtc tgataagcgt agcgcatcag
    19201 gcagctttgc gtttgtcatc cgtctctgat tagtattcga tccatggatc gcgcttgatt
    19261 tcaggcaagg gctgctctaa tgcctgcatt tgctctgtcg aaagtccggt aatcttttgt
    19321 atccattcag gatctgcccc attctgcaac aacaatctaa ggaggcgttg ttccccttct
    19381 tctttcccct tttccatccc caacagccat ccatcattat aaattcgctc tgcaatcgtc
    19441 attattcgct ccctgtgttg tggcattcga cgggtaagtt cgctgataaa cttcttaaaa
    19501 cgcgcttcat cgccagtcag taaaatgtaa tttaacagcg cagttatctg gctgtcatta
    19561 gcacactctg taaccagtaa tattaccagt tgatcgataa gccccatcag atcgcgctgg
    19621 cgaatatgct tttggatcaa ctccaacagg gcgactctgc ggtgctgcac aatctcgtcg
    19681 tctggcacga cagtgacatc caccagcggg aacgctgcgg tataaagctt ccgtgcggtg
    19741 gtcgggtcag caaattcgtc cagccagcac agggaccagg gataaggact acggctaccg
    19801 tgataaaaca gcatcgggat gaccagcggt agcggccggc gtttatcatg ctcgatatgg
    19861 cgctgcatca ccgccatgga atatcgcatc aggcgaaagg ccatatggat atcctcgcgg
    19921 ctctgatgtt caatcactac gtaaatataa ccatcacctt cacgggtctt taccgaccac
    19981 agaatatcgg agtgtagcgc ccgcaatttt tcatcgacga agctggcgga ttccagtttt
    20041 aagctgtcga gatcgcacag ttcacgtaaa tctttgggta agtggatctc cataaaatcc
    20101 cgcgcggtgt cagggtgcgt gagaaaggat ttaaataatg cgtcatgcgg cgtgctggtt
    20161 gtgaagtttg tcatggcgat ccgtcacctg tagataagat ggcccgactg tagcgactaa
    20221 caaaaccatt ctcacccggc aatttctcgt tccccgaggc gctttccagg ctatttgtgt
    20281 tcctctgcaa acgatacatt tttccggaaa caggctcgcg ttttacattt cctgtacccg
    20341 gcagatattc ccctttccac cgcgctcttt tgattaaccg caaattttaa ttacacttaa
    20401 ggtgtatatt ttctatgcaa cccatcaatt aaagaggtgt aatgtgctga tgactatttg
    20461 taatcgttat acattctgac ctggagtcag aaggtatttc tctgtctgtg tgttcacagg
    20521 cagtgtggtt gattacatga aattcagtac atttgtcctt ctcgcctgcc tttcgtcatt
    20581 accgacggta ttgaatttcg ttttccccgt tggggttctc cggacaagga gttgtttgtt
    20641 atgccacgtt tttttgcccg ccatgccgct acgctgtttt tcccgatggc gttgattttg
    20701 tatgactttg ccgcctatct ttcgacggat ctgatccagc cggggatcat taatgtggtc
    20761 cgtgatttta atgccgatgt cagtctggct ccggctgccg tcagtctcta tctcgcgggg
    20821 ggtatggcgt tacagtggct gctggggccg ctttccgaca ggattggccg caggccggtg
    20881 ctgattaccg gggcgctaat atttaccctt gcctgcgctg cgacaatgtt cacaacgtct
    20941 atgacacagt ttcttatcgc gcgtgcaatt cagggcacca gtatctgttt tattgctacc
    21001 gttggttatg tcacggtgca ggaggcgttc ggacagacaa aagggattaa gttgatggcg
    21061 attatcacct ccatcgtact gattgcgccg attattggcc cgctttccgg cgcagctctg
    21121 atgcactttg tgcactggaa agtccttttt gccatcattg cggttatggg gtttatctca
    21181 tttgttggct tactgctggc gatgccagag acggtgaagc gcggcgcggt tccatttagc
    21241 gccaaaagcg tcttgcgcga ttttcgtaat gtcttttgca accggctgtt cctctttggc
    21301 gcagcaacca tctctttaag ctatatcccg atgatgagtt gggtggctgt ctcgccggtg
    21361 atcctgattg acgcaggcgg cttaacaact tcgcagttcg cctggacaca ggttccggtg
    21421 ttcggcgcgg tgattgtcgc gaatgccatc gtggcgcgtt tcgttaaaga tccgaccgaa
    21481 ccgcgcttta tctggcgtgc tgtacccatt caactggtcg gcctcgcgct gttgattatc
    21541 ggcaatctgc tgtcaccgca cgtctggctg tggtcggtgc tgggcaccag tttgtatgct
    21601 ttcgggattg gtttgatctt cccgacctta ttccgcttta cgcttttttc caataactta
    21661 ccgaaaggaa ccgtctccgc atcgctgaat atggtgatcc tgatggtgat gtctgtctcg
    21721 gtcgaaatcg gccgctggct gtggtttaac ggcggtcgct tgccgtttca tctgttagcc
    21781 gtcgtggcgg gcgttatcgt cgttttcacc ctggcaggat tgctcaatcg cgtgcgccaa
    21841 catcaggctg ccgagctggc ggaggagcgg tgatttttgc gcgatcctgc cgtcaggctc
    21901 tattcttaac gttatgaata aactcattga actcagacgc gccaaaatgt tggcgctctc
    21961 tttactgctt atcgccgctg ctacctttgt cgttacgctg tttttgccgc ccaatttttg
    22021 ggtgagcggc gtgaaggcga ttgctgaagc ggcgatggtc ggcgcgctgg cggactggtt
    22081 tgcggtggtg gcgctgtttc gccgcgtgcc gattccgatc atttctcgtc atacggcgat
    22141 tatcccacgt aataaagacc ggattggcga aaatctcggc cagttcgtgc aggaaaaatt
    22201 tctcgatacc cagtcgctgg tggcattgat tcgacgtcac gaaccagcgt tgttgattgg
    22261 caactggttt agtcagccag aaaacgcccg ccgcgttggt cagcatctgt tgcagatcat
    22321 gagcggtttt cttgaactga ccgatgatgc gcgtattcag cgcctgctta agcgcgcggt
    22381 ccatcgggcg attgataagg tagatctttc cggcaccagt gcgttgatgc tggagagtat
    22441 gaccaaaaac gatcgtcatc aggtgctgct ggatacgctg atcgcacagt tgatcgccct
    22501 tctccagcgc gataaatcgc gcaagttaat cgcccagcaa attgttcgct ggctggagag
    22561 cgagcatcca ctaaaagcca aaattctccc caccgaatgg ctgggcgaac atagcgcgga
    22621 gttggtttct gacgcggtga attctttgct tgatgatatc agccgcgatc gtgcgcatca
    22681 gatccgccat gcgtttgatc gcgccacttt tgccctgatc gacaagttga aaaacgatcc
    22741 tgaaatggca gcgcgagctg atgccgtaaa aagctatctg aaagaagatg aagcttttaa
    22801 ccgctatctc agtgaattgt ggggggattt acgggaatgg ctgaaagcgg atatcaacag
    22861 tgaagattct cgtgtgaaag aacgtatcgc gcgggcgggt caatggtttg gcgaaacgtt
    22921 aattgccgat gatgccttgc gggcgtcgtt aaatggtcat ctggaacaag ccgcgcaccg
    22981 cgtcgcgcct gagttttccg cattcctgac gcgccatatc agcgatacgg taaaaagctg
    23041 ggatgcgcgg gatatgtcgc ggcaaataga gttaaatatc ggcaaagatc tgcagtttat
    23101 ccgtgtcaac ggtacgctgg ttggcggttg tattgggcta attttatatt tgttgtcgca
    23161 gctcccggcc ttgttccccc tcggcaattt ttagaaatcc atgaaaaact aagtagctaa
    23221 ccgcgagaga gatcaaataa accacactaa aagaatggta ataatgtggc ctcttttcat
    23281 tatctcccgt ggtacgggga aggaaaatca tgtcacttgt caccgatcta cccgccattt
    23341 tcgatcagtt ctctgaagct cgccagaaag gctttctcac cgtcatggat ctcaaggagc
    23401 gcggcattcc gctggttggc acttactgca cctttatgcc gcaagagatc ccgatggcag
    23461 ccggtgcggt tgtggtttcg ctctgttcca cctctgatga aaccattgaa gaagcggaga
    23521 aagatctgcc gcgcaacctc tgcccgctga ttaaaagcag ctacggcttc ggcaaaaccg
    23581 ataaatgccc ctacttctac ttttctgatc tggtggtcgg tgaaaccacc tgcgacggca
    23641 aaaagaaaat gtatgaatac atggcggagt ttaagcccgt tcatgtgatg cagctgccga
    23701 acagcgttaa ggacgatacc tcgcgtgcgt tatggaaagc cgagatgctg cgcttgcaaa
    23761 aaacggtaga agaacgtttt gggcacgaga ttagcgaaga tgctctgcgc gatgccattg
    23821 ctctgaaaaa ccgcgaacgt cgcgcactgg ctaattttta tcatcttggg cagttaaatc
    23881 ctccggcgct tagcggcagc gacattctga aagtggttta cggcgcaaca ttccggttcg
    23941 ataaagaggc gttgatcaat gaactggacg cgatgacagc ccgcgttcgt cagcagtggg
    24001 aagaaggcca gcgactggac ccgcgtccac gcattttaat caccggctgc ccgattggcg
    24061 gcgcagcaga aaaagtggtg cgcgcgattg aagagaatgg cggctgggtt gtcggttatg
    24121 aaaactgcac cggggcgaaa gcgaccgagc aatgcgtggc agaaacgggc gatgtctacg
    24181 acgcactggc ggataaatat ctggcgattg gctgctcctg tgtttcgccg aacgatcagc
    24241 gcctgcaaat gctcagccag atggtggaag aatatcaggt cgatggcgta gttgatgtga
    24301 ttttgcaggc gtgccatacc tacgcggtgg aatcgctggc gattaaacgt catgtgcgtc
    24361 agcagcacaa cattccttat atcgctattg aaacagacta ctccacctca gatgtcgggc
    24421 agctcagtac ccgtgtcgcg gcctttattg agatgctgta aggagtggca gtgacatatt
    24481 cgattggcat tgattctggt tctaccgcca ccaaagggat cttactggca gacggcgtga
    24541 ttacgcgccg tttcctcgtt ccaacaccct ttcgtccggc aacagcaatt actgaagcct
    24601 gggaaactct gcgcgaaggg ttagagacaa cgccgtttct gacgctcacc ggctatggac
    24661 ggcaactggt ggattttgcc gataaacaag tgacggaaat ctcctgtcat gggctgggcg
    24721 cacggtttct tgcgccagca acgcgcgcgg taatcgacat cggtggtcag gacagcaaag
    24781 tgattcagct tgatgatgac ggtaacctgt gcgatttcct gatgaatgac aaatgcgcgg
    24841 cgggcaccgg gcgtttcctg gaggtgatct cgcgcacgct tggcaccagc gtcgagcaac
    24901 tcgacagcat taccgaaaat gtcacgccgc acgccatcac gagtatgtgc acagtgtttg
    24961 ccgaatcaga agtgatcagc ctgcgctcag cgggcgtcgc gccagaagcg attctcgccg
    25021 gagtgattaa cgcgatggcg cggaggagtg ccaatttcat tgctcgtctc tcctgtgaag
    25081 cgccgattct gtttactggt ggcgttagtc attgccagac gtttgcccgg atgctggaat
    25141 ctcatctggg aatgccggta aatacccatc ctgatgcgca atttgctggc gcaattggcg
    25201 cggcggtaat tggtcaacga gtgaggacac gccgatgaaa gagtttttat ttttatttca
    25261 ctccacagtc ggcgtcatac aaacccgcaa agcgttgcag gcagcgggca tgacttttcg
    25321 cgtcagcgat attccccgtg atttacgcgg cggctgcggg ttatgcattt ggctgacctg
    25381 tccccccggc gaggaaatac aatgggtgat ccctgggctg actgagtcaa tttattgtca
    25441 gcaggatggt gtctggcgct gcattgcaca ttaccgggtt tctccgcgtt aaggattcat
    25501 taaggtcgct acgtatactt ctcgatatct tattgatttg ccttaaaggg attaccgtga
    25561 caaaaagtaa ttcgttaagt aaacgcatct ccgttattgt gattcttttt gccattgtgg
    25621 cagtgtgtac tttcttcgtg caatcttgcg cccgtaaaag caatcatgca gcatcatttc
    25681 agaattacca cgccaccatt gacggcaaag aaattgcagg cataacgaat aatatctctt
    25741 cgctgacctg gtcggcacaa agcaatactc tttttagcac tatcaacaaa cctgccgcca
    25801 ttgttgaaat gaccaccaat ggtgatttta tccgcacaat tcctctggat tttgttaagg
    25861 atctcgagac gatcgaatac attggcgata atcagtttgt cattagcgat gagcgcgatt
    25921 acgccattta tgtgatttca ctgaccccaa actcggaagt aaaaatcctc aaaaaaataa
    25981 aaatcccgct gcaagactcc ccaaccaatt gcggttttga agggttggct tattctcgcc
    26041 aggatcacac tttctggttt tttaaagaga aaaatccgat tgaggtctac aaagttaacg
    26101 ggctgttaag tagcaatgaa ttacacatca gcaaagacga ggcactacaa cggcaattta
    26161 ctttagatga tgtgtccggc gcagaattta accagcaaaa aaatacgctt ctggtgctgt
    26221 ctcatgaatc tcgcgcactc caggaagtaa ccctggttgg cgaggttatc ggtgaaatat
    26281 cactaaccaa aggtagtcga ggtttgtcgc ataacatcaa acaggccgaa gggattgcga
    26341 tggatgcctc tggtaatatt tatatcgtta gcgaaccaaa tcgtttttat cgctttaccc
    26401 ctaaatcatc gcattaacag gctggcatat ttcacctttt tgttattaac ttgttaacaa
    26461 caaaaatgac atacaaggtt aaacatgcct tcgtcctcgc atcccgtaga acgcttttct
    26521 ttcagcaccg cgcttttcgg gatgctggtt ctgaccctag gtatgggatt aggtcgcttc
    26581 ctttatacgc ctatgttgcc cgtcatgatg gcggaagggg cgttttcatt tagccagctc
    26641 tcgtggattg ccagcggcaa ctatgcgggg tatctggctg gcagtctgct attttcgttt
    26701 ggcgcatttc accagccgtc gcgcctacgc ccatttctgt tggtttctgc cctggcgagc
    26761 gggttgttga tcctcgcaat ggcatggttg ccgccattta ttctggtgtt attgattcgc
    26821 ttcctggcgg gtgtcgccag cgccggtatg ctgatttttg gttcgacgct gattatgcag
    26881 cacacgcgcc atccttttgt gctggcagct ttgttttctg gcgttggcat tggcattgca
    26941 ctgggcaacg aatatgttct ggcaggcctg cattttgcct tctcctcgca aacgttatgg
    27001 caaggcgccg gggcgctttc tggcatgatg ctgattgcac ttacgctttt aatgccctcg
    27061 aaaaaacacg ccatcgcacc aatgccattg gcaaaaacgg agcaacagat aatgaactgg
    27121 tggttactgg ctattctgta cggcctggcg ggatttggtt atatcatcgt cgcgactttc
    27181 ctgccgctca tggcgaaaga tgcaggctca ccattgttaa ccgcccatct ctggacgtta
    27241 gtcggcttat caattgtgcc aggctgcttt ggctggctat gggcagcaaa acgttggggg
    27301 gctttgcctt gcctgacggc gaatttgctg gtgcaggcta tcagtgtgct gcttactctc
    27361 gccagcgact ctcctctctt gcttatcatt agtagtatcg gttttggcgg tacctttatg
    27421 ggcacgacat ctctggtgat gactatcgcc cgccaactga gcgttccggg aaatctcaat
    27481 cttttaggct ttgtgacgct catttacggc attggacaaa tcctcggccc ggcgctgacc
    27541 agtatgctcg gcaatggaac atcggcgctt gccggcgcca ctctctgcgg cgcggcggcg
    27601 ttgtttattg ctgcgcttat cagcacagta caattattta agctgcaagt ggtcacttca
    27661 tgaggagtgt ttcccttacc actcaagaaa ctcaacagca accgttgatg tcagccagac
    27721 cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc atttcctgtg ctttgacggt
    27781 taagataacc ggcgatccgt gtcttgctcc cactttgcgt gccgttgctt catcagcaga
    27841 gagatgtacg taatgacgct cccctgcaat cagcccctgt ttttttatct cgtcaaggaa
    27901 acggcttgcc gtaccgtggt agagaaactg tggcggtgtt ttttcggcaa aggaaatggc
    27961 gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac ctaccatcac tggaataact
    28021 aaaacgtttt ttatcactgg tagcgaccac ggtatccagc aatgcacggg ttaaccgctt
    28081 gcccgctttt tgggcacaaa gtatgagctt atcgatatcc gcccatcctt cacggtccag
    28141 tacgatacct atcgcctccg gtttgtgtcg gaggacaaaa cttaaaaact tgctcgtatc
    28201 cgccagctct ttttcgttgt actttgccat cgcgttttcg tccctggatt ttctgttttt
    28261 atttactgat gtcattgtca ctgggtttat gaattttctg caactcaatc ctgcgcagag
    28321 ccttttccac acagcataat ttttccgcta tgcaaaaaac gcatagccag aaacatactt
    28381 gcattttccc catacccaca tggctgataa ggtttttccg ctccaggaat tttcctggct
    28441 caagcaacac acaaacacaa aaaaacaatg gggattgtta tgacacaaca aggggatgct
    28501 gtagccgggg agctggcgac agaaaaagtg ggcattaaag ggtatctggc tttttttctc
    28561 accataatct ttttttccgg tgttttttcc ggaacagaca gctggtggcg cgtttttgat
    28621 ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg cgaacggagc caccacctca
    28681 tttcgcgggg cgggtggcgc gggggctaaa gatggctttc tctttgcgct ggagctggca
    28741 ccgtcagtca ttctctctct ggggattatt tcaattaccg atggtcttgg tggattacgc
    28801 gccgcgcagc agttaatgac gcctgtgcta aaaccgttgt tgggcattcc agggatctgc
    28861 tcgctggcgc tgatcgctaa cctgcaaaac actgatgccg ccgccggtat gaccaaagag
    28921 ctggcgcagg aaggggaaat taccgaacgc gataaagtga ttttcgccgc atatcaaacc
    28981 agcggtagcg ccatcatcac caactacttc tcttccggcg tcgcggtgtt tgcctttctg
    29041 ggcacgtccg taatcgtacc tttagccgtc atcctggtgt ttaaatttgt cggagccaac
    29101 attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc caacgcaagg agcgcaagca
    29161 tgacaactca ggtccgcaaa aacgtcatgg atatgtttat cgatggcgcg cgccgtggct
    29221 ttactatcgc cacgaccaac ctactgccaa acgtggtgat ggcgtttgtc atcattcagg
    29281 cgctgaaaat taccggcctg ctcgactggg tggggcatat ctgtgaaccc gtaatggcgc
    29341 tgtggggatt acccggtgaa gcggcaaccg tccttttagc cgcattaatg agtatgggcg
    29401 gcgcagtcgg tgttgccgcc agtctggcga ccgccggcgc attaaccggt cacgatgtca
    29461 ccgttttact gcccgcgatg tatttaatgg gcaacccggt gcaaaatgtc ggtcgctgcc
    29521 tggggacagc cgaagtgaat gccaaatatt acccgcatat cattacggtc tgcgtgatta
    29581 acgcattgct gtcgatctgg gttatgcagc ttattgttta acaaggagtt accatgattg
    29641 attataccgc agccggtttt accctgctgc agggagcgca tttgtatgcg ccggaagatc
    29701 ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat cgccgttgcc agcaatatcc
    29761 cttctgacat tgtaccggac tgcacggttg tcgatctcag cgggcagatc ctctgtcctg
    29821 gttttattga tcaacacgtc catttgattg gtggtggcgg cgaagcaggt cccacgacgc
    29881 gcacgccaga agtggcgcta agtcgcctga cggaagcggg cgtcacgtca gtggttggtc
    29941 tgctgggcac cgactctatc tctcgccacc cggaatccct gctcgccaag acccgtgcgc
    30001 tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc ttatcatgtc ccttcccgca
    30061 ccattacagg ttccgtggaa aaagacgtgg cgattatcga tcgtgtgatt ggcgtgaaat
    30121 gcgccatctc tgatcaccgt tctgccgcac cggacgttta tcacctggct aatatggcgg
    30181 cggaatcccg cgttggcggt ttgctcggcg gtaaacctgg cgtcaccgtg ttccacatgg
    30241 gcgacagcaa aaaggcgtta cagcctgtct atgacctgct ggaaaactgc gatgtgccga
    30301 tcagcaagtt gctgccgacc cacgttaacc gcaacgttcc gttgtttgag caggcgctgg
    30361 agttcgcgcg caaaggcggc actatcgata tcaccagcag cattgacgaa ccggtcgccc
    30421 ctgccgaagg tattgcccgc gccgttcagg cgggtattcc gctggcacgc gtcaccctca
    30481 gctccgacgg caacggtagc cagccgttct tcgatgacga agggaattta acccatatcg
    30541 gtgttgccgg ttttgaaacg ttgctggaaa ccgtgcaggt gctggtcaaa gactatgatt
    30601 tcagtatcag cgatgccctg cgcccgctca ccagtagcgt agccggtttc cttaacctga
    30661 ccgggaaagg cgaaattctg ccaggcaatg atgctgactt gctggtcatg acgccagaac
    30721 tgcgcattga gcaggtatac gctcgcggca aactgatggt caaagacggc aaagcctgcg
    30781 tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg cccgaatgga cgtagaatgc
    30841 ccgcaagggc ggctgacaga gtaaaacgta atggatgact gtggtgcgat tttgcataat
    30901 attgaaacca aatggcttta tgattttctg accctggaaa aatgccgcaa tttttcccag
    30961 gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc gccgcatccg tgcgctggaa
    31021 caggcgattg gtgttgaatt gtttaaccgc caggtaacgc cgctgcaact ctcagaacaa
    31081 gggaaaatct tccattcgca gatccgccat ctgttgcaac agttagagag caatctggca
    31141 gagctgcgtg gcggcagcga ttacgcgcaa cgaaaaatca agatagccgc tgcacactct
    31201 ctttccctcg ggctgttacc gtccattatc agccagatgc cgccgctctt tacctgggca
    31261 attgaagcta ttgatgtcga tgaagcggtc gataaactgc gtgaagggca aagtgactgt
    31321 attttttcct ttcacgacga agatttgctg gaagcgccgt ttgaccacat tcgcttattt
    31381 gaatctcaat tgttccctgt ctgcgccagt gacgaacacg gagaagcact ttttaacctc
    31441 gcgcagccac actttccgtt actgaattac agccgcaact cctacatggg gcgattgatt
    31501 aatcgcaccc tgacgcgcca cagtgagtta agtttcagca ccttttttgt ctcttcgatg
    31561 agcgagcttt taaagcaggt tgccctcgac ggctgtggga ttgcctggct gccggagtac
    31621 gccatccaac aagaaattcg cagcgggaag ctcgttgtac tgaatcggga cgaactggtg
    31681 atcccgattc aggcctatgc ataccgaatg aatacccgca tgaatccagt tgccgaacgc
    31741 ttctggcgtg aactgcgcga gctggagatt gtgcttagct gacattctcc agcatcgcac
    31801 tacaccaacc gccattatcc cagaccagat caaacgttag cggttcaccg tcgtggtaca
    31861 gcattatctc aaggctgatc gccagagtgt ggcagtacgc ttctttaaaa ttaacctgca
    31921 acgccgcaat ccgtggctca tgaaataaca gtgcagaacg cagctgacgg caataccagc
    31981 tctggagatg tttgtcgctc tggttatgca cttgccactc tggcatgcct gaaacaagta
    32041 atgacggtaa tgccgaatga ggagaattaa aaagagtgag gagatcgctg cataccgatt
    32101 tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa aacagcctga agtgattggc
    32161 gcatcatttt gcgcactcct gacgtttagc aacatatgcg gcttgccctg aacaccgggc
    32221 accactgtat tgatgctcat tttccatagc cattcccttt tgttcattac atccatgtca
    32281 gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac cttacttgcc tatgaatatc
    32341 tactgacttt tcaggaaaaa agtcatgatt tgataaaaat catcaaactc aaaaaaaaac
    32401 cgctagtata ttctgaatat tccgaatagt tacgtagatg aaaatacttg tcaccgctgc
    32461 tacttaattc cggtgggaaa ttatttattt ccgcataatc agccatgtat tatttgaaac
    32521 aattagtttg taaataatat aaattgataa catatcaaat acatcaaaaa taccattcac
    32581 aggcattatt aatatagaca tcaatatagc aacatcaaa
//
LOCUS       DAZGSP010000053        32242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZGSP010000053 DAZGSP010000000
VERSION     DAZGSP010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.053"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            182..481
                     /locus_tag="ACHK74_002721"
     CDS             182..481
                     /locus_tag="ACHK74_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221510.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSYFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(528..1415)
                     /gene="rarD"
                     /locus_tag="ACHK74_002722"
     CDS             complement(528..1415)
                     /gene="rarD"
                     /locus_tag="ACHK74_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFP0221511.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1467..1934)
                     /gene="yigI"
                     /locus_tag="ACHK74_002723"
     CDS             complement(1467..1934)
                     /gene="yigI"
                     /locus_tag="ACHK74_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFP0221512.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2099..2968
                     /gene="pldA"
                     /locus_tag="ACHK74_002724"
     CDS             2099..2968
                     /gene="pldA"
                     /locus_tag="ACHK74_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFP0221513.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3101..4930
                     /gene="recQ"
                     /locus_tag="ACHK74_002725"
     CDS             3101..4930
                     /gene="recQ"
                     /locus_tag="ACHK74_002725"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFP0221514.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQINSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4994..5614
                     /gene="rhtC"
                     /locus_tag="ACHK74_002726"
     CDS             4994..5614
                     /gene="rhtC"
                     /locus_tag="ACHK74_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFP0221515.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5676..6296)
                     /gene="rhtB"
                     /locus_tag="ACHK74_002727"
     CDS             complement(5676..6296)
                     /gene="rhtB"
                     /locus_tag="ACHK74_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFP0221516.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6408..7430
                     /gene="pldB"
                     /locus_tag="ACHK74_002728"
     CDS             6408..7430
                     /gene="pldB"
                     /locus_tag="ACHK74_002728"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFP0221517.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7438..8238
                     /gene="yigL"
                     /locus_tag="ACHK74_002729"
     CDS             7438..8238
                     /gene="yigL"
                     /locus_tag="ACHK74_002729"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFP0221518.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8314..9213
                     /gene="bioP"
                     /locus_tag="ACHK74_002730"
     CDS             8314..9213
                     /gene="bioP"
                     /locus_tag="ACHK74_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFP0221519.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(9101..10054)
                     /gene="metR"
                     /locus_tag="ACHK74_002731"
     CDS             complement(9101..10054)
                     /gene="metR"
                     /locus_tag="ACHK74_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFP0221520.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10172..12433
                     /gene="metE"
                     /locus_tag="ACHK74_002732"
     CDS             10172..12433
                     /gene="metE"
                     /locus_tag="ACHK74_002732"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFP0221521.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12473..13288)
                     /gene="ysgA"
                     /locus_tag="ACHK74_002733"
     CDS             complement(12473..13288)
                     /gene="ysgA"
                     /locus_tag="ACHK74_002733"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFP0221522.1"
                     /translation="MATTQQSGFTPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGQLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13550..14311
                     /gene="udp"
                     /locus_tag="ACHK74_002734"
     CDS             13550..14311
                     /gene="udp"
                     /locus_tag="ACHK74_002734"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFP0221523.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14452..15879
                     /gene="rmuC"
                     /locus_tag="ACHK74_002735"
     CDS             14452..15879
                     /gene="rmuC"
                     /locus_tag="ACHK74_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFP0221524.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQYAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFSLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15974..16729
                     /gene="ubiE"
                     /locus_tag="ACHK74_002736"
     CDS             15974..16729
                     /gene="ubiE"
                     /locus_tag="ACHK74_002736"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFP0221525.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16743..17348
                     /gene="ubiJ"
                     /locus_tag="ACHK74_002737"
     CDS             16743..17348
                     /gene="ubiJ"
                     /locus_tag="ACHK74_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFP0221526.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17345..18985
                     /gene="ubiB"
                     /locus_tag="ACHK74_002738"
     CDS             17345..18985
                     /gene="ubiB"
                     /locus_tag="ACHK74_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFP0221527.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            19064..19333
                     /gene="tatA"
                     /locus_tag="ACHK74_002739"
     CDS             19064..19333
                     /gene="tatA"
                     /locus_tag="ACHK74_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFP0221528.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19337..19852
                     /gene="tatB"
                     /locus_tag="ACHK74_002740"
     CDS             19337..19852
                     /gene="tatB"
                     /locus_tag="ACHK74_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFP0221529.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19855..20631
                     /gene="tatC"
                     /locus_tag="ACHK74_002741"
     CDS             19855..20631
                     /gene="tatC"
                     /locus_tag="ACHK74_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFP0221530.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20673..21455
                     /gene="tatD"
                     /locus_tag="ACHK74_002742"
     CDS             20673..21455
                     /gene="tatD"
                     /locus_tag="ACHK74_002742"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFP0221531.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21452..21940)
                     /gene="rfaH"
                     /locus_tag="ACHK74_002743"
     CDS             complement(21452..21940)
                     /gene="rfaH"
                     /locus_tag="ACHK74_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFP0221532.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22107..23600
                     /gene="ubiD"
                     /locus_tag="ACHK74_002744"
     CDS             22107..23600
                     /gene="ubiD"
                     /locus_tag="ACHK74_002744"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFP0221533.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23646..24347
                     /gene="fre"
                     /locus_tag="ACHK74_002745"
     CDS             23646..24347
                     /gene="fre"
                     /locus_tag="ACHK74_002745"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFP0221534.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24539..25702)
                     /gene="fadA"
                     /locus_tag="ACHK74_002746"
     CDS             complement(24539..25702)
                     /gene="fadA"
                     /locus_tag="ACHK74_002746"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFP0221535.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25712..27901)
                     /gene="fadB"
                     /locus_tag="ACHK74_002747"
     CDS             complement(25712..27901)
                     /gene="fadB"
                     /locus_tag="ACHK74_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFP0221536.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPKGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            28091..29422
                     /gene="pepQ"
                     /locus_tag="ACHK74_002748"
     CDS             28091..29422
                     /gene="pepQ"
                     /locus_tag="ACHK74_002748"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFP0221537.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29422..30036
                     /gene="yigZ"
                     /locus_tag="ACHK74_002749"
     CDS             29422..30036
                     /gene="yigZ"
                     /locus_tag="ACHK74_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFP0221538.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            30075..31526
                     /gene="trkH"
                     /locus_tag="ACHK74_002750"
     CDS             30075..31526
                     /gene="trkH"
                     /locus_tag="ACHK74_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFP0221539.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31538..32083
                     /gene="hemG"
                     /locus_tag="ACHK74_002751"
     CDS             31538..32083
                     /gene="hemG"
                     /locus_tag="ACHK74_002751"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFP0221540.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7511 a         8156 c         8804 g         7771 t
ORIGIN      
        1 gaatatatat tacctgaatt atgtggtttg catatatatg caatatagca gtctttgcat
       61 tgataactat acagtagtaa tttagagtgt gtaagttttt attgatgagg tatattttaa
      121 ttaaatataa ttgaaatgta tttttattct ataaacaata tcttgggtga atggattcta
      181 tatgggaaat ggtaactttt ggcagttgtt cttgggtaaa gaaccttaca gcaaaaaatc
      241 ttattttgtt aaggcatggt tattgtggtg ggctattacc atccttgttc tatttatttt
      301 aagcctgact acgacattaa atttaagaag tataggtata gggttaagtt ttggcttttg
      361 tcaaatcctt ggtagatata cgatgtctct aggatggagt aagtactggg gagttttggg
      421 ggtatttcct gtaacaaata ttgttctttt tcttatctta atttttttca aaaaaagata
      481 aataagtcag tgggtaggct ctttaaacgg cctacccatg ttaattctta actttttcta
      541 cgctgcgtat aaatcgcatc catcacaaaa attgccagcg ccacccaaat aaaggcgaaa
      601 gtcaccatct tatcggcacc cggtttttca ccataaaacg tcacagccag caggaacatc
      661 agcgtcgggc caatgtactg gaaaaagcct aacgttgaga gacgcaagcg cgtggcagcg
      721 gcggtaaaac acaacagcgg tacggtagtg acaataccgg cggcgatcag cagtaaattc
      781 agcgacatcg ggttttgccc catatggctg gttgagctgt cggcaatagc aaacaggtaa
      841 attgccgcca cgggcagcag ccacatggtt tcgattaaca tgccggtttg cgcttcaacg
      901 gcaatcttct tgcgtaccag accgtagaag gcaaaactaa atgccagccc cagcgcgata
      961 ataggtagcg aaccaaaagt ccacagctgg accaacacgc cacatatcgc cagaatcacc
     1021 gccagccatt gcatccggcg gaatcgctcg ccgaggaaaa tcatccccag cacaatgttc
     1081 accagcgggt taataaagta accaaggctc gcttccagca tatggtgatt gttcaccgcc
     1141 cagataaaca gtagccagtt gccaccaatc agcacggcag agactgccag cataaaaatt
     1201 ttctgtggcg tctgaatcag cgtttttaaa taggaccact ggcggcaaat gctcatcagc
     1261 accaccataa agaaaaacga ccagatcacg cgatgcgtca ggatttcatc ggcgggcacg
     1321 tagtaaatca acttgaagta cgctggcgct ataccccaaa taaaataagc ggcaagagcg
     1381 agtaatacgc cctgccgcgt ttgttttgca tccatcggga atactcattt ttaattggta
     1441 acagcagttt acctgctttt atgtcttcaa cctaccatat aggtggcggt ggcactggca
     1501 atataaagct gttcttcatt gtgtaattca acgcgggcga cggcgacttt attgcctgca
     1561 cgcaacaggc tactggtagc agtaaaacgc tcgcccctgc ctgggcgcag ataatcaacg
     1621 cgaagatcaa tggtccccat ccgcgatagc cgctggcgta gttcatcttc actgatggtt
     1681 tcgtggcggg ttaaggtact tcccacgcac accagaccgg cggcgacatc cagcgccgac
     1741 gcaatgaccc cgccgtgcaa aatgctttgc gcccagttgc ccaccatcat tggctgattt
     1801 ttaaaggcca gctgtgcgaa ctctttttcg taacgctcca gttccatccc caatgcgcgg
     1861 ttaaatggca tgtgataaac aaacatctca cccactaatt tcagggcttg ttcagcagtc
     1921 agtacggcag acatatcatc cttacacttc attggttaat gaaatgttga ttttatgctt
     1981 ctttgttgtt ggtttctact ttaggaaggg ataactaacg gcaatggagt taagtatgta
     2041 aaatagcccg cagaaaaaca ttcaccttat caataattcg ttacggagaa cacgaccgat
     2101 gcggactctg cagggctggt tgttgccggt gtttatgttg cctatggcag tatatgcaca
     2161 agaggcaacg gtgaaagagg tgcatgacgc gccagcggtg cgtggcagta ttatcgccaa
     2221 tatgctgcag gagcatgaca atccgttcac gctctatcct tatgacacca actacctcat
     2281 ctacacacaa accagcgatc tgaataaaga agcgattgcc agttacgact gggcggaaaa
     2341 tgcgcgtaag gatgaagtaa agtttcagtt gagcctggca tttccactgt ggcgtgggat
     2401 tttaggcccg aactcggtgt tgggtgcatc ttatacgcaa aaatcctggt ggcaactgtc
     2461 caatagcgaa gagtcttcac cgtttcgtga aaccaactac gaaccgcaat tgttcctcgg
     2521 ttttgccacc gattaccgtt ttgcaggttg gacgctgcgc gatgtggaga tggggtataa
     2581 ccacgactct aacgggcgtt cagacccgac ctcccgcagc tggaaccgcc tttatactcg
     2641 cctgatggca gaaaacggta actggctggt agaagtgaag ccgtggtatg tggtgggtaa
     2701 tactgacgat aacccggata tcaccaaata tatgggttac taccagctta aaatcggcta
     2761 tcacctcggt gatgcggtgc tcagtgcgaa aggacagtac aactggaaca ccggctacgg
     2821 cggcgcggag ttaggcttaa gttacccgat caccaaacat gtgcgccttt atactcaggt
     2881 ttacagcggc tatggcgaat cgctcatcga ctataacttc aaccagaccc gtgtcggtgt
     2941 gggagttatg ctaaacgatt tgttttgatg aacggttgag ttgttggcaa atctggaatc
     3001 cagcatccag gattaccctc tcagagacta aaagcattgc agtttctcgc gcaggcgctg
     3061 aaaatagcgc ctgtttttat ttcaggcaat cggggtgaat gtggcgcagg cggaagtgtt
     3121 gaatctggag tccggagcta aacaggtttt acaagaaacc tttggctacc aacagtttcg
     3181 ccccggccag gaagaaatta tcgacactgt gctttccggt cgcgattgcc tggtcgtcat
     3241 gcccaccggt ggcggaaaat ccctttgcta tcaaattcct gccttattgc taaacggcct
     3301 taccgtggtt gtttcaccgc tgatttcgtt gatgaaagat caggtggatc aactgcaagc
     3361 caacggcgtg gcggcggcgt gccttaactc gacgcaaacc cgcgaacagc aacttgaagt
     3421 gatgacaggc tgccgcaccg ggcaaattcg tctgctttat atcgccccgg aacgcctgat
     3481 gctggataac tttcttgagc atctggcgca ctggaatccg gtgttattag ccgttgatga
     3541 agcgcactgt atctcccaat ggggccacga tttccgcccg gaatatgccg cgctcggtca
     3601 gttgcgccag cggttcccga cgctgccgtt tatggcgctg accgccacag ccgacgacac
     3661 cacgcgccag gatatcgtgc gcctgctggg gctgaacgat ccgctgattc aaatcaacag
     3721 ttttgaccgt ccgaatattc gctacatgct gatggagaag ttcaaaccgc tcgatcagtt
     3781 gatgcgctac gtgcaggaac agcgcggtaa gtccggcatt atctactgca acagccgggc
     3841 gaaagtagaa gacaccgctg cgcgcctgca aagcaagggt attagcgcgg cggcctatca
     3901 tgccgggctg gaaaataatg ttcgcgccga tgtgcaggaa aaattccagc gcgatgacct
     3961 gcaaattgtg gtggcgacgg tggcgttcgg catgggcatc aataaaccaa acgttcgctt
     4021 cgtggtccac tttgatattc cgcgcaatat cgaatcctat tatcaggaaa ccggtcgcgc
     4081 cgggcgtgat ggcctgcctg cggaagcgat gctgttttac gatccggctg atatggcgtg
     4141 gctgcgccgt tgtctggaag agaagccgca ggggcagttg caggatatcg agcgccacaa
     4201 actcaatgcg atgggcgcgt ttgccgaagc gcaaacttgc cgtcgtctgg tattgctgaa
     4261 ctattttggc gaagggcgtc aggagccgtg cgggaactgc gatatctgcc tcgatccgcc
     4321 gaaacagtac gacggttcaa ccgatgctca gattgccctt tccaccattg gtcgtgtgaa
     4381 tcagcggttt gggatgggtt atgtggtgga agtgattcgt ggtgctaata accagcgtat
     4441 ccgcgactat ggtcatgaca aactgaaagt ctatggcatg ggccgtgata aaagccatga
     4501 acattgggtg agcgtgatcc gccagctgat tcacctcggc ctggtgacgc aaaatattgc
     4561 ccagcattct gccctccaac tgacagaggc cgcgcgtccg gtgctgcgcg gcgaatcctc
     4621 tttgcaactt gccgtgccgc gtatcgtggc gctcaaaccg aaagcgatgc agaaatcgtt
     4681 cggcggcaac tatgatcgca aactgttcgc caaattacgc aaactgcgta aatcgattgc
     4741 cgatgaaagt aatgtcccgc cgtacgtggt gtttaacgac gcaaccttga ttgagatggc
     4801 tgaacagatg ccgatcaccg ccagcgaaat gctcagcgtt aacggcgttg ggatgcgcaa
     4861 gctggaacgc tttggcaaac cgtttatggc gctgattcgt gcgcatgttg atggcgatga
     4921 cgaagagtag tcagcagcat aaaaaagtgc caggatgaag actccgtaaa cgtttccccc
     4981 gcgagtcaaa tgtatgttga tgttatttct caccgtcgcc atggtgcaca ttgtggcgct
     5041 tatgagcccc ggtcccgatt tcttttttgt ctctcagacc gctgtcagtc gttcccgtaa
     5101 agaagcgatg atgggcgtgc tgggcattac ctgcggcgta atggtttggg ctgggattgc
     5161 gttgcttggc ctgcatttga ttatcgaaaa aatggcctgg ctgcatacgc tgattatggt
     5221 tggcggtggc ctgtatctct gctggatggg ttaccagatg ctgcgcggcg cgctgaaaaa
     5281 agaggcagtt tctgcacctg cgccacaggt cgagctagcg aaaagtgggc gcagtttcct
     5341 gaaaggttta ctgaccaatc tcgctaatcc gaaagcgatt atctactttg gctcggtgtt
     5401 ctcattgttt gtcggtgata acgttggcac taccgcgcgc tggggcattt ttgcgctgat
     5461 cattgtcgaa acgctggcgt ggtttaccgt cgttgccagc ctgtttgccc tgccgcaaat
     5521 gcgccgtggt tatcaacgtc tggcgaagtg gattgatggt tttgccgggg cgttatttgc
     5581 cggatttggc attcatttga ttatttcgcg gtgatgccag acgcgtcttc agagtaagtc
     5641 ggataaggcg tttacgccgc atccgacatt atttttcacg catgcctcgc cgatgctaac
     5701 agcgctccca ccagcataaa caacgagccg aaaatcttat tcagcgcctt catctgcttt
     5761 ggtcctttaa tccatagagc aatccgttga gcaagggtgg cgtaaccgat catcacaata
     5821 atatcgacca caatagtggt gacgccgagc acgatatact gcatcagttg cggctgttgc
     5881 ggcatgatga attgcggaaa tagcgccgcc agaaacacaa tacttttggg attggtgaga
     5941 ttcacaaaaa ctgcgcgctg gaacaaatgt cgacgcgatt gagtagaggc cagcgattta
     6001 aggtcaattg caccagcggc gcgccactgc tggattccca gccaaatcaa gtaagccgcg
     6061 cctgcccact tcaacacttc aaacgcaatc actgagcggg aaaatagcgt ccccaacccc
     6121 acgccaacca gcacaatatg aatcgccagt ccggtctgaa gcccagcaat agacgccacc
     6181 gcgccgcgat aaccgtggtt gagcgaggtg gtcatagtgt tgattgcacc agagcctggc
     6241 gacaggctta aaatgatcga tgtcagcagg taggcaaacc accattctaa ggtcatgatg
     6301 aactcccggt gtgtctattt ttgtgccaca atacgctact gtcgcagcgt tgtgtcaggc
     6361 acgctaaaaa aaaacgattt tacgtggttt aagaggcaga ttacccgatg tttcagcagc
     6421 aaaaagactg ggaaacaaga gaaaacgcgt ttgctgcttt taccatggga ccgctgactg
     6481 atttctggcg tcagcgtgat gaagcagagt ttactggtgt ggatgacatt ccggtgcgct
     6541 ttgtccgttt tcgcgcacag caccatgacc gggtggtagt catctgcccg gggcgtattg
     6601 agagctacgt aaaatatgcg gaactggcct atgacctgtt ccatttgggg tttgatgtct
     6661 taatcatcga ccatcgcggg cagggacgtt ccggtcgcct gttagccgat ccgcatctcg
     6721 ggcatgttaa tcgctttaat gattatgttg atgatctggc ggcattctgg caacaggagg
     6781 tccagcccgg tccgtggcgt aaacgctata tactggcaca ttcgatgggc ggtgcgatct
     6841 ccacattatt tctgcaacgc catccaggtg tatgtgacgc cattgcgcta actgcgccaa
     6901 tgtttgggat cgtgattcgt atgccgtcat ttatggcacg gcagatcctc aactgggccg
     6961 aagcgcatcc acgtttccgt gatggctatg caataggcac cgggcgctgg cgcgcgttgc
     7021 cgtttgctat caacgtactg acccacagca gacagcgata tcgacgtaac ttacgcttct
     7081 atgctgatga cccaacgatt cgcgtcggtg ggccgaccta ccattgggta cgcgaaagta
     7141 ttctggctgg cgaacaggtg ttagccggtg cgggtgatga cgccacgcca acgcttctct
     7201 tgcaggctga agaggaacgc gtggtggata accgcatgca tgaccgtttt tgtgaactcc
     7261 gcaccgccgc gggccatcct gtcgaaggag gacggccgtt ggtaattaaa ggtgcttacc
     7321 atgagatcct ttttgaaaag gacgcaatgc gctcagtcgc gctccacgcc atcgttgatt
     7381 ttttcaacag gcataactca cccagcggaa accgctctac agaggtttaa atttcttatg
     7441 taccaggttg ttgcgtctga tttagatggc acgttacttt ctcccgacca tacgttatcc
     7501 ccttacgcca aagaaactct gaagctgctc accgcgcgcg gcatcaactt tgtgtttgcg
     7561 accggtcgtc accacgttga tgtggggcaa attcgcgata atctggagat taagtcttac
     7621 atgattacct ccaatggtgc gcgcgttcac gatctggatg gtaatctgat ttttgctcat
     7681 aacctggatc gcgacattgc cagcgatctg tttggcgtag tcaacgacaa tccggacatc
     7741 attactaacg tttatcgcga cgacgaatgg tttatgaatc gccatcgccc ggaagagatg
     7801 cgctttttta aagaagcggt gttcaaatat gcgctgtatg agcctggatt actggagccg
     7861 gaaggcgtca gcaaagtgtt cttcacctgc gattcccatg aacaactgct gccgctggag
     7921 caggcgatta acgctcgttg gggagatcgc gtcaacgtca gtttctctac cttaacctgt
     7981 ctggaagtga tggcgggcgg cgtttcaaaa ggccatgcgc tggaagcggt ggcgaagaaa
     8041 ctgggctaca gcctgaagga ttgtattgcg tttggtgacg ggatgaacga cgccgaaatg
     8101 ctgtcgatgg cggggaaagg ctgcattatg ggcagtgcgc accagcgtct gaaagacctt
     8161 catcccgagc tggaagtgat tggtactaat gccgacgacg cggtgccgca ttatctgcgt
     8221 aaactctatt tatcgtaatc gttctttatt tggtcagttg tcaacctgat acttcgctac
     8281 aatggatact cgttaatcaa agagttttcc attgtggcgc tacttatcat caccacgatt
     8341 ctgtgggcct tctcctttag cttttatggc gagtaccttg cggggcacgt cgatagctat
     8401 tttgcggtgc tggtgcgcgt tggcctggcg gcactcgttt ttctgccgtt tctgcgtacc
     8461 cgtggcaata gcctgaaaac agtcggcctg tatatgctgg tgggcgcgat gcagcttggc
     8521 gtgatgtata tgctgagttt ccgcgcttat ctctatctga cggtttccga actgctgctg
     8581 ttcaccgtac tgacgccgct ctacatcacg ctgatttatg acatcatgag taagcgccgt
     8641 ctgcgctggg gatatgcttt tagtgccttg ttggcggtga ttggtgccgg gattattcgc
     8701 tatgatcagg tcaccgacca tttctggact ggcttgttgc tggtgcaact ctccaatatc
     8761 acttttgcca tcggcatggt gggttacaaa cgcctgatgg aaactcgccc gatgccgcag
     8821 cataacgcct ttgcgtggtt ctatcttggc gcgttcctgg tggcggtaat tgcatggttc
     8881 ttgctgggaa atgcgcagaa aatgccgcaa accacgttgc aatggggcat tctggtgttt
     8941 cttggcgtgg tggcttccgg gattggctac tttatgtgga actacggcgc gacgcaggtg
     9001 gacgccggaa cgctgggaat tatgaataat atgcacgttc cggcagggct gctggtaaac
     9061 ctggctatct ggcaccaaca gccgcactgg ccaacgttta ttacaggcgc gctggtgatc
     9121 ctggcctcac tgtgggtgca tcgtaagtgg gtcgctccgc gctcttcaca aacggcagat
     9181 gatcgcaggc gtgattgcgc gctgagcgaa taaacgcttc cgtaactggc tgacgctgct
     9241 cgccatcgcg cacggcggcg tacagtcggc tccacaagcc ttcgcccagg gttttggtca
     9301 ccaccagacc ctggcgctca aaactctcta ctacccaatg cggtagcgcg gcaataccca
     9361 tccgcgcggc aaccatctga atcaacaata aggtgttatc gacgcttttt agtgacggac
     9421 tgacgcctgc cggctgaaga aaatgccgcc agacatccag tcgactacgc tgcaccggat
     9481 aaattaatag cgtctcgctg gcgagatctt ccggtgtaat acgcgttttt gccgccagtg
     9541 gatggtcagg tgctaacacc agacgcactt catagtcgaa catcggcgaa taatgcagac
     9601 cactgcgcgg cagaatatcg gacgtcatta ccagatccag ctcgccctgt tgcaacgcgg
     9661 gctgcgggtc aaacgtcacg cccgatttaa aatccatctc tacctgcggc cagttcttat
     9721 ggaaattttc taacgcgggc gtcagccact gaatacagct atggcactca atggcaatgc
     9781 gcagacgcgt ctgctgcggt tcattgcagg cttgcagtgc ctggctaatt tgcggcagca
     9841 cctggtttgc cagttgcaac aggatttctc cctgcggtgt aaagcgtagc ggctggctct
     9901 tacgcacaaa tagccggaag ccaaggcgtt gttccagatc gctaaactgg tgagacaggg
     9961 cggattgcgt ctgatgcaac gtcgccgcag cggctgcgag agagccgcag ttccgcaacg
    10021 cttgtagcgt tttcaggtgt tttacttcga tcatgaaagt ccttcacttc ggcatgaata
    10081 atttgcgctt gaggaatata cagtaaccgc caattatgga tgtgtaaaca tctggacggc
    10141 taaatactaa aaattccata aggggcatat aatgacaatt cttaatcaca ccctcggttt
    10201 ccctcgcgtt ggcctgcgtc gcgagctgaa aaaagcgcaa gaaagttatt gggcggggaa
    10261 ctccacgcgt gaagaactgc tgacagtagg gcgtgaactg cgtgctcgtc actgggatca
    10321 acaaaagcaa gcgggtatcg acctgctgcc ggtgggcgat tttgcctggt acgatcatgt
    10381 tctgaccacc agtctgctgc tgggtaatgt tccgccacgt catcagaaca aagacggttc
    10441 ggtagatatc gacaccctgt tccgtattgg tcgtggacgt gcgccgactg gcgaacctgc
    10501 ggcggcagcg gaaatgacca aatggtttaa caccaactat cactacatgg tgccggaatt
    10561 cgttaaaggc caacagttca aactgacctg gacgcagctg ctggaggaag tggacgaggc
    10621 gctggcgctg ggccacaacg ttaagcctgt gctgctgggg ccggttactt acctgtggct
    10681 ggggaaagtg aaaggtgaac agtttgaccg ccttagcctg ctgaacgaca ttctgccggt
    10741 ttatcagcaa gtgctggcag aactggcgaa acgcggcatc gagtgggtac agattgatga
    10801 acccgcgctg gtactggaac taccacaggc gtggctggac gcatacaaac ccgcttacga
    10861 cgcgctccag ggacaggtga aactgctgct gaccacctat tttgaaggcg taacgccaaa
    10921 tctcgacacg attactgcgc tgcctgttca gggtctgcat gttgacctcg tacatggtaa
    10981 agatgacgtt gctgaactgc acaagcgcct gccttctgac tggttgctgt ctgcgggtct
    11041 gatcaatggt cgtaacgtct ggcgcgccga tcttaccgag aaatatgcgc aaattaagga
    11101 cattgtcggc aaacgtgatt tgtgggtggc atcttcctgc tcgttgctgc acagccccat
    11161 cgacctgagc gtggaaacgc gtcttgatgc agaagtgaaa agctggtttg ccttcgccct
    11221 acaaaaatgc catgaactgg cactgctgcg cgatgcgctg aacagtggtg acacggcagc
    11281 tctggcagag tggagcgccc cgattcaggc acgtcgtcac tctacccgcg tacataatcc
    11341 ggcggtagaa aagcgtctgg cggcgatcac cgcccaggac agccagcgtg cgaatgtcta
    11401 tgaagtgcgt gccgaagccc agcgtgcgcg ttttaaactg ccagcgtggc cgaccaccac
    11461 gattggttct ttcccgcaaa ccacggaaat tcgtaccctg cgtctggatt tcaaaaaggg
    11521 caatctcgac gccaataact accgcacggg cattgcggaa catatcaggc aggccattgt
    11581 tgaacaggaa cgtttgggac tggatgtgct ggtacatggc gaggccgagc gtaatgacat
    11641 ggtggaatac tttggcgagc acctcgacgg gtttgtcttt acgcaaaacg gttgggtaca
    11701 gagctacggt tcccgctgcg tgaagccacc gattgttatt ggtgacgtta gtcgcccggc
    11761 accgattacc gtggagtggg cgaagtatgc gcaatcgctg accgacaaac cggtgaaagg
    11821 gatgctgacg gggccggtga ccatactctg ctggtcgttc ccgcgtgaag atgtcagccg
    11881 tgaaaccatc gccaaacaga ttgcgctggc gctgcgtgat gaggtggccg atctggaagc
    11941 ggcagggatt ggtatcatcc agattgacga accggcgctg cgcgaaggtt taccactgcg
    12001 tcgtagcgac tgggacgcct atctccagtg gggcgtagag gccttccgta tcaacgccgc
    12061 cgtggcgaaa gatgacacac aaatccacac tcacatgtgt tattgcgagt tcaacgacat
    12121 catggattcc attgcggcgc tggatgcaga cgtcatcacc atcgaaacct cgcgttccga
    12181 catggagttg ctggagtcgt tcgaagagtt tgattatccg aatgaaatcg gtccaggcgt
    12241 ctatgacatt cactcgccaa acgtaccgag cgtggaatgg attgaagccc tgctgaagaa
    12301 agcggcaaaa cgcattccgg cagagcgcct gtgggtcaac ccggactgtg gcctgaaaac
    12361 gcgcggctgg ccagaaaccc gcgcggcact ggcgaacatg gtgcaggcgg cgcagaattt
    12421 gcgtcggggg taaaatccaa accgggtggt aataccaccc ggtcttttct cattacagcg
    12481 acttcttccc accatactgc ttaaaccatt ccagcatacg ctgccagcca tctttcgcag
    12541 attcggcatg atagctcggg cgataatcag cgttgaatgc atgcccggca tccgggtaga
    12601 cgataatctc tgctttcgca ttagcagccc gcagcgcctg gcgcatggtt tcaacgctct
    12661 cctgcggaat actgttatcc agaccgccat ataagccgag aaccggcgcg ttaagatcgg
    12721 ttgcgatatc aacaggttgt ttcggtgaat tcagcgactt atcgcccgtc agtttgccgt
    12781 accacgccac tgcggctttt agctgtggat tatgcgcggc atacagccag gtgatacgtc
    12841 caccccagca gaatccggtg atcattaaac gatgaacatc gccgccgttg cgggaagccc
    12901 aactggcgac atgatcgaga tcggccagca cctgcgagtc aggcactttt gctaccagac
    12961 cgctaagcaa cgtggggata tcggcaaaat cattcggatc gccttcgcgg aagtaaagtt
    13021 caggtgcgat agccagatac ccctccagcg ccagacggcg acaaatgtcg cggatatgtt
    13081 catgcacgcc aaaaatttcc tgcactacaa tgaccactgg cagttggcca tcgctttgct
    13141 ttggtctggc atggtaagca ggcatgttat ccccttgtga agggatagag gtgaagcccg
    13201 ccacaatcgc gtcgtccggg gtctgaacga tggtcgaagc gagaggcgat gcagcaggtg
    13261 taaatccaga ttgttgtgtt gttgccatgg tattctccgt acccttataa aaatgttgcg
    13321 caatgttaac tatagtcagc atgcaacaaa tcacattgcc tgaatcggct catcttttat
    13381 gcagtcctgc agaatgaaga gtgatttatg tgatttgaat cacttttggt gggtaaattt
    13441 atgcaacgca tttgcgccat ggtgatgagt atcacgaaaa aatgttaaac ccttcggtaa
    13501 agtgtctttt tgcttcttct gactaaaccg attcacagag gagttgtata tgtccaagtc
    13561 tgatgttttt catctcggcc tcactaaaaa cgatttacaa ggggctacgc ttgccatcgt
    13621 ccctggcgac ccggatcgtg tggaaaagat cgccgcgctg atggataagc cggttaagct
    13681 ggcatctcac cgcgaattca ctacctggcg tgcagagctg gatggtaaac ctgttatcgt
    13741 ctgctctacc ggtatcggcg gcccgtctac ctctattgct gttgaagagc tggcacagct
    13801 gggcattcgc accttcctgc gtatcggtac aacgggcgct attcagccgc atattaatgt
    13861 gggtgatgtc ctggttacca cggcgtctgt ccgtctggat ggcgcgagcc tgcacttcgc
    13921 accgctggaa ttcccggctg tcgctgattt cgaatgtacg actgcgctgg ttgaagctgc
    13981 gaaatccatt ggcgcgacaa ctcacgttgg cgtgacagct tcttctgata ccttctaccc
    14041 aggccaggaa cgttacgata cttactctgg tcgcgtagtt cgtcacttta aaggttctat
    14101 ggaagagtgg caggcgatgg gcgtaatgaa ctatgaaatg gaatctgcaa ccctgctgac
    14161 catgtgtgca agtcagggcc tgcgtgccgg tatggtagcg ggtgttatcg ttaaccgcac
    14221 ccagcaagag atcccgaatg ctgagacgat gaaacaaact gaaagccaag cggtgaaaat
    14281 cgtggtggaa gcggcgcgtc gtctgctgta attctcttct cctgtctgaa ggccgacgcg
    14341 ttcggccttt tgtatttttg cgtagcgcct cgcaggaaat gcctttccaa ctggacgttt
    14401 gtacagcaca attctatttt gtgcgggtaa gttgttgcgt caggaggcgt tgtggatttc
    14461 tcaatcatgg tttacgcagt tattgcgttg gtgggtgtgg caattggctg gctgtttgcc
    14521 agttatcaat atgcgcagca aaaagccgag caattagctg aacgtgaaga gatggtcgcg
    14581 gagttaagcg cggcaaaaca acaaattacc caaagcgagc actggcgtgc agagtgcgag
    14641 ttactcaata acgaagtgcg cagcctgcaa agtattaaca cctctctgga ggccgatctg
    14701 cgtgaagtaa ccacgcggat ggaagccgca cagcaacatg ctgacgataa aattcgccag
    14761 atgattaaca gcgagcagcg cctcagtgag cagtttgaaa acctcgccaa ccgtattttt
    14821 gagcacagca atcgccgggt tgatgagcaa aaccgtcaga gtctgaacag cctgttgtcg
    14881 ccgctacgtg aacaactgga cggtttccgc cgtcaggttc aggacagctt cggtaaagaa
    14941 gcacaagaac gccataccct gacccacgaa attcgcaatc tccagcaact caacgcgcaa
    15001 atggcccagg aagcgatcaa cctgacgcgc gcgctgaaag gcgacaataa aacccagggc
    15061 aactggggcg aggtagtatt gacgcgggtg ctggaggctt ccggtctgcg tgaagggtat
    15121 gaatatgaaa cccaggtcag catcgaaaat gacgcccgct cgcggatgca gccggatgtc
    15181 atcgtgcggc tgccgcaggg aaaagatgtg gtgatcgacg ccaaaatgac gctggtcgcc
    15241 tatgaacgct attttaacgc cgaagacgac tacacccgcg aaagcgcgct acaggaacat
    15301 atcgcgtcgg tgcgtaacca tatccgtttg ctgggacgca aagattatca acagctgccg
    15361 gggctgcgaa ctctggatta cgtgctgatg tttattcccg ttgaacccgc tttttcactg
    15421 gcgcttgacc gccagccgga gctgatcacc gaagcgttga aaaacaatat catgctggtt
    15481 agcccgacta cgctgctggt ggcgttgcgt actattgcca acctgtggcg ttatgagcat
    15541 caaagccgca acgcccagca aatcgccgat cgtgccagca agctgtacga caagatgcgt
    15601 ttgttcatcg atgacatgtc cgcgattggt caaagtctcg acaaagcgca ggataactac
    15661 cgccaggcaa tgaaaaaact ctcttcaggt cgcggaaatg tgctggcgca ggcagaagcg
    15721 tttcgcggtt taggagtaga aattaaacgc gagattaatc cggatttggc tgaacaggcg
    15781 gtgagccagg atgaagagta tcgacttcgg tcggttccgg agcagccgaa tgatgaagct
    15841 tatcaacgcg atgatgaata taatcagcag tcgcgctagc ccattagggg tagttaagcc
    15901 gggtagaaat ctagggcatc gacgcccaat ctgttacact tctggaacaa ttttttgatg
    15961 agcaggcatt gagatggtgg ataagtcaca agaaacgacg cactttggtt ttcagaccgt
    16021 cgcgaaggaa caaaaagcgg atatggtcgc ccacgttttc cattccgtgg catcaaaata
    16081 cgatgtcatg aatgatttga tgtcatttgg tattcatcgt ttgtggaagc gattcacgat
    16141 tgattgcagc ggcgtacgcc gtgggcagac cgtgctggat ctggctggtg gcaccggcga
    16201 cctgacagcg aaattctccc gcctggtcgg agaaactggc aaagtggtcc ttgctgatat
    16261 caatgaatcc atgctcaaaa tgggacgcga gaagctgcgt aatatcggtg tgattggcaa
    16321 cgttgagtat gttcaggcga acgctgaggc gctgccgttc ccggataaca cctttgattg
    16381 catcaccatt tcgtttggtc tgcgtaacgt caccgacaaa gataaagcac tgcgttcaat
    16441 gtatcgcgtg ctgaaacccg gcggccgcct gctggtgctt gagttctcga agccaattat
    16501 cgagccgctg agcaaagcct atgatgcata ctccttccat gtgctgccgc gtattggctc
    16561 actggtcgcg aacgacgccg acagctaccg ttatctggca gaatccatcc gtatgcatcc
    16621 cgatcaggat accctgaaag ccatgatgca ggatgccgga ttcgaaagtg tcgactacta
    16681 caatctgacg gcgggggttg tggcgctgca tcgtggttat aagttctgac aggagaccgg
    16741 aaatgccttt taaaccttta gtgacggcag gaattgaaag tctgctcaac accttcctgt
    16801 atcgctcacc cgcgctgaaa acagcccgct cgcgtctgct gggtaaagta ttgcgcgtgg
    16861 aggtaaaagg cttttcgacg tcattgattc tggtgttcag cgaacgccag gttgatgtac
    16921 tgggcgaatg ggcaggcgat gctgactgca ccgttatcgc ctacgccagt gtgttgccga
    16981 aacttcgcga tcgccagcag cttaccgcac tgattcgcag tggtgagctg gaagtgcagg
    17041 gcgatattca ggtggtgcaa aacttcgttg cgctggcaga tctggcagag ttcgaccctg
    17101 cggaactgct ggccccttat accggtgata tcgccgctga aggaatcagc aaagccatgc
    17161 gcggaggcgc aaagttcctg catcacggca ttaagcgcca gcaacgttat gtggcggaag
    17221 ccattactga agagtggcgt atggcacccg gtccgcttga agtggcctgg tttgcggaag
    17281 agacggctgc cgtcgagcgt gctgttgatg ccctgaccaa acggctggaa aaactggagg
    17341 ctaaatgacg ccaggtgaag tacggcgcct atatttcatc attcgcactt ttttaagcta
    17401 cggacttgat gaactgatcc ccaaaatgcg tatcaccctg ccgctacggc tatggcgata
    17461 ctcattattc tggatgccaa atcggcataa agacaaactt ttaggtgagc gactacgact
    17521 ggccctgcaa gaactggggc cggtttggat caagttcggg caaatgttat caacccgccg
    17581 cgatcttttt ccaccgcata ttgccgatca gctggcgtta ttgcaggaca aagttgctcc
    17641 gtttgatggc aagctggcga agcagcagat tgaagctgca atgggcggct tgccggtaga
    17701 agcgtggttt gacgattttg aaatcaagcc gctggcttct gcttctatcg cccaggttca
    17761 taccgcgcga ttgaaatcga atggtaaaga ggtggtgatt aaagtcatcc gcccggatat
    17821 tttgccggtc attaaagcgg atctgaaact tatctaccgt ctggctcgct gggtgccgcg
    17881 tttgctgccg gatggtcgcc gtctgcgccc aaccgaagtg gtgcgcgagt acgaaaagac
    17941 cttgattgat gaactgaatt tgctgcggga atctgccaac gccattcagc ttcggcgcaa
    18001 ttttgaagac agcccgatgc tctacatccc ggaagtttac cctgactatt gtagtgaagg
    18061 gatgatggtg atggagcgca tttacggcat tccggtgtct gatgttgcgg cgctggagaa
    18121 aaacggcact aacatgaaat tgctggcgga acgcggcgtg caggtgttct tcactcaggt
    18181 ctttcgcgac agctttttcc atgccgatat gcaccctggc aacatcttcg taagctatga
    18241 acacccggaa aacccgaaat atatcggcat tgattgcggg attgttggct cgctaaacaa
    18301 agaagataaa cgctatctgg cagaaaactt tatcgccttc tttaatcgcg actatcgcaa
    18361 agtggcagag ctacacgtcg attcaggctg ggtgccacca gataccaacg ttgaagagtt
    18421 cgaatttgcc attcgtacgg tctgtgaacc tatctttgag aaaccgctgg ccgaaatttc
    18481 gtttggacat gtactgttaa atctgtttaa tacggcgcgt cgcttcaata tggaagtgca
    18541 gccgcaactg gtgttactcc agaaaaccct gctctatgtc gaaggggtag gacgccagct
    18601 ttatccgcag ctcgatttat ggaaaacagc gaagcctttc ctggagtcgt ggattaaaga
    18661 tcaggtcggt attcctgcgc tggtgagagc atttaaagaa aaagcgccgt tctgggtcga
    18721 aaaaatgcca gaactgcctg aattggttta cgacagtttg cgccagggca agtatttaca
    18781 gcacagtgtt gataagattg cccgcgagct tcagtcaaat catgtacgtc agggacaatc
    18841 gcgttatttt ctcggaattg gcgctacgtt agtattaagt ggcacattct tgttggtcag
    18901 ccgacctgag tgggggctga tgcccggctg gttaatggca ggtggtctga tcgcctggtt
    18961 tgtcggttgg cgcaaaacac gctgattttt tcatcgctca aggcgggccg tgtaacgtat
    19021 aatgcggctt tgtttaatca tcatctacca cagaggaaca tgtatgggtg gtatcagtat
    19081 ttggcagtta ttgattattg ccgtcatcgt tgtactgctt tttggcacca aaaagctcgg
    19141 ctccatcggt tccgatcttg gtgcgtcgat caaaggcttt aaaaaagcaa tgagcgatga
    19201 tgaaccaaag caggataaaa ccagtcagga tgctgatttt actgcgaaaa ctatcgccga
    19261 taagcaggcg gatacgaatc aggaacaggc taaaacagaa gacgcgaagc gccacgataa
    19321 agagcaggtg taatccgtgt ttgatatcgg ttttagcgaa ctgctattgg tgttcatcat
    19381 cggcctcgtc gttctggggc cgcaacgact gcctgtggcg gtaaaaacgg tagcgggctg
    19441 gattcgcgcg ttgcgttcac tggcgacaac ggtgcagaac gaactgaccc aggagttaaa
    19501 actccaggag tttcaggaca gtctgaaaaa ggttgaaaag gcgagcctca ctaacctgac
    19561 gcccgaactg aaagcgtcga tggatgaact acgccaggcc gcggagtcga tgaagcgttc
    19621 ctacgttgca aacgatcctg aaaaggcgag cgatgaagcg cacaccatcc ataacccggt
    19681 ggtgaaagat aatgaagctg cgcatgaggg cgtaacgcct gccgctgcac aaacgcaggc
    19741 cagttcgccg gaacagaagc cagaaaccac gccagagccg gtggtaaaac ctgctgcgga
    19801 cgctgaaccg aaaaccgctg caccttcccc ttcgtcgagt gataaaccgt aaacatgtct
    19861 gtagaagata ctcaaccgct tatcacgcat ctgattgagc tgcgtaagcg tctgctgaac
    19921 tgcattatcg cggtgatcgt gatattcctg tgtctggtct atttcgccaa tgacatctat
    19981 cacctggtat ccgcgccatt gatcaagcag ttgccgcaag gttcaacgat gatcgccacc
    20041 gacgtggcct cgccgttctt tacgccgatc aagctgacct ttatggtgtc gctgattctg
    20101 tcagcgccgg tgattctcta tcaggtgtgg gcatttatcg ccccagcgct gtataagcat
    20161 gaacgtcgcc tggtggtgcc gctgctggtt tccagctctc tgctgtttta tatcggcatg
    20221 gcattcgcct actttgtggt ctttccgctg gcatttggct tccttgccaa taccgcgccg
    20281 gaaggggtgc aggtatccac cgacatcgcc agctatttaa gcttcgttat ggcgctgttt
    20341 atggcgtttg gtgtctcctt tgaagtgccg gtagcaattg tgctgctgtg ctggatgggg
    20401 attacctcgc cagaagactt acgcaaaaaa cgcccgtatg tgctggttgg tgcattcgtt
    20461 gtcgggatgt tgctgacgcc gccggatgtc ttctcgcaaa cgctgttggc gatcccgatg
    20521 tactgtctgt ttgaaatcgg tgtcttcttc tcacgctttt acgttggtaa agggcgaaat
    20581 cgggaagagg aaaacgacgc tgaagcagaa agcgaaaaaa ctgaagaata aattcaaccg
    20641 cccgtcaggg cggttgtcat atggagtaca ggatgtttga tatcggcgtt aatttgacca
    20701 gttcgcaatt tgcgaaagac cgtgatgatg ttgtagcgcg cgcttttgac gcgggagtta
    20761 atgggctact catcaccggc actaacctgc gtgaaagcca gcaggcgcaa aagctggcgc
    20821 gtcagtattc gtcctgttgg tcaacggcgg gcgtacatcc tcacgacagc agccagtggc
    20881 aagctgcgac tgaagaagcg attattgagc tggccgcgca gccagaagtg gtggcgattg
    20941 gtgaatgtgg tctcgacttt aaccgcaact tttcgacgcc ggaagagcag gaacgcgctt
    21001 ttgttgctca gctacgcatt gccgcagaat taaacatgcc ggtatttatg cactgtcgcg
    21061 atgcccacga gcggtttatg acattgctgg agccgtggct ggataaactg cctggtgcgg
    21121 ttcttcattg ctttaccggc acacgcgaag agatgcaggc gtgcgtggcg cgtggaattt
    21181 atatcggcat taccggttgg gtttgcgatg aacgacgcgg gctggagctg cgggaattgt
    21241 tgccgttgat tccggcggag aaattgctga tcgaaactga tgcgccgtat ctgctcccgc
    21301 gcgatctcac gccaaagcca tcatcccggc gcaacgagcc agcccatctg ccccatattt
    21361 tgcaacgtat tgcgcactgg cgtggagaag atgccgcatg gctggctgcc accacggatg
    21421 ccaatgtcaa aacactgttt gggattgcgt tttagagttt gcggaactcg gtgttcttca
    21481 cactgtgctt aatctcttta ttaataagat taagcaatag catggagcga gcctcaccat
    21541 cgggttcggt gaaaatggcc tgaaagcctt cgaacgcgcc ttcggtaata atcaccttat
    21601 cacccggata aggggttgcc ggatcgacaa tgtctttcgg tttatatacc gatagctgat
    21661 gaatcaccgc cgatgggact atcgctggcg acgcgccaaa gcgcacgaag tggctgacac
    21721 cgcgggtcgc gttgatagtc gtggtatgaa tcacttctgg atcaaattcg acaaacaggt
    21781 agttgggaaa caatggctca ctgactgcag tacgttttcc acgcacgatt ttttccaggg
    21841 tgatcatcgg tgccaggcaa ttcacagcct gtctttcgag gtgttcctgg gcacgttgaa
    21901 gttgcccgcg cttgcagtac agtaaatacc aggattgcat aatgactctt atccgtttaa
    21961 tcggggcgca aggatagcaa aagctttacg ctaagttaat tatattcccc ggtttgcgtt
    22021 ataccgtcag agttcacgct aatttaacaa atttacagca tcgcaaagat gaacgccgta
    22081 taatgggcgt agattaagag gctacaatgg acgccatgaa atataacgat ttacgcgact
    22141 tcttgacgct gcttgaacag cagggtgagc taaaacgtat cacgctcccg gtggatccgc
    22201 atctggaaat cactgaaatt gctgaccgca ctttgcgtgc cggtgggcct gcgctgttgt
    22261 tcgaaaaccc taaaggctac tcaatgccgg tgctgtgcaa cctgttcggt acgccaaagc
    22321 gcgtggcgat gggcatgggg caggaagatg tttcggcgct gcgtgaagtt ggtaaattat
    22381 tggcgtttct gaaagagccg gagccgccaa aaggtttccg cgacctgttt gataaactgc
    22441 cgcagtttaa gcaagtattg aacatgccga caaagcggct gcgtggtgcg ccctgccaac
    22501 aaaaaatcgt ctctggcgat gacgtcgatc tcaatcgcat tcccattatg acctgctggc
    22561 cggaagatgc cgcgccgctg attacctggg ggctgacagt gacgcgcggc ccgcataaag
    22621 agcggcagaa tctgggcatt tatcgccagc agctgattgg taaaaacaaa ctgattatgc
    22681 gctggctgtc gcatcgcggc ggcgcgctgg attatcagga gtggtgtgcg gcgcatccgg
    22741 gcgaacgttt cccggtttct gtggcgctgg gtgccgatcc cgccacgatt ctcggtgcag
    22801 tcacccccgt tccggataca ctttcagagt atgcgtttgc cggattgctg cgcggtacca
    22861 agaccgaagt ggtgaagtgt atctccaatg accttgaagt gcccgccagt gcggagattg
    22921 tgctggaagg gtatatcgaa caaggtgaaa cggcgccgga agggccgtat ggcgaccaca
    22981 ccggttacta taacgaagtc gatagtttcc cggtatttac cgtgacgcat attacccagc
    23041 gtgaagatgc gatttaccat tccacctata ccgggcgtcc gccagatgag cccgcggtac
    23101 tgggcgtggc gttgaacgaa gtgtttgtac cgattctgca aaaacagttc ccggaaattg
    23161 tcgattttta cctgccgccg gaaggctgtt cttatcgtct ggcggtagtg acgatcaaaa
    23221 aacagtacgc cggacacgcg aagcgcgtca tgatgggcgt ctggtcgttc ttacgccagt
    23281 ttatgtacac taaatttgtg atcgtttgcg atgatgacgt caacgcacgc gactggaacg
    23341 atgtgatttg ggcgattacc acccgtatgg acccggcgcg ggatactgtt ctggtagaaa
    23401 atacgcctat tgattatctg gattttgcct cgcctgtctc cgggctgggt tcaaaaatgg
    23461 ggctggatgc cacgaataaa tggccggggg aaacccagcg tgaatgggga cgtcccatca
    23521 aaaaagatcc agatgttgtc gcgcatattg acgccatctg ggatgaactg gctattttta
    23581 acaacggtaa aagcgcctga tgcgcgtttg ttttgcccta tttatcgatc cgacagagaa
    23641 agcgcatgac aaccttaagc tgtaaagtga cctcggtaga agctatcacg gataccgtat
    23701 atcgtgtccg catcgtgcca gacgcggcct tttcttttcg tgctggtcag tatttgatgg
    23761 tagtgatgga tgagcgcgac aaacgtccgt tctcaatggc ctcgacgccg gatgaaaaag
    23821 ggtttatcga gctgcatatt ggcgcttctg aaatcaacct ttacgcgaaa gcagtcatgg
    23881 accgcatcct caaagatcat caaatcgtgg tcgatattcc ccacggagaa gcgtggctgc
    23941 gcgatgatga agagcgtccg atgattttga ttgcgggcgg caccgggttc tcttatgccc
    24001 gctcgatttt gctgacagcg ttggcgcgta acccaaaccg tgatatcacc atttactggg
    24061 gcgggcgtga agagcagcat ctgtatgatc tctgcgagct tgaggcgctt tcgttaaagc
    24121 accctggtct gcaagtggtg ccggtggttg aacaaccgga agcaggctgg cgtgggcgta
    24181 ctggtaccgt gttaacggcg gtattgcagg atcatggtac gctggcagag catgatatct
    24241 atattgccgg acgttttgag atggcgaaaa ttgcccgtga cctgttttgc agtgagcgta
    24301 atgcgcggga agatcgcctg tttggcgatg cgtttgcatt tatctgagat ataaaaaaac
    24361 ccgcccctga caggcgggaa gaacggcaac taaaacttta ttcagtggca tttagatcta
    24421 tgtcgcatcc ggcaattggt gcacgacgcc tgatgcgacg ctggcgcgtc ttatcaggcc
    24481 tacattggtg ccggatcggt agaccggata aggcgttcac gccgcatccg gcaagtggtt
    24541 aaacccgctc aaacaccgtc gcaataccct gacccagacc gatacacatc gtcgccagac
    24601 caaactgaac gtctttgcgt tccatcagat tcagcagcgt ggtgctgata cgcgcaccgg
    24661 aacagcccag cggatgaccc agcgcgatcg caccaccatt gaggttgatc ttctcgtcaa
    24721 tctgctccat tagcccgaga tctttaatgc acggcaggat ctgcgcggca aaggcttcgt
    24781 tcatctcaaa cacgccgata tcgctggcag aaagccccgc ttttttcagc gccagcttcg
    24841 aggccggaac cgggccgtaa cccataatcg atgggtcaca accaacgacc gccatcgaac
    24901 gcacacgagc gcgcggctta agacctaatt catgggcgcg gctttcactc atcaccagca
    24961 tggcagctgc gccatcggaa agtgcagaag atgtgcccgc cgttaccgta ccgtttactg
    25021 gatcaaacgc cggacgcagc gtggcgaggg cttccacggt ggtttccggg cgaatcactt
    25081 cgtcgtaatt aaactgcttc aggacgccgt cggcatcgtg accaccagtt gggatgattt
    25141 catttttaaa tgcggccgac tgcgtggcgg cccaggcgcg ggcgtgtgac cgcgcggcaa
    25201 aggcatcctg catttcacgg ctgataccgt gcatacgcgc cagcatttct gccgttaagc
    25261 ccatcatgcc cgccgctttg gcgacattgc ggctcaggcc ggggtgaaaa tcgacgccgt
    25321 gactcatcgg cacatggccc atatgctcca cgccgccaac cagacatgcc tgcgcatcgc
    25381 cagtcatgat cattcgtgct gcgtcatgca gtgcctgcat ggatgaacca cacaagcgat
    25441 tgacggtaac cgccgggaca gagtgcggca cttctgccag cagcgccgcg ttacgggcga
    25501 tattaaaacc ctgctccagc gtctgctgca cacaacccca gtaaatatcg tcgagggccg
    25561 ccgcttccag cgccgggtta cgcgccagca ggctacgcat taaatgagcg gagagatctt
    25621 ctgcacgcac gttacgaaaa gcaccgccct tcgaacggcc catcggggtg cgaattgcat
    25681 cgacaatgac aacctgttcc attgtgactc cttaagccgt tttcaggtcg ccaaccggac
    25741 gggctggctc aaccggagga tagtacggtt cgttatgacg cgctttatta cgcagacctt
    25801 tcggcacttc atacagcggg ccgaggtgct gatattgctg tgccatatcg aggtattttg
    25861 cgctaccgag ggtgtccagc cagcggaacg cgccgccgtg gaacggaggg aagcccaggc
    25921 cgtagaccag cgccatatcc gcttccgccg gagtggcgat aatgccttcc tccagacagc
    25981 gcaccacttc gttgaccatc gggatcatca tgcgggcgat aatctcttct tcgctgaaat
    26041 cgcgcttcgg ctggctcact tctgccagca ggtcttcaac ggcggcgtct tcttctttct
    26101 tcggcttacc tttgctgtct tctttataac gccagaaacc gaggccgttc ttctgaccaa
    26161 agcggttggc atcaaacagc gcgtcgatgg catcgcggta atctttctgc atccgctgcg
    26221 ggaagcctgc tgccatgaca gcctgagcgt gatgcgcggt atcaatgccc acaacgtcca
    26281 gcagatatgc cgggcccatc ggccagccaa actgtttttc catcactttg tcgatcttgc
    26341 ggaaatccgc gccgtcgcgc agcagctggc tgaaaccggc gaaatacggg aacagcacgc
    26401 ggttaacaaa gaagccgggg cagtcgttaa ccacaatcgg cgtcttgccc atcttgctcg
    26461 cccaggcgac aactttcgcg atggtttcgt cggagctttt ctcgccgcga ataatttcta
    26521 ccaacggcat tcggtggacc gggttaaaga agtgcatccc gcagaagttt tccgggcgtt
    26581 ccagcgcgtt ggccagttcg ctgataggaa tggttgaagt gttagacgcc agcacggtat
    26641 cctggcgtac tttttgttcg gtttctgcca gtacggcttt tttcactttc gggttttcaa
    26701 caaccgcttc taccacaata tccacgcggt caaatccggc gtagtcgagc gttgggtgga
    26761 ttgtggagat cacgccagcc agtttcagac catcgatctt gccgcgctca agctgcttgt
    26821 tcagcagttt cgcggcttcg gtcatgccga gggttaacga cttgtcgttg atatctttca
    26881 tgacaaccgg cacgcctttc cacgcagact ggtaagcgat gccgccgccc ataatgcctg
    26941 cacccagcac cgcggcctgt ttcggggttt caacgtcttt ggtgagtttc ttcgctttgc
    27001 cttttacata ttgatcgtta aggaaaatgc cgaccagtgc gcgggcttcg ttggtatgcg
    27061 ccagcgggac aaaacttttg ttttccaggt ttaaggcttc ttcacgacca aaacgggccg
    27121 cagcttcaat ggtttttact gcggtgatgg gggccggata atgtttcccc gctgtttgtg
    27181 cgaccatccc tttagcgatg gtgaagctca tggtggcttc aatcttgctc agttttagtg
    27241 gttccagctt cggctgacgt tttgctttcc agtcgaggtc gccgttaatg gcctggcgta
    27301 aaaccgcctt tgcgccttca accagttttt ctgctttgac tacgccatcc accagaccga
    27361 ttttcagcgc ctgatccgcg ccgacatctt taccggcggc aatgatttcc agcgcactgt
    27421 cagcgcccag catacgtggc atacgtacag aaccgccaaa gcccggcatg atgcccagtt
    27481 tggtttccgg cagaccgatg cgcagatccg gcgtcgccag acgataatcg gtcgccagca
    27541 cgcattcgca gccaccgccc agcgcatagc cgttgacggc agcaatggtc ggcaccggca
    27601 gatcttccag gcgattaaac acgctattgg caaagtgcag ccactgactt aactgttctt
    27661 caggaacgag gaacagggac aaaaattcgg tgatatcagc accgacgata aaggctgctt
    27721 tgttcgaacg cagcagcagc ccttttagat ctgattgctg ttccagcacg ccgatggcct
    27781 cgccgaggct ggcgacggtc gcagtgtcga gtttattaac tgaacctggg gcatcaaata
    27841 ccagttcggc aatgccatct tccagccagt caaggtacag ggtgtcgcct ttgtaaagca
    27901 tgtcagtctc ctgaatccgc aaggtgatct ggtcgtacca gatgcatcga agtgtgtatt
    27961 ttgtgttaaa aatatgcaaa taaaagatta aagaaatgcc gatctgatca cgctcggcag
    28021 aaatcacgct ctggatgaac gatgtgctaa gatgcggaga cttaaggtca aaaaaacaga
    28081 agggtaaaaa atggaatcac tggcctcact ctataaaaat catatagcta ccttacagga
    28141 acggactcgc gatgcgctgg cgcgcttcaa gctggatgcg ttacttattc actccggcga
    28201 actgttcaac gtttttctcg acgatcatcc ctatccgttt aaagtgaacc cgcaattcaa
    28261 agcgtgggtg ccggtaactc aggtgccaaa ctgctggctg ctggtggatg gcgtgaacaa
    28321 gccgaaactg tggttctatc tgccggttga ttactggcac aacgtcgaac cgctgccgac
    28381 ctctttctgg actgaagacg tggaagtgat cgcgctgccg aaagccgatg gcattggtag
    28441 cctgctgcct gctgcgcgcg ggaatatcgg ttatatcggt ccggtgccgg aacgtgcgct
    28501 gcaactgggt attgaggcca gcaatatcaa cccgaaaggg gtgatcgact acctgcatta
    28561 ctaccgctcc ttcaaaaccg agtacgaact ggcctgtatg cgtgaagcgc agaaaatggc
    28621 ggtcaacggt catcgcgcgg cagaagaagc gttccgttct ggcatgagcg agttcgatat
    28681 caacattgcc tatctgaccg cgaccggtca tcgtgatacc gacgtacctt acagcaacat
    28741 tgtggcgctc aacgaacacg ctgcggtgtt gcattacacc aaactggacc accaggcacc
    28801 ggaagagatg cgcagcttcc tgctggatgc cggggccgaa tataacggct atgcggctga
    28861 cctgacccgt acctggtcgg caaaaagtga caacgactac gcacagctgg tgaaagacgt
    28921 aaatgatgaa caactggcgc tgatcgctac catgaaagca ggcgtcagct atgtggatta
    28981 ccacatccag ttccatcagc gcatcgccaa actgctgcgt aaacatcaaa tcatcaccga
    29041 tatgagtgaa gaagcgatgg tcgaaaacga tctcaccgga ccgtttatgc cgcacggtat
    29101 tggccatccg ctgggcctgc aggtgcatga cgtagccggt tttatgcagg atgatagcgg
    29161 tacgcacctc gcggcaccgg caaaatatcc gtacctgcgc tgcacccgta ttctccagcc
    29221 gggcatggtg ttaaccatcg aaccgggtat ctacttcatt gaatcgctgc tggcaccgtg
    29281 gcgtgaaggg cagttcagca agcacttcaa ctggcagaaa attgaagcac tgaaaccgtt
    29341 cggcggcatt cgtatcgaag acaacgtggt gatccacgaa aacaacgtgg aaaacatgac
    29401 ccgggatctg aaactggcgt gatggaaagc tggttaattc ctgcggcacc ggtcacggtc
    29461 gttgaagaga tcaaaaaaag ccgcttcata acgatgttgg cgcataccga tggcgttgag
    29521 gcggcgaaag cgtttgttga atcggtgcgg gcagaacacc ccgatgcccg ccaccattgc
    29581 gtggcgtggg tggcgggtgc gccggatgat tctcaacagc tgggtttctc tgacgacggg
    29641 gagccggcgg gaacggcagg taaaccgatg ctcgcccagc taatgggcag cggcgtcggg
    29701 gaaattaccg ctgtggtagt gcgctactac ggcggcatat tgcttggcac cggtgggtta
    29761 gtgaaagcgt atggcggcgg cgtgaatcag gcgctgcgcc agctaacgac ccaacgcaag
    29821 acgccattaa ccgaatatac tttgcaatgt gaatatagtc agttaaccgg cattgaagcg
    29881 ttgctggggc agtgtgacgg caaaattatc aacagtgatt atcaggcatt cgttctgctg
    29941 cgggtggcgc ttccggcggc gaaagtggct gaattttccg caaagctggc ggattttagc
    30001 cgtggttcat tgcaattgtt agcgattgaa gaataatccc cacttcgttt tgcagaacta
    30061 aggaagcggc agagatgcat tttcgcgcca ttacccgaat cgttggacta ctggtcatct
    30121 tattttcagg gaccatgatt atccctgggc tggtagcact catctaccgg gatggagcgg
    30181 gccgcgcttt tacccagacc ttttttgtcg ccctcgccat tggttctatg ctgtggtggc
    30241 cgaaccgtaa agagaagggc gaactgaaat cccgtgaggg gtttctgata gtggtgctgt
    30301 tctggaccgt gctgggtagt gtcggtgcgc tcccttttat cttctcggaa agcccgaacc
    30361 tcacgattac cgatgcgttt tttgaatctt tctctggcct gaccaccacc ggggccacta
    30421 cgctggtggg gctggattcg ctccctcatg ccatcctctt ttatcgccag atgctgcaat
    30481 ggtttggcgg gatggggatc atcgtgttag cggttgcgat actgcctatc ctcggcgtgg
    30541 gtgggatgca gctctatcgc gcagaaatgc ccggcccgct gaaagataac aaaatgcgcc
    30601 cgcgaattgc ggaaacggcg aaaaccctgt ggttgattta tgtcttgctg accgtcgcct
    30661 gtgcgctggc gttgtggttt gccggaatgg atgcctttga tgccatcggc catagctttg
    30721 cgactatcgc tattggcggc ttctcgacac atgatgccag tatcggttat ttcgacagcc
    30781 cgactattaa cactatcatt gctatcttcc tgctgatctc cggctgtaac tacggtctgc
    30841 acttttcact gttaagtggg cgtagtctga aggtttattg gcgcgatccg gaatttcgca
    30901 tgtttatcgg cgtacagttt acgctggtgg ttatttgtac actcgtactg tggtttcata
    30961 atgtctacag ttcggcgctg atgacaatta accaggcgtt tttccaggtg gtatcgatgg
    31021 cgacaaccgc cgggtttacg actgacagca ttgcccgctg gccgctcttt ttgccggtac
    31081 tgcttttatg ttcagctttt atcggcggtt gcgccgggtc aacgggcggt ggcctgaaag
    31141 tgatccgcat cctgctgctg tttaagcagg ggaaccgtga gctgaaacga ctggtgcatc
    31201 cgaacgccgt ctatagcatt aagctgggga atcgcgcact gccggaacgt atcctcgaag
    31261 ccgtttgggg atttttctcc gcctatgcat tggtgtttat tgtcagtatg ctggcgatta
    31321 tcgccacggg cgtggatgac ttttctgcct ttgcctcagt tgttgcgaca ttgaataacc
    31381 tggggccagg gcttggcgtg gttgctgata actttaccag tatgaacccg gtggctaaat
    31441 ggatcctgat tgccaacatg ctgtttggtc gtctcgaggt ctttacattg ctggtgctct
    31501 ttaccccgac tttctggcgt gaatgatgga gtaatacgtg aaaacattaa ttcttttctc
    31561 aacaagggac ggacaaacgc gcgagattgc ctcctacctg gcttcggaac tgaaagaact
    31621 ggggatccag acggatgtcg ccaatgtgca ccgcattgaa gaaccacaat gggaaaatta
    31681 tgaccgtgtg gtcattggtg cttctattcg ctatggtcac taccactctg cgttccagga
    31741 atttgtcaaa aaacatgcga cgcgactgaa ttcgatgccg agcgcctttt attccgtaaa
    31801 cctggtggcg cgcaaaccgg agaagcgtac tccacagacc aacagctacg cgcggaagtt
    31861 tctgatgaac tcgcaatggc gtcccgatcg ctgcgcggtc attgccgggg cgctgcgtta
    31921 cccacgttat cgctggtacg accgttttat gatcaagctg attatgaaga tgtcaggcgg
    31981 tgaaacggat acgcgcaaag aagttgtcta taccgattgg gagcaggtgg cgaatttcgc
    32041 ccgagaaatc gcccatttaa ccgacaaacc gacgctgaaa taagcacaaa gaataaaaaa
    32101 tgcgcggtca gaaaattatt ttaaatttcc tcttgtcagg ccggaataac tccctataat
    32161 gcgccaccac tgacacggaa caacggcaaa cacgttgccg ggtcagcggg gttctcctga
    32221 gaatctcaac agagaaaagc aa
//
LOCUS       DAZGSP010000054        32164 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZGSP010000054 DAZGSP010000000
VERSION     DAZGSP010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.054"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(153..620)
                     /gene="yiaL"
                     /locus_tag="ACHK74_002752"
     CDS             complement(153..620)
                     /gene="yiaL"
                     /locus_tag="ACHK74_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFP0221541.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCIMQTASEIRKIVVKVALTALN"
     gene            complement(632..1630)
                     /gene="yiaK"
                     /locus_tag="ACHK74_002753"
     CDS             complement(632..1630)
                     /gene="yiaK"
                     /locus_tag="ACHK74_002753"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFP0221542.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGVSQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            1831..2679
                     /gene="yiaJ"
                     /locus_tag="ACHK74_002754"
     CDS             1831..2679
                     /gene="yiaJ"
                     /locus_tag="ACHK74_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFP0221543.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            2781..3254
                     /gene="ysaA"
                     /locus_tag="ACHK74_002755"
     CDS             2781..3254
                     /gene="ysaA"
                     /locus_tag="ACHK74_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP0221544.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(3406..4659)
                     /gene="avtA"
                     /locus_tag="ACHK74_002756"
     CDS             complement(3406..4659)
                     /gene="avtA"
                     /locus_tag="ACHK74_002756"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFP0221545.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(4837..6867)
                     /gene="malS"
                     /locus_tag="ACHK74_002757"
     CDS             complement(4837..6867)
                     /gene="malS"
                     /locus_tag="ACHK74_002757"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP0221546.1"
                     /translation="MKLATCFLTLLPGFAVAANWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASLVRECPKWDGLPLIVDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTPAPFNWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVNSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGDDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGESA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            7187..8011
                     /gene="bax"
                     /locus_tag="ACHK74_002758"
     CDS             7187..8011
                     /gene="bax"
                     /locus_tag="ACHK74_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFP0221547.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(8119..9297)
                     /gene="xylR"
                     /locus_tag="ACHK74_002759"
     CDS             complement(8119..9297)
                     /gene="xylR"
                     /locus_tag="ACHK74_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFP0221548.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(9375..10556)
                     /gene="xylH"
                     /locus_tag="ACHK74_002760"
     CDS             complement(9375..10556)
                     /gene="xylH"
                     /locus_tag="ACHK74_002760"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFP0221549.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIILTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(10534..12075)
                     /gene="xylG"
                     /locus_tag="ACHK74_002761"
     CDS             complement(10534..12075)
                     /gene="xylG"
                     /locus_tag="ACHK74_002761"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFP0221550.1"
                     /translation="MPYLLEMTNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(12153..13145)
                     /gene="xylF"
                     /locus_tag="ACHK74_002762"
     CDS             complement(12153..13145)
                     /gene="xylF"
                     /locus_tag="ACHK74_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0221551.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            13511..14833
                     /gene="xylA"
                     /locus_tag="ACHK74_002763"
     CDS             13511..14833
                     /gene="xylA"
                     /locus_tag="ACHK74_002763"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFP0221552.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            14905..16359
                     /gene="xylB"
                     /locus_tag="ACHK74_002764"
     CDS             14905..16359
                     /gene="xylB"
                     /locus_tag="ACHK74_002764"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFP0221553.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            16528..16869
                     /gene="yiaB"
                     /locus_tag="ACHK74_002765"
     CDS             16528..16869
                     /gene="yiaB"
                     /locus_tag="ACHK74_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFP0221554.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            16915..17352
                     /gene="yiaA"
                     /locus_tag="ACHK74_002766"
     CDS             16915..17352
                     /gene="yiaA"
                     /locus_tag="ACHK74_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFP0221555.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(17394..18389)
                     /gene="wecH"
                     /locus_tag="ACHK74_002767"
     CDS             complement(17394..18389)
                     /gene="wecH"
                     /locus_tag="ACHK74_002767"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFP0221556.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            18564..18863
                     /gene="ysaB"
                     /locus_tag="ACHK74_002768"
     CDS             18564..18863
                     /gene="ysaB"
                     /locus_tag="ACHK74_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFP0221557.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            18958..19869
                     /gene="glyQ"
                     /locus_tag="ACHK74_002769"
     CDS             18958..19869
                     /gene="glyQ"
                     /locus_tag="ACHK74_002769"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFP0221558.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            19879..21948
                     /gene="glyS"
                     /locus_tag="ACHK74_002770"
     CDS             19879..21948
                     /gene="glyS"
                     /locus_tag="ACHK74_002770"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFP0221559.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDVFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            22274..22426
                     /gene="hokA"
                     /locus_tag="ACHK74_002771"
     CDS             22274..22426
                     /gene="hokA"
                     /locus_tag="ACHK74_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFP0221560.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(22415..22474)
                     /locus_tag="ACHK74_002772"
     CDS             complement(22415..22474)
                     /locus_tag="ACHK74_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221561.1"
                     /translation="MRNAVRKAGITSRRRLLLF"
     gene            complement(22614..22826)
                     /gene="cspA"
                     /locus_tag="ACHK74_002773"
     CDS             complement(22614..22826)
                     /gene="cspA"
                     /locus_tag="ACHK74_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFP0221562.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(23107..23397)
                     /gene="yiaG"
                     /locus_tag="ACHK74_002774"
     CDS             complement(23107..23397)
                     /gene="yiaG"
                     /locus_tag="ACHK74_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFP0221563.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            23831..24541
                     /gene="yiaF"
                     /locus_tag="ACHK74_002775"
     CDS             23831..24541
                     /gene="yiaF"
                     /locus_tag="ACHK74_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFP0221564.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(24591..25565)
                     /gene="ghrB"
                     /locus_tag="ACHK74_002776"
     CDS             complement(24591..25565)
                     /gene="ghrB"
                     /locus_tag="ACHK74_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFP0221565.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(25669..26328)
                     /gene="yiaD"
                     /locus_tag="ACHK74_002777"
     CDS             complement(25669..26328)
                     /gene="yiaD"
                     /locus_tag="ACHK74_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFP0221566.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            26481..28814
                     /gene="bisC"
                     /locus_tag="ACHK74_002778"
     CDS             26481..28814
                     /gene="bisC"
                     /locus_tag="ACHK74_002778"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFP0221567.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGKTVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYIGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPCGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(28783..29223)
                     /gene="yiaC"
                     /locus_tag="ACHK74_002779"
     CDS             complement(28783..29223)
                     /gene="yiaC"
                     /locus_tag="ACHK74_002779"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP0221568.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(29220..29783)
                     /gene="tag"
                     /locus_tag="ACHK74_002780"
     CDS             complement(29220..29783)
                     /gene="tag"
                     /locus_tag="ACHK74_002780"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFP0221569.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            29941..30639
                     /gene="yhjY"
                     /locus_tag="ACHK74_002781"
     CDS             29941..30639
                     /gene="yhjY"
                     /locus_tag="ACHK74_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP0221570.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            30868..32070
                     /gene="yhjX"
                     /locus_tag="ACHK74_002782"
     CDS             30868..32070
                     /gene="yhjX"
                     /locus_tag="ACHK74_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0221571.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGTLLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         7895 a         7852 c         8187 g         8230 t
ORIGIN      
        1 ttaatcgcca gtattgcttc gagtattttt ttcatagcta ttccttgagg ctatgtctgt
       61 cataattcaa gttgcatgtg cagcaacctg aattattaat atagttagag caataacatt
      121 acccaggcgt tctggcgtag acgattattc gattaattca gcgccgttaa tgcgacttta
      181 accacaattt ttcgtatctc agaggctgtt tgcataatac aacctggtcg gtgaacatcc
      241 tgcggaaaga aaattgcgta gctgcccggt atcatttcta taaatgattc atgttcactg
      301 tcatgataaa aaataatatc gcgctgctct aatagtgatt cgctgacttt attatttccc
      361 gtatcaatag caatgccgat tttctcttca ccccacgcca gaaactgaat atcgatatac
      421 cgacgatgca cttccggacg gttttccacc gcttcgcgtg tggttaaatc gataatttgc
      481 gcataaatat ttttgccgtc gatttcgaca acgcccggct ccagggcgtt gaaatcggta
      541 gcgcgcagaa aatcgagcgc cttttcaata gcggcgggca aacggcacgg gtttggctgc
      601 gcgatatgtc caaaaatcat ggcttatctc cttataacgc ctggattttg gcccacacgc
      661 tgtcatcaac agtgatgccg ttacggcggt tttcggccag cagggtagta aattcatggc
      721 cgggtaagcg gatggcctgg ttttcgtcag cgcgttcagc ggtagtaacg taatccatga
      781 tgcgttgcag cttggcatcg cgggtggggc cgtcgatcaa tttatccact tcgatggcga
      841 taaagatttg cgaaacgccg tattcgtcgc tgttgtcctg ggtgacttcg gcaaccgatg
      901 cgccgtcgga aaggagagta gcgatcatat ccagcacaat cgacatgcca gaacctttcc
      961 agtagcccat cggcaaaatg cggcgattct tctcgataac gccaggttct ttggtcaaat
     1021 tgccctcatc atcaaagcca ccatcgaccg ggagctgacg gcccgccagg cggttaactt
     1081 ctaacatgcc gtaggaaaac atcgacatcg acatatcgac catggtgatc ggcgtggaag
     1141 gaatggcgac gatcagcggg ttagtgccga tgcgacactc ttttgcgccc cacggcggca
     1201 ttacggcgat ggagttggtc cagcaaatgc caatatagcc tttttccgcc gcctgccagc
     1261 cgtagctgcc accgcgcatc cagtggttgg cattacgtag tgccaccaga ccaataccgt
     1321 gatcggcagc cagttcaatg gcgcgatcca tcatcttttt cgccgtcaga ttgccgatcg
     1381 aacgctgggc gtcccactgt tcaattgcgc cgaggctggt tatacgtttg ggttgggcat
     1441 caggaatgat atcgccgttt tccagctgtt gaatgaaacg agggaaacga ttaacgccgt
     1501 gagaataaac gccggattcg gtggtgcggg cgaacatctc tgcacaggcg tcagccgttt
     1561 cgctgtcaac gccgcgtgaa attaagaccc gattaaaggc tgcttttaac tgctcaaatg
     1621 tcactttcat cccaggcttc cttgtttttt tgatcgcttt tttggcttaa aatttcaata
     1681 tgcgaaactt gatttcaaat atatcgatcg ctttttaaca aagcaatctg attgatgaat
     1741 ttcaaaaaac ttaaaaatca agtggttata aattatctgt cgagatcgtg aactacggca
     1801 cactttgcgc taccatcagg acgcgacaaa atggggaaag aagtgatggg aaaaaaagag
     1861 aacgagatgg cgcaggaaaa agagcgtcca gccggaagcc agagtctgtt tcgcgggttg
     1921 atgctgattg agattttgag caactatcca aacggttgtc cgttggcgca tctttcggag
     1981 ctggctggtt taaataagag taccgtccat cgcttattgc agggattaca gtcctgcggc
     2041 tatgtgacca ccgcgcccgc cgcagggagt taccgcctga ctaccaaatt tattgccgtc
     2101 gggcagaagg cgctgtcatc gctgaatatc attcatattg ccgctccgca tcttgaggca
     2161 ctgaacatcg ccactggtga aaccattaac ttctccagcc gcgaagacga tcacgctatt
     2221 ttgatttata agctggaacc cacaaccggg atgctgcgaa cccgtgccta tattggccag
     2281 catatgccgc tctactgttc cgcaatgggc aagatctaca tggcgtttgg tcacccggac
     2341 tacgtgaagt catactggga aagccatcag catgagatcc agccgttaac ccgcaatacc
     2401 attaccgagc tgcccgcgat gttcgacgaa ctggcgcaca ttcgtgaaag cggagcggcg
     2461 atggacagag aagaaaacga actcggcgtc tcctgtattg ctgttccggt gtttgatatt
     2521 catgggcggg tgccgtacgc cgtgtcgatt tcgctttcga catcacgtct gaaacaggtg
     2581 ggggagaaaa atctcctgaa accactgcgt gaaaccgcgc aggctatttc taatgaactg
     2641 ggatttactg tcagggatga cctgggcgcg atcacataac gctttggaca agtgccaaaa
     2701 ctttaacatt tccttcgttg gatcaaagca gtagggacgc gctctctggc actctgctgt
     2761 tttagtgcaa aggagtgatc atgaaccggt ttattattgc ggatgcgacg aaatgtatcg
     2821 gttgccgtac ctgtgaagtg gcttgcgcag tgtcgcatca ggagaatcag gattgcgctg
     2881 cgttgtcacc agacgagttt atttcccgta ttcgtgtcat taaagaccac agctggacca
     2941 cggcagtagc ctgtcatcag tgtgaagatg caccgtgcgc gaatgtctgc cctgttgacg
     3001 cgataagccg cgaacatggg catattttcg ttgaacaaac acgttgcatt ggctgtaaaa
     3061 gctgtatgtt ggcttgcccg tttggtgcga tggaggtcgt ttcttcgcgc aaaaaggcga
     3121 gggcgattaa gtgcgatctg tgctggcatc gggagacggg accggcctgt gttgaagcct
     3181 gcccgacaaa ggcgttgcag tgcatggatg tcgagaaagt gcagcggcat cggctacggc
     3241 agcagcctgt ttgaaacgtt tttccggcgt ttctcaacgc cggaatagtg cgctttgttt
     3301 atgccagatg cggcgtaaac gccttatccg gcctacaaat tcttccaaat tcaatatatt
     3361 gcagaaatca tgtaggcctg ataagcgtag cgcatcaggc aatttttagt gactttcagc
     3421 ccaggctctt tctatctctt ccgccagaat cttcaccccc gcctcaattt tctccggctc
     3481 tggtacgtag ttcatgcgca tacattgatg cgtatgcggc cacggtttat ccagccccgg
     3541 gaagaagttg tgccccggca ccatcagcac gccgcgtgct ttcaggcgct ggtagagttg
     3601 ctcggtcgta atgggcaaat ccttaaacca tagccagagg aaaatggctc cttccggttt
     3661 atgaatcagg cagcgatctt ccggtaaata gcggcgaatg atggcgatgg tttcctgaac
     3721 acgctggtag taaaacggtt tgatgactgt ttcagacagg cgcagcagat cgttacgctt
     3781 aatcatttca cacatcatcg ccggaccaat accgccaggt gccaggctga taatgccgtt
     3841 catattggtg atggcggtga tgattttttc atttgcgatg ataatgccgc agcgggagcc
     3901 aggtagaccc agcttggaaa gactcatgca cagcacgata ttcggattcc acagcgggcg
     3961 cgcttcactg aagatgatac ccgggaacgg gacgccataa gcgttatcaa tcaccagtgg
     4021 aataccgtgc tgattcgcca gcgcgtcaag cttcagtaac tcttcgtcag taatcacatt
     4081 gcctgttgga ttcgtcggcc gggagacgca aatcatcccg gtttcttcgc caatatgcag
     4141 atgctcaaaa tcgacgtgat atttgaactg gccttccggc agcagttcaa tattcggacg
     4201 cgcagagaca aacagatctt cttccagtcc ggcgtcagcg tagccaatgt attccggtgc
     4261 cagcggaaac agcacttttt tgacccgacc atcggcacgg cgtccggcaa acaggttaaa
     4321 taagtagaaa aacgcgctct ggctgccgtt tgttagtgca atattctgtg gttcgatatc
     4381 ccaacccaac ttctcgcgca gcattccggc aagcagtgtg agtagctccg ttttcccctg
     4441 tggaccgtcg tagttacaca gtgcatcagt cgctttgcca ctttccagca tgtcggtcag
     4501 tagcgtctgg aagtagtcct gcatttccgg gatctgcgcc ggattaccgc cgccgagcat
     4561 aatcgcgcca ggcgtgcgta aaccgtcgtt cagatcttcc atcagcagcg taatgccgga
     4621 gtggcgggta aatttgtcac caaaaaggga gaatgtcata gcgggatatc tgtcgaactc
     4681 tgtagcaagg aaggtaacaa taacgctaca tttgctgggg tgcaaatcgg tctgttgaag
     4741 ggtgagtggt gctttatcct gcaactctga ttagggctga cattttatcc tggttggcgg
     4801 cttcccgagg tactgacgaa cgggaagccg gaaaagtcac tgttgcccag cccagatgac
     4861 cagcacttta tcgtcgccat gctcacgaac aaagccgtag ccctgcttca gcgaaagtgt
     4921 cgtttgtttg cccgcgccaa ttgcgggatg gcgggcgcgg aactggctga ttttctgcca
     4981 gtgtgcgacg ctggcggcag attcaccgct aacatcctgc cagttcatat ccgaacgtgt
     5041 accttgcagc ggatcagaac cggtagggcc gaacggacgc gaggattcat cgccataaaa
     5101 gatttgtacc gcgcctggcg ctaataataa taactctgct gctttgtcgt ccccttcacg
     5161 gaacaggcgg gtatcatgcg acgataggta gctcaacacg ttgaaatcct gcaatttctc
     5221 cgccatttgc tgccaggtcg tatccatctg cgccagacaa tcgactgctt tcgccgcctg
     5281 ctcctgataa tcgaaattga tcatcgcatc gaagccgtgg cgatagtagt cactttgcat
     5341 cacgccgtgg ccccaggctt caccggtcat ccagaaaggt ttgtcatcta atgctttgtc
     5401 ggggttagct tttttccatt cgcgaagcgc ggcgctggct tcggttttca gttgctgcca
     5461 ggcgggcaac tcaacatgtt tggcggtatc gacccgaaaa ccatcaatcc catagtcgcg
     5521 gacccactga cttaaccagt gggttaagta atcgcgaggt gtaaagcctt cgatgacttt
     5581 agcgtgtgta tccgttttgt ttttatagaa caccggcaga ccagaagcgg tagttgattc
     5641 ggttttgata tccggcaaaa aggccagcga catagtgaga tcgtcaaatc caggattgtc
     5701 gtaatcgccg atatccgttc tgatccagtt ttttccccac catttatccc agcctgtttt
     5761 gtcgctgaaa ttaatgtaat cgttaaagct atgccaggtt tgcccggcgg caggtttcca
     5821 gtcgctccag cgttcaccca gcgttttttt cacttcgtca ccagaaagat ataacgcgcc
     5881 aaactgatac tcctgcatat ccgccagcgt ggcatagccg gtgtggttca tcacgacatc
     5941 aaagagaata cgaataccgc gctgatgtgc gctattaacc agcgtccgta gatcggcttc
     6001 gttgcccata ttggcatcaa gattcgtcca gtcctgtgtg taataaccgt ggtaggcata
     6061 atgcgggaaa tcgccttttg taccgccgcc gacccagccg tgaatttgct caaatggggc
     6121 gcttatccat aaagcattaa cgcccaactg ctggaggtaa tccagtttgt tggtcaggcc
     6181 gcgtaaatcg ccgccgtgaa aagtgccaat ttccgccata ccgtctttat gacgtccgta
     6241 actctggtca ttactgggat cgccgttttc gaaacggtct gtcagtacaa agtaaaccgt
     6301 ggcgttatgc cagttgaaag gggcaggggt gtcagtttcg gcccgttcca gcaggagtaa
     6361 accgttgctg gtggcagcgg gttgtaaggt tatttgaccg ttcttcacta tcgcaatttg
     6421 ctggctgtaa taatcccgta cggcggctcc ttccgggaaa gtggcgctga catccactat
     6481 gagcggtaat ccatcccatt tcgggcattc acggaccagg cttgctaccg gttcggcggc
     6541 gttctggatg gaaatcatca atgttggcgt accggagcgg gtgtctattt gcagcgtata
     6601 ttcgccgtcc ctgaacaatc gccattgagg cggcgtgttg ctacaaggtt gcagggaaag
     6661 catctgattg agttttatcg catctgcagg ctgccagcac tgttggtcaa aatttagcgt
     6721 tagtggacgc gtacctttgg gcaactgcgc gtggctgaca aatgttcctg tcccctgttc
     6781 gctaaaggcg ggaaaccccg gagaagtcca gttggcggca acggcgaagc caggaaggag
     6841 tgtcagaaaa caggtggcga gtttcatggc ggatgagtcc ttattgctgc gatttcagac
     6901 agtttgccag cgataagcca gacaaaactc atccctgagc ggtaagtcca caggatgaga
     6961 agcaagggtg agcgatcgcg cgcaaaaaac ggctgaattt tgcgataaac cccacatttt
     7021 ctgcgattta gcgccaatct gaatcgttaa ctcgtgacat agtttcagat ttggactatt
     7081 ccttggggtg ctcttgacag ctatttttga tatgatttga gattcaactc tcaaatttgt
     7141 gaaaaatata aggtgttgga atgattaaat ccgaccagga gacctgatga ttttgactcc
     7201 catacgacga tatggggcga tgattcttat gttactcact ctggtgtttt cgagtgaggt
     7261 gttagcgaag acgcacacaa caacagcgag tcaaaagtcc cacttaacta aagctagtaa
     7321 taaacaggta agcagtaaac aagagtattc tcgcaatagt gcaaagagta gttcacttcc
     7381 tgatttgcga aaataccctt ccggaacacc aaggaaaaag gcgtttctcc ggactgtaat
     7441 gccttacatt accagccaaa atgcggccat tactgcggaa cgtaactggc tgatttcaaa
     7501 acagtatcag ggccaatggt cacctgctga gcgtgcgcgt ctgaaagaca tcgccaaacg
     7561 ctacaaggtg aagtggtctg gtaatacgcg aaaaatcccg tggaatacct tgcttgaacg
     7621 cgtagacatt atccctacca gtatggtggc gacgatggct gcagcagaaa gcggttgggg
     7681 aacgtcgaag ctggcgcgca acaacaacaa tcttttcggc atgaaatgca tgaaaggacg
     7741 ttgtaccaat gcgccgggta aagtgaaagg gtactcacag tttagttctg tcaaagaatc
     7801 ggtgagcgcc tatgtcacta acctcaatac gcatccggct tactcttcgt tccgtaaatc
     7861 gcgtgcgcag ctgcgtaaag cggatcagga agtgactgcc acagcgatga ttcacaagct
     7921 gaagggctac tcgaccaaag ggaagagtta taacaactac ctgttcgcaa tgtaccagga
     7981 taaccaacgg ttaatcgcgg cgcatatgta attgtatttc ctcgagcctt atccgacttg
     8041 tcggtcggat aaggcttttg atcgcatcag gtggttgtgc tgagttacct gatgcgacgc
     8101 cgacaattct catcatcgct acaacatgac ctcgctattt acatcgcgat actcttttgg
     8161 cgtcgtgtca tatgcttttt taaaaacaga gtagaaatat tgcagcgatg gataaccgca
     8221 catttgcgat atctcattga tcgacaaggt ggttgaaatc agcagactgc gcgctttctc
     8281 cagcttctcg gcatgaatca tggcatggat agtttcaccc acctcttctt taaaacgctt
     8341 ctcaagattg gagcgcgaga tcccgaccgc atccagtacc tgatccactt taatcccttt
     8401 acaggcgtga ttacgaatgt aatgcatggc ctgaataacg gcgggatcgg tcagcgagcg
     8461 ataatctgtt gagcgccgtt caatgacgcg aactggtggg accaaaattc gctgtagcgg
     8521 catttcttct ttatctaata atcgatgcaa cagttttgcc gcctgatagc ccatttgccg
     8581 cgcgccctga gcgaccgaag aaagggcaac acgcgacaga tagcgggtca gttcttcgtt
     8641 atcgatgcca atcacgcata atttttccgg tacgggaata tgtagatgtt cacatacttg
     8701 cagaatatgc cgcgcccggg cgtcagtaac ggcaataatc ccggtttgcg gtggcagcgt
     8761 ttgtagccag tctgccagcc gattttgcgc atgttgccag ttctctggcg cggtttctaa
     8821 cccctgataa accactccgc gatacttttc ttcggcgaca agctgacgaa atgcatattc
     8881 gcgttcagtg gcccaacgtt taccgcttga ttccggaaga ccataaaaag caaagcggtt
     8941 aacgcctttc tcttttaaat gcaaaaatgc gctttcaacc agcgcatagt tatcggtggc
     9001 aatgtaatga acgggtgggt aactttcggc aaggtgatac gagccgccaa ccccaacaat
     9061 ggggacgtcg acatcagcca gcgcttgctc gatttgtttg tcgtcgaagt cggcaatgac
     9121 gccatctcct aaccagtcct tgattttatc aatgcgggcg cggaaatctt cttcaatgaa
     9181 aatatcccat tccgattgtg acgcctgtaa atattcccct acgccttcta ctacctgccg
     9241 gtcataggct ttattggcat tgaacagtaa tgtgatgcgg tgacgtttag taaacatggt
     9301 tcttttcctg ctgaatcatg caaaaactca aaaccggtaa tacgtaaccg gctttgagaa
     9361 aatttttatc aaaatcaaga acggcgtttg gttgcggagt ccatccatac tgccagcaac
     9421 agaatcgcac ctttaacgat atactgccag aaggtcggta catccatcat actcatgccg
     9481 ttatccagtg aagccatgat aaatgccccc attaccgctc cagccacgct cccgacaccg
     9541 ccagccaggc tggtaccgcc aatcacgcat gctgcaattg cgtccagctc ggcgatattc
     9601 cccgcagaag gtgaaccagc gccaagtcga gaactgagga ttaatccggc gatggctacc
     9661 attaatccgt taatcgcgaa cacggcaagt ttggtgcgtt caacgttaat cccggagaga
     9721 cgtgctgctt ccagattgcc gccgatggca taaatgcgtc gtccaaatgc cgtccgcgtt
     9781 gccataaaca ttccgccgag taacagcaac gtcagcagca gaacaggagt gggaacgcca
     9841 cggtaatcat tcaacagcca gattgcgcct aatacgatga tagcggttaa agcctggcga
     9901 ccgactactg cggtagaggc cggagactgt aaacccaaag cctgacggcg cattcttccg
     9961 cgccattgcc aaccaacaaa agccattaag ccaagcgcgc caatgatgaa gccggtactg
    10021 gcggggagat agctttgtcc aatttgtgac atcgcggcgc tggtggggga tacagtcgtg
    10081 ccgttggtga tgccaatgag tatgccgcga aatgccaaca tgcccgcgag ggtgacaata
    10141 aatgaaggga ctttacggta cgcgacccac catccgttcc acgcaccgag aagcagtccc
    10201 agaaccaacg tcagaatgat ggtaagtggc aaaggccagc ctaaccagac gtcacaaatc
    10261 gccgcgacgc cacctaatag ccccatcatt gagccgacgg aaaggtcgat ttcagcagaa
    10321 attatgacga acaccattcc taccgcgagg atgccggtaa tcgcggtctg gcgtaacagg
    10381 ttggagacgt tacgggcgct taagtaggca ccatcggtgg tccaggtaaa gaacagcatg
    10441 attgcgatga tagctgcaat catcacgaag acctgcaaat tcagtgactt cagcccagaa
    10501 aaaccaccgg atgtcggtac ggccaatttc acttcagacg gattgctttt cgacatgatg
    10561 ttcgctcctc aatgcggctt ccatcacctg ctcctgagtc aggttatgat ttatcaggtt
    10621 ggcttttagt ttcccttcat gcatcaccag tacccgatcg ctaaggccga gcacttcagg
    10681 taattcggaa gagatgacaa taacggcaat accctgctgg acgagttggt taattaattt
    10741 gtagatctcg tatttcgcgc caatatcgat acccctggta ggttcatcaa gaatgaggat
    10801 gcgcgggtta agtaacagac agcgagcgag gatcgctttt tgctgattgc cgccgctcaa
    10861 acgtccaata gcaaggtcgg gggacgacgt tttaactttg agttgctgaa ttgattccag
    10921 aatacatttt tgctctgccg cgtcatcaag ctggctaatg ccaccggtaa atttattgag
    10981 tgcggcgagg gtaatatttt taccaaccgc cattaccgga acgatgccgt cgcgctttct
    11041 gtcttcgggt accatcgcaa tcccctgggc gatggcttgc tgacagttac gaatatctac
    11101 ctgtttgcca tcaatataaa tttttccttc ccattgtccg ggccacacgc caaacaggca
    11161 ctgaatggtc tcggtacgtc cggcaccaac gagtccggca atacccagta tttcgccacg
    11221 tttcagggaa aacgagacat cattaactct tttaatatga cgattaaccg gatgccatgc
    11281 cgtcagatgt tcaatacgta atatttcatc tccggtggta tgtggttcat tagggtaaag
    11341 cgcggttaac tctcgcccga ccatcatggt gataatatcg tcttcactca ttccggcagc
    11401 atcacgcgta ccaatgtgct gaccgtcgcg aataacgcaa atcgtatcgg aaatcgcttt
    11461 gacttcgttg agtttgtgcg aaatataaat acaggcgata ccgtgctgtt gtagatcgcg
    11521 aataatatcc agtaaaaccg acgtttcctg ctcagttaat gaggctgtcg gttcatcgag
    11581 aattaacaag cgcacctgtt tattaagtgc cttggctatt tcaaccagtt gttgttgccc
    11641 aagccctaaa tctcccacgc gggtatctgg tgaaatggat aaactgacct gtgcgagcag
    11701 cttctgacag cgtagcgtca tcaggtcata atccataata ccattgtggg ttatttcgtt
    11761 acccaggaag atattttcca gtacggtcaa ttctttcacc agggccaatt cctggtgaat
    11821 gatggcgata cctttgcgtt cggtatcgcg gatgtgactc gcctgaatct cttctcccgc
    11881 aaaaataatt tcgccttcgt aggagccatg gggataaata ccacacagca ctttcatcag
    11941 cgttgattta ccagacccat tttccccaca gagtgagacg atttcgccag cattcaaccg
    12001 caagctgacg ttatcaatcg ccttcacact gccgaaggtt ttggtaatgt tcgtcatttc
    12061 aagtagataa ggcataacga ctccacctaa gccaattaat tcacgcggca tggagagaaa
    12121 tcacgccccc gctccgcgcc ggggcgtaac gcttacagct cgctctcttt gtggaatccg
    12181 tctttaatta ccgtatcttt aatgttgttt ttattcacat cgatcggtgt caggaggcgg
    12241 gaggggacat ctttcaggcc attattcagt gaggtatctg cttttggctc ctgaccattg
    12301 cccaattcaa cggcaatttc tgcggcagta tttgccaaca acgtaatagg tttatacacc
    12361 gtcatagttt gcgtaccggc agcaatacgc ttaatacctg cgagatccgc atcctggcca
    12421 gaaattgcta ctttccctga taaaccttgc gcgcttaatg cctgaattgc cccacctgcg
    12481 gtggcatcgt ttgaggcaac tacagcatca attttgttat tattggcggt tagcgcgttt
    12541 tccataattt tcaatgcgtt ttccggtaac cagccatcaa cccattggtc accaacgact
    12601 ttaatttttc cggaatcaac gtaaggtttt aacactttca tttgtccggc gcggaacagc
    12661 ttggcgttgt tatctaccgg cgagccgccc atcaggaagt aattaccttg cggaacaata
    12721 tcgaccaggg cttttgcctg cagttcaccg actttttcgt tatcgaaaga aatataaaaa
    12781 tcgatatccg catcgttaat catacgatcg taagctaata ctttaatgcc ttcttgtttg
    12841 gcttctttta caacgttact taatacctga ccgttatacg gaataatgac aagaacatcg
    12901 acaccccggt taatcatgtt ttcaatctgc gacatttgtg tttcttcatt gccatttgca
    12961 gactgtacaa atactttcgc gccgagagat tctgcctttt tcacaaagat atctcgatct
    13021 ttttgccagc gttcaagacg gagatcatca atcgccatac ctattttgac ttctttggcg
    13081 tgtgcagcaa cgttggtaag caggagtgag gtgcaaaggg tgagtagaat gttctttatt
    13141 ttcatggtgt agggccttct gtagttagag gacagcttta ataagtaaca atcaccgcga
    13201 taaacgtaac caatttttag caactaaaca ggggaaaaca attacagatt tttatctttc
    13261 gattacgatt tttggtttat ttcttgattt atgaccgaga tcttactttt gttgcgcaat
    13321 tgtacttatt gcatttttct cttcgaggaa ttacccagtt tcatcattcc attttatttt
    13381 gcgagcgagc gcacacttgt gaattatctc aatagcagtg tgaaataaca taattgagca
    13441 actgaaaggg agtgcccaat attacgacat catccatcac ccgcggcatt acctgattat
    13501 ggagttcaat atgcaagcct attttgacca gctcgatcgc gttcgttatg aaggctcaaa
    13561 atcctcaaac ccgttagcat tccgtcacta caatcccgac gaactggtgt tgggtaagcg
    13621 tatggaagag cacttgcgat ttgccgcctg ctactggcac accttctgct ggaacggggc
    13681 ggacatgttt ggtgtggggg cgtttaatcg tccgtggcag cagcctggtg aggcactggc
    13741 gttggcgaag cgtaaagcag atgtcgcatt tgagtttttc cacaagttac atgtgccatt
    13801 ttattgcttc cacgatgtgg atgtttctcc tgagggcgcg tcgttaaaag agtacatcaa
    13861 taattttgcg caaatggttg atgtcctggc aggcaagcaa gaagagagcg gcgtgaagct
    13921 gctgtgggga accgccaact gctttacaaa ccctcgctac ggcgcgggtg cggcgacgaa
    13981 cccagatcct gaagtcttca gctgggcggc aacgcaagtt gttacagcga tggaagcaac
    14041 ccataaattg ggcggtgaaa actatgtcct gtggggtggt cgtgaaggtt acgaaacgct
    14101 gttaaatacc gacttgcgtc aggagcgtga acaactgggc cgctttatgc agatggtggt
    14161 tgagcataaa cataaaatcg gcttccaggg cacgttgctt atcgaaccga aaccgcaaga
    14221 accgactaaa catcaatatg attacgatgc cgcgacggtc tatggcttcc tgaaacagtt
    14281 tggtctggaa aaagagatta aactgaacat tgaagctaac cacgcgacgc tggcaggtca
    14341 ctctttccat catgaaattg ccaccgccat tgcgcttggc ctgttcggtt ctgtcgacgc
    14401 caaccgtggc gatgcgcaac tgggctggga caccgaccag ttcccgaaca gtgtggaaga
    14461 gaatgcgctg gtgatgtatg aaattctcaa agcaggcggt ttcaccaccg gtggtctgaa
    14521 cttcgatgcc aaagtacgtc gtcaaagtac tgataaatat gatctgtttt acggtcatat
    14581 cggcgcgatg gatacgatgg cactggcgct gaaaattgca gcgcgcatga ttgaagatgg
    14641 cgagctggat aaacgcatcg cgcagcgtta ttccggctgg aatagcgaat tgggccagca
    14701 aatcctgaaa ggccaaatgt cactggcaga tttagccaaa tatgctcagg aacataattt
    14761 gtctccggtg catcagagtg gtcgccagga gcaactggaa aatctggtaa atcattatct
    14821 gttcgacaaa taacggctaa ctgtgcagtc cgttggcccg gttatcggta gcgataccgg
    14881 gcattttttt aaggaacgat cgatatgtat atcgggatag atcttggcac ctcgggcgta
    14941 aaagttattt tgctcaacga gcagggtgag gtggttgctt cgcaaacgga aaagctgacc
    15001 gtttcgcgcc cgcatccact ctggtcggaa caagacccgg aacagtggtg gcaggcaact
    15061 gatcgcgcaa tgaaagctct gggcgatcag cattctctgc aggacgttaa agcattgggt
    15121 attgccggcc agatgcatgg agcaacctta ctggatgctc aacaacgggt attgcgccct
    15181 gccattttgt ggaacgacgg gcgctgtgcg caagagtgca ctttgctgga agcgagagtt
    15241 ccgcaatcac gagtgattac cggcaacctg atgatgcccg gatttactgc gcctaaattg
    15301 ctatgggttc agcggcatga gccggagata ttccgtcaaa tcgacaaagt attattaccg
    15361 aaagattact tgcgtctgcg tatgacgggg gagtttgcca gcgatatgtc tgacgcagct
    15421 ggcaccatgt ggctggatgt cgcaaagcgt gactggagtg acgtcatgct gcaggcttgc
    15481 gacttatctc gtgaccagat gcccgcatta tatgaaggca gcgaaattac tggtgctttg
    15541 ttacctgaag ttgcgaaagc gtggggtatg gcgacggtgc cagttgtcgc aggcggtggc
    15601 gacaatgcag ctggtgcagt tggtgtggga atggttgatg ctaatcaggc aatgttatcg
    15661 ctggggacgt cgggggtcta ttttgctgtc agcgaagggt tcttaagcaa gccagaaagc
    15721 gctgtacaca gcttttgcca tgcgctaccg cagcgttggc atttaatgtc tgtaatgctg
    15781 agtgcagcat cgtgtctgga ttgggtcgcg aaattaaccg gcctgtgcaa tgtcccagct
    15841 ttaatcgctg cagctcaaca ggctgatgaa agtgccgagc cagtttggtt tctgccttat
    15901 ctttccggcg agcgtacacc acacaataat ccccaggcga agggggtttt ctttggtttg
    15961 actcatcaac atggccccaa tgaactggcg cgagcagtgc tggaaggcgt gggttatgcg
    16021 ctggcagatg gcatggatgt cgtgcatgcc tgcggcatta aaccgcaaag tgttacgttg
    16081 attggtggcg gggcgcgtag tgagtactgg cgtcagatgc tggcggatat cagcggtcag
    16141 cagctcgatt accgtacggg gggggatgtg gggccagcac tgggcgcagc aaggctggcg
    16201 cagattgcgg cgaatccaga gaaatcgctc attgagttgt tgccgcagct accgttagaa
    16261 cagtcgcatc taccagatgc gcagcgttat gccgcttatc agccacgacg tgaaacgttc
    16321 cgtcgcctct atcagcaact tctgccatta atggcgtaaa cgttatcccc tgcctgaccg
    16381 ggtgggggat aattcacatc tatatatctc actaattaat taatatttag tatgaattta
    16441 ttctgaaaat catttgttaa tggcattttt cagttttgtc tttcgttggt tactcgtaat
    16501 gtatcgctgg tagatatgga gattgttatg aaaacctcaa aaactgtggc aaaactatta
    16561 tttgttgtcg gggcgctggt ttatctggtt gggctatgga tctcatgccc attgctaagt
    16621 ggaaaaggct attttcttgg cgtgttaatg acagcaactt ttggtaacta tgcatatctt
    16681 cgcgcagaaa aactcgggca actggataat ttttttaccc atatctgcca gttagttgcg
    16741 ttaatcacta tcggtctctt gtttatcggt gttttaaatg cacctatcaa tgcttatgaa
    16801 atggtgatct accccatcgc cttttttgtc tgcttgtttg gtcaaatgcg tttgtttcgc
    16861 tcggtatgag caacataaag ctcttacaca ttcaggaatg aaaggaatac tgtgatggac
    16921 aacaaaatat caacctattc accggccttt agtattgtgt catggatagc tctcgttggt
    16981 ggtatcgtta cctatctgtt agggctatgg aatgcagaga tgcagttaaa tgaaaaaggg
    17041 tattattttg ccgtactggt gttaggactg ttttctgctg cgtcttatca aaagaccgtt
    17101 cgggacaagt atgaaggcat accgaccact tccatttatt atatgacctg cctgactgtc
    17161 tttattatct ctgttgcatt actgatggta ggtctgtgga atgcgacatt attactcagt
    17221 gaaaaaggtt tttatggact ggctttcttc ttaagcttgt ttggtgcagt agcggtgcag
    17281 aagaatattc gtgataccgg aataaacccg ccaaaagaaa cacaggttac ccaggaagaa
    17341 tacagcgaat aattcacgta agcccggtca gtccaatgtg aacgggcttt tacttaactc
    17401 actaatctgt ttctgtcgat tcgttgtacc agcatagaaa gtaacaaact cgctgccaac
    17461 gtcgcgcaaa agatccaaat aatatccagt attggccaat ttttaagctc aattccccgg
    17521 gtgcgcagcg catggataat caaggcgtgg aatccgtata tacccaatga atggcgggag
    17581 attaagccaa gtccgcgaat ggtacgcgta tccagcgtgt ttttaaccag agtcaatagc
    17641 gcgattgcgc agataaaaac catcggccca cagtaaagat accaggtatc ggcaaaattt
    17701 ccgcgccact gcaattcata taatgtcccg cgagagataa taaaaacccc cgtcgcaaac
    17761 agcgcggcgc tcacccacga cagtgcttta tgctgtgtgt ccatcatccc tatagcgcgg
    17821 cccaacatgc catacagaat gtagtaaaaa gtatcgccat tgatatataa gttaattggc
    17881 agccattcaa aaccgtcaat tttctgcggc actgtgtttg ggttagcgat aatgccaatt
    17941 accaccatta gcaccagcaa catttttccg ccgacgttct tcacctgaat cagcggtgaa
    18001 accagataaa tcaccgcaat cgcgaagaaa aaccacaagt ggtaaaacac tggcttttgc
    18061 agcaggtttt tcagcgctaa ctccatattg atggaggtaa acagcgcaat gtagagcagt
    18121 gcgattgcgc tataaaaaat cagacataag ccgatacgca agaaatggcg cggctgggcg
    18181 ctgcgttcgc caaaaaagag atagccggaa atcatgaaaa atagcggcac gctgacacga
    18241 gaggcagaat tcagaacatt ggcgatatcc caggtgacgg ggctaacact atgagcattg
    18301 gtcacatacc aggtagtggt gtgaatcatc accaccatta aacacgctat ccctcgcagg
    18361 ttatcaatcc agtaaatttt gggctgcatc tgtgtctctg tatctggtta aaaaaagtct
    18421 gaccgataaa tcattcagtt ggcgcactgg aataatctga gttttatcac tacagcttat
    18481 agaggcttaa ggaaattcgt aagatatcag ccactatacc gatataaata ataagactca
    18541 cctgcaaacc agacggtaat ttaatgatga tgaacgcttt ctttccggca atggcgctta
    18601 tggtgctagt gggttgttct acaccgtcac ccgtgcagaa agcacaacgg gtaaaggttg
    18661 atcctctgcg ttcgttgaat atggaagcgt tatgcaagga tcaggcggca aaacgttata
    18721 acaccggcga gcaaaaaatc gacgtcaccg ccttcgaaca gttccaggga agctatgaaa
    18781 tgcgcggtta taccttccgt aaagagcagt ttgtctgttc ttttgacgca gatggccatt
    18841 ttttgcatct ttccatgcgt taagccctgc tttttcccgt ttcgtactgt atatcttcca
    18901 tccagcgggt atactgatcc cttcctttaa atccacacgt atccagcacg aaataatatg
    18961 caaaagtttg ataccaggac cttccagggc ttgatcctga ccttacagga ttactgggct
    19021 cgccagggct gcaccattgt tcaaccattg gacatggaag tcggcgcggg aacctctcac
    19081 ccaatgacct gtctgcgcgc gctggggcca gaaccgatgg cggctgctta tgttcagcct
    19141 tctcgtcgcc cgaccgatgg tcgctacggc gaaaacccca accgtttaca gcactactat
    19201 cagttccagg tggtcattaa gccatcgccg gacaatattc aggagctgta cctcggttct
    19261 ctgaaagagc tgggcatgga cccgactatt cacgacatcc gtttcgtgga agataactgg
    19321 gaaaacccga ctctgggtgc ctggggactg ggctgggaag tgtggctaaa cggcatggaa
    19381 gtgacgcagt tcacttactt ccagcaggtt ggtggtctgg agtgtaaacc ggttaccggc
    19441 gagatcacct acggtctgga acgtctggcc atgtacattc agggcgtaga cagcgtttac
    19501 gacctggtct ggagcgacgg cccgctgggt aaaaccacct acggcgacgt gttccatcag
    19561 aacgaagtgg agcagtccac ttacaacttc gaatacgcgg atgtggactt cctgttcacc
    19621 tgctttgagc agtacgagaa agaagcgcag cagctgctgg cgctggaaaa tccgctgccg
    19681 ctgccagcct acgagcgtat tctgaaagcc gcccacagct tcaacctgct ggatgcgcgt
    19741 aaagccatct ccgtcaccga gcgtcagcgc tacattctgc gcattcgcac cctgaccaaa
    19801 gcagtggcag aagcatacta cgcttcccgt gaagccctcg gcttcccgat gtgcaacaaa
    19861 gataagtaag aggcggctat gtctgagaaa acttttctgg tggaaatcgg cactgaagag
    19921 ctgccaccaa aagcactgcg cagcctggct gagtcctttg ctgcgaattt tactgcggag
    19981 ctggataacg ctggcctcgc acacggcacc gttcaatggt ttgctgctcc gcgtcgtctg
    20041 gcgctgaaag tagctaacct ggcggaagcg caaccggatc gtgaaatcga aaaacgcggc
    20101 ccggcgattg cccaggcgtt cgacgctgaa ggcaaaccga gcaaagcggc agaaggttgg
    20161 gcgcgtggtt gcggtattac cgttgaccag gctgagcgtc tgactaccga taaaggcgaa
    20221 tggctgctgt atcgcgccca tgtgaagggc gaaagcaccg aagcactgct gccgaatatg
    20281 gttgcgactt ctctggcgaa actgccgatc ccgaaactga tgcgttgggg cgcaagcgac
    20341 gtgcacttcg tgcgtccggt gcacaccgtg accctgctgc tgggcgacaa agtcattccg
    20401 gcaaccattc tgggcattca gtccgatcgc gtgattcgcg gccaccgctt tatgggcgaa
    20461 ccggaattca ccatcgacaa cgccgatcag tatccggaaa ttctgcgtga gcgcgggaaa
    20521 gtcatcgccg attacgaaga acgtaaagcg aagattaaag ccgatgccga agaagcagcg
    20581 cgtaagattg gcggtaacgc tgacttaagc gaaagcctgc tggaagaagt ggcttcgctg
    20641 gtggagtggc cggtcgttct gaccgcaaaa ttcgaagaga aattcctcgc ggtgccggct
    20701 gaagcgctgg tttacaccat gaaaggtgac cagaaatact tcccggtgta tgcgaacgac
    20761 ggcaaactgc tgccgaactt tatcttcgtt gccaacatcg aatcgaaaga tccgcagcag
    20821 attatctccg gtaacgagaa agtcgttcgt ccgcgtctgg cggatgccga gttcttcttc
    20881 aacaccgacc gtaaaaaacg tctggaagat aacctgccgc gcctgcaaac cgtgctgttc
    20941 cagcaacagc tgggtacgct gcgtgacaaa actgaccgca tccaggcgct ggctggctgg
    21001 attgctgaac agattggcgc tgacgttaac cacgcaaccc gtgcgggcct gctgtctaag
    21061 tgtgacctga tgaccaacat ggtcttcgag ttcaccgaca cccagggcgt tatggggatg
    21121 cactatgcgc gtcacgatgg cgaagcggaa gatgtcgcgg tggcgctgaa tgagcagtat
    21181 cagccgcgct ttgccggtga tgacctgccg tctaacccgg tagcctgtgc gctggcgatt
    21241 gctgacaaga tggatactct ggcgggtatc ttcggtatcg gccagcatcc gaaaggcgac
    21301 aaagacccgt ttgcgctgcg tcgtgccgca cttggcgtgc tgcgaattat cgttgagaag
    21361 aacctcaacc ttgatctgca aacgctgacc gaagaagcag tgcgtctgta tggcgataag
    21421 ctgactaacg ccaacgtggt tgatgatgtt atcgacttta tgctcggtcg cttccgcgcc
    21481 tggtatcagg acgaaggtta caccgttgac accatccagg cggtactggc gcgtcgtccg
    21541 actcgtccgg ctgatttcga tgcccgcatg aaagcggtat cgcacttccg tacccttgaa
    21601 gcagctgctg cactggcggc ggcgaacaag cgtgtctcca acattttggc gaaatctgac
    21661 gaagtgctga gcgaccgcgt gaatgcctct accctgaaag agccggaaga aattaaactg
    21721 gcgatgcagg ttgtggtgct acgtgacaag ctggagccgt actttgcgga aggtcgttac
    21781 caggatgcgc tggtcgaact ggcagagctg cgtgagccgg ttgatgtttt cttcgataaa
    21841 gtgatggtca tggttgatga caaagaattg cgtctcaacc gtctgaccat gctggagaaa
    21901 ctgcgcgaac tgttcctgcg cgttgcggat atttcgctgt tgcagtaata acgccgttat
    21961 taaaaagcct gccatctggc aggctttttt tattatcgct aaataataca gcaaccttta
    22021 ataatcttct gctgaataaa gattatctca tatattaatt ttatgaggtt tttttaggat
    22081 tatatcaagg agaagaaaca aacttattaa gctagaatag ccacgggtgc ttgagactgt
    22141 ttgtctcagg tattcaccga aaggcagaca gagaaaagcc ccacctgact ataaatcaaa
    22201 gtgaggctac cctatgcctg aacaccataa ggttagcctc ttactcgttg gaaatcaaca
    22261 cagggggctg ggaatgccgc agaaatatag attactttct ttaatagtga tttgtttcac
    22321 gcttttattt ttcacctgga tgataagaga ttcactgtgt gaattgcata ttaaacaggg
    22381 gagttatgag ctggcggcat ttttagcctg caatttaaaa gagtaagagt cttcggcggg
    22441 aagttattcc cgcctttctt acggcgttgc gcattctcat tgcacccaaa tttatttttc
    22501 acaaaaataa tactagattt tattacgcga tcgattattt atttcctgaa aaacaaataa
    22561 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
    22621 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
    22681 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacacg
    22741 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
    22801 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
    22861 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
    22921 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
    22981 accgttgatg tgcattaagc cacgcattgg cgggtgatgc aacaattatt tttcatattt
    23041 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
    23101 aaaagtctat tccatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
    23161 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
    23221 tgatacgccc aaaacccggg cgaaatcatc gatttttaac cctgtccctt ttcgtaactg
    23281 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
    23341 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
    23401 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
    23461 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
    23521 agtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttac
    23581 cactcctggt ttgtaaagat ttcgaaaatc taaccttctg cgaaggcgta aatctctggc
    23641 tacaaaaaaa gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
    23701 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
    23761 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
    23821 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
    23881 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
    23941 tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
    24001 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
    24061 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
    24121 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaacggttc
    24181 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
    24241 tgcgctaaaa cagaccgatg atttgaaacc ggtctttgat caggcattca ccaaagtggt
    24301 aacaacgcca gctgatgcat tgcaaccgtt gattccggct gcacaaactt tcacgcaaca
    24361 actggtgatg gttggagact acattgctca acaaggtact caggtgagct ttgtagcaaa
    24421 tggtatccag ttcccgactt cgcagcaggc aagtgaatat aacaaactga ttgcgccgtt
    24481 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
    24541 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcggc ttagtccgcg
    24601 acgtgcggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
    24661 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
    24721 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
    24781 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg cagcaatcag tgcattttca
    24841 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
    24901 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
    24961 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
    25021 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcgcacgt
    25081 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
    25141 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
    25201 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
    25261 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
    25321 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
    25381 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttcggcaaaa
    25441 attgctgcat tttgttcgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
    25501 tgctcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
    25561 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
    25621 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
    25681 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
    25741 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
    25801 caacgcgctg gcaacggaat ccgcacgttg ctgggagaga cgcatgttca ggtcgtgacc
    25861 acccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
    25921 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc agggtcgcgc tgctgctgtc
    25981 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
    26041 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
    26101 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
    26161 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
    26221 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
    26281 cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg ttaatcctta
    26341 ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc gtgaacatga
    26401 agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc aatacatcta
    26461 acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt ctgactgccg
    26521 cccactgggg gcccatgctg gttgaaaccg acggcaaaac cgtgtttagc tcgcgtggcg
    26581 cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag gttcacagca
    26641 atacgcgggt acgatttcca atggtgcgca aaggctttct cgcgtcaccg gaaaacccgc
    26701 aaggcattcg tgggcaggat gaatttgttc gcgtgagttg ggatgaggcg ctggatctta
    26761 ttcaccaaca acataaacgc attcgtgagg cttatggtcc ggcatcgatt tttgctggtt
    26821 cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta caacgctata
    26881 tggcgctggc aggcggttat atcgggcatc tgggggatta ttcgaccggt gcggcacagg
    26941 cgatcatgcc gtatgtcgtg ggtggcagcg aagtttatca acagcaaacc agttggccgc
    27001 tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc taacccactc aatacgctga
    27061 aaattgcgtg gaatgcatct gatgagcagg ggctttctta cttttctgca ctgcgtgaca
    27121 gtgggaaaaa gctgatctgc attgatccaa tgcgatcgga aaccgtcgat ttctttggcg
    27181 ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt tgcgctgatg ctggggatcg
    27241 cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcgt tgcaccacag
    27301 gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggaatagca aaaaccgccg
    27361 aatgggcagc agagatttgt ggtgttggcg cagcgaaaat ccgcgagctg gcggctattt
    27421 tccaccaaaa taccaccatg ctgatggcag gctggggaat gcagcgccaa cagtttggtg
    27481 agcaaaaaca ctggatgatc gtcacgctgg cagcaatgtt ggggcaaatc ggcacacccg
    27541 gcggcggttt tggtctttct taccattttg ccaatggtgg taaccccacg cggcgttctg
    27601 cggtgctctc ttccatgcag ggcagcttgc cgtgtggctg cgatgcggtg gataaaatcc
    27661 ctgttgcccg cattgttgaa gcactggaaa accctggtgg cgcatatcaa cacaacggta
    27721 tgaaccgaca tttcccggat attcgtttta tctggtgggc gggcggtgcc aactttactc
    27781 atcatcagga taccaatcgt ctgatccgtg cctggcaaaa accggagctg gtggtgatct
    27841 ctgaatgctt ctggacggcg gcggcgaaac acgcggatat cgttctgcct gcgactacct
    27901 cttttgagcg taatgatctc accatgaccg gtgattacag taatcagcat ctggtgccga
    27961 tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt tttgccgagt
    28021 taagtgaacg ctgggagaag ggcggttatg cacgttttac ggaaggaaaa agtgagctgc
    28081 aatggctgga aacgttttat aacgttgccc ggcagcgcgg ggcaagccag caggttgaat
    28141 tgccgccatt tgctgagttc tggcaagcca accagttaat tgagatgccg gaaaacccgg
    28201 acagcgagcg gtttattcgc ttcgctgatt tttgccgcga tccgctggcg catccgttaa
    28261 aaaccgccag cggcaagatt gaaatcttct cacagcgtat tgccgattac ggttacccgg
    28321 attgccctgg gcatccaatg tggctggagc cggacgaatg gcagggcaat gccgaaccag
    28381 aacagttgca ggtactttct gcccatccgg cgcaccgcct gcacagccag ctgaattaca
    28441 gttctctgcg cgaattgtac gcggtagcaa accgtgagcc tgtcaccatt catcctgacg
    28501 atgctcaggc gcgcggcatt caagatggcg atactgttcg gctgtggaac gcacgcgggc
    28561 aaattcttgc cggagcggtc attagcgagg gaattaaacc tggcgtgatt tgtattcacg
    28621 aaggggcatg gccggatctg gatttaaccg ctgacggtat ttgtaaaaac ggcgcagtca
    28681 acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg ggtaatacgg
    28741 cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg tttgaaccac
    28801 cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc aatcgacaat
    28861 gtgaaaaccc tgcgcacggt aaaaatctat cgccggttga tttttttgat aaacctccag
    28921 catcagatgg ggatagcgct gctgcacata ctgcatcagc gccttaccaa taccgcgtct
    28981 gacggccttc ggtgcgacaa acattgctgc cagaaatcgg ccttccataa tgctgacaaa
    29041 accgagaagc ttaccgtcct cttcccagac ccagttttgc gcgttggcaa gataggcatc
    29101 ccgcaccagc ggaatgcagt cacgccagta attcgctttt ataaagggat gcccccaggt
    29161 tgtactttcc agccacagtt cgaggatcgc ggggagttct gaacgttgcg cttcccgaat
    29221 catggtttat ttcccggata gcaacagcag ccaaccacat gatcattcac cagcccacat
    29281 gcctgcataa aggagtaaca gattgtggtg ccgacaaact taaaaccacg ctttttcagg
    29341 gctttagata gagcgtcgga ggcggacgta gatgtgggaa tttcgctcaa cgttgtggct
    29401 tgtgtcacct gtggctgatg atttacaaac gaccagacaa agtcggcaaa cggttcgccg
    29461 ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat tttccctcga
    29521 tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg cattgctgcg
    29581 accttcaccg gatcgaactg atgaaagcag gcgcgatagt tttcgcgttt tttgaggacg
    29641 gtgatccacg ataacccagc ctgctgccct tcaaggcaga tcatttcgaa cagtttttta
    29701 ctgtcagttt caggcacgcc ccattcatta tcatggtagg caatataaag cgggtcctga
    29761 ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt aaaaatttca
    29821 gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct actatgcccg
    29881 atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg agtcgtgtta
    29941 atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgctggcgag cgtggtaatc
    30001 agtgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt tgatacgcaa
    30061 cccggacatt ccacagagcg ttacacctgg gatagtgatc atcaacctga ttacaacgat
    30121 attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt caatctggcg
    30181 gaagaaactc ctgtggatgt gaccagcagt atgagtatgg gctggaattt tcctttatat
    30241 gaacaggtta caaccggccc ggtcgcggca ttacattacg atggcacaac cacctcgatg
    30301 tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca tgccagcgtg
    30361 agtagcttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg ggcgcaaatc
    30421 agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct gagccggatg
    30481 acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga tatgttgctc
    30541 aatcaaaata ttgccgccta tgccgcgcta actcaggcag aaaataccac taataatagc
    30601 gactatctgt atacgatggg ggttagcgcc agattttaac gtaacagtca caattgaaac
    30661 cattgaataa caacagttgt ggcgatagtg ggtgctaact taccaaataa taaatttggt
    30721 gaataattgt cgcgtcattc attcctgaac taaggcattt cattccgttc tgatggcatt
    30781 tcatgccgtt tttccccagg cataaagtgc acttcgttat ggttgtcggc agagattttt
    30841 cctttttatt actgcaggaa tactgccatg acaccttcaa attatcagcg tacccgctgg
    30901 ctgacactca tcggtacaat cattacccag tttgcgctgg ggtcggttta tacctggagc
    30961 ctgtttaatg gcgcgctttc cgccaagctg gatgcgccgg taagccaggt cgctttctct
    31021 ttcggcttgt taagtctggg gctggcaatt tcatcttctg ttgcgggcaa attacaggaa
    31081 cgttttggcg ttaaacgcgt caccatggct tccggcactt tgctgggatt aggcttcttc
    31141 ctgaccgcgc attctgacaa cctgatgatg ctgtggttaa gcgccggtgt gctggtcggt
    31201 ctggcggatg gcgcgggtta cctgctgacg ctctctaact gcgtgaagtg gttcccggag
    31261 cgtaaaggtc tgatatccgc gttcgctatc ggttcttatg gtctgggtag cctgggtttc
    31321 aaatttatcg acacgcagct gctggaaacg gtgggtctgg aaaaaacctt tgtgatttgg
    31381 ggagcgattg cgctggtgat gattgttttc ggcgcaacgt taatgaaaga cgcaccaaaa
    31441 caggaagtga aaaccagcaa tggtgtggtg gagaaagatt acacgctggc agagtcgatg
    31501 cgtaaaccgc agtactggat gttagcggta atgttcctga ctgcctgcat gagcggcctg
    31561 tacgtgattg gggtagcgaa agatatcgcc caaagtctgg cacaccttga tgtggtttcc
    31621 gcagctaatg cagtcactgt tatttccatc gccaaccttt caggtcgtct ggttctgggg
    31681 atactgtctg acaaaatcgc ccgtatccgt gttatcacca ttggtcaggt gatttcgcta
    31741 gtgggtatgg cggccctgct gtttgcacca ctgaatgcag tgacgttctt tgcagcgatt
    31801 gcctgcgtgg catttaactt tggcggcact attaccgtct ttccgtcact ggtcagtgag
    31861 ttcttcggcc tcaataacct ggcgaaaaac tacggtgtga tttacctcgg tttcggtatc
    31921 ggtagcattt ttggttcgat tatcgcctca ctgtttggcg gtttctatgt gaccttctac
    31981 gtgattttcg ccctgctgat tctgtcattg gcgctttcta cgacgattcg tcagccagag
    32041 cagaaaatgt tgcgtgaggc gcatatttag aaatatgttg ctactgaaat aaatacgagt
    32101 agggagactt caaagtctcc cttttttatg gacgcatatt aaaagaacaa taatattcat
    32161 acca
//
LOCUS       DAZGSP010000055        32045 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZGSP010000055 DAZGSP010000000
VERSION     DAZGSP010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.055"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(162..290)
                     /gene="rhuM"
                     /locus_tag="ACHK74_002783"
                     /pseudo
     CDS             complement(162..290)
                     /gene="rhuM"
                     /locus_tag="ACHK74_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            complement(369..463)
                     /locus_tag="ACHK74_002784"
     tRNA            complement(369..463)
                     /locus_tag="ACHK74_002784"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(427..429),aa:Sec,seq:tca)
     gene            756..2138
                     /gene="yicJ"
                     /locus_tag="ACHK74_002785"
     CDS             756..2138
                     /gene="yicJ"
                     /locus_tag="ACHK74_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFP0221572.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            2148..4466
                     /gene="yicI"
                     /locus_tag="ACHK74_002786"
     CDS             2148..4466
                     /gene="yicI"
                     /locus_tag="ACHK74_002786"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFP0221573.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPATDGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(4519..6228)
                     /gene="yicH"
                     /locus_tag="ACHK74_002787"
     CDS             complement(4519..6228)
                     /gene="yicH"
                     /locus_tag="ACHK74_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP0221574.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPKL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(6349..7740)
                     /gene="xanP"
                     /locus_tag="ACHK74_002788"
     CDS             complement(6349..7740)
                     /gene="xanP"
                     /locus_tag="ACHK74_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFP0221575.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            8020..9225
                     /gene="gltS"
                     /locus_tag="ACHK74_002789"
     CDS             8020..9225
                     /gene="gltS"
                     /locus_tag="ACHK74_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFP0221576.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            9228..10094
                     /locus_tag="ACHK74_002790"
     CDS             9228..10094
                     /locus_tag="ACHK74_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221577.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(10079..12160)
                     /gene="recG"
                     /locus_tag="ACHK74_002791"
     CDS             complement(10079..12160)
                     /gene="recG"
                     /locus_tag="ACHK74_002791"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFP0221578.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKQTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(12166..12855)
                     /gene="trmH"
                     /locus_tag="ACHK74_002792"
     CDS             complement(12166..12855)
                     /gene="trmH"
                     /locus_tag="ACHK74_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFP0221579.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(12862..14970)
                     /gene="spoT"
                     /locus_tag="ACHK74_002793"
     CDS             complement(12862..14970)
                     /gene="spoT"
                     /locus_tag="ACHK74_002793"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFP0221580.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(14989..15264)
                     /gene="rpoZ"
                     /locus_tag="ACHK74_002794"
     CDS             complement(14989..15264)
                     /gene="rpoZ"
                     /locus_tag="ACHK74_002794"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP0221581.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(15319..15942)
                     /gene="gmk"
                     /locus_tag="ACHK74_002795"
     CDS             complement(15319..15942)
                     /gene="gmk"
                     /locus_tag="ACHK74_002795"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP0221582.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            16200..17882
                     /gene="ligB"
                     /locus_tag="ACHK74_002796"
     CDS             16200..17882
                     /gene="ligB"
                     /locus_tag="ACHK74_002796"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFP0221583.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(17879..18496)
                     /gene="yicG"
                     /locus_tag="ACHK74_002797"
     CDS             complement(17879..18496)
                     /gene="yicG"
                     /locus_tag="ACHK74_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFP0221584.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(18787..19389)
                     /gene="dinD"
                     /locus_tag="ACHK74_002798"
     CDS             complement(18787..19389)
                     /gene="dinD"
                     /locus_tag="ACHK74_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFP0221585.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQANTTHFD
                     VGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(19610..20473)
                     /gene="yicC"
                     /locus_tag="ACHK74_002799"
     CDS             complement(19610..20473)
                     /gene="yicC"
                     /locus_tag="ACHK74_002799"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFP0221586.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            20600..21316
                     /gene="rph"
                     /locus_tag="ACHK74_002800"
     CDS             20600..21316
                     /gene="rph"
                     /locus_tag="ACHK74_002800"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFP0221587.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            21382..22023
                     /gene="pyrE"
                     /locus_tag="ACHK74_002801"
     CDS             21382..22023
                     /gene="pyrE"
                     /locus_tag="ACHK74_002801"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFP0221588.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(22060..22656)
                     /gene="slmA"
                     /locus_tag="ACHK74_002802"
     CDS             complement(22060..22656)
                     /gene="slmA"
                     /locus_tag="ACHK74_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFP0221589.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(22763..23221)
                     /gene="dut"
                     /locus_tag="ACHK74_002803"
     CDS             complement(22763..23221)
                     /gene="dut"
                     /locus_tag="ACHK74_002803"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFP0221590.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(23199..24419)
                     /gene="coaBC"
                     /locus_tag="ACHK74_002804"
     CDS             complement(23199..24419)
                     /gene="coaBC"
                     /locus_tag="ACHK74_002804"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFP0221591.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARIAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGMLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            24591..25259
                     /gene="radC"
                     /locus_tag="ACHK74_002805"
     CDS             24591..25259
                     /gene="radC"
                     /locus_tag="ACHK74_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP0221592.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            25476..25712
                     /gene="rpmB"
                     /locus_tag="ACHK74_002806"
     CDS             25476..25712
                     /gene="rpmB"
                     /locus_tag="ACHK74_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFP0221593.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            25733..25900
                     /gene="rpmG"
                     /locus_tag="ACHK74_002807"
     CDS             25733..25900
                     /gene="rpmG"
                     /locus_tag="ACHK74_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP0221594.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            25998..26807
                     /gene="mutM"
                     /locus_tag="ACHK74_002808"
     CDS             25998..26807
                     /gene="mutM"
                     /locus_tag="ACHK74_002808"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFP0221595.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVELVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLTHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(26846..27325)
                     /gene="coaD"
                     /locus_tag="ACHK74_002809"
     CDS             complement(26846..27325)
                     /gene="coaD"
                     /locus_tag="ACHK74_002809"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFP0221596.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRAAQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(27333..28610)
                     /gene="waaA"
                     /locus_tag="ACHK74_002810"
     CDS             complement(27333..28610)
                     /gene="waaA"
                     /locus_tag="ACHK74_002810"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFP0221597.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            29023..30081
                     /gene="rfaQ"
                     /locus_tag="ACHK74_002811"
     CDS             29023..30081
                     /gene="rfaQ"
                     /locus_tag="ACHK74_002811"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFP0221598.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            30078..31202
                     /gene="waaG"
                     /locus_tag="ACHK74_002812"
     CDS             30078..31202
                     /gene="waaG"
                     /locus_tag="ACHK74_002812"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP0221599.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            31195..31992
                     /gene="rfaP"
                     /locus_tag="ACHK74_002813"
     CDS             31195..31992
                     /gene="rfaP"
                     /locus_tag="ACHK74_002813"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFP0221600.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYSADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         7573 a         7936 c         8618 g         7918 t
ORIGIN      
        1 tgatggcagt ctgattattg gtgaagttta tagtcagttt ttttcgccag cggttaaaga
       61 aatagaatag tcgtataaaa aagggtgttc tggggcgagt aaaacaacat cttcacctta
      121 atggcgatac agttggctga gagtcagcag ggggggcaaa tcacctgcaa ataaacacgg
      181 cgcatactgg cgtgaatatc gcggatgcgt tcgaggacag aagaaccgac aggcggattc
      241 ttcagccgct catcgtccat cacaaaacct tagaccaggt attcctgaag ccgattgaat
      301 attgagcagt agaaaatatc tggattgaca gtattacaag gggttggaga acgtcaggag
      361 aggcattttg gcggaagatc acaggagtcg aacctgcccg ggaccgctgg cggccccaac
      421 tggatttgaa gtccagccgc ctcaccggag acgacgatct tccgcgcctc gattgctaca
      481 tggaggcggg gcgcattata gctacttcct tgagtttcta catcccccag atcagttttt
      541 tccgcctaat acacgcagcg tattatcacg aaagcattat agttcatata aaacctatat
      601 tttacaatga tatatgagcg aaattaacgc taccacgatc acataaaaca agaatcataa
      661 attaataacc agatatcgga atattcgctc tcacagggat ggtttacaaa atgcgttatc
      721 ggtctcagca cccctatagc atcaaggaaa agcagatgaa gagtgaagtg ttgtccgtta
      781 aagagaaaat tggttatggc atgggagacg ccgccagcca cattattttc gataacgtaa
      841 tgttatatat gatgttcttt tataccgata tttttggcat tcctgccggt tttgtcggaa
      901 ccatgttttt ggtcgctcgt gcactggatg cgatttccga tccttgcatg gggttgttgg
      961 ccgatcgaac gcgctctcgc tggggtaaat ttcgtccgtg ggtactgttt ggcgcactgc
     1021 cattcgggat cgtctgtgta ctggcctata gcacgccaga tctcagtatg aacggcaaaa
     1081 tgatctatgc agcaattact tacaccctac ttaccttact ttataccgtc gtcaatatcc
     1141 cttactgcgc attgggtggt gtaatcacca atgacccgac tcagcgtatc tcgctgcaat
     1201 cctggcgttt tgtgctggcg accgcgggag gcatgctttc tactgttctg atgatgccac
     1261 tggttaattt aattggcggt gataataaac cactcggttt ccagggcggt atcgcggtcc
     1321 tttccgtggt ggcattcatg atgctggcat tctgtttctt caccactaaa gaacgcgttg
     1381 aagcaccacc tacaacaacg tctatgcggg aagatttacg tgatatctgg caaaacgacc
     1441 agtggcggat tgtcggttta ctaaccattt tcaatatcct ggcggtgtgc gtacgcggtg
     1501 gggcgatgat gtattacgtc acatggattt tgggcacgcc ggaagtgttt gtcgcttttc
     1561 tcaccactta ttgcgtgggt aacctgattg gttccgcact ggcaaaacct ctgaccgact
     1621 ggaaatgtaa agtcactatc ttctggtgga cgaacgccct gctggcagtg attagcctcg
     1681 cgatgttctt tgttcccatg caggccagca tcactatgtt tgtcttcatc ttcgtgattg
     1741 gtgtgttgca tcaactggtg acacctatcc agtgggtaat gatgtccgat accgtcgact
     1801 acggcgagtg gtgcaatggt aaacgcctga ccgggatcag ttttgctggc acgctgtttg
     1861 tgctcaaact ggggttggcc ttcggcggcg ctcttatcgg ctggatgctg gcttatggcg
     1921 gatatgatgc ggcagaaaaa gcgcagaaca gcgccacgat tagcatcatt attgcgctat
     1981 tcacgattgt tccggcgatc tgttatttgc tgagcgcgat tatcgctaaa cgctactact
     2041 cactcacgac gcacaatctg aaaaccgtta tggaacagct ggctcagggt aaacgccgtt
     2101 gccagcaaca attcacctct caagaagtgc agaactaagg aacggcaatg aaaattagcg
     2161 atggaaactg gttgattcaa cctggcctca atttgattca cccgcttcag gtgttcgagg
     2221 ttgaacagca ggataatgaa atggtggtct atgctgcccc ccgtgatgtg cgtgaacgta
     2281 cctggcagct tgatacgcct ttatttacgt tgcgcttttt ctccccacag gaaggtattg
     2341 tcggtgtgcg gattgagcat tttcaggggg cgctgaataa cggtcctcat tatccgctca
     2401 atattttgca ggacgtgaag gtcacaatcg aaaacacaga acgttatgct gagtttaaaa
     2461 gtggcaactt aagcgcgcgt gtcagcaaag gtgagttctg gtcactggat tttctgcgca
     2521 acggcgaacg tattaccggt agtcaggtga aaaataatgg ctacgtgcag gacacgaata
     2581 atcaacgcaa ttatatgttt gagcggcttg atcttggcgt tggcgaaaca gtttacggtc
     2641 tgggagagcg ctttactgcc ttggtgcgca atggccagac ggtagagacc tggaaccggg
     2701 acggcggcac aagtactgaa caggcgtata aaaatatccc gttctacatg actaaccgtg
     2761 gttatggggt actggtcaat catccccagt gtgtctcttt tgaagtggga tcggagaaag
     2821 tctccaaagt gcagttcagc gttgagagtg aatatctcga atactttgtt atcgacggcc
     2881 cgacgccgaa agcggtactt gatcgttata cccgctttac tggtcgtccg gcgctgccgc
     2941 ccgcgtggtc cttcggcctg tggctaacca cttcatttac caccaactac gacgaagcga
     3001 cggtaaacag ctttatcgat ggtatggcgg aacgcaatct gccgctgcat gttttccact
     3061 ttgactgttt ctggatgaaa gccttccagt ggtgcgattt tgagtgggac ccgctgactt
     3121 tccctgaccc ggaagggatg atccgccgcc tgaaagcgaa aggactgaaa atctgcgtct
     3181 ggattaaccc ctatatcggt caaaaatccc ccgtctttaa agagttacaa gagaaaggct
     3241 atttactcaa acgcccggac ggttcgctat ggcagtggga taaatggcag ccaggtctgg
     3301 cgatttatga ctttaccaat ccggatgcct gcaaatggta cgccgacaaa ctgaaaggtc
     3361 tggtcgcgat gggcgttgat tgctttaaga ccgactttgg cgaacgtatc ccaactgatg
     3421 ttcagtggtt tgacggttcc gatccgcaga aaatgcataa ccattatgcg tacatctaca
     3481 acgaactggt gtggaacgtg ctcaaggaca ccgttggtga ggaagaagct gtcttgtttg
     3541 cccgctcggc ctccgtcggt gcgcagaaat tcccggtaca ctggggtggc gattgttacg
     3601 ctaactacga atcaatggcg gaaagcctgc gcggtggttt gtctattggc ctttcaggtt
     3661 ttggcttctg gagccacgat atcggcggct ttgaaaatac cgctccggcg cacgtttaca
     3721 aacgctggtg cgcgtttggt ttgctctcca gccatagccg tttacacggt agcaaatctt
     3781 atcgtgtgcc gtgggcctac gatgatgagt cctgtgatgt ggtgcgcttc ttcacgcaac
     3841 tgaaatgccg catgatgccg tatctgtatc gtgaagctgc gcgtgcgaac gcgcggggta
     3901 cgccgatgat gcgggccatg atgatggaat tcccggacga tccggcttgt gattaccttg
     3961 accgtcaata catgttaggc gacaacgtga tggttgcgcc ggtgttcact gaagcgggcg
     4021 atgtgcagtt ctacctgccg gaaggtcgct ggacacacct gtggcacaac gatgaactcg
     4081 acggtagtcg ctggcataaa cagcagcacg gcttcctgag tctgcccgtt tatgtgcgtg
     4141 ataacactct actggcgctg ggcaacaacg atcaacgtcc cgattacgtg tggcacgaag
     4201 gcacggcatt ccacctcttt aatctgcaag acgggcatga agccgtctgt gaagtgcccg
     4261 ctactgacgg atcggtgatc tttactttaa aagcagcacg tactggcaac acgattactg
     4321 tgactggtgc gggcgaggcg aagaactgga cgctgtgcct gcgcaatgtt gtgaaagtaa
     4381 atggtctgca agacggttcg caggctgaaa gtgagcaggg gctggtggtg aagcctcaag
     4441 ggaatgcgct gacaattacg ttgtaatcta ttgatggaat gccgggtacg tctgagtcgt
     4501 acccggcgag attgatgttt accgcaccgg ctccgtcgat gtaacttcac cgctggaaac
     4561 gatgccagca ttcatggttt gagtcacttg ctgctttgcg gcgttcacgg catgcaattg
     4621 cccgctaacc gtaggtttca acgggacatt ggcctgaata tcgccactgg cggtaagctg
     4681 aatattgccg tcgccagtca acggtaattt aggccatccc cactgttgca aaatattcac
     4741 cggcacaccg cgtccattca ggctgatatg tgtctgacgt tgtggcgttt gtgaaacact
     4801 ggcggtggct tccagaatgc ctttttcagt aaatgcactc agttcgctga tattcaccgt
     4861 gctgctgttg gcggtcagcg ccagcgacgg gcgacgaaca tcaacacgat tgaatgtcgc
     4921 ggcggcggca ttcagattcg ccgagccact ccagacgccc catttatgat cggtaaccag
     4981 cgtcaggttg gcaccgtaac catcgagcgc ggtgagctgc cacgggaagt cagggtcgat
     5041 atcaatgatc agattgcggc tggcgctaaa tctcttcagt tgcaggctgt ttaaccaacc
     5101 gggtgtcgtt tccatccaca actgttgcca gtttttcggc aaggtatatt ccagcccggc
     5161 aattgccgca tcatcaagga tcaacgtttt cccgtcacgc agccagttcc ctgacgttct
     5221 gaccataccc ccttcccagc ggctggtgaa ctggcgcagc gctacgccct gcggggaaaa
     5281 ttccgcgttt ataatcgggt caaataaatg cagcgaacca taaatgaact cgctagcgtt
     5341 catcgacagt ttgccttctt gtgtctgcca gtcatcttta ctgaaggtca tgttgcgcaa
     5401 gctgagatcg aggtcggtca ccgcccagtc cggaccttgc aaacgagcat cgatcacttc
     5461 caggcgacca atctgcaacg acgggacaga gcgtaatggc gcaaagaagt cggttagcga
     5521 tttttcgctt tgtagacgga tatccgccat gcgcagattt tccacttgcc agctgccgtc
     5581 ggcgttacgc tgcgcgtttc cggttaatgt cccgcgggcg atgtcggcac ccaggttagt
     5641 cagcgtaacg cgatcgttat caatactgcc ttcaatcagt acattggtgg caggaacatc
     5701 attgagcgaa agcgatccgg cactaaactg aatctgcgcc ttcgtaccca gcactttacc
     5761 ggcttctggt gaccacggaa ccacgccgcc atttacccgc tgcgcgctca gtttccattc
     5821 gctattcggg ctattaaacg ccatatcacg cagttgcaga cgatcggctt tgaacggtag
     5881 cggcgcggtc tggtcggtga gattcagcgt cccgttttcc agcaggatgg tatcgacatg
     5941 gcgtggttcg gtcagttgcc gactgcttag cgcaatgtcg acactttttg ccaccagggt
     6001 cgcgggctga ccatcacgac caaacgtgac gttctccagc acgatatgag atggcgcgga
     6061 aaaacggtga tccatcgccc cgaaggccag atgatagtcg ctattctcgg aaacccatgc
     6121 gctgatatgt tctgctcccc agcgggtttg cagaagaaaa tagaggccag cgatcaccac
     6181 taacagagcg atgagaatgt agagaagcag cttcccaata aatttcatgg tcttccatcc
     6241 cgtgaaatgc acataaggga gttatgcacg atttacgcgc aatcctcaag gcgggaatgg
     6301 tgaaagaggt gagactgcgg cggtaattat tttaccgccg ccagtgaatt actgtttttc
     6361 tggtgggaaa atcagattca gcacgatggc agtaataccg cccgcggcga tcccggagga
     6421 gagcaggttt ttcagccatt caggggcaaa ctgcaaaatc agcggctgct gagacacgcc
     6481 cagaccaacc gccagcgaca gcgcgataat cagaatcgcc cgacggttca gcggctcacg
     6541 agaaacgata cgcacaccgg aggcggcgat ggtgccaaac attacaagcg ttgcgccgcc
     6601 cagaaccggt tctggaatgt gttgtacaaa accgctcact gccgggaaca gacccagcac
     6661 gatcaacatc agcgcgacga caaaaccgac atagcggctg gcaacaccag tcaactggat
     6721 cactccgttg ttttgcccga agcaggagtt cgggaaggtg ttaaacaccg ccgaaacaaa
     6781 cgagttcagg ccgtttgcca gcacgccgcc tttcaggcgt ttcatgtaca gcggaccgga
     6841 cactggctgt tcggaaacgt cagaggtcgc cgtgatatcg ccaatggttt ccagcgaagt
     6901 gatcataaag accagcatca gcggcagcag cagactccat tcaatgccaa gaccgtaata
     6961 gagcggcgtt ggcaccataa tcagttcttg cgtcatcggt tcgttgcttt ctggcaacat
     7021 gcccataaac cacgccagcg catatccggc cgccatcgca atcaccagtg aggccacgcg
     7081 taagtaaggg ttacgttgac ggttaagcag gataattaag gctaagacca cgcctgccag
     7141 cagcagattt ttcggtgcgc cgaaggtgtt atcgctcatg gctgcgtaac cgccgccaat
     7201 ggacgttaac ccaacctgaa ttagcgacag gccgataatc atcaccacaa cgccagaaac
     7261 cagcggcgta ataatgcggc gcgccagatg cagaacgcgg gagatcacca tctcggtgca
     7321 acttgccagc atcaacgtgc cgaacaaagc cgccatcatg gtaggaacat cagcaccacc
     7381 ggttttcagc gcggtaccgc ccataatcag cggggcaaca aagttgaagc tggtgccctg
     7441 aatagacaac agcccggagc caaccggacc ccaggcctta atttgaataa tcgatgccac
     7501 accggaggca aacagcgaca tactaataat gtgttgcgtg tcttgtgccg gtaaacccag
     7561 cgcctggcag attaatagcg ctggcgtgat caccgcaacg aacatcgcca gcagatgctg
     7621 acaggcggca aacagggttt gaggaagcgg cggacgatct tcaagacggt aaatcagttc
     7681 gctgttttga gtctgcgcaa ccggttgcgc attttctgac tcgagggtgg aaacagacat
     7741 cgaaaacaat cccgtggtag caaagcgggc attttagctg accgaaagag aaaagcaaac
     7801 gtttgccaca tcctcatcta aatatttaaa tgagataaat gcactttttt atataacttt
     7861 tggtattttt ctgcttaaaa tccatgccat ttgttcatta acagctgaga atttgaccca
     7921 ggaaagcata actcgatacc cgtcatagtt catgaagttg ccactgcgcg atgaagtatg
     7981 acgagtatga aagagtgatg cggatacaaa ggagtaacta tgtttcatct cgatacttta
     8041 gcaacgcttg ttgccgcaac gctgacgttg ctgctcgggc gtaagttggt ccattccgtc
     8101 tcctttttga agaaatacac cataccggaa cctgttgcgg gtggtttgtt ggtggcgctg
     8161 gcgctactag tactgaaaaa aagcatgggc tgggaagtca actttgatat gtccctgcgc
     8221 gatccgttaa tgctggcttt cttcgccacc attggcctga acgccaacat tgccagtttg
     8281 cgtgccggtg ggcgtgtggt tggcatcttc ttgattgtgg ttgttggtct gttggtgatg
     8341 caaaatgcca ttggcattgg tatggctagc ctgttagggc ttgatccgct gatggggctg
     8401 ttggccggtt ctattacgct ttccggcggt cacggtacgg gcgctgcgtg gagtaaattg
     8461 ttcattgaac gttatggctt caccaatgcg acggaagtgg cgatggcctg tgcaacgttc
     8521 ggtctggtgc tgggcggctt gattggcggt ccggtggcgc gctatctggt gaaacactcc
     8581 accacgccga acggtattcc ggatgaccag gaagtcccga cggcgtttga aaagccggat
     8641 gtgggacgca tgatcacctc gctggtgctg attgaaacta tcgcgctgat tgctatctgc
     8701 ctgacggtgg ggaaaattgt tgcgcaactt ttggctggca ctgcttttga actgccgacc
     8761 ttcgtctgtg tactgtttgt tggcgtgatt ctgagcaacg gtctgtcaat gatgggcttt
     8821 taccgcgtct ttgagcgtgc ggtatccgtg ctgggtaacg taagcctgtc gttgttcctg
     8881 gcgatggcgc tgatggggct gaaactgtgg gagctggctt cgctggcgct gccgatgctg
     8941 gcgattctgg tggtacagac catcttcatg gcgttgtatg ccatcttcgt tacctggcgc
     9001 atgatgggca aaaactacga tgcggcagtg ctggctgcgg gtcactgtgg ttttggcctc
     9061 ggtgcaacgc caacggcaat cgccaacatg caggcgatca ctgaacgctt tggcccgtcg
     9121 cacatggcgt ttttggtggt gccgatggtc ggtgcgttct ttatcgatat cgtcaatgcg
     9181 ctggtgatta agctgtattt aatgttgccg atttttgccg gttaaaaatg aaaaagcgtc
     9241 ggaaggaacc ggaaacgcta cgtgaacact gtcgccatat ttttggtgat gagccgccag
     9301 tgctctgtgt atgggaaacg gaatttgatt acgcagatgc cgaactcaag gcgctggcgg
     9361 ctaaagagtg gcagcagatc agcgaatggg atttgagtgc ttactatgta ctcaatctcg
     9421 tttataacga accgatgcaa atagagttgt ttcgctatct gtttccgctc tgcctggcgc
     9481 aatggcatga aaccgtttta gctggcggtt atggtgatca cttcgaagaa tccttaatga
     9541 aggcattgtg tcggccctat ctctggcagg agatgatgaa tgcttcccaa cgccagcagg
     9601 tacggcaatt tttactggac accgcgttgc agcgtatgga taacgagcgt ggtttcaata
     9661 acgttctttg ctggctggcg gtgttcaata ctctgggtgg ggccgcgccg ttaattcgtt
     9721 ctctctggtc gcgctggtgg gcgctggata cgccaggaaa agcggtgtgt gctattcagt
     9781 atgccgcgca tctaatttat cccatagaag ccaatccgtt atggtcgcaa gaatggatag
     9841 gctgggggca tccattaggc cataaagatg gctggtcctc tgataatcgc gcttttcttc
     9901 gccaaatgtt aacacctgaa atgattgtgg ctggcgtaca ggcagcggct gaaattctgc
     9961 gtggtgaacc ggaaggcgca atggcggcgc gaattgcaca ggatgcgtat gaggcaatgg
    10021 atatcctcac tatccagatt gaagatttac tgcgtgactt gtcttgtgat gagtctggtc
    10081 atgcgttgga gtaacgttcc gtctccggca tccagcgttc tatcagggct tttgcttgtt
    10141 gtgggtaacg ttcgtgaata tgtcgcgcca ggcgctgaac ttccgggatc atcgcctgat
    10201 cgcgcagtaa atccgccact ttaaactcag cattacccgt ctgacgcgta cctaacagtt
    10261 cacccggtcc gcgaatctct aagtctttct gtgcaatcac aaaaccgtcg ttgctgtcgc
    10321 gcagcacttg cagccgaatt tgcgcggttt tggagagcgg cgttttgtag agcagcacgc
    10381 agtgagaagc caccgcacca cgacctacac gcccgcgaag ctggtgtaac tgcgccagac
    10441 ccagacgctc cgggttttcg ataatcatca gactggcgtt aggcacatca acgccgactt
    10501 caataacggt tgtggcaacc agcaggtgta gctcaccttg tttaaacgac gccatcaccg
    10561 cctgtttctc ggcaggtttc atccgcccgt gtaccaggcc aacgttcaac tctggtagcg
    10621 ccagtttcaa ctcttcccag gtagcttccg ccgcctgcgc ttccagcaat tccgactctt
    10681 caatcaacgt acaaacccag tatgcctgac gaccttcagt tatgcaggcg tggcgcacgc
    10741 ggtcaatgat gtcggtacgg cgggtatcag gaatagcgac cgtagtcact ggcgtgcggc
    10801 ctggcggcag ctcatctatc accgaggtat cgagatcggc atacgcagtc attgccagcg
    10861 tgcgggggat cggcgtggcg gtcatgatca actgatgcgg atggaagccc tgctgctggc
    10921 ctttctccca caatgccaga cgctgatgta cgccaaaacg atgctgttcg tcgataatca
    10981 ccagcgccag gccgttaaac tgcacctgtt cctggaagat ggcgtgtgta ccgacaatca
    11041 tctgcacctg accgctggcg atggcttcct gctgtgccag ccgtgcttta cctttctgct
    11101 taccggcgag ccagcccact tcgataccga gcggtgcaaa ccagttgcgg aagttattgg
    11161 cgtgctgctc ggcgagtaat tcggttggtg ccatcaatgc tacctgtttg ccgtgggcaa
    11221 tcgcacgcaa cgcggcgagg gcggcgacca gcgttttacc ggaacctaca tcgccctgca
    11281 ccagacgcat catcggcaca tccagcgcca tatcgcgctc gatctccgcc actacgcgtg
    11341 cctgtgcgcc cgtttgcttg aacggtaagg cggcgaggag tttatttttc agcgtgtcat
    11401 tggcgctcag cggctgggca tgaaaacgct gtgctccggc acgtaaggct aacatgctga
    11461 ggttgtgcgc cagcagttct tccagaatca gacgacgttg cgccggatgc tgcccggttt
    11521 ccagatcgct aagctgtagc gtcggtggcg ggcggtgcaa agtgcgcaac gcttccggta
    11581 gcgtcatcat tccttgtgac agttccggcg gcaggagttc ttcaatggcg caggtgtcga
    11641 gcagatccag cgcctggtcg gttaatttac gcagcgtggc ctgctttacg ccttccgttg
    11701 ttggataaac cggcgtgagc gtttcctgta attctggcgt gctgagatcg ccctgcacgc
    11761 ggtattccgg gtggatcatc tccgcaccat atttaccgcg ctttgcttcg ccataagcca
    11821 gtacacggcg gcccgtcgcc aggctatttt tcattgccgc gctgaaattg aaaaagcgca
    11881 tggtgaggat gccggaaccg tcgctgatct ggcaggtcat catccgccga ccgccgaagg
    11941 agatattgca gttcagcact tcgccttcca ccgtggcata aacgcccggc agtagttctc
    12001 cgatggggta gagatgggtg cgatcttcgt agcgcagggg aaggtgtaag agtagatcct
    12061 gcacggtatg caggttgatt ttcgccagtt tgttactaag tgctgcgcca acgcccgtta
    12121 gggaactgag tgggacagca tctaacaggc gacctttcat ggcacttacc ctgcagcctg
    12181 catagtagcc caccagtcgg catcagcttc gatctcgcct tgctgattga cgtggggata
    12241 aggcaggcct ttgcgttttg cgactttcgc cagcaccgga tagccgcctt caaacaacag
    12301 gcgttgttgc tctgcttccg gcaacatgct gttttcacgc aggtacatgc ctgcattttg
    12361 ccgctgacgc tgggcttcgt aaagaatgag ggctgaggca acggaaacat tcagcgactg
    12421 caccatgccg atcatcggaa tgatgatgtc ctgatccgcc agggccaatg cttcctgcgt
    12481 gatgcccgtt ttctcctgtc ccatcaaaat gcaggtcggg cgagtgtaat caatttcgcg
    12541 gaaatcgaca gcgttatcag aaagatgggt tgccagaatc tgcatgccct ggcctttgag
    12601 atgagcgacg gcatcgccaa tggtgcggtg tgttttcacc tgtacccagc tgttactacc
    12661 cgccgctgcc gaagccatgg tgcgcatgcg gctaccaggc cagacggcgt gaacttcatg
    12721 tacgccaacg gcatctgcgg tacgaataat cgcagaaacg ttatgaggtt tgtggacctg
    12781 ctccatgcag acggtcagat caggctgccg cctggcgagc atttcgcaga tgcgtgcata
    12841 acgtgttggg ttcataaaac attaatttcg gtttcgggtg actttaatca cgtctggcat
    12901 cacgcggatt ttgcgcatga tattcgccag atgcacacgg tcacgggcgg tcagacgaat
    12961 aaaggcgctg tagacgcgac catctttctc ttccgtattc aaactttgaa tattcgaagt
    13021 cgtggtgtta attgccgccg tcaggtttgc cagcgcgccc tgatgattga acatctccac
    13081 cttgatttcg gtgatgaact cctgcgccgt ctctttatcc cattccacag ccataaactt
    13141 ctctggctct ttctggtagc cacggatatt acggcaggat tcatggtgga tcaccagacc
    13201 tttaccgggg ctgacgtggg cgataatcgg gtcgccagga atagggcggc agcatttcgc
    13261 aaaggtgatc agcacgccat cggcaccttt aatgggcaga tgtccgtggc tttgggttgc
    13321 cggtggaatg gaggcgtccc catgttgcag atttttcgcg accaccacgc tcattgcgtt
    13381 accaagtccg atttctgcca gcagatcgtc aagcgttgcc agcttcatgc gatccagctc
    13441 gcgctgaata ttttcctgcg ggatttcatt gagcttacgg ctaccaccca aagcatggtt
    13501 gagcagacga cggcccaggc ttacagaatc atcacgcttg aggtttttca gcaactgacg
    13561 aattttggcg cgcgctttcg agctaacgac aaagttcagc caagcggcat tcgggcgagc
    13621 gcccggagcg gtaatgattt caacggtctg accgctagta agcggctgcg acagcgggta
    13681 aggctggcgg tcgacgcgtg cgcccacgca ggcatggccg atatcggtat gcactgcata
    13741 agcgaagtcg acgggcgttg caccggcagg cagctcgaca atgcgccctt ccggtgtgaa
    13801 aacgtaaatc tcatccggga agagatcgga tttaacgctc tcgataaatt caaacgaact
    13861 accggcgctc tgttgcagct ccagcaggct ttgcatccag cgctgggcgc ggatttgtgc
    13921 ggtagtactg gtttcgccgt gctctttata agcccagtgc gcggcaacac ccatctccgc
    13981 catctgatcc atatcttcgg tacggatctg gacctcaacc ggcacgccgt gcgggccaat
    14041 catcgaggtg tgcaacgact gatagccgtt cgctttagga atggcgatat agtctttcac
    14101 gcgacccgga cgcggcttgt acaggctgtg catctggccc agcacgcgat aacaggtgtc
    14161 agaatcattg acgatcacgc ggaaagcgta gatgtccatg attgagtgaa aacgctgctc
    14221 tttgagcacc attttgcagt aaatcgaata aagatgcttt tcgcgaccac tgacgcggca
    14281 cggtattccc gcttcctgca aacgcccttc gatttcagag aggatttttt ggatcatctc
    14341 tttacggttg ccgcgcgcgg ctttcaccac ttctttaatt acgcggtaac gattgggata
    14401 cagcgcctca aaacccagct cttcgagttc ggttttaatg tggtggatac ctaaacggtg
    14461 cgccagcggg ctataaattt cgagagtttc acgggcgatg cggcgacgtt tgtccgggcg
    14521 aagtgagccc agcgtgcgca tgttgtgggt acggtcggca agtttgatga ggatgacgcg
    14581 gatatcctgc accatcgcca taatcatctt gcgaaagttt tcggcctgcg cctctttctt
    14641 atcgcggaac ttgagtttat caagtttcga caccccctct accagctcgg cgacgctttt
    14701 accaaaaagc tgttccatat cctggtaggt ggcgggagta tcttcaatca cgtcatgcag
    14761 cagcgccgcc atcagcgttt catagtcgag tttcatctcg gccagaatgc aggcaaccgc
    14821 taccgggtgc gtgatatagg gttcaccgct tgaacgtgtt tgcccctcgt gagcatcacg
    14881 tgcaacgaga tacgcctgcc gcagacgctt gatttggtct tccggcaggt aggtttgaat
    14941 cagttgattc aggctttcaa acagatacaa gggcgacccg ctttgtgatt aacgacgacc
    15001 ttcagcaata gcggtaacgg cttgtaattc agcggcttcc tgctcttgct gttcctggcg
    15061 ttcgcgaacg tcgaggatct ggttgttgat cagaccttct tcgatttcgc gcagcgcgat
    15121 tacagtggtt ttatcgtttt cttccggtac cagcggatcc tttccgccta cctgcatctg
    15181 acgagcgcga cgcgcggcga ccagtaccag gtcaaaacgg ttaccaattt tctctacagc
    15241 gtcctgaaca gttacgcgtg ccatacttaa aaagctccac aggtgaagaa atgactgggc
    15301 atgatactga aatcaggttc agtctgccaa caatttgctg attaaagcgt catgacgctg
    15361 cttttggcgg ctcatgcgca gacgttcggc gcgaataatg gtcttcaaat cggtcaacgc
    15421 ggtatcgaag tcatcattca caatcagata atcatattcg gcgtaatggc tcatttctgc
    15481 aacagcttgc gccatacgct ttgcaatgac ctcttcgctg tcctgaccgc gaccgcgtag
    15541 acggcggtcc agttcaattt tggacggcgg taaaataaag atactccgcg cgtgcggcat
    15601 cttctggcga atttgctgcg cgccctgcca gtcgatatcg agaaaaacat cgacaccggt
    15661 cgccagtact tgctcaatgg cctcacgcga agtgccatag taattaccaa aaacttctgc
    15721 gtgttcgagg aacgcatctc tgctaatcat ttctttaaat tcatcatgat taacaaagaa
    15781 ataatgttca ccgtggactt caccaggacg cggttggcgt gtggtgtgtg aaacagaaac
    15841 ctgggtgtca tacaacggtt gggtttttaa taaagcctga atcaggctgg atttacccgc
    15901 gccactgggg gcagaaacaa tataaagcgt gccttgagcc atgagtatct ttcgtatgtg
    15961 attagcgaaa taaagcctac atacgagctt attatacacg gcgcggccat gtgacgtagc
    16021 ccttgctcac actttttgcg caaagttgtt gcgttttgcg tagctgtttc cgcatttgcc
    16081 tgtgatttac tgcaataaag acgattctca ggaagcgtcc tcgcagactc cgtttttgcc
    16141 actcggcttt gccttcacca cgttgtatat ctgccagcct gactacagcg gaggtgaaga
    16201 tgaaagtatg gatggcgata ttaataagta tcttgtgctg gcaatcatct gtgtgggcgg
    16261 tctgtccggc ctggtcgcca gccagagcac aggaagaaat ttcccgcctg caacagcaaa
    16321 taaaacagtg ggacgatgac tactggaagg aaggaaaaag tgaggtggaa gacggtgttt
    16381 acgatcagtt aagcgcccgt cttacgcagt ggcaacgctg ctttgtgagc gagccccgcg
    16441 acgtgatgat gccgccttta aatggtgcgg ttatgcatcc ggttgcccat acgggtgtac
    16501 gcaaaatggc ggataaaaac gcattaagtc tgtggatgcg agaacgtagc gatctttggg
    16561 tgcagccaaa agttgatggc gttgcggtaa ccctggttta tcgggacggg aaactgaaca
    16621 aagcaatcag tcgcggtaat ggcctgaaag gcgaggactg gacgcagaaa gttagcttaa
    16681 tttccgctgt gccgcaaacc gttagcgggc ctttagccaa cagtacgctt cagggggaaa
    16741 tatttctcca gcgcgagggg catatccaac aacaaatggg gggaataaat gcccgcgcaa
    16801 aagttgctgg cctgatgatg cgccaggacg atagcgacac gctgaattct ctgggcgttt
    16861 ttgtctgggc atggccggat ggaccgcagt taatgaccga tcgtttaaag gagctggcta
    16921 ccgcaggttt tactctaacg cagaggtata cccgtgcggt taaaaatgct gatgaggttg
    16981 cgcgcgtacg caatgagtgg tggaaagcga aattaccctt cgtcaccgat ggcgtggttg
    17041 tgcgaggggc gaaagaaccc gaatcccgcc attggctacc aggccaggca gaatggctgg
    17101 tggcctggaa atatcaacct gtagctcagg ttgccgaagt gaaggcgatt cagtttgcgg
    17161 tgggtaagag cggtaaaata tcggtggttg cgtcactcgc acctgtcatg ctggatgata
    17221 aaaaagtcca gcgggtgaat attggttccg tcaggcgctg gcaggagtgg gatattgcgc
    17281 ctggtgatca gatcctcgtc agccttgccg gtcagggtat tcctcgcatt gatgatgtgg
    17341 tctggcgcgg tgcagaacgt acaaaaccga caccaccaga aaaccgcttt aactcgttga
    17401 cctgctactt tgcttctgat gtttgtcagg aacagttcat ttcacgctta gtctggctgg
    17461 ggtcaaaaca ggttctgggg ctggatggca ttggtgaggc cggctggcgc gcgctgcatc
    17521 agactcatcg ctttgagcat atcttttcct ggcttttatt aacgccagag caattacaga
    17581 acacgccggg gatcgcgaaa agtaaaagtg cgcagctatg gcatcagttt aatctggctc
    17641 gtaagcagcc ttttactcgc tgggtgatgg caatgggaat accgctaacc cgggcggcgc
    17701 ttaatgccag tgatgaacgg tcctggtcgc aacttttatt tagcacggag cagttctggc
    17761 agcagctgcc ggggactgga tcggggcgcg ccagacaggt tattgaatgg aaggaaaatg
    17821 cgcaaatcaa gaagctgggc agttggctgg ctgcccagca gatcacaggt tttgaacctt
    17881 agtgtccttc gtggctgtag taaaagaccg gtaatcccag cttaaggcgt agcgccagta
    17941 agcgggcaaa aaagccgaat accagggtgg agataattac cacatcatgg ttagaaacat
    18001 agtgttgcag cgcgatgtac agcacggcgg aggcaaatga gacaccggca tacaactctt
    18061 tctggaatac cagtgggata cgtttacaga acatatcgcg caaaacgccg ccaaacacgc
    18121 cggtggttac cgccgcgaca acggcaataa ttgggccgtg ccccatatcc agtgcaacct
    18181 gtgcgccgat gatagaaaag accaccagtc caagcgcatc gagcaccaga aacactttgc
    18241 gcaggtaagg cattacagga gcaacgatag tggtgagtac tgcggcggta gcgacgataa
    18301 tcacatattc cgggtgtttg acccagccga gcggatagtg gcccagcaga atatcgcgca
    18361 ctgaccctcc gccaattgcg gtggcggtcg caataataat tacgccaaat gtatccatgc
    18421 ggcgtcgccc ggccgccaga gcccccgtca tggcttcggc agtaatgcca accaaataca
    18481 aaatgtgtaa cagcatggtt ctctccctaa aaatctgtct ggcagggtag cgatttgtgc
    18541 gtattgtcac gattgagatt ttttaaggtg agaggaaatt gataacttgt agttatgtga
    18601 aatcgctatt ttctgtagcg tagatgcatt cttctgactt cttgtctgag aaaatgcata
    18661 agaaaaaata ggttaaaact gaggggggaa aagaaaggtg caatgtttag ccattgtgtt
    18721 cactgcacct ggaccttggc ttacgcatcg ggtgtctcaa tcaacaaaaa gagtttttcg
    18781 tttctgctat ttcttctctg taatttttac actgttttcc aactgcttga tgctgacctg
    18841 cggggtcggc aactcctcag gcatggttcc gccaagttcc tgaatggttt gcctcacctt
    18901 gctacccacg tcaaagtgtg tggtattagc ttgctgtgct gcctcaacca attgtttgtt
    18961 atgttctttc aactcatttc tcagaaacag acgcttttcg tcttcacgaa gttgcttaaa
    19021 tgcttcatcg tcagcaagct cctgccgtcg ggtctggata gcaaaataag tctgccctgc
    19081 cgcaatgacc ggtttcgcgg ggtcgccgtt ttgtactacc aggtaacagg catagcggga
    19141 gagtacgatg tcttttaatg ctcttttggc atttgagcca attttgacca tatcgaggat
    19201 ctcctcgaaa tggtcatcaa ttgtgtgacc actgttctca caggcctctt tggcgcgcgt
    19261 taatacaggt ataaagtgac gatactctga ataacccagt agtttcccaa gttgtcttgc
    19321 tgaccattgt tctgctccgt ttgcattaat cagttttatc tcttcaaaag gttgatgatg
    19381 ttcgttcatt gcccactcca tgtgatatcg attgcaaaga aagtaaatct gtaactgtat
    19441 ttatatacag ttgtgagtat ctcacgttct cagtgtgctg ggcaaaaaaa catcgcaaaa
    19501 tctggagcag gcacgcaaaa cagaattgaa gttgcgggga agaaaagtgc cgctctgttt
    19561 gagtgggcgg catttgtgaa ggctgaaaag taagaattag tgttacgagt tactcaatat
    19621 tctgaatctg ctcgcgcatc tgctcaatca acactttcag ctcgatggcg gagtttgtca
    19681 cttcggcatt gatagatttc gacgcaagag tgttcgactc gcggttgaat tcctgcatca
    19741 taaagtccag acgacgacca acggcttctt ttttcttcag aatgttgtag gtctctttga
    19801 catgcgcttc caggcggtcc agttcttcgg caacgtcaat tcgttgtgcc agcagaacca
    19861 actcctgctc cagacggttg ttttccaact gaacctgagc atcttccagc ttcgcgacca
    19921 gacgctcacg ctgccattgc aggatttccg gcatatgggc gcggactttg accacttcgg
    19981 cggtgacgcc ttccagacgc tgctcgatca atgctttcag cgcctgacct tcggtttcgc
    20041 gcgcgacgat aaagtcgtcc agggtaccat ccaacgccgc gagaatttca gcggcaatcg
    20101 cgtcgagatc ctgctcctgg gctgccatca cgcccggcca gcgtagaata tcaaccgggt
    20161 tgatttcccc ttcgtcactc tgcattttta cccagttcgc ggcagttacc agctgtttag
    20221 ccagtttttc gttgaggatc agctcacctt gtgcgctaac atctggctca tagcgcaggg
    20281 tacattccac tttaccgcgc gtcaggcgag aacgaatacg ctcgcgaacg acaggttcaa
    20341 ggctacggaa ctgctccggc agacgaaagt aagtttccag ataacgctgg tttaccgagc
    20401 gcatttccca ggttgcgctc ccccattcac ccttgatttc acgccgggcg taggcggtca
    20461 tactgcggat catagacgtt cctgtttata aaaggagagg tgggaggatt atagccatcg
    20521 atgccttgtc aggataggaa taaccgccgg aagtccgtat aatgcgcagc cacatttgtt
    20581 tcaagccgga gatttcaata tgcgtccagc aggccgtagc aataatcagg tgcgtcccgt
    20641 taccctgact cgtaactata caaaacatgc agaaggctcg gtgctggtcg aatttggcga
    20701 taccaaagtg ttgtgtaccg cctctattga agaaggcgtg ccgcgcttcc tgaaaggtca
    20761 gggccagggc tggatcaccg cagagtacgg tatgctgccg cgttctaccc acacccgtaa
    20821 cgctcgtgaa gcagcgaaag gtaagcaggg tggacgcaca atggaaatcc agcgtctgat
    20881 cgcccgtgca cttcgcgcgg cagtagattt gaaagcgctg ggtgagttca ccattacgct
    20941 ggactgcgac gtgcttcagg ctgatggtgg cacgcgtacc gcttcgatta cgggtgcctg
    21001 tgtggcgctg gcagatgcgc tacagaagct ggtggaaaac ggcaagctga aaaccaatcc
    21061 aatgaaaggg atggtagccg cggtttctgt cggtattgta aacggtgaag caatttgcga
    21121 tctggaatac gttgaagact cagccgcaga gaccgacatg aacgtagtga tgaccgaaga
    21181 cgggcgcatt attgaagtgc aggggacggc agaaggcgag ccgttcaccc atgaagagtt
    21241 actcaccttg ttggcgctgg cccgaggggg aatcgaatcc attgtagcga cgcagaaggc
    21301 ggcgctggca aactgatttt taaggcgact gatgagtcgc cttttttttg tctgtagaaa
    21361 agtaagatga ggagcgaagg catgaaacca tatcagcgcc agtttattga atttgcgctt
    21421 ggcaagcagg tgttaaagtt tggcgagttt acgttgaagt ccgggcgcaa aagcccctat
    21481 ttcttcaacg ccgggctgtt taataccggg cgcgatctgg cactgttagg ccgtttttac
    21541 gctgaagcgt tggtggattc cggccttgag ttcgatctgc tgtttggccc tgcttacaaa
    21601 gggatcccga ttgccaccac taccgccgtg gcgctggcgg agcatcatga ccttgacctg
    21661 ccgtactgct ttaaccgcaa agaggcaaaa gaccacggtg aaggcggcaa tctggttggt
    21721 agcgcgttac aaggacgcgt aatgctggta gatgatgtga tcaccgccgg aacggcgatt
    21781 cgcgaatcga tggagattat tcaggctaat ggcgcgacgc ttgctggcgt gttgatttcg
    21841 cttgatcgtc aggaacgggg gcgcggcgag atttcggcga ttcaggaagt tgagcgtgat
    21901 tacaactgca aagtgatctc tatcatcacc ctgaaagacc tgattgctta cctggaagag
    21961 aagccggaaa tggcggaaca tctggcggcg gttaaggcct atcgcgaaga gtttggcgtt
    22021 taaagaaact cgccggatga aaagtcatcc ggcgtcatat tactgcaact gtgccgcaat
    22081 tagcggccag cgggcgtcaa aatcatccgt cgggcggtat ttaaattcgc tgcggacaaa
    22141 acgtgacagc ataccttcac agaaggccag gatctggctt gccagcaggg tttcatcggt
    22201 ggtgtaacct tcaccctcac gcattctctt ttcacgcaat acctgacgca gctgcgcttc
    22261 aatacgctcg aacagctggt tgatgcgccc ttgcaggcga tcctgttcaa acattagcgc
    22321 atgaccagtg aggatgcggg tcaggccagg attacgctca ccaaaaccga gaagcagcaa
    22381 cacaatcaga cgcaggcgcg ctgtggtgtc tttctcatct ttcagaatca gattgatgcg
    22441 agtaatcagg ctatcttcga taaactcaat caggctatcg aacatgcggg tcttactggg
    22501 gaagtggcga tacagtgccg cttcggaaac gccgacagag gcggccagtt ttgccgtcgt
    22561 gatacgttgg cttccatcgc tggattccag catcagcgcc agagactgaa gtatttcctc
    22621 gcgacggttc cttttcgcag tttgtttttc tgccatgtta caaaataccc ctgaaaataa
    22681 gcacttgcca ggcggcaccc acgctatgac cgcaaacgaa atgtttgcgg ctatgttatg
    22741 acgttattcg gatgcgtatg tgttactgac gaccagagtg accaaagccg ccttcaccgc
    22801 ggtcggtggc gtcgaaatct tccaccagat taaattcagc ctgtactacc ggaacaaaaa
    22861 tcatctgggc gatgcgttcg ccaggttgaa tggtgaagct gtcctgacca cggttccaca
    22921 cggaaatcat caactggccc tgatagtcgg aatcgatcaa tcctaccagg ttaccaagca
    22981 cgataccgtg cttatgtccc aatccggagc gcggcagcat cattgccgcc agtgaaggat
    23041 cggcaatatg aatcgccagc ccggtcggaa ccagcgtagt gtcacccgga gccagttcta
    23101 cggcgtcgtc gagacaggca cgcaggtcaa gtccggcaga gccagaggtg gcataagtcg
    23161 ggagcggaaa ttccttccca acgcgcgggt ccagaatctt aacgtcgatt tttttcatca
    23221 taacgggtca cgatctcgtc gagtaataat tggccaagga gctctttgcg ctcaagcggt
    23281 aagactttat ctccgtcctg ccagaaaagg tgtaatgcgt tgttgtcgct gttaaatcct
    23341 tgagttggct gggaaacatc gttcgcgcag atcagatcaa ggtttttacg gatacgtttt
    23401 tgccgggcgt attcttccac attatttgtt tcggcggcaa atccaacgac gtagggtcga
    23461 tggtctttta gtgcggcaac gcctgcgacg atatcggggt ttttaaccat ttttattgtt
    23521 aattcatcac cctgcgtggc ctgctttttg attttctctg gggccacggt agctgcgcga
    23581 taatccgcca cggcggcgca accgataaaa atattttgct gctgtacaga agcattcacg
    23641 gcggcttcca tttccagcgc agtcatcaca tcaacacgtt taacaaacgg tggcgtcggt
    23701 agtgaaaccg gacctgatac cagcgtgacg ttcgcgccac gacgggcagc ggcggcggcg
    23761 atagcaaaac ccatcttgcc ggagctgtga ttagagatat aacgcaccgg atcgagcggt
    23821 tcacgcgtcg ggccggcggt aatcataatg ttcagatgtt tcaggtcgtt gacgggcgaa
    23881 aaatgcgcca ccgccatatc cacaatggtt aacggatcga gcattcgccc aggaccgata
    23941 tcaccacaag cctgactgcc actgtctggc ccccagatga gcataccacg ggaagcaagc
    24001 acctctaaat tatgctgcgt ggcagcggca cggtacatct gctggttcat ggcggggagc
    24061 acggctacag gcgcaggtgt agccagacaa atcgtcgata ccaggtcatt cgccattccg
    24121 gcagcaatac gggcaatcaa atctgccgtg gcaggggcga gaatcactaa atcagcccat
    24181 ttacccagct caatatggcc catagcggct tcggctgccg ggtccagcag actgtcggaa
    24241 acgggataac cagaaaccgc ctgcaagcta agtggggtga taaaggcttt tgccgcttcg
    24301 gtcatggcta cgcggacgtc ggccccgcga tcgcgcaaac gacgcaccag ttcaggggtt
    24361 ttataggcag caataccgcc gctaacgccg agaacgattt ttttaccggc caggctcatg
    24421 atgatttttc ctgtagggtg acaccagaag ttggcgattt tatcacaata cttttgttgt
    24481 cgtgccttca cctgagattc actttgcgag gcgctttcca ggattgaaaa ctggccgtcg
    24541 atttaacgaa acggctatga caggatgcga gcaccacaaa ggaggtgaag gtgaaaaaca
    24601 attcacagct gttgatgccg cgcgaaaaaa tgctgaagtt tggtattagc gccttaacgg
    24661 atgtcgagct gctggcgcta tttctgcgta ccggaacgcg cggtaaagat gtattaaccc
    24721 tggcaaaaga gatgctggag aatttcggct ctctttatgg cttgttaacc tctgaatatg
    24781 aacaatttag tggcgttcat ggaattggcg tggcgaaatt tgcccagtta aaggggattg
    24841 ctgaactggc gcggcgttac tacaacgtgc ggatgcgtga agaaagccct ttactcagcc
    24901 cggagatgac gagggaattt ttacaaagcc agctcacggg tgaggagcgg gagatcttta
    24961 tggtgatctt tctcgactcc caacaccggg ttataacgca tagccgtctt ttttccggca
    25021 cgctaaacca tgttgaagtc catcctcggg aaattatccg cgaagcgata aaaataaacg
    25081 cctcggcgct gatccttgca cataatcacc cttcgggttg tgctgaaccc agtaaagcgg
    25141 ataaactcat tactgaacgg ataataaaga gttgtcagtt catggattta cgcgtgctcg
    25201 accatatcgt gattgggcgt ggagagtatg tttcttttgc cgaacgcggc tggatttaac
    25261 ccgctatgcg cgatccttcg ggatctttgt ctgttcggga cttgagcaca tcgctgagtc
    25321 agcgtatact acgccacctt tgagaatctc gggtttggca tttgggcctg gcaatcgaga
    25381 gttcacatag aactgcgatg accgggctgt aaagcctgac gaggcgccaa taccccatac
    25441 gaagctcgag ctaatttgat ttttggagaa tagacatgtc ccgagtctgc caagttactg
    25501 gcaagcgtcc ggtgaccggt aacaaccgtt cccacgcact gaacgcgact aaacgccgtt
    25561 tcctgccgaa cctgcactct caccgtttct gggttgagag cgagaagcgt tttgtcaccc
    25621 tgcgcgtatc tgctaaaggt atgcgtgtaa tcgataaaaa aggcatcgat acagttctgg
    25681 ctgaactgcg tgcccgtggc gaaaagtact aagtacttag aggaaataaa tcatggctaa
    25741 aggtattcgt gagaaaatca agctggtttc ttctgctggt actggtcact tctataccac
    25801 tacgaagaac aaacgtacta agccggaaaa actggaactg aaaaaattcg atccagttgt
    25861 tcgccagcac gtgatctaca aagaagcgaa aatcaaataa ttctcgcttt gatgtaacaa
    25921 aaaacctcgc tccggcgggg ttttttgtta tctgcttgcc cccatattga ctgcatctgt
    25981 tcattcctgg agatgctatg cctgaattac ccgaagttga aaccagccgc cgcggcatag
    26041 aaccgcatct cgttggtgca accattcttc atgcagtggt gcgcaacgga cgcttgcgct
    26101 ggccggtttc agaagagatc taccgtttaa gcgaccaacc agtgcttagc gtgcagcggc
    26161 gggctaaata tctgctgctg gagctgcctg agggctggat tatcattcat ttagggatgt
    26221 ctggcagcct gcgcatcctt ccagaagaac ttccccctga aaagcatgac catgtggaat
    26281 tggtgatgag caacggcaaa gtgctgcgct acaccgatcc gcgccgcttt ggtgcctggc
    26341 tgtggaccaa agagctggaa gggcataatg tgctgaccca tcttggaccg gagccgctta
    26401 gcgacgattt caatggtgag tatctgcatc agaagtgcgc gaagaaaaaa acggcgatta
    26461 aaccgtggct gatggataac aagctggtgg taggggtagg gaatatctat gccagcgaat
    26521 cactgtttgc ggcggggatc catccggatc ggctggcgtc atcactgtcg ctggcagagt
    26581 gtgaattgtt agctcgggtg attaaagcgg tgttgctgcg ttcgattgag cagggtggta
    26641 caacgctgaa agattttctg caaagtgatg gtaaaccggg ctatttcgct caggaattgc
    26701 aggtttacgg gcgaaaaggc gagccgtgtc gggtgtgcgg tacgccgatt gtggcgacta
    26761 aacatgcgca gcgggcaacg ttttattgtc ggcagtgcca gaagtaattc atgcgcgccg
    26821 gatggcatac catccggcat aaacgctacg ctaacttcgc catcagcgcc tgatggacat
    26881 tctccggcag gaaatgggtg acatcgccct gatggcgcgc cacctctttc accaacgatg
    26941 aagagataaa cgaccactct ttcgacggca tcagaaacac actttccagt tccggcatta
    27001 agtggcgatt catatgcgcc agctgcattt catattcaaa atccgccacc gcacgcaggc
    27061 cacgaatcag caccgtagcg tgttgattac gggcgaagtt cgccatcaaa tcactaaacc
    27121 cgaccacttc cacgttcccc agatgcgcgg ttgcctgctg tgccagcgcc acacgctctt
    27181 ccagggtaaa catcggtttt ttactggggc tggcggcaat cgccagaata acgtgatcga
    27241 acatttgcgc ggcgcgcgtc acgatatcga tatgaccatt ggtaatggga tcgaaagtac
    27301 ccggataaat cgcccgtttt tgcataacaa cctcaatgcg ttttcggtgg caggtaaggt
    27361 tccagcagtt gaagcagacg ctgtagcgcg ccctggtttt gatacagtac ttcaacggca
    27421 tgacggccat agaaactacg gtaatcggcg tcggtgagta aagaggacac ctcttttgca
    27481 agcgtagtgg catcggtaac ggtaatcagc ccgcttgcct gctccagccg cgcgcaaatg
    27541 tctttaaagt taaaagtatg cggccccatc aataccggaa tagcgtgtgc ggcagcttcc
    27601 agcggattat gcccaccacg ttcaaccagt gaaccgccaa caaaggcgag atcggcaatg
    27661 ccgtacagta acatcaactc gcccatcgta tcgccaacta caacctgcgt gctggtggag
    27721 gggacttccc ctgaagagcg tgtgatatag cttagtccag cctggcggac aaggttaatc
    27781 gcatccggga agcgttctgg atgacggggt accaggatga gcaataaatt cgggaattgc
    27841 tgtaacaatg cctgatgtgc ggcaatgacc acgctctctt caccttcgtg agtgctggtg
    27901 gcaatccata ccgggcggtg tggtgcccat tggcggcgta gcgtcacggc tttagcagcc
    27961 aactgcggcg ttacagaaat atcgaatttc aggctaccgg taacggttac ctggttattt
    28021 tttgcgccca gcgccacaaa acgtgcacca tcttcttcat tttgcgcagc aatcagcgta
    28081 atacgacgca gcaagcggcg gacgaattta cccagtttgg cataacctgc ggccgagcgg
    28141 gcagagagtc gcgcgttagc gatcaccagc ggaattttac gtttatgtag cgccgcaatc
    28201 aggttaggcc atagttcggt ttccataatc aacaccagtt tagggtcgac tttattcagg
    28261 aaacggttga gtgcatcggg caggtcatac ggcagataaa cgtgctgaac atccttcccg
    28321 aaagccgatt gtacgcgctc cgaaccggtt ggcgtcatgg ttgtaacggt aatcggtaaa
    28381 tcaggataac gatgacgcag cgcgcgcacc aacgggatcg ccgccagagt ttcaccgacg
    28441 gagacggagt gcagcataat gccgcctggt tttagcggat ggcggtaaaa accgtaacgt
    28501 tcaccccagc gttttcgata ggccggagcc ttacgtccgc gcacccagag ccgtatccag
    28561 atcagcggct gaataaggta gagaagggcg gtgtaaagca attcgagcat agtaaatagc
    28621 tgacttatgg atgtgctggg gattctatgt atttagctgt ggctttacca ttacttttcc
    28681 cgtttttgac ttaaatagca atttacccgc caattcttgc tgaacatcta tacttttttt
    28741 gtatttttat gcgattcatt gatttctgct gtacgtgcag gaaggaatcg cagagcaaca
    28801 gtgaaagaat atggttgcta atgattttat ttgattgatt tctttattga aaatcaacaa
    28861 tgtgcttaaa aatcattcgg gtcggattgc cgcgaaagaa gtgatacact agcgcgtcaa
    28921 tatcagtgcg gtatcagtat tcaggtagct gttgagcctg gggcggtagc gtgctttttc
    28981 tgcttcacct taatcggata atctcaacaa aaagagttac ttgtggataa gccatttcga
    29041 agaattttgc tcattaagat gcgttttcat ggggatatgt tattaactac tcccgtcatt
    29101 agttcgctga aaaaaaatta ccctgacgca aaaatcgatg tgctgcttta tcaggacacc
    29161 atcccgatcc tgtctgaaaa tccagagatt aacgcgctct acggcataaa aaataaaaaa
    29221 gcaaaagcct cagaaaaaat tgccaacttt tttcatctca tcaaggtatt acgtgccaat
    29281 aagtatgacc ttatcgtcaa tcttaccgat caatggatgg ttgctatact ggttcgctta
    29341 ttaaatgctc gtgtgaaaat ttcccaggat tatcatcatc ggcagtctgc tttttggcgt
    29401 aaaagtttca cccatttggt gccgttgcag ggtggaaatg tggtggaaag taacttatcc
    29461 gtgctgacac cattgggact tgattcgttg gtgaagcaga caaccatgag ttacccgcct
    29521 gcaagctgga aacgtatgcg tcgcgaactt gatcacgctg gtgttggaca aaattatgtg
    29581 gttatccaac ctacggcgcg gcaaatcttc aaatgctggg acaacgccaa gttttccgct
    29641 gtgattgatg ccttacatgc tcgtggttat gaagtcgttc tgacgtccgg cccagataaa
    29701 gacgatctgg cctgcgtcaa tgaaattgcg cagggatgcc agacgccacc agtaacggcg
    29761 ctggctggaa aggtgacctt cccggaactt ggtgcgttaa tcgatcatgc gcagctgttt
    29821 attggcgttg attccgcacc ggcgcatatt gccgctgcag ttaatacgcc gctgatatcg
    29881 ctgtttggtg cgacagatca tattttctgg cgtccctggt caaataacat gattcaattc
    29941 tgggcgggag attaccggga aatgccaacg cgcgatcagc gtgaccgaaa tgagatgtat
    30001 ctttcggtta ttccggcggc agatgtcatt gctgctgtcg ataaattact gccctcctcc
    30061 acgacaggta cgtcgttatg atcgttgctt tttgtttata taaatatttt ccctttggcg
    30121 gtttgcagcg tgattttatg cgtattgctc agacagtcgc cgcccgaggt catcatgttc
    30181 gggtttatac ccagtcgtgg gaaggcgaat gccctgatgt atttgaactg atcaaagtgc
    30241 cggttaaatc gcataccaat cacgggcgca atgcggagta ttttgcctgg gtgcaaaaac
    30301 atttacgcga acatcccgtc gataaagtcg ttggattcaa caaaatgccg gggctggacg
    30361 tttattatgc cgctgatgtt tgttatgccg agaaagtagc gcaggaaaaa ggctttttct
    30421 atcgcctgac gtcacgttat cgccattatg ccgcctttga gcgggcaacc ttcgaacagg
    30481 gtaagccgac acagttgctg atgctgacag ataagcaaat cgccgatttc cagaaacatt
    30541 atcagactga agcggagcgt tttcatattc tgccaccggg gatttatcct gatcgtaaat
    30601 atagccagca gccagccaat agccgtgaaa tcttccgtaa gaagaatgga ataaccgaac
    30661 aacaatattt attgttgcag gtcggttcag acttcacgcg taaaggtgtc gatcgttcca
    30721 ttgaagcact tgcttcgtta ccggattcgc tgcgccacaa cacattgcta tatgttgttg
    30781 ggcaggataa accgcgaaaa tttgaggcgc tggcagaaaa acgcggcgtg cgcagtaatg
    30841 ttcacttttt ctcggggcgc aatgatgtct cggaattaat ggcggcggcg gatttattac
    30901 tgcatcctgc ctaccaggaa gcggcgggaa ttgtgctgct ggaagcgatt actgcaggat
    30961 taccggtact aacaacagcc gtttgtggct atgcgcatta tattgtcgac gctaattgcg
    31021 gcgaggctat tgctgagcca ttccgccagg aaacattgaa tgagatttta cgcaaagcgt
    31081 taacgcaatc ttcattgcgc caggcctggg cggaaaatgc gcgacattat gctgatacac
    31141 aagatttata cagtctgcca gagaaagcgg cggatatcat aacgggtggt ctggatggtt
    31201 gaacttaaag agccgtttgc cacgttatgg cgcggcaaag atccttttga ggaagttaaa
    31261 accttgcagg gtgaggtatt tcgtgaactg gaaactcgcc gtactctgcg ctttgaaatg
    31321 gcgggcaaaa gctattttct caaatggcat cgcggcacga ccctgaaaga gataatcaaa
    31381 aatttactct cattgcggat gccagtatta ggcgctgacc gcgaatggaa tgcgattcat
    31441 cgactgcggg atgtcggcgt tgatactatg tatggggtgg catttggcga aaaaggcatg
    31501 aatccgctga ccagaacttc atttattatt accgaagatc tgacaccaac cataagtctt
    31561 gaagattaca gtgctgactg ggcgactaac cctccagatg ttcgcgtaaa gcgtatgctt
    31621 attaagcgtg tcgcgacgat ggtgcgcgat atgcatgctg cgggcattaa ccaccgtgac
    31681 tgttatatct gtcatttcct gctgcacttg cctttttccg gtaaggaaga ggagttaaaa
    31741 atttcggtaa ttgacctgca ccgggcgcag cttcgcacgc gcgttccacg tcgttggcgg
    31801 gataaagatc ttattgggct ttatttttct tcgatgaata tcggcctgac tcagcgggat
    31861 atctggcggt ttatgaaagt gtattttgcc gccccgctta aagacattct caagcaggaa
    31921 caaggactgc tgtcgcaagc agaagcaaaa gccacaaaaa tcagggaaag aacgattcga
    31981 aaatcgttgt aacccgtaac ttatttttgc caaaattttg gatacagaat aaatatgtct
    32041 caact
//
LOCUS       DAZGSP010000056        31865 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZGSP010000056 DAZGSP010000000
VERSION     DAZGSP010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.056"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            247..513
                     /locus_tag="ACHK74_002814"
     CDS             247..513
                     /locus_tag="ACHK74_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0221601.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            510..1100
                     /locus_tag="ACHK74_002815"
     CDS             510..1100
                     /locus_tag="ACHK74_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0221602.1"
                     /translation="MMMPVLQKVPFSGLPFSGICGYSFSAVAKSAAGRRNPCNSKATY
                     DAPCVFFCVAINATERQIMVWRVVCRAGMILFAIACYATESMVAQAGQPPGWPVFFEA
                     GIPTPVWAIAIERRNSGDSSYLLLEGDGLMATTLTPSHPEFVFVFAAVRRADRHPRIC
                     MLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            1093..1380
                     /locus_tag="ACHK74_002816"
     CDS             1093..1380
                     /locus_tag="ACHK74_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HFP0221603.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            1373..1828
                     /locus_tag="ACHK74_002817"
     CDS             1373..1828
                     /locus_tag="ACHK74_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221604.1"
                     /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPDIVWNP
                     YRRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYLDIYPQDTVPASRAAPVAREWREIPRTEPDPEITPDNSYRNYL"
     gene            1964..2284
                     /locus_tag="ACHK74_002818"
     CDS             1964..2284
                     /locus_tag="ACHK74_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HFP0221605.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            2299..4632
                     /locus_tag="ACHK74_002819"
     CDS             2299..4632
                     /locus_tag="ACHK74_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFP0221606.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNRHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDAVAKVAAVTGSLPPADPAVTAAA
                     VAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPDRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRRLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVD
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATA"
     gene            4837..5172
                     /locus_tag="ACHK74_002820"
     CDS             4837..5172
                     /locus_tag="ACHK74_002820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221607.1"
                     /translation="MSHTGRMPEVKNRMFTLHPLFTTYHYEIKGEKRKVNSVNSSSEK
                     KFFMWIKDALVWIRTTDPNLLIKNYLTFRFVDGLKIDWSEKQWGHKRGHISLLCFIIC
                     FLSLTYFDV"
     gene            5388..6653
                     /locus_tag="ACHK74_002821"
     CDS             5388..6653
                     /locus_tag="ACHK74_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0221608.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLVDARGRRDEARKLLANGVDPSENKKAVKVEHEQEVITFEVV
                     AREWHASNQKWSASHSARVLKSLEDNLFAAIGKRNIADLKTRDLLVPIKAVESSGRLE
                     VAARLQQHTTAIIRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLQRIY
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEVDFDTAMWTIPGEREPLEGVKHSQRG
                     SKMRTPHLVPLSRQALTILEKIKSMSGNRELIFVGDHDPRKPMSENTLNKALRVMGYD
                     TKTEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERMLM
                     LQWWADYLDANRMAAITPFDYAKINSSFR"
     gene            complement(6853..8322)
                     /locus_tag="ACHK74_002822"
     CDS             complement(6853..8322)
                     /locus_tag="ACHK74_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFP0221609.1"
                     /translation="MNIKSAHFLPGTNKRLGDKYLLLECLGDGSHGWVWRAERLKDGK
                     IVAVKIPKDITREDRQLAEGKELLNVEPHENIVQIFDMGRIDNEWFYIEMEYFPSQTL
                     AQKLDDRQRNFGQTYERLFRIYRQVLCAVHYLAELPVPISHGDIKPHNILVGERDLVK
                     LTDFGSSALPEEIYVRTRENGGTVLYSAPEFSNVDSRRGSLEELLLGDIYSLGVLLYQ
                     LLTGKLPHDTPAQVQRHAPFKLPTEINSSIHTDLEQVVLTCLQKRAEDRFSTISDLIH
                     AFDSAATKQLKVGAIAPVFQTKPAQDWSSAVLEAMSDQSYQKAAQLASQEYSRSKDLQ
                     ALHQQLIALYRANRLFDFEKVVEDNKAILLEGKLTQPAALFELIVKANLQLRNISQAN
                     FWLLQRKQAEAENAATMYLESSILGLEAKYSEARVLLEKVNQTTPMKFHVLSQLILVC
                     EQMRDYSGAAAYLKAALRVAPLDNSMKEKKELYAKLGVI"
     gene            complement(8319..8861)
                     /locus_tag="ACHK74_002823"
     CDS             complement(8319..8861)
                     /locus_tag="ACHK74_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221610.1"
                     /translation="MPEVAWVNETFPLLASGKLLDQDVLVHSLGCSVWNTFGHEQSFM
                     AVVECPAPGTFGADIRSDSGWFHKSSASPVCLIEFERFDGSAKGQQKLEEKLKNLLEA
                     AQRWNHCPKTLVLSAWSQGLVGVPDTQKLKDICRMGFTSSTGTQVIAAPDVEVVFSRF
                     LFIKNLNMIVLDRIHYEVLM"
     gene            complement(8869..10554)
                     /locus_tag="ACHK74_002824"
     CDS             complement(8869..10554)
                     /locus_tag="ACHK74_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HFP0221611.1"
                     /translation="MAYKVLGKLVGNTGDASQLTMVVQDSFSVRRGEFVRIMHQERKD
                     EGQVAVLGRVTKISRTNMLYNAGFGDGVTELELLPGANVTGENMFAQVELVGYRDPVT
                     RQIKIPRRPLNPGTAVETVDFQFLSDFYEFNEHSSLHLGNLVGYDKGENTVPVFIDVN
                     KLVTEHLAVLAMTGSGKSYTVGRIIERLVAVNNGTVVVFDPHGEYGKALAGGNLQFSS
                     FLEATDDKRDQEALPLIKQTFEKLQAAGAGIQVYSPQHESFKHKYASKNTPLALQFDH
                     FEMDDIAEILPGLTEPQQRVLDVAIRYWRYADKTEPRDINRLRFLLGDGIEELKEWDD
                     LSEAEAKALSGRSAAVASMKLSRVLNEAKSFYSATMAEPTDIYKMVGRPSNQQGRLVV
                     VDLQGLSDTAKQVICALLSSEILKAASSKTDPLRPCFIIYEEGHNFAPAGGNAVSHRI
                     IKKIAGEGRKFGVGFGIVSQRPSKLDSDVTSQCNTLITMRLKNPDDQRFIAKASDMVS
                     KADLEELPSLSTGEALVCGRSIPAPLLVKVGSKALIHGGESPEVLRVWGSFSG"
     gene            complement(10544..11701)
                     /locus_tag="ACHK74_002825"
     CDS             complement(10544..11701)
                     /locus_tag="ACHK74_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221612.1"
                     /translation="MNENKTMNLAQSLAIDSQLRLPGSAGRLLKQVRDIEQVAPLFRD
                     AGLVKAVPKPTIVNLRVAGIATKSCLDKVLGGFIYASAAVRTDLLIDDNKVSTVGSDS
                     VSELDDIDFEAQRKRLDLIEIRQAYQLVEKKLLGYEPGDLILLDTPLFLARDMAPLER
                     NVRHKQEYEKTRQVIETFWQNHRSRIFPWNQNGVVLASILAERFSAIVSIAKQDLRTE
                     QGRKQILASDGFSKELMQNLEGLDESLVGVGDLRFINGILGNYSRTIAFRLSEQEKRI
                     EPNIEAKQGLIGFHFRSARGGQIKMVQLAGDEPEWKSADLDLVASRLMVLDMQNKGNA
                     MPLPQLLGYQQLGILPKFATFYRQGLHQALKNNDIEAGWLAGLDEGLNNGL"
     gene            complement(11704..12552)
                     /locus_tag="ACHK74_002826"
     CDS             complement(11704..12552)
                     /locus_tag="ACHK74_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221613.1"
                     /translation="MSRYLSAALASNRKGRFLQTVAGATPLMKDWISSPPASGLLIVQ
                     AEELTDANTMQHLYHWAMQAGCAALVINLKAEQFTLLAQLPYPLDWQLVPASLRGQEP
                     GLTALLASETDQAIAGFTGSADRYQHQAGDVVHTRYIRKHSNSGLLAFTTLPLWSLTL
                     LDHSELLVSWLNWFVDHAGIAERIIEPKAPSTDYTPDKHDLVVLLLLYAGGGMNLQAL
                     SEHNAVKLMFDVNSLDIVKRGEMLRQHDFIDDAGITATGKTCLQASQYWAYAPLLGEQ
                     LHTGTL"
     gene            complement(12563..16849)
                     /locus_tag="ACHK74_002827"
     CDS             complement(12563..16849)
                     /locus_tag="ACHK74_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221614.1"
                     /translation="MSFELNEQMVDSLAKQHRWAATGVSDYPPRDPLVGQSRFFKRFQ
                     TFLHTVDHDDDRFAHVFAVEAEWGRGKSRLGHELVAQINDCSRGWFVRDEQGQLHDKQ
                     LFNRDTQDKYLALYIRYSQVASDYQNSDNWFAFGLYQALLPLATQTFDGSIQSEIAKQ
                     AYERLMPFGFKSELLADALQLNANHSEEDLYTDQTLVVSLVQAAYKVLKSFGVQYMLV
                     VLDELETVAEAATFGLEDEQNKRLDGQAIRLIGKAIKEEDPRRKLPWLRYVALCSPLL
                     GQQLREIQSVARRFELVELEHNAFADVSDYVASLLKDGKLGFNYPAGLVEAAYAMSGA
                     NFGWFNVVMANVDAVLSQYQQAGRSIKDVGDLFEAVLAGSGRVARHVLDKGAIEGIQT
                     RDQALLTECRSLLYGQLPVRLAEMAYATELLALKNEDLEPIAAHYRKLSIDRLQCRQA
                     LEQAKFRRDKDEWYYPAVDQALNLDTLLDNLRTFSIKEQAASGDKGAVLLPLSKGEFK
                     YLLSLLYDHPAIEFAADALWHKLVGETTELDASEATHIGPSVAMLLRLDLRYRSAQQN
                     SLIFKDPAMGDAHEQAMSELSKQSSQKPLIKALARVTGFVRLLDNNWTYDENLLSNKA
                     GSDDAPLAILTEPRGRQGGLLTLDGFKLHPEGKALFAWVSNLIELNNLHTFAANHSHQ
                     NGRTPVLALTASAHLMEQYSRLDERNELRDAILLHYVNPSEADQLERIGLSLAACQLH
                     GVNLTADSFTAKFKNKLHALTTFATDAIHKWRQRLQQRGLIAWPLKVDGKLSSNDRDL
                     LFKGWYQLAIAHPELNGILDLQQQHGVPVNELSSLLDKLKVPGSYIAKGYTADEHAGL
                     FTELNNVQRSQAQIPLFLARIAHPNKKHKWQFEGFKQQFYFAYVAETSVTAKGVFNDW
                     MWWCGELNLLTLTNPTEKQAIWEHYPRSRLENAIREAQNWFRGNDMGSYAANVEVMSR
                     VYGYARINEMFAPLGKNKLGFVTVEAKEQLEKAQSLFNVLKQQEEQLADMVEANDTKV
                     LAGLIHKRAEVLELVAKVKPLNSSRPMLKDAHILSLEDKTTSLYQRIEQACLFAEFVE
                     CSAERINNRLVDLIIDVETECAPLTNFPKRLYTNTLRTIGHILDGALKDDTSSATGRK
                     EQQADSDTLLHYLRKLDLGRAHDKLSALAQEVGLNLHNDQQLPIAEIQGHILSSYRNC
                     KERFSKLVNNLTEQKLRAQQLQEWLSSATAEYQFADDIAELPKLVMKLQLIEDAIADL
                     PNDAESKRQSMQNSLRNGQFSSLRDLPEELLKPARGQLTPIQGQLLKIEERLNQVRRN
                     SIAQVNSWLPLLKPLLASQKQAEPAALTLEEVSNLGVRELQQVCESTQAKWQSQGEQI
                     LNDTGLTLADWQPIYKALSQNQEPVLTPEQQKGLVDKGIVKMRLTFATGL"
     gene            complement(16846..18147)
                     /locus_tag="ACHK74_002828"
     CDS             complement(16846..18147)
                     /locus_tag="ACHK74_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221615.1"
                     /translation="METRGTDSSTTRQYNDPVNSEAALNLCLQLWQQGGLNANKAALL
                     LAAVPALRSLLQPIIQPKKNDAETDIVSAFSLTAPLLDAFNDLSQSGEWQLALLGLNP
                     DVRQHWINLAAARCQEAGTMSDTMVLVKLIQQLGNASEWVLAQLESTATTPQIIAGPL
                     AQTERDLLGHSLNDNAAIPALCRILRTSYTLFTVSEQNEPPAPIQAVDVTAKQLTNNW
                     CSGRLLALPNTLLDEHNLKPNADWLLVSRSGHDNMPLTELFAQQPWLFLLSLIIFVQD
                     AWAAEQRGGLLLTLPAGQNAFAPGQINVAVQGIEGDEVSLGSLAEFLVLLLGELNIPL
                     YPALDANTESINRLNRVLSSFIAELLAKKIWQFTEAGRGEAGQYRIHTSFSDACYSLP
                     LAPLFGYKSQTLQRTIKQLAQNCYANKKRAANRINLQGSSL"
     gene            complement(18150..19625)
                     /locus_tag="ACHK74_002829"
     CDS             complement(18150..19625)
                     /locus_tag="ACHK74_002829"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP0221616.1"
                     /translation="MATIFKRTPEQLSERRIDPWHYQPRFERQINKIKDNLGALPLEV
                     VIDSKRGVASGATPLGANYLEKGRVKFYRTSEVEEMFLQAEEAVFINDEDDEKLKRSR
                     LVDGDVLLTITGAKFGKSAVVSAKHLPANISQHSVRFKPDPKKVDAYFLVAYLNSKTG
                     QVAIWKEAYGATRPAIDFPSVRSLAVPKVLPLAQKYIGDKVRQAEQLRAWAKRLEGMA
                     DRKIKDLFHFNLVDSLTLKPRRMKQQVLSAVSLAPEFARAADSQMTFRNSSKLSNFIS
                     KCKCGDPIKSEDRVPGPYFYYGASGPIDTHTEFNFNGKYLIIAQDGSIGCANVADGKF
                     WANNHVWVLKVKDEYDIESICRFLDKHFPCWKGVTTGSVVPKVTSENLLNILIPIDIA
                     KNREIGSKLRLAVTTAAYAKKLTASAKTLVESLIEGQLTEQQLIQAQQALEDGDSSFD
                     QAILSKLSAEGYAIEGATPLFSDVDELYSLLEEAAQAEAEE"
     gene            complement(19625..21772)
                     /locus_tag="ACHK74_002830"
     CDS             complement(19625..21772)
                     /locus_tag="ACHK74_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP0221617.1"
                     /translation="MAKEAKKQDPQQQLEALFNKLVKQHSEFTQPLVELPEHEFAKAG
                     LIQGLVEGNLTPPVMLIALTEGHWEYAADTAKFDDLAYQLLSDIEGDWPVYAVVDDGL
                     NQRILALFGADGSDGTHGADTLPGLEELRSFDRMERDPTFRWSMRVYTRLMQRFDAFH
                     ENVYRVTKDKVNDKNDIIEEVAKLLFLETFRLHHDEDLTFKDDEGNTLRFRDVFDWQY
                     VESHGDKAVAQIKAAFEEFKSHENYVVISDDGSRNPIFSKETHLRLSVAKNYQDLLEA
                     IQNLGPVKTNDGKIAKEHGTLADVSGDLLGRVFDVFLRANFESKGGLGVYLTPNPVKQ
                     AMLEIAMHDIDDDDEMRSRLANGDFRFCDPTCGSFGFGSVALSQIDKWIDFKLVLADD
                     KKESLKQKLRDCAFTGADAAPRMVMLARVNMALQGAPKAQIFYTDNSLTTNALKPNSF
                     DLICTNPPFGTPKFDKGKNGQNSKANYEANMEQVLGGFRPTKKVVDSYNAWYDHVKMK
                     WQDIGDLELDENGEPKWAGYRTDLRRTGGTDKKPFYSLQPTTAGLALGSKPDSKGNWQ
                     PVGATIDPAVLFIDRCLQLLKPGGRLLIVLPDGILCNSGDRYVREYIMGKKDEKTGEF
                     VGGKAIVKAVISLPSDCFKLSGTGAKTSILYLQKRHANPNQPEQFLPEPQTDVFMAVA
                     ETLGYVVKNNIEDYNAGVANDLDKIVSAYKRGE"
     gene            complement(21823..22209)
                     /locus_tag="ACHK74_002831"
     CDS             complement(21823..22209)
                     /locus_tag="ACHK74_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221618.1"
                     /translation="MTHEYQRQSLEQLITQQTATPRSAAVVFNENQTLWQTLGFSQSQ
                     VSLWLASLPQSNHLKGPTTATYQVIPDIAAHLVSLLHQAGGRMPLAQVLKKLPAGITT
                     SEQQIRKLAQKHAQIEIKGPLLVLIN"
     gene            complement(22206..23549)
                     /locus_tag="ACHK74_002832"
     CDS             complement(22206..23549)
                     /locus_tag="ACHK74_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HFP0221619.1"
                     /translation="MGEVISVFEYDLLGSGKAASVGAKPVPPQVFNYLEALSLASNQG
                     SQFLKLTSRSGFKLLQVQNYAGMLSTPHGFQLEILPKVGKNLTAANARQTLLTMLSHL
                     PGFRHIQTQQATLQAQRMPLLEIFINQFLHSVSQLLKQGLRSDYVSKQGNLAFMKGKL
                     MLSAQLRHNALNRHKFFVDYDDYMPDCAANRLLHSALDKLLSLKLSSENQRWLYELRF
                     AFDGIPLSRDIERDINNLRLERGMAHYNEPMAWAQLILRGMSPSALQGNTKAISLLFP
                     MEAVFESFVAQTLPDELPPHLKVLPQAATYSLVKHGLKDCFKLRPDLLIQSHKPVQTE
                     MVMDTKWKLVNSSQQTKSLYGLAQADFYQMFAYGQKYLGGNGEMYLIYPAHDDFSQPI
                     AQHFAFSETLKLWVVPYRIMAKRGERMMWPNDVSCTMSPKLDRMAASVSYSGGMR"
     gene            complement(23542..25617)
                     /locus_tag="ACHK74_002833"
     CDS             complement(23542..25617)
                     /locus_tag="ACHK74_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0221620.1"
                     /translation="MTLVDSVEAGRLTISELIDALAKDKNYTASRWYQRYRAFTTLLQ
                     QTSTFAEPATDGLVKQLWYERDNGIASIRQGVPSLAEYQQSLPLLRELTERIRQQPDE
                     ETYQYVGNALQQAKENGLLKRMYWSLRNRVFAAFSPENYTSTVDENAFNKAAEFLNQH
                     FHLGLVLTGNWLQKNYELKQAIHAQSPDTDPYYVNMAIWHLYELLRERDNEQKQEKVA
                     STTSITRSEPIENKIILHSPTNVIFFGPPGTGKTFRLQQKMKEYTSHAVPADRDAWLD
                     SRLESLNWMQVITLVLLDLGKRAKVRQIIEHMWFQRKALLNGRNGNLSNTAWAALQSY
                     TVPESLTVDYKNRREPAVFNKTDNSEWFLVDSQLEQVEDLVELYAELKRGPKSAEAIQ
                     RFAVVTFHQSYGYEEFIEGIRARSDESGNISYPIEPGIFMRLCQRANADPGHRYAIFI
                     DEINRGNISKIFGELISLIEVDKRAGMPNAMSLQLAYSGDHFSVPGNVDIIGAMNTAD
                     RSLALMDTALRRRFDFVEMMPDLSLLSEAKVKGIELESLLEKLNSRIEALYDREHTLG
                     HAFFMPVKNALDAGDEEAAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQKQDD
                     NLQFVIEKTDDLDTLFGNNHGLRRHDQQSTAYELKDFDQEIWNIPQAYRSIYQPQQTP
                     LDEQAVNHG"
     gene            complement(25787..26659)
                     /locus_tag="ACHK74_002834"
     CDS             complement(25787..26659)
                     /locus_tag="ACHK74_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFP0221621.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQQGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRRNYPY
                     EGFVEWHRKEVFRG"
     gene            26759..26863
                     /locus_tag="ACHK74_002835"
                     /pseudo
     CDS             26759..26863
                     /locus_tag="ACHK74_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(26871..27851)
                     /gene="nanS"
                     /locus_tag="ACHK74_002836"
     CDS             complement(26871..27851)
                     /gene="nanS"
                     /locus_tag="ACHK74_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP0221622.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGVISDRFVEAILQFWREK"
     gene            complement(27916..29022)
                     /gene="nanM"
                     /locus_tag="ACHK74_002837"
     CDS             complement(27916..29022)
                     /gene="nanM"
                     /locus_tag="ACHK74_002837"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFP0221623.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSATIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            complement(29042..29758)
                     /gene="nanC"
                     /locus_tag="ACHK74_002838"
     CDS             complement(29042..29758)
                     /gene="nanC"
                     /locus_tag="ACHK74_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFP0221624.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            31213..31815
                     /gene="fimB"
                     /locus_tag="ACHK74_002839"
     CDS             31213..31815
                     /gene="fimB"
                     /locus_tag="ACHK74_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFP0221625.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT         8017 a         7723 c         7368 g         8757 t
ORIGIN      
        1 ttatttttat taattcgccg ggaatgaata agaaaaaacg atttgtattt cactataaaa
       61 attacgaatt gctttaagtg gctttaaatg actttaagtg gagatgaaca aaaaacacac
      121 aagcattgta agactgccgg aacaaatccc ccctgttgcg tctgctgaaa atattcacaa
      181 aataaagcgc tacccgaagc cggacggact tatccggtgc tgtatggaca ttaacgaggt
      241 agcccgatgc aagctgtttt ttcttccccg tctcccgccc ctgtgacgcc actgatgccg
      301 ctgccggaca tcacgcagga gcgtttttta cgactgccgg aagtgatgca cctgtgcggc
      361 ctgtcacgct cgaccatcta tgaactcatc cgtaaggggg aatttccgcc gcaggtgagt
      421 cttggcggta aaaatgtggc ctggctgcac tctgaagtca ccgcatggat ggccgggcgc
      481 attgccggac gcaaacgggg gtacgacgca tgatgatgcc tgttctgcaa aaagtccctt
      541 tttctggctt gcctttttcc ggcatttgcg gatatagttt ttccgctgtc gcaaaatcgg
      601 cagccgggcg taggaacccg tgtaactcga aggcgacata tgacgcgcca tgcgtctttt
      661 tttgtgtcgc aatcaacgcc acagagcgcc agattatggt gtggcgtgtg gtttgccgtg
      721 caggtatgat cctgttcgca atcgcatgtt atgccactga gtcaatggta gctcaggcgg
      781 ggcagcctcc gggctggccg gtattcttcg aggccggtat tcctaccccc gtctgggcta
      841 tcgccatcga gcgtaggaac tccggcgata gcagttattt gctactcgaa ggagacggcc
      901 ttatggctac aacccttacc ccctcacacc ctgaatttgt ctttgtgttt gcggctgtcc
      961 gtcgcgcaga ccgtcatccc cgtatctgca tgcttcgcac cgtcgccggt gatgaacgca
     1021 gcgcccgccg ttcccttgtc cgtgactatg tgctctccct tgctgcccgt ctgccggtgg
     1081 tggaggtgtc ccgtgcgtaa taaaaaagcc cctcagaccg tctcagcgcg tcatgacgcc
     1141 cgtgaacacc tcagcattga ggcttaccat aagctcaacc gcgccagcgc cgtatcccag
     1201 tttgttgggg gtgatttgat tcaccgtgaa ctctccggcc tgcatcagct ctacattccg
     1261 catattttca gttacctgaa tgaagatatt gattttgtgc tgaatgagct gaaagccaaa
     1321 ggcctgtgcc gcgattttct cgcccagcag aaagaccggg gagacaggac gcatgtttga
     1381 ttttccccag cccggtgaga tttaccgttc tgccggtttt cccgatgtgg ccgtggtcgg
     1441 cattctggaa gacggtattc cgtgggaaat gccgtaccgc tgcccggaca ttgtctggaa
     1501 cccgtaccgc cgtaaattca gtatccttgt gcgtatcctc gctgacgggc gcaccacaga
     1561 catcccgctg gggcgttttc tgcgggaatt tacctgtgac cgtcctgacc tgtttaaacg
     1621 cagccccgta aaccggcatg cggtactgaa agaaatggcc ggagacccgg aattacagaa
     1681 atggcgggag aaatatctgg atatttaccc gcaggacact gttccggcca gccgggcggc
     1741 accggtggcg cgggaatggc gggaaattcc ccgcacggag cctgacccgg aaatcacccc
     1801 ggataacagt taccgcaatt atctgtaatt aaaaaacgac acccgaaaaa ttaaatgtgc
     1861 gtattcgcgc agggatacgc acgtcttcag gagacgcaga tatgccttat cagttaatgc
     1921 aaccggcacg gaatgcagtc atctgtcaca gggaggaaaa caaatgaaaa cacccttacc
     1981 gcccgtctta cgcgctgccc tttaccgtcg cgctgtcgcc tgtgcctggc tgaccgtgtg
     2041 cgaacgtcag caccgctacc cgcatctcac ccttgaatca ctggaggcgg ccatcgccgc
     2101 tgagctggaa ggcttttatc tgcgccagca cggtgaggaa aaagggcgtc agatagcctg
     2161 tgccctgctg gaagatttaa tggaatccgg ccccctgaag gccgcgccgt cgctgtcctt
     2221 tctcgggctg gttgtgatgg atgaactctg tgcccgtcac ataaaagcgc cggtactgca
     2281 ctgaaggaga acaacaccat gaaaatgaac gtaaccgcca ccgtcagcca tgcgctcggt
     2341 cactggccgc gtattctccc ggcgctgggg attcaggtgc tgaagaaccg tcatcagccc
     2401 tgtccggtct gtggcgggag tgaccgcttc cgttttgatg acagggaggg gcgcggcacc
     2461 tggtactgca atcagtgtgg tgccggtgac ggcctgaaac tggttgaaaa ggtgtttggt
     2521 gtttccccgt ccgacgcggt cgcaaaggtg gctgccgtga ccggcagtct gcccccggct
     2581 gacccggcag tgacggccgc cgccgttgct gaaacagacg ccgcccggaa gaacgctgcc
     2641 gcactggcac aaaccctgat ggcaaaaacc cgtcccggaa ccggtaacgc ctacctgacc
     2701 cgcaagggct ttcccgaccg ggaatgccgg atgctgaccg gcacacacag agccggtggc
     2761 gtgagctggc gcgccggtga ccttgtggtg ccactgtatg acgacagcgg cgaactggtt
     2821 aaccttcagt taatcagtgc tgacggccgt aagcgcaccc tgaaaggcgg acaggtcagg
     2881 ggcacctgtc acacccttga aggacagaat caggccggaa aacgtctgtg gatagcggag
     2941 ggatacgcga ccgcacttac cgtacatcac ctgaccggtg aaacggtgat ggtggcgctt
     3001 tcttccgtga acctcctttc tctggccagc cttgcccggc agaagcatcc ggcctgtcag
     3061 attgtccttg ccgcagaccg tgacctcagc ggtgacggcc agaaaaaagc cgccgcagcc
     3121 gcagatgcgt gtgaaggtgt tgttgccctg ccgccggtct tcggtgactg gaatgatgcc
     3181 ttcacgcagt acggcgggga agccacccgt aaggccattt atgatgccat ccggccaccg
     3241 gctgaaagcc cgttcgacac catgagcgaa gcggagtttt ccgccatgag taccagcgaa
     3301 aaggccatgc gtatctatga gcattacggc gaggcgctcg cggttgatgc caacggccag
     3361 cttctgtccc gttatgaaaa tggtgtctgg aaggtgctgc caccacagga ctttgcccgg
     3421 gatgtggccg ggctgtttca gcggctgcgc gcgccgttct cctccgggaa ggtggcctcc
     3481 gtggtggaca ccctgaagct gattattccg cagcaggaag ccccctcccg ccgcctgatt
     3541 ggctttcgta acggcgtgct cgacacgcag aacggcacgt tccacccgca cagtccgtca
     3601 cactggatgc gcaccctgtg cgatgtggat ttcaccccgc cggtggacgg tgaaacgctg
     3661 gagacccacg cccccgcgtt ctggcgctgg cttgaccgtg ccgccggtgg ccgtgcggaa
     3721 aaacgcgacg tgattctggc cgcactgttt atggtgctgg cgaaccgcta cgactggcag
     3781 ctctttctgg aggtgaccgg tcccggcggc agcggcaaaa gtatcatggc cgaaatagcc
     3841 accctgctgg ccggggagga taacgccacg tcggccacca tcgagacgct ggaatccccg
     3901 cgtgaacgtg ccgcgttaac tggcttctca ctgatacgcc tgccggacca ggaaaaatgg
     3961 agcggcgacg gtgccggact caaggccatc accggcggcg atgcggtgtc cgtggacccg
     4021 aaataccggg atgcgtactc cacgcatatc ccggcggtga ttctggccgt gaacaataac
     4081 ccgatgcgct tcaccgaccg cagcggcggc gtgtcacgcc ggcgggtgat tattcacttc
     4141 ccggaacaga tagccccgca ggagcgcgac ccgcagctta aggacaaaat cacccgcgag
     4201 ctggcggtca tcgtgcgtca cctgatgcag aaattcagcg acccgatgct cgcccggtca
     4261 ctgcttcagt cccagcagaa ctcagacgag gcactgaaca tcaaacggga tgccgacccg
     4321 acgtttgatt ttatcggcta tctggaaacc ctgccgcaga ccagcggcat gtatatgggg
     4381 aacgccagta tcatcccgcg taattaccgt aaatacctct atcacgccta tctggcctac
     4441 atggaggcaa acggctaccg gaatgtactc agtctgaaaa tgttcgggct ggggctgccg
     4501 gtgatgctga aggaatacgg actgaattac gagaagcgcc ataccaaaca ggggatacag
     4561 accaacctga cgctgaaaga ggaaagctac ggcgactggc tgccaaaatg tgacgaccct
     4621 gcaacagcct gacccatctg accggcatct gccggtcttt ttttatccct gaattccccg
     4681 caggtgaaca atccactgtt cacccttcac cgtatattca cccgttatca cactgaaatt
     4741 aaaagagaaa aacgaaaggt gaacagtgtg aacaatcaaa tcaaaaaaaa cttttttctt
     4801 cctgagtgat ttcagtacgg gggattaatc accggtatga gtcacaccgg cagaatgccg
     4861 gaggtgaaga atcgaatgtt cacccttcac ccattattca ccacctatca ctatgaaata
     4921 aaaggagaaa aaagaaaggt gaacagtgtg aacagttctt ccgaaaaaaa attttttatg
     4981 tggataaaag atgcactggt ttggatccga actacggatc caaacttact gattaaaaat
     5041 tatttaactt tccgatttgt tgatgggctg aaaatagatt ggtcagaaaa acagtggggg
     5101 cacaaaaggg ggcatatttc attgttgtgt tttattattt gttttttatc attgacttat
     5161 tttgatgttt gagtccggcc ttcgcaccaa aagtatgtaa atagacctca actgaggtct
     5221 ttttttatgc ctgaaatcca gtatccgcaa ggctttcctc gctttttcac tcaacctaag
     5281 tcaacctgat tcaatctaca tcaacttact gatgcgggta caactgcggg tatttcccgg
     5341 ttcgattatg tttgtaccca cacagggccc ttgaaaggat actcatcatg gctctgagtg
     5401 atgtgaaggt ccgttcggct aagccggaag caaaagccta taaacttact gacggtgaag
     5461 gcatggtttt gttggttcac cctaatggct ccaaatactg gcgacttcgt tatcgttttg
     5521 gcggtaagga gaagatgctg gcgctgggga aataccctga agtctcgctg gtcgatgcca
     5581 gaggtcgccg ggatgaagcc cgtaagttgt tagctaacgg cgtcgatcct agtgagaaca
     5641 agaaagctgt taaggtagag catgagcaag aggtgataac atttgaagtg gtagccagag
     5701 agtggcacgc cagcaatcag aagtggtctg catcgcatag cgctcgtgta ttgaaaagcc
     5761 tcgaagataa tctctttgct gctatcggta agcggaacat tgctgatctg aagactcggg
     5821 atctgcttgt ccccatcaaa gcggtggagt cgtctgggcg tctcgaagtc gctgctcgtt
     5881 tgcagcagca tactaccgcg attatacgct ttgctgtaca gagtggatta atcgactaca
     5941 accccgctca agagattgct ggtgcggtag ctacggcgaa aagacagcat cgtgctgctc
     6001 tggaacttaa ccgcattcct gaattgcttc aacgcatcta tcactattct ggcaggccgt
     6061 taacccgact ggcggttgaa ctcacattgt tagttttcat ccgttcaagc gagttgcgtt
     6121 ttgctcgctg gtcagaagtt gattttgata ctgccatgtg gacgattccg ggagaacgtg
     6181 agccactgga gggagttaag cattctcagc gtggttcgaa gatgcggact ccacatcttg
     6241 tacccttatc tcgacaagct ctcaccattc tggaaaaaat caaaagcatg agtggaaacc
     6301 gtgagctgat cttcgttggt gatcacgatc cgcgtaagcc aatgagtgag aatacgctta
     6361 acaaggcctt gcgagtgatg ggctatgaca cgaaaacgga agtgtgtgga catggtttca
     6421 ggacgatggc gtgtagttct ttgattgaat cgggattgtg gtcgagggat gcagtagagc
     6481 ggcagatgag tcatcaggaa cgtagctcag tacgggcggc ttatatccat aaggcggaac
     6541 atcttgggga gcgaatgttg atgctgcagt ggtgggctga ttatctggat gcgaatagaa
     6601 tggcggctat cactccattt gactatgcaa agattaatag ttcttttcga taattctagc
     6661 taagtatatg aatattaact ttttttaatt aaaggcttat aactgagaag cctccgccct
     6721 aatggggaga ggctttgtct taactcaatc aagaaggaaa tcgttatgta aaactttatt
     6781 tttttgtccg gtatgtgaac ggaagtgtta aatatccact atgccttgtt cgcgcaatgg
     6841 tcatcctttc aatcaaataa ctccaagctt tgcatacaat tcttttttct ctttcatgct
     6901 gttatccaat ggtgctactc gcaatgccgc tttcaaataa gccgctgcac cagagtaatc
     6961 acgcatttgc tcgcaaacta aaatgagttg gcttaacaca tggaacttca ttggagttgt
     7021 ttggtttact ttctccaata gtactcttgc ttcagaatat ttcgcttcaa ggccaaggat
     7081 agatgattct aaatacatgg tggccgcgtt ttctgcttca gcttgctttc gttgcaacag
     7141 ccagaaattt gcttgactga tattgcgtaa ctgtaggttt gccttaacta tcaactcgaa
     7201 caaagctgct ggttgagtca atttaccctc aagtaagatc gctttattat cttctacaac
     7261 tttttcaaag tcgaataagc gattggcgcg atacaaggca ataagttgct gatgtaaagc
     7321 ttgtagatct ttactgcggc tatactcctg cgacgctaat tgagcagctt tttggtaaga
     7381 ttgatcggac attgcttcaa gtaccgcact tgaccagtcc tgagcaggtt tagtttgaaa
     7441 aacgggagca atagcaccaa cttttaattg cttagtggca gcagaatcaa atgcatgaat
     7501 taagtcggaa atagtggaaa agcgatcctc cgctcgtttt tgtaaacaag taagaaccac
     7561 ttgttccagg tccgtatgaa tagagctatt aatctctgtc ggaagcttga aaggtgcgtg
     7621 gcgctgaact tgtgctggcg tatcatgggg cagctttcct gtcagcagct ggtatagcaa
     7681 aacacctaag ctatagatgt cgcccaatag tagctcttcc aggctgcctc tacggctatc
     7741 gacgttggag aactctgggg ccgaatacag cacagtcccg ccattttcac gggtacgaac
     7801 gtaaatttct tctggtaaag ctgagctgcc aaagtccgtc aacttcacca ggtcccgctc
     7861 tccaaccaaa atgttatgtg gcttaatatc tccatgagat attggtactg gtaattcggc
     7921 caaataatgc acagcacaga gcacttgacg atatattctg aacaaccgtt cataggtctg
     7981 gccaaaatta cgctgcctgt catcgagctt ttgcgccagg gtttggctgg gaaaatactc
     8041 catttcaata tagaaccatt cattatctat acggcccata tcaaaaattt gcacaatatt
     8101 ttcgtgcggc tcgacgttta aaagctcttt accttcggca agctgacggt cttcccgagt
     8161 aatatcctta gggattttta ctgcgacaat ttttccatct ttcaggcgtt ccgcccgcca
     8221 tacccagcca tggctgccat caccaaggca ttcaagtaac aggtatttgt caccaaggcg
     8281 tttattcgtc cctggcaaaa aatgtgctga ttttatattc acatcaacac ctcatagtga
     8341 attctgtcca aaacaatcat gttcaggttt ttgataaata aaaagcgact aaataccacc
     8401 tcaacatcag gagcggcgat tacctgggta cccgtggacg aagtaaaacc catacggcat
     8461 atgtctttca gtttttgagt atcaggtaca cctactaagc cctggctcca ggcactgagg
     8521 accagggttt tggggcaatg attccagcgc tgtgctgctt ccagtaagtt ttttaatttt
     8581 tcttccagct tttgctgccc cttcgctgaa ccatcaaagc gttcaaattc gattaaacac
     8641 acaggcgatg cagaagattt atgaaaccag cccgagtcag agcgaatatc agcaccaaat
     8701 gtccctggag ccgggcattc cactaccgcc ataaatgact gttcatgccc gaatgtattc
     8761 caaacgctac agcccaggct gtggaccaat acatcctggt ccaatagctt accgcttgct
     8821 aaaagtggaa atgtctcatt tacccaggca acttcaggca taggcacctt agccactgaa
     8881 agagccccaa acacgcagta cctctggtga ttcaccaccg tgaattaatg ccttactacc
     8941 aactttaaca agcagtggtg cagggattga acgaccacac accaaagctt caccggttga
     9001 taaactcggg agttcctcca gatcagcttt gctcaccata tctgatgctt tagctataaa
     9061 tcgttgatcg tcagggttct ttaagcgcat cgtaattagc gtattgcact gagaggtcac
     9121 atctgaatct aatttcgatg gacgctggct cacaatgcca aatccaacac caaatttacg
     9181 accttcacca gcgatctttt taataatgcg atgactgact gcgttgccgc cggccggggc
     9241 aaaattatga ccctcttcgt aaatgataaa gcatgggcgc agcgggtccg ttttgctcga
     9301 ggctgctttt aatatttcac tagataacag tgcacaaatg acttgcttag cggtatcact
     9361 caaaccttgt aagtcaacaa cgactaaacg cccctgttga ttgctcgggc gacccaccat
     9421 tttgtaaata tcagttggct cagccattgt tgcgctgtaa aaacttttag cttcattaag
     9481 cacacgtgaa agcttcatcg aggcgacggc ggcactacgg cctgataacg ccttcgcctc
     9541 agcttcagaa aggtcgtccc actcttttaa ctcttcaata ccatcaccta ataagaagcg
     9601 taggcggtta atatctcgtg gctcagtctt atctgcatag cgccaataac gaattgcaac
     9661 atcgagtact cgttgctgcg gttccgttaa gccaggtaat atttcagcaa tatcgtccat
     9721 ttcaaaatgg tcaaactgaa gcgctaatgg cgtattctta ctggcgtatt tatgtttaaa
     9781 agattcatgc tgcggcgagt aaacctgaat acctgcaccg gcggcttgca gcttttcaaa
     9841 ggtctgtttt atcagtggca gtgcttcttg atcacgctta tcgtcagtgg cttctaagaa
     9901 gcttgagaat tgcagattac cgccagcgag ggctttaccg tattcaccat gtgggtcaaa
     9961 tactactacc gtaccgttat taaccgcaac taaacgctca ataatgcggc caactgtata
    10021 agacttacct gaacccgtca ttgccagaac agccaaatgc tcagtgacca acttgttcac
    10081 gtcaataaaa acaggaactg tattctcacc tttgtcgtag cctactaagt tacctaagtg
    10141 caaactggag tgttcattaa attcgtaaaa atcactcaaa aactgaaaat cgacggtttc
    10201 aacagcggta cccgggttaa gcgggcgacg tggaattttg atttgacgag taacaggatc
    10261 gcggtaacca accagctcga cctgagcaaa catattctca ccagttacat tcgcaccggg
    10321 caaaagctca agctcggtta caccatcgcc aaagccggcg ttatagagca tattagtacg
    10381 gcttatcttg gtcactcgcc ccagtacggc gacttgcccc tcatctttgc gttcctggtg
    10441 cataatgcgt acaaactcac cacggcgtac tgaaaacgag tcttgtacaa ccatagttaa
    10501 ctgactggca tcaccagtgt taccgactaa tttacctaaa actttataag ccattattca
    10561 atccctcatc gaggcctgcc aaccaccctg cctcaatatc attattcttt agtgcctggt
    10621 gtaaaccctg gcgataaaac gtagcaaatt taggtaaaat accaagctgt tgataaccaa
    10681 gtaattgtgg caacggcatt gcattacctt tgttttgcat atccagcacc atcaggcgag
    10741 aggcaactaa atcgagatca gcacttttcc attctggttc gtcacccgct aattgcacca
    10801 tttttatctg tccacctcgg gcactacgga agtgaaaacc aattagacct tgcttagctt
    10861 caatattggg ttcaatgcgc ttttcttgtt cagacaaacg aaatgcgatg gtgcgcgaat
    10921 agttgcctaa aatgccattg ataaatcgca aatctcccac gccaaccaaa ctttcatcca
    10981 gtccttccag attttgcatc aactctttag aaaacccatc agatgctaaa atttgtttgc
    11041 gtccttgttc ggtacgtaaa tcttgtttgg cgatacttac aatggcacta aagcgctcag
    11101 ccaaaatgct cgcaagtact acaccatttt gattccaggg aaaaattctg gagcgatggt
    11161 tttgccaaaa agtttctatc acttgtctgg ttttttcgta ctcttgttta tgacggacat
    11221 ttctctccag cggagccata tcccgtgcta aaaacaacgg cgtatccagc aaaattaaat
    11281 cgccaggctc ataacctaaa agttttttct caactaactg ataagcctga cgaatttcaa
    11341 ttaaatcaag ccgtttacgc tgagcttcaa aatcaatatc atccagttct gataccgagt
    11401 cgctacccac cgtgctgact ttattgtcat ctattaacaa atctgtgcgc actgctgcac
    11461 tggcataaat aaaaccaccc aataccttgt ctaagcacga tttagtcgca ataccggcta
    11521 cgcgtaaatt aacaattgtg ggcttgggga ccgctttcac taagccagca tcacggaata
    11581 gcggcgccac ttgctcaata tcacggacct gtttaagcaa gcgtcctgca cttcccggta
    11641 agcgaagctg gctatcgatg gcgagcgatt gcgctaaatt catcgttttg ttctcattca
    11701 tgcttatagc gttcctgtgt gtagctgctc gccaagtaat ggggcataag cccaatattg
    11761 cgaggcttgt aggcatgttt taccggtagc tgttatgccc gcgtcgtcga taaaatcgtg
    11821 ctgccgcaac atctcacccc gcttgacaat atcaaggcta ttaacatcaa acatcagctt
    11881 aaccgcatta tgctcagaca acgcttgtaa gttcatgcca ccgccagcgt agagtaatag
    11941 caatacaacc agatcgtgct tatctggcgt gtagtcagtt gatggcgctt taggttcgat
    12001 tattcgttca gcaatgcctg catggtccac aaaccaattc aaccagctca ccaggagctc
    12061 actatgatcg agtaaagtca gcgaccacaa aggtaacgtg gtaaaggcta atagcccact
    12121 gttgctgtgt tttcgtatgt agcgggtatg cactacatca ccggcctggt gctggtagcg
    12181 atctgcgctg ccagtaaagc cagcaatagc ctggtctgtt tcactggcta gcaaagcggt
    12241 tagccccggt tcctgccccc tcaagctcgc tggtactaac tgccaatcca gtggataggg
    12301 caattgcgct aataaggtaa attgttctgc tttcaggttt attaccaaag cggcacaacc
    12361 tgcctgcata gcccagtgat acaggtgctg catagtatta gcgtcagtca actcttctgc
    12421 ttgcactatt aacaaaccgc tggctggcgg cgagctaatc caatccttca ttaagggggt
    12481 agcccccgca acagtttgca aaaaacggcc tttacgatta ctggccagtg ccgcagataa
    12541 ataacgactc atcgcgtcac ctctacaaac cagtggcaaa ggtaaggcgc atttttacaa
    12601 tgcctttgtc tacaagtcct ttctgttgct caggtgttaa cacgggttcc tgattttggc
    12661 tcaaagcctt ataaatcggt tgccaatccg ccagtgttaa gcctgtatca tttaatattt
    12721 gctcaccttg actctgccat ttggcctggg tgctttcaca cacctgttga agttctctga
    12781 cacccaaatt gctcacttcc tccagagtca acgctgctgg ttccgcttgc ttttgagaag
    12841 ccaatagcgg ttttaataac ggcagccagc tgtttacctg tgcgatggag ttgcgtctta
    12901 cttggtttag acgctcttca attttaagta gctggccttg aatcggtgta agttgaccgc
    12961 gtgccggttt taaaagctct tctggtaaat cccgtaacga tgaaaactgc ccattgcgca
    13021 aactgttttg catactttga cgcttgctct cggcatcgtt ggggagatcg gcaatagcat
    13081 cttcaatgag ctgtaatttc attaccagtt ttggtagctc tgcaatgtca tcggcgaact
    13141 gatactcagc cgtggcgcta ctaagccatt cttgcagttg ttgtgcacgt agtttttgct
    13201 ctgtgaggtt attaaccagc ttactaaatc gttctttgca gttgcggtag ctggacaaaa
    13261 tatgcccctg aatctccgct atcggcaact gttgatcatt gtgcaggttt aaacccactt
    13321 cttgcgctaa ggctgaaagc ttgtcatgcg ctctacctaa atcgagttta cgcaggtaat
    13381 gcagtaaggt atcgctatct gcttgctgct cttttcggcc agtggcactg ctggtatcgt
    13441 cttttaacgc tccatctaaa atatgaccta tggtgcgcag cgtgttggtg tacagacgtt
    13501 tcggaaaatt cgtcagcggg gcacactctg tttcaacatc tatgattaaa tcaacgagtc
    13561 ggttgttgat acgctcagcg ctgcactcaa caaactcggc aaataaacag gcctgttcaa
    13621 tccgttgata aagcgaggtc gttttgtctt ctaagctgag aatatgggca tcttttagca
    13681 taggcctgga actattaagc ggtttaacct ttgctaccag ctccagcact tcagcgcgtt
    13741 tgtggataag ccctgccaat actttcgtgt cgttggcctc gaccatatca gccagttgtt
    13801 cttcttgctg tttgagcacg ttaaatagcg attgtgcttt ttccagctgc tctttagctt
    13861 ccacggtgac gaaacccagc ttatttttac ccagaggcgc gaacatttca ttaatgcgtg
    13921 cataaccata cacgcggctc atgacttcca cattggctgc atagctgccc atatcgttac
    13981 cgcggaacca gttttgcgct tctctaatgg cattttctaa gcgggagcgc gggtagtgct
    14041 cccaaattgc ctgtttttct gttggattgg ttaaagtgag taagttcagc tctccacacc
    14101 accacatcca gtcattaaac acgcctttgg cggtaacact cgtttcagcc acataggcga
    14161 aataaaactg ttgcttaaac ccttcgaatt gccacttatg ctttttatta gggtgagcaa
    14221 tacgggctaa aaataatggg atctgcgcct ggcttcgctg cacattatta agttcagtaa
    14281 atagcccggc atgttcatcg gccgtgtagc ctttggcaat atagctgccc ggcaccttaa
    14341 gtttatccaa caagctgctc agctcattca ctggcacgcc gtgttgctgc tgtaaatcga
    14401 gaatgccatt taactcaggg tgagcaatgg ccagctgata ccagccttta aacaacaggt
    14461 ctcggtcatt cgatgataat ttaccatcta cctttaacgg ccaggcaatt aaaccgcgct
    14521 gctgcaaacg ctggcgccac ttgtgaatgg cgtccgtggc aaaggttgtc aaggcatgca
    14581 gcttgttttt gaatttcgcg gtaaagctgt cggcagttaa attcacgccg tgcaactgac
    14641 atgctgccaa acttaagcca atacgctcta actggtctgc ttcgcttggg ttgacataat
    14701 gcagcagtat cgcatcacgc aactcatttc tttcatccag tcggctgtat tgctccatga
    14761 gatgtgcaga agcagttaac gctaatactg gcgtgcgacc attttggtgg ctgtggtttg
    14821 ccgcaaaggt gtgaagattg ttcagctcaa ttaggttgct gacccaggca aacaaggctt
    14881 taccttctgg gtgaagctta aatccatcca gggtaagtaa accaccctgg cggccacggg
    14941 gctcggttaa aatggccaaa ggtgcatcgt cgctgcccgc tttattactc agcaagtttt
    15001 catcataggt ccagttgtta tcaagtaatc tcacaaagcc cgttacacgg gctaatgcct
    15061 tgatgagcgg cttctggcta ctttgtttgc taagttcact cattgcctgt tcatgagcgt
    15121 cgcccatggc gggatcttta aaaatcagtg agttttgctg ggcactgcga tagcgcaaat
    15181 ccaggcgcag taacatcgcc acactggggc caatatgcgt tgcttcgctg gcgtccagtt
    15241 cggtagtttc acccactagc ttgtgccata aggcatcggc agcgaattca atcgctgggt
    15301 ggtcatacag caagctgagt aagtatttaa actcaccttt tgacagaggt agtaataccg
    15361 cgcctttatc gccgcttgcg gcttgttctt taattgaaaa agtacgcagg ttatccagca
    15421 aagtatctaa gttcagcgct tgatctactg cagggtagta ccattcatct ttatcgcggc
    15481 ggaatttggc ttgctccagg gcttgtcggc actgtaggcg gtcaatgctc agtttgcggt
    15541 aatgagcggc tatgggttcc agatcttcgt ttttcagcgc cagcaattct gtggcatagg
    15601 ccatttcagc taagcgcacg ggtaattggc cgtagagcaa actacgacat tcggtcaata
    15661 gtgcctggtc gcgagtttga atgccttcta tcgcgccttt atccagcaca tgcctggcta
    15721 cgcggccaga gcctgccagc acagcttcaa ataaatcgcc cacatccttg atactgcggc
    15781 ctgcttgttg gtattggctc agcactgcat ccacattagc catgaccacg ttaaaccaac
    15841 cgaagttggc accactcatg gcgtatgcag cttccactaa accagcaggg taattaaaac
    15901 ccagcttgcc gtcttttaag aggcttgcca catagtctga tacatcggca aaggcattat
    15961 gctcaagttc cactaactca aagcggcgtg cgaccgattg aatttcgcgc aattgctggc
    16021 caagcagtgg tgaacacagc gctacatagc gcaaccatgg aagtttacgg cgagggtctt
    16081 cctctttaat tgctttacca atcaggcgaa tggcctggcc atcgaggcgt ttattttgtt
    16141 catcttccag gccaaaggtg gcggcttctg caacggtttc cagctcatcc agcaccacca
    16201 acatatactg cacgccaaag ctctttaata ctttataggc ggcttgcacc aaactgacta
    16261 ccagcgtttg gtcagtatag aggtcttctt cgctgtggtt ggcgttcagc tgtaaggcat
    16321 ctgctaacag ttcagattta aagccaaatg gcattaagcg ttcataggct tgtttggcaa
    16381 tttcactttg aatcgatcca tcaaaggttt gggtggccag tggtaatagc gcctgatata
    16441 aaccaaaagc aaaccagtta tccgagtttt gataatctga tgccacttgc gagtagcgaa
    16501 tgtagagcgc caggtatttg tcttgcgtgt cgcgattaaa cagctgctta tcgtgcagtt
    16561 ggccttgttc gtcgcggaca aaccagcccc ttgaacagtc gttaatttgt gcgaccaatt
    16621 catggcctaa acgtgattta ccccgacccc attcggcttc gacggcaaac acatgggcaa
    16681 agcgatcatc gtcgtgatcc accgtatgca aaaaggtttg aaagcgttta aaaaagcggc
    16741 tctggcccac taacgggtcg cgaggtggat aatctgatac gcccgtcgcc gcccagcgat
    16801 gttgttttgc caggctatca accatctgtt cgtttaattc aaaactcata acgacgatcc
    16861 ttgaaggttt attctattgg cggcgcgttt tttattggca tagcagtttt gcgccagttg
    16921 cttaatggtc cgttgtaacg tttggctttt gtagccaaat agtggtgcta acggcaggct
    16981 ataacaggca tcgctgaagc ttgtatgaat tcgatattgg ccggcctctc cacgacccgc
    17041 ctcggtaaat tgccagattt tttttgccaa caattcggca atgaatgagc tcaggacgcg
    17101 gtttaggcgg ttgatgcttt ccgtgttcgc atcgagtgcc gggtagaggg gtatattgag
    17161 ctcaccgagc agaagcacaa gaaactcggc caaactaccc agactcacct catcaccttc
    17221 aatgccctgt accgccacat ttatttgacc gggggcaaag gcattttgcc cggcaggtaa
    17281 ggtgagcaac aaaccgccac gttgctcggc agcccaggca tcttgcacaa agataattag
    17341 actgagtaaa aataaccagg gctgttgggc gaatagctcg gtaagcggca tgttgtcatg
    17401 acccgagcgg gacaccaata accaatcggc attgggttta aggttatgtt catcaagcaa
    17461 ggtattaggg agtgctaaca accgcccact acaccagtta tttgtcagtt gttttgcggt
    17521 gacatccacc gcttgtatag gtgccggtgg ttcattttgt tcgctcacgg taaacaaggt
    17581 atagctggtg cgcaaaatac gacatagcgc gggaatggca gcattgtcgt ttaggctatg
    17641 accgagcaaa tcgcgttcgg tttgtgccag agggccggca atgatttgcg gcgtggtggc
    17701 agtgctttcc agctgggcta agacccattc actggcattg cccagttgct gaatgagttt
    17761 aaccaatacc attgtgtcac tcatcgtgcc tgcttcctgg cagcgagcgg cggctaaatt
    17821 gatccaatgt tggcgaacat ctgggtttaa gcccaacaac gctaattgcc attcacctga
    17881 ctggcttaaa tcgttaaagg catcaagcaa tggtgcagtg aggctaaagg cgctaacgat
    17941 atccgtttca gcgtcgttct ttttcggctg tattattggt tgtagcagtg acctaagggc
    18001 aggcacagcc gccaataaca atgctgcttt gttggcgttc aaaccacctt gttgccaaag
    18061 ctgcaggcag aggttaagtg ccgcttcgct atttacgggg tcgttatatt gcctggttgt
    18121 actgctgtcc gtgccccttg tctccatctt tactcttcag cctccgcttg cgccgcctct
    18181 tccagcaaac tgtataactc atccacatcg ctaaatagcg gtgtggcacc ttcaatggca
    18241 taaccctcag cgctcagttt gcttaaaatc gcttgatcaa agctgctgtc gccatcttcc
    18301 agggcttgtt gggcctggat tagttgttgc tcagtcagtt ggccttcgat taatgactca
    18361 actagagttt tggcagatgc tgttaacttt tttgcatatg ctgccgtagt aacggctagt
    18421 ctcagcttac ttcctatttc tctattcttc gctatgtcaa tcggaatcag aatatttaac
    18481 aggttttctg aagtcacttt tggtacaacg ctgccagtag tcacaccttt ccaacacgga
    18541 aagtgtttat ccaggaacct acaaatagac tcaatatcat attcatcttt tacctttaaa
    18601 acccaaacat ggttgtttgc ccaaaacttt ccatcagcta catttgcaca gccaatagag
    18661 ccatcttgcg caataatcag atatttaccg ttgaaattga attcggtatg agtatctatt
    18721 ggacctgatg cgccatagta aaaatatggt ccgggtactc tgtcttccga tttaataggg
    18781 tcgccacatt tgcatttact aatgaaatta gatagttttg atgaattcct aaaggtcatt
    18841 tgactatcgg ccgcacgtgc aaactcagga gctaaagaca ctgctgataa tacctgttgt
    18901 ttcattcttc taggtttcaa tgttaatgaa tcaacaaggt tgaagtgaaa taaatcttta
    18961 atttttctat cagccattcc ttcaagtcgt ttcgcccaag cgcgtaactg ctcggcttgg
    19021 cgaactttat ccccgatgta tttttgggct agaggtaaaa ctttaggaac cgctagcgag
    19081 cggacacttg ggaagtcaat tgcagggcga gttgcgccat atgcttcctt ccagatcgct
    19141 acttgtccgg ttttgctatt caaatatgcc accaagaaat aggcgtcaac ttttttggga
    19201 tcgggcttga atctgactga gtgctgacta atgttggccg gtagatgttt agctgacaca
    19261 acagccgatt taccaaattt tgcaccagta atcgtaagta aaacgtcgcc atccactaat
    19321 cggctgcgct ttaatttttc atcatcttca tcgttaataa aaacagcttc ttcagcttgc
    19381 aagaacattt cttcaacttc agaagtgcga taaaacttaa cacggccttt ttctaaataa
    19441 tttgctccta aaggtgttgc gccactagcc accccgcgct ttgaatcaat taccacttct
    19501 aggggcaaag cacctaggtt gtcttttatc ttattaattt gtctttcaaa tcgcggttga
    19561 tagtgccaag gatcaatccg cctttcagac aattgttcgg gtgttctttt aaaaatagtt
    19621 gccatcactc accccgctta taagccgata caatcttatc cagatcgttt gcgacaccgg
    19681 cgttatagtc ttcgatattg tttttcacca cataacccaa tgtttcagcc actgccataa
    19741 acacgtcggt ttgtggctcg ggcaaaaatt gctctggctg gttggggtta gcatggcgtt
    19801 tttgtaaata caaaatcgag gttttggccc ccgtaccgga gagtttaaag cagtcgctcg
    19861 gcaatgaaat tactgctttt acaatagctt taccgccaac aaactcgccg gttttttcgt
    19921 cttttttccc cataatgtac tcacggacat aacggtcgcc ggagttacat aaaataccat
    19981 caggcagcac tattaacagg cggccaccgg gtttaagcag ttgcaggcag cggtcgataa
    20041 aaagtactgc tgggtcaata gtggcaccta ccggttgcca gttgcccttg ctatcgggct
    20101 tgctgcccag cgcgagccct gcggttgtag gctgcaggct gtaaaacggc tttttatcag
    20161 tcccgcccgt tctgcgcaaa tcggtacggt agcccgccca tttaggctcg ccgttttcat
    20221 ccagctctaa atcaccgata tcctgccact tcattttcac gtggtcatac caggcgttgt
    20281 agctatcaac gacttttttg gtcggacgaa aaccaccgag cacttgctcc atattggctt
    20341 cgtaattggc ttttgagttt tgaccatttt tgcctttatc aaatttaggc gtaccgaacg
    20401 gcgggttggt gcaaatcagg tcaaagctat tcggcttcag agcgttagta gtgagtgagt
    20461 tgtcggtata aaaaatctgc gctttggggg caccttgcaa tgccatattc acgcgggcca
    20521 gcattaccat gcgtggtgca gcatcggcac cggtaaatgc acagtcgcgt aatttttgtt
    20581 tcaatgattc ttttttatcg tcggccagca ccagcttaaa atcgatccat ttatcaatct
    20641 ggcttaacgc caccgagcca aaaccaaagg agccacaggt ggggtcgcag aaacgaaaat
    20701 cgccattggc taagcgcgat cgcatttcat cgtcatcgtc gatgtcgtgc atagcaattt
    20761 ctagcatggc ctgttttact gggtttggtg ttaaataaac gccaaggcca cctttggatt
    20821 caaagttagc gcgtaggaat acatcaaaca ctctgcctag taaatctcct gacacatcag
    20881 ccaacgtgcc gtgttctttc gcgattttac cgtcgttggt ttttaccgga cccaggtttt
    20941 gtattgcttc cagcaaatcc tgatagtttt tcgcaacaga cagtcgcagg tgtgtttctt
    21001 tactaaagat ggggttacgt gagccatcat ctgagatcac aacgtagttt tcgtggcttt
    21061 taaattcctc aaaggcggct ttgatttggg caacggcttt atcgccgtgg ctctccacat
    21121 attgccaatc aaagacatca cgaaaacgca gggtatttcc ctcatcatct ttaaaggtaa
    21181 ggtcttcatc gtgatgcagg cggaaagttt ctaaaaacag aagcttagct acttcttcaa
    21241 taatgtcgtt cttgtcgttt accttgtctt tggtaacgcg ataaacattt tcatggaagg
    21301 catcaaatcg ctgcattaag cgtgtataaa cacgcatcga ccagcgaaag gttggatcac
    21361 gctccatgcg atcaaagcta cgtaattctt ccagaccagg cagggtatct gcaccgtggg
    21421 taccgtcaga tccatcggca ccgaacaagg ccaaaatacg ttggttcaag ccatcgtcca
    21481 caacggcata tactggccag tcaccttcga tatcacttaa cagctgatag gctaaatcat
    21541 caaactttgc agtgtcagcg gcgtattccc aatggccttc tgtcagcgca atcaacatga
    21601 ctggtggcgt taagttgcct tcaaccaggc cttgaatgag accagccttg gcgaattcat
    21661 gctcgggtaa ttcgaccaat ggctgagtaa actcggagtg ctgtttgacc agtttattga
    21721 acagtgcttc tagctgctgt tgcgggtctt gtttttttgc ttcttttgcc atgatgtttt
    21781 ctaaaatcct ttaatacagc caatggttac tacaaatgcc ggctaattta tcagtaccag
    21841 caaggggcct ttaatctcta tctgcgcgtg cttctgtgcc agtttgcgta tctgctgttc
    21901 actggtcgta ataccagcag ggagcttttt cagtacttgt gcaaggggca tgcgcccacc
    21961 agcttgatgc aacaatgata ccaaatgtgc cgcgatatca ggtataacct ggtacgttgc
    22021 cgttgtgggc ccttttagat gattactttg aggcaatgag gcaagccata gcgaaacttg
    22081 agactggcta aagccaagtg tttgccagag ggtttggttc tcattaaata caacggcggc
    22141 gcttctggga gttgctgttt gctgagttat tagttgttcc aggctttgtc gctggtattc
    22201 atgggtcatc tcatgcctcc actataggat actgaagcag ccatccgatc cagtttgggg
    22261 ctcattgtac aggacacgtc attcggccac atcatcctct caccgcgttt tgccattatc
    22321 cggtatggca caacccataa ttttaaagtc tccgagaaag caaaatgctg cgctataggt
    22381 tggctaaagt catcatgcgc agggtaaatc agatacattt cgccattccc accaagatat
    22441 ttttgtccgt aggcaaacat ttgatagaag tcagcttgtg ccagtccata gagtgatttt
    22501 gtctgctggc tgctattcac cagcttccat tttgtatcca tcaccatttc ggtttgaact
    22561 ggtttgtgtg attgaatcag caagtcgggg cgaagcttaa agcaatcttt aagtccatgc
    22621 tttaccaaag aataagttgc tgcttgaggt aaaactttga ggtgaggtgg taactcgtcg
    22681 ggtagggtct gtgccacaaa ggattcaaat accgcttcca tagggaacaa aagtgatatc
    22741 gctttggtgt ttccctgcaa agcactcggg ctcattcccc tcaggatcaa ttgcgcccat
    22801 gccattggct cgttgtaatg agccatacca cgctcgaggc gtaagttgtt tatatcacgt
    22861 tcaatatccc ggctaagtgg aataccgtca aacgcaaagc gcagttcgta aagccagcgt
    22921 tgattctctg atgacagctt tagactaaga agcttatcta gcgcggagtg taagagccga
    22981 ttggctgcac aatcaggcat gtagtcatca taatcgacaa aaaacttatg gcgattcagc
    23041 gcgttatgtc gcagctgtgc agaaagcatc agcttgccct tcataaaagc cagattacct
    23101 tgcttgctca catagtcgga gcgtaaaccc tgtttaagca attggctgac gctatgcaaa
    23161 aactggttaa taaaaatctc aagcaaaggc atacgctgag cctgaagagt ggcttgctgg
    23221 gtttgaatat gtcgaaaccc aggcagatgg cttagcatcg tcagcaatgt ttgacgtgca
    23281 ttagccgctg tgaggttttt accaaccttg ggcagtatct caagctggaa accgtgaggt
    23341 gttgaaagca tccctgcgta gttttgaacc tgaagcaatt tgaagcctga gcgagaggtg
    23401 agtttgagga actggctccc ttgattactt gccaggctaa gcgcttctaa ataattaaag
    23461 acctgtggag gcaccggttt tgcgccaact gaagccgctt ttccactccc caagaggtca
    23521 tattcaaaaa cggagataac ttcacccatg atttactgcc tgctcatcaa ggggagtctg
    23581 ttggggctga taaattgaac gataagcctg tggaatattc cagatctctt gatcgaaatc
    23641 tttgagctca taagctgttg attgctgatc atggcgtcgt aaaccatggt tgttaccaaa
    23701 aagcgtatcg agatcgtcgg ttttctcaat cacgaattgc aggttgtcgt cttgcttttg
    23761 attgtctgcc agcaccaacc ggatcttgtt ccagtcatcg aaaaagtatt cctgtaaaag
    23821 cggaatgatc tttttctgga atgcgatctt caattgttta aacgcagctt cttcatcacc
    23881 ggcatcgagt gcatttttta ccggcataaa gaacgcatgc cccagcgtat gttcacgatc
    23941 gtaaagagcc tcgatgcggc tattgagttt ctctaacaac gactcgagct ctatgccttt
    24001 caccttagct tcactcagta aagagagatc aggcatcatt tcgacaaagt caaagcgacg
    24061 gcgcaaagcc gtgtccatca gagctaaaga acggtccgct gtattcatgg ctccgatgat
    24121 atcgacattg ccgggtacgc tgaagtgatc accgctataa gccagttgca ggctcatcgc
    24181 attgggcatg cctgcacgct tgtctacttc aatgagtgag attagttcac caaagatctt
    24241 ggatatgtta ccgcgattga tctcatcaat gaaaatggcg tagcgatgtc ctggatcggc
    24301 attcgcacgt tggcaaaggc gcataaagat acccggctca atgggataag agatattgcc
    24361 actctcgtca gagcgagcgc gtataccttc aataaattct tcatagccgt aagattggtg
    24421 gaacgtaacc accgcaaaac gctggatggc ttcggcagat ttagggccac gtttaagttc
    24481 ggcgtagagc tctaccaaat cctccacttg ctcgagctgt gaatcaacta gaaaccattc
    24541 gctgttatct gttttgttaa atacggcagg ctcacgccga ttcttataat caacggttaa
    24601 cgactcggga actgtatagg attgcaaagc tgcccaggca gtattcgata gattgccatt
    24661 acgaccgttt aataatgcct tacgttgaaa ccacatatgt tcaataattt ggcgaacttt
    24721 cgctcgtttc ccaagatcga gcagcaccag cgttataacc tgcatccagt tcaacgattc
    24781 aaggcgagaa tccaaccagg catcacgatc agcgggaaca gcatgagaag tgtactcttt
    24841 cattttttgc tgcaacctga aggtcttgcc agtgccaggg gggccaaaga agatcacgtt
    24901 agttggtgaa tgtaggatga tcttgttctc gatgggctca ctgcgggtta tggatgtagt
    24961 gctagctact ttctcctgct tttgttcatt atcgcgttca cggagcaatt catagagatg
    25021 ccagatggcc atattcacat aataaggatc tgtatcagga gattgggcgt gtatggcttg
    25081 tttcaattca tagtttttct gtaaccaatt tccggtcagt accaaaccga gatggaagtg
    25141 ctgatttaag aattctgctg ctttattaaa agcattctca tccacagtac tggtgtagtt
    25201 ttctggcgag aacgcggcaa agacgcgatt tctcaaactc caatacatac gcttgagaag
    25261 tccgttttct ttagcttgtt gaagtgcatt gccaacatat tggtaagttt cttcatccgg
    25321 ctgttgccga attcgttcag ttagttctct aagcagtggc aggctttgct gatattctgc
    25381 taaggatgga acgccctggc gaatacttgc aatgccgttg tcacgctcat accaaagctg
    25441 tttgaccaga ccatctgttg caggctcagc aaaagttgag gtttgctgta gcaaagtcgt
    25501 aaatgcacgg tatcgctgat accatctgga agctgtgtaa tttttatctt tcgccagggc
    25561 atcaatcaat tcactaatcg ttagcctacc tgcttcgaca ctatctacaa gagtcatcgt
    25621 gacttttcct ctgttgtgac attacttatt ttttaattaa tatgaggtat tctaactata
    25681 ttgagttaat cgttgtgaca agtgcctgtt atatatacaa ctatctgtta actcagctct
    25741 aatttaaata caatgtaagg ttgtagggtg aatgctgtta tcaacctcac cccctaaaaa
    25801 cctctttccg atgccactcc acaaacccct cataagggta atttctacgc acctgcggca
    25861 acgcaatagt tttcccgtca aaatcccaga ataatctctc aacgatccct ccgccgttaa
    25921 ccgcatccga gaccagtacc cgcatatttt catccaggcc aatcgatcct ttatcaaagg
    25981 ctttatggtg aattgcgcaa agcgccaggc cattaggaat ttcacaagga cctccttgct
    26041 gtttccattt tatatgcgcc gcttccaacg ctacggtggt gtcgtcgtgg cgcatattaa
    26101 agccacatat cgcgcactgg tagttataag cgcgtaatac atttttgcga aataaagggt
    26161 cgcgctgttt acggatttgc tggagatcaa aacccagctc gtcagcgatt tcttcctgaa
    26221 tactctcggt gaagtgcgct tcgagtatct gttgcgccag agtattgatg agttttttat
    26281 tgccggttac cagggcgtag tgctgttcat cgaaaccgcc tgcaacgtgg tattcgttca
    26341 gttccttcac tggcggttgt cggctgctcc ctgctgttga gcaaagctcg gcattgtgta
    26401 attgccagaa tccgtcgcct tgtaatcgcc agaacggcat atcaggtcgg tactgtgaac
    26461 gctgtggccc aaaacgttcc agtaaactgt gcagaggttc gtagatttcc gatccataat
    26521 cgaaaaggcg tggatgtcca ttcaggtatc ccgctaatac gtatagcaat aacaatggct
    26581 tatgcggcgc gcgctgttca cccttgtgcc agatttttat gttggcaatt gcctgctgta
    26641 gcgatttact ggaagccatt gatatccctg gcagtgaaat aattgcgatc atgctcgcaa
    26701 attttctgct gttcaagcct gcaagtgaag atatattacc ttgatctcat ttgttttttt
    26761 gctgcctgtg taccctaatt gccatgcgat gctgcaccgg cgcaaaccac cgtttacgcc
    26821 ggagcagtta aaagcgttga tggatgcgaa taaatcaaat taatgaacgc tcacttttcg
    26881 cgccaaaact gcaaaattgc ttctacaaac ctgtcagaaa taaccccccg acgggctgcc
    26941 gtgctgaaat gactgcttcg cagtgccgtc gtccagttct ccggtgaccg gtacgctgaa
    27001 ccgtaatatc ccgtgcttaa atcgtccgga tcttcatcag gcgcgttcgt cagtcctctt
    27061 tcaccttgtt gctggaagtc gacgaaaata atattggcta aaacattatt ttgataattg
    27121 ccataaatag cttcatacga atgcgggaaa ttttctttcc agtaccaggt ggtatcgccg
    27181 caaaaccacg gcgcgtcagt aatattatta agctgagaat ggtattgttt tagatcccta
    27241 cgaaaggctt caaccatatg attaaagtgt tgagggtgtg acgcgtagtc actggtcatt
    27301 aagtcaaatt cgccttgcat ccagcatacg ccgaggaatt tgttctgcgg attttttgcc
    27361 agtgcggctc gcgttctgct gactaaatcc tggtacagcg gagtatccgt tccccaacga
    27421 caagcatcat ggctggctcc gtgccgttct gaatatgtcc cttcgctgcc cgcggtaaaa
    27481 gccgatccgc cacggcaaca tggaacgatg agaacccctg cattatcagg aataaagggt
    27541 agtaatttcc gtgcaatatg cagtgcctgg ccaacggtgc cgtactgtgt ttgatgattc
    27601 gttgccagag gatggtgata accctgcata tcctgaacat cgtgtgggca gtgagtcagt
    27661 ggaataatgt cgttaaagtg acatgacggg cctccgggat gcgtatgcgc aaatctcgct
    27721 aattgtttaa ttctgggatg aggcgcatct tccctgtccg gtaatggcag tccttcgcca
    27781 tacgccatgg cattagactg accagcaacg gtaagaacat agtaataatc gggcgatatt
    27841 attgcgttca tataaatttc gcgtaattga aatgacaaat tgatagcagg gcccctttac
    27901 cggggccctg aagcgttaat tttgtactgt gactttatta tccttcacag agatcaaaac
    27961 tgaatccgtc accgctttgc cgcctgcagt ttcaccgcca ataatcaata gactattatt
    28021 ccagggcaat gatactccgt aggcccgacc ttgcgataat tcacccgatt tatcccattt
    28081 cccgttatgc caaagatgaa tatcagtgct atatgatttt ttcaggcctt catgcgcata
    28141 gttctttccg ttctggtaat tttctcgtga acctttgaat ccggcccctc cggcaaatat
    28201 aagagaatca ttgcttatcc ccgcaaaacc gccagcgacg ccatctggtg atgagacggg
    28261 agcaagctta ttccatttta aattattacc ggtgaaatca agttcaaata cggcatccgt
    28321 tcgcaatcct ggtttggctt cgccattaat aagccaggtt ttatcacctt tattcacaac
    28381 cgccgcacca gccgttccgt accagggaga ttcgccagcg taactccatt gctgtgttga
    28441 gggatcaaaa gacaacagaa acttattgaa gaaataatct tctgcttttt tgtcaaaata
    28501 atgggcattg atcttgtcta tagttgctga atcttttcca gcctcgttga gatcttcaaa
    28561 atagccattg aagatattct ggttaacacc gccagtaaca taagccttgc cgttgtgtac
    28621 aaaagtcaca tgacccgcca tgcccatcgg tgcatgcgac atcaatttaa cccaactatt
    28681 ggttttgggg ttgtatttgt gtacgtcatt aaatacctga gtcaagccct tgctattttt
    28741 gccaatgccg ccaaacacat acagattgcc atcaataaat gccgaggttg cttgatctct
    28801 tggtccacca gggaatgcag ctaacgctgt ccatttttta tctttggcct gtgtatccag
    28861 cttgtaccat gccgtacctg cgctacctaa accaatgtag acagtgtcgt tatcaattgc
    28921 tccggtacca cttttaaatg gcacaggagt ttcaggtaat acagacgcgt ttgcggcaaa
    28981 tgaagccatc atgatagcaa gcgccgttat tgttttattc attgtgactg tctcctgtct
    29041 actacagttt aaatgacaca ccaatgcgat aactgttttc cgataaatta tctctgccgt
    29101 tgtaaacacc ctgacggtcc aggtagtcat attctatgta tggcgtaata tcgggcgtca
    29161 tatggtattg tagaacaaat gcattttccg tcgcccattt cttatggttt gcatagcgat
    29221 aatcgttctg tttgctgtat agcgtcgttt gccatgcgaa ggtgaaatca ctattaatat
    29281 ggtaagtgac atatccatcc caacgatgaa cgttatcacg agacatatca ccggataagt
    29341 cttgttgtcg gtaagctttc cagtcgtaac gatagcgaat gccaaaatta agatcttttg
    29401 tcgcgtccca ggacagtttt acgtagggtc cgtagcgtgt gccgttgctg ctaaaatgcg
    29461 ttaacattcc cgggcgcacc gtccattgat catcaagttt aatcgcgtaa ttaacttcaa
    29521 cctgaacatc attgagtgcg gcattttcct ttttattatc atgaatggta ttccaggtat
    29581 tactttccat gcttgcccac catccatttt gccatccctc actgactttg agtcgagtct
    29641 cataggcgtg gcttccacta cgatatccac cacgtacgtc cagtgtcgca gcctgagaaa
    29701 ttaatgggga cgaaaagcac agtaataata cgccagaaag tattttagcc tttttcataa
    29761 atttcactca tttgtaggat acagaaagca atacaaagcc cgcataaaca attagcattt
    29821 atgttgtgta atattttttt gccaggctta tagtgtcttt ggcaaccggt agctgtattt
    29881 tatatttttt tgtataatgt ctcctgtgaa aaatctcttt tcacattatt taaataaaca
    29941 gagatccaga ttaaatacct gagtataaaa tctcttctga tgtttaattg atttgaatgt
    30001 tcgtaagcta tatcacttac tcaatccatt ttacccagag tcataaaata gaatgtctgt
    30061 tgatctcatt ttcatattcc tttcagactg aagtgcgttt tcatcacccg atgagaaaga
    30121 actgaagtga tgtttgccat agattgcgag atgccctgtc tcattgacta tcttggtgca
    30181 cctttatacc tgttatacca gatcaaaaat cacgcaatcc atacaacaaa accagatttg
    30241 caattcgtgt cacaaaatat gtcgatattt ttctaagaag aagatgccat gtgaagccag
    30301 acgaacactt gcggaggcct tcacaaacca aagattgttg tttaattatc tgttttataa
    30361 gcaatttatt taaagtataa acaaggaaag atttgcaagc taactcagtg agcgtggtga
    30421 aaatcagtgt ttacccgcca tcaggctgag cataattctc atcatgaaat atgtttcctg
    30481 gtttgtggct tgtaactggt cacttctgaa gtcgatctgg agaggcttgt tgatgttggt
    30541 gttttcagga tgatgtttca cttagtttgt ttgccgtatc gcccggcgaa tggctgtgat
    30601 tgaggaaggt ttaagtcgta gtgaccaaag ctatatttac caacgaatgt agatgaaaaa
    30661 atcatctcct gcgttcccca tatctccagg ataaaaagga atgtaacaat ctcatggcgt
    30721 aagctgacga atcagccagg aataatcgct agggacctaa gaattagcat gataatagcc
    30781 actaagaaat tactgcgctc catgaaatag ccattttgtg gcaaatggag ttgactaata
    30841 atgtcatatg tgagacggct agttgaacga atattaaatt ttgctgaatt ttttatgttg
    30901 attttacttg ttacagaaca tatcacatga tatatagata agattagttg cattaatgat
    30961 gagggttatt attagattcg tatccgattg ataaatatat aaaggtacat agcatgcaag
    31021 agcatggcgt ttgtatggca acgttattat aattaactgt tgctactcca tttaagttca
    31081 ctcagaagaa ctggtccact tacgttagtt attaagcaaa cgttcgcttt tataaacata
    31141 atcaggatta aaatgttgga ttattgctaa cccagcacag ctagtgcgcg tctgtaatta
    31201 taagggaaaa cgatgaagaa taaggctgat aacaaaaaaa ggaacttcct gacccatagt
    31261 gaaatcgaat cactccttaa agcagcaaat accgggcctc atgcagcacg taattattgt
    31321 ctgactttgc tttgttttat tcatggtttc cgggcgagtg aaatttgtcg attgaggatt
    31381 tcggatattg atcttaaggc aaagtgtata tatatccatc gattaaaaaa aggcttttca
    31441 acaacgcacc cgctattgaa caaagaagtt caggctctaa aaaactggtt gagtatccgt
    31501 acttcgtacc cgcatgctga gagcgagtgg gtatttttat cgcgtaaggg gaatccgctt
    31561 tctcggcaac agttttacca tattatctcg acttccggtg gtaatgccgg gttgtcactg
    31621 gagattcatc cgcacatgtt acgccattcg tgtggttttg ctttggcgaa tatgggaata
    31681 gatacgcgac ttatccagga ttatcttggg catcgcaata ttcgtcatac tgtctggtat
    31741 accgccagca atgcagggcg tttttacggc atctgggata gagcaagagg acgacagcgt
    31801 cacgctgttt tatagagata gttgagatac cagggatggt gttaaaaact acattattct
    31861 tacga
//
LOCUS       DAZGSP010000057        31821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZGSP010000057 DAZGSP010000000
VERSION     DAZGSP010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.057"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(97..1761)
                     /gene="glnS"
                     /locus_tag="ACHK74_002840"
     CDS             complement(97..1761)
                     /gene="glnS"
                     /locus_tag="ACHK74_002840"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFP0221626.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1964..3910)
                     /gene="nagE"
                     /locus_tag="ACHK74_002841"
     CDS             complement(1964..3910)
                     /gene="nagE"
                     /locus_tag="ACHK74_002841"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFP0221627.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4243..5043
                     /gene="nagB"
                     /locus_tag="ACHK74_002842"
     CDS             4243..5043
                     /gene="nagB"
                     /locus_tag="ACHK74_002842"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP0221628.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5104..6252
                     /gene="nagA"
                     /locus_tag="ACHK74_002843"
     CDS             5104..6252
                     /gene="nagA"
                     /locus_tag="ACHK74_002843"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP0221629.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6261..7481
                     /gene="nagC"
                     /locus_tag="ACHK74_002844"
                     /gene_synonym="nagR"
     CDS             6261..7481
                     /gene="nagC"
                     /locus_tag="ACHK74_002844"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFP0221630.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7529..8281
                     /gene="nagD"
                     /locus_tag="ACHK74_002845"
     CDS             7529..8281
                     /gene="nagD"
                     /locus_tag="ACHK74_002845"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFP0221631.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8538..10202
                     /gene="asnB"
                     /locus_tag="ACHK74_002846"
     CDS             8538..10202
                     /gene="asnB"
                     /locus_tag="ACHK74_002846"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFP0221632.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10583..10659
                     /locus_tag="ACHK74_002847"
     tRNA            10583..10659
                     /locus_tag="ACHK74_002847"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10617..10619,aa:Met,seq:cat)
     gene            10668..10752
                     /locus_tag="ACHK74_002848"
     tRNA            10668..10752
                     /locus_tag="ACHK74_002848"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10702..10704,aa:Leu,seq:tag)
     gene            10776..10850
                     /locus_tag="ACHK74_002849"
     tRNA            10776..10850
                     /locus_tag="ACHK74_002849"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10808..10810,aa:Gln,seq:ttg)
     gene            10885..10959
                     /locus_tag="ACHK74_002850"
     tRNA            10885..10959
                     /locus_tag="ACHK74_002850"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10917..10919,aa:Gln,seq:ttg)
     gene            10975..11051
                     /locus_tag="ACHK74_002851"
     tRNA            10975..11051
                     /locus_tag="ACHK74_002851"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11009..11011,aa:Met,seq:cat)
     gene            11100..11174
                     /locus_tag="ACHK74_002852"
     tRNA            11100..11174
                     /locus_tag="ACHK74_002852"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11132..11134,aa:Gln,seq:ctg)
     gene            11212..11286
                     /locus_tag="ACHK74_002853"
     tRNA            11212..11286
                     /locus_tag="ACHK74_002853"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11244..11246,aa:Gln,seq:ctg)
     gene            complement(11440..12615)
                     /gene="ubiF"
                     /locus_tag="ACHK74_002854"
     CDS             complement(11440..12615)
                     /gene="ubiF"
                     /locus_tag="ACHK74_002854"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFP0221633.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDITWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPILKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12761..14185
                     /gene="miaB"
                     /locus_tag="ACHK74_002855"
     CDS             12761..14185
                     /gene="miaB"
                     /locus_tag="ACHK74_002855"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFP0221634.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14338..15378
                     /locus_tag="ACHK74_002856"
     CDS             14338..15378
                     /locus_tag="ACHK74_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFP0221635.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICITAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15375..15842
                     /gene="ybeY"
                     /locus_tag="ACHK74_002857"
     CDS             15375..15842
                     /gene="ybeY"
                     /locus_tag="ACHK74_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFP0221636.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15932..16810
                     /gene="corC"
                     /locus_tag="ACHK74_002858"
     CDS             15932..16810
                     /gene="corC"
                     /locus_tag="ACHK74_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFP0221637.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16835..18373
                     /gene="lnt"
                     /locus_tag="ACHK74_002859"
     CDS             16835..18373
                     /gene="lnt"
                     /locus_tag="ACHK74_002859"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFP0221638.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIVSLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLLGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(18451..20328)
                     /locus_tag="ACHK74_002860"
     CDS             complement(18451..20328)
                     /locus_tag="ACHK74_002860"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFP0221639.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSGESWRLLSSVFLHSSVSHLLMNMFALLVVGGVVERILGKWRLLI
                     VWLFSGVFGGLISACYALRESEQIVISVGASGAIMGIAGAAIATQLASGAGTHHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSFILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            20783..21691
                     /gene="gltI"
                     /locus_tag="ACHK74_002861"
     CDS             20783..21691
                     /gene="gltI"
                     /locus_tag="ACHK74_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFP0221640.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            21861..22601
                     /gene="gltJ"
                     /locus_tag="ACHK74_002862"
     CDS             21861..22601
                     /gene="gltJ"
                     /locus_tag="ACHK74_002862"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFP0221641.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            22601..23275
                     /gene="gltK"
                     /locus_tag="ACHK74_002863"
     CDS             22601..23275
                     /gene="gltK"
                     /locus_tag="ACHK74_002863"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFP0221642.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            23275..24000
                     /gene="gltL"
                     /locus_tag="ACHK74_002864"
     CDS             23275..24000
                     /gene="gltL"
                     /locus_tag="ACHK74_002864"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFP0221643.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            24118..25053
                     /gene="rihA"
                     /locus_tag="ACHK74_002865"
     CDS             24118..25053
                     /gene="rihA"
                     /locus_tag="ACHK74_002865"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFP0221644.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            25137..26807
                     /gene="hscC"
                     /locus_tag="ACHK74_002866"
     CDS             25137..26807
                     /gene="hscC"
                     /locus_tag="ACHK74_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFP0221645.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDEARECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(26867..28318)
                     /gene="djlC"
                     /locus_tag="ACHK74_002867"
     CDS             complement(26867..28318)
                     /gene="djlC"
                     /locus_tag="ACHK74_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFP0221646.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVGEADNEDKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQVEEPESEDARYYQCAQRLYCGEGESLLAELCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRLLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(28315..29022)
                     /locus_tag="ACHK74_002868"
     CDS             complement(28315..29022)
                     /locus_tag="ACHK74_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP0221647.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            29124..29678
                     /locus_tag="ACHK74_002869"
     CDS             29124..29678
                     /locus_tag="ACHK74_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP0221648.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(29688..31115)
                     /locus_tag="ACHK74_002870"
     CDS             complement(29688..31115)
                     /locus_tag="ACHK74_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFP0221649.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEDIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(31112..31819)
                     /locus_tag="ACHK74_002871"
     CDS             complement(31112..31819)
                     /locus_tag="ACHK74_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP0221650.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDARDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
BASE COUNT         7930 a         8220 c         7957 g         7714 t
ORIGIN      
        1 gcagaaaaaa cgccgcagag tgcggcgttt gggattgttt gctatttctt gccggatgcg
       61 acgtaaatgc ctcacccggc ctggtagaac ttaaaattac tcgcctactt tcgcccaggt
      121 atcacgcagc ccaacggtgc ggttaaatac cggtttttcc gctgtggaat ggcggctgtc
      181 gaggcagaag taaccttcac gctcaaactg gaatgcttta cccgcaaccg catctttcag
      241 cgacggttca gcaaagccct gtttgatcac cagcgattcc gggttaatca ccgacaggaa
      301 atcatccgca gcaccagggt tcggcacgct gaacagacgg tcgtacagac ggatttcaac
      361 cggcagcgca tgtgccgcgc ttacccagtg aataacgcct ttcactttac gaccatctgc
      421 cggatccttg cttaaggtgt cggcgtcata agtacagaag atggtggtga tattgccttc
      481 ggcatctttc tccacgcgtt cagccttaat gacgtaagca ttacgcagac gcacttcttt
      541 acccagcacc agacgtttgt actgcttgtt agcttcttcg cggaaatcgg cgcgatcgat
      601 ccaaatctca ccgctaaacg gcacctgacg gctccccatt tccggtttgt tcggatggtt
      661 cggcatggtg accatttcgc cttcgccctg atagttttcg ataaccagtt tcaccggatc
      721 gataaccgcc attgcgcgcg gcgcattttc gttgagatct tcacggatgc aggattccag
      781 cgacgccatc tcaatggtgt tgtcctgctt ggtcacgcca atgcgtttgc agaactcacg
      841 aatagaagcc gcagtgtaac cacgacgacg cagaccagaa atggtcggca tacgagggtc
      901 atcccagcct tcaacgtgct tgtcggtcac cagcaggttc agcttacgct tggacatcac
      961 agtgtattcc agattcaggc gcgagaattc gtactggcgc gggtgaacag gaatggtgat
     1021 gttgtccagc acccagtcgt acaggcgacg gttgtcctgg aactcaagcg tacacagaga
     1081 gtgcgtaata ccttccaggg catcgctgat gcagtgggtg aagtcgtaca tcgggtagat
     1141 gcaccacttg ttgccagtct ggtggtgttc agcaaattta atacggtaca gcaccggatc
     1201 gcgcatcacg ataaacggtg aagccatgtc gattttcgca cgcaggcagg ctttaccttc
     1261 ttcaaaacca ccggcacgca ttttttcgaa cagcgccagg ttctcttcaa cgctgcggtc
     1321 gcggtacggg ctgtttttac ccggttgcgt cagggtgccg cggtattcgc ggatctgttc
     1381 cggcgtcagt tcatcaacgt acgccaggcc tttattgatc agttcgatcg cataggcgtg
     1441 gagctgatca aaataatcgg aggagtaacg gacgttacca gaccagtgaa aacctaacca
     1501 ctctacgtcg tttttgatcg actcaacata ctcgatatct tcttttaccg ggttagtgtc
     1561 gtcgaaacgc aggttgcact ggcctttata gtcctgggcg atcccgaagt tcaggcagat
     1621 agatttcgca tggccaatat gcagatagcc attcggctcc ggcgggaaac gggtgtgtac
     1681 tgtggtgtgc ttaccactgg ccagatcttc atcgatgatc tgacggataa agttagtcgg
     1741 gcgggcttct gcctcactca tcgtggattc ctcaaagcgt aaacaacgta taacggcgta
     1801 tgatcttata agcgggacag gctgacaact gttagttttt taaaattgat aatctaacaa
     1861 ggattatggg gatgattgat gaaaaaaaaa gcggcagaga agatctccac cgcttgtcat
     1921 tgttggatgc gacgctcaag cgtcgcatca ggcataaagc agattacttt ttgatttcat
     1981 acagcggtgt ttgacccgcc acaacatggc cctgagcttt aatgatcaag ccactgaaat
     2041 cgtcgatatt gctgcaaacc accgggctaa tcatcgagcg ggcgttagcg ttcaggtaat
     2101 ccagatccat ttccagaatc ggttgccctg cgcttacctg cgcaccctct tccaccagac
     2161 gtttaaagcc tttaccttcc agcgctacgg tgtcgatacc catatggacg acgatctccg
     2221 cgcctttttc ggtttccagg cagaacgcgt ggttggtgtt gaagattttc acgattgtcc
     2281 ctgcggctgg tgatacgacg attttatctg tcggtttcac cgccacaccg tcacccaccg
     2341 ctttgctggc gaatgcttcg tcaggaacct gatccagtgc cacgacatca ccggtaatcg
     2401 gcgataccag ctccgcgata gataccgcgt ttggtacagc ctgcggtttt gctacaggcg
     2461 cggcagttgc cggagttgct tcagctgacg cagcggctac cggaccacgg gcaacgactt
     2521 tcttcatcgc atcgccgatg gattctgctt tcgcgccaac aatcacctga atagtctgtt
     2581 tgttcagttt cactacacca gaagcaccca gacgtttaca catcgtatcg ttgacgcggg
     2641 cagagtcagc cacggtcagg cgcagacggg tgatacaggc gtcaattgct ttcaggttgt
     2701 cagtgccgcc aaccgcagca atatagttgg tcgccagttg attgaggcct tcttcagtgt
     2761 tgctgttggc ttcttcagta acgatctcgt cttctttatc ttcacgaccc ggcgttttca
     2821 ggttgaacat gcggataacc aaactgaaca ccacgaagta gatagcgaag aagacaacac
     2881 ccataaccag cagcatccag acgttctggc tggcggccgg caggttatac atcaacgcgt
     2941 agtcgatagc ccccgcagag aaagagaagc cagcatggat acccagcagt gttgccacaa
     3001 acaggctgat accggtcagc agtgcgtgca ggaggtacag cagcggtgca aggaacatga
     3061 acaggaattc cagcggctca gtcacaccgg tcaggaacgc agtaacagca acagaaagca
     3121 gcatcccgcc aaccatcgga cgacgctctt tcggtgctgc gaagtacatc gccagcgccg
     3181 cacccggcag accgaacatc ataatcggga agaagccgga catgaacatc cccgcggtgc
     3241 cgtcaccggc atagaagcgg ttgatatcac cgtggaaaac cgtacccgcc gcgttggtga
     3301 attcaccaat ctggaaccag gcgatggtgt tcagcacctg atgcagaccg gttgggatca
     3361 gcagacggtt gatgaaacca aagataccgg aacccagcgc gcccgcagaa acgatccact
     3421 cgcctcctgc atggatagcg tgctgtaccg gcggccagac gtaaccaaaa atggccgcca
     3481 gcaccaggca gaagaagccg gtggcaatcg gcacaaagcg tttgccgccg aagaaactca
     3541 ggaagtccgg cagtttaata tcggaccaac ggttataggc tgcgccacca accagaccgg
     3601 taatgatacc cgccagtaca cccatgttaa tttctgggtt gatggtcacc atcgctttgg
     3661 ttaacacaaa gtaacctacc gcacccgcca gcgccgccgc acctgcgctg tctttcgacc
     3721 agctggatgc cacaccgatg gcgaagatta atgcgaggtt atcaaaaatc gcaccgcccg
     3781 cctgggcaat aaacgcaacg ttaagtaaat ctggctgacc gaatcgcagc aacagtgccg
     3841 ccaccggcag caccgcgata gggagctgta acgccctacc gagtcgctgg aaaaaaccta
     3901 aaatattcat cttattcccc ctacgagaac cctatttggc tcgtttaaag ccgtattttt
     3961 attttgctgc aaattgtact gccgatgttc tgtaatcaga ttgttagatc atctgctaca
     4021 gagtgtgtga aaatttaatt cgtatcgcaa attaaacgcg tgtcttttgt gagttttgtc
     4081 accaaatatc gttatcatca ctccctttta ctggctaaag cagaaaactt attttatcat
     4141 tcaaaaaatc aggtcggatt gacgcctgtc tgcgcaaatc caggttacgc ttaaagatgc
     4201 ctaatccgcc aacggcttac attttactta ttgaggtgaa taatgagact gatccccctg
     4261 actaccgctg aacaggtcgg caaatgggct gctcgccata tcgtcaatcg tatcaatgcg
     4321 ttcaaaccga ctgccgatcg tccgtttgtt ctgggcctgc cgactggcgg cacgccgatg
     4381 accacctata aagcgttagt cgaaatgcat aaagcaggcc aggtcagctt taagcacgtt
     4441 gtcaccttca acatggacga atatgtcggt ctgccgaaag agcatccgga aagctactac
     4501 agctttatgc accgtaattt cttcgatcac gttgatattc cagcagaaaa catcaacctt
     4561 ctcaacggca acgccccgga tatcgacgcc gaatgccgcc agtatgaaga aaaaatccgt
     4621 tcttacggaa aaattcatct gtttatgggc ggtgtaggta acgacggtca tattgcattt
     4681 aacgaaccgg cgtcttctct ggcttctcgt actcgtatca aaaccttgac tcatgacact
     4741 cgcgtcgcga actctcgttt ctttgataac gatgtcaatc aggtgccaaa atatgccctg
     4801 actgtcggtg ttggtacatt gctggatgcc gaagaagtga tgattctggt gctgggtagc
     4861 cagaaagcac tggcactgca ggctgcagtt gaaggttgcg tgaaccacat gtggaccatc
     4921 agctgtctgc aactgcatcc aaaagcgatc atggtgtgcg atgaaccttc caccatggag
     4981 ctgaaagtta agactttaag atatttcaat gaattagaag cagaaaatat caaaggtctg
     5041 taattgttat ccctgccctg gctccttgct cagggcaata tttttttaaa atcgggggtc
     5101 agaatgtatg cattaaccca gggccggatc tttaccggcc acgaatttct tgatgaccac
     5161 gcggttgtta tcgctgatgg cctgattaaa agcgtctgtc cggtagcgga actgccgcca
     5221 gagatcgaac aacgttcact gaacggggcc attctctccc ccggttttat cgatgtgcag
     5281 ttaaacggct gcggcggcgt acagtttaac gacaccgctg aagcggtcag cgtggaaacg
     5341 ctggaaatca tgcagaaagc caatgagaaa tcaggctgta ctaactatct gccgacgctt
     5401 atcaccacca gcgatgagct gatgaaacag ggcgtgcgcg ttatgcgcga gtacctggca
     5461 aaacatccga atcaggcgtt aggtctgcat ctggaaggtc cgtggctgaa tctggtaaaa
     5521 aaaggcaccc ataatccgaa ttttgtgcgt aagcctgatg ccgcgctggt cgatttcctg
     5581 tgtgaaaacg ccgacgtcat taccaaagtg accctggcac cggaaatggt tcctgcggaa
     5641 gtcatcagca aactggcaaa tgccgggatt gtggtttctg ccggtcactc caacgcgacg
     5701 ttgaaagaag caaaagccgg tttccgcgcg gggattacct ttgccaccca tctgtacaac
     5761 gcgatgccgt atattaccgg tcgtgaaccg ggcctggcgg gcgcgatcct cgacgaagct
     5821 gacatttatt gcggtattat tgctgatggc ctgcatgttg attacgccaa cattcgtaac
     5881 gctaaacgtc tgaaaggcga caaactgtgt ctggttaccg acgccaccgc gccagcaggt
     5941 gccaacattg aacagttcat ttttgcgggt aaaacaatat actaccgtaa cggactttgt
     6001 gtggatgaga acggtacgtt aagcggttca tccttaacca tgattgaagg cgtgcgtaat
     6061 ctggtcgaac attgcggtat cgcactggat gaagtgctgc gtatggcgac gctctatccg
     6121 gcgcgtgcga ttggcgttga gaaacgtctc ggcacactcg ccgcaggtaa agtagccaac
     6181 ctgaccgcat tcacacctga ttttaaaatc accaagacca tcgttaacgg taacgaggtc
     6241 gtaactcaat aagagaaagt atgacaccag gcggacaagc tcagataggt aatgttgatc
     6301 tcgtaaaaca gcttaacagc gcggcggttt atcgcctgat tgaccagtac gggccaatct
     6361 cgcggattca gattgccgag caaagccagc ttgcccccgc cagcgtaacc aaaattacgc
     6421 gtcagcttat cgaacgcggg ctgatcaaag aagttgatca gcaggcctcc accgggggcc
     6481 gccgcgctat ctccatcgtc accgaaaccc gcaatttcca cgcaatcggc gtacggcttg
     6541 gtcgtcacga cgccaccatc accctgtttg atctcagcag caaagtgctg gcagaagaac
     6601 attacccgct gccggaacgt acccagcaaa cgctggaaca tgccctgttg aatgccattg
     6661 ctcagtttat tgatagctac cagcgcaaac tgcgcgagct gatcgcgatt tcggtgatcc
     6721 tgccagggct tgttgacccg gacagcggca aaattcatta catgccgcat attcaggtag
     6781 aaaactgggg gctggtagaa gcactggaag aacgttttaa agtgacctgt ttcgttggtc
     6841 acgatatccg tagtctggcg ctggcggagc actacttcgg tgcaagtcag gattgcgaag
     6901 actccattct ggtgcgtgtc catcgcggaa ccggggccgg gattatctct aacgggcgca
     6961 tttttattgg ccgcaacggc aacgtcggtg aaattggcca tattcaggtc gaaccgctgg
     7021 gtgaacgctg ccactgcggc aactttggct gcctggaaac tatcgctgcc aacgccgcta
     7081 ttgaacaacg ggtgttgaat ctgttaaagc agggctacca gagccgcgtg ccgctggacg
     7141 actgcaccat caaaactatc tgcaaagccg cgaacaaagg cgatagcctg gcctcggaag
     7201 tgattgagta tgtcggtcgt catctgggca aaaccatcgc cattgctatc aacttattta
     7261 atccgcaaaa aattgttatt gccggtgaaa tcaccgaagc cgataaagtg ctgctccctg
     7321 ctattgaaag ctgcattaat acccaggcgc tgaaggcgtt tcgcactaat ctgccggtgg
     7381 tacgttctga gctggatcac cgctcggcaa tcggcgcttt tgcgctggta aaacgcgcca
     7441 tgctcaacgg tattttgctc cagcatttgc tggaaaatta atgtgctttt atagtggcgc
     7501 ttattgttgt caatattctg ggtagtccat gaccattaaa aatgtaattt gcgatatcga
     7561 cggcgtgctg atgcacgata acgtcgccgt accgggtgca gcggaatttt tgcacgggat
     7621 tatggataaa ggcctgccgc tggtgttgct gaccaactat ccttcgcaga ccgggcaaga
     7681 tctggcgaac cgctttgcca ctgccggtgt cgatgtgccg gatagcgtgt tttatacctc
     7741 tgcgatggcg actgccgatt tcctgcgtcg ccaggaaggc aagaaagcgt atgtggtggg
     7801 cgaaggcgca ctgattcatg aactgtacaa agccggtttc actattaccg atgtgaaccc
     7861 tgattttgtg attgttggcg aaacgcgttc ctacaactgg gacatgatgc ataaagcggc
     7921 ctatttcgtc gctaacggtg cacgctttat cgccaccaac ccggacaccc acgggcgcgg
     7981 tttttatccc gcttgtggcg cgttgtgtgc agggattgag aaaatctccg ggcgcaaacc
     8041 gttctatgtt ggtaagccca gcccgtggat catccgcgcg gcattaaaca aaatgcaggc
     8101 gcattcggaa gaaacggtga ttgtcggcga taacttgcgc accgacattc tggcgggctt
     8161 ccaggcgggt ctggagacga ttctggtgct ttctggtgtt tcatcgctcg acgatatcga
     8221 cagtatgccg ttccgcccaa gctggattta cccgtcggtc gctgaaatcg acgttatctg
     8281 aatagcaacc acgtctcagg gcgtggtttc ttatttttac ccatcattaa aatcatccca
     8341 ttcaataaaa ctcgccaaaa attatcgatc catcaataaa tacatcacca caccctcccc
     8401 tttttcacaa atcagcaatc accattacgt tttttatttt tgcactgcca aatcgcttgc
     8461 gcacggtgct gctttgcggc atttttttaa acaagcaaac acaacaagca acaaatacca
     8521 ggttaacgga gaaggttatg tgttcaattt ttggcgtatt cgatatcaaa acagacgcag
     8581 ttgagctgcg taagaaagca ctcgagctgt cacgcctgat gcgtcatcgt ggcccggact
     8641 ggtccggtat ttatgccagc gataacgcca ttctcgccca cgaacgtctg tcaattgttg
     8701 acgttaacgc gggggcgcaa cctctctaca accaacaaaa aactcatgtg ctggcggtaa
     8761 acggtgaaat ctacaaccac caggcactgc gcgccgaata tggcgatcgt tatcagttcc
     8821 agaccggatc tgactgcgaa gtgatcctcg cgctgtatca ggaaaaaggg ccggaatttc
     8881 tcgacgactt gcagggcatg tttgcctttg ccctgtacga cagcgaaaaa gatgcatatc
     8941 tgattggtcg cgaccatctt gggatcatcc cactgtatat gggctatgac gagcacggtc
     9001 agctgtatgt ggcctcagaa atgaaagccc tggtgccagt ttgccgcacg attaaagagt
     9061 tcccggcggg gagctatttg tggagtcagg acggcgaaat ccgttcttat tatcatcgcg
     9121 actggttcga ctttgatgca gtgaaagata acgtgaccga caaaaacgag ctgcgtcagg
     9181 cactggaaga ttccgttaaa agccatctga tgtctgacgt tccttacggt gtgctgctct
     9241 cgggtggtct ggattcctca attatttccg cgatcaccaa gaaatacgcc gcccgtcgcg
     9301 tggaagatca ggaacgctct gaagcctggt ggccgcaatt acactccttt gctgtaggtc
     9361 tgccgggttc accggatctg aaagcagccc aggaagtggc aaaccatctg ggcacggtgc
     9421 atcacgaaat tcacttcact gtacaggaag gtctggatgc catccgcgac gtgatttacc
     9481 acatcgaaac ctatgatgtg acaactattc gcgcttcaac accgatgtat ttaatgtcgc
     9541 gtaagatcaa ggcaatgggc attaaaatgg tgctgtccgg tgaaggttct gatgaagtgt
     9601 ttggcggtta tctttacttc cacaaagcac cgaatgccaa agaactgcat gaagagacgg
     9661 tgcgtaaact gctggccctg catatgtatg actgcgcgcg cgccaacaaa gcgatgtcag
     9721 cctggggcgt ggaagcacgc gttccgttcc tcgacaaaaa attccttgat gtggcgatgc
     9781 gcattaaccc gcaggataaa atgtgcggta acggcaaaat ggaaaaacac atcctgcgtg
     9841 aatgttttga gtcatacctg cccgcaagcg tggcctggcg gcagaaagag cagttctccg
     9901 atggcgtcgg ttacagttgg atcgacaccc tgaaagaagt ggcggcgcag caggtttctg
     9961 atcagcaact ggaaactgcc cgcttccgct tcccgtacaa cacgccaacc tcaaaagaag
    10021 cgtatctgta ccgggagatc tttgaagaac tgttcccgct tccgagcgcc gctgagtgcg
    10081 tgcctggcgg tccttccgtc gcgtgttctt ccgctaaagc gattgagtgg gatgaggcgt
    10141 tcaagaaaat ggacgatccg tctggtcgtg cggttggcgt tcaccagtcg gcgtataagt
    10201 aagaaatgcg aaacagcccc ggtgaaatcc ggggctgttt cagttattga ggtgcttgtt
    10261 aattctgata tgcagtaagg aaatgttccg ggtttgccac atcactgttt gacaactcat
    10321 cactgtaggc cagtatttca tagattaatt tcccaccact attgttgaat tccgaatttc
    10381 atcctgtacg acaaaagaat ggctgtaggg tggggaaaaa acacaaagta aacaataatt
    10441 gacgaatata gcgccacgct gttcgcaacc taaccaaaca gtcactttcg agcaattttc
    10501 cttgaaaaag aggttgacgc tgcaaggctc tatacgcata atgcgccccg caacgccgat
    10561 aaggtatcgc gaaaaaaaag atggctacgt agctcagttg gttagagcac atcactcata
    10621 atgatggggt cacaggttcg aatcccgtcg tagccaccat cttttttgcg ggagtggcga
    10681 aattggtaga cgcaccagat ttaggttctg gcgccgcaag gtgtgcgagt tcaagtctcg
    10741 cctcccgcac cattcaccag aaagcgttgt acggatgggg tatcgccaag cggtaaggca
    10801 ccggtttttg ataccggcat tccctggttc gaatccaggt accccagcca tcttcttcga
    10861 gtaagcggtt caccgcccgg ttattggggt atcgccaagc ggtaaggcac cggtttttga
    10921 taccggcatt ccctggttcg aatccaggta ccccagccat cgaagaaaca atctggctac
    10981 gtagctcagt tggttagagc acatcactca taatgatggg gtcacaggtt cgaatcccgt
    11041 cgtagccacc aaattctgaa tgtgtcgaat acgttcggca aattcaaaaa ccaatttgtt
    11101 ggggtatcgc caagcggtaa ggcaccggat tctgattccg gcattccgag gttcgaatcc
    11161 tcgtacccca gccaatttat tcaagacgct tacatcgtaa gtgcgcccag ttggggtatc
    11221 gccaagcggt aaggcaccgg attctgattc cggcattccg aggttcgaat cctcgtaccc
    11281 cagccacatt aaaaaagctc gcttcggcga gctttttgct tttctgcgtt cattcaatgt
    11341 cgaatgcgat gttgacacgt cttatccttc aatgtcggat gcgacgctgc cgcgtcttat
    11401 ccgacctacg gttggcacgc atccggcaat gttgtaaggc tacaacccta acgcatattt
    11461 cagcgcctga cgtttcaaca cgccagcacg ctctgccgcc attaacccga gattacgcac
    11521 aaaacgcagc ggcggcagat tattactgaa tccggcataa aacagatcca taccactttg
    11581 cataatgaag ttatccgcca tgcgccgcat ctggtaacgc ttgaggatcg gataactggc
    11641 ccacgcttcg ccgtagctgc gggcgttgac cagaacatca atcagggcat cgacatcacg
    11701 ataaccaaga ttcaccccct gccccgccag cggatggatg gtatgcgcgg catcgcccac
    11761 cagcgcaagc cctggctgca catactgcaa cgcatggcga cgcgtcagcg gaaacgcacc
    11821 agcggcaagc ggtgtaacgt aacccagacg cgacgggaaa tgcttcgcga tttccgcctg
    11881 gagctgcgcc atattcatat tctgcaactg gcgaatacgc gccggagagt cataccacac
    11941 cagcgatgcc cagttatcaa acaacggcag aaacgcacgc ggtccgtccg gggtaaattg
    12001 ctgccaggtg atgtcgccgg gatcgttctc acactgtacg ctaatcaaca tacacgactg
    12061 cgcatactgc caggcatgaa cgccaattcc cgccatctgc cgcacctgcg aatttgcgcc
    12121 gtcggcacca atcaccagtt tcgcgcggat cgtttcaccg cccttcagtt ccagctcctg
    12181 aagatcatta tctcgatgta atgcaatcag cgaggttggc acacgtaacg ttactttcgg
    12241 atgcgcttcc agcgcctgcc acagcgcctg ttgcaggacg gtgttttcca ccatatagcc
    12301 aagcagcggt agcttaagtt cagcggcgtc aaacaccaca tgcgccgttt cccactccca
    12361 cgtttccagt ctgcggtaag gatggcaacg catagcctgt actgcatccc agacccctaa
    12421 ccctttaagc aatgataccg aagccgcgct gatcgccgag atccgcacgt ccggttggct
    12481 atcagcgaca aacggcgctg gttctgcgtg ctcgatcacc gttaccgaaa atccgtgctg
    12541 tgccagcccc agcgccagtg cgccgccgac cattcctccg ccgacaatgg caatttccgt
    12601 tggttgattt gtcatggtca atcatcctgt tagcaatatg ctttaagttt acaggatttt
    12661 tacccccaag ggctgataac gctcatactg gtcacaacgg cagcaaagca ttacactatg
    12721 cgccctgcat tcctggctac tatttcgcaa gagcaagtcg atgaccaaaa aactccatat
    12781 taaaacctgg ggctgtcaga tgaacgagta cgattcatcg aagatggccg atctgctgga
    12841 tgccacccac ggctatcaac tgaccgacgt ggcggaagaa gcggatgtgc tgctgctgaa
    12901 cacctgctca atccgcgaga aggctcagga aaaagtcttc catcagttgg gtcgctggaa
    12961 actgttaaaa gagaagaatc cagacctgat tatcggcgtc ggtggctgcg tggcatcgca
    13021 agaaggcgag cacattcgcc agcgcgccca ctatgtcgat attatttttg ggccgcaaac
    13081 gctgcaccgt ctgccggaga tgatcaactc cgtgcgcggc gaccgcagcc cggttgtaga
    13141 tatcagcttc ccggaaatcg agaagtttga ccgtctaccg gaaccgcgcg ctgaagggcc
    13201 gaccgcgttt gtctccatca tggaaggctg caataaatat tgcacctact gtgtggtgcc
    13261 ttacacccgt ggtgaagagg taagccgtcc gtccgacgat attctgtttg agatcgccca
    13321 gcttgcggct cagggcgtgc gtgaagtcaa cctgctcggt cagaatgtga atgcctggcg
    13381 tggtgagaac tacgacggca ccaccggatc gtttgccgat ctgttgcgtc tggttgctgc
    13441 gatcgacggg atcgatcgta ttcgctttac caccagccat ccgatcgaat tcaccgacga
    13501 tatcatcgaa gtgtatcgcg acacgccgga gctggtgagc ttcctgcatc tgccggtaca
    13561 aagcggttcc gatcgcattc tgaacctgat gggccgtact cacacggcgc tggagtacaa
    13621 agcgatcatc cgtaaactgc gtgcggcgcg tccggatatt cagatcagtt ctgacttcat
    13681 tgttggcttc cctggcgaaa ccaccgaaga cttcgagaaa acgatgaagc tgattgccga
    13741 cgtcaatttc gacatgagct acagctttat cttctctgca cgtccgggta caccagccgc
    13801 cgatatggtt gatgatgttc cggaagaaga gaagaagcaa cgtctgtata ttctgcaaga
    13861 gcgcattaat cagcaagcga tggcgtggag ccgccggatg ctcggcacca cccagcgtat
    13921 tctggtagaa ggtacatcgc gtaagagcat catggagctt tccgggcgta cggaaaataa
    13981 ccgcgtggtc aacttcgaag gtacgccgga tatgatcggt aaattcgtcg atgtagaaat
    14041 taccgacgtc tacccgaact ctctgcgcgg taaagtggtg cgtactgaag atgaaatggg
    14101 tctgcgcgtg gcagaaacac cggaatcagt gattgcccgt actcgcaaag aaaacgacct
    14161 tggcgtgggt tattatcaac cgtaattctc aggcctgccg ttccggtggg ccttgttttt
    14221 tctctcttta tccccatctt tatcgcaatg cttgcgcctt tttcctgtgg cgtgaataat
    14281 ttccttatag gccggtgaat gttcagcttc cggcggcata cagataagag gaacggtttg
    14341 aacatagaca ctcgcgaaat caccctggag ccagcagaca acgcgcgtct gttgagcctg
    14401 tgcggcccgt ttgatgacaa catcaagcag ctcgaacgcc gtctcggcat cgagatcaat
    14461 cgccgcgata accactttaa actgaccggc cgtccgattt gcatcaccgc tgcggcagac
    14521 attctgcgta gcctgtatgt cgatacagcc ccgatgcgcg gtcagattca ggatatcgaa
    14581 ccggaacaga tccaccttgc gattaaagaa gcgcgagtac tggagcaaag tgcggagagc
    14641 gtgccggagt acggcaaagc ggtcaatatc aaaaccaaac gcggcgtaat taagccgcgc
    14701 acgccaaacc aggcgcagta catcgccaat attctcgacc atgacattac cttcggcgtt
    14761 ggcccggcgg gtacgggtaa aacctacctg gcagtcgctg cagcagttga tgccctggag
    14821 cgtcaggaaa ttcgccgtat tctgctgact cgtccggcgg tcgaagccgg tgagaaactg
    14881 ggcttcctgc ctggcgattt aagccagaaa gtagacccgt atctgcgccc actgtacgac
    14941 gcgctgtttg aaatgctggg ctttgagaaa gtcgagaaac tgattgagcg caacgttatt
    15001 gaagtcgcgc cgctggccta tatgcgtggt cgtacgctga atgacgcctt tatcattctc
    15061 gatgagagcc agaacaccac catcgaacag atgaagatgt tcctgacccg tatcggtttt
    15121 aactcaaaag cggttatcac cggcgacgtc acacagatcg acctgccgcg taatactaaa
    15181 tcaggcttac gtcacgccat cgaagtgctg gccgatgtcg aagagatcag ctttaacttc
    15241 ttccacagcg aagacgtggt tcgtcaccct gtggtggcgc gtatcgttaa cgcctatgaa
    15301 gcctgggaag aagccgaaca aaaacgaaaa gcggcgctgg cggcagaacg caagcgcgaa
    15361 gaacaggaac aaaaatgagt caggtgatcc tcgatttaca actggcatgt gaagataatt
    15421 ccgggttacc ggaagagagc cagtttcaaa catggctgaa tgcggtgatc ccgcagtttc
    15481 aggaagaatc ggaagtgact attcgcgtgg tcgataccgc cgaaagccac agtctgaatc
    15541 tgacctatcg cggtaaggat aagccgacca acgtgctctc cttcccgttt gaagtgccgc
    15601 ctggcatgga gatgtcgcta ctgggcgatc tggttatctg ccgtcaggtg gttgagaagg
    15661 aagctcagga gcaaggcaaa ccactggagg cgcactgggc gcatatggtg gtgcacggca
    15721 gtctgcattt gttaggttac gatcacatcg aagatgacga agcagaagaa atggaagccc
    15781 tcgaaacaga gattatgctt gctctgggct atgaggatcc gtacattgcc gagaaagaat
    15841 aatttgccag gcatttgact ggcagacgtc cccgccgttg gtgattaacg acggcgtttt
    15901 gattaactaa catgagaacc catacgacgc catgagcgac gacaattcac acagtagtga
    15961 cacgataagc aacaagaagg gatttttctc cctgttactc agccaacttt tccacggtga
    16021 accgaaaaac cgtgacgaac tgctggcgct gatccgtgat tccgggcaga acgaccttat
    16081 cgacgaagat acgcgcgata tgctcgaagg ggtgatggac atcgcagacc aacgcgttcg
    16141 cgacatcatg atcccccgct cccagatgat taccctgaaa cgcaaccaga cgctggacga
    16201 atgccttgat gtcatcatcg agtccgccca ctcacgtttc ccggtgatta gcgaagacaa
    16261 agatcacatt gaagggattc tgatggcgaa agacttgctg ccgtttatgc gcagcgatgc
    16321 tgaagccttc agcatggaca aagtgttacg tcaggcggtt gtcgttcctg aaagtaagcg
    16381 cgtagaccgg atgctgaaag agtttcgctc tcagcgttac cacatggcga tcgttattga
    16441 cgaattcggt ggggtttccg gtctggtgac cattgaagac atcctggaac tgattgttgg
    16501 tgagattgaa gacgagtatg acgaagaaga tgatatcgac ttccgtcagc tgagtcgtca
    16561 tacctggacc gtgcgcgcac tggcttccat tgaagacttc aacgaagcgt tcggcaccca
    16621 ctttagcgat gaagaagtcg acactatcgg tggtctggtg atgcaggcgt ttgggcatct
    16681 tccggcgcgt ggcgaaacta tcgacatcga cggttaccag ttcaaagtgg cgatggccga
    16741 tagtcggcgt attattcagg ttcatgtcaa aatcccggat gactcacccc agccaaagct
    16801 ggatgaataa aaccgaaact ggatagataa ctacatggct tttgcctcat taattgaacg
    16861 ccagcgcatt cgcctgctgc tggcgttatt attcggtgcc tgcggaacgc tggccttctc
    16921 tccttacgat gtctggcctg cggcgattgt ttcgctgatg gggcttcagg cgctgacctt
    16981 taaccgccgc ccgctccagt ctgccgctat tggcttctgc tggggatttg gcctctttgg
    17041 cagcggtatt aactgggtct atgtcagcat cgcgaccttt ggcgggatgc ctggcccggt
    17101 taacatcttc ctggtggtgc tgctggcggc gtatttgtcg ctgtataccg gactgtttgc
    17161 tggcgtgctg tcgcgcctgt ggccgaaaac cacctggctg cgcgtggcga ttgccgcccc
    17221 tgctctctgg caagtgaccg agtttctgcg cggttgggtg ctgactggct tcccgtggtt
    17281 acagttcggc tatagccaga ttgatggccc gttaaaaggg ctggcaccaa taatgggcgt
    17341 ggaagccatt aacttcctgc tgatgatggt tagcggcctg ctggcactgg cgttagtcaa
    17401 acgcaactgg cgtccgctgg tggtggccgt cgtgctgttt gcccttccct tcccgctgcg
    17461 ttacatccaa tggtttaccc cgcaaccgga gaaaaccatt caggtttcga tggttcaggg
    17521 cgatattccg caatcactga aatgggacgg agaccagctt cttaatacgc tgaagattta
    17581 ctacaacgca acggcaccgc tgctgggcaa atcatcgttg attatctggc cggagtcggc
    17641 gataaccgat ctggaaatta atcagcaacc gttcctcaaa gcactggacg gtgagttgcg
    17701 tgataaaggt agctcgctgg taaccgggat tgtcgacgcg cgtctcaata agcagaaccg
    17761 ctacgatacc tacaacacca tcatcacgct gggtaaaggt gcaccgtaca gctacgaatc
    17821 agccgatcgc tataacaaaa accatctggt gccgtttggc gagtttgtcc cgctggagtc
    17881 gattctgcgt ccgttagcgc cgttctttga tctgccgatg tcgtcgttca gccgtgggcc
    17941 atatatccag ccgccgctgt cagtaaatgg tattgagctt actgcggcta tttgctacga
    18001 gatcattctc ggcgagcaag tgcgcgataa cttccgcccg gataccgact atctgctgac
    18061 tatctccaac gatgcgtggt ttggtaagtc tattggtcca tggcagcact tccagatggc
    18121 gcgaatgcgt gcgctggagc tggcgcgccc actgttgcgc agcaccaaca acggcattac
    18181 ggcggtgatt ggcccgcagg gtgagattca ggcgatgatc ccgcagttca cccgcgaggt
    18241 gttgaccact aatgtgacac cgaccaccgg actcacaccg tatgcacgta ccggcaactg
    18301 gccgctgtgg gtgctgacgg cgctgtttgg ttttgccgct gtgctgatga gtctgcgagc
    18361 acgtaaacgt tgatgtgcct gatgtatcct gcatgagcaa aacgtacttt ctcattgagc
    18421 tttggggcat ggttatcatg cccctttttc ttacggttca gggatacaag ccacattgcc
    18481 atattgggga tgacgaataa ttgcctgcgg atcaagcgag cgcgtcctgt ccatttggat
    18541 caacccgttc ttcgccatga caaatatttt tctaccatcc gctgaaaaac gaaggtgatt
    18601 aacgtagctg gcaggataaa cgccaataat ctgcccggca ttaatattga gcaggatgac
    18661 ataaccttgc aggccatatg acaccaacgc taaatattga ccgtccgggc taataccctc
    18721 aacggaataa acgctcctgc tacaccattg cgtcataatg ctggcttgtt gatcccctac
    18781 gcttatacgt gaaatcccct gactatcggc aaaaataacc tcgccgtttt caggtcgaaa
    18841 acgcatcgcc gatatcgttc cacgcgcatc acctgcggga aagtcttctt tctgatgcgt
    18901 gatggtattg atccgcacga tgttacctct gcctgctccc ggaaccagta atgttctttc
    18961 atccggggaa agtgccataa cccaggcgcc tggtttaccg ctcgtccccg tcccgtcgta
    19021 tttctccagc gggatatccg ccacatgctg ataagtcacc agatcaatcg ctgacacact
    19081 attactggtg cgattcatca cccaggcttt tgtgccatca cgcgagagga taaaactgtc
    19141 aggatgtgca ccggtggtaa tcgactgaat aatctcccct gtcgccacgt taatgaccga
    19201 taacgcattc gcttcaaatg acgtcgcata aatcaacttt tcatccgggc ttaatgccag
    19261 cgatctgacg ccctgatctt tacagccacc acaatgtttg actgactttt ctttcgctat
    19321 aggcgctgta aaggtatgca ggattttctt ttgtccgagg tcgaaaacaa caatgctgtt
    19381 ggtgttttca attgcggcat acaacgtatt accatcccga ctgatcgcca tatcatggat
    19441 atcggcaatc tcagcgagta aatcaccact ggcctgttca cgacttaccg gagcggataa
    19501 tgtttccctg agggcgttgc gttcctccgc taactgttgt tttttttgtc gtcgctcttg
    19561 ttcaatctcc tgcggataaa acgcttcacg caggctttgc ctgacctgta aaactgactc
    19621 atccatctgc tgctgcgcaa gccagatagc cccggtcaga agaagactgc ccgcaacaat
    19681 aatcccgcct tccgtaacga gtcggttttg cccagataaa cgcgcgctca gccaacccaa
    19741 cgcgccaccc gcaatcaggc cgccaatgtg gcaagcgtta tctattcctg tttgccgggc
    19801 accgtacaac agtgtcagcg ccaccatacc caacagagga aatactcgcc gctggttttt
    19861 atggtgtgtg cccgcacctg aagcaagctg tgttgctatc gcagcgccag ctattcccat
    19921 aattgccccg gatgccccaa cgctgatgac tatctgctca ctttcgcgta acgcataaca
    19981 ggcgctgatt agcccaccaa agacgccaga gaatagccag acaatcagga gtcgccattt
    20041 ccccagaata cgttccacca ctccccccac caccaggagt gcaaacatat tcatcagcaa
    20101 atgagaaaca ctgctgtgca gaaagacact gctcaataag cgccaggatt cacccgacaa
    20161 cgtgagagaa gcaacgttag caccgagatg gataagtgcg gattgatcga cttcttcata
    20221 actcatccat tgtgcgaggt aacaaaatat cccaacacag agcgcaaaaa ggataagggt
    20281 tattgcgggt aattgaacat ttaacggctt aaccgaagat gcagacataa ggtgattctt
    20341 ccttgaaaag acaggtaaaa agcattttcc ccgtgtgcag ttatcatccc ggcaaacgtc
    20401 ccataccgaa aagttaactc tgtccgaaat atgacttttt cagcctgaaa ttacaatcgt
    20461 aattaattca ctcctaagta ttttttggca tgtaagttgc tttatttact gtgtaagcaa
    20521 tgaaatcagc cgcgatgcaa ccaggttgca ccaccacaaa tcagcggtag caccaaaaag
    20581 gtgcggttta agaattttgc ttcggaatgg tgcggcgcat ctcgcaaaaa caaacaataa
    20641 aacagcaata tctttacatt tagccaaact aaatgttacc taacaggcac aatactgcac
    20701 aataaagttg cagatgataa caacacaaac actcacaacg ggtatccatg cgttcttaac
    20761 gcagaagata aaggagttgg atatgcaatt acgtaaacct gccacagcaa tcctcgccct
    20821 ggcgctttcc gcaggactgg cacaggcaga tgacgccgcc ccggcagcag gcagtacgct
    20881 ggacaaaatc gccaaaaacg gtgtgattgt cgtcggtcac cgtgaatctt cagtaccttt
    20941 ctcttattac gacaatcagc aaaaagtggt gggttattcg caggattact ccaacgccat
    21001 tgttgaagca gtgaaaaaga aactcaacaa accggacttg caggtaaaac tgattccgat
    21061 tacctcacaa aaccgtattc cactgctgca aaacggcact ttcgattttg aatgcggttc
    21121 taccaccaat aacgtcgaac gccaaaaaca ggcggctttc tctgacacta ttttcgtggt
    21181 cggtacgcgc ctgctgacca aaaagggcgg cgatatcaaa gattttgccg acctgaaagg
    21241 caaagccgta gtcgtcactt ccggcactac ctctgaagtt ttgctcaaca aactgaatga
    21301 agagcaaaaa atgaatatgc gcatcatcag cgccaaagat cacggtgact ctttccgcac
    21361 cctggaaagc ggtcgtgccg ttgcctttat gatggatgac gctctgctgg ccggtgaacg
    21421 tgcgaaagcg aagaaaccag acaactggga aatcgtcggc aagccgcagt ctcaggaggc
    21481 ctacggttgt atgttgcgta aagatgatcc gcagttcaaa aagctgatgg atgacaccat
    21541 cgctcaggtg cagacctccg gtgaagcgga aaaatggttt gataaatggt tcaaaaatcc
    21601 aattccgccg aaaaacctga acatgaattt cgaactgtca gacgaaatga aagcactgtt
    21661 caaagaaccg aatgacaagg cactgaacta attacaagaa ccaggggcgg aaattccagc
    21721 cctctcgatt gttacgtagc acggacagac tatacgcctg atggtcgttc cccatcgggc
    21781 ctgaaaaccg caatacgctg ggtaacaatc ttcgagggta gcagttaacg ctgctaccct
    21841 cttttttctg gagtagattt atgtctatag actggaactg gggtattttt ttacaacaag
    21901 ccccgttcgg caacaccacc tatcttggtt ggatctggag tggttttcag gtgacgatcg
    21961 ctttgtcgat ctgcgcctgg attatcgctt tcctcgtcgg ttcatttttc ggcattttac
    22021 gtaccgttcc aaaccgtttt ctctccggtc tgggtacgtt gtatgtcgaa ctgttccgca
    22081 acgtgccgtt gattgtgcaa ttctttacct ggtatctggt gatcccggag ctgttgccgg
    22141 agaaaatcgg catgtggttt aaggccgagc tggatcccaa tattcagttt ttcctttcat
    22201 ccatgctctg cctggggctg tttaccgccg cccgtgtttg cgaacaggta cgcgccgcta
    22261 ttcagtcgct cccgcgtggg cagaagaatg ccgcgctggc gatgggcctg acgctaccgc
    22321 aggcttaccg ctatgtactg ctgcctaatg cttatcgcgt tatcgtcccg ccgatgacct
    22381 ccgagatgat gaacctggtg aaaaactccg ccatcgcctc gactatcggt ctggtggata
    22441 tggcagcgca ggcgggtaaa ttgctcgact actcggctca cgcctgggag tcgtttaccg
    22501 ccattacgct ggcgtatgta ttgattaacg cggttatcat gctggtgatg acgctggttg
    22561 aacgtaaagt tcgcctgccc ggcaacatgg ggggcaaata atgtacgagt ttgactggag
    22621 ttccattgtt ccttccctgc catatctgct cgacgggctg gtgatcacgc tgaaaatcac
    22681 cgtcacggca gtcgtgattg gtattttgtg gggcaccatg ctggcggtga tgcgcttgtc
    22741 cagctttgcg cccgtagcgt ggtttgccaa agcctacgtt aacgtattcc gctcgattcc
    22801 tttagttatg gttttgctgt ggttttacct gatcgtgccg ggttttctgc aaaacgtgct
    22861 gggattatcg ccaaaaaatg atatccgttt aatttcggcg atggtggcgt tttcgatgtt
    22921 tgaagcggcc tactattcag agattatccg tgctggtatt caaagtattt ctcgtggtca
    22981 gtcgagcgcc gcgctggcgt tgggaatgac tcactggcag tcgatgaaac tgattattct
    23041 gccgcaggcg ttccgcgcga tggtgccgct gctgctcact cagggcatcg tactgttcca
    23101 ggatacctca ctggtgtatg tgttatctct ggccgatttc ttccgtaccg cctcaaccat
    23161 tggtgagcgt gatggtacgc aggtcgagat gatcctgttt gccggatttg tttatttcgt
    23221 tattagcctt agcgcgtcgt tgttggtcag ctacttgaaa agaaggacag cataatgatt
    23281 accctgaaaa atgtttcaaa atggtatggt cactttcagg tgctgaccga ctgctcaacc
    23341 gaagtgaaaa aaggcgaagt ggtggtggtt tgcggcccgt ctggttccgg aaaatcaacg
    23401 ctgattaaaa ccgtcaacgg cctcgaaccg gtgcagcaag gtgaaatcac cgtcgatggt
    23461 atcgtggtta acgacaagaa aaccgatctg gcaaagctgc gttcccgagt cgggatggtg
    23521 ttccagcatt ttgagttgtt ccctcatctg tcgattatcg aaaacctgac cctggcgcag
    23581 gtgaaagtgc ttaaacgcga taaagcgccg gctcgtgaaa aagcattgaa attgctggag
    23641 cgcgtgggac tttccgcaca tgccaataaa tttccggcgc agctttccgg cggtcagcag
    23701 cagcgtgtgg cgatcgctcg cgcattgtgt atggatccta ttgcgatgct gttcgacgaa
    23761 ccgacgtcgg cgctggatcc ggagatgatc aacgaagtgc tggacgtgat ggtggaactg
    23821 gcgaacgaag gaatgaccat gatggtggtg acccacgaaa tgggctttgc ccgcaaagtg
    23881 gcgaatcggg tgatctttat ggatgagggt aaaattgtcg aagactcgcc gaaagacgct
    23941 ttcttcgatg atccgaaatc ggaccgcgca aaagacttcc tcgcgaaaat cctgcattaa
    24001 tcactctggc gcgcataccc aagtgcgcgc catctcacgc tttatcgttt cgcccctcgt
    24061 atctgttaca tcctggcgtt acccttgtcg caaagaagca cacaacaagg agcaacaatg
    24121 gcactgccaa ttctgttaga ttgcgaccca ggtcatgacg acgctatcgc aatagttctc
    24181 gccctcgcct caccagagct tgatgtcaaa gcaattacgt cttccgccgg aaaccagaca
    24241 ccagaaaaaa ccttacgcaa tgttcttcgt atgctgacct tgctcaatcg caccgatatt
    24301 ccggtagcag gcggcgcggt aaaaccgtta atgcgtgagt tgattatcgc ggacaatgtg
    24361 cacggcgaaa gcggtctcga cggcccggca ttaccggaac cggcgttcgc accgcaaaac
    24421 tgtacggcgg tagagctaat ggcgaaaacg ctgcgtgaaa gtgcggaacc tgtcacgatt
    24481 gtgtctaccg gaccgcaaac taacgttgcc ttgctgctca atagccatcc ggaactgcat
    24541 agcaaaattg cccgtatcgt gattatgggc ggcgcaatgg ggcttggtaa ctggacgcct
    24601 gcggctgaat ttaatattta cgttgacccg gaagcggcag aaattgtctt ccagtcaggg
    24661 atcccggtgg tgatggccgg tctggatgtt actcacaaag cacaaatcca cgttgaagac
    24721 actgagcgtt tccgcgcgat tggcaaccct gtttcaacca ttgttgccga actgctggat
    24781 ttcttcctcg aatatcataa agacgaaaaa tggggctttg tcggcgcacc gctgcatgac
    24841 ccatgcacca tcgcctggct gttgaaaccg gagttgttta ccactgttga gcgctgggtt
    24901 ggcgtggaaa cacaggggaa atatactcag ggtatgacgg ttgttgatta ttattatctg
    24961 actggcaata aaccgaatgc caccgtaatg gtcgatgttg atcgtcaggg ctttgttgat
    25021 ttactggctg atcgtctgaa attttacgct taacacacgt cattgattaa gatcaccacg
    25081 cgttacctgg gtggtcttaa tctgtttatc tctttttctt tcaagatata aatgcaatgg
    25141 ataatgcaga actcgccatt ggtatcgatc tcggtactac caatagttta attgccgtct
    25201 ggaaagacgg tgccgcgcaa ttaattccaa ataagttcgg tgaatattta acaccatcca
    25261 taattagcat ggatgaaaat aatcatattt tagtcggaaa accggctgta tcacggcgta
    25321 cttcgcatcc ggataaaacg gcagcgttat ttaaacgtgc aatgggcagt aataccaact
    25381 ggcggttagg cagcgacaca tttaacgcgc cagaactgtc ctctttggta ttacgctcat
    25441 taaaagaaga tgccgaagaa tttctgcaac gtccgattaa agatgtggtg atctccgttc
    25501 cggcttattt cagcgatgaa caacgcaagc atacccgttt agcagcggag ttagccgggt
    25561 taaatgcggt acgcttaatt aatgaaccca cagcagctgc gatggcgtat ggcctgcata
    25621 cccaacaaaa tacccgttcg ctggtgtttg atctcggtgg cggcacgttt gacgttacgg
    25681 tgcttgagta cgccacgccg gtgattgaag ttcacgcctc cgctggcgac aacttcctcg
    25741 gtggcgaaga ttttacccat atgttggtcg atgacgtttt aaagcgcgcg gatgtcacca
    25801 ggaccacgct taacgagagt gaactggcag ccttgtatgc ctgtgtggaa gcggcaaaat
    25861 gtagcaatca atcgccatta cacattcgct ggcagtacca ggatgaagca cgggaatgcg
    25921 aattttatga gaatgaactg gaagatttgt ggttgccgct gctcaatcgc ttgcgagtgc
    25981 cgattgaaca ggcgttgcgc gatgcacgtc tgaagccgag tcaaatcgac agtctggtgc
    26041 tggttggcgg cgcgtcacaa atgccgctgg tgcagcgaat cgccgtgcgt ctgtttggca
    26101 aattaccgta tcaaagttac gatccgagca ccattgtcgc gctgggcgca gcaatccagg
    26161 ccgcctgccg cttacgcagt gaagatattg aagaggtaat cctcactgat atttgccctt
    26221 actcgttggg cgttgaagtt aaccgccagg gcgtttccgg cattttctcg ccgattattg
    26281 aacgaaacac cactgtgccc gtgtcgcgtg tagaaactta ttcaaccatg cacccggaac
    26341 aggattcaat tacggttaac gtctatcagg gagaaaacca caaagttaaa aacaacattc
    26401 tggtggaatc cttcgatgtg ccgttgaaga aaaccggggc ttatcagtcg attgatattc
    26461 gctttagtta tgatatcaac gggttgcttg aagttgacgt gcttctggaa gacggcagcg
    26521 ttaagtccag agtgattaac cacagcccgg taacattgag cgcgcagcag attgaagaga
    26581 gtcggacgcg gttatccgca ttgaaaattt atccgcgcga tatgctcatc aatcgcacct
    26641 ttaaagccaa actggaagag ttgtgggcgc gggcgctggg tgacgagcga gaagagattg
    26701 gccgggtgat caccgatttt gatgcggcgc tgcagtcaaa cgatatggcc cgcgtcgatg
    26761 aggtcaggcg gcgggcgagc gtttatttag ccattgagac ctcataatca cggctgccgg
    26821 atgcggcgta aatgctgcat ccggcatcgg cgcaaaaaac acatcgtcaa ctaaacaaca
    26881 tcgcgatttt gccgagagca taacctataa ccagcaccgt taaactgatt ttgataatgc
    26941 tggtaatgct ctgtggtttt tcaccctcat catttttttc ttcatggttt tcaggtgttg
    27001 gctcatcgac atcttcatcc tccggctcga gttttacttt cgtcaacagc aaccgggtga
    27061 taatggcctc taatggatga gcatctcctc cttccagcct tgtattaaga cgggccagca
    27121 tctcttgttt cggaaacgct tcaagtacta cctgttccgc tacccgatac aaatccagta
    27181 acggtgaatc ttcttctgtc tcgctaccga ctctcgattt ccaccacgcc catgctttgc
    27241 gtttgaagaa taacgactca ataaacgatt gacctaacgg cgataatttt ccgctatgta
    27301 aaatttccgc ccataacagc cgcgtccggg cgctctcacc aggaacatat ttcagcggtt
    27361 gaccttgcgc atctaccatg ctccgcgctt cgataaccat cactaataac gcgaaataat
    27421 ccgggcaatg acgcttgctc cactgcaaca acaaattatc agcttgtgta gatgggtaac
    27481 tctcccagta tgcgcagagc tcagcaagca ggctttcccc ctcgccgcag tacaaacgtt
    27541 gtgcacactg gtaataccgg gcatcttcgc tttctggttc ttccacttgc cactgtttgg
    27601 ctacaggaat gagttccgcc atacctgttt gcaccgagct gtaccagcgc aacagtttgc
    27661 gatgtaactt tgcatcatca ggaatgaacc acggaccttc catcgccaaa aacgcagtga
    27721 catagtcagg atggtaacgg cagcaacgtt ccagcgcgaa gtaatattca acagtctggt
    27781 tctgtaacgc gacgggatat tccagaaggc taaggaaatc gaaacaatca ccagcctgaa
    27841 tggcctcaag aaaagcctcc ctttcttcct catccattcc ttcgctgtca ttaaacgaat
    27901 gccagttcaa acgctctgcc agcaaagaag cacaatttaa tgaaagatat cgcgcttcta
    27961 tagcgattgc acacagcggc cagcgtaatt gatcgacatc ctccatgttc caggtattta
    28021 attgctgaat aaatttctgc caggcggagg gctgaaaacg atcactttct gaatccagta
    28081 atgtcctgaa tgcacgtagt atttcatgtt cagcggcggc atctttatct tcattatctg
    28141 cttccccgac aggatttacc gccagtcgca gggcctcttc ataggcctgg cgtaatgctt
    28201 taaacccctg cggatcggtt tcgggatgac ataacggtaa gcgagcaaga taagcctggc
    28261 ggataatgtc tatttgcgtc gtgctttcaa tttcgaggat ttgccaacat gttttcatag
    28321 gtcgctcaca tccttaagcg ttaccgggca ttcaggctgg acagttaatg gctgccaggg
    28381 aagcgtggca tagaaacgat cgcttcccgc tgccagctct tcgaacatcc gactcccggc
    28441 gctatcgtat ttgtacaggg ttagcgcctc gcgtagcgaa gtgttgtctt cgcactgaga
    28501 agattgccag tagagacgtc ctaacgaata ggcggaaaaa tagtgcatcc agctgtgata
    28561 gtaatggtga gctcgtacat agacgcgcga ttgcagccat aaactctctt cttccgtcag
    28621 ccagttgttg agcacaccga cccgtgacag aaaccccatt cgaacataat cccaggcttt
    28681 gattccccct tctccacagc acatagcggt ttgggcaacg aattgggtat aaatcaaccc
    28741 tctctccgag ccaccagcaa ttaatgcttt ccactcttca ggggaagagc gatgccatgt
    28801 cagatataag ccagctaaat cattggcatg accatcatca gtcatctgat aaatagtctg
    28861 aattaaatca tcacgattat ttattcccca ggatgatgag agatcgaatt cttttatacc
    28921 ggtatacatt tttggcgagt catgcgcggg tttgcaaccc tgattaagta tttccatggg
    28981 cgcagaaagc gcaaaaagaa gatattgttc ttccttattc atttgttaat aatcccttaa
    29041 gtgcattaac tgactacttt tttattattg atgagtataa acttgacgct ttcaaaataa
    29101 aaagaaaatc gaagcattca cacatgaata aaaaattaat gtatatattc gcaattttta
    29161 tagttgcagc aattacctgt attagccaac ccaagaaaac gacgttgcgt gataaagcca
    29221 tggtgaatta tgcctttgat tatttaagat caccgggcag tcttccattc accacggcag
    29281 ccacggagct ttccgcgatt catggtcact caacgtcgca atatcgcctt ggagaatttt
    29341 atcttcatgg tagcgacggt aaaccactgg attatacaca ggcgagatac tggtatgagc
    29401 aatcagcgga acaggaaaat ccacgcgcgc aaagtaaact ggggtggatc tacctcaaag
    29461 gtctgggggt caaacccgac acccgtaaag caattctctg gtataaggaa gcagctgaac
    29521 aagggtatgc tcatgctcaa tatactttag gtttgatcta cagaaatggc tcaggtatta
    29581 atgttaacca ttatgaatct caaaaatggt taaaactggc cgccaaacaa cattacaaaa
    29641 atgcggaaag attacttgcc gggcttcccg cacattaacg tcgactgtca cccgaacaga
    29701 tggagtattt tccctatcaa accagcaaag ataaaaatat agaaaataat tttaatgatg
    29761 cccagacagc ctggcccacc atcgtcaacg ggcattggct gcggttgcgg ctccggttca
    29821 acagtttgct tctcggtaaa attcgccgcc tgaattaata atcgacgaat aattaatgct
    29881 tccagtggac aacgatcttc cgcatcaagg cggctcacaa tcggcattaa tgattcttct
    29941 gtcggttggc tagcctgcac taattgtttg gcaactaaat ataatggcca taagggatgt
    30001 tctgtatcac gcgtgagtga gatccgcggc ttacgtcctt tgtgcaaaac catctcgacc
    30061 agtgaacgcc ccagcgcaga gagtttttca tcgctgagta tttcgtataa atgaaaacgc
    30121 gtacgggcgc tgtcgccggg gacataaagt agctgtttac cctgatcgtt aaccagttca
    30181 cgagcttcaa tcatcatcac cactaacggg taataatcga cccgatgctg acggcaccat
    30241 tgcaatatta aagcatccgc ctgggtggag ggatactcgc gccagtagtc acaaagttct
    30301 gccagcaagc tttcgccttc tccacaatag acgcgttgcg cgtattcata ataacgcgca
    30361 tcttcattat caggttcagc ttcccgccac gaccgggcgt aatccagaag ttcggggata
    30421 tccaggcgac ctgcgctata ccagcggagt aaatttctgt gcagttttgc atcatcgggg
    30481 atgatcactg cgcgatgttg cgctaaatac aatgtcagcc attctggatg cgatgaccaa
    30541 atccgagcga acatttggta aaaatcgatg gtgtcatttt gcacggcaac gggcagatgc
    30601 agaatggtct ggaagttaaa aacattcccc ttcgcaatgg catataaaaa ggattccagt
    30661 tcttcttcgt ctatttcccc gacgttttcc tcctgcaacc accgcaatct ttctgctaat
    30721 aacaccacgc actcgaagga taaatgggca gtgttcatgg ctattgtgca cagcgaccag
    30781 cgtaattcat caatatcttc catcgagcaa taatttaatt gctgaatgaa tcgctgccag
    30841 gcagagggca gaaaacgttc gctatcagag gcaagtaacg cacgaaaggc gagcagaatt
    30901 tcatgttctg ctacctcata ttcttctggt tgccaaacag atttagcagg cgactgcgca
    30961 atccgtagcg cctcctcata cgcctgacga agttgtttaa aaccctgcgg atcggtttct
    31021 ggatgaaagg acggtaacag cgccagataa gcgcggcgga taatatcgac gtcagtcgtt
    31081 tcctctattt cgagaatctt ccagcaattt ttcataggtc gctcatatcc ttaagcgttt
    31141 ccgggcatgc agaatagtaa gccaatggtt gccaggacaa ggtagggtaa tacgcatcat
    31201 cactggcaaa taattgataa aacatatcat tgccggaatc gtcgtattct ttacgcgcta
    31261 agaattcgcg taataactgc agatgatcat cttcgggaga ctgccaatat tgccgaccaa
    31321 aggtataacc ggcaaagtat tgccgccagt tacgataata acgtagagcc cgtagatgaa
    31381 tgcgcgactg gatccataga ctttcttctt cgctcaacca gttattaagt acgcccattc
    31441 ggcttaaaaa acccatccgg acataatccc aggctttaat tcctccctca ccacagcatt
    31501 cggcggtgct ggcgacaaat tgcgcataag cctgaccttg ctcatttagt tcagcaagat
    31561 agtcacgcca ttcacatggc gaataacgaa accagcggtg ataaaaccct gccaaccgag
    31621 cggcatgacc gttatcgatc attcggtgaa ttgtgcgcag caggtcatca cgggcatcaa
    31681 ttccccagga ggaagaaagg tcaaaaaagc atgtacctaa atacattttg ggtgatgaat
    31741 gcgaaggtaa acagcattca ttgcgtattt ccatcggagt ggaaagtgca aacaatattt
    31801 tttgcgactc catgtccata t
//
LOCUS       DAZGSP010000058        31512 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZGSP010000058 DAZGSP010000000
VERSION     DAZGSP010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.058"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(52..348)
                     /gene="yaaX"
                     /locus_tag="ACHK74_002872"
     CDS             complement(52..348)
                     /gene="yaaX"
                     /locus_tag="ACHK74_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFP0221651.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(562..1848)
                     /gene="thrC"
                     /locus_tag="ACHK74_002873"
     CDS             complement(562..1848)
                     /gene="thrC"
                     /locus_tag="ACHK74_002873"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFP0221652.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1849..2781)
                     /gene="thrB"
                     /locus_tag="ACHK74_002874"
     CDS             complement(1849..2781)
                     /gene="thrB"
                     /locus_tag="ACHK74_002874"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFP0221653.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2783..5245)
                     /gene="thrA"
                     /locus_tag="ACHK74_002875"
     CDS             complement(2783..5245)
                     /gene="thrA"
                     /locus_tag="ACHK74_002875"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFP0221654.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(5271..5390)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(5326..5391)
                     /gene="thrL"
                     /locus_tag="ACHK74_002876"
     CDS             complement(5326..5391)
                     /gene="thrL"
                     /locus_tag="ACHK74_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFP0221655.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(5605..6291)
                     /gene="yjtD"
                     /locus_tag="ACHK74_002877"
     CDS             complement(5605..6291)
                     /gene="yjtD"
                     /locus_tag="ACHK74_002877"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP0221656.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(6691..6831)
                     /gene="yjjY"
                     /locus_tag="ACHK74_002878"
     CDS             complement(6691..6831)
                     /gene="yjjY"
                     /locus_tag="ACHK74_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFP0221657.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            6927..7643
                     /gene="arcA"
                     /locus_tag="ACHK74_002879"
     CDS             6927..7643
                     /gene="arcA"
                     /locus_tag="ACHK74_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFP0221658.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(7703..9055)
                     /gene="creD"
                     /locus_tag="ACHK74_002880"
     CDS             complement(7703..9055)
                     /gene="creD"
                     /locus_tag="ACHK74_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFP0221659.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGELSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQITDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(9113..10537)
                     /gene="creC"
                     /locus_tag="ACHK74_002881"
     CDS             complement(9113..10537)
                     /gene="creC"
                     /locus_tag="ACHK74_002881"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFP0221660.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNSADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(10537..11226)
                     /gene="creB"
                     /locus_tag="ACHK74_002882"
     CDS             complement(10537..11226)
                     /gene="creB"
                     /locus_tag="ACHK74_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFP0221661.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(11239..11712)
                     /gene="creA"
                     /locus_tag="ACHK74_002883"
     CDS             complement(11239..11712)
                     /gene="creA"
                     /locus_tag="ACHK74_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFP0221662.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            11923..12792
                     /gene="robA"
                     /locus_tag="ACHK74_002884"
     CDS             11923..12792
                     /gene="robA"
                     /locus_tag="ACHK74_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFP0221663.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(12789..13436)
                     /gene="gpmB"
                     /locus_tag="ACHK74_002885"
     CDS             complement(12789..13436)
                     /gene="gpmB"
                     /locus_tag="ACHK74_002885"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFP0221664.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            13488..14009
                     /gene="yjjX"
                     /locus_tag="ACHK74_002886"
     CDS             13488..14009
                     /gene="yjjX"
                     /locus_tag="ACHK74_002886"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFP0221665.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(14094..14420)
                     /gene="trpR"
                     /locus_tag="ACHK74_002887"
     CDS             complement(14094..14420)
                     /gene="trpR"
                     /locus_tag="ACHK74_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFP0221666.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(14510..16447)
                     /gene="sltY"
                     /locus_tag="ACHK74_002888"
     CDS             complement(14510..16447)
                     /gene="sltY"
                     /locus_tag="ACHK74_002888"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFP0221667.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMILSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRHGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            16658..18325
                     /gene="ettA"
                     /locus_tag="ACHK74_002889"
     CDS             16658..18325
                     /gene="ettA"
                     /locus_tag="ACHK74_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFP0221668.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(18632..19864)
                     /gene="nadR"
                     /locus_tag="ACHK74_002890"
     CDS             complement(18632..19864)
                     /gene="nadR"
                     /locus_tag="ACHK74_002890"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFP0221669.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(19885..21267)
                     /gene="radA"
                     /locus_tag="ACHK74_002891"
     CDS             complement(19885..21267)
                     /gene="radA"
                     /locus_tag="ACHK74_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFP0221670.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(21316..22284)
                     /gene="serB"
                     /locus_tag="ACHK74_002892"
     CDS             complement(21316..22284)
                     /gene="serB"
                     /locus_tag="ACHK74_002892"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFP0221671.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            22390..23034
                     /gene="ytjB"
                     /locus_tag="ACHK74_002893"
     CDS             22390..23034
                     /gene="ytjB"
                     /locus_tag="ACHK74_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFP0221672.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            23062..24078
                     /gene="lplA"
                     /locus_tag="ACHK74_002894"
     CDS             23062..24078
                     /gene="lplA"
                     /locus_tag="ACHK74_002894"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFP0221673.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQKCEALLVDFPEQEKELRELSTWIA
                     GAVR"
     gene            24110..24373
                     /locus_tag="ACHK74_002895"
                     /pseudo
     CDS             24110..24373
                     /locus_tag="ACHK74_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(24534..25253)
                     /gene="deoD"
                     /locus_tag="ACHK74_002896"
     CDS             complement(24534..25253)
                     /gene="deoD"
                     /locus_tag="ACHK74_002896"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP0221674.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(25333..26556)
                     /gene="deoB"
                     /locus_tag="ACHK74_002897"
     CDS             complement(25333..26556)
                     /gene="deoB"
                     /locus_tag="ACHK74_002897"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP0221675.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(26608..27930)
                     /gene="deoA"
                     /locus_tag="ACHK74_002898"
     CDS             complement(26608..27930)
                     /gene="deoA"
                     /locus_tag="ACHK74_002898"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFP0221676.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(28057..28836)
                     /gene="deoC"
                     /locus_tag="ACHK74_002899"
     CDS             complement(28057..28836)
                     /gene="deoC"
                     /locus_tag="ACHK74_002899"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFP0221677.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            29094..30644
                     /gene="yjjI"
                     /locus_tag="ACHK74_002900"
     CDS             29094..30644
                     /gene="yjjI"
                     /locus_tag="ACHK74_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFP0221678.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            30616..31479
                     /gene="yjjW"
                     /locus_tag="ACHK74_002901"
     CDS             30616..31479
                     /gene="yjjW"
                     /locus_tag="ACHK74_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFP0221679.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT         7479 a         8685 c         7942 g         7406 t
ORIGIN      
        1 ccagcgtcgc atcaggcgct gaatgccgga ttgttacccg gcatttgtca tttagcggtg
       61 atgtttgcct ggaccatgac cgccgtgatg atcatgagga gctttcttat ggtggcgcgg
      121 cggtggcggc ggtccgtgtg ggtgccagcg attgcctcgc cattcataat gttgtttcca
      181 ccagccgtgg tcgcgccagt gacctccatc ccagtaatag ccacgattat cacgatcgcc
      241 tatctgtaat tttactgacg ggactaacgt aatttccgca gcctgtgctg ccatgggagc
      301 gaccagaacc agggaaagtg cgagtacgat agattgcatc tttttcacag gttactcctt
      361 ttatcattgc ccgttatggg tcgatatccg gagattacgc gatgagaacg acggttgtta
      421 ttctccgcaa tcctaatctc taagttaccg catttattga gaatttctcc tttttccctg
      481 tcgtttttct ccataatttc ttcataaaaa agccgggctg cataaaagca aacccggcct
      541 gattgagata atgaatagat tttactgatg attcatcatc aatttacgca acgcagcaaa
      601 atcggcgggc agattatgtg aaagcaaggg taaatcagca cgttctgcca gctcttttgg
      661 cagatccaac gtttcaccga gaatcgcttc cacgctctct ttaaatttcg ccggatgcgc
      721 ggtgccgagg aacaagccat attcgcctgg attcaactgg tcacgcagcg cacgatacgc
      781 tacggcagcg tgcggctccg aggtgtagcc cagttctttt aactcacgca ttgtctgttg
      841 cgtggtttca tcatccacgg ctgcataacc cagctctttc agttgccaga ttttgcggcg
      901 gaacaactct tccacacgcg gccagttgtt cggctgactc acgtccatcg cgttggataa
      961 cgtcgcctga gtcgctttgg gtgaccactg accgtcgtgc aggaaacgtg gcacggtatc
     1021 gttcacgttg gtcgcagcaa taaaacgttt caccggcaga ccgagtgact tcgccagcag
     1081 acccgccgtc aaatcgccga agtttccgct tggcaccgag acaaccagct ggttgcgcgc
     1141 ttcctgcggc agctgcgcaa cagcttcaaa gtagtagcaa atctgcgcca gcaaacggct
     1201 gatgttaatc gagttagctg agtttaaccc cagcgccact ttcagttctt catcatcaaa
     1261 cgcctgcttc accagcgcct gacaggcatc gaaatcgccg tcgatggcaa cagtttcgat
     1321 attgccgccc aacgtacaga acagtttttc ttgcagtgga ctgattttgc ctcgtggata
     1381 gaggataacc actttcacat tcggtaaacc gtagaaagca tgagccactg ccgctccggt
     1441 atcaccggag gtcgcggtca gaatggtcac tggcttatcg cccgcaatat gggtcagcat
     1501 ttgtgccata aagcgaccgc cgaaatcttt aaatgccagc gttggcccgt ggaacaattc
     1561 cagacaaccg acatcgcttt caacattggc gaccggagcc gggaaggcaa acgccgcgcg
     1621 cacgcgctct tccaggattt cctgcgggat ttcatcacca ataaacgccg agaggatctt
     1681 cgcactgcgg gtgacaaaat ccagcttcag catctcatca atttcagtca ggctgaattc
     1741 cggcaggtca tgcggaaaaa acagcccctg atttttgccc aacccctggg ttacggcttg
     1801 cgcaaagctg acctgctcgt tgtgatcttt cagattgtag agtttcattt agttttccag
     1861 tactcgtgcg cccgccgtat ccagccggca aatatgaaca aaaccttcct gattttgcag
     1921 gtagttctta cccaaccagt cggcaacgcg ctgggcggta tccggcttgt cacatagagc
     1981 gaacaaggtc gggccggagc cggagatacc gctcgctacc gcgccgattt ccgcgaccgc
     2041 ctgccgcgcc tgccggaagc caggcagtaa ccgttcacgg tagggttcag cgataacatc
     2101 tttcatcagc ttcgcggcaa gctcaggctg acgggaatag caggcgtgaa tgaagccagc
     2161 cagatgtcgc ccgtgcgcaa tgcaatcctg gcggcgatac tgcgccggta aaatagccct
     2221 ggcttctgcc gtcgagactt taatccccgg atacgccagc acccacagcc actcatcaaa
     2281 ccctggcact tgctggctga tgatgtcgtt ttcttcgatc atcaactgca taccaccaag
     2341 aaaacacggt gccacgttgt cgtaatgaat gctgccggag attcgtcctt ccagctcgcc
     2401 catcaaagcc agcaaacgag tgtcattaag cggcttgcca cagtgttcat tcatcgccat
     2461 cagcgccgcg accaccgaac aggcgctgga gcctaagccc gaaccgattg gcatattctt
     2521 ttccagagtc atcgccactg gaatttgctt gccaagctcc tggcaaaaac gctcccagca
     2581 ctgataaacg atattttccc gtggttctga cggcagctta tcggcaaagc gtccgaggtt
     2641 gttgagactg aatgtctctg ccgcctcaac cgtgactaca tctccgagca atgcaccatc
     2701 aacaggtgtc accgccgccc cgagcacatc aaacccgacg ctcatattgg cactggaagc
     2761 cggggcataa actttaacca tgtcagactc ctaacttcca tgagagggta cgtagcagat
     2821 cggcaaagac accggcagct gtaacgtcat tgcccgcacc atatccgcgc agcaccaacg
     2881 gcagcggctg ataatagtgg ctataaaagg ccagggcgtt ttcgccattt ttcactttga
     2941 acagcggatc attaccatcc acttcggcaa tcttcacgcg gcaggcacca tcttcatcaa
     3001 tattgccaac atagcgcaaa acttttcctt catcacgggc cttcgccacg cgcgcggcaa
     3061 agagatcgtc gagctgtgac agattcgcca taaaagcggc aacatcaccc tcagcgttaa
     3121 actctgcggg cagcacaggt tcaatttcaa tatccgccag ctccagttca cgtcccgttt
     3181 cacgagcgag aatcaatagc ttacgcgcta catccatacc agaaagatca tctcgcggat
     3241 ccggttcggt ataacccatt tcccgcgcca gagtagtcgc ctcggagaaa ctcatgcctt
     3301 cgtctaactt gccgaagata taagaaagcg aacctgaaag aatgccggag aacttcatca
     3361 attcatcacc agcattgagc agattttgca ggttctcaat aaccggtaat ccagccccaa
     3421 cgttggtgtc atagaggaat ttacgccgcg atttttccgc cgcatgacgc aacagatggt
     3481 agtaatccat cgacgaggtg ttggcctttt tgttcggcgt gacaacgtgg aaaccttcgc
     3541 gcaagaagtc ggcatattga tccgccactg cctggctgga ggtgcagtca acaatgaccg
     3601 ggttcagcag atgatattct ttcacgaggc gaattaagcg cccgagatta aacggctctt
     3661 tggcttgcgc cagttcttcc tgccagtttt ccagatttag gccatgcaca ttggtgagca
     3721 gtgccttcga gttggcaaca ccgcagacac gtaagtcgat atgtttattc ttcagccagc
     3781 tttgttgacg cttcagttgc tccagcagcg caccgccaac gccaccgacg ccaatcacaa
     3841 acacttcgat aacctgatcg gtattgaaca gcatctgatg agtaacgcgc acgccagtgg
     3901 tcgcatcatc gttatttacc acgacagaga ttgagcgttc agaagatccc tgagcaatgg
     3961 cgacaatgtt gatattggcg cgggccagcg cggcaaagaa tttcgccgag atcccacgca
     4021 aggtgcgcat accatcacct accaccgaga taatggccag ccgttccgtc accgccagcg
     4081 gctccagtaa gccttctttc agttccaggt agaactcttc ctgcattgcc cgttcagccc
     4141 gcacacagtc gctttgcgga acgcagaaac tgatactgta ttcggaagat gattgcgtaa
     4201 tcagcaccac ggaaatacgg gcgcgtgaca tcgcggcaaa gacgcgcgcc gccatgccga
     4261 ccattccttt catccccggg ccggaaacgc tgaacattgc catgttattc agattggaaa
     4321 tgcccttgac cggtaattcg tcttcatcac gactggcacc aatgagcgta cctggtgctt
     4381 gaggatttcc ggtattttta atcaggcaag ggatctggaa ctgggcgatg ggggtaatgg
     4441 tgcgggggtg aagaacttta gcgccgaagt aggaaagctc catcgcttcc tggtaggaca
     4501 tcgacttcaa caacctcgca tcgggcacct gacgcgggtc gcaggtatag accccgtcaa
     4561 cgtccgtcca aatctcgcaa caatcggcgc gtaaacaggc agccagcacc gccgcggagt
     4621 agtcggaacc gttgcgtcca agtaccacca gttcgccttt ttcattaccg gcggtgaaac
     4681 ctgccatcaa caccatgtga tcagccggaa tgcggcttgc cgcaatacgg cgggtggact
     4741 cagcaatatc gacggtagat tcgaggtaat gccccactgc cagcagtttt tcgaccggat
     4801 cgataacggt aacgttgtga ccacgcgctt ctaacacgcc ggccataatg gcgatcgaca
     4861 ttttctcgcc acggcaaatc agcgcagcgt tgatgctatc cgggcactgc cccaacaaac
     4921 taatgccatg caggacatgt tttatttggg caaattcctg atcgacgaaa gttttcaatt
     4981 gcgccagcgg gaaccccggc tgggcggcgg cgagtcccgt caaaagttcg gcaaaaatac
     5041 gttcggcatc gctgatattg ggtaaagcat cctggccgct aatggttttt tcaatcatcg
     5101 ccaccaggtg gttggtgatt ttggcggggg cagagaggac ggtggccacc tgcccctgcc
     5161 tggcattgct ttccagaata tcggcaacac gcagaaaacg ttctgcattt gccactgatg
     5221 taccgccgaa cttcaacact cgcatggttg ttacctcgtt acctttggtc gaaaaaaaag
     5281 cccgcactgt caggtgcggg cttttttctg tgtttcctgt acgcgtcagc ccgcaccgtt
     5341 acctgtggta atggtgatgg tggtggtaat ggtggtgcta atgcgtttca tggatgttgt
     5401 gtactctgta atttttatct gtctgtgcgc tatgcctata ttggttaaag tatttagtga
     5461 cctaagtcaa taaaatttta atttactcac ggcaggtaac cagttcagaa gctgctatca
     5521 gacactcttt ttttaatcca cacagagaca tattgcccgt tgcagtcaga atgaaaagct
     5581 gaaaaatact tactaaggcg ttttttattt ggtgatattt ttttcaatat catgcagcaa
     5641 acggtgcaac attgccgtgt ctcgttgctc taaaagcccc aggcgttgtt gtaaccagtc
     5701 gaccagtttt atgtcatctg ccactgccag agtcgtcagc aatgccatga ctcgttcgcg
     5761 taaagcttgc agttgatgtt ggtctgccgt tgtgtcactt ttcgccggtt gttgtattaa
     5821 tgttgctaat tgatagcaat agaccatcac cgcctgcccc agattgagcg aaggataatc
     5881 cgccaccatc ggcacaccag taagaacgtc agccaacgct aactcttcgt tagtcagccc
     5941 ggaatcttcg cgaccaaaca ccagcgcggc atggctcatc catgaagatt tttcctctaa
     6001 cagcggcacc agttctactg gcgtggcgta gtaatgatat ttcgcccgac tgcgcgcagt
     6061 ggtggcgaca gtgaaatcga catcgtgtaa cgattcagcc agcgtcggga aaactttaat
     6121 attatcaata atatcaccag atccatgtgc gacccagcgg gtggctggct ccaggtgcgc
     6181 ttgactatcg acaatccgca gttcgctaaa acccatcgtt ttcattgccc gcgccgctgc
     6241 cccaatattt tctgctctgg cgggtgcgac cagaataatc gttatacgca tattgccact
     6301 cttcttgacc aaataaccgc gaacagggtg atcactgtca acttattacg cggtgcgaat
     6361 ttacaaatta ttaacgtaag tcgcagaaaa agccctttac ttagcttaaa aaaagctaaa
     6421 ctatttcctg actgtactaa cggttgaatt gttaaaaaat gctacatatc cttctgttta
     6481 cttaggataa ttttataaaa aataaatctc gacaattgga ttcaccacgt ttattagttg
     6541 tatgatgcaa ctagttggat tattaaaata atgtgacgaa agctagcatt tagatacgat
     6601 gatttcatca aactgttaac gtgctacaat tgaacttgat atatgtcaac gaagcgtagt
     6661 tttattgggt gtccggcccc tcttagcctg ttatgttgct gttaaaatgg ttaggatgac
     6721 agccgttttt gacactgtcg ggtcctgagg gaaagtaccc acgaccaagc taatgatgtt
     6781 gttgacgttg atggaaagtg catcaagaac gcaattacgt actttagtca tgttacgccg
     6841 atcatgttaa tttgcagcat gcatcaggca ggtcagggac ttttgtactt cctgtttcga
     6901 tttagttggc aatttaggta gcaaacatgc agaccccgca cattcttatc gttgaagacg
     6961 agttggtaac acgcaacacg ttgaaaagta ttttcgaagc ggaaggctat gatgttttcg
     7021 aagcgacaga tggcgcggaa atgcatcaga tcctctctga atatgacatc aacctggtga
     7081 tcatggatat caatctgccg ggtaagaacg gtcttctgtt agcgcgtgaa ctgcgcgagc
     7141 aggcgaatgt tgcgttgatg ttcctgactg gccgtgacaa cgaagtcgat aaaattctcg
     7201 gcctcgaaat cggtgcagat gactacatca ccaaaccgtt caacccgcgt gaactgacga
     7261 ttcgtgcacg caacctgctg tcccgtacca tgaatctggg tactgtcagc gaagaacgtc
     7321 gtagcgttga aagctacaag ttcaatggtt gggaactgga catcaacagc cgttcgttga
     7381 tcggccctga tggcgagcag tacaagctgc cgcgcagcga gttccgcgcc atgcttcact
     7441 tctgtgaaaa cccaggcaaa attcagtctc gtgctgaact gctgaagaaa atgaccggcc
     7501 gtgagctgaa accacacgac cgtactgtag acgtgacgat ccgccgtatt cgtaaacatt
     7561 tcgaatctac gccggatacg ccggaaatca tcgccaccat ccacggtgaa ggttatcgct
     7621 tctgtggtga tctggaagat taatcggctt taccaccgtc aaaaaaaacg gcgcttttta
     7681 gcgccgtttt tatttttcaa ccttatttcc agatacgtaa ttgatcgtcc attgtaactt
     7741 ctttactggc tttcattttc ggcagtgaaa acgcatacca gtcgatatta cgagtcacaa
     7801 acatcatgcc ggccagcgcc accaccagca cactggttcc caacaacagc gcgctatcgg
     7861 cagagttgag cagtccccac atcacaccat ccagcaacaa cagcgcgagg gtaaacaaca
     7921 tgctgttgcg ccagcctttc aataccgcct gcaaataaat accgttcatt aacgccccaa
     7981 tcagactggc gattatccat gccacggtaa aaccgatatg ttcagaaagc gccagcaaga
     8041 gcaaataaaa catcaccaat gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt
     8101 gcgcggtgag cgtttcaaaa acaaagaacg ccataaaagt cagtgcaatc agcagaatgg
     8161 cgtacttagt cgcccggtcc gttatttggt attgatcggc tggcgtcgtc actgcgacgc
     8221 taaacgcggg gaagttttcc cagccggtat cattgcctga agcaaaacgc tcaccgagat
     8281 tattagcaaa ccagctgctt tgccattgcg cctgaaaacc tgactcgcta acttcccgtt
     8341 tggctggcaa aaaatcacct aaaaaactgg gatgcggcca gttgctggtt aaggtcattt
     8401 cgctattacg cccggcaggc accacagaaa gttcgccggt accgcttaaa ttcagggcca
     8461 tattcagctt caggttctgc ttccgccagt ccccttcggg taaagggata tgcacgccct
     8521 gcccgccttg ctctaacccg gtgccgggtt caatggtcag cgccgttccg ttaacttcag
     8581 gcgctttcac cacaccaata ccacgcgcat ccccgacgct aatcacaata aatggcttgc
     8641 caaaggtgat atttggcgcg tcgagttcgc taagacgcga aacatcgaaa tcggctttta
     8701 gcgttaaatc actgtgccag acctgaccgg tataaatccc tatcttgcgt tcttccacgt
     8761 tctgattgcc atcaaccatc aatgactcag gtaaccaaaa atggataaaa cttcgtttcc
     8821 gctccacggt tttatcctct tcctgtaccg tataaagctc ggtcacagga atagcgataa
     8881 gcggcccaac gagtttttgc ggcccgctgg tactttggcg aatcgcatct tccacatcgc
     8941 tacggtaatc ggcacgttcg acaatcacct gccgaatcag cattatcgga atcaacaaca
     9001 gcaatactgc accaaacagg gtggtcattt tccagaacag gggggatttc aacatagtct
     9061 tctcctttgc aatggcagca ggatacgaag gctatgtggg aagaatttga agctatgtga
     9121 agtgacggtg aagtcgaagc gaggccagca cgccaccttc ctgcacgttg cgcagcgtga
     9181 cttcgccgtt aaacaaacgg gcgacctcac tgacgaacgc caaccccaga ccgctgcttt
     9241 tttgcccatt tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt
     9301 caggaatacc actaccggta tccagcacct taagcgtgac gtgttcctga tccacttcgg
     9361 cgcttagcgt tatacgaccg ctctcggggg taaaatcgat ggcgttatcc agtaaattcc
     9421 ccagcgcctg atccagtaac gccggttcag cagcaacatt aacctcggtg ggcgtaacat
     9481 gtagagtgat gtttttttct gccaactgca cggtgcgcgc ttcgctgacg cggcggaata
     9541 atgccgccac atcaacagca gtcaaaacga cttcctgacg attctccagt cttgcctggc
     9601 gtagtaacgt ttccaccagt gcctgcattc gcgcattttg cgtcagaatg ttgtcagtaa
     9661 aacgagccac tacttcaggc ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg
     9721 ccgccagtgg gctttttagc tcatgggtta acgcataaac atactgctca atatagtttt
     9781 tcccttccag cttcacgcgc atactttcca gcgcctgcgc gagtttacgc agctcgctac
     9841 taccgagatc gggaagagga acgggcttat tgtcagtgac ggaatcagca tagcgagtga
     9901 gccgggcaat ggagcggttg atccaccaaa ccatgcctgc gccaatcacc agtgcgatcc
     9961 ccaacaaaat ggcgctggcc cataaaattc gccgctcgct gcgtttaatg accggagcca
    10021 tcgccgcgtt cggtttgcct acgctcaaaa cgccaataag ccgcgagccg tccataatcg
    10081 gcgcggcaac atacatcacc gaactttcgg gatcggcaga attttgcaac gtgctgcgcg
    10141 caccatactg accacgcaac gttagccaga cgtcattcca gcgcgaataa tcctgcccaa
    10201 cggctttatt tgccgaatcg aacaatactt taccctgcga atcggtcata tagacatgat
    10261 attcattgcg cactttgtta atgccaccga tattggcgcg aaacgggcga tgttgtagct
    10321 gattaaacgc ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg
    10381 ccagctccgc cagcaacgtt gcggtgtcga tcaacgtccc ctccgttgct cttcgcacgc
    10441 ccggtttaac ttctttgaca aaaatagcca gtacgaacca ggccgccacc gccaccagta
    10501 aaaaatagcc cagcagcaac cgcatgccga tacgcattac aggcccctca ggctatatcc
    10561 catgccgcga tgagtattaa tcggtgaaag atcggggttg atggcgcgca gcttggcacg
    10621 caacgtttta atgtgggtat caacggtgcg atcgtaggtg tcctgcgcat cttcccacac
    10681 gctatccatc agttgctggc gggaccagac gcggcctggc gacttgagta acgtcttcag
    10741 caataaaaac tcataccgcg tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc
    10801 gggttcattc aattcaaaat gaccaatacg gatgacggga gacggcgacg agaacttctt
    10861 cacccgacgc agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa acggtttagc
    10921 cacatagtcg tcagcgccaa tttccagccc aagcaggcga tcgacctctt cacttcgggc
    10981 cgtcaggaac agtacaggta acgccggatg gagcgccagt aactggcggc acaattcaaa
    11041 gccactgata tcaggcaaac caacgtcaag aatcatgacg tcgggaacct gctggcgagc
    11101 tttatccagc accggcaaac ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa
    11161 catgtagacc agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga ccgtttcccg
    11221 ttgcataaat cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt
    11281 tttcggcgaa ccttctacaa ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt
    11341 ggcgtcataa aagcgcacga cctgtaacga cttaaagacc agggacgtgc gttttttgaa
    11401 tactacctcg ccctgagctt tgccgttttt aatacgatcc gacagttcaa tcggcccgac
    11461 ttgctgacaa gaaatggccg catcggaggt atcttctgcc aggcccaatc cccctttaat
    11521 accaccggtt ttcgcccggc tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa
    11581 ggcttccaca acaattttgt gatccgggcc gatcatttta aatacggtgt cgaccgaacc
    11641 aatctcttct gcatgagcca atggccccag cattatcagg cttaaagaca ggatcaaatg
    11701 cttgtatttc atattgttac catttttatc tattacacga gcaattagtt cgtcacggta
    11761 aataacatat tgattaagaa caaaatctca atacttttat ttccgtcaag ccctaaaaca
    11821 tactctacta agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta
    11881 cctgatgtca ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt
    11941 attcgcgacc ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat
    12001 gtagcggcga aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact
    12061 ggccatgcta ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta
    12121 cgcctgactg cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag
    12181 acatttaccc gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct
    12241 cctgaatgga gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca
    12301 gagcacaaat ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc
    12361 tgttcgctgg agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat
    12421 tttctcggca acgcgccgac cattccgcca gtgctctacg gcctgaatga aacgcgtccg
    12481 agtcaggata aagacgacga acaagaggta ttctatacca ctgcgttagc ccaggatcag
    12541 gcagatggct atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg
    12601 tttacctatg aaggtctggg aaccggcgtg caggagttta tcctgacagt atacggaacg
    12661 tgcatgccaa tgctcaatct gacgcgccgt aaaggtcagg atattgagcg atactacccg
    12721 gcagaagatg ccaaagcggg agatcgccca attaatctgc gctgtgaact gctgattccg
    12781 atccgtcgtt aacgctgcaa ctcatctaat gcaggggcgt ccagatgcga gatgtcccct
    12841 gcggtttcaa caacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa
    12901 atagaacagt tacgcagacg taagcggcgc tctgcccatg ctggtaatcc gagaatcgta
    12961 ctcaccagac atcccagtgc aataccatgg ctcaccagca gcggtcggct tccctgcggt
    13021 aagtcccggc aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactcg
    13081 ccttcaggaa tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct
    13141 tcttcggtca gagaatcgat atgtcttttt tccagcacac ccatgtttaa ttcacgcagg
    13201 cgagaatcaa agatgatgtc acagccgcag gcctgggcga tgatttccgc cgtacgccgg
    13261 gtgcgtccta aatcgctact gataatatgc gtaatgccaa gctctttggc acgggttgcc
    13321 acctgcatcg cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata
    13381 cgtcgctcgg cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt
    13441 tttttccgta tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg
    13501 tctgtgcgac caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct
    13561 tcggcgaagg atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacagc
    13621 cctttggcag tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt
    13681 tacttccaga ggctgatttt tgggtggcga ttgaagctgg catcgatggt gacagcactt
    13741 tcagttgggt ggtgattgaa aacaccagcc agcgcggcga ggcgcgttcg gctactttgc
    13801 cgttacctgc ggtgattctg cagaaagtgc gtgaaggtga ggcgcttggt cccgtgatgt
    13861 cgcgttatac cggtattgat gagattggtc gtaaggaagg ggcgatcggc gtgtttaccg
    13921 ccgggaaact cactcgcgcc agcgtctatc accaggcggt gatcctggcg ctgagtccgt
    13981 ttcacaatgc cgtgtatcag gcattgtagg acggataagg cgttcacgcc gcatccggca
    14041 ttcggtgcac gatgcctgat gcgacgctgc cgcgtcttat caggcctaca aaatcaatcg
    14101 cttttcagca acacctcttc cagccactgg cgcagctcaa cgggcgcggc tttcaggctg
    14161 ttagatccac gcgtaatcgt cgcgatgccc gcgccgagtt cattttttaa ctcacgctgg
    14221 ctcatttcgc cgcgcaacag ctcttcgaca atacgcacgc gagtccccaa cgcttcgcgc
    14281 tcatctggcg tcagcatcag gtttaacaac ggtaaatgga gatcgttttg gtaggcattc
    14341 ttaagcaggt cgacaaaacg taaccactcc tggtgacgct gttctgccat cgctgctgaa
    14401 tagggtgatt gttgggccat aatatgtcgc cattgttagc gggggaagca aaatgcctcc
    14461 cccggttgta ctcgctaaag agtacgatag catatcataa acgtgcggat cagtaacgac
    14521 gtccccattc cgtggcgctc atcaacgtcg gtttatcccc catgaaatag cggtagtaag
    14581 cgtcataagc cagcacgttc ttcacataac cgcgcgtctc ggagaacgga atactctcga
    14641 caaatgccac agcgtcgata cgcccggcgc tgttgccaag ccaggttcgc acccgtccag
    14701 gtccggcgtt ataagctgct gaggagaaaa tacggttatt gccaaactgc tgataaacat
    14761 attgcaggta actggtgcca atgttgatat tcgtttccgg atccagcaat tgcccagggc
    14821 tgctataacc aggaatagag aacatcttca ccgtatgggt cgctgtacca ggcataatct
    14881 gcatcaggcc gctggcccct accggtgatt tcactttcgg attccaggcg ctctcctgac
    14941 gggcaatcgc catcgcatag ctttgcggga tctctttccc actggtgtaa cgtttgaaaa
    15001 gatcgttgta agccagcggg aatcgctctt ccagatgatc ccaaagcttc ccggcgatcg
    15061 ttgcctgaac gctaagatcc caccattggt tattgaaagc ataccgagcc agttgagcct
    15121 gctctgtttt tgacttgctc ttcaccagat tggcccactc gctacgcgcg gtgttatcga
    15181 gattccagta catcaactcg cgcacgcgcg ccatctccgg cccctgagtc agggcgctgt
    15241 caacattctg cggcgcttta tcaatcttca gctcatactc ttctccgatg cgttgtgctg
    15301 caaccatcgg gtagaaacca cgctgttgca tgagttgatg caaaatctct ttcgcttcag
    15361 cttcacgtcc gcgttccagc aataaatccg cctgccagta acgccattca tctttctctt
    15421 ttgcttccat cggcagacgc gccagccagg tattcaggcc gtgacgatcg ccggtgccaa
    15481 gtgccattcg cacacggcgt tcaatcaacg acgtggattg cgagcgcata atggcgtcat
    15541 cgcgccattt cgcctgctcg tcggtgacat cgttgcccat caaacgccag gcgacgatat
    15601 cgcgcagctc ctgaatctga tcttcattaa gctgctgcgc ctgggcaagc gacaggatca
    15661 tcagccgtgc attttcagca tcctgccgcg ccacactggc aaacgccacc gctgccattt
    15721 gacgggtaaa atcggtcgct ccggttgtac gcgcgaaggt cagtaccgta ttagggttgt
    15781 tcgccagtga aatgattgcc gaggcgatag tctggtaatc ggcaggcatc tgccctgcca
    15841 gcactgttac caggccagtg ttacccgctt tcatcgccag acggatacgc tctaaatacg
    15901 ccagcggatc ttgtttacct gacgcacgcc agacgctaaa taacttgtca caggcgttag
    15961 gctggctctt gccggttagc cacagctctt tcgccccttg ccaggcttct tcactctgcc
    16021 cggtgttcca tttcgcatag tagtaattac attgcgcttc ggtagttccg ggcttttccg
    16081 ggctaaaggc taacaagcca cgccagtctt cacgccgcgc cagttcattg acgaaacgag
    16141 attgcagcgt gcgagcggga ggaagcgtgg ggttagcgcg aacaaagtta gtgaccgtca
    16201 ccgccggttg attcatcaga tcatcggtga tctggcggta ttccaggtag ggataaagcg
    16261 gataatcctt cagtccaggc atcatttgtt cgaccacatc catttgtcga ttatcccagg
    16321 cctgcttgat ttgcgcgtaa cggctacgct gctcatccag tgagtcggct cgcgccacgc
    16381 tgctgaccgt cagcagacag acaccggcag ccaacagccg ccaggtaact tgtttggctt
    16441 tttccacaag cgcatcctct aagtgtaaat acatcaatgc agcgtcatgc cgcgtaatag
    16501 atttatgcta accagtcatt gccgtttacg ccacgttacg gacacttttt tacttttact
    16561 gcgaaggtga tcgaccaact gggattaggc ggggaaaaag gctacacttc gcctttgaaa
    16621 actcatcatc acgataaaca tagaggcgaa gtccaacgtg gctcaattcg tttataccat
    16681 gcatcgtgtc ggcaaagttg ttccgccgaa acgtcatatt ttgaaaaaca tctctctgag
    16741 tttcttccct ggggcaaaaa ttggtgtcct gggtctgaac ggcgcgggta agtctaccct
    16801 gctgcgcatt atggcgggca ttgataaaga catcgaaggt gaagcgcgtc cgcagccaga
    16861 catcaagatt ggttacctgc cgcaggaacc gcagctgaac ccggaacaca ccgtgcgtga
    16921 gtccattgaa gaagcggttt ctgaagtggt taacgccctg aaacgcctgg atgaagtgta
    16981 tgcgctgtac gccgatccgg atgccgattt tgacaagctg gccgctgaac aaggccgtct
    17041 ggaagagatc attcaggctc acgacggtca taacctgaac gtacagctgg agcgtgcggc
    17101 ggatgcgctg cgtctgccag attgggacgc gaaaatcgct aacctctccg gtggtgagcg
    17161 tcgtcgcgtg gcgttgtgcc gcctgcttct ggaaaaacca gacatgctgc tgctcgacga
    17221 accgaccaac cacctggatg ccgaatccgt ggcctggctg gaacgcttcc tgcacgactt
    17281 cgaaggcacc gttgtggcga ttactcacga ccgttacttc ctcgataacg ttgcaggctg
    17341 gatcctcgaa cttgaccgcg gtgaaggtat tccgtgggaa ggcaactact cctcctggct
    17401 ggagcagaaa gatcagcgcc tggcgcagga agcttcacaa gaagcggcgc gtcgtaagtc
    17461 gatcgagaaa gagctggagt gggtacgtca gggaactaaa ggccgccagt cgaaaggtaa
    17521 agcacgtctg gcacgctttg aagagctgaa cagcaccgaa tatcagaaac gtaacgaaac
    17581 caacgaactg tttattccac ctggaccgcg tctgggcgat aaagtgctgg aagtcagcaa
    17641 cctgcgtaaa tcctacggtg atcgcctgct gattgatgac ctgagcttct cgatcccgaa
    17701 aggggcaatc gtcgggatca tcggtccgaa cggcgcgggt aaatcgaccc tgttccgtat
    17761 gatctctggt caggaacagc cggacagcgg caccatcact ttaggtgaaa cggtgaaact
    17821 ggcatcggtt gatcagttcc gtgactcaat ggataacagc aaaaccgttt gggaagaagt
    17881 ttccggcggg ctggatatta tgaagatcgg caacaccgag atgccaagcc gcgcctacgt
    17941 tggccgcttt aactttaaag gggttgatca gggtaaacgc gttggtgaac tttccggtgg
    18001 tgagcgcggt cgtctgcatc tggcgaagct gctgcaggtt ggcggcaaca tgctgctgct
    18061 cgacgaacca accaacgacc tggatatcga aaccctgcgc gcgctggaaa acgccctgct
    18121 ggagttcccg ggctgtgcga tggttatctc gcacgaccgt tggttcctcg accgtatcgc
    18181 cacgcacatc ctggactacc aggatgaagg taaagttgag ttcttcgaag gtaactttac
    18241 tgagtacgaa gagtacaaga aacgcacgct gggcgcagac gcactggagc cgaagcgtat
    18301 caagtacaag cgtattgcga agtaatgcgt aaaatgccgg atgcggcgtg aacgccttat
    18361 ccggcctaca aaccaagaaa tttcaatacg ttgtgaatct ccaggcctga taagacgcgg
    18421 taagcgtcgc atcaggcatt gagcaccgaa tgccggatac ggcgtgaacg ccttttccgg
    18481 cctacaacag ccttagtatc cgcaaattcg tagcgggtca cccgtgctga ttcttttaaa
    18541 ccttgtttaa tctcgacagc ctcttccaga gaggttttca actcatcaaa aaacttattt
    18601 tatacctcgc ctttgagccg tttcatcgcg gttatctctg ctcccccatc atctcccgca
    18661 ccagctccac gcagcgcagg aaacggctgt cataatcgtc ctcttcaacc cgcacgaatt
    18721 cgatattgtt ctcttccagc atctccacca gcaagttctg gaactctttg cgatccaccg
    18781 aactgccgag gctgcgtaaa ccatccgcca cccacggcgt gttgttctcc agcaggatca
    18841 ccagatcgaa acggtattca tcaatcaacg cctgtacgaa cggatgctca cgcccttcgt
    18901 actttttgca gaacgcctgg gtagtgacaa aatcagtgtc aataaacgcc actttattgg
    18961 catatttcac tgcaaaatca atgtattgtg cgtggcccag cgcgatttta tcgtaatcgg
    19021 aatactgcaa tgcgatctca tcaccgccga ggtgtgaaaa gacataatcg cgaccatatt
    19081 cccacgcact ggtggtattg aagatattgg caagtttgtt taccagggtg gatttaccgc
    19141 tcgactcgcc accaaggatc gccacggtac gtacaaagaa cggcttcact tccgtaggaa
    19201 tatattccca gtagcggaac gggttttcgc ggatctgcgc accgctgata ctcataaagg
    19261 tacgtttcgg atcgaccagc accgtatcga tccccagatg ttccatatac tgtggcgcat
    19321 cggcttcttc cgaggtgtag atcaggtccg gctgaatccc cttttcagcc ataaactttt
    19381 tgatgccgtt gctccacaca tcccagccgt gcggatacgg ctccatgccc tcttcgttga
    19441 aagcatgaat gcgaatattt ttctgatatt taaaagtttg taataaccag cgcagacgat
    19501 ccggcaccgt gggctgctgc gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat
    19561 cgtcaaaacc cataatgata tgcagctcgt caacctggct acaggcgcgc tggataaggt
    19621 agatatgtcc ggtatgcagc gggtagaact taccgaatac gacaccaatg gttttcttct
    19681 gtcggggaaa ttcaagcccc aaaaaacggt gcaacgcctc cagcttttgc gcgctggggc
    19741 ttttaatttt ggcattcagt aactggctta aatacccttt ggtcataccg ctggcatcag
    19801 ctacctgctg tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg
    19861 acatatctcc ctccgtatct ctcattataa gtcgtcgaac acgctaagcg cgtcggagag
    19921 ttttttaacg ccaaaaatct gcatcccttc cggcgctttt ttcggtacgt tagccgccgg
    19981 aacaatcgcc cggcgaaaac cgtgtttcgc cgcttctgaa attcgttcct gaccgctggg
    20041 caccgggcgg atctcccctg ccagcccgac ttcaccaaac accaccagat cctgtggcag
    20101 cggtctgtca cgcaggctgg aaaccatcgc cagcagtaac gctaaatcgg cactggtttc
    20161 ggttaccttc acgccgccga ccacgttcac aaatacatcc tgatcggcca tttgcagacc
    20221 accgtgacgg tgcaagacag ccagcaggat tgccagacgg ttttgttcca gccccactgc
    20281 cacgcggcgc ggattcgcca tcatcgagtg atcgaccagc gcctgaatct ccaccagcaa
    20341 cggacgcgtt ccttcccaca ccaccatcac ggagctgccg gaggtcactt catctccgcg
    20401 acttaagaaa attgccgaag ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa
    20461 gacgcccagc tcattcaccg cgccgaagcg gtttttatgg ctgcgcaagg tgcgaaaacg
    20521 ggagtcggca tcaccatcca gaagcaccga acagtcgata cagtgttcca gcactttagg
    20581 gccagccagc gagccatctt tggttacgtg cccaaccatg acaatcgcca caccgcgcgt
    20641 tttggcgaag cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc taccaggcga
    20701 tgattgtaca tccgccatat gcatcacctg gatcgagtca attaccatca gtttcggttg
    20761 ctcttcttcg gcaatcaggc agatctgttc gatgctggtt tccgacaaca tattgagatt
    20821 gtcagtcggc aggccaaggc gatgagcgcg cattgccacc tgttgcagcg actcttcgcc
    20881 agtgacatac agcgttttca tctgctgggc cagtttgcac agcgtttgca gtagcagcgt
    20941 ggatttcccc gcacctgggt taccgccaat cagaatggcg cttcctggca ccacgccgcc
    21001 gcctagtacg cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct
    21061 gatatcggag agtttctgga cttttgccac cccggcgcta ccggcatagc cgctgagacg
    21121 ctcgttacgc gccaccgttg gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc
    21181 atgacaggca ctgcactgcc cctgccagcg cggataatcg gccccgcatt cattacaaac
    21241 aaaggcgcgt tttggagctt ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg
    21301 atggcgggcg agcaattact tctgattcag gctgccagag aggatgcaga atacccccat
    21361 caggtcagcg tgacggatgg tgacttccgc cttttcattc acttttggct tggcatggta
    21421 ggcaatcccc agccctgctg ctttgatcat cggcaggtca ttagctccat cgccaatcgc
    21481 cacggtctgc gccagcggga tttcatactc ctgcgcgagg cgagtcaaag ttttcgcttt
    21541 gtactgcgcg tctacgatgt cgccgatcac attgccggta aatttaccgt ccatgatctc
    21601 cagttcattg gctaccacgg cggtcaggcg cagcttgtcg cgcagatatt cagcaaagaa
    21661 agtaaagccg ccggaggcaa tcgccacttt ccagcccagc gtttccagct tgagcaccag
    21721 ttgcgttaag cctggcatca gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc
    21781 agcgcctttc agcgtcgcca cacggctgcg caggctggcg gtaaaatcga gttcgccgcg
    21841 catcgcccgt tcggttactt ccgccaccat ctcgcccgtt ccggccagtt tggcaatttc
    21901 atcaatacat tcaatctgga tggcggtgga atccatgtcc atcaccagca aacccggcgt
    21961 gcgcaggtgc gggattttcc ccagcggggc gacatccagt tgcgcttcat gggccaggcg
    22021 cgtagcccgt gcggtgagtg aacctgccag acgaatcacc tgataatctt ccacgcacca
    22081 ggcggcaaca atcaccatcg ccgcacccag tttgctctgg tattgggtca gacgctgttt
    22141 atccagccca cgaccataca gcagccagcc gctacgacct gcgtggtaat ccagtggcat
    22201 cacttcatca ccacttaatg aaagaggcag accaggccat aaagagacat cttcaggcag
    22261 gtcgcaccag gtaatgttag gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat
    22321 aacgcatgag gctaccttgt atccattgct tctggcaaca ttaagtctca aattttcaaa
    22381 gggtggaaga tggctcgcac aaaactgaaa ttccggctgc atcgggcagt gattgtcctg
    22441 ttctgtcttg ccttgttagt ggcgctgatg cagggagcgt catggtttag tcaaaaccac
    22501 cagcgacaac gtaatccaca gctggaagaa ctggcccgca ccctggcgcg tcaggtaacg
    22561 ctgaacgttg caccgctgat gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc
    22621 ctcgatcagt taacggatga aagccgtatc ctcgacgcgg gtgtgtatga cgaacaaggc
    22681 gatcttatcg cacgttctgg cgaaagcgtc gaagtgcgcg accggctggc gctcgacggt
    22741 aaaaaagcag gcggctattt taaccagcag attgtcgagc caattgcggg taaaaacgga
    22801 ccgctcggct atctgcgcct gacactcgac acccatacgc tcgccaccga agcccaacag
    22861 gtggataaca ccactaacat tttacgcctg atgttgctgc tctcactggc aatcggcgta
    22921 gtgctgaccc gcacgctgct acagggtaaa cgcacccgct ggcagcaatc gcccttcctg
    22981 ttaaccgcca gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt
    23041 actacaagaa aggaaatcgt tatgtccaca ttacgcctgc tcatttctga ctcttacgat
    23101 ccgtggttta acctggcggt ggaggaatgt atttttcgcc agatgcccgc cacgcagcgc
    23161 gttctgtttc tctggcgcaa tgccgacacg gtagtgattg gtcgcgcgca gaacccgtgg
    23221 aaagagtgta atacccggcg gatggaagaa gataacgtcc gcctggcgcg gcgcagtagc
    23281 ggtggcggcg cggtgttcca cgatctcggc aatacctgtt ttacctttat ggctggcaag
    23341 ccggagtacg ataaaaccat ctctacgtcg attgtgctca atgcgctgaa cgcgcttggc
    23401 gtcagcgccg aagcgtccgg acgtaacgat ctggtggtga aaaccgccga aggcgaccgc
    23461 aaagtctcag gctcggccta tcgcgaaacc aaagatcgtg gcttccacca cggcaccttg
    23521 ctgctcaatg ccgaccttag ccgcctggca aactatctca atccggataa aaagaaactg
    23581 gcggcgaaag gcattacctc agtgcgttcc cgcgtgacca acctcaccga gctgctgccg
    23641 gggatcaccc atgagcaggt ttgcgaggcc ataaccgagg cctttttcgc ccattatggc
    23701 gagcgcgtgg aagcggaaat catctccccg gacaaaacgc cagacttgcc aaacttcgcc
    23761 gaaacctttg cccgccagag tagctgggaa tggaacttcg gtcaggctcc ggcattctcg
    23821 catctgctgg atgaacgctt tacctggggc ggcgtggaac tgcatttcga cgttgaaaaa
    23881 ggccatatca cccgcgccca ggtgtttacc gacagcctca accccgcgcc gctggaagcc
    23941 ctcgccggac gactgcaagg ctgcctgtac cgcgcagata tgctgcaaca gaagtgcgaa
    24001 gcgctgttgg ttgacttccc ggaacaggaa aaagagctac gggagttatc gacgtggata
    24061 gcgggggcgg tgcggtaact cgctgattaa aatatccagg gaaggaaata tgatcgatct
    24121 ggaaaaccag gaacgggaga ttatcaattt aatgttcagc cagcgtattt cctggctagc
    24181 cgccgtgcgt atcaggcata aattatcgct tgctgaagta agtaaaatgt taggtatctc
    24241 gattaattct ctaaaataga tcgaaaagac ggaacggttt agctcaaaca tcaaaagcaa
    24301 aatggcagaa atttatggtt gtccaccaga gttgctgata tgcccttcct ggatgacggc
    24361 tgaacataaa tgaatagatt cgtgaataga tacttgaata gattttattt tctatccata
    24421 tgaaaaataa tagtaaatct ataaatcagt tgctattgat gaatagatac taaaaagccg
    24481 gagcagtctc ccactccggc ttcacacgac aatcgccttt cagcgaaaca caattactct
    24541 ttatcgccca gcagaacgga ttccagtgcg attttgatca tgtcgttgaa ggtggtctga
    24601 cgctcagcgg cagtggtctg ctcgtgagtg cggatgtggt cagacacggt gcagatggtc
    24661 agggctttcg cgccaaattc tgcagcgacg ccgtagatac cagcagcttc catttccacg
    24721 ccgaggatgc cgtatttttc catcacgtcg aacatttcgc cgtccggaga gtagaacagg
    24781 tcagcggaga acaggttacc aacgcgagca tcaacgccca gtgctttagc cgcgtctacc
    24841 gcgttacgca ccatgtcaaa gtcagcgata gcggcaaagt catggtcttt aaaacggata
    24901 cggttaactt tggaatcggt gcaggcaccc ataccgataa cgacgtcgcg cagttttacg
    24961 tgcggcagaa ctgcgccaca ggaacccacg cggataattt tcttcacgcc gaaatcggtg
    25021 atcagttctt tggtgtagat ggagcaggac gggataccca taccgtgacc cattacggaa
    25081 attttgcggc ctttgtaagt accggtgaag cccagcatac cgcgaacgtt gttcacttca
    25141 cgggcatctt caaggaaagt ttcagcaata tacttcgcac gcagcgggtc gcctggcatc
    25201 aaaactacgt cagcgaaatc gcccatttct gcattaatgt gtggagtagc cattgtttta
    25261 tccttatcaa catgttttca ccctctccct gtgggagagg gccggggtga gggcatcaag
    25321 ccgcacccat tatcagaaca tggctttgcc atattccata tcagaagtac caaaatattt
    25381 tgccagagtc tggccgatat ccgcgaaggt ttcacggtga cccagtgagc ccggttttac
    25441 tttcgggccg taaaccagta ccggaatgtg ttcacgcgtg tggtcagtac cggtccaggt
    25501 cggatcacaa ccgtggtcag cggtgaggat caggatgtcg tcatcgcgca gcagagacat
    25561 cagctccggc agacgacggt cgaacagctc cagacccgca gcataaccgg caacgtcgcg
    25621 acggtggccc caggaagagt cgaagtcaac gaagttggtg aagacgatgg tgttatcacc
    25681 cgcttctttc atctctttga tggtggcgtc aaacagcgcg tccaggccgg tcgctttcac
    25741 ttttttggtg ataccgcagt tggcgtagat gtcggcaatt ttaccgaccg aaaccacctg
    25801 gccgtgtttt tcatcaacca gtttctgcag cacggtcggt gctggcggtt caacagccag
    25861 gtcgtgacgg ttaccggtac gctggaagtt accggctttg tcaccgataa acggacgagc
    25921 gataacacga ccgatgttgt agccgccgtt ggtcagctct tcacgggcga tttcgcacag
    25981 ttcgtagagt ttatccagac cgaaagtttc ttcatggcag gcaatctgga acacggagtc
    26041 agcggaggta tagaaaatcg gcttgccggt tttcatgtgc tcttcgccca gttgatccag
    26101 aatgaccgta ccggaagagt ggcagttacc gaggtaaccc ggcagattag cgcgttcgac
    26161 cagtttatcc agcagctctt gcgggaagct gttttcgtga tcggagaaat atccccactc
    26221 aaacagaacc gggacaccgg cgatttccca gtgaccagac ggggtatctt taccggatga
    26281 catttcgtgc gcccatgcgt acgcgccgat aacttcagcg ttgccgtcca ttcccgccgg
    26341 aatgaaaccg gtagaacctt cgtgtgcttt cgccagcccc agacgggtca gatttggcag
    26401 attgagcggg cctttacgac cgttatcagc ttcgcctttg gcacaagctt ctgcgatatg
    26461 acccagggtg tcagccccga cgtcaccaaa gcgttctgca tcttctgtag cgccgatgcc
    26521 gaatgagtcc agcaccataa taaatgcacg tttcatatgt tctccgtact cagtgcttca
    26581 aatttaaatg atcagatcag tataccgtta ttcgctgata cggcgataga cagttggtgt
    26641 gctttccggt gctttatcgg caagtttaat tgccgctttc accgctttcg ccgcttcctg
    26701 ccagctgttt tcgtctttcg cgtggataac cgccagcgga cgctgaccgt ctacctggtc
    26761 gcccaaacgc gccatatcag taaagccgac gctgtaatcg atggtgtcag atgcctgacg
    26821 gcgtccgccg cccattgcaa ccactgccat ccccagcgcg cgggtatcca tttcactgac
    26881 aaaaccttcg gtatcagcat agactgcttt cgtcagcatc gctgtcggca gatacttcgc
    26941 gtagttctca acgaagtcgg tcgggccttt ttgtgccgct accatacgac caaagacttc
    27001 tgccgcttta ccgttgtcca gcaccgcctg caatttcgcg cgcgcttcgg cgtcatcttt
    27061 cgccagtttg ccggagatca gcatctccac gcacagtgcc atcgtgacat caaacagacg
    27121 cgggttacga tattcacccg tcaggaactg caccgcttca cgaacttcaa ccgcgttacc
    27181 tgcactggag gccagtacct gattcatgtc ggtgagcagc gcggtggtgc gcacgccagc
    27241 gccgttagcc acgccaacaa tcgcttcggc aagggcttca gagagttcgt aggtcggcat
    27301 aaacgcgccg ctacccactt tcacgtccat caccagcgca tccagacctt ccgccagttt
    27361 cttcgccagg atcgaggcag tgatcagcgg gatggagtcc acggttgcgg taatatcgcg
    27421 ggtcgcgtag aaacgtttat ctgccggagc caatgagctg gtctggccga taatcgccac
    27481 gccgacgtct ttaataattt cgcggaaacg gttgtcatcc gggaaaatgt cgaagccagg
    27541 gatggattcc agtttgtcga gcgtaccgcc agtatgaccg aggccgcgac cagagatcat
    27601 cggaatatag ccgccgcagg ctgcgaccat cggccccaac atcagcgaag tcacatcgcc
    27661 gacgccgccg gtcgagtgtt tatcaacaat cgggccattc agatgcaggc ttttccagtc
    27721 gagaacggtt cctgaatctc gcatcgccat ggtcagcgag acacgctcag gcattgtcat
    27781 atcgtggaag aaaatggtca tcgcgagggc ggcaatctgc ccttcggaga tagtgttgtc
    27841 gcgaataccg ttgataaaga aacgaatttc ttcatcgctc agcgcatgac catcacgttt
    27901 tttacgaata atttcttgtg cgagaaacaa ggtaacctcc cgaggaaaag agtatgaggc
    27961 ggcttttgcc ggatgcgtct acgccttatc cggcctacgg gtagatacgt ctcgtaggcc
    28021 tgataagcgc agcgcatcag gcgtaaagca tcttacttag tagctgctgg cgctcttacc
    28081 gtcgccgtgg cccagagcct tcagcaggct tgccagcagg ctggaagcac caaagcggta
    28141 gtggcgcgca tcggcccagt cagcgccgaa cagttcatct gcgatggcga gatatttctg
    28201 cgcatcttcc gcagtacgca cgccgcccgc cggtttgaaa ccaacggttt tttctacgcc
    28261 catatcacga atcacttcca tcatgatgcg cgcgctttcc ggcgtcgcgt tcactgccac
    28321 tttaccggta gaggttttga tgaagtcagc acccgctttg atggagattt cagacgcttt
    28381 acggatcagc gcttcgtctt tcagttcgcc ggtttcgatg atcactttca gcagtacatt
    28441 cgctgccgcg caagcctctt tacaggcttt caccaggtca aaaccaacct gctcgttacc
    28501 cgccatcagc gcacggtacg ggaacaccac gtcaacttca tcggcaccgt aggcgattgc
    28561 cgcacgggtt tctgccagcg cgatttcgat gtcgtcgtta ccgtgtggga agttagttac
    28621 cgtagcaata cggatttccg gggtgccctg ctctttcagc gttttgcgag caatcgggat
    28681 aaagcgagga tagatacaga tagcggcggt attgccgacc ggagttttgg cctgatgaca
    28741 cagagcaatt actttctcgt cggtgtcgtc gtcattcagg gtggtcaggt ccatcaattt
    28801 cagtgcacgc aggctgcttg ctttcagatc agtcatttca ttctcctggc ttgtcgccaa
    28861 taaaattcac cttgcgagtt tgttagtatt ctaacatcta ctccgcaaca cacttcgata
    28921 tacatcacaa ttaaggaaat ctacttacaa gtttgcatca ctgtaatgcg atctggttca
    28981 aataattcac tttcaaatga atgcgtcagt ggtggcaaac gcatcaggat caaagtgaac
    29041 atcacgaaac ttcttacaat ggcgcatcac cttcggcata aaaggaaaag aagatgccca
    29101 cttctcatga aaatgcactg caacaacgtt gccagcaaat tgtcaccagc ccagtgctta
    29161 gcccggagca gaagcgccat tttctggctc tggaagcaga aaacaatctg ccttatccgc
    29221 agcttcctgc cgaagcccgc cgcgcgctgg atgagggtgt aatctgcgat atgtttgaag
    29281 gtcatgcgcc gtacaaaccg cgctatgtct tacccgatta cgcccgtttt ctggcgaacg
    29341 gttccgaatg gctggagctg gaaggcgcga aagatcttga tgacgcactc tctctgctga
    29401 ccattcttta ccaccacgta ccgtcggtca catcgatgcc ggtctacctg gggcaactgg
    29461 atgcgttgtt gcaaccgtat gttagaattc taacacaaga cgagatcgat attcgaataa
    29521 aacgtttctg gcgttacctc gacagaaccc tgccagacgc ctttatgcac gccaatatcg
    29581 gcccgtctga ttcgcccatt acccgcgcga tcttacgtgc agacgcagag ttgaagcagg
    29641 tttcaccgaa cctgaccttt atctacgatc ctgaaatcac ccctgatgac ctgctgctgg
    29701 aagtggcgaa gaacatctgt gaatgtagca aaccgcacat cgccaacggt ccggtgcatg
    29761 ataaaatttt cacaaaaggg ggctacggga ttgtgagctg ttacaactca ctgccgctgg
    29821 cgggtggtgg cagcacgctg gtacgcctta acctgaaagc cattgccgag cgcagcgaat
    29881 cgctggatga cttctttacg cgcactctac cgcactactg ccagcagcag atcgccatca
    29941 tcgatgcgcg gtgtgaattc ctctatcaac aatcacactt ctttgagaat agcttcctgg
    30001 tgaaagaagg gctgattaac cctgaacgtt ttgtgccaat gtttggcatg tatgggctgg
    30061 cggaagcggt taacttgctg tgtgagaaag aagggattgc cgcacgttac ggtaaagaag
    30121 ccgccgcaaa tgaagtgggt tatcgcatca gcgcgcaact ggcggagttt gtcgccaata
    30181 cccccgtgaa atatggatgg caaaaacgcg ccatgttaca cgcacagtcg gggatcagtt
    30241 ccgatatcgg caccacgccg ggcgcgcgtt tgccgtatgg cgatgagcca gatccgatca
    30301 cccatctgca aactgtcgcc ccgcatcatg cttattatta ttccggcatc agcgacattc
    30361 tgacgctcga cgaaaccatc aaacgtaacc cgcaggcact ggtacagctt tgcctcggtg
    30421 cctttaaagc cggaatgcgt gaatttaccg ccaatgtcag cggtaacgat ctggttcgcg
    30481 ttaccggtta tatggtgcgt ttgtcggatt tagaaaaata tcgcgccgaa ggttcacgca
    30541 ccaacaccac ctggctgggc gaagaagccg cacgcaacac tcgtattctg gaacgccagc
    30601 cgcgcgtgat aagccatgaa cagcagatgc gctttagtca gtaagattat ccccttctcc
    30661 tgcgttgacg ggccaggcag tcgtctggct ctgtttttgc agggctgcaa tctgcgctgc
    30721 aaaaactgtc acaatccgtg gacgatggga cgttgcaatg actgtgggga gtgcgtgcca
    30781 cagtgtccgc atcaggcgtt gcagattgtt gacggcaaag tggtgtggaa cgctgtggtc
    30841 tgcgagcaat gtgatacctg cctgaagagg tgtccgcaac atgccacgcc aatggcgcaa
    30901 tccatgagcg tggatgaggt gcttagccat atccgcaaag cggtgctgtt tattgaaggg
    30961 ataacggtaa gcggcggcga agccacgacc caactgccgt ttgtagtggc gctgtttact
    31021 gctatcaaaa acgatccgca actgcgccac ctcacctgcc tggtggacag taacggtatg
    31081 ttgagcgaaa ccggctggga aaaattactc ccggtgtgcg acggcgcaat gctcgatctc
    31141 aaagcgtggg ggagcgaatg tcatcagcaa ttaaccggac gcgataatca gcagattaag
    31201 cgcagcatct gtttgctggc agagcgcggt aagctggcgg aactgcgttt gctggtgatc
    31261 cctggccagg tggattattt gcaacacatc gaagaactgg cggcgtttat caagggactc
    31321 ggcgatgttc cggtacgcct gaacgcgttt catgcccacg gcgtgtatgg cgaggcgcaa
    31381 agctgggcga gcgccacgcc ggaagacgtt gagccgttgg ctgatgcgtt aaaggtgcgc
    31441 ggggtgagcc ggttgatatt tccggcgctc tatttgtgac atgccggata cggcctacgg
    31501 ttcggcacag ac
//
LOCUS       DAZGSP010000059        31447 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZGSP010000059 DAZGSP010000000
VERSION     DAZGSP010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.059"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(39..1403)
                     /gene="rhlE"
                     /locus_tag="ACHK74_002902"
     CDS             complement(39..1403)
                     /gene="rhlE"
                     /locus_tag="ACHK74_002902"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFP0221680.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRRGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            1632..2303
                     /gene="cecR"
                     /locus_tag="ACHK74_002903"
     CDS             1632..2303
                     /gene="cecR"
                     /locus_tag="ACHK74_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFP0221681.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2306..3301
                     /gene="hlyD"
                     /locus_tag="ACHK74_002904"
     CDS             2306..3301
                     /gene="hlyD"
                     /locus_tag="ACHK74_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFP0221682.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYNLMLA
                     GYRDEEIAQATAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            3294..5030
                     /gene="ybhF"
                     /locus_tag="ACHK74_002905"
     CDS             3294..5030
                     /gene="ybhF"
                     /locus_tag="ACHK74_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP0221683.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5023..6156
                     /gene="ybhS"
                     /locus_tag="ACHK74_002906"
     CDS             5023..6156
                     /gene="ybhS"
                     /locus_tag="ACHK74_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0221684.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6167..7273
                     /gene="ybhR"
                     /locus_tag="ACHK74_002907"
     CDS             6167..7273
                     /gene="ybhR"
                     /locus_tag="ACHK74_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0221685.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7235..7645)
                     /gene="ybhQ"
                     /locus_tag="ACHK74_002908"
     CDS             complement(7235..7645)
                     /gene="ybhQ"
                     /locus_tag="ACHK74_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFP0221686.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7778..8539
                     /gene="ybhP"
                     /locus_tag="ACHK74_002909"
     CDS             7778..8539
                     /gene="ybhP"
                     /locus_tag="ACHK74_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP0221687.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8536..9777
                     /gene="clsB"
                     /locus_tag="ACHK74_002910"
     CDS             8536..9777
                     /gene="clsB"
                     /locus_tag="ACHK74_002910"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFP0221688.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            9777..10733
                     /gene="ybhN"
                     /locus_tag="ACHK74_002911"
     CDS             9777..10733
                     /gene="ybhN"
                     /locus_tag="ACHK74_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFP0221689.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10769..11482)
                     /gene="ybhM"
                     /locus_tag="ACHK74_002912"
     CDS             complement(10769..11482)
                     /gene="ybhM"
                     /locus_tag="ACHK74_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP0221690.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(11687..12391)
                     /gene="ybhL"
                     /locus_tag="ACHK74_002913"
     CDS             complement(11687..12391)
                     /gene="ybhL"
                     /locus_tag="ACHK74_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP0221691.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGITTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(12528..12980)
                     /gene="moaE"
                     /locus_tag="ACHK74_002914"
     CDS             complement(12528..12980)
                     /gene="moaE"
                     /locus_tag="ACHK74_002914"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFP0221692.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(12982..13227)
                     /gene="moaD"
                     /locus_tag="ACHK74_002915"
     CDS             complement(12982..13227)
                     /gene="moaD"
                     /locus_tag="ACHK74_002915"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFP0221693.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(13220..13705)
                     /gene="moaC"
                     /locus_tag="ACHK74_002916"
     CDS             complement(13220..13705)
                     /gene="moaC"
                     /locus_tag="ACHK74_002916"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFP0221694.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(13708..14220)
                     /gene="moaB"
                     /locus_tag="ACHK74_002917"
     CDS             complement(13708..14220)
                     /gene="moaB"
                     /locus_tag="ACHK74_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFP0221695.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(14242..15231)
                     /gene="moaA"
                     /locus_tag="ACHK74_002918"
     CDS             complement(14242..15231)
                     /gene="moaA"
                     /locus_tag="ACHK74_002918"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFP0221696.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(15219..15363)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            15628..16536
                     /gene="yvcK"
                     /locus_tag="ACHK74_002919"
     CDS             15628..16536
                     /gene="yvcK"
                     /locus_tag="ACHK74_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFP0221697.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(16728..18749)
                     /gene="uvrB"
                     /locus_tag="ACHK74_002920"
     CDS             complement(16728..18749)
                     /gene="uvrB"
                     /locus_tag="ACHK74_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP0221698.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(19328..20005)
                     /gene="bioD"
                     /locus_tag="ACHK74_002921"
     CDS             complement(19328..20005)
                     /gene="bioD"
                     /locus_tag="ACHK74_002921"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP0221699.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(19998..20753)
                     /gene="bioC"
                     /locus_tag="ACHK74_002922"
     CDS             complement(19998..20753)
                     /gene="bioC"
                     /locus_tag="ACHK74_002922"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFP0221700.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPGAIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQKQGRYPLTYHLFLGVIARE"
     gene            complement(20740..21894)
                     /gene="bioF"
                     /locus_tag="ACHK74_002923"
     CDS             complement(20740..21894)
                     /gene="bioF"
                     /locus_tag="ACHK74_002923"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFP0221701.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIAADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQMVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSEEGDARREKLAALITRFRAGVQGSPFTLADSRSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEMLHGNG"
     gene            complement(21891..22931)
                     /gene="bioB"
                     /locus_tag="ACHK74_002924"
     CDS             complement(21891..22931)
                     /gene="bioB"
                     /locus_tag="ACHK74_002924"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFP0221702.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            23018..24307
                     /gene="bioA"
                     /locus_tag="ACHK74_002925"
     CDS             23018..24307
                     /gene="bioA"
                     /locus_tag="ACHK74_002925"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFP0221703.1"
                     /translation="MTTDDIAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            24366..24842
                     /gene="ybhB"
                     /locus_tag="ACHK74_002926"
     CDS             24366..24842
                     /gene="ybhB"
                     /locus_tag="ACHK74_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFP0221704.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDEVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            24994..26277
                     /gene="ybhC"
                     /locus_tag="ACHK74_002927"
     CDS             24994..26277
                     /gene="ybhC"
                     /locus_tag="ACHK74_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFP0221705.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(26419..28680)
                     /gene="ybhJ"
                     /locus_tag="ACHK74_002928"
     CDS             complement(26419..28680)
                     /gene="ybhJ"
                     /locus_tag="ACHK74_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFP0221706.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFIDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(28863..30296)
                     /gene="ybhI"
                     /locus_tag="ACHK74_002929"
     CDS             complement(28863..30296)
                     /gene="ybhI"
                     /locus_tag="ACHK74_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFP0221707.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(30372..31424)
                     /gene="ybhH"
                     /locus_tag="ACHK74_002930"
     CDS             complement(30372..31424)
                     /gene="ybhH"
                     /locus_tag="ACHK74_002930"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFP0221708.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISLAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
BASE COUNT         7435 a         8419 c         8124 g         7469 t
ORIGIN      
        1 tcatcagcct gatgccgggc atagcccggc ataaaagatt actgcgcagc ggtaggttta
       61 cgcggacggc ggcgacgttg ttgttcgcct gccggtttgg catcgccgcg gcggcgagac
      121 ggtttttctg caggtttcgc gcttgcagat tttgcgccac cttccccacg gcgtggttgc
      181 tgttgaccgc gaccaccacc ttgcccacga ccgccgccgc cacgttgctg gcgaccgttc
      241 tggatcggtt cggctttgat tgacgggtcc ggctcatagc ccggaatcgc aatgcgcggg
      301 atctcttttt tcagcagttt ttcgatatca cgcagcagtt tgtgttcatc aacacacacc
      361 agcgacaacg cttcaccggt agcagccgca cgaccggtac gcccgatacg gtggacataa
      421 tcttccggta cgtttggcag ttcatagttg acgacgtgcg gcaactcttc gatatccaga
      481 ccgcgcgcag cgatatcggt cgccaccagt acgcgaatgt cacccgattt aaaatcagcc
      541 agcgcacggg tacgcgcacc ttgcgattta ttcccgtgga tcgccgcact acggatgcca
      601 tctttattga gctgctcagc cagatggtta gcgccgtgtt tggtacgggt aaacaccagc
      661 acctgctgcc agttcccttt accaatcatg tgcgacagca attcgcgttt gcgtttctta
      721 tcaacaaagt gaacgtgctg ggtcacctga tcagacgcgg tattgcggcg tgccacttcg
      781 atttccagcg ggttgtgcaa cagtttttcc gccagggctt taatatcgtc agagaaggtc
      841 gcagagaaca gcaggttctg gcgtttcgct ggcagttttg ttaacacgcg acggatatcg
      901 tggataaagc ccatgtcgag catgcggtca gcttcatcga ggacgaggat ttcaacctga
      961 tccagcttca ctgcattttg atgttccagg tccagcaaac gtcccggtgt tgccaccaga
     1021 acatcaacgc cgccgcgcag cttcatcatt tgcgggttaa tgctgacacc gccaaacacc
     1081 accagcgaac gaatgttcaa atatttgctg taatcacgga cgttttcgcc aatctgcgca
     1141 gccagttcac gggtcggggt aagaatgagc gcacgtaccg gacgacgccc tttggcgtgc
     1201 ggctggcgag tgatcaggtg ttgcaacagc ggcagcgtaa agcccgctgt tttaccggta
     1261 ccggtctggg cgctagccat caggtcgcgg ccttccagca ccgcagggat cgcctgctgc
     1321 tgaatagggg tgggttcacg gtaaccctgc tcggcaacgg cgcgcaggat atcagggctt
     1381 aaacccaaag aatcgaaaga cataactact ccgaaccgcc ccgaccgtta ccggtgtagt
     1441 tttcagggag atactgaaaa ggaaaatgac aaaaaccaca atgtcatgag ggagcggagt
     1501 gtagcagttt ttgtgacgca gcgcataaat tatccctgtg actggcgtag ctaaaattct
     1561 tagccatact ggacaactcc catatggagt cataatctta atcaatcatt tgattaagaa
     1621 tggactgtgc gatgaataat cctgccatga caatcaaggg tgaacaggcg aaaaaacagc
     1681 tgattgctgc cgcactggcg cagtttggtg aatatggaat gaacgccacc acccgcgaga
     1741 tagccgccca ggccgggcag aatatcgctg ccatcaccta ctacttcggt tcgaaagaag
     1801 atttgtacct cgcctgcgcc cagtggattg ccgattttat tggcgagcag ttccgtccgc
     1861 acgccgaaga ggccgaacgc ttgttcgcac aaccacagcc tgatcgcgca gccatccgtg
     1921 aactgatcct tcgcgcctgc aggaacatga ttaagctgct cacccaggat gacaccgtca
     1981 acctcagcaa gtttatctcc cgtgagcagc tctctcccac ggcagcctat cacctggtgc
     2041 acgaacaggt gattagcccg ctacacagcc acctgacacg cctgattgcc gcctggaccg
     2101 gctgcgacgc caacgatacc cgcatgatcc tccatactca tgcgttgatt ggtgagattc
     2161 tggcgttccg tcttggcaaa gaaacaattc tgttacgtac cggctggacc gcgttcgatg
     2221 aagaaaagac cgaactgatc aaccagacgg tgacctgtca tatcgacctg attttgcaag
     2281 gattatcgca aaggagtttg tagtgatgaa aaaacctgtc gtgatcggac tggcggtagt
     2341 ggtacttgcc gccgtggttg ccggaggcta ctggtggtat caaagccgcc aggataacgg
     2401 cctgacgctg tatggcaacg tggatattcg tacggtgaat cttagtttcc gtgttggggg
     2461 gcgcgttgaa tcgctggcgg tggacgaagg tgatgctatc aaagcgggcc aggtgctggg
     2521 cgaactggat cacaagccgt atgagattgc cctgatgcag gcgaaagcgg gtgtttcggt
     2581 ggcacaggcg cagtataacc tgatgcttgc cgggtatcgc gatgaagaaa tcgctcaggc
     2641 caccgcagcg gtgaaacagg cgcaagccgc ctatgactat gcgcagaact tctataaccg
     2701 ccagcaaggg ttgtggaaaa gccgcaccat ttcggcaaac gacctggaaa atgcccgctc
     2761 ctcgcgcgac caggcgcagg caacgctgaa atcagcacag gataaattgc gtcagtaccg
     2821 ttccggtaac cgtgaacagg acatcgccca ggcgaaagcc agcctcgaac aggcgcaggc
     2881 gcaactggcg caggcggagt tgaatttgca ggactcaacg ttgatagccc cgtctgatgg
     2941 cacgctgtta acgcgcgcgg tggagccagg cacggtcctc aatgaaggtg gcacggtgtt
     3001 taccgtttca ctaacgcgtc cggtgtgggt acgtgcctat gttgacgaac gtaatcttga
     3061 ccaggcccag ccggggcgca aagtgctgct ttataccgat ggtcgcccgg acaagcccta
     3121 tcacgggcag attggtttcg tttcgccgac tgctgaattt accccgaaaa ccgtcgaaac
     3181 accggatctg cgtaccgacc tcgtctatcg cctgcgtatt gtggtgaccg acgccgatga
     3241 tgcgttacgc cagggaatgc cggtgacggt gcaattcggt aacgaggcag gacatgaatg
     3301 atgccgttat cacgctgaac ggcctggaaa aacgctttcc gggcatggac aagcccgccg
     3361 tcgcgccgct cgattgtacc attcacgccg gttatgtgac ggggctggtg gggccggatg
     3421 gtgcaggtaa aaccacgcta atgcggatgc tggcgggatt actgaaaccc gacagcggca
     3481 atgccacggt gattggcttt gatccaatca aaaacgacgg cgcgctgcac gccgtgctcg
     3541 gctatatgcc gcagaaattt ggtctgtatg aagatctcac ggtgatggag aacctcaatc
     3601 tgtacgcgga tttgcgcagc gtcaccggcg aggcacgtaa gcaaactttt gctcgcctgc
     3661 tggagtttac gtctcttggg ccgtttaccg gacgcctggc gggcaagctc tccggtggga
     3721 tgaaacaaaa actcggtctg gcctgtaccc tggtgggcga accgaaagtg ttgctgctcg
     3781 atgaacccgg cgtcggcgtt gaccctatct cacggcgcga actgtggcag atggtgcatg
     3841 agctggcagg cgaagggatg ttaatcctct ggagtacctc gtatctcgac gaagccgagc
     3901 agtgccgtga cgtgttactg atgaacgaag gcgagttgct gtatcagggg gaaccaaaag
     3961 ccctgacaca aaccatggcc ggacgcagtt ttctgatgac cagtccacac gagggcaacc
     4021 gcaaactatt gcaacgcgcc ttgaaactgc cacaggtcag cgacggcatg attcagggga
     4081 aatcggtacg tctgatcctc aaaaaagagg ccacaccaga cgatattcgc catgccgacg
     4141 ggatgccgga aatcaacatc aacgaaacta cgccgcgttt tgaagatgca tttattgatt
     4201 tgctgggtgg tgccggaacc tcagaatcgc cgctgggcgc aatattacat acggtagaag
     4261 gcacacccgg cgagacggtg atcgaagcga aagaactgac caagaaattt ggtgattttg
     4321 ccgccaccga tcacgtcaac tttgccgtta aacgtgggga gatttttggt ttgctggggc
     4381 caaacggcgc gggtaaatcg accaccttta agatgatgtg cggtttgctg gtgccgactt
     4441 ccggccaggc gctggtgctg gggatggatc tgaaagagag ttctggtaaa gcgcgccagc
     4501 atctcggcta tatggcgcaa aagttttcgc tctacggtaa cctgacggtc gaacagaatt
     4561 tacgcttttt ctctggtgtg tatggcttac gcggtcgggc gcagaacgaa aaaatctccc
     4621 gtatgagcga ggcgttcggc ctgaaaagta tcgcctccca cgccaccgat gaactgccat
     4681 taggttttaa acagcggctg gcactggcct gctcgctgat gcatgaaccg gacattctgt
     4741 ttctcgacga accgacgtcc ggcgttgatc cgattactcg ccgcgaattc tggctacata
     4801 tcaacagcat ggtagagaaa ggcgtcacgg tgatggtcac cacccacttt atggatgaag
     4861 cagaatattg cgaccgcatc ggcctggtgt accgtgggaa attaattgcc agcggcacgc
     4921 cggatgattt gaaagcgcag tcggccaacg acgagcaacc cgatcccacc atggagcaag
     4981 cctttattca gttgatccac gactgggata aggagcatag caatgagtaa cccgatcctt
     5041 tcctggcgtc gcgtgcgggc gctgtgcgtc aaagagacgc ggcagatcgt tcgcgatccg
     5101 agtagctggc tgattgcggt agtgatcccg ctgttgctgc tgtttatttt tggttacggc
     5161 attaacctcg actccagcaa gctgcgggtc gggattttac tggaacagcg tagcgaagcg
     5221 gcgctggatt tcacccacac catgaccggt tcgccctaca tcgacgccac catcagcgat
     5281 aaccgtcagg aactgatcgc caaaatgcag gcggggaaaa ttcgcggtct ggtggttatt
     5341 ccggtggatt ttgcggaaca gatggagcgc gccaacgcca ccgcaccgat tcaggtgatc
     5401 accgacggca gtgagccgaa taccgctaac tttgtacagg ggtatgtcga agggatctgg
     5461 cagatctggc aaatgcagcg agcggaggac aacgggcaga cttttgaacc gcttattgat
     5521 gtacaaaccc gctactggtt taacccggcg gcgattagcc agcacttcat tatccccggt
     5581 gcggtgacca ttatcatgac ggtcatcggc gcgattctca cctcgctggt ggtggcgcga
     5641 gaatgggaac gcggcaccat ggaggctctg ctctctacgg agattacccg cacggaactg
     5701 ctgctgtgta agctgatccc ttattacttt ctcgggatgc tggcgatgtt gctgtgtatg
     5761 ctggtgtcag tgtttattct cggcgtgccg tatcgcgggt cgctgctgat tctgtttttt
     5821 atctccagcc tgtttttact cagtaccctg gggatggggc tgctgatttc cacgattacc
     5881 cgcaaccagt tcaatgccgc tcaggtcgcc ctgaacgccg cttttctgcc gtcgattatg
     5941 ctttccggct ttatttttca gatcgacagt atgcccgcgg tgatccgcgc ggtgacgtac
     6001 attattcccg ctcgttattt cgtcagcacc ctgcaaagcc tgttcctcgc cgggaatatt
     6061 ccagtggtgc tggtggtaaa cgtgctgttt ttgatcgctt cggcggtgat gtttatcggc
     6121 ctgacgtggc tgaaaaccaa acgtcggctg gattagggag aagagcatgt ttcatcgctt
     6181 atggacgtta atccgcaaag agttgcagtc gctgctgcgc gaaccgcaaa cccgcgcgat
     6241 tctgatttta cctgtgctta ttcaggtgat tctgttcccg ttcgccgcca cgctggaagt
     6301 gactaacgcc accatcgcca tctacgatga agataacggc gagcattcgg tggagctgac
     6361 ccaacgtttt gcccgcgcca gcgcctttac tcatgtgctg ctgctgaaaa gcccacagga
     6421 gatccgccca accatcgaca cacaaaaggc gttactactg gtgcgtttcc cggctgactt
     6481 ctcgcgcaaa ctggatacct tccagaccgc gccgttgcag ttgatcctcg acgggcgtaa
     6541 ctccaacagt gcgcaaattg ccgccaacta cctgcaacag atcgtcaaaa attatcagca
     6601 ggagctgctg gaaggaaaac cgaaacctaa caacagcgag ctggtggtac gcaactggta
     6661 taacccgaat cttgactaca aatggtttgt ggtgccgtcg ctgatcgcca tgatcaccac
     6721 tatcggcgta atgatcgtca cttcactttc cgtcgcccgc gaacgtgaac aaggtacgct
     6781 cgatcagcta ctggtttcgc cgctcaccac ctggcagatc ttcatcggca aagccgtacc
     6841 ggcgttaatt gtcgccacct tccaggccac cattgtgctg gcgattggta tctgggcgta
     6901 tcaaatcccc ttcgccggat cgctggcgct gttctacttt acgatggtga tttacggttt
     6961 atcgctggtg ggattcggtc tgttgatttc atcactctgc tcgacacaac agcaggcgtt
     7021 tatcggcgtg tttgttttta tgatgcccgc cattctcctt tccggttacg tttctccggt
     7081 ggaaaacatg ccagtatggc tgcaaaacct gacgtggatt aaccctattc gccactttac
     7141 ggacattacc aagcagattt atttgaagga tgcgagtctg gatattgtgt ggaatagttt
     7201 gtggccgcta ctggtgataa cggccacgac agggtcagcg gcgtacgcga tgtttagacg
     7261 taaggtgatg taacttctta tctttcgcca gcaaagacac taccgccggg ccggcaagga
     7321 ttgccagccc tgcaattgcc agcaaaaagt tgttttccag tacacgggac aacagccaca
     7381 gcacaatcag cgctgcgcca aaataccagg tcgtggtcag ctcttccagc acgtctgcca
     7441 cctcacgcca gcgttgctcg gggtggcgct gaaacaccaa catcatcact accgtgacac
     7501 aatacagtgc gcataatccc acgccgacgt gaaccagagt cttactcatc cagcccacca
     7561 ccagcagcgc cactaccagt aaatgcaaca taatctgcca gcaacttaga ccagttgcga
     7621 cacgaacacg ttgttgccac ttcatggctt ctctcctgaa gaatttatct ctacttactc
     7681 agagtaccga actttacgga aaattccgta agaccgcacc tttttgtgac gccgactaca
     7741 ctatttcaat gagataagag gcaaagacag gaaaattatg cccgatcaaa cacaacaatt
     7801 ttcgttcaag gtgctcacca tcaacattca caaaggtttt accgcgttta accgacgctt
     7861 cattttgccg gaacttcgcg acgcggtgcg taccgtcagc gccgatattg tttgcctgca
     7921 ggaagtgatg ggcgcgcacg aagttcatcc gctgcatgtg gaaaactggc ccgatacctc
     7981 gcactacgag tttctcgccg acacgatgtg gagcgatttt gcctacggtc gcaatgccgt
     8041 atacccggaa gggcatcacg gcaacgccgt actgtcgcgt tatcccattg aacattatga
     8101 gaatcgcgat gtttcggtcg atggtgcgga aaagcgcggc gtgctctact gccgcattgt
     8161 gccaccgatg accggaaaag cgattcatgt gatgtgcgta catctgggcc tgcgtgaggc
     8221 gcaccgtcag gcgcagcttg cgatgctcgc cgaatgggtg aatgagctac cggacggcga
     8281 accggtattg gtggcgggtg atttcaatga ctggcggcaa aaagctaatc atccgttaaa
     8341 agtgcaggcc ggactggatg agatttttac ccgcgcccac ggacgcccgg cgcgcacgtt
     8401 tccggtgcaa tttcctctac tacgactgga caggatctac gtcaaaaatg ccagcgccag
     8461 cgcgccaacc gcgttgccgc tgcggacatg gcgacacctt tctgatcatg cccctttaag
     8521 tgcggagatt catttatgaa atgtagctgg cgcgaaggca ataagatcca gttgctggaa
     8581 aacggcgagc aatattatcc cgcggtgttt aaggcgattg gcgaggcaca agaacgcatc
     8641 attcttgaaa cgtttatctg gtttgaggat gacgtcggca aacagctgca tgcggcacta
     8701 ctggcagcag cgcaacgcgg ggttaaagcg gaagtcttgc tggatggcta cggttcgccg
     8761 gatctcagcg atgagtttgt caatgaactg acggcagctg gcgtggtatt tcgctactac
     8821 gatccccgcc ctcgcctttt tggtatgcgc accaatgtgt ttcgccggat gcatcgcaaa
     8881 attgtggtga tcgacgcgcg tatcgccttt attggcgggc tgaattactc cgccgagcat
     8941 atgtccagct acggtccaga ggctaaacag gattacgcgg tacgccttga agggccgatt
     9001 gtcgaagata ttctccagtt tgagctggaa aacctgcctg gacagagcgc ggcacgacgc
     9061 tggtggcgac gtcatcacaa agcggaagag aaccgccagc cgggagaagc gcaggtattg
     9121 ctggtctggc gcgataacga agaacatcgc gatgatattg aacgtcacta tctgaaaatg
     9181 ctcactcagg cgcggcgaga agtcattatc gccaacgcct acttcttccc cggctatcga
     9241 tttttacacg ccttgcgtaa agcggcacgg cgcggggtgc ggatcaaact gatcattcag
     9301 ggcgaaccgg atatgccgat tgtcagagtc ggtgcgcgct tgctgtataa ctatctggtt
     9361 aaaggcggcg ttcaggtatt tgagtaccgc cgccgcccgc tccacggcaa agtggcattg
     9421 atggacgatc actgggcgac agtagggtcc agtaatctcg atccgctcag tttgtcactg
     9481 aatctcgaag caaatgtcat catccacgat cgtcatttta accagacgct gcgcgataat
     9541 ctgaacggca ttattgccgc agattgtcag caggtcgatg agaccatgct gccgaaacgc
     9601 acctggtgga acctgaccaa aagcgtgctg gcgttccact ttttacgcca cttcccggcg
     9661 ctggttggct ggcttccggc acacacacca cgtctggcgc aggttgatcc gcccgcacaa
     9721 ccgacaatgg aaacgcagga tcgggtagaa actgaaaaca cgggggtaaa accctgatga
     9781 gtaaatcaca cccgcgctgg cgcttagcaa agaagatcct cacctggctg ttttttatcg
     9841 cggtgattgt gttgctggtg gtctacgcca aaaaagtgga ctgggaagag gtctggaagg
     9901 tcatccgcga ctacaatcgc gttgcgctgc ttagtgcggt cgggctggtg gtcgtcagct
     9961 atctgattta cggctgctat gacctgctcg cccgctttta ctgcggtcac aaactggcga
    10021 agcgccaggt gatgctggtg tcgtttatct gctacgcctt caacctgacg ctcagtacct
    10081 gggtcggcgg cattggtatg cgctatcgtt tgtactcccg actagggtta ccgggcagca
    10141 ctattacgcg gattttctcg ctcagtatta ccaccaactg gctgggctat attttactgg
    10201 cagggattat ctttaccgca ggcgtggtgg agttgccgga tcactggtat gtcgatcaaa
    10261 ctacgctgcg tattctcggc attggcttac tgatgattat cgcggtttat ttgtggtttt
    10321 gcgctttcgc gaagcaccgc catatgacca tcaaagggca aaagctggtg ctgccttcat
    10381 ggaaattcgc cctcgctcag atgctgattt ccagcgttaa ctggatggta atgggggcga
    10441 ttatctggct gttacttggt caaagcgtga actatttctt tgtactgggc gtgttactgg
    10501 ttagtagtat tgctggcgtc atcgtgcata ttccggcggg gatcggtgtg ctggaagcgg
    10561 tgtttatcgc gctactggct ggggagcata catccaaagg cacaattatc gccgccctac
    10621 tcgcttatcg cgtcctgtat tactttatcc cgctgctgct ggcgctgatc tgttacctgc
    10681 tactggaaag ccaggcgaag aagctacggg cgaaaaatga agcggcgatg tgaagtttag
    10741 ttaagttctt tagtatctgc atttacgact aatgaaaaaa acgcgtatgc ctttgccaga
    10801 caagcgttat agctatcgcg gcaagtatcc caaagaagcc gataaaatag taatagagtg
    10861 ttaatgcacc tgtaacagcg caacgaaccg gactccacaa ctcttcacta tggcatttcc
    10921 ccgccaattc aaggagctta tttcgtccat gcgagataat gcctgaccat aacacaatca
    10981 ttaagcaact tattacccaa acgggtcgtt cactcattaa tgcggcattc atgatgatca
    11041 ccagggccaa acctgtcaac gtcatcataa tgataaaacg gtgagaacca ggatcaacat
    11101 taaataataa accggccagc atactgatta aaaacatcgc gcccgcagtg ctgaacacga
    11161 cgataatggc aggtacagga gctaaacttg caacgacaat cgccaccaaa gagagtaaaa
    11221 acagatatat tacgcctgcg atgacggcat ttatcttgtt gatatcctta cttaagccat
    11281 acatacaaac taataataac cctatggcga catacattgt tggtgaatca tatttggttg
    11341 ccgtaatttc gagatactta gctccccagg ccagtcctgc ggttgccacc aaacctaaag
    11401 cggtaatgag ccatataccg tttaatatcc tggtctcgtg ctgaaaatct aatttattgc
    11461 tgttttgtga atatgattcc attcttttag ttgtccttag aaaaaatatt aatattcacc
    11521 atcaataaat ttaaaaacac gcatacaaaa tacttttgta tttatggata cggaaaacgc
    11581 ttccgctcgc cataaggcgt attttatacc tggtacttgt taagtgtcgt ttttagtaag
    11641 aaaaaaaatc ccgacacatg gccgggatat tgatggtgaa aaagaattaa cggcggttgc
    11701 caaaaattcg cagcaacatc aggaacaggt tgatgaagtc cagatacaag gttaacgcgc
    11761 caagaatgga atatttgcgc aggttcgacg tgtcgcgggt atcaatctgc tcacccatat
    11821 ttttcaattt ctgagtgtca tacgccgtca atccgacaaa gacaatcacg ccgatgtagg
    11881 taactgccca catcaatgct tcgcttttca gccagaagtt gaccagcgat gccagcacaa
    11941 tgccgattaa cgccataaac agcatattgc caaagccact taaatcgcgc ttcgtggtgt
    12001 aaccgtacag gctcattgcg ccgaacatcc cggcagtaac gacgaaagta ctggcgatag
    12061 aagcagcggt atagacaatg aatatactgg aaagcgtaag acccgtcagc gccgaataaa
    12121 gcataaagag catcgtcgtt atacctgcgc tcagcttttg aatcatcgct gataacacaa
    12181 taaccaatgc taattgcgcg atgatcagac cgattaaaaa gacacggtta gtaaacaaca
    12241 gctccatcac ggccgcggaa ttagccgcat accaggcaac aaatgcggtc agcaacaagc
    12301 caacggtcat ccagccatag acttgcgcca tataagtttg caagccagcc cggggttgta
    12361 cgattgaatc agaacgtggg aatctgtcca tgacgatctc ctgaagatat aaggaatatc
    12421 ttaaggatac tgcaaaatga tgaggctgtg catcgacgca gcgtaaacgc atgtactgag
    12481 cggtgaaatt gccggacgca gcggtgcctt atccggctaa caaaaaacta ccagcgtttt
    12541 gccgcctgct gatcgctctc ccgagcttca acccagcggt cgccttccgg cgtggcttcg
    12601 cgcttccaga acggtgcgcg ggttttgaga taatccataa taaactgccc ggcttcaaac
    12661 gcactgctgc gatgcgcact ggtgacaccg acaaaaacga tttcatcgcc cggccataat
    12721 tccccgatgc ggtgaatcac agtgacgcgc cccagcggcc agcggttacg cgcttcatca
    12781 acaatttctg ccagtgcttt ttcagtcatc cccggatagt gttcgagggt taatgctttg
    12841 acgctgtcgc ccaggttatg gttgcgcacc ttaccagtaa aggtgactac cgcaccgtct
    12901 tcgtcacgct ccgccagcca cgggtactct tctcctacgc tgaacggctg cggaccaaca
    12961 acaattttgg tttctgccat cttaacctcc ggttaccggc gggaagaaag ctacttcgtc
    13021 gccgtcagtc agcggatggt caaaactcac cagcgtctgg ttgacggcag ccagtaattt
    13081 gccatcttcc agcgccagcg cccagcgatc gctctgcgca gccatgtgct ggcgtaacgc
    13141 ttcaacagtt gggaaatccg cagccacttc ggttgcatct gttcccacca actcgcgcac
    13201 ctgggcgaaa aaaagaactt taatcatccg cttccacctt aaagtcaccc gacttgccgc
    13261 cgctcttcgc cagcaaacgt accggaccaa tcaccatatc tttttgcacc gctttgcaca
    13321 tgtcataaat ggtcagcgcc gccacggagg ccgcggttaa tgcttccatt tcgacaccgg
    13381 ttttcccggt caggcggcat aaggtttcta tacgcacccg attgtgctcc ggctcggcct
    13441 gtaaattgac ttcaactttg ctgagcatca gcggatgaca gagcgggatc agatcccagg
    13501 tgcgttttgc cgcctgaata ccggcaatac gcgcagtggc aaatacgtcg cctttgtggt
    13561 ggcgaccatc aataatcatc gccagcgtct cgctgcgcat ggtgacaaag gcttccgccc
    13621 gcgcttcacg cacggtttcc gctttggcgg agacatccac catgtgcgct tcgccagcgg
    13681 cgttgatatg ggtcagttgc gacatactta tttcttcaaa tgtggatgga aattacacgg
    13741 acgcgtacgg gcatccagct gcggcgcgat gatattttcc catgcggtac ggcacgcttt
    13801 ggtcgaaccc ggcatggcga aaatcagcgt tttgttggcg acgcccgcta ccgcacgaga
    13861 ttgcaacgtg gaagtgccaa tctcttcaaa cgacaacatc cggaacactt caccaaaacc
    13921 ttcaatttca cggtcgaaca acggcagcaa tgcttcggga gcctgatcac cttccgtcag
    13981 gccagtacca cccgtaatca ataccacttg tacatcgtcg ctggcgatcc acgcagatac
    14041 ctgagcgcga atagcgtagc ggttttcttt cacaatggct ttatcgacaa cgtgatggcc
    14101 cgcttcttgc gccgaatcgc gcagatagtg accggaggta tcgtcttctt caccgcgacg
    14161 attagaaacc gtaagaatag caatacgggt cgggataaat tcagtgctta cctgactcat
    14221 ctgatctctc cttttgacgt tttagccgcc aatgtacgat aagttttgcg taataccggt
    14281 gttgttttga tgcaggaaat gggtctgttt cttctcccgc agcgccgctg aaatacgcgc
    14341 ttccagcgcc tgttgctggg tatcgtcttc cagcagatcg cgcaggttaa cgccgccttc
    14401 accaaacagg cagagatgga gtttaccaat ggaggaaacg cgcaggcggt tgcaagtggc
    14461 gcagaagtct ttttcatacg gcatgataag gccaatctct ccggcgtaat ctggatggca
    14521 aaagacttgc gcgggaccgt cgctgcgttg acgtaattgg tggatccagc cgcgacgcag
    14581 tagctcgtca cgcagaacct gaccagagat gtgatgctta cggaagagct cgctgccctc
    14641 gcccgtttcc atcagttcga tgaaacgcag ctggataggg cgatgctgga tccagttcag
    14701 aaaggtgtcg agctggtgat gattaacatc acgcatcagc acggtattga ctttgacctt
    14761 ctcaaaaccg gcctcaaatg cagcatcaat ccccgccatg acctggttga atttatcctg
    14821 cccggtaata gcgtgaaact ggcgggcgtc cagactgtcg acactgacgt taatgccagt
    14881 aagtcccgca tcgcgccagt tcgccacatc gcgttccaga cggtaaccat tggtggtgac
    14941 cgcaatctgg cggatagcgt cgttttcccg cacagcggcg atgatatcgg taaagtcgcg
    15001 gcgtaaagac ggctcaccgc ctgtcaggcg cactttttcg gtgcccagac tggcgaaggc
    15061 gcgcgtaacc cggcgaattt catcgacggt aagaaagcct ttattggtga cgccgctcgg
    15121 tttgtagcca tccggcaggc agtaggtgca acgaaagtta cacacatcgg taatcgacag
    15181 gcgcaagtag taaaacttac gcgcaaatgc atcagtcagt tgtgaagcca tgtacacctt
    15241 tccagatacg ggaggcgaag tcatttcttc cttcgccctg gtggcaatct ttacgagcat
    15301 tgtcacggcc aaagcaccgt atcagttgac ccaggtgcag aggctagagt gtttagtggt
    15361 tatgccgata ctagcgtgta aatgagattt ttaccatcca cactttcgct atgtaatcat
    15421 gtatatagcg tcatgatcgt gctatttcgc tacagaataa gggaaaatgg cggctttact
    15481 ttgatataca tcatcaggga ggcatagcgt gcatcgtcct ttagaggtag aagaaaatag
    15541 tttgtatcct caatattggc aggttaattg ctgtttcccc gcaatttgcg ctactgtagc
    15601 gcggataatc tgactccagg aaactatatg cgcaatcgta cgctggctga tcttgatcgt
    15661 gtcgttgctc tcggcggagg gcatggactg ggacgcgttc tctcatcact ttcgtctttg
    15721 ggttctcgtt taacgggtat cgtcaccacc accgataatg gtggctcgac ggggcgtatt
    15781 cgccgttcag aaggcggcat tgcctggggc gatatgcgca actgcctcaa ccagctgata
    15841 acggaaccga gcgtcgcctc cgcgatgttt gaataccgtt ttggtggcaa tggcgaactt
    15901 tccggtcata atctcggaaa cttgatgtta aaggcgctgg atcaccttag cgtgcggcct
    15961 ctggaagcca tcaatttaat tcgtaatctg ctgaaagtgg atacgcattt gattccaatg
    16021 tcagagcatc ctgttgatct gatggcgatt gacgatcagg ggcatgaagt ttacggcgag
    16081 gtcaatatcg accagttaac tacgccgatt caagagttat tgttaacgcc taatgtaccc
    16141 gcaacgcgtg aggcggttca cgctatcaat gaagcggatc tcatcattat tgggcctggc
    16201 agtttttata ccagcctgat gccaattctg ctgctgaagg aaatcgccca ggcattacgc
    16261 cgcacgccag cgccgatggt ttatatcggc aatctggggc gtgagttgag tttacctgcg
    16321 gctaatttga agctggaaag caagctggca attatggagc agtatgttgg taaaaaggtc
    16381 attgatgcgg tcatcgtcgg gccaaaagtg gatgtctcgg cggtgaaaga gcggattgtg
    16441 atccaggagg tactggaggc cagcgatatt ccgtatcgtc atgaccgcca gttgttacat
    16501 aacgcgctgg aaaaggcgtt acaggcttta ggttaacgga aatccgaagg aaaattccgg
    16561 cttcctattg aagacaaagt gcgcgttgtt tatgccggat gcggcgtgaa cgccttatcc
    16621 ggcctacaaa ccgcgcaaat tcaatatatt gcggagaaaa tgtaggcctg ataagcgtag
    16681 cacatcaggc tgttttccgt ttgtcatcag tcttcttcgc tatcctgtta cgatgcggca
    16741 ataaacaact cacgcaactg atgcaactgg tcacgaattt gtgccgcttc ttcgaactcc
    16801 agattctgcg cgtgttgcat catcagccct tccagttcat ggattttctg ctgcaacgct
    16861 ttcggcgaca tatccatcgg cacattatcc ggctcaacaa tcgggcgcga ttttcctctg
    16921 cccttcgctt tggttttggc aatgttctgc cccagcgcca ggatatcgac cactttcttg
    16981 ttcaagcctt gcggcgtaat gccgtgttcc tcgttgtact tctgctgttt ctcacggcga
    17041 cgttcggttt cgccaatcgc tttcgccatt gatggggtga tcttatcgcc gtagagaatc
    17101 gctttaccgt taacgttacg tgccgcacga ccaatggtct ggatcaacga acgttcggaa
    17161 cgcaggaagc cttctttgtc agcgtcgagg atcgccacca gcgacacttc cggcatatcc
    17221 agaccttcgc gcagtaagtt gatccctacc agcacgtcga actcacccag acgcaagtcg
    17281 cggataatct ccatacgttc gacggtgtcg atatctgagt gaagataacg cacgcgctcg
    17341 ccatgttctt cgagatattc ggtaagatct tccgccatcc gcttggtcag tgtggtgacc
    17401 agtacgcgtt cgttaattgc cgctcgctga cgaatctccg aaagaagatc atcaacctgt
    17461 gtcgccaccg gccgcacttc gataatcggg tcaagcaatc cggttggacg caccacctga
    17521 tccaccacat cgccgccgga tttttccagc tcgtaattac ccggcgtcgc cgaaacatag
    17581 atggtttgcg gcgctaatgc ttcgaactct tcaaacttaa gcggacggtt atccagcgct
    17641 gatggcaggc ggaagccgta ctccaccagt gtctctttac gcgcccggtc accgcgatac
    17701 atgccgccaa tttgtggaat ggtgacatga gattcatcga ccaccagcag cccatcggca
    17761 gggaggtaat caaacagcgt cggcggtggc tcacccggtc cacgaccgga gaggaagcgc
    17821 gagtagtttt caatccccga acagtagccc agctcgttca tcatctccag atcaaactgg
    17881 gtacgctggg tcagccgctg ctcttccagc agtttgttgt tttccaatag cactttgcgt
    17941 ctggcggcca gctcttcttt gatctcctcc atcgcctgta cgatgcgctc gcgcggtgtg
    18001 acgtagtgcg ttttcgggta gatggtaaaa cgtggaatgg tggaaacaat ctgcccggtt
    18061 agcgggtcaa ataacgacaa tcgttccact tcctcgtcaa acagttccac gcgaagtgca
    18121 atgtcatccg attctgccgg gaagatatca atcacctcgc cacgaacgcg gaaagtacca
    18181 cgctggaatg cttgatcatt acgagcgtat tgcagctccg ccagtcggcg cagaatcgcg
    18241 cgctgatcga taatcatacc gaccgtgaga tggagcatca tcttgagata taaatcagga
    18301 tcgcccagac cataaatcgc ggaaacagac gccactacaa ccacatcacg tcgctccagc
    18361 atcgctttgg tggcggataa acgcatctgc tcaatatgtt cgttaaccga ggcatctttc
    18421 tcaatgaagg tgtcggaact cggtacatag gcttccggct gatagtagtc gtagtaggag
    18481 acgaaatact ccaccgcgtt ttccgggaag aactctttca tttcgccata cagctgggcc
    18541 gccagcgttt tgttgggcgc aagtaccatg gttgggcgct gaaggtcagc aatgacattg
    18601 gcaatggtga aggttttccc ggagccagtc acgccaagta acgtctggtg cgccaggcca
    18661 tcttccagcc cctcttcgag acgtcgaatc gcctctggct gatcgccaga aggtttaaaa
    18721 gcggaattca gtttgaacgg tttactcatg agtcgctacc tgaaggagtt gggcgggcag
    18781 gtatgtaatt ttactcgtcg tacttaatta tgccaacaaa ttatactgga taaaaaaaca
    18841 gttcatcacc ataatatttc tgttacagcg taaactccgc tgtcaatcat gagcaaaatt
    18901 tactctgtgg cgagataaaa ctccggcctt accgggttat ccccaaagca acggcttttt
    18961 taacatttgt caagatgagt gatgacagtt ttatggcaag agatgcctgt tcagtgactg
    19021 ccattgcatt tatctaacca gttaaaaaat aaaagatatt tctttgagcc gtctttaacg
    19081 ccaattcgcg tgcaagccgc gtattctctc gcttgcctcg tgttttctaa ctctaataca
    19141 catggttatc cacaggaata gtggataact gtctccagcc cctatccccc gccgcctggg
    19201 caattcccac attcgccgca tgaggcggaa gtaaaatttt tttcgtgatt attttagaat
    19261 ttaattggtt aaattgcagt caatcgaaga cgcgatctcg ctcgcaattt aaccaaatac
    19321 aggatggcta caacaaggca aggtttatgt actttccggt tgccgcattt tctggatttt
    19381 ctgcaagcca ggggatctct cccagcagcg gcgcgggaat catgcgggtg agcgtggtca
    19441 tatattcagc gtgacgtttt cccggaggcg taacatcgtt cgccacccaa cccgccagag
    19501 tcagtccggc gtgttgtatt gcctgcgcag tcaacatcgc gtgattaata cagccgagtt
    19561 tcacaccaac taccagtatc accggcagtt gttcctgtgt tacccaatct gcaaaagtga
    19621 aagtgtcaga aagcggcgta aaccagccgc cagcaccttc cactaacacc cagtcagcct
    19681 gttgttcaag cgcgcgtaat ccggcgctca ttaccaatga ttctatcggc ctgccctctt
    19741 gcgcgctgat gatgtgcggc gaggtgggtt ctgcgaaggt gtaaggattt accgttgcgt
    19801 aatccagctg caggctgctg ttgtgctgta acgccagcgc gtcactatta cgtaagcctt
    19861 ccggggtctt ttcgctgcca gaggcgaccg gtttataacc tgccgtccga taacctactg
    19921 cctttgcggc ttgtaaaagt gcacaactgg cgacagtttt ccccacttcg gtatccgttc
    19981 cggtgacaaa ataacgttta ctcacgagca atcactccca aaaaaagatg atacgtcagg
    20041 ggatatcgcc cctgtttttg tggccaggcc agttgcaatc gctgcaactg cgaacgcgtt
    20101 aatattcgcg ggtcgcgccc ttcatgaaga tgcgtggctc caatgccttt cagcgaacgc
    20161 atggcactga gcgcatcatc aaaccacagc gtgatgggct gaatatgatg ttgataatgc
    20221 acgccgttca gtgactgttc gattgcacct ggtggtaaaa agcgattagc atgcggacgc
    20281 tcgtccactg cctgccacgc ctgatgcagt tcgggtaacg atccctgcac cagcgtggta
    20341 aacgcgacca cgcctccggg gcgcaccacc cgatacagct cgcggagtgc cgtggataaa
    20401 ttaccgcacc actgcaccgc gagattgctc catgcaagat cgaacgtcgc agtcgctaac
    20461 ggcagggatt cgatatctcc cgccagataa tggtctgcgg catccttctg gcgtgcctga
    20521 acaagcattg gcggcgagag atctaacgcc gtcacctgcg cgtgacgctc tcgccagtgg
    20581 cggctcatcc agcctggccc acaccccgcg tccagtacgt gggtgtattt acgctgtggg
    20641 agcattgcca gtaagacgtc agcactctgg cgctgtagat ctgcgtgttg ctcatagtgc
    20701 gcggctgccc gaccaaatgc cgctgcaatg gcttgtttat taaccgttgc catgcagcat
    20761 ctccagcaaa cggtcaatat cctgcatttc atgggcggcg gttaaggtca ggcgtagccg
    20821 cgcggtgcca gcgggtacgg ttggcgggcg aatcgccgtc acccagcaac cttgctggcg
    20881 cagtttttct gccagctgta acgcacggct gttatcaccg acgatcaatg gctggatggc
    20941 gctacgtgaa tccgcaagcg taaacggcga accctgcact ccggcacgaa aacgcgtaat
    21001 gagtgccgcc agtttttcgc gccgagcgtc cccctcttca ctgcgaatga ccgccagcga
    21061 cgcacgtaat gcctgcgcct gagcgggcgg catactggtg ctgtagataa ggtggcgggc
    21121 gaattgcagc agataatccg ccaccgtact ggagcaaagc accgctgccc cgctgacgcc
    21181 aaatcctttg ccaaaagtca ctaccagcaa ttctggtttt accttttgca gccagcagct
    21241 gccgcgcccc tgctccccga taacgcccgt gccgtgggca tcatcgacca tcaaccagcc
    21301 attgtgctgt tgcgttacct gctggatttc cgccagtggc gcactatcgc cgtccatgct
    21361 gaacacgcct tctgtcacca ccatttgctg ccccggacag ggggaagcaa gcaatcgcgc
    21421 caaatgagtg acatcgttat gagcaaaacg gcgaagctgc gacgggctta aactggcagc
    21481 ttccagcaat gaggcatggc taagccggtc ggcagcaata cggtcctctt tcgccatcat
    21541 cgcggcaata actgcctgat tagcggcaaa accagagata aatagcagtg cccgcgaata
    21601 gcccagccac tcggccagtt cttcttccag cgcctgatgc gcaacgctat aaccgctgac
    21661 gtgaccggaa ccgccgctac cgacgccaaa ttgctccgca ccctgctgcc aggcacggat
    21721 aatttgcgga tgatggctta aacctaaata atcgttactg gaaaagttca gatactggcg
    21781 atcatccgcc accagccagc gtccggctcc ttgcgccact ggataacggc gacgcagggc
    21841 atcggcagca cgccgcgcat cgagcgccgc gttgattttc tcctgccagc tcataatgct
    21901 gccgcgttgt aatattcgtc ggtgtccggg gtcatcagcg cctgttcaag acgttgctgt
    21961 tgttcgttat cccctgccag cacggcagtt tgctgcggat ttagccccag tttgcggaac
    22021 agttgcaggt ctttatcttc ttccggattc ggcgtggtca gcagtttgca accgtagaaa
    22081 atcgagtttg cgcctgccat aaagcacatc gcctgagtct gttcgttcat ctgctcacgt
    22141 ccggcagaaa ggcgcacgta agaggttggc atcatgatcc gcgcgaccgc aatggtgcga
    22201 ataaaatcaa aggcatcgac atcatcgtta tcggcaagcg gcgtgccttt caccttcacc
    22261 agcatgttga ttggtacgct ttccggcggc gtcggcaggt ttgccagttg cagcaataat
    22321 ccggcgcgat cttttaccgt ttcgcctaag cccacaatgc cgccagaaca gactttgatc
    22381 ccggcatcgc gcactttttc cagcgtatcg aggcgttcct gataagtgcg ggtggtgatg
    22441 atattgccgt aaaactccgg cgaggtgtcc aggttgtggt tgtagtaatc cagcccggca
    22501 ttcgcaaggc gctgcgcctg agattcactc aacgtgccca gcgtcataca cgcctccagc
    22561 cccatcgctt ttaccccctg caccatttgt tccaggtacg gcatatcgcg ttcgtgggga
    22621 ttcttccacg ccgcgcccat acagaaacgc gtcgatcccg cagctttcgc tttgcgcgcc
    22681 gactccagca cctgttcaac ttccatcaac cgctcggctt ccagcccggt tttgtagcgc
    22741 gagctttgcg ggcagtattt gcaatcttcc ggacaagctc cggtcttaat cgacagcaaa
    22801 gtgctgacct gcacctgacg aggatcgaaa tgctgtcgat gcacctgctg cgcttcaaac
    22861 agcagatcca gcaacggttt ttcaaataat tctgtgactt gcgacaatgt ccagcgtggg
    22921 cggtgagcca tggggcttct ccaaaacgtg ttttttgttg ttaattcggt gtagactcgt
    22981 aaacctaaat cttttcaatt tggtttacaa gtcgattatg acaacggacg atattgcctt
    23041 tgaccaacgc catatctggc acccatacac atccatgacc tcccctctgc cggtttatcc
    23101 ggtggcgagc gccgaaggtt gcgagctaat tttgtctgac ggcagacgcc tggttgacgg
    23161 tatgtcgtcc tggtgggcgg cgatccacgg ctacaatcac ccgcagctta atgcggcgat
    23221 gaagacgcaa attgatgcca tgtcgcatgt gatgtttggc ggtatcactc atgcgccagc
    23281 cattgagctg tgccgcaaac tggtggcgat gacgccgcaa ccactggagt gcgtttttct
    23341 cgcggactcc ggttccgtag cggtggaagt ggcgatgaaa atggcgttgc agtactggca
    23401 agccaaaggc gaagcgcgcc agcgttttct gaccttccgc aatggttatc atggcgatac
    23461 ctttggcgcg atgtcggtgt gcgatccgga caactcaatg cacagcctgt ggaaaggcta
    23521 tctgccagaa aacttgtttg ctcccgcccc gcaaagccgc atggatggtg aatgggatga
    23581 gcgcgatatg gttggttttg cccgcctgat ggcggcgcat cgtcatgaaa tcgcagcggt
    23641 gatcattgag ccgattgtcc agggcgcagg cgggatgcgc atgtaccatc cggaatggtt
    23701 aaaacgaatc cgcaaaatgt gcgatcgcga aggtatcttg ctgattgccg acgagatcgc
    23761 caccggattt ggtcgtaccg gcaaactgtt tgcctgtgaa tatgcagaaa tcgcgccgga
    23821 cattttgtgc ctcggtaaag ccttaaccgg cggcacaatg accctttccg ccacgctcac
    23881 cacgcgcgag gttgcggaaa ccatcagtaa cggcgaagcc ggttgcttta tgcacgggcc
    23941 aacttttatg ggcaatccgc tggcctgcgc ggcagcaaac gccagcctgg cgattatcga
    24001 atccggcgaa tggcagcagc aggtggcggc tattgaagta cagctgcgcg agcaacttgc
    24061 tcccgcccgt gatgccgaaa tggttgccga tgtgcgcgta ctgggggcaa tcggtgtggt
    24121 cgaaaccact cgtccggtga atatggcggc gctgcaaaaa ttctttgtcg aacagggtgt
    24181 ctggatccgg ccttttggca aactgattta cctgatgccg ccctatatta ttctcccgca
    24241 acagttgcag cgtctgaccg cagcggttaa ccgcgcggta caggatgaaa catttttttg
    24301 ccaataacgg gaagtcagcg tgagggtttc tggctacact ttctgcaaac aagaaaggag
    24361 ggttcatgaa actcatcagt aacgatctgc gcgatggcga taagttgccg catcgtcatg
    24421 tctttaacgg catgggttac gatggcgata atatttcacc gcatctggcg tgggatgaag
    24481 ttcctgcggg aacgaaaagt tttgttgtca cctgctacga cccggatgcg ccaaccggct
    24541 ccggctggtg gcactgggta gttgttaact tacccgctga tacacgcgta ttaccgcaag
    24601 ggtttggctc tggtctggta gcaatgccag acggcgtttt gcagacgcgt accgattttg
    24661 gtaaaaccgg atacgatggc gcagcaccgc cgaaaggcga aactcatcgc tacattttta
    24721 ccgttcacgc gctggatgta gaacgtattg atgtcgatga aggtgccagc ggcgcgatgg
    24781 tcgggtttaa cgttcatttc cactctctgg caagcgcctc gattactgcg atgtttagtt
    24841 aatcactctg ccagatggcg caatgccatc tggtatcact taaaggtatt aaaaacaact
    24901 ttttgtcttt ttaccttccc gtttcgctca agttagtata aaaaagcagg cttcaacgga
    24961 ttcatttttc tatttcatag cccggagcaa cctgtgaaca cattttcagt ttcccgtctg
    25021 gcgctggcat tggcttttgg cgtgacgctg accgcctgta gctcaacacc acccgatcaa
    25081 cgtccttctg atcaaaccgc gcctggtacc tcttctcgcc cgattctgtc ggcaaaagaa
    25141 gcgcagaatt tcgatgctca acactatttt gcatccctga caccaggtgc tgcagcgtgg
    25201 aatccttccc cgattaccct gcctgcgcaa cctgactttg ttgtcggccc ggcgggtact
    25261 caaggtgtaa cgcataccac gattcaggcg gcggtagatg cggcaattat caagcgcact
    25321 aacaagcgcc agtatattgc cgtgatgcct ggtgagtatc agggaacggt gtatgtccct
    25381 gctgctccgg gtggaattac tctgtacggt acaggtgaaa aaccgattga tgtgaagatt
    25441 gggctttccc ttgatggggg catgagccct gccgactggc gtcacgacgt caacccgcgc
    25501 ggcaaatata tgccaggtaa accagcgtgg tatatgtacg atagctgcca gagcaaacgc
    25561 agcgacagta tcggtgttct ctgctctgcg gtcttctggt cacaaaacaa tggcctgcaa
    25621 ctgcaaaatc tgaccatcga aaacacgctg ggcgatagcg tagatgcagg taaccatccg
    25681 gcggtggcac tgcgtactga tggtgaccag gtacagatta acaacgttaa cattctcggt
    25741 cgtcagaaca ccttctttgt caccaacagc ggtgtgcaga accgtctgga aaccaaccgt
    25801 cagccgcgta cgctggtgac caacagctac attgaagggg atgtggatat cgtttctggt
    25861 cgcggcgcag tggtgttcga taacaccgaa ttccgcgtgg tgaactcacg tactcagcaa
    25921 gaagcgtatg tgtttgcacc ggctacgctg tccaacattt actacggttt cctcgccgta
    25981 aacagccgtt tcaatgcttt cggtgatggt gtggcgcaac tgggccgctc gctggatgtt
    26041 gatgccaata ccaacggtca ggtggtgatc cgtgatagcg ccatcaacga aggttttaac
    26101 acggctaaac cgtgggccga tgcggtgatc tctaatcgtc cgtttgcggg taataccggc
    26161 agcgtagatg ataacgacga aatacagcgc aatctgaatg acactaacta caaccgcatg
    26221 tgggaataca ataaccgcgg cgtgggtagt aaagtggttg cagaggcgaa gaagtaagag
    26281 ctattaacta tttgccggat gcggcgtaaa cgccttatcc ggcctacaga ttcgatgcga
    26341 ttcgtaggtc ggataagatg cgcaagcatc gcatccgaca ataagtgccg aatgcgacct
    26401 acattcacat ggcgcttttt acatctgacg gtttttattg aagttaatca aactacccgc
    26461 cttgataatc tcgcgctctt cagcagtcag actttccata tagagcgtaa tttccgttac
    26521 cggcgcatct tcatggatca cataaccttt aaacgtcgta cccggattat ccagcgccgc
    26581 tttaatgcca gggatgtaaa tgtaatcccc cacttcaaag gttggcactt ccgccatctg
    26641 cagcggtaac atcccccagt tgatgacgtt agaacggtag cgtttagtcg cgtactcttc
    26701 ggcaatattc gccagaccgc caatcacacg ctggcagctc gccgcctgtt cacgcgcaga
    26761 accatcgcct ggtttcaccg catataccat gctgccaatt tcagtttgca gcggatcaat
    26821 atgctcctga ccagcaatct gcttaatgcg cgcaaacacc tctgtcagct cgctgacatt
    26881 ccccgccaga cgctgatttt ccagctcagc agtcgcttta cttctgccaa cataaccggg
    26941 atcgcggcgt gacagggtaa actccgccag accaatcgga tttgaacgat aagaagaggt
    27001 ttcaccggaa ggaatcagtt cgtcggtggt ggtcacttcg tcgaggatct tcgagcacac
    27061 tttcaggacg atattgtcag tcagcgcacc caattccggc cagtctttaa tgttcggtcc
    27121 gtaaatcagc ggttgctgag ttgccccttt cacaaagccc tgataaacac ggtttttata
    27181 cggcgttaca tcgaaggcgt actccggcac gttgtcccag caatcaagtt cgctggcaga
    27241 ggttaaatag ccaccgtttg ccgcagtcgc agcgatagaa cgagcgtcca tcaacgccac
    27301 cgctgacatc tgcccattag ctggcttaga gccttcgcgg ttcgggaagt tacgcgtggt
    27361 gtggcgaata cttaatccgt tgttgattgg cgtatcgccc gcgccaaagc atgggccgca
    27421 gaacgcggtt ctgatgattg cgcctgcgcc aatcaaatct gctaccacac cttttttggc
    27481 gagatctata aacaccggct gtgatgacgg gtaaactgcc agcgagaagg tgtcattgcc
    27541 acaggattga ccgcgcagtg cattcgccgc cgcgatgacg ttttcgtagt taccgccaga
    27601 acagcccgcg ataatcccct gctgcacttt caggcgacca ttttccactt tatccagcag
    27661 cgagagtttg gctttaccgt gcgccacgcg ttcggactca atttcaatct cacgcagaat
    27721 gtcggtcagg ttctggttca gtgtgtcgat ttcatacacg ttgctcgggt ggaacggcag
    27781 cgcaatcatt ggtttgatgg cgcttaaatc aacgctgatg cagccatcgt agtacgccat
    27841 cggttgaggg ttaagctggc agtaatcctg gccgcgaccg tgcagcgcca gccagttatg
    27901 cacttcttca tcagtttgcc agacggagct taaacaggtg gtttccgtgg tcatcacgtc
    27961 aacgctgtta cggaaatcgg tagagagcgc gctaacgccc ggtccaacga actccatgac
    28021 tttgtttttg acgtaaccgt ttttgaacac cgcgccaatg atagccagcg ccacatcctg
    28081 cggccccaca tacggcgctg gttttccggt cagatgcacc gcaacaactc ccggatagtc
    28141 gatatcccag gtgtcattaa gcagctgttt taccaactca ccaccgccct caccgactgc
    28201 cattgtccct aatgcaccgt aacgggtgtg gctgtctgac ccgaggatca ttttgccgcc
    28261 gcctgccatc atctcacgca tatattgatg gatgaccgca atatgcggag gcacaaaaat
    28321 accgccataa cgctgagctg ccgataaacc aaaaacatgg tcatcaccgt taatggtgcc
    28381 gccgacggcg cagagtgagt tatggcagtt ggtcagcaca tacggcagcg ggaaacgttc
    28441 cataccggac gctttagcgg tctgtacaat accaacaaag gtaatatcgt gagaggctaa
    28501 tgaatcaaac ttaattttaa gtttatccat atttccggac gtattatgcg aagagagaat
    28561 agaccaggca atagtgcctt ttttggcttc ttctttttta atttcgccgg tgaaatgttc
    28621 ttcagcaatt atttcgttat tactggcgag aaacacgcct ttttcagata acttgatcat
    28681 caaatgctcc agatatctgc gcaccgtgaa gtgcgcagaa agataataaa tggatatact
    28741 ctaaataatt tgagttgcag gaaggcgaca agcgagtgaa tccccaggag cttacataag
    28801 taagtgactg gggtgagcga acgcagacgc agcacatgca acttgaagta tgacgagaat
    28861 atttacagca tgttccagcc gatcagcata ttccaccacc acacgccgag ggtgatatgc
    28921 accaggaagg ttaatatcgt cagtaccgca ccgaccaacc accaggattt aatatcgtta
    28981 taacccacgc caaagatgac cggacctgcc gcgccgccat agtgagtaac catgccgcca
    29041 taggagttgg agaacaacag tgccagcgcg gttaacatta acggtgcgcc ggagacgttc
    29101 gccagcatgg caaataccgg taacatagca acgatatagg cactaccgga agcgaagaaa
    29161 taacgcacga taatgctgag gaaaataata acgaagaaag caacgttacc gtgaccatca
    29221 aatgccaggt tatttttaaa gacttcagct aaccattcga agaatttaac tttcgataat
    29281 aaggagctta atccgataat accgccgtac cagattaagg tattccagcc gcctttattt
    29341 ttaaccacgt cttcccaggt aacgataccc agcagcagca tggtcgccat aacaacgatt
    29401 gccacagtgg attcatcaac ccccagagac ttactgaaaa tccagcccag tagcgccagc
    29461 acaaagacac cgagcagcat tttttcgcgg attttcatcg gtcccagttc ggcaaggccc
    29521 gctttagcga tggttttgtt atccaccttt ttaatttctg gtggatacat ggtgtaaatt
    29581 accagcgggg tgaccagcag cataatgatg cccggcaatc cggcggccag cgcccatcca
    29641 ccccagctaa tttgcaggtg cagaatgtcg ttgatcattt tcagcgccag aatgttcccc
    29701 gccattgcgg taaagaacat atagctggtg gttttggtga ccatgtaaat ggacatcatc
    29761 aggtaatgtc cgacacgacg cggacttttt tccggttcgg accccaaagc caccgccacg
    29821 ctgttgatga tcggtaacac aatgccgccc gcacgcgcgg tgttagacgg tgttgccgga
    29881 gccagtacca gatcgaggaa taccgtaacg taacccagac ccagcgtggt gttaccgatt
    29941 ttaccaatca gcagataggc aatacgttta cctaaaccgg tggtcacaaa tgcggcgctt
    30001 aaggtaaacg ccgagaacac cagccaggta gtacctgaag agtaaccgct taatacggcg
    30061 gtggtcttaa acgcaccgtc ggataagtta ccgaccacca ccattgaggc agcaacggca
    30121 attaacagta cgacaggttc cgggaaaggc ttgataacca gacccacaat ggccgccagg
    30181 taaataccaa aaagcaccca cgccagttcg cttaatcctg ccggagctgg cataaaacca
    30241 ataatacatg ggatcgctaa tatcagaatt agcttccata acgatttctt attcatttaa
    30301 aatcctgttc tttctgtatg actcatacag aagtccttta ttgattgtcc ttatcctgac
    30361 aacgaatttt ttcagggaag ataaacttca ccggaaaata tttttctggt cgtccgaata
    30421 acagatgcgc gtaacgtcgt ggcatcctga ccttcattac ttaaatgaac gtcgagcgca
    30481 ccactggggt gttcaatatt gatattgccg tatcctacag aagggacgat ttgtcgggtg
    30541 acggtgcctt ccaatgcaca actactggaa atagcaatag caccggttat cgccagcgcg
    30601 cgatggcaag aatgcggcat aaaataacgt acattaattg ccccgccttt ctgcgctaga
    30661 gaaataagca caggtttagg gataaccata ttactgacat cacctaagcc cattgcttta
    30721 cccgcttgta gacggataga ttcaatgcgt gctaataatg ctttgtcggc atccagttcc
    30781 gccggtaatt cataacctgt ttttcccaga tattcagccg gaataatgac gactggcatc
    30841 gccatatcga tacaggtcac cgggacatcg tcaaaataat caatctgatt atcagtcggg
    30901 aagacttttc cggttttggt tccggcggca ttcaggaaag tgagcgcaac cggtgcggca
    30961 gtacccggta cgccgtcaat tctggcgcta ccctcgtact cgacaacacc atttggcgtt
    31021 tgcacatcag cttcgatgaa cgtaccagta ttgacgttgc ggatacgtac gcgggtaact
    31081 ggtgaagtcg ctgcaatcaa accattttca atggcgaatg ccccaacacc agacagcata
    31141 ttgccacaat taggcgtggt atcgacacgt tgctcatgga caataacttg agcaaacaga
    31201 taatcgacat cagcacgcgg atcgctggaa cggctaataa tggcgacttt actggtcagc
    31261 ggattaccgc cgccaatacc gtcaatttcc agatcgttac cggaacccat aattgccatc
    31321 aatattttat cgcgctgcgt ttgatcttcg ggtaaatgtt ccgctaacaa gaacgcgccc
    31381 ctcgaggttc cacctcgcat catcacgcag ggtatttttt tcataataat gccctcaatt
    31441 taaaaac
//
LOCUS       DAZGSP010000060        29763 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZGSP010000060 DAZGSP010000000
VERSION     DAZGSP010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.060"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            197..1696
                     /gene="pitB"
                     /locus_tag="ACHK74_002931"
     CDS             197..1696
                     /gene="pitB"
                     /locus_tag="ACHK74_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFP0221709.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPHLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1745..2437)
                     /gene="yghT"
                     /locus_tag="ACHK74_002932"
     CDS             complement(1745..2437)
                     /gene="yghT"
                     /locus_tag="ACHK74_002932"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP0221710.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLDVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2611..3324
                     /gene="yghS"
                     /locus_tag="ACHK74_002933"
     CDS             2611..3324
                     /gene="yghS"
                     /locus_tag="ACHK74_002933"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP0221711.1"
                     /translation="MNQRNMSIINSTPVHVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILEVDGR
                     QPADEILQASLRAIHAALS"
     gene            3356..4114
                     /locus_tag="ACHK74_002934"
     CDS             3356..4114
                     /locus_tag="ACHK74_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221712.1"
                     /translation="MDALQTQTVISTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4160..5509
                     /locus_tag="ACHK74_002935"
     CDS             4160..5509
                     /locus_tag="ACHK74_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFP0221713.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5509..6333
                     /locus_tag="ACHK74_002936"
     CDS             5509..6333
                     /locus_tag="ACHK74_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221714.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            6345..6905
                     /locus_tag="ACHK74_002937"
     CDS             6345..6905
                     /locus_tag="ACHK74_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFP0221715.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6936..8006
                     /gene="lptF"
                     /locus_tag="ACHK74_002938"
     CDS             6936..8006
                     /gene="lptF"
                     /locus_tag="ACHK74_002938"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFP0221716.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSSANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLKPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRIKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            8003..9082
                     /gene="lptG"
                     /locus_tag="ACHK74_002939"
     CDS             8003..9082
                     /gene="lptG"
                     /locus_tag="ACHK74_002939"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFP0221717.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     EILDNLAWQSTFTSMNLEELSMPGNSFSVRQLNHYINYLQETGQPSSEYRLALWEKLG
                     HPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VAALGLPMAFVLAALILVYLFDRQH"
     gene            complement(9117..10289)
                     /locus_tag="ACHK74_002940"
     CDS             complement(9117..10289)
                     /locus_tag="ACHK74_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP0221718.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFK
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHSSPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10289..10537)
                     /locus_tag="ACHK74_002941"
     CDS             complement(10289..10537)
                     /locus_tag="ACHK74_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP0221719.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10569..11483)
                     /locus_tag="ACHK74_002942"
     CDS             complement(10569..11483)
                     /locus_tag="ACHK74_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP0221720.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEQALRNGLF"
     gene            complement(11480..13201)
                     /locus_tag="ACHK74_002943"
     CDS             complement(11480..13201)
                     /locus_tag="ACHK74_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFP0221721.1"
                     /translation="MSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLE
                     DQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLA
                     IPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKAL
                     PEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRC
                     VSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPF
                     GYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPCY
                     GLAENALAVSFSDEGSGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHG
                     IEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGY
                     LYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTVQEKIILQIQCRISD
                     EERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASL
                     HVQESLA"
     gene            13572..14714
                     /gene="yghO"
                     /locus_tag="ACHK74_002944"
     CDS             13572..14714
                     /gene="yghO"
                     /locus_tag="ACHK74_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFP0221722.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(14721..15485)
                     /gene="glcC"
                     /locus_tag="ACHK74_002945"
     CDS             complement(14721..15485)
                     /gene="glcC"
                     /locus_tag="ACHK74_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFP0221723.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            15741..17240
                     /gene="glcD"
                     /locus_tag="ACHK74_002946"
     CDS             15741..17240
                     /gene="glcD"
                     /locus_tag="ACHK74_002946"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFP0221724.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            17240..18292
                     /gene="glcE"
                     /locus_tag="ACHK74_002947"
     CDS             17240..18292
                     /gene="glcE"
                     /locus_tag="ACHK74_002947"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFP0221725.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            18303..19526
                     /gene="glcF"
                     /locus_tag="ACHK74_002948"
     CDS             18303..19526
                     /gene="glcF"
                     /locus_tag="ACHK74_002948"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFP0221726.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            19531..19935
                     /gene="glcG"
                     /locus_tag="ACHK74_002949"
     CDS             19531..19935
                     /gene="glcG"
                     /locus_tag="ACHK74_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFP0221727.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            19957..22128
                     /gene="glcB"
                     /locus_tag="ACHK74_002950"
     CDS             19957..22128
                     /gene="glcB"
                     /locus_tag="ACHK74_002950"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFP0221728.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSSYDP
                     QRGAQVIAWVRRFLDESLPLEKGSYQDVVAFKVVDKQLRIQLKNVKETTLRTPAQFVG
                     YRGDTAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRVETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHAMHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            22484..24166
                     /gene="glcA"
                     /locus_tag="ACHK74_002951"
     CDS             22484..24166
                     /gene="glcA"
                     /locus_tag="ACHK74_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFP0221729.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
     gene            24580..26076
                     /locus_tag="ACHK74_002952"
                     /pseudo
     CDS             24580..26076
                     /locus_tag="ACHK74_002952"
                     /inference="COORDINATES: protein motif:HMM:NF023283.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3375 domain-containing protein"
     gene            26082..26831
                     /locus_tag="ACHK74_002953"
     CDS             26082..26831
                     /locus_tag="ACHK74_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HFP0221730.1"
                     /translation="MTGFFDRLINRSVTANAGCEPEPSDEEVTDESVEESLASSETRT
                     LKKIREATQELLKYGLLEEASKPNLYRIVLSHPEEVTRILEPLDLDIGIDEIRGLLYV
                     KVRLDETPAQDEWAHPLVRRQRLNLEQSLLVAILRQHFVAWEQESGTGASQAQIAIDD
                     LLPQLQIYLGDPGSESKERTRLLTLLDQLKGHGLVTSPDAHERIVIRPIIAHLADPIN
                     LQALLAWLREQIAQQTSPNDAREKDSSEEDV"
     gene            26828..28972
                     /locus_tag="ACHK74_002954"
     CDS             26828..28972
                     /locus_tag="ACHK74_002954"
                     /inference="COORDINATES: protein motif:HMM:NF024944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP0221731.1"
                     /translation="MNQVSGLAGKESFILTRIELFNWGGFHGLHQAAIHQDGTAVIGP
                     TGSGKTTLVDALMTLLCANSRYNLASTGGHESDRDLISYVRGVSGPGDGGEGQSHIAR
                     PGKTVTGIAATLEREGKQVRLGALLWFDSTSSSVTDMKRLWLFSDNPGQTLEHWLNVY
                     HEGGTRLLRQMEKEAIGLWTYPNKKQYLARLRDFFEVGENAFTLLNRAAGLKQLNSID
                     EIFRELVLDDHSAFDRAAEVANSFDGLTEIHQELETARKQQQSLQPVALSWEKYQKQE
                     RQLADWQTLESLLPLWFAQQANHLWREKINLLNARLAEAQTSEEQLQSQLDLQKKVVS
                     DCMQRYLQVGGANIDELNERIKDWQKTLGSREALARQYQQLTRNLGLPSDLIQPQLEA
                     NQHEAAALCEQIAVDIKLKQEEAYQKGALSHHITEELRERENERAEIARRPDSNLPAH
                     YQAFRSELAKALNVDESELPFVAELIQVKPEEAQWRGAIERAVGSNRLRILVAPESAQ
                     EALRWVNQRNNRLHVRLLEVKLPHSPARFFDDGFTRKLLWKDHPWREAVKALLAESDR
                     HCVDSPEQLRDTPHAMTVQGLMSGKQRFYDKHDQKRLDKDWLTGFDNRDRLNFLAKEI
                     TTLQEQVKTANAAFEFAKGEVGLLQNQAASFQKIEQIDFDSIDVPGAKSQLDALRERL
                     ENLTRPDSDASVAKAKLDEAQTVESELDNVTR"
     gene            complement(29265..>29763)
                     /locus_tag="ACHK74_002955"
     CDS             complement(29265..>29763)
                     /locus_tag="ACHK74_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0221732.1"
                     /translation="DQEWILASDLLGVGGLPSSLAGLHKRAKNEGWEKRSVTTPGIRG
                     RAFAYRLSDLPAHVVSIITGSAEIKHQPPQKNGGNSELLELIDTLSQEEQELVIYTLR
                     RKGVEKLLEFCNQENQELIALTGIRRLAALSLNNLSDQKVREIFEGDETKDHHFNLTH
                     KEAKA"
BASE COUNT         7208 a         7265 c         7946 g         7344 t
ORIGIN      
        1 ttcatatgtg aatatattaa ccagtggaat acctgtgttt tgattatttc taaagatttt
       61 gaatgaatgc ttattgtctg atacacaaga aataacactc tttttatcgt taaaagatga
      121 tatttcactt tgcccacgcc gtgaaagtca ctgataatgc gtccgttcgt aaattcaaaa
      181 tggcgtaatc taatatatgc taaatttatt tgttggcctt gatatatata cggggctttt
      241 gttattactt gctctggcat ttgtgttgtt ctacgaagca atcaatggtt ttcacgacac
      301 ggcgaacgcg gtggcaaccg ttatttatac tcgtgccatg cagccgcatc ttgcggtagt
      361 aatggcggcg ttttttaact tttttggtgt gttgcttggc gggcttagcg tcgcctatgc
      421 catcgtccat atgttgccaa ccgatttgtt gctgaatatg gggtcaactc acggtctggc
      481 gatggtcttt tccatgctgc tggcggcgat tatctggaac ctgggaacgt ggttctttgg
      541 tttaccggcc tccagttcgc acaccttgat tggtgcgatt attggcatcg gtttaaccaa
      601 tgcgctgtta accggctcat cggtgatgga tgcgttaaac ctgcgtgaag tgaccaagat
      661 tttctcctca ctgattgttt cccctatcgt cggcctggtc attgcggggg ggctgatatt
      721 tctgctgcga cgctactgga gcgggacgaa aaagcgtgac cgtattcacc gcattccgga
      781 agatcgcaaa aagaaaaaag gcaaacgtaa accgccattc tggacgcgta ttgcgctgat
      841 tgtttccgct gcgggcgtgg cgttttcgca cggcgcgaac gacggacaaa aagggatcgg
      901 cctggtgatg ctggtactgg tggggattgc ccctgctggc ttcgtcgtca atatgaacgc
      961 gtccggctat gaaattaccc gtacccgcga tgccgtcacc aacttcgaac actacctgca
     1021 acagcatcct gaactgccgc agaagttgat tgcgatggaa cctccattgc ctgcagcatc
     1081 gactgatggc acgcaagtaa cagagtttca ctgtcatccg gcaaatacct ttgatgcgat
     1141 tgcgcgcgtt aaaacgatgc tgccaggcaa tatggaaagt tacgagccgt taagcgtgag
     1201 tcagcgcagc cagctgcgcc gcattatgct gtgcatctct gatacctccg cgaagctggc
     1261 gaaactgcca ggcgtcagta aagaagacca gaacctgctg aaaaaactgc gcagcgatat
     1321 gttaagcacc attgagtacg ctccggtgtg gatcatcatg gcggtagcac tggcgctcgg
     1381 cattggcacc atgattggct ggcgtcgtgt agcgatgacc atcggtgaga agattggtaa
     1441 gcgcggcatg acgtatgcgc aaggcatggc ggcacaaatg acggcggcag tgtctatcgg
     1501 tcttgccagt tatattggga tgcccgtctc cacaacacac gtcctctcgt ctgcagttgc
     1561 agggacgatg gtggtggacg gcggtgggtt acagcgtaaa acggtaacca gcatcctgat
     1621 ggcgtgggta tttactttac cggcggcaat ttttctttct ggtgggctgt actggatagc
     1681 attgcagttg atttaacctt cctgaaaatg cccggtcctg gcgatcgggc atttccattt
     1741 ttgactagtg ataaccacgc gcggtcataa aatccgtaat cgctttttct gcatcaacca
     1801 acacctgttc cagtggctga ttggcatcga tatcaaccag ttgtgcacca ccaaaggtta
     1861 actgtggcgt tatggctatc tttcttgcca gcgattccct tttgtggtca ggcttacgcg
     1921 cgcaggccac gtctagatcg acattgagtt tgatgaccag atcaggctta tggctcgcca
     1981 tccagtgaaa cgctttacgt tcctggcttg ccagccatga gacaaaacga ccaccttcaa
     2041 cgttaggtgg gaacaccgta ccatcgtaag cgccaggaat ttggtcctga ggataacggt
     2101 cggttagaac aattaacccg cgacgacgac aggcaagcat atgacgaaag cgcagtaagc
     2161 gacgggcgac aaacgctgta attaccagcg ccggaactgg tcctggcaat ttttttgctg
     2221 ttttcacctg atttcgttca attgttttat gtaaggattt tcccatcaac ggtaattttg
     2281 tcactgcacg accgacattt ccggcctgtt ttcctaaatg aactctttcg gcagcaccat
     2341 atttttcgac aacggtaata agatgttcac acaccgttga cttgcctgaa ccatcgctac
     2401 caataacggc aattaatgga ggtgttattg actgcatgaa tatatccttt aatttaaata
     2461 tccattaaaa atatttattt ggttaatatg tttttatgaa agcgtaattc aggtcaatgt
     2521 cacaattaac cattgtcaca ataaggttga acggatattc tgagcacgga ccacgtaata
     2581 ttcaatacat tatttactgc cgggtttttc gtgaatcaac gtaatatgtc aataattaat
     2641 tccacgccag tgcatgttat tgccattgta ggatgtgatg gttcaggtaa atcgaccctc
     2701 acggcaagcc tggtaaatga actggcagca agaatgccaa cagaacacat ttatctcggg
     2761 caatcgtccg ggcgaattgg cgaatggatt tcacagctcc ctgttattgg cgcacctttt
     2821 gggcgttatc tgcgaagtaa agcggcacat gtgcacgaaa agccctcaac accgcctggc
     2881 aatattactg cactggttat ctatctgctt tcctgctggc gggcgtacaa gtttcgcaaa
     2941 atgttgtgta aaagccagca aggctttctg ctcatcaccg accgctaccc gcaggttgaa
     3001 gtgccggggt ttcgctttga tggcccgcaa ttggcaaaaa ccacgggcgg taacggttgg
     3061 ataaaaatgt taaggcagcg cgagctgaag ctgtaccaat ggatggcatc ttatttgccc
     3121 gtattgttga ttcgtcttgg cattgatgaa caaacggcgt ttgcgcgtaa acctgaccac
     3181 caactggcag cgttacagga aaaaatcgcc gttacgccgc aactgacatt caatggtgca
     3241 aagatccttg aagtggatgg tcggcaacca gccgatgaaa ttctgcaagc gtcactacgc
     3301 gcaattcacg ccgcgctttc ctgatcacca aaatggatag gggatgattt aacgaatgga
     3361 tgcactacaa acacaaactg ttattagtac aaccgcgccg cagcccagct acattccggg
     3421 gctaattgcg gtggtcgggt gtgatggcac cggtaaatcc acactgacca ccgacctggt
     3481 gaaatcgctg caacaacact ggcaaaccga gcggcgctat ctggggctgc tctccggcga
     3541 agacggcgac aaaatcaaac gattgccgtt ggttggcgtc tggctggaac ggcgactggc
     3601 ggccaaatcc tcgaaaaccc aaagcatgaa aaccaaatct ccggcgctat gggcggcggt
     3661 gattatgtac tgcttctcgc tgcgaagaat ggcgaatcta cgcaaggttc agcgactggc
     3721 gcaaagtggc gttctggtgg tcagcgatcg cttcccgcag gctgaaattt cgggctttta
     3781 ttatgatgga ccggggattg gcgtcgaacg tgcgaccggg aaaatcagca tgtttctggc
     3841 gcagcgcgaa cggcgtttat accaacaaat ggcgcaatat cgcccggaat taattattcg
     3901 cctgggcatt gatattgaga ctgccatctc ccgcaagcct gaccatgact atgccgagct
     3961 gcaggacaaa atcggtgtca tgtcgaagat tggctataac ggcacaaaaa ttcttgagat
     4021 tgattccaga gcgccctaca gcgaagtgct ggaacaggca cagaaggcgg tttctctggt
     4081 tgccatcgtt tctgaccgcc gaagtttaac ttaaggctgg cgggatactt tttactatca
     4141 acgataaggt caggctgaat tggcgggttt taacatcaaa cattggtttg cagatggcgc
     4201 gtttcgcacc attattcgca atagcgcctg gttaggctcc agtaatgtcg tgagcgcctt
     4261 gctggggctg ttggcgctct cgtgtgccgg taaagggatg acgcccgcca tgtttggcgt
     4321 actggtgatt gtgcaatcgt acgccaagtc gatcagcgat tttattaagt ttcagacatg
     4381 gcaactggtg gttcagtacg gaacaccagc attaaccaac aataatccgc agcaattccg
     4441 caatgtcgtc tcattttcct tctcgctgga tatcgtcagc ggcgcggtgg cgattgtcgg
     4501 tggcattgcc ttactgccat tcctttccca ttcattaggt ctggatgacc aaagcttttg
     4561 gctggcggcg ctgtattgca cgctcattcc ttcaatggct tcctccacgc cgaccggcat
     4621 tctgcgtgcg gtagatcgct tcgatttaat tgctgtacag caggcgacga aaccttttct
     4681 gcgcgcagcg gggagcgtcg tagcctggta ttttgacttt ggttttgcgg gttttgttat
     4741 tgcctggtac gtgtcgaatc tggttggcgg caccatgtac tggtggtttg cagcgcgcga
     4801 attacgccgc agaaatatcc ataatgcctt caaattgaat ctgtttgagt ctgcccgaca
     4861 catcaaaggc gcgtggagtt ttgtctggtc aaccaacatt gcccactcca tctggtcggc
     4921 gcgtaactcg tgcagcacag tgctggtggg gatcgtgtta ggacccgctg ccgccgggtt
     4981 atttaaaatc gccatgacat tcttcgacgc cgccggaacg ccagcgggtt tgctgggtaa
     5041 aagtttttac ccagaggtga tgcgtttaga tccgcgcacc accagaccgt ggttgttagg
     5101 tgtgaagtcc ggtttactgg cgggcggaat cggtattctg gtggcgctcg cggtgtttat
     5161 cgtcggcaag ccgctcattt cgctggtgtt tggcgttaag tatctcgaag cgtatgacct
     5221 gattcaggtg atgctgggcg caattgtgat ttcgatgctg ggcttccctc aggagtcatt
     5281 gctattgatg gcgggaaaac agcgcgcttt tctcgtggcg caaaccatcg cctcaatcgg
     5341 ctacatcgta ttgctgttca tgttctgtca tctgtttggt gtgctgggag cagcgtttgc
     5401 ttacttcggt gggcaatgtc tggacgtggc gctctcgtta attccaacct taaaggcatt
     5461 ttttcagcgc cattccttgc tttataacgc agccggagag aaatcctgat gatgagaaaa
     5521 tatttcccgc ttgaggcgag cgagcgtcta tttgttgcga ttgaagaaga tgatgtcgtt
     5581 gatgcacagg ttagtttgcc gccgactatt gcactaagtt gtaccaccga aatcattcat
     5641 gataattacg cgttatgcct gcaattctgg ctcaatggcg ttgatcgtca ggaattactg
     5701 cgtttagttc gcaaacaagc caaaggcgat gagctgactg ctgatgagcg caaacaattt
     5761 aaatacatgc gagcgcgcta caagcacctg cgttttgcgc aacggctgta tttaaagaag
     5821 catcaggccg gatttttatt cggcaaaacg accgtttttc tggggcgttt tcaggacggc
     5881 tttcgtaacg gtaagaaaaa tattgtgtcg tattacggca acctgttacg gatttattta
     5941 agctctccgg tctggtcgtt ggtgaactat tcattacgcc atagccagtt agaaagcgta
     6001 agcagtttta ttgcctatcg gcaaaagcaa atgcataccc tgaaagagat tattgccaaa
     6061 ccgcgactaa ccggcaggga atttcatgat gtgcgaaaaa tcatcagcca acaggtttct
     6121 tattacgaca ccttaagatc gcttgatcct gaaaataaag aggcgttaca gatttcccgt
     6181 ttcctcgccg ccattaatgg cctgatggga gataaacacg acgacatggt ggcggatgat
     6241 atggaaaacc gccagtcgta tgatgcaccg ttggcgctgg atagcgatat tcgtcagcgc
     6301 ttagagctgc tgatttcgcg ttttccgcta tagccggagc aatgatgtca tctcgtttga
     6361 ttattgcgtt gattatcatg ctcttagccc cgggtgtgca ggcacacaat ttcgtcactg
     6421 gcaaaacggt tacccctgtt tatatccagg aaggcggcga gctgcttctg aatagcgacg
     6481 acgaaattca ctaccaaaag tggaacagca cacaattagc gggaaaagtc cgcattattc
     6541 agtatatcgc cgggcgtaag tcggcgaaga aaaagaactc actgttaatt aaagcggttg
     6601 aagcggcaaa ctttccgcag gatcgttttc agcccaccac cattgtgaac accgatgatg
     6661 ccatctttgg taccggctat tttgtcgttg gcaaaatcga aaaaaataaa cgccgctatc
     6721 cgtgggcgca attcgttatt gacggcaatg gacagggacg cgttgcctgg cgcttgccgg
     6781 agcagagttc aaccattctg gtgctgaata aagccgggca aattcagtgg gcaaaggacg
     6841 gcagcctgac gccggaagag gtagatcatg tcattgcgtt ggcgcagaaa ctgatcaatg
     6901 aataaaaaac tcaaaggatt gagggcgtta gtgagatgaa gctggttgaa cactacatca
     6961 tgcgtggtac gcgccgtctg gtgctgatta tcgtcggttt tctgatcttt attttcgcca
     7021 gctactccgc acagcgttat ctgaccgaag cggcaaatgg gacattagct cttgatgttg
     7081 tgctggatat cgttttctac aaagtactga ttgcactgga gatgttgtta cctgttggtc
     7141 tgtatgtgtc agttggcgtg acgctagggc agatgtatac cgactcggaa attaccgcta
     7201 tctctgcggc ggggggcagt ccgggacgct tgtacaaagc cgttctttat ctggcgatac
     7261 cgctaagtat ttttgtcacc cttctgtcga tgtatggtcg tccgtgggct tatacgcaga
     7321 tttatcaact ggagcagcag tcgcagtcgg agctggatgt tcgccagttg cgggcaaaga
     7381 aatttaacac taacgataac ggacgaatga tcctttcgca gacggttgat caggataata
     7441 atcgcctgac tgacgcgctg atttatactt cttctgccaa tcgaacccgc attttccgcg
     7501 cccgttcggt tgatgtggtt gacccatcac ccgagaaacc gaccgttatg ttgcacaacg
     7561 gcactgccta tcttctcgat catcaggggc gtgacgacaa cgaacagatc taccgtaatc
     7621 tgcaattaca tctgaaaccg ctggatcaaa gccctaacgt caaacgcaaa gcgaaatcgg
     7681 tcacggagct ggcgcgctcc gcctttcctg ccgatcatgc cgaattgcaa tggcgacaaa
     7741 gccgtggcct gacagcattg ttgatggcgc tgctggccat ttcattaagt cggataaaac
     7801 cgcggcaagg gcgattttca acattattgc cactgacgtt gctgtttatt gccatttttt
     7861 atggcggcga cgtctgccgt acgctggtgg ctaacggcgc gattcccctc attcctggtt
     7921 tgtggttagt acccggactc atgctaatgg gcctgctgat gctggtcgca cgcgacttct
     7981 ctttgctgca gaaattttcc cgatgaatgt tttcagtcgc tatttaatcc gtcatctctt
     8041 tctcggtttt gccgccgccg cagggttatt gctgccgctt tttactacct tcaacctgat
     8101 taacgaactg gatgatgtca gcccgggcgg ttatcgctgg actcaggcgg tgctggtggt
     8161 gctgatgacc ttaccgcgta cactggtcga actttcgccg tttatcgcat tattgggagg
     8221 gattgttggc ctggggcagt tatcgaaaaa cagtgagctt accgccattc gcagcactgg
     8281 gttttctatc ttccgtattg cactggtggc gctggttgcc gggatattgt ggactgtttc
     8341 gttaggcgcg atagatgagt gggtagcgtc gccattgcag caacaggcgc tgcaaatcaa
     8401 atcgaccgcc actgcgttgg gggaggacga tgatattacc ggcaatatgc tgtgggccag
     8461 gcgcggtaat gaatttgtga cggtgaaatc gctgaatgaa cagggccagc ctgtgggcgt
     8521 ggagatattt cattatcgcg acgatctctc gctcgaatcc tacatttatg cacgcagtgc
     8581 caccattgaa gacgacaaaa cgtggattct gcatggtgtg aatcataaaa aatggctgaa
     8641 tggcaaagaa acgctggaaa tattagataa tcttgcctgg caatcgacat tcaccagtat
     8701 gaatcttgaa gaactgtcga tgccgggcaa tagcttttcc gttcgtcagc tcaaccatta
     8761 catcaattac ttgcaggaaa ccggacaacc gagcagtgag tatcgcctgg cattgtggga
     8821 aaagctgggg catcccattt taaccctggc gatgattttg ctggcagtgc cttttacctt
     8881 tagcgccccg cgctcgccgg ggatgggcag ccgtcttgct gtaggcgtca tcgttggctt
     8941 acttacctgg ataagctacc aaataatggt caatctggga ttattatttg cgctgagtgc
     9001 gcccgttgct gcactcggtt tacctatggc atttgtgctg gcggcgttga ttctggtgta
     9061 tctatttgat aggcaacatt aaacccgccg tggcgcaaca ccacggcggg ctcacattac
     9121 tgcttcagag tagcaaatgc ctgaatgatg gcatcgattt gctcacgact gtgggcagcg
     9181 tttacgctac agcgcagcag cgtgatgcct gcaggcgcgg caggtggcag gataagattc
     9241 acataaacgc ccagcgaaat aagctcgcgc cagatgcgta aaccttcctc tttcgaaccg
     9301 attattaccg gcactaccgg gctactatgt gaacttaact cataacccag tttttgcaga
     9361 ccgtcataca gatggttggc gttatccatc agcttttgcc gcagctctgg ttgtgtggca
     9421 atagttctta acgacgagcg cacggtcgca atgcaggacg gagaaggaga ggcggtaaaa
     9481 atatacgggc ggctgccgta gcgcagcact tccatcgctt ccgaaccgac agcaaaaccg
     9541 ccaatcgagg ccagactttt actgaaagta ccaacgataa tatccacatc atcttcaacg
     9601 ccgacagctt cagccagtcc gcgccccgtt gcgcctaaca cgccaaacga atgcgcttca
     9661 tcgacaatca aataaccacc gagacgacgt ttgatatcaa caatttccgc cagcggcgca
     9721 acgtcaccga gcatgctgta gatgccctca acgatgataa tcgcctcttt cgcccgttca
     9781 ccgaggcgaa ccatgcgccg ttccagatct ttagcatcgt tatggcggaa acgaataatt
     9841 tccgcgccac cgagagaaca ggcgtcataa atactggcgt ggctgtcggc atccagtaac
     9901 accacggcgt tatggtcggc aagtgcgctg atgacgccta aattagcggt ataaccggtt
     9961 gaaaaaacga tagcggtagg gcgcttgaaa aactcggcaa tttctttctc taacgccagg
    10021 tgcggcgcat aactgccgtt tgccatacga gaaccggtgg tgccggtgcc ttgggccgct
    10081 aacgctgcct gaccttcgct gatggcatca tgattaaagg ttaaaccgag ataattattg
    10141 gtcccggcca gaatcacttt tttattgccg atccggcctt cggtggcgga atacacttca
    10201 tcaatacagg tgccaaacgg tgttaacccg ttgtcactaa attgcagacg ctcgccagca
    10261 aggcgcgcat atttatcgta tagccccatt atttattctc cagccatgga agtagagttt
    10321 ccattaattg cgcaggggtt ttgacatcca gcagaatatt aataggaatg gaaatatcga
    10381 atctgtcttc cagcatcatt aagagatcca tcactttgat cgactccagg ccaagatcgt
    10441 tgacgagatc gctgtcgggc ttaatttcca caccgttttc gaccaaatcc tgtaaacagg
    10501 aaaggatgta atccattact atttcacgat ttaccataga aacgttaaca cacctttaaa
    10561 caagacaatc aaaatagccc gttgcgtaat gcttgttcca ggctaatccc gggaaaccaa
    10621 ttaatatctt cagaaatacg gttattactt gccgaccagt cggcgtgggt taattcacga
    10681 attttcgagc gggtcagcat aggttcttta cctgccaggc gactcaacgc ggtactgata
    10741 tccgcaaggc aggtgagtac cggcagagga ataccaacca ttcgcacgga accgcaacgg
    10801 acgttggcgg caagttgctg tatgcgttgc cagtcatagc cgccagcgac gccatcgcat
    10861 aattcatagg tttgcgtctg tacagtttcg gcgcttaacc actgacccac tgcttgcgca
    10921 aaatcggtga cgtgcaggaa agagagctgt gtatctggtg caccaagtcg tggcagcagg
    10981 ccgcgcagca tccagtcaaa cagcggtttt aactctttat cgccgggacc atacacggct
    11041 gtcgggcgaa aaacgcccag cgtaatttcg tcagccattg cagtcagccg ttgttcggcg
    11101 atgtgtttgg aatttgcgta ccaggagagc tcgggatggc gcgccgccag cgaagagata
    11161 aacagaaaac gttggcaaaa gcagctctct tttgctgctt gcatcaggcg caggctgccg
    11221 tcaacgttac agcgggtgaa aatctcttct ttgtgcccgc gcacttgccc ggcgcaatgg
    11281 accactgcgc tggctccggc aaccagctcg ctaagtgaat gagtatcttc cagcgaaccg
    11341 cgcacccagg tgagattatc attgacgtga gcgcgggcag tacgcgtcaa tgcgcgaacg
    11401 tgaaagccgc gggcgagcag gttatcgata atatatttac cgataaaccc ggtagcgccc
    11461 gtcaccgcga cagtttgatt catgccaggg attcctgcac atgaaggctt gcagcataag
    11521 ccttctgata acgttttttc gcttccgcac gggcaggctt gccggaggat gttcggggaa
    11581 tactgtgggg cggcaacaga tcgatagccg cggttacgcc aaattcgctt tggatccgtg
    11641 ccgccagcgc gtggataagc tgcccgcgac gttcttcgtc gctgatccga cactggatct
    11701 gcaaaatgat tttttcctgg acggtaacaa aagcaatcgc atcgccagaa tgaatttccg
    11761 gttcttgttc cgcaatatat tcaatatcct gcggccagat attacggcca cgaataataa
    11821 tcagatcttt aatacgtccg gtgacataca gataaccgtc cagcagataa ccgaggtcgc
    11881 cggtgtctaa ccagcccgtc gcggcaatct cgtcttgcga aacctggtcg ccaaagtaac
    11941 cgctcatcag gctggggccg gagatgcaaa tatggcctac cacacgttcc gcgaccggca
    12001 tacctgcttc attgcggatt tcaatgccat gttccggcaa cgctttgccg cagttgacga
    12061 aagtcgatac ggcgcgtgtt tcagcacccg gcgcgacggc tttgccctga tattctagga
    12121 tgtcgcgatc cacttcgtta accacaaccc cagagccttc atcagagaag ctgacagcca
    12181 gcgcattttc tgccagtccg tagcacggca tgaacgtttt atcgtcaaag ttaacctgac
    12241 ggaaacattc agcgaattga tggagttgtt ctgcggagat cggctcagca ccaataccag
    12301 cgacgcgcca gcaggaaaga tccagttcag cgagatcttt ttcattcacg cggcgttggc
    12361 acaattcata gccaaacggc ggcgcaacgg aaacggtgcc gcgattttta ctgatcaatt
    12421 taagccattg caaaggacgc atggcaaaat cctgagtgcg caaataatct actgaaagtt
    12481 gcgtggcgac gggggtcagg agaaagccga ccagtcccat atcatggtag aaaggcagcc
    12541 aggagacgca gcggtcgcca gggcgtaatt taatgccatc gtggcttata gcacgtagat
    12601 tagccatcac ttcgcgatgg gtgataatga cgccacgggg aaaacgggtg ctgccggagg
    12661 tgtactggag gtaggcgata tcgttcggaa ctggacgctg gagcgcaaca tcggcttccg
    12721 gtaatgcctt aaaccaggcg tggcttaaaa catgtaattc ggggttgtta tgcgtcgcgg
    12781 cattgaccag tggcaaccac tcatcaccag tgataatggc tgcgggctgg caacttgcca
    12841 gtaaaccctg gagtttggcg ctccaggaat cccgctgacc gacgcccatt ggaatcgcca
    12901 acgggacggc gactaagcca gcatactgac aggcaaaaaa agcctctaca aacccgctac
    12961 tcgtttcggc aatcagcgcc acgcgttcgc cttttttcag gttcagcgat aacaaccgct
    13021 tcgcaccagc ttcggcacgc gtttttaatg tctgatattc cagttgatct tcaagttggc
    13081 aacgtctgtc ataaaaattc attccggcgc tactcagagc ggcgtagtcc aaagcatcaa
    13141 ccagcgttgg aaaatcggca tagcgcatag gtagggaatg cgtaaagatt ttattagaca
    13201 tatacaccac gaaaaaattg gatatcaaaa ataaaatgac aataatgcgg tggattatat
    13261 aggcataaaa gataaatgag atagtatttt catcggtgtg aaaatgaaca ttgatttaaa
    13321 taatgagttt aagttttttg tttttattgt gaatgatatt taatttatga atatattaag
    13381 aagaagcctt gtccggtcgc cttaaatcga caaaaggcat tgaggcgctt ctgtgtagtc
    13441 tctaacctta tcataatcat taataaatac tatttattct ttgtttgtat tcattgggtt
    13501 gattattaag gtgtcgttcg ttgctgttgc aacctgtttt tcaccgttaa ttatttgtta
    13561 tccacagaga tatggaatgc gatctgctga tgattaaaat tgaaaaagta attaataaaa
    13621 atgaccttaa ggcatttatc gcttttccgt catcacttta tcccgacgat ccaaactgga
    13681 tacccccttt attcatagaa cgcaacgagc atttgtctgc gaaaaatccg gggacggacc
    13741 atatcatctg gcaggcgtgg gtggcaaaaa aagcggggca gatagttggg cgtattaccg
    13801 cgcaaataga taccttgcac cgtgagcgtt acggtgaaga taccggtcat ttcggcatga
    13861 ttgacgccat tgatgatccg caggtttttg ccgcgttgtt tggcgcagcg gaagcgtggt
    13921 tgaagtcaca aggtgcaagt aagatcagcg gtcctttcag cctgaatatc aatcaggaaa
    13981 gcggattact gattgaaggt tttgacacac caccctgtgc gatgatgcca cacggcaaac
    14041 cgtggtatgc cgcgcatatt gaacaactgg gttatcacaa aggcattgat ttactggcgt
    14101 ggtggatgca gcgaaccgat ctcactttct ctccggcgct aaaaaaactg atggatcagg
    14161 tgcgcaaaaa ggtgaccatt cgctgcatca atcgtcagcg gtttgccgaa gaaatgcaga
    14221 tcctgcgtga gattttcaac tctggctggc agcacaactg gggatttgtg ccgtttaccg
    14281 aacatgaatt cgcgaccatg ggcgatcaac ttaaatatct ggtgccggac gatatgatct
    14341 atatcgctga gattgattct gcaccctgcg cgtttattgt cggcttgccg aacatcaacg
    14401 aggcgattgc cgatctgaac ggatcgctct ttcccttcgg ctgggcaaaa ttgctgtggc
    14461 gtttgaaagt cagcggtgtg cgaactgcgc gagtaccgct gatgggcgtg cgcgacgagt
    14521 atcagttcag ccgcatcggc ccggtgattg cgctgttatt gattgaagcc ttacgcgatc
    14581 cgtttgcccg ccggaagatc gatgcgctgg agatgtcatg gatcctcgaa accaatacag
    14641 gcatgaataa catgctggaa cgcattgggg cggagccgta caagcgttac cgattgtatg
    14701 aaaagcagat ttaataccag ctaactcagg ttcatctcca gcggcaccgc cgagcgaatc
    14761 aaatggtggc cttccagctc gatatcgtgg agattctttt tcacggtccg cacatgatcg
    14821 cgtgctgcgc gctgggcgac gtgcggcagc cgctgcaaca ccgcgttgta gatcctcgca
    14881 tgctggcgat cgatctgctt tttttgctgt ggtcgatggt agagattatt tacgctggca
    14941 aacactgaat taaacatcag atcggtcaat gattgcagcg taaacaccag cacctgattg
    15001 tgagaagcct gacaaatggc gagatggaaa gcgtgatcca actgcgcatg ttcgatcagc
    15061 gaaatctctt tgttgttctc actggcggcg agcatttttt cataacagcg ggttatcaca
    15121 acaaaatcag cctgcgttcc cagcgttgcc gccagccttg ccgattcgcc ctccagtaat
    15181 gcgcgaacgt cgagcagatc gtacagcgtt cgcggctgcg tactgaacag atggatcagc
    15241 gggctggtgt cctgcacccg attaagccgt gcgacacgag aatcgcgacc ctgcgccgtt
    15301 tcaataatcc cgcgcccgcg cagcacggtc agcccttcac gcagtgcgga gcgtgagaag
    15361 ccgagctttt cacacagtcg acgttccgag ggaagtggct gaccgacctt cagtacgccg
    15421 tcgataatta accgttcgat actctctgca accacttcgc aaatagggcg acgttcatct
    15481 ttcattcccg gacctcgtgc acaggtagga ccaatttttc tgttcttaac tcgcaaaaca
    15541 cgcacatcac gtaagtgatt atgttacatc aatttaacat taagttaacc aagacaaggt
    15601 cacagagctg gaaaaaaaat ggtctgaccg gtagctaaag agatagacga aaacgaaaag
    15661 cccgcttaat aactgttcac agaagcagcg cgcaaaaatc agctgccaca caacacaaca
    15721 caacaaagcg aagcctactc atgagcatct tgtacgaaga gcgtcttgat ggcgctttac
    15781 ccgatgtcga ccgcacatcg gtactgatgg cactgcgtga gcatgtccct ggactggaga
    15841 tcctgcatac cgatgaggag atcattcctt acgagtgtga cgggttgagc gcgtatcgca
    15901 cgcgtccatt actggttgtt ctgcctaagc aaatggaaca ggtgacagcg attctggctg
    15961 tctgccatcg cctgcgtgta ccggtggtga cccgtggtgc aggcaccggg ctttctggtg
    16021 gcgcgctgcc gctggaaaaa ggtgtgttgt tggtgatggc gcgctttaaa gagatcctcg
    16081 acattaaccc cgttggtcgc cgcgcgcgcg tgcagcctgg agtgcgtaac ctggcgatct
    16141 cccaggccgt tgcgccgcat aatctctact acgcaccgga cccttcctca caaatagcct
    16201 gttcaattgg cggcaatgtg gcggaaaatg ccggtggcgt ccactgcctg aaatatggtc
    16261 tgaccgtaca taacctgctg aaaattgaag tgcaaacgct ggacggcgag gcgctgacgc
    16321 tgggatcgga cgcgctggat tcacctggtt ttgacctgct ggcgctgttc accggatcgg
    16381 aaggtatgct cggcgtgacc accgaagtga cggtaaaact gctgccgaag ccgcccgtgg
    16441 cgcgggttct gttagccagc tttgactcgg tagaaaaagc cggacttgca gttggtgaca
    16501 tcatcgccaa tggcattatc cccggcgggc tggagatgat ggataacctg tcgatccgcg
    16561 cggcggaaga ttttattcat gccggttatc ccgtcgacgc cgaagcgatt ttgttatgcg
    16621 agctggacgg cgtggagtct gacgtacagg aagactgcga gcgggttaac gacatcttgt
    16681 tgaaagcggg cgcgactgac gtccgtctgg cacaggacga agcagagcgc gtacgtttct
    16741 gggccggtcg caaaaatgcg ttcccggcgg taggacgtat ctccccggat tactactgca
    16801 tggatggcac catcccgcgt cgcgccctgc ctggcgtact ggaaggcatt gcccgtttat
    16861 cgcagcaata tgatttacgt gttgccaacg tctttcatgc cggagatggc aacatgcacc
    16921 cgttaatcct tttcgatgcc aacgaacccg gtgaatttgc ccgcgcggaa gagctgggtg
    16981 ggaagatcct cgaactctgc gttgaagttg gcggcagcat cagtggggaa catggcatcg
    17041 ggcgagaaaa aatcaatcaa atgtgcgccc agttcaacag cgatgaaatc acgaccttcc
    17101 atgcggtcaa ggcggcgttt gaccccgatg gcttgctgaa ccctgggaaa aacattccca
    17161 cgctacaccg ctgtgctgaa tttggtgcca tgcatgtgca tcacggtcat ttacctttcc
    17221 ctgaactgga gcgtttctga tgctacgcga gtgtgattac agccaggcgc tgctggagca
    17281 ggtgaatcag gcaattagcg ataaaacgcc gctggtgatt cagggcagca atagcaaagc
    17341 ctttttaggt cgccctgtca ccgggcaaac gctggatgtg cgttgtcatc gcggcattgt
    17401 taattacgac ccgaccgagc tggtgataac cgcgcgtgcc ggaacgccgc tggtggcgat
    17461 tgaagcggcg ctggagagcg ccgggcaaat gctcccctgc gagccgccgc attatggtga
    17521 agaagccacc tggggcggga tggtcgcctg cgggctggcg gggccgcgtc gcccgtggag
    17581 cggttcggtc cgcgattttg tcctcggcac gcgcatcatt accggggctg gaaaacatct
    17641 gcgttttggt ggcgaagtga tgaaaaacgt tgccggatac gatctctcac ggttaatggc
    17701 cggaagctac ggttgtcttg gcgtgctcac tgaaatctcg atgaaagtgt taccgcgacc
    17761 gcgcgcctcc ctgagcctgc gtcgggaaat cagcctgcaa gaagccatga gtgaaatcgc
    17821 cgagtggcaa ctccagccat tacccattag tggcttatgt tacttcgaca atgcgttgtg
    17881 gatccgcctt gagggcggcg aaggatcggt aaaagcggcg cgtgaactgc tggggggcga
    17941 agaggttgcg ggtcagttct ggcagcaatt gcgtgaacaa caactgccgt tcttctcgtt
    18001 accaggtacc ttatggcgca tttcattacc cagcgatgcg ccgatgatgg atttacccgg
    18061 cgagcaactg atcgactggg gcggagcgtt acgctggctg aaatcgacag ccgaggacaa
    18121 tcaaatccat cgcatcgccc gcaacgctgg cggtcatgcg acccgcttta gtgccggaga
    18181 tggtggcttt gccccgctat cggctccttt attccgctat caccagcagc ttaaacagca
    18241 gctcgaccct tgcggcgtgt ttaaccccgg tcgcatgtac gcggaacttt gaggagcagg
    18301 ctatgcaaac ccgattaacc gaagagatgc ggcagaacgc gcgcgcgctg gaagccgaca
    18361 gcatcctgcg cgcctgtgtt cactgcggat tttgtaccgc aacctgccca acctatcagc
    18421 ttctgggcga tgaactggac gggccgcgcg ggcgcatcta tctgattaaa caggtgctgg
    18481 aaggcaacga agtcacgctt aaaacacagg agcatctcga tcgctgcctc acttgccgca
    18541 attgtgaaac cacctgtcct tctggtgtgc gctatcacaa tttgctggat atcgggcgtg
    18601 atattgtcga gcagaaagtg aaacgcccac tgccggagcg aatactgcgc gaaggattgc
    18661 gccaggtagt gccgcgtccg gcggtcttcc gtgcgctgac gcaggtaggg ctggtgctgc
    18721 gaccgttttt accggaacag gtcagagcaa aactgcctgc cgaaacggtg aaagctaaac
    18781 cgcgtccgcc gctgcgccat aagcgtcggg ttttaatgct ggaaggctgc gcccagccta
    18841 cgctttcgcc caacaccaac gcggcaactg cgcgagtgct ggatcgtctg gggatcagcg
    18901 tcatgtcagc taacgaagca ggctgttgtg gcgcggtgga ctatcatctt aatgcgcagg
    18961 agaaagggct ggcgcgggcg cgcaataata ttgatgcctg gtggcccgcg attgaagcag
    19021 gtgccgaggc aattttgcaa accgccagcg gctgcggtgc gtttgtcaaa gagtacgggc
    19081 agatgctgaa aaacgatgcg ctgtatgccg ataaagcgcg tcaggtcagt gaactggcgg
    19141 tcgatttagt cgagcttctg cgcgaggaac cgctggaaaa actggcaatt cgcggcgata
    19201 aaaaactggc cttccactgc ccgtgtaccc tacaacatgc gcaaaagctg aacggcgaag
    19261 tggaaaaagt gttgcttcgt cttggattta ccttaacgga cgttcccgac agccatctgt
    19321 gctgcggttc agcgggaaca tatgcgttaa cgcatcccga tctggcacgc cagctgcggg
    19381 ataacaaaat gaatgcgctg gaaagcggca aaccggaaat gatcgtcacc gccaacattg
    19441 gttgccagac gcatctggcg agcgccggtc gtacctctgt gcgtcactgg attgaaattg
    19501 tagaacaagc ccttgaaaag gaataacaaa atgaaaacta aagtcattct tagccagcaa
    19561 atggcgagtg caattattgc cgcaggtcag gaagaggcgc agaaaaataa ctggtcggtt
    19621 tccattgctg ttgccgatga cggcggtcat ctgctggcgt taagtcgcat ggacgattgc
    19681 gcgccgattg cggcttacat ctcccaggag aaagcgcgta ccgccgcgct ggggcgtcgt
    19741 gaaactaagg gctatgaaga gatggtgaac aacggacgta ccgcgttcgt gactgcgccg
    19801 ttactgacgt cgctggaagg tggcgtaccg gttgttgttg atgggcaaat tattggtgcc
    19861 gtgggcgttt ctggtttaac cggagcacag gatgcgcagg tcgcgaaagc ggcagcagcg
    19921 gtgttggcga aataagcgaa aacgaggaga taaacaatga gtcaaaccat aacccagggc
    19981 cgtttacgca ttgacgccaa ttttaaacgt tttgtggatg aagaagtttt gccgggcgtg
    20041 gaactggacg ctgccgcatt ctggcacaat gttgatgaga tcgttcacga tctggcacca
    20101 gaaaatcgtc agttgctggc agagcgcgat cgcattcagg cggcgcttga tgagtggcat
    20161 cgcagtaatc cggggccggt aaaagataaa gcggcctata aatctttcct gcgtgaactg
    20221 ggctacctgg tgccgcaacc ggagcgcgtg acggtggaaa ccacgggcat tgacagcgaa
    20281 atcaccagcc aggcggggcc acagctggtg gttccggcaa tgaacgcccg ctacgcgctg
    20341 aacgcggcga acgctcgctg gggctcactg tacgatgcgt tatacggcag cgacatcatc
    20401 ccgcaggaag gggcgatggt cagcagctac gatccgcagc gcggtgcgca ggttatcgcc
    20461 tgggttcggc gtttcctcga tgaatcacta ccgctggaaa aaggcagcta tcaggatgtg
    20521 gtggcgttta aggtggttga caaacaatta cgcatccagt tgaaaaatgt taaagaaacc
    20581 acgttacgta ccccggcaca gtttgtcggt tatcgtggcg ataccgctgc gccgacctgc
    20641 attttgctga aaaataacgg cctgcatatt gagctgcaaa tcgatgccaa cgggcggatt
    20701 ggcaaagacg atccggcgca catcaacgat gttatcgtcg aagctgccat cagtaccatt
    20761 ctcgactgcg aagattcggt cgcggcggtt gatgcggaag ataaaatcct gctgtaccgc
    20821 aatctgctgg gcctgatgca ggggactctg caagagaaaa tggaaaaaaa cggtcggcaa
    20881 atcgtgcgta aactgaatga cgatcgtcat tacaccgccg ccgatggctc tgaaatttct
    20941 ctgcacggac gctcgttgct gtttatccgc aacgtggggc atttgatgac cattcctgtg
    21001 atttgggaca gcgaaggcaa tgaaatcccg gaaggcattc ttgatggcgt catgacaggc
    21061 gcgattgccc tctatgattt aaaagtgcag aaaaactcgc gcactggcag cgtctatatt
    21121 gtgaaaccga aaatgcacgg tccgcaggaa gtggcgtttg ccaacaaact gtttacccgc
    21181 gttgagacaa tgctcggtat ggcaccgaat accctgaaaa tgggcattat ggatgaagaa
    21241 cgccggacct cgctgaactt gcgtagctgt atcgctcagg cgcgtaaccg cgtggcgttc
    21301 atcaataccg gtttcctcga ccgtaccggc gatgaaatgc attcggtgat ggaagctggc
    21361 ccgatgctgc gtaaaaacca gatgaaatcg acgccttgga tcaaagccta cgaacgtaat
    21421 aacgtgcttt ccggcctgtt ctgtgggctg cgcggtaaag cgcaaatcgg taaaggcatg
    21481 tgggcaatgc cggacctgat ggcagacatg tacagccaga agggcgacca actgcgtgcc
    21541 ggggcaaaca cagcctgggt tccgtcgcca actgccgcta cgctccatgc gatgcactac
    21601 caccaaacca acgtacagag cgtgcaagcc aacattgccc agaccgagtt caatgctgaa
    21661 tttgaaccgc tgctggacga tctgctgact attccggttg ctgaaaacgc taactggtcg
    21721 gcgcaagaga tccaacaaga gctggataac aacgtgcagg ggattctggg gtacgtggtg
    21781 cgctgggtgg agcaggggat tggttgttca aaagtgccgg atattcacaa tgtggcgctg
    21841 atggaagacc gcgcaacgct gcgtatctcc agccagcata tcgccaactg gttacgtcac
    21901 ggtattctga ccaaagaaca ggtgcaggcg tcgctggaga atatggcgaa agtggttgat
    21961 cagcaaaacg ctgacgatcc ggcttatcgt ccgatggcgg ggaatttcgc taactcgtgt
    22021 gcttttaaag ctgccagcga tttaatcttc ctcggcgtga aacagccaaa cggctatacc
    22081 gaaccgttat tacacgcctg gcgtttacgc gaaaaagaaa gtcattaata gagtcaaaaa
    22141 aagccccggc aagttttatt gtcggggcta ttatagtaac tgcggtagta catttatccc
    22201 ggaaataacc ggagcgatta tcagggttta acaatattaa atacccgtag cgcattacta
    22261 gcgggcctat aacaacaata tggacatctt aacgatactc tgcgcctgcg tgtgcagaac
    22321 agaaaccctt acccgatata cttgttatgc cagaggtagc aattgcgtta gcggcgtact
    22381 gttgctgtaa agtataacgc ctatatttta cagttcagtc ctcttttgct tattacaacc
    22441 ttaagtatat caagcatata aagataataa gagactgaac aatatggtta cctggaccca
    22501 aatgtatatg ccgatgggag gactggggct atccgctctg gtcgccctga tcccgataat
    22561 attcttcttc gttgcactcg cggtattacg tctgaaagga catgtcgctg gagcaataac
    22621 ccttatatta tctatcctga ttgcaatatt cgcctttaaa atgccgattg atatggcatt
    22681 tgctgctgcg ggctatggct ttatttatgg attatggcca attgcgtgga ttattgtcgc
    22741 ggcggtgttc ctgtataaat taaccgttgc cagcgggcag ttcgatatta tccgcagctc
    22801 ggttatctcc atcaccgacg atcagcgttt gcaggtgtta ctgattggtt tctcctttgg
    22861 tgcgttgctg gaaggagcgg ctggctttgg tgcgccggtg gcgattaccg gtgcgctgct
    22921 ggtgggcctg ggcttcaaac cgttatacgc ggcggggctg tgtctgattg ccaatactgc
    22981 gccggtggcg tttggtgcgt tgggcgtgcc gattctggtc gccggtcagg taacgggaat
    23041 cgatccgttc cacattggcg caatggcggg acgtcagtta ccgttcctgt cggttcttgt
    23101 gccgttctgg ctggtagcaa tgatggacgg ctggaaaggg gtgaaagaga cgtggccagc
    23161 ggcgctggtt gctgggggaa gcttcgctgt cactcagttc tttacctcta actatattgg
    23221 tccggaactg ccggatatta cttcggcgct ggtgagtatc gtctcactcg ctttattcct
    23281 taaagtctgg cggccgaaaa ataccgaaac ggcaatcagc atggggcaat ccgcaggtgc
    23341 gatggtggta aataagccat cttctggcgg tcccgtgcct tcagaatata gtctggggca
    23401 aatcattcgt gcgtggtcac cgtttttaat cttaacggtg ctggtcacca tctggaccat
    23461 gaagccgttt aaagcgttat ttgctccggg cggcgcgttt tattcactgg tgattaattt
    23521 ccagatccct catttgcatc aacaagtgtt gaaagcggca cccattgtcg cccaaccaac
    23581 gccaatggat gcggtgttta aattcgaccc tctctcggct ggcggcaccg ctatttttat
    23641 tgcggcgatt atctctatct tcatcctcgg cgtgggcatc aagaaaggga ttggcgtctt
    23701 tgccgaaacg ctaattagtc tgaagtggcc gatattgtcg attggcatgg tgctggcgtt
    23761 cgccttcgtc accaactatt ccggcatgtc caccacgctg gcgctggtac tggcaggtac
    23821 aggcgtgatg ttcccgttct tttcgccatt cctcggctgg ctgggggtat tccttaccgg
    23881 ctcggacact tcctctaacg ccctgtttgg ttcactgcaa tcgaccacgg cgcaacaaat
    23941 caacgtctct gacactctgc tggtggcagc aaataccagc ggcggcgtaa ctggcaagat
    24001 gatctccccg caatctatcg ccgtggcctg cgccgcgaca ggcatggtgg gccgagaatc
    24061 tgaactgttc cgctacaccg tgaagcacag tctgattttt gccagcgtta tcggcattat
    24121 cactctgctg caggcgtatg tgtttaccgg gatgttggta agttaataga atagttatgc
    24181 ctgacgttaa tacaaaccca gtctatcaga ctgggtttgt tagtatttcc gatatcagac
    24241 agggaaaatg cctggtcaga aatgaggcgt aattcggttt tctaaaaaaa gtaaaaaaaa
    24301 cgccttgtgg gtggggatta tcttacgaca tgcactttgt tccatacttt taaactcccc
    24361 attgttatga agtaaaggcg ttgtcttttt atccgttcaa actttagctc aaatttgtag
    24421 catgcagatt ccggtatttt tcgtgatacc agaaaaaagt cactggagga aatgttgtga
    24481 taaaaatcac aaagtaagtc aggttactgg agataaagtc tgcaacagag aattatcgta
    24541 gtctgtgtac ctcaaccagt attttaaagg cccagcttca tggaagaaaa cacaaggcag
    24601 cgtacagaaa attatatttc agcaaaaaac cagcatcccg cctggatact ccttgccact
    24661 cgccgtgctc cactggtatt gagttgcctg aaaacgcttt ttgaaaaatc acatgatggc
    24721 attccccttg aggaagctat tcagtctctt tccagtattc tcattgagca tgtgagtcag
    24781 gaacagtatg acattaatca ggacaatcca tttcttcagg ccagtcgtga actgcgggag
    24841 tggattaaac gccgtttaat tgtcgaacgg gatggccgta tttttgctac cgatgcgctt
    24901 gaggtcgcga ttacgtttgt agagtctctg gataatcgtt ttatgacctc tacggcttcc
    24961 cgtttgtcca ccgttcagcg agaaattgaa aacctggaaa cacggcttaa tcctaacccg
    25021 gcaaaccggg ttgcaacctt acgtcgtcgt atttctgaac tggaacgcga actacaggag
    25081 gcagaagccg ggcatattga ggtgctggaa acgcaccagg cggttgagca tattcgtgag
    25141 gtctacaatt tagcttccag cctgcgggct gactttcgtc gtgttgaaga ctcctagcgt
    25201 gaggctgacc gcgctcttcg ccaatccatt attggtgagc aatatcatcg cggcgatata
    25261 gtagaacgcc tgctcaacga tcaggacgcg ctgcttaata cgccggaagg aagagttttc
    25321 gacagctttc agcaacagct ccgtcaatct tctgaactta aagcgatgag tgaacgatta
    25381 cgtgtgatcc ttagccatcc ttctgcttct gatgcgctta accgtctaca gcgtcacgat
    25441 ctgcgctggc tggttaaacg tctggtcgat gaatcgcaaa ctgtcctcca ggctcgcgcc
    25501 cgtagcgagc gtgatgtccg tggtttcatg aaaaccggtc ttgctgctga acatcatcgt
    25561 gttggtcatt tgcttaatga gtttctgaat ctggcgttaa aactcgactg gcagcgccag
    25621 atgattcgta aacaagaggt accgctacct gccgttggtg tggcggtaac aggcatcccc
    25681 gctattgaac gtttacgttt taaagaggtg gatgatgaag cagagcagac gttggatctc
    25741 agtaatcatg cggcggattt aacgcagatt ggcgatgatt tctgggatgc cttcaatggc
    25801 ctcgatcgtg aggtgttgat tcagcagacg ttgcagcttc ttgcaaaaga aaatcgcccc
    25861 gttggcctgg ctgaactggc ggcgctgtta cccccggctc acgatctcga aacattcgcc
    25921 gtctgggttg gtatggcaag agaagcgggt attgaggtta ttgattcaca gcgtgaattt
    25981 gcagagctta gtgatgggga aggtcgccgc tggcgattta atctgcccac caccggtctg
    26041 gaaagtcagg cattaatgga tatcgattgg gagtaacagg tatgacgggc ttttttgaca
    26101 ggctgattaa ccggagcgtt accgcaaatg caggctgtga accagaacca tcggatgagg
    26161 aggttaccga tgagtcggtt gaagaaagtt tggccagtag tgaaacgcgg acgctaaaaa
    26221 aaatacgtga agcgactcag gaattgctca aatacggcct gctggaagag gccagtaagc
    26281 ccaatctgta ccgcatcgta ctgagccatc ctgaagaggt tactcgtatt ctggagcctc
    26341 tcgatctgga tatcggcatt gatgagatcc gtggcctgct gtatgtcaaa gtcaggcttg
    26401 atgaaacgcc tgcgcaggat gaatgggccc accctctggt gaggcggcaa cgactaaacc
    26461 tggaacaatc tttattagtt gccatactgc gccaacattt tgtggcctgg gagcaggaaa
    26521 gcggaaccgg tgccagtcag gcacagatcg ccatcgatga tcttctccct caactccaga
    26581 tttatcttgg cgacccgggc agtgaatcaa aagaacgcac ccgattgctt accctgctgg
    26641 atcaattaaa ggggcatggg ctggtgacgt cgccggatgc acatgagcgg atcgtcatcc
    26701 gtccgatcat tgctcatctt gccgatccga tcaatcttca ggcattactg gcatggctgc
    26761 gagagcaaat tgcccagcaa acctctccaa atgacgcgcg agaaaaggat agcagcgagg
    26821 aggacgtatg aatcaggtat ctggcctggc tggaaaggaa tctttcattc ttacccgtat
    26881 tgagttgttc aactggggcg gttttcatgg attgcatcag gcagcaatcc atcaggatgg
    26941 taccgccgtt attggcccaa ccgggagcgg aaaaacaacg ttggtcgatg ccctgatgac
    27001 tctgctttgt gccaattctc gctacaatct ggcctcaact ggcgggcatg aaagcgacag
    27061 ggatcttatc tcttacgtcc gcggcgtatc cggtcctggc gatggcggtg aagggcaatc
    27121 gcatattgcc agaccaggta aaaccgtgac aggtattgct gctacgctgg agcgcgaagg
    27181 aaaacaggtc cgtctggggg cgttattgtg gtttgattcc accagttcca gcgtgacgga
    27241 tatgaaaagg ctctggctgt tcagcgataa tcccgggcag acgctggagc attggctaaa
    27301 cgtttaccat gagggaggta cacgtttgct ccgacagatg gaaaaagaag ccatcgggct
    27361 ctggacatac ccgaataaaa agcagtatct ggcgcgcctg cgggatttct tcgaggtggg
    27421 ggaaaacgcg tttacgttgt taaaccgggc ggcagggtta aaacaactca acagtattga
    27481 tgagattttt cgcgagctgg tgttagacga tcactccgcc tttgatcgcg cggctgaagt
    27541 ggccaatagt tttgatggtc tgactgagat tcatcaggaa ctggaaaccg ctcgcaaaca
    27601 acagcagtct ttacaacctg tcgccctgag ctgggagaag taccaaaagc aagagcgtca
    27661 actggctgac tggcagacgc tggaatcgct gttacccctc tggtttgcac aacaggcaaa
    27721 ccacctctgg cgggaaaaga ttaaccttct taacgcaagg ctcgctgagg cacaaaccag
    27781 cgaagaacag ctccagtcac agcttgattt gcaaaaaaag gtggttagcg actgtatgca
    27841 gcgttatctc caggtcggcg gtgccaatat tgatgaactg aacgagcgta tcaaggactg
    27901 gcaaaaaacg ctcggcagtc gagaggcatt ggcgcgtcaa tatcaacagc tgacccgtaa
    27961 ccttggattg ccgtctgatc tgatccaacc acagctggag gcgaatcagc atgaagccgc
    28021 ggccctatgt gagcagatcg ccgttgatat aaagttgaaa caggaagaag cgtatcaaaa
    28081 aggtgcgctt agccatcaca ttaccgaaga gcttcgggaa cgggaaaatg aacgcgccga
    28141 gatcgcgcgt cgtccggatt ctaaccttcc cgcacattat caggcgtttc gtagcgagct
    28201 ggcaaaagcg ttgaacgtgg atgagtctga actgcctttc gtcgctgaac tgatacaggt
    28261 taaaccggaa gaggcgcaat ggcgcggtgc gattgagcgt gctgtcggga gtaacaggct
    28321 gcgtattctg gttgcgcctg aatcggcaca ggaagcgctg cgctgggtaa atcagcgcaa
    28381 caaccgtctg catgtgcggc tactggaggt caaattaccg cactctcccg cccggttctt
    28441 cgacgatggc tttacccgca agctgttgtg gaaagatcat ccgtggcgag aggccgtcaa
    28501 agcattactg gccgaaagcg acaggcattg tgttgacagc cctgaacaac tgcgtgacac
    28561 gcctcatgct atgacggtgc agggattaat gtctggtaaa cagcgttttt atgacaagca
    28621 tgatcagaaa cgccttgata aagactggct gacgggattc gataaccgcg atcgcctgaa
    28681 cttccttgct aaagaaatta ccacgcttca ggagcaggtt aaaaccgcaa acgcggcatt
    28741 tgagtttgcg aaaggcgagg tggggctttt gcaaaatcag gctgcttcct tccagaaaat
    28801 agaacaaatt gatttcgata gcatcgatgt tcccggcgcg aagagccaac tggacgcgct
    28861 cagggagcgg ctggaaaatc tgacccgccc ggattctgac gccagtgtag cgaaagcaaa
    28921 acttgatgag gctcaaacgg ttgaatctga actcgataat gtaaccaggt aaatcatttg
    28981 ataccgacaa gggttttagt gaaaggaggt gaaatcacct gtaagatact gaaataaaac
    29041 aggtgatcga tgattctgga ggttgtttag ttttgatact gattaaataa aatccggtat
    29101 aaaattaagg tatcggcaac aaatttgata ctgcacatac tgcttatgga cgtttttaaa
    29161 agtacttaaa atgtctttta tgctaaaaag tttatatcaa tatcgatctg gtcacaaatc
    29221 aagttgaaag tgctcgctaa gtgcatggcc tgggcttcat ggggttacgc tttggcttct
    29281 ttatgggtta aattgaaatg atgatccttg gtttcatctc cctcgaaaat ctctctaacc
    29341 ttttgatctg ataaattatt aaggctcaat gctgccaggc gtcgaatacc tgttaaagcg
    29401 atcaactctt gattctcttg attgcagaat tccagcaatt tttctacccc tttccttctc
    29461 aaggtgtaaa taactaattc ttgctcttct tggctcaaag tgtcgatgag ttcgagcaac
    29521 tcgctattgc caccgttctt ttgagggggc tggtgtttta tttctgcact acctgtaatg
    29581 atggaaacaa catgtgcagg taagtcgctt aaacggtagg caaatgctct ccctctgata
    29641 ccaggtgtag ttaccgaacg tttttcccat ccttcatttt ttgctcgttt atgaagccca
    29701 gccagagatg aaggaagacc tccaaccccc aacaagtcac tggcaaggat ccattcttgg
    29761 tca
//
LOCUS       DAZGSP010000061        29620 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZGSP010000061 DAZGSP010000000
VERSION     DAZGSP010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.061"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(130..777)
                     /gene="gloC"
                     /locus_tag="ACHK74_002956"
     CDS             complement(130..777)
                     /gene="gloC"
                     /locus_tag="ACHK74_002956"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFP0221733.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(804..1352)
                     /locus_tag="ACHK74_002957"
     CDS             complement(804..1352)
                     /locus_tag="ACHK74_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFP0221734.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="ACHK74_002958"
     CDS             complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="ACHK74_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP0221735.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGLTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="ACHK74_002959"
     CDS             complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="ACHK74_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFP0221736.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="ACHK74_002960"
     CDS             complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="ACHK74_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFP0221737.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="ACHK74_002961"
     CDS             complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="ACHK74_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFP0221738.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="ACHK74_002962"
     CDS             complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="ACHK74_002962"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFP0221739.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            11026..11805
                     /gene="elyC"
                     /locus_tag="ACHK74_002963"
     CDS             11026..11805
                     /gene="elyC"
                     /locus_tag="ACHK74_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFP0221740.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(11782..12675)
                     /locus_tag="ACHK74_002964"
     CDS             complement(11782..12675)
                     /locus_tag="ACHK74_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFP0221741.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="ACHK74_002965"
     CDS             complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="ACHK74_002965"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFP0221742.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGADR
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="ACHK74_002966"
     CDS             complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="ACHK74_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFP0221743.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(13806..15038)
                     /gene="ycaQ"
                     /locus_tag="ACHK74_002967"
     CDS             complement(13806..15038)
                     /gene="ycaQ"
                     /locus_tag="ACHK74_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFP0221744.1"
                     /translation="MSLPHLSFADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="ACHK74_002968"
     CDS             complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="ACHK74_002968"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFP0221745.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQMIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="ACHK74_002969"
     CDS             complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="ACHK74_002969"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFP0221746.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(17843..20107)
                     /locus_tag="ACHK74_002970"
     CDS             complement(17843..20107)
                     /locus_tag="ACHK74_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFP0221747.1"
                     /translation="MKITTVGVCIISGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPASGLVMYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTMVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ECWQWLSFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="ACHK74_002971"
     CDS             complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="ACHK74_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFP0221748.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="ACHK74_002972"
     CDS             complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="ACHK74_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFP0221749.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="ACHK74_002973"
     CDS             complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="ACHK74_002973"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFP0221750.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="ACHK74_002974"
     CDS             complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="ACHK74_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFP0221751.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYITSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="ACHK74_002975"
     CDS             complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="ACHK74_002975"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP0221752.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(25685..26773)
                     /gene="serC"
                     /locus_tag="ACHK74_002976"
     CDS             complement(25685..26773)
                     /gene="serC"
                     /locus_tag="ACHK74_002976"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFP0221753.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(26972..27664)
                     /locus_tag="ACHK74_002977"
     CDS             complement(26972..27664)
                     /locus_tag="ACHK74_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFP0221754.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            27794..29554
                     /gene="ycaO"
                     /locus_tag="ACHK74_002978"
     CDS             27794..29554
                     /gene="ycaO"
                     /locus_tag="ACHK74_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFP0221755.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         7184 a         8055 c         7195 g         7186 t
ORIGIN      
        1 tcccctgcaa tattttgaat ttgcacgatt ttgtaggccg gataaggcgc tcgtgccgca
       61 tccggcataa acaaagcgca ctttgccaga aatccaggcc cgcattaaaa gcgggctttt
      121 ttatgtgcct taccagacgg gcatttcgtc ttgcaggaag ggattatgca ggcgttcata
      181 accaagtgtg gataatggtc cgtgacccgg aataaatgta acgtcatccc ccagtggcag
      241 caatttatct ttgattgaag aaatcagttg attatgatcg ccacgcggga agtcactgcg
      301 ccctactccg cctttgaaaa taacatcgcc agaaatcagc agctttgccc gatcatcaaa
      361 aaacacgaca tgacccggcg tatgcccagg gcaatgtaac acctgtaaag tcacattccc
      421 tatgctgatg gtatcgcctt cgttcagcca acgatctggc gtcagcggct ggcactcttc
      481 cagaccaaac atacgacttt gcgcaggcaa gccttgcagc cagaactcat cttctttttc
      541 cgggccaaaa accggcacgc cgtaatgttg cgccagttcc gccgctgcgc caacgtggtc
      601 cagatgacca tgcgtcagca ggatctgcat cagtgtcagg ccgctgtcat caacttcctg
      661 tttgattttt tccgcatcgc cgccaggatc gaccagtgcg gccagacggg tttgttcaca
      721 ccagattaat gaacagttct gggagaatgc ggtgaccgga ataatacgat agttcatact
      781 gcccctgttt cgttaagcaa ttactaccag tgccgtgctg gcccggtatc aatatgcaca
      841 aagttactac gtggataata tcctacacca cctgcgcgca tagataacgc ggctttgcga
      901 atattgctta acgcgatacc ttcaatatgg aaatccatcg cctggccttt agtgtgatag
      961 cttttcttcg ctactccacg gctgcgggcg cgtagttcat tgttggtatc aatagaacga
     1021 taaccggaaa tgagttgcac cggtttgcgc gtgcctaaca acccttgcag gcgatacaac
     1081 tggtcgaata atcctgggtc gatggacttt attttgttcg cgcggtaatc gcggaaaaaa
     1141 tggttaagtt ttgccaattc ttcctgaata tagcctctgc catcgaaaaa ctccgctttg
     1201 attgactctc cggtatgaag attattgagt gtcaaaatgc gcgggcgtgg ggtagagagt
     1261 gttgcaaacg caggggtcgg caggatggcg gcaccgagtg caacgccacc aagcgccagc
     1321 aatttgcggc gattagcgtc gaatttgtcc atgataatca agtctacagg tcaatgttat
     1381 cgtttatatg cacttctggc gcacaagagg caccttaacc gcccgtaaag caggcgtcaa
     1441 gatgactcaa ccccagcagt gacgggggct gcagagaatc gcccccaatc atgctaatta
     1501 ttacgacaac tgatttcccc gaactacttc atttacctga ttaattgttc cgctttcgat
     1561 acgatttgcg agctggatcg cgcaggcaga tcataattgt aaatatctgt acgatactgg
     1621 gtaagaccat ctgcaccaac aaaggccgtc aggtagtaga gattcaccgg aatcgactgc
     1681 cgaatattga cgtaacgtgt atcaccctgc ttcagcgcat cagaaatacg tttgtcattc
     1741 cagcctgcat cctgcaacag catattcgcc agatcggaag ctttattcac tcgtacacag
     1801 cctgagctca atgcgcgtgt atcacgcttg aacagattgt ggttcggcgt gtcatgcaaa
     1861 taaatggcct ctgaactcgg catattgaat ttatagcgcc ccagcgagtt ccgtgggcct
     1921 ggagcctgct ggaagcggaa cggtaaattc gaggccgtga ttgtagacca gtcaacctgc
     1981 catgggtcaa tcgcttctct gctgttccag ccgcgcatca ccgtatagcc atggctttcg
     2041 agatatcccg gatcgttgcg cacttttggc agaatatctt tgcgtgccag agttggaggt
     2101 acgttccacg gcgggtttac cactacgttg ttaagggcac tgctcatcat cggcgttttg
     2161 cgatcggggc gaccgacaat gactcgcgaa tccagcacct gattgccgtt ctgatagtag
     2221 accagcgaat aggccggaat gttaaccatg atcccggtag aaagctctgt tggcagcaag
     2281 cgcaatcgct ggatgttgag agccaacacg ccagcacgct gggcgggcgt tacgtttaac
     2341 cagtcacgtg ttgccgggcc aatagcacca tctgccccca atccttgcca tgcctgaaaa
     2401 cgtttaacgg cttccaccag ttcattatcg taggcggcgc gaacggcagg cgcaggttta
     2461 ctacgagatg tcgtttgctt gtccatcggc ttagtttctg ctgtttcaac agtaacagcg
     2521 gatgggctga ctaccgcgtc agttggcgtg tcatcgccag gtagagtaat tttcggcccc
     2581 ccgtccaaca tgcctgtgcg ttgcaatatt tcgcgcaacg ccggtacgtc gttactccac
     2641 tgccctgggc gcaacgttgc tttgccggtc agttggggcc acggtttggt gtcactgagt
     2701 aaggccagta acgattcatg catcgccgca tattgcggat gctgcggtgc cagtccagca
     2761 acaaacgtag gcaattgacc tttatccagc gccagctgcc attggttaat caccgagagc
     2821 ggcggcgttg caagtgcata aggtttactg ctatatagcc agcgagtgcc tttgaccgga
     2881 atatttgcaa tgaaatggag atagcccatc atcgcatcag agagcactac gtcgcgtgcc
     2941 atcccgttaa caccaggatc ggtcagtaac tctacccatt tattaaactg cggctggaaa
     3001 ccggcaatcg ccacctctgc cagctgttgc tggaaggctt taacagcatc acggttttcc
     3061 cacatgggtt gcatatcgcg tgcggcatac aacagttgaa gctggttaag ataaaccggc
     3121 ttgtaacctg cgggtaattg agattcgatt tgcgtgcggg ctttttctgc cgcaccttca
     3181 ggcaatggct ggatccccgc cattattgcc gtggcttgcg cctgcggcag tgcctcgccc
     3241 tgttcactga cagccaccgg gctgtcgcca gggattactt caggctcatc ggcctgcgca
     3301 ttgaacagtg gagcgaatgt tacggccagg cacaaactga ttgccgacag ccgacgacca
     3361 cacatcatat taagcaacat cccttgcccc ctgtttttat catactctga atagccaggc
     3421 tgttatttta cgacttgcct gtacaacgcc aatactcacg aaagtatgac gagcaagcct
     3481 gttactcatc aggcttatct tcagtatata aacgccgata cgtttttaca taacctaatg
     3541 taaagaagtt taaagaaaag cgcgccgtaa tgtgccactt ttttagtgca gaaacggagt
     3601 tttcggaaaa agaaaaggcg gcattgctgc cgccttaatt ttaactcgcc tgagaaggcg
     3661 cttcgtcact tcctggaagc gtttcaggga gttgcggcgc aaatcctcgc aggccgacga
     3721 cgtgaacgtg ttcggtattc tggaagactt tacgcaccag tttataggtg gtgcctttct
     3781 ccgggctgat attttccggc gctgcgatga tgagctgcat ttgcaaacgt tcacacaatt
     3841 caaacagcgt ggcgatagaa cgagcatcca gtcgcgctgc ttcatcgagg aacagcaggc
     3901 ggcaaggaga gatatcttta ccgcgcaggc ggcgagattc atcttcccag ctttgtacca
     3961 ccatcaccag aatcgacata ccggtaccaa tcgcctcacc ggtcgacaat gcaccagact
     4021 ctgcgcgcaa ccagccatcg gaaccacggt taacctcaac ttccatttcc agatagttgc
     4081 ggtaatccag cagttcttcg ccaatggtct gcggcgtgcg ctgccccata tcaatctgcg
     4141 gattaagacg ctgatacaat ttcgccagcg cttccgagaa ggtcagacgg ttgctgttaa
     4201 acagatcctg atgctgctcg tgctgttcag agagcacatc cagcagcatg gcgtgcgttt
     4261 cacgcacgtt cacgttcaga cgtacgctgt tcacctgacc aaacgatacg ttctgcaacc
     4321 cctggttgag catacggata cggttctgct cgcgctgaat ggttttgcga atgatgttcg
     4381 ccacgctgcg ggaactgatc gccagtttct gttcgcggga ggttaactcc tcggtcagtc
     4441 ggctaagttc gatttccatc tgctcgatgg cttccaccgg atcatcggta cgaataatat
     4501 cctgacgaat acgttcacgc agatgctgat aaaccgccac gaagaactga attttacgtt
     4561 ccggacgttt tggatcttcc gacatgcgca gcacgtcgcg cagatgttcg ttatccgcca
     4621 ccgccagacg cagcgcaccc aatgccttat ccgacataga gcgcaaatca tcagcggaga
     4681 gataagccag ctcgcgacgg tgtaagcgcc gttcaacgcc gttatctttc accatgcgca
     4741 tcactgcaca ccagcccgct ttggcggtca ctacctgctc gcgcatctca aagtaatccc
     4801 gctccagctt gcgcagtttg cgggtcagat tgtccatctc cgcttcgcag aaagtcagtg
     4861 ctttttccag ctggttgcgg cgggaccggt tattactcag ttgcgcatgc agctcgtcac
     4921 ggcgaattcg cgcccgctct tctgccccgc tatcggcgcg cacgccgata tcctgcaatt
     4981 cacgttgcag atcgttgagt agctcttttt tggtgtcgta agaacttttc agcgaagcca
     5041 gcacctggtt gtactgactc aactgcgctg cgtgaccacg caacgcttca cgagcgcgag
     5101 tacgctccgc ttccgcctgt tccagacgtt cacgcagctt ttcgttgaga tcgctattac
     5161 cgctcagcat ttctgccgag tcagaatagc taaagtgcgc acgacgctgc accacttccg
     5221 tcagggcaaa cgcctgctga cgggcatcgc gctgcatctg ctgagagtac gcgtaatctt
     5281 cttttagctg ttcgaactgt tccgggtcgc tctgcaatac cgaaacgatc ggttccagtt
     5341 tcgccagttg attgccaaac tgctgaacaa aacgcgcggc ttcctgggct tcatccagac
     5401 gttcgcggat ttcatcgacg cgatccgcca ggctgtcatc agccaacagg ttgagacgcg
     5461 gcagaatgcg gttcagcgcc gtaacgcctt ctttcgcctg ctcaaactga atacgctgct
     5521 gctggttatc attttcatga ttactcaacg cccgctccag ttcgacgcga cggctgttca
     5581 gttgacggat ttctgcttcc gggtcagact caaacgcaac cgccagatga ctgccgataa
     5641 agcggctgaa cgcctgatgc agacgctgag ttttctgtac atcaaaggag agcgtggcga
     5701 agcgttcgga aagcacttca cgctcggcat ggaggctttc aatacggctt tcacgcgcag
     5761 cacgaccaaa cagcggcact tccgggaaac gtgaataacg ccactgacga tcggcgattt
     5821 tcaccactac cgctttttcc agctcatcaa cgctgaacac gctgtcatcg aatgactgcg
     5881 gatctccttc gatcagatag agatcttccg ggcaatcggt caagccttcc aggtgttcag
     5941 ttacctgtga cagatctggc accacgatgg cgtggcgtga cgggccatac agcgctgaga
     6001 agtacggcgc atcttccagg ctaacgtcgt cataaatttc tgacagcagc acgccgccaa
     6061 aacgctccgc cagcgcgttc agacgctgat cttcagagcc gccaggctgg cttaaacgtt
     6121 cgatctcttc atcgacagcg tttttgcgcg cgcccacttc atcgcgttca acaatcgcct
     6181 ctcgctcacg ctccagcaac tgttgcagat attcggtgac gtcctggctg gaggtaaact
     6241 cttcaccgca ctgctcgctc aactggttga gactgttttg cgctgccagc caaaccggcg
     6301 cacgctgcat caaactctga atgcgagact gcagctgttc ctgttcctgg cgcagtgcca
     6361 tgcgctcttc acgggcgtta gacacgctat cggaaagaga ggcaatgcgc gcttccagct
     6421 cctgatgcag agcttccagt tcatcaatat caaaattctt gccctgacgt ttgcagaagt
     6481 ctgccagcag gcgttcggct tcttgctgct cgcgcagacg ctgttccagt tcacttaagc
     6541 gcatccgcaa cggttgaacc tgctctgcca ggtgacgctg atcgacccct tcgcgcaata
     6601 gttcgcgagc gacatcccac gcctcgttac gcgccagcgg gccgttgatt gccaccacca
     6661 gctgataagc ctgctcaaac tggctgtgcg cggtttgcgc catgctcatt ttctgctcaa
     6721 gagagagcat tttttcagtc gcttccagct ctttcgcctg gaaggtttcc agccattcgg
     6781 cggcgctgtc ggcggttaag tccggcagat ggcacagttc tttggcgcga ttaagcgcag
     6841 caatcgcctg gttatactgg atcgcgcgcg tttgctggac gtccagcgcc tgctggtagt
     6901 cagcaagctg gcttttcagc tcgtccactt ccagttcggc agcttccgcg cgagcctcgt
     6961 tctcttcctg gcgttcgatg gcttctgcca ccacttcgtt ttgctcttcc agacggatct
     7021 gcagctcatc gagatccgct tcgtagcgtt cgatcttctc ctgctgacgc agtgcggttt
     7081 gcaccaggtt caggtgatcg ctggccgcct gataatccgc ttccagatcg ccttcggcac
     7141 cgttgtgctc tgccagctca cgcgccatat cgacgtgttt gtactgctca gccgccagtt
     7201 gctgacgcga agtatgtaac tcgcgacgaa actccagggc tttgtcgaga tggacacggc
     7261 gctcgttggc gtgacgcatg tagtccgccg ccacgtagtt ggtagcttcg ctgatcagat
     7321 gcttaaacag gtcacggtcc gactgggtga cacgaatcgc ttccagcgtc atacggtttt
     7381 cacgcagcgc cgcttccatg tcctggaacg ctttacgcac gccgctgttt tccggcaaca
     7441 ggtagtcgcg cagagaacgg gtaatggcac tggagatccc gccatacagc gaagcttcga
     7501 tcagacgata gaatttgcta cggtcagatg cggagcgcag acgacgcgcg atgatgccca
     7561 gatcgaacat cagcgagtgg taatcagtaa tggagttgaa ctgtttaaac tgcacgcctt
     7621 ccatcgcctc gagcttgtct ttcagctcgt taagcggcag cacgcgcgcc tggcgttcgt
     7681 tcagggtttc ggtcaccagc tgtgtcggct gcaccgacat cggcagtccc tgaatggcaa
     7741 acggcttgat atccacttta cgatcgcgtc cggcaacctg ttgcagacgc acaccgacca
     7801 ccacgcgctg gtggcgcgag ttaatggtgt cgagcatcga ataacagaca cccgctttca
     7861 gcttaccgtg cagaccttta tcgcgcgaac cgctggtggc cccggcttcc gtagtgttac
     7921 ggaaatgcag cagggtcagg tcggggatca gcgccgtaac gaacgccgcc atggtggtgg
     7981 atttacccgc cccgttaccg ccagaaagcg tcgtgaccag ctcgtcaagg tcaaaagttc
     8041 gggcaaaaaa gccgttccag ttaatcagcg tcagtgagcg aaatttaccg cgttcaatca
     8101 ttattcttcc tctccgctat ctggctgatt ctcttcggtt tcatcgttga gttgcagatg
     8161 attttcaatc ggcattgctt cgccatcacg aatcaggcga cgctgtgctt cacggggatc
     8221 gtcgccagca cgcacatcgg ctccgaagcg gaacaccgat tcggtaatgc gaaacttgct
     8281 gctgtcgtgg cccataaacc acaccatgcc taaacgacgc agacggttga gcgaagaacg
     8341 tactttctcc tgcaacttct gacggtcaac gtctgaaccg gttgaacggt tgttcaccag
     8401 tttcagcagt tttgcttcat cggccagggt gagcagttcg tcgtacagtt cctgctgggt
     8461 gaaaatcccc tcattcgcca gccgttccgg gctgagatag agataacaga ggattttccc
     8521 gaccatcata tccagttccg acaagacgga acgagggatc agcgtggtgg aacgtgggcg
     8581 taaatagaag aacccttctg gtgcgcgaat aagctcaacg ttataacgcg cgtaaaactc
     8641 ttccagatat tcctgaaaat ccatcaggaa tgcatgatta tccagttcgt cgaggccaat
     8701 atggcggcct gaacgtaagg cgctgtccag cgccggaaat aacggattcg ccagcgcctg
     8761 cgccagctta accggcatca cttgttcaat atttgtcgat gacatgcgcc tgtaccttgg
     8821 ctccgtaatc attaatcggc tgccatttcg ctggcagtcc ggtgaaatct gcttgcgcta
     8881 cgccaagacg taccgcctga tcaataacaa tgcgcgcaac gtcaaagtga cgtgcacgcg
     8941 gatactgtga cagatattcg cgtaccacca gaccaagatc cagcggcact tgtctggttt
     9001 tgtacacggc aagttgttct tcgatgatcg ccgccagctg ttcgcggatc tcgttaaact
     9061 cttcgtattc cagatcctca ggaagttccc cagtcacttc ttcatcgcgc agtgccatct
     9121 cttcgtcacg catatccagc agacgatcgg cattggcata agttagcgcc cacggctcat
     9181 caaaataggt ttgtaccgac tgacgtaacc gctgagcaaa gacgcggttt ttatccatat
     9241 cgatcgcggt acgaataaat ttgtgtacgt ggcggtcgta gccaatccac aagtcgatgg
     9301 attgctggcc ccaactgata atacgatcga gtttgctctg cagatcgaac accagacgat
     9361 cgacgaaatg cagatcgtca tgggtcatcg tcgcatcctg aatgcgcaac agattagcct
     9421 gcaatttgtc gcctgccgct tccagcgtat cctgcaattc acgcagcgtt ccggaagttt
     9481 ccgaaagcaa caattcacag ctggaaatcg ccgcccgcca gtctttgttc agcaactggg
     9541 cgatatcgtc cttcacctgc tgctgctgtt cgtccatcag acgttgcgtc aggtcgatac
     9601 tgtcgaaaat ttctgctacc gaatatttca gtggcgcata gacattacgg tgccagtgaa
     9661 attcatcacc gccctcttcg gcggcatctg ctgcgcgttt gagctcaccc gccacaatcg
     9721 acaactgcat agaaagacgc agcgtagaaa actcgcgctg acggatgtag tagtcagtaa
     9781 tgccgatgcc gagcggcgtc agacggtaaa ttgcgttccc ttccgcctgc tcgctggtaa
     9841 agcggttcag cagacgttga cgcaccatgt cgttgatcgc gttattggcg cgcacgccga
     9901 tggtttcgct ggtttgctca aacgcatcac tcacatggcg gaatgcatcc accagctcgc
     9961 cttcactcat ctcaccatcc agacgctcgc cgttcagcgt ggcaaccgcc agcagaaaag
    10021 agagtcggtc taccggcagc gagatggaga agtcattttt tctggcccag gcaaccagtt
    10081 cggggactgt ctgggaaaat tcactcatac tttatccttg ctctgcggtt tgcgcgcggt
    10141 gacatgaata taacgcccca gggtaatata cggttcctga cggcaataac gcgtttctaa
    10201 ttcaagtaat gcttcatagc agtcgcgctg ctggtgcttc tcgcgcagat aatcatgaaa
    10261 cacgcgaacg cctgtcttac ccataatttg ccaaccagct tcttccagcc acagataaac
    10321 ctgcgccggg tcgcgtggat aatctggcga aagcgtccgt tttttctttt tcggcattcc
    10381 cgcctgcacg taatcaaaat tcccggcgac catgttatgc atcaacaaac catgcgcatt
    10441 gtagaacatt aacgacaaca cgccgcctgg acgcaacact gaccacaggg tctgcaatac
    10501 actgcgggga tcggccaccc actcgagcac cgcatggaac aatatcagat caacgggcgt
    10561 ttccaaatgc gaagcaacat cctgagcggc gcaatgtata aattgcatgt tgtcgctcac
    10621 accttttgct tctgccgcct gttttgcgcg gtcgatcatc tgcgcagaaa gatcgcataa
    10681 aatgacctga tgcccacgct cggccatttt gattgcggtc tgcccttctc caccgccagc
    10741 atccagcaca cgcagttttt gcgggcccat ttccgccagc acgcgatcga gatcctgcca
    10801 cagaatagcc tgtcgaagct gccctttggt ggtgccgtaa atgttacggg aaaacttttc
    10861 cgcaatatca tcaaaattgc gatcctgcat tgctttctcc accggttggc taagacgttt
    10921 tctgataaag acgctatttt gtcacagcca cggcgagaat gaacccgtct ggtgtaatat
    10981 cgctggcgat cccttgctat atggttaaaa aaggaaccag aaaggatgct ttttacactg
    11041 aaaaaagtga ttggcaatat gttgctgcca cttccgctga tgttgttaat tattggcgct
    11101 ggcctggcgc tgctgtggtt tagccgtttt caaaaaaccg ggaagatttt tattagcatt
    11161 gggtggctgg cactcttgtt attgagtttg caaccggtag ccgaccgcct gctgcgtccc
    11221 atcgaaagca cctatccgac atggaataat tcgcaaaaag tggactatat cgtggtgctc
    11281 ggaggtggtt atacctggaa cccgcagtgg gcaccgagct ctaatttaat caataacagt
    11341 ctgccacgcc tgaatgaagg tattcgctta tggcgcgaaa atccgggatc aaaactgatc
    11401 ttcacgggag gcgtagcaaa aaccaatacg gtgagtacag cggaagtagg tgccagagtt
    11461 gcgcaatcgc tgggcgtgcc gcgcgagcaa attatcaccc tggatttgcc aaaagatacc
    11521 gaagaagaag ctgcggcagt gaaacaggcg attggtgacg cccctttcct gctggtgacc
    11581 tccgcatcac atctgccgcg ggcgatgatc ttttttcagc aggaagggtt aaatccgcta
    11641 ccagccccgg cgaatcagtt ggccatcgac tcgccgctta atccgtggga acgggcaatc
    11701 ccttctccgg tctggctgat gcatagcgat cgtgttggct atgaaacgct tggccgcatc
    11761 tggcagtggc tgaaaggtcc gtcaggcgag ccacggcaag agtgattttg atgccagatc
    11821 gaagttgcgc cgactaaatc gtccggtatt aaccagttgc gcaacttcat cccataacac
    11881 atacaaccaa cgccgccaga tgaacgactc cgccactggc gcgcgttgca ggtaactcca
    11941 gagcaaatct tccgccagag aattatccat tagtcggaac agttcgtatt ctcgcggtgc
    12001 ccaaagcatt agtcccggcc cgaccatcgc cagtaactga tcgctgcgcg aatctttcag
    12061 catgctgcgt aaacagaagt taccgtgaat caacacacaa ttgtcgttaa agccttcaaa
    12121 taatgccggg agacattcgc gagtgcgaaa caggatccgc ttatcctgca tcgtcagacc
    12181 ggtgttattg aactgattga gcgtggtcca tagcacttca acatgttgcc ggtaccatga
    12241 gggccagaaa ttttcctgag tattgtcgac cgcgccgacg caaccgcgac tgtcctgacg
    12301 gtgccaggcc agtaaggctt caacgatttg gtctttgagt tgttcccagc gttctggtgt
    12361 tcgggctggt gcctccaccg aaacaccacg catccgctcc agtagcagga catcggggcc
    12421 ggggtgttct tcatgcgtca tcacaccata aatagtcggc atacggacag tcccacttct
    12481 tgccagcatg gtggttttcc atgccagttg tcgggctttt ccgggcgtac taaagcttct
    12541 tgccattaac ggcattgggt ttccctggct gtcatacaaa gcccacaacg ccgtatccgc
    12601 tttttcattg acgcactcaa tacgactgag tttttcgccc agtaaatggc ttaattcggc
    12661 acgcagctgt tccattcaga ttaccctcat aaaagctact gctttcataa tgagcgccaa
    12721 tataaaagat gtcacctgaa tgagatcaat tgttgaaaag aaaaattgag gaaaatcgta
    12781 acggtacgac actcctccca aaattggctg aagtgtcgtg aagtgaaatt agcgcatttc
    12841 agcgcgaacg cgctcaagat cttcaggggt atccacacct gtgccaggaa cttcctgagc
    12901 aacagcaaca tggatttttt cgccgtacca cagaacacga agctgctcta acatttcgat
    12961 gtgttctaac ggacttggct gccagttgac gtaacgacgg ataaagcctg cacggtagcc
    13021 ataaatacca agatgacgca ggaagttatc gccaacggtt tcaaggcctt ctgcaaaacg
    13081 atcacgatcc caaggaatgg tggcgcgaga gaagtacagt gcataccctt cagcgtcgag
    13141 aaccactttc accgcattcg ggttaaacgc ttcttccgca ttgtggattg gcaccgccag
    13201 agtcgccata cccacctgac gctgagcgag gttatcagca acctgacgaa tgattgtcgc
    13261 agggatcatc ggttcatcac cctgcacatt aacgatcacc gtgtcgtcgc tgaatgcgca
    13321 tttttcgaca acttccgcca ggcgttctgt ccctgactgg tgatcggcgc gcgtcataca
    13381 tacttcaccg ccagcggctt caacggcgcg ggcgacatcc tcatgatcgg ttgccacgat
    13441 gatgcgatcg gcacctgatt cgcgagcacg ttcaagaaca tgaacaatca tgggtttgcc
    13501 attaatatca accaatggtt taccgggcag acgcgttgac gcgtagcgcg cgggaataat
    13561 gaccacaaaa ctcatgattt actctcatca gcagtcagca cgcgggcttc cgtttccagc
    13621 aacactggaa tgccatcacg cagcgggaaa gccaggttgt ccagtttgca aataagttct
    13681 tgtttttctt ggttatacca aagttttccg ttgcaaaccg ggcaggcaat gatttcaagc
    13741 agacgatgat ccatagttcc tccagatgga tcgggttatg aatgcataaa tcttatcata
    13801 atcatttatg cgacggggtc tatttcccag ccagtgcggc aatcagtaaa gagaccttgc
    13861 gggcagcgtc ctaatgtcac ccgcgttgcc tgctgccagc tcgcgaaatc agtaatcgcc
    13921 tgacgtaacc ctttttgcag cattgtcgtt ggtttaatgc cttcctgtaa ccacagagag
    13981 ataacttcaa ggatgcctgt ctggcgatgc attttggcat ccattcgccc aactaattgc
    14041 ccacgatgta ataatggcag aacaaaatag ccatactggc gtttcggcgc tggggtatag
    14101 cactccagcc ggtagctaaa atcaaaaagc tgctctgcgc gtttgcgatc ccagacaaca
    14161 ggatcaaaag gcgaaagtac cgcgctgtgc gttgcagtga gctttccggc cagcgcacgc
    14221 tcgagtagcg gcagcaaatc agcatgcagc caaagattgc ccaatttttc aacatgcaca
    14281 gcaatgattt gctgctgttc agccctcgct tcgcgccacg ccgccagtgc cggacgtttc
    14341 agccgatagt aatctgccag ccactgttcg cggaatattc ccaggctacg cgcactgtta
    14401 tccagcatga tgatttctgc ttctgtttgc gaaacgagat cgcgctcatc atcccagtca
    14461 ggcatgacac ggtgggttaa atcataaacg cgctggaagt tgcgccgttc aatcaccatc
    14521 acctttccgg cagtaaataa accttccaga tgccgtttat gcggcttcca ttcccaccag
    14581 ccgcttgcac ctttacgagg atgctcaaaa tcggctgaac gtaccggccc cttatcatga
    14641 atatgctgaa ttaactgtgc aatttccgcc tcatgttcct gcatccaggc gtctttgtat
    14701 ttccagccca ttttttcagg tgccagcatg cggtggcgaa taagacgaaa gtcgctacgc
    14761 ggcataaagc aggcttcatg cgcccagtat tccattaatt cgccacgcgc cagagactca
    14821 tccagccact gggcaggata atttcccaga cgactgaaaa gcaccagata tggactacgg
    14881 gcaacaatat tgatggtatc gatttgcagc aaggacatgc gggagatcgt tgccggaata
    14941 tcctccaacg acgctcgacg gcggggtttg tttaacaggc cttgtgcggc aaggtgaaga
    15001 ttacgcgcat cagcaaagga gaggtgcggc agcgacattc atgactccat caatcgaacg
    15061 ctgccgcggc gtaactagtt gccagaagcc agcgaggtta gttgcgtaag cagtttcgct
    15121 ggttcatcac ctgaaagctg tgcgtctaca ggcaaatacc accaattttc ttctgcaaag
    15181 gcccggcatt tcaccgcatc tttttcagtc attaccagcg tttgcccggc gcttaccaac
    15241 gcactgacat ccgcatggtt caaagactga tgatcggcca gcggtacaca tttttccggt
    15301 tgtacgccac acatcttcag cgtggcaaaa aaacgcggcg gatgcccaat ccccgccatc
    15361 gccactacat gttcaagctg agcaacgtca caacgcgtac cggtacgtaa attcaccgcc
    15421 tgacccggca gcagatgcat ggggatttca ccgctgcgag ggacaccgcc gttgacgatt
    15481 accgcatcaa ccgactttaa gcgccccgct cgctcacgca ttggccccgc cggcaaccac
    15541 cagccattgc caaagcgacg cacaccatca ataacgacaa tttccacatc acgcgccaga
    15601 cggtaatgct gtaaaccgtc gtcggttacg atcatctgca catcagggtg ttgcgccaga
    15661 atggctttta ccgcatcaga acgaacggga gaaaccgcaa caggcgcatc agtgcgttga
    15721 taaatcaaca caggttcatc acccgcctgt gctgttgtgg tatctgccga caataacagc
    15781 ggataagatt cagccttacc accatatccc cgcgatacga cccccacgcg aataccgcgc
    15841 tgttgcaact gttccaccag ccagacaacg accggggttt ttccgttgcc gcctgcggtg
    15901 agattaccaa ccacgacaac cggtacgggg gcacgccagg cgcgcttcag ttttagttta
    15961 tagcaaagac ggatcgcgcc acttaccagg ccatacaacc aggagagtgg cagcaatagc
    16021 cgccacaaag gggattcacc agaccagatt ttttcgatca ttggccaaac tgcattttgt
    16081 gaagttgcgc gtaaacgccg cggtgctcaa gcaaatcgtt atgcgtaccg cgttccacaa
    16141 tgacaccatc ctcgacgacc acgatttcgt ctgccttttc aatggtagac aagcggtggg
    16201 caatcaccag agaggtacgg tttttctgca actcatccag tgccgcctga atcgcacgtt
    16261 cggattcggt atccagagcc gaggtagctt catccagaat cagaatcggg ctatcacgca
    16321 gcaaggctcg agcgatagca atacgctgac gctgaccgcc agagagcagc acgccgtttt
    16381 caccaatcac tgtatcgaga ccgttatcca tcttattgat gaagtccatg gcgtaggcca
    16441 tacgcgccgc ttcttcaatt tgctcacggc tgtactgttc agtccgtgcg taagcaatgt
    16501 tgttagcaac cgtatcgtta aacagatgga cattctgcga caccagagca acctggttac
    16561 gtaacgacgc cagggtatac tcgcgcagat cgtgaccatc catcaggatt tcgccttcat
    16621 caatatcgta aaaacgagtg atcaggctgg cgatggttga tttacctgaa ccagagcgtc
    16681 caaccagagc aaccgtcttc cctgccggaa ttttcaggtt gatgttacgc aatgcaggta
    16741 cgtcacgtcc cggataagta aaggtgacat tgcggaattc cacgtcgcca gtcgcacgct
    16801 cgatcacgcg cttaccttcg tctttctcct gctcactgtc cagaatggta aacagcgtct
    16861 gacaagccgc cataccgcgc tggaactggg cgttaacgtt ggtcagtgat ttcagcggac
    16921 gcatcagtgc aatcattgaa gagaaaacaa cggtaatcgt accggcagtc aggctatcca
    16981 tgacacttgg gaagctcgcc gcatacagaa caaacgccag cgccaaagag gcgatcagct
    17041 gaatgatcgg atcagagatg gaagaggctg aaaccatttt catcccctga agacgcattc
    17101 ggttgctgac tttatcaaag cgtttcgttt ccacttcctg accaccgaaa atcaatactt
    17161 ctttgtggcc cttcagcatt tgttcggcgc tggtggtcac ctgccccatg gtgttctgca
    17221 tgtttttact gatgttgcga aaacgcttcg atactacgcg aatcgcaatc gaaacaatcg
    17281 gtgccagcac aatcaaaatg atcgacagtt gccaactgta atagaacatc atgatgaaca
    17341 ggccgatgat tgacgcacct tcacgcacaa ccgttatcag tgcgccggaa gaagaagaag
    17401 caacctgttc ggaatcgtag gtgatacgtg acaacagcgt ccccgttgac tgtttgtcaa
    17461 aaaatgaaac cggcattccc atcatgtgac caaacaggcg gcgacgcatg gtcattacca
    17521 cctttcctga tacccaggag atacagtagc tggagacata gctggtgata ccacgtaaaa
    17581 tcatcagccc gatcaccacc agcggcatcc acaccagcac ggagcgatct gttttaccaa
    17641 agccatcatc aagaagtggc ttaaggagcg ataacatgaa ggtatcgctg gctgcgttga
    17701 ggattaacgc tacgcccgcc acgatcagac ccgctttaaa aggcgcaatg gttggccaca
    17761 gtcggcggaa cgtctgccac gtagagagat ctttgtcgtt atgcattcaa aaaaccagca
    17821 tttgttgaaa tagccgcata ttctacccgt tatccactgg cacgccaaac cactgatgat
    17881 accaacgagg taaaatttgc tctctgaggc tgctaatccg ccagccttgc gctgaaaaat
    17941 tgaccgtcac ttgaccctga tgtggagtat caagccattg atatccttgc tgttgatagc
    18001 gatgcttaac tttattagag ggcaatcgcc atgcgttata gcgcgatgcc gatgcgagtg
    18061 ccacttttcc attcactcgc tgaattaatg gcaacgatga tgaggtatta ctgccatggt
    18121 gaggtacctg aagcaatgtt gtctgcactt gctgccagta acggcttagc atcttttgtt
    18181 cagcggggac ttcaatatca ccggttagaa gaatgctatt cgtcccgtca tcaaccttaa
    18241 ccacacagga atggttattt cctttatcgt tgctagcttg taaaggccag tgcacgctga
    18301 aacgcaatcc ttgccattgc cacgcttcgc cacgcacaca gggctgatga tgttcccagt
    18361 ttaacggact tctgatccat aacatcggcc atgtgtgcaa tattgagtcc agccctcccc
    18421 ggtgatccag atgttcatga ctcagaataa cgccttccgg ttcaagatta tgccagtgga
    18481 gccaggggat aatcagttgt tgcccactat ccccttcggg ccaggccagt cctgtgtcat
    18541 agagaatcgc tttgccgttt ctggctatca ccattgccag cccttgcccg acatcaagca
    18601 tgtacacctg ccactcgtca ggtcgaggtt tttgccacag cggccagcac atcagcaagc
    18661 ctccagccac acacattgct ggcaacgttc gccaggcgtt taatcgccat accaccagta
    18721 agaaccatgg ggaaaatgat agccattgcc aacattcagc aatgttgatc cacccttccg
    18781 gcaaactctt taacccccag aaaagtaaag ccaaagaccg gtcggcaaga aaccataacc
    18841 cttgctccag gattaacggc ccgcttaaat gcacaaccat cgcggcgagg atcaacggaa
    18901 ccgtgataaa tgtcaccaag ggaattgcta atagatttgc aataaacgag gtcagactaa
    18961 tgccatgaaa tatgacgatt tgcacgggca taagcagaag tgtgattccc agttgcagat
    19021 ggatgaggga aacaactgca cgcaataccg gcggcagttg ccactcagga cagggaaacc
    19081 actgatacca aaatatcaat gccgcgaccg cagcggcaga gagccataaa ctttgcgaga
    19141 gaatggcaac aggatccatc agcaaaattg ccgccagaca acatatccat acatcccagc
    19201 cactccactg tcgcccactt aacttaagca ttccccacgt agcaagcgcc accatggtac
    19261 gcaatgcagg aggttgcatt cccgtcaacc aggcataaaa agcagcacag cagattccgc
    19321 caattaatgg tatttgccag tggatccagc gcccaggcag aaaaatttgt ccactgcgaa
    19381 tgagtccggc agccagcaac gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat
    19441 gcgccgttcc agtatcgcgc atgatatttt tgatttcttt ggggacggat aaccgttccc
    19501 ccatacctaa accaagaata accgcattcc acggataggg ttgcagcgtt gtttgtagtg
    19561 acgccagata ctgtgcacgc aggctacaat tcggttcaat gacacttgcc tgcagaaaac
    19621 ggccggtgag cggctgatgc tgggcaatgg cataacgctg gctatcaaag ccgccatcat
    19681 taagttggcc atgaactgca cgaactttga gtttcattga ccattgttgt ccagcacaaa
    19741 ccgcttgcgg aagatattcg ccatacatca cgagacctga cgcagggaat atacgtcgac
    19801 cttgtagatg agtaatttga ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa
    19861 caattgcatc ctgcgtcgcg ccagttaagg tttctcctgc ccacaaaatt tgctttgctg
    19921 ataatatgcc ccaaacgaaa aacagcaacg tcagcgcgat ataacggacg gttttaacag
    19981 ggataaatgc cagtacgcag gcgaagagag tcagaaacgc aagggttaat gtcccaggca
    20041 attggggcaa aatcagcaac ggaaaaattc cgctaattat gcatacaccg actgtcgtta
    20101 ttttcatgca cccctccctg tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt
    20161 gggaattttg cgttccccga ggcacattca gaagaattta acgaaagggt gaaaactgct
    20221 caaaactctg cgaggcagct tccagttcct gattagagaa atgaaaaaag cacccgacag
    20281 gtgcttttct cgcgttcaag tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg
    20341 cagttcttta ccaggtttaa agtgaggaac gtattttcct tccagttcta ctttatcgcc
    20401 agtcttcgga ttacgtccgg tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc
    20461 gcggatttca atacgctcgc cctgcgcaag agtcgaggcc atatgctcca gcatctcttt
    20521 tactgcatct tcaaccgtct tggcgggaat gtgcgattgc tgggtggcaa gtctttctat
    20581 caattctgac ttggtcatga ttcctccggt tccttaaagg caaattaagc ggctgctgtt
    20641 gctgcattga ttaagggcgg ccgtagccgc ccttagtgct tgattacagg acgaaacctg
    20701 caatctgtca agtaaactca acaaacttcg gaataaaaat cccgaagagt cagagaatta
    20761 ctcgccttta gctgctttga aagcttcagc cattgcgttg ttggagaagt ttgcatcttc
    20821 ctgtttgtta acagttgcga ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga
    20881 caggctgatt gcgcggtttt tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc
    20941 aacgctcaga accagggtag cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag
    21001 gtaaccttca acgccgtcag ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac
    21061 tttaccggtt acgatagcgc ctttcttgtt cagagcaacc cagttgttga acggatcttc
    21121 tgcgagctgt ttaacgccca gggagatacg ttcacgttct gcgtcaacct gcagaacaac
    21181 tgcagcgatt tcgtcgcctt ttttgtattc acgaactgct tcttcgcctg caacgttcca
    21241 ggagatgtca gacaggtgaa ccaggccgtc gatgccgccg tccaggccga tgaagatacc
    21301 gaagtcagtg atagacttga ttttaccttc aacacggtcg cccttgttgt gggtttccgc
    21361 gaactgctgc cacgggttag ctttgcactg tttcagaccc agggagatac gacgacgttc
    21421 ttcgtcgata tccagaacca taacttccac tacatcgcca acgttaacaa ctttggacgg
    21481 gtggatgttt ttgttggtcc aatccatttc ggaaacgtgt accaggcctt caacgccttc
    21541 ttcgatttca acgaagcagc cgtagtcggt caggttggtc acgcgaccag tcagtttggt
    21601 accttccgga taacgtttag cgatagctac ccacggatct tcgcccaact gtttcaggcc
    21661 cagggataca cgggtacgtt cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc
    21721 cacgttgacg atttcgctcg gatgcttaac gcgtttccag gccatgtcag tgatgtgcag
    21781 caggccgtca acgccgccca gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat
    21841 acctttaact tccatgcctt cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt
    21901 ttcggattcg ataacggcac gacgagaaac aacaacgttg ttgcgcttct gatccagctt
    21961 gattacttta aattcaagct ctttgccttc caggtgcaga gtgtcacgca ccggacgaac
    22021 gtctaccaga gaacctggca ggaacgcacg aataccgttc agctcaacag tgaagccgcc
    22081 cttaactttg ccgttgataa caccggtaac agtttcagca tcttcgtaag ctttttccag
    22141 cgtgatccag gcttcgtgac gtttagcttt ctcacgggac agcagagttt caccgaagcc
    22201 gtcttctact gcgtccagag caacgtcaac ttcatcacct acctggattt ccagctcgcc
    22261 ctgggcgttt ttgaactgct cagccgggat ggcggactca gatttcagac cagcgtcaac
    22321 cagtactacg tctttgtcga tagcaacaac aacgccacga acgatagaac ccgggcgggt
    22381 ttcgatttct tttaaggact cttcaaagag ttgagcaaaa gattcagtca tgtttaatct
    22441 tcaggtttat atttaacgtc cacctggctc catgccggat ggggttgttt aacatacccg
    22501 ctgtcattcc attgcaacgg gggtactgca aattcggtcg cttatgcgag agccaatttc
    22561 tggcgcgcgt attgtagcgc tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc
    22621 aacactaaag catcggctgc cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg
    22681 tcgcgttctt tgatctcggc caaaaggcgc tcaaagttaa cactaaagcc cttctcctgc
    22741 aactgtagca tgcggcgatg cgcacgttct tccgaggagg cgtcaaggaa aattttcact
    22801 ggcgcatcag ggaataccac cgttcccatg tcgcggccat cggcaatcag gccgggtaat
    22861 tcgcgaaacg cgcgttggcg acgcaataat gcttcacgaa cgcgtgggaa tgccgcgact
    22921 tgtgaagctg cattcgccac ttcctgagta cgaatttcgc cgctgacatc ttccccttcg
    22981 aggatcactt ccagattgcc attggttgac acaaaacgta catccagatg ggatgccagc
    23041 ggtaccagcg catcttctga cgcaacatca acatggtgat gtaatgccgc caatgccagt
    23101 acgcgataaa ttgcacccga atccagcaga tgccattgca atgcttccgc catagcctta
    23161 cacaaggtgc ctttccctgc accgcttggg ccatcaatgg taataaccgg ggcaattgcc
    23221 gtcatcttta tctccttaaa acaggcgtac cgttaacata accgcgcgca ttatacgcgc
    23281 cgacgccaca aatcgttaac gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg
    23341 atttattgcg caattatagc gggctggcag aagaccagcc cgaaagatga caatgattta
    23401 cttaccagag gcgatacgat cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga
    23461 gtcaaacatg gactgggtgc gaccgccatc cagactagcc agtttttcaa agctgccaac
    23521 cagcccctgg gtgctgatgc cacgtttctt caacagatca aaggagaaat catctgcttc
    23581 ggactcttta tcacgggagt atttagcatt aatagcgcct tctgcgatat cacccaattg
    23641 tgagcgggaa agctgggaag ccacaccgct ggtagctgaa atggcatcgc gtgcggcaac
    23701 gatcgcataa gaagctttca tttcagccag cgagtgaccc aacgcgacgt ggcccagttc
    23761 atggcccaga acaccttcaa tttcgttgtc attcatcatg tccatcaggc cactgtagac
    23821 acggacacag ccgttcgcca tcgcccatgc gttgacgtcg ctggttatat aaaccttata
    23881 gttgaccggc gtgccattaa tattgttacc caatgctttg gcgatttttg ccagacgttt
    23941 accgtacttg ctggatttac ttgcgacttg attgccgctg tccatttcgg cacagccctg
    24001 attggcaatc gcttttgcat cggcatcgct taatgttgct gctttatagg catttaaacc
    24061 ggagctgata gccatattgg tatcaatacc gtgggtattt tgacaccctg tcagtaatgc
    24121 tgcagaggtc gcaatcgcca gcagtaattt agtattcttc atgtgaatca tccgttgatt
    24181 aatcatatat aaatattatg tatcaacaac ttacattgac aagcgacgct accattaatc
    24241 aaaatgactc acaaggtccg aaaaaaaacg ccgacaatgt gccgacgttt tgataaagaa
    24301 agatttggct atttattgcc cgttgttcat tcaggctgcc tggctaatcc gcgccaactg
    24361 ctcgaaataa tccggaaatg ttttggccgt acatttggga tcaagaatcg tcactggtgt
    24421 atctgacaac gccaccagcg agaaacacat cgccatccgg tgatcattgt acgtcgcgat
    24481 ctcggcaaag ttcagttttt ccggtggagt gatacgaatg taatcgtgcc cctcttctac
    24541 ttccgcaccg actttacgca gttctgttgc catcgcaaac aggcgatcgg tttctttaac
    24601 acgccagtta tagatattgc gcagcgtggt ggtgcctttt gcaaataacg ccgccgtggc
    24661 aatggtcatc gccgcatcgg gaatatggtt catatccata tcaatagcgt tcagttcacc
    24721 acgcgtgcag gaaatataat catcgcccca gcaaatggtc gcgcccattt tttccagcac
    24781 atcagcaaag cgaatatcac cctgcatact gttacgtcca ataccggtca cttttacagt
    24841 gccgcctttg attgctgctg ctgccagaaa gtaagaagcc gaagatgcat cgccttcgac
    24901 caaataagta cccggagact gataagactg cccgcctttt acgacaaatt gttgatagtg
    24961 ctgattttca atttcaacac caaacgtctt catcagattg agtgtgatgt cgatataagg
    25021 tttagaaacc agatcgcctt taatacgaat caccgtatct tccggcgcaa gaggcgcagt
    25081 cattaacagt gcggtgagga attggctgga aacggagcca tcaacgtcaa cgttgccgcc
    25141 agtaaagccg ccctgtaaac gcaacggcgg ataattttct tgttccaggt aagtgatctt
    25201 cgccccgccc aggcgcagcg catccaccag atgaccaatc gggcgttctt tcatacgcgg
    25261 ctcaccggtc agcacaatat cattgctacc cagacaaaga gctgccgcca gcggacgcat
    25321 tgccgttccg gcgttaccga ggaacaactc cagggcacct tctgcgtgta atggaccgcc
    25381 gttaccgata atttcgcaac gcgtacgatc ggctgaaagc gtatagctta cccctaacgc
    25441 tgttaatgca ttcagcatat ggcgcacgtc atcgctatcc agcagattgg ttaatactgt
    25501 tttgccgtgt gctaatgccg ccagcaataa agcgcggtta gaaacgctct tggaaccggg
    25561 cagattaata gtgccatcga cacgagcgat gggttgtaac gtcagggatt ccatgaactc
    25621 aactctctac aacagaaata aaaaccccac aggctggctg tggggattaa gcaaaatttc
    25681 ggcattaacc gtgacggcgt tcgaactcaa ccatgaagtc tgtcagcgct ttaacgcctt
    25741 ccagcggcat ggcgttataa atagaagcgc gcattccgcc gaccacacgg tgacctttca
    25801 gtgcatgaag gccagcagca aaagactctt caaggaacaa tttgtcaagc gcactgtccg
    25861 ccaactggaa cggcacgttc atccgcgaac ggttagcttt cgccacgtca ttgcggtaga
    25921 aatcgctgtt atcaatcacc ccatatagca gttctgcttt ttgctgattg attttatcca
    25981 tttcagctac accgccgttc gctttcagcc atttaaagac cagaccagat agataccagg
    26041 caaatgtcgg cggcgtgtta aacatggagc cgttatcgtt gaggatggaa taatccagaa
    26101 tcgacggaca cgcgatattc gctttgccca gcaaatcttc acgaacgatg acgattgtca
    26161 ggccagccgg gccgatattt ttctgcgcgc cagcgtaaat cacgccataa cggctgacgt
    26221 caatcggacg ggaaagaatg gttgaagaga agtcggcggc gaccaccaca tctttgccga
    26281 agtctggcgt ttcgtcgatg gcgataccgt cgatggtttc attcgggcag taatgcatat
    26341 aagcagcatt atcagagagt tgccattcac gcattggctt aaccgcgcgc agaccatcaa
    26401 cagtcacttt ggcgtcaaag acattaggcg tgcagtattt tttcgcttct ttaatggcac
    26461 ttgccgccca gtaaccggca tcaacataat ctgcggtggt tttatcaccg agaatattca
    26521 gcggtaccgc agcaaactga ccgcgaccac cgccatggca gaataatacc ttgtagttgg
    26581 aggggacatt aagaagatcg cgaaaatcct tctcggcttc ctctgcaacc tgaatgaact
    26641 ctttgccacg gtgactcact tccatcaccg acgtaccaag accgttccag tcgcgcagtt
    26701 cctgttgagc ctgtttaagc acctctgccg gtagcattgc cggaccagaa ctaaaattga
    26761 agatttgagc catttcccct caccacgttg cgttgctatt aacccgcggt caatcgattg
    26821 cgaccacagg taatacatta ttcctgtggc tatcggtttt atcattcagt gacacacatc
    26881 gcaatgagta aaaccgttga gggcaaaaat gtggcttgca tcacacaaaa gaatctctcg
    26941 gattattagc aaaaagccga tgtttttact gtcaggtcac ccgttttgcc cgacttgcct
    27001 tcgtccattc tggatttgca cagcgcggac ataattgttt ttcccccgct ttcatatgaa
    27061 tgatttcact acaacgaata caggcatagt ccgccgactc cggcaccact ttgtaacgct
    27121 gcgtacaatc actgggtaaa ataagtaaac ccggtttcac cttgtcatct tcaaagaaat
    27181 agacactgat ttgcccgttg gtttcgagaa tcgccagacg tacctgcccc agctgctcca
    27241 cgccacgcaa tcgtagctcc ataaagaact caaattccgt catgttggag ttattgagtt
    27301 tcgaccaggc cagctcgcca tcttcaataa taacaaccgg cttaccttcc agtagatctt
    27361 ccagtttttc actgtgcgcc atcaaccaca ttaccaggcg gtataacaac gccagagtga
    27421 taaagacgat aagtaccggc accatcggca catcatcata aaacgccaca tctcccgccg
    27481 ctgaacccag cgtgagaatg attaacactt caaacagcga catctgtcgc acaccgcgtc
    27541 ttccggtcat tttgaggaac aaaaaaacca gtacaaaggt ataaagacta cgtagtgcaa
    27601 cttctccaag gaaatcaaaa ggcactttat caaatgccat acggtggaga tcgaacgcct
    27661 tcatttttat gctcttatcg tggtttatcc gctaaaagca tagtcaacag ttcatcggca
    27721 tggcggcggt gatagcgcct ttgagataaa acccgtatta ttgcgcgctt tccgtacgac
    27781 taaagtgatt ttcatgacgc aaacatttat ccccggcaaa gatgccgctc tggaagattc
    27841 catcgctcgc ttccagcaaa aactttcaga cctcggcttt cagattgaag aggcctcctg
    27901 gctgaatcct gtgcctaacg tctggtctgt acatattcgc gacaaagagt gcgcactgtg
    27961 ttttaccaac ggaaaaggcg caaccaaaaa agcggcgctg gcttctgcac ttggtgaata
    28021 tttcgagcgt ctctcaacca actacttttt tgctgatttc tggctgggcg aaaccatcgc
    28081 caacggtccg ttcgtgcatt atcccaacga aaaatggttc ccactgaccg aaaatgacga
    28141 tgtgccagaa ggactactcg atgaccgtct gcgcgcgttt tatgatccgg agaatgaact
    28201 gaccggcagc atgctgattg acctacaatc cggtaacgaa gatcgtggta tttgcggcct
    28261 gccgtttacg cgtcagtccg acaatcagac cgtttatatt ccgatgaata tcattggtaa
    28321 cctgtacgtc tccaacggta tgtccgcagg taatacccgc aacgaagcac gcgttcaggg
    28381 attgtctgaa gttttcgaac gctacgtgaa aaaccgcatt attgctgaaa gcatcagcct
    28441 gccagagatc ccggcagacg tgctggcgcg ttacccagca gtagttgaag ccatcgaaac
    28501 gctggaagcg gagggtttcc caatcttcgc ttatgacggt tcgcttggcg gccagtatcc
    28561 ggtgatttgc gtggtactgt tcaatcctgc taacggtacc tgctttgcct ctttcggtgc
    28621 gcatcctgat tttggcgtag cactggaacg taccgtgacc gagctgctgc aaggtcgtgg
    28681 cctgaaagat ttggatgtgt ttactccgcc aaccttcgat gatgaagaag tcgctgaaca
    28741 taccaacctc gaaacgcact ttatcgattc cagcggttta atctcctggg acctgttcaa
    28801 gcaggatgcc gattatccgt ttgtggactg gaatttctcc ggcaccacgg aagaagagtt
    28861 cgccacgctg atggctatct tcaaaaagga agataaagaa gtttatattg ccgattacga
    28921 gcatctgggt gtctacgctt gccgcattat cgtgcctggc atgtccgata tttatccggc
    28981 tgaagatctg tggctcgcga ataacagtat gggcagccat ttacgtgaaa cgattctttc
    29041 gctaccaggc agcgagtggg aaaaagaaga ttacctgaac ctcatcgagc aactggatga
    29101 agaaggtttt gatgacttta cccgcgtgcg tgagctgttg ggtctggcga ccgggtcgga
    29161 taacggttgg tacaccctgc gtatcggtga attaaaagcc atgctggcgc tggctggtgg
    29221 cgatctggaa caggctctgg tctggaccga atggacgatg gagtttaact catcagtatt
    29281 tagtccggaa cgcgccaact attatcgctg cctgcaaacg ttgttattac tggcacagga
    29341 agaagatcgc cagccgctgc aatatctgaa tgcgtttgtt cgcatgtacg gcgcagatgc
    29401 cgtagaagcc gccagtgcgg caatgagcgg cgaagcggcg ttttacggcc tgcaaccggt
    29461 agatagcgat ctgcacgcgt ttgctgcaca tcagtcgttg ttgaaggcct acgaaaagct
    29521 gcagcgcgcc aaagcagcat tctgggcaaa ataaaatcaa atagcctacg caatgtaggc
    29581 ttaatgatta gtctgagtta tattacgggg cgttttttta
//
LOCUS       DAZGSP010000062        29204 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZGSP010000062 DAZGSP010000000
VERSION     DAZGSP010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.062"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            297..1163
                     /gene="folD"
                     /locus_tag="ACHK74_002979"
     CDS             297..1163
                     /gene="folD"
                     /locus_tag="ACHK74_002979"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFP0221756.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            1165..1377
                     /gene="ybcJ"
                     /locus_tag="ACHK74_002980"
     CDS             1165..1377
                     /gene="ybcJ"
                     /locus_tag="ACHK74_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFP0221757.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1485..2006
                     /gene="ybcI"
                     /locus_tag="ACHK74_002981"
     CDS             1485..2006
                     /gene="ybcI"
                     /locus_tag="ACHK74_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0221758.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(2042..3427)
                     /gene="cysS"
                     /locus_tag="ACHK74_002982"
     CDS             complement(2042..3427)
                     /gene="cysS"
                     /locus_tag="ACHK74_002982"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFP0221759.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3601..4095
                     /gene="ppiB"
                     /locus_tag="ACHK74_002983"
     CDS             3601..4095
                     /gene="ppiB"
                     /locus_tag="ACHK74_002983"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFP0221760.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4098..4820
                     /gene="lpxH"
                     /locus_tag="ACHK74_002984"
     CDS             4098..4820
                     /gene="lpxH"
                     /locus_tag="ACHK74_002984"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFP0221761.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4938..5447
                     /gene="purE"
                     /locus_tag="ACHK74_002985"
     CDS             4938..5447
                     /gene="purE"
                     /locus_tag="ACHK74_002985"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFP0221762.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPYHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATYDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5444..6511
                     /gene="purK"
                     /locus_tag="ACHK74_002986"
     CDS             5444..6511
                     /gene="purK"
                     /locus_tag="ACHK74_002986"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFP0221763.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6648..7541)
                     /gene="ybcF"
                     /locus_tag="ACHK74_002987"
     CDS             complement(6648..7541)
                     /gene="ybcF"
                     /locus_tag="ACHK74_002987"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP0221764.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7538..8353)
                     /gene="ylbF"
                     /locus_tag="ACHK74_002988"
     CDS             complement(7538..8353)
                     /gene="ylbF"
                     /locus_tag="ACHK74_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP0221765.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8364..9623)
                     /gene="ylbE"
                     /locus_tag="ACHK74_002989"
     CDS             complement(8364..9623)
                     /gene="ylbE"
                     /locus_tag="ACHK74_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP0221766.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9633..11300)
                     /gene="fdrA"
                     /locus_tag="ACHK74_002990"
     CDS             complement(9633..11300)
                     /gene="fdrA"
                     /locus_tag="ACHK74_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP0221767.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELTNQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSSATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11617..12666
                     /gene="allD"
                     /locus_tag="ACHK74_002991"
     CDS             11617..12666
                     /gene="allD"
                     /locus_tag="ACHK74_002991"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFP0221768.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLHARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFFSS
                     KLFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12688..13923
                     /gene="allC"
                     /locus_tag="ACHK74_002992"
     CDS             12688..13923
                     /gene="allC"
                     /locus_tag="ACHK74_002992"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFP0221769.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            13934..14719
                     /gene="allE"
                     /locus_tag="ACHK74_002993"
     CDS             13934..14719
                     /gene="allE"
                     /locus_tag="ACHK74_002993"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFP0221770.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(14947..16092)
                     /gene="glxK"
                     /locus_tag="ACHK74_002994"
     CDS             complement(14947..16092)
                     /gene="glxK"
                     /locus_tag="ACHK74_002994"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFP0221771.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(16114..17415)
                     /gene="ybbY"
                     /locus_tag="ACHK74_002995"
     CDS             complement(16114..17415)
                     /gene="ybbY"
                     /locus_tag="ACHK74_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFP0221772.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLLTVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(17472..18833)
                     /gene="allB"
                     /locus_tag="ACHK74_002996"
     CDS             complement(17472..18833)
                     /gene="allB"
                     /locus_tag="ACHK74_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFP0221773.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFIGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(18893..20347)
                     /gene="ybbW"
                     /locus_tag="ACHK74_002997"
     CDS             complement(18893..20347)
                     /gene="ybbW"
                     /locus_tag="ACHK74_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFP0221774.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLNYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGKINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(20516..21394)
                     /gene="glxR"
                     /locus_tag="ACHK74_002998"
     CDS             complement(20516..21394)
                     /gene="glxR"
                     /locus_tag="ACHK74_002998"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP0221775.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIADMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(21494..22270)
                     /gene="hyi"
                     /locus_tag="ACHK74_002999"
     CDS             complement(21494..22270)
                     /gene="hyi"
                     /locus_tag="ACHK74_002999"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFP0221776.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(22283..24064)
                     /gene="gcl"
                     /locus_tag="ACHK74_003000"
     CDS             complement(22283..24064)
                     /gene="gcl"
                     /locus_tag="ACHK74_003000"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFP0221777.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKVALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LHNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(24154..24969)
                     /gene="allR"
                     /locus_tag="ACHK74_003001"
     CDS             complement(24154..24969)
                     /gene="allR"
                     /locus_tag="ACHK74_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFP0221778.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCL
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(25047..25529)
                     /gene="allA"
                     /locus_tag="ACHK74_003002"
     CDS             complement(25047..25529)
                     /gene="allA"
                     /locus_tag="ACHK74_003002"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFP0221779.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EIREQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            25759..26685
                     /gene="allS"
                     /locus_tag="ACHK74_003003"
     CDS             25759..26685
                     /gene="allS"
                     /locus_tag="ACHK74_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFP0221780.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            26754..27848
                     /gene="mnmH"
                     /locus_tag="ACHK74_003004"
     CDS             26754..27848
                     /gene="mnmH"
                     /locus_tag="ACHK74_003004"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFP0221781.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLAPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            27964..28329
                     /gene="ylbG"
                     /locus_tag="ACHK74_003005"
                     /pseudo
     CDS             27964..28329
                     /gene="ylbG"
                     /locus_tag="ACHK74_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(29096..29204)
                     /locus_tag="ACHK74_003006"
                     /pseudo
     CDS             complement(29096..29204)
                     /locus_tag="ACHK74_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7220 a         7884 c         7220 g         6880 t
ORIGIN      
        1 aaaaatatag acctgaagcg gtaaattacc aggctgaaaa ttctttttat attgtcaggt
       61 atttcttaaa ttatcttaat ccttaaacaa ggaaataaat cagttccaga tttacaacgc
      121 catcatggac gaaaaatgaa gctttcaatc tcagcgacct cagcgacggt gcgcctaacc
      181 ttcgcaagag gtcgcttcac gcgataaatc tgaaacgaaa cctgacagcg cgccccgctt
      241 ctgacaaaat agacgcatcc ccttcgacct acgtaacaga tggaatcctc tctctgatgg
      301 cagcaaagat tattgacggt aaaacgattg cgcagcaggt gcgctctgaa gttgctcaaa
      361 aagttcaggc gcgtattgca gccggactgc gggcaccagg actggccgtt gtgctggtgg
      421 gtagtaaccc tgcatcgcaa atttatgtcg caagcaaacg caaggcttgt gaagaagtcg
      481 ggttcgtctc ccgctcttat gacctcccgg aaaccaccag cgaagcggag ctgctggagc
      541 ttatcgatac gctgaatgcc gacaacacca tcgatggcat tctggttcaa ctgccgttac
      601 cggcgggtat tgataacgtc aaagtgctgg aacgtattca tccggacaaa gacgtggacg
      661 gtttccatcc ttacaacgtc ggtcgtctgt gccagcgcgc gccgcgtctg cgtccctgca
      721 ccccgcgcgg tatcgtcacg ctgcttgagc gttacaacat tgataccttc ggcctcaacg
      781 ccgtggtgat tggcgcatcg aatatcgtcg gtcgcccaat gagcatggaa ctgttgctcg
      841 caggttgcac cactaccgtg actcaccgct tcactaaaaa tctgcgtcat cacgtagaaa
      901 atgccgatct gttgatcgtt gccgttggca agccaggctt tattcccggt gactggatca
      961 aagaaggcgc aattgtgatt gatgtcggca tcaaccgtct ggaaaatggc aaagttgtgg
     1021 gcgacgtcgt gtttgaagac gcggctaaac gcgcctcata cattacgcct gttcccggcg
     1081 gcgttggccc gatgacggtt gccacgctga ttgaaaacac gctacaggcg tgcgttgaat
     1141 atcatgatcc acaggatgag taacatggcg acattttctt taggtaaaca tccgcacgtt
     1201 gagctgtgcg acttgctgaa actggaaggc tggagcgaaa gcggcgcgca ggcgaaaatc
     1261 gcgattgccg aaggccaggt gaaagtcgac ggtgcggttg aaacgcgcaa acgctgcaaa
     1321 atcgtcgccg gtcagacagt gagttttgca ggtcacagcg tacaggttgt tgcctgatcc
     1381 ctttcccggc ctgtatcaac aggccggttc tcttttcaga cttttgccct ctacgccccg
     1441 ttcatccatg ctgatagcat cgacattttg ccgaagcgac catcatgcca accgttatta
     1501 cgcacgccgc cgttccccta tgcattggtt tagggttggg ttcgaaagtc atccctccgc
     1561 gtctgttatt tgccggaatc atcctggcga tgctgcccga cgccgacgta ttgtcgttta
     1621 aatttggcgt tgcttacggc aatgtttttg gtcatcgcgg gtttacccat tcgctggtgt
     1681 ttgcgtttgt tgtcccgctg ttatgtgtgc tgattggacg ccgatggttc agggctgggc
     1741 tgattcgctg ctggctgttt ttaaccgtct cgttgctatc gcacagcttg ctggattcgg
     1801 taaccactgg cggtaagggc gttggctggc tgtggccgtg gtcagatgaa cgctttttcg
     1861 ctccctggca ggtgattaaa gtcgcgccgt ttgcattgtc tcgttacacc acgccgtacg
     1921 ggcatcaggt gataatttct gaattgatgt gggtgtggtt gccggggatg gtgcttatgg
     1981 gaatgttgtg gtggcgcagg cgataaccgg atgcgaaaac tcgcatccgg caacagcgca
     2041 attacttacg acgccaggtg gtcccttgcg ggccatcttc cagcacgatc cccatctcgt
     2101 taagacggtc acgcgccgca tctgccgccg cccagtcttt cgctttacgg gcatccagac
     2161 gctgttgaat taacgcttca atctcagcca cttcgctgtc gtctgcctgc gcgccgcttt
     2221 gcaggaacgc ttccggttct tgctccagca ggcccaatac agcggaaagt ttacgcaggt
     2281 gagatgccat tgcattcgcc gctgccatat cttctgcttt cagacggttt acttcacgcg
     2341 ccatatcaaa cagtacggaa taggcttccg gggtgttgaa atcgtcgtcc atcgcttcaa
     2401 taaagcgcgc ttcaaacgct tcgccaccgg caggcgcaac ggttttatct gtgccgcgca
     2461 gcgcagtgta gagacgctcc agcgccgcac gcgcttgctt caggttctct tcgctgtagt
     2521 tcaactggct gcgatagtgg cccgacatca ggaagtaacg cacggtttcc gcgtcgtagt
     2581 atttcagcac atcgcgcacg gtaaagaagt tacccagcga tttggacatc ttctcgcggt
     2641 caaccatcac catccccgag tgcatccagt agttcacata ctggccatca tgggcacagg
     2701 tggactgcgc gatttcgttc tcgtggtgcg ggaacatcag gtctgaaccg ccgccgtgga
     2761 tatcaaagtg gttacccagc tgcttgcagt tcattgccga acattcaatg tgccagcctg
     2821 gacggcccgc gccccacgga gacggccagc tcggttcgcc ctctttcgac atcttccaca
     2881 gaacgaagtc catcgggttg cgtttgtcgt cgaccacgtc aacgcgcgca cctgcctgca
     2941 gctggtcgag atcctgacgc gacagcacgc catatgtcgg atcggtcggg acgtcgaaca
     3001 tcacgtcgcc gttgtccgcc acataagcgt gacctttggc gatcagttgt tcagtgagtt
     3061 caataatttc tgcgatatgg tgcgtcgcgc gcggctccat atccgggcgc agaatgttca
     3121 aagcatcaaa atctttgtgc atttcggcga tcatgcgatc caccagcgcc acaaagcttt
     3181 cgccattttc attggcgcgt ttgatgattt tgtcgtcgat atcggtaatg ttgcgcacat
     3241 acttcagttt atagccgagg aaacgcagat agcgcgcaac cacgtcaaaa gcaacaaagg
     3301 tacgcccgtg accgatatga cagagatcgt aaacggtgat tccacacaca tacatgccga
     3361 cttccccggc gtgaataggc ttaaattcct ctttttggcg tgtcagagta ttgaagattt
     3421 ttagcatcga agattccgtt tagacatgtg tgggtaattg agttgcgtat aatacccata
     3481 tttcccgcgc gaatcagcat acattgcgtg atgatccgat catgcggtta tgctataaca
     3541 ccaccctata tatgacccga actgggttga agcaccaatc aaacggaaca ggatgcaaaa
     3601 atggttactt tccacaccaa tcacggcgat attgtcatca aaacttttga cgataaagca
     3661 cctgaaacag ttaaaaactt cctggactac tgccgcgaag gtttttacaa caacaccatt
     3721 ttccaccgtg ttatcaacgg ctttatgatt cagggcggcg gttttgaacc gggcatgaaa
     3781 caaaaagcca ccaaagaacc gatcaaaaac gaagccaaca acggcctgaa aaatacccgt
     3841 ggtacgctgg caatggcacg tactcaggct ccgcactctg caactgcaca gttcttcatc
     3901 aacgtggttg ataacgactt cctgaacttc tctggcgaaa gcctgcaagg ttggggctac
     3961 tgcgtgtttg ctgaagtggt tgacggcatg gacgtggtag acaaaatcaa aggtgttgca
     4021 accggtcgta gcggtatgca ccaggacgtg ccaaaagaag acgttatcat tgaaagcgtg
     4081 accgttagcg agtaatcgtg gcgacactct ttattgcaga tcttcatctc tgcgtggaag
     4141 aaccggcgat caccgccggt tttctgcgtt ttttagcggg ggaagcccgc aaggccgacg
     4201 cgctgtatat tcttggcgat ctgtttgaag catggattgg cgacgacgat cccaacccac
     4261 tccatcgcca gatggcggca gcgatcaaag cggtgtccga ttccggcgtt ccctgttatt
     4321 tcattcatgg caaccgtgat tttctgctcg gcaaacgctt tgcccgtgaa agcggcatga
     4381 cattattgcc ggaagaaaag gtgctcgaac tttatggtcg ccgggtgttg attatgcatg
     4441 gcgacacgct gtgcaccgat gacgcgggtt atcaggcttt tcgcgccaaa gtccacaaac
     4501 cctggctgca aatgctattc ctcgccctgc cgttgtttgt gcgcaaacgc attgccgcgc
     4561 gaatgcgagc gaacagtaaa gaagccaaca gcagtaaatc gctggcgatc atggacgtta
     4621 accaaaacgc ggtagtcagt gcgatggaaa aacatcaggt gcaatggctg atccacggac
     4681 atacccatcg cccggcggta catgaactta tcgccaacca gcaacctgct tttcgcgtgg
     4741 tactcggtgc ctggcatacg gaaggttcga tggtgaaagt cacggcggat gacgttgagc
     4801 tgattcattt tccgttttaa aaaacccgca actttgctga tttcacagcc acgcaaccgt
     4861 tttccttgct ctctttccgt gctattctct gtgccctcta aagccgagag ttgtgcacca
     4921 caggagtttt aagacgcatg tcttcccgca ataatccggc gcgtgtcgcc atcgtgatgg
     4981 ggtccaaaag cgactgggct accatgcagt tcgccgccga aatcttcgaa atcctgaatg
     5041 tcccgtacca cgttgaagtg gtttctgctc accgcacccc cgataaactg ttcagcttcg
     5101 ccgaaagcgc cgaagagaac ggttatcagg tgattattgc gggcgcaggt ggcgcagcgc
     5161 acctgccagg catgattgcc gccaaaacgc tggtgccggt gctgggcgtg ccagtacaga
     5221 gcgccgcact gagcggtgtc gatagcctct actccatcgt acaaatgccg cgcggcattc
     5281 cggtgggtac gctggcgatt ggtaaagctg gcgcggcaaa cgcggcatta ctggcagcac
     5341 aaattctcgc gacttacgat aaagagctac accagcgtct gaatgactgg cgcaaagccc
     5401 agaccgacga agtgctggaa aacccggacc cgcgaggtgc ggcatgaaac aggtttgcgt
     5461 cctcggtaac gggcagttag gccgtatgct gcgtcaggca ggcgaaccgt taggcattgc
     5521 tgtctggccg gtcgggctgg acgctgaacc ggcggcggtg ccttttcaac aaagcgtgat
     5581 taccgctgag atcgaacgct ggccggaaac cgcattaacc cgcgagctgg cgcgtcatcc
     5641 ggcctttgtg aaccgcgatg tgttcccgat tattgctgac cgtctgactc agaagcagct
     5701 tttcgataag ctccacctgc cgaccgcgcc gtggcagtta cttgccgatc gcagcgagtg
     5761 gcctgcggtg tttgatcgtt taggtgaact ggcgattgtt aagcgtcgca ctggtggcta
     5821 tgacggtcgc ggtcaatggc gtttacgcgc caatgaaacc gaacagttac cggcagagtg
     5881 ttacggcgaa tgtattgtcg agcagggcat taacttctct ggtgaagtgt cgctggttgg
     5941 cgcgcgcggc tttgatggca gcaccgtgtt ttatccgctg acgcataacc tgcatcagga
     6001 cggtattttg cgcaccagcg tcgcttttcc gcaggccaac gcacagcagc aggcgcaagc
     6061 cgaagagatg ctgtcggcga ttatgcagga gctgggctat gtgggcgtga tggcgatgga
     6121 gtgttttgtc accccgcaag gtctgctgat caacgaactg gctccgcgtg tgcataacag
     6181 cggtcactgg acacaaaacg gtgccagcat cagccagttt gagctgcatc tgcgggcgat
     6241 taccgatctg ccgttaccgc aaccagtagt gaataatccg tcggtgatga tcaatctgat
     6301 tggtagcgat gcgaattatg actggctgaa attgccgctg gtgcatctgc actggtacga
     6361 caaagaagtc cgtccggggc gtaaagtggg gcatctgaat ttgaccgaca gcgacacatc
     6421 gcgtctgact gcgacgctgg aagccttaat cccgctgctg ccgccagagt atgccagcgg
     6481 cgtgatttgg gcgcagagta agttcagtta agcaggtgct catttgattg ccggatgcgg
     6541 cgtaaacgcc ttatccggct taccgctccg gtacccattg taggcctgat aagacgcgtc
     6601 aagcgtcgca tcaggcatgg tgcaccgatt gccggatgct ctcatcacta cagcgaaata
     6661 caggtccccg cttcgcccgc cagcgtctct tcaattcgcg ataacgcccc aatccacgcg
     6721 ggtttactac ggcttctgac ataaccactc accgccgtta ccttcggccc catcgaacca
     6781 tcggctttgg caaatggcgc taactcatcc ggtgtggcat ggcgaatggc acgttgctgc
     6841 ggcgttcccc agttttcata taccgcatca gcatcggtga ggatcaccag tccatcggca
     6901 ttaatctgct cggcgagcaa cgcagcggcg agatctttat caatcaccgc ttcactccct
     6961 gccccatctt ccgtcacagg cacaccgccg ccgccactgc aaatcaccac atgcccctct
     7021 ttgagcaaca actcgatggc ttcgctgtcg agaattttac gcggttgcgg agacgccacc
     7081 acccggcgca agtatttacc atcacgtttc atctgccagc cgtaagctgc ttccagtgcc
     7141 tcttgttctt ctggctgata aaccggacca ataaatttct ctggctgcaa aaacgccgga
     7201 tcatcaggcg aaacctcaat gcgcgtcagc accgtcgtaa caggcggcat ctgcggctgt
     7261 gcgctcaagc tctgcgccag catatagcca atcatcccct ggctttccgc caccagcaca
     7321 tccagcggat acggttctac ctctttccac gccagattct gtaacgccag cagccccacc
     7381 tgcggcccgt tgccgtgaac aatcgccagc cgataagaac gggccaggcg tgccagcgcg
     7441 ggtacagcac tggcgatatt gcgatattga ttttccgccg tcagcgcctc accgcgctgg
     7501 agtaaggcgt tgcccccaag agctacaacc agtgttttca tggttttcct tgtaataatt
     7561 gttggccaag ccagaacccc agcagcgtat ctgcccctga cgtatgcccc agcgccagca
     7621 gcgaatcaat cgcaacagcg gtacgtttcg ggcaactcag agcatgaaca aagtgcagga
     7681 gtggcgaggc gaaatatcct tgcgcggcat aacgtaaata actgacgctc accgctgtgg
     7741 taacgagttg aagattgtcg gaacaggcaa aaaacgggcg accagagcgc gcatctaaag
     7801 cgccataata ccaggccgcc agcagcattc cgctcaacgt gtcatcatga cttggcgtta
     7861 atccgggtcc tttacccagc cagtgccgcc agtcggtctt aacgccattg agcgcggcct
     7921 gaaaacagtg acgaaactgg cgtaattcag cggtcagcgg atcgcttgcc gccaacgcca
     7981 gtggcccgaa aagcccggtt tcctccgcgc gttgcatcca tgcagctgca agcggttgag
     8041 gatgcgcagg cggcgtaata cgcagcaaac aataacgctg tggctgttta accgtgaaac
     8101 gcccgaggcg aatcccttga gccacaacct gtggtcgttc attgccgcat aatccaccgc
     8161 ataacgcatc gaattgcgcg cggcgaagca cccatcctcc ggggccgaaa ccactaccct
     8221 gacgatgcag cgttaacagt tcgccgcttt ccgtcatcag gttaatcgcc cgccgccaca
     8281 ctcccgctaa ccgccaggac tggcgataat tcggtgcact acggctggca agcagaggat
     8341 ggatgatcgt cattgcgcgt tcctcaacca atccccatgc tttcagccag tgcttccagc
     8401 gcctgttcaa aacacgccag cggtgcccgc acagtgcctg cgccaatctg cccgatcccc
     8461 gcctctttat gggcgatacc ggtattgatg agtggcgtaa tgccggtttc taccacgcga
     8521 cgaatgtcca gtcccaggca cgcgccctga aaatcccagc ctgggatctg caactgcata
     8581 ttgcgctcaa ggtaaatttc cgccatctct tcagataccg ctctggccgc ttccatgcca
     8641 cccgcaccga caaagcgcgt tacgccaggc gctgcgatca ttgccgcacc tccgatacca
     8701 aaggtttcgg taatcgcgct atcgcccata tccgggttcg cctgctcctg cgagaagccg
     8761 gtgaaaaaca gaccttgcgg agtgtttaca ggcgcagtaa accagcgttc gcccagcccg
     8821 cttacccgaa tcccgaacat attgccgttg cgggtcattg ccgtgacgat gctgcctgcg
     8881 cggatcatcg cgccagcatc catcgccgcc ttgcagtaag ccatcgcgag gttgaggaag
     8941 aactgatcgg tcacgctgag gaaatccatc acttcggcga tgtgctgttt atcatgatcg
     9001 aggcgagcaa tttgtggggc cagcgcacgc attaacagtg cagaggaagc aatattgcgt
     9061 tgatggaact catcgcccat cgtaatgccc tgcgccatca tcgccgtgag atcgataccg
     9121 cgctccatgc gccccagcgc cgcgcttaat actggcatta acacatcgcg catccagcga
     9181 tggcgagtca ggacatcttc gccgtaagcg ccaaaacgca tcactttgcc gataccttcg
     9241 ttgaggttgc agtacgcccg gttgccgtcg gtcacgttct cgaccaccag catcggcata
     9301 ctggcagaag taataccgcc cattggaccg acggcattca catggtgaca aggaatgaag
     9361 ttcacttccc cctgctccag tattgccagc gcctgcgctt catctttcgc ccagccttcg
     9421 aacagacatg cgcccacgca cgcccctttc atgggtccgg tcatctcctg ccagcgcatt
     9481 ggcggcccgg cgtgaagcag tgttttgccc tcgtttagtt cgctgataag tgaagaagcc
     9541 ggttgcacat ccagccagtg tggacgagcg cgacgaattt gttcgataac cgcagcattg
     9601 gcttgcgcca ctgatgtaaa catgggaacc ccttattgca aacgttctaa taaacgagcc
     9661 agttttttat tgccgccggc gactggcgac cattggtaat gcacaaccgg tttgctggcg
     9721 ctttgtagct ccagcgcaaa gctgcgtaat ccgatgttaa tcacggcgac gttttccagt
     9781 aatgacggtg tgtgttgctg tgtggcagac gagagcggat gaattaacgc tgccgccagc
     9841 aaggtggctt ccggtagcga actcactacc gcgatccccg catcttccag cgtggcgatt
     9901 tgctgcgagc ggcattgcgg gtcacgttcg gtacctgtca ccgtggcaat ggcgtacagc
     9961 ggttggctgt ctgaacgcgc ggcacaggct ttttgccagg cgctcaccag cgaggcagca
    10021 ggatcggcgg tcgcaccgaa gccaatcacg acatcaagca gcaacacgcg cacttgcggt
    10081 ttagcgccga gatcggcaat taactggttg cgtaaggctg ggtcgatcat cggatgggga
    10141 cgcccgacgg tgtagaaatc atcgcccaga tcaatgatct ggtgaccatc ggcgtccagc
    10201 atcatgccgt gatgatgggt atcgtcggct tccacgccaa ggtgcccggc aagtaatccc
    10261 gccgcttcgg cagcaagcgt accgccggta tacaaaccgc aaataaatcc gctgctgaca
    10321 ggcgctattg cgttacgtcg cgccgtgacg cgtgaaagca ggcaagccag gcgtgcggcc
    10381 tcatccagcg aggaggcaaa ccagacattc tcgtcgcggg ccaccgccgg ggtataacct
    10441 aaaaacagcg ccaccgtcgg tttgccggtt gctttcatgg cattaacaat tttcagacgc
    10501 acagcttcgg caggtggttt tgaaacaaat gccagcactt cgcttttctc gtctgcactg
    10561 agcatttcca gcgctgttag cgcactgatg ccgcccactt cacggctgag gtcgcgcccg
    10621 ccaaggccaa tcgcgtgagt aattccctcc cctgccagcg caatctgcga acacagctcc
    10681 tgaatcccgg taccggaagc gccaatgacg ccaatattgc cttctggcat cacgttagca
    10741 aaagccagcg gtgtgccggc aatcatcgac gtaccgcagt ccggccccat caccagcaag
    10801 cctttttccc gcgcgcgggt tttaagttgg atttcatctt ccagcgtgac gttatcggag
    10861 aacatcatca cgttgaggtt gcgatccagc gcctggtttg tcagctccgc cgcatactcg
    10921 ccagccactg aaatcagcgc cagattggca tcgggtaatt tctgacaggc gctgtcccag
    10981 cgacgcacct gcgtcaacgc ctggctgctg cctgacccct gcgccagttg ttttagcgcc
    11041 tcttcaagct gctgcataat cgcctgcgcg atccccgcat ccgccgcttc gctacgaatt
    11101 gccacgcaaa tatcgttcgg cgtggcgtta ttaaaatcgt catgccagaa acctgtggta
    11161 tctaataacg ctttattggc gggcgtaccc atcattacgg aaacatcatc aacattttct
    11221 gattcgctga gttttcgtga aataatcatt aaactgaccg aatcctgaaa acaccctttt
    11281 ttaataaagg cgtggatcat aacaactcct taaaaattgc acccatggca attgcacgag
    11341 cagtgttaat gtggcgatta cggtatcgat gacaacgagt aaataaggtg atgaatatca
    11401 cacagggatt attaacaaaa gaattcaaat aaccgccagg ctattacgca ttatttttta
    11461 ataagcgtgg ttgttttaaa aatcaggtta aaagcgcgaa gattatcaaa taaattctaa
    11521 ctatattttt ttgcctgtct ggatcacata atccagatat tttccctgta tgttaattgc
    11581 agtcatgctt cacaccgtcg ttaaaaagga agacagatga aaatcagtcg ggaaacactc
    11641 caccagctaa ttgagaataa actctgccag gctgggttaa aacgtgagca cgctgcaacc
    11701 gtggctgaag tattggttta cgccgatgcc agagggatcc actctcatgg cgcggtgcgc
    11761 gtggaatact acgcggaacg catttcaaaa ggcggcacca accgcgaacc ggagtttcgt
    11821 cttgaggaaa ccgggccgtg ctcggcaatt ttacatgccg acaatgccgc cggacaggtc
    11881 gcggcgaaaa tgggtatgga acatgccatc aaaaccgccc agcaaaatgg cgttgcggtg
    11941 gtcggtatca gccggatggg tcacagcggc gcaatctctt attttgtgca gcaggcagcc
    12001 cgcgccggat taattggtat ttcgatgtgc cagtccgatc caatggtggt gccgtttggc
    12061 ggcgcggaaa tttactacgg tactaaccca ctggcctttg ccgcgccggg agaaggcgac
    12121 gagatcctta cctttgatat ggcgactacc gtacaggcat ggggaaaagt gctccacgcc
    12181 cgctcgcgta atatgtctat cccggatacc tgggcggtcg ataaaaacgg tgcaccaaca
    12241 accgatccgt tcgcggtaca tgctctgctc cccgccgccg ggccaaaagg gtatggcctg
    12301 atgatgatga ttgacgtcct ctcaggcgtc ttactcggct taccgttcgg gcgacaggtt
    12361 agttcgatgt atgacgattt acacgccggg cgtaatttgg ggcaattaca tatcgttatt
    12421 aacccgaact ttttcttctc cagcaaatta tttcgtcaac atcttagcca gaccatgcgc
    12481 gaattaaatg ccattacccc cgcgcccggt tttaatcagg tttattatcc cggacaggat
    12541 caggatatta aacaacgcaa agccgccgtc gaaggcatcg aaattgttga tgatatttac
    12601 cagtatttaa tttccgacgc gctttataac acgtcatacg aaacgaaaaa tccctttgcg
    12661 caataattat tgagacagga ctttcttatg attacacatt ttcgtcaagc catagaagaa
    12721 acgctgccct ggctttcctc ttttggcgct gacccaacgg gtggtatgac ccgtttactt
    12781 tattcgccgg aatggctgga aactcagcag caatttaaaa aaagaatggc agcaagcggg
    12841 ctggaaacac gtttcgatga agtggggaat ttatatggtc gcctgagtgg caccgaatat
    12901 ccacaagaag tggttctgag cggttcgcat atcgataccg tggttaacgg cggtaacctc
    12961 gacgggcaat tcggcgcgct ggcggcgtgg ctggcaattg actggctgaa aacgcaatac
    13021 ggcgcgccgc tacgtacggt cgaagtggtg gcgatggcag aagaagaagg cagccgcttc
    13081 ccgtatgtct tctggggcag taaaaatatc tttgggctgg cgaatcctga cgacgtgcgg
    13141 aatatctgtg atgccaaagg aaatagtttt gtcgatgcga tgaaggcttg cggatttact
    13201 cttccgaacg ccccactaac tccgcgtcag gatattaaag cctttgttga actgcatatt
    13261 gaacagggct gtgtgctgga aagtaatggg caatcaattg gcgtggtgaa tgcaattgtc
    13321 gggcagcgtc gttatacggt aacgctgaac ggcgaatcaa accatgcagg caccacgccg
    13381 atgggttatc gtcgtgatac agtttacgct ttcagtcgca tttgccatca gtcggtcgaa
    13441 aaagcgaaaa ggatgggcga tccgctggtt ctgacctttg gcaaagtaga gccgcgcccg
    13501 aatacggtaa atgtggtgcc gggtaaaacc acgttcacca ttgattgtcg tcataccgac
    13561 gctgccgtgc tgcgcgattt cacccaacag ttagaaaacg acatgcgggc gatttgcgat
    13621 gaaatggaca ttggtattga tatcgattta tggatggacg aagaacccgt gccgatgaat
    13681 aaggagctgg tcgccaccct gacagaattg tgtgaaagag aaaaactgaa ttaccgggtg
    13741 atgcacagtg gtgccgggca cgacgcgcaa attttcgcgc ctcgcgtacc aacctgcatg
    13801 attttcattc ccagcatcaa cgggatcagc cataacccgg cggaacgcac caatattacc
    13861 gaccttgccg aaggggtcaa aacgttggca ctcatgcttt atcaacttgc ctggcagaaa
    13921 taaggagtca taaatgggat atttaaataa cgtcaccggt taccgcgaag atttactggc
    13981 taaccgtgcg attgttaaac acggtaattt cgcactgtta accccggatg gtctggtaaa
    14041 aaatattatt ccgggctttg aaaattgtga cgcgacaatc ctctccacgc caaagctggg
    14101 tgcctctttt gttgattatc tggtcacact gcatcaaaac ggtggcaacc aacagggctt
    14161 cggtggcgaa ggcattgaaa cgttcctgta tgtgatctct ggaaatatca ctgccaaagc
    14221 cgaaggcaaa acatttgcct taagcgaagg tggctatctt tattgcccac caggctcctt
    14281 aatgacgttt gttaacgccc aggccgaaga cagccaaatc tttttatata agcgtcgcta
    14341 tgttccggta gaaggccatg caccgtggct ggtttctggc aatgccagcg aactggaacg
    14401 cattcactat gaaggtatgg atgatgtgat cctgctggat tttctgccca aagagttagg
    14461 ttttgatatg aacatgcata tcctctcttt tgcaccaggt gccagccacg gttatatcga
    14521 aacacatgtt caggaacacg gtgcctatat tctttccggt cagggggttt ataacctcga
    14581 caataactgg atcccggtga aaaaaggcga ttacatcttt atgggcgctt attctttaca
    14641 ggctggttat ggtgtagggc gtggtgaagc gttcagctat atttactcga aagattgcaa
    14701 ccgcgacgta gagatttaag tcataccact cgcctgatta tttttagcta tgttggattt
    14761 tgccacggtt tataccgtgg ctttttttgt aacattgcct gatgcgcttc gcttatcagg
    14821 cctacatgat ctctgcaata tatagaattt gcgcgatttt caggaaggat atagtactca
    14881 tgccgcatcc ggcatgagta ctgcactctt atgcgatata aatcgcatcc gctttaaagg
    14941 taagggtcag tttttaattc cctgacctat tttaatggcg caggcaatat ttcgtgcgct
    15001 gttgaagaga ttggtttcac cgctggcgag tacttcggct aaaggtgcca gacgtggcaa
    15061 aatgctgaat accgcatcaa tgccgtactg gtgcaccact tccacgccat cacccaatac
    15121 gccagcaatc ccaatcaccg gtacattaaa ctgcttcgcc accgacgcca cacccagcgg
    15181 cgctttaccg cctgccgttt gcgagtcgat gcgcccttcc ccggtgatca ccagtgctgc
    15241 accctgcact gcctgcgcaa gattgaccgc attcaacaca atctcaatac ccggtttaat
    15301 atccgcattg agaaataccg ccgccgcaat acccataccg cccgcagcgc cgccgcccgc
    15361 catctggcag acattaattt ccgtttgctg ttgtaaaaca cgggcgtaat tttgcagccc
    15421 ctgttccagt tcttcgacca tttccggcgt tgcccctttt tgcgggccaa acaccgccgc
    15481 cgcgccgcgt gcccctacca gcgggttatc aacgtcacat gctacttcaa tatggcaatt
    15541 cgccagacgc ggatcgcatt catccatctc aatgcttgcc acgcgcgcta aattaccgcc
    15601 gttagccacc agcacctgac cgtcggcatc aaggaaacgc acaccgagcg cctgcgccat
    15661 acccatacca ccgtcgaccg tcgcactgcc gccaatgccg agaataatat ggcgaatgcc
    15721 gttatccagc gcatgacgaa ttaactcccc cgtaccaaaa ctggaggcca gcaacggatt
    15781 acgcttttca ggcgcgacca gcatcaggcc acttgctgcc gccatctcaa tcaccgccgt
    15841 tttcccgtcg ccggtaaggc cataaaaagc attcactttt tcgcccatcg gcccgcagac
    15901 ttcaagcgtc acgatgttgc cgcccgtcgc ggcgaccatc gcatccaccg ttccttcgcc
    15961 gccatccgct atcggcaaac agatatagtt cgcatcggga aagagggtcg aaaacccggc
    16021 tttaattgcc tgacaacatt tttctgcact taagctctct ttaaaagagt ctggcgcaat
    16081 gacaatcttc attatcaatc ctttttcaac aaattattcg atacgttccc acggaataag
    16141 gttatccata agaacagcca gtaaaatccc gaccaataag ccgttactga gcagaggacg
    16201 aagcgttaat ggaaggtctt gcagatacac aggcggtaat gccattaaaa atatgccgac
    16261 aaataacggc aatgcgagtc gataaatatt gcgagcggta aaggttattt gctggctaaa
    16321 caccagcgcg gaaaagagta aaggcagata agaaaccagc atgaccgcgc tactcacggg
    16381 taaagggata ctgcaaaaca gtcgtgtgag tgccggaacc agcgccacca gcaggcaaat
    16441 aacgctgcca taaataaacg aacgccgcgt gtaatcgcct gtctgggtta ataaaccaat
    16501 ggatgaaaca aacggtgaaa atggaattac tgccagcggt acggttagca gtgtcataaa
    16561 tccggtcgcc acaaagctac gacgataacg cgtattcccc gcgccctgct gcggataaaa
    16621 aacatccgtg ccccgaatcg caccgtaggt attgctgata ttcaccagac ctgtaatcac
    16681 cgccgtcaga ataattcccg gagacaaagc acctccactg ccgagcggga accactgcca
    16741 gtgcaactca ccggagagcg agtgcgaaga aggaaagcaa aagtaccaca acaaccagcc
    16801 ggttatggtg ccgaccagca ggccataacg ggcaaaacgt tgcggcagga agataatcat
    16861 ccccagtacc aggcacatca ccgccaccga gagcgcgaac ggcggtaact gaattttaaa
    16921 atttgggtcg gctatgccaa acggcagccc gagcatacct ttgaaaaaga tagtggtcag
    16981 ctgcgcgccc agcatcaaca taaacaagac catcaccgac ggcgtaaata accgtgccag
    17041 gcgatggcct aatccgctaa aaccaatcaa catcgtcagc acgccggaga gcgcaatccc
    17101 caccgccagg ctggtagcga tatcgttgat cggtgtcccg cgcgatgctt cgccaagggt
    17161 gatagtaagg atggttcccc accacaggcc acccggccct tccataatag cgcgacgatg
    17221 accgcagaac gcctgagcga agcaggccag tgcggtagca agaaaagcat attgcgtgag
    17281 cgtaagcagg ctactttgcg gcaactgaaa agcagaaagt agcgtaggag gaaccacaac
    17341 cgtgttgcaa aaaataaaga aaaaccactg aaatcctgat aacaggcttt cgcggctcac
    17401 tgcaaaattg aacataaaca caccttaacc ggagaatgcc cgccgcaagg acgggcattg
    17461 caggggccag attactgctg atgtttaagg ataaattgac ctttcggcgc aacagggaag
    17521 ccctgttcaa tgtcgtaaat cacatcacca cgtaagatgg ttttcgtgat acgcgcgcca
    17581 atggtacggc caacatacgg gctgactttg tggcgatatt ccagatcgtc attggtaaga
    17641 acatagctgc tattcggctg aatgaagacg aagtcggcat cttttcctgg ggcgatacgg
    17701 cctttttgct gcagaccgaa aatatctgct gcgttagtcg ccattaattt gccgaacatt
    17761 ggcagagaca ttccgcgttt ctgtaccgct tcatcgaaca tcacgtccat acagttttgc
    17821 agaccggcaa taccgcccca tgcttccatg atgttgccgg ctttcatttc cggagggcat
    17881 ggtgagtggt cggaaaccag gcagtcgatt tcaccgataa acagtttttc ccacatgcct
    17941 ttctggtttt ccagatcgcg gatcggcggt gaacacttcg ccagagtgcc gatttcttcg
    18001 aactgatcgg tatccagtac aaagtaatgc gggcaggatt cacaagtaac gtcctgacct
    18061 tcctgacgtg cacgagtcac ttcttcaacg ccttccgggc tgctgatatg gcaaacgtgc
    18121 agacggcaac cggcaacttt cgccagatac agcacgcggc gaatcgcttc cacttcggta
    18181 aataccggac gcgaagccac atagtcatgg gcagttacgc gaccttcacg tttcgcttct
    18241 tcgcccagtg cgtcacagat cagcgcgttt tcgcagtgca ccagcaccgg ctgccccagt
    18301 tcgcccagct tctgcgcacc tttgaagaac tgccagtcgt ttacatcacg gaagtcgttg
    18361 tcgataccgc gatcgccaca ggtcgcaacg aagcatttga agccgacaac gcccacttca
    18421 tccagctcat gcagacggtc gatgttgtaa gacaccaggc caccgagttg tgccgcatcg
    18481 atagtcagct tgcctttagc ggcatcgaac ttcagttcaa ttgaagcgcg gtcaaccgtt
    18541 gcaggcagct ggttgagcgg catttcgatc atggtggtga taccaccttt tgccgctgcg
    18601 cgagtaccgg tttcataacc ttcccagtgg ctacgacccg gttcagaaat atgggtgtgc
    18661 gcatcaacca tgcccggcga aaccaccaga ccagacgcat ccataacttc ttttgcatcg
    18721 cccagatcct gaccgatagc agcaattttc ccgcctttaa cggcgatatc tacaacgcga
    18781 gcttcgtttt ctaaaataac ggtgccgttt ttaatgatta aatcaaaaga cataacaaac
    18841 tccttgtaaa atacagattg gtttttaact caatgttcaa tatcgggatt aattaaccta
    18901 tggttttttg ctctcctgtt ttttctgctg ttgtacgctt ctttaataag gcgtaggccg
    18961 caaaggcgac gatgacgccg acaaaccatg aaacacgcga taacggttcc ataaagggaa
    19021 taaacttacc gccaagagat aaaataacgg ccaccagagt tactgaaaac gcagtgaggt
    19081 taaaaccgtt atcgtaatat ttataatcgc caggtgcggt atacagttca tcaagattaa
    19141 ttttcccacg catcaccaca aaataatgcg ccatcatgac accaattacc ggaccaagca
    19201 ttccgccgat aatatcgagg aacagataaa tactgtcctg attttccatt aatttccacg
    19261 ggcagatcag caggctgata atactggcaa tcagtacgcc gtttttatag ttcagtttcg
    19321 tcggtgcaat agcggcaatc tgataaccgg caggaataat attaccggtg gcgttagtgg
    19381 agatagtcgt catcagaata accagtaccg caaagaacga ggcgaacaag ctgtcccaac
    19441 gctgaacaat atccagcacg ttccaggtat cagcgccgta atgaatactg gctccggcaa
    19501 taatacatac ccccgcgacc gcaaacagaa tataggccac aactaaaccc agcgtttgcc
    19561 ccagcgcttg ctcacgaaac gagtgggcgt tttgcgtaaa gtcggatgcg ctcaccgccg
    19621 gtgccgccca gaccgcaact accgcgttta tcaccaccag gaacaggaag ccactgtttt
    19681 ctgctttctg aataccgctc ggaatgtagt caaagattgg accgagcccg accagcgaaa
    19741 tcgcccaaat cgccataccg ccgaaaacga tatagatgca cgggttaaga atggcagtga
    19801 atttatttaa aactttgcca ccgccaaaac ctataccaac gttgaccagc cagaagagta
    19861 agaaagtaat taagcccggt agagaaagcc ctaacagcgt gaaatcacca ccgagagtta
    19921 aaaatcccgg ccagattttg ccaatcagaa tcaagcaggc cagtgacccc gcgtaacatt
    19981 gcaggccaaa ccacataatg gcagcaatac cgcctcttaa taatccggga aacagtgcgc
    20041 cacgtacgcc gtaagaagca cgcaggatca tggcaaacgg tacgccgtat ttactgcccg
    20101 cagcgccgtt taataccatt accgcggcaa tgaaaaaggc gctgaggata attgccagca
    20161 taatactaaa ggtagacaag ccgagaataa aaaagccgcc gaccatcaca taattgggaa
    20221 cgttatgaac cgaacccatc cataaggtaa aatagttaaa tgttttccag gtccgctggc
    20281 tttgcgtttt cggcaataga tcttcgctat agccgcgttg ctggaatagt tttctctgat
    20341 gttccataaa tccctcttaa tttcacgtat cgaataacgt tgttttaatc tagctatgag
    20401 gaacaaataa aataaaaata ttatttttaa tactgaggca acatctttgg ctgctttctt
    20461 ttttaatatt gctggccgat tcattttctg atatcggcca tttttattgc gggtatcagg
    20521 ccagtttatg gttagccatt aattccagcg cctgcactaa cgcagagtga tccaactggc
    20581 tgccaccgtt tgccgcacag gtattaaata actcctggca ggtcgcagtg tttggcaggt
    20641 tcagcgcaag tgctttcgca ctttgcagtg ccaggttgag atctttctgg tgcagagcga
    20701 ttttgaagcc cggattaaag gtgcgtttaa tcatacgctc gccatgaact tccagaatac
    20761 gtgaggaagc aaagccgccc atcagcgcct ggcgcacacg taccgggtcc gcaccggctt
    20821 ttgaagcaaa tagcagggct tcagaaaccg cttcaatatt gagcgccacg ataatctgat
    20881 ttgccacttt gcaggtttga ccgtcgccgt taccgcccac gagggtgata tttttaccga
    20941 gcagttcaaa cagcggttta acacgttcaa ataccgcttc atcaccgcca accataatcg
    21001 acaacgtccc ttcacgcgca ccgatttcac cgccggagac tggcgcatcg agataatcgc
    21061 cgcccagttc attcacctga cgagcgaaac gcttagtttc aatcggggaa atggagctca
    21121 tatcagcaat ggttttgccc ttcagcgagg ctttggtaca accattttca ccgaacagaa
    21181 cttcttcaac ctgtggtgta tctggcacca taataaaaat gatgtccgat gcttccgtta
    21241 cctggcgcgc agtttcaaca ctgacggcac ccagtgacag taattcatca gcaaccggac
    21301 caatggttgt gacatgcagt tgatgaccgg cacgcgccag attaatggcc atcggtgtac
    21361 ccataatgcc taagccaata aatcccagtt tcataattaa cctcttttaa atttcgcttt
    21421 tcctgaattc agacaacacg atcccctgcc aacttagcgg cctaaaaaag cattgcctga
    21481 acagcgttac gttttaacgg tacggatcca tccagcgtaa accggcttcc gtggtggttt
    21541 ggggtttata ttcacaccca acccaaccgt tgtagtcaga attttcgatt accttaaaga
    21601 gataatcata attaatttct ccggtccccg gttcgccgcg atgcggatta tcagcaattt
    21661 gcaggtggcc aattttatcg gcccactgag tcatggtgtt ggttaattca ccttccatcc
    21721 gctgcatgtg ataaatgtca tactgaattt tcaaattgca gcaaccaaca tcatcaatca
    21781 atttcagcgc ctgccgagtt ccggtgagat ggaaaccagg aatatcaaaa tggttaatag
    21841 gttcaatcag taataaaata tcttctttca tcagcatatt cgcggcataa cgtaggtttt
    21901 ctacaagcgt tgcgtgaatc tgttcactgc tgaaaccagc cggcgtttta ccgaccagac
    21961 agttaatttt tttattcccc agcgcacggg cataacgaat cgctgctgct acgccatccc
    22021 gaaactcttc ttcacggcca ggaatacagg caataccgcg ctcccccgcc gcccagtcac
    22081 ccgccggtag attgtgcagc gtatgttcga gtttattact cgccagcacc tgttttaatt
    22141 cttcaatgtc gtagtcataa ggaaacataa attcaacgcc gcgaaaacca cactgcgcag
    22201 ctttctcaaa acgggcgaga aaatcatatt ctccaaataa catcgataaa ttagcagaga
    22261 aacgtaacat tatttatctc ccttattcat agtgcatgaa gcaggtttca gtcggtgcgt
    22321 ccgctgcgtt atcggcgata tcttcaaatt ccatgacgtt atgcagttcg ctgcccatcg
    22381 aaatattggt cacacgctcg agaataactt ccacgactac cggtacccga tattgcgcca
    22441 ttaaggcttt cgcctgttca aaggctggcg caatatcttc cggtttgaag acgcgaatcg
    22501 ctttacaacc taaaccttcc gctactttta cgtggtcgac gccgtaaccg ttcacttcgc
    22561 tggagttgat attctcgaaa gcgagttgca cgcagtagtc catatcaaac gcgcgctgcg
    22621 actgacgaat cagccccaga taagcgttgt tgaccagcac atggatgtac ggaatgttga
    22681 actgcgcgcc aacagctaac tcttcaatca ggaactggaa gtcaaagtcg ccagaaatcg
    22741 ccaccacatt gcgtttcgga tcagcggcac aaacccctag cgcagccgga atcgtccagc
    22801 ctaacggacc agcctgacca cagttgatcc agtggcggtc tttaaagaca tgcagcattt
    22861 gcgcagcggc aatttgcgac agaccaatgg tggtgacata gcaaacatcg cgaccaaagg
    22921 ctttgttcat ctcttcatac acgcgctgcg gtttcaccgg cacgttgtcg aaatgggttt
    22981 tgcgcagcaa agtgcgtttg cgctgctggc agtcggcgac ccattcttta cgacacggca
    23041 gacgacccgc tttttgcatc tcctgcgcca cttcaaccag cagtgtcagc gccactttag
    23101 catcagagac aatgccgaga tccggacaca gcacgcgacc aatttgcgtc ggctcaatat
    23161 cgatatgaac gattttgcgc ccttcggtgt atttctctac cgaaccggta tgacggttag
    23221 caaaacggtt accgatacca aacaccatgt cggacgccag caacgttgcg ttaccgtaac
    23281 gatgcgcggt ttgcagaccc accatcccgg ccatcagttc atgatcgtcc gggatacagc
    23341 cccagcccat cagcgttggg atcaccggaa cgctggtcag ttcagcaaac tgttgtaaca
    23401 gtgcagctgc gtcagcatta attaccccgc ccccggcaac aatcaccgga cgttcggcct
    23461 ggattaacat ttctacagct ttttcgatct gcatacggct ggcagcaggt ttgtagaccg
    23521 gcagcggttc gtacatgtca ggatcaaact cgatttccgc aacctgaacg tcgaacggta
    23581 aatccaccag taccggaccc ggacgaccag aacgcatcag gtgaaatgcc tgttgcagca
    23641 cgcgaggcac cagcgccgct tcacgaactg taaccgccat tttgctgacc ggtttagcaa
    23701 ttgcttcaat atctacggcc tgaaaatctt ctttatgcag acgggcgcgc ggtgcctggc
    23761 cggtaatgca cagaatagga atggaatcag cagaagcgga atagagcgcg gtgatcatgt
    23821 ccgtgcccgc aggaccggaa gtccccagac atacgccgat atttcctgcc gttgcgcggg
    23881 tataaccttc cgccatgtgc gaagcacctt ccacatgacg cgccagaatg tgacgaatac
    23941 cgccgtgctt acgcatcgct gagtagaacg gattgattgc agctcccgga acaccgaagg
    24001 cggtggtgat accttctttc tccagcacat acattgccgc gtcaacggct ctcatttttg
    24061 ccattttatt cctaccttta tttattggaa aatttttcca actttcaaat tttgtcaaaa
    24121 aaacccgctc cgcagagcgg gatgcgacag acattatgga tgtgctttca gtcccaacgc
    24181 cgtgctgata tcgcgggcgg tgtctctgac cagctcaccc tgactgacaa aacgatcttc
    24241 tgtcagtctt gatgaaggcc cggagatgga gatagcggca acaacactac ctacatcatc
    24301 gtaaattgct gaagctaagc aattcagacc tacaacatgc tcttctttat ctacggtata
    24361 gcccagttca cgcgcttgtt ccaggtcctt cagcaaggtg ggcatatcca caagcgtagt
    24421 tggcgtaaac tgctgcaaac cggtttgcag aatgatgctc atcaactcct cttccgccag
    24481 cggataaagc agcgctttgc ccgcaccgga agcatgcagc ggcagacgac tgcccagtgg
    24541 cgcacacatc ctgaccatcg atttacactc taactgacca attaataccg cttcattgcc
    24601 gttacggatc gcgacattga ccgtttcgcc ggaaagtaac atcaggcggc gcataaacgg
    24661 cccggcgacg gagaggacat cgcggttatg gatgtacgct gcaccgacgt taaagacacc
    24721 taatcctata tgccaccagc ctaattgact gtcctgatag acaaaatccg ctgcctgtaa
    24781 aaccttcagc aagcgaaagg tcgtggagag cggcaaatcc agattgagag aaatatcgct
    24841 aaccgacgaa cttcccccac ttttttccaa atattgcaga atcgcaattc cccgctctaa
    24901 cgcctgtgcg cccttctgtg ccacaggctc cgcctgtcct ggcctgccgc gccgtctaac
    24961 ttccgtcata cctacgttaa ctccgaccta caagtgagca taaacaatga acaattagtt
    25021 tatcttatgc aagtcggttg caggcgtcac gcaaaacaga gttcctgttc aggaatactt
    25081 tcaacatcac agttgtcact gccgccgcga tcgatggtca gaaaatcggt gacgcgctgc
    25141 caggcgaaaa gtgggtgatg ccagacgtta cgatggtaat tcactccctg ttcgccgttg
    25201 gtgataaacg cccgcagcgt tgacaggtct ggtttgtcgt cgcctaacgc cacgaccacc
    25261 acaaacactt cacctttcat cgggataaag gcctgagtgc ccagcggatg acgttcgagt
    25321 tcgtgaatgg tcagcggcag attcgccggt tgcgcgcggt taatgctgat aagcgtacgg
    25381 tcttgctcac gaatctcaac cagcgccaaa tcgtggtaac gctccaccag gccattgtta
    25441 atatggaaaa aatcccgttg ctgcgtttcg attacgtcgc cataagcact aaaggcttcc
    25501 tgacttaacg gtaatacctg aagtttcatc tttttgtcct tgtaacataa ttccgttcaa
    25561 ccagcgccga ccggtcagct tggtttcaat aacaggcaat ctaaaacctt aagagaacaa
    25621 catcaataca aaagttggaa tatttttcca aaactagatt tagtccacaa attgaacagg
    25681 gttatcatgt aaaaatactg ataaatcaga ggataagaaa atcacgcaac ttgcggattt
    25741 aaaaggggat acctgaagat gttcgatcca gaaaccttgc ggactttcat tgcggttgct
    25801 gaaacaggaa gtttttcaaa agcggcagaa cgattatgta aaaccacggc gacgatcagt
    25861 tatcgcatta aacttctgga agagaatacc ggagtagcgc tgtttttccg tacgactcgt
    25921 agcgtaacgt tgacagcggc tggcgagcat ctactttccc aggccagaga ctggctgagc
    25981 tggctggaaa gtatgccaag cgagctgcaa caggtgaatg atggcgtgga acgccaggtg
    26041 aatattgtca tcaacaacct gctctacaac ccccaggccg tcgcccagtt gctggcgtgg
    26101 ctgaatgaac gttacccctt tacccagttt cacatctccc gacaaatcta tatgggcgtc
    26161 tgggattcgc tattgtacga aggtttttca ctggctatcg gcgtcacggg aaccgaggcg
    26221 ctggcaaata cctttagtct tgatccctta ggatcggtgc aatggcgctt tgtcatggcg
    26281 gcggatcatc cgctggcgaa cgttgaagag ccgctaacag aagcgcagtt gcggcgcttt
    26341 ccggcggtca atattgaaga cagcgcccgc accttaacca aacgcgtcgc ctggcgattg
    26401 ccagggcaaa aagagattat tgttcctgat atggaaacga aaatcgccgc ccatcttgcg
    26461 ggcgttggca ttggtttttt gccaaaatcg ctttgccagt caatgatcga taatcaacaa
    26521 ctggttagcc gggtaatccc aacgatgcgc cctccttcgc cattgagtct ggcatggcgc
    26581 aaatttggca gcggcaaagc ggtagaagat attgtgacct tgtttaccca gcgcaggccg
    26641 gaaatcagcg gatttttaga aattttcggc aacccacgca gttaaaaatc aagtttcctt
    26701 gcgcgttggc gcgtaaaata gcgcgcaagc tgatttgagg ttcactttta actatgcaag
    26761 agagacacac ggaacaggac tatcgtgccc tgctgattgc tgatacgccc attattgatg
    26821 ttcgcgcccc tatcgagttt gagcaaggcg caatgcccgc cgctatcaat ctgccgttaa
    26881 tgaataacga tgaacgcgcc gccgttggca cctgctataa acagcaaggc tcagacgcag
    26941 cgctggcgct ggggcataaa ctggtggcgg gtgaaattcg tcagcagcgc atggacgcct
    27001 ggcgggcagc gtgcctgcaa aatccgcaag gtattctctg ctgcgcccgt ggcggtcagc
    27061 gctcacatat tgtgcaaagc tggttgtatg cagcggggat tgattatccg ctggtggaag
    27121 gcggttataa ggcactgcgc cagaccgcga ttcaggcgac tattgaactg gcacaaaaac
    27181 cgatagtgct gattggcggt tgtactggca gcggtaaaac gctgttagtg cagcaacagc
    27241 cgaacggtgt tgatctggaa gggctggcgc gccatcgcgg ttcggcgttt ggtcgcacgt
    27301 tacaaccaca acttagccag gcgagttttg aaaacctgct ggctgccgaa atgctgaaaa
    27361 ccgacgcccg tcaggatttg cgcctgtggg tgctggaaga cgaaagccgg atgatcggtt
    27421 cgaatcacct gccggaatgt ctgcgcgagc gaatgactca ggcggcgatt gcggtggtag
    27481 aagatccgtt tgagatccgt cttgaacgcc tgaacgaaga gtatttcttg cgtatgcatc
    27541 atgattttac ccacgcttat ggcgacgaac agggctggca ggaatattgc gaatacctgc
    27601 atcacggact ttcggcgatt aagcgtcggc tggggttaca gcgctataac gaactcgctg
    27661 cccagctgga tacggcgctg acaacgcaac tcaccaccgg cagcaccgac ggtcatctgg
    27721 cctggctggc gccgttactt aaagaatatt acgacccaat gtatcgctat cagcttgaga
    27781 aaaaagcgga aaaagttgtc tttcgcggtg agtgggcgga agtggcggaa tgggttaagg
    27841 cgcggtaatg agggacgtat ttgtattagt cgcgacttga tctgacacat ggccttgaaa
    27901 ggttgagagt taccggtttt gatatgagtg tcgaatcctt atccaaaaca agaggtaact
    27961 ctcatgcttc atactgccaa tcccgtcatc aaacacaaag ccggtttgct caatctggct
    28021 gaagaactca gcaacgtatc aaaagcctgt aaaatcatgg gcgtttcgcg tgatacgttt
    28081 tatcgttacc gcgaactggt cgctgaaggc ggtgtggatg cgcagattaa tcgtagtcgc
    28141 cgcgctccta accttaagaa ccgtaccgat gaggcaactg aacaggctgt tgttgattac
    28201 gccgtcgctt tcccgacaca tggtcagcac cgggccagca acgagctgcg taaacagggg
    28261 gtttttatct ccgacagtgg tgtccgttct gtctggttac tccataacct tgagaatctc
    28321 aaaagacgat actgaacaaa ttttatcagg tgacgttccg taaaaagttg tatggagttc
    28381 tcgatgcagt cggcgcgact atcagtaccc ccgctgaacc ctggactatc gggccgccgt
    28441 actggggcat gtcaccctgg cgtgccgctg gttttccgct aatggtttat ttcctctttc
    28501 tttcccggca agaatttatt ttaatccgga aggattattt attttcagat agggactcac
    28561 aataaacacg taacatttta attaatgcat ggctgaatat gtattacaca atgtcagata
    28621 tcgtaaaaga ccagtgcaat tctatgtaaa ctctctgact gttcatttta tttgttgttt
    28681 cagggtcgga actcaccctc aataaacaac tgaatacgag tagatattca tcgtctgagc
    28741 tatatggctt tacacaatag ccgacatgtc ttttctcagt caggaatttt acttttcctc
    28801 actacgaagt tgacgacttc gatatgggat agactcttaa ttcaagcaat tactctgcac
    28861 aagcatcagg caatccagct ccaatatttg ttataaaata atgcgtaaca ttaatttcac
    28921 caacaaaaaa aaggtttaca ccatcccctt gccctggtat cttaccatat ttacaccaca
    28981 ctttttttgt atctggatca ttctttgata cgattttcgc cgcttgttcc ctggccctca
    29041 ctgtcaatgt tttcttaaac ctacccccat catcatttgt tagggaaaaa taagaaaaaa
    29101 aataatcatc ttctacattt gtgtatattg atatatccac cgctacgtgc ggaaaaaaat
    29161 ctgggagcca tgtaaaaact ccatcatttt ctggtattgc cata
//
LOCUS       DAZGSP010000063        29045 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZGSP010000063 DAZGSP010000000
VERSION     DAZGSP010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.063"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            63..1016
                     /gene="ybhD"
                     /locus_tag="ACHK74_003007"
     CDS             63..1016
                     /gene="ybhD"
                     /locus_tag="ACHK74_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0221782.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIS"
     gene            complement(1057..2052)
                     /gene="pgl"
                     /locus_tag="ACHK74_003008"
     CDS             complement(1057..2052)
                     /gene="pgl"
                     /locus_tag="ACHK74_003008"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFP0221783.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDDRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2207..3025
                     /gene="ybhA"
                     /locus_tag="ACHK74_003009"
     CDS             2207..3025
                     /gene="ybhA"
                     /locus_tag="ACHK74_003009"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFP0221784.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(3026..4084)
                     /gene="modC"
                     /locus_tag="ACHK74_003010"
     CDS             complement(3026..4084)
                     /gene="modC"
                     /locus_tag="ACHK74_003010"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFP0221785.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4087..4776)
                     /gene="modB"
                     /locus_tag="ACHK74_003011"
     CDS             complement(4087..4776)
                     /gene="modB"
                     /locus_tag="ACHK74_003011"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFP0221786.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4776..5549)
                     /gene="modA"
                     /locus_tag="ACHK74_003012"
     CDS             complement(4776..5549)
                     /gene="modA"
                     /locus_tag="ACHK74_003012"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0221787.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFIIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5716..5865)
                     /gene="acrZ"
                     /locus_tag="ACHK74_003013"
     CDS             complement(5716..5865)
                     /gene="acrZ"
                     /locus_tag="ACHK74_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFP0221788.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            5994..6782
                     /gene="modE"
                     /locus_tag="ACHK74_003014"
     CDS             5994..6782
                     /gene="modE"
                     /locus_tag="ACHK74_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFP0221789.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6850..8322
                     /gene="modF"
                     /locus_tag="ACHK74_003015"
     CDS             6850..8322
                     /gene="modF"
                     /locus_tag="ACHK74_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFP0221790.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRLFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPTNEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADVPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGDLYRYALTKIN"
     gene            8583..9599
                     /gene="galE"
                     /locus_tag="ACHK74_003016"
     CDS             8583..9599
                     /gene="galE"
                     /locus_tag="ACHK74_003016"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP0221791.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9609..10655
                     /gene="galT"
                     /locus_tag="ACHK74_003017"
     CDS             9609..10655
                     /gene="galT"
                     /locus_tag="ACHK74_003017"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFP0221792.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQVWANSFLPNEAEREDRLQKEYFAGQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITVLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10659..11807
                     /gene="galK"
                     /locus_tag="ACHK74_003018"
     CDS             10659..11807
                     /gene="galK"
                     /locus_tag="ACHK74_003018"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFP0221793.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11801..12841
                     /gene="galM"
                     /locus_tag="ACHK74_003019"
     CDS             11801..12841
                     /gene="galM"
                     /locus_tag="ACHK74_003019"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFP0221794.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            13046..13798
                     /gene="gpmA"
                     /locus_tag="ACHK74_003020"
     CDS             13046..13798
                     /gene="gpmA"
                     /locus_tag="ACHK74_003020"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP0221795.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13956..15008)
                     /gene="aroG"
                     /locus_tag="ACHK74_003021"
     CDS             complement(13956..15008)
                     /gene="aroG"
                     /locus_tag="ACHK74_003021"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFP0221796.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            15324..15704
                     /gene="ybgS"
                     /locus_tag="ACHK74_003022"
     CDS             15324..15704
                     /gene="ybgS"
                     /locus_tag="ACHK74_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFP0221797.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGAMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15818..16759
                     /gene="zitB"
                     /locus_tag="ACHK74_003023"
     CDS             15818..16759
                     /gene="zitB"
                     /locus_tag="ACHK74_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFP0221798.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16756..17475)
                     /gene="pnuC"
                     /locus_tag="ACHK74_003024"
     CDS             complement(16756..17475)
                     /gene="pnuC"
                     /locus_tag="ACHK74_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFP0221799.1"
                     /translation="MDFFSVQSILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17513..18556)
                     /gene="nadA"
                     /locus_tag="ACHK74_003025"
     CDS             complement(17513..18556)
                     /gene="nadA"
                     /locus_tag="ACHK74_003025"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFP0221800.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18805..18880)
                     /locus_tag="ACHK74_003026"
     tRNA            complement(18805..18880)
                     /locus_tag="ACHK74_003026"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18845..18847),aa:Lys,seq:ttt)
     gene            complement(18914..18989)
                     /locus_tag="ACHK74_003027"
     tRNA            complement(18914..18989)
                     /locus_tag="ACHK74_003027"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18954..18956),aa:Lys,seq:ttt)
     gene            complement(19136..19211)
                     /locus_tag="ACHK74_003028"
     tRNA            complement(19136..19211)
                     /locus_tag="ACHK74_003028"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19176..19178),aa:Lys,seq:ttt)
     gene            complement(19215..19290)
                     /locus_tag="ACHK74_003029"
     tRNA            complement(19215..19290)
                     /locus_tag="ACHK74_003029"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19255..19257),aa:Val,seq:tac)
     gene            complement(19440..19515)
                     /locus_tag="ACHK74_003030"
     tRNA            complement(19440..19515)
                     /locus_tag="ACHK74_003030"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19480..19482),aa:Lys,seq:ttt)
     gene            complement(19680..20471)
                     /gene="cpoB"
                     /locus_tag="ACHK74_003031"
     CDS             complement(19680..20471)
                     /gene="cpoB"
                     /locus_tag="ACHK74_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFP0221801.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(20481..21002)
                     /gene="pal"
                     /locus_tag="ACHK74_003032"
     CDS             complement(20481..21002)
                     /gene="pal"
                     /locus_tag="ACHK74_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFP0221802.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(21037..22329)
                     /gene="tolB"
                     /locus_tag="ACHK74_003033"
     CDS             complement(21037..22329)
                     /gene="tolB"
                     /locus_tag="ACHK74_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFP0221803.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(22462..23616)
                     /gene="tolA"
                     /locus_tag="ACHK74_003034"
     CDS             complement(22462..23616)
                     /gene="tolA"
                     /locus_tag="ACHK74_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFP0221804.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGG
                     AKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAP
                     DGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(23681..24109)
                     /gene="tolR"
                     /locus_tag="ACHK74_003035"
     CDS             complement(23681..24109)
                     /gene="tolR"
                     /locus_tag="ACHK74_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFP0221805.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(24113..24805)
                     /gene="tolQ"
                     /locus_tag="ACHK74_003036"
     CDS             complement(24113..24805)
                     /gene="tolQ"
                     /locus_tag="ACHK74_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFP0221806.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(24802..25206)
                     /gene="ybgC"
                     /locus_tag="ACHK74_003037"
     CDS             complement(24802..25206)
                     /gene="ybgC"
                     /locus_tag="ACHK74_003037"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP0221807.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(25356..25649)
                     /gene="ybgE"
                     /locus_tag="ACHK74_003038"
     CDS             complement(25356..25649)
                     /gene="ybgE"
                     /locus_tag="ACHK74_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFP0221808.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(25649..25762)
                     /gene="cydX"
                     /locus_tag="ACHK74_003039"
     CDS             complement(25649..25762)
                     /gene="cydX"
                     /locus_tag="ACHK74_003039"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFP0221809.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(25777..26916)
                     /gene="cydB"
                     /locus_tag="ACHK74_003040"
     CDS             complement(25777..26916)
                     /gene="cydB"
                     /locus_tag="ACHK74_003040"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP0221810.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(26932..28500)
                     /gene="cydA"
                     /locus_tag="ACHK74_003041"
     CDS             complement(26932..28500)
                     /gene="cydA"
                     /locus_tag="ACHK74_003041"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFP0221811.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         7290 a         7643 c         7131 g         6981 t
ORIGIN      
        1 tgcagttaaa atttttaacg gccagccacc caaaatcgtc gcttcctttt tccggagtaa
       61 taatgaagca tgaattatca agtatgaagg catttgtcat actggcagag tccagttcat
      121 ttaacaatgc cgctaaatta ctcaatatta cgcaacctgc attaacgcga agaataaaaa
      181 agatggagga agatttacat attcaactat ttgagcgtac aactcgcaag gttacgttaa
      241 caaaagcagg gaaaaggttg ctcccggagg cgcgggaatt aataaagaaa ttcgatgaaa
      301 cgctttttaa tattcgtgat atgaatgctt atcatcgtgg tatggtgacg ttagcgtgta
      361 ttccgaccgc agtattttat tttttaccgc tggcaatcgg taaatttaat gagctgtatc
      421 ccaatattaa agtgcggatt ctggaacaag gcacgaataa ttgcatggaa tcggtgctgt
      481 gtaacgaatc agactttggc ataaacatga acaacgtcac aaattcatcc attgatttta
      541 ccccactggt caacgagcca ttcgtgctgg cctgtcggcg tgaccatcca ttggccaaaa
      601 agcagcttgt agaatggcag gagttagtgg gttacaaaat gattggcgta cgttcctcca
      661 gcggcaaccg attgttgata gaacaacagc tagccgacaa gccctggaag ctggactggt
      721 tttacgaagt gcgtcatctt tcgacgtcat taggactagt ggaggcagga ctggggattt
      781 cggcgcttcc tggtctggca atgccccatg cgccctattc ttcgattatt ggtatcccac
      841 tggtggaacc cgttatacgt cggacattgg gtattattcg ccgcaaagat gccgtgcttt
      901 ctccggcagc tgaacgcttt tttgccttgc tgattaattt gtggactgac gataaagaca
      961 atttgtggac caatatcgtc gaacgccaga gacatgcgtt acaggagata agttaataca
     1021 ccggcgcagg agagactgcg ccggttaaat cagcggttag tgtgcgttaa ccaccaccca
     1081 cattggtccc tgcccgaccg cataacggcc tttttcatgc agtagtccct gctcgccaac
     1141 aatttcgtat accgagatgt ggtgagattt ttgcccggcg gcaatcagat acttgccgct
     1201 gtgatcaaca ttgaagccgc gcggctgggt ttccgttggc tggaagcctt ctttactcaa
     1261 cacgctgcca tcttccgaaa cgctgaaaac ggtaatcagg ctggcggtac ggtcgcaggc
     1321 gtataaatgg cgaccatccg gggtgatatg aatatcagcc gcccaacggg tgtcggagaa
     1381 gttttccggc atcatatcca gcgtctggac acattcgata ttaccgtgcg gatctttcag
     1441 ttcccagaca tccactgagc tgtttaactc attgacgcaa tacgcatatt gttcgtttgg
     1501 atggaatacc atatgacgcg ggccggcccc ttcaacggtg gtcacttccg cagggtcctg
     1561 cgccacgaga tgaccatcat cgctgaccgt aaacaggcaa atgcgatcct gctttaatgc
     1621 cggaacccac agcgtacggt cgtccggtga gatattggcg gaatggcaac cgtccagccc
     1681 ctcgaccaca tcgacgacgc ccactggcag gccatcttcc agacgcgtta cgctcacgtt
     1741 acccgcattg taagaaccta caaagacaaa ctgcccctgg tgatcggtgg aaatatgcgt
     1801 cggactaccc ggcagcgcag actctgcggc aaaggtcagt gcgccatcgt ccggggcaat
     1861 acgatacgcc aggacgcgaa actcagggcg aacaccaaca tagagataac gtttgtccgg
     1921 gctgaccacc atcggctgca cctgccccgg cacatcgaca acctgtgtca gcgtcagtgc
     1981 gccttcatga ttcaggttcc agacgtgaat ttgctggctc tcagggctgg cgatataaac
     2041 tgtttgcttc atgaatgctc ctttgcattt agcaactgac tgtaacagct aaaattagtc
     2101 gcttttggcg gtgaatgctg aattaaaacg cagaattttg tccgatagtg gtatcggtgt
     2161 accattcgcg cagacgaaaa ttcttaacct taatctggaa tacgccatga ccacacgcgt
     2221 gattgctctc gacttagacg gcaccttatt gaccccgaaa aagaccctgc ttccttcatc
     2281 gatagaagcc ctggcccgcg ctcgcgaagc aggctatcaa ttaatcatcg tcacaggtcg
     2341 ccatcacgtc gctattcatc ctttttatca ggcgctggcg ctggatacac ctgctatttg
     2401 ctgtaatggc acctatttgt atgattatca tgcaaaaacc gtgctggaag cggacccaat
     2461 gcccgttaat aaagccctgc aactcattga gatgctgaat gaacaccaca ttcacggtct
     2521 gatgtatgtc gatgatgcaa tggtctatga gcacccgacc gggcatgtca ttcgcacgtc
     2581 taactgggcg caaaccctgc cgccggaaca gcgtccgact ttcacacaag tcgcttctct
     2641 ggctgaaacg gcgcaacaag ttaacgccgt atggaagttc gccctgacgc acgatgacct
     2701 gccgcaattg cagcattttg gtaagcatgt cgaacatgaa ctgggactgg agtgtgaatg
     2761 gtcctggcac gatcaggttg atattgcacg cggcggcaac agcaaaggta aacgtttgac
     2821 gaaatgggtt gaggcgcaag gttggtcgat ggaaaacgtc gtggcattcg gcgataactt
     2881 taatgatatc agtatgctgg aagccgctgg tacaggcgtg gcgatgggca acgccgatga
     2941 cgcggtaaaa gcgcgcgcca acattgtgat tggtgataac accaccgaca gcattgccca
     3001 gttcatttat agccacctga tttaatcagg cggttatcga cacactttta atttgcgcgt
     3061 acagccacag gccaggtttg atcgccagtt catccctggc ccacgggctg atacgcgccc
     3121 acagcgtttt accgccgact tccagctcca cttccacctg gccgttgtcg tcataactat
     3181 taacaacttt tgcccgcaat acgttacgaa tgctggtttg ctgcggcggt tgtaaaacca
     3241 gagaaacatc ggaagcctga atgcggatac gtagcgcagc ttgcagcggt tcgtccagct
     3301 tattgaccca caaatgctga tcgcccagcg ccagcgcagt catcgcgtaa tgcggatgat
     3361 gctcaagtac cgtcactttc agaatgctac tttgttgctc tttcggcagc cacggattca
     3421 tcacgctact accccagact tcttccagcg cgccaaaggc tttcacctga ccgttttcca
     3481 gtaccatcac tctgtctgcc agatggagga tctcatccag cgaatggctg acatacaaca
     3541 tcggaatgtt gatttcccgc gtcagacgtt gcagataagg caacagttcg cgtttacgcg
     3601 gaatatccag tgacgccagc ggttcatcca gcaacagcaa ttccggagcc gtcagcaaag
     3661 cccgaccaat cgccacgcgc tgtttttcgc ctccggacag gctgcccggt aaacggtcaa
     3721 gcaacggttc aatgcctaaa agcgccacca gcttatcgaa ctgatcgacc atgcttttcg
     3781 ccatgccgta gcgcaaattg ccacgcactt tgtaatgcgg gaacagccgt gcatcctgaa
     3841 aaacatagcc aacgcgacgt ttttctggcg tcaggcagat acctttttcg gcatcattta
     3901 gtacccgacc attgaggaca atcctccctt tttgcgggcg cgtcagtcca ctgatggcgt
     3961 taatcagcga agtttttccg gcaccggaga cgccaaagat agcagtgatg ccattggcgg
     4021 gcagcgtttc attaatagtc aggcaatggt tgcccaaggt ctgggaaaaa ttcagttcca
     4081 gcatgattag cgccccgccc gttcacggct gattctggcc agccattctg aaatcaacag
     4141 ggagatcatc gccagcgcaa tagaaataat gcacagtctc gctgcgccac tttccccgcc
     4201 gggggtctga atcagggtat acatggcaga aggaatggtt cgcgtttcac caggaatgtt
     4261 cgacacaaag gtgatggttg cgccaaattc accgagagaa cgagcaaaag ccagtaccgt
     4321 accaacaata attcccggta aggtcagcgg taacgtgata gtaaagaaaa cgcgccagcg
     4381 cccggccccc agtgttcttg cggcctgttc cagtttgaca tcaacccctt ccagcgccag
     4441 acgaattgcc cgcaccatta gcggaaacga catgacggcg gcagcgagaa ccgcgccgcg
     4501 ccagctaaag gcgaaggtaa taccaaacca gtcatacaga cgttcaccga taaatccgcg
     4561 ccgtcccatc gaaactaata ataagtaacc gacgaccacg ggcggtaaca ccagcggtag
     4621 atgcagtacg ctgtcgagca aagctttgcc cggaaacgtg caacgcacca gtaaccaggc
     4681 aaaaaagatc ccaaacggca ggctaaacag cacagccagg gaagaaactt tcaggcttaa
     4741 taaaactgcc tgccattctg gatcggtcag tatcattact tggttgtaaa tccgtaacgt
     4801 ttaaagattt ccgcagcctg cggtcccttc agataatcat aaaatgcttt cactgtcgca
     4861 ttgttatgcc cttccacaac agcaaccgga tattccactt ttttatgtga atcttccggg
     4921 aaggtggcaa ccacttttac ccctttgctg gcaactgcgt cagaaccgta aacaatgccc
     4981 aacggcgctt cattgcgttc gaccaacgcc agcgcgccac gaacatcttc cgccggagcc
     5041 agtttcgggg agagcgtatc ccatgcgccc agtttttgca gtgcttcttt tgcataaatg
     5101 ccagcaggga catgttccgg atcgccaacc gcgaggcgac cgccattcag cagcgaagtc
     5161 cagttagttt tgctgtcgat gatgaaatct ttctgctcgc tggctttcgg tgctacaacg
     5221 accaggctat tgccgagcag tgtctgacgc gtagctgtat cgatcgcttt tttatcaact
     5281 gcataatcca tccatttctg atcggcagaa ataaacagat ccgcaggcgc acccgcttca
     5341 atctgacggg cgagagtaga tgacgaagcg aaagaagaaa ccacatccac gcctttctct
     5401 tttttatact gcgtagcaat gtcctgcatt gcgttagtca gtgatgcggc ggcgaacacc
     5461 gtgattttcc cttcatctgc cagtgcattg ccagcaacag cgaaagagag tgccgccccg
     5521 gcaaacaagt tcaaccattt acgagccatt ggtaacccct taatgtaacg ttatgtaggc
     5581 gacaatataa cgactaactc ggaattttcc cagcagttat tactaacctt tgtgaggtag
     5641 ataagaaaaa atatcggcag gaaaagcagg aagttgagaa aaagaaaatg cccgactaag
     5701 cgggcattca gggaatcaat gattttgtcc gggctggtct tttttaccaa caccagaaaa
     5761 gatgttgaat acttcaccaa gaccgtaaat cagacccagg atgatggcca tcacgacagg
     5821 taccatgatt acggcgaata ccagactttt taataactct aacatggtca actccagata
     5881 tagtcatgag actattctaa ccgctaagca cagaaaagca ctcccctttt gtgcggtcag
     5941 ctttgcgtgg cgttcgtttt ccgtcacaat aagacttttg ccaggacatt gttatgcagg
     6001 ccgaaatcct tctcaccctt aagctccaac aaaaattatt cgccgacccg cgccgcattt
     6061 cgctactaaa acacattgcg ctttccggtt ccattagcca gggagcgaaa gatgccggta
     6121 tcagctataa aagcgcctgg gatgccatta acgagatgaa tcagttaagt gagcatattc
     6181 tggtcgagcg cgcaacaggc ggtaaaggtg gcggcggcgc agtactgacc cgctatggtc
     6241 agcgactgat tcagctctat gacttactgg cgcaaatcca gcaaaaagcc tttgatgtgt
     6301 taagtgacga tgacgccctg ccgctgaaca gcctgctggc cgcgatctca cgtttttcac
     6361 tgcaaaccag cgcccgtaac cagtggttcg gcaccatcac cgcccgcgat catgatgacg
     6421 ttcaacagca tgttgatgtc ttactggctg acggaaaaac acgcctgaaa gtcgcaatta
     6481 ccgcacaaag cggcgcacgt ctggggctgg atgaaggcaa agaagtgttg atattgctaa
     6541 aagcgccgtg ggtaggcatt actcaggacg aagcggtcgc acaaaacgcc gacaaccaat
     6601 taccgggtat tattagtcat attgagcgcg gcgcagagca gtgcgaagta ttaatggcgc
     6661 tacccgacgg gcaaacactg tgcgccacag tgccggttaa tgaagcgact tctcttcagc
     6721 aaggacagaa tgtcacggcc tactttaatg ccgacagcgt gattatcgcc acgctatgct
     6781 aagcgtgttg acaatttgtt atgaaacacg tatccctatc agtaatcgct gcacaaaatg
     6841 ggatataaaa tgtcatcgtt gcaaattttg caaggcacgt ttcgtcttag cgacacaaaa
     6901 acgctgcaat tgcctcagct aacgttaaac gcgggtgata gttgggcgtt tgtcggttcg
     6961 aatggaagtg gaaagtcggc gctggcccgt gcgctggcgg gggaacttcc gcttttgaaa
     7021 ggtgaacggc aaagccagtt ttcccacatc actcgtctct ccttcgagca attgcaaaag
     7081 ctcgtcagcg acgaatggca gcggaataac accgatatgc tcggccctgg cgaagatgac
     7141 accggacgca ctacggctga gatcattcag gatgaagtta aggatgcacc gcgttgcatg
     7201 caactggcgc agcagttcgg tattaccgcc ctcctcgacc gactctttaa atacctttcc
     7261 actggcgaga cgcgaaaaac cctgctgtgt caggcgctga tgtcggagcc tgacttgttg
     7321 attcttgatg agccgttcga tggcctggat gttgcctcac gtcagcagct ggctgagcga
     7381 ctcgcctcgt tacatcagtc cggtattact ctggtactgg tgctcaatcg cttcgatgag
     7441 atcccggaat ttgtccagtt tgctggcgtg ctggcggatt gtacgttagc ggaaacgggc
     7501 gctaaagagg aactgctcca gcaagcactc gtcgcgcaac tggcgcatag cgaacagctt
     7561 gaaggtgtgc aactgccgga gccggatgaa ccttcagcac gtcacgcctt acccaccaac
     7621 gaaccgcgca ttgtgctgaa taatggcgtg gtttcttata acgatcgccc cattcttaat
     7681 aaccttagct ggcaggtgaa tcctggcgag cactggcaaa ttgtcgggcc aaatggtgcg
     7741 ggaaaatcga cgttattaag cctgattact ggcgatcatc cgcaaggtta cagcaacgat
     7801 ttgacgcttt tcggccgacg tcgcggcagc ggcgaaacca tctgggatat caaaaagcat
     7861 atcggttacg tcagcagtag tttgcatctg gattaccggg tcagcactac cgtgcgtaat
     7921 gtgattcttt ctggctattt tgattcgatt ggcatttatc aggccgtttc ggatcgccag
     7981 caaaaactgg tgcagcagtg gctggatatt ctcggcattg ataaacgcac ggctgacgtt
     8041 ccgttccata gtctttcctg gggacagcag cgtctggcgc tgatcgtccg cgcgctggtg
     8101 aaacatccga cattgcttat tctcgatgaa ccactacagg ggcttgatcc gctcaatcgc
     8161 caacttatcc gccgttttgt tgatgtgctg attagcgaag gtgaaacgca attgttgttt
     8221 gtttcgcacc acgctgaaga tgcgcctgcc tgtattaccc atcgtcttga gttcgtgccg
     8281 gacggtgacc tttatcgcta tgcgctgaca aaaataaact gagttggtaa tgctgactgt
     8341 gccggatgtg gcgtaagccc cctctccggc ctacagttcg acgcatgcag gcatgaaacc
     8401 gcgtcttttt tcagataaaa agcgcaatca ttcataaacc ctctgtttta taatcactta
     8461 atcgcgcata aaaaacggct aaattcttgt gtaaacgatt ccactaattt attccatgtc
     8521 acacttttcg catctttgtt atgctatggt tatttcatac cataagccta atggagcgaa
     8581 ttatgagagt tctggttacc ggtggtagcg gttacattgg aagtcatacc tgtgtgcaat
     8641 tactgcaaaa cggtcatgat gtcatcattc ttgataacct ctgtaacagt aagcgcagcg
     8701 tactgcctgt tatcgagcgt ttaggcggca aacatccaac gtttgttgaa ggcgatattc
     8761 gtaacgaagc gttgatgacc gagatcctgc acgatcacgc tatcgacacc gtgatccact
     8821 tcgccgggct gaaagccgtt ggcgaatcgg tacaaaaacc gctggaatat tacgacaaca
     8881 atgtcaacgg cactctgcgc ctgattagcg ccatgcgcgc cgctaacgtc aaaaacttta
     8941 tttttagctc ctccgccacc gtttatggcg atcagcccaa aattccatac gttgaaagct
     9001 tcccgaccgg cacaccgcaa agcccttatg gcaaaagcaa attgatggtg gaacagatcc
     9061 tcaccgacct gcaaaaagcc cagccggact ggagcattgc cctgctgcgc tacttcaacc
     9121 cggttggcgc gcatccgtcg ggcgatatgg gtgaagatcc gcaaggcatt ccgaataacc
     9181 tgatgccata catcgcccag gttgctgtag gccgtcgcga ctcgctggcg atttttggta
     9241 acgattatcc gaccgaagat ggtactggcg tacgcgatta catccacgta atggatctgg
     9301 cggacggtca cgtcgtggcg atggaaaaac tggcgaacaa gccaggcgta cacatctaca
     9361 acctcggcgc tggcgtaggc agcagcgtgc tggacgtggt taatgccttc agcaaagcct
     9421 gcggcaaacc ggttaactat cattttgcac cgcgtcgcga gggcgacctt ccggcctact
     9481 gggcggacgc cagcaaagcc gatcgcgaac tgaactggcg cgtaacgcgc acactcgatg
     9541 aaatggcgca ggacacctgg cactggcagt cacgccatcc acagggatat cccgattaag
     9601 gaacgaccat gacgcaattt aatcccgttg atcatccaca tcgccgctac aacccgctca
     9661 ccgggcaatg gattctggtt tcaccgcacc gcgctaagcg cccctggcag ggggcgcagg
     9721 aaacgccagc caaacaggtg ttacctgcgc acgatccaga ttgcttcctc tgcgcaggta
     9781 atgtgcgggt gacaggcgat aaaaaccccg attacaccgg gacttacgtt ttcactaatg
     9841 attttgcggc cttgatgtct gacacgccag atgcgccaga aagcaacgat cctttgatgc
     9901 gttgccagag cgcgcgcggt accagccggg tgatctgctt ttcaccggat cacagtaaaa
     9961 cgttgccaga actgagcgtt gcggcattga cggaaatcgt caaaacctgg caggagcaaa
    10021 ccgcagagct gggaaaaacg tacccgtggg tgcaggtctt tgaaaacaaa ggtgcggcga
    10081 tgggctgctc taacccgcat ccgcacggac aggtttgggc aaatagcttc ctgcctaacg
    10141 aagctgagcg cgaagaccgc ctgcaaaaag aatattttgc cgggcagaaa tcgccaatgc
    10201 tggtggatta tgttcagcgc gagctggcag acggtagccg taccgttgtc gaaaccgaac
    10261 actggttagc cgtagtgcct tactgggctg cctggccgtt cgaaacgcta ctgctgccca
    10321 aagcccacgt tttacggatc accgttttga ccgatgccca gcgcagcgat ttagcactgg
    10381 cgttgaaaaa gctgaccagt cgttatgaca acctcttcca gtgctccttc ccctactcta
    10441 tgggctggca cggcgcgcca tttaatggcg aagagaatca acactggcag ctgcacgcgc
    10501 acttttatcc gcctctgttg cgctccgcca ccgtacgtaa atttatggtt ggttatgaaa
    10561 tgctggcaga gacccagcga gacctgaccg cagaacaggc agcagagcgt ttgcgcgcag
    10621 tcagcgatat ccattttcgc gaatccggag tgtaagaaat gagtctgaaa gaaaaaacac
    10681 aatctctgtt tgccaacgca tttggctacc ctgccactca caccattcag gcgcctggcc
    10741 gcgtgaattt gattggtgaa cacaccgact acaacgacgg tttcgttctg ccctgcgcga
    10801 ttgattatca aaccgtgatc agctgtgcgc cacgcgatga ccgtaaagtt cgcgtaatgg
    10861 cagccgatta tgaaaatcag ctcgacgagt tttccctcga tgcgcccatt gtcgcgcatg
    10921 aaaactatca atgggcgaac tacgttcgtg gcgtggtgaa acatctgcaa ctgcgtaaca
    10981 acagcttcgg cggtgtggac atggtgatca gcggcaatgt gccgcagggg gccgggttaa
    11041 gttcttccgc ttcactggaa gtcgcggtcg gaaccgtatt gcagcagctt tatcatctgc
    11101 cgctggacgg cgcacaaatc gcgcttaatg gtcaggaagc agaaaaccag tttgttggct
    11161 gtaactgcgg gatcatggat cagctaattt ccgcgctcgg caagaaagat catgccttgc
    11221 tgattgactg ccgctcactg gggaccaaag cagtttccat gccgaaaggc gtagctgtgg
    11281 tcatcatcaa cagtaacttc aaacgtaccc tggttggcag tgagtacaat actcgccgtg
    11341 agcagtgcga aaccggtgcg cgtttcttcc agcagccagc tctgcgcgat gtcaccattg
    11401 aagagttcaa cgctgttgcg catgaactgg acccgatcgt ggcgaaacgc gtgcgtcata
    11461 tcctgaccga aaacgcccgc accgttgaag ctgccagcgc gctggagcaa ggcgacctga
    11521 aacgtatggg cgagttaatg gcggagtctc atgcctctat gcgcgatgat ttcgaaatca
    11581 ccgtgccgca aattgacact ctggtagaaa tcgtcaaagc tgtgattggt gacaaaggtg
    11641 gcgtacgcat gaccggcggc ggatttggtg gctgtatcgt cgcgttgatc ccggaagaac
    11701 tggtgcctgc cgtacagcaa gctgtcgctg aacaatatga agcaaaaaca ggtattaaag
    11761 agacttttta cgtttgtaaa ccatcacaag gagcaggaca gtgctgaacg aaactcccgc
    11821 actggcaccc gatggtcagc cgtaccgact gttaactttg cgtaacaacg cagggatggt
    11881 agtcacgctg atggactggg gtgcgacttt actttccgcc cgtattccgc tttccgatgg
    11941 cagcgtccgc gaggcgctgc tcggctgtgc cagcccggaa tgctatcagg atcaggctgc
    12001 gtttctgggg gcctctattg gtcgttatgc caaccgcatc gccaatagcc gttatacctt
    12061 tgacggtgaa accgtgacgc tttcgccaag tcagggcgtt aaccagctgc acggcggccc
    12121 ggaagggttc gacaaacgtc gctggcagat tgtgaaccag aacgatcgtc aggtgctgtt
    12181 tgccctgagt tcagatgatg gcgatcaggg cttcccgggt aatctcggcg cgacggtgca
    12241 atatcgtctg accgacgata accgtatctc tattacttat cgcgccacag ttgataaacc
    12301 ttgcccggtg aatatgacta atcacgtcta tttcaatctt gatggcgagc agtctgacgt
    12361 gcgcaatcac aagttgcaga ttctggcgga cgaatatctg ccggttgatg aaggcggcat
    12421 tccgcacgac ggcctgaaat ctgtcgccgg aacgtctttt gatttccgta gcgccaaaat
    12481 catcgccagt gagtttcttg ccgacgacga tcagcgcaaa gtgaaaggtt acgatcacgc
    12541 attcttgtta caggccaaag gcgatggcaa gaaagtggcg gcgcatgtct ggtcagcaga
    12601 tgaaaaattg cagctgaagg tctacaccac cgctccggct ctgcaattct actccggcaa
    12661 cttcctcggc ggcacgccat caagaggaac tgaaccttac gccgactggc aaggcctggc
    12721 actggaaagc gagtttctgc cggacagccc aaaccaccct gaatggccgc aaccggactg
    12781 cttcctgcgt cctggcgaag agtattccag cctgacggaa tatcagttta ttgctgagta
    12841 gcacctgaag ccctcgtaat gagggcttta tccttatatt gctcaaatca ccagcaaaca
    12901 ccgacatatt tgcaactcaa tattcacaac aaccttacac tgcgacacta ttttcgctat
    12961 ggttatgcgt aagcattgct gttgcttcgt cacggcaata taatgagaat tattatcatt
    13021 aaaaaatgat ttgaggagta agtatatggc tgtaactaag ctggttctgg ttcgtcatgg
    13081 cgaaagtcag tggaacaaag aaaaccgttt caccggttgg tacgacgtgg atctgtctga
    13141 gaaaggcgta agcgaagcaa aagcagcagg taagctgctg aaagaggaag gttacagctt
    13201 tgactttgct tacacctctg tgctgaaacg cgctatccat accctgtgga atgtgctgga
    13261 cgaactggat caggcatggc tgcccgttga gaaatcctgg aaactgaacg aacgtcacta
    13321 cggtgcgttg cagggtctga acaaagcgga aactgctgaa aagtatggcg acgagcaggt
    13381 gaaacagtgg cgtcgtggtt ttgcagtgac tccgccggaa ctgactaaag atgatgagcg
    13441 ttatccgggt cacgatccgc gttacgcgaa actgagcgaa aaagaactgc cgctgacgga
    13501 aagcctggcg ctgaccattg accgcgtgat cccttactgg aatgaaacta ttctgccgcg
    13561 tatgaagagc ggtgagcgcg tgatcatcgc tgcacacggt aactctttac gtgcgctggt
    13621 gaaatatctt gataacatga gcgaagaaga gattcttgag cttaatatcc cgactggcgt
    13681 gccgctggtg tatgagttcg acgagaattt caaaccgctg aaacgctatt atctgggtaa
    13741 tgctgacgag atcgcagcga aagcagcggc ggttgcaaac cagggtaaag cgaagtaaac
    13801 gtcattcgtt taaaatgaga aagccgactg caagtgagtc ggcatttttg ttgctaacaa
    13861 tggagcacat tgcctgatgc gacgctgcgc gacttatcag gcctgtggtg attcatcgga
    13921 tacgccactc tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc ttttactgca
    13981 ttcgccagtt gacgtaacag agcatcggta tcttcccagc cgatgcaggc atcggtgatg
    14041 ctcttaccgt aggccagcgg ctccccgctc tcgaggctct gattgccttc caccagatgg
    14101 ctttccacca tcacgccaat aatggccttt tcgccaccgg caatctgctg gcaaacgtca
    14161 gcacaaacat ccatctgctt tttgaattgt ttggacgagt tagcatggct gaaatcgatc
    14221 atcacctgtg ctggcaggcc tgctttgttc agcccttctt tcacttcagc aacgtgcttc
    14281 gcgctgtagt taggctcttt accgccgcgc agaatgatat ggcaatcgcc gttaccgctg
    14341 gtattcacaa tcgccgaatg cccccatttc gttacggaca ggaagcagtg cggcgcaccg
    14401 gcggcattaa tggcatcgat agccacttta atcgtaccgt cggtgccatt tttgaagccg
    14461 accggacaag aaagccctga tgccagttcg cggtgcacct gcgattcggt ggtacgtgcg
    14521 ccaattgcgc cccagctcat caggtcagcg agatattgtg gggtgatcat atcgagaaac
    14581 tcacctgccg ctggcagacc gctgtcgtta atatcaagca gcaatttacg ggctatacgc
    14641 agaccgtcgt tgatctggaa gctattatcc atatgcggat cgttaatcag ccctttccag
    14701 cccaccgtgg tacgcggctt ttcaaaatag acgcgcatta cgatttccag ctcatctttc
    14761 agctcttcac gcagcgccag caagcgagtg gcatactctt ttgccgcgac aggatcatga
    14821 attgagcatg ggccaatcac aaccaacagg cgatcatcat tacctttcag gatcttatgg
    14881 atcgcttttc gggcatgggc aaccgtattc gcggcatttt cagtagcggg gaatttttcc
    14941 agcaatgcga caggaggaag taactctttg atttctttga tgcgtaaatc gtcgttctga
    15001 taattcatgt ctgttccagt gttgccatac ttatcttagt gaatgcaata cttccaatct
    15061 atatcttccg tcagaatgtg taaacggggt tttacactat gaacggatta atcctggaat
    15121 tagcaagaaa aacgccagat tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt
    15181 aactcctttg tttgttaatt atttcaagat tctctgctgc gtttcataac ctggctgaaa
    15241 tcttaaacca atgccttata ttcacctgca aatgcactgt tggaagaggt tatccgacat
    15301 aacgaccata acaggagcat cctatgaaaa tgacaaaact ggccacatta tttctgactg
    15361 ccactctaag ccttgccagc ggtgccgcac tggctgccga tagcggagcg caaactaata
    15421 acggccaggc aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg
    15481 tcgcgccaaa taacgtcgac aataacgggg taaataccgg ttctggtggc gcaatgctgc
    15541 attcggatgg ttcttcaatg aacaatgacg gaatgaccaa agatgaagag cacaagaaca
    15601 ccatgtgcaa agatggtcgc tgtccggata tcaataaaaa ggtacaaacc ggtgatggca
    15661 tcaataatga tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca
    15721 ataatgggag agcggagtct ctccctttta atttttcact tctaaaagca gtatgatgtc
    15781 ataaagttaa gtgagaataa ttaagggatg actcgtcatg gcgcactcac actcacacac
    15841 gtcctcacac ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac
    15901 tgccgggttt atgctggtag aagtcgttgg tggttttctt tctggttctc tggcattgct
    15961 ggccgatgcg ggtcatatgt tgaccgatac tgccgccctg cttttcgccc tactcgccgt
    16021 gcaattttca cgtcgtcctc ccaccattcg ccacactttc ggttggctaa gactgaccac
    16081 gctcgcggcg tttgtgaatg ccatagccct cgtggtaatt accattttga ttgtctggga
    16141 ggcgatagaa cgcttccgca cgccgcgtcc ggtcgagggc ggcatgatga tggcaattgc
    16201 cgtggctggg ttgctggcaa atatactttc tttctggtta cttcatcacg gcagcgaaga
    16261 gaaaaacctc aacgtacgag cagcggcact gcatgtgctc ggggacctgc tgggttcggt
    16321 tggggctatt attgccgcgt tgattattat ctggactggc tggacacctg ctgaccccat
    16381 tctctcgata ctggtgtcgc ttctggttct gcgtagcgcg tggcgactgt tgaaagatag
    16441 cgtgaatgaa ttacttgaag gtgcaccggt atcgctggat attgctgaac tgaagcgtcg
    16501 tatgtgccgg gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg
    16561 cgagaagccg gtgatgacgt tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt
    16621 gttggatcag atccaacact acctgatgga tcactatcag attgagcacg ccaccattca
    16681 gatggaatat caaccttgtc atgggccaga ctgccacctg aatgaagggg tgtctggcca
    16741 ttcacatcat caccattaat gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat
    16801 ccacatccgg ctgccgttga gcgcaataaa ggtcaggatg atgtactcca gagacatggc
    16861 gtaaacgccc tgaagtgcaa aaataacgac gctaatcacg ttaataatca cccacaacag
    16921 ccagttttcc acatacttac gcgtcatcag aatcattgcc acgatagata acaccatcat
    16981 gcatgaatcc cagaacggga aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc
    17041 taatgcttgc atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa
    17101 taccgtcatc agaccaatcg aaacaacgca aaccgccaac cagctgagtg ccttcggcaa
    17161 tggcaaccag cgaattttca actccgcctc gttctgactg gtttgtcgcg accacgcata
    17221 ccaaccgtaa atattcgcgg caaagaaaaa cacctgtaat agcaggctgg catacagctg
    17281 aatctgaaag aaaataatgc caaacaaggt gacgttgatc aggccaaaga agtagttgct
    17341 gatcttctcc agactggcaa ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc
    17401 gatccatgag agatcataac cgcctgcccc tattggtata tgtaccagga tactctgcac
    17461 actaaaaaaa tccatctttc ccccaaagcg taacgttacg ccttattatt cgttatccac
    17521 gtagtgtagc cgcaaaatcc agcatacgat tgagcggcac cagcgccctc tctcgcagcc
    17581 tttcatcaac atgaacctcg tgattgcttc cttcctgttc taatgcctct gcgatggact
    17641 gaaggccatt catggccatc cacggacaat gcgcgcagct gcggcaggtt gcaccctcac
    17701 ctgcggttgg tgcttccagt aactctttat ctggcactgc ctgctgcatt ttgtagaaaa
    17761 tgccccgatc ggttgccacg ataagccgct gatgtggcaa tgttttcgca gcagcgatca
    17821 gttgactggt ggaaccgacc gcatccgcca tatcgacaat agcttgtggt gattctggat
    17881 gcaccagtat ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa
    17941 attcatcatg cacaatacag gcaccctgcc agcatagaat gtctccaccc gtctgttttt
    18001 gcacgtaacg ccccagatgt ttgtcgggtg cccagatgat tttttcaccc aaactatcaa
    18061 gatgatcaat aagttcgacg gcaatgcttg aagttaccac ccaatctgcg cgcgctttta
    18121 ccgcagcaga agtgttggcg tagacgacga cagtacgatc gggatgggca tcgcaaaatg
    18181 cgttaaattc ttcaacaggg cagccgagat ccagtgaaca ttcagcctga agtgtcggca
    18241 tcagaattgt tttttccgga ctgagaattt tggcggtttc tcccataaat ctcaccccag
    18301 cgactaacaa agtagaagcg ggatgctttg caccgaagcg cgccatttcc agagaatcag
    18361 aaatacagcc accggtttct tctgccagtt gttgaatttc gggatcggta tagtagtggg
    18421 caaccatcac cgcattacgt tcttttagca gacgttttat cttctcgcgg taatacgctt
    18481 tttcatcaat gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat
    18541 caaacattac gctcatctta ccatctcgtt ttacaggctt aacgttaaaa ccgacattag
    18601 cgtaatattc gctgttatcg gtgtaatttg ttttatatgc taaacaagat agtcgaatgg
    18661 tggggaaaag tcactcgaat tacgcctaat tccaccgacg tagagttgct tgcacagtaa
    18721 gctctgacga tgtcatcagg tttacaaagt gaaattgagg atgcctgaat agcaaaaaag
    18781 cgcctttagg gcgctttttt acattggtgg gtcgtgcagg attcgaacct gcgaccaatt
    18841 gattaaaagt caactgctct accaactgag ctaacgaccc cttgcgggat tatgctgctt
    18901 taatcattca ggatggtggg tcgtgcagga ttcgaacctg cgaccaattg attaaaagtc
    18961 aactgctcta ccaactgagc taacgaccca tacgggtgtt acctgaaaga tttctactcg
    19021 gggccaatct ttaagattgg tgtgtcgtgc aggatgactc gcttcgcttg ccctacgggc
    19081 cgttgctggc gcaacgttat ccttcacgct taacatctga gtttgatgtt aaaattggtg
    19141 ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag tcaactgctc taccaactga
    19201 gctaacgacc cgagtggtgg gtgatgacgg gatcgaaccg ccgaccccct ccttgtaagg
    19261 gaggtgctct cccagctgag ctaatcaccc gatactgcgc tggatactac aaactacttc
    19321 caccgaaccc accgtaatta gtggtgggtc gtgcaggatg actcgcttcg ctcgccctac
    19381 gggccgttgc tgacgcaacg ttatcctaca cgcttaacat ctgtcttcga tgctaaaact
    19441 ggtgggtcgt gcaggattcg aacctgcgac caattgatta aaagtcaact gctctaccaa
    19501 ctgagctaac gacccacttt ttcgttgctt tcggtttgtt tgatatcccg tggcaacggc
    19561 ggcatatatt actgatttca gacttgagcg caacaaaaat ttcgatgcag atcactcaac
    19621 tgcttatgat tcgcacgaca cgaccagaaa taatgcgact tctggtcgtg tgttatgcat
    19681 tacatcgcgt tcagacgttt ttgtgcctgt ttagcgccat cagtaccagg gtatttactg
    19741 ataacctgct ggtatacggc tttcgctttt gcggtgtcac ctttgtcctg catgatgacg
    19801 ccgactttaa acatcgcatc agcagccttt ggtgacttcg gatagttttt cactaccgaa
    19861 gcaaaatagt acgccgcatc atctttttta cccttgttgt agtttaactg acccagccaa
    19921 taattggcgt ttggcaggta agttgaatca gggtaatttt tgatgaaatt ctgaaatgcc
    19981 accattgcgt catcctggcg ggatttatcc tgcaccagcg caatagctgc attgtaatcc
    20041 gtgtttgcat cgccgctttt caccggcgcg ccagcattcg cagtaccagc atcagctgtc
    20101 ggcgtcgttg atgccgccgc accgctttga tcgctgctgg ttgattgcgc cgctgcacca
    20161 ccgctgctga ggctgtcgat ctgcaacagg atctgctttt gccgctccac gacctgattc
    20221 agttgatact gattttcctg aatctgacca cgcagggaat caatatcgga ttgattatca
    20281 gaaagttgtt gctggagttg ggttaaaagc tggctgtgag cattagaaat acgctcaagt
    20341 tgagtgacgc ggtcttcgac cgagcctgag ccgacactac tgattggtgc ctgagcaaaa
    20401 gcggcccagg gggccgctat accaaccagt aacgacagac tcaatagttg atgtctgaag
    20461 ttactgctca tgcaattctc ttagtaaacc agtaccgcac gacggttttt ggagtatgcc
    20521 gcttcgtcat gacccagtac tgcaggtttt tctttaccgt aagaaacgat ggagatctgg
    20581 tctgcagaaa cgcctttacc ctgcaggtac atcttaacgg cgttcgcacg acgttcaccc
    20641 agggagatgt tgtattccgg agtaccacgt tcgtccgcgt gaccttctac ggtgactttg
    20701 taagacgggt tgctacgcag gaagtttgca tgtgcatcca gcatttgagc gaagtcagaa
    20761 cggatatcgt acttgtccag atcgaagtaa acgatgttgt tctgctgcag ctgttgcatt
    20821 tgcagacgag cctgctcttc ggaagacatg ttgccgttgc cgccgttcgc atccatacca
    20881 gtgccggcac ccagcatgcc ttcgctgccg tcattgctgg cgttcttgtt ggaagaacat
    20941 gccgcaattg ccataacagg cagagcaatc atcagccctt tcagcacttt gttcagttgc
    21001 atttcaatga ttcctttact attcaattaa ttattatcac agatacggcg accaggcagg
    21061 gaatttgacc tgtccatcag ttgccggaag acgcgctttg aaacgcccat ctgtagaaac
    21121 caaattcagc acggatccca tcccctgaga agagctgtag attaccatag tgccgttagg
    21181 tgccagactt ggcgtttcat ccaggaacgt ggacgacaga acttgtacgc ctcccgttgc
    21241 cagatcttgt ttggcaatgt gctgctgccc accattggag ctgaccatta ccataaattt
    21301 accgtcgctg ctgacatccg catcctggtt ctgcgaacct tcccaggtaa tacgttgtgg
    21361 cgcaccgccg ttgatattca ctttataaac ctgcggacga ccggcctggt cagaagtaaa
    21421 tgccaggttc tggctatccg ggaaccaggt cggttcggta ttgttactgc gaccatcagt
    21481 cacctggcgg atctgaccag aagccaaatc cattacgtac aggttcagac taccggtttt
    21541 cgacaaggcg aatgccagtt tgctgccgtc tggcgagaat gcaggtgcac cgttgtgacg
    21601 cgggaatgaa gccacctgac gtacagcgcc atttgccagc gtctgaataa ccagcgcgga
    21661 acgaccgctt tcgaaggtca cataagccag tttagaaccg tctggtgacc acgccggaga
    21721 catcagcggc tgcggtgaac ggtgaacgac aaactggttg taaccgtcat agtcagatac
    21781 gcgcagttca tacgggaact gaccgccgtt ggtctgaaca acgtaggcaa tacgagtacg
    21841 gaacgcacct ttaatgccgg tcagtttttc aaacacttca tcactggcgg tatgaccagc
    21901 ataacgcagc cactgcttgt tcactttgta cgagttctga gcaagtacag tacccggtgc
    21961 gccgccagtg tcaacaagtt gataagcaac attgtaagaa ccatccggat tcggagtgac
    22021 ctgaccgaca actacagcgt caatgcccag tgcggaccat gcagctggtt gtacttcctg
    22081 cgcactaccc ggctgctgtg gcagacgagc gcgatctaac ggattaaatt taccgctgtt
    22141 acgcaagtca gcagcaacga tgccgccaat atcttcaggt gccgcaccag gccccgccca
    22201 ctggaaagga acaacaccaa taggacgacc ggaatctaca ccgctgtcga tcacaatgcg
    22261 gacttcagca tgcagaactg atgcccacag tatgagaaaa ccaaatgcta ctcgtaatgc
    22321 ctgcttcatc atatctccct tatctggacc gatggcccac gataatttag cagaatgtta
    22381 acaaactaaa atacacaaaa ctaccagaac cccgtggcaa ccggtgcctg atgttgaccg
    22441 tccgaacagt caacatcgcg attacggttt gaagtccaat ggcgcgtttt tgaacacttc
    22501 atatactgcc tggcttggtg gtttcgggat cttcgcaagt ttagctgctg ccaacgcagc
    22561 ctgacaaagt gcgggatcgc caccttcagg tttgatatcc agtaacatac catcgggtgc
    22621 cagttttatg cgcagcgtac aggttttgcc tgcataggac gatgcgtcat agaacttact
    22681 ttcgatagca gatttaatct gcccggcata gttattgata tcggcccctg atgcgccatt
    22741 gtttttagta ttaccactcc cggcaggcga agcattgttc cctttcgccc ctccccccgt
    22801 tttcggtgca ttcttaccag agcttagctc accgaaaata tcatctgcct ctgcggccgc
    22861 ttttgctgca gcggcttttt cagctgcagc ttttgcagct gccgctttct tatcagctgc
    22921 agccttttca gcagccgctt tcttctcagc ttctgctttg gctttttcag cagcttcagt
    22981 tgccgctttc tttcttgctt cagctgcagc tgcttctgcc gcttccgctt tcttcttcag
    23041 tgccgcagcg gctgcctcgg cttttttctg cgcttcggct gcggctttgg cggcttctgc
    23101 ttctgctttt ttctttgcgt ctgcagccgc tttcttcgct gcttcttccg cagctttagc
    23161 atctgcttcg gccttcgctt tagcatctgc cgccgctttc gctgccgcct cttcagcttg
    23221 cttctgcttt aactcggcct gttttgcggc ttcttcagcc tgctttttct gctcctgagc
    23281 cgctaaccgc tctttctcaa gttgcttcag gcgttcctgt tcagccgctt gtttctcacg
    23341 cagttcttca gcagcctgct gttccttcat cttgcgctgt tcatcagaac gcttcgcgct
    23401 tgattcctgg ctttgcatgc gtttgtactg ctcaactacc gcacctgaat caaccatgac
    23461 agcgtcgatg gacgaaccac cgccgcctcc ggctgaagct tctatattct catcgaacga
    23521 actccagatc agcgccgcaa ataagatgac atgcagcact gctgaaataa ttatcgcccg
    23581 cttgagcttg tcgttttgtt cggttgcctt tgacactctc ggtttccaaa aactgttcgc
    23641 ctgttacccg ctctctttca agcaagggaa acgcagatgt ttagataggc tgcgtcatta
    23701 aaccaaccga tttcacaccc gcactatgta acaagttcag tgctttaatt atttcatcgt
    23761 aaggcacatc ttttgcgcca ccgatcagaa agaccgtttt cgggttggcc ttgaaacggc
    23821 tggacacttc cgccaccacc tgctctggtg gtaaacgctc caggcgatct ttctcaacca
    23881 ccacggtgta ctgaccaata ccagacactt caacaatcac tggcggatta tcgttactgc
    23941 tcaccgcctg tgattcagta gcgtctggca gatcgacctc cacgctctgg gtgatgatgg
    24001 gcgctgtcgc cataaagatc agcaacagca ccagcagtac gtccagcaac ggtacaatgt
    24061 tgatttcgga cttgagatcg cgacgacctc gtccacgcgc tctggccatg gcttacccct
    24121 tgttgctctc gctaacggta aacgcctggc ggtgcagaat cgcggtaaac tcttccataa
    24181 agttgtcgta attcagttcc agtttgttta cgcgctggtt gaggcggtta taagccatta
    24241 ccgccggaat tgcggcgaac agaccaattg cagtcgcaat caacgcttct gcgataccgg
    24301 gcgcaaccat ttgcagtgtt gcttgtttta ccgccccgag ggcgataaag gcgtgcatga
    24361 tcccccagac cgtaccaaac agaccaatat acgggctgat ggagccaacc gtaccgagga
    24421 acggaatgtg cgtttccaga ttttcaagtt cacggttcat ggagatacgc atagcacgcg
    24481 acgccccttc cactacggct tccggcgcat ggctattggc acgatgcagg cgcacaaact
    24541 ctttgaaccc gctgtaaaag atttgttccg aaccagtcag attatcccgt ttcccctggc
    24601 tctcttgata gaggcgagag agttcgattc cagaccagaa tttatcttca aacgcttcgg
    24661 cttcgcgcgc cgctgcgtta agaatacggg tccgctggat aataatggcc caagatgcga
    24721 ttgaaaaacc aatcaaaatc aacatgataa gtttaaccag aaggctagcc ttcaggaaca
    24781 aatcaaggat attcatgtca gtcactgctt aaactccgcg acaatagact tgggaagcgc
    24841 acgaggcttc attttgagtg ggtcaacgca aacaaccaga acctctgctt cattcagcaa
    24901 agtattctcg gcgttgacaa tacgttgcgt gaaaaccaaa gaggtgccac gcattgatgt
    24961 tatttcagtc tgtatttcga gcatatcgtc gagccgcgca ggtgcgtaat attccaccgt
    25021 cattttacgt accacaaagg caacgcgttc agccatcagc gcctgctgac tgaagtgatg
    25081 atgacgcagc atctctgtgc gtgctctttc ataaaaagcg acgtaactgg cgtggtacac
    25141 cacaccaccg gcatcggtat cttcatagta gacgcgaacc ggccatcgaa acagcgttgt
    25201 attcacttta catcccggta atgcaacaaa agttagagct tttaaacgct gctactatac
    25261 gcgcgcgatt gaggtttggg aatgagggca agttaaggga gcgtaaaaat ttatgggcct
    25321 ggagcggccc ataatttttt aaagagaatt ctgatttaaa agaagaagaa aatcagcccg
    25381 acaatgagaa caatatcggc aagcaatggg caaaaaatcc cttgccaaag aaccttctgc
    25441 ggacgaaagc ccacgccgtg aatcacacca gcacagacgg cccacatcag caataaacca
    25501 tgccagattt ccagttcact ggtcttcgcg gcaaaacgag atgggtccca aaacatacat
    25561 cctgctaaca gaagcgccat cacgaaggaa agcgcccgca gggggcgctt gtccattacc
    25621 gcatacaaag tcgcgataat cttactcatc agatgtcttc ttgaccggct ttgcctgatt
    25681 cgacgtgttc aagcgccagc gcggtgatta ccccaaacga acaggcaaga agcgttccca
    25741 gaatccatgc gaaataccac atttttagct ccttacttag tacagagagt gggtgttacg
    25801 ttcaatatct tctttggtga tacgaccgaa cattttccag taacaccagg cggtgtagag
    25861 cagaatgatc ggtaccagaa ccaccgcaac ccaggtcatg acgttaagcg tcagctggct
    25921 ggaagttgca tcccacattg tcagacttgc gttcatcatg gtgctggacg gcatcacaaa
    25981 cgggaacatt gcgataccgg ctgtcaggat gatgcaggcc agcgtcaggg aggagaacac
    26041 aaacgcccac gcggctttat ccatacgtgc agtcaggatg gtcagcagcg gcagaaccac
    26101 acccagtgcc ggaatagccc acagaattgg cgtgttgttg aagttaacca gccatgcgcc
    26161 agcttcacga accacttctt tattcagtgg gttagaggct gcgtaatggt ccattgtcga
    26221 tttcacgaca taaccatcga taccgtacat cacccatacg ccagccagtg cgaaacagac
    26281 cagtgtcacc agcgcagcca cctgagccgt tgcacgggta cgcaggtgca gttcgcccac
    26341 ggtacgcatt tgcagatagg ttgcgccctg agtaatgatc atccctacgc tcaccacgcc
    26401 tgccagcagg ccgaacgggt taagcaactg gaagaagtta ccggtgtagt acagacgcag
    26461 atattcatca acgttgaacg gtacgccctg caacaggtta ccgaacgcta caccaattac
    26521 cagcggcgga acgaagctac caatgaagat gccccagtcc cacatgttac gccagcgggt
    26581 ttcttcaatc ttggagcggt agtcaaaacc gaccggacgg aagaacaaag acgccagcac
    26641 gaggatcatc gccacataga agccggagaa cgcagcggca tagaccatcg gccaggcagc
    26701 aaagagtgcg ccgcccgcgg tgatcagcca aacctggtta ccgtcccagt gtggtgcaat
    26761 ggagttaatc ataattcgac gctcggtgtc gttacgaccg aggaaacggg tgagcatgcc
    26821 cacccccatg tcgaaaccgt cagtgactgc aaaaccaatc agcagaacgc caaccagcag
    26881 ccaccagata aaacgcaata cttcataatc gatcatttga cgactcctgt cttagcgtgc
    26941 cggctgagta gtcgtggaag actgctcaaa gtgatagcga ccggttttca ggctgcttgg
    27001 gccgaggcgt gcaaatttga acattaagaa caattctgcc accaggaaca gggtatacag
    27061 gccgcaaatc agcaccattg agaagatgag atcgcctgcg gtcagtgacg agttcgccac
    27121 agctgtcggc agcacttcac cgatagccca cggttggcgg ccatattcag ccacgaacca
    27181 gcccgcttct acagcaatcc acggcagcgg aataccgtac agcgcggcgc gcagaagcca
    27241 ttttttctcg ccaatgcggt tgcggatgac actccagaaa gagagcgcga tgattgccag
    27301 aagcaggaag ccacacgcca ccatgatacg gaacgcaaag tacagcggcg ctacacgcgg
    27361 gatggagtct ttggttgcct gttgaatctg cgcttcagtc gcatcagcca cgtttggcgt
    27421 atagcgtttc agcagcagac cgtaaccgag gtctttcttc atgctattga actggtcacg
    27481 aaccgcctgg tcggtagaac cagaacgcag ttgttcgagc agagagtacg ccttcatccc
    27541 gttacgaatg cgttcttcat gctgcaccat cagctctttc aggccgataa ccggggtatc
    27601 cacggaacgc gttgcaatga tgcccagtgc gtaagggatc tgaatcgcaa atttgttcgt
    27661 ctcctcttcc tgatcaggaa tgccgaacag agtaaaggca gcaggcgcag gttgcgtttc
    27721 ccactcggct tcaatagcag ccagtttggt tttctgcacg tcgcccattt cgtagccgga
    27781 ttcatcaccc agaacaataa cagacagaac agcagccata ccgaagctgg cagcgatagc
    27841 aaaggagcgt ttagcgaagg cgaagtcacg acctttcagc atataccatg cgctgatacc
    27901 gaggatgaac atcgcgccag tcacataacc agacgctaca gtgtgaacga atttcacctg
    27961 agcaaccggg ttaagcacca gctcggagaa gctcaccatc tccatacgca tagtttcaaa
    28021 gttgaaatcg gacgcgattg ggttttgcat ccagccgttc gcaaccagaa tccacagtgc
    28081 ggacaggttt gaaccgagcg ccaccagcca ggtgacacac atatgctgaa ctttacccag
    28141 acgatcccaa ccgaagaaga acagacctac aaaggtggat tcgaggaaga aggccatcag
    28201 accttcgatt gccagcggcg caccgaagat atcccctaca tagtgggaat agtaagacca
    28261 gttagtcccg aactggaact ccatggtcag accggtagcc acacccagag cgaagttgat
    28321 accaaacaac ttgccccaga acttggtcat atctttataa atctgtttgc cggagaggac
    28381 gtagaccgtt tccataatgg ccagcaggaa cgccataccg agcgtcagtg gcacaaaaag
    28441 gaagtggtac atcgcggtca aggcaaactg taagcgcgac agttcgacta tatctaacat
    28501 catgactcct tgctcatcgc atgaagactc cgagagtgaa ccccgttaga aagggtcaca
    28561 cgcatgcccc aatagaaatt tgtttgaaca ctttcatcat accatgtgaa tgcacacaaa
    28621 aacggcgtaa agaaaggttg caaacatgtt aataaaaact caaattgatc ccacgtatat
    28681 attacgccgc aaaatcctta caataaacag gtttttgttg aagcaaattt gcatttttcg
    28741 acggtgatca atttatagct aaattaccgc ctttcagcca atttgatcga gaacaattta
    28801 tctctttttg atgcccattt ccaagactta tacattgata aatatcaatt cctacaaatt
    28861 atgttaatca tgtttcaata aggtaacttt agtgacattt atgtttaaaa tgtgtgagtt
    28921 ataaggctga aattagtttc ggttggcaat taaagataaa aagagacgaa gcaaaaacag
    28981 cacggttgac cttaattctt tttttggtca ctggctaata cgacgcagag tttcaggatt
    29041 actaa
//
LOCUS       DAZGSP010000064        28629 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZGSP010000064 DAZGSP010000000
VERSION     DAZGSP010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.064"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(29..697)
                     /gene="artM"
                     /locus_tag="ACHK74_003042"
     CDS             complement(29..697)
                     /gene="artM"
                     /locus_tag="ACHK74_003042"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFP0221812.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(697..1413)
                     /gene="artQ"
                     /locus_tag="ACHK74_003043"
     CDS             complement(697..1413)
                     /gene="artQ"
                     /locus_tag="ACHK74_003043"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFP0221813.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1420..2151)
                     /gene="artI"
                     /locus_tag="ACHK74_003044"
     CDS             complement(1420..2151)
                     /gene="artI"
                     /locus_tag="ACHK74_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFP0221814.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2169..2897)
                     /gene="artP"
                     /locus_tag="ACHK74_003045"
     CDS             complement(2169..2897)
                     /gene="artP"
                     /locus_tag="ACHK74_003045"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFP0221815.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3115..3630)
                     /gene="ybjP"
                     /locus_tag="ACHK74_003046"
     CDS             complement(3115..3630)
                     /gene="ybjP"
                     /locus_tag="ACHK74_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0221816.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3756..4079
                     /gene="ybjQ"
                     /locus_tag="ACHK74_003047"
     CDS             3756..4079
                     /gene="ybjQ"
                     /locus_tag="ACHK74_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFP0221817.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4076..4906
                     /gene="amiD"
                     /locus_tag="ACHK74_003048"
     CDS             4076..4906
                     /gene="amiD"
                     /locus_tag="ACHK74_003048"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFP0221818.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(4903..5916)
                     /gene="ybjS"
                     /locus_tag="ACHK74_003049"
     CDS             complement(4903..5916)
                     /gene="ybjS"
                     /locus_tag="ACHK74_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP0221819.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6015..7445)
                     /gene="ybjT"
                     /locus_tag="ACHK74_003050"
     CDS             complement(6015..7445)
                     /gene="ybjT"
                     /locus_tag="ACHK74_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFP0221820.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQEHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(7456..8457)
                     /gene="ltaE"
                     /locus_tag="ACHK74_003051"
     CDS             complement(7456..8457)
                     /gene="ltaE"
                     /locus_tag="ACHK74_003051"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFP0221821.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(8494..10212)
                     /gene="poxB"
                     /locus_tag="ACHK74_003052"
     CDS             complement(8494..10212)
                     /gene="poxB"
                     /locus_tag="ACHK74_003052"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFP0221822.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(10345..11313)
                     /gene="hcr"
                     /locus_tag="ACHK74_003053"
     CDS             complement(10345..11313)
                     /gene="hcr"
                     /locus_tag="ACHK74_003053"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFP0221823.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(11325..12977)
                     /gene="hcp"
                     /locus_tag="ACHK74_003054"
                     /gene_synonym="priS"
     CDS             complement(11325..12977)
                     /gene="hcp"
                     /locus_tag="ACHK74_003054"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFP0221824.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(13121..14020)
                     /gene="lysO"
                     /locus_tag="ACHK74_003055"
     CDS             complement(13121..14020)
                     /gene="lysO"
                     /locus_tag="ACHK74_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFP0221825.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(14477..15172)
                     /gene="aqpZ"
                     /locus_tag="ACHK74_003056"
     CDS             complement(14477..15172)
                     /gene="aqpZ"
                     /locus_tag="ACHK74_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFP0221826.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            15598..17256
                     /gene="ybjD"
                     /locus_tag="ACHK74_003057"
     CDS             15598..17256
                     /gene="ybjD"
                     /locus_tag="ACHK74_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFP0221827.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(17253..18209)
                     /gene="ybjX"
                     /locus_tag="ACHK74_003058"
     CDS             complement(17253..18209)
                     /gene="ybjX"
                     /locus_tag="ACHK74_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFP0221828.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            18360..19475
                     /gene="macA"
                     /locus_tag="ACHK74_003059"
     CDS             18360..19475
                     /gene="macA"
                     /locus_tag="ACHK74_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFP0221829.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     DTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            19472..21418
                     /gene="macB"
                     /locus_tag="ACHK74_003060"
     CDS             19472..21418
                     /gene="macB"
                     /locus_tag="ACHK74_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFP0221830.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQGLLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAETEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(21491..21715)
                     /gene="cspD"
                     /locus_tag="ACHK74_003061"
     CDS             complement(21491..21715)
                     /gene="cspD"
                     /locus_tag="ACHK74_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFP0221831.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            21865..21939
                     /gene="yljB"
                     /locus_tag="ACHK74_003062"
     CDS             21865..21939
                     /gene="yljB"
                     /locus_tag="ACHK74_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFP0221832.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            22120..23358
                     /locus_tag="ACHK74_003063"
     CDS             22120..23358
                     /locus_tag="ACHK74_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0221833.1"
                     /translation="MSRAIHRLSDTLLRKLSGSPTTKNTFFNDGGNLSVRHSTNGLLT
                     WYFTYRAGTGRKVAPERLRLGNYPDLSLKAAREKASQCRAWLAEGKNPRHELNHTVQE
                     ALSPVTVKEALTYWLESYAKEKRTDYESLKSRINTHIISHIGAMPLEKCELRHWLTCF
                     DQLAMRNPVSAGFLLQVCKQALKYCRKRRYAITNVLDDMVVGDVGKKAEISERVLTNK
                     ELGELLRALDEKIYTPYYSALIRLLIVFGCRTTELRRSEVQEWDFKEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTKLVEQNRHTGLLLGELKGQSSVSEYGRTAHRRINQAPWTL
                     HDIRHTFTTMLNDLGVDPHVVEQLTAHQLPGMQRVYNHSRYLDAKRDALNLWVERLEL
                     LQNNDEKIVVMTPRIYSQNS"
     gene            23768..23977
                     /locus_tag="ACHK74_003064"
     CDS             23768..23977
                     /locus_tag="ACHK74_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0221834.1"
                     /translation="MREINEDRVIREDECRKLTGVCRTTRYELEKKGRFPSRLNLGGR
                     SVGWLLSEVMEWVKSRDRINSGKAV"
     gene            complement(24089..24265)
                     /locus_tag="ACHK74_003065"
     CDS             complement(24089..24265)
                     /locus_tag="ACHK74_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221835.1"
                     /translation="MTNEESTEKTSPARKRRRRKKEHESERFAPCSFALEKYLKEYSS
                     KERASQLWQRTETD"
     gene            complement(24323..24502)
                     /locus_tag="ACHK74_003066"
     CDS             complement(24323..24502)
                     /locus_tag="ACHK74_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0221836.1"
                     /translation="MATGSTNNKSQQLNARFPHDVVADLEKNLDEGESKAQFIVTAVK
                     GEIKRRQRRKAKESE"
     gene            24609..24896
                     /locus_tag="ACHK74_003067"
     CDS             24609..24896
                     /locus_tag="ACHK74_003067"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0221837.1"
                     /translation="MHQITTPRSGGTHAGRLTTNDSNSIEAAMRNYTRHPQGRDSHNL
                     NKYIWRFIALSTAQPRVITIEATSEQEARQQSPAGCVMVFAARIRQGSHHA"
     gene            24889..25353
                     /locus_tag="ACHK74_003068"
     CDS             24889..25353
                     /locus_tag="ACHK74_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0221838.1"
                     /translation="MRKNRLQKIITGLYASPVAHMATGFDSLNTTADSRLYSDMRFFC
                     VRKPTHTRIMAGRNGGAFALAGSCSASLSTLLRLATMFDSVVARLLNFTTGAFHMAVS
                     ARPYFVWRFMQCHHSSIDIYTVTAVTEREARAQLPHAHLIFVARIRQGVSHE"
     gene            25346..25573
                     /locus_tag="ACHK74_003069"
     CDS             25346..25573
                     /locus_tag="ACHK74_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221839.1"
                     /translation="MNKIPYDVLIHSENALIKAREMNALLIKLIDVHEGGGEPASLIF
                     AAVQTLLSPVIDELDKAMAIHENNNAPHTGE"
     gene            25579..25878
                     /locus_tag="ACHK74_003070"
     CDS             25579..25878
                     /locus_tag="ACHK74_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0221840.1"
                     /translation="MKLKYPGLTASGKTRTKFMCGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFDRDFSLVQAVPRNVPTSREKARANIQEIRKMLNVFRGKK"
     gene            25875..28007
                     /locus_tag="ACHK74_003071"
     CDS             25875..28007
                     /locus_tag="ACHK74_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFP0221841.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEHDARMA
                     GDNEPPVWLGDQQLSELDKLQIVPEGRKSARIFRAGHLEPVMIKAIGQKLAAAGVQDA
                     NFYHEGMHVKKVENWREYLARERQNLSDGLVIELPVKKKDTGSHSDDELKPRVESRAD
                     GVFWVTPKVDKQSGEIIRPETWLCSPLELLGTGTIGKEHYRVMRWKKTANHEVITMAI
                     PCGGIGDRDGWRLLKDHGLNVTTNGKYRAILADWMQLSGSHEEWQLSTTTGWHFGAYI
                     MPDGSIIGDSEKPILFTGKSAAINGYSVAGTAEGWRESVARLAGGNASMMLGVATSLA
                     APLIGLVGADGFGVHLFEQSSAGKTTTQNIASSLWGEPDAQRLTWYGTALGIANEAEA
                     HNDGLLPLDEIGQAGNAREVSTSAYTLFNGSGKLQGAKDGGNREIKHWRTVAISTGEM
                     DVETFLKSEGIKVKAGQLVRLLNVPMEKATKFHEYSNGKEHADALKDAWTANHGAAGR
                     EWVKWLAGHQQEAKDTVRECRERWRNLIPESYGEQVHRVGERFAILEAALVLSGHVTG
                     WVVQECRDAIQHNFNAWVKEFGTGNREFKQMVEQAEAFLSSFGFSRYLPYPNTDERDL
                     PIKELAGYRKGSIRNEDDEMRYYTFPHVFESEIAKGFNPAHFARALDAAGMLEKGSDR
                     RYKKKALGKIGGKQHVFYVLMFQPDDED"
     gene            28377..28628
                     /locus_tag="ACHK74_003072"
     CDS             28377..28628
                     /locus_tag="ACHK74_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221842.1"
                     /translation="MTQKRRDRTEPKYKALDMAELALKVAIRTIDRHAGEGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEGAV"
BASE COUNT         7187 a         7181 c         7592 g         6669 t
ORIGIN      
        1 acccccccgg cgaatgtacg cagctacatt aatttcgccg ttcgaatgcc agtgctttgc
       61 gctcgatcag acgcatcatc agcgtcagca ggccgttgac gaccaggtaa ataatccctg
      121 ccgcaccgaa caccattaca tcgtaggtgc gtccgtacaa caactggctg tatcccatca
      181 cttccatcag cgtaatggtg taagccagag aggtactttt gaacaccagc accacttcgt
      241 tggaataaga agagagcgag cgtttaaagg catacggcag caggatcgcc agcgtatctt
      301 ttttgctcat tcccagggcg ctacaggact gccactgacc ttctgggatc gcacgaattg
      361 caccgtaaaa cagctgcgtg gtatacgccg cactgttcag cgataacgca atcagcgcac
      421 ataaccacgg ttctgacaac aaatgccaca gtgccggata ctcctgcaaa gtcggaaact
      481 ggcccggccc gtaataaatc aggaagatct gcaccagcag cggcgtaccg gtaaacagcg
      541 tgatataacc ccgcaccagc cacaccagca ccggcgtttt cagcgtcagg atgatggtaa
      601 aaatcaatgc cagaatcagt gccacaatca gcgaggcaac ggttagcgtc aggctggtgt
      661 gtagcccttt catcagttcg ggtaaatact caaacattag ctgggcctcc gctcaaaacg
      721 tgtcgcgcgc aggtcaatgc gtttgagaat gtactgactg agcagggtga tcaccaggta
      781 aatcgccgcc gccacaatgt accaggtaaa tggttcctgg gtacgagtag cgatgctttt
      841 tgtttgcagc attaaatcat tcacactaat caaactgacc agcgcggtat ctttcagcag
      901 caccagccac tggttaccga ggccaggcag cgcatgacgc cacatctgcg gcatcaccag
      961 acggaaaaag atagccgatt tcgacagccc cagcgcctga ccagactccc actgacccac
     1021 cggcaccgct ttcaacgcgc cccgaagcgt ttgcgaggca taggcggcat acagcagtga
     1081 cagagcgatg acaccacaaa ggaacgggct aacgtcgaag ttctcaatgt ccatctgcac
     1141 tgggatctgc acgaacccaa gattgatagt gaagccatcc gaaagcgtca gcagcagctg
     1201 cgaggagcca aaatagataa acagcaccac cagaatttct ggcaggccac gcagaatggt
     1261 taccagcgct gaacctgccc acgcgacagg acgccatttt gccgactccc ataccgcaaa
     1321 gaacatcgcc agcgccagcc cgacaatcaa tgcacaaacg gcaaggccga cggtcatccc
     1381 ggcggcgctt gctaaaggaa aaaattcatt catcaggaat tacttctgga accatttgtt
     1441 gtagatggtt tcgtaagtgc catctttctt cactttttcc agcgcagtgt tgagtttctg
     1501 ctgcagctca gtgttgccct gacgtaccgc gatgccgagg ccagtgccga agtaatcttt
     1561 atcggtcact ttgtcgccca ccgccgccag tttcgggtta tctttcagcc actcagtgac
     1621 cactgcggtg tcaccgaaga cgccgtcgat acgcccgttt tgcagatcca gttttgcgtt
     1681 ctggtagctg tcatacggaa cggtagtgat ttccgggtgc ttatccataa tgaatttctg
     1741 gtgtgtcgtc ccgttctgaa cgccgacttt tttgcctttc agctgatcaa cactggtgta
     1801 tttgccttgc tgacccacaa acagggcaga gttgtcatag tacggggtgg taaacagtac
     1861 ctgcttttca cgctccggag tgatatccat gcccgccatc actgcttcta cgcgacggaa
     1921 tttcaggctt gggatcaggc tgtcaaacgc ctggttagag aaagtacagg ttgcatcaat
     1981 ctctttacac agtgcctgtg ccaggtcgac gtcaaaacca acgatctggt tgtttgcatc
     2041 aatcgattca aacggaggat aggaggcttc ggtagcaaaa cgaatggttt cggcagctgt
     2101 ggcggaaaga ctaaaacctg caattaacgc ggcaatcaga acttttttca ttgttgttat
     2161 cccgaatctt agtgagagag atagttttta aatgcttcgg tttgcggctc ggtaaagcag
     2221 ctcgcgtcgc cttgttctac gatatgacca ttttccatat acaccactcg gctggcggtt
     2281 ttacgcgcca cttcaacttc gtgggtgacg atcacctggg taatattcgt ttctgccagc
     2341 tcacgaatga tgctgacgat ttgtgccgta atttccgggt ccagtgcggc ggtcggttca
     2401 tcgaacagca gtacctgcgg ttccatcatc aacgcacggg caatagcaac acgctgctgc
     2461 tgaccaccag aaagatgcag cgggtaacga tcgctataag gtttgagacg cagacgttcc
     2521 agcagttttt ctgcacgggc cagcgcctga tctttactca accccagtac acggcagggc
     2581 gcttcaatca ggttttgctg cacggtcaga tgcggccaca ggttgtattg ctgaaacacc
     2641 atgccaacgt tacgacgcaa atcgcgaatc gctttgtcag agggtgtttt ggtgaaatcg
     2701 aaatggttgc ctgcaatgtt gagcgtaccg gagcgcggca tctcaagcag attgagtaca
     2761 cgcagcagcg agcttttacc cgcgccgctg gggccaagta acaccagcgt ttcgccctgt
     2821 gggcaatcca gcgtgatatc gaacagcgcc tgatgcgcgc cgtagaagca attaatgccg
     2881 tttaattgaa tactcattga cactcgtata ctggcagtct gatagctatt gaggtcgaag
     2941 atagtacctt tgacagaata attatgcaat atttctgctt taaaagttaa aagcaaagcg
     3001 cattattcaa taaacatagc acaaaataac gggggcggtg gtcggtgagc ataaatgtcg
     3061 gcattcctca cgaaatgccg gacaatttac ggggtttatt ggttgatcaa ggcgttagcg
     3121 attctcgatg gactgacgga gcgtacccgc tgtggcatga acgctgccgc ctaaatagcg
     3181 tacatcgtcg atcacccaac actggccttc ctgaatcatt aacacttcat cctgccagct
     3241 ctgatcaccc tgttttagat ccacacgcag aggaatattt cgcgcatcac gattcgggat
     3301 tgttgaggca ctggcaacac gggcgctctc tggcaaggtt gtgcgactgg agaatggatc
     3361 gttggtcagt agttcccgat ggttattatc ccgggaggca tcgctaagca gtgtcgccag
     3421 tttgtcgctc agataagggc gcaaggcggt gatgtcgttg ctgcggtgca aaatgcggta
     3481 gtcataaaat tgctgggcca cgttatccgg gcctccttca acgcaaggac cactgcgtgt
     3541 gccgttatct ttataagctg gagtgactgt ggtgcaggca ctgaggagca gtgcgcaggg
     3601 gataagcatt gtcaatttgc tgtagcgcat aatgatttcc ttataagcga tcgctctgaa
     3661 agcgttctac gataataatg atatcctttc aataatagcg tatcagtctg ataatgcttt
     3721 agagatcgaa ggcttaacaa acaaggagat cgatcatgca attttctaca accccaactc
     3781 tggaaggcca gaccatcgtt gaatattgcg gtgtggtgac cggcgaagcg attttaggtg
     3841 ccaatatttt ccgtgatttc tttgccggta tccgcgatat cgttggcgga cgttccggtg
     3901 cgtatgaaaa agaactgcgt aaagcacggg agatcgcctt tgaggaatta ggctcccagg
     3961 cgcgggcgct gggggccgat gccgtcgtcg gtattgatat cgactacgaa acggtcgggc
     4021 aaaacggcag tatgctgatg gttagcgtca gcggtacggc ggtgaaaacg cgtcgatgag
     4081 aagagtcttc tggctgatcg ctgccgctct gttactggca gggtgtgcag gcgaaaaagg
     4141 cattgtcgag aaagagggat atcagcttga tacccgacgc caggcgcagg cggcgtatcc
     4201 gcgcattaaa gtgctggtga tccactacac cgcagatgat tttgatagct cgctggcgac
     4261 actgaccgat aagcaggtca gctcgcatta tctggtccct gcggtaccac cgcgatacaa
     4321 cggtaaaccg cgcatctggc aactggtgcc ggaacaagaa ctggcctggc atgcggggat
     4381 tagcgcctgg cgcggggcaa cgcgccttaa cgacacctct attggcattg agctggaaaa
     4441 ccgtggctgg caaaaatcgg ccggagtgaa atattttgcc ccgtttgaac cggcacagat
     4501 tcaggcgctt attccactgg cgaaagatat tattgcccgt tatcacatca agccggaaaa
     4561 cgtagtggca catgcggata tcgcaccgca gcgcaaagac gatccggggc cattatttcc
     4621 ctggcagcaa ctggcgcagc aggggattgg tgcctggccg gatgcgcagc gggttaactt
     4681 ttaccttgcc gggcgcgcgc cgcacacacc ggtagatacc gcgtcattgc tggagctttt
     4741 ggcgcgctac ggttatgacg ttaaacctga tatgacaccg cgcgagcagc ggcgcgtgat
     4801 tatggcattc cagatgcatt tccgcccgac gttatataac ggcgaagcgg atgcagaaac
     4861 tcaggcgatt gccgaagcat tgctggagaa atacgggcag gattagcgcg gcagttttcc
     4921 gtggtcgcgt agccaggcgg cagttttctc gataccttca tccagggtga tgaccggctg
     4981 ataacctaac tcttcctgcg cacgcgtaat atccagcgta aagtcaaaat tcaacttgga
     5041 gacgccgtag tgggtcagcg gcggctcttt tgctgacttg cggcctaaac gctccatgct
     5101 gcgggcgatc atatccagca tcgggtaggg gacggaacga atacgacagt caatattcaa
     5161 ctcgtcgatc agcttctgca cgatgctgcg cagtgtgcga tgctcgccgt tggtgatgtt
     5221 gtacacacgc ccggaaggta gcttatcgca ggcttcctgg cttgccagcc acattgcgtg
     5281 cacggcattt tcatagtagg tcatatcgac cagcgcactg ccgccatgcg gtaacagaat
     5341 actgccgtag tggtgcatca tatgcgccag acggggaata aagactttat cgtgcggtcc
     5401 gaacagactt tgtgggcgca gaatagtaaa gcgcgtttgt ggattcgcct gcgaaagcat
     5461 attgatcact tcttcgctgg ctgctttgct gcgggcaaac tcgttggcga agcggtgagg
     5521 gcgaaaatct tctttaatat cgcgatggtg gtgataatcg aagtacaggg agggggaaga
     5581 gatatgaata aagttacgca caccccaggc gacagcccat tcacccaggc ggcgagtggc
     5641 gcgaacgtta gccagatcga aagcctgttg tgtcccccag ggtgaggtaa agctggagca
     5701 gtgccacagc gtatcaatgc ccgcgagcat cactttagct tgtgatgaaa ccagctcggt
     5761 cagatccgcc ggaacaaact ctgcgcccat tttttccagc aatttgccca ttgcctcgtt
     5821 gcgaccggtc gctcgcacgc tgatgccttt ctggcataaa aactctaccg cgtttcgacc
     5881 taagccgctg gtggcgccgg taaccagtac cttcatatca atccactgtt gttgagaaaa
     5941 taacgtgcgc attcttccgt gatttccccc atgatgcaat gggaaacatg aaagaataac
     6001 gcaggttttg tcgattaatc tgtgctttgt tctgccagtc tggcgatttg ttttgccatt
     6061 ccgcgaaaaa taaacagatg cgcggggatc atcaataacc agtaaaacag ccccggcata
     6121 ccgtgcggat gccagaaagc gcggacatcg atagtacgat agtcgccttt atcttccagg
     6181 ctaaaacaca gtcgtcccag ccccggcgct ttcatgccaa ataacaacgt aagttgtttt
     6241 tccggttcaa cgacaatcac tttccagcta tctaccgcat cgccagtctg taaatattcg
     6301 cgctccgggc ggcctttcgc cagcttatga ccgatcgcgc ggtccatcaa cgcccgtgtc
     6361 tgccacaaaa tattgccaaa gaaataacgc tctttaccgc cgatttggtt cactacctgc
     6421 cataaagcag caaggctggc ggacgtttta acggtaaacc ccgcctgttt ggcaaaataa
     6481 ccgtactccg gtcgccagcg ggcaaaggcc tgagcgtcgt agccccagtc gctggagttg
     6541 accagttttt cctcctcttt caacgtgcta cgtaccgcgt catcgaaagc gatcagccgt
     6601 tgtgggatga gtgcacgtag cgcggtatca tccgccagca gatcgtgttt cagcccctga
     6661 atcaacgccc tggcggtggt gggcggtacg gaagtaatca cattgagaaa ccacaccgaa
     6721 atccagcggg tggggagggg gatggggatc aaccagcggc gcttaccgct caccgccata
     6781 aaatgttcaa actgttgctg ataactgagc acctctggtc cggcggcttc gaagatgcgg
     6841 tgttcgctgg ccggatgatc taacaacgcc accagatagt gcagcaagtt ttccagcgcg
     6901 atgggcgtgg tgcgtgaacg tacccagcgt ggcggcgtta acactggcag gttgtagacc
     6961 atatcgcgca tgacttcgaa cgccgctgaa cctgcgccaa cgataattcc ggcccgaagt
     7021 tcggtcacag gtacattcgc ttcacgaaga atgtccgccg tagcctgacg agcacgcaga
     7081 tgatccgact gctcatgtgg cggggcctgc aacgaactga gaaagattaa ttgcttaact
     7141 ggtacttcac gtagcgcatc gcggacgttg agagccacct ggcgctcctg agcgataaaa
     7201 tcgccgcctt cgcccatgct gtgcaccaga aaatagacgg tatcgatatc ctgcaacagg
     7261 gccggaaggt tatccggcca gctgagatcg actttatggc aactgacgtt tgccagttgc
     7321 agctttgcaa gcctgtcgac atgacgtgcc gccgccagga tctgatgctc ttgctggctg
     7381 agtgtgcgca ccagatgctg accaatgtag ccactggcac cgagaactaa aatgcgttgc
     7441 ggcacgtctc tctccttaac gcgccaggaa tgcgcgccag tgggcggcga cttccgccag
     7501 ttgttcgcgc gagacgtcaa gatgcgtcac caggcggaca atcggcgagg cgttaatcag
     7561 cacgtttctc gctttcatgt attcgcctaa cgcggcagca ttttcttccc cgacgcgaac
     7621 aaacagcata ttagtgtcct gacgcatcac atccgcgcct gcttcacgca gttgctccgc
     7681 catccaggcg gcgttgtcgt gatcttcctg caaccgtgcg acgttatttt tcagggcata
     7741 catcccggcg gcagccagaa tcccggactg acgcatcccg ccaccggcca ttttccgcca
     7801 gcggatggca cgtttaatgt aatcacgatt accgacgagt aatgaaccga ctggcgtccc
     7861 aagacctttc gacaggcaaa tggtgaacga atcacaatat tgcgtgatct ctttcagttc
     7921 gcagccgtaa gccaccacgg cattaaagat gcgcgcaccg tcaacatgca gcgccagatt
     7981 gcgctcgcgg gtaaattccc atgcttcttt caggtattca cgcggcagca ctttgccgtt
     8041 gtgggtgttt tccagactga gtaatttggt gcgggcgaaa tggatatcgt cgggtttgat
     8101 tttcatcgcc actttatcca gcggtagcgt gccgtcggca gccgcgtcga tgggttgcgg
     8161 ctgaatactg cccagcaccg ctgcgccacc ggcttcaaac agatagttat gcgcggcctg
     8221 accgacaata tactcttcgc cacgttcgca gtgactgagc agagcgacca ggttggcctg
     8281 agtgccggta ggcagaaaaa tggcggcttc tttaccggaa agctctgctg catagtcctg
     8341 cagagcatta acggtagggt cgtctccgta aacgtcgtcc ccaaccgggg cggccatcat
     8401 cgcttcgagc atggcgcggc tcggtcgggt aacggtatca ctgcgtaaat caatcatggc
     8461 atgtccttat tatgacggga aatgccaccc tttttacctt agccagttcg ttttcgccag
     8521 ttcgatcact tcatcgccgc gtccgctgat aattgcgcgc aacatataca ggctaaaacc
     8581 tttggcctgt tcgagtttga tctgcggtgg aatggctaac tcttctttgg cgaccaccac
     8641 atccaccaac accggaccgt cgatagagaa ggcgcgttgc agggcttcat caacttcaga
     8701 cgctttttct acacggatac ccgtaatgcc gcacgcttcg gcaatgcggg caaagtttgt
     8761 gtcgtgtagt tcggtgccgt cagtcaaata gccaccagct ttcatctcca tcgccacaaa
     8821 gcccagcacg ctgttgttaa agacgacaat tttcactggc agtttcatct gcactactga
     8881 gaggaaatcg cccatcaaca tgctaaaacc gccatcgccg cacatggcga ccacctgacg
     8941 ttctggctct gtcgcctgcg cacccagcgc ctgcggcatg gcgttagcca tcgaaccgtg
     9001 gttaaacgaa cctaacaggc gacgcttgcc gttcattttt agataacgtg ccgcccacac
     9061 cgttggcgta ccaacgtcac aggtgaaaat agcgtcatcg gcggcaaaat gactaatttg
     9121 ctgcgccaga tattgcgggt gaatggcttt ctcgctcggt ttagctaaat cgtccagccc
     9181 tttgcgggcg tcgcggtaat cttccagcgc tttatccaga aacttgcgat cggctttttc
     9241 ttccaccaat ggaagcaatg cacgcagagt cgacttgata tcgccgacca gtgccatatc
     9301 caccttgctg tgagcgccga tgctggctgg gttgatatca atctgaatga ttttggcatc
     9361 ggtcgggtag aaggcgcggt agggaaattg cgtgccgagt agcactaacg tgtcggcgtt
     9421 catcatggta tggaaacctg acgagaagcc gattaacccg gtcattccaa catcatacgg
     9481 attatcgtat tcgacatgtt ctttaccgcg cagggcatga acaataggcg ctttaatttt
     9541 cccggcaaac tcaactaact ctttatgcgc ccccgcgcag ccgctgccac acatcagggc
     9601 gatattgctg gaataacgca gcagttgcgc cagtttgcgt aactcttctt cttccggcgt
     9661 cacgactggt tgtggcgcat gataccagtg cgtggttgcc ccttctggcg caggttttaa
     9721 cgccacgtcg cctggtaaca cgacaaccga aacgccacgg ttaagcaccg ctttgcgcat
     9781 ggcaatcgcc agtacttgtg ggatctgctc cgggctggaa accaactcgc aatagtgact
     9841 acattcgcgg aatagctctt gtgggtgggt ttcctggaaa tagccgctgc caatttcgct
     9901 ggagggaata tgagcggcaa tcgccagtac cggaacgtga ttgcggtggc aatcgaacag
     9961 gccgttgatt aagtgcaggt tgccggggcc gcacgatccg gcgcagaccg ccagttctcc
    10021 gctaagttgt gcttcagcgc cagcggcaaa ggccgccact tcttcgtggc gggtggacat
    10081 ccactcgatg gtgcccatgc gattaagact gtcactaaga ccgttcagag agtcgccagt
    10141 gactccccag atgcgtttca cccctgccga ttcgagtgtt ttggcgatat aagctgcaac
    10201 cgtttgtttc atggttctcc atctcctgaa tgtgataacg gtaacaagtt tagttcatct
    10261 gacggagggg gaagggatgg gagagaaagg aggcactaac ggttaaatag cccgatgaaa
    10321 ggaatatcat cgggcataag gcgattatgc gagaaccaaa tccccctgcg gatggcagga
    10381 gcaggccagt acgtaacctt cagcgatttc ggcgtcggtc agcgtcattg tactgctcac
    10441 cgtatattca ccggaaacca cttttgtctt acagcagccg caaacacccg cacggcaggc
    10501 agcgacaacc ggaacgttat tgctttccag cgcctccagt agtgtggtgc caaccggggc
    10561 gtaaaattct cgtgccggtt gcagtttggt gaatttcaga ccgctggtcg ccgcttctgc
    10621 tactggggtg aagaatttct ctttaaagaa acgcgtcacg ccgagcgctt tcacttcctg
    10681 ctctacccaa tccatatacg gagccgggcc gcaggtcatc acggtacgtg aagctaagtc
    10741 aggtacacct gccagcagtt cgcgagtgag acgaccagcg ataaagcctt cggtaacgtt
    10801 attttctgcc accagcgtta ccggatagtt acgccactca tcggcgaaaa taacatcctg
    10861 cggcgtacgc acgttgtaga tcacccgcac atcggcctgt ggacggttct tcgcaagcca
    10921 gcgacgcatc gacataatcg gcgtgacgcc gcagcctgcc gccagcaaca ggaatttatc
    10981 ttctgcttta tcgtcgcagg taaattcccc catcgcgtcc gaaagccaga gataatcacc
    11041 gcgttttaca tcgcgcgtca gccactggga gccgacaccg tcatcaatcc gccgcacggt
    11101 cagggtgata tattcactca cgcctggcgt ggaggaaatg gtgtaagcac gcagcgtttc
    11161 cgctgagttt cgcacgctga ccagtgcata ttgcccggcg cgatatgggt agtaatcgtg
    11221 gcaaatcagg gaaatcgtcc acacatccgg cgtttcttgc gtaatgtgat gaacctgcat
    11281 ccgccacggg cattgattcg ttggcatcgt catcgacaaa ctccttacgc gctcaacagt
    11341 tgcttcatgt cttcttcaac agtggtgata gaacgcaggc caaatttctc gttcagcacc
    11401 gccagcaggt ctggtgtcag gaaaccaggt gcagtcgggc cggtgacgat atttttcacg
    11461 cccagagaaa gcagcgtcag cagaatgacg atcgctttct gttcaaacca ggagagcacc
    11521 agcgacagcg gcagatcgtt gacaccgcag cccagtttct ctgccagagt gacagccaga
    11581 ataatcgctg agtaagcatc gttacattga cctgcatcta ccagacgcgg cagaccttcg
    11641 atatcgccaa actcaagttt gttaaagcga tatttaccac aggcgagggt caggatcagg
    11701 cagtcatccg gcacgctggt ggcgaaatcg gtgaagtagt ggcgctcgcc gcgtgcgccg
    11761 tcacagccac caagcaggaa gatatgacgc agtttttcac ggctcaccag atcaatcagc
    11821 gtatcagcag cgccaagcag cgtctggcga ccaaaaccca cggtgatcag gtgcggaatt
    11881 tcgctgtacg ggaagcccgc catttgttgc gcctgggcga tgaccgcaga aaaatcttcg
    11941 ccatccagat gacgcacacc cggccagcca acgatgcttc gggtccagat acgatcgtca
    12001 taagcgccta cggttgggtc gatgatgcag ttcgaggtca tcacgatggg gccagggaaa
    12061 cgagcgaact ccacttgctg attctgccag ccactgccgt agttaccgac cagatgcttg
    12121 aatttacgca gctccgggta gccatgcgca ggcagcattt cgccgtgggt gtagacatta
    12181 acgcccgtgc cttcggtctg ttccagcagg ttgtagagat ctttgagatc gtgaccggaa
    12241 atcagaatgc atttacccgc cgtcgctttg acgttgacct gggttggcgt cgggtgaccg
    12301 tatttaccgg tttcgcctgc atccagaatg ctcatcactt tgaagttcat ctggccgatt
    12361 tccattgaac actcaagaag cgcgttcata tcggcaggcc aggtccccag ccacgccatg
    12421 attttatggt actgggcata aatatcgttg tcgtattgac cgagaacatg cgcgtgttcc
    12481 atataggccg ccgcaccttt caggccatac aggcacagca gacgcaggcc gagaatgttt
    12541 tcgccaatcg ccgctttatc tttgttaggg gtaaattctg ctgcctgacg ttgcagctcg
    12601 ccgagatcgt cgctcaccag ttgcaggtca gccatcgggt tatcgacgcg cgcgttggca
    12661 tctacagcca ggcattgcgc tttcagcgcc tcgcgcaggg caatcgcttc acgagcgtag
    12721 ccgacaatac gcggagaatc gaagttaacg ttggtcaggg ttgagaaaaa ggcacgtggc
    12781 gcgaagctgt caacatcgtg gttgatgatg ccgtattcac gcgcttttac cgcccaggca
    12841 gaaagccctt gcagcgccgc gatgagtaaa tcctgaaggt cagaagtttc cgccgtttta
    12901 ccacacatcc cctgcgcgta tgagcagccg tttcctgccg gagtacggat agtttgttca
    12961 cattgcacac aaaacatgat cacacctttt aaagttatat ttaatataca tgtttaaggt
    13021 taagacgctt aacgcgggga taaaagggat ttttcatgca actttaaggg agattgattt
    13081 agcgcaattt tggcggcagg gatctaccgc cagagaggta ttacgcagag aaaaaggcga
    13141 tgaggatcgg cactaacagg ctaagaataa aaccgtgaac aattgccgcc gggaccatat
    13201 ccagcccgcc agtacgttga agaacgggca gggtgaaatc cattgatgtg gcaccgcata
    13261 agcccagtgc agtagagcgg ctgcggcgaa tcagcccagg gatcaacata atagcaatca
    13321 gttcacgggc cagatcatta aaaaacgccg cgctcccgat taccggacca aaagattcgg
    13381 tcaataaaat accggaaaga gaataccagc cgaaaccgga ggccattgcc agcgcggtat
    13441 tgatggggag atcaagaata aaggcgttaa ttaaaccacc aattaatgaa ctgacaacca
    13501 ccaccacggc gacaatcatt ccccggcgat taaggacaat ctgctttaag gtcatgccat
    13561 tattgcgcaa ctgaatacca acgaggaaaa gtagcaaaat taacgtgtat tcactggctt
    13621 cggtcgcgtg ttgtaagaaa gccagtccac tcagaccaat ggcaaaacca atcactacta
    13681 cgccgcacag ttttagcgac tccagcgcca tcgcaatacg cgacgggagt ttttcttgct
    13741 gatggtggtt gcgccacggc aggcctcgct ccagccacat cagggcggca atattacaca
    13801 gtaaaataac ggtaatactg acggcagaat aatgcagaat cgccaacagg ttactggcga
    13861 ggttatcgag aaacgccaga cttataccca taaaaaagag aataaggtaa accatccagc
    13921 ttaatagctg attaataact tttaacgcag cttgttggcg aagcggaatg aggtaaccca
    13981 caatcagggg aaccagaatg attaacagcc cagaaaacat gaaaacccag tccttgcaaa
    14041 gatgaagtcg aaatgcgcga tgacacacta ctgaaagcgg aaggacgagt aaagttgcaa
    14101 ttaaaaggaa atgttatgca tcagaggggg atttttaatc ctatctgagt gtttagcatt
    14161 gttgcgctcc tttcatgtgc aatgccatat aaagccactg gcgcagggag cgcgcagggg
    14221 gcggccagtc gccgccgccc cctgcacccc cgggctctgg cgaacaaaat cgccgctgcg
    14281 cggtaccttc agcttatccc cttcggctac cgggtcgggc gcgaggtaac gtcctgtaag
    14341 acgcgccctc agcccacatc catgtgggct gccccggcct tcggggaacg cttcagcgat
    14401 tttgacgcca ccaacacccg tgcggtttgt aacgtcgaag ataaaacaac cagggcagat
    14461 aaaatgccaa tgctaattaa ttacgttttt ccagcagggt ccggtaaatc agaccaccga
    14521 taatgccgcc gacaattggc accacccaga agaaccacag ttgttctaat gcccagccgc
    14581 cctggaagat agcaaccgcg gtgctgcgcg ccgggttaac agaagtgtta gtcaccggaa
    14641 tactaattaa gtgaatcagg gttaaggcca gaccaatagc gatcggcgca aaacctgccg
    14701 gcgcgaattt gtcggttgcg ccgtggatca ccaacaggaa acctgcactc aataccagtt
    14761 caactaccag cgcggaaagc atggaataac cgcctggtga atgctcgcca taaccgttag
    14821 aagcaaaacc gctggctgcc gcgtcaaaac ccgttttacc actggcaatt aaatacagca
    14881 gcgccgctgc aacaataccg ccgacaacct gggcaattac gtagccaacg acttcttttg
    14941 ccggaaaacg tccgccagcc cataaaccaa tagtgaccgc cgggttaaaa tgaccaccag
    15001 aaatatgacc aacagcaaag gccatcgtca gaacggtcag accgaacgcc aacgccacgc
    15061 cggcaaaacc aatgcctaat tccgggaagc ctgcggccag tacagcacta ccacagccac
    15121 caaaaacaag ccagaaagta ccaaaacatt cagctgctaa ttttctgaac atatccacct
    15181 caattaaaaa ttgaccctgt gaaaaatatg gtcgttttat agggtcgtcg taaaaaagtg
    15241 acgacggaaa taatgcgcgg ctattttaaa aacgaaggcg agtcattcac cagataaata
    15301 aatccagtaa atttgattta gggcaacagc gggttgcccc atatagtcat ttgtctgatt
    15361 gacagtgtag tgcaagcaaa agatttaatc ctttaggcgt aataaaaaat aatttatcat
    15421 gctaattatt tgattttgtt gtttttgcag acttatcagc aagagggagt ataacgcgat
    15481 tattcgctca tttttcagac atttgccatg cttaaatgtg atgtcatcac gtattagcaa
    15541 ggcctttccc gttatactgc cagcgtaaag gataagtcac atatttctgg aggggatatg
    15601 attcttgagc gcgttgaaat tgtgggtttt cgcggtatca accgtttgtc gttgatgctg
    15661 gaacaaaaca acgtcctgat tggggagaac gcgtggggta aatccagctt gctggacgcc
    15721 ttaaccctgc tgctatcgcc agaatcagat ctctaccatt ttgagcgcga cgatttctgg
    15781 ttcccgccgg gagatatcaa cgggcgagaa catcatctgc atattatttt gaccttccgc
    15841 gaatcgctgc caggccgaca tcgggttcgc cgttatcggc cgctggaagc gtgctggacg
    15901 ccatgcaccg atggctatca ccgtattttt tatcgtctgg aaggggagag tgcggaagac
    15961 ggcagcgtga tgacactgcg cagttttctc gataaagacg gacatccgat tgatgtcgag
    16021 gatattaacg atcaggcacg ccatctggtg cgtttaatgc cggtgctgcg cttgcgtgat
    16081 gcccgtttta tgcgccgtat tcgtaacggc acggtgccaa atgtccccaa tgtggaagtc
    16141 accgcgcgcc agctcgattt cctcgcccgt gagttgtcct cacatccgca aaatctctct
    16201 gatggacaga ttcgtcaggg actttccgca atggtgcagt tgcttgagca ttatttctct
    16261 gagcaggggg ctggacaggc gcgatatcgt ttaatgcggc ggcgggccag caatgagcaa
    16321 cgaagctggc gctatctgga tatcatcaac cggatgattg accgacctgg agggcgctcg
    16381 tatcgggtta ttttgctcgg cctgtttgca actttattgc aggcaaaagg cacattgcga
    16441 ctggataaag acgcccgtcc attgttgctg attgaagatc cagaaactcg tttacacccc
    16501 attatgcttt cagttgcctg gcatctgttg aatcttctgc cattgcagcg cattgccacc
    16561 accaactcgg gtgagttgct ttcgttaact ccagtggaac atgtttgccg cctggtgcgt
    16621 gaatcttctc gtgttgccgc ctggcgtctg gggccaagtg gcttgagcac cgaagacagc
    16681 cgccgcatct cttttcacat tcgttttaat cgtccgtcat cgctgtttgc tcgctgctgg
    16741 ttgctggtgg aaggggaaac agaaacctgg gttatcaacg aactggcgcg ccagtgtgga
    16801 caccatttcg atgccgaagg gatcaaggtc attgagtttg cccagtccgg gctaaagcca
    16861 ctggttaaat ttgcccgccg aatggggatt gaatggcatg tactggtcga tggcgatgaa
    16921 gcagggaaga aatatgccgc tacggtacgc agcctgttga ataacgatcg ggaagccgaa
    16981 cgagaacatt taacggcgtt accggcgctg gatatggaac attttatgta tcgccaggga
    17041 ttttccgatg tgttccaccg cgtggcgcaa atcccggaaa atgtaccgat gaatctgcgc
    17101 aaaattatct cgaaagcgat ccatcgctct tccaaacccg atcttgccat tgaagtggca
    17161 atggaggccg gacgtcgtgg tgtggactcc gtaccgacgc tgctgaaaaa aatgttctca
    17221 cgcgtgctgt ggctggcgcg cggtcgcgcg gattaaccgc gaaacatcgt ggccatttgt
    17281 ggctgaatag cgtcgagcat ctcatagcgc cgacggtatt cagcccgttt tttactggcg
    17341 atttcggcaa tctcttttcg tgctatctgt gctggaaggc ggtaatggcg ttcagcatca
    17401 catacgccgc caaccgattc ccagaaagcg ttgtaatcgg catggatctt gccttcttta
    17461 tcacgataac gcaggctgcg gtaaatatgc gtttcattgc tgacggcaat aatctgctct
    17521 acctgcaaac gttgggcaaa cagacaggcc gcttccatca cgaggcgttt gggaaatagc
    17581 ccgtggcagg ctttcgtcgc attctggatt tcctgatgtg gaatttccca ttttgcgcct
    17641 tgcagtccgc caataaacat cgttcttttc ccctgatatt cacacagggt aaacgtgatc
    17701 tctgccagag gaataccttc gctgttgcga aacaggattg tgctgtcacc ttctttatcc
    17761 attgagatca tcatggtcag ctcaagcgtg aactgctcgc cgtttttgcc ttccagcttc
    17821 gccagttgca gcccgggggt attcaaatat aagctgaatt cttccgccga catacatccg
    17881 cggagtaacg cataatggta acgtaacgcc tccagcaatt gcttacggct aagattcgcc
    17941 gcaaggtaag ggcgatgcag acgcacaggc agtcgcggtt ggcgcgttaa caacacattg
    18001 agattaggcc agtgggaaag ttcgttcatc cactcaacgc ttaaacgcgg cataatcaac
    18061 gagcgcagca aaaatttctg gcgaaaacta cggcgatgcc agaatttacc cggccgacac
    18121 tgtccacgtg ccagactaag aaaaagtgac aggctgctga gagattcaga tggcgtaaag
    18181 gtccgttcag ttagctgcga catattcatg aaatcaatgg ttatacatga cgtcgatttc
    18241 accattgcgt atcttaacca aacatcaata gtgtgattac taacgtaaat tttagggttt
    18301 tgttgatatt tcgttgaagt taatgacccg gattggcata tggagtattc agaatattta
    18361 tgaaaaagcg gaaaaccgtg aagaagcgtt acgttattgc gctggtgata gtcatcgccg
    18421 gactgattac gttatggaga attcttaacg cacccgtgcc gacttatcag acactgattg
    18481 tgcgccccgg tgatttacag caaagcgtgc tggcgaccgg aaagctggac gcgctgcgta
    18541 aggttgacgt gggcgcgcag gtcagcggtc agttgaaaac tctgtcggtg gcgattggcg
    18601 ataaagtaaa aaaagaccag cttttagggg ttattgatcc tgaacaggct gaaaaccaga
    18661 ttaaggaggt cgaagcaacg ctgatggagc tacgtgcgca gcggcagcag gcggaagcgg
    18721 agctgaaact ggcgcgggtg acgtattccc gtcagcaacg tctggcacaa acgcaggcgg
    18781 tttcacagca ggatctcgac accgccgcga cggagatggc tgtgaaacag gcgcaaattg
    18841 acaccattga cgcgcaaatc aagcgcaatc aggcttctct cgatacggct aaaaccaatc
    18901 tcgattacac tcgcatcgtt gccccgatgg ccggggaagt cacgcaaatc accactctgc
    18961 aaggccagac ggtgattgcc gcacaacaag caccgaacat tctgacgctg gcagatatga
    19021 gcaccatgct ggtaaaagcg caggtttctg aagcggatgt aatccacctg aagccggggc
    19081 aaaaagcctg gtttacggtg cttggcgatc cactgacgcg ctacgagggg caaatcaagg
    19141 atgtactacc gacgccggaa aaggttaacg acgctatttt ctattacgcc cgttttgaag
    19201 tccccaaccc caatggtttg ctgcggctgg atatgactgc gcaagtgcat attcagctca
    19261 ccgatgtgaa aaatgtgctg acgatccctc tgtcggcgtt aggcgatccg gttggcgata
    19321 atcgttataa agtcaaattg ttgcgtaatg gtgaaacacg cgagcgtgaa gtgacgattg
    19381 gcgcacgtaa cgataccgat gttgagattg tcaaagggct tgaagcgggc gatgaagtgg
    19441 tgattggtga ggccaaacca ggagctgcac aatgacgcct ttgctcgaat taaaggatat
    19501 tcgtcgcagc tatcctgccg gtgatgagca ggttgaggtg ctgaagggca tcaccctcga
    19561 tatttatgcc ggtgagatgg tagcgattgt tggcgcttca ggttccggta aatcgaccct
    19621 gatgaatatt ctcggctgtc tggataaggc caccagcggc acctatcgcg tcgccggtca
    19681 ggatgttgcc acactggacg ccgatgcgct ggcgcaactg cgccgcgagc atttcggctt
    19741 tattttccag cgttaccatt tactttcgca tttaaccgcc gagcagaacg ttgaagtacc
    19801 cgccgtctat gccggtcttg agcggaaaca gcggctgctt cgcgcccagg ggttactgca
    19861 acgactgggg ctggaagacc gtacagagta ttatccggca cagctttcgg gtggtcagca
    19921 acagcgcgtc agcatcgcgc gggcattgat gaacggcggt caggtaattc ttgccgatga
    19981 accaaccggc gcactggaca gccattctgg cgaagaggtg atggcgatcc tgcatcagct
    20041 gcgcgatcgc gggcatacgg tgattatcgt cacccacgat ccgcaggtcg ctgctcaggc
    20101 cgagcgggtg atcgaaattc gcgacggcga aattgtgcgc aatcctcccg ccattgaaaa
    20161 agtgaatgtt gctggcggga cggagcctgt tgtcaacacg gtgtctggct ggcggcagtt
    20221 tgtcagcggt tttaacgagg cgctgacgat ggcatggcgg gcgctggcag cgaataaaat
    20281 gcgtacttta ctgaccatgc tggggattat tatcggtatt gcgtcggtgg tttccattgt
    20341 cgtggtgggt gacgccgcca aacaaatggt gctggcggat attcgttcta ttggtacgaa
    20401 tactattgat gtctatcccg ggaaagattt tggcgatgac gatccgcaat atcagcaggc
    20461 gctgaagtac gacgacttaa tcgccatcca aaaacaaccg tgggtcgcct cagccacacc
    20521 tgccgtctcg caaaacctgc gcctgcgtta taacaatgtt gatgttgctg ccagtgccaa
    20581 tggcgtgagc ggcgattatt ttaatgtcta tggcatgacc ttcagtgaag gaaacacctt
    20641 taatcaggag cagctgaacg gtcgtgcgca ggtcgtggtt ctcgacagta atactcgccg
    20701 ccagcttttc ccccataaag cagatgtggt tggcgaggtg attctggtcg gcaatatgcc
    20761 cgccagagtc attggtgtgg cggaagaaaa acagtcgatg tttggtagca gtaaagtgct
    20821 gcgtgtctgg ctaccttaca gcacgatgtc cgggcgagtt atgggccagt cgtggctgaa
    20881 ctccattact gtcagggtaa aagaaggatt tgacagcgcc gagacggaac agcaactcac
    20941 gcgtttactt tcactgcgcc acggaaagaa ggatttcttt acctggaaca tggacggcgt
    21001 cttgaaaact gttgaaaaga ccacacgtac tttacaactg tttctgacgc tggtggcggt
    21061 gatttcgctg gtggtgggcg gtattggtgt aatgaatatt atgctggtgt cagtgaccga
    21121 gcggacgcgg gaaattggca ttcgcatggc tgtaggtgcg cgagcaagcg atgttttgca
    21181 acagttcctg atcgaagccg tactggtttg cctggtcggt ggcgcgttgg gaataactct
    21241 gtcactgtta attgctttca ccttgcagct tttcttaccc ggctgggaga ttggtttttc
    21301 accgttggca ctgctgctgg cgtttctctg ctcgacggcc accgggattt tatttggatg
    21361 gttacccgca cgaaatgcgg cacgactgga tccagtagat gctctggcac gagagtaatt
    21421 tttgagataa aaatgccagc cgatcgggct ggcattttgc ctttaggatg tacacaatga
    21481 gacagaagag ctatgcgact gccgcttcta cttcgacggg cacaataaca ctggcgtgat
    21541 tgccttttgg cccctggtgg acatcaaact gaacggattg tccagctttt agcgttctgt
    21601 aaccatccat ctgaatggtg gaataatgag cgaaaatatc ttcgccgccg ccttcagggc
    21661 agatgaaacc aaaccctttg gcattgttga accacttaac agtacccttt tccatgcttc
    21721 gacatccttc gcaaatctta tacaagtaag atggaataaa ccggggtcag agagggggct
    21781 gttcaaaacc tcgccaactc tagaaataca atttagagaa ttagggcgag ccgtcaagcg
    21841 tttgacaggg gccagggggc aggtatgaat caaaaatttg aagcagttaa cgctattgac
    21901 aggaatgtga cagatgtcgc tgatgccaac gatagatgat agttatttat cagttagtgt
    21961 tcgtgggtgt tcgtcatggt gtaggtatct catttatttc tatgattata aaggataatt
    22021 tcgtttttct tgttcgccct tgttcgtcac agttcgccct tgtacgatgc tcggtgtagt
    22081 caatggtgta gttaattttg atgaccggaa attcgaaaca tgagcagggc aattcataga
    22141 cttagcgaca ctcttttacg aaaattaagc ggatcaccaa ccacaaaaaa cacctttttt
    22201 aatgatggtg gcaaccttag cgtaaggcat tcaaccaatg gcctgttaac ctggtatttc
    22261 acttacaggg ccggaacagg caggaaggta gcccctgaac gtctgaggct gggaaattat
    22321 ccagatttga gcctgaaagc agccagggaa aaagcgtcgc agtgtcgcgc ctggcttgct
    22381 gagggtaaaa atccacgcca tgagcttaat cacaccgtac aggaagcgtt atcacctgtt
    22441 acggttaagg aagcgctcac ttactggctt gaatcgtacg cgaaggaaaa gcgcacagat
    22501 tacgaatctc tgaaaagccg gatcaataca cacataatca gccatattgg tgctatgccg
    22561 ctggaaaaat gcgagctacg ccactggctg acctgttttg atcagcttgc tatgcgtaat
    22621 ccggtatcag caggattttt gttacaggta tgcaagcagg cgcttaagta ctgccgcaaa
    22681 cgacgctacg caattaccaa cgttctagat gatatggttg taggtgatgt tgggaaaaaa
    22741 gcagaaataa gcgagcgcgt actaacaaac aaggagcttg gggaattact acgcgccctg
    22801 gatgaaaaaa tttacacacc gtactacagc gccctgattc gcctcctgat tgtcttcgga
    22861 tgccgtacca ctgagctaag acgctctgag gtccaggaat gggattttaa agaaatgctc
    22921 tggactgtgc cgaaagaaca cagcaaaacg aaggtagcaa tattcaggcc aataccggaa
    22981 gcgatcttgc cgttcgtaac gaagctggtg gagcagaacc gacacacggg cttattactc
    23041 ggagaactga aagggcaatc atctgtatca gagtacggaa gaacggcaca cagacgcatt
    23101 aatcaagccc cctggacgtt gcacgacatc cggcacacgt ttacaacgat gctgaacgat
    23161 ttaggcgtgg atcctcatgt agtggagcag ctaaccgcgc accagttgcc aggaatgcaa
    23221 cgagtctata atcactcccg ttatctcgat gcaaaacgtg acgctcttaa tctatgggtt
    23281 gagcgtctcg agcttctcca gaacaatgat gaaaaaatcg ttgttatgac cccgcgaatt
    23341 tactcccaaa attcttgaca aattacggct gtttttcttg cgaaaaatgg ccctctaaaa
    23401 taaatcgtcg aatatcggca aatatcgacg atttatatgt gtcaagtatt gaaaaaattt
    23461 gaaatgtctt cttcttagtt cttattctct gtttcttgat tgttccaaca cctgatggtt
    23521 tatctatttg tttttaatga ggtaattcag gtgtcatgcc aaataacgac attttttgtt
    23581 gcgtcgctta aaaactcgtg acacgatttt aaacactaac gagcttctac gaaccttagc
    23641 gaacaaagcc tttagcatgg ttgtgttttt aatttaagag gtgaaaaaga atctaagtaa
    23701 tatccgatct catatatcta cagctaagag aaaagtttat atacgtaatt tttatgtagg
    23761 tttttgtatg cgtgaaataa atgaagatcg cgtaatccgt gaggatgaat gtcgtaagtt
    23821 aactggagtg tgccgcacta cacgttacga actggagaaa aaagggcgct ttccgtcacg
    23881 tcttaatctg ggcggtcgtt ctgtagggtg gctgctgtcc gaggttatgg aatgggttaa
    23941 aagccgggat cgcattaatt caggtaaggc agtgtaaagg ggagcatatg acacataaaa
    24001 caaaggcgac ctggggcggt cgccagtttg gaaacactaa acataagcat ggcaatgata
    24061 gcctctatca ggctggtggg caatgctatc agtctgtttc agtgcgttgc cataactgcg
    24121 aagctctctc ctttgatgag tattctttaa ggtacttctc aagggcaaaa gaacacggcg
    24181 cgaatcgttc tgattcatgc tctttctttc tgcgtcgtct ttttcgtgca ggtgatgttt
    24241 tttcggttga ttcttcgttg gtcattgtgt gcgcctgtaa agcaatgcgc cggagttcct
    24301 cacaccacgg cgctgatagt gattattctg attctttggc cttgcgacgc tggcggcgtt
    24361 tgatctcgcc tttaacggca gtaactataa attgcgcttt gctttcacct tcatctaaat
    24421 ttttttctaa gtctgcaaca acatcatgcg ggaatctggc atttaactgt tgcgacttat
    24481 tatttgttga acctgttgcc attactgaat cctctctaag ttggtgcgat tcagtataca
    24541 caaaaaaaat ttcagaaaaa ggcttgaagt gcgattcact tgctgatagg ttaaaaacag
    24601 atggtgcgat gcaccaaata acaacgcccc gcagtggtgg cacacatgca gggcgtctaa
    24661 ccaccaacga tagcaacagt atcgaggcag ctatgagaaa ttataccaga cacccgcaag
    24721 ggcgggactc gcacaacctg aataaataca tctggcgttt tatcgccctg agcacggcac
    24781 aaccgcgtgt gattaccatc gaggccacca gcgaacagga agcacgccag caatccccgg
    24841 ctggctgcgt gatggtattc gccgcccgta ttcgtcaggg gtcacatcat gcgtaaaaac
    24901 cgcttgcaaa aaattatcac ggggctgtat gcttcccccg tcgcccacat ggcgaccggg
    24961 tttgacagcc tgaatactac agcggacagc cgcttatatt ccgatatgcg gtttttttgt
    25021 gtccgtaagc ctacccatac ccgcattatg gcggggcgta acgggggagc ctttgcgctc
    25081 gctggttcct gtagtgccag tctgtcaacc ctgttacgtc tcgccaccat gtttgacagc
    25141 gtagtagcga gactccttaa tttcactaca ggagcctttc acatggctgt atccgcacgc
    25201 ccttactttg tctggcgctt tatgcagtgc caccacagca gcattgatat ttacaccgtt
    25261 accgctgtca ctgaacgcga agcacgcgcc cagctgccgc acgcacatct gatttttgtc
    25321 gcccgtattc gtcagggggt gagtcatgaa taaaataccc tatgatgttc ttattcattc
    25381 tgaaaacgca ttaattaaag cgcgtgaaat gaatgcgctg ttaattaagt taatagacgt
    25441 gcatgaaggt ggtggagaac ctgcatcatt aatatttgcc gccgttcaga cattactatc
    25501 gcctgtaatt gatgaactgg ataaggcgat ggcaattcac gaaaataata acgcgcccca
    25561 caccggagaa taaaaataat gaaacttaaa tatcctggct taactgccag tggcaaaact
    25621 cgcactaaat tcatgtgcgg ggatatttac cgcgaccagt acggcggcac ggtaatgatt
    25681 aagggcgtgg aggaacggcg cgtaacctac cgccgtgaag gctacgaata tgattgcgtg
    25741 atgcctgttt atcagttcga ccgtgatttt tctctggtgc aggctgtacc gcgtaacgtg
    25801 cccaccagca gggagaaagc acgcgccaat attcaggaaa taagaaagat gcttaacgta
    25861 ttcaggggta aaaaatgaaa ctggcaccga acgtaaaaaa acagccacgc ggaataaaac
    25921 acaaagacac agaggtaatt atctttgcgg gtagtgatgc ctggtcacac gcgaaacaat
    25981 ggcaggaaca tgacgcgcgt atggccggag ataatgagcc tcctgtgtgg cttggggatc
    26041 agcagttatc cgaactggat aagctgcaaa ttgtgccgga aggccgaaaa tcagcacgca
    26101 tattcagggc cggacatctt gagccagtaa tgatcaaggc gattggtcag aagctggcgg
    26161 cggcaggcgt acaggatgca aatttttacc atgagggaat gcacgttaag aaggtggaga
    26221 actggcgcga atatctggcc cgtgagcgcc agaatctttc tgatggtctg gttattgagc
    26281 ttccggtaaa gaaaaaagat accggaagtc atagcgatga cgaattaaag ccacgcgttg
    26341 aaagtcgcgc cgatggtgtt ttctgggtaa cgcccaaagt ggataagcag tcaggcgaaa
    26401 ttatccggcc tgagacgtgg ttatgttctc cgcttgaact actgggaacg gggacgatcg
    26461 gtaaagagca ttaccgcgtg atgcgctgga aaaaaaccgc aaaccatgaa gtcatcacaa
    26521 tggcgatccc gtgtggtggt atcggcgacc gtgacggctg gcggttactt aaagatcacg
    26581 ggctgaacgt gacaaccaac ggcaaataca gggcaatcct ggcggactgg atgcagttaa
    26641 gcggaagcca tgaggaatgg cagttaagca caacaacggg ctggcatttt ggcgcgtaca
    26701 tcatgccgga cggttcgatc attggtgatt ctgaaaaacc gatcctgttt accggaaaaa
    26761 gcgccgcgat taacggttat tctgtggcgg gtacggcgga gggctggcgc gagagtgtgg
    26821 cgcggctggc tggcggtaat gcctcaatga tgctgggtgt ggcaacgtca ctggcagcgc
    26881 ctttaattgg ccttgtgggt gctgacggct tcggggtgca tcttttcgaa cagtcatcgg
    26941 caggtaagac caccacgcag aacatcgcat caagtttatg gggagagccg gacgcgcagc
    27001 ggctgacctg gtacggcacg gcgttaggta ttgccaatga ggccgaggcc cataatgacg
    27061 ggcttttacc actggatgag ataggccagg ccggaaatgc gcgggaagtg tcaacgtctg
    27121 cttatacgct gtttaacggt tcagggaaat tgcagggggc gaaggatggc ggcaacaggg
    27181 agatcaaaca ctggcgaacg gtggcaatca gcaccggaga aatggacgtt gagacattcc
    27241 tcaaaagtga ggggataaaa gttaaggcgg ggcagcttgt ccgactgctt aacgttccga
    27301 tggaaaaagc cacgaaattt cacgaataca gcaacggtaa ggaacatgct gacgcgttaa
    27361 aggatgcctg gacagcaaat cacggggcgg cgggtcgtga gtgggttaaa tggctggcag
    27421 gccaccagca ggaggcaaag gacacggtaa gggaatgccg cgaacggtgg cgcaacctga
    27481 taccggagag ttacggcgag caggtccacc gcgtgggtga acgtttcgcc atactggagg
    27541 ccgcgcttgt gctttccggt catgtaactg gctgggtcgt gcaggaatgc cgcgacgcga
    27601 tacagcataa ctttaatgcc tgggtgaagg agttcggcac gggtaacagg gaatttaaac
    27661 agatggttga acaggctgag gcgtttttgt cgtcgtttgg gtttagccgc taccttccgt
    27721 accccaatac agatgagcgt gatttaccga ttaaagagct tgccggatac agaaaaggga
    27781 gcattcgcaa cgaagatgac gagatgcgct attacacgtt cccgcacgtg tttgagagtg
    27841 agatagcgaa gggatttaat ccggcgcatt ttgcgcgtgc cctggatgct gccggaatgc
    27901 tggagaaggg aagcgatcgc cgatacaaaa aaaaggcgct gggcaagatt gggggtaagc
    27961 agcatgtttt ttacgtgctg atgttccagc ctgatgatga agattaatcc cctgtgtgag
    28021 gaaaaaattg cgggttatgc gggttatcct gtgtataaga gtattaagtg catgaatata
    28081 aaggaaacca ataacccgca cgtaacccgc aaaagggcat ttataacccg caaaagcgcg
    28141 attataaccc gcaggcgaat aactgaagta acaccggaca gtcatgcgta acccgcagaa
    28201 aaaaccccgt ttgcgggtta ttttgagcgt tttgcgggtt acgtggtgag caaataataa
    28261 acagggtgtt ttgcaacata ctgatataaa aggataaaaa atggttagca cgcgaagata
    28321 acccaataac ccgcataacc cgcacaggtt cctatatata taaattgagg taccaaatga
    28381 cccaaaaacg cagagacaga acagagccga aatataaggc gttagatatg gctgaactgg
    28441 ctttaaaggt ggcaatcaga acgatagacc gccacgcggg ggaaggatac gcgaaggaac
    28501 atcccgacct gataagcgca ttcatgacca cagcagcggc aaactttgcc acgctgacag
    28561 aacgggagat tgccgaagcg gaacaggtaa caaccatcaa cgttaaaacc ggagaggggg
    28621 cagtatgac
//
LOCUS       DAZGSP010000065        28455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZGSP010000065 DAZGSP010000000
VERSION     DAZGSP010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.065"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(76..639)
                     /gene="pqiC"
                     /locus_tag="ACHK74_003073"
     CDS             complement(76..639)
                     /gene="pqiC"
                     /locus_tag="ACHK74_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFP0221843.1"
                     /translation="MKKWLVTIAALWLVGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            complement(636..2276)
                     /gene="pqiB"
                     /locus_tag="ACHK74_003074"
     CDS             complement(636..2276)
                     /gene="pqiB"
                     /locus_tag="ACHK74_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFP0221844.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(2281..3534)
                     /gene="pqiA"
                     /locus_tag="ACHK74_003075"
     CDS             complement(2281..3534)
                     /gene="pqiA"
                     /locus_tag="ACHK74_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFP0221845.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(3664..5571)
                     /locus_tag="ACHK74_003076"
     CDS             complement(3664..5571)
                     /locus_tag="ACHK74_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0221846.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAKLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(5582..7690)
                     /gene="rlmKL"
                     /locus_tag="ACHK74_003077"
     CDS             complement(5582..7690)
                     /gene="rlmKL"
                     /locus_tag="ACHK74_003077"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFP0221847.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEDIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            7934..9043
                     /gene="ycbX"
                     /locus_tag="ACHK74_003078"
     CDS             7934..9043
                     /gene="ycbX"
                     /locus_tag="ACHK74_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFP0221848.1"
                     /translation="MVTLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            complement(9040..9582)
                     /gene="zapC"
                     /locus_tag="ACHK74_003079"
     CDS             complement(9040..9582)
                     /gene="zapC"
                     /locus_tag="ACHK74_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFP0221849.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(9756..10766)
                     /gene="pyrD"
                     /locus_tag="ACHK74_003080"
     CDS             complement(9756..10766)
                     /gene="pyrD"
                     /locus_tag="ACHK74_003080"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFP0221850.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(10877..11614)
                     /locus_tag="ACHK74_003081"
     CDS             complement(10877..11614)
                     /locus_tag="ACHK74_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFP0221851.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAIRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTWNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(11580..12095)
                     /locus_tag="ACHK74_003082"
     CDS             complement(11580..12095)
                     /locus_tag="ACHK74_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0221852.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(12103..12645)
                     /locus_tag="ACHK74_003083"
     CDS             complement(12103..12645)
                     /locus_tag="ACHK74_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0221853.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(12657..13727)
                     /locus_tag="ACHK74_003084"
     CDS             complement(12657..13727)
                     /locus_tag="ACHK74_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0221854.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTSVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(13718..16318)
                     /locus_tag="ACHK74_003085"
     CDS             complement(13718..16318)
                     /locus_tag="ACHK74_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFP0221855.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            complement(16343..17044)
                     /locus_tag="ACHK74_003086"
     CDS             complement(16343..17044)
                     /locus_tag="ACHK74_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0221856.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            complement(17127..17669)
                     /locus_tag="ACHK74_003087"
     CDS             complement(17127..17669)
                     /locus_tag="ACHK74_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0221857.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            18025..18600
                     /gene="ssuE"
                     /locus_tag="ACHK74_003088"
     CDS             18025..18600
                     /gene="ssuE"
                     /locus_tag="ACHK74_003088"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP0221858.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            18593..19552
                     /gene="ssuA"
                     /locus_tag="ACHK74_003089"
     CDS             18593..19552
                     /gene="ssuA"
                     /locus_tag="ACHK74_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFP0221859.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEIAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            19549..20694
                     /gene="ssuD"
                     /locus_tag="ACHK74_003090"
     CDS             19549..20694
                     /gene="ssuD"
                     /locus_tag="ACHK74_003090"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFP0221860.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            20705..21496
                     /gene="ssuC"
                     /locus_tag="ACHK74_003091"
     CDS             20705..21496
                     /gene="ssuC"
                     /locus_tag="ACHK74_003091"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFP0221861.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            21493..22260
                     /gene="ssuB"
                     /locus_tag="ACHK74_003092"
     CDS             21493..22260
                     /gene="ssuB"
                     /locus_tag="ACHK74_003092"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP0221862.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(22467..25079)
                     /gene="pepN"
                     /locus_tag="ACHK74_003093"
     CDS             complement(22467..25079)
                     /gene="pepN"
                     /locus_tag="ACHK74_003093"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFP0221863.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            25345..26547
                     /gene="pncB"
                     /locus_tag="ACHK74_003094"
     CDS             25345..26547
                     /gene="pncB"
                     /locus_tag="ACHK74_003094"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFP0221864.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            26716..28116
                     /gene="asnS"
                     /locus_tag="ACHK74_003095"
     CDS             26716..28116
                     /gene="asnS"
                     /locus_tag="ACHK74_003095"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFP0221865.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         6895 a         7450 c         6906 g         7204 t
ORIGIN      
        1 ttttccgcac gttagaaaca aaaaaaccgc ctcaggcaac ataccggaga cggttatatt
       61 tacaaatctt tataattaag gtagacgctt tatctcttgt gcaatagaag cggcttcctg
      121 actccagaca ccggccagca ctttaaccat ctcatcgtaa ccatcctgag tttgcactcc
      181 ttccagacgg aacggacgtt tgatcagttg tccctggtgg ttcaacagcc actcaccact
      241 gacaatgacc ttgccatcat agcgaccgtt aaactccgtt acggtaacat tgagcgtgtc
      301 ctgggcgctt cccagaggct gggaggcaac cacccagccg ggcagttgcg tactcaggtt
      361 ggcaaccagg gtgttgcgca actgttgatc caacgggctg gcccacaagt tgttgttggc
      421 aatcacatac ttcacatcac tggtttgata aaccacacca ttccccgcca gatagtcagg
      481 aacagtgacc tgctctaccc ataacagacg attgccctgg ctggcggtac tttgtgtacc
      541 gctctgcacc acaggtaact ggtaatagtt tttattaatt tcgccggagc tgcatccgac
      601 cagccacagt gctgcaatcg tcactagcca ctttttcatt gtttcgccct cttcggctct
      661 ggatcttttt tgtccttcgc ttcaaatacc agcgcgttac tcttctcatt gagcgttttc
      721 agcaccggtt gcagttctcg caacacctga tcaaggcgct gcatatccgc caccatcttg
      781 ttgtaggctg cggagccagg ctggaagccc tgcatgctgc gattcaattc acgcaacgtt
      841 gactgcatat ccgtcggcaa ctgctgcatc gactggctag cgaggatctt gttcatgcta
      901 tccagcgtcg tttgcaggtt tttcattgtg cgctgacttt cagaaagcgt actggttgcc
      961 tgttcaatca tcggattcaa tggcagtttg ttgatcttat ccaacgcttc catcagtcgt
     1021 tgctggattt gcgccaggcc gccgctaacg gtcgggataa tctgataacc attaaattca
     1081 cgaataccgg ttattgcagg cgtatttgga tagaaatcga gatcaacata cagtgcacca
     1141 gtgaccaggt ttccggtttt cagcgatccg cgtaaaccac gtttcaacaa ttcgccaagg
     1201 tgctcaacaa catccgcatt ttcgccaagc tgcattttca gccgctctgg ctcgatacga
     1261 atcagtaccg gaatacggta atcatcgtta aatgtctgac gcatattcgg cgcaaagaat
     1321 ggcactttgc ttacggtacc caggcgaata ccccggaact ctaccggagc tcccggttgc
     1381 agaccgcgta ccgaatcttt aaagaacatc agataatcaa tgtgatcggt gtacaacgaa
     1441 tcctgaatgc tcttctgatc atcatacaaa acgaaagctg ttttcggtgc cactggctgc
     1501 cctaaatcca gaccttccgg cacatcaaag ctgacaccgc cactcagcag cgttgtcaat
     1561 gagcccatct ccacacgcat ccctgctgac gtcagatcaa ccgcaatgcc actatctttc
     1621 cagaagcgaa cattgttggt caccagtcgg tcataaggtg cattgatgaa cagttgatag
     1681 ctgatattgc gtttttgtgt atcgaaggtg ctggtttcaa ccgaacctac ccgatagcca
     1741 cggaacagca ccggatctcc tggcgagagc tgcccggctt ttttgctatc gagaatcaca
     1801 cggatgcctt tcgcatcagg cggggccaac ggtggcgagt ccagcaaatc gtatttatcc
     1861 attttgctgc ctttcgcgcc tggctgcagt tcgatataaa ctccagacag cagcgttccc
     1921 aggccgctaa tcccttcgcg accaatctgc ggtttcacca cccaaaagac ggtgtcttta
     1981 tgcagcaatt tttccatacc ggaattcagc cgcgctttga tttcaacgtg cgtcaaatca
     2041 tcagccagtg tggcgctttc aaccacgccg acgtcaacgc tacggctttt aatggtggtt
     2101 ttgccacctt caattccttc cgcattcgcg gtgatcaggg tcacttccgg tccctgatgg
     2161 ctgtaatgat aaaaaagaac ccaggcccca atgagcgccg tgacgatagg aaatatccac
     2221 acgggagacc agttcttcac tttctggatt ttggcttccc cattattaga ttccatactg
     2281 tcaggactcc tcatgctctg attcaggttg acgatcccac gacaaacgcg ggtcaaacgt
     2341 catagcagaa aacattgtca ttatgacgac taaagcaaac attaatgcac ccattgccgg
     2401 ataaatactc attaaacctc ccatacgcac cagcgccgag agcaccgcga taacgaaaac
     2461 gtcaatcatc gaccagcggc ctacaaactc aacaacttca taaatcaaat gcattctttc
     2521 actgtcgcgc ttgccatgcc ctttggcatc ccaacacagc cacgcgatgg cgatcatctt
     2581 taacgttggc accataatac tggccagaaa gatcaccgca gcgacgggat aagagccttc
     2641 gctccataac agaatgaccc cagcgagaat cgtcgacggc atcttcgagc ctaataaatc
     2701 cgtcaccatg atgggcaaaa tattagccgg aagatacagc atgatggacg ttacaagcag
     2761 cgcgagtgtc cactgcaggc tgtttctacg ccgaacgtac cctttggtac tacaacgcgg
     2821 gcacacgggt tcatcagcag gaaggattgc cgtacaacag gagcacgaac gcagcccctg
     2881 acgtatcccc gtgacgcctg gttttagcgg ctggcgcagt tctggcatcg gggcgatgtc
     2941 gtcccataac cagcgacgat caacgcactg aaaagcgcgc agttgcagga cacaaaataa
     3001 acaccagggg agaaagctgc tgcctacccc aatgctgccg taagccatca gtttaacgaa
     3061 actgaccagc acaccagcga ggaaaatctc cgccattccc caggttttga gttgaaaaag
     3121 cacccgtgcc agttgctctt ttaaacggac cggtaattcc gcgcgattca ccagtaacag
     3181 aatggttatc agacaaaacg cgggaaccag ttgcacaaac aacaggaaaa aggtgccgag
     3241 gctggcgtag tcctcagaaa aaagcacgcc gggaatttcc agtaatgtaa tttcactggt
     3301 aactcctgca acgttcatat tcacaaaagg aaacaagttg gacagcaaca gcatgaacag
     3361 tgcagccaac gcataggcgg tgggacgctg ccgaggggca tcccacgcca cggttaacgt
     3421 tgtgccacac cggggacatg ccgctttctg accatgctca aggcgcggta acgccaccag
     3481 catgtcacac tgcgagcaca ggatgtgctt cgcggcatga tgatgttcgc acattggtac
     3541 tccttaatga tgcgctattt tacagatgac taacaaagtg ttggttttca tgcctgataa
     3601 acccagttca tcaggcaatt ttaccgttgt cagctgtctc tgtttaaatc gactattttg
     3661 cgatcagcca ccatttttta acgcttcaag atactcccag cgttcaaagg cttgctccag
     3721 ctcctgctct gcagcagcca tatcggcgag cactttttgt gtctgctcat gtggctgact
     3781 gaagaaggaa gcatccgcca cctgcgtctg tagggcttcc agcttcgcct ccagatcttc
     3841 gagcaattgc ggtagctgct ccagttcgcg ctgcaattta tagcttagtt tgctactgct
     3901 gcgttttaca gtttctgctt ttgccgcggc ggcttcttcg gtttttttca ccgcaggctg
     3961 tttgagcgcc acatactgct cttgctgacc acgggcatca tgataaccgc cgacataacg
     4021 accaatttta ccgccgcctt cgaagatcca acattctgta acggtgttat cgacaaactg
     4081 acgatcgtgg ctaaccagca ataccgtgcc ctgatagctg tcgatcagtt cttccagcag
     4141 ttccagcgtt tcgacatcaa gatcgttggt cggttcgtca agaatcaata agttgcttgg
     4201 tttgaggaac aaacgcgcca gcagcaagcg gttccgctca ccgccagaaa gcgcacgtac
     4261 cggcgtcatc gcccgtttcg gatggaacag aaagtcctgc aaatagccca atacgtggcg
     4321 tggcttgccg ttaaccatca cctcttgctt accttcggca aggttatcca tcaccgtttt
     4381 atcgggatcc agttccgcgc ggtgctgatc gaaataagcc acttccagtt tggtgccaac
     4441 gtgaatacgc ccgctgtccg cttgaagctg accgagcatc agtttcagca gcgtggtttt
     4501 gccgcaccca ttcggaccaa tcagggcaat tttgtcgcca cgtagaacct gggccgaaaa
     4561 atctttcacc agttgcttac cgtcaacctg gtagcaaacg tcttccattt cgaaaacgat
     4621 cttaccggag cggctggcct cttccacctg catctttgcg gtacccatca cttcgcgacg
     4681 ttcaccacgc tcgcgacgca tcgctttcag ggcgcgtacg cggccttcat tacgggtacg
     4741 gcgtgctttg atcccctggc ggatccacac ctcttcctgc gccagtttgc gatcgaactc
     4801 ggcattttgt aattcttcca cgcgcagggc ttcttctttt tccagcaggt actggtcgta
     4861 attccctgga taggtcacca gcttgccgcg atcgagatca acaatgcgcg tcgccatatt
     4921 gcggataaac gaacggtcgt gggagatgaa aataatcgtc ccgttgaaag ttttcaaaaa
     4981 cccttccagc cagtcgatgg tttcaatatc caggtggttt gtcggttcat caagcaacag
     5041 cacgcgcgga ttactcacca gcgcgcgtcc taatgccgct ttacgcaacc agccgccgga
     5101 aagcgacgac agcgcaacgt taggatctaa ccccagtttc gccagcactt cgttgatgcg
     5161 gttttccagc tgccacaggt tgtggtgatc cagctgttcc tgaaccttcg ccagttcgtt
     5221 gagatttttc tcgctcgggt cgttcatcac caggcgcgaa atatcgtgat agcgtttcag
     5281 atattccgct tgttcttcaa tgccttcggc aacgaaatca taaacgctac cctcaacgtt
     5341 acgcggcgga tcctgttgca gacgagctac aatcaaatct tgctcgtaaa taatgcgacc
     5401 gtcatccagc ccttgttcac ggttgaggat tttcattaac gtcgatttgc ccgcgccgtt
     5461 gcggcccacc agacaaacac gttcgttatc ttcgatatgc agttctgcgt tatcgagaag
     5521 cggcgcgtcg ctgaacgaca gccatgcgcc atgcatactg attaatgaca ttactattcc
     5581 tttaggctgc ggtaatcagc cagcagttgt ggatctggcg gttacgggcg aaatcctggg
     5641 agagcgtttt ttgcgtaatt tcttgtgctt tcagtcccag ttttgccagg ccgtcgagat
     5701 ccatacggaa gccacgttta ttgttcgaga acataatcgt cccacctgca cgcagcagac
     5761 gtttcaaatc tttcatcagc gccagatgat cgcgctgaac atcaaacgca tcttccattc
     5821 gttttgagtt agagaaggtt ggcggatcga taaagatcag atcgaactgt tcatttgcct
     5881 cacgcagcca cgccaggcaa tcggcctgaa tcaggcgatg cgcacgcccg gtcaggccat
     5941 tcaaacgcag gttgcgttct gcccactcca gataagtacg cgacatatcc acggtggtgg
     6001 tgctgcgtgc accgcctaat cccgcatgca cggtggcgct gccggtataa gagaacaggt
     6061 tgaggaaatc tttgcctttg ctcatctgac cgagcatacg acgggcgata cggtgatcga
     6121 gaaacagacc ggtatcgagg taatccgtca ggttcaccaa caagtgagcg ttatattcgg
     6181 taacttcaag aaactcgccc ttctcgccca gtttctggta ctgatttttg cccttctggc
     6241 gttcacgggt tttcagcacc agtttgtttg gcgcaatccc cagtaccgaa atggttgcag
     6301 cgataatatc gaacagacgc tgacgcgctt tgtgagcatc aatagttttt ggcggcgcat
     6361 actcctgcac caccacccag tcggcataac ggtcaacggc aacgttatat tccggtagat
     6421 cggcgtcata cagacggtaa cattcaattc cttcctggcg agcccacttc tcgaattttt
     6481 tgaggttctt acgcaggcgg ttggcgtagt cttccgctac catcgccggt ttgctgtctg
     6541 gagtgctttc ggcaacgtgg taatttttct gtacgcagtc cagcgggccg tttttcgcct
     6601 tgtactgttt gtctgcacgc agctgcaagc agcttagcag atccggcgag gcactaaaca
     6661 aagagagatt ccagccacca aactggtttt tcatgatccg acccagcagg ctatgcagcg
     6721 caatcagcgc cggttcgctg tccagacgtt ccccgtatgg cgggttgctc aacactgtgc
     6781 cgtacggccc tttcggcagc ggattggtca gttgcgcgac atctttcacc tcaaaggtga
     6841 tcagttcacc aatccccgca agacgggcgt tagtgcgcgc acgttgaatc acccgtgcgt
     6901 cgctgtccga accgtaaaaa tgagagctat actcagccag gcctttacgg gcacgagttt
     6961 gcgcttccgc tttcacttcc tgccagatat cttcatcatg ctgcacccag ccgctaaagc
     7021 cccaacgccc acggtgcaag cctggtgcgc gatcggtcgc tagcatcgcc gcttcaatca
     7081 gcaacgtacc ggaaccacac atcggatcga gcagcggtgt tcctggctgc cagccggatc
     7141 gcatcacaat agcggctgcc agggtttctt tgatcggcgc aataccagca cgatcgcgat
     7201 agccacgcag atgtaaacca tcaccactca gatcaagagc gatactggcg gtttctttat
     7261 gcagccagac gttaacgcgg atatccggcg catcgcgatc aacatttgga cgcggcagat
     7321 ttttccgcgt gaaagcatcg acgatcgcgt ctttcacttt catcgcgccg tactgactgt
     7381 tgcggatggt gtcattcaaa ccactgaagt ggacagcgaa ggtcgcgcca ggattaaaca
     7441 tctctgtcca gttgatcgcc tgaacaccga gatagaggtc taaatcgctg taaaccttac
     7501 actcgcccag cggcaacata atacgcgagg ccaggcggct ccacatcagg ctctggtaaa
     7561 caagccgtgt gtcgcccttg aaatggaccc caccctgaac cacctggcat tcaacggccc
     7621 ccaggttttc cagttcagtt tttaacagct cttccagccc acgggccgta ctggcaaaca
     7681 gagaattcat atcgtcactt ttactctaag agcctaccgc agtaggcgta attgttgcag
     7741 tcagtctgga catggacagc gcggagaaac cggagcgtac acaagtacgt gaggatttcg
     7801 agcacaaccc agggccaaac tgacaaataa aatagcctaa tgggacaggc gctaagaaaa
     7861 ttgttgcgca ttatagctaa taagcgcgtc atgtcataaa gttgagggct tattttcatt
     7921 tgaggaccgc accgtggtga cattaacccg gctttttatt catcctgtta aatcgatgcg
     7981 cggcattggt cttacacatg ctctggctga tgtcagtggt ctggccttcg atcgcatctt
     8041 tatgatcacg gaacctgacg gtacgtttat taccgctcgc cagtttcccc agatggtacg
     8101 gtttactcct tcacctgtgc atgatggctt gcatctcacc gcaccagatg gcagtagcgc
     8161 atatgttcgt tttgctgatt tcgccacaca agacgcacca accgaagttt ggggcacaca
     8221 ttttaccgcg cgaattgcgc cagacgcgat caacaaatgg ctaagtggat ttttctcccg
     8281 cgaagtgcaa ttacgctggg tggggccaca aatgactcgg cgcgtgaaac gccacaacac
     8341 tgtacctctg tcatttgctg atggctatcc ttaccttctt gctaacgaag cctcgttacg
     8401 tgatctccaa caacgttgtc cggccagtgt aaaaatggag caattccgcc ccaatctggt
     8461 ggtttccggc gcgtcagcct gggaagaaga tcgctggaaa gtgattcgca ttggtgatgt
     8521 ggtgtttgat gtggttaaac cttgtagccg ctgtattttc accaccgtca gcccagaaaa
     8581 agggcaaaaa catccggcag gcgaaccatt aaaaacattg caatctttcc gcactgccca
     8641 ggataacggc gatgtcgatt ttggtcagaa tttaattgcc cgtaatagcg gcgtgattcg
     8701 cgttggcgat gaggtggaaa ttctggcaac ggctccggca aaaatttacg gcgcagctgc
     8761 cgctgatgat accgccaaca tcacgcaaca accggacgca aatgtagata ttgactggca
     8821 gggacaggca tttcgtggaa ataaccaaca ggtgttgctg gagcaattag aaaatcaggg
     8881 aattcgtatc ccttattctt gccgcgcggg catttgtgga agttgccgtg ttcagctttt
     8941 agaaggcgaa gtcacgccgc tgaaaaaatc agcaatgggc gatgatggca ccattctttg
     9001 ctgtagctgt gtaccgaaga ctgcacttaa gttggcgcgt tagactgcct gttcgaggct
     9061 gaagctgtca acattaacct gcggtttcag cctgtcgttc atgattttaa tggcgtcgcc
     9121 caactgcatc gcgcgacccg caataacaac gcagggttgt gccagcaggc atagtgcggc
     9181 attttcgcca gattcaacga ccagcaaatt gacctgctcg tgagtatcgc tcaaccaaac
     9241 gcaggccgca tcgcccggca tgggcaccca catttcacca tgggaaacaa aatgccagct
     9301 tttcggcatt tgcggtttga gatagcgaat tgctaccagt gcattcagca ccagttcggc
     9361 tttttgttct ttagataaat taaaatcgcg gcacttttct tcaaaagaga aatagagcgc
     9421 ggcgtcgtca acgcaaaagc ctgcgggaga aaaggcatct ggagtcaaca tcttgcgcgc
     9481 aaaacgtgag cgaaatagca taccattggc taaatcgagc atcattcgat cgtgctcttc
     9541 atcgtaatac caacgccagt tatcgtctgg tttaattcgc atctgcctct cccctaccct
     9601 ataaaaccgc agcaacacaa tccattgtcg cttcgcttaa taacctcaac aataaaagta
     9661 caaaatgtct aaataagcaa cagcaatgaa tataaaacaa ccagggctga aaataaagcc
     9721 ctgattatct tataaagagg aattcgaaga aataattaga tatgggtaac gatttcttta
     9781 atcagcggcg gacctttaaa aataaaacca gaataaattt gcaccagtga ggcacccgca
     9841 gcaatctttt cacgcgcagc gataaccgag tcgatgccgc caacaccgat gatcggtaag
     9901 cgaccgttta attccagtga caagcggcga ataatttcgg tgctttttaa ctgaagcgga
     9961 cgaccactta agccaccggt ttgatcgcaa tttttcattc cctgaacaag agaacgatcg
    10021 agtgtggtat tggttgcaat aacgccatca atattatggc gaactaaact atcggcaacc
    10081 tggatcaatt cttcttcaga aagatccggc gcgatcttca ctgcgatcgg cacatattta
    10141 tggtgcatcg cttgcaaatc attttgctta tttttaatcg cggttaagag atcatccagc
    10201 gcttcaccat attgcagtgt gcgtaatcct ggggtattcg gcgatgaaat attgatggcg
    10261 atatatcccg cataggcata gattttttcc atacaaatca gatagtcatc tttgccctgc
    10321 tccactggcg tatctttatt tttgccgatg ttaataccta ggacgccgtc ataatgggcc
    10381 ttttttacgt tctctacgag gttatcaacg ccaagattat taaagcccat acggttgatc
    10441 aaaccttcgg catctaccag acgaaagaga cgcggcttgt cattacctgg ctgtggacgt
    10501 ggcgtgacgg taccgatctc gatcgatcca aatcccatcg cgcctaacgc gtcaatgcac
    10561 tccccgtctt tatcaagacc ggctgccaga ccaagcggat ttttaaacgt caggcccatg
    10621 cagttaacag gtttcgcagg cactttctgc cgcaccagtg cttcaaacgg cgttcctgta
    10681 atacggcgta attgctgaaa agtaaactca tgagcgcgct ctggatcgag ctggaaaagg
    10741 gctttacgaa cgaaggggta gtacatgaac tctcctggat tcccggtgtg caaaccgggg
    10801 gcgtattatg tgcgagttcg gaccaaaagg gaattgacct gcggcaaaaa aaacgcaaac
    10861 gttttccgaa cctgtttcag ggcagatttt gctccttctt tgcactcaac ccgccgtaat
    10921 catttatcgc ccgccaggcg acatgacacg gtgcgattgc ctgacaccag cttgtctgac
    10981 gctggctttt aggcggcaca actcccgcat tacttaacgc tttaccattt acgctcacct
    11041 gaatcaggtt aatgtagtaa ggcgttgggt tagtgagttg tacaccctgc gaattccatg
    11101 tccagcgtaa ttgttgataa gcttccagcg ggtcgcccgg caaaccttcg ggtcgccaga
    11161 acagtttaaa taccgagcgt atcgccactt ttacgctctg attttcaact ttgccggatg
    11221 gcacgctggc aatgcttaac tcaaatagcg tttcgcgatc cacaggcaag atgtcgcttt
    11281 ccgttctcag caagcgcaac gtgcctgtag taccgggctt caggagaata agtggcggag
    11341 cggccagtaa tggtgctggc actgtcgacg cttccccccc ggcccaacgc gttgggcggt
    11401 tgattttgct gttaataagc caggattcct gactggtatt agtcagtaaa atagatatcg
    11461 attctctgtc tgccggaaag ataaatcgcg ttccgccgac caccactcct gcctgcaatt
    11521 cacccgccac cagcagcgat aaaacggcga aaatcaagag cgccaatctg ttccacgtat
    11581 tagtcataat gaagtacaaa tgtcgcgctg gcattcactt tacccgactg cacatcgcta
    11641 ttgacaggtc gcaattgcgc ataaaaagtt aatggcacct taccgctatg taggctgtag
    11701 tctttactcg cctggttaag cggaatcaat atcttcgcat catccagaat cgcaattgcc
    11761 agcccgctgg ctgcatccgt accactttca atggacagca tttcgctctg cactccgtcg
    11821 ggggttcctg aaaaggtgac gtcaaccgtg ctgacatcag ttccgcaatc ctgcaaatta
    11881 atgatgaaag atttctccgg gccataattc ccatgagaaa aacgtgtcag cgaaacatcg
    11941 cctaactgca cattgatttg cttgctatcc tgtgccagca cgcatgtatt acggcgaaac
    12001 gatccgctaa tgttgaccgt gccatcatgg gcaaatagct cacagccccc cgttaatccc
    12061 agtaagaaca gaatccagat tatccgtttc aacatcactc cttcactggt aaaccagatc
    12121 aaaatagaga accgccgtcg cattaccgcc cgttgctccc gcctttgtgg acttataacg
    12181 aagttgatat ttgagtgtgt tatccccggc ttttaagggc gtaagaggct ggacctgatt
    12241 gagcgggatt tcttgctgac tttgcgcatc aagaatttgc acggcaatcc ccgttgccat
    12301 accaccgctt ccgcctgtgt cagttagttt gagcagtgtc ggcgcaaggg tgtcagcctc
    12361 gccgctaaaa aggacgtccg cgccagtaat accagcggca caaccggtga tatcgatatt
    12421 caaatcagca gcagtgctta cggtattcgc ggcctgaaaa tcactgatat taaaatcacc
    12481 aatatgaata ttttgtaatg cacttttggt ggcgacatca cagccctgat cgaccacatt
    12541 cccataaata tgcatactaa cttgttcagg cccagccatc ccgacatgga tgttaagaat
    12601 gctaaagaac aagataacgc actgaaatat cgtttttttc ttcatacgtt cacgcgttaa
    12661 tcaaaattta ttctcaccgt tgccgtggct tcaaatcgcc cggtttctgg tggtacacct
    12721 gtcgtgctga ctggatagac attcattcgc gcgatactgt ctgcatcatc aggaatagag
    12781 agcggtaatg ttccaccagc aggggaaata atgttgttct ggctatcggt caccaccacg
    12841 cccacgccag gacgtgacgt cttaatcgcc tggggatagc tggggtctgt ggttgccgtc
    12901 agactcaggt tgacgttaac taaccccgcg tttcccgtgt tacacctgac tggaattgcg
    12961 agttctgccg ttctggcccc aaggggttta tgccctacaa cacgaaagtt agccgcaaat
    13021 acgtcgccca gatcgacctc gatagtctct ccggcattga cggaacatga gccaagtgaa
    13081 ttgatcgtac cagacaggta atacactaaa accgatgtac ctgtagtcgt gcaggaatca
    13141 cctgcgggta tgttgtagca ttcaaataat cgcgccagcg ccataggagg aatgacgacc
    13201 gaaccaacga acggctgcaa tattttcagg gaaaagctac cgctattacc gctagccccc
    13261 tggccaccta aacgttgacc gccgggatcg caaatatgcc atcgtgtatc tgcccctgtg
    13321 cccacgtttt cgaaaggaac cgcagtagga gaactgcctt taacctgcaa taaaactttg
    13381 gcggcaagat agtcattaat tttgtaccag ttgtcgccta aatagactaa cggtgaatcc
    13441 gcggcgaatg cccatcggcc acttctgaca ttgtctttat cgcaatcaca ggaaacataa
    13501 taatcattcg attcgttcca ctgataaaat ttgtctttaa ctactccagc cgtattttct
    13561 tcttcctcca gggttttaac gccaaaattg acgccaatat acggattccc ctgagtggta
    13621 tgacactgcc cgctataagc agcagtttga gcgcaccata gcattaagac ggcggcaaaa
    13681 aatagtcgta gtaataacac gcatttttct ccaaagatta tctgcatatg gcatccattc
    13741 gcgtaatcgc agagttaatt tgttgttcgg taagctggta actgatatga caaatttgat
    13801 cgggagcttc gccccagcgt acagttatcg ctccctgagc gggcaaaccg gacatataga
    13861 gcgagccatc atcatcaata atgccgctat tagtctgtat gccgtccagc gtcgctatcg
    13921 cgccaaaacg cagcggtata ccctgctttg atgttttcat taataccctt gctcccgcat
    13981 gggcattgaa ctccgccaga accaacgccc ctttggtggg aaccacgttg actactgcat
    14041 tttcaagatc cacgttacgt tttaacgatg ccgcatcaag tgcgattcga ttttcccgat
    14101 aagaagtggc ataggggaca acggtatatc cgcgccagtc agttttcagc ccggtgctgt
    14161 tcgccacacg agtattctct gcgcctggtg ctgcaatcag cacgttagtt tcccccagcg
    14221 attgccccag ggtaatgccc tgtgaatggg caactaaact gcctgaaaga gcgtagttca
    14281 gttgttgttg actgccgtta tcgctgtagt tgtagcccac tcgggcatct gcaaacgccc
    14341 ctttgtagtc catgctggcg ctaccattag ccgttttccc ctcgctgtta tatccctgtt
    14401 gcacgctgta gctcaggtta ccgtcatcga gtagtgtctc cgttaaaccc aggttttgtg
    14461 taatgtgccc ttcgttatcg atactggtgt tagaggtcat ccaggcgtaa ttttttttcc
    14521 tggtatagct atcattactt ctggggagca acagattgag cggcagggaa aaatttagtg
    14581 caaacacctg atcagcatca ggttgaccac gggacttact gtagttccag gaaaccccca
    14641 gcgagagatc tttaatttgc gtgttgtagc caaactgtaa tagccgatcc tgttgatcgg
    14701 tatgccagta agtttgctgg ctaccactta aataaaacga accgtactcg cctaattgct
    14761 gggagatatt gacctgcagt ttgccacgtt tggtgtaaaa caaattgtaa taacgcgacc
    14821 acatcggcgt atcttcatca tcaccgtcat taaattcgta gccatccata tgtttataca
    14881 tggtgtcgga aagggtgtag aaacccgacg tcgaatagcg atagcccatc aattgaaaat
    14941 tagtgcccaa ttggttgagc gttttggcat acagaaaacg atatgactga cctttatgtt
    15001 cgctttggtc tgccagcgta ctcttcgcct gggtcgcatc gaacgaaatt gctccgaaat
    15061 cgccaaggtt aaatcccaga ccaaacatgg cggcacggta atattcagca tattgtccac
    15121 caccatacca tgtcgttccc cacggtccgc cccactgcaa cgtggcctgg gcaaatttgc
    15181 tctcctgctg ttcattacta ttggttctgt atttcgccag tgtcaccgcg tatttgattc
    15241 gcccctgacg ctggagtaat ggcacgctgg aaaacggtac gctgtagcta ttgacgctgc
    15301 cgtccgcttc tttgatttca actaacaaat caccgctcga cgacgtggaa tagagatcac
    15361 taatttcaaa agcaccaggc gatacataag tttggtaaat ggtgtaacca ttttgcttaa
    15421 tcgttatctg ggcattgctt ttggcgatac cacgcaccac aggcgcaaac cctttcaggc
    15481 tgtctggcag catgttgtca tctgacgcca gctgcacacc acgaaagctg acactatcga
    15541 aaaaatcgcc tgcagtataa tcatcaccta cggtcagttc gccttttagt ggaataaccg
    15601 cccgctgcaa atagctgctg agattcttcc attctgcggt ttggccacta ctgcgactcc
    15661 atgttgaatt gttgcgcaat ctccatgggc ctaaattaac gccactgttt aaattcagaa
    15721 aatagctgtc gccagaatca ctgtctgcgc tgctatgaat actgttagcc ccgctaaacg
    15781 aatatcccag taatagcgcg ttgatccctt catcccaacg ttcagggggg acataaccac
    15841 gggcattact tttgatggcg atttgcggta ctgaaatttc cagacggagt ttattcacat
    15901 taaacgttac gctggcatcc ggaataatct ccgccaatgg cacacacgct tgtttgtcca
    15961 gttgtttaaa agccggaaac gcatcagtat taacacccat tgcatccagg ctttcagtcg
    16021 tcaaacaggc ggcaatcccc ttctctggcg ttatctcaac aaaattgacg ttgcgcgtat
    16081 ctacaattgt ctgattcaga acaatatcga cacgataaac ccctggcgtt attttttgtc
    16141 ctttttcgaa acgagacaaa tcggccacca ttgagggatc atctttgaaa aaagcaggat
    16201 cgaaccaggt ttcagcttgt gcattgaacg ctgccgacac aaacaccacc agcccaccaa
    16261 taaacgatgg cactccacca gagcttaaca ggctgtgttg tcgatgagtt ctatacatct
    16321 cgctctctta actgaagacg gcttacgatt tacagttgag ttgcggcgtt tccgccccgt
    16381 agtcattgat ggttcgatac gttacgctgc tatttttggc cccctttggc agagaaaatt
    16441 ggtatttatc aaagggggaa atcatttgat ttttaactac ttttccacct attttcaggt
    16501 ctgtcagggt aatgaaataa ggtgttgggt tactgaccgt aaccatgtca gctgacgaag
    16561 tgcaacgcag tttcgccgga gcctcgccta agggatacgc taaacctttt ggtcgccaga
    16621 aaagtttgat gacgctttgt gtggcaatct tcagggtatt tccctcttcc cgcttcgttg
    16681 ttgaagggac tgcccgtacg ctagtgaaga aaagggtttc tctgtctttc gccaacggcg
    16741 caccaatgta cataatgcgt aacagattct cattagccgg attcagcaca aatagcggtg
    16801 gtgtaataat aaaatctgct gattttttat tatctggggt catcacccat gactgaataa
    16861 gataaggaac atccggactg gtgttacgta cacccagtga aatttgctct tcaccttctg
    16921 aataaacaag acgtgttgct gcaagtccaa tcccgccttt acctgccgcc catgcggatg
    16981 agcagctgag taatattgcc gttaaactta cggtcacaat tccctttgtt atgcaagttt
    17041 tcataaatag tcactcttaa aaaatatgaa tatcccctcc agaacactag aggggtacta
    17101 attaataaaa aaattaatga gttctattaa taatagttaa tatggaaatt tgttaccgca
    17161 ttcacattac cagaagtcgc tttccccgta gcgatataat caactttaaa ggtcactgtt
    17221 tgctcagcag aatccgctaa ctgaatattc gacgtttcgg taccgggagt taccgatgta
    17281 gaaccatctg gcagatacat ttgtagagca acattcgtcg ctgcatcggt ggtagcctgg
    17341 ttggcaaatg ctgtaggttg cacgccgtca gccgtaccac taaaggtaaa cgtcgcattt
    17401 ttagttacag taacatcaca gccttctaag gcaattggta catcagtttt aacgccagag
    17461 actgctccat tagtttgcaa cttggtggcg ctgacatttg gcaatgtgac tactgaatct
    17521 ttcgtcgcag ctaccagggt acaagctgga gcaataactt taccattaaa tgttactttg
    17581 ccatccgtga tagcattatc atcagctgcc ataacgctgg acgttgcaca taccacagtt
    17641 ataaacgccg tcaatacact ttttttcatc gtcatcatcc tgatctcttc gtaaatatga
    17701 taatgccttt aaatgacatt agacttaata ataacaataa ttgaccgcca tttaaatcaa
    17761 gcatactaaa tccaataagt gacaaatctt taatttatag atttcatgaa tatttaaata
    17821 ttaactgaac gagtaattca ttgattcaac atctaatgac tatccaacaa ataaatatgc
    17881 gtgatatata aagccctttc tttagtttat tttcagaaaa agatacacaa aatttatatt
    17941 tggcattttc ttgtctctcc gacagactga ccgaattgtt atcaatgtta acaaaaaaag
    18001 aacaattggt tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt
    18061 ccttctcgct ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctcgatgta
    18121 gaggtttatc actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc
    18181 gatagtccgg cactcaagac cttcaccgaa cagctgcaac aggctgatgg actgatagtc
    18241 gctacacctg tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg
    18301 ccagagcgcg ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc
    18361 catctgctgg cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag
    18421 atcctgcacg gcgtgtttgc cgatgactca caagtaattg attaccatca cagaccccag
    18481 ttcacgccaa atctgcaaac ccgtcttgat accgcgctag aaactttctg gcaggcattg
    18541 caccgccgcg atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa
    18601 catcattaaa ctggcgctgg tgggattgct tagcgtctct acgattgcgg ttgctgcaga
    18661 atcctcgcct gaagcgttac gtataggcta tcagaaaggc agtattggta tggtactggc
    18721 aaaaagccac cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt
    18781 ccccgcgggt ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac
    18841 cggggatatt ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt
    18901 cgagccaccg aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac
    18961 cgtagccgat cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct
    19021 tttactgcgt gcactgcgtc aggccggact taagtttacc gatatacaac ccacttacct
    19081 gacacctgcc gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg
    19141 ggatccctac tactccgctg cgttattaca gggcggcgtg cgggtgttga aagacggcac
    19201 cgatctcaat caaaccggat cgttttatct ggcagctcgc ccctatgcag aaaaaaacgg
    19261 cgcttttatt cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca
    19321 gcgcgagcaa agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc
    19381 ctcttactta gatcatcgcc ctcctaccac catcaaaccg gttaacgccg agattgccgc
    19441 cttacagcag caaacggcag atctgtttta tgaaaatcgt ctggtgccga aaaaagtcga
    19501 tattcgccag cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat
    19561 atgttctggt ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca
    19621 cgcccggttg atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat
    19681 accggtgtgc tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt ggcggcgtcg
    19741 atgatcccgg tgacgcagcg gctgaagttt cttgtcgccc tgcgccccag cgtaacctca
    19801 cctaccgttg ccgctcgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg
    19861 tttaatctgg tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat
    19921 catagcgagc gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg
    19981 ggggaaaccg tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc
    20041 ccgccgattc aacagcctta tcctccgctt tactttggcg gatcgtcaga cgtcgcccag
    20101 gagctggcgg cagaacaagt tgatctctac ctcacctggg gcgaaccgcc agaactagta
    20161 aaagagaaga tcgaacaagt gcgggcgaaa gctgccgcgc atggacgcaa aattcgtttc
    20221 ggtattcgtc tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag
    20281 cggttaatct cgcatcttga tgatgaaact atcgccaaag cacaggccgc attcgcccgg
    20341 acggattccg taggacaaca gcgaatggcg gcgttacata acggcaagcg cgacaatctg
    20401 gagatcagcc ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg
    20461 ctggtgggcg atggtcctac ggtcgctgcg cgaatcaacg aatacgccgc gcttggcatc
    20521 gacagctttg tgctttcggg ctatccgcat ctggaagaag cgtatcgggt cggcgagttg
    20581 ctgttcccac atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg
    20641 caaggcgaag cggtggcgaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaga
    20701 aataatggca acgccagtga agaagtggtt attgcgcgtt gccccctggt ttttaccggt
    20761 gggcatcgtg gcggtgtggc aactggcctc ctcagttggc tggctgtcga cgcgtatttt
    20821 gccctcaccg gaaggggtgg tgacggcgtt ctggacgctc tccgccagcg gcgaactgtg
    20881 gcagcatctg gcgatcagtt cctggcgggc gctgattggc ttttcaattg gcggatcgct
    20941 ggggttgatt ttgggactga ttagcgggct atctcgctgg ggagaacggc tgctggatac
    21001 ctcaattcag atgttgcgca acgtaccgca tctggcgctg atcccgctgg tgattttgtg
    21061 gtttggcatt gatgaatccg caaaaatctt tctggtggcg ctcggcacgc tattccccat
    21121 ttatatcaac acctggcatg gcatccgtaa tatcgatcgc ggtctggtgg aaatggcgcg
    21181 tagctatgga ttatccggca taccgctgtt tatccatgtg atcctgcctg gtgccctgcc
    21241 ttcaattatg gtcggcgtgc gttttgcgtt gggcctgatg tggctgacgc tgattgttgc
    21301 cgaaaccatt tctgccaatt caggcattgg ttatctggcg atgaatgcgc gggagttttt
    21361 gcaaacggac gtggtggtag tcgctattat tctttacgcc ctgctcggca aacttgctga
    21421 cgtcagcgcg cagctgttag agcgcctctg gttgcgctgg aacccggctt atcatttgaa
    21481 ggaggccacg gtatgaatac tgctcgtctg aaccagggca caccattgtt gctcaatgca
    21541 gtaagcaaac attacgcgga aaatatcgtc ctgaaccaac tggatttaca tattccggca
    21601 ggtcagtttg tggcggtggt gggccgcagc ggtggtggca aaagtaccct gctgcgcctg
    21661 ctggcaggtc tggaaacgcc aaccgcaggc gatgtgttag cgggcaccac accgctggct
    21721 gagattcagg aagacacgcg aatgatgttt caggatgcgc gtctgctgcc gtggaaatcg
    21781 gtgattgata acgttgggtt aggccttaaa ggccagtggc gcgatgccgc gcgtcgggcg
    21841 ttggctgcag taggactgga gaatcgcgcc ggggaatggc ctgccgcact ttctggcggg
    21901 cagaaacagc gtgtggcgct ggcaagagcg ttgattcatc gaccgggttt attgttgctt
    21961 gatgaaccgc tcggggcgct ggacgcctta acgcgactcg agatgcagga tttgattgtg
    22021 tcactttggc agcagcacgg ctttaccgta ctgttggtga cgcatgatgt gagcgaagcg
    22081 gtagcaatgg ctgaccgggt gctgttaatt gaagagggaa aaattggtct ggatttgacg
    22141 gtggatatcc ctcgtccgcg ccggttaggg tcggtgcgat tagcggagct ggaagcggaa
    22201 gtattgcaac gggtaatgcg gcgtgggcat tctgaacaac tgattcggcg tcatggttaa
    22261 tatgccggat gtggcgcaca aaaccagcat tttcaacgga ctagtaggcc tgataagaca
    22321 cgtcaagcat cgcatcaggc agttgggcac ggctgccgga tgcggcgtga acgccttatc
    22381 cagcctacaa aaccagcaat ttcaacggag ttgtaggcct gataagacgc gtcaagcgtc
    22441 gcatcaggca ttttggctca aaagtatcaa gccagtgctt tggtgatctt ctcgtacaga
    22501 tcgccagaga gattttccag ccctttcaac tgttccagcg ccgcgcgcat tttctcctga
    22561 cgtttggcat cataacgttt caggcgaatc agcggttcaa tcagacgtga agctacctgc
    22621 gggttacggc tgttgaggtc ggtaagcatt tccaccagga actggtaacc gctgccatct
    22681 tcggcatgga acgctgccgg gttgctgccc gcaaacgcac caatcaacga acgaatacgg
    22741 ttcgggttgc tcatggtaaa tgagcgatgc tgcaacagac cacgcaccgt ctccagcaca
    22801 ttcgccgccg ggctggtggc ttgcaggata aaccatttat ccatcaccag accgtcctga
    22861 tgccacttat cgtcgtactc ctgcatcagc gcgtcacggc aaggcagctg tgcagcaacc
    22921 gccgcagaaa gcgctgccag cgcatcggtc atattgtttg cttcatggta ttgcttgctc
    22981 accagcacgt cagccagatg cgtttcacca aaagcaagga agcgcaggca ggcattacgc
    23041 agagtgcgtt ttgcaatatc ttcatgctca acacggtact cgctctggta attcgcgttg
    23101 taaatagcca gcagctcatc cgccagttca gtcgccagag tacgagtgag tgcttcgcgt
    23161 acttcggcaa tagcaatcgg gtcgatgata tcgaacagtt cagccatttc attgacagaa
    23221 ggcagcgtca ggatttctgc cgccagcgcc ggatcaatct tctcatcgag cagcaccgcg
    23281 cggaaagcgt cagccacatg taccggcaga gacagcggct gcccttgctg atgacgggcg
    23341 acgttcagct tgatgtaggt tgccagcaaa ctttgcgccg catcccaacg ggagaaatca
    23401 ttacgcgcat gacgcatcag gaaggtcagt tgctgatcgc tccacttata ttccagtttc
    23461 actggcgcag agaattcgca cagcagcgca ggcaccggct ggaagtagac attatcaaaa
    23521 acaaaggtct gttccgcctg ggtgacgttc agcacggaat tcaccggatg accgcctttc
    23581 tgcaacggga tcactttgcc ttcgttgtca tacaattcga tggcaaatgg aatatgcagc
    23641 ggctgttttt ctgcctgatc cggcgtggct ggcgtgcgct ggctgatggt cagggtgtac
    23701 tgctcggttt ccggattgta gtcgtctttg acggtcacaa tcggcgtgcc ggactggctg
    23761 taccaacggc ggaaatggga gagatcgaca ttcgacgcat cttccatcgc ctgcacaaag
    23821 tcgtcgcagg ttgccgcgct gccatcatgg cgctcgaaat agagttgcat ccctttctgg
    23881 aagttttctt cgcccagcag ggtgtggatc atgcgaatca cttccgcgcc cttctcgtaa
    23941 acggtcaggg tgtagaagtt gttcatctca atgaccatat ccgggcggat cgggtgcgcc
    24001 atcgggctgg cgtcttccgc aaactgcaat ccgcgcatgg tgcgcacatt attgatacgg
    24061 tttaccgcgc gggaaccaag gtcagagctg aattcctgat cgcggaagac ggttaaacct
    24121 tctttcaggc tgagctggaa ccagtcgcgg caggtcactc ggttaccggt ccagttatgg
    24181 aaatattcat ggccgataac gcgttcaata tcgagataat ctttgtcggt ggcggtgtcg
    24241 gtgcgggcca acacatattt ggagttaaag atattcagac ccttattctc cattgcgccc
    24301 atattgaaga aatccaccgc gacgatcata tagatgtcga ggtcatactc caggccaaag
    24361 cgttcttcat cccatttcat ggagtttttc agcgaggtca tcgcccacgg cgcgcgatca
    24421 aggttgccgc gatcgacgta cagctccagt gctacttcgc gaccagaacg cgtggtaaag
    24481 gtatcgcgca gtacatcaaa gtcgcctgcc accagcgcaa acaggtagca cggtttcggg
    24541 aacgggtcct gccactgtac ccaatggcgt ccgttttcga gttcgccttg cgcaacgcgg
    24601 ttaccattgg aaagcaggaa gggatatttg attttatcgg caataatttt ggtggtaaaa
    24661 cgcgccagca cgtccgggcg gtcgagataa tacgtaatat ggcggaaacc ttcggcttca
    24721 cactgggtgc aaagcgcatc gcctgactga taaagccctt ccagcgccgt attcgccgcc
    24781 gggcttattt cattaatgat cttaagcgta aaacgctccg gcaaattact gatgaccagt
    24841 gcgccctctt cttctttcca ggcggtccac ggctcatcat taatatgaac agaaaccagt
    24901 ttgaggtctt cgccgttgag acgaagcgga gcatctgatg caccatgacg gacagcctgg
    24961 ctgaccgcgg taacgaccgt cttttgcgcg tcgaggtcaa aggtcaagtc aatatcagta
    25021 atctggtaat ccggcgcacg ataatcgtga cggtatttgg cttgtggctg ttgagtcata
    25081 aataaccttt agcatctttt atagagtctg gtgttcagtc tattcctgtt gcgtaaatcg
    25141 cgctatgcag aatcttcatc ttttcaggta caaacgcctt tattgctaca tttttataac
    25201 atacaccgcg taatgccatc gaccagaaag gtggcatatg gtgtgatcgg ggttcaataa
    25261 attgcgaaac aaggtatact ccagcagttc ctgaagatgt ttattgtact aaacgctcct
    25321 gtacgaggac gctactgcgc acctatgaca caattcgctt ctcctgttct gcactcgttg
    25381 ctggatacag atgcttataa gttgcatatg cagcaagccg tgtttcatca ctattacgat
    25441 gtgcatgtcg cggcggagtt tcgttgccga ggtgacgatc tgctgggtat ttatgccgat
    25501 gctattcgtg aacagattca ggcgatgcag cacctgcgcc tgcaggatga tgaatatcag
    25561 tggctctctg ctctgccttt ctttaaggcc gactatctta actggttacg cgagttccgc
    25621 tttaacccgg aacaagtcac cgtatccaac gataatggca agctggatat tcgcttaagc
    25681 ggtccgtggc gtgaagtcat cctctgggaa gttcctttgc tggcggttat cagtgaaatg
    25741 gtacatcgct atcgctcacc gcaggccgac gttgcgcaag ccctcgacac gctggaaagc
    25801 aaattagtcg acttctcggc gttaaccgcc ggccttgata tgtcgcgctt ccatcttatg
    25861 gattttggca cccgtcgccg tttttctcgc gaagtacaag aaaccatcgt taagcgtctg
    25921 caacaggaat cctggttcgt gggcaccagc aactacgatc tggcgcgtcg gctttccctc
    25981 acgccgatgg gaacacaggc acacgaatgg ttccaggcac atcagcaaat cagcccggat
    26041 ctagccaaca gccagcgagc tgcacttgct gcctggctgg aagaatatcc cgaccaactt
    26101 ggcattgcat taaccgactg catcactatg gatgctttcc tgcgtgattt cggtgtcgag
    26161 ttcgccagtc gctatcaggg cctgcgtcat gactctggcg acccggttga atggggtgaa
    26221 aaagccattg cacattatga aaagctggga attgatccac agagtaaaac tctggttttc
    26281 tctgacaatc tggatttacg caaagcggtt gagctatacc gccacttctc ttcccgcgtg
    26341 caattaagtt ttggtattgg gactcgcctg acctgcgata tcccccaggt aaaacccctg
    26401 aatattgtga ttaagttggt tgagtgtaac ggtaaaccgg tggcgaaact ttctgacagc
    26461 cctggcaaaa ctatctgcca cgataaagcg tttgttcggg cgctgcgcaa agcgttcgac
    26521 cttccgcata ttaaaaaagc cagttaatat catcagggag ctaatcggct cccttttttt
    26581 acctttaatt ccgaaatctt tcgctgcatt tgcgaattct gcttgtctga ttgcagatgc
    26641 caggtaacat aggtatcccc ccatttacgg gtggacaagt gtttattttt tccgactatt
    26701 aacagagaga atattatgag cgttgtgcct gtagccgacg tactccaggg ccgcgtagcc
    26761 gttgacagcg aagtcaccgt acgcggatgg gtacgtaccc gccgagattc aaaagctggc
    26821 atctccttcc tcgccgttta tgacggttcc tgctttgatc ctgtacaggc tgtcatcaat
    26881 aattctctgc ccaattacaa tgaagacgtc ctgcgtctga ccaccggctg ctcggtcatt
    26941 gtgacgggta aagtcgtggc gtcgccgggc caggggcaac aatttgaaat tcaggccagc
    27001 aaagttgaag ttgctggttg ggttgaagat ccagacactt acccgatggc ggcaaaacgc
    27061 cacagcattg agtatctgcg tgaagtcgct cacctgcgtc cgcgcacaaa cctgattggt
    27121 gccgtcgcgc gcgttcgcca tacgctggcg caggcgctgc atcgcttctt taacgagcag
    27181 ggattcttct gggtttcaac gccactgatt accgcatctg ataccgaagg tgcaggcgaa
    27241 atgttccgcg tttctacgct ggatctggaa aacctgccgc gtaacgatca gggcaaagtg
    27301 gatttcgaca aagacttctt tggtaaagag tctttcctga ccgtatctgg ccagttgaac
    27361 ggcgaaacct acgcttgcgc attatccaaa atttatacct tcggcccgac tttccgtgct
    27421 gaaaactcca acaccagccg tcacctggcg gaattctgga tgctggagcc ggaagtggcg
    27481 tttgctaacc tgaacgatat cgcgggtctg gctgaagcca tgctgaaata tgtcttcaaa
    27541 gcggttctcg aagaacgcgc agacgacatg aaattcttcg ctgaacgcgt agataaagat
    27601 gccgtttcac gtctggaacg cttcattgag gccgatttcg cgcaggtgga ttacaccgac
    27661 gcagtgacca ttctcgaaaa ctgcggcagg aagtttgaaa acccggttta ctggggcgtc
    27721 gatctctctt ctgagcatga gcgttatctg gcggaagaac actttaaagc accggtagtg
    27781 gttaaaaact atccgaaaga tattaaagcg ttctatatgc gcctcaacga agacggtaaa
    27841 accgttgcgg ctatggacgt tctggctccg ggcatcggtg agatcattgg tggctcccag
    27901 cgtgaagaac gtctggacgt tctggacgag cgtatgctgg aaatgggcct gaataaagaa
    27961 gattactggt ggtatcgcga tctgcgtcgc tacggtactg ttccgcattc aggtttcggt
    28021 cttggttttg aacgcctgat tgcttacgta actggcgtgc aaaacgtgcg tgatgtgatt
    28081 ccgttcccac gtactccgcg caacgccagc ttctgatttt cttcctcaaa gagccagcgc
    28141 ctgctggctc ttttttatcc tttctgtcaa gttatctgtt tgttaagtca agcaatctat
    28201 ttgcaacccc gccataacga tcatcctgtt acggaatatt acattgcaac atttacgcac
    28261 aaaaaataac ccgcattctt attgcggatt agttttttct tagctgatag cacaattttc
    28321 atactatttt ttggcattct ggatgtctga aagaggattt tgtgccaggt cgataaagtt
    28381 tccatcagaa acaaaatttc cgtttagtta atttaaatat aagtaaatca tataaataga
    28441 taaaaattgc tgtaa
//
LOCUS       DAZGSP010000066        27943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZGSP010000066 DAZGSP010000000
VERSION     DAZGSP010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.066"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            22..345
                     /locus_tag="ACHK74_003096"
     CDS             22..345
                     /locus_tag="ACHK74_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFP0221866.1"
                     /translation="MKWIVIDTIIQPSCGISFSAIWGNMKMIIWYQSTIFLPPGSIFT
                     PVKSGIILKDKEYPITIYNIAPFNKDLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(448..1698)
                     /gene="icd"
                     /locus_tag="ACHK74_003097"
     CDS             complement(448..1698)
                     /gene="icd"
                     /locus_tag="ACHK74_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFP0221867.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1870..2523
                     /gene="rluE"
                     /locus_tag="ACHK74_003098"
     CDS             1870..2523
                     /gene="rluE"
                     /locus_tag="ACHK74_003098"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFP0221868.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2533..2994
                     /gene="nudJ"
                     /locus_tag="ACHK74_003099"
     CDS             2533..2994
                     /gene="nudJ"
                     /locus_tag="ACHK74_003099"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFP0221869.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            3048..4154
                     /gene="mnmA"
                     /locus_tag="ACHK74_003100"
                     /gene_synonym="trmU"
     CDS             3048..4154
                     /gene="mnmA"
                     /locus_tag="ACHK74_003100"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFP0221870.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            4190..4831
                     /gene="hflD"
                     /locus_tag="ACHK74_003101"
     CDS             4190..4831
                     /gene="hflD"
                     /locus_tag="ACHK74_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFP0221871.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4835..6205
                     /gene="purB"
                     /locus_tag="ACHK74_003102"
     CDS             4835..6205
                     /gene="purB"
                     /locus_tag="ACHK74_003102"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFP0221872.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAVVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6373..7044
                     /gene="phoP"
                     /locus_tag="ACHK74_003103"
     CDS             6373..7044
                     /gene="phoP"
                     /locus_tag="ACHK74_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFP0221873.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            7044..8504
                     /gene="phoQ"
                     /locus_tag="ACHK74_003104"
     CDS             7044..8504
                     /gene="phoQ"
                     /locus_tag="ACHK74_003104"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFP0221874.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLANWANNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8580..9701
                     /gene="roxA"
                     /locus_tag="ACHK74_003105"
                     /gene_synonym="ycfD"
     CDS             8580..9701
                     /gene="roxA"
                     /locus_tag="ACHK74_003105"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFP0221875.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9847..11076)
                     /gene="pepT"
                     /locus_tag="ACHK74_003106"
     CDS             complement(9847..11076)
                     /gene="pepT"
                     /locus_tag="ACHK74_003106"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFP0221876.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGNIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            11326..12462
                     /gene="potA"
                     /locus_tag="ACHK74_003107"
     CDS             11326..12462
                     /gene="potA"
                     /locus_tag="ACHK74_003107"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFP0221877.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12446..13303
                     /gene="potB"
                     /locus_tag="ACHK74_003108"
     CDS             12446..13303
                     /gene="potB"
                     /locus_tag="ACHK74_003108"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFP0221878.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            13300..14094
                     /gene="potC"
                     /locus_tag="ACHK74_003109"
     CDS             13300..14094
                     /gene="potC"
                     /locus_tag="ACHK74_003109"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFP0221879.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            14091..15137
                     /gene="potD"
                     /locus_tag="ACHK74_003110"
     CDS             14091..15137
                     /gene="potD"
                     /locus_tag="ACHK74_003110"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFP0221880.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            15195..15656
                     /locus_tag="ACHK74_003111"
     CDS             15195..15656
                     /locus_tag="ACHK74_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFP0221881.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            15653..16441
                     /locus_tag="ACHK74_003112"
     CDS             15653..16441
                     /locus_tag="ACHK74_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP0221882.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(16580..17401)
                     /gene="cobB"
                     /locus_tag="ACHK74_003113"
     CDS             complement(16580..17401)
                     /gene="cobB"
                     /locus_tag="ACHK74_003113"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFP0221883.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(17417..18328)
                     /gene="nagK"
                     /locus_tag="ACHK74_003114"
     CDS             complement(17417..18328)
                     /gene="nagK"
                     /locus_tag="ACHK74_003114"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFP0221884.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(18357..19601)
                     /gene="lolE"
                     /locus_tag="ACHK74_003115"
     CDS             complement(18357..19601)
                     /gene="lolE"
                     /locus_tag="ACHK74_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFP0221885.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGVNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(19601..20302)
                     /gene="lolD"
                     /locus_tag="ACHK74_003116"
     CDS             complement(19601..20302)
                     /gene="lolD"
                     /locus_tag="ACHK74_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFP0221886.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(20295..21494)
                     /gene="lolC"
                     /locus_tag="ACHK74_003117"
     CDS             complement(20295..21494)
                     /gene="lolC"
                     /locus_tag="ACHK74_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFP0221887.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            21756..22829
                     /locus_tag="ACHK74_003118"
     CDS             21756..22829
                     /locus_tag="ACHK74_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP0221888.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLAEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWSIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQQFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLGYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAISYSR"
     gene            22957..26403
                     /gene="mfd"
                     /locus_tag="ACHK74_003119"
     CDS             22957..26403
                     /gene="mfd"
                     /locus_tag="ACHK74_003119"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFP0221889.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARILLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            26547..27509
                     /gene="ldtC"
                     /locus_tag="ACHK74_003120"
     CDS             26547..27509
                     /gene="ldtC"
                     /locus_tag="ACHK74_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFP0221890.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(27592..27849)
                     /gene="bhsA"
                     /locus_tag="ACHK74_003121"
                     /gene_synonym="ycfR"
     CDS             complement(27592..27849)
                     /gene="bhsA"
                     /locus_tag="ACHK74_003121"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFP0221891.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6858 a         7204 c         7049 g         6832 t
ORIGIN      
        1 ccgtgggata agacataaca aatgaagtgg atagtaattg acacgataat tcaaccttca
       61 tgcggcatat ctttttcagc catatggggt aatatgaaaa tgatcatctg gtatcaatct
      121 actatatttc tccctcctgg cagtatattt acaccggtta aatctggaat tatccttaaa
      181 gataaagaat atcctattac tatttataac atcgcaccat tcaacaagga tttatggagt
      241 ctactcaaaa gcagtcaaga gtgtcctcca ggagaaagca aaataacaaa taaatgtttg
      301 cataatagtt gcattataaa aatatgccca tatgggctca agtaaagggt tttatataaa
      361 tatgctcgat atgctattaa taatacctac ataaataaaa caacgggcgt gttatacgcc
      421 cgttgcaata ttaaacacat gtagtgatta catgttcttg atgatcgcat caccaaattc
      481 tgaacatttc agcagtttag cgccttccat cagacgctcg aagtcatagg ttacggtctt
      541 cgcattgatt gcgccttcca tacctttaac aatcaggtct gcggcttcag tccagcccat
      601 atggcgtaac atcatctcag cggagagaat aatagagcca gggtttactt tgtcctgacc
      661 ggcgtatttc ggcgcagtac cgtgggtggc ttcaaacagg gcgcattcgt caccgatgtt
      721 tgctccaggg gcgataccga taccgccaac ctgcgctgcc agagcgtcag aaatgtagtc
      781 accgttcagg ttcatacagg cgataacatc atattcagcc ggacgcagca ggatctgttg
      841 caggaatgca tcagcaatca cgtctttaat gacgatctct ttgccggtgt tcgggttttt
      901 aactttcagc cacgggccgc cgtcgatcag ttcaccgcca aactcttcac gcgccagctg
      961 gtagccccag tctttaaacg cgccttcggt gaacttcatg atgttgcctt tgtgcaccag
     1021 agtcacagag tcacgatcgt tagcaattgc gtattcgatc gctgcacgaa ccagacgttt
     1081 ggtgccttct tcagaacacg gcttgatacc gataccgcaa tgttccggga agcgaatttt
     1141 cttcacgccc atctcttcac gcaggaattt aatcactttc tcggcgtcgg cagagtctgc
     1201 tttccattcg atacccgcat aaatgtcttc cgagttttca cggaagataa ccatatcggt
     1261 cagttcaggg tgtttaaccg ggcttggagt gccttgatag taacgtaccg gacgcaggca
     1321 gatgtagaga tccagttcct ggcgcagggc aacgttcaga gagcgaatac cgccaccaac
     1381 aggagtggtc agcggacctt taatggcaac gcgatattca cgaatcagat caagggtttc
     1441 agcaggcagc cagacatcct gaccataaac ctgtgtggat ttttcaccgg tgtaaatttc
     1501 catccaggag attttacgct cgcctttata ggctttctcg actgcagcgt cgaccacttt
     1561 cagcatggct ggggttacat ctacaccgat tccatcacct tcaatgtaag ggataatcgg
     1621 attttcagga acgttgagtt tgccgttttg cagggtgatc ttcttgcctt gtgccggaac
     1681 aactacttta ctttccattc acctctcctt cgagcgctac tggtttgctc gtctgccacc
     1741 acgttgcata tgcgtttgcg tcctgcgata cggatgcttt agagcaattt tttgttaatg
     1801 atttgtaatt ggcttgtcaa tactacccta ttgtttgcag tcatgaaaga tgcgcgttat
     1861 taggctataa tgcggcaatt cataatctct gaaaatacca tgcaaaaaac ttcttttaga
     1921 aatcaccagg ttaagcgatt cagctcgcaa cgttctacca ggcgtaaacc tgaaaaccag
     1981 cccacgcgtg tgatcctgtt caataaaccc tacgatgttc ttccgcagtt caccgatgaa
     2041 gccggacgca aaacattaaa agaattcatc ccggttcagg gcgtttatgc agcaggtcgt
     2101 cttgaccgcg atagcgaagg gttactggtg ctgaccaata acggcgcgtt gcaggcgcgt
     2161 ttaacccagc cgggtaaacg caccgggaaa atctattatg tgcaggtgga aggtattccc
     2221 acacaagacg cacttgaagc cttgcgcaat ggcgtaacct taaatgatgg ccctaccctg
     2281 cccgccggcg cggagctggt tgacgaaccc gcgtggttat ggccgcggaa tccaccaatt
     2341 cgtgaacgca aaagtattcc caccagctgg ctgaagatca ccttatatga aggacgtaat
     2401 cgccaggtgc gccgcatgac cgcccatgtt ggtttcccca cgctgcgact gattcgctat
     2461 gcgatgggag attactcttt ggataatctt gccaatggcg aatggcgaga agtgacagat
     2521 taaggatgca aaatgtttaa accgcacgtt accgttgctt gcgtggtgca cgcagaaggc
     2581 aaatttttag tcgttgaaga gacgattaat ggtaaagcgt tatggaacca acctgccggg
     2641 catctggaag ccgatgaaac cttagtggaa gccgccgccc gtgaactgtg ggaagaaacc
     2701 ggcatcagcg cgcagccgca acactttatt cgtatgcatc agtggattgc gccagataaa
     2761 acgccgtttt tgcgtttcct ctttgccatt gagcttgagc aaatatgccc aacccagcct
     2821 catgacagcg atatcgactg ctgtcgttgg gtcagcgccg aagagatttt aaaggcgtca
     2881 aatcttcgtt cgccgctggt ggcggaaagt atccgttgtt atcaaagcgg gcaacgctat
     2941 ccgctggaga tgattggcga ttttaactgg ccttttacaa agggtgtcat ctaaaaggct
     3001 gcgtgataga atacgccgcc ttgaagttca atgtcgtgag tgatccaatg tctgaaaccg
     3061 caaaaaaagt aatcgtcggc atgtccggcg gtgtcgattc ctccgtttct gcctggctgt
     3121 tgcaacaaca gggatatcag gttgaaggcc tgtttatgaa gaactgggaa gaagacgacg
     3181 gtgaggaata ttgcacagcg gcagcggatc tggctgatgc ccaggctgtc tgcgacaagc
     3241 tcggcattga actgcacacc gttaactttg ctgccgagta ctgggacaac gtcttcgaac
     3301 tgttccttgc cgaatataaa gccggtcgca cgccgaatcc ggatattctg tgcaacaaag
     3361 agatcaaatt taaagccttc ctcgaatttg ccgccgaaga tttaggtgcc gattatatcg
     3421 ctaccggtca ttacgtacgt cgggccgatg tcgatggcaa gagccgcctg ctgcgtggtc
     3481 tggacagcaa taaagaccag agctacttcc tttatacgct cagccatgag cagattgcgc
     3541 aaagcctgtt cccggtcggc gaactggaaa aaccgcaggt gcgtaagatt gctgaagatc
     3601 ttggtctggt caccgcgaag aaaaaagact ctaccggcat ttgcttcatt ggcgaacgta
     3661 aattccgcga gttcctgggc cgttatctcc cggcgcaacc gggcaaaatc attaccgtcg
     3721 atggcgatga aattggtgag caccaggggc tgatgtatca cactctcggt cagcgtaaag
     3781 gtctgggtat cggtggcacc aaagaaggta ccgaagaacc gtggtatgtg gtggacaaag
     3841 acgtcgaaaa caacattctg gttgtcgctc agggccatga acacccgcgg ctgatgtctg
     3901 tcgggttgat tgcccagcag ttgcactggg tcgatcgcga accattcacc ggcactatgc
     3961 gttgcacggt aaaaacccgc tatcgccaga ccgatatccc ttgcaccgtc aaggcgctgg
     4021 acgatgatcg cattgaagtg attttcgatg aaccggttgc cgccgtgacg ccgggccagt
     4081 ctgccgtctt ctataacggt gaagtgtgcc tcggtggcgg tattattgag cagcgtctgc
     4141 cgctgccggt ctgattatta tctttactta aaacaaggaa gcagtgaacg tggcaaagaa
     4201 ttactatgac atcaccctcg ccctggccgg tatttgtcag tcggcacgcc tggtgcaaca
     4261 actcgctcac caggggcatt gtgatgccga tgcgctacac gtctcactca acagtattat
     4321 tgatatgaac cccagctcga cgctggcggt ttttggcggt agcgaagcca acctgcgcgt
     4381 cgggctggaa accctgctcg gcgtgctcaa tgccagcagt cgccagggct taaacgccga
     4441 attaacccgc tacacactca gcttgatggt gcttgagcgc aaactctcct cagcgaaagg
     4501 cgcgctcgac actctgggca accggatcaa cggcctgcaa cgccagctcg aacacttcga
     4561 tttacagtcc gaaacgctga tgagcgcgat ggctgctatc tatgttgatg tgatcagccc
     4621 gcttggcccg cgcattcagg tcaccggttc ccctgctgta ctgcaaagcc cacaagtgca
     4681 ggcgaaagtt cgcgcaaccc tgctggcagg cattcgcgcc gccgtgctct ggcaccaggt
     4741 cggcggcgga cgtctgcaac tgatgttttc tcgtaatcgc ctgaccactc aggcaaaaca
     4801 aattcttgct catttaaccc cggagttgtg atctatggaa ttatcctcac tgaccgccgt
     4861 ttcccctgtc gatggacgct acggcgataa agtcagcgcg ctgcgcggga ttttcagcga
     4921 atatggtttg ctgaaattcc gtgtacaagt tgaagtacgt tggctgcaaa aactggccgc
     4981 gcacgcagcg atcaaggaag ttcctgcttt tgctgccgac gcaatcggtt accttgatgc
     5041 agttgtcgcc aatttcagcg aagaagatgc cgcacgcatc aaaaccatcg agcgtaccac
     5101 taaccacgac gttaaagcgg ttgagtattt cctgaaagaa aaagtggcgg agatcccgga
     5161 actgcacgcg gtttctgaat tcatccactt tgcctgtact tcggaagata tcaataacct
     5221 ctcccacgca ttaatgctga aaaccgcgcg tgatgaagtg atcctgccgt actggcgtca
     5281 actgattgat ggcattaaag atctcgccgt tcagtaccgc gatatcccgc tgctgtctcg
     5341 tacccacggt cagccagcca cgccgtcaac catcggtaaa gagatggcaa acgtcgccta
     5401 ccgtatggag cgccagtacc gccagcttaa tcaggtggag atcctcggca aaatcaacgg
     5461 cgcggtcggt aactataacg cccatatcgc cgcttacccg gaagttgact ggcatcagtt
     5521 cagcgaagag ttcgtcacct cgctgggtat tcagtggaac ccgtacacca cccagatcga
     5581 accgcacgac tacattgccg aattgtttga ttgcgttgcg cgcttcaaca ccattctgat
     5641 cgactttgac cgtgacgtct ggggttatat cgcccttaac cacttcaaac agaaaaccat
     5701 tgctggtgag attggttctt ccaccatgcc gcataaagtt aacccgattg acttcgaaaa
     5761 ctccgaaggg aatctgggcc tttccaacgc ggtattgcag catctggcaa gcaaactgcc
     5821 ggtttcccgc tggcagcgtg acctgaccga ctccaccgtg ctgcgtaacc tcggcgtggg
     5881 tatcggttat gcgctgattg cgtatcaatc caccctgaaa ggcgtgagca aactggaagt
     5941 aaaccgtgac catctgctgg atgagctgga tcacaactgg gaagtgctgg ctgagccaat
     6001 ccagacagtt atgcgtcgct atggcatcga aaaaccgtac gagaagctga aagagctgac
     6061 tcgcggtaag cgcgttgacg ccgaaggcat gaagcagttt atcgatggtc tggcgttgcc
     6121 agaagaagag aaagcccgcc tgaaagcgat gacgccggct aactatattg gtcgagctat
     6181 caccatggtt gatgagctga aataaactcg tatcagtgcc ggatggcgat gctgtccggc
     6241 ctgtttatta aaattatcca ctttttcttt tttcacttta cctcccctcc ccgctggttt
     6301 atttaatgtt tacccccata aacacataat cgcgttacac tattttaata attaagacag
     6361 ggagaaataa aaatgcgcgt actggttgtt gaagacaatg cgttgttacg tcaccacctt
     6421 aaagttcaga ttcaggatgc tggtcatcag gtcgatgacg cagaagatgc caaagaagcc
     6481 gattattatc tcaatgaaca tttaccggat attgcgattg tcgatctcgg attgcctgac
     6541 gaggacggtc tgtcactgat tcgccgctgg cgtagcaatg atgtttcact gccgattctg
     6601 gtattaaccg cccgtgaaag ctggcaggac aaagtcgaag tattaagtgc cggtgctgat
     6661 gattacgtga ctaaaccgtt tcatattgaa gaggtgatgg cgcgaatgca ggcattaatg
     6721 cggcgtaata gcggcctggc ttcacaggtc atttcgctac ccccgtttca ggttgatctc
     6781 tctcgccgtg aattatctat taatgacgaa gtgatcaaac tgaccgcgtt cgaatacacc
     6841 attatggaaa cgttgatacg caataatggc aaagtggtga gcaaagattc gttaatgctc
     6901 caactctatc cggatgcgga gctgcgggaa agccatacca ttgatgtact gatgggacgt
     6961 ctgcgcaaaa aaattcaggc acaatatccg caagaagtga ttactaccgt tcgcggccag
     7021 ggctatttgt tcgaattgcg ctgatgaaaa aattactgcg tctttttttc ccgctctcac
     7081 tgcgggtacg ttttctgttg gcaacggctg cggtagtact ggtgctttcg cttgcctacg
     7141 gaatggtcgc gctgatcggt tatagcgtca gtttcgataa aactacgttt cggctgttac
     7201 gtggcgagag caatctgttc tatacccttg cgaactgggc aaacaataag ttgcatgtcg
     7261 agttacccga aaatatcgac aagcaaagcc ccaccatgac gctaatttat gatgagaacg
     7321 ggcagctttt atgggcgcaa cgtgacgtgc cctggctgat gaagatgatc cagcctgact
     7381 ggctgaaatc gaatggtttt catgaaattg aagcggatgt taacgatacc agcctcttgc
     7441 tgagtggaga tcattcgata cagcaacagt tgcaggaagt gcgggaagat gatgacgacg
     7501 cggagatgac ccactcggtg gcagtaaacg tctacccggc aacatcgcgg atgccaaaat
     7561 taaccattgt ggtggtggat accattccgg tggagctaaa aagttcctat atggtctgga
     7621 gctggtttat ctatgtgctc tcagccaatc tgctgttagt gatcccgctg ctgtgggtcg
     7681 ccgcctggtg gagtttacgc cccatcgaag ccctggcaaa agaagtccgc gaactggaag
     7741 aacataaccg cgaattgctc aatccagcca caacgcgaga actgaccagt ctggtacgaa
     7801 acctgaaccg attgttaaaa agtgaacgcg aacgttacga caaataccgt acgacgctca
     7861 ccgacctgac ccatagtctg aaaacgccac tggcggtgct gcaaagtacg ctgcgttctc
     7921 tgcgtagtga aaagatgagc gtcagtgatg ctgagccggt aatgctggag caaatcagcc
     7981 gcatttcaca gcaaattggc tactacctgc atcgtgccag tatgcgcggc gggacattgc
     8041 tcagccgcga gctgcatccg gtcgccccac tgctggacaa tctcacctca gcgctgaaca
     8101 aagtgtatca acgcaaaggg gtcaatatct ctctcgatat ttcgccagag atcagctttg
     8161 tcggtgagca gaacgatttt gtcgaggtga tgggcaacgt gctggataat gcctgtaaat
     8221 attgcctcga gtttgtcgaa atttctgcaa ggcaaaccga cgagcatctc tatattgtgg
     8281 tcgaggatga tggccccggt attccattaa gcaagcgaga ggtcattttc gaccgtggtc
     8341 aacgggttga tactttacgc cctgggcaag gtgtagggct ggcggtagcc cgcgaaatca
     8401 ccgagcaata tgagggtaaa atcgtcgccg gagagagcat gctgggcggt gcgcggatgg
     8461 aggtgatttt tggtcgccag cattctgcgc cgaaagatga ataaatatgt ccatacttca
     8521 cgcattacgt taagcatccg ttataatcgg ttgcagatac cagcctgtgg atgcttaaca
     8581 tggaatacca actcactctt aactggcccg attttcttga acgtcactgg cagaaacgcc
     8641 cggtggtgtt aaaacgcggc tttaataatt ttattgaccc gatctctcca gacgagttgg
     8701 cgggtctggc gatggaaagc gaagttgaca gtcgactggt cagtcaccag gatggcaaat
     8761 ggcaggtcag ccacggcccg ttcgaaagct acgatcatct cggtgaaacc aactggtcat
     8821 tactggtaca ggcagtgaac cactggcatg agccgaccgc cgcgctgatg cgaccgttcc
     8881 gtgaactacc ggactggcgt attgatgatc tgatgatctc tttttctgta ccgggcggcg
     8941 gcgtcggccc gcatctcgat cagtacgacg tgtttatcat tcagggtacc ggtcgtcgtc
     9001 gctggcgagt gggcgaaaag ctgcaaatga aacagcactg tccgcacccg gatctgttac
     9061 aggtcgatcc gttcgaagcc atcatcgatg aagagctgga gcctggcgat attctttata
     9121 ttccgccagg attcccgcat gaaggctacg cgctggaaaa tgcgatgaac tattccgtgg
     9181 gttttcgcgc gccgaatacg cgggaactga ttagcggatt tgccgattat gtgctgcaac
     9241 gtgaactggg cggcaactac tacagcgatc cggatgttcc acctcgcgct catcctgcgg
     9301 atgttctgcc gcaagagatg gataaactgc gtgagatgat gctcgaattg atcaaccagc
     9361 cggaacactt taagcaatgg tttggcgagt ttatatccca gtcacgtcat gaactggata
     9421 tcgcgccgcc ggaaccgcct tatcagccag atgaaatcta cgatgcgctg aaacaaggtg
     9481 atgtgctggt gcgcctgggt ggtctgcgcg tattgcgcat tggcgacgac gtgtatgcca
     9541 atggtgagaa gatcgattcc ccgcaccgtc cggcactgga tgcactcgcc agcaacattg
     9601 cgctgactgc ggagaatttt ggcgatgcgc tggaagatcc gtcattcctc gcaatgctcg
     9661 cggcgctggt aaatagcggg tattggttct tcgaagggta agtttggatt taagccggat
     9721 gtggtgctga ttgcgcctta tcgtgcctac atgcactgaa cgcctgtagg cacgataagt
     9781 atgtggacca gtgtcaatat aacggtttca tcccggtaat tggcagtcca catacgcatc
     9841 gggcaatcac gacttccgtt gcgccgttaa ctcggcaata cggacgatca cctgcaccgc
     9901 tttttccata ccttccagag tcacaaactc atgcttacca tgatagttgt aaccgccagt
     9961 gaacaggttc gggcaaggta atcccataaa cgacaactgc gcgccgtcgg taccgccgcg
    10021 gatcggtttc agttccggtt caatatcgca gtcacgcatc gcctgctggg cgatatcgag
    10081 aatatgcgga tgctcaacca ctttctcgcg catattgtag taactgtctt caatcaccaa
    10141 ttcaatgtag caatcaggat gtaacccttt gcccactttt ttggcgatct ccatcatttt
    10201 acgtttacgc gcttcaaact gtttacggtc gaaatcacgg atgatgtaat gcatatcggc
    10261 ccgttcaacg gtgcctttca tgctcgccag gtgatagaaa ccttcatagc cttctgtcat
    10321 ttccgggctt tcatccgccg gaacttccgc atgaatacgt gccgccagcg acagcgcatt
    10381 taccatcact cctttcgccg tgcccgggtg aacattgtta ccgacaattt tgatattgac
    10441 cgacgcggcg ttgaagtttt caaactccag ttcgcctacg ccaccaccat caacagtgta
    10501 agcccagcgg gcatcgaagg cgtcaacatc aaaatgtttc gcccctttgc ccacttcttc
    10561 atccggggta aaggcgacgc gaatatcacc atgcggaatg tttttctgtt gcaataccgc
    10621 cagcgcggtc atgatttctg caataccggc tttgtcatcg gcacctaaca aggttttacc
    10681 atcggtagta atcagcgtct gacccagtag ctgatgcagc accgggaaca taaccggtga
    10741 taaaacttca tcgccgatac ccagcgcaat atcgccacct cgatagtttt caacaatttg
    10801 cggattcaca tttttgccgc tgcaatccgg tgaggtatcc acatgagaaa taaagccaat
    10861 cgccgggata ttgccaggga cgttagccgg taacgtcgcc atcaaagtgc ccttctcact
    10921 taaggtcaca ttgataagcc ccatctcttc gagctgctct ttcagcagat gcaataactt
    10981 ccattggcct tccgtgctgg gaacctgtct cacccctgct tttgattggg tatccagaga
    11041 cacgtagttc aaaaatcgct caagtagttt atccatgtag tcaccctcac tttttgtgac
    11101 aacattatta agaagcaaga aaagacaaat attgcgtcag gtcactttta cccctgcaag
    11161 cgggaatatt tatcagcatt accttaatga atataaagct aagccagtaa ttcacaacaa
    11221 agattggcga ttcaagcggt ttgccgtaga attaccgccc tcattaagag tcaccaaggt
    11281 ggttaaccac aaaccccgca tcggtaagcc atccgttgcg tttacatggg acagagtaaa
    11341 aaattgaata aacaaccgag ttcgctttca ccgctggtgc aattggcggg aattcgcaaa
    11401 tgctttgatg gtaaagaggt cattccccag ctggatctga ctatcaacaa tggcgagttc
    11461 ctcacgctgc ttggcccttc tggctgcggt aaaacaaccg ttcttcgcct gattgcaggt
    11521 ctggaaactg ttgattccgg acgcatcatg ctggataacg aggacatcac ccacgttccg
    11581 gcggaaaacc gctatgtgaa cactgttttc caaagctacg cacttttccc ccacatgacc
    11641 gtgttcgaaa atgtggcctt tgggttgcgc atgcaaaaaa cccccgctgc tgaaattacg
    11701 ccccgcgtga tggaagccct gcggatggtg cagctggaaa ccttcgctca acgcaaaccg
    11761 catcagctct ctggtggtca acagcaacgc gtcgccattg cccgcgcggt ggttaacaag
    11821 cctcgtctgt tgttgctgga tgagtcgctc tcagcgctgg attacaaact gcgcaagcaa
    11881 atgcagaacg agctgaaagc gttacagcgt aagcttggca ttacattcgt cttcgtgact
    11941 cacgaccagg aagaagcact caccatgtca gacaggattg tggtgatgcg cgatggtcgc
    12001 attgagcaag acggcacgcc gcgtgaaatc tacgaagagc cgaaaaacct gtttgttgcc
    12061 gggttcattg gcgaaattaa tatgtttaac gccacggtga tcgagcgact cgacgagcag
    12121 cgcgtacgcg ccaacgttga aggccgcgaa tgtaatatct acgttaactt cgccgttgaa
    12181 ccggggcaaa aactgcatgt tctgctgcgc ccggaagact tacgtgttga agagattaac
    12241 gacgacaacc acgctgaagg gctgattggt tacgttcgtg agcgtaacta caaaggcatg
    12301 acgctggagt cggttgttga actggaaaat ggcaagatgg tgatggtcag cgaattcttc
    12361 aatgaagacg atcctgactt tgaccactct ctcgaccaaa aaatggccat taattgggta
    12421 gaaagctggg aggtcgtact ggctgatgaa gaacacaagt aagttccaga atgtagtgat
    12481 tgtcactatt gtcggttggc ttgtgttgtt tgtctttctg cccaacctga tgatcattgg
    12541 caccagcttt ttgacccgcg acgacgccag tttcgtcaaa atggtcttta cgctggataa
    12601 ctacacgcgt ctgctcgatc cgctctattt tgaagtgcta ttgcactcgc tgaatatggc
    12661 gctgatcgcc accctcgcct gcctggtgct gggctatcca tttgcctggt ttctggcgaa
    12721 gctgccacac aaggtgcgtc cgctgctgct gtttctgctg attgttccgt tctggaccaa
    12781 ctcattgatt cgtatctacg ggctgaaaat tttcctcagc accaaaggct atctcaacga
    12841 gtttttgctc tggctgggcg ttatcgacac accaattcgt atcatgttca cgcccagtgc
    12901 ggtgattatc ggtctggttt acattctgct accgtttatg gtgatgccgc tgtactccag
    12961 tatcgaaaaa ctggataagc cgttgcttga ggcggcgcgc gatctcggtg ccagcaagtt
    13021 acagactttt atccgtatca ttattccact gacgatgccg ggaattattg ccggatgtct
    13081 gctggtgatg ctgccagcga tgggcctgtt ctatgtatcc gacctgatgg gcggtgcgaa
    13141 aaacctgctg atcggtaacg tcatcaaggt acagttcctc aatattcgtg actggccatt
    13201 tggtgcagcc accagcatta cgctgactat cgtaatgggc ctgatgttgc tggtttactg
    13261 gcgcgcttct cgtctgctga ataaaaaggt ggaactcgaa tgatcggtcg actgcttcgc
    13321 ggcggtttta tgaccgctat ctacgcgtac ctgtatatcc caatcattat tttgattgtg
    13381 aactccttta acagctcgcg ctttggcatc aactggcagg gttttaccac caaatggtat
    13441 agcctgctga tgaacaacga cagcctgtta caggcagcgc agcattcact aacaatggcg
    13501 gtgttttcgg cgacgtttgc tacgcttatc ggttcactga cggcagttgc gctgtaccgt
    13561 tatcgctttc gcggtaagcc gttcgttagc ggaatgctgt ttgtggtgat gatgtcgcca
    13621 gatattgtga tggcgatttc tctgctggtg ctgtttatgc tgctgggtat tcagcttggc
    13681 ttctggtcgc tgctgttctc gcatatcacc ttctgcctgc cgtttgtggt ggtgacggtg
    13741 tattcgcgcc tgaaaggttt tgacgtgcgg atgctggaag cggcgaaaga tctcggtgcc
    13801 agtgaattta ccattctgcg aaaaatcatt ctgccactgg caatgcctgc ggtggcggcg
    13861 ggctgggtgt taagctttac cctgtcgatg gacgatgtag tagtttcttc gttcgtcacc
    13921 ggaccaagct atgaaattct gccgttaaaa atttattcga tggtcaaagt cggcgtatcg
    13981 ccggaagtta acgcgctggc aaccatatta ctggtgctgt cgctggtgat ggtaattgcc
    14041 agccagctta ttgctcgtga taaaacgaaa ggtaacacag gggacgttaa atgaaaaaat
    14101 ggtcacgcca cctgctcgcg gcgggtgctc tggcactggg catgagcgcc gctcacgccg
    14161 atgacaacaa tacgctgtat ttctacaact ggaccgagta cgtgccgcca ggactgcttg
    14221 aacagttcac caaagaaacc ggtattaagg ttatctattc gacttacgag tcgaacgaaa
    14281 ccatgtacgc caagttgaaa acgtacaaag acggtgccta cgacctggtg gttccttcaa
    14341 cctattacgt cgataaaatg cgtaaagaag ggatgatcca gaagatcgac aagtcgaagt
    14401 taagcaattt cagcaacctc gatccagaca tgctcaacaa gccgtttgac ccgaataacg
    14461 actactccat tccgtatatc tggggggcga cggcgattgg cgttaacggt gatgcggtag
    14521 atccgaaatc cgttaccagc tgggccgatc tgtggaagcc tgagtacaaa ggtagcctgc
    14581 tgctgaccga cgatgcccgt gaagtgttcc agatggcgct gcgcaaactg ggctactccg
    14641 gtaacactac cgatccgaaa gagattgaag ctgcatataa cgagctgaaa aaactgatgc
    14701 caaacgtcgc agcgtttaac tccgataacc cggctaaccc gtacatggaa ggcgaagtta
    14761 acctcggcat gatctggaac ggctctgctt tcgttgcacg ccaggcgggt acgccaattg
    14821 acgtggtgtg gccgaaagaa ggcggcattt tctggatgga cagcctggcg atcccggcaa
    14881 atgccaaaaa caaagaaggc gcgctgaaat tgatcaactt cctgctgcgc ccggatgtgg
    14941 caaaacaggt tgccgaaact atcggttatc caacgccaaa ccttgcggcg cgtaagctgt
    15001 taagcccgga agtggcgaac gataaaacgc tctacccgga tgctgaaacc attaaaaatg
    15061 gtgaatggca gaatgacgtt ggcgcagcca gcagcattta tgaagagtat tatcagaagc
    15121 tgaaagcagg acgttaattc agcaaacggg cggtggtatt accgcccgtt ttttagttag
    15181 caaggatgtg gctaatgagt caggacagta aagttttatc ccgcgctttt ctcggtatcg
    15241 gtctgatatt gctcgttatt tctgcaatca tcttttatta ccattttact tttaccaaaa
    15301 gcgcggttca tacagaagga atcattgttg atgccgtttg gtacaacaat cactcaaacg
    15361 acgtcgatga taatggttcc tggtatccag tggttgcctt tcgccctacg ccagattaca
    15421 cgctaatttt caattcgaat atcggtagtg atttttatga agatagcgaa ggtgacagag
    15481 tcaatgtcta ttatccgcca gggcatccag aacaagccga aataaacaat ccctgggtta
    15541 atttttttaa atggggcttt gttggcatag caggcattat tttcggtagt gtcggtctga
    15601 taatcacgct cccctccacc aaaaaatctc gcaggaaacg gaagtcccgc ccatgaaaaa
    15661 attcattata ctgctcagtt tactgatact cctgcctctg gtagcaacca gtaaaccatt
    15721 gattccaata atgaagacac tttttacgga tgttaccggt actgtaccgg atgctgaaga
    15781 gatcgcacgt aaagccgagc tttttcgcca acaaacggga attgcaccgt ttattgtgat
    15841 tttacccgac attaataatg aagccagtct cagacaaaat ggtaaagcga tgctggcgca
    15901 tgcgtcatct tcattgagta atgtaaaagg aagtgttctg ttactattta ctacccgcga
    15961 accacggtta attatgatca ccaacggcca ggttgaaagt ggtctggacg ataaacatct
    16021 cggccttctg atagaaaatc acacgctggc ttatttaaat gcagatctct ggtatcaggg
    16081 aatcaataat gcattggctg ttctacaagc acagatatta aaacaaccga cgccgccact
    16141 gacgtattat ccgcatccag gacaacagca tgaaaatgct ccccccggca gcaccaacac
    16201 attaggtttt atcgcctggg ctgcgacgtt tatcttattt tcctggatct tttgctacac
    16261 gactcgtttt atttatgcgt taaaatttgc agtggcgatg actattgcca atatgggtta
    16321 tcaggcttta tgtttgtata tcgataatag ctttgcgatt accagaatat cgccactctg
    16381 ggcagggcta attggtgtct gtacgtttat tgccgcattg ttattgacga gtaaacgata
    16441 aaagaggccg gaaaagtgcg cgtgtattat gccggatggg gcgcgaacgc cttatccggc
    16501 ccacaaaacc cgcaaattca attaattgcg tccccttgta ggcctgataa gcgtagcgca
    16561 tcaggcaatg cttcctgctt tataatccct tcagcaactt ttcaacaaac tcaggcacca
    16621 cctggcttgc cgggccgtaa tatttctcgg caaattcatt accgacctga cttggttcga
    16681 gattcagttc cacggtgtgc gcgccatgca gtttcgcttc gtgaacaaac ccagccgccg
    16741 gataaacatg cccggaagta ccaatggcaa tgaaaatatc agccatcgac aacgccatat
    16801 aaatttcatc catgccgagt ggcatttcgc caaaccaaac gacgtgcggg cgcaagggtg
    16861 ccgggaactg gcaacaatgg catttatctt ctggcgtaac gtctccggtc cagtcgagaa
    16921 cctgaccact ttgcgaacaa cgaactttca gcaattcccc atgcatatga atgacattgg
    16981 tattacctgc gcgttcatgc aggttgtcta tattctgcgt taccagcaaa aagcgatcgc
    17041 cgagggcgtc ttgcagttta gccagcgcaa gatgcgcggc gttaggctga atttctggct
    17101 gctgcagctg tcgacgacgg gcgttataaa acgcttgcac cagttcagga tcgcgatcga
    17161 aaccttccgg cgttgccaca tcttcaaccc gatgttcttc ccacaggcca tctgcggcgc
    17221 gaaaggtacg aatacctgat tccgcagaaa tccctgcccc tgtcagtacg agtactcttg
    17281 gtttttccat tgcttccggc accactttat ctctgaaaaa aatacgctga cgcaaacgct
    17341 cgcgcaggcg gcgtttattt ttacgaaaac gacttaaccg atgaccccga cgcgacagca
    17401 tagcaacctc tgttgtttaa tcggttagat gtaggaaggc cgcaccacgc attcctcccg
    17461 catcaccgtg gcgcgcgcgt tcaatgcgcg gaacacgagc tacaggtaag agatgacgag
    17521 gcagcctgtc cgccagttgc gttgtgattg ccgggaaatt cgataagcca ccaccaatga
    17581 cgaccaggtc agggtcaaca atggtcagga tatttcccag acaaaccgct aataaatcca
    17641 gataacgctc aacgtgcgcc cttgcctgct catcgccttg atcataaagc gcaataattt
    17701 cgggagcctg caacggttga tgataatagt gttgatacag ccacgcaaaa ccgcgaccag
    17761 acagataatt ttcaatgcag ccatgctgac cacagccgca gcggcgtaac gggaaatcca
    17821 accccatcat ggttaacgca tcaaccggca gacgcatatg gccaaactcg ccggtaatgt
    17881 agcttttacc ggtaatcggt ttgccgttga aaatcagccc gccgccaacg ccggtgccga
    17941 gaatcaaccc catcaccagt ggatattgag taaattcatc atcccaggct tctgaaaggg
    18001 caaaacagtt ggcatcgtta tcaaggcgta catcgcgatc aagacgtgcg ctcaggtcgg
    18061 cacgcagcgg tttaccgctg gcagcgggga cattggcggc atacagcgta ccatcttctg
    18121 tttccggcat tcccggaata ccgatgccga cagaaccttt acagccaaaa cgttggtcag
    18181 cttcagctac cagctcacac actgcatcta aaaatgcgtc atagctgtca cgcggtgtcg
    18241 gcacccgctt ttcccactgc aactgccgac cgctatcaaa cacgccaagc gcaatttttg
    18301 ttccaccaat atcaaacccg taatacattg ccgctccttg ttttaatgta ctgcctttac
    18361 tggccgctaa ggactcgcgc agggtcaata ttactggcgc gccgcgccgg ataccaactt
    18421 gccaaaagac tcagcaacaa tgctgtgacc agtacgtaga agacgtccag ccagtgcaat
    18481 tccgatggca ggaaatcaat aaaatagata tcgctggaga ggaactgatg accgatcaac
    18541 ttttcaatcc actcaataat cggggtaagt tgcagtgaaa ccaccacacc gataatcacc
    18601 ccacacaggc tgccgaacag ccctgccagc aatccatacc agacaaagat ggcgcgaatt
    18661 aaaccatctt tcgcccccag cgttcttaat actgcgatat cgccactctt gtctttcacc
    18721 gccatcacta atgtggagac gatgttgaaa caggccacgc caatcaccag taccatcgcc
    18781 agatacataa tggcgcggat catctggata tcgcgataca tatagccgta agtaccaatc
    18841 caacttttaa tataaacata gctgttggtc acttcacccg catcgcgtac cagcttattg
    18901 gcgttgaaaa catccgtcat tttaagggca atacctgaca cgctggaacc catatcaaga
    18961 tattgttggg catccgccag cgggatcatg gcaaaactgt gatcgagttg accactcaac
    19021 tgcaaaatac cggcaacgtg caaacgcaca cgttttggct gcatcagttt atgctcggga
    19081 ttcgagttgg ggatcataat cgacacccaa tccccctgct tcactttcag cgcatccgcc
    19141 acgcctttgc cgatgataat ttgctgttcg cccgctttaa aattgcgcca cgcatcgccc
    19201 tggacaaacg agggtaatgc gctcagacgc tgttcctgtt gcgggttaac gcccttcacc
    19261 tggattgcgc gaagattcac gccactttcc accagcccgg tgaaattgat atacggcgcg
    19321 gcagcggcaa tacccggcac tttttgcacg tgatccagtg cttcctgcca gttagtccac
    19381 ggctgatcca ccgcctcgat ttcgccatgc ggcaccaccg ccagaatacg gttgttcagt
    19441 tcgcgttcaa agccgttcat cgcgcttaag ccgacgatca ataccgccac gccgagggca
    19501 atgccaatgg tagaaataac ggagatcagc gacaccatgc cgccgcgccg ccgtccgcga
    19561 ctaaaacgca ggccaattaa taacgataaa ggcatcgcca ttactccgcc cccatcaggc
    19621 tcagttccgc cgtcagacga ccatcacgca tttctaactg gcggctcata cgtttcgcca
    19681 gttgcaggtc gtgagtaacc accaggaagg cggtgccctg caagcgattc aattccccaa
    19741 gcaactggaa gatgctgtcg gcgttacgcg catcgaggtt accggtaggt tcatccgcca
    19801 gtaccaggcg cgggttattc accagcgcac gggcaatcgc cacacgctgg cgttcgccgc
    19861 cagaaagttc agatgggcgg tgattcgcac gatgatccag ccccaccgct tttaacatct
    19921 caagtgcacg gctgttgatt tcagcgggct ttttcttgcc aatcagcagc ggcatagcca
    19981 cgttttccag ggcggtaaaa tccggcagca ggtggtgaaa ctgataaata aagcccagct
    20041 tctggttgcg cagttctgct ttcgccgccg aagacagttt gctcatcggt tgaccgttaa
    20101 agatcacatc gccggaggtt ggtgtatcca gcccgcccag caggtgcagc aaggtacttt
    20161 taccggaacc agagctaccg acgatcgcca tcatttcacc ttcgcccacg ctgaaactga
    20221 cattgtgcag tacatcggtt tgcacactgc cttcctgata gcgtttgcac aggttgtcgc
    20281 attgcaacag gatcttattc ataacgtaaa gcctcagcgg gttgtgtggc ggcagcgcgc
    20341 catgaagggt aaagcgtaga cagcagcgcg atagccatcg ccaccagcgc aataacaatg
    20401 acctgtaaag gttcgatagc caccggcagc gccgcgccat caagcaggac gccgattatc
    20461 ggcatcagat tatttaactg gctggcaagc agtgcgccaa gcgccgcacc gaggatcgca
    20521 ccgataatcc cggcgctggc cccttgcacc ataaagacca tcatgatttg tcgcggagtt
    20581 aagccttgcg tttgcaggat cgcgacttcg ccctgcttct ccattaccat cagccctagt
    20641 gaggtaataa tgttaaacgc cgcaacggcg acaatcaggc tcagcagcaa gcccatcata
    20701 tttttttcca tgcgtacggc ctggaacagc tcgcctttac ggtcacgcca gtcctgccat
    20761 ttgctgcctt caggcagttt ttgctgactt aaagagtcaa ctttcagcgg ctcatccagc
    20821 cacaaacgcc agccggtaat attgcctgcc ggataacgca tcagacgcga ggcatcctca
    20881 atattcacca gcatttcata gccatcgact tcactgttgg cggcgaaagt gccaatcaca
    20941 ttgaacaggc gctggcttgg aatacgcccc atcggcgtga actggctggc agatggtacc
    21001 atcacgcgga tttgatcacc gcgattaacg cctagctgtg aggcaagttg ttcgccgagg
    21061 atgacattat atttccccgg ctcgaggtca gtttgtttca cattgaccag atacggtgta
    21121 agtggatctt tttgcgccgg gtcgataccg agcatcaccc cgaccgccac gctgcgcgcg
    21181 ctttgcagta ccacatcacc ggtagtaata ggtgcgacgc gattaacgcc gtccagtttg
    21241 actgccgttt ctgggagttg ctgcgggtta agagagccat gctcagaaga gagaattgcc
    21301 tgtggcatca ggccaaggat gttgttttgc agctcgcgct caaagccgtt catcactgac
    21361 aatactgtga ccagcgccat caccccgagg gtaatgccga tggtagaaag ccaggagacg
    21421 aaacgaccga agcgatccgc tgcacgccca cgcatgtaac gcaggccaat aaatagagcg
    21481 acaggttggt acatgaaatc cgtctggttg ctgtagcaaa gacacgagta tataagcgaa
    21541 agcaagatgg gtaaacgtct gaactgtaag tgatcgtcag ggctaatctg aaaagccccc
    21601 gtgaatcaat cggctaaatt gccatgtttt atcatcaatc ttgccgcctg atcttaatct
    21661 gaaaaatcat ccaatacaga cagataccca ttacacatag ggtcataacc cgcaggatca
    21721 accgcatgta aattaaaaaa gagcgtgttg tcctgatgaa acaaaaagag ctatggatta
    21781 accagatcaa agggttatgt atttgtctgg tggtgattta tcactcggtc attacctttt
    21841 atccgcatct gaccactttt cagcatccgt tagcggaagt cctgagcaaa tgctggatct
    21901 atttcaatct ttaccttgca ccctttcgta tgccggtttt tttctttatt tccggctatt
    21961 tgattcgccg ctatatcgac agcgtaccgt ggggaaattg tctcgataaa cgcatctgga
    22021 gcatcttttg ggtgctggcg ctttggggcg tggtgcagtg gctggcgtta agtgcgctta
    22081 accagtggct ggcacctgag cgcgatttaa gcaacgcttc caatgccgcg tatgccgatt
    22141 ccaccggtga gttcctgcac gggatgatca cagccagcac cagcttgtgg tatctgtatg
    22201 cgttaattgt ctatttcgtg gtatgtaaaa tttttagccg tctggcgctg ccactattcg
    22261 ccttgtttgt actgctgagt gtggcggtta atttcgttcc cacgccgtgg tggggaatga
    22321 acagtgtgat ccgcaatttg ccttattaca gccttggcgc atggtttggc gcaacattaa
    22381 tgacctgtgt taaagaggtg ccgttgcgcc gccatctgct gatggcttct ttgcttaccg
    22441 ttctggcggt cggtgcctgg ttgtttacta tctcgctgct gttgtcgctg gtatcgattg
    22501 tggtgatcat gaagctgttt tatcaatacg agcaacaatt cggtatgcgc tccaccagcc
    22561 tgctgaatgt gattggctcc aacaccattg ctatctacac cacccatcgc attctggttg
    22621 aaatattcag cttaactctg cttgcgcaaa tgaacgcagc acgctggtcg ccgcaagtcg
    22681 aactgacact cctgctgggt tacccctttg ttagtttgtt catctgtact gttgcgggct
    22741 tgctggtaag aaaactttca cagcgcgcat tcagcgatct gttgttctcc ccgccttctc
    22801 tgcccgcggc catcagttac tcccgctaag ccatcgcccc agccggggcg attcaatttg
    22861 ctaaaccatg tcgattacgg ataatgaaca gcattgctta tcagagatat gcccccatat
    22921 gttgaggcat atcctaacga gaatctgaca accgttatgc ctgaacaata tcgttatacg
    22981 ctgcccgtca aagcgggtga gcagcgtctg ctgggcgagt taaccggcgc tgcctgcgcg
    23041 acgctggtag cggaaattgc cgaacgtcac gccggtccgg tggtactcat tgcaccagat
    23101 atgcaaaatg ctctgcgttt gcatgatgaa atcagccagt ttaccgatca aatggtgatg
    23161 aatctggcgg actgggaaac tcttccctac gacagttttt cgcctcatca ggacattatc
    23221 tcctcgcgcc tttccaccct ttaccagcta ccgacgatgc agcgtggcgt actgattgtt
    23281 ccggtgaata cgcttatgca gcgcgtttgc ccgcacagtt ttcttcacgg tcatgcgctg
    23341 gtgatgaaaa aaggtcagcg tctgtcacga gatgcattaa gaacccaact ggacagcgcc
    23401 ggttatcgcc atgttgacca ggtaatggag cacggcgaat acgccacgcg cggcgcgttg
    23461 ctggatctct tcccgatggg gagtgagctg ccttatcgtc ttgatttctt tgatgatgaa
    23521 atcgacagcc tgcgggtgtt tgacgtcgac agccagcgca cgctggagga agtagaagcg
    23581 atcaatctgc tgcccgcgca cgaatttccg accgataaag cggcaattga actgttccgc
    23641 agccagtggc gcgatacctt cgaagtgaag cgcgatccgg aacatattta ccagcaagtg
    23701 agtaaaggca cattacctgc cgggatcgag tactggcagc cattgttctt cagcgaacca
    23761 ctgccgccgc tgttcagtta tttccctgcc aataccttgc tggtgaatac tggcgatctg
    23821 gaaaccagtg ccgaacgttt ccaggctgac acgctggcgc gttttgagaa tcgcggcgtc
    23881 gatccgatgc gcccgctgtt gccaccacaa tcgctctggc tgcgggtgga cgagctcttc
    23941 tcagagctga aaaactggcc ccgggtgcag ctaaaaactg aacatttacc gacaaaagcc
    24001 gcgaatgcca atttaggttt ccagaaactg ccagacctgg ccgttcaggc acaacaaaaa
    24061 gcgccgctgg atgcgctgcg taagttcctc gagactttcg acggtccggt ggtgttctcg
    24121 gtagaaagtg aaggtcgccg tgaagcgctg ggtgaactgc tcgcacgaat taaaattgct
    24181 ccgcaacgca ttatgcgtct tgatgaagcc agcgaccgtg ggcgttatct gatgattggc
    24241 gctgccgaac atggttttgt cgataaggtg cgtaatctgg cgctgatctg cgaaagcgat
    24301 ctgctcggtg aacgtgttgc ccgccgtcgt caggattctc gccgcaccat caaccccgat
    24361 acactgatcc gtaaccttgc ggagctgcat attggtcagc cggtggtcca tctggagcac
    24421 ggtgtcgggc gctatgccgg aatgaccacg ctcgaagcgg gcggcattac tggcgagtat
    24481 ttgatgctca cctatgccaa cgacgccaaa ctgtatgttc cggtgtcgtc actgcatctg
    24541 attagccgtt acgcaggtgg cgcggaagaa aacgccccgc tgcataaact tggcggcgat
    24601 gcgtggtcac gcgcgcggca gaaagcggcg gaaaaagtgc gtgatgtggc ggcggaattg
    24661 ctggatatct acgcgcaacg cgccgccaaa gagggcttcg cgtttaaaca cgatcgtgag
    24721 cagtatcagt tgttctgcga cagcttcccg tttgaaacca cgccggatca ggcgcaggcc
    24781 attaatgcgg tacttagcga catgtgtcag ccgctggcaa tggatcgtct ggtgtgcggc
    24841 gatgttggct ttggtaaaac agaagtggcg atgcgcgccg ctttcctggc agtagataac
    24901 cacaagcagg tggcggtgtt ggtgcctacc acccttctcg cgcagcagca ttacgacaac
    24961 ttccgcgacc gtttcgccaa ctggccggta cgtatcgaaa tgatctcccg tttccgcagc
    25021 gccaaagagc agacgcaaat ccttgcggaa gtggcggaag ggaaaatcga tattctgatc
    25081 ggtacgcaca aactgctgca aagtgacgtc aagtttaaag atttaggcct gctgattgtc
    25141 gatgaagaac accgcttcgg ggtgcgtcat aaagagcgta ttaaagcgat gcgcgcgaac
    25201 gtggatattc tgacgcttac tgcaacgccg atcccacgta cgctgaatat ggcaatgagc
    25261 ggaatgcgtg acctgtcgat tatcgccacg ccgcccgccc gtcgtctggc agttaaaacc
    25321 tttgtccgtg agtatgacag cctggtggtc cgggaggcga tcctgcgtga aattttgcgc
    25381 ggaggacagg tttattatct ctacaatgat gtggaaaaca ttcagaaagc cgccgaacgg
    25441 ctggcagaac tggtgccaga agcgcggatc gccatcggtc acgggcagat gcgcgagcgc
    25501 gaactggaac gggtgatgaa tgatttccat catcaacgtt tcaacgtgct ggtttgtacc
    25561 accattattg aaaccgggat cgacatcccg acagccaaca ccattatcat tgaacgtgcg
    25621 gatcacttcg gtctggcgca gctgcaccag ttacgcggtc gcgtcggacg ttcgcatcat
    25681 caggcatatg catggttgct gacgccgcat ccaaaagcga tgactaccga tgcacaaaaa
    25741 cgtcttgaag cgattgcctc gctggaagat ctcggggcag gttttgcgct ggcaacgcac
    25801 gatctggaga ttcgcggcgc gggtgaactg cttggcgaag aacaaagcgg ctcaatggaa
    25861 accatcggtt tctcgctgta tatggagttg ctggaaaacg ccgtcgatgc actgaaagcc
    25921 ggacgcgagc cgtcgctgga agatctcacc agccagcaaa cagaagtcga gctgcggatg
    25981 ccgtcgctat tgccagatga tttcattcct gacgtgaata cgcgtttgtc gttctataaa
    26041 cgtattgcca gcgccaaaac ggaaaacgaa ctggaagaga tcaaagtcga gcttatcgat
    26101 cgcttcggcc tgctgccgga tccggcgcgt atcctgctgg atgttgcccg tctgcgccag
    26161 caagcgcaga aactggggat caggaagctg gaaggtaatg agaaaggcgg ggtgatcgaa
    26221 tttgccgaga agaatcacgt taatccggcc tggttgattg gtttgctgca aaaacagccg
    26281 cagcattacc gccttgatgg tccgacacgc ctgaaattta ttcaggattt gagtgagcgg
    26341 aaaacgcgta tcgaatgggt acgccagttt atgcgtgaac tggaagagaa cgcgatcgct
    26401 taatggtaaa tcgggggcgt ttctgcgccc ccatctgcaa catttacaaa ttctttgcac
    26461 ttccctgcac tatccgacac tgtcaccatc cataattcag gcttatctgt ttattacaat
    26521 aaccttatat ttattatgga tttttggtga tgatcaaaac gcgtttttct cgctggctaa
    26581 cgttttttac gttcgccgct gccgtggcgc tggcgctacc ggcaaaagcc aacacctggc
    26641 cgctgccgcc agcgggcagt cgtctggttg gcgaaaacaa atttcatgtg gtggaaaatg
    26701 acggtggttc tctggaagcc atcgccaaaa aatataacgt cggctttctc gctctgttac
    26761 aggctaaccc cggcgttgat ccttacgtac cgcgcgcggg cagcgtgtta acgatcccgt
    26821 tgcaaaccct acttccagac gcgccgcgcg aaggcattgt gatcaacatt gcggagctgc
    26881 gtctctatta ctacccgccg ggtaaaaatt cggtaaccgt gtatccaata ggtattggtc
    26941 agttaggtgg tgacacgctg acaccgacaa tggtgaccac cgtttcagac aaacgtgcaa
    27001 acccaacctg gacgccaacg gcaaacatcc gcgcccgtta taaagcacag ggaattgagt
    27061 tgcctgcggt agtgccggct ggaccggata acccaatggg ccatcatgcg attcgtctgg
    27121 cggcctatgg cggcgtttat ttgcttcacg gtacgaacgc cgatttcggc attggcatgc
    27181 gggtaagttc tggctgtatt cgtctgcggg atgacgatat caaaacactc tttagccagg
    27241 tcaccccagg caccaaagtg aatatcatca acactccgat aaaagtctct gccgaaccaa
    27301 acggtgcgcg tctggttgaa gtacatcagc cgctgtcaga gaagattgat gacgatccgc
    27361 agctgctgcc aattacgctg aatagcgcaa tgcaatcatt taaagatgca gcacaaactg
    27421 acgctgaagt gatgcaacat gtgatggatg tccgttccgg gatgccggtg gatgtccgcc
    27481 gtcatcaagt gagcccacaa actctgtaaa tcctggataa aaaaaatccc cgcgacacgg
    27541 catgtcgcgg ggaaaaaatc agcagtagca ggcattaatg agggttaatg cttatttata
    27601 aattactgct gttccatgga gggtattcgg accggttaca gaagtaatac ggaaagattt
    27661 tgcgcccatc tcatccgctt tttgcgccag ctgctcttcc agcgatccca gatttgtccc
    27721 cgcgttagca ctgattgtac cgactttttg ttggccttct ggcgttgact gaacttcgac
    27781 agccgcaaag ctggcaaatg acatggagct taaaatcgcc gcagcgatga gggtttttac
    27841 gtttttcata atagtggcct tatgcagatg aatgacgtcg caagcaatta cttaacgatc
    27901 gataactaaa tgatagatgt gatctggatc acatacaaga tat
//
LOCUS       DAZGSP010000067        27142 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZGSP010000067 DAZGSP010000000
VERSION     DAZGSP010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.067"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(10..765)
                     /gene="mlaA"
                     /locus_tag="ACHK74_003122"
     CDS             complement(10..765)
                     /gene="mlaA"
                     /locus_tag="ACHK74_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFP0221892.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            1060..1992
                     /gene="yfdC"
                     /locus_tag="ACHK74_003123"
     CDS             1060..1992
                     /gene="yfdC"
                     /locus_tag="ACHK74_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFP0221893.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2068..2142
                     /locus_tag="ACHK74_003124"
     tRNA            2068..2142
                     /locus_tag="ACHK74_003124"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2101..2103,aa:Arg,seq:cct)
     gene            complement(2583..3830)
                     /locus_tag="ACHK74_003125"
     CDS             complement(2583..3830)
                     /locus_tag="ACHK74_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFP0221894.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(3902..4816)
                     /locus_tag="ACHK74_003126"
     CDS             complement(3902..4816)
                     /locus_tag="ACHK74_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFP0221895.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            5032..6465
                     /gene="cscA"
                     /locus_tag="ACHK74_003127"
     CDS             5032..6465
                     /gene="cscA"
                     /locus_tag="ACHK74_003127"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFP0221896.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(6473..7468)
                     /locus_tag="ACHK74_003128"
     CDS             complement(6473..7468)
                     /locus_tag="ACHK74_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0221897.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            7711..7929
                     /locus_tag="ACHK74_003129"
                     /pseudo
     CDS             7711..7929
                     /locus_tag="ACHK74_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            7947..9275
                     /gene="dsdA"
                     /locus_tag="ACHK74_003130"
     CDS             7947..9275
                     /gene="dsdA"
                     /locus_tag="ACHK74_003130"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFP0221898.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTISLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(9383..10921)
                     /gene="emrY"
                     /locus_tag="ACHK74_003131"
     CDS             complement(9383..10921)
                     /gene="emrY"
                     /locus_tag="ACHK74_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFP0221899.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(10921..12084)
                     /gene="emrK"
                     /locus_tag="ACHK74_003132"
     CDS             complement(10921..12084)
                     /gene="emrK"
                     /locus_tag="ACHK74_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFP0221900.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            12500..13114
                     /gene="evgA"
                     /locus_tag="ACHK74_003133"
     CDS             12500..13114
                     /gene="evgA"
                     /locus_tag="ACHK74_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFP0221901.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            13119..16712
                     /gene="evgS"
                     /locus_tag="ACHK74_003134"
     CDS             13119..16712
                     /gene="evgS"
                     /locus_tag="ACHK74_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFP0221902.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNSDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(16768..17913)
                     /gene="yfdE"
                     /locus_tag="ACHK74_003135"
     CDS             complement(16768..17913)
                     /gene="yfdE"
                     /locus_tag="ACHK74_003135"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFP0221903.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(17987..18931)
                     /gene="yfdV"
                     /locus_tag="ACHK74_003136"
     CDS             complement(17987..18931)
                     /gene="yfdV"
                     /locus_tag="ACHK74_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFP0221904.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(19001..20695)
                     /gene="oxc"
                     /locus_tag="ACHK74_003137"
     CDS             complement(19001..20695)
                     /gene="oxc"
                     /locus_tag="ACHK74_003137"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFP0221905.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(20749..21999)
                     /gene="frc"
                     /locus_tag="ACHK74_003138"
     CDS             complement(20749..21999)
                     /gene="frc"
                     /locus_tag="ACHK74_003138"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFP0221906.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(22512..23144)
                     /gene="yfdX"
                     /locus_tag="ACHK74_003139"
     CDS             complement(22512..23144)
                     /gene="yfdX"
                     /locus_tag="ACHK74_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFP0221907.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            23440..23715
                     /gene="ypdI"
                     /locus_tag="ACHK74_003140"
     CDS             23440..23715
                     /gene="ypdI"
                     /locus_tag="ACHK74_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFP0221908.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(23792..24034)
                     /gene="yfdY"
                     /locus_tag="ACHK74_003141"
     CDS             complement(23792..24034)
                     /gene="yfdY"
                     /locus_tag="ACHK74_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFP0221909.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            24387..25307
                     /gene="lpxP"
                     /locus_tag="ACHK74_003142"
     CDS             24387..25307
                     /gene="lpxP"
                     /locus_tag="ACHK74_003142"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFP0221910.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            25663..25734
                     /gene="ypdK"
                     /locus_tag="ACHK74_003143"
     CDS             25663..25734
                     /gene="ypdK"
                     /locus_tag="ACHK74_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFP0221911.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(25799..27037)
                     /gene="alaC"
                     /locus_tag="ACHK74_003144"
     CDS             complement(25799..27037)
                     /gene="alaC"
                     /locus_tag="ACHK74_003144"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFP0221912.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT         7681 a         6413 c         5967 g         7081 t
ORIGIN      
        1 atttgtttct tattcagaat caatatcttt taaatcatcc tgaatcgctt gtgcgttcgg
       61 attttcctgc ggtttgagtt cgccgccatt agcgatgaaa tcatgacgct ggaagtacgc
      121 ttcgcgcacc ataatataag gatcggacga ctgacgcagc agaccatcgg aatccagcag
      181 ctgagcgcgg gtttcgatcc cttcaagcgt ccatttaccc acagacatcg gccaggtcag
      241 ccaggaaaga accgggtaaa gaccatccgc catatcacca ccgtcatcac gcagcgtgaa
      301 gctaccgtag aacggtaact gaacgtaagg cccataaccc acgccataat gaccaagcgt
      361 actaccgaag cggtgaggtt cagtccgttg cagtttcggg ttcgccatcc ctgcaacatc
      421 aataaagccg cccatcccca aaatggtgtt caggaaaaag cgggtaaagt ggaccatccc
      481 ctgataaggg tcgccctgca agaagtagtt aaccatcacc gcaggttctt caaggttgcc
      541 agtaaagttg ctcaaaccat tacgcgccgg ttgcggaaca taatcacgcc aggcgacagc
      601 gaccggtcga acaatatacg ggtctaatac gttgaagttg aagttgtaca tggtgcggtt
      661 gaacccttct aacgggtcag aacgcccttg ctgatctgta ccggaactcg cacaccccac
      721 cagaagcgta gttcccagag caagcgccga caggcgaagc ttcataaatg tctccctgtt
      781 ttttttatgg cttatgcagt ttgccatcca tgacggaacg ataccgtatc cgcctgttta
      841 ggtgtgggcg attgtaacag cacgtcaact gatgtccaga cgccctgatt tgctgatttg
      901 atcatagcct ggtaatcgcc gccctgtagg ctacttgatt ctatagaaac agaaaaaggc
      961 aaacgcagcc ttttctacat tttcagagta actcccgcca gttgcgagca aaaaagccgc
     1021 tacgctttag ctatacgtgc taattcaaga gaagagacca tggacaacga caaaattgat
     1081 caacacagcg acgaaattga agttgagagc gaagaaaaag agcgcggcaa aaaaatagaa
     1141 atagatgaag accgactccc ctcccgggcg atggcaattc atgagcatat ccgccaggat
     1201 ggtgaaaaag agctggaacg cgacgcaatg gcgctactgt ggtcagccat tgcggcgggt
     1261 ctgtcgatgg gcgcttcgtt actggcaaaa gggatatttc atgtcgaact ggaaggtgtg
     1321 ccgggcagct tcttactgga gaatctcggt tatacctttg gttttattat cgtcattatg
     1381 gcccgccagc aattatttac cgaaaacacc gtgactgcgg tactacccgt catgcaaaaa
     1441 ccgacaatga gcaacgtcgg cttacttatg cgattatggg gcgtcgtgct gctgggtaat
     1501 attctcggga caggtattgc tgcatgggca tttgaatata tgcctatctt caatgaagaa
     1561 actcgcgatg catttgtcaa aatcggcatg gatgtgatga agaacacccc cagcgagatg
     1621 tttgccaacg cgatcatttc cggctggctg atcgccacta tggtttggat gtttcctgct
     1681 gcgggtgcgg caaagattgt ggtgattata ttgatgacct ggcttattgc actgggtgac
     1741 accacccaca tcgttgtcgg ttctgttgaa atcctctatc tggtgtttaa cggcacgctg
     1801 cactggagcg atttcatctg gcccttcgca ctacctactt tagcggggaa catctgcggc
     1861 ggcaccttta tcttcgcgtt aatgagtcat gcacagattc gtaacgacat gagcaacaag
     1921 cgtaaagcag aagcacgcca aaaagcagaa cgtgcggaaa acattaagaa aaatgataaa
     1981 aacccagcat aaatggcgag ggtttaagca atcgagcggc agcgtactta ccccgcagtc
     2041 cattagcggg tatactcatg ccgcattgtc ctcttagtta aatggatata acgagcccct
     2101 cctaagggct aattgcaggt tcgattcctg caggggacac cagataccct tcaaacgaaa
     2161 tctaccttca ccccgtaaaa gataggtttg gcagcacact tgccttatat ctactcattt
     2221 ttactgcaac aggttgaaat ctcagcactg tcagaaagcg ctgatgacta aacagccctg
     2281 ggccgggcga tgtaaccatc acacagaatc ctgatagcga aatatggcgt gactcgatac
     2341 ttcactccgc aatgcattcc ttgatgaatt cgcaggaccg tgatacacgg gacaggtcgc
     2401 tgaatgacga caatgtcctg gaaatcagcg aaccgcgcat ctgaagtaca tttgagcgac
     2461 tgtaccagaa catgaatgag gcgtttggat taggtgatta ttagcagggc taagcacttt
     2521 agtattatta ttttccggtt gagggatagg gagatatcga caacaaccgg aaaagtttac
     2581 gtctatattg ctgaaggtac aggcgtttcc ataactattt gctcgcgttt tttactcagg
     2641 aagaaaatgc caaatagcaa catcaggcag acaatacccg aaattgcgaa gaaaactgtc
     2701 tggtagcctg cgtggtcaaa gagtatccca gtcggcgttg aaagcagcac aatcccaagc
     2761 gaactggcaa tttgaaaacc aatcagaaag atcgtcgacg acaggcgctt atcaaaattt
     2821 gccacgctgt atttgaagac ggatatgaca caaagtggaa cctcaatagc atgtaacagc
     2881 ttcactaatg aaataatcca ggggttaacg aacagcgcgc aggaaaggat acgcaacgcc
     2941 ataatcacaa caccgataag taatgcattt tttggcccta cccgattcac aaagaaagga
     3001 ataatcgcca tgcatagcgc ttcgagtacc acctggaatg agttgagata accatacagg
     3061 cgcgttccta catcgtgtga ttcgaataaa cctgcataaa agacaggaaa aagttgttga
     3121 tcaaaaatgt tatagaaaga ccacgtcccc acaataaata tgacgaaaac ccagaagttt
     3181 cgatccttga aaactgcgat aaaatcctct ttttttaccc ctcccgcatc cgccgctacg
     3241 cactggtgat ccttatcttt aaaacacatg ttgatcatca taaatacagc gccaaatagc
     3301 gagaccaacc agaagttgat atggggactg atactaaaaa atatgccggc aaagaacgcg
     3361 ccaatagcat agccaaaaga tccccaggcg cgcgctgttc catattcgaa atgaaaattt
     3421 cgcgccattt tttcggtgaa gctgtcaagc aaaccgcatc ccgccagata ccccaggcca
     3481 aaaaagagcg cccccagaat tagacctaca gaaaaattgc tttgcagtaa cggttcataa
     3541 acgtaaatca taaacggtcc ggtcaagacc aggatgaaac tcatacacca gatgagcggt
     3601 ttcttcagac cgagtttatc ctgaacgatg ccgtagaaca tcataaatag aatgctggta
     3661 aactggttga ccgaataaag tgtacctaat tccgtccctg tcaaccctag atgtcctttc
     3721 agccaaatag cgtataacga ccaccacagc gaccaggaaa taaaaaagag aaatgagtaa
     3781 ctggatgcaa aacgatagta cgcatttctg aatggaatat tcagtgccat aattacctac
     3841 ctgtcgttaa aaaattcacg tcctatttag agataagagc gacttcgccg tttacttctc
     3901 actattccag ttcttgtcga catggcagcg ctgtcattgc ccctttcgcc gttactgcaa
     3961 gcgctccgca acgttgagcg agatcgataa ttcgtcgcat ttctctctca tctgtagata
     4021 atcccgtaga ggacagacct gtgagtaacc cggcaacgaa cgcatctccc gcccccgtgc
     4081 tatcgacaca attcacagac attccagcaa aatggtgaac ttgtcctcga taacagacca
     4141 ccaccccttc tgcaccttta gtcaccaaca gcatggcgat ctcatactct tttgccaggg
     4201 cgcatatatc ctgatcgttc tgtgtttttc cactgataag tcgccattct tcttccgaga
     4261 gcttgacgac atccgccagt tgtagcgcct gccgcaaaca caagcggagc aaatgctcgt
     4321 cttgccatag atcttcacga atattaggat cgaagctgac aaaacctccg gcatgccgga
     4381 tcgccgtcat cgcagtaaat gcgctggtac gcgaaggctc ggcagacaac gcaattgaac
     4441 agagatgtaa ccattcgcca tgtcgccagc agggcaagtc tgtcgtctct aaaaaaagat
     4501 cggcactggg gcggaccata aacgtaaatg aacgttcccc ttgatcgttc agatcgacaa
     4561 gcaccgtgga tgtccggtgc cattcatctt gcttcagata cgtgatatcg actccctcag
     4621 ttagcagcgt tctttgcatt aacgccccaa aaggatcatc accgacccga cctataaatc
     4681 cacttattcc gcctaatctg gcgattccca ccgcaacgtt agctggcgcg ccgccaggac
     4741 aaggcagtag gcgcccgtct gattctggca agagatctac gaccgcatcc cctaaaaccc
     4801 atactttggc tgacattttt ttcccttaaa ttcatctgag ttatgcatag tgataaacct
     4861 ctttctcgca aaatcgtcat ggatttacta aaacatgcat attcgatcac aaaacgtcat
     4921 agttaacgtt aacatttgtg atattcatcg catttatgaa agtaagggac tatattttta
     4981 taaaagttaa cgttaacaat tcaccaaatt tgcttaacca ggatgattaa aatgacgcaa
     5041 tctcgattgc atgcggcgca aaacgcacta gcaaaacttc atgagcaccg gggtaacact
     5101 ttctatcccc attttcacct cgcgccacct gccggatgga tgaacgatcc aaacggcctg
     5161 atctggttta acgatcgtta tcacgcgttt tatcaacatc acccgatgag cgaacactgg
     5221 ggaccaatgc actggggaca tgccaccagc gacgatatga tccactggca gcatgagcct
     5281 attgcgctag cgccaggaga cgataatgac aaagacgggt gtttttcagg tagtgctgtc
     5341 gatgacaatg gtgtcctctc acttatctac accggacacg tctggctcga tggtgcaggt
     5401 aatgacgatg caattcgcga agtacaatgt ctggctacca gtcgggatgg tattcatttc
     5461 gagaaacagg gtgtgatcct cactccacca gaaggaatca tgcacttccg cgatcctaaa
     5521 gtgtggcgtg aagccgacac atggtggatg gtagtcgggg cgaaagatcc aggcaacacg
     5581 gggcagatcc tgctttatcg cggcagttcg ttgcgtgaat ggaccttcga tcgcgtactg
     5641 gcccacgctg atgcgggtga aagctatatg tgggaatgtc cggacttttt cagccttggc
     5701 gatcagcatt atctgatgtt ttccccgcag ggaatgaatg ccgagggata cagttaccga
     5761 aatcgctttc aaagtggcgt aatacccgga atgtggtcgc caggacgact ttttgcacaa
     5821 tccgggcatt ttactgaact tgataacggg catgactttt atgcaccaca aagcttttta
     5881 gcgaaggatg gtcggcgtat tgttatcggc tggatggata tgtgggaatc gccaatgccc
     5941 tcaaaacgtg aaggatgggc aggctgcatg acgctggcgc gcgagctatc agagagcaat
     6001 ggcaaacttc tacaacgccc ggtacacgaa gctgagtcgt tacgccagca gcatcaatct
     6061 gtctctcccc gcacaatcag caataaatat gttttgcagg aaaacgcgca agcagttgag
     6121 attcagttgc agtgggcgct gaagaacagt gatgccgaac attacggatt acagctcggc
     6181 actggaatgc ggctgtatat tgataaccaa tctgagcgac ttgttttgtg gcggtattac
     6241 ccacacgaga atttagacgg ctaccgtagt attcccctcc cgcagcgtga cacgctcgcc
     6301 ctaaggatat ttatcgatac atcatccgtg gaagtattta ttaacgacgg ggaagcggtg
     6361 atgagtagtc gaatctatcc gcagccagaa gaacgggaac tgtcgcttta tgcctcccac
     6421 ggagtggctg tgctgcaaca tggagcactc tggctactgg gttaacataa tatcaggtgg
     6481 aacaacggat caacagcggg caagggatcc gcgtcactct tcccccttca cgaccttcaa
     6541 taatatgcaa tgcagcttcc cgcccgataa tgtcatgtgg aagctgaatt gtggtcagcg
     6601 gcggtaaaaa cagatgcccg acgccaacca gattatcaaa gcccattacg gcgacatcct
     6661 gcgggattcg tacccccttc gccagaagaa cctgataagc cacaaaggct gcgcgatcgt
     6721 taccacatat cagaacatca aaatctggtt tgcccgattt gaagtggtca ttgagtaaac
     6781 ttgcgagatc ggtgtagtga tcatcacctg ttgccatgtg aaattgtttc acctcagcca
     6841 gatctcgtcc agcatcacgc caggcctgct caaatccctg ccgacgatac cctgttgcca
     6901 acgcactttc cggtagccag aagcataacg gttgacgata gcccgccgcg agcaaatgct
     6961 gtgttgattc atattgtgca gtgtaatcat cagggatata actgggtaac gctgggtcat
     7021 ccgccacaca gttcgccaat acaatatttt caccatacag agactcaggc agcgtgatat
     7081 gtcgcagccc cattgtagta tagataatgc catccggacg gtgggcaagc agctgacgtg
     7141 ccgcgcgggc agcgtcatct tcagaaaaaa tattgattaa aaaactattc cagccgaact
     7201 cgctggcggt ttgctcaatg gcaagcagaa tatcaacaga gaaaggagtg gtagccgtgt
     7261 cctgcgccag cacggcgaga gtcgacggct tacgtccttg agcgcgcatc ttacgggcgg
     7321 aaagatcagg aacataattc agggtctgga ttgcctgcaa tacgcggtca cgcgttgcag
     7381 gacgcacaga ttctgcatta tgcatcaccc gggagactgt catcatcgac actcccgcca
     7441 ggcgtgcgac atcctttaat gaagccatac ccaagccgtt tgccgtaaaa cgggcactgt
     7501 agcagaaaca gacgtcactg gcgagatcca acgatattct caacgcccta tcacccgaca
     7561 cggcaataca ataaaaaata acaataattg ccggataatt gtactcaatt ccgcctctgt
     7621 tctcgccaac gagtaaccat ataggcaagt aagtaaaaca tgccaactgg catcgccaac
     7681 ctggagaaga aaatataaat atggatcatt gtgctgccgc tgtatcccga catcagcccg
     7741 gaaattattg cgattgctat cggttcaggt gcaattggct gcacgatcgt tacggactcg
     7801 cttttctggc tagtgaagca atattgcggc gctacgctca atgaaacatt taaatactat
     7861 acgacagcga catttatcgc ttcagtcgtc gctctggcgg gcacattcct gctgtcattt
     7921 atcatctaag cgcaaagaga cgtactatgg aaaacgctaa aatgaactcg ctcatcgccc
     7981 agtatccgtt ggtaaaggat ctggttgctc ttaaagaaac cacctggttt aatcctggca
     8041 cgatctcatt ggctgaaggt ttaccttatg ttggcctgac cgaacaggat gttcaggacg
     8101 cccatgcgcg cttatcccgt tttgcaccct atctggcaaa agcatttcct gaaactgctg
     8161 ccactggggg gattattgaa tcagaactgg ttgccattcc agctatgcaa aaacggctgg
     8221 aaaaagaata tcagcaaccg atcagcgggc aactgttact gaaaaaagat agccatttgc
     8281 ccatttccgg ctccataaaa gcacgcggcg ggatttatga agtcctggca cacgcagaaa
     8341 aactggctct ggaagcgggg ttgctgacgc ttgatgatga ctacagcaaa ctgctttctc
     8401 cggagtttaa acagttcttt agccaataca gcattgctgt gggctcaacc ggaaatctgg
     8461 ggttatcaat cggcattatg agcgcccgca ttggctttaa ggtgacagtt catatgtctg
     8521 ctgatgcccg ggcatggaaa aaagcgaaac tgcgcagcca tggcgttacg gtcgtggaat
     8581 atgagcaaga ttatggtgtt gccgtcgagg aaggacgtaa agcagcgcag tctgacccga
     8641 actgtttctt tattgatgac gaaaattccc gcacgttgtt ccttgggtat tccgtcgctg
     8701 gccagcgtct taaagcgcaa tttgcccagc aaggtcgtat cgtcgatgct gataaccctc
     8761 tgtttgtcta tctgccgtgt ggtgttggcg gtggtcctgg tggcgtcgca ttcggactta
     8821 aactggcgtt tggcgatcat gttcactgct tttttgccga accaacgcac tccccttgta
     8881 tgttgttagg cgtccataca ggattacacg atcagatttc tgttcaggat attggtatcg
     8941 acaaccttac cgcagcggat ggccttgcag ttggtcgcgc atcaggcttt gtcgggcggg
     9001 caatggagcg tctgctggat ggcttctata cccttagcga tcaaaccatg tatgacatgc
     9061 ttggctggct ggcgcaggaa gaaggtattc gtcttgaacc ttcagcactg gcgggtatgg
     9121 ccggacctca gcgcgtgtgt gcatcagtaa gttaccaaca gatgcacggt ttcagcgcag
     9181 aacaattgcg taatgccact catctggtgt gggcgacggg aggtggaatg gtgccggaag
     9241 aagagatgaa tcaatatctg gcaaaaggcc gttaataacg tttcaacgca gcatcgcaat
     9301 cctttccctg ggtgagcgat gctgccgatg gcgcagactt aagatccccg ctcttaccca
     9361 ctataacccc ctccttttat catcacccaa cgcctttcgc tgtaaacggc ggtttcgcaa
     9421 accaaaccaa aaccgtaagt aagataaaag caatagccgc catacggaaa atctcatttg
     9481 cagaaataga aagtgactgc tgggttattt cattattaat ttcattaaga actcctgaaa
     9541 gcgaaccata gtatttatcc ataatttgtg acgatgaatt aaacacgggg ttaaattgat
     9601 cgatggttgc tgtcaactga ctatggtgta acgattcgcg tcgtccccac agcgtcattg
     9661 tcaacgacgt accaactgat cctgacaagg tacgaaaaaa attactcata ctcgaggcat
     9721 tggcaaattt attatctggc aagcctgaaa acgaaatcgt tgttaagggt aaaaagaaac
     9781 aggcaacggc gaatccctgg aaaaactgtg gcaaaatgat gcctgtaaaa tcaatcgttg
     9841 gcataaatgt cacagaacgc cagtaatagc aaaccgcata catcaaaaaa ctaaatgtca
     9901 ctaacaaccg catgtctatt ttgttgccat aacgtcctat caaaggtgaa attaatagtg
     9961 gcatgatgcc gatgggcgca taagcaagtc cggcccatat cgcattatac cccatcgttt
    10021 cctggagtaa ctgcggcata aggacgatcg ctccagagta aaataaatac gcgcatgtga
    10081 tactcacaat accaatggtg aagttacggg acttaaacaa actgagatca agaatcgggt
    10141 tctctgaggt cgactcccaa atgactaaag agatcagaga aataactgat actactgtta
    10201 atattattat tgtactcgag ttgaaccaat ccagatcgcg ccctttatca agcataattt
    10261 gcaagccacc aacaccgagc actaacaggg tcagtcctgg tagattcatt ttgaccggtg
    10321 aagtttcagt ttctcttcct ttaagtaagg ttaagcataa tgtcaggacg ataatcccca
    10381 tagggacatt gattaaaaat atccaacccc agctaaagtt atcgcaaata taaccgccca
    10441 atatcggccc acatatcgga gcgataatca cggtcattga ccataatgcc agagcaaatg
    10501 ttcttttttc tggtggataa ttccttaata ataaactctg tgacagtgga attaacggcc
    10561 ccgccattaa tccctgaacg actctaaaaa atatcagcac atcaagattg gtcgataaac
    10621 tacacattaa ggaagacagc gaaaaaaaag tgactgaaag taaaaataac cttaattcgc
    10681 ctattctttg tgccagcctg ccggtaacag ggatcgcaat ggcatttgct acgccaaacg
    10741 aggtgataac ccaggtgcct tcgtctgttg atgctcccag aaagccagat attgtcggta
    10801 ttgcgacgtt agaaatagtg gaatccaaca tttgcataaa cgtcgctaat gacaatgcaa
    10861 tagtaacgca ccataacgtc ccaccggtta atggtgccgg agtcgattta gtgattgcca
    10921 ttaaagttgt ccattatgcg aaataatgtt gctaatttct ttttctatcg gactggtatc
    10981 gataactaaa gccttactgg tataagccgg catggacgtc acggttgaag ccagatcagg
    11041 catctcagca atgtcttcgt tcttcgtatc aatagttgca gtcatcgata aaccaatgcg
    11101 taaggggtgt tccatgagtt cttttggatc aagagaaact tcaaccggta cacgctgaac
    11161 gattttgatc cagttccctg tcgcattttg tgcaggtaat aaggagaacg cattgccggt
    11221 tcccatattg atccctgtca cccgaccatg aaacacaaca ttttcaccat aaagatcgct
    11281 gataatattg actgattgac caatccgtac atccgtgagt tgtgtttctt taaagttggc
    11341 attaacccac atttgacgtg ccggtacgac agccattaac gattgtccgg gactcactgt
    11401 ttcgccgacc tgaacactcc gctgggcaat atagccagta accggactcc ttatatccgt
    11461 acgtttaagc gccaaccagg cttctttagt tgcatccgcc gcttccacga cttgtggctg
    11521 acggtttaat ggtgtgttca ttactaaagc tttattcgct ttataagcct ggatagcggc
    11581 attcagtgcc gctttgctac ttattaacgt atctttggta tgctccagcg tttcttttga
    11641 aataaccccc tgcttcgcta acggtactcg acggttataa tcttctaaag attgttgata
    11701 ctgaatacgt gctgaagcga cttcggcact gtattgttta tcctgtaagt acagtttatt
    11761 cgtttgccga acaatatttg ccagattatt tttagcttta ttgagtgcga tagtggcatc
    11821 agttttatcc agtgaaacta aaatgtcacc ttgtcgaacg tagttcgtat ctttatgatt
    11881 aacgacagtg acactacctg agacttgtgc agaaattgga tctgcattcc ccgtgacata
    11941 ggcgtcatct gtactaatca tgtcttctaa ttccattgac caataggcat aggcacctga
    12001 aaacgcaata aataaaacta ccgccaataa agaaaagtat tttcttctgt tagaatgttt
    12061 tttatttgaa ttaatctgtt ccactattat ctctcatttc tcatagatga aatttatgaa
    12121 ttgaatgaaa agagcattca ttcttcatat caaacacagt gcatgtgttg cacattatcc
    12181 caggaacgtt ggaggcctaa tttaaatgag gtgcaaaaaa aacaacagag gactaaaccg
    12241 tggcttttgc aatacaattc ttacgcctgt aggattagtg agaagactta tagtgctaac
    12301 ttgaaactat aaatcatcgg tataatccct gattttattg ttgacatttc atttatgccg
    12361 actatttata tggtatactt gtcgaattat cttaaaggaa gctcagattt tcttattttt
    12421 attgagaaaa tgagatgacg ccttatgtct gtattactac agggagaagg gagatgcttc
    12481 attgcaaagg gaataatcta tgaacgcaat aattattgat gaccatcctc ttgctatcgc
    12541 agcaattcgt aatttattga tcaaaaacga tattgaaatc ttagcggagt tgactgaagg
    12601 gggaagcgcc gttcagcggg tggaaacact taagcctgat atcgtcatca ttgatgtcga
    12661 tatccccgga gttaacggta tccaggtgtt agaaacgctg aggaagcgcc aatatagcgg
    12721 aattattatt attgtctccg ctaaaaatga ccatttttac gggaaacatt gtgctgatgc
    12781 tggcgctaat ggattcgtga gtaaaaaaga aggcatgaac aatatcattg cggctattga
    12841 agctgcaaaa aatggctact gctatttccc cttctctctc aaccggtttg ttggaagttt
    12901 aacgtccgac cagcaaaaac tcgactcctt atcgaaacaa gaaattagtg tcatgcggta
    12961 tattcttgat ggcaaggata ataatgacat tgctgaaaaa atgttcatca gcaacaaaac
    13021 tgtcagcact tataaaagtc gcctgatgga aaaattagaa tgtaaatcac tgatggatct
    13081 ttacacattc gcgcaacgta acaaaatcgg ctaatcacat gaagttttta ccctattttt
    13141 ttcttctctg ttgtggtctt tggtcgacca taagtttcgc agacgaagat tacatcgaat
    13201 atcgtggcat cagtagtaac aaccgtgtca cacttgatcc agtacgtctg agcaacaagg
    13261 aattacgttg gttagcgagc aaaaaaaatc ttgtgattgc agtacacaag tcccaaacgg
    13321 ctaccttgtt gcataccgat tcgcagcaac aggttcgtgg tattaatgct gattatttaa
    13381 atcttttaaa aagagcgtta aatatcaaat taacactccg ggaatacgcc gatcatcaaa
    13441 aagcaatgga cgcgcttgca gaaggtgaag tcgatatagt gttatcacat ttagttactt
    13501 cgccgcctct taatagtgac attgctgcaa ccaaaccatt gataattacc tttccggcgc
    13561 tggtaaccac ccttcacgac tcaatgcgac cgcttacctc accaaaacca gtaaatattg
    13621 ctcgggtagc aaattacccc ccagacgagg taattcatca atcgtttcca aaagcaacaa
    13681 ttatctcttt tacaaattta tatcaggcat tagcatccgt atcagccgga cagaatgatt
    13741 actttattgg tagtaacatc attaccagca gtatgatttc ccgctatttc actcactcct
    13801 taaatgtagt gaaatattat aactcgccgc gtcaatataa ttttttcttg accagaaaag
    13861 aatccgtcat tcttaatgaa gtactcaata gatttgttga tgctttaaca aatgaagttc
    13921 gctatgaagt ttcacaaaat tggcttgata caggaaacct ggcctttctg aacaaaccat
    13981 tagaactcac tgaacatgaa aaacagtgga ttaagcaaca tcccgattta aaggtgctgg
    14041 aaaatcctta ctctcctccc tattctatga cggatgaaaa tggctcggtt cggggcgtta
    14101 tgggggatat tcttaatatt attaccttgc aaacaggttt aaatttttct ccgatcaccg
    14161 tttcacacaa tatccatgcc ggaacacagc ttaaccccgg tggatgggat ataatacctg
    14221 gcgctattta tagtgaagat cgagaaaata atgtttcatt tgcagaagct ttcataacaa
    14281 cgccatacgt ttttgtcatg caaaaagcac ctgacagtga acaaacatta aaaaaaggaa
    14341 tgaaagttgc cattccatat tattatgagc tgcattcgca attaaaagag atgtatccgg
    14401 aggttgaatg gatacaggtc gataatgcca gcgctgcatt tcacaaggtt aaggaaggtg
    14461 aacttgatgc tctggtcgcg acacagttaa attcgcgtta catgatcgat cattactatc
    14521 ctaatgaact ttatcatttt cttattcctg gcgttccgaa tgcatcgctt tcgttcgctt
    14581 ttcctcgcgg agaaccggaa cttaaggata ttattaataa agcactgaat gcaattcccc
    14641 caagcgaagt tctgcgcctg acggaaaaat ggattaaaat gcccaatgtg accattgaca
    14701 catgggacct atatagcgag caattttata ttgttacgac attatccgtt ttattagttg
    14761 gcagtagcct tttatgggga ttctacctgt tacgctcagt tcgtcgtcgt aaagtcattc
    14821 agggtgattt agaaaaccaa atatcattcc gaaaagcact ctcggattcc ttaccgaatc
    14881 caacttatgt tgtaaactgg caaggtaatg tcattagtca taatagtgct tttgaacatt
    14941 atttcactgc ggattactac aaaaatgcaa tgttaccatt agaaaacagt gactcaccct
    15001 ttaaagatgt tttttctaat gcgcatgaag tcacagcaga gacgaaagaa aatcgaacaa
    15061 tatacacaca ggtatttgaa attgataatg gcatcgagaa aagatgcatt aatcactggc
    15121 atacattatg caatcttcct gcaagtgaca atgcagtata tatttgtggt tggcaagata
    15181 ttactgaaac gcgtgatcta attaatgcac tcgaggtaga aaaaaataaa gcgataaagg
    15241 ctaccgtagc aaaaagtcag tttctggcaa cgatgagtca cgaaataaga acaccaataa
    15301 gctctattat gggcttcctg gaacttctgt cgggttctgg tcttagcaag gagcaacggg
    15361 tggaggcgat ttcacttgcc tacgccaccg gacaatcact cctcggctta attggtgaaa
    15421 tccttgatgt cgacaaaatt gaatcgggta actatcaact tcaaccacaa tgggtcgata
    15481 tccctacttt agtccagaac acttgtcact ctttcggtgc gattgctgca agcaaatcga
    15541 tcgcattaag ttgcagcagt acatttcctg atcattacct ggttaagatc gaccctcagg
    15601 cgtttaagca ggtcttatca aatttactga gcaatgctct caaatttacc accgaggggg
    15661 cagtaaaaat tacgacctcc ctggttcaca ttgatgacaa ccacgctgtt atcaaaatga
    15721 cgattatgga ttctggaagt ggattatcgc aggaagaaca acaacaactc tttaaacgct
    15781 acagccaaac aagtgcaggt cgccagcaaa caggatctgg cttaggctta atgatctgca
    15841 aagaattaat taaaaatatg cagggcgatt tgtcattaga aagtcatcca ggcataggaa
    15901 caacatttac gatcacaatc ccggtagaaa ttagccagca agtggcgact gtcgaggcaa
    15961 aagcagaaca ccccatcaca ctacctgaaa agttgagcat attaatcgcg gatgatcatc
    16021 cgaccaacag gctattactc aaacgccagc taaatctatt aggatatgat gttgatgaag
    16081 ccactgatgg tgtgcaagcg ctacacaaag tcagtatgca acattatgat ctgcttatta
    16141 ctgacgttaa tatgccgaat atggatggtt ttgagttgac tcgcaaactc cgtgagcaaa
    16201 attcttcctt acccatctgg gggcttacag ccaacgcaca ggctaacgaa cgtgaaaaag
    16261 ggttaagttg cggcatgaac ttatgtttgt tcaaaccgtt gaccctggat gtactgaaaa
    16321 cacatttaag tcagttacac caggttgcgc atattgcacc tcagtatcgc caccttgata
    16381 tcgaagccct gaaaaataat acggcgaacg atctacaact gatgcaggag attctcatga
    16441 ctttccagca tgaaacgcat aaagatctac ccgctgcgtt tcaagcacta gaagctggcg
    16501 ataacagaac tttccatcag tgtattcatc gcatccacgg tgcggctaac atcctgaatt
    16561 tgcaaaagtt gattaatatt agccatcagt tagaaataac acctgtttca gatgacagta
    16621 agcctgaaat tcttcagttg ctgaactctg taaaagaaca cattgcagag ctggaccagg
    16681 agattgctgt tttctgtcag aaaaatgact aaatagcggc tcccacaatg ttcaaatgtg
    16741 ggagctattt accagcacat cttacattta tgatgagaac tcctggcgaa tttgttcccc
    16801 atgctggtcg agcgttgccg ctcccggcat aacatgcggg tccgcgcagc cgctgatttt
    16861 tatcggatta cccggcatca ttattccccc ggcttcaatc aacatatttc tcgcctgagt
    16921 ttgtggcaat ttaatggcct cagccacact taataacggc gcgacgggta caccaacttc
    16981 atgtattctg gctaaccaaa cttcagctgc ctgcgttttt aacgtccgct caatatattg
    17041 tttaagaata gcctggtttt gtacgcgtaa aatattgctg ctaaatcggg gatcattaac
    17101 cagttccgta agctccagtg cctggcataa cgcagaaaaa agcttgtcat taccacaaca
    17161 aatcgtaatc ggcttatcct gagtattgaa aacatcaaaa ggtgccatgt agggatggcg
    17221 atttcccaga cgttgtggtg acttcccagt tgcgatatat gccatcagac catgctccag
    17281 aaaactcagc gtggcatcaa acatcgctat atcgacatgc gcccctctct ggctcttttc
    17341 gcggccataa agtgcactca ctattccgct gaataaatag acaccgccgc atagatccgc
    17401 aagagaggta ccaacgcgca ctggcggagc atcaggatat cctgtttcca tcattatccc
    17461 gctcattgcc tgaatgatgg tatcgtaggc aggagcatct tttagcggac cggtatgtcc
    17521 gaaacctgac gatgaagcat atatgaggcg cgggttgatt tcttgaagcg tttcccatga
    17581 aaaccccagt ttttccattg tacctgggcg aaaattctca gctaatacat cagcttgttt
    17641 gagcatattt ataaatatac ttttatcgtg atcattcttt aaatcaagaa ccacactctc
    17701 tttgccatga ttaataaaac tgtaatagag tgactgtcca tccacatagg gaccaaatgt
    17761 gcgggtatca tcaccatgac ccggcggctc aactttaatt acccttgcgc ccatattaca
    17821 aagaagttga gttccgaaag gtccattaag gacatgtgtc atatcgataa ctaataagcc
    17881 ttcaaacggc cctttgcttt cattatttgt catttgctta tgccttgaaa aaagccgaag
    17941 gtgaaaagac acttcggcat ttatgtaaaa tcaataacat gattaattat gaaaccagtc
    18001 gactgacata aatccacaag ggagccgtga cgacaaagcc cagtacgctc acagccaatg
    18061 acgctgtacc agtacgggtg tagacattaa accggctggc aataatgatt ccggagaatg
    18121 ccggcggtaa tgcgcctgcc agtaccatca tctgcagatg ttcgctgttc aaatgacatg
    18181 ccataccaac gagaagcagt gccagtggca tcagaatcag cttcaggaag gtgttataag
    18241 caatttcggc actgaactcg aatttatgtg cagccagagt caaccccgca gcgaataccg
    18301 ctacccctga gttagccttc gcaatcagat taaaggttgg gtcccatgct gccggaattt
    18361 ttaccccaac caacaccagg atcgttgcca gaacaggtgc ccatactact ggctcctttg
    18421 ccgcagaaat taatgcgctc agattactat tcttcttacc atccgctcct gaggaaggat
    18481 tcagcaaata cagaccaata ggaatagtaa ttgcgttaac aataatagaa ataattgcta
    18541 ccactaaacc tgttgatacg gaatcaccat aaataggatc gagaactgca aaccccagga
    18601 atccaatggt aggtgaaccc gcaattaatg cacatacagc tgcttctgca tgggtacgtt
    18661 taaaaaattt gtagcaacca aaccaggaga aaaagaaaca tccgacaata accacaagtg
    18721 atacaagggt cagacgagtg tccgcaaaaa tcatttccct gtttgcccgg gtaatagaaa
    18781 caaatagagc cgcaggaaga gcgtagttta ataccagttt attaaatgcg cgggcttgat
    18841 cttctgaaaa agtttctcgt ctgccgctaa aataacccaa cagcatgatt acgataatag
    18901 gcaataaatc gccaataaaa aatgttagca taatagtatt tccgataagg gtcccctcac
    18961 catcgcataa tgagttggca agaggagacg atgtcagtat ttaattacca gcgacttgtt
    19021 ttgggttaag tttggtaata tggccacttt cagttcctgc tgcagggtcg atgaccacat
    19081 taataatggt cggtttgcgc gactgaatac cggtggttaa agcatgacga agttcatctg
    19141 tcgtggtgac gttatagcca acgccacgaa acgcatccat taatttgtca taccttgcat
    19201 ggtgcaacag atccgttggt gatggtgcac cagcgccact gagatcaaca ccgtctcctc
    19261 tgtagatgcc gccattatta aaaataacga tcgtcaccgg caggttatat cgacaaatcg
    19321 tttcaatttc catcccactg aaaccaaaag cactatcacc ttcaatggcg acaaccggag
    19381 aaccagaagt cacgctagca ccgatggcat agcccatacc gatgcccatg acaccccagg
    19441 taccacaatc cagacgacga cgtggtttat acatatcaat aatatttcgt gcattatcca
    19501 gggtatttgc accttcatta actaaataaa tatcctggtt ctcgcgcaat acgtcgcgca
    19561 cagcacttaa tgcattaaag taatttaatg gttgagtatc tgtacttaat ttttcatgca
    19621 ttttttgtgc attttgctgc ttgtggatat ttaaaatatc gcgccatacc agtggagtcg
    19681 taaatgtgtt ttgtttcagt tctgccagca taccttgcat actggatgca atatcaccaa
    19741 cgactggcac agcaatgggg cggttgctgt caatttcctg cggttcaata tccagttgaa
    19801 taaactgtgt atctgccgcc catccttttt taccgtgtgc caataaccaa ttcagtcgtg
    19861 caccaacaag catgacaacg tcagcatttg ccagggcaaa cgaacgcgca gctgccgcag
    19921 aaagtggatg cgtatcttca aggatccctt tcgccataga cattggcagg aatggaatct
    19981 gagtactttc aataaattca cgaagctgtt catcagcttg tgaatacgcc gcgcctttgc
    20041 caaggataat taatggccgt tcagcttttg ctaaaagcga aattgcgcta gtgactgact
    20101 tcgggcatgg caataatgct ggcgacggat tttcaacttt aacaatcgtg gttaacgctt
    20161 cgtctttttc catcgtcgcg gccaggacat ttgctggcaa atcaagataa actccgccag
    20221 ggcgacccga tacagagacc cggatagcgc gtgccaatgc aatgccaaga tcctgcggct
    20281 gattaacgcg aaatgctgct ttggcatacg gttttgccgc attcatttgg tccagctctt
    20341 cataatcacc ttgctgtagg tcgacgatcg cgcggtcgct ggagccgcta atcatgatca
    20401 tcggaaaacc atttaccgtt gcgttggcca atgcggtcaa accattgagg aatcctggcg
    20461 cagaaactgt caggcagatc cccggttttt gggtaagaaa accgcttgcc gcagcggcat
    20521 agcctgccga ctgctcatga cgaaaaccaa tataacgaat gccttccgcc tgggcatggc
    20581 gtgccatatc cgtcacagga atacctacaa caccataaat agtgtcaata ttattctgtt
    20641 ttaatgcttc aacgatgata tgcataccat ctgtcatttg aagttgatct gacatgatta
    20701 ataacctcga aatttttata aattgggagc cagaagcccc cgttaatatc agatggcgtg
    20761 gttttgcttc attgcagcaa tttcatcgtc gctataaccc agctcctgca atacagcagc
    20821 ggtatgttca cctaatagcg gcgcagcttt aatatccggc gtaaaggcag agaatttcat
    20881 tggacaacca acggtcagat attttccacg caacggttgt tccacttcaa caacactgcc
    20941 actttggcgc aaagagggat caagtgaaat ttctttcata cttaaaaccg gtgcacaagg
    21001 aatatcaaac tgagtcaaat aggccaccgc ttcatgttta tcaatagtga cagtgtattt
    21061 ttcgatttca gcaaaaatat cgaaaatatg tggctgtcgt gcatgggctg tactgtatgc
    21121 cggatcggta atccattctg gtttgccgat ggctttacag gtgttttccc agttttgctc
    21181 ctgaatagtg aaataaatat aggcgttagg atcggtttcc cagcctttac atttcaggat
    21241 ccagccaggc tgaccgccac cacctgcatt accaccgcgg ggaactgcat caccaaatgt
    21301 accattcgga tactgcgggt attcttccag ataacccaat ttatcgagac gctgctggtc
    21361 acgtaatttc acgcggcaaa ggttcaatac ggcatcctgc attgacatgg tgactcgttg
    21421 cccacgcccc gttttttcgc gatgcagcaa agcagcaagt aaaccgatca gcaaatgcat
    21481 tccggtgttg ctgtcaccca acgctgcagc gcttaccagc ggcggaccat cccaaaaacc
    21541 cgtagtggat gccgcgccac ccgctgcctg agcaacgttt tcataggctt ttacattcac
    21601 ataaggcgaa cactcatcaa accctttgat cgaaccaaaa atcagacgtg gattgatttc
    21661 ttgaatatgc tcccaggtga agcccatgtg atcaatggcc cctggatgaa agttctcgac
    21721 taagatatca gcttcgcgga tcagcttttc cattacctct ttgccttccg ctgttttggt
    21781 atttaactca atagaacgtt tgttactgtt aagcatggtg aagtaaagcg catcgatatc
    21841 aggaatatct cgcagctggt gacgcgttac gtcaccaacg ccgggacgtt caattttaat
    21901 aacgtcagcg ccaaaccagg ccagcatttg agtacaagat gggccagatt gcacaccggt
    21961 gaaatcgaga actttaattc cttgaagtgg agttgacata gcaataccta ttagttaatt
    22021 aaatatgtgt taatttagcg tttatttaag gcgtaatatc ttctataaat aacccgcaaa
    22081 atatcgtcgt agctgcactc gatattttta aaatcctgta tcaagtgctg aaaatcattt
    22141 catcgccact tccaattcct ttctgcatgg taagtagtga ggacgaaaag aagaatatga
    22201 taaatattga ttcgtggtaa tcagagagag tcgcgacatt tgtgatgtta ttcttacaac
    22261 tgtaagaagc gccggtgatt tacaagtaag ggtatgatta cgctttttaa agcgtaccat
    22321 aaaacataac ataataaatg atttgtgatg acacgggatt attgggattt actttgagga
    22381 agataaaatg cgtgtttata gaaccgcgag cgccaaataa ttttatgacc acaacctgtc
    22441 gtattgtggt cataaaaaat tgtcggctgg agggagttaa atcctttacc tcaatatact
    22501 gcctgtcagc attagttcac aaacagtgct tcgctgtcga cgatgattcc atcttccgca
    22561 cctttaagcg ccaggtttgc ctcgtagtat tgctttttat caagcagttt ctgcgcatca
    22621 gcaagtgcat tacgagtctg tttaagcggc atcagatatt ggttttccat tacgccaaca
    22681 ccggccaggc gaagttcttc cattgcgccc tttttatccc ctttcgccat tttttcattg
    22741 gcaattttta tcgcggcttc tttttcggga gttgctacat aactctcaga aattccgaca
    22801 gatgcattaa tgacaatata ttggtcatca ttgagattgg tctttttacc tggcttagcg
    22861 aactttgccc attcggtact gtcatctgac agcaaagcgg aagcttcatt ggtcagttct
    22921 tttgcctttt ctggatcgcc atgaaacaga gccaggcgcg ctacctgcac gtcacgcatc
    22981 gcatataaac cttgttcaga gatacgctct gctgctgccg ttttctgggt ttgctgaact
    23041 tgctgagttt gctgttgtgc cgctaccggt gcattatcag cagcccatac agttgaagat
    23101 gccagaattg ctgtgaccat cgtagccata attaaacgtt tcatacttac ctcattcttt
    23161 aacagtttta gaaaacgccg ccatcatatt gggtgagcat gaaatgagaa atcagagagc
    23221 aacggtcatt ttgaaagggt tatcttacag ttgtagttat tatcgcaaga agtattttag
    23281 tcttacaatt gtaggaatct gcttcctgtc gcagagaact attcttatgt accacaaaga
    23341 gatgtcattt attatatcga tgtaaatatt gattaattat atgtaaatac agccataaca
    23401 tcgtaggttc tcagtgaacc attttcagga taactttcta tgaaagtaaa cttaatactt
    23461 ttcagcttat ttttattggt ctctattatg gcatgcaatg tttttgcatt ttccatttct
    23521 ggtggtgtaa gtgagaggag ctataaagag actgaaaaaa catcagcgat gacaaccaca
    23581 cactctacaa aacttcagcc atcacaggcg attttgttta agatgagaga agatgcgcca
    23641 ccattaaacc tcacagaaga aatcacgcct acttatccga caaaggcgaa ttatcttatt
    23701 catcctgtgc gatagcgtct gtcacatggc aggtgaacaa tcatgtctga tgctgatgtg
    23761 aacacctgcc caaatgagcc atcctgaccc actatcgcct gccatacccc accataaagc
    23821 gaatcatcaa gccacaagcc ggggcaagaa acataaatat ccccgtcact agttcattac
    23881 cgccagttaa ccacatggtg aagtaataaa tgagtgcagc gaggatcacc atgccgaaga
    23941 aagaagagac cgccgatacc acgttcagta cctgaccgat gtagccgctt acgcagacaa
    24001 tacacagggc gagaaacatc cataaataaa tcatagctca acctcctgcc gcgctaaaaa
    24061 acctgtaact tcagtcactt taatagtaag acaatcctta acctctggca atttttgcta
    24121 agcatcaatg aaaacagcat gttaaatgca agactgttgt gtacggaaaa atatttactt
    24181 tgcacgatta ataatcagta gctgaaagca gtcagcgagt gataaagtac agataccgca
    24241 ttcatgctcc tcgtggttat gtcctgacta gtctttacac tctttacagg aaccattgtc
    24301 gtacatgatg gcccaaccaa ttgagtgtac cgctgtcgac atggaatctt ctgcgataca
    24361 acaattcgta tctacagaag gtaactatgt ttccacaatg caaattttcc cgcgagtttc
    24421 tacatcctcg ctactggctc acatggtttg ggcttggtgt actctggctt tgggtacagc
    24481 ttccttatcc tgttctctgc tttctcggca cgcgtattgg cgcaatggcg cgaccattcc
    24541 tgaaacgtcg tgaatctatc gcccgtaaaa acctggaact ttgtttcccg cagcattctg
    24601 cggaagaacg cgagaagatg attgccgaaa actttcgttc actcggcatg gcgctggtag
    24661 aaaccggcat ggcatggttc tggcccgaca gtcgcgtacg taaatggttt gatgttgaag
    24721 ggttggataa ccttaaacgc gcacaaatgc aaaatcgcgg cgtaatggtt gtcggcgtcc
    24781 attttatgtc gctggaactg ggcggccgcg tgatgggact gtgccaacca atgatggcta
    24841 cctatcgtcc acataataat cagctgatgg aatgggtgca gacccgtggg cgcatgcgct
    24901 ctaacaaagc gatgatcggc agaaataatc tgcgcggcat tgtcggtgca ctgaagaaag
    24961 gtgaagcggt atggtttgct cccgatcagg attatggtcg taaaggcagc tccttcgcgc
    25021 cgttctttgc ggtggaaaat gtcgccacaa ccaatggcac ctatgttctc tcccgtctct
    25081 ctggcgcagc catgttgacc gtaacgatgg taagaaaagc ggattacagc ggatatcgtt
    25141 tgttcatcac cccagagatg gaaggctacc cgacagatga aaatcaagcc gctgcctata
    25201 tgaacaagat tatcgagaaa gagatcatgc gcgcaccgga gcagtacctc tggatccacc
    25261 gtcgctttaa aacgcgcccg gtgggagaat cgtcgttgta catttaacaa actgctctta
    25321 ctctttccct cctcaaatcg gccataataa cctcatggtt tttatggctt ttttcttaag
    25381 gttttttcct tcgctgaata tctaattatt accgtcagaa attaaactgt cgccgatatg
    25441 agacacttgt aagttactaa aagtaaataa gaaaatgtct cttgtctcgt ttcagtttta
    25501 ggcgtacatt agcgccgtct cgagcagttt agctcagaat tctggggtga tgcccacggt
    25561 aaaatacagg gcttttaagg tcgctttgcc cggtgtcaac tcactgtatc aggtgtaatg
    25621 aagtcattca ggcgtaacag taattacgcg gagagatgta aagtgaaata tttctttatg
    25681 ggcatttctt ttatggtcat cgtttgggcc ggtacttttg ccctgatgat ctaaaagcaa
    25741 actgtgcaat gaaaaacagg agccatcagg ctcctgtttg catttctgac gacggtgttt
    25801 attccgcgtt ttcgtgaata tgtttgctgc tggcgggtag taaaccgtcg gcacggaaca
    25861 tcgctttaat cccacgaatc gcctgacgaa tacggtcgcg gttttcgatc agtgcaaagc
    25921 gaacatgcgt atcaccatag tcgccaaagc caatccccgg cgagacacaa accttcgctt
    25981 cgttaaggag cttcttggca aattccagcg atcccatggc cgcatatggt tccgggattt
    26041 tcgcccagac atacatcgaa gccttcggca tttcaaccat ccagcccgct tcatgcagcc
    26101 ctttaaccaa tacatcacgg cggcgtttgt actgttcggc aatgtcgcgc acgcactgtt
    26161 gatcgccctc cagcgccgca atcgccgcca cctgcaacgg cgtaaaggtg ccgtaatcgt
    26221 gatagctttt aatacgagcc agggcgctga ccagcgtttt gttgccaacc ataaagccga
    26281 tacgccagcc cgccatgttg tagcttttcg acagcgtaaa gaactcgact gccacatcgc
    26341 gtgcacccgg tacctgcatg attgacggcg ctttccagcc atcgtagacg atatcggcat
    26401 aggccaggtc gtggaccacc agcacatcgt agcgtttcgc cagcgccaca accttttcga
    26461 agaactccag ctccacgcat tgcgcggttg gattcgacgg gaagccgagg atcatcatct
    26521 tcggtttcgg ataactttca cgaatggcgc gctccagttc gttgaagaaa tcgacacctt
    26581 ccaccagcgg cactgagcgt acctgcgccc cggcaatcac cgcgccataa atatggatcg
    26641 ggtaacttgg attcggcacc agcaccgtgt caccatgatc cagcgtcgcc agcatcagat
    26701 gcgccaggcc ctctttcgaa ccaatagtga cgatggcttc tgattccggg tcgatttcaa
    26761 cgtcgtagcg atcctgatac cagcgggaaa tggcgcgacg taaccgcgga atgccgcgtg
    26821 aagtggagta accatgcgtg tccgggcgct gggccacagt acataatttt tcgacgatat
    26881 gcggcggagt cgcaccgtcc gggttaccca tgctgaaatc gataatatct tcgccgcgcc
    26941 gacgcgcagc cattttcagt tcagcggtga tgttaaaaac gtagggcggg agacgatcaa
    27001 tgcgcgtaaa gcgacgttca gggcgagtgt cagccataat ttcctcagat taacgttagc
    27061 gcccggaccg tccgagcgac gctgccacaa tggtggctgt tttgaaaata gcctgattaa
    27121 tttctgtctg tccagcgttt ta
//
LOCUS       DAZGSP010000068        27038 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZGSP010000068 DAZGSP010000000
VERSION     DAZGSP010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.068"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..645
                     /locus_tag="ACHK74_003145"
     CDS             <1..645
                     /locus_tag="ACHK74_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221913.1"
                     /translation="YSNEYANSGVAALLQYCSSKMDLPSNNDTYHEMYHALNMNLSSK
                     NFEDGHISTGTIFFDTESNKWYLCVSAACDLVPTQGNDPHHVRLSPHRLIKVLELFNA
                     SQSKALPFAEHSKYIYVMHKNQRKYLSIFEGDKTLPVVDYMVVLNHGTTVDGEEKNII
                     SAVFLSNMDGNVQNVPVRLKLKSQLRTGYAERYQAIASQYSSRICVDYVSMMLP"
     gene            complement(717..1133)
                     /locus_tag="ACHK74_003146"
     CDS             complement(717..1133)
                     /locus_tag="ACHK74_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0221914.1"
                     /translation="METLFKVFEKFSSRPLFFIFFGLSLCEFFQKQSVLMNPSADNIA
                     KLFAAMILVVFFTWGFEWLIFKFNVNLEPHDQGDIGPTIGTATLAVYLVYAFHFLSEN
                     PEALNLKLLTNSGFIYSTTLLLFSLECMKLRRLKQK"
     gene            complement(1172..2401)
                     /locus_tag="ACHK74_003147"
     CDS             complement(1172..2401)
                     /locus_tag="ACHK74_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFP0221915.1"
                     /translation="MAKIAKKLTDTEIKSTKPADKEINLFDGDGLILRIAPLAKGGKK
                     NWYFRYAVPVSKKRTKMSLGTYPHLTLARARALRDEYLSFLANGVDPQIHNNDKAKAL
                     KSATEHTLQAVARKWLDEKVKTSGISQDHAADIWRSLERNVFPGLGNVPINEIRPKLL
                     KQHLDPIEQRGVLETLRRIISRLNEIFRWAATEELIEFNPADNLGQRFSKPKKQNMPA
                     LPPSELPRFMESLTNASIRLETRMLIEWQLLTWVRPGEAVRARWSDIDTTNSIWNIPA
                     DFMKMKKLHKVPLSKEALRILELMKSISGHREWVFPSIKAPLNHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTAAEESGKFRAEVLEAALAHSKKDEIIAAYNRAEYLIERQSLM
                     QWWSDYVQAQRSNALVA"
     gene            complement(2567..2929)
                     /gene="ssrA"
                     /locus_tag="ACHK74_003148"
     tmRNA           complement(2567..2929)
                     /gene="ssrA"
                     /locus_tag="ACHK74_003148"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(3144..3626)
                     /gene="smpB"
                     /locus_tag="ACHK74_003149"
     CDS             complement(3144..3626)
                     /gene="smpB"
                     /locus_tag="ACHK74_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFP0221916.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            3797..4234
                     /gene="ratA"
                     /locus_tag="ACHK74_003150"
     CDS             3797..4234
                     /gene="ratA"
                     /locus_tag="ACHK74_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFP0221917.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            4224..4514
                     /gene="yfjF"
                     /locus_tag="ACHK74_003151"
     CDS             4224..4514
                     /gene="yfjF"
                     /locus_tag="ACHK74_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFP0221918.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(4576..4917)
                     /gene="bamE"
                     /locus_tag="ACHK74_003152"
     CDS             complement(4576..4917)
                     /gene="bamE"
                     /locus_tag="ACHK74_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFP0221919.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(5066..6727)
                     /gene="recN"
                     /locus_tag="ACHK74_003153"
     CDS             complement(5066..6727)
                     /gene="recN"
                     /locus_tag="ACHK74_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFP0221920.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(6813..7691)
                     /gene="nadK"
                     /locus_tag="ACHK74_003154"
     CDS             complement(6813..7691)
                     /gene="nadK"
                     /locus_tag="ACHK74_003154"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFP0221921.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            7814..8407
                     /gene="grpE"
                     /locus_tag="ACHK74_003155"
     CDS             7814..8407
                     /gene="grpE"
                     /locus_tag="ACHK74_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFP0221922.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(8462..9748)
                     /gene="yfjD"
                     /locus_tag="ACHK74_003156"
     CDS             complement(8462..9748)
                     /gene="yfjD"
                     /locus_tag="ACHK74_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFP0221923.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(9769..10560)
                     /gene="ypjD"
                     /locus_tag="ACHK74_003157"
     CDS             complement(9769..10560)
                     /gene="ypjD"
                     /locus_tag="ACHK74_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFP0221924.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            10727..12088
                     /gene="ffh"
                     /locus_tag="ACHK74_003158"
     CDS             10727..12088
                     /gene="ffh"
                     /locus_tag="ACHK74_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFP0221925.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            12225..12473
                     /gene="rpsP"
                     /locus_tag="ACHK74_003159"
     CDS             12225..12473
                     /gene="rpsP"
                     /locus_tag="ACHK74_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFP0221926.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            12492..13040
                     /gene="rimM"
                     /locus_tag="ACHK74_003160"
     CDS             12492..13040
                     /gene="rimM"
                     /locus_tag="ACHK74_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFP0221927.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            13071..13838
                     /gene="trmD"
                     /locus_tag="ACHK74_003161"
     CDS             13071..13838
                     /gene="trmD"
                     /locus_tag="ACHK74_003161"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFP0221928.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            13880..14227
                     /gene="rplS"
                     /locus_tag="ACHK74_003162"
     CDS             13880..14227
                     /gene="rplS"
                     /locus_tag="ACHK74_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFP0221929.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(14304..14786)
                     /gene="yfiB"
                     /locus_tag="ACHK74_003163"
     CDS             complement(14304..14786)
                     /gene="yfiB"
                     /locus_tag="ACHK74_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP0221930.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(14802..16028)
                     /gene="dgcN"
                     /locus_tag="ACHK74_003164"
     CDS             complement(14802..16028)
                     /gene="dgcN"
                     /locus_tag="ACHK74_003164"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFP0221931.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            complement(16018..16536)
                     /gene="yfiR"
                     /locus_tag="ACHK74_003165"
     CDS             complement(16018..16536)
                     /gene="yfiR"
                     /locus_tag="ACHK74_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFP0221932.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            complement(16686..17051)
                     /gene="yfiL"
                     /locus_tag="ACHK74_003166"
     CDS             complement(16686..17051)
                     /gene="yfiL"
                     /locus_tag="ACHK74_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFP0221933.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            17261..18331
                     /gene="aroF"
                     /locus_tag="ACHK74_003167"
     CDS             17261..18331
                     /gene="aroF"
                     /locus_tag="ACHK74_003167"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFP0221934.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRRTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            18342..19463
                     /gene="tyrA"
                     /locus_tag="ACHK74_003168"
     CDS             18342..19463
                     /gene="tyrA"
                     /locus_tag="ACHK74_003168"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFP0221935.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRMMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(19506..20666)
                     /gene="pheA"
                     /locus_tag="ACHK74_003169"
     CDS             complement(19506..20666)
                     /gene="pheA"
                     /locus_tag="ACHK74_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFP0221936.1"
                     /translation="MTSENPLLALREKISTLDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     misc_feature    complement(20688..20813)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(20765..20812)
                     /gene="pheL"
                     /locus_tag="ACHK74_003170"
     CDS             complement(20765..20812)
                     /gene="pheL"
                     /locus_tag="ACHK74_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFP0221937.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            complement(20916..21257)
                     /gene="raiA"
                     /locus_tag="ACHK74_003171"
     CDS             complement(20916..21257)
                     /gene="raiA"
                     /locus_tag="ACHK74_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFP0221938.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            complement(21528..22265)
                     /gene="bamD"
                     /locus_tag="ACHK74_003172"
     CDS             complement(21528..22265)
                     /gene="bamD"
                     /locus_tag="ACHK74_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFP0221939.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            22400..23380
                     /gene="rluD"
                     /locus_tag="ACHK74_003173"
     CDS             22400..23380
                     /gene="rluD"
                     /locus_tag="ACHK74_003173"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFP0221940.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            23377..24108
                     /gene="yfiH"
                     /locus_tag="ACHK74_003174"
     CDS             23377..24108
                     /gene="yfiH"
                     /locus_tag="ACHK74_003174"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFP0221941.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            24238..26811
                     /gene="clpB"
                     /locus_tag="ACHK74_003175"
     CDS             24238..26811
                     /gene="clpB"
                     /locus_tag="ACHK74_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFP0221942.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         6742 a         6264 c         7002 g         7030 t
ORIGIN      
        1 tattcaaatg aatacgctaa cagcggagtt gctgcattgc tccaatattg ctcttcaaaa
       61 atggatctgc catcaaataa tgatacttat cacgaaatgt atcatgcttt aaatatgaat
      121 ttgtcttcaa agaattttga agatggtcat atttctactg gcactatttt ctttgataca
      181 gagtcgaaca aatggtattt atgtgtatct gcggcatgtg atttggttcc tactcagggt
      241 aacgaccctc accatgtaag attaagtccg cacaggctca ttaaagttct ggagcttttt
      301 aacgccagtc agagtaaagc attgccattt gctgaacatt cgaaatatat atatgtaatg
      361 cataaaaatc aaagaaaata tctctctatt ttcgaagggg ataaaacgct tcctgttgtt
      421 gattatatgg tggtgttgaa tcatggaaca acagttgatg gcgaagaaaa aaatattatt
      481 tctgccgtgt ttttaagtaa tatggatggc aacgtgcaaa atgttcctgt ccgactcaaa
      541 cttaaatctc aactgagaac tggttatgca gaaagatatc aggctatagc gtctcagtat
      601 agctcaagga tttgtgtgga ttatgtatca atgatgctac cataattatt atattttagg
      661 cgtggtgatt ttttttcgcc atgcctattt tatatttatc atcacaatga tgttgtttat
      721 ttttgtttaa gtcttctaag cttcatgcat tctaatgaga ataataatag agttgtgctg
      781 tatataaagc cagagttagt taataacttt aaatttaatg cttcaggatt ttcactgaga
      841 aagtgaaagg cataaactaa gtatacagct aaagtagccg ttccaattgt tggtccaata
      901 tcgccttgat catgaggttc aaggtttaca ttgaacttga agattagcca ttcaaatccc
      961 caagtaaaaa aaacaactaa tatcatggct gcgaataatt tcgcgatgtt atctgctgat
     1021 ggattcatca gaacagattg tttctgaaaa aattcacaaa gtgagagtcc gaaaaaaata
     1081 aaaaaaagtg gtctggaact aaatttttca aaaactttga atagggtttc cattttcttt
     1141 accttcagga ttatattagc tattctgata cttaggctac cagagcattt gatctttgag
     1201 cttgaacgta atcactccac cattgcatca aactctgtcg ctctatcaga tattctgcac
     1261 gattgtatgc tgcgataatt tcatcttttt tcgagtgggc aagcgctgcc tcaagaactt
     1321 cagctctgaa tttaccagac tcctctgccg ctgttcgtgc aatagaacgc ataccgtgag
     1381 ctacaagctc gcctccgaac cccattcgga tgatagctgc gttggctgtt tgttcatgca
     1441 tatgattaag aggcgctttt atgctgggga aaacccattc tctatgccca cttattgatt
     1501 tcattaattc aaggatgcgc aaagcttctt tactcaaagg aactttgtga agctttttca
     1561 ttttcatgaa atcagcagga atgttccaaa tgctgttggt tgtatcaata tcagaccacc
     1621 ttgcgcgaac ggcttcaccc ggacgaaccc atgtcaacaa ttgccattca attagcatac
     1681 gtgtttccaa ccggattgac gcattcgtca aagattccat aaaccttggc aattcgcttg
     1741 ggggaagggc aggcatattt tgcttttttg gtttactgaa tctttgacca aggttgtcag
     1801 ccgggttgaa ctcaataagt tcttcagtag ctgcccaccg gaagatttca ttcagacgtg
     1861 aaatgatacg gcgtagagtt tccaatacgc ctcgttgctc aataggatca aggtgttgtt
     1921 ttaagagctt aggtcggatc tcattgatag ggacattacc cagaccggga aagacatttc
     1981 tctctaagct gcgccagatg tctgctgcat ggtcttgtga gatacctgat gtctttacct
     2041 tctcatctaa ccatttccgc gctacggctt ggagagtgtg ctcagtagca ctctttaatg
     2101 ccttcgcctt atcgttgtta tggatttggg gatcaacacc atttgccaga aaggagagat
     2161 attcatcacg taaggctctg gctcttgcaa gggtaaggtg aggatatgtc ccaaggctca
     2221 ttttggttct tttcttgctc actggtactg catacctgaa ataccaattt ttcttgcctc
     2281 ctttcgccaa aggagcgatt cgtagaatca gaccatcacc gtcaaacaag ttgatttctt
     2341 tatcggctgg cttggtgctt ttgatttcag tgtcagtgag cttcttagcg atttttgcca
     2401 ttttgggacc ctcggttttt ggacccttct tagtgggtcc cattcagggt gccataactc
     2461 gtagttctca gcaattctca ctggacgaca atagacgtaa aaaagcccgc agagcttgtg
     2521 ctgtgtgggc ttagtagact tcattgaact tcaaacaact aaaaagtggt ggagctggcg
     2581 ggagttgaac ccgcgtccga aattcctaca tcctcggtac tacatgctta gtcagtcttt
     2641 acattcgctt gccagctgcg gacggacacg ccactaacaa actagcctga ttaagtttta
     2701 acgcttcaac cccaggcagg gcttccacgc gatctctttt gggtttgacc tctcttgatc
     2761 cccgtcctaa gagcggaggc tagggagaga gggctctaag caggttatta agctgctaaa
     2821 gcgtagtttt cgtcgtttgc gactattttt tgcggctttt tacgaggcca accgcccctc
     2881 ggcatgcacc ttgggtttcg caaatcccgt cgaatccaga atcagcccca atgtgtaaag
     2941 gtaagtatac cagatttatg agcgccatga ccagcctcaa tggcgttatc gttaaagatt
     3001 tagcacccat gtagcctgat ttttattcga ttaagcaatg ggatggcaac atttgtgtcg
     3061 gatgtgatag ccaataagat gttcattcgc gccgccggag agggaggcgc ggtgaggaac
     3121 tggtcaataa ttggagtgca ggtttaacgg tgggcgtttt tcatgatacg tgctttatcc
     3181 acctgccatt cgcgttcttt gatatctgaa cgtttatcgt gctgtttttt acctttggcg
     3241 acgccgattt tcactttgca ccaggcattt ttccagtaca gggagagcgc cactacggta
     3301 tagccttctc gattgacgcg accgtacaat gagtccagtt cgcgctggtt gagaagtaac
     3361 ttgcgggtac gggtaggatc gcacaccaca tgcgtggagg ccacggccat tggcgtgatg
     3421 ttagcgccaa acagaaatgc ctctccgtca cgcagaagga cgtagctgtc gctgatattg
     3481 gcttttcctg cgcgcaggga tttaacctcc cagccttgca gggcaagtcc cgcttcgaac
     3541 tcttcttcga taaagtattc gtgacgggcg cgcttgttaa gcgcgatggt cgctgaacca
     3601 ggtttatgtg cttttttctt cgtcataagc gtcgtgaatc atcggtaatc tgaaatcgaa
     3661 aacaccccat atcaatcctg cgggggacaa ggctctatct tagcatgaac ccgatgttac
     3721 ccagcgccgg gatagcgttt tttttacagc aggataaatg atattatttg ttggattttt
     3781 gttgatggaa attgttatgc ctcagattag ccggaccgca ctggtaccct acagcgcgga
     3841 gcaaatgtat cagttagtga atgacgttca gtcttatcct cagtttttgc cgggttgtac
     3901 cggaagtcgg attctggagt ccactcctgg gcagatgact gcggcggtag atgtctctaa
     3961 ggctgggatc agcaaaacgt ttactacccg caaccagttg accagtaacc aaagtattct
     4021 tatgaatctg gtggatgggc cgttcaagaa attgattggt ggatggaagt ttacgccgct
     4081 gagccaggag gcgtgtcgta tcgagtttca tctcgacttt gagtttacca ataagttgat
     4141 tgaactcgcc tttggtcgcg tgtttaaaga gctggcgtct aatatggtcc aggcttttac
     4201 ggttcgtgcg aaagaggttt acagtgccag gtaaaattgc cgttgaggtg gcttatgcgc
     4261 tacctgagaa gcagtacctg cagcgcgtga cgctgcagga gggcgcgacg gttgaagaag
     4321 ctattcgcgc cagtggcttg ctggaattgc gtaccgatat cgatttaact aaaaacaaag
     4381 tcggcattta cagccgtccg gcaaaactaa gcgatagtgt gcatgatggc gatcgggtgg
     4441 agatttatcg ccctctcatt gccgatccga aagagcttcg caggcaacga gcagaaaaat
     4501 cagcgaataa ataacagaca gaaaaaaggt gctcattgag cacctttttt aacgtctttg
     4561 agagcaactt tattattagt taccactcag cgcaggtttg ttatcaatat tggtcaacac
     4621 accgctactg ttaaaggtca gcgtcagcgt ttgctgcgtt acaccttcat gacctggttg
     4681 ctggcggaag acatagaacc aggtattcgt accaaatgga tcggacatca gcggtgtacc
     4741 caatgcgtac gcaacttgtt gttgcgtcat gccaacacgt attttggata cgtcgttagc
     4801 ggtcagatag ttcccctggt tgatgtcagg acggtaaacc actcgctcca gagtggaaca
     4861 gcctgcggtc aacatcaata gtactgctgc tgcagcagtc agcgttttac agcgcatagt
     4921 gatttgattc cttttcgggc ccgagcagta cgtggctcat ctgtaatatg ccgatgataa
     4981 tagacctttc atcactttaa aaccttttgc tttccggtct tacggcgttt tgctgtttac
     5041 tctgaccgtg aagcaggaaa aaagtttacg ctgcaagcag ttctttcgca ttcgccagtg
     5101 tattacgtgt gacttcactg ccaccaagca ggcgcgccag ctcttgtaac cgcgcttttt
     5161 tattcaggga ttgcatatgc gtttctgtca tcgcaccatc ggtttctttg ctgacaaaat
     5221 agtgttgatg accacatccc gcgacttgtg gcaggtgggt aacacacatc acctgagttg
     5281 attcgccgag ttgacgcagc agtttgccga caactgccgc tgttggaccg ctaatcccta
     5341 catccacttc atcaaaaatc agtgccgggg tttccatttt acgcgccgtg atgacctgga
     5401 ttgccagtgc gatgcgggac aattcaccac cggatgcgac tttggcaata ggctgcattg
     5461 gctgacctgg gttggtggtt acccgaaact caatacgatc ggcaccgtca gcgcccaggt
     5521 gatgctcgtc aaatttaaca tcgatcgtaa actgcccatg cggcattgag agcgcatgca
     5581 tactgtcggt gatcagctgt gcaagttctt ctgcatattg ctggcgttgt tggtgtaatg
     5641 cgcgcgcgat ttccagtgcc tgctgatgat gtttcgttac cgccagcgca agcgtttctt
     5701 gtgagtcggc ctgatcgtcc agttgctgct gttcttccag tagcgactgg taatactgtg
     5761 gcaatgcctc agggctgacg tgatgtttac gtgccagcga aatctgtttt gagatgcgct
     5821 gttcaagttc aaatagtcgg ttgggatcga gatccagacg atcgcagtag tggcgcagtt
     5881 catcgctggc ttcagcaatc tggatggtag cttcttccag catatcaagt acgccggaca
     5941 gtttgctgtc catgccaatc aattcgctca ccagttgttt agccgtgtaa agctgacttt
     6001 gcaggtttgc gtcttcaccg tcggccatta atgccaatgc attctggctg gtggtcagca
     6061 attgaccgct gttcgccaga cgtttgtact cttcgtcgat ttgttcaaac tctccgggct
     6121 gcggattaaa ttcgttaagt tcttttaatt ggtattgcag cagttccgca cgggcggcgc
     6181 gttcctgact taactgttga tgatgcgcga ggtcacggca gctttgatgc cacaactgat
     6241 aacgtgcggt catttcctgc agtagagagg tttcattggc atagccatca agcaggaatt
     6301 tttggtgctc aggtttggtg agtaattgat gagcgtgctg accatggatc tgaatcagca
     6361 actgacccag ttcgcgcagt tgtgacagag gaacagctgt accgttgatg aaaccacggg
     6421 agcgaccatc gctgctgatc actcgacgaa gcaaacattc atgcccgtct tcaagctgat
     6481 tttcttccag ccagcgcaga gccgctggcg tatctttcag agaaaaacgg gcgcacaggt
     6541 cagcgcgagc agcgccggta cgcaccatgt cggcttcagc gcgaccaccg agacaaagac
     6601 cgagggcatc tattgcaata gatttacccg cgccggtctc gccagttatt acggtcatgc
     6661 cgctatgaaa atcaatctca agctcacgaa cgatagcaaa gttgctgatg gtcagttgtg
     6721 ccaacatagt cgttttcctg tatgaaaaac cattactgtt attgtgtaca gtataaactg
     6781 gttttatata cagtaaagag gctggcgtaa aattagaata atttttttga ccagccgagc
     6841 ttggtgctta atgtgttgaa ataactgtaa tctttcggat gaatcagatt cagatggtaa
     6901 tcacagcgac gaatcaggac atcttcacct tcctgaatcg gcagtgctat ctggctgtcg
     6961 caactgattt ccaggtcgtt acggcgatgc gaaaaacgca gacggatcgt gctgctgctg
     7021 tttatgacca gtggtcgtgc tgacaacgta tgcgggaaca tgggcaccag ggtaatcgca
     7081 tccagagagg gggtcagaat aggaccgcct gcagagaggg aataggcggt ggagcctgtt
     7141 ggcgtcgaaa taattagtcc atcagatcgc tgagaaaacg caaagatctc gtcgatatac
     7201 acttcgaact caatcatatg cgccactttg cctggatgaa gcaccacttc atttatcgcg
     7261 gtgctgatgc gtttctggca atcttgctga cagacttgcg cttccagcaa aaaacgtttc
     7321 tcgctgatgt agtggccttc cagcacatcg gctaactgtt gctgggcgtt atcggggtca
     7381 aggtcagtca ggaaacccag gttgccacgg ttgattccaa taactttaat atcgtaacgg
     7441 gcgagtgtgc gtgccgcgcc cagcatatta ccgtcgccac caacgactac cgcgagatca
     7501 gctagttgcc caatctccgc gagcgtgcca gttttcacat tcttcagttg cagttcgtga
     7561 gcgatttgtt gctcaacgat gacctcgtaa ccttttgtgc acagccagcg gtagagcatt
     7621 tcatgtgttg tcagtgcagt ggggtgccgt gggtgtccca caatgccaat acacttgaaa
     7681 tgattattca tttttccgag gtccttgttg cgaagattga tgacaatgtg agtgcttccc
     7741 ttgaaaccct gaaactgatc cccataataa gcgaagttag cgagatgaat gcgaaaaaaa
     7801 cgcggagaaa ttcatgagta gtaaagaaca gaaaacgcct gaggggcaag ccccggaaga
     7861 aattatcatg gatcagcacg aagagattga ggcagttgag ccagaagctt ctgctgagca
     7921 ggtggatccg cgcgatgaaa aaattgcgaa tctcgaagct cagctggctg aagcccagac
     7981 ccgtgaacgt gacggcattt tgcgtgtaaa agccgaaatg gaaaacctgc gtcgacgtac
     8041 tgaactggat attgaaaaag cccacaaatt cgcgctggag aaattcatca acgaattgct
     8101 gccggtgatt gatagcctgg atcgtgcgct ggaagtggct gataaagcta acccggatat
     8161 gtctgcgatg gttgaaggca ttgagctgac gctgaagtcg atgctggatg ttgtgcgtaa
     8221 gtttggtgtt gaagtgatcg ccgaaactaa cgtgccgctg gacccgaatg tgcatcaggc
     8281 tatcgcaatg gtggaatctg atgacgttgc gccaggtaac gtactgggca ttatgcagaa
     8341 gggttatacg ctgaatggtc gtacgattcg tgcggcgatg gtaactgtag cgaaagcaaa
     8401 agcgtaattt ctgttttcgt aataattcac ggccctgcat gcgaatgcag ggccgttttc
     8461 gttactccgc cacactctcg cgcagcggtt tcacaggaaa aacttttacc tgcttaatca
     8521 tattgtcctg tacgtcgaga atatctatat cgtactcgcc aatacgcacg cgggtgcctg
     8581 cgacagggat ctcttccagt gcctcaagaa tgacgccatt aaccgtgcgg gcatcatctt
     8641 ccggtagatg ccagttaaag gctttgttga tttcccgcac gttggcggtg ccatcgataa
     8701 tcaccgaacc gtcgttttgc ggcgtgacct cttcggcaag tgttggcgac atcgacgtag
     8761 tgaaatcgcc gacaatctct tccagaatat cttcaaccgt caccagcccc tgaatgtcgc
     8821 catactcgtt gacgaccagg ccgactttct ttttgttgcg ctgaaacttt accaactgcg
     8881 tgctgagcgg cgtaccttcc ggcacaaaat agatctcgtc cgcggcgcgc agcatggttt
     8941 ctttggtgaa ctcttttttc tccgacatca accgccaggc ttcacgtact cgcagcatac
     9001 tgatggcgtc gtccagcgaa tcacggtaga gcacgatgcg cccgtgaggt gagtgggaga
     9061 gttggcgcag aatcgatttc cagtcatcgt tgatatcaat accgataatt tcactgcgcg
     9121 gcaccatgat gtcatcaacg gtcatttttt ccagatcgag caccgacagc agcatatcct
     9181 gattgcgacg ggaaatttgt gagcgtgatt cgtgcacgat agtgcgcaac tcttctttgc
     9241 tcaaagagcc gctaaccacg atatcggttt tgatacccat catgcgcatc agcatacggg
     9301 tgatagcatt cagcaaccag accagcggca tcatcaaaat ttgcagcgga gccagcagaa
     9361 aactactcgg gtaagcgact ttttccgggt acagcgcggc aatggttttc ggcaatacct
     9421 cagcaaatac cagtacgaca aaagtcagca ctccagtcgc aattgccacg cccgcatcgc
     9481 cgtacaaacg catcccaaca atagtgccga gcgcggaggc aagaatattg accaggttat
     9541 tgccgattaa caccaggctt atcaggcggt ctggcttacg cagcaatttt tcgacgcgtt
     9601 tggccgagcg attaccctgt ttcgccatat gtcgcagacg atagcggttg agggtcatca
     9661 ttccggtttc ggacccggaa aaataggctg aaatgaccac catgatgatc agaataatga
     9721 tcaacgtagt agtagaaatg tgttccaggg gaaactcctt ttctgggttt agctgattaa
     9781 ctgctggaca attcggctgc cgaagtaggc cagtgtcaga attaccgcgc ccgcaacgtt
     9841 aaaccagacg acgcggcgtc cacgccatcc ttcatggtaa tgtccccaca gcagcacaat
     9901 atagacaaac cacgccacga tagagagcac agccttgtcg atattttcca tgctaaatag
     9961 gttgtgcatg tagaacaggc cagtgcaaag cgtgagcgtt agcagcacca cgccaatctg
    10021 cgtgatgtgg aacattttac gctcgatact catcaatggc ggcatttcct ggttaaatgc
    10081 cagcttcttg ttcttcagtt ggtaatcaat ccacgccagt tgcagcgcgt acagggcggc
    10141 gataattagc gtggcatagg aaaagagcga taagccaatg tgcaccagca tcccaggcgt
    10201 tgcttccaga tgggtgatgt attcattggg catgaaggtc gccagcgcca ggttgataag
    10261 cgcaaaggca tagacaatgg gtagcagcaa ccagccacga ttgcgagaag ccacaatggt
    10321 cattaccgta cagatcatca aactgaccaa tgaaccaacg ttcagcaggc tgaggttttg
    10381 tccgctatca ccgtcgggca ggatgcgggc ttccagagcg attgcgtggc agaccagcgc
    10441 aatgaccgca gaaataatag ccatgcgccg ccagccgccg tttttttgca gcagaccggg
    10501 aacaatcagc gcaagactga cggagtaggc gacaagcgcg agcagagcaa aaacgggcat
    10561 agtgatgtcg acagtttgag caataagaaa gagaagcagt atagcgtcag gtgaacgctg
    10621 ctccaaccgt tgcataacaa caaagacgcc ttcatgttat actgcggcaa aatactgatg
    10681 atgtgtcgcg attgcggcca accgtttcca ccccaggcga gagacaatgt ttgataattt
    10741 aaccgatcgt ttgtcgcgca cgctgcgcaa tatcagtggc cgtggacgcc tcactgaaga
    10801 caacgtaaaa gatacgctgc gcgaagtgcg catggcgctg ctggaggcgg acgtcgctct
    10861 gccggtagtg cgtgagttta tcaatcgcgt aaaagagaaa gcggttggtc atgaagttaa
    10921 taagagcctg acgccggggc aggagttcgt caaaatcgtc cgtaacgaac tggttgcggc
    10981 gatgggcgaa gagaaccaga ccctgaacct ggcggcgcaa ccgcctgcgg tcgtgctgat
    11041 ggcgggcctg caaggtgccg gtaaaacaac cagcgttggt aaactcggta agttcctgcg
    11101 cgagaagcac aagaagaaag tgctggtggt ttctgccgac gtttatcgcc cggcggcaat
    11161 caaacagctt gagacgctgg cagagcaggt gggcgttgat ttcttccctt ctgatgttgg
    11221 tcagaagccg gtagatatcg ttaacgcggc gctgaaagaa gccaaactga aattctacga
    11281 cgtgctgctg gtggataccg ctggtcgtct gcacgttgac gaagcgatga tggacgagat
    11341 caaacaagtc catgcgtcga ttaacccggt tgaaaccctg tttgtggttg acgccatgac
    11401 cggtcaggat gcggccaata cggcaaaagc attcaatgaa gcgttaccgc ttaccggcgt
    11461 agtgttgacc aaagtggacg gcgatgcccg cggcggtgcg gcgctctcta ttcgtcacat
    11521 cactggcaaa ccgatcaagt ttctcggtgt tggcgagaaa actgaggcgc tggagccgtt
    11581 ccatccggac cgcatcgcgt cgcgtattct cggcatgggc gacgtactgt cgctgatcga
    11641 agatatcgaa agcaaagttg accgcgcgca ggcagagaaa ttagccagca agctgaaaaa
    11701 aggcgacggc ttcgatctca acgattttct tgagcaactg cgccagatga aaaatatggg
    11761 cggcatggct agcctgatgg gcaagctgcc gggcatgggg cagatcccgg ataatgttaa
    11821 gtcgcagatg gacgataaag tgctggtgcg tatggaagcc atcatcaact cgatgacgat
    11881 gaaagagcgc gctaagccag aaatcatcaa aggttcgcgt aagcgtcgta tcgctgccgg
    11941 ttgcggtatg caggtgcagg acgtgaaccg ccttctgaag cagttcgacg acatgcagcg
    12001 catgatgaag aaaatgaaga aaggcggaat ggcgaagatg atgagaagca tgaaggggat
    12061 gatgccgccg ggatttcctg gtcgctaaat gaccttatgc gacagagtta ttaactgctg
    12121 attgcatttt ctccagaaat cagtaaaatt ttcgggcttt taatatgaca ccggactccg
    12181 ttcctcgatg gggtccggtt gttttattca cacaagagga tgttatggta actattcgtt
    12241 tagcacgtca cggcgctaaa aagcgtccgt tctaccaggt tgttgtcgct gacagccgta
    12301 atgcacgcaa cggtcgcttc atcgagcgcg ttggtttctt caacccaatc gctagcgaaa
    12361 aagaagaagg cactcgcctg gatctggatc gcatcgctca ctgggttggc cagggcgcaa
    12421 ctatttctga tcgcgttgct gcgctgatca aagaagtaaa caaagcagct taatctgtca
    12481 cggtggtcat gatgagcaaa caactcaccg cgcaagcacc tgttgatccc atcgttttgg
    12541 gaaaaatggg ttcgtcttac ggtattcgtg ggtggctcag agtgttttct tccaccgaag
    12601 acgccgaaag catttttgac tatcagccct ggtttatcca gaaggcgggt cagtggcagc
    12661 aagtccagct ggaaagctgg aagcaccaca atcaggacat gatcatcaag ctgaaaggcg
    12721 ttgacgatcg tgatgcggcg aacctgctga cgaattgtga aattgtcgtg gattcatcgc
    12781 agctgcctca gcttgaagag ggtgactact actggaaaga cctgatgggc tgccaggtag
    12841 taaccactga aggctacgat ctcggtaaag tcgtcgatat gatggaaacc ggatctaatg
    12901 acgttctcgt cattaaggca aacctgaaag atgcgtttgg tatcaaggaa cgtctcgtac
    12961 cgttcctcga tgggcaggtt atcaagaaag tcgatctcac tactcgttca atcgaagtag
    13021 attgggatcc tggtttttaa accaccggat aaacggtaaa agacggcgct atgtggattg
    13081 gcataattag cctgtttcct gaaatgttcc gcgcaattac cgattacggg gtaactggcc
    13141 gggcagttaa aaatggcctg ctgagcatcc agagttggag tcctcgcgac ttcacgcatg
    13201 accggcaccg taccgtggac gatcgtcctt acggcggcgg accggggatg ttaatgatgg
    13261 tgcaaccctt gcgggacgcc attcatgcag caaaagccgc ggcgggggaa ggcgcaaagg
    13321 tgatttatct gtcaccacag ggacgcaagc ttgatcaagc gggcgtcagc gaactggcaa
    13381 cgaatcaaaa attgattctg gtgtgcggtc gctacgaagg tatagatgag cgcgtgatcc
    13441 aaaccgaaat tgacgaagaa tggtcaatcg gcgattacgt tctcagtggt ggtgagttac
    13501 cagcaatgac gctgattgac tccgtttccc ggtttattcc gggagtactg ggacatgaag
    13561 cctcggcaac ggaagattcc tttgctgaag gattgctgga ttgcccgcac tatacgcggc
    13621 ctgaggtgtt agaagggatg gaagttccgc cagtgttact gtcgggcaac catgccgaga
    13681 tacgtcgctg gcgtttgaaa cagtcgctgg gccgtacctg gcttagaaga cctgaacttc
    13741 tggaaaacct ggctctgact gaagagcaag caaggttgct ggcggagttc aaaacggaac
    13801 acgcacaaca gcaacataaa catgatggga tggcgtaagc ccccgagata tcagtttacc
    13861 caggataaga gattaaatta tgagcaacat tattaagcaa cttgaacaag agcagatgaa
    13921 gcaggacgta ccttccttcc gtccgggtga taccgtggaa gtgaaagtat gggttgttga
    13981 aggttccaaa aaacgtctgc aggcattcga gggcgtggtt atcgctattc gtaaccgcgg
    14041 tctgcactct gcattcactg ttcgtaaaat ttccaacggc gaaggcgttg agcgtgtctt
    14101 ccagactcac tctccggtag ttgacagcat ttctgtcaaa cgtcgtggtg ctgttcgtaa
    14161 agctaaactg tactacctgc gtgagcgtac tggtaaggct gctcgtatca aagagcgtct
    14221 taactaagat ttcgcttaag cgacatcctg ttaagaaggg ctggccaatt ggctggccct
    14281 ttttttgtct gtttgctcgt gttttaaggg gttgtaatca ccactgcgac gcggcggttc
    14341 tcggcgcggc cctgggcggt cttgttactg gctatgggat atttttttcc taaaccctgt
    14401 gtggtgagat tgctgcgtgg aatttgtcca cccatagccc atgcatcggc cacgacattc
    14461 gcccgtttca atgataagcc ttcgttgtaa ctgtcttcac catagttatc ggtgtgtcca
    14521 tccatacggg catgtgttag ccctgtcgag gccaatttgg ctgccatggt ttggatctgt
    14581 tgctggcttt ccgggagcaa tttgtagtca ttttttgcga acagaatggc atctgataag
    14641 cccagcgacc agtcgccggc ggattcagta aatccataag attgcatagc ggcgacttgc
    14701 tcaggagtaa actttccctg aggggactgg cagccagtca gtattagtga ggtgaaaatc
    14761 aggggtgcta ctaggtgctt tatcatatcg atatatcctt gttatctcac cagcttttcg
    14821 gcacgctggt gtttggcctg atacatattg tgatcggcaa gctcttgtaa tttttccgca
    14881 gaggcgtgct caatagtcat cgcgtaacca atgcttaatg tcatggtggt ctgatggcca
    14941 ttatgaagat caaacgggag attaaagatt tgtgtcagtg ctgagcatat ctgctgcact
    15001 tcagattccg actgtacatc atagagcacc atagcgaatt catcgccgcc caggcggtat
    15061 gctttatgtc gcagcccgcc aaattcagct aaccgttttg cgatttcaat caagactcta
    15121 tcgcccgtcg catgacccca ggtatcattg atatatttga aattatcgcc atcaagaaat
    15181 agtaacgccg acgtttttcg ggcatcggaa ttgttcatca acgtattgat gccgctacga
    15241 aacgctgcgc ggttagccag cccggttaat gggtcatgta gtgcggtacg cagaagctgc
    15301 gcatttttgg cctgtaaacg aagctgccac tcttccattt catccagcag actgttgaag
    15361 tcgagagcga agcggtgaaa ctcagcaata cgttcttccg aaactcgtcg ggaaaaattg
    15421 cggttggaac gcacatcatg tacgacatcg gtgatatttt tcagtgcttc caccaggcca
    15481 ttgtgcaaat ggcgggtgag ggtaattgcg atgcctgatg ccagcagaat acaaccggtc
    15541 agtacggcga gcgaaaacca gataaaatgg ctgattgaac tgtcgcgagc ggttaagcgt
    15601 acttcgccaa tggtttcacc attgtgacga atcggctgaa tgatgggggc agggaagagc
    15661 cagtggctta tgaaattgct gaaagtatcg cctggatcct tacgggtgta atgccaggat
    15721 gccagaatat tttgctgctt atcacgtact tctgcagttg aaaattgccc ttgttggccc
    15781 agcgctgcca gtgtttcagt tgctgcaggg ccatcggcaa aaacgacagc tgcttccaga
    15841 ctgtaagtca ttgttgctgc tgtcagtgcc aggttttttt gcgcgtactg tttcagggtc
    15901 agcaccgagg tcacggaaag cagcaaccag atcagcatca tagtgataaa tatgctggtc
    15961 atactgatgt tgcgtaatgc tcttttaaac gtggggcgct tattaagaga attatcgtta
    16021 tccatcattt ttcttccgtg cgagcattaa gacatccggg ttgaccttta caccgctacg
    16081 cgataaggca tccaggttta cggcaaatct gacgtcatta ttgtggatga tcagacaaaa
    16141 ggcgctacca ataatgcact cggtattttg ttcggcaatt aataataacg cctttgacgg
    16201 atattgttcg gttaattcca tctggaatgt cggtgactcg ttgccaaaat aaaaaccatt
    16261 gcagcctgaa atcatcgcct cttgttgagt gtgaatgatg acaggcagat aaggcaaaga
    16321 tgtcgcggca ttctcttgta gggcggtact aaaacgtgaa gatgaaaaga tacataactt
    16381 tggtggccct gatagcgcag gccagcgggt gtaactgaca ataccggaaa ccatcatacg
    16441 cacgtttttc gcaacgtcag aaggctcctg agcgagtatt ggtgctcccg tcagcaggag
    16501 aatcagaagt aagaacagtc ggtgagaaaa acgcattcag ggatccccac catcttcgct
    16561 cgaaatgcat cgatattttt tcagaatgct cattaaaata tcattttttc atgcgaccgt
    16621 catcaatgtg agaaataaaa cacaatgagt aaaatcttat gtttaattta tttgtggggt
    16681 tttaattaat ttaatcgtat tgtgctggca ttttcatcag cccctttttg ccatacttca
    16741 tgcagttggc tggcattttc aacattgttg cagctggcag gaaagctttt accggaaagt
    16801 cctcgggcat aagtaaaatc ggtctggcaa gttttctttt gtccttcggc atatccttta
    16861 aggtatagac cgcgatccgc ctgtgaatcg ctaaatgcat cgtcatcttt tatggcgctg
    16921 cccgatatcg catcttccat accaacatca taccagtcgg tgctggtcaa ggttggcgcg
    16981 tgagtataag gatcaatctg acatccgcta accagtaaag ccaacaaggg ggcgataaac
    17041 tttttcatca tttctttctc ctttttcaaa gcatagcgga ttgttttcaa agggagtgta
    17101 aatttatcta tacagaagta agggttgaaa tcgcgactaa attgcctgtg taaataaaaa
    17161 tgtacgaaat atggattgaa aactttactt tatgtgttat cgttacgtca tcctcgctga
    17221 ggatcaacta tcgcaaacga gcataaacag gatcgccatc atgcaaaaag acgcgctgaa
    17281 taacgtacat attaccgacg aacaggtttt aatgactccg gaacaactga aggccgcttt
    17341 tccattgagc ctgcaacaag aagcccagat tgctgactcg cgtagaacca tttcagatat
    17401 tatcgccggg cgcgatcctc gtctgctggt agtatgtggt ccttgttcca ttcatgatcc
    17461 ggaaactgct ctggaatatg ctcgtcgatt taaagccctt gccgcagagg tcagcgatag
    17521 cctctatctg gtaatgcgcg tctattttga aaaaccccgt accactgtcg gctggaaagg
    17581 gttaattaac gatccccata tggatggctc ttttgatgta gaagccgggc tgcagatcgc
    17641 gcgtaaattg ctgcttgagc tggtgaatat gggactgcca ctggcgacgg aagcgttaga
    17701 tccgaatagc ccgcaatacc tgggcgatct gtttagctgg tcagcaattg gtgctcgtac
    17761 aacggaatcg caaactcacc gtgaaatggc ctccgggctt tccatgccgg ttggttttaa
    17821 aaacggcacc gacggcagtc tggcaacagc aattaacgct atgcgcgccg ccgcccagcc
    17881 gcaccgtttt gttggcatta accaggcagg gcaggttgcg ttgctacaaa ctcaggggaa
    17941 tccggacggc catgtgatcc tgcgcggtgg taaagcgccg aactatagcc ctgcggatgt
    18001 tgcgcaatgt gaaaaagaga tggaacaggc gggactgcgc ccgtctctga tggtagattg
    18061 cagccacggt aattccaata aagattatcg ccgtcagcct gcggtggcag aatccgtggt
    18121 tgctcaaatc aaagatggca atcgctcaat tattggtctg atgatcgaaa gtaatatcca
    18181 cgagggcaat cagtcttccg agcaaccgcg cagtgaaatg aaatacggtg tatccgtaac
    18241 cgatgcctgc attagctggg aaatgaccga tgccttgctg cgtgaaattc atcaggatct
    18301 gaacgggcag ctgacggctc gcgtggctta agaggtttat tatggttgct gaattgaccg
    18361 cattacgcga tcaaattgat gaagtcgata aagcgctgct gaatttatta gcgaagcgtc
    18421 tggaactggt tgctgaagtg ggcgaggtga aaagccgctt tggactgcct atttatgttc
    18481 cggagcgcga ggcatctatg ttggcctcgc gtcgtgcaga ggcggaagct ctgggtgtac
    18541 cgccagatct gattgaggat gttttgcgtc ggatgatgcg tgaatcttac tccagtgaaa
    18601 acgacaaagg atttaaaaca ctttgtccgt cactgcgtcc ggtggttatc gtcggcggtg
    18661 gcggtcagat gggacgcctg ttcgagaaga tgctgacact atcgggttat caggtgcgga
    18721 ttctggagca acatgactgg gatcgagcgg ctgatattgt tgccgatgcc ggaatggtga
    18781 ttgttagtgt gccaatccac gttactgagc aagttatcgg caaattaccg cctttaccga
    18841 aagattgtat tctggttgat ctggcatcag tgaaaaatgg accattacag gccatgctgg
    18901 cggcgcacga tggcccggta ctggggttac acccgatgtt cggcccggac agcggtagcc
    18961 tggcaaagca agttgtggtc tggtgtgatg gacgtaagcc ggaagcatac caatggtttc
    19021 tggagcaaat tcaggtctgg ggcgctcggc tgcatcgtat tagcgctgtc gagcacgatc
    19081 agaatatggc gtttattcag gctctgcgcc actttgctac ttttgcttac gggctgcacc
    19141 tggcagaaga aaatgttcag cttgagcaac ttctggcgct ctcttcgccg atttaccgcc
    19201 ttgagctggc gatggtcggg cgactgtttg ctcaggatcc gcagctttat gccgacatca
    19261 ttatgtcgtc agagcgtaat ctggcgttaa tcaaacgtta ctataagcgt ttcggcgagg
    19321 cgattgagtt gctggagcag ggcgataagc aggcgtttat tgacagtttc cgcaaggtgg
    19381 agcactggtt cggcgattac gcacagcgtt ttcagagtga aagccgcgtg ttattgcgtc
    19441 aggcgaatga caatcgccag taataatcca gtgccggatg attcgcatca tccggcacct
    19501 tttcatcagg ttggatcaac aggcactacg ttctcacttg ggtaacagcc caataccttc
    19561 attgaacggg tgatttcccc taactctttc aatgcttttt gcatttccgc tgattcaaga
    19621 ttggcctgaa tatccagata aaacatctct tcccatggat taccgtgaat cgggcgtgat
    19681 tccagacggg tcataatcag attgtggttg cgcagtacca gcaacgcttc aaccagcgca
    19741 ccggcttgtt gcccggtcgc cattaacaac gtggttttcg ccggaacctg atcagacacg
    19801 ttaatggctt tacgcgccaa caccacaaat cgggtgaagt tttgtcgctg atttgcttca
    19861 atacgctcca gtacctgcaa accgtacaaa gtgccgccag cttcgcttcc caacgcagca
    19921 acatgcggtg attttgcctg tgcaaccttt tccattgccg cagacgtact ttcggtatat
    19981 tcaatcttcc agtgtggata acgattaagg aatttgctgc attgctggaa tggctgcgga
    20041 tggctgtaga ccgtattgat ggtggataaa tcagtagtgc cggagaccaa caaacaatgg
    20101 tcgatagtta acgtcatctc gccaacaatc gacaagctgg tatgttgcag cagatcgtaa
    20161 acgtcgttta tggcaccgga gctggtattt tcaatcggta cgacggcata gtcggcctgg
    20221 ccggtttcca cctgattaaa aatatcggca aatttggcgc agccactttc aatgaattgc
    20281 tcaaagtgac gggcagcata ctggcgcgcc gcaagatggg aataagaacc tttggggccg
    20341 agaaaagcga tgcgtgctga gtgcggatta attttattga gatgttgttg gagcaaagcc
    20401 tgctgagtta atacggaatc ttcaatgatg agctggaaca ggcgagtaat gtaatgggcg
    20461 tccagatggt gcgctttacc gagcgtaatt aatctttcca gtaaatcgcg ttcgcgatca
    20521 atatcacgta ccgggcgatg cgagagcagt ttggcttttc ctacctcgac ggccaattcg
    20581 cgccgctctg ccagtaatgc taataatttt tcatccagcg tgctgatttt ctctcgcagc
    20641 gccagtaacg ggttttccga tgtcatagtg ttgccttttt gttatcaata aaaaaggccc
    20701 cccgatttgg gaggccttat tgttcgtctt cgcattctgt ttcacacgac gaaacgcctc
    20761 ccattcaggg gaaggtaaaa aagaatgcga agaaaaacgg tgtgtgtttc atgtttgttt
    20821 ccttaagtga ctttagtaca gtacccgtac tgttttcacg ctgtcaacaa aaaacgcgcc
    20881 cgaaggcgcg ttggcgatac actcaatata aaggactact cttcttcaac ttcttcgacg
    20941 aagttggcgt ctttcaccga tgttgcggca cgacgtgctt cgcctttgtg ctgcagttta
    21001 ttgagctgcc gttccagctt gttgatcaat tcgttaattg cagtgtacat atcttcatgt
    21061 ttaccactgg caaccagaac gccgttaggt gtattgattg tggcgtcagc aacaaaccct
    21121 tgtggctctt tggacagaat gatatgtgga ttaatcagat gtgtttgcca tttttccagt
    21181 ttggcgagac ggtctgcgac atgttggcgg atggccggag taatttccat ttgtttgctg
    21241 gtaatgttca ttgtcataaa ttttacctct tgtcttcccg tcttggtgaa ttcagcatac
    21301 cgctcctgat gtcaaaatgt gtgatgggaa tctcataatt ttgtcacttt ttgtcaacga
    21361 atcttttttg tgagaaatcg caggaagagg tgttttttta cttgaggaat atctcaaagc
    21421 gagccatatt tgatgagatc gatagtgact aaatcgcttc agtttcacaa ctaacagaat
    21481 aaaaaaacgg cagctcaagg gctgccgttt tgtatttcag gtttctgtta tgtattgctg
    21541 ctgtttgcgg cgatgatttt cgctactttt tcagcttgcg cattcatctg catctgacgg
    21601 tatgcatttt ccatcagcgg cagcgcatca cgcgtagcct gggtatccgg gtagtcgcgc
    21661 aacattcctt ctacgcggtt aacgacggca acccatgcgc cacgttctgt atagtactcg
    21721 gccacggagt attcatattt cgccagacga tctttcagga ataccagacg tttggtggca
    21781 tcggtggtgt actgactgtt tggatagccg cgcaccagtt tggaaaagtc actaaacgca
    21841 gctcgtgcat gttgaggatc gcgatcgcta cggtcaacgc caaagaaccc ttgcagcgca
    21901 ctgtcatcca gcgccatatt ggtcaggcca cgcatgtaca tgacataatc gatattcgga
    21961 tgggtcgggt taaggcgaat aaaacgatcg atggcagcct gcgctaacgg caaatcggcg
    22021 tttttatagt aggcgtagat gagatccagc tgcacctgct gcgaatacgg accaaacgga
    22081 tagcgattat ctaacgcttc cagttgcgtt attgcctgtc tccagttacc gtcctgcagc
    22141 ttttgttgtg cagtcgcgta aatttcattt ggcggattat caggtacttc ttcctttgac
    22201 cccgagcaac ccgccaaaaa caggcttagt gtggcggctg ccaccagata tttcatgcgc
    22261 gtcatgacgt tttgactttc ctcaaaatgt aatacgggag attctctgtt cctgctcccg
    22321 gttaagacca gctacaatag cacactatat taaacggcaa agccgtaaaa ccccaacgat
    22381 aaacgaagaa gcagtatata tggcacaacg agtacagctc actgcaacgg tgtccgaaaa
    22441 ccaactcggt caacgcttag atcaggcttt ggccgaaatg ttcccggatt attcacgttc
    22501 gcgaataaaa gaatggatcc tcgaccagcg cgtgctggta aacggcaaag tttgtgataa
    22561 gccgaaagaa aaagtattgg gtggcgagca ggttgccatc aatgctgaga ttgaagaaga
    22621 agcgcgtttt gaaccgcagg atatcccgct ggatatcgtc tatgaagatg aagatatcat
    22681 cgtcattaat aaaccgcgcg acctggtggt acatcctggc gcgggtaacc cggatggcac
    22741 ggtactgaat gcgttgcttc attactatcc gcccattgcc gatgtaccac gtgcgggcat
    22801 cgtccatcgt ctggataaag acaccactgg cctgatggtt gtggcaaaaa ccgttccggc
    22861 tcagacgcgt ttagtcgaat ctttgcaacg gcgtgaaatt actcgtgagt atgaagcggt
    22921 ggcgattggt catatgaccg ctggcggtac agtagatgaa cccatcagcc gccacccgac
    22981 caaacgtact cacatggcgg tgcatccgat gggcaaaccg gcggtgactc actatcgcat
    23041 catggaacac ttccgtgtgc acacgcgtct gcggttgcgt ctggaaactg gacgtacgca
    23101 ccagatccgc gtgcatatgg cccatatcac tcatccgctg gtgggcgatc cggtttatgg
    23161 tggccgtccg cgtccgccaa aaggtgcttc ggaagcattt atctccatgc tgcgtaagtt
    23221 tgaccgccag gcgcttcatg caaccatgct gcgtctttat cacccgatct ccggcatcga
    23281 aatggaatgg catgcgccta ttccacaaga tatggtggag ctgattgagg tgatgcgcgc
    23341 cgatttcgaa gagcataagg atgaagtgga ctggttatga gtaagctgat tgtcccgcag
    23401 tggccgctac caaaaggtgt tgcggcctgt agctcttctc gtatcggcgg cgtgagcttg
    23461 cccccgtatg actcgctcaa cctcggtgcc cattgtggcg ataacccgga tcacgttgag
    23521 gagaatcgca agcgactttt tgctgcgggc aatttgcctt ctaaaccggt ctggcttgag
    23581 caggtacacg gcaaagatgt gcttaagctt actggcgaac cttatgcctc aaaacgagca
    23641 gatgcctctt atagcaatac gcccggcacg gtttgcgcag tgatgactgc cgactgcctc
    23701 cctgtgctgt tttgcaatcg agcgggaacg gaagtcgccg ccgctcatgc tggctggcgt
    23761 ggactgtgcg caggcgtgct ggaagagacg gtttcctgtt ttgctgataa tccggaaaat
    23821 attctcgcct ggttagggcc ggcaattggt ccacgcgcgt tcgaagtggg ggtggaggtt
    23881 cgcgaggcgt ttatggcagt agacgctaaa gcaagtgcag ctttcattca gcatggtgat
    23941 aagtatctgg cggatattta tcagcttgcc cggcagcgtc tggcgaacgt gggtgttgag
    24001 caaattttcg gcggcgaccg ttgtacatat acggaaaatg agactttctt ctcttatcgt
    24061 cgcgacaaga ccaccggtcg tatggcaagt ttcatttggc tgatataacc taaagaatca
    24121 agacgatccg gtacgcgtga ttttcttttc acattaatct ggtcaataac cttgaataat
    24181 tgagggatga cctcatttaa tctccagtag caactttgat ccgttatggg aggagttatg
    24241 cgtctggatc gtcttactaa taaattccag cttgctcttg ccgatgccca atcacttgca
    24301 ctcgggcacg acaaccaatt tatcgaacca cttcatttaa tgagcgccct gctgaatcag
    24361 gaagggggtt cggttagtcc tttattaaca tccgctggca taaatgctgg ccagttgcgc
    24421 acagatatca atcaggcatt aaatcgttta ccgcaggttg aaggtactgg tggtgatgtc
    24481 cagccatcac aggatctggt gcgcgttctt aatctttgcg acaagctggc gcaaaaacgt
    24541 ggtgataact ttatctcgtc agaactgttc gttctggcgg cacttgagtc tcgcggcacg
    24601 ctggccgaca tcctgaaagc agcaggggcg accaccgcca acattactca agcgattgaa
    24661 caaatgcgtg gaggtgaaag cgtgaacgat caaggtgctg aagaccaacg tcaggctttg
    24721 aaaaaatata ccatcgacct taccgaacga gccgaacagg gcaaactcga tccggtgatt
    24781 ggtcgtgatg aagaaattcg ccgtaccatt caggtgctgc aacgtcgtac taaaaataac
    24841 ccggtactga ttggtgaacc cggcgtcggt aaaactgcca tcgttgaagg tctggcgcag
    24901 cgtattatca acggcgaagt gccggaagga ttgaaaggcc gccgcgtact ggcgctggat
    24961 atgggcgcgc tggtggctgg ggcgaaatat cgcggtgagt ttgaagagcg tttaaaaggc
    25021 gtgcttaacg atcttgccaa acaggaaggc aacgtcatcc tgtttatcga cgaattacat
    25081 accatggtcg gcgcgggtaa agccgatggc gcaatggacg ccggaaacat gctgaaaccg
    25141 gcgctggcgc gtggtgaatt gcactgcgta ggtgccacga cgcttgacga atatcgccag
    25201 tacatcgaaa aagatgctgc gctggaacgt cgtttccaga aagtgtttgt tgccgagcct
    25261 tctgttgaag ataccattgc gattctgcgt ggcctgaaag aacgttacga attgcaccac
    25321 catgtgcaaa ttactgaccc ggcaattgtt gcagcggcga cgttgtctca tcgctacatt
    25381 gctgaccgtc agctgccgga taaagccatc gacctgatcg atgaagcagc atccagcatt
    25441 cgtatgcaga ttgactcaaa accagaagaa ctcgaccgac tcgatcgtcg tatcatccag
    25501 ctcaaactgg aacaacaggc gttaatgaaa gagtctgatg aagccagtaa aaaacgcctg
    25561 gatatgctca acgaagaact gagcgacaaa gaacgtcagt actccgagtt agaagaagag
    25621 tggaaagcag agaaggcatc gctttctggt acgcagacca ttaaagcgga actggaacag
    25681 gcgaaaatcg ctattgaaca ggctcgccgt gtgggggacc tggcgcggat gtctgaactg
    25741 caatacggca aaatcccgga actggaaaag caactggaag ccgcaacgca gctcgaaggc
    25801 aaaactatgc gtctgttgcg taataaagtg accgacgccg aaattgctga agtgctggcg
    25861 cgttggacgg ggattccggt ttctcgcatg atggaaagcg agcgcgaaaa actgctgcgt
    25921 atggagcaag aactgcacca tcgcgtcatt ggtcagaacg aagcggttga tgcggtatct
    25981 aacgctattc gccgtagccg tgcggggttg gcggatccaa atcgcccgat tggttcattc
    26041 ctgttcctcg gcccaacggg tgtggggaaa actgagcttt gtaaggcgct ggcgaacttt
    26101 atgtttgata gcgacgaggc gatggtccgt atcgatatgt ccgagtttat ggagaaacac
    26161 tcggtgtctc gtctggttgg tgcgcctccg ggatatgtcg gttatgaaga aggtggctac
    26221 ctgaccgaag ctgtgcgtcg tcgtccgtat tccgtcatcc tgctggatga agtggaaaaa
    26281 gcgcatccgg atgtcttcaa cattctgttg caggtactgg atgatggtcg tctgactgac
    26341 gggcagggga gaacggtcga cttccgtaat acggttgtca ttatgacctc taacctcggt
    26401 tccgatttga ttcaggaacg cttcggtgaa ctggattatg cgcacatgaa agagctggtg
    26461 ctcggtgtgg taagccataa cttccgtccg gaattcatta accgtattga tgaagtggtg
    26521 gtcttccatc cgctgggtga acagcacatt gcctcgattg cgcagattca gttgaaacgt
    26581 ctgtacaaac gtctggaaga acgtggttat gaaatccaca tttctgacga ggcgctgaaa
    26641 ctgctgagcg agaacggtta cgatccagtc tatggtgcac gtcctttgaa acgtgctatc
    26701 cagcagcaga tcgaaaaccc gctggcacag caaatactgt ctggtgaatt ggttccgggt
    26761 aaagtgattc gcctggaagt taatgaagac cggattgtcg ccgtccagta aatgataaaa
    26821 cgagcccttc ggggctcgtt tttgtctata agttagacgg aaaagactat atttaagatg
    26881 ttttgcctga aaaatgagcg aacgataaag tttttatatt tttcgcttgt caggccggaa
    26941 taactcccta taatgcgcca ccactgacac ggaacaacgg caaacacgcc gccgggtcag
    27001 cggggttctc ctgagaattc cggcagagaa agcaaaaa
//
LOCUS       DAZGSP010000069        26766 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZGSP010000069 DAZGSP010000000
VERSION     DAZGSP010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.069"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(57..965)
                     /gene="melR"
                     /locus_tag="ACHK74_003176"
     CDS             complement(57..965)
                     /gene="melR"
                     /locus_tag="ACHK74_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFP0221943.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            1248..2603
                     /gene="melA"
                     /locus_tag="ACHK74_003177"
     CDS             1248..2603
                     /gene="melA"
                     /locus_tag="ACHK74_003177"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP0221944.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALHDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2706..4127
                     /gene="melB"
                     /locus_tag="ACHK74_003178"
     CDS             2706..4127
                     /gene="melB"
                     /locus_tag="ACHK74_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFP0221945.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(4266..4895)
                     /gene="yjdF"
                     /locus_tag="ACHK74_003179"
     CDS             complement(4266..4895)
                     /gene="yjdF"
                     /locus_tag="ACHK74_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFP0221946.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(5018..6664)
                     /gene="fumB"
                     /locus_tag="ACHK74_003180"
     CDS             complement(5018..6664)
                     /gene="fumB"
                     /locus_tag="ACHK74_003180"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP0221947.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYFEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(6742..8082)
                     /gene="dcuB"
                     /locus_tag="ACHK74_003181"
     CDS             complement(6742..8082)
                     /gene="dcuB"
                     /locus_tag="ACHK74_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFP0221948.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(8653..9372)
                     /gene="dcuR"
                     /locus_tag="ACHK74_003182"
     CDS             complement(8653..9372)
                     /gene="dcuR"
                     /locus_tag="ACHK74_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFP0221949.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(9369..11000)
                     /gene="dcuS"
                     /locus_tag="ACHK74_003183"
     CDS             complement(9369..11000)
                     /gene="dcuS"
                     /locus_tag="ACHK74_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP0221950.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            11181..11411
                     /gene="yjdI"
                     /locus_tag="ACHK74_003184"
     CDS             11181..11411
                     /gene="yjdI"
                     /locus_tag="ACHK74_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFP0221951.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            11423..11695
                     /gene="yjdJ"
                     /locus_tag="ACHK74_003185"
     CDS             11423..11695
                     /gene="yjdJ"
                     /locus_tag="ACHK74_003185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0221952.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            11922..12218
                     /gene="ghoS"
                     /locus_tag="ACHK74_003186"
     CDS             11922..12218
                     /gene="ghoS"
                     /locus_tag="ACHK74_003186"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP0221953.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            12246..12419
                     /gene="ghoT"
                     /locus_tag="ACHK74_003187"
     CDS             12246..12419
                     /gene="ghoT"
                     /locus_tag="ACHK74_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFP0221954.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(12538..14055)
                     /gene="lysS"
                     /locus_tag="ACHK74_003188"
     CDS             complement(12538..14055)
                     /gene="lysS"
                     /locus_tag="ACHK74_003188"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP0221955.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(14292..15749)
                     /gene="dtpC"
                     /locus_tag="ACHK74_003189"
     CDS             complement(14292..15749)
                     /gene="dtpC"
                     /locus_tag="ACHK74_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFP0221956.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMAVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(15808..17955)
                     /gene="cadA"
                     /locus_tag="ACHK74_003190"
     CDS             complement(15808..17955)
                     /gene="cadA"
                     /locus_tag="ACHK74_003190"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFP0221957.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQIRF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(18035..19369)
                     /gene="cadB"
                     /locus_tag="ACHK74_003191"
     CDS             complement(18035..19369)
                     /gene="cadB"
                     /locus_tag="ACHK74_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFP0221958.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(19735..21273)
                     /gene="cadC"
                     /locus_tag="ACHK74_003192"
     CDS             complement(19735..21273)
                     /gene="cadC"
                     /locus_tag="ACHK74_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFP0221959.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPDTDSPSHSLTIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(21533..21691)
                     /locus_tag="ACHK74_003193"
                     /pseudo
     CDS             complement(21533..21691)
                     /locus_tag="ACHK74_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(22154..22480)
                     /locus_tag="ACHK74_003194"
     CDS             complement(22154..22480)
                     /locus_tag="ACHK74_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0221960.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            complement(22477..22740)
                     /locus_tag="ACHK74_003195"
     CDS             complement(22477..22740)
                     /locus_tag="ACHK74_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFP0221961.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            complement(22812..23678)
                     /locus_tag="ACHK74_003196"
     CDS             complement(22812..23678)
                     /locus_tag="ACHK74_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP0221962.1"
                     /translation="MSGITISRPEVVNGHTDVICSTSIGHILAVRKTTLMQIDTLIRQ
                     LTEISAMTESIGGKTAPDWAMKQNFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISAANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHFITGQQADRLADLERMLHLFRGKPIPDNR
                     ENITLRLDDHIQSVQGKEDYEDEMFSIKYFKKGSVHITFRKPELVDRLNDIIARHYPE
                     ALASTVNKSRKA"
     gene            complement(23763..23960)
                     /locus_tag="ACHK74_003197"
     CDS             complement(23763..23960)
                     /locus_tag="ACHK74_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP0221963.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(23972..24460)
                     /locus_tag="ACHK74_003198"
     CDS             complement(23972..24460)
                     /locus_tag="ACHK74_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP0221964.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGIELAELINKVAENGYS
                     LHVVDDSDQQSTCTPPPLTTLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKMFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(24457..24834)
                     /locus_tag="ACHK74_003199"
     CDS             complement(24457..24834)
                     /locus_tag="ACHK74_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP0221965.1"
                     /translation="MKTLSDTHVREASRCPSPVTIWQTLLIRLLNQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRMVNNITQGKHPEAQQ"
     gene            complement(24881..25255)
                     /locus_tag="ACHK74_003200"
     CDS             complement(24881..25255)
                     /locus_tag="ACHK74_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP0221966.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRNQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPAQAPATTS"
     gene            complement(25418..25639)
                     /locus_tag="ACHK74_003201"
     CDS             complement(25418..25639)
                     /locus_tag="ACHK74_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP0221967.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWQGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(25702..26178)
                     /locus_tag="ACHK74_003202"
     CDS             complement(25702..26178)
                     /locus_tag="ACHK74_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP0221968.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(26194..26679)
                     /locus_tag="ACHK74_003203"
     CDS             complement(26194..26679)
                     /locus_tag="ACHK74_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0221969.1"
                     /translation="MTTVSHNFTTSSVSVTTASGNNQSQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCENYCDGIWNLYTLNNGGAFMAPEPDDDDGETWVLFNA
                     MNGNRAEMSPEAAGIAACLMAYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
BASE COUNT         6811 a         6316 c         6637 g         7002 t
ORIGIN      
        1 ttcactgctt attatcgacg gctaaactat ttttttggct gatactgata tcgtctttag
       61 ccgggaaacg tctggcggcg ctgttggcta agtttgcggt attgttgcgg cgacatgccg
      121 acatatttgc cgaacgtgct gtaaaaacga ctacttgaac gaaagcctgc cgtcagggca
      181 atatcgagaa tacttttatc ggtatcgctc agtaacgcgc gaacgtggtt gatgcgcatc
      241 gcggtaatgt actgtttcat cgtcaattgc atgacccgct ggaatatccc cattgcatag
      301 ttggcgttaa gtttgacgtg ctcagccaca tcgttgatgg tcagcgcctg atcatagttt
      361 tcggcaataa agcccagcat ctggctaaca taaaattgcg catggcgcga gacgctgttt
      421 ttgtgtgtgc gcgaggtttt attgaccaga atcggttccc agccagagag gctaaatcgc
      481 ttgagcatca ggccaatttc atcaatggcg agctggcgaa tttgctcgtt cggactgttt
      541 aattcctgct gccagcggcg cacttcaaac gggctaagtt gctgtgtggc cagtgatttg
      601 atcaccatgc cgtgagtgac gtggttaatc aggtctttat ccagcggcca ggagagaaac
      661 agatgcatcg gcagattaaa aatcgccatg ctctgacagg ttccggtatc tgttagttgg
      721 tgcggtgtac aggcccagaa cagcgtgata tgaccctgat tgatattcac tttttcattg
      781 ttgatcaggt attccacatc gccatcgaaa ggcacattca cttcgacctg accatgccag
      841 tggctggtgg gcatgatatg cggtgcgcga aactcaatct ccatccgctg gtattccgaa
      901 tacagcgaca gcgggctgcg ggtctgtttt tcgtcgctgc tgcacataaa cgtatctgta
      961 ttcatagatg gctctctttc ctggaatatc agaattatgg caggagtgag ggaggatgac
     1021 tgcgagtggg agcacggttt tcaccctctt cccagagagg cgaggggagc gtccgagcat
     1081 catgaggccg aaaactctgc ttttcaggta acttattacc ataaactcag atttactgct
     1141 gcttcacgca ggatctgagt ttatgggaat gctcaacctg gaagccggag gttttctgca
     1201 gattcgcctg ccatgatgaa gttattcaag caagccagga gatctgcatg atgactgcac
     1261 ccaaaattac atttatcggc gctggttcga cgattttcgt taaaaatatt cttggtgatg
     1321 tgttccatcg cgaggcgctg aaaacggcgc atattgccct gatggacatt gatcccaccc
     1381 gcctggaaga gtcgcatatt gtggtgcgta agcttatgga ttcagcaggg gccagcggca
     1441 aaatcacctg ccacacccaa cagaaagaag ccttacatga tgccgatttt gtcgtggtgg
     1501 catttcagat tggcggttat gaaccttgca cggtgactga tttcgaggtc tgtaagcggc
     1561 atggtctgga acaaaccatt gccgatacgt tggggccggg cggtattatg cgcgcgctac
     1621 gtaccattcc gcatctgtgg caaatttgcg aggacatgac ggaagtctgc cccgatgcca
     1681 ccatgctcaa ctatgttaac ccaatggcga tgaatacctg ggcgatgtat gcccgctatc
     1741 cgcatatcaa acaggtcggg ctgtgccatt cggtgcaggg aacggcggaa gagctggcgc
     1801 gtgacctcaa tatcgaccca gctacgctgc gttaccgttg cgcaggtatc aaccatatgg
     1861 cgttttacct ggagctggag cgcaaaaccg ccgacggcag ttatgtgaat ctctacccgg
     1921 aactgctggc ggcttatgaa gcagggcagg caccgaagcc gaatattcat ggcaatactc
     1981 gctgccagaa tattgtgcgc tacgaaatgt tcaaaaagct gggctacttc gtcacggaat
     2041 cgtcagaaca ttttgctgag tacacaccgt ggtttattaa gccaggtcgt gaggatttga
     2101 ttgagcgtta taaagtaccg ctggatgagt acccgaaacg ctgcgtcgag cagctggcga
     2161 actggcataa agagctggag gagtataaaa acgcctcccg gattgatatt aaaccgtcac
     2221 gggaatatgc cagcacaatc atgaacgcta tctggactgg cgagccgagt gtgatttacg
     2281 gcaacgtccg taacgatggt ttgattgata acctgccaca aggatgttgc gtggaagtag
     2341 cctgtctggt tgatgctaat ggcattcagc cgaccaaagt cggtacgcta ccttcgcatc
     2401 tggccgccct gatgcaaacc aacatcaacg tacagacgct gctgaccgaa gctattctta
     2461 cggaaaatcg cgaccgtgtt taccacgccg cgatgatgga cccgcatact gctgccgtgc
     2521 tgggcattga tgaaatatat gctcttgttg acgacctgat tgccgcccac ggcgactggc
     2581 tgccaggctg gttgcaccgt taaaacgcga ctaaacgcta ctgcgccggg ggatttattc
     2641 cggcgcacac ctctgacgat accaataaca gaaggcgggc gttggtaaca gcgacccgat
     2701 accctatgag catttcaatg actacaaaac tcagttatgg atttggagcg ttcgggaagg
     2761 attttgcgat cggcattgtg tatatgtacc tcatgtatta ctacaccgat gtcgtcgggc
     2821 tgtctgtggg tttggtcggt actttgtttc tggtggcgag gatctgggat gctattaacg
     2881 atccgattat gggatggatt gtaaatgcta cgcgatcgcg atggggtaag ttcaaaccct
     2941 ggatcctgat cggtacgttg gcaaactctg taatcttatt tctcctcttt agtgcgcatc
     3001 tgtttgaagg tactactcag attgtctttg tttgcgtgac ctacatcctc tggggcatga
     3061 cttacaccat tatggatatt cccttctggt cgctggttcc aaccatcacg ctcgataaac
     3121 gtgagcgcga acaactggtt ccttatccgc gtttttttgc cagtctggca ggctttgtta
     3181 cggcaggtgt gacgctacca tttgttaatt atgtcggcgg tggcgatcgg ggatttggct
     3241 ttcagatgtt cactctggta ctgatcgcct tttttattgt ttcaaccatc atcactctgc
     3301 gcaatgtgca tgaagtcttt tcgtcagaca atcaaccgtc tgctgaagga agccatctga
     3361 cacttaaagc catcgttgcg ctaatttata aaaacgatca gctttcatgc ctcttgggta
     3421 tggctcttgc ttataatgta gccagcaaca ttattaccgg atttgctatc tattatttct
     3481 catatgttat cggtgatgcg gatttgttcc cctattatct gtcgtatgcg ggagctgcta
     3541 acctggtgac gttagtattc ttcccacgct tagttaaatc attatcccga cgcattttat
     3601 gggccggagc atctattctt ccggtgttaa gctgtggtgt tctcctgtta atggcattaa
     3661 tgagctatca caacgtcgtc ctcattgtga ttgcgggtat tttgctgaat gtgggaacgg
     3721 cgcttttctg ggtattacag gtcatcatgg tggcagatac cgttgattac ggtgaatata
     3781 aactgcacgt acgctgtgaa agtatcgctt actccgtgca gactatggtg gtgaagggcg
     3841 gttcagcctt tgcggctttt ttcattgcgg ttgtgttagg gatgattggc tatgtaccga
     3901 atgttgaaca gtctacgcaa gccctattag gtatgcagtt tattatgatt gctctaccaa
     3961 ctctgttttt catggtaacg ctgattctct acttccgttt ctatcgcctc aatggcgaca
     4021 cgctgcgcag gatccagatt catctgctgg ataaatatcg caaagtaccg cccgagcctg
     4081 ttcatgctga tattccggtc ggtgcagtga gtgatgtgaa agcctgacgt ggacaatagc
     4141 taacgaaaat ggcctgatgc gatatgttta tcaggccacg tcttaatgta atgctttgga
     4201 tttgcaaatt ttgtagaccg gataaggaat tcacgccgca tccggcatca acaaagcgca
     4261 cgttgttatc cggttatcaa gccaaagcgc cgtagctggc ggcaatgaaa gcgagcgagg
     4321 aatatcaccg tcgttaatgc gccaagcagc gcgcagaaca tatcggattg cgtgtcccac
     4381 tggtcgccct gggtgccgag aaaatcatcc gctccctggc ccattgccag cgccgcccac
     4441 cactcgatta attcatacat cgcgcttatc gccagcgcca cgcagcagac caggaacgcc
     4501 accattttac gtccgcgcac gtacatcccg cgaacgagaa tttctcgtgc caccagtgca
     4561 ggcaccagcc cctggaaaaa atgccccagc ttgtcatacg gattacggct caaccctaac
     4621 cattcctgca cctcaaaacc aacggggact ttcgcgtagg tgtattgtcc gccgaccatc
     4681 aggatgatgg cgtgaaagaa aatgagcgta tagagcagcg gcgttaacgg ataacgtttc
     4741 gcggtggcaa ggagtagcgg cacgacaata atcaccggtg tcacttccat cagccaggtg
     4801 agtttgtcat gggccgaaat gccggtataa atcaggatta acgttagcgc cagtgcgccg
     4861 gtgttaagaa ttaacggctt gagtgtgcgg gtcatggtga gttcgcaagt cagggaaaat
     4921 actgactatt caccagcaag cgtgaaatta caatcgcaaa agaggtggcc cagggggatc
     4981 acctggcagc atgctgccag gcgctgggcc gaagaggtta cttagtgcag ttcgcgcact
     5041 gtttgttgac gatctgctgg aagaagtcgt tacctttgtc atcgaccagg ataaacgccg
     5101 ggaaatcttc tacttcgatt ttccagatag cttccatacc cagctccgga taagcgacgc
     5161 attccagatg cttgatgctc tgctgcgcca gtaccgccgc cggaccgccg atgctaccga
     5221 ggtagaagcc gccgtgttta tgacacgcgt cggtaacctg ctgactgcgg ttacctttcg
     5281 ctagcatgat catgctgccg ccgtgggatt gcagcagatc cacgtaggag tccatacggc
     5341 ctgcggtggt tgggccaagt gaacctgatg gataaccggc aggggttttc gccggacccg
     5401 cgtagtagat cgggtgatct ttgatgtact gcggaagttc tttaccggcg tcaatcagct
     5461 ctttcagctt ggcgtgtgca atatctcggc ccacgataat ggtgccggtg agcgacaaac
     5521 gagtggatac cgggtattgc gaaagctggg cgaggatctc tttcatcggg cggttaaggt
     5581 caactttcac cgcttcgcct tcaccggcct ggcgcagttc ttgtggaatg tactggcctg
     5641 ggttgtgttc cagtttttcg atccagatac cttcgcggtt gattttcgct ttaatgttac
     5701 ggtcagcgga gcaggagacg cccatgccga ccgggcagga tgcgccgtga cgtggcagac
     5761 ggataacgcg aatgtcgtgc gcgaagtatt taccgccaaa ctgcgcgcca agaccgagtt
     5821 tctgggcctc ttccagcagt tcctgttcca gctggacatc gcggaacgcc tgaccatgtt
     5881 cgttcccttc cgtcggcagt tcatcgtaat agtgagcgct tgctaacttg acggttttca
     5941 ggttggtttc cgcagacgta ccgccaatca caaacgcgat atggtacggc gggcaggctg
     6001 cagtaccgag ggtacgcatt ttctcgacga ggaagttttt cagtttgccg ggagtcagca
     6061 gggctttggt ttcctggtag agatacgttt tgttggcaga gccgccgcct ttcgcaacgc
     6121 aaaggaattt gtactcatcg ccatctaccg cgtacaggtc gatttgcgca ggcaggttag
     6181 tgccggtgtt gacctctttg tacatgtcca gcgccgcatt ctgtgaatag cgcaggttat
     6241 cttcgaaata ggtgttatag acgcctttcg acagcgcttc ttcatcaccg ccaccggtcc
     6301 acacgcgctg gcctttttta ccgacgatga tcgcggtgcc ggtatcctgg caggtcggca
     6361 gcacgccttt ggcggcgatt tcggagtttc ttaagaattg cagcgccacg tacttgtcgt
     6421 tttcgctggc ttctggatcg tgaagaatag ccgcaacctg tttctggtgt gccggacgga
     6481 gcatgaaaga agcgtcgtga aaggcttgct gcgccagtag ggtcagggct tctggttcca
     6541 ctttcaggat ggtttcgccg tcgaagtcgg caacgctaac gtaatcggaa gtgagtagat
     6601 agtattcggt attgtctttc cccatcggga aaggtgcctg gtagataaag ggtttgtttg
     6661 acatagcttc cagcctgtaa ctctgtattt gttattcgaa aatggcgtgc cgcgtagtca
     6721 cgggcggcac gcaaagtgca tttataagaa cccgtacatc gcggcgaaga tccagccgaa
     6781 gacgcacgat acgctcacac caatcaaccc cggcagaata aaactgtggt tgatgacgaa
     6841 gcgaccgatg tgggtggtgc cggaacggtc aaactgaatc gctgccagat cgctcggata
     6901 agtcggcagg atgtaataac cgtagcaagc cggtgctgaa gccacgatgt atgccggatc
     6961 aacgccgatc gccagcgcga ccggaacaat cgccgccagc gccgcagcct gagagtttac
     7021 aaacttggaa accagcagca gaacaatggc atacgcccac ggatactctt tcaccatttc
     7081 acccagtacg ccctgaattt cagacatatg cgcaccgaac atggtttctg ccatccatgc
     7141 gataccgtac accgccacga tggcgatcat accggaacgg aagacttcgt tttttgagat
     7201 agacgcggga ttggttttgg tcaggataat aatcagcgcc ccggtcagca gcataaacat
     7261 ctgaataacc agtaccatcg acagcggttt gccgccgaag gatggacgca ggtccgaatc
     7321 agcaccaaga agtgcgacta cagcgattgc accgaggaaa atccacattg ccagccagtt
     7381 gcttttcggc agttttttat cgagcagcgt cgcggtatca ccgtaaacat actcacggtt
     7441 ttccggtacg gagatgaatt tctggaactc ttcgtcttta tccagatctt taccgcggaa
     7501 ccagctgaag ataccgatcg ccaggatacc gattaacgtc gatggaatgg tgattgccag
     7561 caggtcgagg aactcaagat ggcgaccatc aaaggtgaca ttacccagca ttgcaaccag
     7621 agacacgacc gcaaccgaca ccggactggc gataatcccc atctgtgcac cgatagaact
     7681 tgccgccatc ggacgttccg gacggatgtt gttcttaatg gcgacgtcgt agatgatcgg
     7741 cagaatggtg taaaccacat gacccgtacc gcaaagaatg gtcagtgtac aggtcacaaa
     7801 cggcgcgaca attgagacat atttcgggtt gcggcgcagc aacttctcgg caatttgcag
     7861 catgacatca agaccgcccg aagcttgcaa ggtcgccgat gccgccacca ccgcaatgat
     7921 aaccagcatg acatcaactg gtggtttacc tggctgaagg tggaagacga agaccagaat
     7981 gaccagaccg ataccgccta ataaacccag cgcgatacca ccctttctgg caccataaaa
     8041 cagacatatc agtattatga taagttggat agtaaataac atgtgtgaac cctcgcgata
     8101 atcctattta aatttttgct gaatagatca cagtcacgtt ctgttttgta tgaactgttt
     8161 tcagagcctg actaaatatc cgtttggtcg ttaccggctt tagcaaatac ctcacagtaa
     8221 atattggctg attgaagcag cggcaaattt atgcactgtg ataagcggct ttttcagaga
     8281 gaaaaactct ctggcgatgg gtttatagtt tgtactgcgc agatgttaat gttttgtttg
     8341 ctgttatctt attgatattt atcggtctat tttttgttct cagggcagat atcattaggt
     8401 ttaaagatta atctgatcta cccatttgtg ggtaaaaata cacataatgc gggtgacata
     8461 atagttaatt aacttttgtt agcgttttga aattaaaaac accgttcacc tgaagagata
     8521 ttaattttta gcgatgatgg agggataatt atatttgatc tggcacaagt tttactgatg
     8581 aaggatgtaa cttgtgccag gggtattttg cattacggta ataattatta cttacagata
     8641 accagcgatc cgttattggc aatattgttt cagtagtgag tagtgttctg cctgaatacg
     8701 gtaacggtaa accggacgcc ccgtgacgcc ataatggata ctggtgaaca agatgtggca
     8761 gttgaccagc cagatgaggt atttacggca ggaaacacgc gagatgttaa cctcgttagc
     8821 cagctcgtcg gttgaaaact catagtcctg atgcgcgtca atccactggc acagcgtgcg
     8881 taacgtctgc ggcgttaagc cttttggcaa gcgacgagga tcctgttcgt tggagctgct
     8941 gccgtggatt agctgatcaa gctcggcctg gtcataatac tgatgttttt ccagcgccat
     9001 tttcttttgc cgccagccgg tgagcgcctc ttcaaagcgg gaagcctgga agggtttgat
     9061 caggtaatcc acgacaccgt aatgcagcga atctttaatg gttgccgcat cggctgcgga
     9121 ggagatgaca atcacatcac ttttgcaacg cgcgttatgc aggacaggca gtaaatcgag
     9181 cccgttctct ttttgcatat agatatcgag caatatcagg tcgataggcg tatcgctatt
     9241 gaagataatc tctttggctt tctccagcgt cgaggctgtt ccacagcatt gaaagcctgg
     9301 gatttgtgct acgtatcggc gattcagctc cgcgaccatt gcgtcgtcat cgataattaa
     9361 tacattgatc atctgttcga cctctccccg tcccagggta tctggacaaa aaattgtgtg
     9421 aaaatcccgg gttccgattc cacggcgatg ctgccgccga gattttctac ctgttgtttg
     9481 acaagtgcta aaccgacgcc tcgctcgctt ccttttgtcg agacaccttt gtcaaaaatg
     9541 tgatcgattt tgtcgggtgc gatccccggt ccatcatcgt taacttcgca gtgcagccag
     9601 ccgtgacggt agtgcaatgt tacgctaatt tcgccaccgg gttccggccc taatgcttcc
     9661 agcgcgtttt ctatcagatt tcccaatgtg gtaatcagcg tcgcgacctg gtcctcactg
     9721 ccgctgtctg gcagctggct ttcactgttt aaaatcagcg tatggcctaa atcggtcgcg
     9781 cgattaatct tgctgattaa aaaaccagcg ataaccggag atttgatctt acccagcaga
     9841 gagccaatct cttcctgata gttattggct gttttgagaa tgtaatcttc caactgctta
     9901 taactcttca gatgcaataa tccgagaatc acatgcaatt tattcataaa ttcgtgggat
     9961 cgttcacgaa gtgcatcagc atagttgacc agaccgtcga gtcgctgcat cagtttacga
    10021 acttcagttt tgtccctgaa ggttgaaatg gcaccgatga taacgccatt actgcgcacc
    10081 ggaacggtgt tgatcagtaa tagccggtct ttaatcgtaa tctcttcgtc gcggcgcggc
    10141 gtaccgtcgc gtaacacttc cgagacatct accacctgtg accatgagtg gctgagcgtc
    10201 gacagtttct catcgtcctg cgacttacgg taattcagca attcttgtgc ggcatcgttg
    10261 atcagcgtga cctcgccgcg atcgtccacg gcaacgacgc cttctttgat agactgcaac
    10321 atggcctggc gctgctcaaa cagcgtggag atttcgtagg gttccaggcc gaaaaggatt
    10381 tttttcagta ccttaaccag aatgcaggtg ccaatcagtc cgaccagcat gccaaataat
    10441 accgaccaga taatgctcca gcgactgtca ttgatttgtt gggtcacgcg gcttaactca
    10501 aggccgatcg ccaccacgcc aatttgttta tgattttcat cgtagatggg ggtaaatacg
    10561 cgtaaagcct gcgccagaaa accgcgattg atagcgacat tttctttgcc attcagcgct
    10621 ttaaggatgt catcaccttt aaatggctga ccaatacgct gggcttcagg atgcgagtag
    10681 cgaagacttt gcatatcggt aacgacaata aacagcagat cgttgcgttt gcgcacggct
    10741 tccgcgatgg cctggattcc gctctcctgc ggttttttct gcaagccctg acggatttcc
    10801 ggcgagtcgg cgagggtacg tgccactgcc agtgccttgt tggccagccc gtctcgcgtc
    10861 atatcactga tttgcgagaa gtaaatcaga tgcaccacca atagcaccga gaacagtacc
    10921 gcgctgacca ttaagatcac tgtggtactc aatttcatcg gacgtttgcg taacatgcgg
    10981 tagggcaatg aatgtctcat cagcttcctt gtgtgacaaa tttcttaagc attatctctg
    11041 atgaggcggg taattcaaag ggagtaagaa tgattggcta tataggggaa gagactctgg
    11101 caacggaaac tgccagtgct gtatgaagat tccagggcta tgcttatagc gataatcata
    11161 ctgatgagag agggaaagtc atggatcagg cgctactgga cgagggttat cgctgttata
    11221 ccggcgaaaa gatcgatgtc tatttcaaca ctgcgatatg tcagcattct ggcaattgcg
    11281 tacgtggcaa cggcaagtta tttaatctca aacgaaagcc gtggatcatg ccggatgaag
    11341 tcgacgtcgt cactgtggtt aaagtgattg atacgtgccc gagcggcgcg ctgaaatacc
    11401 gtcataaata agcgagggta aaatggaaat acgcgaaggc cacaataaat tttacattaa
    11461 tgacgaacaa ggcaagcaaa tcgctgaaat tgtctttgtg ccgaccggag agaatttagc
    11521 gattatcgaa cataccgatg tcgatgaaag cctgaaaggg caggggattg gtaaacagct
    11581 ggttgcgaaa gtcgtggaaa aaatgcgtcg ggaaaaacga aaaattatcc cactatgccc
    11641 atttgcgaaa cacgaatttg ataaaacgcg ggagtatgat gatattcgca gttgatggga
    11701 gagtacagag tcacgatatt tttcatgctc tccgcggagt gatgcaggag agcatctgaa
    11761 gggtaggggg atgcacaaag aatgggcaga gagcgcgttt ttttgtccca agtcatcccc
    11821 tttactgagc aaaaaaaaga atatctccta tatgagaatc atcaatcggg gttaataatt
    11881 tttgcgtccc cagagcattc aatattgata ggagtcatat tatggaaggt aaaaacaagt
    11941 tcaatactta tgttgtttct tttgattatc catcatctta ttcctcagtg ttcttaaggt
    12001 taagatcatt gatgtatgat atgaatttct cttctatcgt ggctgatgaa tatgggatac
    12061 cacgacaatt gaatgaaaat tccttcgcaa taacgacatc gttagccgca agtgaaatcg
    12121 aagatttaat caggcttaaa tgcttagact taccggatat tgattttgac ctcaacatta
    12181 tgacagttga tgactatttc cgtcagtttt acaagtagcc aggaaggata agaagaacat
    12241 aaaaaatggc actattctct aaaatattaa ttttttatgt gattggtgtg aacatatcct
    12301 ttgtcattat ctggtttatc tcacatgaga aaacacatat tcgtttactt agtgcattcc
    12361 tggtcggaat aacctggcca atgagtctgc ctgtggcatt acttttttct ctcttttagg
    12421 agctgtagtt gagtggaccc gctggttatc gtgaaacgaa gcgtctctcc tcaaaactgg
    12481 atttgcggaa cgacgaacga agcagtcgcc tgactgcttc gttcattaaa gtgaaattta
    12541 tttctgtggg cgcatcgccg ggaagagaat aacgtcgcga atagtatgac tgtttgtaaa
    12601 cagcataatc attcggtcga taccaatacc cagaccagcg gttggcggca gaccatattc
    12661 cagcgcagtc acgtaatctt cgtcatagaa catggcttcg tcgtcacctg cagctttagc
    12721 gttaacctgt tcctggaaac gttcagcctg atcttctgcg tcgtttaatt cgctaaaacc
    12781 attaccgatt tcacgaccac cgatgaagaa ttcaaaacgg tcggtgattt ctgggttaac
    12841 atcattacgg cgtgccagcg gtgacacttc tgccggatat tccgtaataa aggttggctg
    12901 aatcagatgt gcttctgcca cttcatcaaa gatctccgtg acaatacgtc ccagccccca
    12961 gcttttctct accgtaatac cgatagattc agctaatgct ttagcagcat caaaattatc
    13021 caggtcggcc atatcggttt ctggacgata ttttttgatt gcttcgcgca tggtgagttt
    13081 ttcaaacggt ttgccgaaat caaacacatg ctcgccataa gtgactttag tggtacccag
    13141 aacctcttgt gccagggtgc ggaacaggga ctctgtcagt tcaatcaaat cgtggtaatc
    13201 cgcatacgcc atgtagagtt ccatcattgt gaactcagga ttatggcgaa cagaaatacc
    13261 ttcattacgg aagttacggt tgatttcgaa tacccgttca aaaccgccta caaccagacg
    13321 tttcagatac agctccggcg cgatacgcag gtacatatct aaatccagag cgttatgatg
    13381 ggtaataaac gggcgagcag atgccccacc tggaattacc tgcatcatcg gggtttctac
    13441 ttccataaag ccgcgcgcga ccatgaactg gcggatagcg gccagaattt ttgaacggac
    13501 aacaaacgtt tgacgggatt tatcgttagc gatgaggtcc agataacgtt gacgataacg
    13561 gacttcctga tcctgcagac catggaattt atctggtaaa ggacgtagtg cttttgtcag
    13621 caggcgcaac tcagtacagt gaatggaaag ctcaccggtt tgcgtcttaa acagcgtacc
    13681 gcgggcaccg ataatgtcac ccagatccca ttttttaaac tgatcgttat aaacaccttc
    13741 tggcaggcta tctcttgcaa cgtacagttg aatacggcca ccgacatcct gcaacgttac
    13801 aaaggaggct ttccccatga tacgacgggt catcattcgg ccagcaaccg agacttcaat
    13861 gtttaaggat tccagttcct ggttatcctt cgcatcaaac tcttcgtgca gctggtcaga
    13921 ggtatggtcg cggcgaaaat cattgggaaa cgccacacct tgctgacgta gtgccgccag
    13981 tttttcgcgg cgatttctca gttcatcgtt aaaatcaata gcctcattgg ctccccgtgt
    14041 ttcttgttca gacattttgg ttcctctaaa tccagctttc aatttagcgt agataaagag
    14101 acagatcggt acactgtatt atctgcctcg actattaata actcagttta tcttatcgaa
    14161 actaacgctt acctcaagtt gatatcacta ataaataacc atcaaaatgg tttaaatgac
    14221 ttattaatct agatttgcta gtgttataaa ctgaaagtta atttattcat aaccaattgt
    14281 ttttacgaca gttaatcgtt gctctcctgt atcatattcg tcggcgtgct aaacagaaaa
    14341 cgggtagcaa aggccaatac gacgatgatc gcgacacaag ccaacgtcca ctctcccatc
    14401 tgagaaaaga atcgctggta agctgcaatt gccataccgc taatttgcga ctctgtcgtc
    14461 tgctgtgcca cgacgcctgc cagccagttg gcgaccgcgc ctgttgccag catataaata
    14521 ccggttaata cgccagacat tttcagacgc gtgatttgcg caatcgccac cgggtcaata
    14581 aagagttcgg caaagcccat tagcgccagc ccggatatca tcacggccat tgacgcttga
    14641 ccgccagccg ctgcatgtcg ggcatcaaat gccaacaaca taaagccaca agccatcagc
    14701 agtaagccaa aggcaaactt cagccagacg cgcaatgttg agttgccgcg gctttctggc
    14761 gacgccagcc aggccagtac aaccccagcg agcatcactg caattgcatt caccgactgg
    14821 aatagtgctg taggtacttc aatattgaat gcctgacgat tcacaaagcg atcgataaac
    14881 aagctgatgg tactgccgcc ctgttgtgcc agtacccaga acaacgtccc gacaaacatc
    14941 aacaatacaa tttgccaaag agcacggcgg tgttcgggga atttgatcat catgcgggca
    15001 atgatttgtg cggcaatgag gcaaacgatc gccagcaaat atccggacca gtcgttctcc
    15061 agcagcaggg taaaaaatac tggggctaaa cagagcatca ccactaacca gctccatact
    15121 ggtaaggcaa atttcacgct agtgagcgct tttttatcca tactacgcgt ggactggaaa
    15181 tgacgatgcc cgcttaagaa aatcaacaaa ccgataaaca tgccgccacc cgcaagggca
    15241 aagccaacat gccatccata ccactgagca gccaggccgc aggcgattgg ggcggcgata
    15301 gaaccgatat tgcccgcagc atacagcagc gaaaaaccgc catcacgtcg atgatcgttc
    15361 tcgtcgtaga gctcgccaag caaacagctg atgtttgatt tgaataaacc gtagccacaa
    15421 ataatgattg ccagcgccag atacaggcta aaggttgaat ttgtatcaat gcccagcacc
    15481 acatggccaa gggtcattaa cagcgcgccg gcaatcactg cagtgcggtt gccgagcagg
    15541 cggtcggcaa gccagccgcc gagaataggg gtaacgtaaa ccagagaagc atatgcgctg
    15601 aacaggctga tggcatggtt atcatcaaaa ccaagctgat gggtgagata gagaatgagt
    15661 aaggcacgca tgccgtaaaa actgaagtac tcccagattt ggatcgccac gatatagtat
    15721 atcgcgcgcg gctgtgaggg tgttttcatg tgttctcctt atgagcaaaa aagggaagtg
    15781 gcaagccact tcccttgtac gagctaatta ttttttgctt tcttctttca ataccttaac
    15841 ggtatagcgg ccatcagcct gacggtatgc accgtgaata tcggtttcaa agcccggata
    15901 gtgagcgccg atttcacaca gcatctgcag gaactccaga accggacggc tttcttcggt
    15961 gatcatttca cccggcatta ccagaggaac tcccggcgga tacggaagga tcatattggc
    16021 gttaatacga cctaccattt cgtcgaggta aacttcttcg gtcataccgt gcagctcttt
    16081 ctggaatgca gcatacggag tcattaccat cgtcggcagc acttcaaatg cgcggtacat
    16141 cagatccggc agattgtggt gaacaatcag tttgtggata ttctgagcca gttcctgaat
    16201 acgcatgttt tcatagaatt caggatcttc acgatacaga gacggcagca tgtttttcac
    16261 acgcaggttc aggtcgaacg cacgtttgaa gtcagtcaga gcacgcagca ggctcagtgc
    16321 tttggtctta tcgataccga tgctgaacag gaacagcagg ttatacggac cggttttctc
    16381 aacaacgatg ccatgttcgt cgaggtattt cgccacgatg ctggccggaa taccaaagtc
    16441 gctcatggtg ccgtcttttt ccatccccgg agtcagcagg gtgactttga tcgggtcaag
    16501 atacatgtgc tcgttatcga tgtttttgaa gccgtgccag gtgctgtcag aacgcagcgg
    16561 ccagcattca gtggtatcga tatgatccgg ctgccataca tcaaagaacc agccatcaga
    16621 ttccgttctc agacgtttga tctctttacg gaatttgatc gcacgttcaa tggaaccgtt
    16681 gatcagacgc ttaccagcat tgcctttcat catcgccgct gcggtttcag tggacgccac
    16741 gataccgtag tgcggagaag tggtggtgtg catcatgtag gcttcgttaa aggtttcttc
    16801 gtttacgtca cctttaacgt ggatcatgga agcctgagag aacgccgcca gcagtttgtg
    16861 agtggactgg gtttcgtaaa tcactttccc ttctacacgg ccaccgctca taccgcattt
    16921 accttcgtaa atcggtgaga agttggtgta aggcacccac gcggagtcaa agtggatgga
    16981 tttcacatcc agtgttttct tgataaagtc ggtgttgtac agcagaccat cataggtaga
    17041 gttggtaatt acagcatgta ccggccaggt tgcgtttggt gtttctttca cgcgcttagc
    17101 aatggtagcg tgctggaatt cactctgtgg gataccacca agaataccgt aagcgttacg
    17161 ggtcgggcgg aaatagattg gcgtaacatc gctcatcatc atcaggtggg tcagcgattt
    17221 gtggcagtta cggtcaatca gaatggtgct gcctgctgga gcagagtaca taccaacaat
    17281 tttgttcgca gtggaagtac cgttggtcac catgtagctg cggtctgcgt taaagacgcg
    17341 agcgatatac tgttctgctt ctttgtgtgg accactgtga tccagcagag aacccagttc
    17401 agatactgaa atggaaatat cagatttcat ggtgttcgga ccaaagaaat catagaacag
    17461 gctacctacc gggcttttct ggaatgcagt accgcccatg tgaccaggag tacagaaagt
    17521 atatttacct tcacgaacat atttaaacag tgctttagtc agcggaggca gaatagtgtt
    17581 gatatattcg tcagtggtct gcttgatctt attagcaata tcttcagcag cacccagcgc
    17641 atattcaaag aagcgaatct gtaaacgcag gtcattcagg cttacatcga gagtggaata
    17701 cgtattagcg aacgcgtaca acggcaggtt ctcgttcatt ttgctaattt cttcgcacag
    17761 ctcgagatta tatttatccc agtcaaaaat aacgccgcac agacgcgcat tgttttcgat
    17821 cagttttaat aagtcgtcac ggtcgttcgg gtaaacaatc tggaagttca gacgttcaag
    17881 cgcgcgatga agttcacgga tgggttcttc tttaaaataa acccccatgt gattcaatat
    17941 tgcaataacg ttcatagtca tatctccagg taaaaaaggc ccctcccaac acatgggaca
    18001 aaatgaaagg aggagcctcg gaaaatactt ttaattaatg tgcgttagac gcggtgtggt
    18061 tatccattga gtggctctgg cgctcgtgca ttttgcgagc gtagaacatc aggataatca
    18121 ggctgacgat gaaggtacct gccagctcga aggagcttgc gcccatcagc gcgatgaagc
    18181 agaacacgca acccagtaca gagcagatca ggctgacaaa gttgcggatg ttaacgcctt
    18241 caaaacgaat caggtcaacg caagagtaga agtacggcag catagtcagc agtactgcga
    18301 taccggtcag ttcaccgaac aggtcagatg ctttaccacc ggcagagttc atcagagtga
    18361 tgaggatcat cagggcagtc attttcactg cagccagcag cagacctttt ttcggaatac
    18421 cgttgctgtc gacttcacca taaactttcg ggaagttacc gtcgttagcg gcacgtacac
    18481 ctgcctggcc taccaacatc atccaggagc ccagagaagt caggcacgca aaggcggtga
    18541 atgcagaaac cagcggcgca gcccagttac cgaggatagt tgaagcactg attgcaaacg
    18601 gagcaccgga agccgccatt acagaagacg gatacatacc ggaaagcacc tgagtcgcag
    18661 cgatataaac aatacctgct aaaccagtac ccagcatggt tgccagcgga acggtacgtt
    18721 tcgggttttt aaccatacca gtacttactg ctgcggattc aacacccacg aaggcccaca
    18781 ggcagagcag aatactttta atgatcgcat gaccatcagt ggtatccgca gtattccagt
    18841 tagctgcata agttgccgca tcaaaccaat gccagccaac aatagcagtc atcaccacag
    18901 gaataagaac cagcaccaga ccaatagtgg ttaaacggct tacccaggta ccgccgagca
    18961 tatttacaaa ggtaaatacc cagacgatag caatacaggc gatacccgcc ggaacaggat
    19021 catttaatac tgggaagaag gtggaaagat aagatacagc ggtaatacca atcgccaggt
    19081 taccaatcca gttagcatgg taataaagaa cacctgtctg aaaaccaaat gcaggggaaa
    19141 tttctccggc ataagcaatt gggccacctt gttgcgggtt ttttgttgcc agtcgggcat
    19201 atacatacgc cagcgacatt gcaccaataa tagagataat ccaaccccag atagcaatac
    19261 caccgatact tgctaggttc gcaggtaata atgcaatacc gctccccatc atattaccgg
    19321 caacaacacc ggtacaggca aatagcccga tcttcttggc agaactcatg ctcttctcct
    19381 aatttcattt ttgaatttgg agtccgggtc atgatgtata actatttcct gaccagacca
    19441 aactggcgat aagattactc acgaaaaaag gattaatcct aaagattagg tgaaataaca
    19501 caaaagtttc tgtaagtgag aacttgaggt tttttattaa cacatcagga tcgcaagttg
    19561 atatcatgaa aagataaaca tttaatgttt acaatggatt gcgtgacatt ctctggttaa
    19621 atttatgtaa taaaaattat gcggcaaata aattgccgca acatattata ccaacaggaa
    19681 catacaaaaa ctcaacaaca aatattcccg agcataaatc aacccgggag ttacttattc
    19741 tgaagcgaga aatttgtcga gataaggtac aacataagga acagaagtct ggaatatacc
    19801 attttcaatc cagtaaaggg tgtttgcccc tgggcgtaaa ttaaaggcgg tgaggtatgc
    19861 atcagctgct tcccggttca tccccttcat ttcataaacc ttgccaagca acacataatt
    19921 tagccaggac atttcaagat caatgccagt atttatcgcc tggtaagact catctgtttt
    19981 accttttacc agggcactga ccgcttttat ttgatatata atggacaggt tattcaattc
    20041 cggcagtgta acaatgttat ctatttctgt attcagtgct gctaattgtt tttcatctaa
    20101 aggatgttga gaatggcgca cgatatcaac taatgctttt tctgctctcg cgtaggtaaa
    20161 ttcgggggat gattgaacaa tctcacctag taattcactg gcacggttca atgatttatc
    20221 atcgccatgc agtaaataat catgtgcctg ataaaaatta gttaataacg caccacgatg
    20281 aggcaaaatt tgctggagca tctcctgcat tcgttgtggc cacggttggt ttaacgcttt
    20341 tgataaactc tccagtaaat cattttgaat cgccagctga tttccattag tgatgacata
    20401 acgtttatcc agcatggttg aaccatctgc attgtctacc aattttaccg acataaagca
    20461 ttgttgagca cggtattggc gctgattaac aaacgcaata gataatgttt taccggaact
    20521 gctcggttca tcaatgttgt agttgatttt gtcatgcacc ataaaggtgg agaaggtgtt
    20581 aagtgatgtc gccaccaaat cacccacgcc tatcgcgtaa gagagctgat acggggaact
    20641 ccagctgtta caacttttat ttaccatatt aatgtcaata tcgcgtggat tgagcaaaat
    20701 acgcgatttg ctcataggaa gacgtgtatc aagagttgaa aacgctacca gtgctacaca
    20761 gatacctaac gacaacagga aaaaaaacca tacccaaaag gtagtgaatc gtttgctttt
    20821 aactggagat tgttcaggtg gcgttgcggt gttttgaatg gtaagactgt gggagggaga
    20881 atctgtgtca ggaaccgcct ctggtatagg gggaggcgaa gatagcatta tttcctctcc
    20941 ctcttcttcg ctgtaccaga taaccggcac cattaattta tagccgcgct ttggtacagt
    21001 agcgatatag acaggactat cttcatcatt atcttttaat gatttacgta gttctgagat
    21061 actctgcgtc acaacgtgat tggtgacaat acttctcttc cagacattat cgataagttc
    21121 atccctgcta agtacttcgc cactgtgttg agcaaagaaa accagaagat cgattaatct
    21181 cggctcaagg gtaagttgac gcccattgcg gctaatttgg tttatggacg gagtaacaag
    21241 ccattcgcca acgcgaacta caggttgttg cataatagaa actcattcga aaagggaatg
    21301 gtacaatgat aattgccaca acctattttt accatctata gatggcttta tttacatatt
    21361 attggtgaat gcaagacgtt atttttacca gccataaact tcttgatcac atagtattac
    21421 gaaagaattt tactgagaac cagaagtaat attccttacc atcaaaattc atcatctttg
    21481 ccaaagaaaa atgtgcagaa aataatccat ggaaaattgc ccggagcgct tactatttta
    21541 atggattgtt agtctttgca tgagtaagcg gactgataca tttctctttg ttctcattca
    21601 aaaatctcat cagtcggcgt tctgcccgat gttgtgcttt atggatgcgt tacgctcctg
    21661 atgacgtcat ttgacgttca acagcatcac ggggccgcac gacatttcac gtcagtcagt
    21721 gctatagctc aggaacaaat tttcccgaat tgggatatac ccgcaaaatg ctggtgttgt
    21781 ggggagaatc tggttgagtt cggtagaaat gatttttagg cagaacgctt aaattgtggc
    21841 gtccagaaac gaaaaacgga cctccgtgga ggtccgttta tatgaatttg gtgcccggac
    21901 tcggaatgaa actggttata taaccaattg ttttgtatcg attgttttgt ctttgttgtt
    21961 ttcgaggcca ccaatgaggc ccccatgtgc aattcgaatt cttttgacgc tataatccct
    22021 cttttttgtg ctttgctgta tcattttgtc tggttcgata tgctgaattg cggaatcgaa
    22081 gtaacaacaa aatacagaga tttataaaat acctggctac ctgtgttagg gagcgttaac
    22141 gcacagattt tgtttatggc tgccagaaat gatctgatgg tttgtcgtct ggctctgcaa
    22201 gtaccctgtt gatatcccgg gaaccatgaa cgatacgttc gatacgaact tcagtatcca
    22261 gtaccctgaa cagcagaagg taacgaccat acgaacaacc tctgatccca gcccctaatt
    22321 cgggtctttc tctgtagagt actggcgatt cggctatcaa cagacattgt tggtaaagct
    22381 cttcggtaaa actgattgca cggacaggat tatcctgagc gatccagtca cctatagctt
    22441 caagatcgtg ttctgctaat ggtgaaacag tcagtttcat attggctgtt caccttctgc
    22501 catctttttg tatttgtgcg tcagtcgccc gaatacctct tcagcacttt tactttcacc
    22561 gctgttgatg cctgcggtaa cggcgctctg gagtgattca agctttatct gctgttcccg
    22621 ttcttccaga gcacgaagtc cggcacgaat gacttcactt gtattgttat accgaccact
    22681 ttctatttgt tcgcggataa acgtttcaaa gtaaggactg agggatacac tggtaggcat
    22741 ggtgattttc tcgccgattg attgctatca attaataata gttaatattc gcgaattgta
    22801 aaatacctct tttatgcctt gcggcttttg ttcactgtgc ttgccagggc ttccgggtag
    22861 tgtctggcaa taatatcatt aagcctgtca accagttctg gtttcctgaa cgtgatgtgt
    22921 actgagcctt tcttaaagta tttaatactg aacatctcgt cttcatagtc ctctttaccc
    22981 tgaacagact ggatatgatc atccagacga agggtgatgt tttcccggtt gtcggggatc
    23041 ggtttgcctc tgaacaggtg tagcattctt tccagatcgg caagtcgatc tgcctgttgc
    23101 ccggtgataa aatgaaaccc ccaccggtcc cacctcacca ggttgttgac gataatttta
    23161 ctgccaaatt tacagggtga gttggttttg taattccagc tcagtcccct gaagacgttg
    23221 atcacccctc gctcaaacac ctcatcctta ttctggtgca gttgttcaaa tgtgctcaga
    23281 atgtttgccg cactgatttc cggaaaatta tcgtattcca gtgaccggta ccatgtatca
    23341 cgtgcctgtg catccattaa tgacagcatc ccggaacgtt gcatcaggtc acgccagatt
    23401 tcgcggtcaa gattccgggt aatggctttc attgctgttt cgggtttctc catcagccag
    23461 caaccgcagc gaaaattctg tttcatggcc cagtccgggg cggttttacc tccaatactt
    23521 tctgtcattg ctgagatttc tgtcagttgc cggataagtg tgtcgatttg catcagtgtg
    23581 gtctttcgta cagccagaat gtgtccgatt gaggtggaac agataacgtc cgtatgcccg
    23641 ttgaccactt caggacggga gattgtgata cctgacataa tcgtttttcc tttttgcaga
    23701 taaatgaaaa cgccagccct gttcaggctg gcgtcagtgg aatgaagtat ggtgagtgat
    23761 atttaccggt tctggtgcag aagctgcaga tgacgaagag tacggacatc ctcccgggcc
    23821 tgttcgatac agggcaacag tgctgccgaa tccgttttat cctcatcgtt gtcaaagata
    23881 attcccgatt cgcagtcgat attgtcctgc agccacgcaa tgaggatatc cagcgctgtt
    23941 tcagtggtta atgatttcat gttgtttctg gttaccagtt gaaagtgggt aaacccggca
    24001 ggcattcagc gctggcatcc agatgaatga gactgacgcc ataacgccgg ataagtgtga
    24061 caaccagacg gcggaacatt tttgacagcc ccaggcgttt aagccgcaga accgggtatg
    24121 accacgcatc ggtacgtaac agatatccgc tacctgtaaa atgaatccat tctgattcgc
    24181 cgaagtcact ggtctggtgt gacagcgagt acaaccaggc gttgtctgtt tccgtgatat
    24241 gtgcggtgct gcagcgtatg ccggtaaggg ttgttaacgg tggtggtgtg caggttgact
    24301 gttggtcgga gtcgtccacc acatggagtg aataaccgtt ttcagcgact ttgttaatca
    24361 gttcagcgag ctcaatgcca tcgacgtcaa cgacaatgcg ccccatgttc agtgcctgca
    24421 cgttaatgct gtcggcttcc agtgtcaggg ccagtttcat tgttgcgcct ccggatgctt
    24481 accctgggta atgttattta ccattctgta attgtcgcgg gtcatcaggc ctgttgcccg
    24541 gcgagcccgg aggatatcga tgctgtttat taactgagag cgggtacagg cgctgaatcc
    24601 tggctggtca gttcgtacca gcgcgtattt ttccacgaga aagttcaccg catcacacag
    24661 tgaaatgcct gcctcaatat gctgctcaat cacacgttca tcggcaaacg gtgtgtcatt
    24721 cagtgtgagg ccataatgct ggttcagcag tcggatgagc agtgtctgcc agatggtgac
    24781 gggagacggg cagcgagatg cctcccgtac atgtgtgtcg gataatgttt tcatcttaag
    24841 ttctctgtaa ctggtagtgc taaaggtgag agcttcgggg ttatgaggtg gttgcgggtg
    24901 cctgtgccgg ataaacagcc agataaacgt aaccacagga gccgagggtg tcggcttcgc
    24961 aggtcagccc ttttgcatac agcgtgacgg tatgctgatt tcggggattc agttcaccgc
    25021 tggtgagcat gagctccagt tgtttcatca gcagcggaaa ggcctggtcc agatggtatg
    25081 catctgcgtc gctgaagcgg cctctgatac cggcgcggtc ggcaaggtaa tgcaggcggt
    25141 taccctcctg caccagacgg gcaccaaaac agggcctcac tgtgcaggga agcccccacc
    25201 aggggcggtc gtgattgtcg tcgggatgag ttatcccgga gagtttgtct gacacgataa
    25261 aatcctctct gaaaagtggt taagaatggt ttaacgatga caggtcagcc ggcgcgctga
    25321 ccggtttcgg tgaagatgta tgcattgacg agaatggcct catccacagc ggcgtctgag
    25381 tccatctggt cgtactcgtt ttccgggtga cggaacatca gttcagggtg acgctcatca
    25441 gccggacata cgggccaaaa ctgtccttac ggcgttcggc aaacacggcc agcacaccgg
    25501 gaatatcctg tacttcacga ccggtatacg cttcagtgct gccttgccag cggtatttgc
    25561 cggtgcagaa cggaaaaaga cgggatgcag gatgctgccg gtgaatgcgc atggcttcac
    25621 cacgggtgat aattttcata atgggatacc tctgaagaca gaagataaaa gtgaaaacag
    25681 gtgtgatgtg gttgtgacgg gttaaagcag accatgttcg gcaaaggaga aaacctggct
    25741 gccaccgact atcagatggt ccggcacccg gatatccacc aggcccagtg cctgtaccag
    25801 acgttccgtg ataaggcggt ctgccttact gggggtgact tcaccggacg ggtgattgtg
    25861 tgccagcacc acggctgcgg cattgtggta cagggcgcgt ttaatcactt cccggggatg
    25921 gacttccgtg cggttgatgg tgccggtaaa gagggtttca ccggcaatca gctgattctg
    25981 gttgttcaga tacagcaccc ggaattcttc acgctccagt cccgccatgt tcagaatcag
    26041 ccattcccgt gccgctcggg tggaggtgaa ggccacgccg ggttcatgaa gatggcggtc
    26101 cagggttttc agggcccgca gaatgagact gcgctcgccg ggcgtcatct ctccgggcag
    26161 aaaggaaagc tgctgcattg tccctgcctc cattcagtcg atgatgcgca taatggcgct
    26221 gcattccgga tgctgcaggg cgtaatcccg cagccggtaa taatggaccg tcatggcata
    26281 acactccgta cgacaggcgt gatggctgta cgccatcaga caggcggcaa taccggcagc
    26341 ttccgggctc atttcagcgc ggttaccgtt catggcattg aacagtaccc atgtttctcc
    26401 atcatcatca tccggttcgg gggccataaa tgccccgccg ttgttcaggg tgtacagatt
    26461 ccagatacca tcgcagtagt tttcgcaaag acggtccatc cagccgaaga tacggggctc
    26521 cagggtgacc cactgtggaa tgaggccaaa gtgctgcggc cagaagctga tgcgctgttc
    26581 atcagggact atggtggcaa ccaactgaga ctggttattc cctgatgcag tggttacgga
    26641 aacagaagat gtggtgaaat tatgcgagac ggttgtcatg agattattcc ttataaaaag
    26701 taaatgaatg gaagaagtcg cgggccggga cggacatgag tcagaactgc gctttcaggg
    26761 aaaagg
//
LOCUS       DAZGSP010000070        26273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZGSP010000070 DAZGSP010000000
VERSION     DAZGSP010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.070"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHK74_003204"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHK74_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP0221970.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            414..608
                     /gene="bfd"
                     /locus_tag="ACHK74_003205"
     CDS             414..608
                     /gene="bfd"
                     /locus_tag="ACHK74_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFP0221971.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            681..1157
                     /gene="bfr"
                     /locus_tag="ACHK74_003206"
     CDS             681..1157
                     /gene="bfr"
                     /locus_tag="ACHK74_003206"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFP0221972.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1154..1621)
                     /locus_tag="ACHK74_003207"
     CDS             complement(1154..1621)
                     /locus_tag="ACHK74_003207"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP0221973.1"
                     /translation="MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            2000..2311
                     /gene="rpsJ"
                     /locus_tag="ACHK74_003208"
     CDS             2000..2311
                     /gene="rpsJ"
                     /locus_tag="ACHK74_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFP0221974.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2344..2973
                     /gene="rplC"
                     /locus_tag="ACHK74_003209"
     CDS             2344..2973
                     /gene="rplC"
                     /locus_tag="ACHK74_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFP0221975.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2984..3589
                     /gene="rplD"
                     /locus_tag="ACHK74_003210"
     CDS             2984..3589
                     /gene="rplD"
                     /locus_tag="ACHK74_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFP0221976.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3586..3888
                     /gene="rplW"
                     /locus_tag="ACHK74_003211"
     CDS             3586..3888
                     /gene="rplW"
                     /locus_tag="ACHK74_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFP0221977.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3906..4727
                     /gene="rplB"
                     /locus_tag="ACHK74_003212"
     CDS             3906..4727
                     /gene="rplB"
                     /locus_tag="ACHK74_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFP0221978.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4744..5022
                     /gene="rpsS"
                     /locus_tag="ACHK74_003213"
     CDS             4744..5022
                     /gene="rpsS"
                     /locus_tag="ACHK74_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFP0221979.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5037..5369
                     /gene="rplV"
                     /locus_tag="ACHK74_003214"
     CDS             5037..5369
                     /gene="rplV"
                     /locus_tag="ACHK74_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFP0221980.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5387..6088
                     /gene="rpsC"
                     /locus_tag="ACHK74_003215"
     CDS             5387..6088
                     /gene="rpsC"
                     /locus_tag="ACHK74_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFP0221981.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6101..6511
                     /gene="rplP"
                     /locus_tag="ACHK74_003216"
     CDS             6101..6511
                     /gene="rplP"
                     /locus_tag="ACHK74_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFP0221982.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6511..6702
                     /gene="rpmC"
                     /locus_tag="ACHK74_003217"
     CDS             6511..6702
                     /gene="rpmC"
                     /locus_tag="ACHK74_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFP0221983.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6702..6956
                     /gene="rpsQ"
                     /locus_tag="ACHK74_003218"
     CDS             6702..6956
                     /gene="rpsQ"
                     /locus_tag="ACHK74_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFP0221984.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7121..7492
                     /gene="rplN"
                     /locus_tag="ACHK74_003219"
     CDS             7121..7492
                     /gene="rplN"
                     /locus_tag="ACHK74_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFP0221985.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7503..7817
                     /gene="rplX"
                     /locus_tag="ACHK74_003220"
     CDS             7503..7817
                     /gene="rplX"
                     /locus_tag="ACHK74_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFP0221986.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7832..8371
                     /gene="rplE"
                     /locus_tag="ACHK74_003221"
     CDS             7832..8371
                     /gene="rplE"
                     /locus_tag="ACHK74_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFP0221987.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8386..8691
                     /gene="rpsN"
                     /locus_tag="ACHK74_003222"
     CDS             8386..8691
                     /gene="rpsN"
                     /locus_tag="ACHK74_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP0221988.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8725..9117
                     /gene="rpsH"
                     /locus_tag="ACHK74_003223"
     CDS             8725..9117
                     /gene="rpsH"
                     /locus_tag="ACHK74_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFP0221989.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9130..9663
                     /gene="rplF"
                     /locus_tag="ACHK74_003224"
     CDS             9130..9663
                     /gene="rplF"
                     /locus_tag="ACHK74_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFP0221990.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9673..10026
                     /gene="rplR"
                     /locus_tag="ACHK74_003225"
     CDS             9673..10026
                     /gene="rplR"
                     /locus_tag="ACHK74_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFP0221991.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10041..10544
                     /gene="rpsE"
                     /locus_tag="ACHK74_003226"
     CDS             10041..10544
                     /gene="rpsE"
                     /locus_tag="ACHK74_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFP0221992.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10548..10727
                     /gene="rpmD"
                     /locus_tag="ACHK74_003227"
     CDS             10548..10727
                     /gene="rpmD"
                     /locus_tag="ACHK74_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFP0221993.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10731..11165
                     /gene="rplO"
                     /locus_tag="ACHK74_003228"
     CDS             10731..11165
                     /gene="rplO"
                     /locus_tag="ACHK74_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFP0221994.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11173..12504
                     /gene="secY"
                     /locus_tag="ACHK74_003229"
     CDS             11173..12504
                     /gene="secY"
                     /locus_tag="ACHK74_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFP0221995.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12536..12652
                     /gene="rpmJ"
                     /locus_tag="ACHK74_003230"
     CDS             12536..12652
                     /gene="rpmJ"
                     /locus_tag="ACHK74_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFP0221996.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12799..13155
                     /gene="rpsM"
                     /locus_tag="ACHK74_003231"
     CDS             12799..13155
                     /gene="rpsM"
                     /locus_tag="ACHK74_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFP0221997.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13172..13561
                     /gene="rpsK"
                     /locus_tag="ACHK74_003232"
     CDS             13172..13561
                     /gene="rpsK"
                     /locus_tag="ACHK74_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFP0221998.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13595..14215
                     /gene="rpsD"
                     /locus_tag="ACHK74_003233"
     CDS             13595..14215
                     /gene="rpsD"
                     /locus_tag="ACHK74_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFP0221999.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14241..15230
                     /gene="rpoA"
                     /locus_tag="ACHK74_003234"
     CDS             14241..15230
                     /gene="rpoA"
                     /locus_tag="ACHK74_003234"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFP0222000.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15271..15654
                     /gene="rplQ"
                     /locus_tag="ACHK74_003235"
     CDS             15271..15654
                     /gene="rplQ"
                     /locus_tag="ACHK74_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFP0222001.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15761..16129
                     /locus_tag="ACHK74_003236"
     CDS             15761..16129
                     /locus_tag="ACHK74_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFP0222002.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGGGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            16140..16565
                     /gene="zntR"
                     /locus_tag="ACHK74_003237"
     CDS             16140..16565
                     /gene="zntR"
                     /locus_tag="ACHK74_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFP0222003.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16621..16839
                     /gene="arfA"
                     /locus_tag="ACHK74_003238"
     CDS             16621..16839
                     /gene="arfA"
                     /locus_tag="ACHK74_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFP0222004.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16836..17246)
                     /gene="mscL"
                     /locus_tag="ACHK74_003239"
     CDS             complement(16836..17246)
                     /gene="mscL"
                     /locus_tag="ACHK74_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFP0222005.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17376..18752)
                     /gene="trkA"
                     /locus_tag="ACHK74_003240"
     CDS             complement(17376..18752)
                     /gene="trkA"
                     /locus_tag="ACHK74_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFP0222006.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18774..20063)
                     /gene="rsmB"
                     /locus_tag="ACHK74_003241"
     CDS             complement(18774..20063)
                     /gene="rsmB"
                     /locus_tag="ACHK74_003241"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFP0222007.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20109..21056)
                     /gene="fmt"
                     /locus_tag="ACHK74_003242"
     CDS             complement(20109..21056)
                     /gene="fmt"
                     /locus_tag="ACHK74_003242"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFP0222008.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21071..21580)
                     /gene="def"
                     /locus_tag="ACHK74_003243"
     CDS             complement(21071..21580)
                     /gene="def"
                     /locus_tag="ACHK74_003243"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP0222009.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21710..22834
                     /gene="dprA"
                     /locus_tag="ACHK74_003244"
     CDS             21710..22834
                     /gene="dprA"
                     /locus_tag="ACHK74_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFP0222010.1"
                     /translation="MVDTEMWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAIVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22806..23279
                     /gene="smg"
                     /locus_tag="ACHK74_003245"
     CDS             22806..23279
                     /gene="smg"
                     /locus_tag="ACHK74_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFP0222011.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23308..23850
                     /locus_tag="ACHK74_003246"
     CDS             23308..23850
                     /locus_tag="ACHK74_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFP0222012.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23855..24427
                     /gene="tsaC"
                     /locus_tag="ACHK74_003247"
     CDS             23855..24427
                     /gene="tsaC"
                     /locus_tag="ACHK74_003247"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFP0222013.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24432..25250
                     /gene="aroE"
                     /locus_tag="ACHK74_003248"
     CDS             24432..25250
                     /gene="aroE"
                     /locus_tag="ACHK74_003248"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFP0222014.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25247..25504
                     /locus_tag="ACHK74_003249"
     CDS             25247..25504
                     /locus_tag="ACHK74_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFP0222015.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25480..26034)
                     /locus_tag="ACHK74_003250"
     CDS             complement(25480..26034)
                     /locus_tag="ACHK74_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFP0222016.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6732 a         6247 c         6817 g         6477 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccacagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gttgctaaag ttctgggcta attacacgtt
      241 aattagtttt gaattgaaaa gggcgcttcg gcgccctttt tgctgcccgt aatttgagct
      301 cgaaatattt agtcgtaatt ttgctcgctg aactatggcg caaataaaac tgtgctattg
      361 taatcataac cattctcatt tacactttgc gcagaatttg aacgggagca atcatgtacg
      421 tttgtttgtg taatggtgta agtgacaaaa aaattcgcca ggcagtccgt cagtttcatc
      481 cgcaatcttt ccaacaattg cgtaagttta ttcctgtggg aaatcaatgc ggtaagtgca
      541 ttcgcgccgc gcgcgaagtg atgcaggacg agttgatgca gatgccagaa tttaaagaga
      601 ttgcctgaag accacgctta ttttttgaca tcccgctagc cccatctacg ctctaatagt
      661 ggaagcggag ggactataaa atgaaaggtg atgttaaaat cataaattat ctcaataaac
      721 tattgggaaa tgagcttgtc gcaatcaatc agtattttct ccatgcccgg atgtttaaaa
      781 actggggcct gatgcgtctc aatgacgtcg aataccatga atccatcgat gagatgaaac
      841 acgctgataa atatatcgag cgtattttgt ttttagaagg aatccccaac ttacaggatt
      901 taggcaaact ggggattggt gaagatgtcg aggagatgct gcaatccgac ctgcgattag
      961 aactggaagg cgcgaaggat ctccgtgagg ctatcgcgta tgccgacagc gttcatgact
     1021 atgttagccg tgatatgatg atcgagatcc ttgccgatga agaaggtcat attgactggc
     1081 tcgaaactga gctggatctc atcggtaaaa tcggtttaca aaattacctg caatcacaaa
     1141 taaaagttaa agattaacgg cctgccagca aactatccca acctacaacg aaacccgcag
     1201 ccgccagaaa tggcccaaac ggcagcgggt tttttaatga ctcttttcct ctcatgagca
     1261 gaccaacaac tacggctccg caagcgaatg aggcagcaag gaaaaccagt cgtggcaaga
     1321 aggtccaggt atgccaggca ccaagggcgg cgagaaactt cacatcacca tagcctaatc
     1381 cttctttatg acgcagtatg cgataccccc agtaaatgac agcgaatgtg ccgtaaccaa
     1441 taatcgcccc ccataacgca tcggctaaac cgtcaggatt acaaacctga gagaacaaaa
     1501 gacctgacca gagtaacggg caggtaaatc tgtcaggtaa caagccatgc tttgcatccc
     1561 ataaaaaaag taggacagat aaacaagcgt acaaaatcag aaagggaagg gtagcgtcca
     1621 taaaacatcc tctgtaattg gagtatcacc atcgaataac tccggatgct cagcaacaga
     1681 gaaatcgctg atttgttaac ctggttccgg tgaaaaacgc tatcttcaca accttacaaa
     1741 tttcgtggaa tacagctcgc aatctgcgcg ttgcgattaa tttctacccg atgcttgcgt
     1801 caggctcaga tttgcgtata atgcgcgggc ttgtcgtagt tgacagcagg ttcaatctga
     1861 acctcagtta gccgaatttg gctacctaac aatgctccca atcggggagc tacgtaagaa
     1921 cggttacact ctcccatcaa tcgtaatggg tctgaggagt aatcattttc gtttataaaa
     1981 taattggagc tctggtctca tgcagaacca aagaatccgt atccgcctga aagcgtttga
     2041 tcatcgtctg atcgatcaag caaccgcgga aatcgtcgag actgccaagc gcactggtgc
     2101 gcaggtccgt ggtccgatcc cgctgccgac acgcaaagag cgcttcactg ttctgatctc
     2161 cccgcacgtc aacaaagacg cgcgcgatca gtacgaaatc cgtactcact tgcgtctggt
     2221 tgacatcgtt gagccaaccg agaaaaccgt tgatgctctg atgcgtctgg atctggctgc
     2281 cggtgtagac gtgcagatca gcctgggtta atcaggtcat tgagcgattg agaggttgaa
     2341 acaatgattg gtttagtcgg taaaaaagtg ggtatgaccc gtatcttcac agaagacggc
     2401 gtttctatcc cagtaaccgt aatcgaagtt gaagcaaacc gcgttactca ggttaaagac
     2461 ctggctaacg atggctaccg tgctattcag gtgaccaccg gtgctaaaaa agctaaccgt
     2521 gtgaccaagc ctgaagctgg ccacttcgct aaagctggcg tagaagctgg ccgtggtctg
     2581 tgggaattcc gcctggctga aggcgaagag ttcactgtag gtcagagcat tagcgttgaa
     2641 ctgtttgctg acgttaaaaa agttgacgta actggcacct ctaaaggtaa aggtttcgca
     2701 ggtaccgtta agcgctggaa cttccgtacc caggacgcta ctcacggtaa ctccttgtct
     2761 caccgcgttc cgggttctat cggtcagaac cagactccgg gcaaagtgtt caaaggcaag
     2821 aaaatggcag gtcagatggg taacgaacgt gtaaccgttc agagccttga cgtagtacgc
     2881 gttgacgctg agcgcaacct gctgctggtt aaaggtgctg tcccgggtgc aaccggtagc
     2941 gacctgatcg ttaaaccagc tgtgaaggcg taaggagata gcaatggaat tagtattgaa
     3001 agacgcgcag agcgcgctga ctgtttccga aactaccttc ggtcgtgatt tcaacgaagc
     3061 gctggttcac caggttgttg ttgcttatgc agctggtgct cgtcagggta ctcgtgctca
     3121 gaagactcgt gctgaagtaa ctggttccgg taaaaaaccg tggcgccaga aaggcaccgg
     3181 ccgtgcgcgt tctggttcta tcaagagccc gatctggcgt tctggtggcg tgacctttgc
     3241 tgctcgtccg caggaccaca gtcaaaaagt taacaagaag atgtaccgcg gcgcgctgaa
     3301 aagcatcctg tccgaactgg tacgtcagga tcgtctgatc gttgtcgaga agttctctgt
     3361 agaagcgccg aaaactaagc tgctggcaca gaaactgaaa gacatggctc tggaagatgt
     3421 gctgatcatc accggtgagc tggacgaaaa cctgttcctg gctgcgcgca acctgcacaa
     3481 ggttgacgta cgcgatgcaa ctggtatcga cccggttagc ctgatcgcct tcgacaaagt
     3541 cgtaatgact gctgatgctg ttaagcaagt tgaggagatg ctggcatgat tcgtgaagaa
     3601 cgtctgctga aggtgctgcg tgcaccgcac gtttctgaaa aagcgtctac tgcgatggaa
     3661 aaatccaaca ccatcgtact caaagttgct aaagacgcga ccaaagcaga aatcaaagct
     3721 gctgtgcaga aactgtttga agtcgaagtc gaagtcgtta acaccctggt agttaaaggg
     3781 aaagttaaac gtcacggaca gcgtatcggt cgtcgtagcg actggaaaaa agcttacgtc
     3841 accctgaaag aaggccagaa tctggacttc gttggcggcg ctgagtaagt cggaggagta
     3901 atacaatggc agttgttaaa tgtaaaccga catctccggg tcgtcgccac gtagttaaag
     3961 tggttaaccc tgagctgcac aagggcaaac cttttgctcc gttgctggaa aaaaacagca
     4021 aatccggtgg tcgtaacaac aatggccgta tcaccactcg tcatatcggt ggtggccaca
     4081 agcaggctta ccgtattgtt gacttcaaac gcaacaaaga cggtatcccg gcagttgttg
     4141 aacgtcttga gtacgatccg aaccgttccg cgaacatcgc gctggttctg tacaaagacg
     4201 gtgaacgccg ttacatcctg gcccctaaag gcctgaaagc tggcgaccag attcagtctg
     4261 gcgttgatgc tgcaatcaaa ccaggtaaca ccctgccgat gcgcaacatc ccggttggtt
     4321 ctactgttca taacgtagaa atgaaaccag gtaaaggcgg tcagctggca cgttccgctg
     4381 gtacttacgt tcagatcgtt gctcgtgatg gtgcttatgt caccctgcgt ctgcgttctg
     4441 gtgaaatgcg taaagtagaa gcagactgcc gtgcaactct gggcgaagtt ggcaatgctg
     4501 agcatatgct gcgcgttctg ggtaaagcag gtgctgcacg ctggcgtggt gttcgtccga
     4561 ccgttcgcgg taccgcgatg aacccggtag accacccaca tggtggtggt gaaggtcgta
     4621 actttggtaa gcacccggta actccgtggg gcgttcagac caaaggtaag aagacccgca
     4681 gcaacaagcg tactgataaa ttcatcgtac gtcgccgtag caaataattt tagaggataa
     4741 gccatgccac gttctctcaa gaaaggtcct tttattgacc tgcacttgct gaagaaggta
     4801 gagaaagcgg tggaaagcgg agacaagaag cccctgcgca cttggtcccg tcgttcaacg
     4861 atctttccta acatgatcgg tttgaccatc gctgtccata atggtcgtca gcacgttccg
     4921 gtatttgtaa ccgacgaaat ggtcggtcac aaactgggtg aattcgcacc gactcgtact
     4981 tatcgcggcc acgctgctga taaaaaagcg aagaagaaat aaggtaggag gaagagatgg
     5041 aaactatcgc taaacatcgc catgctcgtt cttctgctca gaaggttcgc cttgttgctg
     5101 acctgattcg cggtaagaaa gtgtcgcagg ctctggatat tttgacctac accaacaaga
     5161 aagcggctgt actggtcaag aaagttctgg aatctgccat tgctaacgct gaacacaacg
     5221 atggcgctga cattgacgat ctgaaagtta cgaaaatttt cgtagacgaa ggcccgagca
     5281 tgaagcgcat tatgccgcgt gcaaaaggtc gtgcagatcg catcctgaag cgcaccagcc
     5341 acatcactgt ggttgtgtcc gatcgctgag actctggaga ctagcaatgg gtcagaaagt
     5401 acatcctaat ggtattcgcc tgggtattgt aaaaccatgg aactctacct ggtttgcgaa
     5461 caccaaagaa ttcgctgaca acctggacag cgattttaaa gtacgtcagt acctgactaa
     5521 ggaactggct aaagcgtccg tatctcgtat cgttatcgag cgtccggcta agagcatccg
     5581 tgtaaccatt cacactgctc gcccgggtat cgttatcggt aaaaaaggtg aagacgtaga
     5641 aaaactgcgt aaggtcgtag cggacatcgc tggcgttcct gcacagatca acatcgccga
     5701 agttcgtaag cctgaactgg acgcaaaact ggttgctgac agcatcactt ctcagctgga
     5761 acgtcgcgtt atgttccgtc gtgctatgaa gcgtgctgta cagaacgcaa tgcgtctggg
     5821 cgctaaaggt attaaagttg aagttagcgg ccgtctgggc ggcgcggaaa tcgcacgtac
     5881 cgaatggtac cgcgaaggtc gcgtaccgct gcacactctg cgtgctgaca tcgactacaa
     5941 cacctctgaa gcgcacacca cttacggtgt aatcggcgtt aaagtgtgga tcttcaaagg
     6001 cgagatcctg ggtggtatgg ctgctgttga acaaccggaa aaaccggctg ctcagcctaa
     6061 aaagcagcag cgtaaaggcc gtaaataagg agcgtcgctg atgttacaac caaagcgtac
     6121 aaaattccgt aaaatgcaca aaggccgtaa ccgcggtctg gcgcagggta cggatgttag
     6181 cttcggcagc ttcggtctga aagctgttgg ccgtggtcgt ctgactgccc gtcagatcga
     6241 agcagcacgt cgtgctatga cccgtgcagt taagcgtcaa ggtaagatct ggatccgtgt
     6301 gttcccggac aaaccgatca ctgaaaagcc gctggcagtg cgtatgggta aaggtaaagg
     6361 taacgtggag tattgggttg ccttgattca gccgggtaaa gtcctgtatg aaatggacgg
     6421 tgttccggaa gagctggccc gtgaagcatt caagctggca gcagcgaaac tgccgattaa
     6481 aaccaccttt gtaactaaga cggtgatgta atgaaagcaa aagagctgcg tgagaagagc
     6541 gttgaagagc tgaacaccga gctgctgaac ctgctgcgtg agcagttcaa cctgcgtatg
     6601 caggctgcaa gtggccagct gcaacagtct cacctgttga agcaagtgcg tcgcgatgtc
     6661 gcacgcgtta agactttact gaacgagaag gcgggtgcgt aatgaccgat aaaatccgta
     6721 ctctgcaagg tcgcgttgtt agcgacaaaa tggagaaatc cattgttgtt gctatcgaac
     6781 gttttgtgaa acacccgatc tacggtaaat tcatcaagcg tacgaccaaa ctgcacgtac
     6841 atgacgagaa caacgaatgc ggtatcggtg acgtggttga aatccgcgaa tgccgtccgc
     6901 tgtccaagac taaatcctgg acgctggttc gcgttgtaga gaaagcggtt ctgtaataca
     6961 gtacactctc tcgatacgaa taaacggctc agaaatgagc cgtttatttt ttctacccat
     7021 atccttgaag cggtgttata atgccgcgcc ctcgatatgg ggatttttaa cgacctgatt
     7081 ttcgggtctc agtagtagtt gacattagcg gagcactaaa atgatccaag aacagactat
     7141 gctgaacgtc gccgacaact ccggtgcacg tcgcgtaatg tgtatcaagg ttctgggtgg
     7201 ctcgcaccgt cgctacgcag gcgtaggcga catcatcaag atcaccatca aagaagcaat
     7261 tccgcgtggt aaggtcaaaa aaggtgatgt gctgaaggcg gtagtggtgc gcaccaagaa
     7321 gggtgttcgt cgcccggacg gttctgtcat tcgcttcgat ggtaatgctt gtgttcttct
     7381 gaacaacaac agcgagcagc ctatcggtac gcgtattttt gggccggtaa ctcgtgagct
     7441 tcgtagtgag aagttcatga aaattatctc tctggcacca gaagtactct aaggagcgaa
     7501 tcatggcagc gaaaatccgt cgtgatgacg aagttatcgt gttaaccggt aaagataaag
     7561 gtaaacgcgg taaagttaag aatgtcctgt cttccggcaa ggtcattgtt gaaggtatca
     7621 acctggttaa gaaacatcag aagccggttc cggccctgaa ccaaccgggt ggcatcgttg
     7681 aaaaagaagc cgctattcag gtttccaacg tagcaatctt caatgcggca accggcaagg
     7741 ctgaccgtgt aggctttaga ttcgaagacg gcaaaaaagt ccgtttcttc aagtctaaca
     7801 gcgaaactat caagtaattt ggagtagtac gatggcgaaa ctgcatgatt actacaaaga
     7861 cgaagtagtt aaaaaactca tgactgagtt taactacaat tctgtcatgc aagtccctcg
     7921 ggtcgagaag atcaccctga acatgggtgt tggtgaagcg atcgctgaca aaaaactgct
     7981 ggataacgca gcagcagacc tggcagcaat ctccggtcaa aaaccgctga tcaccaaagc
     8041 acgcaaatct gttgcaggct tcaaaatccg tcagggctat ccgatcggct gtaaagtaac
     8101 tctgcgtggc gaacgcatgt gggagttctt tgagcgcctg atcactattg ctgtacctcg
     8161 tatccgtgac ttccgtggcc tgtccgctaa gtctttcgac ggtcgtggta actacagcat
     8221 gggtgtccgt gagcagatca tcttcccaga aatcgactac gataaagtcg accgcgttcg
     8281 tggtttggat attaccatta ccactactgc gaaatctgac gaagaaggcc gcgctctgct
     8341 ggctgccttt gacttcccgt tccgcaagta aggtagggtt actaaatggc taagcaatca
     8401 atgaaagcac gcgaagtaaa acgcgtagct ttagctgata aatacttcgc gaaacgcgct
     8461 gaactgaaag cgatcatctc tgatgtgaac gcttccgacg aagatcgttg gaacgctgtt
     8521 ctcaagctgc agactctgcc gcgtgattcc agcccgtctc gtcagcgtaa ccgctgccgt
     8581 caaacaggtc gtccgcatgg tttcctgcgg aagttcgggt tgagccgtat taaggtccgt
     8641 gaagccgcta tgcgcggtga aatcccgggt ctgaaaaagg ctagctggta attgtcacca
     8701 attgaatcac gggagtaaag acagatgagc atgcaagatc cgatcgcgga tatgctgacc
     8761 cgtatccgta acggtcaggc cgcgaacaaa gctgcggtca ccatgccttc ctccaagctg
     8821 aaagtggcaa tcgccaacgt gctgaaggaa gaaggtttta ttgaagattt taaagttgaa
     8881 ggcgacacca agcctgaact ggaacttact ctgaagtatt tccagggcaa agctgttgta
     8941 gaaagcattc agcgtgtcag ccgcccaggt ctgcgcatct ataaacgtaa agatgagctg
     9001 ccgaaagtta tggcgggtct gggtatcgca gttgtttcta cctctaaagg tgttatgact
     9061 gatcgtgcag cgcgccaggc tggtcttggt ggcgaaatta tctgctacgt agcctaatcg
     9121 gaggaaaaaa tgtctcgtgt tgctaaagca ccggtcgttg ttcctgccgg cgttgacgta
     9181 aaaatcaacg gtcaggttat tacgatcaaa ggtaaaaacg gcgagctgac tcgtactctc
     9241 aacgatgctg ttgaagttaa acatgcagat aataccctga ccttcggtcc gcgtgatggt
     9301 tacgcagacg gttgggcaca ggctggtacc gcgcgtgccc tgctgaactc aatggttatc
     9361 ggtgttaccg aaggcttcac taagaagctg cagctggttg gtgtaggtta ccgtgcagcg
     9421 gttaaaggta atgtgattaa cctgtctctg ggtttctctc atcctgttga ccatcagctg
     9481 cctgcgggta tcactgctga atgtccgact cagactgaaa tcgtgctgaa aggcgctgat
     9541 aagcaggtga tcggccaggt tgcagcggat ctgcgcgcct accgtcgtcc tgagccttat
     9601 aaaggcaagg gtgttcgtta cgccgacgaa gtcgtgcgta ccaaagaggc taagaagaag
     9661 taaggtaaca ctatggataa gaaatctgct cgtatccgtc gtgcgacccg cgcacgccgc
     9721 aagctccagg agctgggcgc aactcgcctg gtggtacatc gtaccccgcg tcacatttac
     9781 gcacaggtaa ttgcaccgaa cggttctgaa gttctggtag ctgcttctac tgtagaaaaa
     9841 gctatcgctg aacaactgaa gtacaccggt aacaaagacg cggctgcagc tgtgggtaaa
     9901 gctgtcgctg aacgcgctct ggaaaaaggc atcaaagatg tatcctttga ccgttccggg
     9961 ttccaatatc atggtcgtgt ccaggcactg gcagatgctg cccgtgaagc tggccttcag
    10021 ttctaaggta gaggtgtaag atggctcaca tcgaaaaaca agctggcgaa ctgcaggaaa
    10081 agctgatcgc ggtaaaccgc gtatctaaaa ccgttaaagg tggtcgtatt ttctccttca
    10141 cagctctgac tgtagttggc gatggtaacg gtcgcgttgg ttttggttac ggtaaagcgc
    10201 gtgaagttcc agcagcgatc cagaaagcga tggaaaaagc ccgtcgcaat atgattaacg
    10261 tcgcgctgaa taacggcact ctgcaacacc ctgttaaagg tgttcacacg ggttctcgcg
    10321 tattcatgca gccggcttcc gaaggtaccg gtatcatcgc cggtggtgca atgcgcgccg
    10381 ttctggaagt cgctggggtt cataacgttc tggctaaagc atatggttcc accaacccga
    10441 tcaacgtggt tcgtgcaact attgatggcc tggaaaatat gaattctcca gaaatggtcg
    10501 ctgccaagcg tggtaaatcc gttgaagaaa ttctggggaa ataaaccatg gcaaagacta
    10561 ttaaaattac tcaaacccgc agtgcaatcg gtcgtctgcc gaaacacaag gcaacgctgc
    10621 ttggcctggg tctgcgtcgt attggtcaca ccgtagagcg cgaggatact cctgctattc
    10681 gcggtatgat caacgcggtt tccttcatgg ttaaagttga ggagtaagag atgcgtttaa
    10741 atactctgtc tccggccgaa ggctccaaaa aggcgggtaa acgcctgggt cgtggtatcg
    10801 gttctggcct cggtaaaacc ggtggtcgtg gtcacaaagg tcagaagtct cgttctggcg
    10861 gtggcgtacg tcgcggtttc gagggtggtc agatgcctct gtaccgtcgt ctgccgaaat
    10921 tcggcttcac ttctcgtaaa gcagcgatta cagccgaagt tcgtctgtct gacctggcta
    10981 aagtagaagg cggtgttgta gacctgaaca cgctgaaagc ggctaacatt atcggtatcc
    11041 agatcgagtt cgcgaaagtg atcctggctg gcgaagtaac gactccggta actgttcgtg
    11101 gcctgcgtgt tactaaaggc gctcgtgctg ctatcgaagc tgctggcggt aaaatcgagg
    11161 aataagtagc agatggctaa acaaccggga ttagattttc aaagtgccaa aggtggctta
    11221 ggcgagctga aacgcagact gctgtttgtt atcggtgcgc tgattgtgtt ccgtattggc
    11281 tcttttattc cgatccctgg tattgatgcc gctgtacttg ccaaactgct tgagcaacag
    11341 cgaggcacca tcattgagat gtttaacatg ttctctggtg gtgctctcag ccgtgcttct
    11401 atctttgctc tggggatcat gccgtatatt tcggcgtcga tcattatcca gctgctgacg
    11461 gtggttcacc caacgttggc agaaattaag aaagaagggg agtctggtcg tcgtaagatc
    11521 agccagtaca cccgttacgg tactctggtg ctggcaatat tccagtcgat cggtattgct
    11581 accggtctgc cgaatatgcc tggtatgcaa ggcctggtga ttaacccagg ctttgcattc
    11641 tacttcaccg ctgttgtaag tctggtcaca ggaacgatgt tcctgatgtg gttgggcgaa
    11701 cagattactg aacgaggtat cggcaacggt atttcaatca ttatcttcgc cggtattgtc
    11761 gcgggactcc cgccagccat tgcccatact atcgagcaag cgcgtcaagg cgacctgcac
    11821 ttcctcgtgt tgctgttggt tgcagtatta gtatttgcag tgacgttctt tgttgtattt
    11881 gttgagcgtg gtcaacgccg cattgtggta aactacgcga aacgtcagca aggtcgtcgt
    11941 gtctatgctg cacagagcac acatttaccg ctgaaagtga atatggcggg ggtaatcccg
    12001 gcaatcttcg cttccagtat tattctgttc ccggcgacca tcgcgtcatg gttcgggggc
    12061 ggtactggtt ggaactggct gacaacaatt tcgctgtatt tgcagcctgg gcaaccgctt
    12121 tatgtgttac tctatgcgtc tgcaatcatc ttcttctgtt tcttctacac ggcgttggtt
    12181 ttcaacccgc gtgaaacagc agataacctg aagaagtccg gtgcatttgt accaggaatt
    12241 cgtccgggag agcaaacggc gaagtatatc gataaagtaa tgacccgcct gaccctggtt
    12301 ggtgcgctgt atattacctt tatctgcctg atcccggagt tcatgcgtga tgcaatgaaa
    12361 gtaccgttct acttcggtgg gacctcactg cttatcgttg ttgtcgtgat tatggacttt
    12421 atggctcaag tgcaaactct gatgatgtcc agtcagtatg agtctgcatt gaagaaggcg
    12481 aacctgaaag gctacggccg ataattggtc gcccgagaag ttacggagag taaaaatgaa
    12541 agttcgtgct tccgtcaaga aattatgccg taactgcaaa atcgttaagc gtgatggtgt
    12601 catccgtgtg atttgcagtg ccgagccgaa gcataaacag cgccaaggct gattttttcg
    12661 catatttttc ttgcaaagtt gggttgagct ggctagatta gccagccaat cttttgtatg
    12721 tctgtgcgtt tccatttgag tatcctgaaa acgggctttt cagcatggaa cgtacatatt
    12781 aaatagtagg agtgcatagt ggcccgtata gcaggcatta acattcctga tcataagcat
    12841 gccgtaatcg cattaacttc gatttatggc gtcggcaaga cccgttctaa agccatcctg
    12901 gctgcagcgg gtatcgctga agatgttaag atcagtgagc tgtctgaagg acaaatcgac
    12961 acgctgcgtg acgaagttgc caaatttgtc gttgaaggtg atctgcgccg tgaaatcagc
    13021 atgagcatca agcgcctgat ggatcttggt tgctatcgcg gtttgcgtca tcgtcgtggt
    13081 ctcccggttc gcggtcagcg taccaagacc aacgcacgta cccgtaaggg tccgcgcaaa
    13141 ccgatcaaga aataatcggg gtgattgaat aatggcaaag gcaccaattc gtgcacgtaa
    13201 acgtgtaaga aaacaagtct ctgacggcgt ggctcatatc catgcttctt tcaacaacac
    13261 catcgtgact atcactgatc gtcagggtaa cgcgttgggt tgggcaacag ccggtggttc
    13321 cggtttccgt ggttctcgca aatccactcc gtttgcagct caggttgcag cagagcgttg
    13381 cgctgacgcc gtgaaagaat acggcatcaa gaatctggaa gttatggtta aaggtccggg
    13441 tccaggccgc gaatctacta ttcgtgctct gaacgccgca ggtttccgca tcactaatat
    13501 tactgatgtg actccgatcc ctcataacgg ttgtcgtccg ccgaaaaaac gtcgcgtata
    13561 acgcctcgtt ttccaggttt gttggagaaa gaaaatggca agatatttgg gtcctaagct
    13621 caagctgagc cgtcgtgagg gcaccgactt attccttaag tctggcgttc gcgcgatcga
    13681 taccaagtgt aaaattgaac aagctcctgg ccagcacggt gcgcgtaaac cgcgtctgtc
    13741 tgactatggt gtgcagttgc gtgaaaagca aaaagttcgc cgtatctatg gtgtgctgga
    13801 gcgtcagttc cgtaactact acaaagaagc agcacgtctg aaaggcaaca ccggtgaaaa
    13861 cctgttggct ctgctggaag gtcgtctgga caacgttgta taccgtatgg gcttcggtgc
    13921 cactcgtgca gaagcacgtc agctggttag ccataaagca attatggtaa acggtcgtgt
    13981 tgttaacatc gcttcttatc aggttagtcc gaatgacgtt gtaagcattc gtgagaaagc
    14041 gaagaagcag tctcgcgtga aagccgctct ggagctggct gagcagcgtg aaaagccaac
    14101 ctggctggaa gttgatgctg gcaagatgga aggtacgttt aagcgtaagc cggagcgttc
    14161 tgatctgtct gcggacatta acgaacacct gatcgtcgag ctttactcca agtaaagctt
    14221 agtaccaaag agaggacaca atgcagggtt ctgtgacaga gtttctaaaa ccgcgcctgg
    14281 ttgatatcga gcaagtgagt tcgacgcacg ccaaggtgac ccttgagcct ttagagcgtg
    14341 gctttggcca tactctgggt aacgcactgc gccgtattct gctctcatcg atgccgggtt
    14401 gcgcggtgac cgaggttgag attgatggtg tactacatga gtacagcacc aaagaaggcg
    14461 ttcaggaaga tatcctggaa atcctgctca acctgaaagg gctggcggtg agagttcagg
    14521 gcaaagatga agttattctt accttgaata aatctggcat tggccctgtg actgcagccg
    14581 atatcaccca cgacggtgat gtcgaaatcg tcaagccgca gcacgtgatc tgccacctga
    14641 ccgatgagaa cgcgtctatt agcatgcgta tcaaagttca gcgcggtcgt ggttatgtgc
    14701 cggcttctac ccgaattcat tcggaagaag atgagcgccc aatcggccgt ctgctggtcg
    14761 acgcatgcta cagccctgtg gagcgtattg cctacaatgt tgaagcagcg cgtgtagaac
    14821 agcgtaccga cctggacaag ctggtcatcg aaatggaaac caacggcaca atcgatcctg
    14881 aagaggcgat tcgtcgtgcg gcaaccattc tggctgaaca actggaagct ttcgttgact
    14941 tacgtgatgt acgtcagcct gaagtgaaag aagagaaacc agagttcgat ccgatcctgc
    15001 tgcgccctgt tgacgatctg gaattgactg tccgctctgc taactgcctt aaagcagaag
    15061 ctatccacta tatcggtgat ctggtacagc gtaccgaggt tgagctcctt aaaacgccta
    15121 accttggtaa aaaatctctt actgagatta aagacgtgct ggcttcccgt ggactgtctc
    15181 tgggcatgcg cctggaaaac tggccaccgg caagcatcgc tgacgagtaa ccggatcaca
    15241 ggttaaggtt ttactgagaa ggataaggtc atgcgccatc gtaagagtgg tcgtcaactg
    15301 aaccgcaaca gcagccatcg ccaggctatg ttccgcaata tggcaggttc actggttcgt
    15361 catgaaatca tcaagacgac tctgcctaaa gcgaaagagc tgcgccgcgt agttgagccg
    15421 ctgattactc ttgccaagac tgatagcgtt gctaatcgtc gtctggcatt cgcccgtact
    15481 cgtgataacg agatcgtggc aaaactgttt aacgaactgg gcccgcgttt cgcgagccgt
    15541 gccggtggtt acactcgtat tctgaagtgt ggcttccgtg caggcgacaa cgcgccgatg
    15601 gcttacatcg agctggttga tcgttcagag aaagcagaag ctgctgcaga gtaatctgaa
    15661 gcaacgtaaa aaaacccgcc ccggcgggtt tttttatacc cgtagcatcc ccacttatct
    15721 acaatagctg tactcttttt gttcatcccc tggagtattt atgtggttac ttgaccagtg
    15781 ggcagagcgc catatagcag aagcgcaagc gaaaggtgag tttgataacc tggcaggtgg
    15841 cggcgaacca ttgatactgg atgatgattc tcacgtgcca ccggaattac gtgcggggta
    15901 tcgcttgctg aagaatgccg gttgcttacc gccagaactt gagcaacgga gagaagcaat
    15961 tcagcttctg gatattctca aaggtatccg tcacgatgat ccgcaatatc aagaggttag
    16021 ccgtcgattg tcattactgg aattgaagct gcgacaagct ggattgagta ccgatttttt
    16081 acgcggcgat tatgctgaca agttgttgga caaaatcaac gataactagt ggagtatgta
    16141 tgtatcgcat tggtgagctg gcaaaaatgg cggaagtaac acccgacacg attcgttatt
    16201 acgaaaaaca gcagatgatg gagcatgaag tgcgtactga aggtgggttt cgcctgtata
    16261 ccgaaagcga tctccagcga ttgaaattta tccgccatgc cagacaacta ggtttcagtc
    16321 tggagtcgat ccgcgagttg ctgtcgatcc gtatcgatcc tgaacaccat acctgtcagg
    16381 agtcaaaagg cattgtgcag gaaagattgc aggaagtcga agcacggata gccgagttgc
    16441 agagtatgca gcgttccttg caacgcctta acgatgcctg ttgtgggacc gcccatagca
    16501 gtgtttattg ctcgattctt gaagctcttg aacaaggggc gagtggcgtt aagagtggct
    16561 gttgattttt tgcactgaca ggattacact cgcgccgtta aataaccaac tggagttttt
    16621 atgagccgat atcagcatac taaagggcag ataaaggata atgcgataga agcattacta
    16681 catgatccct tattccgaca gcgagtagag aaaaataaga aagggaaagg cagttacatg
    16741 cgaaaaggaa aacatggcaa tcgggggaac tgggaggcca gtggcaagaa agtaaatcac
    16801 ttttttacca ctggccttct gctttcaggc gtttgttaag agcggttatt ctgctctttc
    16861 agcaaatcac gaatttctgt cagtaatact tcttctttag ttggtgctgg tgcggctgct
    16921 ggttcttctt ttttccgatt cagtttgttg attagcttaa tcgccataaa gatggcaaag
    16981 gccacaatca gaaaatcaaa gacgttttga atgaagacac cgtaatgcat cacaacagca
    17041 gggatatccc cctgcgcatc gcgtagcgtg acagcaaact gtttaaagtc gatcccgcca
    17101 attaataagc ccagaggtgg catgatgata tcggcaacca gtgaagatac aatcttcccg
    17161 aatgccgcac cgataatgac acccaccgcc aaatccacca cgttcccgcg catcgcaaat
    17221 tcgcgaaatt ctttaataat gctcatgtta ttctccctat gaagccgaca accataagtc
    17281 taacaaatgt taagccattt tcctgccagg aatgaattta attaatcctt gataatcaaa
    17341 gggaaataat aaggcgtcat tagacgcctt attaattaca agaagaaagg gcttggctgg
    17401 aagagtcttt cgacgtcggt aataaacttt ttatctgtga ggaacataat tacgtgatcg
    17461 ccttgctcaa tgcgcagatt gtcattggca atcatcacgt cgtttccacg taccaccgct
    17521 ccaataatcg ttcctggcgg tagcttgatt tcgtcaatga ctctgccgac aacgcgtgag
    17581 gtgctttcat caccgtgagc aacggcttca atagcttctg ctacgccgcg gcgcaatgag
    17641 gaaacaccaa caatatctgc ttttcgcaca tggctaagca acgcagaaat agttgcttgt
    17701 tgtggtgaaa tcgcaatatc gatcacgctc ccctgaacca gatccacata agcgcgacgc
    17761 tggatcaata ccatcacctt tttcgcaccc atacgtttgg caagcatggc ggacatgata
    17821 ttggcctcgt catcgttggt gacggcaata aacagatcaa cttgatcgat atgttcttcg
    17881 gccagcagtt cttgatccga cgcatcacca aaaaagacga tcgtattctg caacttttcc
    17941 gccagttcgg cagcgcgctg ctgattacgt tcgatgagtt taacgctgta atctttttcc
    18001 agacgacgcg ccagccctgc accgatatta ccgccaccaa ccagcatgat ccgcttatac
    18061 ggtttttcca gtcgctgtaa ttcactcatc accgcacgga tatgctgtga agcggcaata
    18121 aagaacactt catcaccagc ttccacaatg gtcgaacctt gtggacgaat ggggcgatcg
    18181 tggcggaaaa ttgctgccac acgagtatcg atatgtggca tatgttcgcg catggtcgaa
    18241 agtgcgttac caatcagcgg gccgccataa taggctttaa ccacagcgag gctgacttta
    18301 ccctcagcga agttcaccac ctgcaatgcg ccgggatact caatcagtcg gtaaatatta
    18361 tcgataacca actgctctgg tgcgatcaga tgatcaatcg gtacagcatc tgaatgaaat
    18421 agcttatcgg catcgcgcac gtagtctggt gagcggatgc gagcgatgcg attaggggtg
    18481 ttgaaaagtg agtaggctac ctggcaggca accatattgg tttcatctga actggttaca
    18541 gcaaccagca tatcggcgtc gtcggcacct gcctcccgca atacgcgtgg atgagagcca
    18601 tgcccctgca cgactcgcag gtcaaattta tcctgcaagg tccgcagacg ctcaccgttg
    18661 gtatcgacaa cagtgatatc gttgttctcg ccaaccaggt tttccgccag tgtgccgcca
    18721 acctggccgg cacccagaat gataattttc atcagtcgcg acccgttatc tcatcacttt
    18781 ttgattagct tagcgtaaaa gaagccgtcg ccctcttcgg caccaggcag attttgttta
    18841 cccggttgct ctggtgttcc tgtttcgcaa agttcggcat cagcggtacg ttgcaaaaag
    18901 gctttaatct gcaggctatt ctcttccggt aacaccgaac aggtggcata gaccagggtt
    18961 ccaccggatt ttaaatgcgg ccaaatggcg tcgagaattt cagactgcaa ttgcgcgagt
    19021 tccgggatat cgcgatcgcg acgtaaccat ttaatatccg gatggcgacg aatcacaccg
    19081 gttgctgaac aaggcgcatc taataaaatg cgatcaaact gttgctcgcc acaccattgg
    19141 gaagggtagc ggccatcacc ttgtttcacg gtcgctttca taccaaggcg ttttaaattg
    19201 tcgtaaacgc gagagaggcg ctgttcgtca atatcaaccg caacaacctg cgcttctggt
    19261 gccacctcaa ggatatgcgt tgttttaccg ccgggggcgg cacaaagatc cagaatgtgt
    19321 tcaccgtttt gtggcgcaag ccaggtcatg caaccttgtg ctgatgcatc ctgaacggta
    19381 acccatccgt cttcaaaacc aggtagcgca tgaacaggtg caggtgtttc cagccgtaca
    19441 gcatcagggt aatccgcatg cgggaaacct ttcattcctg cttcatccaa caatgcaagc
    19501 cagctgtcgc gggaatgatg cgtacgatta acgcgcagcc acattggcgg acgctggtta
    19561 ttggcttcga cgatggattg ccactgctct ggatacgctt tttgcagacg cttcagcaac
    19621 caggaaggat gcagataacg tgcatcactg gcattaaact cggctaataa ctcttcttgc
    19681 tgacgctgga actggcgtaa tacaccgttt atcaaccctt taagttgcgg acgcttaatt
    19741 gcgatagcgc cttcaaccgt ttcagccagc gcagcatgag gtggaatgcg ggtataaagc
    19801 agttgataca aaccaaccat aatcaggtaa tgcacggtcc gctgtttgcc ggtcatcgga
    19861 cgggccatta acttattaat cagccagtct aactgcgaaa gcgtacgcag tacgccaaag
    19921 cacaactctt gaagaagtgc tttgtcttta tcggaaactt tttgctggag cggtggcaga
    19981 atgttgctta atgattgccc ttgctcgacg acttgttcaa cggcctgggc cgccatgcta
    20041 cgtaaattac gttgtttttt cataagtata aaaatgcccg gcaagaccgg gcttagaaga
    20101 gtggactatc agaccagacg gttgcccgga acaaaccatt cccgacgaga gttcaggagg
    20161 tcttgcgcgc tcatcgcttt cttacccgca ggttgtaacg agagcaggtt caggatgcca
    20221 tcaccagtcg caacctgaat gccttgttta ttggcttcaa ggatcgttcc tggtgcagcg
    20281 ttggttgccg tatcaatgac cgacgctttc cagactttaa ccggttgtcc ttcaatttcc
    20341 agccagctca ttggccatgg gttgaaagcg cgaatgcagc gttcaagctg cgctgccgaa
    20401 agcgaccagt caatacgcgc ttcttcttta ctcaacttct cggcgtaagt gacaagagtt
    20461 tcgtcctgaa cttctggttt cgccgtgccg tctgccagtt gtttcaacgt ggtgataagc
    20521 ccttgtgggc caagctctgc cagcttgtcg tacagcgtac cactggtatc ttctgcagta
    20581 atcgggcagg agagcttata gagcatgtca ccggtgtcta aaccaacatc catttgcata
    20641 atggtcacac cagtttctgc atcacccgcc catagtgagc gttggattgg tgcagcaccg
    20701 cgccagcgtg gcagcagtga accatgaacg ttgatacagc caagacgcgg catctccagc
    20761 acagctttag gcagaattaa accataggcg acgacgacca taacatcagc ctgcagatcg
    20821 gcgaccagtt gctggttttc ttgtggacgc agggaaacag gttgaaaaac gggcagacct
    20881 ttttcctcag ccagaacttt aatcgggctg ggcatcagtt ttttaccgcg tcctgccggt
    20941 cggtctggct gggtgaacac gccaacgacg ttatgaccag aagataacag cgcgtcgaga
    21001 tgacgcgctg caaagtcagg tgtacccgca aaaataatac gtagtgattc tgacacgtta
    21061 gttcttatcc ttaagcccgg gctttcagac gatccagttt ttcaactttc tgacgaatac
    21121 gttgttgttt cagcggtgac agataatcca taaacagttt gccgaccagg tgatccatct
    21181 catgctgaat acagatggct aacagaccat ctgcctccag ttcaaatggt ttaccgtcgc
    21241 ggtccagggc gcgaatttta actttctctg cgcgtggcac taaagcacgt tgttcaggga
    21301 tcgacaggca accttcttca atgcctgttt cgccgctttt ttctaaaagc tccggattga
    21361 ttaacaccag ccgttcgtca cggttttccg aaacatcaat aacaatgata cgttgatgga
    21421 tatcaacctg ggttgccgcc aggccaatac cttcttctgc atacatcgtc tcgaacatat
    21481 catcgacgat acgctgaatt tctgcattca cttcttctac cggtttagca actttgcgaa
    21541 gccgctcgtc cggaatatgt aacacttgca aaactgacat aaatctccag agatgtgttc
    21601 aggagttaga aagattattt cttctattct agacaaatcc ccctctgatt gacagcatca
    21661 ctgaccaatc gcaaagattg ctaaggctgc ttatggcagg gagataagga tggtcgatac
    21721 agaaatgtgg ctgcgtttaa tgagtatcag cagcttgtac ggcgatgata tggtccgtat
    21781 agctcactgg ctggcaaaac agtcgcatat tgatgcggtt gtattgcagc aaactgggct
    21841 tacattgcgg caggcacaac gctttctttc atttccgcga aagagtatcg aaagctcact
    21901 ttgttggttg gagcaaccca accatcattt aatccctgcg gacagcgaat tttatcctcc
    21961 tcaacttcag gcgacgacag attaccccgg cgcactgttt gttgaaggag aactgcacgc
    22021 gctgcattca tttcagcttg ccatagtggg gagtcgggcg cattcatggt atggcgagcg
    22081 atggggacga ttattttgcg aaactctggc gacgcgtgga gtgacaatta caagtggact
    22141 ggcgcgtgga atcgatggtg tggcgcataa agcggcctta caggtaaatg gcgtcagcat
    22201 tgctgtattg gggaatggac ttaataccat tcatccccgc cgccatgccc gactggctgc
    22261 cagtttgctt gaacatggcg gagctctcgt ctcggaattt cccctcgatg ttccacccct
    22321 tgcttacaat ttcccacgaa gaaatcgcat tatcagtggt ctaagtaaag gtgtactggt
    22381 ggtggaagcg gctttgcgca gtggttcgct agtgacagca cgttgtgcgc ttgagcaggg
    22441 gcgtgaagtt tttgccttgc caggtccaat agggaatccg ggaagcgaag ggcctcactg
    22501 gttaataaaa caaggtgcga ttcttgtgac ggaaccggaa gaaattctgg aaaacttgca
    22561 atttggattg cactggttgc cagacgcccc tgaaaattca ttttattcac cagatcagca
    22621 agacgtggca ttgccatttc ctgagctcct ggctaacgta ggagatgagg taacacctgt
    22681 tgacgtcgtc gctgaacgtg ccggccaacc tgtgccagag gtagttactc aactactcga
    22741 actggagtta gcaggatgga tcgcagctgt acccggcggc tatgtccgat tgaggagggc
    22801 atgccatgtt cgacgtacta atgtatttgt ttgaaaccta tattcacaca gaagctgagt
    22861 tgcgtgtgga tcaagacaaa cttgaacagg atcttaccga cgcaggattt gatcgagaag
    22921 atatctacaa tgccctgctt tggctggaaa agcttgcgga ttatcaggaa ggactggcag
    22981 aaccgatgca actggcctct gatccactct ccatgcgtat ttatacccct gaggagtgtg
    23041 aaagactgga tgccagctgt cgtggttttc tgcttttcct tgagcagatt caggtgctca
    23101 accttgaaac tcgtgaaatg gtgatagagc gagtgctcgc gctggataac gcagagttcg
    23161 aactggatga tctgaaatgg gtaatcctga tggtgttgtt caatattccg ggctgcgaaa
    23221 atgcgtacca gcaaatggaa gaattactct ttgaagtgaa tgaaggtatg ctgcattaat
    23281 catttcttta attcagcata agttgttatg gcgaaatcag cactgttcac ggtgcgtaat
    23341 aatgagtcct gcccaaagtg cggggctgaa ctggttattc gatccgggaa acacggtccg
    23401 tttcttggat gctcacagta tccggcgtgt gactacgtcc gtcctctgaa atcttcagcg
    23461 gatggacata ttgtcaaagt tctggagggg caggtttgcc ctgcatgtgg cgcaaatctg
    23521 gtattacgcc agggacgctt tggtatgttt attggttgca gtaactaccc tgaatgcgaa
    23581 cataccgaac ttatcgataa accggacgaa acagcaatta catgccccca atgccggacg
    23641 ggccatctgg tccagcgccg ctcccgttat ggcaaaacat ttcactcctg tgatcgctac
    23701 ccggagtgtc aatttgccat taacttcaaa cccatagctg gagaatgccc tgagtgtcat
    23761 tatccactac tcatcgaaaa gaaaaccgcg cagggtgtga aacacttttg tgccagtaaa
    23821 caatgtggaa agccggtttc ggcggaataa taacgtgaat aataacctgc aaggagacgc
    23881 tatcgcagct gcgatagatg tcctcaatga agaacgtgtc atcgcctatc caacggaagc
    23941 cgttttcggt gtcgggtgcg atcctgatag cgaaacagca gtgatgcgac tgttggagtt
    24001 aaaacagcgt ccggttgata aggggctgat tttaatcgcg gcaaattacg agcagcttaa
    24061 accctatatt gatgacacca tgctgactga tgcgcagcgt gaaaccattt tttcccgctg
    24121 gccaggtcct gtcacctttg tctttcccgc gcctgcgaca acaccgcgct ggttgacggg
    24181 ccgctttgat tcgcttgctg tacgagtcac cgaccatccg ttggtggttg ctttgtgcca
    24241 ggcttatggt aaaccgctgg tttctaccag tgccaacttg agtggattgc caccttgtcg
    24301 aacagtagac gaagttcgcg cacaatttgg cgcggcgttc ccggttgtgc ctggtgaaac
    24361 gggggggcgt ttaaatcctt cagaaatccg cgatgccctg acgggtgaac tgtttcgaca
    24421 ggggtaacat aatggaaacc tatgctgttt ttggtaatcc gatagcccac agcaaatcgc
    24481 cattcattca tcagcaattt gctcagcaac tgaatattga acatccctat gggcgcgtgt
    24541 tggcacccat caatgatttc atcaacacac tgaacgcttt ctttcgtgct ggtggtaaag
    24601 gtgcgaatgt gacggtgcct tttaaagaag aggcttttgc cagagcggat gagcttactg
    24661 aacgggcagc gttggctggt gctgttaata ccctcaagcg gttagaagat ggacgcctgc
    24721 tgggtgacaa taccgatggt ataggtttgt taagcgatct ggaacgtctg tcttttatcc
    24781 gtcctggttt acgtattctg ctcatcggcg ctggtggagc atctcgcggc gtactactgc
    24841 cacttctttc cctggactgt gcggtgacaa tcactaatcg gacggtatcc cgcgcggaag
    24901 agttggctaa attgtttgcg cacactggca gtattcaggc gttgggtatg gacgaactgg
    24961 aaggtcatga gtttgatctc attattaatg caacatccag tggcatcagt ggtgatattc
    25021 cggcgatccc gtcatcgctc attcatccag gcatttattg ctatgacatg ttctatcaga
    25081 aaggaaaaac tccttttctg gcatggtgtg agcagcgagg ctcaaagcgt aatgctgatg
    25141 gtttaggaat gctggtggca caggcggctc atgcctttct tctctggcac ggtgttctgc
    25201 ctgacgtaga accagttata aagcaattgc aggaggaatt gtccgcgtga atcaggccat
    25261 ccagtttccg gacagagaag agtgggacga gaataaaaaa tgtgtatgtt ttcccgctct
    25321 cgtgaatggc atgcaactga catgtgcgat ctctggcgag agtctggcgt atcgttttac
    25381 tggagatacg ccagaacagt ggttagcgag ttttcgtcag catcgctggg atctggaaga
    25441 agaagcggaa aacttaattc aggaacaaag tgaagatgat caaggctggg tctggttacc
    25501 ctgatccaga tattcgtcct tccatttcac gtaattattc gcggaatagc gtaacccagc
    25561 cttctcttca tcacttaacg ggcggatctg tttgacgggg ctaccgagat acagatatcc
    25621 gctctccagc cgtttatttt gtgggaccag actacccgca ccaatcatca catcatcttc
    25681 tactattgcg ccatcaagta aaattgagcc catcccaacc aaaactcgat tgccaatggt
    25741 gcagccgtgg agcatcacct tgtgaccaac agtgacatct tcgccaatgg ttaatgggtt
    25801 gccatctggg ttgtacgagg atttatgagt gacatgcaac atactgccat cctggatatt
    25861 ggtgcgtgct ccgatctgta cataatgtac atctccacga atcacaacga gcggccagat
    25921 ccccacatca tcagccagac gaacgtcacc aatcacgaca ctgctatcgt cgatcattac
    25981 gcgctgaccg atttgtggaa aaagatcgcg gtatgggcgt aaaacatcag acatacttac
    26041 ctcagcaata aatgatttac taatgacttt gggggcatta ttggccttgt gcaagtcttt
    26101 tagtatgcaa aaaagcaccg ttttgtgtgc gaatgcggca aaaagggtga aaaaacaaca
    26161 aacagaaaaa aagatcaaaa aagtacttgt gcaaaaaatt gggatcccta taatgcgcct
    26221 ccgttgagac gacaacgtga aacacttcac aagatggtcg taacaacgaa gag
//
LOCUS       DAZGSP010000071        25156 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZGSP010000071 DAZGSP010000000
VERSION     DAZGSP010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.071"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(145..627)
                     /gene="ybiA"
                     /locus_tag="ACHK74_003251"
     CDS             complement(145..627)
                     /gene="ybiA"
                     /locus_tag="ACHK74_003251"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFP0222017.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRERSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            747..2897
                     /gene="dinG"
                     /locus_tag="ACHK74_003252"
     CDS             747..2897
                     /gene="dinG"
                     /locus_tag="ACHK74_003252"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFP0222018.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGEHSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            2925..3887
                     /gene="ybiB"
                     /locus_tag="ACHK74_003253"
     CDS             2925..3887
                     /gene="ybiB"
                     /locus_tag="ACHK74_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFP0222019.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            4028..5113
                     /gene="hcxB"
                     /locus_tag="ACHK74_003254"
     CDS             4028..5113
                     /gene="hcxB"
                     /locus_tag="ACHK74_003254"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFP0222020.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5180..5239
                     /gene="ybiE"
                     /locus_tag="ACHK74_003255"
     CDS             5180..5239
                     /gene="ybiE"
                     /locus_tag="ACHK74_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFP0222021.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5342..5602)
                     /gene="ybiJ"
                     /locus_tag="ACHK74_003256"
     CDS             complement(5342..5602)
                     /gene="ybiJ"
                     /locus_tag="ACHK74_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFP0222022.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(5867..6133)
                     /gene="ybiI"
                     /locus_tag="ACHK74_003257"
     CDS             complement(5867..6133)
                     /gene="ybiI"
                     /locus_tag="ACHK74_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFP0222023.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNLRGSKDSQLR"
     gene            complement(6207..6884)
                     /gene="ybiX"
                     /locus_tag="ACHK74_003258"
     CDS             complement(6207..6884)
                     /gene="ybiX"
                     /locus_tag="ACHK74_003258"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFP0222024.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(6926..9208)
                     /gene="fiu"
                     /locus_tag="ACHK74_003259"
     CDS             complement(6926..9208)
                     /gene="fiu"
                     /locus_tag="ACHK74_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFP0222025.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(9473..9733)
                     /gene="mcbA"
                     /locus_tag="ACHK74_003260"
     CDS             complement(9473..9733)
                     /gene="mcbA"
                     /locus_tag="ACHK74_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFP0222026.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            10009..10935
                     /gene="rlmF"
                     /locus_tag="ACHK74_003261"
     CDS             10009..10935
                     /gene="rlmF"
                     /locus_tag="ACHK74_003261"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFP0222027.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWYEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(10932..13157)
                     /gene="ybiO"
                     /locus_tag="ACHK74_003262"
     CDS             complement(10932..13157)
                     /gene="ybiO"
                     /locus_tag="ACHK74_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFP0222028.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(13274..13996)
                     /gene="glnQ"
                     /locus_tag="ACHK74_003263"
     CDS             complement(13274..13996)
                     /gene="glnQ"
                     /locus_tag="ACHK74_003263"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFP0222029.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(13993..14652)
                     /gene="glnP"
                     /locus_tag="ACHK74_003264"
     CDS             complement(13993..14652)
                     /gene="glnP"
                     /locus_tag="ACHK74_003264"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFP0222030.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(14791..15537)
                     /gene="glnH"
                     /locus_tag="ACHK74_003265"
     CDS             complement(14791..15537)
                     /gene="glnH"
                     /locus_tag="ACHK74_003265"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFP0222031.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(15941..16444)
                     /gene="dps"
                     /locus_tag="ACHK74_003266"
                     /gene_synonym="pexB"
     CDS             complement(15941..16444)
                     /gene="dps"
                     /locus_tag="ACHK74_003266"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFP0222032.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(16743..17630)
                     /gene="rhtA"
                     /locus_tag="ACHK74_003267"
     CDS             complement(16743..17630)
                     /gene="rhtA"
                     /locus_tag="ACHK74_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFP0222033.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            17865..17930
                     /locus_tag="ACHK74_003268"
     CDS             17865..17930
                     /locus_tag="ACHK74_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222034.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            17983..18498
                     /gene="ompX"
                     /locus_tag="ACHK74_003269"
     CDS             17983..18498
                     /gene="ompX"
                     /locus_tag="ACHK74_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFP0222035.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(18547..20130)
                     /gene="opgE"
                     /locus_tag="ACHK74_003270"
     CDS             complement(18547..20130)
                     /gene="opgE"
                     /locus_tag="ACHK74_003270"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP0222036.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(20402..20530)
                     /gene="mntS"
                     /locus_tag="ACHK74_003271"
     CDS             complement(20402..20530)
                     /gene="mntS"
                     /locus_tag="ACHK74_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFP0222037.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20716..21183
                     /gene="mntR"
                     /locus_tag="ACHK74_003272"
     CDS             20716..21183
                     /gene="mntR"
                     /locus_tag="ACHK74_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFP0222038.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            21180..22298
                     /gene="ybiR"
                     /locus_tag="ACHK74_003273"
     CDS             21180..22298
                     /gene="ybiR"
                     /locus_tag="ACHK74_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFP0222039.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(22357..23277)
                     /gene="ldtB"
                     /locus_tag="ACHK74_003274"
     CDS             complement(22357..23277)
                     /gene="ldtB"
                     /locus_tag="ACHK74_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP0222040.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            23496..25088
                     /gene="ybiT"
                     /locus_tag="ACHK74_003275"
     CDS             23496..25088
                     /gene="ybiT"
                     /locus_tag="ACHK74_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFP0222041.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
BASE COUNT         6127 a         6210 c         6694 g         6125 t
ORIGIN      
        1 tctgcaatat attgaatttg catgcttttg taggccggat aaggcgttta cgccgcatcc
       61 ggcatttcac aacaagcact tgtcagcaat ttgagaacac gggaaaaaat ttatctgttt
      121 taccgcccat aagccaccga gacgttactt ctctatagcc aattgttcgc gcaactccat
      181 taaaaggtag cccagtcgat tcttgccctt accattacca ccgtctcccc agtaagcatc
      241 gttttccgta tgctcaacca gttttgcggg cgcggttgcc agcaagagcg cacgcagttc
      301 tggatgctgt tcgaatttag cgcgaagtgc ttttcgcatc acctgttctt tgaccgactc
      361 ccagttttta cgcagaggct tagaacgctc gcgccccatg cgtgcggcga ccatagggga
      421 agaaacccga cggatctctt cgcggtattt ttcatcaagg aatttttgtg cctgaaaata
      481 gtgttctgag gtgggccagg ttttcccgtc aaccttgatg ggccaggcgg caaagttaga
      541 aaaatcaccg tagtcatcgc tggtgctgta gaaatttatg atggtgtctt gcatgacgtg
      601 ttggatcctt tgtgctcgaa cgggcattaa accgcattat gttggtggtt attgcgagcc
      661 gctttccaga aacagaaaaa ccattacccc tgaaaaccga aaaatgccac aatattggct
      721 gtttatacag tatttcaggt tttctcatgg cattaaccgc cgcgcttaaa gcgcaaattg
      781 ccgcctggta taaggcgctt caggaacaga tccccgactt tattccccgt gcgccgcagc
      841 ggcagatgat tgcggacgtc gccaaaacgc tggccggaga agaagggcgg catctggcga
      901 ttgaagcccc caccggcgtt gggaaaacgc tctcctattt gatccccggc atcgccattg
      961 cccgcgaaga gcaaaaaacg ctggtggtga gtaccgccaa cgtggcgttg caggatcaga
     1021 tttacagcaa agatttaccg ctgctgaaaa agatcattcc cgatcttaaa ttcactgccg
     1081 cttttgggcg tgggcgctac gtttgcccgc gtaatctgac ggcgctcgcc agcactgagc
     1141 ccacgcaaca ggatctgctg gcgtttcttg acgacgaact gacgccaaat aatcaggaag
     1201 agcaaaaacg ttgtgcgaag ctgaagggcg atctcgacac ttataaatgg gatggtctgc
     1261 gcgatcatac tgatatcgct atagatgacg atctctggcg tcgtttaagt accgacaaag
     1321 ccagctgcct caaccgcaac tgttactact atcgtgaatg cccgtttttt gttgctcgtc
     1381 gggagattca ggaagcggaa gtggtggtgg caaaccatgc gctggtgatg gcggcgatgg
     1441 aaagcgaagc cgtactacct gacccgaaaa atctgctgct ggtgctggac gaaggtcatc
     1501 acctgccaga tgtggcccgg gatgcgctgg agatgagtgc cgaaatcacc gcgccctggt
     1561 atcgactaca gctggacttg ttcacgaaac tggtcgctac ctgcatggag cagtttcgcc
     1621 cgaagaccat cccaccgctg gcgatccctg aacgtttgaa tgcgcattgt gaagagttgt
     1681 atgagcttat cgcctcgtta aacaacattc tcaatctcta catgcctgcc gggcaggagg
     1741 cagagcaccg ttttgcgatg ggcgaactgc ctgatgaagt gctggagatc tgccagcggc
     1801 tggcaaaact caccgagatg ctgcgtggtc tggcggagtt atttcttaac gatttaagtg
     1861 agaaaaccgg cagccacgat attgtgcgtc tgcatcggtt gattttgcag atgaaccgcg
     1921 cgttggggat gttcgaggcg caaagcaaac tctggcggct ggcgtctctg gcgcaatctt
     1981 ccggtgcgcc ggtgaccaaa tgggcgacgc gggaagagcg cgaagggcag ctgcatctct
     2041 ggtttcactg cgtggggata cgtgtcagcg atcagctgga aaggctgctg tggcgcagta
     2101 ttccgcacat tattgttacc tccgcaacct tgcgttcgct gaatagtttt tcgcgtttgc
     2161 aggagatgag tggtctgaaa gagaaagcgg gcgaccgttt tgtggcgctg gattccccct
     2221 ttaaccactg cgaacagggc aaaattgtta ttccccggat gcgcgttgag ccttccatcg
     2281 acaacgaaga gcagcatatt gccgaaatgg cggccttttt ccgtaagcag gtggagagca
     2341 aaaaacatct cggtatgttg gtactgtttg ccagcggacg ggcgatgcag cgctttctcg
     2401 actatgtgac ggatttacgt ctgatgctgc tggtgcaggg cgatcagccg cgttatcgct
     2461 tagttgaact gcaccgcaaa cgcgttgcca acggtgagca tagtgtgctg gtgggcttac
     2521 agtcatttgc cgaagggctt gatttgaaag gtgatctgct cagccaggtg catatccaca
     2581 aaatcgcttt tccgcccatc gacagcccgg tggtgatcac cgaaggggaa tggctgaaaa
     2641 gcctcaaccg ctatccgttt gaggtgcaaa gcctgccgag cgcctcgttt aacctgattc
     2701 agcaggttgg gcgactgatt cgaagccacg gttgctgggg cgaagtggtt atctacgaca
     2761 aacgcttgct gaccaaaaat tatggcaagc gactactgga tgcattaccg gtatttccga
     2821 tagagcaacc ggaagtccct gaaggtatag ttaaaaagaa agaaaaaacg aaatccccac
     2881 gccgtcggcg gcgttaatga tgtgagtcag gtaaggagtc gtaaatggac tatcgcaaaa
     2941 tcattaaaga gatcgggcgc gggaaaaacc acgcgcgcga tttagaccgg gatactgcgc
     3001 gcggtctgta tgctcatatg ctcaatggtg aagtccctga ccttgagttg ggcggcgtat
     3061 tgattgcgct gcgtatcaaa ggagaagggg aagcagagat gctcggcttt tacgaagcca
     3121 tgcaaaatca caccatcaag ctgacgccgc cagcaggcaa gccgatgccg attgtcatcc
     3181 ccagttacaa cggcgcgcgt aaacaggcca acctgacgcc gttgttggcg attctcctgc
     3241 acaaactcgg ttttcctgtg gtggttcacg gggttagcga agatccaacc cgcgtgctga
     3301 ctgaaaccat ttttgaattg atgggtatta cgccaacgtt gcacggcggg caggcgcagg
     3361 cgaagctcga cgaacatcaa ccggtgttta tgccagtcgg cgcgttttgc ccgccgctgg
     3421 aaaaacaact ggcgatgcgc tggcggatgg gcgtgcgtaa cagtgcacat accctggcga
     3481 aactggcgac gccatttgcc gaaggtgagg cgctgcgtct ttccagcgtt tcgcatccgg
     3541 aatacattgg acgcgtcgcg aagttcttta gcgatatcgg cgggcgggcg ctactaatgc
     3601 atggtactga aggtgaagtg tatgctaatc cgcagcgctg cccgcagatc aatctcattg
     3661 accgtgaagg gatgcgggtg ctgtatgaaa aacaggacac tgctggtagc gagttactgc
     3721 cacaagcaaa agatccggaa accacggcgc aatggattga gcgttgcctt gctggcagcg
     3781 aaccgattcc cgaatcgctg aaaatccaga tggcttgctg cctggtggct acgggtgaag
     3841 cggcaactat cagcgacggc ctggcgcgcg ttaatcaggc attttaattc ttttctcagc
     3901 cggatgagcc atgtcatccg gcttttaccc caccgcaata tgaaattcct gcatctttat
     3961 tgaccttccc acgcccggcg tgcagcataa aaatacaaca aacacataac ataaacagga
     4021 gttaaccatg gaaagtggtc atcgctttga tgctcagacg ctgcacagtt ttattcaggc
     4081 tgtatttcgt cagatgggta gcgaggaaca agaagcgaaa ttagttgccg atcatttaat
     4141 cgcggcaaac ctggcagggc atgattcaca tggtattggc atgatcccaa gctatgtacg
     4201 ctcctggagt caggggcacc tgcaaattaa ccatcatgcc aaaaccgtta aagaggcggg
     4261 ggcggcggtc acgctcgatg gcgatcgcgc atttggtcag gtcgcggcac atgaagcgat
     4321 ggcgctgggg attgagaaag cgcatcagca cggtattgcc gccgtggcgc tacataactc
     4381 gcatcatatc ggccgtatcg gttactgggc ggagcagtgt gcagcggcgg ggtttgtctc
     4441 tatccacttt gttagcgtgg tcggtattcc aatggtcgcg ccgttccacg gtcgcgacag
     4501 ccgctttggc accaatccgt tctgtgtggt tttccctcgt aaagataatt tcccgctgtt
     4561 gcttgattac gccaccagcg ccattgcatt tggcaaaacc cgcgtcgcct ggcataaagg
     4621 cgtccccgtg ccgccaggtt gcctgattga cgttaacggc gtgccgacga ccaatccggc
     4681 ggtaatgcag gagtcgccgt tgggttcgct gttgaccttt gccgaacata aaggctacgc
     4741 ccttgcagcg atgtgtgaaa ttcttggcgg ggcgctttcc ggcggtaaaa cgacgcatca
     4801 ggaaacgtta caaaccagtc ccgatgccat tcttaactgc atgaccacta tcatcatcaa
     4861 cccggaactg ttcggcgcgc cggattgtaa cgcgcagacc gaagcctttg ccgagtgggt
     4921 gaaagcctcg ccgcatgatg atgataagcc gattttgcta ccgggcgagt gggaagtgaa
     4981 cacgcgtcgc gaacggcaga agcaggggat tccactggat gcgggaagct ggcaggccat
     5041 ttgtgatgca gcgcggcaga ttggtatgcc ggaagagacg ttgcaggctt tctgtcagca
     5101 gttagccagc taaaaaaaag cccgtccagt ggcggacggg caaacaaggg taacatagga
     5161 tcaatgaggg ttagagcata tgcgtctgtc ggcaaacaga cagggaaata cttgtgctgg
     5221 acgtagcgta aacgcctgat ccgtccaacg gttcggattt tgtaggcctg ataagacgcg
     5281 gtagcgtcgc atcaggcatc tgttgccgga tgcggcgtaa acgccttatc cttcctacga
     5341 attacttata aattaccgca gtaccgctta atttattgtt gttggtggcg gaagtgatgc
     5401 tgtatccact ggcaccggct gccgcggctt tctcagccag tttcgcttcc agggcatcga
     5461 gggtggatgc gccatcggca gaaaccacgc cgattttatt catgttctgt gcctgggatg
     5521 ccgttaccgg ttccgcggca aacacgccaa atgacagagt tgaaagagcc atagcagcaa
     5581 caacagtatt gatagttttc ataattaatc tctcgcaggt gatttttgta taagtgacgt
     5641 tgtttcgtcg atgtgatgag tatcacgttt ttttacgaga gataaaatcg aagagaattg
     5701 accgccttgt tcaaataaat tgattgatat ctaacatact gataaaccat ttatttgttg
     5761 tacttatgga cttgttttat gcagcgtggc agtcacgaca gcgttaaagg cacggaatgc
     5821 cacgcaaaat acaaattata ctcgctatac atctcgccag ttgtagttaa cgtaactggc
     5881 tgtctttcga acctctgaga ttatatcctg tatacgctgg tttttgtaaa tctttctcct
     5941 gctgacaatg aatgcataag cgcacgccag gaatggcttc ccgacgggct tgcgggatgg
     6001 gggcaccgca ctcttcacat tcatccaggc tttcgccacg cggaatttca ccccgagcgc
     6061 gggcaatcgc atcttcaatt gtactgttga tctgttcgtt gacggcgtca tcgttagccc
     6121 aaccggatgc catatcgtcc tccccatatc aatgcttgta cagttaagtg tagctaatcc
     6181 agggacgaac ttgggcagtt caagcatcag atctccgacc attcccgcag cagattatga
     6241 taaagattaa gcagcgacag gatctcttca ttttcaccat agcggctttt cagcgactga
     6301 atattgttgt ccagttcaaa cagcatggcg cgctttttat catcacggat catcgactgg
     6361 atccacataa atgatgccac tcgtacgccg cgggttacgg gtgtcacgca atgcaggctg
     6421 ctggagggat acaacacgag atcgcctgcc gggagtttta cccgatgttg tccgaaggtg
     6481 tcattaacga ccagttcgcc gccgtcgtag ctttgtggat cgcttaaaaa cagcgtggca
     6541 gaaaggtcag tacgcatcca gccgttttgt ggatggctgc gtactgcgcc atccacatgg
     6601 aaaccatagg tttcattgtt ctgatagcga ttaaacagcg gcgtggaaag ggtacgcggc
     6661 aaggccgcgg caaagaataa agcatgttgg ttaaccgcgt tcagcacctc attttgcagg
     6721 gcggcgtata acgtgctgcg ggtgtcgacc tgttgattgt tcttaacttg cgcaccttgt
     6781 gcgccggtgg tgacgcgtcc atccacccat tcggcttgtt ccagttgttc gcgaaaccgg
     6841 gcgacgtcct gtggcgataa cacgccggga atgtggtaca tcatacaagt tctccaaaaa
     6901 gtggggcctg cgccccacat ctgaatcaga aatgcatatt ggctgtgagc aagaaggttc
     6961 ttggctcgcc cgggtgataa cggtagccgc tcttgttgat tgaggcgacg taatcggtat
     7021 caaacaggtt gtaaacgttt agctggaagt cgagattgcg attaacccga taccccagtt
     7081 tggcatcggc gacccagtaa ccttcggtaa acgctggcgt tcccaccgcg ccgtctgaac
     7141 ctttatgcat actgccgata tagcgtgcgc ccgcgccaac agagatatca tctgttgcct
     7201 gatactggct ccataaggtg aaggcgtgct ccggggtata cggcagcgat gaggaaccat
     7261 cctgggcaac atctttgccg tttttgatgg ttgctttttg ctgggtatag ccgccaatca
     7321 cctgccacgc gggcgtgata ttcccggcca cggatatctc atagccttcg acacgtttct
     7381 taccgtattg cgagtaagtt ccgtcatcat tttgctcaac ttcattttcg atatcagtac
     7441 ggaatagcgc ggcggtgagc aacaggcgtt tatccagaac ctgccatttg gtgccaatct
     7501 cgctggtgtt ggctttttgc ggtttaaaat cggtgcggtt ggcactgtta ccgctgccag
     7561 actgcgcaag ggcgaagttg ttgccgcccg gaggctgctg ggaaacggca tagttaatat
     7621 agacattacc gttttccgtc aggtgataca gcgccccggc tttccagttc accagattgc
     7681 ccgacttggc ggtgtcgacg gtggtgaccg gagaaccttt tgccacacca gctgggcagg
     7741 tgatggcacc gcgtccgctg ccgccgcagg cggtggcact gtcatattca gtatgataat
     7801 tatccagacg gatcccgccg ttcagctcaa aatcacgggt gatttgcagc gtatcgaagg
     7861 cgtaaattgc gaaggtatcc gtctgaccat tggcgtttgc gccgttgcgc gtcaggccgc
     7921 cgggatgaat gctgctgtca ggatgataaa tatttaccgc gggtaacgtc accggattaa
     7981 cgccgtagtt agtctgcgtt tcacgggtaa attccacgcc ggtactgaca tcatgaccga
     8041 tagaaccggt atagaacgtc gaggtcaggt tggtctggtt ggtgagaatt ttattactca
     8101 catctttggt attcgccgtg cgtgaccagg tccagctatt cacatcgctg gtgggctgag
     8161 taatattcga cgccccgccc ataatcgccg tcatcaggta atcctgcttt acgcgcgacc
     8221 aacgggtagt attgcgaatg gtggtgttat cgttgatgtc gtgctcaaaa cgcatggtgg
     8281 cggtgtcggt ggtcgaatcg tcgtaatcgg aatccgtgcc gtaaaagtta tgagtatcaa
     8341 cttttccgga atgattcaga gccgctgttc ccgcagatgg ggcagaataa cccggcaaac
     8401 cgatggtcgg aatgccgccg tctggcgtgt tgtgctgggt gacatgcaga taattaagat
     8461 acaaacgatt cgctgtacca aggccaaaag cgatagaagg ggcgacgccg taacgctcat
     8521 ttttgacttt gtcgcgtccg gcatcgtgcg ttttttcgcc catcacattc aggcgcaccg
     8581 cagtggtatc accaatgacc tgattgacgt ccagcgtgcc gcggcggaac caggcgctgc
     8641 caatactggc ggaggcgtca atgccggaat cattgcgcgg ctgcttgctg atcatattga
     8701 tcgagcccgt cggcgcgctg cgcccgtagt cggtgccgga aggcccttta atcacttcga
     8761 cctgctcagt attgaaggtg tcgcgcgaga cgctgccgat atcgcgaatg ccatcaatat
     8821 aaatactgtt agaggtatca gcaccgcgca tataaatggc gtcgccagtg gtggagttac
     8881 cgttctcacc cgcaaaaaac gcacccacgc cggggacgtt tttgagcgcg tcggtaaggt
     8941 tggttgcgcc ctgatcttta atcacttgtt cagaaatcac cgtcatcgtg cgggtagtat
     9001 ccgctaccgg acgcgagaat ttcggatcgg cagattgttg tggcgcataa agcgaaggcg
     9061 ttgatgcttc gacaaccagc gtgtcatccg cgttagtttg cccttcggcg gcgagtgcct
     9121 gagccacagg cgtgatgcca atacaaagac cggcaaagaa cgtgagcgaa tgaaattgtc
     9181 tggcagggaa attgcgattg ttttccattt tgcaggtgac ttttttttat atgttgaaat
     9241 tttcactgcc acgagaaagc ctctctgcgg atgcgatgcc actgttaatc tcgtgatagc
     9301 gttcgtgcaa gtgataataa ttttgataag cattatcact tggcgcgatt ttctatcacc
     9361 cattgcgcga aaaagaaata catttattta cattgcgttc atatttgatg ttttttgatt
     9421 acaaaacagg cgaaaactgc atccagaggc gaatgcagtt acgttggtgg ggttatttgt
     9481 agatggttgc agtaccgaac atctggtcat ttccgcctgc ggaattaatg acataacctt
     9541 tcgcgccttg ctcgcgcgct ttttctgcca gtttatcctc cagatcgctt aggtttgatg
     9601 cgccagttgc cgaaacggta ccggcgggcc gtagctgacc gctgtccagg ttactcatcg
     9661 gttgcgcggc ataagccgtg agagagattc cgctcaggac ggtggcaatc agtagtgtga
     9721 ggcacttttt cataatgaca tcctcataaa tacaacgggt agtgcctgat aagtgtaggt
     9781 cgcctgggcg atgaaaatga cgcaaaaaat tgacagagat gtgcttattt tttgaacgat
     9841 atatttttac aaaataagac aaatcaatga catgcataat gctgattttt atgatgaatt
     9901 gccataaacc atagccatgt taaggttttg gggaatgata aacgcagcca gatatttacc
     9961 ctgtcgcgtt atgcgggtat cttacgccgc tgcttaaagg agaatgctat gtccgcccag
    10021 aaaccggggt tgcatccgcg caaccgtcat cacagccgct acgatctcgc cacgctttgt
    10081 caggtcaatc ctgaactcag gcaattcctc acgcttacac ccgcagggga gcaaagcgta
    10141 gactttgcca atccgctggc ggtgaaggcg ctcaataagg cattgctggc ccatttttac
    10201 gccgtagcga actgggatat cccggacggt tttctctgcc cgccagtacc gggccgggcg
    10261 gattatattc atcaccttgc cgatttactg gcagaagcaa gcggaacaat tccagctaat
    10321 gccagcattc tggatatcgg cgttggtgcg aactgtattt atccgctgat tggcgtacat
    10381 gaatatggct ggcgttttac cggtagtgaa accagcagtc aggcgttaag tagtgcgcag
    10441 gcgatcatca gtgctaatcc ggggcttaac cgcgccattc gtctgcgtcg gcaaaaagag
    10501 agtggggcga tttttaacgg catcatccat aaaaacgagc aatacgacgc gaccttgtgt
    10561 aacccgccat tccacgattc cgccgctgcg gcacgggcag gtagtgagcg taaacgccgt
    10621 aacctggggc tgaacaaaga cgatgcactg aactttggcg gccagcaaca ggagttgtgg
    10681 tatgaaggtg gtgaagtcgc ctttatcaaa aagatgattg aagagagcaa aggcttcgcg
    10741 aagcaggtga tgtggtttac ctcgctggtt tctcgtggtg aaaacttacc gccgttgtat
    10801 cgtgctctga cggacgtggg cgcggtgaag gtggttaaaa aagagatggc ccaggggcaa
    10861 aagcagagtc gctttattgc ctggaccttt atgaacgacg agcagcgccg ccgttttgtt
    10921 aatcgccaac gttaaagcgt tggctccccc ggcggcagcg gttcagccgg ggccgcgccc
    10981 ggagcaggca gcacctgata agtctgcact ggcgcgcgaa cacctgccag gtcgaaatgc
    11041 tttttcacct ggctgtcgag ggcaaagcgg accgtccact gtttgagtgg cagcgtggtg
    11101 aacgaaacac gcagtgtaaa cgcggtattg cttaagccga caatcccggc aaagtttggt
    11161 tcaccaataa ccagcccgcg aatttcttcg ttttccatca attccgctac cgcatctttc
    11221 agtgcctgat tggctttatc agcatcttca tggcgatcaa catcgtagtt tgccaccacc
    11281 gaaccaatgc cgcggacgaa gttagcaaag gtggttatcg aagaccaagg aatgatgtga
    11341 tacgccccgg tatcctgacg cacgcccacg gagcgaatcg acatccgttc cacagtgccg
    11401 gtcaacggcc cgatagtaac caaatctcca gtgttcatgc cgttttcaaa ctgaataaat
    11461 accccggtga taatatcttt caccagcgtt tgcgaaccaa acgagatagc cagacctaat
    11521 gccccagcac ctgccagcaa tggcgcgata ttgacgccga tttccgacaa cacaatcatg
    11581 atggtgatgg tactgataat caccgccagc gcgttacgga acagcgtcag cagggtacgc
    11641 gtacgggcgc tgggtagcgg gcggccatga atatccgaag ccagccggtt ttcgatcaaa
    11701 ctggcgagca ctgtccagcc aaccgccgag aagaaaagaa tgagtgcgat gcggatcagg
    11761 atatctacgg ttttctgccc cgcgccgttt tgcagccagt tccagaaatc gaacaatccc
    11821 catgcgctca acagcaacat taccgccacg cagacggtca gaatacgcgc cgttttcagc
    11881 gccgccgaaa gccagccatt cagccgtttt tgtagctccg gatagttacg ctgagtatgt
    11941 ggcgagaggg tgatggtttt cgccagccag cgggaaaaca taccggaaac aaacgctgcg
    12001 ataccaataa tcgccaggct gcgcaccgtt gcacccatca taaatttcag gctgttgccc
    12061 ggatcgaaca acgaaaagaa aaacagcacg ataaaatagg cgcttgccag ccagtgccac
    12121 accagcgcaa aggcgcggat aaacaggctg aaaaaggcca gcgaatgctc cgcaaagttg
    12181 agcaaatgtt gggtaatctc ttttttattg cgaaagatca ggtacaacgc ccagacggtc
    12241 atgcacagca taatgatgac gttcgccagc gcacctatct gtacattcac ctgattagag
    12301 ataatcggca cggccacaat caggccataa cctatcaaac tgcttaacca gctcaggcga
    12361 cgactccagt aacgggcgct ctcgtcctga atcgtgaacg ggcgcagttc agccacgttt
    12421 gggcaaaaaa tcaggcgtag tacggctttg aaaaattcaa tcagggcaaa ggcgttgaga
    12481 aacaaacttt gttggaaagc gatggtgcga ctgcccgcat tcaggttgtc gcttaatacc
    12541 tggccgacaa acaatgtcag tgccagtaac agtaggtcga taataaacgc cccgataatc
    12601 atcgccggaa gctgcagcca gttgctgcgt tcgcgatttt tttgccgcgc ccactggccc
    12661 attttgcgat acagcggcag tgcgcacaga cgaatcagcc agtaaaaacc aaacactaat
    12721 accgctaaca ttgaaaaatg ggtcaacgca ttgctgaagg tttgtggatt aaacggctta
    12781 tgcggggagc cggtgatatt gcgataaagt tgtccgaaac gggcggaaag ggcttcacca
    12841 taatggcggc tgacttcggt gaccttttgc agcacggttt gctcttcaac cagcgtcggc
    12901 ggcacgatct tcgggaccgg ttccgcaggg ggcgtcgcgg cgacagtacg caactggtcg
    12961 atcaattctt tacgcgaggt gtcgttatcc agcacatccg ccagtgcgcc ataggccgct
    13021 tttttttgtt cgatatccgg ttccggggcc ggttcagtcg ttgagtccgt tgtcgttgtg
    13081 gttgtaacgc cgggtatgga taccgcgtgg gcaggtgcgc ccagcaggca gaagaggatg
    13141 aacaggatcc accgcatgac tcctccagtg agaaaacaga gcaaaaagat aagtatagat
    13201 gctgggggag gggcgatttt aaatgagagg aatctggtgt gcctcccttt cgggtgaaag
    13261 ggaggcagag aagttaagag acgtgctgca aaaattcctg caagcgctgg ctcggcgggt
    13321 tcttgatcaa tacctgcgga tcgccatctt ccgcaatccg gcctttgtcg ataaagatca
    13381 gccgcgaagc tactttctcg gcaaaaccga tttcgtgggt cacgatcacc atcgtcatcc
    13441 cttcttcagc cagatcctgc ataaccttca gcacttcatg gcgcagttcc gggtcaagag
    13501 cggaagtcgg ttcatcaaac agcatcattt tcggcttcac cgccagcgcg cgggcaatcg
    13561 ccacacgctg ctgttgacca ccagaaagtt cggaagggta gtgatgtgca cgttctgcca
    13621 gaccgacttt cgccagcagc tcacgcgcca gtttttccgc ctcttctttg ttcgcgccac
    13681 gcacgcgtag cggaccaaac atgacgtttt ccagcgccgt cagatgcggg aagaggtaaa
    13741 actgctggaa caccataccc gcttcctggc gaatcaggcg ctcgtcaact ttcggatcgt
    13801 taaccttcag gccatcgaca atcagatcgc cggaggtgat ttcttccagt ttgttgatgc
    13861 agcgcagcag ggtcgattta ccggaaccgg acggcccgat aatcaccacg acttcgccct
    13921 gagcaatgtt caaatcgata ttgtgcagca cctgggttgg accaaaatgc ttggagacgt
    13981 ttttaaattc aatcacagga ttttcatcct tctttccaga cgacgcagaa taaagctcag
    14041 caccagggta ataatcagat agaacaccgc cacggcgctc cagatctcaa gggcgcggaa
    14101 gttaccggca ataatttctt gcccctgacg ggtcagttcc gccacgccga taacaataaa
    14161 cagcgaggtg tctttaatgc tgatgatcca ctggttaccc agcggcggca gcatacgacg
    14221 cagtgccagc ggtaaaatga cgtagcgaat ggtttcccaa cgtgaaagac cgagcgccag
    14281 tcctgcttca cgaaaacctt tgtgaataga cagcaccgca ccacgcgtga tttccgcaat
    14341 gtacgcgccg gagttgatca tgatggtgac caccgccgca gtaaatgggt cgatgcgtaa
    14401 gtcgttaaac gccatcggca gggcgaaata gataaacatc acctggacga cgataggtgt
    14461 gccgcggatc acttcaataa agaccagcgc gacgtggttg gctatccaac caccgaaggt
    14521 gcgtgcaaaa cctgccagca atccgattac cagaccgcct gccagaccga ggaccgaaat
    14581 ccacagggtc attttggcac cttcaatcag aagcggaatg gcaggccaga tggcactcca
    14641 gtcaaactgc atatgttgtt cctgttaccg tggtgtaaat aatcaaattc agggtaatta
    14701 atggccccgg agcgggtttg cgcccctcac cctaatcctc tccccagagg ggagagggaa
    14761 ctgccagtgc gttttacagg tgtagcgtta ttatttcggt tcagtaccga accatttttt
    14821 gtagatttcg ttgtaagttc cgttctcgcg cagggttttc aacgcgccgt tgactttgtc
    14881 acgcagctcg tcgctacctt tcgggaacgc aataccgtat tgctgcgctt ccagagagtc
    14941 acctaccgct ttgaactgac cgttaccggc ggttttgatg aagtacagaa tgtttggcgt
    15001 atcgtgcaga acggcgtctg cgcggttggt gcccagttcc atataggcgt tatcgatgtt
    15061 cgggaactga cgcagatctt tagttttgat gtttgctttc gcgtaatcaa cggagccagt
    15121 accgctcttc acagcaacca ctttcccgtc gagatctttc acgcttttca catcgttatt
    15181 gttagctttc accatcacta acaggccgct tttgtagtag ccgtcagaga aatcgatcgc
    15241 ttttttacgc tcgtcggtga tggtaatgcc cgccagcgcc agatcgacgt ttttggtttg
    15301 cagtgccgga atgatcccac tgaaatccat cggcttcagt tcgtaatcca gcttcagctc
    15361 tttagcgatg gcagcccaca gatcaacgtc aaagcccaca tatttatcgc cctgtttaaa
    15421 ttcaaacgga acgaaggcgg tatccgtcgc gacaactaat tttttatccg cggcatgaga
    15481 agaaaccgca aaagccaggg tcagtgcagc cagtgaaact tttaatacag acttcatagc
    15541 atttcctttt ttctatccac gggacgatcc cctgcgtgac attcacatat atgaaaaaat
    15601 cgtgccagtt ttgcaactca ttgatttttc gagatgcggc aaaaacgtga tgcacgattt
    15661 atagggcaat accctgaaga tgcaccattc tggggcacca atctggtgcg ctaaaattgt
    15721 gcactcaaca cagtgattat ttagcgtaaa acctgatgaa aaaacaatct ttatgtaacg
    15781 attgtgtgat gatgtggata caaaaaattt acttaatcag ctggagatag cagatggatg
    15841 cactaaataa gtgcgttgag gtgggctgca aaacaaaacg gcctcctgtc aggaagccgc
    15901 ttttatcggg tactaaagtt ctgcaccatc agcgatggat ttattcgatg ttagactcga
    15961 taaaccacag gaatttatcc aggtcgcgag acgcggcggt caggatatct gcggtgtcgt
    16021 catctttcgc ttcgccaatc gctttgcgta cgtcattagc gacgattgcg taacggtcag
    16081 ccagttcttt caggtgatcc tgaacgttgt ggatgtccag cgggtaactt ttcagcgggg
    16141 ttttgctgtt gataacttga gtggtcccca gagctacacc gcccagctgc actgcacgtt
    16201 ctgccatggt atccagatga tcgatcagtg cggtgcggaa gccatccagc atttcatgta
    16261 cggcaatgaa gttagcgccg cgcatgttcc agtgcgcttg tttggtaatc aaagaaagat
    16321 caataaactg gataacctgg cgattcagca actctactgt tgcttttttc tcgctgtcgg
    16381 agacatcgtt gcgggtataa agcagattgg tcgcttttga tttaactaat ttagcggtac
    16441 tcataatttc atatcctctt gatgttatgt cccagtaatt aacgagacta agtatagcac
    16501 cggctatgtg ttccgctatt ctggctgttc ctatcacact aatagtggta acaagcgtga
    16561 aaaacaaaac taataaagat tcaatgagtt agatatattg ataagaacaa ttcttattta
    16621 tctgctcgcc aggaattttc ggaataaata tgcttttgca taattcgata tataaagacg
    16681 gtgtagagga aaagtagcga gaaattctgc atggttatgc ataaccatgc agaaatgtaa
    16741 atttaattaa tgtctaattc ttttattttg ctctctttgc gtactgtcag cgtagacccc
    16801 attgaagcgg cgatgatagc gccgagcgcc agcagctgta tgggtgtcag tgtttctccg
    16861 aggaaaatca tcccggaaac ggcagccagc gccggttcca tgctcatcag cgtaccaaat
    16921 gtccgtgttg gcaaacgggt gagggcaatc atttccagcg aataaggcag agcggtcgag
    16981 agaatagcga cagccagacc caatggaata accgaccagt gccagagtgc ttcaccagcc
    17041 tgaagcgctc caattggcac gaaaattaac gctgcaatca acgaaccaat tgccaccgtc
    17101 gcagggccat gttccgctcc tgcgcgttgc ccacttaaaa tgtaaatagc ccaacaagcc
    17161 ccggccccca gtgccagcgc acagccggtt aaatcgacat gggaaacgtc ttgccccagc
    17221 ggtagcagga accacagacc aagaaccgcc agcacaaccc agacgaaatc taccgggcga
    17281 cgagaagaga acagcgccac cgccagtggt ccggtgaact ccagcgccac cgcaataccc
    17341 agcggtactg tctgaataga aagataaaaa agataattca tcccacccag cgaaacgccg
    17401 taaaacaaca gcggtaaccg ttgctctttg gcaaagcgca gtcgccatgg cttaaagaac
    17461 gcgatgagga tcagcgttcc taatgccaga cgcagcgcag tgacacccgg tgcgcccacc
    17521 agaggaaaaa gtgacttagc taacgaggct ccaccctgaa tagacgccat ggcaacgagc
    17581 aatatgatta ttggtaacca gaccggcatt ttacgtaatg aaccaggcat cctttctccc
    17641 acaaatatct agacttaagt aaagcgtgga gtgtactgga tatacccaat gctggttgag
    17701 catttgttga aaaaattttc ccccgttttg actaaaatgc gccaggattg atggaatcat
    17761 tagtctggtg attaggaata atctggatga atgacaggga aaacatgcgt aatacttacg
    17821 cagttctctg aaaaagtgat ttaaatttag atggatagcg gtgtatggaa acgttctgtt
    17881 acatgaaatg gcccgttaga catcacaaat cgcgaagagt ttcccattaa tttttgatat
    17941 atttaaaact taggacttat ttgaatcaca tttgaggtgg ttatgaaaaa aattgcatgt
    18001 ctttcagcac tggccgcagt tctggctttc accgcaggta cttccgtagc tgcgacttct
    18061 accgtaactg gcggctacgc acagagcgac gctcagggcc aaatgaacaa aatgggcggt
    18121 ttcaacctga aataccgcta tgaagaagac aacagcccgc tgggtgtgat cggttctttc
    18181 acttacaccg agaaaagccg tactgcaagc tctggtgact acaacaaaaa ccagtactac
    18241 ggcatcactg ctggtccggc ttaccgcatt aacgactggg caagcatcta cggtgtagtg
    18301 ggtgtgggtt atggtaaatt ccagaccact gaatacccga cctacaaaca cgacaccagc
    18361 gactacggtt tctcctacgg tgctggtctg cagttcaacc cgatggaaaa cgttgctctg
    18421 gacttctctt acgagcagag ccgtattcgt agcgttgacg taggcacctg gattgccggt
    18481 gttggttacc gcttctaatc actttggtga tataaaaaat ccgcctctcg gggcggattt
    18541 ttgtttttaa ggtttcgggt cgaaaatatc ggttccgaga tggttgtaat ccaccttctg
    18601 taactggaag ttggtgatgt atatcggccc ggctttcttc tcagatataa acgggtattt
    18661 gatatttatt tccttcgctt taattcccgt ccactgcgag aaaaagccta agaagtcatt
    18721 ggctgagcgg cgggctttaa tcacccgatg cgctttatcg tcgctggaaa tgaccataaa
    18781 aggcacctgg aaattttgct gatatttatc atcatgggca aggtattgca cgtctttgcc
    18841 gcgctcttta aaggccagac cgtggtcaga aaagtaaacc agagagaagc tgctgccgct
    18901 gttgcgtaac tgatcgtaca gcttgcgcag taaatcgtcc gtttgcgtca tggtatagag
    18961 atagcacgac gtttctttcg attgcacaaa ggtttcgtat ttgccttgtg tcctgtcgca
    19021 ggcctgcgga tgcgagccca tcagatgcag aacaatcagc tgcggttgcg agtgctcttg
    19081 cgccagcact tgagcggtca tatccagtaa cgcttcgtct ttcgtgtttt tatctgcttc
    19141 aaaattacct tctttcagga agtacacttc atctgcacgt ttggcgatgc tggcgatagc
    19201 agtatcgtat tcgccgattt ggccctggtt ggaaaaccac caggtctgga agcccgcgcg
    19261 atttgccagg gtgacaaagt tatcctgaaa ctgtggtttg ccatcgacta cgcgattgag
    19321 cgttaagcca agcgatttct gcgtggagcc actggcggca atgtagtcag caaatatcaa
    19381 accgttaacg ctgctggcaa acggggtatt gtcccagtga ccgccaaagg caccgagggc
    19441 atcgcgacgc gcgctttcac cgatcaccac cacataggtc tgatacttcg gcttaacggc
    19501 agtgaccgtc caggtatctt tcattccgga aagttttgcc atccgttctt gctcttcaat
    19561 cacctcgtta ttattgacga tgacatcctt agcaaaacga aataccggat agccagtatc
    19621 tttcagttta aatacgccac cccaggccag gttttgcacg ggagcaacaa aaaatgtcgc
    19681 cacactgaat accagacaca ggctgtcgaa ggtattccag cgcgcttttt cattctcttt
    19741 ttttcgcctg attgctatta cgccgagcgc aaaaataaat aagccgacca ggtagctgta
    19801 ccacggaaaa atcgtcagga tttcggtcga ctcttccata ttggtggagt gcaatgccag
    19861 caatgtattg aaattaggcg cgccgtaggc ctgagcaaaa gggaaataac aagccgccac
    19921 cagcgaactg acaccgacca gaactttttg tacgcgaggc aatgttcgcc atagcaaaag
    19981 caaaatagcc gtaaacgcag cggtgtagag caaactgaag gggtaaccta agccgaggtt
    20041 aattaatagc gactgtaaaa agtagaacgc agtccacggg ctaaataccc ggctacgggt
    20101 aacaagcgat tctttgaggg ttaaattcat atgccactat cgtaacaaac gccatgtgct
    20161 taccctggcg tcaagggtca atacctgcaa gagtgcgtga agagtggaaa ggggtccgca
    20221 tccgcgagcc gcaatatacg cagggctgca agaagataga gcgagccgcc gttcaggtca
    20281 actactgacg aacaaatctt ttgcgaaggg gattgcaaat ccgacaaaaa gtggggtttt
    20341 tcagaccaaa acgcgttatg cctgcatgaa agcaggagtg aagcggcgta ccgcgacgcc
    20401 gctatttatc ggaaggttta tcttgctgcg gtttgttgtt gaccatatcg cacaacatag
    20461 agagcagcat taaccgtact ttaaagggag aatgactaaa cacgcgcata cacctcttga
    20521 actcattcat aagacctcct gacttgctaa tcccgtcgat ccttgaggga tgattgcatt
    20581 acatacagat atagcacagg ctatattata tagctattgc taaaacgtta attttttgta
    20641 ccttcgcaac tctggtttac aatgtgcgca cgaaatgaga acgctatgta tgcgtcacca
    20701 taatgaggaa gcacaatgag tcgtcgcgca ggtacgccaa cagcaaaaaa agtgacgcag
    20761 ttagtgaacg tggaagagca cgttgaaggg ttccgccagg tcagagaggc gcatcggcgc
    20821 gagcttattg atgattacgt tgagctgatt tctgacttga tcagggaagt gggggaagct
    20881 cgtcaggtgg acatggctgc tcgtctggga gtttcgcaac cgacggtggc taaaatgctt
    20941 aagcggctgg caaccatggg gctgattgaa atgatcccct ggcgtggcgt gtttttaacg
    21001 gcagaaggag agaagctggc gcaggaaagc cgcgagcgac atcagatagt cgaaaatttc
    21061 ttgttggtgt tgggcgtcag tccggaaatc gcccgtcgcg acgcggaagg catggagcac
    21121 catgttagtg aagagacgct cgacgctttt cgtttgttta cccagaaaca cggtgccaaa
    21181 tgagcctgcc ttttttacgc acgctgcaag gcgatcgttt ttttcagtta ttaattcttg
    21241 ttggtatcgg attaagcttt ttcgtgccct ttgcaccgaa atcctggcct gctgctatcg
    21301 actggcacac catcatcacc ttaagcggcc tgatgctgct gaccaaaggt gtggagttaa
    21361 gcggttattt tgatgtgctg gggcgcaaaa tggtgcgccg ctttgctacg gagcgtcggc
    21421 tggcgatgtt tatggtgctg gcggcggcgc tgctttctac ctttctgacc aacgatgtcg
    21481 cgctgtttat tgttgttccg ctgactatca cgctaaaaag actgtgtgag atcccggtta
    21541 atcggctgat tatttttgag gcgctggcag tcaacgctgg ttcgctactg acgccaattg
    21601 gcaacccgca aaatattctt atctggggac gttctggtct ttcgtttgcc ggatttattg
    21661 cccaaatggc accgctggct ggcgcaatga tgctgacgct cctgctcctg tgctggtgtt
    21721 gtttccctgg aaaggcgctg caataccata cgggggtgca aacaccggag tggaaaccgc
    21781 ggctggtgtg gagttgtctg gggctgtata tcgtctttct gacggcgctg gagttcaaac
    21841 aagagctgtg gggactggtg attgtggcgg caggctttgc gctgctggca cgtcgcgtgg
    21901 tgctcagtgt ggactggacg ctgctgctgg tgtttatggc gatgtttatc gacgtccatt
    21961 tactgaccca gcttccagcg ttgcaaggcg tgttgggtaa cgtgagtcat ctatctgaac
    22021 ccgggttatg gttaacggca atcggtttat cgcaggtgat cagtaacgtg ccgagtacca
    22081 tattgttgct gaactatgtg ccgccgtctt tattactggt atgggcggta aacgtaggtg
    22141 gctttgggtt attacccgga tcgctggcaa atttgattgc gctacgtatg gcgaacgatc
    22201 gccgcatctg gtggcgtttc catctctatt caataccgat gctgttgtgg gcggcgttgg
    22261 tgggatatgt tttgttagtt atactcccgg ccaactaggg tctggcaaat aaaaaaggcg
    22321 gattatgagt ccgccttttg ctttaagttt tgaagattaa ttcagacgaa ccggcatccc
    22381 ggagcggttt ttaatcgctt catcaagaac aacctggtca acatctggct gaccggtcac
    22441 tgtttgcacg ctcttcgtca gggtaattgg cacaatttcc tgaccttcaa actgggcttc
    22501 ggtggtagac agcgggttat ggacttcaat ataacggctg ccgtctggct cggtggtcgc
    22561 ttttaccggc tcatcaataa actgtacgcg ggtaccgacc ggtactttct cgaacaggaa
    22621 tttgatgtct tcgttacgca gacgcacaca accatgactt acacgcaggc cgataccgaa
    22681 gttggcgttg gtgccatgga tagcgtacag gcgaccgatg tagagtgcat acagccccat
    22741 cgggttatcc ggacctgccg gaacgacagc cggaagcggt tcgcccgcag cgcggtactc
    22801 tgcgtgcatt ttggcggtcg gcgtccaggt cgggcctgct ttcttacgct caactttggt
    22861 ggtccagttg ataggcgtat ctttgcctaa ctgaccaatg ccgatcggca gcacgataac
    22921 ggtgttggtc cctttcggat agtaatacag acgcatctct gcactgttaa tgacgatgcc
    22981 ttcatgaacg gtatccggca ggatcagctg ctgcggaatg ttcagtacag taccgccttt
    23041 cggcaggaag gtatccacac ccgggttcgc ttccatcata ttggaaagcc ccatctggta
    23101 ctccgcggca aaatactcca gcggctgagt gttaccttca ggaatggtga tcacctgatt
    23161 ctgaccaacc aggcgactcc cgtcggttgg cagaggataa gttaccgcag aggcggtact
    23221 gcaaaagccg acaacagcga aggccgctgc gaataatgtt ttcaatttca tattcatgtt
    23281 aagcgagatt ttgtgcctgg caggccattg ggttgagaat attagagtat tggaagcgca
    23341 ttataagttc attccagctc acagtgaaat cagatgtgta cgaaatcaca ttttttgcct
    23401 ttggcttgag tgtagacctt aagcgaggag caggatcttc tttcagactt atggcataat
    23461 gcgcggtttg tcatatctct tttcaggata cgcctgtgtt agtttccagt aacgtcacca
    23521 tgcagttcgg cagtaagccg ttgtttgaaa acatttccgt caaatttggc ggcggcaacc
    23581 gttacggcct gattggcgcg aacggtagtg gtaaatccac ctttatgaag atcctcggcg
    23641 gcgacttaga gccgactttg ggtaacgttt ccctcgatcc caacgagcgc attggtaagc
    23701 tgcgtcagga tcagtttgcc tttgaagagt tcactgtgct ggatacggtg atcatggggc
    23761 ataaagagtt gtgggaagtg aagcaggagc gtgaccgcat ctatgctttg ccggaaatga
    23821 gtgaggaaga cggctataaa gtggccgatc tggaagttaa atacggcgaa atggacggtt
    23881 actctgcgga agctcgcgcc ggtgaattgt tgcttggcgt gggaattcca gtggaacagc
    23941 actacggccc gatgagtgaa gttgctcctg gctggaagct gcgtgtgctt ctggcgcagg
    24001 cgctgtttgc tgatccggat attctcctgc tcgacgaacc gaccaataac ctcgacatcg
    24061 acaccattcg ctggctggaa caggtgctga acgagcgtga cagcaccatg atcatcatct
    24121 cgcacgaccg tcacttcctt aacatggttt gtacccacat ggcggatctg gattacggcg
    24181 agctgcgcgt ttatccgggc aactacgatg agtacatgac ggcggcgacc caggcgcgtg
    24241 aacgtctgct ggctgataac gccaagaaga aagcgcagat tgctgagctg caatctttcg
    24301 ttagccgctt tagcgccaac gcctcgaaat ctcgccaggc aacttcgcgc gcgcgccaga
    24361 ttgataaaat caaactggaa gaggtgaaag cctccagccg tcagaacccg ttcatccgtt
    24421 ttgaacagga taagaaactg ttccgtaacg cgctggaagt ggaaggtctg accaaagggt
    24481 ttgataacgg tccactgttt aaaaatctca acttgctgct ggaggtcggt gaaaaacttg
    24541 cggtactggg taccaacggc gtcggtaaat caacgctgct gaaaacgctg gtgggcgatc
    24601 tgcaaccgga cagcggcacc gtaaaatggt ctgaaaacgc gcgcattggg tactatgctc
    24661 aggaccacga atatgagttt gaaaatgatc tgaccgtgtt cgaatggatg agccagtgga
    24721 agcaggaagg cgatgacgag caggcggtac gcagcattct cggtcgtttg ctgttcagcc
    24781 aggacgacat caaaaagcca gctaaagtgc tttccggtgg ggaaaaaggg cggatgctgt
    24841 ttggtaagtt aatgatgcag aagccgaaca ttctgatcat ggacgaaccg accaaccacc
    24901 tggatatgga atccatcgag tcactgaaca tggcgctgga actgtatcag ggcacgctga
    24961 tctttgtttc acacgaccgt gagttcgtaa gctccctggc gacccgcatt ctggaaatca
    25021 ccccggaacg cgtgatcgac tttagcggta attacgaaga ttacctgcgt agtaaaggga
    25081 tcgagtaagt gattatccgt caccccgata ctgtcggggt gacggcggct gatgacaaat
    25141 gcaaaatagc ctgatg
//
LOCUS       DAZGSP010000072        24948 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZGSP010000072 DAZGSP010000000
VERSION     DAZGSP010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.072"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..759
                     /locus_tag="ACHK74_003276"
     CDS             <1..759
                     /locus_tag="ACHK74_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFP0222042.1"
                     /translation="GNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDI
                     VWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAIN
                     FSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEG
                     IPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMD
                     FAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            915..2450
                     /gene="gadC"
                     /locus_tag="ACHK74_003277"
     CDS             915..2450
                     /gene="gadC"
                     /locus_tag="ACHK74_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFP0222043.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2581..3900
                     /locus_tag="ACHK74_003278"
     CDS             2581..3900
                     /locus_tag="ACHK74_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFP0222044.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPGWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4272..5654
                     /gene="dosC"
                     /locus_tag="ACHK74_003279"
     CDS             4272..5654
                     /gene="dosC"
                     /locus_tag="ACHK74_003279"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFP0222045.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5679..7961
                     /gene="dosP"
                     /locus_tag="ACHK74_003280"
                     /gene_synonym="pdeO"
     CDS             5679..7961
                     /gene="dosP"
                     /locus_tag="ACHK74_003280"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFP0222046.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELLLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDNKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQ
                     LTVEITESMMMEHDTEIFKRIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDK
                     SFVDRCLTEKRILALLEAITSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFS
                     RPLPAEEIPGWMSSVLPLKI"
     gene            8219..8800
                     /gene="ddpX"
                     /locus_tag="ACHK74_003281"
     CDS             8219..8800
                     /gene="ddpX"
                     /locus_tag="ACHK74_003281"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFP0222047.1"
                     /translation="MSDTTELVDLAVIFPDLESELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            8814..10363
                     /locus_tag="ACHK74_003282"
                     /pseudo
     CDS             8814..10363
                     /locus_tag="ACHK74_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            10365..11387
                     /locus_tag="ACHK74_003283"
     CDS             10365..11387
                     /locus_tag="ACHK74_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0222048.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGNPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11384..12280
                     /gene="ddpC"
                     /locus_tag="ACHK74_003284"
     CDS             11384..12280
                     /gene="ddpC"
                     /locus_tag="ACHK74_003284"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFP0222049.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12277..13263
                     /locus_tag="ACHK74_003285"
     CDS             12277..13263
                     /locus_tag="ACHK74_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0222050.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTLQLQVLRLLKHKARASGTAVLFISHDMV
                     VVSQLCDSVYVMYAGSVIESGVTADIIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            13256..14182
                     /locus_tag="ACHK74_003286"
     CDS             13256..14182
                     /locus_tag="ACHK74_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP0222051.1"
                     /translation="MSNTLLTLCDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEQTSALDISVQAQILNLLVTLQVNRGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(14238..14669)
                     /gene="osmC"
                     /locus_tag="ACHK74_003287"
     CDS             complement(14238..14669)
                     /gene="osmC"
                     /locus_tag="ACHK74_003287"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFP0222052.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            15015..15230
                     /gene="bdm"
                     /locus_tag="ACHK74_003288"
     CDS             15015..15230
                     /gene="bdm"
                     /locus_tag="ACHK74_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFP0222053.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15332..15469
                     /gene="sra"
                     /locus_tag="ACHK74_003289"
     CDS             15332..15469
                     /gene="sra"
                     /locus_tag="ACHK74_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFP0222054.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15626..17323
                     /gene="maeA"
                     /locus_tag="ACHK74_003290"
     CDS             15626..17323
                     /gene="maeA"
                     /locus_tag="ACHK74_003290"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP0222055.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17457..18467
                     /gene="adhP"
                     /locus_tag="ACHK74_003291"
     CDS             17457..18467
                     /gene="adhP"
                     /locus_tag="ACHK74_003291"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFP0222056.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            18613..18897
                     /locus_tag="ACHK74_003292"
     CDS             18613..18897
                     /locus_tag="ACHK74_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP0222057.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(19150..19803)
                     /gene="fdnI"
                     /locus_tag="ACHK74_003293"
     CDS             complement(19150..19803)
                     /gene="fdnI"
                     /locus_tag="ACHK74_003293"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFP0222058.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19796..20680)
                     /gene="fdnH"
                     /locus_tag="ACHK74_003294"
     CDS             complement(19796..20680)
                     /gene="fdnH"
                     /locus_tag="ACHK74_003294"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFP0222059.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20693..23740)
                     /gene="fdnG"
                     /locus_tag="ACHK74_003295"
     CDS             complement(20693..23740)
                     /gene="fdnG"
                     /locus_tag="ACHK74_003295"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(23153..23155),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP0222060.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            23972..24853
                     /gene="yddG"
                     /locus_tag="ACHK74_003296"
     CDS             23972..24853
                     /gene="yddG"
                     /locus_tag="ACHK74_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFP0222061.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAVWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         6099 a         6357 c         6306 g         6186 t
ORIGIN      
        1 ggtaactatg agttcccaca accgctgcac gatgcgctgg ataaattcca ggccgatacc
       61 ggtatcgaca tcgacatgca catcgacgct gccagcggtg gcttcctggc accgttcgtc
      121 gccccggata tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag tgcttcaggc
      181 cataaattcg gtctggctcc gctgggctgc ggctgggtta tctggcgtga cgaagaagcg
      241 ctgccgcagg aactggtgtt caacgttgac tacctgggtg gtcaaattgg tactttcgcc
      301 atcaacttct cccgcccggc gggtcaggta attgcacagt actatgaatt cctgcgcctc
      361 ggtcgtgaag gctacaccaa agtacagaac gcctcttacc aggttgccgc ttatctggcg
      421 gatgaaatcg ccaaactggg gccgtatgag ttcatctgta cgggtcgccc ggacgaaggc
      481 atcccggcgg tttgcttcaa actgaaagat ggtgaagatc cgggatacac cctgtacgac
      541 ctctctgaac gtctgcgtct gcgcggttgg caggttccgg ccttcactct cggcggtgaa
      601 gccaccgaca tcgtggtgat gcgcattatg tgtcgtcgcg gcttcgaaat ggactttgct
      661 gaactgttgc tggaagacta caaagcctcc ctgaaatatc tcagcgatca cccgaaactg
      721 cagggtattg cccagcagaa cagctttaaa catacctgat aacgtttaac ggtaacggtg
      781 tcccgaaacg aacccgtttc gggacaattt ccaaagtctg ttcactggca ttagcaacgg
      841 aaaatattgt tctgaatacg cttcagaaca aaacaggtgc ggttccgaca ggaataccgt
      901 tttaggggga taatatggct acatcagtac agacaggtaa agctaagcag ctcacattac
      961 tcggattctt tgccataacg gcatcgatgg taatggctgt ttatgaatac cctaccttcg
     1021 caacatcggg cttttcatta gtcttcttcc tgctattagg cgggatttta tggtttattc
     1081 ccgtgggact ttgtgctgcg gaaatggcca ccgtcgacgg ctgggaagaa ggtggtgtct
     1141 tcgcctgggt atcaaatact ctggggccga gatggggatt tgcagcgatc tcatttggct
     1201 atctgcaaat cgccattggt tttattccga tgctctattt cgtgttaggg gcactctcct
     1261 acatcctgaa atggccagcg ctgaatgaag accccattac caaaactatt gcagcactca
     1321 tcattctttg ggcgctggca ttaacgcagt ttggtggcac gaaatacacg gcgcgaattg
     1381 ctaaagttgg cttcttcgcc ggtatcctgt tacctgcatt tattttgatc gcattagcgg
     1441 ctatttatct gcactccggt gcccccgttg ctatcgaaat ggattcgaag accttcttcc
     1501 ctgacttctc taaagtgggc accctggtag tatttgttgc cttcattttg agttatatgg
     1561 gcgtagaagc atccgcaacc cacgtcaatg aaatgagcaa cccagggcgc gactatccgt
     1621 tggctatgtt actgctgatg gtggcggcaa tctgcttaag ctctgttggt ggtttgtcta
     1681 ttgcgatggt cattccgggt aatgaaatca acctctccgc aggggtaatg caaaccttta
     1741 ccgttctgat gtcccatgtg gcaccagaaa ttgagtggac ggttcgcgtg atctccgcac
     1801 tgctgttgct gggtgttctg gcggaaatcg cctcctggat tgttggtcct tctcgcggga
     1861 tgtatgtcac agcgcagaaa aacctgctgc cagcggcatt cgctaaaatg aacaaaaatg
     1921 gcgtaccggt aacgctggtc atttcgcagc tggtgattac gtctatcgcg ttgatcatcc
     1981 tcaccaatac cggtggcggt aacaacatgt ccttcctgat cgcactggcg ctgacggtgg
     2041 tgatttatct gtgtgcttat ttcatgctgt ttattggcta cattgtgttg gttcttaaac
     2101 atcctgactt aaaacgcaca tttaatatcc ctggtggtaa aggggtgaaa ctggtcgtgg
     2161 caattgtcgg tctgctgact tcaattatgg cgtttattgt ttccttcctg ccgccggata
     2221 acatccaggg tgattctacc gatatgtatg ttgaattact ggttgttagt ttcctggtgg
     2281 tacttgccct gccctttatt ctctatgctg ttcatgatcg taaaggcaaa gcaaataccg
     2341 gcgtcactct ggagccaatc aacagtcaga acgcaccaaa aggtcacttc ttcctgcacc
     2401 cgcgtgcacg ttcaccacac tatattgtga tgaatgacaa gaaacactaa ccatgtctga
     2461 atggttataa gacaagggag cgataattca tcgctccctt tttcgtgctt gctgtcgtat
     2521 tgacttaacg gggataagta cgagaatgag cgcacatctg tttaccggaa accagcacat
     2581 atggatatct gctcccgaaa cgagaaatta gcgattagaa gaccggcgat actggttgca
     2641 ctggcacttt tactgtgtag ttgtaaaagc acgcctccag agtccatggt gacaccacca
     2701 gcgggttcaa agccaccagc cacgacgcaa caatcgtcac aaccgatgcg tggcatctgg
     2761 ctggccacgg tgtctcgcct cgactggcca ccggtttcct cggttaacat tagtaacccc
     2821 accagccggg cccgtgtaca acaacaggcg atgatcgaca aactggatca tctgcaacgt
     2881 ctcggtataa acacggtctt tttccaggtc aagccggacg gtaccgccct gtggccatcg
     2941 aaaattttgc cgtggtccga tcttatgacc ggtaagattg gtgaaaatcc gggttacgat
     3001 ccgctgcaat ttatgctcga cgaagcccac aagcgtggga tgaaagtaca cgcctggttt
     3061 aacccctatc gcgtatcggt taatacgaag cccggtacta tcagggaact gaatagcact
     3121 ctgtctcaac aaccggcgag cgtctatgtg caacaccgcg actggatcag aacgtctggc
     3181 gatcgctttg tcctcgaccc gggcatccct gaggttcagg actggatcac atcaatagtc
     3241 gcagaagtgg tttcccgcta tcccgtagat ggcgtgcagt ttgacgacta tttctatacg
     3301 gagtcaccgg gttcacggct aaatgataac gaaacgtacc gtaaatacgg aggcgcattt
     3361 gcgtcaaaag cagactggcg gcgcaacaat actcagcagt taattgcaaa ggtatcgcac
     3421 accattaaaa gcattaagcc gggagtcgaa tttggtgtta gcccggcagg cgtgtggcgt
     3481 aaccgatcac acgatccgct cggttccgat acccgaggcg cggcagccta tgacgaatcc
     3541 tacgctgaca cccgtcgatg ggtggaacaa ggattgctgg attacattgc tccccaaatt
     3601 tactggccgt tctcacggag tgccgcgcgt tatgacgtgt tggcaaaatg gtgggcggat
     3661 gtcgttaaac cgaccaggac ccgcctgtat atcggtatcg ccttctataa agtgggtgaa
     3721 ccttcaaaga tagagccagg ctggatgatt aacggcggcg taccggaact gaaaaagcag
     3781 ctcgatctta acgatgcggt accagaaatt agcggcacca tcttgttccg tgaggactat
     3841 ctgaataaac cgcagactca acaagcggtc agctatctgc aaagtcgttg gggcagttaa
     3901 actcccattc ttactttgta acaagccggt gctttaccca ccggcttgtt accctcttga
     3961 actatcccac tactaatcat gcttactaaa gtcaaattaa ccacgcaatt aaccacactt
     4021 agttaaacag ccataaatcc cctaataaaa atgtatgaat atgaaaaaac atacgatatt
     4081 cgctgtcacc gaaaaataat tacgatgggc tatactcttc tgaagaacag tagcgtagat
     4141 taaaactgtc ctaagtcgct tatttctttt cagtatatct tcatatttca ggagaatatg
     4201 gtgcagttag tcgatctcgc acgttgcgtt ttgattccgt ctgatttccc agccttataa
     4261 gggtgtgaat tatggagatg tattttaaaa gaatgaaaga tgagtggacc ggacttgtcg
     4321 aacaggcaga tcctctcatt cgtgctaaag ccgcagaaat tgcccttgcg catgctcatt
     4381 atctgagtat cgagttttat cgaattgtcc gcatcgaccc gcatgccgaa gaattcttga
     4441 gtaatgaaca agttgagcgg cagttgaaaa gtgcgatgga acgctggatt attaacgtgc
     4501 tttctgccca ggttgacgat gtcgaaaggc taatacaaat ccagcatacc gtcgcggaag
     4561 tgcatgcccg cattggaatt ccggtagaaa ttgtcgagat ggggtttcgg gtgctgaaaa
     4621 aaatcctcta tccggtaatc ttctcttcgg attattctgc cgcagaaaaa cttcaggtct
     4681 accatttctc gattaacagt attgatatcg ctatggaagt gatgacccgc gcgtttacct
     4741 ttagtgacag tagtgcctca aaggaagatg aaaactatcg tatcttctcg ttactggaaa
     4801 acgccgaaga agaaaaagaa cggcaaatag cctcaatact ttcatgggaa atagatatta
     4861 tctataaaat cctgctggat tctgatttag gcagtagttt gcctttaagc caggctgatt
     4921 ttggcctgtg gtttaaccat aaaggtcgac attattttag cggcattgcc gaagtaggcc
     4981 atatctcccg tctgattcag gatttcgacg gtattttcaa tcaaaccatg cgtaacacca
     5041 ggaatttgaa taacagaagt ttgcgggtga agtttctatt acagataaga aataccgtat
     5101 cgcaaattat taccttgctg cgtgaattgt ttgaagaagt atcgcgccac gaagtcggta
     5161 tggatgtact gacgaaatta cttaaccgcc gtttcctgcc gactatcttc aaacgcgaaa
     5221 ttgcccatgc caaccggacc ggtacaccgc tgtcagtgct gattattgac gttgataaat
     5281 tcaaagagat caacgatacg tggggccata acactggcga tgaaattctg cgtaaagtct
     5341 ctcaggcctt ttatgacaac gtccgcagta gtgattatgt tttccgctac gggggcgatg
     5401 aatttatcat tgttttgact gaagcttctg aaaacgaaac gttacgtacc gcagaacgta
     5461 ttcgcagtcg ggtggagaaa accaaactga aagccgcaaa cggcgaggat attgccctct
     5521 cactttccat cggtgccgcc atgtttaatg gtcatcctga ctatgagcgc ctcattcaaa
     5581 tagccgatga agctctgtat atcgccaaaa gacgaggtag aaaccgtgtt gaactctgga
     5641 aagccagtct ttagatgcgc caggatgcag aggtaatcat gaagctaacc gatgcggata
     5701 atgccgccga tggcattttt ttccccgccc ttgagcaaaa tatgatgggt gcggtgttaa
     5761 ttaacgaaaa tgatgaagtg atgtttttca accccgccgc agagaagctc tgggggtaca
     5821 aacgtgaaga agtcattggc aataacattg atatgctgat tccgcgggat ttgcgtcctg
     5881 cgcatcctga atacattcgt cataaccgtg aaggcggtaa agcgcgtgtc gaggggatga
     5941 gtcgggagct gctgctggag aaaaaagacg gcagtaaaat ctggacccgt tttgcgctat
     6001 cgaaagtgag cgccgagggg aaagtttatt acctggcgct ggtacgggat gccagcgtag
     6061 aaatggcgca aaaagaacag actcgacaat tgattattgc cgttgaccat ctcgaccgac
     6121 cggtgattgt cctcgatccg gaacgccata ttgtgcagtg caatcgcgca tttaccgaaa
     6181 tgtttggtta ctgcattagc gaagccagcg gtatgcagcc cgatacactc ctgaacattc
     6241 ctgaattccc tgccgataac cgcattcgtt tacaacagtt gctatggaaa accgcccgcg
     6301 atcaggacga atttctgctg ttgacgcgca ccggtgaaaa aatctggatt aaagcctcta
     6361 tcagcccggt ttatgacgtg ctcgcgcatc tgcagaacct ggtaatgact ttctcggata
     6421 tcaccgaaga acggcaaatc cgccagcttg aaggcaatat tctcgccgcc atgtgcagca
     6481 gcccgccatt tcatgaaatg ggggaaatca tttgtcgtaa catcgaatct gttctcaacg
     6541 aatcgcatgt ttcgctgttc gcactgcgca acgggatgcc gatacactgg gcgtcatctt
     6601 cgcacggtgc agaagttcaa aatgcgcaaa gctggtcagc gaccattcgt cagcgtgatg
     6661 gcgcgcctgc ggggatcctg caaattaaaa cctcgtcagg agcagaaacc agcgccttta
     6721 tcgaacgcgt ggcagatatc agtcagcata tggccgcgct ggcgctggaa caggaaaaaa
     6781 gccgccagca tattgaacaa ctcatccaat ttgatccgat gaccggtctg ccaaatcgca
     6841 ataacctgca caattacctc gatgacctgg tcgacaaagc cgtctctccg gtggtgtatc
     6901 tcatcggtgt tgaccatatt caggatgtga ttgatagcct tggctatgcg tgggccgatc
     6961 aggcattgct ggaagtggtc aatcgctttc gtgaaaaact caaaccggat cagtatctct
     7021 gtcgtatcga aggtacgcag tttgtcctcg tgagcctgga aaacgacgtc agtaacatta
     7081 cccaaatcgc cgatgagcta cggaatgtgg tcagcaagcc gataatgatt gacaataaac
     7141 ccttcccgct taccttgagt attggcatca gctacgacgt gggtaaaaac cgcgattacc
     7201 tgctctccac tgctcacaat gcaatggatt atattcgcaa gaatggcggt aacggctggc
     7261 agttcttcag cccggcgatg aacgaaatgg taaaagagcg tttggtttta ggcgcagcgc
     7321 tgaaagaagc gattagcaat aaccaactga aacttgttta ccagccgcaa atcttcgcag
     7381 aaacgggtga actgtacggc atcgaagccc ttgcgcgctg gcacgatccc cagcatggtc
     7441 atcatatccc ggcgctttcc gttaacttgt cggcactgca ctttcgcagt aatcaactgc
     7501 ctaatcaggt gtctgatgca atgcacgcct ggggtattga cggccaccag ctgacggtag
     7561 aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc attcagatcc
     7621 tgcgtgatat gggcgtgggc ttatcggtag atgattttgg tacgggcttt tccggattat
     7681 cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagtttt gtcgatcgtt
     7741 gtctgaccga aaaacgcatc cttgccttac ttgaagccat taccagcatt gggcaaagcc
     7801 tcaatttaac cgtcgtggcg gaaggcgtcg aaaccaaaga gcaatttgag atgctacgca
     7861 agatccactg tcgcgttatt cagggatatt tcttttcccg ccccctaccc gccgaagaaa
     7921 ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct ctcgcccgca
     7981 ctcgcgggtt ttcatttaac gtgacactgt cacttaaata ctgtgatttc agctacgact
     8041 ccgggagatt ccttccttaa cttcccctct tgcaccaaaa ctgcactacg ctgcacaaat
     8101 gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta tcccggcatg
     8161 gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg aaccgtttat
     8221 gtcggatacc accgaactgg ttgatttagc cgtaatcttc cctgatctgg agagcgaatt
     8281 gaaatacgcc tgcgctgata acatcacagg taaagctatt tatcagcaag cgcgttgtct
     8341 gctacacaag gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc agctgtcagg
     8401 cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga tgttgtggca
     8461 agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc acagccgtgg
     8521 cacggcgatc gacctgacgc ttcgtgatga gcacgggaac atcctcgata tgggcgcagg
     8581 gttcgatgaa atgcacgagc gctcccatgc ctatcaccct tccgtcccgc ccgccgctca
     8641 gcgcaatcgg ctgttgctga atgcgataat gactggtggc ggttttgtcg gtatctccag
     8701 cgaatggtgg cacttcgaat taccccaggc agcgagttac cctctgctcg ctgatcaatt
     8761 cacctgtttt atatcccctg gcacacaaca cgtcagttaa tcaggaaact gtcatgaaga
     8821 gatcgatacc gtttcgtccc acattgctcg cgctcgtcct tgccacaaat ttcccggttg
     8881 cgcacgccgc cgtaccgaaa gatatgctgg tgattggtaa ggccgccgat ccacaaaccc
     8941 tcgacccggc ggtaacaata gataataacg actggacggt gacctacccg tattatcagc
     9001 ggctggttca gtacaaaacg gacggtgata aaggctcacc cacgttgaag gcgatctggc
     9061 aagtagctgg aaagcgtctg acgatcaaaa agagtggacg ttcaccctga aggacaatgc
     9121 taaatttgcc gatggcacac cggtcactgc cgaagcagta aaactctctt ttgagcggct
     9181 actaaaaatc ggccaggggc cagcagaagc atttcccaaa gatttaaaga ttgatgctcc
     9241 cgacgaacat acggtgaagt ttacccttag ccagccattc gcaccgttcc tctacacgct
     9301 ggcgaatgac ggtgcatcca ttatcaatcc ggcggtctta aaggaacatg cagcggatga
     9361 tgctcgcggc ttcctcgcgc aaaataccgc cggttccgga ccatttatgc tgaaaagctg
     9421 gcaaaaaggt cagcaattag ttctggtgcc aaatccgcat tacccaggca ataaaccgaa
     9481 cttcaaacgg gtatcggtaa aaattattgg tgaaagtgcc tcccgtcgcc tgcagctctc
     9541 ccgtggcgac attgacattg ccgatgcgct gccggtggat caactcaacg ccctgaagca
     9601 ggaaaataaa gtcaatgtgg cagagtatcc gtcactgcgc gttacctatc tgtatctgaa
     9661 taacagcaaa gcgcctctta atcaggcgga tctgcgtcgg gccatttcct ggtctaccga
     9721 ttatcaggga atggttaacg gcattctgag tggtaacgga aaacagatgc gcggcccgat
     9781 tccggaaggc atgtggggct acgatgcgac ggcaatgcaa tacaaccatg acgaaacgaa
     9841 agccaaagcc gaatgggata aagtgacgag caaacccacc agcctgacgt ttctctactc
     9901 cgataacgat ccgaactggg agcctattgc tctggcgaca caatccagtc tcaacaagct
     9961 gggcatcaat gtgaagctgg aaaagctggc gaacgccacc atgcgcgaca gagtgggtaa
    10021 aggtgattac gacattgcga ttggcaactg gagtccggat tttgccgacc cgtatatgtt
    10081 tatgaattac tggtttgagt cagacaaaaa aggtctgcca ggcaaccgct cgttctatga
    10141 aaacagtgag gtcgataagt tactgcgcaa tgcgcttgcg accaccgacc agacgcagcg
    10201 tacccgggac taccagcagg cacagaaaat cgtcattgat gacgctgctt atgtgtacct
    10261 attccagaaa aactaccaac tagcgatgaa caaagaggtg aaaggctttg tgttcaatcc
    10321 catgctggaa caggtcttca atatcaatac catgagtaaa taacgtgacc ttctggagta
    10381 ttttacgcca acgctgctgg gggctggtgc tcgtggtggc gggcgtctgc gtgattacgt
    10441 ttattatctc gcatctgatc cctggcaatc cggcgcggtt actggcgggt gaccgcgcca
    10501 gcgatgctat cgtggaaaat attcgccagc aactgggact ggaccagcca ctgtatgtac
    10561 agttttaccg ctacgtcagc gacctgtttc atggtgacct gggaacatcc attcgtaccg
    10621 ggcgtccggt gctggaagag ttgcgtatat ttttcccggc gacgctggaa ctggcttttt
    10681 gtgccctgct gctggcactc ctgattggca tcccgctggg catactctct gcagtctggc
    10741 gaaatcgctg gctggatcat ctggtgcgaa taatggccat taccggaatc tccacacctg
    10801 cgttctggct tggactgggc gtcattgtgc tgttttatgg tcatctgcaa atacttcccg
    10861 gcggcggaag gcttgatgac tggctggatc caccaacgca cgttaccggc ttttatctgc
    10921 tcgatgcgct gcttgaaggc aacggtgaag tcttcttcaa tgcgttgcaa catctcatct
    10981 taccggcatt aacgctggcg ttcgttcacc tgggaattgt cgcccgccag attcgctcag
    11041 cgatgctgga acaattgagt gaagactaca ttcgtaccgc ccgggccagc ggcttgcccg
    11101 gctggtatat cgttttatgt tatgcgctac ccaatgcgtt gatcccatcg attaccgtat
    11161 tgggtttggc gctgggcgat ttgttgtatg gcgcagtgct caccgaaacc gtttttgcct
    11221 ggcccggaat gggtgcatgg gtagtaacat caatacaggc gctcgacttc ccggcagtga
    11281 tgggctttgc cgtcgtggtt tcatttgctt atgtgctggt caacctggtg gtggatttgc
    11341 tctatttgtg gattgatccg cgtatcggac gtggaggtgg tgaatgatgc taagcgagga
    11401 aacgtccgcc gtacgcccac aaaaacaaac gcgatttaac ggtgcaaaac tggtatggat
    11461 gctgaaaggc agtccgctca ccgtgaccgg cgcagtcatc attgtattaa tgctattgat
    11521 gatgattttt tcaccgtggc tggcgacgca tgatcccaat gccattgatt taaccgcccg
    11581 ccttttgccg ccttctgcgg cgcactggtt tggcaccgat gaagtgggac gcgatctgtt
    11641 cagccgcgta ctggtcggca gtcagcaatc aattctcgcc ggattagtcg tggttgccat
    11701 tgcgggtatg attggttcgc tactcggatg tctatccggt gtgcttggcg gacgcgcaga
    11761 cgccatcatc atgcgcatca tggacattat gctgtcgatt ccttcgttgg tactgacaat
    11821 ggcactggct gccgctctcg ggccgagttt atttaacgcc atgctggcga ttgctattgt
    11881 gcgaattccc ttttatgtgc gcctggcgcg ggggcaagca ttagttgtac gccagtatac
    11941 ctatgttcag gcggcgaaaa cctttggtgc gtcgcgttgg catctgatca gctggcatat
    12001 tttacgtaac tccctgccgc cgctgatcgt gcaggcatcg ctggatatcg gtagcgcgat
    12061 tttaatggcc gccacgttgg gatttattgg cctgggtgct caacaaccga gtgctgaatg
    12121 gggcgcgatg gtagcgaatg gtcgcaacta tgtactcgat cagtggtggt attgcgcatt
    12181 tccgggggca gcgattttgc ttaccgccgt tgggtttaat ctctttggtg atggtattcg
    12241 cgatctgctt gacccgaaag caggaggaaa gcagtcatga cccaacccgt tctggacatt
    12301 caacagctgc atttgagttt ccccggtttt aacggtgatg ttcacgcgct caacaatgtg
    12361 tccttgcaga ttaaccgcgg tgaaattgtc ggtctggtgg gagaatccgg ctcaggtaaa
    12421 tcagtcaccg caatgctgat tatgcgtctg ctaccgacgg gcagttattg cgtacatcgg
    12481 gggcagattt cactgctggg agacgatgtt cttaacgctc gggaaaaaca gcttcgtcag
    12541 tggcgcggcg cacgagtggc gatgatcttt caggaaccga tgaccgccct caatccgaca
    12601 cgtcgaatag gtcttcagat gatggacgtg atccgccatc atcaaccaat aagtcgtcgg
    12661 gaagccagag ctaaagcgat tgccctgctg gaagagatgc aaatcccgga tgccgtggaa
    12721 gttatgtcgc gctatccgtt tgagctttca ggtggtatgc gccagcgggt aatgattgcg
    12781 ctggcattct cctgcgagcc gcaattgatt attgccgacg aaccgactac ggcgctggac
    12841 gtcacgctac agttgcaggt actgcgtttg cttaaacata aagcccgcgc cagtggaact
    12901 gcggtactgt tcatcagcca tgatatggtc gtggtgtcgc aactgtgcga tagcgtttac
    12961 gtgatgtatg ccggaagcgt aattgaaagc ggcgtgacgg cagacattat ccatcatccc
    13021 cggcatccgt ataccattgg tttgctgcaa tgcgcaccag aacatggagt accacgccag
    13081 ccattacccg ccattccagg gacggtacca aacctcaccc atttgcctga aggctgcgct
    13141 tttcgcgatc gttgctatgc ggcaggtgca cagtgtgaaa acgtcccggc gctgacagcg
    13201 tgtggtgaca acaaccagcg ctgcgcctgt tggtatccgc agcaggaggt cataagtgtc
    13261 taacacgtta ttaacgttat gcgacgtcca tatcaatttc ccggcccgta aaaactggct
    13321 tggtaaaact acggaacatg ttcatgccat taatggtatt gatttacaga tccgccgtgg
    13381 tgaaacctta gggatcgtcg gcgagtcagg ctgcggcaaa agcaccctcg cacagctttt
    13441 aatgggtatg ctgcaaccga gccacgggca gtacatccgt tcaggatcac aacgcattat
    13501 gcagatggtg tttcaggacc cgctctcttc gctcaatccg cgcttaccgg tgtggcgcat
    13561 catcacagaa ccgctctgga tagctaagcg tagtagtgaa caacagcggc gagcgttagc
    13621 agaggagttg gctgtgcagg tgggtattcg tccggagtat ctcgaccgcc tgcctcatgc
    13681 gttctccggc gggcagcggc aacgcatcgc cattgccaga gcactctctt cgcagcctga
    13741 cgtgattgtg cttgatgagc aaacctctgc actggatatc tccgtgcagg cgcagatcct
    13801 caatttactg gtaacgctac aggtaaatcg cgggctgacc tatgtgctga tctcacacaa
    13861 tgtctcggtg attcgtcata tgagcgatcg ggtagcggtg atgtatctcg ggcagattgt
    13921 agaactgggc gacgcgcagc aggtgttgac ggcacctgca catccataca cccgattatt
    13981 gctggattcc ctccccgcca ttgataaacc gctggaggaa gaatgggcat tacgtaaaac
    14041 ggatctgccg ggaaaccgca cgttgccgca aggctgtttt ttctacgaac gttgccctct
    14101 ggcaacccac ggatgtgaag tccggcaatc attaacaata agggaggacg gacgtgagat
    14161 ccggtgctgg cgggcgctgt agagatgcta acaacgcatc aggcattcac gcgcctgacg
    14221 cgtcatccgg caatgcttta cgatttcaac tggtaatcaa gcgtaatttc cgctttcagt
    14281 acctgagaaa ccgggcatcc tgcttttgct ttctggatga tgccgtcaaa ggtagaggca
    14341 tcaatacccg gcaccgcaac ttcactcttc agtgcgattt tcgtaatcgc aaaaccggca
    14401 tccactttat ccagcgacac atcggcggtg gtatcaatcg atgttggcgt aaatcccgct
    14461 tcccccagca ttaatgaaag cgccattgag aaacatgcgg catgcgctgc gccaatcagt
    14521 tcttcagggt tagttccttt ttcgccttca aaacgcgtgt taaatccata cggctgttgg
    14581 ttcagcacac cactctcggt ggatactgtt cccttcccgc gtttgatatc gccttcccag
    14641 tgtgcctgac ctttcttatg gattgtcatt gttgctctcc tgtgggctta aaaaggaaat
    14701 tatagactac gtgagaaaca gcacgaggat aagcaggata ttccgaataa aaatccggca
    14761 gaaatattac ttctgcactt aaattaaatt tccacaatag ataaccggga atatataacc
    14821 caagagatat tgcattaata ttaatcaact caccacaata ataataccta tcaatatcat
    14881 gaggttaaaa tataaaaatc atcaaccagg actaatctta acaacgaagc ggcaaatatt
    14941 tgccatgctg aatgtgcttt ataaatctgc gatccgtagc agacaccata aatacacaga
    15001 cacggagaat cactatgttt acttattatc aggcagaaaa ttcaacagca gaacccgctc
    15061 tggttaatgc cattgagcaa ggccttcggg cagagcacgg tgttgtcact gaagatgaca
    15121 ttctcatgga gctgaccaaa tgggtggaag cttctgataa cgacatcctc agtgatatct
    15181 accagcaaac gatcaattat gtggtcagtg gccagcaccc tacgctttaa ggtgctatgc
    15241 ttgatcggca acctaattta ggggtttagc acgtgtttct tcgctacggc gatgttgtcc
    15301 ttaaaactag caacaggatt gaggagttaa aatgaaatcg aaccgtcagg cacgtcatat
    15361 tcttggactg gaccataaaa tttctaacca gcgcaaaata gttaccgaag gtgacaaatc
    15421 cagcgtggta aataacccaa ccggcagaaa acgccccgct gaaaagtaat tcataaccat
    15481 cagtcctcaa tgacgattaa acaccattgc ctgcgcaatg gtgtttttgt ttttatctgc
    15541 tttatacttg gggccgacgc cctggcggta aagcaaagac gataaaagcc ccccagggat
    15601 ggatattcaa aaaagagtga gtgacatgga accaaaaaca aaaaaacagc gttcgcttta
    15661 tatcccttac gctggcccgg tactgctgga atttccgttg ttgaataaag gcagtgcctt
    15721 cagcatggaa gaacgccgta acttcaacct gctggggtta ctgccggaag tggtcgaaac
    15781 catcgaagaa caagcggaac gagcatggat ccagtatcag ggattcaaaa ccgaaatcga
    15841 caaacacatc tacctgcgta acatccagga caccaacgaa accctcttct accgtctggt
    15901 aaacaatcat cttgatgaga tgatgcctgt tatttatacc ccaaccgtcg gcgcagcctg
    15961 tgagcgtttt tctgagatct accgccgttc acgcggcgtg tttatctctt accagaaccg
    16021 ccacaatatg gacgatattc tgcaaaacgt accaaaccat aatatcaaag tgattgtggt
    16081 gactgacggt gaacgtattc tggggcttgg tgaccagggc atcggcggga tgggcattcc
    16141 gatcggtaaa ctgtcgctct ataccgcctg tggcggcatc agcccggcgt atacccttcc
    16201 ggtggtgctg gatgtcggaa cgaacaacca acagttgctt aacgatccgc tgtatatggg
    16261 ctggcgtaat ccgcgtatca ctgacgacga atactatgaa ttcgttgatg aatttatcca
    16321 ggctgtgaaa caacgctggc cggacgtgct gttgcagttt gaagactttg ctcaaaaaaa
    16381 tgcgatgccg ttacttaacc gctatcgcaa tgaaatttgt tcctttaacg atgacattca
    16441 gggcactgcg gcggtaacag tcggcaccct gatcgcagca agccgcgcgg caggtggtca
    16501 gttaagcgag aaaaaaatcg tcttccttgg cgcaggttca gcgggatgcg gcattgccga
    16561 aatgatcatc gcccagaccc agcgcgaagg attaagcgag gaagcggcgc ggcagaaagt
    16621 ctttatggtc gatcgctttg gcttgctgac cgacaagatg ccgaacctgc tgcctttcca
    16681 gaccaaactg gtgcagaagc gcgaaaacct cagtgactgg gataccgaca gcgatgtgct
    16741 gtcactgctg gatgtggtgc gcaatgtaaa accagatatt ctgattggcg tctcaggaca
    16801 gaccgggctg tttacggaag agatcatccg tgagatgcat aaacactgtc cgcgtccgat
    16861 cgtgatgccg ctgtccaacc cgacgtcacg cgtggaagcc acaccgcagg acattatcgc
    16921 ctggactgaa ggtaacgcgc tggtcgccac tggcagtccg tttaatccag tggtatggaa
    16981 agataaaatc taccctatcg cccagtgtaa caacgccttt attttcccag gcatcgggct
    17041 gggtgttatt gcttccggcg cgtcacgtat caccgatgag atgctgatgt cggcaagtga
    17101 aacgcttgct cagtattcgc cgctggtcct gaacggcgaa ggtctggtac taccggaact
    17161 gaaagatatt cagaaagtct cccgcgcaat tgcgtttgcg gttggcaaaa tggcgcagca
    17221 gcaaggcgtg gcggtgaaaa cctctgccga agccctgcaa caggccattg acgataattt
    17281 ctggcaagcc gaataccgcg actaccgccg tacctccatc taagcctgcg cccggtagtg
    17341 aaggctaccg ggctatttcc ctctcccttt ttcagatctc atccatactg ggtagtggcg
    17401 aataaatctc atttgcctca cctgctatgc agaacatcat ccgaaaagga ggaactatga
    17461 aggctgcagt tgttacgaag gatcatcatg ttgacgttac ggataaaaca ctgcgctcac
    17521 tgaaacatgg cgaagccctg ctgaaaatgg agtgttgtgg tgtatgtcat accgatcttc
    17581 atgttaagaa tggcgatttt ggtgacaaaa ccggcgtaat tctgggccat gaaggcatcg
    17641 gtgtggtggc agaagttggc ccaggtgtca cctcattaaa accaggcgat cgtgccagcg
    17701 tggcgtggtt ttacgaagga tgcggtcatt gcgaatactg taacagtggt aacgaaacgc
    17761 tctgccgttc agttaaaaat gccggataca gcgttgatgg cgggatggcg gaagagtgca
    17821 tcgtggtcgc cgattacgcg gtaaaagtgc cagatggtct ggactcggcg gcggccagca
    17881 gcattacctg tgcgggagtc accacctaca aagccgttaa actgtcaaaa attcgtcccg
    17941 gtcagtggat tgctatctac ggtcttggcg gtctgggtaa cctcgccctg caatacgcga
    18001 agaatgtctt taacgcgaaa gtgatcgcca ttgatgtcaa tgatgagcag ttaaaactgg
    18061 caaccgaaat gggtgcagat ttagcgatta actcacgcac cgaagacgcc gccaaaattg
    18121 tgcaggagaa aaccggtggc gctcacgctg cggtggtaac agcagtagct aaagctgcgt
    18181 ttaactcggc agttgatgct gtccgtgcag gcggtcgtgt tgtggctgtc ggtctgccgc
    18241 cggagtctat gagcctggat atcccacgtc ttgtgctgga tggtattgaa gtggtcggtt
    18301 cgctggtcgg cacgcgccag gatttaactg aagccttcca gtttgccgcc gaaggcaaag
    18361 tggtgccgaa agtcgccctg cgtccgttag cggacatcaa caccatcttt accgagatgg
    18421 aagaaggcaa aatccgtggc cgtatggtga ttgatttccg tcactaagag gcctttgctg
    18481 cgactgccat gttcgggtcg cagcatcgca cactctccaa catgaaatgg ctaaatgaat
    18541 tattgttgcg taagaaggaa caataatgat actgttccca gtgaaggaac actactgaga
    18601 gtaaagacac tgatgaaaat ggccaatcat ccccgcccgg gggacattat tcaggaatca
    18661 ctggacgaac ttaatgtcag cctgcgcgag tttgccagag caatggaaat tgctccctca
    18721 acggcaagcc gattgctgac cggaaaagca gctttgacgc cagaaatggc aattaaactt
    18781 tccgtggtga tcggcagttc gccgcaaatg tggctgaatc tgcaaaacgc ctggagtctg
    18841 gcagaggctg aaaaaacggt ggatgtgtcg cgattacgcc gtctggttac gcaatagatt
    18901 acgccgtctg gttacgcaat aaaaaatggc accttataaa gcgccatttg aaactgatga
    18961 caaacgcaac attgcctgat gcgctacgct tatcaggcct acacgttcac tgcaatctat
    19021 tgtatttgaa tggttttcat gccggataag gcattcacga cgcatccggg atgaacaaag
    19081 cccacgttgt ctacaatatg aaaatgacgc cttttacagc gccattttca gcttaaagtt
    19141 caaaagagat tatatccctt cttcactctc ttttttcgct tctgccttct cgatttcacg
    19201 ataccagcgc ggatggtgtt tcttcgccca gcgacggctc accttccctt cgatcatccc
    19261 tttaatcgat cctttcaccc agaatgccat atacatatgg atcaggatgg cgtggatcag
    19321 gatgataccc gcagccgcgt ggatcagcag gctataacgg acaacctgca tcgggaagta
    19381 ctgcgcaaag tacggacgcc agataatcac cccggtcacc agcagcacga aaatcatgct
    19441 catgattgac cagaacatca tcttttgccc ggcgttgtac ttaccgacat ccgccacttt
    19501 atgctcattg cctttcagta cttcgacaat gttcaacagc cacggaatat ctttcttatc
    19561 cgggatgttg tgatgcacaa aacgcacaaa cataaacatc agcgcgacga aaatcgcaat
    19621 gccgaagaac gggtgcaaaa tacgtcccat ctgcggcgta ccgaaggttt gcgtcagcca
    19681 ttgcagcgtc gggaagaaga acgaaatccc ggacagcgcc accaggaaga agcaaatcac
    19741 cacggtccag tgacaggcgc gatcaataaa tttggtgcgc acaatcattt tcgacttact
    19801 catgatgatc ctcctcgtca tcgtccactt ccttattcgg gccaataccg atgtagtgga
    19861 aaatcaaccc ggcaaaagtg gcaataaagc cagccgctgc cagcggtttc aacgcgcctt
    19921 tccacaggct taccgaggtg tcgatcttcg gatctttcgg cagaccgtga tacagctccg
    19981 gctgatcggc gtgatgcagc acgtacataa cgtgcgtacc accgacccct tccgggttgt
    20041 agacgccagc atgttcgtaa ccacgcgctt tcagtttcgc cacgcgctgt tccgccagct
    20101 ccagcatctc cttcttggtg ccgaagtgga tagccccggt cggacaggtt ttcacacaag
    20161 ccggttcctg gccgacgctg acgcgatcga cgcagagcgt gcatttatat acccggttat
    20221 cctctttgtt gaggcgcgga atattaaacg gacacccggc aatgcagtaa ccacagccga
    20281 tgcagttttc cgactggaaa tcgacaatcc cgttagcgta ctgaatgatt gcaccagcag
    20341 acgggcacgc cttaaggcag cccggatctt cacagtgcat acagccgtct ttacggatca
    20401 gccactccag cttgccgttc tgttcggttt cgctaaagcg catcaccgtc caggacttgg
    20461 cgctcagatc ggcggggtta tcgtaaaccc cgacgcagtg ccccacttca tcacggatgt
    20521 cgttccactc cgaacacgcc acctgacagg ctttacagcc gatacaggtg gaaacgtcga
    20581 taagtttcgc gacttctgct ttgtaatcac gcacctgaga aggcggcgtg atggagttag
    20641 ttgcggacct tttgataatg tcctgcgttt ccatagccat ctgttcgccc ccttacgcct
    20701 tctcgatgtt gactaagaac gctttatatt ccggcgtttg cgagtttgca tcaccgacat
    20761 tcggcgtcag agtgttagcg atataacctt tacgcgcgac accctcaaag ccccagtgga
    20821 ttggaatacc caccgtttca acctgctgac cgtttacatt cagcggtttc agacggcgcg
    20881 ttaccaccgc caccgcgcgg ataaagccac gcttgcttga gacagtgaca cgatcgccat
    20941 tattaatgcc tttcgccgcc gccagcgttt cgctgatttc cacaaactgt tccggctgag
    21001 caattgcgtt gagcaatgcg tgcttggtcc aggtgtggaa gtgctcggtc agacgatagg
    21061 tcgtacccac atacgggaac tgctcttttt tacccatccg cagtgcgtct tgttcataca
    21121 gacgaacaac cgggttagac accacgttcg gatgcagcgg gttagtgccc agcggcgttt
    21181 caatcggctc gtagtgttcc gggaacggac cttccgccat tttgttgatg gcaaacaggc
    21241 gtcccatccc ttccggctgc atgataaacg gcccggttgg cgtacccggt gcggcattgc
    21301 cgaagtcagg aatatcgtta cccgtccact tgctgccgtt ccactggatc agcatccgtt
    21361 tcggatccca cggtttaccg ttgatatccg ccgaagcacg gttgtacagc acgcgacggt
    21421 tgagcggcca cgcccaggcc catcccagcg tattccccag accggacggg tcggagttat
    21481 cgcggttagc catctggttg ccctgctctg tccagctacc ggtgtagatc cagcaagaag
    21541 atgcggttgt accgtcatca cgcagatgcg caaagctact cagcaactga cctttcttcg
    21601 caatcagcac gccattagca tcatagagat cttccagcgc atagccgttg ttctctttag
    21661 ctacttcgtc agattgcggt tcgtgcggct gcttgtagtt ccagctcatc ttcatcagcg
    21721 gttctacgcc tttaccacct tcggcctggt acagctcgcg caggtgatgg tagatacccg
    21781 ccagaatttc gccgtcgtta cgcgcttcgc ccggcgcgtc ctgacctttc cagtgccact
    21841 gcaaccagcg accggagtta gcgatagaac catcttcttc ggcaaagcag gtcgaaggca
    21901 gacggaatac ttcagtctga atagacgccg gatcgacatc gttcgactcg ccgtggttct
    21961 gccagaaggt agaagtttca gtcaccagcg gatcgataac caccatgtac ttcagcttgc
    22021 tcaggcagct caccactttg tttttgtccg ggaaggacgc aaccgggtta aagccctggc
    22081 agaaataacc ggtgactttg ccttcatcca tcatgttgaa atacttgatg acgtcgtagg
    22141 tctggtccca cttcggcagc cagtcatagc cccagttgtt ctctttctgc gcggcatcgc
    22201 catagaaaga tttcatcagg ctaacgaaga acttcggata gttgctccag tagttcacct
    22261 gatcagccag cgtcgctttc ggcgtgttcg cttccagata cgactgcaaa tcaacctgtt
    22321 tttctgacgg cagcgtcaga taacctggca ggctggtaga gagcaggcct aagtcagtca
    22381 gaccctgaat gttggagtga ccacgcaatg cgttcacgcc gccaccggcc atacccatgt
    22441 taccgagcag caactggatc atcgccatag tacggatgtt ctgcgcaccc acggtgtgct
    22501 gcgtccagcc cagcgcgtac aggaaggtgg ttgtgcgatc cggtgcgctg gtggaggcca
    22561 gcacttcaca cactttcagg aagtcggctt ttggcgtacc acagatgttt tcaacaacgt
    22621 ccggcgtgta gcgggaaacg tgcgctttca gcaggttcca cacacagcgc ggatgagtca
    22681 gtgtttcatc gcgtttcgca tagccgtttt catcgaactg atagttccag gacgatttat
    22741 cgtattgccg tttttcagcg tcgtagccgc tgaacagacc gtcttcgaaa gcaaaatcat
    22801 cacgcaccag caggctggcg ttggtgtaat gtttaacgta ttcggcgttg attttgttgt
    22861 tttcgatcag gtagcgcaaa acgccagaca ggaacgtaat gtccgtaccg gaacgaatag
    22921 gtgcgtaaat atccgccaca gaagcggtac gcgtaaaacg gggatcgaca acgatcaagg
    22981 ttgcatcgtt gttgtttttc gcttccatcg cccagcggaa accgacggga tgcgcttcag
    23041 cagcgttacc gcccattacc attacgacgt tagcgttttt gatatccacc cagtggttgg
    23101 tcatcgcacc gcgaccaaat gttggagcaa gacttgctac cgttggtccg tgtcagacgc
    23161 gcgcctggtt gtctaccgcc agcatcccga gggagcgggc aaatttctgt gtcagcatcc
    23221 cggtttcgtt gctggcaccg gaggcacaca gcataccggt agaaagccaa cggtttaccg
    23281 ttacgccctg ctcgttcttt tcaataaagt tagcgtcacg gtcagctttc atcagctttg
    23341 caatacggga gaatgcttct tcccagctaa tgcgctgcca tttgtcagaa cctggcgcac
    23401 gatattccgg gtagcgcaga cggttttcgc tgttgacgta atccagcaaa ccggcccctt
    23461 ttgggcacag cgcaccacgg cttaccggat gatccgggtc accttcaatg tgataaatcg
    23521 cttctctggc gtttttcgcg ccatcaccca ggctatacat caatagcccg caacctacgg
    23581 aacagtatgt gcaggtgtta cggatctctt tagcgcgtaa taatttgtag tttcgcgcct
    23641 gagccagtgc ttgcttcggg gcaaagccca atgccgctac cgttgttcca gccataccgc
    23701 ccgcgcagat tttaaaaaat tgtctgcgac tgacgtccat tgctttcctc ttttttcagg
    23761 ggtattactg cgcgaggaaa ctaacagtaa aattctgaat ctttttgcgc gaaatcaaaa
    23821 agaacacgtg tttgccacaa aataatccgc ctgcgcaaat tgacctacct caatagcggt
    23881 agaaaaacgc accactgcct gacaggccag ttaaaaaaat gctataaaat tcagcttaat
    23941 ttttaacggc aagagagaca aaacagcgag catgacacga caaaaagcaa cgctcatagg
    24001 gctaatagcg atcgtcctgt ggagcacgat ggtaggattg attcgcggtg tcagcgaggg
    24061 gctcggcccg gttggcggcg cagctgctat ctattcatta agcgggctgc tgttaatctt
    24121 cacggttgga tttccgcgta ttagacaaat cccgaaaggc tatttactcg ccgggagtct
    24181 gttattcgtc agctatgaaa tctgtctggc gctttcctta gggtatgcgg cgacccgtca
    24241 tcaggcgatt gaagtgggta tggtgaacta tctatggccc agcctgacaa ttctctttgc
    24301 cattctgttt aatggtcaga aaaccaactg gctgattgta cctggattat tattagccct
    24361 tgtcggcgtc tgttgggtgt taggcggtga caatgggtta cattatgatg aaatcatcaa
    24421 taatatcacc accagcccat tgagttattt cctggcgttt attggtgcgt ttatctgggc
    24481 agcctattgc acagtaacga ataaatacgc acgcggattt aacggaatta ccgtttttgt
    24541 cctgctaacg ggagcaagtc tgtgggttta ctattttctt acgccacaac cagaaatggt
    24601 atttagcacg cccgtcatga ttaaactcat ctctgcggca tttaccttag gatttgctta
    24661 tgctgtatgg aatgtcggta tattgcatgg caatgtcacc ataatggcgg taggctcgta
    24721 ttttacgcct gtactttcct cagcgcttgc agccgtgctg ctcagcgccc cgctgtcgtt
    24781 ctcgttctgg caaggcgcgc taatggtctg cggcggttcc ctgctctgct ggctggcgac
    24841 acgtcgtggt taaaataatt gccgggtcta cggcccggca gttaatcaga taaattccga
    24901 cagtattaaa aaataaattt caaataccag taattcacct tatatctc
//
LOCUS       DAZGSP010000073        24571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZGSP010000073 DAZGSP010000000
VERSION     DAZGSP010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.073"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(29..105)
                     /locus_tag="ACHK74_003297"
     tRNA            complement(29..105)
                     /locus_tag="ACHK74_003297"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69..71),aa:Arg,seq:acg)
     gene            complement(303..379)
                     /locus_tag="ACHK74_003298"
     tRNA            complement(303..379)
                     /locus_tag="ACHK74_003298"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(343..345),aa:Arg,seq:acg)
     gene            complement(383..475)
                     /locus_tag="ACHK74_003299"
     tRNA            complement(383..475)
                     /locus_tag="ACHK74_003299"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(439..441),aa:Ser,seq:gct)
     gene            complement(791..976)
                     /gene="csrA"
                     /locus_tag="ACHK74_003300"
     CDS             complement(791..976)
                     /gene="csrA"
                     /locus_tag="ACHK74_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFP0222062.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1211..3841)
                     /gene="alaS"
                     /locus_tag="ACHK74_003301"
     CDS             complement(1211..3841)
                     /gene="alaS"
                     /locus_tag="ACHK74_003301"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFP0222063.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3969..4469)
                     /gene="recX"
                     /locus_tag="ACHK74_003302"
     CDS             complement(3969..4469)
                     /gene="recX"
                     /locus_tag="ACHK74_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFP0222064.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4712..5773)
                     /gene="recA"
                     /locus_tag="ACHK74_003303"
     CDS             complement(4712..5773)
                     /gene="recA"
                     /locus_tag="ACHK74_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP0222065.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5853..6350)
                     /gene="pncC"
                     /locus_tag="ACHK74_003304"
     CDS             complement(5853..6350)
                     /gene="pncC"
                     /locus_tag="ACHK74_003304"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFP0222066.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6495..7580)
                     /gene="mltB"
                     /locus_tag="ACHK74_003305"
     CDS             complement(6495..7580)
                     /gene="mltB"
                     /locus_tag="ACHK74_003305"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFP0222067.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7836..8399
                     /gene="srlA"
                     /locus_tag="ACHK74_003306"
                     /gene_synonym="gutA"
     CDS             7836..8399
                     /gene="srlA"
                     /locus_tag="ACHK74_003306"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFP0222068.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8396..9355
                     /gene="srlE"
                     /locus_tag="ACHK74_003307"
                     /gene_synonym="gutE"
     CDS             8396..9355
                     /gene="srlE"
                     /locus_tag="ACHK74_003307"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFP0222069.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9366..9737
                     /gene="srlB"
                     /locus_tag="ACHK74_003308"
                     /gene_synonym="gutB"
     CDS             9366..9737
                     /gene="srlB"
                     /locus_tag="ACHK74_003308"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP0222070.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGELHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9741..10520
                     /gene="srlD"
                     /locus_tag="ACHK74_003309"
     CDS             9741..10520
                     /gene="srlD"
                     /locus_tag="ACHK74_003309"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFP0222071.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10625..10984
                     /gene="gutM"
                     /locus_tag="ACHK74_003310"
     CDS             10625..10984
                     /gene="gutM"
                     /locus_tag="ACHK74_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFP0222072.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            11051..11824
                     /gene="srlR"
                     /locus_tag="ACHK74_003311"
                     /gene_synonym="gutR"
     CDS             11051..11824
                     /gene="srlR"
                     /locus_tag="ACHK74_003311"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFP0222073.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11817..12781
                     /gene="gutQ"
                     /locus_tag="ACHK74_003312"
                     /pseudo
     CDS             11817..12781
                     /gene="gutQ"
                     /locus_tag="ACHK74_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
     gene            complement(12778..14292)
                     /gene="norR"
                     /locus_tag="ACHK74_003313"
     CDS             complement(12778..14292)
                     /gene="norR"
                     /locus_tag="ACHK74_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFP0222074.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14479..15918
                     /gene="norV"
                     /locus_tag="ACHK74_003314"
     CDS             14479..15918
                     /gene="norV"
                     /locus_tag="ACHK74_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFP0222075.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            15915..17048
                     /gene="norW"
                     /locus_tag="ACHK74_003315"
     CDS             15915..17048
                     /gene="norW"
                     /locus_tag="ACHK74_003315"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFP0222076.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(17274..19526)
                     /gene="hypF"
                     /locus_tag="ACHK74_003316"
     CDS             complement(17274..19526)
                     /gene="hypF"
                     /locus_tag="ACHK74_003316"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFP0222077.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAATQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19679..20206)
                     /gene="hydN"
                     /locus_tag="ACHK74_003317"
     CDS             complement(19679..20206)
                     /gene="hydN"
                     /locus_tag="ACHK74_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFP0222078.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20355..21365)
                     /gene="ascG"
                     /locus_tag="ACHK74_003318"
     CDS             complement(20355..21365)
                     /gene="ascG"
                     /locus_tag="ACHK74_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFP0222079.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21625..23082
                     /gene="ascF"
                     /locus_tag="ACHK74_003319"
     CDS             21625..23082
                     /gene="ascF"
                     /locus_tag="ACHK74_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFP0222080.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            23091..24515
                     /gene="ascB"
                     /locus_tag="ACHK74_003320"
     CDS             23091..24515
                     /gene="ascB"
                     /locus_tag="ACHK74_003320"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFP0222081.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVELFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
BASE COUNT         5765 a         6762 c         6310 g         5734 t
ORIGIN      
        1 cgctactaaa gccatatcaa gtaagagatg gtgcatccgg gaggattcga acctccgacc
       61 gctcggttcg tagccgagta ctctatccag ctgagctacg gatgcatcgg gaaacttact
      121 ttactgcaga ttttttgata ccgctactaa agccgtatca agtaagagat ggtgcatccg
      181 ggaggattac tcgccttgcg gctcgccctt cgggccgttg ctaaagcaac gttatcctcc
      241 ctggtgcctg cggttatacc gccacctctt ccataccttc atcgctgaaa gtacgtagaa
      301 tatggtgcat ccgggaggat tcgaacctcc gaccgctcgg ttcgtagccg agtactctat
      361 ccagctgagc tacggatgca aatggcggtg aggcggggat tcgaaccccg gatgcagctt
      421 ttgaccgcat actcccttag caggggagcg ccttcagcct ctcggccacc tcaccacacg
      481 cctcttacga gtgcttcgaa gaacttgttt atcgctcatc gtcgctgcgt ggcgcacata
      541 ttactttctg agacttataa gtcaaacaat ttttcctaca cttttatcgt ttgcacactt
      601 cacgttcaat tagtctgtaa aaacgtcaaa aagagtgttt tatcaacaga aaaatggagg
      661 tctgacagat agtagtaatg caaaaaaatg gagacttaag ttgaatgaac gggagtaaag
      721 cgaaaagact atagagtgaa ggagaaattt tgagggtgcg tctcaccgat aaagatgaga
      781 cgcggaaaga ttagtaactg gactgctggg atttttcagc ctggatacgc tggtagatct
      841 cttcacggtg aacagaaact tccttcgggg catttacgcc aatacgtacc tggttgccct
      901 ttacccctaa aactgtcacg gtgacctcat ccccaatcat gagggtctca ccaactcgac
      961 gagtcagaat cagcattctt tgctccttga aagattaaaa gagtcgggtc tctctgtatc
     1021 ccggcattat ccatcatata acgccaaaaa gtaagcaatg acaaacacat tacatgtaag
     1081 cagtcatggc attacattct gttaaaccta agtttagccg atatacacaa cttcaacctg
     1141 actttatcgt tgtcgatagc gttaatgcga atgccgtgaa gcgagtccac ggcattgcct
     1201 gacgcttata ttattgcaat ttcgcgctga cccagccttt cacactggct aacgctgcag
     1261 gtaaggccgc agcatccgta ccaccggctt gcgccatgtc aggacgtcca ccacccttgc
     1321 cgcccacctg ctgagcgacc ataccaatca gttcccctgc tttcacacga tctgtgacgt
     1381 ccttagatac gcctgcaatc agagaaacct taccttcggc taccgttgcc agcacgataa
     1441 ttgtcgaccc cagctgattt tttaaatcgt caaccatggt acgcaacatt ttcggctcaa
     1501 caccgctaag ctcgctaacc aacagcttaa caccattaac atcaattgcc ttactggaaa
     1561 gatttgcgct ctcctgtgcg gcagcttgtt ctttaagctg ttgtagttct ttttctaact
     1621 gacgcgtacg ttccagtact gagcgcactt tatccgccag attattgcta tcgcctttca
     1681 gcagatgcgc gacttcgctt aagcgatcgc tgtctgcatg aacggtggtg atagcgcctt
     1741 ctccggttac cgcttcgata cgacgaacgc ctgcagcagt acccgattca gagatgatgc
     1801 ggaacagacc aatatcacca gtgcggctgg cgtgagtacc gccacacagc tcggtggaga
     1861 aatcgcccat gctcagcacg cgtacacgct catcatactt ctcgccgaac agcgccatcg
     1921 cacctttcgc tttcgccgct tcgagatcca tgatgttggt ttcgatcggc aaattgcgac
     1981 gaatctgtgc gttcaccagg tcttcgaccg cacgaatctc ttccggtttc atcgcttcgt
     2041 tgtgtgagaa gtcgaagcgc agcaccttgt cgttaaccag tgaacctttc tgcgatacat
     2101 gagtccccag aacctggcgc agcgcagcgt gcatcaggtg cgttgcggag tgattcagac
     2161 gaatacgggc gcgacgagcc tcatcaacat cagcctgcac agcgtcgccc actttcagag
     2221 aacccgcagc aagtttaccg atgtgaccaa tcgcctggcc gtatttctgc gtatcttcca
     2281 ccgcgaagga gaagttagcg cctttcagtt cgcctttatc accaacctga ccgccggatt
     2341 ccgcatagaa tggcgtttga tccagcacga ccacagcttc ctggcctgca ttgatggcat
     2401 caaccgcttt accatcaaca aacagcgcgg tcactttgcc gttcagttcc agatggtcat
     2461 agcctttaaa ttcagatgca ctatcaacac ggatcattgc gttgtaatcg gcaccaaagc
     2521 cgctggcttc gcgcgcacga cgacgctgct cttccattgc tgcttcaaaa ccagcttcgt
     2581 caactttgat gttgcgctca cgacaaacat cagccgtcag gtcaaccggg aagccatagg
     2641 tgtcgtacag acggaaagcc gtttcaccat ccagcgtatc accagaaagt tttgccagct
     2701 cttcatccag caacgccaga ccgcgctcca gagtacgggc aaactgctct tcttcagtct
     2761 tcagcacctg ctcaacctgc gcctgctggc gtttcaggtc ttcacccgca gagcccataa
     2821 cgtcgatcag cggaccaacc agtttgtaga agaaggtttc tttcgcgccg agcatattgc
     2881 cgtgacgcac tgcgcgacga atgatacgac gcagtacata accacggttt tcattggacg
     2941 gcattacgcc atccgcgatc aggaacgcac aagaacgaat gtggtcagcg attacgcgca
     3001 gcgatttatt gctcagatcg gtcgcgccag tgactttcgc taccgcctgg atcaacgtgc
     3061 ggaacaggtc gatgtcatag ttagagttaa cgtgttgcag caccgcagca atacgctcca
     3121 gacccatacc ggtatctaca gacggcttcg gcagcggttc catcgtgcca tctgcctggc
     3181 ggttgaactg catgaagacg atgttccaga tctcaatgta gcggtcgccg tcttcttccg
     3241 gacttcccgg agggccaccc caaatgtggt cgccgtgatc gtagaagatt tcggtgcacg
     3301 ggccgcacgg accagtgtca cccatctgcc agaagttgtc agatgcgtat ggcgcacctt
     3361 tgttatcgcc gatgcgaata atacgttcgc gcgggatccc tacttctttt tcccagattt
     3421 cgtaggcttc gtcgtcgctt tcatagacgg taacccacag acgctctttc ggcagggcaa
     3481 accatttttc gctggtcagc agttcccatg caaactgaat ggcatcgtgt ttgaaatagt
     3541 cgccgaagct gaagttgccc agcatttcga agaaggtatg gtgacgcgcg gtgtaaccga
     3601 cgttttccag gtcgttgtgt ttaccacccg cacgcacgca gcgttgggaa gtggtagcgc
     3661 gggaataatt acgcttgtcg agcccaagga acacatcctt gaactggttc atcccggcgt
     3721 tggtaaacaa caaagttggg tcgttatggg gtaccaggga gctgctggca actacctgat
     3781 gtcccttact atggaaaaag tcgagaaacg cctgacggat ctcagcggtg ctcttgctca
     3841 taattatcct gaaatcaagc taacgaaata tcgccaccag ctccagcgtg tcttaaccgc
     3901 cgggctggta actgaaaagt gggaataaga taagttttct tgactgggaa gtaaaatacc
     3961 gtatgcgttc agtcggcaaa atttcgccaa atatcctgga tatcttccat cagatagcca
     4021 cgatagagca aaaaacgctg gatcttaact ttttctgaaa agacagttgg caaaggttca
     4081 ccatattttc gcgtcgcctg atcgcgcgcc agtgcgcacc agtcgatgtc acattcacgc
     4141 atcgcttttt ctgtcgcttc gcgggaaata cctttctgat tcagttcctg gcgaatacgc
     4201 gcaggtccat aacctttgcg gctacggctg gcgataaagc gcgcaacaaa tcggctgtca
     4261 tcgagatagc catgttcatg gcaccaggca ataacgcgct cgtaatcttc tgccgtagca
     4321 tcaatctctt ctgggccatt tttgcccata atcggtgccg cgagtttacg tcgcagttct
     4381 tgctcactgt gatcgcgcac cgccagaatg cgtaccgcac gatccaacag gcgagcatat
     4441 gccgggcgac gggacgttga ttctgtcatg gcatatcctt acaacttaaa aaagcaaaag
     4501 ggccgcagat gcgacccagg ttttgctgat aaacagattt aatagtcaat gagtgtgcag
     4561 aatgttcccg caggcccaat tcgttagagg atcgaggtga gagtaatttt tcacgccagt
     4621 gctggcctgt tcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgtccgtgca
     4681 cacattatct attatcatca ataacgaacg attaaaaatc ttcgttagtt tctgctacgc
     4741 cttcgctgtc atctacagag aaatccggcg ttgagttcgg gttgctcagc agcaactcac
     4801 gtactttctt ctcgatctct ttcgcggttt ccgggttatc tttcagccag gcagttgcat
     4861 tcgctttacc ctgaccgatc ttctcacctt tgtagctgta ccacgcgcct gctttctcga
     4921 tcagcttctc ttttacgccc aggtcaacca gttcgccgta gaagttgata ccttcgccgt
     4981 agaggatctg aaattcagcc tgtttaaacg gtgcagcgat tttgttcttc accactttca
     5041 cgcgggtttc gctacccacc acgttttcgc cctctttcac cgcgccgata cgacggatgt
     5101 cgagacgaac agaggcgtag aatttcagcg cgttaccacc ggtagtggtt tccgggttac
     5161 cgaacatcac accaattttc atacggatct ggttgatgaa gatcagcagc gtgttggact
     5221 gcttcaggtt acccgccagc ttacgcatcg cctggctcat catacgtgcc gcaaggccca
     5281 tgtgagagtc gccgatttcg ccttcgattt ccgctttcgg cgtcagtgcc gccacggagt
     5341 caacgacgat aacgtctact gcgccagaac gcgccagggc gtcacagatt tccagtgcct
     5401 gctcgccggt gtccggctgg gagcacagca ggttatcgat atcgacgccc agtttacgtg
     5461 cgtagattgg gtccagcgcg tgttcagcat cgataaacgc acaggtttta ccttcacgct
     5521 gcgctgcggc gatcacctgc agcgtcagcg tggttttacc ggaagattcc ggtccgtaga
     5581 tttcgacgat acggcccatc ggcagaccac ctgccccaag cgcgatatcc agtgaaagcg
     5641 aaccggtaga gatggtttcc acatccatgg aacggtcttc acccaggcgc atgatggagc
     5701 ctttaccaaa ttgtttctca atctggccca gtgctgccgc caacgctttc tgtttgtttt
     5761 cgtcgatagc catttttact cctgtcatgc cgggtaatac cggatagtca atatgttctg
     5821 ttgaagcaat tatactgtat gctcatacag tatcaagtgt tttgtagaaa ttgttgccac
     5881 aaggtctgca atgcatacgc agtagcctga cgacgcaccg catcacggtc accgctgaag
     5941 cattcccgcc gggtaatgcc ttcaccgcgg gcagtggcaa aagcaaacca gacggtgccg
     6001 acaggcttct cttcactgcc gccatccggc ccggcgatac cactaataga cacggcataa
     6061 tcagcacgag ccgctttcag tgcacctatc gccatttcca ccacgacggg ttcactcacc
     6121 gcgccatgct gcgccagcgt ctcttcgcgt acgccgatca tctgcgcttt ggcttcgtta
     6181 ctgtaggtga caaatccgcg ttcaaaccag gcggagctac cggcaatatc ggtaatcact
     6241 ttcgctaccc aaccaccggt acaagactcg gcagttgtta cggttgcgcc acgggctttc
     6301 agcgcctgcc caacctgttc acttaactgc atcagttcac tgtcagtcat cacaacctct
     6361 gtcagtcaaa aatttatgcc ggacaagata gcactttcgt caggaagatg gtgttggcgt
     6421 tgcaatttta cgaggaggat tcagaaaaaa gctgattagc cagagggaag ctcacgcccc
     6481 cctcttgcaa atagttactg cacgcgcgcc agcgccacgg cttgtcctaa ctgccagacc
     6541 gccattgcgt aatgggtgct gtggttataa cgggtaatgg tgtagaagtt cggcagaccg
     6601 taccagtact ggtaaccggt accgacatcc agacgcagca ggctggcttg ttgatggttg
     6661 cccagcggct gctgtggcgt taaacctgcc gtggcaagct gcgaaatgct gtacctggtt
     6721 ttgaagccat ttggcaagcc tggagcctga ccgtttgcca ttaccgcgac ctgatcgcct
     6781 ttcacccagc cgtgcgcttt gaaatagttc gccacgctac cgatcgcatc aaccggatcc
     6841 cacaggttga tatgcccgtc gccgctgaaa tctaccgcat attgtttgta agacgacggc
     6901 ataaactgtc cgtagcccat cgccccggca aaggaacctt tcagattgag cggatcgtcc
     6961 tgctcgtcgc gcgccatcaa caggaaggtt tccagctcgc cagagaaata ctccgcgcgg
     7021 cgtgggtagt taaatgacag cgttgccagc gcatcgagga tgcgagtttt ccccatcacg
     7081 cgcccccagc gggtttcaac gccgataatc ccgacgataa tttccggcgg tacgccatac
     7141 acctgccacg cgcgattcaa cgcatcttca tactgattcc agaaaaccac accgttctgc
     7201 acgttgtccg gcgtaataaa ttttttgcga taacggagcc atgcgccgtt cggacctgat
     7261 ggcggtttca ccgatgtggt tggtgcctgg ttatccatca gccgcagtac cgaatccaga
     7321 cgcttcgcct gggagagaat ttcctgcaac tgctgacgat cgaaaccgtg tttgttcacc
     7381 attttgtcga tgaactgctg ggcattcggg ttattagcga aatcgccgcc catctgcatc
     7441 acattgtgct gcggctcaag caggaagccg ccagacggcg ttccggtggt cgtttcagtc
     7501 tcagtaggtt ttggcttgct gctacaggcg gcaagcaaca caaaaagggg aagcaatgtt
     7561 acataacgac gcttgaacat gaggggtcca tttaacagat tcaaccaggg gcaagtatgg
     7621 taaagcatca cgccccacac aaggaagcgg tagtcactgc ccgatacgga ctttacataa
     7681 ctcaactcat tcccctcgct atccttttac tcaaactttc aaattaaaat atttatcttt
     7741 cattttgaga tcaaaataac acttttaaat ctttcaatct gattagatta ggttgccgtt
     7801 tggtaataaa acaataaatc ctgaaggaga gaacaatgat agaaaccatt actcatggtg
     7861 cagagtggtt tatcgggctg ttccaaaagg gcggagaggt gtttaccggg atggtgaccg
     7921 gcattcttcc gctgttgatt agcctgctgg ttatcatgaa cgcactgatt aattttatcg
     7981 gtcagcatcg tattgaacgt tttgctcaac gttgcgccgg taaccctgtt tcccgttacc
     8041 tactgttacc gtgcattggc acgtttgtct tttgcaatcc gatgacctta agcctgggtc
     8101 gctttatgcc ggaaaagtac aaacccagct actacgcggc ggcctcttat agctgccact
     8161 caatgaatgg cctcttcccc catatcaacc ctggcgaact gtttgtttat cttggcattg
     8221 ccagcggtct gacaacgctg aacctgccac ttggcccact ggcggtgagt tatctgctgg
     8281 ttggtctggt caccaatttc ttccgcggct gggtgaccga tctgaccacc gccatttttg
     8341 agaaaaagat gggcattcaa cttgaacaaa aagttcacct ggcaggagca acatcatgac
     8401 gcatattcgg atcgaaaaag gaacgggtgg ctggggcggc ccgcttgagc tgaaagccac
     8461 gccgggaaaa aaaatcgtct atatcaccgc cggtacccgg cctgcgattg ttgacaaact
     8521 ggcacagctt actggctggc aggctattga cggatttaaa gaaggtgaac ccgcggaggc
     8581 ggaaattggt gtcgcggtaa tcgactgtgg cggcacatta cgctgcggca tctatccgaa
     8641 acgacgtatt cccaccatta atatccactc gacgggcaag tccggtccgc tggcgcagta
     8701 cattgtggaa gatatttatg tctctggcgt aaaagaagaa aacatcactg tagtgggtga
     8761 tgcgacacca caaccctctt ccgtgggccg tgactatgac accagtaaga aaatcaccga
     8821 acaaagcgat ggtttactgg cgaaggtggg aatgggcatg gggtccgccg ttgcggtgct
     8881 gtttcaatct ggtcgtgaca ccatcgacac tgtattaaaa accattctgc cgtttatggc
     8941 attcgtctcg gcgctcattg gcatcattat ggcttctggc cttggtgact ggattgccca
     9001 cggtcttgct ccgctggcga gccatccact gggtctggtc atgctggcgc tcatctgctc
     9061 cttcccactg ctttcacctt tcctcggccc aggcgcagtt atcgcacagg ttatcggcgt
     9121 attgattggc gtgcagattg gtctcggcaa tattccgccg catctggctt taccggcact
     9181 gtttgccatc aacgcgcagg cggcctgcga cttcatcccg gtcggtttgt cgctggcgga
     9241 agcccgtcag gacacggttc gcgtcggtgt cccttctgta ctggtgagcc gctttttaac
     9301 cggcgcgccg actgtactga tcgcctggtt tgtctccggt tttatctatc aatagaggct
     9361 gaaacatgac cgttatttat cagaccacca tcacccgtat cggcgcgagt gccactgacg
     9421 ccctcagcga ccagatgctc atcacctttc gtgaaggcgc gccagcggac ctcgaagagt
     9481 attgcttcat tcattgccac ggcgagttga aaggtgaact ccatcctggt ttgcaattta
     9541 cgctcgggca gcatcgctat ccggtgaccg ccgttggcag cgtggcggaa gacaaccttc
     9601 gcgaactggg tcatgtcacc ctgcgcttcg atggtttaaa cgaagcggaa tttccgggca
     9661 ctgtccatgt ggcaggccct gtacccgacg atatcgcgcc gggatcggtt ttgaagtttg
     9721 aatctgttaa ggagtaaaaa atgaatcagg ttgccgttgt catcggtggt gggcaaacct
     9781 taggcgcgtt cctgtgccac ggtctggctg ccgaggggta tcgcgtcgcg gttgtcgata
     9841 ttcagagcga caaagccgca aatgtggcac aagaaattaa cgccgaatat ggtgaaagta
     9901 tggcgtacgg ttttggtgct gacgccacta gcgagcaaag cgttctggcg ctctctcgtg
     9961 gggtagatga aatctttggt cgcgtggatt tgctggtcta tagcgccgga atagcaaaag
    10021 cagcctttat cagcgacttc cagctcggcg attttgaccg ttcgctacaa gtgaatctgg
    10081 tgggttattt cctgtgtgcc cgtgaatttt cgcgtttgat gatccgcgac gggattcagg
    10141 gtcgcattat tcagatcaac tcgaaatccg gcaaagtggg cagcaaacac aactctggct
    10201 acagcgcagc gaaatttggt ggcgtcgggc tgactcaatc actggcgctg gatctggcgg
    10261 agtacggcat tacggtgcat tcactgatgc tcggtaacct gctgaaatcg ccgatgttcc
    10321 agtcactgtt gccacaatac gcgaccaagc tgggtatcaa accggatcaa gtcgagcagt
    10381 attacatcga caaagtaccg ctcaaacgcg gctgcgatta tcaagatgtg ctgaatatgc
    10441 tgctgttcta cgccagtcct aaggcgtcgt actgcaccgg acagtcgatc aatgtcaccg
    10501 gcggtcaggt gatgttctga tcaacagcgg agatccatta aggatctccg tgagactata
    10561 gaatgcctga tgcgctacgc tcatcaggca tacaggactt ccgccactac attaaggaaa
    10621 agttatggta tccgcactca tcaccgtcgc cgttatcgcc tggtgtgcgc aactggcctt
    10681 aggcggctgg caaatttctc gttttaaccg tgccttcgac acactatgcc agcaagggcg
    10741 ggttggcgtg ggccgttcca gcgggcgctt taaaccacgg gtcgtggtcg ccatcgcgct
    10801 ggacgatcag cagcgcatcg tcgacacctt gtttatgaaa ggactgaccg tcttcgcccg
    10861 accgcaaaaa attcccgcaa ttaccggtat gcatgcgggt gatttacagc ccgatgtgat
    10921 ctttccccat gatccactat cacagaatgc tctatcattg gcgcttaaac tgaaacgtgg
    10981 ataatttcgt tgtgaatgtt acttgcttgc gaagttatca ttttgaaacc taaatcaggt
    11041 aatcacgccc atgaaacctc gtcagcgtca ggccgccatt ctggagtatc tgcaaaagca
    11101 gggtaaatgc tcggttgaag aattggcgca atactttgac accacaggca caaccattcg
    11161 caaagatctg gtcattctgg aacatgccgg aaccgtcatt cgtacttatg gcggagtggt
    11221 gttgaataaa gaggaatccg atccgcctat cgatcataaa acactcatca acacccacaa
    11281 gaaagagctg attgcagaag ctgccgttag ttttatccat gatggcgatt cgatcattct
    11341 tgatgctggc agtaccgttt tgcagatggt tcccctgctc tcgcgcttta ataacatcac
    11401 ggtgatgacc aacagcctgc atatcgtcaa tgcgctatcc gaactggata acgaacaaac
    11461 tatcctgatg ccaggcggaa cgtttcgtaa aaaatcggcc tcatttcacg gacagctggc
    11521 agagaatgcc ttcgaacatt tcagtttcga taaattgttt atgggcaccg acggcatcga
    11581 tctcaatgcg ggcgtaacca ccttcaacga ggtttatacc gtcagtaagg caatgtgcaa
    11641 tgccgcgcgc gaagtgattt tgatggcgga ctcatcaaag tttggccgta aaagccccaa
    11701 cgtagtttgc agtcttgaaa gcgtcgataa gctgattacc gacgcaggta tcgatccggc
    11761 gtttcgtcag gcgctggaag agaaagggat cgatgtgatc ataaccggag agagcaatga
    11821 gtgaagcact actgaacgcg ggacgtcaga cgttaatgct ggagttgcag gaagcaagcc
    11881 gtttaccgga acgtctgggc gatgattttg ttcgcgccgc caatatcatc ctgcactgcg
    11941 aaggcaaagt ggtggtttcg ggaattggca aatcgggcca cattggtaag aaaatcgccg
    12001 caacacttgc cagtaccggc acaccggctt ttttgtccat ccggcagaag cactgcacgg
    12061 cgatctgggg atgattgaaa gccgcgatgt gatgctgttt atctcttact ccggtggcgc
    12121 gaaggaactg gatctgatta ttccgcgtct ggaagataaa tctatcgcgc tgctggcgat
    12181 gaccggcaaa ccgacgtcac cgctgggcct ggcggcaaaa gcggtgctgg atatctccgt
    12241 agaacgcgaa gcctgcccga tgcaccttgc gccgacctcc agcaccgtca ataccctgat
    12301 gatgggtgac gcgctggcga tggcggtcat gcagacgcgc ggatttaatg aagaagattt
    12361 tgcccgctcc cacccagccg gggcactggg cgctcgcttg ctgaataaag tgcatcatct
    12421 gatgcgccgt gacgatgcca tcccacaggt ggcgttaacc gccagcgtga tggatgcgat
    12481 gctggaactc agccgcaccg gtctggggct ggtggcggta tgtgacgctc aacaacaggt
    12541 acaaggcgtc tttaccgacg gcgatttacg tcgctggctg gttggcggcg gcgcactcac
    12601 cacgccagtt aatgaagcga tgacgacagg cggcaccaca ttacaggcac aaagtcgcgc
    12661 catcgacgcc aaagagatcc tgatgaagcg caaaatcact gccgcaccgg tggtggatga
    12721 aaacggcaaa ctcaccggcg caattaacct gcaggatttc tatcaggccg ggattattta
    12781 atccttcaat cccagacgtt tcgccagccg atgcaggttg gcgacgtcgg tttccagcat
    12841 ccgcgcgcag gcagcccagt tgtgatgatt ttgtgccagt gcctggcgaa tggtttcacg
    12901 ctggaacgct tctgtcgctt cacgcaggtt ttgcttaaca acgggcaccg ccgccgcttc
    12961 tggcggcggc aacgtcacct caggaaaagc aaaatgttgt gcctcaagaa tcacttcatc
    13021 gccgctgcga gtggctctcg ccagcactac cgcccgatga atagcatgtt ccagttcgcg
    13081 cacgttcccc ggaaaacgat agtgttgcag taaatttcgc gctccggcac ttaataccac
    13141 gcgggagagc cccagccgca aacgacactg ctcgcagaaa taccccgcca gcagaatgac
    13201 atcatcgccc cgctcacgca gcggcggcac cgaaagtgga aacacgctca ggcgatgaaa
    13261 caaatcggcg cggaatcgcc ctgccagcac ctcttcgcgt aagtcgcggt tagtcgccgc
    13321 cagcacgcgc acatcgaccc gcaaactacg gtcatcgcca acgcgctgaa tatcgccata
    13381 ctgcaacacc ctcagcagct tggcctgcaa tgccaacgac aactcgccga tctcatcgag
    13441 aaacagagtg ccgttatccg ccatttcaaa cttcccgctg cggttactga tagcgccagt
    13501 aaacgctcct ttcacatgcc cgaacaactc actttccgcc acactttccg gcagtgcggc
    13561 acagttgaga tagaccagcg gattcaccgc ccgtggcgag gcttcatgaa tcgctttcgc
    13621 caccagctcc ttaccggttc ccgtctcacc gctgatcaga acgttgagat cggacgccgc
    13681 cacaatctca atctcttttt tcaattgcgt catgcctggc gacaagccaa tcatctgcgt
    13741 ctgtttcacc gcttcaaacg gcgcggcatc gcccggcagc atattctggc tttccagttg
    13801 ttcaatcagc aacgcattgc ttaacgctcc cgccgccagc gcggcaatca gccgtaactc
    13861 ttcgtcgctg aaaacatcga actgatcggg ctgcatcccg tcgagcgtca gtgcgccgat
    13921 caggttttgc ccggcaaaca atggcagacc aacgcaggcg tgaaccttca gactctcctg
    13981 cccaggaatc aaaccgtcat agggatcggg caattcgctg tctgcaggaa agcgcaccac
    14041 gtccccggcg cgggcaatcg cttcaagccg tggatgccct tccagcgcaa agcgtctacc
    14101 gagtacatcc tttgccagac cgtcgatggc aagcggaata aactgccgcg aatcgtaacg
    14161 tagcaacgca gacgcatcgc actccagcac ctgacgtagc gtggtgatca ggcgctgaaa
    14221 acgatcctga tgaccaatcc cacgctgcaa ttcgatggcg atattcgcca gcacatcaac
    14281 ggaaaaactc atctttgcct cactgtcaat ttgactatag atattgtcat atcgaccatt
    14341 tgattgatag tcattttgac tactcattaa tgagcataat tttatttata gagtaaaaac
    14401 aatcagataa aaaactggca cgtaatctgc aattagcaag acatcttttt agaacacgct
    14461 gaataaattg aggttgctat gtctattgtg gtgaaaaata acattcattg ggttggtcaa
    14521 cgtgactggg aagtgcgtga ttttcacggt acggaatata aaacgctgcg cggcagcagt
    14581 tacaacagct acctcatccg cgaagaaaaa aacgtgctga tcgacaccgt cgaccataaa
    14641 ttcagccgcg aatttgtgca gaacttgcgt aatgaaatcg atctggcgga tatcgattac
    14701 atcgtgatta accatgcaga agaggaccac gctggggcgc tgaccgaact gatggcgcaa
    14761 attcccgata cgccgattta ctgtactgcc aacgctatcg actcgataaa tggtcatcac
    14821 catcatccgg agtggaattt taatgtggtg aaaactggtg acacgctgga tatcggcaac
    14881 ggcaaacagc tcatttttgt cgaaacgcca atgctgcact ggccggacag catgatgact
    14941 tacctgacag gcgacgcggt gctgttcagt aacgatgctt tcggtcagca ctactgcgac
    15001 gagcatctgt tcaacgatga agtggatcag acggagcttt tcgagcagtg ccagcgttac
    15061 tacgccaata tcctgacgcc gttcagccgc ctggtaacac cgaaaattac cgagatcctg
    15121 ggctttaact taccagtcga tatgatagcg acctctcacg gcgtggtatg gcgcgataac
    15181 ccgacgcaaa ttgtcgagct gtacctgaag tgggccgctg attatcagga agacagaatc
    15241 accattttct acgacaccat gtcgaataac acccgcatga tggctgacgc tatcgcccag
    15301 ggaattgcgg aaaccgaccc acgcgtggcg gtgaaaattt tcaacgtcgc ccgaagcgat
    15361 aaaaacgaaa tcctgacaaa tgtcttccgc tcaaaaggcg tgctggtcgg cacttcgacg
    15421 atgaataacg tgatgatgcc gaaaatcgcc gggctggtgg aggagatgac tggtttacgc
    15481 ttccgtaaca aacgcgccag tgctttcggc tctcacggct ggagcggcgg tgcggtggat
    15541 cgtctttcca cgcgcctgca ggatgcgggt ttcgaaatgt cgcttagcct gaaagccaaa
    15601 tggcgaccag accaggacgc tctggagtta tgtcgtgaac acggtcgcga aatcgcccgt
    15661 cagtgggcgc tcgcgccgct gccgcagagc acggtgaata cggtggttaa agaagaaacc
    15721 tctgccacca cgacggctga cctcggccca cggatgcagt gcagcgtctg ccagtggatt
    15781 tacgatccgg caaaaggcga gccaatgcag gacgttgcgc caggaacgcc gtggagtgaa
    15841 gtcccggata acttcctctg cccggaatgc tccctcggca aagacgtctt tgaagaactg
    15901 gcatcggagg caaaatgagt aacggcattg tgatcatcgg ttcgggcttc gccgcccgcc
    15961 aactggtgaa aaatattcgc aaacaggacg ccactattcc attaaccctg attgccgccg
    16021 acagcatgga tgagtacaac aaacctgacc tcagccatgt tatcagtcag gggcaacgtg
    16081 ccgatgacct tacccgccag acggcgggtg aatttgccga gcagtttaat ctgcacctgt
    16141 ttccacaaac ctgggtgacg gatatcgatg ccgaagcccg tgtggtgaaa agccagaata
    16201 atcagtggca atacgacaag ctagtactgg caaccggtgc cagtgccttt gtcccgcctg
    16261 tgcctgggcg tgagttaatg ctgacgttaa atagtcagca agagtatcgc gcctgtgaaa
    16321 cgcaactgcg ggatgcccga cgcgtgttga ttgttggcgg tggtttgatt ggtagcgaac
    16381 tggcgatgga tttttgtcgt gcaggcaaag cggtcacgct aatcgacaac gctgccagta
    16441 ttctggcgtc attaatgcca ccggaagtaa gcagccgctt gcagcatcgg ttgacggaga
    16501 tgggcgttca tctgttgttg aaatctcagt tgcaggggct ggaaaaaaca gattctggca
    16561 ttctggcaac gctggaccgc cagcgcagta tcgaagtgga tgcggtaatt gccgccaccg
    16621 gactgcgccc ggaaaccgcc ctggcacgac gcgccgggct gacgattaat cgcggcgttt
    16681 gcgtcgatag ttatctgcaa accagtaatg ccgatattta cgcgctgggc gattgcgcgg
    16741 aaattaacgg tcaggtattg ccgttcctcc agccgattca acttagcgcg atggtgctgg
    16801 caaaaaatct tctcggcaat aacacgccac tgaaactacc ggcgatgctg gtgaaaatca
    16861 aaacgccgga attaccgctg catctggcag gcgaaaccca gcgtcaggat ttacgctggc
    16921 aaattaatac cgaacgccag ggaatggtgg cgcgcggcgt tgacgatgct gaccagcttc
    16981 gcgcctttgt ggtcagtgag gatcggatga aagaggcatt tggattgttg aaaacattgc
    17041 cgatgtaggt gggctactgt gcctaaaatg tcggatgcga cgctggcgcc tcttatccaa
    17101 cctacgggga gcgcatgtgt tggtcacata ctgcgttcat gtcgcatcca cctactgtgc
    17161 ccaaaatgtc ggatgcgacg ctggcgcgtc ttatccgacc tacggggacg catgtgtagg
    17221 ccggataagg cgtttacgcc gcatccggca atggtgtcca aatgcaacac gttttatccg
    17281 ttctggactt cacccgctaa ccaacgcgcc gcagcaataa ccccctgccc cagagacaaa
    17341 ccgccatcac ccgccggtaa actctgtgga aagagcaatg tgaaatcagc gagataatgc
    17401 gccagacgtg cacgcagcaa acggttatga ataaccccgc cgctaaatac cagcgtagtg
    17461 ataccacgca tcgtggcctg ctcacgcatc aacgcggcaa aaccctgcgc cagcgcatca
    17521 tgaaacgccc acgcgcgttg attaaccggt gcctgccagt tcagccactg ctgccagaaa
    17581 gtggcgagat ccagttgatt gtccaccagc ggcattgtca ccggatgcgt cactccgtgg
    17641 cacgaagctg cgagtgcctc cagagcacaa gccgcttcac cttcataact taacgtggct
    17701 ggcgcacagc ccagtgccgc cgccacggca tcgaacaacc gcccacacga tgacgccagc
    17761 ggcgcgttaa ttccacgctc aatggcccgc gccagcacgc tccagttttg ctgttgcaca
    17821 cttgctgttt cagaataatt ctgccactcc ggcacaaagc gcaggcactg cgccagcagg
    17881 tttcgccacg gctgcgtcgc cgccaaatcg ccacccggaa gcgccactgc aggcaagccg
    17941 cccaggtgct cacattcgcg atagttcacc cgcagacact cgccgcccca caaagcgccg
    18001 ttctccccca taccgatacc atcgagcgtc aaagcaatga catcaccgcc atccagcggc
    18061 cactgatgct ctgccagaca cgccgctgcg tgggcatgat gatgcagcac cgtttgcgtc
    18121 ggcatattca tttcgctcgc ccactggctg gagacatagc ccggatgcac gtcatgcaca
    18181 acgtattgcg gagtgaaatc gtagatgttt tgcatcaggc gtaacgcttc gcgccactgc
    18241 atctggatgc catcgtcact taaatcgccc agatgctgac tcaacaccgc ttgttcaccg
    18301 cgcaccaggc agaaggtgtt tttcagatcc gcgccaagac acagcacagg cggaacattt
    18361 ttaaagcccg gaggcaaaac cagcgcatcc ggcacatacc cgcgtgaacg gcgcagcatt
    18421 tcgccgcttt cgcgcaccac cgaatcatcc atccgctgca cgatgtcgcg gttatgtatc
    18481 aagaatccgt cggcaatgcc ctgcaaatcc gccagcgcct gttcgttgct gatagctggt
    18541 ggtttaccgc tcaggttgcc ggaggtcatc accagcgggc attgcagttc ctgtaacagc
    18601 aaatgctgga gcgggttcgc aggcaacatt accccgactt cgttaaggtc aggggcgata
    18661 tcatcacaaa gctcaggaac gtatttttta tccaccagca caatcggcgc ggcgggcgtg
    18721 gtaagcaact ggcgcgcagc gtctggtaaa ccgtcagcca ctggcaacat gaccgccagc
    18781 ggtttcgccg ggcgatgttt gcgcgcccgc agtgtcgcca ccgcgttact gttacgtgca
    18841 tcgcaggcaa gatgaaatcc gccaatccct ttgatggcga caatgttgcc catttttaac
    18901 tgtgcgatag ccgcctgtaa tgccgcctct tgttctgcat gttcaccatg acttacccat
    18961 tcaagatgtg ggccacactc cgggcaggcc accggctggg cgtggaagcg acgatcgagc
    19021 gggtcgcggt actctttgtc acaagccgga cacaacggaa acgccgccat cacggtaaac
    19081 gggcggtcgt aaggcatggc gcgaataatg gtgaaacgcg ggccgcagtg ggtacagttg
    19141 ataaacggat aacgataacg ccgttcgcct ggggtattca tttcggcaag acaggcaggg
    19201 caagtagcgg catccgggac aatttgcgta ttcatggcac ctcccgcgct ctggcgtata
    19261 gtgaactcgg tgggcagttg tgaccagatg tacggctcac gctcgacgct atcaatacgc
    19321 gccagcggcg ggcagtgctg atgcaattga acaagaaacg tttccgggtc ttccagcagc
    19381 cggacttcta cgccatcgcc gtcattacag acatcgccgt gaagatttaa ttgctgtgcc
    19441 agctgccaga caaacggacg aaaaccgacg ccctgcactt tgccacgaat acgcagttgg
    19501 acaccgcaag atgtgttttt tgccattgag ttattcccgc catcatgaat tgcgtaaccc
    19561 gccctgccgg acacgactgc gtcgtatccg gcagtctcag gtcggcgata ccgccagctc
    19621 cgtattctac gaatatttcc gggaattcct ttgatgccag aacagttcta taagattttt
    19681 agaacatcag cgccgtacgg cgacgttttt ctgcgctcag ttgttcaagt ttattacgat
    19741 cgacacaaat cagcgcatgg gtcgggcaag ccgccataca cgccgggccg tcttcgcgat
    19801 ggttgcacag gtcgcattta ttggcttcag ctttgtcagc ccgtacattc agccccgcgc
    19861 cgctgttgcg gatcaccgga cgtaccacca cttccatcgc gccatacggg caagccacca
    19921 cgcaggtttt gcaaccaatg caacgttcct gcatcacatg aacaaaccct ttatcacggc
    19981 tgatagcacc attcgggcag acgttagcgc acggtgcatc ttcacactga cggcagactg
    20041 tcgccgtgga aatgttcaca cctttaatga catggattcg cggtaaaaag gtttccgggg
    20101 tcagcgatgc acagtcctga ttttcctgat gagaaaccac gcacgccact tcacaggtac
    20161 ggcaaccaat acatttactc gcgtcagcaa tgatgaaacg gttcatcaaa ttctccagca
    20221 atgacagtta atgcgccgat acattcacaa atcatgccag tttttaattc actgttattt
    20281 aaggaaatta atttctgtaa tgcaggaaaa aagatgtcat cgacactagt gacgatgaca
    20341 tgtgatgaca atgtttatcg cgaaagagca atgagtgagt cgcggcggat cagttttccg
    20401 ctgaaggttt tcggcggtga gaaatccccg ccatcgagca taaaaatcag ccgtccaata
    20461 atttcctgaa tcatctcagt caccggaatt tttacgctgg agagcgccgg aacgatatag
    20521 ggggcaattg cgatatcatc gaatccgata actgacacct gctctggcac cgctacgccg
    20581 cgctcgtgca acgctttcat cgcacctatc gccatatcgt cgttactggc aactaacgcg
    20641 ctaaatttag ccccacgttc gagcaacgtt tctacccctt cggccccgct ggcgggcgtc
    20701 catttaccgt tagcgataag tttttcattg agcgcaatac catgctgcgc cagcgcgtct
    20761 ttatacccag caagacgttc aatgctggtg ggggaatcca tcgagccggt aaggaaagca
    20821 atctcctgat gtccggcgtt tatcaactct gccacggcat taaagctggt ctgtttatga
    20881 tcgcaccaga cgctatggct gctatttttg cgcaggcggc gattaagcac cattatcggc
    20941 tgactgtgcg cgtcaataat gtcatcgatc tcatccacgc ttaaaaaacg cgggtaaatc
    21001 ataatggcgt cgcagcgcaa atccagcaga tactgaatcg cctggcgctc ttcttcggcg
    21061 ctgtgtttac catctgccaa tagcaactgc cgccctttct cttccgccat tcgcgcggca
    21121 tgaaagagta attcactaaa ataaatgccg tggtaaagcg tgttggtcac taccagcccc
    21181 agcgtctgag tactcttcgc cgacagattg cgcgccagca agtttgggcg gtaaccgctc
    21241 tcttctaccg cctgaaacac gcgatcttta gtctcctggc tgacgtagcc attacctgaa
    21301 agcacgcggg aaacggtcgc ttttgaaacc ccggcgcgct tcgccacttc cagcatcgtc
    21361 gtcatcattt tcatcccttt acacgcaatc aacgcagtgt actgcaccgt ttgccgattg
    21421 tccttgcgca atcggcggga aaaatattca ggtgaccggt ttcacaaata taaaaaatga
    21481 acaattaacc ctcttgctta tttagtgaca actattcatg attttgtgaa accggtttcc
    21541 taattccgtt tcagcatcgg catttttccg tcacgtcgac tgataacaac tacatctacc
    21601 ctactgataa caggataaaa tccgatggcc aaaaattatg cggcgctggc acgctcggtg
    21661 atagcggcac tgggcggcgt tgataacatc tcggcagtca cgcactgtat gacgcgtttg
    21721 cgctttgtta tcaaagatga ccagcttatc gacagcccga cgttaaaaac catccccggc
    21781 gtgctcggcg tggtacgtag tgacaaccag tgtcaggtga ttatcggcaa taccgtttca
    21841 caagcctttc aggaagtcgt cagcctgctg ccgggagata tgcagcccgc acagcccgta
    21901 ggtaaaccca aactcacgct acgtcgcatt ggtgcgggga tcctcgatgc gctgatcggc
    21961 accatgtcac cgctgatccc ggcgattatc ggcggatcga tggtcaaact gctggcgatg
    22021 atcctcgaga tgagcggcgt gctgacaaaa ggatcgccga ccttaactat tctgaatgtg
    22081 attggtgacg gtgctttctt cttcctgccg ctgatggtcg cggcttcggc cgccatcaaa
    22141 tttaaaacca atatgtcgct ggcgattgcc atcgcgggtg tgctggtaca tccgagcttt
    22201 attgaactga tggcgaaagc ggcccagggt gaacatgtcg aatttgccct gatcccggtc
    22261 accgcggtga aatacaccta cacagtgatc ccggcgctgg tgatgacctg gtgcctgtca
    22321 tatatcgaac gctgggtgga tcgcattaca ccggcggtga ccaaaaactt cctcaaacca
    22381 atgctgattg tgttgattgc cgcaccgctg gcaatcctgc tgattggccc gattggtatc
    22441 tggatcggta gcgccatttc ggcgctggtt tacaccattc atggttatct gggctggctt
    22501 tcagtcgcca ttatgggcgc gctgtggcct ctgctggtaa tgaccgggat gcaccgcgtc
    22561 tttacgccaa ccatcattca gaccattgcc gaaaccggca aagaagggat ggtcatgccg
    22621 tcagagatcg gcgctaacct gtcgctgggc ggttcatcac tggcggtggc gtggaaaacg
    22681 aaaaacccgg aactgcgcca gacggcgctg gctgcggcgg catcagccat tatggcgggg
    22741 atttccgaac cggcgttata cggcgtggcg atccgcctga aacgtccgct tatcgccagt
    22801 cttatcagcg gttttatttg cggtgcggtt gccggtatgg cggggcttgc cagccactca
    22861 atggcagcgc cggggctatt taccagcgtg cagttcttcg atccggcgaa tccaatgagc
    22921 atcgtctggg tgttcgcggt catggcgctg gcggtcgtgc tgtcgtttat cctcacactg
    22981 ttgctcggct ttgaggatat tcctgttgag gaagcggctg ccgaggcgcg aaagcatcag
    23041 agcgtacaac cgaccgtcgc caaagaagta agtcttaatt gaggatgaaa atgtcagtat
    23101 ttccagaagg ttttttatgg ggcggcgcgc ttgccgccaa ccagtctgaa ggtgcgttcc
    23161 gtgaaggtgg caaaggactg acaacggtcg atatgatccc acacggcgag catcgaatgg
    23221 cggtgaaact ggggctggaa aaacgttttc agttgcgcga tgacgagttt tatcccagcc
    23281 atgaggcgac ggatttttat catcgttata aagaagatat cgccctgatg gcagagatgg
    23341 gattcaaagt gttccgtacc tcaattgcct ggagccgcct cttcccgcag ggcgatgaac
    23401 tgacgcccaa ccagcagggc attgcttttt accgttctgt ctttgaagag tgtaaaaagt
    23461 atggtatcga accgctggtc acgttgtgcc acttcgatgt gccgatgcat ctggtcaccg
    23521 aatatggctc ctggcgtaac cgcaagctgg tggagctttt cagccgctac gcccggacct
    23581 gctttgaagc atttgatggt ctggtgaaat actggctaac cttcaatgaa atcaacatta
    23641 tgttgcatag cccgttctcc ggcgcgggtc tggtgtttga agaaggtgaa aatcaggatc
    23701 aggtgaaata tcaggccgcg catcaccagc tggttgccag tgcgctagcc accaaaatcg
    23761 cccatgaggt taacccgcaa aatcaggtgg gctgtatgct ggcgggcggt aacttctacc
    23821 cttacagttg caagccggaa gatgtctggg cggcgctgga gaaagaccgg gaaaacctgt
    23881 tttttatcga tgtgcaggcg cggggcgcgt atccggctta ctctgcccgc gtattccgcg
    23941 aaaaaggggt aaccatcgac aaagcaccgg gcgatgatga aatcctgaaa aacaccgtcg
    24001 attttgtctc tttcagctat tacgcctcgc gctgcgcctc ggcggagatg aacgccaaca
    24061 atagcagtgc ggcgaacgta gtgaaatcgc tgcgtaaccc gtatctgcaa gtgagcgact
    24121 ggggctgggg tattgatcca ctcggtctgc gtatcaccat gaacatgatg tacgaccgtt
    24181 atcagaagcc gctgtttctg gtggaaaacg gcctcggcgc aaaagatgaa cttgctgcca
    24241 atggcgagat taacgatgac tatcgcatca gctatttacg cgaacatatc cgcgcaatgg
    24301 gcgaagcgat tgcagatggc attccgctga tgggctacac cacatggggc tgtattgatt
    24361 tagtttccgc ctctacgggt gaaatgagca aacgctacgg ttttgtctac gtagaccgtg
    24421 acgacgcagg caacggcacg ctgacgcgca cgcgtaagaa atcgttctgg tggtataaaa
    24481 aagtgattgc cagtaatggg gaagatttag agtaagtaac cgtgccggat gcggcgtgaa
    24541 cgccttatcc ggcctacata gttcgtgcga c
//
LOCUS       DAZGSP010000074        24418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZGSP010000074 DAZGSP010000000
VERSION     DAZGSP010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.074"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            56..1033
                     /gene="ybeQ"
                     /locus_tag="ACHK74_003321"
     CDS             56..1033
                     /gene="ybeQ"
                     /locus_tag="ACHK74_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFP0222082.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(1103..1585)
                     /gene="ybeL"
                     /locus_tag="ACHK74_003322"
     CDS             complement(1103..1585)
                     /gene="ybeL"
                     /locus_tag="ACHK74_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFP0222083.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1820..4402
                     /gene="leuS"
                     /locus_tag="ACHK74_003323"
     CDS             1820..4402
                     /gene="leuS"
                     /locus_tag="ACHK74_003323"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFP0222084.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4417..4998
                     /gene="lptE"
                     /locus_tag="ACHK74_003324"
     CDS             4417..4998
                     /gene="lptE"
                     /locus_tag="ACHK74_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFP0222085.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4998..6029
                     /gene="holA"
                     /locus_tag="ACHK74_003325"
     CDS             4998..6029
                     /gene="holA"
                     /locus_tag="ACHK74_003325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFP0222086.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDVVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6031..6672
                     /gene="nadD"
                     /locus_tag="ACHK74_003326"
     CDS             6031..6672
                     /gene="nadD"
                     /locus_tag="ACHK74_003326"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFP0222087.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6696..7307
                     /gene="cobC"
                     /locus_tag="ACHK74_003327"
     CDS             6696..7307
                     /gene="cobC"
                     /locus_tag="ACHK74_003327"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFP0222088.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARRVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7567..7884
                     /gene="rsfS"
                     /locus_tag="ACHK74_003328"
     CDS             7567..7884
                     /gene="rsfS"
                     /locus_tag="ACHK74_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFP0222089.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7888..8355
                     /gene="rlmH"
                     /locus_tag="ACHK74_003329"
     CDS             7888..8355
                     /gene="rlmH"
                     /locus_tag="ACHK74_003329"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFP0222090.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8386..10287
                     /gene="mrdA"
                     /locus_tag="ACHK74_003330"
     CDS             8386..10287
                     /gene="mrdA"
                     /locus_tag="ACHK74_003330"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFP0222091.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10290..11402
                     /gene="mrdB"
                     /locus_tag="ACHK74_003331"
                     /gene_synonym="rodA"
     CDS             10290..11402
                     /gene="mrdB"
                     /locus_tag="ACHK74_003331"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFP0222092.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11413..12501
                     /gene="rlpA"
                     /locus_tag="ACHK74_003332"
     CDS             11413..12501
                     /gene="rlpA"
                     /locus_tag="ACHK74_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFP0222093.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12641..13852
                     /gene="dacA"
                     /locus_tag="ACHK74_003333"
     CDS             12641..13852
                     /gene="dacA"
                     /locus_tag="ACHK74_003333"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFP0222094.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13963..14226
                     /gene="ybeD"
                     /locus_tag="ACHK74_003334"
     CDS             13963..14226
                     /gene="ybeD"
                     /locus_tag="ACHK74_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFP0222095.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14327..14968
                     /gene="lipB"
                     /locus_tag="ACHK74_003335"
     CDS             14327..14968
                     /gene="lipB"
                     /locus_tag="ACHK74_003335"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFP0222096.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15227..16180
                     /gene="ybeF"
                     /locus_tag="ACHK74_003336"
     CDS             15227..16180
                     /gene="ybeF"
                     /locus_tag="ACHK74_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFP0222097.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            16389..17354
                     /gene="lipA"
                     /locus_tag="ACHK74_003337"
     CDS             16389..17354
                     /gene="lipA"
                     /locus_tag="ACHK74_003337"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFP0222098.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(17455..17658)
                     /gene="tatE"
                     /locus_tag="ACHK74_003338"
     CDS             complement(17455..17658)
                     /gene="tatE"
                     /locus_tag="ACHK74_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFP0222099.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(17787..18575)
                     /locus_tag="ACHK74_003339"
     CDS             complement(17787..18575)
                     /locus_tag="ACHK74_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFP0222100.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            18668..19051
                     /gene="crcB"
                     /locus_tag="ACHK74_003340"
     CDS             18668..19051
                     /gene="crcB"
                     /locus_tag="ACHK74_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFP0222101.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(19106..19315)
                     /gene="cspE"
                     /locus_tag="ACHK74_003341"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(19106..19315)
                     /gene="cspE"
                     /locus_tag="ACHK74_003341"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP0222102.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(19490..20050)
                     /gene="pagP"
                     /locus_tag="ACHK74_003342"
     CDS             complement(19490..20050)
                     /gene="pagP"
                     /locus_tag="ACHK74_003342"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFP0222103.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            20639..22024
                     /gene="dcuC"
                     /locus_tag="ACHK74_003343"
     CDS             20639..22024
                     /gene="dcuC"
                     /locus_tag="ACHK74_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFP0222104.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(22065..22745)
                     /gene="dpiA"
                     /locus_tag="ACHK74_003344"
                     /gene_synonym="citB"
     CDS             complement(22065..22745)
                     /gene="dpiA"
                     /locus_tag="ACHK74_003344"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFP0222105.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(22714..24372)
                     /gene="dpiB"
                     /locus_tag="ACHK74_003345"
     CDS             complement(22714..24372)
                     /gene="dpiB"
                     /locus_tag="ACHK74_003345"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFP0222106.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT         6120 a         6171 c         6041 g         6086 t
ORIGIN      
        1 aaatggaaca cataaaaata taatatgttt tatctgcaac cataaggaaa tgataatgat
       61 tttcacgtca agttgctgcg ataatttatc aatagatgag attatcgaac gtgctgaaaa
      121 aggcgattgc gaggctcaat atattgttgg gttttattat aatcgcgata gcgcaattga
      181 ttctccggac gacgaaaaag ccttttactg gctgaagctg gccgctgagc aaggtcattg
      241 tgaagcacag tattccttag ggcaaaagta taccgaggat aaaagccgtc ataaagataa
      301 tgagcaagcc atcttctggc tgaaaaaagc tgccctacaa ggccatactt tcgcttccaa
      361 cgcccttggc tggacactgg atcgtggaga agcccccaat tataaagaag cggttgtctg
      421 gtatcagata gccgcggaga gcggaatgtc ttatgcgcaa aataatcttg ggtggatgta
      481 cagaaatggc aacggagtcg caaaagacta tgcgctggca tttttctggt acaaacaagc
      541 tgcattacaa ggccatagtg acgcgcaaaa caatctggcc gatctttatg aagacggaaa
      601 aggcgttgct caaaacaaga cactcgccgc attctggtat ttgaaaagcg cacagcaggg
      661 taatcggcac gcccagtttc aaattgcatg ggattataac gctggcgaag gggtggacca
      721 ggactataag caagcgatgt actggtatct gaaggctgcc gctcaggaaa gcgtcggcgc
      781 ttacgtcaac atcggttata tgtataaaca cggacaaggc gttgagaaag attatcaggc
      841 tgcctttgaa tggtttacga aagccgctga atgcaatgac gccactgcct ggtataacct
      901 ggccattatg tatcactacg gagaaggaag acctgtcgat ctccgacagg ctctcgacct
      961 gtatcgtaaa gttcagtcat ccggaaccag agatgtcagt caggaaattc gtgagactga
     1021 agatttactg tagagataaa aacaagggcc ggggaatttc attcggccca tattctcttt
     1081 attgctgtgc gaaatcagac tattacggct caaacgggcg tctctggaac tggtcatgac
     1141 cacatttcgg gcatagcgtc agcacttccg gtgtgtagat cgggagatgg aagtgacatt
     1201 tctcgcagac cagatttccc agcccgacca cttctccgct gtgataaacc ccatgatgat
     1261 tgaggtcctg gaaaacttcg cgccattcaa gctgcgtttt atcggtgata tctgccagct
     1321 cctgccataa gctttcttta atcacccgca taaagacgct gtcagattct tctttcaggc
     1381 tctcttcata gctcatggcg aactcttcca ggtcacgtct tacagctcgc gtcagctcat
     1441 cgacctcggt tcgcgttaac tcccctgttt ttattacgcg ctcgcgcgcc tgttccacca
     1501 gtgcgtcgat atcacgttcg ccattgcgca ggcgttcgct cagtgacgcg accagttcac
     1561 ggtaatattg agcaaccttg ttcatcgttt catctcccgg gtcgttaact gactataata
     1621 atagacgttc tttgttcctt gctttgctaa tacgcccaca aactctgtaa attaatgtat
     1681 gaataatccg ggcatcattt caacgtcgag tgcgcgaaag gctgttttga cgcggacgtt
     1741 tgggctatgc tatgcggatc tgaaaaacca cctcaacgct acatttgtag ccgtattgaa
     1801 aacaggacca ctggctgcca tgcaagagca ataccgcccg gaagagatag aatccaaagt
     1861 acagcttcat tgggatgaga agcgcacatt tgaagtaacc gaagacgaga gcaaagagaa
     1921 gtattactgc ctgtctatgc ttccctatcc ttctggtcga ctacacatgg gccacgtacg
     1981 taactacacc atcggtgacg tgatcgcccg ctaccagcgt atgctgggca aaaacgtcct
     2041 gcagccgatc ggctgggacg cgtttggtct gcctgcggaa ggcgcggcgg tgaaaaacaa
     2101 caccgctccg gcaccgtgga cgtacgacaa catcgcgtat atgaaaaacc agctcaaaat
     2161 gctgggcttt ggttatgact ggagccgcga gctggcaacc tgtacgccgg aatactaccg
     2221 ttgggaacag aaattcttca ccgagctgta taaaaaaggc ctggtatata agaagacttc
     2281 tgcggtcaac tggtgcccga acgaccagac cgtactggcg aacgaacaag ttatcgacgg
     2341 ctgctgctgg cgctgcgata ccaaagttga acgtaaagag atcccgcagt ggtttatcaa
     2401 aatcactgct tacgctgacg agctgctcaa cgatctggat aaactggatc actggccaga
     2461 caccgttaaa accatgcagc gtaactggat cggtcgttcc gaaggcgtgg agatcacctt
     2521 caacgttaac gactatgaca acacgctgac cgtttacact acccgcccgg acacctttat
     2581 gggttgtacc tacctggcgg tagctgcggg tcatccgctg gcgcagaaag cggcggaaaa
     2641 taatcctgaa ctggcggcct ttattgacga atgccgtaac accaaagttg ccgaagctga
     2701 aatggcgacg atggagaaaa aaggcgtcga tactggcttt aaagctgttc acccattaac
     2761 gggcgaagaa attcccgttt gggcagcaaa cttcgtattg atggagtacg gcacgggcgc
     2821 agttatggcg gttccgggtc acgaccagcg cgactacgag tttgcctcta aatacggcct
     2881 gaacatcaaa ccagttatcc tggctgctga cggctctgag ccagatcttt ctcagcaagc
     2941 cctgactgaa aaaggcgtgc tgttcaactc tggcgaattc aacggtcttg accatgaagc
     3001 ggccttcaac gccatcgccg ataaactgac tgcgatgggc gttggcgagc gtaaagtgaa
     3061 ctaccgcctg cgcgactggg gtgtttctcg tcagcgttac tggggcgcgc cgattccgat
     3121 ggtgacgctg gaagacggta ccgtaatgcc gaccccggac gaccagctgc cggtgatcct
     3181 gccggaagat gtggtgatgg acggcattac cagcccgatt aaagcagatc cggagtgggc
     3241 aaaaactacc gttaacggta tgccagcgct gcgtgaaacc gacactttcg acacctttat
     3301 ggagtcctcc tggtactatg cgcgctacac ttgcccagag tacaaagaag gtatgctgga
     3361 ttccgaagcg gctaactact ggctgccggt ggatatctac attggtggta tcgaacacgc
     3421 cattatgcac ctgctctact tccgcttctt ccacaaactg atgcgtgatg caggcatggt
     3481 gaactctgac gaaccagcga aacagttgct gtgtcagggt atggtgctgg cagatgcctt
     3541 ctactatgtt ggcgaaaacg gcgaacgtaa ctgggtttcc ccggttgatg ctatcgttga
     3601 acgtgacgag aaaggccgta tcgtgaaagc gaaagatgcg gcaggccatg aactggttta
     3661 taccggcatg agcaaaatgt ccaagtcgaa gaacaacggt atcgacccgc aggtgatggt
     3721 tgaacgttac ggcgcggaca ccgttcgtct gtttatgatg tttgcttctc cggctgatat
     3781 gactctcgaa tggcaggaat ccggtgtgga aggggctaac cgcttcctga aacgtgtctg
     3841 gaaactggtt tacgagcaca cagcaaaagg tgatgttgcg gcactgaacg ttgatgcgct
     3901 gactgaagat cagaaagcgc tgcgtcgcga tgtgcataaa acgatcgcta aagtgaccga
     3961 tgatatcggc cgtcgtcaga ccttcaacac cgcaattgcg gcgattatgg agctgatgaa
     4021 caaactggcg aaagcaccaa ccgatggcga gcaggatcgc gctctgatgc aggaagcgct
     4081 gctggccgtt gtccgtatgc ttaacccgtt caccccgcac atctgcttca cgctgtggca
     4141 ggaactgaaa ggcgaaggcg atatcgacaa cgcgccgtgg ccggttgctg acgaaaaagc
     4201 gatggtggaa gactccacgc tggtcgtggt gcaggttaac ggtaaagtcc gtgccaaaat
     4261 caccgttccg gtggacgcaa cggaagaaca ggttcgcgaa cgtgctggcc aggaacatct
     4321 ggtagcaaaa tatcttgatg gcgttaccgt gcgtaaagtg atttacgtac caggtaaact
     4381 cctcaatctg gtcgttggct aagcgcggga ggaagcgtgc gatatctggc aacattgttg
     4441 ttatctctgg cggtgttaat caccgccggg tgtggctggc atctgcgtga taccacgcag
     4501 gttccttcca ctatgaaggt catgatcctg gactcaggcg atccgaacgg gccattaagc
     4561 cgtgcggtgc gtaaccagtt acgtctgaat ggtgtcgagt tgcttgataa agaaaccacg
     4621 cgtaaggacg ttccatccct gcgtttgggt aaagtgagca tcgcgaaaga taccgcatcg
     4681 gtattccgta acggtcaaac agcagagtat cagatgatca tgacggtgaa tgcgaccgtg
     4741 ttgatccccg gccgtgatat ctacccgatt agtgccaaag tcttccgttc gttctttgat
     4801 aacccgcaaa tggcgttagc gaaagataac gaacaagaca tgatcgtaaa agagatgtac
     4861 gaccgtgctg ccgaacagct gattcgtaag ctgccaagca tccgtgctgc ggatattcgt
     4921 tccgacgaag aacagacgtc gaccacaacg gatactccgg caacgcctgc acgcgtctcc
     4981 accacgctgg gtaactgatg attcggttgt acccggaaca actccgcgcg cagctcaatg
     5041 aagggctgcg cgcggcgtat cttttacttg gtaacgatcc tctgttattg caggaaagcc
     5101 aggacgttgt tcgtcaggta gctgcggcac aaggattcga agaacaccac actttttcca
     5161 ttgatcccaa cactgactgg aatgcgatct tttcgttatg ccaggctatg agtctgtttg
     5221 ccagtcgaca aacgctattg ctgttgttac cagaaaacgg accgaatgcg gcgatcaatg
     5281 aacaacttct cacactcacc ggacttctgc atgacgacct gctgttgatc gtccgcggta
     5341 ataaattaag caaagcgcaa gaaaatgccg cctggtttac tgctcttgcg aatcgcagcg
     5401 tgcaggtgac ctgtcagaca ccggagcagg ctcagcttcc ccgctgggtt gctgcgcgcg
     5461 caaaacagct caacttagaa ctggatgacg ctgcaaatca ggtgctctgc tactgttatg
     5521 aaggtaacct gctggcgctg gctcaggcac tggagcgttt atcgctgctc tggccagacg
     5581 gcaaattgac attaccgcgc gttgaacagg cggtgaatga tgccgcgcat ttcacccctt
     5641 ttcattgggt tgatgctttg ttgatgggaa aaagtaagcg cgcgttgcat attcttcagc
     5701 aactgcgtct ggaaggcagc gaaccggtta ttttgttgcg cacattacaa cgtgaactgt
     5761 tgttactggt taacctgaaa cgccagtctg cccatacgcc actgcgtgcg ttgtttgata
     5821 agcatcgggt atggcagaac cgccggggca tgatgggcga ggcgttaaat cgcttaagtc
     5881 agacgcagtt acgtcaggcc gtgcaactcc tgacacgaac ggaactcacc ctcaaacaag
     5941 attacggtca gtcagtgtgg gcagagctgg aagggttatc tcttctgttg tgccataaac
     6001 ccctggcgga cgtatttatc gacggttgat atgaaatctt tacaggctct gtttggcggc
     6061 acctttgatc cggtgcacta tggtcatctt aaacccgtgg aaacgctggc gaatttgatt
     6121 ggtctgacgc gggtcacaat catccctaat aatgttcctc cgcatcgtcc ccagccggaa
     6181 gcgaacagcg tgcagcgtaa acacatgctt gaactggcga ttgccgacaa gccattattt
     6241 actcttgatg aacgcgagct aaagcgcaat gccccctctt acactgcgca aacactgaaa
     6301 gagtggcggc aggaacaagg gccggacgtg ccgctggcgt ttattattgg tcaggattca
     6361 ttgctgacct ttccgacctg gtacgaatac gaaacgatac tcgacaatgc acatttgatc
     6421 gtctgtcggc gtccaggtta cccacttgaa atggcgcaac cgcaatacca gcaatggctg
     6481 gaagatcatt tgacacataa cccggaagat cttcaccttc agcctgccgg taaaatttat
     6541 ctggctgaaa cgccgtggtt taacatctcg gcgaccatca tccgcgaacg tttgcaaaac
     6601 ggtgaatcgt gtgaggattt attgccggaa ccggtactga cttacattaa ccaacaaggc
     6661 ttgtatcgct gatacctgtc gttacggagg gcatgatgcg actgtggtta attcgtcatg
     6721 gtgaaacgca agcgaatatc gatggtcttt acagcggtca tgcgcccacc cccctgaccg
     6781 cgcgcggtat tgagcaagcg caaaatctgc atacactgct agatgatgtt tcctttgacc
     6841 tggttttatg cagtgaactg gaacgggcac agcataccgc gcgacgggtt ctcagtgacc
     6901 gccagctccc cgtgcaaatc atccctgaac tcaacgaaat gttttttggc gactgggaga
     6961 tgcgacatca tcgcgacctc atgcaagaag atgccgaaaa ctatagcgcg tggtgcaatg
     7021 actggcagca tgctatcccc acgaacggtg aaggatttca ggcattttcg caacgtgtgg
     7081 aaagatttat cgcaaggctt agtgaatatc agcactatca gaatatttta atcgtcagcc
     7141 atcagggcgt actgagtctg ttaattgctc gtctaattgg catgcctgcc gaatctatgt
     7201 ggcattttcg cgttgaccag gggtgttgga gcgccattga tatcaaccaa aaattcgcga
     7261 cgctacgcgt cctcaatagc cgtgccatcg gggtcgagaa tgcatgactt ttctgttttt
     7321 ttacgggtaa gccgcaacga ccattgacag ccccgggcag gctgatattc tccgcagcca
     7381 gactttttcc gccagacacg actttgtaga aattgtttta caaaaatggc gatgcaatct
     7441 gcggcgcggg gtaggatgat agcccacttt cgaaagccga ttcggcgaca attgtcccga
     7501 aatcgcctct ggttcaggta tactgacaga ccatttttat ctatttgatt cacccagggg
     7561 gaaaacttgc agggtaaagc actccaggat tttgttatcg acaaaattga tgacctcaaa
     7621 ggtcaggaca tcatcgcctt agacgttcag ggcaaatcca gcatcaccga ctgcatgatc
     7681 atctgtacgg gtacgtccag ccgtcatgtt atgtccattg ctgaccacgt tgtgcaggag
     7741 tctcgcgcag cgggcctgtt accgctcggc gtagaaggtg aaaacagcgc cgactggatt
     7801 gtcgtggatt tgggcgatgt gattgtccat gtcatgcagg aagagagccg tcgcctgtat
     7861 gaactggaaa aactctggag ttaatgcgtg aagctgcaac ttgtcgccgt gggaacgaaa
     7921 atgccggact gggtacaaac cggttttacc gagtacctgc gtcgttttcc gaaagatatg
     7981 cccttcgagc tgattgaaat tccggccgga aaacgcggca agaatgcgga catcaagcgc
     8041 atactcgaca aagagggtga gcagatgttg gcggccgcag gcaaaaaccg cattgtcacc
     8101 ctcgatattc caggcaagcc ctgggatacg ccgcagttag ccgctgagct ggaacgctgg
     8161 aagctggatg gtcgcgacgt cagtctactg attggcgggc ctgaagggtt gtcgcctgcc
     8221 tgtaaagcgg cggcggagca gagctggtcg ctgtcggcgc ttaccctccc ccatccgctg
     8281 gttcgcgtgc tggtcgcaga gagtctgtac cgggcgtgga gcatcaccac caaccatcct
     8341 tatcaccgtg agtgataagg gagctttgag tagaaaacgc agcggatgaa actacagaac
     8401 tcttttcgcg actatacggc agagtccgcg ctgtttgtgc gccgggcgct ggtcgccttt
     8461 ttggggattt tgctgctgac cggcgtgctt atcgccaacc tgtataatct gcaaattgtt
     8521 cgctttaccg attaccagac ccgctctaat gaaaaccgca ttaagctggt gcctatcgcg
     8581 cccagccgcg gcattatcta cgaccgtaac ggtatccctc tggcactcaa ccgcactatc
     8641 taccagatag aaatgatgcc ggagaaagtc gataacgtgc agcaaacgct ggacgctttg
     8701 cgcagcgtgg ttgatctgac cgatgacgat attgctgcat tccgaaaaga gcgcgcacgt
     8761 tcacaccgtt tcacctctat tcccgtgaaa actaacctga ccgaagtaca agtagctcgc
     8821 tttgccgtca atcagtaccg ttttccgggt gtcgaagtta aaggctataa acgtcgttac
     8881 tatccttacg gttcggcgtt gacccacgtc atcggctatg tgtcgaaaat caacgataaa
     8941 gacgtcgaac gcctgaataa tgacggcaaa ctggccaact atgcggcaac gcatgatatc
     9001 ggtaagctgg gcattgagcg ttactatgaa gacgtgctgc acggtcagac cggttatgaa
     9061 gaggttgaag ttaacaaccg tgggcgtgtt atccgccagt taaaagaagt accaccgcaa
     9121 gccggacacg atatttacct gacgctggat ctcaaactcc agcaatatat tgaaacgctg
     9181 ctggcgggta gccgcgcagc tgtggtagtc accgatccgc gtacaggtgg ggtgctggca
     9241 ctggtttcca cgcctagtta tgacccaaac ctgtttgttg acggtatctc cagcaaagat
     9301 tattccgcct tgctgaacga cccgaataca ccgctggtga accgcgccac acagggggtt
     9361 tatcctcctg cgtctacagt taaaccctat gtggcggttt cggcattaag cgccggggtg
     9421 atcacgcgca atacgacgct gtttgaccca ggctggtggc aactaccagg gtcggaaaaa
     9481 cgttatcgtg actggaaaaa atggggccac gggcgtctga atgtcacaag atcgctggaa
     9541 gaatctgcgg ataccttctt ctatcaggtg gcctacgata tggggatcga tcgcctctcc
     9601 gaatggatgg gtaaattcgg ttatggtcat tacaccggta tcgacctggc ggaagaacgt
     9661 tccggcaaca tgcctacccg cgaatggaaa cagaaacgct ttaaaaaacc gtggtatcag
     9721 ggtgacacca ttccggttgg tatcggccag ggttactgga cagcgacccc aatccagatg
     9781 agtaaggcac tgatgatcct gattaatgac ggtatcgtga aggttccgca tttgctgatg
     9841 agcaccgccg aagacggcaa acaggtgcca tgggtacaac cgcatgaacc gcccgtcggc
     9901 gatattcatt ccggttactg ggagctggcg aaagacggta tgtacggtgt tgctaaccgc
     9961 cctaacggta cggcgcataa atactttgcc agcgcaccgt acaaaattgc ggcgaaatct
    10021 ggtaccgctc aggtcttcgg tctgaaagcg aacgaaacct ataatgcgca caaaattgcc
    10081 gagcgtttac gtgaccacaa actgatgacc gcctttgcgc catacaacaa tccgcaagtg
    10141 gctgtcgcca tgattctgga gaacggcggc gcgggtccgg cggttggtac gctgatgcgc
    10201 cagatcctcg accacattat gctgggtgat aacaataccg atctgcctgc ggaaaatcct
    10261 gcggttgccg cagcggagga ccattaatca tgacggataa tccgaataaa aaaacattct
    10321 gggataaagt ccatctcgat cccacaatgc tgctgatctt actggcattg ctggtttaca
    10381 gcgccctggt tatctggagc gccagcggtc aggatattgg catgatggag cgtaaaatag
    10441 gccaaatcgc gatgggtctg gtcatcatgg tggtgatggc gcaaattcct ccacgcgttt
    10501 atgaaggctg ggccccctat ctctatatca tctgtattat tttgctggtg gcggtagatg
    10561 ctttcggtgc catctctaaa ggcgctcaac gctggctgga cctcggtatt gttcgttttc
    10621 agccgtcgga aattgccaaa atagccgtac cactgatggt tgcgcgcttt atcaaccgcg
    10681 acgtttgccc gccatcgttg aagaacactg gcatcgcgct ggtgctgata tttatgccca
    10741 cgctgctggt ggctgcacag cctgacctgg gaacatcaat cctcgttgcg ctttccggtc
    10801 tgtttgtact gttcctctct ggccttagct ggcgtctgat tggcgtcgca gtagtgctgg
    10861 tggcggcgtt cattcctatt ctgtggttct tcctgatgca tgattaccag cgccagcgcg
    10921 taatgatgct cctggacccg gaatcagacc cactcggcgc gggctatcac attattcagt
    10981 ctaaaattgc tattggctcc ggcggattac gcggcaaagg ctggctgcat ggcactcagt
    11041 cacaacttga atttctaccc gaacgccata ccgactttat cttcgcggta ctggcggaag
    11101 agctgggatt agtgggcatt ctgattctgc tcgctctcta cattctgctg atcatgcgcg
    11161 ggctgtggat agccgccaga gcgcaaacca cctttggtcg cgtcatggct ggcggcttaa
    11221 tgctgatatt attcgtttat gtcttcgtaa atattggtat ggtaagcggt attctgccgg
    11281 ttgtaggggt tccgctccca ctggtcagtt atggaggatc agcgctaatt gtgctgatgg
    11341 ctgggttcgg gattgtaatg tcaatccaca cccacaggaa aatgttgtcg aaaagcgtgt
    11401 aagaggtgcg caatgcgtaa gcagtggctc gggatctgca tcgcggcagg aatgctcgcg
    11461 gcatgtacaa gcgatgatgg tcagcaacag acggtaagtg taccgcagcc tgcggtatgt
    11521 aacggcccca tagttgaaat tagcggggcg gacccgcgtt tcgaaccact gaacgcgacg
    11581 gcaaatcagg attaccagcg cgacggtaaa agctacaaaa tcgtgcagga tccgtctcga
    11641 tttagccagg cgggactggc ggcaatctat gatgccgaac caggcagtaa cctgacggcc
    11701 tctggcgaag ctttcgatcc gacacagttg acggcggccc atccaacgct tccgatcccc
    11761 agctacgcca gaatcactaa cctggctaac gggcgaatga tcgtggtgcg cattaatgat
    11821 cgcggcccct acggcaacga ccgcgtaatt tcgctttctc gcgcagcagc tgaccgtctt
    11881 aacacgtcaa acaacaccaa agttcgtatc gatccgatta ttgtcgccca ggatggttcg
    11941 ctttctggtc ctggtatggc ttgtaccaca gtcgccaaac agacttacgc cctgcctgca
    12001 cctcccgatt taagcggtgg cgcgggggca agttcagtgt ctggcccgca gggtgacatt
    12061 cttccggtca gtaactcgac gctaaaaagc gaagatccga ccggcgcgcc ggtaaccagt
    12121 agcggtttcc tcggcgctcc aacgacctta gcgcctggtg tactggaaag cagcgaaccg
    12181 acacctgcac cacagcccgt tgttacagct ccgtcgacaa cgcctgcaac ctcgcctgca
    12241 atggtgacac cgcaagccgc ctcgcaaagc gccagcggca actttatggt gcaagtcggg
    12301 gccgttagcg atcaggctcg tgcgcaacag taccaacagc aactgggaca gaagttcggc
    12361 gtccccggtc gcgtaactca aaatggcgcg gtctggcgta tccagcttgg cccattcgcc
    12421 agtaaagccg aagccagtac cttgcagcaa cgtttgcaaa ccgaagccca attacagtca
    12481 tttattacca ccgcgcagta gcgtaaagca ggcatctgat gtgtcaattt ctgtaaatga
    12541 cgttaacaaa gtcacgcgga aagtcagatg cctgccggta gtgcatttgc tatagtaggg
    12601 cacttttttt aattccatca cggatgtcgt agttcagacc atgaatacca ttttttccgc
    12661 tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc acagccttta tctctgctgc
    12721 acatgccgat gacctgaata tcaaaactat gatcccgggt gtaccgcaga tcgatgcgga
    12781 gtcctacatc ctgattgact ataactccgg caaagtgctc gccgaacaga acgcagatgt
    12841 ccgccgcgat cctgccagcc tgaccaaaat gatgaccagt tacgttatcg gccaggcaat
    12901 gaaagccggt aaatttaaag aaactgattt agtcactatc ggcaacgacg catgggccac
    12961 cggtaacccg gtgtttaaag gttcttcgct gatgttcctc aaaccgggca tgcaggttcc
    13021 ggtttctcag ctgatccgcg gtattaacct gcaatcgggt aacgatgctt gcgtcgccat
    13081 ggccgatttt gccgctggta gccaggacgc ttttgttggc ttgatgaaca gctacgttaa
    13141 cgccctgggc ctgaaaaaca cccacttcca gacggtacat ggtctggatg ctgatggtca
    13201 gtacagctcc gcgcgagata tggcgctgat cggccaggca ttgatccgtg acgtaccgaa
    13261 tgaatactcg atctataaag aaaaagaatt tacgtttaac ggtattcgcc agctgaaccg
    13321 taacggcctg ttatgggata acagcctgaa tgtcgacggc atcaaaaccg gacacactga
    13381 caaagcaggt tacaaccttg ttgcttctgc gactgaaggc cagatgcgct tgatttctgc
    13441 ggtgatgggc ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac tgctaacctg
    13501 gggcttccgt ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt tcgcctctga
    13561 accggtttgg tttggtgatt ctgatcgcgc ttcgttaggg gttgataaag acgtgtacct
    13621 gaccattcca cgtggtcgca tgaaagatct gaaagccagc tatgtgctga acagcagtga
    13681 attgcatgcg ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc agcttgatgg
    13741 caaaacgatc gagcaacgcc cgctggttgt gttgcaagaa atcccggaag gtaacttctt
    13801 cggcaaaatc attgattaca ttaaattaat gttccatcac tggtttggtt aaaaattgaa
    13861 cacttgaaag tgtaatttcc gtccccatat actaagcatc agtaaaaaaa ctcccgcctt
    13921 ctggcgggag ttgctattta attacgttac gccggagctg acatgaaaac caaacttaac
    13981 gaactgcttg aattccctac tccttttact tacaaagtta tggggcaggc gttacctgag
    14041 ctggttgatc aggtggttga agtggtacag cgccatgcgc caggtgacta caccccaacg
    14101 gtaaaaccaa gcagcaaagg caactaccac tcggtatcta tcactatcaa cgccactcat
    14161 atcgagcagg ttgaaacact gtatgaagaa ctgggcaaaa tcgatattgt ccgcatggtt
    14221 ctgtaactcg cttctccgtt acccggctcc tggtcgggta actcccctct cctcgctgtg
    14281 atatactttt cccccacttt tactcattct ccacggagat gccgttttgt atcaggataa
    14341 aattcttgtc cgccagctcg gtcttcagcc ttacgagcca atctcccagg ctatgcatga
    14401 attcaccgat acccgcgatg atagtaccct tgatgaaatc tggctggtcg agcactatcc
    14461 ggtattcacc caaggtcagg caggaaaagc ggagcacatt ttaatgccgg gtgatattcc
    14521 ggtgatccag agcgatcgcg gtgggcaggt gacttatcac gggccgggac aacaggtgat
    14581 gtatgtgttg cttaacctga aacgccgtaa actcggtgtg cgtgaactgg tgaccttgct
    14641 tgagcaaaca gtggtgaata ccctggctga actgggtata gaagcgcatc ctcgggctga
    14701 cgcgcctggt gtctatgtcg gggaaaagaa aatttgctca ctgggtttac gaattcgacg
    14761 cggttgttca ttccacggtc tggcattaaa cgtcaatatg gatctttcac catttttacg
    14821 tattaatcct tgtgggtatg ccggaatgga aatggctaaa atatcacaat ggaaacccga
    14881 agcgacgact aataatattg ctccacgttt actggaaaat attttagcgc tactaaacaa
    14941 tccggacttc gaatatatta ccgcttaatt ccatacatca atggcccaat ttacactggg
    15001 tcattactca taatacctgc taatgcttta catctccttc gcgtttgtat attttcttta
    15061 tcacttgcag aaatgcggtg ccacgccttg ctccgcaact caccccattg attaacctat
    15121 tgaaatagtt gattatggtg agcaaaagcg ttccgttcca attagacata agattattgt
    15181 aattttttaa atattatttt tccatgaata aaattggagt aagtgcgtgg atagtaataa
    15241 tcaaattgaa ccctgtttaa gcagaaagtc gtcagaaggt aaaccacaaa tatttacaac
    15301 cttacgaaat attgatctta accttctgac tatttttgaa gctgtatatg tacataaagg
    15361 gatcgttaat gcagcgaaag tgcttaatct gaccccctcg gcaatcagtc agtctattca
    15421 gaaactgcgc gttatattcc ctgacccatt gtttattcgc aaaggccagg gtgtcactcc
    15481 taccgcattt gcgatgcatc tacatgagta tatcagtcag ggccttgagt ccattcttgg
    15541 tgcgctggat atcgaaggaa gctatgataa gcaacgaacg ataactattg cgaccactcc
    15601 ctcggtcgga gccttggtcc ttcctgtcat ctatcgggcg attaaaactc actatccgca
    15661 gcttttactg cgcaacccgc ccatcagcga cgcggaaaac caactcagtc agtttcaaac
    15721 cgatctcatc atcgataaca tgttttgcac caatcgtacg gtgcaacatc atgttctgtt
    15781 caccgacaat atggtgttaa tttgccgcga gggaaatcca ctactctctt tagaagatga
    15841 cagagagact atcgacaacg ctgcgcatgt acttctgtta ccggaagagc aaaatttcag
    15901 cggtctgcgg cagagagttc aagaaatgtt tccggaccgg caaatcaatt tcaccagcta
    15961 caacattttg acaatcgctg cactggttgc caacagtgac atgttagcga ttattcccag
    16021 ccgtttttat aacctgttta gccgctgctg gccgctggaa aaactgcctt ttccgtcctt
    16081 aaatgaggag caaatagatt tctctatcca ctacaacaaa ttcagtctgc gtgatcccat
    16141 cctgcacgga gtaatagatg ttatccgtaa tgcattttga aaaagtattg ttacagccag
    16201 atcacagcgc agaacgataa aatggcacaa tccaacaaca attttacatt ttcgcgaccg
    16261 ctttggctgc tttcaggtcc gtttcaatga tatactgcca gtcgttaatt caaaaatagt
    16321 tgataattac aacaatctat tgaattgaaa cgctttcctt cgtaattcgc aactggaaca
    16381 cgcacgctat gagtaaaccc attgtgatgg aacgcggtgt taaataccgc gatgccgata
    16441 agatggccct tatcccggtt aaaaacgtgg caacagagcg cgaagccctg ctgcgcaagc
    16501 cggaatggat gaaaatcaag cttccagcgg actctacacg tatccagggc atcaaagccg
    16561 caatgcgcaa aaatggcctg cattctgtct gcgaggaagc ctcctgccct aacctggcgg
    16621 aatgcttcaa ccacggcaca gcaacgttta tgatcctcgg cgctatttgt acccgccgtt
    16681 gtccgttctg tgatgttgcc cacggtcgcc cggtagctcc tgatgccaat gaaccagtga
    16741 aactggcgca gaccattgcc gatatggcgc tgcgttatgt ggttatcacc tccgttgacc
    16801 gtgatgacct gcgcgatggc ggtgcccagc actttgcgga ttgcattact gccattcggg
    16861 aaaaaagccc gcaaatcaaa attgaaactc tggtgccgga tttccgtggt cgtatggatc
    16921 gtgctctgga tattctgact gcaacgccac cagatgtgtt caaccataac ctggaaaacg
    16981 taccgcgtat ttaccgtcag gtacggcctg gtgcagatta caactggtcg ctgaagctgc
    17041 tggaacgctt taaagaagcg catccggaaa tcccgaccaa gtctggtctg atggtgggac
    17101 tgggtgaaac caatgaagaa attattgagg taatgcgcga cctgcgccgt catggtgtga
    17161 cgatgttaac gctggggcaa tatttgcagc caagccgcca tcacctgccg gttcaacgtt
    17221 acgttagccc ggatgagttc gacgaaatga aagccgaagc gctggcgatg ggctttaccc
    17281 atgctgcatg cggtccgttt gtccgctctt cttaccacgc cgatttgcag gcgaaaggga
    17341 tggaagttaa gtaatcctgt aataattact tacatctgtc tgataaaaaa cccgcttttt
    17401 gaagcgggtt ttttgtatcg aacaagatat tgagggagcg tcctgctcgc cacgtcactc
    17461 tttatgagag agcttttcag cctgaagatc aacgtctgcg ccttttttcg ccgcagcatc
    17521 gtcatcattc atcgccttct tgaacccttt aatggccgct ccaaggtctc cgcccagcgt
    17581 acgtaacttc ttagtcccaa acagcagaac gaccagcgcc gcaactacta gcagtttggt
    17641 aatactaatc tcacccatag ataccttctt gacatataac cggcaacaga acgcgcctta
    17701 aaaaccagag aatattaacg gtcattcgac gcgcgcacac aatagcaatc tgtaacaagg
    17761 tgaatcaagc gccgagtaaa aaaacatcat aataattgcg gcggcgcaaa gcgacggttg
    17821 tttaagacgg gcagttgcgc gcgcacctga cgcacacgtt cgggcgtcac ttccgccata
    17881 atgagtgcag gcatttctga cgctgccgca atggtgacgc caaagggatc tataatccgg
    17941 ctttgaccga tatttttgtt cccgcactcc cccgccgcca ccatataaca ggtggtatcc
    18001 agcgcacgag cggcaagcaa cgttgaccaa tgatgctctt tgagcggccc gcgaacccag
    18061 gcggcaggaa gtaccaggat ttcagctccc tgtaatgcct gtgccagcgc cagctctgga
    18121 aagcgtaagt cataacaggt catcagaccg accttcatcc cctccacctc gagtaacgga
    18181 gcgatttcat taccagcatc aacacggcgt gattcctgaa tggcaaatgc atcatagaga
    18241 tgcaatttgg cataacgggc gacgatgttt cctgcctgta gcgccaccag catattccat
    18301 gcgcgccccg gcgttgaagg aacatgaatc gtcagaattg tcgtcatcat gttacgttta
    18361 ctttctcgcc gtaaacgtcc gaggaattcg ccttccagca gctgtgctga tttaaccgat
    18421 agatctgcat catgatcatc gcgcgccagc aatgcttccg gcaggacgaa cagcgatacg
    18481 tcgttttccg ccgcctgcgc catcaacgag gcacaaatct cagcgttctt ttcccacaca
    18541 gatgtaacag caaactgtcc tgcggcaacc agcattgcca cctcctgtca gagtcgtaaa
    18601 attcgccaac gacgattatt cggcgttaca cttatcactc atacaaatca aatagcagga
    18661 ttttgcagtg ttacaacttc ttttagcagt ttttattggc ggtggtacgg gaagcgtggc
    18721 gagatggctg ttaagtatgc gatttaaccc gctgcatcag gcgattccgt tggggacgct
    18781 ggcagcaaac ctgattgggg cattcatcat aggaatggga ttcgcctggt tcagcaggat
    18841 gacgaacatt gatccagtgt ggaaagtatt aatcaccacc ggattttgtg gcggtctaac
    18901 aaccttctca acattttcgg cagaagtggt gtttttgtta caagagggcc gctttggctg
    18961 ggcgttactg aacgttttcg tcaaccttct ggggtctttt gccatgacag cactggcttt
    19021 ctggctgttt tcggcctcaa ccgcacacta aagaccaaaa aaaaacccgc tgattaagcg
    19081 ggttttgaat tcttgctgac gtatcttaca gagcgattac gtttgcagca gaagggcctt
    19141 tggcaccgtt agtgatttcg aactctacgc gctgaccttc agcaagagtt ttaaaaccat
    19201 tagtctggat tgcagagaag tgtacgaaca cgtctttgct gccgtcttcc ggagtaatga
    19261 aaccgaatcc tttggactca ttaaaccact taacgttacc tttaatctta gacatcaaaa
    19321 ttacctttac atgaaaaata gacacaaatg ctgtgtcggt taccagtaca ccaattgtgg
    19381 tacgctttgt ccagtgcaac tttactaaaa agtgataaaa gtcgcgttat ttttgatgtt
    19441 acttatttta gctattgatt ttaaagaagt tactaaaact tcatttgtct caaaactgaa
    19501 agcgcatcca ggcaaagtac acattgccat tgttgtaggt acccggaatg taggtcatct
    19561 gaaaagtcac tgggccataa cccacggagg ccaatggcag tagaaccggg agagggatgt
    19621 aattccagtt atcgcgtgcc gttacgccag cggtgaatcc cagacctaaa tgaaaatttt
    19681 catccgccag cggtcgccag gtactttccc atccgtatcc ggcaatcggt tcccatttgt
    19741 tccacgagtc cttaaatgcc atggcataca ggccatgcca gtttcctttt tcatcccaac
    19801 gcgacaggcc aaaaccgcca ccccacggtc gctcgttata gcgatcggtt ttttctttgt
    19861 cgtaagcgaa acgtgcatgc caggtgatgg caggaatata taaatcataa tgttcaggct
    19921 gttgccaggt ttgtgcaata ttttctctaa acgttgtcat ccactcatct gcgttagcaa
    19981 aaactttacc aacgctgatt aactgaataa aaacaaagga aaagatagcg acatatttac
    20041 tcacgttcat ttgtgaccat aaaacattta tcaaaaatct actactagca tagcaaagct
    20101 acaattaaca taaccttaat agacccaaca taaagaataa tctgaatagc tgctctattt
    20161 atttccgctt gttcaagtta aatatttccg cgcatcgatt aaagatgatg cgtcgttttc
    20221 tgcgatggga atagtcaaaa aagaaaaacc aagtcttttt tgatgacaaa atgcaatcaa
    20281 ggaaaaatta ttttattttt taactagata caacaatcat cttaacgaag tatataatat
    20341 aaatcgtcaa cctatgctca tgttagatta ttttttgagc gcacagccgc agctaacacc
    20401 attgcaatta acaaatttgc atcaatccac catcaattta cacacattat tatgtgataa
    20461 ttgccaaccg ctaaatatgc gttttgttat ctatgtataa aaacagcaac ttcaatgtct
    20521 taatggcagt ttttcttgat tttaatcagc attcatcgcc aatttattgg gcatattttt
    20581 tccttaagct ttaggaattt tttattattt actttggggc ctggagacag gaaaaattat
    20641 gctgacattc attgaactcc ttattggggt tgtggttatt gtgggtgtag ctcgctacat
    20701 cattaaaggg tattccgcca ctggtgtgtt atttgtcggt ggcctgttat tgctgattat
    20761 cagtgccatt atggggcaca aagtgttacc gtccagccag gcttcaacag gctacagcgc
    20821 cacggatatc gttgaatacg ttaaaatatt actaatgagc cgcggcggcg acctcggcat
    20881 gatgattatg atgctgtgtg gatttgccgc ttacatgacc catatcggcg cgaatgatat
    20941 ggtggtcaag ctggcgtcaa aaccattgca gtatattaac tccccttacc tgctgatgat
    21001 tgccgcctat tttgtcgcct gtctgatgtc tctggccgtc tcttccgcaa ccggtctggg
    21061 tgttttgctg atggcaaccc tatttccggt gatggtaaac gttggtatca gtcgtggcgc
    21121 agctgctgcc atttgtgcct ccccggcggc gattattctc gcaccgactt caggggatgt
    21181 ggtgctggcg gcgcaagctt ccgaaatgtc gctgattgac ttcgccttca aaacgacgct
    21241 gcctatctca attgctgcaa ttatcggcat ggcgatcgcc cacttcttct ggcaacgtta
    21301 tctggataaa aaagagcaca tctctcatga aatgttagat gtcagtgaaa tcaccaccac
    21361 tgctcctgcg ttttatgcca ttttgccgtt cacgccgatc atcggtgtac tgatttttga
    21421 cggtaaatgg ggtccgcaat tacacatcat cactattctg gtgatttgta tgctgattgc
    21481 ctccattctg gagttcctcc gcagctttaa tacccagaaa gttttctctg gtctggaagt
    21541 ggcttatcgc ggaatggcag atgcgtttgc taacgtggtg atgctgctgg ttgccgctgg
    21601 ggtattcgct caggggctta gcaccatcgg ctttattcaa agtctgattt ctatcgctac
    21661 ctcgtttggt tcggcgagta tcatcctgat gctggtattg gtgattctga ccatgctggc
    21721 ggcagtcacg accggttcag gcaatgcgcc gttttatgcg tttgttgaga tgatcccgaa
    21781 actggcgcac tcttccggca ttaacccggc gtatttgact atcccgatgc tgcaggcgtc
    21841 aaacctgggc cgtacccttt cgcccgtttc tggcgtagtc gttgcggttg ccgggatggc
    21901 gaagatctcg ccgtttgaag tcgtaaaacg cacctcggta ccggtgcttg ttggtttggt
    21961 gattgttatc gttgctacag agctgatggt gccaggaacg gcagcagcgg tcacaggcaa
    22021 gtaaatagta atgccggagg caagtttcct ccggctttat catgtcaccc actgtggtat
    22081 atgcgttgcg gtctgccaac tttgccgtga acaatttcag caataatcag atggcggctg
    22141 gcgcaatatt caagataacg cctggcagtg gtgcgactga tggtcagtgc ctgcgccacc
    22201 gtttctgccg tatgttgcac accaggctct ttaaacagtt ttcgcaccgc gtttagcgtc
    22261 aagggatcaa tgccggtcgg cagttcgtct ttaggttcac cgcgagcata ggcattaaac
    22321 atctcatcaa tttgcttctg gctggcgcta tcaatacttt ccagcatatg tttacgctgg
    22381 cggaaacggg ttagcgtttg ccccagccgt tcataggcaa tgggcttaat gagataatca
    22441 aatacaccac aacgtacggc ttcagacacc gtttccatat cgctggctgc agtggtaaac
    22501 accacgtcgc cgggataatg cgcctgcacc agttcatgca ataaattaat ccctctgccg
    22561 tcaggaagat agttatcgag caagattagc cccggcttaa aacgctcgat catcattcgg
    22621 gcctgcgcca ggtttcccgc cagtaatatc tgactgaatc cgggaatgtg acgaatatat
    22681 tccgcatgca tctctgccag cggcgtttcg tcctcaacga tcaatagggt taatggagct
    22741 gtcattaggt ttcactttcg gaatatagat tgaaaacaag gtaccgcagg gatcattatc
    22801 ttcgagagtg ataacaccac cgcagcgcgt tacgtagctg gcaatcaagt acaacccaat
    22861 gccatgttca ccgggctcgt cagcacgcgt actgaccccc tgctcaaata ttttgtctcg
    22921 tagagactct ggaacgccgc agccctgatc ggcgacttca atcaccacat catcgccttc
    22981 atcgctgagg aataattcaa cgatcttgtt tccttcatcg ctacgcaggc tggcttcgaa
    23041 ggcgttatca agtaaattgc ccacaatggc tgcaaactcg gtgctatcca gtcctggcgg
    23101 cagttgcgaa agctggctac cggggacaat gatcattttt agccccagtt cccgggcgcg
    23161 ctgcacttta ccaaaaagca gccccgccac ctggcgatcg gcaaacgcct cacgcaggct
    23221 gtcaataagc tgttgctggg cctgagactc cccctgcacc atcgccagca cgcgatcata
    23281 ctctttcatc tgcaacagac cattgagcgt cgacatccaa ttgagatgct cgtgtcgcaa
    23341 tgtacgaagg ctctcgacgt attgttttat ttgcgtcagt tgcgcattga gggtggaaat
    23401 ttcgtcttta ctacgaaagc tgataatggc ccccagcaaa tcatcacctg aacgaatagc
    23461 ttcccgattg gcaataacgc tcagaccgtt aaagttagcc accacatcct gacgtttttc
    23521 atcaatctgt tcggtaaaga aatcggcggg cctgaccact tcagcaatgg gtttacccaa
    23581 ccattgccgt ccgggagagc tcagccccag catctttctt gcgttacgat tgatggcggt
    23641 aatgtaacca tgcggatcca ccgcaatcag cccttcataa accgaactaa acagcgcctc
    23701 ttgctgacgg accacgcgtg cgatttgctt tggctccatg cccatcattt gccgacggat
    23761 atgcgctgcc aggaaccacg acagcaacat cagaatccct aacagcacga caaacacgcc
    23821 tgccatcggt aataaaaact cagcccgcca gctatcgatt ttactcacca ggtagccaat
    23881 cgacaccacg ccgatgactt ttccatcgtc atcaaagatt ggcgttttgg cgcgcatcgc
    23941 catccccatt gaccctttcc cggtaatgaa gtagctctcc cctttctcca gcgcgccctg
    24001 tttggtgaac tgcataggat aaccaatttt ctccggatta ggatggtaaa ggcggatcga
    24061 gtgccggtcc ccaatcacca cataatcaaa atcggtatct ctttgtaatt tgttagcgat
    24121 ggtcgccagc cgtttgtagt cacgcgtttt caccgcagag atgacactgt cattggaggc
    24181 aataattttc gcctgattca ttgccatgtc gcgtacatga agcgttaaat agtcctcaaa
    24241 actggccgta aaatattgcg ccagggctgc aatgacaaat attgagaaca ccagaatcag
    24301 caaaaagata cgcagcggaa atgccagtct ttggaaaaat gcaaactgtt tattctcgtt
    24361 aagctgcaac attgttttcc ttgcgggttg tcccgagcct ctttgacgga tgtaatta
//
LOCUS       DAZGSP010000075        24330 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZGSP010000075 DAZGSP010000000
VERSION     DAZGSP010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.075"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            132..893
                     /gene="adiY"
                     /locus_tag="ACHK74_003346"
     CDS             132..893
                     /gene="adiY"
                     /locus_tag="ACHK74_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFP0222107.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            1030..2367
                     /gene="adiC"
                     /locus_tag="ACHK74_003347"
     CDS             1030..2367
                     /gene="adiC"
                     /locus_tag="ACHK74_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFP0222108.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDVPISKD"
     gene            2471..4114
                     /gene="eptA"
                     /locus_tag="ACHK74_003348"
     CDS             2471..4114
                     /gene="eptA"
                     /locus_tag="ACHK74_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFP0222109.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4111..4779
                     /gene="basR"
                     /locus_tag="ACHK74_003349"
     CDS             4111..4779
                     /gene="basR"
                     /locus_tag="ACHK74_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFP0222110.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4780..5880
                     /gene="pmrB"
                     /locus_tag="ACHK74_003350"
     CDS             4780..5880
                     /gene="pmrB"
                     /locus_tag="ACHK74_003350"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFP0222111.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5856..5945)
                     /gene="pmrR"
                     /locus_tag="ACHK74_003351"
     CDS             complement(5856..5945)
                     /gene="pmrR"
                     /locus_tag="ACHK74_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFP0222112.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6057..7559)
                     /gene="proP"
                     /locus_tag="ACHK74_003352"
     CDS             complement(6057..7559)
                     /gene="proP"
                     /locus_tag="ACHK74_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFP0222113.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7823..8701)
                     /gene="rdcB"
                     /locus_tag="ACHK74_003353"
     CDS             complement(7823..8701)
                     /gene="rdcB"
                     /locus_tag="ACHK74_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFP0222114.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            complement(8698..10926)
                     /gene="crfC"
                     /locus_tag="ACHK74_003354"
     CDS             complement(8698..10926)
                     /gene="crfC"
                     /locus_tag="ACHK74_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFP0222115.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHDDLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQARGYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEVKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            11329..11664
                     /gene="yjdM"
                     /locus_tag="ACHK74_003355"
     CDS             11329..11664
                     /gene="yjdM"
                     /locus_tag="ACHK74_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFP0222116.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            11824..12267
                     /gene="yjdN"
                     /locus_tag="ACHK74_003356"
     CDS             11824..12267
                     /gene="yjdN"
                     /locus_tag="ACHK74_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFP0222117.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            12400..13188
                     /gene="phnC"
                     /locus_tag="ACHK74_003357"
     CDS             12400..13188
                     /gene="phnC"
                     /locus_tag="ACHK74_003357"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFP0222118.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            13213..14229
                     /gene="phnD"
                     /locus_tag="ACHK74_003358"
     CDS             13213..14229
                     /gene="phnD"
                     /locus_tag="ACHK74_003358"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0222119.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            14290..15069
                     /gene="phnE"
                     /locus_tag="ACHK74_003359"
     CDS             14290..15069
                     /gene="phnE"
                     /locus_tag="ACHK74_003359"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFP0222120.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            15090..15815
                     /gene="phnF"
                     /locus_tag="ACHK74_003360"
     CDS             15090..15815
                     /gene="phnF"
                     /locus_tag="ACHK74_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFP0222121.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            15816..16268
                     /gene="phnG"
                     /locus_tag="ACHK74_003361"
     CDS             15816..16268
                     /gene="phnG"
                     /locus_tag="ACHK74_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFP0222122.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            16265..16849
                     /gene="phnH"
                     /locus_tag="ACHK74_003362"
     CDS             16265..16849
                     /gene="phnH"
                     /locus_tag="ACHK74_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFP0222123.1"
                     /translation="MTLETAFILPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILYE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            16849..17913
                     /gene="phnI"
                     /locus_tag="ACHK74_003363"
     CDS             16849..17913
                     /gene="phnI"
                     /locus_tag="ACHK74_003363"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFP0222124.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTAHSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            17906..18751
                     /gene="phnJ"
                     /locus_tag="ACHK74_003364"
     CDS             17906..18751
                     /gene="phnJ"
                     /locus_tag="ACHK74_003364"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFP0222125.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            18748..19506
                     /gene="phnK"
                     /locus_tag="ACHK74_003365"
     CDS             18748..19506
                     /gene="phnK"
                     /locus_tag="ACHK74_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFP0222126.1"
                     /translation="MNKPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            19617..20297
                     /gene="phnL"
                     /locus_tag="ACHK74_003366"
     CDS             19617..20297
                     /gene="phnL"
                     /locus_tag="ACHK74_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFP0222127.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            20294..21430
                     /gene="phnM"
                     /locus_tag="ACHK74_003367"
     CDS             20294..21430
                     /gene="phnM"
                     /locus_tag="ACHK74_003367"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFP0222128.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            21430..21987
                     /gene="phnN"
                     /locus_tag="ACHK74_003368"
     CDS             21430..21987
                     /gene="phnN"
                     /locus_tag="ACHK74_003368"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFP0222129.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINTRLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            21974..22408
                     /gene="phnO"
                     /locus_tag="ACHK74_003369"
     CDS             21974..22408
                     /gene="phnO"
                     /locus_tag="ACHK74_003369"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFP0222130.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            22410..23168
                     /gene="phnP"
                     /locus_tag="ACHK74_003370"
     CDS             22410..23168
                     /gene="phnP"
                     /locus_tag="ACHK74_003370"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFP0222131.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(23315..23644)
                     /gene="yjdP"
                     /locus_tag="ACHK74_003371"
     CDS             complement(23315..23644)
                     /gene="yjdP"
                     /locus_tag="ACHK74_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFP0222132.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(23713..24162)
                     /gene="rpiB"
                     /locus_tag="ACHK74_003372"
                     /gene_synonym="alsI"
     CDS             complement(23713..24162)
                     /gene="rpiB"
                     /locus_tag="ACHK74_003372"
                     /gene_synonym="alsI"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HFP0222133.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEVDGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
     gene            24147..>24330
                     /locus_tag="ACHK74_003373"
     CDS             24147..>24330
                     /locus_tag="ACHK74_003373"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222134.1"
                     /translation="MQSFSFIFPVRAKTSQSKNICDVNELKTPKIDEIRLPDLFYRVT
                     SDSAIALKTLTIQQHLS"
BASE COUNT         5730 a         6856 c         6384 g         5360 t
ORIGIN      
        1 aaatttgtaa tattagtaaa actcgttatt tttatgcatg tttatattca tcatacaatt
       61 atataaccat ttcccggtat cgctttgctt tagcgagaac tggtctttta tttgcactca
      121 ggagtacatg tatgaggatt tgcagcgacc aaccttgtat tgttttattg actgaaaaag
      181 atgtctggat aagggtgaat gggaaagaac ctattagcct taaagctaac catatggcgt
      241 tattaaattg tgaaaataat attatcgacg tctcctctct taacaacact ttggttgctc
      301 atattagtca cgacatcatc aaagattacc tccggtttct gaataaagat ctctcgcaaa
      361 taccagtatg gcaacgtagc gctacgccca tactcaccct gccatgcctg acgccagacg
      421 tctttcgcgt tgccgcgcaa cacagcatga tgcccgcaga aactgagtca gaaaaggaac
      481 gaacacgcgc attattattc acggtgctat cccgttttct cgacagtaaa aaattccttt
      541 cattaatgat gtatatgtta cgtaattgtg taagtgacag cgtttatcaa attattgaaa
      601 gcgatattca caaagactgg aatcttagta tggtagccag ttgtttatgt cttagcccaa
      661 gtctgttaaa gaaaaagttg aaaagcgaaa acaccagtta tagccaaata atcaccacct
      721 gccgcatgcg ttatgccgta aatgaattaa tgatggacgg taaaaatatc tctcaggtat
      781 cacagtcctg cggctacaac agtacgtcgt actttatttc tgtctttaaa gacttctacg
      841 gtatgacgcc gctgcattat gttagtcagc acagagaacg cactgtcgcc tgatttttaa
      901 ccttaacgaa gagctatatt aataacggca tcagcgataa cccggtcgat aataattcaa
      961 ctatcgaatg caggcgtatg atatgacgta atttattgtc acgaagctcg ccttcgcagg
     1021 agtttaatta tgtcttcgga tgctgatgct cacaaagtgg gcttaatccc cgtcaccctg
     1081 atggtgtcgg ggaatattat ggggtcaggt gtttttctgt tacctgcaaa cctggcctct
     1141 actggcggga ttgccattta tggatggttg gtgacgatta tcggtgcgct ggggctctcg
     1201 atggtatacg ccaaaatgtc gttcctcgat ccaagtcctg gtggttctta cgcgtacgcc
     1261 cgccgctgct ttggcccgtt tctcggttat caaaccaacg tcctctactg gctggcctgc
     1321 tggatcggca atatcgccat ggtggtcatt ggcgtaggat atttaagtta cttcttcccg
     1381 attctgaaag atccattggt attaaccatc acctgcgtcg tggtgctgtg gatcttcgtc
     1441 ctgctgaaca ttgtcggtcc gaaaatgatc acccgtgtgc aggccgttgc caccgtgctg
     1501 gcgctgatcc ccatcgtcgg gattgccgta tttggctggt tctggttccg tggtgaaacc
     1561 tatatggcgg catggaacgt cagcggcctg ggcaccttcg gtgcaattca aagtaccctt
     1621 aacgttacgc tgtggtcgtt catcggtgtg gaaagtgcct ccgttgccgc aggtgtggtg
     1681 aaaaacccga aacgcaatgt ccctatcgcc accattggtg gggtattgat tgccgccgtt
     1741 tgctatgtac tttctaccac cgcgattatg gggatgatcc ctaatgccgc actgcgcgtt
     1801 tctgcttcgc cattcggtga tgccgcacgg atggcgttgg gtgacaccgc cggggccatt
     1861 gtttccttct gcgcagctgc gggttgctta ggttcactgg gcggctggac gttgctggcg
     1921 ggtcaaacgg cgaaagccgc tgccgatgac ggactgttcc caccgatttt tgcccgtgta
     1981 aataaagcgg gcacgccagt ggcggggctg attatcgtcg gtattttgat gaccatcttc
     2041 cagctcagca gcatttcacc aaacgcgacc aaagagttcg gtctggtttc ttccgtctcg
     2101 gtcatcttta cactggtgcc atatctttac acctgtgcgg cgttactgct gctcggtcac
     2161 ggtcactttg gtaaagcacg cccggcatat ctggctgtta ctaccattgc cttcctctac
     2221 tgcatttggg ccgtggtggg gtccggggcg aaagaggtta tgtggtcatt tgtcaccctg
     2281 atggtcatca ccgccatgta tgcgctgaat tacaaccggc tgcataaaaa cccgtatccc
     2341 ttagatgtac caataagcaa agattaattc cccttaatcc agcaaacata aaagccaacc
     2401 ttaagaactt aaggttggct taattttgct ttgcgagcat atgcgcactt tgttcgatgg
     2461 aaacaccgtg atgttgaagc gcctactaaa aagaccctct ttgaatttac tcgcctggct
     2521 attgttggcc gctttttata tctctatctg cctgaatatt gcctttttta aacaggtgtt
     2581 gcaggcgctg ccgctggatt cgctgcataa cgtactggtt ttcttgtcga tgccggtcgt
     2641 cgctttcagc gtgattaata ttgtcctgac actaagctct ttcttatggc ttaatcgacc
     2701 actggcctgc ctgtttattc tggttggcgc ggctgcacaa tatttcataa tgacttacgg
     2761 catcgtcatc gaccgctcga tgattgccaa tattattgat accactccgg cagaaagtta
     2821 tgcgctgatg acaccgcaaa tgttattaac gctgggattc agcggcgtgc ttgctgcgct
     2881 gattgcctgc tggataaaaa tcaaacctgc cacctcgcgt ctgcgcagtg ttcttttccg
     2941 tggagccaat attctggttt ctgtactact gattttgctg gtcgccgcac tgttttataa
     3001 agactacgcc tcgttgttcc gcaataacaa agagctggtg aaatccttaa gcccctctaa
     3061 cagcattgtt gccagctggt catggtactc ccatcagcga ctagcaaatc taccgctggt
     3121 gcgaattggt gaagacgccc accgcaaccc gttaatgcag aacgaaaaac gtaaaaattt
     3181 gaccatcctg attgtcggcg aaacctcgcg ggcggagaac ttctccctca acggctaccc
     3241 gcgtgaaact aacccgcggc tggcgaaaga taacgtggtc tatttcccta ataccgcatc
     3301 ttgcggcacg gcaacggcag tttcagtacc gtgcatgttc tcggatatgc cgcgtgagca
     3361 ttacaaagaa gagctggcac agcaccagga aggcgtgctg gatatcattc agcgagcggg
     3421 catcaacgtg ctgtggaatg acaacgatgg cggctgtaaa ggtgcctgcg accgcgtgcc
     3481 tcaccagaac gtcaccgcgc tgaatctgcc tggtcagtgc atcaacggcg aatgctatga
     3541 cgaagtgctg ttccacgggc tggaagagta catcaataac ctgcagggtg atggcgtgat
     3601 tgtcttacac accatcggca gccacggtcc gacctattac aaccgctatc cgccgcagtt
     3661 caggaaattt accccaacct gcgacaccaa tgagatccag acctgtagca aagagcaact
     3721 ggtgaacact tacgacaaca cgctggttta cgtcgactat attgttgata aagcgattaa
     3781 tctgctgaaa gaacatcagg ataaatttac caccagcctg gtttatcttt ctgaccacgg
     3841 tgaatcgtta ggtgaaaatg gcatctatct gcacggtctg ccttatgcca tcgccccgga
     3901 tagccaaaaa caggtgccga tgctgctgtg gctgtcggag gattatcaaa aacggtatca
     3961 ggttgaccag aactgcctgc aaaaacaggc gcaaacgcaa cactattcac aagacaattt
     4021 attctccacg ctattgggat taactggcgt tgagacgaag tattaccagg ctgcggatga
     4081 tattctgcaa acttgcagga gagtgagtga atgaaaattc tgattgttga agacgatacg
     4141 ctgttattgc agggattgat tctggcggcg caaactgaag gctacgcgtg cgatggcgtg
     4201 acaaccgcgc ggatggcgga acaaagcctt gaggccggtc attacagcct ggtggtactg
     4261 gatttagggt tacccgacga agatggactg cattttctcg cccgtatccg gcagaaaaaa
     4321 tacaccctgc cagtactgat cctcaccgct cgcgatacgc tgaccgacaa aatcgctggg
     4381 ctggatgtcg gtgccgatga ctatctggtg aagccttttg cgctggaaga gttacacgcc
     4441 cgtatccgcg ccctgctacg acgccataat aatcagggcg aaagtgagct gattgttggc
     4501 aatctgacgc tgaatatggg tcgccgtcag gtatggatgg gcggtgaaga gttgattctg
     4561 acgcccaaag aatatgctct gctgtcacgg ttaatgctca aagcaggcag tccggtgcat
     4621 cgggaaattc tctacaacga catctataac tgggacaatg aaccctcgac caacaccctg
     4681 gaagtgcata tccacaatct gcgcgacaaa gtgggcaaag cccgtatccg caccgtgcgc
     4741 ggctttggct atatgctggt cgcgaatgag gaaaactaat tgaatctgat gcattttctg
     4801 cgccgaccaa tatcgctgcg ccaacggctg atattgacca tcggggccat tttgttggtg
     4861 tttgagctga tcagcgtctt ctggctatgg catgaaagta ccgagcagat tcagctgttt
     4921 gagcaggcgc tacgcgacaa tcgcaacaac gatcgccaca tcatgcgtga gatccgcgag
     4981 gcggtcgcca gcctgattgt ccccggcgtc tttatggtca gcctgacgct atttatctgc
     5041 tatcaggcgg tacgccgcat cactcgcccg ctggcggagc tgcaaaaaga gctggaagcg
     5101 cgcaccgccg acaacttaac gcccattgcc attcacagcg ccaccctcga aatcgaagcg
     5161 gtggtttcgg cgttgaacga tctggtcagt cgcctgacca gcacgctgga taacgaaagg
     5221 ttgtttaccg ctgacgtcgc gcacgaactg cgaacgccac tggcgggggt gcgtttgcat
     5281 ctggaactgc tggcgaaaac gcatcacatt gatgtagcac cgttagtggc acggcttgat
     5341 cagatgatgg agagcgtctc ccagctgctg caactggcgc gtgccggaca gtcattttct
     5401 tccggtaatt atcaacatgt aaaactgctg gaagatgtca ttctcccctc gtatgacgaa
     5461 ctcagtacca tgctcgacca gcgacagcaa accctgctat tgccagagag cgccgccgac
     5521 atcaccgttc agggtgatgc aaccctgctg cggatgttat tgcgaaacct ggtagaaaac
     5581 gcccatcgtt acagcccgca aggcagcaac attatgatta agctgcaaga agatggcgga
     5641 gcggtcatgg cagttgaaga tgaaggacca ggtattgatg agagtaaatg cggggagttg
     5701 agtaaagcgt ttgtacgtat ggacagccgt tatggcggga ttggtctggg gttaagtatt
     5761 gtcagccgca ttacacagtt gcatcacggg cagtttttcc tgcaaaaccg gcaagagact
     5821 tccggcacgc gggcctgggt acggctgaag aaagatcaga acgtggcaaa ccagatataa
     5881 agaaagctgc tgaccaccag cacgctgaac acggtagtta aactttcata aacacggttt
     5941 ttcattgcac tctcctccgt taacctggag gagagtatgc gcgtcagaga ttaagtgaac
     6001 cttaagagtt caattggcct gataagacag cgtcacatca ggccatccgt ttcagcttat
     6061 tcatcaattc gcggatgttg ctgcaccagg cgggtacgtt tcgcctgcaa atcggcaatc
     6121 tcgtggtcaa tattatcgat tttctgctcg atattatcgt aatgctcgac gagaatttcc
     6181 ttcgcttcct gtatatctga cgccgccggt gtcgcacctt tcaacggacg atttgccgtc
     6241 tctttcatgg ttacgccggt gattaaacca atcaccgcca ctaccatcag gtaataggca
     6301 ggcatcatca gattctgcga gctttcgacc agccaggccg ccagtgttgg cgtcagaccg
     6361 gcaaccagca ccgaaatatt aaatgccgcc gccagcgcgc tgtaacggat atgcgtcggg
     6421 aacatcgctg gcaaggtaga agccataacg cccgtaaagc agttaaggat caccgccagc
     6481 atcagtaacc cggcaaaaat caggccgatg acgttactgt taatcagaat aaacgccggg
     6541 atcgccaaca caaacagggc aacactacca agtagcacaa acggacgacg gccaaaacgg
     6601 tcactcagca agcccatcac cggctggaca aacagcatac cgatcataat ggcgataata
     6661 atcagcaccc cgtggtcttc ggagtaatgc aggttatgcg acaaataact cggcatatag
     6721 gtcagcaaca tgtagtaagt cacgttggtg gcaattacca gaccaataca tgtcaacagg
     6781 ctgcgccagt gtttggtggc aatctcttta aacgagactt tcgggccatc ctgcaaacct
     6841 tcgcggtcgc cctgttccag tttatcgaca tgctgctgga acgccggagt ttcttccagc
     6901 gcatggcgca ggtaaagccc gataatccct aacggcagag caataaagaa cggaatacgc
     6961 cagccccagt cgaggaagtt cgcttcgccg acaatggtcg aaattaacac cactacgcca
     7021 gcacccagca caaacccggc aatagaacca aagtccagcc agctgcccat aaagccacgt
     7081 ttacggtcag gggagtattc cgcaacaaat atcgacgccc cggtatattc accgccgacc
     7141 gagaaacctt gtgccatctt acagatcaac agcagaatcg gtgcccaaat accaatcgtg
     7201 tcgtaggacg gtattaagcc aatacagaac gtactgatcg acataatcac aatagtgata
     7261 gcgaggatct tctggcgacc atatttatcg cccaacatac caaagaagag tccgccaagc
     7321 ggtcgaatca gaaagggaac ggagaacgtg gcaagtgcag caaccatctg cacgctgggg
     7381 tcagcccccg ggaaaaaaac tttacctaat gcgtaagcaa caaaaccata aacaccaaaa
     7441 tcaaaccatt ccattgcatt acccagtgat gctgcggtaa tggctttacg cagtttaccg
     7501 tcatcaataa tggtgacatc acgaagggta atcggtttta cttttttcct tttcagcata
     7561 gctttcctcg cagagttggc ccatttccgc aaacgccaga aaaatcagcg cgacggccgc
     7621 aaaaggggag cgggaattgc aggatgcaat ctctgtaaca agcgtagcgg gtttgcttac
     7681 accctccggt taaggaatgt acaatcaaac cagttgacgc ctgaattggg cagttaatga
     7741 cctttttacc ctaccagtgt ttaaatttgt gatcaacttc acacatggat ttcgtttagg
     7801 actcattgat gtaactgatt atttacgcca aatttacaga gaagatttcc tcgaccattg
     7861 cccccgccag acgctgcgct gaacagagtc gtggcatccc cagcgcgaca gtttgccagc
     7921 gttgggtcac tgaccagcaa acgggttgtt tatctgccgt ggtccacttt cccagccaac
     7981 ttaaaaaatc agcatgatcc ccgcccgtcg gaacggtcga agaggcaaac cactgatggt
     8041 aatagtgacg cgtggtcggg gccgcattga tactctctgc cagatgttgt gtcgctttca
     8101 cgcgcaaatc acccagcaac aacgccaccg cttgcgggct tcccagacga caggcatcgc
     8161 caaatgcccc ccaacgcaac tcttcgagaa cgataaaaca gcgcccaggc aacgaccagg
     8221 cgttatacgc cccagcacgc cagcgggtaa aaatttgctc gctctgttca cccgcctgca
     8281 ccagtaggcc tcgttgcgtt aatgcctttt ccttccagcg acaacgggct gaaaaccact
     8341 catacaaggc ggcaatttgc tgggggagct ccgggaaagc agagtgctgg cggtgatcca
     8401 ttttttgcag cagcgccaga tgctgcgcga tctgcgtcag tgccagatga ccgggatcca
     8461 gcatcgacgc cagtttttcc accaatgtca gacgcaggct aagcggcgca tttaacatgc
     8521 cgccgttcgt ccatgtctga agctgcacgc gcgcaagaag ttcctgcatc agtcgttcac
     8581 gaaactgctg ctgatgggct tgtggaaggc gggcatcatc gccctgcgcc agatccacta
     8641 aaaagcgggg ataaaccgac tccagcacgc gaccggggcc gtcaagtaac gtcttggtca
     8701 atatcgttct gccgtgaaaa gtgtttgaat atcatcgcgt aacagctggg cgtcggtgta
     8761 aatccagccg tgagtcatca cagtctgctg caattgctgc tgcatcagcc tgaccaccga
     8821 ttcattttgt tgacgcagag ccagactttc gcgtaaacgc gtctgtaatt ccgtcaaaca
     8881 tgaagcgaac tcagcgaaaa aagtattcat gcctgccgta acagattcat cgacctgctc
     8941 tgccagaact ttagccattt gttggcaata aagatcgact tctgcgctta aagctcgttg
     9001 caacacactg taatcaaccg tttctgtcgg ggatttctca tttccccgtc cccagtcggg
     9061 ctgattcaac cagcgcgaaa aagtctcacg cacaacgcct aaacgcgtgc tctgctcgtc
     9121 cgttgcttcc tggcgcgaaa tgacttcact gaacagctgg cgagtgttga agtggggaac
     9181 tacgccgtga aaaacaggaa aatgaaaccc aggacgaaac cctgactcgc tcaattccat
     9241 tttgacttgt tgctcaatgg gccgaataac atcggttaac acccggcaaa gggtggattc
     9301 aagctcggca aaacgcagcg taaagtcgcg actgatggtg ttctgcgccg tctgtaacag
     9361 tgtctcacag caggtacgaa tctcgcttaa cggctccgaa tcatcctgaa acaaggcggc
     9421 taactgcgca ttcagcgcat cttgctgttg acgcagaaag tggttggcgg aggtcagggc
     9481 cagctcgatt tcatgtttaa cctcgccgct cacctgcgcc tgattgagtt gcaatagctg
     9541 caaactttct tcgacctgat ggatattttg ccgcaattgt tcacaagcga cgtttaaccc
     9601 gtgcgcacga aaatccaggt atccccgcgc ctgctgcgcg taattcaaca gtttatgcgc
     9661 agcagatcgc agagcataca acgaggcgtt agcgtaagcg gcatgaagca acgcctgaat
     9721 tggctgggcg aacagcgaat cttcccacaa ctgatcggca gcatgacgaa tatgttcgag
     9781 gtccgccaga tcgtcatgac gccagcgcct gccgagcgca gcatgggcaa aatcttccac
     9841 ccagcgttgt tgctctggcg ctggtaactt accgttgttg gctaactcat ggcgcgctcg
     9901 attcgccagg tagccccaca tcgacgacac cggaaatatc tgctgtggcg taatacagcc
     9961 tttcatcagc gtcccggaaa tcagtgcccg cacctggtcg gcgtcgtcac tgttacgatc
    10021 ctgttgatcg aacttattga ccagcacata cagcggcacc gattgcccca ccgccaaaat
    10081 cgcctcacgg acctcttcat cggagatcga tttcagttgc gtataatcca gcaccgccag
    10141 taccgccgag gcgcgtgcca gctgctggtt aagcattttt tgcagatgcg gttgcccggc
    10201 ttcatttggc ccgggggtat ccagtaacgt caactgaccg ggataactct ccagccccgc
    10261 cagatggaca aactccactt caatcacggg aatatgctca atggcggcgt aagcagaaaa
    10321 aggaaaatcg acgtccagcg ccttcgccag tcgcactaaa tcattcaaac ttttcagaca
    10381 atgaaaaata ggctgggcac ccagataata tttttcgaaa gcgacgccat tttcgatccg
    10441 ctgcataagc gcacgcatat ctttatctat ttccagcaca tcggtcagat gcttaatatc
    10501 gcaatcacgc aggcgctgtt gtaattgttg aattaaacaa tcgattggcg cgacatgtga
    10561 aaaatgcagt accggttcct tttgcccggg cgtatggcga ataagcgtcg gcagcgcagt
    10621 cattgggcga ttacgattag gcagaacctc cgtaccaaca atggcattaa tggtggttga
    10681 tttccctgct ttcatggtac cgacaattgc cagcaccatt tccagtcggg aaattttacg
    10741 caactcatta ttcagcatcg cgtgacgttc ggcgatatta ggctgactcc agggtaaagc
    10801 cagttgtggc gcgtcgtctc ctggtacaga gagaggcatt ttttccagta actgcaactg
    10861 ttggcgagaa agctgtaaca ggcgttcagc ctcctgactt aactcataca gggtctgtgt
    10921 gtacatagaa aattcttcct taaagcaaat tttgttactt ttatttagcc agattgtttt
    10981 tgagttctgt tttcggcttt tataattact gcaagaaata attttatatt tagtgtgttg
    11041 ttttttatca aaataaataa cgtcttctga tgcgtttaaa acgtcagaaa gataaaaata
    11101 tcatgtgaat aaaaaaaaag aacaagtaga gcattaacat tatcttaaat aataaataga
    11161 ggcaaaaaga ttattttctt tttgcgtttc ctttcaaatg aaaacgatcg tcgtctaaaa
    11221 tcagcagtac ccccgacaaa ctcagggatt ttgtgtataa ttgcggcctt tttcggcaat
    11281 ctgccgtttt ttggcgcttt tgccctgctg acttttgagg aaatccacat gtcattacca
    11341 cactgcccaa aatgcaactc cgaatacact tacgaagata acggcatgta catctgcccg
    11401 gaatgtgcct acgaatggaa cgacgcagaa cctgcacagg aaagcgacga gctgatcgtt
    11461 aaagatgcta acggcaatct gctggctgac ggcgacagcg ttaccatcat taaagatctg
    11521 aaggtgaaag gtagctcttc gatgctgaaa attggcacca aagtgaaaaa catccgcctg
    11581 gttgaaggcg accataacat cgattgcaaa atcgacggtt ttggtccgat gaaactgaaa
    11641 tctgagtttg tgaaaaagaa ctgattgtat tgtgatcggt aagccggata aggcgctcgc
    11701 gccgcatccg gcaacgatgc cgactgcctg atgcgacgct gaccgcgtct tatcaggctt
    11761 ccctctcatc tgtataaatt tcgaactaca cttaactggc ttctcttaac tgaggtcacc
    11821 atcatgccgt taagtcccta cctctctttt gccggtaact gttccgacgc gattgcctat
    11881 tatcaacgta cgttgggcgc tgaactactc tataaaatca gcttcggcga aatgccaaaa
    11941 tcagcgcagg acagcgccga gaactgccct tccggaatgc aatttcccga taccgccatc
    12001 gctcatgcca acgtgcgcat tgccggaagc gacatcatga tgagcgatgc catgccgtca
    12061 ggaaaagcca gctactccgg ctttacgctg gtgctcgatt cgcaacaggt cgaagaagga
    12121 aaacgctggt ttgacaatct tgccgctaac ggaaaaatcg aaatggcctg gcaggaaact
    12181 ttctgggcgc atggctttgg caaagtcacc gataaatttg gcgtaccgtg gatgattaat
    12241 gtcgtcaaac aacaaccaac gcaataatcc gccgggaggc ccgccctccc gcactgtcat
    12301 cgaattcccg ttaactcttc atctgttagt cacttttaat taaccaaatc gtcacaataa
    12361 tccgccacga tggagccact tttttaggga ggctgcatca tgcaaacgat tatccgtgtc
    12421 gagaagctcg ccaaaacctt caatcagcat caggcgctgc atgcggttga tctgaacatt
    12481 catcacggtg aaatggtggc tctgcttggg ccgtcgggtt ccggcaaatc caccctttta
    12541 cgtcacttaa gcggtttgat taccggcgat aaatccgccg gcagccatat cgagctgctg
    12601 ggccgcacag tccagcgcga aggccgtctg gcgcgcgata tccgcaaaag ccgcgccaac
    12661 accggctaca tcttccaaca attcaacctg gtgaaccgcc tgagcgtact ggagaacgtg
    12721 ctgattggcg cgctcggcag cacgccgttc tggcgcacct gttttagctg gttcaccggc
    12781 gagcagaaac agcgcgcgtt acaggcgctg acccgcgttg gcatggtgca ttttgcccat
    12841 cagcgcgttt ccaccctctc cggcggacag cagcagcgtg tggcgattgc ccgcgcgctg
    12901 atgcagcagg cgaaggtgat tctggccgat gaacccatcg cctcgctgga cccggaatcc
    12961 gcccgcatcg tgatggacac cctgcgcgac atcaatcaga acgacggcat caccgtggtc
    13021 gtcacgctgc atcaggtgga ttacgccctg cgctactgcg aacgcatcgt cgccctgcgc
    13081 caggggcacg ttttctacga cggcagcagc caacagtttg ataacgaacg ttttgaccat
    13141 ctctaccgca gcattaatcg catcgaagag aacgcgaaag ctgcctgaca tccccatcat
    13201 tgaggaaaac gaatgaacgc taagataatt gcctcgctgg ccttcaccag catgttcagc
    13261 ctcagcaccc tgttaagccc ggcacacgcc gaagagcagg aaaaggcgct gaatttcggc
    13321 attatttcaa cggaatcaca gcaaaacctg aaaccgcaat ggacgccatt cttacaggat
    13381 atggagaaga agctgggcgt gaaggtgaac gccttctttg ccccagacta cgcaggcatt
    13441 atccagggaa tgcgcttcaa taaagtggat atcgcctggt acggcaacct gtcggcaatg
    13501 gaagcggtgg atcgcgccaa cggccaggtc tttgcccaga cggtcgcggc ggatggatcg
    13561 ccgggttact ggagcgtgtt gatcgtcaac aaagacagcc cgatcaacaa cctgaacgat
    13621 ctgctggcga agcggaaaga tctcaccttc ggcaatggcg atcctaactc cacctctggc
    13681 ttcctcgtcc ccggttacta cgtctttgcc aaaaacaata tctccgccag cgactttaag
    13741 cgcaccgtca acgctgggca tgaaactaac gcgctggccg tcgccaacaa gcaggtggat
    13801 gttgccacca acaacaccga aaacctcgac aagctgaaaa cctccgcgcc agagaagctg
    13861 aaagaactga aggtgatctg gaagtcgccg ctgatcccag gcgatccgat cgtctggcgc
    13921 aagaatctct ccgaaaccac caaagacaag atctacgact tctttatgaa ctacggaaaa
    13981 acgccggaag agaaagcggt gctggaacgc ctgggctggg ccccgttccg cgcctccagc
    14041 gacctgcaac tggtgccgat tcgccagctc gcactgttta aagagatgca gggcgtaaaa
    14101 agcaataaag gactgaatga gcaggacaag ctggcgaaaa ccaccgagat tcaggcgcag
    14161 ctggatgacc tggaccgcct gaacaacgcg ttaagcgcga tgagttcggt gagtaaagcg
    14221 gtgcagtaat gtgctcgatc ggtcccctct cccacaggga gagggagaaa acattaaacg
    14281 gagccaaaca tgcaaaccat caccatcgcc ccacccaagc gtagctggtt ctcgcttctg
    14341 agctgggccg ttgttctcgc cgtgctggtc gtctcgtggc agggcgcgga aatggccccg
    14401 cttacgctga ttaaagacgg cggcaacatg gcaaccttcg ctgccgactt cttcccgccc
    14461 gatttcagcc agtggcagga ttacctcacc gaaatggccg tcacgctgca aatcgccgtc
    14521 tggggcaccg cgctggcggt ggttctctcc atcccctttg gcctgatgag cgccgaaaac
    14581 ctggtgccgt ggtgggttta ccagcccgtt cgccgcctga tggacgcctg ccgcgccatt
    14641 aacgaaatgg tcttcgccat gctgttcgtg gtcgccgtcg gtctcggacc gttcgctggc
    14701 gtgctggcgc tatttatcca caccaccggc gtgctctcca agctgctttc cgaggcggtg
    14761 gaagcgattg aacccggccc ggtggaaggc attcgcgcca ccggtgccaa caagctcgaa
    14821 gagatcctct acggcgtgct gccacaggtg atgccgctgc tgatctccta ctccctctat
    14881 cgcttcgaat ccaacgtccg ctcggcgacc gtcgtcggca tggtcggcgc aggcgggatc
    14941 ggcgtcaccc tgtgggaagc gattcgcggt ttccagttcc agcaaacctg cgccctgatg
    15001 gtgcttatca tcgtcacggt cagcctgctg gatttcctct ctcaacggtt gcgtaagcac
    15061 tttatctgat aagcgaggca ttgatatcta tgcacttgtc tacacatccg accagctacc
    15121 caacacgcta tcaggagata gccgcaaaac ttgagcagga gcttcgtcaa cactatcgct
    15181 gcggcgacta tcttcccgcc gagcagcagc tggcggcgcg ctttgaggta aatcgccaca
    15241 ccctgcgccg cgccatcgac caactggtgg aaaaaggctg ggtacagcgc cgtcagggcg
    15301 tcggcgtgct ggtgctgatg cgcccgttcg attacccgct caacgcccag gcgcgtttta
    15361 gccagaacct gctggatcag ggcagccatc ccaccagcga aaaactgctg tcggtgctgc
    15421 gcccggcatc tggtcacgtc gctgacgcgc tggggataac cgagggggag aacgtcatcc
    15481 acctgcgcac cctgcgccgg gtcaacggca tcgcgctctg tttaatcgac cactactttg
    15541 cggacctcac cctctggccg acactgcaac gtttcgacag cggttcgctg cacgattttc
    15601 tgcgcgagca aaccggaatt gcgctgcgcc gcagccagac gcggatcagc gcccgccgcg
    15661 cccaggccaa agagtgccag cgccttgaaa tcccgaatat gtcgccgctg ctgtgcgtgc
    15721 gcacccttaa ccaccgtgac ggtgaaagca gcccggcgga gtactccgtc agcctgacgc
    15781 gcgccgacat gattgaattc actatggagc actgaatgca cgcagatacc gcgacccgcc
    15841 agcactggat gtccgtactg gcgcacagcc aaccggctga actggcagcc cgcctgaacg
    15901 cgctgaacat caccgccgac tatgaagtga tccgcactgc cgaaactggc ctggtgcaga
    15961 ttcaggcgcg aatgggcggt accggcgaac gcttttttgc cggcgacgcc acgctgaccc
    16021 gcgccgccgt acgcctgact gacggcacgc tcggctacag ctgggtgctg gggcgcgaca
    16081 aacagcacgc cgaacgctgc gcgctgattg acgcgctgat gcagcaatct cgccactttc
    16141 aaaacttatc agaaactctt attgccccgc tggacgctga ccgtatggca cgcattgccg
    16201 cacgccaggc cgaagtgaac gccagccggg tcgacttctt tacgatggtt cgcggagata
    16261 acgcatgacc ctggaaaccg cttttattct tcctgtgcag gatgcccagc acagttttcg
    16321 tcgcctgtta aaggccatga gcgagccggg cgtgattgtc gccctgcatc agctcaaacg
    16381 cggctggcag ccgctgaata tcgccaccac cagcgtgctg ctgacgctgg ccgataacga
    16441 cacgccggtg tggctttccg cgccattgag taacgacatc gtcagccaga gcctgcgttt
    16501 tcataccaac gcgccgctgg tcagccagcc ggaacaggcg accttcgcgg taacggatga
    16561 ggcgatttcc agcgaacagc ttaacgccct gtccaccggc accgccgttg cgccggaagc
    16621 gggtgcaacg ctgattttac aggtcgccag cctgagcggc ggacgcatgt tacgtctcac
    16681 cggcgcgggc attgccgaag aacgaatgat cgccccgcag ctgccggagt gcattctgta
    16741 cgaactcacc gagcgcccgc acccgttccc gctcggcatc gacctgatcc tgacctgcgg
    16801 cgagcgcctg ctggctattc cgcgaaccac gcatgtggag gtgtgctgat gtacgttgcc
    16861 gtgaaagggg gcgaaaaggc gatcgatgcc gcccacgccc tgcaagagag ccgacgccgg
    16921 ggcgataccg atttgcctga actgagcgtc gcccagattg aacagcagct taacctcgca
    16981 gtagatcgcg tgatgaccga aggcggcatt gccgaccgcg aactggcggc gctggcgctg
    17041 aaacaggcca gcggcgataa cgtcgaagca attttcctgc tgcgcgccta ccgcaccacg
    17101 ctggcgaagc tcgcggtaag cgagccgctc gacaccacgg agatgcgtct cgaacggcgt
    17161 atttcagcgg tttataaaga tattcccggc ggccagttgc ttggcccaac ctacgactac
    17221 acccatcgcc tgctcgattt caccctgctg gcaaacggcg aagcgccgac gctgaccacc
    17281 gcccacagcg aacaacagcc gtcgccgcac gttttcagcc tgctggcgcg tcaggggctg
    17341 gcgaagtttg aagaggatag cggcgcacag ccggatgaca tcacccgcac gccgccggtt
    17401 tacccctgct cacgctcctc ccgtttacag cagttgatgc gcggcgacga aggctatttg
    17461 ctggcgctgg cctactccac ccaacgcggc tacggtcgca accacccgtt cgcaggcgag
    17521 atccgcagcg gctatatcga cgtatcgatt gtgccggaag agctgggatt tgcggtaaac
    17581 gtcggcgaac tactgatgac cgagtgtgaa atggtcaacg gttttatcga cccgccggat
    17641 gagccgccgc acttcacgcg cggctacggg ctggtattcg gcatgagcga gcgcaaagcg
    17701 atggcaatgg cgctggtcga tcgtgcgttg caggctccgg aatacggcga gcacgcgaca
    17761 ggcccggcgc aggatgaaga gtttgtgctg gcacatgccg acaacgtcga agccgcaggc
    17821 tttgtctcgc acctcaaact cccccactac gtcgatttcc aggccgaact ggagctactc
    17881 aaacgtctgc aacaggagaa gaaccatggc taatctgagc ggctacaact ttgcctacct
    17941 cgacgagcag accaaacgca tgatccgccg cgctatctta aaagcggtgg cgatccccgg
    18001 ttatcaggtg ccgtttggcg ggcgcgagat gccgatgcca tacggctggg gaaccggcgg
    18061 catacagctc accgccagcg tgattggcga aagcgacgtg ctgaaggtga ttgaccaggg
    18121 tgcggatgac accaccaacg ccgtgtcgat tcgcaacttc tttaagcgcg tgaccggggt
    18181 aaacaccact gaacgtacgg acgatgcgac gcttatccag acgcgtcacc gcatccccga
    18241 aacgccgctg accgaagatc agatcattat cttccaggtg ccaatccccg agccgctgcg
    18301 ctttatcgag ccgcgcgaaa cggaaacccg caccatgcac gcgctggagg agtacggcgt
    18361 gatgcaggtg aaactgtatg aagatattgc ccgcttcggt catatcgcca ctacctacgc
    18421 ctatccggtg aaggttaacg ggcgctacgt aatggacccg tcgccgatcc cgaaattcga
    18481 taacccgaaa atggacatga tgcccgccct gcaactgttc ggcgcggggc gcgagaagcg
    18541 catctatgcg gtgccgccgt ttacccgcgt ggaaagtctc gatttcgacg atcacccgtt
    18601 caccgttcag cagtgggatg agccatgcgc catctgcgga tcgacccaca gctatcttga
    18661 tgaagtggtg ctggatgacg ccggaaaccg catgtttgtc tgctccgata ccgattattg
    18721 ccgccaacag agcgaggcga aaagccaatg aataaaccgt tactttcggt caataacctg
    18781 acccaccttt acgcgccggg caaaggcttt agcgatgtct cttttgattt atggccggga
    18841 gaagtgctgg gcattgtcgg agaatccggc tccgggaaga ccacgctgct gaagtcgatc
    18901 tccgcgcgtc tgacgccgca gcagggggag attcactacg agaaccgttc gctgtatgca
    18961 atgagcgagg ccgaccgccg tcgcctgctg cgcaccgaat ggggcgtagt gcatcagcat
    19021 ccactcgacg gcctgcgccg tcaggtctcg gcaggcggca atatcggcga gcggctgatg
    19081 gcgaccgggg cgcgtcatta cggcgacatt cgcgccaccg cgcagaagtg gctggaagag
    19141 gtggaaattc ccgccaaccg gatcgacgac ctgccgacca ccttttccgg cggcatgcag
    19201 caacgtttgc agattgcccg caacctggtg acgcatccga agctggtgtt tatggatgaa
    19261 ccgaccggcg ggctggatgt gtcggtgcag gcccgcctgc tcgacctgct gcgcggcctg
    19321 gtggtggagc tgaacctggc ggtggtgatt gtcacccatg atttaggcgt tgcccgcctg
    19381 ctggcggacc gtttgctggt gatgaagcag gggcaagtgg tggagagtgg gttaaccgac
    19441 cgcgtgctcg acgacccgca tcatccgtat acacagctgc tggtgtcatc ggttttgcag
    19501 aattgagccg gtgccggatg cggcgtaaac gccttatccg gcctacaaat gcgctccccg
    19561 taggtcggat aagacgcgtc agcgtcgcat ccgacacccg aaccacgagg cgaaaaatga
    19621 ttaacgtaca aaacgtcagt aaaaccttca tcctgcacca gcaaaacggc gtgcgcctgc
    19681 ccgtcctcaa ccgcgcctcg ctcaccgtca acgcgggcga atgcgtggtg ctccacggtc
    19741 actccggcag cggcaaatca accctgctgc gctcgctgta cgccaactat ctgcccgatg
    19801 aaggtcaaat ccagatcaaa cacggtgacg agtgggtaga cctggtcacc gcgccagccc
    19861 gcaaagtggt ggagatccgc aaaaccaccg tcggctgggt gagccagttt ctgcgcgtca
    19921 tcccgcgtat ttcagcactg gaagtggtga tgcagccgct gctcgatacc ggcgttccgc
    19981 gtgaagcctg cgccgctaaa gccgcgcgtc tgctcacccg cctgaacgtg ccggaacgcc
    20041 tgtggcacct ggcgccatca accttttccg gtggcgaaca gcagcgcgtc aacatcgccc
    20101 gcggctttat cgtcgactac cccattctgc tgctcgacga acctaccgcc tcgctggacg
    20161 ccaaaaacag cgccgccgta gtcgagctga ttcgcgaagc caaaacccgt ggcgcggcca
    20221 tcgtcggcat cttccatgac gaagccgtac gtaatgacgt cgccgaccgc ttgcacccaa
    20281 tgggagcctc tgcatgatta tcaataacgt caaactggtg ctggaaaacg aagtggtaca
    20341 cggttcgctg gaggtgcagg atggcgaaat ccgcgccttt gccgaaagcc agagccgcct
    20401 gccagaagcg atggacggcg aaggtggctg gctactaccc ggcctgattg agctgcatac
    20461 cgacaacctc gataaattct tcaccccgcg cccgaaagtc gactggcccg cccattcggc
    20521 gatgagcagc cacgacgcgc tgatggtggc aagcggcatc accaccgtgc tggacgcggt
    20581 ggcgattggc gacgtgcgcg acggcggcga tcggctggag aatctggaga agatgatcaa
    20641 caccatcgaa gagacgcaga aacgcggcgt caaccgcgcc gagcaccgtc tgcacctgcg
    20701 ctgcgagctg ccgcatcaca ccacactgcc gctgtttgaa aaactggttc agcgcgaacc
    20761 ggtgacgctg gtgtcgctga tggaccactc accgggccag cgccagttcg ctaaccgcga
    20821 gaagtatcgc gaatattatc agggcaaata ctccctcacc gatgcgcaga tgcagcagta
    20881 cgaagaagag caattggcgc tcgccgcacg ctggtcgcag ccgaatcgcg aatccatcgc
    20941 cgccctgtgc cgcgcgcgac aaattgcgct cgccagccac gatgacgcca cgcacgccca
    21001 cgttgccgaa tcccaccaac ttggcagcgt gatcgccgaa tttcccacca cgttcgaagc
    21061 ggcggaagcc tcgcgtaagc atggcatgaa cgtgctgatg ggtgcgccaa atatcgtgcg
    21121 tggcggctcg cactccggca atgtggcggc cagtgaactg gcgcagcttg gtctgctgga
    21181 tatcctctct tccgactact acccagccag cctgctggat gcggcgttcc gcgtcgccga
    21241 tgacgagagc aaccgcttta cgctaccgca ggcggtgagg ctggtgacca aaaatccggc
    21301 gcaggcgctg aatcttcagg atcgcggggt gattggcgag ggtaaacgcg ctgacctggt
    21361 gctggcgcat cgccagggca atcacattca tatcgaccac gtctggcgtc agggtaaaag
    21421 ggtgttctga tgatgggaaa actgatttgg ttaatggggc cgtccggctc cgggaaagac
    21481 agcctgctgg cggaactccg cctgcgggaa caaacgcagt tactggtggc gcatcgctac
    21541 atcacgcgcg acgccagcgc cggaagtgaa aaccatatcg ccctgagtga gcgggagttt
    21601 tttacccgcg cggggcaaaa cctgctggcc ttaagctggc acgctaacgg tctgtattat
    21661 ggcgtcggca tcgagattga cctctggctg cacgctggat tcgacgtagt cgtcaacggt
    21721 tcacgcgcgc atttgccgca ggcacgggcg cgctatcaat cggcgctgct gcccgtctgt
    21781 ttacaggttt cgccggagat cctgcgccag cgcctggaaa accgtggccg tgaaaacgcc
    21841 agtgaaatta acacccgcct ggcgcgcgcc gcccgctata ctccacagga ttgccatacg
    21901 ctcaacaatg acggcagcct gcgccagtcg gtcgacacgc tgctgacgct gatccatcag
    21961 aaggagaaac accatgcctg cttgtgagct tcgcccggcc acgcagtacg ataccgacgc
    22021 ggtttacgcg ctgatttgtg agctaaaaca ggcggagttt gaccaccatg cgtttcgcgt
    22081 gggctttaac gccaacctgc gcgacccaaa catgcgctac catctggcgc tgctggatgg
    22141 cgaagttgtc ggcatgatcg gcctgcattt gcagtttcat ctgcatcatg tcaactggat
    22201 cggcgaaatt caggaactgg tggtgatgcc gcaggcgcgc ggtctgaacg tcggcagtaa
    22261 attactggca tgggcagaag aagaagcccg ccaggccggg gccgaaatga ccgagctttc
    22321 caccaacgtg aagcgccacg acgcacaccg tttttacctg cgtgaaggct acgagcagag
    22381 ccacttccgc ttcaccaagg cgctgtaaca tgagcctgac cctcacgctc accggcactg
    22441 gcggcgcaca gggcgttccg gcatggggct gcgagtgcgc ggcctgcgcc agagcgcggc
    22501 gctcgccgca gtatcgccgc cagccatgca gcggcgtagt gaagtttaac gacgcgatca
    22561 ccctgatcga cgccgggcgg cacgatctca ccgatcgctg gtcgcctgga tcgttccagc
    22621 agtttttgct gacgcactac catatggatc acgtccaggg gctatttccg ctacgctggg
    22681 gcgtgggcga tgtgatcccg gtctacggcc cgccggatga acagggctgc gacgatctgt
    22741 ttaaacatcc gggcctgctt gatttcagcc acacagtgga accgtttgtg gtgtttgatt
    22801 tgcaggggtt gcaggtcacg ccgctgccgc ttaaccactc aaaactgacc ttcggttatc
    22861 tgctggaaac ggcacatagc cgggtggcgt ggctgtctga caccgctggc ctgccggaaa
    22921 aaacgctgaa atttttactc aataatcatc cgcaggtgat ggtgattgat tgcagtcacc
    22981 cgccgcgcgc ggatgcaccg cgtaatcact gtgatttaaa taccgtgctt gcgctgaatc
    23041 aggttatccg ctcgccacag gtgattctga cccatatcag ccaccagttt gatgcgtggc
    23101 tgatggaaaa cgcactaccg tcagggtttg aggtggggtt tgatgggatg gagattgggg
    23161 tggcgtgatg agagggaatg tgcgcgctgg ccccctcacc ctaaccctct ccccagaggg
    23221 gcgaggggac cgattgtgct caatattgaa tattgcgctc gttttctccc tctccccatt
    23281 ggggtgaggg gcgatgcctg ctccataccc aacctcatcg cccatactca tcttccattc
    23341 tccgctcttc atcctccagt tgccgacgct cctgatcaag ctggcgctgg cgatcgtcca
    23401 gctgcctgcg gcgatcttca aactggcggc ggcggtcgtc atattgtctg cgccgatcgt
    23461 cgctcacttc acgctgccag ccgtcgtcgc gcgaatcttc atagtctcgc ccacggtcag
    23521 ggttataagc gtcattaatc gcctgctgaa tattaccaat agtatcgtcg ataatatcag
    23581 cctgggccgg aacggtggac agcgtgagca gggtgaataa aagaaatagc ggaaagcgtt
    23641 tcattagcca acctcaaaaa gaaactctat ccacattagt cattactcat ccatgcaagt
    23701 agtggatgaa tctcaatttc tccgctgctc tattgccgta atcgcctcca cgcgttgttg
    23761 atgacgaccg ccttcgtact gtgcgcccag ccacgcatcc acaatcattt ttgccagttc
    23821 gaggccaacc actcgtgaac caaaagccag cacgttggtg tcgttatgct gccgcgaaag
    23881 ttgcgcggaa taaggttcgc tacagacgac cgcgcgaatt ccggcaaact tgttcgccgc
    23941 tatcgaaata ccgacgccag taccacaaat caaaatcccg ccatcaacct ctccgccagc
    24001 aacagccagt gcgacttgac tggcgtaatg cggataatca gtacgctctg acgaccaggt
    24061 tcctttatca atcacttcaa cgccacgctc aactaaatgt gccactattt catgttttaa
    24121 aatgaaaccg acatgatcac agccaaatgc aatctttttc attcatcttc cccgttcgtg
    24181 caaaaacttc acaatcaaaa aacatttgtg atgtaaatga attaaaaaca cccaaaatcg
    24241 atgaaatccg ccttcctgat ctgttttaca gagttacatc tgacagcgca atagcgttaa
    24301 agacacttac tatccagcaa catctatcat
//
LOCUS       DAZGSP010000076        23608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZGSP010000076 DAZGSP010000000
VERSION     DAZGSP010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.076"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..283
                     /locus_tag="ACHK74_003374"
     CDS             <1..283
                     /locus_tag="ACHK74_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFP0222135.1"
                     /translation="PVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISE
                     SGEVVDMTAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            283..5553
                     /gene="traI"
                     /locus_tag="ACHK74_003375"
     CDS             283..5553
                     /gene="traI"
                     /locus_tag="ACHK74_003375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFP0222136.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMLTLKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVSRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKISLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKIPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVSDSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVKMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDVREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVMAVSGDSVTLSDGQ
                     QTRVIRPGQEQAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAISKAVQKGTAHDVLEPGADREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVWIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAEEKPDQPDGKTEQAVRAIAGQEQDRASSSERETALPESVLRESQRVQDAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5573..6319
                     /gene="traX"
                     /locus_tag="ACHK74_003376"
     CDS             5573..6319
                     /gene="traX"
                     /locus_tag="ACHK74_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFP0222137.1"
                     /translation="MTTDNTNTTRNDSLAARTGTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLEQTWMFLAGRGAFPLFALVWGLNLSRHANIRQSAINRLWGWAVIAQF
                     AYYVAGFPWYEGNILFAFAVAAQVLTWCETRSLWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALLACLLAVIPALNLASSDVAAVAGLVMTVLTVGLVSC
                     TGKSLPRFWPGDFFPVFYACHLTVLGVLAV"
     gene            6378..7238
                     /locus_tag="ACHK74_003377"
     CDS             6378..7238
                     /locus_tag="ACHK74_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0222138.1"
                     /translation="MKITDHKLPEGIALTFRVPEGNIKHPLILLCHGFCGIRNVLLPS
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQTEDIISVINWAEKQVCIDNQRIG
                     LWGTSLGGCHVFNAAAQDKRVKSIVSQLAFADGEVLVTGEMNELEKASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            7341..7898
                     /locus_tag="ACHK74_003378"
     CDS             7341..7898
                     /locus_tag="ACHK74_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFP0222139.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVFQRNIPLSHKKLSRALKAITRSESYLCAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELRAILAE"
     gene            8052..8255
                     /locus_tag="ACHK74_003379"
     CDS             8052..8255
                     /locus_tag="ACHK74_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222140.1"
                     /translation="MAVSSGLSEHSGIYRYQRVGRHAVRQEIRGFFREAAEAWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
     gene            8997..9458
                     /locus_tag="ACHK74_003380"
     CDS             8997..9458
                     /locus_tag="ACHK74_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFP0222141.1"
                     /translation="MKKYIPVVLFVFSCQVFSADIHGRVVRVLDGDTIDVMLSQHPVR
                     VRLVNIDAPEKKQDYGRWSEKIMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQEEARQQKRGLWADSAPVPPWVWRSRK"
     gene            9561..9944
                     /locus_tag="ACHK74_003381"
     CDS             9561..9944
                     /locus_tag="ACHK74_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222142.1"
                     /translation="MNYQAVSELITSSNHNVLIVGDSASEVDSFLNKLNITDYKYYDF
                     SQIYSCSDRTLNDYAVIFIRDALNASEHIIIFNCTGWPDLNNESAVMQFARVARKSGK
                     QLIVAVREQDMKKMEAEFGRIIKIH"
     gene            10224..10373
                     /locus_tag="ACHK74_003382"
     CDS             10224..10373
                     /locus_tag="ACHK74_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0222143.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            10657..10917
                     /locus_tag="ACHK74_003383"
     CDS             10657..10917
                     /locus_tag="ACHK74_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFP0222144.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            11142..11216
                     /gene="tap"
                     /locus_tag="ACHK74_003384"
     CDS             11142..11216
                     /gene="tap"
                     /locus_tag="ACHK74_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFP0222145.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            11209..12066
                     /gene="repA"
                     /locus_tag="ACHK74_003385"
     CDS             11209..12066
                     /gene="repA"
                     /locus_tag="ACHK74_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFP0222146.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVVWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSHGMK
                     RARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
     gene            12767..12960
                     /locus_tag="ACHK74_003386"
                     /pseudo
     CDS             12767..12960
                     /locus_tag="ACHK74_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335629.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13003..13332
                     /locus_tag="ACHK74_003387"
     CDS             13003..13332
                     /locus_tag="ACHK74_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0222147.1"
                     /translation="MFSVVYHPEAREEATALPIKIRVKFDRLISKLEYDARLLREPDT
                     KPLGDGLFEIRTMGTDIARGIWVYHKGNTIIMLRVFIKKSQKTPAKEIDLAKKRLAEV
                     LNETGKP"
     gene            13313..13594
                     /locus_tag="ACHK74_003388"
     CDS             13313..13594
                     /locus_tag="ACHK74_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0222148.1"
                     /translation="MKLVSHKELHNEWMQDDEYRAAWQEEERKEKLRELLAEWRKHDN
                     LTKAQVAERMGVTPPVISRLENNITKASIDTLTRYAHACGIKKPVITLY"
     gene            13777..14061
                     /locus_tag="ACHK74_003389"
     CDS             13777..14061
                     /locus_tag="ACHK74_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFP0222149.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVISGQIIDHQAVQAWADSLSTDNPLPVPR"
     gene            14061..14336
                     /locus_tag="ACHK74_003390"
     CDS             14061..14336
                     /locus_tag="ACHK74_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0222150.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARMVQSLIQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            complement(14602..15098)
                     /locus_tag="ACHK74_003391"
                     /pseudo
     CDS             complement(14602..15098)
                     /locus_tag="ACHK74_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            15375..16250
                     /locus_tag="ACHK74_003392"
     CDS             15375..16250
                     /locus_tag="ACHK74_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFP0222151.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIIAMHEGNLESQQLTSMLSVQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLAAGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            16258..17034
                     /locus_tag="ACHK74_003393"
     CDS             16258..17034
                     /locus_tag="ACHK74_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFP0222152.1"
                     /translation="MMNILHFPQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSYATIEIT
                     RKKKSSDGEKRKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVSFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            17203..19464
                     /locus_tag="ACHK74_003394"
     CDS             17203..19464
                     /locus_tag="ACHK74_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFP0222153.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSVNMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGHKPACDSRVICVPQGSYDIDD
                     KVGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLVVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMAEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            19533..20708
                     /gene="senB"
                     /locus_tag="ACHK74_003395"
     CDS             19533..20708
                     /gene="senB"
                     /locus_tag="ACHK74_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFP0222154.1"
                     /translation="MNIFTLSKTPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHLRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSGDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(21338..21796)
                     /locus_tag="ACHK74_003396"
                     /pseudo
     CDS             complement(21338..21796)
                     /locus_tag="ACHK74_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612075.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(22236..22622)
                     /locus_tag="ACHK74_003397"
     CDS             complement(22236..22622)
                     /locus_tag="ACHK74_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFP0222155.1"
                     /translation="MKFVSSQEMIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYH
                     YEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVAQVFLKRNGIHIISSRISFDEMQ
                     IIALNAATGEYNWKMVSDHLKAIILN"
     gene            complement(22619..22843)
                     /locus_tag="ACHK74_003398"
     CDS             complement(22619..22843)
                     /locus_tag="ACHK74_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP0222156.1"
                     /translation="MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDA
                     FERYRKAALDAEFAEIMQSHGTAYTELSKR"
     gene            22909..23264
                     /locus_tag="ACHK74_003399"
                     /pseudo
     CDS             22909..23264
                     /locus_tag="ACHK74_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(23497..23607)
                     /locus_tag="ACHK74_003400"
                     /pseudo
     CDS             complement(23497..23607)
                     /locus_tag="ACHK74_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
BASE COUNT         6173 a         5616 c         6326 g         5493 t
ORIGIN      
        1 gccggtgtct cctgccatca acgataagaa gtcagattca ggtgtgaatg ttccggcagg
       61 ggggatcgag caggagctga aaatgaaacc ggaagaagag atggaacagc aactgccacc
      121 cgggatcagt gaatccggtg aagtggtgga tatgaccgct tatgaggcat ggcaacagga
      181 aaatcatccg gacatccagc agcagatgca gcgtcgtgaa gaggtgaaca ttaatgtgca
      241 ccgggagcgt ggggaggatg ttgagccggg agatgatttc tgatgatgag tatcgcgcag
      301 gtcagatcgg ccggaagtgc cggtaactat tataccgaca aggataatta ctatgtactg
      361 ggcagcatgg gagaacgctg ggccggcagg ggggctgaac agctgggact gcagggcagt
      421 gtcgataagg atgtttttac ccgtcttctg gagggaaggc tgccggacgg agcggatcta
      481 agccgcatgc aggatggcag taacaggcat cgtcccggct acgacctgac cttctccgcc
      541 cccaaaagtg tctccatgat ggccatgtta ggtggcgata agcgcctgat tgatgcacac
      601 aaccaggccg tggactttgc tgtccgtcag gtggaggcgc tggcctccac acgggtgatg
      661 acggacggac agtcagaaac ggtactgacc ggtaatctgg tgatggcact gtttaaccac
      721 gacaccagtc gcgatcagga accacagtta cacacgcatg cggtggtggc taatgtcacg
      781 cagcataacg gtgagtggaa gacactgagc agtgacaaag tggggaaaac ggggttcatt
      841 gagaatgtgt acgctaatca gattgccttt ggcaggctct accgggaaaa actgaaagag
      901 caggttgagg cgctgggcta tgaaaccgaa gtggtgggta agcacggtat gtgggagatg
      961 ccgggcgtac cggtggaggc cttttccgga cgctcacagg ctatccggga ggccgtcggg
     1021 gaggatgcct cgctgaaatc ccgggatgtg gcggcactgg atacgcgaaa atccaaacag
     1081 cacgtcgatc ctgaagtcag aatggctgaa tggatgctga cgctgaagga aaccgggttc
     1141 gacatccggg catatcgtga cgcggcggat cagcgtgcgg agacccgcac gcagacaccc
     1201 ggacccgctt cacaggacgg gccggatgtg cagcaggcgg tgacacaggc gattgccgga
     1261 ttaagtgaac gaaaagtgca gtttacgtac acggacgtgc tggccagaac ggtcggtata
     1321 ctgccgccgg aaaatggtgt gattgaacgg gcacgcgccg gtatcgatga ggccatcagt
     1381 cgtgagcagc ttatccccct cgaccgtgag aaggggctgt tcacatcagg aattcatgtg
     1441 ctcgatgaac tgtccgtccg ggcactcagt cgtgacatca tgaaacagaa ccgggtgacc
     1501 gtacatccgg agaaaagtgt ctcccggacg gctggttaca gcgatgcggt cagtgttctg
     1561 gcacaggacc gtccgtcgct ggccattgtg tccgggcagg gcggtgcagc cgggcagcgt
     1621 gagcgggtgg ctgaactggt catgatggcc cgggagcagg ggcgggaggt gcagattatc
     1681 gctgctgacc gtcgctcgca gatgaacctg aagcaggatg aacggttgtc cggtgagctg
     1741 ataaccggac gtcgtcagtt gctggaaggc atggccttca caccgggcag taccgttatc
     1801 gttgaccagg gcgaaaaaat ctccctgaaa gagacgttaa ccctgctgga cggtgccgca
     1861 cgtcataacg tacaggtcct gataaccgac agcgggcagc gaaccggtac aggcagtgca
     1921 ctgatggcca tgaaggatgc cggggtgaac acatatcgct ggcagggggg agaacagcga
     1981 ccggccacca tcatcagtga accggaccgt aatgtccgct atgcccggct ggccggagat
     2041 tttgcggcca gcgtgaaagc cggagaagag agcgtggcac aggtcagcgg ggtacgggaa
     2101 caggccatac tgacacaggc cattcgcagt gagctgaaaa cgcagggcgt gctcggacac
     2161 cctgaggtga ccatgactgc actgtcaccg gtctggctgg acagccggag ccgttatctg
     2221 cgggatatgt accgtccggg gatggtgatg gagcagtgga acccggagac acgcagtcat
     2281 gaccgctatg ttatcgaccg ggtgacagcg cagagtcaca gcctgaccct gcgggatgca
     2341 cagggcgaaa cgcaggtggt gcgtatttcc tccctggaca gcagctggtc gctgttccgg
     2401 ccggaaaaaa taccggtggc agacggtgag cgtctgaggg tgacagggaa aattcccgga
     2461 ctccgcgtct ccggcggtga ccgcctgcag gtggcatccg tcagtgaaga tgcgatgacg
     2521 gttgttgtgc cgggacgtgc tgaaccggcc accctgcctg tgagcgattc accgttcacg
     2581 gcactgaagc tggagaacgg ctgggtggaa acgcccgggc attccgtcag cgacagtgcg
     2641 aaggtttttg cctccgtcac acagatggcg atggacaacg ccaccctgaa cggtctggcc
     2701 cgcagcggtc gtgatgtccg gctgtattcc tcactggatg aaacccgtac tgcggaaaaa
     2761 cttgcccgcc atccctcctt tacggtggtt tctgagcaga taaaggcgcg tgccggtgag
     2821 acattgctgg aaaccgctat cagtctgcag aaagccgggc ttcacacgcc ggcacagcag
     2881 gccattcatc tggcccttcc tgtgctggaa agtaaaaacc tggccttcag catggtggac
     2941 ctgctgacag aggcgaagtc gtttgctgca gaaggaacca gttttactga actgggaggg
     3001 gaaatcaatg cgcagataaa acgcggtgat ttgctgtatg tggatgtggc aaaaggctat
     3061 ggcacaggcc tgctggtttc ccgtgcgtcg tatgaggcag aaaagagcat tcttcgccat
     3121 attctcgaag gtaaggaggc ggtcacgccg ctgatggaga gggtacccgg cgaactcatg
     3181 gagacactga cgtcggggca gcgtgccgcc acccgcatga tactggaaac gtccgaccgt
     3241 ttcacggtgg tgcagggtta tgccggtgtg ggtaagacca cacagttccg ggcggtgatg
     3301 tcagccgtga agatgctgcc ggagagtgag cgtccccgtg tcgtggggct gggccccacg
     3361 caccgtgcgg tcggtgagat gcgcagcgcc ggcgtggatg cacagacact ggcatccttt
     3421 ctgcatgaca cgcagctgca gcagcgcagc ggagaaacgc cggatttcag caacacgctg
     3481 ttcctgctcg atgagagctc tatggtgggc aataccgaca tggcacgggc atacgccctg
     3541 attgcggccg gtggtggtcg tgccgtggcc agcggtgaca cggaccagct gcaggctatc
     3601 gcgcccggtc agcctttccg tctccagcag acgcgcagtg ctgccgatgt ggtcatcatg
     3661 aaggagattg tgcgtcagac gccggaactg cgggaggcgg tatacagcct gattaaccgg
     3721 gatgtggaaa gggcactgtc cgggcttgag agtgtgaaac cgtctcaggt gccacgtcag
     3781 gagggcgcat gggcaccgga gcactccgtg acggagttca gtcacagcca ggaagcgaaa
     3841 ctggcagaag cgcagcagaa ggcgatgctg aaaggcgagg cttttccgga tgtccccatg
     3901 acactgtatg aagccattgt ccgcgactat accggcagaa caccggaagc acgggagcag
     3961 acgctgattg tcacgcacct gaatgaggac cggcgcgtac tgaacagcat gattcatgat
     4021 gtacgggaaa aggccggtga gctggggaaa gagcaggtca tggtgcctgt cctgaacaca
     4081 gcgaatatac gtgacggaga gctgcgtcgt ctctccacct gggagacaca tcgggacgca
     4141 cttgccctgg tggataatgt gtatcaccgg attgccggta tcagtaagga tgacgggctg
     4201 ataaccctgg aggatgcgga aggtaacacg cggctgattt cgccccggga ggcggtggct
     4261 gaaggtgtca cactgtacac cccggacacc atccgggtgg ggaccggtga ccggatgcgc
     4321 ttcacgaaga gtgaccggga gcgcggctat gtggccaaca gcgtctggac ggtgatggcg
     4381 gtttccggtg acagtgtcac gctttctgac ggacagcaga cccgggtgat tcgccccggc
     4441 caggagcagg cagagcaaca tattgacctg gcctatgcca tcaccgctca cggtgcgcag
     4501 ggggcgagtg aaacctttgc catcgcgctt gaaggcacgg aaggtaaccg gaaacagatg
     4561 gccggctttg agtcagccta tgtggccctg tcgcgtatga agcagcatgt gcaggtgtac
     4621 accgataacc gtcagggctg gacggatgcc atcagcaaag ccgtacagaa aggaacggcc
     4681 catgatgtgc tggagccggg ggctgaccgg gaggtcatga atgcagagcg gttgttcagt
     4741 acggcacggg agctgcggga tgtggcagct ggccgtgctg ttctccgcca ggcgggactg
     4801 gccgggggcg acagtccggc acggtttatt gcaccggggc gcaaatatcc gcagccgtat
     4861 gtggccctgc cggcttttga ccgtaacggc aagtccgccg gtatctggct gaacccactg
     4921 accacggatg acggaaacgg gctgcgggga ttcagtggtg aaggccgggt gaaaggcagc
     4981 ggggatgcgc agtttgtggc cctgcagggc agccgtaacg gcgagagcct gctggctgat
     5041 aatatgcagg atggtgtctg gattgcccgt gataaccccg acagcggtgt ggtggtgaga
     5101 atcgccggtg aaggtcgtcc gtggaatccc ggtgccatca ccggtggccg cgtgtggggg
     5161 gatatcccgg acaacagcgt ccagccgggg gccggaaacg gtgagccggt cacggcagag
     5221 gtgctggcac agcggcaggc tgaagaggcc atccgtcgtg aaacggaacg ccgtgcggat
     5281 gagattgtca gaaaaatggc agaggaaaaa cctgaccagc cggatggtaa aacagagcag
     5341 gctgtcaggg ctattgccgg gcaggagcag gacagggctt cgtcatcaga acgggaaact
     5401 gcgctgccgg aaagtgtgct gcgtgagtct caacgggtgc aggacgctgt ccgggaggtt
     5461 gcccgggaaa atctgctgca ggagcgactt cagcagatgg agcgggatat ggtccgtgac
     5521 ctgcagaaag agaaaaccct gggcggagac tgatacagga agacaaacgc tgatgacaac
     5581 ggataacacg aacacgacac gtaacgattc actggctgcc cggaccggta cctggctgca
     5641 gtcgttgctg gtctggtcac ccggacagcg ggatatcatc aaaacggtgg cactggtgct
     5701 gatggtgctg gaccatgcaa accgcattct gcatctggag cagacgtgga tgtttctggc
     5761 cgggcggggc gcgtttccgc tgtttgcgct ggtctgggga ctgaatctgt cccgtcatgc
     5821 gaatatccgg cagtcggcta ttaaccggct gtggggatgg gctgtgattg cccagttcgc
     5881 gtattacgtt gccggctttc cctggtatga ggggaatatc ctgtttgcct ttgcagtggc
     5941 ggcacaggtg ctgacgtggt gtgagacgcg cagcctgtgg cgtactgccg cggccatact
     6001 cctgatggca ctgtgggggc ctttgtccgg caccagttac ggcattgccg ggctgctgat
     6061 gctggcagtc agtcaccggc tgtaccgggc ggaagacaga gcggaacgtc tggcgctgct
     6121 tgcatgcctg ctggccgtta ttccggcact taaccttgcc tccagtgatg tggcggccgt
     6181 agctggtctt gtaatgacgg tgctgaccgt tggtctggtg tcgtgtacag ggaaatcatt
     6241 accccgtttc tggcccggag attttttccc ggtattctac gcctgtcatc ttactgtgct
     6301 gggcgttctg gctgtgtgac tggtatgatg ctggcgctga ttctgcttat taataaacaa
     6361 cataacacag gatatttatg aaaattacgg atcacaaact ccctgaaggt attgctctga
     6421 catttcgtgt cccggaagga aatataaaac atcctttgat tctcttatgc catggatttt
     6481 gcggtatccg gaatgttctg ctgccttcct ttgcgaatgc gtttacagaa gccgggtttg
     6541 caacgattac ttttgattat cgtggattcg gtgagagtga aggggaacga ggtcgtctgg
     6601 ttccggcaat gcagaccgaa gatattattt cagttatcaa ttgggcagaa aagcaggtat
     6661 gtattgataa tcagcgtata ggattatggg gaacatcgct gggtgggtgt catgtattca
     6721 atgcagctgc gcaggataaa agagtgaaaa gtattgtcag ccagctggct tttgctgacg
     6781 gtgaggttct ggttaccgga gaaatgaatg aactggagaa agcttctttt ctgtccacgc
     6841 tgaataaaat ggcagaaaag aagaaaaata ccgggaaaga gatgtttgtg ggggttaccc
     6901 gggtactgag tgataatgaa tcaaaggtat tcttcgagaa ggtaaaaggt cagtatcctg
     6961 aaatggatat aaaaatacct ttccttacgg ttatggagac gttgcaatat aaaccggcag
     7021 agagcgcagc caaagtacag tgcccagttt tggttgttat tgccggccag gatagtgtta
     7081 atccaccaga acagggaaga gccttatatg atgctgttgc ttctggtact aaggaattat
     7141 acgaggaagc tgacgcatgc cattatgata tttatgaagg cgcgtttttt gaacgtgtgg
     7201 cggcagtaca gacgcaatgg tttaaaaaac atctttaatt gtcggtatta atatgtcaga
     7261 ggtcgttgag gtcagcttaa tatcatagtg agtagtttca acgacctggg ggacatattt
     7321 tcacaaaggt cagggcaatc atgacagagc agaaacgacc ggtactgaca ctgaaacgga
     7381 aaacggaagg ggaaacaccg acccggagcc ggaaaaccat catcaatgtc accacgccac
     7441 caaaatggaa ggtgaaaaag cagaaactgg cggagaaggc tgcccgggaa gcagagctgg
     7501 cggcgaagaa agcacaggcc agacaggcgc tgtccattta tctgacgctg ccctcgctgg
     7561 atgaggcggt gaacactctg aaaccctggt ggccgggatt gtttgacggt aacacgcccc
     7621 ggcttcttgc ctgtggtatc cgggaggttt tactggatga ggtgtttcag cggaatatcc
     7681 cgctctctca taaaaaactc agccgggcgc tgaaagccat cacccgttcg gaaagttatc
     7741 tgtgtgcaat gaaagccggc gcctgccgct atgatacgga aggctatgtg acagaacata
     7801 tcacacagga agaagaacag tatgcgcagg cgcgcctgga aaaggtgcgc agacagaacc
     7861 ggatcaagga tgagctgcgg gcgatcctgg cagaataata aaaacgccct ctgtatgaca
     7921 gagggcaaca gcgaaaaaat cgggaaaaca gacatccaga gttttgatgg agaaaaacac
     7981 ccaaccgttc agggatgttt cataatgttt ctgacataat ccgggagccg ggtcaagagg
     8041 ggggataaaa aatggctgta tccagtggat tatctgagca cagcggtatt tatcgctatc
     8101 agcgcgtggg gcgtcatgcc gtccgtcagg agattcgtgg ttttttcagg gaagccgctg
     8161 aggcctggca gaatgcggcc cggctggcac cctgtgaagc atggcgggat tttgccctga
     8221 agagagcatc tgcatgccgg tgtaagtgcc gataaaaata acccccgttg cggagcgccg
     8281 ggggatcaga cttatatggc agatcgttat tattatagtg gtgtaagaag gagttgccct
     8341 gtctttcaaa cggacatcac agaattaaca tatttcacat cccgggtaag ccaacaggga
     8401 taaaaaatgt gatgaatttc aagaagcaga aggtgacagt ggcatgcata acagtacggt
     8461 tatgtccggt gaatctgaca gacagccggg ctgtattcgc ggcaggataa agaaaaaccg
     8521 ggagatgact ctcccggtcc gtcttatcgc taatcccctg agcagggcat gtatgactta
     8581 tctatgaccg ttatattcag cgccacatcc ggtagcctgc gatataacac agttatccta
     8641 tcagtaagag caggtatgat gaatacacgg agattaaaaa tataatctcc gtcacatgtt
     8701 acccccggaa accggtaacg ccgtcacggg aaatacgtga cagaaaaagc agcctccgtt
     8761 actccaggta tcagaaggct gaataatgca tgagactttc tttcttatgt gtctggtcag
     8821 gcggggtgac cagcgttttt agtaacacat agccatatgt gtattaagcc acctcatctt
     8881 attttgtcga attgatcaca attcaacggt ttatttttgg cgtgatgcgt gagccgttat
     8941 tcatcccggc atggaattca tgcaatcacg aaaagtaatc agtgacggag agtaaaatga
     9001 agaaatatat accagtggta ttatttgttt tttcatgtca ggtattttct gctgatattc
     9061 atggacgtgt tgtgcgtgtt ctggatggcg ataccattga cgtcatgtta tctcagcatc
     9121 cggtgcgtgt ccgtcttgtt aatattgatg cacctgaaaa gaaacaggac tatggccggt
     9181 ggtctgaaaa aataatgaaa tctctggttg cgggaaaaac ggttactgtc acatattttc
     9241 agcgagatcg ttatggtcgt attctcgggc aggtttatgc accagacggt atgaatgtta
     9301 atcagtttat ggtccgtgca ggcgctgcat gggtttatga gcagtacaac accgatcctg
     9361 ttcttcccgt cctgcaggaa gaagcacgac agcagaaacg tggtctgtgg gcagacagcg
     9421 ctcctgttcc gccctgggta tggcgctcac ggaaataatg atatttctgt ggctattgag
     9481 ttctcattga aatgcgatat ttaaaagcat gaagcatatt acattattaa cttatttctc
     9541 tgttaatgaa aggtgaacaa atgaactatc aggcagtatc agaattaatc acatcgtcta
     9601 atcataatgt tcttattgtg ggggactcag cttctgaagt tgatagcttt ttaaataaac
     9661 tgaatattac tgattataag tattatgatt tcagccagat ttacagttgt tctgacagaa
     9721 cattaaatga ttatgcggtt atttttatca gggatgcact gaacgccagt gagcatatta
     9781 ttatttttaa ttgtacagga tggcctgatt tgaataatga atctgccgtc atgcagtttg
     9841 ccagagtggc acgaaaatca ggaaaacaac tgatagtcgc agtaagagaa caggatatga
     9901 aaaaaatgga agcagagttt ggaaggatca taaaaatcca ctgacgcctt cgcgttgctc
     9961 agttgtccaa ccccggggac gggaaaaagc aagttttccc cgcccccggc gtttctgtag
    10021 tgaaaagcgg gaaatggagg gaatggtcag taacagggaa tgtggtatta tcgcggcggg
    10081 tgtctgagcc tttctggttc aggcaagacg caggtaccag aaatgcgaag accccacttg
    10141 ttaatccatt aactcgtgag gcctgcatga agtacttgaa cactactgat tgtagcctct
    10201 tccttgcaga gaggtcaaag tttatgacga aatatgccct tatcgggttg ctcgccgtgt
    10261 gcgccacggt gttgtgtttt tcactgatat tcagggaacg gttatgtgag ctgaatattc
    10321 acaggggaaa tacagtggtg caggtaactc tggcctacga agcacggaag taagctgccg
    10381 ggcggggacg gaagtccccg ctttccggaa gtgtgaggta tttcaggggc agacacccga
    10441 catgccagaa accgccggtc ccgcccgggg ccggcacaca ggttaaggca tttcctgttt
    10501 ttcagccatt tcgcaacaaa aatcacatta aacaacagta atcaccgttg atttgtgcgc
    10561 caacacagat cttcgtcaca attctcaagt cgctgatttc aaaaaactgt agtatcctct
    10621 gcgaaacgat ccctgtttga gaattgagga ggcgagatgt cgcagacaga aaatgcagtg
    10681 acttcctcat tgagtcaaaa gcggtttgtg cgcagaggta agcctatgac tgactctgag
    10741 aaacaaatgg ccgctgttgc aagaaaacgt cttacacaca aagagataaa agtttttgtc
    10801 aaaaatcctc tgaaagatct catggttgag tactgcgaga gagaggggat aacacaggct
    10861 cagttcgttg agaaaatcat caaagatgaa ctgcagagac tggacatact aaagtaaaga
    10921 ctttactttg tggcgtagca tgctagatta ctgatcgttt aaggaatttt atggcgggcc
    10981 acgccgtaag gtggcaggga actggttctg atgtggatgt acaggagcca gaaaagcaaa
    11041 aaccccgata atcttcttta actttggcga gtcagaaaga ttaccggggc catctaaaaa
    11101 ccgtatagcc aacaattcag ctatgcgggg agtatagtta tatgcccgga aaagttcaag
    11161 acttctttct gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt
    11221 caacaaacgt attaccgcca ggtaaagaac ccgaatccgg tgtttacacc ccgtaaaggt
    11281 gccggaacgc tgaagttctg cgaaaaactg atggaaaagg cggtgggctt cacctcccgt
    11341 tttgatttcg ccattcatgt ggcgcatgcc cgttcgcgtg gtctgcgtcg acgcatgcca
    11401 ccggtgctgc gtcgacgggc tattgatgcg ctgctgcagg ggctgtgttt ccattatgac
    11461 ccgctggcca accgcgtcca gtgttccatt accacactgg ccattgagtg cggactggcg
    11521 acagagtccg gtgcaggaaa actctccatc acccgggcca cccgggccct gacgttcctg
    11581 tcagagctgg gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt
    11641 ccgaccgata tcacgttcac accggcgctg tttgccgccc ttgatgtgtc tgaggatgca
    11701 gtggcagctg cgcgccgcag ccgtgtggta tgggaaaaca gacagcgcaa aaagcagggg
    11761 ctggataccc tgggtatgga tgagctgata gcgaaagcct ggcgttttgt ccgtgagcgt
    11821 ttccgcagtt accagacaga gcttaagtcc cacggcatga aacgtgcccg tgcgcgccgc
    11881 gatgcaggca gaacccgcca ggacattgtc accctggtga aacggcagct gacgcgtgaa
    11941 atcgcggaag ggcgtttccg gggaagcctg gaggccgtga agcgcgaaat cgaccgtcgt
    12001 gtgaaggaac gcatgataat gtcgcggaac aacaactaca cacggctggc cactgcctcc
    12061 ccctgagttt tacctcatct gaataatccg gcctgcgccg gaggcatctg cacgcctgaa
    12121 gcctgtcagc gaacaaaaaa acagcaccac atacaaaaaa caatctcatc atcccgcttc
    12181 tggtgcatcc ggcccccctg ttttcgatac aaaacatgcc tcactgacgg ggaattttgc
    12241 ttatccacat ttaactgcaa gggacttccc cataaagtta caaccgttca tgtcataaag
    12301 cgccagccgc cagccttaca gggtgcaatg tatcttttaa acacctgttt atatctcctt
    12361 taaaccactt aactacattc atttaaaaag aaaacctatt cactgcctgt cctatggaca
    12421 gacagatatg cacctcccac cgcaagcggc gggccccgac cggagccact ttagttacaa
    12481 cactcagata caaccaccag aaaaacccaa gtcccgcgca gaactgaaac cacaaagccc
    12541 ctcctcataa ctgaaaagcg gccccgcccc ggcccaaagg gccggaacag agtcgctttt
    12601 aattatgaat gctgtaacta atcatcttca tcgctgtcag tcttctggct ggaagtcctc
    12661 agtacacgct cgtaagcggc cctgacggcc cgctaacgcg gagatacgcc ccgacttcgg
    12721 gtaaaccctc gtcgggacca ctccgaccgc gcacataagc tctatcatag ctgacagcgg
    12781 gtatggcttt gcagggtggg gatgggtaag gtgaaatcta tcaatcagta ccggcttacg
    12841 ccgggcttcg gcggttttac tccggtatca catgtaacca cagcgtgccg ccttacatgc
    12901 gctgacgcgc ataaacaaca agttgctaca ttagcttaca ggttaattca ccttgcataa
    12961 gattatgcat ataggttaat gtttatcgaa atgggaggtc atatgtttag cgttgtttat
    13021 cacccggaag ccagggaaga agccacagca ttacccataa aaatcagggt gaagtttgac
    13081 cgtctcatca gcaaactgga atatgacgcc cgattactgc gggaaccgga tacaaagccc
    13141 ctgggggatg gactttttga gatccgcacg atggggacgg acatagccag aggtatctgg
    13201 gtataccaca aaggcaatac catcatcatg ctccgggttt tcatcaagaa gtctcagaaa
    13261 acgccagcaa aagaaatcga tctcgccaaa aagcggttag cggaggtact taatgaaact
    13321 ggtaagccat aaagaactgc ataacgagtg gatgcaggac gacgaatatc gcgctgcatg
    13381 gcaggaagaa gaacgtaagg agaaactcag ggagttgctt gcagaatggc gtaagcatga
    13441 caatttgaca aaagcgcagg ttgctgaacg catgggggtt actcctcctg ttatttcccg
    13501 cctggaaaac aacatcacta aagccagcat cgacaccctg actcgctatg cccatgcgtg
    13561 cggtatcaaa aaacctgtta ttacgctgta ctgaactctg ctctatggct gccagttctg
    13621 gagaaatcag taactaaatt ttaattagct acgttatggt aaatctgcct gatgtgtcga
    13681 tttctctgaa agtaattcgg cttctgtggg ctgcgtttgc cttgtgccac ctgaatgtaa
    13741 tcactatgat taaatatgat taacaactaa tcggatatac aaatgaaaaa caataccgca
    13801 caggcaacaa aagtaattac cgcgcatgtg ccattaccta tggctgataa agtcgaccag
    13861 atggccgcca gactggaacg ctcccggggc tgggttatca aacaggcact ttctgcatgg
    13921 cttgcccagg aggaggagcg taatcgcctg acgctggaag ccctggacga tgtgatatcc
    13981 ggacagatta tcgaccatca ggctgtacag gcctgggcag acagcctcag tactgacaat
    14041 ccgttaccgg ttccacgctg atggaactga agtggaccag taaggcgctt tctgatttgg
    14101 cgcggttata tgattttctg gtgctggcca gtaaacctgc cgccgccaga atggtacagt
    14161 ccctgataca ggcaccggtc attctgttaa ctcatccacg tatgggagaa cagttgtttc
    14221 agtttgaacc cagggaggtc agacggattt ttgctggcga gtacgaaatc cgttacgaac
    14281 ttaatggcca gactatttat gtattgcgcc tgtggcacac acgagaaaac aggtagattg
    14341 cgtgtgggga caacttaaca tagcctgcag atattaattc aggaacatta ttatcaggcc
    14401 agagattttg cattccatta aataatcttg acgtatagga aattgaaaaa ctgagcgata
    14461 cccctgtccg cgccttatgc cacgtgagct tttctgcttt tcccaccact ccggtgtttt
    14521 ctctctatgt atggtcggtt tttatacctt aatgggccgt tttttatact tttcccggat
    14581 tgcaggcgca cctctcctgc atcagccagg gatatacctc agcaggctta tatcccgcgc
    14641 gtttttcttc agccctgata cattcagccc cgcaatggcc cggcggtata ccatccggca
    14701 gccacacagc acgtattcga acgggtcacg actcaggaac tgcgcttcac catttgtgca
    14761 tagcacactt tcgccactgc ttccggttta tccatcccca gagcacggta cacctgcggc
    14821 agcttctctc cacacacccg gttggcaagg aacccgaagt acctcaccat cttaaaaaac
    14881 ttctccggga tgtgctgttt cagcctcgcg accagctcac gctgtgtcag cgtttccgtc
    14941 gccgtttctc ccgttttgtg gtccaggtaa cggaagctca ggctcgcccc tccgttgtaa
    15001 tgtgccagcc gggaggctgc tatcggcggc ttcttcagat aacgacccag gtggcgcgcc
    15061 gtattcctcc cgtcggccgt tttcttcgac atgtacatca ggcgcggaac tcatcggggc
    15121 gcgggaatga aatcccattc agactccgga tagattcaag caagcccact tatcgtcaaa
    15181 atcggtgttg caaaaacggg agtgaccata gatccgtttt ccaagcggac cccataatat
    15241 cggctggtaa acatcactcg ctagtgcatt ctttttgttg gtaatgataa ttatttacat
    15301 ttattctttg atgttttatc ataattaatg ttttgtcaat aaatatatgg aaacatattg
    15361 tttcagatgg gaatatgaga aagttcatcc ttatttctat gataacgtta gctagtgtta
    15421 gtctgggagc ctgcagacag aatgtcacca taaagcagga tgcgccaggg cagaaagctt
    15481 tcctgactga cgggcagatt tacgatttac acagtggcaa aattatttct agcagcgagc
    15541 ttttggcgga tcttgctacc gctcgacacc ttattattgg tgaaaaacac gataatgccg
    15601 agcatcatca gattgagctt tggcttattc aaaatctgct catccagcga ccacagggga
    15661 gtgtgttgct tgagatgttg acgtcagagc aacagccccg agttaaccaa gttaaatgtt
    15721 ggctgaaaga taatcctgtg gttcgagata gccgtgttca ggaactactt aactggcaaa
    15781 aagggtggtc ctgggagatg tatggcgaca tcgtcatgca gctattacgt gggccttacc
    15841 cgctgctcaa tgccaacatt ggccgggagc agatgttggc actgtacaaa aaaaacgaat
    15901 ttcctaaagg aaaaaaatcc actgcacctg tggtacaaga agctctgcgt gaaaccatca
    15961 tagctatgca tgaaggcaac cttgaaagcc agcaactgac gtcaatgctc tctgttcagc
    16021 agcagcggga tcgctacatg gcccgccagc ttctgagtgc cccggtaccg tcgttgttga
    16081 ttgcaggtgg ttatcacgcg tcaaaaagca tgggcgtacc gttacacatg gaagatcttg
    16141 ccgccggcac ccatccagtg gtgttgatgc ttgcagaaaa aggaatgaac atcacagtag
    16201 atcatgctga ctacgtgtgg tttgtagcgc cagatacaac aaaaaggtaa accatggatg
    16261 atgaatattc tccacttccc acagtcggta aaatggagca gctggtttat ctgttctcta
    16321 ctgcttcatg gactgatttt ccttgcgttt atctggcgtt tttccgaagt tcagcccgct
    16381 atgtctcctg caccggcgat catgctgcaa tgggcagaaa ttatagaggc tccttcttct
    16441 ccactttcgc ttccggtggg aatcgctcaa caggagtccg cagtgacaga agaaaagcaa
    16501 caaactgagg acagacaaca acgtcctgtc actgaagaca gttatgcaac cattgaaata
    16561 accaggaaga aaaagtcatc cgatggagaa aagagaaaga cacgtccacc tcgtaagata
    16621 aaggctcaaa ccagcgacag caaccccaca gccgtttcca gtaatgctgc acctcaggct
    16681 ctggtcgaat cgtcccgtat tgctgcgcct tttaacagtg actcaacaaa gcgagataat
    16741 tctgaggcat cttgggaaag cagagtaaaa ggccacctta accgttataa acgttaccct
    16801 ggcgatgcca gaaaacgtgc acgaaccggc acagccgtcg tgagttttac cgtaaacacg
    16861 gaaggcacta ttgtcagtag tttcttggaa atctcttcag gaaccctttc tcttgaccgc
    16921 gaagctattg ctgttttaga acgcgctcaa ccacttccca aaccaccgcc tgaaatattg
    16981 gaggggggat tatttaaagt aaaaatgccg attaccttta agttgaagga gtaaagggat
    17041 aattggccgt tcaagttcat ttaactgaaa tttaattttc atttttatgc tgaacatttt
    17101 atcaaacttt tccactgtcg aaaaaatcaa aaagacagta gatcataatt aataaacaaa
    17161 tagttatact tatatttatt aatattatat ctaaggaaat ttatgaacgt tataaaactg
    17221 gctatcggct caggcatatt attgctcagc tgcggtgctt actcacaatc catcagtgaa
    17281 aaaactaatt ccgacaaaaa aggagcggca gaattcagtc cgctcagcgt ttctgtcggg
    17341 aagacgacca gtgagcagga agctctcgag aaaacaggcg cgaccagttc ccggacaacg
    17401 gacaaaaacc tgcaatcact tgacgcaaca gtgcgtagta tgcccggtac ttatactcaa
    17461 atagatcctg gtcagggagc aatcagtgtg aatattcgag gcatgagcgg atttggtcgt
    17521 gtaaacacta tggtcgatgg tattacccag agtttttacg gaacctctac ctccggaaca
    17581 acgacgcatg gttcaactaa caatatggct ggcgtactta tagatcctaa cttactggta
    17641 gcagttgatg ttacacgcgg tgacagcagt ggctctgaag ggatcaacgc ccttgccggt
    17701 agtgtaaata tgcgtactat tggcgttgac gatgtaatat ttaacggtaa tacatatggc
    17761 cttcgttcac gtttctctgt cggtagtaat gggctgggac gcagcggaat gatcgccctt
    17821 ggtggaaaaa gcgacgcttt tacggatacg ggaagcattg gcgttatggc tgctgtgagc
    17881 ggcagttctg tgtactctaa tttctcaaat ggttctggaa ttaacagcaa agagtttggt
    17941 tatgataaat atatgaagca gaaccccaaa tcccaactgt ataaaatgga tatcagacca
    18001 gacgaattta acagcttcga actttccgct cgaacctatg aaaataaatt tacacgtcgt
    18061 gatataacca gtgacgacta ttacattaaa tatcattaca cccctttttc tgaattaatt
    18121 gactttaacg taacggccag taccagtcgc ggtaatcaaa agtatcgtga tggctcgctg
    18181 tatactttct acaaaacctc agcgcaaaat cgttctgacg cgctggatat caacaatacc
    18241 agccggttca ctgtcgcgga caatgacctg gagtttatgc tgggcagcaa actgatgcgt
    18301 acccgctatg accggaccat tcactcagcg gcgggcgacc cgaaagcgaa tcaggaatcg
    18361 atcgagaaca atccgttcgc accctccggc cagcaggata tttcagcgct gtataccggg
    18421 ctgaaggtta cgcgcggcat ctgggaggca gatttcaatc tcaactacac acgtaacagg
    18481 atcacagggc acaagcccgc ctgcgattca cgcgttatct gcgtgccaca gggtagctac
    18541 gatattgacg ataaagtggg tggcttcaac ccttcagttc agctttctgc tcaggtaaca
    18601 ccatggcttc agccgttcat tggctacagc aaatccatgc gcgccccgaa catccaggag
    18661 atgttcttct ctaattcagg aggcgcatcc atgaacccat tcctgaagcc tgaacgtgca
    18721 gaaacctggc aggcgggttt taacattgat accagagatt tagtggtcga acaggatgcc
    18781 ctgcgcttta aggctctggc gtaccgcagc aggatccaga actacatcta cagcgagtct
    18841 tatctggttt gttctggagg tcgtaaatgc agtatggctg aggtgattgg caatgactgg
    18901 gagggcatta gcgatgaata cagcgacaat atgtacatct acgttaactc ggcaagcgac
    18961 gttatcgcaa agggcttcga actggagatg gattatgatg caggttttgc ttttggccga
    19021 ctctctttca gccagcagca aacagaccag ccaacctcca tcgccagcac ctactttggc
    19081 gcaggggata tgaccgaact gcccagaaaa tacatgacgc tggatactgg tgttcgcttc
    19141 ttcgataacg cgttgaccct gggcactatc ataaaataca caggcaaggc tcgtcgcctg
    19201 tcgcctgatt ttgagcagga cgaacatacc ggcgcaataa tcaaacagga tttgccgcag
    19261 atcccaacga ttatcgatct ctatggtact tacgagtaca accgcaacct gacactgaaa
    19321 ctttcggtac aaaacctgat gaacagagat tattcggagg cgctgaataa gctcaacatg
    19381 atgccaggtc ttggtgacga gacccaccca gccaattccg cgcgtggcag aacatggata
    19441 tttggcgggg acattcgttt ctgatgacaa tttccgctag gttcagacaa tacgttttct
    19501 ctcttatgtc aattttattg caggaacgaa aaatgaatat tttcacttta tccaaaacac
    19561 cgctatacct gttaatttca ctatttttac ccacgatggc catggctatc gatccacctg
    19621 aacgcgaact ttcgcgattt gccctgaaaa cgaattacct tcagtcccct gatgaaggcg
    19681 tctatgaact ggcgtttgat aatgccagta aaaaggtgtt tgcagcagtc accgatcgtg
    19741 taaatcgtga agccaataaa ggctatctgt attcgtttaa ttcagattcg ctgaaagtcg
    19801 aaaataaata cacgatgcca taccgggcat tttcgctggc gataaatcag gataaacatc
    19861 agctctatat cggacacacc cagtcagcgt ccctgcgtat cagtatgttt gacaccccaa
    19921 ccggcaaact ggtaagaacc agcgacaggt taagttttaa agcggcaaac gctgcagatt
    19981 cgcgttttga gcatttgcgc catatggttt acagccagga ttccgatacc ctgtttgtga
    20041 gttatagcaa tatgctgaaa acggccgagg gcatgaagcc tctgcataag ctgttaatgc
    20101 tcgacgggac gacgcttgcc ttaaaaggcg aggttaagga tgcttacaaa ggtacagcgt
    20161 atggtctgac gatggatgaa aaaacacaga aaatctacgt tggcggaaga gattacatca
    20221 acgaaattga tgcgaaaaat cagacgctgc tgcgtaccat cccgttgaaa gatccgagac
    20281 cacaaatcac aagtgtgcag aatctggcgg tggactccgc ttctgaccgt gcctttgtgg
    20341 tggtattcga ccatgacgat cgttccggta caaaagatgg actctatatt tttgacttac
    20401 gcgacggtaa acagcttggc tatgtgcaca caggagccgg agctaacgcg gtgaaataca
    20461 atccgaaata taacgaactg tatgtcacca acttcactag cggcaccatc agcgtagtgg
    20521 atgccaccaa atacagcatc acccgtgaat ttaacatgcc ggtctaccca aaccagatgg
    20581 tgttgtcggg tgatatggat accctttaca ttggcatcaa agaaggcttt aaccgcgatt
    20641 gggatcctga tgtgtttgtg gaaggagcta aagaacgtat tctgagcatt gatttgaaaa
    20701 agtcgtgatc gggggaaaac atgggataaa aactccgtta ttccactaaa ctggccggac
    20761 taggaaaagc aaacatctct gtcctgccgg tacgcctgtt acgccgctcc atgaggctga
    20821 ggaatctcca gaaaacgaga caggcacagc attgtacgat caactgattt gtgccaataa
    20881 ttcaatgaaa tcacagctat gcctgttcat ggggttctag gccggaaccc cagatttttc
    20941 cgattgtttg ttttctaact gattattcag tagttttaga gggctgacgc tcagtggaac
    21001 gaaaactcac gttaagaggt tttgactatg ataaaagtca aagtaggggt gagtttggat
    21061 atggaaatta ttgtactata agggcttccc cgaacagtca acgtcggtat ttatggtttg
    21121 tctgcactac agttaaaaat aaaggggaga gactgcggta ctataaacgg tcataataaa
    21181 tcgttatcga ttcggaccct gatgaaagac gcgcgtgagg ctcttgttcg ttaagcggat
    21241 acgtatatcc tgtacaataa tcagatatac ctcacggggt ctaacccttt atcatcaacg
    21301 agttgcaggt cagatagtca tacggttgct gcgtgcttta gctggctgaa tacagtctga
    21361 aatcatcacc gtaatgcaat gatgcccagc acagccttgc atttttggcg gcgatggcca
    21421 caacagcgcg ccagtatcct ctgcgttcga ccagtgaaca gacccaacga ctgaatgagt
    21481 cagtcctttt ctcagcgcca atcagtactg aacgagcacc ctgaaccaga agcgttcgca
    21541 gatacgaatc accggctttt gttatcctgc ctagcttcga ctttccgccg ctgctgtatt
    21601 gtgatggagt cagtcccagc caggccgcca gttgccgtcc atttttaaaa tcatgtgcat
    21661 taccgataca ggcaaccagc gcacaggcag ttgtgggacc aattcctttt agctccatca
    21721 gccgctgact gcggtgatct gttctggcca tacgggacaa aattttgtca tattcagtga
    21781 tgttagcttc aatgcggggt cagtttggag atcgtaaaat tttgttcgtt aagcacatta
    21841 tttaaccaat agatatgcgt aacggattga ttttgcgtca acggctatcg cttttaagtt
    21901 atcgccattg aggtattgca tttaggttat ccgatagagg tcaaatcctc tgaaaacgcc
    21961 gtatagcacg ggagtacacc ctaccgcaat agcgcaaccg tacaatattg tacgtttccc
    22021 aaacgcagcc taggaagcac gtcgtcagta ttggagaaaa cttacaaacg ctcgccgcag
    22081 ccccagcgac cgaacgttag tgagcgagtg ggcgaggaag cggaaaagtg tcaggtctga
    22141 attgagcaca tacgccagaa attatatagc aaaaagatat atgattttta cattgctcag
    22201 tagattgtaa aatgctgctg attggatatc tatatctaat ttaaaataat ggctttaaga
    22261 tggtcagaaa ccatcttcca gttatattct cctgttgcag cattcaaagc gataatttgc
    22321 atttcatcaa acgaaatacg cgaactaata atatgtatac cattccgttt taaaaaaacc
    22381 tgtgcaacaa acaaggctgt tcgcttattt gcatcgttga aaatgtgacc acgagcaata
    22441 gcaactaaat acacggcagc cagatccata atatctgtca caccttcata atgatacatg
    22501 ttcaaaacac gatgaatgat cgcgtcagcg cgaccgggtt ctggcatacc cgccacgcca
    22561 ccatcacgcg aaatcaagcg gtcatgaaac tcaatcattt cctgtgaact aacgaatttc
    22621 atcgcttaga aagctccgta taggccgtcc catgactttg catgatctca gcaaactcag
    22681 catcaagagc cgctttacgg tatcgttcaa aagcgtcttt atcaaccagt acagccgcgc
    22741 cacctttacg gcgctgaatc tcaacaggct gattttcatc aaccgccagc tcgagcaacg
    22801 aggccaggtt atcgcgagca tggctatagt tacatgtaaa catagtgctt cctctttgag
    22861 ttgtacaatt aaattgtaca acagttatct tcaatatgaa atcaccgtta ccgctggcgc
    22921 atcctgaccc aacttaaccg gggagaaagt cgccatgccg ttgccagagc tatctgtcac
    22981 ggtcagaaag gtgagataag aaagcgatat accgatggtc aagaagatca actgggcgca
    23041 ctggggctgg tgactaacgc cgtcgtatta tggaacacta tttatatgca ggctgccctg
    23101 gaccatttca gggcacaggg ggaaatcgtg aatgatgaag atatcgcacg cctctcacca
    23161 ctttgccacg gacacatcaa tatgctcggc catattcctt cacgctggca gaactggtga
    23221 ctaaagggca aaaagaggca tcggaggaag aaaacattgc ttaacgtgag ttttcgttcc
    23281 actgagcgcc agccccctaa ttctctatat ccaaactgac cccaataggt gacgggttat
    23341 gtgacgaaat ctgatgtaga aatcgttgtt tcagtgacag tcactcaatc ggtcatttat
    23401 gtgacacccc acgccgttac tggtcgcgga aaaatccagc ggaaaaatcc aggtttccgg
    23461 cttgggaccc catctcaaga gatcctttga tcttttctac tgaaccgcga tccccgtcag
    23521 ttcagaagac gaagatgacg caacggttcc tcattgtaca tcacgaatat agtttgcttg
    23581 acatcctgac aagaataata ttttaatt
//
LOCUS       DAZGSP010000077        23035 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZGSP010000077 DAZGSP010000000
VERSION     DAZGSP010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.077"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(375..1376)
                     /locus_tag="ACHK74_003401"
     CDS             complement(375..1376)
                     /locus_tag="ACHK74_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP0222157.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCALAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1484..1780
                     /locus_tag="ACHK74_003402"
     CDS             1484..1780
                     /locus_tag="ACHK74_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP0222158.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1903..2328)
                     /gene="arsC"
                     /locus_tag="ACHK74_003403"
     CDS             complement(1903..2328)
                     /gene="arsC"
                     /locus_tag="ACHK74_003403"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFP0222159.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(2341..3630)
                     /gene="arsB"
                     /locus_tag="ACHK74_003404"
     CDS             complement(2341..3630)
                     /gene="arsB"
                     /locus_tag="ACHK74_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFP0222160.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3684..4037)
                     /gene="arsR"
                     /locus_tag="ACHK74_003405"
     CDS             complement(3684..4037)
                     /gene="arsR"
                     /locus_tag="ACHK74_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFP0222161.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            complement(4576..4749)
                     /locus_tag="ACHK74_003406"
     CDS             complement(4576..4749)
                     /locus_tag="ACHK74_003406"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222162.1"
                     /translation="MHPLTHPLPVTAHALLLDNGILTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            4777..4860
                     /gene="dinQ"
                     /locus_tag="ACHK74_003407"
     CDS             4777..4860
                     /gene="dinQ"
                     /locus_tag="ACHK74_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFP0222163.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4914..6266)
                     /gene="gorA"
                     /locus_tag="ACHK74_003408"
     CDS             complement(4914..6266)
                     /gene="gorA"
                     /locus_tag="ACHK74_003408"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFP0222164.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6338..7180)
                     /gene="rlmJ"
                     /locus_tag="ACHK74_003409"
     CDS             complement(6338..7180)
                     /gene="rlmJ"
                     /locus_tag="ACHK74_003409"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFP0222165.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            7383..9425
                     /gene="prlC"
                     /locus_tag="ACHK74_003410"
     CDS             7383..9425
                     /gene="prlC"
                     /locus_tag="ACHK74_003410"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFP0222166.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            9433..10185
                     /gene="rsmJ"
                     /locus_tag="ACHK74_003411"
     CDS             9433..10185
                     /gene="rsmJ"
                     /locus_tag="ACHK74_003411"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFP0222167.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(10234..11703)
                     /gene="dtpB"
                     /locus_tag="ACHK74_003412"
     CDS             complement(10234..11703)
                     /gene="dtpB"
                     /locus_tag="ACHK74_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFP0222168.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            11869..11922
                     /locus_tag="ACHK74_003413"
     CDS             11869..11922
                     /locus_tag="ACHK74_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222169.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(12021..12455)
                     /gene="uspA"
                     /locus_tag="ACHK74_003414"
     CDS             complement(12021..12455)
                     /gene="uspA"
                     /locus_tag="ACHK74_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFP0222170.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12846..13181
                     /gene="uspB"
                     /locus_tag="ACHK74_003415"
     CDS             12846..13181
                     /gene="uspB"
                     /locus_tag="ACHK74_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFP0222171.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(13252..14751)
                     /gene="pitA"
                     /locus_tag="ACHK74_003416"
     CDS             complement(13252..14751)
                     /gene="pitA"
                     /locus_tag="ACHK74_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFP0222172.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14983..16185
                     /gene="yhiN"
                     /locus_tag="ACHK74_003417"
     CDS             14983..16185
                     /gene="yhiN"
                     /locus_tag="ACHK74_003417"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP0222173.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAVQKQ"
     gene            16328..17170
                     /locus_tag="ACHK74_003418"
     CDS             16328..17170
                     /locus_tag="ACHK74_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFP0222174.1"
                     /translation="MARGRRLKSYQDYENALGDGIGLGYGQSYQPWLRAQDVRSHGNR
                     SIVFGLKTFRNHHFFSSVESNFFYLAEFNDLVIDIREQFPLIPLLLTHQIANHLDVQH
                     PLVRGVRGVPADVLNVMTTDFLLTLRMPGGGLRYKAIAVKQNESIPKREAEKLEIERM
                     FWQLIDVEFQIYTGSELNHVTGQNICWATSVLRNGSEFFDKYPLDKILWRLKPDVYPI
                     VELRAMISSIFDVDEQEAIMLLQAIIGLKMINVDLSYPILETGLIKIISNGYCIGLNA
                     NGYY"
     gene            17157..19283
                     /locus_tag="ACHK74_003419"
     CDS             17157..19283
                     /locus_tag="ACHK74_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0222175.1"
                     /translation="MDIIRNSVWQSHDSDVLSDGLYRVLDFDRDTDLLILFEIKKERT
                     SKPIPFSLSQFTYGVDSQRITHKKYTPPSYMLIDERKINNEDRKRRDVNYKIIKELVE
                     DKIFVFDYAIHKKSHLLTEYSRKKQIPQRTIRTLLTLYWKHGQDIYALLPAYPNCGAA
                     GKSRIRHEVKLGNAKKNRVLPNIRSKVYILNEGDIENIKKSLTRFHYKTHGETIKKTL
                     VRHIELYFKDEIKKANAENRAPYIPSLKQFTYWNRKLFSKEFSINKKNSKKEIALKMR
                     GLLGSAANTTVLPGDVFEIDSTVADVHLVSSLNRRKVIGRPTIYTVVDRASRMIVGLH
                     VSLYHASWRAARQALANCFMPKKEYCRIFGVSINEDEWPCSHIPLTLMCDNGEMIGLK
                     PQDKMTPLTKLEFAPVGRGDRKSIVERCFGILNDEAIHELTGATRRGKIVKGEPTPQS
                     RACLTIQEVTSVLIREILAHNQRTYEELAYINPLLIEHDLIISPKNSWMISVKQGRFS
                     GRVVTADEVIARLLIPVTANITAGGIQYNNLFYECDPDIASAARVFGRTSCEARIDDN
                     CVDYLYVRFDKNSVFRKHYLLKKRDVFKGKAHLDTDVMADWVDTQKEINAFTLNVFNN
                     SKVKDKINRQGIERLNDISDPRRVHGKDIRRNRKAELDLLGRTNLPNEKNEPVLPSSS
                     NILLLPGREEKQRWLNAKKKQEDAEK"
     gene            19280..20725
                     /locus_tag="ACHK74_003420"
     CDS             19280..20725
                     /locus_tag="ACHK74_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP0222176.1"
                     /translation="MNMLRSVSAIYKEPGLPEYRGNPLIEALPEALSDDEVFFEMSYF
                     PEIDEDIRWTAPAKIREQYVDRIKKFRCPQTNLIEAYRMIYRALRESYAARNPLKSGT
                     VQYLHYYGNERPDIEPDSGYFKSQAETITIVGMSGSGKTTMIEQIMDYFPQVIEHSSY
                     KGAFPGLSKQIVWLKINCPYNSSVRDLCEEILLKLDDASGIERTTPEIRNGPLARQIA
                     QRIKSSFLGILVIDEMQRLKFSRTGGENKLIDFLHEIVDSMGVSMVFCGNPPFDETLA
                     RKMRIARRAESGGYMNILNVRYDSQDWQSFIHYLWPLQWTNIETPLSDELNEKLFVLS
                     KGNIGLAQMIYRRAQLKVIGSGNELISSVVLSSSVPVLASHTEEYQEALILGAVGGYD
                     DKYCSVSHAGEASTDFTADRHAKALIPGDIDRPQHKEFAQKIDEAMQNFESKIISFDR
                     ERIISRAADNNETYSSLQRCGLLNTDPLNEL"
     gene            20768..22321
                     /locus_tag="ACHK74_003421"
     CDS             20768..22321
                     /locus_tag="ACHK74_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFP0222177.1"
                     /translation="MILLPVLPDETLFSRICRSVTVYGMSPSTMLQILFGKSDVSVHP
                     ILSSGLNDISLHTSESAEQLLYEQTLLPLFSWALPSSRKEIMDLRTTPGRLNRLCRLS
                     NFSSGLQTVLKFCPVCAREDAYHHGVTYWHLLHQLRGITTCYRHQILLESICVPPSPH
                     IRKGLMPPVSYIEHLSTEFEFDFAKFCYEFINMIRRGDANHPDYMDILKQLNLLSVSG
                     NIKKGVFYSHVYAKCRLLDSGSSAILPESISDYHYWEPLLRNQSCQHPTKHLLLCYCL
                     LRITWPVHADIHKKSKSIAFDDDTAQFCLLSEKHNSINSLAKELNKSRCFIKGVIHKK
                     YVKNYKCSIKINILTEFLIKSMAVRGFSLASIAKKNSLSEGSVSSVISSFYGLCSWRK
                     KCKRDSLRRRHKQKILRFMHGLTGPVTRKVVRANCYASFFWLNRFESQWLDFYLPKES
                     RRCNVKRVNWDERDKFSLSLIKNFLAQGQCSLSLSRLDSVLGGHGWLLKNRDKLPVTM
                     ALINMSKSK"
     gene            22337..>23035
                     /locus_tag="ACHK74_003422"
     CDS             22337..>23035
                     /locus_tag="ACHK74_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222178.1"
                     /translation="MIYENELWHSFLLRTQIIYNIQNYRNIISAHGALRYDAYPRPEL
                     INVYKLHRTQDLYDILTTVNGFILTSRMIYSPYGVYSYFDAMEDAHNKGFNIINTYPV
                     VNISRIRYCHSCILEDIRIKGVGYLRHRWQFESRCCHHNQSLYEVCFDNYSGAVKGLC
                     ELMVSGLNPSGCYSLVVDVFNPFQTPVYLLPCSDGRVRTWILENKDELVNFLCSIFKN
                     KSSSGLKRVIDVKIM"
BASE COUNT         6258 a         5301 c         5704 g         5772 t
ORIGIN      
        1 tatcaaaaag cgctcatttt aaaaagttat tatgttctcc gatagtgtgt gaagtaatat
       61 ttttgtgatg ttttagatgt gtaataatga accaggttaa agtgtattta aaaatgtgtt
      121 gtattagcga ctgaaatctg ccttttaaaa aggaggaggg tactgagaca aagagtcatt
      181 gcttctccga taaaatgtca gagaagcatg attacgtggt tattgtctgt tgatgacttg
      241 ttgaccgagc cgtcatcatt ctcaatgcat tagttaaatc atttgtgatt tttaaggatt
      301 atcctgtcta tgattttcta cttagaagga tatggcacta actatgcatc ttatagttta
      361 ctgctggcat ctatttaaaa tagcaattga gtcaaatagc ccatcaagat agccatacct
      421 aatataaccg tcaggaatgc agctatcatc ggcattctga acattgattt aagtaatatc
      481 acttccgtca ggcttgcgcc ggcactgccg atgattaatg ccattaatgc tcccagaccc
      541 attccttttg tcatcagaac agatgccaga gggataactg cctcagcccg gatatacagc
      601 ggaataccga caacggcgct taatgggatg gcgaagggat tatctgctcc tgcatgagcg
      661 gcaatccacg ctgagggaat aaagccataa ataaaagatc ctattaatac tcctaacaaa
      721 agatatggca gtacatcttt aaactgttgc aacgcatctt tggtggctag ttttatggga
      781 ttaatactta ctacagcttt ggtactgcaa caggtaacta cggggttctc aatggcttta
      841 gccgctggac tacaacaact cacttttatg ggcgtatatg tcgttcccgg cgaagttttt
      901 gctagagcac aacaatttgc tgatgagctt ttactggcaa tgatatgacg ttcaaacccc
      961 agagaatcca ggataatact ggcaagaacg gagacgccgg cggcgataat cgcgtacaac
     1021 aaggtgactt tccaaccaaa ggtcatccac attagtccga cgataatggg attaagtaat
     1081 ggtgaaacaa ataaaaaagt gagagtcgga ccaaacccgg ctttcgctga caacaatcca
     1141 cgtaacatgg gtattgtcga gcaactacag aacggggtaa cggctcccaa cagagcagcc
     1201 aggagatagc ctctcccttt tctggccccc atcatctgct ggattttatg gtctggcacc
     1261 ttttgtctta tcaggctgac accggcacta atcactataa acagcaacga aagctcgacg
     1321 gcgagaaaca caaacatctc tgcggcatct tgcagcatcg caagccaact actcatacgt
     1381 aacctcattg gataaatcta tatttccaga ataatcgaaa tgaaattcag atcaagatat
     1441 ttctggtatt atcgaaatat caaccagaca aagggggtgt catatgcagt tggaagaagt
     1501 tgctaaagcg ctgaaagagc taggtcaccc gacccgtttg ttcatattca aacacctggt
     1561 caaagcgggg gagcaaggtt tgccggtcgg tgagttgcaa aagcagcttg ggatacccgg
     1621 ctctacactc agtcaccata tttcagcact ggtatcggta gggctggtta aacaaaaccg
     1681 tgagagtcgg acgctgatgt gtgtttcgca atacgcaatg ctggaagcga taattgaatt
     1741 cttacgcgag gagtgctgcg taaatagcaa aattgcctga tgcgctacgc gctacagatt
     1801 tcatgccatg cacggttttg taggtcggat aagacgcgtt agcgtcgcat ccgacataaa
     1861 caaagcgcac tttaccaact atgggcagtg gatatcgccg ctttatttca accgcttacc
     1921 cgcttcatca accactttct cgccatcttc cttggagaat gcgccttttt gcgcatctgg
     1981 cagaatttcc agcaccactt ctgaagggcg gcacaggcga gttcccagcg gcgtcaccac
     2041 aatcgggcga ttaatcagaa tcgggtgctg aagcataaag tcgattaacc gatcgtcagt
     2101 aaatttatct tctgcaaggc ccagttcctc atacggctcg acgtttttac gcaacagcgc
     2161 gcgtacggaa atccccatat cggcaatgag tttgaccagc tcatcgcgcg ttggcggtgt
     2221 ttccagataa tagataatgg tcggttctgt accgctgttg cggatcatct ccagcgtatt
     2281 acgcgacgta ccgcaggccg ggttgtgata aatggtaatg ttgctcatat caatatctca
     2341 ttacaaagtg aaagagagac gtagcgccag cgcagccagc gtcacaaaaa gcaccggcag
     2401 ggtcatgata atccctgtac ggaagtaata tccccagctg atagtcatat tcttctgcga
     2461 aagtacgtgc agccagagta gcgtagccag gctaccaatt ggggtaattt tcggtcccaa
     2521 atcgcagcca atcacattgg cataaaccat cgcttctttg ataacgccag atgccgtgct
     2581 gccatcaatg gacaacgcgc caaccagtac cgtcggcata ttgttcataa tagaagagag
     2641 gaaggcggtg aggaatccgg tgccgagcgt cgcggcccac aggccgttat ccgccagcac
     2701 gttgagtacg ccagaaagat attccgttaa tccggcattg cgcaggccat aaaccaccag
     2761 atacatgccg agcgagaaga tgacaatctg ccagggggca ccgcgcagga ctttacccgt
     2821 attaatcgca tgaccgcgtt tagcgacgac aaataatatc agcgcgccca cagctgcaat
     2881 ggcgctcacc ggaatgccga gcggttccag gacgaaaaat cccaccagca gaagcagtaa
     2941 aacaacccag ccagttttga acgtagcagg atctttgatc gcttctgcgg gagatttcag
     3001 cagcgccata tcgtagttct gcggaatatc tttgcgaaaa tagagatgta acatcaccag
     3061 cgtggcaaca atcgcggcga tatccaccgg caccatcacc gaggcgtatt cgcgaaagcc
     3121 gaggccaaag aaatcagcgg aaacgatatt caccaggttg gagacaataa gcggcaggct
     3181 ggcggtatcg gcaatgaatc cggccgccat cacgaacgcc agcgtagtgc ctttactgaa
     3241 ccctaaagcc agcagcatgg cgatgacaat cggtgtcaaa ataagcgccg cgccatcatt
     3301 ggcaaacagg gcggcaacgg cagcaccgag caggacaatc caggtaaaca gcaagcgacc
     3361 acgaccatta ccccagcgtg agacgtgcag cgccgcccat tcaaaaaagc cggactcatc
     3421 cagcagcagg ctgatgataa tgacggcgat aaacgcagcc gtcgcgttcc agacgatatt
     3481 ccacaccacc ggaatatcac ccggatggac cacgcccgta actaacgcca gtactgcgcc
     3541 gagcgttgca ctccagccga tgcctaaacc tttcggctgc cagataacca atacgatggt
     3601 caggacaaag atagcgcctg ccagtaacat aatgcctccc ggataaaaca catctgaaaa
     3661 ttcatatgtg tttagctaaa tttttaactg caaatgttct tactgtcccc ggaacagttt
     3721 tgtcgagcca ggttgcggac aatcgcctga accttttcct gttcacatcg ccaggcctca
     3781 tcaataattt tcgccgccca tgctggaata tgcggtgata agcggtaatg aacccactta
     3841 ccttgcttgc ggtccagcaa tagcccgctt tcacgcagca atgccaggtg gcgggagatc
     3901 ttgggctgcg actggtcgag agcagtgcag agatcgcaga cgcataactc tcccagttcg
     3961 ctgagcagta aaacgatgcc cagacgggtt tcatcagcaa gaattttgaa caattggatg
     4021 ggtaacagaa atgacatatt gcgctcctga ttgttgcagg tagtgtctct cttcgaagcg
     4081 gataagtcaa aaacatatat gacttaacga atgtgtaagt gcagaggaag gtaataggtg
     4141 tgaattttga gttggctatt catttgaaag gaggtctgaa tcaggacgta aaaaaaccga
     4201 ctttgcgtcg gttttttact ttccagccct gagttggtgg ctctggctgg agtgagagag
     4261 ttcttatcta acagcccaat actattagcc agtggctaac attgcaagaa ttagcgattt
     4321 cttcagctgg cggagggggc acttcggtat atatcgcaat gacgatttat atactttcac
     4381 tgggtcatcg tcatattaag ccttgtccga atccggatgt aaaaaaaccg actttgcgtc
     4441 ggttttttac tttccagccc tgagttggtg gcactggctg gagtgagaat gccattatct
     4501 aacgcatcaa tgctaatacc atactgaaac tattgcaagg acatgctggt tttataacct
     4561 gaatgtactg tatgattatc cagttagctc tgaggcattt tcactctggt aatgcgcata
     4621 aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg
     4681 agtgagaatg ccattatcta acaatagggc atgtgccgtg acaggcagtg gatgagtaag
     4741 cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt
     4801 cttggggcgt taattgcgct gctggaactg atccgctttc tgcttcagct tctgaactga
     4861 tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc
     4921 attgtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt
     4981 ttggttgcac ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag
     5041 ccaatgccgt gaataccgac aatcttctct tccgatccaa cgcacaccag cttcatgcgg
     5101 cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact
     5161 ttcacctgat cgtcgccata ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca
     5221 atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc
     5281 ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagc
     5341 tccactgcac ccgtgttatc gcccaccgcg taaatacctt caatattggt gttttgatat
     5401 ttatcgacga cgatatagcc tttttcgtta gttttaacgc cagcggcttc caggttgatg
     5461 ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga
     5521 ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg
     5581 gcgttggtgt gcagctgcgg gccttcggcg ttcatcactt cgaccagcgt ttcggaaatc
     5641 atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg
     5701 aggccgttaa tcacgcccgc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg
     5761 cgctctggca aagcaggaag ggcgaagaag ccatcagaat caataccgta ttccacgccc
     5821 ggaatatccg ggtggctcgg acgaccgcct gtggcgatca gaatatgatc ggccgtgatg
     5881 gtttcgccgt ttacctccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca
     5941 tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg
     6001 gtacggctgg cgatcaacgt ttcccagttg aatttattga tagtggtatc aaaaccataa
     6061 tccgggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc
     6121 ggcacacagc caacatttac gcaggtgccg cccagctctt tggcttcaat cagcgcacat
     6181 ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg
     6241 gcgatgtaat catagtgttt agtcatgaca aagtgtcctt atcgttgatt accgcgattg
     6301 tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta
     6361 cggtggcgtg cccggtgcct gccggaacca gtttgctgtg cagccacggc agcacgttat
     6421 tcatctgctg ttccagtttc cacggcgggt taatcacaat catgccggaa gcggtcatgc
     6481 cacggcgatc gctgtctggc agtaccgcca gttcaatttg cagaattttg cgaataccgg
     6541 tcgcttccag atcgtggatc atgcgcttaa tttgctgacg cagcaccacc ggataccaca
     6601 gtgcgtaagt accagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct
     6661 gatagtcggt tttcatttca tacggcgggt cgataaggat taaaccacgg cgggaaaccg
     6721 gcggcagttt ggccttaagc tgctggaaac catcggcttt ttcgacgcgc gcacggctgt
     6781 ctttctgaaa ttcagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     6841 ggctgtcttg ttcgcgcagt agctggcgag caatcaatgg cgaaccgggg tagtaacgca
     6901 actgaccgct acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca
     6961 aatcgtcctg ctgccagata cgggcgatgc cttcgagata ttcgccggta cgctcggcat
     7021 gttcgctgcc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     7081 tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg
     7141 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     7201 gtaaaaatcg gcccgtattc ggggcgcaca aaagcttatc aggacagtat accgaaaaga
     7261 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     7321 acacacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     7381 gaatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     7441 aacatgtcgt tccagccgtg actaaggcgc tgaacgactg ccgcgaaaat gtggagcgcg
     7501 tagtagcgca aggagcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg
     7561 acgatgtgct ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc
     7621 cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac agcacctggg
     7681 tagggcaaca tgaagggctg tataaagcgt atcgcgacct gcgcgatggc gatcattacg
     7741 ccacgctgaa cacggctcag aaaaaagcgg ttgataacgc actgcgcgac ttcgaactct
     7801 ctggcatcgg tctgccaata gagaaacaac aacgctacgg tgagattgcc actcgtcttt
     7861 ccgagctggg caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac
     7921 tcgttaccga cgaagcggag ctggcgggga tgcctgaaag cgcgctggct gcggcaaaag
     7981 cccaggccga agcgaaagag ctggaaggct acctgctgac gctggatatc ccaagctacc
     8041 tgccggtaat gacctactgc gacaaccagg ccttgcgtga agagatgtat cgcgcttaca
     8101 gcacccgcgc ttccgatcaa ggcccgaacg ccggtaagtg ggataacagc aaggtgatgg
     8161 aagagatcct cgctctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct
     8221 ttaaatccct tgctactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg
     8281 atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct ggcacaactg cgcgccttcg
     8341 ccaaagcgga atttggcgtc gatgagttgc agccgtggga tatcgcgtac tacagcgaaa
     8401 aacaaaaaca gcacctctac agcatcagtg acgaacagct gcgtccgtac ttcccggaaa
     8461 acaaagcggt taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag
     8521 agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa
     8581 ataacgaact gcgcggcagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg
     8641 gggcgtggat ggatgactgc gtaggccaga tgcgtaaagc tgatggttcg ctgcaaaaac
     8701 cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca
     8761 ctcacgacga agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga
     8821 cccgcatcga aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac
     8881 tgccgagtca gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctccg
     8941 gtcactatga aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga
     9001 agaactacca ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc
     9061 gccttcatgc tgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa
     9121 tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca
     9181 gccatatttt cgccggtggt tatgcggcag gttactacag ctacctgtgg gctgacgtac
     9241 tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc
     9301 agtcgttcct cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca
     9361 aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg
     9421 gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta
     9481 tctgttctgg cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg
     9541 ttaacgccgg aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatattt
     9601 gttgattttg ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag
     9661 gcggtggcga aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc
     9721 gcagggctgg ggcgcgatgc ctttgtgctg gcttcagtcg gttgccgcgt gcggatgctg
     9781 gagcgtaatc cagtcgttgc cgcattgctc gacgacggcc tggcgcgtgg ttatgcggat
     9841 gcggaaatcg gcggctggtt gcaggagcgt ttgcagttaa ttcacgcctc cagcctgacg
     9901 gcgctaaccg atattacccc acgcccgcag gtggtctatc tcgacccgat gttcccgcat
     9961 aagcagaaaa gcgcgctggt gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg
    10021 gatcttgatg ccgatggatt gctggagcct gcacgcttgc tggcgaccaa acgcgtggta
    10081 gtaaagcgcc cggactacgc gccaccgcta gcgaatgtcg ccacgccaaa cgcggttgtg
    10141 actaaagggc atcgctttga tatctatgca ggtacgcccg tgtaaaagta aagcagggta
    10201 gcgataacgc taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat
    10261 gcgtttcagc cacggcacca tcaacagcat cactactgca acgcccagtg tgaccagacc
    10321 aatcttacca aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc
    10381 cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag
    10441 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
    10501 caggccaagg gcgctaataa acagttcacc taagctctgg aataagtaca ccagcacgat
    10561 aaaccatggc gatgtcagcc cctgggcatc cgcaaaccac attcccgcag ctgcagccgt
    10621 caaaaagccc agagagcaca taaacatgcc gagagtaaat ttcatcggca tcgagaggtc
    10681 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
    10741 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
    10801 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
    10861 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
    10921 atccagcttg aatgcctgac gaaagaagat gatggtgacg acgatggaga gaacaatcag
    10981 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
    11041 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
    11101 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
    11161 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
    11221 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
    11281 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
    11341 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
    11401 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccgagaa caatggtgcg
    11461 tttggtcccc agcaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
    11521 cgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
    11581 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
    11641 aaaaaagatc atgaaaaatg ggcgaggttg ttgcagcatc cccatgggtg ttgttgtatt
    11701 catatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
    11761 gaaaaagttt acatctgtca gagattctta atattgatgc ggtgaataga ttaatacact
    11821 attacaacag aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat
    11881 gttgtattgg caggagattt tgattgcgta cgcagatcgt aggccagata aggggtttac
    11941 gctgcatcag gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg
    12001 aacacgtcgt ggggggatga ttattcttct tcgtcgcgca gcggaacaat cagcatatca
    12061 acgtgaacgg tgttgatcag ctgacgtgcg gaagacatta gtttgctcca gaagtcctgg
    12121 tggtgaccac aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacctgg
    12181 cccaggtcgc cgctgccgct cagggtttca gtgattgggt agcctgcatt agtggaaagc
    12241 tcggtcagcg catgatgtgt ctcttcagag atgcgtttct gcatatcacc cagattcaca
    12301 tcaataagcc cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc
    12361 gcattgtagg ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag
    12421 aggtcgaccg cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt
    12481 cgatgactgg ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg
    12541 tgcgcggggc taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga
    12601 cccggatgat ccgtcaatcc gccttgctta ctcgttagat aaacaaattt tcgacatgaa
    12661 tatattgata gtggttaacc ttctggaaaa aaaacaacct gatctcctac actatctata
    12721 gagccgctcg gatgttggtc accacaaaag cggtttctcc aggacctagt gttaactgat
    12781 tggcagcgta tcgggagcgg tagccccggg gtggaactcc gcgggcaggt cgccggggag
    12841 gaggtatgat aagcaccgtc gcattatttt gggctttatg tgtcgtttgc attgttaaca
    12901 tggcgcgcta tttctcatca ctacgcgcgt tgttagtggt actgcgtaac tgcgatccat
    12961 tgctctatca atatgttgat ggagggggct tttttacctc acatggccaa cccaacaaac
    13021 aggtgcgtct cgtttggtat atctatgccc aacgttatcg cgatcatcac gatgatgagt
    13081 ttattcgccg ctgtgagcgg gtgcgtcggc agtttattct gactagcgca ttgtgtggtc
    13141 tggtggtggt cagcctgatt gcattgatga tttggcatta agcacttcag caataataac
    13201 gcataaaaaa agcgggccag taaactgacc cgctcgtttt ggtgcgtacg attacaggaa
    13261 ctgcaaggag agccagtaca gcccgccgga aagcagtacc gcagccggaa gggtaaacac
    13321 ccaggccatc agaatactgg ttacggtttt acgctgtaag ccgccgccat ctaccaccat
    13381 cgtccccgcg acagaagagg agagtacgtg agtagtggaa accggcatcc cggtataact
    13441 cgccaggccg atagacactg ccgccgtcat ctgggcagac atcccctgag cgtaggtcat
    13501 gcctttctta ccgattttct caccgatagt cgttgccaca cggcgccagc caatcatcgt
    13561 accgatacct aacgccagcg cgaccgccat gatgatccac accggtgcat actcgatggt
    13621 gctaagcatg tcggacttca gtttcttcaa caggcgctga tcgtcagcac tcacgccagg
    13681 catcttcacc actttgtcga tagtgtcaga aacgcacagc ataatgcggc gcatctggct
    13741 acgttgatca agcgacagct tgtcgtagct ttccacatcg gtggtcaaca tacctttcag
    13801 gcggttgagc gcgttaatgg tattcgacgg atggcagtgg aactccgcag gttgcgttgc
    13861 gccagcttcc ggagccggta ctaactgatc agcaccggta gcctgtttga gcagcgcagg
    13921 atgctgctca aagtaagctt cgacgttgtt gatggcatca cgggtacggg tgatttcgta
    13981 gccagtggca ttcatgttca ccacgaagcc tgctggcgcg acgccaatca ataccaacat
    14041 aaccagacca atgcctttct gaccatcgtt cgcgccgtgc gaaaacgcca cgccgatagc
    14101 ggaaaggatc agcgcgatac gcgtccagaa cggcggcttt ttcttgccgt ctttcttttc
    14161 acgctccgct ggggtcaggt ggatacgggc gcgtttcttg gtgccgctcc agtagcgacg
    14221 cagcaagaaa atcagaccgc cagcaaacac caggccgaca ataggggaaa cgatcagaga
    14281 accgaaaata cttaatactt tcgggatatt gagtgcatcc accactgacg tcccggtcat
    14341 caacgcattg gttaaaccaa tcccgatgat cgcgccaatc agcgtatgag agctggatgc
    14401 aggtaaacca aagtaccagg tacccaggtt ccagataatc gccgccagca acatagagaa
    14461 caccatggca aggccatgag acgatcccat attaagcagc agatccgtcg gcagcatatg
    14521 cacaatggca taggcaacac tcagaccacc cagcaaaaca cccaaaaagt tgaacaccgc
    14581 cgccataacc acggcgagct gagaacgcat cgcgcgggta tagataacgg ttgccacggc
    14641 gttggctgtg tcatggaaac cattgatggc ttcgtagaac agcacaaaag ccagtgcaag
    14701 caataataac agcccggtat gcaaatccag gccagcaaac aaatgtagca taggacgtta
    14761 cgccattttg aggacatgaa cgcggcgcat tatcagtgac tttcgcggcg cgggcaaagt
    14821 gaaatataga ctttttttga tttgcctcct gtatggattt cactcaaaaa ataattatct
    14881 tatataattc aggcaaatac ttccttttag taatattgat gctggtgcga ccactgagga
    14941 atctttacaa ttcacgcccg ttttttctaa gaggagcgca acgtggaaag gtttgatgcc
    15001 attattatag gcgctggtgc ggcgggtatg ttctgttctg cgctggcagg tcaggcagga
    15061 cgccgggttc tgctgatcga taatggtaaa aaaccagggc gcaaaatcct tatgtctggc
    15121 ggtgggcgct gcaactttac caacctttat gtcgaaccag gcgcttatct gagccagaat
    15181 ccgcattttt gtaagtctgc actcgcgcgt tttacccagt gggatttcat tgatctggtc
    15241 aataaacacg gcatcgcctg gcacgagaaa acgttaggac aactcttctg cgatgactcc
    15301 gcgcagcaga ttgtcgacat gctggtggat gagtgcgaga agggcaatgt aaccttcaga
    15361 ttgcgtagcg aagtgctgag tgtggcgaag gatgaaacag gcttcacgct tgaactgaac
    15421 ggcatgactg tcggttgcga aaagctggtc atcgcgaccg gtgggctgtc aatgccgggg
    15481 ctgggcgcgt cgccgtttgg ttataagatt gccgaacaat ttggcctcaa cgtgctgccg
    15541 acccgcgcgg gtctggtgcc attcactctg cataaaccgt tgctcgaaga gttacaggtg
    15601 ctggcgggcg tggcggtgcc ttccgtgatt accgctgaaa acggcatcgt tttccgtgag
    15661 aacttactct tcacccatcg cggcttgtct ggaccggcgg tgttgcagat ttccagctac
    15721 tggcaaccgg gtgaatttgt cagtatcaat ctgctaccgg atgtggatct cgaaaccttc
    15781 ctgaatgagc agcgtaacgc acatccgaat cagagcctga aaaacacact ggcggttcat
    15841 ctaccgaagc ggttggttga acgcttacag caactcgggc aaatcccgga tgtttcgcta
    15901 aaacagctca acgtgcgtga ccaacaggca ctgattagca cattgaccga ctggcgcgta
    15961 caacccaacg gcactgaagg ctatcgcact gccgaagtga cgctcggcgg cgtggacacc
    16021 aacgaactct cttcacggac gatggaagcg cgcaaagtgc ctgggctgta cttcatcggc
    16081 gaagtgatgg acgttaccgg ctggctgggg ggctataact tccagtgggc gtggtcgagt
    16141 gcgtgggctt gtgcgcagga tttgattgct gttcaaaaac aataaagttg tgccctaagc
    16201 actaaagttg taaccactct caaaaaatga cgctaatgtt ttatacttac tatatgtgtg
    16261 gttatagggt tgtagactat taaacactca tgcaggtacg tatcaatgat gttttgtggg
    16321 tacgatcatg gctaggggca ggcgtcttaa atcctatcag gattatgaaa atgcgctagg
    16381 tgacggcata gggttgggct atgggcaaag ttatcaaccc tggcttagag ctcaggacgt
    16441 tagatcccat ggaaaccgtt cgatagtctt tggccttaag acgtttcgga atcaccattt
    16501 cttttcttct gtcgaaagta actttttcta cttggctgag tttaatgact tggtgatcga
    16561 tatccgcgaa caattcccac tcatacctct actgctcacc catcagatag ccaatcactt
    16621 agatgtgcag cacccgcttg tcaggggagt cagaggcgta ccagccgatg tcctgaatgt
    16681 tatgacaact gatttcttgc tgaccctgag aatgccggga ggggggcttc ggtataaagc
    16741 aattgcagta aagcaaaacg aaagcatacc taaacgcgaa gcagaaaagc ttgagataga
    16801 gagaatgttt tggcaactga ttgatgttga gttccaaatc tatactggtt cggaactgaa
    16861 tcatgtcacg gggcagaaca tatgttgggc tacctcggtg ctgagaaatg ggtctgagtt
    16921 ttttgataaa tatccgcttg ataaaatttt atggaggctt aagccggatg tttatccgat
    16981 tgtggaacta cgcgcgatga tctcatcaat ctttgatgtc gatgaacaag aagcaatcat
    17041 gttattgcag gcaattattg ggttaaaaat gataaatgtg gatctatctt atccaattct
    17101 cgaaacaggt ctgataaaaa taatttccaa tggttactgc atagggctga acgcaaatgg
    17161 atattattag aaattcagta tggcaatctc acgacagtga cgtgctatct gatgggcttt
    17221 atcgcgttct ggattttgac agagataccg atttgttgat tttatttgaa ataaaaaaag
    17281 aaagaacgag taaaccaatt ccattctcac tttcacaatt tacatatggt gtggactcgc
    17341 aaagaataac ccataagaaa tatacacccc catcatatat gttgattgat gagagaaaaa
    17401 taaataatga agacagaaag cgacgggatg taaactataa gattattaaa gaacttgtag
    17461 aggataaaat attcgtattt gattatgcga ttcataaaaa atcacatttg ttaacagagt
    17521 actccagaaa aaaacaaatc ccacaacgta caatcaggac gcttctaact ctttactgga
    17581 aacatggaca ggatatatat gctttgctac ccgcataccc gaactgcggt gctgcaggta
    17641 agagtagaat cagacatgaa gttaagctcg ggaatgcaaa gaaaaacaga gtattaccca
    17701 atatacgttc gaaagtttat atccttaatg aaggggatat tgaaaacatc aaaaagtccc
    17761 taactaggtt tcattacaaa actcatgggg aaacgataaa gaaaacgctg gtaagacaca
    17821 ttgaattata tttcaaggat gaaataaaga aagcaaacgc tgaaaacagg gctccctaca
    17881 ttccttcttt aaaacaattt acttactgga atagaaagtt gttttcaaaa gagttctcta
    17941 taaataagaa aaactctaaa aaagaaatag ccctcaagat gcgtggcctt ctggggagtg
    18001 ccgctaatac gaccgtattg ccgggtgatg tttttgaaat agactcaact gtagctgatg
    18061 tgcaccttgt ttcaagctta aacaggcgta aagttattgg ccgaccaact atttatacag
    18121 tagtggatcg tgcttcaagg atgattgttg gtcttcatgt ttctttgtat catgcttcct
    18181 ggcgagcggc ccggcaagcg ttggcaaatt gctttatgcc aaaaaaagaa tactgtagaa
    18241 tatttggtgt ttctattaat gaagatgaat ggccttgttc tcatatacca ttaacactta
    18301 tgtgcgataa tggagagatg attggtctta aacctcaaga taagatgact cctctgacta
    18361 aacttgagtt tgcaccggtt ggcagagggg acagaaagag tattgttgag cgttgtttcg
    18421 ggattttaaa tgatgaagct attcatgagc ttacaggagc aacgcgaaga ggcaaaattg
    18481 ttaaaggaga accaacaccg caatccagag cctgtttaac gattcaggag gtcacctcag
    18541 tgctgattcg ggaaatcctt gcacataatc aaagaacgta tgaggagttg gcttatatta
    18601 atccattgtt gattgaacat gatctcatta tttcgccaaa aaacagctgg atgattagcg
    18661 taaagcaagg acggttcagc ggaagagtcg ttacagcaga tgaagtcatt gctcgtttgt
    18721 taatccccgt gacagcaaat attacagccg gtggaattca gtataataat ttattttatg
    18781 aatgcgatcc tgacattgct tcagcagcaa gagtattcgg acgaacttcc tgtgaagcaa
    18841 gaattgatga taattgcgta gattatttgt acgtaagatt cgataaaaat agtgtcttca
    18901 gaaagcatta tctactcaaa aaaagagatg tatttaaagg taaagctcat cttgatacag
    18961 atgttatggc cgactgggta gatacgcaaa aagaaataaa tgcattcaca ctgaatgttt
    19021 ttaacaatag taaagttaag gataaaatca acaggcaagg tattgaacgc ctgaacgata
    19081 tttctgatcc ccgccgggtg catggtaaag atatcaggcg gaacagaaaa gctgaactgg
    19141 acctattagg gcgtacaaat ctgcctaatg aaaaaaatga acctgtactt ccttcatcca
    19201 gtaatattct gcttttgcct ggccgtgaag aaaaacagcg atggttaaac gctaagaaaa
    19261 aacaagagga cgcagaaaaa tgaatatgtt acggagcgtc tcagcaattt ataaagagcc
    19321 gggcttgcct gaatatcggg gcaatccctt aattgaggca cttcctgaag cgctttccga
    19381 tgatgaagtg ttttttgaaa tgagttactt tcctgaaatt gacgaagata ttcgctggac
    19441 tgctcctgct aaaatcaggg agcagtatgt cgatcggata aagaaatttc gatgtccaca
    19501 aacgaatctt atcgaagctt acagaatgat ttaccgggct ttgagggaaa gttatgctgc
    19561 ccgaaatcct ttaaaaagtg gtactgttca gtacctgcat tactatggta acgagcgacc
    19621 tgacatagag ccagatagcg ggtattttaa gtctcaggct gaaactatta cgatagtcgg
    19681 gatgagtggc tccggaaaaa ccaccatgat tgagcaaatt atggactact ttccccaggt
    19741 cattgagcac agtagttata aaggtgcttt tcccgggcta agcaagcaaa tcgtgtggtt
    19801 aaagattaat tgtccgtaca actccagtgt cagagacctt tgcgaagaaa ttcttctaaa
    19861 gttagatgat gctagcggca tcgaacgaac tacaccagag atcaggaatg gtcctctggc
    19921 gaggcagata gcgcagagaa ttaaatcatc gtttttgggt attcttgtta ttgatgaaat
    19981 gcagcggctg aaattttcca gaacgggcgg tgaaaacaaa ctcattgatt ttcttcatga
    20041 aattgtagat tcaatgggtg tatcaatggt tttttgtgga aacccccctt ttgatgaaac
    20101 actggccaga aaaatgcgta ttgccagacg agcggaatcg ggtggttata tgaatattct
    20161 caatgtcagg tatgactcac aggactggca gtcgtttatt cattatttat ggccattaca
    20221 atggacaaat attgaaactc cgttaagtga tgagcttaat gaaaaacttt tcgttttatc
    20281 taaaggtaac atcggactgg ctcagatgat ttatcgcagg gcacaattaa aagttattgg
    20341 aagcggcaat gaactgatca gtagtgtagt gttgtcgtct tctgtaccgg tgctcgccag
    20401 tcatacggag gagtatcagg aggctttgat acttggagct gtgggtgggt atgatgataa
    20461 atattgctct gtatctcatg caggcgaagc ctcaacggat tttactgccg acaggcacgc
    20521 gaaggctctg attcctggag atattgacag gccacagcat aaagaatttg cacaaaaaat
    20581 tgatgaggct atgcaaaatt tcgaaagtaa aatcatatct ttcgatcggg agcgtattat
    20641 cagcagggct gctgataaca acgaaaccta ctcatcgctt cagcgttgcg gtctgttaaa
    20701 tacagaccca cttaatgaat tgtaatactg aatttattgt tcagagtggt tgctgagagg
    20761 tgggcgaatg attctacttc ctgttttacc tgatgaaacc ttgtttagcc gtatatgtcg
    20821 gtcagttact gtgtatggta tgtctccttc taccatgtta caaatccttt ttggaaaatc
    20881 tgatgttagc gtacacccaa tactcagttc cggattaaac gatatttctc ttcatacgtc
    20941 agagagtgca gagcaactct tgtatgaaca gacattgctc cctctttttt cctgggccct
    21001 gccatccagc cgcaaagaaa ttatggattt gagaacgaca ccgggcagac taaatcggtt
    21061 gtgccgtctt agtaatttct cctccgggct acagactgtt ttaaaatttt gtccggtttg
    21121 tgcacgtgaa gatgcgtacc atcatggtgt tacatactgg catcttcttc atcaacttcg
    21181 ggggattacg acctgctatc gacatcagat attacttgaa agcatctgtg ttcctccttc
    21241 gccacatatt cgcaaagggc tgatgccccc ggtttcatat attgaacatc tgagtactga
    21301 gtttgaattc gattttgcta aattttgcta cgaatttata aatatgatcc gaaggggaga
    21361 tgcaaatcat cctgattaca tggatatact gaaacaactt aatttattat cagtatcagg
    21421 aaatataaaa aaaggggttt tttattcaca tgtttatgcg aaatgtaggt tattagattc
    21481 gggttcttca gcaattttgc ctgaatccat ttcggattat cactactggg agcctttact
    21541 caggaatcaa tcatgccagc atccaacaaa gcatctgtta ctgtgctatt gtttgttaag
    21601 aataacatgg ccagtacatg ccgatatcca taaaaaatca aagagcatcg cttttgatga
    21661 tgatacagcc cagttttgcc tcctgagtga aaaacataac agtattaatt ctcttgctaa
    21721 ggaacttaac aagagtagat gttttattaa aggagtaatc cataaaaagt atgtaaagaa
    21781 ttataagtgt agcattaaaa taaacatatt aactgagttc cttattaaat ccatggctgt
    21841 gagagggttt agtttagcat caatagcaaa gaaaaactcc ttatcggaag ggagtgtttc
    21901 atcggtgatt tcttcatttt atggattatg ttcatggcgt aaaaaatgta aaagagactc
    21961 tttaagacga cgtcataaac aaaaaatcct gcggttcatg catggcctca ctggtcctgt
    22021 gactcgaaaa gtagtcaggg cgaattgtta tgcaagtttt ttctggctaa acagatttga
    22081 aagccaatgg cttgattttt atttaccaaa ggaatcacgg cgctgtaatg ttaaaagggt
    22141 caattgggat gaaagggata agttttcatt atcattaata aaaaattttt tagctcaggg
    22201 gcagtgctca ctgtcactgt caagacttga ttctgtactg gggggacatg gctggttatt
    22261 aaaaaacaga gataagcttc cagttactat ggctttgatt aatatgagta aatctaagta
    22321 gttgaatgag gttgatatga tatatgaaaa tgaattgtgg cacagttttc ttctacgtac
    22381 tcagattata tataatatac aaaattacag gaatattatc tcagcgcatg gtgcattaag
    22441 atatgatgct tacccacgac cggagttgat taatgtgtat aagctacaca gaactcagga
    22501 cttatatgat attcttacca cagtaaatgg atttatactc acttccagaa tgatttactc
    22561 tccatatgga gtttacagct attttgatgc tatggaagat gctcacaata aaggatttaa
    22621 tattataaat acatatcctg tggtaaacat ctcacgaatt cgctattgcc acagttgtat
    22681 tctggaagat attcgtatca aaggtgttgg ctatcttcga catagatggc agtttgaatc
    22741 aagatgttgc catcacaatc aaagtttata cgaagtttgc tttgataatt attctggcgc
    22801 ggttaaagga ctctgtgagt taatggtgag tgggttaaat ccttcggggt gttattcact
    22861 tgtagttgat gtttttaacc catttcagac tccggtatat ctattgccat gttctgatgg
    22921 cagagtcaga acgtggattc tggaaaataa ggatgagttg gttaattttc tgtgtagtat
    22981 ttttaaaaat aaatcttctt caggtctgaa acgtgtaatt gatgtaaaga taatg
//
LOCUS       DAZGSP010000078        22728 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZGSP010000078 DAZGSP010000000
VERSION     DAZGSP010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.078"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            42..118
                     /locus_tag="ACHK74_003423"
     tRNA            42..118
                     /locus_tag="ACHK74_003423"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:76..78,aa:Pro,seq:tgg)
     gene            265..1500
                     /gene="aslB"
                     /locus_tag="ACHK74_003424"
     CDS             265..1500
                     /gene="aslB"
                     /locus_tag="ACHK74_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP0222179.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1608..1745)
                     /locus_tag="ACHK74_003425"
                     /pseudo
     CDS             complement(1608..1745)
                     /locus_tag="ACHK74_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(2424..3620)
                     /gene="hemY"
                     /locus_tag="ACHK74_003426"
     CDS             complement(2424..3620)
                     /gene="hemY"
                     /locus_tag="ACHK74_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFP0222180.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(3623..4840)
                     /gene="hemX"
                     /locus_tag="ACHK74_003427"
     CDS             complement(3623..4840)
                     /gene="hemX"
                     /locus_tag="ACHK74_003427"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFP0222181.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PQGDTPAAAPQGE"
     gene            complement(4862..5602)
                     /gene="hemD"
                     /locus_tag="ACHK74_003428"
     CDS             complement(4862..5602)
                     /gene="hemD"
                     /locus_tag="ACHK74_003428"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFP0222182.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(5599..6540)
                     /gene="hemC"
                     /locus_tag="ACHK74_003429"
     CDS             complement(5599..6540)
                     /gene="hemC"
                     /locus_tag="ACHK74_003429"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFP0222183.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            6927..9473
                     /gene="cyaA"
                     /locus_tag="ACHK74_003430"
     CDS             6927..9473
                     /gene="cyaA"
                     /locus_tag="ACHK74_003430"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFP0222184.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(9513..9833)
                     /gene="cyaY"
                     /locus_tag="ACHK74_003431"
     CDS             complement(9513..9833)
                     /gene="cyaY"
                     /locus_tag="ACHK74_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFP0222185.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(9881..11206)
                     /locus_tag="ACHK74_003432"
     CDS             complement(9881..11206)
                     /locus_tag="ACHK74_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFP0222186.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPKMNRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTSDRQWSKWIDLPDIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDRSFNSGKQHAYFIRQLRSKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTHVREENVRKLHNRDIIIPLTDDSCRNAATDGQTLKP"
     gene            complement(11203..12591)
                     /locus_tag="ACHK74_003433"
     CDS             complement(11203..12591)
                     /locus_tag="ACHK74_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFP0222187.1"
                     /translation="MYQKQGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLD
                     KWDKVPPLTDLTTDYLYEGAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFK
                     AWGDFAADKHSLYFEGKRTDNNGGGNRLDIKTLHQVEFRPPWDPDLLGLILRDANFLY
                     INGHRLADPDSFRVLAQKSWDQRGKFSTAFNPCVAMPFGPWDTLARTRTKILINGELL
                     DADPDTFSVVRWMPGSLLTWRDKNGLQRKVLDKENLEWDEDLTKHCLDFSLLEKKVFW
                     RKGPACKQEELPGLDPEQFQPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLII
                     NKRFNAGKRHGYLLTRAEGWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRR
                     WYARDNRYIYSFDGAQLWRYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDG
                     EFIPAPRNEATK"
     gene            complement(13049..13603)
                     /locus_tag="ACHK74_003434"
     CDS             complement(13049..13603)
                     /locus_tag="ACHK74_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFP0222188.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            13953..14156
                     /gene="yifL"
                     /locus_tag="ACHK74_003435"
     CDS             13953..14156
                     /gene="yifL"
                     /locus_tag="ACHK74_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0222189.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            14193..15017
                     /gene="dapF"
                     /locus_tag="ACHK74_003436"
     CDS             14193..15017
                     /gene="dapF"
                     /locus_tag="ACHK74_003436"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFP0222190.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            15014..15721
                     /gene="yigA"
                     /locus_tag="ACHK74_003437"
     CDS             15014..15721
                     /gene="yigA"
                     /locus_tag="ACHK74_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFP0222191.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            15718..16614
                     /gene="xerC"
                     /locus_tag="ACHK74_003438"
     CDS             15718..16614
                     /gene="xerC"
                     /locus_tag="ACHK74_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFP0222192.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            16614..17330
                     /gene="yigB"
                     /locus_tag="ACHK74_003439"
     CDS             16614..17330
                     /gene="yigB"
                     /locus_tag="ACHK74_003439"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFP0222193.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            17414..19576
                     /gene="uvrD"
                     /locus_tag="ACHK74_003440"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             17414..19576
                     /gene="uvrD"
                     /locus_tag="ACHK74_003440"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFP0222194.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(19723..20487)
                     /gene="yigE"
                     /locus_tag="ACHK74_003441"
     CDS             complement(19723..20487)
                     /gene="yigE"
                     /locus_tag="ACHK74_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFP0222195.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            20649..20705
                     /gene="ysgD"
                     /locus_tag="ACHK74_003442"
     CDS             20649..20705
                     /gene="ysgD"
                     /locus_tag="ACHK74_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFP0222196.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            20857..21807
                     /gene="corA"
                     /locus_tag="ACHK74_003443"
     CDS             20857..21807
                     /gene="corA"
                     /locus_tag="ACHK74_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFP0222197.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(21849..22340)
                     /locus_tag="ACHK74_003444"
     CDS             complement(21849..22340)
                     /locus_tag="ACHK74_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFP0222198.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFKK
                     DNF"
     gene            complement(22337..>22728)
                     /locus_tag="ACHK74_003445"
     CDS             complement(22337..>22728)
                     /locus_tag="ACHK74_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFP0222199.1"
                     /translation="CIENGINSTPSTGYQPLILQSIKTKLLSSRLNKFNDFEDSFNGL
                     GISVHDISAQKISLLSFQNYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFFRIWF
                     FLQRHKDFAFKPFFTNFNTIERIENYL"
BASE COUNT         5394 a         5730 c         5878 g         5726 t
ORIGIN      
        1 gactttaaag aattgaacta aaaattcaaa aagcagtatt tcggcgagta gcgcagcttg
       61 gtagcgcaac tggtttggga ccagtgggtc ggaggttcga atcctctctc gccgaccaat
      121 tttgaacccc gcttcggcgg ggttttttgt tttctgtgca tttcgtcacc ctcccttcgc
      181 aataaacgcc cgtaataact cattgcccca cggtatgatt tcgcccttaa cgtattgaag
      241 gatgacttca ggcaaggagt gaccatgctg caacaggttc caacgcgtgc ttttcatgtg
      301 atggcgaaac cgagtggttc cgattgtaat ctgaactgtg actactgttt ttatctcgaa
      361 aaacaatccc tttaccgcga aaagccagtc acgcatatgg acgatgacac gctggaagcg
      421 tatgtccgtc actatatcgc tgccagcgaa ccgcaaaacg aagtggcttt tacctggcag
      481 ggcggcgaac caacgttact cgggctggat ttttaccgcc gtgccgtaaa gttacaggcg
      541 aaatacggtg ctggcaggaa gataagtaac agcttccaga ctaacggcgt gctgctcgat
      601 gataaatggt gtgcatttct ggcagaaaat cattttcttg ttgggttatc gctggacggt
      661 ccggctgaga tccacaatca atatcgcgtg accaaaggtg gcagaccaac gcataagctg
      721 gtgatgcgtg ccctgacgct gctgcaaaaa catcatgtcg actataacgt gctggtctgc
      781 gtcaaccgca ccagcgcgca gcaaccgttg caggtttatg attttttgtg cgatgcggga
      841 gtcgaattca tccagtttat tccggtggtc gagcgcctgg ctgatgaaac agctgccagc
      901 gatggactga aactacatgc gcctggtgat attcaggggg aactgacgga atggtcggtg
      961 cgccccgatg aatttggtga atttctggtg gcgatattcg accactggat caaacgcgac
     1021 gtcggcaaga ttttcgtgat gaatatcgaa tgggcgtttg ccaattttgt cggtgcgccg
     1081 ggtgcggttt gccatcatca gccaacctgt gggcgctcgg tgattgttga gcacaacggc
     1141 gacgtttacg cctgcgatca ctatgtttat ccgcaatatc ggctggggaa tatgcatcag
     1201 caaacaattg cagaaatgat cgattccccg caacagcagg tgtttggtga agataaattt
     1261 aagcaattac cggcgcagtg tcgcagttgt aacgtgttaa aagcgtgctg gggaggctgc
     1321 ccgaaacacc gcttcatgct cgatgccagc ggcaaaccgg ggctgaatta tttgtgtgcc
     1381 gggtatcagc gttatttccg ccatctaccg ccatatctta aagcaatggc tgatttgctg
     1441 gcgcacggtc gcccggccag tgacattatg caggcacatt tgctggtggt gaataagtaa
     1501 aaaagcggcc attttgtggc cgcctgcggt tgattgccgg atgcggcgta aacgccttat
     1561 ccggcctaca tgatcgtgca aattcaataa attgcagcgt tctgtggata ctggtttggg
     1621 tgatcgtatc cggcaaagcc tttgcgtgcg gtggcagttg acggggtatc tgctgcgctg
     1681 gccatgagag gaagagcggc ggcgacagca acaacaagaa gtttgggtga aaacgaaaat
     1741 tccatgcaaa atgctccggt ttcatgtcat caaaatgatg acgtaattaa gcattgataa
     1801 ttgagatccc tctccctgac aggatgatta cataaataat agtgacaaaa ataaattatt
     1861 tatttatcca gaaaatgaat tggaaaatca ggagagcgtt ttcaatccta cctctggcgc
     1921 agttgatatg taaggcaggt ttattatatc gcgttgatta ttgatgcttt ttttagtttt
     1981 aacggcaatt aatatatgtg ttattaattg aatgaatttt atcattcata ataagtatgt
     2041 gtaggatcaa gctcaggtta aatattcact caggaagtta ttactcagga agcaaagagg
     2101 attacagaat tatctcataa caagtgttaa gggatgttat ttcccgattc tctgtggcat
     2161 aataaacggg tagatgctca ttccatctct tatgttcgcc ttagtgcctc ataaactccg
     2221 gaatgacgca gagccgttta cggtgcttat cgtccactga cagatgtcgc ttatgcctca
     2281 tcagacacca tggacacaac gttgagtgaa gcacccactt gttgtcatac agacctgttt
     2341 taacgcctgc tccgtaataa gagcaggcgt ttttttatgc atcagaaagg tcccggaggt
     2401 gcttgcctcc gggtgagaag gaactactgt ggtgggttgt tctgcaacgt taacatcaaa
     2461 ccgtcgcgac gcatcgctgc agcttcttcc ggcttgtgca gtctgtccag cgcgtcggca
     2521 agccatgcgt aatcgtaggc gtccggacgt tgtttcagcg ccgcgcggaa ggcgagcgat
     2581 gcttcctgcc attctccgtg cttcatcagt gactggccca gtgtgctcca caacagcggg
     2641 cgatcgccga cgtttttgat ctgctggcgc agcacttttt ccagctgttc cggattgttg
     2701 gttttcagtc gaggaatcgg cagcagtagg cgatcgtcgt actggcgttt caggccatcg
     2761 atgataattt gctgggcagt atcatgatcg tcacattcaa taagatgttc cgccattgcc
     2821 acctgcaacg ctacctgatg acgcgttttc cggctttggt ttttccacca gttacgcaaa
     2881 ccttcactac cgttatcggc acgcgcctga tccatcaggc caatccatgc ctgttgttcc
     2941 agcattgcac gatgttcttc atcaccaaca tgggctttcg ccattgatgg gatgatatcc
     3001 agcagagaac tccatgcacc tgtgcggata tacgcctgtt ccgccagacg taatacttcc
     3061 ggatggcgtg gcgtaacttc cagcagctta tccacgccgt ggcgtgcagc atggttttca
     3121 ttacgggcca gttgcagacg tacacgggtg atttctaccg gaatggtgtc attgccggcc
     3181 agctctgctg cgcgttccag atgttggttg gcgcgtgctt catcaccacg ttgttgcgcg
     3241 gcttcggcag ccagtagata gttcaccacc ggttgttccg cgtgatcggc atttttcgcc
     3301 atcagctttt caacttgctg ataatcgcct tccgccagtt tcagcagcgc ctgttcggtc
     3361 tgcttacggg cacggcgacg cttacgtccg acaaaccacc cacgggtgtg tgcgcctgtg
     3421 cggaagatcc gccgcagtag ccactcaatg gcaaacagca ctaccatcgc cagaatcaaa
     3481 ataatcgcca ggcccgtgac gctggtttcg atattgtagt tgtcagtctg gatcagcacg
     3541 taaccctgat gaccggcaat catcgggcca accactatcc ccgcaatcag caaaacaaag
     3601 agtaataaca cttttagcat ggttattctc cttgcggcgc ggctgccgga gtatcacctt
     3661 gcggtgcagg tgcaggtgca ggtgcaggtg caggtgcagg tgcaggtgca ggtttagctt
     3721 ccgttgtccc cgctgccggt tgtgccagca ggttacgcac gcgggtctgc atcagttttt
     3781 ccagcatcgc ctggctttgc agggtttccg gaagatccat cgagatattt tgctggctta
     3841 actggtccac ctcgtcgagg aacgctttgg tggtggcatc atcagtatcg tagtaagctc
     3901 gtacccaggt ggagacgttc tccagcgcct ggcgataagt ctcttcctgg tgacgcggta
     3961 cagcttgtgc tgcgaccagc aggcgagagc gaatattttc gcgcagatag atatcctgat
     4021 ttggcgctaa cagcggtacg gcggtgtcat cacgacggcg aatcgtaatg aagttgtcca
     4081 taaagttctg ccagcttttt tgcagattga tacgccattc gctgatggaa ctggaaagct
     4141 cttcaccgtc ggaatccatc ggcgaaccat cgctgtcatt atcggccaga cgcaggttat
     4201 ctacctgatt tgaaagctga ttaagcttaa ggatgatgcc gtcataatcc acctgcgaaa
     4261 ctgcagaaag gctggcgata tcatcggtaa ttgcccgacg aacggtaatc agactcgggt
     4321 cattcatatc cgccaggctg gcatctgcac ttttcagcaa cgctgcagcg gtcgtgacgt
     4381 cctgatcgct ccacagcttc cgtccggcga gtttcaccag aaaatcggcc tgagccagca
     4441 gccaggtttt agcatcgctg ccggaaatgg tggcgacctt ttgttggact tcatccaact
     4501 gttttgccag cgtttcttgc tgacgattcg cctgcttaag ctgtgcagct tgttgcttaa
     4561 taatgccttc cagctcggct ttttggctct cctgggcttt ttgcaatgcc gtcagttggt
     4621 tagccagggc atcgctggtg gcggtctgat tgacggcctg ttgtttaccc cagccataca
     4681 aaccgacgcc cgccgccaga gcaatagcga tagccaccgc gctgagaatc aatgcggtat
     4741 tgttcttact ctttttttct gttgcgacag gttgtgacgt ggtgtccacg gcctccctgg
     4801 tctcttcaac cacggcggag gttttttctt gttccgtcat tatggcttcc tgttatgaga
     4861 gttattgtaa tgcccgtaaa agcgcatcgt tgtcagcgtt atcggcgacc ttaatgtctt
     4921 gccagcccag ttcccgggcg agtttcgcca aacgctcact gacgaccaat agtcgacagt
     4981 gtagtaacca gtgctcacga taccattgtg ggatcagcga ccagagttgc tgcaacattt
     5041 caccgctggt aacaacgacc atcgtcacct cgcgggattg ccagcgcatc gcttcttctg
     5101 caccatcgta atggattgcg catcgttgat aacattcaca aaaagtgacc tcagcaccgc
     5161 gcgctgtcag ggtatcccca attagctcac gaccgccatt gccacgtaat atcagcgcac
     5221 gtttgcccgc aatattttgt aattcaggta attgtagcaa gacttcgctg atttcccgat
     5281 cctgcgggta gagaatcttc tgtccgctta cggtatgtag cgccagtgcg gtggtgcgtc
     5341 caatggcgaa ataatcaggt agtcggggcc atttacgatc ttgctgatgc agctgtgatt
     5401 gggcaaaagc aaccgcgtgt tgcgagaggg caaacaacag atcgctctcc cccagcgccg
     5461 ccagttgatc agcaagttgc ggtaattgtc gacccggaga aaactcaatc agcggaaaat
     5521 gccaggccac ctgccccagt gtgcgcagac ggctcactaa ctcttctcca gcgggagacg
     5581 ggcgggtgac caggatactc atgccggggc gtctccgtta tagacttcag cgaggatctc
     5641 gcgcgcgccg ttattcagta gctcttctgc cagcgaaatc cccatttgtt cggcattttg
     5701 cggcgcaccg cggcgttcac cgcgaataat ctgcgaaccg tccggcgcgc cgaccagcgc
     5761 acgcagccag atttcaccat caataagctc ggcgtagcta ccaattggca cctgacatcc
     5821 accttcgaga cgggtattca tggcgcgttc tgcggtaacg cgcagtgcag tttcgtggtg
     5881 attcagcgcg gcaagcagct cgcgagtgcg cgtatcatca aggcggcatt caataccgac
     5941 tgcaccttgt cctaccgccg gaagagaaat ctcgggtggt aacgcggcgc gaatgcggga
     6001 ctccagacct aaacgtttta gtccggctac ggcaagaatg atggcatcgt attcgccgtt
     6061 atctagtttg ctcaggcgag tgccgacgtt gccgcgcagg gagcggataa tcagatccgg
     6121 gcggcgttca gccagttggc actggcgacg taaactggac gtcccgacga tactgcctgc
     6181 cggtaacgca tccagactgt cataattatt ggacacaaag gcatcgcgag gatcttcacg
     6241 ctcacaaata gtgaccaacc ccagaccttg cgggaattca accggcacat ctttcattga
     6301 atgtacggcg atatcggcgc gattctccag gagcgcgact tccagctctt ttacaaataa
     6361 gccttttccg cctactttcg ccagcggcgt atcaagaatc acatcgccgc gcgtcaccat
     6421 cggtaccagt tcaaccacca ggcccggatg gctcgccatc aacttgtctt tgacatagtg
     6481 tgcctgccag agtgcaagtg ggctttggcg tgtggcaatt cttaaaacat tgtctaacat
     6541 gcttgttacc gtcattatca tccgtggtcc atcctaacat ccttgccaga gtgatgtcag
     6601 tgttgtggtg aaacgtagac gcctgcgcaa accgtaaaat gaggtctggc agtggatcct
     6661 gacaggcgtt tcacgccgtc gtaataagga atttacagag aataaacggt gctacacttg
     6721 tatgtagcgc atctttcttt acggtcaatc agcaaggtgt taaattgatc acgttttaga
     6781 ccattttttc gtcgtgaaac taaaaaaacc aggcgcgaaa agtggtaacg gttacctttg
     6841 acatacgaaa tatcccgaat gccgcgtgtt accgttgatg ttggcggaat cacagtcatg
     6901 acgggtagca aatcaggcga tacgtcttgt acctctatat tgagactctg aaacagagac
     6961 tggatgccat aaatcaattg cgtgtggatc gcgcgcttgc tgctatgggg cctgcattcc
     7021 aacaggtcta cagtctactg ccgacattgt tgcactatca ccatccgcta atgccgggtt
     7081 accttgatgg taacgttccc aaaggcattt gcctttacac gcctgatgaa actcaacgcc
     7141 actacctgaa cgagcttgaa ctgtatcgtg gaatgtcagt acaggaccca ccgaaaggtg
     7201 agcttccaat tactggtgta tacaccatgg gcagcacctc gtccgtaggg caaagttgtt
     7261 cctctgacct ggatatctgg gtctgtcatc aatcctggct cgatagcgaa gagcgccagt
     7321 tactacaacg taaatgtagt ctgctggaaa gctgggccgc ctcgctgggt gtggaagtca
     7381 gcttcttcct gattgatgaa aaccgcttcc gtcataatga aagcggcagc ctggggggcg
     7441 aagattgtgg ctccacccag catatactgc tgcttgacga attttatcgt accgccgtgc
     7501 gtctcgccgg taagcgtatt ctgtggaata tggtgccgtg cgacgaagaa gagcattacg
     7561 acgactatgt gatgacgctt tacgcgcagg gcgtgctgac gccaaatgaa tggctggatc
     7621 tcggtggctt aagctcgctt tctgctgaag agtactttgg tgccagcctt tggcagctct
     7681 acaagagtat cgattcccca tacaaagcgg tactgaaaac actgctgctg gaagcctatt
     7741 cctgggaata cccgaaccca cgtctgctgg cgaaagatat caaacagcgt ttgcacgacg
     7801 gcgagattgt atcgtttggt ctcgatccat actgcatgat gctggagcgt gttactgaat
     7861 acctgacggc gattgaagat tttacccgtc tggatttagt acgtcgctgc ttctatttaa
     7921 aagtgtgcga aaagctcagc cgtgaacgcg cctgcgtagg ctggcgtcgc gcagtgttga
     7981 gccagttagt gagcgagtgg ggttgggacg aagctcgtct ggcaatgctc gataaccgcg
     8041 ctaactggaa gattgatcag gtgcgtgagg cgcacaacga gttgctcgac gcgatgatgc
     8101 agagctaccg taatctgatc cgctttgcgc gtcgcaataa ccttagcgtc tccgccagtc
     8161 cgcaggatat cggcgtgctg acgcgtaagc tgtatgccgc gtttgaagca ttaccaggta
     8221 aagtgacgct ggtaaacccg cagatttcac ccgatctctc ggaaccgaat ctgaccttta
     8281 tttatgtgcc gccgggccgt gctaaccgtt caggttggta tctgtataac cgcgcgccaa
     8341 atattgagtc gatcatcagc catcagccgc tggaatataa ccgttacctg aataaactgg
     8401 tggcgtgggc atggtttaac ggcctgctga cctcgcgcac ccgtttgtat attaaaggta
     8461 acggcattgt cgatttgcct aagttgcagg agatggtcgc cgacgtgtcg caccatttcc
     8521 cgctgcgctt acctgcaccg acaccgaagg cgctctacag cccgtgtgag atccgccatc
     8581 tggcgattat cgttaacctg gaatatgacc cgacagcggc gttccgcaat caggtggtgc
     8641 atttcgattt ccgtaagctg gatgtcttca gctttggcga gaatcaaaat tgcctggtag
     8701 gtagcgttga cctgctgtac cgcaactcgt ggaacgaagt gcgtacgctg catttcaacg
     8761 gcgagcaatc gatgatcgaa gccctgaaaa ctattctcgg caaaatgcat caggacgccg
     8821 caccgccaga tagcgtggaa gtcttctgtt atagccagca tctgcgcggc ttaattcgta
     8881 ctcgcgtgca gcaactggtt tctgagtgta ttgaattgcg tctttccagc acccgccagg
     8941 aaaccgggcg tttcaaggcg ctgcgcgttt ctggtcaaac ctgggggttg ttcttcgaac
     9001 gcctgaatgt atcggtacag aaactggaaa acgccatcga gttttatggc gcgatttcgc
     9061 ataacaaact gcacggcctg tcagtgcagg ttgaaaccaa tcacgtcaaa ttaccggcgg
     9121 tggtggacgg ctttgccagc gaagggatca tccagttctt tttcgaagaa acgcaagacg
     9181 agaatggctt taatatctac attctcgacg aaagcaaccg ggttgaggtg tatcaccact
     9241 gcgaaggcag caaagaggag ctggtacgtg acgtcagtcg cttctactcg tcatcgcatg
     9301 accgttttac ctacggctca agcttcatca acttcaacct gccgcagttc tatcagattg
     9361 tgaaggttga tggtcgtgaa caggtgattc cgttccgcac aaaatctatc ggtaacatgc
     9421 cgcctgccaa tcaggatcac gatacgccgc tattacagca gtatttttcg tgatgaacgt
     9481 gccggaaagc gaggcttatc cggcatgcaa tcttagcgga aactgactgt ttcacccgcc
     9541 tgctgcgtcg ccgcctgttc cagcaaatcc cagaaggttt cgccgctgcg atcgcaaatc
     9601 cactcatcgc cttttaggtc aaaatggtag ccgccctgtt tggttgccag ccacacctgg
     9661 tgcagcggct cctggcggtt aataatgatt ttgctgccgt tttcaaaggt aatggtcagt
     9721 acgccgccgt tgatttcgca gtcgatatcg ctgtcgccat cccagtcgtc caggcgttct
     9781 tcaatggtca gccagagttg atcagccagg cgatgaaatt cactgtcgtt cattgttgta
     9841 tcctgtcttt aagtgatggc ggcagtatag cggtatgggg tcagggcttc aaagtttgcc
     9901 cgtctgttgc tgcgttccgg catgagtcat ccgtcaacgg aataataatg tctctgttgt
     9961 gaagtttacg cacattctct tctctgacgt gtgttttcgc cgggcagggg gagggcgtaa
    10021 tccttacctc tgtatcggtc catgtataaa cattgcgatc gtcataagcg aagcgatcgt
    10081 ttttagttag cggaccgtta actggaataa tttgcacgct ctttgaccgt aattggcgga
    10141 taaaataagc atgctgtttg ccgctattaa atgagcgatc aatgaccagg tctggcgtat
    10201 ccagcgggat tatctcaagc tggtcttcac caaatgtcga taattttgtt acatacaacc
    10261 gatctttata ttgcgcaata ttgccagtaa tcagatggaa ttgttcaggt tcaatatctg
    10321 gtaggtctat ccacttgctc cactgacggt cggacgtgag ctgtttacgc cagcgaacgc
    10381 gactttcccc tacttcaaaa gagacgtcat catctacggg tatcgctttg ccagacaatt
    10441 gcccaaaggg ataacgatgc atacccttat tgtcgcgaaa aaccagcagt gaatgaggta
    10501 tccagcgaat tatctcaaat gagtctgcat tcgccgtaag gtgaacgcca tttaaataaa
    10561 tattctgccc actgcggata agcagatcat tggaatacaa atttctgttt acactcataa
    10621 gacgtggatt aataccccac catctttttt ctaatacagt aacattactg ctgctcccct
    10681 gacgtcgacc ggacaaatag agatttttac tatccaccag cgtggtagag ttaccttcga
    10741 tagcttttag tgtcgccaga tcaacacgtg acgcgccgct attactttcg gtgcgctgtc
    10801 cgtcaaaata gaggctatat ttatcgaccg cgaagcggcc catagcctga aagctggcaa
    10861 tatcgacagt gggtgttccc ggtggattgc taacgatttt gccacgccag agaataacgc
    10921 ggccgtctgt cacccagtgg tattcctcat tttttatgct tatttcatcg gtgggtatat
    10981 agccttccac caactcccgc tccccggata tatttgtcac aaggcgcaaa tcggctctgt
    11041 tcatcttcgg aaattgggca atgattttct tatcccccca ataaccttta ccgtaagaca
    11101 taacaccgtg atcattgtaa tagtcgaacc ctatggcctg tacagcaggc gtcagaaatg
    11161 tgtgtcccaa caggatgagt gcaactgtga ttatttgacc cttcattttg tcgcctcatt
    11221 gcgcggggca ggaataaatt cgccgtcatc agtaattttt ccgtccagat atcccgtttt
    11281 gtaatttacg ccatagccat atcccgaatg ttgttccttc cagattaagc gaacttgttt
    11341 cggatcagcg gtgcggtatc gccagagctg cgcgccatca aagctgtaaa tgtagcggtt
    11401 atcgcgggca taccatctgc gcaaaaaggg atggtcatta agatgggctt cgcgttcatc
    11461 cggagaacgg ttatccagta agatcagcgg tccgtcagat tcaaacacat gtaaaccgga
    11521 atggtatggc cacccttcgg cacgcgtaag taaatagccg tggcgtttcc cggcgttgaa
    11581 acgtttgttg ataataagat tgggatcatc aagtttcacg atttccagct tgcggtcacc
    11641 agactctgtt tcgatgatgg tataaagcga gtcctgatac tgggcgacag catcactgat
    11701 gggttgaaac tgctccggat cgagtccggg taattcttcc tgtttacaag caggtccttt
    11761 acgccagaac actttctttt ccagcagaga aaaatccaga cagtgctttg ttaaatcttc
    11821 atcccactcc agattttcct tgtcgaggac tttacgctgc agcccgtttt tatcacgcca
    11881 ggtcaagagt gagccgggca tccagcgcac gacggaaaag gtgtccgggt cggcatcaag
    11941 cagttcgccg ttgatcagga ttttggtccg tgtacgagcc agggtatccc aggggccaaa
    12001 tggcatagcc acacagggat taaacgcggt agaaaacttt cctcgctgat cccatgactt
    12061 ttgtgccagc acacggaaac tatcgggatc tgcgaggcga tgcccattga tatacaagaa
    12121 attagcgtca cgtaatatca gccccagtaa gtcaggatcc cagggaggcc ggaattccac
    12181 ctgatgcagt gttttgatat ctaagcgatt tcccccgcca ttgttatcgg tgcgcttgcc
    12241 ttcgaaatag agactgtgtt tatctgcggc aaaatcaccc cacgccttga acgaggcgac
    12301 atcgacggga ggtttgtctg gcgggttttg cacaacttca cccgcgtaga ggatatagcg
    12361 accatcgcta tgccaggagt aatgaggata ccaggcctgt gcgccttcat agaggtagtc
    12421 ggttgtcaga tcagtcagag gaggtacttt atcccatttg tctagtgttg tcgggtctat
    12481 taataaattg ggtaaacggc gcaaactttt gtaattcacg tcaggaagag aaagatttgt
    12541 ttctttataa gggcgcaggt ataccacgcc ttttccctgt ttctggtaaa tattatgttc
    12601 aggcggatca agccgacatc cccatgccga cgataaaaac tatgaacaag acaacaaaca
    12661 aaccacattg cgatagtgca taaagccatc ctggcgcgag gtgccgatca cgaaactacc
    12721 agcagaacat aattcctcgc acgtaagcgt aacttttgcg gcaaatcgtc acttttcaga
    12781 atctataaag gccatcgcta ttttctgata gttgctgggg taaacttgag gttttcattc
    12841 acgatataaa tacgagctac tgcgccatgg tgtgactgat cacgttaaaa tgtgccggat
    12901 tgacgccggg aagggtgtca acatgcgact ctgcccaccg ttacctactt tattgtcttt
    12961 cgcgtgttgt acttaggtcc caccaccggt atctaacatt ccccacatta ttacgctttc
    13021 tgccgaacgt gatttttata gaaatctatc aattcatttt ccctgtacca taaagtttat
    13081 ctttcacatc ctgccgaacg cgggtcttgt accccccgtt gtgaagatct tcatacggag
    13141 aaaaagaatc tttactccac cccagcagca ctttatcgcc aggcataaaa tgcacatgca
    13201 gaaaatcttc tccccattct cttttgggga gaggtaacgt catattccgt ttctccagac
    13261 gcattccagc attatactgt gtacgagtag tacttaatgt ccagataacc tctgccgtat
    13321 ccccactaac atcaccacaa caggttgttg aaccgccacc atttgcaaaa gcattcgcgc
    13381 ccgccacgcc gttaacactg aatcccagta tcgggcggtc aatttcactg tggatttcca
    13441 gaatcactcc acctcttggt cctccccata ctgcctgtcc tatattccac aacacgacca
    13501 gtaccggaac gacaattaac gtccgccata cccagcgccc ccaccgggca taaccgcgcg
    13561 taagcttttg gtcaaatttt tccagttggg taaatagccc catcagattt tattccctct
    13621 tatcaccagg ttggcaaagt tgtatgtcac tggcgactaa ggtcagacaa cgaaattgca
    13681 tggtgcgcca ctctattaaa agccccatgc cgacgataag aacaatgaac aagacaacaa
    13741 acaaaccaca ttgcgatagt gcataaagcc atcctggcgc gaggtgccga tcacgaaact
    13801 accagcaaaa cataaatccc cacgtgtaag cgttatactc gcagcatttc ctcacttttc
    13861 agacttcata aagagtcgct aaacgcttgc ttttacgcct tctcctgcga tgatagaaag
    13921 cagaaagcga tgaactttac aggcaatcca taatgaaaaa cgtgtttaag gcactcactg
    13981 tattaattac tctcttcagc ctgacgggct gcggtctgaa aggtccgctc tatttcccgc
    14041 ctgcagataa aaacgcaccg ccgccgacca aaccggtaga gacgcaaacg caatccacgg
    14101 tgccggataa aaacgatcgc gccactggcg atggtccatc ccaggtgaat tactaaaagt
    14161 cagtttctgt acccgcgtga ttggagtaaa tgatgcagtt ctcgaaaatg catggccttg
    14221 gcaacgattt tatggtcgtc gacgcggtaa cgcagaatgt ctttttttca ccggagctga
    14281 ttcgtcgcct ggctgatcgg cacctgggag tagggtttga ccaactgctg gtggttgagc
    14341 cgccgtatga tcctgaactg gattttcact atcgcatttt caatgctgat ggcagtgaag
    14401 tggcgcagtg cggcaatggt gcgcgctgct ttgcccgttt tgtgcgtctg aaagggctga
    14461 ccaataagcg cgatatccgc gtcagcaccg ccaacgggcg gatggttctg accgtcaccg
    14521 atgatgatct ggtccgcgta aatatgggcg aacccaactt cgaaccttcc gccgtgccgt
    14581 ttcgcgctaa caaagcggaa aagacctata ttatgcgcgc cgccgagcag acaatcttat
    14641 gcggcgtggt gtcgatggga aatccgcatt gcgtgattca ggtcgatgat gtcgataccg
    14701 cggcggtaga aacgcttggt cctgttctgg aaagccacga gcgttttccg gagcgagcca
    14761 atatcggttt tatgcaagtg gttaagcgcg agcatattcg tttacgcgtt tatgagcgtg
    14821 gggcaggaga aacccaggcc tgcggcagcg gcgcatgtgc ggcggtggcg gtagggattc
    14881 agcaaggttt gctggccgaa gaagtacgcg tggaactccc cggcggtcgt cttgatatcg
    14941 cctggaaagg tccgggtcac ccgttatata tgactggccc ggcggtacat gtctacgacg
    15001 gatttattca tctatgaagc aaccagggga agaactgcag gaaacactca cggagcttga
    15061 tgaccgggcg gttgtcgatt atctgattaa aaatcctgag ttttttatcc gtaatgcgcg
    15121 cgcagtagaa gcgatacgtg tgccgcatcc ggtacgcggc accgtttcgt tggtcgagtg
    15181 gcacatggcc cgcgcacgta atcatattca tgttctggaa gagaacatgg cgctgttgat
    15241 ggaacaggct atcgccaacg aaggcctgtt ttatcgccta ctctacctgc agcgcagtct
    15301 caccgccgcc agcagtctcg acgatatgct gatgcgcttt caccgctggg cgcgcgatct
    15361 cggcctggca ggtgcgagtc tgcgcctgtt tccggatcgc tggcgcttag gtgcgccgtc
    15421 gaaccacact catctggcat taagccgtca gtctttcgaa ccgctgcgta ttcagcgttt
    15481 ggggcaggaa cagcactatc ttgggccgct taacggacca gagctgctgg tggtgctacc
    15541 ggaagcgaaa gcggtgggat cggtggcgat gtcgatgctg ggaagcgatg ctgatttggg
    15601 tgttgtgctg tttaccagtc gcgatgccag tcactatcaa caagggcaag gaacgcagtt
    15661 acttcatgaa attgcgctga tgttgccgga gcttctggag cgttggattg aacgcgtatg
    15721 accgatttac acaccgatgt agaacgctac ctacgttatc tgagcgtgga gcgccagctt
    15781 agcccgataa ccctgcttaa ctaccagcgt cagcttgagg cgatcatcaa ttttgccagc
    15841 gaaaacggcc tgcaaagctg gcagcaatgt gatgtgacga tggtgcgcaa ttttgctgta
    15901 cgcagtcgcc gtaaagggct gggagcagca agtctggcgt tacggctttc tgcgctacgt
    15961 agcttttttg actggctggt cagccagaac gaactcaaag ctaacccggc gaaaggagtt
    16021 tcggcaccga aagcgccgcg tcatctgccg aaaaacatcg acgtcgacga tatgaatcgg
    16081 ctgctggata ttgatatcaa cgatcccctc gctgtacgcg accgtgcaat gctggaagtg
    16141 atgtacggcg cgggtctgcg tctttccgag ctggtggggc tggatatcaa acacctcgac
    16201 ctggagtctg gtgaagtgtg ggtaatgggg aaaggcagca aagagcgccg cctgccgatt
    16261 ggtcgcaacg ctgtggcgtg gattgagcac tggcttgatt tgcgcgacct gtttggtagc
    16321 gaagacgacg cgctttttct gtcgaaactg ggcaagcgta tctccgcgcg taatgtgcag
    16381 aaacgctttg ccgaatgggg cataaaacaa gggctgaata atcacgttca cccacataaa
    16441 ttacgtcact cgttcgctac gcatatgctg gagtcgagcg gcgatcttcg tggtgtgcag
    16501 gagctgctgg gtcatgccaa cctctccacc acgcaaatct atactcatct tgattttcaa
    16561 caccttgcct cggtgtacga tgcggcgcat ccacgcgcca aacgggggaa ataatgcgtt
    16621 tttaccggcc tttggggcgc atctcggcgc tcacctttga cctggatgat accctttacg
    16681 ataaccgtcc ggtgattttg cgcaccgagc gagaggcgct tacctttgtg caaaattatc
    16741 atccggcgct gcgcagcttc cagaatgaag atctgcaacg cctgcgccag gcggtacggg
    16801 aagcggaacc cgagatttat cacgacgtga cgcgttggcg ttttcgttcg attgaacaag
    16861 cgatgctcga cgccgggctg agtgccgaag aagccagtgc aggcgcacac gcagcaatga
    16921 ttaactttgc caaatggcgc agccgaatcg acgtcccgca gcaaactcac gacaccttaa
    16981 aacagctggc gaagaaatgg ccgctggtgg cgatcaccaa cggtaacgcc cagccggaac
    17041 tgtttggttt gggggattat tttgagtttg tgctgcgcgc tggcccgcac gggcgctcaa
    17101 aaccgttcag cgatatgtac tttttggctg cggaaaaact caacgtgccg atcggcgaga
    17161 tcttacatgt tggggacgat ctcaccactg acgtgggtgg ggcaattcgc agcggaatgc
    17221 aggcttgttg gatcagaccg gaaaatggcg atctgatgca aacctgggac agccgtttac
    17281 tgccgcatct ggaaatttcc cggttggcat ctctgacctc gctgatataa tcagcaaatc
    17341 tgtatatata cccagctttt tggcggaggg cgttgcgctt ctccgcccaa cctattttta
    17401 cgcggcggtg ccaatggacg tttcttacct gctcgacagc cttaatgaca aacagcgcga
    17461 agcggtggcc gcgccacgca gcaaccttct ggtgctggcg ggcgcgggca gtggtaagac
    17521 gcgcgtactg gtgcatcgta tcgcctggct gatgagcgtg gaaaactgct cgccatactc
    17581 gattatggcg gtgacgttta ccaacaaagc ggcggcggag atgcgtcatc gtatcgggca
    17641 actgatgggc accagccagg gcggcatgtg ggtcggcacc ttccacgggc tggcgcaccg
    17701 cctgctgcgt gcgcaccata tggacgccaa tctgccgcag gatttccaga tcctcgatag
    17761 cgaggaccag ctgcgcctgc ttaagcgtct gatcaaagcc atgaacctcg acgagaagca
    17821 gtggccgccg cgccaggcaa tgtggtacat caacagccag aaagatgaag gcctgcgtcc
    17881 gcatcatatt caaagctacg gtaatccggt ggagcagacc tggcagaagg tgtatcaggc
    17941 gtatcaggaa gcgtgtgacc gcgcgggcct ggtggacttc gccgagctgc tgctgcgcgc
    18001 tcacgaactg tggcttaaca agccgcatat cctacaacac taccgcgaac gttttaccaa
    18061 tatcctggtg gacgaattcc aggataccaa caacattcag tacgcgtgga tccgcctgct
    18121 ggcgggcgac accggcaaag tgatgatcgt cggtgatgac gaccagtcaa tctacggctg
    18181 gcgcggggcg caggtggaga atattcagcg tttccttaat gatttccccg gtgccgaaac
    18241 tattcgtctg gagcaaaact accgctctac cagcaatatt ctgagcgccg ctaacgccct
    18301 gattgaaaac aataacgggc gtctgggtaa aaaactgtgg accgatggcg cggacggtga
    18361 gcctatttcc ctctattgcg cttttaacga actcgatgaa gcgcgttttg tggttaaccg
    18421 catcaaaacc tggcaggaca acggcggggc gcttgccgag tgcgccattc tctaccgcag
    18481 taacgcccag tcgcgtgtac tggaagaagc actgttgcag gccagtatgc cgtaccgtat
    18541 ttacggcggg atgcgcttct tcgaacgcca ggaaatcaaa gacgcgctct cgtatctgcg
    18601 tctgattgcc aaccgtaacg acgacgcggc gtttgagcgc gtggtgaata cgccaacgcg
    18661 gggtattggt gaccggacgc tggacgtggt acgtcagaca tcgcgcgatc gccagttaac
    18721 actctggcag gcatgtcgtg aactgttgca ggaaaaagcc ctcgccgggc gagctgccag
    18781 cgccttgcag cgatttatgg aattaatcga tgccttagcg caagaaactg ccgatatgcc
    18841 gctgcatgta cagactgacc gggtaattaa agactccggc ctgcgcacca tgtacgagca
    18901 ggagaagggc gaaaaaggcc agacgcgtat cgaaaactta gaggaactgg tgacggcaac
    18961 gcgccagttc agctacaacg aagaagatga agatttaatg ccgctgcagg cattcctctc
    19021 ccatgcggcg ctggaagcgg gcgaggggca ggcggatacc tggcaggatg cagtgcagtt
    19081 gatgacgctg cactcggcga aaggcctgga gttcccgcag gtgtttattg tcggtatgga
    19141 agagggtatg ttcccaagcc agatgtcgct ggatgaaggc gggcgtctgg aagaagaacg
    19201 ccgtctggcc tacgttggcg taacccgcgc gatgcagaaa ctgacgctga cctacgcgga
    19261 aactcgccgt ctgtatggca aagaggttta ccatcgcccg tcgcgcttta ttggcgagct
    19321 gccggaagag tgtgtggaag aggtgcgcct gcgcgccacg gtaagccgcc cggtcagcca
    19381 tcagcggatg ggtacgccga tggtcgagaa cgacagcggc tacaagctcg gccagcgcgt
    19441 acgccacgct aagtttggtg aaggcaccat tgtcaatatg gaaggcagcg gtgagcatag
    19501 ccgtttgcag gtggcatttc agggccaggg tattaaatgg ctggtggcgg cttatgcccg
    19561 gctggagtcg gtgtaacgtt gccggatgcg gtgctgcgca ccttatttgg cctaaaaaat
    19621 cattcagatt caataaattg caacgtcatg taggccggat aaggcgttta cgccgcatcc
    19681 ggcatcagca gcatatttag tatctgacac aaaactatca ttctaacctt tccgctcgac
    19741 ggaaatcatg gtgacaaaag gatagcgttg ccacgggatt gccccgcctt tcatatacat
    19801 atgtgaaatc gtgccatcaa gataaagcag ctgctcaacg ttcagtttcg ctttggcata
    19861 acaggcaaaa tcataaaaat ttgtcgcctg ctggctcaac aaaaacacgg cattcccatg
    19921 tttattaatc ccaacaccgt tacgaatttt gcgtgaggcg acgttgggat gaatacgcgg
    19981 attaattaca ccgttttcca tcaacattgg ccctgactgc accgcaaact gaatctcttt
    20041 actggttttg aaggcatcca gacgaacgat gccgacttta tctcccgcga cataaaacac
    20101 gccgccagga cggataaaga aattcccttc acctgaagcg agatttaacg ccaccttctg
    20161 ctgaccgttt tcgatgtaca aaccgagcgg cgcatagctt tcatcataga tgccgccgtt
    20221 catcgccatc tgcacctgac cctgactatt catatccgcc agcagagcat gtaacgttcc
    20281 ccacgcttcg ccattagctt tttgccagta cattttcacc cgctctgttt gaggattaac
    20341 ggtatacgcc tgtacggtca gcgtcggatc tgagagtgcg caatcatcag cggcaacagc
    20401 aaacaaggga agtaaggtga gggtgagaaa aatccgtttg agattcaagg tgatcattcc
    20461 tttaccaatg agtagccgat gcgccattat aggtcctgga tgttggattt ttttatcctg
    20521 ttagcgacct tgacgagtac caaaaagcgc gaagttcaac tattgttctg tggtgttctg
    20581 ttgcgtgttg acggcaaaat tttgctggcg taacatgcgc gcacgatcac tctaagagga
    20641 cattcgcctt ggacacaccc agtagatact ggctcactat cctgtcatcc aggatcaact
    20701 cctaaggcta tccctttttg ctgatagcct tagcggttgt cagcgacctc aatttttccc
    20761 gtcgcgctga gtcaggctgt ttaatggtct gaaacccaat ttgtttctgt gtgcccaccg
    20821 aactgtccga tattttaagc attgggagtc ccggtcatgc tgagcgcatt tcaactggaa
    20881 aataaccgac tgacccggct ggaagtcgaa gagtcacaac cccttgtaaa tgcagtatgg
    20941 attgatcttg tcgaaccgga cgacgacgag cgactgcgcg tacaatctga acttggccag
    21001 agcctggcaa cccgcccgga actggaagac atcgaagcat cggcacgttt ctttgaagac
    21061 gacgacggcc tgcatattca ctccttcttc ttctttgaag atgcggaaga tcacgccggt
    21121 aactccactg tggcatttac catccgtgat ggtcgtctgt ttactctgcg tgagcgtgaa
    21181 ctgcccgctt ttcgtctgta tcgtatgcgt gcccgtagcc agtcgatggt agacggtaac
    21241 gcctacgagt tgctgctgga tctgttcgaa accaaaatcg aacagttggc agatgaaatt
    21301 gaaaatatct atagcgacct ggagcagttg agccgggtga ttatggaagg gcatcagggc
    21361 gatgagtacg acgaggcgct ctccacgctg gcggaactgg aagatatcgg ctggaaagtt
    21421 cgcctgtgtc tgatggatac ccagcgcgcg ctcaacttcc tggtacgcaa ggcgcgttta
    21481 ccgggtgggc aactggagca ggcgcgtgaa atcctgcgag atatcgaatc cctgctgccg
    21541 cataacgaat ccctgttcca gaaggtgaac ttcctgatgc aggcggcgat gggctttatc
    21601 aacatcgagc agaaccgcat catcaaaatc ttctcggtgg tatccgtggt gttcctgcca
    21661 ccgacgctgg tggcttccag ctacgggatg aactttgaat ttatgccaga gttgaagtgg
    21721 agtttcggct acccaggcgc gattatcttt atgatcctcg cgggcctggc accgtatctg
    21781 tactttaagc ggaagaactg gttgtaatat gagtgccgga taaagctatt tttttatccg
    21841 gcttatttct aaaaattgtc ttttttaaag tcatgcggat tgaaaactcg ctctatttta
    21901 ttttcttgtg ttaattgata ctcagtaaaa tcgttaatac caatatgagt gttacgataa
    21961 gggtattcat taataaccat taagtaattt ttcacgatgc aattatcatt gctcttaatt
    22021 gtcgggaaac agtagagatc ttcatacggc ccttcgatat attttttata gtcagtgaag
    22081 ccattaccga tatcaatgac ataccaggta tgccaactac cttctggtag gggatgttga
    22141 tttttaattt cattctgatg cttcagatac caatttattt tatcgctgtc agtccatggc
    22201 agatgatcaa cgaccagaaa gactgaattg ccagcatcag agtatgtata caaaatctgt
    22261 acaggacgca atgtccataa attatagatg cagcatagaa gaaataaaca taatataatt
    22321 ttctggagtt tgcttttcat agataatttt cgattctttc gatagtgtta aaattggtga
    22381 aaaaaggttt gaaagcaaag tctttatgcc gttgtaaaaa gaaccatatg cgaaaaaatc
    22441 tatatttgct gtaagtcttg tttttgatat cagttacatc aagaccgaag tggtcctgtg
    22501 ctacaaaatg aattgttgca ctccatccta tagcgtagtt ttgaaaactt aaaagagata
    22561 ttttttgagc tgaaatatca tgaacactga ttcctaagcc attaaaactg tcctcgaagt
    22621 cattaaattt atttagtctt gatgaaagta atttggtttt aatgctttgc aggataagag
    22681 gttggtatcc tgtcgaggga gttgaattaa tgccattttc gatacatt
//
LOCUS       DAZGSP010000079        22669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZGSP010000079 DAZGSP010000000
VERSION     DAZGSP010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.079"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..554)
                     /locus_tag="ACHK74_003446"
     CDS             complement(<1..554)
                     /locus_tag="ACHK74_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP0222200.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWV"
     gene            complement(757..1158)
                     /gene="nikR"
                     /locus_tag="ACHK74_003447"
     CDS             complement(757..1158)
                     /gene="nikR"
                     /locus_tag="ACHK74_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFP0222201.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(1164..1970)
                     /gene="nikE"
                     /locus_tag="ACHK74_003448"
     CDS             complement(1164..1970)
                     /gene="nikE"
                     /locus_tag="ACHK74_003448"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFP0222202.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(1967..2731)
                     /gene="nikD"
                     /locus_tag="ACHK74_003449"
     CDS             complement(1967..2731)
                     /gene="nikD"
                     /locus_tag="ACHK74_003449"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFP0222203.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2731..3564)
                     /gene="nikC"
                     /locus_tag="ACHK74_003450"
     CDS             complement(2731..3564)
                     /gene="nikC"
                     /locus_tag="ACHK74_003450"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFP0222204.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMTATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3561..4505)
                     /gene="nikB"
                     /locus_tag="ACHK74_003451"
     CDS             complement(3561..4505)
                     /gene="nikB"
                     /locus_tag="ACHK74_003451"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFP0222205.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4505..6079)
                     /gene="nikA"
                     /locus_tag="ACHK74_003452"
     CDS             complement(4505..6079)
                     /gene="nikA"
                     /locus_tag="ACHK74_003452"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0222206.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(6190..6777)
                     /gene="acpT"
                     /locus_tag="ACHK74_003453"
     CDS             complement(6190..6777)
                     /gene="acpT"
                     /locus_tag="ACHK74_003453"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFP0222207.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6832..7881)
                     /locus_tag="ACHK74_003454"
     CDS             complement(6832..7881)
                     /locus_tag="ACHK74_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP0222208.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            8013..9230
                     /locus_tag="ACHK74_003455"
     CDS             8013..9230
                     /locus_tag="ACHK74_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0222209.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     SVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(9234..9791)
                     /gene="dcrB"
                     /locus_tag="ACHK74_003456"
     CDS             complement(9234..9791)
                     /gene="dcrB"
                     /locus_tag="ACHK74_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFP0222210.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9864..10529)
                     /locus_tag="ACHK74_003457"
     CDS             complement(9864..10529)
                     /locus_tag="ACHK74_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFP0222211.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10750..10995
                     /gene="tusA"
                     /locus_tag="ACHK74_003458"
     CDS             10750..10995
                     /gene="tusA"
                     /locus_tag="ACHK74_003458"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFP0222212.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(11097..13295)
                     /gene="zntA"
                     /locus_tag="ACHK74_003459"
     CDS             complement(11097..13295)
                     /gene="zntA"
                     /locus_tag="ACHK74_003459"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFP0222213.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTLVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALTIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEEKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13369..13995)
                     /locus_tag="ACHK74_003460"
     CDS             complement(13369..13995)
                     /locus_tag="ACHK74_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFP0222214.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            14136..14495
                     /locus_tag="ACHK74_003461"
     CDS             14136..14495
                     /locus_tag="ACHK74_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFP0222215.1"
                     /translation="MSKPPLFFIVIICLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14498..14767)
                     /locus_tag="ACHK74_003462"
     CDS             complement(14498..14767)
                     /locus_tag="ACHK74_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFP0222216.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14757..15353)
                     /gene="rsmD"
                     /locus_tag="ACHK74_003463"
     CDS             complement(14757..15353)
                     /gene="rsmD"
                     /locus_tag="ACHK74_003463"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFP0222217.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15503..16996
                     /gene="ftsY"
                     /locus_tag="ACHK74_003464"
     CDS             15503..16996
                     /gene="ftsY"
                     /locus_tag="ACHK74_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFP0222218.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNVDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16999..17667
                     /gene="ftsE"
                     /locus_tag="ACHK74_003465"
     CDS             16999..17667
                     /gene="ftsE"
                     /locus_tag="ACHK74_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFP0222219.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17660..18718
                     /gene="ftsX"
                     /locus_tag="ACHK74_003466"
     CDS             17660..18718
                     /gene="ftsX"
                     /locus_tag="ACHK74_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFP0222220.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18963..19817
                     /gene="rpoH"
                     /locus_tag="ACHK74_003467"
     CDS             18963..19817
                     /gene="rpoH"
                     /locus_tag="ACHK74_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFP0222221.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            20088..21191
                     /gene="livJ"
                     /locus_tag="ACHK74_003468"
     CDS             20088..21191
                     /gene="livJ"
                     /locus_tag="ACHK74_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFP0222222.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            21341..21607
                     /gene="ataR"
                     /locus_tag="ACHK74_003469"
     CDS             21341..21607
                     /gene="ataR"
                     /locus_tag="ACHK74_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin AtaR"
                     /protein_id="HFP0222223.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            21614..22141
                     /gene="ataT"
                     /locus_tag="ACHK74_003470"
     CDS             21614..22141
                     /gene="ataT"
                     /locus_tag="ACHK74_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     acetyltransferase AtaT"
                     /protein_id="HFP0222224.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVMGYYTLSGSCFERVALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(22138..22521)
                     /gene="panM"
                     /locus_tag="ACHK74_003471"
     CDS             complement(22138..22521)
                     /gene="panM"
                     /locus_tag="ACHK74_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFP0222225.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSTLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTQGVLDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGL
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         5406 a         5951 c         6267 g         5045 t
ORIGIN      
        1 acccaccacg gcccctgcgg actgtttgtc gccagataac gatgcggact caggcgaagc
       61 tcttccggca gcgcctgcca gagtgcggcc agccggtgac cttcatccag tttcgccaca
      121 ccgccgcgca ccagccagag tgattcgctg cggctgtaac cgtcctcacc gggaaacagg
      181 tgctcaaaat acaggcttct gccgccgtta tcactgagta tcagtgtgtt atcgcgcagc
      241 tgtaagcgga tatccgcagg cattttccag ccggggccga ggctccccac cggcgcgggc
      301 gtttttgtcc ggtaactgct gtaggtgcgg gagagaatga acggcagcgg gccgggcagg
      361 gcgatgtcgg tttcaccggg aaggaccttt gcacccagca gcggattgac cggatggccg
      421 gacgtcaccc cgccggggca caccgaacag gccacgccgg tgggggcacc aatacgtacc
      481 ccggctgaac cctgaacaat gctaccgcca tactgcgtca tgtcaccctg acgcgccgcc
      541 ggttttccgc tcatcgtaaa atccttttct attattttaa tgacagtatc ttcatacgaa
      601 aaagcgaaga aaaacttgtg gtcacaatga attgaagatt tttaggaata tatttagaac
      661 gttacaaaag agaaggttcg gatagggtat ttagagagga atcaggtgtg ctagtggccc
      721 tttgttgatc gttcaccaaa ggacccaata atagactcaa tcttccttcg gcaagcactg
      781 caaatgcccg tgccgcacgc cgcgctgggc gataacgtca tcggcaaaat gctgcacgtc
      841 gcccatatca cctttcagca cggcgatttc cagacagtcg tcgtgattga tatgcacatg
      901 cagcgtggcg acggagagat cgtggtgatg gtgctgggtg gagacaatgc ggctggctaa
      961 gtcgcgtttt tcgtgttcat acacatagga gagcaccgcg aaaccttgcg tgccgtgctg
     1021 ctgagtggcc tcctgggcca gagcgctacg cagaatatcg cggatagctt cggaacgatt
     1081 gttataacca cgacgctggc tcaggctgtc cagcgtctcc agtaaatcgt catcaagcgt
     1141 gatggtgact cgttgcattt gcgttaaacc ttttctgtgg tgcgacggcg cacggggaat
     1201 gcgggtaata ccgcgttttg tagcacacgt ccggcgtcag aggaaaaggt taatttatct
     1261 cccaccacct gggtttcgac gatttgtccg ttgtccatca ccatcacccg ctgacagaag
     1321 cgttccacca ggcgtaagtc gtgggtgatg aacaggcagg cggtgccaaa ctgttgttgt
     1381 agctttttca gcaggcgaat gacgcccgcc tgtaacacga gatcaaggtt agaaacggct
     1441 tcatccagaa tcagtagttt cggttcgacc gccagcgcgc gagccaggca gacgcgctgg
     1501 agctggccgc cgcttaactg cggtgggcgt ttgtcgagaa cgctgtcatc gagatcgacc
     1561 gccttcagca tttcgctggc gcgcgccagt tgttcggatt ttttcagtga tagcaggtgg
     1621 cgcatcggtt cacgcaggat ctcgcgcacg gttttgcgcg gattcacggc gctgatggag
     1681 tcctgaaata ccatctgaat atcgcggcgg aacgctttac gctgggcgcg attgagtttc
     1741 gccagcggtt cgccgcgcca gctaatattc ccttgtgagg gagactctaa gcctaccagc
     1801 agccgcgcga gggtgctttt tccgcagccg ctgcgtccca gcagggcgac ggtttcgccg
     1861 cttttcaggg tcagggaaac gttattcagc accgcctgat gttgatgttt tccgctaaat
     1921 ccaccgtggg catagtgatg ggaaaggccg cagacgttaa gtaaggtcat gatgccagct
     1981 ccataccgta gagggcgaga tgagcggaaa ccaggctgcg cgtcaccgta tgtttggggg
     2041 cgttaaacag cgtttctaca tcgccctgtt cgacaatttt accttgtgac atcaccgcca
     2101 cgtcatccgc cagacgcgcc acaacgccca tatcatgggt gaccagcagc attcccggcg
     2161 cttgtttttg cataatgctt tccagcagat cgaggatgcg cgcctgtgct accacgtcga
     2221 ggtcggtggt cggttcatcg gcgatgataa acggtgattc acacagcacc gccatcgcaa
     2281 tcatcatgcg ctgcaacatg ccgccgctca tctcgaacgg gtacagcttc agcacgcgcg
     2341 cggcgttttc cagccccacc gcttctatgg cagcggtaag cgtagcgtca tcggcgggtt
     2401 tccctaacgc caggcaggtt tcccgcgcgt gggtgtgcat ggtgtgcagt ggattaaagg
     2461 cgctgcgcgg gttctgcatg atggtggcaa ttttgatgcc gcgcagggcg caaggcgaaa
     2521 ccggtttgcc atcggctaaa atttcccccg ccgtctggcg aacgccagcg ggcaaaatgc
     2581 ccagcgtcgc ggcgcaggtt aatgatttcc cgctgccgct accgccgact aacgccagca
     2641 cgcgcccgcg ttgcagggtt aacgatacac cgtgtaccag cggctgcgcg gcctgtagcg
     2701 cgatattacg tagttcaatc tgttgcggca ttagtgtgcg tgctccgtca ccagatgagg
     2761 gtccagatga tcgcgcagtg cgtcacccac caggttaaag gccatcacgc tgataaacag
     2821 cgccagcccc ggccagaaca tttgcagcgg ctgggtccag atatactggc gcgcgtcgtt
     2881 aatcatcacg ccccattcgg cggtcggcgc ggtcacaccg aggccaagga aagacatccc
     2941 cgcgacgtgc agcatcatat ggccgatatc cagcgttgcc agcaccagca gcgaagggat
     3001 caccgcgcct gccagatgat cgacaaacac ccgcacatgg cccgcaccgg aaagccgtga
     3061 cgccagcaca aactcgcgtt ggcgtagtga aatcaccagg ctgcgcacca tgcgtgcata
     3121 ccacgcccag tgcgacaggg cgatggcgat aattacgttg gtcagcccgg tgccgagcac
     3181 gccaaccata aagaacgaca gaatcgaggt cgggaaggtc ataaacatat cggcgacgcg
     3241 catggtggcc tgatcaacgc gcccgccaat caacccggcg ctgccgccaa taaccagccc
     3301 taatgtcagc accagcagca ggcaggccat taccgaaccg agcgacacgc gggtcgctgt
     3361 catcagccgc gagaaaatat cgcgacctaa gtgatcggtg cccagccagt gctgcgcatc
     3421 cggcgaaagc aggcgcgacg gcaaatcaat cgcctggggg tcatacggca gccaccactg
     3481 gctggtgagc gcaatcagcg ccagcagggc gataatgatc agcgccaggc gtaccgacca
     3541 gcgggaagag aggaaaaagt tcacgcgtgc gctccttcat gacgacgaat gcgcgggtcc
     3601 agcgcggcgt tgagcaaatc gacaatcaaa ttacagacca caaaaaccac caccatcatc
     3661 agcgtaaagc actgaatcac cggatagtca cggttaaaaa tcgccgacac cgcatagcgc
     3721 ccgacgcccg gccaggcaaa gatgttttcg ataatcatcg tcccgccgat cagttcgccg
     3781 atgtgcatcc ccacggcggt gatcatcggc agcgaggcat tacgcagaat atggcggcgt
     3841 tcggtctgtt tgtcgttcag gccgcgcaga cgcgcccagg tgacgtgacg ctgaccggcg
     3901 acgtccagca tactggcgcg cagtaaacgc gcgttaatcg ccagcgacat aaaggcaatg
     3961 gaaaccgcag gcaaaatgat gtgctgccag ccgccgtaac ccatcgcggg tagccattgc
     4021 agatacaccg aaaacgccat caccagtaaa aacgccagcc agaagttagg catcgacacg
     4081 ccaagaaacg cgataaagcg cacggcgaaa tccggcaaac gatcgcggtg gcgcgccgcc
     4141 cagataccga gcggtacgga agtgagcaga attaatacca gcgccgcacc tgcaagttcc
     4201 agcgtggcgg gcaggaagtt cagcatatcg tccagtaccg ggcgttggct ggcgaatgag
     4261 ataccaaagt caagatgcag cgccttccac aaccaggtac cgtactggac gtacagcggc
     4321 tgatccagcc cgagcatggt gcgggtagag gccagcatct ccggcgtcgg cggcaggtta
     4381 gataaacgca gataatcgag cgccgggtcg ccggtaccga ggcgcagcat cagaaaaatg
     4441 atcaccgagg cggcgagcac catcgggatc agcagcagaa agcggcgtaa tacgtaacgc
     4501 aacattaagg tttcaccggt ttaatctgtt cgaacggaat ttcggtggcg atcggcgcgt
     4561 aggggatatt acccagctcc ggttttgata ccaccatcat cgagatgtag ctgataggca
     4621 gataaaccgc ctcgtcatgc agacgggtca gaatgtcgcg atacagcgcc tgacgttgcg
     4681 tttcgtcatg ggtcgccagc acttcgccga tctctttatc aatcagcggc ttatcggcta
     4741 atccttgctg tgcctggaag tcagcgtgcg acggtacgcg cattgaactc aggaaggcgt
     4801 gtggatcgta tggcgcgccc caggtgcggt ggaaaatcat gccaaaacga ccgtcgcgct
     4861 gacgagcata gatactgctc tcttcttcgc caatcagcga gacatctgcg ccaatctggc
     4921 gcatatcagc ctgaatgatt tccgccatcg atttgcttaa cgcatcggtg ccgatgaacg
     4981 acagttcaat gcgcagcggc tgaccatttt tctcgcggat gtctttgccc gcaggcagcg
     5041 tccaaccggc tttttccagc aacgctttcg ctttctgcgg atcgtactgg cgcggtttca
     5101 ggccgagatt agcgtaaggc acagaagggg caaacagggt gtcggcgacc tgctgggtgc
     5161 catataacgc gttatcaatc agcgattttt tgtttaccgc gtaattaaga gcttcacgta
     5221 ctgccagctc gttggtgggg gctttggcgg tattgagcgc cagcatcacg gtttcgatcg
     5281 gctgtgacag ttgggtgtgg taagccggat tctggctaaa gcgggcgaag gtatcgagcg
     5341 gtaataaccc ttcgtttccg tacagcaggt cgatatcgcc agtttcaaac gccaccgcgc
     5401 gggtagtcgg gtccgggatg acgttaaagg tgatcttttt aatcgctggc ttttcgcccc
     5461 agtagttttc gttacggacg aagacatcgt actgattcag tttcgattcc tgcaaaatcc
     5521 atggtccggt gccaatcggc gctttaattc cgttcatggt ttcatggttt ttaaactgcg
     5581 agggagcgat aaaacggaaa ggacggggca gggccagttc ttgcaggaaa ggatagtagg
     5641 cgcttttcag ggtaatttgc agctctgttt tactgagtgc tttaacatca acaatctggt
     5701 ttgccagctc cagccaggcg tgacgttgac ggttatcgag cactgcgcgg aagttttctg
     5761 ccgccgcctc ggcatcgaac ggttcaccgt tggagaattt cacgtcatca cgcagggtga
     5821 aggtccaggt tttaccatct tctgaatgag tccagctttt tgccagccac gggatcaccg
     5881 aaccgtctgc ctgatatttc accaatggtt cataaaccat gctctgggcg aacatctggt
     5941 taggcgtgta aaggtgcggg tttagtggcc cgacattcac cggccaggcg gtagtgattt
     6001 catctggtgc agcggcatgg acgataaaag acgcacaagc cagcagcgca aatagagtgc
     6061 ggcggagtgt ggagagcatg ataaccccaa tggattaaaa tagatggcgg aaataagtat
     6121 gacgatttta aatattcgtc atactgatta cctgttctgg atcaatagat gggcagtcgg
     6181 gtgggcggat cagttaactg aatcgatcca ttgcacactg tcggcggtga gcgtaaaggg
     6241 ggtgggggtg cagatcgcca ggcttaaatt ttcaagctga caatggctga ctgacagcga
     6301 ggagtgatag gtgctgtcta cgctgacgat ttgccaggcg ctgccgccac gctgtttaac
     6361 gatggcttct ttgcgcgtcc agatgcgcca gaacatttcc agttgctgat caggatgcac
     6421 ggcgtccatc tcagcgtgtt ccccgaggct gaatacggcg ttcgccagcc agcgccagtt
     6481 ggcgcgcggg cgaatcactt cgatatcgca gccgacttcg ccttcatcac tcaacagcag
     6541 ggcgatatcg tcaccgctat ggcttaagtt gaaccatagc ggcatttccg gcgcaaatgc
     6601 aggtttgcct tgttcgccat agatgatctc cggtagcggg gaaagcgtgt gcgaaagcaa
     6661 tgcacgcccc gccagccagc gttcgcgtcg tggaccttgc ggtgcttgct cgcgtaaacc
     6721 cggtggcagt ggagctgcgc ttaaggtcga aactttcccc agaactatcc gatacatatc
     6781 agggccaacg tttaatggaa aatgaaagtg cgtatcgtat cacttgtcgc ctcatcccgg
     6841 taaccgactt ttcggtctgc ctggccccag taaaatcgcc agtttgctac cgcctttggt
     6901 ggtttccatc cagattttac acacgctggt taatggcacc gaaagtagca tccctaccgg
     6961 gccgagcagc catccccaga ttaacaatga aagaaatacc accatggtgg acatccccag
     7021 gcgatggccc atcatccgtg gctctaaaat attgccgatg accatatgga caactaaaaa
     7081 caatgcgccg accagaatac attcgtaaat accattaaac agcagcacct gaatcattgg
     7141 cggtacggcg gaaattaccg cgccgatatt gggcacgtag ttaagcaaaa acgccagtac
     7201 tgcccacatc agcgcaaact gtacccccat cagctccagc cccagccaga cgatgacacc
     7261 tgtccataaa ctgagtagcg tcttcaatgc aagatagtgc gaaacgcctt taagtgcgcg
     7321 gtgtaatccc gcgatgtgaa tctgtggatt attcagcgca aaacgcattt tgtaagggac
     7381 gtggcgcact tcaaacagca taaaaactac ggtcatcacc agcaaaagca cgctcgccat
     7441 tgccccggaa agcccggtca ttagcgctgt ggtgaaggta accacttttt ccgagtccat
     7501 ccgctgcagc attcgctccg gcgacatatg caaattaaga aaaggcaaca tctcctgcaa
     7561 tttaaaaagt ttgcgcgtca gctccttatt aaacttcggc agcatagaga taaattcgtt
     7621 aaacgatgcc gccagtacgc cgaccagcgc ggttagtgcg atcagcatca ccaccactac
     7681 aatcgtaatg gcaacggggc gttgtactcc ccgacgaata aaccaggtga cgagcgggtt
     7741 gaggacgatg gcaaaaaaca gcgccagtaa cagctgcaca atgatatctg ccgctgcgtg
     7801 aatgcccgcg aggatcacta ccagcgaggc cagcttgagc agaatgtgca tgcccgtttt
     7861 atcgggttga ggggtttcca ttggggcttc cttgtgactt tttgtattaa gtgtagcggg
     7921 agtcacgcca gcaatattct gattcttact gctgattttc acgtcagcac atggtaaaaa
     7981 tgaaacactg ttgtaaaaat gtggtgatcc tcatgcccga acccgtagcc gaacccgcgc
     8041 taaacggatt gcgcctgaat ttgcgcattg tctccattgt catgtttaac ttcgccagct
     8101 acctcaccat cgggttgccg ctcgctgtat taccgggcta tgtccatgat gtgatgggct
     8161 ttagcgcttt ctgggcagga ttggttatca gcctgcaata tttcgccacc ttgctgagcc
     8221 gccctcatgc cggacgttat gccgatttgc tgggacccaa aaagattgtc gtcttcggtt
     8281 tatgcggctg ctttttgagc ggtctggggt atctgacggc aggattaacc gccagtctgc
     8341 ctgtcatcag cctgttatta ctttgcctgg ggcgcgtcat ccttgggatt gggcaaagtt
     8401 ttgccggaac gggatcgacc ctgtggggcg ttggcgtggt tggctcgctg catatcgggc
     8461 gggtgatttc gtggaacggc attgtcactt acggggcgat ggcgatgggt gcgccgttag
     8521 gcgtcgtgtt ttatcactgg ggcggcttgc aggcgttagc gttaatcatt atgggcgtgg
     8581 cgctggtggc cattttgttg gcgatcccgc gtccgacggt aaaagccagt aaaggcaaac
     8641 cgctgccgtt tcgcgcggtg cttgggagcg tctggctgta cggtatggcg ctggcactgg
     8701 cttccgccgg atttggcgtc atcgccacct ttatcacgct gttttatgac gctaaaggtt
     8761 gggacggtgc ggctttcgcg ctgacgctgt ttagctgtgc gtttgtcggt acgcgtttgt
     8821 tattccctaa cggcattaac cgtatcggtg gcttaaacgt agcgatgatt tgctttagcg
     8881 ttgagataat cggcctgcta ctggttggcg tggcgactat gccgtggatg gcgaaaatcg
     8941 gcgtcttact ggcgggggcc gggttttcgc tggtgttccc ggcattgggt gtagtggcgg
     9001 taaaagcggt tccgcagcaa aatcaggggg cggcgctggc aacttacacc gtatttatgg
     9061 atttatcgct tggcgtgact ggaccactgg ctgggctggt gatgagctgg gcgggcgtac
     9121 cggtgattta tctggcggcg gcgggactgg tcgcaatcgc gttattactg acgtggcgat
     9181 taaaaaaacg gcctccggaa cacgtccctg aggccgcctc atcatcttaa aacttactga
     9241 ataaccagcg tattaatgat gttttctgcg gtggtctgcg ctttttgctg atcgtcagcg
     9301 ggcagcgtaa tttgcatggt cagcagttga ttacccacgt tacccagaat aacggaagag
     9361 tacgccgtct ggcctttcgc ggagataata ctgtctaact gctgcatttt gtgacctttc
     9421 agctcaatgg ctttattggt taccacttgc agctgcggat cgcggctacg ttgctgatct
     9481 tccagacgct tcgccagcac cgccagatct tctttcggat catcgcccat gatgacgatg
     9541 actgctttct gcccggtggc gtcggaccag acatgcatgt tattggcctg cgttcccagc
     9601 ttaccgctct ggtcggtcat atccgctggc agcgagaaac ttaacttgcc atcaagcagg
     9661 ttgacgggat tcccggtagc gttactttcc gcgaccgaac cctgcgccgt agcgttagtg
     9721 tctttatcat cacaggccgc aagccccata accagcaggc caattccgac atatttaacc
     9781 agattgcgca ttgacttctt cctttcgata aacggccata acggctcatt catccatctt
     9841 atcacaactc tgataacgaa cctttaactc gcctgcaaag cgttgatttc ggatttatct
     9901 gccagtcttt tcaacagcat attgagtaat acgccataca ttggcaggaa gaaaacgata
     9961 ctgattaaca ctttgaaaca gtaatcgacc agcgcgattt ccatccagtg ttcagccata
    10021 aaggcatccg ggctacgcca gaaggcaatg aagaaaaagg ccagcgtgtc actgacgtta
    10081 ccgaacagtg tggacgctgt cggtgccagc caccagcggc gactctgacg caggcggtta
    10141 aaaacgtgca cgtcgaggat ctgtcccagc gcgtaggcca tgaaactggc ggtggcgata
    10201 cgggcgacaa acaggttgaa gtgggcgagt gcgccgaatc cctgccagga acccatatag
    10261 aatagcgacg agatgacgta ggagattaat aacgcaggga tcattaccgc gaagataatg
    10321 cgtcgggcca gcggtgcgcc aaaaatacgc acggtcaggt cggtagcaag aaaaataaac
    10381 ggaaagctaa acgcgcccca ggtggtatgg aaaccaaaaa tggagacggg aagctgaacc
    10441 agatagttac tggaggtgat caccagcaga tgaaataacg ataaccagaa caacgcctta
    10501 tagcgttgag tttgcgagaa aacgttcata ttgtaccttt ttgattaacc attggggtga
    10561 gggaacccaa tacgtacgac acgtctgtta ccttaacata ataacgtgct tacccttttt
    10621 tcgagccgcc gcatgatact gctttacgtt gacaatgcaa tggttaattt tcacgcaatc
    10681 gttaacctgg tttgcttacg gactcacagg gcgtaaacta gcgccgtttt tttatgtgat
    10741 gagaagaaaa tgaccgatct cttttccagc cctgaccaca cactcgacgc acttggcctg
    10801 cgctgcccgg aacccgtaat gatggtgcgc aaaactgtgc gcaatatgca gcccggcgaa
    10861 acgttgctga ttatcgccga cgatccggcc actacccgcg atattcctgg gttttgtacc
    10921 tttatggaac acgaactggt tgcgaaagag acggatggac tgccttatcg ttatttgatt
    10981 cgtaaaggcg gttgatgggg gctgattggc ttcgatgccg ccttttcccc tcaccctaac
    11041 cctctcccca gaggggcgag gggaccgatc gcgctcaatg ttgcgatcgg tttgccttat
    11101 ctcctgcgca acaatcttaa cgcattcgct gtcaccagca ccgtcgcacc cgtatctgcc
    11161 agcaccgcca gccatagtcc ggtcatccct aacagcgtgg tgacgaggaa gatccctttc
    11221 agtcccagcg caatggtgat gttctggcgg atattggcgt gagtggcgcg tgccagttca
    11281 atcatttgca ccaggccgcg caggtggtta tgggttaatg ctgcatcggc ggtttccagc
    11341 gccacgtctg tgccgctgcc cattgcaatc ccaatggcgg cagctttcat cgctggcgcg
    11401 tcgttaatac catcaccgac catcgccagc ggcgcatgtt gattcagctt ggtcaccgct
    11461 ttgactttct cttccggcaa taggcccgct ttaaactcca gccccagctc cccggcgatt
    11521 gccgccgctg cgcgtggatt atcgccggtg aggatcactc ctttaactcc cagcgcgttc
    11581 agttcactga tggcagttgc agcgtcggcg cgcagggtat cctgcaatgc aatgataccc
    11641 agcacgtcat cattacgtac taccagcaca accgtttgcc cggcgctttc cagttcatta
    11701 atcaaaccag caaatgcatc agcgggatgt ttcccggcag cgcaaatcaa tacgcgctca
    11761 ccgttaacct gcgcttcaat gccagacccg accagcgccc gctgtgactc ggcggcggga
    11821 atggtcagtg cagcaacctg tgcttctcgc acgatggcct gcgccagtgg atgcgtcgcg
    11881 ccttgctcga ccgccgccgc cagtgtcagc agttcagatt cactaatacc cgttgccgga
    11941 tgaatcgcgg taacgcgcgg tttaccgacg gtcagcgtac cggttttatc aaacgccacc
    12001 agggtaacac gacccagttg ttccagcgcc gctccgcctt taattaacgc cccacgacgc
    12061 gctgctgctg ccagcccgga ggtaatcgcc gcgggcgtgg agataactaa cgcgcacggg
    12121 cagccaatca gcagcagcgt caggccttta taaatccact cctgccagct ggcggcaaac
    12181 agcagcggcg gcaccagcgt taccagcagg gcgacggcca taatcgctgg cgtataaata
    12241 cggctgaaac ggtcgataaa ccgctcaatg ggggcgcggc gctcttcggc ttcttcgatc
    12301 agtttcagaa tccggtcaat ggcgctggca cccggttctg acagcacttc cagcgttacc
    12361 agacggtcta cgctggtggc tccagcagga actttatcgc ccgtcgcgcg ctccaccgga
    12421 atggattctc cggtcagggc gctttcatca aaactggcaa acggtgagag cagtttaccg
    12481 tcggcaggca aacgcccacc tgcggcgact tcaatcacat cgccaggccg caggctgtta
    12541 atcgccacct cttcccgctc accgttacgc aggcgcgtgg cggtttctgg tttcagcgcc
    12601 attaacgcgc taacgccctg acgcgcgcgg ctggcggccc agccttccag tcgttcacca
    12661 atcaaaaaca gcagcaacac catcgcagct tcagccgttg cgccaataaa cagtgcacca
    12721 atagcggcta cgctcattaa ggtttcaatg gcgaagtagc tgccggattt gatcagccgt
    12781 aatgcctgac gagcaatcgg gtacagccca accagcgtgg tcgcgataaa cgccagttgc
    12841 ccgaacggat gattgaactg ctccagaccc cagctgattg ccatcatcac gattagtgta
    12901 atcagcggca gattctcttt caggcgcgat gcttgtggct cgtcggcggc ctgctcatcg
    12961 cgcagggaat agcccgcttt ttgcaccgca gattcaactt gtgcacgaat gtcattgtcg
    13021 gcatcaacca ccagtttttc ggtggcgaac agtacctgca cctgattcac acctgcaagc
    13081 tggcgcacgg cattttctac cttgcgcgca caggcggcgc agtccatgcc gctgactttc
    13141 cagctatagc gggtgccgga gacgttttca gagagcgttg gcgagctgga gcatgcgccg
    13201 tcgcagcaac agtcgttggc gttctgtacc gtggttagcg gtttgaacgc agcaaattga
    13261 ggggctttct tgccgtgatt gtcaggtgtc gacatggcat cctccggtta agttttttct
    13321 cattaaccga aggatacact ctggagtcga ctccagagtc aagttttatc agagatacag
    13381 tgagcggacg atgaggaagt gaccggcaaa gtagcaggcc gcagcaatcg cgttatccgc
    13441 acggaagcgt cggcgatagt ggctccccag ccagacaaag ttactgataa acagcaacga
    13501 agcgccaaca aacgcagaga gcgccggagc ggtcggacgg aagaaccaca gttcacctgc
    13561 cagccacacc atcaccagcg tcatgccgat aaacgtgcag ataggccaac gatactcttc
    13621 cagccgcgtc cagataatcg ccagtaacag cgcacccaga accagcaaca ccagtggcag
    13681 aggccagaag aaagagagcg tcatctgact ggcgaaatag atggtgtaca gcaggtgcga
    13741 gaggaaaaac gcgccgatgg cgtacatcag acgttgacgt ggcaacaggg ttagcgcatc
    13801 gcccagcagt gaggcgcaca gccctgccag caccagatag ctaatggcgt cgaacatcgg
    13861 cgcttgccag gccagtaaca gcaggagaag aagggttaac ggtttaaaca cccagcgttg
    13921 ccaggttggc ccacgatacg atgcatccac agatagccat gcggaaagac agacagcgat
    13981 aaacgaccaa agcatcttag ttccttgaat tcgttatttt ttcgtaagcg gaatgcctac
    14041 ggtcttgctt ccagtttagt ggcgagggtg gacggatgac aacaccataa ctggcagggt
    14101 atgcttattt ccgcattttc cgatgaggga taaagatgag caaaccacct cttttcttta
    14161 ttgttatcat ttgcttaatt gtcgtcgccg catcgtttcg ttttatgcaa cagcgacggg
    14221 aaaaagctga taatgatatg gctccgctcc agcaaaagct ggtggtggtg agcaacaagc
    14281 gggaaaaacc gattaacgat cgccgttcgc gccagcagga agtgactccg gcaggcacca
    14341 gtatgcgcta tgaggcaagc ttcaaaccgc aaagcggagg aatggagcag acgtttcgcc
    14401 tcgacgccca gcagtaccac gccctgacag tgggcgataa aggtacgctg agctataaag
    14461 gaacgcgctt tgtcagcttt gtaggcgaac aataaggtta ttttttaact ttaaatttct
    14521 tctgccagac cagcagttca aacacgccga aaaggaaaat ccgcaccttt tcggcggtgg
    14581 tcatctgcgg gccatctttc ggtaaagtgg atttcaatag cgccagttgc ataccatgca
    14641 tcagcacggt aaaaatcagc gccacgttaa cgaaaatatt cagcgggcgc gggaagggat
    14701 gcaccagatt gaggattaaa aatccccaaa cgcagagcat taacaaacga ccaatattaa
    14761 tcagcatcac tttctccttg tgcttcgcgt tgatacagcc gataagccac ctgacccgcc
    14821 actttttccc gatgtaatga ccagtttgcc ggaacagtgg gcagaccgtt ttcgacttcg
    14881 ctttcgacat aaatcagggc ttcgtcagcc agccagccgt tatcttccag taaatttatc
    14941 gtctcttcta acaagccacg gcggaacggt ggatcgacaa acacgatatt atgcggtgtg
    15001 cctttttgcg ccaggaatga cattgcgttg ctgttcacca cgcgtgcatt gcctgctttt
    15061 agtgtcgcca gattcttaat taactgctga gaaactgcgc gatccatctc aatcaacgtg
    15121 gcccccgcag cgtagcgcga taacgcttcc agccccagcg cgccgctccc ggcgaagcaa
    15181 tccagacatt gggcgtcaac aatgaccgga gccagccagt taaacaacgt ttcgcgtacg
    15241 cggtcggtgg tggggcgcag acctgggcta tcaggaaccg ggagtttacg gcctcgccac
    15301 tgcccgccaa taatgcggat ttggccgctg ccggaatgat tcggtttttt catgataaat
    15361 tgctcaatcc gccagataac acatacttgc aggcggtgat gtgaattaag ttaagtgata
    15421 gactatttca tcattttttt agctgctatg tacatagcgt taacgctgtg ccatgaagca
    15481 acagcgagga gtgtagtcgc aaatggcgaa agaaaaaaaa cgtggctttt tttcctggct
    15541 gggctttggt caaaaagagc agaccccgga aaaagagaca gaagttcaga atgaacaacc
    15601 ggttgtagaa gaaatcgttc aggcgcaaga gcctgtgaag gcttctgaac aagccgtcga
    15661 agagcagccg caggcgcata ctgaagccga ggcggaaact tttgctgccg acgttgtgga
    15721 agtcactgaa caggttgctg aaagtgaaaa agcgcagcct gaagcggaag tcgttgcaca
    15781 gccggaaccg gtcgtagaag aaacgccgga gccagtggct atcgagcgtg aagaactgcc
    15841 gttgccggaa gacgtcaacg ccgaagaggt ttcgccagaa gagtggcagg ctgaagcgga
    15901 aaccgtagag attgtcgaag cggcggaaga agaagcggct aaagaagaaa tcaccgacga
    15961 agagctggaa gcacaggcgc tggctgccga agcggcagaa gaggcggtga tggtggttcc
    16021 tccggtagaa gagcagccgg tggaagaaat cgctcaggag caggaaaaac cgaccaaaga
    16081 aggtttcttc gcgcgcctga aacgcagcct gttaaaaacc aaagaaaatc tcggttccgg
    16141 atttatcagc ctgttccgcg gtaaaaaaat cgacgatgat ctgtttgagg agctggaaga
    16201 acagctattg atcgccgatg tgggcgtgga aaccacacgt aaaattatca ccaatctgac
    16261 ggaaggcgca tcccgcaagc agcttcgtga tgccgaggcg ctctatggcc tgctgaaaga
    16321 agagatgggc gagattctgg cgaaagtcga tgagccgctg aatgttgaag gtaaaacgcc
    16381 gttcgtgatc ctgatggtgg gcgtcaacgg tgtgggtaaa accacgacga ttggtaagct
    16441 ggcgcgtcag tttgagcagc agggtaaatc ggtgatgctg gcggcgggcg atactttccg
    16501 tgcagctgcg gttgaacagc ttcaggtctg gggtcagcgt aacaatatcc cggtgattgc
    16561 ccagcatact ggtgcggatt ccgcttcggt tatcttcgac gccattcagg ctgcgaaggc
    16621 gcgtaatgtt gacgtactga ttgccgatac cgccggacgc ctgcagaaca aatcgcacct
    16681 gatggaagag ttgaagaaaa tcgtccgcgt gatgaagaaa ctcgacgttg aagcgccgca
    16741 tgaagttatg ctgactatcg atgccagcac cgggcagaac gcggtaagcc aggccaaact
    16801 gttccatgaa gctgttggct taaccggcat cacgctaacg aaactggacg gcacggcgaa
    16861 aggcggggta attttctcgg tggctgacca gtttggtatc cctatccgct acattggtgt
    16921 cggcgaacgt attgaggatt tgcgtccgtt taaggcggac gactttatag aggcactttt
    16981 tgcccgagag gattaacaat gattcgcttt gaacatgtca gcaaggctta tctcggtggg
    17041 agacaggcgc tgcagggcgt tacgttccat atgcagccgg gtgagatggc gtttctgacc
    17101 ggtcattccg gcgcagggaa aagtaccctc ctgaagctga tctgtgggat tgagcggccc
    17161 agcgccggga aaatctggtt tagcggccat gacatcacgc gtctgaaaaa ccgtgaagtt
    17221 ccgtttctgc gccgccagat tggcatgatt ttccaggatc accatctact gatggaccgt
    17281 actgtctacg ataacgtggc aatcccgctg attatcgccg gtgccagcgg tgacgatatt
    17341 cgtcgccggg tgtcggcggc actggataaa gtcgggctac tggacaaagc gaagaacttc
    17401 cctattcagc tttctggcgg tgaacaacag cgtgttggca ttgcccgcgc ggtggtgaac
    17461 aagcccgcgg tactgctggc ggacgaaccg actggtaacc tggacgacgc gttgtcggaa
    17521 gggattctac gtctgtttga agagtttaac cgcgttgggg taaccgtatt gatggcaacg
    17581 cacgacatca acctgatctc gcggcgttcc tatcgcatgc tcaccctgag cgatggtcac
    17641 ttgcatggag gcgtgggcca tgaataagcg cgatgcaatc aatcatattc ggcagtttgg
    17701 cgggcgtctg gatcgcttcc gtaaatcggt cggcggctct ggcgacggcg gtcgtaacgc
    17761 gccaaaacgc gcgaaatcct cgccaaaacc ggtaaatcgc aaaaccaacg ttttcaacga
    17821 acaggtgcgc tatgccttcc acggcgcatt gcaggatctg aaaagcaaac cgttcgccac
    17881 gtttttaacg gtgatggtta tcgccatctc cctgacgctg cccagcgtct gttatatggt
    17941 gtacaaaaac gttaaccagg cggcgacgca atattatccg tcaccgcaaa tcactgttta
    18001 cttgcaaaaa acgctggacg atgacgccgc agcaggcgtg gtagcacagc tacaggccga
    18061 gcagggcgtg gagaaagtga actatctttc tcgtgaagac gcactgggcg agttccgtaa
    18121 ctggtctggt tttggtggtg cgctggatat gctggaagaa aacccgcttc cggcagtggc
    18181 ggtggtgatc ccgaaactcg attttcaggg gacggaatca ctgaatacgc tgcgtgatcg
    18241 tatcacccag attaacggca ttgacgaagt gcggatggat gacagctggt ttgcccgtct
    18301 ggcggcgttg accgggctgg tcgggcgcgt ttccgcgatg atcggcgtgt tgatggtggc
    18361 agcagtgttc ctcgtcatcg gcaacagtgt gcgtctgagc atctttgctc gccgtgactc
    18421 cattaacgtg cagaaactga ttggtgcgac ggatggtttc atcctgcgcc cgttcctgta
    18481 cggaggcgca ttgctgggat tttctggcgc attgctgtca ttaattttgt cagaaattct
    18541 ggtactgcgt ttgtcatcgg cggttgcaga agtggcacag gttttcggaa cgaagtttga
    18601 tatcaatggt ttatcgttcg atgaatgcct gctattgctg ctggtatgct cgatgattgg
    18661 ctgggtggca gcgtggcttg ccacggtaca acatttacgc cactttacgc ctgaataata
    18721 aaagcgtgtt atactctttc cctgcaatgg gttccgtagc agggaaagag atcccgttgt
    18781 ctcttcccgg tatttcatct ctatgtcaca ttttgtgcgt aatttattca caagcttgca
    18841 ttgaacttgt ggataaaatc acggtctgat aaaacagtga atgataacct cgttgctctt
    18901 aagctctggc acagttgttg ctaccactga agcgccagaa gatatcgatt gagaggattt
    18961 gaatgactga caaaatgcaa agtttagctt tagccccagt tggcaacctg gattcctaca
    19021 tccgggcagc taacgcgtgg ccgatgttgt cggctgacga ggagcgggcg ctggctgaaa
    19081 agctgcatta ccatggcgat ctggaagcag ctaaaacgct gatcctgtct cacctgcggt
    19141 ttgttgttca tattgctcgt aattatgcgg gctatggcct gccacaggcg gatttgattc
    19201 aggaaggtaa catcggcctg atgaaagcag tgcgccgttt caacccggaa gtgggtgtgc
    19261 gcctggtctc cttcgccgtc cactggatca aagcagagat ccacgaatac gttctgcgta
    19321 actggcgtat cgtcaaagtt gcgaccacca aagcgcagcg caaactgttc ttcaacctgc
    19381 gtaaaaccaa gcagcgtctg ggctggttta accaggatga agtcgaaatg gtggcccgtg
    19441 aactgggcgt aaccagcaaa gacgtacgtg agatggaatc acgtatggcg gcacaagaca
    19501 tgacctttga cctgtcttcc gacgacgatt ccgacagcca gccgatggca ccggtgctct
    19561 atctgcagga taaatcatct aactttgccg acggcatcga agatgataac tgggaagagc
    19621 aggcggcaaa ccgtctgacc gacgcgatgc agggtctgga cgaacgcagc caggacatca
    19681 tccgcgcgcg ctggctggac gaagacaaca agtccacatt gcaggaactg gctgaccgtt
    19741 acggcgtttc cgctgagcgt gtgcgccagc tggaaaagaa tgcgatgaaa aaactgcgcg
    19801 ctgctataga agcgtaattt ccgctattaa gcagagaacc ctggatgcga gtccggggtt
    19861 tttgtttttt gggcctctac aataatcaat ttcccctccg gcaaaacgcc aatccccacg
    19921 cagattgtta ataaactgtc aaaatagcta ttccaatatc ataaaaatcg ggtatgtttt
    19981 agcagagtat gctgctaaag cacgggtagt catgcataaa acgaaataaa gtgctgaaca
    20041 acaacatcac aacacacgta ataaccagaa gaatggggat tctcaggatg aacataaagg
    20101 gtaaagcgtt actggcagga tgtatcgcgc tggcattcag caatatggct ctggcagaag
    20161 atattaaagt cgcggtcgtg ggcgcaatgt ccggtccggt tgcgcagtac ggtgaccagg
    20221 agtttaccgg cgcagagcag gcggttgcgg atatcaacgc taaaggcggc attaaaggca
    20281 acaaactgca aatcgtaaaa tatgacgatg cctgtgaccc gaaacaggcg gttgcggtgg
    20341 cgaacaaagt cgttaacgac ggcattaaat atgtgattgg tcacctctgt tcttcatcaa
    20401 cgcagcctgc gtctgacatc tacgaagacg aaggcatttt aatgatcacc ccagcggcaa
    20461 ccgcgccgga gctgaccgcc cgtggctatc agctgatcct gcgcaccacc ggcctggact
    20521 ccgaccaggg gccgacggcg gcgaaatata ttcttgagaa agtgaaaccg cagcgtattg
    20581 ctatcgttca cgacaaacag caatacggcg aaggtctggc acgagcggtg caggacggcc
    20641 tgaagaaagg caatgctaac gtggtgttct ttgatggtat caccgccggg gaaaaagatt
    20701 tctcaacgct ggtggcgcgt ctgaagaaag agaatatcga cttcgtttac tacggtggtt
    20761 atcacccgga aatggggcaa atcctgcgtc aggcacgcgc ggcggggctg aaaactcagt
    20821 ttatggggcc ggaaggtgtg gctaacgttt cgctgtctaa cattgcgggc gaatcagcgg
    20881 aagggctgct ggtgaccaag ccgaagaact acgatcaggt tccggcaaac aaacccattg
    20941 ttgacgcgat caaagcgaaa aaacaggacc caagcggcgc attcgtttgg accacctacg
    21001 ccgcgctgca atctttgcag gcgggcctga atcagtctga cgatccggct gaaatcgcca
    21061 aatacctgaa agcgaactcc gtggataccg taatgggacc gctgacctgg gatgagaaag
    21121 gcgatctgaa aggctttgag ttcggcgtat ttgactggca cgccaacggc acggcgaccg
    21181 atgcgaagta attgttagat aaatgaaaat gcccggtgcg cttaacgtcc atcgggtttt
    21241 tttgtgtttg tcgaaatggt tgtagaaaga ttaaacaatc caggatatgc ttattgtacg
    21301 gtgatatgcc gtacatatta tacggagcag gagagttcac atgtctgctg ttaaaaagca
    21361 gcgtatcgat ctgcgtttaa ctgacgatga caaaagtatg attgaggaag cggcagcgat
    21421 atctaatcag tccgtcagcc aatttatgtt gaacagcgct tcgcagcggg ctgcggaagt
    21481 gattgaacaa catcggcggg tgatcctcaa tgaggaatcc tggacgcggg tgatggatgc
    21541 gctgagtaat ccaccgtcac caggtgaaaa gctaaaacgt gcggcaaaac gtcttcaggg
    21601 aatgtaataa tttatggatg atctgacgat agagattctg accgatgatg cagattatga
    21661 tctacagcga ttcgactgcg gcgaggaagc gttaaatctc tttctgacga cacatctggt
    21721 tcgtcaacat cgcaataaaa ttctgcgagc gtatatcctt tgtcgcaaca ctccagaacg
    21781 tcaggtcatg ggatattaca cattatccgg cagttgtttt gaacgagtcg cactaccctc
    21841 gaaatcgaaa cagaaaaaaa ttccctacaa aaatattccc agcgtcactc ttgggcgtct
    21901 ggcaattgat cgttcattac aggggcaggg atggggagca acactggttg ctcatgccat
    21961 gaaagtcgtc tggtcagcct ctttagcggt aggtattcac ggtctttttg tcgaggcgct
    22021 gaatgaaaaa gcccatacgt tttatcaatc gctgggcttt atccctttag tcggggaaaa
    22081 cgaaaatgcg ttatttttcc caaccaaatc cattgaactg ctttttacac agagcgatta
    22141 acgcttctcc cagccgccct gttgtgccgt aaaccccagc gcctgcataa acgccgtcat
    22201 cacaccgcga tcttccaagc ctgcatccgc catccaccag catgaaacgc caggattgtt
    22261 acgcaaaacc tcttccagca gatattgccc cacaccgcgt cggcgggtga cttcccgtac
    22321 gcgcaaagaa tccagaaccc cctgagtgcc gcttaaggtt acccgcacgg ccgcgagcag
    22381 gcgctcgtta aaacgcgcgg cgtagatacg gtggttatcg tcaacctgta aagtggaagg
    22441 ggaatactcc ggccagatct tttgcaggtc aatccggtcc tggtcgctaa agttttctaa
    22501 tcgaatgatg gtcagcttca tggggaaccc gtgtaaatca caaaagtgga accagtgtag
    22561 cgaaataatt taatcggagg ctttctcttt tttatttctt ttggcaggtg attaattttt
    22621 taacagcaat aattacaaag ttaaaacatt atataatgaa aaataccca
//
LOCUS       DAZGSP010000080        22621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZGSP010000080 DAZGSP010000000
VERSION     DAZGSP010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.080"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(126..1580)
                     /gene="amn"
                     /locus_tag="ACHK74_003472"
     CDS             complement(126..1580)
                     /gene="amn"
                     /locus_tag="ACHK74_003472"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFP0222226.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            complement(1682..2998)
                     /gene="shiA"
                     /locus_tag="ACHK74_003473"
     CDS             complement(1682..2998)
                     /gene="shiA"
                     /locus_tag="ACHK74_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFP0222227.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            3313..4365
                     /locus_tag="ACHK74_003474"
     CDS             3313..4365
                     /locus_tag="ACHK74_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFP0222228.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPVCKSASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            complement(4627..11703)
                     /gene="yeeJ"
                     /locus_tag="ACHK74_003475"
     CDS             complement(4627..11703)
                     /gene="yeeJ"
                     /locus_tag="ACHK74_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
                     /protein_id="HFP0222229.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLAFPMAAA
                     AQGVVNAATQQPVPAQFAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSENNLTPPPGNSSGNLEQQIASTSQPIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPYLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNLSNREQSMVVVQAPTLSQKDSSVSLSTQTLNADSHSTATLTFIAHDAAGNPV
                     VGLVLSTRHEGVQDITLSEWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISISSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNNAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVELQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAEAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQANQQVIFIGDQSTAALTLSVPSGDITVTNTAPLHMTATLQDKNGNPLI
                     DKEITFSVPNDVASQFSISNGGKGMTDSNGVAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPSNHPVAGITVTFTMPQDV
                     AANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNTSDSQPVTFVADKTSA
                     QVVLQMSKDEITGNGVDNATLTATVKDQFDNEVNNLPVTFSSASSGLTLTPGVSNTNE
                     SGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIELTPVPDSIIAGT
                     PQNSSGSVITATVVDNNGFPVKGVTVNFTSRTNSAEMTNGGQAVTNEQGKATVTYTNT
                     RSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALFDTVSAGDTTNLY
                     IDVKDNYGNGVPQQEVTLRVSPSEGVTSSNNAIYTTNHDGNFYTSFTATKAGVYQVTA
                     TLENGDSMQQTVTYVPNVANAEITLAASKDPVIADNNDLTTLTATVADTEGNAIANTE
                     VTFTLPEDVKANFTLSDGGKAITDAEGKAKVTLKGTKAGAHTVTASMTGGKSEQLVVN
                     FIADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEAVTFTLPADVSASF
                     TLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVTLIADAGTAKLAS
                     LTSVYSFVVSTTEGATMTASVTDANGNPVEGIKVNFRGTSVTLSSTSVETDDRGFAEI
                     LVTSTEVGLKTVSASLADKPTEVISRLLNASADVNSATITSLEIPEGQVMVAQDVAVK
                     AHVNDQFGNPVAHQPVTFSAEPSSQMIISQNTVSTNTQGVAEVTMTPERNGSYMVKAS
                     LANGASLEKQLEAIDEKLTLTASSPLIGVYAPTGATLTATLTSANGTPVEGQVINFSV
                     TPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQTQTTVKVTGNSST
                     AHVASFIADPSTIAATNTDLSTLKTTVEDGSGNLIEGLTVYFALKSGSATLTSLTAVT
                     DQNGIATTSVKGAMTGSVTVSAVTTAGGMQTVDITLVAGPADTSQSVLKSNRSSLKGD
                     YTDSAELHLVLHDISGNPIKVSEGMEFVQSGTNVPYIKISAIDYSLNINGDYKATVTG
                     GGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGA
                     YYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTFKNVGSNWERITATPKSGGPSY
                     VYEIRVKSWWVNAGEAFMIYSLAENFCSSNGYTLPRANYLNHSSSRGIGSLYSEWGDM
                     GHYTTEAGFQSNMYWSSSPANSNEQYVVSLATGDQSVFEKLGFAYATCYKNL"
     gene            complement(12017..12092)
                     /locus_tag="ACHK74_003476"
     tRNA            complement(12017..12092)
                     /locus_tag="ACHK74_003476"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12057..12059),aa:Asn,seq:gtt)
     gene            complement(12193..12990)
                     /gene="mtfA"
                     /locus_tag="ACHK74_003477"
     CDS             complement(12193..12990)
                     /gene="mtfA"
                     /locus_tag="ACHK74_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFP0222230.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
     gene            13084..13173
                     /locus_tag="ACHK74_003478"
     tRNA            13084..13173
                     /locus_tag="ACHK74_003478"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13118..13120,aa:Ser,seq:cga)
     gene            complement(13743..14273)
                     /gene="yodB"
                     /locus_tag="ACHK74_003479"
     CDS             complement(13743..14273)
                     /gene="yodB"
                     /locus_tag="ACHK74_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFP0222231.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(14616..15266)
                     /gene="zinT"
                     /locus_tag="ACHK74_003480"
     CDS             complement(14616..15266)
                     /gene="zinT"
                     /locus_tag="ACHK74_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFP0222232.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(15523..16158)
                     /gene="msrQ"
                     /locus_tag="ACHK74_003481"
     CDS             complement(15523..16158)
                     /gene="msrQ"
                     /locus_tag="ACHK74_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFP0222233.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(16159..17163)
                     /gene="msrP"
                     /locus_tag="ACHK74_003482"
     CDS             complement(16159..17163)
                     /gene="msrP"
                     /locus_tag="ACHK74_003482"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFP0222234.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(17272..17685)
                     /gene="hiuH"
                     /locus_tag="ACHK74_003483"
     CDS             complement(17272..17685)
                     /gene="hiuH"
                     /locus_tag="ACHK74_003483"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFP0222235.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            17818..18489
                     /gene="hprR"
                     /locus_tag="ACHK74_003484"
     CDS             17818..18489
                     /gene="hprR"
                     /locus_tag="ACHK74_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFP0222236.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            18489..19847
                     /gene="hprS"
                     /locus_tag="ACHK74_003485"
     CDS             18489..19847
                     /gene="hprS"
                     /locus_tag="ACHK74_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFP0222237.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(19955..20806)
                     /gene="hchA"
                     /locus_tag="ACHK74_003486"
     CDS             complement(19955..20806)
                     /gene="hchA"
                     /locus_tag="ACHK74_003486"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFP0222238.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDIAGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(21398..22561)
                     /gene="ompC"
                     /locus_tag="ACHK74_003487"
     CDS             complement(21398..22561)
                     /gene="ompC"
                     /locus_tag="ACHK74_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP0222239.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKLDLYGKVDARHTF
                     SDNPGDDGDETIIELGFKGETQITDQLTGYGQALTKTKASDTEGSDNTYVKLAFAGLK
                     FGEMGSFDYGRNYGVIYDVEAWTDMLPVFGGDSYTWTDNFMAGRANGVATYRNSDFFG
                     LVEGLNFALQYQGNNEGSNAGEDQEGTKNGHEDVRFQNGDGFGLSTSYDFDFGLSLGA
                     AYSNSDRTNSQVALGGYHYNEYSKFAGGDTAEAWTFGAKYDANNVYLAMMYAETRNMT
                     PYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYVYSKGKDLGGNDYNNNGHQEYVDQDL
                     VNYVEIGATYYFNKNFSTYVDYKINLLDKDDDFYDNNGIATDDVVGVGLVYQF"
BASE COUNT         5964 a         5119 c         5344 g         6194 t
ORIGIN      
        1 attaagacga taataaaaca cgtatccgcc caaggaagca aacagcccac gattattgca
       61 ggctatttgc caggtgtctt acttactcct tcacatctat ttctgataag aaggttcaga
      121 acttattatc ggaacggcgg ctcattaaag gttcgtaatt tacgcgaatg cagtcggtcg
      181 ccttctgcgc gcagcaaatc gatcgcccga atgccaattt gcagatgttc ggaaatagcg
      241 ccttcataaa aacggttagc ctggccggga agtttaatct cgccatgcaa cggtttatct
      301 gaaacacaca gtagtgtccc gtatggcacg cggaaacgat atccttgcgc ggcaatggtt
      361 gcactttcca tatcaattgc tacggcccgg cttaagttaa aacgaagtgc agaagctgag
      421 taacgtaatt cccagttcct gtcatctgtg gttaccacag taccagtacg tagccgctgt
      481 ttgacttcct caccgggcct gccgctcacc agcttggtgg cgtcataaag cgcacgttgc
      541 acttcagcaa tgctcggaat aggaatatcg ggcggcagaa ccgcatcaag aacgtggtca
      601 tcgcgtaaat aagcgtgtgc aagtacataa tcgccaatgg cctgactttc acgtaatccg
      661 ccacagtgac caatcatcaa ccagacatcc gggcgtagca ctgccagatg atcgcagatg
      721 gttttagcat ttgacggtcc cacgccaata ttcaccagag taataccctg accatcggcg
      781 gtaattaaat gccatgctgg catctgatgt tttttccatg caaggtcgga aatggcttct
      841 tctggcgctt cggtttcggc ggtgatccag ttcccgccag cacaagaaag ggcaatgtag
      901 ggactatcag gatcgaggat ctggctgcat ccccaacgaa cgaattcatc cacataacgt
      961 gtgtagttgg taaacaagac gaacggctga aaatgttcaa ctggcgtacc ggtataatgg
     1021 cgcaaccgcg cgagggaaaa atcgacgcgg cgcgcatcaa aatgcgatag cggggagaat
     1081 tcagttggat gataaatgcc gtctgcagtt tcatcgccaa tttgcgccag ttctgttgtc
     1141 gggaagtagc gagttaaccc agcgctcatt gagcgatcaa gtgtcaattc agagccatcg
     1201 atgacataag gataggggat ttcatgctgc gagggttgca ccgagatatg cgcaccataa
     1261 tcctgataca gcaacgtaag ttgttcattg agatacgaac gaaagagagt agggcgagta
     1321 atcgtggtgg tgtagctgcc tgcgtgagta aaacgaccaa atgcgcgcgt tttgggggga
     1381 ttggttgtgc taccgtccca ggttacggtc agtgatggat agacaaaaag accttgtttg
     1441 cgggcgtttt catcaggtaa ttcgccactt gtaatatagt tgccaatggc gttgcgtaat
     1501 gcgactacag attgctcata cagcgcgtcg agtttatcca gtgcctgagc tggggtcaga
     1561 ccggagccct tattattcat gtgtgtctcc tgttccatac aattaccgca catccgatag
     1621 tatgtcacag gaatgtgaaa caaaagtgta aggcgaagat cggatgatct tcgccagact
     1681 atcaagcgcg ttgattgtct ttcatcaaca aagcggtcat tgcggaaatc agacatccag
     1741 ccagcaaata aatggcgacg ctatgccagt tcccggcaaa gtaagtgatg agtgcagcgg
     1801 caataaaagg tgtaaatcca ccgccaacca cactggcgac ctgataaccg actccagcac
     1861 cactatagcg ataactggca ccaaacattt cggtaaacat cggttgttgc acacacacca
     1921 ccatgtcatg cgcaatgttt gccagcatta tggagaagaa aactatccag aaaatagatt
     1981 gtgcttcaag cgccataaag aaaggaaatg cgctcaacgt tccgattaac gcacctgtga
     2041 tataaaccct gcggcgacca aaacgatcgg caagccaggc aaaacaggga attgtcaagc
     2101 agcttaatcc acctaccagc aaaccaatat tgaggaaaag ttcgcgcggt aaccccatat
     2161 tctgggttga ataattaagt gcaaaggcag taacgatgta catcgtcagc aattcgcaca
     2221 gtcgtagcgc aataatcttc aggaaagcac cgggatgtcg taacagcgct tcgataaccg
     2281 ggatgcgttt tttcgcggca gcttgataat gttgctgttg ttcaaattcc gcggactcct
     2341 ccatgccatt gcgcacccac aatgctccca gtaccagtac gatgctaaac aggaaaggaa
     2401 tgcgccagcc ccagcttaaa aactgttcgt cagtcgtcat catactgatc aatgaaacca
     2461 gtccggttga aagcagtaaa cctacaccgt agccaacttg tacaccgcta ctgtaaaagg
     2521 cttttttatt tttcggtgca ctttcaacgg aaagcaacgc cgcgcctccc cattcgccgc
     2581 cgacggcaaa tccctgaatg gcacgcagtg tcaccagcaa aataggtgcc caccacccaa
     2641 tggtcgagaa tgaaggaaga ataccaatca aggctgtcgc gatgcccatc atccagacgg
     2701 tcagcattaa catgcgctta cgtcccagtc ggtcgccaaa gtgaccgaaa atgacaccgc
     2761 cgagcggacg gaaaaggaat ccgacgccaa aggtggcaaa tgcggcgagc gttcccatcg
     2821 ccgggcttac ttgcgggaaa aactcgcgat taaacaccag tgcggcggtg atgccataga
     2881 gtaaaaaatc ataccagtcg acgacggcac cagcgaagct gcctaacgca gctcgtcggg
     2941 cgcgacttaa cgaaagcgtc ccttcatcgg gacgagtgga gatgagcgtg gagtccataa
     3001 tgatcctgtt tgtactgtct ttttataatt agtatggaaa aggtaactca cattgccgtc
     3061 gatctataaa cgaaccttat gagactacct cgacagagcg catctgcaaa tgtttttatg
     3121 gctgattagc ggcaacatga aaaattcgat ggaagatgaa agcgcaggag ttgctggtgt
     3181 tgtttaagga ttaagcagag gccccacagg ttcgagatat gaaacataaa agtccgcata
     3241 tgcacaaaat ggcaccctga tgagtctgat agactgcatt gcattataac cagtagggag
     3301 gcgaggttag gtatgttttt agcatcattg ttgagacgta ttgcatttag ttactacgat
     3361 tataaagctt ataatttcaa tattgaaaaa acagactttg ttgtcatcca tattcccgat
     3421 cagattggcg atgctatggc catctttcct gttattcggg cgcttgaatt gcataaaatt
     3481 aagcatctat taattgttac gtcgacaatt aatttagaag tctttaatgc gctcaaactt
     3541 gaacagacta aattaacatt agtcacaatg actatgcagg atcacgcaac attaaaagaa
     3601 ataaaagatt tagcgaagaa cataacacag caatacggta cgccggatct ttgtatagag
     3661 gcgatgcgta aaaagaacct gaaaacgatg ttatttatca gtcagttgaa agcaaaaacg
     3721 aattttcagg ttgttggttt aaccatgaag tgctattccc ctgtgtgtaa gagcgcgtcc
     3781 cggatggatc agaatctccg ggctcccgta cccatgacat gggcatttat gatgcgtgag
     3841 gcgggttttc cagcagtcag gccaatatat gaattgccac taagtgagga tgtactcgat
     3901 gaggtacgcg aggaaatgcg atcgttagga tcttacattg cgcttaattt agaaggtagc
     3961 tcgcaggaac gtacattttc actatcgatt gcagaaaatc taatagcaaa aattcaaagt
     4021 gaaacagata taccaatagt gatcgttcat ggaccaaaag gggaagataa agccagggta
     4081 ttagtggatt gttataataa tgtctaccgt ttatccttat caccctcgat taaacgttca
     4141 gcagcaatca taaaagatgc ttatatcgca ataactcctg acacctcaat attacatatg
     4201 gcaagtgcct ataatacccc cgttgttgca atttatgctg attacaaaac gcgatggccc
     4261 gcaatggccg atgtttcgga gtcagtcgtc gttgggcaaa aaattgacaa tataagtctg
     4321 gatgaattcg caaaggcatt aaaaagtgtt ttggcgagaa tatgagacag tacttatgca
     4381 tagtaatgcc gatctgttat gaacggtggt gtcatcaata ctctgaggct ggtcaatcat
     4441 cacagaaaac cagcctttgg atcaaagcag tactcaccgg atacgatcat agtcacaccg
     4501 aacgcgattc ttgcggtgca gcatttttat ttattcattt tctgtgagag aagggcaagc
     4561 tctatagagg gaatgtaaaa aagagtgctc cgaatggagc actctttaag aaattataac
     4621 ggaaaatcag aggtttttat aacatgtcgc ataagcaaac ccaagctttt caaatacgct
     4681 ttgatcacct gttgccaggg aaactacgta ttgttcgttt gagtttgcgg gactagatga
     4741 ccaatacata tttgattgaa aaccagcttc agtcgtgtaa tgccccatat ctccccattc
     4801 actgtacagt gacccgatgc ctcgggaact actgtggttt aaatagtttg ctctggggag
     4861 cgtgtagcca ttgctgctgc aaaaattttc agcaaggctg tatatcatga aagcctcgcc
     4921 ggcgttcacc caccaactct tcacacggat ttcgtataca tagctagggc ctcctgattt
     4981 tggcgtcgcc gtaatccttt cccaattgct gccgacattt ttaaatgtca ctttacctgt
     5041 agcatcaaca tcgacccagg aggcagagct tgaaaactca taatcagccg ccgtttttcc
     5101 tggggcaaag ttgtcattat tcaactgata atacgccccg gtgaacccct gcgaagggaa
     5161 tgtagttgtc ggtaggtcag taccattgac tgatactgta ccgctcatta ttttgtcttc
     5221 tgcgcgagtg aattgtattg tggtactcag accagcttga tgaacaccat tcaatacagg
     5281 gatcagcgtt gcgataccct ctccgccgcc tgtaacagtg gctttgtaat caccgttgat
     5341 atttagactg taatcaattg cgctaatttt tatatagggc acgttagtac ctgattgcac
     5401 aaattccatc ccttcagaaa ctttgatcgg attgcctgat atatcgtgca aaacaagatg
     5461 tagctcagca ctatcggtat agtccccttt cagtgatgac cgattgctct taaggacgga
     5521 ctgcgaggtg tctgccgggc cagccaccag cgttatatct actgtttgca ttccaccagc
     5581 ggtcgtgact gcgcttaccg tgacgctacc tgtcatcgct cctttcacgc ttgttgtcgc
     5641 gattccgttt tgatcggtca ccgctgttaa tgacgttaat gtggcagagc cgctttttaa
     5701 ggcgaagtac acagtgagac cttcgatcag gttaccactg ccatcctcaa ccgttgtctt
     5761 taaggtactt aaatcagtgt tggtggcggc gatagtcgat ggatcagcga taaagctagc
     5821 aacatgggcg gtgcttgagt tgccagtgac tttcacggtt gtctgtgtct gtattgttac
     5881 gccgttatgg aacgatgcag tcaccgtata tgtaccgact ttattgctgg tcagaacgac
     5941 tggagcctga cctgaagagt tagttctcac ttttccgcca cttaacgtcg ccccttctgg
     6001 cgttacgcta aagttgatga cctgaccctc cactggagtg ccatttgcag aggttagcgt
     6061 tgccgtcaga gtagcacctg taggggcata gacaccgata agcggactgg acgccgtgag
     6121 tgtcagtttt tcatcaatag cctccagttg tttctcaagt gaggctccat tcgccaggga
     6181 tgctttcacc atatacgaac cgtttctttc gggcgtcatg gtgacctcgg ctacaccctg
     6241 cgtattagta gaaaccgtat tctggctaat gatcatttgc gaggatggct ctgcactgaa
     6301 tgtcacgggt tgatgcgcaa ccgggttgcc aaactggtcg ttaacgtgag ctttaactgc
     6361 tacgtcttgt gcgaccatta cctgaccttc aggtatctcc agactggtaa tcgtcgcaga
     6421 attaacatct gcactggcat tcagtaatcg cgagatgact tcagtaggtt tatctgccag
     6481 agaggctgaa actgttttca gtccgacctc ggtgcttgtc acaagaattt cagcgaaacc
     6541 ccgatcatca gtttcaacgc tggtgctgct tagcgtgacg gaggttccgc ggaaattaac
     6601 ttttatgcct tctaccgggt tgccgttagc gtcagtgacg cttgcagtca tggtcgcgcc
     6661 ctcagtcgtg ctgacgacga atgagtatac agaggttaag gaggctagtt ttgcggtacc
     6721 agcatcggca atcaacgtca cctgtttcgt atcactgaca ccataattgt tcacgctaac
     6781 tgtcacgggg taggtgccgg attttgtacc gctcagtgta acttcagcct tgccgttgat
     6841 gtcagtaatg gcggaaccgc cttgtccgag agtaaagctt gcgctcacat ctgccggtag
     6901 cgtgaatgtc accgcctcat tggctaacgg gttgccgttt ccatcagtca ctgttgcctg
     6961 cagcctggtc atcccgacgt tattagcgat aaaattgtcc tcggtaacgt taagattaac
     7021 ctgcgcagtg agcgtatccg caataaagtt caccaccaac tgctcactct taccgccagt
     7081 catcgatgct gtaacagtat gagcgcctgc ttttgtacct ttcagcgtga ctttcgcttt
     7141 gccttcagca tcagtaatcg ctttaccgcc atcgctcagc gtgaagttcg ccttcacatc
     7201 ttccggcaga gtaaatgtta cttcagtgtt ggctatcgca ttgccctctg tatcagcgac
     7261 tgttgctgtt agtgtcgtga gatcgttatt gtcggcaatc accggatcct tcgaggctgc
     7321 cagcgtgatt tcggcattcg cgacgttcgg cacataggtc actgtttgtt gcatcgaatc
     7381 gccattttcg agggttgccg tcacttgata aaccccggct tttgtagcgg taaagcttgt
     7441 gtaaaaattg ccgtcgtggt tggtagtata tatagcgtta ttactggagg tcacgccttc
     7501 acttggtgat actctgaggg ttacctcctg ctggggtaca ccgttgccgt aattatcctt
     7561 cacatcaata tacagattgg tagtgtcgcc tgcggagact gtatcaaaaa gtgcctgtag
     7621 caaggtgaga tgtgccgtag acgcatcagc gttgacatta attgatgtgc taagcgtgga
     7681 gctaccattt tccagactgg cctcaacggt atccggtctc gctcctgatt ctatcgagga
     7741 gcgggtattg gtataagtga cggtagcctt accctgttcg ttcgttacgg cttggccgcc
     7801 attcgtcatt tcggcagagt ttgttctgct ggtgaagttc acagtcacac ctttcaccgg
     7861 aaagccatta ttatcaacga ctgtggcggt gatgacgctg ccggagctgt tctgcggggt
     7921 accggcgatt atgctgtctg ggacaggcgt caactcgata atttttgccg ccgctgtgtc
     7981 gccaataaaa tgcacagttt tgttgtcgct ggcaccatta ttagccagtg atgcagtgac
     8041 cgtctgctca ccaaaggcaa cgcctgcgag agtggcctgc gcgatgccag actcattggt
     8101 attacttact cccggggtca gggtgagtcc tgaagaggct gagctgaatg ttaccggaag
     8161 attattcacc tcattgtcga actgatcttt aaccgttgca gttagcgttg cgttatcgac
     8221 gccattacct gtgatctcat cttttgacat ctgcaggaca acctgagccg aggttttgtc
     8281 cgccacaaac gttaccggct gcgaatcact ggtattgtta ttacccagcg ttgcggtaac
     8341 cgtatgcgta cccgcttttt tacctttcag cgtgacatgc gcttccccat tggcttgggt
     8401 gatggcaatg ccgttatttt cgagggtaaa gtttgccgca acgtcctgtg gcatggtgaa
     8461 ggtcaccgtt attcccgcca ccggatgatt cgacggatct ttcactgttg ctgtcagagt
     8521 cgtctcatcc acgccattcc caatgatttc cgctttcgat gtttgcagaa cgacaaccgc
     8581 tctgtcttta tcggccacaa acgtcattgg ctgtgtatcg ctgacattgc tgttagccag
     8641 acgagccgtg atcatatgcg tgcccgctaa cgtgccggtc agggaggcga ttgcaacccc
     8701 gttactatcc gtcatgcctt ttcctccgtt gctaattgag aactgacttg cgacgtcgtt
     8761 tggcacagag aaggtgattt ctttatctat tagtgggttg ccatttttat cctgcaaggt
     8821 tgcagtcata tgtagcggag ctgtgttggt gacggtgata tcacctgaag gcacactgag
     8881 ggtcagggct gcagtacttt gatcaccgat aaaaatcacc tgttgattag cctgagaacc
     8941 agagctcaca gaggccgtaa ccgtataatc accatttttc aaactggtca gcgtagctgt
     9001 ggcgatcccg tcgtggctat tcacttctgt ttggctcagt ttcgcctctg ctgaattaac
     9061 attgaaagtg accttgacgt cattgagcag gttgcctttt gcatcccgga ctgtcgcggt
     9121 cattgtggcg ctatcattgc cgtcagcgac cacttcattt ttcgacttct gcagctcaac
     9181 ctgcgcggta ctcgagtcgc cgacaaaact gacgattaac gtttgtttca cgccattttc
     9241 aagggtgact tcaaccgtgt tgtcttcctg cttactactt ttcagatcaa aagtcgccag
     9301 accattaaca tccgtttttg cggtgttttg attgttgaag gaagttgccg atccgcttaa
     9361 taccgcaaac gtgacggtat gatcattgat tgggttgctt ccttcatcag cgacattgac
     9421 cgagacggtg tttgctgcat tctcattggc gagcacacca ttattgctgg cagataatgt
     9481 cgcaattttt gctgactgcg ggttggcgtc gatgataaaa ccagcggtat gcaaatcttc
     9541 attccagttt tgcattaata gcttcgcagt aagcccactg cctttggtat aggcggtata
     9601 ggttgcctta tagacgccat ctgcggtttc tttccagtct gtagtgacac caggtttcac
     9661 gttgtcgatg ctgactgcgt tattcagttg ctgtttttgc tccttaacag gtttgtcatt
     9721 ttcatctctc agttctaccg tcacctcgat aggattgccg gagagataac ggtctttatc
     9781 aattttaatt gacgagtgag ttcgggatga cgaaatagaa atgatattca ccacggcggg
     9841 ggctttagcc gcatccacac cattcagctg tggcatcagc gtcagcgtgc cagacatcgc
     9901 tcctgtggtc aggatctggg tatagcttcc gtcaccatta tctttccatt cagaaagggt
     9961 gatgtcctga acaccttcgt gacgcgtcga gagcaccagc ccgacaacag gattacctgc
    10021 tgcatcatgc gcaataaaag tcagtgtggc ggttgaatgg gaatccgcgt tcaatgtttg
    10081 ggtacttaac gataccgagg aatctttctg gcttagcgta ggtgcctgaa cgaccaccat
    10141 gctctgttca cgattcgaca aattgccttt gacatcttcg gcggtgactt caatcggcca
    10201 ggtgttatcg gtttctggcg tactggtgaa ccggtaagcc ggcagggtaa ccagaatatc
    10261 tttacccgtt gtgaccactt tgccaccggc agcttccaga gcggtggctt cgacgttata
    10321 gcctttcagg gcatatttgg tttgtagcga cgaaaccaat gatttcactt ctcctgactt
    10381 ccctgtcacg gggtctgtca gggtcaggcg aaccagttct tttttgcggt attccagaac
    10441 gatgttgttg ttgcgatcca ccagatcata acggctgcct gcaaggctac gccgtgcagc
    10501 gacttcattc gggtcaagct gtttctgcat tgcgctgcca ggttgccagg taaaatcgac
    10561 ggcaaaacgg gtgtcatttt cgccctgttt accctggcgt tgctccgcgc tgaaggtcat
    10621 cagcgggaag ggggtatagt taagtccagc ggttatggca tgaggattac tttgccgatc
    10681 gtctttatcg aacagggcca cttcatcgcc ataatactgt tcatagacca gtttaccgcc
    10741 aaggtacggc caggcgggta gccagccttc tgcgcgtaca tcccagccat tggccgggcg
    10801 ggcttcataa tcgttgtcca gttcaggtgc gctgcgccag ttggtcagtc gcaaatagcc
    10861 gttactgctt aattttagat agtcgcgcca gtactccgcg ccaatgccgg cgcgggagtg
    10921 gtaacggcta agatcgtggt cgaaaaagaa gttgatgccc gacatccatg tgggagtgaa
    10981 atgacgccaa cctaagccgt tgttaatctg cgtacgctcg tcagtacgat ggagagtatg
    11041 ctgactgaaa aagagattat caggcgtttc ataccacgga tggagaaaat cgaactggga
    11101 gttcttcagg ctaaaatctt catccacgcc cagcgtgatt cttgcggtac cgaagcggct
    11161 taaccagtct gtcattgcgc ctgaagcctg agaagaggcc catccacgcg ccatatttgc
    11221 cgcttgctcg ctgttcatat cttcggcgag cagagacccg attggctgtg aagtactggc
    11281 tatctgttgc tcaaggttgc cactgctatt acccggtggc ggggttaaat tattttcact
    11341 aacttgtgcc gggacatcca gttcatcacc ctggcggaca ttatcaaaac ctcgagcaaa
    11401 cgtacgaaac tggttgagtt tgcgtaactc agccaccgaa ataccgaagc gttcggcaac
    11461 gctttgggcc gattccagcg ctccaagggt atagggtacc gtattggcat ttgcaatggc
    11521 gaattgtgca ggaactggtt gttgggttgc ggcgtttacc acaccttgtg ctgccgcagc
    11581 catagggaac gcaagttgag ttatcaggca gatacccgca gttaagcggc gtagtttaat
    11641 tcccatcccg cataatattt gtcggtcatt tatttcttct ccacttctct tcttcgtagc
    11701 catgcttacc ttccctgaac gagctaacat aaatgcgccc tttctagcac agcgaaaaaa
    11761 tgtaaatcct gatagcaata tgattgaaaa ttaataactt ttcgttaggc agttttgagt
    11821 gtgagttgca agaggggaga ctactgaata actcaagttt tataatcgag gggaaaatgg
    11881 tgatggcgtt catagcaaaa cgccctcaac cataaaggtc gagggcgctt aagatgttaa
    11941 aaacccgcta tccgttaaaa aacaatgttc aactaaggtc agtgacattg cgctaaaaaa
    12001 gcgaattttt agaatttggc tcctctgact ggactcgaac cagtgacata cggattaaca
    12061 gtccgccgtt ctaccgactg aactacagag gaatcgtgtg aacggggcgc atattactta
    12121 gcggtacctt gtctgtcaac actaaatttc ataagtcatt tcaattggtt aattaatctg
    12181 caaagttgtt aattaatgaa cattcgtcgc cgaaaacgag tctgtatcat tagtgcgatg
    12241 cagtctctgc aaaggatctt gttgataaaa ttggcagaaa cgttgccaca atgaagggaa
    12301 acgaggagca aaaagttctg gggcgctaaa gaaatattca gaaagtacgg caaaacattc
    12361 agcaggatca ctggcagcat aagcatcaat gctcgccgca ttctcgccaa ccaattcgat
    12421 ttcttcctga atgttgttca ttgcagcatg aagatcgtgt tcccagccag caacctcacg
    12481 caacggaata aagggaactc cgctggcgcg atcgccgtta cgggtgtcca gcttatgagc
    12541 gacttcatga ataatcaggt taaaaccaga agcatcaaaa gaatcttgta tatccaacca
    12601 gttcaaaacg ataggccctt gctgccagct ctgacctgac tgaacaatac gttggttatg
    12661 caccagaccg atatcgtctt cccattcatc atcgaccaca aatggcgcag gataaattaa
    12721 gacttcatga aaaccatcca gccattccag tcctaactcc agaacgggta ggcaaaatag
    12781 aagtgctatc cggcagcttc ttaatgaatc cagttcaaag ccctgtaaag gaacaagccg
    12841 cttttgctgt aaaaaacgtt cggcaagagt gactaatttg ctttgttcct gttccgtcag
    12901 acacgttaaa agggggatcg atagtgcttc ctgccaggga agggcagttt gatgtgctga
    12961 ttcttgtact ttccagggcc acttaatcat cgttttgctc gcaaactcgt cacttgaaca
    13021 aaattgcacg gacagggact gttaaaatgc caaatttcct ggcatcatgg caaccatctg
    13081 aacggagaga tgccggagcg gctgaacgga ccggtctcga aaaccggagt aggggcaact
    13141 ctaccggggg ttcaaatccc cctctctccg ccactttatc aatgacttat ctcccgactt
    13201 cccgccttgc ttttcctaaa cagaacaatc gtagaatatt cttgaagggt taggtcgtca
    13261 ctgttttctg ttcgatactg tgacattcag cacttgattc gctatggatc tgacaggaag
    13321 ctttcgagcg aaaatgtgca gttattcagt cgttttctta tcgctcacca ttattctttt
    13381 agacattcat cctatgaaac tgccgcaaag ttggtggtgg gaactgaagt tgcgtagaga
    13441 aggggtcaat aattgagtca aaaaaggaac gcggccgtca gattaaagcc gcgggacaaa
    13501 gtccataatt tccaagagtt gttgaatttc ccagcttttt tgaaagcctt tgtatcatac
    13561 taacaagtaa aaaaggttta aataccaaaa ttaacctgta tttttgataa gtcttaccat
    13621 tgtacgattt acacagtttc tgctcctcct tgttgatagt ggaaagggtg cattgtgcac
    13681 ttgcgtaaac atttatgacg cgttgatgtc tatggttcac atttccttgt caggggatcg
    13741 acttatttgc ggcgaggcat cattcgtaac agagtattat ctttctgaat gtagtgatga
    13801 aagagtgctg cgccagcgtg agctgcgatt aaaaagtaac ctatatttgc ccaggtttcg
    13861 tgaatatttt ttatcagtgc tttaatttcg ctgtttgggg taacaaaggg agacacattg
    13921 aaaccaagga aactccacga ttttccactg taagccatta aagcaatccc cagcagagga
    13981 agtgcaagga aggttatata aagcatgatg tgcattagcg aagccgcttt catttgccag
    14041 gcaggtggcg gtggtacaat agaaggatca ctataacggt gttttataat caggcgtgaa
    14101 aacattaaca cccaaacgaa tatacccgca ttgtaatgtg tttctcgcat cagcagataa
    14161 gtactactac ctttaggaaa ccagccacgg agttccatcg cggcataggt tattgcaacg
    14221 aaaagcagcg ttatccaatg taaataaatt tgagtttttg agaatcggtt catttttgta
    14281 gctcctgtaa tttttacagt caagctatcc cacgaacctt aaaaaatcct tatacagatg
    14341 tggttttaag aatttgttat ttaaaaaatt gatagtgcac attgtaacca gccaatataa
    14401 gcaatggcgt gagcacaggt atctttctct gttggccgta ttgtgtatgg aatccgttat
    14461 tggttaactc aatgcgggtt agcttattaa tatacagaca cgtcatagtg atgagcgtgt
    14521 ctgtagctag cttgcgttca gtggcgtttg tggatggacc agtggcagtt atgatgactt
    14581 actaaagcgg catcgaggcg ttatcatgag aatactcaat gagacatcat ttcctcgacc
    14641 acttcttcgc tactcaactg atatggataa tacgttggcc agttttccat ttcatttaat
    14701 aaagattgct gtgaatcatt acccataaaa atgtgaaaat gagatgattt tcgtggtgca
    14761 attatatgat cgctaaattg tatatattta ggggctttgc tttcaggatc tttacattcg
    14821 aataagtaac gaacgccttt cttgcctgat ttataggtga gtattttgta tccatcgtaa
    14881 tcatatttac aggatgttgt ttcattattt ctatggaatt caacaatgcc gtcctcaatg
    14941 ccaatcatct cgatatctgt tgcataacct ttgtgataat aatctttaat ttcagcaaat
    15001 gttttagttt tatctgcatc cgctttcttc tgaaagacgg ggtcaagttt gccactttgc
    15061 agtaaaggat aaacggattg ccagactcca tcccagtcac tgagcgttcg gttttgtaca
    15121 ttggcatcat caaaaacacc attagcagct ttttgttcga cctctgttaa gggtttgccg
    15181 tgtgagtgat gaccatgcga aaaggcagga gcgctaacaa taaagacacc taaagcaaca
    15241 gccagtttgt gaagacgaat cgccaaaaca gtttcctcca gagtcattgt atatgatgtg
    15301 taatgttata ttgtaacata tggcaaaatg caacaactct taggaaacga gagcatggta
    15361 ttctcaagtg cattcagcta tgttattgaa atagaataaa ttattctgga gatgagaggt
    15421 tgatgacaaa cgaaacataa cctgatgtga tctgcgcagg ccggacaggg cgctcgcgcc
    15481 acatccggca gggataacgc gtactttgtc ggcaatatga agttatacag ataatttgtt
    15541 gtgaacctgc ttgcgtaacc ggttaaaaag ggaacgcaac ttcttatacc gtaaggctaa
    15601 aagcagtaca gccagcccag cgtagatgag cggctgcggt gaaataatct tcactgacca
    15661 cagataatgt attggggcca ggatcgcgac aagatagacg aaattatgca actgttgcca
    15721 gtgcttgccg agttttcgct gcatcgcctg ggttgaagta aacgctaaag caagcaaaat
    15781 aacccagctg ataattccta acgttaaata aggccgggta attaactcct tgcctaataa
    15841 cgccagatta ttcacgccca gctccagcaa tgcgtaactg gttaaatgca gtgtcgccca
    15901 ggcaaagcac cataagccta acaggcggcg agtgcgtatc aataacggct gttttgcgta
    15961 gcgtgccaga ggtgtgatta acaaggtcgc cagcaaaaat ttcagtgcag tgcgaccggt
    16021 aaaatgctga atatctttca ccggatcggc acccagtcca ccgtgattga tcgcccagac
    16081 cagccagaga aacggcaaca atccggcaag atgcaggcta actttcagcc atgtcacctg
    16141 ttttgctgtc agacgcattc agaaattctc ccgcaaatcc aggccacgat acagcgatgc
    16201 cacctggtcg gcgtaaccat taaacagtag cgttggctgg cgctgaacat cgaggatgcc
    16261 gcctgaacca ataaatcgtt cggtagcctg tgaccagcgc gggtgatcaa catgcggatt
    16321 aacgttggcg taaaaaccgt attcgtcagg cgctgccaga ttccaggtgg ttggcggacg
    16381 ctcgcgggtc agcttaatac tgactatcga tttaatccct ttaaagccat atttccacgg
    16441 cacaatcagt cgtaccggcg cgccattttg tggcggtaac gccttgccat aaacacccac
    16501 ggtcatcagt gtgagcggat gcattgcttc gtcgagacgc aatccttcga cataaggata
    16561 tttcagcccg ccgccgataa agcggtcctg ctggccaggc atctgttccg gtgcataaat
    16621 tgtttcgaaa gcgacatact tcgcattgct ggtgggttcg gcaagcgcca gcaatttgtg
    16681 cagcggaaaa ccaatccacg gcaccaccat cgaccatgcc tccacgcagc gcatacgata
    16741 aatacgctct tccagcggga agcgacgggt taaatcatcg tgatcgaggg tcaatggttt
    16801 tgccacttcg ccgctgattt tcagtgtcca tggatcggtt ttcaggctac cagcattagc
    16861 ggcgggatcg gctttatcca gcccgaattc atagaagttg ttataaccgg agactttatc
    16921 tactggcgtc agtggcaggt tattttgcca ggcggcaggc ttgctgaact ccagcggttt
    16981 tccggcgggg gcgggcgggc gatcgttccc tttaaaccag ctaagcagat cggcatgcgc
    17041 agcgtgaggc aaagaaagtg cagctgcgct gatgcccagt gcttttaaca cctgtcgacg
    17101 cttcataaag aataccgact cggccgtaac atctgattct tttaaaaatt gattcttttt
    17161 cataagccat cacacttttt tgtcgttagc cagcaagagc cgtttactgt taatctgtca
    17221 gggagtgtcc tccctaacag tagcagagat ctgcttttac atatttatgc attaactgcc
    17281 acgataggtt gaatacccat attggctaag taataaaggc acatgataat gctcgttcac
    17341 tttattaata tgaaattcaa cggggatctc agggaagaaa ctttcaagat tttgtttctt
    17401 gaaatagtcc ccggttttaa atacgacacg gtaatcgccc gtagttgcag tttgctcggg
    17461 ccacagtgcc ttaattcgtc catccttatc tgttttggcg gtattaagtt gtaaccagcc
    17521 gttgtccgct ttcttttcaa gagtgactgt cacgtcggca gcgggttttc cggtttgctg
    17581 gttcaaaatg tgcacgctaa gaatgttttg ttgtgcggca taaaccaaag aaggtaatga
    17641 aaatgctgcc gttactacgg agagtactaa ataacgcttt aacatgttta tatccttgtc
    17701 atgtgaatga gttttgagga tagcgaaaaa gcggccaggg taaccttaca gcagcattac
    17761 aattttgtaa tgaaccaggc tgtttctata acatatgatt tatggcatat tattttcatg
    17821 aagattttac ttattgaaga taatcaaagg acccaggagt gggtaacgca ggggctttcc
    17881 gaagcgggtt atgtcattga tgccgtttct gatggcagag atgggcttta tcttgcgctg
    17941 aaggatgatt atgcattgat cattctggat attatgcttc cgggtatgga tggctggcag
    18001 atcttacaaa cgttaagaac agcaaagcaa acccctgtta tttgccttac tgcaagggat
    18061 tctgtcgatg acagagtcag agggctggac agtggagcaa atgattatct ggtaaaacct
    18121 ttttcatttt ctgagttgct ggcaagggtt cgggcacaat taaggcaaca tcacgctttg
    18181 aattcaacat tagaaatcag tggcttaaga atggactctg taagtcaaag tgtgagcagg
    18241 gacaatatca gtattacact gacgcgcaag gagtttcagt tactttggct actggcctcc
    18301 agagctggcg aaattatacc cagaacggtt attgcgagtg aaatttgggg aatcaacttt
    18361 gatagtgata ccaatacggt ggatgtcgcc attcgcaggc tccgcgcaaa agttgatgat
    18421 ccttttcctg aaaagctaat cgccacaatc cgggggatgg gctattcatt cgtagcggta
    18481 aaaaaataat gaaaagatta tctataaccg tccgtttaac cttgcttttt atattgctgc
    18541 tgtctgttgc tggcgctgga attgtctgga ctctctataa tggcctggca agtgagttga
    18601 aatggcgcga tgatacaaca ctcattaacc ggacagcaca gatcaagcag ttgttaattg
    18661 atggggtaaa tccagatacg ttacctgtat actttaaccg gatgatggat gttagtcagg
    18721 atatcttgat cattcatggt gatagcatca ataaaattgt taaccggaca aatgtcagtg
    18781 atgacatgtt aaataacata cctgctagtg agacaatcag cgcagctggc atttacagaa
    18841 gcattattaa tgatactgag attgatgctt tacgaattaa tattgatgaa gtttcgccat
    18901 cattaacggt tactgtggct aaattggctt cagccagaca taacatgctt gaacagtata
    18961 aaattaatag cattataatt tgcattgtcg ccattgtact ttgctcagta ttaagtccgc
    19021 tgttaatcag aacgggatta cgagagatca aaaagttgag tggtgtaacg gaagcgctga
    19081 attataacga tagccgggag cctgttgagg ttagcgcatt accgagagaa ctaaaacctc
    19141 ttgggcaggc gttgaataaa atgcatcaag ccttagtcaa agattttgaa cgcctaagtc
    19201 aatttgctga cgatctcgct catgaactta gaacgcccat taatgcatta ctgggtcaga
    19261 atcaggttac gctcagtcaa accagaagta tcgctgaata tcaaaaaaca attgccggta
    19321 acattgaaga gctggaaaat atttcgcggt taacagagaa catactgttt cttgcccggg
    19381 cagataaaaa caatgttttg gtgaaactgg actcgctttc tctcaataag gaagtcgaaa
    19441 atttgttgga ttatcttgaa tacctttcag acgagaaaga gatttgcttt aaggtcgagt
    19501 gcaatcagca aatctttgcg gataaaattt tactgcaacg aatgttatcg aatcttattg
    19561 ttaatgccat tagatattca ccagaaaaat cgcgtattca tataaccagt tttcttgata
    19621 ccaacggcta tcttaatatt gatgtcgcca gtcctggaac gaaaattcat gagcctgaaa
    19681 aactcttccg tagattttgg cggggagata attcgcgtca ttccgtaggt cagggacttg
    19741 gtctttcttt agtcaaagcg attgccgaat tacatggggg aagtgctacg tatcactatc
    19801 tcaataagca taatgtgttc cggattatgt taccgcaaag aaattaacat agggcttcag
    19861 tacgccataa atcaaccatg aaaaaacgca gccacgtcgt gagtattaac gtggctgcga
    19921 ttgattatgc gcttacattc atacgtaaca gggattaacc cgcataagct gccagcattt
    19981 cctgcgccgc cagtttaccc aacgcattcg ctgcgaaagg actgtcgccg gtgagaactt
    20041 tacggtcctt atgtactcgc ccggcgatgt cgtcattaat aatgttcatg cccattttct
    20101 tcagctcttc gccgaagtac caggtgagat gacccggcat atagcctatc tctggcgttt
    20161 gtttgtctgc ggcgtctggg aatgcgcaaa tggaataacc attcagtggg ttatcgccgt
    20221 ggcgaagcgc cagaaaagcc gccgggccgt ggcaaaggga gataacaaaa cggtcatttt
    20281 tgattgccca ctgtaaagca gcagccacgt cctggctttc tggtagacca ataagtgcgc
    20341 catgaccacc aggaacaaag attgctgcat attcgctatc agcgttgagg ctggcgacaa
    20401 catccgcgag tttcttcgga ttgcggaaca acgatttatg ctgctcaaag aatggcatta
    20461 ctttttcatc tttgtgcggc atagcccagt attcaaactt ggtcatcaga ccggaaatgg
    20521 tcgccacttc gaattcgaaa cctgcagcat ggagatgata caacggcagc aacgtttcaa
    20581 tcggatggtt accggtcgag aacagttttc cgttatcggt cggcaaataa cgttcgtccg
    20641 cggcgatcac cagaatttta tgtttaccgc gatacggttt tggatagtcc acgccatcaa
    20701 gatcagagac aggactggta tattggctaa gcgaatattc tgaagggaag aatgcattat
    20761 cttcagcaat atcgacctgc ggatttttac ttgtttgaac agtcatagtg tattccttat
    20821 tgttgcttag ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag
    20881 gctggaaaat tgagtcaata tatcgaaagc ggtagtcggc gggtgtaagt atgtaatgat
    20941 gatgctgcac tggtgttgaa tattattcac gccaaatttt ccttattgag caacgtcgtc
    21001 gcaagtggcc tcaatgattt tagaccatta tcagaggtac ggggcggcgg ggagagattt
    21061 agtgatgtgg aatattgtgg tgctgatgaa tatttatatc ccggattggt agagaaaaag
    21121 cttatttcat gcactatttt tgcaaatatt tctatatggg aggcggtttt gttgcagatt
    21181 aattcataaa gaaatgcatt tgggtgttgg ctggaagagt cgctggtaga tgttattgtg
    21241 ccagcagggg cagggtaagg tgctgtatcg atatgctttg gcaattgcgc gtgccgcaga
    21301 gggagataaa atgccaataa aaaagcctga gttcgatgag ggacgaactc aggctagtgt
    21361 taagttttac agtaacacgc cgcggcgcgt tggctgatta gaactggtag actaagccca
    21421 caccaacaac gtcatctgtt gcgatgccat tgttgtcata gaaatcgtca tctttatcca
    21481 acaagtttat tttatagtca acatatgttg agaagttctt attgaaatag taggtcgctc
    21541 caatttcaac atagttgacc agatcctgat caacatactc ttgatgacca ttgttgttat
    21601 agtcgttacc gccgaggtct ttgccttttg aataaacata tgcaagagat gggcgcagac
    21661 caaaatcgaa ctgatactgt gctacagctt caaagttctg ggttttattt gcgataccca
    21721 cattaccata tggtgtcata ttgcgggttt cagcatacat cattgcaaga taaacattgt
    21781 tggcgtcgta ttttgcacca aatgtccatg cttcagctgt atcaccacca gcaaatttgc
    21841 tgtattcatt gtagtgatac ccgccaagag ctacttgaga attggtacgg tcagagtttg
    21901 agtaagcggc acctaagctc aaaccgaagt caaagtcata tgaagtggaa agaccaaaac
    21961 catcaccgtt ctggaagcga acgtcttcgt gaccattttt agtaccttct tgatcttctc
    22021 ctgcattgct gccttcgttg ttaccttgat actgcaatgc aaagttcaga ccttccacca
    22081 gaccgaagaa atcactattg cggtaagtag ctaccccatt tgcacggcct gccatgaagt
    22141 tatcagtcca ggtataggag tcgccgccga atacaggaag catatctgtc caggcttcaa
    22201 cgtcgtaaat cacaccatag ttacgaccat agtcaaagga gcccatctcg ccgaacttca
    22261 gacctgcaaa agcaagtttg acataagtat tatcagaacc ttcagtgtcg ctagcctttg
    22321 ttttggtcag tgcttgacca taaccagtta actgatctgt aatttgggtc tcacctttga
    22381 agcctaactc gatgatagtt tcatcgccgt catcgccagg gttatcggaa aaagtatgac
    22441 gagcatcaac ttttccgtac agatccagtt tattgccatt tttattataa atttcagccg
    22501 catttgctgc gccagcaact aataacgccg ggaccagcat tgccagaact tttcttttca
    22561 ttatgtattc ccttgtgatt ataatcttca tgaatatatc aataagtgcc gttatccaaa
    22621 a
//
LOCUS       DAZGSP010000081        22535 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZGSP010000081 DAZGSP010000000
VERSION     DAZGSP010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.081"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(89..1339)
                     /locus_tag="ACHK74_003488"
     CDS             complement(89..1339)
                     /locus_tag="ACHK74_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP0222240.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(1433..2371)
                     /gene="psuG"
                     /locus_tag="ACHK74_003489"
     CDS             complement(1433..2371)
                     /gene="psuG"
                     /locus_tag="ACHK74_003489"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFP0222241.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELENTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(2359..3300)
                     /locus_tag="ACHK74_003490"
     CDS             complement(2359..3300)
                     /locus_tag="ACHK74_003490"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFP0222242.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVLGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVIGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(3724..5415)
                     /gene="fruA"
                     /locus_tag="ACHK74_003491"
     CDS             complement(3724..5415)
                     /gene="fruA"
                     /locus_tag="ACHK74_003491"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFP0222243.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(5432..6370)
                     /gene="fruK"
                     /locus_tag="ACHK74_003492"
     CDS             complement(5432..6370)
                     /gene="fruK"
                     /locus_tag="ACHK74_003492"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP0222244.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(6370..7500)
                     /gene="fruB"
                     /locus_tag="ACHK74_003493"
     CDS             complement(6370..7500)
                     /gene="fruB"
                     /locus_tag="ACHK74_003493"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFP0222245.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            7868..9049
                     /gene="setB"
                     /locus_tag="ACHK74_003494"
     CDS             7868..9049
                     /gene="setB"
                     /locus_tag="ACHK74_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFP0222246.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(9046..9300)
                     /locus_tag="ACHK74_003495"
     CDS             complement(9046..9300)
                     /locus_tag="ACHK74_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP0222247.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            9455..10027
                     /gene="yeiP"
                     /locus_tag="ACHK74_003496"
     CDS             9455..10027
                     /gene="yeiP"
                     /locus_tag="ACHK74_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFP0222248.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            10250..11716
                     /locus_tag="ACHK74_003497"
     CDS             10250..11716
                     /locus_tag="ACHK74_003497"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP0222249.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            11834..12820
                     /gene="yeiR"
                     /locus_tag="ACHK74_003498"
     CDS             11834..12820
                     /gene="yeiR"
                     /locus_tag="ACHK74_003498"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFP0222250.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            12859..13572
                     /gene="lpxT"
                     /locus_tag="ACHK74_003499"
     CDS             12859..13572
                     /gene="lpxT"
                     /locus_tag="ACHK74_003499"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFP0222251.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            13984..14550
                     /gene="mepS"
                     /locus_tag="ACHK74_003500"
     CDS             13984..14550
                     /gene="mepS"
                     /locus_tag="ACHK74_003500"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFP0222252.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            14731..16287
                     /locus_tag="ACHK74_003501"
     CDS             14731..16287
                     /locus_tag="ACHK74_003501"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP0222253.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            16369..18183
                     /locus_tag="ACHK74_003502"
     CDS             16369..18183
                     /locus_tag="ACHK74_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP0222254.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPSEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            18184..19278
                     /locus_tag="ACHK74_003503"
     CDS             18184..19278
                     /locus_tag="ACHK74_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFP0222255.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGIRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            19278..20367
                     /locus_tag="ACHK74_003504"
                     /pseudo
     CDS             19278..20367
                     /locus_tag="ACHK74_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            20369..21958
                     /gene="yejF"
                     /locus_tag="ACHK74_003505"
     CDS             20369..21958
                     /gene="yejF"
                     /locus_tag="ACHK74_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFP0222256.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(21962..22306)
                     /locus_tag="ACHK74_003506"
     CDS             complement(21962..22306)
                     /locus_tag="ACHK74_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFP0222257.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
BASE COUNT         5433 a         5860 c         5519 g         5723 t
ORIGIN      
        1 cgcagcacat gcaacttgaa gtatgacgag tatagtacgc gattgccgga tgcaacgctt
       61 aataagcgag tacatccggc aatgaaactc aagctaaacc aataaagaac ccggcaatgg
      121 tcgcgctcat caggttggaa agcgtcgccg ccgccagcgc ccgtaaacca agctgggcga
      181 tttccggcgc gcgttttggc gaaatagccg aaaacgctcc aacgacaaca ccgatagaac
      241 caaagttagc aaaaccacaa agcgcaaagg agataatcgc aatggttttc acttccagcg
      301 tgccgcccgt ttgcaggtat ggggagaaac tcaggtaagc gacgaattcg ttaatcgcca
      361 gtttctgccc aatcaggcta cccgcaaggt tggcatcact ccagtccacg cccataatcc
      421 acgccagcgg tgccagtaca tagccaaaaa tactttccag agaggcattg gcgaaaccaa
      481 accagccgcc aattccgccg ataatgccgt tgatcagcgc aataattgcg acaaacgcca
      541 ttaccaccgt cgccacacca gcggcgattt ttagcccggt catcgcaccg ctcgccgccg
      601 cttcgataaa gctttttggc ggcgtttcgc tgaacgacag attttcaaat gtgacctgcg
      661 aaggctcggt ggccgggcta agaatacgtg caaacaaaat accacctggg atcgccatca
      721 gcgatgccgc taacaggtag tcaattggta cgcccattcc ggcataacca atcatcatcg
      781 aaccagcaat ggacgccatc ccgctacaaa ttgcggtaaa caactcgttg cgattcatgc
      841 gatcgataaa cggtttaacg atcgccggga tctcattttg cccgaggaaa atagttgtga
      901 ctgcgacaaa agattcgatt ttgctgatgt taagagcttt ctggaaaatg ctgccaagga
      961 tgcgaatcag cagccccatc acgccaatgt agtacagcag actgatgagc gcagtaacga
     1021 aaataatcgc cggaagtacg cgaaaggcga agataaaacc cgcaccgtca aacaggacat
     1081 ccattttcgg cccaaccagc gaaccaaaaa tgaaggcgct accggcatca ctgtaagaca
     1141 tcactttatg aacgcctaat gccgcctgtt ctactgccca ctttcccggt gggaagtaga
     1201 gcatgatgcc accaatagcg atttgcagca gcagtgcggc tccaaccgtg cgcaaactga
     1261 tgctcttttt attcactgac aacagaaatg ctattgccag taacaccacc atccccacaa
     1321 cacttctcat tatatccata atgattttcc cttcatgcca gtaaacccgg cgtcagcgcc
     1381 aggttttggt atgcttgatg agtacgggcg acggctttct gcccgtcaga aattaacccg
     1441 cgagacgctg gtattctttg gcaatttcac tcgccagaat ggcgttgttg aacaccagct
     1501 ggatattgga tttcaggctg tcaccgccgg tcagctcagc aacgcgcgcc agcaggaacg
     1561 gtgtactttc tttaccaata acgccctgct cttcggcttc ggcgacggct tgatctatgg
     1621 ctgcattgat agattcctcc ggcatcgcaa actgttccgg gatcgggttc gctaccacga
     1681 ggccaccgtt cagcccgctt tgccatttca ccgccattgc acgggcaatt tcactggcgc
     1741 tgtcgagacg aatgctgacg tcaaacgagc tggtacggca gaaaaacgca ggcagcgctt
     1801 tagtctgata gccaattaac ggcacaccga aggtttctaa atactcagtg gttaatccta
     1861 aatcgagaat agatttcgcc ccggcacaaa caacggtgac attagtattt tccagttctt
     1921 gcaaatcggc agaaatatcg aaggtatgtt ccgccccgcg atgcacacca ccaattcccc
     1981 cggtggcaaa tactttaatt ccggcaagcg ccgcaataat catcgttgaa gccacagtgg
     2041 ttgcgccatt ttttccggcg gcaacaacaa aaggtaaatc gcgacgacta actttggtca
     2101 cgttatgccc ttcacgaccc agtaattcaa tttcttcttt gcttaaaccc actttcatca
     2161 cgccgccaat aatggcgata gtggcaggta ctgcgccctg tttacgaata gtttcttcta
     2221 cttcaattgc ggtctgggca ttttgtggga acggcatccc gtgagaaata atggtcgatt
     2281 ccagcgccac aaccggtttt ttgtttttta aagcgtcctg cacttccggg gaaatttgta
     2341 ataattcagg ggaaattttt aattcagaca ttctgcgttc tccactaacg atataacgtt
     2401 ggcaatcgat aaatcggggt tattggtgta ttcacaggag agcgccattg acgaacatcc
     2461 ctgtgcgaaa cgaacagatt cggcaaacgg cattccgtct acccaacacg aagcaagtcc
     2521 cgccatcatg gcatcgcccg ctccgataac attaataaca ttggttttga tcggcgcaga
     2581 ccagccattt tcaccgctga tatcgctgta ataaacgccg tcgccgccca tgctcaatac
     2641 cagtcggttc aggccatgtt gatggaacca ggcagcaact tttgccacat cgtcacgccc
     2701 tgacagcgca atcccactca gggtttccgc ttcaaggcgg tttggcttga gagtgtggat
     2761 ctgatttaga cggtcgcgca ctttgacaca tttccacgct gaaaccggat cgacaaatac
     2821 gggtacgttg gcggcattat ccagaatcca tgccagtgcc tcttcactga tattacagtc
     2881 tgcgacaatg acctttgccc tctgaataaa ttcacggtgc tgtgcgagat attcagctgt
     2941 aatagcgttg ctaatattca tgtcatttat agcaaccagc atttcaccgg tattatcgag
     3001 taatgataaa tagcttgacg tattttctcc tagcacaatc aggcatttat cgacataaac
     3061 gccagattga ttggtttgcg ttagcagcga ttgaccataa aagtcactgc ctacggcgct
     3121 cagtagccag gctttgttcc ccagcaacgc caggttttgt gcaatattgc gcccgactcc
     3181 accaggcgta aattttattt tacctggatt tgaatccgca taatttaatg attcatgtga
     3241 atatccggcg acatcaatat tcgccgagcc tataattacg acataatcct tttcgcgcat
     3301 agcacgtcct ctggtaaaaa attaccaacc ctgtcaatcc acaagaatat agatactgca
     3361 aagacggttt aaacatgtgt tcatatttaa acatgtgctc atagtatgca tgttttacgt
     3421 aaagtaaatt accgccagag aggcaaaatg ggcagatttg agcaggctca caaaatgcga
     3481 ttaaatgcct gaattacaaa agcgaggaat ttgtgacggg atgcacagaa caggaacaat
     3541 aaaaaagggc agaaaataat ctgcccttga ggaaataacc ttagtgtttg atgaacgttt
     3601 caagtttcta ctgaaagata aaaccattta aattcataaa attacattca caagctggtc
     3661 attccctttg cgctcatcca atgatacagg gcaggaaatt tctgccctgt aacacacctt
     3721 ttattacgct gctttcgcta ctgcgtccac ttccggacgt ttcaggaagg cataggccaa
     3781 acccgccacc agcgtaccgg caataattgc taccaggtaa cccagtaccg gcgtaatagc
     3841 gccagggatc agcagaacaa acagaccacc gtgtggtgcc atcagtttcg caccaatcgc
     3901 cattgagatt gcgccagtca gcgccccacc aacgatacag cacggcagca cacgcatcgg
     3961 atcacgagca gcaaacggaa ttgcaccttc cgaaatgaag cacagtccca ataccagagc
     4021 ggctttgcca ccttcctgct gcgctttgtc gaatttgcga cgcgccacca ttgttgccag
     4081 acccattgcc agcggtggca ccatacctgc cgccataatc gccgccatcg ggccataggt
     4141 ttgagtactc agcagaccca caccgaatgc gtacgctgct ttgtttaccg gaccgcccat
     4201 gtcagtacac atcatgccac cgaggatcgc ccccagcaga accgcattcg cagtccccat
     4261 ggtctgcagc cagtgagtca acccttcgag aatgccagca actggtttac cgatcaggta
     4321 gatcatcgcc agaccgacca ccagactgga aattagcgga atgatcagga tcggtttcag
     4381 cgcctccata ctctgtggca gtttcagttg cgtactgatt aacttcgcaa tgtaaccagc
     4441 caggaagccc gcaataatac cgccaatgaa gccagaaccg gtgctgacag ccagcatacc
     4501 gccaatcaga cccggtgtga ggcccggacg atcggcaatg gagaaggcaa tataacctgc
     4561 cagtaccggc accatcagcg caaaggctga accaccgcca atctgcatca gcgccgctgc
     4621 caacgtaccc ggctctttaa acgcttcgat accaaaagca aaagaaagcg cgatacacag
     4681 accaccagca acgaccatcg gcagcatgta agaaacgccc gtcagcaagt gacggtaagc
     4741 gcccgcactc tctttcttac cttcagtggt cgccgtttga gctttgcccg ccggttcata
     4801 cggcgttgct tcagcaaccg ctttatccag ttcctgcgcg gttttcttca gcgccagacc
     4861 ggtagaggta cgatacatcg gtttaccagc aaatttcgcc agatccactt cgatatctgc
     4921 cgccacaatc accagatccg ctgcggctac ttcctcggga gtgattgcat tacccgcgcc
     4981 aacagaacca cgggtttcaa ctttcaccca ccagccacgt tttttcgctt cggtttcaat
     5041 ggcttcagcc gccataaagg tgtgtgctac gccagtcggg caagcagtca ccgcaactac
     5101 gcgtttcgga ccgctggcgg caaccggtgc tgtcgcagtg accggcgcag tgtaaggttt
     5161 cgcatggcct ttggcttcac tcaggaacag ctcagggtgc gcaactgccc gggaaatatc
     5221 acccagccag acatttttac cgttcagcgc gctgtcattc gggatggaat caccgagaac
     5281 aatcgccatt tctgcgtcgt tcggattgtc gatgatttcc agttttgctt ttcgcgccgc
     5341 cgcgcccagc agggtcttcg ccatataggc gcgtgcctga ccgagattag cgtcaataat
     5401 cagcagcgtt ttcattatgc ctctcctgct gtcagttaaa aggttgtaag tcgacgcgcg
     5461 ccatcattgc ggccaactgc ggacgatcgg taatacccac attgctttga cttaccgcca
     5521 gggcggcaac agctgtcgcc agacgcagtg tgtgttcact ggattcacgc atcagcaagc
     5581 cataaatcag gccaccaacc atagaatccc ctgcgccaac ggtgcttacg acatcgactg
     5641 acggtggttt ggcgatccat tcgccggagg cattaaccca aagcgcgcct tcggcaccca
     5701 gtgaaataac aacatgcgca ataccttgtt cacgtagcgc atgtgcagct tcaatcacat
     5761 ctttcatttc aggcagttta cggcctgccc agatttccaa ctcgcggcgg ttaggtttca
     5821 ccagccacgg tgccgctttc aaacctgcta ctaacgcttc acggctacta tcaaagataa
     5881 tgcaaggaca ctgactacgc aggcgagtca tccagtcggt gaacgcttcc gggctgacgc
     5941 ctgacggtaa gcttccgctg acacagacca tatcgaactg accgagccag ctcagagaat
     6001 cagtcacaaa gcgttcccag tcggcggggg tgacttcaaa acccgagaag ttgaagtcgg
     6061 tcacttcgcc gtctttttcc gtcagcttaa cgttaattcg ggtgcgcccc tgtacaacct
     6121 ggaaacggtt ggcaatgccc agctcgctga acagttgctg aaaaccatcc tgattgtctt
     6181 tacccaggaa gccgccaacg gtgacatcaa ttcccaggtc ttttaatact ttggccacgt
     6241 tgatgccttt acccgccgca tgcagaccgg tggttttcac caggttcact tcgccgcgtt
     6301 caatttccgg gcagaaacca acaaggtcat aagccggatt aagggtgata gtagcaacac
     6361 gtctgctcat tatgcgccct ccccaagacc agcagcgata gcgtcgccaa tggctttcag
     6421 cgcctgttca gcatctgcac cctgggcggt aaagcgtagg cgatgacctt tcttaacgcc
     6481 aagtgccaca actttcatca gactacgtcc gtttgccggt ttgccggtac catcaaggtt
     6541 tgtcacggta atatcactgt taaattgttt aatggtattg accagcatgg tacctggacg
     6601 agcatgcagg ccgtgttcat tgcgcaccac aaactccgcg cttaacacgt cgtcggtcgg
     6661 cgcatcatcg ctggtcagca gcgccagcaa cgttgccgca tctgctttca gcaagcggtc
     6721 agctttattg tcgagcaata aatcagcgag acgcttaaga accgcgatgg gctgatcgtc
     6781 attcatcgcc acactcacca gcatggctgc cgtttcgccg tccacatcaa aagcatttgc
     6841 cgcacggctt accgcaatcg cgctacgcag attgccttcg gcgctatcgc tcagccagat
     6901 accctgtccg agattcagcg gttgttcatt gatggctttg gtgacgaaag tggcgtcaac
     6961 tgcccccgcc tctttcagac gcgcagcgtt cagcgcctga agagtcagca gatcgctggc
     7021 gacgatatcc agcgtcagca tttcgttgtc gagcttcagc tgctcactct gcttttcgcc
     7081 catcagtaat gcgcgaagtt cttctgctgt tgttgctgac ttcagttgtt cagcaacgga
     7141 atcatcgctc agtacgtggg tcagctggcg tagcaggccc agatgttcat ccgagctggc
     7201 agcaataccg attgccacgt acgctacctg accgtcaccc caggtgacgc cttccgggaa
     7261 ctgaaatacc tgaacgccgg ttttcagcac ctgatcgcgg gtgtcggtag tgccgtgtgg
     7321 aatagcaata ccattgccga ggaacgttga ggtttgctgt tcgcgcgcca gcatgccatt
     7381 gacgtagcct tctgctacat taccggcctg caccagcgcc gcagcgacct ggcgaatcgc
     7441 ctcttctttg tctccggcct tttcgcccgg atggatgtcc tgtacggata actggaacat
     7501 agttctcctc tcttgctgaa ttgaaacgat tcagcctcta tgagaaaaaa agcgccaacc
     7561 tggcttaggg ttaaagacaa gatcgcgctg aaacgtttca agaaagcata atacttctgt
     7621 ttcagcacgc aaggaaagcc gtaaagttat gagcaaaagt ttgatgtgct gcacaatttt
     7681 ttgccatttt tcctaattgc tgatgggaaa atccgttatc agcaatttca tttcagcagc
     7741 gtcagcagcc aatcagcaac agtgaaatgg cattttgatt attcgtggcg cttttgattt
     7801 cgctgtctgt ttattttctg acaagcagcg taaactccgc gtcttcctct tccagtgatc
     7861 gaccagcatg cataactccc ccgcagtctc cagcgcgaaa tcgtttgacc tgacctcgac
     7921 ggcgttttta atcgttgcct ttctcaccgg tattgcgggc gctctgcaaa ccccgacact
     7981 cagtattttt cttaccgatg aagtacatgc ccgtccggcg atggtgggat tcttctttac
     8041 cggcagcgct gtcattggga ttctggtaag tcagtttctc gccgggcgct ctgataagcg
     8101 cggcgatcgc aaatcgctga ttgtcttttg ctgcctgtta ggcgtgctgg cctgcaccct
     8161 ttttgcctgg aatcgcaact actttgtttt gctattcgtt ggcgtctttc ttagcagctt
     8221 tggctcgacc gctaacccgc aaatgtttgc ccttgcccgt gaacatgccg acaaaaccgg
     8281 acgtgaggcg gtgatgttca gctctttttt acgcgctcag gtttcactgg catgggtcat
     8341 tggcccaccg ctggcttatg cgttagcgat gggtttcagc tttacggtaa tgtatctgag
     8401 cgcagcggta gcgtttattg tttgcggcgt gatggtgtgg ctgtttttac cgtcgatgca
     8461 aaaagagctt ccgctggcga ccggcacggt tgaagcgccg cgccgtaacc gtcgcgatac
     8521 gctactgctg tttgtcattt gtacattgat gtggggctcg aacagcctgt acatcatcaa
     8581 catgccgcta tttattatca acgaactgca tcttcccgag aaactggccg gtgtgatgat
     8641 ggggaccgcc gccgggctgg aaatcccgac gatgttgatt gccggatatt tcgccaaacg
     8701 tctgggtaag cgtttcttaa tgcgcgttgc tgccgtgggt ggcgtctgtt tttacgcagg
     8761 aatgctgatg gcgcattcac ctgtcattct gttgggcttg cagctgctaa atgctatttt
     8821 tattggcatt ctgggcggca tcgggatgct ctattttcag gatctgatgc ccggtcaggc
     8881 gggttcagcc accacgctct ataccaacac ttcgcgcgtg ggctggatca tcgcaggatc
     8941 agtggcgggc atcgtcgccg agatctggaa ttatcacgct gtgttctggt ttgcgatggt
     9001 gatgattatc gccactctgt tttgcttact gcggattaaa gatgtttaag gcgcggtcag
     9061 catttccaga tcgataagcc aggtcatggc ctgctgacgc gagttaccgc acatttcagc
     9121 gctggcttgt aagcctgcac agactttcgg tcgcagtggc gaggtgaaaa ttttacagcg
     9181 ttgctgttca tcaagctgaa tgcagggcgt attggcgggc ttgccatctg gcataccggg
     9241 aatagggctg gaaattgaag gggcagtgca acacgccccg caacccggac ggcattccat
     9301 acgttgttct cttaaggtta accaccggat tgatgagcgc gcacagtacc accttttttg
     9361 caccagcaaa agtgcgaata ccacttgccc gaaaggcccg tcgcgagtac tttgtcgcga
     9421 tatttttgac attttcgact acaggaattt ttcgatgcca agagcgaacg aaattaaaaa
     9481 aggtatggta ctgaattaca acggcaaact gctgctggtt aaggatattg atattcagtc
     9541 gcccactgcc cgcggtgccg ctacgctgta caaaatgcgt ttttctgatg tccgtaccgg
     9601 gctgaaagta gaagagcgtt tcaaaggtga tgatatcgtt gacaccgtga cgctgacccg
     9661 ccgttacgtt gacttctcct atgtcgatgg caacgaatat gtctttatgg ataaagaaga
     9721 ctataccccg tataccttca ccaaagatca gattgaagaa gagttgctgt ttatgccaga
     9781 gggcggcatg ccggacatgc aggtgctgac ctgggacggt caactgctgg cgcttgagct
     9841 gccgcagacc gttgatctgg aaatcgttga aaccgcacca ggcatcaaag gggcatctgc
     9901 cagcgcccgt aacaaaccgg cgacattgag cactggtctg gtgattcagg taccagaata
     9961 cttaagcccg ggcgaaaaaa ttcgtatcca tatcgaagaa cgccgttata tggggcgtgc
    10021 tgactaactt cagccgcatg cagaaaaggg atagctcagg ctgtcccttt tttaatttat
    10081 tataccaatc ttctattttg cgctttctga acaccttacg ccctcaattt tcactcgttg
    10141 attgatcgcc ctcacacttc atcgcattaa caatccagac caatttcaat tgctgtcata
    10201 taactttaca ctgtcattgt taatttatcg ttactaagac gtgactccta tgaagacaat
    10261 tgcctccgtt acgctcccgc atcatgtaca cgctccacgc tacgatcgcc agcagttgca
    10321 atcacgtatc gttcattttg gctttggcgc ctttcaccgc gcacatcagg cattactgac
    10381 cgatcgtgtg ctgaatgccc agggcggcga ctgggggatc tgtgaaatca gcttgttcag
    10441 cggtgatcaa ctgatgagcc agctccgcgc acagaaccat ttatataccg tgctggagaa
    10501 aggtgcggac ggcaatcagg tgataattgt cggtgccgtt cacgaatgcc ttaatgcaaa
    10561 actggattcc ttagcggcaa ttattgagaa attttgcgag ccacaggtgg caattgtttc
    10621 cctgacgatt accgaaaaag gctattgtat tgacccggcc accggtgcac tcgacaccag
    10681 taatccgcgg attattcacg atctacaaac ccctgaagaa cctcactccg caccgggtat
    10741 tctcgtcgaa gcactgaaac gccgccgtga gcgcggcctt acaccgttta ccgtgctctc
    10801 ctgcgacaat attcccgaca atggccatgt ggtgaaaaac gcggtgctgg gaatggcaga
    10861 aaaacgttcg ccagaactcg ccgggtggat aaaagaacac gtcagttttc cgggaaccat
    10921 ggtcgaccgc attgttccgg ctgcaaccga cgaatcactg gcagaaatca gccagcatct
    10981 gggggtaaat gatccctgcg cgattagctg cgaaccgttt atccagtggg tggtggaaga
    11041 taacttcgtc gctgggcgtc ctgcctggga agtcgcaggt gtacaaatgg tgaatgatgt
    11101 cctgccatgg gaagagatga aactgcgtat gcttaatggc agccactctt ttctcgctta
    11161 tctgggttac ctttcaggat tcgcccatat cagtgattgc atgcaggatc gcgcatttcg
    11221 ccatgccgcc agaacattaa tgctgaatga gcaagcgccg acactacaaa ttaaagatgt
    11281 cgatttaaca caatatgcgg acaagttaat tgcacgtttt gctaatccgg cgctgaaaca
    11341 taagacctgg caaatcgcaa tggatggtag ccagaaatta ccgcaacgta tgctggcagg
    11401 tattcgcata catctggggc gcgaaacgga ctggtcgttg ctggcattag gcgttgctgg
    11461 ctggatgcgt tacgtcagcg gcgttgatga tgccggaaat gccattgatg ttcgcgatcc
    11521 gcttagcgat aaaattcgcg aacttgttgc gggcagcagc agtgaacaac gcgtaaccgc
    11581 cctgctttcc ctgcgtgaag ttttcggtga tgatctgcca gataacccgc attttgtgca
    11641 ggccatcgaa caagcctggc aacaaatcgt acaattcggc gcacatcagg cgctattaaa
    11701 caccctcaaa atttaacgat ttctgcggtt aaagcggatg aagctcacct tcgtccgctc
    11761 tccccttctc ttttctgcct tttttagcca ggattaacgc tcagttaact taccagagta
    11821 ttgcggagcc gttgtgacca ggaccaacct catcaccggt tttctcggca gcgggaaaac
    11881 cacgtcgatt cttcatctgt tagcccataa agatcccaac gaaaaatggg cggtactggt
    11941 taatgaattt ggggaagtcg gaattgatgg tgctttgctc gccgatagcg gcgcattgct
    12001 gaaagagatc cccggcggct gcatgtgctg cgttaatggt ttacccatgc aggtagggtt
    12061 gaatacctta ctgcgtcagg gaaaaccaga ccgcttgttg atagagccga ccgggctggg
    12121 ccatccgaaa cagatcctcg atctgttaac cgcaccagtc tatgaaccgt ggatagatct
    12181 gcgcgccacc ttgtgcattc tcgatccacg cctgctgctg gacgaaaaaa gcgccagcaa
    12241 tgaaaacttc cgtgaccagc tggctgccgc agacatcatt gtcgccaata aatccgaccg
    12301 tgcgacgccc gaaagtgagc aagcgctaca gcgttggtgg cagcaaaatg gtggcgatcg
    12361 acaattaatt cacagtgagc atgggaaagt tgacggtcat cttctggatt tgccgcgtcg
    12421 caatttagcc gagttgcccg ccagcgccgc gcattctcat cagcatgtcg tgaaaaaagg
    12481 gttagcagcg ttaagcctgc cagagcatca acgctggcgt cgcagtctga acagcgggca
    12541 aggatatcag gcctgcggct ggatattcga cgctgatacg gtattcgaca ccattggcat
    12601 tctggaatgg gcgcgacttg caccggtgga acgcgtcaaa ggcgtgctgc gtattcccga
    12661 agggctggta cgaatcaacc gtcagggcga tgacctgcac attgaaacgc aaaacgttgc
    12721 gccaccggac agccgtattg agctgatttc cagcagcgaa gctgactgga atgctttgca
    12781 gagcgcgctg ttgaagcttc gtttagcgac taccgcgtaa ggttgcctgc gtttttcagt
    12841 aagataatta gagaaaatat gattaaaaat ttgccgcaaa tagtgttgtt gaatattgtc
    12901 ggcctcgcgc tgtttctttc ctggtatatt cccgttaatc atggattctg gttgccaatt
    12961 gatgcggata ttttttattt ctttaatcag aaactggtcg aaagtaaggc ctttttgtgg
    13021 ctggttgcat tgaccaacaa tcgcgctttc gacggttgtt ctctgctggc gatgggaatg
    13081 ttgatgctga gtttctggct gaaagaaaac gcccctggca gacgacgtat cgtgattatg
    13141 ggtctggtca tgctattaac tgcagtggta ttaaaccagc tgggtcaggc attaattcct
    13201 gtaaaacggg ccagcccaac attgactttt accgatatta accgcgtcag cgaactgctc
    13261 tctgttccca cgaaagatgc ctcacgagat agctttcccg gcgatcacgg catgatgctg
    13321 cttatttttt cggcattcat gtggcgttat ttcggcaaag ttgcaggcct tatcgccctt
    13381 attatttttg tggtttttgc atttcccaga gtaatgattg gcgcacactg gtttactgac
    13441 atcattgtcg gttcgatgac cgtgatattg atcggtttgc cctgggtgtt gctgacgcca
    13501 ttaagtgatc gattaatcac cttttttgac aaatcactac cgggaaaaaa caaacatttc
    13561 caaaacaaat aactcacagt aattaacatc atcaggctta tttttatagt gaggataatc
    13621 ctgatgatgc gcaccgtgct ttcatctatc gaacgcaaaa atcattctct aagtaaatga
    13681 atggattgca tgcgtttcac tcaatcgtac tttaattgac caatcccgct tattaacttt
    13741 ctgtatcact ttttcttata aaaaatcatg taaaaccgct cgccaagacc gcaccaatcg
    13801 ggtaatctcg aactcgtttt gcctcggcgg tagattatcc tcacagcata taattttgtg
    13861 cgttagtcca cagatttggc cttaaggaat tgtttcaaca tgcccaggta attagtctcg
    13921 tgtcgcttgg cattttttta taacgatatt tgtcgttaag gacttcaagg gaaaacaaac
    13981 aacatggtca aatctcaacc gattttgaga tatatcttgc gcgggattcc cgcgattgca
    14041 gtagcggttc tgctttctgc atgtagtgca aataacaccg caaagaatat gcatcctgag
    14101 acacgtgcag tgggtagtga aacatcatca ctgcaagctt ctcaggatga atttgaaaac
    14161 ctggttcgta atgtcgacgt aaaatcgcga attatggatc agtatgctga ctggaaaggc
    14221 gtacgttatc gtctgggcgg cagcactaaa aaaggtatcg attgttctgg tttcgtacag
    14281 cgtacattcc gtgagcaatt tggcttagaa cttccacgtt cgacttacga acagcaggaa
    14341 atgggtaaat ctgtttcccg cagtaatttg cgtacgggtg atttagttct gttccgtgcc
    14401 ggttcaacgg gacgccatgt cggtatttat atcggcaaca atcagtttgt ccatgcttcc
    14461 accagcagtg gtgttattat ttccagcatg aatgaaccgt actggaagaa gcgttacaac
    14521 gaagcacgcc gggttctcag ccgcagctaa taaaccgttt ggatgcaatc ccttggctat
    14581 cctgacgagt taactgaaag cactgcttag gcagtgcttt tttgttttca ttcatcagag
    14641 aaaatgatgt ttccgcgtct tgatccaggc tatagtccgg tcattgttat cttttaaatg
    14701 ttgtcgtaat ttcaggaaat taacggaatc atgttcatac gcgctcccaa ttctggacgt
    14761 aagctcctgc ttacctgcat tgttgcaggc gtgatgattg cgatactggt gagttgcctt
    14821 caatttttag tggcctggca taagcacgaa gtcaaatacg acacactgat tatcgacgta
    14881 caaaagtatc tcgataccta ttttgccgac ctgaaatcca ctactgaccg gcttcagccg
    14941 ctgaccttag atacctgcca gcaggctaac cccgaactga ccgctcgcgc ggcgtttagc
    15001 atgaatgtcc gtacgtttgt gctggtgaaa gataaaaaaa cattctgttc atctgcgact
    15061 ggtgagatgg acattccact caatgaattg attccggcgc tcgacattaa taaaaacgtc
    15121 gatatggcga tcttacccgg cacgccgatg gtgccgaaca aacccgcaat cgtcatctgg
    15181 tatcgcaacc ctttgctgaa aaatagcggt gtgtttgccg ctctgaatct caacctgacg
    15241 ccttctctct tttatagttc acggcaggaa gattacgatg gcgtcgccct cattattggc
    15301 aatactgcgc tatctacctt ttcttcacgt ttgatgaacg ttaatgaatt aaccgacatg
    15361 ccggtccgtg aaactaaaat tgcgggcatt cctctgaccg ttcggcttta tgcggatgac
    15421 tggacatgga acgatgtgtg gtacgcattt ttactgggcg gcatgagtgg aactgtcgtt
    15481 ggcctgctct gctattacct gatgagcgta cgtatgcgcc ccggcagaga aatcatgacc
    15541 gccatcaagc gcgaacaatt ttacgtggcg tatcaaccgg tggtggatac acaagctttg
    15601 cgagtaacgg gcctggaagt actgctacgc tggcggcatc ctgtcgcggg agaaattccc
    15661 ccggatgcct tcattaactt tgccgaatcg caaaagatga ttgtgccgct gactcagcat
    15721 ctgtttgagt taattgcccg cgatgccgca gaattagaaa aagtgctgcc ggtaggcgtc
    15781 aaatttggca ttaacattgc gccggaccat ctgcacagcg aaagctttaa agcagatatc
    15841 cagaaactgc tcacttccct acccgcacac catttccaga ttgtgctgga aattaccgag
    15901 cgcgatatgc tgaaagagca agaagccaca caactcttcg cctggctgca ctcggtaggc
    15961 gtagaaattg ctattgatga cttcggcacc gggcacagcg cgcttatcta tcttgagcgt
    16021 tttacgctcg attatctgaa aattgaccgt ggatttatca acgccatcgg tacggaaacg
    16081 atcacttcac ccgtacttga cgcggtgctg acgctggcga aacgcctcaa tatgctgacg
    16141 gttgctgaag gggtcgaaac gccggaacaa gcgcgatggc taagcgaacg cggcgttaat
    16201 ttcatgcaag gctactggat tagtcgcccg ttaccgctgg acgattttgt tcgctggctg
    16261 aagaaaccgt atacgccgca gtggtaaggt gtgcttacgt cccttattat tcatagtgaa
    16321 agcatgccgg attgcggcta atgatgagta aaaggaaatc cgttgcagat gattgtgcgc
    16381 atactgctgc tgtttatcgc tctgttcacc tttggtgcgc aggcgcaggc tatcaaggaa
    16441 agctatgcct ttgccgtgct gggcgaaccc cggtacgcgt ttaatttcaa ccattttgat
    16501 tatgtgaacc ccgccgcgcc aaaaggtggg cagataacgt tgtcagccct cggtaccttc
    16561 gataatttca accgctatgc actgcgcggc aacccgggcg cacgcaccga gcagctgtac
    16621 gacacgctat ttacgacttc cgatgacgaa ccaggcagtt attacccgct gattgctgaa
    16681 agcgcacgct atgctgacga ttattcctgg gtggaggtcg ctattaatcc gcgcgcccgt
    16741 tttcatgatg gttcgcccat tactgcccgc gatgtagagt ttacttttca aaaatttatg
    16801 accgaaggcg tgccgcaatt tcgtctggtc tacaaaggca ccaccgtcaa agccattgcg
    16861 ccgttaaccg tgcgcattga gttagctaaa cccggcaaag aagatatgtt gagtctgttt
    16921 tcgctgccgg tatttccaga aaagtactgg aaggatcaca aacttagcga tccgctcgcc
    16981 acgcctccgc ttgccagtgg tccgtaccga attacgtcct ggaaaatggg gcaaaatatt
    17041 gtctattccc gcgtgaaaga ttactgggca gcaaacttac cggtaaaccg tggacgctgg
    17101 aatttcgaca ccattcgcta cgattattac ctcgatgata atgtcgcctt tgaagcgttt
    17161 aaagcaggtg cctttgattt gcgtatggaa aacgacgcta aaaactgggc cacgcgttat
    17221 accggtaaaa atttcgataa aaaatacatc atcaaagatg agcaaaagaa cgaatcagcc
    17281 caggatacgc gctggctggc gtttaatatc caacgtccgg tattcagcga tcgccgggtc
    17341 cgggaagcaa tcaccctcgc ctttgacttt gaatggatga acaaggcgtt gttttacaat
    17401 gcctggagtc gcacgaacag ttattttcag aataccgaat acgcggccag aaattacccc
    17461 gacgccgcgg agctggtgct tctggcacca atgaaaaaag atctaccgtc agaagtcttc
    17521 acacaaatct accagccgcc ggtatctaaa ggcgatggct acgatcgtga caacctgtta
    17581 aaagccgaca aacttctcaa cgaagcgggc tgggtgctga agggtcagca acgcgttaat
    17641 gccacaacgg gtcagccact cagctttgaa ttattgcttc ccgcaagcag caatagtcag
    17701 tgggtattgc cgttccagca cagcctgcaa cggctgggta tcaacatgga cattcgcaag
    17761 gtggataact cgcaaatcac caaccgcatg cgcagtcgcg actatgacat gatgccgcgc
    17821 gtatggcggg cgatgccgtg gcccagttcc gatttacaga tttcctggtc atcggaatat
    17881 atcaattcca cttataatgc ccccggcgtg caaagtccgg ttatcgactc gctgatcaat
    17941 caaattattg ccgcgcaggg aaataaagaa aaattactgc cgttagggcg agcactggat
    18001 cgcgtattaa cgtggaatta ttacatgctg ccaatgtggt acatggcgga agaccgtctc
    18061 gcctggtggg ataaattctc ccaacccgct gtacgccctg tttacagcct gggtatcgat
    18121 acctggtggt atgacgttaa taaagcggcc aaactgccgt cagccagaca acagggagag
    18181 tagatgggcg cttatctgat tcgccgtctg ttgctggtga tcccaacgtt atgggcgatt
    18241 atcactatca actttttcat cgtgcaaatt gcgcctggcg gtccggtcga ccaggccatc
    18301 gccgccattg agtttggtaa tgccggagta ttacccggcg caggcggtga aggtattcgc
    18361 gccagccatg cgcaaacggg cgtcggcaat atcagcgaca gtaattaccg tggcggacgc
    18421 ggattagatc cagaagtgat cgccgagatc actcatcgct acggtttcga taagccgatc
    18481 cacgaacgtt acttcaaaat gctctgggac tacatccgct ttgattttgg tgatagcctt
    18541 tttcgcagcg cctcggtgct gacgctgatt aaagacagtc tgccggtttc catcaccctc
    18601 ggattgtgga gcacgctgat tatctatctg gtgtcgattc cgttaggcat tcgcaaagct
    18661 gtttataatg ggagccgctt tgacgtctgg agtagcgcat ttatcatcat cggctacgcc
    18721 attccggcct ttttgtttgc catcctgctg attgtcttct tcgcgggcgg cagctatttc
    18781 gacctgttcc ctctacgcgg cctggtttcc gctaactttg attcgctgcc gtggtatcag
    18841 aaaatcaccg attatctgtg gcatatcacg ctgccggtgc tggcgacagt gattggtggc
    18901 tttgcggcgc tgaccatgct gaccaaaaac tcattccttg atgaagtgcg caagcaatac
    18961 gtggtgaccg cgcgtgcgaa aggggtaagt gaaaaaaata ttctctggaa acatgtgttc
    19021 cgcaacgcca tgctgctggt gattgccggt tttccggcga cgtttatcag catgtttttt
    19081 accggctcgc tgctgattga agtgatgttt tcactcaatg gccttggctt actgggctac
    19141 gaagcgaccg tctcgcgcga ttatcctgta atgtttggta ccttgtatat tttcaccctg
    19201 attggcctgc tgctgaatat tgtcagtgat atcagctata cgctggtcga tccgcgtata
    19261 gattttgagg gacgttaatg tcgcgactca gccccgtcaa tcaggcccgt tgggcgcgtt
    19321 ttcgccataa ccgtcgcggc tactggtcgt tatggatttt cctcgttttg tttggtttga
    19381 gtttgtgttc tgaacttatc gccaacgata aaccgttgct ggtacgttat gacggcagtt
    19441 ggtatttccc gttgttgaaa aactacagcg aaagcgattt tggcggcccg ctggcaagtc
    19501 aggctgatta tcaggacccg tggctgaaac aacggctgga aaataacggc tgggtactgt
    19561 gggcaccgat tcgctttggt gctaccagta tcaactttgc taccgataag cccttccctt
    19621 ctccaccctc ccggcaaaac tggctgggaa cggatgccaa cggcggcgat gtgctggcac
    19681 gtattctcta tggcacgcgg atctcggttc tgtttggcct gatgctgact ctctgttcca
    19741 gcgtgatggg cgtgctggcg ggggcgctac aaggctatta cggcggtaaa gtcgatctct
    19801 gggggcaacg ctttattgaa gtatggtcgg ggatgccgac gctgtttttg attattttac
    19861 tttccagcgt cgtacagcct aacttctggt ggctgctggc aattactgtc ttgtttggct
    19921 ggatgagtct ggtcggcgtg gtgcgggcgg agtttttacg tacccgtaat ttcgactaca
    19981 ttcgtgcggc gcaggcgctt ggcgtcagcg atcgcagtat catcctgcgt catatgttgc
    20041 ctaatgccat ggtcgcgacc ctcacctttt taccgtttat tttatgtagt tcgataacca
    20101 ccctgacctc actcgatttc ctcggcttcg gtctgccgct cggttcaccg tcactcggtg
    20161 aactgctgtt acaagggaaa aataaccttc aggctccgtg gcttgggatc accgtcactc
    20221 ggtgaactgc tgttacaagg gaaaaataac cttcaggctc cgtggcttgg gatcaccgcc
    20281 ttcttgtcgg tggcgatatt attgtctttg ctgatcttta ttggtgaagc cgtccgcgac
    20341 gcatttgatc ctaataaggc ggtgtagcat gacgcaaact ctgttagcga ttgaaaattt
    20401 gtcggtgggt tttcgccatc agcaaaccgt acgtacagta gtcaatgatg tttcactaca
    20461 gattgaggct ggcgaaacgc tggcgctggt gggtgagtca ggttcaggca aaagcgttac
    20521 cgcgctgtca attttacgcc tgctcccttc cccgccggtt gaatatctct ccggcgatat
    20581 tcgttttcat ggcgaatcgc tgcttcatgc cagcgatcaa acgttgcgcg gtgtacgcgg
    20641 taataagatc gccatgattt ttcaggagcc gatggtgtcg ttaaatccat tgcataccct
    20701 ggaaaaacag ctttatgaag tgctttcact ccaccgcggg atgcgtcggg aagcggctcg
    20761 aggcgaaatt cttaactgcc ttgatcgcgt tggtatccgc caggcggcaa aacggctgac
    20821 agattatccg catcagctct ccggcggcga acgccagcgg gtgatgattg cgatggcgct
    20881 gttaacgcga ccggaattat taattgccga tgaaccgacc accgcactgg acgtctctgt
    20941 ccaggcgcag attttacaac tgttgcgcga actgcaaggc gagctgaata tgggcatgct
    21001 gtttattact cataacctca gcattgtcag aaaactggcc caccgcgtgg cggtaatgca
    21061 aaacggtcgc tgtgtcgagc aaaattacgc cgctacgcta tttgcctcac ccactcatcc
    21121 ttacacacaa aagctactca acagtgaacc gtcaggcgac ccggtgccgt tgccagaacc
    21181 tgcctcaacg ttgctggatg ttgaacagct tcaggttgcc ttccccattc gcaaagggat
    21241 tttgaagcgc attgtggatc ataatgtggt ggtgaaaaac atcagtttta cgctacgagc
    21301 gggtgaaaca ctgggtttag tgggcgagtc cggttccggg aaaagtacga cgggactggc
    21361 gctgctgcga ctgattaatt ctcagggcag catcatcttt gacggtcagc cactgcaaaa
    21421 tttaaatcgc cgccagctgt tacctattcg tcatcgcatt caggtggtat ttcaggatcc
    21481 aaactcctcg ctcaacccac gactcaacgt tttgcagatt attgaggaag gcttacgagt
    21541 tcaccagccg acgctttctg ccgcacaacg cgaacaacaa gtgatagccg tgatgcatga
    21601 agtgggatta gatcctgaaa cacgccaccg ttatccggcg gagttctctg gtggtcagcg
    21661 acaacgtatt gcgattgcca gggcattaat tcttaagccc tcgctgatca tacttgatga
    21721 accgacatca tcactcgaca aaacggtaca ggcgcaaata ttgacgctat tgaaatcatt
    21781 gcaacaaaag catcaactgg cctatttgtt tatcagccac gatttgcacg ttgtccgcgc
    21841 gttatgtcat caggttatcg tactgcgaca aggggaagta gtggaacaag gaccgtgcgc
    21901 gcgcgtgttt gccacaccgc agcaggagta tacgcgtcag ctactggcgt tgagctgacg
    21961 cttaaaaagg attgtagttt gaaaagggtt cggcaattgc cacaccaaaa tttttcaatc
    22021 ggcatgttgc agcaaactcg tcctggcgat ttacaaacag gcacggctca ccttcacact
    22081 ccagcaccga acatggaact tcgatgtcgc ttaacgcctg gcttagttta tacatcaccg
    22141 accatgcatt atcaccatcc gggctaagca atttaagcgc caccaggctg ttgtcaccgc
    22201 acggtccatt ttgcggaatc ggttcaacct tcgaacctgc gaaaccagca gaccagcgat
    22261 agttctgcgg cagtcgatgg acgattgaga gttgtaatga agtcacttat tttccccgga
    22321 agtacattca cttcacaatt tgtttacatc aattttaaca catcatcaac aatccgtctt
    22381 tatacagatc gtaaagattg atgcctcgcc tgcgtggcct ctatggctct gatttaagta
    22441 atatctgcgg gcgtggtcac gtttgcgggg ctatatgtac ccgtttctgc gatctaactc
    22501 aaccttttta actacaatga tgtgactttt tacat
//
LOCUS       DAZGSP010000082        22014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZGSP010000082 DAZGSP010000000
VERSION     DAZGSP010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.082"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(5..703)
                     /locus_tag="ACHK74_003507"
     CDS             complement(5..703)
                     /locus_tag="ACHK74_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFP0222258.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            833..1774
                     /gene="rihB"
                     /locus_tag="ACHK74_003508"
     CDS             833..1774
                     /gene="rihB"
                     /locus_tag="ACHK74_003508"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFP0222259.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            1874..3124
                     /locus_tag="ACHK74_003509"
     CDS             1874..3124
                     /locus_tag="ACHK74_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP0222260.1"
                     /translation="MDVMRSVMGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAEFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWTDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(3180..4268)
                     /locus_tag="ACHK74_003510"
     CDS             complement(3180..4268)
                     /locus_tag="ACHK74_003510"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP0222261.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDET
                     VLAINDTHLLEQLSPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLAGEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(4271..5128)
                     /gene="nfo"
                     /locus_tag="ACHK74_003511"
     CDS             complement(4271..5128)
                     /gene="nfo"
                     /locus_tag="ACHK74_003511"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFP0222262.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(5202..6251)
                     /locus_tag="ACHK74_003512"
     CDS             complement(5202..6251)
                     /locus_tag="ACHK74_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFP0222263.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            6350..7231
                     /gene="yieE"
                     /locus_tag="ACHK74_003513"
     CDS             6350..7231
                     /gene="yieE"
                     /locus_tag="ACHK74_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFP0222264.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            7436..8905
                     /gene="lysP"
                     /locus_tag="ACHK74_003514"
     CDS             7436..8905
                     /gene="lysP"
                     /locus_tag="ACHK74_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFP0222265.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            9199..11190
                     /gene="cirA"
                     /locus_tag="ACHK74_003515"
     CDS             9199..11190
                     /gene="cirA"
                     /locus_tag="ACHK74_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFP0222266.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSFNYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(11222..12058)
                     /gene="yeiG"
                     /locus_tag="ACHK74_003516"
     CDS             complement(11222..12058)
                     /gene="yeiG"
                     /locus_tag="ACHK74_003516"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP0222267.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            12316..12984
                     /gene="folE"
                     /locus_tag="ACHK74_003517"
     CDS             12316..12984
                     /gene="folE"
                     /locus_tag="ACHK74_003517"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFP0222268.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            13001..14158
                     /gene="yeiB"
                     /locus_tag="ACHK74_003518"
     CDS             13001..14158
                     /gene="yeiB"
                     /locus_tag="ACHK74_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFP0222269.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLVGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            14300..15340
                     /gene="galS"
                     /locus_tag="ACHK74_003519"
     CDS             14300..15340
                     /gene="galS"
                     /locus_tag="ACHK74_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFP0222270.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSCHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            15620..16618
                     /gene="mglB"
                     /locus_tag="ACHK74_003520"
     CDS             15620..16618
                     /gene="mglB"
                     /locus_tag="ACHK74_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFP0222271.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            16679..18199
                     /gene="mglA"
                     /locus_tag="ACHK74_003521"
     CDS             16679..18199
                     /gene="mglA"
                     /locus_tag="ACHK74_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFP0222272.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            18215..19225
                     /gene="mglC"
                     /locus_tag="ACHK74_003522"
     CDS             18215..19225
                     /gene="mglC"
                     /locus_tag="ACHK74_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFP0222273.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(19296..20531)
                     /gene="preA"
                     /locus_tag="ACHK74_003523"
     CDS             complement(19296..20531)
                     /gene="preA"
                     /locus_tag="ACHK74_003523"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFP0222274.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(20525..21763)
                     /gene="preT"
                     /locus_tag="ACHK74_003524"
     CDS             complement(20525..21763)
                     /gene="preT"
                     /locus_tag="ACHK74_003524"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFP0222275.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSNVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
BASE COUNT         5299 a         5433 c         5729 g         5553 t
ORIGIN      
        1 ttttttactg catcatcccg gagaatttat tctccgggtc catctccagc gccagtcctg
       61 acaactgctt tctgttttta attagatatc ctttcttgct tttaattaat aaaccgtcgt
      121 gaatgaattg cgcgagaaca tataaaagat gtcgataaga aacgcctaaa tactctgccg
      181 cttgcgtatg cttttcgtga taaagatcac cttcttgcga gagtaatata aatgctgcca
      241 ggcgattcac taacggaaat gattgattct gagttaaaga aacaatatta cgataatttt
      301 tatgacttaa ggtgacgcaa agttttcgta aaaatagcgt gtcgtttaat aacagcggac
      361 ggtaatgttt catagggagc gcaaggcacc aacactcttc aatcgcctgt actgcacgtg
      421 gttcatggtc tttatcgatt aactcaatct cgccaataaa acagggggcg gcaaagaaat
      481 cgatcagcga cacgcgacca ttagctagcg tggcgtaaag cctggcgcgg cctcgcgtca
      541 ggtaaaacag ccaggacggc tgttgccctt cctgcacaat gtagtcacgc gctaaaaaat
      601 gaaacagccg cgtatcggct gaaacatccg ttaaaaagca atccttaaac gcggattcac
      661 tcatcaattg ctgcttaaga tcattattgt ggatttcttt cacgatgttt atccgctatg
      721 agatttctca tatttagcat acgtacgcgg ttggtaagat tcaatcatcg gcccggtcga
      781 attttctttg ctgtagaaaa aataacgcaa ctggaaacag aggaaataaa caatggaaaa
      841 gagaaaaatt attctggatt gtgatccggg tcatgatgat gctattgcaa tgatgatggc
      901 agcgaaacat ccggcaatag atttattagg catcactatt gtagcgggta atcagacgct
      961 tgataaaaca ttaattaatg gcctgaatgt ttgccagaaa ctggagatta atgttccggt
     1021 ttatgcgggg atgccgcaac ccattatgcg taaacaaatc gttgccgata atattcacgg
     1081 tgaaaccgga ctggatggcc cggtattcga gccgctgacc cgccaggcag aaagtaccca
     1141 cgcggtgaaa tacatcattg ataccctgat ggcaagcgat ggcgatatca ctctggtgcc
     1201 ggttggtccg ctttcaaata tcgcggtggc aatgcgtatg caacccgcga tcctgcccaa
     1261 aatccgtgaa attgtgctaa tgggcggcgc ttacggtaca ggcaacttca cgccatctgc
     1321 cgagttcaac atctttgctg acccggaagc agcccgcgtg gtgttcacct ctggcgttcc
     1381 attagtgatg atgggcctcg atcttaccaa ccagaccgtt tgcaccccgg acgtgattgc
     1441 tcggatggaa agggcaggcg gccctgccgg agagctgttc agcgacatca tgaacttcac
     1501 tctcaaaacg cagttcgaaa actacggcct ggctggcggc ccggtgcacg acgccacctg
     1561 catcggttat ctgattaacc ctgatggcat taaaacccag gagatgtacg tcgaagtgga
     1621 cgtcaacagt ggcccttgct atgggcgtac cgtctgcgac gagctgggcg ttcttggcaa
     1681 gcccgctaat accaaagtcg gcatcactat tgataccgac tggttctggg gcttagtcga
     1741 agagtgcgtg cgcggctaca tcaaaaccca ttaagtgttg ataacaagcc gggtcagtat
     1801 cgcaagatcc aagcccggct atttctgatg ggcagaaagc cgtcgcccaa tatgacatga
     1861 agagataata actatggatg tcatgagaag tgttatggga atggtggtat tgctgacgat
     1921 tgcgtttttg ctgtcagtaa acaagaagaa gatcagcctg cgtaccgttg gcgcggcgtt
     1981 agtgttacag gtcgtgattg gcgggattat gctttggtta ccgccagggc gttgggtcgc
     2041 tgaaaaagtc gcttttggcg tgcataaagt gatggcgtac agcgacgcgg gtagcgcatt
     2101 tatcttcggt tcgctggtcg ggccgaaaat ggacacgctg tttgatggcg cagaatttat
     2161 ctttggtttc agggtattac cggcaattat cttcgtcact gcactggtga gtattctcta
     2221 ctacatcggt gtgatgggga ttttaattcg cattctcggc ggtatattcc agaaagcatt
     2281 aaatatcagc aagattgagt cattcgtcgc ggtcaccacc attttcctcg ggcaaaacga
     2341 aattccggcg atcgtcaaac cctttatcga tcgtctgaat cgcaatgaat tatttacagc
     2401 gatttgtagt ggcatggcct cgattgctgg ttcgacaatg attggttacg ccgccctggg
     2461 cgtacctgtg gaatatttgc tggcggcatc gttaatggcg atcccaggcg ggatcttgtt
     2521 tgcccgcctg ttaagcccgg ctacggaatc ttcgcaggtt tcctttaata acctctcttt
     2581 caccgaaaca ccgccaaaaa gcattattga agccgctgcg acaggggcaa tgaccgggct
     2641 gaaaatcgct gcgggtgtgg caacagtggt gatggcattt gttgccatca ttgcgttaat
     2701 taacggtatt atcggcggcg ttggcggctg gtttggtttt gaacatgcct cgctggagtc
     2761 cattttaggt tacctgctgg ccccactggc gtgggtaatg ggtgttgact ggactgatgc
     2821 gaatcttgcc gggagtttga ttggacagaa actggcaata aatgaatttg tcgcttatct
     2881 caatttctca ccctatctgc aaacggctgg cactctggat gctaaaaccg tggcgattat
     2941 ttccttcgcg ttgtgcggtt tcgctaactt tggttctatc ggggtggtgg tgggggcgtt
     3001 ttctgcggtt gcgccacacc gtgcgccgga aatcgcccag cttggtttac gcgcgctggc
     3061 ggcggcgaca ctttctaacc tgatgagtgc caccattgcc gggttcttta ttggtttagc
     3121 gtagtgtgcg atttatgagc cggataagat gcggtcaagc gtcgaatccg gcaacattgt
     3181 tacaccattg gcactaatga aagcgcgtta tcggcagaca gggtagggtt gtttaagctg
     3241 ccgctggcgc gtgaaattgc cgcgcaggcc atcgcaaaac gggcgctgtc acggaaattg
     3301 tttccttcca gaaagctata taccaggccc gccataaaac catcgtcagc accaaaactg
     3361 tctactgtcg tatgcgctgg tgcggtcagc aaaaattgtt ctccatcctt ttcgctgcaa
     3421 taaactgact catcgggcaa ataaacaaac agttgctgaa caccttgctg atgcaacgca
     3481 ttcactgcag cattacggtc agcatcgctg gtgatcgcct gtccccataa aatttccagc
     3541 tccggtaaag tgggtttcag ggtgtgaata tgcgccagcc agtgtttgat tttgcccgct
     3601 ttgaattctg aaacggtatc gacaaacacc gggatctcac cagcgagggt aaagacccat
     3661 tccagcgcct cggctgtcag gttgcaatca gccagtacca cgcccgcatg acgaagtaaa
     3721 tcgcgcgacc cgttcaataa ttgcggcgat aactgttcca gcagatgggt atcgttaatc
     3781 gccagcacgg tttcatcgtc tcgattggca attgccagat acgtcgatgt gctttgacca
     3841 tgtaaacgaa cgcagccgga gacattcacg ccggcgcggc gcgtttcttc caggagcatt
     3901 tcgccataaa agtcatcgcc aatcactgaa agcaaatgga cgtcgcggcc taacagcgcc
     3961 agattgtggg cgatattgcg tccaacgccg cctgccgagc aatgaattgt accgggatga
     4021 gaagccgctt gcgggtaatg gatatccgcc atcccgcgaa tatccatatt gattgtcccc
     4081 accactacgc agtattcctg ctcggtgaga atgtaacctt tgcctttaat ccgccctttg
     4141 cgcattaaat ccataatatg cgccgcaacg cgcgaacgac tgatttgcag catgtccgca
     4201 atttcgttct gctgaatcag cgggttacgc cgtaaaattg caaggatctc cttttcccgg
     4261 ttattcatct tcaggctacc gctttttcag tttgttgcgc tttcagccag gcgatctctt
     4321 ctgcccagat atccgggttg atggtttcaa ggatcagcgg aatgccgtcg aaacggtcgt
     4381 cctgcatgat ccagcggaac gcatcatgac cgatattgcc ttcaccgagg ctatgatggc
     4441 ggtcaacgcg gctgccaaag gtgcttttcg catcgttaag gtgcatcccg cgcagatact
     4501 taaagccgac aatacgggca aaatcagcga acgttttctc gcattcggct ggagtacgca
     4561 aatcataccc ggcagcgaaa gcatggcagg tatcaatgca gacgccgaca cgggatttat
     4621 cttccacgcc gtcgataatc gccgcgagat gttcgaattt aaaccctaag ttactgccct
     4681 gaccggcggt gttttcaatc accgcggtca caccttgagt tttatccagc gcaatgttga
     4741 tggattcggc aatacgcgca aggcaatcct cttctgaaat ctgcatcaga tggctgccag
     4801 ggtggaagtt gagcaaagaa agccctagtt gttcgcaacg ctgcatttca tctataaagg
     4861 catcgcgcga tttttccaga gcttcagtga ccggatgtcc gaggttaatc agataactgt
     4921 cgtggggaag aatttgcgcc gatgtgtagt gatatttttc acaggcggct ttgaattcat
     4981 cgatggtttg cgtcgtgagc ggtgcggcac gccactgacg ttggtttttg gtgaacaagg
     5041 caaacgcggt tgcgtcgatt tcggcggcgc gaattgcggc atttgccaga ccgccagcag
     5101 cactaacgtg cgctccaatg tatttcatgc gaggactcct gttaaacccg ctggaggaaa
     5161 acggtaatga tagcgggtta acaggagcaa gatgtagtgg tttatgcgat gacgctttga
     5221 atcacatagt taatcgcacc accaccaaca atcagccagg caaacagtac cagtgccatc
     5281 agcagaggtt tcgccccggc ttttttcagc gcgctgacgt gagtggtcag acccagcgcc
     5341 gccatcgcca ttgccagcag gaaggtatcc agcgttacca gcatgttcac cacgctctgc
     5401 ggtaacaggt ggaacgagtt aaagatggca actacgatga acaagatggc aaaccacgga
     5461 atagtgattt tgcttttctc gccgctgttc gccccagaca gctgtttaac acgcgccgcc
     5521 agcaggatga ggaacggagc cagcatcatc acgcgcagca ttttggaaat aactgctgcg
     5581 ttttccgcat ccgggctgat ggcatgacct gccgccacca cctgcgccac ttcgtgcaca
     5641 gtagaaccaa tgtagatacc gaaagtttcc ggactaaacc attgagacat cagcggatat
     5701 atcgccgggt agaggaaaat cgcgacggtc ccgaagataa caacggttgc aacagccacg
     5761 gttactttac tggcttccgc tttcactacc ggctcagtcg ccagtaccgc ggcagcacca
     5821 cagatactgc taccggcacc gatcaaccag ctggtgtgct tatccagacc aaacactttc
     5881 tgccccagga agcaagccag caggaaggta ctggacagcg tcagcacgtc aatgatgatc
     5941 ccactgatac cgacatcggc aatttgcgag aacgtcagac ggaagccata aagaatgata
     6001 cccagacgta ataaatattg cttggcaaac agcacaccac cgtcacagct tttccagatg
     6061 tgcgggtaaa tggtgttgcc taaaaccatc cccaacaaga ttgcgagggt gagggcacta
     6121 aacccggcac ccgcaaccgc gggaatggaa ccaccccaca gggcgacccc ggtgataact
     6181 gcactcaggg ctaaccccgg aataaaatgc cacagtgtac gatgttgttt ctgtaaggtg
     6241 atattcgtca taaccctctc ctttaaccgg gcataaggtt acgactaact ggtttaaaaa
     6301 taaaattgat tatatattta taattaatct ttataagtgg taagcgacta tgcacatcac
     6361 cctccggcag ttggaagttt ttgcagaagt attgaaaagt ggatcaacca cccaggcgtc
     6421 ggtgatgctg gcgttgtcgc aatcagcagt gagcgcagcc ttgaccgacc tggaagggca
     6481 gcttggcgtt caactgtttg atcgcgtggg gaaaagactg gttgttaatg aacacgggcg
     6541 gctgctctat ccgcgagcgt tggcactgct tgaacaggcg gttgaaatcg aacaactgtt
     6601 tcgcgaagac aacggcgcga ttcgtatcta tgccagtagt accatcggta actacattct
     6661 gcctgcagtt atcgcccgtt atcgccatga ttatccgcag ttgccgattg aacttagcgt
     6721 tgggaatagc caggatgtga tgcaagcggt gctggatttc cgcgttgata ttggctttat
     6781 tgaaggaccg tgccacagca ctgaaatcat ttctgaaccg tggctggaag acgagctggt
     6841 ggttttcgcc gcgccgactt cgccgttggc ccgtggtccg gtcaccttag agcagctggc
     6901 cgctgcgccg tggatcctgc gtgaacgcgg ttccggcacg cgggagattg tcgattatct
     6961 attgctgtca catttaccga agtttgagat ggcgatggaa ttaggtaact ccgaggcaat
     7021 caaacatgcg gtgcgtcatg ggttgggaat tagttgcctg tcgcgacgtg tgattgaaga
     7081 tcaattgcag gcaggcacat taagtgaagt tgcggtccct ctcccgcgcc tgatgcgtac
     7141 gttgtggcgt atacatcatc ggcaaaaaca cctttccaac gcgctacggc gctttctgga
     7201 ctattgcgat cccgcgaatg taccgcgtta aggtgctgta caagaacatg ctggtgctgt
     7261 gtcgatttcg tgacgcagcg ccctcagcat gcattcgcca gaaaagagat tggctgcttt
     7321 acttataatc cctgggcgat catgaaggtg tcttataacc gtgtatttct gccggaagga
     7381 ttgccaatcg tctgctacaa tcgcgcctca tttttaagat ggatagcatt tttgtatggt
     7441 ttccgaaact aaaaccacag aagcgccggg cttacgccgt gaattaaagg cgcgtcacct
     7501 gacgatgatt gccattggcg gttccatcgg tacaggtctt tttgttgcct ctggcgcaac
     7561 gatttctcag gcaggtccgg gcggggcatt gctctcgtat atgctgattg gcctgatggt
     7621 ttacttcctg atgaccagtc tcggtgaact ggctgcatat atgccggttt ccggttcgtt
     7681 tgccacttac ggtcagaact atgttgaaga aggctttggc ttcgcgctgg gctggaacta
     7741 ctggtacaac tgggcggtga ctatcgccgt tgacctggtt gcagctcagc tggtcatgag
     7801 ctggtggttc ccggatacac cgggctggat ctggagtgcg ttgttcctcg gcgttatctt
     7861 cctgctgaac tacatctcag ttcgtggctt tggtgaagcg gaatactggt tctcactgat
     7921 caaagtcacg acagttattg tctttatcat cgttggcgtg ctgatgatta tcggtatctt
     7981 caaaggcgcg cagcctgcgg gctggagcaa ctggacaatc ggcgaagcgc cgtttgctgg
     8041 tggttttgcg gcgatgatcg gcgtagctat gattgtcggc ttctctttcc agggaaccga
     8101 gctgatcggt attgctgcag gcgagtccga agatccggcg aaaaacattc cacgcgcggt
     8161 acgtcaggtg ttctggcgaa tcctgttgtt ctatgtgttc gcgatcctga ttatcagcct
     8221 gattattccg tacaccgatc cgagcctgct gcgtaacgat gttaaagaca tcagcgttag
     8281 tccgttcacc ctggtgttcc agcacgcggg tctgctctct gcggcggcgg tgatgaacgc
     8341 agttattctg acggcggtgc tgtcagcggg taactccggt atgtatgcgt ctactcgtat
     8401 gctgtacacc ctggcgtgtg acggtaaagc gccgcgcatt ttcgctaaac tgtcgcgtgg
     8461 tggcgtgccg cgtaatgcgc tgtatgcgac gacggtgatt gccggtctgt gcttcctgac
     8521 ctccatgttt ggcaaccaga cggtatacct gtggctgctg aacacctccg ggatgacggg
     8581 ttttatcgcc tggctgggga ttgccattag ccactatcgc ttccgtcgcg gttacgtatt
     8641 gcagggacac gacattaacg atctgccgta ccgttcaggt ttcttcccac tggggccgat
     8701 cttcgcattc attctgtgtc tgattatcac tttgggccag aactacgaag cgttcctgaa
     8761 agataccatt gactggggcg gcgtagcggc aacgtatatt ggtatcccgc tgttcctgat
     8821 tatttggttc ggctacaagc tgattaaagg aactcacttc gtacgctaca gcgaaatgaa
     8881 gttcccgcag aacgataaga aataagtttc ctcccttcct tgctaagccc tctcaaccga
     8941 gagggctttt tcaattccat ttccctgaca aatcatgcgg atataaaatt taacatttgg
     9001 attgataatt gttatcgttt gcattatcgt tacgccacaa tcaaaaaagg ctgacaaatc
     9061 agaggctgtt ccggctttct gggatgatca cctgcataaa aaataagtcc accgcgatgc
     9121 tgccgtacgc aaggggacgt gaagaagatg tgagcgataa cccattttat tttcgtagtt
     9181 acctcatgga gatatggaat gtttaggttg aaccctttcg tacgggtcgg gctgtgtttg
     9241 tccgctattt cttgtgcatg gcctgtgtta gcggtcgatg atgatggcga aacgatggtt
     9301 gtcactgcat cttccgtgga acaaaatctt aaagatgcac cagccagtat cagcgtcatt
     9361 acccaggaag acctgcagcg aaaaccggta cagaatctga aggatgtcct caaagaagtg
     9421 cctggcgtac aactgacgaa cgaaggggat aaccgtaagg gcgttagtat tcgtggtctg
     9481 gacagcagct acaccctgat tcttgtcgac ggtaaacgcg ttaactcccg caatgccgtc
     9541 ttccgccaca atgatttcga tctgaactgg atcccggtcg attccatcga acgtattgaa
     9601 gtggttcgtg gcccgatgtc gtcgctgtac ggctccgatg cgctcggcgg tgtagtgaat
     9661 atcatcacca aaaaaatcgg tcagaaatgg tcgggtaccg ttaccgtcga taccaccatt
     9721 caggaacatc gcgatcgcgg tgatacctat aacggtcagt tctttaccag cggaccatta
     9781 attgacggcg tgctgggaat gaaagcttac ggcagcctgg caaaacgtga aaaggatgac
     9841 ccgcaaaact caacgaccac cgataccgga gaaacgccgc gtattgaagg attctccagc
     9901 cgcgacggca atgtcgaatt tgcctggaca ccgaatcaaa atcacgattt tactgccgga
     9961 tacggtttcg accgtcagga tcgtgattcc gactcgctgg acaaaaaccg cctggaacgc
    10021 cagaactact ccgtcagcca taatgggcgt tgggattacg gcaccagcga actgaaatac
    10081 tacggtgaga aagtcgagaa caaaaaccct ggcaacagca gcccgataac ttccgaaagc
    10141 aatacggtcg acggcaaata cacgttgccg ctgacggcga ttaatcagtt tctcacggtt
    10201 ggcggtgaat ggcgtcacga caaacttagc gatgcggtga acctgaccgg gggaaccagc
    10261 tccaaaacgt ctgccagcca gtacgcgctg tttgtggaag atgaatggcg gatcttcgag
    10321 ccgctggcgc tgacgaccgg cgtgcgtatg gacgatcacg aaacctacgg tgaacactgg
    10381 agtccgcgtg cctacctggt ttataacgcc accgacaccg taacggtgaa agggggctgg
    10441 gcgacggcat ttaaagcacc ttctctgttg caacttagcc ctgactggac gagcaattcc
    10501 tgccgtggcg catgtaagat tgtgggtagc ccggatctga aaccagaaac cagcgaaagt
    10561 tgggagctgg ggctttacta catgggtgaa gaaggctggc tggaaggggt tgaatccagc
    10621 gttaccgttt tccgtaacga tgtgaaagat cgtatcagca tcagccgtac gtctgacgtc
    10681 aacgctgcac cgggctacca aaactttgtc ggttttgaga cgggcgctaa cggacggcgc
    10741 ataccggtat ttagctacta caacgttaac aaagctcgta ttcagggcgt ggaaaccgaa
    10801 ctgaaaattc cgttcaacga tgaatggaaa ctgtcattca actacaccta caacgatggt
    10861 cgtgatgtca gcaacggcga aaacaaaccg ctatccgatc tgccgttcca taccgctaac
    10921 ggtacgctgg actggaaacc gctggcgctg gaagactggt cattctatgt ttctggtcac
    10981 tataccgggc agaaacgagc cgacagcgcg acggctaaaa cacctggcgg ttataccatc
    11041 tggaataccg gcgcggcctg gcaggtgact aaagacgtca aactgcgcgc aggcgtgctg
    11101 aaccttggcg acaaggatct cagccgtgat gactacagct ataacgaaga cggacgtcgt
    11161 tactttatgg cagtggatta tcgcttctga tgagaagatg cccggcgaac cgggcggact
    11221 ttcacttcag taaatactgc gcatggaagc gcaggtgatc ctctataaaa gaggcgatga
    11281 agtagtaact gtgatcatat cccggctgaa tacgcagcgt catcggccac gctttctggc
    11341 gcgcggcttc tgccagaact gcgggttgca actgatcggc aagaaactga tcattatcac
    11401 cttgatcgat aagcgtcggg atcgcatcct gcgcgttact ggcatacatc agcgcgcaac
    11461 tgtcccattc cagccatgca tttttgtcct cacctaaata gcggctaaac gctttgatcc
    11521 cccacgggac gctgcacgga ttcacaatgg gcgcaaaggc ggaaacgctg gtgtatttgc
    11581 ccgggttttt cagcgccata atcagcgcac cgtgaccacc cattgaatga ccgctaatgg
    11641 cgcagcggtc gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat
    11701 aatcatacat ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg
    11761 cgccctggcc taaatcgtag ccatcgtcgt tggcaacctg ttcgccgcgc gggctggtgt
    11821 ctggcatcac cagtacaatc cccagttccg ccgctacccg ctgggcaccc gccttggtgg
    11881 tgaagttctc gtcattgcag gttaatccgg aaagccagta cagcactggt ggtggggtgt
    11941 gatcacgagg tggtgggaga aagatactga acgtcatcgg gcagtttaag gtactggagt
    12001 cgtgtcgcca gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc
    12061 gtggctcctt gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg
    12121 agtaactttc acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc
    12181 ggcaattaca ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata
    12241 atcaggctgt aaatcagctt aataactttg cccccacgca gggcggaggc gtcacacctg
    12301 caggagaaat cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta
    12361 gttgcgcgag gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg
    12421 cgcaaaagcc ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgacctg
    12481 gctgatgaca gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt
    12541 ttctccggtc tggattacgc caacttcccg aaaatcaccc tcattgaaaa caaaatgaag
    12601 gtcgatgaaa tggtcaccgt gcgcgatatc actctgacca gtacctgtga acaccatttt
    12661 gttaccatcg atggcaaagc gacggtggct tatatcccga aagattcggt gatcggtctg
    12721 tcaaaaatta accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg
    12781 acgcagcaaa ttcttattgc gctacaaacg ctgctgggca ccaataacgt ggctgtctcg
    12841 atcgacgcgg tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg
    12901 acaacgacct ctcttggtgg attgttcaaa tccagtcaga atacgcgcca cgagtttctg
    12961 cgcgctgtgc gtcatcacaa ctgattaaaa ggcaggaacc atggagcgca acgtcacgct
    13021 cgattttgtt cgcggcgtcg ccattctggg gatcctgcta ttaaacatca gcgcctttgg
    13081 gctaccaaag gcggcttatc tcaatcccgc ctggtacggc gctattacgc cgcaggatgc
    13141 atggacctgg gcatttcttg atctcgtcgg ccaggtgaaa ttcctcacgc tttttgcgct
    13201 gctgtttggt gcgggcctgc aaatgttgct gccccgtggc agacgctgga tccagtcgcg
    13261 gttaacgctg ttagtcttgc tgggttttat tcacggttta ttgttctggg acggcgatat
    13321 tctgctggct tacgggctgg tgggcttaat ctgctggcgg ctggtgcgcg atgcgccatc
    13381 ggtaaaaagc ctgtttaata caggcgtcat gctttatctg gtggggcttg gcgttttgct
    13441 gttattgggg ctgatttccg acagccagac cagccgcgcc tggacgccgg atgcatcggc
    13501 tattttgtat gaaaaatact ggaagcttca cggcggcgtt gaagcgatca gtaatcgtgc
    13561 cgatggtgtt ggcaacagtt tactggcact gggcgcacag tatggctggc aactggcagg
    13621 gatgatgctc attggtgcgg cgctgatgcg cagcggctgg ctgaaagggc agttcagctt
    13681 acgtcactat cgtcgtactg gttttgtgct ggtggcgatt ggggtgacca ttaaccttcc
    13741 tgccatcgcc ctgcaatggc agctggactg ggcgtatcgc tggtgtgcct tcttacttca
    13801 aatgccgcgg gaactgagtg cgccgtttca ggcgattggc tatgcgtcgc tgttttatgg
    13861 cttctggccg caattgagcc gctttaagct ggtgcttgcg atcgcctgcg tcggacggat
    13921 ggcgctgacc aactatctat tgcaaacgct gatttgtacc atgctttttt accacctcgg
    13981 tttgtttatg cattttgatc gcctggagct gctggcgttt gttattccgg tatggctggc
    14041 gaatatcctc ttctctgtta tctggctgcg ttacttccgc caggggccgg tggaatggct
    14101 ctggcgtcag ttaactttgc gtgctgccgg aacggcaata tctaaaacat caagataacg
    14161 atctggatca cattcgttaa caaaacggct gtaaccgttt ccattgctgt gactcgattc
    14221 acgaagtcct gtattcagtg ctgacaaaat agccgccagc aagcagtcat ttactgcaat
    14281 ctcataacag gtagtgaata tgatcaccat tcgtgatgta gcgcgtcagg ctggcgtctc
    14341 tgtggcaacg gtttcccggg tgctcaataa cagcacgctg gtcagtgccg acacgcgtga
    14401 agcagtaatg aaagccgtga gtgagctgga ttatcggcca aatgccaatg cccaggcgct
    14461 ggcaactcag gttagcgata ccattggcgt ggtggtgatg gacgtttctg atgctttttt
    14521 cggcgcgctg gtaaaagcgg tggatctggt cgcccagcag catcagaaat acgtgctaat
    14581 cggcaatagc tatcatgaag cggaaaaaga gcgtcacgcc attgaggtgt taattcgcca
    14641 gcgttgtaat gcgttgattg ttcactcaaa agcattgagt gacgatgaac tggcgcaatt
    14701 tatggataac attcccggta tggtgttaat caaccgcgtt gtgccggggt acgcccatcg
    14761 ttgcgtttgc ctggataatc tcagcggtgc ccgaatggcg acgcgcatgt tgctgaataa
    14821 cggtcatcaa cgtattggtt atctttcttc ctgccacggc attgaagatg acgccatgcg
    14881 taaagcaggc tggatgagtg cgttgaaaga gcaggatatt attccgccgg aaagctggat
    14941 tggcactggt acgccggaca tgccgggcgg tgaggcggcg atggttgaac tgctggggcg
    15001 caatctacaa cttaccgctg tatttgctta taacgacaat atggccgctg gcgcactgac
    15061 agcattaaaa gataatggca ttgcgattcc gttacatctc tcaatcatcg gtttcgatga
    15121 tattcccatc gcccgttaca ccgacccgca attaacgacc gtgcgttatc ccattgcttc
    15181 aatggctaaa ttagccaccg aactggcctt gcagggggca gcaggcaata ttgatcctcg
    15241 tgccagccac tgttttatgc cgacgttagt gcgtcgtcat tctgtcgcaa cgcgccagaa
    15301 tgcggcggcg atcactaact caacaaatca ggcgatgtaa ccgctttcaa tctgtgagtg
    15361 atttcacagt atcttaacaa tgtgatagct atgattgcac cgttttaacg ttgtaacccg
    15421 tatgtaacag tgaataatca ctttcaccga ggtaatagcg tcataacaac aattaaagcc
    15481 gttttctgga gcgttaccgg gcatggaaga acgaatttta aaaagtgagc ttcggcgttc
    15541 agtaacactt cattaactct actgccccgc cgagcattta tctcaagcac taccctgcat
    15601 aagaaaaacc ggagatacca tgaataagaa ggtgttaacc ctgtctgctg tgatggccag
    15661 catgttattc ggtgccgctg cacacgctgc tgatactcgc attggtgtaa caatctataa
    15721 gtacgacgat aactttatgt ctgtagtgcg caaggctatt gagcaagatg cgaaagccgc
    15781 gccagatgtt cagctgctga tgaatgattc tcagaatgac cagtccaagc agaacgatca
    15841 gatcgacgta ttgctggcga aaggggtgaa ggcactggca atcaacctgg ttgacccggc
    15901 agctgcgggt acggtgattg agaaagcgcg tgggcaaaac gtgccggtgg ttttcttcaa
    15961 caaagaaccg tctcgtaagg cgctggatag ctacgacaaa gcctactacg ttggcactga
    16021 ttccaaagag tccggcatta ttcagggcga tttgattgct aaacactggg cggcgaatca
    16081 gggttgggat ctgaacaaag acggtcagat tcaattcgta ttgctgaaag gtgaaccggg
    16141 ccatccggat gcagaagcac gtaccactta cgtgattaaa gagctgaacg acaaaggaat
    16201 taaaactgaa cagttacagt tagataccgc tatgtgggat accgctcagg cgaaagataa
    16261 gatggacgcc tggctgtctg gcccgaacgc caacaaaatc gaagtggtta tcgccaacaa
    16321 cgatgcgatg gcaatgggcg cggtagaagc actgaaagca cacaacaagt ccagcattcc
    16381 ggtgtttggc gtcgatgcgc tgccagaagc gctggcgctg gtgaaatccg gtgcactggc
    16441 gggcacagta ctgaacgatg ctaacaacca ggcgaaagcg acctttgatc tggcgaaaaa
    16501 cctggccgat ggcaaaggtg cggctgatgg caccaactgg aaaatcgaca acaaagtggt
    16561 ccgcgtacct tatgttggcg tagataaaga caacctggct gaattcagca agaaataaga
    16621 ctgattgggc gcagtcgttt actgcgccct tattgtatgg ccaacaaggt aaaattatat
    16681 ggtcagctca acgactccgt cttccgggga atacttgttg gaaatgagcg gtatcaacaa
    16741 gtcctttcct ggtgttaagg cacttgataa cgttaattta aaagtccggc cacattctat
    16801 ccatgcatta atgggggaaa acggtgcagg aaaatcgaca ttattaaaat gcctgtttgg
    16861 tatttatcaa aaagactccg gcaccatttt attccagggt aaagagatcg atttccattc
    16921 cgccaaagag gcactggaaa atggtatttc gatggtacac caggagttaa acctggtatt
    16981 acaacgttcg gtgatggata acatgtggct ggggcgatat cccaccaaag gcatgtttgt
    17041 cgatcaggac aaaatgtacc gcgaaaccaa agcgattttt gatgaactgg atattgatat
    17101 cgatccgcgt gcgcgcgtcg gcacattatc cgtttcgcaa atgcagatga tcgaaatcgc
    17161 caaagcgttt tcctataacg cgaaaattgt gattatggat gaaccgactt cttcgttaac
    17221 cgaaaaagag gtcaatcatc tgttcactat tattcgtaaa ttaaaagagc gcggctgcgg
    17281 tattgtttat atctcgcata aaatggaaga aatcttccag ttatgtgatg aagttaccgt
    17341 attgcgcgac ggtcagtgga tcgccaccga accgctggca ggactgacga tggacaagat
    17401 catcgccatg atggttgggc gttctcttaa tcagcgtttt cctgacaaag aaaacaagcc
    17461 gggcgaagtc atcctcgagg tgcgtaacct aacgtcactg cgccagccgt cgattcgcga
    17521 tgtctcgttt gatctgcata aaggggagat cctcggtatt gccgggctgg tgggggcgaa
    17581 acgtaccgat attgttgaga cgttatttgg tattcgcgag aaatcggctg gcaccattac
    17641 gttgcacggc aaacagatca ataaccataa tgccaacgaa gccataaacc acggatttgc
    17701 actggtaact gaggagcgcc gctcaacggg aatttatgcc tatctggata ttggttttaa
    17761 ctcgttaatt tccaatattc gcaactacaa aaataaagtt ggtttactgg ataactcgcg
    17821 gatgaaaagc gatacccagt gggtgattga ttcgatgcgg gtaaaaacgc cgggtcatcg
    17881 gacgcaaatt ggttcgctct ccggtggtaa tcagcaaaag gtgattattg gtcgctggct
    17941 actaacgcaa ccagaaatat taatgctcga tgaaccgacg cgcggtattg atgtcggggc
    18001 gaagtttgaa atttatcagt taattgccga actggcgaag aaaggcaagg ggattattat
    18061 tatctcctct gaaatgcctg agttgttagg gataacagac cgtattctgg tcatgagcaa
    18121 tggtctcgtt tccggaattg tcgatacaaa aacaacaacg caaaacgaaa ttctgcgtct
    18181 tgcgtctttg cacctttaag attaggggct tcccatgagt gcgttaaata agaaaagttt
    18241 tcttacttac ctgaaagagg gcggtattta cgtcgttctt ttagttttgc tggcgattat
    18301 tattttccag gacccaacat ttttaagtct gttgaactta agtaatattc tcacccagtc
    18361 atcggtgcgt attattatcg cgctcggtgt ggcagggtta attgtcaccc aggggaccga
    18421 tctttctgct ggtcgtcagg tagggctggc ggcagtggtg gctgcgacat tattgcagtc
    18481 catggataac gccaacaaag tgttcccgga aatggcgacg atgccgattg cgctggttat
    18541 tctgattgtc tgtgccattg gtgcggtgat cggtttgatc aacggtctga ttatcgctta
    18601 tctcaacgtg acgccgttca ttaccacgct cggcacgatg atcatcgtct atggcatcaa
    18661 ctcgctctat tacgactttg tcggggcgtc gccaatttct ggttttgaca gtggcttctc
    18721 tacctttgct cagggctttg tcgcgctggg gagtttccgt ctctcttaca tcaccttcta
    18781 cgcgttgatt gcggtggcgt tcgtctgggt gttgtggaac aaaacccgct tcggtaagaa
    18841 catttttgcc attggcggta acccggaagc ggcaaaagta tctggtgtca acgtcggcct
    18901 gaacctgctg atgatctacg cgttgtctgg cgtgttctat gcctttggcg ggatgttaga
    18961 agccggacgt atcggctctg ccaccaacaa cctcggcttt atgtatgagc tggatgctat
    19021 cgcggcgtgc gtggtaggcg gtgtatcgtt cagcggcggt gtggggacgg tgattggcgt
    19081 ggtgaccggg gtaattattt ttaccgtcat caactatggc ctgacgtata tcggcgtaaa
    19141 cccatactgg cagtacatca tcaaaggggc gattattatc ttcgccgtag cgctggattc
    19201 actgaaatac gcgcgtaaga agtgatgtga ttgtggatat tgccggatgc gctgcatccg
    19261 gcaatcggtg ccaaatgccg gatgcgtctc tggttttaca acgttaccgg gtgttctttc
    19321 tcgcctttct taaacttcac ttccccgagc tcaatacaac ccaccgggca gacgtgacca
    19381 caaagcagac aacccacaca tttctcggta ttacaatgcg gtgtgcgggt tttctcgctc
    19441 cattccatcg cctggtgacc gccgtcgtaa caggaaatat aacagcgtcc acagccaaca
    19501 catttatcaa gattgatacg gggatagaca atataactgc ggtctaaatc ttccgccggg
    19561 acaatattgt tattcgccag acctaccatc tcctgcagcg aatcaaatcc ctgatcggcg
    19621 agataatgcg acaacccgct tgccatatct tccactatcc gatacccgta ctgcatgatg
    19681 ccggtggtca cctgtaacgt tgctgcgccg agcaataaaa actcagccgc atcctcccag
    19741 gtttcaatgc cgccgatacc gctgattggg aaatcgcgca gttctggatg ggtgcgcatt
    19801 tgctgaatga agcgcagggc gatcggtttt accgctttac cggaatatcc ggaaatactc
    19861 gattttccgt taacgatcgg cataccgatt ttctgattaa gatcgatatt ggtgatggat
    19921 ttaacggtgt taatcgccgc aatgccatct gcgccgccgc gcttcgccgc cagcgccact
    19981 tcgcacatat caccgatatt cggcgtcatc ttcgccagca ttggcagcgt ggaaccccgt
    20041 ttcactgccc gacaatattt ttctaccagc tccgggcttt gcccgacatc gctacccatc
    20101 gcatgagaag tcatttgcgg acaggagaag ttacactcga tcatatccgc gccagcttct
    20161 tgcaccaggc gcgccagctc ctcccattgc tgctcatttt cccccatgat cgaagcgatc
    20221 aatactttgt ccgggtaatc ttccttcagc tgacgcaggg cggccagatt ctcttccaac
    20281 ggatgttcag caatctgctc catatttttg aagccgataa aaccggtatc ttctttcacc
    20341 agatgatcaa aacgcggcga gacttcgttg gcgataaaaa agccgatcgt tttaaacacc
    20401 acgccgcccc aacctgtgtc gtaggctttg gcacacatct catagcagtt gcctacaggc
    20461 gaagaagaga ggcagaacgg gttgggaaac ttcacgccgc aaaaagtaat cgaaagatct
    20521 ttcgttaaca tgagcaagct ccctctaaat agtgatgaat cgcctcggcg gcttctttcc
    20581 cggttttcac ggcatagacc acggttttgt caccctcaac aatatcgcca gcagcaaaga
    20641 cttgcggatc gcgggtctga taattttgtg ttttgatggt gttacgctgc ggctctaact
    20701 ccgcaaaggc atccagtctg gcatgctgac cgacggcgag aataatttta tctgccgcca
    20761 tcgtcagttc gcccggtaac cgtacatgct taaacgtgac tttattgcct tccacggcta
    20821 ctggcgtgaa tccatcgatg atcgaaacgc ccagctcccg ggcgctggca aactcttttt
    20881 cgcttgccgg aaactcatct aactcttcac gcgctacgca ggttaccgcc tgacagccga
    20941 gaactttcag cgtgctggct acgtccatcg caacatcacc gccgccgata attaatgcgc
    21001 tttgtggaat gctgatatcg ccttgcgcct gtcgtgcacg ttgcaggaag tccacggcaa
    21061 tctcaacgtt gctatgctca aacagcgata acccggaacc gctcgataac cccacggtga
    21121 ccagtaccgc gcggttttct gctttcagct gctcaagggt gagtgtgtta ccgacttcgt
    21181 tgttgcactt aatggtcacg cccatttttt caatacgggc gatctctgca tccagcactg
    21241 attgcggtaa ccggaattgc ggaataccgt tacgcaacca gccaccgggg tgcgcttctt
    21301 tctcataaat cgtgacgtca taaccctggt ttgtcagtgt tacactggcc tgcaatccgg
    21361 caggacctgc gccaataatc gcgactttgc cgagcgtttt agtaccgggc tgataaattt
    21421 ccatcccagt ttgttgttca aaatcagtaa caaaacgctg taagcgaccg atatcaatgg
    21481 gagtatcaac accggcacgg gtacaaccgc tttgacataa tttctccgtc gggcacactc
    21541 tggcacaaac ggcaccgagg gcattatttt cgcgaattgt ctcggcagcg cctttaaaat
    21601 tacgaaagta gattgagcga ataaatttcc ccggatcggt ctgtgccggg caagcctgac
    21661 tacagggagc gtcatgacat aataaacagc gagaggcttc tttaatcgcc agtaaaggcg
    21721 taaaagccgg agtgagttca tccagataat tttgttgcgg catgtgatct tccttaatgc
    21781 tatgagtaat taaggcgaac gctaaatata ctcgtcatac ttcaagttgc atgtgctgcg
    21841 tctgcgttcg ctcaccccag tcacttactt atgtaagctc ctggggactc actcacttgt
    21901 cgccttcctg caactcgaat tatttagagt ataaatattg cgtgctgata ttaaggctat
    21961 gcgcgtgggg aaatttatac tgtgaaagga ttcacaaata gctgcggttc agaa
//
LOCUS       DAZGSP010000083        21703 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZGSP010000083 DAZGSP010000000
VERSION     DAZGSP010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.083"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(161..2194)
                     /gene="betT"
                     /locus_tag="ACHK74_003525"
     CDS             complement(161..2194)
                     /gene="betT"
                     /locus_tag="ACHK74_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFP0222276.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEVARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2323..2910
                     /gene="betI"
                     /locus_tag="ACHK74_003526"
     CDS             2323..2910
                     /gene="betI"
                     /locus_tag="ACHK74_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFP0222277.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2924..4396
                     /gene="betB"
                     /locus_tag="ACHK74_003527"
     CDS             2924..4396
                     /gene="betB"
                     /locus_tag="ACHK74_003527"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFP0222278.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETLFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4410..6080
                     /gene="betA"
                     /locus_tag="ACHK74_003528"
     CDS             4410..6080
                     /gene="betA"
                     /locus_tag="ACHK74_003528"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFP0222279.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            6293..6958
                     /gene="ykgH"
                     /locus_tag="ACHK74_003529"
     CDS             6293..6958
                     /gene="ykgH"
                     /locus_tag="ACHK74_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFP0222280.1"
                     /translation="MREQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLFFLYINK
                     DNIKVSYSLKINQTTPGILVNCDSNNNFAGQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAETEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSG"
     gene            complement(7204..7899)
                     /gene="ykgG"
                     /locus_tag="ACHK74_003530"
     CDS             complement(7204..7899)
                     /gene="ykgG"
                     /locus_tag="ACHK74_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFP0222281.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(7892..9319)
                     /gene="ykgF"
                     /locus_tag="ACHK74_003531"
     CDS             complement(7892..9319)
                     /gene="ykgF"
                     /locus_tag="ACHK74_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFP0222282.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(9330..10049)
                     /gene="ykgE"
                     /locus_tag="ACHK74_003532"
     CDS             complement(9330..10049)
                     /gene="ykgE"
                     /locus_tag="ACHK74_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFP0222283.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVSASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(10576..11430)
                     /gene="rclR"
                     /locus_tag="ACHK74_003533"
     CDS             complement(10576..11430)
                     /gene="rclR"
                     /locus_tag="ACHK74_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFP0222284.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            11656..12981
                     /gene="rclA"
                     /locus_tag="ACHK74_003534"
     CDS             11656..12981
                     /gene="rclA"
                     /locus_tag="ACHK74_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFP0222285.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHNENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            13090..13326
                     /gene="rclB"
                     /locus_tag="ACHK74_003535"
     CDS             13090..13326
                     /gene="rclB"
                     /locus_tag="ACHK74_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFP0222286.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNKSTATAVLYK"
     gene            13338..13931
                     /gene="rclC"
                     /locus_tag="ACHK74_003536"
     CDS             13338..13931
                     /gene="rclC"
                     /locus_tag="ACHK74_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFP0222287.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(14091..14960)
                     /locus_tag="ACHK74_003537"
     CDS             complement(14091..14960)
                     /locus_tag="ACHK74_003537"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP0222288.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            15209..16066
                     /locus_tag="ACHK74_003538"
     CDS             15209..16066
                     /locus_tag="ACHK74_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0222289.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            complement(16187..20440)
                     /gene="fdeC"
                     /locus_tag="ACHK74_003539"
     CDS             complement(16187..20440)
                     /gene="fdeC"
                     /locus_tag="ACHK74_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFP0222290.1"
                     /translation="MSRYKTGHKQPLFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     AHAQHAVQPRLSMENTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYHKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDTDGNPVTGEASRLR
                     FVPQDTNGVTIGTISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLIAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGMKPDAPVFSGAASTGTERPSTGDWTETSNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVDNQLANGQSTNQVTLTVVDTYGNPL
                     QGQNVTLTLPKGVTSKTGNTVTTDAAGKADIELMSTVAGEHSITASVNNAQKTVTVKF
                     KADFSTGQASLEVDSAAPKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANGEGKAELQVVSVTAGTYEITASAGNSQPSDTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
     gene            20993..21082
                     /locus_tag="ACHK74_003540"
                     /pseudo
     CDS             20993..21082
                     /locus_tag="ACHK74_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21556..21657
                     /gene="ykgR"
                     /locus_tag="ACHK74_003541"
     CDS             21556..21657
                     /gene="ykgR"
                     /locus_tag="ACHK74_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFP0222291.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFFHFY"
BASE COUNT         5618 a         5552 c         5050 g         5483 t
ORIGIN      
        1 aataaaatag ttaattaata aaatataaaa tcatgaagtg atacaagagg aattgcagga
       61 tatgacatga caatcatata taatatatgc ataatttttt acagcgagtc aatgattccc
      121 gcgccatatg cagacatggc gcggttttat gcaaaaacaa tcacgcgtcc gggaacatca
      181 cgctatggcc cggcgcttca cgatggagat gaataaagtt aaggtgccgc tcgtactggt
      241 caagaatatc ggtgatcacc tgctctttgc tgtagtccat caggtcgttg ccctggctgc
      301 cttctaacag gaaggtttcc agccggtagt aggtcgattt accgctacgt gcgcggtagg
      361 taaagcccgg caccgaatat tgctgcggcc aaatctgata gacaaagttt tgctcttcgc
      421 ccatatgcac caacaaatcc agatgcccca actgttgtcc ctcttccggc ggcaggcttt
      481 ttagctccac gtacgcgccg cgcaaccgca gctcctgcgc cacttcttcc attgccgggt
      541 aacagaccgt ctccatcatc tgtttagtgt aacgcgtgcc cggataattc atcaggcgcg
      601 agagacgttt tttccagctc aggcgatcct gaagccccag cggtcgcggc gcggtatcgc
      661 ggttggcact ttcacggcgg taatcttcta ccttcagaga tttatacaac cccgccatca
      721 cgaagaaaat cacaaagctg aacggcagcc ccatgattac cgtggtgttt tgcagcgcgg
      781 atatcccgtt ggtcatcagc atgccgagcg tcagcagacc aatcgccacc gaccagaaga
      841 cgcgcagcca gccgggggcg tcgctgttga tatctttaag ctgcgaggtg aaattcccca
      901 gcaccagcgc accggagtcc gccgaggtca cataaaacag caggccagta atggtggcga
      961 cggaggcgct aaaggtaaac gccggatact gcgccagcag gctgtagaag ccgcgctccg
     1021 gatggaccat cgcttcctcg gcaaatgccg cgccgccgtg gatgatttca tacagcgcgc
     1081 tattgccgaa caccgagagc cataacagcg tgaaggtaaa cggaataatc aacgtgccca
     1141 gcacgaactg gcgaatggta cgcccacgcg agatacgcgc caggaacaag ccgacaaacg
     1201 gcgaccatgc cacccaccat gcccagaaga agagcgtcca gttattcatc cactcaactg
     1261 gacggtcgaa ggcaaaactg ttgagcgtca tgcccataaa gcgattcaca tagtcgccaa
     1321 cattcagcac cagcgcatta agcaggaacg aggtgtcgcc cataaacaat acgaacagga
     1381 tcaatcccag cgccagcgca acgttaagtt ccgataacac acgaatcccc ttatcgacac
     1441 cggaggtgac agagatcgtg gcgattatca ccgacaaggc gatcagtgcc gcttttgccg
     1501 ccatcgaatc gggaatatca aacagtacgc tcaagccata gttaagctgc accacaccga
     1561 taccgagcgt agtggcaata ccgaagatgg tgccgatcac cgctgcaata tccactgagt
     1621 gccctatcgg cccgttaatc cgtttaccga agatcgggta cagcgccgag cggatggtga
     1681 gcggcaaatt ataacgatag ctaaagtatc cgagcgccat gcccatcagc gcatacatcg
     1741 accagccggt taagccgtag tgaaacagcg tccagaccat cgcctgacgc gcgacctcaa
     1801 tcgtctgtcc cgcgccttcc ggcggctgca tatactgcgt taccggttcg gctacggaga
     1861 agaacatcag gtcgataccg atcccggcag caaacagcat cgccgcccaa ctcagcaggc
     1921 tgaattccgg tttggattgt tctggcccga gcttcaccga accaaaacgc gaacaagcga
     1981 tacagaccac aaagacaata tagagcgttg ccgccagtag atagtaccaa ccgaaggttt
     2041 tagaaaccca gtccagcgtg cggccaatcc acagggccga gaagtcgcga aacaggatcg
     2101 ttgtcaggga aaacaacaaa atcagtccgg cggaggtgta aaacaccacc ggattgattt
     2161 tgtccttttc cctgctgtgt gaaaggtctg tcatccagta tccccactgt tattgttacc
     2221 atttaaaatc aaattcgtaa caattaagac acattttata ttgaacgtcc aatcaataac
     2281 cgctttaata gataaacaac gctgatgaat ggagtggcga aaatgcccaa attggggatg
     2341 cagtcgatcc ggcgcagaca actgatcgac gccacactgg aagcaataaa tgaagtgggc
     2401 atgcacgatg caacgatcgc gcagatcgcc cgccgtgcag gcgtttctac ggggatcatc
     2461 agccactatt tcagggacaa aaatggtctg ctggaagcaa ccatgcgcga tatcaccagt
     2521 cagctgcgtg acgcggtttt gaatcgatta catgcacttc cgcagggcag tgcagagcag
     2581 cgattacagg cgattgttgg cggaaacttc gatgaaacgc aggtgagcag tgcggcgatg
     2641 aaagcctggc tggcgttctg ggccagcagt atgcatcagc cgatgctcta tcgtttacag
     2701 caggtcagca gccgccgctt gctgtcgaat ctggtgagcg agtttcgccg cgaattgccg
     2761 cgcgaacagg cacaggaagc gggctacggc cttgccgcgc tgattgacgg attatggctg
     2821 cgcgcggcac tgagcggcaa accgctggat aaacctctcg cacactcgct gacccgtcac
     2881 tttattactc agcatttacc caccgattaa ccgaggagac gtgatgtccc gaatggcaga
     2941 acagcagctt tatatacatg gtggttatac ctccgccacc agcggtcgca ccttcgagac
     3001 cattaacccg gccaacggta acgtgctggc gaccgtgcag gccgccgggc gcgaggatgt
     3061 cgatcgcgcc gtgaagagtg cccaacaggg gcaaaaaatc tgggcggcga tgaccgctat
     3121 ggaacgctcg cgtattctgc gtcgggccgt tgatattctg cgtgaacgca atgacgaact
     3181 cgcaaaactt gaaaccctcg acaccggaaa agcatattcg gaaacctcaa ccgtcgatat
     3241 cgttaccggt gcggacgtgc tggagtacta cgccgggctg atcccggctc tggaaggcag
     3301 ccagatcccg ttgcgtgaga cgttatttgt ttatacccgc cgcgaacctc tgggtgtggt
     3361 ggcggggatt ggcgcatgga actacccgat tcagattgcc ctgtggaaat ccgccccggc
     3421 gctggcggca ggcaacgcaa tgattttcaa accgagcgaa gtcaccccgc ttaccgcgtt
     3481 aaagctggct gaaatttaca gcgaagcggg cctgccggac ggcgtattta acgtgttgcc
     3541 gggggtgggc gcggagaccg ggcaatatct gaccgagcat ccgggcattg ccaaagtgtc
     3601 atttaccggc ggtgtcgcca gcggcaaaaa agtgatggct aactcggcgg cctcttccct
     3661 gaaagaagtg accatggaac tgggcggtaa atcaccgctg atcgttttcg atgatgcgga
     3721 tctcgatctc gccgccgata tcgccatgat ggcaaacttc ttcagctccg gtcaggtgtg
     3781 taccaatggc acccgcgtct tcgttccggc gaaatgcaaa gccgcatttg agcagaaaat
     3841 tctggcgcgc gttgagcgca ttcgcgcggg cgacgttttc gatccgcaaa ctaacttcgg
     3901 cccgctggtc agcttcccgc atcgcgataa cgtgctgcgc tatatcgcca aaggcaaaga
     3961 ggaaggcgcg cgcgtactgt gcggcggcga tgtactgaaa ggcgatggct tcgataacgg
     4021 cgcatgggtt gcaccgaccg tgttcaccga ttgccgcgac gaaatgacca tcgtgcgcga
     4081 agagatcttc ggtccggtga tgtcgcttct cacttatgaa tctgaggacg aagtgattcg
     4141 ccgcgccaat gataccgact acggcctggc ggcgggcatc gtgacggcgg acctgaaccg
     4201 cgcgcatcgc gtcattcatc agctggaagc gggtatttgc tggatcaaca cctggggcga
     4261 atccccggca gagatgcccg ttggcggcta caaacactcc ggcattggtc gcgagaacgg
     4321 cgtgatgacg ctccagagtt acacccaggt gaagtccatc caggttgaga tggctaaatt
     4381 ccagtccata ttctaaccag gaggtttatt tgcaatttga ctacatcatt attggtgccg
     4441 gctcagccgg caacgttctc gctacccgtc tgactgaaga tccgaatacc tccgtgctgc
     4501 tgcttgaagc gggcggcccg gactatcgct ttgacttccg cacccagatg cccgcagcgc
     4561 tggcgttccc actacagggc aaacgctaca actgggccta tgaaacagag cctgaaccgt
     4621 ttatgaataa ccgccgcatg gagtgcggac gcggtaaagg cctgggtgga tcgtcgctga
     4681 tcaacggcat gtgctacatc cgtggcaatg cgctggatct cgataactgg gcgcaagaac
     4741 ccggtctgga gaactggagt tatctcgact gcctgcccta ctaccgcaag gccgagactc
     4801 gcgatgtggg cgagaacgac taccacggtg gcgatggccc ggtgagcgtc accacctcca
     4861 aacccggcgt caatccgctg tttgaagcga tgattgaagc gggcgtgcag gcaggctacc
     4921 cgcgcacgga cgatctcaac ggctatcagc aggaaggttt tggcccgatg gatcgcaccg
     4981 tcacgccgca gggccgtcgc gccagtaccg cgcgcggtta tctcgatcag gccaaatcgc
     5041 gccctaacct gaccattcgt actcacgcta tgaccgatca catcattttt gacggcaaac
     5101 gcgcggtggg tgtcgaatgg ctggaaggcg acagcaccat cccaacccgc gcaacggcca
     5161 acaaagaagt gctgttatgt gcaggcgcga ttgcctcacc gcagatcctg caacgctccg
     5221 gcgtcggcaa cgctgaactg ctggcggagt ttgatattcc gctggtgcat gaattacccg
     5281 gcgtcggcga aaatcttcag gatcatctgg agatgtatct gcaatatgag tgcaaagaac
     5341 cggtttccct ctaccctgcc ctgcagtggt ggaaccagcc gaaaatcggt gcggagtggc
     5401 tgtttggcgg cactggcgtt ggtgccagca accactttga agcaggtgga tttattcgca
     5461 gccgtgagga atttgcgtgg ccgaatattc agtaccattt cctgccagta gcgattaact
     5521 ataacggctc gaatgcagtg aaagagcacg gtttccagtg ccacgtcggc tcaatgcgct
     5581 cgccaagccg tgggcatgtg cggattaaat cccgcgaccc gcaccagcat ccggcgattc
     5641 tgtttaacta catgtcgcac gagcaggact ggcaggagtt ccgcgacgca attcgcatca
     5701 cccgcgagat catgcatcaa ccggcgctgg atcagtatcg tggccgcgaa atcagccccg
     5761 gtgtcgaatg ccagacggat gagcagctcg atgagttcgt gcgtaaccac gccgaaaccg
     5821 cgtttcatcc gtgcggtacc tgcaaaatgg gttatgacga gatggctgtg gttgacggcg
     5881 aaggccgcgt acacgggctg gaaggcctgc gcgtggtgga tgcgtcgatt atgccgcaga
     5941 ttatcaccgg gaatttgaac gccacgacaa ttatgattgg cgagaaaata gcggatatga
     6001 ttcgtggaca ggaagcgctg ccgaggagca cggcgggata ttttgtggca aatgggatgc
     6061 cggtgagagc gaaaaaatga gtcgtgatgt gaactaacgc aggaaccgat cagattgaga
     6121 gttaccgttc cagagagggg gaccgaatcc ttatataaac actgaggtaa ctctcatgct
     6181 tcatatccag tattcatgat gctggctttt tgctgatatt taacccgaac attcctgata
     6241 cagactatta atacaacctt atctgcgaca gattttattt tctggatata ttatgcgtga
     6301 acaaatcaaa caggatatcg atctgattga gattttattt tatctgaaga aaaagattcg
     6361 cgttatcctt tttattatag ctatctgtat ggcaatggtg ctgttttttc tgtatatcaa
     6421 taaagataat ataaaagtga gttacagcct aaaaataaac cagacaacac caggcatact
     6481 tgttaactgt gatagcaata ataactttgc cggtcagacc acaatgactg aagatgttat
     6541 acagcgaatt actacatttt ttcacaccag cccagatgtc aagaacagag aaataaagct
     6601 ggaatggtca ggagataaga gagatttacc aactgctgaa acggaaatat ctcgcgtgca
     6661 ggcgtctatt atcaaatggt atgcgtcaga ataccataat ggcaggcaag ttctcgatga
     6721 gatacaaaca ccttcagcaa ttaacagtga gctttataca aaaatgatat acctgaccag
     6781 gaactggtca ttgtatccga acggtgatgg ctgtgtaact ataagctcac cagaaataaa
     6841 aaataaatac cctgctgcca tttgcctggc tctgggattt tttctaagca ttgtaatttc
     6901 tgtaatgttt tgccttgtca aaaaaatggt agatgaatac caacaaaact ctgggtagta
     6961 ataaacagca ttctgataga gagtgcctgt ataaagctga agtgacaatg accgggaagg
     7021 gaacccgttt tcaggaatgg atgcctgtct ttttcactgt aaatatcctg gcattactaa
     7081 tgccaggata tttacagaag agcagcataa ccatgacgga acggggttaa gtcaggttgt
     7141 ccttaacgct attttcaacc tcgtatgcct tcttcaggtt tatgttcaga tctcatatct
     7201 ctctcagcaa tcctcaataa tcagatacac cgctttcacc ggaccatgaa ccccgacgac
     7261 tttgataagc tcaatatccg ccgttgaact agggccgcta atgatgttaa tgcaggacgg
     7321 cattcgttca ccggcctgcg ctttctgatg caatttttct gcgagttgcg ctacacgcgg
     7381 caagatagtg cttttacgca ggataaaaag agaagattcc gggagcaggc tcaatgaacg
     7441 cccgcgcccg gcggcggaaa aaagaaccac gcctcccgat tcggttaaac catattcagc
     7501 atacacgaca cccactttag cctgctcggc ctgcgagata ttctcggcac ctttcgccgg
     7561 atcccaaaca acggcattgc attcctgctg caaacgttca ctaatcccca attcctccag
     7621 cctcgtgtca ccgctaatca cgaccgactg atctcccagc tctttacaca gacgtattgc
     7681 agcttctgcc gccttcgcct cgctggtcag ctcacagcgc gtcaacataa catcgctggc
     7741 aaactgaata aacgcgtcac agcgctgctg ttggttaagt tgggtaagcc gctcgttagc
     7801 atagttgtta agcggcgcat cttctgcttg cggttcaagt cgcagcgggc gacccagtgc
     7861 ctgagcaacg ttattcaaaa attcgcctcg attatccatt ctttttctcc tgcgcctgat
     7921 gtttcttaaa ccaactacgg aaactctctc cgtcagcttc aggaagatcg cgtgcttcca
     7981 tccagtcgct aatcgcgcca aatttgagtg gtgttttgcc gccattgata aaccagcttg
     8041 ccgcatgagc gccggccatc atcccgactt tccacaatcc tggatgacta ttggcataag
     8101 cgaacatttt tatcgcccgt tgctctgctt ttgcggtgat ccctttttca gccatcaccc
     8161 gacgatgacg caaaatcagt tttgacagcg gaatacgcac cggacacacg ctgtcacaag
     8221 cagtgcataa agagcaggcg tagggtaaat ctttaaaatc tttatagccg ccaagtagcg
     8281 gagaaatcac cgcaccaatt ggccctggat aaatagagcc atatccatga ccgccaatat
     8341 ggcgatatgc cggacaagta ttcatacaag ccccgcagcg aatacagcgc agcacatccc
     8401 gaaattcaga ggccagcacc tcagaacgcc cgttatcgac aataaccaga tgaaactctt
     8461 caggaccatc aacgtgcccc gcttcgcgcg gtcctgtcag ccaggtgttg tatcccgtca
     8521 aacgtgcacc aacggcactg cgcgccagca tggtgatcaa tacatctacc tcggcaaacg
     8581 tgggggcaat acgctccatt cccatcactg caatatgcgt tttaggcagc gtggtacaca
     8641 ttcgcgcatt accttcattg gtcaccaggc ataccgaacc tgtctctgcc accgcgaaat
     8701 tacagccggt aatacctatt tcagcactga ggaaatcttc gcggattttt tgccggatga
     8761 ataaggtcat cgcttcaggc gtttccggcc cctcatagcc cagacgttca tgtagcactc
     8821 gacggatctg atggcgatct ttatgaattg ccgggaccac aacatgagat ggcggatctt
     8881 gatccagctg gagaatatat tcacccagat cggtttcaat cacctgaatg ccagcatcct
     8941 gcaacacatg attgacacca atctcttcgg tcaccatcga tttagatttc accaccttcc
     9001 gggcattttt gcgttgggca acctgtaaaa tgtagcgggt agcgtcttct ttggtttttg
     9061 caaaccagac gtgaccgccg ttttgcgtca ctttttctga gagctggtac agataagcgt
     9121 cgagattact cagaacatga tcacgtatct gggcggcccg atcgcgccac tcctcccagt
     9181 gccccaattc atcgaccatt ttttgccgat ttgccccaat acgctgctgc gcgtttgcca
     9241 ccgctttgcg catgatcgga tcttcaattt gctgacggat gcgtgtctta aaatctgtat
     9301 tactggtttt gatcgacatc tttatatcct cagcggctca tcaacacttc agcaatatgc
     9361 atcactttga ctttctgccc ttcccgttgt aatcgcccac tgatgttcag caggcaactc
     9421 acgtcagcac caattaaata ctcagggcgg acttccatca ggtgcacaac cttttctttc
     9481 accatctcgc cggatatttc ggccattttg accgagaacg tgccgccaaa tccgcagcag
     9541 gtatcctgtt cagcaaaggt caacagctcc agtccacgca catttttcag cagcgtaagt
     9601 ggctcgtcct tcactcccag cttacgggcc aggctacaag atgggtgata cacagctctc
     9661 ccttgcaaac tggcacttac atcgactacc cctaatttat taacaataaa agaggtgaga
     9721 tcctgcatac gcgcggcaac cttttcggca cgtgatgccc attcaggttc atccgccaga
     9781 tacgtcgggt aactttttac ggcataggtg caagagccag ccggtgaaat aatgggatcg
     9841 tcgttatcct ccagtgcggc gatcagattt ttcatccctg gaatcgcttc tttgatataa
     9901 ccgctattga tcgcaggctg accgcagcat ccctgtttct ccgggaaatt tacgcgacag
     9961 ccgagttttt ccagtagcag cacggagtct cgtgccattc ttgatttcag ggcgtcacca
    10021 atacaggtga caaagaaatt gacattcaca actaatactc cattacttca tgcccattta
    10081 tgcccgatgt ttccccccca ccgggggatc aatcatcaat aaatatggcg ttctttttta
    10141 attggctagc aacataccac ccctcatttg tatgacaaat gataatcttt catccaaact
    10201 tcaaatgcaa taactaattt tatttctttc ggcatcgctc acattttatg gggaaatcga
    10261 cgttcatttt tggcataaga aatccattta aaaaggctac atcccttata cctattcaga
    10321 gctacttatt caggggttat tgagatatac actgatggtg tacaaaaaaa tcaccgacaa
    10381 taatcacgta tcaagtaata gtcggggttt tatttaattg aagcaattta gtttgcattt
    10441 ttatttaaaa actgagctag agtccatcag aaaaaaatat ataaagccca aaccaccgtt
    10501 ggaaatagaa atagttgaaa ggctcacaac tcccggaatg cggattatct ttgccctgat
    10561 ttctggcggt tttattcagg gtgcaagctg tctgaccctt tcccgatatt ctcccggagt
    10621 acaaccaaac tcgcggacaa acgccttgtg aaaagatgat tcactggcat agcctactga
    10681 ctcagcgatc accacaacag ggagcatttc ccgggaaaac atctgggccg ctatttgtag
    10741 acgcaacttt gttaatacag ccagcggcgt ggttccggaa acatcacgga aaagctgggc
    10801 aaaacttgcc cgggacatgt gagcgatgct ggccagcgat tcgacggtcc aggcgtgtcc
    10861 tggcatttcc agcatttgct gtattaccgc accaagacgt ggatgcagaa gcaaactgag
    10921 aatgtttttc tcagtattaa cctgtgcaat ccactcacgc accgcgaggg taaagaatgt
    10981 agcgcagatc tggctacaca gtgcatccac tcccggcatc gccgatctgg attcctgttg
    11041 taaaaacggg atcgcctccc tcagccagtt atattccacg ctgtggttaa ccggtgctaa
    11101 aaacagcgtt tccggcaaag acgttaaaaa ataacgcgcc gaatgttgca accgaagagt
    11161 gccgcagaca atacaggtcg attcattatc gacatgactc aggcgatgag ctgaattttg
    11221 tggtagcagg accacatttc ccgggcgtaa tgtaaaaatc tctcccgtcg gcatttccag
    11281 cttcgccgct ccttgcgtta acgcatgcca acgaataacc gataattccc cggcaccatg
    11341 tggaagctgc cagtcacttc ctaacacgca attcttatcg atcgttcctt gtggagcgtt
    11401 aagcatcaac aaacggctaa gggcatccat atcagactcc tgagtggttt ttgattacaa
    11461 acaccaacaa tcttagtaaa taactaatct aataccatca aatggttaat catgattttt
    11521 gttttgtgat tctgattaag aaaaagaaaa taccagacga taagacaaaa tatcaagcgt
    11581 tgtgaagaaa tggtatttgc tcttttgcgt ctatagtcat gatgtcaaat gaacgcgttt
    11641 cgacaggaaa tcatcatgaa taaatatcag gcagttatta ttggttttgg caaggctgga
    11701 aaaacattag ccgtcacgct ggcaaaagca ggttggcgag tggctctcat cgaacaatca
    11761 aatgcaatgt atggcgggac ctgtattaat atcggctgca tcccaaccaa aacattggtt
    11821 catgacgcac agcagcacac agattttgtc cgtgccatac agcgtaaaaa tgaagtggtt
    11881 aattttttac gtaataagaa ttttcataat cttgcggata tgcccaatat cgacgtgatc
    11941 gacggccagg cggagtttat caataatcat agcctgcgtg ttcatcggcc tgagggaaat
    12001 ctggaaattc atggcgagaa aatttttatt aataccggtg cacaaaccgt ggttccgcca
    12061 attcctggaa ttaccaccac gcctggtgta tatgacagca ccggattact taatctaaaa
    12121 gaattgcctg ggcatttagg tattttgggc ggcggatata ttggcgttga gttcgcctct
    12181 atgttcgcta attttggcag caaggtaacc attttagaag cagcttcgct gtttttgcct
    12241 cgggaagatc gggatattgc tgataatatc gcgacgattt tacgcgatca gggcgtcgat
    12301 attatcctca atgcccatgt ggagcgaatc agtcacaatg aaaatcaagt gcaagtgcat
    12361 agcgagcacg cccaactggc ggtggatgca ctgttaatag cttccggtcg tcaaccggct
    12421 accgcttcgt tacatccaga aaatgccggt atcgcagtaa acgagcgcgg ggcaattgtc
    12481 gttgacaagc aattacatac caccgcagac aatatttggg cgatgggaga tgttaccggc
    12541 gggctgcaat ttacttatat ttcactggat gattaccgca ttgtgcgtga tgagttgctg
    12601 ggagaaggca gacgtagtac tgatgatcgg aaaaatgtgc cttattccgt attcatgaca
    12661 ccgccactgt ccagggttgg tatgacagaa gaacaggcca gagagagtgg tgctgatatt
    12721 caggtggtga cattgcctgt agctgcaatt ccgcgtgcca gagtgatgaa tgatactcgt
    12781 ggggtattaa aagcgattgt tgataataaa acccaacgta tattaggggc atcactgctg
    12841 tgtgttgact cccacgagat gatcaatata gtgaaaatgg tgatggatgc cgggctgcct
    12901 tatagcatat tacgcgatca gatatttact catccgtcga tgagcgaatc actcaatgat
    12961 ctattttcat tagtcaaata aactcaaaat cagacgccag aacaaatatt ctggcgtctc
    13021 agagaaaaga atcttattaa ttccctgtta ctctataact tcttaatcac ttcattgatg
    13081 gtattttata tgtttaaaaa atctgtttta tttgcaacac tattatctgg cgttatggca
    13141 ttttccacca atgcagatga taaaataatt ctgaaacata tcagcgtctc gtcagtatca
    13201 gcatcaccga cagttctgga ggataccatt gctgatatag ccagaaaata taatgcttca
    13261 tcctggaaag tcacatcgat gcgaattgat aataaatcaa ccgcaacagc agtattgtat
    13321 aaataaggat gttcacaatg gaaaaatacc tgcacctgtt aagtcgggga gataaaattg
    13381 gcctgacatt gattcgtctg agtattgcaa ttgtttttat gtggattggg ttattaaagt
    13441 ttgtccctta cgaggcagac agcattacac cattcgtcgc aaacagtcca ctaatgtcgt
    13501 tcttttatga acacccggaa gactataaac agtatctgac tcacgaaggc gaatacaaac
    13561 cagaagcaag ggcatggcaa acggccaata atacctatgg tttttccaac ggtcttggcg
    13621 tcgtggaggt gattattgct ctgctggttt tggctaatcc tgtcaatcgc tggttaggtt
    13681 tattgggagg actgatggca tttaccacgc cgctggtaac actctcattt ttaatcacca
    13741 ccccggaggc atgggtaccc gcattgggtg acgctcatca tggtttccct tatttatccg
    13801 gtgctggtcg cctggtattg aaagatactc tgatgctggc aggtgctgtc atgataatgg
    13861 cagattcggc gcgggaaatt cttaaacaac gcagtaatga atccagttca acgttaaaaa
    13921 ctgaatattg ataatccgaa cactgtctgt tgttcacttt ttcaggcgtc aggccccctt
    13981 tctgatgatg aagcctgacg ccatattacc ggagatatga cagagtgaag atcaatatca
    14041 ctcccctgtt aaaagattct tgtcgtctca ggtaaaatat atgattaata tcaatcatgt
    14101 atttttacag caagacaacc acgaacaaat tcaggattaa aatgttttac cgactcccca
    14161 acataaccca aatcaagcga actaatttgt gccatttctt tctctgtcag tgagaaatcc
    14221 cagatagcaa aattttcttc gatacgctgt tgatgtgtcg atttcggaat aacgaccact
    14281 ccccgttgaa tattccaacg cagaatgact tgagaaatcg atttttgatg ggcatctgca
    14341 atactctgaa gcagattatt ttcaaagggt ttatgtcgtc caccacctaa cggagcccat
    14401 gcttcaggct gcacgttata atacttcatg gttgccagcg cctctggttg ggcaaaatag
    14461 ggatgcaact cgacctggtt gaccatcggt ttaactctta ccgtttcaca aaagttcgcc
    14521 agaacatgag gatagaaatt ggaaacccca attgctttta atttgccctc ttcataggca
    14581 tcttcgagtg cacgccacgc actgaaataa tcgcgcatag cctggtgcaa taaatagagg
    14641 tcgaagtact ctagtcccga tttttttaat gatgcttcaa tacctgctgc tgccgtatcc
    14701 tgattcaaca tatcctgcac ccacaattta gacgttataa ataactcatc tcgggtacat
    14761 aaaccttcag atatagcttc acgaacggct tcaccgacgg catcttcatt accgtatacc
    14821 gctgcagtgt cgatgagtcg ataacctgtg cggatagcgt tcaacactga ctgtttgcac
    14881 acgtttttat cggtaacctg aaaaacacca aatcccatca tcggcatttt cagattgtta
    14941 cttaatacgg aaaattccac tgtcagtccc tccattaggc atgaacaatg agtctacgtt
    15001 aaaacgtaac ctcaaattag tacgtggatt ttgatatcac ttatgcaaaa aattcattaa
    15061 taatgcagga ctgaaacctc tctattttcg gggacaacga agcagacgct accagtgctt
    15121 ttgccttcgc ccttgctgtt tttgatacac ttagggccca gggcataacg aaaatgtgcg
    15181 atatgacgaa gaatcaatgg agaatgtgat gatcaggcag aagattctac agcagctcct
    15241 ggagtggatt gagtgcaatc ttgagcaccc tatttcaatc gaagatatcg cacagaaatc
    15301 tggctacagc agacgcaaca tccagcttct gttccgaaat ttcatgcatg tgcctttggg
    15361 agaatacatt cgcaaacgaa ggctttgtcg tgccgccatt cttgtccggc tcaccgcgaa
    15421 atctatgctt gatattgcac tctctttgca ttttgattca cagcagtcat tcagccgcga
    15481 atttaaaaag ttattcggct gctctccccg tgtataccgc caccgtgatt attgggatct
    15541 cgcaaatatc ttcccttctt ttttaatacg tcaacagcaa aagaccgagt gtagattagt
    15601 caactttcct gagacaccta tttttggcaa ttcatttaaa tatgatattg aagtgtcgaa
    15661 taaatcaccg gatgaagaag tcaaactgcg acgtcatcat ttagccagat gtatgaataa
    15721 ttttaagacg gatatctatt tcgtctccac gtttgaaccg tcaacaaaat cggtcgattt
    15781 gctcacggtt gaaacttttg ccggtacggt atgcaaacaa accgattcag agatgccgaa
    15841 agagtggaca attaaccgag gactgtatgc ctctttccgc tatgaaggtg agtgggagca
    15901 ctaccctgaa tgggcgcgaa acctctatct gatggaatta cccgccaggg ggttagccag
    15961 agtgaacggc agtgatattg agcggtttta ttacaatgaa aatttcgtcg aaattgatag
    16021 caataacatt gtttgcgaaa tttttatccc cgttcgtccg gtttagtttt cgctatatct
    16081 aaatgaattt ataacgagac tactgtcagg aaattcaata aataatggcg ttgaagatgt
    16141 caacgccatt attgtttatt agaatgttac tttcatattc ttaaaattat ttcatcgcct
    16201 cctcgtcctt tgccgtatcg gcatccaccg cggtaatggt caccgtcact ggcttcattt
    16261 cgtacccgcc ataggtcagg gcgttaaatg tgaacgtata gacaccaggc ttgctggtac
    16321 taaatttacg cgtgattttt ccgctcacga aatggtcatt tttcgacggc aggaactgat
    16381 aatccttttc cgtcacccct tccggcgtct gaatgtcgac ccacatattc ccgccaacgg
    16441 ggttgtttgc cgacatcagt gtcacctcca gcttcgcagt cgatttccca tccgccacca
    16501 gagattgcat atcagatgat gcggtgagta ccgcgttttt atcatccgca acgaatttag
    16561 attcggcagt tttcgttgat acctgaccgt tggtctgact cacttgcgcc ttgagtgtgt
    16621 atgtcgccgc aacagtagtg gtagcggtga aaatagcctg cccttgctca ttcgttgtcg
    16681 cagtgccatt cggttccagg tttaacgaag ccgggctggc agtcagcgtc acttcgctat
    16741 ctttcaccaa attgttattg gcatcagtca ccgtaacttt atacgtttgt ttggctttgc
    16801 cgtccgccag cgcatagttg cccatcacct caataccgga gacggttgcg gtagccttat
    16861 cggctacaaa cgttatagtc tgcgtatccg aaggctggct attccccgcc gatgccgtga
    16921 tctcatacgt tccggcagtc actgaaacca cctgcaactc tgctttcccc tcaccgttgg
    16981 ctttcaccca gacattatcg cctgtaagcg gcttgacacc ccggggcaga ttaaaggtca
    17041 ccaggctccc tggaacaggg ttaccatttt tatcctcaac ggtcgccgtc agcgtaaagg
    17101 catctttgcc gtttgccact tttggcgcgg cgctatcaac ctccagactc gcctgaccgg
    17161 tactgaaatc cgccttgaat ttcaccgtaa ccgtcttctg agcattattc actgaggccg
    17221 tgatgctgtg ttccccggca accgttgaca tcagctcaat gtcggcttta cctgccgcat
    17281 cggttgttac cgtattcccc gtcttgctgg tcacaccttt cggcagagtc agcgtcacat
    17341 tttgtccctg caacgggtta ccataggtgt ccacaacggt cagggttacc tggttagtcg
    17401 attgtccatt agccagctgg ttatcaacct tcaccgtcat atcacgaatc tcagccttag
    17461 atgcgtcacc agcaacattc agcaccagtg gctgagcaac ggcattttgg ccgttcactc
    17521 gcggcatcac agacaactgg cccgcggcag atcccagagt caaggtcgcc acgtagaccc
    17581 cattacttgt ttctgtccag tctcctgttg aaggtcgctc tgtccccgtg ctggcggcac
    17641 cgctaaacac tggtgcatct ggcttcatcc cggtgaccgg gttcccgtac gcatccttca
    17701 cggtgaaggt gagttccgtc gtcgttttga cggtcggagc cttattgtcc gcaacaagcg
    17761 tagagttcgc tgatgacatc tctccggcga tgaccgtcaa ctgcgcggct tcggtggctg
    17821 ctggctggcc gttgaatagc ggcatgacac gaagctcgcc cgtctttccg cctgtagtta
    17881 acgtagcaac ataggaaccg tcaccttttt cggtccaact ggaaacggtc gccccttcag
    17941 aggcggtccc cgtcaaactt gccgacaacg aaagaccact gatagcgttg ccatgtgcat
    18001 cttttgcaat aagcgtcacg gttgtacttt cgccggcttt tacgtgatct tctgcgacag
    18061 agactttcga ctggtttgaa gataacgcat cagccaccac ggttactttt gccgcatttg
    18121 ctgccgcatc ctgtccattt agcttcggca taacttctaa ttcacccgcc gtagagccga
    18181 gagtaatctg cgcagtccac gtcccatcac cattatttgt ccagcccgat gccgtagaac
    18241 ctacagcagc ggcacccgct aatgacggcg cttccggcgt gaggctggtc acagggttgt
    18301 cataggcatc ttttaccgtc cagattgccg taactgtacc cccaaccacc ggtttatcag
    18361 gattcagggt gatggacgaa tgtgctgcat caagcggccc ggcaacaaac ttcagcgttt
    18421 gttgtaatgt gcccagctga tactgctcgc tgaaagcacg cacaacaacg tttccggcac
    18481 gggtcgaaga aaccgtagcg ctgtaaacgc ctggttttat ctccgaaatt gtaccaatgg
    18541 tgacaccatt agtgtcttgc ggaacaaatc gcaagcggct ggcctctccc gtcaccgggt
    18601 taccatcagt atccaccgca gtcagcgtca gcgtgtaggc ttgctgacca tcagcaacca
    18661 cgtcacctga cggctcgtta gcgctcaggg tggagtttgc cacatccatc atcgtggccc
    18721 gcagttctgc agtgacggtt ttgctcatgc catcaacact aacagtaatc gttgcctcac
    18781 ctgactgcgt tccggtagta aagacagact gatacacccc tgcttcagtt tcggtgaact
    18841 cacccagtgt tggctttgcc tgtgatttag tggccttcag ggtacgagtc acaatatttc
    18901 cagccggttt gaaagttagt tcagtcttga tctgatcttt catgcccgtg actggctggc
    18961 cctcggcgtc gcgcagagac agcaccagcg gtctttgctc attaccgtta gcaagcattt
    19021 gaatacggct ctgaccgtca agcgttaacg ccgtgcgatc ggcactcata cctgctccgg
    19081 taatgaccac ctctgtctgc acgcgttttg aggcattgcc tttgttatcg taggcaaccg
    19141 cagaaatcgc ataataattg tctttgcctg gacgataagc cgggagcgtt acttgccact
    19201 gactaccctg accggtaatt ttgccacctt cagccagtaa tgacggcgct tcccactgca
    19261 catttttcag tccgtgagtt gctttgctga ccacaagccc cagggaaagt gtctgaccgc
    19321 ccttaccttc aatacgctca ggcagagcaa tacggatcac ttcagatttg tggtactcaa
    19381 gaacgatgtt gttattacgc tcaaccaggt catagcggct gcctgccagc attcgacgct
    19441 cgcgaatgct gtctgtatcg agttgtttct ccagaggttc gccaatccga taattaactt
    19501 ccaggccaaa gcgagtgtca ttctcaccgc tcttgccctg cttatgcccg gcgctcagtg
    19561 tcagaagagg cactggcgta taggtcacct cggcagaaat agcatgcggg tctttctggc
    19621 gcttatcttt accaaacagc ccgacttcat cgccataata ctgttcatac atcaggcttg
    19681 cgccaagctg cggccaggca ggtaaatagc cctctgcgcg gatatcccaa ccattcgccg
    19741 ggcgttcctg ataatcctca atatccggcg attttttcca gccagaagcc cgaatataac
    19801 cattggcgct cagtttcaga taatcgcgcc agtattccgc accaacacca atgcgggtat
    19861 gactacggga taaatcatga tcgataaaag tattcacccc cgccatccag tcatttcctg
    19921 aaaaatgacg ccagccaaaa ccaatatttg actgagtacg atcgtctgta cgatgtattg
    19981 ccccctgagt gaacaacata tttgtcggcg tatcataaat cggataaagc atttccagcg
    20041 aagaatcctt cagcgagaaa tctttatcga cattcagttt gacgcgcgct gtaccatatt
    20101 tcccgagcca ctcctgtatt tcctggttag ctttagcggt ggccattccg gtaataaaat
    20161 tacgtgtcgc atcgctatct ggctgactgc ttaaaaatgt cccggcattt gcggcaaacg
    20221 acgcgacatt tttctccaca ttattatcag cagttaccgt ggtattttcc atgctcaacc
    20281 gtggctgaac cgcatgctgc gcatgtgccg ccattactgg ggtaaaggtg acagcgagtg
    20341 gaaaaagaac ctgaacagag atatttgccc acgccacgca gcgggccaga actgaataac
    20401 gaaatagtgg ttgtttatga cctgttttat aacgtgacat tgagataatt tcctgatgaa
    20461 cgaattaacg aacataggca gacacaaata atggcgtctg cttgtcagaa aaaccggaga
    20521 tttcccggtt tccttttccg cagaaaagga aataccttta tgtaagaaag tacaccgcca
    20581 cctaatatat ccagtgcctg tagccactca tcaggtggtt gggcgcggga ttatattttt
    20641 atttatgggt aaaaatttag ctttttgtgc aagtcattaa atagatacct aagttatatt
    20701 tcttactctg cggacagtga ataattcatt attcagagtg aataataatg acctgtcttt
    20761 aatttaccac gattggcaat acatcgaagt aggtatatac gccaggataa accaatatat
    20821 tttttatgga tattcatttt cattcatcag actgtaaatt gcggagatat cgaacttgca
    20881 ctacccatcg caacactcaa acagatttat gaatttcgtt cataacccct gacaaatcct
    20941 gccgtctaat cgagcttttc cctttacgtt atgcttctca tgaacgataa cacaacttgt
    21001 tcatgaatta accattccgg ataaactatg ggatcaaatt caggcatcga aaggaactga
    21061 ctggtttccg ttaatgaatt aaatatctat tactctcccg agagtaaaac tatcaaacca
    21121 atgagtaacc gatacagcct cgcaaattct ttcagataat taacccgtca ctgcggcacg
    21181 ttctctcgta aggcgtgcct gcagtgtaat ccagtatgta tccggcatag ttgacggttt
    21241 aatagaactc aattcctctc ccaggtcacc aacaacgata tcttgtataa caccccagga
    21301 gatacgacat tatgatgaga tgcgatatgc tcctcagcgc caatcaacgc ccggactggg
    21361 ccagtcttga gatcagactc attcccggaa ctgacttcag gcgacatata tcaatgacgt
    21421 tggtgcaata aaagatagcc gatgtaatac atccattcaa atttgtgata catgtcaaaa
    21481 aattggttga ccaaactcgt tatttatata agggcactta cgaagtgcgc tcttttttaa
    21541 agcgaggaag taccaatgaa agagaataaa gtacaacaaa tcagtcataa actgattaat
    21601 atcgttgttt ttgtcgcaat tgtagaatac gcctatttat ttttccattt ctattaataa
    21661 cggcaataaa ctgttcactt cagtgatatt taaaatatgc atc
//
LOCUS       DAZGSP010000084        21677 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZGSP010000084 DAZGSP010000000
VERSION     DAZGSP010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.084"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..249
                     /locus_tag="ACHK74_003542"
     CDS             <1..249
                     /locus_tag="ACHK74_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFP0222292.1"
                     /translation="LVQVLVRTNDGGRQAVREYIVMTDELRNTLSGQSHATWGHYIDE
                     IIRQEKRRIRDQVLTMYQNDRIEKSEAALFIPAGEFPR"
     gene            246..536
                     /locus_tag="ACHK74_003543"
     CDS             246..536
                     /locus_tag="ACHK74_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP0222293.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFMLLGYSGWTVRTLCIRLYSLLQGKQASGRPWWYRHRTELPREWHGPE"
     gene            600..4616
                     /locus_tag="ACHK74_003544"
     CDS             600..4616
                     /locus_tag="ACHK74_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFP0222294.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAE
                     AGTDLSLLKRWLPRPQEMSMDAGDPVTEFRQVLENAGLVLEKDPEMDGEIHRVPTRDD
                     RRGSQSGAYKAYLDGRPSGWYRDYRSTDNSPTTWTFSRAENIDPLARLHLKASAQQNR
                     DEKERKLQQQYNKQAGYARSYLNGLPQATAHEYLTRKGIRAAPGVRLNNKNELVIPFS
                     NGRGEIRSYQRIPVTGGKDARILKGSEKSGNWFAFGTPENGRPLLFAEGYATAASLHE
                     ATGHPVLMTIDADNMINVAKNARKIWTDSPFVFCADNDHQRKINKGVSSAKKAAASTH
                     GEVIIPAFTEAEKAQGLTDFNDLDASRGRDAFQRAMNAQLEYIGIPTPNNDTTDRREA
                     IVIGNLIFTPIKDEKPQVSPENRQSTAPEPAVSEQNHPYDIPAEETDNQAYEDDYAAY
                     ASLQSVTAGTASPNEPQVNAVSAIDSNAKNTSAATAGDRNVLAQEPNADATVTTTAHD
                     TGTAETDSPSEPAEIQPVQQASPEPSAKDKPQENVVSATDNDAENTSSATTTLRSNDT
                     PETSVSDATGTQHTATTQPEEPQQVSMGSADDWSAFAEDLNADAPGNATVHSTDNAET
                     KTLSEPAGMQPVQQATPEPTEENKPQEEGVMSATDTGEESAAAAIDDDLNTVAEDLNA
                     DVTVMTTAHHTDTAETASPSEPAEIQPVQQATPEPTAEDKPKEERPVSATETSTENEA
                     ARPETPSLFSRLRRFLTGGKSRTAEPPVIQENTLTEAETATEAAAPPSPPETSPEDTS
                     SAEPDTIIFAPRRPDSPQRHNLDEILKNLTHKEFPDRTALYSVDGEPAFLDRLYCLEM
                     VDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIVEYDIKVRMKLPAQRA
                     ALEKLRKEKEMAHDAIVTHRPTPELNRQSPEETTMAQPAPPVSPKAEAEQTAGSTAET
                     PHPEAPVTAKAEAPRADRPEQPAAPSSAPEKAPVSAQPQQPDTPEEEPPGKLRPGESV
                     TAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGNGLEELVKNHQKGDPVTLTLQAR
                     ETFRLSGETRDRIRNIWAITPVSNGITVNNDQPHAGQHILEFPMKTFSQVMSQMQQHW
                     PQIMQEVRSPKNPPNNLYIGEDRQPASAPQDQSMRLPLAGNAPNELIPAIASADKDNR
                     QLNLLLVHSADDHLQGVVRLNGTLYPALATPSADNRQLVINALTDNGLQFAGYGEAVN
                     HDENTHQRPSPQIMQFHLKQQDSPLFAAIHKPEEQPDKLFRSLGFEQTWKEWSDSQKA
                     EDRQEKTLQQAQSHSPGL"
     gene            4633..4983
                     /locus_tag="ACHK74_003545"
     CDS             4633..4983
                     /locus_tag="ACHK74_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP0222295.1"
                     /translation="MNMKKTLLVILTSISLASPAAVAATCRAGAAAEAGSKAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDVSITLPQFPSLDKILRDLENQVCDAVVDRVNE
                     HLPGNIDPWKDYNL"
     gene            4995..5690
                     /locus_tag="ACHK74_003546"
     CDS             4995..5690
                     /locus_tag="ACHK74_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFP0222296.1"
                     /translation="MSEAHPTSPVSPETDNSGDIYKQTLSTIAQSEQRAALVPGLVRA
                     LLCSSAALVISLTGNIIQYWHNSNVTREYFATDNGRLVRLAPTSQPAWSQSDVMTFGS
                     MTLSEAFNLDFVHYRKQITTLAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTATIG
                     AGTLVNQGQMENGIWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAASGT"
     gene            5701..6675
                     /locus_tag="ACHK74_003547"
     CDS             5701..6675
                     /locus_tag="ACHK74_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HFP0222297.1"
                     /translation="MQIHHLLGALLIVCGTVTAADNPGWQNARTPQAGGKTGQAPGNA
                     PAGGPPPATQLTGPLPPPASPLVQEAASLDSPLNAGEIRELRGRMADNERAISAPVTS
                     VVPRISSLTVNLSPGASLPLVRTALHNVSVVTFTDINGSPWPQSDPPYNAAPGHFDVQ
                     FNGNMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTNTLSQEIDSRLDLRIPR
                     QGPASPVVAVPSEKIALHDATLQAFLDGVPPDDPTVRRLKFSGNVPDTTIWQHGDDLL
                     VRSRAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSIHVTPELE"
     gene            6679..8010
                     /gene="traO"
                     /locus_tag="ACHK74_003548"
     CDS             6679..8010
                     /gene="traO"
                     /locus_tag="ACHK74_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFP0222298.1"
                     /translation="MSAEQDTAAKSGKKLSALLVLGGVIIIGGGYLLLTWLSPENSAS
                     LSSVNINSTTTGSGRTVVESQHYRDLLRADNERGAAEALRNNQTFIASLPRGLDTDHG
                     KNQRRDTQPADQAEGSGQPSTPPQADQTAREKQREALQKLLARINTQHPDARPPVVAA
                     AMWGTTGAPGISSHASPPGTGTSPQPRLQEASLATPAARTGIQLIPALTRVPAFIDTE
                     VDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGKGMDIHFDRMMWRGMFLKVNAYALN
                     EHTGMSSVASDVNHRWFKHIVLPSVLGGVGSVGSLYKDANTQVIQGNYGSITGRVGMP
                     DGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDISPPS
                     SGTAPSVPPPSGRPLTQEEAERQASDRIQAAAIRKQNEMRQRYGIQQESHP"
     gene            8007..8720
                     /gene="traP"
                     /locus_tag="ACHK74_003549"
     CDS             8007..8720
                     /gene="traP"
                     /locus_tag="ACHK74_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFP0222299.1"
                     /translation="MKNEHEDPGSFGETTTPASGKSFLKRQYMGYSLPVWAGCVLIIL
                     VAVWYKFLRTPSEPPADAGFAVTPPAAESWAEPMPMKETGSGNTMSAPPVTPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKESITTLGDTIRKDEAWAQETRQLLDETRKQVHAL
                     QMQGPLQIPTGNNRQPAKTPPSRLQGMKIMSVEKGMAWIRWQGSAWAVREGDRLGNIT
                     ITRIEPSSRSVITSAGTLR"
     gene            8717..9247
                     /gene="traQ"
                     /locus_tag="ACHK74_003550"
     CDS             8717..9247
                     /gene="traQ"
                     /locus_tag="ACHK74_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFP0222300.1"
                     /translation="MMSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIVICGMLVALPSFINAGGTQMGFRADSFGPIAYVQPTTFGAA
                     AGAANAMLSLVKLAGVGFAMNGISIWRKSLLDGHTALSSSESISKGNVKFVVGVLLVF
                     IDRVIDASLASLGLAF"
     gene            9296..9694
                     /locus_tag="ACHK74_003551"
     CDS             9296..9694
                     /locus_tag="ACHK74_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HFP0222301.1"
                     /translation="MSRFRAMTARLCAAFIYVQLCLVACRDRLIRLLLLLPVILLPHT
                     AMANTLFDMIKTVGDGAKGSSSSFVAIGQVIGLVLFIGGLLGLKQIGKKPGAGLACLL
                     SILVGLCLAVVPELMGSGQKTLGLSPVSIS"
     gene            9751..10002
                     /locus_tag="ACHK74_003552"
     CDS             9751..10002
                     /locus_tag="ACHK74_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HFP0222302.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKTYEQNTATHRPGPGTLAFPAPATGHGIQR"
     gene            9917..10735
                     /locus_tag="ACHK74_003553"
     CDS             9917..10735
                     /locus_tag="ACHK74_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HFP0222303.1"
                     /translation="MNKTPPLIAPVRERWLFPLPQPDTVFSGESLLPPPVLSTPGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTVVLLEKNLTRVMRLKKHPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESELLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALKEIFSDERKRIRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRQMLREKMDGMALILLPDSFPDKKYVM"
     gene            10794..11153
                     /locus_tag="ACHK74_003554"
     CDS             10794..11153
                     /locus_tag="ACHK74_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222304.1"
                     /translation="MDTHSAGYQFTADSFRRFIIRTLTDDIPPDGDEKEIARFILRHP
                     AFNGGWLLFLAAEIHERSRTHNSITVTVSVDNYLSWMDIAVSGLPELPDMPFCDFFIR
                     RQQFIYPGVPPTKSGEE"
     gene            11153..11533
                     /locus_tag="ACHK74_003555"
     CDS             11153..11533
                     /locus_tag="ACHK74_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222305.1"
                     /translation="MKIHRISPETLITLILAHLAGKADSTAKEEHRLLRRFLRNDDGR
                     LAGILLNIAGILQFNRELSARHNYPATPLTEFSLRKRGKQLHLCLCSLRFFYIPPVFI
                     QNKRRKSIVVHLNKITYKQTHSIR"
     gene            complement(11506..12051)
                     /locus_tag="ACHK74_003556"
     CDS             complement(11506..12051)
                     /locus_tag="ACHK74_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222306.1"
                     /translation="MKAENKILNLFHATTMSRGLQIIKDGKIRTDAPSVICWSLETTP
                     GWIYLSDQLDNAVHWGNKIACLSKDELFFYIFRVQVQEENCYPDTDDLQYVSWLSPEE
                     AENTDLYTCLSLCHSCRTDKELYTGCEISDYAVLPATTLYTDNSGHPQYDIVNTLKKH
                     IGKDEYNQYESLRQRIEWVCL"
     gene            12276..15320
                     /locus_tag="ACHK74_003557"
     CDS             12276..15320
                     /locus_tag="ACHK74_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP0222307.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDLTAPGTLESLITALAERMNTAY
                     KNTGHKISFVFERDPERGRNEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRIRKLAEHAPPARFAQDPWRWTLSALKIRHDAFLD
                     TLEQALTHDSDGLLVRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGED
                     TSVFHAPSLNLQLFTTQPETHGSLIKAGDLWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPGGMKALGVKKTLLTYSSFISPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHGSGPVPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PEHTRNLFDVMYGAKKPATPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISNLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHMAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSAELLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQDYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQELGSLRARQI
                     LAENFPRGSATSLIEHRARTHDSENVIHDLAAELIRKQGYHL"
     gene            15320..15940
                     /locus_tag="ACHK74_003558"
     CDS             15320..15940
                     /locus_tag="ACHK74_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFP0222308.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGVRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     SPLTALMSDNTWLWPDSPGTPALTAGDWPVMETDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESHSHRVPDILYPLKTEKKECKDNQQPHCLHSW"
     gene            15898..17103
                     /gene="traW"
                     /locus_tag="ACHK74_003559"
     CDS             15898..17103
                     /gene="traW"
                     /locus_tag="ACHK74_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFP0222309.1"
                     /translation="MQRQPATTLLALLVGCAVIPSTHAYSVTVVASQPVTTQIMPELA
                     AIQGVMAEMVRMQTATGTAINQSSEKLASVIAQDGQATRQQMIFSNETRRLEEARKSF
                     SVPDSICSESASGVAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPTREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGELPGGDSQVRSLYQGAGTADSPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            17100..17669
                     /gene="traX"
                     /locus_tag="ACHK74_003560"
     CDS             17100..17669
                     /gene="traX"
                     /locus_tag="ACHK74_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HFP0222310.1"
                     /translation="MKDDNSTDKKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     GQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVNELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPAITLIRILACLLLMSAIAALCASKTLIVSYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            17742..19907
                     /locus_tag="ACHK74_003561"
     CDS             17742..19907
                     /locus_tag="ACHK74_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFP0222311.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIAIMGIVWFLFIGIRHVVRTGHQGNVFNN
                     GRDIVGMLSIVSGFMMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMALCKHAMNAGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLTVKGNGSNNQSTLDYFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPTDNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLAELADRAPTVSGVSAQGEAGDTEFLKEVKGAYQ
                     AQLQNSPYTPALGTATSASEYQASNTQNPQSALLKLLGNDLTLFTNQIATIHSGTGTT
                     SDQLNPLIKMKNIGDYTMVTTETIWAGYTTVRVLSSAGSNSIFGRLVNAVSGLSDAAK
                     AFLEASAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGVGNWIVSVLIGCAAGPLWA
                     ATHLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIMAGGTILNALFAAALM
                     NVQINSVTGVFSMAGFLMIYARVCTTLVAAVFALQAYLPDHVINFLGGRDGVNMLGNM
                     VSSVKDIIAGGNTNMRRTPGVREEKLKNINGDDKDGIKQ"
     gene            19995..20645
                     /gene="excA"
                     /locus_tag="ACHK74_003562"
     CDS             19995..20645
                     /gene="excA"
                     /locus_tag="ACHK74_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFP0222312.1"
                     /translation="MNKTKSRYPSWWAYYQLARNAYFLLGIPCLALYATAYTSYLLDD
                     KLTHSTSLFILAYGCYFLLFIPPLWLYLRTRAKKKKVQQVVQQIKESSPFAPTSDYEQ
                     LSFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVSL
                     PMIPLEISGISARTLANTIHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            complement(20710..20919)
                     /locus_tag="ACHK74_003563"
     CDS             complement(20710..20919)
                     /locus_tag="ACHK74_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFP0222313.1"
                     /translation="MSMSPAIANTFLLEMMKDKSKDVTLAVIYALGEGRCQADNIKRE
                     LRRLSESDDMEIKVAAIKALGRLYR"
     gene            21014..21145
                     /locus_tag="ACHK74_003564"
                     /pseudo
     CDS             21014..21145
                     /locus_tag="ACHK74_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutE"
     gene            complement(21460..21612)
                     /locus_tag="ACHK74_003565"
     CDS             complement(21460..21612)
                     /locus_tag="ACHK74_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0222314.1"
                     /translation="MPQRTFLTMLIVVCVTILCFVWMVRDSLCGFRVEQGNTVLVATL
                     AYEVKR"
BASE COUNT         5420 a         6257 c         5499 g         4501 t
ORIGIN      
        1 ctggtgcagg tactggtcag aacaaatgac ggcggcaggc aggctgtccg ggaatacatc
       61 gtcatgactg acgaactgcg caatacgctc tccggacaat cccatgcaac atggggacat
      121 tacattgacg aaatcattcg tcaggaaaaa cggcggataa gggatcaggt tctgaccatg
      181 tatcagaacg accgcattga gaaatcagaa gcagcgctct tcatcccggc cggagagttc
      241 ccccgatgaa acgcgaaggc ttctggcggg atgtaacacc accgctcaac atttatggtg
      301 tccccctgcc gtttaccatg atttacctgc tgttgtttcc gtatccgtca aaaacgacat
      361 tctgggtgtg cagcggcatt ctgctgtttt ttatgctact gggatattcc ggctggacag
      421 tccgcaccct ctgtatacgt ctgtacagcc tgcttcaggg taagcaggca tcgggacgtc
      481 cctggtggta ccgacatcgc acagaattac cccgtgaatg gcacggacca gaatagccct
      541 cttttgtcca tcatcccggc gtatcacaac gccctcatat ttaatccccg gagaacgaca
      601 tgaagtccag atatcgtacc tggcttgctg tcccgcctga ggagacagaa gccgtaaaaa
      661 atgcagtccc cccgttaaat ggccgcaaag cggtagcatg ggatccagag aaaaaactct
      721 ggtatgccga ggcgggtacc gacctttcac tgctgaagcg ctggttaccc cgtccacagg
      781 aaatgtcgat ggatgccggc gatccggtca cagaatttcg tcaggttctg gaaaacgccg
      841 ggctggttct tgagaaagat ccagaaatgg acggagaaat acacagagtg cccacacggg
      901 atgacagaag aggctcccag agtggcgcat acaaggccta tcttgatggc cgcccctccg
      961 gctggtaccg cgattaccgg agcactgata acagtcccac aacctggaca ttctcccgcg
     1021 cagaaaatat cgatcctctc gccagactgc atctgaaggc atccgctcag caaaatcgcg
     1081 atgagaaaga acgtaaacta cagcaacagt acaacaaaca ggccggctat gcccgctcct
     1141 atcttaacgg gttgccccag gcaacagcac atgaatacct gacacgcaag ggcatccggg
     1201 ccgcgcctgg cgtgcggctg aacaataaaa atgagctggt tattcccttc agcaacggcc
     1261 ggggggaaat acgttcgtac cagcgtattc cggtcaccgg cgggaaggat gcccgcattc
     1321 tgaaaggctc tgagaaaagc ggcaactggt tcgccttcgg aaccccggaa aatggccgtc
     1381 ccctgttatt tgcggaaggt tacgctactg ccgcctccct gcacgaagcc accggccatc
     1441 cggttctgat gacaattgat gccgacaata tgattaatgt ggcaaaaaat gcccgcaaaa
     1501 tctggactga cagccccttt gtcttctgtg ccgacaacga ccatcaacgg aaaattaata
     1561 aaggtgtttc aagtgcaaaa aaagctgccg caagcacgca tggcgaagtc attattccgg
     1621 cctttacgga ggcagaaaaa gcacagggac tgaccgattt caatgacctg gacgccagcc
     1681 ggggacgaga cgcctttcag cgagccatga acgcacagtt agaatatatc ggtatcccca
     1741 cgcccaacaa tgacactact gaccgccgtg aagctatcgt gatcggcaac ctgatattca
     1801 caccgataaa agatgagaag ccacaagtgt cccctgaaaa cagacagagc acagcccctg
     1861 aaccggcagt gtcggaacag aatcatcctt atgacatccc tgcagaagag acagacaatc
     1921 aggcatatga agatgactat gcagcatatg catccctgca gtcggtaact gcagggacag
     1981 cctccccgaa tgaaccgcag gtgaatgctg tttctgccat tgacagcaac gcaaagaata
     2041 cctctgccgc caccgctggc gaccgaaatg tattagcaca agaacccaat gctgacgcca
     2101 cggttacgac cacggctcac gacaccggca cagcagaaac agattcacct tctgaacctg
     2161 cagagattca gccagtgcag caggccagtc ctgaaccatc ggctaaagat aaaccacagg
     2221 agaacgtagt gtctgctact gataacgacg cagagaacac ctcttctgcc accactaccc
     2281 tccggagtaa cgacacaccg gaaacatccg tctccgacgc aaccgggaca cagcacaccg
     2341 ccacaacgca acctgaagaa ccacagcagg tctccatggg cagcgctgac gactggagcg
     2401 catttgcgga agacctcaat gctgatgctc cggggaatgc cactgttcac agcaccgaca
     2461 acgcggagac aaagacactt tctgaacctg ccgggatgca gcctgttcaa caggcaaccc
     2521 ctgaaccaac ggaagaaaat aaacctcagg aagagggcgt tatgtctgct actgatacag
     2581 gtgaagagag tgccgctgcc gctatcgatg acgacctgaa cacagttgcg gaagacctca
     2641 atgctgatgt tactgtgatg accacagctc accacaccga tacagcagaa acagcctcac
     2701 cttctgaacc tgcagaaatt cagcccgtac agcaggcaac ccctgaacca acggcagaag
     2761 ataaaccgaa ggaagagcgt cctgtatctg ccactgagac cagcacagag aatgaagcag
     2821 cccgccctga aacaccatcg cttttcagcc gtctgcgccg ttttctgacc ggtggaaaat
     2881 cacgtaccgc cgaacctccc gttattcagg agaacacgct tacggaggcc gaaactgcga
     2941 cagaggccgc agcaccaccg tcacctcctg aaaccagccc ggaagacaca tcgtcagcag
     3001 aaccggacac gattatcttt gcgccccgcc gtcccgacag tccgcagcgg cacaacctgg
     3061 acgagatcct taaaaatctt acacacaaag agtttcccga caggactgcg ctctacagcg
     3121 ttgacggtga acccgccttc ctggacagac tctactgtct cgaaatggtg gatggcgcca
     3181 gcgcggatga caaaaaggtc ctggctgccc ttgctgtggc caccaacttc tatggcggcg
     3241 ttatcgagct gaccggcagt gatgccttta aacaaaaagc catgcagctg attgtcgaat
     3301 atgacatcaa ggttcgcatg aaactcccgg cccagcgggc agcactggaa aaactccgca
     3361 aggaaaaaga gatggcgcat gatgccattg tcacccacag acccacgccg gaactcaacc
     3421 gccagtcacc ggaagagaca acaatggccc aaccggctcc ccctgtgtca ccgaaggcag
     3481 aggctgaaca aacagcaggc agtacggctg aaacaccgca tccggaagcg ccggtcacgg
     3541 caaaagctga agccccccgg gccgacagac cggaacaacc cgccgccccg tcgtctgcgc
     3601 cagaaaaagc ccctgtcagt gcacagccgc agcaacccga cacaccggag gaagagccgc
     3661 ccggcaagct cagacccggt gaaagtgtga ctgccgtcct gacaaactac ggagaaaaag
     3721 agtatgcgcc cggaaaaggg cgctgctttt ttgtggagct caccaaccgc agtggcaaac
     3781 gggaatactg gggaaacggc ctggaagaac tggtcaaaaa ccatcagaag ggtgaccccg
     3841 tcacactgac actgcaggcg agagaaacat tccggctgtc cggtgaaacg cgggatcgta
     3901 tccgtaatat ctgggccata accccggtca gtaacggcat taccgttaat aatgaccaac
     3961 ctcatgcggg tcagcatatc ctggaatttc cgatgaaaac attcagtcag gtaatgtcac
     4021 aaatgcaaca acactggcca cagataatgc aggaagttcg ttcccccaaa aatcctccca
     4081 ataatctgta cattggtgaa gacaggcaac ctgcatcagc acctcaggat caatcaatgc
     4141 gactccccct cgctggtaat gcccccaatg aactgatacc agccatcgcc tctgccgata
     4201 aggacaatcg tcagttgaat cttctgctgg tccacagcgc agatgatcat ctgcagggcg
     4261 tggtccgcct gaacggcacc ctttacccgg cactggccac cccgtcagct gacaaccgtc
     4321 agctggtcat caatgccctg acggataacg gtctgcagtt tgccggttac ggtgaagctg
     4381 ttaaccatga tgagaatact catcaacgtc cgtccccaca aataatgcag tttcacctga
     4441 agcaacagga ctccccgttg tttgccgcca tccacaaacc ggaagagcaa ccggacaagc
     4501 ttttccgctc tctgggtttt gagcagacct ggaaagagtg gtctgacagc caaaaggcgg
     4561 aggacaggca ggaaaaaact ctgcaacagg cacaatccca cagccccggc ctgtgagccg
     4621 gtaaggacga ccatgaacat gaaaaaaacg ctgctggtca tactgaccag catctcgctg
     4681 gcttctccgg ctgccgtagc agcaacctgt cgggcaggtg ctgccgcaga agccggcagc
     4741 aaagccgcct gggagcgggc gcgcacagcc agccaggcat ggtcacagcg agaaaacgac
     4801 gtcgcctcct ccctgcagtc ctgcctgtcc cgcattaagg acgtcagcat cactcttccg
     4861 caattcccca gcctggacaa aatccttcgc gatctggaaa accaggtctg tgatgccgtc
     4921 gtggatcggg tgaatgaaca cctgccgggg aatatcgacc cctggaaaga ctataacctc
     4981 tgaacaggag ccccatgagc gaagcacacc ccacatcccc tgtctctccg gaaacagata
     5041 acagcggaga tatttataaa caaacgctca gtaccattgc acagtcagaa caacgggccg
     5101 cactggttcc cggactggtc agggccttgc tgtgtagcag cgcagctctc gtcatcagtc
     5161 tgaccggcaa tatcatacag tactggcata acagcaacgt aacgcgtgaa tactttgcca
     5221 ccgacaatgg tcggctggtc cgcctggccc ccaccagtca gccagcctgg agtcagagcg
     5281 acgtcatgac gtttggcagc atgacgctga gcgaagcttt taatctggat tttgtccact
     5341 acaggaaaca aatcacgaca ctggcccccc gcttttccga agccggtttt gccggctatg
     5401 tgaatgctct tcaggcctcc aacattctgg acaccattaa aaaggagaaa tttaacctca
     5461 cagccaccat cggggccggc acgctggtga atcagggcca gatggaaaac ggcatctggt
     5521 tctggaaatt ccagtatccg gtcaggatgc gtctggtcgg tcagaccagc acccgccccg
     5581 agcaggcctt cacctttgaa atcactgtcc ggcgcgttga cccacgaatc aaacccggcg
     5641 gcatggaaat cagccagatg gtttcccgta acgccgcatc aggaacctga gactcatatc
     5701 atgcaaatac atcatcttct tggcgcactg cttattgtgt gcggcactgt cacggctgca
     5761 gataacccgg gctggcagaa cgcacgaacc ccacaggctg gcggaaagac aggacaggct
     5821 cccgggaacg cccccgcagg cggccccccg cctgccacac aactcacagg ccccctgccg
     5881 ccacctgcct ctccccttgt tcaggaagcc gcatcactgg attcccccct gaatgcaggt
     5941 gaaattcggg aactgcgggg acgaatggcc gataacgaac gggccatcag cgcacctgtc
     6001 accagtgtgg tgccccgtat cagctccctc acggtaaatc tgtcacccgg ggccagtctg
     6061 ccgcttgtcc gaaccgcact gcataacgtc agcgtggtaa cgttcacgga catcaacggc
     6121 tccccgtggc ctcagtcgga tccgccttat aacgcggcac ccggtcactt tgatgtgcag
     6181 ttcaacggca atatggtctc cgtcactccc ctgcgcgcct gggctgcagg gaatgtaacg
     6241 gtctacctca aaggactgtc tgtaccggtc attctgaata tcaccagtgg tgaaaccgac
     6301 accaataccc tgagtcagga gatcgacagc cgtctggatc tgcgcattcc ccgtcaggga
     6361 cctgccagtc ctgtggtagc cgtaccatca gagaagattg ccctgcatga tgccactctg
     6421 caggcctttc tggacggtgt tcctcccgat gatcccaccg tgcgtcggct gaaattctcc
     6481 ggaaatgttc cggacaccac catctggcag cacggcgatg atctgctcgt gcgatcgcgg
     6541 gccatgctgc gggatgaatt tgaacagacg ctggcatctg ctgacggtac gcgtctgtgg
     6601 aaactgccgg tgaccccctt actcaccttc tccgtgaatg gtcagtccat tcacgtcacc
     6661 cctgaactgg agtaaatcat gagcgcagaa caggacacag ccgcaaagtc cggcaaaaaa
     6721 ctgtcagccc tgctggttct cgggggcgtg attatcatcg ggggcggata cctgctgctc
     6781 acctggcttt cccccgaaaa cagcgcatcg ctctcgtcag tcaatatcaa cagcacgaca
     6841 acaggcagcg ggcggaccgt ggtggagtca cagcactacc gggatttact gcgtgctgac
     6901 aatgagcgcg gggccgcaga agcattacgt aataaccaga cgttcatcgc cagtctgccc
     6961 aggggactgg atactgatca cggaaagaat caaagaaggg atactcagcc tgctgaccag
     7021 gccgaggggt ctggtcaacc atcaacgccc cctcaggcag atcaaacagc ccgggaaaaa
     7081 cagcgggaag cgttacaaaa actgcttgcc cgcatcaaca cacagcatcc tgacgcccgc
     7141 cccccggtag tggcggctgc aatgtggggc accactggtg ctcccgggat aagctcacat
     7201 gcctcaccac ccggtaccgg cacatcacca caaccacgtc tgcaggaggc cagtctggca
     7261 accccggcag ctcgtacagg gatacagctg ataccggcac tgacccgcgt acctgccttc
     7321 attgatacgg aagtggactc tgacaatgct gtctcccgcg tggtggcctc cgtacctgcc
     7381 ggcccatggg ccggggccgt cctctacagc caggacgcca gactcgccgg caagggaatg
     7441 gatatccact ttgaccggat gatgtggcgg ggcatgtttc tgaaagtcaa tgcatatgcc
     7501 ctgaatgaac ataccggcat gtcatccgtg gccagcgacg tgaaccaccg ctggtttaag
     7561 catatcgttc tgccgtctgt gctcggtggg gtgggcagtg tcgggagtct ttacaaagac
     7621 gccaacacac aggtcattca gggaaactac ggcagcatta ccggcagggt gggaatgcct
     7681 gatggcgagg cgctggctgg cgtcattgca ggcggcatgg ccgaacgggg cagtcagttg
     7741 ctgacccgcc aggcggagag cgaaccttac cggcaggtga cagtgtcacg cggagaggtt
     7801 gtctccattc tgtttgttga gccggtcatg agcaacgata tcagtccccc ctcctccggc
     7861 actgcccctt ctgtgccacc gccctccgga cgcccgctca cccaggaaga agcagagcgt
     7921 caggcgtctg acaggatcca ggcagctgcc atacgtaaac agaacgaaat gcggcaacgt
     7981 tacggaatac aacaggaaag tcatccatga aaaacgaaca tgaagatccc ggcagttttg
     8041 gcgaaacaac gacaccggca tccggcaaat ccttcctgaa acgccagtat atgggataca
     8101 gcctgccagt ctgggccggc tgtgtcctca tcatcctggt cgcagtctgg tacaagtttc
     8161 tgcggacacc ctcagagccc cctgcagatg caggcttcgc tgtcacaccg cccgcagccg
     8221 aaagctgggc agagcccatg cccatgaagg agaccgggag cggaaacacc atgtccgcgc
     8281 ctcctgtcac cccacaggca gacatgcagc agcttgcccg tgacctgaaa gcagagctgg
     8341 acaggcggga tgaggaaatc cgacgtaccc tgaccatgct gaaagagagc atcacaaccc
     8401 ttggcgacac catacggaaa gatgaggcct gggcacagga aacccgtcag ttgcttgatg
     8461 aaacccgtaa gcaggtgcat gccctgcaga tgcagggacc actgcaaatc ccgaccggga
     8521 ataacagaca gcctgcgaaa accccgcctt cccgcctgca gggcatgaaa atcatgtcgg
     8581 tggaaaaagg aatggcctgg atccgctggc agggtagcgc ctgggcagta cgggaaggtg
     8641 acaggctggg gaatatcacc atcacccgga ttgaaccgtc cagccgctca gtcatcacct
     8701 ctgccggtac attacgatga tgagcattga tgccctgaca gccatagaga actttgccag
     8761 cggtatattc agtgcaggca tggagtttct cttcacctgg ggagagctgc tggggattat
     8821 cggcctgatt ggtcatctga tgcgcgcccg ggcagaaggg cgtcacagta tgggaccggg
     8881 aaaatttatc gccggtatcg tcatctgcgg catgctggtc gccctgccat ccttcatcaa
     8941 tgccgggggt acccagatgg gattcagggc tgattccttc ggccctatcg cttatgtaca
     9001 accaaccacc tttggtgccg ctgccggtgc cgccaatgcc atgctctcac tggtaaaact
     9061 ggccggagtg gggtttgcca tgaacggcat ttccatctgg cgtaaatccc tgctggacgg
     9121 gcataccgcc ctgagcagca gtgagtccat cagcaaagga aacgtcaagt ttgttgtcgg
     9181 cgttctgctg gtttttatcg atcgcgtcat cgatgcctct ctggcgagtc ttggtctcgc
     9241 cttctgatcc acattcattt tcagtaaatc agtctgttag ttaaggagac actatatgtc
     9301 acgattccgt gccatgacag cacgtctgtg cgctgcattt atttatgttc agttatgcct
     9361 ggtggcctgc cgtgaccggc tgatacgcct gttacttctg cttcccgtga tactgctgcc
     9421 ccacacagcg atggcaaaca ccctcttcga tatgatcaaa accgttggtg atggtgcaaa
     9481 aggctccagc agttcatttg tggctatcgg tcaggttatt ggcctggtgc tctttatcgg
     9541 cggcctgctt ggcctcaaac aaatcggtaa aaaacccgga gcagggctgg cctgcctgct
     9601 gtcaattctg gtcggcctct gccttgccgt cgtaccggaa ctgatgggca gcggacagaa
     9661 aacgctcggt ctttctccgg tttcaatcag ttaatccgca aaggcaccgg gaaaccggtg
     9721 ccgtcttcac acaatgaaga ggaccagacc atgtcctgtg agctgccccc tttcaccctg
     9781 ctcagaacag agcacggcaa cgtgattgcc cgcgacaacc gggataatca ggagtttgtg
     9841 tttgcagagt gttattccac cgccatcgca gcactgattc tggctgccgt caccctgtat
     9901 acagagagaa aaacgtatga acaaaacacc gccactcatc gccccggtcc gggaacgctg
     9961 gcttttcccg ctcccgcaac cggacacggt attcagcggt gaatccctgc tgccaccacc
    10021 cgtgctctca acacccggac gctgtccctg ctgtcgcagg accgtcactc accgcttcat
    10081 tctggaggat tcatggccat tacagcagat ggctgacacc tgccgggata cggtcgtcct
    10141 gctggaaaaa aacctcaccc gggtgatgcg cctgaaaaaa catccggtac cggaaaacgc
    10201 tgacgaaaaa aagaaacaca cccgaaccct gcaggatgcg gagcggtccc tggcacaggc
    10261 ccgcctgtcg gccaggcgac tggccctgcg tcatgtggaa aagtcacaaa tcgtcacaac
    10321 agatgcactc agcgaaaatg aaagtgaact gctgcagcct gaaggcccgc cctttcatct
    10381 ctgtgccttc tgccacgcct ggcactgcct gaacggctac gctgccgcac agggggtgat
    10441 ggtctggctc cctgatttgc atccggcctc ggtagttgct ctcaatgcac gggcactgaa
    10501 ggaaattttc agcgatgagc gtaagcgaat cagacaggga cgggccgtac tgaatgccct
    10561 ggtacagaac cgcctggccg ttgaggagaa atttcgcacc tggcgtccgg cggattttgc
    10621 tgatgcactg cgccgctggc cgccggcaca gcgtcaaatg ttgcgggaga aaatggacgg
    10681 aatggcgctt atcctgctgc cggattcgtt tcctgacaaa aagtacgtca tgtgacacac
    10741 accggtaccg atgacattct gccggcacac attcaactta cagaggaaca aaaatggata
    10801 cacactctgc cggatatcag ttcactgcag attcattccg gcgttttatt atacggacgc
    10861 tgactgacga tattccgcct gacggagatg aaaaagagat tgcccggttc attctccgcc
    10921 acccggcatt taacggcggc tggctgctct ttctggctgc agaaattcat gaaagatccc
    10981 gtacccataa cagcatcacc gtcacagtga gcgtcgataa ctatctctca tggatggata
    11041 ttgccgtgtc cggtctcccg gaattaccgg atatgccctt ctgtgatttt ttcattcgcc
    11101 gccagcagtt tatttatccg ggtgtacccc cgacaaaatc aggagaagaa tgatgaaaat
    11161 tcaccgcata tccccggaga cgcttataac actcatcctc gcacacctgg ccggaaaagc
    11221 tgacagtacc gccaaagaag agcaccggct actccgtcgg ttccttcgga atgatgacgg
    11281 caggctggcc ggcatactgc tgaatattgc cggaatactt cagttcaaca gggaattaag
    11341 cgcccggcat aattatccgg ccacacccct gactgaattt tcattaagga aacggggaaa
    11401 acaacttcat ctctgtctct gcagtctccg tttcttttat attcctccgg tatttataca
    11461 aaacaaaaga agaaaatcta ttgttgtaca tcttaataag atcacttata aacaaaccca
    11521 ttcaatacgc tgacgaagtg attcatactg attatactca tctttgccta tatgtttttt
    11581 aagcgtatta acaatatcat attgaggatg tcctgaattg tcagtataca gtgttgttgc
    11641 aggaaggact gcataatctg atatttcaca acctgtatac agttctttgt cagttcggca
    11701 ggaatggcaa agagaaagac atgtatacag gtctgtgttt tctgcctcct caggagataa
    11761 ccacgacaca tattgcaaat catctgtatc aggataacaa ttttcttcct gaacctgaac
    11821 tctgaaaata tagaaaaata attcatcttt tgaaaggcat gctattttat tcccccaatg
    11881 caccgcatta tcaagttgat ccgataaata aatccatcct ggtgtcgtct ctaacgacca
    11941 gcatataaca gaaggagcat cagttcttat tttcccatct tttattattt gcaggcctct
    12001 gctcattgtt gtcgcatgaa aaagatttaa tattttattt tcagccttca ctctatttcc
    12061 tcatttccca agaaacatat taaaaacgga aaacattaca cataacctgg aggacaaatc
    12121 atgactttaa atttccctgc cggggaaact acactattct cagcaaaaaa catcctgaca
    12181 gcaatactca ctctgcacaa cttacacgca tagcgttccc atttgaatac agttgcagcc
    12241 cgcgatttat tttgtcttcc ggagccacct ctgccatgaa tatcaaccgc attatttttg
    12301 ccattgaaga ctgtatcagt accctgtcac ggtattccgt ttccgcattc ttccccgatt
    12361 actgtgatct gcacaccgtc atcggtctgg accagcagga ccgcgaacgc cgcccctggc
    12421 tgaaagcccc ctacattgcc accacaaaac aggggtatta cctttccgtc tttgaaatca
    12481 gcggtgccct gaaagaaatg gatgaaaacc cggacctgac agcccccggc acactggaat
    12541 cactgattac agcgctggca gagcgaatga acactgccta taaaaacacc ggtcacaaaa
    12601 tcagttttgt tttcgaacgc gatccggagc gtggaaggaa tgaaatcgaa gccatgctct
    12661 taccccagcg aaaatccatc gcacgcacgg ggatcctgct tcaggacgtt ctggatgaaa
    12721 aagtcaccac gctcaccccc tggcttgtcc gggaacgctg ctggctgacc gtctggagct
    12781 ctgaagaact gctgagccgg accgatcgca aggaccacca gacccgtatc aggaaactcg
    12841 ctgaacacgc tccgccagcc cgatttgcgc aggatccatg gcgctggaca ctttccgcac
    12901 tcaaaatacg gcatgacgcc tttctggaca cgctggagca ggccctgaca catgattctg
    12961 atggtctgct ggttcgcctg atggatattc atgaagtggg acgggaaatc cgccgtcaac
    13021 tggagcgcaa cagcacacct gcagtatggc agcctcatct gcctgaagat gcccgtcctg
    13081 ccggctggcg acagggcgag gatacctctg tattccacgc accgtcgctg aatctgcagt
    13141 tattcaccac acagcctgaa acacacggca gcctgataaa ggccggtgac ctctggcacg
    13201 gtatggtcgc cattaccctg cccccccaga atctgcggac attcaaccag ctggtcaggg
    13261 atgtgccccg cgcaatcccc tggcgtatcc ggatggatct gatgcccggc ggcatgaagg
    13321 cgcttggggt caagaaaacc ctgctgacct acagcagctt tatttcccct ctccggccca
    13381 tgtatgactc agtgatgatg ctgaatgaca tcaacaaaca tgatccggtc tgtatcatga
    13441 ctattgttgc caccacatgg ggaaacagcc gggaagtctg tacccgcaac caggcgctcc
    13501 tgaaatctgc gctggaaggc tggggagtat gcggcaccac caccaccttc ggtgatccga
    13561 gacgtgcctg ggtgaacacg gttctggcgg cctcccacgg ttccggtcca gtcccccttt
    13621 acccgcccct ttcacacgcc ctttccctgt ttcccctgaa cagggccggc tctgtctggc
    13681 ggggacaggg aaatctgatg atgcacaccg aggacgggtc tgcatgggag gtcgcgctgg
    13741 catcgtcaca acagaacaag cacacggaac tgacgcccgg cgcacccggt ctgggcaaat
    13801 cggtactcat caacgcactg tcggagatcc agatagcctc agcccagaag aacctgcctt
    13861 tcattgccta tatcgacaag ggattcagtg cacagggact ggtacagctt atccgtgaca
    13921 gcctgccgga gcagagaaag gacgaagccg tggggatcat tctgagcaat gatccggaac
    13981 acacccgtaa cctgtttgat gtgatgtacg gtgctaaaaa acccgccacc ccggaaaaga
    14041 acttcatggt atctgtactc tgcgcactgt gcgtggatac cggcaccggt cagccctgta
    14101 atcccggtga cacccggcaa atcataagca acctggtgga tctggccttc agggagtatg
    14161 gcgaaaacaa ccccagactg taccgtgcag gaacagagcc ccttgtggat ctcgccctgg
    14221 aagagagtgg catcgcagaa caacatgacg ctggatggtg gaatgctgcc acctggtttg
    14281 aaatcaggga catgctgcat atggctggca atatccccgc agcccagcgg gcacactacc
    14341 aggccatgcc cctgctggct gaaatgtccg ccctgctggg acagccatcc atccgggatg
    14401 tgttcggcac agttcagcgc gataactcag cagaattgtt actggactac atccgccgtg
    14461 ccctcgatca gggacacagt gactacccga tgatgtccgg ctgtacccgg tttatgctca
    14521 gccccgacac ccgcgtcgtg gccgttgatc tgaacaatgt ggccggtgac aagacacctg
    14581 ccgggcgtct gcgcactggc atcatgtacc tgctggcagg acaaatcgcc ggcggggatt
    14641 ttgtcctgcc gcagtatcag gaggaaatac ggaaaaatct cgacccacgc taccatgagg
    14701 tgattttccg ccgcattgaa cagcttgatc aggaggtcaa gaccaaagtc tatgacgaac
    14761 tgcacaatgc aaaagggatc aatttcatct gggaagccct ggacacccag gagctggagc
    14821 agcgtaagtt cgggatacgc acagtgctga gcacccagct actgcaggac tatccaccgg
    14881 cagttctcaa atccgccaac accctctggt tgctgcgtta ccgccctgac gaaattccgt
    14941 ttctgcggga taattttggt gtccctgaag tcaccctgcg gcgctttctg aaaatgccgg
    15001 aaggggctgc accggatggc agcggtgtcc cggtactggc tgtgttccgt gtaaaaaacg
    15061 gtacgctggc ccggatcctg aaattcactc tcggtccact ggagctgtgg gcactgaatt
    15121 cctcacccaa agacagcgcc ctgagacgtg cgctgacgca ggagctcggg tcactgcggg
    15181 cacgccagat cctggcagaa aactttcccc gtggttcagc cacctcactg atagaacacc
    15241 gcgcccgtac acatgacagt gaaaacgtca tccatgatct ggctgcagaa ctcatccgta
    15301 agcagggata tcacctctga tgtacagcat acacatcact ccccgctgcc ggcgtcctgc
    15361 acggggacag gtcttcctga aaacgctgcg cgtggacggt gacagtttca gcttcaccct
    15421 gccgtccgcc tgcttcacgc tgcgtccctc ccccaatccc cgggtcattt tcgccagtga
    15481 cagacgggct gacggggaaa tgagtggcgt tcgcctggct ctgcccggag agacaggcac
    15541 gctgattaca acggccacct ggcaggtcaa cgtgccggag cggcctcctt ttgatataca
    15601 gcatgtaatt cactggctcc tgccctcgcc tctcacggcg ctgatgtccg acaacacctg
    15661 gctgtggccg gatagccccg gcacaccggc actgacggcg ggagactggc cggtaatgga
    15721 aaccgatacc ccgacgctga aacgcgtgaa gcgtcgtacc ttcacgctca tcgataaccg
    15781 caaccgtcag ggatggatca cccgtcgctt ccagtgcttc ccgctggctc cgctgccgga
    15841 atcacacagt caccgcgtac cagacatcct gtatcccctg aaaacggaga aaaaagaatg
    15901 caaagacaac cagcaaccac actgcttgca ctcctggtag gctgtgcggt tatcccgtcc
    15961 acccacgcct attctgtgac cgtagtggcc agccagcctg tcaccacaca aatcatgccg
    16021 gagctggccg ccattcaggg cgtgatggca gagatggtcc gtatgcagac agccaccggc
    16081 acagccatca accagagttc agagaagctg gcctctgtca tcgcacagga tgggcaggcc
    16141 acccgtcagc agatgatatt cagtaatgaa acccgccggc ttgaggaagc ccggaagagc
    16201 ttcagcgtgc cggactccat ctgcagtgaa tccgcctcag gagttgcagc tgaaagtcgc
    16261 cgtgcagcag cgtctgcggc tgcacgtctg agtcagggag gaggcgtcag cagtaaaccc
    16321 atccgtgaac gtctctcccg ggcagcagac tcaccaaccc gcgaggcata tgacagtgca
    16381 ggcattcatg ccggttactg cacggaagct gaatatgccc gctttggcgg aaccgatgtc
    16441 tgccctgccg tgggcgagct gccgggcgga gacagccagg tgcgatcgct ctatcagggt
    16501 gcaggcacag cagatagccc tgccgccctc acctgggatc agaaacagat tgatgctgcc
    16561 acggcttaca tgaaaaacac cgccagaccg tcagcgggtc gtgccccggg gaaaggtgaa
    16621 gtgggaacac agaccgggcg cacgtatgtg ggcctgcaga atgaatacaa cggcatcatc
    16681 gatgccgctt ctcatccgca actgtcactg atagctgaca gtacccctaa tgaggccacc
    16741 cgtggagcgc tgactgaagc cctgcagtca ccatccgctg cggcatattt tgaccggacc
    16801 gcatcatcag aggcccgcac ccgtggtcac atgagccagc gggagtttga ggcctttgaa
    16861 gctggcagac gctatgcaaa caccgactgg cagcaggatc tgcagggcat ggaaggcgat
    16921 aaccttctcc gtgaactgct gcgcaccacc gccctgctga actggcagat gaacgacctg
    16981 aaagagcaga tacgccaggg taatgttatc gccgggcagc agctggcact ggcggcacga
    17041 cagtattacg gacagcgtct cggtgaactg tcacaggcca tgagccaggg gagcgtaaga
    17101 tgaaagacga caacagcacc gacaaaaaaa aaccggcccc ccgtctccgc cgcatcggtg
    17161 acatcgggat aaacctgttc gcaccactgg cagaaacccg gtggatgctg cgcagctgcc
    17221 gccatatgct gggccagcat aaaacacgcc tgaagcgcct catgcccgac cggcaaccgg
    17281 agagtccggt ctgtatgacc tgggaagaag ccgtcagggc cagtggcctt acggtcaacg
    17341 aactggatcg gcgtctgcga cgtcgcagga tgttctggcg gacaggattc tggctgatga
    17401 tgataccggt acttctgctg tgcggcatgg cccttgctgc cacagcgctt ccggccatca
    17461 ccctgatacg tatactggcc tgcctgttac tgatgagtgc cattgcagca ctgtgtgcct
    17521 caaaaaccct gattgtgtca taccgcctct ggcaactgaa tgaacggcgg gtcagcgagg
    17581 acgaacaggg caccttcccc gattacctgc aggcccggca cggctggaaa caggctacat
    17641 ggctggccat tacccaccgg cagtactgac cgtccggtct gcaattcctt ttcctcattc
    17701 cggaggctga tgccctgcat cccttcggtt ccggagttat cgtgaaaatc atacccagag
    17761 cactgagtgc gggtctggcg ctggctgccg tacctgcaca ggcattcgtc acctatcagg
    17821 acattgtgag cgccgccacc aatccggatg acctgtcgcg ccaggcactg gtcaccattt
    17881 ttggcgacgt ggtcacaaat cccctgacag ccagcagcaa caccattatc ggtaacctgt
    17941 tcatggtgtt taacgcaatt atcgccatta tggggatagt ctggttcctg tttattggaa
    18001 tccgccatgt tgtccgcacc ggccaccagg gcaatgtctt taacaacgga cgggacatcg
    18061 tcggaatgct cagtattgtg agcggtttta tgatgattgt gcccaccgct tccggctgga
    18121 gcctggcaca actggtcatg ctctggggcg catccatcat gggcattggt tccgccaata
    18181 tcatggttca gactgctgca gaaaacatcg ctgaaggcta ttcaatgacc gtacagccag
    18241 ttcatgtctc cacccgcacg gcagcacggg gaatactgga gatggcgctg tgcaaacacg
    18301 ccatgaatgc aggcctcgat gactttaacc gtactgcccg ttcatcaacg ccccccatga
    18361 gcgaatcctc acggacagag aacggcaggt ataccgtcac cgtttcaaat ggcagtgcca
    18421 cctgcgggag tgtgagtctc accgtgaaag ggaatggcag caacaaccag tccacactgg
    18481 actatttttt caatcccttc attgaaacgg agtatcaggg tgtcctgacg gcacaacgct
    18541 ccgccatgga taccatgctc cgtgacctgg acagcgcagc acaagaattt gtgtctgcgt
    18601 accggacccg acgcgacacg ggtaacggca acctgccgga tattgaaagc cgtattcaga
    18661 aggctgccaa ccggtatgaa gaaacggtgc agaaggcgtt gccaacagat aacgcagaaa
    18721 ctgagcgcag ggagcagctg aaatcctacc tgaccaccta cggctgggtt gccctggggg
    18781 cctggtacca gacctttgcc accgccaacc agcgactggc agaactggcc gaccgtgccc
    18841 ccactgtgag tggagtttca gcgcagggcg aagccggaga tactgagttc ctgaaggaag
    18901 tcaaaggagc ttaccaggca cagttgcaga actcaccata tacacctgca ctgggaacag
    18961 ccacctcagc cagtgaatac caggcatcta atacacagaa tcctcaatca gccctgttaa
    19021 aactcctagg gaatgacctg acgttattta ccaatcaaat tgcaaccata cattcaggaa
    19081 ctggaaccac ctcggaccaa ctaaatccgc taataaaaat gaaaaacatt ggtgattaca
    19141 ccatggtcac aaccgaaacc atctgggcgg gttataccac cgtcagggtg ctttcctctg
    19201 ccggcagcaa ctccattttt ggtcgactgg tcaatgccgt tagcggctta tctgacgccg
    19261 caaaggcttt tctggaagca tccgcaccac cgctctactt cctgctgttt ttgctgttct
    19321 gtgccggttt ttcgttatcc atttacctgc cgtttattcc gtttatcttc tggatgaccg
    19381 gcgtcggtaa ctggatagtc agtgtactga ttggatgcgc cgccggcccc ctgtgggccg
    19441 caacccatct gggaacaagc caggacaggg gaagccgtgc tgcttatggt tacatctacc
    19501 tgatagacag tatgatcaga ccactgctga tggtgttcgg gttctttttt gcatcggtgg
    19561 ccatcatggc cggcggcaca atactgaatg cgctgtttgc cgccgcgctg atgaatgtcc
    19621 agataaattc tgtgacgggc gtgttcagca tggcaggatt tttgatgatt tatgcccgcg
    19681 tctgtaccac gctggttgcc gccgtttttg cacttcaggc ttatctgcct gaccatgtaa
    19741 ttaattttct gggcggacgt gacggcgtta atatgctggg gaatatggtt tcttcagtca
    19801 aagatattat tgccggaggt aataccaata tgcgcagaac acctggcgtt cgagaagaaa
    19861 aactgaaaaa tattaatgga gatgataagg acggaataaa acaatagagt aaaaacatca
    19921 aaaataaata aagcagtatt gccatattca cctgaagaga ttaaaatata ttcaggtgaa
    19981 tatgggggtt tattatgaac aaaacaaaaa gcagatatcc ttcctggtgg gcatattatc
    20041 aactagcccg caacgcttat tttttattag gcattccgtg cttagcttta tacgcaacag
    20101 cttatacatc atatttatta gatgataaac taacgcattc aacaagcctt tttattctgg
    20161 catatggatg ttattttctt cttttcattc ctccgctctg gctatatctg agaaccagag
    20221 cgaaaaaaaa gaaagtccag caggttgttc agcagataaa agaaagcagt ccctttgcgc
    20281 cgactagcga ttacgagcaa ctgtcattct cgaagagttg ttatttcggg atcgacatta
    20341 aaaacggcac catgctgtat gtgcgcattt acccaaacaa tgtcatggat gtgattggtc
    20401 tggatattca caacttcacc cgaacagttg ctgaagacgg caagctggaa atccacacca
    20461 cctatgtgag tctgccgatg atccctcttg aaatctccgg catctcagca aggacgctgg
    20521 ccaacaccat acacaccatg gctgcacgtg gatatgagta caaagagcgt ttcccgcaga
    20581 tgattcggta tcgggttaaa gaatgggaaa aagttgccgg tgtgcctgtt gcagaagtgt
    20641 tttaatacgg ctcatacatc cggttcatct gaaccggatt ttctttacta caacagataa
    20701 cttatatcgt tatcgataaa gtcgcccaag agccttgata gctgcaactt taatctccat
    20761 atcatcactt tcacttaatc tacgtaattc cctctttatg ttatctgcct ggcatcgacc
    20821 ttcacctaat gcatagatca cagcaagtgt aacgtctttc gatttatcct tcatcatttc
    20881 aagcaagaaa gtattagcta tcgcaggaga catactcata ataatttcct ctttataaaa
    20941 ttaacaaaga tactcattta gatgtataaa caccgtataa taaaataatt cttgctaaac
    21001 aagaaattaa aatcttgcat ccacagtctc gcacaccatg aagacactcg gatatgagta
    21061 caaggaacgt ttcccgcaga tgatccggta tcgggttaaa gagtgggaaa aagttgccgg
    21121 tgtgcctgtt gcagaagtat tttaaggtag cgaccaactg ttagacggta atccgtcagc
    21181 cggttttctc tgaaccggct ttttcttttc aggcaaaatg cctgtctgcc atccctgtat
    21241 cagactgtaa ttagcacaat acggcaattt gccgttcagt atattccagg aggtacaaat
    21301 catgccatct gcaacacaaa ctgcccgact ggaagcaagg atcagcacag aactgcatgc
    21361 tatgctgaaa cgcgctgcag aaatccaggt aaaaaagggc gcattgaggt ttgctttcgc
    21421 aacaatccgg cggcagattt cctgccgccg tgttgcccgt taacgtttaa cttcgtaggc
    21481 taacgttgcc acaagcactg tgtttccctg ctcgacacgg aatccgcaaa gcgaatccct
    21541 caccatccag acaaaacaca gtatcgtcac acagacgaca attaacattg ttaaaaacgt
    21601 tcgttgtggc atcgcagccc ctccattgcc tttcggccgt catcgggcta ctataatgtt
    21661 gttgaggcat tacagtg
//
LOCUS       DAZGSP010000085        21561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZGSP010000085 DAZGSP010000000
VERSION     DAZGSP010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.085"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            82..1596
                     /gene="caiT"
                     /locus_tag="ACHK74_003566"
     CDS             82..1596
                     /gene="caiT"
                     /locus_tag="ACHK74_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFP0222315.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1627..2769
                     /gene="caiA"
                     /locus_tag="ACHK74_003567"
     CDS             1627..2769
                     /gene="caiA"
                     /locus_tag="ACHK74_003567"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFP0222316.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2897..4114
                     /gene="caiB"
                     /locus_tag="ACHK74_003568"
     CDS             2897..4114
                     /gene="caiB"
                     /locus_tag="ACHK74_003568"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFP0222317.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLSTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4188..5741
                     /gene="caiC"
                     /locus_tag="ACHK74_003569"
     CDS             4188..5741
                     /gene="caiC"
                     /locus_tag="ACHK74_003569"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFP0222318.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5850..6635
                     /gene="caiD"
                     /locus_tag="ACHK74_003570"
     CDS             5850..6635
                     /gene="caiD"
                     /locus_tag="ACHK74_003570"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFP0222319.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6641..7231
                     /gene="caiE"
                     /locus_tag="ACHK74_003571"
     CDS             6641..7231
                     /gene="caiE"
                     /locus_tag="ACHK74_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFP0222320.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7317..7712)
                     /gene="caiF"
                     /locus_tag="ACHK74_003572"
     CDS             complement(7317..7712)
                     /gene="caiF"
                     /locus_tag="ACHK74_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFP0222321.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7973..11194)
                     /gene="carB"
                     /locus_tag="ACHK74_003573"
     CDS             complement(7973..11194)
                     /gene="carB"
                     /locus_tag="ACHK74_003573"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFP0222322.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11212..12360)
                     /gene="carA"
                     /locus_tag="ACHK74_003574"
     CDS             complement(11212..12360)
                     /gene="carA"
                     /locus_tag="ACHK74_003574"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFP0222323.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(12816..13637)
                     /gene="dapB"
                     /locus_tag="ACHK74_003575"
     CDS             complement(12816..13637)
                     /gene="dapB"
                     /locus_tag="ACHK74_003575"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFP0222324.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            complement(13804..14718)
                     /gene="rihC"
                     /locus_tag="ACHK74_003576"
     CDS             complement(13804..14718)
                     /gene="rihC"
                     /locus_tag="ACHK74_003576"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFP0222325.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            complement(14784..15734)
                     /gene="ispH"
                     /locus_tag="ACHK74_003577"
                     /gene_synonym="lytB"
     CDS             complement(14784..15734)
                     /gene="ispH"
                     /locus_tag="ACHK74_003577"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFP0222326.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(15736..16185)
                     /gene="fkpB"
                     /locus_tag="ACHK74_003578"
     CDS             complement(15736..16185)
                     /gene="fkpB"
                     /locus_tag="ACHK74_003578"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP0222327.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(16273..16767)
                     /gene="lspA"
                     /locus_tag="ACHK74_003579"
     CDS             complement(16273..16767)
                     /gene="lspA"
                     /locus_tag="ACHK74_003579"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFP0222328.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(16767..19583)
                     /gene="ileS"
                     /locus_tag="ACHK74_003580"
     CDS             complement(16767..19583)
                     /gene="ileS"
                     /locus_tag="ACHK74_003580"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFP0222329.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(19626..20567)
                     /gene="ribF"
                     /locus_tag="ACHK74_003581"
     CDS             complement(19626..20567)
                     /gene="ribF"
                     /locus_tag="ACHK74_003581"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFP0222330.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(20575..20793)
                     /gene="yaaY"
                     /locus_tag="ACHK74_003582"
     CDS             complement(20575..20793)
                     /gene="yaaY"
                     /locus_tag="ACHK74_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFP0222331.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            20896..21159
                     /gene="rpsT"
                     /locus_tag="ACHK74_003583"
     CDS             20896..21159
                     /gene="rpsT"
                     /locus_tag="ACHK74_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFP0222332.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         5133 a         5790 c         5536 g         5102 t
ORIGIN      
        1 taataattat attaaatgtt aacaaaaata aaacaaacgg gaacgcaaaa taaatattcg
       61 ttttcacagt ggaattaact catgaagaat gaaaagagaa aaacgggaat agaaccgaag
      121 gttttctttc cgccgttaat aatcgtcggc atactttgtt ggcttacagt cagagatctg
      181 gatgcagcga atgtcgttat taatgctgta ttcagttacg tcaccaatgt atggggatgg
      241 gcatttgaat ggtatatggt ggtgatgctt ttcggttggt tctggctggt gtttggcccg
      301 tatgccaaaa agcgtttagg taacgaacca cctgaattta gtaccgccag ttggatcttt
      361 atgatgttcg cctcctgtac gtctgctgcc gtactgttct ggggatcgat tgagatctac
      421 tactacatct ccaccccgcc gtttggctta gaaccgaact cgacaggggc gaaagagttg
      481 gggctggctt acagcttgtt ccactgggga cctctgccgt gggccactta cagcttcctt
      541 tcagtcgcct tcgcttactt cttctttgtc cgcaaaatgg aagtgattcg ccccagctcg
      601 acactggtgc cgctggtagg tgaaaaacac gccaaagggt tgttcggcac tatcgtcgac
      661 aacttctatc tcgtcgcctt gatcttcgcg atgggtacca gtctgggcct tgccacgccg
      721 ctggtgaccg agtgtatgca atggttgttt ggcattccgc ataccctgca actggacgct
      781 atcatcatta cctgctggat tatcctcaac gccatttgcg tcgcttgcgg tctgcaaaaa
      841 ggggtacgta tcgccagtga cgtgcgtagt tacctgagct tcctgatgct gggttgggtg
      901 ttcattgtca gcggtgccag cttcatcatg aactacttca ccgattcggt ggggatgttg
      961 ctgatgtatc tgccgcgcat gttgttctat accgatccca tcgctaaagg cggcttcccg
     1021 cagggctgga ccgtgttcta ctgggcatgg tgggtgattt atgctatcca gatgagtatc
     1081 ttcctcgccc gcatctcccg tggtcgtact gtgcgtgaac tgtgcttcgg catggtgctg
     1141 gggctgacag cgtcaacctg gatcctgtgg actgtactcg gtagtaacac tctgctgttg
     1201 atagataaaa acattatcaa cattccaaat ctgatcgaac agtacggtgt ggcgcgcgcc
     1261 attatcgaaa cctgggccgc tctgccactc agcaccgcca ccatgtgggg cttcttcatc
     1321 ctctgcttta ttgccaccgt tacgctggtt aacgcctgct cttataccct ggcgatgtcc
     1381 acttgccgcg aagtacgcga tggtgaagaa ccaccgctgc tggtgcgtat cggttggtca
     1441 attctggttg gcattatcgg tattgttttg ctggcgctcg gcggcctgaa accgattcaa
     1501 actgccatta tcgccggagg atgcccgctg ttcttcgtca acattatggt gacgctctcc
     1561 tttattaaag acgcgaaaca gaactggaaa gattaattaa cccccaaaat atcaagaggt
     1621 tgaaagatgg attttaattt aaatgatgag caggaactgt ttgtcgccgg tatccgcgaa
     1681 ctgatggcca gcgaaaactg ggaggcctat tttgccgagt gcgaccgtga cagcgtctac
     1741 ccggaacgtt ttgtcaaagc actggcggat atgggtatcg acagtctgct gatccctgaa
     1801 gagcacggtg gtctggacgc ggggtttgtt actctcgccg ccgtgtggat ggagctggga
     1861 cgtctggggg caccaaccta tgtactgtac cagttgccgg gcgggttcaa caccttcctg
     1921 cgcgaaggca cacaagagca gatcgacaag attatggctt tccgcggcac cggtaagcag
     1981 atgtggaact cagcgattac cgaaccgggc gcgggctccg acgtgggtag cctgaaaacg
     2041 acttataccc gtagaaatgg taagatttat cttaatggta gtaagtgttt tattaccagc
     2101 agcgcctaca ccccgtacat cgtggtgatg gcgcgcgacg gggcttctcc ggacaaacct
     2161 gtctacaccg aatggtttgt tgatatgagc aaaccgggca tcaaagtgac caaacttgaa
     2221 aagctcggtc tgcgtatgga tagctgctgt gaaatcacct ttgacgacgt ggaactggac
     2281 gagaaagaca tgttcggtcg ggaaggtaac ggctttaacc gcgtcaaaga agagttcgac
     2341 catgaacgtt tcctggtagc cctgaccaac tacggtacgg cgatgtgcgc ctttgaagat
     2401 gcggcgcgct acgccaacca gcgcgtgcag tttggcgagg ctattggtcg tttccagttg
     2461 attcaggaaa aattcgccca catggcgatc aaattaaact ccatgaaaaa catgctgtat
     2521 gaagcagcgt ggaaagcaga caacggcacc atcacctctg gcgatgcagc gatgtgcaaa
     2581 tacttctgcg ccaatgcggc atttgaagtg gtggatagcg caatgcaggt gctgggcggt
     2641 gtcgggattg cgggcaacca ccgcatcagc cgcttctggc gtgacctgcg tgtagaccgc
     2701 gtctccgggg gatctgacga aatgcagatc ctgacgctgg gtcgcgcggt gctgaagcaa
     2761 taccgctaag tccgtgcgct gcctgatgcg acgctaatgc gtcttatcag gcctacggga
     2821 gcacgaatgt aggccggata aagcgtttac gccgcatccg gcagtcatgc gctacataat
     2881 ttttcaggag ctaataatgg atcatctacc catgccgaaa ttcgggccgt tggccggatt
     2941 gcgcgttgtc ttctccggta tcgaaatcgc cgggccgttc gccgggcaaa tgttcgcaga
     3001 atggggtgcg gaagttatct ggatagagaa cgtcgcctgg gccgacacca ttcgcgttca
     3061 accgaactac ccgcagctct cccgccgcaa tttgcatgcg ctgtcgttaa atattttcaa
     3121 agatgaaggc cgcgaagcgt ttctgaaatt aatggaaacc accgatatct tcatcgaagc
     3181 cagtaaaggt ccggcctttg cccgtcgtgg cattaccgat gaagtactgt ggcagcacaa
     3241 cccgaaactg gttatcgctc acctgtccgg ttttggtcag tacggcaccg aggagtacac
     3301 caatcttccg gcctataaca ctatcgccca ggcctttagt ggttacctga ttcagaacgg
     3361 tgatgttgac cagccaatgc ctgccttccc gtataccgcc gattactttt ctggcctgac
     3421 cgccaccacg gcggcgctgg cagcactgca taaagtgcgt gaaaccggta aaggcgaaag
     3481 tatcgacatc gccatgtatg aagtgatgct gcgtatgggc cagtacttca tgatggatta
     3541 cttcaacggc ggcgaaatgt gcccgcgcat gagcaaaggt aaagatccct actacgccgg
     3601 ttgcggtctg tataaatgtg ccgacggcta catcgtgatg gaactggtgg gcattaccca
     3661 aattgaagag tgctttaaag atattggcct cgcacatcta ctcagtacgc cggaaatccc
     3721 ggaaggcact cagcttatcc accgtatcga atgcccttac ggcccactgg tagaagaaaa
     3781 actcgatgcc tggctggcgg cacataccat cgcagaagtt aaagaacgct ttgccgaact
     3841 gaatatcgcc tgtgccaaag tactgaccgt accggaactg gaaagcaatc cacagtatgt
     3901 tgcccgtgaa tcaatcactc agtggcaaac gatggatggt cgcacctgca aagggccgaa
     3961 catcatgccg aaattcaaaa ataaccccgg acaaatctgg cgcggaatgc cctcacatgg
     4021 catggacacg gctgccattt tgaaaaatat cggctacagc gaaaacgaca ttcaggagtt
     4081 ggtcagcaaa ggtctggcca aagttgagga ctaaacgcgt ggcgtcagtc ggcttgcctg
     4141 actagcgccc gcccaataac aaacgaacaa aaatggatag aggtgcaatg gatatcattg
     4201 gcggacaaca tctacgtcaa atgtgggacg atcttgcgga cgtttacggt cataaaacgg
     4261 cgctgatttg tgaatccagc ggcggagtcg ttaaccggta tagttatctt gagttaaatc
     4321 aggagattaa ccgcacggca aacctgtttt atacgctggg gattcgcaaa ggcgacaagg
     4381 ttgcactaca tctcgacaac tgcccggaat ttatcttttg ctggttcggg ctggcaaaaa
     4441 ttggcgcgat tatggtgccg attaacgccc gcctgttacg cgaggaaagc gcgtggatcc
     4501 tgcaaaatag ccaggcgtgc ctgctggtga ccagtgcgca attctatcct atgtatcaac
     4561 agattcagca ggaagatgcc actcaattgc ggcacatttg cctgacagat gtggcacttc
     4621 ccgctgatga tggcgtgagt tcgtttactc aactgaaaaa tcaacaacct gccaccttgt
     4681 gctatgcacc gccgctatcg actgacgata cggcggaaat tctcttcacc tccggcacca
     4741 cctcccgacc gaaaggtgtg gtgattaccc attacaacct gcgcttcgct ggatattact
     4801 ccgcctggca gtgtgcactg cgtgacgatg acgtctacct gacggtaatg cctgcgtttc
     4861 atatcgattg ccagtgtact gcggcgatgg cggcgttttc tgccggggcc acctttgtgc
     4921 tggtcgagaa atacagcgcc cgcgccttct ggggacaggt gcagaagtac cgcgccacca
     4981 ttaccgaatg tattccgatg atgatccgta cgttgatggt gcagccgcct tcagcgaacg
     5041 atcggcaaca ccgcctgcgg gaagtgatgt tttatctcaa cttgtcggag caggaaaaag
     5101 atgcgttttg tgaacgcttc ggcgttcgct tgctgacgtc ttatgggatg acggaaacca
     5161 ttgtgggcat tatcggcgat cgccctggcg ataaacgacg ctggccgtcg attggtcggg
     5221 cggggttttg ctacgaagcg gagatccgcg acgatcacaa tcgcccgctc ccggcaggtg
     5281 agatcggtga aatctgtatt aaaggcgtac cagggaaaac catcttcaaa gagtattttc
     5341 tcaacccgaa agccactgcg aaagtgctgg aagccgacgg ctggctacat accggcgata
     5401 ccggatactg cgacgaagag ggcttttttt atttcgtcga tcgccgctgc aatatgatca
     5461 aacgcggtgg cgagaatgtc tcctgcgtgg agctggaaaa tatcatcgcc acccatccaa
     5521 aaattcagga tatcgtggtt gtgggtatta aagattcgat tcgcgatgaa gccatcaaag
     5581 catttgtggt gctgaatgaa ggtgaaacat tgagcgaaga ggaatttttc cgcttctgcg
     5641 aacaaaatat ggcgaaattt aaagtgccct cttatctgga gatcagaaaa gatctgccac
     5701 gtaattgctc ggggaaaata attagaaaga atctgaaata aatgaaacag cagggaacta
     5761 ccctgccagc taataatcac gctataaaac aatatgcatt ttttgcaggg atgttgtcgt
     5821 ccctgaaaaa gcaaaaatgg agaaaaggaa tgagtgaatc attacatctg acccgcaatg
     5881 gatcaattct ggaaattacc cttgatcgtc caaaagcgaa tgctattgat gcaaaaacca
     5941 gctttgaaat gggcgaagta tttctaaatt tccgtgacga tccgcaatta cgtgtcgcca
     6001 ttattaccgg tgccggagag aagttctttt ccgcgggctg ggatttaaaa gcggcagcag
     6061 aaggcgaagc accggatgct gactttggcc cgggtggatt tgcgggatta accgaaattt
     6121 tcaatctcga caaaccggtt atcgcagctg tgaacggcta tgcctttggc ggcggtttcg
     6181 aactggcgct ggcggcagat tttattgttt gtgccgataa cgccagcttc gccctgccgg
     6241 aagccaagct gggcatcgtt cctgacagcg gcggcgtgct gcgtctaccg aagatcctgc
     6301 cgcctgccat cgtcaatgaa atggtgatga ccggcagacg aatgggcgca gaagaggcgc
     6361 tgcgttgggg gatagtcaac cgcgtggtta gccaggcgga actgatggat aacgcccgcg
     6421 aactggcgca gcagctggtt aacagcgccc cgctggcgat tgcggcgctg aaagagatct
     6481 tccgcaccac cagcgaaatg ccggtagaag aagcgtatcg ctatattcgc agcggcgtgt
     6541 tgaaacacta tccgtcggtt ctgcattcgg aggatgccat tgaagggccg ctggcgtttg
     6601 ccgagaagcg cgatccggtg tggaaaggac gttaacaacc gtgagctatt acgcctttga
     6661 ggggttaatt ccggtggttc acccgacggc gtttgtccat cccagtgccg tcttgattgg
     6721 cgatgtgatt gtgggagccg gtgtctacat cggcccactc gcctcactgc gtggtgacta
     6781 cgggcggttg atcgtgcaag cgggagccaa tattcaggat ggctgcatta tgcatggcta
     6841 ctgcgacact gacaccatcg ttggggaaaa cggccatatc gggcacggag cgatcctgca
     6901 tggttgtgtg attggtcgcg atgcattggt cgggatgaac agcgtgatta tggatggcgc
     6961 ggtcattggc gaagagagca ttgttgccgc catgagcttt gtcaaagcag gctttcacgg
     7021 cgagaaacgc cagttgttga tgggtacgcc agcccgcgct gtacgcagtg ttagtgacga
     7081 cgagttacac tggaaacggt tgaataccaa agagtatcag gatcttgtcg ggcgctgtca
     7141 tgcatcgtta catgaaacac agccactgag gcaaatggag gaaaatcgcc cccgtttgca
     7201 ggggacgacg gatgtgacgc ctaaacggtg atcttgccgg atgcgacctg gttgctggat
     7261 aagatgctac aacatcgcat ctggcaagca ggacgcccgg tatcaacgag ataaaattaa
     7321 cgacgcatac tctttgacaa catcatctgc cacttctgct cgcggttaag ttccgtgagc
     7381 ggaacggcag gaatacgcgg cgtctttctt acgccgacta atttatcccg gctgttattg
     7441 cgcaggcgcg cgtaaatttg ctcatcgata tcgaccactt ttaccagtcg ctggcactgg
     7501 catccccgtc cttccagctt attagggatc atcttaactt cgcagcttat ttctgcgact
     7561 tccgacagaa tataagtcag ggtattaacc gccttgctgt gttcaatatc gaaatgaata
     7621 gagatctctc ttgctatcac ccaccgattt tcagccatca tccattcggc gattaacaag
     7681 tagagtggtt tttcaacata tccttcacac atacgaactc cagtttcaca aattaaaagt
     7741 ctggctatga ctcgttttga agcaattaaa caaggccata ttaaaattgt ggatatatcg
     7801 atagtatgca acttcaccgt cgataagtgt gagctaaatc ctgcttatgt catcaaaaat
     7861 atcgattccc caaaagattt acttaatggg caaaaaaaac ggtcctcatc agagaaccgt
     7921 attacttatt cgatcaaatt agcggatgaa aaatatccgc catgacacgc tattatttga
     7981 tctgcgcgtg catttcctgc accgaaatta ctttttcggt cgcgtcggca ttcagcgcca
     8041 tcgcggtagc gaaaccaccg ttcagggtgg tgtcataatg cactttatat tgcagcgcac
     8101 tgcgacggat cacgcgggag tcttcaatcg cgcgacggcc tgaagtggtg ttgatgatgt
     8161 aggtatattc gccattcttg atgcggtcct gaatgtgcgg acggccttca tgcaccttgt
     8221 tcaccagacg cggattaatg cccgcttcgc ccagcacaat cgccgtgccg tgggtcgcat
     8281 ccagctcgaa gccctgtttc agcagttttg ccgccaggtc caccacgcgt tctttatcgc
     8341 cttcgcgcac ggaaagcagc gcacgaccgt gtttcttcat ggtggagttg ctgcccagct
     8401 gcgctttggc aaacgcttca gcgaaggtgc ggcccacgcc catcacttcc ccggtggagc
     8461 gcatttctgg ccctaacagc gggtcaacac ccgggaattt attgaacggc agcaccactt
     8521 ctttcaccga gtagtacggc gggataactt ctttggttac gccctgctca gccagcgatt
     8581 tgccagccat cacacgcgcc gccacttttg ccagcggtac gccggtagct ttggagacga
     8641 acggaacggt acgcgccgca cgcgggttaa cttcaatcag gtagacttcg ttgtttttca
     8701 ccgcaaactg cacgttcatc agaccgcgca cctgcaattc gaaggccagt ttctgcacct
     8761 gctggcgcat cacatcctga atttcctgac ttaaggtgta ggctggcaga gaacatgcgg
     8821 agtcaccgga gtgcacgccc gcctgctcga tgtgctccat gatgccgcca atcagcacca
     8881 tttcgccgtc gcagatggca tccacgtcaa cttctaccgc gtcatcaagg aagtgatcca
     8941 gcaacactgg cgcatcgtta gacacgctga ccgccgtctg gaagtagcga cgcaggtcag
     9001 cttcgtcata gacgatttcc atcgcccgac cgccgagaac gtaagacgga cgtaccacca
     9061 gcgggtagcc aatctctttc gccttctcta ccgccatttc aatagcggta acggtggcgt
     9121 tcgccggttg tttcagtttc agacgctcaa ccgcatgctg gaagcgttca cggtcttctg
     9181 cacggtcgat agcatccggg ctggtgccga taaccggtac gccagcagct tccagcgcgc
     9241 gcgccagttt cagcggggtc tgaccgccgt actgaacgat aacgcctttc ggcttctcaa
     9301 tacgcacgat ttctagcaca tcttccagag ttaccggctc gaagtagagg cgatcggaag
     9361 tgtcgtagtc ggtggagacg gtttccgggt tacagttaac cataatggtt tcgtaaccgt
     9421 cttcgcgcag cgccagcgag gcgtgtacac agcagtagtc gaattcgata ccctgaccga
     9481 tacggttcgg gccgccgccg agaaccatga ttttttcacg gtcggtagac ggattcgctt
     9541 cgcactcttc ttcataagtg gagtacatgt aagcggtgtc ggtggcgaac tctgccgcac
     9601 aggtatccac gcgcttataa accgggtgca ggtcatactg gtcgcgcagc ttgcggattt
     9661 ccgcttcgcg cacgcccgcc agttttgcca gacgcgcatc ggcaaagcct ttgcgtttca
     9721 gctggcgcag gaattcagcg ttcaggccag tgatgcccac ttccgctact ttctcttcca
     9781 gacgcaccag ttcttcaatc tgtaccagga accagcggtc aatattggtc aggttgaaca
     9841 cgccgtccac agacaggccc gcacggaagg catcggcgat gtaccagata cgctctgcgc
     9901 cagcatcttt cagttcgcga cggattttgg ttaacgcttc cgggtcatcg aggctcactt
     9961 tcgggtcgaa tccagtcgca ccgacttcca ggccgcgcag cgctttttgc agggattcct
    10021 gctgcgtgcg accaatcgcc atcacttcgc caaccgattt catctgagtg gtcagacggt
    10081 cgttagcacc agcgaatttt tcaaagttga agcgaggaat tttagtaacc acatagtcga
    10141 tggacggctc gaaggaggcc ggagtacgtc cgccagtgat gtcgttcatc agttcgtcga
    10201 gggtgtaacc caccgccagt ttcgccgcca ctttagcaat cgggaaaccg gtcgctttcg
    10261 acgccagcgc cgaagaacgg gacacgcgtg ggttcatttc gataacaatc aggcgaccgt
    10321 ttttcgggtt caccgcaaac tggacattgg aaccaccggt ttcaacgccg atttcacgca
    10381 gcaccgccat cgaggcgtta cgcatgattt gatattcttt gtcggtcagc gtttgggctg
    10441 gcgcgacagt gatggagtca ccggtatgga tgcccatcgc atcaaagttt tcgatagagc
    10501 agacgatgat gcagttgtcg tttttatcac gcaccacttc catctcgtac tctttccagc
    10561 cgatcagcga ctcatcaatc agcagctctt tggttgggga gagatccaga ccgcgggcgc
    10621 aaatttcttc aaactcttcg cggttataag cgataccgcc gccgctaccg cccatggtaa
    10681 aggatgggcg aataatgcac gggaagccca cgtcagcggc aaccgccagc gcttcttcca
    10741 tcgtgtgtgc gataccggaa cgcgcggttt ccagaccaat tttcttcatc gctacgtcga
    10801 aacgacggcg gtcttctgct ttatcaatcg catcggcggt cgcaccaatc atagtcacgc
    10861 cgaactcttc caacacgccc tgacgctcca gctccagcgc gcagttcagc gccgtctgac
    10921 cgcccatcgt tggcagcacc gcgtccgggc gctctttttc aataatcttg cgtactactt
    10981 cccagtgaat cggctcgatg taggtcgcat cggccatttc cgggtcggtc atgatggtcg
    11041 ccgggttgga gttcaccaga atgacgcggt aaccctcttc gcgcagggct ttacatgctt
    11101 gcgcgccaga gtagtcaaac tcacacgcct gaccgataac aatcgggccc gcacccagaa
    11161 tcaggatact ttttatatct gtacgttttg gcatggctct tttactcctg attacttagc
    11221 ggttttacgg tactgctcaa ttaactcgat aaagtggtcg aacaacggcg ctgcgtcgtg
    11281 tggcccaggg ctggcttccg ggtgaccctg gaagctgaac gccggtttat cagtgcgatg
    11341 aatgccctgt aacgtaccgt cgaacaggga tttatgcgtg acacgcaggt ttgcaggtaa
    11401 tgttgcttca tccaccgcaa aaccgtggtt ctgggcggtg atcatcacca cgtttttctc
    11461 cacatcttta accggatggt tgccgccgtg gtgaccaaat ttcattttga cagtcttcgc
    11521 accgctcgcc agcgccagca gctgatgacc gaggcagatg ccgaataccg gaatatcggt
    11581 ttcgaggaat ttctggatgg cggtaatggc gtaatcgcac ggggccgggt cgccaggacc
    11641 gttggagagg aagatgccgt ctggattcat tttcagcaca tcttccgcag aagtttgcgc
    11701 cggaacgatg gtcaggcgac agcctctatc caccagcatc cgcaggatgt tgcgcttggc
    11761 accaaaatca taagccacga cgtggaacgg cagctcgtct tcttttttcg cttctggcag
    11821 gccgccggtc aacgtccagc tcccttgtgt ccagctatag gcttctgcgg tggtcacttc
    11881 ttttgccaga tccatgccat tcagacctgg gaacgcgcgg gctttttcta acgccaacgc
    11941 cgcatccggg ttatcgcctg cgataatgca gccattctgt gcgcctttct cgcgcagtaa
    12001 acgcgtcagc ttacgggtat cgatatcggc aatcgccacg atgttatggc gcttcaggta
    12061 agaagagagg tcttcggtat tacggaagtt gctggcaatc agcggcagat cgcgaatcac
    12121 cagaccttgt gcatgtacct gagaagattc ttcatcggcg tcattagtgc cgacattgcc
    12181 aatatgggga taagtaagag taacgatttg acgagaatag gaaggatcag tgaggatttc
    12241 ttgataaccg gtcattgaag tattgaaaac gacttcccca accgccgaac ctgttgcccc
    12301 tatggcccga ccgtgaaact gggttccgtc ttccagaacc aatagcgctg acttaatcaa
    12361 aacaccctcc agagaatatt cactcacttt atttgcatat taattcacaa tgatgacgta
    12421 aatcaatgca aatctgctta ccgacgaatt tctggcaaac ggcggcattc tggagatatg
    12481 ggcgctaaaa gtcaacttaa tggcgggatt ttttatatta ttttgtggct catgcatgtt
    12541 ttacaggcat taagcgacaa aaagatcata taacctgttc tggaaaacgc ttgcgcagca
    12601 agaagattgc atgaaataaa agcgagcagg gaaaagtgga ccaaatggtc aaaatttata
    12661 ttaaaacaac aaaaaccgaa gacaaaacat gtaatttttg gcacttatag acaatttaaa
    12721 aacataacat taaaaataaa agggccaaaa attaaagccc tttataatca ataaattaaa
    12781 tgtgttattt ttgcaccata acaaatattt tgtggttaca aactattgag atcaagcaca
    12841 tctcgcatat caaaaagacc actttcctta ccactcaacc acaaagccga tcttaccgcg
    12901 ccgttagcaa atgtcatgcg gctggacgcc ttatgggtga tctccagacg ctcgccaata
    12961 tcggcaaaca tcgcggtatg ttcaccaacg atgtcacctg cacgcacggt ggcaaaacca
    13021 atggtgccag gcacacgttc accggtgtgg ccttcacgac tgtagaccgc gcaatctttc
    13081 agatctttat caagggcgtg ggcgatcgcc tctcccattg ccagtgcggt gcctgacggc
    13141 gcatcaactt tatgtctatg atgtgcttca ataatttcga tatcggtgta gtcacccatc
    13201 actttggctg ctttctccag cagcttaagc atgacgttaa cgccaacgct aaaattggcg
    13261 gcaaagacaa tcgcaatatc ggcggcggcg tcacgaatcg cctgtttacc ggcttcgtca
    13321 aaccccgtag tgccgatcac catccctttg ccatgctggc gacaaaaagc gagatggttc
    13381 agcgtacctt ccggacgggt aaaatcgata aacacatcaa aatcatcttt taccgcatcg
    13441 aggctgcttt gcacggtaac gcctgttttc cctgcaccag ccagctcacc ggcgtcgctg
    13501 cccagtaaag aagatccttc gcgctccagc gccgcgccca gctgcacgcc ttctaatgcc
    13561 agcgccgcct gaatcaactg gcggcccata cgccccccgg ctcccgcgat ggcaacgcgg
    13621 atgtttgcat catgcatagc tattctcttt tgttaatttg catagaccgt tttcagagta
    13681 accagcgcat cgctacgccg ccagccaaaa acgctgataa ttagaaatta atgataaaca
    13741 gggagggata tcagtgaaag gcatgagtcg atgaatgact gcatgccaat aacatgtgac
    13801 aggttacgac gccagagcca gcacctcagc cacccactgc tggaagcctt tcacatccag
    13861 atccaatgcc acctgtacat tggctggctt gcccaggcaa ccgtcgatat caaccaccgt
    13921 cgtgcctgag gtaaattcgc cctgagtttc cactgccaca aaacagggtt tgagagtgaa
    13981 caggtccggg cgcaccagcc aggcgatggc gcagagatcg tgcattcgca gaccgctttg
    14041 catactgccg ctacggtagt ggctaaacag tgcatgaagc atttttccgg tgcgattcaa
    14101 ctccggcaaa gtagcgagat agtcaggggt taatattgcc tgattggtga catccagacc
    14161 gcacatgacg atttcgattc cgctacggaa gacacaggca gccgcttctg gatcggcagc
    14221 gatattaaac tcggcgtttg gtgtgcagtt gccgcgacca gcagaaccgc ccatgatcac
    14281 cagacgacga atatacggtt tacattccgg gcattgtgaa agtaacagcg caatattggt
    14341 taacgggccg atggccacca gggtaacggg ctctggtgca cgcatcaggg catcccgaat
    14401 cgccagaaac gctggtatcc cgagcggctt tcggttgtgc tcaacaaagt cgtagccagc
    14461 cattcccgat tcgccgtgca cagatgccgc atcacgcggt gcgcgtacca gcggcacagc
    14521 ggctccctgg gcgagcggaa tctccgcatt ccagaaatgc agcagttgca gggcgttacg
    14581 ggtggttttc tcaaccgaga cattacccgc gacggtggtc atcagttgca ggtcgagttc
    14641 gggtgcaaaa atcgcggcgg caatggcgac ggcatcgtca atgccggggt cagtatcgag
    14701 gaagataggt aaacgcatgt tttctccata aaaaatgccg gtaacaagac cggcattttc
    14761 gcataactta ggctgctaat gacttaatcg acttcacgaa tatcgacacg cagctctttc
    14821 ggcacttcga aaacaatgtt ttcttcacgg ccttccagcg gaatggcttc accaccgccc
    14881 agctgctgca aacgtgccac cacattttgc accagaatat ccggagccga tgcgcccgca
    14941 gtcacgccga cgcatttaac ctctttcacc cactcttcct ggatatcttt cgcatcgtca
    15001 atcaaaaacg cgcgtttgcc catacgctgg gccagctccg ccagacggtt ggagttggag
    15061 gagtttttcg aaccgaccac caacacaact tccgcctgtt ctgccagggc gcgtaccgct
    15121 tcctgacggt tagtcgtggc gtagcagatg tcatctttgc gcggaccgac aattttcggg
    15181 aagcgtttac gcagcgcgtc gatcacatca gacgtgtcat ccaccgacag cgtggtctgg
    15241 gtcataaagg agagcttctc ttcgtttttg accgtcagtt tccacacatc gtccggcgat
    15301 tcgaccagat acattccccc ttccgggtta ctgtactggc ccattgtccc ttccacttcc
    15361 gggtgcccgg cgtgaccgat gagaatagat tcttcgccac ggcgactggc gcgggcgact
    15421 tccatatgca ctttggtcac cagcggacag gtcgcgtcaa ataccgtcaa atcgcgactt
    15481 tttgcttcgt tacgtaccgc ctgagaaaca ccgtgtgcgg agaaaatcag gatcgcgccg
    15541 tccggtactt cgctaatctg ctcaataaag atagccccac gctcacgcaa gctatcgacc
    15601 acatagcggt tatgtaccac ttcgtgacgg acatatatcg gtgcgccgta aatggccagc
    15661 gcgttttcaa caatgctgat agcgcggtct accccggcac aaaaaccacg cgggttggcc
    15721 aacaggatct gcatgttacg cctccagtgc cggatcgatt tccagcactt caatatcaaa
    15781 atgaacggtc tgcccggcca gcggatggtt gaaatcaacg gtaatggagt cgccgttaat
    15841 ttcgcggatc acgccaggca tctcactgcc atccattgcg gtaaaaagca tgattgcgcc
    15901 aatttctggc tcgcctgcat ccataaattc acggcgggag aagtactgaa tcaggtccgg
    15961 tgacggcacg ccaaacgccg catcgggctc cagcgagaag gtggttttat cgcccacttt
    16021 cagccccaac aggtgttgct ccagcccttc agaaagagaa gcatcgccca ggcggaacag
    16081 cgccggttta ccgttgttgc gggtagactc agcggtggtg ccatcatcga gtttcagcgt
    16141 gaagtgcacc aggacggcgc tattgctctg tacagattca gacatgcagg tttgctctta
    16201 tattttgagg atttttgccg gatgcggcct acgatttcgc agcaatctgt aggccttatc
    16261 cggcagggtt tattattgtt ttttcgcttt agaaggcaaa aaaccttcca gcacaatcag
    16321 tgccgcaccg acacaaatgg cagtatcggc aaggttgaag gtggcgaagt gccagtcgcc
    16381 gacgtagaag tcgatcatat cgacaacgaa gccgtgccac aggcggtcga acaggttgcc
    16441 cagcgcgccg ccaataatca gcgcataagc gatattgttt agcttctgcg tggccttcga
    16501 gcgatacatc atcactgcca ggatcacgct aataccaatc gcaataccgg caaagaacca
    16561 acgctgccag ccgccgctat cggcaaggaa actaaacgcc gcgccatagt tacgcgcata
    16621 atgcagatta agcgacggga acagcgggac cgtatccccc agagcaaagt tctggaggat
    16681 caggtatttg ctgcccagat cgataatcag cacgactacc accagccaca gccagcgtag
    16741 ccctgttgaa cagatcgatt gactcatcag gcaaacttac gtttttcacc gtcaccggcg
    16801 acgttgctga cacagcggcc gcagatttct gcgtgttccg ccaccttgcc gacatcctgg
    16861 gtgtagtgcc agcagcgtgg gcacttctca ccttcggctt tactcaacgc gactttcagc
    16921 cctttgagta cttcgctctg ctgagcatca gcaggtgcgt cgttatagtc tgcaacggta
    16981 gcgccggagg tcaacaggac aaatcgtaat tcatcgccca gcgcggtcag tttcgccgac
    17041 agttccggtt ctgcatacaa ggttactgcc gcttccagcg agccacccac tttcttgtcg
    17101 gcacgcgctt gctcaatgac tttgttcact tcgccacgca ctttcaacag ctcgtcccag
    17161 aacgcatcgt tcatcgcttc actgtctgcc aggccaaaca ggccttcgta ccactcaccg
    17221 gtgaagacgt atttttcacg ttcgcccggc aggtagcccc acacttcatc agcggtgaag
    17281 gagaggattg gtgccatcca gcgcaccagc gcttctgcga tgtgatacag cgcagtctgg
    17341 cagctacgac gcgccacact gtccgctttg gcggtgtact gacggtcttt gatgatgtcg
    17401 aggtagaagg aacccatctc aacggagcag aagcgcatca gacgctgtac cacttcgtgg
    17461 aaatcgtatg cttcgtacgc cttgaggatg tcttcctgtg ccgctttcgc acaacctacg
    17521 gcccagcgat ccagtaccac catctcttcc ggtttcacca tatcttttgc tggatcaaaa
    17581 ccgttcaggt ttgccagcag gaagcgcgcg gtgttacgga tacgacgata gctatcggca
    17641 gcacgtttca ggatctcgtc agaaacggcc atttcaccgg tgtagtcggt tgatgccacc
    17701 cacagacgca gaatatccgc gcccagtttg ttcatcacat cctgcggcga aacggtattg
    17761 ccgatggatt tagacatctt gcgaccctga ccatccacgg taaagccgtg ggtcagtacc
    17821 tgacgatacg gcgctttgcc tttcatcgcg gtggagatca ttagggaaga catgaaccaa
    17881 ccacggtgct ggtcagaacc ttccagatac atgtccgctg cgtgaccggc aaattccgga
    17941 cgcacgtcaa caacagaaga gtgggtagat ccggagtcaa accatacatc caatgtgtcc
    18001 ggcactttca cgtactgatc agcttcatcg ccgaggatct ctttcgcatc aagatcccac
    18061 cacgcctgga tgccgtcaac ttcaacgcgt tttgccactt cttccattag ttcgagagta
    18121 cgcggatgca gctcttccgt gtctttgtgc acgaacagtg acatcggtac gccccaggtg
    18181 cgctgacggg agatacacca gtcaggacgg ttagcaacca tcgactcgat acgcgcctgg
    18241 ccccagtccg ggatccactg cacgcctttg atctctttca gtgactgcgc acgcagacct
    18301 ttctgatcca tgctgacgaa ccactgcggc gtcgcgcgga agatgatcgg cgttttgtga
    18361 cgccagcagc acggatagct gtgctgcatt ttctcaacgt gcagcagcgc gcctttttcc
    18421 tgcagcagcg caacgacgat gtcgttcgct ttgaagacgt tcacgccatc cagcgtcgga
    18481 taagtgcccg gcagataagt gccgtccggg ccaaccgggt tagcggtttc caggccgtat
    18541 ttctgaccga tcacatagtc gtccgggccg tggccaggcg cggtgtgaac ggcaccggta
    18601 ccggcatcca gggtaacgtg atcgccgagg attgccggaa cgtcgaagcc cataaacgga
    18661 tgggtaaagc gcagcaactc aagctccgca ccttttaccg tgccgagaat ggtgtaatcg
    18721 gtcacgccga tacgctgcat tacgctttca accagatctt tcgccagaat cacggcctga
    18781 ccgtcgatct gcaccagcgc atagtcgaaa tcaggtgcaa tagagattgc gcggttcgca
    18841 ggcagagtcc acggcgtagt ggtccagatc accagcgaga ttgggccgtt aacgttgctt
    18901 acggcaaatt ttgctttcag tgcatcctga tcgaccgcct gaaaagcaac gtcgatggac
    18961 ggagaagttt tgtcgtaata ctcaacttcc gcttccgcca gcgcagaacg gcagtcaacg
    19021 caccagtgaa ctggcttcgc gcctttgtgc aggtgaccgt tgccgatgat tttgcccagc
    19081 gcgcggatga tgttggcttc agttttgaag tccatggtca ggtacgggtg cgaccagtcg
    19141 cccagcacgc ccagacggat aaagtctttg cgttgaccgt caacctgggt cgccgcgtat
    19201 tcgcggcact tggcgcggaa ctcggcggcg gtgaatttct cacccggctt accgtattct
    19261 tgctctactt tcagctcgat cggcagaccg tggcagtccc agccaggcac atacggcgag
    19321 tcataaccgg aaagcccttt ggacttcacg ataatgtctt tcagaatctt gttaaccgag
    19381 tgaccaatat gaatgctgcc attcgcataa ggagggccat catgcagaat gaaggttttt
    19441 ttgccttttt tagccgcacg gatgatgccg tacagatcat catcagtcca acgcgccagc
    19501 attcccggtt cgcgcttggc gagatcgcca cgcatcggga accctgtttc cggcaaattc
    19561 agggttgatt tatagtcact catcagattc tcggttccgt gtttcggttt gattacataa
    19621 caggcttaag ccggttttgt tagcccaaaa aattcgcggg cggttaattc atcacgcgca
    19681 atctgcgctt tcagttcgtc cagcgacgca aatcgctgct cattgcgtat ttttttacgc
    19741 agcactactt gtatatggcg accgtaaagg tccattgcaa catctaacaa atgcacttcc
    19801 agctgctggc gaataccggc aaccgttggg cgtgttccaa tgtttgccac gccgggtaac
    19861 ggcttttcac cgaggcccag cacttctacc gcataaaccc ctttcaccgg ggaaacctga
    19921 cggcgtagcg gtacattcgc cgtcgggaaa cctatagtgc gccctaattc atcaccgtgg
    19981 actacacgcc cggagatggc aaacgggtgc cccagtaaac tttctgccag agccagattg
    20041 tcatccgcca gcgcctgacg cacagcggtg ctgctgatac gcacgccacc ttcgcaaaaa
    20101 gtttgcgtac tggtgatatc gaagccgtat tccatgccag ctttctgtaa taacaagaaa
    20161 tcgccttcac gaccagcgcc aaagcggaaa tcatcaccta cggcaagaaa ttttacgcgc
    20221 aaatgcttca ccagaagatc gctgatgaaa ttttgcgcgg ttaacgccgc gaaacgcctg
    20281 tcgaaacgca cgcacagcac gtaatcaacg ccacactctg caaggtaacg cagtttttcc
    20341 cgcagccggg tcagccgtgc cggggcttta tcggtagcaa acagttccag tggttgaggt
    20401 tcaaaaagca tcaccatcac cggtaagttg cgcttgcgcc cttcttcctg caagccctgt
    20461 aacagcgcgc gatgaccgcg atgcacgccg tcgaaattac caatagtcag cacacaccct
    20521 tcttgcgggg cctggctgag attatgtatg ccgcgtatca gcttcatgtc tggctcaaaa
    20581 cagtgaaaat cgtccgagta taccttgtac agcggtcaag gttaaccggc gattgagtac
    20641 cgaaatcaga aagaagtggc ttttcattgc catggcgcaa atcacgggaa gaaagtgacc
    20701 gcctgctgca atttttatcg cggaaaagct gtattcacac cccgcaagct ggtagaatcc
    20761 tgcgccatca ctacgtaacg agtgccggca cattaacggc gcttatttgc acaaatccat
    20821 tgacaaaaga aggctaaaag ggcatattcc tcggcctttg aattgtccat atagaacaca
    20881 tttgggagtt ggaccttggc taatatcaaa tcagctaaga agcgcgccat tcagtctgaa
    20941 aaggctcgta agcacaacgc aagccgtcgc tctatgatgc gtactttcat caagaaagta
    21001 tacgcagcta tcgaagctgg cgacaaagct gctgcacaga aagcatttaa cgaaatgcaa
    21061 ccgatcgtgg accgtcaggc tgctaaaggt ctgatccaca aaaacaaagc tgcacgtcat
    21121 aaggctaacc tgactgcaca gatcaacaaa ctggcttaat cgccaatttg ctgaagcttt
    21181 gtgaaaaagc ccgcgcaagc gggttttttt atgcctgctg cttttatgat gcgtgagaga
    21241 acacactacc ggttgccaga tagtcaaaag atgctggctt ttgcagacat gccgttacac
    21301 tttctgaact cttcgtggct taagaatcgt agaaccatcg gctgatacat tctgaacaag
    21361 aatatctact caatctccag actcccctac aaagaatgtg gatattgtga tacacattga
    21421 ggtattaaac ttaagaccac tctatttttg aattaaattc ctatacactt taaccattca
    21481 taaatatctc cttattagat cttgtaaata attaacaaca atcaacacag gttaaatacc
    21541 tatttatatt ctacttcata a
//
LOCUS       DAZGSP010000086        21214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZGSP010000086 DAZGSP010000000
VERSION     DAZGSP010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.086"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            243..743
                     /gene="tssB"
                     /locus_tag="ACHK74_003584"
     CDS             243..743
                     /gene="tssB"
                     /locus_tag="ACHK74_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFP0222333.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            778..1002
                     /locus_tag="ACHK74_003585"
     CDS             778..1002
                     /locus_tag="ACHK74_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222334.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1053..2528
                     /gene="tssC"
                     /locus_tag="ACHK74_003586"
     CDS             1053..2528
                     /gene="tssC"
                     /locus_tag="ACHK74_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP0222335.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2535..2948
                     /gene="tssE"
                     /locus_tag="ACHK74_003587"
     CDS             2535..2948
                     /gene="tssE"
                     /locus_tag="ACHK74_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP0222336.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2952..4802
                     /gene="tssF"
                     /locus_tag="ACHK74_003588"
     CDS             2952..4802
                     /gene="tssF"
                     /locus_tag="ACHK74_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP0222337.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFTRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4766..5848
                     /gene="tssG"
                     /locus_tag="ACHK74_003589"
     CDS             4766..5848
                     /gene="tssG"
                     /locus_tag="ACHK74_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP0222338.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5873..7153
                     /gene="tagH"
                     /locus_tag="ACHK74_003590"
     CDS             5873..7153
                     /gene="tagH"
                     /locus_tag="ACHK74_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFP0222339.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGTPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7150..7674
                     /gene="tssJ"
                     /locus_tag="ACHK74_003591"
     CDS             7150..7674
                     /gene="tssJ"
                     /locus_tag="ACHK74_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFP0222340.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7677..9008
                     /gene="tssK"
                     /locus_tag="ACHK74_003592"
     CDS             7677..9008
                     /gene="tssK"
                     /locus_tag="ACHK74_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP0222341.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQHFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDTRTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9013..9774
                     /gene="icmH"
                     /locus_tag="ACHK74_003593"
     CDS             9013..9774
                     /gene="icmH"
                     /locus_tag="ACHK74_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFP0222342.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9783..12596
                     /gene="tssH"
                     /locus_tag="ACHK74_003594"
                     /gene_synonym="clpV"
     CDS             9783..12596
                     /gene="tssH"
                     /locus_tag="ACHK74_003594"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFP0222343.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWQKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGHVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESESESESESESEQDNTGAEPADEAGSEQPEETAE
                     TVSPVQRLAHLTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVIT
                     DLPKWLGDTIKGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQL
                     AELLYGGRQYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLL
                     DEVEKAHPDVLNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPET
                     MQEVLYPVLADFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVI
                     IEPEVTDEIMSRVTRAENGARMLESVIDGNMLPPLSLLLLQKMAANTAVARIRLSAVD
                     GAFTADVEDAQNDESVTKDETVL"
     gene            12593..13336
                     /gene="vasI"
                     /locus_tag="ACHK74_003595"
     CDS             12593..13336
                     /gene="vasI"
                     /locus_tag="ACHK74_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFP0222344.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKHRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13341..14753
                     /gene="tssA"
                     /locus_tag="ACHK74_003596"
     CDS             13341..14753
                     /gene="tssA"
                     /locus_tag="ACHK74_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFP0222345.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14862..18296
                     /gene="tssM"
                     /locus_tag="ACHK74_003597"
     CDS             14862..18296
                     /gene="tssM"
                     /locus_tag="ACHK74_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFP0222346.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALMWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QALRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKELNAAPPESEEKLAVLRVMRMLEDKSGRNNQ
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGHFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18307..19756
                     /locus_tag="ACHK74_003598"
                     /pseudo
     CDS             18307..19756
                     /locus_tag="ACHK74_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19683..20165
                     /locus_tag="ACHK74_003599"
     CDS             19683..20165
                     /locus_tag="ACHK74_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP0222347.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            20209..21123
                     /locus_tag="ACHK74_003600"
     CDS             20209..21123
                     /locus_tag="ACHK74_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222348.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
     gene            21133..>21214
                     /locus_tag="ACHK74_003601"
     CDS             21133..>21214
                     /locus_tag="ACHK74_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222349.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNAS"
BASE COUNT         5077 a         5718 c         5794 g         4625 t
ORIGIN      
        1 ttgccataat acacctgacg cgaattgcac aggttcatac aatctattta acgcttgcag
       61 aatacccctc tttacgatcg ataaaaagtc cttttcttat ttattttgaa gcgtaaaatg
      121 cggggtgatg aaggagtggt gattttattt tcaccgccat caaggcggca tttccgatat
      181 tttttgcggt acgaatatgc cgtgttcatg gcctgtacga gataacatga cggatcactt
      241 ttatgagcaa aaaatttgaa ggcagcgtcg cgcctcgcga gcgtattaat atatcgtatg
      301 tcccaaaaac agatggccag acggctgaag tggaacttcc gcttaacatg ctcgttgtcg
      361 gcgataccgg aaatacgcag gaaacatcct cactggatga acgtcaggca gtttccgtta
      421 acaagcacaa tttcggtgca gtgatggctg aagcagccat tggcctcaat tttactgtcc
      481 ccgccaccct gaagggaagc acaacagacg atgaactgaa tgtggcgctg aatatcaaat
      541 cactggatga tttttcacca gatagcgtgg cccggcaggt tccggaagtg aataaattgc
      601 tggaactgcg tgaagcactg actgcactga aggggccgat gggtaacctt ccggcattcc
      661 gtacccagct ccaggcattg ctggaaaatg aagagtcccg cgagcagttg ctcaaagaaa
      721 tcggtctggt gagcaataaa taaccaccac tatttcatta aacagggtta tccaggcatg
      781 acggcacaca aaaatatctc cgggacatac cacctctctg ttgcagacat tctgcaggtg
      841 gtttaccagg tatgtttcag cccgtccgtt gaaatcaacc aggatggtgt ggctgcgctg
      901 attacaacac tggatcgccg tatcagtgat ctattggatg agattatcca tttttgtgag
      961 tttcagcaga gcgcgtcgca ctggcagcgc gtacttcact gacggcgaaa agccggtttc
     1021 agaaacacag ctaaaacaga aggattttca gtatgtcttt acaggaagag gaactcgtat
     1081 ccagtcatgc cgggcagcct gagcaggcat cgtcgctgct tgatcaaatc atggcacaga
     1141 cccgtattca gcccggttcg gaaggttatg acgttgcccg ccagggtgtg acagccttta
     1201 ttgccagcat cctgcaatcc actgcatctg ccgaaccggt caataaactg gccgtggaca
     1261 gcatgattgc agatattgat gaacgcatca gccgccagat ggatgtcatc atccatgcgc
     1321 ctgcctttca gcaggtcgag tcgttctggc gctcactgaa aaccatggtg gatcgcgttg
     1381 atttccgtga aaacatcaag gtcaacgtgc ttcatgtcac caagcaggaa cttctggaag
     1441 actttgagtt tgcgccagaa attattcagt ccggatttta taagcacgtt tattcgtccg
     1501 gtttcggaca gtttggcggt gaacccatcg ctgccgtgct gggcgcttat gagttcaaaa
     1561 ataccgcgcc tgacatgaaa ctgctgcaat acgtcagtgc cgtgggcgca atggcgcacg
     1621 caccgttcct gtcctccgtt tccccggagt ttatggggct gaactcgtgg accgaactgc
     1681 ctaatatcaa agatctttat gccatctttg aaggtccggc ctacaccaaa tggcgcgccc
     1741 tgcgtgactc ggaagattcg cgctatttag ggcttacggc accccgcttc ctgcttcgcc
     1801 agccctattc accaacagat aaccctgtta agaactttaa ctattacgaa gatgtcagcc
     1861 agaaccacga agattatctg tggggtaata cggcctggat gctggcatgc aatatcgcag
     1921 acagttttgc caaataccgc tggtgtccaa acattatcgg tccgcaaagc ggcggcgcag
     1981 tgaaagatct gccggtgcat ctgttcgaaa cgatggggca gattcaggcc aaaatcccaa
     2041 ccgaggtgct ggtcaccgat cgccgcgaat ttgaactggc tgaagagggt tttatcaccc
     2101 ttaccatgcg taaagactct gataatgcag cctttttctc tgcaaactcg gtacaaaaac
     2161 cgaagcactt tccgggaaaa gatgcagaaa ccaactataa gctgggtacg cagcttcctt
     2221 atctcttcat catcaatcgg ttagcgcact acatcaaggt gttgcagcgt gaacaactgg
     2281 ggtcatggaa agaaaggagc gacttagaaa gagagcttaa tacctggatc cgtcagtatg
     2341 ttgccgacca ggaaaacccg cctgcagacg tgcgcagccg caaacctctg cgcgctgcca
     2401 aagttgaggt tatggatgta gaaggcgaac ccggctggta ccaggttgcg ctaagcgtga
     2461 ggcctcattt taaatttatg ggggcaaatt ttgagctttc cctggttggc cggttagaca
     2521 gggagtaaca caccatgata ccggatcggg aaagaaccac aggttctctt tttgagcgta
     2581 tggaagcctc ttcggccaga aaccggcagg gaggcagtat tcattcgctc aggcaatcca
     2641 ttcgccagaa cctacgaaac attcttaata cccggtccgg tagttgccgg ggtgcgcctg
     2701 agctgggcat tgatgaaccg gaaggggctg aaaacttccg cgaatccatg agtcgggcca
     2761 tcgagcaatg tatagaacgc tatgaacccc ggatctcaca cgctgaagtg caggcagtgg
     2821 tttcatccgc ctccagcccg ctggatatga cgtttcatat tacagcctgg gtgacattta
     2881 acgaaacgca tgaggtgctt gaatttgata tggcaccgaa tggcagccag cattaccggg
     2941 tggactgaca aatggaattt gaagaacgct atttccggga agaactcgat tacctgcgcc
     3001 agcttagcaa gctgctggca acggaaaaac cccatctggc ccgcttcctg gccgaaaaag
     3061 atgcggatcc ggatattgaa cgcctgctgg aaggggtggc ttttcttacc ggcaatctcc
     3121 gccagaaaat tgaggatgaa ttcccagaac tgacgcacgg gcttattaag atgctatggc
     3181 ctaattacct gcgtccggtt ccggcaatga cccttattga atatacgccg gatatggata
     3241 agtcttctgt accggtgtta atcccccgta atgagcagtt tacaaccaac gccggggaaa
     3301 tcagagttga tgaagtgctg ccctctgatg ctaaaaagga ggagccgcct ccctgtacct
     3361 tcactctctg ccgggatatc tggctgctgc ccgttcgcct ggagcagatt gaaaaccgca
     3421 gtacgacccg taatggtgtt atcaacatca ccttttcggt cgcaccggga acggacttcc
     3481 gcacgctgga tctgaacaaa cttcgcttct ggctcggcaa tgacgacaac tatacccgtg
     3541 accagcttta tttatggttc tgcgaatact tgcagggtgc cgacctgact gtgggtgaac
     3601 agcatattcg cctgcctgag tttatgctaa aagctgtcgg ttttgagccg caggatgcca
     3661 tgctgccctg gccgaaaaac gtccacagcg gctaccggat ccttcaggag tatttctgtt
     3721 accccgatgc gtttctcttt tttgatcttt gtggttgtcc ggctttgcct gacggattgc
     3781 aggcggaatt ctttaccctg caactgcgtt ttacgcgccc tttgcccgtg gacatccggc
     3841 tgcgccgcga ttccctgcgc ctgtattgcg cacctgccat taatttattt atccaccatg
     3901 cagaagccat cacgctggac aaccggcggg cagactatcc gctggttccc agccgccatt
     3961 acccacaaca ttacgatgta ttttccgtta acagtgtggt gagccaggtc caggatatgt
     4021 tcaggaaaaa agatctgggg cgtcctgttt cgacgcaggc cgcgcgccag tggccagcct
     4081 ttgaaagttt cagccatcag atggaataca gccggaagcg ggaagtggtg tactggcatc
     4141 accggaccaa aacatccctg ttccatcgcg gctttgatca tacccttgcc tttatacatg
     4201 ctgatggcag ttatccgtca gacgaatctc tgctcagtaa tgaagtggtt tcggtatcgc
     4261 tgacctgtac caaccgtgag cttccgtcac aaattcgttc cggcgatatc accggcacaa
     4321 ccggtaaaaa tgcggctgtt gcttcatttc gcaacattac ccgcccgacg caaccactct
     4381 ggccggtcat tgatggcagc ctgcactggt ccctactctc cgccatgaac ctgaattatc
     4441 tgtcattact ggatacggac gcgctgaagc aggtcatcgc caactttgat cgccacgcaa
     4501 tccatcatcc gcagacggca cggctgtcac aacaaaagct ggatgccatt gagcgtctgg
     4561 agacccgccc cgttgatcgc ctgtttacgg gtattcccgt ccggggactg gcctccacgc
     4621 tgtatctgca cccggagccg tttgtctgtg aaggggaaat gtatctgctc ggtacggtgc
     4681 tttcgcattt tctgtcgctg tacgccagcg ttaactcatt ccacatgctg accgttgtga
     4741 acacagaaag ccaggagaca tggaaatgga cggaaagaat cgggcagcat cctcttatct
     4801 gagtccggga aatcccccgg cagataaaga gcagaatgat ccgctggcac aggttttcca
     4861 taatgcctgc tcttacaatt tttttgcgat ggcggagctg ttgcaccgcc tggcaaaggg
     4921 tgaaaagggg acgccagaat tatccctgcg tgacgatccg gcacaggaaa ccctgcgttt
     4981 cagcgccgat gccagccttg ccttcccgtg cagtgatatc agtgcactga aacgggatac
     5041 gtcaggggca ttcaggatga ccaccacctt tatggggtta caggggagcc agtcgcctct
     5101 ccccggttat tacctcgatc acctggcctg gaaagcggta catgaacaaa gtccggtagg
     5161 tgattttctg gatatgttca gccaccgcct gacccagttt gtctggcata tctggcgtaa
     5221 gtaccgctat cacatcagtt ttcgtaacgg cggtgtggat gcgttctcgc agcgcatgta
     5281 ctcccttgtc gggctgggcc accgccagct tcgcgataag ctggcgatta atcacagcaa
     5341 aatgctggcg tattccggga tcctggcaaa tccgggacga tccccggaaa taatctgtgg
     5401 cctggtgtcc cactgctttg atttgtcaga agtgacgctg caaaactggc aacgtcgcaa
     5461 ggtggatatt gaaccggatc agcaaaatag tctggggagc tattctctta aaaatggcga
     5521 aaaactggcg ggaagatcgg tactgggcaa ctttgttctg ggtacacgag tgcccgatct
     5581 cagcggaaaa ttccagctca gtattaccag cctgacccga aaacagttcc tctcttttct
     5641 gccgtccggc gaaaacttcc tgccactgac gatgttcgtg tccttcatcc tgcgcgatca
     5701 gcttgcctgg gacttacatc tggggctggc gccggagcag gtgggcgcaa tgcgcctggg
     5761 cgataacaaa agtgcgctgc tgggctggac cagcttcctc ggcacaccgg aagaacgacc
     5821 ctcagtcacc atcagggtac ggtcgtaaat tatgataatc acggagcaga atatgccgga
     5881 agagaaacta cagacgcttt cattgcaggt cattaacggc agtgagctgg aaagcggacg
     5941 ggcggcgcgc tgtctgttca cacagcaggg aaatgtcggc catggccccg aatgccactg
     6001 gtcggtacag gatcgtcagc agagcattcc ggcccaggct tttaccgtta tcctgcacga
     6061 tggcacattt tgtctgcgcc cgcagacggc acaactgtgg ctgaatcagg caaaagtcac
     6121 agcaacatca gacctaatac agttgcgcca gggcgatgag atccagatcg gacggctgat
     6181 ggtgagggtt catctgaacc ggggagatat tccccattac gatgaggaaa tggccactcc
     6241 cgaaaccatc gttaccaatc gcgatatgct cacggatacc ctgctatcaa cggagggtac
     6301 gccacactat ccgggaatga ctcaccggca ccaacttgca gacaccgtgg taaacggttt
     6361 ttctgccgat ccactccagg cacttcagtc cgaaagcctg attaccacgg gcgatccgct
     6421 ttcaggcatt gcggctgtcc ggccatcggc accgctgtcc gatccggcaa gtaatggggg
     6481 gatcaatact ccgtttatgg atctgccgcc catttatgcc agccccggcg atcgtaatga
     6541 cgacgtctct gcggcagaaa tggcgcaacg ccaccttgcg gtcaccccct tactgcgcgg
     6601 tcttggcggc tcgcttacca tgagcaattc cgacgatgcg gatgattttc tggaggaggc
     6661 cggacgaacg ttacaggccg caataaaagg tctgctcgat ttgcagcagc agcgtaacag
     6721 cctctcagac aaacatttgc gcccgctgga agataacccg ctgcgcctga acatggatta
     6781 cgccaccgcg ctcgacgtaa tgtttgccga aggtaaaagc ccggtacatc tggcggctcc
     6841 cgccgccgtc agtgaaagcc tgcgcaatgt ccgccaccac gaagaagcta accgggcagc
     6901 gattgtggag tcgcttcgtg tcctgctgga tgctttctca ccacaaaatc tgctgcgccg
     6961 ctttgtgcag taccgccgca gccatgaact gcgccagccg ctggatgatg ccggagcatg
     7021 gcaaatgtac agccattatt acgaagaact ggcctccgat cgccagcagg ggtttgagat
     7081 gctgtttaac gaggtctacg cccaggtcta tgaccgggtg cttcgtgaaa aacagcggga
     7141 gccggaagca tgaacaggaa ggcgtttctg gcctgcgtgc tgatgtgcgt catattaacc
     7201 ggctgcgaaa cggcgaaaaa aatcagtgag gtgatcaaaa acccggatat ccaggtcggg
     7261 agcctgaagg agcaaccttc ggaaataacc gtcaccctgc tgaccgaacc ggacaccaat
     7321 accaatgcgg aaggtgaatc agccgccgtg gatgtgcaac tggtgtacct gactgacgac
     7381 tcaaaattgc tggcagccga ctatgaccag atagccagta cgccgctacc ggatgtgctg
     7441 ggtaaaaact atatcgacca ccaggatttc aacctgttgc cggacaccat caaaaccctg
     7501 ccgccggtaa agctggatga gaaaacgcag tttattggtg tcgtggccta tttctccgat
     7561 gaccaggcca ctgaatggaa acaaatcgag acggtggaag gaaccggcca ccattaccgc
     7621 ctgctggtgc atgtacgtca aagcagcatc gaaatgaaaa aagaggatga ataattatgg
     7681 caaccacccg caacaaggtg atgtggcagg aagggatgct gatgcgccca caccatttcc
     7741 agcaacagca gcgttacaac gactacctgg ataaccagcg tttccgggcc atgaatgatt
     7801 tatcctgggg atttaccgaa ctcaccctca acaatgaact gctggcgcag ggtaagatca
     7861 tgattgacag cgcgtcaggc acactgcccg acggcaccgt cttttctatc cccgaccagg
     7921 acgcactgcc cgatccgctg cacccgcaac attttccgga cgagagaagc cgcaatatct
     7981 acctcgctct gccggtcgcc agtgatgtga gaaatgaaat cagcgacggg cggcgaatcg
     8041 ggcgttaccg gctgaattat gccgatgtcc gggatttgca ttcagaagaa ggtgacacgc
     8101 gaacactgac gctgggacaa ctgacgccgc gcattatgag cggtgcagaa gatatgagcg
     8161 cctatattac gctcccgctt tgccgtatca gtgatcgcca tgccgacggc tccctgacgc
     8221 tggatgacga ttttatcccc tcctgccaga atattcaggt cagtaagaaa ctgcgtgttt
     8281 atctcaaaga ggtacagggg gccattggcg gacgggcaag cgatctggca aaccgcattg
     8341 gctctccggc gcagagcggc atcgcggatg tggcggaatt tatgatgttg cagttactta
     8401 accgtaacca gacccggttt acccatcgcg ctcgtcgatc ccagctccac ccggaagatt
     8461 tctaccttga tcttgccggg ttgctgggtg aactgatgac ctttaccgag ccgtcgcgcc
     8521 tgccctgccc gcttgatgtg tatgatcatc atgacctgac caaaacattt aaaacgctgt
     8581 taccggaagt caaacgggcg ctgcataccg tactgtcgcc aagagccgtt aatctgccgc
     8641 tgcatctgcg tgacggtatc tggcaggccg atatccatga cacagaactg ctgcaatctg
     8701 ccacctttgt gctggctgtg gcggcaaaca tgccggtcga tcagatccag cgccagttta
     8761 tccagcagtc gaaaatttcc tcgccggaaa aaatccgcaa tatggtcagt gtgcagatac
     8821 ccggcattcc attgcgtgcc ctgatggtgg ctccccgcca gcttccttac cattccgggt
     8881 tcagctattt cgaactcgac aagagcggac aggcctggac agaaatggct gccgccgggg
     8941 ccgttgcact gcatgtatcc ggcagtttcc cggatctgaa catgcaactg tgggcgataa
     9001 gagggtaaga cgatggatga ggggtcactg tctctgccac catttaccgg atacgacgaa
     9061 aaaagccagc gcgactatca tctcgcgctt cgtggcaaca gcctgaatcc gatgatcgac
     9121 gcggccacgc cgctactcgg tatggtaatg cgcctttcaa ccatgaacag ccagacgatg
     9181 ccggagcatc tttttgctca ggtggtgacg gatgttcagg ccgtggagca actgcttcag
     9241 gagcagggct acgaaccggg cgtgattatc tcgtttcgct acatcctctg cacgtttatt
     9301 gatgaagcgg cgctcggtaa cggctggtcg aacaagaacg agtggatcaa gcagtcattg
     9361 ctggtccatt tccacaacga agcctggggt ggagagaaag ttttcatcct gctggagcgc
     9421 ctgatccgtg aacccaaacg ttaccaggat ctgctggaat ttctctggct ctgtttttct
     9481 ctgggatttc gcgggcgcta caaagtggca gcacaggatc agggggaatt tgagcagatc
     9541 taccgccgcc tgtaccacgt cctgcataaa ctacgcggcg atgccccttt cccgctgtta
     9601 caccaggaca aaaaaaccca gggcgggcgt tatcagctca tcagtcgcct gaccattaaa
     9661 catatttttt gtggtggcgt tgtcgtgctg gcgttgtttt acctgttcta tctgttgcgg
     9721 ctggacagcc agacgcagga catcctgcac cagctaaaca aattacttcg ataaggacgt
     9781 ttatgatcca gattgatctt cccacgctgg taaaacggct taacctgttc tcccgccagg
     9841 cgctggagat ggccgcctct gaatgtatga gccagcaggc agcggaaatt accgtcagcc
     9901 atgtgcttat tcagatgctc gccatgccac gcagtgacct gcgggttatt acccgccagg
     9961 gcgatattgg catggaagag ttgcgccagg cgctgacggt ggagaactac acaactgccc
    10021 gttctgcgga cagctacccg gcattttccc cgatgctggt tgagtggcag aaagagggct
    10081 ggctgctggc gtcggctgag atgcagcaca gcgaattgcg tggcggcgtg ttgctgctgg
    10141 ccctgctgca ttcgccgctg cgttatatac cgcctgctgc cgcccggctg ttgaccggca
    10201 ttaaccgtga ccgtctgcaa caggactttg tgcaatggac acaggagtcg gcggaatcag
    10261 tcgtgccgga tgcagacggt aaaggcgcag gcacactgac ggacgctgct gacaccctgc
    10321 ttgcccgcta cgccaaaaac atgaccgcag acgcccgtaa cggcaggctt gacccggtac
    10381 tgtgccgcga ccacgaaatc gacctgatga tcgacattct ctgccgccgc cgcaagaaca
    10441 acccggtggt ggtgggcgaa gcaggcgtgg gtaaaagcgc cttaatcgaa gggctggcgc
    10501 tgcgcatcgt ggcaggccat gtgccggaca agctgaaaaa caccgatatc atgacccttg
    10561 acttgggcgc attgcaggcc ggagcgtcgg tgaagggtga attcgaaaaa cgtttcaaag
    10621 ggctgatggc ggaggtcatt tcctccccgg tgccggtcat tctgtttatc gacgaagcac
    10681 ataccctgat tggcgcgggc aaccagcagg gcgggctgga tatctccaac ctgctcaaac
    10741 cggcgctggc gcgcggcgag ctgaaaacca tcgccgccac cacctggagc gagtacaaaa
    10801 aatacttcga aaaagatgcc gccctgtcgc gccgcttcca gttggtgaag gtcagcgaac
    10861 ccaacgctgc cgaagccacc attattctgc gcggtctgtc ggcggtctat gaacagtctc
    10921 acggcgtgct gattgatgat gacgccttgc aggccgctgc gacattaagc gagcgttatc
    10981 tctccgggcg tcagttaccg gacaaagcga ttgatgtgct ggataccgcc tgcgcccgtg
    11041 tggccatcaa cctgtcgtcg ccgccgaagc aaatctcggc gctgaccact ctgagccacc
    11101 agcaggaggc ggaaattcgc cagcttgagc gcgagcttcg catcggactg cgtaccgaca
    11161 catcacggat gaccgaggtg ctggtgcagt atgatgaaac gctgacggcg ctggatgaac
    11221 tggaagcggc ctggcaccag cagcagacgc tggtccggga gattattgcg ctgcgccagc
    11281 agttactggg cgtggcagag gacgatgcgg cgccgttgcc ggacgcagat accgtggagg
    11341 atacgcagcc agagtcagag tcagagtcag agtcagagtc agagtcagag tcagagtcag
    11401 agtcagagtc agagcaggat aataccggtg ccgaaccggc tgatgaagct ggcagcgaac
    11461 agccggaaga gaccgctgaa acagtttccc cggtacaacg tctggcacat ctcactgccg
    11521 aactggacgc cctgcataac gaccggttgc tggtttcccc gcacgtcgat aaaaaacaga
    11581 ttgcggcggt gattgccgaa tggaccggcg tgccgcttaa ccgcctgtcg cagaatgaaa
    11641 tgtcggtcat caccgacctg ccaaaatggc tcggcgacac catcaaaggc caggacctgg
    11701 cgattgccag cctgcacaaa cacctgctga ccgcacgcgc cgacctgcgc cgtccgggac
    11761 gcccgctggg cgcgttcctg ctggctggcc ccagcggcgt gggtaaaact gaaaccgtcc
    11821 tgcaactggc agagctgctc tacggcggtc gccagtacct gaccaccatc aatatgtccg
    11881 agttccagga gaaacatacc gtctcgcgac tgattggttc gcctccgggc tacgttggct
    11941 acggtgaagg cggcgtactg accgaagcga ttcgccagaa accctactcg gtagtactgc
    12001 tcgatgaagt ggaaaaagcg cacccggatg tgctcaacct gttctaccag gcgttcgata
    12061 agggcgaaat ggcagacggt gaaggccgcc tgattgactg caaaaatatc gtcttcttcc
    12121 tgacgtccaa cctcggctac caggtaatag tcgagcatgc cgatgacccg gaaaccatgc
    12181 aggaagtact gtatccggtg ctggccgact tcttcaaacc tgccctgctg gcgcgtatgg
    12241 aagtggtgcc gtacctgccg ctgtcgaaag agacgctcgc caccatcatt gccgggaaac
    12301 tggcccgcct ggataacgtg ctgcgcagcc gctttggcgc ggaggtgatt atagaaccgg
    12361 aagtgacgga cgaaatcatg agccgcgtca cccgcgcgga aaacggcgcg aggatgctgg
    12421 aatcggtcat cgacggcaat atgctgccgc cgctctccct gctgctgttg cagaaaatgg
    12481 cggcgaatac ggcggttgcc cggattcggt tgtcggcagt ggacggcgca tttacggcag
    12541 acgtggaaga tgctcagaac gacgagtccg tcacaaagga tgaaacggtt ttatgagcag
    12601 gttgcgcgtt ttccggcaga caggatggtt tatcgcgggc ctgatgacgg gcttacccgc
    12661 cactgccgcg cccgcagaag catcgtcaat ggccggtgtg gcggtcgccg tcgccaccac
    12721 gaccccgccg gatgcgacag ccacgttaca ggccatgcag tcctgtcggc gggagtccgc
    12781 ggcgctggag cggcttgact gctatgaccg cctgctggca ccgctgtccc cgtcagggtt
    12841 tgacggggcg ctggtcaaag ccggttttgt gggcgaagcc tggacccgcg ccacggaaca
    12901 ggaaaaacac cgccagggca acaccacaga gctgctggtg acgcaggtgc cgggggagcg
    12961 cccgacggtg gtgataacca ccccggctat cggtcatgtt ccgccacgcc cggtgctgat
    13021 gttcagttgc gtggacaata tcacccggat gcaggtggcg ctgatgcacc ctctggatgt
    13081 ccatgatatc gccgtcactc tgaatgccga caaccgggca ttgcgcagcc actggtttgt
    13141 gcgcgagaac ggcaccctgc tggagtccag ccgggggtta tccgggattg atgaaattaa
    13201 gcaactgttc ggcgcaaaaa cgctgacggt ggataccggc gcagacaatg ccgccggaaa
    13261 gctgaccttt aacattgacg ggctggcaag ggcgattgct cccctgcgcg atgcctgcca
    13321 ctgggcggga gaataaaaat atggcaatgg acttacgcga tccgaatgtc tggatatcgc
    13381 acctgctgga aaacctgccg gaagagaaac tggcatcggc gctgaaagat gacaacccga
    13441 actgggagta tatcgacggc gaaatcgtca agctgggatc gctggcccac agtcaactgg
    13501 atatcccgga actccagcgc cgggggctgg tcattctggc ttcggagagt aaggattttc
    13561 gtctgctggc gcatctgctg agaaccctgc aacacgccgg tgatcctcat ctcgcattgc
    13621 gcctgctggc gctgtatgtg gagcattact gggcggtggc tgcgccgcag aatatggcgc
    13681 ataaaaaacg ctttgccagc caggtcatta aacgctttga aacaggtatt gaagtttttt
    13741 cacagaacgc cgccacagcg cagcgcgatg cgctgtcagg ggaactggca aaactggcgc
    13801 aatgctggca gtcacataac gccccggaac tggcacaggc tactgatgat ctttttgccc
    13861 tgtaccagcg tgcgtttaat cgtgcggcac cggctcctgt ccccactccg gcggcctccg
    13921 gtagttcacc acagaccacc gccacgtctg aaagcggcgt gacgcaaccc agtgctccgg
    13981 ctccccaaat cgtcatcgac agtcacgacg acaaagcctg gcgcgacacg ctgttaaaag
    14041 tggcggccat tttatgtgaa cgccagccgg attcaccgca gggctaccgt ctgcgccgcc
    14101 atgcgctttg gcagaacatc accagtacgc cccaggcgga aagcgatggc cgtactccac
    14161 tggctgcggt ctccgccgat atggtggcag attaccatgc tcagctcggt agtgccgata
    14221 tggcgctgtg gcagcaggtg gagaaaagcg tactgctggc tccttactgg ctggatggtc
    14281 actgcctttc tgcacagacg gcgctgcgtc tgggttacaa acaggtggca gacgccatcc
    14341 gcgatgaggt catccgcttc cttgagcgtc tgccccagct taccgggctg ctgtttaatg
    14401 accacacacc gtttatcagt gagcagacga aacaatggct ggcagcttcg cccgacgcca
    14461 aagttgcacc ggttgcgcaa atcggtgagg aatcgaaggc agccagagcc tgttttgctg
    14521 agcagggtct ggaggcggcg ctgcgatatc tggacatgct acccgaaggc gatccccgcg
    14581 atcagtttca ccgccagtac cttgccgcac agttgacgga agaggcgggg ctggtacagc
    14641 ttgcgcagca gcagtaccgg atgttgttca ggatgggatt gcagatgatg gtggcagact
    14701 gggagccgtc cctgcttgag cagctagaac aaaaattcac ggcagaacaa taaaagagaa
    14761 caggagtttt tgtgttcaga ttaccaacac cccgactact cagcggactc aaatcagccc
    14821 tgcgaccggc gatgccccgg tttaaagtct ctgctttctg gctgctgata ctggcgtgga
    14881 tttttctgct ggtgtggatc tggtggaaag gcccgacgtg gacgctgtat gaagaacagt
    14941 ggctcaaacc actggcgaac cgctggctgg caacggcggc gtgggggatt attgccctga
    15001 tgtggcttac cgtccgggtg atgaagcgcc tgcaacagct tgaaaaaatg caaaagcaac
    15061 agcgcgagga agccgttgat ccgctcagcg tggaactgaa cgcccagcag cgttatcttg
    15121 accgctggct gctgcgcctg caacgccatc tcgacaaccg ccgtttcctg tggcagttgc
    15181 cgtggtatat ggtcatcggc ccggcgggca gtggcaaaac gacactgctg cgcgaggggt
    15241 ttccgtccga cattatttat gccccggagg gcgcacgcgg cgcagaacaa cgcctgtacc
    15301 tcacgcccca tgtcggtaaa caggcggtga tctttgatat cgacggcaca ctctgcgctc
    15361 ccgctgatgc ggatatcctg catcgccgtc tgtgggaaca tgccctcggc tggctgaaag
    15421 aaaagcgcgc gcgccagccg ctgaatggga ttattctgac actcgattta cccgatctgc
    15481 ttaccgcaga caaacgccgc cgcgagcatc tgttacaggc gctgcgcagc cgcttgcagg
    15541 atatacgcca gcatcttcac tgccagttac cggtttacgt ggtacttacc cggcttgatt
    15601 tattgcaggg ttttgccgcc ctgtttcagt ccctgaacag acaggatcgc gatgcgattc
    15661 tgggcgtcac gttcacccgc cgtgcccatg aaaatgatga ctggcgaaca gagttgaatg
    15721 ctttctggca gacatgggtg gatcgaatga atctggcgtt gccggatctg atggtcgctc
    15781 agactcacac ccgcgcgtct ttattcagtt tttcccgcca gatgcaggga agccgtgaac
    15841 cgctggtgtc actgcttgag ggtctgcttg atggcgaaaa tatgaacgtg atgctgcgtg
    15901 gtgtctatct cacctcttcg cttcagcgtg gacagatgga tgatatattc acccagtctg
    15961 ccgcccgcca gtaccggctg ggcaataacc cactggcgtc ctggcccctg gtggacaccg
    16021 cgccttattt cacccgcagc ctgttcccgc aggcattact cgcagagcct aatctggcaa
    16081 cagagagccg cgcctggctg atacgttccc gtcgccgcct gacggttttc tctgccacag
    16141 gcggcgtggc agcactgctg ctcatcaccg gctggcatca ctattacaac ggtaactatc
    16201 agtctggcat caccgtgctt aagcaggcca aagcctttat ggacgtgccg cctccgcagg
    16261 gggaagatga ctttggcaac ctgcaactgc cgctcctgaa cccggtacgc gatgccacac
    16321 tggcctatgg cgactggggc gaccgcagcc gtctggccga tatgggactg taccagggac
    16381 gacgtatcgg gccttatgtg gaacagacct atctgcaact gctggagcaa cgttacctgc
    16441 cctcgctgtt taacgggctg gtcaaagagc tgaacgccgc gccgccggag agtgaagaaa
    16501 aactcgcggt gctgcgcgtg atgcgaatgc tggaggacaa aagcggacgt aacaatcagg
    16561 tggtgaagca gtatatggca aaacgctgga gcgaaaagtt ccacggtcag cgcgatatcc
    16621 aggcacaact gatgtcccat cttgactacg cgctggctca tactgactgg cacgcagagc
    16681 gtcaggcggg cgacggtgac gccatcagcc gctggacgcc atatgacaag cccgtggtat
    16741 cagcacagaa agaactgagc aaactgcctg tctaccagcg ggtttaccag agcctgaaaa
    16801 cgcgggcgct gggcgttctt cctgccgacc tcaatctgcg tgaccaggta gggccaacct
    16861 ttgaccaggt gtttacatct gccgatgaca acaaactggt tgttccacag tttcttaccc
    16921 gttacggcct gcaaagctat tttgtaaaac agcgcgatga actggttgaa ctgacggcga
    16981 tggattcctg ggtacttaac cttacccgca gcgtgaaata cagtgacgcc gaccgcgcgg
    17041 aaatccagcg ccagttgacc gagcagtata tcagcgacta caccgccacc tggcgggccg
    17101 ggatggacaa tctgaatatc cgcaattttg agtccatcgg acaactgacc ggggcgctgg
    17161 agcaggttat cagcggcgac cagcctttgc agcgggcgct aaccgtgctg cgtgacaaca
    17221 cacagccagg cgtcttttct gaaaaactct ctgccaaaga acgggaggaa gccctggcag
    17281 agccggatta ccagttactc acccgcctcg ggcatgaatt cgccccggaa aacagtaccc
    17341 tggcagtaca gaaagacaaa gaaagcacga tgcaggccgt gtatcagcaa ctcaccgagt
    17401 tgcaccgcta cctgctggca atccagaacg cgcctgtacc aggaaaatcg gcgctgaaag
    17461 ccgtgcagtt acggcttgat cagaacagca gcgatccgat attcgccacc cgccagatgg
    17521 caaaaacgct gcctgctccg ctcaaccgct gggttggcag gctggctgac caggcctggc
    17581 atgtggtgat ggtggaggct gttcattata tggaagtgga ctggcgcgac agcgtggtga
    17641 aaccgtttaa cgagcaactg gcaaataact atccgtttaa tccgcgttct gcacaggatg
    17701 cctcactgga tgccttcgaa cgcttcttta aaccggatgg catactggat accttctacc
    17761 agcagaacct gaagctgttt atcgataatg acctgagtct ggaggatggc gataacaacg
    17821 tcattattcg cgaagatatt attgcgcaac tggaaactgc gcagaaaatc cgtgacatct
    17881 tcttcagcaa acagaacggt ctgggaacat cctttgccgt ggaaacggta tcgctttcag
    17941 gcaataaacg ccgcagtgta ctgaaccttg acggtcagtt agtcgattac agccagggcc
    18001 gtaactatac cgcccatctg gtctggccta acaacatgcg cgaaggcaac gaaagtaagc
    18061 tgacgctcat cggcaccagc ggcaacgcgc cgcgcagtat cagcttcagc gggccgtggg
    18121 cgcagttccg cctgttcggg gccggacaac tgaccggagt acaggatggc cactttaccg
    18181 tgcgctttag cgtggacggt ggcgcgatga cctaccgtgt gcataccgac acggaagata
    18241 acccgttcag cggtgggttg ttcagccagt ttggtctgtc agacacactg tactgaggag
    18301 gcagggatga atagtaacgt actgacacaa actatcgtta ccggcagtga cccgcgcggg
    18361 ctgccggaat tcagcgccat ccgcgaggaa ataaacaaag ccagccaccc gtcacagcct
    18421 gagctgaact ggaaactggt ggagtcgctg gcgctggcga tttttaaagc caacggtgtg
    18481 gatttacaca ccgccaccta ctatacgctt gcccggacac ggacccaggg actggcggga
    18541 ttctgcgaag gtgcggaact gctggcggca atggtaagcc acgactggga taagttctgg
    18601 ccgcagggcg gcccggcgcg tactgaaatg ctggactggt ttaactcccg aaccggcaat
    18661 attctgcgtc agcaaatctc ctttgcggaa tccgacctac cactgatcta ccgcacagag
    18721 cgggcattgc agcttatctg cgataagctc cagcaggtgg aactgaagcg cgttccgcgc
    18781 gtggagaatc tgctctattt tatgcagaac acgcgtaaac ggcttgaacc ccagctgaag
    18841 agtaacactg agaacgccgc acagaccacg gtcagaacgc tgatttatgt cccggaaaca
    18901 caggcatctt ccacaccaga agcggtagtg cctcccctgc ccggcctacc tgagatgaaa
    18961 gtggaagtgc gcagtctgac agagaatccc ccacaggcca gtgtgataaa gcaaggcagt
    19021 acggtaagag ggtttatcgc agggatcgcc tgttcagtgg ctgtcgcctc agcattgtgg
    19081 tggtggcagg tctatccggt gcagcagcaa ctgttacagg ttaacgacac cgctcagggc
    19141 gcagcaacgg tgtggatggc ctcacctgaa ctcgaaaact atgagcgcag gctgcaacaa
    19201 cttcttgata cctccccggt acagccgctg gaaaccggga tgcagatgat gcgtgttgcc
    19261 gacagtcgct ggccggaaag cctgcaacag caacaggcct cgacacaatg gaatgaggca
    19321 ctcaaaaccc gcgcacagag tagcccgcag ttgcgtggct ggttgcagac ccgccaggac
    19381 ttacatgctt ttgcagatct agtgatgcag cgcgagaaag agggactaac cctttcctat
    19441 atcaaaaatg tcatctggca ggcggagcgg ggactggggc aggaaacacc cgttgagtct
    19501 ctgttgacgc agtaccatga tgcccgtgcg cagaagcaga atacagatgc gctggaaaaa
    19561 caaattaatg agcgactcga aggcgtgtta agccgctggc tgctgctgaa gaataacgtc
    19621 atgccagagg cggcaaccgg taccacggcc gaaaaataat tatttaatta aaggagatta
    19681 tcatggcaaa tataagttat ttatctttga gtggagagac acaagggcta atttcagcag
    19741 gttgctcaac gcttgattct gtcggaaaca aagcgcagcc agaacataaa gatcagatca
    19801 tggtttatgc gttaatgcat tccatctcaa gaagtcaaaa tgtcaatcat catgaattaa
    19861 ttatcaccaa accggttgat aaatcctcac cattgctggc aaaagcactt agtgacaatg
    19921 aaaaaatggc aatatgtgaa tttatactct accgaaccag taaagcgggt atttaccagc
    19981 cttattataa aataaatctg tcaaaagccc gaatatcaag tattgatttt gtcacaccgc
    20041 atgctgtcct ggaaaaagaa ctggaaccac aggagagaat tgcctttatt tatgaagata
    20101 tttcctggga gcatacgtta gccggtacga atgcaatgag taaatggcag gatcgtgtac
    20161 agtaacttac tgtaataaca taataaatta ctattgcgga gagataaaat ggctggttta
    20221 aggaataaca acaacacaca gaatgcacag tgggctgatt atgtgggtga cattcttcgg
    20281 ggggctcagc cgataaacca gctagtacct cagcaccctt atcttaacga cgtcccactc
    20341 attgatgagt taaggcatca gaacactcat catgttattc tcttgacact tgatgttgca
    20401 aaaaaaatac tcagcccaat aacatcattt gattacattc attttatcac tacacatcct
    20461 tcaggtataa aagatacact cgcctggtta gttaacgcga gtaaattaat gactgagttt
    20521 gatgacaatg ggaaaattat atttaacctt aatgccttaa agtatacaaa agcaagttac
    20581 tttgaaatac ttggagagaa atatataaaa atcacaacat ccagcccctg gcttttagaa
    20641 aagctcggta aatatatttt ctcttcacga gcgcctcaag ttctggaact cgctattggc
    20701 tggcgaggag ctttatctga atcaataaaa ggagttaagt tctgtatctg gttttcagta
    20761 gcctggagaa ccatagaatt tattatgagt tcagagcgag atctggttaa ttttcttggc
    20821 gatttttcaa tggatgtggc caaagcagtt atagccggtg gtgttgcaac cgctattgga
    20881 agcctggctt cttttgcctg tgttagcttt ggctttccag taattcttgt cggaggagca
    20941 attttactga cagggatagt gtgtacagtt gttttaaatg aaatcgatgc tcaatgccat
    21001 ttatcagaaa aattaaaata tgcaattaga gatggactaa aacggcaaca ggaacttgat
    21061 aaatggaaaa gggaaaacat gactccattt atgtatgttc ttaacactcc acccgtgata
    21121 tgaggattaa ccatgcgttt acatgttaaa ttaaaagagt ttctttcgat gttttttatg
    21181 gccatattat ttttccctgc attcaatgct tctt
//
LOCUS       DAZGSP010000087        20826 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZGSP010000087 DAZGSP010000000
VERSION     DAZGSP010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.087"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(83..1732)
                     /gene="actP"
                     /locus_tag="ACHK74_003602"
     CDS             complement(83..1732)
                     /gene="actP"
                     /locus_tag="ACHK74_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFP0222350.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1729..2043)
                     /gene="yjcH"
                     /locus_tag="ACHK74_003603"
     CDS             complement(1729..2043)
                     /gene="yjcH"
                     /locus_tag="ACHK74_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFP0222351.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2243..4201)
                     /gene="acs"
                     /locus_tag="ACHK74_003604"
     CDS             complement(2243..4201)
                     /gene="acs"
                     /locus_tag="ACHK74_003604"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFP0222352.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4594..6030
                     /gene="nrfA"
                     /locus_tag="ACHK74_003605"
     CDS             4594..6030
                     /gene="nrfA"
                     /locus_tag="ACHK74_003605"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFP0222353.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6075..6641
                     /gene="nrfB"
                     /locus_tag="ACHK74_003606"
     CDS             6075..6641
                     /gene="nrfB"
                     /locus_tag="ACHK74_003606"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFP0222354.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDIEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6638..7309
                     /gene="nrfC"
                     /locus_tag="ACHK74_003607"
     CDS             6638..7309
                     /gene="nrfC"
                     /locus_tag="ACHK74_003607"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFP0222355.1"
                     /translation="MTWSRRQFLTGVGVLATVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7306..8262
                     /gene="nrfD"
                     /locus_tag="ACHK74_003608"
     CDS             7306..8262
                     /gene="nrfD"
                     /locus_tag="ACHK74_003608"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFP0222356.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGVAVALIAMAIRQRKNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8282..10000
                     /gene="nrfE"
                     /locus_tag="ACHK74_003609"
     CDS             8282..10000
                     /gene="nrfE"
                     /locus_tag="ACHK74_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFP0222357.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            9993..10376
                     /gene="nrfF"
                     /locus_tag="ACHK74_003610"
     CDS             9993..10376
                     /gene="nrfF"
                     /locus_tag="ACHK74_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFP0222358.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10373..10969
                     /gene="nrfG"
                     /locus_tag="ACHK74_003611"
     CDS             10373..10969
                     /gene="nrfG"
                     /locus_tag="ACHK74_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFP0222359.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWENDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11311..12624
                     /gene="gltP"
                     /locus_tag="ACHK74_003612"
     CDS             11311..12624
                     /gene="gltP"
                     /locus_tag="ACHK74_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFP0222360.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12702..13391)
                     /gene="yjcO"
                     /locus_tag="ACHK74_003613"
     CDS             complement(12702..13391)
                     /gene="yjcO"
                     /locus_tag="ACHK74_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFP0222361.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13485..15632)
                     /gene="fdhF"
                     /locus_tag="ACHK74_003614"
     CDS             complement(13485..15632)
                     /gene="fdhF"
                     /locus_tag="ACHK74_003614"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15213..15215),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFP0222362.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15830..17296)
                     /gene="mdtP"
                     /locus_tag="ACHK74_003615"
     CDS             complement(15830..17296)
                     /gene="mdtP"
                     /locus_tag="ACHK74_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFP0222363.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPALKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17293..19344)
                     /gene="mdtO"
                     /locus_tag="ACHK74_003616"
     CDS             complement(17293..19344)
                     /gene="mdtO"
                     /locus_tag="ACHK74_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFP0222364.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19344..20375)
                     /gene="mdtN"
                     /locus_tag="ACHK74_003617"
     CDS             complement(19344..20375)
                     /gene="mdtN"
                     /locus_tag="ACHK74_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFP0222365.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20394..20669)
                     /gene="ytcA"
                     /locus_tag="ACHK74_003618"
     CDS             complement(20394..20669)
                     /gene="ytcA"
                     /locus_tag="ACHK74_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFP0222366.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4696 a         5514 c         5701 g         4915 t
ORIGIN      
        1 tttgtttaat attatgggaa aaggtgatgc atgctggaga ggaagagtat tccccggtca
       61 ggcgaccggg gaaggggaga gattaatgtg cgcggccttg ctcaacgcca aagccggtct
      121 gggagcggat aaactgtgca cggaacagtt cacgctcgcg cgcaccttcc gctgagttat
      181 cggttgccga gaagaaccag atgccgagga atgccacgct gatagagaac agcgccgggt
      241 attcatacgg gaagatggct ttttcgtgac caaggatctg tacccaaatc gtcgggccga
      301 ggatcatcag taccactgcg gtaatcagcc ccagccagcc acccatcatc gcgccacgag
      361 tggtcagttt cgaccagtac atagaaagca gaatgatcgg gaagttacag ctcgctgcga
      421 tggcaaacgc caggcctacc atgaaggcga tgttctgatt ctcaaacagc acgccgagga
      481 taatggcaat cacacctaaa atcagcacgg tgattttcga tacccgcagt tcttcacgtt
      541 cggtcgcgcc ttttttgaag acgttagcgt acaggtcatg cgaaaccgct gatgcgcccg
      601 ccagcgtcag accagcaacc accgcaagaa tggtggcaaa agcaaccgct gaaataaaac
      661 cgaggaacag gttgccgccc actgcattcg ccaggtgaac ggccgccatg ttgttaccac
      721 caatcagatg gcccgccgcg tctttatatt ccggattcgc accaaccagc atgatcgcgc
      781 cgaagccgat aataaaggtc agaatataga agtagcccat aaatccggtg gcgtagaaca
      841 cgctcttacg tgcttcgcgg gcatcgctga ctgtaaagaa gcgcatcaga atgtgcggca
      901 agcccgccgt accaaacatc agtcccagac ccagagagag cgcggagatc ggatctttca
      961 ccagcccgcc cggcttcatg atgtcgacac ctttcgggtg taccgccatc gcttcactga
     1021 acagattgtt gaagctaaag ccgacgtgtt tcatcaccat aaaggccata aagctggcac
     1081 cgaacagcaa cagcacggct ttgataatct gtacccaagt ggtcgccagc atgccgccga
     1141 acaggacgta catcatcatc agcacgccga ccagcaccac cgcaatgtga tagttaaggc
     1201 caaacagcag ctcgatcagt ttacctgcgc ccaccatctg ggcgataagg taaagcgcca
     1261 ccaccaccag agaaccacag gccgaaagaa tacgaatcgg cccttgtttc agacggtaag
     1321 aggccacatc ggcaaaggtg tagcgcccca ggttacgcag acgttcggca atcaggaaca
     1381 aaatgatcgg ccagcccacc aggaagccca gcgagtaaat taagccgtca tagccggagg
     1441 taaacaccag cgcggagatc cccaagaatg aggcggcgga catatagtcc ccggcaatcg
     1501 ccagcccgtt ctggaagcca gtgatattgc cgcctgcggt gtagtagtcg ctacgagaac
     1561 gtacgcgttt tgatgcccag taggtaatgc cgagcgtaaa cacgacgaaa atcaggaaca
     1621 taataatcgc ctgccagttc gttggctggc gctctacggc cccgctaata gcatccgcgg
     1681 cgttagctgc gaaagggagt gtggcggcaa gcgccgtcag aactcttttc atgatgcttg
     1741 tacctcatgc aggacttcat tattaagacg gtcgaattcg ccgttcgccc gccagatgta
     1801 gataccggtg agaacaaagg agatcacaat cactccaaca ccaattggaa taccgcgtgt
     1861 gacgctggtg ttcggattca gcggcgtgcc cagccagccg ggcgcgaagg cgatcagtaa
     1921 aataaagccg atataaactg ccagcataat aatcgacagg atggtggcaa accgttgccg
     1981 tttttcgact aactccctga aatgcgcatt gtcttctatc cgctgataaa tagtgccatt
     2041 catcacagat tctccagagg taatgtaggg attgttttaa ttcccgctcc cttatgggag
     2101 aaggttaacg ctcgggtaac ccttgccgaa tgtaggccgg ataaggcgtt tacgccgcat
     2161 ccggcaatca atgcctgatg cgacgctgtc gcgtcttatc aggcctacaa accgttaccg
     2221 actcgcatcc ggcaattgtg ggttacgatg gcatcgcgat agcctgcttc tcttcaagca
     2281 gcttctcgac tacgccagga tcggcaagcg tcgaggtatc gcccaggttg ctggtatcgc
     2341 ccgccgcaat tttgcgcaga atacggcgca taattttgcc ggagcgggtt ttaggcaggg
     2401 agtcggtcca gtgcagcacg tctggcgtcg ccagcgggcc aatctcttta cgcacccagt
     2461 tgcggacttc tgcgtacagt tctggtgacg gttcctcccc gtgattaagc gtgacgtagg
     2521 cgtagatcgc ctgaccttta atattgtgcg gaatacctac tacggcggct tcggcaatct
     2581 tcggatgcgc caccagcgcc gactcaatct ctgccgtccc cagacggtga ccggagacgt
     2641 tcagcacgtc gtccacacgc ccggtgatcc agtaatagcc atcttcatcg cgacgcgcgc
     2701 cgtcgccgct gaaatacata tttttgaagg tggagaagta ggtctgttca aaacgttcgt
     2761 gatcgccaaa cagcgtacgc gcctgacccg gccaggagtc ggtgatcacc aggctgcctt
     2821 cggtagcgcc ttccagcggg ttaccttcgt tatcgaccag cgccggttgc acgccgaaga
     2881 acggacgtgt tgccgaaccg gctttcagct cggtagcgcc aggcagcggc gtgatcatga
     2941 aaccgccagt ttcggtctgc caccaggtat cgaccaccgg acatttctcg ttgccgattt
     3001 ttttccagta ccactcccac gcttccgggt taattggctc gcccacggaa ccgagaatgc
     3061 gcagcgacga acggtcggtg ccttcgatcg ctttatcgcc ttccgccatc agcgcgcgga
     3121 tcgccgtggg tgcggtatag agaatattga cctgatgctt gtccaccacc tgcgccatac
     3181 gggcaggcgt cggccagttg ggtacgcctt caaacatcag cgtggtcgca ccgcaggcca
     3241 gcgggccgta cagcaagtaa ctgtgtccgg tcacccagcc cacatcggcg gtgcaccagt
     3301 agatatcacc cggatgataa tcaaagacat atttaaaggt cagcgccgcg tacaccagat
     3361 aaccgccggt agtatgcagc acaccttttg gcttaccggt agaaccggag gtgtagagaa
     3421 taaacagcgg atcttcggcg ttcatctctt ccgcctggtg ctgatcgctc gcttgctcaa
     3481 ccaggtcgtg ccaccacagg tcgcgccctt cctgccagtc aattttcccg ccagtacgct
     3541 tcagtaccac cacatgctct acgctggtga cgttcgggtt tttcagcgcg tcatcaacgt
     3601 ttttcttcag cggaatactg cgcccggcac gcacaccttc gtcggaagtg atcaccagtc
     3661 gtgagttgga atcaataatg cgcccggcaa cggcttccgg cgagaagccg ccgaaaatca
     3721 ccgaatgcac cgcgccaatg cgggcgcagg ccagcatcgc aaccgcggct tccggcacca
     3781 tcggcatata aatcgccacc acatcacctt ttttaatgcc cagctcgagc agggtattgg
     3841 cgaagcggca gacgtcgcgg tgcagctctt tatagctgat atgtttgctc tggctggcgt
     3901 cgtcgccttc ccagatgatg gcggtgcgat cgccgttttc ttgcagatgg cggtcaaggc
     3961 agtttgccgc cagattcagc gtgccgtcct cgtaccattt aatggacaca ttaccggggg
     4021 caaaggaggt gtttttcacc ttctggtaag gtttgatcca gtcaagaatt tttccctgtt
     4081 cgccccagaa ggtatcaggt acgttaatag attgttgata catcgcctcg tactgctgag
     4141 ggtttatcag gcaacggtct gcgatgttgg caggaatggt gtgtttgtga atttggctca
     4201 tgcttttgtt ctccttgtag gatgttaata atatgtggca taagcgttaa atgtaggggt
     4261 attggcagtt ttgtttagta tttgggcgac agatcacgca aaagtagaat tgtgcaaata
     4321 aacggcaggg taatttttga aggtcagcaa caaaagttga ttaattcttt gaggaacatg
     4381 cagttatgca tgctgttgaa aaagaggaag atactgacta actctaaagt ggtattttac
     4441 atgcacttac aattgattaa agacaacatt cacagtgtgg ttatttgtta cacatagggg
     4501 cgagcaatgt catgacagtg taggtgcggt tactgtcgtg aaaaacaata aaaaccgcca
     4561 ttgcaacaat ggcgcaattc ggatgaagcc cctatgacaa ggataaaaat aaacgcacgc
     4621 cgtatcttca gcttattgat tccttttttc tttttcactt ctgttcacgc tgaacaaacg
     4681 gctgctcccg caaaacctgt aactgtggaa gcgaagaatg aaacctttgc cccgcagcat
     4741 cccgatcaat atctctcctg gaaagccacc tcggaacagt cagagcgtgt tgacgccctg
     4801 gcggaagatc cacggctggt gatcctgtgg gcggggtatc ccttctcgcg cgattacaac
     4861 aagccgcgtg gacatgcctt tgctgtgacc gatgtgcgtg aaaccctgcg taccggtgcg
     4921 ccgaaaaacg ctgaagatgg tccgctaccg atggcatgct ggagttgtaa aagccctgat
     4981 gtggcgcgtc tgatccagaa agacggcgaa gatggctact tccacggtaa atgggcgcgc
     5041 ggcggcccgg aaatcgtcaa caacttaggt tgtgccgact gccataacac cgcctcacca
     5101 gagttcgcca aaggcaaacc ggagttaacc ctttcccgtc cgtatgcggc tcgcgcgatg
     5161 gaagccattg gtaaaccttt tgagaaagcc ggacgtttcg accagcaatc gatggtttgc
     5221 ggtcagtgcc atgtggagta ttacttcgac ggcaaaaaca aagcggttaa attcccgtgg
     5281 gatgacggca tgaaagtcga aaatatggag cagtattacg acaaaattgc cttctctgac
     5341 tggactaact ccctgtcgaa aacgccaatg ctgaaagcgc agcacccgga atatgaaacc
     5401 tggacagcgg gcattcacgg taaaaacaac gtgacctgta tcgactgcca tatgccaaaa
     5461 gtgcagaacg ccgaaggcaa actctacacc gaccataaaa ttggtaatcc gtttgataac
     5521 ttcgcccaga cttgtgcgaa ctgccatacc caggacaaag ctgccttgca aaaagtggtc
     5581 gcggaacgta agcagtcgat taacgacctg aaaatcaagg ttgaagatca actggttcac
     5641 gctcacttcg aagcgaaagc agcgctggat gcaggcgcga cggaagctga aatgaagcca
     5701 attcaggacg atatccgtca tgcccagtgg cgctgggatc tggcaatcgc ttcccacggc
     5761 attcatatgc acgcaccgga agaaggttta cggatgctcg gtacggcgat ggataaagcg
     5821 gcggatgcac gcaccaaact ggcgcgcctg ctggcgacca aaggcatcac ccatgaaatc
     5881 cagatcccgg atatctcgac caaagagaaa gcccagcagg ccattggcct gaacatggaa
     5941 caaatcaagg ccgagaagca ggacttcatc aaaacggtga tcccgcagtg ggaagaacag
     6001 gcacgtaaaa atggtctgtt aagccaataa cccagttccg cctcgcaagg ggcggaaaac
     6061 acaatggagt gaatatgagc gtattacgtt cgttattaac tgctggggtg ctggcgtcgg
     6121 gcctgttgtg gagcctgaac ggaattaccg ctacccctgc ggcgcaggca tctggtgatc
     6181 gctacgaagt tacccagcag cgtaacccgg atgccgcctg tctggactgt cataagccag
     6241 atatcgaagg tatgcatggc aaacatgctt ccgttatcaa cccgaataac aaactgccgg
     6301 tcacctgcac caactgtcac ggccagccat caccgcaaca ccgcgaaggg gtgaaagatg
     6361 tgatgcgctt taacgagccg atgtacaagg tcggagagca gaacagcgtc tgtatgtcct
     6421 gtcatctgcc ggaacagttg caaaaagcgt tctggccgca cgatgtccac gtaaccaaag
     6481 tggcgtgcgc cagctgccat tccctgcatc cacagcaaga tacgatgcag acgttaagtg
     6541 acaaaggacg gattaagatt tgcgtcgatt gccacagcga tcagcgcacc aatccgaact
     6601 ttaacccggc gtctgttccg ttgcttaagg agcagccatg acctggtctc gtcgccagtt
     6661 tctcaccggc gtcggcgtgc tggcaaccgt cagcggcacc gcagggcgcg tggtggcgaa
     6721 aacgttgaat atcaatgggg tgcgttacgg catggtgcat gacgagtcgt tatgcatcgg
     6781 ctgtaccgcc tgtatggatg cttgtcggga agtgaacaag gtgccggaag gcgtctcgcg
     6841 cctgacgatt attcgtagcg agccgcaggg cgaatttcct gatgtgaaat atcgcttctt
     6901 ccgtaagtct tgccagcact gcgatcatgc gccgtgcgtt gacgtctgcc cgaccggtgc
     6961 gtcttttcgt gatgccgcta gcgggattgt cgatgttaat cctgatctct gcgtcggttg
     7021 tcagtactgc atcgccgcct gtccgtaccg cgtgcgcttt atccatccgg tcacgaagac
     7081 ggcggacaaa tgcgatttct gccgtaagac caatttgcag gccggtaagc tgcccgcgtg
     7141 cgttgaggct tgcccgacga aggcgctgac gtttggcaat ctggacgatc ctaacagtga
     7201 gatttcgcaa ctgctgcgcc agaagcctac ttaccgctac aagctggcgc tgggaaccaa
     7261 accgaagctg taccgcgtac cgtttaaata cggggaggtg agccaatgac gcagacttcc
     7321 gcatttcatt ttgaatcgct ggtgtgggac tggccgattg ccatctacct gtttttgatt
     7381 ggtatttctg ccggtctggt gacgctggcc gtgctgttac gtcgcttcta cccgcaggcg
     7441 ggcggtgcag acagcacgtt gctgcgcacc acgctgattg tcggacctgg cgcggtgatc
     7501 ctcggtctgt tgatcctcgt cttccacctg acaagaccgt ggaccttctg gaagctgatg
     7561 ttccactaca gttttacctc ggtgatgtcg atgggggtga tgctgtttca gctctacatg
     7621 gtggtgctgg tgctgtggct ggcgaaaatc tttgaacatg atttgcttgc cctgcaacaa
     7681 cgctggttgc cgaagctggg gatcgtgcaa aaggttctga gcctgctgac gcccgttcat
     7741 cgcggactgg aaacgctgat gctggtgttg gcggtgctgc tgggggctta taccggcttt
     7801 ctgctgtcgg cgctgaaatc gtatccgttc ctcaataacc cgatcctgcc ggtgctgttc
     7861 ctcttctccg gcatctcgtc cggtgtggcg gtagcgctga tcgccatggc gatacgccaa
     7921 cgcaaaaatc cgcattccac ggaagcgcac tttgttcacc gtatggaaat ccctgtggta
     7981 tggggtgaaa tcttcctgct ggtggcgttt tttgtcggtc tggccctggg cgatgacggt
     8041 aaagtgcgtg cgctggtggc ggcattaggt ggcggtttct ggacgtggtg gttctggctt
     8101 ggtgtcgccg ggctggggct gattgtgcca atgctgctca aaccgtgggt caatcgcagt
     8161 tccggcattc ctgccgtgct ggcggcgtgt ggggccagtc tggtcggcgt gttgatgctg
     8221 cgctttttca ttctctacgc cggacagttg acggtggcgt aagccagaaa agaggtggtt
     8281 tctggacgta ttccttcctg aagtcggttt tctggcgttg ttgttaagtc tcggggtcaa
     8341 cgtgttgacc ccgttgacgg ccttcgcggg agtgcggttg cgctggcctg ccatgatgcg
     8401 actcacttgc atcggcattc tggcacagtt cgcgctcctg ctgctcgcct ttggcgtact
     8461 gacgtattgt tttctcatca gcgatttctc ggtcatttat gtcgcgcaac atagctatag
     8521 cctgctgtcg tgggaactca agctggcagc ggtgtggggc ggccatgaag gttcgctgct
     8581 gctttgggtg ctgctgcttt ccgcctggag cgcgctgttt gcctggcatt atcggcagca
     8641 aaccgatccg ctatttccgc tgacgttagc cgttttatct ctcatgctcg ccgcactgct
     8701 actgtttgtg gtgctgtggt ccgatccctt cgtgcggata tttccaccag caatcgaagg
     8761 ccgcgatctc aatccgatgc tgcaacatcc cggtcttatc tttcatccac cgctgcttta
     8821 ccttggctat ggcggtttga tggtagcggc gagcgtggcg ctggcgagtt tactgcgcgg
     8881 cgagtttgat ggtgcctgcg ccagaatttg ttggcgctgg gcactacctg gctggagcgc
     8941 attaacggcg gggatcatcc tcggttcttg gtgggcctat tgcgaactgg gctggggcgg
     9001 ctggtggttc tgggatccgg tggaaaacgc ctctttatta ccctggcttt ctgccactgc
     9061 gctgctgcac agtttgtccc tgacacgcca gcgggagatt ttccgccact ggtcgctgtt
     9121 gctggcgata gtaactctga tgctgtcgct actgggtacc ttaattgtcc gttctggcat
     9181 tctggtttcg gttcatgcgt tcgcactgga taacgtccgc gccgtgccgt tgttcagcct
     9241 gtttgcactg attagccttg cgtctctggc tctgtatggc tggcgagcgc gggacggtgg
     9301 tgcggtggtg cgtttttcgg ggttatcgcg ggaaatgtta atcctcgcta cgctgttgct
     9361 gttttgcgca gtgctactga tcgtgctggt gggaacgctt tatccgatga tttacggcct
     9421 gctgggctgg ggacgcctct ccgttggcgc gccgtatttt aaccgcgcga cgttaccgtt
     9481 tggcctgttg atgctggtgg tgattgtgct ggcgacgttt gtctctggca aacgcgtgca
     9541 gcttccggcg ctggtagctc atgcgggcgt gctgttattt gccgcgggga tcgtggtttc
     9601 cagcgtcagc cgtcaggaga tcagcctgaa tttacagccg ggtcagcagg tgacgctggc
     9661 aggatacacc ttccgttttg agcgcctcga tctgcaagcc aaaggcaatt acaccagcga
     9721 aaaagcgata gtggcactgt ttgaccatca gcaacgcatt ggtgaactga tgccggagcg
     9781 gcgtttttac gaagcacgtc gtcagcaaat gatggaaccg tcaattcgct ggaacggcat
     9841 ccatgactgg tatgcggtca tgggtgaaaa aaccggagcg gatcgttacg cttttcgctt
     9901 gtatgtacaa agcggtgtgc gctggatctg ggggggagga ttgttgatga ttgcgggcgc
     9961 attgttaagc ggatggcggg ggaggaagcg cgatgaataa aggacttctc acgctactgc
    10021 tgttatttac ctgttttgcg cgcgctcagg tcgtagacac ctggcaattc gccaatccgc
    10081 aacaacagca acaggcgtta aatattgcca gccagttacg ttgtccgcag tgccagaatc
    10141 aaaacttact ggaatccaac gcgccggtgg cagtcagtat gcgccatcag gtttacagca
    10201 tggtggcgga ggggaagaac gaagtcgaaa tcattggctg gatgaccgaa cgctacggag
    10261 attttgttcg ctataaccca ccgttaacgg gtcagacgct ggtgttgtgg gcgctgccag
    10321 tggtgttgtt actgctgatg gcactgatcc tctggcgagt gagggcgaag cgatgaaaga
    10381 gcccaaaata ccggtgaaaa tgctgacaac actcacgatt ttgatggtat ttctctgtgt
    10441 cggcagttat ctgttaagtc caaaatggca ggcggtacat gcggaatatc agcgtcagcg
    10501 cgatccgcta catcagtttg ccagccagca aaccccggaa gcgcagcttc aggcattgca
    10561 ggataaaatc cgcgctaatc cacaaaacag cgaacaatgg gcgttactgg gcgagtacta
    10621 tctgtgggaa aacgattaca gcaattcgct gctggcgtac cgtcaggcgt tgcaactacg
    10681 tggtgagaac gctgaactgt atgcggcgct ggcgacggtg ctttattacc aggccagcca
    10741 gcatatgacc gcccagactc gcgcaatgat cgacaaagcc ctcgcgctgg actctaacga
    10801 aatcaccgct ctgatgctgc tggcttccga tgcgtttatg caggcgaatt acgcgcaagc
    10861 catcgaacta tggcaaaaag tgatggatct caactcaccc cggatcaacc ggacacagct
    10921 ggtcgagtcg attaatatgg cgaaattgtt gcagcggaga tcagattaat catctggtga
    10981 tgaagattat gttattgagt tgtataaaaa ataatcgaat gaacatatgc caaaaataat
    11041 caccaatcag tattattgca gattaaacaa ataaaaatct ttccataaca aatggttatt
    11101 cattaatcct gccataaact cgcgttatta tgcattaatg cagcgaaaag ctctgttgtt
    11161 aaagggttgc gcaacatacc gcgcaaatga tactgatcat aagcgttaaa aaaatctaca
    11221 aaccaacgca acacaattca tgccctggca gtatgtcacg ttctcgcgtt tctgaacggg
    11281 gaacggcgct ccattgagga agttattctg atgaaaagta taaaattcag cctggcctgg
    11341 cagattctgt ttgctatggt gctgggcatt ctcctgggaa gctacctgca ctaccatagc
    11401 gacagccgcg actggctggt cgtcaatttg ctctctccgg cgggtgatat cttcatccat
    11461 ctgatcaaaa tgattgttgt gccgattgtg atctccacgc tggtggtggg tatcgcgggt
    11521 gttggtgatg ccaaacagct cgggcgtatt ggcgcgaaaa ccattattta cttcgaggtg
    11581 atcaccaccg tcgccatcat tttggggatc actctggcga acgtcttcca gcccggtgcc
    11641 ggggtggata tgtcgcagct ggcgaccgtc gatatctcga aatatcagag cactacggaa
    11701 gcggtacaaa gcagttccca cggcattatg ggcacgattt tgtcgctggt gccgacgaac
    11761 attgtggcgt cgatggcgaa aggcgaaatg ctgccgatca tcttcttctc ggtgctgttt
    11821 ggtctggggc tttcctccct gcccgcgacg catcgtgaac cgctggtgac cgtgttccgc
    11881 tccatctctg aaaccatgtt taaagtgact cacatggtga tgcgttatgc accggtgggt
    11941 gtgtttgcgc tgattgcggt gacggtggct aactttggtt tctcttctct gtggccactg
    12001 gcgaaactgg tgctgctggt gcatttcgcc attctgttct tcgcgctggt agtgctggga
    12061 attgtggcgc gcctgtgcgg gttaagcgtc tggatcctga ttcgtattct gaaagatgag
    12121 ctgattctgg cgtactccac tgccagctct gaaagcgtac tgccgcgaat tattgagaag
    12181 atggaagcct acggagcacc ggcgtcgatc accagcttcg tggtgccgac cggttactct
    12241 tttaaccttg atggttcgac gctgtatcaa agtattgccg ctattttcat cgctcagctg
    12301 tatggcattg acctgtccat ctggcaggaa atcattctgg tgctgacgct gatggtgacc
    12361 tcgaaaggga ttgctggcgt gccgggcgtg tcgtttgtgg tgctgctggc aacgctgggt
    12421 agcgtaggta ttccgctgga aggcctggcg tttattgctg gtgttgaccg tatcctcgac
    12481 atggcgcgta ctgcgctgaa cgtggtgggt aatgcgctgg cggtgctggt gattgccaag
    12541 tgggaacaca aatttgaccg caaaaaggcg ctggcttatg agcgtgaagt gctgggcaaa
    12601 tttgataaaa ctgcggatca ataattgaag attgccgggg ttatccaccc ggcaatgtgt
    12661 ggatgcctga tgcgacgctg gtgcgtctta tcaggcctac cttaaccgtt ggttaatttt
    12721 tcaaactctt cacacccggt atcaaaccct tccatacagc tcaggttcaa ccagtgcagc
    12781 gccttttgtt tgttcttctc gataaaacct tcctcaccgt ttaagaacat cattcccgcc
    12841 cagtactcgg aataaccggt tcgggaaatt gcagagctgc gtttgaaata ccaggttgcc
    12901 ttgtcatcgt cggccttaat gcccacgccg ttggcgtaaa tcaaaccaag cagcatttgg
    12961 gcatcgacgg cggagtcatt ctccagatct tccgaggcgt tttctaacag cgaaatcgcc
    13021 tttggataat ccggtttacc cgcttgagta tttaccagaa tatgcgccag cgttacttca
    13081 ccttctttac tgcccgcttg cgccgctttc tctgcaagaa cttttgcttg tggatagtcc
    13141 agactgaccg gattggtgat tttgatctgc gccagcagcg cgcaggcatc agcatcaccg
    13201 ctgtcggcgg ctttctgtgc ccaatattcg gctttgctca aatcgccgga gctaaaccag
    13261 ctgtcggcaa gaaaatattg tgcgcgtcgg tccccggcct ctgctgcctt taaatactgg
    13321 ctgccaggct cggagtcgtt cgcgtgggca aagaatgtca aaaacaacat caatgcgata
    13381 atttttttca ttttgataat acgattgggt agggagtaac cagtataaag agttcgaatg
    13441 gataaaaaaa cagcctccga aaggaggctg tagaaaggac tgtattacgc cagtgccgct
    13501 tcgcgcaggc gagttttcaa cttgttgtac tcgtcaatca cgtactgctc ggcggcgcgc
    13561 tgatcggcga tcggctcgac gcgcacggcg cagtatttgt attccggcgt tttcgtaatc
    13621 gggcttaagt tttcggtaac cagctcgtta caggcaccaa tccaccactg gtaggtcatg
    13681 taaatcgccc ctttgttcgg acgatcgctg acctgcgcac gggtgataat tttgccttta
    13741 cgcgagtgca cccaaaccaa tgcctcatct tcaataccca gacgtttggc gtcttcggta
    13801 ttgatttgtg cgtagccagg ttcatcagcc agcgccgcca gtgccgcaca gttaccggtc
    13861 atcgaacggc aagagtagtg accaacttca cgcaccgttg acagtaccat cgggtactcg
    13921 tcggtgagtt tgtcgattgg cgctacccag tcgcaggtga agaactgtgc cagaccgttc
    13981 ggggtatcaa acttctcttt aaacagataa gaagtcccct gatcggcatc tgaagtatcg
    14041 cggcaaggcc actgaatgaa gcccagttcg cccattttct cgtaagtcgc accgtagaaa
    14101 tccgggcaca gatgacgcaa ctcatcccag atctcctggg tgttgttgta gtgcatcgga
    14161 taacccatac gggtggcgat ttcactgatg atttgccagt ccgttttcag atcccatttc
    14221 ggttcaactg ctttgaagaa acgctggaag ccacggtcag ccgcagtaaa cacgccttca
    14281 tgctcgcccc acgacgttga cggcaaaatg acatccgccg ccgacgcggt tttggtcata
    14341 aagatgtcct gaacgataac cagttccaga tcttcaaagg ctttacgtac tgccgacagc
    14401 tccgcgtcag tttgcagcgg atcttcgccc ataatgtacg cggcacgcac ttcgccatgc
    14461 gctgcgcggt gcggcagctc gctgatgcga taaccggtat gagcaggcag gctttccacg
    14521 ccccaggctt tggcgaattt ctcgcggtta gccggatctt tcacgtactg gtatcccgga
    14581 tacgtatccg gcagcgcgcc catatcgcag gcaccctgca cgttgttctg accacgaacc
    14641 gggtttacac ccgcatgcgg cttaccgagg ttaccggtca gcatcgcgag gctggtcaga
    14701 gaacgcacgg tttccacgcc ctggtagaac tgggttacac ccatgcccca caggatggcg
    14761 gcgcttttcg cctgggcata catccgtgcc gcctgacgaa tctcactggc gctgacgccg
    14821 gtgatatctt caaccgactc cggcgtgtag ccttcaacga ttttacgata ctcttcaaag
    14881 ccttctgtac gggaagcgac gaacgctttg tcgtacagat tttcttcaat aatgacatgg
    14941 cccatcgcat tcaacagcgc gatgttcgag ccgtttttca gtgcaatgtg catgtcagca
    15001 atgcgcgcgg tttcaatttt gcgcggatcg cagacgataa ttttcgcccc gttacgttta
    15061 gcgttaatta cgtgattcgc cacgattggg tgggaatccg ccgggttgta cccgaaaacg
    15121 aacactaaat cggtattatc aatttcgtta atagcattgc tcattgcgcc attaccgacc
    15181 gattggtgca gacctgcaac cgatgggccg tgtcagacac gagcgcagca gtcaacgtta
    15241 ttggtaccaa taacggcgcg cgcaaatttt tgcattacat agttggtttc gttacccgta
    15301 ccacgcgagg agccggtcgt ctggatggca tccggaccgt acttctcttt gatggcgctc
    15361 aggcgctcgg caacgtaatt cagtgcctca tcccaggaaa caggttcgag tttgccgcca
    15421 cgctggcgac ggatcatggg ggttttcagg cgcggggtca ggatctgggt atcgttaatg
    15481 aaatcccagc cataataacc cttcagacac agggtaccct ggttggtttt cccctgcgct
    15541 gcctccgccc ggacgatttt gccgttatcg acgaccaggt tgattttgca acctgatgcg
    15601 caataggggc aaaccgtgac gacttttttc atcggtctcg ctccagttaa tcaaatcacg
    15661 catacgcgct ctcgactaca gtatgcatct tttatgccac attttatttg gggtcattcc
    15721 ctgatattac gggcactatt tattcaaaac tctgacgaaa aacaggctgt cgtcagtttt
    15781 gacgtgacga aacgaaatac cgcgtgacag ccatcacgcg gcagacattt tattttttct
    15841 cgacgacggg agccgcctga tacccgccgc ccagcgattt catcaactga atgctttgga
    15901 tcacccggcg gctgtccagc atcagtaatg acatctcttc ggcaagtact ggcaaccggg
    15961 cttcagtggc ctgtaagcgg ctggttaagc cgcgctgata ggcggcctcg gcagcgcgct
    16021 gggtgaagcg cgttgcttcc acgcgttcag cctgcatttc tcgctcgtcg ttgagcgttt
    16081 gcagacgcgt gccgttgacg gcaacgtcac gcaccgcgtt cagtactgac tggttgtaac
    16141 gttcaatcat catgttgctg gcggcgcgcg tgccttcgag attggcattc aaccgtccac
    16201 cgtcaaacag cggcaatttc agagccggga tgaagttgaa ctggcgactg gtttttttga
    16261 ataaggtatc caggtggatg gcatcaaggc cgaaaaacgc tttgatatca aagctcgggt
    16321 agaacagcgc ccgcgcggaa tccacctgat ctaatgacgc ctgaacatac cagcgcatgg
    16381 cttgcagatc cgggcgtctg gcgagcaact cataagagag tgtcgccgga atgccggtct
    16441 ggactcgtgg taatgccacc ggtttgatct ccggcatatc gctggccccc gcgccaatca
    16501 atgcacgcag agattctcgc gtttcggtga tttgcccttt gacggcagca atttgtttat
    16561 cgaccgccag aatctgtgcc cgcgcgccgt ggaaaggcac ttgtgcttcc agaccgtgcg
    16621 ccactttact ctggtgcgct ttcaccgcgt aatcaatcac atcgcgagtt tgttctaaca
    16681 gatcgagcat ctgatagctg gcctgcatac tgtaataaag ctgcgctacg cccgtggtca
    16741 gcgatagctc tactgctgcg gtttctgcca gcgcggcatt atgcgcgcca atggcggcgg
    16801 caaccgctga gcgatgcaca ccccacaaat caagatccag tccggcaaac aaacctactg
    16861 tggcttccgt atagtacggc ccgtccatac ccagtgcggg cgcatccatc gcataagggc
    16921 ttaaaaagcc gttcgccgag acgcgttggc ggttgagcat ccctaacgct gcgacctgta
    16981 attgtgaacc ggcatctaac aaatcggcct gggactgcgc tttttcttcc cgcagtttcg
    17041 cttcggcgag ggtgtgtgaa ccacttagcg tccgttggat cagcgcatcc agctgcgggt
    17101 cattgagttg tttccaccac tgcgcctgcg gccagccgga gctggcaaga tgaatatcgt
    17161 cggccagttt gatttgttcc ggtttgagct gttgatgagg tgcagaatcc ttacgtacca
    17221 gggcacagcc ggaaatcagc gtcgtgctgc cgagaatgct gcacaacagc agacgtgaaa
    17281 gttgacgatt gatcattgcg tggctccttg tgcctgttcc gtggcgggcg ttaatgccgg
    17341 ggctgtccag tctggcaagc gggcgaaaag ctggcagacg tgttgctcct gttttaataa
    17401 ggtgggcagg atggcctgcg aggcgggtgt ctcttcaagc gttatttgag gagaacggct
    17461 gagtgcggtt gccagaccgg cagcgtattt ctccagggcg tcggcaaact gagctgctct
    17521 gtccggctgt agtgcgttat ccagcgcctg cgccgagttc caggtggcat cccaggcgtg
    17581 aagaatatcg cggccctgac gaataaccgt ttgcgaacgt tcaatcagta aggcgcgttc
    17641 ttcgctgtcc agttgacgct ccagcgccac gcgttggcac atctcttcac aggcattaaa
    17701 cgccgcatgc agaccgatac gaatttgcag ataagtgcgc agagccgtga cttcctgctg
    17761 gcgtgggatc cgcattactt tacttaacat acccagcgtg ccagccagtt tttgcggcag
    17821 tgtgcgcgct tcactttcag gccagacaaa ggtgtaaatc accgcggaca ccacggtacc
    17881 aatgatgata cccatggcgc gatcgcgaat ttccaccagg tcgtacaccg ggccaaaaac
    17941 gttttcgagc gtggcgagcg cgaaggtgac caccatctgt gtgccgatat aagaagagcg
    18001 ttcagagctg gtggcaatcc atgcgcccaa caggaaaatc ggtgccagca caaacagcaa
    18061 ttcgacaata ttgtccagcc agggcatgac cagtagcgtg aataacagcg ccagaatcgc
    18121 gccgcaaaag gccccgccaa aacgcagcac catcttctgg tacgacgaac cgacatttgg
    18181 gttagcgacg atcacgcatg tcagcataca ggtgtgaatg ccttcccagt ccacgccgct
    18241 gtagaaggtg taacagatca aacaggcgag cagtgttttt accgcgtagc gcatatagtc
    18301 tggattggta aaagcatcgg cgaccattga tggcggtttg gcggcgggcg ttggcggcgt
    18361 attcgggtcc atctggccca gttgtaacaa cgtctggcag atattctcca ggttacattc
    18421 ccgtgccgcc atcgcttcac tttcagtgat ccgccagtcg ctttgccagc actgaccttc
    18481 agcaacggca tgctgcagct tattgatttc tgaggctaat ttttgccgaa attcaataat
    18541 tgcctgagaa tcagcaaaag aggtgggatc gtagcgattc agcgtcgagt aaatgtaggt
    18601 taccgttgcc acgcagcttt gccaccatgc gctttgagtt cgccagttgg catcgtccgc
    18661 gagacaaaag acattcagtt tttgcagcgc cagcgcctct ctttcaatcc gcgtttcggg
    18721 tagcggtgcg aggctgtccg tcaggtgact aatggcatca tcaagccgat cattaagcgc
    18781 ttgatgcatt tgcgtaatgg cacgactggg aaaccacagc acgccgatta acgtcatcag
    18841 caaggttgga tagaggccaa caacgataca ccacagcgtt aagcgcacga ccacttccgg
    18901 atagtcgagc atggcgggga aggtttgccc gtaaatggcg acaatggcga cagcgaaaaa
    18961 gaccagtccc agccgatggg tgcgcatcaa aaacatacag cccatcagga tcggcccggc
    19021 gatgatcaat cggatcaacg gttcgccgta tgaccatttg tagatcaaaa acaggctgcc
    19081 gatctccagc accgtggcaa ccacaaacaa gatcgcgaca aatttggtgt aaaacgcgtt
    19141 cgactgaata ccgtaaaaca gcactgccag cgataacgcc acaaaaggga tctcaaaggt
    19201 catcgagatc agaatcacca gcaggcagcc tacccagagt tgtacggtct gcggcacgcg
    19261 gcctggtcgc cgctcgctta actcttcatg aaagaacgcc agcagcctga ccaccggtaa
    19321 tggcagggag ttgagcgcgc tcattattgc ggctcaagat tagcgactgc cgaagcgccg
    19381 atgcggaaca tttccgggtc aggtttatcg accatgattt tgaccggaaa acgctgggca
    19441 acgcggaccc agttaataga gcgagaaact ttcggcaggc cgcccagcac caggccgccg
    19501 tcatccggta gcacgccgta gccaatcgaa tccactttac cctcgaaggt tttgccgctg
    19561 tcactcatca ggcgaatcgt tgcgggtgta cctgagcgaa tatttttcag atcggtttcg
    19621 cggaagttgg cgatcacgta ccagtgacga gtgtcgatta gggtaaaaat agggcgcatg
    19681 gcagaagcaa attgcccgac ggaggttttg agggaaatga cccggccatc aaacggcgcg
    19741 cgaacggtcg ccatttccag atgcagtttg gtcagggcaa tatccgcttc gaccgccgca
    19801 cgctgggcaa ctaatgcatc cacgccgctg acggcgctgg cggctgactg cgcctgcaac
    19861 aatacagcat taagatcggc ttctgcagcg cgctgcgcgg tcctcgcacg gtcaacgtct
    19921 tccgctgaga caaaaccttc tttcagtaat ggctcggtgc ggcgtaatgt atctgtggcc
    19981 tgtttcgcgg cggcacgggc tttttctacc gtggcgttaa ccgagtcggc accaaactgt
    20041 tgcgcgtcaa cgctacgctg ggtgagcata atttgcttat ccagcgccgc gagggaggct
    20101 tcagctttcg ccagattggc ttcgtacggg cgcgggtcaa tgcggaacag caaatcgccc
    20161 tgtttgactg cctggttgtc ggtgaccgcc agttctacaa tgcgaccgct gacttccggc
    20221 accacatcaa tggtatctgc tgacgcgtaa gcgtcattag ttgatggcgc actgtctacg
    20281 cgccagataa cgaaaacaag ggcaaccagc gccaacgcaa ccactaacag agcagggaat
    20341 ttactgcgag gagctttttt cggcgtactt tccatgagcg cgtctcaaat agattagaaa
    20401 aatgccagcc agaacagcat ggcgtagaga gcaaaaaggg cggtataaat aattccgaca
    20461 aatgccagat tgatatttgc ccgctgaata actcgcctcg tgatgagcat caaaataagg
    20521 ctggcaatgg cgcagaaaaa ccagctggga taataagcgc cgatcaccgg gattgccgga
    20581 gataatgagc atcccgaaac catgaagacg ggaattatcc aggctgtttt tttgagttgc
    20641 atcgccatgc gagagagaac tgttggcata ttaaatacat cctgtctgct gcgaacaata
    20701 tctgtagtga atatcaacat ccattgaagg cgtcagtatg gctattttct tgatgaataa
    20761 aaatagcttt attcagccac cgcattcata acttatatat tttatttatt aattggctgt
    20821 atatat
//
LOCUS       DAZGSP010000088        20812 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZGSP010000088 DAZGSP010000000
VERSION     DAZGSP010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.088"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            84..860
                     /gene="exuR"
                     /locus_tag="ACHK74_003619"
     CDS             84..860
                     /gene="exuR"
                     /locus_tag="ACHK74_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFP0222367.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1100..1183
                     /gene="yqiM"
                     /locus_tag="ACHK74_003620"
     CDS             1100..1183
                     /gene="yqiM"
                     /locus_tag="ACHK74_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFP0222368.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1205..1867
                     /gene="yqjA"
                     /locus_tag="ACHK74_003621"
     CDS             1205..1867
                     /gene="yqjA"
                     /locus_tag="ACHK74_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFP0222369.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1871..2254
                     /gene="mzrA"
                     /locus_tag="ACHK74_003622"
     CDS             1871..2254
                     /gene="mzrA"
                     /locus_tag="ACHK74_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFP0222370.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2401..2769
                     /gene="yqjC"
                     /locus_tag="ACHK74_003623"
     CDS             2401..2769
                     /gene="yqjC"
                     /locus_tag="ACHK74_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFP0222371.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2807..3112
                     /gene="yqjD"
                     /locus_tag="ACHK74_003624"
     CDS             2807..3112
                     /gene="yqjD"
                     /locus_tag="ACHK74_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFP0222372.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3115..3519
                     /gene="yqjE"
                     /locus_tag="ACHK74_003625"
     CDS             3115..3519
                     /gene="yqjE"
                     /locus_tag="ACHK74_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP0222373.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3509..3808
                     /gene="yqjK"
                     /locus_tag="ACHK74_003626"
     CDS             3509..3808
                     /gene="yqjK"
                     /locus_tag="ACHK74_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFP0222374.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3994..4386
                     /gene="yqjF"
                     /locus_tag="ACHK74_003627"
     CDS             3994..4386
                     /gene="yqjF"
                     /locus_tag="ACHK74_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP0222375.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4456..5442
                     /gene="yqjG"
                     /locus_tag="ACHK74_003628"
     CDS             4456..5442
                     /gene="yqjG"
                     /locus_tag="ACHK74_003628"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFP0222376.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5735..6100
                     /gene="yhaH"
                     /locus_tag="ACHK74_003629"
     CDS             5735..6100
                     /gene="yhaH"
                     /locus_tag="ACHK74_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP0222377.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            6342..6698
                     /locus_tag="ACHK74_003630"
     CDS             6342..6698
                     /locus_tag="ACHK74_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP0222378.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(6749..7645)
                     /gene="yhaJ"
                     /locus_tag="ACHK74_003631"
     CDS             complement(6749..7645)
                     /gene="yhaJ"
                     /locus_tag="ACHK74_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFP0222379.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            7750..8451
                     /gene="yhaK"
                     /locus_tag="ACHK74_003632"
     CDS             7750..8451
                     /gene="yhaK"
                     /locus_tag="ACHK74_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFP0222380.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            8474..8638
                     /gene="yhaL"
                     /locus_tag="ACHK74_003633"
     CDS             8474..8638
                     /gene="yhaL"
                     /locus_tag="ACHK74_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFP0222381.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8850..10160)
                     /gene="cyuA"
                     /locus_tag="ACHK74_003634"
     CDS             complement(8850..10160)
                     /gene="cyuA"
                     /locus_tag="ACHK74_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFP0222382.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10188..11519)
                     /gene="cyuP"
                     /locus_tag="ACHK74_003635"
     CDS             complement(10188..11519)
                     /gene="cyuP"
                     /locus_tag="ACHK74_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0222383.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(11794..13158)
                     /gene="tdcG"
                     /locus_tag="ACHK74_003636"
     CDS             complement(11794..13158)
                     /gene="tdcG"
                     /locus_tag="ACHK74_003636"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP0222384.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(13230..13619)
                     /gene="tdcF"
                     /locus_tag="ACHK74_003637"
     CDS             complement(13230..13619)
                     /gene="tdcF"
                     /locus_tag="ACHK74_003637"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFP0222385.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFSTINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13633..15927)
                     /gene="tdcE"
                     /locus_tag="ACHK74_003638"
     CDS             complement(13633..15927)
                     /gene="tdcE"
                     /locus_tag="ACHK74_003638"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFP0222386.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15961..17169)
                     /gene="tdcD"
                     /locus_tag="ACHK74_003639"
     CDS             complement(15961..17169)
                     /gene="tdcD"
                     /locus_tag="ACHK74_003639"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFP0222387.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(17195..18526)
                     /gene="tdcC"
                     /locus_tag="ACHK74_003640"
     CDS             complement(17195..18526)
                     /gene="tdcC"
                     /locus_tag="ACHK74_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFP0222388.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18548..19537)
                     /gene="tdcB"
                     /locus_tag="ACHK74_003641"
     CDS             complement(18548..19537)
                     /gene="tdcB"
                     /locus_tag="ACHK74_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFP0222389.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19636..20574)
                     /gene="tdcA"
                     /locus_tag="ACHK74_003642"
     CDS             complement(19636..20574)
                     /gene="tdcA"
                     /locus_tag="ACHK74_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFP0222390.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         5376 a         5422 c         4944 g         5070 t
ORIGIN      
        1 accaaattta tatatgacca atggcatacc catgaataaa gtggtatgat aacttcgtcc
       61 gcattccact ccgggagacg catatggaaa tcactgaacc acgccgtttg tatcaacaac
      121 ttgccgctga cctgaaagag cgcatcgaac agggcgtcta tctggtaggt gataaactgc
      181 ctgcagaacg ctttattgcc gatgaaaaga acgtcagccg tacggtagtt cgtgaagcca
      241 tcatcatgct ggaagttgaa ggctatgtcg aagtgcgtaa aggttcaggt attcatgtgg
      301 tttccaacca gccgcgccat cagcaggcag ctgacaataa tatggaattc gccaattacg
      361 gtccgtttga gttgcttcag gctcgccagc tcatcgaaag taatattgcc gaattcgcgg
      421 caactcaggt aacgaaacag gacatcatga aactgatggc cattcaggaa caggcgcgcg
      481 gcgaacaatg cttccgtgat tccgagtggg atttacagtt ccacattcag gtagccctgg
      541 cgacgcagaa ctccgccctg gcggccatcg ttgaaaaaat gtggacacaa cgtagccata
      601 acccgtactg gaaaaaactg cacgaacaca ttgattcccg taccgtcgat aactggtgtg
      661 atgaccacga tcaaatcctc aaggcgctga ttcgcaaaga tcctcatgct gctaagctgg
      721 caatgtggca gcacctggaa aacaccaaga tcatgttatt caacgaaacc agcgacgact
      781 tcgagttcaa tgccgaccgc tatctattcg ccgaaaaccc ggtggtgcat ctcgatacgg
      841 ccaccagtgg cagtaaatga aatacctgtc cgacaggcgc tgcgacgcgc ctgtttgtct
      901 cgtcggtaag cgaaaagtat aaagtgtcag cctgtgtaaa tcctctcgcc accctcccct
      961 gcattcagca aaatcagact caacggacat gtaattttga taacgaacaa cgttgacctt
     1021 tgttacaatt agattcaatt tgaatttatg tttttgaatg ctttcttatc tcacgattta
     1081 acagggaata gttcaggctg tgttgatgta tcaaacccgc agaacatacc aaaacagcaa
     1141 taacattgcg gtagtgcatc ttttaaaacc agcgtggcgt taaccgattc accaggaata
     1201 atgaatggaa cttttgaccc aattgctgca agccctgtgg gcgcaggatt ttgaaaccct
     1261 ggccaatcca tcgatgattg gcatgttgta ttttgtcttg tttgtaattt tgttccttga
     1321 aaacggcttg cttccagcgg cctttttacc gggcgacagt ttactggtat tggtcggcgt
     1381 gttgattgcg aaaggcgcga tgggttaccc gcaaacgatt ctgctgctga ccgttgccgc
     1441 cagcctcggc tgctgggtca gctatattca ggggcgatgg ctgggcaata cccgcaccgt
     1501 acaaaactgg ctatctcatt tacccgcgca ttatcatcaa cgcgcacacc atctttttca
     1561 taaacacggt ttatcggcgc tgttaattgg tcgctttatt gcgtttgtca gaacactgct
     1621 gccgacgatt gccgggttat cagggctgaa taacgcgcgc tttcagtttt tcaactggat
     1681 gagcggtctg ctgtgggtat tgatcctgac aactctgggt tacatgctcg gcaaaacgcc
     1741 ggtattttta aagtacgagg accagctgat gtcatgcctg atgctgctcc cggtggtgct
     1801 gctggtgttt ggcctggcag gttctctggt cgtgttatgg aaaaagaaat atggaaatcg
     1861 ggggtaaggg atgcaaatac ctcgcatgtc gcttcgccag ctagcctggt ccggcactgt
     1921 tttacttctg gtcggcacgc tgttgctggc ctggtccgcg gttcgccagc aagagtctac
     1981 gctggcgatt cgtgccgttc atcaaggcac aacgatgcca gacggttttt cgatctggca
     2041 tcaccttgac gctcatggca ttcctttcaa aagtatcacc cccaaaaacg acactctgtt
     2101 aattacattt gactccagcg accagagcgc cgccgcaaaa gcggtcctcg acagaacatt
     2161 gccccatggc tacatcattg cgcagcagga caataacagt caggctatgc agtggctgac
     2221 ccggttacgg gataactctc atcgcttcgg ataacttcct gttatccgaa acatatcact
     2281 cactttggtg atttcaccgt aactgtctat gattaatgag gcggtggtta acactaccgc
     2341 ttcgttgtct ggacaatcgt tcctttgtaa taggtccgaa tcacaatgga aggttcaaga
     2401 atgaaatacc gcatcgcttt agctgtttct ctctttgctc ttagtgccgg tagttatgcc
     2461 actaccctgt gtcaggaaaa ggagcaaaat atccttaagg agatcagcta tgccgaaaaa
     2521 caccaaaacc agaatcgtat tgacggtctg aataaagccc tgagtgaagt ccgggccaac
     2581 tgttcagata gccagctgcg tgccgatcat cacaagaaaa tcgcaaagca gaaagatgag
     2641 gtggcggaac gccagcaaga tttagccgag gcgaagcaaa aaggcgatgc cgataagatt
     2701 gccaaacgcg aacggaaact ggcagaagcg caggaagagc tgaaaaagct ggaagcgcgc
     2761 gactactaac tcacaatagt cactacttac tcacctggag aaaactatgt cgaaagaaca
     2821 cactacggaa catctgcgtg ctgagttgaa atccctttcc gatacgctgg aagaggtgct
     2881 tagctcatct ggcgagaagt cgaaagaaga gttgagtaag attcgtagca aagcggagca
     2941 ggcactgaaa cagagccgtt atcgcctggg tgaaaccggt gatgccatcg ccaaacaaac
     3001 ccgtgtcgcg gcggcgcgtg ctgatgagta tgtgcgtgaa aatccgtgga ccggcgtggg
     3061 cattggcgct gcaatcggtg tagtgctcgg cgttctgctg tcgcgtcgtt aattatggcg
     3121 gacactcatc acgcgcaagg gcccggtaaa agcgttctgg gcatcgggca gcgaattgtt
     3181 tccatcatgg ttgaaatggt ggaaacacgt ctgcggctgg cggtggtgga gctggaagag
     3241 gaaaaagcta atctctttca acttttactg atgctgggcc tgacgatgct tttcgctgca
     3301 tttggtctta tgagcctgat ggtgctaatt atttgggcgg ttgatccgca atatcgcctg
     3361 aatgcgatga ttgccaccac cgtggtgttg ctgctactgg cactaattgg cggtatctgg
     3421 acgttacgta aatcgcgtaa atccaccttg ctgcgccata cacgccatga gctggcaaac
     3481 gatcggcaac tgctcgagga ggagtcccgt gagcagtaaa gtcgaacgtg aacgacgtaa
     3541 ggcgcaactg cttagccaga tccagcaaca acggctggat ctttccgcca gtcgtcgtga
     3601 atggctggag gcaacaggcg cttacgatcg tcgctggaat atgctgctaa gtctgcgctc
     3661 ctgggcgctg gttggcagta gcgtgatggc gatctggacg attcgccatc ctaatatgct
     3721 ggtccgctgg gccagacgcg gttttggcgt atggagcgcc tggcgtctgg ttaaaacgac
     3781 cctcaagcag caacagcttc gcggttaact tccctctggc cggagcaatt ccggccttat
     3841 ccctcaaatt tcttgaagat ttttgacagt tttccttgct aacaatcatc attcaccacg
     3901 tttatgattc tctccatcga cagcaacgac gctaataccg cgcccttgca caaaaaaaca
     3961 atcagcagcc tgagtggcgc agtggagagt atgatgaaaa aattagaaga tgttggtgta
     4021 ctggtagcgc gcattttaat gccgattctg tttattaccg caggctgggg aaaaatcacc
     4081 gcttactctg gtacccaaca atatatggaa gcaatgggtg tcccgggttt tatgctgccg
     4141 ctggtgattc tgcttgagtt cggtggtggt ctggcaatcc tgttcggttt cctgactcgc
     4201 accacagccc tgtttactgc gggctttacg ctgctgacgg catttttatt tcacagcaac
     4261 tttgctgaag gcgtcaactc gctgatgttc atgaaaaacc tgacaatttc tggcggattc
     4321 ctgctgctgg caattaccgg tccgggcgcg tatagcatcg accgcctgct gaataaaaag
     4381 tggtaagcac gcgctatact taacgataaa aaacgaggag gaagctcctc gtttttgcta
     4441 ttggaggaga gaaaaatggg gcaactgatt gacggcgtct ggcatgacac ctggtacgat
     4501 accaaatcta ccggcggtaa atttcaacgt tcagcttccg catttcgtaa ctggctcact
     4561 gccgatggcg cacctggccc cactggcaca ggcggtttta tcgcagagaa agatcgttat
     4621 catctctatg tttcactcgc ctgcccgtgg gcgcaccgca cgctgatcat gcgcaaactc
     4681 aaaggactgg aaccgtttat ttccgtttcc gtagtgaacc cgctgatgct ggaaaacggc
     4741 tggacctttg atgacagttt tccgggagca accggagata cgctctatca acatgaattt
     4801 ctgtatcagc tttatctcca cgccgatcca cactacagcg gacgagttac tgttcccgtg
     4861 ctgtgggaca aaaagaacca caccatcgtc agcaacgaat cagcagaaat catccgcatg
     4921 ttcaataccg cgtttgatgc gctgggcgca aaagcgggtg attactaccc accagccctg
     4981 caaacgaaaa ttgacgaact taacggctgg atttatgaca ccgttaacaa cggcgtgtat
     5041 aaagctggtt ttgccaccag ccagcaagcc tacgacgagg cggtggcgaa agtgtttgaa
     5101 tcgctggcgc gactggagca gattttaggc cagcatcgtt acctgaccgg caaccagcta
     5161 accgaagccg atattcgcct gtggaccacg ctggtccgtt ttgatccagt gtatgtgacc
     5221 cacttcaagt gtgataagca ccgcatcagc gattacctta atctgtatgg cttcctgcgt
     5281 gatatctacc agatgccggg aattgccgaa acagtcaatt tcgatcacat ccgtaatcat
     5341 tacttccgca gccataagac catcaaccct acggggatta tttcaattgg tccatggcag
     5401 gatctcgatg aaccgcatgg acgagatgtt cgcttcggtt aaaaaaaggc accttacgag
     5461 gtgcctctga taattagcta agtgcatgtt gttcatgtcg gatgcggcgt gaacgcctta
     5521 tcaaacctac aaaacattta tattcaatat gttgcaatga gcgcgcaggc cagataagcg
     5581 tagcgcatct ggcgattttg cgcttgtcat cagtcttagc gcgccttcac agcactttat
     5641 taattccttg aacgaatatt tactgccatt tcattcacgt ctattcttaa tttgctgctt
     5701 taaaaacaag tgattgagca aaattgaggc aaaaatggac tggtatctga aagtactaaa
     5761 aaattatgtc ggtttccggg gtcgtgcgcg gcgcaaagag tactggatgt ttattctggt
     5821 caacatcatc tttacgttcg tgctggggct gctggataaa atgttaggct ggcaacgcgc
     5881 tggtggcgaa ggcatcctga cgacaatcta cggtattctg gtgtttttac cgtggtgggc
     5941 agttcagttc cgccgcctgc acgacaccga ccgctcggcg tggtgggcac tgttgttctt
     6001 aatcccgttt atcggctggc ttattatcat cgtctttaac tgccaggcgg gtacgccagg
     6061 cgaaaaccgc tttggaccag atccgaaact ggaaccttaa aacaaatctg ctcgccggat
     6121 gtctgactca tccggctttt atcatcatgt tgatgaatga ataatttact aatgtttatt
     6181 taaaatattt caatgtcgtt atataaatta cctataaaaa acaaccatgt atttatctta
     6241 aatatcaaaa cctttataac atccgaaaaa gaaagatacg aatagcattt acacaatacc
     6301 cggaatagac tttcgtcact tttaattaaa gggatgtttt tatgcagtgg tatttagccg
     6361 tattaaaaaa ttatgttggt ttctctggtc gcgcacgtcg taaagagtac tggatgttta
     6421 ctctgattaa cgccatcgtc ggcgctatta tcaatgtcat tcaattgatt ttaggtttag
     6481 agtttccatt cctttctctc atttacctgg cagcaacaat cattccggtc attgctctgt
     6541 gtgttcgtcg tctgcacgat actgaccgtt caggtgcgtg ggcgcttctc tatctggtac
     6601 cgatcattgg ctggttagtt ctgtttgtat ttgcctgttt agaaggcaat tctggcagca
     6661 atcgttacgg aaacgatcct aagtttggtt caaattaatc ttagaattgg ggcgacattt
     6721 cgcccctttt tattaataat aacaaatatt attttccgct aaaaagtttg ggaatttccc
     6781 gcagacacca ggatttcgct tcccccatac tgtcacgacg ccaggccata ataatatcga
     6841 tctcgctggt cgattccggg ctgacgacac gcaaccgccc ttccgcaata tctttttcga
     6901 ccatcggata cggcatcgtc gccacgccaa gccccgccag taatgcctga cgtttatctt
     6961 caatcgtgct caccgttaag cgcggctgtt tgtccagcag ctgtacggtc aacaccgggc
     7021 gctcacgagc ggtatccgct accgcaattc cacgatattt cacgcgcgtc acttcagata
     7081 acggttccgg ctcctgatga atcgggtgat caggcgcggc aacgtagacg ttcattaagg
     7141 tatagagctt gcgcgagttg atctccgacg aggaacgaaa atgcatatcc ggcgcgataa
     7201 cgatatccgc ccgcccctgc tccagtcgtt cccacgcccc cgccagcact tcggtgatga
     7261 ttgccagttg ggtattggct tttgccgcca gtttgtcgat taacgggaaa aaggcaggtg
     7321 tcggtaccag cgcctcggtc acaatggtga gatgcgtttc ccaaccgcgc gcgagggctt
     7381 ccgcatcggt agtcagttta tctgcggctt ccagcaaaac gcgccctcgc tccagcaaca
     7441 tcctcccgac attggtgaat ttggtgcgat ggcccgagcg gtcaaacagc accacatcca
     7501 gctcttcttc cagtttttgc atggtgtagc taagtgcgga aggcacgcgt cccagctcat
     7561 ccgccgccgc cgcaaaactg ccccggcgat cgatcgcatc cataacccgt agtgcttcca
     7621 gcgttaatgc cctttctttg gccatttcgt tctcattcag gaaatttgaa cataccgggc
     7681 agaatatctg gctaacaatg tagcgtccag ccccttacca taaaaggaag taaagagagg
     7741 tcaagaatta tgattactac ccgaactgcc agacagtgtg gacaagcaga ctacggatgg
     7801 ttgcaggccc ggtatacttt ttcctttgga cactacttcg acccgaaatt gttaggctat
     7861 gcctccctgc gtgtgcttaa ccaggaagtg ctggccccag gtgccgcctt tcagccgcga
     7921 acctatccca aagtcgatat tttaaatgtg attctggatg gagaagcaga gtatcgcgac
     7981 agcgaaggca atcatgttca ggccagcgcc ggtgaggcgt tgctgctctc tacccagccg
     8041 ggtgtcagct acagcgaaca caatctcagc aaagacaaac cgttaacacg aatgcagctt
     8101 tggctggacg cctgcccgca gcgagagaat ccgctgattc aaaaactggc gcttaatatg
     8161 ggcaagcagc aattaatcgc ctcgccagag ggtacgatgg gaagcctgca attacgccag
     8221 caagtgtggc tgcaccatat cgtgctcgac aaaggcgaaa gtgcgaattt ccagttgcat
     8281 gggccacgcg cgtatttgca atcgattcac gggaaatttc atgcgcttac gcatcatgaa
     8341 gagaaagcgg cgctgacctg cggtgatggg gcgtttattc gtgacgaggc taacattacg
     8401 ctggttgccg attccccact gcgcgctttg ctgatagatt tgcctgtcta gttgttttta
     8461 cagggagatg atgatgagta aaaaatcggc caaaaagcgc cagccagtga agcccgtggt
     8521 ggcgaaagaa cctgctcgca ccgccaaaaa ttttggctat gaagagatgt tgagcgagct
     8581 ggaagccatc gtcgcggatg ctgaaacgcg tttagccgag gatgaagcta ccgcgtaaag
     8641 acaggttgcc ggatgcggcg aaaacgcctt atccggccta caaaatccaa caacattaac
     8701 gaattaaaca acccgtaggc cggataagat gcgccagcat cgcatccggc atcaggtaca
     8761 aaacacaata acatcaacaa gttgagcacc ccgtaggccg gataaggtgc gccagcatcg
     8821 catccggcac gatacccaaa acctggcgtt tatctggcct tgctcgccat aatctcgata
     8881 atctgccgat ccgtttgctg catcgaatgg cttgctaacg cacacaggtt ggcaatcgac
     8941 tgctcaacat catgcgccac gatcccttca ttgccggtca cggcggtatc atccagcgcc
     9001 attaacaccg ctttccacgc agccgaagca ctggtcgaaa ccttcatcgc gcagctgttc
     9061 gacgcaccat cgcaaatcat gccgctgaca tcgccgatca tactgctgat cgccatcgag
     9121 atggtttcat aacgcccatc caccagccat gccatcccgg cggcggcccc cattgctgcg
     9181 gtcgttgcgg cacacagcgc agacaaacgc ggtaactggt tatggatgta aattgcgctc
     9241 aaatgagaaa gcatcagcgc acgcgccagc cgttcatcat ccgctccgaa gtgttctgct
     9301 accaccacca caggcattgt tgcggtgatc ccctggttac ccgagccgga gttactcata
     9361 gccggaagcg tagcgccgcc catacgcgca tcggatgccg cgctggtacg aatcacaatg
     9421 gatgaagaga gatctttcgc cagcaaaccg cgctcgcact gtttttccag cgtcgcgcca
     9481 atatgcagcc cccacttacc gctcaaacct tcctgcgata acgcacaatt tagcttcgcg
     9541 gaatcgagaa taaagcggat cgccgcaaac gggacttcat tgacgaactt caggatctca
     9601 gccagcgtcg ttctggaaag caccgtcagc ggagactctt gctcgccctc tgccacacac
     9661 gcctgctggg taaacaccac accattgtgc gtctcaatat gcacaatgtt ggtatgcccg
     9721 ccgacgatgg tgacacacgc ccacttctca ccgttccaga ctttggcgcg tgagaagagg
     9781 atttcattgc aaggttcctg gatcttaacg gagactttcc ccgccgccag cagtgctttg
     9841 gcatcggcaa ttgcctgcgc agttgcgtct ttcagcactt ccagccctgc gttggcattt
     9901 ccacctaacg cccccagcgc cgccgcaatc ggcagcccca ccattcccgt gccgggaacg
     9961 gtgacgccca gaccgttctt catcagattt ggcgaaaccc aggcttctac acgttcaacc
    10021 ggaccttcca gttctgccgc agcaaccgcc gccgccagcg ccagtgaaat cggttcagta
    10081 catcccagcg ccggttttac ttcctcctga acggcgagga tgtaacgctg ccataacgga
    10141 tttaaagtcg aatcaaacat attaaaaacc ttaaaatttc aggtaaatca agaaaatgcc
    10201 aggaacggag aaacacaaag caacaaacca gtgacgataa tcaggtacag agacatccct
    10261 ttgtatttgt gcaatgccgg tactttgtaa accagccacg ccgggatcag gcaccctacc
    10321 atgccgaaaa tcgggctaca gatagaggtg aagctcaaca ccggtgcgtt cagtacgatg
    10381 gcgctccagg ccagcaaaat ggcgaaaatc atgatgccgc gctgaacaag attttcgtta
    10441 atcttctcgg caggcatctt gcgacgcagg atgttcatta cgatcccttg cgttgcttcg
    10501 cgaaagccta aatagacgcc aaagaatgca gtcatgacag caaagatatt gagaatgacg
    10561 ctgaccactt tcacccacgc tgcgccgtca ccgctaataa actgtgcggc aatcgccagc
    10621 gcggaaatat tctgctcata ggcttttacc gcttcgtcat gtcccatcgc cagcgtgaac
    10681 gacacggcgt agaaaaagac ggtgacaaac aaaatgccaa acgcgatatt cattgcccgc
    10741 aatgctttat gccgcgccac ttcaattgat ttttcccgcg agcgataaga gatcaccatc
    10801 gggcttaacg tctggataaa cagaatcgac gtcagggtaa acggcagcgt aataatggcg
    10861 tttttcacca gcagccccag cggcggtagc gaaccgacgt tatacagatg ccacattccc
    10921 accatcgaca cgcccagcgc cgcgaccacc agcagcttgg tcagcaccat gccggtcgaa
    10981 attttgaata acaatttctc gccgcgtgaa gagatcgcca ccagaatgca aatcagtacc
    11041 agaccataaa agggactgtc tgacagcaac ccttccgtca cgccgaaggt atgcagatag
    11101 gaagcactat cgttggtgat ggcggtggaa tagacgaaca tccaaatcac cagcattacg
    11161 aaatagagcg cacctaacag gatgccccag tttttaccta aataaccgct aatgacactc
    11221 gggtaatctt tacattctgg tgattctgcc agcgtattaa taaacaaccg ctgaaacaga
    11281 tacattgccg ggtaaccaat caccgatgag agcaaaaata cccacaatcc catcaaaccg
    11341 acctgcaccg ggagaaaaac aatccccgcg ccaatcgcca tcccgatact cataatcacc
    11401 cagccggtgt cagtactgtc gaatttgatc gcttctcgcc actcgctctc actcattcca
    11461 gcacgacccg ccggggtcga agcgtctgca atgacgcctt tattcgatgc aatttccata
    11521 atttctcgct caatattttg tagggcttat tatttttttc cgagccgcat caaggcgata
    11581 tgcggtctac gtttttgcag gcgagttatt agaataaaga aatgatacgc gcgaggtcag
    11641 agaaaatctt cacaatcaag actttgaaat aacggagatg gataagaatt ttctactaaa
    11701 ttaatcgcaa gagaatattc attctctatt tgcggcagat cacaaaaaaa aggtgcacat
    11761 ttgtgcaccc aaggatgaaa gctgacagca atgtcagccg cagaccactt taatggccag
    11821 tcctccgcgt gatgtttcgc ggtatttatc gttcatatct ttgccggttt catacatcgt
    11881 ctcgatcact ttatcgagtg aaacacgcgg tgccgaggtg cggcgcatcg ccatccgcgc
    11941 ggcgtttact gctttcacgg cattaatggc attacgttca atgcacggga tttgtacctg
    12001 tccggcaacc ggatcgcagg tcagcccaag gttatgctcc atcgcgattt ccgccgcatt
    12061 gcatacctgc gccggactgc cgcccagtag ttcagttaac cctgccgccg ccattgaaca
    12121 ggccacgcca atctccccct gacagccgac ctccgcgcca gagatggagg cgttcatttt
    12181 atacagcgcg ccaatagccc ccgctgccag aaaatagcgg gcaattgacc gctcgtttac
    12241 cggacgacgg aacttatcgt aataagccag cactgccgga ataatgccgc acgcaccgtt
    12301 agtcggtgcc gttaccacgc gcccgccagc tgcgttttct tcactaaccg ccagcgcgta
    12361 catgttgatc cagtcgatga cattcatcgg atcgttagag atgttatcgc tggaaaccag
    12421 ctgacgacgc agcgctacgg cacgacgcgg cacattgagc ggaccaggca gcacgccttc
    12481 agtgttcatc ccgcgttcaa taccgtcatg catcacttgc cagatacggg caaaccctgc
    12541 gtcaatttcc gctttgctgc gcagcgccag ctcgttgtgc atcatcaggc cagatataga
    12601 caggccgttg taatcacaca ttttcagcag ttcacctgct gagtggaaat cgtaaggtac
    12661 ggacgtttcg acatcgtgcg acaggccgaa gtgttcttct tcgacaataa acccgccgcc
    12721 gacagagtaa taggttttac ttaatagcgc ttcctgtccc ttccaggcag tgatccgcat
    12781 tccgttctca tggcgaggca acatttcggg atggaagata atgttctttg ctacaggaaa
    12841 atcaacaata tgcgcaccag atgccactgg cagccgcccg ctgcgcgtta ctaactctat
    12901 aaatgcaggg atctcatcaa tgacaacatc ctgcggactg tttcctgcca gtcccatgat
    12961 aatggcgaca tccgtggcat ggcctttgcc cgtcagtgac aacgacccat acagatcgac
    13021 cacaatatgg ctcgtcgcgg ttaataagcc gctactttcc agccgatcaa taaaactttt
    13081 tccggcattc attggcccca cggtatgcga actggaggga ccaatcccaa ttttgaaaat
    13141 atcgaatgca ctaatcatat ccacaccctc ggattgccgt tcagtgaagt ggagcggaac
    13201 gaccttgcga ccgtcccgct cacgaggctt tacgcactac gtactgcgat agcttcaatt
    13261 tccagcttca catctttcgg caaacgcgca acctgcacac agctccgggt cgggtaggtc
    13321 gcctgatgct catcgaagaa ctgcttatag acttcattga tggtggaaaa atcattcaga
    13381 tcggtgatga acacggtcat cttgatgata tcgcccacgc tcagcccggc agcaaccacg
    13441 atcgctttga cgttttcgag gcttaaacgc gcctgatctt gcacatcagc cgggatctca
    13501 ccggtctgcg ggcaaaccgg aatttgcccg gaggtgaaga ccatgctgcc taaatcaacg
    13561 ccctgaacat aagggccgat tgcgcctggg gcacgttgcg tttcgataat ctttttcata
    13621 catcctcctg cgtcagagcg cctgggtaaa agtacgtgaa ataacatcct gttgctgttc
    13681 acgggtcagt gcgttgaagc gcacggcgta gccagagaca cggattgtca ggttaggata
    13741 tttttccggg tgctcgatgg catccagcag catttcccga ttcattacgt tgacgttgag
    13801 gtgttgaccg ccttcgacat ccgcttcgtg gtggaaatac ccatccagca ggccgacaag
    13861 gttggtttta cgtactggat cttctttgcc cagcgccgca ggaacgattg agaaggtgta
    13921 cgagatccca tctttggcgt aggtgaacgg cagtttcgcc accgacgtca atgaggccac
    13981 ggcacctttg cggtcacgac catgcatcgg gttagcgccc ggcgcgaacg gtgttccggc
    14041 gcgacgaccg tccggcgtat taccggtttt ctggccgtac accacgttag aagtgatagt
    14101 cagaatcgac tgggtaggga cggcgttgcg ataggttggc agcgctttaa ttttcttcat
    14161 aaagcgttca accaggtcgc aggcaatgct gtctacgcgc tcgtcgttgt tgccgtactg
    14221 cggatattca ccgtcgattt caaagtccac cgccaggccg ttttcgtcac ggattggttt
    14281 cacgcgggca tatttgatgg cagacaggga gtccgtcgcc accgacaggc ccgcgatgcc
    14341 gcatgccata gtgcgataga catcacgatc gtgcagcgcc atcagcgaag cttcgtagct
    14401 gtacttgtcg tgcatgtagt gaatgatatt cagcgcgctg atgtactgca ccgccagcca
    14461 gtccatgaag tgatcgaggc tgtccatcac tttgtcgtaa tccagcacgt cgtccatcag
    14521 cggtgctgtt ttcggcccga cctgaatctt cagcttctcg tccaccccgc cgttaattgc
    14581 gtagagcagc gttttcgcca ggttagcgcg tgcaccaaag aactgcattt gcttaccaat
    14641 caccattggg ctgacgcagc aggcaatcgc gtaatcgtcg ctgttgaagt cagtacgcat
    14701 cagatcgtca ttttcatact gcaaggaaga ggtgacgatc gacacctgcg cggcatattt
    14761 tttgaaggca atcggtaatt cttccgacca aagaatggtc aggttaggtt ccggtgccgg
    14821 ccccatagtg tgcagggtgt gcaaatagcg gaaggagttt ttggtcacca gcgtacgacc
    14881 gtccagcccc atcccgccga tcacttccgt cgcccagatt gggtcgccgg agaacagcga
    14941 atcaaattcc ggtgtacgca ggaagcgtac catacggatc ttcatgatga agtgatcgat
    15001 cagttcctgt gcctgctgct cattgagtac gccagcttta aagtcgcgct caatgtagat
    15061 atcgaggaac gatgccgtgc ggcccagcga catcgcgccg ccattttgcg atttcactgc
    15121 cgccagataa gcgaagtaga gccactgcac cgcttcctgc gcattctgcg ccgggcgaga
    15181 gatatcaaag ccatatttcg ccgccatttc ctgaatctgc aacagcgcat gacgatgctc
    15241 tgccagctcc tcacgcagac ggatggtggc ttccagatcc tcgccttttt ccagacgaga
    15301 ctggagatcg gcaaattgca gttcgcgttc acgtaccaga taactgatgc catacagcgc
    15361 tacgcggcga tagtcaccga taatgcgccc acggccatag ccatctggta aaccggtcag
    15421 cacgccagat ttacggcagc gcagcatatc cggtgagtaa acatcaaata cgccctggtt
    15481 atgggtttta cgcagatcgg taaacagata ttcaaattca ctgtccattt cacggccata
    15541 ggcgtggaat gaacttttaa tcatattaat gccaccgaac gggtgtagcg cacgtttcaa
    15601 cggcgcatcc gtttgcaggc caacaatttt ttccagcggc tggttaatat atcccgcatc
    15661 atgagcggta attgtggtgg caatattggt atcgaaatca accggcgcgt gggttgcgtt
    15721 ttcgatacgg ataccttcca ttactttttc ccacaattcc gtggtggcag gcgtcgcttc
    15781 ggcgaggaaa gattcatcgc cttcatacgg tgtatagtta tgttgaataa aatcgcggac
    15841 attaatttcg tttttccagt ccgtaccttt aaagccaagc catgcgtcgg cgtacagctt
    15901 atcgctggta tcaatatcta ccttcatgaa aaataatctc tctacaatac ttcaactaaa
    15961 ttatgcaaat tctgcgggcg cgttaacttt gcctaaatga atggcatcca aagcaatcat
    16021 tttttcttcg ttagtcggaa taacagcgca aatgacacgc gcattttcac tggaaacaat
    16081 tcgctcacca caggagttag agcgattatt catttctgta tcaatctcta cgcctaatac
    16141 agccaaatgt tccatgacca gacgacgaat taagcttgaa ttctctccta ttccgccggt
    16201 gaatataatt ccatccaggc gacgtaatga agctgcgtgt ccggcaatat gacgggcaat
    16261 tcggtgaaca aaggttttaa ttgccagttg cgcgcgttcg tgaccttcat gccaggcttt
    16321 ttccagaaca cgtaaatccg aagaaagacc ggaaatacct aataatcccg actctttatt
    16381 cactacgcgt tccaggtcac ccaagctctg gttggtttgg ctggcgaccc aggacatcgc
    16441 accaaaatcg acatcgccac tgcgggtacc catcatcaag ccttccagcg gcgtcattcc
    16501 cattgaggtg tcaacactct gaccgttgcg aactgcgcag attgacgcgc cattgccaag
    16561 atgcgccaca accaggccgg agtcatcttc cgccagattc agcaaagaat gtgcgcgctg
    16621 ggaaacatag cggtgcgacg tgccgtggaa accataacgg cgtacaccta actcttcata
    16681 atatttccac ggcaggccgt ataaataagc ttccggagcc atcgtctggt ggaaactggt
    16741 atcaaatacc gccacctgag ttacgcccgg aaataattgc tgcgccgatt caataccact
    16801 taaattggcg taattatgca ggggtgccag tggagaaacg cgacggatat tatcaatgac
    16861 ctcatcggta ataatggcgg actcggtaaa aatactgccg ccgtgagcga tgcggtggcc
    16921 aattaaggcc acactgtcat ttaaattccg tttttccagt tcaaatgcaa ttgccttcaa
    16981 tgcaccttcg tagctgtggt gagccagcgg tgctggctct cccccattta cggataagaa
    17041 tgcattttcc gagttaatac cgtcggcaat acctgacatt aatacttcac agtcgctggc
    17101 atcgagcacg gaaaacttaa tcgaagacga accacagtta ataaccaaaa caaccggaaa
    17161 ttcattcatc tcttttctca tcctgagtta cggattaaaa cagtttgtat acgatgttca
    17221 ggatggtcag cagaccaatc acggtaacaa acacgttatc cagacgacca cggtatttcg
    17281 ccagagacgg cgctttacgg atggcataca tcggcaacag gcacagcagg gatgcgataa
    17341 tcggtgcgcc catggcttca atcaggtcaa ggatgttcgg gttggcgtag gcaacaaccc
    17401 aggtggagcc catgatgaag atcatgctga tagtgttcag tttacccagc gacactttag
    17461 ttttgtcgcc tttataacca aacttcagga ccaggccatt cagaccttcc agcgttccca
    17521 gatagtgacc gaagaaagat ttgaagatag ccacgagtgc gatgatggaa gccgcatatt
    17581 ccagtgtaat cgcgaacgtt gttttggtac cggtcatgga cgcaaagtgg ttagccagat
    17641 aagaaagcac tggaatattc tgcgctttgg cttccgccat gttggccgga gacagcgtaa
    17701 acaggcagct aaaggcaaag aacatcacca ctgcaaccat cagcatgctg gcacgagaaa
    17761 tgatttggga acatttacgt tcggtgaagt cgcgaccgaa gtctttctca tactcttcac
    17821 gtttagaaac cacgaaggaa gagacgattg gcgagaagtt aaaggagaaa accatgatgg
    17881 aaatccccag ccagacagtg atcaggatac cgtcatgacc ggttaacgac agcgaaccga
    17941 ggtcaacctg gtcgataact gcagagttcc agtaagggat cagcgacaga gaaatcagca
    18001 ccaggctggc gataaacggc cataccaggt agctcatcac tttaaccatc agatccttac
    18061 caaaccagat gacgaaagcc atcagcagca acaggaacag cgccacaaag ccgcgattca
    18121 gcggtgcaaa tccgagctgg ttttcccaga acgtcataaa ggtattggta atagtaacgc
    18181 cataaatcca cagcagtggg caaatcgcga agaagtacag gaacgtgata accacgccgc
    18241 cagttttacc aaaatgctct tccaccgttt ccgtaatgtt gccggaaggg ttagagccag
    18301 aaagacacag acgcgccagc gcccggtggc aataaaatgc gatggggtat gccaatacca
    18361 acatcagaag aatcgggatc agtccgccaa aacctgcgcg gatagggaag aacagcaccc
    18421 cggcgccgat tgccgtacca aacaagccta acgtccatgt ggtatctgat ttacgccagg
    18481 acgattgttt tgtctggctg gatacaatgc tatctgaagt actcatatcc tatcctcaac
    18541 gaattaatta agcgtcaacg aaaccggtga tttgagagac gcgagaaaga tcgatattgc
    18601 cgccggaaat aatactgacg gtttttctgt tttgaatata ttggtctaat ttaccgctta
    18661 ataatgcagc acatgccaga gcgcctgcgc cttcggtgac gactttattg cgctgaatta
    18721 aggcaatcat actgtttctg atttcgtctt cgctgaccag cacgatgtca tcgactaatt
    18781 cacgaacgat ttcgtaagtt aaattacccg ggcgggagac atcacaacca tccgccaggg
    18841 tgccggtagt tcggtgcgtg gttatttctc cggagtggaa agaagccgcc atgccgtgaa
    18901 cgttttcaga ctgtacgcca ataacacgaa tggtcgggtt aatagattta attgccaccg
    18961 caataccagc aattaaaccg ccaccaccaa ttggcacaat cacgttatcg acatcataga
    19021 gatcttccat aatttccaga ccaatcgttc cctggccagc aatcactttc ggatcatcgt
    19081 aaggtgggat aaaaatacgg ccttccattt cgacaatttc gctcacttta gcgatagtgt
    19141 cgttgaaatt atcaccatgc agaacgactt ctgcggagta gtcgcacgtt gccgctactt
    19201 tggattttgg cgcacctttt ggcatcacca ctttaccgtc gatacccagc atcgcgcagg
    19261 agagcgaaac cccttgcgca tggttgcccg cagaacaggc cactacacct ttacgttttt
    19321 ccgcatcagt cagtgaactt aatttattaa atgcgccacg aattttaaat gaacccgtac
    19381 gctgcatatt ttcaaacttc aggaatattt cacctttgca acgttcacta aaatagtttg
    19441 agcgaggcat gcctgtttta taaattcgcc cagccagtcg ttgtttcgct tcaataatat
    19501 catcaatagc aaccggcaga tcgtatgtaa tatgcattat aaaacctctt cgcctgaatt
    19561 aaatatgtag gtaaccgtaa ccgacacctg caaaacagac aggtggatta tttatgttta
    19621 aataaaaaca aataactaac caacttctat taattgcctt cgtctacacc cattataaga
    19681 tgaatactct ttggctaatt ccaccaaaac cgatgctgct tttttaatac gataattttt
    19741 cgaccatacc gcggcatatt gtgccacagg taatgtttct tcaaccggaa tagtaataaa
    19801 ttgattagaa ccaaaaggtg acgtcatatc acaaggaatt acagttaaga aatcagcatt
    19861 gagaacaaga ttataaattg tcacgactga gtcggtttta acgatgtttt caatactgat
    19921 gccatttctt tgtaacgtag taagcagttc gctgtagtac cccatattag tttgtggcaa
    19981 cacccactgt tcgttcttca acgactccag cgtggtggtg ccggtgcatg ttcgggactt
    20041 actggctacc agcacaaact cggactcgaa cagcggctca acatgtaaat cctgaagctt
    20101 catttctgca cttaacgtcc caatcgcaaa atccagtcga ccgtcgcgga ttgccggtaa
    20161 gaacgaagac agttgcgctt catacataga aacctgcgct ttcgggaaca cctctttgaa
    20221 cttgttgatc atccctgaca taaaagtaaa accaatcaat gaaggaaaac caaatgagac
    20281 ttccaccacc gcctcagaag acataccgct tatctcatta accatatttt tcatttcacg
    20341 ggtaatggat tcggaacgag agagtaacag ttgaccggct ggtgttaatg ttacaccggt
    20401 atttttccgc accactaatt ccacaccaaa ataatcttca atatcgttaa tgattttact
    20461 gacggccggt tgagttaacc ctaattcttt tgcagccgag ccgatagagc cacttctaat
    20521 gacttcctga aagactacca ggtgctgcgt tttcggaaga agaatagtgc tcataacgac
    20581 ctacgttaat tacctcattg acggcatgaa gtgtatcaaa atgaaatgaa caggatatgt
    20641 gcgaccactc acaaattaac tttcaatact ttccagggta tcgttattac aattaaatac
    20701 ctttaaatat caacaagtta aagtataaaa atcagcataa accctgattt ttgtcaaaaa
    20761 aaatgacggg gataaccata ttttatggcg ataacatcat tcgttatgtg gt
//
LOCUS       DAZGSP010000089        20565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZGSP010000089 DAZGSP010000000
VERSION     DAZGSP010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.089"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(79..1083)
                     /gene="argF"
                     /locus_tag="ACHK74_003643"
     CDS             complement(79..1083)
                     /gene="argF"
                     /locus_tag="ACHK74_003643"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFP0222391.1"
                     /translation="MSRFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1245..1661
                     /gene="rraB"
                     /locus_tag="ACHK74_003644"
     CDS             1245..1661
                     /gene="rraB"
                     /locus_tag="ACHK74_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFP0222392.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1707..2210)
                     /gene="yjgM"
                     /locus_tag="ACHK74_003645"
     CDS             complement(1707..2210)
                     /gene="yjgM"
                     /locus_tag="ACHK74_003645"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0222393.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYDGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            2403..3599
                     /gene="yjgN"
                     /locus_tag="ACHK74_003646"
     CDS             2403..3599
                     /gene="yjgN"
                     /locus_tag="ACHK74_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFP0222394.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIM
                     PSLPFL"
     gene            complement(3655..6510)
                     /gene="valS"
                     /locus_tag="ACHK74_003647"
     CDS             complement(3655..6510)
                     /gene="valS"
                     /locus_tag="ACHK74_003647"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFP0222395.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(6510..6953)
                     /gene="holC"
                     /locus_tag="ACHK74_003648"
     CDS             complement(6510..6953)
                     /gene="holC"
                     /locus_tag="ACHK74_003648"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFP0222396.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(7087..8598)
                     /gene="pepA"
                     /locus_tag="ACHK74_003649"
     CDS             complement(7087..8598)
                     /gene="pepA"
                     /locus_tag="ACHK74_003649"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFP0222397.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8752..8802
                     /gene="ytgA"
                     /locus_tag="ACHK74_003650"
     CDS             8752..8802
                     /gene="ytgA"
                     /locus_tag="ACHK74_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFP0222398.1"
                     /translation="MSIIILATISTTQENK"
     gene            8865..9965
                     /gene="lptF"
                     /locus_tag="ACHK74_003651"
     CDS             8865..9965
                     /gene="lptF"
                     /locus_tag="ACHK74_003651"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFP0222399.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            9965..11047
                     /gene="lptG"
                     /locus_tag="ACHK74_003652"
     CDS             9965..11047
                     /gene="lptG"
                     /locus_tag="ACHK74_003652"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFP0222400.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(11166..12668)
                     /gene="yjgR"
                     /locus_tag="ACHK74_003653"
     CDS             complement(11166..12668)
                     /gene="yjgR"
                     /locus_tag="ACHK74_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFP0222401.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAIIQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMISELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(12746..13744)
                     /gene="idnR"
                     /locus_tag="ACHK74_003654"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(12746..13744)
                     /gene="idnR"
                     /locus_tag="ACHK74_003654"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFP0222402.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYQGNTL"
     gene            complement(13811..15130)
                     /gene="idnT"
                     /locus_tag="ACHK74_003655"
     CDS             complement(13811..15130)
                     /gene="idnT"
                     /locus_tag="ACHK74_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFP0222403.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGHYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(15195..15959)
                     /gene="idnO"
                     /locus_tag="ACHK74_003656"
     CDS             complement(15195..15959)
                     /gene="idnO"
                     /locus_tag="ACHK74_003656"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFP0222404.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRAPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(15983..17014)
                     /gene="idnD"
                     /locus_tag="ACHK74_003657"
     CDS             complement(15983..17014)
                     /gene="idnD"
                     /locus_tag="ACHK74_003657"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFP0222405.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTEMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLRGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            17231..17794
                     /gene="idnK"
                     /locus_tag="ACHK74_003658"
     CDS             17231..17794
                     /gene="idnK"
                     /locus_tag="ACHK74_003658"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP0222406.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKHYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            complement(17798..18817)
                     /gene="ahr"
                     /locus_tag="ACHK74_003659"
     CDS             complement(17798..18817)
                     /gene="ahr"
                     /locus_tag="ACHK74_003659"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFP0222407.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRVDWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQELLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            19013..19097
                     /locus_tag="ACHK74_003660"
     tRNA            19013..19097
                     /locus_tag="ACHK74_003660"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19047..19049,aa:Leu,seq:caa)
     gene            19261..20526
                     /locus_tag="ACHK74_003661"
     CDS             19261..20526
                     /locus_tag="ACHK74_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0222408.1"
                     /translation="MKLSARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPTVTLAQARAKRDEAKKLLVQGIDPKAEQKEAQAENSGAYTFET
                     IAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRLLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKVRHYPALPSCRLPEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWNEFDFDKALWRVPAKREEIKGVRYSYR
                     GMKMKEEHIVPLSRQAMNLLGQLKQISGDKKLLFPGDHDATKVMSENTVNNALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHTERNNVRAAYIHTSEHLDERRL
                     MVQWWADYLDENKLRMITPFDYGKISNIK"
BASE COUNT         4990 a         5230 c         5095 g         5250 t
ORIGIN      
        1 taattgaact attactgcat aaaataatgt gatgccggga tgtttttgta tttcccggca
       61 tctttatagc gatatcaatt atttactgag cgtcgcgacc atcaccgctt tgatggtatg
      121 catgcggttt tccgcctgat caaaaacaat gctggcggca gattcgaaga cctcatcagt
      181 cacttccatt ccgccatgta ggccaaactc ttccgccatt ttcttaccaa gcgtcgtctg
      241 gtcgtcatga aacgcgggca ggcaatggag gaatttgacc tccgggttac cggtcaactg
      301 catcattttg ctgttcacct gataatcacg cagtaatgca atccgttctg cccatttctc
      361 ttttgcttcc cccatcgaca cccatacatc ggtatagata aagtcagcac cttcaactcc
      421 tttggctaca tcttccgtca gcgtaatatt cccgccattt tgctgtgcca gggcgcggca
      481 ttccgtaacc agcgcagctt ccggccagca tgcctgtggc gcgaccagac gcaaatccag
      541 accggtaagc gccgcagctt cgagcatcga attgcccatg ttgttacgtg cgtcacctgc
      601 atagaccagc gtcatttcgt tgaatgcttt accgggtaaa tgctcctgca tggtgaggag
      661 atccgccagc aactgcgtgg ggtgaaactc attggtcagg ccgttccata ccggcacgcc
      721 agcgtattcc gccagtgttt cgacaatctc ctgaccatag ccgcgatact gaataccgtc
      781 atacatgcga cccagcacgc gggcagtgtc tttaatcgac tctttatgac caatctgact
      841 gccgcttggg ccgagataag taacgcgagc accctggtca tatgcggcaa cttcgaaaga
      901 gcatcgggta cgggtcgagt ctttttcgaa gatgagcgcg atgtttttac cagtgagttt
      961 ggcttcttct ttgccgcttt tcttatcggc tttcagtttc gcggctaact gcagcaggct
     1021 gttgagttca gctggcgtga aatcgagtaa tttcaggaaa tgcttatgat aaaacctgga
     1081 catagatccc tcctgtggct aacgcctcaa tgaattaaaa ttcaatttat atggatgatt
     1141 attcatttgc aagtctaaag cataaatctt tgtcacaaag gtggaggcaa tgtcagtggt
     1201 gtgtgacaat aagagtatcg gcaggacatt aagaggaatg agccatggca aacccggaac
     1261 aactggaaga acagcgtgaa gaaacacgtt tgattattga agaattactg gaagatggca
     1321 gcgatccgga cgcgctgtac accatcgaac accatctttc cgcagacgat ctggaaaccc
     1381 tggaaaaagc agcagttgaa gcgtttaaac tcggttacga agtgaccgat ccagaagagc
     1441 tggaagttga agatggtgat atcgtgattt gctgcgacat cctcagcgag tgcgcgttga
     1501 atgccgatct gatcgatgcc caggttgaac aactgatgac gctggcggag aaatttgacg
     1561 ttgagtacga cggttggggc acttactttg aagatcctaa cggcgaagac ggcgacgatg
     1621 aagattttgt cgatgaagac gatgacgggg ttcgccacta attaattgat taaaatgccc
     1681 gatagcacac actagcgggc atttttttag agttcgcgta gcatccgcac ttcacaatct
     1741 acatggcccg tgcagccaag tgcatagtcg atatgttgaa aacctaaatg ttcatacaaa
     1801 ccaatcgctt ctttcaaaaa agcggtcgtt tccagatagc agcgtttgaa gcccatctct
     1861 cgtgcctgct ccatcgccat taaggccagt tttttcgcca gccctttacc gcgaatagcc
     1921 ggaagaaaat acatcttctg cagttcgcaa atgtccgact cactcccggt taatggcgca
     1981 atcccaccgc cgccgaccac ttcaccatcg tactcaacca cccaatatgc atgacccggc
     2041 tgactataca cttgatacaa ctcgtccagg ttcggatcgg cgacggtgta gcctttatca
     2101 gcggtaagac cgtattcggc ggatacctga cgaatgacgc gggcgatggc gggattatcc
     2161 tgctgcgtca ggcgacgcat tacaggtgat tgcggcgcaa tgttattcat agtttaaccc
     2221 aataaaaata aagtaattat acatttgttt aataccactc tcttcttacg tgcgcaagcg
     2281 acttataaag taataaagtt tttgccagcc tattactgat aagagtattg taacgtggca
     2341 gaacattttg ctgtctgtat atataaatta tatcaatgga ttttttaatt cagggaattt
     2401 ttatggctca agttattaat gaaatggatg ttccgtccca ttcgtttgtt tttcatggta
     2461 caggtgagag atattttctt atttgtgtgg tgaatgtgtt gttaacgatt ataacgctag
     2521 gtatctattt gccatgggca ttaatgaaat gtaagcgtta tctctatgct aatatggaag
     2581 ttaacggaca acgattttct tatggaatta ctggtgggaa tgtttttgtt agttgtcttg
     2641 tttttgtttt ttgctatttc gcaatcttaa tgacagtgtc agcagatatg ccacttgttg
     2701 gctgtgtttt gactttgtta ctgttggttt tgcttatatt tatggcagca aaaggactgc
     2761 gttatcaggc cttgatgacc agtctcaacg gcgtaagatt tagttttaat tgctctatga
     2821 aagggttctg gtgggtaacc tttttcttgc cgattttaat ggccattggg atggggactg
     2881 ttttctttat ctcgacaaag atgctacatg ccaatagttc aagtagtgtt attatatctg
     2941 tggttctgat ggcaatagtt ggtattgttt ccattggtat ttttaatggt actttatata
     3001 gcctggtaat gagttttctc tggagcaata ccagtttcgg tatacatcgt ttcaaggtga
     3061 aattagatac tacgtattgt ataaaatatg ccattctcgc atttttagct ttattacctt
     3121 ttctcgctgt tgctggttat attatcttcg atcaaatatt aaatgcgtat gatagttctg
     3181 tgtatgcaaa tgatgatatt gagaatttac agcaatttat ggaaatgcaa cgtaaaatga
     3241 taatcgcgca gttaatctat tattttggga ttgctgttag caccagttat ttaacggtgt
     3301 cgttgcgaaa tcattttatg agcaacctgt cactgaatga tgggcgtatt cgttttcgct
     3361 caactttaac gtaccacggt atgctttatc gcatgtgtgc gttggtggtg atatccggga
     3421 ttacgggcgg tctggcttat ccactgctga aaatatggat gattgactgg caggcaaaaa
     3481 atacgtattt gctgggcgat ttggatgacc ttcctttaat caataaagaa gaacaaccag
     3541 ataaaggctt cttagccagg atttcacggg gaattatgcc ttctttacca tttctgtaag
     3601 agataaaaaa ggccggagca tgctccggcc ttcgttttca ttactgtgtt ttgattacag
     3661 cgcggcaata acagcctgct gttcaatcag cttcgctttc gcttctgcat agccttccag
     3721 cttctcacgc tctttcgcga tgaccgcttc cggtgcgcgg gcgacaaagc cttcattcgc
     3781 cagcttgttc tcgatacggc tgatttcgcc ttcgatcttc gccacttctt tcgccagacg
     3841 cgccagctca tcttctttgt tgatgaggcc agccatcggg atcagcagct ctgcgccgtc
     3901 gatgatctta gtaacggaaa ccggaccttt gtcatcggca ggcagcacgg tgatgctttc
     3961 cagacgcgcc agggtttgca ggaagccacg gttttcattt acgcgacgtt ctgcatccgc
     4021 gctgcaacca cgcagtagca gctccagcgg tttgcccggc gcgatgttca tttctgcacg
     4081 gatgttacgt accgcaacga tcgcctgctt cagccactcg gtgtcggcca gtgcggcttc
     4141 atcaacctga gatgcatcgt actgcgggaa cggctgcagc atgatggtgt cggcagtgat
     4201 accgcaaagt actttcacac gctgccagat ggtttcggtg atgaacggaa tgatcggatg
     4261 cgcgaggcgc agcagacctt ccagtacagt caccagcgta tggcgagtac cgcgcagttc
     4321 tgcttcggtg ccaccgttca ttaccggctt ggtcagctcg agataccagt cacagaactg
     4381 gttccaggtg aactcataca gaatgcctgc ggcgatatcg aagcggaagc tgtccagcgc
     4441 ttcgcggtac gctttgatgg tctggttgaa ctccgccaga atccagcggt ccgccagcga
     4501 cagcgtcatt tcgccgccgt tgaagccgca atcctgacct tctgtgttca tcagcacaaa
     4561 gcggctggcg ttccacagct tgttacagaa gttacggtaa ccttccaggc gcttcatatc
     4621 ccagttgatg tcacgaccgg tagacgccag tgccgccagg gtgaagcgca gcgcgtcagt
     4681 accgtgcggt tcaataccgt tcgggaactg cttctcggtg cgcttacgga ttttgtccgc
     4741 cagctgtggc tgcatcatat tgccggtgcg tttttccagc agttctggca gcgaaatacc
     4801 gtcaaccata tccagtgggt cgataacgtt acccttggat ttggacatct tctggccttc
     4861 gtcgtcacga atcagaccgg tcatgtaaac agtgtggaac ggcacctgcg gtttgccatt
     4921 ttcatctttg atgaagtgca tggtcatcat gatcatgcgg gcaatccaga agaagatgat
     4981 gtcgaaacca gataccatca cgctggttgg gtggaactga cgcagggcgt cggtgttttc
     5041 cggccagcca agggtagaga aggtccacag cgcagaagag aaccaggtgt cgagaacgtc
     5101 ttcgtcctga cgcaggacaa catcagcacc gaggttattt tctttacgca cttcgtcttc
     5161 gttgcggcca acataaacgt tacccgcttc gtcataccat gccgggatac ggtgacccca
     5221 ccacaactga cgagagatac accagtcctg aatatcgcgc atccaggaga agtacatgtt
     5281 ttcgtactgc ttcggtacga actgaatgtc gccgttctca accgcttcaa ctgccggttt
     5341 cgccaggaca tcggcacgca cgtaccactg gtcggtcagc atcggttcga tgaccacgcc
     5401 accacggtcg ccataaggaa cggtcaggtc atgtggttta atttcttcca gcaggccgag
     5461 tgcgtcaacc gcagcaacga ccgctttacg tgcagcaaaa cgctccagtt tctggaattc
     5521 tgcggggatc tcgctggaat aaacgtcaga ttcgttacct ttggtatcga acacctgggc
     5581 actttcacgg atatcgccgt caaaggtcag gatgttgatc atcggcagcg cgtgacgttt
     5641 acccacttca tagtcgttaa agtcgtgcgc cggagtgatt ttcacgcagc cggtgccttt
     5701 ttccatgtcg gcgtgttcgt cgccaacgat cggaatacga cggttaacca gcggcagaat
     5761 gacatatttg ccaatcagat ctttgtaacg cggatcttcc gggttaacgg ctacgccagt
     5821 atcgcccagc agggtttccg gacgggtagt cgcaaccacc agataatctt taccgtctgc
     5881 ggttttcgcg ccgtcagcca gcggatagcg gatgtgccac atcgaacctt tcgattcgcg
     5941 gttttccact tccaggtcag agatagcggt gcgcagtttc gggtcccagt ttaccaggcg
     6001 cttgccacgg taaatcaggt cttctttata cagacgaacg aaaacttctt tcaccgcatt
     6061 ggacaggcct tcgtccatgg tgaagcgttc acgctcccag tccacggagt tgccgagacg
     6121 gcgcatctga cgggtaatgg tgccgccgga ttccgctttc cattcccaga ttttgtcgat
     6181 gaaagcttcg cggccgtagt cgtgacgggt tttaccttct tctgcggcaa tcttgcgctc
     6241 aacgaccatc tgggtagcga tcccggcgtg gtcagtaccg acctgccaca gggtgttttt
     6301 gccctgcatg cgctgatagc ggatcatggt atccatgatg gtttgctgga aggcgtgacc
     6361 catatgcaaa ctgccggtga cgttcggcgg cgggatcatg atgcagaaac tttcctggct
     6421 ttcatcgcca ttaggcttaa agtagccctg cttttcccag tgctcgtaaa gcggctgttc
     6481 gatatcttgt gggttatatg tcttttccat tatttccagg ttgccgtatt caggttgaaa
     6541 ccagccacgc ggtaggcttt atagcgttcg cgcgccagtt gtttcagaga atcttcataa
     6601 ggaacgaagt ctaccacttc tgtgaaagcg gtggcaaaat ctgcaaagct tgttcgcaga
     6661 ctaatcaata tatcgcgcgg gctgctgctg cgtttttgcg gccaggcgat ctccaccggc
     6721 gcaccgccgc gcggtccttc tcccgctaaa ttatgcggaa caaagctttc tgccggacgc
     6781 gcccacaggg cttcatccag ccggtaagcc tgcttttcat cttcacaggc gatgagcacg
     6841 cgcttaccgc tgcgccaacg ttctgcggca atttcacaca ccagttgctc aacggcgctt
     6901 aagccatcga cggtggtgtc attgtccaga aggtagaacg tcgcgttttt cataaatggg
     6961 gcttcttgtg gtggatttaa atattgctgc attcctcacc ctaaaagatg agcaatgaat
     7021 ttctattgcg ctcgatcggt tccctctccc ttagggagag ggttagggtg agggtaaaac
     7081 gcaaacttac tcttcgccgt taaacccagc gcggtttaac aggaactgtg ccagcaacgc
     7141 taccggacga ccggtggcgc cttttgcttt accagaacgc caggcggtac cggcgatatc
     7201 caggtgcgcc cagttgtact tacgggtaaa gcgtgacagg aagcaacctg cggtaatcgc
     7261 cccaccagga cgaccgccaa tgttcgccat atcggcaaaa ttggactcca gttgttcctg
     7321 atactcgtca cccagcggta agcgccatgc gcggtcaccg gattgttcag acgcggcaat
     7381 cagttcatgg gccagcggat tatggttcgc catcagacca gtaatatgat gacccagcgc
     7441 gatcacgcag gcaccggtca gcgtcgccac gtcaatcacc gcttccggct caaaacgctc
     7501 aacgtaagtt aacacgtcgc acagtaccag gcggccttca gcgtcggtgt tcagcacttc
     7561 aacggtttga ccggacatgg tggttaacac atcgcccgga cgataggctc gtccgccagg
     7621 catgttttcg cagcctgcca acacgccgat aacgttaatc ggcagttgta gctccgcgac
     7681 catccgcatc acgccgtaaa ccgctgccgc accgcacata tcgtacttca tctcatccat
     7741 gccttctgaa ggcttgatcg agataccgcc ggagtcgaag gttaaacctt tacccaccag
     7801 cacgattggg cgtgcatctt ccgacgcgtt gcctttgtac tcaatcaccg acatcagcga
     7861 ttcgttttgc gaaccctgac cgaccgccag ataggaatgc atccccagct ctttcatctg
     7921 ctgttcgccg ataacgcggg tgatgacatt cttgctgtag ctgtcagcca gctggcgcgc
     7981 ttgtgaagcg aggtaagcgg cgttacagat attcggcggc atattgccga gatcttttgc
     8041 tgctttaatc ccggcggcaa tcgccagacc gtgctggatc gcgcgctcac cgctggtcag
     8101 ttcacggcgg gtcggcacgt tgaacaccat tttacgcagc ggacgacgcg gttcgctctt
     8161 gttcgttttc agctgatcga aactgtagag cgtctctttt gctgtctcga cagcctgacg
     8221 cactttccag tagttgttac ggcctttaac gtgcagttca gtcagaaagc agaccgcttc
     8281 cattgagcca gtatcattca gcgtattaat ggttttctga ataacctgct tgtactgacg
     8341 ctcatccagc tcacgttctt tgccgcaacc aataaggaga attcgctcgg aaagtacatt
     8401 cggaacatgg tgcagcaaca atgtctgccc cggttttcct tccagttcgc cccgacgtag
     8461 cagggcgctg atgtacccat cgctgatttt atcgagctgt tctgcaatcg gagaaaggcg
     8521 acgtggttcg aagacgccca cgacgatgca ggcactccgc tgtttctccg ggctaccgct
     8581 ttttacacta aactccatgc actacgctcc tgaatcttaa agacaacggc ggtggctaca
     8641 gatagaattg caagctttcg taactcatgt ccgctgttgc gatgacttcg tgttaatctt
     8701 aacgttatta cggcattggc acgtcagaac aaaatctgag aggtgaatcc gttgagtata
     8761 attatcttag cgacgatttc gacgactcaa gagaataaat gacgtttaag ccatgaaaca
     8821 agctaaaatc ctgcaaaaga cgagttttta cgggcgtatt taaagtgata atcataagat
     8881 atctggtgcg ggagacgctc aaaagccagc tggcgatact cttcatcttg cttttgatct
     8941 tcttctgtca aaagttagtg aggatcctcg gcgcagcggt tgacggcgat attccggcga
     9001 atctggtgct ctcccttctc gggttgggcg tgccggaaat ggcgcagctt atcctgccat
     9061 taagcctgtt cctcgggctg ctgatgacgc tgggcaaact gtataccgaa agtgaaatta
     9121 cggtaatgca tgcctgcggc ctgagcaaag cggttctggt gaaagcggca atgatccttg
     9181 cggtattcac ggcaatcgtc gcggcggtta acgtgatgtg ggcgggaccg tggtcatcgc
     9241 gtcatcagga tgaagtgtta gcagaagcga aagcgaaccc tggcatggcg gcgctggcgc
     9301 aagggcaatt ccagcaagcg actaatggca gctcggtgct gttcatcgag agcgttgacg
     9361 gcagcgattt caaagatgtg ttcctcgcgc aaattcgacc aaaaggtaat gcacgtcctt
     9421 ctgtggtggt ggccgattcc ggacatttaa cccagctgcg cgacggctcc caggtcgtca
     9481 ctctcaacca gggaacgcgc ttcgaaggca ctgcactgtt acgtgatttc cgcattacgg
     9541 atttccagga ttatcaggcg atcattggtc accaggcggt ggcgctcgac ccgaacgata
     9601 ccgaccagat ggacatgcgc acattgtgga acactgacac cgatcgtgct cgcgcagaac
     9661 tgaactggcg tatcacgttg gtgttcaccg tgtttatgat ggcacttatg gtcgtaccgc
     9721 tgagcgtggt taacccgcgt caggggcgcg tactgtcgat gctgccagcc atgctgctgt
     9781 atctactttt cttcctgatc cagacctccc tgaaatcgaa tggcggtaaa ggtaagctag
     9841 acccgacgct gtggatgtgg accgttaacc tgatttatct ggctttggcg attgttctca
     9901 acctttggga caccgtgccg gtccgccgcc tgcgcgccag tttttcgcgt aaaggagcgg
     9961 tgtgatgcaa ccttttggcg tacttgaccg ctatatcggt aaaactattt tcaccaccat
    10021 catgatgacg ctgttcatgc tggtgtcgct gtcgggcatt atcaagtttg tcgatcagct
    10081 gaaaaaagcc gggcagggga gttacgacgc gttaggcgca ggaatgtata ccttgctgag
    10141 cgtgccgaaa gatgtgcaaa tcttcttccc gatggcggct ctgcttgggg cgttgcttgg
    10201 tcttgggatg ctggcgcagc gcagcgaact ggtggtgatg caggcttctg gttttacccg
    10261 tatgcaggtg gcgctgtcgg tgatgaaaac cgccattccg ctggtcttgc tgacgatggc
    10321 gattggcgaa tgggtcgcgc cgcagggcga gcagatggcg cgtaactacc gtgcgcaggc
    10381 gatgtacggc ggctcgttgc tctctaccca gcaaggctta tgggcgaaag atggcaacaa
    10441 cttcgtctac attgagcggg ttaaaggtga cgaagagtta ggtggcatca gcatttatgc
    10501 ctttaacgag aatcgtcgtc tgcaatccgt acgctatgcc gctactgcga agtttgaccc
    10561 ggaacataaa gtctggcgtc tgtcgcaggt tgatgaatct gatctgacca atccgaaaca
    10621 gattaccggt tcgcagacgg tgagcggcac ctggaaaacc aacctcacgc cagacaaact
    10681 gggcgtggtg gcgctggacc cggatgcact ctcaatcagc ggtttgcaca actacgtgaa
    10741 gtatctgaag tcgagcggtc aggacgccgg acgttatcag ctcaacatgt ggagcaaaat
    10801 cttccagccg ctatccgtgg cggtgatgat gctgatggcg ctgtcgttca tctttggccc
    10861 actgcgtagc gtaccgatgg gcgtgcgtgt ggtcaccggt atcagcttcg gttttgtctt
    10921 ctacgtactg gaccagatct tcggcccgtt gacgttggtt tatggcatcc cgccgatcat
    10981 cggcgcactg ttgccaagcg ccagcttctt cttaatcagc ctgtggctgt taatgagaaa
    11041 atcgtaacca agcaagggag cgaaagctcc ctttcacatt gttgataaag tcaaatacga
    11101 gttcgatcgg tcccctcgcc cctttgggga gagggttagg gtgaggggga cagatcgcgc
    11161 ctgacttacc ttcttctccc ccccagcaaa ctccccaaca ttccacgcac aatctgattc
    11221 gtcacctggc gagcggcgct tttggccatt gtttgcacca caccatcttt cttcccgccg
    11281 cgtggtccgg tagtgccgaa caaaatatcc ttcaatccac caagaatgcc gtcatccacc
    11341 gctacctctt tccctttcgc ggggggatta ttttgctgct cggtactggc ctgaaagcct
    11401 ttttgcaaca tctcataggc ggattcccgg tccacctcat cctcatattt gccatacacc
    11461 ggagagtgat taatcaagcc attacgctca tcttccgtca ccggccccat ccgcgaacaa
    11521 ggcgcgatca ccatcgcacg ctccaccaca gaagggcttc ctttcgcatc cagaaaagag
    11581 atcaacgcct cgccggtgcc cagttcctga atcgcctttt cggtatcaaa cgccggattg
    11641 gcccgcatgg tttgcgccgc agcttttacc gctttctgat ctttgggcgt aaaagcccgc
    11701 aaagcgtgct gaacgcgatt accgagctgg ccgagcacat tatccggaat atcagacggg
    11761 ttttgcgaaa cgaaccagac gcccacgcct tttgagcgaa taagccttat cacctgctca
    11821 atcttatcca gcagtacctg cggtgcatcg ttaaacagca gatgtgcttc gtcgaagaaa
    11881 aacaccagtt ttggcttctc cagatcgcct gcttccggca attgttcata caactctgaa
    11941 atcatccaca gcaggctggc ggcgtacagt ttcggcatct gataaagttt ctcggcgctg
    12001 aggatattga taacgccttt accgttggca tcggtgcgca tccagtcttt gatatccagc
    12061 atcggctcac caaagaagtg tgctgcgcct tgctgttcca gcgacaacag cccgcgctgg
    12121 atggcaccaa ccgatgcgct gctgatattt ccgtactgat tctggaaaga tttggcgtta
    12181 tcgccgatgt actggataat cgcccgcaaa tctttaaagt cgagcagtaa cagcccctga
    12241 tcgtcagcaa tgcggaagat gatattcagc acgccagatt gcacatcgtt gagattcaac
    12301 agccgcgcca gcaacagcgg ccccagatcc gaaaccgtcg cccgcaccgg atggcccttc
    12361 tcgccaaaga tatcccacac caccaccgga ttggcatgcg gttgccagtc gttgacaccg
    12421 atatttttca accgagcgag cagtttttcc gatgccgttc ctgcctgcgc gatgccggtc
    12481 agatcgcctt tcacatcggc cataaacacc ggaacaccga tttccgatag agactccgcc
    12541 agtttttgca gcgtgacagt tttacccgtc cccgttgcgc cagtaatcag cccgtgacgg
    12601 ttagccattc ccggcagtaa aaacagttct gtgtccggcg tgcgggcaat taacaggggt
    12661 tcgctcatga gatatcctcc agtttcctgc ctggagtata ggcaacgtcc ggcaaagaga
    12721 atacgtgtaa tgaagagaaa ataacctaaa gcgtgttgcc ttgataaatc tgatacccga
    12781 ggtcaacagt gttgtggttg tgatcgttat ttttaatttt gctcagcaac atttgggcgg
    12841 ccatccgacc aatatcaaaa cggggagtaa ttacgctggc gaggcttggg atcatttgcc
    12901 tgccaatctc cagcccatga aaacccgcaa tggaaatttg ttctggcacg gcgaggtttc
    12961 gctcgcggca cagcagaagc gcacccatcg caatatcgtc attggtgcaa aatacaccgt
    13021 ctaaatccgg gtttgcgcta agggcatcac gcattaactg cattcctaaa tgaatggatg
    13081 agatggcgcg tggattcatg cgtaatgggg aaagattatg cagcatcatc gcatcgcaat
    13141 acccctgata acgctgttca tcgcgggtgt catctttgga accgagatac agaattttgt
    13201 gcctgactcg cttctccagc atggtacaca ccatgtcaaa agcggcctgc cgattatcaa
    13261 aaccgacctc catatccagc cgttctccct gtacatccat caattccacg accggaatgg
    13321 tggctgaacg cagaaatttc accgtcctga tggtgtgata tttttccgaa agtataatcc
    13381 cgtcaatgtt ataagagagc aggttaatca ccgactcttc ttcggaatcg cgatcgtaat
    13441 tgtaattagc aataagggtc tgataattat gctcagaggt gacggattct attcccgcga
    13501 gaatgtcagc gaagagttga ttttgaaatg acggaatcaa gataccaagg gtataacttt
    13561 gtgcattcaa caacatgccg ggagcgcgat tagggatata attgatctct tccataattt
    13621 tggcgatgcg ctcgcctgtt tcctttgcca cctttttcgg cgagcggata taacgactca
    13681 cggtcatttt tgttacgcca gccagcgtag cgatatcctg taaagaaatt ctgtgattgc
    13741 gcataacttc tcccaacgtc gggggaatct tcaatcttcc cccgaaacta catcacaata
    13801 ttttattctt tcagtgcaat acggcgttaa tggctaacac gcccagcaaa cccataatag
    13861 aaataagggt ttccatcacc gtccaggtac gcaacgtttc accaaccgtc agattaaaat
    13921 accctttaaa tagccagaag ccagggtcgt ttacgtgtga cgcgatcacg ctgcccgcac
    13981 cggttgccag taccattaaa gcgggatcgg catgggtaac gttgataatc ggcaacacca
    14041 cacccgcggt ggtaatggcc gcgacggtgg cagagcccag agcgatacgc aacagcgccg
    14101 caacagtcca gcacatcaat aacggcgaaa gcgtagttcc ggtcattaag tgtgaaatat
    14161 agtgcccgac accgctatct actaatacct gcttaaacgc gccgccgcca gcgataataa
    14221 acacaatcat cgcgatagcg cctatagaat ccccaatgat atccatgatt tgctcgatgg
    14281 tgcgtccatt acgtcggccc agtgtgaaaa tcgcaataac aatggcaata aacagcgcaa
    14341 cggcagggtt accgacaaat tcaaagaaga ggcgcacggt gttagttttc ggtaacgtaa
    14401 tttcacaaac ggcggcgata gccatcagga tgaccgggat cacggcagcg aaaatactgt
    14461 tccagaagga gggcatctcc tcttcgctaa acagatgagg attaaataag ccttccggtg
    14521 gtgctttctc aaagcgagtt agcagtttag aaaacagcgg tcctgcgaca ataactgtcg
    14581 gaatggtaat gataaatcca tacagtaaag tcgttccgag attagcctca aagatagtcg
    14641 cgatggcagt aggccctgga tgtggcggca gaaaacagtg ggttacagag agcgctgcta
    14701 ccattggtac gccaacatac aacaggggta atcctgatga tgctacgatg gtaaatacca
    14761 acggcaacag caggacaaaa cccacttcaa aaaacatggc gaggcccaca accagaccgg
    14821 tgatcactag cgcccattgc acgcgttttt taccaaaagt agcaatcagc gtagtggcga
    14881 tacgttgtgc cgcacccgta tcagaaatca gcttgcctaa catggccccg aaaccgagaa
    14941 tcattgccag cccgccgagc gtgctgccga taccattttg tatagagtgc aggacggcct
    15001 gtgcatccat cccttcggca aatccgacga cggcagctac cagaacgagg gcaataaagc
    15061 cgttaacttt aaagccgatc atcaggataa gaagcagcgc gacgcctgcc gcaataatga
    15121 ttaatggcat agtctttatc tcttattagt gccagaaaag cagcgctttc gcgctgcacg
    15181 tagggtagta ggtgttaaac agccactaac atgccgccat caacaaacaa caggtggccg
    15241 tttacgaaat cagaggcttt tgaagaaagg aacaccgcag caccaatcag ttcctgcgga
    15301 tctccccagc gtgcggcggg ggcccgtttg cacaaccagg cggtgaaggc ttcgtcctca
    15361 accagtgctt tagtcatttc tgttttgaaa tagcccggcg caataccgtt gacctgaata
    15421 ttgtggcgcg ccagctcgac gcacatgccg cgggtgagca tttttaccgc ccctttcgat
    15481 gcggcataag gggtgatggt gtcacgtccc agttcgcttt gcatcgagca aatattaata
    15541 actttacctg ccttgcgttc aaccatgtga cgagttaccg cttgcgatac caggaacacg
    15601 gcggtctggt ttactgcgat cacatcattc cactcttgtt cagggaactc agtaaaagga
    15661 tgacggcgct ggataccggc gttattcacc agcacatcaa tggggccgat gtccttttcg
    15721 atatgttcaa cggcggcatc aatttcatgt ttatgagtaa cattaaaagg tgcggcaacg
    15781 gcctgaatac cctcctggtg gagtttttct acagcaagtt ctgcgcgttc ggcagtaata
    15841 tcattaataa ttatttgtgc gccatattta cccaggccgg ttgccagtaa aaagccaatg
    15901 ccctgtgctg aaccggtaat caagatattt tttcctgcca gtgaaaatag atcgttcatt
    15961 gtattattcc tttatttatc tcttagaaaa caagctggac ttttgctgcc tgggttttat
    16021 caccggcgaa acgtagcgcc tcttccaggt cagtgaaggg atattcagca ctcagtaaag
    16081 gcagtggatt gataacgcca ttcgccagcc atgacactgc ggtattaaat tcgctggtaa
    16141 aacggaaaga gcctctgagt gaaatctcct taccaatcaa cgtcatcatt gggaattctg
    16201 ccatcgcgcc tcccatacct acctgcacca ttacgccgcg tgcacgagtg acctccagac
    16261 aggtattcac tgatgaagga tgaccggaca cttcaaagct gacatcgaaa tagccttttt
    16321 ccgctttcca gtgatccatg tcgtcgtttt gtgggtttac gagcacatcc gcccccatct
    16381 ctttgcccag cgaaagggaa cggggactca catcagcaca gacaatttcc gcggccccca
    16441 gtgttttcac tgcactgaca atcaggcagc caatgggtcc aacaccggaa ataaatactc
    16501 gcttgccctg taactcgccg gcctgatgtg cggcatgaat cgcgacggct aaaggttcgg
    16561 caaaagccat aaccttttcg tcagctttgg ccggataagg gacacattgc gacgtttcga
    16621 ccattttata acgggtaaaa ccaccatcaa catgagggaa atacatggca ctgccaaaaa
    16681 aacgcatctc tgtacactga ttctcgttat gttcaatgca gtatttgcag tgaccgcacg
    16741 gtttagacgg attaatggct accgtttgcc cttcatgtaa ttctgatgag tcgctatgaa
    16801 taactttacc gataacttca tgacctaaca ccatcggtgc ctttatcatg aaattaccta
    16861 cttttccttc ctgataataa tgtaaatcgg aaccgcaaat tccacctcgg gttatttgta
    16921 ctaatgttcc attattattc caatctatcg tctgctcggt aacggcaaca gttttcttgc
    16981 ccgcaacaac gcaggactgt gttttcactt gcataaattc ctcactggtc aggtagacac
    17041 ctcggaagca tttaagcggt tttaattgtc attatttgtg atgaagatca cgtcagaaaa
    17101 ttgttacatt actatgttac gcataacgtg atgtgccttg taattcttat cagcagaaat
    17161 aaaaaaacgt gaaattatta tgccaccagg cgtagtatcg cagcaggtaa gatgattcag
    17221 gagattttaa atggcgggtg aaagctttat tttgatgggc gtttcaggga gtggtaaaac
    17281 attaattggt agcaaggttg ccgcgttatt atctgctaaa tttattgatg gtgacgatct
    17341 tcatccagcc aaaaatatag ataaaatgtc gcagggtatt ccattatctg atgaagatcg
    17401 acttccctgg ctggaacgct taaatgatgc ttcatacagt ctttataaaa agaatgaaac
    17461 aggatttatt gtctgttcat cattaaaaaa acattatcgt gatattttac gcaagggtag
    17521 cccccatgtt catttcctct ggttagatgg cgactatgaa actatcctcg cgcgaatgca
    17581 gcgtcgggct gggcatttta tgccggtagc gttactaaaa agtcagtttg aggcgctgga
    17641 gcgtccacaa gcagatgaac aggatattgt gcgcattgat atcaaccatg atattgccaa
    17701 tgtcaccgaa caatgtcggc aggctgtgct ggcgatacga caaaacagaa tatgtgcgaa
    17761 agagggcagc gcctcagatc agcgctgcga atgattttca aaaatcggct ttcaacacca
    17821 cgcggtaacg cgccttaccg tcgcgcacat gctggatggc gtcgttaatt ttcgacatcg
    17881 ggaacagttc ggttgtcggc gcaaccttgc tgcgggcggc aaagcgcatc agcttacgca
    17941 gttcataagg cgtgccggta gcagagccag agacgctgcg atcgcccgca attaacgtaa
    18001 aggccggaac agacagcggc gtgagaaccg caccgacagt gtggaaatta ccgccgtacg
    18061 tcagcgcctc aaaataaggc tgccagtcga ggctgacgtt cacggtattg ataatgagat
    18121 caaactgccc cgccagtgct ttcagtgcct gcggatcgcg gctattcacc actttatcgg
    18181 cacccatcgc cagcaattcc tgctctttcg ccggattaga actaaaggcc gtcacctcac
    18241 atcccattgc gtgcagaagt tttatagcga tatgccccag cccgccaata ccaattaccc
    18301 caacgcggct ggtagcagtg atatggtgca tcaacagtgg tttaaagacc gtgataccgc
    18361 cgcacaacag cggcccggca gattcaatgt cgatattttc cggcagtgga ataacccatt
    18421 gccagtctac acgcaacttc tcggcaaaac ctccgcgatt cataattgtt ggcaccgcac
    18481 cttgctcaca gttgatctga tttccgctaa tacaggcgtc gcagtgacca cagctacgcg
    18541 ctgtccagcc aatcccgaca cgctgaccga cctgcaaacc tttatcctgc gcggcactcc
    18601 cgagcgccac cacgcgacca atcacctcat gcccggcaac cagcggatat tgtgaaaatc
    18661 cccattcgtt atcgatcatc gacagatcgg aatggcagat cccgcagtaa tccacctgca
    18721 cttcaacatc ttgtggcttc agctcaccgg gatcgtactc ataaacttcc agttcgccgc
    18781 ccgcttcttt tgcggcatag ctttttatca ttgacatttt ttggtccttc tctggtctgg
    18841 tctgggaagt gtagagcatg gcagggcggg gtgcatggag tgtgacaaag gttacacatc
    18901 gctgtacgca atgctgaaaa tttcagcact tagcgagatg cgagcaagct ggcgcttgca
    18961 tggtggcgtg cgacaggtat aatccacaac gttttccgca tacctcttca gtgccgaagt
    19021 ggcgaaatcg gtagacgcag ttgattcaaa atcaaccgta gaaatacgtg ccggttcgag
    19081 tccggccttc ggcaccatta gctcttccaa gaccatccga gaaagtccaa ttattcctta
    19141 aaaatcaatg attgcagcga tttttacgtc ctgagttgtc cgaggttgtc cgttgaaatc
    19201 cggatgtcat tgggggcata attgggggca tcttaacttc gattagaaat gtgcccccaa
    19261 atgaagctca gcgccagaca ggtcgagacc gcaaaaccaa aagataaaac ctacaaaatg
    19321 gccgatggcg gcggtttgta ccttgaggtt tcggccaaag gatcaaaata ttggcgtatg
    19381 aaatacagac gcccctctga caaaaaagag gatcgcctcg cttttggtgt ttggcctact
    19441 gtgacgcttg ctcaggcaag agcaaagcgc gatgaagcca aaaagctatt agtgcagggc
    19501 attgatccaa aagccgaaca gaaagaggct caggccgaga actcgggggc atatactttc
    19561 gaaaccattg ctcgtgaatg gcatgccagc aacaagcgct ggagtgaaga ccatcgatct
    19621 cgcgttcttc gatatcttga gctttatatc ttccctcata ttggttcgtc cgatattcgt
    19681 ctactcaaaa ccagccacct gttagcccca attaaaaaag ttgacgctag tggaaaacac
    19741 gacgtcgctc agcgcctgca acagcgtgta acggccatta tgcgttatgc tgtacagaac
    19801 gattacattg actcgaatcc ggccagtgat atggctggtg cgctatctac aaccaaagtg
    19861 cgacattacc ctgcgttacc ttcctgccgg ttacctgagt ttcttgcacg tctcgctgca
    19921 tatcgtggtc gtgtaatgac acggatcgca gtcgagcttt ccttactgac ttttgtgcgt
    19981 tccagtgagt tacgtttcgc gcgttggaat gagttcgact tcgataaagc cctttggcgc
    20041 gtacctgcaa agcgggagga aattaagggg gtgcgttact cgtatcgtgg tatgaagatg
    20101 aaagaggagc atattgttcc gcttagtcgt caggcgatga atttgttagg ccagttgaag
    20161 cagattagtg gtgataagaa actgcttttc ccgggcgatc atgacgcaac taaggttatg
    20221 agtgaaaaca cagtaaacaa cgcattacgg gcgatgggat atgacactaa aaccgaagtt
    20281 tgtgggcatg gatttagaac gatggcgcgt ggtgcactgg gggagtcggg gctatggagc
    20341 gatgatgcga tagagcgtca gttaagccat acagaacgaa ataatgtacg cgcggcttat
    20401 atccatactt ctgagcattt ggacgaacgt cgattgatgg tacagtggtg ggctgattat
    20461 ttggacgaga ataaactaag aatgataaca ccatttgatt acgggaaaat atctaatata
    20521 aagtgagttt ataaaaatca acttttgaag cggggggatt ccccc
//
LOCUS       DAZGSP010000090        19893 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZGSP010000090 DAZGSP010000000
VERSION     DAZGSP010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.090"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..554)
                     /locus_tag="ACHK74_003662"
     CDS             complement(<1..554)
                     /locus_tag="ACHK74_003662"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222409.1"
                     /translation="MSKKFTKTLLSSSLVGLLFVSAEGFAQVSVYPKDSQYSVLVDDE
                     NKPNKFGSKFKVYYSKSSHRGVVRYVQNGQDNVLFHFGNDGNIFVLSKDGQIGYTVHE
                     PVLKDFARMAEGLKSNGSKNHKLDEAEVQRIYDKIYNMSNTVVNPALKNQVGELSGRV
                     DKIDLAAGAIATDVASKTTVGVNS"
     gene            complement(915..9290)
                     /locus_tag="ACHK74_003663"
     CDS             complement(915..9290)
                     /locus_tag="ACHK74_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFP0222410.1"
                     /translation="MAFSDEQRPEAQLGNQNRSSLNIKQPGENYWQDFFSNPQNAIDH
                     STSFSLGDVLPTMGKGVAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPGV
                     ATVQDIFAGGSRGADEVIDTLPDGKNAVTDTVGKGLKATGKAVSDGAEATDEWLTGKM
                     SPGAVRALNTPMTEGYDDSAVWVAKGVNLIGALVPDMVAGGVARKVGDVTLRKMLTAG
                     LEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLNA
                     DYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPQLLAVN
                     AMAAKLGDAQLLNLAMRGTAKTVKSGIVRNATAQGAINAAQGGYSRYQENTALRETAG
                     MDVSPWEGVADATIEGAALGAAMGAPFGAVAGYRGRRQAAEETAMRDAETVQQDDAAP
                     QPESVDPVAQHRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQA
                     KRQAVMNELKAKPRSELLEEYRRLSQKEGRTETEEQQFQAIREVIRPQQEVTPEAQSQ
                     PENAEDGNGSIYPTVRFRDPNEVRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMGS
                     DVFRNAAATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERIADADPMEQ
                     PAFMRDPRFRGFTGDDTEVQARLARGNAPTAEELVRSQMAEGDAGPTAQELTERPRLP
                     APGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGQAPAPENAAGRQ
                     GKILEGETVRRGLPSPDAQNVTAPVREGLPAPDIARNVRMPQPESLPRTVRDSLPELA
                     QQAEVRRQAGGNRDIPQPETIAPESETTVSTDREATVRGGEVRGKKIEDFGEEIKGAA
                     KHRYAQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLANEGADADTLAMIALYRSDIP
                     AKTKHNTAGWGESIKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQLLRTLPP
                     SQMDRASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDELLTKAKV
                     WFAEQEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSGDIFIAYGKNKMRVRGGFKSASD
                     ARKYIDSHRDELVRHVKEMREISREEQRNATNRDRTGPERRKGNVSPEQFSDAFGFRG
                     VQFGNYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAAAH
                     YEPGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSTDGKITSGGDYMTEAQRAGRV
                     FKDGRYVDAEYPVRQEVYDAFKGVMKAINSSDMPRRSALLDEVRSKPYWSTDVEMAAR
                     AFERYVQDKARTAGVENDYLVNIRKAPEHNTDNTWAYPTNAELDGGIREAFDHLFSTL
                     KTRETDKGVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVMSGIKDSD
                     LKVRVVKSQKEAEALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHEI
                     IHHAMEHVVTPAEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLAH
                     MAEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTP
                     REDGAGASSQYSRSGKPDPFKVPEGEGERYREWLGRAMRSKRSSDIQIELGRTPPVLR
                     HLGAPDLPLVISRDTVRKATNGVKHVVPMDVIERLPELMHDPEAVYQSATHQNSLVIL
                     FNLRDRNGDPVISAVHLKAIKNGLEINKIASVYGTERGKKLSNMERQGLTLYRREKLT
                     PDEDLPRGLQLPVVEDSLGSLNKILYPEDIRKGPYYSRTSSLTPEETIASRFVRQMQD
                     KFQVLKAVQENIRKTGGKVDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLADY
                     KIAQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQY
                     DRLAGIVDDMLARRRELIREAGLEESGVVDAWQNTYRYYVPLKGQDVDGVVSLPRTGK
                     GFTIGGRESRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDKDY
                     WQVFTDDRPDTMRVIAERKDQEAGETIREVVERPVAMAMMADRYFTTKKNGKTYYIKL
                     HDPRLMRAMKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNL
                     KAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGKGAQWQKVWKEFVE
                     DGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWRGQSIGAWNSFLNLVEDANGAVENALR
                     LSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLVRT
                     LGHLNGEGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDKVP
                     SHVKERNLVIMKSMFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAGNV
                     VGGVLGAFSPIGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPSGT
                     PKPDSQLGRRSTPEAYKAFASWLNAFSGGSQYRPGAVDITPESLKYWVDYISGGTGRF
                     ISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAELKSL
                     TGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQSAMVKSVE
                     RDMKIAVDRFNREYNKKVGVE"
     gene            complement(9375..10637)
                     /locus_tag="ACHK74_003664"
     CDS             complement(9375..10637)
                     /locus_tag="ACHK74_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222411.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHRKENDQRTYDLNVRNADREYALKEREHKAAQNYRNASLGLSQQRLQLEKY
                     NQRRLEYNDMLARDQSVMVALGKAIDAGDHDAASRLFGKLSDANPIKIISTEGYAAKA
                     GQAVNNLQKIFDDKPDRAIDSLNTPENLDVFSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQNVLAIPVDKAIALVRD
                     RSKFAEISKNYGYFMPKQQGLSLKELQKEASKVVAQAIKDGRDAQAANDEFLAAYDSL
                     PHQQKVQQRKTQQLLQKWQPWAAGDPVKQEFVKEAAANAPALLEPGREKEVEIYYQNH
                     LRDKKAEHEKALDYSASAAAEKIKGWMK"
     gene            complement(10648..10899)
                     /locus_tag="ACHK74_003665"
     CDS             complement(10648..10899)
                     /locus_tag="ACHK74_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFP0222412.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRREEIEAANKQMAAQQKAQ
                     NKQNIGTGIGTGAAIGASVGGPVGAVAGAVIGGIAGSLF"
     gene            complement(10910..11356)
                     /locus_tag="ACHK74_003666"
     CDS             complement(10910..11356)
                     /locus_tag="ACHK74_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222413.1"
                     /translation="MEYGKYETLARAGYSGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSGLRTAQMAQDNQMARYGVNRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQQLSVGGQMMTA"
     gene            complement(11359..12012)
                     /locus_tag="ACHK74_003667"
     CDS             complement(11359..12012)
                     /locus_tag="ACHK74_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222414.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARKELASNLTRSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGAGGAKTDGTGANAIQHQAQNWRL"
     gene            complement(12107..12460)
                     /locus_tag="ACHK74_003668"
     CDS             complement(12107..12460)
                     /locus_tag="ACHK74_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222415.1"
                     /translation="MQRVVRNSRNPEFMNEVKDACFKKQAFCFEAPDGFLVLRSVLSA
                     DGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHTARRGFIRLGKRLGFER
                     MPDDEDGFMVFRIAV"
     gene            complement(12596..13792)
                     /locus_tag="ACHK74_003669"
     CDS             complement(12596..13792)
                     /locus_tag="ACHK74_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP0222416.1"
                     /translation="MKKASKLLLALMPALSLSLFSAGAYASGYSVTPETLNSNSMTSA
                     EFLKGIGVYPKEGGAPLIKLVDGKVTPLNGNTPTSLNIDAVSGKYLKYTLIDYGKAGL
                     TDTELVAPRGDIVSYYDYANETAYFVNTKTNQILFSADGEGVVSTVGADGKTYSRHIP
                     EISEYIYEIKMKAVQYGATIDTGLSAADVTDIHARLSSLGTGKTQVGGGATAGGENAT
                     AVGYNATANHTNSVAVGANSQTTRPDEVNVGNRVIGGLRDGVQAADAATYGQLERYRI
                     AEENARIQGDAQTLKSANTYTDNRVSTLERSTNQQFRQLRDEVEKNRKRADAGIAGAM
                     AMTAIPVVEGKTYSFGMAASNYRDEQAIAAGMHFRTTENSAVRLNASWDTQNGSGVAA
                     GMSVGW"
     gene            complement(14144..14284)
                     /locus_tag="ACHK74_003670"
     CDS             complement(14144..14284)
                     /locus_tag="ACHK74_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0222417.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            complement(14486..15106)
                     /locus_tag="ACHK74_003671"
     CDS             complement(14486..15106)
                     /locus_tag="ACHK74_003671"
                     /inference="COORDINATES: protein motif:HMM:NF023837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3672 domain-containing protein"
                     /protein_id="HFP0222418.1"
                     /translation="MNGDVTASNAKISGHIDADSGTLNNVMIEENCIIKGTMSADRII
                     GDIVKGYSFEPHDGTLHITNHKYGEGYVIFKAEVSLADDVSFDRKLILLPFTFYSGTL
                     TIEINGRVVIKADPRVANTGTAGYFDRYDNPVYTRTNLGNTVSGVWDVAAGGGDTRIT
                     VTMRVEGGPGNMGDVEGRKRELVRWRYDAFTLMVAKSTDSGFTRIS"
     gene            complement(15134..16252)
                     /locus_tag="ACHK74_003672"
                     /pseudo
     CDS             complement(15134..16252)
                     /locus_tag="ACHK74_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
     gene            complement(16249..17874)
                     /locus_tag="ACHK74_003673"
     CDS             complement(16249..17874)
                     /locus_tag="ACHK74_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222419.1"
                     /translation="MPYIDITTMRGMMPRVVTSMLPDHSAVLAEDCHFRFGVITPERQ
                     ISGVEKTFTIKPKTIFHYRDDFWFAWPDVVDVIRSPVAQDSHGRIYYTDGRFPKVTDA
                     TIGTKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLSPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPGKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDVNGNTALATEKIISPEQWQSQFNPASIVAYSWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGSS
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            complement(18055..18180)
                     /locus_tag="ACHK74_003674"
     CDS             complement(18055..18180)
                     /locus_tag="ACHK74_003674"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222420.1"
                     /translation="MKQANLKYVKKRYTAGAMCFMLFVWLGAVVTLCAVAAVMMQ"
     gene            complement(18224..18697)
                     /locus_tag="ACHK74_003675"
     CDS             complement(18224..18697)
                     /locus_tag="ACHK74_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222421.1"
                     /translation="MKVILATRNRYLEYGLQQMLEGYSVILAREFFMPENRKHTPENN
                     ESWVIICDALLGRLMRCMFQGRRYLQLDAEEMTGRLDTYRKIRNGDWVQNTYARPLTM
                     SEMVVMFGYVYRESKPCHLAREMGINTKTVNTFLYLGLGKNGLRYRSVKHLVGLA"
     gene            complement(18786..19856)
                     /locus_tag="ACHK74_003676"
     CDS             complement(18786..19856)
                     /locus_tag="ACHK74_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFP0222422.1"
                     /translation="MLVSSGALALDIPVKDSDYLIRINDSNNELASVIDTKTNKEVLG
                     AINTTLGSVMTWNEQDAKKVEEFIKTLPPEQQHVGLQMAMQQILFPVDSPSLKLDKLN
                     SGTLTKADIDAVVSLKTDVSKAISIETAPAYVAAVRSGGNSDAAVAAAKDGPGVLQEY
                     NRIVTNIDQLNKDTTFAMDADGNITLDETAGTGERYGVKDVVAEVVEDTTVRVAEDGS
                     LTTARNTQTTKRVNEAVVDLDKSVRTNSQDIATNRKAIQSNSRQLQEHNARLNDHQRQ
                     IRENHEEMKRAAAQSAALAGLFQPYSVGKFNATAALGGYSDKQAVAVGVGYRFNEQTA
                     AKAGIAASDGDVSYNVGVNFEF"
BASE COUNT         4571 a         5806 c         4336 g         5180 t
ORIGIN      
        1 tcactgttca caccaaccgt agttttagat gcaacatcag ttgcaatagc tcctgcagcc
       61 agatcaatct tgtcaacgcg accagaaagc tcaccaactt gatttttcaa tgcaggattt
      121 acaacagtgt tagacatgtt ataaatttta tcgtaaatac gctgtacttc agcctcatcc
      181 agcttatggt ttttacttcc attactttta agaccttcag ccatacgggc aaagtccttc
      241 aacacaggct catgaacggt atatcctatt tgcccgtcct tgctcagcac aaaaatattg
      301 ccgtcattac cgaagtgaaa tagaacatta tcttgtccat tttgcacata tcgcacaact
      361 ccccggtgag atgacttact gtaataaact ttgaatttgc taccgaattt attcggttta
      421 ttctcgtcat ccactaaaac actatactgc gagtctttcg gatatactga tacctgtgcg
      481 aaaccctcag cactcacaaa cagaagccct accagagaag atgacagaag tgtctttgtg
      541 aattttttac tcattatgga tatcctgtta aattatctgt atacaaatca gccgcaacac
      601 ctcatactta taaagaaata tgattaatta cgactttatt caggatattc cgaaaataaa
      661 tttaaaaatt aatttaataa ctttaggatt tatcttacgt tattctgggc atacttactc
      721 aggcccacgc ctacaccgcc ggaacagaca tcagaaaaaa cttatcctca atcagacttg
      781 ctgtttcaca tctgcttaca ctgtaatttc tgatcaacat cacaacatta tccgaattta
      841 actgttcagt tcatctacaa tatttccctg cgtgacttta tatcagcccc gcacttccgt
      901 acggggcatg ttctttactc cactcccact tttttattgt actcgcggtt aaaccgatcc
      961 acggcaattt tcatatcccg ctctaccgat ttcaccatcg ccgactgttg cgccagactg
     1021 agagtactgt cggcgtaaat ggcatcacgc tgtttacgca aatccttcag tctcttccgg
     1081 gtatcctgca taagcccgtt catcgacaat tttccgttgt tctcgtcaat gaacgccgtt
     1141 ctttctgcac cggtcagact cttcagctct gcgtgatact gcgcaacctc tgtcatccgg
     1201 tcgtacatct tctgctggtc agcatacggc atcacctcac ctgaaatttt ccccaggaag
     1261 ggcacctgct gctccggtat atcaatacca ttcagcgatt tcaccgccgc atccgtggtt
     1321 ttggaaatga agcgccctgt ccctccggag atatagtcca cccagtattt cagcgattcc
     1381 ggtgtgatat ccaccgcgcc gggacggtac tggctgccac cagagaacgc attcagccag
     1441 gatgcaaacg ccttgtacgc ttctggcgtt gaacgtcttc ccagctggct gtcaggtttt
     1501 ggtgtaccag acggcatgtt ctcctggtaa atctgcgccc ccatgaagtt ttcattcatg
     1561 gcaaggttcg caaacggacg cagaatggtc ggcgctgcat ttttcagcaa tgccccggac
     1621 agagtttccg acgtctcact gccaatcggg ctgaatgcac caagcacacc accaacaaca
     1681 ttaccggcag cacgggacgc cgtcaggtca cccgccgcca caccttcagc ggtatgcccg
     1741 agcaggaaga aaacgttgta cccgtaaggc agaggaatac tccagtactc tccggccttg
     1801 cccccgaaca tcgatttcat gatgacgagg ttacgctctt tcacatgaga cggcaccttg
     1861 tcataccagt taaccccgtc atcatcctca cccgccacgc tgcggttaag cgagccaagc
     1921 agataacctg ctcccacagc ggcaagcgcg attttctgcg gaacattcag attcttccag
     1981 cgaaggcgct ccagtaaagg tccctcacca ttaagatgtc cgagcgttct caccagattt
     2041 gccgttccct gaatactggc gttggcgaac atgtacagcg agttcatcag cgccccctgc
     2101 tcaccacgac ggttaaagtt caccgtcatg tttttggcaa gagacgccgc ctgctggcgt
     2161 gacaaaccgg catcacgggc gtgtttataa gctgacagac gcagtgcgtt ttcaaccgcg
     2221 ccgttggcat cctcgaccag gttcaggaac gaattccatg caccgatact ctggcctctc
     2281 catcctccct tcgccagcga tacaaggcga tccatttcct tctgctggcc ttcaaggtca
     2341 cccatgttaa accagccggt tttacctccg tcctcaacaa actctttcca caccttctgc
     2401 cactgtgcac ctttgcccgt gagggttttg ccacgcagac tggcgtatac ggctgacatg
     2461 gcagaacggc tgtctttcac cacagccagt gcggataagt tatccagccc tttcagtttg
     2521 ccgtcgctcc ttccctgctc agccttcagg ttcatcaccg ccgtctgcac gtcacggatg
     2581 aagttactga ccaggaattc cgggttatac gacgtgttca ccgttgccag gaagcggtta
     2641 actttaccca gcgtacggat tacggcgtta ctggtttccg gtcccatatt cttcatcgca
     2701 cgcatcaggc gcggatcatg gagtttgatg tagtacgttt tgccgttctt tttggtggtg
     2761 aagtaccggt ctgccatcat tgccatcgcc accggacgct cgacaacttc gcgaatggtt
     2821 tcaccagctt cctggtcctt gcgctctgca atcacacgca tggtatccgg tctgtcatca
     2881 gtgaatacct gccagtaatc cttgtcggga ttatcctgca ccagtttcag gaaggcgtta
     2941 cccacttcgt ttttgcgatg gcggatcagc gattcgctca ggtcctgtat cgcctgagtg
     3001 gacggagact gtgcacggga tgcacgcccc atggcctgcc tgctttcacg tccgccgatg
     3061 gtgaagccct tacctgtacg gggcagtgac accacaccgt caacatcctg gcctttcagg
     3121 ggaacgtagt aacggtaggt gttctgccag gcatccacca caccgctctc ttccagtccg
     3181 gcctcacgga taagctcacg gcgacgggcc agcatatcgt caacaatccc tgccagacgg
     3241 tcatactgtg cctgttttcc actgttacgc acacgctgca tgatttccgc cgcttccgcg
     3301 ttggtcatcc ccgaaccgcc gtccggcatt ttcgggttga ttttcgcgat atgcgcgtta
     3361 cgttccggcg cgtgacgggc gtagaggtac tcatccagat cggcctgcgc aattttgtag
     3421 tccgccagta atttagccag cggctgaaca tagcgctcct tcatcacgtt caggtcgttt
     3481 tccgccttcc catggaagag ttcttccgcc atataagcgt tgttactgtc gtccactttg
     3541 ccgcccgttt tacggatatt ctcctgcaca gccttcagca cctggaattt atcctgcatc
     3601 tggcgaacaa aacgcgatgc gattgtctct tccggcgtca ggctgctggt gcgggagtaa
     3661 tacggcccct tgcgaatatc ttcaggatag agtattttat tcaaagaccc cagtgagtct
     3721 tccaccacgg gcaattggag cccccgggga aggtcctcat ctggggttaa tttttctctt
     3781 ctatacaggg ttaagccctg gcgctccatg ttgcttaact ttttccctct ttcggttcca
     3841 tatacagaag ctattttgtt tatctccagc ccatttttaa tggcctttaa gtgcactgca
     3901 cttattactg gatcaccatt tctgtctctg agattaaaca gaataacaag agagttttga
     3961 tgagttgcgg actgataaac ggcttctgga tcatgcatca gttccggtag tctctcgata
     4021 acatccattg gcaccacatg tttcacgcca ttggtggcct tacgcacagt gtcgcgggaa
     4081 ataaccagcg gcaagtccgg cgcaccaaga tgacgcaata caggaggtgt gcgtcctagc
     4141 tcaatctgaa tatcagatga acgtttggag cgcatggctc ttcccaacca ttcacgataa
     4201 cgctcaccct caccctccgg tactttaaac ggatcaggtt taccgctacg ggagtactga
     4261 gacgatgcgc cagcaccatc ctcacgcggc gtgtaacctt cccgcacacg ctggcctaac
     4321 gtacggatgg tctcgcgaac aagtctgata tcgttcagtt ccgtcggctt cagtaacccc
     4381 gtgcgacgca gtaccccttt gaccagggca acaacacgct cccatgccgc cacaaattta
     4441 ttaggctgct tctctgccat atgtgccaga aattcacccg cctgcacttc cggtgattcc
     4501 ttaccataag acgcatcaac cttacgccag gcttcacgga tggtggcgtt atcactgtca
     4561 cgggttttca gcacggtctt aataatcgtc tgatattccg ctggcgtgac aacatgctcc
     4621 atagcatggt gaattatctc gtgacgcaac ttctcgcgaa cggtccgccc gtcagggatg
     4681 ttatccgcca ccaggacaat ttcacgttta tccggacgat agaacgcgtg caccctgccg
     4741 taaccgtcga atgattcccc cgccagcgct tcagcctctt tctgtgactt caccacacgg
     4801 accttcaggt cactgtcctt aatgccactc atcacgccac gggcaaccgc ttcaacctgc
     4861 gggaccgggc tgcctttggc ttccgcactg cggttaacat ccgaaatgag attgccttca
     4921 ggtgtgcggg tcacgccctt acgggaataa aacgcaacgc ccttgtccgt ctcacgggtt
     4981 ttcagcgtac tgaacaggtg atcgaatgcc tcacgaatgc cgccatccag ttccgcattc
     5041 gtcggataag cccaggtgtt atctgtgttg tgctcaggtg ccttacggat attgaccaga
     5101 taatcattct ccacgccagc cgtacgcgct ttatcctgaa cataacgctc aaaggcacgc
     5161 gccgccattt caacatccgt tgaccagtac ggttttgagc gcacctcatc gagaagcgct
     5221 gaacgacgcg gcatgtcact gctgttgatc gctttcatca cgcctttaaa cgcgtcgtaa
     5281 acctcctgac gtaccggata ttccgcatca acatatctgc cgtctttaaa tacgcgcccg
     5341 gcacgttgtg cttccgtcat atagtcgcca cctgacgtga tcttcccgtc agtggaaaca
     5401 tcataacgac caaaataatt atccagtgaa tggaaccatt cgtgggccag cgcccccggt
     5461 ccgttacctt ttgtcaggtt gattgccacc tcacccggct catagtgtgc cgccgcctta
     5521 cccttaccac gggcaccaaa tgccaggcca agacgaccgt tcagggaaag cgcttttgtc
     5581 ggtacattca gtacttccgc aaggtcatgc agcgagtcat aagcccggtt caaatcagcc
     5641 tgacgacgcg gaccttccac gtagttacca aactgcacac cacggaaacc aaacgcatca
     5701 ctgaactgct ccggtgaaac attccccttg cggcgttctg gtccggtacg gtcgcggttg
     5761 gtggcgttgc gctgctcctc acgcgaaatc tcccgcatct ccttcacatg acgaacaagc
     5821 tcatcacgat gtgaatcaat gtacttacgc gcatcactgg ctgacttaaa gccacctctc
     5881 acccgcattt tgtttttacc gtaagcgata aaaatatctc cactgcgggt attccggtaa
     5941 acgtcaaagc ggattttgtc atccggtgac ggtgctgttt tttcatcacc tttcgcctgt
     6001 gatttttcct cctgctctgc aaaccagact ttcgcctttg tcagtaattc atccctgctt
     6061 tccgagaaaa agaggttact ccccttattg tccttattgc gcagtgaata aagtttctgt
     6121 ggcggatcgt aacgcttccc tcctgccgcc tgatacacac ccgataccac ccgataagca
     6181 gaagccctgt ccatctgtga gggtggcaga gtgcgtaaca gttgccaggt atccgcgtaa
     6241 cgggagggca ttctaccttc catccattct gcgaggcgtt tcgcgctgac cgttccgttc
     6301 agcatttccg atacactgtg tcgtactttt tttatgctct ccccccagcc tgccgtattg
     6361 tgtttcgtct tcgccggaat atcgctacga tacagcgcta tcattgccag ggtatcagca
     6421 tcagcacctt cgttcgccag ttttgcgtag tccggtttcg ggaacagttt gctcagcggc
     6481 tgcgtggcat aatccctgtc ttccagcgtt ttacccagtg tttcggcaag ctgtgcataa
     6541 cggtgtttgg ctgccccttt aatttcctcg ccaaagtctt caattttttt gcccctgact
     6601 tcacctccgc gcacggtagc ttccctgtca gtagagacag ttgtttcaga ttcaggtgcg
     6661 attgtctcag gctgcgggat gtcacgattt cctccggcct gtcggcgtac ttctgcctgc
     6721 tgtgcgagct caggcagtga gtcccgtaca gtgcggggaa gtgattcagg ctgaggcata
     6781 cgaacattac gcgcaatgtc aggagccggt aacccttcac gtaccggagc tgtcacgttc
     6841 tgcgcatccg gtgacggcag accacgacga actgtttcgc cctccagtat tttcccctgg
     6901 cgaccagcgg cgttttccgg tgccggagct tgtcctttct ggaaactctg gcccttaacc
     6961 tcaccggttg tggtaaaacg accaccacgt ccggcctgat tttcatccgg cgtacgcgcc
     7021 acctctcccg gtaacggata tccctgtccg ggatgaatat cgccgggagc gggcaggcgt
     7081 ggacgctcag ttaactcctg tgctgtcgga ccggcatcac cttcagccat ctgtgaacgt
     7141 acaagctcct ccgccgtcgg cgcattgcca cgggcaaggc gggcctgtac ctccgtatca
     7201 tcccccgtga aaccacggaa tcgcgggtca cgcatgaacg cgggctgctc catcgggtca
     7261 gcatcagcaa tacgctctcc gtcagccagg gtgttaatgg tttccgttgc cacatcttca
     7321 gagaacgcag gattatccat ttcaacggca tactgattct cgcctttctt cactacggac
     7381 ggtttcaggc cggtggcggc ggcattacgg aaaacatcac tccccatggc gcttttctca
     7441 tccgtgaaat aacggttgtc cgggcgtacc ttttcaatgc gttccgctgg tctggacgca
     7501 ccattcccgt taatttcaat gcggacttca ttcgggtccc ggaaccgcac cgtcgggtaa
     7561 atgctcccgt taccatcctc cgcattttca ggctgtgact gtgcttccgg cgtcacttcc
     7621 tgttgtgggc gaatgacttc tcgtattgcc tgaaactgtt gttcttcagt ctcggtgcgc
     7681 ccctctttct ggctgagtct gcggtattcc tcaagcagtt cagaacgcgg cttcgccttc
     7741 agctcattca tcacagcctg tcgcttcgcc tgttcgtcca gttcattcaa caactggctg
     7801 gcagcttccc ggcgatgggc tgcggatgcg tcaccctctg ttgccatatc cgcatcagca
     7861 tactgctcca gaagctgctc gcgattcatc ccctgcatgg attcacgatg ctgcgccacc
     7921 ggatcaacag attctggttg cggggctgcg tcgtcctgct gcacggtttc agcatcacgc
     7981 atggcggttt cttctgcggc ctgacgtctg ccacgatatc cggcaactgc accgaatgga
     8041 gcccccatcg cagcaccaag ggcagcacct tcgatcgttg cgtcagccac gccctcccat
     8101 ggcgatacat ccattccggc ggtctcacgc aatgccgtgt tttcctgata gcgtgaatag
     8161 ccgccctgcg ccgcattaat cgccccctgt gcagtggcat ttctgacaat gccgctttta
     8221 acggtcttcg ctgtgccccg catcgccaga ttaagcaact gagcatcgcc cagttttgcc
     8281 gccatggcat tcacagccag caactgagga tcggttgcca gttgcgcacg cacctcatca
     8341 gccacacgct cttttgccag gtccattttc tggcgatcag taagctgtgc gtgctgcggg
     8401 tcggcgtcaa tggacaaaaa cgtctgctga aatttcggtg actgcgccag ctcagagtaa
     8461 tccgcattaa gaactgcatc tgctgccgcc attgcactct gcccctgtgc actggctgtg
     8521 gaatgggtga tcaggcccgc ctggaataaa tccggcattt ttttatcgac agcttctgct
     8581 gccagtgccg tggctctttc cggctgcatc cctgccgcga tgtatttttt ctccagcccg
     8641 gcggtcagca tttttcgcag tgtgacatca cccacctttc tagccacacc gccagcaacc
     8701 atatcaggta caagcgcacc aatcaggttt acacccttcg ccacccagac cgcagaatca
     8761 tcatagcctt cggtcatcgg cgtattcagc gcacgaactg cacccggcga catcttaccg
     8821 gtcagccatt catcagtggc ttcagcacca tcactgacag ccttaccggt tgccttcaga
     8881 cctttaccga cagtatcagt aaccgcgttt ttgccatcag gcagggtatc gatgacctca
     8941 tcagcccccc tgctgccacc ggcaaaaata tcctgcacag ttgcgacccc cggcagcccc
     9001 atacggctga actcatttaa aatacgcgcc cctgttttta ccgggctttg aatcatcgca
     9061 tcaccgagtc cacgggccat ttcccctgtt ccccggacgg actgggcaac acctttaccc
     9121 attgttggca atacatcacc gaggctgaac gatgtgctgt gatcaatggc attctgcggg
     9181 ttgctgaaaa aatcctgcca gtaattttct cccggctgct taatgttcag gctgctgcgg
     9241 ttctggttac ccaattgcgc ttcagggcgc tgctcatctg aaaaagccat acagactcca
     9301 gtaaaaaaag cccgacacaa tggtcgggcg taagtgaacg atattaacgg ggagcgcgtt
     9361 actgccgcat atctttactt catccagcct ttgatcttct cggcagctgc ggaagcgcta
     9421 taatccagcg ccttttcatg ctctgccttt ttatcccgga ggtgattctg gtaataaatt
     9481 tccacctcct tttcacgacc aggttccagc aaagcaggcg cattggcggc agcctctttt
     9541 acaaactcct gtttaaccgg atcgcccgcc gcccacggct gccacttctg taaaagctgt
     9601 tgcgttttcc gttgctgaac tttctgttga tgcggcagtg aatcatacgc tgctaaaaac
     9661 tcatcattcg cagcctgagc atcacggcca tccttgatcg cctgagccac tactttgctg
     9721 gcttccttct gaagctcttt cagagaaagt ccctgctgct tcggcatgaa ataaccataa
     9781 tttttcgata tctctgcaaa tttgctgcga tccctgacaa gagcgatagc cttatcaacg
     9841 ggtatcgcca gcacgttttg atcatcaggg tgcgcactgc catattctgt tacaggttta
     9901 tgcgcagtgg agccatcgct gtatgtgaga tcaaggccaa taagtacgta cccttcctgc
     9961 tgcgccggta cgatactgcc aatcctggcc tcttttattg tcttttcccc ggttgaatca
    10021 ggcatgccaa tacgctgttg tagttccggg gcaaacacgc cggaaaacac atcgagattt
    10081 tccggggtat tcaacgaatc gatcgccctg tccggcttat catcaaagat tttctgcagg
    10141 ttattcacgg cctgacccgc cttcgcagca tagccttccg ttgatattat tttaatgggg
    10201 ttggcatccg ataatttgcc gaacagccga gaggccgcat catggtcacc tgcatcaatc
    10261 gctttcccca gcgccaccat caccgactga tcgcgtgcca gcatatcgtt atactcaagc
    10321 cgtcgctggt tatatttttc cagttgcagt ctctgctgag atagcccaag tgacgcattt
    10381 cgataattct gcgctgcttt gtgttccctc tctttcagcg catattcacg gtcagcattc
    10441 ctgacattaa gatcatatgt acgctgatca ttctctttcc ggtgctcaaa ctgagactgc
    10501 gcaaattcaa aatcgcgctc gttatttttc tgctgctgag caagctgtgc ttctcgcaaa
    10561 ccaagctcct tacgacgggt cattgcctgg tcaacagtgc tgaatccggc aagtaacccc
    10621 tgtgcaaatc cgctcattca ccactcctta aaacaaagaa ccagcaatgc cgccaattac
    10681 tgcaccagca acagcgccaa caggaccacc aacggatgcg ccaatagccg cccccgtacc
    10741 aatgcccgta ccgatattct gcttgttctg cgctttctgc tgcgccgcca tctgtttgtt
    10801 cgcagcctca atttcttcac gtcgtcggtc tgcgtcactt attccctgta atgcctcacg
    10861 ccgtgactga ttcgcaatat ccagtaagcc gtaccccata tcttccccct tacgctgtca
    10921 tcatttgacc gccaacactc agttgttgtc ttgcaggtgc agaagccccc gtcagaatat
    10981 tcatctggcg atcctgttcg gcttcacgga taccattttt cgcgccagca attgccaggg
    11041 cattacgtaa ccccagcgta ttactgtcgg gattatccgg gcggtttacc ccgtatctcg
    11101 ccatctggtt atcctgcgcc atctgcgctg tacggagacc ggaagtggca aggcctccca
    11161 cccgtgcaag ctgtgcattc atcagactgt tgttctcccc aaggtcagcc aaccttgcta
    11221 cacggggtaa atatctggtg cgccagtcgt cgtattgctg gcgggtcagc gctgcggatg
    11281 tctgccagtc accctgcgga cgggccgcgc ctgaataccc ggctcttgcg agcgtttcgt
    11341 atttaccgta ctccataatc acagtctcca gttctgagcc tgatgctgaa tggcgttagc
    11401 gccggtgcca tctgttttag cgccgccagc tccgctaccg ccagccttat gcatcgcgta
    11461 cgcacctgcc gcacccagac cagcgccaac aagagaggcc cgcccctgct gttttgtaaa
    11521 cgccgcctgt gcatccgatt tagcttttgc cagactgctg tctgccagag agttaaaact
    11581 ctgtaacgca tccgccttct gaccagaacc gagagcagca acatcctgta gcccggcaac
    11641 atacttatct gcctgcgaca cctgcccccg tgtggttgtg tcaatctgcc cggttacctg
    11701 atcactctgg ttcgcattca ttaccgcgtt aaagcgacca ctggacgggt caacaccgga
    11761 ccgagtgaga ttacttgcca gctccttccg cgcttcgcca aactgttttt gataccccag
    11821 atttgtggtg ccagcaatat tgtcgtactg ctgctcactg ttcaggtcat cgaccttttc
    11881 catgaagtta tcttcagccg ggcggaggat atttttgtaa tcctgccacc ctttccaggc
    11941 cacttcttcc tgtgctattt gcgctgctgt tggttttact ttggtatcac caccaccttt
    12001 acttccaccc ataatggccc cctggataat aaaaaaaccc tgccggagca gggtcagaat
    12061 gtgaattgca atgttggttt aaacacgatg atgaaaactg tggcagtcag accgctatcc
    12121 tgaacaccat gaagccatcc tcatcatccg gcattcgctc aaagcccaga cgttttccca
    12181 gccggataaa tccccgcctt gccgtatgaa attcagccca acgtccgcca gccagacggg
    12241 ttaatgtctt cacctccggc agataacgct caacactgtt actccccgta cacacgccca
    12301 gcaacaccag aacataaggg ataccatcag cactgagcac agaacgcagc accagaaagc
    12361 catcaggtgc ctcaaaacaa aacgcctgct ttttaaagca ggcgtcttta acttcattca
    12421 taaattcagg gttgcgggaa tttctcacca cacgctgcat ataccggaga attttttcac
    12481 tcacactctc accacaaaaa agaacaatgt gactgtgctg tgtcttcagc gacagaagga
    12541 tgacggagaa cgcttccgat aaccaacacg gctggcgttc tccgcaaaaa ataaattacc
    12601 agccgacaga catacccgca gcaacacctg aaccgttctg ggtatcccat gaagcattca
    12661 aacgtacagc agaattttca gtggtgcgga agtgcatacc tgctgcgata gcctgctcat
    12721 cacggtagtt acttgcagcc ataccaaagg agtacgtttt accctcaaca acaggaatgg
    12781 cagtcatcgc cattgctcct gcaatacctg catctgcacg tttacggttt ttctccacct
    12841 catcacgtaa ctgacggaat tgctgattag tactacgctc cagggtactg acccggttat
    12901 ctgtgtaggt attagcagac ttcagtgtct gtgcatcacc ctgaatacgg gcattctctt
    12961 ctgcaatacg atagcgctct aactgaccat aggttgcggc atccgcagcc tgaacaccat
    13021 cacgaagccc accaattaca cgattcccca cattaacttc atcggggcga gtggtctgag
    13081 agttagcccc tacagcaaca ctgttcgtat ggtttgcagt ggcgttgtaa ccgacagcag
    13141 tggcgttttc tccgcctgct gttgccccgc caccaacctg agttttaccg gtgcccagag
    13201 aagacagacg ggcgtgaata tctgttacat ctgcagcaga aagaccagtg tcaatagtgg
    13261 ctccatattg cacagccttc attttaattt cataaatata ttcactaatt tcaggaatat
    13321 gtcttgaata tgtttttcca tcagcgccta ctgtcgagac aactccttcg ccatctgcac
    13381 taaacagaat ttgattggtt tttgtgttta caaaataagc tgtctcattc gcgtaatcat
    13441 aatatgagac gatgtctccc cttggagcaa ctaactcggt atctgtcagg ccagccttac
    13501 cataatcaat taaggtatat ttcaaatatt taccggaaac agcgtctata tttaatgagg
    13561 ttggtgtatt cccgttaaga ggagtaacct taccatcaac aagcttaatt aaaggagccc
    13621 ctccctcttt tggatataca ccgatacctt tcaaaaattc cgcagaagtc attgaattac
    13681 tattcagagt ttcaggtgta acggaataac cactggcata cgctccagca gaaaataatg
    13741 acaggcttaa tgctggcatt aaagccaaca atagcttgct tgcctttttc atgatgtgta
    13801 cccttatgtt aaatcatgtt ataagtatgt tgacattcgg aacgcaaatg tcttcaggga
    13861 tactactcac aataacaagg aaaataaaat attaaaatca atgagttaag aaaaattaat
    13921 aattaaaatc acaaggatgc aatacgcatc aaaaacttac cctaaataac accagtatta
    13981 ctcagaaaaa acaaaacatt ttctgtagtt attatttacc attgataaat attacctctc
    14041 tctgtcagcc tgcattgtat aaaacataca ctacaaacgg gtgccatatc ggctgaacct
    14101 cagcaaccag ttgacgggga ctttcgtccc cgtcgcggtt ttcctactgc ttacactgta
    14161 agaacgccgc aaactccgct ccccacaaat tcagccggaa ctcacacagt gaaccgtgca
    14221 acatccagat ggtgaagata accgtcatgc aaattgtgac ggtgatgagc gatttttgcg
    14281 acataacgct tgtctcctga atggagaggc gctaacctac tacttgtgaa ggttgtacgg
    14341 tcagggcctc gggttaacgt taaagttgac tcggggcctt tccacatcag gccttcaggt
    14401 tcaccctcca gccatcagcc gaaaggcacc cacgcatact gtacggtttt tgtctccttc
    14461 cggcaatccg tattacaact gcctgtcacg atatccgggt aaaaccggaa tctgtcgatt
    14521 tcgccaccat gagagtaaag gcgtcatatc tccatcgcac cagttctctc tttctccctt
    14581 ccacatcccc catattgcca ggaccacctt caactctcat tgtcactgta atccgtgtat
    14641 ctccccctcc tgccgccaca tcccatactc ctgaaacggt gttcccgagg ttggtgcgcg
    14701 tgtaaacagg attgtcgtat ctgtcaaaat aaccagccgt tcccgtatta gcaactcttg
    14761 ggtctgcctt aatgacaact cttccgttaa tctctatcgt cagtgttcct gaatagaaag
    14821 tgaagggaag gagtatcaac ttgcgatcaa acgaaacatc atcagcaaga gatacctccg
    14881 ccttaaagat gacataaccc tctccatact tgtggttcgt aatatggagc gtgccatcat
    14941 gaggttcgaa ggaatatccc ttgacgatat ctccgatgat tctgtccgca ctcattgttc
    15001 ccttaatgat gcagttttct tcaatcatca cgttattcag cgtgccggaa tccgcatcaa
    15061 tatgaccact tatcttcgca ttactggccg tcacatctcc attcactgaa acgctgaaat
    15121 gttccccgct ttttatgctg ccatcggact caacctgaaa atttccgttc tgaataacgg
    15181 cactccggat aactggtgaa gtgatactta ccccggcctt tacttcatcc gccacaacct
    15241 tccgcgacac cagtgtttca atcaccgcgt cataaatcat cgctttcggg atcacaacct
    15301 tgccacctga taccgcaaac ggataggcgg tgttatccgg gttgttcggg tcaaagacaa
    15361 acaactgcga cgcagaaatt gcaacctgac ttacaggcct gccttcactg tcttttccgg
    15421 cgacaatccc gatccccgca gtgataccat caactcccgc tttttttgac cacattgcca
    15481 gaaacgcctc accgccttct ttatccagtt tagtgatgcg cttgtcgacc tcattaagcg
    15541 attcactggt tgacgaatcc agtgtgctaa tgcgggtttc aataccacca atcgttcttg
    15601 tcgtttcttc cctgagagtc cccacaactt cggttgtctt aattgcagca tcctttacag
    15661 cctgcccctg cgcgtttttt atttctttac gcagctcgga cacaaccggc gactttgcag
    15721 cctcatcgcg gatctggtca atgatggcct tcacgccgat ctgtgtttgt gcctgagtgc
    15781 ctttttcagc attccatgga cctttcactc ctgccgcgtt aacaaaacgt atccagtaaa
    15841 atcccgacca gccagggtca accggatcgc cgtaaacctg ccccggcgtc gtggcaacca
    15901 gcactgcatc agcaaggtca tcctccgtac cccgccagat ttcagtcagt gaatgtccgc
    15961 gataattagg catatcccat tcaagaagaa ccgagccaaa tcctcctgtc gccttaaaat
    16021 tcagcggttt tgtgggaaaa tcaacagtca ttaaagtact gtcaatctca atacccggat
    16081 tcagtgcata tgaggcacca cccgatgttc gacgccgggc gagtttaaga ccaaccagtt
    16141 cctcacgggt cacaaatgcg tggcgtccgt caccacgctg cccggtgcca atttccatgt
    16201 tctccacaac tgtggataaa tccttccccg cacgccacgg ttttctggtc ataccggcat
    16261 ctccgacatc gatgtactca gggttattcg ttccacctgc ccgaatccgg ataccatcac
    16321 ctgccagttt tgcccggttg ctgccggaag tctcaccaca cttcccttaa acgtacccgg
    16381 cgcaaaatga atcacaggaa catcatcagc cataatggtg atccccaccc gctcaggcgc
    16441 cggagatttc accctgatac aggaaaaaga ggttctttca ggtaatgaaa aaatttttga
    16501 atgccacctt atcgtggagg gcagagagct cccggcaagc actgacattt tgtctcctgt
    16561 caccacgcgc atcatatctt tcgcgagatc aacccatgcg cagtcaaacg gtgtactgag
    16621 ataacggata tccatgttca ccggactgaa tacaaacaca tcctgcttac catccggttt
    16681 cgtgtaacag gcaatgtact caccacgcca ggaataagcc acaatggacg ccgggttaaa
    16741 ctgactctgc cactgttcag gtgaaataat cttttccgtt gccagtgctg tattaccgtt
    16801 tacatcaaca gataccagac cgtttgtccc agcatagagt acgaatccct ccatcgccac
    16861 catacttctt cggctcaggc atgcctgcat ggaaggaatt ctggagccag aaattgtgga
    16921 aggcgatacc ccactgaaca gatagggctc cccctttgtc gccaccacca gtgacgttcc
    16981 cagcggacag atagccacaa tatcttctgc cgtcgtgtga cgattcactt ccggccatgc
    17041 atacggcaga tacgcttccg aaaacatcac ttcattaccg gcaaacccgg cggcaatacc
    17101 attagccatc aggcaaaggc ctgtcatatt ctctggcggc ggcaggtaat cccatgtcgc
    17161 cagagaaggt ccaaggtttt tccccggtat tttgtccgtg taactgagca cggatgcatc
    17221 cagttcagcc acaagtaaaa aatccgcttc tcctccacct gatgcagagc gataaatccg
    17281 gcggcgttta atactggcat tctgcaatgg caccggagac agcgtcagct gtaccgcagt
    17341 ccccggagta cggagtgtta cctccagaga cgccggacct ggcggacctt cttcaccata
    17401 atctgagaca aaggtttccg tataaaaccg ggtttcgtca tcattcgggt tatcgtcaga
    17461 aacatcaccg ccctgctgaa cagtacagac aggtgctgtc gtcggcgcgg ggatccccag
    17521 acgatacgat gatgtcgggt gattcccgtc cccttttgtg ccaatggtcg catccgtcac
    17581 tttaggaaaa cgcccgtcag tgtagtaaat acgcccgtgg gagtcctgag cgaccggact
    17641 gcggatcaca tccaccacat ccggccacgc aaaccagaaa tcatcacggt aatgaaaaat
    17701 tgtttttggc ttaattgtga atgttttctc aaccccggat atctgacgtt ctggtgtaat
    17761 aacaccaaac cggaaatggc agtcctccgc cagtacagcg gaatgatcgg gcagcatgga
    17821 tgtcacaacg cgcggcatca tcccacgcat cgtggtgata tcgatataag gcatagaaat
    17881 tcctttgact gcttcaggat taaaaatcag gggatttatg gtggaattcg ctaaaatcgc
    17941 gatcggaatc actgcaacag agtgttgcta tgatattctg gacaagaagt aacaactcac
    18001 taattgttat caaacgtctt tcttctggtc ggggagcatg ttgctcccct ttctttattg
    18061 catcattacg gcagccactg cacagagcgt caccactgca cccagccaca caaaaagcat
    18121 gaaacacatc gctcctgctg tgtagcgctt ctttacgtat ttcagatttg cctgtttcat
    18181 gacaatttac ctgattgctc ttcagtaccg gataacaata cggctatgcc agtcccacca
    18241 ggtgcttcac actcctgtac ctcaggccat ttttaccaag ccccagatac agaaaggtgt
    18301 tcaccgtctt cgtatttatc cccatttcac gcgcaagatg acacggcttt gactcacggt
    18361 acacataacc aaacatcacc accatctcgg acatcgtcag aggacgcgca tacgtgttct
    18421 gcacccagtc accgttacgg attttcctgt atgtatccag acgccccgtc atctcctccg
    18481 catccagctg aagataacgg cgtccctgaa acatacagcg catcagcctg ccaagcagcg
    18541 catcacagat aatcacccat gattcattat tctccggtgt gtgcttcctg ttctccggca
    18601 taaaaaattc ccgggccagt atgacactgt acccctcaag catctgttgc aggccgtact
    18661 ccagataacg gttgcgggtc gccagaatca ccttcatcgc atctcctcac tgtctctgaa
    18721 cacatgaaaa aaacggcagg ccaaaatccc gcacctgccg tcatgcttca tgtcactgtc
    18781 agcaattaaa actcgaagtt aacgcccacg ttgtaagaaa cgtcaccgtc gcttgctgca
    18841 atacccgctt tcgctgcggt ctgctcattg aaacggtagc ccacaccgac agcaacggcc
    18901 tgtttgtcac tgtaaccacc cagggctgcc gtggcgttga atttgcccac actgtacggc
    18961 tggaacagac cggcaagtgc tgcgctctgt gccgctgcgc gtttcatctc ttcgtggttt
    19021 tcacgaatct ggcgttgatg gtcattcaga cgtgcattgt gctcctgcag ctggcggctg
    19081 tttgactgaa tcgcttttct gttggttgca atgtcctggc tgttagttct tacagattta
    19141 tcaaggtcaa ccaccgcttc atttacgcgt ttggttgtct gcgtgttacg ggctgtggtc
    19201 agggaaccgt cttctgccac acgaacggtg gtatcctcaa caacttcagc cacaacatcc
    19261 ttcacgccat agcgctcacc tgtaccggcg gtctcatcca gtgtaatgtt gccgtcagcg
    19321 tccattgcaa aagtggtgtc tttattcagt tggtcgatat tggtaacaat gcgattgtat
    19381 tcctgcagaa caccaggacc gtctttagct gctgcgacag cggcatctga gttaccgcca
    19441 gaacggacag cggcaacata ggctggcgct gtttcgatag aaatagcctt tgaaacatcc
    19501 gtttttaagg atacaacagc atcaatatca gccttggtta atgttcctga attcaactta
    19561 tcaagcttta aactaggaga atcaactggg aacaaaattt gttgcattgc catttggaga
    19621 ccaacatgtt gttgctctgg cggcaatgtt ttaataaatt cttcgacttt tttagcatcc
    19681 tgttcattcc atgtcatgac actacctagt gtagtgttta tagcgcctaa aacttcctta
    19741 tttgtttttg tatcaataac agatgccaat tcattgttag aatcatttat tctaattaaa
    19801 taatcactgt ctttaactgg gatatctaaa gccagcgccc cagaactcac cagcatcagt
    19861 ccggcaacag cagaagaaag aattgtctta gag
//
LOCUS       DAZGSP010000091        19743 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZGSP010000091 DAZGSP010000000
VERSION     DAZGSP010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.091"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..132
                     /locus_tag="ACHK74_003677"
     CDS             <1..132
                     /locus_tag="ACHK74_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222423.1"
                     /translation="PVGNTVAVQQPAVDDEPDEEYERNFQRGLQLLEAKLKENDPLA"
     gene            204..1136
                     /locus_tag="ACHK74_003678"
     CDS             204..1136
                     /locus_tag="ACHK74_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0222424.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRTLFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            1139..1360
                     /locus_tag="ACHK74_003679"
     CDS             1139..1360
                     /locus_tag="ACHK74_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222425.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATEVYSGICRSVGAKQVS"
     gene            1373..1627
                     /locus_tag="ACHK74_003680"
     CDS             1373..1627
                     /locus_tag="ACHK74_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222426.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEE"
     gene            1629..1910
                     /locus_tag="ACHK74_003681"
     CDS             1629..1910
                     /locus_tag="ACHK74_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFP0222427.1"
                     /translation="MKRNLIAWAWSSGLIELGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            1907..2179
                     /locus_tag="ACHK74_003682"
     CDS             1907..2179
                     /locus_tag="ACHK74_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222428.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFAELVAMKASALMKDMDMSSGVINGNEFNQQG"
     gene            2184..2477
                     /locus_tag="ACHK74_003683"
     CDS             2184..2477
                     /locus_tag="ACHK74_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222429.1"
                     /translation="MSKVRVIFEFDHVMHEVKPAGNDSKEITEGVTATVKIERDTENR
                     PAGPCDVYAQILKYHSPTIIQFLTDELQGSMQAMGVSSSVERRSVQNGPDTLQ"
     gene            2489..3019
                     /locus_tag="ACHK74_003684"
     CDS             2489..3019
                     /locus_tag="ACHK74_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP0222430.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIHDWCKTNRNELTNGGETKTANLTTGTVSWR
                     LGNPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKQAVLAEPDAVKGVAGIKVNKGA
                     ESFHVEPFEQDAGLNK"
     gene            3117..3659
                     /locus_tag="ACHK74_003685"
     CDS             3117..3659
                     /locus_tag="ACHK74_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222431.1"
                     /translation="MAYFYFKLDRVQTNKYFTKYQQTVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGSLPAGWRKRDDVAFIGGGPCFIARPDESCPEGPAIAAMVEA
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNTDSWWTPSLSRDALCVVFKVGAYGREING
                     CIPEECQEIKHSEYVALTEE"
     gene            3663..4196
                     /locus_tag="ACHK74_003686"
     CDS             3663..4196
                     /locus_tag="ACHK74_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222432.1"
                     /translation="MIDAKVLEGVKNWLRIYGRLTCGVLAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQECAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITARVLRY
                     LIVDRTAAPAGISAEVA"
     gene            4196..4711
                     /locus_tag="ACHK74_003687"
     CDS             4196..4711
                     /locus_tag="ACHK74_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222433.1"
                     /translation="MFFKTSNPSALAAWKKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFCFKGLRFMDDKYPFHRDLWRKPTASNGWSCTPRTSRIPKALRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAILHGPVKWFCVDDVIYLQCEDDSAKRKMTEILSD
                     EFYAAEKRVRG"
     gene            4715..5266
                     /locus_tag="ACHK74_003688"
     CDS             4715..5266
                     /locus_tag="ACHK74_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFP0222434.1"
                     /translation="MMILQPMGRKGRAPAHVRAWTPEEDALLIALYPSTPVKDIAVRV
                     KRSFWGVHNRIVLLRGTYPELLKCKRPRFKHDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHKRARYIGVSMKKYGELLPFTRIPDDDVHLIRELRDAESPRRLTFREIGEKFELSE
                     STVNFIYHHRRTAEDVVLRELMP"
     gene            5263..5574
                     /locus_tag="ACHK74_003689"
     CDS             5263..5574
                     /locus_tag="ACHK74_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222435.1"
                     /translation="MITTLFVESDEPLVCAAGMSVCGGTLTGVYFGDLRGYPWHSLDD
                     AFPPDMEAVVLIVQYGDQQELRIGHMGYEGFFIDEETGACLEDEDGQVTHWCHISALP
                     E"
     gene            5589..5939
                     /locus_tag="ACHK74_003690"
     CDS             5589..5939
                     /locus_tag="ACHK74_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222436.1"
                     /translation="MRSYIDNEKLKTISDCLSLLAKIKETIEEIKFQLEYELCGDDTW
                     RNSARKALAVFQKQRRTVEYRLAVLRQEEKERNIRRHELVNDFLVRELKERVPESVFF
                     ECEAVARSKALETD"
     gene            5955..6287
                     /locus_tag="ACHK74_003691"
     CDS             5955..6287
                     /locus_tag="ACHK74_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222437.1"
                     /translation="MSIELRSSYEYRKILIAGGMKPEDAEKIVSFMDKECDKRDMPEI
                     IMDDMILHSAVALSPLWIVHSLAEIAKGTDKQAAVAALQTLNEMRISPRPTLIHMILS
                     SMEDKANE"
     gene            6280..6477
                     /locus_tag="ACHK74_003692"
     CDS             6280..6477
                     /locus_tag="ACHK74_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222438.1"
                     /translation="MNSLPAGWARPLMSRKHHFFKTGENISICGRWLYLAHNREPDTF
                     ESPDDCAECRRRLNKEKDNGQ"
     gene            6467..6763
                     /locus_tag="ACHK74_003693"
     CDS             6467..6763
                     /locus_tag="ACHK74_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001509933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222439.1"
                     /translation="MDSSSQLFWYLYITSFFGAFVITRWLCRKVISIFHKKYLIGQAA
                     TSLINALARYHKGECYSRIKTDDGFNIFVIPPEYRVQLDRENNTYHIRRSVDTA"
     gene            6760..7269
                     /locus_tag="ACHK74_003694"
     CDS             6760..7269
                     /locus_tag="ACHK74_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFP0222440.1"
                     /translation="MRGKLISAIHVAKRELALDDETYTSVLLAVTGKTSCRDMSPDEL
                     SRVLDVFKKRGFKVRQNPVNRALKPGTVTAKIRAIWKVMHRQGFISDGAETALNRWVK
                     SQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMGMPERTLMGYDAVC
                     RQYEKSLPR"
     gene            7339..7764
                     /locus_tag="ACHK74_003695"
     CDS             7339..7764
                     /locus_tag="ACHK74_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFP0222441.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDVLKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGGRGYYLPVGESLFAELRNNEIFSRWD
                     RGEKIESLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
     gene            7836..8336
                     /locus_tag="ACHK74_003696"
     CDS             7836..8336
                     /locus_tag="ACHK74_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0222442.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENSTYIDP
                     TGTPTICYGHTGPDVKPGMTLTDEECLELLEKDMKWAFAAIDRRVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            8371..8799
                     /locus_tag="ACHK74_003697"
     CDS             8371..8799
                     /locus_tag="ACHK74_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222443.1"
                     /translation="MCGTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQHSEEQQSV
                     VRRSLAAVPAASVPVPDDVADRVRRAVCEIRACEAGADPR"
     gene            8783..9001
                     /locus_tag="ACHK74_003698"
     CDS             8783..9001
                     /locus_tag="ACHK74_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222444.1"
                     /translation="MPIPAEWLADCLVPPAPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            9012..9239
                     /locus_tag="ACHK74_003699"
     CDS             9012..9239
                     /locus_tag="ACHK74_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP0222445.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKRRAMDAPGRRVCADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARHVGQHR"
     gene            9220..9528
                     /locus_tag="ACHK74_003700"
     CDS             9220..9528
                     /locus_tag="ACHK74_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFP0222446.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTQ
                     "
     gene            9525..9815
                     /locus_tag="ACHK74_003701"
     CDS             9525..9815
                     /locus_tag="ACHK74_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFP0222447.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRVLINGYFIAELTGRGQDVAEGRACVPGVKKPRARG"
     gene            9818..10399
                     /locus_tag="ACHK74_003702"
     CDS             9818..10399
                     /locus_tag="ACHK74_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFP0222448.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKA"
     gene            10399..12063
                     /locus_tag="ACHK74_003703"
     CDS             10399..12063
                     /locus_tag="ACHK74_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222449.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLLEALNSRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILIARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAVNARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            12063..13652
                     /locus_tag="ACHK74_003704"
     CDS             12063..13652
                     /locus_tag="ACHK74_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFP0222450.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAVLDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIS
                     DPWIREQTGIPQPTPGEAIFRVRQSGNEPAQTDKEMPPEKQEKTEQTALAARLPEAKS
                     SPRDELDDMGDAVPARRLQEAIDPVLEPVIDAIRTRGLAEALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGTGD"
     gene            13636..14967
                     /locus_tag="ACHK74_003705"
     CDS             13636..14967
                     /locus_tag="ACHK74_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFP0222451.1"
                     /translation="MAQGIDLGYAATLASKEAVAYFRAKGAHISWNWFETDADVHARS
                     FTAAKVARLDVLTTLQAEVQRAVDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGLVFRYDDPFWNTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGQDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSMKHQNDGIALTPEDFGRLPAMLAAPKAVLWDKRHNNLMYIVESKDG
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGDID"
     gene            complement(14969..15048)
                     /locus_tag="ACHK74_003706"
     tRNA            complement(14969..15048)
                     /locus_tag="ACHK74_003706"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            15089..15562
                     /locus_tag="ACHK74_003707"
     CDS             15089..15562
                     /locus_tag="ACHK74_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFP0222452.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVASSLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            15738..16871
                     /locus_tag="ACHK74_003708"
     CDS             15738..16871
                     /locus_tag="ACHK74_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFP0222453.1"
                     /translation="MKTENHPKDANRPSGLAVLSASLTASGDGWCQLLPAGRVKARDG
                     RPEKPAEGWLINESACNRMKAGLSALNQPLLIDYDHHSMNAQKNGFKAIAAGWVKPEN
                     IEWREGQGIFIKPEWTPQAQKHIDDREYAYLSAYMHYFADTGEPYLLRMASLTNDPGI
                     TGMNPVAALSADDLYVVSPSQEQHPMNEQLRQLLTALGLTVADGDEFTPELGTAALSA
                     LTGIKTRADAHDNLKTQVASLSAELETAKGTPAGGSIDLTKYVPVETYNALRTEYVAL
                     SAQHGSTTLEQVLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAALTGL
                     QTDTVTVPDKKTATAALSAEDIAAAHLLGKTEAEFLKMKEEMQ"
     gene            16871..17818
                     /locus_tag="ACHK74_003709"
     CDS             16871..17818
                     /locus_tag="ACHK74_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFP0222454.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAVQPDELVFAALRDGISAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNATVSNEMKGKLELVVADYL"
     gene            17862..18251
                     /locus_tag="ACHK74_003710"
     CDS             17862..18251
                     /locus_tag="ACHK74_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFP0222455.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVTPEQLARLEAEPCLTVRILQTPADDAGSVAGVVHAVAGTDLAEAEAEAEA
                     EAEAEAGQDAPRKKTGNKAKQAKKARA"
     gene            18248..18667
                     /locus_tag="ACHK74_003711"
     CDS             18248..18667
                     /locus_tag="ACHK74_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFP0222456.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            18664..19224
                     /locus_tag="ACHK74_003712"
     CDS             18664..19224
                     /locus_tag="ACHK74_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFP0222457.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDISSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            19225..19470
                     /locus_tag="ACHK74_003713"
     CDS             19225..19470
                     /locus_tag="ACHK74_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFP0222458.1"
                     /translation="MKTIFIKPEPGRLIRDPDTMRPLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            19467..>19743
                     /locus_tag="ACHK74_003714"
     CDS             19467..>19743
                     /locus_tag="ACHK74_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0222459.1"
                     /translation="MINFDGIGNDRRIPLIEVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGAGVLDIPVRITRASQATEFWGRGSMIALMVAEFIAINPDAE"
BASE COUNT         4732 a         4911 c         5742 g         4358 t
ORIGIN      
        1 ccggtaggta atacggtggc cgttcagcaa cctgccgttg atgatgagcc tgatgaagaa
       61 tacgagcgta atttccagcg gggattgcag ttgctggaag cgaaattaaa agaaaatgac
      121 ccgctggcct gaaataaaaa aataacccga gcggcgactc aggttatttg attaaacaag
      181 gtatcaaatg agaggttaat aatatgactg atattaacga tgtaatcaag accattgatg
      241 aacttattga tggcggcgta ctgacgcagt atgccatcgc cagagaggcg ggaatttccg
      301 acggcacatt atcggctttc cgtaagggaa aatataaagg cgataacgct gctgtggctg
      361 cttccctgcg ttcctggtat gagaactgga ataaacaaag cgcactgccg gaaccgccgc
      421 agtttgtgga aacgcagaca gtccaggagc tgcgcacact gtttcaggcg gttcgtctga
      481 tgggctgtat taacgttatt gtgggcgtac cgggtgtggg taaaacggcc actgcccgta
      541 attactgcca ggagcaacca aacacctgga tgatcaccct gtcacccgcg cactccagcg
      601 tcacggagtg tctgctggag ctggccgatg cgctggggat tgattacacc cgcgcgaaca
      661 aaggggcatt atcccgcgcc atccgccgtc gcctgatggg aacgcgtgga ctggtgattg
      721 tggatgaggc ggatcatctt ggtattgacg gtctggagca actccgggca attcaggacg
      781 ccacggggat cgggatggtg cttattggta acccgcgcgg attgtttaaa ggtggacgcc
      841 gcgcctttga tgatttatcg cgcctgttca gccgtcttgc ccgtacaaaa caacttcgca
      901 aggccaaaaa ggcggatgtg ctggccattg ccagggcgtg ggggatcagt ggtgaggccg
      961 agctggccgt catgcaggct atcgctgaaa agccgggagc gttacgcgtt ctgacacata
     1021 cgcttaacca ggcgtggctc accgccagcg gtgaaggcgc ggcgctgaca gaaaaacata
     1081 ttaatgcggc ctttaaagag gtttatacca accctgaatt actctcacag gtgtgattat
     1141 ggcggtattt aatattcctg atatttacgg acgcttttac ctggttaatt tcgataacgt
     1201 gaaggtgatt tcactggccg aaaataaaga atgtggcgat ttactttttg aatttaatga
     1261 ccgcacacga atggtgatat ctgccggact tgatcgcgaa ggtgcgacag aagtttacag
     1321 cggaatatgc cgttctgttg gtgcgaaaca agtcagctaa atgaggtgtt atatgaatat
     1381 gcaatcctgc ggtaacaaaa tgaatttatt cgactccctg aacagcgcgc gccgtctgac
     1441 cgaacttgcc ggtgcggtac tggaacgcag taagcgctat ccgcaacgtt ttgcactgaa
     1501 aaccacgccg ccggtaggca acgtgcaggg aaccggtgaa attgaaatca ccatacagac
     1561 caacggcctg cgccgccgtg tgaaggctac ccgcatcagc ggctgcacgg tttactggga
     1621 ggagtgaggt gaaacgaaat ctaattgcgt gggcgtggtc gagcggtctc attgaactgg
     1681 gttacgtcct gccggaaggt gcattgccga tagttgccgg aaagcctgcc acagtacggc
     1741 atgtgattga ggttatggcg cgtcatggac gtgatgaaca ggagcagtta ctggttccgg
     1801 ggataccgga agcggtgacg gaggaagaag cctttaatgc catgattcgg ttctgccgtg
     1861 aggtcagacg ccgggtcagt tatccaaaca gaacgaggac cagaggatga gtaaagtcgt
     1921 acgcattatt ttcgaataca aggagcacgt tatccataaa aacgctgatg gaacagtgcg
     1981 catgggggta agtctggaca tacgttcaac cgggataaag cagaaaggtg atggacccgc
     2041 catgattttt ggggtggtta tgctcgcgga aagcagagac tttgctgaac ttgtggcaat
     2101 gaaagccagt gcgctcatga aagatatgga catgagttcc ggggttatta acggtaatga
     2161 atttaatcaa caggggtaat tccatgagca aagtacgcgt tatttttgaa tttgaccacg
     2221 taatgcatga agtaaagcca gccggtaatg atagtaagga aatcacggag ggggttactg
     2281 ccacggtaaa aatcgaacgt gatacagaga acagaccggc aggaccctgt gatgtttatg
     2341 cacaaattct taaatatcac agccccacaa tcattcagtt tctgacagat gaactacagg
     2401 gttccatgca ggccatgggg gtaagcagct ccgttgaacg ccgttctgtt cagaacggac
     2461 cagatacatt gcaataagga aaaacaaaat ggcaaaacgc gttacaaaat taaaggccgc
     2521 agcagaggcg gcaccgcaga cccgtgaaga ggtcagccgc gatatccgca ccctgggcga
     2581 tattcagcga gaggcgctgc gcctggaaac ggcaatgaat gatgaagtgg cagaaatcac
     2641 cgcccgttat acgccgcaga ttgaaaacct taaaaaagaa atcaaagtgc tttttaaggg
     2701 gattcatgac tggtgtaaaa ccaaccgcaa tgagctgacg aacggcggcg aaaccaaaac
     2761 tgccaatctg accaccggaa cggtgtcatg gcggctggga aatccatcat gcagcgtcag
     2821 tcgtgatgtg gaaggtgtga ttgaaatgct gcgtcgtatg gggcttgagc gcttcatccg
     2881 cacgaaagag gaagtgaata agcaagctgt cctggcggag ccggatgcgg tgaaaggggt
     2941 tgctggtatt aaggtgaata aaggtgctga aagttttcat gtcgagcctt ttgaacagga
     3001 cgccggactg aataaataac accgcattaa atctttaaat atcacttcgt tttaattatg
     3061 gcgctcgcgt caggggactg cctgcgcctg taaactgaaa ataaggaaca ggaaatatgg
     3121 catattttta tttcaaatta gaccgtgtac agacaaataa atatttcacc aaatatcaac
     3181 agactgtttt accgctacgc aacagtattc ttcgggcatt actgaaaaat acaggcgctg
     3241 ccggattgcg cttaaagccg ttcgccatgg acgtaatcag tgagttttat ttttctggtt
     3301 ctctgcctgc gggctggcgt aagcgcgatg atgtggcttt tatcgggggc ggaccgtgct
     3361 ttattgccag acctgatgag tcatgccctg aaggtccggc gattgccgca atggttgaag
     3421 ccgctgaacg cgagttaaga aagcgtcctg atttccttgt ctggctctgt gaaaagctgg
     3481 gggtaatgag aatcccctcc atgtttaaca cggactcctg gtggaccccg tcactctccc
     3541 gtgatgccct gtgcgtggtg tttaaagtag gcgcttatgg cagggaaata aacgggtgta
     3601 ttcctgaaga atgccaggaa attaaacatt ctgaatatgt ggcgctgacg gaggaataat
     3661 tcatgattga tgcaaaagtg cttgaagggg ttaaaaactg gctgcgtatt tacggacgtc
     3721 tgacctgcgg tgttctggct gaaaaaatga atatgccgcc atcctcgatg gtttattttc
     3781 tgcgtgatgc ggttgatgcc ggggtgctga cggaatgtaa cggtttttat gatattccgc
     3841 gtccccgccc ggtgcagccg gttcgtcgca aatgcagcca ggaatgtgcg gctgatgatg
     3901 ttcagtggtg cagcttcaga aagtccctgc cgtggattga ggggcatgat attccgtcga
     3961 tggcgtggga atttgctcag ggcgtactga cctgcgaaac cgtttatgtg gtggctgaag
     4021 ttgatgagca ggccatgaaa gaaggcgtgc cccagtttgt gatggcgtat atcgacatac
     4081 gcctgggtgt catcatctgc ggtttaagcg gctggaatat caccgcgcgt gttctgcgtt
     4141 acctgattgt tgaccggacg gccgcgcctg ccgggatatc tgcggaggtg gcgtaatgtt
     4201 ctttaaaaca tcaaaccctt ccgcgctggc cgcgtggaaa aaatatcagc aggactgcca
     4261 gaaagttaag gatgaggcaa aacgccttga ggccgtgctg aatgttgcgt gccggtcggt
     4321 atttgtatcc ggtatcagtg gcttttgttt taaagggctg cgctttatgg atgacaaata
     4381 tccttttcat cgcgacttat ggcgaaaacc gactgcgtcg aatggctgga gctgcacacc
     4441 gcgcacatca cgtattccca aagccctgcg cgttgcctct gacgaactta acagtctgtg
     4501 gcgtgaatat tcgcccgtca cgtatgccag aaccgatgcc ctgttgttct ggctgggcat
     4561 tgacttttca gcaatattgc atggtcccgt gaagtggttc tgcgttgacg atgttattta
     4621 ccttcagtgc gaagatgatt ccgcaaaacg gaaaatgacc gaaattctgt ctgatgagtt
     4681 ttatgctgct gaaaagcgag tcagggggtg atgtatgatg attttacaac ccatggggcg
     4741 aaaaggccgt gcacccgctc atgtccgtgc gtggacacct gaagaagatg cactgctgat
     4801 tgcgctttat ccatccaccc cggttaagga tattgctgtc agggtaaaaa gaagtttctg
     4861 gggtgtacat aaccggattg ttttattacg cggtacttac ccggagttgc tcaaatgcaa
     4921 acgtccaaga tttaaacatg atgaagataa atttatccga aaaaatgcca gaacgatgac
     4981 cggtaagcaa ataggagaat atctggggcg agaccgggat tctgtccaca aacgggcgcg
     5041 atatattggc gtaagcatga aaaaatacgg agagttgctg cccttcaccc gtatacctga
     5101 tgatgatgtt catcttattc gtgaattacg ggatgctgaa tcaccacgac gtcttacctt
     5161 ccgggaaata ggcgagaaat ttgaattatc cgagagtacg gtgaatttta tttatcacca
     5221 tcgccggact gccgaagatg ttgtattacg ggagttaatg ccatgataac gacattattt
     5281 gttgaatcag acgagcctct ggtatgtgcc gccggaatgt cggtttgtgg cggaacgctg
     5341 accggtgttt atttcgggga tttgcgcggt tatccctggc attcactgga tgacgcattt
     5401 ccgcctgata tggaggctgt cgtgctgatt gttcagtatg gtgaccaaca ggaactgcgc
     5461 atcggccata tggggtatga aggctttttt attgatgaag aaaccggagc ctgccttgaa
     5521 gatgaggacg gacaggtgac gcactggtgc catatttcag ctttaccgga atgacgggag
     5581 gtgaatgcgt gagaagttat attgataatg aaaaactgaa gacaatcagc gactgtctta
     5641 gcttgctggc aaaaataaaa gaaaccattg aagagattaa atttcagctt gaatacgaac
     5701 tctgtggcga tgatacatgg cgtaactctg ccagaaaagc actggctgtc tttcagaaac
     5761 aacgccggac tgttgaatac cgccttgctg tattgcgcca ggaggaaaag gaaagaaata
     5821 taagacgtca tgaactcgtt aatgattttc ttgttcgtga attaaaggag cgtgtgccgg
     5881 agtcggtatt ttttgagtgt gaggctgttg cccgtagcaa agcactggaa actgattaaa
     5941 caggcaggtg aatgatgagt attgaattaa gatcgtctta tgaatatcgt aaaattctca
     6001 tcgccggagg catgaaaccg gaagatgcag aaaaaatcgt ttcttttatg gataaagaat
     6061 gtgacaaacg ggatatgcca gaaattatta tggatgacat gattctgcat tcagccgtag
     6121 cgttaagccc gttatggatt gtgcattctc ttgctgaaat tgccaaaggc accgataaac
     6181 aggcagctgt tgctgccctt cagacgctta atgaaatgcg tatatctcca cgcccgacat
     6241 taatacatat gattctgtcc agcatggagg ataaagcaaa tgaatagtct acccgctgga
     6301 tgggcgcggc cactaatgtc caggaagcat cattttttta aaacaggcga aaatatcagt
     6361 atatgtggac ggtggttata tctggctcat aatcgcgagc cggatacatt tgaaagccct
     6421 gatgactgtg ccgaatgccg cagaagactg aataaggaga aagataatgg acagtagttc
     6481 gcagttgttc tggtatttat atatcacatc ctttttcggt gcattcgtca ttacacgatg
     6541 gctgtgccgg aaagttatca gtatttttca taaaaaatac ctgattgggc aggcggcgac
     6601 ttctctgatt aatgcactgg ccagatatca taagggggaa tgctactcaa gaataaaaac
     6661 tgatgatggt tttaatattt ttgtgattcc tccggagtat cgggttcagc tcgatcggga
     6721 aaataatacc tatcatatcc gcaggagtgt tgatacagca tgagagggaa actgatatct
     6781 gccattcatg tggcaaaacg cgagcttgcc ctggatgatg aaacctacac atccgtacta
     6841 ctggcggtca ccggcaaaac cagctgccgg gatatgtcac cggatgagtt gtcccgcgtg
     6901 ctggatgttt tcaaaaaacg cggttttaaa gtgcgtcaga acccggttaa ccgggcctta
     6961 aaaccgggta cggtgaccgc taaaattcgc gccatctgga aggtgatgca ccggcagggc
     7021 tttatctctg atggtgcgga aaccgccctt aaccgctggg tgaaatcgca gacggccgcg
     7081 caaaacggcg gcgaaggtgt ggcaaactgg cagtggctgg agcaacaccc cgccctggcc
     7141 tcagatgtgc tggagcgtct caagcgatgg caccgccgca aaatgctggc cgcgatggga
     7201 atgcccgaac gcacgctgat ggggtatgac gccgtttgca ggcagtatga aaaatcactt
     7261 ccccgttaac cccaaatccc gccacaacgc gggattttta ttttaaactt accgggaacg
     7321 cgagaaccgg aggctgatat ggcagaaact cagatgagca tgtttggtgg tgacagtgag
     7381 caactgcacg cccttatcga ccgccttgat gacatccctg atgatgttct caaaaagaac
     7441 tggccgcgga ccctgtcaga actggttgaa gtcactggcg cagaacttca gcgtcagggg
     7501 attgagccgg tactggccgg taaactggca cgcaaggtgg ctgcggctca ggcagcctat
     7561 atgggcggac ggggttatta cctgccggtc ggggaatctc tctttgccga gctgcgaaac
     7621 aatgagatat tttcgcgctg ggaccggggc gagaaaattg aatcccttcg ccgccattac
     7681 cggatgtcag aaacccagat ttataccgtc atacgcgaac agcgccgtct gcatctggca
     7741 agaacgcaac cgccactttt ctgatattcc gcaaagcctg ccgcctttcc ttcaccgtta
     7801 cgctgactca gagaacatca tgagacagcg taacaatgcc aaaactcccc gcaccactgc
     7861 gtaaaaagct gattgccctt gttctggccg gtgccgggac gttcacgatt gccacgcatt
     7921 acaccggtta ctgggaaggg aaagaaaact ccacgtatat cgatcccacc ggtacaccca
     7981 ccatctgtta cggccatacc ggcccggatg tgaaaccggg tatgaccctg acggatgaag
     8041 aatgcctgga actgctggaa aaggacatga aatgggcctt tgcggccatt gatcggcgtg
     8101 ttcaggtgcc gcttacccgt ggtcagacgg tggcgctggc ttcgtggatc ttctgggccg
     8161 gtgaaacgaa cttccgcaac tccacgcttc tgcgcctgat caatgccggg cagatgcccg
     8221 cgtcctgtaa gcagtatatc cgctggattt attcaaaggg ggtgaaactc cccggccttg
     8281 aggcccgccg ttcggcggat gaatggttat gtcgctacga cttgccgaaa gtctgaaccg
     8341 cttctggcga ccgctcatga tagcgctgct gtgtgcggta ctgctgttac ggggtgtcct
     8401 gtggctgcgg tgaataatgc cttaccggcc ctgattgccg gggcgtgcat ggcggcgctg
     8461 gggattatcg ctgtactggt tgccgcgata tggggaatgc accagaagac acagcgcctt
     8521 gaagacaata atcaggtgct tgtgcgtgag cgggatgagg cgcgtcaggt gctggcaaat
     8581 cagcagcaca ccctgcaact catttcacaa atcagtgagg cggccacgaa tgaaaaacaa
     8641 cagaacattc agcacagcga ggagcagcag agcgttgtcc gccggtcgct ggcagcagtg
     8701 cctgcggcct ctgtccctgt tcctgatgat gtggctgacc gggtgcgccg ggccgtctgt
     8761 gaaatacgtg cctgtgaagc cggtgccgat ccccgctgaa tggctggctg actgcctggt
     8821 ccctcctgcg ccggagccgt tcacgtttgg ggcatcggtc acttacaacc tgcaactgct
     8881 ggcggtgatc aagaactgca acgtggacaa ggccagtatt cgtcgtctgg aggcgcggcg
     8941 acaacatgaa tttactgata tggccggaac gcctgctgtt ccggcaggaa agacgaagta
     9001 aggaaaaagg tatggatgat tcagatcgtg ctcaggctgt gatggagcgg ggatcagaac
     9061 gcgccctttg taaccggctg acacgtaagc gccgggcaat ggatgctccg ggcagacgtg
     9121 tctgcgcaga ctgtggcgga gagatccccg ccgcacgtct tgttgcggtg ccggatgcca
     9181 tccgttgtgt gaactgccag aacatcatgg aggcccgtca tgtgggtcag catcgttaaa
     9241 gattatgttg tgccgatcct ttcggcgacg gcaaccgctg gcgggatatt catggcgctg
     9301 atgcgcaaaa cgtttgtccc ccgcgaggcc tttgaaaaac tctccgaccg cgttgagaag
     9361 gtggaaaccc gcctgtcgtc actgccgaca gaagccgagg tcaaccgtct gaatgtggaa
     9421 atcgtgaccc tgcggggtga actgaaaacc acgaacgcca cgctccgctc tgtctcctat
     9481 cagaacgaac tgctgctgga gcaggctgta aggaaaaaaa cgcaatgagt gatttcatta
     9541 ctgaagatca gcgtctggtc attctgcggt cgctggcaga ttacaacggt gaactgggtg
     9601 aatccgtgct ccaggactgt ctggatgatt acggccaccg cgtgtcccgc gacaccgttc
     9661 acacccacat tgcctggctt gccgagcagg gactggtgcg caaacgcgtt ctgattaacg
     9721 gttatttcat cgcagagctg accggccggg ggcaggatgt ggcggaaggc cgggcctgcg
     9781 ttccgggtgt gaagaaacca cgcgcaaggg ggtaacgatg gacaagccga cgcgtggtcg
     9841 tgtacgcaag gtggatttgc tccccgacag catccgtaag ccgctgctgg aaatgctgcg
     9901 tgaaaaacgt ctgacgcagg tccagatccg cgaggaaatc aaccgtctga tccgtgaggc
     9961 gggcctgccg gaagagcagc agctttcacc ggcggccatc agccgggaag cctcccgtaa
    10021 tgagctgatt gcccgtaacc tgcgtgattt acgcgagcag acaaaagcca tgatggccga
    10081 actgggggac aagccaaccg gtgaaaccac agcgctgatt ctggaaatgt cccgcgccct
    10141 gatgtaccgc cgcctgcgtg ccgcgaccga atctctgaac agcgacagcg acgtggatat
    10201 gcgcctcatc aaagacatcc tcctgtcagc acagcgtgcg gaaagtgccg ccgagcgcag
    10261 cattaagcgt gaaaaagaaa tccgggccgc atttgctgaa gagatggcaa acgcggtcac
    10321 cgacgagctg cgcggcgtgg acgggatgag tgaacagctt gagtcccgta ttaaggggat
    10381 tctgttgggt aaagcctgat gaataacgcc gaagaactct cacccttact gacgaatacc
    10441 gtctccaccc gcaaaattga tctggccggt gaaaaagccc tgctgggcgt ggatgtgccg
    10501 gacagccttg atttgcccgg tgatatgccg gtgtttctgg attatcaggc ccgctggttt
    10561 gaggatgaaa gcgaggtctg catcgcggaa aaatcccgcc gtaccgggct tacctgggcg
    10621 gaagccgggc gtaacgtcat taccgctgca aaaccgaagc ggcgcggtgg ccgcaatgtg
    10681 ttttatgtgg ggtcaaagca ggagatggcg ctggaatata tttctgcctg cgccctgttc
    10741 tcccgtgcct ttaaccagct ggcagatgct gatgtgtatg agcagacctt ctgggatcgg
    10801 gataaaaaag aagaaattct gacctacatg atccgctttc cgaacagtgg attcaaaatt
    10861 caggcactgt cttcccgtcc gtcaaacctg cgcggccttc agggggatgt ggtgattgat
    10921 gaagccgcgt tccacgagtc actggatgaa cttctcaagg cggcaatggc gcttaccatg
    10981 tggggcgcgc gcgtgcgtat tatctccacg cataacggcg tcgataatct gtttaatcag
    11041 tacattcagg aagcccgcga ggggcgcaag gattacagcg tccaccgcat taccctggat
    11101 gatgccattg cggacgggct gtaccgtcgt atctgttatg tcaccggccg cgaatggtca
    11161 ccggaaagcg agcagaagtg gcgtgatgat ctctacaaaa acgccccgac ccgtgaggat
    11221 gccgacgagg aatacggctg tatcccgaaa aagtccggcg gtgcctatat tcctcacgca
    11281 cttattgaaa tggcgatgat ccgcgacatc ccgattctga cgtttgaagc cccggacgac
    11341 ttcatcagcc gtgccgcatg gctgcgtgaa tcggaggttt taacctggtg tgaagaacat
    11401 ttaaaaccgc ttttagaggc gttaaattcc cgttcccgct tcagcttcgg ggaagacttt
    11461 gcccgcaccg gtgacctctc ctgctttgtg ctgctggaaa tcactgaatc cctggcaaaa
    11521 cgcgaggtgt ttcgcgtgga gctgcgcaac ctgccgtatg cccagcagga gcaggtgatg
    11581 atgtacatcc tgacccgcgt tccggcgctg gtcggtgcgg cgttcgacgc caccggtaac
    11641 ggcggctatc tggccgaagc cgcgctgctg gcctttggcc cggacatcat cgactgcgtg
    11701 atgttatcgc cgaagtggta cggcgagtgg atgccaaaac tgaaggccga gtttgaggat
    11761 cagaatatcc tcatcgcccg ccatcagacc acgcttgatg atttgcgcca tgtgaaggtg
    11821 gtgaacggta tcccgcaaat cgacaagggg cgcacgaagg accagaacgc aacggctgtg
    11881 aacgcccgcc gccatggcga ttttgccgtg gcgctctgta tggcaaaccg ggcgtcatac
    11941 atggagggct tcatcctgga tgaatcagcc tgtcaggcgc tgccggaacg gtcgcgggca
    12001 atggagggcg gttatcgtga tgacgatgag gcatatcatg aatttgatcg ggggtgctgg
    12061 tagtgggacg cataattgat cttgacggaa aacctttctc ctttgatccg gagatgcaga
    12121 gtgccgtgct ggatattccg cagattgcca gccgttatat tgaacatccg gcctcgggta
    12181 tcaccccgaa ccgggcggcg cagtgccttc gcggggctga acgtggcgat ctgattgccc
    12241 agtccgatct ggccgctgac attgaagaaa aggataccca cctttttgca gagctgggca
    12301 agcgacgtct tgccattcag ggcgtgccct ggagtattga gccgccaccg aacgccagcg
    12361 cgaatgagaa aaaggacgcg gaaatgctcg acgaatatct tcattccgcc gactggtttg
    12421 atgccatgct gtttgatgcc accgatgcca tcctgaaggg ctattcctgt atggagattg
    12481 agcacgggat gctcggtaaa atgcacatca tccgcgccat ccgctggcgt gacagcgggc
    12541 atttctgcct taacccggat gatttgagcg aactgcggct gcgtgacggc agccatgccg
    12601 gggtggcgtt tcagcccttt ggctggatag tgcatcagtc gcgttcacgc actggatacg
    12661 gtggtgcgac ggggcttgtc agaacgctta tctggccgtt cattttcaaa aactattccg
    12721 tgcgcgatct ggctgaattt ctggaggtgt acggcctgcc gatgaaggtc ggtaaatacc
    12781 cgtccggggc aacaccggag cagaaaagcg ccctgatgcg ggcggtgatg gatatcgggc
    12841 gacgtacagg cgggatcatc ccggccggga tgtcgctgga gtttcaggcg gcagcgaacg
    12901 gtcaggccga tccgtttgaa accatgattt cgtgggggga gcgttccatc tccaaagcta
    12961 tcctcggcgg cacgctgacc acggaagccg gagacaaggg cgcgcgctcg ctgggtgaag
    13021 tgcataacga ggtgcgccgg gaaatcaggg attctgattt acgtcagctg gccgccacgc
    13081 tgaaccgcga tctggtgtat ccgctgtatg ccctgaacac cgcccacgcc atagatatcc
    13141 gtcgcctgcc gcgtatctgt ttccagacaa aagagccggg ggatatcacc aaaatcacca
    13201 gtgcggtgat gcagctcagt acggggatgg atatttctga tccctggata cgtgagcaga
    13261 cgggtattcc gcagcccacg cccggcgaag ccatcttccg tgtccgtcag agtggcaatg
    13321 aacctgctca gacagacaaa gagatgccac cggaaaaaca ggagaagaca gagcagacag
    13381 cgctggctgc cagactgccg gaagcgaaga gcagcccccg agatgaactg gacgacatgg
    13441 gggatgcggt gcctgcccgt cggttacagg aggccatcga cccggtactg gagccggtca
    13501 ttgatgccat cagaacgcgg gggctggcgg aggcactggc agacctgcct gccctttacc
    13561 gtgaaatgga tgattcccgc ctgatgacgc tgctcagtga tgccatgttt gctgcggaaa
    13621 tgaaggggat gctggatggc acaggggatt gatttaggtt atgccgccac acttgcctca
    13681 aaagaggcgg tggcatactt ccgcgccaaa ggggcgcata tcagctggaa ctggttcgaa
    13741 acagacgcgg atgttcatgc ccgttcattc acggcggcaa aagtggcacg cctggacgta
    13801 ctgaccacac tacaggcgga agtgcagcgt gctgttgatg aggggatttc acagaaagca
    13861 tttattcgca cgctgacacc ccgcctgcaa aagctgggat ggtggggaaa acagattgtg
    13921 gtggacagtg ccggtaacgc ggaagaagtg cagctgggca gtccccgccg tctggcgctg
    13981 atttacaacg tgaacacccg tgtggcttac aatgccgggc gttacacgca gatgatgaac
    14041 aacacggaca cgcatccgtt ctggcagtat gtggcggtga tggacagccg cacccgcccg
    14101 gcacattccg cccttaacgg gctggtattc cgctatgacg atccgttctg gaatacacac
    14161 tacccgccca atggctggaa ctgtcgctgc cgtgtccggc cattgtctca ggcccgtctg
    14221 gatgcgatgg ggctgtctgt ttcatccggt caggatcatc tctccacccg caatgttgag
    14281 gctggcgtgg ataagcagac cggagaagtc agagaaatgc cggtgaccac atattcagat
    14341 ggcaccagaa ccatgacacc ggatgtgggc tggtcatata accccggttc ggcggcgttc
    14401 ggcacagacc aggcgctgat ccgcaagctg atcgaagtga aaagcccggc gttacgggaa
    14461 atggtggttc aggagatgaa caacagcccg gagcggcagc tggcatttcg catctgggca
    14521 aaaaacatca tgaaaacccg gcgaggcggt aacgatatcc gcacgctggg ctttatgacc
    14581 gaaagcattg cgcaggcggt ggaaagccgg acgggaacgc caccggcccg cctgctggcg
    14641 atgagcggta aaaatgtgct ccatgcggac agcatgaaac atcagaatga cggtatcgcc
    14701 ctgacgccgg aggatttcgg gcgattaccg gcaatgctgg ccgcgccaaa agcggtactg
    14761 tgggataaac gccacaacaa tctgatgtat atcgtggaaa gtaaggatgg cagtgtgcag
    14821 attgccgtta atgcgccgta cagcctgaag cgtcagccgg ataaactgga tgtgattgtg
    14881 aatgcttacc gggttatcaa tatggataag ctgaaatcgg atatccgggg cggaatgctt
    14941 gaggtactgg aaggagatat tgattaacgg tcagtggcgg gggtcgaacc cgcatacatg
    15001 atccacccgg aggcaggcac cgactttacc agttaagcgt acactgaccg cctgtttatt
    15061 ttatattcat cctttatgcg gaggcaatat gtcatccatt gatgccgctg tcgttgttga
    15121 cgttacgcgt ctccagcggg tctttgcccg gcttcagttt gtgggtggcg gaaaagacct
    15181 ggcccgcagt gtggcgtcca gccttctgtc atcctcagaa atggcgtttg aacaggaaaa
    15241 agagccggac ggcgaacgct ggcatgactg gtcagatcct taccgcaagt ggcgtacccg
    15301 taagggatac atgcccggca aaatcctgac gctgaacggc gatctggccc gacgcctgac
    15361 cacggattat ggcgacacct gggcgctgat tggatcaaat gagccttatg cggccattca
    15421 tcagtggggt ggcctgcccg gtatgccacc gggaccggcg gccattggtg cacgcccgta
    15481 tatgggcttt gatcaggtgg cagagcagga gatcatggac gaaatcagaa aacgctttaa
    15541 aaaggccaca gaaacgcctt aattgtttaa ggtatgcaaa tgcattacct tacccccttc
    15601 aggcgcgtgt cgtgattttc taacctgtat ttaacgggct ttaaaatctg cgtgaagcgc
    15661 ctttgcattt ttccctgaaa atacttcctg aaatcccgca aaacccgccc tgaaaattcc
    15721 cggcttatgc tgccggtatg aagacagaaa atcacccaaa agacgcaaat cgcccctctg
    15781 gtttagccgt gctttcagct tcgctgactg catccggcga cggatggtgt cagttactgc
    15841 cagcaggcag ggtaaaggcc cgtgacggtc gcccggaaaa accggctgaa ggctggctga
    15901 ttaacgaatc cgcctgcaac cggatgaaag ccgggctttc cgccctgaat cagccgttac
    15961 tgattgatta tgaccatcac tccatgaatg cgcagaaaaa cggatttaag gccattgccg
    16021 caggttgggt taagccggaa aacatcgaat ggcgtgaggg tcaggggatt ttcattaaac
    16081 ctgaatggac gccgcaggcc caaaaacata ttgatgaccg tgaatatgcc tatctgtctg
    16141 cgtatatgca ttatttcgct gacaccgggg aaccctacct gttgcgcatg gcttccctga
    16201 cgaatgatcc aggtattacg ggtatgaacc ctgtcgcagc actcagtgct gacgatctct
    16261 atgtagtttc cccttctcag gagcaacacc ccatgaatga gcaattacgc cagttgctga
    16321 cggcgctcgg tctgaccgtg gctgacggtg acgaatttac gccggagctg ggcacggcgg
    16381 cgctgtctgc actcaccggg atcaaaaccc gtgcggatgc acacgacaac ctgaaaacac
    16441 aggtcgccag tctgtcggca gagctggaaa ccgcaaaagg cacaccggct ggaggcagta
    16501 ttgatctgac gaaatacgtg cccgttgaaa cgtataacgc cctgcgtacc gaatacgttg
    16561 cgctgtcggc acagcacggc agcaccacac tggagcaggt gctggacaag gctgaatccg
    16621 aagggcgcat cttcaaaagc gaacgcggct atctggaaca actgggtggg cagattggtg
    16681 ttgcggcact ttctgcacag cttgacgccc gacagccggt tgcggccctg accggccttc
    16741 agaccgacac cgtgaccgtg ccggataaaa agactgccac cgctgcgctg tcggctgaag
    16801 atatcgccgc tgcccatctg ctgggtaaaa ccgaagccga gttcctgaaa atgaaagagg
    16861 aaatgcaata atgcctaccc cgattacacc ggcgatgatc accgccctga tgacgggcta
    16921 ccgttctgat tttcaggccg ggatgtccat ggccccgtcg cagtacaaaa aaattgcgat
    16981 gaccgtgccc tccacctcga aatccaacac ctacggctgg ctggggcagt tcccgcagtt
    17041 ccgtgagtgg atcggctccc gcgtcatcga gaagatgaaa gcctatggct atgccatcgt
    17101 gaacaaaact tttgaaggca ctgtcgccat tagccgcgat gacttcgaag acgacaacct
    17161 cggtatttat tccccgttgt ttcaggagat ggggcgcgct gcggcggtac aaccggatga
    17221 gctggtcttt gctgccctgc gtgacgggat cagtgcggcc tgctatgacg gtcagaactt
    17281 ctttgatacc gaacatccgg tttaccccaa agtggatggc tccggggatg cgcagatggt
    17341 cagcaatatg tttgtggcaa aaaccggctc tgtcggcgca caggcggatt acagcggtcc
    17401 ggcctggtat ctgcttgact gttcacgcgc ggtaaaaccg ctgatttatc aggatcgccg
    17461 taaggctgaa ctggttgccc agaccaaagt cgatgaaggt cgcgcattca ccgataacga
    17521 atttgtgttt ggtgcatccg cccgtcgcaa tgtgggctac ggcttctggc agatggccta
    17581 catgatgcag tcaccgctga cgctggatgc actgtggcac ggctggtcag ccatgcgcga
    17641 atttaccgct gacggtggcc gcaaactcgg cattaaaccc acccatattg ttgtccccac
    17701 ctcgctggaa aaacaggcgg tgcagttgct ggagcgtgaa ctgttcgcag acggaaacgc
    17761 aaccgtctct aacgagatga agggcaagct ggagctggtt gtcgcggatt atctgtaaac
    17821 ggtgagccgg gcttacggcc cggcccctgc ggagggcaaa tatgaatgaa catcacactg
    17881 tggcagcaac agatgattca ggtcttcagg tcagtggtga caattctgtc acggtgctgg
    17941 cagaagtgcg ttgcagtcgt tcggcgtttc gtcgtgcggg gttcctgttc acgcgtggac
    18001 gtcagcaggt tgaggtcacc ccggagcagc ttgcccgact ggaggcggag ccatgcctca
    18061 ctgtgcgaat actacagacg cctgctgatg atgcggggag cgtggcgggt gtggttcatg
    18121 cagtggccgg tacagattta gccgaagccg aagccgaagc cgaagccgaa gccgaagccg
    18181 aagccggaca ggatgctccc cggaaaaaga ccggtaacaa agcgaaacag gcgaaaaagg
    18241 cccgcgcatg aattacgcca ctgaaaccga tatgcgggcg cgttaccgcg aggatttgct
    18301 caggccgtta ctggctgtgc cccgttcgga tgagccggac acgcgcaagc tgaaccgggc
    18361 gctgacagat gcgtcagccc ttatcgacag ctatctgtcc gcccgttaca cgctgccgct
    18421 ggaggttatc ccggctgttc ttgttcagca ctgttgtgcg attgcctttt attacctgtg
    18481 cgatcagcga gcctccgatc aggcgcgtga ccgttaccgt gaggcgctgg cctggctgaa
    18541 agatgtcatg aacggcaatg tgccggtcgg tgtggatacg aacggtgcgg cccctgaatc
    18601 cggggattta ccacaggttc agtctgatgc agcggtattc gggcgcaacc agaagggctt
    18661 catatgatta cggaaaccga acaggcatac atcgcccgta tccgtgagta tttcgggaat
    18721 gaactggtgt ctgttgacac gcatcccggc gactggagcg acagcgtact gcgcaccatg
    18781 ctgattaacg ccccggcgat ctacgttgcc tggctgggtg ccggtgaagg ccgtacccgt
    18841 ggtcgcctgg tcagtcactg ggtgttctac gtcatcggcg acatgctcaa cgggcgtgag
    18901 gccagccgtc ccggactgta tcagattgtg gcccggctga tcgccgtact taacggcttc
    18961 agaacagaaa aaacctcacc gctttacttt gaaaaggcgg tcaacggtta caccgaaacc
    19021 caggcggaca gtggtgcggt gatgtatgcg ctttatttct cctgcgagga aatgatcgca
    19081 ccgctgaccg atatcagctc gctggacgac ttcctgcgtc attacgaaac ctttgtggag
    19141 ccgcccggta cgccgccgtt tgaggcacat attgatttgc cggggaacac gacaacggcg
    19201 gtcgaaccgc cggaggaact gtaaatgaaa accatcttta tcaaacccga gccgggacgc
    19261 ctgattcgtg atcccgacac aatgcgcccg ctggcacagg agggggagga aaagcccttt
    19321 acgccgttct ggtgtcggcg tcttgatgac ggcgatgtta ttcaggctga aaaagccgct
    19381 gaagaggctc ctgctgtatc tgcggatgca acgactgcga cagaaaaacc ggtcgcccaa
    19441 ccggcatccg ataaggagaa accgcaatga ttaattttga cgggatcggt aatgaccgcc
    19501 gtataccgct gattgaggtt gagtttaata actccatggc cgtcaccggc actccggcac
    19561 agcgtcagtg tgtgctgctg tttggtcagg cgagaatgaa agagaatgcc gtggacggtg
    19621 ccggtgttct ggatattccg gtcaggatca cccgtgcgtc tcaggcaaca gaattctggg
    19681 ggcgcggctc catgattgcc ctgatggtgg cggaatttat cgccatcaat cctgatgctg
    19741 aac
//
LOCUS       DAZGSP010000092        19616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZGSP010000092 DAZGSP010000000
VERSION     DAZGSP010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.092"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            13..438
                     /gene="nudI"
                     /locus_tag="ACHK74_003715"
     CDS             13..438
                     /gene="nudI"
                     /locus_tag="ACHK74_003715"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFP0222460.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(477..1079)
                     /gene="ais"
                     /locus_tag="ACHK74_003716"
     CDS             complement(477..1079)
                     /gene="ais"
                     /locus_tag="ACHK74_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFP0222461.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            1387..2526
                     /gene="arnB"
                     /locus_tag="ACHK74_003717"
     CDS             1387..2526
                     /gene="arnB"
                     /locus_tag="ACHK74_003717"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFP0222462.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPVDIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            2530..3498
                     /gene="arnC"
                     /locus_tag="ACHK74_003718"
     CDS             2530..3498
                     /gene="arnC"
                     /locus_tag="ACHK74_003718"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFP0222463.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            3498..5480
                     /gene="arnA"
                     /locus_tag="ACHK74_003719"
     CDS             3498..5480
                     /gene="arnA"
                     /locus_tag="ACHK74_003719"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFP0222464.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYATDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            5477..6367
                     /gene="arnD"
                     /locus_tag="ACHK74_003720"
     CDS             5477..6367
                     /gene="arnD"
                     /locus_tag="ACHK74_003720"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFP0222465.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            6367..8019
                     /gene="arnT"
                     /locus_tag="ACHK74_003721"
     CDS             6367..8019
                     /gene="arnT"
                     /locus_tag="ACHK74_003721"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFP0222466.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            8016..8351
                     /gene="arnE"
                     /locus_tag="ACHK74_003722"
     CDS             8016..8351
                     /gene="arnE"
                     /locus_tag="ACHK74_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFP0222467.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            8351..8737
                     /gene="arnF"
                     /locus_tag="ACHK74_003723"
     CDS             8351..8737
                     /gene="arnF"
                     /locus_tag="ACHK74_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFP0222468.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(8731..8997)
                     /gene="pmrD"
                     /locus_tag="ACHK74_003724"
     CDS             complement(8731..8997)
                     /gene="pmrD"
                     /locus_tag="ACHK74_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFP0222469.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(9107..10462)
                     /gene="menE"
                     /locus_tag="ACHK74_003725"
     CDS             complement(9107..10462)
                     /gene="menE"
                     /locus_tag="ACHK74_003725"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFP0222470.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(10459..11421)
                     /gene="menC"
                     /locus_tag="ACHK74_003726"
     CDS             complement(10459..11421)
                     /gene="menC"
                     /locus_tag="ACHK74_003726"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFP0222471.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(11421..12278)
                     /gene="menB"
                     /locus_tag="ACHK74_003727"
     CDS             complement(11421..12278)
                     /gene="menB"
                     /locus_tag="ACHK74_003727"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFP0222472.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(12293..13051)
                     /gene="menH"
                     /locus_tag="ACHK74_003728"
     CDS             complement(12293..13051)
                     /gene="menH"
                     /locus_tag="ACHK74_003728"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFP0222473.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(13048..14718)
                     /gene="menD"
                     /locus_tag="ACHK74_003729"
     CDS             complement(13048..14718)
                     /gene="menD"
                     /locus_tag="ACHK74_003729"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFP0222474.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(14807..16102)
                     /gene="menF"
                     /locus_tag="ACHK74_003730"
     CDS             complement(14807..16102)
                     /gene="menF"
                     /locus_tag="ACHK74_003730"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFP0222475.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(16181..16486)
                     /gene="elaB"
                     /locus_tag="ACHK74_003731"
     CDS             complement(16181..16486)
                     /gene="elaB"
                     /locus_tag="ACHK74_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFP0222476.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(16541..17002)
                     /gene="elaA"
                     /locus_tag="ACHK74_003732"
     CDS             complement(16541..17002)
                     /gene="elaA"
                     /locus_tag="ACHK74_003732"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0222477.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            17067..17984
                     /gene="rbn"
                     /locus_tag="ACHK74_003733"
     CDS             17067..17984
                     /gene="rbn"
                     /locus_tag="ACHK74_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFP0222478.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            18172..19395
                     /gene="elaD"
                     /locus_tag="ACHK74_003734"
     CDS             18172..19395
                     /gene="elaD"
                     /locus_tag="ACHK74_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase ElaD"
                     /protein_id="HFP0222479.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHAATN
                     SPLKPAGPDNSDNLLFEKLSSPSKLMTIIGDEIPLISEKQSLSKVLLNDKNNELSDGT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYLPDSNSTYLEIALNDNIKIDP
                     SWPKEVQLFPINTGGHWILVSLQKTVNEKNNTQQIKCVIFNSLRALGHDKENSLKRVI
                     NSFNSELMGEMSNNKNITVHLTEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRES
                     NSDSAPYTLLKNYADEFKKYSAEEQYEIDFQHRLINRNCYLDKYGDANINHYYKNLEI
                     KHTQPKNRASGKRVS"
BASE COUNT         4794 a         5141 c         4850 g         4831 t
ORIGIN      
        1 aggaataccc ctatgcgaca acggactatt gtatgccctt tgattcaaaa tgatggtgct
       61 tatttgctgt gtaaaatggc cgacgatcgc ggcgttttcc ccggtcaatg ggcgctttcg
      121 ggtggcggcg tggagtctgg cgaacgaatt gaagaggcac tacgccgcga aattcgcgaa
      181 gaactgggag aacagctgct tttgacagaa atcacgccgt ggaccttcag cgatgatatt
      241 cgcaccaaga cgtatgcaga tggtcgcaag gaagagattt atatgattta cctgattttt
      301 gactgcgttt ctgccaaccg agaagtgaaa ataaacgaag agtttcagga ctacgcgtgg
      361 gtaaaacctg aagatctggt gcattatgat ttaaatgttg ccacccgaaa aacgttacgt
      421 ttgaaaggtc ttctttaaca atgatgacag ccatcactgg ctgtcagatt gaaaatttag
      481 tggttaacga attccccatc cagataaact ttgccttttt ccacatgcat gactaaacca
      541 tccagataat caggtttaaa tgtcgcatca cgcttatttt tagcaatata tgtcaggcaa
      601 tgattatggg tgaaaataac gatattttta tcaggcgctt tgctttgtaa gtccttaatt
      661 gcactataaa tctcattacc gcactgaaga agtcgtttat ctaccgtcag ctttttaccc
      721 gcggaaaacc aggtcgccga ctgaatggtc cggacggtat tactggaata aagatcgaaa
      781 tcagggatgt cagcgctaaa agcgttgccc agttcacggg catcctgggt acctttaacc
      841 gtaatacctg ttttatctga caagcattga ttggttgaac gatcgcaacg ttcagcatgg
      901 cgaaacaaaa cgacaaccgg gtgctgctgt gccagtctgg ccaatgtttt attgtcgata
      961 cgtggcaaac cattgctact ccaggcggca tgagtaccca gtccggcaat tgcagcgagc
     1021 gccagtaaaa tgatgaaata tttttttgac ttcaatgatg agcggcaaaa agctaacacg
     1081 taaactccac ctatagacaa gcgcaaccag acaattaccg taaaattgag cgacatttct
     1141 ggcgataatt agcagttggt gtaatattaa aaatcctacg atgtcggcaa aatgcctcaa
     1201 aattttgcca aatgcaaagc ctaaataaga aaaaatataa aaatttcaat atttacgtct
     1261 aatattagtt tcttaaggtt aagttaatat tctatcctta aaatttcgct ccaaatggca
     1321 aaatatacac aacactcttt atagcaaata taagtggaca ggtattcaat ggcggaagga
     1381 aaagcaatgt cagaattttt gcctttttcg cgaccagcaa tgggcgtgga ggaactcgct
     1441 gcagttaaag aggttctcga atccggttgg atcacaaccg gtccgaagaa tcaggcgctt
     1501 gagcaagcct tttgccagtt gacgggaaat cagcatgcca tcgcggtcag ttcagccacc
     1561 gccggaatgc atatcacgct aatggcgttg gaaattggca agggcgatga agtgattacg
     1621 ccttccctga cctgggtttc aaccctcaat atgatttcct tgttgggtgc aacgccggta
     1681 atggtggatg tcgaccgcga tacgctgatg gtcacgcctg aagcgatcga gtcagccata
     1741 acgccacgca ctaaagccat cattccggtg cattatgccg gtgcgccagt agatattgac
     1801 gccattcgcg ccattggcga acgttacggc atcgcagtta tcgaagatgc tgcccatgcc
     1861 gtcggtacgt attacaaagg gcgacatatt ggcgcaaaag gtaccgctat tttttcattt
     1921 catgccatta aaaatattac ctgtgcggaa ggtggcctga ttgtaactga taatgaaaac
     1981 cttgcccgcc agctacggat gctgaaattt cacggtctgg gtgtcgatgc ctatgacaga
     2041 caaacctggg gccgtgcacc gcaggctgaa gtcttaacac cgggctataa gtacaatctg
     2101 accgatatta acgccgcaat tgccctgaca cagttagcaa aattagagca cctcaacacc
     2161 cgtcggcgcg aaattgccca gcaatatcag caagcactgg cagctctccc ctttcagcca
     2221 ttaagccttc ccgcctggcc gcacgttcac gcctggcatc tgtttattat tcgtgtcgat
     2281 gaacaacgtt gtggtatcag tcgcgatgcg ttgatggaag cgttaaaaga aagaggcatt
     2341 ggtaccgggt tacatttccg cgccgctcac acacaaaaat attatcgcga gcgttttccc
     2401 acgctgtcgt taccgaatac cgaatggaat agcgaacgca tctgttcttt gccgctgttc
     2461 ccggatatga ctaccgccga tgccgaccgt gtcatcacag cccttcagca actcgcagga
     2521 caataagcca tgtttgaaat ccaccctgtt aagaaagtct cggtggttat tcccgtttat
     2581 aacgagcagg aaagcttacc ggaattaatc aggcgcacca ccacagcctg tgaatcgttg
     2641 gggaaagagt atgagatcct gctgattgat gacggcagta gcgataattc cgcgcatatg
     2701 ctggtcgaag cctcacaagc ggagaacagc catattgtgt ctattttgct taaccgcaat
     2761 tacgggcaac attcagcgat tatggcggga ttcagtcacg ttaccggcga cttaattatt
     2821 acccttgatg ccgatctcca gaatccgcca gaagaaatcc cccgcctggt ggcaaaagcc
     2881 gatgaaggtt acgacgtggt agggactgta cgccagaacc gccaggacag ctggtttcgt
     2941 aaaacagctt cgaagatgat taaccggctt attcagcgca ccaccggcaa agcgatgggt
     3001 gactacggtt gtatgctgcg cgcctatcgc cgtcatattg tcgatgcgat gttgcactgc
     3061 catgaacgta gcacctttat cccgattctg gcgaatatct tcgcccgccg tgccattgaa
     3121 attccagtac atcatgccga gcgtgagttt ggtgaatcca aatacagttt tatgcgcctg
     3181 attaatttga tgtacgacct ggtgacctgc cttaccacaa cgccgctacg tatgctgagt
     3241 ctgctcggca gcattattgc gattggaggt tttagcattg cggtgctgct ggtgatttta
     3301 cgcctgacct tcggaccaca atgggcggca gaaggcgtct ttatgctatt tgccgtgctg
     3361 tttactttta ttggcgctca gtttatcggc atgggattac tcggtgaata tatcggcagg
     3421 atctacaccg atgtccgcgc ccgcccccgc tattttgttc agcaagttat ccgtccatcc
     3481 agcaaggaaa atgaataatg aaaaccgtcg tttttgccta ccacgatatg ggatgcctcg
     3541 gtattgaagc tctgctggct gccggttacg aaattagcgc catttttacc cataccgata
     3601 atcccggtga aaaagccttt tatggttcgg tggctcgtct ggcagcggaa agaggcattc
     3661 cggtttatgc gacggatgac gttaatcatc cgctgtgggt ggaacgcatt gcccaactgt
     3721 cgccagatgt gattttctct ttttattatc gccatcttat ttgcgacgaa attttgcagc
     3781 tcgctcccgc aggtgcattt aatctgcatg gttcgctgtt accaaaatat cgtggtcgcg
     3841 cgccgctgaa ctgggtgctg gtcaacggtg aaacggaaac tggcgttaca ttgcaccgaa
     3901 tggtgaaacg tgccgatgcc ggggccattg tggcgcaact gcgcattgcc attgcgccag
     3961 acgatatcgc tattacgctg catcataaat tgtgccatgc cgcgcgccag ctactggaac
     4021 agacattacc cgccattaaa cacggtaata ttctggaaat cgcccagcgc gaaaacgaag
     4081 ccacctgttt tggtcgcaga acgccggatg acagtttcct tgaatggcat aaaccggcat
     4141 ccgtactgca caacatggta cgtgccgttg ccgatccgtg gccgggtgcc ttcagctatg
     4201 ttggcaatca gaaattcacc gtctggtcgt cgcgtgttca tcctcatgcc agcaaagcac
     4261 agccggggag cgtgatttct gttgcgccac tgctgattgc ctgtggcgat ggcgcgctgg
     4321 aaatcgtcac cggacaggcg ggcgacggca ttactatgca gggctcgcaa ttagcgcaga
     4381 cgctgggcct ggtgcaaggt tcacgcttga atagccagcc tgcctgcacc gcccgacgcc
     4441 gtacccgggt actcatcctc ggggtgaatg gctttattgg caaccatctg acagaacgcc
     4501 tgctgcgcga agatcattat gaagtttacg gtctggatat tggcagcgat gcgataagcc
     4561 gttttctgaa tcatccgcat tttcactttg ttgaaggcga tatcagtatt cattccgaat
     4621 ggattgagta tcatgtcaaa aaatgtgatg tcgtcttgcc gctggtggcg atagccacgc
     4681 cgattgaata tacccgcaac ccgctgcgcg tatttgaact cgattttgaa gagaatctgc
     4741 gcattatccg ctactgcgtg aagtaccgta agcgaatcat cttcccgtca acttcagaag
     4801 tttatgggat gtgtagcgat aaatacttcg atgaggacca ttctaattta atcgtcggcc
     4861 cggtgaataa accacgctgg atttattcgg tatcaaaaca attacttgat cgggtgatct
     4921 gggcctatgg cgaaaaagag ggtttacagt tcaccctctt ccgcccgttt aactggatgg
     4981 gaccgcgact ggataacctt aatgcggcac gaatcggcag ctcccgcgct attacgcaac
     5041 tcattctcaa tctggtagaa ggttcaccga ttaagctgat tgatggcgga aaacaaaaac
     5101 gctgctttac tgatattcgc gatggtatcg aggcgttata ccgcattatc gaaaatgcgg
     5161 gaaatcgctg cgacggtgaa attatcaaca ttggcaatcc tgagaacgaa gcgagcattg
     5221 aggaactggg cgagatgctg ctggcgagct tcgaaaaaca tccgctgcgc catcatttcc
     5281 caccgtttgc gggctttcgc gttgtcgaaa gtagcagcta ctacggcaaa ggatatcagg
     5341 acgtagagca tcgtaaaccg agcatccgca atgcccaccg ctgcctggac tgggagccga
     5401 aaattgatat gcaggaaacc atcgacgaaa cgctggattt cttcctgcgc accgttgatc
     5461 ttacggataa accatcatga ccaaagtagg cttacgcatt gatgtcgata cctttcgtgg
     5521 cacccgtgaa ggcgtgccgc gtctgctgga aatcttgagt aagcataata ttcaggccag
     5581 catttttttc agcgtcggcc cggacaatat gggccgccat ctctggcgac tggtgaagcc
     5641 acagtttttg tggaagatgc tgcgctcaaa tgcggcatcg ctttatggct gggatatttt
     5701 actggcaggt acggcctggc caggtaaaga gattggtcat gccaatgccg atatcattcg
     5761 tgaagcggct aaacatcacg aagtcggcct gcacgcctgg gatcaccatg cctggcaagc
     5821 ccgtagcggt aactgggatc ggcaaacaat gatcgacgat attgcacgcg gtcttcgcac
     5881 tctggaagag attatcggtc aaccggtaac ctgttctgcc gctgcgggct ggcgtgccga
     5941 ccagaaggtg atcgaagcaa aagaagcgtt ccatttgcgc tacaacagcg attgtcgtgg
     6001 ggccatgccg ttccgtccat tgctcgaatc aggaaaccct ggcactgcgc aaattccggt
     6061 gaccttaccc acctgggatg aagtgattgg tcgggatgtg aaagcagaag attttaacgg
     6121 ttggttactc aaccgcatcc tgcgagataa aggcacgccg gtttatacca ttcatgcaga
     6181 agttgaaggc tgcgcttatc agcataattt tgtggatctc ctcaaacgcg cagctcagga
     6241 aggcgtgaca ttttgccctt taagcgaact gttatcagag acgttgccgc tcggacaagt
     6301 tgttcgcgga aatattgccg gacgtgaagg ctggctgggt tgccaacaaa ttgcgggtag
     6361 tcgctgatga aatcggtacg ttaccttatc ggcctcttcg cgtttattgc ctgctattac
     6421 ctgttaccga tcagcacgcg tctgctctgg caacccgatg aaacgcgtta tgcggaaatc
     6481 agtcgagaaa tgctggcatc cggcgactgg attgtgcccc atctgttagg gctacgttat
     6541 ttcgaaaaac ccattgccgg atactggatt aacagcattg ggcaatggct atttggcgcg
     6601 aataactttg gtgtgcgggc aggcgttatc tttgcgaccc tgttaactgc cgcgctggtg
     6661 acctggttta ctctgcgctt atggcgcgat aaacgtctgg ctctactcgc cacagtaatt
     6721 tatctctcat tgtttattgt ctatgccatc ggcacttatg ccgtgctcga tccgtttatt
     6781 gcattctggc tggtggcggg aatgtgcagc ttctggctgg caatgcaggc acagacgtgg
     6841 aaaggcaaaa gcgcaggatt tttactgctg ggaatcacct gcggcatggg ggtgatgacc
     6901 aaaggttttc tcgcccttgc cgtgccggta ttaagcgtgc tgccatgggt agcgacgcaa
     6961 aaacgctgga aagatctctt tatttacggc tggctggcgg ttatcagttg cgtactgacg
     7021 gttctccctt ggggactggc gatagcgcag cgggagcctg acttctggca ctattttttc
     7081 tgggttgagc atattcaacg ctttgcactg gatgatgccc aacatagagc tccgttctgg
     7141 tactacgtgc cggtcatcat tgccggtagc ctgccgtggc tgggattact ccccggtgca
     7201 ctgtacacag gctggaaaaa ccgcaagcat tccgcaaccg tctatttgtt gagctggacg
     7261 ataatgccgc tgctgttttt ctccgtcgct aaaggtaaat tgcccaccta tattctttcc
     7321 tgctttgcat ctctggcaat gctgatggcg cattacgctt tgctggcagc aaaaaataat
     7381 cctctggcgc tgcggattaa tggctggatt aacatcgctt ttggcgtcac tggcattatt
     7441 gccacatttg tggtctcccc gtggggacca atgaacacgc cggtgtggca aaccttcgag
     7501 agctataaag tcttttgtgc ctggtcgatt ttttcgctat gggcattttt cggctggtac
     7561 accttaacaa acgtcgaaaa gacctggcct tttgccgcgc tttgcccgct ggggctggcg
     7621 ttgctggtag gattttcaat tcctgacaga gttatggaag gaaaacatcc gcaatttttt
     7681 gtcgagatga cacaagaatc acttcagcca agccgctata ttcttactga tagcgtcggt
     7741 gttgccgcag gtctggcatg gagcctgcaa cgcgatgaca tcatcatgta tcgccagaca
     7801 ggtgagttga aatacggcct taattatccg gatgcgaaag ggagatttgt cagcggtgat
     7861 gagttcgcaa actggcttaa tcaacatcgt caggagggga ttattactct cgtgctttcg
     7921 gttgaccgcg atgaagatat caacagtctc gccattccgc ccgcagatgc catcgatcgt
     7981 caggagcgtc tggtgctgat tcagtatcgt cccaaatgat ctggctaaca ttagtctttg
     8041 ccagcttgct tagcgttgcc gggcagttgt gtcagaaaca ggcaacctgc tttgtggcga
     8101 taaacaaacg gcgcaaacat atcgtgctgt ggctgggact ggcgctggct tgtcttggtc
     8161 ttgccatggt gctctggctg ctggtcttgc agaacgtacc ggtaggcatt gcctacccga
     8221 tgttaagtct gaattttgtc tgggtgacgc tggctgcagt aaaactgtgg cacgaaccgg
     8281 tatcgccgcg tcactggtgt ggggtagcgt tcattattgg tggcattgtg atcctcggga
     8341 gtacggtgta atgggcctga tgtggggatt attcagcgtc attattgcct cagttgcgca
     8401 attaagccta ggttttgcgg cgagtcatct gccgccgatg acgcacttat gggattttat
     8461 cgcggctctg ttagcttttg gcctggatgc ccggatactg ttactcgggc tgctgggtta
     8521 tctgctttcc gttttttgtt ggtataaaac gcttcataaa cttgcgctaa gcaaagccta
     8581 tgccttgctc agtatgagtt atgtgctggt atggattgct tcaatggtac ttcccggctg
     8641 ggaagggact ttttcgctga aagcactact gggagtagcc tgtattatga gcgggttgat
     8701 gctgattttc ctgcccacga caaaacaacg ttactgagtt ttccctgcca ctttacactg
     8761 ccgttcccac tcatcggggc tataactgct tgccgagagg actttcaccg tatgcagctt
     8821 ttcacgatta atacaataaa gcgcattctg caaaggcgag agtaaatccc ccactttcac
     8881 ggcgaagtca ctcttcactt cggcgatcat ttttatcgcg ccgccagcat cgcagagcat
     8941 aagtacatgt ctgttatctt gtttattgca gcacgatttt ttgaccagcc attccattgc
     9001 attatcctgt ttgctaagag ttttcacatc aatttacaga ttaacagata aactctttat
     9061 caacggcaat ggaatggcag aaatgagaag tgaaacggag ggttaattat tgctgacgtt
     9121 gcacccactc ttttagcgcc tgacgtgaaa ttttaatacc gccgtttttc agctccggcg
     9181 gtaaagtcag ccagcgcacc ggttgttgaa aacgagccag cttatctttc acccattcgc
     9241 caagatcaac ggtttgctgg tcatactcca ccaccgctac cggccgatga ccaaactcct
     9301 tgtcggcaac gggaacgata aacacctgca aaaccgcagg atgtgcagca ataacacgct
     9361 cgacttcttc cggctgaata ccctcaccgc cactgaagaa cagattgtct aaacgtccga
     9421 caatggtcag cttgccatta tgcatctcac cgcgatcgcg cgtagcgtac cagccttcgt
     9481 cattaaccag tgaaaccagt tgcccgttac gccagtaacc ttctgccata ctggcagccc
     9541 gcagccacac ttcattatta acgattttca cttcccgacc cggcagcggc gaaccaacgt
     9601 ctgccaggcc gtcggcttct ttcgcacaca ccgtggaggc aaactcggtc agaccatagc
     9661 cgcaaaagca acgaatcccc tgctcgcgcg cctgttccgt caactcgacc gggatagccg
     9721 cgccgccaag taacaccgct ttcagggaaa cggaactgcg gttaaccagc aaacgccaga
     9781 gttgtgttgg caccagtgaa gcgtgagtac agcctgccag catttgctcc aatggctgtt
     9841 tatcacgcac cgtcatccgc gcaccagcgt ataaccagcg ccacataatt ccctgaccgg
     9901 agacgtgaaa cagcggtaaa gagagcaacc agtcatcgtg atcgccaaac ggaatcaacg
     9961 ataacacccc ttcggcactg gcaagatggg cctgataagt atggacagcg gctttcggca
    10021 acccggtaga acccgaggtc aacgtcattg agcacagacg cgtcggctgc cacgtagcgg
    10081 catgtgcgcc ttcaacccgc tgaatgtgca gcgacgttaa cgccggaaac gtgttttccc
    10141 catccggcac cagcgcaaat tgcagcgtca gattgggcag caattcttca agcaacggtt
    10201 gcggcagctg agggttcacg ggcaacaccc gcgccccgca ttgcagtaac gccagccagg
    10261 cgagcagcgt ttgcggcgta ttccacgccc gcaacatcac gccgctgccc tcaacaacgc
    10321 cctgcgccgc aaagcctgag gctaattcat cgacgcgagc acaaagctcg cgccagttaa
    10381 gttgctcgtc attaagacgt aaggcgatgg cttctccccg cacttgccgc cagtgacgcc
    10441 acggccagtc agagaagatc ataacaaccg ctccagtgca tcaacttcca cgacaggcag
    10501 cgtgctaccc ggccagcgac gtacctgctg cgcctgcatc agatccagcg tgtccagccc
    10561 tggaatggtg tccggcgtta accaggcggc aatccgcgcc agttgcgtta agcctaagct
    10621 cgattcaatg gaagaactga tcaccgccgt cagccccagc gcgtgcgccg cctgtacctg
    10681 ctcgcgtact ttctccagac tgcccgtgag cgtgggtttg ataactaccg cgcgcacgcc
    10741 ctcttcagcc acaaaggcaa aatccggctc gcgcaggctt tcatcccagg caatggcaat
    10801 gccggtttca cgggcaaacg ctcgcgaatc atcgcgggtt ttgcacggct cttcgagaaa
    10861 cgcgatgcgg tggcgataat ccgggttaac atatttggca aactgctggc ctttcagcgg
    10921 tgtccaggcg cgatttgcgt caagacgcaa atgcagatcc ggaattgcct ccagcaacag
    10981 attaaccacc atgccgtcgc gcacggcttc gtacaatccc actttgacct tcgccacttt
    11041 ctcgcctggc atatcagcaa gtttgaggat cagatcgtca ggatcgccat tacacagtgg
    11101 tgccgcacgg taattggccg cctgcgggag cgtatctgcc agctccgcca atgcacagct
    11161 tacgccaaag gcaacggaag gcatctgcgg tagctcgcaa tcgcctgcca gccagttatt
    11221 tacccaggca agcagcacgc tttgcgcctc ttcccaggtt tcctggctga agcccggcag
    11281 tggggagatc tccccccacc cttcgcgctc gccctcacgc aggcaaacgt acagcccgtc
    11341 gcgggttttt aaccgcctgt cgcgcagaac aacccccgcg tccatgggga tctgccagcg
    11401 gtatacctgc gcgctacgca ttacggattc cgtttaaatt tgctgaaatc aggctgacgt
    11461 ttctggttga aagcgttgcg accttcctga ccttcttccg tcatgtagaa cagcatggtg
    11521 gcgttgcccg ccagctcctg cagccccgcc tgcccgtcac agtcggcgtt cagcgcagct
    11581 ttcaggcagc gcagcgccat cgggctgttt tgcagcattt cgcggcacca acggacggtt
    11641 tctttttcca gatccgccag cggtaccacg gtgttgacaa ggcccatatc cagcgcctgt
    11701 tttgcgtcgt actgacggca caggaaccag atttctcgcg cttttttctg cccgacgata
    11761 cgagccatgt aggaagcgcc ccagccgccg tcgaaggaac cgactttcgg gccagtctga
    11821 ccgaagatgg cattatctgc tgcgatagtc aggtcgcaca tcatgtgcag aacgtgacca
    11881 ccgccgatgg agtagccagc caccatcgcg acaaccggtt tcggacaggt acggatctga
    11941 cgctggaagt ccagcacgtt caagtgatgt acgccggaat catctttata gccgccgtaa
    12001 tcaccacgca ctttctggtc accaccggag cagaacgctt tatcgcctgc gccagtcaga
    12061 atgatcacgc cgatattgtc gtcataacgc gcatctgcca gtgcctggat catctctttt
    12121 accgtcagag gacggaaggc attgcgcacc tgcggacgat taatggtgat ttttgcgata
    12181 ccgtcggtgg atttttcata acgaatgtcc tcgaaacctt cggagcagtc gtgccattca
    12241 accggtgcgt aaagcattgc ttcatcagga taaatcatat tgtgtccttt agtcagaaac
    12301 gcaagatctg cgccagactt gcgataacgc cagcgggatt ttcccgatgc gcgttatgtc
    12361 cggcgcgagg aatgacatgg cagtcggcag ccagttccgc cgccagggcg cggaatttgc
    12421 tgtcacgttc accacataaa taataaaacg caaatgtgcg ggcgctaagg ttagcacgta
    12481 aatcaggctg gacggcgaga gaagtcgcct ccagcatggc ggcaagggtt gcgccattat
    12541 tgttgctgcg cagcgccacc agctcccggc gttgatcgtc attgagcgag gcaaaaacag
    12601 gctgttgata ccagtcggca aataccgccg ttaacggttc tgtgcgaaaa cgctgcgccc
    12661 attggcgatc ggaacgctga cgttccgcac gttgttcagc attttgcagc cccggatgcc
    12721 cgccttcgac aataacccca caaagccccg ccagcccctg gcaagccgcc atcatcgcca
    12781 cccgtccgcc aagcgagtac cccaccagcc aaaagtcaag gatgttgtaa ctaaccaagg
    12841 ttttacgcag taagtcggtg acatcatcaa atccatcgac gctaatcgcc gccgaaccac
    12901 catgacctgg gagatcaaca tacaaccgtg agtagtcggc aaacgcctcg cccacttctt
    12961 gccattcgtg gcaatcgccg gaaaaaccgt gcaaaaacac cagccagggt aaacctggtt
    13021 ttccgtgttt tgcctgcgcg tgcaggatca taaatggctt acctgcgcca gaagttgctg
    13081 gagcgtttgc gcaccatcgg tgtcgttaac caccatttca atcaccgtgg tggttggcgt
    13141 gcgccaggcg tcggcaaatg ccgtttcaag ttcctgccag ttttgcggac gatgatattt
    13201 cagctcgaac atcgcggcgg cgtgctcaaa atggacgttt tgcggcatca gatagaaacg
    13261 ctcacgctcg ctttgcggcg ttggcaacag cgagaaaatt tgcccgccgt tgttgttcac
    13321 cacaattaat accagcggcg cagaaacctg acgcaataac gccagcgcgt tgagatcgta
    13381 aagtgcggag agatcgccca caatcgccag cgtcggtttg ccgcttgccc gctgaacgcc
    13441 tgcggcggtc gaaagcagtc cgtcgatacc gctggcccct cggttgctgt acaccgggta
    13501 acctgccgga agttgcgaaa gcgcatcaat cagacgtacc accaggctgt taccgacaaa
    13561 caattgcccc tgttcaggca gatagtcgct gatgcgatgc gccagttgcg cttcgccaaa
    13621 cgcatcgcga cgggcaataa ccgcctgcat tgcctgttcc gccaggcgcg ggatttcaac
    13681 gcaccagggc tggcgttttt ctgccggatg cagctccagc cagtcggcaa tattggcaat
    13741 taagcgacgt ccgcgatggt gtgccggatc aagtcgccct tcaatgtcat caacaatcca
    13801 gtactcttct ggttcacagc ttgcctgcca ttgcaggagc cgtttgcccg tcaggctgct
    13861 tcccagttgc accacaattt gcgcctgctg cagctcgctg gtcgctttgg cattgcctaa
    13921 ccaaagatcg gcacacggca gcggctgccc ggtttgtgac agcacatcgc caatcagcgg
    13981 ccagccaaga gtttgcgccc acagggcaac ttttttgccc tcttccgcac tcatgcgccc
    14041 ggcaaccacc acgccgcgct tttgtcgcca gaagaaccag tcgcgctgtt tttcactttc
    14101 cagacgaggc gcttcacgca gccacggttt gtcgtcctgc caccagtcac ccagacgctg
    14161 ttgccagcta agcccggtat cgtccatttc gccatacagc ggttcagcaa acgggcagtt
    14221 gatatggact ccccccgcat gaagcgtacc gagagcgtgg tcgatggtag aaaccagcca
    14281 acgtgcgggg atatcctggg tcgggcgcgg caatgaaata ctgtgcgtgg ggtgagaggc
    14341 gaacattccc ggctggcgaa ttgcctgatt cgcgccgcag tcaattagct ccggcgggcg
    14401 atcggcggtt aagagaatca gtttttctcc ggttaacccg gcttcaatca gtgccggata
    14461 gagatttgcc accgccgtgc cggaggtcac aatcaccgcc accggctgct tgctgacttt
    14521 cgccagcccc agcgccagat gccccaaccc acgctcatcg aaatgggtgt ggtgaatgaa
    14581 tgcggaattc tccgccgccg ctaacgttaa cggtgtagaa cgcgagcctg gggcgataca
    14641 gatgtgtctg acgccgtgac gcgttaatgc ttccagaatg accgccgccc agcgtcggtt
    14701 aaatgcgctt actgacatga gtttgtccgg tatcaatatt gcggctaagt ataaggagct
    14761 acaaaaatag attattgata tgaatcggta atgatgcgac tcattactat tccatttgta
    14821 ataaagtacg cagccctgcc gctttgttgt cgatttcctg ccactcttgc tcggggtcgg
    14881 aaccacggac aatgcccgcg ccagcatata atcgcacgac attgccgcta atttttgctg
    14941 agcgcaggga aacgcagaat tcgctttgtt gtaatgagag atagcccgca gaaccggcgt
    15001 accattctcg ggtgaacggt tcgtgacggg cgataaactg tcgcgccaga tcgcgcggta
    15061 agccagcaac tgctgccgtt ggctgcaact gatgtaaaca gatcacatca tccgctttgt
    15121 tgagtgaagt ccagatacag cggcgaagat gctgcacttt acgcagacgc agtacctgcg
    15181 gcggtaaaac atccagcgtc tgggtatcgg cctgtaatcg ttggcagata tcttccacca
    15241 ccagcatgtt ctcgcgctgg tttttatcat ccgccatcag ccactctcct aactgctgcg
    15301 cctgcttatc atcaggatta tttgctactg ttcccgccag cgcttcagta cgcagcgctt
    15361 tgtcacgccg ccgccataac cgttccggtg aagagccaag aaaagcattt tcgccatcaa
    15421 aggccatgta aaaatggtag caattcagat tcagtcgacg actggcagcc atcatcgccg
    15481 ccgcgttgac cggacttgcg aaatgcaggt cagttgcccg agcgagcacc actttgtcga
    15541 gctcaccttc ggcgatggtt ttcgttgcca gttcgattaa ttgcgtccag cccgttttgt
    15601 ccggccagtg ttgttctcgc gtggtggtta aatgtaaccc aggcaagggc ttgatactca
    15661 ccagtgtggc gataaattct tttgcctgaa tcgcatcgtg ctgaagggag ctttcgctga
    15721 atagcgtcag ccgcagcgtg gctttaccgc cacagcgtcg ccattccagg cggggtaaaa
    15781 gtaaattgcc ctgcgacggg tcaaaagcat tcagccccca aatgcgtaag tcggcgtgtt
    15841 ccgggtgctg gcgaagaaaa cgttgtgcct ggtccaacga cgtaaaacgg gtaatcgcgc
    15901 ccaggacggc agcttcttca tcaccattac gttgttgcca gtagaattgc gggtacgttt
    15961 gctgactggc cagccagctc aaggcatcaa aagcgtcttt gagagggaaa ggaatatcga
    16021 taacccgaat gccgggtgtc gccggaatct cttgcgacaa atggcgcagt agattttcca
    16081 gcgccgtagt aagtgattgc acgcggacct ctccctgtta aaagcctcac attatacggg
    16141 gtactacaaa aaaaatgcag taccccggtg tagggaggtt ttaacggcgt gccagcaaca
    16201 gtcctagtac cagcccaacg gccgcgccca caccaattcc ttgccagggt ttttcgtgga
    16261 cgtagtcatc agcacgataa actgcctgct tcgcccgata gtaataactg tctgaagcct
    16321 ggctaacccg ttttttcaca tcatccagcg ctttttctgc acgcgctttc agctcaacat
    16381 atttctgatc ggcgggatcg ccagaggagc ggagcacctc ttccagtgtt tcactaagca
    16441 gcgtcaggtc gtcatcgata cgtgtatcac caaactgatt agacattctc gttctccatt
    16501 tgcgtaaaac ctgtcactaa ctatagacaa gggttgttga ttacgcctga attacctcgc
    16561 gtgccatccc aatgtgtggg ataccatctt cttcatagac ctccgtcaca gggataaaac
    16621 cgaaactctg gtagaagttt tgcagatgcg cctgcgcccc gaggtaaaca ggcttatcag
    16681 gccagtgatg cgtacatgtt tcgagtgttt tactcatcag ctgctgcccc actttttcgc
    16741 cacgcaacgc ttcactgaca atcacccgac ctataacgac cggctcaaga tcatcatcac
    16801 ttttcagaat cctcgcatat gccaccagtt catcattttt ccaaccgaga atatggcgat
    16861 tatcccccgt caaatcatca ccatcgatat cctgataagg gcaattctgc tcgacaacaa
    16921 acaccgcgca acgcagttgt aataaggcat aaagctggga aaccgataat tcagagtgat
    16981 gcagatcttg ccattcaatc ataatgctct ccttttagat gtatgcctta tactagccgg
    17041 aatatttttt gaaacgtgat gaactcatgg aattaatttt tttaggtact tcggccggtg
    17101 tgccaacccg cacgcgcaat gtcacggcaa tattgcttaa cctgcaacat ccgacccaga
    17161 gcggtctttg gttgtttgac tgcggtgaag gcacccagca tcagctactg cataccgcct
    17221 ttaaccctgg aaaactggac aagattttta tcagtcacct tcatggcgat catctttttg
    17281 gtttacccgg cttgctgtgc agtcgttcta tgtcaggcat tatccaaccc ttaacgattt
    17341 atggtccgca aggtatccgt gaatttgtgg aaaccgcgct gcggattagc ggctcatgga
    17401 ccgattatcc gctggaaatt gtcgaaattg gcgctggcga aattttcgat gatggcctgc
    17461 gcaaagtgac cgcttatccg ctggaacacc cactggaatg ttatggctat cgtattgaag
    17521 aacatgataa accgggtgca ttaaatgccc aggcattaaa agctgctggc gtgccgcctg
    17581 gcccactgtt tcaggaatta aaagcgggca aaacaatcac gttggaagat ggaaggcaga
    17641 ttaacggcgc agattactta gctgctccag tgccaggtaa agcgctcgct attttcggcg
    17701 ataccggccc ctgcgatgcc gcacttgacc tggctaaagg tgtcgatgtc atggtgcacg
    17761 aagcgacgct ggatataacc atggaagcca aagccaatag tcgcggccat agctctacac
    17821 gccaggctgc gacactggcc cgtgaggctg gagtcggtaa gctaatcatt acccacgtca
    17881 gctcgcgcta tgatgacaaa ggttgtcagc acctgttacg tgaatgcagg tcaattttcc
    17941 cggcgactga actggcgaat gatttcaccg tgtttaacgt ttaactctgc ttattattaa
    18001 aaacagggcg aaacttgccc tgttatcgca acccgcgcta catatatcca cccacacggg
    18061 catattgagc gatttttgat atttattcat ctcctcacgc actatattca gagcaataag
    18121 ttgcctctat ttatcttgat tcagcacaac ctttgcaact aaaaggagcc catgatggtt
    18181 acagttgtca gcaattattg tcaattatct caaacgcaac tcagtcagac atttgcagaa
    18241 aaatttactg tgaccgagga attactgcag tctttaaaaa aaacagcgtt atccggagat
    18301 gaagaaagca ttgagttact gcataatatt gcgttaggct atgatgaatt tggaaaaaaa
    18361 gctgaagata ttctttacca tattgttaga aacccaacaa atgaaaccct atcgattatc
    18421 cgacttatca aaaatgcctg tttaaaatta tataatttgg cacatgccgc aaccaactcc
    18481 ccccttaaac cagctggtcc ggataattca gataatctcc tgttcgaaaa gctatcctcc
    18541 ccgtcaaaat taatgacaat tatcggtgat gaaattcctc ttatatcaga aaaacagtcg
    18601 ctttcaaagg tgcttttaaa tgataagaat aatgaactga gtgatggtac aaacttctgg
    18661 gataaaaatc gtcaattaac cacagatgaa atagcttgct atcttcagaa gatcgccgct
    18721 aatgcaaaaa atactcaagt caattatcct actggtctct acctccccga ttccaacagc
    18781 acttacctgg aaatcgctct caatgataat attaagatcg atccatcatg gccgaaagaa
    18841 gtccagttat tccccataaa tactggcgga cactggatat tagtttcgct acagaaaaca
    18901 gttaatgaaa aaaataacac acaacaaata aaatgcgtca tattcaactc attgcgtgca
    18961 ctaggccatg ataaagaaaa ttcacttaag cgtgtcatta atagttttaa ttctgaactc
    19021 atgggagaaa tgtcgaataa taagaatata acagttcatt taaccgaacc agagataata
    19081 tttttacatg ccgatcttca gcaatactta agccaaagtt gcggtgcatt tgtgtgcatg
    19141 gcagcccagg aagtgattga acaaagggaa agcaattctg acagcgcccc ctatacgtta
    19201 ttaaaaaact atgctgacga atttaaaaaa tattcagcag aagagcagta cgaaattgat
    19261 tttcaacatc gactgataaa cagaaattgt tatttagata aatatggcga tgctaatatc
    19321 aatcattatt ataaaaactt agaaataaaa cacacacaac ccaaaaatag agcgtcagga
    19381 aaaagagtaa gttaacatca ttgttaatta attcgaccag tcagtaaata tgctgactgg
    19441 taggacatag tttgctaaaa agtcatcact gactgatgcc agtcacacca tccgccagtt
    19501 caatcttcgg gtatttccag cgtatctcgg cacgcgattg ctggtctgtt gatgtagtgg
    19561 attgttgttg ctgacttttc ttaccatcag atttctgccc cctgtcaact caatct
//
LOCUS       DAZGSP010000093        19372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZGSP010000093 DAZGSP010000000
VERSION     DAZGSP010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.093"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..857
                     /locus_tag="ACHK74_003735"
     CDS             <1..857
                     /locus_tag="ACHK74_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HFP0222480.1"
                     /translation="ATPTTAVSSQSTALPPRPLLQATQLPALVMTGKPGQSSVVYLRE
                     ARQRSVSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHRALNTLLTGQQLYG
                     HMDWNRKILTVTTTAAPPVDLTAPQGSQKTADTPRNPFRSTTGTAADQAQVPPAVSAS
                     RAQSTTGTSGTSAPRTTTPAPVVTKPVAPPQKVTPPRQWKMEKGTTLKDGLARWTSSE
                     KCPHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPVEKPLYARTSTAQCLL
                     RVTDRPQE"
     gene            862..1299
                     /gene="pilM"
                     /locus_tag="ACHK74_003736"
     CDS             862..1299
                     /gene="pilM"
                     /locus_tag="ACHK74_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFP0222481.1"
                     /translation="MGWFIVFIMVITTTGNFWFTSQLSRSEHRHQAAENTQQAATFIR
                     YMNAINDYLHQHPERRTAGGRLTSTQLGIPGTDTVSHIISQQRVFVWATETPGLMAAL
                     REQCNDSALLGRVENGRLLDTAGRALSVTLPSVIPDHVILWMN"
     gene            1330..2949
                     /locus_tag="ACHK74_003737"
     CDS             1330..2949
                     /locus_tag="ACHK74_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFP0222482.1"
                     /translation="MNKKILAIMIAAALLQGCASYRQLESTRKEAVAEQQRAREMMSD
                     LRTRTPVVKESSRQWINPRPVMDKNTGKTVPGCPIVIHTRQSITLQQVVQRITESCHI
                     PVRITPDVLAYLNTSTTGSTQQIQGALPPPDASGMVPLAAIGASDTPPSRLPGNTAAL
                     SELNASGLTTLLNAVSSRLGISWRYDGSHIIFYYLETQTFPITYMDSQVAYNATVVSG
                     TMSSNGSSGGSASDAISGDASNTQTTTVDMKSALYQDVKNAVTSMLTPGIGRMFMSTG
                     FITVTDNPQVLETVRAFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDAITGGVSIVDGKLTGSKAFLKALSSQGDVSVVTQNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            2970..4265
                     /gene="pilO2"
                     /locus_tag="ACHK74_003738"
     CDS             2970..4265
                     /gene="pilO2"
                     /locus_tag="ACHK74_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFP0222483.1"
                     /translation="MTDISPTERQMFPLTAGRNVFLAGLEWKTLPSQYRHARDFARAQ
                     KADLFLACQYLSNEDANTHTMVATASRRILPGKPRQYLSLALLILPLLEQGGYAITEL
                     TLPGETPRYSFVSAVDGVLVSDLVGSGEEVREARDTFLSINTEPEQGWTRYEPVAFSA
                     GDQNQALPLSTLTGAGKHPAAARLNPVSRGAQFITVSLIIALLGAAWYGWQYYERWKT
                     EQAAQAAAARQVTEEQISPPWPGLPETDAFIQGCADIWTSLPLSAAGWRYSLAECSTD
                     GGSGNLRASYTNTAGATVTDFIRRISDMTGTRPFIVMPEGKTGGIGLPVSFRLPEHPV
                     DVDALPESSVLQEQLTTLAQSMPLTLDWQEVSNSVTDENGNIIQPPWKEYDLQIQTLL
                     PAYQLVERLKAPSVRFISVTRQLENGRFHYQFTGKYYAR"
     gene            4255..4707
                     /gene="pilP"
                     /locus_tag="ACHK74_003739"
     CDS             4255..4707
                     /gene="pilP"
                     /locus_tag="ACHK74_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFP0222484.1"
                     /translation="MHAKTKALLVSALLLPATAMATTGNAEEQSPRQLQIEQIQDETA
                     LYEAMLAREKIRHELRMLEQGIPAEPGRPQESTSAIPTAPSTAASPRLTEIFGVQPQL
                     SARIELSGGHITDVKTGQNIPGTPYLVEKITPDSVVLEQSGIRKTLRP"
     gene            4756..5310
                     /locus_tag="ACHK74_003740"
     CDS             4756..5310
                     /locus_tag="ACHK74_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222485.1"
                     /translation="MNISELISWLSLIIRDLETAAAEYGVNHTDIVHEATQLQEQLCR
                     GKQVTPAQLRALSARLWGARMRLAAQYGQDAPLMNDLAFLSNCLKYDADRLNDRWRYR
                     EWISAAESFVLPLVFIIPLLIVLCYMMKSGNSGGAELCAALAGAWCTGLTFLCLWAKD
                     PVGLFWSLYSFIPLYFLWCDISPA"
     gene            5406..6002
                     /locus_tag="ACHK74_003741"
     CDS             5406..6002
                     /locus_tag="ACHK74_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222486.1"
                     /translation="MLENKTTDDFIFVKQHIYRIFQWMHETPLINNGNTPVTDRLIAD
                     VRWMLEHHYYPSPGQVDRIISVLRETKLQLLREKTDRQELVEEIQQKLNTLKDIRHFL
                     KLNTNYKQDGDIISLTPSGEWKNTSIILSVVAVMLMIFASICNLSGITPYFIYTAGII
                     FYALATPVLTLRSTQPEKNIPLLFAFGGVIIFTLLSLC"
     gene            6099..7607
                     /locus_tag="ACHK74_003742"
     CDS             6099..7607
                     /locus_tag="ACHK74_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFP0222487.1"
                     /translation="MLESADLPPDDILDYVAIDTDKTGHLRVRVVEGKKHLRAVQEYL
                     TRLRARHQGRVGDFEFTTLDVIARLRQDTTTAGDESVINPVQQKMLGYIRHSASLGAS
                     DLHMTPGRDNTDFTYVEARVHGELEVLDILRKEEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLSEQYLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQQIPTFSMLGYHPDQE
                     RTLRRILQRPEGIITLSGPTGSGKSTTLRTASAAYLEQYGFNNTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTAQGWVDSIKSALRLDPDGILNGEIRDHDSAITAIKAAMTG
                     HLMLTTIHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLICPHCRRPWHEV
                     ATERTDDERRLVESVCNPDQVYLRNHEGCPHCWRGVTGRTVIAEVISPDARFFQLYRE
                     KGRIEARTYWHRELGGMTRNQHLLGKINSGQVDPLAAHYISPIDEDSYTLLH"
     gene            7609..8709
                     /locus_tag="ACHK74_003743"
     CDS             7609..8709
                     /locus_tag="ACHK74_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFP0222488.1"
                     /translation="MRFLEKLNNTDIELTALTVWIARKTFALNRVEFYEALGGMIRDG
                     TPSLRALEFLCEIETDFGEKKGTSGMYFLAKECIDSIKASGMLSGALLTWVPPEEATL
                     IRNGEERGNIADALFQVARTVKARREMTSSLIAVCLYPLLLLSLCVVNMYNAHTRIIP
                     IVSAFASEDKWSFQMKLLAGMSEFFIGYGFWLCGTIFALTLLIRFSFSRYTGPGRRML
                     DKAPPWSLYQTLHGVNYLFSISSMLQINIPLSQALLRMEKGAEKNKWLKERIQAIRKH
                     VLSGQNLATAMRNSGYDFPSRICINKLLLNSEREDSVATMALYADQWLEEAKKRVKRI
                     GLIITGISGGFVFFFILNMISAIYSISDMLKQ"
     gene            8757..9293
                     /locus_tag="ACHK74_003744"
     CDS             8757..9293
                     /locus_tag="ACHK74_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFP0222489.1"
                     /translation="MNFRSCLYSYFALKKEKEEDKGLTLLEIAGALIIIGIVVALAMS
                     QMSSTMSSSDNQRELKNLQTIATKMKQYKFQGRYTGTDYIKTLTESGSLPADMIAGSN
                     KAKNAWGGDVTIAATSNKYGYTITSKAVPKENCVELINSLRSSSMFTKIKGQTPASVD
                     PVTVCSAATNDITLETSS"
     gene            9341..9823
                     /locus_tag="ACHK74_003745"
     CDS             9341..9823
                     /locus_tag="ACHK74_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFP0222490.1"
                     /translation="MKWLFFSAVICPLFFALPAEAVATNTLLDSCFIQAGKRYQIAPD
                     LLKTIAQQESSLVATAINHNKNKSGKIISTDYGIMQINSVHIPELKKLGVIKDKNDLL
                     NNPCLNIQTGAWILARHFQRCGVNWECLGSYNAGFSKNNTARRMIYARKIHNRYFRQY
                     "
     gene            9783..10490
                     /locus_tag="ACHK74_003746"
     CDS             9783..10490
                     /locus_tag="ACHK74_003746"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFP0222491.1"
                     /translation="MPEKSTTGISDNINKYEVTVSALLSMTIIIIILAVILYLAYSTA
                     ALYLSAVHDTRPPRPVVYVCCLLAIVSVSLNGAYGVEATGLLFLSILTGLLTVMTLTD
                     IAVCRLPRIFTLSLIVLGAAFRYSLEELTYSLLNASLWFGMTYLLRQFFITAKGTEAL
                     GLGDVFLIAGIAMWTQPQHTPLLITAAASGAFLFILLFCRRRRQQALPFAPFLCASLY
                     ALTLLPDSVFRTSEIFT"
     gene            10487..11728
                     /gene="pilV"
                     /locus_tag="ACHK74_003747"
     CDS             10487..11728
                     /gene="pilV"
                     /locus_tag="ACHK74_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFP0222492.1"
                     /translation="MKKFRHDDDKGFILLEILAGLIVIGIATPLIYSEIENWLNEQLY
                     QSAASHADAYNTAARNYIADNNATLHNGSLPANFTADDLIRKGYLKQGFNRSPFGQSY
                     ITGIRRNQTTGRLEALTCSTGGQNIKEDGLRSVAGQLPGLGGYIGKNGTATGAFGAWT
                     DKPGDYGLTCSAGHIAVVMTGDDLQESDRLYRFQVPGRPELNQMHTAINMGGNDINNA
                     GNLNGQKATVKGDITSEDGWLITRNNKGWMNTTHGGGFTMTDSQWIRAVNNKGITTDG
                     EIKGGKVSGGTIRSDGRLSTGEYLQLEKTATAGTSCSPDGLVGRTSTGAILSCQSGVW
                     KNNISDALVFDYSACYDAEEDSRKPVYANWGSGSNGFAVCDGKAGYGAAGFRAYRGKP
                     GYSGSMVWVKIRCCKLKVNTP"
     gene            complement(11734..12135)
                     /locus_tag="ACHK74_003748"
                     /pseudo
     CDS             complement(11734..12135)
                     /locus_tag="ACHK74_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            12187..13356
                     /locus_tag="ACHK74_003749"
     CDS             12187..13356
                     /locus_tag="ACHK74_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP0222493.1"
                     /translation="MFQKNKTRKMSLSRALDKYLKTVSVHKKGHLQEFYRVNVIKRHP
                     MADRYMDEITTIDIAGYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGT
                     CRMNPVELVRKPKISNGRDRRLTSGEERRISRYFRDKNPQLYVIFHLALETAMRQGEI
                     LSLGWEHLDLQHGVAHLPETKNGSPRDVPLSRKARNYLQILPQQINGNVFSYTSSGFK
                     SAWRTALLDLKIENLHFHDLRHEAISRLFELGTLNVMEIAAISGHRSLNMLKRYTHLR
                     AYHLVSKLDARRKQTSKISPYFVPYPATVRNRNGVFIVTLHDFDLESRAETRELAISH
                     AGVLLLRALAQAAQRGERVPTPGELPANIDERVMICPLTNSQFMTKANSIFISSS"
     gene            13398..13688
                     /locus_tag="ACHK74_003750"
     CDS             13398..13688
                     /locus_tag="ACHK74_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222494.1"
                     /translation="MKHLSSRAATADNNRIVTIEDVYTIAFSQPIHTESALGQRWMLI
                     HTGEYESASDAKRFLDTLVTGHCACMVTPSPVLDHDTWLRIRLLMGEIRDAK"
     gene            complement(14118..14453)
                     /locus_tag="ACHK74_003751"
     CDS             complement(14118..14453)
                     /locus_tag="ACHK74_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222495.1"
                     /translation="MKIKQHKEKLEIIRNIIRETLLGNAAIVVLVSMIFVMLEKPWSV
                     MSVLCIFVYCISLMIVSLWWFFIVFIYISELTESVNIMRVLVAIWVLSSESFIIYNII
                     GIFHKVFMY"
     gene            14715..15536
                     /locus_tag="ACHK74_003752"
     CDS             14715..15536
                     /locus_tag="ACHK74_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFP0222496.1"
                     /translation="MKPDSTGIATRMMLSLDRERICECLLSNRQLQSTPLQVRYPQGV
                     RDALGIMSEQLSLSVSDLTRILVEEALSEMFLPADNIVRRLLCRMEHIMQAHDISATT
                     MAALLTPWNIRPAVFREPDRLTDYLTGDILAALADWFYLSPEWLNGRVHYPLYRPGDW
                     PVTQEAFCRIISSGENVDIILWHGFPFAGTHSGEYCGVLLRQKKEINNAIIYPVLSLY
                     PVRMDMEKEGWFQMVRKISPDIPVRAVTLTPAQAEYLITGKILPTVLFRVSLSPW"
     gene            15640..16842
                     /locus_tag="ACHK74_003753"
     CDS             15640..16842
                     /locus_tag="ACHK74_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFP0222497.1"
                     /translation="MMMKKHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKAGPEDDFSLLLPSVGGQLSDPGNISDNADKISDDWKAFDRAID
                     SQSNVSQTAARLKTRLQDFRHTHADAQVGTSAVATLPGDTLAAALMLKSYGTVSVDGK
                     VSDADLNYLESIANSNGQDVDKDRLTSQAFARAALVTDVGIALATELETAGQKWSLGF
                     TPKFQRVDLFNYNVLVKNYDSSAFKGDRYHNTRNGINADIGASMDLDDNWTLGLVAQN
                     LLPRSIETKEVNGVTETFRIRPQVTAGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AIGAEFNAWKWAQLRAGYRQNLASNDGCAFTAGLGISPFDVVHIDVAGIAGTDNNYGA
                     VAQLQFTF"
     gene            16982..17152
                     /locus_tag="ACHK74_003754"
     CDS             16982..17152
                     /locus_tag="ACHK74_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222498.1"
                     /translation="MFNKNKTHIENKAFMVEPFRDPTANECNVVIANDICIRVALSPS
                     EVLQPFSKNTNQ"
     gene            17201..17653
                     /locus_tag="ACHK74_003755"
     CDS             17201..17653
                     /locus_tag="ACHK74_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081199.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HFP0222499.1"
                     /translation="MNRMIPAGCLAVLCLNGCHLPPDNTAPTRKYGAASDVAVARHAQ
                     YQLWGQGIYSAALPERQSGGSLKAHSDRVSFDWEGDALELLNELARLRGQQFSYSGVR
                     LPLPVSLHVRDMTFADVLRLTEAQTAWRATLSQSAGRLHLSFMTPEKK"
     gene            17650..18486
                     /locus_tag="ACHK74_003756"
     CDS             17650..18486
                     /locus_tag="ACHK74_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFP0222500.1"
                     /translation="MKTHLCLLLSALLLTGCAGQHHRSSAVMTENAPPPDIRAWLTPS
                     GNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISK
                     EGWLPPVIDEAQDVAHIRPDQIRTASRVWTILRPERFVSNPPGWRDWLLKGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRLNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQMQTVTGNGQKLITGDRVRRLKTHAEFTLQKSRWRPLVSTEGEKNDI
                     TR"
     gene            18470..>19372
                     /gene="traJ"
                     /locus_tag="ACHK74_003757"
     CDS             18470..>19372
                     /gene="traJ"
                     /locus_tag="ACHK74_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFP0222501.1"
                     /translation="MISPDDFGTFPFSRFTAEEFRHFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSVNPLPSSIMMALSDELFGREVLPRVLSGHPVDRTVQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKAISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRHILKNFPDRKIVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFIL"
BASE COUNT         4934 a         5323 c         4743 g         4372 t
ORIGIN      
        1 cagcgacgcc cacaaccgca gtcagcagcc agagtaccgc actgcctccc cgcccgctcc
       61 ttcaggccac acagctgccc gcactggtta tgaccggtaa acccggtcag tcatccgtcg
      121 tttatttacg tgaggcacgt cagcgctccg tcagtgactg gatacgccag ttgctgccac
      181 ccggctggca gttccggcaa cagaacgctg ccataccggc tctgaacaaa cgcctccccg
      241 tctggtccgt taatgaccag tggcaccggg cactgaatac tctgctgacc gggcagcaac
      301 tgtacggaca tatggactgg aacaggaaaa tactgactgt cacgaccact gcagcaccgc
      361 ctgttgacct cacggcaccg cagggttcac agaaaacagc ggacacacca cgtaatccgt
      421 tcaggagcac aaccggaacg gctgcagacc aggcacaggt accacctgcc gtcagcgctt
      481 cccgggctca gagcacaaca gggacatccg gtacatctgc gccccgcaca accacgcctg
      541 ccccggtggt cacgaaaccg gtagcaccac cacagaaggt cacaccgcca cggcagtgga
      601 aaatggaaaa agggaccacg ctgaaggacg gactggcccg ctggacgtcg tccgaaaaat
      661 gcccccacgg cacctggacg ctgcgctggg aaacgcccgt taactacagt atcgatgccc
      721 ctctggtttt caccggcagt ttccgccagg cgctggaaca gctcttcgca ctctacaccc
      781 cggtggaaaa accgctgtat gcccggacca gcacagcaca atgtctgctg cgggtgacag
      841 atcgtccaca ggaataacct tatgggctgg ttcatcgtgt tcatcatggt catcacgacc
      901 acaggcaatt tctggttcac cagtcagctc tcccgcagcg aacaccgtca tcaggccgca
      961 gaaaatacac aacaggcagc aacgttcatt cgttatatga acgccatcaa tgattacctg
     1021 catcagcatc cggaacgacg gacagccgga gggcggctga catccactca gctcgggatt
     1081 cccgggacgg ataccgtcag tcatatcatc agccagcaac gtgtttttgt ctgggctaca
     1141 gaaaccccgg gcctgatggc cgcgctcagg gaacagtgta atgactctgc tctgctcggc
     1201 agggtggaaa acggccgact gctggacact gccggcagag cgctttccgt cacacttccg
     1261 tcagtgatcc ccgatcacgt catcctctgg atgaattagt cacgaatctg aataaatcac
     1321 ggatctccca tgaataaaaa aattcttgcc attatgattg ccgccgctct gttacagggc
     1381 tgtgccagtt acagacagct tgagagcaca agaaaggaag ccgttgcgga acaacaacgg
     1441 gcacgggaaa tgatgtctga tctgcgaacc cggacgccgg ttgttaagga atcctcacga
     1501 caatggatca atccccgtcc ggtcatggat aaaaacaccg gcaaaaccgt tcccggttgt
     1561 cccattgtca ttcatacccg tcagagcatc acccttcagc aggtggttca gcggatcacg
     1621 gaaagctgcc atattccggt acggatcaca cctgatgtac tggcatacct gaatacatcc
     1681 acaaccggca gcacacaaca aatccagggc gcgttacccc caccggatgc gagcggcatg
     1741 gttccccttg ccgccattgg cgcatctgac acgccaccat cccgtctgcc gggaaatacc
     1801 gcagcactgt ctgaactgaa tgcctccggc ctgacgaccc tgctgaatgc ggtctcctcc
     1861 cgtctcggta tatcctggcg ctatgacggc tcgcatatca tcttctatta tctcgagaca
     1921 cagaccttcc ccatcaccta tatggacagt caggtggcct ataacgctac tgtggtcagc
     1981 ggcaccatgt cctcaaacgg ctcatcagga ggcagtgcat cagatgccat ttccggtgat
     2041 gccagcaata cccagaccac cacagtggat atgaagtctg cgctgtatca ggatgtgaaa
     2101 aatgcggtca catcaatgct gaccccgggt atcggacgca tgttcatgtc cacgggcttt
     2161 atcaccgtca cagataatcc acaggttctg gaaacggtcc gggcattcat tgaaaaacgc
     2221 aatgaagaga tgaaacgtca ggtggtgctg aatgtggaaa ttctgagtat ccggaaaacc
     2281 cgcaatgagc aggccggtat cgactggaac gcagtcttca gcgacagaac gctgggactg
     2341 agtctcggca gcacattcac cagtgcagcc agtgatgcca tcacaggtgg tgtttccatt
     2401 gtggacggta aactcaccgg ttcaaaagcc ttcctgaaag cattgtcttc ccagggggat
     2461 gtcagtgtgg tgacccagaa ctctgccgtc accaaaaacc tgacgccggt gccaatgcag
     2521 atcgccaatc agcagagtta catagagagt gtgacgacgg ataccactgc caatgtcggc
     2581 tcatccacct ccctgaatgc ggccaccatc accaccggtt ttaacatgac ccttctgccg
     2641 ttcattctcc cggactcaca gacactgcag ttgctctatt ccatgagtct ctccgacaaa
     2701 ccggttattg agaattatga gagcggtgga agtaaagccc agctgccgaa tgtggatctg
     2761 aaaaccatca atcagaccgt ggatctgaaa tccgggcaga cggtcattat cagtggcttc
     2821 cagcagtcag gacgtcgcag cggaaaacag ggtgtaggca cgccgggatt cttcggtctt
     2881 ggcggtggga tcaacagtga aaacgacgac accattctcg tggtgctgat cacgcccaat
     2941 atcatctgat ttgcacaagg aggacgatcg tgacggatat ttcaccgaca gagcggcaga
     3001 tgtttccgct gacggcaggg cgcaatgtgt ttctggccgg tctggaatgg aaaacactgc
     3061 cctcacagta ccggcatgcc cgcgactttg cccgggcaca gaaagccgac cttttcctgg
     3121 cctgtcagta tctcagtaat gaagatgcca acacccacac catggtggcc acagccagtc
     3181 gccgcatact gcccggaaaa ccccgtcagt acctctccct cgccctgctg atcctgccac
     3241 tgcttgaaca gggcggctat gccatcacgg aactgacgtt acccggagaa acaccgcgct
     3301 acagctttgt ttccgctgtc gacggtgttc tcgtcagtga cctggtggga agcggagaag
     3361 aggtccgtga agcccgggat acctttcttt ccatcaacac cgaaccggag cagggctgga
     3421 cacgctatga accggtggca ttttcagcag gcgatcagaa tcaggccctg ccgctgtcca
     3481 ccctgaccgg cgctggtaaa catccggctg ccgcgcgcct gaatccggta tcccggggag
     3541 cgcagttcat cactgtctcg ctgatcatcg cccttctcgg cgctgcatgg tacggctggc
     3601 agtattatga acggtggaag actgaacaag ccgcgcaggc agcagctgcc cgacaggtca
     3661 cagaggaaca aatcagtccc ccctggcccg gactccccga aaccgacgca tttatccagg
     3721 gatgtgctga catatggacg tcactgccac tgtctgccgc cggatggcgt tattcgctgg
     3781 ctgaatgttc aactgacggc ggcagcggca atcttcgcgc ctcgtacact aatacagctg
     3841 gcgcgacagt gactgacttc atccggcgca tcagtgatat gacgggcacc cgccccttta
     3901 ttgtgatgcc ggaaggaaag accggcggca ttggcctgcc agtcagcttc cgcctgcctg
     3961 aacatcccgt cgacgttgat gctctgccgg aaagctctgt cctgcaggaa caactgacca
     4021 cgcttgctca gtcaatgcca ctgacgctgg actggcagga ggtcagtaac agtgtcaccg
     4081 atgaaaacgg aaatattatc cagcctccgt ggaaagagta tgaccttcag atacagacgc
     4141 tactcccggc ataccagctg gttgaacgtc tgaaggcgcc ttctgtccgt tttatctctg
     4201 tcacacgaca actggagaac ggtcgtttcc actatcaatt tacaggtaaa tattatgcac
     4261 gctaaaacaa aagcccttct ggtttcagcg ctgctgttac cggcaacggc aatggccaca
     4321 accggaaacg cagaggaaca gagtccccgg caactgcaga ttgagcagat acaggacgaa
     4381 acagccctgt atgaagccat gctggcgaga gagaaaatcc gtcatgagct gcgcatgctg
     4441 gagcagggta ttcccgcaga gcccggtcgt ccgcaggaaa gcacgtccgc catcccgact
     4501 gccccgtcga cggcggcatc cccccgtctg acagaaatat ttggtgtaca gcctcagctg
     4561 agcgcccgga tagaactttc gggtggacac atcaccgatg tgaagacggg ccagaacatc
     4621 cccggaaccc cttatctcgt ggagaaaatc acaccggaca gtgtggtact ggagcagtcc
     4681 ggcatcagaa aaaccctgcg accatgaaac cggccgccag tacaggcggc tgtccgtaca
     4741 aaaggatgaa cgacaatgaa catcagtgaa ctgatatcct ggctcagcct gatcatccgt
     4801 gacctggaaa cagccgctgc agaatacggg gtgaaccaca cagatattgt ccacgaggca
     4861 acacaattac aggaacaact ttgtcgggga aaacaggtca ccccggcaca actccgtgcc
     4921 ctgagtgccc ggttgtgggg tgccagaatg cgcctcgcgg cgcagtatgg ccaggacgcc
     4981 cccctcatga acgatctggc gttcctgagc aactgtctga aatatgatgc cgacagatta
     5041 aacgaccgct ggcgttaccg ggaatggata tccgcagccg aatcctttgt cctgccgctc
     5101 gtcttcatta tcccactgct gatcgtgctc tgttatatga tgaagagcgg caattccgga
     5161 ggcgctgaat tatgcgcggc acttgccggc gcatggtgca ccggactgac attcttatgc
     5221 ctgtgggcaa aagatcccgt cgggcttttc tggtcactct acagcttcat tccgctgtat
     5281 ttcctctggt gtgatatatc acctgcgtaa gcgattacag tgcagaaata atattccgta
     5341 caatacatca caaatcacct cacacccggg acgttttatt attaacactc gacgggatat
     5401 atctcatgct ggagaataaa accaccgatg attttatttt tgttaagcaa catatctacc
     5461 gcatatttca gtggatgcac gaaacacctc tcattaacaa cggtaataca ccggtaactg
     5521 accggttaat cgccgatgtc cggtggatgc tcgaacatca ttactatccc tcccccggcc
     5581 aggtggaccg tattatatct gtactgaggg agacaaaatt acagctgctc cgtgaaaaaa
     5641 ccgacagaca ggaacttgtg gaagagatcc agcagaaact gaacacgctg aaagatatcc
     5701 gtcatttcct gaagctgaac acgaactata aacaggatgg cgatatcatt tcacttacac
     5761 catctggtga atggaagaat acttccatta ttttatcggt agttgccgtc atgcttatga
     5821 tattcgccag tatatgtaat ctgtccggca tcacacctta ttttatttat accgcaggta
     5881 ttatttttta cgctctcgcc acaccggtat taacgctgag atcaacacag ccggagaaaa
     5941 acattcccct gttattcgcc tttggaggcg taatcatttt tacactgtta agcctgtgct
     6001 gaaaatgtcc agattctctc cgcagaagaa ctgattattt ccctcgctta ataacagaaa
     6061 acatattttc cgggcaccgc tgcccgtaag gaggccttat gttggagtct gcagacctgc
     6121 caccggatga cattctggat tatgtggcta tcgacacaga taaaaccggt catctgcggg
     6181 tcagggtcgt ggagggaaaa aaacatctgc gtgccgtcca ggaatatctg acccgcctgc
     6241 gcgcacgtca tcaggggcgt gtgggagact ttgagttcac caccctggat gtgatagccc
     6301 gcctcagaca ggacaccaca accgccggtg atgaaagcgt cattaacccg gtacagcaga
     6361 aaatgctcgg ctatatccgc cattctgcct ctcttggtgc ctcagacctt catatgaccc
     6421 cgggacgaga caacacggac ttcacctatg tggaagcccg ggttcacggc gaactggaag
     6481 tgctcgatat cctccggaaa gaagaaggcc tcgaattact gggggccacc tattccggca
     6541 tgaccgatgt tatcaaaggc acacagtttg atcccggggt gcctcaggat gcacgtcttt
     6601 cagagcagta tctgaaactg gccggtctgt ttggtgcacg ctactcccac tacccctgtg
     6661 tgggcggcct ctatgctgtg ctccgtctga tcaaggatga cagtcagcaa atcccgacct
     6721 tcagtatgct gggctatcac cctgaccagg agcgcactct tcgccgcatt ctgcagcgcc
     6781 ctgaaggcat tatcacgctc tcaggcccca ccggctcagg aaaatccacc acgctgagaa
     6841 ccgcctctgc ggcgtacctt gaacagtacg gcttcaacaa caccggcggt attcttcttc
     6901 cccgtcgcag gctgttcacc atcgaatccc cgccggaagg gcgtattccc ggtgccattc
     6961 agaccgccgt catggatacc gcgcagggct gggtggactc catcaaatcg gcactgcgtc
     7021 ttgaccccga cgggatcctg aacggtgaaa tccgcgacca cgattccgcc attacggcaa
     7081 taaaagccgc catgaccggc catctgatgc tgaccaccat ccatgcgaac gacccgatta
     7141 acatcctcga acgtcttgag atggaggggg tacaggccag gatgattgcc gacccgcaac
     7201 tgttcattgg cctgctgagc cagcgtctgg ttcagctgat atgtccgcac tgccgtcgcc
     7261 cctggcatga ggtggcgacc gagcgcacgg atgatgaacg gcgcctggtg gaaagcgtct
     7321 gcaacccgga ccaggtatat ctgcgaaatc atgaaggctg cccgcactgc tggcgtggcg
     7381 tgacaggacg aacggtgatc gcagaagtca tctccccgga tgcccgtttt ttccagttgt
     7441 accgggaaaa ggggcgtatt gaggccagaa catactggca ccgggagctg ggcggcatga
     7501 cacgaaatca gcatctgctg ggaaaaatca acagcggaca ggttgatccg ctggcggccc
     7561 attacatctc tcccatcgac gaggacagct atacgctgct gcactgacat gagatttctg
     7621 gaaaaactca acaacaccga cattgagctg acagcactga ctgtctggat agccagaaaa
     7681 acctttgccc taaaccgggt ggagttctac gaagccctgg gcggcatgat acgtgacgga
     7741 acgccgtccc tgcgtgcact cgaatttctc tgcgagatcg aaaccgattt tggtgaaaag
     7801 aaaggcacat ccggcatgta ttttctggca aaagaatgca tcgattccat caaagccagc
     7861 ggcatgttat ccggtgccct gttaacctgg gtgccaccgg aggaagccac gttaatcaga
     7921 aacggggaag agcgcggaaa tattgccgac gcccttttcc aggtggccag aaccgttaaa
     7981 gcccggcggg aaatgacatc cagcctcatt gccgtctgcc tctaccccct tctgctgctt
     8041 tcgctctgtg tggtgaatat gtacaacgcc catacccgta tcatccccat cgtcagtgcg
     8101 tttgcttcag aagataaatg gtcattccag atgaaattgc ttgccggcat gtcggaattt
     8161 ttcatcgggt acggattctg gctctgtggg accattttcg ccctgacgct tctgatccgt
     8221 ttttcattca gccggtatac cggccccggc cggaggatgc tggacaaggc tcccccctgg
     8281 tcactgtatc agaccttaca cggggtgaat tatctgttca gtatttcatc catgctgcag
     8341 attaatattc ctctttcgca ggcgctgctc cgaatggaaa aaggtgcaga aaagaataaa
     8401 tggttaaagg agagaattca ggcaatacga aaacatgtat tatccggtca gaatctggcc
     8461 acagcaatgc gaaattccgg ttatgatttt ccgtccagaa tatgcatcaa taaactgctc
     8521 ctgaactccg aaagggagga cagtgtggca accatggcac tctatgccga tcagtggctg
     8581 gaggaagcaa aaaaacgggt aaaacgaatt ggattaatta ttaccggtat atcaggaggt
     8641 tttgtcttct tttttattct taacatgata tctgccatct acagtatcag cgacatgctg
     8701 aaacaataat cccgcataca gaatatttct gacccccata acactaggag tgatatatga
     8761 acttcagatc atgtctttat tcttattttg ctttaaaaaa agagaaagag gaggataaag
     8821 gtttaacatt actggaaatt gccggtgcac tgattatcat cggtattgtg gtagctctcg
     8881 caatgagtca gatgtccagc acaatgtcat ccagtgataa ccagcgtgaa ctgaaaaacc
     8941 tgcagactat tgcaacaaaa atgaagcagt acaagttcca gggcagatat accggcacag
     9001 attatatcaa aaccctgaca gaaagcggtt ccctgccggc tgatatgatc gccggaagta
     9061 acaaggccaa aaatgcatgg ggaggcgatg tcaccattgc agcaacaagt aataaatacg
     9121 gttataccat aaccagtaaa gccgtaccga aagaaaactg tgttgagctg attaacagtc
     9181 tgcgcagttc atccatgttc acaaaaatca aaggccagac acctgccagt gtggatccgg
     9241 taactgtatg ctctgccgcc acaaacgata tcacgctcga aaccagcagt taatatcctg
     9301 acacagcgcc ctccggggcg cttttccgga gtgtatccgc atgaagtggt tatttttttc
     9361 tgccgtgatt tgtccgttat ttttcgccct tcccgctgag gctgtcgcca caaacacatt
     9421 actggacagt tgctttattc aggccggaaa acgctaccag attgcgcctg atcttcttaa
     9481 aaccattgca cagcaggaaa gcagtctggt cgccactgcg ataaatcaca ataaaaacaa
     9541 atccggaaaa atcatcagta cggattacgg gattatgcag attaattcag tgcatattcc
     9601 tgagctgaaa aagcttggag tcattaaaga caaaaatgac ctgcttaata atccctgcct
     9661 gaatattcag accggggcct ggatactggc ccgccatttt caacgctgcg gcgttaactg
     9721 ggaatgtctg ggcagctata acgccggttt cagcaaaaat aacacagccc ggcgcatgat
     9781 atatgcccga aaaatccaca accggtattt cagacaatat taataaatac gaggtgacgg
     9841 tgagcgcatt actgagcatg acaataataa tcatcatcct ggcggttatt ctttatcttg
     9901 catacagcac cgccgctctc tacctgtcag cagtacacga cacccggccg cccaggccgg
     9961 tggtgtacgt gtgttgcctt ctggccatag tgagtgtcag cctcaacggc gcatatggtg
    10021 ttgaagccac ggggctgtta ttcctttcca ttctgacggg attattaact gtaatgacgc
    10081 tgacggacat cgccgtctgt cggcttcccc ggatattcac gctttcactg attgttctgg
    10141 gggcagcgtt caggtacagc ctggaggagc tcacatacag cctcctcaac gcatcactct
    10201 ggttcggcat gacatacctg ctacggcagt ttttcataac ggcaaaagga acagaagcac
    10261 tcggccttgg tgatgtattc ctgatagcag gaattgcaat gtggacacag ccacaacaca
    10321 cgccgctgct aatcaccgcc gcagcttccg gggcatttct gtttatcctc ctcttctgcc
    10381 gtcgcagacg tcagcaggca ctgccgttcg ccccgtttct ctgcgcatca ctgtatgcac
    10441 taaccctcct ccctgattcc gttttccgca cttcggagat attcacgtga aaaagttcag
    10501 acacgatgac gacaaaggtt ttattctgct tgaaatactg gcgggactga tagtgattgg
    10561 tatcgccacg ccgctgattt attccgaaat agaaaactgg ctgaatgaac agctttacca
    10621 gagtgctgct tctcatgcag atgcctacaa taccgcagcc agaaattaca ttgccgataa
    10681 caatgccact ctgcacaacg gcagccttcc ggcaaacttt acagccgatg atttgattcg
    10741 caagggatac ctgaaacagg gatttaatcg ttctccgttt gggcagagtt atatcacggg
    10801 tatccgccgt aaccagacta caggacgtct tgaagccctg acctgctcaa caggggggca
    10861 gaatatcaaa gaagatggtc tgcgcagtgt ggcaggacag cttcccgggc tgggtggtta
    10921 tattggtaaa aacggaacag caaccggcgc attcggcgcc tggacagaca aaccggggga
    10981 ttacggactc acctgttcgg caggtcacat tgccgtcgtc atgacgggtg atgatctgca
    11041 ggaaagcgac aggttgtatc gttttcaggt cccggggaga ccagagctga accagatgca
    11101 caccgccatc aacatgggcg gaaatgacat caataacgcc ggtaacctca atggtcagaa
    11161 agccacggtg aaaggcgata taaccagcga agacggctgg cttatcacca gaaacaacaa
    11221 ggggtggatg aacacgacgc acggaggcgg tttcaccatg actgacagcc agtggatacg
    11281 cgccgtcaat aacaaaggga tcaccaccga tggtgaaatc aaagggggca aagtcagcgg
    11341 tggaacgatc cggtctgacg gtcgtctttc cacaggtgag tatttacagc tggaaaaaac
    11401 agccaccgca ggtacttcct gctcaccgga cggacttgta ggccgcacca gtacaggggc
    11461 catactttcg tgccaatccg gtgtctggaa gaataatatc agcgatgcac tggtttttga
    11521 ttattcggcc tgttatgacg cagaagaaga ctccagaaaa ccggtttatg cgaactgggg
    11581 tagtggctca aacggctttg ccgtctgtga cggcaaagca ggctatggtg cggcaggatt
    11641 cagagcgtac agaggtaaac cggggtattc gggaagcatg gtctgggtca aaatacgttg
    11701 ctgtaagtta aaagttaata cgccttaaat gcatcagctt gctttgacga tgtagttaaa
    11761 ggcaatgttt ttggggcgca tttccgttgt gcttccctgg gcccagtaag gcataccgct
    11821 gtgatgccct ttcagccatc ccccacgctc atgatagaca ccggattgat cggcctgata
    11881 actcaggaga tgtctgttcc gatctccatc cagcccacgt ccgttgtccc agccacggat
    11941 aaaaacaccg cgcaaatccg gcagcttaca cccgggataa gcacgggcaa gctgaggata
    12001 ccgggaacat gaaaatgact gcccgttcat caccaggtag ccttgtggag gggtggctga
    12061 tggccacggt atagggcttc cgacagggta ggattcaaaa cctgcccaca taccggattg
    12121 gcacgaaagt attgcatttc tgtatccagc ctcctacctt ccccgttttt atcgtggaag
    12181 gcagatatgt ttcaaaaaaa caaaactcgc aaaatgtccc tcagccgggc actggacaaa
    12241 tacctgaaga ctgtttccgt ccataaaaaa ggtcatctgc aggagtttta tcgcgtcaat
    12301 gtgattaaac gtcaccctat ggccgatcgc tatatggatg aaatcactac cattgatatc
    12361 gccggttacc gggatcagcg tttagctcaa attaacccac gaacagggcg tcaaatcacc
    12421 ggaaataccg tccgtcttga acttgccctt ttatcatcat tattcaatat cgcccgtgtg
    12481 gaatggggaa catgccgtat gaatcctgtt gaactggtcc gaaaaccgaa aatcagtaat
    12541 ggccgcgacc gccgactgac ttctggtgaa gaacgccgca tttcccgcta tttcagggac
    12601 aaaaatccgc agctctacgt tatcttccat ctggctctgg aaacagcaat gcgccaggga
    12661 gaaattctct cattaggctg ggaacacctc gatttgcagc acggcgtggc gcatcttcct
    12721 gaaacgaaaa atggctcacc acgcgacgta ccactctccc ggaaagcacg taactacctg
    12781 caaattctgc cacaacagat aaacggcaat gtgttcagtt acacctcgtc cggtttcaaa
    12841 agcgcctggc gtaccgctct tctggatctg aaaatagaaa atctgcattt tcacgatctc
    12901 cgccatgagg ccatcagccg tctgttcgag ctcggcacac tgaacgttat ggagattgca
    12961 gccatttccg gtcaccgctc cctgaacatg ttgaaacggt acacacatct ccgggcatat
    13021 cacctggtca gtaaactcga tgcaaggcgt aaacagacca gcaaaatttc cccttatttt
    13081 gttccctacc ctgcaactgt cagaaacaga aacggagtgt tcattgtgac actgcacgat
    13141 ttcgaccttg aaagccgtgc tgaaacccgg gaactggcaa tatctcacgc cggcgtattg
    13201 ttgctgcgcg cactggccca ggctgctcag cggggtgaac gcgtccccac accaggcgag
    13261 ctacccgcaa acattgatga acgggtcatg atttgtccgt taacaaacag ccagttcatg
    13321 acaaaagcga acagtatttt catatcctcc tcctgaacaa ataatttttg ttcaaaatta
    13381 acgctatgag atcctttatg aaacacttat cctcccgtgc agccactgcg gataataatc
    13441 gtatcgttac aattgaagac gtttacacaa tcgcattctc gcaaccaatt cacaccgaat
    13501 cagcgttggg gcaaaggtgg atgttgatcc atactggtga atatgaaagt gccagcgatg
    13561 caaaacgctt ccttgatacg ctcgtcaccg ggcactgtgc ttgcatggta acaccatcac
    13621 ctgttctgga tcatgacacc tggctgcgta tccgcctgct gatgggcgag ataagggatg
    13681 ccaaataaac attcatggct ccggaacata aaaatagagc cgggaacgcc gggcactgcg
    13741 tgctgctcag cggagtaaac acgttcccac atcaggcgag ctgcccgcaa acattgatga
    13801 acgggtcatg atttgtccgt taacacactg aaggcatatc agaatgaaaa taaactattg
    13861 cttccgggtt aaattaatca cggtcatcct gtctcatttc ctgatacaga tatatatgtg
    13921 gccagatttt ctgacagcga aaaatattcc ccgtctcatt gccgctatgg tagctctgta
    13981 cctgtctgca gtaactgtca ttccggcatt tttactgtca gatgacagtg agagatttaa
    14041 aaacctctca cctttaaagc aaacaatatt attagttctg tgtacagttg gagtaacatt
    14101 tttactctta catggattta atacataaac accttatgaa aaatccctat tatgttataa
    14161 ataataaaag actcagagga taacacccat attgcgacaa gcactcgcat tatatttaca
    14221 ctttctgtaa gttcacttat ataaataaaa acaataaaaa accaccacag agaaacgatc
    14281 attaaagaga tacagtaaac aaatatacac aacacactca tcaccgacca gggtttctcc
    14341 agcatgacaa aaatcattga taccagcacc acaatagctg cattacctaa cagagtttca
    14401 cgaataatat tccttataat ttccagtttt tccttatgct gttttatttt catactaacc
    14461 tcattgattt tatcccgaaa aggaaacgat gatacaccag aatactttca cggcgactct
    14521 gcagcataat gccagaccag cggcatgctc agtcacttcc tgtccgaatc cttttcattc
    14581 gcccttaaaa cagagttgca aacactcttg aaacacactc gatcataacg atcgaaataa
    14641 ctcgtttccg tttaaaaaac aaactctcta gactcaattt atgaacaaaa cgtgctgccc
    14701 cgcatggagg tgaaatgaaa cctgacagta caggtattgc cacccgcatg atgctctccc
    14761 tggacagaga gcgaatttgc gaatgcctgc tcagtaatcg ccagttgcag tccactcccc
    14821 ttcaggtgcg ttatccgcag ggagtcaggg atgccctggg tatcatgagc gaacagcttt
    14881 ctctttccgt ctctgacctg acgcgtatcc tggtcgaaga ggcgttaagt gaaatgtttc
    14941 tccctgcaga taacatcgtc cgccgtcttc tgtgcagaat ggaacacatc atgcaggcac
    15001 atgatatcag cgcaacaacg atggctgcat tgctgacccc ctggaatatc cgccctgccg
    15061 tcttccggga gccggacaga ctcactgact atctgaccgg ggatattctt gccgcactgg
    15121 ctgactggtt ttatctcagc ccggaatggc tgaacggcag ggtgcattat ccgctgtatc
    15181 gtccggggga ctggcctgtc acacaggagg cattctgcag gattatttct tccggtgaga
    15241 atgtggacat tattctctgg catggttttc cgtttgccgg aacgcattcc ggggaatatt
    15301 gcggtgttct gctcaggcag aaaaaagaga ttaataatgc cattatttat ccggtacttt
    15361 cgttatatcc ggtcagaatg gatatggaaa aagaaggctg gtttcagatg gtacgaaaaa
    15421 tatctccgga tatccctgta cgcgccgtaa cgctcactcc ggcacaggca gaatatctga
    15481 taacagggaa aatattaccc actgtgttat tcagggtctc attatcaccc tggtaataaa
    15541 actgagtaat aacccgaaat aaataacaca ccaacttttt aatggcacaa ccgtgccctc
    15601 tgttgttata cccaaaataa ctcaatacag gaaactgaaa tgatgatgaa aaagcatatt
    15661 acccgtactc tcatcgcgtc tgccgtttta ttttccttta attcaacagc cgcaacttca
    15721 tattttgaag cccgcaatga tgcaatgggg ggaaccgggg tagcctcctc tcactacggt
    15781 gtggcacctc tggcaaaccc ggccctgctg acaaaagccg gtccggaaga tgacttcagt
    15841 cttctgcttc cgtccgttgg tgggcaattg tccgatccgg gtaatatcag tgacaacgca
    15901 gacaaaatca gtgatgactg gaaagccttt gatcgtgcca tcgacagtca gagcaacgtg
    15961 tcgcagactg ctgcacgtct gaagacgcga cttcaggact tccgtcacac gcatgctgac
    16021 gcacaggtgg gaacatctgc tgtggcgact cttcccggtg acaccctcgc tgccgctctg
    16081 atgctgaaaa gctacggtac ggtcagtgtc gacggtaaag tcagtgatgc cgacctgaac
    16141 tatctggaaa gcattgcaaa cagtaacggt caggatgtgg ataaagacag actgacctct
    16201 caggcttttg cccgtgccgc actggtcacc gatgtgggta ttgctctggc tactgaactg
    16261 gaaacggccg gacaaaagtg gtctctgggc ttcacaccaa aattccagcg tgttgacctg
    16321 tttaactaca acgtgctggt taaaaattac gacagcagcg ctttcaaagg cgaccgttac
    16381 cacaacacca gaaacggcat taacgctgat atcggggcca gcatggacct ggatgacaac
    16441 tggacactgg gtctggtcgc acagaacctg ctcccccgca gtattgagac aaaggaagtg
    16501 aacggtgtca ctgaaacttt caggatccgc ccgcaggtga ctgccggtgt ctcctggcac
    16561 aacgacatgt tcactacagc actggatgtt gacctgaccc cagccagtgg cttcacatca
    16621 gacagtaaac gtcagtttgc tgccattggt gctgaattta atgcctggaa atgggcacag
    16681 ttacgtgcag gctaccgcca gaatctggcc agcaatgatg gctgtgcgtt caccgccggg
    16741 ctgggtatct caccatttga tgtcgtccat attgatgtgg cgggtatagc aggcacagac
    16801 aacaactacg gtgccgttgc acaactgcag ttcacattct gatcatctct gccccggtac
    16861 tgaccggggt atccagacct gcatcctcgc gttttccgac catcaaacga cacagccaga
    16921 cctgtagtgc tgcctctgat ctggctgtta tctgacatat cattttcgca tgaggaaatg
    16981 catgtttaac aaaaataaaa cgcatatcga aaacaaagcc tttatggtgg aaccgttccg
    17041 ggatccgaca gctaacgaat gtaatgtcgt gatagcaaac gatatttgta tccgggtagc
    17101 actgtcaccc tctgaggtgc tccagccatt cagcaaaaac acgaaccagt aaatcctccc
    17161 cacaacgctt tcttctttca ctgctcagga aaaatacacc atgaaccgta tgatacctgc
    17221 gggatgtctc gccgtactct gcctgaacgg ctgccatctc cccccggata atacagctcc
    17281 gaccagaaag tatggtgctg cctctgatgt cgccgtcgcc cggcatgcgc agtatcagct
    17341 atgggggcag ggaatttact ctgcagccct tccggagcgt cagtccggcg gcagtctgaa
    17401 ggctcacagc gaccgtgttt cctttgactg ggaaggcgat gccctggaat tgctcaacga
    17461 actggcccgt ctgcgcggcc agcagttcag ctacagcggc gtccgtctcc ccttacccgt
    17521 ctcactgcac gtccgcgaca tgacctttgc tgatgtcctt cgtctcactg aagcccagac
    17581 tgcctggcgg gcaacactct cgcagtcagc cgggcgttta catctgtctt ttatgacgcc
    17641 ggagaaaaaa tgaaaacaca cctgtgtctc ctgctgtctg ctctcctgct tacaggctgt
    17701 gccggacagc atcaccgttc ttctgccgtc atgacagaaa acgcaccgcc cccggatatc
    17761 cgtgcatggc tgacgccctc cggcaaccag gatgatggta tttcagatac ccgtcgtcag
    17821 atgctcacgg atgccggcag aacgctggga ttccgtggtg gaaaagcaca gcgctcatgg
    17881 gaactgattc aggcactgaa tgcccgggaa agtaccctga atgccctgta tgatttccgg
    17941 cctctcatca gcaaggaagg ctggctgcct cctgttatcg atgaagctca ggatgttgca
    18001 cacatcagac cggaccagat aagaacagcc tcccgtgtct ggactatcct ccgcccggaa
    18061 cgctttgtca gtaatccacc tggatggcgt gactggctgc tgaaaggact ctcaaccacc
    18121 gccacaccag gcactgaagg ccgggttgtg ccggaagacc gggcacaacg cagactatgg
    18181 gaaaacgcct tacgtcaggg atggcaggaa gggcgcctca acgcagatct cacactggaa
    18241 gctaaccaga agcgcctgac ccgtgattac cggggcatga tgctttacgc ccttctgtgg
    18301 cgtcagggca tgatcacacg tccagatgtc acagagcaga tgcagacggt taccggcaac
    18361 gggcaaaaac tcatcactgg cgatcgcgtc cgccgcctga aaacacatgc tgaatttacg
    18421 ctgcagaaat cgcgctggcg tcctcttgtc agcaccgaag gagagaaaaa tgatatcacc
    18481 agatgacttc ggtaccttcc ccttctcccg ttttacggca gaagagtttc gccatttttt
    18541 tgcctggtgt gcccgccacc gcgtcagtga tattgatctg accggcggtt caccggtatc
    18601 cgtcagtcgc tttggacggc gggttcgctc atccgtaaat ccccttccgt cctccatcat
    18661 gatggcactg agtgatgagc tgtttgggcg ggaggttctt ccccgggttc tgtcaggtca
    18721 tcccgtggat cgtaccgttc aggtcagcgg agatgcctcc ggtcgctatg gcctgcaacg
    18781 aggtcagcgt gttcgcctgc gctgccacct gatacaggga acatcggcaa cagaagaaaa
    18841 agccatttcc gtcaccatgc gggtgatccc gtcagatatt ccggacatcc tcactatgaa
    18901 tattgaaccg gatctgctgg atgccatgct cagaaaaacc ggcctgggct tcatctgcgg
    18961 agaaaccggc tccgggaaat ccacactggc ggcggcactg tatcgccaca ttctgaaaaa
    19021 tttccccgat cgcaaaatag tgacctatga agatccggtg gaatacattc tgggcggaga
    19081 gaatgacctt ctgcctcccc atcaggccga aatcgggcgc gatgtggaaa gttttgctgc
    19141 aggacttcgc tctgccgttc gccgtaatcc ggacatcatt ggtatcggtg aaatcaggga
    19201 ttatgaaaca gcagatgccg cagtccaggc gggtaatacc ggacactttt gtatcggcac
    19261 catgcacaca aaatcacccg gagaaacctt tgcccgcctg ctgggattgt ttcctcctga
    19321 aatcagggat tcgatggctg cagccacgct ttccctcgta cagttcattc tg
//
LOCUS       DAZGSP010000094        19340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZGSP010000094 DAZGSP010000000
VERSION     DAZGSP010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.094"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            151..432
                     /locus_tag="ACHK74_003758"
     CDS             151..432
                     /locus_tag="ACHK74_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222502.1"
                     /translation="MTEAEILGLIRRVAGISQQADEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHWKKPERLRR"
     gene            1588..1908
                     /gene="clpS"
                     /locus_tag="ACHK74_003759"
     CDS             1588..1908
                     /gene="clpS"
                     /locus_tag="ACHK74_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFP0222503.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            1939..4215
                     /gene="clpA"
                     /locus_tag="ACHK74_003760"
     CDS             1939..4215
                     /gene="clpA"
                     /locus_tag="ACHK74_003760"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFP0222504.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(4559..4646)
                     /locus_tag="ACHK74_003761"
     tRNA            complement(4559..4646)
                     /locus_tag="ACHK74_003761"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4610..4612),aa:Ser,seq:gga)
     gene            complement(4900..5118)
                     /gene="infA"
                     /locus_tag="ACHK74_003762"
     CDS             complement(4900..5118)
                     /gene="infA"
                     /locus_tag="ACHK74_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFP0222505.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(5403..6107)
                     /gene="aat"
                     /locus_tag="ACHK74_003763"
     CDS             complement(5403..6107)
                     /gene="aat"
                     /locus_tag="ACHK74_003763"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFP0222506.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(6149..7870)
                     /gene="cydC"
                     /locus_tag="ACHK74_003764"
     CDS             complement(6149..7870)
                     /gene="cydC"
                     /locus_tag="ACHK74_003764"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFP0222507.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(7871..9637)
                     /gene="cydD"
                     /locus_tag="ACHK74_003765"
     CDS             complement(7871..9637)
                     /gene="cydD"
                     /locus_tag="ACHK74_003765"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFP0222508.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(9760..10725)
                     /gene="trxB"
                     /locus_tag="ACHK74_003766"
     CDS             complement(9760..10725)
                     /gene="trxB"
                     /locus_tag="ACHK74_003766"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFP0222509.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            11270..11764
                     /gene="lrp"
                     /locus_tag="ACHK74_003767"
     CDS             11270..11764
                     /gene="lrp"
                     /locus_tag="ACHK74_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFP0222510.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            11899..15810
                     /gene="ftsK"
                     /locus_tag="ACHK74_003768"
     CDS             11899..15810
                     /gene="ftsK"
                     /locus_tag="ACHK74_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP0222511.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPRQQDTLLHPLL
                     MRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVV
                     NYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPN
                     KKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKS
                     VGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWC
                     VNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEP
                     YIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANI
                     PTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAV
                     VQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQ
                     RQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            15965..16576
                     /gene="lolA"
                     /locus_tag="ACHK74_003769"
     CDS             15965..16576
                     /gene="lolA"
                     /locus_tag="ACHK74_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFP0222512.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FTAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            16587..17930
                     /gene="rarA"
                     /locus_tag="ACHK74_003770"
                     /gene_synonym="mgsA"
     CDS             16587..17930
                     /gene="rarA"
                     /locus_tag="ACHK74_003770"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFP0222513.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDRGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            18021..19313
                     /gene="serS"
                     /locus_tag="ACHK74_003771"
     CDS             18021..19313
                     /gene="serS"
                     /locus_tag="ACHK74_003771"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFP0222514.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
BASE COUNT         4930 a         4765 c         5167 g         4478 t
ORIGIN      
        1 tcatcatcat gtgtgtaacc tgttgttttt aatgtggttg atgcaaaaaa gatgatggtt
       61 gtggttaatt tttgttcgac atcttttagc gtgacagatt ctttacaaaa aatctgagct
      121 tgttttcttc accagcgcga tgggggcaca atgacagaag ccgaaatact gggattaatc
      181 cgccgcgtcg ccggaatcag ccagcaggct gacgaacagg ccacgcagcc ggacagcgtg
      241 accgccgaaa attatgcgcg tgtggttgct gaggtgatgc gccgtgatgg tattgagctt
      301 aacggcgtgg atatgcgcaa catacgaacc agagtccttg agttgctggc ataccgtcgc
      361 cgcgtggaga tgtatcggga gaaggagaaa atcacgtacc actggaagaa gccggagcgg
      421 ttacggcggt aactcactga gattaaagca aagctcaaaa ttgcgctggc tgaatgatca
      481 ttatgattac ttatgtttac gcagatgatt aaggaatgac cgaaggcgga aattcgcctg
      541 tggttgatgg gggagttgca gatctgcaac tcgaccatga aattacggaa actacccgta
      601 gtttgggtag taagagtaac acccagattt tggggcttac tcgcgatacc caaataaagg
      661 gtatcggtgg aagaaatatc gtttctcata tgtgagtacc gagggcggaa ttccgccttc
      721 ggttactcgc tgaacttaaa gcaaagcgca aaattgcgct gattactggt tggaattgtt
      781 ccagagtgca aatttgcact ctggcctaac ccgttgatat ttccggcaac ctcaatttga
      841 ggagatcgga aacatcaccc ttcaaattct gaatggtcat aatgaccacg cagaccatca
      901 aggcaaccac aacgaacatc gttacggttg gatcttttac cccactgggg aaaaggtacg
      961 cacctgaatc atcattaccg tgatcaaggg tatgtaaagc ccaccagcct gattaacagt
     1021 taaccggaaa aaaaatccgg tatggggggg agtggttagc cacgaacaac gaacaagctg
     1081 gacaccgcgc cccctctcac gcagagaaaa aattaccgtt tcgtagtagt taacatgtta
     1141 actgcctgat gaagcgttgt tgttgatttt ttctttatga ttcatctggt tgcgagcaaa
     1201 aaatgttaac tacctttttg gtaaaaatgt taacagggca gcggttaaca tttaacctgg
     1261 caaaaattcc ggtaggtggg gatccccata tcggggaaca tccaccagct tcataacgga
     1321 tagccggaaa atgataacgg ctaaagcgtg ttgatctttg ttcgtggttg ttcgatattg
     1381 ttcgtgaaac ggtgtagtta atggtgtagt taatttcgct gtttttgaca gagttgttat
     1441 ttgtaacgca atgaaaaata aagactaatt aacgtaatgt tataaatgat agttatctat
     1501 catgtggagt agattggtca ggcaaataag ctcttgtcag cggcagggcg ttctgccgat
     1561 aaccgtaacc gaagatgata actgacaatg ggtaaaacga acgactggct ggactttgat
     1621 caactggcgg aagaaaaagt tcgcgacgcg ctaaaaccgc catctatgta taaagtgata
     1681 ttagtcaatg atgattacac tccgatggag tttgttattg acgtgttaca aaaattcttt
     1741 tcttatgatg tagaacgtgc aacgcaattg atgctcgctg ttcactacca ggggaaggcc
     1801 atttgcggag tctttaccgc cgaggttgca gaaaccaaag tggcgatggt gaacaagtac
     1861 gcgagggaga atgagcatcc attgctgtgt acgctagaaa aagcctgaat gcaggcataa
     1921 aaattggggg aggtgcctat gctcaatcaa gaactggaac tcagtttaaa tatggctttc
     1981 gccagagcgc gcgagcaccg tcatgagttt atgaccgtcg agcacttgtt actggcgctg
     2041 ctcagtaacc catctgcccg ggaggcgctg gaagcgtgtt ctgtggattt ggttgcgctc
     2101 cgtcaggaac tggaagcctt tattgaacaa accacacccg ttctgcctgc cagtgaagag
     2161 gagcgcgaca cacagccgac gctgagtttt cagcgtgtac tgcaacgtgc ggtcttccat
     2221 gtccaatcct ccggtcgcaa tgaggtaacc ggtgcaaacg ttctggtcgc tatctttagc
     2281 gaacaggagt cgcaggcggc atatctgttg cgtaaacatg aagtcagccg tctcgatgtg
     2341 gtgaacttta tctctcatgg cacgcgtaaa gacgagccga cacagtcttc tgatcctggc
     2401 agccagccaa acagcgaaga acaagctggt ggggaggaac gtatggagaa tttcacgacg
     2461 aacctgaatc agcttgcgcg cgtgggcgga atcgacccac tgattggtcg tgagaaggag
     2521 ctggagcgtg ctattcaggt tctctgccgt cgccgtaaaa acaacccgct gctggtgggg
     2581 gaatctggtg tcggtaaaac cgcgattgcg gaaggtcttg cctggcgaat tgttcagggc
     2641 gatgtgccgg aagtgatggc tgactgtacg atttactctc tcgatatcgg ttctctgtta
     2701 gcgggcacta aatatcgcgg cgactttgaa aaacgtttta aagcgttgct caagcagctg
     2761 gagcaggaca ctaacagcat cctgtttatt gatgagatcc acaccattat cggtgcgggg
     2821 gcagcgtctg gtggccaggt cgatgcggct aacctgatca aaccgttgct ctccagcggt
     2881 aaaattcgcg taattggttc gacaacctat caggagttca gcaacatttt cgagaaagac
     2941 cgtgctctgg cgcgtcgctt ccagaaaatt gatattactg aaccgtcgat cgaagaaact
     3001 gttcaaatca tcaatggcct gaaaccgaag tatgaagcgc accacgacgt gcgttatacc
     3061 gcaaaagcgg tgcgtgcagc ggtagagctg gcggtgaaat acattaacga tcgtcatctg
     3121 ccggataaag ccattgacgt tatcgacgaa gcgggcgctc gcgcacgcct gatgccggta
     3181 agcaaacgca agaaaaccgt taatgtggcg gatattgagt ccgtggtggc ccgtattgcg
     3241 cgcattccag agaagagtgt ttctcagagt gaccgcgata ccctgaaaaa cctcggcgat
     3301 cgcctgaaaa tgctggtctt cggtcaggat aaagccattg aggcgctgac tgaagccatt
     3361 aagatggcgc gtgcaggttt aggtcacgaa cataaaccgg ttggttcgtt cctgtttgcc
     3421 ggccctaccg gggtcgggaa aacagaggtg acggtacagc tttcgaaagc gttgggcatt
     3481 gagctgctgc gctttgatat gtccgagtat atggaacgcc ataccgtcag ccgtctgatt
     3541 ggtgcgcctc cgggatacgt tggttttgat cagggaggtt tgctgactga tgcggtcatc
     3601 aagcatccac atgcggtgct gttgctggac gaaatcgaga aagcgcatcc ggacgtgttc
     3661 aatattctgt tgcaggtgat ggacaacggt acgctgaccg ataacaacgg acgcaaagcg
     3721 gacttccgta acgtggtgct ggtgatgacc accaacgccg gggtacgtga aactgagcgt
     3781 aaatccattg gtcttatcca ccaggataac agcaccgatg cgatggagga gatcaagaag
     3841 atctttacac cggaattccg taaccgtctc gacaacatta tctggtttga tcatctgtca
     3901 accgacgtga tccatcaggt ggtggataaa ttcatcgtcg agttgcaggt tcagctggat
     3961 cagaaaggtg tttctctgga agtgagccag gaagcgcgta actggctggc cgagaaaggt
     4021 tacgaccggg caatgggcgc acgtccgatg gcgcgtgtca tccaggacaa cctgaaaaaa
     4081 ccgctcgcca acgaactgct gtttggttcg ctggtggacg gcggtcaggt caccgtcgcg
     4141 ctggataaag agaaaaatga gctgacttac ggattccaga gtgcacaaaa gcacaaggcg
     4201 gaagcagcgc attaatctga ttgttaggta ggttggtcaa gtccgtaatc tcgaaagagg
     4261 ttacggactt tttgtttatg gggtggagga ggttcagacc ctttttttaa tgatgatggt
     4321 aagttgttga taattagtgc tgcgggaagg taaggataaa aaagggtgct gcaggagaat
     4381 gggatggttt tgctttatta acaacgggct aaacgtgtag tatttgagtt cactgccgta
     4441 caggcagctt agaaatcgac ggggtgcggt aaaacctttg cgaacgcgtt cactgccgta
     4501 cagacagata aaatgcgaaa aaaaagctcg cactttcgta cgagctcttc tttaaatatg
     4561 gcggtgaggg ggggattcga acccccgata cgttgccgta tacacacttt ccaggcgtgc
     4621 tccttcagcc actcggacac ctcaccaaat tgttttgtcg cctgacctca tgggtggcaa
     4681 cggggcgcta ctatagggag ttggagtaaa acggtcaaga agaattttaa tgataattat
     4741 tgtttgctca tactgtaaac aagttgtgca gtatatctac atcgagacaa gttacggact
     4801 tatacttcca aagtacttca tacatatcac aaaataaaaa ggccggttaa accgaccttt
     4861 tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat cagcgactac ggaagacaat
     4921 gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc actttgtcgc ccgtcaggat
     4981 gcggatgtag tttttgcgca ttttaccgga gatgtgtgca gtaaccacgt gaccgttttc
     5041 taactctacg cggaacatgg tattaggcaa cgtttcaaga acggtacctt gcatttcaat
     5101 attgtcttct ttggccatct aatcctctgg ggtatcacta ccgtaatttg aaccggcaag
     5161 ataatgccga agttctgtaa ataagtaaag atttgcgcgc taaatcgcaa caaacaggtt
     5221 cggcacatta ctccgaaaac acacggctaa gccgcaccaa aagcgcaacg tataagggag
     5281 cggtgagata aacgatgggc gttacctgac gcgaaaaatt ccttatcggc agcggggtaa
     5341 tgagcgtaac caactctgcg accgcaatta taacactctg gggagaaatg tgccgaaaac
     5401 attcattctt gtggtgaaaa caagcatcgt ggtacccaga aattattcgg caatcgtccg
     5461 aggcgcattt gattgagata attaaggtaa tcccggcggg gaatttcgca ggcaccaagc
     5521 gatgctgtgt gatcgttaag gacctggcag tcgataagct taccgccatg accgataaat
     5581 tcctcacaga ataccagaag cgccgtttta gacgcatttt ccatccggct gaacatggac
     5641 tcgccacaaa atagcgttcc ctgggccacg ccgtacatac cgccgacaag ctcatcttca
     5701 cgccagactt caatggagtg ggcatggccg agttcgtgaa ggcgatggta ggcttcgacc
     5761 acgccacgcg tgatccaggt tccttcttcg cgatcgctgg cacagccttc aatgacctga
     5821 ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt tatgaaatcg cttcatacta
     5881 cggctgatat gcagtgattc tggccatagc accgcgcggg gatcgggcga ccaccagagg
     5941 atggggtcgc ctggagaaaa ccacggaaaa ataccacgct ggtaagccat taacaggcgc
     6001 gcagggctaa gatcgccccc aagtgccagc aggccgttag gctcacgtaa tgcgccttcc
     6061 ggggaaggga aggctattga atggcgagaa agctgaacca ggcgcatgac cgcaaaactc
     6121 cacgcaagtc ggatcgttca ataatagctt acaaaccctg cttgaactgg taataacgcc
     6181 cctgtctggc aagcagctct gcgtgagtac cttgctcaat aatttgcccg ttgtccatca
     6241 ctattatttg ttggaaacga gagagtccgc gaagtcgatg ggtgaccatt aacaccgttt
     6301 tctcacgcat catttctgca agcaattcaa ggatctggct ttcggttgtg gcatctaagc
     6361 cttcggtagg ttcatccagc aacaccagtg gcgcatcatg taacagcgca cgggcgatag
     6421 ccagacggcg cagttcacca ccggagagct ggcgtccgcc ttcacctaac caactgttga
     6481 gacctgcatc ctcgagcagc ttctccaggc caacgcgacg caagctctcc gccagagcct
     6541 catcactact gccaggcgag gcgagtaaca gattatcacg cagcgtggcg ctaaacagat
     6601 gcactcgctg aggaacaacg ctgatggtct gtcgtagagc cgcttcattc aggctggcta
     6661 tggggctatc gttaagcaaa atctcgccct gttgcgggtc ccaagcgcgg gtcagcagtt
     6721 gtaacagtgt tgatttgccg catccggttc gcccgagaat cgcaatatgt tccccggcgt
     6781 ttacctgaag agaaatccct ttaagtgcct gttgggattg ctccggataa gtgaactgaa
     6841 catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt ttgggtatca gggaaggtga
     6901 cttccggttt ttgatccgtt aaatcagtga tccgaacggc agaggcaatg acttgcccca
     6961 gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa cgcggctaac gcgcagaaga
     7021 caaacagggc aattaacgcg ccgggttgag cattgccgcc aacgccgcca gacgccatcc
     7081 acagcatcag gatcaccgct aacgcgccaa tgagtagcat tatcgcttgc gacaatgcgg
     7141 tcagttcaga ttgacggcgt tgcgcttcca gccattgaat ttctgtattc tctagttgcg
     7201 tgcgataacg atcgctggca ccaaaaatgg tcagctcagc ttgcccttgc agccaggccg
     7261 tcagttgttg gcgatactgt ccgcgaagat gagtcagatt ttgcccggtg ctttttcccg
     7321 cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa cataatgccg cccagcgtaa
     7381 aggcgagggt gaaatcaagg aaacttaacc cgattgtcac caccataatc accacaaaag
     7441 cgcccaccag cggcgagata acgcgcagat aaagatgatc gagcgtatca acatccgcca
     7501 ccacgcgatt gagcaattcg ccctgacgat agcgcgccag tccggcaggg gagaggggca
     7561 gcaatttgct gaaggtgtaa atgcgcagat gctgcaacac gcggaaagtc gcgtcgtgac
     7621 ttaccagacg ttcaaaataa cgcccggcag tacgggtgat tgctgcgcca cgcacgcccg
     7681 cagcgggtag catatagttg aagctgtaca gtccggcaac ccccgcaacc gctgaggccg
     7741 agaggaacca gccggaaagt gtcaacagac cgatactggc gagcagcgtg acaattgcca
     7801 gcacgatacc aagacttaac atccatttat gacgtttata cagtgccaga tagggtagca
     7861 aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa tgtggcgaat gggccaccag
     7921 ccacacttaa ttccgcgtaa cgtccttgct caataatccg gccatcctgc ataacccaaa
     7981 tgacatccca gtcagcaaga tcttctaact ggtgggtgac cattaacgtt gtctggcgca
     8041 gagaggcggc attcagcgcc tccattacgc gctgttcact gtgagcatcg aggctggcag
     8101 cgggttcatc caacagtaat agcgaacagg gatttagtaa cgcacgggcc accgccacgc
     8161 gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc aacaggcgta tcaacgcctt
     8221 gtggcaggag cggtagaaac tcgctgaccc aggcgttatc cagcgctgct tgtaattctt
     8281 gttcgctggc atcaggtcgc gccagtagta cgttatcccg caatgttgct gccggtaatt
     8341 gtgggttttg cccaacccag gagagatgtt tacgccatga ttctggtgat aaatcgcgta
     8401 attctatccc gttgattcgt aatgatccct gatatgaaag aaaaccagaa agcgcgttca
     8461 gcagtgagct tttacctgaa ccgctgcgac caaccaacac tgcacgttgg cctgctggca
     8521 aagtaaagtt cagcggtccg gccagcgttt taccttccgg cgacgtgata aacagatcct
     8581 cggcctcaat ggtcaccgga tcggtcgatg ctaattccgc ctcaccgcgc tgcggatggg
     8641 cgagcggggt ttccataaac gttttcagac tgtcagctgc accaacagcc tgggctttag
     8701 catgataaaa cgtaccgaga tcgcgcaatg gctggaaaaa ctctggcgca aggatcaggg
     8761 ccagaaaacc cgcagccagc gtcacaccgg tatcgtagtg accaaaatcc agctcgccga
     8821 gataggaaaa accaaagtag accgccacca gagcaattga cagcgaggta aaaaattcga
     8881 gaatgccgga ggataaaaac gccagccgta gcacttccat tgtccgttgg cggaaatctt
     8941 ccgaagcaga acgaatactt tcaatttcag cttcaccacg accaaaaata cgcaatgttt
     9001 ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg agcaagagcg agaaagttac
     9061 gccggttagc atcggcagcc cccatcccaa ccagcgccat aaacaacgga attaacggtg
     9121 ctgtgcccag cagaatgagc gccgcagccc agttagaggg gaagatcgcc accacaatca
     9181 gcaacggcac cgacactgcc agcgccattt gcggcaggta gcgtgcatag taatcatgca
     9241 tatcgtcaat ttgctcgagt accaacgtcg cccagctccc cgcaggttta ccctgaatcc
     9301 acgctggccc tgcttgttgc agacggtcga gaacctgacg gcggatggca aagcggatat
     9361 gctgcccggc gtgataaccc acccgttcgc gtaaccagac cacccatgcg cgcagtacaa
     9421 aggtcagaac cagtaacgta aagggaagca gcagggcttc acggggaata ttctccataa
     9481 tcatatgttg cagaattcgc gccatgaacc aggcctgggc aatgatcaat atgccgctta
     9541 caaagcccag cagacgagaa atattcagcc aacgttggga gatgacgctt tgctgtttta
     9601 accagcgggt taactctttt tgacgggatt tattcattgc acgcttagca ggtgagttat
     9661 cagaattatt tgcagagcaa tgttacaacg gggaaaaaat aaaggcgacc catagtcgca
     9721 tggtgtcgcc ttctttactt ttgttactga tttgtaaaat tattttgcgt cagctaaacc
     9781 atcgaggtag cgttccgcat caagtgctgc catgcagcct gtaccggccg aagtaatggc
     9841 ctgacgataa atatgatcca tcacgtcacc cgcggcaaag acgccaggga tgctggtctg
     9901 ggtggcatta ccatgaatac ccgactgcac tttgatgtag ccgttttcca gttccagctg
     9961 cccttcgaaa atggcagtat tcgggctgtg accgatagca acaaacagac cagcaacgtc
    10021 gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga cgaacgccag tgacacccat
    10081 ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc agaatgatgt tgccgttctc
    10141 cactttatcc atcagacgct taataaggat tttttccgcg cggaaaccgt cacggcggtg
    10201 aatcagatgc acttccgaag cgatgttaga cagatacagc gcctcttcaa ccgcggtatt
    10261 gccgccgccg atgaccgcaa ctttctggtt gcgatagaag aaaccgtcgc aggttgcaca
    10321 agcagaaacc ccacggcctt taaaggcttc ttcagagggc aggccgagat agcgtgcaga
    10381 agctccggtg gcaataatca gcgcgtcgca agtgtattcg ccgttatcgc cattcagacg
    10441 gaacggacgg ttttgcagat ccaccttgtt gatatgatca aaaatgatct cagtttcaaa
    10501 cttggtggca tgttcgtgca tgcgctccat taataacgga ccggtcagat cgtttggatc
    10561 gccaggccag ttttccactt ccgtggtggt ggtcagttgg ccgccttttt ccatgccggt
    10621 aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca gcagcggtgt atcccgccgg
    10681 gcctgaaccc aggataagca gtttactgtg tttggtcgtg cccatgagat ccccatagtt
    10741 gttggcagac aatgggcagg attgtaggga atttacagac gtaaaaaaag agtatgacga
    10801 ttttgttaac aatttgtgca atcggcagca tcgataagca ggtcaaattc tcccgtcatt
    10861 atcacctctg ctacttaaat ttcccgcttt ataagccgat taaatgatga ataaacgccc
    10921 ctgttaatga atatctggca tgttgtacta aaaatcgatg ttttgctttg acaatcccct
    10981 ggtgttttgc gaaaacattc gaggaagaaa aaaaacagta ttcttatatg cgcataacca
    11041 tgcatgtaaa taccatgttt accgtgctag tgaaatctac gtatggcgtg gacagacgcc
    11101 attcgtgatg tcgatagctg ccacaaggca acggtcttct caccgtagac ccaggcattg
    11161 cgcgccgtga atcttcatga tttcggtcta tcgtgacggg tagcgactct gaacagtgat
    11221 gtttcagggt cagacaggag tagggaagga atacagagag acaataataa tggtagatag
    11281 caagaagcgc cctggcaaag atctcgaccg tatcgatcgt aacattctta atgagttgca
    11341 aaaggatggg cgtatttcta acgtcgagct ttctaaacgt gtgggacttt ccccaacgcc
    11401 gtgccttgag cgtgtgcgtc ggctggaaag acaagggttt attcagggct atacggcgct
    11461 gcttaacccc cattatctgg atgcatcact tctggtattc gttgagatta ctctgaatcg
    11521 tggcgcaccg gatgtgtttg aacaattcaa taccgctgta caaaaacttg aagaaattca
    11581 ggagtgtcat ttagtatctg gtgatttcga ctacctgttg aaaacacgcg tgccggatat
    11641 gtcagcttac cgtaagttgc tgggggaaac cctgctgcgt ctgcctggcg tcaatgacac
    11701 acggacatac gttgttatgg aagaagtcaa gcagagtaat cgtctggtta ttaagacgcg
    11761 ctaacacgga acaggtgcaa aatcggcgta ttttgattac actcctgtta atccatacag
    11821 caacagtact ggggtaacct ggtactgttg tccgttttag catcgggcag gaaaagcctg
    11881 taacctggag agcctttctt gagccaggaa tacactgaag acaaagaagt cacattgaca
    11941 aagttaagta gcggccgtcg ccttctggaa gcgttgctga tccttattgt cctgtttgcc
    12001 gtctggttga tggctgcctt actaagcttt aacccttcgg accccagctg gtcgcaaacg
    12061 gcctggcatg aacctatcca taatttaggt gggatgcccg gtgcgtggct ggcagatacg
    12121 ctgttcttta tttttggcgt gatggcttac accattcccg tcattattgt cggcggttgt
    12181 tggtttgcct ggcgtcatca gtccagtgac gaatacattg attattttgc cgtttcgcta
    12241 cgcattattg gcgttttggc gctcatcctt acctcctgtg gtctggcggc aatcaacgct
    12301 gacgatatct ggtattttgc ctccggtggc gtcattggca gcttactaag cactacgcta
    12361 caaccactgc tacacagtag cgggggaact attgcgctgc tctgcgtttg ggctgcgggc
    12421 ctgacgctgt tcaccggttg gtcatgggtg accattgctg aaaaactcgg cggctggatt
    12481 ttaaacattc tcaccttcgc cagtaatcgt acccgtcgcg atgatacctg ggtcgatgaa
    12541 gatgaatatg aagatgacga agagtatgaa gatgaaaacc acggcaaaca gcatgaatca
    12601 cgccgtgccc gtattcttcg cggcgcgcta gcgcgtcgta aacggttggc ggaaaaattc
    12661 attaatccga tggggcggca aacagacgct gcgttgttct ccggtaagcg gatggatgat
    12721 gacgaagaga ttatctacac tgcacgcggt gtggctgccg acccggacga cgtcctattt
    12781 tcgggcaatc gtgcaacgca gccagaatat gacgaatacg atccattatt aaacggtgcg
    12841 ccaattaccg aacctgtcgc tgtagcagct gctgctacca cggcgacaca aagctgggct
    12901 gcgccggttg aacctgtgac tcagacgccg cctgttgcct ctgttgatgt tccaccttcg
    12961 caacctacag tagcctggca gcctgtaccg ggtccacaaa cgggagagcc ggttattgct
    13021 cctgcaccgg aaggttaccc acagcagtca caatatgcgc agcctgcagt gcaatataat
    13081 gagccgctgc aacaaccagt acagccgcag cagccgtatt atgcacctgc agctgaacaa
    13141 cctgcgcaac aaccttacta cgctcctgcg gcggaacaac ctgttcagca accgtattac
    13201 gcccctgcgc cagaacaacc ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc
    13261 acttttgctc cacagtctac ataccagact gagcaaactt atcagcaacc agccgctcag
    13321 gagccgttgt accaacagcc gcaatccgtt gaacagcagc ctgttgtgga gcctgaaccc
    13381 gttgtagaag agacaaaacc cgcgcgtccg ccgctttact attttgaaga agtggaagag
    13441 aagcgagccc gtgaacgtga acaacttgcg gcctggtatc aaccgattcc agaaccggtt
    13501 aaagaaccag aaccgatcaa atcttcgttg aaagcgcctt ctgttgcagc agtacctcca
    13561 gtagaagccg ctgccgctgt ttctccgctg gcatctggcg tgaaaaaagc gacactggcg
    13621 acgggggctg ccgcaaccgt tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg
    13681 cgtccgcaag tcaaagaggg gattggcccg cagttgccac gaccgaaacg tatccgcgtg
    13741 ccaactcgtc gtgaactggc gtcttacggt attaagctgc cctcacagcg tgcggcggaa
    13801 gagaaagccc gtgaagccca gcgcaatcag tatgattctg gcgatcagta taacgatgat
    13861 gaaatcgatg cgatgcagca ggatgaactg gcacgtcagt tcgcccagac acagcagcaa
    13921 cgctatggcg aacagtatca acatgatgtg cccgtaaacg cagaagatgc agatgctgcg
    13981 gcagaggctg aactggctcg tcagtttgcg caaactcaac aacaacgtta ttccggcgaa
    14041 caaccggctg gggcgaatcc gttctcgctg gatgattttg aattttcgcc aatgaaagcg
    14101 ttgctggatg atggtccaca cgaaccgttg tttacgccaa ttgttgaacc tgtacagcag
    14161 ccgcaacaac cggttgcgcc acagccgcaa tatcagcagc cgcaacaacc agttgcgccg
    14221 cagcagcagt atcagcagcc gcaacaaccg gttgcaccgc ggcagcagga taccctgctt
    14281 catccgctgt tgatgcgtaa tggcgacagc cgtccgttgc ataaaccgac gacgccgctg
    14341 ccttctctgg atttgctgac accgccgccg agcgaagtgg agccggtaga tacctttgcg
    14401 cttgaacaaa tggctcgtct ggtggaagcg cgtctggctg atttccgtat taaagccgat
    14461 gtcgtcaatt actctccggg gccggttatc actcgctttg aacttaacct ggctcctggc
    14521 gtaaaagcgg cgcgcatttc taacttgtca cgggaccttg cccgttcact ttcgacggtg
    14581 gcggtgcgtg tcgttgaagt tattcctggc aaaccctatg taggtctgga gttaccaaat
    14641 aaaaaacgac aaaccgttta tctgcgcgaa gttttggata acgccaaatt ccgcgataat
    14701 ccgtcgccat taaccgtggt gctgggtaaa gatatcgccg gtgagccggt ggttgccgat
    14761 ctggcgaaaa tgccgcactt gttggttgcg gggactaccg gttccggtaa atctgtcggt
    14821 gtgaacgcga tgatcctgag catgctttat aaagcacagc cagaagatgt gcgtttcatc
    14881 atgatcgacc cgaaaatgct ggagctttcg gtgtatgaag gcattccgca cctgttaacg
    14941 gaagtcgtta ctgatatgaa agatgccgcc aacgcgctgc gctggtgtgt taacgagatg
    15001 gagcgtcggt ataaactgat gtctgcgctg ggtgtgcgta atctggcggg ttataacgaa
    15061 aaaattgctg aagccgatcg catgatgcgt ccgattccag acccgtactg gaagccgggt
    15121 gacagtatgg atgcccagca tccggtgctg aaaaaagaac catacattgt ggtgttggtt
    15181 gacgaatttg ccgacctgat gatgacggta ggtaaaaaag tggaagagct gatagcacgt
    15241 ctggcgcaaa aagcccgtgc cgcgggtatc cacctcgtac tggcaactca gcgtccatcg
    15301 gttgatgtta ttactggtct gattaaagcg aatattccga cccgtatcgc ctttaccgta
    15361 tccagtaaga ttgactcacg taccattctt gatcaggctg gcgcggaatc actgctgggt
    15421 atgggggata tgctctactc tgggccgaac tccacgttgc cggtacgtgt ccatggtgct
    15481 tttgttcgcg atcaggaagt tcatgccgtg gtgcaggact ggaaagcgcg tggtcgccca
    15541 cagtatgttg atggcatcac ctccgacagc gaaagcgaag gtggtgcggg tggtttcgat
    15601 ggcgctgaag aactggatcc gttgttcgat caggcggtgc agtttgtcac tgaaaaacgc
    15661 aaagcgtcaa tttctggcgt acagcgtcag ttccgcattg gttataaccg tgcagcacgt
    15721 attatcgaac agatggaagc gcaggggatt gtcagcgaac aggggcacaa cggtaatcgt
    15781 gaagtgctgg ccccaccgcc gtttgactaa ttaatgcatt gccggataag gcgcggtagc
    15841 gtcgcatccg gcactctatc aactgaaaat tcagtatttt cttctttcct caagctgatt
    15901 attagcctgg aatagagagt agagggaact cccgatcggg agtgacgtaa tttgaggaat
    15961 aatgatgaaa aaaattgcca tcacctgtgc attactctca agcttagtag caagcagcgt
    16021 ttgggctgat gccgcaagcg atctgaaaag ccgcctggat aaagtcagca gcttccacgc
    16081 cagcttcaca caaaaagtga ctgacggtag cggcgcggcg gtgcaggaag gtcagggcga
    16141 tctgtgggtg aaacgtccaa acttattcaa ctggcatatg acacaacctg atgaaagcat
    16201 tctggtttct gacggtaaaa cactgtggtt ctataacccg ttcgttgagc aagctacggc
    16261 aacctggctg aaagatgcca ccggtaatac gccgtttatg ctgattgccc gcaaccagtc
    16321 cagcgactgg cagcagtaca atatcaaaca gaatggcgat gactttgtcc tgacgccgaa
    16381 agccagcaat ggcaatctga agcagttcac cattaacgtg ggacgtgatg gcacaatcca
    16441 tcagtttacc gcggtggagc aggacgatca gcgcagcagt tatcaactga aatcccagca
    16501 aaatggggct gtggatgcag cgaaatttac cttcaccccg ccgcaaggcg tcacggtaga
    16561 tgatcaacgt aagtagaggc acctgagtga gcaatctgtc gctcgatttt tcggataata
    16621 cttttcaacc tctggccgcg cgtatgcggc cagaaaattt agcacagtat atcggccagc
    16681 aacatttgct ggctgcgggg aagccgttgc cgcgcgctat cgaagccggg catttacatt
    16741 ctatgatcct ctgggggccg ccgggtaccg gcaaaacaac cctcgctgaa gtgattgccc
    16801 gctatgcgaa cgctgatgtg gaacgtattt ctgccgtcac ctctggcgtg aaagagattc
    16861 gcgaggcgat cgagcgcgcc cggcaaaacc gcaatgcagg tcgccgcact attctttttg
    16921 ttgacgaagt gcaccgattc aacaaaagcc agcaggatgc atttctgcca catattgaag
    16981 acggcaccat cacttttatt ggcgcaacca ctgaaaaccc gtcgtttgag cttaattcgg
    17041 cactgctttc ccgtgcccgt gtttatctgt tgaaatccct gagtacagag gatattgagc
    17101 aagtactaac tcaggcgatg gaagacaaaa cccgtggcta tggtggtcag gatattgttc
    17161 tgccagatga aacacgacgt gccattgcag aactggtgaa tggcgacgcg cggcgggcgt
    17221 taaatacgct ggaaatgatg gcggatatgg ccgaagtcga tgatcgcggt aagcgggtcc
    17281 tgaagcctga attactgacc gaaatcgccg gtgaacgtag cgcccgcttt gataacaaag
    17341 gcgatcgctt ttacgatctc atttccgcac tgcataagtc ggtacgtggt agcgcacccg
    17401 atgcggcgct gtactggtat gcgcgaatta ttaccgctgg tggcgatccg ttatatgtcg
    17461 cgcgtcgctg tttggcgata gcgtctgaag atgtgggtaa cgctgatcca cgagcgatgc
    17521 aggtggcaat tgcggcctgg gattgcttta ctcgcgttgg cccggcggaa ggtgaacgcg
    17581 ccattgctca ggcgattgtt tacctggcct gcgcgccaaa aagcaatgct gtctacactg
    17641 cgtttaaagc cgcgctggcc gatgctcgcg aacgcccgga ttatgacgta ccggttcatt
    17701 tgcgtaatgc gccgacgaaa ttaatgaagg aaatgggcta tgggcaggaa tatcgttacg
    17761 ctcatgatga agcaaacgct tatgctgccg gtgaggttta cttcccgccg gaaatagcac
    17821 aaacacgcta ttatttcccg acaaacaggg gccttgaagg caagattggc gaaaagctcg
    17881 cctggctggc tgaacaggat caaaatagcc ccataaaacg ctaccgttaa tgttatcgtt
    17941 gcggtaatgt tgttactgta tccctgtggt cgcaggctgt ggccacatct cccatttaat
    18001 tcgataagca caggataagc atgctcgatc ccaatctgct gcgtaatgag ccagacgcag
    18061 tcgctgaaaa actggcacgc cggggcttta agctggatgt agataagctg ggcgctcttg
    18121 aagagcgtcg taaagtattg caggtcaaaa cggaaaacct gcaagcggag cgtaactccc
    18181 gatcgaaatc cattggccag gcgaaagcgc gcggggaaga tatcgagcct ttacgtctgg
    18241 aagtgaacaa actgggcgaa gagctggatg cagcaaaagc cgagctggat gctttacagg
    18301 ctgaaattcg cgatatcgcg ctaaccatcc ctaacttgcc tgcagatgaa gtgccggtag
    18361 gtaaagacga aaatgacaac gttgaagtca gccgctgggg caccccgcgt gagtttgact
    18421 ttgaagttcg tgaccatgtg acgctgggtg aaatgcactc tggcctcgac tttgcagctg
    18481 cagttaagct gactggttcc cgctttgtgg taatgaaagg gcagattgct cgcatgcacc
    18541 gcgcactgtc gcagtttatg ctggatctgc ataccgaaca gcatggctac agtgagaact
    18601 atgttccgta cctggttaac caggacacgc tgtacggtac gggtcagctg ccgaaatttg
    18661 ctggcgatct gtttcatact cgtccgctgg aagaagaagc agacaccagt aactatgcgc
    18721 tgatcccaac ggcagaagtt ccgctgacta acctggtgcg cggtgaaatc atcgatgaag
    18781 atgatctgcc aattaagatg accgcccaca ccccatgctt ccgttctgaa gccggttcat
    18841 atggtcgtga cacccgtggt ctgatccgta tgcaccagtt cgacaaagtt gaaatggtgc
    18901 agatcgtgcg cccggaagac tcaatggcgg cgctggaaga gatgactggt catgcagaaa
    18961 aagtcctgca gttgttgggc ctgccgtacc gtaaaatcat cctttgcact ggcgacatgg
    19021 gctttggcgc ttgcaaaact tacgacctgg aagtatggat cccggcacag aacacctacc
    19081 gtgagatctc ttcctgctcc aacgtttggg atttccaggc gcgtcgtatg caggcacgct
    19141 gccgcagcaa gtcggacaag aaaacccgtc tggttcatac cctgaacggt tctggactgg
    19201 ctgttggtcg tacgctggtt gcagtaatgg aaaactatca gcaggctgat ggtcgtattg
    19261 aagtaccaga agttctgcgt ccgtatatga acggactgga atatattggc taatacccaa
    19321 tttttctgaa tctaaaaagc
//
LOCUS       DAZGSP010000095        19143 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZGSP010000095 DAZGSP010000000
VERSION     DAZGSP010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.095"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(36..412)
                     /gene="rnpB"
                     /locus_tag="ACHK74_003772"
     ncRNA           complement(36..412)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHK74_003772"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(445..1590)
                     /gene="garK"
                     /locus_tag="ACHK74_003773"
     CDS             complement(445..1590)
                     /gene="garK"
                     /locus_tag="ACHK74_003773"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFP0222515.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISDLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1687..2577)
                     /gene="garR"
                     /locus_tag="ACHK74_003774"
     CDS             complement(1687..2577)
                     /gene="garR"
                     /locus_tag="ACHK74_003774"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP0222516.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2607..3377)
                     /gene="garL"
                     /locus_tag="ACHK74_003775"
     CDS             complement(2607..3377)
                     /gene="garL"
                     /locus_tag="ACHK74_003775"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFP0222517.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     IEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3393..4727)
                     /gene="garP"
                     /locus_tag="ACHK74_003776"
     CDS             complement(3393..4727)
                     /gene="garP"
                     /locus_tag="ACHK74_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFP0222518.1"
                     /translation="MILDTVDVKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVSLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5102..6673
                     /gene="garD"
                     /locus_tag="ACHK74_003777"
     CDS             5102..6673
                     /gene="garD"
                     /locus_tag="ACHK74_003777"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFP0222519.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQDIPLENASIVSLQDEKHVGFQSMVEDILQIAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNDEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6822..7157
                     /gene="prlF"
                     /locus_tag="ACHK74_003778"
     CDS             6822..7157
                     /gene="prlF"
                     /locus_tag="ACHK74_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFP0222520.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7157..7621
                     /gene="yhaV"
                     /locus_tag="ACHK74_003779"
     CDS             7157..7621
                     /gene="yhaV"
                     /locus_tag="ACHK74_003779"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFP0222521.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(7676..8485)
                     /gene="agaR"
                     /locus_tag="ACHK74_003780"
     CDS             complement(7676..8485)
                     /gene="agaR"
                     /locus_tag="ACHK74_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFP0222522.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            8734..10014
                     /gene="kbaZ"
                     /locus_tag="ACHK74_003781"
     CDS             8734..10014
                     /gene="kbaZ"
                     /locus_tag="ACHK74_003781"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFP0222523.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYYTACEGQ"
     gene            10037..10510
                     /gene="agaV"
                     /locus_tag="ACHK74_003782"
     CDS             10037..10510
                     /gene="agaV"
                     /locus_tag="ACHK74_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFP0222524.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10521..11300
                     /gene="agaW"
                     /locus_tag="ACHK74_003783"
     CDS             10521..11300
                     /gene="agaW"
                     /locus_tag="ACHK74_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFP0222525.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11290..12168
                     /gene="agaE"
                     /locus_tag="ACHK74_003784"
     CDS             11290..12168
                     /gene="agaE"
                     /locus_tag="ACHK74_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFP0222526.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12186..12620
                     /gene="agaF"
                     /locus_tag="ACHK74_003785"
     CDS             12186..12620
                     /gene="agaF"
                     /locus_tag="ACHK74_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFP0222527.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12617..13750
                     /gene="nagA"
                     /locus_tag="ACHK74_003786"
     CDS             12617..13750
                     /gene="nagA"
                     /locus_tag="ACHK74_003786"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP0222528.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNSIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            14100..15254
                     /locus_tag="ACHK74_003787"
     CDS             14100..15254
                     /locus_tag="ACHK74_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFP0222529.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15267..16127
                     /gene="kbaY"
                     /locus_tag="ACHK74_003788"
     CDS             15267..16127
                     /gene="kbaY"
                     /locus_tag="ACHK74_003788"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFP0222530.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16294..16770
                     /gene="agaB"
                     /locus_tag="ACHK74_003789"
     CDS             16294..16770
                     /gene="agaB"
                     /locus_tag="ACHK74_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFP0222531.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            16809..17612
                     /gene="agaC"
                     /locus_tag="ACHK74_003790"
     CDS             16809..17612
                     /gene="agaC"
                     /locus_tag="ACHK74_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFP0222532.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            17602..18393
                     /gene="agaD"
                     /locus_tag="ACHK74_003791"
     CDS             17602..18393
                     /gene="agaD"
                     /locus_tag="ACHK74_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFP0222533.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18394..>19143
                     /locus_tag="ACHK74_003792"
     CDS             18394..>19143
                     /locus_tag="ACHK74_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFP0222534.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLIN"
BASE COUNT         4613 a         4999 c         4912 g         4619 t
ORIGIN      
        1 gcaaaaaccc gccgaagcgg gtttttacgt aaaacaggtg aaactgaccg ataagccggg
       61 ttctgtcgtg gacagtcatt catctaggcc agcaatcgct cactggctca agcagcctac
      121 ccgggttcag tacgggccgt accttatgaa cccctatttg gccttgctcc gggtggagtt
      181 taccgtgcca cggactgtta ccagccgcgc ggtgcgctct taccgcaccc tttcaccctt
      241 acctgatccc gcttgcgcgg gccatcggcg gtttgctctc tgttgcactg gtcgtgggtt
      301 tcccccccag gcgttacctg gcaccctgcc ctatggagcc cggactttcc tcccctccgc
      361 ccgtctcccc cgaagaggac gacgacgaag cggcgactgt ctggtcagct tcggcgcgca
      421 gtatagaggg tttgcgcgcc cttgtcaccc cgcgttgcgc attccaatcg ccagtgtcgc
      481 ggcaatatta cgtgaagcac ggcagatatt gtcataagcc ccgcggaatg cttcgtccaa
      541 cgtacctatg ctggtcaata cgctgaagac cgcatcaatg ccatgctgat gtacaacgcc
      601 aacatcatcg gtcaggctac ccgcaatgcc aatcaccggt ttatggtact tcttcgccac
      661 gtttgcgacg ccaatcggta ccttgccgtg aatactctgg ctgtcaatac gcccttcacc
      721 ggtgatcacc agcgtacaat cgtgaatatg ttcctccaga ttcagcgccg tagtgacgat
      781 ttcaatacca cttttcagtt ccgcaccaag aaacgccatc agcgccgcgc ccataccgcc
      841 agcagctcct gcgccgggga catctttcac atcaacatgc agcgcttttt taatgacatc
      901 ggcatagtga gagaggttat tgtccagctc aacaatcatc gcttcactgg ctcccttttg
      961 tgggccaaag atgcgcgatg cgccgttatc gcccaccagc ggattggtga catcacaagc
     1021 gacgcgaatg acgcaatcct ttaagcgcgg atcgaggtcg gaaatatcaa tatcattcag
     1081 agtattaaga ctaccgccgc caaaaccaat ttcattgccg ttggcgtcgc ataatttcgc
     1141 ccccagcgcc tgtaccatgc ctgcgccgcc atcatttgta gcgctgccgc caatgccgat
     1201 aataatgttt gtcgcgccgc tctccagcgc ctgcaggatt aactcgcctg tgccgcgtga
     1261 agtggtcacg agtggatcgc gtttttccgc aggtaccagc tccagcccac tggccgccgc
     1321 catttcaata aacgcggttt tgccatcgcc ggagatcccc caactggcat tcactttctc
     1381 gcccagcggc cctgtaaccc aggcgtgacg ttcagccccc tgggtggctg caatcatcgc
     1441 ttccaccgtt ccttcgccac cgtcggcaac aggaacagaa acgtactgtg catcaggaaa
     1501 aatttcccga aatccttttt ctatcgcctg cgcaacctcg ctggcagata aactttcttt
     1561 ataagagtct ggggcgatta cgattttcat acctatgcct gttaccacat gacgccggag
     1621 ggcgtttctc ttattcggcc tggattccag gcccggattg caatacgcca tccgggcacg
     1681 acgtcattaa cgagtaactt cgactttcgc cagtttttcg tagtagcacg ccagggcgct
     1741 atgatccgcc gttcctaaac catctgcacg cagtgcctgc atcatctcca taaccgcagc
     1801 tgtgagcggc agttgtgcgc cgacgccgtg ggaagtatcc agcgcatttg ccagatcctt
     1861 aatatgcaga tcaatacgga agcccggctt gaagttgcgg tccatcacca tcggcgcttt
     1921 ggcatccagc acggtactgc ccgccagtcc accgcgaatt gcctgataca ccaggtccgg
     1981 gttaacgccc gctttggttg ccagcgttag cgcttctgac atcgcggcaa tattcagcgc
     2041 cacaatgacc tgatttgcca gtttggtgac gttacctgca ccgatttccc cggtatgcac
     2101 cacggaaccc gccatcgctt tcatcaaatc atagtatttg tcgaaaatag ccttgtcgcc
     2161 gcccaccatc actgacagcg taccgtcgat ggctttcggt tcaccgccgc tcaccggagc
     2221 atccagcata tcaatgcctt tcgctttcag cgcttcgctg atttcacggc ttgccagcgg
     2281 tgcgatagaa ctcatatcga tcaataccgt acctggcttc gcgccttcaa tgatgccatt
     2341 ctcacccagc gccacctctt tcacatgagg ggagtttggc agcatggtta tgatgacgtc
     2401 gcactgttca gcgatcgctt tagccgtaga cgctgtttct gcacctgcag caatcacgtc
     2461 agcaatagct tctgggttac ggtcagcaac caccagcgag taacctgctt tcagaaggtt
     2521 tttactcatt ggtttaccca taatccccag gccaataaaa ccaactttca tagtcatatc
     2581 aatcatctct cttgttgcgg tggtggttat tttttaaagg tatcagccag tttctgagtg
     2641 gcagagcgga agacgccgag atcgctgccg acagccacaa acgtcgcgcc ccattccaga
     2701 taacgacgcg catcggcttc gatcggtgcg aggataccgc tgggtttgcc gtgcgcactg
     2761 gcacggttaa aaatgtgctg aattgctttt tgtacatccg ggtgcgaggc attgccgaga
     2821 tggcctaatg ccgcagccag atcgctgggg ccgacgaaga tgccgtctac gccttcggta
     2881 gcggcaatgg catcgacatt atctacgccc tgctgacttt ctatctggac cagaatagtg
     2941 atgttcttgt tcgactgagc gaaataatcc gccacggtgc caaacatatt ggcgcggtga
     3001 gaaacggaga cgccgcgaat gccttccggc gggtaacgag ttgatgccac cgcctgctct
     3061 gcttcctctt ttgtttctac aaaaggaatc aggaagttat agaaaccgat atccagaaga
     3121 cgcttaataa ttaccggctc gttggtcggc actcgcacta ctggcgcgct ggcgctgcct
     3181 ttcagggcca ttaactgcgg aataaacgtg gaaatatcgt ttggcgcatg ttcgccatcc
     3241 agcaccagcc agtcaaaccc agccaaacca agaacttcag tgctaatcgg gttagagagt
     3301 gctgaccagc aaccaatttg tacctgtttc gcagccagtg cggctttgaa tttattcggg
     3361 aaaacatcgt tattcatcgc ttataccttt gcttatttct gcaattccat acgtttaatg
     3421 tcgccaacta cgaagaggta gcagaccatc gccatcagcg ctgaacatcc cacgaaaacc
     3481 agtgctgcat tgaaggagtg cagttcactt accaggtagc caatcaccag tggagtgaca
     3541 atggaggcaa cattgccaaa gacgttaaag acgccgccac agaggctaac aatctctttc
     3601 ggcgcggtgt cagaaatcac cggccagccc agagcaccaa atcctttgcc aaaaaaagcc
     3661 agcgccatca gcatgaccac cagcgtggtg ttgttggtgt agttacataa gatgatggtg
     3721 gaagccagca acattcccag cacaatcggt agcttacgtg ccagggtcag ggataaaccg
     3781 cgtttgatca gataatccga gaagacacct cccagcacgc cgcccgcaaa accacacagt
     3841 gccggaatcg aggcgaccag acccactttc agaatcgaca tgcctttttc ctgcaccaga
     3901 taaatcggga accaggtgag gaagaaccag gtgatggtgt tgataaaata ttgtccgaaa
     3961 aatacgccca acatcatgcg gttagagagc aattgcttga tgtaatgcag tttgggaccg
     4021 cttgctgccg cgctgcccgg ctttttgtgg tccatatcga ccaccgcgcc attttcagag
     4081 ataaacttca gctcttccgc agacatacgt gggtgatctg tcgggttatg aatcaacttg
     4141 atccacagcg ccgtcagcac aaaaccaatc ccccccataa cggtgaagac gtgctcccag
     4201 ccccaggcga aagtcagcca gccaagcagc ggcgaaaaga gcgccagcga gaaatattgc
     4261 gccgagttaa agatggcgga ggcagtacca cgttcttttg tcggaaacca ggcggcgaca
     4321 atacgggcgt tagccgggaa tgatggcgct tccgagaacc ccagcataaa gcgcataaag
     4381 aacatggaga tccctgccca ggccagcggg aacatatcaa caaagccttg caggaaggtg
     4441 aacagcgacc agaaaaagag gctgtaggtg taaacttttt tcgagccaaa cttatcaagc
     4501 agccagccgc cgggaatttg catcagcagg taggcccagc caaaagcgga gaagatgtaa
     4561 cccatcgaaa ccgcacttaa ctgcaactct tttgccactt cggtaccagc aatagacagc
     4621 gttgcacgat cggcgtagtt aacggcggta acaataaaaa taatcagtaa tattaaatag
     4681 cgggtatgca cgcctttctt ttttacgtca acggtgtcca gaatcatttt atttacctcg
     4741 ggtacttatg ctgattttta ttattatggt gaaggtgtta tttatgagtt ttatttatgc
     4801 cgttacgaca atgaactcgg gaattagtat aagcagcgcg agaataataa tcattgtgca
     4861 aatgctaatt taattaatac tatttaaata ttattttgag catatgcaca taaggttgcg
     4921 cgctaaagca cagatttgcg ctttaccttg ccgggcggca ctgcaatccc tgaaatgatt
     4981 gacattgatc acatttctgc gtttaaactc ctgacattct tatttcaccc aatgaagtca
     5041 tttattttta aatgagacca ggtcctcatt ttaataaccc ctggctggag aatattgcac
     5101 aatggccaac atcgaaatca gacaagaaac gccaactgcg ttttatataa aagttcacga
     5161 cacagataat gtggcaatta ttgttaatga taatggcttg aaagcaggaa cgcgttttcc
     5221 ggatgggctg gaattaattg aacatattcc ccaggggcat aaagtcgcat tgctggacat
     5281 tccggctaat ggtgaaatta ttcgttatgg cgaagtgatt ggttacgccg tgcgcgcaat
     5341 cccacgcgga agctggatcg atgaatcaat ggttgtacta ccggaagcgc cgccgttaca
     5401 cacgctgcca ctggcaacca aagtgccgga acccttaccg ccgcttgaag gttacacctt
     5461 tgaaggctat cgcaatgccg atggcagcgt gggcaccaaa aacctgctcg gcatcaccac
     5521 cagcgtccac tgtgtggcag gcgtggtgga ctacgtagta aaaatcattg aacgcgatct
     5581 gctgccgaaa tacccgaacg tcgatggcgt ggtggggctg aatcatctgt acggttgtgg
     5641 cgtggcgatt aacgctccgg cggcggtggt gcccattcgg accattcaca acatttcgct
     5701 taacccaaac tttggcggcg aggtgatggt gattggcctg ggttgcgaga aattacagcc
     5761 agaacgcctg ctggtcggca cggatgatgt acaagatatt ccgctggaga acgccagtat
     5821 tgtcagcttg caggatgaaa agcatgtcgg ttttcagtcc atggtcgagg atattttgca
     5881 gatcgccgaa cgccatctac aaaaactgaa tcaacggcag cgagaaacct gcccggcttc
     5941 agaactggtc gttggtatgc agtgcggtgg cagcgatgcg ttttctggtg taacggcaaa
     6001 cccggcggtt ggctatgcgt ctgatctact ggtgcgctgc ggcgcaacgg tgatgttttc
     6061 agaagtaacg gaagtgcgtg acgcgatcca tctgctgaca ccacgcgcag tgaacgacga
     6121 ggtcggcaaa cggctgctgg aggagatgga gtggtacgat aactatctca atatgggaaa
     6181 aaccgaccgc agcgccaacc cttcgccggg caacaagaaa ggcggtctgg caaatgtggt
     6241 ggagaaggca ctcggctcca ttgctaaatc gggtaaaagt gcaattgttg aagtgctgtc
     6301 gcccggtcaa cgcccgacta aacgcggatt aatttacgcc gcgacgccag ccagcgattt
     6361 tgtctgtggc acgcaacagg tggcttcggg tatcaccgta caagtgttta cgaccggccg
     6421 tggtacgccg tacggcctga tggcggtacc cgtcattaaa atggcaaccc gcaccgagct
     6481 ggcgaaccgc tggtttgatt taatggatat taacgcgggc actatcgcta ccggcgaaga
     6541 gagcattgaa gaggtgggct ggaagttgtt ccactttatt ctcgacgtcg ccagcgggaa
     6601 gaagaaaacc ttctcggatc aatgggggct gcataaccag ctggcggtgt ttaacccggc
     6661 accggtgacc tgatttcttt tggaacgtcc ctcgcaaaac atggccttag tgccatgttt
     6721 ttattgttta aagcccccac gtccattaat aatgcatttg cattacctta gttcaagctt
     6781 ataatttgag cagaaaacag gctgtaaaag gacagtgaat catgcccgct aatgctcgct
     6841 ctcacgctgt actgaccact gaatcaaagg tcacgatacg cggacaaaca actatccccg
     6901 cgccagtgcg tgaggcctta aaactgaagc caggccagga cagcattcat tacgaaattc
     6961 tgcctggtgg gcaagtattt atgtgccgac tgggagatga acaggaggat catactatga
     7021 atgcattttt gcgttttctg gatacagata tccagaacaa cccgcaaaaa actcgtccat
     7081 tcaacattca acaaggaaag aaacttgtcg ctggcatgga cgtcaacatt gatgatgaga
     7141 ttggcgacga cgaataatgg attttccaca aagggttaat ggttgggcgc tatatgctca
     7201 tccctgtttt caggaaacct acgacgcttt agttgccgaa gtcgagacat taaagggaaa
     7261 agatcctgaa aattatcaga gaaaagccgc cacaaagtta ttggcggtag tccataaagt
     7321 gattgaggag catatcacgg tcaatccatc atcaccggca ttccgtcatg gcaagtcgtt
     7381 aggctctggg aaaaataaag actggtcacg ggtaaaattt ggtgctggtc gttatcgtct
     7441 cttctttcgt tatagtgaaa aagagaaagt catcattctg ggatggatga acgatgaaaa
     7501 cactctgcgc acctacggta aaaaaacaga tgcctatacc gtattcagca aaatgttaaa
     7561 aagaggacat cctcctgccg actgggaaag cctcacccaa gagacagaag aaaaccattg
     7621 atggtgttca caatgcccgc cgaacgtatt cgtgttgacc agcgggcacc ctttctcact
     7681 ccccgaccag aatcacttca accccagcct ttcgcagtcc ttccagacta tccgcaggaa
     7741 tgccttcatc aacaatgatc atgtcgatac gttgagtatc aatgatctta tgtaaactgg
     7801 aacgattgaa tttactggaa tcggtgacca cgatgatccg ttccgcaact tcgcacatcc
     7861 gacggtttaa acgggcttca tcttcattat gtgtgctgac gccgcgctcc agatcgatcg
     7921 catctacacc aagaaacagc atatcgaagt ggtaattttg cagcgattgc tcagcctgat
     7981 cgccgtaaaa agattgcgac tgacggcgca aatgcccgcc ggtcatcagc agctcaacgc
     8041 cttccgcttc cagcaacgca ttagccacgt tcataccgtt ggtcatcgca attacgtcag
     8101 tgtgcttacg catcagacga gcaatctcaa aagtggtagt cccggaatcg aggatcaccc
     8161 gatgaccagg ctgaatcaac tcaacggcag ctttcgcaac gctgcgtttc atcgcggtgt
     8221 tcagcgcgct tttatcttcc actgatggct cgactgacgg cgtcgtgcta tcgcagatca
     8281 acgcgccacc ataggcacgc acagcgatcc cctgcttttc cagaaacgcc agatcattgc
     8341 ggatcgtcac agtagatacg ccatacaatg ccgacagatc gttaacctgc acactccctt
     8401 gctgtcgcag acgctgaatg atctgttctc gtcgctcgct ggtgccagtc actcgcttct
     8461 cacctgaagc gtcggtatta ctcatagtaa gtcctttcgt aaaactttcg tttcatttcg
     8521 ttttgcctat taacgccttt ctattaagca aatgcaagcc caccttgccc attggcgcaa
     8581 gctactctcg tttcagtgac tttcattatg tttcttttgt gaatcagatc agaaaactat
     8641 tatctttcgt tttattttta tctcaccatg acgcagtatc aactgaaaca aaacgaaaga
     8701 ttaatatcgc agtaatctga actggagagg aaagtgaaac atctgacaga aatggtgaga
     8761 cagcacaaag cgggcaaaac aaatggaatt tatgccgttt gttccgcaca tccgctggtg
     8821 ctggaggctg caatccgcta cgccagtgca aaccaaacgc cgttactgat tgaagcaacc
     8881 tccaatcagg tagaccagtt cggcggttat accggaatga cgcccgccga ttttcgcggc
     8941 tttgtttgtc agctcgccga ctcgttgaat ttcccgcagg atgcgttgat tctgggtggt
     9001 gaccatctgg ggccaaaccg ctggcaaaac ctgccggccg ctcaggcaat ggccaatgcc
     9061 gatgatttga ttaaaagcta cgttgcggca ggattcaaaa aaatccacct tgattgcagc
     9121 atgtcctgtc aggacgatcc gattccctta actgatgaca tcgtggctga acgcgccgcc
     9181 cgtctggcga aagtggcgga agaaacctgt cttgaacact ttggcgaagc cgatctggag
     9241 tatgtcattg gtaccgaagt gccggtacct ggcggcgcgc atgaaacctt aagcgagctg
     9301 gcggtcacca cgccggatgc cgcccgcgcc acgctggaag cccatcgtca cgcctttgaa
     9361 aagcaaggtt tgaatgccat ctggccacgc atcattgccc tggtggttca acccggcgtc
     9421 gaattcgatc acaccaacgt tattgattat cagcccgcca aagcgagcgc cttaagccag
     9481 atggtcgaaa actacgaaac gctgattttc gaagcgcact ctaccgatta tcaaacgccg
     9541 caatcgctgc gccagctggt gattgaccac tttgccattc tgaaagttgg cccagcgctg
     9601 accttcgccc tgcgtgaagc tctgttctct ctggcggcga ttgaagaaga actggtgcca
     9661 gcgaaagcct gttctggtct gcgtcaggtg ctggaagacg tgatgctcga ccgcccggaa
     9721 tactggcaaa gccactacca cggtgacggc aacgcgcgtc gtctggcgcg tggttatagc
     9781 tactcggatc gcgtgcgcta ttactggccg gacagccaga ttgatgacgc tttcgctcat
     9841 ctggttcgta atctggcgga ttcaccaatt ccgctaccgc tcatcagcca gtatctgccg
     9901 ctgcaatacg tgaaagttcg ctccggcgag ctgcaaccga cgccacggga actcattatc
     9961 aaccatattc aggacatcct ggcgcagtac tacacagcct gtgaaggcca ataagcaaaa
    10021 caaagaggaa cacgctatgc caaatattgt tttaagccgg attgatgaac gcttgattca
    10081 cggtcaggtc ggcgttcagt gggtcggatt tgcgggggca aatctggtgc tggtagccaa
    10141 cgatgaggtt gccgaagatc cggtacaaca aaacctgatg gaaatggtac tggcagaagg
    10201 gatcgccgta cgtttctgga cgctgcaaaa agttatcgac aacattcatc gcgccgccga
    10261 tcgacagaaa atcctgctgg tttgtaaaac acccgccgat ttcctgacgc tggtgaaagg
    10321 tggcgttccg gtgaatcgca ttaacgttgg caatatgcac tacgccaatg gcaaacaaca
    10381 aatcgccaaa acggtttctg tggatgcggg cgatatcgca gcatttaacg acctgaaagc
    10441 cgctggggtg gaatgcttcg ttcagggcgt cccgacagag cctgctgtgg acctctttaa
    10501 attactttga gggattcatc atggaaatca gcctgttgca ggcatttgcg ttgggcatta
    10561 tcgcctttat cgctggcctg gatatgttta acggcctaac acatatgcac cgcccggtgg
    10621 tcctcggccc gttggtcggg ctggtacttg gcgatctgca taccggaatt ttaaccggcg
    10681 gtacgctgga actggtgtgg atggggctgg ccccgctggc gggcgcacag ccgcctaacg
    10741 tgattatcgg tactatcgtc ggcacggcgt ttgccattac tactggcgtg aaacccgatg
    10801 tcgcggtcgg tgtcgccgta cctttcgctg tcgcagtaca gatggggatt accttcctgt
    10861 tctcggtgat gtctggcgtg atgtctcgct gcgaccggat ggcggagaac gccgatactc
    10921 gcggcattga acgcgtgaac tatctggcac tgctggcact cggcactttc tattttctct
    10981 gcgctttcct gcctatctac ttcggcgcgg aacatgcaaa aaccatcatt gatgtcctgc
    11041 cgcaacgatt aatcgacggc ctcggcgtcg cgggcggcat catgccagca atcggttttg
    11101 ccgtgctgct gaaaatcatg atgaaaaacg tctacatccc ctacttcatc ctgggctttg
    11161 ttgccgcagc ctggctcaag ttaccggtgc tggctattgc tgccgctgcg ctggcgatgg
    11221 cgctgatcga cctgctgcgt aaatctcctg aaccgactca acctgcggca cagaaagagg
    11281 aatttgaaga tggcatctaa tcaaaccacc ctgccgaacg tcagtgaaaa cgaagaaaca
    11341 ttgctgactg gcgtcaatga aaacgtgtat gaagatcaga gcattggcgc ggagctgacg
    11401 aaaaaagata tcaaccgcgt cgcctggcgt tccatgctgt tacaagcgtc ttttaactac
    11461 gaacgtatgc aggcttctgg ctggttgtac ggtctgctgc ctgctctgaa aaagatccac
    11521 actaataaac gcgacctggc gcgcgccatg aaggggcata tgggtttctt caatacccat
    11581 ccgtttctgg tgacatttgt tatcggcatt atccttgcga tggagcgttc taagcaggac
    11641 gttaacagta ttcagagcac caaaattgcc gtcggtgcgc cgctcggcgg gattggcgat
    11701 gcaatgttct ggctaacgct actgccgatt tgtggcggga taggcgcaag ccttgcgcta
    11761 caaggctcta ttcttggcgc agtcgtattt attgtgctgt tcaacgtggt acacctgggg
    11821 ctgcgttttg gtctggcgca ttatgcttac cgcatgggcg tggcggcgat tccactcatt
    11881 aaagcgaata ccaaaaaagt cggccatgct gcgtctatcg ttgggatgac ggtaatcggc
    11941 gcgctggtgg caacctatgt tcgtttaagc accacgctgg aaatcacagc gggcgacgca
    12001 gtggttaagt tacagactga tgttattgac aaactgatgc ctgccttctt accgctggtc
    12061 tacaccttga ccatgttctg gctggtacgc cgcggctgga gtccgctgcg tctgattgca
    12121 gttaccgtgg ttctcggcat cgtcggtaaa ttctgccatt tcctttaaat acaatgaggt
    12181 attagatgtt aagtattatt ttgacagggc atggcgggtt tgccagcggc atggaaaaag
    12241 caatgaagca aattctcggt gagcaatcgc agtttatcgc catcgatttt ccggaaacct
    12301 caagcaccgc gctgcttaca tcgcagcttg aagaggccat cgcccaactg gattgtgaag
    12361 acggcattgt ttttctgacg gatttgctgg gcggcacgcc gtttcgcgta gcttcaacgc
    12421 tggcgatgca aaaaccgggc tgtgaagtga tcaccggcac caatttgcaa ctgctactgg
    12481 agatggtgct ggagcgcgaa gggttaagcg gtgaagaatt ccgtgtacag gcgctggagt
    12541 gtggacaccg agggctgacc agtctggtgg acgagttagg ccgctgccat gaagaatgtc
    12601 cggtcgagga aggaatatga cacacgttct gcgcgccaga aggctgctga ctgaagaggg
    12661 atggctcgat gaccatcagt tgcgtattgc tgacggtgtc atcgcagcaa tcgaaccgat
    12721 tccagtgggc gtgactgaac gcgatgcgga actgctctgc cccgcttaca tcgacaccca
    12781 tgtacacggt ggtgcgggcg ttgatgtaat ggatgacgcg ccggatgtac tcgacaagct
    12841 ggcaatgcac aaggcacgcg aaggtgtcgg cagttggttg ccgactaccg taaccgcgcc
    12901 gcttaacagc attcatgcgg cgctgaaacg tattgctcaa cgttgccaac gcggcggacc
    12961 tggtgcgcaa gtgctgggga gttatctcga aggaccgtac ttcacgccgc agaataaagg
    13021 cgcgcatccg ccggaattgt ttcgcgagct tgaaattgcc gagctggatc aattgattgc
    13081 cgtttctcag cacaccttac gcgtggtagc gctggcaccg gaaaaagagg gggcattgca
    13141 ggccatccgc catcttaaac agcaaaatgt acgagtgatg ctggggcata gcgcggcgac
    13201 ctggcaacaa actcgcgccg cgtttgatgc tggtgccgac ggcctggtgc attgctataa
    13261 cgggatgaca ggtttacatc accgcgaacc gggaatggtt ggcgcgggat taacggacaa
    13321 gcgcgcctgg ctggaactga tagccgatgg tcatcatgtg catccggcgg cgatgtcgct
    13381 gtgttgttgc tgtgcaaaag agagaatcgt gctgatcacc gacgcgatgc aggcagccgg
    13441 gatgccggat ggtcgctata cgttatgtgg cgaagaagtg cagatgcacg gtggcgttgt
    13501 ccgtaccgcg tccggtgggc tggcgggcag tacgctgtct gttgatgcgg cagtgcgcaa
    13561 catggtcgag ttgacgggcg taacgcctgc ggaagccatt catatggcat cgctgcatcc
    13621 ggcgcgaatg ctgggtgttg atggtgttct gggatcgctt aaaccgggca aacgcgccag
    13681 cgtcgttgcg ctggatagcg ggctacatgt gcaacaaatc tggattcagg gtcaattagc
    13741 ttcgttttga tggtttgctc cattattggg ccttcacttc ccccgtaagg cctttctttt
    13801 tctttctttt tgatctgtgc agcggtgtcg gatgcgacgc taacgcgtct tatgcgacct
    13861 acggtacgcc gcatccggca atcggtgaac atagcgacgt cattacgcaa ttcttaatct
    13921 tcccttgtct tttctttcaa cgatcacaga tttcgtttta tttccttttt ctccattgaa
    13981 ctttcagttt cttttctata gattttaatc aacgaaagac atcaccaagt gaaatgaaac
    14041 gaaaggcaag tgaaagcgac aacgcccgac gtcaagttca tcagactaag gattgagtta
    14101 tgccagaaaa ttacacccct gctgccgccg caaccggtac atggactgaa gaagagatcc
    14161 gccatcagcc tcgcgcatgg atccgttcac tcaccaacat cgacgcgcta cgttccgcgc
    14221 tcaataactt ccttgaaccg ttactgcgca aagagaatct gcggatcatc ctgaccggag
    14281 ccggaacgtc ggcatttatc ggtgacatca tcgcgccgtg gctcgccagc cataccggta
    14341 aaaacttcag cgccgtaccg accaccgatc tggtcaccaa tccgatggac tacctgaatc
    14401 cagctcatcc gctgctgttg atctccttcg gtcgatccgg caacagcccg gaaagcgtcg
    14461 ctgccgtgga actggcaaat caatttgtac cggaatgcta tcacctgccg atcacctgca
    14521 acgaagcggg cgctctttac caaaacgcga tcaacagcga taacgcattt gccctgctga
    14581 tgcccgcaga aacgcacgat cgcggctttg cgatgaccag cagcattacc accatgatgg
    14641 ccagctgcct cgcggttttc gcacctgaga cgatcaacag ccaaaccttc cgcgacgtgg
    14701 cggatcgttg ccaggcgatc ctgacctcac tgggcgattt cagcgaaggt gtgtttggtt
    14761 acgcaccgtg gaaacggatc gtttatctcg gcagcggtgg cttacagggc gcagcacgcg
    14821 agtcggcgct gaaagtgctg gaactgacgg cgggtaaact ggcggccttt tatgattctc
    14881 caaccggatt ccgtcatgga ccaaaatcgc tggtcgataa cgaaacactg gtggtggtat
    14941 ttgtctccag ccacccttac acccgtcagt atgatcttga tctgctggct gaactccgcc
    15001 gtgacaacca ggcaatgcgc gtaatcgcca tcgccgcgga aagcaccgac atcgtcgctg
    15061 ccggtccaca tattatcctg ccgccgtcac gtcactttat cgacgttgag caggcatttt
    15121 gcttcctgat gtacgcccag acgtttgcac tgatgcagtc gctgcacatg ggcaatacgc
    15181 cggatacccc atcagccagc ggcaccgtta accgcgtggt gcaaggcgta atcattcatc
    15241 cgtggcaggc ataagaggat cgcattatga gcattatctc cactaaatat ctgttgcagg
    15301 acgcccaggc caatggctac gcggtgcctg cttttaacat tcataacgcc gagacgatcc
    15361 aggcgatcct cgaagtgtgc agtgaaatgc gatcgccggt gatcctcgcc ggaacgccgg
    15421 ggacctttaa acatatcgcg ctggaagaga tctacgccct gtgcagcgcc tattccacaa
    15481 cctacaacat gccactggcg ctgcatctcg accaccacga atcgctggat gatattcgcc
    15541 gtaaagtcca cgcaggtgtg cgcagtgcaa tgatcgacgg cagccacttc ccgtttgccg
    15601 agaacgtgaa gctggtgaaa tcggttgttg acttctgcca ctcacaggat tgcagcgtgg
    15661 aagcagaact gggccgcctg ggcggtgttg aagatgacat gagcgttgac gccgaaagtg
    15721 cattcctgac cgatccacaa gaagctaaac gctttgtcga actgactggc gtcgacagcc
    15781 tggcggtagc gattggtacg gcgcacggct tatacagcaa aacgccgaag attgatttcc
    15841 agcggctggc ggaaattcgt gaagtggtgg atgttcctct ggtgctacat ggtgccagcg
    15901 atgttccgga tgaatttgtc cgtcgcacta ttgaacttgg cgtcacaaaa gtgaacgttg
    15961 ccacagaatt aaaaatagcc ttcgctggcg cggttaaagc ctggtttgcg gaaaatccgc
    16021 agggtaatga tcctcgttat tatatgcgcg tcggaatgga tgcgatgaaa gaagttgtca
    16081 gaaataaaat taatgtctgt ggttcagcga atcgaatttc agcataatca ttagtttttt
    16141 atgatttatc ccaatgtact tcccgattaa tatcggggag tgccttaatg gaaaaggaga
    16201 tgactaaacc ttaataaata catcagtaca atatcgcaac aataatatat taaaaaaaat
    16261 tatattattc aactttatgg tgaggattac acaatgagca gtccaaatat tctcttaacc
    16321 cgtattgata accgtctggt tcacggtcag gttggcgtga cctggacatc caccatcggt
    16381 gcgaatctgc tggtagtcgt ggatgatgtt gtcgctaacg atgatattca acagaaatta
    16441 atgggtatta ccgcagaaac ctacggcttt ggcattcgtt tctttactat cgaaaaaacc
    16501 attaacgtca tcggcaaagc tgcaccgcat cagaagatct tcctgatttg ccgtacgcca
    16561 caaacggtac gtaaattggt agaaggtggc attgacctga aagatatcaa cgtcggcaat
    16621 atgcatttct cggaagggaa aaagcaaatc agcagtaaag tttatgtcga tgaccaggat
    16681 ctcgcggact tacgttttat taaacaacgt ggcgtgaatg ttttcattca ggacgtccct
    16741 ggcgatcaaa aagaacaaat ccctgactaa atctaaaatc gccttaatat tggtttgagg
    16801 taataaaaat gcatgaaata accctacttc agggattatc cctggcggcg ttagttttcg
    16861 ttctggggat tgatttttgg ctggaagcct tatttttatt ccgcccgata atcgtttgta
    16921 ccctaactgg cgctattctc ggtgatattc agactggctt aattaccggt ggtctgacag
    16981 agttggcttt cgccggatta acccctgcag gtggtgttca gccgcccaac ccgattatgg
    17041 cgggtctgat gaccaccgtc attgcatggt ctacgggcgt tgatgccaaa acagcaattg
    17101 gtcttggcct gccgtttagt ttgttaatgc agtacgtcat tctgttcttc tattccgctt
    17161 tctcattatt tatgaccaaa gccgataaat gcgcgaaaga ggcggatacg gcagcgtttt
    17221 cccggcttaa ctggacaacg atgctcatcg tcgcttcagc gtatgcggtg attgctttcc
    17281 tctgtactta cctggcacaa ggggcgatgc aggcgctggt gaaagcgatg cccgcctggt
    17341 tgacccacgg ctttgaagtg gctggcggta ttctgcctgc cgttggtttt ggcttgctgc
    17401 tgcgcgtaat gttcaaagcg caatatatcc cttacctgat cgccggtttc ctgtttgttt
    17461 gctacatcca ggtcagcaac ctgttgccgg ttgccgtact gggcgcaggc tttgcggtgt
    17521 atgagttttt caatgcgaaa tcccggcagc aagcgcaacc gcagcccgtt gccagtaaaa
    17581 atgaagaaga ggactacagc aatgggatct gaaatcagta aaaaagatat cacccgtctg
    17641 ggctttcgtt cgtcgctgct gcaagcgagc tttaactacg aaaggatgca ggcgggcggt
    17701 tttacctggg cgatgttgcc gatcctgaaa aagatttata aggacgacaa accgggctta
    17761 agcgcggcga tgaaagataa cctcgaattt attaataccc acccgaatct ggtcggattc
    17821 ctgatggggt tattaatttc gatggaagaa aaaggagaaa accgcgacac cattaaaggc
    17881 ctcaaagtgg cactgtttgg cccaatcgcc gggattggcg atgcgatttt ctggtttact
    17941 ttgttgccga ttatggcggg aatttgctca tcatttgcca gccagggaaa cctgctgggg
    18001 ccgattctat ttttcgccgt ttacctgctt atctttttcc tgcgcgtcgg ctggacccac
    18061 gtcggttatt cagtcggcgt gaaggcgatc gataaagtgc gagagaactc gcagatgatt
    18121 gctcgctcgg caaccatcct cgggatcacg gtaatcggcg ggctgatcgc ttcgtatgtg
    18181 catattaacg tggtgacatc gtttgccatc gacagtaccc acagcgtcgc gctgcaacag
    18241 gatttcttcg ataaagtctt cccgaacatt ttaccgatgg cctacaccct gctgatgtat
    18301 tacttcctgc gggtgaaaaa agcgcatccg gtgctgttaa tcggcgtgac ttttgtgctc
    18361 tctattgttt gttccgcatt cggcattttg taaatggaac gaggcactgc ttctggcggt
    18421 gcctctttac ttaaggaatt tcatcctgtg caaacccttc agcaagttga aaactatacg
    18481 gcgttaagtg aacgagccag cgaatattta ttggctgtga tccgtagcaa accggatgcc
    18541 gtgatttgcc tggcgaccgg agccacgcca ttactgacgt atcattatct ggtagaaaaa
    18601 atccaccagc agcaggttga tatcagccag ctcaccttcg tgaagctcga cgaatgggtg
    18661 gatctgccat taacgatgcc tggcacctgc gaaactttcc tacaacagca tatcgtgcag
    18721 ccgctggggc tgcgtgaaga ccagctcatc agctttcgct ccgaagagat aaatgagaca
    18781 gagtgcgaac gggtaacgaa cctgattgcg cgcaaaggcg gtctggattt atgcgttcta
    18841 ggattgggga aaaacggtca tcttgggctg aacgaaccgg gagaaagcct acaaccggcc
    18901 tgccatatca gtcaacttga tgccagaaca cagcaacatg agatgttaaa aaccacgggt
    18961 cgccccgtga ctcgtgggat caccttaggc ctgaaggata ttctcaatgc ccgcgaggtt
    19021 ttgttattgg tgactggcga aggaaagcag gatgcgacag aacgttttct cacggctaaa
    19081 gtctccaccg ctatcccggc ttcgttttta tggctgcaca gtaactttat ttgtttaatt
    19141 aat
//
LOCUS       DAZGSP010000096        18922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZGSP010000096 DAZGSP010000000
VERSION     DAZGSP010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.096"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            271..837
                     /gene="yqaB"
                     /locus_tag="ACHK74_003793"
     CDS             271..837
                     /gene="yqaB"
                     /locus_tag="ACHK74_003793"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFP0222535.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            834..1261
                     /locus_tag="ACHK74_003794"
                     /pseudo
     CDS             834..1261
                     /locus_tag="ACHK74_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
     gene            1334..2890
                     /gene="gshA"
                     /locus_tag="ACHK74_003795"
     CDS             1334..2890
                     /gene="gshA"
                     /locus_tag="ACHK74_003795"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFP0222536.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            3040..3555
                     /gene="luxS"
                     /locus_tag="ACHK74_003796"
     CDS             3040..3555
                     /gene="luxS"
                     /locus_tag="ACHK74_003796"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFP0222537.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGKGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3619..5157)
                     /gene="emrB"
                     /locus_tag="ACHK74_003797"
     CDS             complement(3619..5157)
                     /gene="emrB"
                     /locus_tag="ACHK74_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFP0222538.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERWRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5174..6346)
                     /gene="emrA"
                     /locus_tag="ACHK74_003798"
     CDS             complement(5174..6346)
                     /gene="emrA"
                     /locus_tag="ACHK74_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFP0222539.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6473..7003)
                     /gene="emrR"
                     /locus_tag="ACHK74_003799"
     CDS             complement(6473..7003)
                     /gene="emrR"
                     /locus_tag="ACHK74_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFP0222540.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(7094..7429)
                     /gene="ygaH"
                     /locus_tag="ACHK74_003800"
     CDS             complement(7094..7429)
                     /gene="ygaH"
                     /locus_tag="ACHK74_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFP0222541.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALTYG
                     LVWKVMAII"
     gene            complement(7419..8156)
                     /gene="ygaZ"
                     /locus_tag="ACHK74_003801"
     CDS             complement(7419..8156)
                     /gene="ygaZ"
                     /locus_tag="ACHK74_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFP0222542.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8280..9464)
                     /locus_tag="ACHK74_003802"
     CDS             complement(8280..9464)
                     /locus_tag="ACHK74_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0222543.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9755..10747)
                     /gene="proX"
                     /locus_tag="ACHK74_003803"
     CDS             complement(9755..10747)
                     /gene="proX"
                     /locus_tag="ACHK74_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFP0222544.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10805..11869)
                     /gene="proW"
                     /locus_tag="ACHK74_003804"
     CDS             complement(10805..11869)
                     /gene="proW"
                     /locus_tag="ACHK74_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFP0222545.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGMATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11862..13064)
                     /gene="proV"
                     /locus_tag="ACHK74_003805"
     CDS             complement(11862..13064)
                     /gene="proV"
                     /locus_tag="ACHK74_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFP0222546.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13418..14377)
                     /gene="nrdF"
                     /locus_tag="ACHK74_003806"
     CDS             complement(13418..14377)
                     /gene="nrdF"
                     /locus_tag="ACHK74_003806"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP0222547.1"
                     /translation="MKLSRISTINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14387..16531)
                     /gene="nrdE"
                     /locus_tag="ACHK74_003807"
     CDS             complement(14387..16531)
                     /gene="nrdE"
                     /locus_tag="ACHK74_003807"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP0222548.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16504..16914)
                     /gene="nrdI"
                     /locus_tag="ACHK74_003808"
     CDS             complement(16504..16914)
                     /gene="nrdI"
                     /locus_tag="ACHK74_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP0222549.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(16911..17156)
                     /gene="nrdH"
                     /locus_tag="ACHK74_003809"
     CDS             complement(16911..17156)
                     /gene="nrdH"
                     /locus_tag="ACHK74_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFP0222550.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17404..17733)
                     /locus_tag="ACHK74_003810"
     CDS             complement(17404..17733)
                     /locus_tag="ACHK74_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFP0222551.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            17885..18229
                     /locus_tag="ACHK74_003811"
     CDS             17885..18229
                     /locus_tag="ACHK74_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFP0222552.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(18266..18715)
                     /gene="alaE"
                     /locus_tag="ACHK74_003812"
     CDS             complement(18266..18715)
                     /gene="alaE"
                     /locus_tag="ACHK74_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFP0222553.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         4757 a         4788 c         4893 g         4484 t
ORIGIN      
        1 aaccttcagc aatgaaggta tcgaagagat agcggtatta accgcaggca ccagggagga
       61 taacgttgcg tcagcaacgg cccgaagggc gaacgccagt gagtaatcct cccggatgca
      121 ccatcttctc ccaaaaattt ccttcaaaaa tatctcattt ctaaataact actttatcca
      181 tttcctgaat tttcttctcc ttcgccatca gcgacaaaat caagcaatta cgctaaagta
      241 gcgtcatatt tttcgacttg cgagaagacc atgtacgagc gttatgcagg tttaattttt
      301 gatatggatg gcacaatcct ggatacggag cctacgcacc gtaaagcgtg gcgcgaagta
      361 ttagggcact acggtcttca gtacgatgtt caggcaatga ttgcgcttaa tggatcgccc
      421 acctggcgta ttgctcaggc aattattgag ctgaatcagg ccgatctcga cccgcatgcg
      481 ttagcgcgtg aaaaaacaga agcagtaaga agtatgctgc tggatagtgt cgagccgctt
      541 cctcttgttg aagtggtgaa aagttggcat ggtcgtcgcc caatggctgt aggaacgggg
      601 agtgaaagcg ccatcgctga ggcattgctg gcgcacctgg gattacgccg ttattttgac
      661 gccgtcgtcg ctgctgatca cgtcaaacac cataaacccg cgccagacac atttttgttg
      721 tgcgcgcagc gtatgggcgt gcaaccgacg cagtgtgtgg tctttgaaga tgccgatttc
      781 ggtattcagg cggcccgtgc agcaggcatg gacgccgtgg atgttcgctt gctgtgagtg
      841 aagcgttatc gcttttctcg ttgtttgcca gtagttttct cagcgctaca ctattacccg
      901 gcaactccga agtcgtgctg gtggcaatgt tgctttccgg gatcagttat ccctgggttt
      961 tagttttaac agcaacaatg ggtaatagcc ttggagggtt aactaacgtt atccttgggc
     1021 gtttctttcc attgcgtaaa acatcgcgct ggcaagagaa agctaccggc tggctgaaac
     1081 gctatggtgc agtcacgcta ttattaagct ggatgcccgt ggttggcgat ttactgtgtc
     1141 tgttagcggg atggatgcgc atctcctggg ggccagtaat ctttttttgt gccttggtaa
     1201 agcgttacgc tatgttgcag ttgcagcagc gaccgttcag ggcatgatgt ggtggcacta
     1261 attgtaggcc tgcacatatg gtcaccatta cagttatgct aattaaaacg attttgacag
     1321 gcgggaggtc aatttgatcc cggacgtatc acaggcgctg gcctggctgg aaaaacatcc
     1381 tcaggcgtta aaggggatac agcgtgggct ggagcgcgaa actttgcgtg ttaatgctga
     1441 tggcacactg gcaacaacag gtcatcctga agcattaggt tccgcactga cgcacaaatg
     1501 gattactacc gattttgcgg aagcattgct ggaattcatt acaccagtgg atggtgatat
     1561 tgaacatatg ctgaccttta tgcgcgatct gcatcgttat acggcgcgca atatgggtga
     1621 tgagcggatg tggccgttaa gtatgccatg ctacatcgca gaaggtcagg acatcgaact
     1681 ggcacagtac ggcacttcta acaccggacg ctttaaaacg ctgtatcgtg aagggctgaa
     1741 aaatcgctac ggcgcgctga tgcaaaccat ttccggcgtg cactacaatt tctctttgcc
     1801 aatggcattc tggcaagcga agtgcggtga tatctcgggc gctgatgcca aagagaaaat
     1861 ttctgcgggc tatttccgcg ttatccgcaa ttactatcgt ttcggttggg tcattcctta
     1921 tctgtttggt gcatctccgg cgatttgttc ttctttcctg caaggaaaac caacgtcgct
     1981 gccgtttgag aaaaccgagt gcggtatgta ttacctgccg tatgcgacct ctcttcgttt
     2041 gagcgatctc ggctatacca ataaatcgca aagcaatctt ggtattacct tcaacgatct
     2101 ttacgagtac gtagcgggcc ttaaacaggc aatcaaaacg ccatcggaag agtacgcgaa
     2161 gattggtatt gagaaagacg gtaagaggct gcaaatcaat agcaacgtgc tgcagattga
     2221 aaacgaactg tatgcgccga ttcgtccaaa acgcgttacc cgcagcggcg agtcaccttc
     2281 tgatgcgctg ttacgtggcg gcattgaata tattgaagtg cgttcgctgg acatcaaccc
     2341 gttctcgccg attggtgtag atgaacagca ggtgcgattc ctcgacctgt ttatggtctg
     2401 gtgtgcgctg gctgatgcac cggaaatgag cagtagcgaa cttgcctgta cgcgcgttaa
     2461 ctggaaccgg gtgatccttg aaggtcgcaa accgggcctg acgctgggta tcggctgcga
     2521 aaccgcgcag ttcccgttac tgcaggtggg taaagatctg ttccgcgatc tgaaacgcgt
     2581 cgcgcaaacg ctggatagca ttaacggcgg cgaagcgtat cagaaagtgt gtgatgaact
     2641 ggttgcctgc ttcgataatc ccgatctgac tttctctgcc cgtatcttaa ggtctatgat
     2701 tgatactggt attggcggaa caggcaaagc gtttgcagaa gcctaccgta atctgctgcg
     2761 tgaagagccg ctggaaattc tgcgcgaaga ggattttgta gccgagcgcg aggcatctga
     2821 acgccgtcag caggaaatgg aaaccgctga taccgaaccg tttgcggtgt ggctggaaaa
     2881 acacgcctga cagaaaagaa aaaggccact cgtgagtggc caaaatttca tctctgaatt
     2941 cagggatgat gataacaaat gcgcgtcttt catatactca gactcgcctg ggaagaaaga
     3001 gttcagaaaa ttttaaaaaa aattaccgga ggtggctaaa tgccgttgtt agatagcttc
     3061 acagtcgatc atacccggat ggaagcgcct gcagttcggg tggcgaaaac aatgaacacc
     3121 ccgcatggcg acgcaatcac cgtgttcgat ctgcgcttct gcgtgccgaa caaagaagtg
     3181 atgccagaaa gagggatcca taccctggag cacctgtttg ctggttttat gcgtaaccat
     3241 cttaacggta agggtgtaga gattatcgat atctcgccaa tgggctgccg caccggtttt
     3301 tatatgagtc tgattggtac gccagatgag cagcgtgttg ctgatgcctg gaaagcggca
     3361 atggaagacg tgctgaaagt gcaggatcag aatcagatcc cggaactgaa tgtctaccag
     3421 tgtggcactt accagatgca ctcgttgcag gaagcgcagg atattgcgcg tagcattctg
     3481 gaacgtgacg tacgcatcaa cagcaacgaa gaactggcac tgccgaaaga gaagttgcag
     3541 gaactgcaca tctagtcagt aaactatctt cacaattaat tgaaaaaagc cagttcaaat
     3601 gaactggctt agttgtactt agtgcgcacc gcctccgccg ccacctgcgc caaatggcgg
     3661 tttagcaaac cacaccagcc ccagcagtac gaggaatatc ccggctgaca tccagaagat
     3721 ctcattggca gaaataatca gcccctgatt ggtgatctgc tgggcaatcc agcctgatgc
     3781 ctgctgttgc gtcatcccaa gcccttccag ttgactgtac atcgcctggg cattcgggtt
     3841 gaacgggttt accgactcag tcaactgcgc atggtgcatc gactcgcggt tggtccacat
     3901 ggtcgtggtt atcgacgtgc cgatagaccc cgccagcgtt cgcgtaaagt tagagaggct
     3961 cgatgccgcc gccagtcgtt ccggtggcaa accagacagc gtaatggtgg tcagcggcat
     4021 aaagaagcag gccaccgcaa acccctggat aaactgcggc caggccgacg cgccaaaatc
     4081 catacctggt tcaaaggtat aggcacgcca gtagaagcag acggcataca taataaagct
     4141 gaaggttacc agccgccgca tatccagttt atgcgcgaag cggccgataa tcggcgacag
     4201 gatcaccgga ataatcccta ccggcgcaga ggccaaacct gcccaggtcg ccgtgtaacc
     4261 gtagacctcc tgcaacaact gcggcagcag aacaatagcg ccgaagtaga gcatatacgc
     4321 gaggctgata cacaagcagc cgatggtgaa gttgcgcgac ttaaacaacg acagatcgac
     4381 tatcgggtta tcgtcggtca gctcccagac aatcaggaag cagatagcca ccaccgccac
     4441 cacggtaaga atgataattt cctgtgatga aaaccagtcc agctctttac cgcggtcgag
     4501 cataatctgc aggctgccga taccaataac cagcagtgcc agccccacgg catcaatccg
     4561 ccaccgttcg gtgcgggttt cacgtccgcg cagagtttgc agtgtcatca acaccaccgc
     4621 cacgccaatc ggcacgttga tgaagaatat ccagccccag tggtaattat cgctgatata
     4681 accgccgagg atcgggccgc aaattggcgc gacaatcacc gtcatcgacc acaacgccag
     4741 cgcgatcgag cgtttggctg gcgggtagtt attcagcaat agactttgcg aaagcgggat
     4801 caacggcccg gcgacaatcc cctgaatcac gcggaagaag atcagcatat tcaggctgct
     4861 ggagacacca cacgcccacg acgcaatagc aaaggcgatg gtggaccaaa ggaacagttt
     4921 cacttccccg acgcgctttg ccagccagcc ggtaagcggg atcgagatgg cattcgccac
     4981 cccgaaagaa gtgattaccc acgttccctg gctgagcgat gagcccagat tcccggcgat
     5041 agtggggatc gccacgttag caatggtgga gtccagcacc tgcatgaatg tcgccagtga
     5101 cagcgcaatc gtcataatga ccagttgcgc gccttccagc ggtttttgct gttgcatcac
     5161 acgcacctct ggattagcca gcgttagctt ttacgatatc gtcgatcagt ttattgacag
     5221 gtgccaggct gatttcacgc gcggtactta ccgcgaccgg tgttgaacgt actttatttg
     5281 ccagtacctg accgtcacgg ttagtggtat tgacgctcac cagcgtggac aaaccaatgc
     5341 gcagcgggta ttgctccagc tgtttctggt ccagttcgat acgcacaggt agacgctgaa
     5401 cgactttgat ccagttaccg gtcgcatttt gcgccggaag cagtgagaac gcgctacctg
     5461 tgcccatatc cagaccaacc actttaccgg tgtatttcac atcatcgccg taaatatcgg
     5521 tggtgatggt gaccggctga ccgatgcgca tattggcaat ctgcgtctct ttaaagttgg
     5581 catccaccca catattggtg gctggaacga ccgccatcag cggcgtcgtt gggctaattt
     5641 gcgcacccgg ttgcacggca cgacgggaaa cataaccagt catcggactg acaatacggg
     5701 tacgctccag cgccagccag gcgttacgta cttcggtggc agcctgttgc acggcaggct
     5761 gatcttccag tttagtcccc agaatcatcg cctgattggc attgtattgt tgaatagcga
     5821 cgtccagttg cgcctgggca ctggtgacgg cgtcgcgggc gtgttgcagc tcttcgcgac
     5881 caatcaggtt ggcattgccc agcggcacac ggcggttgta gtcgctttgt gctttcgcga
     5941 gggcgatttt ctgcacctca atattcgcct gcaactgctt gctgttaatc atcagctggt
     6001 gggtttggcg aacgctggaa gccagtgcag ttttggcttt ttcaaacgcc tggcgagcat
     6061 ctgtcgggtc gagagtgacc agaacatcgc cttcttttac aaaatcggtg ttatcggccc
     6121 agactttcgt cacgctgcca gacacctgag acataatttg aatctgattc cctgccacgt
     6181 atgcgtcatc ggtttcttcg aaatgacgca gtaccaaaaa ccaataaatc cctatcgcta
     6241 cggcaataat tataaagagc aaggtgagaa ggaggagcag acgcttacgt ttgccgctct
     6301 tctttaccgg ttgctgcggg gtttgagtct ccgcatttgc gctcatattg ttctccacga
     6361 tcttcttaac tcatcggctg agccgacctg cttgccaaaa aggccagcat gttgcgatgc
     6421 tggccagtca ttttttcttt tataaatctg gatttttgag cgagatgacg cgttagctca
     6481 tcgcttcgag aaccacaccg tcttgttcca tctggtcgag acgggagagc aatttgcggg
     6541 tgatttgctc gagctgatct ttttctgttg tgctgagcgc ggaccagagt tgatgcaggc
     6601 agttatgctg cggtggtaaa acctcgcgca aaaactcgtg acctttttcc gttaattgca
     6661 gatgcaggca gcggcgatcg ttatcgcttt cacgacgttc gatccaaccg cgtttttcca
     6721 gttcatcggc aatacgcgtc gcgttggtac gggatgatcc aagagcacaa cttaattcag
     6781 aaggctgaat actgtggttt tcctgagact ccagcgtaat caacgccata aacaacgtct
     6841 cgttaatccc ctgagccttc agcattttat tgcggttctc cagcagcttg ctttgcatgt
     6901 gcatgcaaag acgagtcaga aggatctcct gataaggaaa atcttcgtgg cggctggcgc
     6961 gaaattttag catttgttca atgggcgtaa acgaactatc catttgggta tgacctcatt
     7021 aatttcagcc gatatagtaa cgacagtgac aaataaagta aatgtattgt tttagaaaaa
     7081 tgattcttgt ggattatata atcgccatca ctttccagac gagcccgtag gtcagcgcac
     7141 taagcagtgt tgggatgata atgctgcgtg ttttatagaa actggcaccc agtaccgcga
     7201 agccgaccag cgtgggcacg aaacggcgtg tatcgtgcat cacttctggt gcggtagaga
     7261 caaccagcag agcgcatatc gaggcgatgc caatggtgtc gagcaaaata cctaccgcgc
     7321 cacgtttggt tgggcgggca ttacccacac gcaggcgcag cggcaaatag cggaagcaat
     7381 aattcgccgc gccaaccagt aacccaagca gcagaacctc atagctcatc gggcgctcct
     7441 tgccagaatg cctggattaa cgcagtgagg cagccacaga caatgcctgc cagaatggcg
     7501 acgggaatag aaaatagcgt tacgcctgca agggcaccaa ctaacgctgc ggtaacgcaa
     7561 agagattgtt tgcgctggaa agaggcgagc aggaaactca taaagagtgc cggaagcata
     7621 aaacctaatg ctgcttcaac ggcgggataa ccttgcagca agccgctgcc ggagaatgcc
     7681 cctattaccg taccaaatac ccacgatgac catgaactga aggcaatgcc gatcatccag
     7741 ttctcgctcc agcggcgatt attgcgtacc agttttgcgg ttgcggcggc aaaaacctca
     7801 tccgtcaggc caaacgccca cagggcggtt ttcgattttt gcagacgctg aataatacgg
     7861 ctacgcagtg acgggccata caacacatgg cgaacatcca ttgccatgac ggtcagtgca
     7921 gcaacccaca aactactccc ggctgccagc atcgcggtaa tgacgaactg gctcgcgcct
     7981 gcataaatga tgcaggagaa aaaaacgctt tcgagaggag agaatcccag acgggtcgca
     8041 ttcagaccga acgcaaaggc caccggaata taactaataa caatcggtaa actgtctttg
     8101 catccttcca tgaaggtcgc cgaaccagga gcaggctgtg gagtagggct ttccatagag
     8161 tgtacgctta acgattgtta atattataaa tagactgaat gaatatctta accttatcag
     8221 actgatgggc ttcttaacac ccttataagt gtaaagccac gaaaacggtt gctgattgct
     8281 taatttgcgg cttcctgacg atgaaaacct cgccaccaga ccagtaagtt aaccagggca
     8341 atcgtcgcgc cagccagaca aacgccagcc caaccgccat gttgccaggc tgaggctgaa
     8401 attagcgaac cggcggcacc gccaataaag tagctggtca tgtaacctgc ggtcaggcga
     8461 ttacgcgcat cagggtgtat tcgataaatt accgtctggt tagtgatatg cacgccctgt
     8521 acggtgagat ccagcaccag gatgccgata atcagcgcca gtacggaagt gtgaccaaac
     8581 cagatcgcca gccatgaaag taatagcagc agcagaccga aagttgtggt gtggtgcgat
     8641 ttacccttat cggcaaaacc gcccgccgga cgagcgccca acgctccggc agctcccgca
     8701 agtccaaaca gaccaatgac accatcgctg tagttaaaag gtggagcggc aagcaaaaag
     8761 gccattgagg tccagagaat gctgaagttg gcaaaggtca ggcagcccag caatgcgcgg
     8821 gtgcgcagga tcttatcgct gataaacatg ctgaaaacgg aacctaacaa ctgtgggtag
     8881 ttgaggtggg tttctgattt catttgtggc agaccacgcc ataatgccag cgccatcagt
     8941 gccattaaca ccgaggcaac ccaaaagacg gtgcgccagc cgccgagatt cgccagcaat
     9001 ccggcaacgg tccgtgcgag taagatcccc aacagcagac cgctcataat agtgccaacc
     9061 actttgccgc gtttgtccgg tgaagccagc gtcgctgcca gcggaaccag aatttgcgcc
     9121 acgactgaga ataaaccggt taatgccgta ccgaggatca tcatcgccag tgactgactg
     9181 ctggcagtga ttaacatacc gcccgctgcc agtaaggtca tcgagacaat caggcggcgg
     9241 cgttcaaaca tatcaccgag gggaacaaga aacagtagac ctgcggcata gcccaactgc
     9301 gcggcggtaa caataaagcc tgccgaactg gcggaaaggg aaaagttacg cgcgatggtg
     9361 tcgagcaatg gctgggcgta atagttactg gctaccgcca gaccggtggc gatagacatc
     9421 agcacgatca gcgccgggct aagctcatga ttaggtttag tcattatttt tgttcatcag
     9481 gtgatgatta agctggaaat caataatagc ggatgttgat gaatatggga tgaaagatag
     9541 attgttgaaa tgtgcgatgt ggtttgtagg catgataaga cgcgccagcg tcgcatcagg
     9601 cattgtgcac agaacggcgg atgcggcgaa aacgccttat ccgccatacg aatgagtgta
     9661 ttttgtaggc atgataagac gcgtcagcgt cgcatcaggc attgtgtaca gagcggcgga
     9721 tgaggcaaaa acgccttatc cgcctgaata aaaattactt ctgcgctgcc agcgcctcat
     9781 tcacccagcc atcgaactgc tgctggtggg ctttgatcca gccatcaacg tggccctgaa
     9841 tatcgccttc tgaggctttg ccgtcatgca taatggcgtt ctgggcgtta atatcggcca
     9901 ctggcaactg cataatggca aacagtttgg ctgccgccgg atttttctcg gtccaggctt
     9961 tgttggcaac gatatgcatg gtgctgaccg ggaagccgta attcgcgcca ttcggcagtt
    10021 tggtatcggc atttttatcg cccggcagtg cggagaacgg cacctgcaac cagaccacat
    10081 ctttccctgg cttcagctca ttactcaccc agtacggcgt ccaggtgtaa taaaacaccg
    10141 gtttgccctc tttgtagcga ctgatggtgt cggccatcat cgctgcgtag ttcccctgat
    10201 tatgcgtcac ggtgttggtc agttcatacg cggcaagctg gtggttgatc gcaccttcgc
    10261 agccccagcc agggttacaa ccggttaaat ccgctttacc gtcgccgttg gtatcgaaca
    10321 gtttggcgat cttcggatct ttcagttgtg cgatgttggt gattttatac tggtcggcgg
    10381 ttttcttatc gatcaggtaa ccctgtgccg cgccgttaac aaatacccct tcacgataaa
    10441 atttcttatc gccaccggca gcttcgtaca tgttgtcatg cagtggcgtc cagttcacgg
    10501 cggtgaaggt tgcatcgccg gaagcaagcg aggtgtagcc aacgttgtaa tctacttcgc
    10561 tgggtttgtt gacggtatag cctaatttct ccagcgcacg gctgaccagc agcgtctgga
    10621 aggtttcttc ggtgatggtg ctttgaactg gatttacagt aatacctttg cccggcagat
    10681 cggcagcaaa agtttgtgta gagataagcg tggcaaacgc tgtcgcaaaa agtacgctat
    10741 gtcgcattgt tattcctttt tttggcaggg tgataatgcc cggcgtcacc gggcaagtgc
    10801 agagttactt aatgaatggg cgggtcagca gaccaacagg gccagtggtg taccagcgac
    10861 ggttgccgcg actgcgtgag tcgcgcccaa cggcctgcgt caggcgatcg aggataatgg
    10921 cgaggatcac aatcccgacg ccgccaacgg tggcaagccc catatccaga cgaccgatac
    10981 cgcgaagtac catctgaccc agcccgccga cggcaatcat cgaggcaatg accaccatag
    11041 aaagggccag catcagcgtc tggttaacgc ccgccataat ggtcggcatc gccagcggta
    11101 actgaacttt gaacagcatc tggcgcgggc tggcaccgaa tgagcgcgag gcttcaatca
    11161 gatccgccgg aacctggtta atccccagaa tggtcagacg gataatcggc ggcagagcaa
    11221 agatgatcgt caccaccacg cccggcacgt taccgatacc aaatagcatg acgattggca
    11281 ccagataaac aaacgctggc gtggtctgca tggcatcaag cagtggacga ataattttcg
    11341 ccgctcgcgg acttctcgcc agccagatcc ccagcggcaa accaatgacg atacagaaca
    11401 gcagggcggt taacaccagc gccagggtaa ccatggcctg cgaccaggca ccgattgcgc
    11461 cgatggcaat cagcgaaacc agcgtcgcca tgcccattcc gaccccggaa atctgccagg
    11521 cgatgagagc gaaaacgata atcgccaccg gtgcgggcat acccagcagc aattgctgga
    11581 aaccgttgag aatgtaatca accggaacgc gcacgccctg gaagacggga cggaaatggg
    11641 taacaaccca gtcgatccct tcagtgaccc aactgtcgag cgggatcagc gttttatgga
    11701 acggatcgag aatattaaaa tgctcgacgt ttggcgcagg cgtactggtc agccagtcag
    11761 caccaccgcc gtcagtcggt gcagtcgccg gtgtacccca ggcgtctgcg gattgcacgg
    11821 cactgtccgc cgctggcgtg gtatcccacg gattattttg atcagccatt atttaccccc
    11881 tcacgatcta aagcgcgcag cagcattcct ttcgaaatga tgccgacata ctgttggtcc
    11941 tcgtcgacca cgggcaccgc acagggtgcc tgtccgacat gagagagcaa ctcgctaaga
    12001 ggcgtttgtg catcgactgc taacggcgca tcaatcagcg ccgcatcaag accttgctgc
    12061 tgcgttaacg cggttttaag cgaatcgatg gaaactgcgc cgacaaactt attaccgcgt
    12121 tcgataacgt agccatattc gcgatcttca tcctgcaata atttcagtgc cgaacgtggg
    12181 ccgaagccag gggttttacg aattaagcca ttcggtgtcc ggcgggcaat atctttcgca
    12241 ctgaatacct gactaatatc aacgccacgg aagaaggtac ggacataatc attcgccgga
    12301 ttattgagaa tttcatccgg tgtgccgacc tgtaccactt caccattttg cataatggca
    12361 attcggtcgc caatacgcat agcttcatcg agatcgtggg aaataaagac aatggtgcgc
    12421 tgatgtttcg cctgtaattt taccagctca tcctgcatct cggtgcgaat taatggatcg
    12481 agcgccgaga aggcttcgtc cattaataat atatccggat taatcgctaa cgcgcgggct
    12541 aatcccacac gttgacgcat cccgccagag agttcatccg ggtagctgtg ggcataattt
    12601 tccagcccga cctgacgcag tgcatcaagg gctttttccc ggcgttcttc ggcattaatt
    12661 ccggccaatt ccataccgaa cgcagtattg tccagcacgg tcatatgcgg cattaaggca
    12721 aaggactgga agaccatcgc aatctttttt ctgcgcacct cacggagttc ggcatcggat
    12781 attttggcaa tatccacacc atcaatcagc acttgcccgc gggtgggttc aatcaggcga
    12841 ttgagaaggc gtaccattgt ggatttaccc gagccggata atcccatgat gacaaatatc
    12901 tcgccttctt caatggccag actggcgtct tttacgccaa gcgatagccc agttttttcc
    12961 agaatttgtt ctttcgaaag tccttgttcg atatatttga acgctcgctg tggatgctcg
    13021 ccaaatattt tataaagatt tttaatttct aatttaattg ccatgcaata gaaagattcc
    13081 tttatttgtt tatgtcgata tattacccaa tggaaatagt cacttttctc taccctaaca
    13141 tactgagaat ctgaggcaac ccctgagggc aaatgtggca tgaatattga tgcgagcaaa
    13201 tcgccggaat ttcccgtgat ataagggctg agagcaaatc gagaaaagtt gatatttttt
    13261 gtcatagata gcgaaaaacg cctgaatatt gtagtttcag gcgaataatg caacagagta
    13321 tcagtgtaga tcactacaga atatttgcgt gataaaaagc tatttggcgg gaattatttc
    13381 cctgctgcgg gtagtgatat ttttgaaaat aacaccctca gaaattccag tcttcatctt
    13441 ctgtttcaac cgctttcccc atcacataag aggagcctga accggaaaag aaatcgtgat
    13501 tttcatcggc attcggcgaa agcgcggcga ggattgccgg attcacttcc gccatttctg
    13561 cgggaaataa cggttcgtag cccagattca tcaaagcctt attggcgttg taacagagaa
    13621 acgctttcac atcgtcagcc cacggggttt cggcgtacag ctcatcggtg tattgcaact
    13681 cgttgtcgta gagttccagc aacaaatcga aggcgaaact cttcaactct tcacgttgtc
    13741 ccagagatat cttttccatg tttttctgat atttatagcc tatgtagtaa ccgtggactg
    13801 cttcatcgcg gataatcaga cggatcagat ctgcggtatt ggtcagcttt ccgcggctgg
    13861 aaaaatacat cggcagccag aaaccggaat agaacaaaaa agattcaaga aacacactgg
    13921 cgattttctt tttcagcgga tcatcaccgc gataatgttg ctgaataatc tgagcttttc
    13981 gctgcaacgg tgcgttttct tcactccagg cgtaggcggc atcgacatct ttggtctgac
    14041 acagcgtcga gaaaatcgaa ctgtaagagc gggcatgaac cgcttccata aagctgatat
    14101 tcgataatac cgcttcttca tgaggcgtga gtgcatcggg catcagcgaa ggcgcgccga
    14161 taacattttg cagcgtgtcg agcagcgtca ggccagtaaa aacgcgcatc gtcagttgtt
    14221 gttctacgac agttaatgtc tgccaggcag gaatatcgtt cgacagcggc accttttctg
    14281 gtagccagaa attgctggtc aggcgattcc acacctccag atctttatcg tcagatatct
    14341 tgttccagtt gatggtgctg atacgtgaga gtttcataga tattccttaa agtgcacagg
    14401 agacgcagcc ttcaatttca gtgccttcca gcgccatctg acgcaggcga atgtaataga
    14461 gcgttttgat acctttgcgc caggcgtaaa tctgcgcttt gttgatatcg cgagtggtgg
    14521 cggtatcggg gaaaaacagc gtcagcgaca aaccctgatc gacatggcga gtcgcttccg
    14581 cgtaggtgtc gatgatcttt tctgcgccga tttcgtaagc atcctgatac agcgccagat
    14641 tctcgttagt cataaacggg gcagggtagt aaacgcgtcc tgttttgccc tctttgcgta
    14701 tctctacttt cgccacaatc ggatgaatac tcgacgtagc atggttgata taagagatag
    14761 aaccggttgg cggcaccgcc tgaagattct ggttataaat gccgtagcgc atcacgtcgt
    14821 cgcgcagttg cgcccacatc tcacgggtag gtaacgtaat accgctgcgg gcaaacagtt
    14881 cgccaacttt cgccgttttc ggctgccagt taccttgcag atattggctg aaatattcac
    14941 cactggcata gcgtgactgt ttgaacccgg cgaaggtttc accgcgttcg cgcgccagca
    15001 acatcgaggt acgcagtgcg tgccaggtga tggtatagaa atagagattg gtgaaatcca
    15061 gggcttccgg cgaaccataa gcgatgcctt ccctcgccag ataaccatgt aaattcatct
    15121 gccccagtcc gatggcgtgc gaggcggcat ttccggcttc gatggacggc acgctgcgga
    15181 tatgactcat atctgacacc gctgttaaac cacgtacggc agtctctacc gtgcgggcaa
    15241 agtcggggga atccatggtg tgagcaatat tcaacgaacc taaattgcag gagatatcat
    15301 ggcctgtgcg ggcatagtcg agattctcgt catactctga ggcacagttg acctgcaaaa
    15361 tctctgagca aagattgctc atatttatgc gcccggcgat agggttagca cggtttactg
    15421 tgtcttcata catgatgtag ggatagccgg actcaaactg gatttctgcc agtcgctgga
    15481 agaaatcacg ggcgttgagg tattttttgc ggatgcgttc atcggcaacc agttcgtcat
    15541 agtgttcgct aatggcgata tcggcaaacg gcttgccata aactcgctca acgtcatagg
    15601 gcgaaaacag cgccatctgc gcattctctt ttgccagatg gaaagtgata tccgggatca
    15661 ccacgccaag cgacagtgtt ttaatgcgga ttttttcgtc ggcattttcc cgtttcgtgt
    15721 cgagaaaacg cagaatatcg ggatgatgag catgtaaata gactgcacct gccccctgac
    15781 gagcgccgag ttggttggca taggaaaatg cgtcttccaa cattttcatc accggaatta
    15841 cgccagaaga ttgattttca atacgtttaa ttggcgcgcc cgcttcccgc agattcgaca
    15901 gcaaaaatgc tacgccgccg ccgcgtttcg acagctgcag tgcggaattt accgcccgac
    15961 caatcgactc catattgtct tcaatacgca gcaaaaaaca ggaaaccagt tcgccgcgct
    16021 gctgcttacc gcagttgagg aatgttggcg tggctggctg aaagcgtcct gacagcattt
    16081 catcggtcag ttgcaacgcc agcgtctcat cgccttgtgc cagcgtcagc gccaccatcg
    16141 ttactcgatc ggcaaaatct tccagataac gtttaccgtc gaatgtcttc aacgtatagc
    16201 tggtgtaaaa cttccatgcc ccgaggaatg tctggaaacg aaaaccgctg gtgtgtgcgt
    16261 gggcaaacag cgtaatgaca aagtcgcgag agtagcgatt aagaacgctt tcatcatagt
    16321 aaccttcgtt gaccagccag ttcaggcgct gctgctggct actgaaggtc acactgttcg
    16381 gacgcacatg cgtcgcaata aaggcgtcaa cggcctggcg gtctttatcg aactgaatgc
    16441 gacctgcgct atcgtagagg ttaagcatcg cattcagcgc gtggtaatcc atcgtttcct
    16501 gcgtcaggca ttctgcggtt gtcgttgcca aaattcggtt actcctttac gaacgttttc
    16561 gatatcgctt tgcgtaccca tgagttcaaa acggtacagc cacggcacgc cgcatttccg
    16621 ggcaatcaca tctccggcgc ggccatacgc ctcaccaaag ttgcgattac cagaagcaat
    16681 aacgccgcga agcaacgccc ggttgtgctc gtcgtttaaa aagcgaatta cctgtcgtgg
    16741 caccgcgcca gccgtaccgc cgccgccgta agagggcacg atcaggatgt aaggctcgtc
    16801 tacctgaatc cgttcccgct cattgagcgg gatgcgcacc gcgggcagac ctaaacgttc
    16861 gataaaacgc tgcgtgtttt cggagctgct ggagaagtag acgagctggc tcatgcactg
    16921 gccgcgtgtg gcgctggatg cagacggtta atcatgtccg gacggaaacc agaccagcta
    16981 agatcgccag caatcactac cggcaactga cgaaagccct gagcacgcaa cgcttctgcc
    17041 gcttcaggaa cgcgatcgac attaatcatt tcaaaatcaa agccccgatt ttccatcgcc
    17101 cgtttggtgg cgtggcactg aacgcaatcg ttacgagtgt aaatagtaat gcgcatgatt
    17161 cgtatttccg tttaaaatga agatacggcg cgatgatacg cgtcgggttg tctttctgtt
    17221 gatacagaga tactagatgt agttgaaaaa agattcaacc acacaatata tagcaaatag
    17281 tggtcgaaat taccctggat atgagcgtga cggggtaggg ggatttttgt gattcaccag
    17341 gcaaaaagaa accccgaaga caggcttcgg ggttaaagac gcgtatttat tatcattttt
    17401 gcactacgat ttgcgcatgc ttaacagtgc gccgataaaa atacctaccg cagctgctgt
    17461 acccacgcta caccagggtc tttcacgaac aaaagaatcc gcgcagccaa cggcatcgcg
    17521 tgcggcttgc tggacgcgag tacgaccatg cattcgcgcc ctggtttctt tcagcaatgc
    17581 ctgagcttta ctgcgtgcgg cttcagcttc ccctttggcg tcgctgcccc aggacttcaa
    17641 tacagattcg aggctgtcag ctaattgatt gacatcattc tggatatcct gcacgccatc
    17701 atcaacgtcg ttgcggttcg gtcggttaaa catatgctcc cccattagaa ttcgatgtaa
    17761 ttttcagtgt agaccataat tttcttaact gaggttcatc gcggctttat ggacttttct
    17821 gaaagcgttg tgaatgcgca atcaggtaag gtaggcccgc ggttgataat aacgaggatc
    17881 aaatatgtat ttacgaccag acgaggtggc gcgcgtactt gaaaaagtcg gttttactgt
    17941 cgatgtggta acccagaaag cgtatggtta ccgccgtggg gaaaattatg tctatgttaa
    18001 tcgcgaagcg cgaatgggac gtaccgctct ggtaattcat ccaacattaa aagaacgcag
    18061 ttcgacactg gctgaacccg cttccgatat taaaacttgc gatcattacc agcaatttcc
    18121 gctctattta gcaggcgagc gacacgagca ttacggtatc ccgcatggct ttagttcgcg
    18181 tgttgcgctt gaacgttatt tgaatggttt atttggcgaa gccagttaaa gacgcgactg
    18241 gcgatgccag tcgcgaaaag aagagtcagg cttttacctg ctggtaacgg ctgactttaa
    18301 acagtcggcg gcaataatcg aggaagtagc cataaaccgc ccccatcaac atcgaaacaa
    18361 cgatgtttga actgaccgcc gccataatct gatgccagtc tgcgcccacc actaacaaga
    18421 tcgccacata caccggtgac tggaacgtca cataagccag gatatccgcc agatttttta
    18481 tccagcccga cgggctaact ttgcgtgccg cgcgcataaa cagatcacgg tacataccgt
    18541 atggccatgc aattaagatg ttcaccggaa tcgctaccaa tctggaataa aaagactgtt
    18601 cgaagctcat tccggagagg aaaacttcaa tacacatgtt cacgacagaa cagtaaacaa
    18661 ccatcgcgaa cgtatctgca actgcatgac gcaagcgtga ctgcggtgag aacatgctaa
    18721 tgctccttaa taaaagcgta atgggatgtt aatggagatg aaaacagtgg atctcattgc
    18781 catctgcatt ttcgcatagc gtatatcgct ggatatggct aatcaattag tgaaaaattt
    18841 ttatttagtc agtttttgta gataaaatat tccgtaacgg ctcgtttttt gtacattgtg
    18901 gatttttaca tgccgatatg at
//
LOCUS       DAZGSP010000097        18500 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZGSP010000097 DAZGSP010000000
VERSION     DAZGSP010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.098"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            293..877
                     /gene="yadN"
                     /locus_tag="ACHK74_003813"
     CDS             293..877
                     /gene="yadN"
                     /locus_tag="ACHK74_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0222554.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            982..1722
                     /gene="yadV"
                     /locus_tag="ACHK74_003814"
     CDS             982..1722
                     /gene="yadV"
                     /locus_tag="ACHK74_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFP0222555.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1757..4357
                     /gene="htrE"
                     /locus_tag="ACHK74_003815"
     CDS             1757..4357
                     /gene="htrE"
                     /locus_tag="ACHK74_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFP0222556.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGHCLNLTEIIPQASVRYDVNDQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAVMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYVQRDIASLRSQLIIGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQILKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQLTDNNYTAGLLGLGLNTSVGAFSFDVTHSNVSIPDDKTYEGQSYRVSWN
                     KLFENTKTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKNMRNYSRMKNQVT
                     VSINQPLKLEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSISAQRSWN
                     EDGDTDDSVYLSFTVPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNISSSGYSDNA
                     RVSYSVNTGYTMNKASEDLSYVGGYASYESPWGTLAGSVSANSDNSSQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFAGFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIDQQGNISIKWIEESKPVSCLAHYQQSSEAEKLAQSI
                     ILNGIRCQIQ"
     gene            4374..4949
                     /gene="yadM"
                     /locus_tag="ACHK74_003816"
     CDS             4374..4949
                     /gene="yadM"
                     /locus_tag="ACHK74_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0222557.1"
                     /translation="MIKTTPHKIAILMGILLSPSVFATTDINVEFTATVKATTCNITL
                     TGTNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVARECSTGISWIDTTLTGNS
                     SSSSPKLLIPQSGDSSSTTSYICMGFKKRTTDDATFLQPNNSAEKIRWSTNEMQPDKG
                     LEMTVALRETEAGKGVPGNFRALATFNFIYQ"
     gene            4964..5566
                     /gene="yadL"
                     /locus_tag="ACHK74_003817"
     CDS             4964..5566
                     /gene="yadL"
                     /locus_tag="ACHK74_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFP0222558.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEMGT
                     CTATLVNDSDQNISVVDFDDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTVTPATGSATQFSCVTPASQEVTISTA
                     ANEVVYYPMSARLVVEKNKTVNNVTAGKFSAPATFTVTYN"
     gene            5593..6189
                     /locus_tag="ACHK74_003818"
     CDS             5593..6189
                     /locus_tag="ACHK74_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFP0222559.1"
                     /translation="MHPTQRKLMKRIILFLSLLFPITCPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSGNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPANIFNVISSAGNSRSVYPVMTS
                     ALNNSSWKFYARMQKIDPALDVISGQVISHILVDVYYE"
     gene            6203..7516
                     /locus_tag="ACHK74_003819"
     CDS             6203..7516
                     /locus_tag="ACHK74_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFP0222560.1"
                     /translation="MTATVTAVDGGVIMKKFFRHFLFLILCLNCYTASAGTDDNVGYI
                     VGNSYGVGPSDQKWRETGPNGDATVVFRYATSTNNLVFYKPTQLGPTGVKLQWSQLDT
                     ASGGGFLYCNRSDSTSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYYTLLISNMWSA
                     YGTVTNVSSPGIYIGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAVGGIYQTLTIE
                     FYTDTNFDPTVTQQIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFDLLNVKLTNPT
                     CFTAMLSGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIETKLVSTKVG
                     TENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYDPRGKDDTT
                     GGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            7629..8423
                     /gene="panB"
                     /locus_tag="ACHK74_003820"
     CDS             7629..8423
                     /gene="panB"
                     /locus_tag="ACHK74_003820"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFP0222561.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8435..9286
                     /gene="panC"
                     /locus_tag="ACHK74_003821"
     CDS             8435..9286
                     /gene="panC"
                     /locus_tag="ACHK74_003821"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFP0222562.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(9360..10262)
                     /gene="rpnC"
                     /locus_tag="ACHK74_003822"
     CDS             complement(9360..10262)
                     /gene="rpnC"
                     /locus_tag="ACHK74_003822"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFP0222563.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMTNLSLAEIDKVINLI"
     gene            10536..10916
                     /gene="panD"
                     /locus_tag="ACHK74_003823"
     CDS             10536..10916
                     /gene="panD"
                     /locus_tag="ACHK74_003823"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFP0222564.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10920..12149)
                     /gene="yadE"
                     /locus_tag="ACHK74_003824"
     CDS             complement(10920..12149)
                     /gene="yadE"
                     /locus_tag="ACHK74_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP0222565.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12213..12653)
                     /gene="yadI"
                     /locus_tag="ACHK74_003825"
     CDS             complement(12213..12653)
                     /gene="yadI"
                     /locus_tag="ACHK74_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP0222566.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12758..13528)
                     /gene="yadH"
                     /locus_tag="ACHK74_003826"
     CDS             complement(12758..13528)
                     /gene="yadH"
                     /locus_tag="ACHK74_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0222567.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13525..14451)
                     /gene="yadG"
                     /locus_tag="ACHK74_003827"
     CDS             complement(13525..14451)
                     /gene="yadG"
                     /locus_tag="ACHK74_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0222568.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14560..15222
                     /gene="can"
                     /locus_tag="ACHK74_003828"
     CDS             14560..15222
                     /gene="can"
                     /locus_tag="ACHK74_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFP0222569.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15263..15799)
                     /gene="hpt"
                     /locus_tag="ACHK74_003829"
     CDS             complement(15263..15799)
                     /gene="hpt"
                     /locus_tag="ACHK74_003829"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFP0222570.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYICKVILLDE"
     gene            16005..18395
                     /gene="gcd"
                     /locus_tag="ACHK74_003830"
     CDS             16005..18395
                     /gene="gcd"
                     /locus_tag="ACHK74_003830"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFP0222571.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSANATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
BASE COUNT         4997 a         4445 c         4323 g         4735 t
ORIGIN      
        1 catttttgca gaaacataat ataaattccc gcagataaat atcaatccca tcgccatacc
       61 ctaaccaatt gcacttgtga atttattttt gcttaatttt gaattaagaa aatttaattt
      121 gttcaaaagc ccttaaagtg cacaataggc gtcctaatga acaatggttg taaattttgg
      181 taacaaatat cattgctcat taatatctag catctgctta taacatttgt gtcatgaata
      241 gtcattcttc aaaccagtag tctgactaat ggaattttat tcaaggattt ttatgtctaa
      301 aaaattaggt tttgccctgt ctggtcttat gctggcaatg gttgcaggta ctgcttctgc
      361 tgatatggat ggtggtcagt taaatatcag tggtctggtt gttgataaca cctgtgaaac
      421 ccgcgttgac ggcggtaata aagatggtct gatcctgctg caaactgcaa ctgttggtga
      481 aatcgatgct ggcgtgctga atgacaccgt tggcgctaaa gccaaacctt tcagcatcac
      541 cgttgactgc agcaaagcaa atccgaatcc aggtagtaca gctaaaatga ccttcggttc
      601 tgttttcttt ggtaatagca aaggaacgct gaacaacgac atgtctatta acaacccgtc
      661 tgacggcgtg aatatcgctc tgcacaatat tgatggttcc actatcaaac aggttcaaat
      721 taacaaccct ggcgatgtgt atactaaagc cctggatgca acgacaaaat ctgctgttta
      781 tgattttaaa gcgtcttacg ttcgtgccgt cgcagaccaa acagcaactg ctggttatgt
      841 aaaaactaac actgcataca ccattactta tcagtaagta ttcaccaatt aaattgattg
      901 aatgaatata cagggaatag tgttaataat ttctattttt atattattcc ctgttttaat
      961 taactctgtc agggatggtt tatgtttttt aacaccaaac atacaacagc tttatgcttc
     1021 gtaacctgta tggcttttag ttcatcgtct attgcggaca ttgtcatttc gggtactcgc
     1081 gtaatatata aaagcgatca aaaaagtgtc aacgtacgtc tggaaaataa agggaataac
     1141 ccgttgcttg tccagagttg gttagatact ggcgatgaca acgctgagcc tggcagtatt
     1201 acagtccctt ttactgccac gccgccagta tcgcgtattg atgccaaacg tgggcaaaca
     1261 atcaaattaa tgtacacagc cagcacctca ctgcctaaag acagagagag cgtgttctgg
     1321 tttaacgtac tggaagttcc accaaaacca gatgcagaaa aggtcgcgaa tcaaagtctg
     1381 ctgcaactgg catttcgcac acgtataaaa cttttctatc gcccggatgg attgaaggga
     1441 aatccctctg aagccccgtt agccctgaag tggttctggt caggttcaga aggtaaggcg
     1501 tcattgcgag tgaccaatcc aaccccttac tacgtctctt ttagcagtgg tgatttagaa
     1561 gctagcggta aacgctatcc gattgatgtg aaaatgattg caccatttag tgatgaggtc
     1621 atgaaagtca ctggccttaa tggcaaagca agttctgcaa aagtacattt ttacgccatt
     1681 aatgattttg gtggcgccat tgaaggtaat gccagtctgt aatcaggcaa ggatataatt
     1741 ccgcaggaag cataacgtga ctataaaacc aactaaaaat tatcataatc atctgacccg
     1801 tatcgctacg ttttgcgcgc tgctgtattg caatagtgct ttctgtgctg aacttgttga
     1861 atatgaccat accttcctga tggggcagaa tgcctcaaat attgatctca gccggtatag
     1921 tgaaggtaac cctgctatac cgggtatgta cgacgtcagt gtttatgtca acgatcaacc
     1981 tattattaac caaagcatta catttattga aattgaagga aaaaaaaacg cacaagcctg
     2041 tatcacactt aagaatttat tgcagtttca tataaattct cccgatataa ataacgaaaa
     2101 agccgttctg cttgccaggg atgaaacact cggccattgc cttaatttga cggaaattat
     2161 ccctcaggct tctgttcgtt atgacgttaa cgatcaacgt ctggatatag acgttcctca
     2221 ggcctgggta atgaaaaatt accaaaacta tgttgatcca tcgttatggg aaaatggcat
     2281 taatgcggtc atgttgtcat acaacctcaa cggatatcat agtgaaactc ctggtcgaag
     2341 aaatgacagc atttatgctg cttttaacgg tgggatgaat ttaggtgcat ggcgactgcg
     2401 tgcctcaggc aactacaact ggatgaccga ttctggcagt aattatgatt ttaagaatcg
     2461 gtatgtccag cgtgatatcg cctcgctgcg ttctcaactc attattggtg agtcttatac
     2521 aacgggcgaa acctttgatt ctgtcagtat ccgcggcatt cgcttataca gtgacagccg
     2581 catgttacct ccgacattag ccagttttgc gcctatcatt catggtgttg ccaataccaa
     2641 cgccaaagtc actattacgc aaggtggcta taagatttat gaaacgactg tgccgccagg
     2701 cgctttcgtc attgatgatc tgagtccgtc aggttacggc agcgatctta ttgttaccgt
     2761 cgaagagtcg gatggctcaa agcggacatt ctcgcaacct ttttcatcgg ttgttcaaat
     2821 gttacgccca ggcgttggac gttgggatat tagcggcggt cagattttaa aagatgatat
     2881 tcaggatgag cctaatttat ttcaggccag ctactactat ggtttgaata actacctgac
     2941 gggttatacc ggtattcagc ttaccgataa taattatacc gccgggttgt taggtcttgg
     3001 tctgaatacc tcagtcggtg cgttttcttt cgatgtaact cactccaatg ttagtatccc
     3061 ggatgataaa acgtacgagg gtcaaagtta tcgcgtttcc tggaacaagt tatttgaaaa
     3121 taccaaaacc tcgctcaata tcgcggctta tcgttattca acgcagaatt atctggggct
     3181 taacgatgcc ctgacactga tcgacgaagt aaaacatccg gaacaggacc tggaacctaa
     3241 aaatatgcgg aactattcac gcatgaaaaa tcaggttacg gtcagtatta accaaccgtt
     3301 gaaattagag aaaaaagatt acggttcttt ttatctttct ggtagttggt ctgattactg
     3361 ggcttccgga caaaatcgta gcaattactc cattggctat agtaacagtg catcctgggg
     3421 cagttacagc atcagtgcac agcgttcatg gaatgaagac ggcgacactg acgatagcgt
     3481 ttatctcagt ttcaccgttc ctattgaaaa attacttggc actgaacaac gtacttcagg
     3541 ttttcagagt attgatactc aaatgagcag tgactttaag gggaataacc aactcaacat
     3601 cagtagcagt ggctacagcg ataacgctcg tgtcagttat agcgtaaata ctggctatac
     3661 gatgaataaa gccagcgaag atttgagtta tgttgggggt tatgccagct atgaatcacc
     3721 gtggggaacc ctggcaggtt cagtgtctgc aaatagcgat aacagcagtc aagtttctct
     3781 cagcaccgac ggtggttttg tattacatag cggcggcctg accttcagca atgacagttt
     3841 tagcgactcc gatacactgg cggtagttca ggctccaggt gctcaaggag cgcgaataaa
     3901 ttatggcaac agtactatcg atcgatgggg ttatggtgtt accagtgctc tatctcctta
     3961 tcatgaaaac cgcattgcgc tggatattaa cgatcttgag aacgatgttg aattaaaaag
     4021 taccagtgct gtagctgtgc cgcgtcaggg ttcagtcgtc tttgctggtt ttgaaaccgt
     4081 gcaagggcaa tcagccatta tgaacatcac acgaagtgat ggtaaaaata ttccatttgc
     4141 tgcagatatt tatgatgagc aaggcaatgt cattggtaat gttggacagg gtggacaggc
     4201 ttttgttcgt ggtattgatc agcagggaaa tatcagcatt aaatggatag aagaaagcaa
     4261 acccgtaagt tgccttgcgc attatcaaca aagttcagag gcagaaaaat tagcccaatc
     4321 tattattctg aatggaattc ggtgtcagat tcagtaacta caagggacgt caaatgataa
     4381 aaacaacgcc acataaaata gcgatactga tgggaatatt attatcaccc tcagtatttg
     4441 caacgacgga tattaatgtt gagttcacgg ccaccgtcaa agcgacaacc tgtaacatca
     4501 cccttactgg tactaacgtc acgaatgatg gcaataataa ctacacattg agaatcccta
     4561 agatggggct ggataagatc gcgaataaaa cgacagaatc tcaggctgat tttaaactgg
     4621 ttgccagaga gtgcagcact ggcatcagtt ggatagatac cactctgacc ggaaactcat
     4681 catcaagctc acctaagctt cttataccgc agtctggtga ttcatcatcg acgacaagtt
     4741 atatctgtat gggtttcaaa aaacggacta ctgatgatgc aactttcctt caacctaaca
     4801 acagtgcgga aaagatccgc tggagcacaa acgaaatgca gcccgataaa ggtcttgaaa
     4861 tgaccgttgc gctacgtgaa acagaggctg ggaaaggtgt acctggaaat tttcgtgcgc
     4921 tagcgacgtt taatttcatc tatcaataaa aaggataatt aagatgacat ttaaaaattt
     4981 acgttatgga ttatccagca gtgtcgtttt ggctgcctca ttgttcagcg taccctctta
     5041 tgccgctact gacagcatcg ggctgaccgt tattaccacc gtagaaatgg gaacttgtac
     5101 ggctacattg gtaaatgact ctgatcagaa catttctgtt gttgattttg atgatgtata
     5161 tatttctgaa atcaatgcca agaccaaagt aaaaacattc aaactcaaat tcaaagactg
     5221 tgcgggtatc cccaataaaa aagcgcaaat aaaattaacc aagcgagcca catgcgaagg
     5281 aacggctaat gacggtgcgg ggtttgcaaa tggttccaca gccgcagata aagcaagtgc
     5341 cgtcgccgtt gaagtctgga gcactgtaac tccggcaaca ggaagtgcaa cacaatttag
     5401 ttgtgtgaca ccagcatctc aagaggtaac aatctccact gcagccaacg aggtggttta
     5461 ttatccgatg agtgcacgcc tggtcgtcga aaaaaataaa accgtaaaca atgtcacggc
     5521 gggtaaattt tctgcaccag ccacatttac agtaacctat aactaaaaga cttacagatt
     5581 caggaactat atatgcatcc cactcaacgt aagctgatga agagaataat tctgtttctg
     5641 tcactactgt ttcccattac ctgtccagcc atcgctgggc aggatattga ccttgttgcc
     5701 aatgtaaaaa acagcacttg caaaagcgga atcagtaacc agggtaatat tgatcttggc
     5761 gtcgttgggg tgggatattt ttccggtaat gttactcctg aaagctatca accaggtgga
     5821 aaagagttca ctgtcacagt atcagactgt gcattacagg gaactggcga tgtgctaaat
     5881 cagttacata ttgattttag agcccttagc ggtgtcatgg ctgcaggttc aaggcaaata
     5941 tttgccaatg aagtcgcaac cggggctaaa aatgtcggtg tcgttctctt ttccattcag
     6001 gacccggcca atatattcaa tgttattagt tcagcgggaa attctcgttc cgtgtatcca
     6061 gtaatgactt ctgccctgaa taattcttca tggaaatttt atgccagaat gcaaaaaatc
     6121 gatcccgcgc tggatgtcat atctggccag gtcataagtc atatattagt tgatgtgtac
     6181 tacgaataat agatcaaaac aaatgactgc gactgttaca gcagtagatg gtggagtgat
     6241 aatgaagaaa ttcttcagac attttctttt tttaattctg tgcttgaatt gttataccgc
     6301 gagtgctggc actgatgata acgtaggata tattgttggg aatagttacg gcgtaggtcc
     6361 gtcagatcaa aaatggagag aaacggggcc gaatggcgat gctactgtcg tattcagata
     6421 tgcaacaagt acaaataatt tagttttcta caaaccgacg cagcttggac ctacaggtgt
     6481 aaaattacag tggagtcagt tagataccgc ttctggtggt ggttttcttt attgcaacag
     6541 aagtgatagc acaagtggta gcgcaatgcg tattgaaaat gcaatggttg actcaggtaa
     6601 aatgtatggc tcccataaat tatttaatac atcagttcct ggtttgtatt acacattatt
     6661 aatttcaaac atgtggtcag cttacggtac cgtaactaac gttagttctc ctgggatata
     6721 cattggtgac tctgcagaac aatatttttc gtggtataat ccaagcgaag atgtgttata
     6781 ttggagttgc aataatgcga atagcacccg taaatactgg gctgtaggtg gtatttatca
     6841 gacccttaca attgaattct atacagatac aaactttgat ccaacagtaa ctcaacagat
     6901 caaattatcc agctcatcaa attatctgta ttcatttaaa gcctatggcg caggccaagg
     6961 tataaatgag catagttatt ttatcaaaat cgatttcgat ctgctcaatg tcaaattgac
     7021 taatcccact tgttttaccg ctatgcttag tggaacttct gtgactggtt ctacggtaaa
     7081 aatgggtgaa tatagtgcag aacaaatcag aaacggtgcc acaccggttc cttttgatat
     7141 ttcacttcaa aactgcgttc gtgtgactaa tattgagaca aaattagttt caacaaaggt
     7201 tggcactgaa aacgggcaac tccttggtaa tactctcaca ggtaatgacg cagcaaaagg
     7261 agtcggtgta ctcatagaag gtttagcaac tagtaaaaat cctctaatga cattgaaacc
     7321 taatgattca aattctgttt ataaagatta cgacccgaga ggcaaagacg atacaacagg
     7381 aggagtttac ccggatcaag atacaggtat aacataccct ctccatttcc aggccacgct
     7441 acagcaggat ggaactatac caatagaagc tggtgagttt aaagccacca gtactttcca
     7501 ggtaacctac ccttaataag cccaccgcac cgccatcatc tgacggtgcg gtaattgata
     7561 aatcattgcc cccaccaatg tgactgataa aatgccaggc aactcgacat catttatcag
     7621 gatacgttat gaaaccgacc accattgctt cacttcagaa atgtaagcag gataaaaagc
     7681 gtttcgcgac catcaccgct tacgactaca gcttcgcgaa actatttgct gatgaaggac
     7741 ttaacgtcat gctggtaggc gactcgctgg gcatgaccgt tcagggtcac gactccaccc
     7801 tgccggtgac cgttgccgat atcgcctacc acactgcctc cgtacgtcgt ggtgcgccaa
     7861 actgcctgct gttggctgac ctgccgttta tggcgtatgc cacgccggaa caagcctttg
     7921 aaaacgccgc aacggttatg cgtgccggtg ccaatatggt caaaattgag ggcggtgagt
     7981 ggctggtcga aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt ggtcacttag
     8041 gtttaacccc acagtcagtg aatattttcg gtggctacaa agttcagggg cgcggcgatg
     8101 aagcgggcga tcaactgctc agcgatgcat tagccttaga agccgctggg gcacagctgc
     8161 tggtgctgga atgcgtgccg gttgaactgg caaaacgtat taccgaagca ctggcgatcc
     8221 cggttattgg cattggcgca ggcaacgtca ctgacgggca gatcctcgtg atgcacgacg
     8281 ccttcggcat taccggcggt cacattccta aattcgctaa aaatttcctc gccgaaacgg
     8341 gcgacatccg cgcggctgtg cggcagtata tggctgaagt ggagtccggc gtttatccgg
     8401 gcgaagaaca cagtttccat taaggagtca cgttgtgtta attatcgaaa ccctgccgct
     8461 gctgcgtcag caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc tggtgcctac
     8521 tatgggtaat ctgcacgatg gccatatgaa gctggtcgac gaagccaaag cccgcgccga
     8581 tgtggtcgtc gtcagtattt tcgttaaccc gatgcagttc gaccgcccgg aagatctggc
     8641 acgttatcca cggaccttgc aggaggactg cgagaagcta aacaaacgta aagtagattt
     8701 agttttcgcc ccttcggtaa aagagatcta cccgaacggt acagaaaccc acacctatgt
     8761 tgatgttcct ggcctttcga ccatgctgga aggtgccagc cgtccgggac attttcgcgg
     8821 cgtttcgact attgtcagca agctgttcaa cctggtccag ccagatatcg cctgcttcgg
     8881 tgaaaaagat tttcagcaac tggcgctgat ccgcaaaatg gttgccgata tgggcttcga
     8941 tattgagatt gtcggtgtgc caattatgcg cgccaaagac ggtctggcgc tgagttcccg
     9001 taacggttat ctgacggcgg aacaacgcaa aatcgcgccc ggtctgtaca aagttttaag
     9061 ttcgattgcc gacaaattgc aggctggaga acgggatctc gatgaaatta ttgctattgc
     9121 cgggcaagaa ctgaatgaaa aaggcttccg cgccgatgat attcagattc gtgatgccga
     9181 cacgttgctg gaagtctctg aaaacagcaa acgggcagtg attctggtag ccgcctggct
     9241 tggcgatgcc cgcctgatcg acaataaaat agtcgagctg gcgtaatact taactggcgc
     9301 tacggctgat ggcgccagtt attaatttac cccacgtcat ctgtcataac acaggtaact
     9361 taaataaggt taattacctt atcaatctca gcaagagata aatttgtcat ctctgcaacg
     9421 tcttcccgag acattccttt acttagaaga cgcagggcga attcttgcct ttcctcctgt
     9481 cttccctgct gaatcccctt ctcaatccct ttctcttcaa accactgcgc cagcgtcatc
     9541 atagactccc ctcccgtttc cctgtctctc agcacaccat aaaacaaatc cgcttgttca
     9601 gtatgaccgc gttgcagcat atagttttgc atggcaacta actgacttcc gctagtgtac
     9661 ccttcgtcaa tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg ctggcgaata
     9721 tgtttttgca gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt
     9781 gtgatggtga tatccaccag cgggaaagga ctgttataga cgcgtcgtgc cagctccggc
     9841 gagtaaaata tatcaaacca gcacattgag agcggataag gtgtggcctc gccctgataa
     9901 aacagtatcg gcaccaccag cggcagctta tcgtgatccg cctccagatg ccggtgcatg
     9961 gcagcaatag aataacgcat catgcgaaag gccattttct tatccggctt gctttggtgt
    10021 tcaatcacaa catgcagata accgggattg ccctgcattt gcacggaata gagcacgtcc
    10081 gtgctgtgtc ctttcaggct ctcttcaatg aaactccccg actctaaatg aagcgtgttg
    10141 aggtcacaaa gttctcgtaa ttccactggc aaatgtatct ccagaaagtc gcgagccgtc
    10201 tccgcatgca ttaaaaattg tttaaatacc gcgtcatgcg gtgtggtact cggtgcatcc
    10261 atcttccctg tcttccgtgt tgtcttgcgt gaaggcagta tccagtcaag gcgcgccgtc
    10321 gtcagccgcc tctttagtgt ttaccgagca gcgttcagag aaatttcaat tcgttacagg
    10381 cgatacatgg cacgcttcgg cgcggtacgc aaactgctga cgcaaatgcc atcagatgag
    10441 tagacactca tcaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc aggcaccgcc
    10501 tgcttcgtta acgacagggt agaaaggtag aagttatgat tcgcacgatg ctgcagggca
    10561 aactccaccg cgtgaaagtg actcatgcgg acctgcacta tgaaggttct tgcgccattg
    10621 accaggattt tcttgacgca gccggtattc tcgaaaacga agccatcgat atctggaatg
    10681 tcaccaacgg caagcgtttc tccacttatg ccatcgcggc agaacgcggt tcgagaatta
    10741 tttctgttaa cggtgcggcg gcccactgcg ccagcgtcgg cgatattgtc atcatcgcca
    10801 gcttcgttac catgccagat gaagaagctc gcacctggcg acccaatgtc gcctattttg
    10861 aaggcgacaa tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt
    10921 tatccctgcg gctggttact caccagccgc gacatcgtct ccagcgaatc cgttcttaag
    10981 atataaagtc gttttagtaa caacggatta tcccccggtt ttactttgcc tttcatggtt
    11041 gtcaccgcca ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc attaaatccg
    11101 ccaaacggat acgaaagata ccagacatgc ggattaaatt gcgccagagc gcggcgtgaa
    11161 cgtgcaaaat caaacagaat attgtgctca ctacggctca gtaatatggg tcggcgatag
    11221 ccatccaccc gatgcaaaaa atgggtatgt gactgaaaat caaaaacatc gcgaatttcg
    11281 ttaagctcag aaacgctcat aaattgcagc gattttgggt tccacttctg cgggtgacgt
    11341 ttgatgcgcg aggtaacaac aaacgccgtc gccttcatgc catattgttt caatacagga
    11401 tacgcatagc ggctcaccga cttgaggcca tcatcaaagg taatcaccac cgctcgcgca
    11461 gggagattga tcttattctt cacgtaacct tccagctgcg ccatgctcag tgtcgcgtat
    11521 cccctgtcgc gcagccaggc catctggtta ttgaaagcgc gtaccgaggt ggtcgtcgaa
    11581 gtatggcgaa aacgggtgtt ttcttcatcg cgcagaatat ggtgataggt tagcaccggc
    11641 aggccattat cgggttgggc atccagcgcg ctgatatagg cgagtcgatc gccaatacgg
    11701 atctgatacc aggtttgatt taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa
    11761 ttgtccgcca gtaccccaaa tggcgcactg cccgcactcg gcgcgttata gaccggcgta
    11821 tctttccagg taattaagtt ctgattactc agcggcttgt tgaggtcacc caaaccgtct
    11881 tcaacttttt gtcgcccctg aactggctcg agatgccctt tatcgataaa ccctttacca
    11941 aagccaaaat taaatgcgta ataacttgcg gcagtgggct ccacggcaat gatttgcccg
    12001 gcccgaatat tccccacggt caccatcttg tcgccaattt gcgcccagat cgcagcattt
    12061 tcgatggttt gcatataacg ggcaggtaac gcggcactga cacttgcggt aaacagcatc
    12121 agcaggagaa taacagcttg tttgtacatg aaaaaataac cgagggtgaa acaacgcttt
    12181 tattgtagca aaagcgctat aagaatcgtg ggttaatact catacaaatt atgtttgagg
    12241 acgaaaggcg gatttttttg ttgttggtgc caaagactac tcacctccgg cccacccagt
    12301 tcgacaatac gctcgcgaaa ctctgaactg gtcatggttt cacggcaagc catcatctgt
    12361 tcaattaacg gtaacgtgac accagaaatc acttcgcaac gggagtgttt gtggcttaat
    12421 aatgaagcca cgcggtacgg tggcgctccg gctatatcgg ttaagaagat gacaccctca
    12481 ccagagtccg cttcatgcag agcatcgcac atcatgcggc tgagcatatt agagcttaac
    12541 ccacgccaga aattcacggc ccggcactga agaagcgccc catgtttttt ctccagcgca
    12601 tccagtatct cttgcgcccg gtcatcgtga caggtaatta cccaacctaa catcgccttc
    12661 ctccttagcc gaaaatcagt ctaccctgac cattgttgat aaagggtgat agccgtcaaa
    12721 actcctctcc cccaaatcca gaggagagga aatagcctta gctacgcaaa ccacgtccac
    12781 gttggatcag cgaccaacag atcaaataaa acgccacaat aaagaccacc agtacgccaa
    12841 aggtagtgac cagcggaaca tcattgatac cgaggaagcc gtagcggaaa ccactgatca
    12901 tataaacgat tgggttcagg tgcgacagcc cttgccagaa cggcggcaac aaagtcagtg
    12961 agtaaaagac cccacccaaa tacgtgagtg gcgttaacac aaaggttggc accaggctga
    13021 tgtcatcgaa cgttttggca aacacgccgt tcagcaaacc cgcaagggag aatagcaccg
    13081 ccgtgagcac cagggttaag gcaacgaata cccacgaatg cacctgaaac ggcacaaaaa
    13141 acagtgaaat tgccgtcacc agaatgccaa caaacagacc acgcgccacg ccaccgccga
    13201 catatccggc aataatgacg tgagtcggaa ccggcgctac cagcagctct tcaatattac
    13261 gctggaactt ggcaccaaaa aatgatgacg cgacgttagc gtaggcattg gtgatcaccg
    13321 acatcatgat cagccccggt acgatgaact gcatatagct gaagccatgc atatcgccaa
    13381 tacgcgaacc tatcaggtta ccgaagataa taaagtaaag ggtcatggtg atgactggcg
    13441 gcaccagcgt ctgcacccag atacgcataa agcgatggat ctctttcgcc cagatgcttt
    13501 ttagcgccac ccagtaaaga tgcatcatgc gcgatctcct tgtttttcat taaccagtga
    13561 aacaaacagc tcttccagac ggttagcttt gttacgcata cttaatacct gaatgccctg
    13621 ctcacttaac tgcgtaaata cgctgttgat cccctgctca cgcagcactt caacttccag
    13681 cgtcgcggta tcgaccagtc gatactgata gccatcgagc ttcggtaacg gacttttcgg
    13741 tgcgagatcg agaataaagg tttccgattt cagcttagcc agcagcgcct tcatcgaggt
    13801 attttccacc agctcaccgt gttgaataat gccgatattg cggcacagca tttctgcttc
    13861 ttccaggtag tgtgtggtga gaatgatggt ggtgcctttg tcgtttaaat ccttcaaaaa
    13921 gccccacatt gagcggcgaa gttcaatatc cacgcctgcg gtcggttcgt cgagaatcag
    13981 tagtttaggt tcatgcatta acgcacgggc aatcattaaa cggcgcttca tcccgccaga
    14041 taacatacgc gcacgttcgt tgcgttttcc ccatagatcg agttgtttaa gatatttttc
    14101 gctgcggatg tacgcctctt tgcgctccac accgtagtac cctgcctgat ttaccacaat
    14161 ttgctgcacg gtttcaaacg ggttgaagtt aaattcctgc ggcaccagtc ccaactgacg
    14221 tttagcgttc acgacatctt tctcgagatc gtaaccaaat acgctgaccc gcccggaggt
    14281 tttatttacc agagagctga taataccgat agtggtcgat ttcccggccc cgttcggccc
    14341 gagaagcgca taaaaatcac ccgcttcgac ctgcaaatct attccacgaa gcgcctgaac
    14401 gccgcctgga taagtttttt taagctgttg aagttccagt gcaatggtca taaattttta
    14461 cttaccttac gttcttacac tttatctgtg gtttaaatcg tcccggagtt gccctatatt
    14521 agccaaacgt aattatttgg ttacaggtcg ttaacctcca tgaaagacat agatacactc
    14581 atcagcaaca atgcactatg gtcaaaaatg ctggtggaag aggatcccgg gttttttgag
    14641 aaactggcac aagcgcaaaa accgcgcttt ctatggattg gatgttccga cagtcgcgtt
    14701 cctgcagaac gtttaaccgg tcttgagccg ggcgaactct ttgttcaccg taatgttgct
    14761 aacctggtca ttcacaccga cctgaactgc ctttccgtgg ttcagtatgc agtggatgta
    14821 ctcgaagttg aacacattat tatctgtggc cactacggtt gcggcggcgt acaagccgca
    14881 gttgaaaacc cggaactggg gcttatcaac aactggctgc tgcatatccg cgatatctgg
    14941 ttcaaacata gctcattgct cggcgaaatg ccgcaagagc gccgtctgga taccttgtgt
    15001 gaactgaacg tcatggaaca ggtttataac ctgggccact ccaccattat gcaatcagcg
    15061 tggaaacgcg ggcagaaagt taccattcac ggctgggcct acggcattca cgacggcttg
    15121 ctgcgtgatc tggatgtcac agcgaccaac cgcgaaaccc ttgagcaacg ttaccgtcac
    15181 gggatttcca acctcaagct gaaacacgcc aaccacaaat aaaaatgcca tgccggatgc
    15241 aacacatccg gcaacttcac acttactcgt ccagcagaat cactttgcag atatacggca
    15301 gatgacggta acgctgtgcg taatcaatgc cgtaacccac cacaaactca tccgggatcg
    15361 agaaaccgat aaattcaacc gggacgttca cttcacgacg ggacggttta tccagcagcg
    15421 tacaaatcgc cagcgacttc ggttcgcgca ggcttaagat ctcacgcact ttcgacagtg
    15481 tattccccga gtcgatgata tcttcaacaa tcagcacgtc cttgccacgg atatcttcat
    15541 ccagatcttt gaggattttc acatcacggg tggtggacat gccgctaccg tagctggagg
    15601 cggtcataaa gtcgacttca tgagatacct gaacttcacg gcacaggtcc gccataaaca
    15661 taaatgagcc acgcagcaga ccaaccagca ccatatcgct gccgctgtct ttgtaacgct
    15721 cagtaatctg acgacccagt tcggcgatac gcgctttaat ctccgcttcg gggatcatta
    15781 cttctacagt atgtttcata tctctaacca tatgatttaa aaacaaatca cttcgcacgg
    15841 gcaggttttg gcaccgcaac aaagcgcaag acacgcagta taccagtaaa actattcatt
    15901 cgcggaatct gttaataaag ctcgaattgt gatgacgatc acacatgtta aaacctataa
    15961 ttaattgcta ctaaaatatt aatgaattga aatggtgtct ctttatggca attaacaata
    16021 caggctcgcg acgattactc gtcacgctaa cagccctttt tgcagcgctt tgcgggctgt
    16081 atctactcat tggcggaggc tggctggtcg cgattggcgg ctcctggtac taccctatcg
    16141 ctggtcttgt gatgctcggc gtcgcctgga tgctgtggcg cagtaaacgt gccgcgcttt
    16201 ggctgtacgc agccctgctg ctcggcacca tgatttgggg cgtctgggaa gttggtttcg
    16261 acttctgggc gctgactccg cgcagcgaca ttctggtctt cttcggcatc tggctgatcc
    16321 tgccgtttgt ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc gcactggtgg
    16381 tggcactgct gattagcggt ggtatcctga cctgggccgg atttaacgat ccgcaggaaa
    16441 tcaacggcac cttaagcgcc aatgccacac ctgcggaagc tatctcaccc gttgccgatc
    16501 aggactggcc tgcctatggt cgcaatcagg aaggtcaacg cttttcacct ctgaaacaaa
    16561 ttaacgccga taacgtccat aatctgaaag aagcctgggt gttccgtact ggcgatgtga
    16621 agcagccgaa cgatccgggc gaaatcacca atgaagtgac gccgattaaa gtgggcgaca
    16681 ccctttacct gtgtaccgct caccagcgcc tgtttgcgct cgatgccgcc agcggcaaag
    16741 agaaatggca ttacgatcct gagctgaaaa ctaacgagtc tttccagcac gtaacctgcc
    16801 gtggcgtctc ttatcatgaa gccaaagcag aaacggcttc gccggaagtg atggcggatt
    16861 gcccgcgtcg tatcattctt ccggtcaatg atggtcgact gattgcgatt aacgctgaaa
    16921 acggcaaact gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa agcaatatgc
    16981 cagacaccaa accgggtctg tatgaaccga cttcgccacc gattatcacc gataaaacca
    17041 tcgtgatggc cggttcagtt accgataact tctcaacccg cgaaacgtct ggcgtgatcc
    17101 gtggttttga tgtcaacacc ggggagctgc tgtgggcttt tgatcccggc gcgaaagatc
    17161 cgaacgcaat cccgtctgac gaacacacct ttacctttaa ctcgccaaac tcctgggcac
    17221 cagcggccta tgacgcgaag ctggatctgg tctatctgcc gatgggcgtg accacgccgg
    17281 atatctgggg cggtaaccgc acaccggaac aggaacgtta tgccagctcg attctggcgc
    17341 tgaatgccac taccgggaaa ctggcgtgga gctaccagac cgttcaccac gacctgtggg
    17401 acatggatct tccggcacag ccgacgctgg cggacatcac cgttaatggt cagaaagtgc
    17461 cagttattta cgctccggcg aaaaccggca acatttttgt gctcgatcgt cgtaatggcg
    17521 aactggtggt tccggcaccg gaaaaaccgg ttccccaagg tgctgccaaa ggcgattacg
    17581 ttacccctac tcaaccattc tcggaactga gcttccgtcc gaagaaagat ttgagcggtg
    17641 cggatatgtg gggagccacc atgtttgacc aactggtgtg ccgcgtgatg ttccaccaga
    17701 tgcgctatga aggcattttc accccgccat ctgaacaggg tacgctggtc ttcccgggta
    17761 acctggggat gttcgaatgg ggcgggattt ccgttgatcc aaatcgtgaa gtggcgattg
    17821 ccaacccaat ggcactgccg tttgtttcga aactgatccc gcgtggtcct ggtaacccga
    17881 tggagcagcc gaaagatgcc aaaggcacgg gtacggaatc cggcattcag ccacagtacg
    17941 gtgtaccgta tggtgtcacg ctcaacccgt tcctctcacc gtttggtctg ccatgtaaac
    18001 agccagcatg gggttatatc tcggcgctgg atctgaaaac caatgaagtg gtgtggaaga
    18061 aacgtattgg tacgccgcag gacagtatgc cgttcccgat gcctgtaccg gtgccgttca
    18121 atatgggtat gccgatgctg ggcgggccaa tctccacggc gggtaacgtg ctgtttatcg
    18181 ccgctacggc agataactac ctgcgcgctt acaacatgag caacggtgaa aaactgtggc
    18241 aaggtcgttt accagcgggt ggtcaggcga cgccaatgac ctatgaagtg aatggtaagc
    18301 agtatgtggt gatctccgca ggcggtcacg gttcatttgg tacgaagatg ggcgactata
    18361 ttgtggctta tgccctgccg gatgatgtga agtaagactt gctcagattg ctgacaacgt
    18421 gcgcgttgtt catgccggat gcggcgttaa cgccttatcc agcctacaaa attgtgcaaa
    18481 atcaatggat tgcacaggta
//
LOCUS       DAZGSP010000098        18492 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZGSP010000098 DAZGSP010000000
VERSION     DAZGSP010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.099"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            100..795
                     /gene="yafL"
                     /locus_tag="ACHK74_003831"
     CDS             100..795
                     /gene="yafL"
                     /locus_tag="ACHK74_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP0222572.1"
                     /translation="MPFSSFASATTSHITFSYAARQRMQNRARLLKQYQTHLKKQASY
                     IVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITE
                     VAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAKLPRTANEMYHYHRATI
                     VANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRLAEPFWQDHF
                     LGARRILTEETIL"
     gene            complement(852..1208)
                     /locus_tag="ACHK74_003832"
     CDS             complement(852..1208)
                     /locus_tag="ACHK74_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP0222573.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAHM"
     gene            complement(1201..1479)
                     /locus_tag="ACHK74_003833"
     CDS             complement(1201..1479)
                     /locus_tag="ACHK74_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFP0222574.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            complement(1584..3296)
                     /locus_tag="ACHK74_003834"
                     /pseudo
     CDS             complement(1584..3296)
                     /locus_tag="ACHK74_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            3268..4053
                     /gene="lafU"
                     /locus_tag="ACHK74_003835"
                     /pseudo
     CDS             3268..4053
                     /gene="lafU"
                     /locus_tag="ACHK74_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
     gene            4124..5179
                     /gene="dinB"
                     /locus_tag="ACHK74_003836"
     CDS             4124..5179
                     /gene="dinB"
                     /locus_tag="ACHK74_003836"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFP0222575.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            5176..5628
                     /gene="yafP"
                     /locus_tag="ACHK74_003837"
     CDS             5176..5628
                     /gene="yafP"
                     /locus_tag="ACHK74_003837"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0222576.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            5874..7013
                     /locus_tag="ACHK74_003838"
     CDS             5874..7013
                     /locus_tag="ACHK74_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFP0222577.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALIGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            7010..7624
                     /gene="prfH"
                     /locus_tag="ACHK74_003839"
     CDS             7010..7624
                     /gene="prfH"
                     /locus_tag="ACHK74_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFP0222578.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(7681..9138)
                     /gene="pepD"
                     /locus_tag="ACHK74_003840"
     CDS             complement(7681..9138)
                     /gene="pepD"
                     /locus_tag="ACHK74_003840"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP0222579.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            9399..9857
                     /gene="gpt"
                     /locus_tag="ACHK74_003841"
     CDS             9399..9857
                     /gene="gpt"
                     /locus_tag="ACHK74_003841"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP0222580.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            9949..11193
                     /gene="frsA"
                     /locus_tag="ACHK74_003842"
     CDS             9949..11193
                     /gene="frsA"
                     /locus_tag="ACHK74_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP0222581.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            11251..11652
                     /gene="crl"
                     /locus_tag="ACHK74_003843"
     CDS             11251..11652
                     /gene="crl"
                     /locus_tag="ACHK74_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP0222582.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(11691..12746)
                     /gene="phoE"
                     /locus_tag="ACHK74_003844"
     CDS             complement(11691..12746)
                     /gene="phoE"
                     /locus_tag="ACHK74_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFP0222583.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            13034..14137
                     /gene="proB"
                     /locus_tag="ACHK74_003845"
     CDS             13034..14137
                     /gene="proB"
                     /locus_tag="ACHK74_003845"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFP0222584.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            14149..15402
                     /gene="proA"
                     /locus_tag="ACHK74_003846"
     CDS             14149..15402
                     /gene="proA"
                     /locus_tag="ACHK74_003846"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFP0222585.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            15517..15592
                     /locus_tag="ACHK74_003847"
     tRNA            15517..15592
                     /locus_tag="ACHK74_003847"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15550..15552,aa:Thr,seq:cgt)
     gene            15756..16964
                     /locus_tag="ACHK74_003848"
     CDS             15756..16964
                     /locus_tag="ACHK74_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0222586.1"
                     /translation="MKLNARQVDAAKPREKAYKLADGAGLYLEVVPSGSRYWRMKYRF
                     NGKEKRMAFGVYPAVSLAQARALRDDAKKKLAEGIDPSLAKKEEKLVRDVQLNNTFQA
                     VALEWHGTKVSRWSEGYASDIIEAFNKDIFPYIGQQPVNEIKQLVLLNVLRRIESRGA
                     TEKAKKVRQRCSEVFRYAIVTGRAEYNPAADLTSAMSGHESKHYPFLTVEELPDFFKA
                     LAGYTGSPLVVLAARLLILTGVRTGELRGAFWSEFDLEKAVWEIPAERMKMKRPHLVP
                     LSSQALEIVQQLKVMSGQYPLVFPGRNDPRKTMSEASINQVFKRIGYTGRVTGHGFRH
                     TMSTILHEEGFNTAWIETQLAHVDKNAIRGTYNHALYLEGRREMMQWYADYINANHNS
                     CISLLVNATK"
     gene            17206..18414
                     /locus_tag="ACHK74_003849"
     CDS             17206..18414
                     /locus_tag="ACHK74_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP0222587.1"
                     /translation="MNSNYMPFTQRDSLGRYYTKESISALLVSQMKAEKVNNIIDLAS
                     GEGSLTYAALDRWKNAEAYSLDIESRMSKKVCDNLTHIVTDALVHSFPEMLARHQGNF
                     DVAVCNPPFTLPEWRDDYFKIISEIGADKYISVSKYVPAEIIFISQVIRFLKKGGEAG
                     IILPDGIFTARKFIGLRRYLLNEHSITKVIELPRNIFKRTEAKTHILIFNKKIMPHHK
                     IQLHCITKDGELSPPVLIRKEDAVERMDYSYHYNKNEGKGFSTIGMLKNISIFRGRFN
                     SKEITEHVFHTTKFSGDEKYIKFHCNSVEELKPSKLDVIAKPGDILIARVGRNFHKKI
                     LFVESGYSYISDCIFLIRASGGDKKKLFDFLCSQDGQEELSRASSGVAAQHITMDALK
                     KIHLVRIKHD"
BASE COUNT         4659 a         4439 c         4768 g         4626 t
ORIGIN      
        1 ccgggataca ctgcaacatc aatgcatgat cagccattat tgagtatgtc cttgccgtcg
       61 attccctctt ttgtgttgtc gggattactg ttgctttgct tgccgttttc ttcatttgcc
      121 agcgccacca catcacatat cactttcagc tacgccgccc gccagcggat gcaaaatcgt
      181 gcgcgtttat taaaacagta ccaaacccat ctgaaaaaac aggccagcta tattgtggaa
      241 ggcaatgccg aaagcagaag ggcgctacgc cagcacaacc gcgaacagat aaaacagcat
      301 ccagaatggt ttcctgcgcc gctcaaggcg agtgacagac gctggcaggc gctggcggaa
      361 aacaaccatt tcttaagcag cgaccatctg cataacatca ccgaagtagc gattcaccgt
      421 ctggagcaac agcttggcaa gccttacgta tggggcggca cgcggcccga tcaaggcttc
      481 gactgtagcg ggttggtttt ctatgcctac aacaagatcc ttgaggcgaa gctcccacgc
      541 acggccaacg agatgtatca ctaccatcgg gcaacgattg tggcgaataa cgacctgcgc
      601 cggggagatt tgctgttttt ccatatccac agccgcgaga ttgccgatca tatgggggtg
      661 tatctgggcg atggccagtt tatcgagtcg ccgcgcaccg gggaaaccat tcgggtaagc
      721 cggttagccg aacctttctg gcaggaccat tttttgggcg cgcgcaggat tttgacggaa
      781 gagacgattt tgtaaatggg taatattgac gaactggcaa aaggccggat gccacgcggc
      841 atccgacttg tttacatatg ggctgaaaac tcacgttcta agacatcaac aatgagctgg
      901 ttttttgaaa tggaatgttg tctggcgacg atggtcagcc gcgaatcaat gtgatgagga
      961 acacgaagcg tcaggcgttt ttgctcttct tcttcacgaa aaggggcaat accgtcctct
     1021 tcacaatccg ccatccactc atccagcgcg ctttcgccct cacgatataa tgcctcaatg
     1081 cttgtggcta caaagtcaca ataaccgttt acattgagga acttgccgcg aaatgcgttg
     1141 atatcggctt caaatgtcac tgcggctggt tgacctttaa ttgtgataat gttcgatgct
     1201 ttattcataa tctaccccta cacagttaac aaaccactcc ttaagacctg ctactgcacc
     1261 tttatccatc acattctttg gatgtggctc atgtgtactg aatttggtcg ttccaatctg
     1321 aaatttcctt ctggacccat tgttattttt tattgttcca cccaccgctt taatgagcgc
     1381 aacaacatca tcccatttaa ttccagattt tggcggctcc gtcattacat cgtcgaatgt
     1441 ttttctatgg cgggagccta gcgcgctgac cttaatcata ctatcaatcc atgacgtcat
     1501 tgtatggtgt catcgtagta tgtttgcaat gtgacgtcaa tcaatggtgt cagatgcgtg
     1561 caaagcatgg aaaagaatcc tcatcaactc aacgctccca taatcttcaa ctccagctca
     1621 tccggcactt cattatacga cagcacatgc agccccggcg caaacaacct cgcatagcgt
     1681 gccagcaaag ggcgcaactg cggcggcacc agcagcaccg ggtctttccc cgccgctttc
     1741 atctgctcct tcacctgcgg catcgtactc tggaactggt tgagcatatt cgggtcgacc
     1801 ggcacgctgt cgagcatcac tttgccgccc tgttgcgcct gattcaccac gttggtcagc
     1861 agattttcca gttcattatt cagcgtatat accgtcagct cctgcttgtg aacgaacgga
     1921 tgggtaatgc tgcgccgcaa tgccaggcgc acatcggccg ccagcagaat atgatctttg
     1981 gtcaccgcgc tactggcgac cagcacggtg gcgatggtga cgatatcgcg cagggaaacg
     2041 ccttcggtca gcaacgcccg gtacactttc agcaactggc tgtaattgag cgccgcgctt
     2101 aagtcttccg ccaggcgcgg ggccatcgac gacaggcggt tatgcagttg cgtaatgtca
     2161 tcatagttaa acaaatcagg aatatagctg cgcacaatct tgtttacatg ggtggcaatt
     2221 acgctggcgc tgtcgatcac ctgataaccc atattcagcg ccttcgcttt ctgcgcaggc
     2281 tggatccagg tcaccggcat cccgtacgcc gggtcgttcc ccaatacgcc gtcaatctcg
     2341 ccgtaggttt cgctggaggg cagggccatc agtttatccg ccggaatatc cgcttcatca
     2401 gcctttatgc cgttgatgaa aatggcgtac tggctgggct tgaggcggaa gttttcccga
     2461 atgcggatct ccggcaacaa tacgccgttg ccgtcagaaa tcacctgccg cacgccgcga
     2521 atccgctggg tgagcgggtt accctgcgct ttatcgacca gtgccaccag cttgtagccg
     2581 aggcttaaac tgataggctc gatcagcggt attgtctccc aactgacttg ttgctcgctg
     2641 gtttcagtga tggtgcgggt cagcgtttcg aggcttttct cttccgcctc tgccgcctgc
     2701 gggcgtttgc tcatccgcca gccggtaaag ccaagcaggg cgctgaacag caaaaacggc
     2761 agatgcggca tccccggcac caccgccagc acaaacataa ttccggcggc ggtgtagagc
     2821 accgacgggc ttgccagcag ctggttacgc acatcatggg cgatatcgcc gttgtcgctg
     2881 acgcgggtga cgataattgc cgccgcggtg gagagcagca gggaagggat ctgcgccacc
     2941 aggccgtcgc cgatggtcat cagcacatac tgctggaagg cagcatcggc gctcaggttg
     3001 tatttgaaga tcccgataca gacgccgccg atcaggttga tcgccagaat catcatcccg
     3061 gcgatggcgt ccccgcgcac aaacttcgat gccccgtcca tcgcgccgta gaaatcggcc
     3121 tcgctggcaa catctttacg ccgggtttgt gcctgtgcct ggttgatcaa cccggcgtta
     3181 agatcggcat caatcgccat ctgtttaccg ggcatcgcgt ctaaggtaaa gcgggcagaa
     3241 acctcggaaa tacgctcggc ccctttggtg acgacgataa agttgattgt caacagcgtc
     3301 agtaaatccg agcgtgaaag cattatcgcc gcgctgcacg ggcagtcgat ttttaacggt
     3361 ggtgggctgt cgccgttaaa taaaatcagc ccgtctcatc cgccaaaacc tgccaccgtg
     3421 gcggtgccgg aagagacgga gaaaaaagcg cgtgacgtca atgagaaaac ggtgctgctg
     3481 aagaagaaat ccgccaccga gcttggcgag ctggcaacca gtatcaacac cattgcccgt
     3541 gatgcgcata tggaagcgaa tctggagatg gagattgtcc cgcagggata acgcgtgctg
     3601 attaaagacg accagaatcg caatatgttc gaacgcggca gcgcgcagat tatgccgttc
     3661 tttaaaaggc tgctggtgga gctcgcgcca gttttcgact cgctcgataa caaaattatt
     3721 attaccgggc ataccgatgc gatggcctac aaaaacaata tctacaacaa ctggaacctt
     3781 tcgggcgatc gcgcgctttc agctcgtcgg gtgctggaag aggccggaat gccggaagat
     3841 aaagtgatgc aggtaagcgc aatggcggac cagatgctgc tggacgcgaa aaatccgcaa
     3901 agcgcgggca accggcgcat tgagattatg gtgctgacca aaagtgcgtc cgatacgctg
     3961 tatcaatact ttggtcagca tggggataaa gtggtgcagc cgctggtgca aaagctggat
     4021 aagcagcagg tgctttcaca gcgaacgcgc tagatgctga atctttacgc atttctcaaa
     4081 ccctgaaatc actgtatact ttaccagtat tgagaggtga gcaatgcgta aaatcattca
     4141 tgtggatatg gactgctttt tcgcagcggt ggagatgcgc gacaatcccg ccctgcgcga
     4201 tatccctatt gctattggcg gcagccgcga acgtcggggg gtgatcagca ccgccaatta
     4261 tcccgcgcgt aaatttggcg tacgtagcgc tatgccgaca gggatggcgc tcaaattatg
     4321 cccgcatctc accttgcttc cggggcgctt tgacgcctac aaagaagcct caaatcatat
     4381 tcgcgaaatt ttctcgcgct acacctcgcg cattgaaccg ttgtcactgg atgaagccta
     4441 tctcgatgtc accgatagcg tccattgcca cggttctgcg actctcatcg cccaggaaat
     4501 ccgccagacg attttcaacg agctgcaact gacggcatct gcgggcgtgg caccagtaaa
     4561 gtttctcgcc aaaatcgcct ccgacatgaa taaacccaac ggtcagtttg tgattacgcc
     4621 ggcagaagtt ccggcatttt tacaaacctt accactggca aaaatccccg gcgtcggcaa
     4681 agtctcggcg gcaaaactgg aagcgatggg gctacgaacc tgcggtgatg tacaaaagtg
     4741 tgatctggtg atgctgctta aacgctttgg caaatttggc cgcattttgt gggagcgtag
     4801 tcaggggatt gacgagcgcg acgttaacag cgaacggttg cgaaaatccg tcggcgtgga
     4861 acgcacgatg gcggaagata tccaccactg gtctgaatgt gaagcgatta tcgagcggct
     4921 gtatccggaa cttgaacgcc gtctggcaaa ggtgaaacct gatttactga ttgctcgcca
     4981 gggggtgaaa ttaaagtttg atgattttca gcaaaccact caggagcacg tctggccgcg
     5041 gctgaataaa gctgacttaa tcgccaccgc gcgtaaaacc tgggatgaac gccgcggcgg
     5101 gcgcggtgtg cgactggtgg ggctgcatgt gacgttgctt gacccgcaaa tggaaagaca
     5161 actggtgctg ggattatgaa taacatacaa ataagagact atcagcctgg cgattttcag
     5221 caactatgcg ctattttcat tagagcggtt acgatgactg ccagtcagca ttattcacca
     5281 caacaaattg ccgcctgggc gcagattgac gaatctcgct ggaaggagaa actcgcgaaa
     5341 tcacaagtgc gggttgcagt cattaatgca aaactggtcg gctttattac ctgcgttgaa
     5401 cactatatcg atatgttatt tgttgaccct gaatacaccc gccgtggggt tgccagcgct
     5461 ttgttaaaac ctttgattaa gtctgaatcc gaacttacgg tggacgcaag cataaccgca
     5521 aaaccctttt ttgaacgtta tggttttcag acagttaagc agcaacgcgt tgaatgtcgg
     5581 ggagagtggt ttattaattt ttatatgcgt tataaacagc aacattaaat ccagcttgca
     5641 atgaaaataa cgcaagccta gtatgtgcgc gttttcttat tccacaaact gcaaggaggt
     5701 aaatcatgac aaatccttta tcaatgactc tttgcagacc tttccaggat taattctttt
     5761 tttcttgccc tggattcgtc tgccatttcc tgatttttta attgttactc tcaataattc
     5821 aagtcgcaac acatgcaact tgaagtatga cgagtctatt tacggaatgg tatatgggca
     5881 attatattcg tcccttatcc gatgcggtat ttaccatcgc atctgatgac ctgtggatcg
     5941 agagtttagc gatccaacaa ttacacacca cggcaaattt acccaacatg cagcgcgtag
     6001 tggggatgcc agatttacat cccggacgcg gctatccgat tggcgcagcg ttcttctctg
     6061 ttggtcgttt ttatccggca ctgatcggca atgatatcgg ctgcggtatg gcgctatggc
     6121 aaacagatat tctcactcgc aaatacaacg ccgataagtt tgaaaagcga ttatcagatc
     6181 tggatgacgt tgctgaagaa agctggctgg aggaaaacct gccgtcagca tttgcacagc
     6241 atccgtggcg cagctcgctt ggctctatcg gtggcggcaa tcatttcgct gaactgcaac
     6301 aagttgatca aatcatcaac gctgaactgt ttgcactggc aggtctggat gcacaacatc
     6361 tgcaactgtt ggttcatagc ggctcgcggg gggtggggca gtctatttta cagcggcata
     6421 ttgcctcgtt ctcgcatcat ggtttgcctc aaggcagtga cgacgcgcta cggtatattg
     6481 cggaacatga cgatgcgctg gcgtttgcgt gtattaatcg ccagatgatc gctttgcgca
     6541 taatgcaaca agttaaggcc acgggaagtc cggttctgga tgtggcgcat aactttgtta
     6601 gcgcgtgtca aatcggcgat caacagggct ggttgcatcg taaaggtgcc acgccggatg
     6661 acaacggtct ggtgattatt cccggttcac gtggtgatta ctcctggatt gtacagcctg
     6721 ttgcgaacga aaaaacgctc cactcgctgg cgcatggggc agggcgtaaa tgggggcgca
     6781 ccgagtgtaa agggcgtctg gcagcgaaat acacggcgac gcaactctcg cgtactgaac
     6841 ttggcagtcg ggtaatttgt cgcgataaac aactcatctt tgaagaagcg ccacaagcct
     6901 ataaatcggc tgaaagcgtg gtgcaatgtc tggtgcaggc cggtttaatt attcctgtcg
     6961 cgcggctgcg tccggtgcta acgctcaaaa acagtggagg gaaaaaagga tgatcttgct
     7021 acaactctcc tcagcgcagg ggccggaaga atgttgtctc gcagtgaaaa aggcgctgga
     7081 caggctaatt aaagaagctg cccgacaaga cgtcgcggta acggtgttgg aaacagaaac
     7141 gggccgctac tctgacacgc tgcgttcggc gctgatttct ctggatggcg ataacgcatg
     7201 ggcattaagc gaaagctggt gtggcactat tcagtggatt tgtccgagtc cgtatcggcc
     7261 tcatcatggg cgcaaaaact ggtttctggg cattgggcgt tttaccgctg atgagcagga
     7321 acaatcggat gcaatccgtt atgagacgtt gcgttcgtcg gggccgggcg gtcaacatgt
     7381 caataaaacc gactcggcgg tacgcgccac gcatttggca tccggtatta gcgtgaaggt
     7441 tcagtcagag cgtagtcagc atgctaacaa gcggctggca cgattgctga ttgcctggaa
     7501 gctggagcag cagcaacagg aaaatagcgc ggtgctgaaa tcgcagcggc gaatgttcca
     7561 tcaccagatt gaacgtggca acccgagacg gacatttaca gggatggctt ttatcgaagg
     7621 ataatgaagg atgaaactgc cggaaggcga ttaaacgcca tccggcagca aatcaaataa
     7681 ttacttcgcc ggaatttctt tcagcagttc agtcagcagc gtccagtaat gacctacgct
     7741 ttcgatgtga acttgctcat ccggagagtg tggaccggtg atagttggcc cgatagaaac
     7801 catgtccatt tccggatacg gttttttgaa cagaccacat tccaggcccg cgtggataat
     7861 ctggatgttc ggtgttttgt tgaacagacg ctgataggtt tcacgtacca gatgcatcac
     7921 cggagaatta gcgtccggct gccagccagg atatgcgcct ttcgcttcgg ttttcgcgcc
     7981 agccagttta cccagcgaat ccagcatgct cacgacgtag tctttaccgc tgtcgatcag
     8041 tgaacggatc agacagtgaa tttctacgtt attatcagtc atggtcacca caccgacgtt
     8101 cagggaggtt tcgaccacac cttttgccac gtctgagttg cggatcacac cgttcggggt
     8161 ggcgttcagc agacgaataa aggtatcgcg agatttcgca atcagggcag ctttatcgtt
     8221 cgctacagag tccagcaaca aggccagatt tttctctttc tctgccagct cgtttttcag
     8281 gatctcctga taggtattca ccagagattt caggacgtcg actttatcag ctgcgacagc
     8341 aatggtcgca aaggcttcac gcgggatggc gttacgcagt gtgccgccgt tgaaatcgat
     8401 aaggcgcaga tccagttctt ctgcatgacc cgccaggaag cgcaccagca gtttgttggc
     8461 attacccagg ccaacgtgga tttccccgcc ggagtgaccc cctttcagac cttttaaggt
     8521 taacttgaag gtttcaaaac cagctggaac cgcttcacga tctaaatgca ggttggaggt
     8581 gaagtcgata ccccccgcac aacccatgta gatttcacct tcttcttcgg agtcggtgtt
     8641 aatcagaata tcagcctgca accagttgct ctgtaagccg aacgcaccgt ccataccggc
     8701 ttcttcggtc atggtcagca gcacttccag cgggccgtga accacgtttt cgtcagccag
     8761 aactgccagt gcagaggcca taccaatgcc gttatccgca cccagcgtgg tgccgcgcgc
     8821 tttaacccat tcgccatcaa tataaggctg gataggatct ttcgtgaagt catgcacggt
     8881 gtcgttattt ttctgcggca ccatatcgag gtgggcctgt aagacgaccg gtttacgatt
     8941 ttccatacct gcggtagcag gtttacgaat caggatatta cctacctgat cgcgttcgac
     9001 atggaaacct ttctcttttg cccaaccaac aatgtattca gcgagttgct cttcatgata
     9061 ggacgggtga ggaatagaac agattttggc aaaaatatcc cacagcggct gtggagataa
     9121 ttgagacagt tcagacacgt taagtctcct tgtcgatcac ccgcaaaaca gtattgcagg
     9181 tcacagggtt agcagaaaat gttgtcaaca caagacaggc ttgcgagata tgtttgagaa
     9241 taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa
     9301 tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca
     9361 ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca
     9421 cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac
     9481 aatggaaagg cattattgcc gtaagccgtg gcggtctggt accgggtgcg ttactggcgc
     9541 gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc
     9601 agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg
     9661 atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc
     9721 actttgtcac catctttgca aaaccggctg gtcgtccgct ggttgatgat tatgttgttg
     9781 atatcccgca agatacctgg atcgaacagc cgtgggatat gggcgtcgta ttcgtcccgc
     9841 caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact
     9901 tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca
     9961 aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc
    10021 cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg ctcttgatgg taaaaccatc
    10081 cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggtat tgacccacgt
    10141 gaaatcctag acgtccaggc acgtattgtg atgagcgatg ctgaacgtac cgacgatgat
    10201 ttatacgata ctgtgattgg ctaccgtggt ggcaactgga tttatgagtg ggccacccag
    10261 gcgatggtgt ggcaacaaaa agcctgtgcg gaagaagatc cgcaactcag tggtcgacac
    10321 tggctgcatg cggctacgtt gtacaacatt gccgcctatc ctcatctgaa aggagatgac
    10381 ctggcagagc aagcgcaggc tttatcaaac cgcgcctatg aagaggccgc ccagcgtctg
    10441 ccgggcacga tgcggcagat ggagtttact gtacccggcg gtgcgcccat caccggcttt
    10501 ttgcatatgc cgaaaggcga tggcccgttc ccgacagtat taatgtgtgg tggtctggat
    10561 gcgatgcaga cggactatta cagcctgtat gaacgttatt ttgcgccgcg cggcattgcg
    10621 atgctgacta ttgatatgcc gtcggtgggc ttttcttcaa aatggaagct cacccaggac
    10681 tccagcctgt tgcatcagca cgtcttaaag gcgctgccta acgtaccgtg ggtggatcac
    10741 actcgcgtcg cggcctttgg tttccgtttc ggcgctaacg ttgccgtgcg tctggcatac
    10801 cttgaatcgc cgcgtctgaa agcggttgcc tgtcttggtc cggtagttca taccctgttg
    10861 agtgatttta agtgccagca acaggtgccg gaaatgtatc ttgacgttct ggcgagtcgt
    10921 ttggggatgc atgatgcttc cgacgaagcg ttgcgcgtgg agttgaatcg ctattcatta
    10981 aaagtgcaag gattgctggg acgtcgctgc ccaacgccaa tgttatcagg ctactggaag
    11041 aacgatccgt tcagcccgga agaggactca cgcttaatca cctcatcatc tgctgacggt
    11101 aaattattag agatcccatt taacccggtg tatcggaatt ttgacaaagg tcttcaggaa
    11161 atcaccgact ggatcgaaaa acgtttgtgt taaaaatttg ctaaatttta ccaatttggt
    11221 aaaacagttg catcacaaca ggagattgca atgacgttac cgagtggaca cccgaagagc
    11281 agattgatca aaaaatttac cgcactaggc ccgtatattc gtgaaggtaa gtgcgaagat
    11341 aatcgattct ttttcgattg tctggctgta tgcgtcaacg tgaaaccggc accggaagtg
    11401 cgtgagttct ggggctggtg gatggagctg gaagcgcagg aatcccgttt tacctacagc
    11461 taccagtttg gtctgttcga taaagcaggc gactggaaga gtgttccggt aaaagatact
    11521 gaagtggttg aacgactgga gcacaccctg cgtgagtttc acgagaagct gcgtgaactg
    11581 ctgacgacgc tgaatctgaa gctggaaccg gcggatgatt ttcgtgacga gccggtgaag
    11641 ttaacggcgt gagtgaaatg tgccggatgc atcacatccg gcaatatcta ttaaaactga
    11701 taggtcatgc caaccgcgac aatatcatca ttattaatat tcaatttgtt atcgctatcc
    11761 agttggttga ttttataatc aacaaacgct gacatatttt tgttgaaata atatgtagcc
    11821 ccgacatcga tataattgac cagatcttca tcaccgatac cttcaatatc tttccctttc
    11881 gataagacat aacccagcga tggacgcaga ccaaagtcaa actggtattg agcgaccgct
    11941 tcaaagttct gtgtcttatt ggcaaagccg ccagttattg gcgtcatttt gcgtgtttca
    12001 gaataaaaag ttgccagata aatattattg gcatcgtatt tcagacctgt agcccatgct
    12061 tctgcacgct tgcctgtgcc acggctttgc aggttctgct cgttggtgcg atctgagttg
    12121 gtataggccc cactaatggc gaaatcgctg ccgccaaagt catatgtcaa tgacgtgccg
    12181 aagccatcgc cgttttgctt tttaacgtcg cggttttcgt ttttcccttg atattgcagg
    12241 gttaagttca ggccatcgat aacgccgaag aagtcggtgt tccgatacgt cgccagaccg
    12301 ctggcgcgtt tggtcataaa gttgtcggtc tgcgccgagg agtcgccacc aaattccggg
    12361 aacatatcgg tccaggcttc cacgtcatac aacgcgccca ggttacggcc atagtcgaaa
    12421 gaacccaaat ctttatactt caatccggca aaagcgagac gcgttttttg ctgtgcagta
    12481 tcactctccg ctttatttcc ggcaaactcc gcttcccaac ggccatagcc agtcagttga
    12541 tcgttaattt gtgtttcgcc tttaaaacca aaacggatat aactctggtc gccatcttta
    12601 ctgtcgttat cactcatata atgcatggct ttaactttgc catagacatc cagtttatta
    12661 ccgtctttgt tatatatttc tgcggcctgt acggatgcag atgccacaat gcccatcacc
    12721 actaatgcca gagtgctctt tttcattttc attcctgatt ttaattaacg cgcgaatatt
    12781 cagcgggaga gccccgttga aaacaggaaa gtttttaacc tgagattgtt aaagatatat
    12841 tacagattaa taatattctt aaaatgtggt aatttattaa atctgtaata aaagcgtaaa
    12901 caactgccgc taggcttgct gatcccgcgc aacaaaacgc catgctttgc ttgcagatgg
    12961 ttggcaaccg acgacagtcc tgctaaaacg ttcgtttgat atcatttttc ctaaaattga
    13021 atggcagaga atcatgagtg acagccagac gctggtggta aaactcggca ccagtgtgct
    13081 aacaggcgga tcgcgccgtc tgaaccgtgc ccatatcgtt gaacttgttc gccagtgcgc
    13141 gcagttacat gccgccgggc atcggattgt tattgtgacg tcgggcgcga tcgccgccgg
    13201 acgtgagcac ctggggtacc cggaactgcc agcgactatc gcctcgaaac aactgctggc
    13261 ggcggtaggg cagagtcgac tgattcaact gtgggaacag ctgttttcga tttatggcat
    13321 tcacgtcggg cagatgctgc tgacccgtgc tgatatggaa gaccgtgaac gcttcctgaa
    13381 cgcccgcgac accctgcgtg cgttgctcga taacaatatc gttccggtaa tcaatgagaa
    13441 cgatgctgtc gctacggcag agattaaggt cggcgataac gataaccttt ctgcgctggc
    13501 ggcgattctg gcgggtgccg ataaactgtt gctgctgacc gatcaaaaag gtttgtatac
    13561 cgctgatccg cgcagcaatc cgcaggcaga actgattaaa gatgtttacg gcattgatga
    13621 cgcactgcgc gcgattgccg gtgacagcgt ttcaggcctc ggaactggcg gcatgagtac
    13681 caaattgcag gccgctgacg tggcttgccg tgcgggtatc gacaccatta ttgccgcggg
    13741 cagcaagccg ggcgttattg gtgatgtgat ggaaggcatt tccgtcggta cgctgttcca
    13801 tgcccaggcg actccgcttg aaaaccgtaa acgctggatt ttcggtgcgc cgccggcggg
    13861 tgaaatcacg gtagatgaag gggcaactgc cgccattctt gaacgcggca gctccctgtt
    13921 gccgaaaggc attaaaagcg tgactggcaa tttctcgcgt ggtgaagtca tccgcatttg
    13981 caacctcgaa ggtcgcgata tcgcccacgg cgtcagtcgt tacaacagcg atgcattacg
    14041 ccgtattgcc gggcaccact cgcaagaaat tgatgcaata ctgggatatg aatacggccc
    14101 ggttgccgtt caccgtgatg acatgattac ccgttaagga gcaggctgat gctggaacaa
    14161 atgggcattg ccgcgaagca agcctcgtat aaattagcgc aactctccag ccgcgaaaaa
    14221 aatcgcgtgc tggaaaaaat cgccgatgaa ctggaagcac aaagcgaaat catcctcaac
    14281 gctaacgccc aggatgttgc tgacgcgcgt gccaatggcc ttggcgaagc gatgcttgac
    14341 cgtctggcac tgacgcccgc acggctgaaa ggcattgccg atgatgtgcg ccaggtgtgt
    14401 aacctcgccg atccggtggg gcaggtaatc gatggcagcg tactggacag cggcctgcgt
    14461 cttgagcgtc gtcgcgtgcc gctgggggtg attggcgtga tttatgaagc gcgcccgaac
    14521 gtgacggttg atgtcgcttc gctgtgcctg aaaaccggta atgcggtgat cctgcgtggt
    14581 ggcaaagaaa cctgtcgcac taacgcggca acggtggcgg tgattcagga cgccctgaaa
    14641 tcctgtggct taccggcggg tgccgtgcag gcgattgata atcctgaccg tgcgctggtc
    14701 agtgaaatgc tgcgtatgga taaatacatc gacatgctga tcccgcgcgg cggggctggt
    14761 ttgcataaac tgtgccgcga acagtcgaca atcccggtga tcacaggtgg tataggcgta
    14821 tgccatattt acgttgatga aagcgcagag atcgctgaag cattaaaagt gatcgtcaac
    14881 gcgaaaactc agcgaccgag cacatgtaat acggtagaaa cgttgctggt gaataaaaac
    14941 atcgccgata gcttcctgcc cgcattaagc aaacaaatgg cggaaagcgg cgtgacatta
    15001 cacgcagatg cagctgcgct ggcgcagttg cagacaggcc ctgcgaaggt ggtggcggtt
    15061 aaagccgaag agtatgacga tgaatttctg tcattagatt tgaacgttaa aatcgtcagc
    15121 gatcttgacg atgccatcgc ccatattcgt gaacacggca cacaacactc cgatgcgatc
    15181 ctgacccgcg atatgcgcaa cgcccagcgt tttgttaacg aagtggattc gtccgctgtt
    15241 tacgttaacg cctctacgcg ttttaccgac ggcggccagt ttggtctggg tgcggaagtg
    15301 gcggtaagca cacaaaaact ccacgcgcgt ggcccaatgg ggctggaagc actgaccact
    15361 tacaagtgga tcggcattgg tgattacacc attcgtgcgt aaataaaacc gggtgatgca
    15421 aaagtagcca tttgattcac aaggccattg acgcatcgcc cggttagttt taaccttgtc
    15481 caccgtgatt cacgttcgtg aacatgtcct ttcagggccg atatagctca gttggtagag
    15541 cagcgcattc gtaatgcgaa ggtcgtaggt tcgactccta ttatcggcac catttcaaac
    15601 tcttcccaag tctaccgaaa tcaactaaaa gctcgtatac tgcggtttct ggcccgtatc
    15661 ttatctcctg ttatcaacta gactcaaccg aaatcaagtc gcagttgggg gcataagtgg
    15721 gggcattttg tgttcggtct agggagatgc ccccaatgaa acttaatgcg cgacaggtag
    15781 acgcagctaa acccagagag aaagcctata agctggcaga tggtgctggt ttgtatcttg
    15841 aggttgttcc ctctggttcc agatactggc ggatgaaata tcgcttcaat ggaaaagaga
    15901 agcgtatggc ctttggtgtc tatccggcag tgtcccttgc acaagcgagg gcactgcgtg
    15961 acgacgccaa gaaaaagctt gccgaaggta tcgatccatc gcttgccaag aaagaagaaa
    16021 agctggttcg agatgtgcag ctaaataata cgtttcaggc tgtggcactt gaatggcatg
    16081 gaacgaaggt gagccgatgg tcagaaggtt atgcctcgga cattatcgaa gcctttaata
    16141 aagatatttt tccctatatt ggccaacaac cggtgaatga aatcaaacag ctggttctgc
    16201 ttaatgtgct gcgtcgaatt gaaagccgtg gcgcgacaga gaaggccaag aaggttcgcc
    16261 agcgttgcag cgaagtcttt cgttacgcca tcgtaaccgg tcgtgcggaa tacaatcctg
    16321 cagcggatct taccagcgcg atgtcagggc atgaatcgaa gcattatccc ttccttactg
    16381 ttgaggagtt accagacttt tttaaagctc tcgcaggcta cacaggaagc ccgttagttg
    16441 ttcttgcggc acgtctgctg atcctcacgg gagttcgcac tggcgaactc cgaggtgctt
    16501 tctggagtga gtttgatctt gaaaaagcgg tgtgggagat acctgccgag cgtatgaaga
    16561 tgaaacggcc tcaccttgtc cccctctctt cccaagcgct ggaaatcgta cagcaactca
    16621 aagtgatgtc agggcaatat ccactggtgt tcccgggacg taatgatccc cgcaagacga
    16681 tgagtgaagc gagtattaat caggttttta agcggattgg atatacgggg agggtaacgg
    16741 ggcatggttt ccgtcacacg atgagtacga ttttgcatga ggaaggtttc aatacagcgt
    16801 ggattgaaac ccagctggct cacgttgata aaaatgcgat tcgtgggacg tataaccatg
    16861 cgttgtattt ggaaggacgt agggagatga tgcagtggta tgcggactat ataaacgcta
    16921 atcataattc atgtatttct ttgctggtga acgctacaaa gtagatttga cgtgatgata
    16981 ggattgatta ggaaaatgac ctaaaaccgt atattttgat acttaagttt aaacattagc
    17041 cctatatccg atcgcccttt gttacctaca cttctatctt ttttaggttc tagggctgaa
    17101 agatttgaag ataagtttat aaatatcaat agattacatg attgtgttgg ttcttgggta
    17161 gccgtaacgc ctagggcggt agcgtactta agatgagtat aaaaaatgaa cagtaactat
    17221 atgccattca cacaacgaga tagtctcgga agatactata caaaagaatc cataagtgca
    17281 ttattggttt ctcagatgaa agctgaaaaa gtcaataata ttattgattt agcttctggt
    17341 gaggggagct taacctatgc tgctttagac cgatggaaga atgcagaagc atattctttg
    17401 gatatagaat ctcggatgtc aaaaaaagta tgtgataatc ttactcatat tgtaacagat
    17461 gcccttgttc attcatttcc tgaaatgctt gcccgacatc aaggaaattt tgatgttgca
    17521 gtatgtaatc ctccattcac tctccctgaa tggagggatg attattttaa aatcattagt
    17581 gagattggcg cagataaata tatatctgtc tcaaaatatg tcccagcaga gataatattt
    17641 atatctcaag tcatcaggtt tcttaaaaag ggcggtgagg ctggaataat tttacctgat
    17701 ggtatattta cagcaagaaa gtttataggt ttaagacgat atttattgaa tgaacattca
    17761 attacaaaag tcattgaatt gcctaggaat atcttcaaaa ggacagaggc taaaacacat
    17821 attttaattt ttaataaaaa aattatgcct catcataaaa tacaattaca ttgtataact
    17881 aaagatgggg aattgtcgcc tcctgtttta attagaaaag aagatgcggt tgagagaatg
    17941 gactactctt atcattataa taaaaatgaa ggtaaagggt ttagcacaat agggatgctt
    18001 aaaaatattt caatttttag aggtaggttt aattcaaagg aaattacgga acatgttttt
    18061 catacgacaa aatttagtgg tgatgaaaag tacattaaat tccactgcaa ctctgtagaa
    18121 gaattgaagc catcaaaatt agatgtcatt gctaagcctg gtgatatatt aatagcaaga
    18181 gttgggcgaa attttcataa aaaaatattg tttgttgaga gcggctattc ttatatcagt
    18241 gactgtattt ttctgatacg agcctctggt ggagataaga aaaaactatt tgattttctt
    18301 tgttctcaag atgggcaaga ggaattatct cgagcgagta gtggtgtagc cgcacaacat
    18361 attacaatgg atgcattaaa aaaaatacat cttgtaagga ttaaacatga ctgatgtgga
    18421 attagatagt aattttctta tcgaggaacg agctgattat attgatgagg atacgataaa
    18481 aaataatact at
//
LOCUS       DAZGSP010000099        18248 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZGSP010000099 DAZGSP010000000
VERSION     DAZGSP010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.100"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            283..966
                     /locus_tag="ACHK74_003850"
     CDS             283..966
                     /locus_tag="ACHK74_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP0222588.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1010..5860
                     /gene="ehaG"
                     /locus_tag="ACHK74_003851"
     CDS             1010..5860
                     /gene="ehaG"
                     /locus_tag="ACHK74_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFP0222589.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIRETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVATEENTISVGSS
                     TNQRRITNVAAGKNDTDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6228..7883
                     /gene="lldP"
                     /locus_tag="ACHK74_003852"
     CDS             6228..7883
                     /gene="lldP"
                     /locus_tag="ACHK74_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFP0222590.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7883..8659
                     /gene="lldR"
                     /locus_tag="ACHK74_003853"
     CDS             7883..8659
                     /gene="lldR"
                     /locus_tag="ACHK74_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFP0222591.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHYEHSREKNA"
     gene            8656..9846
                     /gene="lldD"
                     /locus_tag="ACHK74_003854"
     CDS             8656..9846
                     /gene="lldD"
                     /locus_tag="ACHK74_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFP0222592.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10032..10505
                     /gene="trmL"
                     /locus_tag="ACHK74_003855"
     CDS             10032..10505
                     /gene="trmL"
                     /locus_tag="ACHK74_003855"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFP0222593.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10558..11379)
                     /gene="cysE"
                     /locus_tag="ACHK74_003856"
     CDS             complement(10558..11379)
                     /gene="cysE"
                     /locus_tag="ACHK74_003856"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFP0222594.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11459..12478)
                     /gene="gpsA"
                     /locus_tag="ACHK74_003857"
     CDS             complement(11459..12478)
                     /gene="gpsA"
                     /locus_tag="ACHK74_003857"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP0222595.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12478..12945)
                     /gene="secB"
                     /locus_tag="ACHK74_003858"
     CDS             complement(12478..12945)
                     /gene="secB"
                     /locus_tag="ACHK74_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFP0222596.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(13008..13259)
                     /gene="grxC"
                     /locus_tag="ACHK74_003859"
     CDS             complement(13008..13259)
                     /gene="grxC"
                     /locus_tag="ACHK74_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFP0222597.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13401..13832)
                     /locus_tag="ACHK74_003860"
     CDS             complement(13401..13832)
                     /locus_tag="ACHK74_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP0222598.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVIDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14077..15621
                     /gene="gpmM"
                     /locus_tag="ACHK74_003861"
                     /gene_synonym="pgmI"
     CDS             14077..15621
                     /gene="gpmM"
                     /locus_tag="ACHK74_003861"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFP0222599.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15655..16914
                     /gene="envC"
                     /locus_tag="ACHK74_003862"
     CDS             15655..16914
                     /gene="envC"
                     /locus_tag="ACHK74_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFP0222600.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16918..17877
                     /locus_tag="ACHK74_003863"
     CDS             16918..17877
                     /locus_tag="ACHK74_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFP0222601.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17864..>18248)
                     /gene="waaH"
                     /locus_tag="ACHK74_003864"
     CDS             complement(17864..>18248)
                     /gene="waaH"
                     /locus_tag="ACHK74_003864"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFP0222602.1"
                     /translation="HNTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYP
                     EFHQQITYEALRVCHAVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLW
                     SFRLWQWRDKTRSHHRITRSAFNLR"
BASE COUNT         4602 a         4703 c         4734 g         4209 t
ORIGIN      
        1 tcatattcac aaaatcaatt atctgtaaaa tttttcccag acatggtttc tttcatattt
       61 gacatttatt aataatcatc attagaatcc acgaaaaata atatattcat acaaacacag
      121 acatcttttg aaacgaatga tacattttta tgatgaatgc ttaaaacaat atttaatcga
      181 cacacatcac tgtcattaat gtaaagcaac atagctcacc aataatacaa tggacaactc
      241 taccggcatt gatcgctggt cggaggaaat aaggattttt acatgaactt gaagaagatt
      301 tttttcagtg ctgtcactgt ttcagttctc tgcgcattaa cgggatgtga ctacattgaa
      361 gaaggcaagc cagaaagtag tttactgaaa caacaagaag aacataataa taaaattgtg
      421 ctacttgaaa aacaacaagc acagctaaag agtcaactcg aaactattca aaaacaacaa
      481 actggcataa taaacagtac aaagacttta actcacgtaa ttaaatccgt taaagaccaa
      541 caaaatactt ttatttttac cgagtttaac ccagcaaaaa cgaagtattt catcctgaac
      601 aacggttcgg tggctttagc gggtcgagtg ttatccattg acgccacaga gaatggtagt
      661 gttattcata tctcactggt caacttatta agtacaccta tctcaaacat tggttttaat
      721 gcgacatggg gtggtgaaaa acctgtcgat gccaaagagt ttgcccggtg gcaacaatta
      781 cttttcaaca catcaatgaa atccacattg aaattattac caggtcaatg gcaagacatt
      841 aatttgaccc tgaagggtgt atcgcccaat aacctgggat atctgaagtt agccatcaac
      901 atggaaaata ttcagttcga caatcttccc tctgctgaaa atcggcagaa aagaagcaaa
      961 aaataaatgc agttagtacg cttataacaa ggaatagcga gcatcattaa tgaacaaaat
     1021 atttaaagtt atctggaacc ctgcgacagg gaattatact gttaccagcg aaacggcaaa
     1081 aagccgtggc aagaaatctg ggcgcagtaa gctgttaatt tctgcgctgg ttgcgggtgg
     1141 aatgttgtcg tcgtttgggg cattggcgaa tgccgggaat gacaacggtc agggtgttga
     1201 ttacggtagt ggatcagctg gcgacggctg ggttgctata ggcaaagggg cgaaagcaaa
     1261 tacttttatg aacaccagtg gttccagtac tgctgtgggt tatgacgcta tagctgaagg
     1321 ccaatatagc tctgccatcg ggtcaaaaac ccatgcgatt ggcggtgcat caatggcctt
     1381 tggggttagt gcaatatcag aaggcgatag aagtatagca ctgggtgcct cttcgtattc
     1441 attgggccaa tactcaatgg ccctcggccg ttattcaaaa gcattgggta aattgtctat
     1501 tgctatgggg gactcttcca aagcggaagg agcaaacgcc attgccctgg gaaatgccac
     1561 taaagctact gagattatga gtattgctct tggcgacacc gccaatgcgt caaaagcgta
     1621 ttcaatggcg ctgggagcaa gtagcgtcgc atctgaagaa aacgcaattg ccctggggcg
     1681 tagcagtgta gctagcggta ctgacagcct cgcatttggc agacaatcac ttgccagcgc
     1741 agcgaacgct attgcgatag gtgctgagac cgaagccgct gaaaatgcaa ctgctattgg
     1801 caataatgcg aaggcaaaag ggactaatag catggcaatg gggttcggaa gccttgccga
     1861 taaagtcaat actatcgcat taggaaatgg cagccaggct ctggcagata atgcaatcgc
     1921 cataggccag ggcaacaaag ctgatggcgt ggatgccatc gctctgggta atggtagcca
     1981 gtcgagaggc ttaaacacca ttgccttagg cacagccagt aatgcaactg gtgataagag
     2041 tcttgcgctt ggtagtaata gcagtgccaa cggtattaac tctgtcgcgc tgggcgcaga
     2101 ttccattgcg gatttagaca ataccgtctc tgtcggcaat agttcattaa aacgcaagat
     2161 cgttaatgtg aaaaatggcg cgatcaagtc tgacagttac gatgccatta atggttcaca
     2221 gctttatgcc attagcgact cggtagcaaa aaggcttgga ggaggggctg cagtagatgt
     2281 tgatgacggt actgttacag caccaaccta caatttaaaa aatggtagca aaaataacgt
     2341 aggggctgcg ctcgctgtac ttgatgaaaa caccctgcaa tgggaccaaa ccaaaggcaa
     2401 atacagcgct gctcatggta ctagtagccc aactgccagc gtaatcaccg atgttgcgga
     2461 tggcacgatt tcagcctcca gtaaggatgc ggttaacggt tcccaactga aagctaccaa
     2521 tgacgatgtc gaagccaaca ccgccaatat cgctactaat accagcaaca ttgccacgaa
     2581 tacggcaaat attgccacca ataccaccaa tatcaccaac ctgacggatt ccgttggtga
     2641 ccttcaggct gatgccctgc tctggaacga aactaaaaag gcattcagtg cagctcacgg
     2701 ccaggatacc accagcaaaa tcaccaacgt taaagatgcc gacctgacgg ctgacagcac
     2761 tgatgctgtt aacggctctc agctgaaaac caccaacgat gctgtggcga cgaataccac
     2821 caatatcgcc aataacactt ccaatattgc cactaacacc accaacatct ctaacctgac
     2881 tgagacggtg actaatcttg gtgaggatgc gctgaaatgg gataaggaca atggtgtatt
     2941 cacggcagct catggcaccg agaccaccag caaaatcacc aacgttaaag atggcgacct
     3001 gacgactggc agcaccgatg ccgttaacgg ctctcagctg aaaaccacca acgatgccgt
     3061 ggcgacgaat accaccaata tcgccactaa caccaccaac atctctaatc tgactgagac
     3121 ggtgactaat cttggtgagg atgcgctgaa atgggataag gacaatggtg tcttcactgc
     3181 agctcatggc aacaataccg ccagcaaaat caccaatatc ctggacggca cagtcactgc
     3241 aaccagttcc gatgccatta acggtagcca gctttatgac ttaagcagca atatcgccac
     3301 ctacttcggc ggcaatgctt ctgtgaatac tgacggtgtg tttaccggtc caacctacaa
     3361 aatccgtgaa acaaattatt ataacgtcgg cgatgcactg gctgcgatta actcctcatt
     3421 tagcacgtct ctcggcgatg ctctgctttg ggatgccacc gcaggtaaat tcagtgccaa
     3481 acacggtact aatggtgacg caagcgtgat cactgatgtc gcagatggtg aaatttcaga
     3541 ctccagttct gacgcagtaa acggctcaca actccacggc gtgagcagtt atgttgttga
     3601 tgcgctgggg ggtggtgccg aagtcaatgc agacggcacc atcactgcgc cgacgtacac
     3661 cattgctaat gctgattacg ataatgtcgg tgatgccctg aatgctatcg ataccactct
     3721 tgacgacgct ctgctctggg atgcggacgc cggtgaaaat ggtgcattta gcgccgctca
     3781 cggaaaagat aaaactgcca gtgtaatcac taacgtcgct aacggtgcaa tctctgctgc
     3841 cagcagcgac gcgattaacg gctcacaact ctataccacc aataagtaca tcgctgatgc
     3901 gctgggtggt gacgcagaag tcaacgctga cggcaccatc accgcaccga cttacaccat
     3961 tgcgaacgcc gagtacaaca acgtcggtga cgccctggat gcgcttgatg ataacgccct
     4021 gctgtgggat gagactgcca atggcggtgc tggagcctac aatgccagcc atgacggtaa
     4081 agccagcatc atcactaatg tcgctaatgg cagtattagt gaggacagta ccgatgcagt
     4141 gaacggttct cagttgaatg cgacgaatat gatgattgag cagaacaccc aaattatcaa
     4201 tcagctcgct ggtaacaccg acgcaaccta tatccaagaa aacggtgcgg gtattaacta
     4261 tgtgcgtact aacgacgacg gcttagcgtt caacgacgcc agcgcacagg gtgttggcgc
     4321 tacagctata ggttataact ctgtcgccaa aggcgatagc agcgtagcta ttggtcaggg
     4381 cagctacagc gacgttgata cgggtatcgc cctgggtagc agctctgttt ccagccgagt
     4441 gattgccaaa ggctcccgtg acaccagcat aacggaaaat ggcgttgtta ttggttacga
     4501 caccacggat ggcgaactgc tcggtgcatt gtctatcggt gatgacggta aatatcgtca
     4561 aatcatcaac gtagccgatg gttccgaagc ccatgacgcc gttacggttc gtcaattgca
     4621 gaatgcgatt ggtgcggtcg caaccacgcc gactaaatac ttccacgcta attcaacgga
     4681 agaagattca ctggcagtgg gaactgactc gctggcaatg ggtgcgaaaa ccatcgtgaa
     4741 tggcgataaa ggtattggta tcggttatgg tgcctacgtg gacgcgaatg cacttaacgg
     4801 cattgccatt ggtagcaatg cgcaagtcat tcatgtcaac agtattgcga taggtaatgg
     4861 ttctacgacc actcgtggcg ctcaaaccaa ttataccgcc tacaacatgg acgcaccgca
     4921 gaactctgtc ggtgaattct cagtcggtag tgcggatggt caacgtcaga tcactaacgt
     4981 cgcagcaggt tcggctgata ccgatgcggt caacgtgggt cagttgaaag taacggatgc
     5041 gcaggtttcc cagaataccc agagcattac taacctggat aatcgggtaa cgaatcttga
     5101 ttcacgcgtc accaatatcg aaaacggtat tggcgatatc gtcaccaccg gtagcaccaa
     5161 gtacttcaag accaataccg atggtgtaga tgccagcgcg cagggtaaag atagcgtcgc
     5221 gattggttct ggctccattg ctgccgcgga taacagcgtc gcactgggta cagggtctgt
     5281 ggcaaccgaa gaaaatacga tctctgtagg ctcatctact aatcaacgtc gtatcaccaa
     5341 cgtagccgca ggtaaaaacg acaccgatgc tgttaacgtg gcacagttga agtcttccga
     5401 agctggcggt gtgcgttacg acaccaaagc tgatggttct atcgactata gcaatatcac
     5461 cctcggtggc ggcaacggcg gtacgactcg tatcagcaac gtctccgctg gcgtcaacaa
     5521 caacgacgcg gtgaattacg cgcagttgaa gcaaagcgtg caggaaacga agcaatacac
     5581 cgatcagcga atggttgaga tggataacaa actgtctaaa actgaaagca agttgagcgg
     5641 tggtatcgct tctgcaatgg caatgaccgg tctgccgcag gcttatacac cgggtgccag
     5701 catggcttct attggtggcg gtacttacaa cggtgaatcg gcagttgctt taggtgtatc
     5761 gatggtgagc gccaatggtc gttgggtcta caaattacaa ggtagtacca atagccaggg
     5821 tgaatactcc gccgcactcg gtgccggtat tcagtggtaa tgatccatta agttagtgtg
     5881 actaagggca aacgccaaat tgcctgatgc gttacgcgca tcaggcaaga taaggcgctc
     5941 cgggtcaaca atctaagggc aattctctga tgaggattgc ccttttcttt accagacatc
     6001 tccccccaca agaattggcc ctaccaattc ttcgcttatc tgacctctgg ttcacaattc
     6061 cccaattaaa actcacatca atattgccaa tacataacat ttagttaacc attcattgtc
     6121 attatcccta cacaacacaa ttggcagtgc cacttttaca caacgtgtga caaggagatg
     6181 agcaacagac tcattacacg atgtgcgtgg actccaggag acctgcaatg aatctctggc
     6241 aacaaaacta cgatcccgcc gggaatatct ggctttccag tctgatagca tcgcttccca
     6301 tcctgttttt cttctttgcg ctgattaagc tcaaactgaa aggatacgtc gccgcctcat
     6361 ggacggtagc aatcgccctt gccgtggctt tgctgttcta taaaatgccg gtcgctaacg
     6421 cgctggcctc ggtggtttat ggcttcttct atggtttgtg gcccatcgcg tggatcatta
     6481 ttgcagcagt gttcgtctat aagatctcgg tgaaaaccgg gcagtttgac atcattcgct
     6541 cgtctattct ttcgataacc cctgaccagc gtctgcaaat gctgatcgtc ggtttctgtt
     6601 tcggcgcttt ccttgaagga gccgcaggct ttggcgcacc ggtagcaatt accgccgcat
     6661 tgctggtcgg cctgggtttt aaaccgctgt acgccgccgg actgtgcctg attgttaaca
     6721 ccgcgccagt ggcatttggc gcgatgggca ttccaatcct ggttgccgga caagtaacag
     6781 gcatcgacag ctttgagatt ggccagatgg tggggcggca gctaccgttt atgaccatta
     6841 tcgtgctgtt ctggatcatg gcgattatgg acggctggcg cggtatcaag gagacgtggc
     6901 ctgcggtcgt ggttgcgggc ggctcgtttg ccatcgctca gtaccttagc tctaacttca
     6961 ttgggccgga gctgccggac attatctctt cgctggtatc actgctctgc ctgacgctgt
     7021 tcctcaaacg ctggcagcca gtgcgcgtat tccgttttgg tgatttgggg gcgtcacagg
     7081 ttgatatgac gctggcccac accggttaca ctgcgggtca ggtgttacgt gcctggacac
     7141 cgttcctgtt cctgacagct accgtaacac tgtggagtat cccgccgttt aaagccctgt
     7201 tcgcatcggg tggtgcgctg tatgagtggg tgatcaacat tccggtgccg tacctcgata
     7261 aactggttgc ccgtatgcca ccagtggtca gcgaggctac agcctatgcc gccgtgttta
     7321 agtttgactg gttctctgcc accggcaccg ccattctgtt tgctgcactg ctctcgattg
     7381 tctggctgaa gatgaaaccg tctgacgcta tcagcacctt cggcagcacg ctgaaagaac
     7441 tggctctacc catctactcc atcggcatgg tgctggcgtt cgcctttatc tcgaactatt
     7501 ccggactgtc atcaacactg gcactggcac tggcgcacac cggtcatgca ttcaccttct
     7561 tctcgccgtt cctcggctgg ctgggggtat tcctgaccgg gtcggatacc tcatctaacg
     7621 ccctgttcgc cgcgctgcaa gccaccgcag cacaacaaat tggcgtctct gatctgttgc
     7681 tggttgccgc caataccacc ggtggcgtca ccggtaagat gatctccccg caatctatcg
     7741 ctatcgcctg tgcggcggta ggcctggtgg gcaaagagtc tgatttgttc cgctttactg
     7801 tcaaacacag cctgatcttc acctgtatgg tgggcgtgat caccacgctt caggcttatg
     7861 tcttaacgtg gatgattcct taatgattgt tttacccaga cgcctgtcag acgaggttgc
     7921 cgatcgtgtg cgggcgctga ttgatgaaaa aaacctggaa gcgggcatga agttgcccgc
     7981 tgagcgccaa ctggcgatgc aactcggcgt atcacgtaat tcactacgcg aggcgctggc
     8041 aaaactggtg agcgaaggcg tgctgctcag tcgacgcggc ggcgggacgt ttattcgctg
     8101 gcgtcatgac acatggtcgg agcaaaacat cgtccagccg ctaaaaacgc tgatggccga
     8161 tgatccggat tacagtttcg atattctgga agcccgctac gccattgaag ccagcaccgc
     8221 atggcatgcg gcaatgcgcg ccacacctgg cgacaaagaa aagattcagc tttgctttga
     8281 agcaacgcta agtgaagacc cggatatcgc ctcacaagcg gacgttcgtt ttcatctggc
     8341 gattgccgaa gcctcacata acatcgtgct gctgcaaacc atgcgcggtt tcttcgatgt
     8401 cctgcaatcc tcagtgaagc atagccgtca gcggatgtat ctggtgccac cggttttttc
     8461 acaactgacc gaacaacatc aggctgtcat tgacgccatt tttgctggtg atgccgacgg
     8521 ggcgcgtaaa gcaatgatgg cgcaccttag ttttgttcac accaccatga aacgattcga
     8581 tgaagatcag gctcgccacg cacggattac ccgcctgccc ggtgagcatt atgagcattc
     8641 gagggagaaa aacgcatgat tatttccgca gccagcgatt atcgcgccgc agcgcaacgc
     8701 attctgccgc cgttcctgtt ccactatatg gatggtggtg catattctga atacacgctg
     8761 cgccgcaacg tggaagattt gtcagaagtg gcactgcgcc agcgtattct gaaaaacatg
     8821 tctgacttaa gcctggaaac gacgctgttt aatgagaaat tgtcgatgcc ggtagcactg
     8881 gcaccggtgg gtttgtgtgg catgtatgcg cgtcgtggcg aagttcaggc agccaaagcg
     8941 gcggacgcgc atggcattcc gtttactctc tcgacggttt ccgtttgccc gattgaagaa
     9001 gtcgcgccag ccatcaagcg cccaatgtgg ttccagcttt atgtactgcg cgatcgcggc
     9061 tttatgcgta acgcgctgga gcgagcaaaa gcagcgggtt gttcgacgct ggttttcacc
     9121 gtggatatgc cgacaccggg cgcacgctac cgtgatgcgc attcaggtat gagcggccca
     9181 aatgcggcaa tgcgccgcta cttgcaggcg gtgacgcatc cgcaatgggc gtgggatgtg
     9241 ggcctgaacg gtcgtccaca tgatttaggt aatatctcag cttatctcgg caaaccgacc
     9301 ggactggaag attacatcgg ctggctgggg aataacttcg atccgtccat ctcatggaaa
     9361 gaccttgagt ggatccgcga tttctgggat ggcccgatgg tgatcaaagg gatcctcgat
     9421 ccggaagatg cgcgcgatgc agtacgtttt ggtgctgatg gaattgtggt ttctaaccac
     9481 ggtggccgcc agctggacgg tgtactctct tccgctcgtg cgttgcccgc tattgcagat
     9541 gcggtgaaag gtgatatcgc cattctggcg gatagcggaa ttcgtaacgg gcttgatgtc
     9601 gtgcgtatga ttgcgctcgg tgccgacacc gtacttctgg gtcgtgcttt cctgtatgcg
     9661 ctggcaacag cgggccaggc gggtgtagct aacctgctaa atctgatcga gaaagagatg
     9721 aaagtggcaa tgacgctgac tggcgcgaaa tcgatcagtg aaattacgca agattcgctg
     9781 gtgcaggggc tgggtaaaga gttgcctacg gcactggctc cgatggcgaa agggaatgcg
     9841 gcatagtcgt ttgagccccc tcaccctaac cctctccctc agggagtggg gaccgttcgg
     9901 cgctgtatgt actccctcac tctgaaacga caccgcactc tttttttctc cctcgcccct
     9961 ccggggtgag gggaaaaggc cgcactgctc accaattttc tgctatcctg cccccgcact
    10021 aagggggcag tatgctaaac atcgtacttt acgaaccaga aattccgcca aatactggca
    10081 atatcatccg tctttgcgct aataccggct ttcgtctgca tatcatcgaa ccgatgggat
    10141 ttgcctggga cgataagcgc ctgcgccgcg cggggctgga ctatcacgag tttaccgccg
    10201 ttacgcgtca tcatgacttt cgcgcattcc tcgaagcaga aaatccccag cgcctgtttg
    10261 ccctcaccac gaaaggtacg cctgctcaca gcgccgtaaa ctatcaggat ggcgattatc
    10321 tgatgttcgg cccggaaaca cgcggcctgc cagcgagcat tcttgatgcc ctgcccgctg
    10381 aacaaaaaat tcgcattccg atggtgccgg acagccgcag catgaacctg tccaatgcgg
    10441 tgtcagttgt ggtgtatgaa gcctggaggc agctgggata tccgggagcg ttgttgagag
    10501 attagttact gtatgccgga tagatgatta catcgcatcc ggcacgatca caggacatca
    10561 gatcccatcc ccatactcaa atgtatggtt aataccgttg aaatgctggt ccatatccat
    10621 tgatggctta tcgctgtctg gtttaccgac aatacgagcc ggaacgccag cggcggttgt
    10681 atgcggcggc accggttgca gcaccacgga acctgcgcca atcttcgcgc cgcgaccaac
    10741 ttcaatattg ccgaggattt tcgcgcccgc gccaatcatc acaccttcac gaattttcgg
    10801 gtgacggtca ccaccagatt tacccgtacc gccaagcgtc acagattgca gaatcgatac
    10861 gtcgttttca attaccgccg tttcacccac aacgatgccc gtggcgtggt cgagcataat
    10921 gccacggcca atttttgctg ccgggtgaat atcgacctgg aacgtcacag aaacctggtt
    10981 ttgcagaaag attgccaatg cgcgacgccc ctgattccac aaccagtgac cgatgcgata
    11041 ggcctgcaag gcatgaaaac ccttcaggta taacaacggg gttgagtatt tatcgactgc
    11101 cgggtcgcgg gtacgcaccg cctgaatatc acaggccgca gaggcgatca tttccgggtc
    11161 agcggcgtag gcttcttcca ccacttcacg gatagcaata gcaggcataa ttggcgatga
    11221 cagcttgttc gccagcatgt agctcagtgc actaccaagg ttttcgtgct tgagtagcgt
    11281 cgcgtggtaa aaactggcca gcattggctc acagtccgcc agcgttctgg cttcggcttt
    11341 aatattgttc cagacaattt ccagttcttc acacgacatt gcttactcca cacgatgaga
    11401 taatgaccgg cccgttctgc gcgggccggg tcatagcggt aacaaaggtt ccctggggtt
    11461 agtggctgct gcgctcgtcc ttgcgtgcac gacctaataa cgtcaatgct gcctcgcgcg
    11521 cgttttttcc gcaatataat acttgataaa tttcctcggt tattggcatt tcaacgccga
    11581 agcgatgcgc cagttcgcgg acttctttcg tattgcggta gccttccacc acctgaccaa
    11641 tcttctcctg cgcgctttgt acatccatgc cctgaccgag catcatgcca aaacggcggt
    11701 tacgcgactg gttgtcggta caggtaagca ccagatcgcc aagccccgcc atgcccataa
    11761 aggtggcagg atcggcaccc agcgccgcac caagacgcga catttcagcc agcccacggg
    11821 tgatcagcgc cgtacgcgca ttcgcaccaa aaccgatacc gtcggacatc cccgcgccaa
    11881 tggcaatgac gtttttcacc gcaccgccaa gctgcacacc gatgaaatcc gggttgctgt
    11941 aaacgcggaa acttttgcca cagtgcagca attgttggag atcatcggca aaggtctggt
    12001 cggtagaggc caacgaaata gccgtcggta aacctgccgc cagttctttc gcaaacgttg
    12061 ggccagagat aaccgccagc ggaatttgat cgcctaaggc ctcacgcgcc acgtcctgta
    12121 acagacgtcc ggtttccgct tccagccctt tggtcgccca caccagacgc gcatcaggac
    12181 gcatcagcgg tttaatctgg cgcagcactt caccaaagac atggctgggt acgacgacga
    12241 gaatattacg gctggctgcc agcgcagtgg cgagatcgct ttcaagatgg agcgtatcgg
    12301 gaaaaggcac atcggggaga aacgcggcgt tacagcggtc gcgttcaagc gttgcgatat
    12361 gttcagggtc atggccccag aggacaacct cgtggccatt tcttgccagg gtaatggcaa
    12421 gagcggtgcc gtacgagccg gcaccgatca cagtcattga agcattacgt tggttcatca
    12481 ggcatcctga tgttcttcag taccttcgcc agcctgctgc tgcaaatagt tcatgaacag
    12541 cgcatcgaag ttaaccggcg caaggttcag ttgcgggaat gtaccgcggg ataccatgct
    12601 ggtgatgcac tcacgagcat acgggaacag aatgttcggg cagtatgcgc ccaggcaatg
    12661 tgccatctgg gtgccttcga tacccgcgat ggagaaaata ccgccctgct gaacttcaca
    12721 caggaacgcg gtttcttcgc ccagggaggc cgttacggta acacgcagta ccacttcgta
    12781 tacgtcatct gccagttggg aagatgccgt atccagatca agtttaactt ctggttgcca
    12841 atctttctgg aaaacgtgcg gcgcgttcgg cgcttcgaaa gagatatcct tggtataaat
    12901 acgttggatc tggaaagtca tttcagtgtt gttttgttct gacatgtgta gaaaaccctt
    12961 aagtgttgtc cttaaatact gcgcaatgta atgccaacgt tcacacgtta tttcagcagg
    13021 ggatccagtc caccacgtgc atccaatgca tacaagtcat cacagccgcc aatgtgctgt
    13081 gcgtcaataa aaatctgggg aaccgtggtg cgaccgctgc gtttgatcat ctcttcacgc
    13141 ttggcggcat tgccatcgat cggcaactcc tgaaaactca cgcccttgct gctcagcagt
    13201 gcttttgcac gatggcaata cgggcaggtt tctttggtat agatttcaac attggccata
    13261 atttagctcc tgttttttta ccctgaaatt tcatgttgca gggcggctgc aaaatggagt
    13321 gtccccagaa gcttataaca ataagtcgct gggggcctaa ttgcagctaa cggcctgcat
    13381 catgaaagac gacaggtaaa ttatttgccg cgcaccaaag gcaagttttc gccagcccag
    13441 ccagcgacgc cttctttcag cacgaatact tgcgcaaaac cagctttcgt cagcgcgttt
    13501 gcaggctcct ggcactgcat gccagaaccg tctatcacga taaccggttt gtctttgtgc
    13561 ttctcaagct caccaacatt gttggctttg atttcgctcg gcaacagatt gatagaacct
    13621 gcgatatggc ctttacggaa gtcatcacgc tgacgtaaat ccacaaccac agcgtcttct
    13681 ttgttgatca gacgcgtagc ttcaccacga gtaatcacct tcactttcga ggtcaggctc
    13741 ttaaacgtag tcacaagaac cgccaccagt aacgcgatcc aggcgatact cagtatggga
    13801 tgacggccaa caaattgcat aatttcttgc atggggggta acaactcccg acgtagtgat
    13861 taaaaaaacc aggaaaggag tatacctgcg cggtgcggca aatacagcca gcgcgttaac
    13921 tggaatgcaa ttttgcgggg cacgacgaaa aaaaaacgca aatctggcat cggcttgccg
    13981 cgagcggtcg tatattttga tcttcagagg ctattttatc gattcagctg tagtaaaatt
    14041 acgcaaattt tgactcttga gtatgaggtt gtcgcaatgt cggtttctaa aaaacctatg
    14101 gtactggtga ttctggatgg ctatggctat cgtgaagaac agcaggataa cgccattttt
    14161 agtgctaaaa ccccggtaat ggatgcactg tgggccaatc gtccgcatac cctgatcgac
    14221 gcttccggtc tggaagtcgg tctgcctgac cgtcagatgg gtaactccga agtaggccac
    14281 gttaacctgg gtgccggccg catcgtgtat caggacctga ctcgtctgga cgttgaaatc
    14341 aaagatcgcg ctttctttgc taatccagtg ctgactggcg cggtagataa agcgaaaaac
    14401 gcaggtaaag cagtacacat tatgggtctg ctctccgcag gcggcgtaca cagccacgaa
    14461 gatcacatca tggcgatggt agaactggca gctgaacgcg gcgcagaaaa aatctacctg
    14521 cacgcattcc ttgacggtcg cgacaccccg ccgcgcagtg ctgaatcctc gctgaaaaaa
    14581 tttgaagaga aatttgctgc gctgggtaaa ggccgcgtag cgtccatcat tggtcgttac
    14641 tacgcgatgg accgcgataa ccgttgggat cgcgttgaaa aagcctatga cctgctgact
    14701 ctggcgcagg gtgagttcca ggccgatact gccgttgctg gtttgcaggc agcttatgct
    14761 cgcgacgaaa acgacgagtt cgtgaaagcg accgttattc gtgcagaagg tcagccagat
    14821 gcggcaatgg aagacggcga tgcgctgatt ttcatgaact tccgtgctga ccgcgcgcgc
    14881 gaaatcactc gtgcattcgt aaacgcagat ttcgacggct tcgcgcgtaa gaaagtggtt
    14941 aacgtcgatt tcgtgatgct gaccgaatac gccgctgaca tcaaaactgc ggttgcttac
    15001 ccacccgctt ccctggttaa caccttcggc gagtggatgg cgaaaaacga caaaactcag
    15061 ttgcgtattt ccgaaaccga aaaatatgcc cacgttactt tcttcttcaa cggtggcgta
    15121 gaagagtcgt tcaaaggcga agatcgcatt ctgatcaact caccgaaagt ggctacctac
    15181 gatctgcaac cggaaatgag ctccgcagag ctgaccgaaa aactggttgc ggccatcaag
    15241 agcggcaaat acgacaccat tatctgtaac tatccgaacg gcgacatggt aggtcacacc
    15301 ggggtgatgg aagcggcggt taaagcggtt gaagcactgg atcactgcgt ggaagaagtc
    15361 gcgaaagcgg ttgagtccgt gggtggacaa ctgctgatca ccgctgacca cggtaacgct
    15421 gaacagatgc gcgatccggc aacgggtcag gcacacacgg cacacaccaa cctgccagtt
    15481 ccgctgattt acgttggtga taagaacgtg aaagcggttg caggcggcaa actttctgac
    15541 atcgcgccga ccatgttgtc gctgatgggt atggaaatcc cgcaagagat gactggtaag
    15601 ccgctgttca tcgtggaata atccctcccc atgaggggaa aggcgattaa taccatgaca
    15661 cgggccgtga aaccgcgcag gtttgcaatc aggcccatca tctacgccag cgttcttagc
    15721 gctggcgtat tgttgtgcgc cttttccgcc cacgcggatg agcgtgacca actcaaatct
    15781 attcaggccg atatcgccgc gaaagagcgc gcggtacgcc aaaagcaaca acaacgcgca
    15841 agcctgctcg cacaattgaa aaagcaggaa gaagcgatct ctgaagccac ccgtaagcta
    15901 cgcgaaacgc aaaacactct caatcaactc aataaacaga ttgatgagat gaacgcgtcg
    15961 attgccaaac tggagcagca aaaagccgcc caggagcgca gcctcgccgc acaactggat
    16021 gccgcattcc gtcagggcga gcataccggt attcagctaa ttctcagcgg tgaagaaagc
    16081 cagcgtggac agcgtttaca ggcttatttc ggctatctca accaggcgcg acaagaaacc
    16141 attgcccagt tgaagcaaac gcgtgaagaa gtcgccatgc agcgtgctga actggaagag
    16201 aaacaaagcg agcaacaaac gctgttatat gaacagcgcg cccaacaggc gaaactgact
    16261 caggctctga acgagcgtaa aaagacgctg gcggggctgg aatcttccat ccagcaaggt
    16321 cagcagcagc taagtgaact gcgtgccaac gaatcccgtc tgcgtaacag cattgcccgt
    16381 gcggaagccg cggcgaaagc gcgtgctgaa cgagaagcgc gtgaggccca ggcggttcgc
    16441 gaccgccaga aagaagcgac gcgcaaaggc accacctaca aaccgaccga aagcgaaaaa
    16501 tcgctgatgt cccgaactgg tggcctgggt gcgccgcgtg gtcaggcatt ctggccggtt
    16561 cgcgggccga cgctgcatcg ctatggtgaa cagctacagg gcgaactacg ctggaaagga
    16621 atggttatcg gtgcctctga aggtactgaa gttaaagcga ttgccgacgg tcgggtgatt
    16681 ctggctgact ggctgcaagg ttacggtctg gtggtggtgg ttgagcacgg taaaggcgac
    16741 atgagtcttt acggctataa tcagagcgca ctggtgagcg ttggttcgca ggttcgcgcg
    16801 ggccagccaa ttgcactggt gggcagcagt ggcggtcagg gtcggccttc actctatttc
    16861 gaaattcgcc gccagggtca ggcggtcaat ccacagccgt ggttgggaag ataagttttg
    16921 tttccatttc gtcgtaacgt tcttgcattt gccgctctgt tggcgctctc ctcccccgta
    16981 cttgctggca aacttgccat cgtcattgat gattttgggt atcgcccgca caacgaaaac
    17041 caggtgctgg cgatgccctc cgctatctcc gtcgctgtat tacccgattc accgcacgcc
    17101 agagagatgg cgaccaaagc gcataacagc gggcacgaag tgttgattca tctcccgatg
    17161 gcaccactga gtaaacagcc gctggagaaa aatacgctgc gcccggagat gagcagcgac
    17221 gaaattgagc gcattattcg tagtgcggtc aataacgtgc cctatgccgt ggggatcaac
    17281 aaccatatgg gtagcaagat gacctctaac ctgtttggta tgcagaaagt gatgcaggca
    17341 ctggagcgtt acaatcttta cttcctcgac agcgtaacca tcggtaatac ccaggcgatg
    17401 cgcgccgcgc aaggtactgg cgtgaaggtg atcaaacgga aggtgttcct cgacgattcg
    17461 cagaacgaag cggacatccg tgtgcaattt aatcgcgcaa ttgatctggc gcgtcgcaac
    17521 ggttcaacca ttgccattgg gcatcctcac ccttcaacgg tacgcgtgct acaacagatg
    17581 gtttataacc tgccgccaga cattacgctg gtgaaagcca gcagcttgct gaatgaaccg
    17641 caggttgata cttctacacc gccgaaaaac gctgtgcctg atgcaccgcg taatccgttc
    17701 cgtggcgtga agctgtgcaa accgaagaaa ccgatagaac ctgtttatgc taatcgcttc
    17761 tttgaagtat taagcgaaag catcagccag agcacgctga tcgtttactt ccagcatcag
    17821 tggcaaggct ggggcaaaca gcccgaagcg gcgaagttta acgctagcgc aaattaaagg
    17881 cgctacgcgt aatgcgatgg tgcgaccgcg ttttgtcgcg ccattgccat aagcggaaag
    17941 accacagtaa cgcctggtaa ccgaccttca cgctgcgcac attggtaatc aggcgcttat
    18001 acataccgga agtaaatatc tcggcaatca tccgttggcg ggtaagaata tccggctctt
    18061 tgcgcaccgc atggcaaaca cgcaatgctt cgtaagttat ttgctgatga aattccggat
    18121 aaatcgtaat tttgtcggca taatttcgat ttaatttctc cagcaggcgg gtaatcttaa
    18181 tatagtgacg ttgataatta aggtttttat tcccttgtct atgcaaccga ctcactgacg
    18241 tattatgc
//
LOCUS       DAZGSP010000100        18156 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZGSP010000100 DAZGSP010000000
VERSION     DAZGSP010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.101"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(119..925)
                     /gene="trpA"
                     /locus_tag="ACHK74_003865"
     CDS             complement(119..925)
                     /gene="trpA"
                     /locus_tag="ACHK74_003865"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFP0222603.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLVALKVFVQPMKAATRS"
     gene            complement(925..2118)
                     /gene="trpB"
                     /locus_tag="ACHK74_003866"
     CDS             complement(925..2118)
                     /gene="trpB"
                     /locus_tag="ACHK74_003866"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFP0222604.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2130..3491)
                     /gene="trpCF"
                     /locus_tag="ACHK74_003867"
     CDS             complement(2130..3491)
                     /gene="trpCF"
                     /locus_tag="ACHK74_003867"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFP0222605.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3492..5087)
                     /gene="trpD"
                     /locus_tag="ACHK74_003868"
     CDS             complement(3492..5087)
                     /gene="trpD"
                     /locus_tag="ACHK74_003868"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFP0222606.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5087..6649)
                     /gene="trpE"
                     /locus_tag="ACHK74_003869"
     CDS             complement(5087..6649)
                     /gene="trpE"
                     /locus_tag="ACHK74_003869"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFP0222607.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6671..6764)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6741..6785)
                     /gene="trpL"
                     /locus_tag="ACHK74_003870"
     CDS             complement(6741..6785)
                     /gene="trpL"
                     /locus_tag="ACHK74_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFP0222608.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6923..7804
                     /gene="rnm"
                     /locus_tag="ACHK74_003871"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6923..7804
                     /gene="rnm"
                     /locus_tag="ACHK74_003871"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFP0222609.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVK
                     GVWQLWEQPQNTTEREL"
     gene            7801..8421
                     /gene="yciO"
                     /locus_tag="ACHK74_003872"
     CDS             7801..8421
                     /gene="yciO"
                     /locus_tag="ACHK74_003872"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFP0222610.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8522..9394
                     /gene="rluB"
                     /locus_tag="ACHK74_003873"
     CDS             8522..9394
                     /gene="rluB"
                     /locus_tag="ACHK74_003873"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFP0222611.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(9434..10024)
                     /gene="cobO"
                     /locus_tag="ACHK74_003874"
     CDS             complement(9434..10024)
                     /gene="cobO"
                     /locus_tag="ACHK74_003874"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFP0222612.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(10021..10779)
                     /gene="yciK"
                     /locus_tag="ACHK74_003875"
     CDS             complement(10021..10779)
                     /gene="yciK"
                     /locus_tag="ACHK74_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFP0222613.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            10999..12048
                     /gene="sohB"
                     /locus_tag="ACHK74_003876"
     CDS             10999..12048
                     /gene="sohB"
                     /locus_tag="ACHK74_003876"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFP0222614.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(12084..12335)
                     /gene="yciN"
                     /locus_tag="ACHK74_003877"
     CDS             complement(12084..12335)
                     /gene="yciN"
                     /locus_tag="ACHK74_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFP0222615.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            12715..15312
                     /gene="topA"
                     /locus_tag="ACHK74_003878"
     CDS             12715..15312
                     /gene="topA"
                     /locus_tag="ACHK74_003878"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFP0222616.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            15522..16496
                     /gene="cysB"
                     /locus_tag="ACHK74_003879"
     CDS             15522..16496
                     /gene="cysB"
                     /locus_tag="ACHK74_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFP0222617.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            16815..16955
                     /gene="ymiA"
                     /locus_tag="ACHK74_003880"
     CDS             16815..16955
                     /gene="ymiA"
                     /locus_tag="ACHK74_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFP0222618.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            16958..17125
                     /gene="yciX"
                     /locus_tag="ACHK74_003881"
     CDS             16958..17125
                     /gene="yciX"
                     /locus_tag="ACHK74_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFP0222619.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            17239..17334
                     /gene="ymiC"
                     /locus_tag="ACHK74_003882"
     CDS             17239..17334
                     /gene="ymiC"
                     /locus_tag="ACHK74_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFP0222620.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            17498..>18156
                     /locus_tag="ACHK74_003883"
     CDS             17498..>18156
                     /locus_tag="ACHK74_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFP0222621.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDNEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NG"
BASE COUNT         4458 a         4562 c         4769 g         4367 t
ORIGIN      
        1 ttgacgttcg accggggtaa gcgaaacggt aaaaagataa atattaaatg aatttaggat
       61 ttttccggct tcattaaaga aagttaaaat gccgccagcg gaactggcgg ctgtgggatt
      121 aactgcgcgt cgccgctttc atcggttgta caaaaacttt cagtgccacc agcattttct
      181 ctggctcatt aatatgttgc tcgatgattt taacaatggc cgaaccagaa atcgcgcccg
      241 cagctcctgc atcaatcgct gcttttacct gatccggggc ggaaatacca aatccctgca
      301 atggaggtgc agcgttgtac tctttcagct tcgcaaccag atgattgagg ggtaacgcgg
      361 cgcggttttc tgcgccggtc acgcctgctc gtgacagcaa ataggtgtaa ccacgaccgt
      421 aagaggctat ctggcgcagc aggtcgtcat cggcatttgg cgggcagatg aagataggtg
      481 cgacattatg acgcaacgcg gcctggcgga agggcgcgga ctcttcaact ggcacatcgg
      541 caaccagcac cgaatcgacg ccgacttttt cgcactgggc ataaaactca tcaatgcctt
      601 tgttaaacac cagattggca tacatcaaca ggccaatggg aatggtcggg tgtttctggc
      661 gaatcagtgc cagcatttca aaacattgtg ccggagtcac acctgccgca aaagcgcgca
      721 gagtggcgtt ttgaatcgtc gggccatccg ccagtgggtc ggagaagggg atacctaact
      781 ccagcgcgtc agcaccggct tcaattagcg tatcgataat tttcaatgac tgctcaatgc
      841 ccggatcacc gagcgtgacg aaaggaacga atgcgccttc tttgcgctcc ttcaactggg
      901 caaacagaga ttcgtagcgt tccatcagat ttcccctcgt gctttcaaaa tatcgtgaac
      961 ggtgaagatg tctttatcgc cgcgaccgga aaggttaacc accagtagct gctctttatc
     1021 cgggttttcg cgcatcattt tcaacgcatg ggccagggcg tgggaggatt ccagcgccgg
     1081 gatgatccct tcgtgcaggc acagcgtttt gaaggcttca agggcttcat catcggtaat
     1141 agacacgtaa tcagcgcgtc cagtgctgtt aagatacgcg tgttgtgggc cgacagacgg
     1201 gaaatccagt ccggcggaga tggagtaaga ttcttcaatc tgcccgtctt cggtttgcat
     1261 catcggcgct ttcataccga aatagatacc cacgcgacca tgttttagcg gtgcgccgtg
     1321 ctcgccagtt tcgataccgt gaccacctgg ctccacacca atcaggccga cgttggtttc
     1381 attgatgaaa tcagcaaaca tgccgatggc attcgaaccg ccgccaacac aggcgataac
     1441 ggcatccggc aggcgacctt ctctttccag aatctgcgct ttggtttctt cgccaatcat
     1501 ccgctgaaac tcacgcacaa tggtcggata aggatgcggg ccagctgcgg tgcccagcat
     1561 atagtgcgcg gtttcgtaac taccggacca gtcgcgcagc gcctcgttac aggcatcttt
     1621 cagcgtcgcg gaaccgctat gcaccgggat cacttccgca cccattaagc gcatacgaaa
     1681 aacgttaggc gactggcgtt caacgtcttt ggcacccata taaatacggc atttcaggcc
     1741 gagcagggcg ctggcaaggg ccgacgccac gccatgctga ccggcaccgg tttcggcgat
     1801 gatttcggtt ttacccatcc gcttcgccag caacgcctgc cccagcacct ggttagtttt
     1861 atgcgcgccg ccgtgcagca aatcttcacg cttgagatac agcgtggtgt tcgtcccggc
     1921 tgtaatgttc tggcatttgg tcagcgcggt tggacgcccg gcatagtttt tcagcaggtc
     1981 gttgaactga gcctgaaatt caggatcttt ttgcgcactg acaaaagctt cttccagctg
     2041 gcgcagagca ggcatcagga tttgtggcac gtacatgccg ccaaactcac caaaataggg
     2101 gttaagtaat gttgtcattg ttcctttcct taatatgcgc gcagcgtctg gaaaaccgag
     2161 gccaaaagac gtgcgtcttt gatgcccggt tgcgactcta cagcagaatt aaaatcaagt
     2221 ccggcgcagc cggtttgtgc cgcttccacg cagttatctg cgcctaagcc ccccgccagc
     2281 agaacgttgc caagcgattg accatttaat agtgaccagt caaaacgttg cccgcttcca
     2341 ccctggccgt tgtctaaaac atatttatca acgtgctgaa actcgcgggc gggcagggtt
     2401 tcaccgacgc ttaatgcttt ccagatggca acatgtgctg gcagagcttc acgcagcgta
     2461 tcgatataca gctgttcttc attaccatgc agttgcactg ccgccagcga taacacctta
     2521 gctttgtcca ccacatcggc aatatcgtga ttgcggaaca cgccaacata ctgcaacggt
     2581 gccgcagcca tcacttcctg cgcctgttca acgttgacgc aacgcggtga tgtcgcaaca
     2641 aaaatcaacc caccgtaaat cgcgcccgcg tcataagctg ctttagcatc ttgcccacgc
     2701 gtcaggccac atactttatt ctcacccagc aacacccggc gcacggcggc gtgcaaatcg
     2761 tcatgggcca tcaacgccga accaatcaga aaaccgttag cgaagtggct taactcgcgc
     2821 acctgagcgt aagtattgat gccggattcg ctgattaccg ttacgttgtg ccccagtttc
     2881 ggcgcaagct cgcgggtacg gttgagatca atcgacaaat cacgcagatc gcggttgttg
     2941 atgccaacga cctttgcccc taatgcaatg gcgcgctcca gttcctcttc attactgact
     3001 tcggtcagca cccccatctc cagactgtga gcgacggcgg caagctggcg atattggtcg
     3061 tcatccagta ctgaaagcat taataagcag gcatcggcct ggtaatagcg cgccagatag
     3121 atctggtaag ggtcgataat gaagtcttta cataaaatcg gctgcggggc gatttggctg
     3181 acgatgggga ggaaatcaaa gctcccctga aaatatttct catcagtcag caccgaaatt
     3241 gccgaagcgt aatgtttata aatggcggca atgcgtgccg gatcgaaatc atcacggatc
     3301 acgccttttg acggcgacgc tttcttgcac tccagaataa acgccgtgcg tgcaccctgt
     3361 agcgcatcat aaaaatgtcg cgtgctcggc tgaacctcat tctgaaaact ggccagcggt
     3421 tgctgctgtt tgcgggtttc tacccaaatc gccttgtctg cgacgatttt cgctaaaacg
     3481 gtttgcatca tttaccctcg tgccgccagc gcggtaactc tgtcgtaagc ggaaccactg
     3541 cgcagtacct caagaacggt ttgcgcattg gcttgcagat cttcatggcc atgcaggcgc
     3601 attaacatgg cgacgttcgc agcgacggct gcttcatggg cggcgtcgcc tttaccttgt
     3661 aacaaacgtg ttaaaatgtc acggttttct tccggtgttc cgcctgccag ttgctcctgg
     3721 tggtagggtg tcaggccaaa atcttcagcg gtcaattgat agctcttaat ttcgccgtca
     3781 tgcagctcgg caacgattgt cggcgcgtgt aatgaaactt catccatccc gccgctgtgc
     3841 accaccgccg cgcgttgata ccccagcacg cgcaaggttt cggcaatcgg cagcaccagt
     3901 tccggactat aaacaccaat taacgccagc ggcggatgcg ccgggttaat caatggcccc
     3961 agcacattga acagggtgcg ggtttttagt tgctggcgaa ccggcattgc atggcgaaat
     4021 ccggtgtgat atttcggtgc aaagaggaaa catacgccta actcatccag cgcctggcgc
     4081 gatttatcgg cgttcatatc aagattaata ccgaacgccg ccagcagatc ggacgaacca
     4141 gatttactgg agacgctacg gttgccgtgt ttcgccactt tcagcccaca ggccgcggcg
     4201 acaaacgcac tggcggtaga aatattgata ctgttgctgc cgtcaccgcc agtaccgacg
     4261 atatcagcaa acagataatc cgggcgcggg aacggcgctg cgttttccag tagcgcggtt
     4321 gctgccccgg cgatctcgtt cgggtgctca ccgcgaattt tcatgctcac cagcgccgcc
     4381 gccagttgtt ccggcttcag ctcgccacgc accaccgctg aaaacagctg gtggctttct
     4441 tgttggctaa gcgtctgcgc ctgatacagt ttttccagaa tcggttgcag cgtgttggct
     4501 ggctctagtt tctgctgcgc ccaggccagc gtttgttcca gcaggcgagc gccctgggtg
     4561 gtgagaatgg attccggatg gaactggaat ccacaaacgc gatccgcatc gtgacgtact
     4621 gccatcacca tgccattaaa atgggcgttg atggttaaac cggccggaat gttactgcca
     4681 accagcgagt gataacgcgc caccggcagc gggtttgtta atccggcaaa catcgcctga
     4741 ccgtcatgtt caatgctcga cgctttaccg tgaagaattt cgcccgcctg accgacatag
     4801 cccccgtaag cttcgacaat tgcctgatgt ccgaggcaaa tgccaataat gggcagcttg
     4861 ccacgcaagc gggtgaggag ttccggcata caaccggctt cgctcggcac accggggcca
     4921 ggagaaagca tcagtaccgg attgctcatg gtcgccaggc gttcaattaa ggtctgcgcc
     4981 ggaatatggt tgcggtaaat caccacgtta tgcccattgc tgcgcaactg atctgccagg
     5041 ttgtacgtaa aagagtcgat attatcgagc agcagtatgt cagccatcag aaagtctcct
     5101 gtgcatgatg cgcggtggca atagcgcgca gtacagcgcg ggctttatta cgggtttcgt
     5161 cggcttccga ctgcggaata gaatcaagga ctacgccagc accggcttgc acggtggcga
     5221 taccgttttc caccagcgcc gagcggatca caatgcaggt gtcgagatcg ccgtgcgcgg
     5281 tgaaataacc taccgcgccg ccgtagctgc cgcggcgacg accttccgcc tcggcaatta
     5341 actgcatggc gcgcactttc ggcgcaccgc ttaacgtccc catattcata caggcgcggt
     5401 aagcgtgtag ggcgtcgaga tcgtggcgca gttcaccgac cacgcgggag accaggtgca
     5461 tcacgtaaga gtaacggtca actttggtga ggtcggcgac gtagcggctg ccgggggtgc
     5521 aaatgcgtgc cagatcatta cgggcaagat ccaccagcat cagatgttca gaaagctctt
     5581 tatgatcggt acgcatttcc agttcgatgc ggctgtcgag gtctctgtcc agcgagccat
     5641 cggcacgacg accgcgtgga cgtgttccgg caatcgggta gatctcaatc tggcggctgg
     5701 tggcgtcata cttgagcgaa ctttccggcg acgcgccaaa cagggtgaaa tcattatcct
     5761 gcataaaaaa catgtacggg ctgggattac tctttttcag cacgtaatag gccgccagcg
     5821 gtgacgggca gggcagagag aaacggcgag atggcaccac ctggaaaatt tctccagcgc
     5881 gaatcgcttt ttgcaacaaa cgcactacgc caccgaactc ttcatcgctc tgattacatt
     5941 cacaacgcat atgcggcacg gaaaccaccg gcagcggcgg cgcggcttcg gtcagttgct
     6001 gacgtagttc gttcaggcga gcagtgagac gttgtttttc ttcttcattc ggagcaaaca
     6061 ggctggcctg aatacgggtg ctttttttct gatggtcaat caccatcaga gtttcagcga
     6121 gataaaaaca gaaatcaggg cagttatttt ccgctgacag ttgcggtaaa tcttcaaatc
     6181 ccgccacaag gtcataagag aacaggccgc cgaagaacat tgcttctcgt tcttccttcg
     6241 gtacattcaa cagattctgc aataaacgga aagcgtcaaa aaccgaaagg gagcataagc
     6301 gagcgtcttc atccagcagt ggactgacag gggggaagcg cagcacacgg cagtttggta
     6361 attgttcatt ttccacaccc gcaggcaggg cgttatccag tagtgtcagc agggcttcgc
     6421 cgttgccgga aagtgcctgg attgtgacag tgtcacctaa agctgtaatg cgcagcgcac
     6481 tgtctaccag cagcaggctt tttaaatcat ctttgctgtc gatatctgcg gattccagca
     6541 gcagcgttgc cggacgatcc ccacacaact ggtgaaaaag cgcggtggga ttgtcgcgat
     6601 aagcgccttc gcaggttagc agttcgagag tcggtttttg tgtttgcatt gttattctct
     6661 aattttgttc aaaaaaaagc ccgctcatta ggcgggctgg gtatctgatt gctttacgca
     6721 tggtgaatac actgcccgtt tcaggaagtg cgccaccaac ctttcagtac gaaaattgct
     6781 ttcattgtcg ataccctttt tacgtgaact tgcgtactag ttaactagtt cgatgattaa
     6841 ttgtcaacag ctcatttcag aatatttgcc agaaccgtta tgatgtcggc gcaaaaaaca
     6901 ttatccagaa cgggagtgcg ccttgagcga cacgaattat gcagtgattt acgacctgca
     6961 cagccatacc acagcttccg atggctgcct gacgccagaa gcattggtgc accgtgcagt
     7021 cgagatgcgc gtcggcaccc tggcgatcac cgaccatgac accacagctg caatcgcgcc
     7081 agcaagagag gaaatttcac gttcgggact ggcgttgaat cttattcccg gcgtggaaat
     7141 ttccacggtc tgggaaaatc atgaaattca tattgtcggg ctgaatattg atattactca
     7201 tccgctaatg tgtgagttcc tcgcgcagca gacagaacgg cgcaatcagc gggcgcagct
     7261 gattgccgaa agacttgaaa aagcgcaaat acctggggcg ctggaaggcg cacaacgact
     7321 ggcgcagggc ggcgcagtga cgcgcggtca ttttgcgcgt ttccttgttg agtgcggcaa
     7381 agccagttca atggcggatg tctttaaaaa gtatctggcg cgcgggaaaa ccggatacgt
     7441 tccgccacag tggtgtacaa tagaacaagc tattgatgtc attcatcatt ctggcggtaa
     7501 ggcggtgcta gctcatcctg ggcggtacaa tcttagtgct aaatggctga aaagattggt
     7561 tgcgcatttt gccgaacacc acggtgacgc gatggaagtc gcgcagtgcc agcaatcgcc
     7621 caatgaacgc acccagctgg cgacacttgc gcgtcagcat catttatggg catcacaagg
     7681 atctgatttt catcagccat gcccgtggat cgaactgggt cgtaaactct ggctgcccgc
     7741 aggcgttaaa ggcgtctggc agctatggga acagccgcag aacaccacag agagggaatt
     7801 atgagccagt ttttttatat tcatcctgat aacccgcagc aacgtctgat caaccaggcg
     7861 gtggagatcg tgcgtaaagg cggggtgatt gtttatccaa ctgattccgg ctatgcgctc
     7921 ggctgtaaaa ttgaagataa aaacgccatg gagcgtattt gtcgtattcg ccagctgccg
     7981 gacggtcaca actttaccct gatgtgtcgc gatctttctg aactctcgac ctattcattt
     8041 gtcgataacg ttgcgtttcg tttaatgaaa aacaacacgc cgggcaacta taccttcatc
     8101 ctgaagggga cgaaggaggt gccacgccgt ctgttgcagg aaaaacgcaa aaccatcggt
     8161 atgcgtgtgc cttctaaccc gattgcgcag gcgttgcttg aagcactggg cgaaccgatg
     8221 ctttccactt cgctaatgct gccaggcagc gaatttaccg aatccgatcc cgaagaaatt
     8281 aaagaccgtc tggaaaaaca ggtagatttg attattcacg gcggttatct cgggcagaaa
     8341 ccaacaacgg ttattgatct taccgatgat acgccggttg tggtgcgtga aggcgtaggt
     8401 gatgtgaagc ctttcttata agtgcgtaac tctgtatact acgcggcctt aaaatggcgc
     8461 tatacggcgc cattgattct cctgttcgac gcctgtgaag gcgacactaa aaggaagctc
     8521 tatgagcgaa aaattacaga aagtgctggc ccgcgctggc cacggctccc gccgtgaaat
     8581 cgaatctatt attgaagccg gtcgtgtgag tgttgatggc aaaattgcta aactcggcga
     8641 tcgcgttgaa gttacccctg ggctgaaaat tcgtatcgat ggtcatctga tttcggtacg
     8701 tgagtccgct gaacaaattt gtcgcgttct ggcctattac aaaccggaag gtgagttgtg
     8761 tacccgtaac gatccggaag ggcgtccgac ggtgtttgac cgtctgccaa agctgcgtgg
     8821 cgcacgctgg attgccgtag gtcgtctgga cgttaacacc tgtggtctgc tgctgttcac
     8881 caccgatggt gaactggcaa accgtttaat gcatccaagc cgtgaagttg aacgtgaata
     8941 cgccgtgcgt gtatttggtc aggttgacga cgcgaaactg cgtgatttga gtcgtggcgt
     9001 gcaactggaa gatggtccgg cagcttttaa aaccatcaag ttcagcggcg gcgaagggat
     9061 caaccagtgg tacaacgtga ctctgaccga aggccgtaac cgtgaagttc gtcgtctgtg
     9121 ggaagcggtt ggtgtgcagg tgagccgcct gatccgtgtt cgttacggtg atatcccact
     9181 gccaaaaggt ctgccacgcg gtggctggac ggagctggac ctcgcccaga ctaactatct
     9241 gcgcgaactg gtggagctac cgccagaaac cagctctaaa gtcgctgtag aaaaagaccg
     9301 tcgtcgcatg aaggcgaatc agattcgtcg tgcggtgaaa cgtcacagtc aggtgagcgg
     9361 cggtcgtcgt tctggcgggc gtaataacgg ttaatcaaag tatgccggac gtcatatccg
     9421 gcatttttac agattaataa tcaatcccta tttgcgcctt aacaccggca tcaaacgcat
     9481 gtttgatggg gcgcagctca cttaccgtat ccgccagttc aagaatatcc cgatgacagc
     9541 ctcgacccgt gataatcacc gtctgttgat gtggacgttc atttaacgcc tgcaccactt
     9601 cttccagtgg caaatagtca tacgccacca tatacgtcag ttcatcaagc aaaaccatat
     9661 ccagtgagga atcagcaagc atccgctttg catgttgcca gacttcgcgg caggcggcgg
     9721 tatcagactc gcggttttgt gtatcccagg taaagcccgt tgccatcacc tgaaactcaa
     9781 cgccatgtgg ctccagcaga ttgcgttcgc cattaggcca ggtgccttta ataaactgca
     9841 cgacgcccac tttttttccg tgaccaactg cgcgtgttgc cgtaccaaat gccgcggtgg
     9901 ttttgccttt tccattgccg gtaaagacga tgataatacc gcgttcatcc tgggcctggg
     9961 ccacacgagc atctactttt tctttcactc gctgctgacg ctgttggtag cgttcatcac
    10021 tcattgggaa attcctggtt tacggcccgg ttgggcgtca aaggtcatgc cggttttacg
    10081 gcggctgtca tcgcccatca gccagaggta gagcggcatg atatcagcgg gtgttttaag
    10141 tttctgtgga tcttcggtcg ggaaggcgct tgcgcgcatt gcggtgcgcg taccgcctgg
    10201 gttaatgcag ttgacgcgca ggcgctgctg atattcatcg gccagtacct gcatcatccc
    10261 ttcggtggca aatttcgacg ctgcatatgc accccagtta gctcgtccct gacgtccaac
    10321 gcttgatgaa gtaaagacca gtgaaccggc gtccgatttg agtaataaag gaagcagtgc
    10381 ctgggtgagc ataaaggtgg cattaacgtt gacctgcatg acgtcctgcc agacctgcgg
    10441 attttgttcg ctcattgggc aaatatcgcc gagcaatccg gcattatgca aaacaccatc
    10501 cagacgcgga taattaacga caatgcgctg tgccagttgt tggcaatctt cggacgtgca
    10561 ggtcagcaaa tcgagaataa accactgtgg ctgacgtcca gtttcttcgt ttatgtggct
    10621 ggctacctga cgtaattttt cttcattacg gcccaacaga atcactgtcg caccatagcg
    10681 tgcatacgtc atcgcggctt cacggccaat cccatcgctg gctcccgtca ccaggataat
    10741 gcgatcattg agtaaatctt gttttggctg gtaatgcatg gctactcctc aacgacgttg
    10801 tctgtcgcgc tgccagtgta ctttatgact ttggggcttt atgcctgaaa cagaagggca
    10861 tttcaatcag cctggggcga taacaccgca aaatggatac tttgtcatac tttcgctgca
    10921 ataacatctc tgcgagacgg cttaacatgc ctgttgtaaa ctgtgagcca aagcgttgtt
    10981 taaccaaggt ggggactcgt ggaattgttg tctgaatatg gtttgttttt ggcgaaaatc
    11041 gttaccgttg tgctagcgat tgcggcgatt gccgccatta ttgtcaatgt tgctcaacgt
    11101 aataaacgcc agcgtggcga gttacgggtc aacaatctca gcgaacagta taaggagatg
    11161 aaagaagaac tggccgcggc gctgatggac tcacatcagc aaaaacagtg gcacaaagcg
    11221 cagaagaaaa agcacaagca agaagcgaaa gcagcaaaag cgaaagccaa actgggcgag
    11281 gtggcaactg acagtaaacc tcgcgtctgg gtgctggatt ttaaaggcag catggacgcc
    11341 catgaagtga actcgctacg tgaagagata acggctgtac tcgcagcatt caaaccgcag
    11401 gatcaggttg tgctacgtct ggaaagccct ggtggcatgg tgcatggtta cgggttggcg
    11461 gcttcgcagc tgcagcgtct gcgtgataaa aacattcctt taactgttac ggtagacaaa
    11521 gtcgctgcca gcggcggtta catgatggcc tgtgtggcgg acaaaattgt ttccgcaccg
    11581 tttgctattg tgggttccat tggggtggtg gcgcaaatgc ccaactttaa ccgcttcctg
    11641 aaaagcaaag atattgatat cgaactgcac actgccgggc agtataagcg tactctgacg
    11701 ttgctgggtg aaaataccga agaagggcgg gagaaattcc gcgaagagtt gaacgaaacg
    11761 catcagttgt ttaaagattt tgtgaagcgt atgcgtccgt ctctggatat tgaacaggtg
    11821 gcaacgggtg aacactggta cggacaacag gcggtagaga aaggcctggt tgatgaaatc
    11881 aataccagtg atgaagttat tcttagcctg atggaaggcc gtgaagtggt taatgtacgc
    11941 tatatgcagc gtaaacgact cattgaccga ttcaccggca gcgcggcaga gagcgccgat
    12001 cgattgttgc tacgctggtg gcagcggggt caaaagccat tgatgtaaaa gacaaacgcg
    12061 aggctaagac ctcgcgtttt gctttaatca accagatgat atttttctga aagcacatgg
    12121 gccaggtgtt tgaacatatt aaacaccgcg gtgcttttgg ctgttggcaa tccttgttca
    12181 tctaaaaagt agtcgccggt aaataccagc acgccattac gctgcgtgac gccggtggct
    12241 tcaatccctg cgagcgtatc ctcatgctca cgaatgattt tgttggcctc tttcaacagc
    12301 gtttcgcggt cgatgggttg tgtctctttg ttcattattt actccttaaa caaggacatt
    12361 agtctacgcc aggcatggct tgcagacaaa tataccacgc tggtggcaag agcgccttac
    12421 tggcaacttt ggattttgca tgctaataaa gttgcgtatc ggattttatc aggtacagtg
    12481 tgacgctttc gtcaatctgg caatagattt gcttgacatt cgaccaaaat tccgtcgtgc
    12541 tatagcgcct gtaggccaag acctgttaac tcagtcacct gaattttcgt gaacagagtc
    12601 acgacaaggg gttgatatcc gcagagaacg agtccatatc ggtaactcgt tgccagtgga
    12661 aggtttatca acgtgcgacg cattcctgga agaatcaaat taggtaaggt gaatatgggt
    12721 aaagctcttg tcatcgttga gtccccggca aaagccaaaa cgatcaacaa gtatctgggt
    12781 agtgactacg tggtgaaatc cagcgtcggt cacatccgcg atttgccgac cagtggctca
    12841 gctgccaaaa agagtgccga ctctacctcc accaagacgg ctaaaaagcc taaaaaggat
    12901 gaacgtggcg ctctcgtcaa ccgtatgggg gttgacccgt ggcacaattg ggaggcgcac
    12961 tatgaagtgt tgcctggtaa agagaaggtc gtctctgaac tgaaacaact ggctgaaaaa
    13021 gccgaccaca tctatctcgc aaccgacctt gaccgcgaag gggaagccat tgcatggcac
    13081 ctgcgggaag tgattggggg tgatgatgcg cgctatagcc gagtggtgtt taacgaaatt
    13141 actaaaaacg cgatccgcca ggcatttaat aaaccgggtg agctgaatat tgatcgtgtt
    13201 aatgcccagc aggcgcgtcg ctttatggac cgcgtggtgg ggtatatggt ttcgccgctg
    13261 ctatggaaaa agatcgctcg tggtctgtct gccggtcgtg tgcagtcggt ggcggttcgc
    13321 ctggtggtcg agcgtgagcg tgaaattaaa gcgttcgtgc cggaagagtt ctgggaagtc
    13381 gatgccagca cgaccacgcc atctggtgaa gcgttggcgt tacaggtgac tcatcagaac
    13441 gacaaaccgt tccgtccggt caacaaagaa caaactcagg ctgcggtaag tctgctggaa
    13501 aaagcgcgct acagcgtgct ggaacgtgaa gacaaaccga caaccagtaa acctggcgct
    13561 ccttttatta cctctacgct gcaacaagct gccagcaccc gtcttggatt tggcgtgaaa
    13621 aaaaccatga tgatggcgca gcgtttgtat gaagcaggct atatcactta catgcgtacc
    13681 gactccacta acctgagtca ggacgcggta aatatggttc gcggttatat cagcgataat
    13741 tttggtaaga aatatctgcc ggaaagtccg aatcagtacg ccagcaaaga aaactcacag
    13801 gaagcgcacg aagcgattcg tccttctgac gtcaatgtga tggcggaatc gctgaaggat
    13861 atggaagcag atgcgcagaa actgtaccag ttaatctggc gtcagttcgt agcctgccag
    13921 atgaccccag cgaaatatga ctccacgacg ctgaccgttg gtgcgggcga tttccgcctg
    13981 aaagcacgcg gtcgtatttt gcgttttgat ggctggacaa aagtgatgcc tgcgttgcgt
    14041 aaaggcgatg aagatcgcat cttaccagca gttaataaag gcgatgctct gacgctcgtt
    14101 gaacttacac cagcccagca ctttaccaag ccgccagccc gtttcagtga agcatcgctg
    14161 gttaaagagc tggaaaaacg cggtatcggt cgtccgtcta cctatgcgtc gatcatttcg
    14221 accattcaag atcgtggcta cgtgcgagta gaaaatcgtc gtttctatgc ggaaaaaatg
    14281 ggcgaaatcg tcaccgatcg ccttgaagaa aatttccgcg agttaatgaa ctacgacttt
    14341 accgcgcaga tggaaaacag cctcgaccag gtggcaaatc acgaagcaga gtggaaagct
    14401 gtactggatc acttcttctc ggatttcacc cagcagttag ataaagctga aaaagatccg
    14461 gaagagggtg gtatgcgccc gaaccagatg gttctgacca gcattgactg cccgacttgt
    14521 ggtcgcaaaa tggggattcg cacagcgagc accggggtat tccttggctg ttctggttat
    14581 gcgctgccgc cgaaagagcg ttgcaaaacc accattaacc tggtgccgga aaacgaagtg
    14641 ctgaacgtac tggaaggcga agacgctgaa accaacgcgc tgcgcgcaaa acgtcgttgc
    14701 ccgaaatgcg gcacggcgat ggacagctat ctcatcgatc cgaaacgtaa gttgcatgtc
    14761 tgtggtaata acccaacctg cgacggttac gagatcgaag agggcgaatt ccgcattaaa
    14821 ggttatgacg gcccgatcgt tgagtgtgaa aaatgtggct ctgaaatgca cctgaaaatg
    14881 gggcgtttcg gtaaatacat ggcctgcacc aacgaagagt gtaaaaacac acgtaagatt
    14941 ttacgtaacg gcgaagtggc accaccgaaa gaagatccgg tgccattacc tgagctgccg
    15001 tgcgaaaaat cagatgctta tttcgtgctg cgtgacggtg ctgccggtgt gttcctggct
    15061 gccaacactt tcccgaaatc gcgtgaaacg cgtgcgccac tggtggaaga gctttatcgc
    15121 ttccgcgacc gtctgccgga aaaactgcgt tatctggccg atgcgccaca gcaggatccg
    15181 gaaggtaata agaccatggt tcgctttagc cgtaaaacca aacagcaata tgtctcttcg
    15241 gaaaaagacg gaaaggcgac tggctggtca gcattttatg ttgatggcaa atgggttgaa
    15301 ggaaaaaaat aacctttaat tctgtcaggt ttttataaac aaagggtcgc gaaagcggcc
    15361 cttttttatt gcatattatt ttttcttcac acctatacac taaggctata aatgatatag
    15421 tggttatagt tagcaccttt tttattatta aatcgtatta gtcacccgcc aggtgtgacg
    15481 aaaaaacgat gttctgatgg cgtctaagtg gatggtttaa catgaaatta caacaacttc
    15541 gctatattgt tgaggtggtc aatcataacc tgaatgtctc atcaacagcg gaaggacttt
    15601 acacatcaca acccgggatc agtaaacaag tcagaatgct ggaagacgag ctaggcattc
    15661 aaattttttc ccgaagcggc aagcacctga cgcaggtaac gccagcagga caagaaataa
    15721 ttcgtatcgc tcgcgaagtc ctgtcgaaag tcgatgccat aaaatcggtt gccggagagc
    15781 acacctggcc ggataaaggc tcgctgtata tcgccaccac gcatacccag gcacgctacg
    15841 cattaccaaa cgtcatcaaa ggctttattg agcgttatcc tcgcgtttct ttgcatatgc
    15901 accagggctc gccgacacaa attgctgatg ccgtctctaa aggcaatgcc gatttcgcta
    15961 tcgccacaga agcgctgcat ctgtatgaag atttagtgat gttaccgtgc taccactgga
    16021 atcgggctat tgtagtcact ccggatcacc cgctggcagg caaaaaagcc attaccattg
    16081 aagaactggc gcaatatccg ttggtgacat ataccttcgg ctttaccgga cgttcagaac
    16141 tggatactgc ctttaaccgc gcagggttaa cgccgcgtat cgtcttcacg gcaacggatg
    16201 ctgacgtcat taaaacttac gtccggttag ggctgggggt aggggtcatt gccagcatgg
    16261 cggtggatcc ggtcgccgat cccgaccttg tgcgtgttga tgctcacgat atcttcagcc
    16321 acagtacaac caaaattggt tttcgccgta gtactttctt gcgcagttat atgtatgatt
    16381 tcattcagcg ttttgcaccg catttaacgc gtgatgtcgt tgatgcggct gtcgcattgc
    16441 gctctaatga agaaattgag gtcatgttta aagatataaa actgccggaa aaataatttc
    16501 ttgcgttatt ttcggcacct tttatgtagc gaaggtgccg gaatatattc tcttttgtta
    16561 cttatttcta taccgtgcac aatattttta tccctcatat ttatagggta aattacctgt
    16621 aaagtagtgt ctaattaccc gcctcgcaaa ctccagtttt caaatatcta tttcaggtca
    16681 aaagattgaa ttattcactg gagacgattc gtaaattcgc tggatttttg actaaagttt
    16741 ctttaggatt tgtctcatcg tatgattaat tacaccaatc gttcaacgtc ttgctgataa
    16801 gtgatggcga ttatatgagg ttagcaatgc cttctggaaa tcaggaaccc cgccgcgatc
    16861 ctgaattaaa acgtaaagcc tggctggcgg tttttcttgg ttctgcactt ttctgggtgg
    16921 ttgtcgcact gctgatttgg aaagtgtggg gataactatg gtcggtcagg agcaactgga
    16981 gtcgtcacca ttatgccagc atagtgacaa tgaaacagaa acgaaacggg aatgttccgt
    17041 cgttattcca gacgactggc aactaacatc gcagcagcaa gcctttatag aactgtttgc
    17101 tgaagatgat cagccgaaac aataattatc atcattctta ttacccattt ttaatgaatt
    17161 aaagggcttt taatacaccg cagcaataac agcttgagtt atctcaacac aaaataataa
    17221 ccgttaaggg tgtagcctat gatcaacaca aatatgaaat attggtcctg gatgggcgcg
    17281 ttttctctgt cgatgctctt ctgggccgaa ctcctctgga tcattactca ctgatccttg
    17341 accccgctgc ggcggggttg tcatttgctt tgccacaagg tttctcctct tttatcaatt
    17401 tgggttgtta tcaaatcgtt acgcgatgtt tgtgttatct ttaatattca ccctgaagag
    17461 aatcagggct tcgcaaccct gtcattaagg aggagctatg tcgtcaaccc tacgagaagc
    17521 cagtaaggac acgttgcagg ccaaagataa aacttaccac tactacagcc tgccgcttgc
    17581 tgctaaatca ctgggcgata tcacccgtct acccaagtca ctcaaagttt tgctcgaaaa
    17641 cctgctgcgc tggcaggatg gtaactcggt taccgaagag gatatccacg cgctggcagg
    17701 atggctgaaa aatgcccatg ctgaccgtga aattgcctac cgcccggcaa gggtgctgat
    17761 gcaggacttt accggcgtac ctgccgttgt tgatctggcg gcaatgcgcg aagcggttaa
    17821 acgcctcggc ggcgatactg caaaggttaa cccgctctca ccggtcgacc tggtcattga
    17881 ccactcggtg accgtcgatc gttttggtga taatgaggca tttgaagaaa acgtacgcct
    17941 ggaaatggag cgcaaccacg aacgttatgt gttcctgaaa tggggaaagc aagcgttcag
    18001 tcggtttagc gtcgtgccgc caggcacagg catttgccat caggttaacc tcgaatatct
    18061 cggcaaagca gtgtggagtg aattgcagga cggtgaatgg attgcttatc cggatacact
    18121 cgttggtact gactcgcaca ccactatgat caacgg
//
LOCUS       DAZGSP010000101        17944 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZGSP010000101 DAZGSP010000000
VERSION     DAZGSP010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.102"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..4618
                     /locus_tag="ACHK74_003884"
     CDS             <1..4618
                     /locus_tag="ACHK74_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
                     /protein_id="HFP0222622.1"
                     /translation="IGGNATGVGTLNVQDQDSVITVRRLYNGYFGNGAVNISNNGLIN
                     NKEYSLVGVEDGSHGVVNVTDKGHWSFLGTGEAFRYIYIGDAGDGELNVSREGKVDSG
                     IITAGMKETGTGNITVKDKNSVITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGE
                     NGVGNVSITTGGMWEVNKNVYTTIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTL
                     NLMDGTSSFDTVGINVGNFGSGIVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLW
                     NLKTSSTNAQLLQVGVLGTGELNITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLET
                     FNMYVGTSGTGTLTLTNSGTLNVEGGEVYLGVFEPAVGTLNIGAAHGEAAADAGYITN
                     ATKVEFGSGEGVFVFNHTNNSDAGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGK
                     TLVNDGLLTIASHTADGVTGMGSSEVTIASPGTLDILASTNSAGDYTLTNALKGDGLM
                     RVQLSSSDKMFGFTHATGTEFAGVAQLKDSTFTLERDNTAALTHAMLQSDSENTTSVK
                     VGEQSIGGLAMNGGTLIFDTDIPAATLAEGYISVDTLVVGAGDYTWKGRNYQVNGTGD
                     VLIDVPKPWNDPMANNPLTTLNLLEHDDSHVGVQLVKAQTVIGSGGSLTLRDLQGDEV
                     EADKTLHIAQNGTVVAEGDYGFRLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAY
                     GATADMSAKIGGEGDLAINTVRQVSLSNGQNDYQGATYVQMGTLRTDADGALGNTREL
                     NISNAAIVDLNGSTQTVETFTGQMGSTVLFKEGALTVNKGGISQGELTGGGNLNVTGG
                     TLAIEGLNARYNALTSISPNAEVSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGK
                     GIVSATARTDVELDGDNSRFVGQFNIDTGSALSVNEQKNLGDASVINNGLLTISTERS
                     WAMTHSISGSGDVTKLGTGILTLNNDSAAYQGTTDIVGGEIAFGSDSAINMASQHINI
                     HNSGVMSGNVTTAGDVNVMPRGTLRVAKTTIGGNLENGGTVQMNSEGGKPGNVLTVNG
                     NYTGNNGLMTFNATLGGDNSPTDKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEV
                     GGNSAGNFALTTGTVEAGAYVYTLAKGKGNDEKNWYLTSKWDGVTPPDTPDPINNPPV
                     VDPEGPSVYRPEAGSYISNIAAANSLFSHRLHDRLGEPQYTDSLHSQGSASSMWMRHV
                     GGHERSRAGDGQLNTQANRYVLQLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNR
                     VGYKSDGRISGYSAGIYATWYQNDANKTGAYVDSWALYNWFDNSVSSDNRSADDYDSR
                     GVTASVEGGYTFEAGTFSGSEGTLNTWYVQPQVQITWMGVKDSDHTRKDGTRIETEGD
                     GNVQTRLGVKTYLNSHHQRDDGKQREFQPYIEANWINNSKVYAVKMNGQTVSRDGARN
                     LGEVRTGVEAKVNNNLSLWGNVGVQLGDKGYSDTQGMLGVKYSW"
     gene            4827..5093
                     /gene="hipB"
                     /locus_tag="ACHK74_003885"
     CDS             4827..5093
                     /gene="hipB"
                     /locus_tag="ACHK74_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFP0222623.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            5093..6415
                     /gene="hipA"
                     /locus_tag="ACHK74_003886"
     CDS             5093..6415
                     /gene="hipA"
                     /locus_tag="ACHK74_003886"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFP0222624.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            6727..6935
                     /locus_tag="ACHK74_003887"
                     /pseudo
     CDS             6727..6935
                     /locus_tag="ACHK74_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7367..7930
                     /gene="fimA"
                     /locus_tag="ACHK74_003888"
     CDS             7367..7930
                     /gene="fimA"
                     /locus_tag="ACHK74_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP0222625.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            8291..9001
                     /locus_tag="ACHK74_003889"
     CDS             8291..9001
                     /locus_tag="ACHK74_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0222626.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            9043..11693
                     /locus_tag="ACHK74_003890"
                     /pseudo
     CDS             9043..11693
                     /locus_tag="ACHK74_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            11707..12237
                     /gene="ydeS"
                     /locus_tag="ACHK74_003891"
     CDS             11707..12237
                     /gene="ydeS"
                     /locus_tag="ACHK74_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0222627.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            12250..12753
                     /gene="ydeR"
                     /locus_tag="ACHK74_003892"
     CDS             12250..12753
                     /gene="ydeR"
                     /locus_tag="ACHK74_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0222628.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            12813..13727
                     /gene="ydeQ"
                     /locus_tag="ACHK74_003893"
     CDS             12813..13727
                     /gene="ydeQ"
                     /locus_tag="ACHK74_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0222629.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            14061..16340
                     /gene="ydeP"
                     /locus_tag="ACHK74_003894"
     CDS             14061..16340
                     /gene="ydeP"
                     /locus_tag="ACHK74_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFP0222630.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPQAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            16588..16785
                     /gene="safA"
                     /locus_tag="ACHK74_003895"
     CDS             16588..16785
                     /gene="safA"
                     /locus_tag="ACHK74_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFP0222631.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            16860..17621
                     /gene="ydeO"
                     /locus_tag="ACHK74_003896"
     CDS             16860..17621
                     /gene="ydeO"
                     /locus_tag="ACHK74_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFP0222632.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMNALAI"
BASE COUNT         5048 a         3849 c         4354 g         4693 t
ORIGIN      
        1 tatcggtggc aatgccaccg gtgtcggaac cctgaatgtg caggatcaag actctgtcat
       61 cacggtacgc agactctata atggatattt cggtaatggc gcagtcaata tttccaataa
      121 tggactaatt aataacaaag aatattcatt ggtgggcgtt gaagacggtt cccacggtgt
      181 cgtcaacgtg accgataaag ggcattggag tttcctcgga acaggcgaag ctttccgcta
      241 tatctatatc ggtgatgctg gtgacggtga acttaatgtc tcgcgtgaag gtaaagtaga
      301 ttcgggaatt atcactgcgg ggatgaaaga aacaggcaca ggcaacatta ctgttaagga
      361 taagaactcc gttatcacta atctcggaac taatcttggt tatgacggcc acggcgaaat
      421 gaatatcagt aatgaggggc ttgttgtcag caacggagga agctcactcg gttatggaga
      481 aaacggcgtc gggaatgtca gcatcaccac gggggggatg tgggaggtca ataagaatgt
      541 ctataccacc attggtgttg cgggcgtcgg aaacctcaat attagcgatg gcggtaagtt
      601 tgtatcgcaa aatattactt ttttgggcga taaagcaagc ggtatcggca cactgaacct
      661 gatggatggg acatcatcgt tcgatactgt gggtatcaat gtcggtaatt ttggtagcgg
      721 tatcgtaaat gtcagtaatg gtgccaccct taattcaacg ggctatggat ttatcggagg
      781 aaatgcctcc ggtaaaggga tagttaatat ttcaacggat agtctctgga atttaaagac
      841 atcatctact aacgcccaat tgctacaggt cggtgtatta ggcacgggtg aactgaatat
      901 taccaccgga ggtatagtta aagcgcgtga tacacagata gctctcaatg acaaaagtaa
      961 gggcgacgtg agggtggatg ggcagaactc tcttcttgaa acattcaata tgtacgtagg
     1021 gacatctggt acgggtacgt taaccctgac gaatagcggc acgctgaatg tcgaaggtgg
     1081 agaagtttac ttaggtgttt ttgaacctgc tgtaggaacg ctaaacattg gtgcagctca
     1141 cggtgaggcg gcggcagatg ccgggtatat aaccaacgcg acgaaagtag agtttggttc
     1201 tggcgaaggt gtttttgtct ttaatcatac taataacagt gatgccggct accaggtcga
     1261 tatgctgatt acgggtgacg ataaagacgg aaaagtgatc catgatgcag gccatacggt
     1321 gttcaatgca gggaatactt atagcggtaa aacgctggtc aatgacggcc tcctgaccat
     1381 tgcgtctcat acggcagatg gggtaacggg catgggttcg agtgaagtca ccattgcaag
     1441 ccccggtacg ctcgacattc tcgcatcaac gaacagtgca ggagattaca cgctgaccaa
     1501 tgcgctcaaa ggcgatggtt tgatgcgagt gcagctgtca tcctccgaca agatgtttgg
     1561 ctttacccat gcaacaggga ctgaattcgc cggtgttgcc caactgaaag acagtacctt
     1621 cactctggaa cgcgacaaca ccgctgcgct tactcacgcg atgttgcagt ctgacagtga
     1681 aaataccaca tcggtaaaag tgggagagca atccattggt ggactggcca tgaatggcgg
     1741 tacgctcatt ttcgatacgg atattcctgc tgcgacgctt gcagagggat atatcagcgt
     1801 cgatacgctg gttgtcggcg cgggtgacta cacctggaaa ggccgtaact atcaggtaaa
     1861 cgggacgggc gacgtgctta tcgacgtgcc taaaccgtgg aatgatccca tggcgaataa
     1921 ccctctgacg acgctcaatt tgctggaaca cgacgatagc catgtcggcg ttcaactggt
     1981 gaaggcgcaa acggttattg ggtcgggtgg ctcattaacg ttacgtgatt tacagggcga
     2041 cgaggtggaa gcggacaaaa cgttacacat tgcgcaaaac ggaacggtgg tcgccgaggg
     2101 tgattatgga ttccgcctca cgaccgcacc aggtgatggt ttgtacgtta actatgggct
     2161 gaaagcgctg aacatccatg gtgggcaaaa gctgacgtta gccgaacatg gcggagccta
     2221 tggcgcaacg gccgatatgt cggcaaaaat cggtggtgaa ggggatctgg caatcaatac
     2281 ggtgcgacag gtttcgcttt ccaacggtca gaacgactat cagggggcaa cctacgttca
     2341 gatggggaca ttacgtaccg atgcggatgg tgcacttggc aacacccggg aactgaacat
     2401 cagcaacgcg gccatcgtcg atcttaatgg atcgacgcag acggtagaga cattcaccgg
     2461 gcagatgggt tcgactgttt tgttcaaaga gggggcgctg acggtaaata aaggtgggat
     2521 cagtcagggt gaactgacag gtggcggaaa cctgaatgtt acagggggaa cgctggctat
     2581 cgaggggctt aatgcacgct acaatgcgtt aaccagcatt agcccaaatg cggaagtcag
     2641 cctcgataat actcaggggt taggcagagg aaatattgcc aatgacggtc tgttaacgct
     2701 aaaaaacgtg actggcgaac tgcgtaatag cataagcggg aagggtatcg tgagcgcaac
     2761 cgccaggaca gatgtagagc tggatggcga taatagccgc tttgtggggc aattcaacat
     2821 tgatacaggc agcgcgctca gcgtcaacga gcagaaaaac ctgggtgatg cttccgttat
     2881 caataatggc ctgctcacca tctccactga gcgtagctgg gcgatgacgc acagtatcag
     2941 cggtagcggt gatgtgacaa aactgggtac cgggatcctg actcttaaca acgattccgc
     3001 ggcgtatcag ggtacgacgg atatcgtggg gggggaaatt gctttcggtt ccgactctgc
     3061 cattaatatg gcaagtcaac acattaatat ccataacagc ggtgtgatgt cgggaaatgt
     3121 caccactgca ggtgatgtga acgttatgcc tagggggaca ctgcgtgtcg ctaaaaccac
     3181 aatcggcggc aacctggaga atggcggcac ggttcaaatg aatagcgaag gggggaaacc
     3241 ggggaatgta ctgaccgtta acggcaacta taccggaaac aatggcctga tgacgttcaa
     3301 cgcgacgctg ggcggcgata attcgcccac cgataagatg aacgtgaaag gcgataccca
     3361 agggaacact cgcgttcggg ttgataacat tggcggcgtc ggtgcacaaa cggtcaacgg
     3421 tattgaactc attgaggttg gcggtaattc tgcaggtaac ttcgcgctga caaccggaac
     3481 tgtcgaagct ggggcttacg tctacacgct ggctaaaggg aaggggaatg acgagaaaaa
     3541 ctggtatctg accagtaaat gggacggcgt aacgccaccg gatacacccg accccatcaa
     3601 taatccccct gttgtggatc cggaaggccc atcagtttat cgcccggagg ccggaagcta
     3661 tatcagcaac attgccgcag ccaactcgct gtttagccat cgtttacacg accgtttggg
     3721 tgagccgcag tatacagatt cactgcattc tcaagggtcg gcaagcagta tgtggatgcg
     3781 tcatgtcgga gggcacgaac gttcaagggc cggtgacggt cagctaaata ctcaggctaa
     3841 ccgctatgta ttgcagctag gcggcgattt ggcgcagtgg agtagcaacg cgcaggatcg
     3901 ctggcatctt ggcgtgatgg caggctacgc caatcagcac agtaatactc agagtaatcg
     3961 tgtgggttat aaatcggatg ggcgcatcag cgggtacagc gctgggattt acgcgacctg
     4021 gtatcagaac gatgcgaata agaccggcgc ttatgttgac agctgggcgc tgtataactg
     4081 gtttgataac agcgtcagtt ccgataaccg ttctgctgac gactatgatt ctcgcggtgt
     4141 gacggcctct gttgagggtg ggtatacctt tgaagcggga acatttagcg gcagcgaagg
     4201 gacgctgaat acctggtacg tccagccaca ggtgcaaatc acctggatgg gtgtgaaaga
     4261 ttctgaccat acccggaaag acggaacacg cattgaaacg gaaggcgacg gaaacgtgca
     4321 aacgcgactt ggggtgaaaa cctacctgaa cagccatcac cagcgtgacg atggtaaaca
     4381 gcgtgagttc cagccttaca ttgaagcgaa ctggatcaac aatagcaaag tctacgccgt
     4441 gaagatgaat ggtcaaaccg taagccgtga tggtgcgcgt aatctcggtg aagtacgtac
     4501 cggggttgag gcgaaagtaa ataacaacct tagcctgtgg gggaatgtcg gtgtgcaact
     4561 aggtgataaa ggctatagcg atactcaggg catgctggga gtgaaatata gctggtaaac
     4621 cgtataagcc gcatgtcgag atggcatgcg gcttaatatt gccgacttca aaggttttaa
     4681 agcctccctt ttatccgtga tcgcggatat cgcagcgttt atcccgtaca gcggataaga
     4741 tgtgtttcca gattgacttt atcctcacta aaggataaaa ctgataatat ccccttaagc
     4801 ggataaattt gctgtggacg tatgacatga tgagctttca gaagatctat agcccaacgc
     4861 aattagcgaa tgcaatgaaa ctggttcgcc agcaaaatgg ctggacgcag agcgagctgg
     4921 cgaaaaaaat tggcattaag caggcgacaa tttccaattt cgaaaacaac cctgacaata
     4981 ccacgctcac gacatttttt aagattttac agtcgcttga actctcaatg acactatgcg
     5041 acacgaaaaa tgcctcgcca gaatcaacag aacagcaaga tctggagtgg taatgcctaa
     5101 acttgtcact tggatgaaca accagcgggt aggcgagtta acgaagttag ccaacggcgc
     5161 gcacaccttt aagtatgcac cggagtggtt agccagccgt tatgccagac cgttgtcact
     5221 ttcgctgcca ttgcagaggg ggaatatcac ctcagatgcc gtatttaact tcttcgataa
     5281 cctgttaccc gatagcccga ttgtacgtga ccggatcgtt aaacgttatc atgctaaatc
     5341 cagacaaccg tttgatttat tgtcagaaat agggcgagac agcgttggtg ccgtaacgtt
     5401 attacccgaa aatgaaacca taacgcgccc gataatggca tgggaaaagc ttactgaagc
     5461 caggcttgaa gaagtattaa cggcttataa agcagatatc cctctgggca tgattagaga
     5521 agaaaatgac tttcgcatct cggttgctgg cgcgcaggag aagacagcgc tgcttagaat
     5581 aggcaatgac tggtgcattc cgaaaggaat aacgccgacg acgcacatca ttaaattacc
     5641 gattggcgaa atcaggcagc ccaatgcgac gctcgatctc agccaaagcg ttgataatga
     5701 gtattactgt ctgctgctgg cgaaagaact tgggttgaat gttccggacg cagaaatcat
     5761 taaagcggga agggtgcgcg cgttagcggt cgaacgtttt gacaggcgtt ggaatactga
     5821 gcgaacggtt ttacttcgct tgccacagga ggatatgtgt cagacattcg gtttaccttc
     5881 atctgtgaaa tatgaatcag atggaggccc aggcatcgcg cggatcatgg cgtttttgat
     5941 ggggtccagc gaggcgcttc gcgatcgtta tgattttatg aaattccagg tcttccagtg
     6001 gttgattggc gcaacggatg gccatgcaaa aaacttctcc gtatttattc aggctggcgg
     6061 cagttatcga ctcacgccat tttacgacat catttcagca tttccggtcc ttggcggtac
     6121 gggaatacac atcagcgatc tcaaactggc aatggggctt aacgcatcca aaggcaaaaa
     6181 aacggcaatc gataaaattt atccgcgaca ttttttggcg acagcaaagg tgctgagatt
     6241 cccggaagtg cagatgcatg aaatcctaag tgactttgcc agaatgattc cggcagcact
     6301 ggataacgtg aagacttcat taccgacaga ttttccggag aacgtggtga cggcagttga
     6361 aaccaatgtg ttgaggttgc atggacggtt aagccgagaa tacggtagta agtgagatat
     6421 ggggtttggt cgttgttaat gagcacgaca atcatgaccg ccaacgactg gattcttcct
     6481 tggtttatac tggtgcgcat aatgtatatt atgttaaatc atgtctgagt gatgcgaagt
     6541 gcttttctgg tagtcgttat tcgttcaggg ttcaagaact taagcctctc ctctttcata
     6601 attggcctgt gcatggagaa atagcctggt tattccctgc cccatgctaa gagcgcagtg
     6661 tctgtcgcat agacactgca tacgaaaaca ccagagggtg ctgttactta tttaaggacg
     6721 gatcaagcgg gaatgagtcc actccggtat cagaaatggc tacgtctcaa tgaagtcagg
     6781 cgaccgatgc tgaatgaaca ttacgatgtc accacagcag cctatgctgt cggttacgaa
     6841 agctatccca tttcagtcgg gaatattcgc ggatgtttgg agagtcaccc aagagagata
     6901 ttaccaggtt aagaaagtct gtcggtcagc tttaataaac cctgcactta tctgtttaga
     6961 gcaattcggt gttagtttca gcaagcaaac attaaccata gctaatgatt tatagccata
     7021 ttaaccattg gtagcccttg acctttcctg acacttccct tgttcgcaat aatattctgt
     7081 ttagtcacgg tttataaata acatgcataa tgaaatcgtc atgttattta gcgacataaa
     7141 atgtttttgc aaatactctc ttcaacaagt gcacaataaa cttgttgtga ttaggtgtcc
     7201 ttgtgattta aagatattat cgttcttcgt gaaatgtaat aaccctggaa actatattct
     7261 cagggcaaca accaatcaag aatgattctt gtttttaagc aataacagaa tgacttaatt
     7321 gttaactctc tttattcagc tacttaaata taaattttgg agaattatga aactcaaaca
     7381 tgttggtatg attgtcgttt ctgtgttggc gatgtcgtct gctgcggtaa gcgcagccga
     7441 gggtgatgaa tcagtaacga ccactgttaa tggcggtgtt attcatttta aaggtgaagt
     7501 ggtaaatgcc gcttgtgcaa ttgattcaga atcaatgaac caaacggttg agctgggtca
     7561 ggttcgttct tctcgcctgg ctaaagcggg tgacctcagc tccgccgttg gcttcaatat
     7621 caagctgaat gattgtgata ccaatgtttc cagtaatgca gctgttgcat tcctgggtac
     7681 tactgtcacc agtaatgacg atacgttagc gctgcagagt tcagcggcag gctctgccca
     7741 aaatgtcggt attcaaattt tggaccgtac gggtgaggta ttaatacttg atggggccac
     7801 ttttagtgct aaaaccgact tgattgatgg cacgaatata ctgccattcc aggctcgtta
     7861 tattgctctc gggcagtccg tagctggtac tgcaaacgct gatgcgacct tcaaagttca
     7921 atatctataa ttcaatctta aggtggctat acgtcatctg aaattgaaac agggtgtgtg
     7981 tggcatggct acgcacaccc gagcaattta ccaatattca tctcattttt aatgagaaat
     8041 ggtttgtatt tggagcgcta tgaaaaatca cataatatct aaactatcaa taaacaacag
     8101 tagttaaact tactgttatt taaaatattt actttgtagc aaggttttcc tattaaaaga
     8161 gaaccaacat ttattacaaa cgcattaagt ctggaaaccc ggcgactcgc caatgtatta
     8221 gcaacattaa tcgattgtct gatgtaacgg ttttcccgct tatcctctta acacatggag
     8281 aatgcatcag atgcaaacta cgcgtacccc ctactctatt tcctttatgg cgacagtgtt
     8341 acttttattg ctctttgctt gccatagcac cgttgccaac gcagctgtcg cactgggtgc
     8401 gacgcgggta atttatccgg caaaccagaa acaggtttta ttgcctgtca cgaataatga
     8461 tcctgccagt gtctatctca ttcagtcatg gatagaaaat gcaggtgacc aaaaagacac
     8521 ccagtttgtc attaccccac cgcttttttc catgcaaggt aaaaaagaga atacgctgcg
     8581 gataatcaac gctactaatc atcagttacc aggcgaccgt gaaagcctgt tctgggtgaa
     8641 tgttaaagct attcctgcta tggaaaaaga ccagaagaat gagaatacat tacagctggc
     8701 aattattagc cgcataaaaa tgttttatcg ccccacgaac ctggcaatgg cacctgaaga
     8761 agcgcctgca atgcttcggt ttcgtcgcag cggtagcaaa ctcacgctta ttaatccaac
     8821 gccctatttc attaccgtaa caaatatgaa agcaggaaat agcaatctcc caaacaccat
     8881 ggtgccgcca aaaggtgaag tatcggtgga tatcacgcat gctgcaaccg gggatatcag
     8941 ttttcagacg atcaatgatt acggtgcatt aactccgcgt attaaggcaa ccatgcagta
     9001 agccagtgac ctaattaata ataaactcaa tcggaattaa taatgacagc cattcgtgcc
     9061 gcatttaagg catatcgtat gcatcaggtg ttaataatgc ctcgctttgc acggcttacc
     9121 attgcccttg gcttagcaac tgcggtattt cccgttgacg cagagtatta ttttaatcca
     9181 cggtttttaa gcaatgatct tgctgaatct gtcgatttat ccgcctttac taagggacgc
     9241 gaggcaccac ctggtacata tcgggtcgac atttatttga acgatgaatt tatgactagt
     9301 cgggatatta cttttattgc agatgataat aatgcagaac tcatcccatg cctgagtaca
     9361 gatcttcttg tcagtcttgg tataaaaaaa tcagcgttat tagacaataa agaacattcg
     9421 gcagaaaaac atgtgccgga caacagcgca tgcacaccac ttcaggatcg tttggccgac
     9481 gcatcaactg agtttgatgt tggtcagcaa catttgtcac tctccgttcc gcaaatttat
     9541 gttggcagaa tggctcgcgg ctatgtttct cctgatctgt gggaagaagg aataaatgct
     9601 gggcttctaa actatagttt taatggtaat tctattaata atcgtagtaa ccataatgca
     9661 ggaaaatcaa actatgcata tttgaattta cagagtggca tcaacattgg tagttggcga
     9721 ctacgcgata actcaacgtg gagttataac agtgggagta gcaattcatc tgacagcaat
     9781 aaatggcagc atatcaatac gtcggctgaa cgtgacatta ttcccttacg ctcacggtta
     9841 acggtaggtg atagttatac cgatggcgat atttttgata gtgtgaactt tcgtggcctc
     9901 aaaataaatt caacagaagc gatgttgccc gatagccaac atggtttcgc tcctgtgatt
     9961 catggtattg cccgtggaac cgcacaagtg agtgtaaaac agaacggata cgatgtttat
    10021 cagactactg ttccacccgg cccttttact attgatgata tcaactctgc gaccaatggt
    10081 ggtgatctgc aagtaaccat aaaagaggca gacggcagta ttcagacatt atatgttcct
    10141 tattcgtctg ttccggttct ccaacgtgct ggatatacgc gttatgcgct tgccatgggg
    10201 gaatatcgta gtggaaataa cctgcaaagc tctcccaagt tcatacaagg tagcttgatg
    10261 catggtctgg aaggaaactg gacaccttat ggcggaatgc aaattgcaga agattatcag
    10321 gccttcaacc ttggtattgg gaaagattta ggactttttg gtgccttttc tttcgatatc
    10381 acgcaggcca atacgacact tgcagatggc acccgtcaca gtgggcaatc ggttaaatcc
    10441 gtctacagca aatccttcta ccagacagga acaatatcca ggtcgcagga tatcgctatt
    10501 ctacgcaagg tttttataac ttatccgaca gtgcctacag tcgaatgagt ggttacaccg
    10561 tcaagcctcc taccggagac accaatgagc agacacaatt tattgattat tttaatctgt
    10621 tctacagtaa gcgtggtcag gaacaaataa gcatctctca gcagcttgga aattacggta
    10681 cgacattttt cagtgccagt cgccaaagtt actggaacac gtcacgcagc gaccagcaaa
    10741 tatcatttgg attaaatgtg ccgtttggtg atattacgac ttcgctgaat tacagctatt
    10801 ccaataatat atggcaaaac gatcgggatc atttactcgc ttttacgctt aatgttccct
    10861 tcagtcattg gatgcgtaca gacagtcagt cggcatttcg taattcaaac gccagttaca
    10921 gtatgtcaaa cgatttgaaa ggcggcatga ccaatctatc gggggtttat ggcactctgc
    10981 tgccggataa taacctgaat tatagcgttc aggtcggtaa cacccacgga ggtaatacat
    11041 cgtctggcac cagtggttac agttctctta attatcgtgg agcttacggt aatacgaatg
    11101 tcggttacag tcggaatggt gacagcagcc agatttatta cggaatgagt ggtgggatta
    11161 ttgctcatgc tgatggcatc acctttggac agccgctggg cgacacaatg gttctggtta
    11221 aggctcctgg cgctgataat gtcaaaatag agaaccagac cggaattcat accgactggc
    11281 gtggctatgc aatattacca tttgcgacag aatatagaga aaatcgtgtc gctcttaacg
    11341 cgaattccct tgcagataat gttgaactgg atgaaaccgt agtcactgtc atcccaactc
    11401 acggtgctat tgccagagca acatttaatg cacaaatcgg cgggaaagta ttaatgacgt
    11461 tgaagtacgg taataaaagc gttccattcg gtgcaattgt cactcacgga gagaataaaa
    11521 atggcagcat tgtcgcggaa aatggtcagg tttatctgac tggacttcca cagtcaggga
    11581 aattacaggt ttcatggggc aatgataaaa actcaaactg tattgtcgat tacaagcttc
    11641 ctgaagtctc tcctggaacc ttgctgaacc agcagacagc aatctgtcgc taaggaacaa
    11701 tagattatga aatacaataa cgttattttc ctcggtttat gtctggggtt aaccacctat
    11761 tctgctttat ccgcagatag cgttattaaa attagcgggc gcgtcctcga ttatggctgc
    11821 acagtctcat cggattcgct taattttacc gtagatctcc aaaaaaacag tgccagacaa
    11881 tttccaacga ccggtagcac aagtccagcc gtcccttttc agattacgtt aagtgaatgc
    11941 agcaaaggga caacgggggt tcgggttgca tttaacggta ttgaggatgc agaaaataat
    12001 actctgttga aactggatga gggaagcaat acggcctccg gtttaggtat agaaatactg
    12061 gacggaaata tgcgtccggt gaaactgaat gaccttcatg ccgggatgca gtggatccca
    12121 ctggtaccag aacagaacaa tattttgcct tactccgctc gtctgaagtc aactcagaag
    12181 tccgtcaatc cgggactggt gagggcttcg gcaaccttta cccttgaatt tcaataatca
    12241 gagtcagata tgaaaagact tcacaagagg ttcctgttag ctacgttttg cgcgttattc
    12301 acagcaactc tccaggccgc cgatgtcact atcactgtta atggtcgggt agtcgctaaa
    12361 ccctgcacta ttcaaaccaa agaagctaac gttaatctcg gggatcttta tacgcgcaat
    12421 ctgcaacaac ctggttctgc atctggctgg cacaatatta ctttgtcatt aaccgattgt
    12481 ccggttgaaa caagtgcagt gacggcaatc gtgacaggtt caactgacaa tacgggttat
    12541 tacaaaaatg aaggtactgc cgaaaatatt cagatagagc tgagggatga ccaggatgcg
    12601 acgttaaaaa atggcgatag caaaacggtt attgttgatg agatcactcg taatgcacag
    12661 tttccactta aggcaagagc tatcacggtg aatggaaacg caagccaggg aacgatcgag
    12721 gcgctaatca atgtgatcta cacctggcaa taacgtcgat tcgattttct tagccttctt
    12781 tttaaatcaa ttttttaacc ttaggatata tcatgggtaa aacaatcagt ataaaggtcc
    12841 tgttcggtat ttatcttcta ttaatggcag gtaaagtttt tgccttcagt tgtaatgtcg
    12901 atggcgggtc aagtattgga gctggtacaa cctctgttta tgttaatctc gaccctgtaa
    12961 tacagccggg ccagaatctg gttgtagact tgtctcagca tataagttgc tggaatgatt
    13021 acggcggctg gtacgacact gatcatataa acctggtaca aggctcagct ttcgcaggct
    13081 ctctacagtc atataaagga tcgctgtact ggaataatgt gacctacccc ttcccgttga
    13141 cgacaaatac caacgtattg gatattggtg ataagacacc gatgccatta ccgttaaaac
    13201 tctatattac cccagtcggg gcggccggtg gagttgtcat aaaagccggg gaagttattg
    13261 cccgtattca tatgtataaa attgcaacat taggaagcgg aaatccgcgt aattttacgt
    13321 ggaatattat ttcaaataac agtgttgtta tgccaacagg cggctgcact gttgatagtc
    13381 gtaatgtcac cgtggatctc ccggactttc ctggaagcgc agaaataccc cttggcgtat
    13441 attgctcaag cgaacaaaag ctctcatttt atctttcggg tgccaccact gacagtgccc
    13501 gacaagtgtt tgctaacaca gcacctgatg caacaaaagc atcgggagtg ggtgtctctc
    13561 tgatgcgtaa tggaaaaata ctggctacgg gagaaaatgt ctccctggga acagtaaata
    13621 aaagcaaagt acctctgggt ttaagcgcaa cctatggaca aacaggaaac aaggtttcag
    13681 ccggaaccgt ccagtcagtc attggtgtca cttttatcta tgaataattg tgattttgat
    13741 cttagtctat gcgctccatt cacgaaaatt gcgcggataa ttattttgta aaggctatta
    13801 tcctacacct gtaagaaaat ccgcgcacaa ttgaagccag agtcttattt tttatattga
    13861 aaaatatgtt aatttaaaca ctatgagaga tgatgcttaa cttatcgtat ttaatctatt
    13921 gtttaacgat aatagattaa aaatagaaag agagtagtcc cctactctta tatatccatg
    13981 ttggcgataa tccctttgta tatactgtgc atcatcgcta ttacaaatcc taataattca
    14041 tttccacaca ggataagtag atgaagaaaa aaattgaatc ctaccagggt gctgccggtg
    14101 gttggggtgc tgttaaatcc gtagcgaatg cagtacgtaa gcagatggat atacgccagg
    14161 atgttattgc catgtttgac atgaataagc cagagggctt tgactgtccg ggttgtgcat
    14221 ggccagatcc taagcacagt gcgtcattcg acatttgtga aaacggcgca aaagcaatcg
    14281 cctgggaagt cacggataag caggtaaatg cctctttctt tgctgagaat acggttcaat
    14341 cattacttac ctggggagat cacgagcttg aggctgcggg acgtctcact cagcctttga
    14401 aatatgatgc tgtcagcgac tgttacaagc cattaagctg gcaacaagct ttcgacgaaa
    14461 ttggcgcacg ccttcaaagc tatagtgatc ccaatcaggt tgaattctat acttcgggcc
    14521 gcacttccaa tgaagctgcc tttctttatc agctttttgc ccgtgaatac gggagtaata
    14581 actttcccga ctgctccaac atgtgccatg aaccgaccag tgtgggtttg gcagcgagta
    14641 tcggtgtggg taaagggact gtgttgctgg aagactttga gaagtgcgat ttagtcattt
    14701 gcattgggca taaccctggt acaaaccacc cccgcatgct gacttcgttg cgcgctttag
    14761 tgaaacgggg agcgaaaatg atcgccatca atcctctaca ggaacgtggc ctggagcgat
    14821 ttaccgcacc gcaaaacccg tttgaaatgc tgacgaactc tgagactcag ttggctagtg
    14881 cctactataa cgtgcgcatt ggtggcgata tggcgttgct caaggggatg atgcgcctgt
    14941 taattgagcg cgatgatgct gcaagtgccg caggtcggcc ctcattgctg gatgacgaat
    15001 ttattcaaac gcataccgtc ggctttgacg agctacgccg tgacgttctc aattccgagt
    15061 ggaaagatat cgaacgtatt tctggattaa gtcagactca aatcgccgaa ctggctgatg
    15121 catatgccgc tgccgaacga accattatct gttacggaat ggggatcact cagcacgaac
    15181 atggtaccca gaatgtacag caactggtca atctgctgtt gatgaaaggt aacattggca
    15241 agcctggtgc gggtatctgc ccactacgtg gccactctaa tgtacagggc gaccgaaccg
    15301 tcggtatcac cgagaaaccg tctgcagagt ttctggctcg cctgggtgag cgctatggct
    15361 tcacaccacc tcaagcacct ggacatgctg caattgccag catgcaagca atatgtacag
    15421 ggcaggctcg agcattgatc tgcatggggg gcaactttgc actggcaatg ccagatcggg
    15481 aagcgagcgc tgtaccgtta acgcaattag atctggcggt acacgtagcc actaagctta
    15541 accgctctca tctgctgacc gcacggcata gctatattct gccggttctg ggacgtagcg
    15601 agattgacat gcaaaaaagc ggtgcgcagg cggtaaccgt tgaggattca atgtcgatga
    15661 ttcatgcctc gcgtggcgtg ttaaaacccg ccggtgtaat gctgaaatca gagtgtgcag
    15721 tggtcgcggg aatcgcgcag gcagcactac cccagagcgt ggtagcctgg gagtatctgg
    15781 tggaagatta tgatcgcatt cgcaatgaca ttgaagctgt gctgccagag ttcgccgact
    15841 ataaccaacg catccgtcat cccggtggtt ttcacctgat aaatgcagct gctgaaaggc
    15901 gctggatgac gccgtcaggt aaggctaatt tcattaccag caaagggctg ttagaagatc
    15961 cctcttcagc gtttaacagt aagctggtca tggcgacagt acgcagccac gatcagtaca
    16021 acacgacgat ttatggtatg gatgatcgct atcgaggggt attcggtcaa cgagatgtgg
    16081 tctttatgag tgctaaacaa gctaaaattt gccgtgtaaa aaacggcgaa agagttaatc
    16141 ttattgcgct cacgccagac ggtaaacgta gctcacgccg catggataga ttaaaagtgg
    16201 tcatttaccc tatggctgac cgctcactgg tgacctattt tccagaatcg aatcacatgc
    16261 taacactcga taaccacgat ccattaagtg gcattcctgg ctataaaagt attccggttg
    16321 aattagaacc atcaaattaa tgcctcttct catttcttct gctgtcatcc gcacagcaga
    16381 agaatccctc attgactatt atcgcgcaat ttgctcacat ggattaaatt aaactacata
    16441 ctataagata taaacctctg cctacagctg taagaaactc cgctcagtac tgaagtgcca
    16501 gtcctatttc ctcttttctc cagcctgtta tattaagcat actgattaac gatttttaac
    16561 gttatccgct aaataaacat atttgaaatg catgcgacca cagtgaaaaa caaaatcacg
    16621 caaagagaca actataaaga aatcatgtct gtaattgtgg ttgtcttatt actgaccctt
    16681 acgttgatag ccattttttc ggcaattgat cagctgggta tttcagaaat gggtcgcatt
    16741 gcaagagatc ttacacattt cattatcaat agtttgcaag actgataata aacagcaaat
    16801 tataaatctg aaatgttaac aaagtatcga taaaaacttt attgttttaa ggagataaaa
    16861 tgtcgctcgt ttgttctgtc atatttattc atcatgcttt caacgctaac attttagata
    16921 aagattacgc cttctctgac ggcgagatcc tgatggtaga taacgctgtt cgtacacatt
    16981 ttgaacctta tgagcggcat tttaaagaga tcggatttac tgaaaatacc attaaaaaat
    17041 atctacaatg cactaacatc cagacagtga cgatgcctgt tcctgcgaag tttttacgtg
    17101 cttcaaatgt accgactgga ttgcttaatg aaatgattgc ttatctcaac tcggaagaac
    17161 gcaatcatca taatttttca gaacttttgc ttttttcttg cctgtctatt tttgccgcat
    17221 gcaaaggttt cattacacta ttaactaacg gtgtgctatc cgtttccggg aaagtgagaa
    17281 atattgtcaa catgaagctg gcgcacccat ggaagctgaa agatatttgt gactgcctgt
    17341 acatcagtga aagcctgttg aagaaaaaac taaagcaaga gcaaacgaca ttctcacaga
    17401 ttctattaga tgccagaatg cagcacgcaa aaaatttgat acgcgtagaa ggttcagtca
    17461 ataaaattgc cgaacaatgt ggttatgcca gtacatctta ttttatttat gcgttccgca
    17521 aacatttcgg caacagtccg aagagagttt ctaaggagta ccgttgtcaa agtcacatgg
    17581 gtatgaatac gggcaacacg atgaatgctt tagctatttg attatttgct aacgagtagt
    17641 caaccacgca ccctacgtaa aaattaaaaa tggggcagtc attccctgcc ccgcgttgtt
    17701 tttaggtgat atatttattg aaataaataa gtgacattca tcacatattt atgcatttgc
    17761 ataacctgtt gcatgattat ttatgatctc aattcggcat tttgtcagtg aaatgcaata
    17821 atttattaaa tatcaataga ttagttgttt atcggcgaga aattacttag tagaacagaa
    17881 agtaatgtca acgctttatg gactgttatt ttcctttttt tagctaaatc tgctatctct
    17941 ttat
//
LOCUS       DAZGSP010000102        17831 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZGSP010000102 DAZGSP010000000
VERSION     DAZGSP010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.103"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..640
                     /locus_tag="ACHK74_003897"
     CDS             <1..640
                     /locus_tag="ACHK74_003897"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP0222633.1"
                     /translation="AYLIAAGLSDYQLTEKDYLILEHFKNIEFSGFPVTELFSVCNTG
                     NILSRDIIENSGDVPYLCASRDNNSVSSYIAYDDSLLEKGNCIFIGGKTFVVTYQERD
                     FFSNDSHNLRLYVNNEDGRTKFAYLGIISCIYRSISHKYSWGDSVSNRKIKNDVIWLP
                     VKNKSPDFCYINDLISVVHKLVIKDVVQYAERKMNAYRQAITSRGYDDSGVS"
     gene            1413..3350
                     /locus_tag="ACHK74_003898"
     CDS             1413..3350
                     /locus_tag="ACHK74_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP0222634.1"
                     /translation="MAFKHYDVVRAASPSDLAKRITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVASEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAVCKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPVNHAAQFGALVDKFRADLAD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLATAIL
                     RHAGRVALNAGASSTVSEVRPSSPSGAEATGVTTLLSYLASESEGSLKVQGWSASGGR
                     AEVVSDAEGTGGKAVKLTKEAGKSSWVLEYAAGNGAALLQKGGQIRCRFKVSGALAAN
                     QYVMAFYWPVSSLPQGVALTGDGGNNLLAAFYIQTDAKDLNVMYHNAKVATNNLKLGT
                     FGAFDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKLHVTDITRN
                     ATYPVLIDSIAVEVNSTDTAA"
     gene            3486..3665
                     /locus_tag="ACHK74_003899"
     CDS             3486..3665
                     /locus_tag="ACHK74_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0222635.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAVRDCWRRQIDKRAAEKI
                     SAGQSAGSKPEEPLI"
     gene            3706..3951
                     /locus_tag="ACHK74_003900"
     CDS             3706..3951
                     /locus_tag="ACHK74_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP0222636.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            4029..4244
                     /locus_tag="ACHK74_003901"
     CDS             4029..4244
                     /locus_tag="ACHK74_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP0222637.1"
                     /translation="MNQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            4249..4782
                     /locus_tag="ACHK74_003902"
     CDS             4249..4782
                     /locus_tag="ACHK74_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0222638.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDHVNAIERDKALAWVEKNIRVPLTDPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            5004..5112
                     /locus_tag="ACHK74_003903"
                     /pseudo
     CDS             5004..5112
                     /locus_tag="ACHK74_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5334..5519
                     /locus_tag="ACHK74_003904"
     CDS             5334..5519
                     /locus_tag="ACHK74_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP0222639.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPG
                     PDWQTPLNGIISSSENG"
     gene            5876..6682
                     /locus_tag="ACHK74_003905"
     CDS             5876..6682
                     /locus_tag="ACHK74_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP0222640.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPCNDAGSGDEKCAKNSEKNCANSAETKRNSRSGNP
                     HPVARFSDRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTSQRRGVITPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            6663..8369
                     /locus_tag="ACHK74_003906"
     CDS             6663..8369
                     /locus_tag="ACHK74_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP0222641.1"
                     /translation="MTFRKNKTRCDEPAEMTETEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRNMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQESTASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYISKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            8369..10513
                     /locus_tag="ACHK74_003907"
     CDS             8369..10513
                     /locus_tag="ACHK74_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP0222642.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFRVST
                     VSRNEVFWDWLSRESDLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TITEGQPSPLMSAWEEYQSWDRRENEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDSDLMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            10671..11678
                     /locus_tag="ACHK74_003908"
     CDS             10671..11678
                     /locus_tag="ACHK74_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222643.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSTQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNQADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGIGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            11702..12916
                     /locus_tag="ACHK74_003909"
     CDS             11702..12916
                     /locus_tag="ACHK74_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFP0222644.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            12972..13361
                     /locus_tag="ACHK74_003910"
     CDS             12972..13361
                     /locus_tag="ACHK74_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222645.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHVLIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            13411..13872
                     /locus_tag="ACHK74_003911"
     CDS             13411..13872
                     /locus_tag="ACHK74_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222646.1"
                     /translation="MSEKIAVVYIGPKTVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERVLAARAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            13856..14419
                     /locus_tag="ACHK74_003912"
     CDS             13856..14419
                     /locus_tag="ACHK74_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222647.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPLRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFY"
     gene            14419..15069
                     /locus_tag="ACHK74_003913"
     CDS             14419..15069
                     /locus_tag="ACHK74_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFP0222648.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPECFISNDLSPRVFWLFPVSDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            15066..16862
                     /locus_tag="ACHK74_003914"
     CDS             15066..16862
                     /locus_tag="ACHK74_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFP0222649.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKASEAAEAATRAGESEKAAAADAKNAKQHAETARVAQQAAGDILERAEAATVSA
                     EEARRMAENARGPQGDQGPKGDTGPRGEKGEPGQRGPQGPKGERGERGPQGSVGPAGP
                     RGEKGEQGERGPQGLQGLKGETGERGAQGAQGPAGPTGATGPAGPRGETGPQGAQGPA
                     GATGPVGPQGERGIQGPAGPAGPRGETGARGEKGDPGDPGGPPGPKGDTGPAGPAGEK
                     GEPGERGPQGLQGPPGPAGERGPAGEPGAKGETGKPGPAGPQGQPGPQGLKGDRGEPG
                     PTGPQGPAGERGPQGPRGVAGPVGPAGAAGAKGDAGPAGPAGPRGPAGERGPAGPKGE
                     RGEPGPMGPPGPAGDAGNSTVKGIRLGNEMVYRPYSGYSNLSLRSMDGCVLTGIAGTN
                     MPDKSRIDEIYYRPLQMTFGDGSWRSVGIGEV"
     gene            17134..17799
                     /locus_tag="ACHK74_003915"
     CDS             17134..17799
                     /locus_tag="ACHK74_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFP0222650.1"
                     /translation="MIIKSFKLYTPEKSDVPGAMYLKSVDGRDWYESQSLFAADTLKL
                     VYDSNGIITSISKDVSMLWPVNQSVAEVADTEENRKADISGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNEMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVPVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRV
                     DWK"
BASE COUNT         4613 a         4085 c         5186 g         3947 t
ORIGIN      
        1 agcatacctg atagctgctg ggctgagtga ttaccaacta acggaaaaag attatcttat
       61 cctggaacat tttaaaaata ttgaattttc aggctttcct gttactgagt tattctcagt
      121 gtgcaataca ggaaacatat tgtctagaga catcatagaa aatagtgggg atgttcctta
      181 tctttgtgct agccgtgata ataactctgt tagttcttat atcgcctatg atgactcact
      241 cttggaaaaa gggaactgta tttttattgg tggtaagaca ttcgttgtaa cttatcagga
      301 gcgagacttt ttttcaaacg acagtcataa tttgcgactc tatgttaaca atgaggatgg
      361 aaggacaaag tttgcttatt tgggcattat ttcatgtatt tataggtcta tttcacataa
      421 atattcatgg ggggatagtg ttagtaatag aaaaattaag aacgatgtta tttggttgcc
      481 agtgaaaaat aagtcacctg acttttgtta tataaatgac cttatttcag ttgttcataa
      541 gcttgtcatt aaagatgttg tccagtacgc agaacgcaaa atgaacgcat acaggcaagc
      601 tataacaagt cgcggttatg atgattctgg tgtcagttaa aaaacatttt acaatcgtaa
      661 aaatcctcat atcatgataa gaatggttac attgccacgc tgcttaaccc gccgatgcgc
      721 gggttttttg tatccggaat tctgtgggtt accctgcccc ccttagaaag gtgcgacagc
      781 aattaataac aaaatcttaa aaatcgcata tggcactatt agttttctaa atattgtata
      841 ttttaagtat tgcaggataa ccctgtaacg aagtttgcgt aacagcattt tgctctacga
      901 gtttgccagc ctcccccagt ggctggcttt ttttgtatcc gttcaacagg aatgttacat
      961 acctcacaat taagtcagtt gaatgttgtc tgcccggatg agaatttgtt agaaaaaaac
     1021 tgcatggtga atccccctga gcggaggggc gactggtgac ggtataatct ctgattatca
     1081 aaacgagaat gacgagggtt tagtggcacc gggctgaact caccgggagg cacccggcac
     1141 catgcaatgg cacatagcgc cactctccag cccctctccg gaggggcttt cttatggaca
     1201 aaaaagcccg cgctgggaga cgcgggcggc aaggaataaa caatgaaacg tgaagtaata
     1261 tttcagctgg cgaataatac cctatagtaa tcactctgcg caaccttttt cgaattgcgg
     1321 gctgtagtct cccttctgcc attgtcctgt aacttccgga cttcagcccg ctccttattt
     1381 tactcacaat attatcccgg ccgggaggat tcatggcatt taaacactat gatgttgtca
     1441 gggcggcgtc gccgtcagac cttgcgaaac gaataactca aaaactgaag gaagggtggc
     1501 agccttatgg tagtgcgctg atttcgacag ctggttatgg tgcggagttc atccagccag
     1561 ttgtgagtga ggggagcatc tcatcaccag aggagccagg caaccgtccg acgacctcag
     1621 cgccttctgt tgcgtcagaa tattactatg tgatcgcgct tgctggtcag tccaatggta
     1681 tgtcatacgg tgagggactg ccattgccgg atacattcga cagccctgat ccacgtatta
     1741 aacagttagc gcgtcgcagt acggtgacac cgggcggtgc agtatgcaaa tataacgaca
     1801 tcattccggc ggaccattgt ctgcatgatg tgcaggacat gagccgcctt aaccatccga
     1861 aagcggacct gtcaaagggg cagtacggta ccgtggggca ggggctgcat atcgccaaaa
     1921 aactgctgcc gtttataccg gcgaatgcgg gcattctgct ggttccgtgc tgtcgtggtg
     1981 gttcagcgtt caccaccgga gctgatggca catacagtga cgcgagtggt gcttcggaga
     2041 attcaacccg ctggggtgtg gacaagccgc tgtataagga ccttatcggt cgaacaaaag
     2101 cagcactgaa gaagaacccg aaaaatgtgc tgtttgccgt ggtgtggatg cagggggaat
     2161 ttgattttgg cggtacgccg gtaaatcatg ccgcacagtt tggtgcgctg gttgataaat
     2221 tccgtgcaga cttggcggat atggcaggcc agtgcgtcgg tggctctgct ggcggtgttc
     2281 cctggatatg cggggacacg acgtatttct ggaagcagaa gaacgaatcc acgtaccaga
     2341 cggtgtatgg cagctataaa aacaaaacgg aaaagaatat ccatttcgta ccgttcatga
     2401 cggatgagaa cggggtgaat gtgccgacga acaaaccgga agaagacccg gacattccgg
     2461 gtatcggata ttacggttcg aaatggcgtg acagctcagc cacctggacg tcacaggaca
     2521 gggcgagcca tttcagttca tgggctcgcc gcgggattat ttccgaccgt ctggcaacgg
     2581 cgattttgcg ccatgcggga agagtggcgc taaacgcggg ggcatcatcg acagtatcag
     2641 aggtgcgccc gtcatcgcct tccggtgcag aagccacagg cgtcacaaca ctgctctctt
     2701 accttgccag cgagtcagag ggaagcctga aagtacaggg atggtcagcc agtggcggca
     2761 gggcagaagt ggtcagcgat gcggagggaa ccggaggtaa ggcagtgaag ctgaccaagg
     2821 aggccggtaa aagcagctgg gtgctggagt acgccgcggg caacggtgcg gctctgttac
     2881 agaaaggggg gcagattcgc tgccgcttta aggtttcggg agcgctggct gcgaaccagt
     2941 atgttatggc gttttactgg ccggtctctt cactgccaca gggcgttgcc ctgaccggag
     3001 acggggggaa taacctgctg gcagcgttct acatccagac agatgcaaaa gacctgaatg
     3061 tgatgtacca caatgcgaaa gtggcgacaa acaacctgaa actgggaacc tttggcgcat
     3121 ttgataacga atggcatacg ctggctttcc gctttgccgg gaataacagc cttcaggtga
     3181 cgccggttat tgatggtcag gatggcacac cgttcacgct gacgcagtca ccggtcagtg
     3241 catttgcggc ggataaactg catgtgacag acattaccag gaatgcgact tacccggtac
     3301 tgatagacag cattgcggtg gaagtgaaca gcacagacac tgcggcatga taaaaaaaac
     3361 cgccagcgac aggaatggac gctggcggtg gtgataccta tggagaaaaa ataaaggaac
     3421 gatactttcg tactctggtt tttaatgaaa acagttctta ttgtcaacaa taacggaaag
     3481 aaattatgac atttctgaac cagttaatgc tgtacttctg tacggtggtc tgtgtgctgt
     3541 atctcctttc gggtgggtac agggcagtgc gcgattgctg gcgcaggcag attgacaaaa
     3601 gggccgctga gaaaatcagc gccggtcagt cagccggaag caaacccgaa gagccgctca
     3661 tttagcggca actttcttaa tcacaccttt cgacgagaaa atcccatgtc agaaattaca
     3721 tccctggtca ctgcagaggc agtgaaggaa gtcctgcgct ctgaagaagt ccggagcgca
     3781 ctgaaacaga aacttcgcca taacctggaa gcgcgtcttg atgcagaagt ggatgccatt
     3841 ctggatgaac tgctgggcgc accggcagct ccggagccgg aaggcatcgc gggtgagggg
     3901 agtgcttcag atagcggtga ccccacaccg gacagcgaca tgatgatgta agcatgcgtc
     3961 agggaccatc ggtgtgtgcc ggtggtcttt tttattgttg tgagcttccg gattgcggga
     4021 ggcggggtat gaaccagatg gaaaaaatca caacaggtgt gtcatacacc acgtcagcgg
     4081 tgggaacggg ctactggttc ctgcagttgc tggacagggt ttccccgtct cagtgggcgg
     4141 caataggcgt gctggggagt ctgctgtttg gtctgctgac gtacctgacg aacctgtatt
     4201 tcaaaatcag agaggacagg cgtaaggcgg cacggggaga gtaagctgat gagcaggaaa
     4261 ctccgctatg gtttatcggc tgccgttctg gcgctgattg ccgcaggtgc ttctgcgcct
     4321 gaaatcctcg accagtttct ggatgaaaag gaaggtaacc acaccacggc ataccgtgat
     4381 ggtgcgggta tctggaccat ctgccgtgga gccacccggg tggatggtaa gcctgtgatt
     4441 cctggcatga agctgtcgaa ggaaaaatgc gaccatgtta acgccattga acgtgataag
     4501 gcgctggcat gggtggagaa aaacatcaga gtgccgctga ccgaccccca gaaagcgggg
     4561 atagcgtcat tctgtccgta taacattggc cccggtaagt gtttcccgtc gacgttttat
     4621 aaacgaatta atgcaggtga tcgaaaaggt gcctgcgaag cgattcgctg gtggattaag
     4681 gacggtggca gagactgccg tatccgctca aataattgct acggtcaggt atcccggcga
     4741 gatcaggaaa gtgcgctggc gtgctgggga attgacagat aagcagaata ttttgctgaa
     4801 aaatgacatt ggccaacgcg ggcggataac acgaaatcct gcgaactggc aaaacctaag
     4861 tgaataaaag taaaaacccc ggttgttggc agcaagcggg gtttggtgtt ttctgacctt
     4921 gaataaggca agggagaaat tatgggtagg gaggtacttt ccctgtgagg aagtataaaa
     4981 gattctttct gaggttgtcc attatgaaag gcattgaagt ggagagccag tctggattta
     5041 acaagagcgg cagcttttgc cattcgtatt gtggccattg ctgttctggt ctgggcaatc
     5101 cgttggtggt gatatgaacc gtgttctgtg cgtggtcatc attgtcctgc tggtggcctg
     5161 tggtgcgctt agtctggggc tgaatcatta ccgtgttcac gccatcatct acaaagagca
     5221 gcgcgataaa aaagccagtg agctggagct ggcgaacgcg acaattactg atatgcagat
     5281 acgccagcgt gatgtcgctg cacttgatgc cagatactcg agggaattag ccgatgcgag
     5341 agctgaaaat gaaactctgc gtgctgatgt tgccgctggt cgtaagcgcc tgcggatcaa
     5401 caccacctgc tccggtaccg tgcgtgaagc caccggcacc tccggcgtgg ataatgcaac
     5461 caggccccga ctggcagaca ccgctgaacg ggattatttc atcctcagag aacggttgat
     5521 gacaatgcag aagcagctgg aaggggcaca ggactatatc cgcactcagt gcctgaaata
     5581 agttttgctg atgcgccgta tcgtcgctgt attccctcat taacagagac tgcagccacc
     5641 ttatctgcgt gagtgtgcgg gaataatcaa aaacgatgca caccgggttt tctcatttgt
     5701 cacgagatgt gagcgattcc ccgcgaagct gcccgtccgg tgcggtggtg gaagaaaccg
     5761 gataaaacaa ctgctttgaa caaatattgg tcaaataagg cgctgctgtg tgaattctga
     5821 aaaatcacag cggtcattat gcgtcagttt ttaacagagg acgtcagaaa gtgacatggc
     5881 aaagctggac tggaaaaagc tggagcaggc attccgacgc gaacatgccg aaacgggaat
     5941 aacattactg gactggtgcc ggaagaaaaa gattaattac aacaccgcca gaacccgtat
     6001 aaaaatgggc aaaatcgatc atgaaattga tcataaaacc gatcatgaaa tcgatcatga
     6061 catctcagat gaagaaccct gcaatgacgc gggttccggc gatgaaaaat gcgccaaaaa
     6121 ctctgaaaaa aactgcgcaa attcggcaga aacgaaacgc aacagcagat cgggcaatcc
     6181 tcaccccgtt gcccggttta gcgaccgtaa cacccacgcc gtaagacacc gtggatatgc
     6241 gaagtatctt gaggcagata acctcatgga tgatgcgtcc gacatggtgc tgttcgatga
     6301 actggtgttc acccgggccc gcgcactttc agtaactaag gcacttaaag ggatgttcgc
     6361 cgacctggaa gaggcaactg acgtggaaac ccgcgttgct ctttacgaca aaatactcaa
     6421 agctgaacag gcccttgacc ggaatattgc ccgtatcgag tcaattgaac gctcattgct
     6481 gacgctggac gtcctggctg agacagcacc aaaacttcgt gctgaccggg aaagaatcaa
     6541 cgccgccaga gataaactca gagcggaaac cgatattctg accagccagc gccggggtgt
     6601 tattacgcca gtcagtgaca tcgtgtcatc gctgcatgaa atgagtaatt cggggagact
     6661 ggatgacatt ccggaagaat aaaacgcgat gtgatgaacc tgcagaaatg actgagaccg
     6721 aacaacgtct gttcattatg acaaaactga gcaatccctg gtggcgcctc aatcatctct
     6781 acaaaataca gaacgaaaaa ggtgaactgg tcaccttcag aatgcgaccg gcgcaacgtc
     6841 agttgtttcg taacatgcat aataaaaaca ttatcctgaa agcgcgccag ctgggatttt
     6901 ccacagccat tgatatttat cttctcgacc aggcattatt cattccgcat ctcaaatgcg
     6961 ggatcgtcgc tcaggataaa caggctgcca gtgaaatttt ccgcacaaaa attgctgtac
     7021 cgtttgatca tctccctgac tggctgagag cctcattcac catcgttgaa cgtcgtagcg
     7081 gtgccagcgg tggctatatc ctgtttggtc acggctcgag tattcaggtg gcaacctcat
     7141 ttcgctcagg tacggtgcag cgcctgcata tctcagagca cggcaaaatt tgcgcgaaat
     7201 atccggctaa ggcgaaagag ctgcgaaccg gtacgcttaa tgccgtctct gatgaatgca
     7261 ttatttttga tgaatccacg gctgaaggcg tgggtggtga tttttacgag atgagtaacc
     7321 gtgcacagga gagcactgca tcaggcttat tgctgacggc acaggattat aaattccatt
     7381 tttacgcatg gtggcaggat cctaaataca gcgccagagt gcctgaaagc gggctgaagc
     7441 tgtcacggga aaaaatgacg tatttttctg cggttgagaa ggcaatgaac atcacgctta
     7501 ccgatgagca gaagcagtgg tacatcagta aggaaactga acagcgtgag gaaatgaagc
     7561 aggagtttcc ctcaacgcca caggaggcgt ttctgacgtc cggacgacgt gtgttcagtg
     7621 ccgaaagtac attgcaggca gaatcattct gttcgccacc gatgattgtt tatgacattg
     7681 aacctgttac aggagcgaag actaaagctc agtctctgcg tgaaggaaat aaaaacgagt
     7741 tgcagcggac gctgatgaat tatctgctgg tatgggaact gccggacccg gatgaagagt
     7801 atgtttgtgg ggcagatact gccgaagggc tggagcacgg agaccgctca tcgctggatg
     7861 ttgtcaaacg cagtaatggc gagcaggtgg ctcactggtt cgggcatctc gatgctgaac
     7921 tttttgctca tctcatttcg caggtctgtc gtatgtataa caacgcgttt gtggggccgg
     7981 agcgtaataa tcacggacat gcagttatcc tgaaactccg ggaactctat ccgacacgtt
     8041 atatctacaa cgaacagcat cttgaccagg catatgacga cgatacgccc cgccttggct
     8101 ggctgacaac ccgtcagagc aaacctgttc tgaccgaagg aatgaaaacg ctcctgaata
     8161 atggaatatc agggatccgc tggtcaggca cattatcgga aatgaacacc tacgtttatg
     8221 acgcgaaagg ctccatgaat gcacaggaag gctgctttga tgatcagctc atgagctaca
     8281 tgattgccca ggagatgcgc gccagaatgc cggtgagggt aaaacagaaa acggataaac
     8341 gcagaaccac acactggatg gcacactgat gaaaaatgaa attaacacca cagcgatgaa
     8401 aaacgatcat ggatccacgc cgcgtttttc tcagcgtcag ttactgtctc tctgttctga
     8461 tattgacagt cagcctctct ggcgtgatgc cgcaaacaag gcctgtgcgt attatgatgg
     8521 cgaccagctg gcaccggaag ttatccaggt actgaaagat cgcggtcagc ccatgaccat
     8581 ccataacctc attgccccca cggtagatgg tgtactggga atggaggcaa aaacaagaac
     8641 ggacctgata gtgatgtcag acgatccgaa cgatgaaaca gagaaactgg cagaggccat
     8701 taatgcggag tttgctgatg cgtgccgtct tggcaacatg aataaggccc gctccgatgc
     8761 ctatgcggaa caaatcaagg cggggctcag ttgggtggag gtcaggcgca acagcgatcc
     8821 gttcggacct gaattcaggg tatccacagt cagcagaaat gaggttttct gggactggct
     8881 cagccgggaa tccgacctga gtgactgccg gtggctgatg cgtcgccgct ggatggatac
     8941 cgatgaggca aaagccacgt tcccgggaat ggctcaggtt atcgattatg ctattgatga
     9001 ctggcgtggt tttgtcgata ccacgattac tgaaggccag cccagtccgt tgatgagtgc
     9061 atgggaagag tatcagtcat gggatcgcag ggagaatgaa tggcttcagc gtgaacgccg
     9121 tcgtgtgctg cttcaggtgg tttattaccg tacattcgag cgtcttccgg tgattgaact
     9181 cagtaatgga cgggtggtgg cttttgataa aaataatctg atgcaggcgg tggctgtggc
     9241 atccgggcgg gttcaggtga aagtcgggcg ggtaagtcgt attcgtgaag cctggtttgt
     9301 gggcccacac tttattgtgg atcgcccctg tagtgcaccg caggggatgt ttccgctggt
     9361 tcctttctgg ggataccgaa aggataaaac cggggagcca tacgggttaa tttcccgcgc
     9421 cattccggca caggatgagg tgaattttcg tcgtatcaag ctgacctggc tgcttcaggc
     9481 caaacgcgtg attatggacg aggatgccac ccagttgtca gacagcgacc tgatggagca
     9541 gatcgaacgt ccggatggca ttattaaact gaatccggcc cgaaaaaatc agaaaagtgt
     9601 cgcagatgtt tttcgggttg agcaggattt tcaggttgcc agccagcagt ttcaggtcat
     9661 gcaggaatcg gaaaaactta tccaggatac catgggagtg tattccgcat ttctcgggca
     9721 ggattcaggt gcgacgtcag gcgtggctat cagtaacctg gtggagcagg gggccacaac
     9781 ccttgcggaa atcaacgata actaccagtt tgcctgccag caggtgggaa gactgttgct
     9841 ggcttatctt ctcgatgacc tgaaaaaacg ccgtaatcat gcagtggtga ttaatcgcga
     9901 tgatcgccag cgtcgccaga ccattgtcct caatgctgaa ggtgataatg gtgaactgac
     9961 caatgatatt tcaaggttaa atacacatat tgcgctggcg cctgttcagc agacaccggc
    10021 gtttaaggca cagcttgcac agagaatgtc agaggttatt caggggctgc cgcctcaggt
    10081 acaggctgtt gtgctcgacc tgtgggttaa tcttctggat gtgccgcaga aacaggagtt
    10141 tgttgagcgt attcgtgctg cgctggggac gccaaaatca ccggatgaaa tgacgccgga
    10201 agaacaggaa gtagcggcac aacaacaggc acttcagcaa caacaggcag aactccagat
    10261 gcgcgagatg gctggcagag tggcaaaact ggaagctgac gccgccaggg cacatgcagc
    10321 tgcacaacgg gataatgcca gtgcacagcg ggaagtcgcc ctgacacagg ggcagcgtta
    10381 tgtggatgcg cttaaccagg cacatacggc agaaatcatt accggcgtac agaatatgga
    10441 acaggagcag gacgttcttc agcaacagat gctgtatacg ttacaacagc ggatgaatga
    10501 aatgtcgctc tgaaaactct ggcttcaact gaaccccgtc atcgtacggg gttttttgtt
    10561 tccggaggta agcgttccgg gagcggtgcg cttattcgcg ggggcagcga taagccttat
    10621 ttactcaacc attcggatct gtccgataaa cagaccatgc ggagttattt atggattttg
    10681 aatttacggg tgaagaaacc ccggaacaac tggaaaaaat gctggaagga cttggggatg
    10741 tggatattga cagtcacgca caggacgtcg tgacggaaga taccacggaa aaacatgcgg
    10801 atgaggaagc acagactcag acgggcgata acaatgtggc accgacgccg gatgccagtg
    10861 tggagcagac gcaggacgtg aaggagccgg aagcgaaggg ggtgctcacc cgcgacggta
    10921 aacacgtcat tccctatgaa gtccttgagg ctgaacgttc cggtaagcaa cgggccgaac
    10981 aggaagccgc acttcttcgt gggcagatag ctgaagaaaa acgcagggtg gaactactga
    11041 cgtctcagat ccaccaggcc ggtatgaagc ccacaccgtt accggaaaac gaaaaaattt
    11101 ctgatgagca gattgcccgt atcagggaga tgtatccgga aattggtgac gcggtggctt
    11161 cgctcatccg taaaaataac tatctccagt cccgtgttca gcaatcaaca cagcaggcag
    11221 aaggtaatgg tggtgaggat ttatcaccgg ttcttgatgc gatgaatgcc gtgccggtgc
    11281 tgaaaacgtg gcaggagtcc gatccggatc gcttctcggt tgctgtatcc atcgacggga
    11341 agctccagaa tgaccccgca tggaaagaca aaacgctcac tgaacgtttc gctgaagtgg
    11401 cccgtcgtac gcaggttgct ttcggtgaag tcagtgagtc gtctgctgac aaccaggcag
    11461 acaaaacgga tatccggaaa acggcggaag agaaagtgaa gaccgctgaa caggagcagg
    11521 cagtacctgc ttccccgtca gatttaggca ccacggcctc cgtcggaatc ggtgataatt
    11581 ttgaacggtt acttggtgct tctcattcag aggctgaggc gattatgcgt ggtatgacga
    11641 atgctgaaat agacgcactt ctggagaagc tcgggtaact tactgaagga gtactgaagt
    11701 aatgacgact gtaacatcag cccaggcgaa taagctgtat caggtggcgc tttttactgc
    11761 tgccaaccgc aaccgctcga tggttaatat ccttactgaa cagcaggaag cgccaaaagc
    11821 ggtttcgccg gacaagaaaa gcacgaagca gaccagcgca ggtgcgccgg ttgtccgtat
    11881 cacagacctt aacaaacagg ccggtgatga agtgaccttc agcatcatgc acaaactctc
    11941 aaaacgtccg acgatgggag atgagcgtgt tgaaggtcgt ggtgaggatc tcagccatgc
    12001 tgacttctcc ctgaaaatca atcagggacg tcacctggtg gatgcaggcg gacgtatgag
    12061 tcagcagcgc acgaagttta acctggcatc ctctgccaga acgcttctgg ggacgtactt
    12121 taatgacctg caggaccagt gtgcgatagt gcatcttgct ggagctcgtg gtgattttgt
    12181 tgctgacgac actattctgc cgacagcgga gcaccctgaa ttcaaaaaaa tcatgatcaa
    12241 cgatgtactg cctccgacac atgaccgtca cttttttggc ggtgatgcga caagctttga
    12301 gcagattgaa gcggcagata ttttttctat tggcctggtg gacaatctct ccctgttcat
    12361 tgacgaaatg gcgcatccat tacagccggt tcgtctgtcc ggtgatgaac ttcacggaga
    12421 agatccatat tacgtcctgt acgtcacgcc gcgtcagtgg aatgactggt acacctcgac
    12481 gtccggtaag gactggaacc agatgatggt tcgtgccgtg aaccgtgcaa aaggttttaa
    12541 tcatccgctg ttcaaaggtg aatgtgcaat gtggcgcaat atcctggttc gtaagtatgc
    12601 gggtatgccg atccgtttct atcaggggtc aaaggttctg gtatcagaga ataacctgac
    12661 ggcaaccacg aaagaggtcg ctgctgcaac caatattgac cgcgccatgt tactgggggc
    12721 tcaggcgctg gcaaatgctt acggtcagaa ggcgggcggt cacttcaaca tggttgagaa
    12781 gaaaacggat atggataacc gtactgagat agcaatcagc tggatcaacg gtctgaaaaa
    12841 aatccgtttc cccgagaaga gcggcaagat gcaggatcac ggcgtgattg ccgttgatac
    12901 agcagtgaag ctctgatttt ttcctttccc catgccgggt tttcgcccgg ctttttcagg
    12961 agtcattaat tatggcaaag actatccttg ccccgtcact gagtgaacgg gtctatacgg
    13021 gtacgcacgg taatgagtcg gtggcagaag gcgtatttac ggtgaatgct gcggaagcgg
    13081 acagtgttat tcatcttctc tcactgccag tgggcatccg tatcaactca ctccagctgg
    13141 tttcaacggg tggtctgggt actgcaaccg tcagcattaa gtccggtgag catgttctca
    13201 tcgataacag cgaagctgtt tctgcaaaat ttgccagata tgtgccagtg gagccgtaca
    13261 ccacacagcg tgacggggag ctggttactg tcaccattaa gactgccgct gcaaccggca
    13321 ccctgaatgt tctgctgcgt tataccgtgg tgggatactg attaaaacct tccggcccgc
    13381 gtcatgcggg ctttttatcc ggggaattat atgagtgaga aaattgccgt tgtctatatc
    13441 ggcccaaaaa ccgtgaaaaa ggacaccatt accggaagcc gcacattgtt cccacgtctt
    13501 gagccggtgc atgttgacag tgcgatggcc tggcaactgc tggggtttcc ggatgtctgg
    13561 gttcgtcatg aagagcttga tgatgttctg aaaaagcaac aacagaatga gcagttgcgg
    13621 caggcacagc aggcgcagga aagagtgctt gctgcgcggg cagaagcgga gaacagtttt
    13681 gttgtttctg ttaacgggca ggaggtggat ttaagtaagc tcacctcagc acggctggcg
    13741 acgctgtgtg aggcagaaga gctggatatt cacaaagacc cgaaagaaac ggctgaggca
    13801 ttccgtatcc gggtgcgtga ggcatttcgc cgtcgtgttg cggagactga acagcatggc
    13861 ggaactgagt gattttttac cgtatgtccg tcgtcatatc agcggtccac tgaacattat
    13921 gatgacggat gctctgtcaa tggctgccgt ggcattcagc cgccagtcgt tggtgtgccg
    13981 tcgggaggtt actgttgtac cagtagcagg aaaagaaatc gtgcttccgt atgacaaaga
    14041 tgatgaggag tgcgttcata tcatccgtat ctctgacgat aatcatgagc tttttgtcgg
    14101 tcgggatgtg gatatcagct ccggacgctc cctgcgattt gcctgttctc ccggtgaggt
    14161 gagcgtgctt tatgccgtcg ctccgaaagc cggacgcagc cagataccgg atgaactcct
    14221 cacatggcct gaagaagtgg ctgcgggggc acttgagcgg ttgttcatgc agactggtgt
    14281 ttcatggtca gatccgttac gcgcacagta tttttctgtg cagttttctg aggggatccg
    14341 tcgggcatat cgtcatacac tggcgacaag cccgtactct tcataccgca accctgtacg
    14401 caggcagagg ttttactgat gacgacgatt acagaaatca tcggacgtgt gaatacacaa
    14461 ctggttgacc cgatgatggt tcgctggccc ctgcaggaat tgtgcgatta ttacaatgat
    14521 gctgtgaggg cagtgattct ggcgagaccg gatgctggcg caagcctgga aacaatcagt
    14581 tgtgttcctg gcgcccgtca ggttttgccc gatggtgtaa tacaacttct tgacgtgata
    14641 tgcctcagtg acggtagtgc agtcagacca ttatcccggg aggtgctgga tgcgcagtat
    14701 cctgagtggc ccacaatgaa gggcattcct gaatgtttta tcagcaacga cctgtccccg
    14761 cgcgtattct ggctgtttcc tgtctctgac aaagagataa gtattgatgc agtggtaagc
    14821 cggataccgg aggcagtgta tgttctgacg caggacgatg atacgccagt tccactggaa
    14881 gaggcttatg ttaacccact ggtggactgg atgttgtttc gtgctttcag taaggatgct
    14941 gccggtggtg cagaatcggg gctggctgcg cagcattatc agagttttgt tgagcaactt
    15001 gggatcaaac agggggcaga cagtgcattg tctgcccgta aaaaagtgtt taacggaggt
    15061 ggagtgtgag tgttgttgtt tcggggacgc tgaaatctcc tgatggtgag gcgatatcag
    15121 gagcaaatat taccctgacg gcgctgacag tttcaccgga tgcgctcagc ggcaccagtg
    15181 cgtcggcagt gacccgtgaa ggtggatatt acggaatgac gatggatccg ggggagtatg
    15241 cggtttcggt gacggtgaaa gggaagacag ctgtctacgg acgtgtgcgt attgagggga
    15301 ccgaaagtac ggtgacgctc aatatgctgt tacgccgcag tcttgttgag gtgagcatac
    15361 ccggagagct gctgacagat ttccggcaga tacagaataa tgtggctgat gaccttgcca
    15421 ctattcgtcg cctgaatgaa gacacggcga caaaaaacac tcaggccaca cagtcaaaag
    15481 aaagtgcagc agccagtgcg aagagtgcat ctgacagtgc aaagacggca accagcaggg
    15541 cggctgaagc cggacaaaaa gccagtgaag ctgcagaagc agcgacccgg gcaggcgagt
    15601 ctgaaaaggc agcggcagca gacgcgaaaa atgcaaaaca acatgctgaa acagcccggg
    15661 tggctcagca ggcagccgga gacattcttg agcgtgcgga ggctgcaacg gtcagtgcag
    15721 aggaagcccg gcgcatggct gagaatgcgc ggggacctca gggcgaccag gggcccaaag
    15781 gtgataccgg cccccggggt gaaaaaggtg agccgggtca gagaggtcct cagggtccga
    15841 aaggagagcg cggtgagaga ggtcctcagg gtagtgtcgg cccggcaggt ccgcggggtg
    15901 agaaaggcga gcagggggag cgtggaccac agggattaca ggggctgaaa ggtgaaaccg
    15961 gagaacgtgg agcacagggc gcacagggac cggcaggccc gacgggggca acggggcctg
    16021 ctggtcctcg tggtgagacg gggccacagg gtgcacaggg gccagccgga gcaacaggac
    16081 ctgttgggcc acaaggagag cgcggtattc aggggcctgc cggtcctgcg ggcccgcgag
    16141 gtgaaaccgg agccagaggt gaaaaaggag accccgggga ccccggcggc ccaccgggac
    16201 caaagggtga taccggacct gctggccctg ccggtgaaaa aggtgaaccc ggtgagcgag
    16261 ggccacaggg attacaggga ccaccgggtc ctgccggaga gagaggtccg gcaggcgaac
    16321 cgggagcgaa aggtgaaacc ggaaaacccg gacctgctgg tcctcagggg cagcccggtc
    16381 cacagggact taaaggtgac aggggcgagc caggacctac aggcccacag ggaccggcgg
    16441 gagagcgggg acctcagggg cctcgggggg tagctggtcc tgtcggcccg gcaggtgcag
    16501 cgggcgctaa aggtgatgca ggacctgcag ggcctgccgg tccccgagga cccgccggtg
    16561 aacggggccc tgccgggccg aaaggtgagc gtggtgaacc gggaccgatg gggcctccgg
    16621 ggccagcagg cgacgcagga aacagcacag taaaaggcat tcgtctggga aatgagatgg
    16681 tgtaccgtcc atatagtggc tatagcaact tgagcctacg ctcaatggat ggctgtgtgc
    16741 ttacgggaat tgcgggaaca aacatgccag acaagagccg tatagatgaa atctactaca
    16801 gacccttgca aatgactttt ggtgatggaa gctggcgctc agtcggtata ggggaagtgt
    16861 gagctgttgt tcttttcagc gcaggtaact ggcagctctg catacttgtt atattgcgca
    16921 taaaaaaccg gggagcaggg cgttactccc cggtaaacat acagaccaga caatgatacc
    16981 acactgtcag ttattattat catgacataa gtcagcgcag ggaatattag tgcatgcaca
    17041 tgtctggttt ctttgatctc acaacgcttt ttttgtgaaa agaaaaaaga tgaggcactg
    17101 aaatcacagt tttatttaaa tgagtggacg ttgatgatta ttaagagttt taagctatac
    17161 acccctgaaa aatctgacgt gccgggtgcg atgtacctga aatcagtaga cggtagggac
    17221 tggtacgaaa gccagtcatt attcgcggca gacacgctga agctggttta tgacagtaac
    17281 ggtataatca ccagcataag taaggatgtc tctatgctgt ggccggtaaa ccagagtgtg
    17341 gcagaggttg ctgatacgga agaaaaccgc aaagctgaca tctcggggcg ctggggtttt
    17401 gacggggaga aaatcacgga cctgctgacc gcggagaaag cgcgcgggat gaagggcgat
    17461 gaaattaacg cctggcgtaa tgagatggaa gcggcgaact acacgtttga gcacaatggg
    17521 cgtaaatggg actacgggaa gtcaacgcag acgcgtcttg agccgtcggt ggcggcagcg
    17581 aaagcgggga aactgccgga ggcgtttttc tggacggatg cggaaaacaa tgatgtgccg
    17641 gtgacagcag aagagcttat agcactgagt gaagcggcag agcaggcgat gttcaccaaa
    17701 gggatggaaa tccacgtccg gcagcgcacc atgaagaagg agctggaaaa gctgaccagt
    17761 gcggatgaga tactggcata cagggtggac tggaaataaa aagaagggga gcggatgcac
    17821 ccctgatatc t
//
LOCUS       DAZGSP010000103        17815 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZGSP010000103 DAZGSP010000000
VERSION     DAZGSP010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.104"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..160
                     /locus_tag="ACHK74_003916"
     CDS             <1..160
                     /locus_tag="ACHK74_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222651.1"
                     /translation="FVAFRAFREKKKLRLHVTSAYPISEKQKGKSVKFFTIAYNLLRN
                     KQLPQPSK"
     gene            complement(570..1703)
                     /gene="thiH"
                     /locus_tag="ACHK74_003917"
     CDS             complement(570..1703)
                     /gene="thiH"
                     /locus_tag="ACHK74_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFP0222652.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(1700..2470)
                     /gene="thiG"
                     /locus_tag="ACHK74_003918"
     CDS             complement(1700..2470)
                     /gene="thiG"
                     /locus_tag="ACHK74_003918"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFP0222653.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(2472..2672)
                     /gene="thiS"
                     /locus_tag="ACHK74_003919"
     CDS             complement(2472..2672)
                     /gene="thiS"
                     /locus_tag="ACHK74_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFP0222654.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(2656..3411)
                     /gene="thiF"
                     /locus_tag="ACHK74_003920"
     CDS             complement(2656..3411)
                     /gene="thiF"
                     /locus_tag="ACHK74_003920"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFP0222655.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(3404..4039)
                     /gene="thiE"
                     /locus_tag="ACHK74_003921"
     CDS             complement(3404..4039)
                     /gene="thiE"
                     /locus_tag="ACHK74_003921"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFP0222656.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(4039..5934)
                     /gene="thiC"
                     /locus_tag="ACHK74_003922"
     CDS             complement(4039..5934)
                     /gene="thiC"
                     /locus_tag="ACHK74_003922"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFP0222657.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPITAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(6015..6115)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(6167..6643)
                     /gene="rsd"
                     /locus_tag="ACHK74_003923"
     CDS             complement(6167..6643)
                     /gene="rsd"
                     /locus_tag="ACHK74_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFP0222658.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            6738..7511
                     /gene="nudC"
                     /locus_tag="ACHK74_003924"
     CDS             6738..7511
                     /gene="nudC"
                     /locus_tag="ACHK74_003924"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFP0222659.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            7551..8615
                     /gene="hemE"
                     /locus_tag="ACHK74_003925"
     CDS             7551..8615
                     /gene="hemE"
                     /locus_tag="ACHK74_003925"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFP0222660.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLVRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            8625..9296
                     /gene="nfi"
                     /locus_tag="ACHK74_003926"
     CDS             8625..9296
                     /gene="nfi"
                     /locus_tag="ACHK74_003926"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFP0222661.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            9339..9929
                     /gene="yjaG"
                     /locus_tag="ACHK74_003927"
     CDS             9339..9929
                     /gene="yjaG"
                     /locus_tag="ACHK74_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFP0222662.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            10116..10388
                     /gene="hupA"
                     /locus_tag="ACHK74_003928"
     CDS             10116..10388
                     /gene="hupA"
                     /locus_tag="ACHK74_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFP0222663.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            10401..11096
                     /gene="yjaH"
                     /locus_tag="ACHK74_003929"
     CDS             10401..11096
                     /gene="yjaH"
                     /locus_tag="ACHK74_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFP0222664.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(11098..11523)
                     /gene="zraP"
                     /locus_tag="ACHK74_003930"
     CDS             complement(11098..11523)
                     /gene="zraP"
                     /locus_tag="ACHK74_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFP0222665.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            11761..13137
                     /gene="zraS"
                     /locus_tag="ACHK74_003931"
     CDS             11761..13137
                     /gene="zraS"
                     /locus_tag="ACHK74_003931"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFP0222666.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKCSTFTLWLPVN
                     ITRKDPQG"
     gene            13134..14459
                     /gene="zraR"
                     /locus_tag="ACHK74_003932"
     CDS             13134..14459
                     /gene="zraR"
                     /locus_tag="ACHK74_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFP0222667.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNIALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERSVVLLTGEYISERELPLAIAGTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(14456..15745)
                     /gene="purD"
                     /locus_tag="ACHK74_003933"
     CDS             complement(14456..15745)
                     /gene="purD"
                     /locus_tag="ACHK74_003933"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFP0222668.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNIYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(15757..17346)
                     /gene="purH"
                     /locus_tag="ACHK74_003934"
     CDS             complement(15757..17346)
                     /gene="purH"
                     /locus_tag="ACHK74_003934"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFP0222669.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         4008 a         4787 c         4903 g         4117 t
ORIGIN      
        1 ctttgttgcg ttcagggctt tcagggaaaa gaaaaaactc cgtttgcatg taactagcgc
       61 ttatcccatt tctgaaaaac agaaaggtaa atcagtgaaa tttttcacca ttgcctacaa
      121 cttattgaga aataagcagc ttcctcagcc ctcaaaataa caaaacccac cttaaggtgg
      181 gtttcgccag agaattatct ctggtattca gaacgccatt accggacttt gccttgacct
      241 tgcgataatc gcaggttgcg ggatgtctga atttcttcag tctgctgcat cctggaagat
      301 gagaacatgt gttcttattt tcgtctctat catagttgag tatttactct cttacaatca
      361 gatctctttc attgctcaac aggcgatggc ttcagacttt gcattacgga atttttaaga
      421 aaggcagggc gaaacgagga agaagctttt cgccctggac aacattcctg ctgacggcac
      481 taccataaaa aaacagaacg ctttcaacaa ccttgcgttt ttcatcttcc gtagcggctc
      541 ggaataaatt tacgttctta cattccgtct catagtcttt gcgaggcccg tcccagatag
      601 ctgtcccagt ctttccatac cggctgcaaa ccctgagcgg ttaacgcggc agcaaccgct
      661 tccggtctgc gatcgtcgtg cggtgagaac tgttccaact cggggtgatt atcggcatag
      721 ccacctggct gcgttttcga gaaggcgctg acgttattga tcgccagcgg aataacgcga
      781 tcgcgaaacc acggtgattc ccgcgtggag agtgacagtt caatctccgg tgcaagcagt
      841 cggaaggcgc agatggtttg cactaactgg cgttcatcca taatcgacgc aggctcaatg
      901 ccgccagtac acgggcgcag gcgcggaaag gagacagagt aacggctttg ccagtaatgc
      961 tgttgcagcc atagcaaatg ttcggcaacc atatagcagt caacgcgcca gttgtcggaa
     1021 aggccaatta gcgcgccgag gcctatctta tcaatccccg cacgccccag ccgatccggc
     1081 gtttccagcc gccagaagaa gtcctgtttt tttcctttca gatgatggcg ggcataagtc
     1141 gcctcgtgat atgtctcctg ataaaccatc acgccatcca gaccaagctg ctttaactcg
     1201 gcgtattccg tctccgccag cggttgcact tccatttgta gtgaagagaa ctgttcacgc
     1261 agggcgggga gatgacgacg aaagtaatcc atccccactt tcgcctgatg ttcaccagtg
     1321 actaatagca gatgttcaaa gcccatctcc cgtatagcgg cgctttccct ggcaatatcc
     1381 gcttcatcca gcgttttgcg cttgatgcga ttactcatgg aaaatccgca gtacgtgcag
     1441 tcgttagcgc aaagattgga aagataaagc gggacgtaga aactaactgt gttgccaaat
     1501 cgctgacggg tcagacgctg cgcccgttgg gccagttgtt ccagatagcc actggcggct
     1561 ggcgataaca gcgccatcat atcgtcgcgg gtgagttgcg aggcatttag cgcccgttct
     1621 acgtcagcag ccgttttgcc gttgatacgc aggcggatgt cgtcccagtc cagttgtcgc
     1681 cagcgatcgc tgaaggtttt catgccgatg cctccagaaa tccagtcagc gggctggtgg
     1741 catgagcaaa atgactgcgg ctgcccggtc cggactgacg tgccagcagg cctgcttcta
     1801 ccgccagacg aaatgccttc gccatgttga cgggatcgtc cgcgacggca attgccgtat
     1861 tcactaacac cgcgtcggcc cccatttcca gcgcctgcgc ggcatggctg ggaacgccga
     1921 tgccagcatc gacaaccacc ggcactgtgg cctgctggat aataatctcc agcatggcgc
     1981 gggtttccag tccctgattc gagccaatcg gcgcgccgag cggcatcacc gctgcacagc
     2041 cgacttcttc cagacgttta cacaataccg gatcggcccc gcagtaaggc agcacgacaa
     2101 atccctgttg taccagcgtt tcggcggctt tcagggtttc gatgggatcg ggcaacagcc
     2161 agcgggcgtc agggtgaatc tctaatttta accagtttgt gcctaacgct tcacgagcca
     2221 gatgggcggc gaaaatggct tcttccgctg ttttcgcccc ggatgtattt ggcagcaggg
     2281 tcacacccgc cgcgataagc ggttcgagga tagcgtcgtt gtgctggcgc aagtcgacac
     2341 gtttcatcgc cagtgtcacc agctggctgc cggaagcgcg gatcgcctcc accatcagtt
     2401 gtgaagaagc gaatttccct gtgccggtaa acagatgtga atcaaacgtt ttgtccgcaa
     2461 tacgtaacat ttcaaccccc tgcaataacc tgaaaaagca ggatctggtc gccatcctgc
     2521 acgatatgtt gcgcccactg ctcacgcggg acgatttgct gattaatcgc cagagccgcg
     2581 cccgcttgtc gttggtcgag ttgctccagt agttcgtgaa cagtttgccc ggcggcgcac
     2641 tgcatcggtt gatcgttaaa caggatctgc attgcttcct ccgcataccg ggcaaccact
     2701 ggcgcggcgc aacgccaggc tgcgccactg gctcgattta ccgtcgaaca gtcggagttc
     2761 tcccgcaggt gtctctatac cgcttaataa cttaatggct tccagtgcct gcaaagtgcc
     2821 cataaccccg accaccgggc caaccacgcc cgccgtgcgg cagttgcgtt ctggctcctg
     2881 gttatctggc cacaggcagc ggtaacaccc ctgctcccag ggcggcgtca gtaccatcaa
     2941 ctgaccgcca aatccgaccg cgctggcggt gataagcggc gtgttgagtg ccacgcaggc
     3001 ggcattaatc tcctggcgag tcgccatatt gtcggtacag tcgagcacca catcggcccg
     3061 tgcaaccgca tcttttaacg cctcacccgt taaccgttgt tgtaatgctg tcagttgaat
     3121 gtcgggattc aactgtgtca gtcgctgttg gctgacctgc gatttcgggc tgttgatatc
     3181 ttcagtggta aagaggattt gtcgttgcag attgcttaaa tgcacatcgt cgtcatctgc
     3241 cagtaccagc gtcccgacgc cagcgcccgc caggtacagc gcagcaggtg tacccagccc
     3301 gcccagaccg ataatcagca cctggctgtc gagcagtttt tgctgcccgt ccagagcgat
     3361 atcgtcgagc aggatttggc ggctataacg cataaagtca cggtcattca tcgccaactc
     3421 ctgcaatttc cagcaactgt gccgttgcca aacgccagtc tgcagcttga gtaatggcgc
     3481 tgacgacggc gatactgccg acacccgttg ctatcaccgc aggcgcgcgt gccagactga
     3541 taccgccaat cgccacggtg ggataatccg ccagtcgctc aacatgccgt gccagctgtt
     3601 ccagcccctg cggtgcagaa ggcatctgtt tggtttgcgt cgggaacaca tgtcccagcg
     3661 cgatataaga ggggcgtgct gccagcgcga cgtcgatttc catatcgtca tgtgtcgaaa
     3721 cgcccagccg caggcctgcc gcgcggatgg cattgagatc ggtggcttgc aaatcttcct
     3781 gccccaaatg gacgccatac gcctgatgct tgatcgccag ccgccagtaa tcgttgataa
     3841 acaatcgcgc gttatagcgg cggcccagcg caattgccgc cacgacatcg gcttccacct
     3901 cttcatcgcg ccgatctttg atgcgtagct ggagagtacg tacgcctgca tccaacagac
     3961 gttcgatcca ctgtacgctg tccaccaccg ggtacagtcc taaacgaaaa ggtacaggag
     4021 gaaaatcagg ctgatacatc acgcttcctc cttacgcagg tagatttctc cgcctctggc
     4081 acggaagttc tccgacatat ccgccattcc catttcaata gtttgcgtgg cggcgtaatc
     4141 acgcacttcc tggctgattt tcatcgagca gaatttcggc ccacacatgg agcaaaaatg
     4201 ggcgacttta cctgactctt gcggcagggt ttcatcgtga taagcgcggg cggtaaacgg
     4261 gtcgagggcc agattaaact ggtcttccca gcgaaattcg aagcgggctt tcgacatggc
     4321 gttatcgcga atttgcgcgc ccggatgccc tttcgccagg tcagcggcgt gggcggcaat
     4381 cttataggtg ataagcccct gcttaacatc ttctttatta ggcagaccca gatgctcttt
     4441 tggcgttacg taacagagca tcgcgcagcc aaaccagcca atcatcgccg caccaatccc
     4501 cgacgtgaag tggtcatagc ccggcgcaat atcggtagtt agcggcccca gagtgtaaaa
     4561 cggcgcttcg tggcagtgct ctaactcctc ggtcatattg cggcggatca tctgcatcgg
     4621 cacgtggcct gggccttcaa tcatcacctg cacgtcatat tcccaggcaa ttttggtcag
     4681 ttcgcccagc gtatgcagct cggcaaactg cgcttcatcg ttggcgtcct gaatagaacc
     4741 ggggcgcaga ccgtcgccca gcgacagcga aacgtcataa gcggcacaga tttcacaaat
     4801 ttcgcggaag tgttgataga ggaaattttc ctgatgatgg gagaggcacc atttcgccat
     4861 aatcgaaccg ccgcgagaga cgataccggt caggcgtttc gcggttatcg gcacatagcg
     4921 cagcagtacg cccgcatgga tagtgaagta atccacacct tgctcggcct gttccagcag
     4981 cgtgtcgcgg aacgcttccc aggtaagatc ttcggcgatc ccgttaacct tctccagcgc
     5041 ctggtagatc ggcactgtac cgatcggcac cgggctgtta cgcaaaatcc actcgcgggt
     5101 ttcgtgaata tagcgaccgg tggagagatc catcaccgta tccgctcccc agcgcgtgga
     5161 ccataccagc ttttccactt cttcttcgat ggaagaggtg accgccgagt tgccgatatt
     5221 ggcgttaact tttaccagga aattgcgacc aataatcatc ggctccgatt ccggatgatt
     5281 aatgttggcc gggataatcg cacgtccggc agcaacttca tcacggacaa attccgcagt
     5341 gatattttcc ggcagatgtg cgccaaagct cattcccgga tgctggtggc gtaaaacctc
     5401 gctacggatg cgctcgcggc ccatattctc gcggatggcg atgaattcca tttccggcgt
     5461 gatgatgccc tggcgggcgt agtgcagttg ggtgacacgg cgtcctgctt tggcgcgttt
     5521 tggtgttagt acgccgctaa aacgcagttc gtcgaggcca tcatctgcca gccgcgcttt
     5581 agtgtaatcg gaactgcgca cggtaagttc ttcggtatcg ccgcgcgcat cgatccacgg
     5641 ctggcgtagt tttgccagcc cttgctgcac gttaatggca atctgcggat caccatacgg
     5701 gccggaggtg tcgtagaccg gaatcgcttc gttttcttcg tactgcggct gttctttgct
     5761 accgccaatt agcgtcgggc taagctggat ctcacgcatc ggcacgcgca cgccggggtg
     5821 tgtgccagtg atataaatgc gttttgagtt gggaaaggcg gtgccttcca gggtgtcgat
     5881 aaaatgttgg gcccgggcgc gttgttcgcg gcgggtcagt tttgttgcag acatagctca
     5941 ttccaaaaag ttaaggacgt ggcttgtcag acgacggatg aagcaagaga cgatcgccgc
     6001 aggggcgatg cgatagcaga ttaactcttg ttcccttcgc aggtattagc ctgatcaggt
     6061 tccgcggatc ccgaataaac ggtctcagcc agttaaggca ctccgacaag aaattagccc
     6121 cgcaaatggg gcattgaatg taaattacgc gttaacagcg cagaactcaa gcaggatgtt
     6181 tgacgcgggc ggcgtcaagc accagcagaa tcagcttatc ttccagcaca aagcgcgctt
     6241 ccagcgcctc gccgatgtca gataaaacct gttgaaactc aaggtaatta tcatgatcga
     6301 tggcggtttc cagactggaa tcgtagtaat ccataatctg ttgagtattg gcttcgagtt
     6361 gcggccaaat cttcgcggcg cgcgcgagtt gcccgttgcc ttccagctta tgaagaatgc
     6421 gctcataaat actgaaatgt ccggcagaca agtaatcgac caggctctga caaaaatcat
     6481 caagggcttt ttcgtttagc ctcatgtacg attctttgcc aggcttaatg ccaaccagat
     6541 tgtagtaagc cacgagcaga tgcttacgta catgtagcca gcgatcaacc agtttgttac
     6601 ttcctctgac gcgctccgtc aggttatcga gctggttaag catgattgac tccgcaagtt
     6661 tgtattcaaa aactgctcag tgagaaatgt aaaaaccatg ttaaacatgc cagtgatgca
     6721 aaggtagtgc aagagctatg gatcgtataa ttgaaaaatt agatcacggc tggtgggtcg
     6781 tcagccatga acaaaaatta tggttgccga agggagaatt gccatatggc gaagcggcaa
     6841 atttcgatct tgtgggtcag cgcgcactac aaatcggcga atggcagggg gaacctgttt
     6901 ggttagtaca acagcagcgg cgtcacgata tggggtcggt acgtcaggtc attgatctcg
     6961 atgttgggct gtttcaactg gccggacgag gcgtacaact ggcggagttt taccgatcgc
     7021 ataaatactg tggttactgc gggcatgaaa tgtatccgag caaaaccgaa tgggcgatgc
     7081 tgtgcagcca ttgccgtgag cgttactacc cgcaaatcgc cccctgcatt attgttgcca
     7141 tccgtcgcga tgattcgatc ctcctcgccc agcatacccg ccatcgtaac ggtgtccata
     7201 cagtacttgc cggattcgtc gaagtgggcg aaaccctcga gcaggcagtc gcgcgggaag
     7261 tgatggaaga gagcggaatt aaagttaaaa acttgcgtta cgtgacttct cagccgtggc
     7321 cgtttcctca gtctttaatg accgcgttta tggcggaata tgacagcggc gacatcgtga
     7381 tcgacccgaa agaattgctc gaggcgaact ggtatcgcta tgacgatttg ccgttactcc
     7441 cgccgcccgg caccgtagcg cgccgtctga tagaagatac ggtggcgatg tgtcgggcag
     7501 agtatgagtg atgatacact gaccgcctga cgcactaagg aacagccaaa atgaccgaac
     7561 ttaaaaacga tcgttatctg cgggcgctgc tgcgccagcc cgttgatgtc actccagtat
     7621 ggatgatgcg ccaggcgggt cgctatctac cggaatataa agccacgcgc gcccaggcgg
     7681 gcgattttat gtcgctgtgc aaaaacgccg agctggcgtg cgaagtgact ttgcaaccgc
     7741 tgcgtcgcta cccgctggat gcggcgatcc tcttttccga tatcctcacc gtgccggacg
     7801 cgatggggtt agggctctat tttgaagccg gagaaggtcc gcgttttacc tcgccagtca
     7861 cctgcaaagc cgacgtcgat aaactgccaa ttccggaccc ggaagatgag ctgggttacg
     7921 tgatgaacgc ggtgcgtacc attcgtcgcg aactgaaagg cgaagtgccg ctgattggtt
     7981 tttccggcag cccgtggacg ctggcgacct acatggtgga aggcggcagc agcaaagcct
     8041 tcaccgtgat caaaaaaatg atgtatgccg atccgcaggc gctgcacgct ctgctcgata
     8101 aactggcgaa aagcgtcact ttgtatctga atgcgcagat taaagccggt gctcaggcag
     8161 tgatgatttt cgacacctgg ggcggcgtgc ttaccgggcg cgattatcaa cagttctcgc
     8221 tctattacat gcataaaatt gttgatggtt tagtgcgtga aaacgacggt cgccgcgtac
     8281 cggtcacgct gtttaccaaa ggcggcggac agtggctgga agccatggca gaaaccggtt
     8341 gcgatgcgct gggcctcgac tggacaacgg acatcgccga tgcgcgccgc cgtgtgggca
     8401 ataaagtcgc gttgcagggt aatatggatc cgtcgatgct gtacgcgccg cctgcccgca
     8461 ttgaagaaga agtagcgact atacttgcag gtttcggtca cggcgaaggt catgtcttta
     8521 accttggtca cggcattcat caggatgtgc cgccagaaca tgctggcgtg ttcgtggagg
     8581 cagtgcatcg actgtctgaa cagtatcacc gctaaggagt gattatggat ctcgcgtcat
     8641 tacgcgctca acaaatcgaa ctggcttctt ctgtgatccg cgaggatcga ctcgataaag
     8701 atccaccgga tctgatcgcc ggagccgatg tcgggtttga gcagggcgga gaagtgacgc
     8761 gagcggcgat ggtgctgctg aaatatccct cgcttgagct ggtggagtat aaagttgccc
     8821 gcatcgccac caccatgcct tacattccag gttttctttc cttccgcgaa tatcctgcgc
     8881 tgctggcagc gtgggagatg ctgtcgcaaa agccggattt agtgtttgtc gatggtcatg
     8941 ggatctcgca tcctcgccgt cttggcgtcg ccagccattt tggcttattg gtggatgtgc
     9001 cgaccattgg cgtggcgaaa aaacgtctct gcggtaaatt cgaaccgctc tccagcgaac
     9061 cgggcgcgct ggccccgctg atggataaag gcgagcagct ggcctgggtc tggcgcagca
     9121 aagcgcgctg taacccgttg tttatcgcta ccggccatcg ggtcagcgtg gacagcgcgc
     9181 tggcgtgggt acaacgctgc atgaaaggct atcgtctgcc ggagccaacg cgctgggcgg
     9241 acgcggtggc ctcggaacgt ccggcgttcg tgcgctatac agcaaatcag ccctaattca
     9301 ggtaaactgc ggataatttc cgtatttgag aactcaacat gttacaaaac ccaattcatc
     9361 tgcgtctgga gcgcctggaa agctggcagc atgtcacttt catggcttgc ttatgcgaac
     9421 gcatgtaccc caattacgcc atgttctgcc agcaaaccgg ttttggtgat gggcaaattt
     9481 accgtcgtat tctcgatctc atctgggaaa cgctgaccgt taaagacgca aaagtaaatt
     9541 tcgacagcca actggagaaa tttgaagaag cgattccttc agccgacgat ttcgatctgt
     9601 acggcgttta tccggcaatc gatgcctgcg tggcgttaag tgaactggtc cattcgcgtt
     9661 tgagtggtga aacgctcgaa cacgcggtgg aagtgagtaa gacctccatc acgaccgttg
     9721 cgatgctgga aatgactcag gctggtcgcg aaatgagcga tgaagagctt aaagaaaacc
     9781 cagctgtaga gcaagaatgg gacattcagt gggaaatatt ccgactttta gctgagtgcg
     9841 aagaacgcga tatcgagctg ataaaaggcc ttagggcaga cctgcgtgag gcgggtgaga
     9901 gcaatattgg tataattttt cagcaataag accagaaaac gtgatttaac gcctgatttg
     9961 tcgtacctgg agtcttccct ttcgcccccc gtctggtcta catttggggg gcgaaaaaaa
    10021 gtggctatcg gtgcgtgtat gcaggagagt gctattctgg catttccgtc gcactcgatg
    10081 cttagcaagc gataaacaca ttgtaaggat aacttatgaa caagactcaa ctgattgatg
    10141 taattgcaga gaaagcagaa ctgtccaaaa cccaggctaa agctgctctg gagtccactc
    10201 tggctgcaat tactgagtct ctgaaagaag gcgatgctgt acaactggtt ggtttcggta
    10261 ccttcaaagt gaaccaccgc gctgagcgta ctggccgcaa cccgcagacc ggtaaagaaa
    10321 tcaaaattgc cgcagctaac gtaccggcat ttgtttctgg caaggcactg aaagacgcag
    10381 ttaagtaaga ttgcgtggca gtgaacagtt ttaacgaagg ggtggtttca ccccttttgt
    10441 ctttctggcg tcgatcattg atgctggctg gcgctctgct tctcactgcc tgtagtcata
    10501 actcttcact tcctcccttt accgccagtg gatttgctga agaccagggc gcggtacgca
    10561 tctggcgaaa agacagcggc gataatgtgc atctgcttgc cgtgtttagc ccgtggcgca
    10621 gtggcgatac cacgacgcga gagtatcgct ggcagggcga taacctcacg ctcatcaata
    10681 tcaatgttta cagcaaaccg ccggtgaata ttcgcgcgcg ttttgacgat cgcggtgatc
    10741 tgagctttat gcaacgtgaa tccgatgggg aaaagcagca gctttctaac gaccaaatcg
    10801 atttataccg ttatcgtgct gatcagatcc gccagattag cgatgcctta cgtcagggga
    10861 gagtcgtgct gcgccagggg cgctggcatg cgatggaaca gaccgtgacc acctgcgaag
    10921 ggcaaaccat taaacctgat ttagattcgc aggcgatagc gcatatcgag cgccgccaga
    10981 gccgctcttc tgttgatgtc agcgtggcat ggctggaagc gcccgaaggt tcgcaattac
    11041 tgttagtggc aaactctgat ttctgtcgct ggcaacccaa cgagaaaacg ttctgattta
    11101 ccagtggccc atacccatat gaccgccacc accgcagccg ccgtagccca tgcccattcc
    11161 ggctccgcgc ggaatacccg cttcagccat cgcgatatct cgtttcaccc gtaactcatc
    11221 taacgactga cgcaaattct ccatctcttt ggcgaccgcg ttaattttgc tgctatccgg
    11281 tgggttcgcg gctaacaggg cattgtattc ataacgcttc gtcaccagtt gctgttgcag
    11341 tgcgctgctt tgagcgtaaa agtcattatg gattttctgc cacgctgtct gttgttcgct
    11401 ggtcaaaggc gcggcatttt gctgccacat accgtgtccg ccgtgagcaa atgcagatgt
    11461 cgatcccatc gccattgctg aaagcgccat cattaccagg gcaattttcg tgttccgttt
    11521 catggttaat cctccagtgg ttgtcttact tcgggtattg catcttccgt gccaacgatg
    11581 aaacgctgat atgacgggta atctggcatg ataaatgagt aaaaatgact cgcctgctgc
    11641 gggtagcgag ttatttttac tcattgaaac ttaagccttt gtgttacagc gcagggtaag
    11701 cgctgataaa agatggcatg atttctgctg tcagaaaggg atgagcaggc aaagaagaag
    11761 atgcgtttta tgcaacgttc taaagactcc ttagctaaat ggttaagcgc gatcctcccc
    11821 gtggtcattg ttgggctggt ggggttgttt gcggtgacgg tgattcgcga ttatgggcgc
    11881 gagactgccg ccgccagaca aacgctgctg gaaaaaggca gtgtacttat tcgcgctctt
    11941 gaatccggct cgcgcgtcgg catggggatg cgcatgcatc atgcgcagca gcaggcctta
    12001 ctggaagaaa tggccgggca gcctggagta cgttggtttg cggtcacgga tgaacaagga
    12061 acaatcgtga tgcatagcaa ctccggcatg gtgggaaaac agctttattc cccgcaggaa
    12121 atgcagcagt tacatccggg agatgaagaa gcgtggcggc ggatcgatag cgcagacggc
    12181 gagcctgttc tggaaattta tcgccagttt caaccgatgt ttgctgctgg aatgcaccgg
    12241 atgcgccata tgcagcagta tgccgcgaca ccacaagcaa ttttcatcgc tttcgacgcc
    12301 agtaatattg tgagtgccga agatcgtgag cagagaaaca ccctgattat tctcttcgcc
    12361 ctggcgacgg tcttgctggc aagcgtattg tcattcttct ggtatcgccg ctatctgcgc
    12421 tcgcgccagc ttctacaaga tgaaatgaag cgcaaagaga agctggtggc gctggggcat
    12481 cttgcggcag gcgttgccca cgaaatccgt aacccacttt cctcgattaa aggactggcg
    12541 aaatactttg ccgagcgcgc gcctgcaggg ggagaagcgc atcaactggc gcaggtgatg
    12601 gcgaaagagg ccgaccgttt aaaccgcgtg gtaagcgagt tgctggaact ggttaagcca
    12661 acgcatctgg ctttgcaggc ggtggatctc aacacgctga ttaaccactc attacagctg
    12721 gtaagccagg atgcaaacag ccgggagatc cagttacgct ttaccgccaa cgacacatta
    12781 ccggaaattc aggccgatcc ggacaggctg actcaggtcc tgttgaatct ctatctcaat
    12841 gctattcagg cgattggtca gcatggcgtg attagcgtga cggtcagcga aagcggcgcg
    12901 ggcgtgaaaa tcagcgttac cgacagcggt aagggaattg cggcagatca gcttgaagcc
    12961 atcttcactc cgtacttcac caccaaagcc gaaggcaccg gattggggct ggcggtcgtg
    13021 cataatattg ttgaacaaca cggtggtaca attcaggtcg caagccagga gggaaaatgc
    13081 tcaacgttca ccctctggct tccggtcaat attacgcgta aggacccaca aggatgacgc
    13141 acgataatat cgatattctg gtggtggatg atgacattag ccactgcact attttgcagg
    13201 ctttactgcg cggctggggc tataacatcg cgctggcgaa cagcgggcga caggcgctgg
    13261 agcaggtgcg ggaacaggtt tttgatcttg tgctttgcga tgtgcgaatg gcggagatgg
    13321 acggcatcgc cacgctgaaa gagatcaaag cgttaaaccc ggcaattccg gtgctgatta
    13381 tgactgcgta ctccagcgtc gagacggcgg tagaggcact gaaaactggg gcgctggatt
    13441 atctcatcaa gccgctggat ttcgataacc tacaggcgac gctggaaaag gcgctcgcgc
    13501 atacgcacag tattgatgct gaaacacctg cggtgactgc cagccagttc ggtatggtcg
    13561 gtaaaagccc ggcgatgcaa cacctgctca gtgaaatcgc cctcgtcgcg ccatcggaag
    13621 ccacagtgct gatccacggc gattccggca ccggtaaaga gctggtcgcc agggcgattc
    13681 acgccagtag cgcacgtagc gaaaaaccgc tggtaacgct caactgtgcg gcactcaacg
    13741 aatccttgct ggaatctgaa ttgttcggtc acgaaaaagg ggcgtttact ggagccgata
    13801 aacgccggga agggcgcttt gttgaggcgg acggcggcac gctgtttctc gatgaaattg
    13861 gcgatatctc gccgatgatg caggtgcgtc tgctgcgtgc gattcaggag cgcgaagttc
    13921 agcgtgtcgg tagcaaccag actatctcgg ttgatgtccg gctgattgcg gcgacccatc
    13981 gcgatcttgc cgcagaggtg aatgccgggc gttttcgcca ggatctctac tatcgcctga
    14041 atgtggtggc gattgaagta ccatcgctgc ggcaacggcg ggaggatatc cctctgctgg
    14101 ctggccattt tcttcagcgt tttgccgagc gtaatcgcaa ggcggtaaaa ggctttacgc
    14161 cacaggcgat ggatttgctg atccactacg actggccggg aaacattcgt gaactggaaa
    14221 acgcggtgga aaggtcggtg gtgctgctga ccggggaata tatttccgaa cgcgagctgc
    14281 cgctggcgat tgccggtacg ccgatcccgc tggtacaaag tcaggatatt cagccgttgg
    14341 tggaagtgga aaaagaggtg attctggcgg cactggagaa aacgggcggc aacaaaaccg
    14401 aagccgcccg tcagttaggg atcacgcgca aaacgctatt ggcaaaactg tcgcgttagt
    14461 tctgctcgcg ttcgatagcg cgccagccga tatctttccg gcagaagcag tcgtcccagt
    14521 gaatatcggt cattaaggca taagcgcgtt tctgcgcttc tgccacggta tgacccagcg
    14581 cggtgacgca cagtacgcgc ccgccgctgg tcactacctg ctcgttatcc gccagtttcg
    14641 tgcccgcgtg gaacactttg ccgtcagcca cttcttccag cggcaggccg tggatcacat
    14701 cgccggtgcg gtaatcgccc ggatatccac ccgcagccat caccacgccg agagaagcgc
    14761 gctcatccca ctcggacgtt ttctcgtcca gcttgccttc acaggccgcc aggcagagct
    14821 caaccagatc ggacttcatg cgcagcataa tcggctgggt ttctggatcg ccaaagcggc
    14881 agttaaattc gataaccttc ggattgccct gtttgtcgat catcaggccc gcatagagaa
    14941 aaccggtgta gatgttgcct tccgccgcca tgcctttcac ggttggccag atgatacgtt
    15001 ccatggtgcg ctgatgaacg tcatcggtta ctaccggcgc gggggagtaa gctcccatcc
    15061 cgccggtgtt tggtccggta tctttatcgc ctacgcgttt gtgatcctgg ctggtggcca
    15121 tcggcagcac atgctcgccg tccaccatca cgataaagct cgcttcttcg ccatcgagga
    15181 actcttcgat aacgatgcga tgacccgcgt cgccaaaagc gttgcccgcc agcatatcgt
    15241 gaacagccgc ttccgcttct tccagcgtca tcgccacgat aacgcctttc ccggcagcca
    15301 gaccgtccgc tttaatgacg attggcgcgc ctttctcacg cagatacgcc agcgcaggtt
    15361 ctacctcggt gaagttctgg tattccgccg tagggatctt atggcgggcc aggaaatctt
    15421 tggtaaacgc ttttgagcct tccagttggg ccgcacctgc ggttgggccg aagattttca
    15481 gcccggcggc gcggaaggta tcgaccacgc ctttcaccag cggcgcttcc gggccgacga
    15541 tggtcagatc aatcttttcg ttttgtgcga aatccaacag cgccgggata tcggtcacgc
    15601 caatagcaac gttttgcagc gcgggttcca gtgcagtgcc tgcattaccc ggagcaacaa
    15661 aaacagtctc aaccagcggc gactgggccg ctttccaggc cagcgcgtgc tcgcgcccgc
    15721 cgttaccaat cactaatact ttcatctatt gctccattaa tggcggaagt ggcgcatgtc
    15781 ggtgaagagc atcgcaatac cgtgctcgtc ggcggcggca atcacttcgt catcacggat
    15841 ggaaccgcca ggctggatta cgcaggtcac gcccgcagcg gcggcggcat caataccgtc
    15901 gcggaacggg aagaacgcgt cagaagccat cgaggaacct ttcacttcca ggccttcatc
    15961 ggcagcttta ataccggcga ttttcgcgga gtacacgcgg ctcatctggc ccgcgccaat
    16021 accgatggtc atattgtttt tggcatagac gatagcattg gatttcacga acttcgccac
    16081 tttccagcag aacagcgcat cgcgcagttc ctgttcggtc ggctgacgtt gggtgacgac
    16141 gcgcagttct tccgcaccga ccatccccag gtcacgatcc tgaaccagca gaccgccgtt
    16201 cacgcgtttg aaatcaagac ccggaacacg ctcgccccac tgaccgcagg tcagaacgcg
    16261 tacgttctgc ttggcggcgg tgattttcag ggcttcttcg ctggcggaag gcgcaataat
    16321 cacttcgaca aactgacgag aaatgatggc ctgtgcggtt tccgcatcta gctcgcggtt
    16381 aaaggcaatg atgccgccga atgcggaggt tgggtcggtt ttgtacgcgc gatcgtaagc
    16441 atcaagaatg gaattgccga tagctacacc gcaagggttg gcgtgcttca caatcacaca
    16501 tgccggctcg gcgaactctt tcacgcactc cagcgccgca tcggtatcgg cgatgttgtt
    16561 ataagagagg gctttaccct gaacctgggt tgcggtagca acggaggctt ctttcacatt
    16621 ctcttctata tagaaggcag cctgctggtg gctgttctcg ccgtaacgca tatcctgctt
    16681 cttaatgaag ttcaggttca gcgtgcgtgg gaagcgacca gcggcttctt tgctttcacc
    16741 gtggtacgcc ggaaccatgc tgccgaagta gttggcaatc atgctgtcgt aggcggcagt
    16801 gtgttcgaag gctttgatgg cgaggtcgaa acgggttgca agcgtcagcg atccttcgtt
    16861 gtcatccatc tctttaataa tggcgtcgta gtcgctgctc ttcaccacga ttgcgacatc
    16921 tttatggttc ttagcggcgg agcgcaccat ggtcgggccg ccgatatcga tgttctcaac
    16981 cgcatcttcc agcgagcaac cttcacgggc cacggtctgg gcgaacggat acaggttaac
    17041 aaccaccata tcgataggct ggatctgatg ttcttccata atggcatcgt cctggccgcg
    17101 acggcccaga atgccaccat gtactttcgg atgcagggtc ttcacgcgtc catccatcat
    17161 ctccgggaaa ccggtgtaat cggaaacttc ggttaccggc agaccttttt ctgctaacag
    17221 acgggcagtg ccccctgtag acagcagctc cacaccgcgt gcggaaagtg cctgggcgaa
    17281 ttcgacgata ccggctttgt cagaaacact gagcagagcg cggcggactg gacgacgttg
    17341 ttgcatggta aatcccctgg atttgactat tacagagagc gttagctgaa tttttcgcga
    17401 aaaactcagc taacgcccct aacggggcat ccttattttt cgcccgcatt gtaacgaaaa
    17461 cgtttgcgca acgctcgcga atttttctct ttcaatggtg atcacaattt tgacagtggt
    17521 taccgtggga aaaatacaga aattacattg atgattgtgg ataactctgt gcgtaaaaag
    17581 gtataaagcg gggttttgct ggggaatgca gcagtcagtc atttttctgc aatttttcta
    17641 ttgcggcctg cggagaactc cctataatgc gcctccatcg acacggcgga tgtgaatcac
    17701 ttcacacaaa cagccggttc ggttgaagag aaaaatcctg aaattcaggg ttgactctga
    17761 aagaggaaag cgtaatatac gccacctcgc gacagtgagc tgaaagccgc gtcgc
//
LOCUS       DAZGSP010000104        17776 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZGSP010000104 DAZGSP010000000
VERSION     DAZGSP010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.105"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(204..2837)
                     /gene="mngB"
                     /locus_tag="ACHK74_003935"
     CDS             complement(204..2837)
                     /gene="mngB"
                     /locus_tag="ACHK74_003935"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFP0222670.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLLERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLFLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(2855..4771)
                     /gene="mngA"
                     /locus_tag="ACHK74_003936"
     CDS             complement(2855..4771)
                     /gene="mngA"
                     /locus_tag="ACHK74_003936"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFP0222671.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            4940..5662
                     /gene="mngR"
                     /locus_tag="ACHK74_003937"
     CDS             4940..5662
                     /gene="mngR"
                     /locus_tag="ACHK74_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFP0222672.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSAR"
     gene            complement(5766..6635)
                     /gene="sucD"
                     /locus_tag="ACHK74_003938"
     CDS             complement(5766..6635)
                     /gene="sucD"
                     /locus_tag="ACHK74_003938"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFP0222673.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(6635..7801)
                     /gene="sucC"
                     /locus_tag="ACHK74_003939"
     CDS             complement(6635..7801)
                     /gene="sucC"
                     /locus_tag="ACHK74_003939"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFP0222674.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(7895..9112)
                     /gene="odhB"
                     /locus_tag="ACHK74_003940"
     CDS             complement(7895..9112)
                     /gene="odhB"
                     /locus_tag="ACHK74_003940"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFP0222675.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(9127..11928)
                     /gene="sucA"
                     /locus_tag="ACHK74_003941"
     CDS             complement(9127..11928)
                     /gene="sucA"
                     /locus_tag="ACHK74_003941"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFP0222676.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(12229..12945)
                     /gene="sdhB"
                     /locus_tag="ACHK74_003942"
     CDS             complement(12229..12945)
                     /gene="sdhB"
                     /locus_tag="ACHK74_003942"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFP0222677.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(12961..14727)
                     /gene="sdhA"
                     /locus_tag="ACHK74_003943"
     CDS             complement(12961..14727)
                     /gene="sdhA"
                     /locus_tag="ACHK74_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFP0222678.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(14727..15074)
                     /gene="sdhD"
                     /locus_tag="ACHK74_003944"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(14727..15074)
                     /gene="sdhD"
                     /locus_tag="ACHK74_003944"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFP0222679.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(15068..15472)
                     /gene="sdhC"
                     /locus_tag="ACHK74_003945"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(15068..15472)
                     /gene="sdhC"
                     /locus_tag="ACHK74_003945"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP0222680.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFI
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            15853..15960
                     /locus_tag="ACHK74_003946"
     CDS             15853..15960
                     /locus_tag="ACHK74_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222681.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16166..17449
                     /gene="gltA"
                     /locus_tag="ACHK74_003947"
     CDS             16166..17449
                     /gene="gltA"
                     /locus_tag="ACHK74_003947"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFP0222682.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT         4256 a         4809 c         4383 g         4328 t
ORIGIN      
        1 ccacatatac gtggcttgag agtgaagata aacgcaaaat tacctgatgc gctacgcttg
       61 ccagtgtcta catcatctct gcaatatatt gaatttgcct gcttttgtag accgaataaa
      121 gcgttcatgc cgtatccggc atgaacaaag cgccctttgt caacaatctg gccgcgcata
      181 tgtggcttcg gtttgattgc tattcaggca agccggtaac tgaacgtccg tgactggccg
      241 ggtaaaaaag gccccgatag atttgaacct tgattttcct cggtggtaat gtgttcatcc
      301 atcatcgttt ctgagcaaga aatcacctcg cgactgaaag caacagtcgc atcacaggtt
      361 gctgattcag ccggattaaa cagccgtaaa atcacttctt gtcggtcttc agctttctta
      421 agtgcgctta tcaggcatcc cactgggggc attttcaaca aactataact ttccggcacg
      481 ttgaatccgg ctttgttgag cttcatcgca tcccatggga ttttgttgta acactgtact
      541 ggagtcagcc atgctcgcgc ctgctgagct acaccagcgg cggttggcgt accggtataa
      601 ctcaataaac ttaggcgaca agaaagcaga ccacgtagtt gtgagtccgg gactggcatt
      661 ttaattcccg aaggccgccc aggccttaaa aacagatctt ctttgcccag taagcccacg
      721 ccacgcagca acgtaatggc aaaggttttc ttctcttcac cgatgacttc aaattcacgt
      781 agcccttcgc taaagacagc catgccgtta cgcccttcct gtaaggctgc atagttgagc
      841 atattccata ccggaaccgg cgcttctttc cagccttctt gctgccagtt gttcattgca
      901 ctgtcgttca cggggcgcgt tagcgaacca aactgggtat ctgccagaac actatcggtg
      961 ttaaaagatg tagggatcag gacgcgaagg cgatgatcgt cagcctggtt atcaaggttg
     1021 atatccacat caatacgtct gctgttatga ctcagagtga caaccatctc cacccctact
     1081 ctgcccgtgg attgccggac gcttcgttcc aacaaattga gcggcaccgc catgtcatag
     1141 cggataacag ccctgctctg ccaggcctca tgagtaatct cgcattgcgg tttcgctgtt
     1201 gcagaagtca ttacccactc ttcttttgcg ggtgaatagt catattcatc accatcatca
     1261 gagctttctt caatttgcaa cacccggtca tagcgcacac cgctatcttt atctaccagt
     1321 tgtagcgttc catcctcatt gagcgcaatt tgccagaaag cattttccag tatcccttca
     1381 gcgtcacttt ttgccgcaat cacgttgcca ggctgattcg cttcgatata aagcgtgcga
     1441 tagcccatag aagggacaat ctggttgatc tgtatatcaa actccataaa gggatcgtaa
     1501 ttaccgtaat gaactatttg ccgatcgatt aggcctggat cgatctcacg ggcatggcga
     1561 ataaaatacg gtacaggctg accgcgatcg tctcgtagat taaactggct ggcgcgcagc
     1621 cgcacagtgg tgttaataac ttcttcccgc ggccagggca tcaggttaaa cagcacgagt
     1681 ttgtcggcat cactctgtgg catgttatcg gcaattttgc gcatgtagaa acgcagcaga
     1741 ttatccgcca tgtcttcagc cagttcgaag cgggcgacaa tttcgcgatg aactttgtca
     1801 ctgcaacaac aaccgatact gtcgtgggca tgatttttta agatctcttt ccacattttc
     1861 tccagcaagc cgtggtggta ttcaaaaccc aacgtccagg ccagcgttgc cagcggttcc
     1921 agcagattaa caatcttatt ttcaatacgt gcgtgggcaa ttttgatatc catgcgcgtg
     1981 gaaccgatgg tgcgatgtac gcgcatatat ttgccatcga taaattcacc tttcagggtg
     2041 gccagacttt cacgctgcgc ttcgatcttc tcaaatacct cttcaaagcg gctcatcaca
     2101 aacttacgtt gagggtagat ctcacgtagc ttatccatca cttcgaagat attttgctgc
     2161 aatggcatct ggtcatgccc attcggcagc aaaatctctt tggttacaga cgctttttcc
     2221 agcacgtcaa aataactgtc gaggcgttta cgtaatccgt tttcgtcggc aggtaagtac
     2281 ttaccgatgg cgtagcccag cggcagcacc tgcgccgtca cttcgctacc gtcactgctt
     2341 tgccacaaaa actcggtttt atcagtaccg tggcgctccg aacatccgcg ccagaacatg
     2401 gtgcgggtaa tgccaaatcc attgtagata tgcggcagtt gcccggacat gccaaaggaa
     2461 tctggtaaat aacctatttt catcggctcg ccaaacgcga ggcagtcacg cattccgtac
     2521 atcagattac ggacgatgga ttccgcagaa acaatcgtgg tatcggtctg ggtataccag
     2581 gggccgataa tcaacttgcc ggcttctacc tgtttcttca cgcggtcttt gttttccggt
     2641 ttcaccgcga aataatcttc gaggatcgcc gtttgcccgt cgagtacgta atatttgtat
     2701 tcgttgtcct gttccagtcg gcacaggatc tcttccatat tattgaccag cagaatgcgt
     2761 gactcttcgg tggtgaaata ccactcccga tcccaatgca tatgcggggt gatgtgaacg
     2821 cgagataccg ctttcatcgt cgtttccttt ttgtttatgg cattacgcca tcagtcagat
     2881 agttgccatg cttaaccgcg tgacgccgcc agatcaggag aattgcagtc gagattgcag
     2941 cccccaccaa tgccgcacca aaccagccaa ttgccgccat aacaccgccc gcgccattat
     3001 catgaagtaa aaagagcgag aaaatgccgg caccgggtgt cgaaaggccg atattcatcg
     3061 caccgacaat agcgcccgtt accatagagc ccagcacaaa cgaaccaata acccgcagcg
     3121 gatcttcaat cgccatcggg atcgcccctt cggtaatacc tgccagccct aacagccagg
     3181 tggatttccc ggtctcaatt tcaaactctt taaacaggcg cggtgcgagc atcgtggaag
     3241 cggttacggt aaatgccgaa accattttga cggaggcgaa aatggcatac gggccgtaaa
     3301 cgccgttcgc cattgcgccc aggcagaatg cataagcggc tttgttcact ggccctccaa
     3361 ggtcaaagga acacataaaa ccgagaatgg cacccagcaa cagcgcgtta cttcctgaca
     3421 gaccgttcag ccaggcggta agcgagttat tgatccaggc gacaggttct cccaccacaa
     3481 acagcatcag actgcccgct cccaacgtac cgagcaccgg gtagagataa aaagtcagga
     3541 atccattgaa tttactgcta agacgcaagt gatttttcac ccagcgcatc aagtaaccgg
     3601 ctatcaatcc gccaacgacc gcgccgagaa acccggagcc gatcatgttg gcggcaagtc
     3661 cagccgcaaa gcctggcgct aacgccggtt tatctgccag agaataggcg gtataggccg
     3721 cgagcaccgg taccatcaaa attccgagca gcccgccgcc cagcttgcgg tacatccaca
     3781 gccaggagtt ttcttcatta aacagatctt gtagcccgaa gatttgcgac agtaataccg
     3841 cgaccgccag caccgtgccc cccgcgacaa tcaacggtac ggcaaaagag attccgctca
     3901 acagtgcctg tttcagctcc gttttgacac ttttcaccgg ttgcgtatct tgctgtacgg
     3961 tacgcgtctc atcgctacgc ttgagggtaa gcgcttgttg gatcaacgct tctgcatggc
     4021 gaatcggctc ggcaacaggc actgaaagcg cgggaatgcc attaaaacgc tcactctcct
     4081 tgatggcgac ttcagccgca aaaatacagg cggttgcact attgagttga tccgccgtta
     4141 aacgcccttc aatgccgtta gcgccttgtt tttcaacgta aacatttacg ccgagtttgc
     4201 gtccggcttt ttccagatat tccgcagcca tataggtgtg agcaataccc gccggacagg
     4261 ccgttacgca gacgatagtt ggcgcgttgg aaaaagaggc agaaggttgc gtgcctccct
     4321 tgtcatccag cgccgagagc aactcatcag gcgtcgtcgc cgactgtata cgcgcccgaa
     4381 tctcatcatc cgccaggcgc gtggtcagcg ctgtcagcag ttgcatatgc gtagtacccg
     4441 cttcattggg gggaatcgcc agcagcacca ctaaatcaac cgcttccggg ccatcaacgc
     4501 cttcccactg aagcggctcg ctgagtgtgg cgaccgcaaa cgccgcttct tttaccgcag
     4561 cagttttgcc atgcggcaca gccaaccctt cacctaaggc ggtcgggcca aggctttcac
     4621 ggcgatacac ttcttccaga aattgctcag tactggaaat tttccccaga gcagcaagac
     4681 gttgagtcaa cgcgtggatg gcctcttcac ggctggtaaa gcgcgcattc agacacaacg
     4741 catcgcggtg ggtcagagtc gtcagattca tcatgatcct cacttgatcg tttttatagt
     4801 cgcgccagtg tgcccgataa aacaatacca ttatgtgatc aatttcactt gattgaaatt
     4861 atttgtattg tatttgtatt atttacagga tgaatttatc gggcataatg gctaaattcg
     4921 tattaatgag atactaaaaa tgggacacaa gcccttatac cggcagattg ccgatcgcat
     4981 tcgtgaacaa attgcgcgtg gcgagcttaa acctggcgat gcgttaccta ccgaatcggc
     5041 attacaaaca gagtttggcg tcagccgggt tacggtgcgt caggctttgc gccagttagt
     5101 cgagcaacag atcctcgaaa gcattcaggg cagcgggact tacgtcaaag aagagcgggt
     5161 caattacgat atttttcagt taaccagttt tgatgaaaaa ctgtcggacc gtcacgtcga
     5221 tacacacagt gaagtcctga tatttgaagt gattccggct gacgattttc ttcagcaaca
     5281 gctacaaatc accgcgcagg atcgtgtgtg gcatgtgaag cgcgtgcgct atcgcaagca
     5341 aaagccaatg gcgctggaag aaacctggat gccgcttgcg ttgttcccgg atctcacctg
     5401 gcaggtcatg gaaaattcaa aatatcactt tattgaagaa gtgaagaaaa tggttatcga
     5461 tcgtagcgaa caggaaatca ttcctctgat gccaaccgaa gagatgagcc gtctgcttaa
     5521 tatcagccag acaaagccga ttcttgaaaa agtatcgcgt ggatatttag ttgatggtcg
     5581 ggtgtttgaa tatagccgca acgcttttaa taccgatgac tataaattta ccctgatagc
     5641 gcaacgaaaa tcagcgcgat aagcacaaaa aaggccatca tacgatggcc aaccatgtcg
     5701 aaaccgacag cccttcgcca ttcctgtaga gggaagcggc gagggctatt tcttattaca
     5761 gatatttatt tcagaacagt tttcagtgct tcaccgatat ccgccaggct gcgaacggtt
     5821 ttcacgcctg cggcttccag agcagcgaat ttctcatccg cagtcccttt cccaccggca
     5881 atgatggcac ccgcgtggcc catacgtttg cctttcggcg cagtcacacc agcgatgtaa
     5941 cccacaactg gcttggtaac atgctctttg atgtacgctg ccgcttcttc ttcagcgcta
     6001 ccgccgatct caccgatcat tacgatcgct tcggtctgcg gatctttttc gaacatttcg
     6061 agaatgtcga taaagttaga gccagggatc gggtcaccgc cgataccaac acaggtcgac
     6121 tgaccgaaac cgtaatccgt ggtctgttta accgcttcat aggtcagtgt accggaacgg
     6181 gaaacgatac ccactttacc cggtttgtga atgtgacccg gctggatacc gattttgcat
     6241 tcacccggag tgataacgcc cgggcagttc gggccgatca tacgaacgcc ggcttcgtcc
     6301 agtttcactt tcacagtcag catatccagc gtcgggatgc cttcagtgat ggtgataatc
     6361 agtttgatgc ctgcatcaat agcttccaga atggagtctt tgcagaacgg tgctggtacg
     6421 tagataacag aagcggtagc gccagtagca gcaacagctt cacgcacggt gttgaacacc
     6481 ggcaggccga ggtgggtagt accgccttta cctggggtta cgccgccaac cattttagtg
     6541 ccgtatgcaa tggcctgttc tgagtggaaa gtcccctggc taccagtaaa gccctggcag
     6601 ataaccttgg tgtttttatc aattaaaatg gacattattt cccctccact gcggcaacaa
     6661 cctgctgagc tgcatccgtc agaccttttg ctgcaataat attcaggccg ctgtcagcca
     6721 gtttcttcgc gccgagttcg gcgttgttac cttccagacg taccacgacc ggtacgttaa
     6781 cacccacttc tgctaccgcg ccgatgatac cgtcagcgat caggtcgcaa cgaacgatac
     6841 cgccgaagat gttaaccaga acggctttca ctttgtcgtc agagaggatg attttgaacg
     6901 cttcggttac acgttctttg gttgcgccgc cgccaacgtc aaggaagtta gccggttcgc
     6961 cgccgtgcag tttaacgatg tccatcgtac ccatcgccag acctgcgccg ttaaccatac
     7021 aaccgatgtt accgtccagc gcaacgtagt tcagttccca ctgtgcagcc tgtgcttcac
     7081 gcggatcttc ctgcgactgg tcacgcattt cgcgcagatc aggctggcgg aacagtgcgt
     7141 tgccgtcagc gcccagtttg ccgtcgaggc aaatcagatc gccctgtttg gtgatgacca
     7201 gcgggttgat ttcgatcaac gccaggtcgc gctccaggaa aatggtcgcc aggcccatga
     7261 agattttggt gaactgctga accagtttac cttccagacc cagtttgaac gccagctcgc
     7321 gtccctgata cggcatcggg ccagtcagcg gatcaagcgc aactttgtgg atcaggtgcg
     7381 gagtttcttc cgccactttt tcgatttcca cgccgccttc ggtggaggcc ataaagacca
     7441 cacgacggga actacggtct acaacggcac cgagatacag ctctttagcg atatcggtcg
     7501 ctgcttcaac cagaatctgg ttaaccggtt ggccattggc atctgtttga tacgttacca
     7561 gacgcttgcc gagccagttt tctgcaaaag cacggatgtc ttctttgctg tttacaactt
     7621 tcacaccgcc cgctttaccg cggccaccag cgtgaacctg acatttcact acccacgggc
     7681 cggcaccgat ttttgaagcg gcttcttctg cttcgcgcgg agtagtacag gcataaccca
     7741 ccggtgctgg taagccatag cgggcaaaaa gttgttttgc ctgatattca tgtaagttca
     7801 tgtgttctgt ccatccttca gtaatcgtta tcttttaaac cgtagaccga caccctatac
     7861 acgtcggtct acagtgcagg tgaagcttaa actactacac gtccagcagc agacgcgtcg
     7921 gatcttccag caactctttg atcgttacca ggaagcccac ggattcgcga ccatcgatca
     7981 gacggtgatc gtaggacagc gccaggtaca tcatcggcag gatctcaacc tgaccattca
     8041 ccgccatcgg acgatctttg atagcgtgca tacccagaat tgcgctctgc ggcgggttga
     8101 tgatcggcgt agacatcagg gaaccgaaca caccaccgtt ggtgatggtg aagttaccac
     8161 cggtcagatc ttcaacggtc agcttgccgt cacggccttt gactgccagc tctttgattt
     8221 tcttctcgat gtctgccatg ccgagggtat cgacatcacg cagtaccggt gtcaccaggc
     8281 cgcgcggcgt agaaaccgcc atgctgacgt cgaaatagtt gtggtaaacc acgtcatcgc
     8341 cgtcgataga cgcgttcact tccgggtaac gtttcagggc ttcaaccacc gctttcacgt
     8401 agaaggacat aaagcccaga cggatgccgt ggcgtttttc aaacgcttca ccgtactgct
     8461 tacgcagatc cataatcggc ttcatgttga cttcgttgaa cgtggtcagc atggcggtgg
     8521 agtttttcgc ttccagcaga cgctctgcca cacgcttacg caggcgagtc atcgggacac
     8581 gtttttcact acgtgcagcc agagccggtt gcgccgccgg agcagccgct gccggagcag
     8641 actctttcgc cggggctttc gccagatgtt tttccacatc ttcacgagtc agacgcccac
     8701 ccacaccggt gcctttaatg gcgctggcgt cgagattgtg ttcagccagc aggcgacgga
     8761 tcgccgggct taacgcatcg ttgttttgct cttccagaga tgcctgctgg cgttgcgccg
     8821 gagtggacgc tttctcttca gatttggcgc tggtttcttt accggcgctg ttgccttcac
     8881 gcaggcgacc aaggatctga cgagacgtta ccgttgtacc ttcatcttcc agaaccgcat
     8941 ccagaatgcc gtctgctgat gccggtactt ccagtaccac tttgtcagtt tcgatttcta
     9001 ccagcacttc atcacgtacg actgcgtcgc cgggtttttt atgccaggtt gcgacggtgg
     9061 catcggctac ggattcaggc aggtcaggga ccagaatatc tacgctactc attgtgtatc
     9121 ctttatttat tcgacgttca gcgcgtcatt aaccagatct tgttgctgtt tctggtgaac
     9181 ggacatatac cctaccgccg gagaggcgga ggccgggcgg cctgcataac gcagagaagc
     9241 cccaaacgga atcacttcac ggaaatgatg ctggctgcag taccatgcgc cctggttgag
     9301 cggctcttcc tggcaccaga caaaatcctt gacgtgagca aactgctgca acacttcctg
     9361 catcgcttta tgcgggaacg ggtagagttg ctcgatacgc acaatggcga catcgtgttg
     9421 attgttctta cgacgctgtt ccagcaggtc gtaataaacc ttaccagaac acatcactac
     9481 gcgcttcacg cccttcggat caagctcgtc gatttcaccg atggctggca ggaaggtgcc
     9541 gttcgccagt tcttcgaggc tggaaaccgc cagcggatga cgcagcaggg atttcggcga
     9601 catcacgacc agcggacgac gcatcccgcg cagcgcctga cgacgcagca tgtggtaaac
     9661 ctgtgccggg gtagacggta cgcacacctg catgttttgc tcagcacaaa gttgcagata
     9721 acgttccaga cgcgcggagg agtgctccgg cccctgccct tcgtaaccgt gcggcagcaa
     9781 catcaccaga ccacacatcc ggccccattt ctgctcgcca gaggagatga actggtcgat
     9841 aaccacctgc gcaccgttgg cgaagtcacc gaactgcgct tcccagatag tcagagtgcg
     9901 tggttctgcg gtggcataac cgtattcaaa cgccagcact gcttcttcag acagaacgga
     9961 gtcccagaca cggaacgcgc cctggccgtt atgaatatgt tgcagcggcg tgtaagtgga
    10021 accgttagac tggttgtgga tcaccgcgtg gcggtggaag aaggtaccgc gaccggagtc
    10081 ttcacccgac aggcgaaccg gaatgccttc gtcaaccagc gtggcgtaag cgaggttttc
    10141 cgcaccgccc cagtcgaaca gtttctcacc ggcagccatc gcctggcgat cgccataaat
    10201 cttggcaacg cgagactgca tttcaactgc ttccggcacc gtgctgatgc gtttcgccag
    10261 ctcctgcagg cgcttcatct caactttgtt cgggtactct tcgtcccatt cgtggttgag
    10321 gtacggcgac caggtgaaag agtgcatgtt catcggacgc cactctgcca ctacgcaatc
    10381 gccagcatcc agcgcatcgc ggtacaggtt aaccatctcg gtggcatctt ccagcgtcgc
    10441 gactttttcc tgctccagct tgtcagcgta gattttgcgc ggtgtcggat gttttttgat
    10501 tttctgatac atcagcggct gggttgcgct cggctcgtcg gcttcgttgt ggccgtgacg
    10561 gcggtagcac accaggtcga tgaagacatc acgtttaaag gtgttacgga aatcgagcgc
    10621 cagacgggtc acaaaggcaa cggcttccgg atcatccgcg ttaacgtgga aaatcggtgc
    10681 ctgaaccatc ttaccgatat cagtacagta cggtgtagaa cgagcatcca gcggattaga
    10741 ggtggtgaaa ccaacctggt tgttgataac gatacgtacc gtaccgccaa cttcataacc
    10801 acgcgctttc gacatgttca gagtttcctg aaccacgccc tgcccggtca ctgcggcgtc
    10861 accgtggatg gtgattggca gcactttgtt gctgctcggc tcatcaagtc tgtccagacg
    10921 ggcacgaaca gaaccgataa ctaccgggct tacaatctca aggtgagacg ggttaaacgc
    10981 cagcgccagg tgcaccaggc cgccatcggt ctggaagtca gacgagaagc ccatgtggta
    11041 tttcacgtca cccgtgccga ggtgttcttt atgtttaccg gcaaactcgt cgaacaagtc
    11101 ttgcggtttt ttacccagca cgttcaccag cacgttcaga cgaccacggt gcgccatccc
    11161 aagaaccact tcgcgggtgc cgctgttgcc agcgtggcgg atcatctctt taagcatcgg
    11221 gattaacgcg tcaccgcctt ccagcgagaa gcgttttgcg ccagggaatt ttgcgccgag
    11281 gtaacgttca aggccttcag cggcggtcag ttcgcttaag aagcgttttt tctcttcgct
    11341 attgaaagtc gcgcgaccag actcgatacg ctgttggatc cagcgttttt cttcggtgct
    11401 ggtaatgtgc atatactcgg caccaatcgg gccacagtag gtttgcttga gggcttccag
    11461 cagctcgccg agtttcatgg tttctttgcc gctggcaaat gaaccgacgt tgaaggtttc
    11521 ctggaagtct gcttcggtca gatcgtggaa agacggatcc agatcggcca ctttatcttg
    11581 ctgccacagt cccagcggat cgagattcgc atgctggtga ccacggaagc ggtatgcgtt
    11641 aatgagctgc aggactttaa cctgcttcac attggtgtca gggtcggaga tcgttgaaga
    11701 gtaacgtgaa gcgtctttcg ccaggcggcg gaaatattca cgcgtttgag agtggaattg
    11761 atccggtttg actcccgtac caggtaactg ctggaacgtc gaacgccagt tagcgtcaac
    11821 cgagtcagga tcggttaaga agtcttcata gagctgttct atccagctct ggtttgcgcc
    11881 agagaggtaa gaagagtcca accaggcttt caaagcgctg ttctgcatcg tgatccctta
    11941 agcatctttt ttatgcttac ttcgccgtgg atactaccac gcacagtgat gtgcgtgccg
    12001 gggttcactt gcgagctctt gtctatgtag tggctcgcga aggaaccttt aaaaactgtc
    12061 ttagtgtcag gggcagtttt taaagatttc ctgcatttct ttgttacgcc tgatgcgctg
    12121 cgcttatcag gcctacgggt aatgacttgt aggccggata aggcgttcac gccgcatccg
    12181 gcactggttg cctgatgcga cgcttgcgcg tcttatcagg cctacggttt acgcattacg
    12241 ttgcaacaac atcgacttaa tatggccgat ggcgcgcgtc gggttcagcc ccttcggaca
    12301 tacactgacg cagttcatga tgctgtgaca gcggaatacg ctgaatgcat cgctcaaacc
    12361 gtcgaggcgg ctgtcagtct cggtatcacg gctatcgatc aggaaacgat atgccgctaa
    12421 caagcctgcc gggccgataa acttatcggg attccaccag aaagacggac aagaggttga
    12481 acaacatgcg cagagaatac attcatacag cccgtcgagt ttttcgcgct gctctggcat
    12541 ctgtaaatgc tcgcgagctg gcggattttg tccattattc aacaggtaag gcttaatttt
    12601 ctcatattgc gcatagaatt gtcccatgtc taccaccaaa tcgcggatca ccggtaaacc
    12661 tggcagcggg cgaatcacaa tcttcttgcc cggctggttg agtgccgaaa tcggggtaat
    12721 acaggccaga ccattcttgc cgttcatgtt cagaccgtcg gaaccgcaca caccttcacg
    12781 gcaggagcgg cggaacgaca ggctgggatc tttctctttc agctggataa gcgcatccag
    12841 cagcatcatg tcgcgacctt cttccgcttc cagggtgtaa tcctgcatac gcggagcatc
    12901 atcaacatcc gggttatagc gataaattga aaactcgagt ctcattttcc tgtctccgca
    12961 ttagtaagta cgaatcttcg gcgggaatgc cgggcgcagt ttcggttcca tgttgacgct
    13021 tcggcgcgtc atggattccg actctggcag atacagggag tggcacagcc agttttcatc
    13081 atcacgatcc gggaagtcga agcggctatg tgcgccacgg ctttcggtac ggaagttggc
    13141 agaaacagcc gttgcatacg ccgtttccat caggttatcc agttccaggc actcaacgcg
    13201 ctgggtgttg aactcgctgg aagtgtcatc cagacgggca tttttcagac gctcgcggat
    13261 cactttcaac tgctcaagcc ctttcgccat cgcatcacct tcacggaaga ccgagaagtt
    13321 atgctgcata cattcttgca gcgctttacg aatcgccacc ggatcttcac cgttacgatt
    13381 attgttccag cggttcagac gatccagaga tgcttcaaca tcagactcgc tggcatcgcg
    13441 caatgcgccc tgttcggcga tagactcttg cagatgcaga cccgctgcac gaccaaagac
    13501 caccaggtcc agcagcgagt tgccgcccag acggttagcg ccgtgtaccg atacacaagc
    13561 gatttcacca acggcaaaca gtcccggaac aaccacatct tcgcctttct cgttcaccgt
    13621 cagcgcctga ccggtcactt tggtcggaat accgcccatc atgtagtgac aggttgggat
    13681 aaccggaatc ggctctttca ccggatcaac gtgagcgaag gtacgggaga gttccaggat
    13741 acccggcagg cgggattcga gaacttcttt acccaggtgg tcgagtttca gtttcgcgtg
    13801 tggcccccac ggaccatcac agccgcgacc ttcacggatt tcgatcatga tggaacgcgc
    13861 caccacatca cggcccgcca ggtctttggc gttcggcgca taacgctcca taaagcgttc
    13921 gccatgtttg ttcagcagat aaccgccttc accacggcaa ccttcggtga ccagtacgcc
    13981 cgcaccggca ataccggtcg ggtggaactg ccacatttcc atatcctgca ccggtacgcc
    14041 tgcgcggata gccatgccga cgccgtcgcc agtgttaatg tgggcgttag tggtggactg
    14101 ataaatacgc cctgccccgc cagtcgccag cacggtagcg cgggctttga aataaaccac
    14161 ttcaccagtt tcgatgcaca gtgcagtaca acccaccacc gcgccatcct ggtttttcac
    14221 cagatccagc gcataccact cggagaaaat agtggtgtgg tttttcaggt tctgctgata
    14281 aagggtatgc aacagtgcgt gaccggtacg gtcagctgct gccgcagtgc gtgccgcctg
    14341 ctcgccgccg aagtttttcg actgaccgcc aaacggacgt tgatagatac gaccatcatc
    14401 aagacgcgag aacggcaggc ccatgtgttc gagttccaga atcgcttccg gcccggtttt
    14461 acacatatat tcaatcgcgt cctggtcacc gatatagtcc gaacctttta cggtgtcgta
    14521 catatgccat tcccagttat cttcatgggt attacccagc gcaacggtaa tgccgccttg
    14581 cgcagaaacg gtatgggaac gggtcgggaa gactttagaa agcagcgcac aggtttgacc
    14641 gctctgggaa atttgcagcg ccgcgcgcat acctgcgcca ccggcaccaa tcacaactgc
    14701 atcaaattct ctgactggca atttcatcac acaccccaca ccacaacgaa tccataaatc
    14761 acgtaaacca ccagagcaac gacaatcacc agttgcagca tcaggcgcaa ggccagcggt
    14821 ttaacgtagt cggtcaacac ctgccacatg ccgatccagg catggatcaa gatagaaaac
    14881 agcgccagca gggtgaacac tttggtgaac gcagaggcga agaaaccgat ccagacttca
    14941 tatgtcagct cgccactggt agcgaaaaaa ccgaccatat aaatgatgta gagcgtcagg
    15001 acgatagcgg tagcgcgaac gaggatgaaa tcatgtacgc cattgcgtcc taatgcggag
    15061 gcgttgctta ccatacgagg actcctgcga gaagtgaaag cacgacagta ataacaaagg
    15121 agattttggc ggagcgttta cccgcttcga atgtttcttc cagatagcca aaatccatca
    15181 tcatgtggcg aatacctacg acgacgtgat acgccagagc ggtaaggatg ccccacatga
    15241 taaatttgac gaagaagctg cccataatcg cggaagcttg ctcgaaacct tcaggggaag
    15301 agaggctggt acccagaagc cacagcagga tacccactgc aataaaggtg atcacaccgg
    15361 aaacgcgatg gagaatggac gctatcgccg tgatggggaa ccggatggtc tgtaggtcca
    15421 gattaacagg tctttgtttt ttcacatttc ttatcatgaa taacgcccac atgctgttct
    15481 tattattccc tggggactac gggcacagag gttaactttc tgttacctgg agacgtcggg
    15541 atttccttcc tccggtctgc ttgcgggtca gacagcgtcc tttctataac tgcgcgtcat
    15601 gcaaaacact gcttccagat gcgaaaacga cacgttacaa cgctgggtgg ctcgggattg
    15661 cagggtgttc cggagacctg gcggcagtat aggctgttca caaaatcatt acaattaacc
    15721 tacatatagt ttgtcgggtt ttatcctgaa cagtgatcca ggtcacgata acaacattta
    15781 tttaattttt aatcatctaa tttgacaatc attcaacaaa gttgttacaa acattaccag
    15841 gaaaagcata taatgcgtaa aagttatgaa gtcggtattt cacctaagat taacttatgt
    15901 aacagtgtgg aagtattgac caattcattc gggacagtta ttagtggtag acaagtttaa
    15961 taattcggat tgctaagtac ttgattcgcc atttattcgt catcaatgga tcctttacct
    16021 gcaagcgccc agagctctgt acccaggttt tcccctcttt cacagagcgg cgagccaaat
    16081 aacaaacggg taaagccagg ttgatgtgcg aaggcaaatt taagttccgg cagtcttacg
    16141 taataaggcg ctaaggagac cttaaatggc tgatacaaaa gcaaaactca ccctcaacgg
    16201 ggacacagct gttgaactgg atgtgctgaa aggcacgctg ggtcaagatg ttattgatat
    16261 ccgtactctc ggttcaaaag gtgtgttcac ctttgaccca ggcttcactt caaccgcatc
    16321 ctgcgaatct aaaattactt ttattgatgg tgatgaaggt attttgctgc accgcggttt
    16381 cccgatcgat cagctggcga ccgattctaa ctacctggaa gtttgttaca tcctgctgaa
    16441 tggtgaaaaa ccgactcagg aacagtatga cgaatttaaa actacggtga cccgtcatac
    16501 catgatccac gagcagatta cccgtctgtt ccacgctttc cgtcgcgact cacatccaat
    16561 ggcagtcatg tgtggtatta ccggcgcgct ggcggcgttc tatcacgact cgctggatgt
    16621 taacaatcct cgtcatcgtg aaattgccgc gttccgcctg ctgtcgaaaa tgccgaccat
    16681 ggccgcgatg tgttacaagt attccattgg tcagccattt gtttatccgc gcaacgatct
    16741 ctcctatgcc ggtaacttcc tgaatatgat gttctccacg ccgtgcgaac cgtatgaagt
    16801 taatccgatt ctggaacgtg ctatggaccg tattctgatc ctgcacgctg accatgaaca
    16861 gaacgcctct acctccaccg tgcgtaccgc tggctcttcg ggtgcgaacc cgtttgcctg
    16921 tatcgcagca ggtattgctt cactgtgggg acctgcgcac ggtggtgcta acgaagcggc
    16981 gctgaaaatg ctggaagaaa ttagctccgt taaacacatt ccggaatttg ttcgtcgtgc
    17041 gaaagataaa aatgattctt tccgcctgat gggcttcggt caccgcgtgt acaaaaatta
    17101 cgacccgcgc gccaccgtaa tgcgtgaaac ctgccatgaa gttctgaaag agctgggcac
    17161 caaagatgac ctgctggaag tggctatgga gctggaaaac atcgcgctga acgacccgta
    17221 ctttatcgag aagaaactgt acccgaacgt cgatttctac tctggtatca tcctgaaagc
    17281 gatgggtatt ccgtcttcca tgttcaccgt cattttcgca atggcacgta ccgttggctg
    17341 gatcgcccac tggagcgaaa tgcacagtga cggtatgaag attgcccgtc cgcgtcagct
    17401 gtatacagga tatgaaaaac gcgactttaa aagcgatatc aagcgttaat ggttgattgc
    17461 taagttgtaa atattttaac ccgccgttca aatggcgggt taatttttat atgcctaaac
    17521 acaaaaaatt gtaaaaataa aatccactat cggacattca tatattctaa aaaaaaatat
    17581 atcggtataa attataaaca aatcgatgat ttttaatcat ctatcttcct ctcagttaca
    17641 tgaataaacc accaaatata gtgcacccct ataatttcca atactttatt catcaaacat
    17701 ttaattcata ccatcaatta attactttta catttaataa aattaataat atttttaaga
    17761 aaaactaaaa acattc
//
LOCUS       DAZGSP010000105        17729 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZGSP010000105 DAZGSP010000000
VERSION     DAZGSP010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.106"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            73..345
                     /locus_tag="ACHK74_003948"
     CDS             73..345
                     /locus_tag="ACHK74_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFP0222683.1"
                     /translation="MALPRITQKEMTERKQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(478..1350)
                     /gene="rluF"
                     /locus_tag="ACHK74_003949"
     CDS             complement(478..1350)
                     /gene="rluF"
                     /locus_tag="ACHK74_003949"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFP0222684.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1562..2251
                     /gene="pepE"
                     /locus_tag="ACHK74_003950"
     CDS             1562..2251
                     /gene="pepE"
                     /locus_tag="ACHK74_003950"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFP0222685.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(2342..3973)
                     /locus_tag="ACHK74_003951"
     CDS             complement(2342..3973)
                     /locus_tag="ACHK74_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFP0222686.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(4193..7876)
                     /gene="metH"
                     /locus_tag="ACHK74_003952"
     CDS             complement(4193..7876)
                     /gene="metH"
                     /locus_tag="ACHK74_003952"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFP0222687.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLSIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFPNYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAD"
     gene            8076..8900
                     /gene="iclR"
                     /locus_tag="ACHK74_003953"
     CDS             8076..8900
                     /gene="iclR"
                     /locus_tag="ACHK74_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFP0222688.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            9217..10791
                     /locus_tag="ACHK74_003954"
     CDS             9217..10791
                     /locus_tag="ACHK74_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFP0222689.1"
                     /translation="MITRIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNGRHIFSNKDFVIKFSTSVLQADKKEITIINKNENTTLTQTIAPI
                     FEEYLMEILPQRSDTLDKQELNLKSDRKEKEFPRIKLNGQCYFPGRPQNRIVCRHIAA
                     QYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALVSSRPEGRCVAACGDFGL
                     VMKAYFDKMESNGISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPNVTNDKI
                     RIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLMLKGSVDE
                     ITPPLSPATMNLLMAIGQNHQLTQLMIQLQKMPELHRTEMLTAYNSGHMNVINTIFNA
                     LPTLFNTFKFDKKNMKPLLLANNSNEYPGLFSAIQHKQQNVVETVYLALSDHARLFGF
                     TAEDIMDFWQHKAPQKYSAFELAFEFGHRVIAELILNTLNKMAESFGFTDNPRYIAEK
                     NYMEALLKKASPHTVR"
     gene            complement(10760..12496)
                     /gene="aceK"
                     /locus_tag="ACHK74_003955"
     CDS             complement(10760..12496)
                     /gene="aceK"
                     /locus_tag="ACHK74_003955"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFP0222690.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(12777..14081)
                     /gene="aceA"
                     /locus_tag="ACHK74_003956"
     CDS             complement(12777..14081)
                     /gene="aceA"
                     /locus_tag="ACHK74_003956"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFP0222691.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIDPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(14111..15712)
                     /gene="aceB"
                     /locus_tag="ACHK74_003957"
     CDS             complement(14111..15712)
                     /gene="aceB"
                     /locus_tag="ACHK74_003957"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFP0222692.1"
                     /translation="MTEQATTTDELAFIRPYGEQGKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADMAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(15981..16910)
                     /gene="metA"
                     /locus_tag="ACHK74_003958"
     CDS             complement(15981..16910)
                     /gene="metA"
                     /locus_tag="ACHK74_003958"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFP0222693.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            17067..17510
                     /locus_tag="ACHK74_003959"
     CDS             17067..17510
                     /locus_tag="ACHK74_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP0222694.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            complement(17625..>17729)
                     /gene="rrf"
                     /locus_tag="ACHK74_003960"
     rRNA            complement(17625..>17729)
                     /gene="rrf"
                     /locus_tag="ACHK74_003960"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         4322 a         4639 c         4456 g         4312 t
ORIGIN      
        1 aggaatatta tcagttcagc tcgcaaacga attcccgtac actgaactct ctggctcact
       61 gagggcacca tcatggcact cccccgtatt acccaaaaag agatgactga acgcaagcag
      121 cgtgaactta aaacactgct ggatcgggcg cggattgcgc atggtcgcgt actgaccaac
      181 tccgaaacca acagcattaa gaaagagtac atcgataaat taatggtcga gcgtgaggct
      241 gaagcgaaaa aagcccgcca attgaagaaa aagcaggctt ataaaccgga tcctgaagcg
      301 tcatttagct ggtcagcaaa tacgtcgacg cgcggcaggc gctaatgaac aatcaacgcc
      361 tgagattttc actcgtctgt gtggacacaa tcttcccctc accctaaccc tctccccgaa
      421 ggggcgaggg gactgtccga gctctttttg gacgttttca tcagtctgag gcgttaatca
      481 gcgtcccttc tttttacgcc ccggcgaggt aaaacgctta ccgttggacg ccgggcgacc
      541 gcctttttcc gccgtttttt ccatcttaac gactggacgt ttgatgcccg ctgttttcgg
      601 tttggccttc gctttaggtt ttgcctcgga agaggaattt tcgatgagct taaagaggtc
      661 gattaactca tcatcagtta aatcgcgcca ctcacccagc ggaatgccgc ttaaactgac
      721 gttcataatg cgcgtacgtt ccagcttttt cacttcgtaa ccaaagtgct cgcacatgcg
      781 acggatctga cggttcagcc cctgcaccag ggtaatgcgg aagacaaacg gcgcttcttt
      841 tttgacttta cactttttag tcactgtccc gaggattggc acccccgccc ccataccacg
      901 aataaattca tcggtaatcg gtttatcgac cgtaaccaga tactctttct catgatcgtt
      961 acccgcacgg aggatcttat tcaccagatc gccgtgattg gtgaggaaaa tcagcccctg
     1021 ggagtcttta tccaggcggc caatcgggaa cacgcgtttg ctgtggttaa cgaaatcgac
     1081 aatgttatcg cgctcgccat cttcggtggt gctgacaata ccgacgggct tgttcagggc
     1141 gataagtacc aaatcttcgg cttcccgagg ctcaatcaac tgaccgttta ctttcacaac
     1201 gtcgccgggt ttcacctgat cgccaatggt ggctcgcttg ccattaagga acacattgcc
     1261 ttgctcgata tagcgatccg cttcgcggcg tgagcaaatt ccgctttcgc tgatgtattt
     1321 atttaaacgg actgatgagt cgggcagcat aaattctcct gtaaaaaacg gaatataccg
     1381 tacctttggg ttgataaaaa atagtcatga gcgaactatt tgttatttcc attggcttga
     1441 attcgttacc tgctcaaatc actttccaga agcaatgtgc gacatcgtga gccatgtcag
     1501 taagtggtta aattcgcagt ggtagcatgc cttccgtgat ttcttactaa ccggagaagt
     1561 aatggaactg cttttattga gtaactcgac gctgccgggt aaagcctggc tggaacatgc
     1621 actgccgcta attgctgaac agttgcaggg tcgccgctca gcggtgttta tccctttcgc
     1681 tggcgtaacg cagacctggg atgattacac agcgaaaacg gctgcggttc tcgctccgct
     1741 gggtgtttct gtcaccggta ttcatagcgt tgtcgatccc gttgccgcga ttgaaaatgc
     1801 tgagatcgtg attgtcgggg gcgggaatac tttccagttg ctgaaacagt gccgcgagcg
     1861 cgggctgctg gcaccaatta ctgacgtggt taaacgtggt gcgctgtata ttggctggag
     1921 cgcaggcgct aaccttgctt gcccaaccat tcgtaccacc aacgatatgc cgattgtcga
     1981 tccgcaaggt ttcgatgcgc taaatctgtt cccgctgcaa atcaacccgc acttcaccaa
     2041 cgcgctgccg gaaggccata aaggtgaaac ccgtgagcag cgtattcgcg aactgctggt
     2101 cgtcgcgcca gaactgacga ttattggtct accggaaggt aactggatca cagtgagtaa
     2161 aggtcacgct acgctgggtg gcccgaacac cacttatgtg tttaaggctg gtgaagaagc
     2221 ggtgccgcta gaagctggtc accgttttta agtgaattgc cggatgcatg atccggcatc
     2281 ttgttcatag tgcctgatgc gacgcttgac gcgtcttatc aggcctacag gattcacaat
     2341 attaatactc gtcccgtcct tcatcttcat ccggctgttc cagcacactg taagccaccg
     2401 aacaaaacag cgagttcagg cgctgcatat ctcccagtaa gcctaaatgt agcgaactgg
     2461 tttcaatgct ctgcacgttt tgctgatgca ggcgatcgac gtgggcgtgg gaatagcggc
     2521 gattaagaat gcgaaaacga tgtttgctgc gacgcaaacg acgagcgctg gtgacatcgc
     2581 cagagaagaa caccgacatt gccagtttta aattactgag caattgctca tagagcgcat
     2641 ccagttcctt caagccatca agcgaaaatg cccgccgtgc tgccagcgat ttatcggcaa
     2701 tttcgctgcc catgcgctcg acgatatcgg aggcctgttc aaggttgagc gacatttcga
     2761 tgatctccgc ccagcggcgc gactcttcct ccgccaactc ttcttttggc atccgcgcca
     2821 gatacagctt aatggcggtg tagagaacgt tgatatcatc tgccagctta cgcagctctt
     2881 tctcctgccg tggctcgccg tgcatcactt tatttagccc ttccatcatc tgttccatgg
     2941 cgtcgccaat gcgcagggtt tcgcgcgcgg cgttcgccag agcaagcgtg ggcgtatcca
     3001 gcgcgctgac atccagatgt ttgggccgta gctgggtatc cagttccggt tcatcgcgaa
     3061 taatcgtttt gcaaaaccgt gccatcgggt caacaaatgg cagcatgacc aggcaacgta
     3121 caaggttgta gaagacgtgg aaatagatca ccagttccgc ttttggcaat gacaacttcc
     3181 ccattgtctc tgccagcaaa tggacaaacg gcaggataat caggctaccc accagcttaa
     3241 acagcagact acccagcgcg acacggcggg ctgcggcatt ggcggcactg ttgttgagca
     3301 tcgccagcag accggaaccg aggttagcac caatcaccag acagagcgcc acggggaagg
     3361 agataatgcc tgcggcggtc agagtcgcgg tcagcagtac agcagcaagg ctggagtagc
     3421 tgataatggc gaacatcgcg ccaatcagcg catccagcag aatatcgccg gtcagcgagg
     3481 caaagatcac ctgaacgccg tttgcctggg tgatcggcgt tacggcctgc acaatcaact
     3541 ccagcgccag caaaatcagc ccaagaccaa taccgacgcg gcccagttgc ccggcgcgtg
     3601 actgtttgcg tccgaggaaa aaaatcacgc cgataaaaat aagtaacggt gacagccagg
     3661 ataagtcgaa ggtgagaata cgcgccatta gcgccgtccc gacatctgca cccagcacaa
     3721 tgaccagagc cggtgcgagg gctaccagat cctgagcgac aaacgaggtc accagcatgg
     3781 tggtggcatt actgctctgt accagtgcgg taacgccgat ccccgcgcaa aaggcgagcg
     3841 gctttttttc gacgctccgg ctaaggacag tacgcaaacg cgcgccgaag acgcgcatta
     3901 cgccggttcg aacaatatga gtcccccaga ccagcagggc gacggcagaa agcaggtgaa
     3961 gcagcgttaa cacgagttca ggttctcctt atcgttatat gttcctgaag caattgaatg
     4021 accaaaggac cggaaacacg ctgactggca tctttactgt gccttttgac cttcgttttt
     4081 ccggctaaga ttattccagc ctgattccag tataagtgtt tcatattaat aaagagacag
     4141 ggcactcatt gagcgccctg tttgttgtaa ggaaaagtga cagatttgtg aatcagtccg
     4201 cgtcataccc cagattcggt gccagccagc gctcaacttc ggtaacgctc atacctttac
     4261 ggcgggcata gtcttcaacc tggtcgcgct gaatttgcgc taccgcataa tacttgctgt
     4321 ccgggtggct gaagtaccaa cccgaaaccg atgcaccagg ccacatggca aaagattctg
     4381 tgagtttcat gccagtgtgt ttttccactt ccagcagctc ccagatggtg gctttttccg
     4441 tatgttccgg gcaggccggg tagcccggtg ccggacggat gccctggtag ttttcgcgga
     4501 tcagttcttc gttgctgagg ttctcgttcg gcgcatagcc ccagtagact ttacgcacac
     4561 gctcatggag atactccgca aaggcttccg ccagacggtc ggcaagcgct ttcaccatga
     4621 ttttgttgta atcatcgtgc tgcgcttcaa aggcatcagc cagtgcgtcc tcttccagcc
     4681 cgccagtcac ggcaaatgcg ccgatgtaat ctgctttacc agaaagcttc ggcgcaacga
     4741 agtcagcgag acagtagtta gggaatcccg ttttttcggt ctgttgacgc agatggtggc
     4801 tgacgttgat cacatgggtg cgcgtttcgt cacggtagat ttcaatgtca tcgcccacac
     4861 ggtttgccgg gaacaggccc accacgccac gcggattcag agttttctcg gcgcttaatt
     4921 tatccagcat gtcgttggcg tctttaaaca gccgctgcgc ctcaacgccc accacttcat
     4981 cttccagaat gcgcggatac ttcccggcca gcgaccaggt cataaagaac ggtgtccagt
     5041 cgatgtaatt acgcagcgtt tcgatgctgg cttcaacttc ctgcacgccg agacggtgcg
     5101 ccaccggcgg cgtgtaagcc tgccagtcaa aagcgaaatc gttatcgcgc gccgcttcca
     5161 gcgtgaccgg tggtgtgcgc ggtttcttac gcccgtgctg aatacgtacg gtttcgtact
     5221 ccttgcgggt acgagcgaca aaatcatcac gctgagtatc ggaaagcagc gccgccacca
     5281 caccaacggt gcgcgatgcg ttctgtacat acaccgtcgg gccgctgtag ttctgctcaa
     5341 ttttcaccgc cgtgtgcgct tttgaggtcg tcgcgccgcc aatcaatagc gggatggtga
     5401 agccctgacg ctccatctct ttcgccacgt taaccatctc gtccagcgac ggcgtgataa
     5461 gccccgaaag tccaatcaga tcagcattca cttctttagc ggtacggaga attttttccg
     5521 caggcaccat aacgccgaga tcgacaattt cgtagttgtt acattgcagc accacaccaa
     5581 cgatattttt accgatgtcg tggacgtcgc ccttcacggt ggcgatcacc atcttgccgt
     5641 tggttttgcc ctgctctttg ctggcttcaa taaacggttc gagatacgcc accgcctgtt
     5701 tcatgacgcg cgccgatttg accacctgtg gcaggaacat tttcccttcg ccaaacagat
     5761 cgccgaccac gttcatgccg tccatcaacg ggccttcaat cacttcaatc gggcgcgtag
     5821 cctgctggcg ggcttcttcg gtatcctgct cgataaactc ggtaatgcct ttgaccagcg
     5881 agtattccag acgtttattc acttcccacg agcgccactc cgcctgctgg gcgttagcgg
     5941 tgtcgtcggt tttgctgccg cgatatttct cggcaagctc cagtaaacgc tcagtgccat
     6001 catcgcgacg attaagaatc acatcttcca ccgcgtcgcg cagttcagcg ggcaggtcgt
     6061 cgtaaatcgc cagctgcccg gcattgacga tccccatatc catgccattg cgaatagcgt
     6121 agtagaggaa cactgcgtga atggcttcgc gcaccggatc gttgccacgg aacgagaaag
     6181 aaacgttaga tacgccgccg gaaattagcg cgtgcggcag ttcgcgtttg atgtcttcac
     6241 acgcgccgat aaagtcctgc gcgtagttgt tgtgctcttc aatgccggtg gcaaccgcga
     6301 agatgttcgg gtcgaagatg atatcttctg gcgggaagcc aacctcttcg gtgaggattt
     6361 tgtacgcccg acggcaaatc tcgattttcc gtgcgcgggt atcggcctgt ccctgttcgt
     6421 caaaggccat taccaccact gccgcaccgt agcgacgcaa caatttcgcg tgatggataa
     6481 aggcgtcgac gccctctttc atcgagatag agttaacaat gcctttgccc tggatacact
     6541 tcagaccttt ttcaatgaca tcccattttg aggagtcgat cataatcggc acacgagcga
     6601 tatccggttc accggcaatc agattgagaa aacgcaccat cgccgcttcg gcatcgagca
     6661 tcccttcatc catgttgata tcgataatct gcgcgccgtt ttccacctgc tggcgggcca
     6721 catccagtgc ctcgctgtat ttctcttctt tgatcaggcg cttgaactta gcggaaccgg
     6781 tgacgttggt gcgttcaccc acgttcacaa acaggctatc ttcgccaatg ctcagcggct
     6841 ccaggccgga caaacggcag gctacgggga tttccggcag tttgcgaggc gctaatcctt
     6901 ctactgcacg actcatcgct gcaatatgtt gtggcgtggt gccacagcag ccgccgacga
     6961 tattgagaaa acccgcttgc gcccattcac gtatctgttt tgccatcgtg tcggcgtcga
     7021 gatcgtactc accaaaggcg ttgggtagcc cggcgttcgg gtgcgcggtg acgtagcatt
     7081 ccgcaatccg tgacagctcc tgcacgtact ggcgcagttc atcgggcccc agcgcacagt
     7141 tcaggccaaa ggtcagagct tcggcgtggc gcaatgagtt gtaaaatgct tcggtggtct
     7201 gcccggagag cgtgcgcccg gaggcgtcgg tgatggtgcc ggagatcata atcggcagct
     7261 caacgcccag cgcttcaaac tccgttttca ccgcaaaaac cgccgctttg gcgttaaggg
     7321 tgtcgaaaac ggtttcaatc aggatcagat ccgcaccacc ttccaccagc gctttggtgg
     7381 attcgcgata ggccgccacc agctggtcaa aagtgatgtt gcggaatgcc ggatcgttga
     7441 cgtccggaga aatagacgcc gtgcggttgg ttgggccgag aacaccggca acgtagcgcg
     7501 gtttctccgg cgtgcgcgcg gtccactcgt cagcacaggc gcgcgccagt ttcgccgccg
     7561 caaagttgat ttccgccgac agggattcca tctggtaatc cgccatcgca atggtcgtgg
     7621 agttgaaggt gttggtttcg atgatatccg cgcccgcttc aaagtaggcg ttgtggatag
     7681 cggcgatcac ttccggtttg ctgagcacca gcaggtcgtt gttgcctttg agatcgcatg
     7741 gccagtcggc aaagcgttca ccacgaaaat cggcttcgtt cagtcgatag ctctggatca
     7801 tggtgcccat accgccgtcc agcaccagaa tacgttcatt taactgcgca cgcagttgtt
     7861 ccactttgct gctcacactt gctcccgaca cgctcaaccc agatttaata aaaattcaac
     7921 aaaccatact ggcataaacg catctgtggt aaaagcgacc accacgcaac atgagatttg
     7981 ttcaacatta actcatcgga tcagttcagc aactattgca ttagctaaca ataaaaatga
     8041 aaatgatttc cacgatacag aaaaaggagg ctgtcatggt cgcacccatt cccgcgaaac
     8101 gcggcagaaa acccgccgtt gccaccgcac cagcgactgg acaggttcag tctttaacgc
     8161 gtggcctgaa attactggag tggattgccg aatccaatgg cagtgtggca ctcacagagc
     8221 tggcgcaaca agccgggtta cccaattcca cgacccaccg cctgctaacc acgatgcaac
     8281 agcagggttt cgtgcgtcag gtcggcgaac tgggacattg ggcaatcggc gcacatgcct
     8341 ttatggtcgg cagcagcttt ctccagagcc gtaatttgtt agcgattgtt caccctatcc
     8401 tgcgcaattt aatggaagag tctggcgaaa cggtcaatat ggcggtgctt gatcaaagcg
     8461 atcacgaagc gattattatc gaccaggtac agtgtacgca tctgatgcga atgtccgcgc
     8521 ctatcggcgg taaattgccg atgcacgctt ccggtgcggg taaagccttt ttagcccaac
     8581 tgagcgaaga acaggtgacg aagctgctgc accgcaaagg gttacatgcc tatacccacg
     8641 caacgctggt gtctcctgtg catttaaaag aagatctcgc ccaaacgcgc aaacggggtt
     8701 attcatttga cgatgaggaa catgcactgg ggctacgttg ccttgcagcg tgtattttcg
     8761 atgagcaccg cgaaccgttt gccgcaattt caatttccgg accgatttca cgcattaccg
     8821 atgaccgcgt gaccgagttt ggcgcgatgg tgattaaagc ggcgaaggaa gtgacgctgg
     8881 cgtacggtgg aatgcgctga ctttttctgg cgggcagagg caatattctg cccatcatac
     8941 ctgagtggca atagaataag ggtgtctgtt aatcgcattg acgccaaaat aacttaatgt
     9001 catacacttc acacactttt attaactcag cattattttt aaacatcaaa ccacttaatc
     9061 attaacaaaa tcgcaagatc tcattccacc gaaactttta cggctgcatt atgagattga
     9121 gcgatttatg atatttcttt acttgtaatc atattaaatt ttttcactgt tctgatagtt
     9181 aaaattcaag acatcaataa acaatgagat atttaaatga ttactcgtat ccctcgtagt
     9241 tctttctctg caaatattaa taatacagcc cagacaaatg aacaccaaac cctgagtgaa
     9301 ttgttttata aagaactcga ggataaattt tctggcaagg agctggcgac gcctctatta
     9361 aaaagcttct cagagaactg tagacaaaat ggtcgtcata tctttagcaa caaggatttt
     9421 gtcattaaat tttccacgtc cgtcttacaa gctgataaga aagaaattac gataattaat
     9481 aaaaacgaaa acacgacact tactcaaacc attgccccaa tatttgaaga atacctaatg
     9541 gaaattttac ctcaacgctc agacactctt gataaacaag aattaaacct aaaatcagat
     9601 agaaaagaaa aagaattccc aagaattaaa cttaatggtc aatgttattt tccggggcga
     9661 ccccaaaacc gtatagtatg ccgacacatt gctgcacaat atattaatga tatttatcag
     9721 aatgttgatt acaaacccca tcaagatgat tactcttcag ctgaaaaatt tctcacgcac
     9781 ttcaacaaaa aatgcaaaaa ccagactttg gcgttggttt ccagccgtcc tgaggggcgt
     9841 tgcgttgctg cctgcggtga tttcgggcta gttatgaaag catattttga caagatggaa
     9901 tcaaatggca tcagtgttat ggcagccata ttactggtgg ataaccatgc tttgacggtc
     9961 cggctaagaa taaagaacac aactgaagga tgtacccatt acgtggtttc ggtttatgat
    10021 cctaatgtaa ctaacgataa aataagaatt atgagcgaaa gcaaagagga tattaaacac
    10081 tattctctga tggattttat gaatgtagat tatagcctcc tgaaatggtc aaatgatcat
    10141 gttattaacc aatctgttgc aataattcca gcacttccga aagaacagct attgatgtta
    10201 aaaggatctg tggatgaaat aacccctcca ttatcaccag caacgatgaa tttgctaatg
    10261 gcaattggtc agaatcacca acttacgcaa ctgatgattc agctccagaa aatgccagaa
    10321 ctacatagaa cagaaatgtt gactgcctat aatagtggac atatgaacgt tattaatact
    10381 atttttaacg cattacccac tctgtttaat acgtttaaat tcgataaaaa aaatatgaag
    10441 cccctcctcc tggcaaataa ttctaatgaa tatcccggtt tgttttcagc gatacagcat
    10501 aaacaacaaa atgttgtaga gacggtttat cttgctttat ctgaccatgc acgcctgttt
    10561 ggatttaccg ctgaagatat tatggatttt tggcaacaca aagcgccaca aaaatactct
    10621 gcctttgagt tggcttttga atttggtcac cgggttattg ctgaattaat ccttaataca
    10681 ttaaataaga tggctgaaag ctttggcttt acggataacc ctcgatacat tgcggagaaa
    10741 aattatatgg aagctttact caaaaaagca tctccccata ccgtacgcta aatctttgcc
    10801 tgcgccgata cgcataaaca tcttccacat gcccttcacg tatgcggttt tgtagtgcgc
    10861 gccagtaatc agcgcggaac aggtcggcgt gcatctcttc aaacagcgga ccaatacgcg
    10921 ggtcggcgca tagccagtgg cgaaactctt ccgggaaaac atcgcccggc gagacgctgt
    10981 accacggttc gctggcaagt tcgtcttccg gatagcgcgg cggcgggatg tcgcggaaat
    11041 tcacttccgt catgtagcaa atttcatcgt aatcataaaa aaccacacgc ccgtgacggg
    11101 tgacaccgaa gtttttaaac agcatgtcgc caggaaaaat gttagcagcg gcaagctggc
    11161 gaatagcgtt accgtattct tcaatggcgt cgcgcaactg ctgaccttcc acttgttcca
    11221 gccagatatt gagcggcacc atccgccgct cgatatagag atggcgaatt acaatctgct
    11281 caccaagatc ggtgattttt tccgctgctt cctgaagcag taattccatt aatgccgggg
    11341 aaatatgccg cttctccagc acaaagtttt caaactcctg ggtgtccgcc attcggccca
    11401 cacggtcgtg ctctttcacc agttgatagc aggcacgaac gtgagcggca gacatctctt
    11461 tctgcggcgc gaacttgtct ttgatgactt tgaatacccg atcaaagccc ggcagcgtaa
    11521 acaccaacat caccattcca cgaatacccg gcgcttcaat gaactgctca ttacagccct
    11581 gtagataaac gagatattcg cggtagcttt cggttttggc gtgcttctgg cagccgatag
    11641 ccatatacaa ttcagcagtg gttttacctg gcagaatttc ccgtagccac tcgaccagcg
    11701 ctgcgggcag cggtgcataa accataaaat aagaacgcgc aaagccaaaa acaatgctcg
    11761 cttcggcggt cgtcgtcagg caggtatcaa taaataactc gccgtcgtcc gtctggtgga
    11821 tcggcagtaa gaagggcaac gtgccggaag gcgtgatcag tttgcccacc agccaggcgg
    11881 ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg actttccgcg aggttgtctg
    11941 tccccagcgt ttccgtcaga tggcgaatga tgtaatgaat gtcacggctt ttattctgcc
    12001 agcgcaggcg cagcggcagg tcgctgataa cgcgcatcag tagagattcc cagccgtgat
    12061 cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg aaagcggcgc tctggctgag
    12121 agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg gtcaaataac cgacagtaca
    12181 cggagttaaa aaagctctcc gcaatctcga agcgcgggta atccggcaac agccgcgtgt
    12241 aatgctcttt aacgcgcagt aaaaatgccg cgtccgtact ttggccgtta gtaatgcagc
    12301 gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag atggatacgg tttttcatcg
    12361 cctgctggac agcatgccag tcggcctgtt cgaaacgctg ctgcgccccg gaagtcactt
    12421 cgaggaatcg accatactga gcgtcgaagc cttgcaaaat ggtttgagca atcagtaatt
    12481 ccaggccacg cggcatttag cgccctcatc aggagcagag aattgccgga tgcggcgtaa
    12541 acgccttatc cagcctacgt tcggcaacgg ttgtaggcat gataagacgc gcaagcgtcg
    12601 catcaggcat cgtgcaccga ttgccggatg cggcgtaaat gccttatccg gcctacgttc
    12661 ggcaacggct gtaggcatga taagacgcgc aagcgtcgca tcaggcatcg tgcaccgatt
    12721 gccggatgcg gcgtgaacgc cttatccggc ctacagtcag caacggttgt tgttgcttag
    12781 aactgcgatt cttcagtgga gccggtcagc gcggtgactg aagacgtgcc gccctgaata
    12841 atagtcgtca ctttatcgaa gtaacctgta cccacttcct gctggtgaga tacgaaggta
    12901 tagccatctt tcgcggcggc aaattccggc tgctgcactt tctcaacgta gtgcttcata
    12961 ccctcgccct gggcataggc gtttgccagg tcaaacatgt tgaaccacat gctgtggata
    13021 cctgccaggg tgatgaactg gaacttgtag cccatatccg acagctgctg ctggaagctg
    13081 gcaatagttt tgtcgtcgag gtttttctgc cagttgaacg acggcgagca gttataagcc
    13141 agcagtttgc ccggatattt cgcgtggata gcttgtgcaa agcgacgcgc cagttccaga
    13201 tccggcgtgg aggtttcaca ccagaccagg tcagcatatg gcgcatacgc caggccacgg
    13261 ctgatcgctt gctcaatgcc cgcatgagta cggaagaagc cttcactggt acgctcgccg
    13321 gtaataaatt cgctgtcata cgggtcgcaa tcggaggtga tcagatccgc tgcatcggca
    13381 tcggtacgag caaccagtag ggttggaacg cccgtcacgt cagctgccag acgcgccgcg
    13441 accagtttct gaatagcttc ttgagttggc accaaaactt tgccgcccat gtgaccgcat
    13501 ttcttcactg acgccagctg atcttcgaag tgaactgccg ctgcaccggc ttcaatcatc
    13561 gctttcatca gttcaaaggc attcaggaca ccgccaaaac cggcttccgc atcggcaacg
    13621 atcggcagga agtaatcgac atagcgcgga tcgcccggat caatgcccgc ggaccattgg
    13681 atctgatcgg cacgacggaa ggtgttgttg atccgctcca ccacagctgg caccgagttt
    13741 gccggataga gcgactgatc cggatacatg ctggccgcca ggttagcgtc cgccgctacc
    13801 tgccatcccg acagatagac tgcttcaata cccgctttcg cctgttgcag cgcctgaccg
    13861 ccagtcagtg caccgaggct gttgatgtag ccttttttcg actcgccatg cagcagacgc
    13921 cacattttcg ccgcgcccag ttgcgccagg gtgcattcag gattgactga accgcgtaat
    13981 ttcaccacat cttccgcgct gtatgggcga gtaatgcctt cccaacgcgg ttgagtccac
    14041 tctttctgta attcttcaat ttgttgtgta cgggttttca tgtgcagatg ctccatagtt
    14101 atgtggtggt ttacgctaac aggcggtagc ctggcagggt caggaaatca attaactcat
    14161 cggaagtggt gatctgttcc atcaagcgtg cggcatcgtc aaaacgcccc tgggagaaac
    14221 gttcttcgcc cagttcgctg gcaatgactt tcatctcttc acccagcatc tggcggaaca
    14281 aggctttggt caccggtttg ccattgctca acgttttttg atgatggatc cactgccaga
    14341 ttgaggtacg ggaaatttca gccgtcgccg catcttccat caggccataa atcggcacgc
    14401 agccgttgcc ggagatccat gcttcgatgt actgcactgc cacgcgaatg ttggcgcgca
    14461 taccttcttc ggtgcgttca ccatcacaag gtgccagcag ctgatcggca gtaatcggcg
    14521 cgtcttgttc gcgcatcact tcaagctgat ttttacggga gccgagaatg tcgttgaata
    14581 ccgccattgc catatccgca aggcctgggt gagcaatcca tgtgccatcg tgaccgttat
    14641 tggcttccaa cgctttatcc gcttttactt tgttgagcac ctggttattg cgctcttcat
    14701 ctttgctcgg aataaacgct gccatgccgc ccatcgcaaa agcaccgcgt ttatggcagg
    14761 ttttaatcaa caggcgtgag taagcattca ggaatggttt atccatcgtc actgcctgtc
    14821 tgtctggcag gacgcgatcg ggatagtttt tcaacgtttt gatatagctg aagatgtaat
    14881 cccaacgacc gcagttcaga ccaacaatat ggtcacgcag cgcgtgaagg atttcatcca
    14941 tctggaacac ggcgggcagc gtttcaatca gcaacgtcgc cttgatggtg ccgcgcggca
    15001 gattaaagcg atcttctgca tagctgaaga cttcgctcca ccaggccgct tcctgccagg
    15061 actgggtttt cggcagatag aaatagggac cgctgccctt tgccaacagt gcctgatagt
    15121 tgtggaagaa atagagcgca aaatcaaaca ggctaccggg gattgcctca ccacgccagg
    15181 tgacatgttt ttccggcaag tgcagaccgc gtacccgaca aatcaaaacc gctggattgg
    15241 gcttgagctg ataaattttg cctgcttcat tggtatagct gatggtgccg ttaaccgcat
    15301 cgcgcaggtt aatttgcccg tcgatcactt tgttccagtc tggtgccagt gaatcttcga
    15361 aatcggccat aaagactttc acattggcgt tcagtgcgtt gatcaccatc ttgcgctcaa
    15421 ccgggccagt tatctcgact cgacgatctt gtaagtccgc gggaatacca cgaattttcc
    15481 agtccgtatc gcgaatggaa gctgtttccg aaataaaatc aggcaaagtt ccgttatcaa
    15541 tatcttgctg ctgctgaatg cgcgctgcca gaagtttatt gcgttgtggc gtaaaatgcg
    15601 tcaccagctc agtcagaaat tctaccgctt cggcagtaag aatttgcttc ccctgctcgc
    15661 catacggcct tatgaaagcc agttcatcgg ttgttgttgc ctgttcagtc atcgtgcagc
    15721 tcctcgtcat ggatccgaaa acttccccac tgtgaacgaa ggatcgttgt gcgcttttcg
    15781 ttcagcacaa ctaagactac tcatttaaaa tgcaaaatca aaaacaattt ccattttaat
    15841 taaaaatact tgataactta ttgataacaa tatgaataaa atttattcac ctcctgaggt
    15901 gcgtttcgga aataaaaaag gcacccgaag gtgcctgagg taaggtgctg aatcgcttaa
    15961 cgatcgacta tcacagaaga ttaatccagc gttggattca tgtgccgtag atcgtatggc
    16021 gtgatctgat agacgtaata gttgagccag ttggtaaaca gcaaattacc gtgactacgc
    16081 cagctcgctc gcggtgtatt ttgcggatca ttgtgcggga aatagttata cggtacttcc
    16141 gggcctagtc cggcttccac atcgcggaaa tattcctgcg ccagcgtttg cgcatcatat
    16201 tcgggatggc ccgtcacaaa ggcaatgcgc ttatctttgc tggcaaacag atatgcatcc
    16261 ccttcttccg tctctgccag aatttccaga tcggtgtaat cacgaatcaa cgctgccgga
    16321 aagtcagcat agcgcgaatg tggtgccagg aatgaatcat caaagccacg cgtcagaagc
    16381 gcatgaggat ggagaatatg atgctcgtaa acgccagaga gtttgtcggt gcgagtttgc
    16441 ttaggaatgc cgtagaggat attgagcgcg gcctgtaccg cccagcagac aaacagcgtc
    16501 gaggtgacgt gatctttcga ccactccagc acctgtttga tctgcggcca gtaagcgaca
    16561 tcattaaact ccaccaggcc cagcggcgca ccggttacaa tcagaccgtc aaagttctgt
    16621 tcctgaatat cttcaaagtt acagtagaag ttgttcagat gctctgcggg cgtgttgcgc
    16681 gattcacggg aatcgatgcg caacagctga atatcgacct gcaaaggtga gtttgaaagc
    16741 aggcgcagaa actgattttc agtttcaatc ttcttcggca tcaggttaag gatcagaacc
    16801 ttaagtggac gaatttcctg accagacgca cgagaagttg tcatcacaaa gacgttttct
    16861 tcacgcaaga aattgacggc gggtagctcg tccggcacac gaataggcat aacctgatta
    16921 cctcactaca tacgcttata cgtttagaca tccagatagc tgaagataac cagaaaattt
    16981 gccaatgtcg agcctgcatg ttgaaggtga gaaagtttca aggaaaaggc tacgttagaa
    17041 tataagaatg acgaaaagga gagaaaatgg ttattagtat tcgccgctca cagcatgagg
    17101 aaggggagga actcgttgcg atttggtgtc gatctgtcga tgccacccac gattttttat
    17161 cagcagagta tcgggccgag ctggaagagc tggttcgttc ctttctgccg gaagcaccgt
    17221 tgtgggtcgc ggttaatgaa cgggatcagc cggttgggtt tatgttgtta agtgggcagc
    17281 atatggatgc gctgtttatc gatcctgatg tgcgcggctg cggcgtgggt cggatgctgg
    17341 tggagcatgc gctctcgatg gcaccggaac tgacaaccaa cgttaatgag caaaatgagc
    17401 aggcggttgg gttctataag aaggtgggtt ttaaggttac gggacgctct gaggtggacg
    17461 atttggggaa accgtatccg ttgctgaatc tggcgtatgt gggggaataa gccgcccctg
    17521 ccgtgcctgc ccaggctgga gaagacgcgc cagcgtcgca cccggcattt ttttcaccaa
    17581 cgcaaaaagg ccatcgtgac tgatggcctt tcgcttaatt tgatgcctgg cagttcccta
    17641 ctctcgcatg gggagacccc acactaccat cggcgctacg gcgtttcact tctgagttcg
    17701 gcatggggtc aggtgggacc accgcgcta
//
LOCUS       DAZGSP010000106        17245 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZGSP010000106 DAZGSP010000000
VERSION     DAZGSP010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.107"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            46..630
                     /locus_tag="ACHK74_003961"
     CDS             46..630
                     /locus_tag="ACHK74_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0222695.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            710..1405
                     /locus_tag="ACHK74_003962"
     CDS             710..1405
                     /locus_tag="ACHK74_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0222696.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1435..3951
                     /locus_tag="ACHK74_003963"
     CDS             1435..3951
                     /locus_tag="ACHK74_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0222697.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNRHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3962..5053
                     /locus_tag="ACHK74_003964"
     CDS             3962..5053
                     /locus_tag="ACHK74_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0222698.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GTNTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFRNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(5096..5956)
                     /gene="rsmI"
                     /locus_tag="ACHK74_003965"
     CDS             complement(5096..5956)
                     /gene="rsmI"
                     /locus_tag="ACHK74_003965"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFP0222699.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALVAEIHGVKKNA
                     LYKYALEQQG"
     gene            6020..8056
                     /gene="lpoA"
                     /locus_tag="ACHK74_003966"
     CDS             6020..8056
                     /gene="lpoA"
                     /locus_tag="ACHK74_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFP0222700.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            8014..8409
                     /locus_tag="ACHK74_003967"
     CDS             8014..8409
                     /locus_tag="ACHK74_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFP0222701.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8429..9019
                     /gene="diaA"
                     /locus_tag="ACHK74_003968"
     CDS             8429..9019
                     /gene="diaA"
                     /locus_tag="ACHK74_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFP0222702.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            9029..9604
                     /gene="dolP"
                     /locus_tag="ACHK74_003969"
     CDS             9029..9604
                     /gene="dolP"
                     /locus_tag="ACHK74_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFP0222703.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9718..10758)
                     /locus_tag="ACHK74_003970"
     CDS             complement(9718..10758)
                     /locus_tag="ACHK74_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP0222704.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWEPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGTVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10831..11466)
                     /locus_tag="ACHK74_003971"
     CDS             complement(10831..11466)
                     /locus_tag="ACHK74_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFP0222705.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAESMRPEHEGVTILSSSELRKRAE"
     gene            11594..12112
                     /gene="yhbO"
                     /locus_tag="ACHK74_003972"
     CDS             11594..12112
                     /gene="yhbO"
                     /locus_tag="ACHK74_003972"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFP0222706.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12092..12535)
                     /locus_tag="ACHK74_003973"
     CDS             complement(12092..12535)
                     /locus_tag="ACHK74_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFP0222707.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            12586..12888
                     /gene="yhbQ"
                     /locus_tag="ACHK74_003974"
     CDS             12586..12888
                     /gene="yhbQ"
                     /locus_tag="ACHK74_003974"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFP0222708.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12875..13378)
                     /locus_tag="ACHK74_003975"
     CDS             complement(12875..13378)
                     /locus_tag="ACHK74_003975"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0222709.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13372..13896)
                     /locus_tag="ACHK74_003976"
     CDS             complement(13372..13896)
                     /locus_tag="ACHK74_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFP0222710.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14105..15100
                     /locus_tag="ACHK74_003977"
     CDS             14105..15100
                     /locus_tag="ACHK74_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFP0222711.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15109..15987
                     /locus_tag="ACHK74_003978"
     CDS             15109..15987
                     /locus_tag="ACHK74_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFP0222712.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16193..17200
                     /locus_tag="ACHK74_003979"
     CDS             16193..17200
                     /locus_tag="ACHK74_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFP0222713.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPNVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
BASE COUNT         4349 a         4550 c         4362 g         3984 t
ORIGIN      
        1 cacattaaat atgattatgt acttgttaca aggataaggt tataaatgaa taaagttaca
       61 aaaacagcta ttgtgggctt acttgcgctt ttcgcaggta atgccgctgc aaccgatggt
      121 gaaatagttt ttgatggcga gattttaaaa tccgcttgtg aaatcaatga ctctgataag
      181 aaaattgaag ttgctcttgg tcactataat gccgaacagt ttcgttctgt tggcgatcgt
      241 agcccaaaaa ttccattcac tattccttta gttaactgcc cagtgactgg gtgggagcac
      301 gataacggca acgttgaagc gtctttccgt ttatggctgg aaacgcggga taatggcacc
      361 gtccctaact tccctaatct ggcaaaagtg ggctcttttg ctggcacagc ggcaacaggt
      421 gtgggtatcc gtattgacga tgcggaaagc ggaaacctta tgccactgaa tgctatgggg
      481 aatgataaca cggtttatca gatcccagcg gactccaatg gtattgtcaa tgttgacctc
      541 atcgcttact acgtatcaac cgttgaagca tcagaaatca ccccagggga agcagacgct
      601 gtcgttaacg taacgctgga ttaccgttaa tacgttacgg cgttatctga cctttcagat
      661 aacgcccttt tccttcctct ttctcgttgt atcaggttga aaaataacta tgtcaaaacg
      721 aacattcgcg gtgataataa ccttgttgtg tagcttctgt atcggccagg cgcttgcagg
      781 gggtatcgtt ttacagcgaa cgcgagtgat ctatgatgcc agccgcaaag aggctgcgtt
      841 acctgtcgca aacaaaggcg cagaaacgcc ttatttactg caatcatggg tagataatat
      901 agatggtaca agccgtgccc catttattat aaccccaccg ctatttcgtc ttgaagctgg
      961 cgatgactca tcactgcgaa ttattaaaac agctgataac ctgcctgaaa ataaagagtc
     1021 gctgttctac attaatgttc gtgccattcc agcaaagaaa aaatcagatg atgttaatgc
     1081 taacgagttg acgctggtat ttaaaacacg gatcaaaatg ttttatcgcc ccgcacacct
     1141 gaagggacgg gtaaacgatg cgtggacatc actggaattt aaacgtagtg accattcact
     1201 caatatatat aacccaactg aatattacgt cgtatttgcc ggactggcag tcgataaaac
     1261 cgatctcaca agcaaaattg aatatatcgc gcccggagaa cataaacagt taccacttcc
     1321 tgcatctggc ggaaagaacg tgaaatgggc tgcaatcaat gattatggcg gcagttccgg
     1381 gacagaaact cgtccactgc aataacaaat ataaaaaaca caggtcatca gggaatgcca
     1441 caacgacacc accagggaca taaacgcaca ccgaaacagt tggcgctcat catcaaacgc
     1501 tgtttgccga tggtgctcac tggcagcggc atgctttgca ctaccgctaa cgccgaagag
     1561 tattatttcg accccattat gctggaaact acaaaaagtg gtatgcaaac aactgatctg
     1621 tcacgttttt caaaaaaata cgcacaacta ccaggaactt atcaggttga tatctggctg
     1681 aataaaaaga aggtttcaca gaaaaaaatt acatttaccg ccaatgcaga gcaacttctg
     1741 cagccacagt ttacggtaga acaactacgt gagctgggta ttaaggtgga tgaaatcccg
     1801 gcgctggctg aaaaagatga cgatagcgtg atcaactcgc ttgaacaaat cattcccggt
     1861 acagctgctg aatttgattt caatcatcag cgacttaatt tgagcattcc ccaaattgca
     1921 ctgtaccgtg atgcaagagg ttacgtctcc ccttctcgtt gggacgatgg tataccaacg
     1981 ctgtttacca actactcgtt tacaggttct gataaccgtt accgccaggg caatcgtagc
     2041 caacgacagt acctgaatat gcaaaatggt gctaattttg gcccctggcg attacgtaac
     2101 tattctacgt ggacacgcaa cgatcaggcg tcaagctgga acactatcag tagttattta
     2161 caacgtgata tcaaggcgtt gaagtctcag ttgcttctgg gagaaagcgc caccagcggc
     2221 agtatttttt ccagctacac ctttactggc gtgcaactcg cttccgacga taatatgttg
     2281 ccaaacagcc agcgcggatt tgccccaacg gtacgcggta tcgcaaacag tagtgcaatc
     2341 gtgactatca ggcaaaatgg ttatgtgatc tatcaaagca acgtgccagc gggtgccttt
     2401 gaaattaacg atctctaccc ctcttccaac agcggcgatt tagaagtcac gattgaagaa
     2461 agtgacggta cgcaacgtcg ctttatccag ccttattctt cattacccat gatgcagcga
     2521 cctgggcatc taaaatatag cgcgaccgct ggacgctatc gcgctgatgc aaacagtgat
     2581 agcaaggaac ccgaatttgc tgaagccacg gcaatatatg gtttgaataa tacttttacg
     2641 ctgtatggcg gcctgctcgg ttctgaagat tattatgcgc tggggatcgg tatcggcggc
     2701 acacttggcg cactgggcgc gttgtcgatg gatatcaaca gagctgacac ccaattcgat
     2761 aaccggcact cttttcatgg ctatcaatgg cgtacgcagt acatcaaaga tatcccggaa
     2821 accaacacca atatcgctgt tagctactat cgctatacca acgatggcta ttttagtttt
     2881 gatgaagcca atacccgcaa ttgggactat aacagtcgcc aaaaaagtga aattcaattc
     2941 aacatcagcc agacaatatt tgatggggta agtctgtatg cctccggttc gcagcaagac
     3001 tattggggca ataacgataa aaacaggaat atctcagttg gggtttccgg ccagcaatgg
     3061 ggaattggtt acagcctgaa ttatcaatac agtcgctaca cggatcaaaa taatgaccgc
     3121 gcactctctt tgaatctcag tattccgtta gaacgctggt taccgcgtag ccgggtttcc
     3181 tatcagatga ccagccagaa agatcgccca acccaacatg aaatgcgtct tgatggctca
     3241 ctgctggatg atggtcgcct gagctatagc ctggaacaaa gtctggatga cgataacaac
     3301 cataacagta gcctgaacgc cagttaccgt tcaccttatg gcaccttcag tgccggatac
     3361 agttacggta atgacagcag ccaatacaat tacggcgtta ccggcggcgt ggttatccat
     3421 cctcatggcg tgacgctctc gcaatatctg ggcaacgctt ttgcgcttat cgatgctaat
     3481 ggggcttctg gcgtgaggat acaaaactat ccggggattg ctactgatcc ctttggctat
     3541 gcagtggttc cttatctcac gacttaccag gaaaaccgtc tctcggtaga tactacgcag
     3601 ctgcccgata acgtcgatct tgagcaaaca acacagtttg tggtgcccaa cagaggtgca
     3661 atggtagcgg cgcgtttcaa cgccaatatc ggttatcgcg tacttgttac agtcagcgat
     3721 cgcaacggta aaccgttgcc ctttggcgct cttgccagca acgatgagac ggggcaacaa
     3781 agtatcgtcg atgagggcgg catactatat ctctctggga tatcgagtaa atcacaaagc
     3841 tggactgtac gctggggaaa tcaggcagat caacaatgtc agtttgcttt tagtacaccg
     3901 gattcagaac caacaacctc tgtattacaa ggcacggcgc agtgccatta aggataaaaa
     3961 aatgaaaaga gcgcctctta taacaggact tttgttgata tccacatcct gcgcttatgc
     4021 ctcctcagga gggtgtggag ccgacagcac tagcggtgcg acaaattaca gcagtgtggt
     4081 tgatgatgtt acggtgaacc agacagataa cgtgacagga cgggagttta cctctgcaac
     4141 gctaagtagc actaactggc aatacgcctg ttcctgctct gcgggtaagg cagttaaact
     4201 tgtctatatg gtcagccccg tacttaccac cactggacat cagacaggat attacaaact
     4261 caatgacagc ctggatatta aaaccacatt acaggcaaac gacattccag gactcacaac
     4321 cgaccaggtt gtctctgtta acacccgatt cacacagata aaaagcagca ccgtatattc
     4381 tgctgcaacc caaacgggtg tttgccaggg tgacacgtct cgttatggac ccgttaatat
     4441 tggtacaaat accaccttta ccctgtatgt caccaagcca tttctcggct cgatgaccat
     4501 tccgaaaacg gatattgccg tcattaaagg cgcgtgggtc gatggaatgg gaagcccgtc
     4561 tacaggtgac ttccatgatt tagtcaagtt atcgattcag ggaaatctca ccgccccaca
     4621 gtcgtgcaaa attaatcagg gcgatgttat taaggttaat tttggattca tcaatggtca
     4681 gaagtttacc acccgcaatg ccatgccaga cggttttact ccagtagact ttgatatcac
     4741 ttatgactgt ggtgatactt caaagattaa aaactcgttg caaatgcgca tcgacggtac
     4801 aactggggta gtagaccagt acaacctggt cgccaggcga agaagttcag acaatgtgcc
     4861 cgatgtcggt attcgtattg aaaatctcgg cggcggagtt gcaaatattc cttttcggaa
     4921 cggtatcctt cccgttgatc cttccgggca tggcaccgtc aatatgcgcg cctggccagt
     4981 taatctggtc ggtggtgagc tggaaacagg aaaatttcag ggcacagcca ccattaccgt
     5041 catggtgcgg taaaagaaga cgttgcgttt tgattaacgc aacgcttcgg cactgttacc
     5101 cctgctgctc cagcgcatac ttatacagcg catttttctt cacgccatga atttctgcga
     5161 ccagcgccgc cgcttttttc agcggcagtt ctgcctgtag cagcgccagc gtgcgcaggg
     5221 catcggcggg taagtcttct tcctgtgctt tatgaccttc gacaatcagc accatttcgc
     5281 ctttgcgacg gttttcatct tcctttaccc acgccagcag ttcgccaacg ggcgcgccgt
     5341 gaatggtttc ccaggttttg gtcagctcac gcgccagaac cacgtagcgg gattcgccta
     5401 ataccgcaac gatatcttcc aggctatcta acagacggtg ggtggattca taaaaaatca
     5461 gcgtgcgcgg ctccgcttca atggctttta gcgcatcacg gcggcctttt gatttggcag
     5521 gtaaaaagcc ttcgtaacag aaacggtcag agggtaaacc cgcggcgctt aacgcagtga
     5581 tagcagcaca cggcccgggt agcggcacca cacgaatccc cgcttcacgg caggtacgca
     5641 ccagatggta gccaggatcg ttaattagcg gcgttccggc atcggaaacc agcgcaatgt
     5701 tttgcccctc ttgcagcttc gccagcagcg tttcggcttt ttgttgttcg ttatggtcgt
     5761 gcagcgcaaa caaccgggca ttaatcccaa aatgttgcag caataaaccg gtgtgacgag
     5821 tatcctcggc ggcaatcaga tcaacggcct gtaatacctc taacgctcgc tgggtgatat
     5881 ccgccagatt gccgattggc gtcggtacaa tgtaaagctg gccctgagaa ttatccgccg
     5941 attggtgttg tttcattgtg tcgtccgtat tgccgattta atattgagca ttgcgtaaaa
     6001 aaatatcact ggatacatta tggtaccctc aacattttct cgtttgaaag ccgcgcgttg
     6061 tctgcctgtt gttctggcag ccctgatttt cgccggttgt ggcacccata ctcccgatca
     6121 gtccactgct tatatgcagg gcacggcgca ggctgattct gccttttatc ttcagcagat
     6181 gcagcaaagc tctgatgata ccaggatcaa ctggcaatta ctcgccattc gtgcactggt
     6241 gaaagaaggt aaaaccgggc aggcggttga gttgtttaac caactaccgc aagaactgaa
     6301 cgattctcag cgtcgcgaga aaacactgct ggcggtagag attaaactgg cgcagaaaga
     6361 ttttgctggc gcgcaaaact tgctggcgaa aatcacacct gccgatttag aacaaaacca
     6421 gcaagcgcgt tactggcagg caaaaatcga tgccagccag gggcgtcctt ccattgattt
     6481 actgcgcgcg ttaattgctc aggaaccgct gctcggcgcg aaagaaaaac agcagaatat
     6541 tgatgccacc tggcaggcgc tctcctccat gactcaggaa caggcgaata cgctggtgat
     6601 caacgccgac gaaaatattc tgcaaggctg gctggatctg cagcgcgtct ggtttgataa
     6661 ccgtaacgat cccgacatga tgaaagccgg gatcgccgac tggcaaaaac gttatccgaa
     6721 caatccgggc gcgaaaatgc tgccaacgca gttggttaac gtaaaagcgt ttaaaccagc
     6781 ctcgaccaac aaaatcgccc tgctgttgcc gctgaatggc caggcagcgg tatttggtcg
     6841 cactattcag caaggctttg aagcggcgaa aaatatcggc actcagccag tggcggctca
     6901 ggtagctgcc gcacctgccg cagacgtagc tgaacaacct cagccgcaaa ccgtggatgg
     6961 cgttgccagc ccggcacaag cctcggttag cgatctgacc ggtgaacagc ctgcggcaca
     7021 gccagcgcct gtaagcaccc cggcagcaac caccacagcg gtaagcgcgc cagcgaatcc
     7081 atccgcagaa ctgaaaatct acgatacctc atcacaacca cttagccaga tcttaagcca
     7141 ggttcagcag gatggcgcga gtattgtggt tggtccgttg ctgaaaaata acgttgaaga
     7201 gttgcttaag agcaacaccc cgctgaacgt gctggcactg aaccagccgg agaatatcga
     7261 aaaccgcgtc aatatttgtt acttcgcgct ttcaccggaa gacgaagcgc gcgatgcagc
     7321 gcgtcatatt cgtgaccagg gtaaacaagc gccgctggtg ctgatcccac gcagttcatt
     7381 gggcgatcgc gtagccaatg cgtttgcgca ggagtggcag aaactgggcg gcggcaccgt
     7441 tctgcaacaa aaatttggtt ccaccagcga attacgcgcg ggtgttaacg gcggttctgg
     7501 tatcgcttta acgggtaccc cgattactcc cagagcgaca accgactccg gcatgacgac
     7561 caacaatcca acgctgcaaa ccacgccaac cgatgaccag ttcaccaata atggcggtcg
     7621 tgtcgatgcg gtgtacattg tggcaacgcc gggtgaaatc gcttttatca aaccgatgat
     7681 cgccatgcgt aacggtagcc agagcggtgc aacgctgtac gccagctccc gcagtgcgca
     7741 agggaccgct ggcccggatt tccgactgga gatggaaggc ttgcagtaca gcgaaatccc
     7801 gatgctggca ggcggtaatc tgccgttaat gcagcaggcg ctcagcgcgg tgaacaacga
     7861 ttattcgctg gctcgcatgt atgcgatggg cgttgatgcc tggtcgttgg caaatcattt
     7921 ctctcaaatg cgccaggttc agggttttga aatcaacggt aataccggaa gcctgacggc
     7981 aaacccggat tgcgtgatta acaggaagtt atcatggcta cagtaccaac aaggtcaggt
     8041 agtccccgcc agttaaccac caaacagacc ggcgatgcgt gggaagcaca agcgcgtcgc
     8101 tggctggaag gcaaaggact gcggtttatc gccgctaacg tgaacgagcg tggcggcgag
     8161 atcgatctga taatgcgtga aggccggacc accgtttttg tcgaggtacg ctatcgccgc
     8221 tctgcgcttt atggcggcgc ggcagccagt gtgacccgca gcaaacaaca caaattatta
     8281 cagactgccc gcttgtggct cgcgcgtcat aatgggagtt ttgatactgt ggattgccgg
     8341 ttcgatgtgg tagccttcac cgggaatgag gttgagtgga ttaaggatgc ctttaatgac
     8401 cactcataat taaggtttaa ggattagcgt gcaagaaaga attaaagctt gcttcactga
     8461 aagcattcaa actcaaattg cggcggcaga ggcgcttccg gatgccatct cccgtgcagc
     8521 catgacgctg gttcagtctc tgctcaatgg caacaaaatc ctctgttgtg gtaatggaac
     8581 ttccgctgcc aatgcacagc attttgctgc cagcatgatc aaccgtttcg aaacggagcg
     8641 gcccagctta cctgccattg cactaaatac tgataatgtt gtcttaacgg cgattgccaa
     8701 cgatcgctta catgatgaag tgtatgcaaa acaggtgcgg gcgctgggtc atgcgggaga
     8761 tgtattgtta gccatttcca cgcgtggcaa cagccgcgat attgtaaaag cggtagaagc
     8821 tgccgttacg cgtgatatga ccattgtggc attaaccggc tatgacggcg gtgaacttgc
     8881 aggtttgtta gggccacagg atgtggaaat ccgcattcct tcgcatcgta gtgctcgcat
     8941 tcaggaaatg catatgctga cggtaaattg cctgtgcgat ctgatcgata acacgctttt
     9001 ccctcaccag gatgattaag gagaatacat gaaggcatta tcgccaatcg cagtccttat
     9061 ttccgcgctg ctgttgcaag gttgtgttgc cgctgccgta gtgggtaccg ctgctgtggg
     9121 taccaaagcc gcaactgacc cacgcagtgt cggcacccag gtggacgatg gtaccctgga
     9181 agtgcgcgtg aacagcgcat tgtcgaaaga cgaacagatt aagaaagaag cgcgcattaa
     9241 tgtaacggcc tatcagggca aagtgctgct ggttgggcag tcaccaaacg ctgaactttc
     9301 ggctcgcgcc aaacagattg ctatgggcgt agacggtgcc aacgaagtgt ataacgagat
     9361 tcgtcagggc caaccgattg gtctgggcga agcgtctaac gatacgtgga tcaccaccaa
     9421 agtgcgttcg cagctcttaa ccagcgacct ggtgaaatcg tccaacgtga aagtgaccac
     9481 cgaaaacggt gaagtgttcc tgatggggct ggtgactgaa cgtgaagcga aagcggcggc
     9541 agatattgcc agccgggtaa gcggcgtgaa acgggtaact acggcgttta cgtttattaa
     9601 atagcagccc cttgtaatgc ctgatgcgac gcttgccgcg tcttatcagg cctacaacga
     9661 tacaaaccgt aggtcagata aggcgtttac gccgcatccg acatgttttc cctcaaatca
     9721 gaacaacagc gccagcccgc cgacaatcac accagacact gccaccatcg cccccgtcag
     9781 ccataaggct ttcgccggga acgctttgcg cagcataatc agtgacggca aactcaccgc
     9841 cgggagcgtc atcaacaatg ccagcgccgg agcagttccc atacctgcca gcatcatcgt
     9901 ttgtacaatc ggaatttctg ctgccgtggg aatgacaaac aagcatcctg ccaccgccat
     9961 cgccaccacc cacatcaggc tgttatcgac agtaccatcg gcatggggga ataaccagac
    10021 gcgagcggca cccaacacca gtactgcaag gatgtaaacc gggatcgtac tccagaaaag
    10081 cgtccatagc gccctgcccc agcggctaaa aaaaccgccc tgtgcttccg gtatgtcaat
    10141 ttcgaccggt gcctgcgttt gcggtgtttc acgcacccat ttttgcacca gcgtcgcaat
    10201 cagcaacacc atcaccagcc cggccaccag acgaatcgcc gcaaaacccc agccgaggac
    10261 aaagcccata aacaccagcg tcgccgggtt taacaccgga ttgcccatcc agaatgccag
    10321 cgcaccgccc atcgacacct gttggcgacg cattcccgcc gcgaccggag ccgcacagca
    10381 ggtacacatc atgcccggca acgaaaacag cgttcccagc agcgtgccgc gaaagcgcga
    10441 ttgcccaagc gtacgcaaca accagtcacg cgggatcagc acctgaatca acgaaccgag
    10501 gatcaccccc agcaccgccg ctttccatac cgcgaggaaa tagatcatcg cgtaatccaa
    10561 cgccgcctgc catgggttag catccgcctg cgcaaggata gatttaccga tactgtgggt
    10621 ttcggcagca gtaaacgctt tgccgtagta aggttcccat ttcacatacc agagaccggc
    10681 aatgacaacg agaaagaaaa gcgcgggttt ccaccactga atgggcgttg ccgcctgaga
    10741 tgaagactga ccagtcatag cattccccag gaataagtta tgataattga gcgcgtgaat
    10801 attacgctca ctatcaattc ttgggaataa ttattcagct cttttgcgta attctgaaga
    10861 gcttaaaatc gtcacgcctt catgctcagg ccgcatcgac tctgccagca tgacccgcgc
    10921 cacgtctctc gcatcaatgg atttccagtt acctggtaac aaacggaaca gcggcgcaaa
    10981 aagcgtttcg ttcatccgct gtttgctacg atcgcccagt aacatcgacg ggcgagcaat
    11041 ggtcaatttc ggccagttct gggcaattaa tgcttcttcc atctcccctt tgacgcggtt
    11101 atagaaaaac ggcgagtggg cattggcacc catagcactg accaccagca tatgctgcgc
    11161 gcccagccgc cgcccggtta atgcggtatc cactaccagc gtgtaatcgg catgaataaa
    11221 cgcctctttg ctccccgctt ctcgccgcgt ggtgccgaga caacaaaaca caatgtcgat
    11281 gggatcggtg acctgcgcca gcgcgtcagt cagttgcgga tcatggggat tgaacactcc
    11341 tggcatatct cccaacggac gtcgcgtcgg tgcagcaata gcgttaactt tcggttcgtt
    11401 aatcaacatc cgcagcaggt gaccgcccac cagccccgtt gcgcctgtaa tcagtacctg
    11461 actcatcttc gctcctttac agaattgtct gccttgcgct ccccggctca tgcaccaggc
    11521 ttaatagacc atgaggtaat tatccccgat tgtgagaaat tcgcctcatc caatgcaaca
    11581 acgcggagga agcatgagta agaaaattgc cgttttaatc actgatgaat ttgaggattc
    11641 agaatttact tcacccgcag acgagttccg taaagccgga cacgaagtga ttaccattga
    11701 aaaacaagcg ggtaaaacgg tgaaaggcaa aaaaggagaa gccagcgtga ccatcgataa
    11761 atccatcgat gaagtgacgc cagcggagtt tgatgccctg ttgctaccgg gcggtcattc
    11821 accggattat ctgcgtggcg acaaccgttt tgtcaccttt acccgtgatt ttgtgaatag
    11881 cggcaaacct gttttcgcca tctgtcacgg tccgcagttg ctgatcagcg ccgatgtgat
    11941 tcgggggcgc aaactgaccg cagttaaacc gatcattatt gatgttaaaa atgcgggcgc
    12001 ggaattttac gatcaggaag tcgtggttga taaagatcag ctggttacca gccggacacc
    12061 ggacgatctg ccagcgttta accgcgaggc gttacgcctg ctcggtgcct gagtcgcgta
    12121 gccaaatcat ttttttacca aagcccagcg tgttgtcggt gaatttgatt tcatcgagcc
    12181 ggatttccca taccggggcc gacagcattc tggcaaccgg aaagcgacga ttgtacgcct
    12241 tgcgcgcgag gtcgctttcc tcaccttcca gcctgcggat ctcaccttta aactgcacac
    12301 cgcgaattaa cgctaccgtt ttcggctgac cgtttaccgt tccggcaact gccgcctgcg
    12361 gcccgctcat ctgcgcgtgg cgcgtttttt cttccgtcaa aatgtagaag gcgactttct
    12421 gcgcatcaaa aagataaaag gcattagcgc accagagttc cccttcctgc tgcacacacc
    12481 aggtgacaac atgttgtttt gccagccaac ggctgatggc gatgagtgtt tccattgctg
    12541 ttctctttta tactgtgggc ctgaccttaa catgcgatgc agacgatgac accctggttt
    12601 ctttacttga tccgtaccgc cgacaataag ctttataccg ggatcaccac ggatgtcgaa
    12661 cgccgctatc agcagcacca aagcggcaaa ggggcgaaag cactgcgcgg gaaaggagaa
    12721 ctgacgctgg cgttttctgc tccagtgggc gatcgttcgc tggcgttacg ggcggaatat
    12781 cgcgttaagc aactgaccaa acggcaaaaa gagcgtctgg tagcggaagg cgcagggttt
    12841 gcagagctgt taagcagtct gcaaaccccg gagattaaaa gcgattgaaa tgctcgtgat
    12901 actcaaccag gccggtaacg ccattcagcg cgtcatccgc taaacgatgt acctggaagg
    12961 cgctttcagt gcccggccag cggcaacgca gatcgtgatg cgccgccagt tcaaagccga
    13021 aacgactgta cagcgccgga tcgcccagcg tcaccactgc ggcatagccg aactcattaa
    13081 gcgaatcgag tccttcatag accagttggc gtgccagccc ctgtccacgg tatttttcat
    13141 caaccgccaa cggtgccatg ccgacccatt gcaggtcttc gccctgcaca tcaaccggac
    13201 taaatgccac atagccaatg acctgacctt cgtcatctgt cgccaccagc cccagcgtca
    13261 gaaagccatc ttcacgcaga tcgtgaacca gcttcgcttc cgcatcactt tcgaatgagc
    13321 gacgcagcag ggcatcaata cccggcgcat caatgggaat ttctactcga attagcatgg
    13381 ttcacctacc gatgtctgtt tggtttcagg cgcggttttc attcccgcct caacaaaatc
    13441 cgccagttgc agcagcatca tgcgtaaggc tttcggcatt tgctccagtt caatggcgtc
    13501 catcaggttt ttcacataca gccccagctc cgtatcgcct tcaatcacca gccgacgctg
    13561 gaagaagagc gtatccggat cttgtttacg cgccgcaatc atcagcagat cgctggcgtc
    13621 ggcactaaaa ctcacatcag cttgcgcgtt ctggctgacc accagtttgc cattcaccac
    13681 cgaggtaaac cattgcaggt caatatcacg cacatgaata cttaaccagc ggccttcaag
    13741 aaactccagc tcgccatcat ccagcgcctg acggaattgc cagcttaaga cctgctcaag
    13801 aacttggcgt tttagcgcaa atggcgtcag ttttaccggt acactcaaca gagatggccc
    13861 caaatgcaca atacgggaac gcagtttatc caacacgagt tttactccct gtttcaacaa
    13921 tcatcctatt ttgccatatc agaaaaataa catagcggta taaatcaaca attccatatg
    13981 aaattgctgc taccaccaat acaactttaa ctgccttaaa tcaaaaattg tcgcagcaag
    14041 gttaactaaa atcccagttc gttaacattt ttgcgttttg atagcgcaac cttcaggaaa
    14101 aattatggag ctgctctgcc ctgccggaaa tctcccggcg cttaaggcgg ccatcgaaaa
    14161 cggcgcagat gctgtttata tcgggctaaa agatgatact aatgcccgtc acttcgccgg
    14221 ccttaacttt accgagaaaa aattgcagga agcggtgagt tttgtccatc aacatcgccg
    14281 caaacttcac atcgcgatta acacttttgc gcatccggac ggttacgccc gttggcagcg
    14341 cgccgtggat atggcggcgc agctgggtgc cgacgcgctg atcctcgccg acctcgccat
    14401 gctggagtac gccgccgagc gttacccgca tattgagcgt catgtgtcag tgcaggcttc
    14461 ggcgaccaat gaagaggcga ttaactttta tcatcgccat tttgacgttg ctcgcgtggt
    14521 gctgccgcgc gtgttgtcga ttcatcaggt gaaacagctg gcacgggtta ctcctgtccc
    14581 gctggaagtg tttgctttcg gcagcctgtg cattatgtcg gaaggtcgtt gctatctgtc
    14641 gtcgtatctg acgggtgagt cgcccaacac cgtgggagcg tgttctccgg cccgtttcgt
    14701 gcgctggcaa caaacgccgc aggggctgga atcccgcctg aacgaagtgc tgatcgaccg
    14761 ttatcaggac ggcgaaaacg caggttatcc gacgctgtgt aaagggcgtt atctggtgga
    14821 cggcgagcgc tatcacgcgc tggaagaacc aaccagtctc aataccctgg aactgctgcc
    14881 ggagttaatg gctgcgaata ttgcttcggt gaaaattgaa ggccgccagc gtagcccggc
    14941 gtatgtcagc caggtggcga aagtctggcg tcaggctatc gaccgttgta aggccgatcc
    15001 gcaaaacttt gtaccgcaaa gcgcgtggat ggagacgctc gggtcgatgt ccgaaggcac
    15061 gcaaaccact cttggcgcat atcaccgtaa atggcagtga gaaaagcaat gaaatattcc
    15121 ttagggccag tgctgtggta ctggccaaaa gagacgctgg aagaatttta tcagcaggcc
    15181 gccaccagca gcgccgacgt gatttatctt ggtgaagcgg tatgcagcaa gcgtcgggca
    15241 accaaagttg gcgactggct ggagatggca aaatcgctcg ccgggagtgg taagcagatt
    15301 gtgctctcca cgctggcgct ggtgcaggca tcatctgaac tgggcgaact gaaacgctat
    15361 gttgagaacg gtgagtttct gattgaagcc agcgatctcg gcgtggtgaa tatgtgcgct
    15421 gaacgcaaac tgccgttcgt cgccgggcac gcgctgaact gctacaacgc ggtgacactg
    15481 aaaatattgc tcaaacaggg catgatgcgc tggtgtatgc cagtggagct ttcccgcgac
    15541 tggctggtga atctgcttaa tcagtgcgat gagctgggca ttcgtaacca gtttgaagtg
    15601 gaagtcctga gctacggtca tctgccgctg gcctactccg cccgctgctt taccgcgcgt
    15661 tcggaagacc gcccgaaaga tgagtgtgaa acctgctgca ttaagtatcc gaacgggcgc
    15721 aacgtgctgt cgcaggaaaa ccaacaagtg tttgtactca atggcattca gaccatgagc
    15781 ggctacgttt acaacctcgg taacgagctg gcatccatgc agggcctggt tgatgtggtc
    15841 cgcctgtcac cgcagggtac tgacactttc gcgatgctcg acgccttccg cgctaatgaa
    15901 aatggcgctg caccactgcc gttgacggcg aacagcgact gtaacggcta ctggcggcgg
    15961 ctggccgggc tggagctgca agcctaagta aatatgctcg tcatacttca agttgcatgt
    16021 gctgcatctg cattcgttca ccccagtcac ttacttatgt aagctcctgg ggattcactc
    16081 tcttgtcgcc ttcctgcaac tcgaattatt tagagcacag ctcactttgt taacaacttt
    16141 aactactctt taatgcagta ttaaagatta atcggtaaca aagtgagctg ttatgactga
    16201 taaaaccatt gcgttttcgc tactcgatct ggcccccatt cccgaaggtt cttcagcgcg
    16261 agaagcattc tcccactctc tcgatctcgc ccgtctggct gaaaagcgcg gctatcatcg
    16321 ctactggctg gcggaacacc acaatatgac cggcattgcc agtgctgcaa catcggtatt
    16381 gattggctat ctggcggcga ataccaccac gctgcatctg gggtctggcg gcgtgatgtt
    16441 gcctaaccac tcaccgttgg tcattgccga acagttcggc acgctcaata cgctctatcc
    16501 ggggcgaatc gatttggggc tgggtcgtgc tccgggtagc gatcagcgaa ccatgatggc
    16561 gctacgtcgt catatgagcg gcgatattga taatttcccc cgcgatgtcg cggagctggt
    16621 ggactggttt gacgcccgcg atcccaatcc gaatgtgcgc ccggtaccag gctatggcga
    16681 aaaaatcccc gtgtggttgt taggctccag cctttacagc gcgcaactgg cggcgcagct
    16741 tggtctgccg tttgcgtttg cctcacactt cgcgccggat atgttgttcc aggcgctgca
    16801 tctttatcgc agcaacttca aaccgtcggc tcgactggaa aaaccatacg cgatggtgtg
    16861 catcaatatt atcgccgccg atagcaaccg cgacgctgag tttctgttta cctcaatgca
    16921 gcaagccttt gtgaagttgc gccgtggcga aaccgggcaa ctgccgccac cgattcaaaa
    16981 tatggatcag ttctggtcgc cgtccgaaca gtatggtgtg cagcaggcgc tgagtatgtc
    17041 gttggtaggt gataaagcga aagtgcgtca tggcttgcag tcgatcctgc gcgaaaccga
    17101 cgccgatgag attatggtca acgggcagat tttcgaccac caggcgcggc tgcattcgtt
    17161 tgagctggcg atggatgtta aggaagagtt gttgggatag tgtgtcttaa cgcgggaagc
    17221 cttatccgag ctggcaacgc tgtcc
//
LOCUS       DAZGSP010000107        17146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZGSP010000107 DAZGSP010000000
VERSION     DAZGSP010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.108"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..135)
                     /locus_tag="ACHK74_003980"
     CDS             complement(<1..135)
                     /locus_tag="ACHK74_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5445 family protein"
                     /protein_id="HFP0222714.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     K"
     gene            complement(211..828)
                     /gene="yncG"
                     /locus_tag="ACHK74_003981"
     CDS             complement(211..828)
                     /gene="yncG"
                     /locus_tag="ACHK74_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFP0222715.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIAFMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAQNISAIADAVCQLPKLQEVLKRNEII"
     gene            1094..2593
                     /gene="ansP"
                     /locus_tag="ACHK74_003982"
     CDS             1094..2593
                     /gene="ansP"
                     /locus_tag="ACHK74_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFP0222716.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2708..3769)
                     /gene="yncE"
                     /locus_tag="ACHK74_003983"
     CDS             complement(2708..3769)
                     /gene="yncE"
                     /locus_tag="ACHK74_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFP0222717.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            4011..6113
                     /gene="pqqU"
                     /locus_tag="ACHK74_003984"
     CDS             4011..6113
                     /gene="pqqU"
                     /locus_tag="ACHK74_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFP0222718.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(6149..6814)
                     /gene="mcbR"
                     /locus_tag="ACHK74_003985"
     CDS             complement(6149..6814)
                     /gene="mcbR"
                     /locus_tag="ACHK74_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFP0222719.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(7012..8049)
                     /gene="curA"
                     /locus_tag="ACHK74_003986"
     CDS             complement(7012..8049)
                     /gene="curA"
                     /locus_tag="ACHK74_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFP0222720.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8230..8748
                     /gene="mddA"
                     /locus_tag="ACHK74_003987"
     CDS             8230..8748
                     /gene="mddA"
                     /locus_tag="ACHK74_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFP0222721.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDSGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8745..9194
                     /gene="ydcZ"
                     /locus_tag="ACHK74_003988"
     CDS             8745..9194
                     /gene="ydcZ"
                     /locus_tag="ACHK74_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP0222722.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(9195..9428)
                     /gene="ydcY"
                     /locus_tag="ACHK74_003989"
     CDS             complement(9195..9428)
                     /gene="ydcY"
                     /locus_tag="ACHK74_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFP0222723.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9514..9687)
                     /gene="ortT"
                     /locus_tag="ACHK74_003990"
     CDS             complement(9514..9687)
                     /gene="ortT"
                     /locus_tag="ACHK74_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFP0222724.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9882..9977
                     /gene="yncL"
                     /locus_tag="ACHK74_003991"
     CDS             9882..9977
                     /gene="yncL"
                     /locus_tag="ACHK74_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFP0222725.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(10379..11188)
                     /locus_tag="ACHK74_003992"
     CDS             complement(10379..11188)
                     /locus_tag="ACHK74_003992"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFP0222726.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPGNPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISDGNIQERDYKKFNLSEVFRGL"
     gene            complement(11398..12822)
                     /gene="patD"
                     /locus_tag="ACHK74_003993"
     CDS             complement(11398..12822)
                     /gene="patD"
                     /locus_tag="ACHK74_003993"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFP0222727.1"
                     /translation="MQHKLLINGELVTGEGEKQPVYNPATGDVLLEIAEASVEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AVVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(12844..13638)
                     /gene="ydcV"
                     /locus_tag="ACHK74_003994"
     CDS             complement(12844..13638)
                     /gene="ydcV"
                     /locus_tag="ACHK74_003994"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0222728.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDSVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(13628..14569)
                     /gene="ydcU"
                     /locus_tag="ACHK74_003995"
     CDS             complement(13628..14569)
                     /gene="ydcU"
                     /locus_tag="ACHK74_003995"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0222729.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIILIALYLAFVKRLGAFDAL"
     gene            complement(14570..15583)
                     /gene="ydcT"
                     /locus_tag="ACHK74_003996"
     CDS             complement(14570..15583)
                     /gene="ydcT"
                     /locus_tag="ACHK74_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0222730.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDPLMAENLCGMTGSFALRPEHIRLNIPGEMQANGT
                     IQAVQYQGAATRFELTLSGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(15601..16746)
                     /gene="ydcS"
                     /locus_tag="ACHK74_003997"
     CDS             complement(15601..16746)
                     /gene="ydcS"
                     /locus_tag="ACHK74_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFP0222731.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(16991..>17146)
                     /locus_tag="ACHK74_003998"
     CDS             complement(16991..>17146)
                     /locus_tag="ACHK74_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP0222732.1"
                     /translation="ALTHHISIAPGKMFSTGENWSRFFRFNTAWQWGEREEQAVKQLG
                     KLIQERL"
BASE COUNT         4303 a         4376 c         4027 g         4440 t
ORIGIN      
        1 ctttttgaga ttatagaata cctgtgataa caagtgaaac atctgcacaa tgtgaatagt
       61 tagtttcttt tcagaggaaa tgaaatagac aagctgaatg aaggggagag tgatataaat
      121 caaaaaacat aacatgaatt taattaatta cacccatcat tgtggataaa gttactgaat
      181 aataaataac ataatcttct ggtatcccac tcaaataatt tcattccttt ttaatacttc
      241 ttgtaatttc ggtaactgac atacagcatc agcgatcgcg ctgatattct gcgcattatc
      301 ctgaaaccat tcatgccccg gcccccatgt gcgcatggtg cagagataac agtccaccag
      361 cgttagttgc tcaccaaagg cataaggttc agcggtcaat tgtgaattca gccagatata
      421 aagtgatttg cgatattcaa ttacattctt ctttaactgt tcaggtgcgt cagatgccca
      481 acgctcaggg taatcggcga aagtgaatgt tggatagaca ttagcaacca gccagaccaa
      541 tagccgttga aactgctgac gttcggcgcg cccaaccggt ggcgcaaggt ccgggcgacg
      601 atcgagaacc ataaatgcaa tcgccgctgt ctccgtcata atctcgtcat tttccagcgc
      661 cagtgtcgga acctgacaca acgggttcag ggttttcaat aactcgcgtg aagctccctc
      721 gtggtcaaaa ccactgacat caacgaactg ataaggaata tcagccagtg ttagcatcag
      781 ctcactgatt gtagagcccc atccgggtac gccatacacc ttaatcatat gccctcacaa
      841 tcttttaaag attaagtgta ggacagtaca caggttaacc agcaaaaaga tttgtcattt
      901 tttcgtcagt tgtattattc cccttcagga ttgtaagggg atgttttgca tcgtttatcg
      961 ataacgggta ggatgctgcg ccgatagttt tatacccata aagaataatg gtgataacta
     1021 tcatcgccag gatgaataaa cattgttcat ggcaacttat atgacttttt cattaaagca
     1081 atcagggaga gcaatgagta aacacgacac cgacacttca gatcaacacg ccgcgaaacg
     1141 ccgctggctt aatgcccacg aagaggggta tcacaaagcg atgggcaatc gccaggtaca
     1201 gatgatcgcc attggcggcg cgattggcac cggcttgttt ttaggtgcag gagcccgact
     1261 gcaaatggcg ggtccagcat tggcactggt ttatttaatt tgtggcttgt tttcgttttt
     1321 tattctgcgt gcattgggtg agctggtgct acaccgccct tccagtggca gttttgtctc
     1381 ttatgcccgt gagtttttgg gtgagaaagc cgcttatgtt gctggctgga tgtacttcat
     1441 caactgggcg atgacgggga ttgttgatat taccgccgtc gctctgtata tgcattactg
     1501 gggtgcgttt ggcggcgtgc cgcagtgggt ctttgcgctc gctgcactta ccatcgttgg
     1561 caccatgaat atgatcggtg tgaaatggtt tgcggagatg gagttctggt ttgcgcttat
     1621 taaagtgctc gccattgtga cctttttggt cgtgggtaca gtgttcctcg gtagtggtca
     1681 gccgctggat ggcaacacca ctggctttca tttaattacc gataatggcg gcttcttccc
     1741 ccacggtttg ctgcctgctc tggtgttgat tcagggcgta gtgtttgctt ttgcctccat
     1801 tgaaatggtg ggtacagctg ccggagaatg taaagatccg cagaccatgg tgcctaaagc
     1861 gattaacagc gtgatttggc gtattggcct gttttacgtc ggctccgtgg tgttgctggt
     1921 tatgctattg ccgtggagcg cgtatcaggc ggggcaaagt ccgttcgtga cgtttttctc
     1981 taaactgggt gtgccatata tcggcagcat tatgaacatt gtggtgctga ccgctgccct
     2041 ctccagcctg aactcaggtc tgtactgcac cggacgtatt ctgcgctcaa tggcgatggg
     2101 cggttccgca ccgagtttta tggcgaaaat gagtcgtcag catgtgccgt atgccgggat
     2161 tctggcgaca ctagttgtgt atgtcgtcgg cgtattcctc aactatctgg tgccgtcgcg
     2221 cgtatttgag attgtgttga acttcgcgtc gctgggaatc atcgcttcat gggcgtttat
     2281 catcgtgtgc cagatgcgcc tgcgtaaagc aattaaacaa ggcaaagcag cggatgtcag
     2341 ttttaaactg cctggcgcgc ccttcacttc ctggctgaca ttactgtttt tactgagtgt
     2401 cctggtgctg atggcgttcg attacccgaa cgggacttat actatcgcgg cgctgccgat
     2461 tatcggtatt ctgctggtta taggctggtt tggtgtgcgc aaacgcgttg ctgaaattca
     2521 cagcactgcg ccagtcgtcg aggaagatga agaaaaacag gaaattgtgt ttaagcctga
     2581 aacggcgagc taattgcaaa attgcccggc tacgtgccgg gcaaatttag attactgacg
     2641 aaggtgtgcc gcatgcggcg tgataaaagt caggcaagtc tctccgtttt caccctcgcc
     2701 acatctatta cagcgcaata cgaatcacat cgtccggctg ggtagcttcc tgctgtttag
     2761 tggatttttg tttcacactg acatacagcg ttttgccatc ggcagacagc gccaggctgt
     2821 ttggatgagt cggcgtatcg aacgttttca ccactttata gcttttcgcg tcaatcacac
     2881 tgactttacc tgcctgacga tgcgttacgt aggcttcatt acgcgctggg ttaaacagca
     2941 cagccagtga ttctggtgcc gcaaccttcg ccagaatatt gccattacgg gtatcgacca
     3001 ctaacacttc tgcggcttta gaatcggtga taaatgcacg ctgcctggcg gtatcaaggc
     3061 taatgttgat aaagaagtgc tctttgccgt catccagcag ctttttacgg ctgaggattt
     3121 tattgtcggc ggtgtcgatg gtaatcaatt cgccgtcagc gttagtggtg taaagacgtt
     3181 tgcctttgct atccagcgcc agaccggtac tcattttacc ggtgttctgg atggcggttt
     3241 tcagtttgat attctcgcca tcaacgaccc aaatcacgct ctctttacca ataccactga
     3301 tgtaaacggt gttcgtggca tcatcagcta ccagctcacg cggttgcagc gggcgcacct
     3361 cttccgtgcg cttacgatca tccagcacca gacggccttt cacctcgccc gttttggcat
     3421 ctatcgccgt gaccgcgctg tttacggtgt taccaaacca caacgtctga gtcgtgttat
     3481 tgatggtggc accaaacggc ttgagatcgt tatggatcgc ctgcgtcact tccagagtga
     3541 ccggatcaag acgataaacc acgccacctt tatccagttt gcggctttgc gaagtggcga
     3601 gccacagtgc gttttcttgc tggctataag ccatttcgta ggcaccttta cctaccgctt
     3661 tacgcagcat ttcttctgcg gcctgcgtac tgaatgatga agcaacaagc aatgaaccta
     3721 acagtaatga accacgcagg cgcgatgaaa acagatgacg taaatgcatg acgactccct
     3781 ttgatgaacc gatatttttg ctgcttcaca atgcttcgct aagcgtaatc atcgttacgc
     3841 tcttgttata aatgggaatg gtaatcatta ttttctcaaa tgtggagcat ttcttgccgt
     3901 tacgactaca attttctcaa ttacttaacg tatcgtgccg tttcgttttc cataaattta
     3961 caatttcatt accataacgg cgttttccca ttcttaacct ggcttttttc atgaagattt
     4021 tttccgtccg acagaccgtt ttgcccgcac tgcttgtcct ttcccccgtt gtttttgccg
     4081 ctgatgaaca gactatgatt gtcagtgccg caccgcaggt ggtttcagaa ctggataccc
     4141 cagcagcagt aagcgtggtg gatggcgagg agatgcgcct ggcaacaccg cgcattaact
     4201 tgtccgaatc actgaccggc gtgcctggtt tgcaggtaca aaaccggcag aactatgcgc
     4261 aagatttaca gctgtcgatt cgcggatttg gctcccgctc cacttacggt attcgcggta
     4321 ttcgcctgta tgtggacggt attcccgcca ccatgcccga cgggcaaggg caaacatcca
     4381 acatcgattt aagcagtgtg caaaatgtgg aagtgctgcg tggccccttc tctgccctgt
     4441 atggcaacgc gtctggtggg gtaatgaatg tcaccaccca gaccggacaa cagccaccaa
     4501 ccattgaagc cagtagttac tacggcagtt ttggcagctg gcgctatggg ctgaaagcaa
     4561 cgggcgcaac gggagacggc acacagcctg gcgatgtcga ttacaccgtc tcaaccacgc
     4621 gttttacgac ccacggctat cgtgaccata gtggcgcaca gaaaaattta gctaatgcca
     4681 aactgggcgt acgcattgat gaagccagca aattaagtct gattttcaat agtgtggata
     4741 tcaaagcaga tgacccaggt gggctaacca aagcagaatg gaaggctaat ccacaacaag
     4801 cgcctcgtgc agaacagtac gacacgcgaa aaaccatcaa gcaaactcag gctgggttgc
     4861 gctatgagcg tagcctgagc gcgcaagatg atatgagtgt gatgatgtat gccggagagc
     4921 gagaaacgac ccagtaccag tcaatcccga tggcccccca acttaacccg tcacatgcgg
     4981 gcggcgtgat taccctgcaa cgccattacc aggggataga cagccgctgg acacaccgtg
     5041 gtgaactggg cgttccggtc acgttcacta ccggcctgaa ctacgaaaac atgagtgaaa
     5101 accgcaaggg ctacaataac ttccgcctga atagcggcat gccggagtac gggcaaaaag
     5161 gtgagttgcg tcgcgacgaa cgcaatctga tgtggaacat cgatccctat ttacagacgc
     5221 agtggcagct gagcgaaaaa ctgtcgctgg atgctggcgt gcgctacagc tccgtgtggt
     5281 ttgattccaa cgaccattac gttactccgg gtaacggcga tgacagcggt gatgccagtt
     5341 atcataaatg gctacctgcc ggttcgttaa aatatgcaat gaccgatgcc tggaatatct
     5401 atctggcagc cgggcgaggt tttgaaacgc cgacgattaa tgagctgtct tatcgtgctg
     5461 atgggcaaag cggtatgaac tttggtttaa aaccatccac caacgataca attgagatcg
     5521 gcagtaaaac gcgtattggt gatgggctgc ttagtctcgc attgtttcag accgacactg
     5581 atgatgaaat tgttgtcgat agcagtagcg gtgggcgtac gacttacaaa aatgccggaa
     5641 agacccgtcg tcaaggcgct gaactggcat gggatcaacg tttcgcagga gattttcgcg
     5701 taaacgcgtc ctggacctgg cttgatgcga cctatcgcag caatgtttgc aatgaacagg
     5761 attgtaacgg taatcggatg ccagggatcg cccgtaatat gggctttgcg tcgataggtt
     5821 atgtaccgga agatggttgg tatgcaggca cggaagcgcg ttatatgggc gatattatgg
     5881 cagatgatga aaatacggca aaagcgccgt cttatactct cgtcggctta ttcaccgggt
     5941 ataaatacaa ttaccacaat ttaactgtgg atttatttgg tcgtgtcgat aatttattcg
     6001 ataaagaata cgttggttct gtcattgtca atgagtcaaa cgggcgatat tacgaacctt
     6061 cgcccggacg aaattatggt gtcggcatga atattgcgtg gagatttgag taattacctt
     6121 gatgcccggt atttgccggg catttacttt aacgattgta ttgctggtat aaaatagcaa
     6181 tattttgttg cataatttca gcaagacaat gtctgcatcc ctctttgtct tttgctttta
     6241 acgcagcgag taacagatga tagttttcta tatgctcccg taattccgct ggattaatcg
     6301 cttcatagag ataatgtaaa ccaggcccca tcctgaccca cagttgctca atcatctcac
     6361 acaggatggg catatttgag cgatgataaa tcgctaagcg aaatagcctg tttacattaa
     6421 tgatttgttc catgtcgccc ttttcttgcg cctgctgtaa tttctcgagc aattcctgga
     6481 gttccgccag gtcttgcggg gtgaggtttt caacagccag agcaactgcc attaattcca
     6541 gttcgtagcg gatccgattg atttcgtcta attgacgttt ccccacttcc ggaactgtaa
     6601 atgcttgtgc aggtgcgaca gaaagcgcat tcaccgaaac cagacgtaat aatgcttcac
     6661 gcacaggtgt aatactcata cctaattgct ccgccagatt tttagtaatc aggcgtgcgc
     6721 caggttttaa tgcgccaata ctaagctgat gtttcaggtc gttctcaacc tgcagagtca
     6781 aactgacatg tttcattttt cccattccag gcattttaat tccttcgcgt gttttctgtc
     6841 caaccctgcc tgcacggcag aaggagttca cctggtgtat taaagtgaat aatcataaac
     6901 cacccacctt tgttgttatt agcagcagaa caatgacgtg gttagttaat taaagctgaa
     6961 aataagcttt ttgcctggat ttttatggcg gcttacgccg ccgttgaaac tttaatcatc
     7021 acccgcgacg cggatcacca ctttgccgaa gtttttaccc ttcagcaggc cgataaacgt
     7081 ctgtggcgca ttctctaaac catcagtaat atcttcgtgg tagtggattt tatcctcttt
     7141 cacccattgc cccatctcct tctgaaactc atggatgcgg tgaccataat cctgagcgat
     7201 aataaaccct tgcaagcgaa tacgtttttt cagcaccgtt gccatcaaca ggggtaaacg
     7261 atccggaccg ggtggtagct ctgtagcgtt atagctgctc actaagccac aaacgggaat
     7321 gcgcgcagat gtattaagta atggtagcac tgcatcaaat accttaccgc ccacgttttc
     7381 ataatagata tcaatacctt ttgggcacgc tttcaccagt tgttcggcaa aatcatccgc
     7441 gtggtgatca agacaaacat cgaagcccag cacctcgata gcatggcggc atttttccgc
     7501 gccaccggca acccccacca ccctgcaacc tttaagtttg ccgatttgcc ccaccgttgc
     7561 tcccacagga cctgtcgccg cagctaccac caacgtttcg ccctctttag gctgaccgat
     7621 atccagcagc cccatataag cggtaaagcc tggcatcccc agcacaccca gcgaccacga
     7681 tggattttgc ggatgatcgc caagtttcac cagatcatca ccactggata tgtcatagtc
     7741 ctgccatcca ctgtagccca gcacccagtc gccaggctga taatcaggat gattcgactc
     7801 cacaacacgg ctcaccgtgc cgccgaccat cacgccgcca atatcaacag gcggtgaata
     7861 agatggctca tcgctcatac gtccacgcat atacgggtcc agggacaaat aaactgtgcg
     7921 cagtaacacc tgtccttcac ccggtgtggc gacatcatct tcttcaagac ggaaattctc
     7981 cggaaccggt gcgccatgtg gacgcgaggc cagaacccaa cgtcgattac gctgcttttg
     8041 ttgccccata atgctctcct gtgcttttgg catgaatcca tcaatagtag caggctcacg
     8101 ggattggttt tgtttgcagc cgtcacatcc cctctgcaac acgcaataat ttcatcgagg
     8161 aatccgttgc ttcgggctgt tttcgatagt attgataaca tcatcatcat tattttatcg
     8221 aggccagaca tgtccatccg ttttgcccgc aaagccgatt gtgctgccat tgcggaaatt
     8281 tataaccacg ccgtgttgta tacggcggct atctggaatg accaaacggt tgatgcagat
     8341 aaccgcattg cctggtttga agcgcggacc atagcaggtt atccggtgct ggtaagtgaa
     8401 gaagatggcg tagtgacggg atatgcctcg tttggcgact ggcgtagttt cgatggtttt
     8461 cgccataccg tggaacattc ggtttatgtc catcccgatc atcagggcaa aggtctgggg
     8521 cgtaaattgt taagccgatt gattgatgaa gcgcgggata gcgggaagca tgtcatggtc
     8581 gccgggatcg aatcgcaaaa tcaggcctcg ctgcatctgc accattcgct gggatttgtc
     8641 gtcaccgcac aaatgccgca ggtgggcact aaatttgggc gctggctgga tctgacattt
     8701 atgcagttgc aactcgacga acgcactgaa ccggacgcga ttggatgaat cagtcgctca
     8761 cccttgcttt tttgattgcc gccggaattg gtctggtagt ccagaacacg ctgatggtgc
     8821 gcatcaccca gacctcctct accattctca tcgccatgtt gctgaactca ctggtgggga
     8881 ttgtgctctt tgtttctatt ttatggttta aacaaggcat ggcggggttt ggcgaactgg
     8941 tgtccagcgt gcgctggtgg acacttatac ccggcttgct gggatcgttt tttgtcttcg
     9001 ccagtatcag cggatatcag aatgtgggag cggcgacaac tattgcagta ctggtcgcca
     9061 gccagctaat tggcggtctg atgctggata tcttccgcag tcatggcgta ccactacggg
     9121 cgttgttcgg cccaatttgt ggcgcaattt tacttgtggt tggcgcctgg ttggtagcca
     9181 gacgctcatt ttaatcagag gatggtgccg cctttggtta actgttcgtg gcgcgcttcc
     9241 atatcttctt tatgcttgcg accgtgatga gcaatagcgg tgcgcaaacg ctgctgctgg
     9301 gtgtagcgat cttcccgact taactccgcg tcatcgctca gggcgattaa taattcgttc
     9361 atatgggcaa tgacgctctc ttcaatcgcg gcatccacgc gcgcgatgac ttcatccaga
     9421 tgcgacatac tttctcctta accacttgtg aaatttatcg attcatccaa atgatataag
     9481 tctgcgtggt gccgtcaagc cagtgagtaa gatttaaaac agtgaaaaca acagcgccac
     9541 cgggaaactc atcggccatg ttgcccccac cagaaaggcg cttaagaaac ggatgcgttt
     9601 cttatcgtga gaaagaaacc aggtgataag aaatgcgatt gctgccataa ccgcataaaa
     9661 aacaagcata tgttgataga gagacatgta cgggcgtcca gccagatgaa taaaacgcgc
     9721 gcaaatatgc gtcatttttt gacgtgcatc aagttttttc accagtttga gaaaaaatca
     9781 tcaaacagca acttgccctt atccatcttc agaatgagta ttattgcgga ttttcttaac
     9841 ccgtactata cacatagggg caatgataaa aggtggcaaa aatgaatgtt tccagtagaa
     9901 ctgtagtact gataaatgtc tttgctgctg ttggtttgtt tactcttatc tctatgagat
     9961 ttggctggtt tatttgatgt agtgattttg cacgcagcac gtactgtcag agaaatcaac
    10021 tctctgacag tacgcaagat cttctcatac tctccaggtt tacaacatta tccttaatat
    10081 ttacttcttt ttgctggcat ctacattgaa atgagtagaa aacatccccc tactgtcgtc
    10141 tatacaaaca gcttactgat tcaaacggaa tcttgttttc acatttttct atcatgaata
    10201 ctttatacgc gcgggagtct ataaatgctt tatactttca aggaataacg taaatataaa
    10261 aagatggcgt aaagtatatg catggcagga tgtttgcaac ctccttgatg acgctgcccc
    10321 attgaaattg caaaccccgc cctggttatc ttgcccattt atcatgcaaa acagataatt
    10381 ataggcctct gaatacctca gaaagattga attttttata gtccctctct tgaatatttc
    10441 catctgatat caatcgattc atctcttcaa caaaataatt tatatcatca cacttgttat
    10501 tatccggaag atctgagtag ttataattcc accattttat tctcaacagt tcatcaataa
    10561 catttgaatc aaacctaaat ctgattattt ttgcaggaac tcctgcaact atggcatatg
    10621 ggggcacatc tttggtaaca acggaattgg cagcaataac agcaccatca cctattgcga
    10681 tgccaccttt caatacaaca tcatttccta tccagacatc atttcctata atcggatttc
    10741 caggtttggt aatgaatggt ttcaatgtcc agctacgttc aaattcactc atagcaaact
    10801 tatcaaattc accgttataa gtcatcatgt gagtagtaaa acgattcaaa ggatgatttc
    10861 cacccattat ttttacattt gatgcaatac tacaaaatcg acctatacgt atattccctg
    10921 aaaaatggca ggtcggaaat gaaaaagcgc cgacactgga gaaatttttc acaggcattg
    10981 ttgaatattc ttcagcaata acattatctt caattgttat cttgtcacct tttgagaaaa
    11041 gaaataaatt cctttcttta tacgacttct tccccttatg atttaaaaag attttattat
    11101 ccatacaaaa ctcaaagtgt tttttcgtcc atctgaaggt aagggtttta tttttatctt
    11161 ccgtgaattt gtacaattga actttcattg ttaatacttc tacttataaa ttattagttt
    11221 aataacgata ctctaaagcc ttatgctaat caactgtgac aggatgaaaa atcgccatca
    11281 aacatttagc ctaattttta cgctagttat taatgtaatc attctaatac ttaaccgcca
    11341 ttaaactcag aacctaaaac gatagtggcc gtcctataac agccactaac atctaactta
    11401 atgtttaacc atgacgtggc ggacgacggt gtaatcctcc agcccataaa gtgacatatc
    11461 cttgccgtaa ccagaaagtt tctgtccacc gtgcggcatt tcacttacca gcatgaaatg
    11521 ggtattgacc caggtacaac catattgcag ccgtgcgctg acgcgatgcg ccctgcccac
    11581 atctttcgtc cataccgaag atgcaagtcc gtactggctg tcattcgccc agttcaccac
    11641 ctgttcttcg ttgtcgaaga gcgtaacact cactactgga ccaaatacct ctttttgcac
    11701 gatggcatcg tcctgtaatg cgccagccag cagcgtcggc gcatagtaat agccattacc
    11761 cttgcgcttt tcaccgccag tgatcacttt gatgtgccct gtcgctttcg cctcttctac
    11821 tgccttgctg acgcgttcga gatgcgccag cgagcttaaa ggtccaagct ccgtagactc
    11881 gtcatccggc gcaccagatt ttaacgttgc caccgcagca cccagttttt ccaccagcgt
    11941 atcgtaaatg cctttttgcg cgtagatccg acaagccgca gtacaatcct gtccagcatt
    12001 gtaatagcca aatgtacgta caccttcgac cactgcttca atatccgcat catcaaaaac
    12061 aatcactggc gctttgccac caagttccat atgagtacgc ttaatggacg acgcggtatg
    12121 gctgatgatg tgctcgccgg tggcgataga gcccgtcagc gacaccatcc gcactttggg
    12181 atgaccggtc agcggatcac ccaccgtttt gcctctgcca aacagtatgt taatcacgcc
    12241 tgccgggaag atatccttcg ccagctctgc cagcttcaac gcggtcagcg gggtaatttc
    12301 tgatggttta agcaccacgc aatttcctgc cgccagcgcc ggagccagtt tccacgctgc
    12361 catcatcagc ggataattcc acggtgcgat cgaagccacg acccccaacg gatcgcgacg
    12421 gatcatcgaa gtatgacctt caagatattc acctgccgcc agaccattca gacagcgcgc
    12481 cgcacccgcg aaaaagcgaa aaacatcgac aaccgccggg atttcatcat tgaacgcact
    12541 atgcagcggt ttgccacagt tacgggactc cagttcggca aaaacctgac cattttcttc
    12601 gataacatcc gccagtttca gcaggcattc cgcacgcgct ttcggcgtgg tttgccccca
    12661 ttcggcaaat gctgcatctg ccgcgcgcac agcagcatcc acctgctcta cggatgcctc
    12721 ggcaatctcc agtaaaacgt cccccgttgc cggattataa acaggctgtt tttccccttc
    12781 gccggtaacc agttctccgt taatcagtaa cttatgttgc atagcatatt cctgtatcag
    12841 tggttattga ccattgtcgc cttcgcgggt tagccaccag gcccccagga tcggcaaggt
    12901 tgttaccaac ataaccagca gtgccaccac gttggttacc ggtacatcac gcggtcgccc
    12961 aagctgattg agcaaccaca acggtaacgt tcgttcatga cctgccgtaa aggtcgtaac
    13021 gatgatttca tcgaacgaca aggcaaacgc cagcattcct cctgccagta gcgccgaact
    13081 gagattcggc aacactacgt agcggaaggt ttgccagcca ttggccccaa gatccattga
    13141 cgcctcaacc agactccacg aggtgcggcg aaaacgggcg atgacattgt taaacaccac
    13201 aactacacaa aaagtcgcat gacctaccac gatggtgaaa aatcccggct ccagattgat
    13261 ggttttaaag gcggttaata acgccagacc agtgacaatg cccggcagcg caatgggcag
    13321 cagcagtaac agcgaaatgg cgtttttgcc gaaaaagtct cgtcgccaca gcgcggcagc
    13381 tgccagcgtc cctaacacca gcgcaattaa tgtcgccagc gccgccactt taagtgacag
    13441 tgtcacggaa tcaagaatat cactacgctg tgctgccacg ctaaaccagc gcagcgtcag
    13501 gccctgcggt ggaaaactaa acgccgcatc ttcagtgtta aacgcatagg cggcgattat
    13561 caggatggga aaatgtagga aaacaacgcc gccccaggcc gccagtttga ggaaaaacgg
    13621 tgcgcgttca gagtgcatcg aacgctccca gacgtttcac gaacgccagg tacagtgcga
    13681 tgagaataat cggcaccagg gtgaatgccg ccgccatcgg catattgcca atcgccccct
    13741 gctgggaata aaccatattg ccgataaaat atcctggagg accaaccagc tgcgggacga
    13801 taaaatcgcc cagtgtgagt gagaaggtaa agatagagcc agcggcaata cccgggattg
    13861 ccagcggcag caccacatag cgaaaggttt gtcgtggacg tgcgccgaga tcagccgacg
    13921 cctgcaacaa tgacggcggc aaacgctcaa gcgccgcctg aacgggcagg atcatgaacg
    13981 gcaaccagat atagagaaac accagaaagc gccccagccc ggaagttgac agcgtatttc
    14041 cgcccaccgc aggtaatgta aggaacgcag tcagcagtgg ttccagccca agatgttgta
    14101 aaaaccactg agccacgcca tcttttgcca gcaataacgt ccaggcatag gctttaacaa
    14161 tgtagctcgc ccacatcggc aacattaccg caatataaaa aaacgctttc attttcccgc
    14221 tggtatagcg cgccatatac cacgccattg gaaaagccag aatggcgctg gcgatagtga
    14281 ctgccacagc catagtcagc gtgcggagaa tgatgtcgta attcgccgga ttaaacagcg
    14341 cacggatatt cgccagcgtc agttccggcg ttaccgacat ggtgaaatcg tcaaaagtat
    14401 aaaatccctg ccataacagt gtcagcagcg agccgaaata gacaatgcca aaccacatta
    14461 gcgggccaag cagcagtaaa aacagcccca gccccggatt acgccagaag aaaccggaca
    14521 ccttacccag acctggacgt gaaggtgatt gcaatacatt catcgccatt cacctctcct
    14581 caaccagcgg caccatcaca tcacgcgacc aggaaaccat cacctgttgt ccgggcgtga
    14641 gcgtggcagg cagttcttcg cctgtcatat tggcctgact caccagcagt ttttcaccgc
    14701 cgctcagtgt cagttcaaaa cgggtcgctg cgccctgata ttgcaccgcc tggatcgtgc
    14761 cattagcctg catttcgccg gggatattga gacggatatg ttccggtcgc agggcgaagc
    14821 ttcccgtcat gccgcaaaga ttctcggcca ttagcggatc aaaaacgttg gatgtcccga
    14881 cgaatccggc aacaaacggc gtgcgcgggc gcatatagag atcgcgcggg gaatcgacct
    14941 gctcaatgcg tccattattg aaaaccgcca cacgatcgga catcgataac gcttcgccct
    15001 gatcgtgggt aacgaagata aaagtgatac cgagagactg ttgcagtttt ttcagttcca
    15061 gctgcatctg ctcacgcaat ttgagatcca gtgcgccgag cggttcatcc aacagcaata
    15121 cgcgcggttc attcaccaat gctctggcga tagcaacccg ctggcgctga ccaccagaaa
    15181 gttgtgacgg tttacgttga tgtacaaacc ccaacgccac tttctccagc gcctcttgcg
    15241 ccattgcgtg ccgctgcttt ttattcacgc ctttgaccat cagcccatag gcgacattgt
    15301 caagaatcga catatgcgga aatagcgcgt agtcctgaaa gacagtattc acgtcccgct
    15361 cccacggtgg cagattgctg gcgggtttac caaagataga gatagccccg ccggaaagct
    15421 gttcgaagcc agcaatcagg cgcaggcagg tggttttgcc ggagccggac ggccccagca
    15481 tagagaagaa ctcaccatct tttatcgcaa tactgacgcc atctactgcg cgcacgtcac
    15541 cgtacaaccg cgagacgttg tcaaactcca ctgcgtacgt cataaagcac cccagcgaag
    15601 ttagcgaccg cccataatgg caatgtaatc ctgcgtccag cgactgtagg gaacaaactt
    15661 gcccccttct gctataggcg ttttccagaa ggcgattttg tcgaaatagt taaaaccgtt
    15721 ggtttcacaa cctttttcgc ctaataacgg actggcttta cacccttccg gcactaccgg
    15781 taacgagcca aaccaggccg ccacatcgcc ctgcactttt ggcgttaatg accagttcat
    15841 ccatttgtag gcgcaaaccg gatgtttcgc ttcgctatgc agcatggtgg tatcagccca
    15901 accggtaaca ccctccttcg ggaaaacggt agcaacaggc tggccttcgg ctttcagggc
    15961 gttggcctga tagggccagg cactggaagc aaccacaccc tcgtttttga aatcgctcat
    16021 ttgcacggta gtgtcatgcc agtagcgatg gatcaaactg tgttgagcgc gcagcacttt
    16081 cagcaccgcc tggtactgtt cttcggtgag ttgatacgga tcgctgatgc ccaactgcgg
    16141 ctgagtggct ttaacgaaca acgcagcgtc cgcaatgtag atagggccat cataagcctg
    16201 aacgcggcct ttattgctct tgccgtccgg cagattttgc tcaacaaaaa ccacttgcca
    16261 gctatccggc ggcgtcggga aggttttagt gttgtacatc agcaggttcg gcccccattg
    16321 gtaaggtgtg ccgtaaactt tgccgccaac attaaaccag tcgcctttaa ccacgcgcgg
    16381 atcgagcgtt ttccagttgg gaatcaatgc ggtattaatc ggctgcacgc gtttacccat
    16441 aatcaaacgc agcgaggcat cgccggatgc cgtaaccaga tcgtaacccc ctttggtcat
    16501 cagactgacc atttcatcgg aagtcgcggc ggttttcaca ttcaccgcgc agcctgtctc
    16561 tttttcgaac tgcgttaccc agtcgtattg tttatcagtt tgtccgcgtt cgatgtatcc
    16621 cggccaggcg ataatatcca gtcgcccttc cggtttatct aaattggtag gcggttcggc
    16681 ggcgtgagcg gtcattattg tcatgctgag cgcacacagg ctgctgcggg caaatgtctt
    16741 gctcataagg tcttactcct gtcgtaatta attgttcagc cgccgtgccg taaaaatatc
    16801 tccttgttta accatagacg gcggcttgca ggcacacctt cgcccctgag aaaatttaat
    16861 caggttgtga gctaccgcgc attatgccgt ctgtaaggtt atcttctgga gtatagtcaa
    16921 ggagttagga aatatgactg ctattcgaaa ttcatattac ccaatctaag aggaattaaa
    16981 ttaaacgcta ttacagccgt tcttgaataa gtttgcctaa ttgttttacc gcctgttctt
    17041 cacgctctcc ccactgccac gcggtattaa aacggaaaaa acgtgaccag ttttcaccgg
    17101 tagaaaacat tttacccggc gcaatactga tatgatgcgt cagtgc
//
LOCUS       DAZGSP010000108        17036 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZGSP010000108 DAZGSP010000000
VERSION     DAZGSP010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.109"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(23..388)
                     /locus_tag="ACHK74_003999"
     CDS             complement(23..388)
                     /locus_tag="ACHK74_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222733.1"
                     /translation="MNFISLWPLLLAQVTVMSLTVIFLAADRRFFSWDCFVVCSLSMM
                     MAEGIVIMKYELWHIPPALLGLAGVLSWLLLLLHTGYFSGISEGVHKRLWLAVASVFS
                     GAGMIIFYWLLQPSETIMK"
     gene            complement(402..1145)
                     /gene="traF"
                     /locus_tag="ACHK74_004000"
     CDS             complement(402..1145)
                     /gene="traF"
                     /locus_tag="ACHK74_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFP0222734.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKMKQPEPENKPAPVI
                     PRQEPDIMQKLTALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEQYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(1138..1395)
                     /locus_tag="ACHK74_004001"
     CDS             complement(1138..1395)
                     /locus_tag="ACHK74_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFP0222735.1"
                     /translation="MKVIFTSNRLVDFLIRLLITAIVISPVIIWSWDTVKETTAGGVL
                     AAAFVIFYSGVLLFILYFCFSALTDLQKPDERKSDERNEDE"
     gene            complement(1409..3259)
                     /gene="traN"
                     /locus_tag="ACHK74_004002"
     CDS             complement(1409..3259)
                     /gene="traN"
                     /locus_tag="ACHK74_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFP0222736.1"
                     /translation="MKRLLPLLLGLAVSVAQADSNSDYRAGADFARQIQGQGIGSIQS
                     FNPQESIPGYNANPDETQYYGGVTAGGDGGLKNNGTTQWATSETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQQINRSEFTNYTCERDTMVEEYCSRT
                     ARITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTVPVAGTVESASLSAHAGLYFL
                     NSRYDFMNTAFNVQLVTGQSGTFTIPVPSGFSVTQGQVLTGSGCTANGNCLPHGNGDR
                     MVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLKGTECSEPGGTKT
                     GVMEGKTWSITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGACLH
                     EYATYSCESRTSGKVMVCGGDVFCLDGECDKAHSGKSSDFGEAVSQLAALAAAGKDVA
                     ALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLT
                     VSVGEFCSRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKSPDCRGIT
                     VDELQRIKFDQLDFTNFYEDLMNNQKIPDNGALTEKVKEQIAGQLKQVGQ"
     gene            complement(3256..3678)
                     /locus_tag="ACHK74_004003"
     CDS             complement(3256..3678)
                     /locus_tag="ACHK74_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFP0222737.1"
                     /translation="MALTKKQREKLRMKFGGRCAYCGCELPEKGWHADHVQAVLRKSE
                     QCMKAAEKRIFRLKSTGDVFRPEADCPENLVPACAPCNLLKTTYSLEMFRTQVSLQVE
                     RGRRSSVNFRTAERFGLIQVVDKPVVFWFEQYQRESEV"
     gene            complement(3704..4075)
                     /locus_tag="ACHK74_004004"
     CDS             complement(3704..4075)
                     /locus_tag="ACHK74_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222738.1"
                     /translation="MSLPAGYYRIDPDIRALVAAMNVHGFRTYASCQGHGFPVTKLPP
                     YIAFACPVKMAALLEQRLRQDAESAIPRLTWGWSVKGTFNSDFQICFRLQPEGPHHWY
                     HRYCRRSLRADFRTLVRLVNP"
     gene            complement(4072..4710)
                     /gene="trbC"
                     /locus_tag="ACHK74_004005"
     CDS             complement(4072..4710)
                     /gene="trbC"
                     /locus_tag="ACHK74_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFP0222739.1"
                     /translation="MSRKIFVLFALTTGLFISAHASENVNTPENRQFLKQQETLSQQL
                     REKPDTQLQAWAEQQVRANPLQHSDNQFLDELVRKQQAAQKDKPQQGAVYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKATAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGHDIIRGNLRVRQALEKVVATGDCRQVAQGLLDGTGDKSQ"
     gene            complement(4737..5258)
                     /locus_tag="ACHK74_004006"
     CDS             complement(4737..5258)
                     /locus_tag="ACHK74_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222740.1"
                     /translation="MGFFSFLRKISDKEKGVFISEENWFSLLEKVSAFDKVRQWAESN
                     GGAYYPLQECRRLTDIEDIRKSEDILNRLEFIDRRFREFISIMSEYNCNDKSKDRWEK
                     WLSEYPPEKIYQVRREGDHLSLKNYGDDITLDRDTAVATVQITVSRKCITQEEMDELY
                     ALSERLKEKCAGN"
     gene            complement(5663..6661)
                     /gene="traU"
                     /locus_tag="ACHK74_004007"
     CDS             complement(5663..6661)
                     /gene="traU"
                     /locus_tag="ACHK74_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFP0222741.1"
                     /translation="MKRCMWLCVVLLLTGHVPAASADAACEGRFVNPITDICWSCIFP
                     LSLGSTKVSQGKVPDTANPSMPLQFCPAPPPVFKRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPEFGKTAQGTAKKEDKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASASAFNKPLDILFWCA
                     GSQGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMNTIGVDVAVCYEYPSPI
                     LPKARWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(6658..7290)
                     /gene="traW"
                     /locus_tag="ACHK74_004008"
     CDS             complement(6658..7290)
                     /gene="traW"
                     /locus_tag="ACHK74_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFP0222742.1"
                     /translation="MRCRGLIALLVWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKARVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RIFARQGEVMNPLQYVPFNQTLYFINGDDPEQIEWMKRQTPPTLESKIILVQGSIPEM
                     QKALDSRVYFDQNGVICQRLGIDQVPARVSAVSGDRFLKVEFIPVGEGGK"
     gene            complement(7287..7673)
                     /gene="trbI"
                     /locus_tag="ACHK74_004009"
     CDS             complement(7287..7673)
                     /gene="trbI"
                     /locus_tag="ACHK74_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFP0222743.1"
                     /translation="MTTTQKTTDVTARQRSHWWWTVPGCLAMVLLNAAVSYAIVRLNT
                     PVTVAFNMKQTVDAFFDSASQKKLSEVQSKALADRFNAALEASLQTWQQKHHAVILVS
                     PAVVQGTPDITREIQQDIARRMRAEP"
     gene            complement(7670..10300)
                     /gene="traC"
                     /locus_tag="ACHK74_004010"
     CDS             complement(7670..10300)
                     /gene="traC"
                     /locus_tag="ACHK74_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFP0222744.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEILGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTLGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAETQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASLQGAYITTQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLALVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLTRQNRARIDDVVDFLKNARDNDQYAESPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(10433..10780)
                     /locus_tag="ACHK74_004011"
     CDS             complement(10433..10780)
                     /locus_tag="ACHK74_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222745.1"
                     /translation="MKSIYLKYFLSFVSVGVGAMFICGLLYNDYLEQQPVTPEQLTEI
                     AQKTPCAAEAFKDTLNYQSEPLTLGKANKIASECRKRNEMEEARRVRENELKKIREKQ
                     LQALSDADSAKER"
     gene            complement(10811..11026)
                     /locus_tag="ACHK74_004012"
     CDS             complement(10811..11026)
                     /locus_tag="ACHK74_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222746.1"
                     /translation="MHTFIFLVLIKNINGGGSWNVTPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNKPLNNVMETLNGGG"
     gene            complement(11106..11579)
                     /locus_tag="ACHK74_004013"
     CDS             complement(11106..11579)
                     /locus_tag="ACHK74_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222747.1"
                     /translation="MHESEAEKIDSILDEHQQIYRRHNRIDNILAFCACIAWIPFIIA
                     AFGVDGIYLKILFAVLACCGAVCFFICFFTLLPESLMVLSRQRLVQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFTEGNKSE"
     gene            complement(11572..11793)
                     /gene="traR"
                     /locus_tag="ACHK74_004014"
     CDS             complement(11572..11793)
                     /gene="traR"
                     /locus_tag="ACHK74_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFP0222748.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQEKQRKHYA"
     gene            complement(11928..12443)
                     /gene="traV"
                     /locus_tag="ACHK74_004015"
     CDS             complement(11928..12443)
                     /gene="traV"
                     /locus_tag="ACHK74_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFP0222749.1"
                     /translation="MKKISLFVPLLGTLLLSGCAGTNSDFECDATTSDTCMTMEQANE
                     KAKKMEQPADAKPVAASLPRLADGNFRTTSVQAVAATTPSRSRPAVTALPEQKLLAPR
                     PLFTAAREVKTVVPVSSVVPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(12440..12712)
                     /locus_tag="ACHK74_004016"
     CDS             complement(12440..12712)
                     /locus_tag="ACHK74_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFP0222750.1"
                     /translation="MFISLMTEQSEITEIKMNMRNLNIIKSSPQSLPHNFNIAAVGLM
                     KKGMSMSLFSSPGHIMVVGQTCAGKANLTAELISAVVGNNKKGEGQ"
     gene            complement(12651..13211)
                     /gene="traP"
                     /locus_tag="ACHK74_004017"
     CDS             complement(12651..13211)
                     /gene="traP"
                     /locus_tag="ACHK74_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFP0222751.1"
                     /translation="MVGDALIFRVAFVVRWLLWVVRFVVIWPLATMALMALFVLWKDN
                     TTPGKLLVQEIDFVRQTAPAGQFPVRECLPSLHDLPGGAEKICRYRAADAADYVRETD
                     RSLMQLTTAFWATLALMYMSFAAGTGKYPMWPGKMKCVRVAAADERLKGVYTEDASLP
                     EKIRKCPVYFPDDRAIRNNGDKNEHA"
     gene            complement(13201..14655)
                     /gene="traB"
                     /locus_tag="ACHK74_004018"
     CDS             complement(13201..14655)
                     /gene="traB"
                     /locus_tag="ACHK74_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFP0222752.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQTPASPPGPEGEPQPGNTPVSFPP
                     QGTGAVPPPTAFYPGNGTTPPPQVTYQSVPVPNRIQRKTFSYDADRKKGPSLPYIPSG
                     SFAKSVLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVS
                     SERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVD
                     GIGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVI
                     PIGAGNEVTVVFQDGFQLKTVEEMALEQSQSQAEEGNAESPVPNPPTAESHLNGFNTD
                     QMLKQLGNLNPQQFMSGDRNQQGGDAGKEGGNGG"
     gene            complement(14655..15383)
                     /gene="traK"
                     /locus_tag="ACHK74_004019"
     CDS             complement(14655..15383)
                     /gene="traK"
                     /locus_tag="ACHK74_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFP0222753.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADSVWTGNHLKMVRFVVENKTRSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(15370..15936)
                     /gene="traE"
                     /locus_tag="ACHK74_004020"
     CDS             complement(15370..15936)
                     /gene="traE"
                     /locus_tag="ACHK74_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFP0222754.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLAVNVIQGINNYRLQNE
                     QRTAVIPMGFNVPFAVSQNSADASYLQQMALAFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNQMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(15958..16269)
                     /gene="traL"
                     /locus_tag="ACHK74_004021"
     CDS             complement(15958..16269)
                     /gene="traL"
                     /locus_tag="ACHK74_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFP0222755.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAGICIGWGMMT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(16284..16649)
                     /gene="traA"
                     /locus_tag="ACHK74_004022"
     CDS             complement(16284..16649)
                     /gene="traA"
                     /locus_tag="ACHK74_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFP0222756.1"
                     /translation="MNAILSVQGASAPVKKKSFFPKFTRLNMLRLVRAVIPAAVLMMF
                     FPQLALAAQGAGQDLMAGGNATVKATFGKDSSAVKWVILAEVLVGAIMYMMTKNVKFL
                     AGFAIISVFITVGMAVVGL"
     gene            complement(16682..16909)
                     /gene="traY"
                     /locus_tag="ACHK74_004023"
     CDS             complement(16682..16909)
                     /gene="traY"
                     /locus_tag="ACHK74_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFP0222757.1"
                     /translation="MSRNIIRPAPGNKVLLVLDDATNHKLLGARERSGRTKTNEVLVR
                     LRDHLSRFPDFYNLDAIKEGTGETDSIIKDL"
BASE COUNT         4073 a         4808 c         3909 g         4246 t
ORIGIN      
        1 tcatttacat attaacaggg cattatttca ttatggtttc tgatggctgg agtaaccagt
       61 agaatataat cattcctgca ccagaaaaca ctgaggcaac agcgagccac aaacgcttat
      121 gcacgccttc tgatatgcct gaaaaatatc ctgtatgaag tagcagcagc aaccatgaca
      181 acactcctgc cagacccagc agggcaggag ggatatgcca cagttcatat ttcataataa
      241 caataccttc tgccatcatc atggacaggc tgcaaaccac aaaacagtcc catgaaaaaa
      301 aacgcctgtc agcagcaagg aaaatcactg tcagtgacat cactgtcacc tgggccagca
      361 ataaaggcca taaagaaata aaattcattt aaacgacctc tttaaaaatt cggtttaaag
      421 tcttcagaaa cgttcaggaa ctgttttgcc aggtcgtcct gagaaataaa gccgtatgat
      481 aacgggcgaa cactgccctg tttcgggtca accagcatca tggccgggaa atatttcaca
      541 ccgaggcgct gcgcctgccc ctggtcagtc cgggaatccg gcaacagtgg attaatcacg
      601 ccatccacgg aaacgggaat aacactcaga ccatacgtat cccggaagcc attaatgacc
      661 tgcgccagct gcccgtcgat ggggtcctgc ccccggtaaa aaaacataat gccgtattgt
      721 tcagccagtt tcgcaatggc ctgtcgctgc tgtgcctggt ctgccgccag ctggttccgt
      781 accgtgccgt tgtaatggct gtactgcagg ttatagtcca gttcaggatg tgccagcatg
      841 gcctttttgg cgctcatggt gaaaagcccg gcctgctgag tccagtaatt ctgcagccgg
      901 aaatatttca caaaattatc cactccggga tacagaatgg cttcgtacag cgcccgcttt
      961 gttgccgtct gcagcgcggt cagtttctgc ataatatccg gctcctgacg gggaattaca
     1021 ggtgcaggtt tattttccgg ttccggctgt ttcatctttt cgttatacca ctgccagcct
     1081 gcatctttcc ctgacgccgg aaaaataaag cagcagagta acagtggcag taatgcttta
     1141 ttcatcttca ttcctttcat cactttttct ttcatcaggt ttctgcaggt cagtcagagc
     1201 agaaaaacaa aaatacagaa taaacaggag cacaccggag taaaaaataa cgaaagcagc
     1261 agcaagcacc ccgcctgccg tggtttcttt taccgtatcc catgaccaga taattaccgg
     1321 ggaaatgaca atcgctgtaa tcaaaagccg gataagaaaa tcaacaagac gattcgacgt
     1381 gaatatgact ttcattattt tcccgacctt actgccccac ctgtttcagc tgaccggcaa
     1441 tctgctcttt cactttttcg gtcagcgcac cattatccgg gattttctgg ttattcatca
     1501 ggtcttcgta gaagttagtg aagtccagct ggtcgaactt aatacgctgc agttcatcca
     1561 cggttattcc ccggcagtca ggactttttg cgctgccaaa cccgatacgc aactgcccgt
     1621 tacgcccctg ctgctggact atctgcgcca gcttcgaatc aaactggcag taactgcgct
     1681 ttttctgcag acagacaccc agcactttcc gggagcagaa ctcgcccaca ctcaccgtca
     1741 gtttgttgtc ctttgccttt cccagcgcct tttcttcgct gttgcacttc gccagtccca
     1801 catcctgacc ccagccacca tccttacagc agttactgaa gccggctgcg gccttacggc
     1861 agaatttcgc ctcgcccgtg aaagccctga catccacacc attcagggcg gcaacatcct
     1921 taccagcagc ggccagtgcc gccagctgtg atactgcctc accaaaatca ctgctttttc
     1981 cgctatgcgc cttgtcgcat tcaccgtcaa ggcagaagac atcaccacca cagaccatca
     2041 cttttccgga cgttctggac tcgcaggagt acgtggcata ctcatgcaga caagctccct
     2101 catcggaata aaacgcgcac tgacgtgagg ccagcgtaca ggccggatta tcgacatatt
     2161 tctgacaggt gccgttatct gcggactgtg tgacatattt atcccggtat gcccagcacg
     2221 cctgtgtgat gctccaggtt ttaccttcca tcacacccgt tttcgtacca cccggttctg
     2281 aacactcagt gcctttcagc acgccatccg ctttattaaa cggacaactc tccacccact
     2341 ccacccgtgg aacccattcc ttatcacgga ccttcatcct gagtttcagc gtaaaggtgg
     2401 aagcaccact gacaagcgat tcatagacca tcctgtcacc attcccgtgc gggaggcaat
     2461 taccgtttgc agtacagcca ctaccggtca gaacctgccc ctgtgtgaca gaaaacccgg
     2521 agggcacagg tatggtgaaa gtcccgctct gtcctgtaac cagttgcaca ttaaaggctg
     2581 tgttcataaa gtcgtaacgg gagttaagga aatatagccc tgcatgagcc gacagcgagg
     2641 cgctttccac tgtcccggca acaggtacgg taacgctgaa caccagtttc agaccgttca
     2701 tactgaaccg gaactgtgag tgcggtatcg tcacctcacg atattcatcc gtgtatttcc
     2761 agtctcctgt gatcctggct gtcctggaac aatattcctc caccatcgtg tcacgttcac
     2821 aggtatagtt tgtgaattca ctgcggttta tctgctgcgc actgcactgc tgtccggtat
     2881 ttcccacgat actgtcagcc cggttcacca catccctgcc ggtctgaata aacggcgcat
     2941 ccggtgacag aatgtctttc ggcttgttca tgaatgactc tgtgatggtt ttgccggttt
     3001 cactggttgc ccattgtgtg gtgccgttat ttttcagacc gccatccccg ccggcagtca
     3061 ccccaccgta atactgcgtc tcatccgggt tcgcgttata cccggggata ctctcctgtg
     3121 gattaaaact ctgaatactg ccaataccct gcccctggat ctgacgggca aaatcagcgc
     3181 cggcacggta atcactgttg ctgtcagcct gtgccacact gactgccagc cccagcagca
     3241 atggcagaag acgtttcata cctcactctc cctctgatat tgctcaaacc agaacacgac
     3301 tggtttgtcc accacctgaa tcagcccgaa ccgttctgcg gtcctgaaat tcacgctgct
     3361 tctgcgcccc cgttcaacct gcagggacac ctgcgttctg aacatctcca gcgaatatgt
     3421 tgttttcagc aggttgcacg gcgcacaggc ggggacgagg ttttccggac agtcggcctc
     3481 cggtctgaaa acatcaccgg tggatttcag cctgaaaatg cgcttttctg cagccttcat
     3541 gcactgctct gacttgcgga gcaccgcctg aacatggtcg gcatgccatc ctttctccgg
     3601 cagttcacag ccgcagtacg cacaacggcc accgaatttc atacgcagtt tttcccgctg
     3661 tttctttgtc agcgccatca atgcctcctt aacaggaaca gatttacgga ttcactaatc
     3721 ggaccagcgt cctgaagtct gcacgcagtg agcggcgaca gtaccggtga taccagtgat
     3781 gagggccttc aggctgaagc cggaagcaaa tctgaaaatc actgttaaaa gtccccttca
     3841 ccgaccatcc ccatgtcaga cgtggtattg ctgattcagc atcctgccgc agtcgctgct
     3901 ccagaagggc agccattttt accggacagg caaaggcgat ataaggcggc agctttgtaa
     3961 cagggaaacc atgcccctga caggacgcat atgtacgaaa tccgtgaacg ttcatcgcgg
     4021 ccaccagtgc acggatgtcc gggtcaatcc ggtaatagcc tgcaggtaaa ctcattgcga
     4081 cttatccccg gtgccgtcca gcagtccctg agccacctgc cggcagtctc ctgtggcgac
     4141 cactttctcc agcgcctgtc tgacgcgcag atttccccgg atgatgtcgt gcccctggct
     4201 gcagaacacc accagagccg gcacgctgcg gatgccgtac tgtgaaaaca gcgtcgggtc
     4261 gatctgaaca ccatcggtcg caccgtcttt caccagagac agcactgctt cagccgtggc
     4321 tttcaggtcg ttgttcacca taccccgcag tgtggccgga ataccgtagt gccgggtttc
     4381 gcccagcatc cgtttcagcc cctcttcggg tatggaaaac gacacaaaat acacggcacc
     4441 ctgctgtggt ttatccttct gagcagcctg ctgtttacgg accagttcat cgagaaactg
     4501 gttatcggaa tgctgaaggg gattcgccct gacctgctgt tctgcccatg cctgcagctg
     4561 tgtgtccggc ttttcacgta actgctgact cagtgtttcc tgctgcttca ggaactggcg
     4621 gttttcgggt gtattcacat tttccgaagc atgggcagaa ataaacagtc ctgtggtcag
     4681 ggcaaaaagt acaaatattt tccggctcat atcatctccc ggcatagtga tataatttaa
     4741 ttaccggcgc acttttcttt cagtctctca gacaaagcat ataattcatc catttcttcc
     4801 tgagtaatgc attttctgct gacagtaatc tgcaccgtag ccacggcggt atctctgtcc
     4861 agagtaatat catcaccgta attttttaat gacagatggt ctccctctct ccggacctgg
     4921 tatatttttt ccggtgggta ttcagacaac catttctccc acctgtcttt agatttatca
     4981 ttacagttat attcagacat aatgctgatg aactcccgaa atcttctgtc aataaactcc
     5041 agtcggttca gtatatcctc tgatttcctg atatcttcaa tatctgtaag ccgacggcac
     5101 tcctgcagag gataatatgc cccaccatta ctttcagccc attgtctgac cttatcaaaa
     5161 gcactcacct tttccagcag tgagaaccaa ttctcctcac tgataaaaac ccctttttct
     5221 ttatcactta tctttcgtaa aaatgagaaa aatcccataa cgcaccttta cttatattgt
     5281 acacatgcac acactgttat atttatgata accatcagac tcatttcctg cgcttaacgg
     5341 gcataaactt atctgaagaa agatatgaaa taacaacgcc caccactgta agaataaaag
     5401 acgtcttcag atacagtctg acaagcccga ttttttccag tgtatctgca tcatgccagt
     5461 cattaacaat gtctgagaga atatattcag ggcaaaaata aaaccacacc acagagcaca
     5521 tcaccatagc aaaaggaaac agctttaata cattgctcgt taaccggata aacagaagac
     5581 atatttttct gaagtcatct gcgtgtttat ttcttattac ccacaccttc atataatcga
     5641 tcctcttatt gattaacatg acttacagga acacacagtt acgtttacgc cacatcaggt
     5701 aaccgaagtt tttcttagtg ttgggcggat tttttcccgt ctcccagcgc atcacactgc
     5761 gtccgaacgg atggcactgc ccgctgtccg ggtacatatt caccatctga taacgccagc
     5821 gcgctttggg aagtatgggc gacggatatt cataacagac agccacatcc acgccgatgg
     5881 tgttcattat cattccctga cggtggagct taaacgccat ccgttcactg accagcaggg
     5941 atgactgcag cggactggat tcattgctca cccagccatt aaacggatac atactgccct
     6001 gcgaaccggc acaccagaac aggatatcca gcggcttgtt aaaggcactg gcactggcaa
     6061 tggcatccgc cgcacaggct ccctgtgcta tcggatttgc aaaaatgacc gcctccggat
     6121 tgaggatggt ggtcaggctg ctgtccgtcc aggtagggtc aatttctgac agataggcaa
     6181 tatccaggtc tccgccttcc aggcacccca acgacgtgat gatgttcagc cagtacgtca
     6241 gcgggtattt gtaccagtga acgtgataga acgccccgtt cacctgttta tcttcttttt
     6301 tagccgtacc ctgtgccgtt ttcccgaatt ccggcagact gaaccccaga ttaaccatgc
     6361 atcccggtga gcgggtgaca tccgtcagtg ccatcggctc ccagtaacca atagccagcc
     6421 cgatacgttt aaaaacaggg ggaggcgccg ggcagaactg gagcggcatt gacgggtttg
     6481 ccgtatccgg cacctttccc tggctcactt ttgtgctgcc caacgagaga gggaagatgc
     6541 agctccagca gatatccgtg atcgggttaa caaaccgccc ttcacaggca gcgtccgctg
     6601 acgctgcggg aacatggcca gtcagcagca aaaccacaca caaccacata caccgtttca
     6661 tttccctccc tccccgaccg gaataaactc caccttcagg aaacgatcgc cactgacagc
     6721 actcacccgt gccggcacct gatcaatgcc gagacgctgg cagatcacgc cgttctgatc
     6781 aaagtaaaca cgactgtcca gagccttctg catctccggg atactgccct gcaccaggat
     6841 aattttgctt tccagcgtgg gaggcgtctg gcgcttcatc cattcaatct gctcaggatc
     6901 atcaccgttg atgaaataca gtgtctggtt aaaaggcaca tactgcagcg ggttcatcac
     6961 ctccccctgg cgggcgaaga tacgaccttc gttatcccgg atatccgcag ccagtctgac
     7021 cgacgggtca aacaggcggc tgctgtattt ctccgtccga ccgataccgg gcacggcagg
     7081 gggccgcagg ctgttgcgga tcacccgcgc cttaaacgca tccattttcc ggcccatctc
     7141 tccggactgc tccagagctg tcagacgctg cataatcacc gtcagcatgt ccggctcctt
     7201 taccggccac aaatcccccc aggtgccaag atcagctgca gccacactct gcccccatac
     7261 cagcagggca atcagccccc ggcatctcat ggttctgccc tcattcgccg ggcaatatcc
     7321 tgctggattt cacgggtgat atccggtgtc ccctgtacca cggcaggcga caccagaatg
     7381 acagcgtggt gcttctgctg ccacgtctgc agactggcct ccagtgccgc gttaaaacgg
     7441 tcagccagcg ccttcgactg cacttctgac agttttttct ggctggcgct gtcaaaaaac
     7501 gcatccactg tctgtttcat gttgaaggcg acggttaccg gcgtgttcag tctgacaatg
     7561 gcgtaactga cggctgcgtt cagtaacacc attgccaggc atcccggcac cgtccaccac
     7621 cagtggctgc gttgacgggc tgtaacatca gtcgtttttt gcgttgtggt catgcaacac
     7681 tcctgtattt ctcatgttct tccagccagg cttccagcga agccatctcc ggccctgact
     7741 tcttccacgc cagctgccac accgcctcat gaatactcag cccctcctta cgtttctgct
     7801 gcacaaactc aaaatccggg ccgtcagagc tgtacatggc acggcttaac gggtccacaa
     7861 acagacggtg ccaggaggaa tggttttcca cctgcagcag gaaggaactg aaccactggt
     7921 ctttggccgc gccaaacttg ccaatcatgt cacgctgcag tggcaggaac tggtccggat
     7981 acagctggtt gtatttcgcg aactccttcg cactctgttt gaggataatt ttgtaggagg
     8041 agttacccca tgccgcacgg gcagcactgg aggccttgtc agagtcaaag tcgacgatgt
     8101 tctgtgtgat ggtgatatag gcaccggtat gacgacgggc ggtacggtaa cctttctcaa
     8161 taaactcacc gaccttgtga tttttgaagt ccagcagacg ccagccttca tcaataacgt
     8221 tcagtttctt gaggttacgc ggcgtgcggt acatccggtt ctcgatgtaa atgataaggc
     8281 tgaacatcac cgcaaccagc agggacggac ggtcttccag tccacccagc tccagcacca
     8341 ccattttcgc atcatcccgc agggacggtt catcagagtt aaaatactgg ccgtaagtac
     8401 cgttggcagt gtactggtcc agcagcacta tcatttcatc cagacggctg cggatggtcg
     8461 gggactcggc atactggtcg ttatcccggg cgtttttcag gaaatccacc acatcatcaa
     8521 tacgcgcccg gttctgtctg gtaagccagg aggccctgac cgcctgcagc agcagacctt
     8581 catgcacttc atccaggtta ccgttggggc tggccatcac cgacaactgg tcacggacac
     8641 gctccgctga ctggtcaata tcggtgatgt tcgcaaacgg attaaagcgc agggtttcac
     8701 cgtccagata tacgcccccc atgttctcac acagggactt gtagccatcc cccatgtcga
     8761 acaccacggc aaagcccccg gagtccagca cgctgcggat aagtggctgt atcagcccgg
     8821 ttttaccggc cccggaggtg ccacagaccg ccatgttgta gttggtgtta ttcatccccc
     8881 ggaagaaaat atcgataaac gccagctggt tacggtaggt gggtgccagc agacctgccg
     8941 gtgtcagggg gttatccgcc actaacggca tcaggttggc cacattaaag ctctctgcgc
     9001 gctgaaccac accggcctct ttcagctgtt taaacagccc tttcccggcc ataaagggca
     9061 ggcaggtcag aaaattgcgc atgtggttaa agcgcggtga aatcagctca aaaccgtttt
     9121 tacggaagct gttcaggatg tcctgctcca cttccagcgc cgtttcattg ttgtccttgc
     9181 agaacgctgt gatgttgagg aagtaggaca ccacggagga ctgacctgag cccagccgct
     9241 ggcgcagttc cccccactcc ttcgcttctt tctccacgga cggaaaccat ttggcatagg
     9301 aggtcttcga cttcttctcc aggtccatgt acttcaggtt ggcttcgctg tgggttttca
     9361 cctggtcttc caccaccagg gccagcgtca ggatgaacgg gcaggaaatg gacatttccg
     9421 ggttcagcag gttgctgtag ttgtcggcca tgttccacag gaaggcgatt tccgggttac
     9481 gggccagatg gaaattcagg atgcgggccg tgctgttcct gccgttctca cgcaggccca
     9541 gcgtcaggta atcagcccgg actttcagat caaaactgtc ctccacacac tgataattca
     9601 aatcagaata cgggtccagc tgacgtcttt tcgggtacag ggagtccggg ttatggttaa
     9661 tcatctcccc gacaatatcg atgaaagcct gtgcatccac cgtctgcgtg gtgatatatg
     9721 ctccctgcag agacgcccgg atgattttca ccaggttttc catttccaga atgtcggccc
     9781 ggcttttttt cttcgaggga gaacagtacg agataaacac ccggtaatgg cgcagggtca
     9841 ggggcagatt cattccctcc ggcagcggaa actgtgtttc tgcggctttc atataatagg
     9901 cacgggtgat tgcgttaaac cgctcagcct gctcaccgga ccaggagaac tcccgcaggc
     9961 cgtattcaat cctgtcacca accagctgac tggacatcag atggatacac agcggaatac
    10021 cgcgcggcag tttggtacgc agcatgtggt cgagcgcctc cacaatggac tcattcgccc
    10081 cattaatggg aatggcttcc agcataaagc ccagcgtggt gtcattcatg aacagaccag
    10141 attcctggtt gtaatcccgg tacggcagca gacggctgaa ctgcgggaag ctcatttcac
    10201 ccagtatttc attggccttt gcggattcat caggcagttt cagtgcggtg acaagggagt
    10261 tcaccgcctg agtgacggcc tcaagtgggt tattactcat acgagtattc cttagtacgt
    10321 ttaaaaaagg aaaatatcag tctgtggccg tggatatatc acgcagtgcc attcttaaaa
    10381 ccacagcaca aaataagaaa tggaggcttt taacaggaaa ccaggattac aattaacgtt
    10441 cctttgctga atcagcatca cttaatgcct gtagttgttt ttcgcgtatt tttttgagct
    10501 cgttttctct tacccgtcgc gcctcttcca tttcatttct ttttctgcat tctgatgcaa
    10561 ttttatttgc cttaccaagg gttaaaggtt cagactgata attaagtgta tccttaaatg
    10621 cttctgctgc acagggagtt ttctgtgcaa tttctgtcag ttgttctggt gttacaggtt
    10681 gttgctcaag ataatcattg taaagaagac cacatataaa catagcccca acaccaactg
    10741 atacaaaaga cagaaaatat tttaaatata tcgacttcat ttcattttcc ttcgaagcag
    10801 taagcactca ttacccacca ccattcagtg tttccatcac gttgttcaaa ggtttatttg
    10861 ttttaacttc aatacattcc cctgtatttc caaatcccct gcctccatgt gcttcgattg
    10921 tccgaagaac ctgcacgcat acgtccgtat ttggcatggg ggtaacattc cagcttccgc
    10981 caccgttaat attttttatc agcacaagaa aaataaaggt atgcattctt tattacctcc
    11041 gcttgtatat taatcactga atatcccttc agttaactta tgaggtcata aaacactgca
    11101 gattctcatt cggatttatt cccttcagta aacttccgga tggtatcctg ttcacgctga
    11161 cgggtggcag actcctgcat tgcatccact ttctcctgcc agagacgccg gatatccttt
    11221 tcatcccgac cagtcagttt tttaccggac agcagacggt taagcagttc ctgtctcgca
    11281 tccggtacgt cttcagtcag acgcataagc tgaaccagac gctgacggct gaggaccatg
    11341 agtgattccg gcaggagggt gaagaagcaa ataaaaaagc ataccgcacc acagcaggca
    11401 aggaccgcaa acaggatttt cagatatata ccatcaacgc cgaacgcagc aataataaag
    11461 ggaatccatg caatacacgc acaaaacgcc aggatgttat ctattcggtt atgtctgcga
    11521 tatatctgtt gatgttcatc cagaatagaa tctatttttt cagcttctga ttcatgcata
    11581 gtgtttcctc tgtttttcct gatatgcctg acattcaacg cataaagtga cacctggaaa
    11641 tattttccgc cgggcttccg gaataggatt tccgcatgct tcacagagat aaacaggaat
    11701 accatgagca tttattttct ggcgtatccg gtttattcct gtcatggtca gttgttctgt
    11761 cactgaatat gcttcatcgg cttcatcact caaggaatat tcctcccgac atgttctgac
    11821 acgctaccgc ccatcccttt tcacgcacag ggcactcgtt ttacaggcag attttgaata
    11881 cagaaagccc gccggcattg ccggctgata tacaacttca gggcctgtta attaatacgt
    11941 ggttttcccc acgcagacgg tttaataaca aaaaacacgc tggatggctg atgataaaca
    12001 tcctgattat caatatacgg cgctatccat aatgccgccg tctgttcacc cgttcttagc
    12061 ggacgtgggg gtgttaccgg cacaactgaa ctgaccggaa caaccgtttt cacttcccgg
    12121 gcagcggtaa acagcgggcg tggtgccagc agtttctgct ccggaagtgc tgtcacagca
    12181 ggcctgcttc gggacggcgt tgtcgccgca acggcctgca ccgacgtagt cctgaagttc
    12241 ccgtcagcca ggcgcggcag tgatgccgca accggctttg catctgcagg ctgttccatc
    12301 tttttggctt tttcgttggc ctgttccatt gtcatgcagg tatctgacgt ggtggcatca
    12361 cactcaaaat cagagttcgt tccggcgcag ccggaaagta ataaggttcc cagcagagga
    12421 acaaataaag aaatcttttt cattgccctt ctcctttttt attattcccc acaacagcag
    12481 aaattaactc tgcagtcaga tttgctttcc ccgcacaggt ttgtccgaca accataatat
    12541 gccccggaga agaaaataaa ctcatcgaca tccccttctt catcaaaccg acagcggcta
    12601 tattaaaatt gtgcggcagt gattgaggac tgcttttaat aatattaaga ttacgcatgt
    12661 tcatttttat ctccgttatt tctgattgct ctgtcatcag ggaaataaac agggcatttc
    12721 cttatttttt ccggcaggga agcgtcttcc gtataaactc ccttcagacg ttcatctgcg
    12781 gctgccaccc ggacacattt catttttccg ggccacatcg gatatttccc ggtgcctgcc
    12841 gcaaacgaca tatacatcag tgccagtgtt gcccagaatg cggtggtcag ttgcatcagt
    12901 gagcggtcag tctccctgac ataatctgca gcatcagcag cacggtaacg gcagattttc
    12961 tctgctcccc ccggaaggtc atgcagagaa ggcagacatt ctcttacagg aaactgcccg
    13021 gcaggggctg tttgtcttac gaagtcaatt tcctgaacca gtaattttcc tggtgtggtg
    13081 ttgtccttcc ataaaacaaa taacgccatc agggccattg tcgccagcgg ccagataacc
    13141 acaaacctga cgacccacag cagccagcga acgacaaacg ccacacggaa tatcagtgca
    13201 tcacccacca tttccgccct ccttaccggc atccccgccc tgctgattcc tgtctccgga
    13261 cataaactgc tgcggattca ggttgcccag ctgtttcagc atctggtcgg tgttaaaacc
    13321 gttcaggtgg ctttctgccg tgggcggatt cggcaccggg ctttcagcgt tcccctcctc
    13381 cgcctggctc tgtgactgct ccagcgccat ctcctccacg gttttcagct ggaagccatc
    13441 ctggaacacc accgtcactt cattgcccgc accaatcggt atcaccgggt gatactgctc
    13501 agcacgtttg atgtagtagt cactgagcgt ctgtgccgct ttcgatgcac cgccgccgat
    13561 acccattttc aggacatcgc cggccccgta agcggcagtg gcccccagcc cgacagccgg
    13621 ctgggaggca cgctccattc cctgaccgat accgtcaaca aatcccgcgc cccatgccca
    13681 gccgaggatt ttgccgttac gcatcaccac ttcgcccttg ataccgtttt taccccggaa
    13741 gctgacatgc cccttaatcg gcatatcaat agttttgccg tccttcaggc agctgatatt
    13801 gcgggtgcgt acaatggcac gctcactgga cacatccccc caggcttcca gacccacaaa
    13861 acatcccgtg gcgtcatacg ttttgctgtt cggcatttcc accaggccgg tgatacgcag
    13921 ctgcatcggc acagtggatt cattaccggt gacagacgca ttggcatctg ccccctcaat
    13981 caggacggat tttgcaaagc tcccggacgg gatatacggc agggaggggc ctttctttct
    14041 gtcagcgtca tagctgaacg ttttacgctg aatacggttc ggtaccggca cagactggta
    14101 tgtcacctgc ggaggcggtg tcgtgccgtt ccccggataa aacgccgtcg gcggtggaac
    14161 agcaccggtg ccctgcggag ggaaagatac gggtgtgttt cctggctgtg gttcgccttc
    14221 cggcccgggc ggtgaggctg gcgtctgcgg tacaggctca ccggtcgcgg tgacgggatt
    14281 tgcacccagg gcttttacct gctctgccag ggcggcattg tcctgtccca gcttttcaat
    14341 acgacgctgg tcatcaccac gctgtttgtt caggacatcc agttcacgac ggatttcctc
    14401 atactgcttc tgcatctgcg ctgccgtcac ctgcatctct gtggtggcat gctgacgcac
    14461 cttgtcatca aaggtcgtat ccaccacacc ggtcatatcc ggcacaggct cctgttcagc
    14521 cacggtttca ccgttaccgg acatgtccac atcagacaga tacagtgccc caccaatcgc
    14581 ggaggctgta ccgacaacca caatccccag ccacaggtac tgcttgcgtt tcacaatggt
    14641 attgatactg gccatcagtt gccctccccg tcacggatga catacacatc catgcgcgca
    14701 cctgccagta actgactggc aggctggctg aacatcacgg cacgggttcc cggctgccag
    14761 aaatcacttt cccggatatt cagggcagac cgcgttttat tttccacgac aaatcggacc
    14821 atcttcaggt gattccccgt ccatacggaa tctgccactg aagacagccc cgccggggcc
    14881 tgcagggttt cctttgtcac aggaacctga taccagccgg cgggtaattt tccgccacgg
    14941 acagcctggc tgatggtcac aagcagggat tcgtaaggcg tggacgtttc ccacgcgccg
    15001 gcttcttctc cggttccgcg caggtcactg accagctgaa tggtacgccc tgcgccttca
    15061 cggggaacgg cctgaatgga aaggttaaga ccacgttctg tttccagaat gaacgtaaaa
    15121 ggctttttgt tgacggtggc caccaccaca ccaccggagg cggtctgctc attattggtc
    15181 agggcaccat ccagactgtt cacggcgata atccggtcac caggaacggt aaacagattc
    15241 gggctggtat tgctgatggc aatactggcc tgaccaccgt tcaccattgg caccacggta
    15301 ggggccagcg tgccgtttgc ggccatcacg ctgcaggaaa aaaacaggcc gccgaatatt
    15361 attgctgtat tattttttct catcgtctgt ttccccgaaa ttatccagcc aggtcacccc
    15421 gttttcccgc ttcagaataa ggatgtaatg tttgatatca gtaaaaggtt cggagttacc
    15481 aatccatgtt ttccgaattc cacggatttc cacacgacca tattgtggcc agacacggac
    15541 actggtctgg aaaaaagcag aattcacgtt atctttttta ataagtttcg cttcttcagc
    15601 cagaataact ttcatctgat tctgtgcgcc cggacggata tattgcagaa gcgcctgatg
    15661 tgaggcatcg acgatttccg gtgaaacatt cagacgaagg gcaataaatg ccagtgccat
    15721 ctgctgtaaa taagaggcat cggcactgtt ctgtgacacg gcaaagggta cattaaatcc
    15781 catcgggatc acggcagtgc gttgctcatt ctgaagacgg tagttattta tcccctgaat
    15841 gacgttaacg gcgaggctga ggacaataag caccgacata aatataaagg cgatggccat
    15901 tacacgactg gtacttaaac gggcaccgtg ttccatagaa taatcctggt atcagttcta
    15961 tttaatccac tgccggaaac acgaatcggg aacattatga aaaataccgc gcaacagggc
    16021 tgttggcata taccagtaaa tcaggtcacg taaccaggag ctgccccgcc cttttttcag
    16081 ttttttaatc ccgaaataaa ccagaaccgc tgcaccaata ccaaacagat attttgatgt
    16141 catcattccc cagccaatac aaattccggc aggaataagt tcatccagcg gcaggccaaa
    16201 ccagcggctc tggttggtca gtgtttccgg gaaacgatat tttttgagtt tattctcgtc
    16261 tcccgacatg acgttatttc ctgtcagaga ccgacgactg ccatacccac ggtaataaac
    16321 acggagataa tggcaaaacc ggcgaggaac ttaacgtttt tggtcatcat gtacatgatg
    16381 gccccgacga gaacttccgc cagaatcacc catttcaccg cactggagtc tttaccgaag
    16441 gtggccttga cggtggcgtt acccccggcc atcaggtcct gacctgcccc ctgggcggcc
    16501 agtgccagtt gcgggaagaa catcatcaga acagcagccg ggatcactgc acgaaccagg
    16561 cgaagcatat tcagacgcgt gaatttagga aaaaacgact tctttttgac gggcgcagaa
    16621 gcaccctgaa cactcaaaat agcattcata agttacctct attgtggata gattaataaa
    16681 cttaaagatc tttaattatc gaatcagttt cccctgttcc ctcctttatt gcatccagat
    16741 tataaaagtc aggaaaacgg ctcagatgat cacgtaacct gactagcacc tcattagttt
    16801 ttgttctccc gctacgctcc cgggcaccta agagcttgtg attagtagca tcgtccaata
    16861 ccagtaaaac tttattgccc ggagcaggcc ttattatatt tctgctcaag tctccccctc
    16921 agccatttag ataattaaat ataattatac gaatccataa aaatagaaac aaaaataacg
    16981 taaattatat taacaaaaaa gggaaccgat ggttcccttg agttcacaat aaatta
//
LOCUS       DAZGSP010000109        17006 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZGSP010000109 DAZGSP010000000
VERSION     DAZGSP010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.110"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(20..172)
                     /locus_tag="ACHK74_004024"
     CDS             complement(20..172)
                     /locus_tag="ACHK74_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0222758.1"
                     /translation="MPQRTFLTMLIVVCVTILCFVWMVRDSLCGFRVEQGNTVLVATL
                     AYEVKR"
     gene            505..747
                     /locus_tag="ACHK74_004025"
     CDS             505..747
                     /locus_tag="ACHK74_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222759.1"
                     /translation="MSNEYQSTSINPDVFEQFFTLLNTHCKSSEAALAMLSTLCYELM
                     RRSGIHSLDSLMLNGEGILIQLQGADIREKTGQHIH"
     gene            complement(840..2375)
                     /locus_tag="ACHK74_004026"
     CDS             complement(840..2375)
                     /locus_tag="ACHK74_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP0222760.1"
                     /translation="MSCIKEKSKYAFERAKQFYFMLWCKESLIYLLFSVLVTWCCGYT
                     VKNTLGNVLILFLMLLLGRQNVISATCVCIFLLLSAFYAPVGMIYGKINNSFIASVMQ
                     TNLNEAGEFITGIPVLYFIVSLIIAAFTFWFWRKRDKSHRGFIFLLFFIVLSVNAWPK
                     RMVTNVMTGIAETREEMMRYESLKHNQKDSWDIVNVEKKYKTIIIVIGESVRRDYLSV
                     YGYPLPTTPWLNSAPGIFINGYFSAAPNTIGSLSRTLTLDYTETGNPGNNIVTLARKA
                     GYETWWISNQGSLGRHDTLISVIAANADKKYFLKEGSSSSRRIDDEDMLQYVREAVNS
                     SSPKVIFIHMLGSHPNPCDRLFNDAQPFRELFGNKVSCYLSTLLKLDRFLQAIYNMTT
                     EHGDDFAMLYFSDHGQSVSSDSTPVHHAPGLRSGYDVPLMMMASDITEHHLNHVHISA
                     RRFPEIFQWITGIQTKNLPLSFSLLQNDFAESVSVFNGERDVPVMSLPEEPILTGNAE
                     SEK"
     gene            complement(2575..3030)
                     /locus_tag="ACHK74_004027"
     CDS             complement(2575..3030)
                     /locus_tag="ACHK74_004027"
                     /inference="COORDINATES: protein motif:HMM:NF022214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HFP0222761.1"
                     /translation="MRDKSEVLTGAQKNRFGLILYIILLSVVYILITRLNLTTTYYSL
                     YSSLDENSQVTEYFIISIATFLFLAALCLFFIYSFFKRKRVVGKLLLCIYILYISHYA
                     ITFVYCLYVVGAECMTTGKIEHILIDIIIAMLSLSYVCLSGRARHIFVN"
     gene            complement(3118..3741)
                     /locus_tag="ACHK74_004028"
     CDS             complement(3118..3741)
                     /locus_tag="ACHK74_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0222762.1"
                     /translation="MQERIDYHIEKYQFRARNESPRLMRQWADVMRECRETGAGARER
                     LRTALLNVDYVTSFELPFRLLLTRTPQMIDTLRREWLLSQKKALFNGRQRGCVYSLQN
                     DLSGVPDCFSYSLATRIRRMDCYGMTTRCFTDIAGQVKAPTARLKLALESGLLVTALD
                     GLFWLGTQRIAADVQRLRQSGMVIVTREVEVSDSLTGTTRLVSAYTL"
     gene            3890..4263
                     /locus_tag="ACHK74_004029"
                     /pseudo
     CDS             3890..4263
                     /locus_tag="ACHK74_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            4907..5272
                     /locus_tag="ACHK74_004030"
     CDS             4907..5272
                     /locus_tag="ACHK74_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222763.1"
                     /translation="MNDGRQTPPSPEAWKIRFLANGTPGTWFYTDDAAVTKEWLRAHC
                     DDVEVIPLCRMHDGPVFREHHINELSAALVRMLHMHHILLERTRYRENIHDAECIREI
                     HEAPLQAIGILEKYKRTKS"
     gene            5282..5473
                     /locus_tag="ACHK74_004031"
     CDS             5282..5473
                     /locus_tag="ACHK74_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222764.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQEFRTEKACRDAFTAL
                     KKENVGEFALRGVCVPAGE"
     gene            5507..5830
                     /locus_tag="ACHK74_004032"
     CDS             5507..5830
                     /locus_tag="ACHK74_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFP0222765.1"
                     /translation="MAYHPPDITLHYPYLRRMSWAQITAAAEDAEIHHDYARALILWQ
                     HAYHAATLTLNKNLAVAKIDFCIKRIRMREQMSGIIRNTGTDEILFRLSKKHHLYEKK
                     KTKEG"
     gene            complement(5976..6071)
                     /gene="dqlB"
                     /locus_tag="ACHK74_004033"
     CDS             complement(5976..6071)
                     /gene="dqlB"
                     /locus_tag="ACHK74_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFP0222766.1"
                     /translation="MINLLIIVLRAVVAVVNAVIALLELIRELID"
     gene            complement(6722..6817)
                     /gene="dqlB"
                     /locus_tag="ACHK74_004034"
     CDS             complement(6722..6817)
                     /gene="dqlB"
                     /locus_tag="ACHK74_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFP0222767.1"
                     /translation="MINLLIIVLRAVVAVVNAVIAILELIRELID"
     gene            7362..8633
                     /gene="trbA"
                     /locus_tag="ACHK74_004035"
     CDS             7362..8633
                     /gene="trbA"
                     /locus_tag="ACHK74_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HFP0222768.1"
                     /translation="MSYQQTSATEDPMAIWYIVGAICLLFAIIIWRFLPEIVFASCLI
                     LHTLWGMIDWGPFHNFAAPRYNLLAITANNAATITFSQWLDVMSRTVGILWLILLPMT
                     FGFLWMWFHHPAQPRFTRRPLNIHTLPHIFSALSPAIAPVLADGDNNRLFHGQKRPER
                     RVALTPEAFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQKWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEACRLGLPCPEPCVEEAIEGLRQDMLRLGFIWDEPQPDRDRRRQIRTRWSLTDDVI
                     PRRHDNDEGSDTGETTETTETRHPADKEKAQ"
     gene            8630..9748
                     /locus_tag="ACHK74_004036"
     CDS             8630..9748
                     /locus_tag="ACHK74_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFP0222769.1"
                     /translation="MTIRYTATLNNKHLSLTALYPDGGQEVIAILPVTELGALINRNN
                     RLMHLSFSTPFREITETLLVDTVDTERPGHHIGRMLSAIDSCLSAAYAELPARKTSFM
                     RGLIYGAGLVLALLTAVTAGYILLKPDSDSPLRVPVTESVTETAPLHIDKEIVRSVAP
                     SETKNEIVHPAPQEMQETSATPAITADKNTLVAEARRNLATVLKRNADRGMFTINLST
                     GHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGEASTNQTAPLLCEPD
                     AQKRATGWHQLYSADGGMAPTGDAPAAPDEKCLTAARAAIDVNNQAFRQFGFGGTPWV
                     LSDTGWHLPSGILKDATTVSLFLKATDTEDNHNEPASR"
     gene            9729..12029
                     /gene="trbC"
                     /locus_tag="ACHK74_004037"
     CDS             9729..12029
                     /gene="trbC"
                     /locus_tag="ACHK74_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFP0222770.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNIIGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFTLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHIIFFSTTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVRERK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFITQQSMILARDLGYRLEGTDAQTLEVKKYKGRFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMMAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTEKTSRKRRKKHTEPYRIIDTFQDQLKRTQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKEILNTLLPLEILLPVAE
                     KNKSQTINNGHPQKSYGAQPTTDEKY"
     gene            complement(12490..15120)
                     /locus_tag="ACHK74_004038"
     CDS             complement(12490..15120)
                     /locus_tag="ACHK74_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP0222771.1"
                     /translation="MAKQAESAGSEKLLYNRLKQFDEPGLFHDNYQTPDYVQANLKDD
                     LRPYQHAALRYLHYTQRNPEEALQHYRHLLFHMATGAGKTMVMAGAILYLFKEYSYQN
                     FIFFVHTDAIIQKTRENLLNPQSPKYLFSQNLEIDGEKITIIPVDIFPQVPERNTIYL
                     RLSTIHKMHDELSSYRENGITSEDLKELPLVLLGDEAHHFNAGTKARGKAKNTSENEE
                     HTWERTIENILDLRPDNRLFEFTATINLANKDIWQKYRDKVVYQYDLKKFMSDGYSKK
                     VMLLEANQNDDDKMLDAVLLSQYRKLTAEDHGITDFKPVLLFKSNKIAVSKAKQAEFS
                     QLIAGLTPENVRRHLSNKKVRLSSDTSIWHKVIQRYDDSDLITVIGQIQEDFNDFNLL
                     NVNRSDLLEENPVLLNTLEDVDNPVRAVFAVAKINEGWDVLNLYDIVRISEQASSSKT
                     GTDSEAQLIGRGARYYPFVYDREKSFTRRFDNSTRDLSILEQLHYHTINEPAYIKMLH
                     ASLEQADIDAQHDGSGTIEHARLKEDFKKSAVYRTGKLFFNEVEDIESSSRTWETYSL
                     ETRFDIPYQTASEASLDNLAGTECVTKAVPLVLDERFYRKAMQRISFYSLDNLQRFFP
                     MLTSVRDFIRCEEYLGRLRITVTIPLSLDFSAIPAKEKLRLLETVLLRIADNVRRNDK
                     KVKGSYRFISQPVREVIKDYSLYIDQSVIMNQKITAAQTIGKKWYVYDNAILNQLEHR
                     LVKTLEKVMPKLRERYDDIYVLRNDEQSTRFKLTEFGGVRGFMPDFIMILICKDDNSY
                     WQVFLEPKGDDRLQVDEWKENLLRSLNNDKRLILEEDASVRLIGIKFFADSKMDEFIK
                     DLEEKLNDGLPLEERSLSLF"
     gene            complement(15125..>17006)
                     /locus_tag="ACHK74_004039"
     CDS             complement(15125..>17006)
                     /locus_tag="ACHK74_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFP0222772.1"
                     /translation="ELIRELNSVLRGFPLFWNGQELQRSVVIDAINRKQPEIIKALVA
                     NEKIKSVYGTEIDGVLIFEFERLCSLLKYKEYWANSYTRYRNKIGLTSEGKYLDYNSD
                     VVLDFPFKDCVLEGGMTKEEQGKDEIYYNEIIARDEIDRLFSNKVFTNIKRYTKYGVE
                     ENITSFESDNLIIKGNNLIALHSLKQRYAGKVKLIYIDPPYNTGTDSFKYNDSFNHST
                     WLSFMKNRVEIAKELLSEDGSIWINLDDNEAHYLKVLMDQDTGLGFVADVIWQKRTSP
                     DARVPLGAGHDHILVYSLNPELFRAKINLIPLTQEQKNRYSNPDNDPNGPWVSSDFTA
                     QGFRPNQMYEIKTPSGKSYYPPEGTCWKNIESEMIRQKEEGKMWFGVNGDAMPRRKTY
                     LKDVKGRMPWTWWPNSEVGHNQEAKKEINALNMDSVFSTPKPERLIQRILHIGSNDGD
                     LVMDFHLGSGTTAAVAHKMGRRYIGIEQMDYIHNITVPRMQKVIDGEQGGISKDVGWQ
                     CGGSFLYVELMELNALFVNEIRKAQSSEELEKLFAVMKTEAHLNYQVALENVLSAEYE
                     VDGIFRKVAFCELELHEQKKLLIEILDKNQLYVNASDMDDSDLNISESDKAFTRSFYG
                     ME"
BASE COUNT         4677 a         4369 c         3620 g         4340 t
ORIGIN      
        1 cctgccgccg tgttgcccgt taacgtttaa cttcgtaggc taacgttgcc acaagcactg
       61 tgtttccctg ctcgacacgg aatccgcaaa gcgaatccct caccatccag acaaaacaca
      121 gtatcgtcac acagacgaca attaacattg ttaaaaacgt tcgttgtggc atcgcagccc
      181 ctccattgcc tttcggccgt catcgggcta ctataatgtt gtctaggcat tacagtgggc
      241 cctgggttga ttgataaaaa aatcgcctgg ggctttctgc tgcatgccgt ccacaaaacg
      301 ctttcagcat acagcctcaa gcgcccgccg ctattctaat cgattttcag tctgccacaa
      361 tcgttttcca cctaatgaca tgtaatgcac aacagtattt ttacgggaaa ctacacggca
      421 aggattcccg gaacacgttc attattttat ggttacagac atcatgttaa atccggaagt
      481 aataatgacc tggaaggaaa tactatgagt aacgaatatc aaagcaccag tataaacccg
      541 gatgtttttg aacaattttt taccctcctg aatacacact gtaaatcatc agaagctgca
      601 cttgccatgc tgagtacact ctgctacgaa ctgatgagga ggagtgggat ccattcactg
      661 gacagcctga tgctcaacgg agagggtata ttgatacaat tacagggggc tgatattcgc
      721 gaaaaaacag gtcagcatat tcactgatcc aggtatgggc tgaaaacagt gtcagcctga
      781 aataaacgct caatgcctca tacacatgag gcattgagct cggtcataaa taatctttat
      841 tatttttctg attcagcatt tccggtcagt atcggctctt ctggcaaaga cattaccgga
      901 acgtcacgtt caccattgaa tacagaaaca ctttcagcga aatcattctg taacaaagaa
      961 aaacttaacg gcagattttt ggtttgtatc cctgtgatcc attgaaaaat ctcgggaaaa
     1021 cgacgtgcac tgatatgcac atgattgagg tggtgctcag taatatcact ggccatcatc
     1081 atgagaggta catcgtatcc cgaacgcaaa cctggcgcat gatgcacagg ggtgctgtct
     1141 gaactgacgg attgcccatg atcagaaaaa tacagcatgg caaaatcatc accatgctct
     1201 gtcgtcatgt tataaattgc ctgaagaaac cgatccagct taagcaatgt tgataaataa
     1261 caggatactt tatttccgaa taattccctg aacggctgtg catcattaaa aagtctgtca
     1321 cagggattag gatgggaccc cagcatatga atgaaaatca cttttggaga agacgaatta
     1381 acagcttccc tgacatactg aagcatatct tcatcatcaa tgcgacggga agaagagctc
     1441 ccttccttaa gaaaatattt cttatccgca ttggcggcga tgacagaaat cagcgtatca
     1501 tgtcgcccta aagagccctg atttgatatc caccacgtct catatccggc ttttctggca
     1561 agcgtaacaa tattattacc cggattacct gtttcagtgt aatcaagcgt cagtgttctg
     1621 ctgagtgaac ctatcgtatt cggagctgcg gagaaatacc cattaataaa tatacccgga
     1681 gcagaattta accatggtgt tgtcggtaaa ggatacccat aaacagaaag ataatcccgt
     1741 ctgacacttt caccaattac gataattatg gttttatatt tcttctccac attcacaata
     1801 tcccatgagt ctttctgatt gtgtttcagc gattcatatc gcatcatttc ttctctggtt
     1861 tcagctatcc ctgtcattac attcgtaacc atacgttttg gccatgcatt tactgacaat
     1921 acgataaaga acaacagaaa gataaatccc cggtgacttt tatcacgctt acgccagaac
     1981 cagaatgtaa aagcagcaat gatcagtgaa acaataaaat aaagaacagg tatgcctgtg
     2041 ataaattcac ctgcctcatt tagatttgtc tgcattacag acgcaataaa actgttgttt
     2101 atttttccgt aaatcatccc aaccggagca tagaatgcac ttaacaaaag aaagatacag
     2161 acacacgttg ctgaaataac attctgccgc cccaacaaca acatcaggaa caatataagc
     2221 acattcccca gggtgttttt cactgtatat ccgcaacacc atgtaacaag cacagaaaac
     2281 aacagataaa tcagtgactc tttacaccac aacataaaat aaaactgttt tgctctctca
     2341 aaagcatatt ttgatttttc tttaatacaa gacacgccag tcattttcct gtacaaacac
     2401 agacgatcat ttcaacacat ggatatttat atgaaacagt tcacaaaaat accctaccct
     2461 gtccagcagg gtttacatat tatcaggttc tgtgtttaca gcaactgctc cccgaaccag
     2521 attattcgca taacccgatg aaggcctcat aaccataaaa cactcagaat acatttagtt
     2581 aacaaaaata tgtcttgccc tcccggaaag gcaaacatac gaaagagaaa gcatggctat
     2641 aataatatca atcaaaatat gctcaatctt tccggttgtc atacactcag caccgacaac
     2701 ataaagacaa taaacaaaag tgatagcata gtgtgatata taaagaatat aaatgcacag
     2761 aagtaatttc ccgacaactc ttttccgttt aaagaaagaa taaatgaaga acaaacataa
     2821 agccgccaga aaaagaaagg tagctatgga tattataaaa tactccgtca cctgtgaatt
     2881 ctcatccaga gatgaataca gcgaataata tgtggttgtg agattaagtc ttgttatcaa
     2941 tatataaaca actgataata gtataatata aagaatcaga ccaaatctgt ttttctgcgc
     3001 tccggttaat acttctgatt tatctcgcat aaaatatcct gtctgtaatc tgtcccggta
     3061 aacaatggta aatttcagaa tcagtgcgaa tacggtaaca aagattcctc ctgtgcttta
     3121 aagtgtgtag gcagaaacca gtctggtggt tccggtcagg gagtctgata cctcaacttc
     3181 ccgggtgact ataaccatac ctgactgacg cagtctctgc acatcagctg caattcgctg
     3241 agttcccagc cagaacaggc catccagtgc cgttaccagc aacccgcttt ccagcgccag
     3301 tttcagccgt gccgtcgggg ctttaacctg cccggcgata tcagtaaaac aacgcgtcgt
     3361 catgccatag cagtccatcc gccgtattcg cgtcgcaaga ctgtagctga aacaatccgg
     3421 cacgccggat aagtcattct gcagactgta tacacatccc cgctgccgcc cgttgaacag
     3481 ggcttttttc tgactgagca gccattcccg acgcagagtg tcaatcatct gcggtgtccg
     3541 ggttaacagc agacgaaacg gcagctcaaa actggtcaca taatcaacat tcagtaatgc
     3601 tgtccgcagt cgctccctgg cacctgcccc ggtctcccgg cattcacgca tgacatcagc
     3661 ccactgacgc atcagtcgtg gagactcatt cctggccctg aactgatatt tctcaatatg
     3721 ataatcaata cgttcctgca tatcgccacc ggatatctgt tgtttaccca gacacagtga
     3781 ttagcctaca ggaataaata tcctccgtga agccaggtgc ctcagaagat aaaaacatgt
     3841 acttttttca acgtgtaata ttaacaccac caccacagga aaatataaaa tgataaaata
     3901 tatattcgca ctcacacttc tgctttccgg gtctgtctgt gccgatcaaa ttcagggact
     3961 cgttattcgt gtgctggacg gagatttgtg gtaaatacgc ccgtaaccgt gacatacaca
     4021 cagacagacc gctacgggcg tattcttgga cgggtatata ccgaagacgg catggaagtt
     4081 aaccgctggc tggtacagca tggtgcagcc tgggtatatc ctgatttcaa cacagattac
     4141 accctgccgg tataccagag agaagccaga acaatgaaac ggggtctgtg ggcagacaaa
     4201 aaacctgttc caccatggct gtggcgaaaa gaaaataaaa atagccacaa taattataaa
     4261 taatacatct cacagaaata tatttcctgc cgttttattt cccctgccag tccccaacgg
     4321 gaaataacct ccagaaatta cacagatgtg aataataaac ctgttcgatg tatccgtcac
     4381 aaaagagttg atgtttgact cagaagaaaa tacgcctgaa gttaaggaag gcttcagctt
     4441 catgattgtt ctgacatgaa catatatccc tctgtagcgc cctgcctttc ataacatttc
     4501 tgttacagca acaacgaaag acaacagcaa tgtcggcctt ttacgtccac atgcatattc
     4561 cacttatccg gaattgtgac acagcccctg aatacaggat tgattgccag tgcccacaaa
     4621 tggcacattt tcagtaagat tctgaatgtt acattccccc gtcatgtgtg gcacaactgt
     4681 caccgggaaa gaaacggaaa gcacatgaaa aacatcaaaa ctcacaccgg actccttatc
     4741 cacaaagaac aaacgcggcg cgttcgtctt catgaaactc cggccgcctg gtgccacacg
     4801 caccgggaat gctacagcaa aacgaccggt cgtcgctgcg gcagccctga gagtctcagc
     4861 aggctgattc tgagcagcat tcagcccata gaatgagatg acccgtatga atgatggcag
     4921 acagacgcct ccgtcccctg aggcatggaa aatccggttt ctggcgaacg gtacacccgg
     4981 cacatggttt tacacggatg acgcagccgt cacaaaagag tggctgcggg cacattgtga
     5041 cgatgtggaa gtgatccccc tgtgccgtat gcatgacggt cccgttttcc gggaacacca
     5101 tattaatgaa ctcagcgccg cactggtcag aatgctccat atgcaccaca tactgctgga
     5161 acgaacccgg tacagagaaa atatccatga tgcagaatgt attcgtgaaa ttcacgaagc
     5221 tcccctgcag gctatcggca tcctggaaaa atacaaacgc acaaaatcat aaggcagttc
     5281 tatgtggata ctgattttct ggctctcatc cccctttaat gtctcctccg gcgatgcccg
     5341 gcaacccaca ctggccgttc acagtcagga gttccgtact gaaaaagcct gtcgggatgc
     5401 cttcaccgcg ctgaaaaaag aaaacgtggg agaattcgct ctccggggag tctgcgtccc
     5461 cgccggagaa taaagcggag atgatgatta gccacaggag tattcaatgg cataccatcc
     5521 cccggatatc acactgcatt atccgtatct gcgccggatg tcctgggcgc agataacggc
     5581 agcagctgaa gatgcggaaa tacatcatga ttatgcccgg gcattaatat tgtggcagca
     5641 tgcatatcat gctgccacgc tgaccctcaa caaaaacctg gcagttgcta aaatcgactt
     5701 ctgtattaaa cgtatccgga tgcgtgaaca aatgagcggg ataatccgga atactggcac
     5761 agacgaaata ctcttccgtc tgagtaaaaa gcatcattta tatgagaaaa agaaaacaaa
     5821 agagggatga cagtcccatt gtatgggaaa aacttgccat gcttactgca ggtgcatttt
     5881 tgatactggc agtcctgggt gagtaatctt aatatcctgt ttattaaagg gcagaaggcg
     5941 gtccccctct gcccttaagt acgtttccgg gattatcagt caataagttc ccggatcagt
     6001 tccagaagag ctatcactgc attgaccaca gcaacaaccg ccctgagaac gatgattaac
     6061 aggttaatca tccgtttctc catcaatgga ggaagtcacc gttgcttgct catacatcgc
     6121 ctgtcacaac ggctctctga cattgttgga tgtaaagtac cttcccccct gccagagcac
     6181 tgaacacagc accacctgtt tgttcagcca ggactttaat tcatccatac acagatagaa
     6241 caggttttag aagcaaggga ggcactctgt acagcgccaa cacaaatatt atacagatgc
     6301 acacataaaa ttcatttaaa gaaatgaata ttgacaggca atctgaagtc aaactagaca
     6361 acagatgtct ggtgccataa tattatggat gttggatgta gagtatcttc ctccggtcag
     6421 agcaccaact ccgctccgga atcacaaaaa accggcctca ccagtcggtt tttttacgtc
     6481 tgcacctccc ctcctccccg gacaactgct ctctcactcc cgttgaatat ttcctgttga
     6541 tttttacgaa aatacggcaa atgggggata tcgaatatat ttcctggagt gtagtcggta
     6601 aaaaccagct catgtttgac tcacagaaac aggctctgga agtacagaaa gtaatctgtc
     6661 cgtagtttat taaagggcag agggcggtta cccgctgccc ttaagtacgt ttccgggatt
     6721 atcagtcaat aagctcccgg atcagttcca ggattgctat cactgcattg accacagcaa
     6781 caactgccct gagaacgatg attaacaggt taatcatccg tttctccgtc aatggaggaa
     6841 atcactgctg cttactctta cattgcctgt cacaacagct ctctcattgt tagctgaaaa
     6901 gagacttcct ccggccagag caccagacac agcaccacct gtttgtccgg ccaggtcttt
     6961 gaatagcaca gatccatagt cccgatggat tgatgcgaag gtaagtctcc agacaaggct
     7021 aacttactga ttatacttgt ttcaggcgat atcgctactc ccggaataat agattgtaac
     7081 tgacagattg cgatcaggtg aatataacgc tattctggta acgttagttg aaaagagact
     7141 tcctccggtc agagccacca actccacccc ggaatcgcaa aaaaaccggc ctcaccagtc
     7201 ggtttttttg tacctgtctc ccaccacctt ctgccgttcg ctcttctctg tgaaaatgtc
     7261 ctgttgaact cattcctgtc cggttattac cggcgctgat tcgcattcat gccacccgac
     7321 ttaatcccga aatttatcca ttcatctatg gaggaatccc cgtgtcttac caacagacgt
     7381 ctgccaccga agatccaatg gctatatggt atatcgtcgg tgccatatgc ctgctctttg
     7441 cgattatcat ctggcgcttt ttacctgaaa tcgtcttcgc ctcctgcctg atcctgcaca
     7501 cactgtgggg catgattgac tgggggccct ttcacaactt tgccgccccc cgttacaacc
     7561 tgctggccat cactgctaac aacgcggcaa ccatcacttt cagccagtgg ctggatgtta
     7621 tgtcccggac agtggggatc ctctggttga ttctgcttcc catgacattc ggctttctgt
     7681 ggatgtggtt tcaccatccg gcccagcccc gttttacccg caggccactg aatatccaca
     7741 cactgcccca tattttttca gctctgtcac cggcgatcgc ccccgttctg gctgacggag
     7801 acaacaacag actcttccac ggacaaaaac gtcccgagcg ccgtgtcgca ctaacccccg
     7861 aagcctttgt ggagcagaac aaccttatcc gcaacatgca gctcgatgtt gcgtccacac
     7921 gccagtgctt catggcgcaa ctggggcaac cactgaccag ctggaaagac atggccccac
     7981 atgaaaaagc gctctttgcc atctttggcc tgcagttctt cctcggtgac cggaaagcag
     8041 cggtagccct gatgaacaac ctcaatctct cctgcaggct caaaagtaaa cgggaccagg
     8101 ggcgtttcag cacgccggtg tattcactgg ccagaaacgc ctttatccgg gtcatcaaaa
     8161 cagaaggcgc acaaaagtgg ctcaggcagc accgttacgt tcgcagcggt ctggtctggc
     8221 tttacgcaca tgatttgcgc ctgacgcctc cgaactggct gtggctcaag ggcgttgacc
     8281 ggacgctctt ctatgccctt caccgggcaa acaccacaaa aggcttcatc gaaggggccg
     8341 gcgtggtagc cgttgcccgg gcagaaaatg aagcgtgtcg tctcggcctg ccctgcccgg
     8401 aaccctgtgt ggaagaagcc attgaagggc tgcggcagga catgctgaga ctggggttta
     8461 tatgggacga acctcagcca gaccgcgatc gcagacgtca gataaggacc cgctggtcac
     8521 tcacggatga cgtgatacca cgccgccatg ataacgatga aggctcagat actggtgaaa
     8581 ccaccgaaac cactgaaacc cggcatcccg ccgacaagga aaaagcacaa tgaccatccg
     8641 ctacactgca acactgaaca ataaacacct gtccctgacc gccctgtacc ctgacggtgg
     8701 tcaggaggtt attgccatcc tgccggttac agaactgggc gcactgatta accgtaataa
     8761 caggctgatg cacctgtcct tcagcacccc ttttcgtgaa ataaccgaaa cactgcttgt
     8821 tgataccgtt gatactgaac gtcccggaca tcacatcggc agaatgctct ccgcgattga
     8881 cagctgtctg agcgcggcat atgcagaact tcctgcccgg aaaaccagct tcatgagagg
     8941 gctgatatac ggtgcaggat tagtgctggc acttctgaca gcggtaacag ccggatatat
     9001 tttactgaaa ccggacagcg attcccctct cagggtacct gtcacagaat ccgtaacaga
     9061 aacagcacca ttacacatcg acaaggaaat tgtccggtca gtagccccgt cagagacgaa
     9121 aaatgaaatc gttcacccgg caccacagga aatgcaggag acatccgcta ccccggcgat
     9181 aacagcagac aaaaatactc tggtcgctga agcccgccgt aatctggcca ctgtacttaa
     9241 acgcaacgcg gaccggggta tgttcaccat taatctctcc actggtcatg aacgcacact
     9301 ctacgccttc cttgaccccg catgcatcaa ctgccgtctt ctggagcccg cgcttaagca
     9361 gctggcccgg gaattcagcg tggtgattta tccggtctcc gtaatcggcg gagaggccag
     9421 cacaaaccag actgcgccac tgctgtgtga accggacgca caaaaacggg cgaccggatg
     9481 gcatcagctg tattccgcag acggtggtat ggcacccacc ggcgatgcac cggctgcacc
     9541 ggatgaaaag tgtctgaccg ctgctcgtgc tgccatcgat gtcaataacc aggctttccg
     9601 ccagttcggt tttggcggta ccccatgggt gttatccgat accggctggc acctgccgtc
     9661 cggcatcctg aaagacgcca ctaccgtcag cctgttcctg aaagccacag ataccgagga
     9721 taatcacaat gagccagcat cacgttaatc ctgacctcat acaccgcacg gcctggggaa
     9781 acccgctgtg gaatgcactg cacaacctga acatcattgg tctgtgtctg gccggcagta
     9841 tcattacggc cctgatatgg ccactggccc ttcctgtctg tctgctgttc accctggtca
     9901 cctccgtgat attcacgctg caacgctggc gatgccccct gcgtatgccc atgacgctca
     9961 gccttgatga cccctcccag gacagaaagg tcaggcgaag cctcttcagc ttctggccca
    10021 ccctgtttca gtatgaagca gatgaaacat cccccgcacg ggggattttt tatgtcggtt
    10081 accgccgtat caacgatatc ggtcgcgaac tctggctgag catggatgac ctcacccggc
    10141 atatcatatt tttttccacc accggtggcg gtaaaacaga aaccaccttt gcctggctgc
    10201 tcaaccctct ctgctgggga agaggcttta cctttgttga cggaaaggca cagaacgata
    10261 ccacgaggac aatctggtat ctctcccgac gttttggccg ggaggatgat atcgaggtca
    10321 tcaacttcat gaacggcgga aaatcccgca gtgaaatcat tcagagcggc gaaaaatccc
    10381 gcccacagtc aaacacctgg aacccgttcg cattcagcac cgaagccttc accgccgaaa
    10441 ccatgcagtc catgctaccc cagaacgttc aggggggaga atggcagtca cgggcgatag
    10501 ccatgaacaa agccctggtc tttggcacaa aattctggtg cgtccgggaa agaaaaacca
    10561 tgtccctgca gatgttacgg gagcatatga cgctggaagg gatggcaaaa ctctattgcc
    10621 gtgggcttga tgaccagtgg ccggaagaag ccatcgcacc actgagaaat tacctgcagg
    10681 acgttccggg attcgatatg tcactggtga gaacgccgtc agcctggact gaggagcccc
    10741 gtaaacagca tgcttatctc tccggtcagt tctcggaaac attcacaacc tttgccgaaa
    10801 ccttcgggga cgtctttgcg gcagatgccg gcgacattga tattcgcgac agtatccaca
    10861 gcgaccgaat tctgattgtg atgatccccg cactggacac atcagcccat accacgtcgg
    10921 cactcggtcg tatgttcatc acccagcaga gcatgatcct cgcccgtgac ctgggctacc
    10981 gcctggaagg cacagacgca cagaccctgg aagtaaaaaa atacaaaggc cgcttcccct
    11041 atatctgtat ccttgacgaa gtcggtgcct actacacgga ccgcattgct gtggaggcca
    11101 cccaggtacg ttcactggag ttttccctga taatgatggc gcaggatcag gagcgaattg
    11161 aagggcagac ctccgccacc aacaccgcca cactgatgca gaacgccggc accaaattcg
    11221 ccggacgcat tgtcagtgac gacaaaacgg caagaacggt gaaaaatgcc gccggtgaag
    11281 aggcacgggc cagaatgggc agcctgcagc gccacgatgg tgtgatgggt gagtcctggg
    11341 tggacggtaa ccagatcaca atccagatgg aaagcaaaat cgacgtgcag gatttgatca
    11401 gactgaatgc cggtgagttc tttaccgttt tccaggggga tgtggtgccg tcagcctctg
    11461 tgtatattcc ggacagcgag aaaagttgcg acagtgatcc ggtggtcatc aaccgctata
    11521 tttccgttga agccccccgg ctggagcgtc tccgccgtct cgtccccagg acagtgcagc
    11581 gcaggctccc cacaccggag catgtcagca gcattatcgg tgtgctgaca gaaaaaacct
    11641 cccggaaacg tcgtaaaaaa cacacagagc cttaccgaat catcgatacc ttccaggacc
    11701 agcttaaaag aacacaaaca tcgctggatc tgcttccgca atatgataca gatatcgaat
    11761 cccgggcaaa cgaactgtgg aaaaaggctg tgcacactat caataacaca accagggaag
    11821 aacgccgggt atgttatatc acactgaatc gtccggaaga ggaacactct ggtcctgaag
    11881 atataccttc agtgaaggag attctgaata ccctgctccc cctggagata ctgttaccgg
    11941 tagctgaaaa aaataaatcg cagacaataa ataacgggca tccccagaaa tcatatggag
    12001 cacaaccaac aactgacgaa aaatactgaa ggaaaacata tattttctgg tgaccatatc
    12061 tgtcaatcat actgtatatg tccggggttt tacggcacac gggataaggg ccgcagacgc
    12121 ggcccttaat tcagggctaa ccgggcataa acatatggac acgcatgtaa atgatgagtt
    12181 aaaaaataat ccgtgcgttt ccggtgatga tattagttgt tggtaacgaa atctcattag
    12241 cgggccggaa acgcacttct ccctttcagc cggagtaacc ttcaaatatc actggcggag
    12301 accagctagt tgaattcacc tcacaacctt gcattagccg aatcccctgt cagttcgttg
    12361 taacacgcca gtggcatcat tttcacacta tattatccgt gacaatatcc cgataaacat
    12421 cccaccacat gcggatacgg tttgtattca gtaaatacca gaaacaactg ttttcctcca
    12481 ctcacctggt cagaacaatg acagagaacg ctcttccaga ggaagaccat cattcagttt
    12541 ttcctctaaa tctttaataa actcatccat tttgctgtct gcaaaaaact ttatgccaat
    12601 tagcctcaca ctggcatctt cttcaagaat cagtcgttta tcattattca gagaccgaag
    12661 cagattttct ttccattcat caacctgcag acgatcatct cctttaggct ccagaaagac
    12721 ctgccagtac gaattatcat ctttacagat cagtatcatg atgaaatcag gcataaagcc
    12781 acgtacacca ccaaactcag taagtttaaa gcgcgttgac tgttcatcgt tacgcagaac
    12841 ataaatatca tcataacgtt ccctgagctt cggcatgacc ttttccagcg ttttcaccag
    12901 acgatgttca agctgattca gtattgcatt atcgtataca taccattttt tcccgatagt
    12961 ctgagctgcc gtaattttct gattcattat cacggactgg tcaatataca gactgtagtc
    13021 cttgataacc tccctcactg gctgactgat gaagcgatac gagcctttca ccttcttgtc
    13081 attacgtctc acgttatctg ctatacgcag cagaacggtt tccagcaggc gaagtttttc
    13141 ttttgcaggt atggcagaga aatccagcga tagcggaatt gtgactgtga ttctcagcct
    13201 gcccagatac tcttcacaac gaatgaagtc acggacagag gtcagcatcg gaaaaaaacg
    13261 ctgcagatta tcaagtgaat aaaagctaat cctctgcatc gccttgcggt aaaagcgctc
    13321 atccagtacc agcggtactg cttttgtcac acattcagtt ccagccaggt tatccagtga
    13381 tgcctcactg gctgtctgat acggtatatc aaaacgagtt tccagcgagt atgtttccca
    13441 ggtacgactg ctgctctcaa tatcctccac ttcattgaag aacagtttcc ccgtacggta
    13501 cacggctgac tttttaaagt cctctttcag tctggcatgc tcaatagtgc cgctgccatc
    13561 atgctgtgca tcaatgtctg cctgctcaag ggaagcgtgc aacattttta tatacgccgg
    13621 ttcattgatg gtgtgatagt gcagttgttc aagtatgctt aaatcccttg tactgttatc
    13681 gaagcgacga gtgaagctct tctccctgtc ataaacaaac ggataatagc gagcaccacg
    13741 accaataagt tgcgcctcac tgtccgttcc tgtcttacta ctgctcgcct gttcgctgat
    13801 gcgaacaata tcgtagaggt tgagaacatc ccagccttca ttgattttcg ccacagcaaa
    13861 gacagcccgg acagggttgt cgacgtcttc cagcgtattc agcaacaccg gattttcttc
    13921 cagcaggtca gacctgttga cattcagaag attaaagtcg ttaaagtcct cctggatctg
    13981 tccgataacc gtgattaaat cactgtcatc gtaccgctgt atcactttat gccagatact
    14041 ggtgtcagag cttagccgga cttttttatt agacagatgg cgtctgacgt tctcaggcgt
    14101 cagcccggca atcagttgcg aaaactccgc ttgtttcgct ttcgatacag caattttatt
    14161 cgacttgaaa agcaggaccg gcttgaagtc agtgataccg tgatcctccg ccgtcagctt
    14221 gcgatactgg cttaacaaca cggcatcaag cattttgtcg tcgtcattct ggttggcctc
    14281 cagcaacata acttttttcg agtaaccgtc tgacataaac ttcttcaggt catactgata
    14341 cacaactttg tcacggtatt tctgccagat atctttgttc gcaagattaa tagtcgcggt
    14401 gaattcgaac agacggttat ccggtctcag gtcgagtatg ttctcaatcg tacgctccca
    14461 tgtatgttcc tcgttttcag atgtgttttt tgccttgcct ctggctttag taccggcatt
    14521 gaagtgatgc gcctcatcac caagcaacac aagaggaagc tctttcaaat cctcactggt
    14581 gataccattc tcacggtagc tactcagttc atcgtgcatc ttatgaatgg tcgagagcct
    14641 gaggtaaatc gtgttgcgtt caggcacctg cgggaatata tctacaggga tgatagtgat
    14701 tttttccccg tcaatttcca ggttctgact gaacaggtac tttggcgatt gtgggttaag
    14761 cagattctcc cgggttttct gaataatcgc gtcggtatgc acgaaaaaga tgaagttctg
    14821 gtaactgtac tctttaaaca ggtaaagtat cgctccagcc attaccattg tttttcccgc
    14881 tccagtggcc atatggaaca acagatgacg gtaatgctgt aatgcctctt ccggattacg
    14941 ctgcgtgtaa tgaagataac gcaacgccgc gtgttgatac ggtcgtaaat catccttcag
    15001 gttcgcctga acataatccg gtgtctgata gttgtcatgg aacaaccctg gttcatcaaa
    15061 ctgcttcagc cggttataca acagcttttc gctgccagcg gactctgctt gcttagccat
    15121 atagttactc cataccatag aaactgcggg taaaagcttt gtcgctctct gaaatattca
    15181 gatcgctgtc atccatatca gaagcgttca catagagctg atttttatcg agaatttcaa
    15241 tcaggagctt cttctgctca tgcagttcca gttcacagaa tgcgacttta cggaagatgc
    15301 cgtccacttc atattcagcg gaaagcacgt tctcaagtgc gacctggtag ttgagatgcg
    15361 cctctgtttt catcactgca aagagctttt cgagttcttc actcgactgc gctttccgaa
    15421 tttcattcac gaagagggca ttaagctcca tcagttcgac atagagaaaa ctgccgccgc
    15481 actgccagcc aacatctttt gaaatgccgc cttgttctcc atcaataacc ttttgcatac
    15541 gtggaacagt aatgttatgg atgtaatcca tctgctcaat accgatatag cgacgcccca
    15601 ttttgtgggc aacagcggcg gttgttccac tgccaagatg gaagtccata accagatctc
    15661 cgtcattcga tccaatatga aggattcttt gtattaatct ctctggcttt ggtgtcgaaa
    15721 aaacactatc catattaagg gcattaattt cttttttggc ttcttgatta tgccctactt
    15781 cagaatttgg ccaccacgtc cagggcattc gtcctttaac atcttttaag tatgttttac
    15841 gtctgggcat agcgtcacca tttacaccaa accacatttt tccttcttct ttttgtctta
    15901 tcatctcaga ctcaatattt ttccagcacg ttccttctgg tgggtagtat gatttacctg
    15961 aaggtgtttt tatttcatac atctgattag gtctgaaacc ttgcgcagta aagtctgatg
    16021 acacccatgg tccattaggg tcattgtctg ggttgctata tctatttttc tgctcttgag
    16081 ttaaaggtat taaatttatt ttagctctaa acaattctgg atttaaagaa tagacaagaa
    16141 tatgatcatg cccggcccct aatggaaccc ttgcatctgg tgatgttctt ttttgccaaa
    16201 taacatcagc cacaaaaccc aacccagtat cttgatccat aagcacttta agatagtgtg
    16261 cttcattatc atctaaatta atccagatac tgccatcttc tgaaagaagc tcttttgcta
    16321 tttccactct atttttcata aaggacaacc acgttgagtg gttaaaactg tcgttatatt
    16381 taaagctatc agttccagta ttatacggtg gatcaatata aatcagcttt acttttcctg
    16441 catagcgttg tttcagagag tgaagtgcta tcaggttgtt tccttttatt attaaattat
    16501 cactttcgaa agaggtgata ttttcttcaa caccatattt tgtatatcgt tttatattag
    16561 taaatacttt attggaaaac agccgatcga tttcatcacg agcgattatt tcattgtaat
    16621 agatttcatc tttaccctgt tcttcttttg tcatgccgcc ttcaaggaca caatccttaa
    16681 agggaaagtc cagcacaacg tctgaattat aatcaaggta tttaccttcg cttgttaaac
    16741 caatcttatt acgatatctg gtgtaactgt ttgcccagta ttctttatat ttcaggaggc
    16801 tgcacagcct ttcaaattca aaaattaaaa caccatcaat ttcagtacca tatactgatt
    16861 tgattttttc atttgcaacc agagccttga taatttccgg ttgcttgcgg ttaatggcat
    16921 ctatcacaac agagcgctgt aattcctgcc cgttccagaa caaaggaaat ccacgtaaaa
    16981 cagaattcag ttcccgaatt aattca
//
LOCUS       DAZGSP010000110        16885 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZGSP010000110 DAZGSP010000000
VERSION     DAZGSP010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.111"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            27..248
                     /locus_tag="ACHK74_004040"
     CDS             27..248
                     /locus_tag="ACHK74_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222773.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            178..696
                     /locus_tag="ACHK74_004041"
     CDS             178..696
                     /locus_tag="ACHK74_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFP0222774.1"
                     /translation="MAPIACSWRRIPKFWNWIPASKIEKETRMYGTCETLCRELAAKY
                     PGDAPLMLVIWSPEEIQALADGMDIALSDHEIRTVLARLEDIPEDQRTESGISSGVAM
                     EIINNVRENRQVTVPAELLASLIQTAEQALWKREWAARDNGLVVPECVTRRQAVVNQA
                     RALLKNNTHEND"
     gene            741..1511
                     /locus_tag="ACHK74_004042"
     CDS             741..1511
                     /locus_tag="ACHK74_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222775.1"
                     /translation="MNKTLNALVYRHARNLLLAQGWPEETDIEQLNPHYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEEEIRAVLDAVRDAVCSVSCRVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDYPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSECVEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            1927..2352
                     /locus_tag="ACHK74_004043"
     CDS             1927..2352
                     /locus_tag="ACHK74_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0222776.1"
                     /translation="MQYAKSVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            2399..2818
                     /locus_tag="ACHK74_004044"
     CDS             2399..2818
                     /locus_tag="ACHK74_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFP0222777.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNCLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRVMRQALDGENG"
     gene            2818..3009
                     /locus_tag="ACHK74_004045"
     CDS             2818..3009
                     /locus_tag="ACHK74_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222778.1"
                     /translation="MNISKETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRHR"
     gene            3207..3423
                     /locus_tag="ACHK74_004046"
                     /pseudo
     CDS             3207..3423
                     /locus_tag="ACHK74_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426396.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4091..4321
                     /locus_tag="ACHK74_004047"
     CDS             4091..4321
                     /locus_tag="ACHK74_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222779.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            4373..5734
                     /locus_tag="ACHK74_004048"
     CDS             4373..5734
                     /locus_tag="ACHK74_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFP0222780.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPCREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQRQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            5781..6344
                     /locus_tag="ACHK74_004049"
     CDS             5781..6344
                     /locus_tag="ACHK74_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFP0222781.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELLRGTFAYTDVPTMIIRLRA"
     gene            6344..6592
                     /locus_tag="ACHK74_004050"
     CDS             6344..6592
                     /locus_tag="ACHK74_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222782.1"
                     /translation="MTQGWREDKCPSVRAGKKPRAGGAERGRAHAPPLSLRGRSDGGF
                     CLFRPVICLLLMMLLFLLLLLLLLLLLLLLLLFFFLPL"
     gene            6496..6762
                     /locus_tag="ACHK74_004051"
     CDS             6496..6762
                     /locus_tag="ACHK74_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222783.1"
                     /translation="MSVTDDVVVPATATATATATATATALLFPAALILTTAVLSCLPG
                     APSAPGCLAASGRQARLRQAAAGAPASLRRTRWRGFGLSLRSDA"
     gene            7233..7754
                     /locus_tag="ACHK74_004052"
     CDS             7233..7754
                     /locus_tag="ACHK74_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0222784.1"
                     /translation="MAVRGINKVILVGRLGKDPDVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPEPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            7812..8045
                     /locus_tag="ACHK74_004053"
     CDS             7812..8045
                     /locus_tag="ACHK74_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFP0222785.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDCAGYWMNRYIRDFGILK"
     gene            8106..10064
                     /locus_tag="ACHK74_004054"
     CDS             8106..10064
                     /locus_tag="ACHK74_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP0222786.1"
                     /translation="MSVTKSEPKSTRKASRKLAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVCVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMESVGFCREDAGTYRCLPEPESVLTEAEE
                     ERLNELMARYDALENQCEESDLLAAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDPAADADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECEHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPVGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMGSPRPQTDATERADNLADAA"
     gene            10119..10553
                     /gene="psiB"
                     /locus_tag="ACHK74_004055"
     CDS             10119..10553
                     /gene="psiB"
                     /locus_tag="ACHK74_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFP0222787.1"
                     /translation="MKTELTLDVLQTMSAQEYEDIRAAGSGERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQIRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFVPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            10550..11269
                     /locus_tag="ACHK74_004056"
     CDS             10550..11269
                     /locus_tag="ACHK74_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFP0222788.1"
                     /translation="MSVRSQALVPLSAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRWISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERRRHKSALVMQKYSRQQAREAEQKTRAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            11266..11580
                     /locus_tag="ACHK74_004057"
     CDS             11266..11580
                     /locus_tag="ACHK74_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="theronine dehydrogenase"
                     /protein_id="HFP0222789.1"
                     /translation="MKWRYSLRWKLPYPCPGEHELVSEVVEAGQPAPASVMSRWVAGA
                     GYAVCLDFISDRPVRRWSEERKAAVRRRNLEKRINRHAPLFADELIARELAERPDYFQ
                     GK"
     gene            11730..11885
                     /locus_tag="ACHK74_004058"
                     /pseudo
     CDS             11730..11885
                     /locus_tag="ACHK74_004058"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            11830..11952
                     /locus_tag="ACHK74_004059"
     CDS             11830..11952
                     /locus_tag="ACHK74_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0222790.1"
                     /translation="MVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK"
     gene            complement(12406..12579)
                     /locus_tag="ACHK74_004060"
                     /pseudo
     CDS             complement(12406..12579)
                     /locus_tag="ACHK74_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12879..13181
                     /locus_tag="ACHK74_004061"
     CDS             12879..13181
                     /locus_tag="ACHK74_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222791.1"
                     /translation="MSAFSFNTAYQPTGDQQKDAIAQIDIMQNRAVQANLAYQSSLDA
                     ETLLKQLWQINDELGELLDSVENHTPVIRKKASDLSALMMLFSQQAGQPMTMLLIC"
     gene            13337..13624
                     /locus_tag="ACHK74_004062"
     CDS             13337..13624
                     /locus_tag="ACHK74_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222792.1"
                     /translation="MSTRNIHVNTATYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRTGLDIRAIC"
     gene            13743..14564
                     /locus_tag="ACHK74_004063"
     CDS             13743..14564
                     /locus_tag="ACHK74_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP0222793.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLREEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVPGVFD
                     KVTENMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRLEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRGITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(14861..15451)
                     /locus_tag="ACHK74_004064"
     CDS             complement(14861..15451)
                     /locus_tag="ACHK74_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFP0222794.1"
                     /translation="MVRTTNLLSLFVEWVKLFPDDVTRGEKMRKWLLTICLMFINVTC
                     KAGDCFDLAGRDYKIAPDLLRAISWKESRNRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTADPCLNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLA
                     YASDVYRIYSGIKNSKGIQLRASEKPLSKMNSSQKN"
     gene            15786..16169
                     /gene="traM"
                     /locus_tag="ACHK74_004065"
     CDS             15786..16169
                     /gene="traM"
                     /locus_tag="ACHK74_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFP0222795.1"
                     /translation="MARVILYISNDVYDKVNAIVEQRRQEGARDKDISVSGTASMLLE
                     LGLRVYEAQMERKKSAFNQIEFNKLLLECVVKTQSSVAKILGIESLSPHISGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDDE"
     gene            16360..16812
                     /locus_tag="ACHK74_004066"
     CDS             16360..16812
                     /locus_tag="ACHK74_004066"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222796.1"
                     /translation="MCAMDRRERALIRQLHFPNDLRANISLLKYPVCIRFEDGSFIGT
                     NHSFERFIKLSYCSCDEWFEHLTLESKLQLSRSEMDSYFSTYGVSYINNVFISAMSWS
                     MVIETLNTNEGCFFVWRFIESNDKNISTSLISKSNDGLMFFFWGICRY"
BASE COUNT         3926 a         4291 c         4865 g         3803 t
ORIGIN      
        1 actggtttga accggaggct gcgtaaatga actatgccgg acacgaaaaa cttcgcgccg
       61 acgtggcgga ggtggccaac accatgtgtg accttcgggc caggctgaac gatatggagc
      121 atcgctgtcg ctttgattcc gatgtgcttg ttgaacgcct gacacgccag acgctgtatc
      181 gcgccaatcg cctgttcatg gaggcgtata ccgaaattct ggaactggat tcctgcttca
      241 aagattgaga aggagacgag aatgtacgga acatgcgaaa cgctatgccg ggagctggca
      301 gcaaagtatc cgggagacgc gccactgatg ctggttatct ggtccccgga agaaattcag
      361 gccctcgctg acggaatgga tattgccctg tccgatcatg aaatcagaac cgtcctggcg
      421 cgcctggagg acatcccgga agaccagcgg actgaatccg gtatttcttc cggcgtggcg
      481 atggagatca tcaataacgt gagagaaaac cgccaggtga ccgtccctgc tgaactgctg
      541 gcgtccctga ttcagaccgc tgaacaggca ttgtggaagc gtgaatgggc cgcccgtgat
      601 aacggcctcg tcgtcccgga atgcgtcacc cgccgtcagg cggtggttaa tcaggcccgc
      661 gccctgctga aaaacaacac acacgaaaac gactgatgtt atcgccgctt tcgggcggcg
      721 attcagggag accattaatc atgaacaaaa cgctgaatgc actggtttat cgccacgccc
      781 gtaacctgct gctggcgcag ggctggccgg aagagacgga tattgaacaa ctgaatccgc
      841 actatccggg ctggatcagc atttacgtgc ggctggatgc gccccggctg gcgacgttac
      901 ttatcaaccg tcacggcggc gtactgccgc cgctcctggc ctctgccatt cagaaactga
      961 ccggaaccgg agcggaactt gttctgtccg gcagtcagtg gcagtcgctg ccggtacttc
     1021 cggcagacgg aacgcaggtg tctttcccgt atgctggtga atggctgacg gaggaagaaa
     1081 tcagggcggt tcttgatgcg gtgcgcgatg cagtatgcag tgtcagttgc cgggtggcag
     1141 aagatgcgcg gcgtatccgt gcggcgctga ccaccaccgg tcagacgttg ctgacccgcc
     1201 agacgcgccg ctttcgtctg gttgtgaagg aaagcgatta cccctgctgg cttgatgaag
     1261 atgacgaaaa tctgcccgtg gtgctcgatg ccatcctgaa ccggggagca cgtttttcgg
     1321 cggtggaaat gtacctggtc agtgagtgtg tggagcatat cctgtccagt gggctggcct
     1381 gcgatgtgct gcgtataccg gatgaaccgc cccgccggtg gtttgaccgt ggtgttctgc
     1441 gggaggtggt ccgggaagcc cgggccgaaa ttcgcagcat ggcggatgcc ctggcaaaaa
     1501 tccggaaatg atgacgctgg cggggaaaaa tctccgcctt ttcctgccgg tccccggaca
     1561 gagcacaaag gatgtcgccg caggctccgc tttacccggc catgcggggc atggccttgt
     1621 gggttttcag ttatgtggcc tcagcgtcgt gtgcgggctg tgccgtgcct ccatcgtagc
     1681 cgggcttctg cgggcgggtt gcaaggtaag gtgaaccgct gcggtcaccc ggtccctcct
     1741 tcccgtctgc cgtgattttc cggtcgttca cccgctcaga tttcgcggtc tcaccctcca
     1801 actcccgtca gccgtgtgcg tcaggctgcg gcttcccttg cgcccttgca accctgcagg
     1861 cccggcaaag atggaggcac ggcaccgccc ggtgtcgcgg aatatgtgaa caacggagga
     1921 aatcccatgc agtatgcgaa atctgtcact ctgaacgttg aagagtgcga ccgtctctcc
     1981 tttctgcctt acctgtttgg caacgatttt ctgtatgccg aggcgtatgt gtacgcgctg
     2041 gcgcaaaaaa tgatgccgga atatcaggga ggcttctggc atttcatccg cctgccggac
     2101 ggtggcggtt acatgatgcc ggatggcgac cgttttcatc tggtcaacgg tgaaaactgg
     2161 tttgaccgca ctgtgagtgc tgatgccgca ggtatcatcc tgacttccct tgtgattaac
     2221 cgccagttgt ggctgtacca cgacagcggg gatgcgggac tgacacacct ttaccggatg
     2281 cgcgatgcgc agttgtggag ccacatcgaa tttcaccccg aatgtaacgc gatttacgca
     2341 gcactggact gattaacgga cggggcggcg atgccgcccc tgtaaaggag acgagaatat
     2401 gtactgtact gttaaagaaa tcatccgcga tgtactggat acagacgtgc cggacagtga
     2461 atgcgttttt gccgtggtgc tgacccgtgg ggatgtgcgc cacatagccc aggactggag
     2521 tctgacagac gatgagctgg aaaccgtcat gcagcggctg gacgatgctt ttgtatatgg
     2581 cgcatgtgac agagttgttt ccgatattgt caatgaactg atggaggaaa agcgcgtaaa
     2641 ctgtcttgta acagtccctg cggtgctgct ggaaaaagtg atggtgatgg ctggtagtga
     2701 aatttatcgc ctccatgctg tagggagcga gaacgggggc gacggtgacg catttgtcag
     2761 ggaagaacgt gagattatgc gggtgatgcg tcaggcgctg gacggggaga atgggtaatg
     2821 aatatcagca aggaaacccg cgaaattctg cgtaattaca gggccgtgat taatgcgcgg
     2881 cgtcgtgaaa tggggcagaa accgctcacc accgcgcaga ttgttgatga aatctgcgat
     2941 tttgtggcga atcagcaggc ggttttcctc ggtggtcact acatccttca gggcagcagg
     3001 cacaggtgat acagggcagg cggtggaggc cgcctgtcag cccgcgcgaa aacccctggc
     3061 aggctggcgc ctgccggaag ggatgcgggc cacgctggct cgtcgcccgt tacccttccc
     3121 gtttgtgatg tgtctgactc aggagtgttt ctttcttatg ttatggaact gttcctgatg
     3181 ttgttgttgt tcttttctct gctgctctga ttctgactac tgcagtgttg tcctgtctgc
     3241 cgggcgcacc ttcggctccc ggctgcctgg cggcatccgg tcgtcaggcc cgactccgac
     3301 aggcagctgc tggcggcatc cggtcgtcag gcccgactcc gacaggcagc tgctggcgca
     3361 cccgccagtc tccgccgaac ccgctggcgc ggtttcgggc tgtcgctacg gtccgatgcg
     3421 tgaaaacccc tggcttgcag cgaccggaac ggtcgcgccg cccttgtcgc gccgtggcct
     3481 gcgctcccgg catcccggca aaaagacgcc gggatgtaag gagcaacgcc gtttcgcaaa
     3541 ccgcgcttca cgtcgttgcg gcgggtccct gctcattgcc tgccgggtgc cacacacccg
     3601 tatgagtccc tgtcgccggg ggctgataca gccacaacct gccgcactga ctgtccaggg
     3661 taaatgaaca gcccacaaaa ttttttacct aaaacccggt aaaaaatttt gcgttctgcc
     3721 caggacaggt gcgtcaggcc gtggcagtga tgccccttgc ggcgaaggtt ctcaaacggg
     3781 tgaagttcat accacaggga atacagcagc aggttcccgc gaaacgtcaa aaccgcggct
     3841 cccgaaactc aacgggtgat gttctttaaa aatcaggatt tatttcaggc gccggcaata
     3901 cgcatttatg tgttctgtca gcatcctgct ggcaggtaat gagaacgcag gaacagaagg
     3961 aggaatacac cggagaacgt cagacgcaga tatgaaaaaa gcaggtcagg gacctgctta
     4021 atccacgccg aagagactgt gcaaccagtc tttaaggcac tgttaccaac ctgtcataag
     4081 gtatccattt atgactgtaa gcattgtttc accttccgca gcggctgtaa agccccgccg
     4141 tcatccccgt ttcagacgcg aagacatccc cgcgccggaa attgaccccg tgctgaaagc
     4201 cttcgggcga cacatagccc ggagttttca ccgggggcgt ggtgtacaca tccccgccat
     4261 gaaaaacacg gctttcgggc aggtgctccg cacactcgaa ctgaaacgcg cttttaactg
     4321 atgaggtgag ccgccccgcg aggggcggca acagacggaa ggagaacatt ttatgacatt
     4381 agcagcaatc accatgaccg ccccggaagc cgccagcccg gtgcagatgt accgcgcgac
     4441 ctactcaccg gatgacaaca aactgcgcct gtatgccgcg tcacgtcttg acccggagac
     4501 gtataaaaaa gtgcatgatg ccggttttcg ctgggcaccc aaacaggcgc tgtttgtcgc
     4561 gccagcctgg acaccgtgcc gggaagacgt gctcctctca cttgccggag agattgagga
     4621 tgaagacagc acgctcgctg aacgtcagga agcacgggcg gagcggttta ccggatacag
     4681 cggaaagcgg gccagtgaat ccgcacaggc acttgatgaa gtggaaagac tggccgcgat
     4741 gatcccgccc ggtcagccca ttcttgtggg gcatcacagc gaacgccgcg cccgtcgtga
     4801 tgcgcagcgt attgaaaacg gtatgaaacg tgctgtgatg ctctttgaac gtgcggaata
     4861 ctgggaagag cgggcgcggt cggcactgct tcacgcgaag tataaagaac gtccggacgt
     4921 tcgctggcgt cgtatcaaaa aaatcgaagc tgatttgcgc aaggcggaaa agaccatcgc
     4981 acagtcgcag aaatatctga cgatgtggcg ggctgaatcg ctggatctga atatggcaaa
     5041 actcatcagc agtcatgacc atatcagcgc ctgtttcccg ctggatacgt atccgcgccc
     5101 ggcagaaaaa agccagtatg aaggaagtcg atcgttatgg tcggccctgg atgatgacat
     5161 catcaccacg gagcaggccc gcgaaattgc gatccgctgt catgaacggc agatccagcg
     5221 tcagcaacgc tgggttaacc actatcagaa ccgcctgatc tatgagcgtg ccatgctgga
     5281 cgaaagcggc ggcgtggtta cccggacaca ggattttgag ccgggtggac aggttttcag
     5341 ccggggcgag tggctgacca tcatccgcgt gaacaaaagc aacggggcgg tgagttcagt
     5401 cacaacgccg aattacagtt ttctcgggta cagcggcacg atgaaagtga cgcccgatcg
     5461 cattacggac tacaaagcac catcggcaga agaggctgcc gtcgccagtc aggccgcgaa
     5521 gcgtccgccg gtagtcaact atccggggga aggtttccgg gaaatgacaa aggcacagtg
     5581 ggccgccctg ccccgggact gtaaggccgt gcgcagtgtg gcagaagcag aagaccacgg
     5641 ggcataccgc taccgccgca caatggacaa taatttccgc ctggtgaatg tgtatatcac
     5701 cgacatgaaa atcacggaaa tcccgcagaa ataaggtata tccccccggg aaatccccgg
     5761 gggatgacgg agataatgac atgcacagtc agttaagaga acgtatccgg ctgatgcgcg
     5821 caaggctgga taacgccgcg ccggttgctg aaatccgggc tgaatctcag ctttttgtga
     5881 ctcctgcccc ggtctgtgat cgcctggtga cgctggcgga aatcagcaac cgtgatcaca
     5941 ttctggaacc ctctgccggc accggggcca tactgcgggc gattcgggat accgcgccgg
     6001 aggccatgtg tgatgcggtg gagattaaca gcgggctggt ccggtatctg cgggaaaatt
     6061 ttaacggtgt cagggttcag tgtggcgact tcatggaatg gcagccggtg cagtattaca
     6121 gccggatcat tatgaatccg ccgttcagtc acgggcagga tatccggcat atcctgcggg
     6181 ctttttccct gttgcgtccg gggggcgttc tggttgctgt ctgtctcaac gggccacgcc
     6241 agcaggagaa gctgttaccg ttttctgacg tccgcgagga gctgctgcgc ggcacgtttg
     6301 cttataccga tgtcccgacg atgattattc gtttgcgtgc ctgatgacac agggctggcg
     6361 ggaggataaa tgcccgtctg tccgcgccgg gaaaaaaccg cgcgccggtg gcgctgaaag
     6421 gggccgcgct cacgcgccgc ccctttcatt acgcggacgt tctgatgggg gcttctgtct
     6481 tttcagacct gtcatttgtc tgttactgat gatgttgttg ttcctgctac tgctactgct
     6541 actgctactg ctactgctac tgctactgct cttctttttc ctgccgctct gattctgact
     6601 actgcagtgt tgtcctgtct gccgggcgcc ccttcggctc ccggctgcct ggcggcatcc
     6661 ggtcgtcagg cccgactccg acaggcagct gctggcgcac ctgccagtct ccgccgaacc
     6721 cgctggcgcg gtttcgggct gtcgctacgg tccgatgcgt gaaaaccccg ggtttgcagc
     6781 gaccgttccg gtcgcgccgc ctctgtcgcg ccgtggcctg cgctcctggc atcccggcaa
     6841 aaggccgccg ggatgtaagg cgcaacgccg tttcgcaaac cgcgcttcac gtcgttgcgg
     6901 cgggtccctg cccgttgttg tgtccggtgc cattcacctg tttaagcccc tgtcgccggg
     6961 ggctgataca gccacaacct gccgcactga ctgtccaggg taaatgaaca gcccacaaaa
     7021 ttttttaccc aaaacccggt aaaaaatttt gcgttctgcc caggacaggt gcgtcaggcc
     7081 gtggcagtga tgccccttgc ggcgaaggtt ctcaaacagg tgatggtgaa taccggaggg
     7141 aacaaagagc aggtccccgc aaaacgtcaa aaccgcggct cccgaaactc aacagatgat
     7201 gggggctgaa aacatcaaca gaaggagaca tcatggcagt tcgtggcatt aacaaggtca
     7261 ttctcgtggg acgtctggga aaagatccgg atgtccgtta catccccaac gggggcgcag
     7321 tggcaaatct gcaggtggcc acgtcagaaa gctggcgtga caaacagacg ggggagatgc
     7381 gggagcagac agaatggcac cgcgtggtgc tgttcggcaa gctcgcggaa gtggcaggtg
     7441 aatatctgcg taagggcgcg caggtctaca tcgaggggca actccgtacc cgtagctggg
     7501 aagataacgg tattacccgt tacgtcactg aaatccttgt gaaaaccacg ggcaccatgc
     7561 agatgctggg gagtgcacca cagcagaacg ctcaggcgca accggagcct cagcagaatg
     7621 ggcagccaca gagtgctgac gcgacgaaaa aaggtggcgc gaaaacgaaa ggccgtggac
     7681 gtaaggccgc gcagccggag cctcagcagc cgtctgaacc cgcgtatgac ttcgatgacg
     7741 atattccgtt ctgaacgggc tgactgtgat aaccgccccg tcctgcgcgg ggcatcaccg
     7801 gagaaatgag gatgagcgaa tatttcagaa tacttcaggg actgccggac ggctccttta
     7861 cccgcgaaca ggcggaagcc gttgccgcac agtaccggaa cgtctttatc gaggatgatc
     7921 agggaacgca ttttcgcctg gttgtccgtc aggatggcac gttgatctgg cgctcctgga
     7981 attttgagga ctgtgccggg tactggatga accggtacat cagggatttc gggatcctta
     8041 agtaagagag gtgccggacg gggatcacgt ccggcagaca ttaactacac aataagggga
     8101 tagtcatgtc agtaacaaag tctgaaccaa aatctacccg taaagcaagc cgtaaacttg
     8161 caaaaacgca ggaaactgtc ctgtctgccc tgctggcgca gacggaggaa gtgagcgtgc
     8221 cgctggcctc actgattaaa tcaccgctga atgtgcgcac ggtgccgtat tctgcggagt
     8281 ccgtcagtga actggctgat tccattaagg gagtcggcct gctgcagaat ctggtcgtgc
     8341 ataccctgcc tggtgaccgt tacggtgtcg ccgcaggtgg tcgccgactg gctgcactca
     8401 acatgctggc agagcgtggc atcattccgg ctgactggcc tgtctgtgtg aaggtcattc
     8461 cgcaggaact ggcgacggcg gcatcaatga ctgaaaacgg tcatcgtcgg gatatgcacc
     8521 ctgctgaaca gattgccgga ttccgcgcaa tggcgcagga aggcaaaaca cctgcacaaa
     8581 tcggtgattt gctgggctat tcaccccgcc acgttcagcg aatgctgaaa ctggcagacc
     8641 ttgcaccagt catactggat gcgctggcag aagaccgcat caccaccgaa cactgtcagg
     8701 cgctggcact ggagaacgac accgcgcgtc aggtgcaggt gtttgaagcc gcctgtcagt
     8761 cgggatgggg cggtaaaccg gaagtacaga ccattcgtcg tctggtgacc gaaagtgaag
     8821 tggcggtggc aggcaacagt aaattccgct tcgtgggggc tgatgctttc tcacctgacg
     8881 aactgcgcac cgatttgttc agcgacgacg agggtggcta tgtggactgt gttgcgctcg
     8941 atgctgccct gctggaaaaa ctccaggctg tcgctgaaca ccttcgggaa gctgaaggct
     9001 gggaatggtg cgccggacgt atggagtctg tcggtttctg ccgtgaggat gccggaacat
     9061 accgttgtct gccggagccg gaatcggtgc tgaccgaggc ggaagaagaa cgcctgaacg
     9121 aactgatggc acgttacgac gcactggaaa accagtgtga ggaatctgac ctgctggcag
     9181 cagaaatgaa gctgattgac tgcatggcga aggtcagggc gtggacgccg gagatgcgtg
     9241 ccggaagtgg cgtggtggtg tcctggcgtt atggcaacgt atgtgtccag cgtggtgtgc
     9301 agttgcgcag cgaggatgac ccggctgccg acgctgaccg cacggaacag gtgcaggaga
     9361 aagcgtcagt ggaggaaatc agtctgccac tgctgacgaa aatgtcttca gagcgcacgc
     9421 tggcagtcca ggcggcgctg atgcaacagc cggacaaatc cctggcactg ctggcatgga
     9481 cgctctgcct gaatgtgttt ggcagcgggg cgtacagtaa accagcacaa atcagcctgg
     9541 aatgtgaaca ttattcgctg accagcgatg cgccatcggg gaaggaaggt gccgcattca
     9601 tggcgctgat ggcagaaaaa gcccgtcttg ctgccctgtt acctgtggga tggtcacggg
     9661 acatgacgac attcctgtca ctcagtcagg aggtgctgtt atccctgctc agtttctgca
     9721 ccgcatgcag ccttaacggt gtccagaccc gtgagtgtgg tcacacgtca cgcagtccgc
     9781 ttgactcgct ggagagcgcc atcggctttc acatgcgtga ctggtggcag ccgacgaaag
     9841 caaacttctt cggacacctg aaaaagccgc agattatcgc agccctgaat gatgccggac
     9901 tgtccggtgc cgcacgggac gcggagaaga tgaagaaagg cgatgccgct gaacatgcag
     9961 agttccacat gaaagacaac cgctgggtgc ctggctggat gggttcacca cgtccacaga
    10021 cggatgccac tgaacgcgcc gataacctgg ctgatgccgc ctgatgaaca accacaccgc
    10081 cccgccggag acggggcggc agcaagggag ataccgtgat gaaaactgaa ctgaccctgg
    10141 atgtattaca gaccatgagc gcacaggaat atgaagatat ccgggctgcc ggaagtggtg
    10201 aacgtcgtga gctgacacac gccgtgatgc gggagctgga tgcgccggat aactggacga
    10261 tgaacggcga gtacggcagc gagtttggcg gctttttccc cgtccagatc cgtttcacgc
    10321 ctgcccacga acgttttcac ctggcgttat gttcaccggg tgacgtctcc caggtctggg
    10381 tgctggttct ggtgaatgcc ggcggtgagc cgtttgcggt ggttcaggtg cagcggcggt
    10441 ttgtaccgga agccgtcagc cattcactgg cactggcggc gtcactggat gcgcaggggt
    10501 acagcgtcag tgacatcatc catatcctga tggcggaagg aggtcaggca tgagcgtacg
    10561 ttcacaggca ctggttcccc tcagcgcaga gcaacaggcc gcatggcggg cggtggcgga
    10621 gacggaaaaa cgccgccatc agggaaacac gctggcggaa tatccgtatg cgggggcgtt
    10681 tttccgctgt ctgaacggaa gccgctggat ttcgctgtct gacctgcgat ttttcatgcc
    10741 gtccctgacc gcagaagaac tgcacggcaa ccgtcttcag tggctgtatg ccatcgatgt
    10801 gctgattgaa acacagggcg aggtctgcct cctccccctt cccggcgatg cagcagaacg
    10861 cttgttcccg tcggtgcggt tccgtgtcag ggaacgccgt cgccataaat ctgcgctggt
    10921 catgcagaag tacagccgcc agcaggcgag ggaggcagag cagaaaaccc gtgcgtacca
    10981 ggcgctggtt gctcaggcag aaatcgaact ggcgttccat tcaccggaaa cagtcggaag
    11041 ctggcatgca cgctggagtg accgggtggc agagcatgac ctggaaaccc tgttctggca
    11101 gtggggcgag cgttttccgt cactggccgg aatggaacgc tggcagtggc aggatatgcc
    11161 gttctggcag gtcatcgcag aggcaggtat ggcggcaagg gaggccggtc atgccgtccg
    11221 tgagatggag cgatggatgg tgccgaacaa actccgggag gcggcgtgaa gtggcgttac
    11281 tcactgcgct ggaaactgcc ttatccctgt cctggcgagc atgaactggt ttcagaagtg
    11341 gtggaggcgg ggcagcctgc accggcatcc gttatgtcac gctgggttgc cggagcaggg
    11401 tatgcggtct gtctggattt tatttctgac cgcccggtgc gccgctggag cgaagaacgt
    11461 aaggctgcgg tacggcggcg gaatctggag aaacgtatca acaggcatgc accactgttt
    11521 gcggatgaac tgattgcccg tgaactggct gaacgaccgg attattttca gggaaagtga
    11581 gtgtggtcag tacgcaggta tatgggctat gatgtgcccg gcgcttgagg ctttctgcct
    11641 catgacgtga aggtggtttg ttatcctgtc gtgtggcaga aggacaaaag ccccgtagtt
    11701 aatttttcat taacccacga ggcctctgca tgtctagtcc acgtcagtat ggcctcttac
    11761 cgtgcaaatt gcaaggagga ggagtaaacc atgaaactac cgggaaacgc ccttatctgg
    11821 tgcgtattaa tcgtgtgctg cacgttgttg atattcacac tcctgacccg gaatcgcctg
    11881 tgcgaagtcc ggctgaagga cggatacagg gaagttacgg caacaatggc ttacgaatcc
    11941 ggcggtaagt agcaacctgg aggcgggcgc aggcccgcct tttcagggct gatgctggtc
    12001 tgactgcact gaagcgcctt tataaagggg ctgctggttc gccggtggcc cctttctcct
    12061 tgctggcatt gtagcagcat gaacaaccag actgtcagga aggccacttc cctccgggaa
    12121 cgggaatgaa cgtgcgcctg cggcgctcgt tcttttcccc cgccttctct gtttatggcc
    12181 agttgtcaga aatgacatta cctttcccgt gattattctc tttttcttcc tgctctgatt
    12241 ctgactactg cagtgttgtc ctgtctgccg ggcgcacctt cggctcccgg ctgcctggcg
    12301 gcatccggtc gtcaggcccg actccgacag gcagctgctg gcgcacctgc cagtctccgc
    12361 cgaacccgct ggcgcggttt cgggctgccg ctacggtccg atgcgtgaaa accccgggct
    12421 tgcagcgacc gttccggtcg cgccgcctct gtcgcgccgt ggcctgcgct cccggcatcc
    12481 cggcaaaaag acgccgggat gtaaggcgca acgccgtttc gcaaaccgcg cttcacgtcg
    12541 ttgcggcggg tccctgcccg ttgttgtgtc cggtgccatt cacctgttta agcccctgtc
    12601 gccgggggct gatacagcca caacctgccg cactgactgt ccagggtaaa tgaacagccc
    12661 acaaaatttt ttacccaaaa cccggtaaaa aattttgcgt tctgcccagg acaggtgcgt
    12721 caggccgtgg cagtgatgcc ccatgcggcg aaggttctca aacaggtgat ggtgaatacc
    12781 ggagggaaca aaaggcaggt ccccgcaaaa cgtcaaaacc gcggctcccg aaactcaaca
    12841 gatgatgggg gctgaaaaca tcagatacag gaggaaacat gagcgcattt tcttttaaca
    12901 ccgcttatca gccaacaggc gatcagcaga aagacgcaat cgcacaaatc gacatcatgc
    12961 agaatcgtgc tgttcaggca aatcttgcat accagagcag cctggatgcg gaaacgctat
    13021 tgaaacagtt atggcaaatc aatgatgaac tgggggaact gttggatagc gtggaaaatc
    13081 acacgccagt tatcagaaaa aaagccagtg atttgagcgc actgatgatg cttttcagcc
    13141 aacaggccgg tcagcctatg actatgttgt tgatttgtta atgataatca ggggcaggaa
    13201 attaagcgaa agatgctctg ccccgggttt gcagcgaccg gaacggtcac gccacccttg
    13261 tcgcgctgtg gcctgcggcg gctcccgaaa ctcaaccgat gatgggggct gaaaaccaga
    13321 gagaggagaa agcaggatga gtacccgaaa tatccacgtt aacaccgcga cgtacacgct
    13381 tcttgttgcc gggaagaaaa aaaacaccgg ggaagagtgg gacgtcctgg aattcagcag
    13441 cctgactgag ctgaaaaaat atcgcaaaag ccacccggaa aagatggcct tcagctatag
    13501 ctacgcgctc agccggggtg tggatacgca gttccgccat atcaacattg cggaagccga
    13561 tcacttcaaa cagttcctgc gtcagattaa gcgtaccggc ctcgatatcc gggcgatctg
    13621 ctgaccggat gtgatgaata tccctcctga ttgttgcaat cttgttaatg gagaccggga
    13681 gggatgatat taagcggaaa aattttttat taaatttatt ctttcataaa ggagtagctg
    13741 ttatgcgatt agcttcccgt tttggtcggt ataattccat tcgccgtgag cgtcctttaa
    13801 cggatgatga attaatgcag tttgtgcctt cggtattttc cggtgataaa catgaatccc
    13861 ggagtgagcg ttatacatat attccaacaa tcaatatcat caataagtta cgtgaagaag
    13921 gtttccagcc gttctttgca tgtcagagtc gggttcggga tttgggacgc cgcgaataca
    13981 gtaaacatat gttacgtctt cgcagggaag ggcatattaa cgggcaggaa gttcctgaaa
    14041 ttatcctgct taattcacat gatggttcat ccagttatca gatgatccca ggaatttttc
    14101 gttttgtctg cacaaatggt ctggtgtgcg gaaataattt tggcgaaatc cgcgttccac
    14161 ataaaggcga tattgtcggg caggttattg agggagcgta tgaagtgccc ggtgtctttg
    14221 ataaggtcac tgaaaatatg gaggcgatga aagaaataca tcttaacagt gacgagcaac
    14281 atttatttgg cagagctgca ctgatggtca ggtatgaaga tgaaaataaa acaccagtga
    14341 cgcctgaaca aataattact ccccgtcgtt tggaggataa acagaacgat ctctggacaa
    14401 cctggcagcg ggttcaggag aatatgataa aaggtggact gtcggggcga agtgcctccg
    14461 gtaaaaatac caggacaaga ggcattacgg gtattgatgg tgatatccga atcaacaaag
    14521 cattatggat gattgccgaa cagttcaggg agtggaagtc atgataatat aagtaaagcc
    14581 ccgaaaattt tttcggggct ttacttatat tatcattatg cggcattgat atgtcgtctg
    14641 agatgcctca atttcatcaa ctggcttttc ggtagtctgt aaatattgtg cccgcgaaaa
    14701 atggtctggc aggaggctgc gcagttaccg gatatatata agatgggttc ccgctttgcg
    14761 gggcttcggc cagtcagcat tatcactcat atgatatttt tgtgtgtggc cttccatgcc
    14821 gcgtttgcgg cataaccggt tttttaaaat gtatatcaaa ttaattcttc tgcgagctgt
    14881 tcatttttga aagcggtttt tcagatgcac gaagctggat ccccttgctg ttttttatcc
    14941 cgctgtaaat ccggtaaaca tctgatgcat aggcaagacg ccgctggttc tggcgttcgg
    15001 ttttcctgaa tcctgcgttg tatgcaccaa cagcctccca ggagactccc cattttttaa
    15061 aggctatagc cagataataa gcaccggtat aaatgttcag acagggatct gctgtcagat
    15121 gttccggttt aattccgtag cgggccagtt cgttaaaatg ctgggaatct acctgcataa
    15181 gtccactgcc atatcccgta accggattaa taccgatggc attaacccgg ttacgggatt
    15241 ctttccatga tattgctctc agcaagtcag gggcaatttt ataatctctg ccagccagat
    15301 caaagcaatc accagccttg caggtcacat ttataaacat caggcagatc gtcagaagcc
    15361 attttctcat tttctcacct ctggtgacgt catcaggaaa taatttaacc cactccacaa
    15421 aaaggctcaa caggttggtg gttctcacca ccaaaagcac cacaccccac gcaaaaacaa
    15481 gtttttgctg atttgcgttt taaatcatgt aattaataaa tgctatttat atttaataat
    15541 tctgaattat taaatagaga gtcgttggcg atcctgttac gaccctatag cgactcttat
    15601 taggatcagt actgacaatt ttcatgaaaa acattgatat atattaggga cgcccctagc
    15661 gccggatcgc atttttttat aggatgatcc tagaggcgct gctaggagtg tctttcttac
    15721 ttgtaaacac tatatgcata tttatagtga aaagttcatt gcttaataat gaaaggtttt
    15781 atcttatggc cagagtaatt ttgtatatca gtaatgatgt ctatgataaa gtaaatgcga
    15841 tagttgaaca gcgtcgtcag gagggcgcaa gagataaaga tataagcgta tcgggaactg
    15901 cttcaatgct tcttgaacta gggcttcgtg tatatgaagc tcagatggag cgtaaaaagt
    15961 ccgcgtttaa tcagattgag tttaataaat tacttcttga atgcgttgta aaaacacaat
    16021 catcagtagc gaaaattttg ggtattgagt ctctaagtcc ccatatctcc gggaatccaa
    16081 agtttgaata tgccaatatg gttgaagata tccgggagaa ggtgtcatct gagatggaac
    16141 gattttttcc aaaaaatgat gatgaataaa aaagaattaa aagttgattt acttcaaata
    16201 acagagtttt gatctggttc aatttgatga caattaacca cataaagggt atattaatta
    16261 tgtggttaat gccacgttaa aaatttagtt taaatcgcca atgcagggag tacccgctcc
    16321 ctgcattgac tgtccataga atcctttgag gaggttccta tgtgtgcaat ggatcgtaga
    16381 gaaagagcac tcattcgtca attacatttc cctaacgact taagagcgaa catttcatta
    16441 ttgaaatatc ccgtatgtat acgttttgaa gacggttctt ttataggaac aaatcacagc
    16501 ttcgagcgat ttattaaatt atcatattgt tcatgtgatg aatggtttga gcatttgact
    16561 ttagaatcca agttacaact atcaagatct gagatggatt catatttttc aacttatggt
    16621 gtaagttata ttaataatgt ttttattagt gctatgtcat ggagcatggt tatcgaaaca
    16681 ctcaatacaa acgaaggttg ttttttcgtc tggcgattta ttgagtcaaa tgacaaaaat
    16741 atttcaacat ctttgatttc taaatctaat gatgggttga tgtttttttt ctggggaata
    16801 tgtagatatt gaaccctatc ttgtagggtt tagtcattat tattctgcag cagtgacaaa
    16861 aattagtata tctaaatcca agaag
//
LOCUS       DAZGSP010000111        16851 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZGSP010000111 DAZGSP010000000
VERSION     DAZGSP010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.112"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1134)
                     /locus_tag="ACHK74_004067"
     CDS             complement(<1..1134)
                     /locus_tag="ACHK74_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0222797.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHRCDRPSIKRDLVDYNIINELLFNCSKIQPVENKKDANETLE"
     gene            complement(1192..1343)
                     /gene="ylcJ"
                     /locus_tag="ACHK74_004068"
                     /pseudo
     CDS             complement(1192..1343)
                     /gene="ylcJ"
                     /locus_tag="ACHK74_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299444.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YlcJ"
     gene            complement(1392..1724)
                     /gene="emrE"
                     /locus_tag="ACHK74_004069"
     CDS             complement(1392..1724)
                     /gene="emrE"
                     /locus_tag="ACHK74_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFP0222798.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGLFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSTPH"
     gene            complement(1792..2094)
                     /locus_tag="ACHK74_004070"
     CDS             complement(1792..2094)
                     /locus_tag="ACHK74_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFP0222799.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            complement(2091..2792)
                     /locus_tag="ACHK74_004071"
     CDS             complement(2091..2792)
                     /locus_tag="ACHK74_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP0222800.1"
                     /translation="MKNIAAQMVNFDCEQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVNAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(2789..3808)
                     /locus_tag="ACHK74_004072"
     CDS             complement(2789..3808)
                     /locus_tag="ACHK74_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP0222801.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNTGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTAGKPKLDLTNTDWI
                     YGVEL"
     gene            complement(3805..4344)
                     /locus_tag="ACHK74_004073"
     CDS             complement(3805..4344)
                     /locus_tag="ACHK74_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFP0222802.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(4414..4644)
                     /locus_tag="ACHK74_004074"
     CDS             complement(4414..4644)
                     /locus_tag="ACHK74_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0222803.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            4749..5438
                     /locus_tag="ACHK74_004075"
     CDS             4749..5438
                     /locus_tag="ACHK74_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFP0222804.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            6034..6240
                     /locus_tag="ACHK74_004076"
     CDS             6034..6240
                     /locus_tag="ACHK74_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFP0222805.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            6316..6612
                     /locus_tag="ACHK74_004077"
     CDS             6316..6612
                     /locus_tag="ACHK74_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP0222806.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIQYMVETIAHHQVDIDSEV"
     gene            6618..7403
                     /gene="bet"
                     /locus_tag="ACHK74_004078"
     CDS             6618..7403
                     /gene="bet"
                     /locus_tag="ACHK74_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFP0222807.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            7400..8080
                     /locus_tag="ACHK74_004079"
     CDS             7400..8080
                     /locus_tag="ACHK74_004079"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP0222808.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMANFDEMVPEFIEKMDVALAEIG
                     FVFGEQWR"
     gene            8077..8238
                     /locus_tag="ACHK74_004080"
     CDS             8077..8238
                     /locus_tag="ACHK74_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HFP0222809.1"
                     /translation="MKHPHDNIRVGAITFIYSVTKRGWAFPGLSVIRNPLKAQRLAEA
                     INNKRGLYD"
     gene            8231..8788
                     /locus_tag="ACHK74_004081"
     CDS             8231..8788
                     /locus_tag="ACHK74_004081"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFP0222810.1"
                     /translation="MTDFTGSNTPAEHRDSWRTPPEIFAALNAEFVFQLDAAASEKNR
                     LCRLFISQEQNTLTTSWPEAMGYASGYVWLNPPYSNISPFVKKAATENKFSSVGCVML
                     LPADTSVGWFHEAIQTASEVRFITAGRLAFINPLTEKPVSGNNKGSMLIIWHPYPRTH
                     CHFTTVDRGELMAFGSRILARREAA"
     gene            8865..9080
                     /locus_tag="ACHK74_004082"
     CDS             8865..9080
                     /locus_tag="ACHK74_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP0222811.1"
                     /translation="MTHQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            9179..9397
                     /locus_tag="ACHK74_004083"
     CDS             9179..9397
                     /locus_tag="ACHK74_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP0222812.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRQNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQWGL"
     gene            9445..9723
                     /locus_tag="ACHK74_004084"
     CDS             9445..9723
                     /locus_tag="ACHK74_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0222813.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            9695..9760
                     /locus_tag="ACHK74_004085"
                     /pseudo
     CDS             9695..9760
                     /locus_tag="ACHK74_004085"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            9922..11085
                     /gene="intD"
                     /locus_tag="ACHK74_004086"
                     /pseudo
     CDS             9922..11085
                     /gene="intD"
                     /locus_tag="ACHK74_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(11101..11177)
                     /locus_tag="ACHK74_004087"
     tRNA            complement(11101..11177)
                     /locus_tag="ACHK74_004087"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11141..11143),aa:Arg,seq:tct)
     gene            11420..12052
                     /gene="fimZ"
                     /locus_tag="ACHK74_004088"
     CDS             11420..12052
                     /gene="fimZ"
                     /locus_tag="ACHK74_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFP0222814.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(12055..12570)
                     /gene="sfmF"
                     /locus_tag="ACHK74_004089"
     CDS             complement(12055..12570)
                     /gene="sfmF"
                     /locus_tag="ACHK74_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFP0222815.1"
                     /translation="MRRALFSCFCGLLWISSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(12581..13588)
                     /gene="fimH"
                     /locus_tag="ACHK74_004090"
     CDS             complement(12581..13588)
                     /gene="fimH"
                     /locus_tag="ACHK74_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFP0222816.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYILMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(13601..16209)
                     /gene="sfmD"
                     /locus_tag="ACHK74_004091"
                     /pseudo
     CDS             complement(13601..16209)
                     /gene="sfmD"
                     /locus_tag="ACHK74_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            complement(16240..>16851)
                     /gene="fimC"
                     /locus_tag="ACHK74_004092"
     CDS             complement(16240..>16851)
                     /gene="fimC"
                     /locus_tag="ACHK74_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFP0222817.1"
                     /translation="ALGATRIIYPADAKQTAVWIRNSHTNERFLVNSWIENSSGVKEK
                     SFIITPPLFVSEPKSENTLRIIYTGPPLAADRESLFWMNVKTIPSVDKNALNGRNVLQ
                     LAILSRMKLFLRPIQLQELPAEAPDTLKFSRSGNYINVHNPSPFYVTLVNLQVGSQKL
                     GNAMAAPRVSSQIPLPSGVQGKLKFQTVNDYGSVTPVREVNLN"
BASE COUNT         4648 a         4032 c         3772 g         4399 t
ORIGIN      
        1 ctctaaagtt tcattagcat ctttcttgtt ttcaactggt tgaattttgc tacaattaaa
       61 aagcaattca ttaatgatat tataatcaac caaatctctt tttattgatg gcctgtcaca
      121 tcgatgtaat cttctcatcg gacaaacata atagccatgc aaacttccag ataccgcatg
      181 aacaatcatg gtattaccac aagcctcaca cttcataact gttcgaagta gatttattag
      241 cataggattc ttgctactat tgctaatacc aaaaggtgcc aaccgaattt cctgtacagc
      301 gtaaaacaaa tcatctgata tgactctggg ataatagcca gcgatttcac ttatcccttt
      361 ccctcttgca cgatatgaag gtacgcatat acctatcaga gctttattcg ctaataattt
      421 ttcaattaca gaaggtcccc atgcactttc ttttcctgag aaattcttta cagcatgatc
      481 atttaaatac ttggctattg cattcaatga gcgcctttcc atcctgagtt taaaaattag
      541 ctcaatagtt ttcaccctgt cggggtctgg aacaaaagcc gttcttttgt catctaagga
      601 gagccatctc ggacaagacg ccgtcataat cgtacctgat tccagtgcat cctgccgttt
      661 tttcttccat gataatttaa cccgacttga ctttatctcg ctttcttcat ttgccctttg
      721 tgctataagt atggctttta ttaatgaata tggctcattc aaagagtcaa tattatagac
      781 tgtattgtcg caaagagtta taacatcaat accgtgattc aaaattaatt tcagacgttc
      841 aatcgcttca ccgacttttt ctcttgaaag tctgtccaga ctttcaacta acaatgtagt
      901 tcctggcaat atataaccat gctctatagc atctaaaaat tccgaaaaag ctcctgattg
      961 tgcatgcttt cctttgaatg cacttaatcc taaatcttca tatgttatgg tatcaagata
     1021 ataatcacta tttaccttta accattcagc aataagtctt ctctgtcggt ttaatgagtc
     1081 accagacatc tgacctggtg atgaaaatcg catatatgct atggcttttt tcatggtgac
     1141 acctgctaac gtatgctttt ataaacctta gtggtgggat ataatttttg tttaattttt
     1201 atttaaaaaa acaattaagg tcacattatc ttgaatatac aacaataatc gtattgcaat
     1261 tttcttacgc cataatcttg aaagcacaaa agaacacata aaaaaaataa agacattaac
     1321 aaaaagcata aaacgaggct catataaata taagagcctc catattttag tcgtttagaa
     1381 acaaattatt tttaatgtgg tgtgcttcgt gacaatagat taataaccaa cacaccggca
     1441 caaatcaaca tcatgcctat aatggctggc aggtccagcc gttggccgaa aagtccccat
     1501 gacagtaagc taatcaggac aataccgact cctgaccaga tagcataagc aatccctgta
     1561 ggaatataag ccagcgtctg agctaataac cagaatgatg cacaataaca aataattgta
     1621 ccaacagatg gccataaccg tgtaaaacct tctgaaaact tcattaaggt tgtaccaatg
     1681 acctctgcaa gtattgcacc accaagataa atataagggt tcatagcata ttctttcctg
     1741 ttcaaactgg agagaattgt actacagttt gaactcaact cacctgtttc atcattgtgt
     1801 acccattgat gttcttttat ataccctcaa tacccgtttc atcgcggcac tctggcgaca
     1861 ctccttaaaa atcagattcg tgctcacctt tccttcccgt tcttctctgg tagcgaaccg
     1921 gtaatacacc gttcgccaga ccttaccatc aacgaccagg attcctgccc gcgccatttt
     1981 agccgcagcc tgatttatgc tggttacggt tgcgcctgtt accgcggcaa cgtcctgtgc
     2041 acagaagctc ttatgcgtcc ccaggtaatg aataattgcc tctttgcccg tcatacactt
     2101 gctcctttca gtccgaactt agctttaatt tctgcgatct tcgccagagc ctgtgcacga
     2161 tttagaggtc taccgcccat aacaggaagt tgttttactg gttcaggtat cgtctcacca
     2221 cggttaattc gcgctgtcat acaggtcagt tcatcggcag ccttgcgccg taattccgcg
     2281 tcagtcagcg cattggcccg catgttctgg tacaagttgg taaccaacca gtaatgcgcg
     2341 ttcgatttcc acggataaga ctctgcatcc ggatacaggc cacgcttccg gcaatactcg
     2401 taaaccatat caaccagctc gctgacgttt ggcagcccgg cgttaacaga tgcttcttcc
     2461 cggcaccagg cgacaaactg cccgggtgat ggcaggaatg gtcgattctg ccgacgggct
     2521 acgcgcattc cagcgttaac ctgttccatt gtggtgatcc cgttttcccg gaaagccaga
     2581 acccactggc ggcggatttc gttcagttca ttctggtcac ggttagccag gctcgccggg
     2641 aaagttgcca gtaactggct gaacacaccg ttgatgatct gcgctacctg ctgtacctgt
     2701 ggcttttcgt cgtactgttc cggcatgttg ttggcgatcc ggcgcatctg ctcacagtca
     2761 aagttaacca tctgtgcggc gatgtttttc atagctccac cccgtaaatc cagtcagtgt
     2821 tcgtcaggtc gagttttggt ttgccggctg tcacgccagc ctgttgcttg tttcggttga
     2881 tttcgagctg ggtccacttg tcgcggagtt tggccggact tagcacgtta ccggaccaga
     2941 agttgtcctg gcatgcccag cggaacagca cgcacatgtc gcggtggtta cgtccgtcac
     3001 gttcacgcat caggcggata tcgttagccc accctgcaaa attcggtttt ctggctgatg
     3061 gcgcgatggt cttcaccatg tcaaacatcc actctgcggc ggtcaggtct tctgctgtcc
     3121 cccacttgct gccgctctga attgcagcat ccggtttcac cacaggaaga tcgttttctg
     3181 gttggtcaga ggattcgcca gaattctcgg acgaaaaagg ttttatattg tcttttgtta
     3241 gtttgtcttt tgtgtttacc tgattcgggt aaacgccttt acctgatttg ggtaaacttt
     3301 ttttacctga ttcaggtaaa tttacctctt tcaggtaaac tttatttttc ttacctgatt
     3361 cgggtaatgt tgaccattca ctgaccacat tattaatgcc ggtattccgc ccgctctgaa
     3421 taaaaatccc acgctttacc agaacacttt ttgcagcaga acacttgtgc ggcaatatcc
     3481 cggttaattc ggaaagttgc tcgttgctaa cccaatccag ttttttatta aagccatatg
     3541 ttttgcgcat gacagccaga aagaccagaa gctggtgctg tgttaatccg gccagcatca
     3601 cagcttccag caactcattt gcaatgcgcg tataaccatc atcgagatct gccacgcgcg
     3661 gctccttttg tgccgcatcc ggcactggaa aattgaatat ctcagcagtg tttgccataa
     3721 ttcctcccgc aatgagtgtg ttacgatttg cacctgaaag tcggttctgt tccagcagac
     3781 cggctttcgc catttctgaa cctgtcatat cgcccccagc atggtagtaa ccatcgccat
     3841 caatggacca gccagatctg ggtccacacg aaacatcgac acaatacctt cactaatttc
     3901 cttcagtttc tggtggcgtg gtgcgttgag aatgacagcc tgttttgcct cactgagttc
     3961 cttttccatt tcagccaacc tagccatgaa gctatcctgc tcaaccaggt aaccgcgata
     4021 ttccagcggt agtaccgcca gaattgccgg ggtcagttca cgcacgttat ttcggtattt
     4081 ttcagaatcg aatttgttat cgaggaagcg gaacagcttc tggcgtgcac ggctgacatc
     4141 atcagggaaa tcgatggtgc cgccgccctg ctcccgatac tcattcacaa tgagtgtggc
     4201 aacgacatcc tgattatcta cagccgacca ggcgcggacg gcatcacgga ttttttcgtg
     4261 gcctggcacc tgttttgttt gagaacgatt tatcaccgca gtcgggctaa atccgctagt
     4321 ctgttggtat gtaagtggtt gcataattga ctcctttagt ttgaattgac tgttaagttg
     4381 attgcttatt gttaaagagc gtgaaatgga aatttaagct gcgttctttt cggtgtgtgg
     4441 aaacaacttc ggaagatccg ggcgaatctg gtatgccttc actactccac cagtagccgt
     4501 aacaatgctg ccgacatgtt caggggatac ctttgctttg ttgtgaagcc acttatagac
     4561 ggcctgctgt gaaacttcgc aagcagcgcc cagtttcttt tgtgaaccaa cgatattgat
     4621 cgctgttttg atagctgggt tcataacaac ctccgtggtt aatttgaatc aagattaaaa
     4681 ctatggttgt ttttagtcaa caaccatttt cgtttgataa aataaaacct tggttgtaca
     4741 tttggactat gaaaacaaca ctctcagaaa gacttaaaga agccagatta gcgcgaggcc
     4801 ttacacaaaa ggcgcttggg gatttggtcg gggttagcca ggctgctatt cagaaaatcg
     4861 aaacagggaa agctaatcaa acaactaaaa tcgtggagat cgcgaacgct ttgggtgtgc
     4921 gcgcagaatg gttatcttct ggcgttggaa atatgtcaga cagtacagtg caaccaatac
     4981 aatcaactgt cagccattcc aaatacttca agattgacgt tcttgatata gaagtcagtg
     5041 ctgggccggg agtcatcaac cgtgagtttg tagaagttct acgctcggtt gagtactcgt
     5101 ttgacgatgc tcgtcacatg ttcgatggta ggaaggcgga aaatatccgc atcattaacg
     5161 tgcgtggtga cagcatgtca ggaacgatcg aaccaggtga tctgctgttc gttgatatca
     5221 cagttaaatc tttcgacggt gatggtatct atgcgtttct gtacgacgac acagcccatg
     5281 taaagcgcct gcaaatgatg aaggataagc tgctggtcat ctctgataac aaaagctact
     5341 caccgtggga cccgatcgag aaagacgaga tgaaccgggt gttcatcttc ggtaaggtta
     5401 ttgggagcat gccgcagaca tataggaagc atgggtagta ccaattaaaa attatcaact
     5461 gggcattgtg ctcattcagt aaagaactaa ttcctatctt tgctctaggt agtaatatta
     5521 agccaccgca ataatatctt tacctaacgg cgtaagaatc ccggtcaccg tgccgggttt
     5581 tcttttgccc tcccctcatc acacacaccg ttaaaaaaac caccataacc tcgcttcagt
     5641 tatcgctatg cgattcaagt cacaaaataa atccatccta aatacaacca gttatatcta
     5701 aaacaaccaa taaaacaact tttgttgttg acgataaaac aactatagtt ttaaataagt
     5761 tcatcgcaac aacacaacga tacggcaact acctgattca ccgttgcgat gaccgcttag
     5821 atccgcagtt tgaatttcag caggcttcgg ggagtgcgag gggtgaaacg gacgcgtgaa
     5881 cgtcggtgtg accagctgaa attaactcaa catttcatac cttagtcgct tcaacgaggc
     5941 ggcttagtta tgacaaccgg cggccatcca ccgcctgaat acgcgcagaa gtctctatat
     6001 gttcagcagc ccagcttacg ggcaggagtt tttatggttc atcaacatta tggaacgcag
     6061 accgttaatc gaggtgcggt catgccagga atgctggtca aacacaaaga tggtacctgg
     6121 actgcatcag ctaatttacg cggacggctt tatctgcatc gcggcatcga gcgcacttat
     6181 acccgtgatt tgctcgtgga agtttttctc gacggacgcg gtaacggcct gaatcactaa
     6241 tcccctttcc tgttttccta atcagcctgg catttcgcgg gcgatatttt cacagccatt
     6301 ttcaggaggt cagccatgaa cgcttattac attcaggatc gtcttgaggc tcagagctgg
     6361 gcgcgtcact accagcagat cgcccgtgaa gagaaagagg cagaactggc agacgacatg
     6421 gaaaaaggcc tgccccagca cctgtttgaa tcgctatgca tcgatcattt gcaacgccac
     6481 ggggccagca aaaaagccat tacccgtgcg tttgatgacg atgttgagtt tcaggagcgc
     6541 atggcagaac acatccagta catggttgaa accattgctc accaccaggt tgatattgat
     6601 tcagaggtat aaaacggatg agtacagcac tcgcaacgct ggcagggaag ctggctgaac
     6661 gtgtcggcat ggattctgtc gacccacagg aactgatcac cactcttcgc cagacggcat
     6721 ttaaaggtga tgccagcgat gcgcagttca tcgcattgtt gatcgtcgcc aaccagtacg
     6781 gccttaatcc gtggacgaaa gaaatttacg ccttccctga taagcagaac ggcatcgttc
     6841 cggtggtggg cgttgatggc tggtcccgca tcatcaatga aaaccagcag tttgatggca
     6901 tggactttga gcaggacaat gaatcctgta catgccggat ttaccgcaag gaccgtaatc
     6961 atccgatctg cgttaccgaa tggatggatg aatgccgccg cgaaccattc aaaactcgcg
     7021 aaggcagaga aatcacgggg ccgtggcagt cgcatcccaa acggatgtta cggcataaag
     7081 ccatgattca gtgtgcccgt ctggccttcg gatttgctgg tatctatgac aaggatgaag
     7141 ccgagcgcat tgtcgaaaat actgcataca ctgcagaacg tcagccagaa cgcgacatca
     7201 ctccggttaa cgatgaaacc atgcaggaga ttaacactct gctgatcgcc ctggataaaa
     7261 catgggatga cgacttattg ccgctctgtt cccagatatt tcgccgcgac attcgcgcat
     7321 cgtcagaact gacacaggcc gaagcagtga aagctcttgg attcctgaaa cagaaagcca
     7381 ctgagcagaa ggtggcagca tgacaccgga cattatcctg cagcgtaccg ggatcgacgt
     7441 gagagctgtc gaacaggggg atgatgcatg gcacaaatta cggctcggcg tcatcaccgc
     7501 ttcagaagtt cacaacgtga tagcaaagcc ccgctcagga aagaagtggc ctgacatgaa
     7561 aatgtcctac ttccacaccc tgctggctga ggtttgcacc ggtgtggctc cggaagttaa
     7621 tgctaaggcg ctggcctggg gaaaacagta cgagaacgac gccagaaccc tgtttgaatt
     7681 cacttccggc gtgaatgtta ctgaatcccc gatcatctat cgcgacgaaa gtatgcgcac
     7741 cgcctgctct cccgatggtt tatgcagtga cggcaacggc cttgaactga aatgcccgtt
     7801 tacctcccgg gatttcatga agttccggct cggtggtttc gaggccataa aatcggctta
     7861 catggcccag gtgcagtaca gcatgtgggt gacgcgaaaa gatgcctggt actttgccaa
     7921 ctatgacccg cgtatgaagc gtgaaggcct gcattatgtc gtgattgagc ggaatgaaaa
     7981 gtacatggcg aattttgacg agatggtgcc ggagttcatc gaaaaaatgg acgtggcact
     8041 ggctgaaatt ggttttgtat ttggggagca atggcgatga agcatcctca cgataatatc
     8101 cgggtaggcg cgatcacttt catctactcc gttacaaagc gaggctgggc atttcccggc
     8161 ctttctgtta tcagaaatcc actgaaagca cagcggctgg ctgaggcgat aaataataaa
     8221 cgggggctgt atgactgatt tcaccggaag caatactcct gccgaacatc gcgacagctg
     8281 gcgcacacca ccagagattt ttgctgcgct taatgcagag ttcgtttttc aacttgatgc
     8341 tgccgccagc gaaaaaaacc gactatgtcg gctttttatc tcacaggagc agaacacatt
     8401 aaccacttca tggcctgaag caatgggata tgcctctggt tatgtctggt tgaatccacc
     8461 atacagcaat atttcccctt ttgtgaaaaa ggcagccact gaaaacaaat tcagtagtgt
     8521 gggatgtgta atgttattgc ctgctgacac atctgtcgga tggtttcatg aagcgataca
     8581 aaccgccagt gaggtcagat tcatcacggc aggacgactg gcatttatta acccactcac
     8641 tgagaaaccc gtcagtggaa ataataaagg ctcgatgctc attatctggc acccataccc
     8701 ccgtacacac tgccacttta cgaccgttga tcgtggagag ttgatggcgt tcggctcaag
     8761 gattcttgcc cgtcgggagg ctgcatgaca accacggaat gcatttttct ggcagcgggc
     8821 ttcatattct gtgtgcttat gcttgccgac atgggacttg ttcaatgaca catcagcagg
     8881 aaaacgccct tcgcagcatt gcccgtcagg ctaattctga aatcaaaaaa gccagacagc
     8941 agtttccgga taaaaacgtc gatgacattt gccgtagcgt acttaagaag caccgcgaaa
     9001 cggtaacgct gatgggattc acaccgactc atttaagcct ggcgatcggc atgttaaacg
     9061 gcgtctttaa ggaacggtga acatgaaaag caaaatcatc agggagctac aggctccttt
     9121 tttattgttc gcattcatcc tcaagcgtat taaccaacaa ttcagggatt aatggaagat
     9181 ggcagacatc attgattcag catcagaaat cgaagaatta cagcgcaata cagcaataaa
     9241 aatgcgtcgt cagaactacc agactgtatc cgcaactcat tgttgtgagt gtggcgatcc
     9301 gatagatgag cgaagacgcc tggctgttca gggttgtcgg acttgtgcaa gttgccagga
     9361 ggagatcgaa cttaagaaca aacaatgggg actgtgatgg cctcaaagca gcaaatttca
     9421 acatcgtcca actgaggtgt aaaaatgttc agaatcattt ttcctaacac ctggtacgtc
     9481 gaccaccacg gcactccctg caaaatcctg cgttctaccc acaacaaagt tcactacatc
     9541 cgaaaaggca gaacatgtat cgccagcatg ttccgcttta atcatgactt tgaacctgtg
     9601 aataaagctg atgcagatcg gatagcagaa gagatcgaaa cggcagaaca cattaagaag
     9661 ttacgtgaca tgcgttcaaa aagcagaggt aaccatggaa tcatacagcc tcacactcga
     9721 tgaggcctgt cagtttctta agatatccag accaaccgcc accaactgga tacgaacagg
     9781 ccgcctacag gcaacacgta aagatccaac caagccaaaa tctccttacc tcacaacacg
     9841 gcaagcctgc attgcggcgc ttcagtctcc gctgcatact gtccaggtga gcgcgggtga
     9901 tggcataaca gaggaaagaa aatgtcactc ttccgcagaa atgaaatatg gtatgcctcg
     9961 tattcgctcc cgggcgggaa acgaattaag gaatctcttg gcacaaagga caagcggcaa
    10021 gctcaggagt tgcacgacaa gcgaaaagca gaactctggc gagtagaaaa gctaggggat
    10081 ttacctgatg tcacttttga agaggcctgc ctaagatggc ttgaggaaaa agctgataaa
    10141 aaatctctcg attcagataa aagccggatt gagttctggc ttgaacattt tgagggtata
    10201 aggcttaaag atatctcgga ggcaaagatt tactctgctg taagcagaat gcataacaga
    10261 aagacgaaag aaatatggaa acagaaagtt caggccgcca tcaggaaagg taaagaaccg
    10321 cctgtttatg aaccaaagcc agtatcaact cagacaaagg caaagcatct tgccatgata
    10381 aaggccattc tccgtgctgc agaacgcgac tggaagtggc tggaaaaagc gcctgtcatc
    10441 aagataccag cggtcagaaa caagcgagtc agatggctgg aaaaggagga agcaaaacgc
    10501 cttattgatg agtgccccga accactgaaa tctgtcgtca agtttgcgct ggcaactggt
    10561 ctgagaaagt cgaacatcat aaatctggaa tgacaacaaa tcgacatgca gcgacgagtt
    10621 gcctgggtga atccagaaga gagcaaatca aaccgcgcca ttggtgtggc gctgaacgat
    10681 accgcctgta aagtgttgcg tgatcaaata ggcaagcatc acaaatgggt gtttgtacat
    10741 accaaggcgg ctaagcgagc agatggaaca tcaacgcctg cggtcaggaa gatgcgcatc
    10801 gacagcaaga catcatggct atcagcttgt cgtcgtgcag gaattgaaga tttccgtttc
    10861 catgacctca gacacacctg ggcaagctgg ctgattcagt caggcgtccc attatcagtg
    10921 cttcaggaaa tgggcggatg ggagtccata gaaatggttc gtaggtatgc tcaccttgcg
    10981 cctaatcatt tgacagagca tgcgaggaaa atagacgaca tttttggtga taatgtccca
    11041 aatatgtccc actctggaat tatggaggat ataaagaagg cgtaactgat tgaattgtaa
    11101 tggcgcgccc tgcaggattc gaacctgcgg cccacgactt agaaggtcgt tgctctatcc
    11161 aactgagcta agggcgcgtt gataccgcaa tgcggtgtaa tcgcgtgaat tatacggtca
    11221 acccttgctg agtcaatggc ttttgatctg gttgctgaac aagtgaacga ccgcgtctga
    11281 ttttctgatt tatttcgcta tagcggcaaa caaacacaca ccgctgcgcg tctgaatcaa
    11341 gaaaacccgt attttcatgt atcaaagtac aatttaccgc cctaacggaa aattgtccgc
    11401 tcctatgaga ctggtaacta tgaaaccaac gtcggtgatc attatggata ctcatcctat
    11461 catcagaatg tctattgaag ttctgttgca aaaaaacagt gaattgcaga ttgtcctgaa
    11521 aacggatgat taccgcataa ccatcgatta tctccgaacc cgtcctgttg atttaatcat
    11581 tatggatata gacttgcccg gaacagacgg ttttaccttc ctgaaaagga tcaaacaaat
    11641 ccagagcaca gtgaaagtgt tatttttatc atcgaaatca gaatgctttt atgctggcag
    11701 agcgatacaa gctggtgcta acggttttgt cagtaaatgc aatgatcaga atgatatttt
    11761 tcatgccgtt cagatgatcc tctccggata cacgtttttt cctagcgaaa cgcttaacta
    11821 tataaaaagc aataaatgta gtacgaatag ttcaacggtc actgtgctat ctaatcgtga
    11881 agtgaccata ttacgttatc tggttagcgg attatctaat aaagaaattg ccgataagtt
    11941 attacttagc aataaaacag ttagtgcgca taaatctaat atttatggca agctaggttt
    12001 gcattcaatt gtagagctta tcgactacgc caaattatac gaattaatat aatattaatt
    12061 ataattgatc ataaatatcg catccgcttt cgccacacct ggccgaacac cgctggctaa
    12121 cgctcgataa ttggcaaaaa agtttagtgt gacattgcca tttgcatcta cttcctcagt
    12181 cgggctcgcc tcccccagtg cgagccggga gcgatcgcta ttacgtaatt cgatggcgac
    12241 ggtttgtgcc attgcgggat catccagagc cagcaggttg gtatcggatg ccggcgttcc
    12301 cgtaaataaa atcgcaactg aacccggagg acatccctcc agccgcaggc taaaagggac
    12361 gagtgccgtg gtatcgccag cgttcagtaa ttgtgtcgta ggccatctgc ctaaatctac
    12421 cgtcttatca atatccgcag tgtttacggt acaggagaaa tcaacaacgt taccgtgcaa
    12481 attgatatta atcgttccca aagggtcagc tgcccatcca ctggaaatcc acagtaaccc
    12541 gcagaaacag ctaaagagtg ctcttctcat tctccgtacc tcattgataa tcgacgcgta
    12601 aataccccag cgcgctaaac ggcccttcgg tcggtttttg accggtaata ctgataggcc
    12661 aggcgcgaag tgtgacattg gctgccgcag ctgcatccag acggaaagga ataacgctat
    12721 tgagatcgtt aggcgtgatc ggcgtatcgt tctgatcggc gacaataaaa cctaaatcct
    12781 gattgtccga caccatcgcc tgaccagaaa cggcactggc ttccagacgc attgttaaat
    12841 aagcctgcgc agcaacattc gtacatttga ccgcaatgct cttggtttgc ggcatgacac
    12901 cagcaggtcg attacccggc cctgccgcac taaataacga tgcgccgata tcaccaaaat
    12961 caaattcaac aatctgcccg gcatttaatt cgcagttttg cggtacttca acccggccac
    13021 caaaactaat ggtataaaca ggtgtaacca acggatcgta aggcgttgat gtcacataca
    13081 cggtaaacat cacctgtctg gggatctcca ccatgttaat aaagggacga ataaccttta
    13141 aacgaaatat taaacgagaa tccatcaccc cgaatggaag gttttgcgaa acgttaggat
    13201 ctgttcccat cagaatataa ttttgcgggg ggaactgaat atccatcaca ctatcaacca
    13261 gactcatcgc acctaataga taatcattta attgcatata tttataatta tcgatagttt
    13321 cctgaacaat aaagttggtg acataactac ggtatgtata attcaccagc gtaccgggtg
    13381 gacaaattgc tgagacacct acccagccgg atttttccgg taaaacaaca atctgccctg
    13441 gctggttatt ggtgctatta aagatatttg agagatcata gtgttcatct tctgctacgc
    13501 cagtactatt tgcacaaaca gtagcccagg atatgtttgc cagacacaaa catgccatcg
    13561 ccaataataa tctgcagatt atcttcattg ataatttcct ttatgagcca ggatgtgtgc
    13621 aaactgccga aataacagtg acggcttgct gtaagctttg cttcggcaat acatagtgcg
    13681 caatgcagcg tgagttcgcg ccgtctcccc actgaaccag taattcacca gacaatggcg
    13741 caccacttaa ataaacttgc ccgtcatcac ccacatactg gttattccga tactgttttc
    13801 ccgtaccagt gatccaaacg gcaccggttt tccgccctgg gtaacggtaa tgagcgcccg
    13861 cacgcctatg cgggtatcaa aattggcacg aaccaacgcg ccttgcgtcg gcacaacgct
    13921 gctaatattt ttttcaacat cgatggaatt ccccatcgta ttggtatcaa gcgcgatacg
    13981 gttataccga taaaccgtgg catacggcat caccgcatag ccgcgccagt cggttaaaat
    14041 gccagtttga ttttcaatgc gtacaccgcc tgcgccaggc gctttaatca aaacattggt
    14101 atcccctaaa ggctggctca gcgttatgcc attttcatgc ccgaccacac cgccagacag
    14161 ctgccagtta acgtcatgtt gatcgcggtc gtagttatac ccgaccccca gcgtagcgta
    14221 agcggcctgc cagtttgccg tggcgctgcc cgtataccca ttattcgagg tatcaccctg
    14281 gcttacgtga taactcaggt tgtggccttc cagtaaggtc ccacctacac ctgccagcca
    14341 gctattttgc ccgttgctgt tacggttggc gttaaaggag gcataagcgc ggtcgagcgc
    14401 attttcccgg gtgtagcgac gtttggtcag aacattgaaa ggcactgaaa cattaagtcc
    14461 gacaatacgt tcgttatcgg ggatccctac agattcattc cacgaaaatg agagtgaata
    14521 actgatgcca acccagctgc tgatataccc cacctgatac caggtatctg aatccgaagt
    14581 attccagtat ttttgatggg taccagagat ataaagcgag ccaaagtcat taagtgattg
    14641 tgaaatattt aactgcaaac ggtctttacg gctaaagcgt aaattgtggt aattcacgat
    14701 tatcggttca tcccgatgct caccgtcgta atcgtaatca tattcgtacc cctccattcg
    14761 acgatacgca acatcatcaa gggtataaaa accttgtgtc gaatagcggt aacccattaa
    14821 ctgaaaattg gtgccgaagg tgttcatcga tttcgcatag aggaagcgaa tggaatcccc
    14881 ctcatgacga ctgtcgtcgg ctaactgact gcgcgcatgg gtcacatcca gcgacactgc
    14941 cccccagttt ccgagattgc gccccagtcc taataaaaag gcggtgtaat tggcagataa
    15001 ttgcgtcccg ccgtaggcag taaattcctg tggtaaaccg ccgagtgccg tgccctgaaa
    15061 aaagaaaggc gatgattgct gactattacc gctacgaaaa tcgcccgccg tcaggtcaaa
    15121 tttgaaacgc ccttcgcgtt gtaaaattgg cactgttgaa tacgggatag tgtaattctg
    15181 ctgattacca tcgcgctcgt cgatggtgac gtccagatcg ccatttgaag atgtcgggtg
    15241 caaatctgta atttcaaaag cgccggggga aacatagctt tgatagataa taaaaccatt
    15301 ttgccgaatc gttagctggg ccgccgtacg ggcaatccca cgtaccgttg gggcaaaccc
    15361 ttgctggcta tcaggataca tattatcgga agaataaagc cgtacaccac gaaatccaac
    15421 accatcgaaa atatcacttc ctgtattgcc gtctcccatt accagttcac ttttcagcgg
    15481 aataatggcg cgctgtaccc aggtgccaat attattccac tgttctgaat gatatccatt
    15541 tccgcgaaaa tagttccagg aaccattgtt gcgtaaacgc cacgggccaa tattaatccc
    15601 gctgagctca ctaaaaaaat agctatcgtt accattacct ctgttaccgg tgaaattata
    15661 attcaggagt aaagcaggaa taccttcatc ccactcttca ggcggaatgt aaccgtgagc
    15721 gctactcaac aacgttatct gaggaatagt gatgttcagg cgcatcgctg caaaatcaaa
    15781 gttaattgtg gcatcaggta cagctttcag taaattgatg catttattgt tttgctgctg
    15841 ggctaactcg ggaaatgcac tgctattaag gccaattcgt tcaaggagta cctggttaaa
    15901 acagggcatt aacccacctg atttatcacc tgtattttct gttgtcgatt caaatacgat
    15961 atcctgtgaa ccaatgaact catcattacg ccagagatcc acccgataca ctcccgcagg
    16021 ttgatgattg cctctctcaa agcgcgataa atcagcaacg gatgtgccat tctctaataa
    16081 aaatgctggg ttgaaataac tttccgcata gcttaaaaag gagggtaaaa tggcaagaga
    16141 tgaataacaa attccggcca gacaccaggt atacctctgc ggaatatccg tagtagtggg
    16201 tattttcata attagctctg atattgtcag atgattcggt tagtttaagt tcacttctct
    16261 gaccggggtt actgaaccat aatcattaac ggtctgaaat ttcagctttc cctgcactcc
    16321 tgagggtaag ggaatttgtg aactaactct gggtgccgcc atagcattcc ccaacttttg
    16381 gctgcccact tgcaggttaa cgagggtgac ataaaaaggt gatggattat gaacattgat
    16441 atagttaccg gatcgcgaaa acttgagtgt gtccggcgct tctgcgggta attcttgtaa
    16501 ttgaattgga cggagaaata atttcatgcg cgataaaatc gccagttgca aaacattcct
    16561 gccgttcaat gcatttttat ctaccgaagg gatcgtctta acattcatcc agaacagaga
    16621 ctcacgatct gctgccagcg gtggaccggt gtaaataata cgcaaagtat tttcgctttt
    16681 gggttcacta acaaacagtg gcggtgtaat gatgaatgac ttttctttta caccgctgct
    16741 gttttcaatc cacgaattga ccagaaaacg ctcattggta tggctatttc taatccatac
    16801 cgcagtctgt ttagcatcag cgggataaat aatacgcgtg gcacctaatg c
//
LOCUS       DAZGSP010000112        16627 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZGSP010000112 DAZGSP010000000
VERSION     DAZGSP010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.113"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(22..174)
                     /locus_tag="ACHK74_004093"
     CDS             complement(22..174)
                     /locus_tag="ACHK74_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0222818.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(439..1173)
                     /gene="cysH"
                     /locus_tag="ACHK74_004094"
     CDS             complement(439..1173)
                     /gene="cysH"
                     /locus_tag="ACHK74_004094"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP0222819.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1247..2959)
                     /gene="cysI"
                     /locus_tag="ACHK74_004095"
     CDS             complement(1247..2959)
                     /gene="cysI"
                     /locus_tag="ACHK74_004095"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFP0222820.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2959..4758)
                     /gene="cysJ"
                     /locus_tag="ACHK74_004096"
     CDS             complement(2959..4758)
                     /gene="cysJ"
                     /locus_tag="ACHK74_004096"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFP0222821.1"
                     /translation="MTTQVPPSALLPLNPEQLVRLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5077..5439
                     /gene="queD"
                     /locus_tag="ACHK74_004097"
     CDS             5077..5439
                     /gene="queD"
                     /locus_tag="ACHK74_004097"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFP0222822.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5517..6788
                     /gene="ygcN"
                     /locus_tag="ACHK74_004098"
     CDS             5517..6788
                     /gene="ygcN"
                     /locus_tag="ACHK74_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP0222823.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERCITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            6779..7039
                     /gene="ygcO"
                     /locus_tag="ACHK74_004099"
     CDS             6779..7039
                     /gene="ygcO"
                     /locus_tag="ACHK74_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP0222824.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTAERLISACPAGLFSLTPE
                     GDLRIDYRSCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7056..7631
                     /gene="ygcP"
                     /locus_tag="ACHK74_004100"
     CDS             7056..7631
                     /gene="ygcP"
                     /locus_tag="ACHK74_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFP0222825.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7779..8639)
                     /gene="ygcQ"
                     /locus_tag="ACHK74_004101"
     CDS             complement(7779..8639)
                     /gene="ygcQ"
                     /locus_tag="ACHK74_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFP0222826.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8636..9415)
                     /gene="ygcR"
                     /locus_tag="ACHK74_004102"
     CDS             complement(8636..9415)
                     /gene="ygcR"
                     /locus_tag="ACHK74_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFP0222827.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9393..10802)
                     /gene="ygcS"
                     /locus_tag="ACHK74_004103"
     CDS             complement(9393..10802)
                     /gene="ygcS"
                     /locus_tag="ACHK74_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0222828.1"
                     /translation="MTWRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10824..12278)
                     /gene="ygcU"
                     /locus_tag="ACHK74_004104"
     CDS             complement(10824..12278)
                     /gene="ygcU"
                     /locus_tag="ACHK74_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFP0222829.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12348..13133)
                     /gene="ygcW"
                     /locus_tag="ACHK74_004105"
     CDS             complement(12348..13133)
                     /gene="ygcW"
                     /locus_tag="ACHK74_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0222830.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13451..14728
                     /gene="yqcE"
                     /locus_tag="ACHK74_004106"
     CDS             13451..14728
                     /gene="yqcE"
                     /locus_tag="ACHK74_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFP0222831.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDQAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14755..16233
                     /gene="ygcE"
                     /locus_tag="ACHK74_004107"
     CDS             14755..16233
                     /gene="ygcE"
                     /locus_tag="ACHK74_004107"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFP0222832.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWTWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         3983 a         4363 c         4170 g         4111 t
ORIGIN      
        1 ggttttaccc ccgcgtggtg gctacttctt cgattcgtaa gcgagaactg ccttaaactc
       61 cataccacgt tctctgatac tcaactcaca caacgagtcg cctaccatca gcgtgaatcc
      121 cagtactgta aaacacagca ctatgattgc cacaagggca tattttgtca gcatgtcttg
      181 cctccctgca aagaagagac taacattcga attgtttggg ttcgaacgct ggcctcaggt
      241 tgatagaaat atcgcctggg gcttttgtcc atctggaacc tcgcgaatgc ttaacgccag
      301 acagcctcaa gcacccgacg ccattctata cctgataatt cttccagcgg cgttcacgtt
      361 taaaatcagg aatactgctc gcaaaataaa aacgccccca tcatttatga cagaggcgtt
      421 taatttatcc ggcaatattt acccttcgtg caatccacat tcccttttta agccaaagaa
      481 acgtgtttct tcttccgaca tgccaggttc ccatttacgg gttgtatggg tatcaccgac
      541 cgataaatat ccttcatccc ataatgggtg atatttcagg ccatgttttt gcagatactg
      601 atatatcgtt cggttatccc agtcgataat cggcagcact ttaaatacgc cacgctgaat
      661 tgccagcacc ggtaaattgg cgcgactgcc ggattgttca cggcgcaggc cagcaaacca
      721 ggtttgcgcg ttaagttctt tcagagcccg gttcatcggt tcgactttgt tgatgtcatt
      781 gtacttttca atgccttcaa cgccctgctc ccacagtttt ccgtagcgtg cttcctgcca
      841 ggctgcgctt tcggtagcac ggtacacttt caggttgagc ttgagtttgt ccgttaactc
      901 gtcaataaag cggtaggttt ccgggaacag atagccggtg tcggtgagga tcaccggaat
      961 atccgggtga atctgattca ccagatgcag gctcaccgcc gcctgaatgc caaagctgga
     1021 agaaagtaca tattcaccgg gcagattatc cagcgcccag gcaacgcggc cttcagcgtc
     1081 cagtttttcc agttcggcgt tagtttctgc cagcgccaga atgcgatcta ccttcggcag
     1141 ttcgttcagg gcgtttagat cgagtttgga catagattcc tcactgtttg ccttgcctga
     1201 tgcgacgctc acgcgtctta tcaggcctac aagaccgggc tgatggttaa tcccacaaat
     1261 cgcgcgccgg atcgagcacc gggcgaatga tgcccgcacg caccgtaaag tcgccgaagc
     1321 cttcacccgc ttcgcgctct ttcgcccagc gccctatcag ttcatcaagc gacgccagga
     1381 tttccggctc ggtgatgttt tctttataca tccgtgggat acgtgtccca atgcggttgc
     1441 cgccaagatg cagattgtag cgacccggtg ctttacccac caggcccact tccgccagca
     1501 tcgcgcgacc acaaccgttc gggcagcctg ttacacgcat cacgatatgc tcatcgctga
     1561 caccatgttt cgccattaaa ttatcgatgt tgtcgataaa agacggcagg aaacgctccg
     1621 cttccgccat cgccagcggg caagtcggga atgacacgca ggccatcgag ttttcacgct
     1681 gcggcgtgac ggcattcatt aaaccgctct ctttggcgat cttctcgatc ttcgctttct
     1741 cgctttccgg tacaccggcg atgatcagat tctggttcgc cgtaatgcgg aaatcgcctt
     1801 tgtggatctt cgcgatctcc agcaggccgg ttttcagcgg acgccccgga taatcaagga
     1861 tgcgaccatt ttcgataaac agcgtcaggt gccagttatc atcaatgccc ttaacccagc
     1921 caatacgatc gcctcgtccg gtgaactcat atggacggat cggttcaaat ttgatccccg
     1981 cgcgacgctc cacttccgct ttaaacgtct caacccccac gcgctccagc gtgtatttgg
     2041 ttttggcatt tttacgatcg gttcggttac cccagtcacg ctgagttgtc acgacggctt
     2101 cggccaccgc cagcgtatgc tccagcggca gatagccaaa ctcgctcgcc gtgcgggcgt
     2161 aggttttctt gttgccgtgt tcgatagaaa gcccaccgcc caccagcagg ttgaagccta
     2221 ccagcttgcc gttttcggcg attgccacga agttcatgtc gttggcatgc agatcgatat
     2281 cgttctgcgg cgggatcact accgtggttt tgaacttacg cggcaggtag gtctgaccaa
     2341 ggatcggttc ttcatcagta gtggcgactt tttcctggtc gagccagatc tccgcatacg
     2401 cgcgggtacg aggcaacaga tgctcagaaa tcttcttcgc ccactcgtac gcttccgcgt
     2461 gcagctgcga ctcgtaaggg ttcgaggtgc agagtacgtt acggttcatg tcgttagctg
     2521 tcgccagcgc atcaagaccg accgagtgca gcatctggtg caccggtttg acgttctttt
     2581 tcagaatgcc gtggaactga aacgtctggc ggttggtcag gcgaatgctg ccatagatgg
     2641 tgttttcacc ggcaaatttg tcgatcgcct gccactgttt agtggtaatc accccacccg
     2701 gcagacgaca gcgaagcagc atcgcgtggc gcggctccag cttctgttca gcacgttcgg
     2761 cgcggatgtc gcggtcatcc tgctgataca tgccgtggaa gcggatcagc aggaagttat
     2821 cgcccttaaa gccgccggtc agaccgtcgt ttaaatcttc cgcaatggtg ccgcgcaggt
     2881 agttgctttc aagcttcatg cgctcggcgt ctgtcagttt tccttcgacc actaaaggcc
     2941 ctggatgttt ttcgctcatt agtagacatc tcgctgataa cggcgctcta cgcgcagctc
     3001 acttaaaaat tcatccgccg cttcggtgtc catgccacca aattcggcaa tcacttccag
     3061 aagtgcctgc tcaacgtctt tcgccatgcg attagcgtcg ccgcagacat aaatgtgggc
     3121 accatcattg atccagcgcc acagctccgc gccctgttcg cgcagtttgt cttgtacgta
     3181 aactttttct ttttggtcgc gcgaccaggc aagatcgata cgtgtcagca cgccatcttt
     3241 gacgtagcgc tgccactcca cctggtacag gaagtcttcc gtaaagtgcg gattaccaaa
     3301 gaacagccag tttttacctg gcgcttcgtc ggcggcgcgt tgctgcataa aggcgcggaa
     3361 cggcgcaata ccggtgcctg ggccaatcat aatcaccggg gtttctggat tggctggtag
     3421 gcggaagtta tcgttatgtt cgataaatac gcggacttca ccctcttctt ccacgcggtc
     3481 agcgaggaag ctggaggcac caccggcacg ggcgcggcct tccacgtcgt aacgcaccac
     3541 accaacggtg acgtgtactt cgttctcgac ttccgcctgc gaggaggcaa tggaatagag
     3601 acgcggcgtc agcgggcgca gcagattaat tagcgcttcg gcatcaagct gtgccgggga
     3661 gaaacgcacc atgtcgacaa tcggcgtcgt cgcggcgtaa tgctgtaact tcgctttatc
     3721 gcccaccagc ggcagcagtg tttcgctgcg ggtaagcgtg gcgtaattct caacaatgtt
     3781 ggcggtgttg acggtcagtt cgaagtgcca ctgtagcgct tcgttcagag gcaacgtttt
     3841 gccctcgacg gtgacaggtt catcgccttt cagccacagc agttcgacaa gttctttcac
     3901 cagtgccgga tcgttctgat accagacgcc cagcgcgtca cccggctggt aacgcaggcc
     3961 cgagtcacct aagtcaattt cgatatggcg aacgtctttt tcagagttac gcccggtaat
     4021 tttctggtta acagagaggc tagccaccag cggcgcgtct ttgctgtacg ggctggtgtg
     4081 gatttcattt accgcgccag tagcgacgga ttgcgaaggt gccgcgacag gcgcacgcga
     4141 tttaagcgca tcaaccacgc gggcgcgcca ctcgctggca gcagcctggt attcaacatc
     4201 ggcatcgaca cggtcgagca ggcgttcacc acccagttcc gccagcttgc tgtcaaaatc
     4261 tttcccggac tggcagaaaa attcataaga gctatcgccg aggctaaaca cggcaaatgc
     4321 ggtgttttcc agcttcggcg cttttttgga gaacaggaac ttatgcagcg cgacggcttc
     4381 ttccggcggt tccccttctc cttgcgttga cgtcaccacg ataagcagtt tttcgctggc
     4441 gatttgtttg aatttatagt cgcccgcgtt caccagctta acgttcagtt ttgccgctaa
     4501 taaatcatca cgtaatgctt cagcaacacg gcgcgcattg ccggtttgcg aggcggagat
     4561 aatagttata cccggcattt ctgcggcggg agctggcgtc gctgcaagcg cagcaggctg
     4621 ctgattgagc acgccccaga aatagccaga aacccaggca agctgggtgg gagttaaatc
     4681 ggtcgtggcc gcctgaaggc gtaccagttg ctccgggttc aacggaagca acgcggaagg
     4741 tgggacctgt gtcgtcatgc gtcgttatgt tccagtaagc aaagctgttt ctgcgccctg
     4801 tcagcgccca taaaacagaa gagaaggtaa ggttaacggg gtgaacggtg tggattaaag
     4861 acgggatagc gataactaat aaccaaatcg actaacctgt tttagcaata gtctttaaca
     4921 acaaaataga ttaaccaacc taatgaaaaa caaatgaatt tagccaatca ttaagataaa
     4981 tcagcgattt tgcgcaacaa agtcgtttta gataatgcga aaaaacagcc tttccggtac
     5041 tctacggcgg ttttatcgtc ctgtagagaa attatgatgt ccaccacgtt atttaaagat
     5101 ttcaccttcg aagccgctca ccgcttacca cacgtcccgg aagggcataa atgtggtcgc
     5161 ctgcacgggc attcctttat ggtgcgactg gaaattaccg gggaagtcga tccgcatacg
     5221 ggctggatta tcgatttcgc tgaactgaaa gcagcgttta agccaactta tgagcgcctc
     5281 gatcaccatt atctcaatga tattccaggc ctggaaaacc cgaccagcga agttttagca
     5341 aaatggattt gggatcaggt taaacccgtt gtgccgctgt taagtgcggt gatggtaaaa
     5401 gaaacctgca ccgctggttg tatctatcgc ggcgaatgat aagagtgtgt cggcggtcaa
     5461 tttcccttaa gtaacgctat gttagggtgt tgtgttctgg atatctgggg catgacatgg
     5521 aagacgactg cgacattatt attattggtg ccggtattgc aggcaccgct tgcgcgttac
     5581 gctgcgcgcg agcgggttta tccgttttgt tactggaacg cgctgaaatc cccggcagca
     5641 aaaatctttc cggcgggcgg ttatataccc atgcactcgc ggaactcctc cctcagtttc
     5701 atctgaccgc gcctcttgaa cgatgcatca ctcacgaaag cctttccctg ttaacgccgg
     5761 atggcgcaac gacgttttcc agcttacagc ccggcggtga atcctggagt gtattacgtg
     5821 cacggttcga tccgtggctg gttgccgaag ccgaaaaaga aggtgtcgaa tgcatccctg
     5881 gtgcgacggt ggatgcactg tatgaagaaa acggcagggt gtgtggtgtc atttgtggtg
     5941 acgatattct ccgcgcccgt tatgtggtgc tggcagaagg tgccaacagc gtcctggctg
     6001 agcgtcacgg gttagtgact cgtcctgctg gcgaagcgat ggcgttgggg atcaaagaag
     6061 tgctgtcgct ggaaacatcc gctattgaag aacgttttca tctggagaat aacgaaggcg
     6121 cagcgttgct gttcagcggc gggatctgtg atgacttacc cggcggcgca tttctttata
     6181 ctaatcaaca aacgctctcg ttagggattg tttgcccgct ctcttccctt acgcaaagcc
     6241 gtgttccggc aagcgagctg ctgactcgct ttaaagcgca tccggcagtg cgcccgctta
     6301 tcaaaaacac ggaatcactg gagtatggtg cgcatctggt gccagaaggt ggcttgcaca
     6361 gtatgccggt gcaatacgcc ggtaacggct ggctgctggt gggcgatgcg ttgcgcagtt
     6421 gcgtcaatac cggaatttcc gtgcgcggca tggatatggc gctgactggc gcgcaggcgg
     6481 cggcacaaac gctgataagc gcctgccagc accgcgagcc gcaaaatctg tttccgcttt
     6541 atcatcacaa cgtagagcgc agcctgctgt gggatgttct acagcgttat cagcatgttc
     6601 cggcgctttt gcaacgcccg ggatggtacc gtacgtggcc tgcgttaatg caggatattt
     6661 cccgcgattt atgggatcag ggtgataaac ctgttccacc gctgcgccag ttattctggc
     6721 atcatttacg tcgtcacggc ctgtggcatc tggcgggcga tgttatcagg agtctgcgat
     6781 gtctgtagcc cgtaatctct ggcgcgttgc tgatgcgccg cacattgttc cggctgactc
     6841 cgttgagcgc cagacggcag aacggttgat tagcgcctgt ccggcaggtc ttttttcgct
     6901 cacaccggaa ggtgacttac gtattgacta tcgcagttgc ctggagtgtg gcacctgccg
     6961 tttgctgtgc gacgaatcaa cactacaaca gtggcgctat ccgccttccg gattcggcat
     7021 cacctaccgc tttggataaa taataaggat aatttatgcc cctcttacac ctgctccgcc
     7081 agaatccggt gattgctgcc gttaaagata atgccagcct gcaactggca atcgattctg
     7141 aatgccaatt tatttccgtg ttgtacggca atatctgcac catcagtaat atcgttaaaa
     7201 agattaagaa cgccgggaaa tatgctttta ttcatgttga tttgctggaa ggcgcgtcaa
     7261 ataaagaagt cgttattcag tttttgaaac tggtgaccga ggcggacggc attatcagta
     7321 ccaaagcctc aatgttgaaa gcggcaagag cggaaggttt tttctgtatt catcgcctgt
     7381 ttattgttga ttcaatttcg tttcacaaca ttgataagca agttgcgcaa tcgaatccgg
     7441 attgtattga gatcctgcca ggctgtatgc ccaaagtgct gggctgggtg acagagaaaa
     7501 tccgccaacc gctgattgcc ggtgggctgg tgtgcgatga agaagatgcg cgtaatgcga
     7561 ttaacgcggg tgtcgtggcg ctttccacca cgaataccgg ggtctggacg ttagcgaaaa
     7621 aattactttg acgggataat cgtaaccaat tgaatttggt ttgatttgta ggccgcacgc
     7681 cacatccgac attcagcgcc tgatgcgacg cttgacgcgt cttatcaggc ctacaagtcc
     7741 ccaggccaga accgtaggca ggatcatccg gcaacacgtt attggcaatc tgcgtgaatg
     7801 tttgtcacca gtgcctccag caccactttc cagtcatcaa ccacgccaac atctgcctgt
     7861 gaaaataccg cagcgctggc gtcatgatta atcgccacca caaacttgct attgcgtacg
     7921 cccgccatta acgctgctgc gccagaagcg ccaacaacaa tacacacctc aggtgccagc
     7981 agatgtccgg aaataccaat cactttttca gcatcaacgc caccgttcat cactcgcgcc
     8041 cgactgtagc ccacttcggc tcccagcttt tctgccagca tagcgatttc ctggttatct
     8101 gcctctccgc cctgccccac caccagcacc cgtctggcct cagctaatgg gtcgcgagta
     8161 acatttttca gatcctctgt gctaaccagc cagtccggaa gcgcacccgg gacaatgttc
     8221 agttgttgca ttccagaggg caacgtggcg tttttcgctg ctcccgcctg acgcgccagg
     8281 gaaaggcata gcgggcgttt ttctgtttgc agcgtcgccg ttagcgcatt tccccagtgg
     8341 gatttgcgca cgcttacggt tggaatatct aacgaagtca cctggcagat gcttgcacca
     8401 tgtaaacgcc aggccagccg ggtcgacagt tcatcgccaa acgttcccgg cggaaagaga
     8461 acaacgtccg caggcgttcg ctgccactgt tccaccaacg catccagcac ctgttccgcc
     8521 actacaggct gaggttcaat ctgccagtgc gccagggtac aaccgctgaa atcctgcgcc
     8581 gccagccagc ttgctattgc ggcattctcc tgatttattg tcacaattgc gatattcatg
     8641 gctgcatcct ttggcgtaaa tagtcctgcc ataacttttg agctttttct gctaccgttt
     8701 gcccatcaat cagcgtcgct ccgcgtcgtt gctctgctct tgccagttgc agacactgca
     8761 tcgctggcat ttcggcagca acagttttac ggataatttc tgctttcccc gcagccattc
     8821 gctggcgcat tcccggtact ggcaaggcca cttcaccgca ctgacgcacg gcaatcaccg
     8881 caggcaagcg aacccggcaa caacgcaacc catgttcagt acgctgttcg agggtgataa
     8941 acagcgcgtc gagagtgaaa cgctccacct gggtgaagca gggccagccc agcatttccg
     9001 ccagcaaaaa tggcgtttgc ccattctgcc cttcgctgct ttggcagccg gtaattatca
     9061 gatccagcgg attctgatgc tgccattcgg caatatggcg agcgacaaat tccggcgcaa
     9121 agcgcagatc tgccgccgtc tccagcaata cagcttcctc aaacccaaga gccatgagat
     9181 agcgcagcca gtgcaatgcc cgttcatccc ccatgctcaa cgcggttaaa gacatcggcg
     9241 taccgttttt tctctgcgcc aacagcagcg cggcagcggc ctgttcatca gcaccgagta
     9301 aacttcgcag tagcgaaata tccggtccgc ttttaccctg agccgccgcc tgccactctt
     9361 tttccgccag cattccggca tccggttcgg ctttaaacgc taacagaatg ttcattcgca
     9421 cctcctacat ttcccgccgc caccagcggg agggctttag tttctggtgc ccatagccag
     9481 gtcacaacaa aaccaaccaa caacactgtc gccaggagca ataaggtgac ttgcattccc
     9541 cactgcgcca gcacccacgg cagcaggcca gtacttaccg ccgcgccaag tcgactcatg
     9601 gcggtggcaa aaccgacgcc cagcgagcga atgtcagtag gaaaactttc cgcaggcaaa
     9661 atgcccacca gattactgac tgccgaaatg gtggtgctga agagaacaaa aagcagcagc
     9721 gttaatgaac tgccggaagg caaacaggcc attactacca gcgttgccgc cagcagcaaa
     9781 aaacttccca gcaaaaattt gcgatgtgcc agcaggtgcg tcagaactaa tcccagcagc
     9841 gcgcccacaa ttaacaacgc attaagcatc aggctggcag tcagcgcatc ttccagacca
     9901 atagtctggg cgatagttgg cagccaggta taaatcacaa accatgggat tacgaggcag
     9961 acaaagaaga cgctgttaaa cgccgtgcgc cgccagtaac gcgaagagaa caaggttttg
    10021 atgtgtttat gggtcgccgt taccacttca tcgcccagta aaacatgggg accaaaatag
    10081 cgatgcacga tagcgtgagc ttctgcaaaa cgcccctggc gcagtagcca gcgtggtgat
    10141 tctggcgttc cccagcgtaa taacgtaatc aacaacgcgg gcagagctgc cgatgccagt
    10201 agccagcgcc aggcctccgg gttttcggaa ataaagtgat gtccggcaat acttgccagc
    10261 acatagccta cggtccacac cacgctgaat gcgcccagca aaataccgcg atggcggcgc
    10321 ggggaaaatt cagccagcaa ggtgtgacct actgaataat cgcctcccag accaatgcca
    10381 atcaaaatgc gcagtccaat aagatgctct ggcgtggtgg caaaaaattg taaaaacgaa
    10441 gcaagcgtaa tcagcaaaaa gctgaaggtg aagatttttt gccgaccaat atggtcggag
    10501 atccacccaa gaaccaggct gccaaggaac aaaccaagga gcgccgagcc gccgatcatt
    10561 cccgccataa acggcgtcag ttgcatggcg ggcgtaagct gaataatggc gtaaccaatg
    10621 acgccgagaa cataaccgtc ggtcaggtgt gcgccgaaag tgagcgcagc aatgcggcag
    10681 tgaaaacggt taagcggtaa atcatccatt cgcaccggtg aagtgttcat ctttacccct
    10741 tcattgtccg gtaataagaa cggcttctcg cctgagaagc cgaaaaggca acgccacgtc
    10801 atcgcggaac gctgcctgac acattatttt tcaatcggat agatagtacc cgtattcata
    10861 atgccattag gatcgaactg ctttttcagc ccttccagca acgcccacgc gctgccgtgt
    10921 tccagtttgc tccagtgaac gcgatgttta ccgataccgt ggtggtgcac catcgaaccg
    10981 ccgaggcgaa tggtttcttc acagatgatc ttgttgagcg gattgtggta cttgtcgatt
    11041 tcctcttccg gcttacagtc aacgacgttg taatcgtaga cgaagtacat gttggtgccg
    11101 ttctgatagc tatgagagga atgaccgccc agcatggtga tgtcgtcggc gtgcgggaac
    11161 tcagtacgaa tacggttaat aacgctttcg tagatttcgt ggatgcagct ccagcagccg
    11221 gacacttcag tggtaaagcc catgttgccg gttttgagga tctgcacacg ttcggcagcc
    11281 actttatccg gtccccagtt caggttgtta aaccaggttt cgatcagttt gctgtccacg
    11341 cgctggcact gcgggtagcg ggcaacgatt tccgcaatcc cttcgcccgt cgccttcgca
    11401 atgcgaggat tgccttcagc cataaagatc agcacacatt taccgtcggc aaaatgggtg
    11461 aagtgttggg tgccatcttc agcgtcatac aaacgagcga tcgacggacg atacccttcc
    11521 accatgattt cacgcaggat gttgaagccg gttttcatgt cttccaggat atagccgtag
    11581 aagaggttgt tttccggggt gaatttaaag attttcactg ttacttcagt gatatagcac
    11641 aatgcacctt cgttgccgat gatgatgtga cgaatgtccg ggccagccgc gcggcgtggc
    11701 acgtttttaa tgcgtgtgac ggtgccatca gccaatactg cttccagacc aacgaccata
    11761 tcttcgattg cgccgtagag tgtggagaac tgcccgatac tgcgggttgc taccaggccg
    11821 cccatctgag ccagcggctt tgactgcgga gaatgccccg tggtgtaacc tttttcacgc
    11881 aacgcatttt ccagcacctc cagcggaacg ccacattgcg ccgtcgcctg catattctca
    11941 atatcaatat taatgatttg attcatggcg gagccgtcga gcaccaccga gttttccaca
    12001 acggtttcca gcccaccttc agtggcggaa gcgccggtac gcggcacacc gttaattttg
    12061 tgcgcattca taaaattcag cacacgggag acttgttctg tggaaccgag ttttacgacc
    12121 gctgccggaa tcggcaaagt ataaatacca tgaatatccg gaaatttacg aaaacggtca
    12181 atactgttct tctttaatac tgtttcatcg gtaattacgc gatctgcacc aacaatttcc
    12241 tttagctggt cgacaatcgc tgcgcgagat aaagacatag taattccttc ctgataataa
    12301 aaatggaata atcattaata agagaaaaca gaaaccaccg ttatggatta gcgcactaaa
    12361 taaccaccat cgaccactaa taaatgccca ttgacataat ttgaagccgg acttgcgagg
    12421 aatacagctg cgcccattaa atcctgagta tcgccccaac ggtttgccgg aatatgatca
    12481 agaacgcgct gattggtttc tggattactg cgtgtcgcca gcgtaatatc tgttgcataa
    12541 tagccagggg cgataccatt tacctgaata ttatattgac ctagctcatc acaataagct
    12601 ttggtgaacc cggcaagagc atgtttagtg gcagaatatg caggtgacca ttgtccacct
    12661 aagtaagaga acaatgaaca gatattaata attttgccgc ttttctgcgg gatcataatt
    12721 tttgcagctt catagcttaa ctcgaatgcg gcggtcaggt tcacatcaat catcggatcc
    12781 cagtcggcac gaccgaagtc cagcacctta ttcagcttac aaataccggc attgtttacc
    12841 agaatatcaa ctgtaccgaa acgctcacag caagcagcga taatcttctg cggcgcgcct
    12901 tctgcggtga tatccacctg catgaagtcc acctcaacac cctgtttttc aatcatttcc
    12961 tttgtttcgc cgttatcttt gacgaaacta ggaataaaga tatttgcgcc agctttggcc
    13021 aacgccatgg caaatgcctg gcctaaaccg ctattcccac cggtaacaat tgcggtttta
    13081 cctttcagtg agaaaaaatc cattgagaac gcattgagag attcgattga catagttagc
    13141 tccaatttcc gtaaggcaaa aaaaaagaaa ggtaacttcc cccgcgagag ggaagttacc
    13201 tttctcatta tctctggtaa ctggagataa tttttagcat aacggcataa ctgctaatgt
    13261 gatcgtaatc acagtgtgat attcgtattg tcaaatagtt ttaaaaagca gaagcgtgat
    13321 tactctcaca caacacctct gctttccatg ttatttctta ttgcaattac tagagacctg
    13381 agaaaagtaa tccacctgaa agggcggctt acttttctct ttttttgtct attacaaatg
    13441 ggaatacgat atgcaacaca actcatatcg ccgttggata accctcgcga taattagttt
    13501 tagcggcggc gttagtttcg accaagctta tttacgttat atttatcaaa ttcccatggc
    13561 gaaatttatg ggattcagca ataccgagat aggtttaata atgagtacct ttggtattgc
    13621 ggctattatt ctttatgccc ccagcggcgt tattgccgat aaattttcac accgcaaaat
    13681 gattacttcc gcgatgatca ttaccggatt actgggtctg ttaatggcaa cgtatccacc
    13741 gctgtgggta atgctctgta ttcaggtcgc ctttgcgata acgacgattt taatgctgtg
    13801 gtcggtgtcg attaaagctg cctcgttgct gggcgatcat agtgagcaag ggaaaattat
    13861 gggctggatg gaagggctgc gcggcgtcgg tgtaatgtcg ctggcggtgt ttaccatgtg
    13921 ggtcttttct cgctttgcac cggatgacag cgccagcctg aaaacagtca ttatcatcta
    13981 cagtgtggtt tacatcttgt tggggattct gtgctggttt tttgttagcg ataacaacaa
    14041 cctgcgcagt gccaataacg aagaaaaaca gtcattccag cttagcgaca tccttgccgt
    14101 tttgcgtatc agcaccacct ggtattgcag catggtgatt tttggtgtct tcacaatcta
    14161 cgccattctg agttactcca ccaactatct gaccgaaatg tacggcatgt cgctggtggc
    14221 agcgagctac atggggattg tgatcaacaa aatcttccgc gcgctgtgcg gcccacttgg
    14281 cggcattatc accacctaca gtaaagtgaa atcccctacc cgcgtgatcc aaatcctttc
    14341 cgtactcggc ctgctggcgt taactgccct gctcgtcacg aactctaacc cgcaatcggt
    14401 cgcgatgggg attggcctga ttttactgct gggattcacc tgttacgcct cacgcgggct
    14461 gtactgggcc tgccctggcg aagcgagaac accgtcttac attatgggca ccacggtagg
    14521 tatttgttcg gtgattggat tcctgccgga tgtcttcgtt tacccgatta tcggccactg
    14581 gcaagacacc ctgcccgcag cagaagccta ccgcaatatg tggctgatgg gaatggcggc
    14641 gcttgccatg gtgattgtct ttaccttttt gctgttccaa aaaattcgta ctgctgatag
    14701 cgcccccgca atggctagca gcaagtaagc caggaaaatt acaggagaaa aatcatgtcg
    14761 aaaaaataca tcatagggat tgatggcgga agtcagagca caaaagtggt gatgtacgat
    14821 ctggaaggta acgtggtttg cgaaggcaaa ggcttattac agccgatgca cacgccagat
    14881 gccgatactg cagaacatcc tgacgacgat ttatgggcat cattatgttt tgccggtcac
    14941 gatttgatga gtcagtttgc cgggaataaa gaagatattg tcggtattgg tctgggatcc
    15001 atccgttgct gtcgtgcgtt attgaaagcc gatggcacgc ctgctgcgcc gttgattagc
    15061 tggcaggatg cacgcgttac acgcccttac gaacacacta atcctgacgt ggcatatgtc
    15121 acctcttttt cgggttatct gacgcatcgc ttaaccggcg agtttaaaga caatatcgcc
    15181 aactattttg gtcagtggcc ggtggattat aagagctgga catggagcga agatgctgcg
    15241 gtaatggata agtttaatat cccccgtcat atgctgtttg atgtgcaaat gcctggcacc
    15301 gtcctcggac atatcacacc acaagccgca ctggcgacac atttcccggc aggactgccg
    15361 gttgtttgta ccaccagtga taaaccggta gaagctctgg gggccggatt actggatgat
    15421 gaaactgcgg taatttctct tggcacttac atcgcattga tgatgaacgg caaagcactg
    15481 ccgaaagatc cggtggcgta ctggccgatt atgtcttcta ttccgcaaac attgctgtat
    15541 gaaggttacg gtattcgcaa aggtatgtgg acggtgagct ggctgcgtga catgttaggc
    15601 gagtcgttaa ttcaggatgc cagggcgcag gatctttcac cggaagattt actcaacaag
    15661 aaagcctcta gtgtgccacc tggctgtaat gggctgatga cggtgctgga ctggctgacc
    15721 aatccgtggg aaccgtacaa acgcgggatt atgatcggct ttgattccag catggattac
    15781 gcatggatat atcgttcgat attggaaagc gtggcgctaa cgctgaagaa caattacgac
    15841 aatatgtgta atgaaatgaa tcactttgcg aagcatgtga tcattactgg cggcggttcg
    15901 aacagcgatc tgtttatgca aatttttgcc gacgtgttca accttccggc acgccgtaac
    15961 gccattaacg gttgtgcaag tctgggagca gcgattaata cagcggtagg tctggggcta
    16021 tacccggatt acgcaacggc tgttgataag atggttcgcg tgaaagatat ctttataccg
    16081 attgagagca atgccaaacg ctacgacgcg atgaataaag gcattttcaa agacctaacc
    16141 aaacatactg atgtgatcct gaaaaaatcg tatgaagtga tgcatgggga attggggaat
    16201 gtggattcga tccagagctg gtcgaatgcg taagtgagga aggccgggcg ggaaactgcc
    16261 cggcctgaac atacctgaat ggttatcccc gctgacgcgg ggaacataag ttcgacaact
    16321 taaaaaatga taacaagtcc ggtttatccc cgctgatgca gggaacataa taaaaacttt
    16381 aactagttcg gtttatccct gtttttacaa ggaatattaa ctacactcga tgtctttaga
    16441 ataaaagaca atacgaatcg cactttatca attttcactt aaagtatgaa ctggatatct
    16501 ttattcataa gaactattca tcaacagcgt atagaggcgg ttatgtaaaa ccactcatta
    16561 gcctcaaaac aaagacaaac ttccagattt attacttaac caatgaataa caagcttaaa
    16621 aaacata
//
LOCUS       DAZGSP010000113        16467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZGSP010000113 DAZGSP010000000
VERSION     DAZGSP010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.114"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(6..347)
                     /locus_tag="ACHK74_004108"
     CDS             complement(6..347)
                     /locus_tag="ACHK74_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0222833.1"
                     /translation="MDEIKTLHWCPREGMQVTEKPSVVTVKFGDGYQQRRPAGLNAQL
                     KTFQVVFRVTTDAEREALSAFLSWHGGYRAFLWKPPKHNRTVRVVCREWSITDNARYS
                     DFSCTIEQVVG"
     gene            complement(340..3582)
                     /locus_tag="ACHK74_004109"
     CDS             complement(340..3582)
                     /locus_tag="ACHK74_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0222834.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASAAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGILRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     ATKTLSGALNGVAGNIDTVATAAGVLVAVGVARYFGNLASGAMSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAVTQERLNRNIAART
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESAHQYALT
                     IDEIAHKTPSMSLPEASDNEGRTREALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGNGFMINHLTSVKTVTEGLAQATEQFAVEQSRLAQMQEKAQSIQDVLAGLEERR
                     VVLIRQQAAEQNKAYQSLLIMNGQHTEFNRLLGLGNELLQQRQGLASVPLRLPQATLD
                     DKQQGALNNTERQLALSRLKGEEKERARLGYAADDLGLVGDTYQEARQRYIRNSMEAW
                     RNNEANKPKSRAGKSGAEKAEDSFSRLLKQQKEQLALAGKNTELAKLKYQTSQGELKT
                     LTEIQKQELLRNAALIDQKKIREQLRAREETLKNDNADARASNDAELLGYGQGERVRE
                     RMRELQQIRDGYRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQEAFYAES
                     DAQRADWQKGLQEGLSNWVDSASDYASQAAQLATDGISGMVNNITEMLNGNKVEWRSW
                     AASILQEISKVLMNAAIVNGIKMAANSMSGAGGFFGSIGNWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGANGQIGPEAAGTLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            complement(3630..3839)
                     /locus_tag="ACHK74_004110"
     CDS             complement(3630..3839)
                     /locus_tag="ACHK74_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFP0222835.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEHALSFAAQS"
     gene            complement(3935..4309)
                     /locus_tag="ACHK74_004111"
     CDS             complement(3935..4309)
                     /locus_tag="ACHK74_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFP0222836.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QDVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESVRKK"
     gene            complement(4324..5040)
                     /locus_tag="ACHK74_004112"
     CDS             complement(4324..5040)
                     /locus_tag="ACHK74_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFP0222837.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGVLLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(5106..5450)
                     /locus_tag="ACHK74_004113"
     CDS             complement(5106..5450)
                     /locus_tag="ACHK74_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFP0222838.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVAVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(5447..5893)
                     /locus_tag="ACHK74_004114"
     CDS             complement(5447..5893)
                     /locus_tag="ACHK74_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFP0222839.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(5890..6240)
                     /locus_tag="ACHK74_004115"
     CDS             complement(5890..6240)
                     /locus_tag="ACHK74_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP0222840.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDET
                     GGRLEILCRLGGEK"
     gene            complement(6250..6576)
                     /locus_tag="ACHK74_004116"
     CDS             complement(6250..6576)
                     /locus_tag="ACHK74_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP0222841.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMEKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(7158..9319)
                     /locus_tag="ACHK74_004117"
                     /pseudo
     CDS             complement(7158..9319)
                     /locus_tag="ACHK74_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(9265..9486)
                     /locus_tag="ACHK74_004118"
     CDS             complement(9265..9486)
                     /locus_tag="ACHK74_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222842.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(9531..11468)
                     /locus_tag="ACHK74_004119"
     CDS             complement(9531..11468)
                     /locus_tag="ACHK74_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP0222843.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLAKPVPDMPSQLAARLDEAWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRQFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASSGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            complement(11532..13193)
                     /locus_tag="ACHK74_004120"
     CDS             complement(11532..13193)
                     /locus_tag="ACHK74_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP0222844.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDEPWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVERGEHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWTGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHEIAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLEHIGIDPSGVGQIL
                     DSLVEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            complement(13190..13753)
                     /locus_tag="ACHK74_004121"
     CDS             complement(13190..13753)
                     /locus_tag="ACHK74_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP0222845.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            complement(14043..14408)
                     /locus_tag="ACHK74_004122"
     CDS             complement(14043..14408)
                     /locus_tag="ACHK74_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP0222846.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            14450..14650
                     /locus_tag="ACHK74_004123"
     CDS             14450..14650
                     /locus_tag="ACHK74_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0222847.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            complement(14782..15108)
                     /locus_tag="ACHK74_004124"
     CDS             complement(14782..15108)
                     /locus_tag="ACHK74_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFP0222848.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
     gene            complement(15509..15694)
                     /locus_tag="ACHK74_004125"
     CDS             complement(15509..15694)
                     /locus_tag="ACHK74_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP0222849.1"
                     /translation="MRELKMKLCVLILPLVVSACGSTPPAPVPSVKPPAPPAWIMQPA
                     PDWQTPLNGIILSSENG"
     gene            complement(15917..16048)
                     /locus_tag="ACHK74_004126"
     CDS             complement(15917..16048)
                     /locus_tag="ACHK74_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222850.1"
                     /translation="MSDKLIMLAKILCVIVGISFSLMLVALFLSMGWMMLSSSGMLG"
     gene            complement(16063..16245)
                     /locus_tag="ACHK74_004127"
     CDS             complement(16063..16245)
                     /locus_tag="ACHK74_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222851.1"
                     /translation="MEKYKRILLRLTMKNGLELKAPVTDDISRAQAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKA"
     gene            complement(16402..>16467)
                     /locus_tag="ACHK74_004128"
     CDS             complement(16402..>16467)
                     /locus_tag="ACHK74_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0222852.1"
                     /translation="CYGQVSRRDQESALACWGIDR"
BASE COUNT         3503 a         5309 c         3916 g         3739 t
ORIGIN      
        1 aattctcatc caactacctg ctctatcgta caactgaaat cactgtaccg ggcgttatcc
       61 gtaatactcc actcccggca caccaccctg accgtccggt tatgtttcgg gggcttccac
      121 aaaaaagccc ggtaaccacc atgccatgac agaaacgcgg acagtgcctc ccgctcagca
      181 tccgttgtca cccgaaaaac cacctgaaag gtcttcagtt gcgcattcag tcctgccgga
      241 cgacgttgct gataaccgtc gccaaacttc accgtcacca ccgacggttt ctccgtcacc
      301 tgcatccctt cccggggaca ccagtgaagg gtcttaatct catccactca gcattcctcc
      361 gtcacgacgc atggataaca tcaccgcctg tacccgctgg tcaatcagtt gcacaagggt
      421 gcccgccgct tccggcccta tctgcccgtt agcgccgtca ttctgaatgg caatgtggta
      481 caccggggaa tacaccagac ccgcactgcc gttcatactg cccaccgcac gcacgccgag
      541 tgaaccatcc gctgcccggg tcaggggcat gatggcttca ggtccggctt cccccatcag
      601 ccctgcccct tttgcaaagg caaagtacgt gggcgtgtcc acaatactgt tactgtatgc
      661 actcaggttt gccgaggtat aaacgccgcc ttttgcattt gccaccgcgc cacccagcca
      721 gttgcctata ctgccgaaaa atcctcctgc accggacata ctgtttgccg ccatcttaat
      781 gccgttgaca atggccgcat tcataagaac ttttgatatt tcctgcagga ttgatgcagc
      841 ccagctgcgc cattccactt tatttccgtt cagcatctcc gtgatgttat tcaccatccc
      901 tgagataccg tctgtcgcaa gctgtgctgc ctgtgaagcg taatctgatg cgctgtccac
      961 ccagttactt agcccttcct gcagcccttt ctgccagtca gcacgctgcg catccgattc
     1021 ggcataaaaa gcctcctggt ctttaaggcg ttcactcaga tactgcgcat tctgcgccag
     1081 cgcctgtctg taaaaatcct cactgatatc ccctgtctga tactgagact gcaggtccgc
     1141 atccttctgg cggtagccgt cacgaatctg ctgcagctcc cgcatccgtt cacggacccg
     1201 ctcaccctgc ccgtacccca gcagctcagc gtcatttgat gcccttgcgt ctgcattatc
     1261 atttttcagg gtctcctccc gggcccgtaa ctgctcccgg attttcttct ggtcaatcag
     1321 ggctgcattg cgcagcagct cctgcttctg tatctccgtc agggttttca gctcgccctg
     1381 cgaggtctgg tatttcagtt ttgccagttc tgtattcttc ccggccagtg ccagttgttc
     1441 cttctgctgc ttcagcagac gggaaaaact gtcttccgct ttttccgccc ctgattttcc
     1501 ggcccgggat ttgggtttat tcgcctcatt attgcgccag gcttccatcg aattacggat
     1561 gtaacgctgc ctcgcctcct gatacgtatc ccccaccaga ccaaggtcat ccgccgcata
     1621 ccccagccgg gcacgctctt tttcttcccc tttcagccgg gacagggcaa gttgacgctc
     1681 tgtgttattc agggcgccct gctgtttatc atccagagtg gcctgtggca gtcgcagcgg
     1741 tacactcgcc agtccctgac gctgttgcag cagttcatta cccagcccca gcaggcggtt
     1801 gaattccgta tgctgaccgt tcatgatcag cagtgactga tacgctttat tctgctctgc
     1861 cgcctgctga cgaattaaca caacacggcg ctcttccagt ccggcaagca catcctgaat
     1921 ggactgcgct ttttcctgca tctgtgccag acgggactgc tcaacagcaa actgctctgt
     1981 tgcctgagca agcccttccg ttacagtttt caccgatgtc agatggttta tcatgaagcc
     2041 gttaccggtc gtccagcccg gattagccag cacatactga tacccggcga ttttttcctg
     2101 cagggatttc acccggctgg cctgctcatc aatcagccgg ttctgctctg tcagcgcctc
     2161 ccgtgttcgc ccttcattat ctgaggcttc aggcagagac attgatggcg ttttatgcgc
     2221 gatttcatca atcgtcagtg catactggtg cgcagactcc ctggcctgtt cctgattctg
     2281 gtacagcgta taccatgcgg cagctcccag catcaccagt ccgggtacgc ctccaaccag
     2341 tcccagcgca ccgctcatca gacgtgagcc caccgccgtt gtactgttca gcgcattctg
     2401 ggcggcggtt ctggcagcaa tatttctgtt caggcgttcc tgtgtgaccg ccagacgggc
     2461 ctctgccgca atctgcatct cagtcccgcg ggctgccgcc acagcctgct gagcccggta
     2521 cacggctgcc cttgcccgcg ccgtggcaat ctgcgttccc cttaactggg cctcagccag
     2581 tgccacttca ttacgtgcag cggtcacaag tcctgccgtg gcagacatcg ctccggaagc
     2641 cagattacca aagtaccggg ccaccccgac agcaaccagc acacccgcag ctgttgccac
     2701 ggtatcaata tttccggcca caccgttcag tgcgccggag agcgttttcg tcgctccgct
     2761 ggcctcattc gcgccgccca cccaggccat aaaggcgttt tccaccttcg tgatcccgtc
     2821 agacaccgtt tccggcatgg ctgcatattc atcacgcaat atccccagct ggctgattaa
     2881 cgcgggaacg actttatccg ccgtcagttg accatcgtcc gccatcgcct tcagatcctt
     2941 acgggccacg cccatgcctg cagccagtgc gcgaatgatc cggtccccac tttcattgac
     3001 cgaattaaat tcctcgccgc gcaacacccc ctgtgccagc gcctggctga actgggtgat
     3061 caccgaaccc gcctctgccg cactggcacc ggaaattttc agccctgtcg aaatggcctc
     3121 cgtcaccttc agcacatcat cagcactgta accatattca cgcatcgagg cagccgaacg
     3181 ggcaaacagg gccgcattat ccgaaaatgc ggtgcctgtc cgctggctga tgtccatcag
     3241 cactttctgt gatgacgaaa attcatccga tgactgcgac gcctgtttca gacgggcatt
     3301 cacggagctc cattcatccg ccagcgaaat caggtgtccg gtggcaaagg caccggcaaa
     3361 cgcaccggtc attccgacag ccgaagcgcg gatttccgtc aactggctgt tcagctcagc
     3421 cagagcccgg cgctgctccc tggctgccgc agcagcctga cgtccgccat tctgcagggt
     3481 ccggtaatat tcactgccca tacgggacgc ccgctggatc tccgactgga atgactgtga
     3541 atttgccgaa attttgataa tcagttcacg taacgtcgcc attcaccttt ctccgggcgt
     3601 aaaaaaaccg cctcagcggt tctcatcatt catgactgtg ctgcaaagct cagcgcgtgt
     3661 tccagcgccg caaacggatc cacctccggc ttatcctcat cctcgcccca gcagagcatg
     3721 gcgtccttca gtgcaacatt catcccctgt gccccgaaaa ccgctttcac gatctgtgca
     3781 ttacggatat ccccgcgctc atcacccagc ggggatatcc tgtcgaactc catccacatc
     3841 atcgcctcgc tcgcactcag gctgtgccgc agttcggata aggtgcgccc cagacggagc
     3901 gcaagtcgca tcagaaagcg aatttccggg cgggctactt ttttctgacc gactctgcat
     3961 cagcgatcag ttccagtgcc tgacgcagca agcgggcatg taccggacca tagacggcca
     4021 gcacctgctc acggtcgtcc ggagtgaaca cccgttgcag atccgtatca cacaggacat
     4081 cgcagaacag cgtcacatcc gcttccaggt tacggcgggt tttcgccacc accgacaggg
     4141 tatcatcatc ctctccatca ccattgagca cgtcctgcca cagataccag gcctctgccg
     4201 aaggctcccg cagcaccacg ctgacattac cccattccgg caccttcacc gttttatgac
     4261 gaaaccctga cagtctggcc agcgccagcg ttttcagatc ctttttcatg atgacccatc
     4321 cccttatccg gcggctgcgc tcactgtcac ggtgcattca acagacgtca cactctgtgc
     4381 tttctctgcc gaatcagtca ccacgcaggt atatttcccc gcatcggcgg actgcgcacc
     4441 tggcttactg aaggtgtctg tcgtctgccc gtcaaccggc tgaccatcct tcttccaggc
     4501 gtatttatac ggcggcgttc ccccgttggc actgactgac attgtcagca gcacaccggt
     4561 attcacggta agtgtcttat ccagattttt cacaaacgcc agcggtacca caaaggacac
     4621 cggtttgcct ttcagacgca gtgagaacgt tgcagccacc acgccgttgg taccggatga
     4681 ccaggtgtgc tgacgcactt ccgccaggaa tttaaagccc ttaccggacg gaaactgcac
     4741 cttaaacgca tacaacgcgt cattgtcata ggcatcacgc agggcgttct gggcctgatt
     4801 cagatagaaa ttacccgaca tggaaatctc ggacgacgcc cccagaccgt tgatgttctc
     4861 ctgctctgtg gagcagagcg tggtcacatc aatatcctgt ttctgaccgg cggtgaactg
     4921 gacttccttg atggtgcagt ccaggcgcag atattccgcc ttatccatag tttcagcagt
     4981 cgccggggca gatgaaatca tcacctgcgt cagctgtgag cgttcataca aagcagacat
     5041 tctgcctcct gataataaaa aatccgcacg cggcggggta tgggttttgt agaaaaaaga
     5101 aaaagtcaca ccgtgacctg aaactctagg gttgcacggt aacagcggtt ttccggaata
     5161 tagtcctgca tttcactgac ggatcccggg gccagcagca ttatggcttc acgggcgtcc
     5221 tgacgtatct gacgcgcctg cgtcacagtc ccggcataaa cgtctatctg caccgccact
     5281 gaggactccg cctgcccgcc catcacgtcc gcagacaccg atgaaatcag gctgaaaacc
     5341 acccacggaa gcgccaccga cggcctgcca tccagcaggg ggaccacata cgggtacacc
     5401 tgcccgccgg caagatgcgc cagatgagga tacaaatccg cctccgtcat cgtctcagta
     5461 cctcatcaat ggcccggttc atccgcgcaa tcgccacctg cgctgcctgt tcactgcgca
     5521 catcaaacgc cgggcgcaca aacgggtgcg gtggcatatt cacggtcccc atttccacaa
     5581 accgccagta gaaagcattg cgcgggttat ccgccttcat ggtgttatcg ctgttaccgg
     5641 tgtccggatt aacaccacgg atatggacac cggattccat cccgccatcg cgggagcgcc
     5701 gggaaaggac caccacattg cggcgcagtt ttcccctgcg caccggtgcc cgtgacacca
     5761 cttcttcttt cagcacattc gcacccgcac gggttgcctc acgcagcacc cggttatttt
     5821 ccgcaccact cagaagctgc aaatcgcggc tgatgtcctc cagccccgaa aaatccagca
     5881 gggtttcgat catttttccc ctcccagccg acagagaatt tccagacgtc cgccggtctc
     5941 atccggcacg ggcagcccga caacgttcag gatccggtca cgccatggac cactcagcac
     6001 atgaagtcgt gacgctgccg tgatttcccg accggactga ccgcgcaccc agatgcggat
     6061 ttccgcctgc gccatttccg caccggactg catccgctcc cggctgctcc tgccacggat
     6121 atccgcatga attttcccgc atgacaccca ttcttccgtc atttctccgg cagcattacg
     6181 ggttaacacc gggttcagaa cacttatcat ctgtgtcaga cgacctgcag atattgccat
     6241 tccccctcct cataacaccg tcggacaacg caaatcgtaa atcagcacgg aaacagaaaa
     6301 cggcagctcc ccctgaagca gttcttcccg ctccgcaaga tccggattcc ggtacagcat
     6361 cccggtcagt cgcatggcag cccccttcat ccgggttaat gcctcgcccg ggatcagttc
     6421 accgtcctca cgaatcactt tatcccggct gccctgaatg taggccagca gcacggcggt
     6481 agcctgacga accttctcca tcagcatgtc atcatccgcg tcatggtcga cacgcagatg
     6541 tgccttgatc tcttccagcg tcagtaatgc cgtcattttc cgcctcctgc atcccgccca
     6601 cgttttgcag ccagggccca gcctgatgaa tgagcttctc cgggtttatc accggtcata
     6661 ctgttgcagt gccacagcga gcccccccac gtcaccgtat cgccggggtg gtaggtttca
     6721 ccggctctga acacaccgcg gtagagcatc accggcaggg aaaatgtttt ttccgtacgc
     6781 tggccactgc tctgccggac caccacagag aacaaccgtt cacccgtcat gctgacgtca
     6841 atatccgcca ccccgtcaac caggcattcc catccccgca tcccgtgcgt tttttcatac
     6901 gcccgccaga gtccgccctg gtgtgtggca tacgtgcccc ggggaaagga tttttgatcg
     6961 tcaatggcag ggagtatttc cagtgcagtg gcatcacgcc cgtcctgcgg agccggcagg
     7021 gcactcaccg catcattcac cgccttctgc agaacatccg gatcatagtc acgaccatca
     7081 cgcggaacag gaatatggct taccgcctcc ttcaccatct gctcaagcat cggacgcaca
     7141 tcatccgggg tgatactttt accgtctgcc ggctgcggaa tatttgcgac tgcatcattc
     7201 accgccttct gcagtacttc cggatcgtag tcacgaccat cacgcggaac aggaatatgg
     7261 ctcactgcct ccttcaccat ctgctcaagc atcggacaca catcatccgg ggtgagactt
     7321 ttaccgtccg ccggctgcgg aatatttgcg actgcatcat tcaccgcctt ctgcagtact
     7381 tccggatcgt agtcacgacc atcacgcgga accggaatat ggctcactgc ctctttcacc
     7441 atctgttcaa gcatcggacg cacatcatcc ggggtgagac ttttaccgtc cgccggctgc
     7501 ggaatatttg cgaccgcatc attcaccgcc ttctgcagaa catcgggatc atagtcacga
     7561 ccgtcgcgcg gaacaggaat atggcttaca gcctccttca ccatctgctc aatcatcgga
     7621 cgcacactat cgggagtaat acttttaccg ttcgccggta ccggtatttt cccgacagca
     7681 tcattcaccg ccttctgcag aacatcggga tcatagtcac gaccgtcgcg cggaacaggg
     7741 atatggctta ccgcctcctt caccatctgc tcaagcatcg gacgcacatc atcgcccgtc
     7801 acgcacttct gtaataccac agacagggaa gccagttttt cttcaaacgt ttgtgcctgc
     7861 gcggctattt tcccctcaaa tgtgcgctgt aaatccgcca gcactgcgga aaattcttca
     7921 cccagcgcac ggataatgga cagttcacgt tcattcattt tttcagaatc cccctgaaca
     7981 tcgccttcac cgcatcatgc tctgtttcag tgattgcctt attaccgtca gaggcaacga
     8041 ccgcctgagg cacggaagct gttttaccgg acgacgcgaa cggatcttca cgggcatcac
     8101 ggcgggacaa cgcttccaga ctgtagttct gctgctgaag atacagtgca tcaccgccgg
     8161 ccaggggcgg caggttctcc cgtttacggg cctcattggg cgtgagaagc gtatttttca
     8221 ccgcatcccc cagcgttttc atgcgccgct cactgtccat tctcagcagc gtggtgacat
     8281 caaattccgt actctcgttt tcccccgttt ccagcgcctc atccagtaac agttcaatgg
     8341 actcaatcag cgtctgcagg cactgagaat aatactgctg ctccagcgcc tccacgttgt
     8401 cactggaagg cggttgtccc acgccaatct tgtaggccgg gacacggaac accgaacaga
     8461 caatttcagc ggtcatcttc agttgttcca ccgtctgcga atccaccggt gaaaacgtcg
     8521 tggggttgta ttttgccccg ttgctcagaa tggccgtttt ccccgcattt tcgcctgtat
     8581 acccgctgtc ccagttgctc ttcagttttt tcgcattttc ttccgtaata ctgccgggga
     8641 tctcaatcac cccggacggc ctgccgccat ttctgaaaaa agacgtcgaa ttttcctgaa
     8701 tatgatgccc ctgcgtggcc gccagcccgg cggcatacac cggcggcaac cctataagcg
     8761 gatgaaaaaa acagttaaac cggtcgtgga tcacttcccg ggcaggcacc gtcaccgcct
     8821 cagtgatccc gcagttccgg tccggcgtga tgcggtagaa cacctcgccg tcatccgcca
     8881 ccagaggttc aacccggttc cagtccagaa tacgcagttc tttgatctgc ccccgggcat
     8941 tacggatttt cagcaccacc gtattgccgt gacgcagttt ggcgttcagc cacagttcaa
     9001 aaaactggat gcggttctgc tgggcgttgg gacgacgaca gaggcgggca atatcccccc
     9061 ggcgcgtttc cctgcgtatc ccctgcgcat ccgtctgcat aagacgcagc cgcattttgg
     9121 cgatatcctg ggatatcagc gaaatacatg caaacaccgc atgaaaggag aggacggctt
     9181 caggatcggc tttcacgccc tgctgccagg cgccggaaaa gggctcagcc accgcctgaa
     9241 acaggctggt ccagcccgcc tctcttacgt cacgtcctga tttctggttt tttcgggttc
     9301 gccgtaaaag gttccacatt cgccatgctc cgcatcacgt ttctttttct gacctgccgg
     9361 acgtcgcacc gtgatgtact ccgcctttcc caggcgaacc agcacctccg cacacggctg
     9421 tgccacatca cggatatccc cggcccgggc atcatgcgtg ccctgcagat atcggatctt
     9481 tgccataacc tgttacggga ggcacacgcc tcccgtcctc cttatcagac tcagccgccg
     9541 gacgcactgc catagttcac tccggtgatc accgccaccg ccgcggtacg gcgacgacgc
     9601 cagttgatcc agcgctccgc acggatggcc acgctgcctg tctggaacat ggaaaccagc
     9661 tccaccggcg acggcgtggt actgtcgccg gtcggctcag actgcatttc cagtgatgcc
     9721 tcgcgggaca tatccactgc cacgccgccg tcatccgcca gataaatatc cggggcattc
     9781 accagcacca gctggtcacc cacgtactga gagacaatca ccggcagccc ctggaaggag
     9841 ccacccagca gggtcatgtc cgggtattct ttctgaccca gcgcattttt acgcatggac
     9901 agcgccagag cattggtgct ggacatcagc cagaccgcac cggtgggctg caggtttgct
     9961 gccacaaact gtccaaacgc cgcctctgca tccgcatccg ggttaccgct tgatgccgtg
    10021 cccttcacat catgggtgat ggacgccggg gagacatctg ccaccgccgc ttttttcggg
    10081 tccacaaagt ctgtatccag acgcgccacc accgcttccg ccagcgcatt acggaccagt
    10141 gcatcagcag ccggactgga aaaacggatc aattcttccg tcagtaccgc aatggccgac
    10201 accttcgcat gactgaaggt gatggattca aaatcaaact tcgtcagggg ttttgcctta
    10261 ccctccccca cccagccggc agcaccgccc gacacctggg cgtgcacacg gatattgaac
    10321 ggcacctgac gaagtgcagg gatcccgccc tgaccaaatc gcccgataat ggtctgagga
    10381 cgcaggtaat caataaagtc ctgtgcgtat tcctgatatt cagacaggct gcctgcccac
    10441 tgcggatccg tggtggtccc cgcgcccact gccgatttca ggacatgatg cagacgactg
    10501 tcatccggat actgacgacg ggccacttcc agggcttcag agcggacacc tttagccgca
    10561 gccagcgatt tggcaaaacg tgcgaaacca atccccttat ccagtttctg ctccacacgg
    10621 atcaccggcg cagaagccac cgcggccaca ttcccgttac cggcctgttt caccggctgc
    10681 gccgtggcgg ccttaccggc ttccagttca cgcaggcgct tcaggtgcgc atccacctga
    10741 cgtatttccg ctgcggtgtt gtcgtaatgc tcttcctcct ccacatccag cgttcgccct
    10801 tcctctgcgg ctttggtcat gacctcctca agggaggctg ccagtgctgc acgcttgttt
    10861 tcaaaacttt taatctgttc gccaatattc attatggtct tttccttatg aaaaacggtt
    10921 gttgactgtg ccgcagcgcc ggcagaagat gcgattttca ccaccggttt ccggttgccg
    10981 gacgcggcag aaaactggcg gtcgtaagat ttaatggtcc ggatggtgca ttccgcattc
    11041 gcgggcacgg tgacggcaga cacctccatc agctcccagc gcagaaaatg cagtccgcct
    11101 ccgtccagaa aggtgtattc atgaggacgg aagcccacgg acagccccct gaccagcccg
    11161 gtcttaatgg ccgcccaggc ctcatccagc ctggcagcca gttgcgatgg catatccggc
    11221 acgggcttcg ccagtgttgc cgtgatttcc agcccttcgc tgacccggcg caccgtacac
    11281 tgccccaccg ggcgggaatg gtcatgctgc cagagaaacg ggatcgcact gccaaactcc
    11341 gcgccctccg gctccaggat gtcaccatcc cgatccggag aaggcgttga cgcaatcccg
    11401 gtgatcaccc gctcatcctc actgaaggat ttcaccgtca gcagggagca ggcccgttta
    11461 agagtcacat cagcctcctg aaaataaaaa aaccgccgca gcggtccatg atggttacag
    11521 ggtgaacagg gttatatgaa aaaaaccgca tattctttct ttttcggctc cggattcagg
    11581 gacatcaggg ataccgcatt gaagagcgcc atcagcgggt caattttccc ccgtccgctg
    11641 gcctgtttgg taataaggat ggcgttacct ttcggctcca cccgggcatt gccaacgcac
    11701 caggccatca gtggctgacc accatgcacc agtactccct cagccagttt gcgctcggtg
    11761 gtttttatgg ccccgcccag cttccagccc tggcttatcc ccacaacaat tccgtcgggg
    11821 atcccggctt ccaccagtga atccagaatc tgccccacac ctgacgggtc aataccgata
    11881 tgctccagta actcagcctc atgaatacga cgcacatact ccgccacttc cgccgtgtca
    11941 tccccgaccc gacggacaat cgtcatgtct ccacaggcca caaaatcctg aaaccgggat
    12001 gcctcactct tccgtctgac caccgcgatt tcatgcgccc aggcatggcc ccagcccagc
    12061 cattcgcggg tttccctgtc acggccaatc acgtacattc ccagcagatc atccaggccc
    12121 ccgccgtcaa tccccaccgt caccacatca gcgcgctgca ggatatcgtc caggctgacg
    12181 cgcctgccct gctgctccca gaaatccgca cccgtccagc ggtcagaacg cagggcaaga
    12241 ccaatttcca cattggcatg ttttgacatg aagccacgaa atgcttcctc accagcctcc
    12301 cgggctttac ggtactcccg gtacagaaaa gcctcatcca ccgaataacc gagattcgga
    12361 ttgaccatgg cgaggttttc catcagcagg tgctcaccgc gctccaccat ctccggcgga
    12421 tgctcaaata tcaccggcag aaagtgcgga tcatgaattt tgccgtcacg gacatcccgg
    12481 gcgtactgca gtttctgtct gaacaccccg gctggcggct cattcgactg ggtggtcgta
    12541 tacaccacaa acccttccgg gcgggaggca aggccgccta tggcttcacg taacatgtcc
    12601 tccgctttgt actgcttgcc aaataaccac ggttcatcaa tcagcgtccc cacggacttg
    12661 ataccggata ccgtattcgg atcggcagcc accaccttaa gggtggtgtc cgtcaccctg
    12721 tgggtgatgg tccggatatg tgtctgcacc tggcagaggt catccagatc atcgtcccgt
    12781 cgtaccatat cccgcgcagg attaaaggcg ttagccgcca cctccacggt cggggccaga
    12841 atggtgtagc ccgccgcctg ccgccagttc agtaacagcg ccgtcatcat gatcccggca
    12901 gccagcgtgg acttgctgtt tttcttgggg ataaggataa aaacttcctt gatatggcgt
    12961 acaccggtct gcgcatcgta ggagccaaac agggccgcca ccaggtcaaa cacccactgt
    13021 gcgcaggact ccccgaacgt cgggctgccc ggtgcatcca caatccgcag ttgtttaaaa
    13081 atcgccagtg catgtgcagc ctgatccgga taaatcgggg ccggaataat cgacagcccc
    13141 tttttcaggc gctctgccca gtccgggcag gccgtgctcc acacaggtat catccgttgc
    13201 cctcattatc attattcacc accagtcggg gtggtggtgg caccgcaaaa cggttagccg
    13261 cttttttcgc cgcgtcacct tttgccgatt ttttaccggc atcccctttt ttgtggtgcg
    13321 tgaactgcgc cagcttataa gccgcatcca gcgccagcct ggggtcggta ttaatgttct
    13381 ccaccagaag acgccccatc gctttcaccg gatcgggaag accgtcctcc atatactcaa
    13441 taccaggaga tatcaccacg ggcggtggca tctccggatt tgtttcgtcc ggctgtggta
    13501 ttgcagccgc ctcacggcga cggggtttat cctcctgctc tgattttttc tgccggtaaa
    13561 caggaacctc atccacctcc accgtctcgc actgtttacg ggctataaac gcaagcacct
    13621 ccggatcttt tgccagctgc gagcctttaa ccctggctgt cttcgccgaa taaccggcgg
    13681 caatggctga cgctgttttg tttttcccgg acatgagcgc cagcgcaaat tttcgttttt
    13741 gcgttgtcag cacagcctcc tcccgggtcc agaacgcact cagccgggta tggttcagcc
    13801 catttttccc ggcgtctcat gccgcaaatg ttaactgctg cctggttaac atttgctgaa
    13861 aaagcctgtt aacatttttt tccacgcaac aaactgaata ataaagataa aaaccgcaaa
    13921 aatgcccgga cagccagtta acatgttaac tgccctgaaa cgggaatttt ttctctgcgt
    13981 gagagggggc gcggtgtccg gagcgatcgt tttttacgcc gaatgatacc cccccggtcg
    14041 ggttacagtc cgatgatgtc gtccgctctg ccactacctc cggacacctc cggcagcgtc
    14101 gggtccggca taccacccgc cgcttcacga gcagactttt gtcgatggca ttcggtacag
    14161 agcgtccaga gattcgtctc ctcattacca ccaccgaact gaagtgcaat tcggtgatcg
    14221 agttcactgt cacagaggtc aaccacacga ccacagagac agcactgccc ggcatccctg
    14281 agccagatat gacgcttgag ggaaacacgt gcactgccac tgacacgacg ctgttcaccc
    14341 ttcagaatat tcacccgtcg ggtattcagt gttttgattc tgctctggag tgtacgaagc
    14401 tcagccatgt aaaatccccg tcatatggca atcagtaaag gaaataaata tgtcatcgaa
    14461 aaaccggacc cgcagaacca caacccgcaa tatccgtttc cccaatcaca tgattgaaca
    14521 gatcaacatc gcccttgagc ataaagggtc cggtaacttt tcagcgtggg ttattgaagc
    14581 ctgcaggaga aggctggcaa cagatgcaac gcacctgcgc ccggccagca tgacaaataa
    14641 cgagaaatga acgttcggtt acaggagcag gtacccactg tcctccaaca atatttcatc
    14701 ttcatacccg gcggaacaag acttacccag ccgggatgta cagaataaca acagagtgat
    14761 aattaatttc tgatgaaata atcagggtgc agaaggacta aagataaacg ttttcttcac
    14821 gcctttacgc ggcctgtcct tctcaaatcg ccattttgcc atcgccttta caacctgctc
    14881 atcaaacaga tggtgcggct ctgaacggat aaactcaatt cgggtgacag taccatcagc
    14941 accaatatca aacttcacat caacccgtcc ctttatataa tttgccgctg cataggccgg
    15001 atattgtggt aatgccttaa ccaactgtcg gggcatatct gttttatgtt gcgtacagcc
    15061 cataaccaga gaagacaaca aaataattaa cggaagattt cttttcattt tcattcccgg
    15121 cgcagataag aataagtctt attctaacaa tgccaccctg tcggccatca atcctctgct
    15181 taatggcaac gacaattatc cgacttaaat cacaaatcag acacatgaca taacaggtct
    15241 tgcgaggtaa cacatcgtcc ggtttcttcc accatcgcac cggaccagcg accatgaggg
    15301 gacaacgccg cgctccgtta acgcggtaaa ccccggtgtg tatcgttttt gattatcccc
    15361 gcacactcgc gcagaggagt ctccctgtcg ggctgcggtc tctgttaatg agggaataca
    15421 gcgacgatac ggcgcatccg caaaacttag ttcaggcact gagtgcggat atagtcctgt
    15481 gccccttcca gctgcttctg cattgtcatc aaccgttctc tgaggataaa ataatcccgt
    15541 tcagcggtgt ctgccagtcg ggggccggtt gcattatcca cgccggaggt gccggtggct
    15601 tcacgctcgg taccggagca ggtggcgttg atccgcaggc gcttacgacc agcggcaata
    15661 tcagcacgca gagtttcatt ttcagctctc gcatcggcta attccctcga gtattttgca
    15721 tcgagcgcag caacatcgcg ctggcgcacc tgcatatcag taatggttgc gtttgccagc
    15781 tccagctctc tggctttttt atcacgctgc gctttgtagg tgatggcgtt atcgcggtaa
    15841 tgattcagcc ccagactaag cgcaccacag accaccagca gaataacggt aaacgcggaa
    15901 agcattcggt ttatgctcac cccagcatcc ccgacgaaga taacatcatc cagcccatgg
    15961 aaagaaaaag agcaaccagc attagtgaaa atgaaatgcc gacgattaca cagaggatct
    16021 tcgccagcat tatgagtttg tctgacatgt ttaatcctcc cttcacgctt tcaacgcaat
    16081 gaccagtttt gccagcccat acagcatcgg agacacagcg ataccgaccg ccacccactt
    16141 aatagcaaaa gcctgtgctc tgctgatgtc atcagttact ggcgctttca gttcaaggcc
    16201 gtttttcata gtcaacctca acagaattcg tttatacttt tccatgttct cccttgcctt
    16261 atccaaggtc agaaacacaa aaccccgctt gctgccaaca aacggggttt ttacttttat
    16321 tcacttaggt tttgccagtt cgcaggattt cgtgttatcc gtccgcgttg gccaacgtca
    16381 tttttcagca aaatattcgg cttatctgtc gattccccag cacgccagcg cgctctcctg
    16441 gtcacgacgg gatacctgac cataaca
//
LOCUS       DAZGSP010000114        16094 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZGSP010000114 DAZGSP010000000
VERSION     DAZGSP010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.115"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            32..652
                     /gene="entD"
                     /locus_tag="ACHK74_004129"
     CDS             32..652
                     /gene="entD"
                     /locus_tag="ACHK74_004129"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFP0222853.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(774..926)
                     /gene="hokE"
                     /locus_tag="ACHK74_004130"
     CDS             complement(774..926)
                     /gene="hokE"
                     /locus_tag="ACHK74_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFP0222854.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(1277..1429)
                     /gene="hokE"
                     /locus_tag="ACHK74_004131"
     CDS             complement(1277..1429)
                     /gene="hokE"
                     /locus_tag="ACHK74_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFP0222855.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            1398..1667
                     /locus_tag="ACHK74_004132"
     CDS             1398..1667
                     /locus_tag="ACHK74_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222856.1"
                     /translation="MTAARAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            1871..2989
                     /gene="ybdK"
                     /locus_tag="ACHK74_004133"
     CDS             1871..2989
                     /gene="ybdK"
                     /locus_tag="ACHK74_004133"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFP0222857.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFTSSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            3055..3303
                     /gene="ybdJ"
                     /locus_tag="ACHK74_004134"
     CDS             3055..3303
                     /gene="ybdJ"
                     /locus_tag="ACHK74_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFP0222858.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            3368..3736
                     /gene="ybdF"
                     /locus_tag="ACHK74_004135"
     CDS             3368..3736
                     /gene="ybdF"
                     /locus_tag="ACHK74_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP0222859.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            3830..4483
                     /gene="nfsB"
                     /locus_tag="ACHK74_004136"
     CDS             3830..4483
                     /gene="nfsB"
                     /locus_tag="ACHK74_004136"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFP0222860.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            4591..5838
                     /gene="ybdG"
                     /locus_tag="ACHK74_004137"
     CDS             4591..5838
                     /gene="ybdG"
                     /locus_tag="ACHK74_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFP0222861.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(5906..7282)
                     /gene="pheP"
                     /locus_tag="ACHK74_004138"
     CDS             complement(5906..7282)
                     /gene="pheP"
                     /locus_tag="ACHK74_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFP0222862.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(7384..10527)
                     /gene="cusA"
                     /locus_tag="ACHK74_004139"
     CDS             complement(7384..10527)
                     /gene="cusA"
                     /locus_tag="ACHK74_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFP0222863.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSTVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(10539..11762)
                     /gene="cusB"
                     /locus_tag="ACHK74_004140"
     CDS             complement(10539..11762)
                     /gene="cusB"
                     /locus_tag="ACHK74_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFP0222864.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(11778..12110)
                     /gene="cusF"
                     /locus_tag="ACHK74_004141"
     CDS             complement(11778..12110)
                     /gene="cusF"
                     /locus_tag="ACHK74_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFP0222865.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(12268..13641)
                     /gene="cusC"
                     /locus_tag="ACHK74_004142"
     CDS             complement(12268..13641)
                     /gene="cusC"
                     /locus_tag="ACHK74_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFP0222866.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGG"
     gene            13798..14481
                     /gene="cusR"
                     /locus_tag="ACHK74_004143"
     CDS             13798..14481
                     /gene="cusR"
                     /locus_tag="ACHK74_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFP0222867.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            14471..15919
                     /gene="cusS"
                     /locus_tag="ACHK74_004144"
     CDS             14471..15919
                     /gene="cusS"
                     /locus_tag="ACHK74_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFP0222868.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         3910 a         4312 c         3960 g         3912 t
ORIGIN      
        1 gcgtttatca tcagtctcgg atatggtcga tatgaaaact acgcatacct ccctcccctt
       61 tgccggacat acgctgcatt ttgttgagtt cgatccggcg aatttttgtg agcaggattt
      121 actctggctg ccgcactacg cacaattgca acacgctgga cgtaaacgta aaacagagca
      181 tttagccgga cggatcgctg ctgtttatgc cttacgggaa tatggctata aatgtgtgcc
      241 cgcaatcggc gagctacgcc aacctgtctg gcctgcggag gtatacggca gtattagcca
      301 ctgtgggact acggcattag ccgtggtatc tcgtcaaccg attggcattg atatagaaga
      361 aattttttct gtacaaaccg caagagaatt gacagacaac attattacac cagcagaaca
      421 cgagcgactc gcggactgtg gtttagcctt ttctctggcg ctgacactgg cattttccgc
      481 caaagagagc gcatttaagg catcggagag acaaactgaa gcaggttttc tggattatca
      541 gataattagc tggaataaac agcaggtcat cattcatcgt gagaatgaga tgtttgctgt
      601 gcactggcag ataaaagaaa aaatagtcat aactctgtgc caacacgatt aattgacaac
      661 atctggtacg attcgcccgc agccatcact gaccgcgggc gaaagtgtaa agcaggtgcc
      721 ttaccactct gacctgacaa ccggatatgc ggggattgct ccccgcacaa cggctacttc
      781 ttcggttcgt aagcgagaac agccttaaac tcaatattac gttcctttac ggtgaactca
      841 cacagcgagt ctccgaccag aagcgtaaat cccagtaccg tcaaacacag cactatgact
      901 gccacaaggg catatttcgt cagcatgttt atattgcctc catgcgaaga agggactacc
      961 atccgagttg ttgaggctcg gagtggcagc cccaggattg atagaaatat ctcctggggc
     1021 ttcatctttc cagacctcag agttaacctg aaaccggaaa gcttcaggca cccgccgtta
     1081 tcttaccctt cctcaacaaa atagctgttt atttatacag tgaatcctgt ttcggacttc
     1141 tgtcggcaaa actcatcatt gctcttaaaa cgaaatcgcc cgcagccatc actgaccgcg
     1201 ggcgaaagtg taaagcaggt gccttaccat cctgacctga caaccggata tgcggggatt
     1261 gctccccgca caacggctac ttcttcggtt cgtaagcgag aacagcctta aactcaatat
     1321 tacgttcctt tacggtgaac tcacacagcg aatccccgac cagaagcgta aatcccagca
     1381 ccgttaaaca cagcactatg actgccgcaa gggcatattt cgtcagcatg tttatattgc
     1441 ctccatgcga agaagggact accatccaaa ttgttcaggt ttgggtaggt agccccaggg
     1501 ttgatagaaa tatcgtcctg gggcttcatc tttccagacc tcagagtaaa cctgaaaccg
     1561 gaaagcttca ggcacccgca gttatcttac ccttgcttag caaaatagct gtttatttat
     1621 acagcaagtc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
     1681 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagccgtg ccataacttg
     1741 tcacattgca aaagtagcca aattgcttca gtcaccatca ccaaaaaaag aaacattcca
     1801 gaacttccgc atctcgccca gccgctccga cttctatact gaatagaaaa cgccaactta
     1861 agagaaacct atgccattac ccgattttca tgtttctgaa ccttttaccc tcggtattga
     1921 actggaaatg caggtggtta atccgccggg ctatgactta agccaggact cttcaatgct
     1981 gattgacgcg gttaaaaata agatcacggc cggagaggta aagcacgata tcaccgaaag
     2041 tatgctggag ctggcgacgg atgtttgccg tgatatcaac caggctgccg ggcaattttc
     2101 agcgatgcag aaagtcgtat tgcaggcagc cgcagaccat catctggaaa tttgcggcgg
     2161 tggcacacac ccgtttcaga aatggcagcg acaggaggta tgcgataacg agcgctatca
     2221 acgaacgctg gaaaactttg gttatctcat tcaacaggcg accgtttttg gtcagcatgt
     2281 ccatgttggc tgcgccagtg gcgatgacgc catttatttg ctgcacggcc tgtcacggtt
     2341 tgtgccgcac ttcatcgccc tttctgccgc gtcgccgtat atgcagggaa cggatacgcg
     2401 ttttacctcc tcacgaccga atattttttc cgcctttcct gataatggcc cgatgccgtg
     2461 ggtcagtaac tggcaacaat ttgaagccct gtttcgctgt ctgagttaca ccacgatgat
     2521 cgacagcatt aaagatctgc actgggatat tcgccccagt cctcattttg gcacggtgga
     2581 ggttcgggtg atggataccc cgttaaccct tagccacgcg gtaaatatgg cgggattaat
     2641 tcaggctacc gcccactggt tactgacaga acgcccgttc aaacatcagg agaaagatta
     2701 cctgctgtat aaattcaacc gtttccaggc ctgtcgctat gggctggaag gcgtcattac
     2761 cgatccgcac actggcgatc gtcgaccgct aacggaagac accttgcgat tgctggaaaa
     2821 aatcgcccct tccgcacata aaattggtgc atcgagcgcg attgaggccc tgcatcgcca
     2881 ggtcgtcagc ggtctgaatg aagcgcagct gatgcgcgat ttcgtcgccg atggtggctc
     2941 gctgattggg ctggtgaaaa agcattgtga gatctgggcc ggtgactaag tagggaattg
     3001 ccttacagaa gaatatccct gacaatggtg ttttaactta catttaacaa tgttatgaaa
     3061 caccctttag aaaccttgac taccgcagca ggcattttgc tgatggcttt cctctcttgc
     3121 ctgctgctgc ccgcccccgc actggggctg acgctggcac aaaaactggt gaccatgttc
     3181 catctgatgg atcttagcca gctttacact ttattgtttt gtctgtggtt tttagtgctg
     3241 ggcgctattg agtattttgt tctgcgcttt atctggcgac gctggttctc gctggcggat
     3301 taaacgtcga ttatcgccgt acttcgcggc atactttgct tattctcctt cgcctaaagg
     3361 aacgtttatg gataagcaat cactgcacga aacggcgaaa cgcctggccc ttgagttacc
     3421 ctttgtcgag ctttgctggc cttttggccc ggagttcgat gtttttaaaa ttggcggcaa
     3481 aatttttatg ctgtcgtcgg agctgcgcgg cgtccccttt atcaatctga agtccgatcc
     3541 acaaaaatcc ctgttaaatc agcagatata cccgagcatt aagccagggt atcacatgaa
     3601 taaaaagcac tggatttcgg tgtatcccgg cgaggaaatc tccgaagcgt tacttcgcga
     3661 tctgatcaac gattcgtgga atctggtggt tgatggtctg gctaaacgcg atcaaaaaag
     3721 agtgcgtcca ggctaaagcg gaaatctata gcgcattttt ctcgcttacc atttctcgtt
     3781 gaaccttgta atctgctggc acgcaaaatt actttcacat ggagtcttta tggatatcat
     3841 ttctgtcgcc ttaaagcgtc attccactaa ggcatttgat gccagtaaaa aacttacccc
     3901 ggaacaggcc gagcagatca aaactctcct gcaatacagc ccatccagca ccaactccca
     3961 gccgtggcat tttattgttg ccagcacgga agaaggtaaa gcgcgtgttg ccaaatccgc
     4021 tgccggtaat tatgtgttca acgaacgcaa aatacttgat gcctcgcacg tcgtggtgtt
     4081 ctgtgcaaaa accgcgatgg acgatgcctg gctgaagctg gttgttgacc aggaagatgc
     4141 tgatggccgc tttgccacgc cggaagcgaa agccgcgaac gataaaggtc gcaagttctt
     4201 cgctgatatg caccgtaaag atctgcatga tgatgcagag tggatggcaa aacaggttta
     4261 tctcaacgtc ggtaacttcc tgctcggcgt ggcggcgctg ggtctggacg cggtacccat
     4321 cgaaggtttt gacgccgcca tcctcgatgc agaatttggt ctgaaagaga aaggctacac
     4381 cagtctggtg gttgttccgg taggtcatca cagcgttgaa gattttaacg ctacgctgcc
     4441 gaaatcacgt ctgccgcaaa acatcacctt aaccgaagtg taattctctc ttgccgggca
     4501 tctgcccggc tatttcccct cagattctcc tgatttgcat aaccctgttt cagccgtcat
     4561 cataggctgc tgttgtataa aggagacgtt atgcaggatt taatatccca ggttgaagat
     4621 ttagcgggta ttgagatcga tcacaccacc tcgatggtga tgattttcgg tattattttt
     4681 ctgaccgccg tcgtggtgca tattattttg cattgggtgg tactgcggac cttcgaaaaa
     4741 cgtgccatcg ccagttcacg gctttggttg caaatcatta cccagaataa actcttccac
     4801 cgtttagctt ttaccctgca ggggattatc gtcaatattc aggcggtatt ctggctgcaa
     4861 aaaggcaccg aagcggcaga tattctgact acctgcgcgc agttgtggat catgatgtat
     4921 gcgctgcttt cagtcttctc gttgctggat gtgattttga atctggcgca gaaattcccg
     4981 gcggcatctc agttaccgct gaaagggata tttcagggga ttaaactgat cggcgcgatt
     5041 ctggtcggca ttttgatgat ctcgctgctg attggtcagt cgccagcgat tctgatcagc
     5101 ggtcttggtg caatggctgc cgtgctgatg ttggttttta aagatccgat tcttggtctg
     5161 gtggcaggta ttcagctttc cgcgaacgat atgctgaaac tgggcgactg gctggagatg
     5221 ccgaaatacg gcgcggatgg cgcggtgatc gatattgggt taaccaccgt caaagtgcgt
     5281 aactgggaca ataccattac cactattccc acctggtctc tggtttctga ctcctttaaa
     5341 aactggagcg ggatgtcagc atctggcggg cgacgtatta agcgcagtat cagtattgat
     5401 gtcaccagta ttcgttttct tgatgaagac gaaatgcaac gtctgaataa agcgcatttg
     5461 ttaaagcctt atttaaccag ccgccatcag gaaattaatg agtggaatcg ccagcaaggt
     5521 tctacggagt cgatattaaa cctgcgccga atgaccaata ttggaacctt tcgtgcctat
     5581 ctgaacgaat atctgcgtaa ccatccgcgg attcgtaaag atatgacttt aatggtacgc
     5641 caactggctc caggtgataa cggtttaccg ctcgagatct atgcgtttac caacaccgtg
     5701 gtgtggctgg aatatgaaag cattcaggct gatatattcg atcacatatt tgcgattgtc
     5761 gaagagtttg gtctgcgact tcatcagtcg ccaaccggca atgatattcg ctctctggcg
     5821 ggtgcattta agcagtaatt aaaaaaaccg ctctcatcga atggatgaga gcggacatcg
     5881 ttttgtcgga tgtagcgtga atgccttatt tccgacgcag cgttttaaat gccacaaaca
     5941 ggaatacaat ccacaccggc agcaggatcg ctgacaagcg catatcatcc atcgtgcaca
     6001 tcagcagcaa aatcatgccg aggaaggcaa tgcaaagata gttgccgaac ggatagagca
     6061 gcgccttaaa ctgtgtttca cgcccctgac gtcgcatcgc tgcacgaaaa cgcagatgcg
     6121 ccaggcagat cataatccag ttcaacagca gcgttgctac caccagcgcc atcagcagac
     6181 caaacgcttt ttgcggcagc agatagttga ttaataccac cagcgaagtg atcgctccgg
     6241 aaagcatcag cgagttaatc ggcacaccgc gacggctgac gcgagtcaaa aacttcggcg
     6301 cattaccctg cacagaaagg ccaaacagca tgcggctgtt agagtaaacc ccgctgttat
     6361 acactgacag cgatgctacc agaatgacga agttcagcgc agaagctacc acgttgctgt
     6421 cgagattatg gaaaatcatc acaaacgggc tactgttgga tttcacttcc acccacggat
     6481 agagcgccag taaaaccacc agtgaaccga tgtaaaacag caggatgcga tacaccacct
     6541 gatttaccgc ttttggaatg cttttttccg gatcgcgcgc ttcagcggca gtaatcccaa
     6601 tcagctccag accgccgaag gagaacataa ttaccgccag cgacaaaatc agcccattcc
     6661 agccggtggc aaagaaacca ccgtagcgcc agaggttgtc gatactggct ttctcgccgc
     6721 cgtgaccaga aaacagcagc cacaggccaa agccgatcat accgatgatc gccagcactt
     6781 taatcagcgc aaaccagaac tcggtttcac catataagcg cacgttcacc aggttaacgg
     6841 cgttgatgat aataaagaag gcggcagccc aaatccacgt tggaacatcc gggaaccagt
     6901 actgcatata gatgcccgca gcggtcagct ctgccattcc caccagcacg aacattaccc
     6961 agtagttcca gccagagagg aagcccgcaa acggtcccca gtatttatag gcaaagtggg
     7021 caaatgaacc ggataccggc tcttcgacca ccatctcgcc aagctggcgc ataatcagga
     7081 aagcgatgat cccggcgacg ccgtagccca gcaatacagc cggacccgcc atctgaatcg
     7141 ccgggccaat gccaagaaac agaccagtac caattgcgcc acccagcgca atcagttgaa
     7201 tatgacggtt atgtaatccg cgatgaagcg tcggctcttg attcgacgca gtatcttccg
     7261 atacggttga cgcgtttttc acgcctttcc cctgtgtgtc ttttttgttg aggggcacct
     7321 tttaacattt agtgcccatc gtcgcaagac acaatccaca cggttaaacg gggtatcctg
     7381 cttttatttc cgtacccgat gtcggtgcag ccacatcagc ttatacgccg ccgggataat
     7441 aaacagcgac agcaaaggtg cggtgatcat gccgccaatc atcggtgcgg caatccggct
     7501 catcacctct gaaccagccc ccgttcccca cagaatcggc agcagaccgg cgataatcac
     7561 cgccaccgtc atcgctttcg ggcgcacgcg caggaccgcg ccgtgatata acgcctcatc
     7621 cagcttctgc tcgctgaatg tttgtggatt attcaacgac ggctcggcct ctatggcgtg
     7681 acgtaaatac atcagcatca ccacgccaaa ttcggcggcg accccggcga gggcgataaa
     7741 gccagtgccc gtcgccacgg aaagatgaaa gcccatccac cacagcaacc agatgccgcc
     7801 caccagcgca aacggtacgc tgctgataat cagcaacgct tcgcccaccc gacggaacgc
     7861 cagatacaac agcacgaaga taatcatcaa cgtcatcggc accatgagtt taagcttatg
     7921 gttggcgcgc tccagtagct cgaactgccc ggagaatgcc acgctggtgc ccggttttaa
     7981 ctgcactttt tcagctatcg ctttttgcaa atcgtgaacc accgacacca tgtcacgatc
     8041 gcgggcatcg atataaatcc agctcgtcgg gcgcgcattc tcggttttca gcattgacgg
     8101 tccggtagag actttaatgt cggccacgtc tgccagggtg atttgctgct tcatcggcgt
     8161 cagaatcggc agctggcgca gtgcctgcgg actatcgcgc cagctttgcg gataacgcag
     8221 attaattgga taacgggcaa ttccttccac cgtttcgcca accatcgccc cgcccaccgt
     8281 agaagtcaca aacaactgca catccgccac cgtcataccg taacgcgcgg ctttttcacg
     8341 gttaatctca acgttgatat agcgcccacc ttccagacgc tcggcaagcg cagaagccac
     8401 gcctggtacc gttcgtgcca cctcttcaat ttgctccgcc atcgcgtcga tatccgccag
     8461 cacagtgccg gaaactttaa ttccgatggg gcttttaatg ccagtcgaga gcatatcgat
     8521 acggttacga attggcggca cccacagatt cgccagcccc ggcagacgca cggtgttatc
     8581 cagttcctca atgattttgt ccatcgtcat gcctggccgc cactgctcct gcggcttaag
     8641 ctggatggtc gtttctacca tctccagcgg agcagaatcg gtggcggttt ccgctttccc
     8701 ggttttccca aatacccgcg ccacttcagg tacgctcata attagcttgt cggttttttg
     8761 cagcatactc gccgcctctg ctgcggaaat ccccggcagc gtcgatggca tatacaacaa
     8821 gtcgccttca ttgatctgcg gtaaaaattc cccgccaact ttattgagcg gccagagaac
     8881 cgtcagcacc gaaagcgccg ccaccagcag cgtggttttc ggccagtgca gtactttcag
     8941 caacagcgga tgataaacac gaatcaaaaa gcgattgagc gggttactgc tttccggcgg
     9001 aattttgcca cggatccagt agcccatcag gatcgggatc actacgatcg ccagcagcgc
     9061 cgcacccgcc atcgcatacg ttttggtgaa cgccaacggg ccaaacagac gcccttcctg
     9121 cccttccagg gtgaagatcg ggataaacga caacgtgata atcagcagac tgataaatag
     9181 cgccggaccc acttcaacag atgcatcggt gatcacctgc cagcgcgttt tattatccag
     9241 cgtggcgtca ggatgctggt gctgccactc ttccagccgt ttatgtgcat tttcgatcat
     9301 gacgatagcg gcatcgacca tcgccccgac ggcaatcgca atgccgccca acgacataat
     9361 attggcattc agtccctgga agtgcatgac aataaaagca atacacaacc ccagcggcaa
     9421 cgaaataatc gccaccagcg ccgagcgcac atgccagaga aacagcgcac agactaccgc
     9481 caccacaata aactcttcca gcaacttgcc gctgaggttg tcgatggcgc ggtcaatgag
     9541 ctggctgcga tcgtatgttg taactatctc cacgccttcc ggcagactac ttttcagcgt
     9601 ttccagtttg tccttcacgg cggcgatcac ttctcgggcg tttttgccgg atcgcaggat
     9661 caccaccccg cccgccactt caccttcgcc attcagttcg gcaatgcccc ggcgcatctc
     9721 cgggccaatc tggaccttcg caacatcacg cagataaacg ggcacgccat tttcactggc
     9781 ttttaaaacg atgtgattaa agtcgtcgag cgtttgcaga tagccgctgg cgcgcaccat
     9841 atattccgct tccgccagtt cgatcgacga accgcccgct tcctggtttg aagcatccag
     9901 cgcgcttttt acttcggcta gactgatgcc atattgcgcc aggcgctggg gatcgataac
     9961 cacctgatac tctttcacca cgccgcccac cgacgccact tccgcaacgt cagggatggt
    10021 ttttagctca tatttgagaa accagtcctg caatgagcgt aaatcggcca gatcgtgctt
    10081 accgctgcga tccaccagtg catattcata gatccagcca acacccgtgg catctggtcc
    10141 cagctcggcg ctgactcccg caggcagctt accctgtacc tggttgaggt actccagcac
    10201 ccgcgaacgc gcccagtacg gatcggtacc atcttcgaaa atgacataca cataagagtc
    10261 gccaaactgt gagaaaccgc gcacagtctt cgcgccaggc accgacaaca tggtggtggt
    10321 tagcggataa gtcacctgat tttcaacgat ttgcggtgcc tgaccgggat agctggtttt
    10381 aataatcacc tgcacatcgg agagatccgg cagcgcatcc actggcgtgt taatgatggt
    10441 ccaggtgccc cagatgctca gaaacaacgc gcccatcagc accaaaaaac ggttcgccac
    10501 cgagcgacga ataatccatt caatcattgg ttattccctc aatgcgcatt ggtagcactt
    10561 tcagagcgca tccgctccag tgcgccagaa atattggctt cggaatcaat caggaacagg
    10621 ccgctggaaa ccaccttttc accttccgcc agaccagagt gtaacgcggt gacgccttgc
    10681 gatgcctgga aaacggcaac gcgtttcggt acaaagcgtc cgtcggcatc aacggtaatc
    10741 acccgctgtt cgttgccggt atcaatcagc gcctgtgacg gaatgagcag catcggttcg
    10801 ctggcggtat tgagttgcaa ccaggcgttc attcccggtt ttagcgcctc gtcggcgttg
    10861 tcgacttcca gacgcagctg cagcgtgcgg gtcgcggcat ccacgccagg tagcagcgtc
    10921 catttgcgga tggtgagtgt tttatccggt cgcgccggaa cggtgagggt aaactgcgag
    10981 gcatctttca ccagccaggc gatggactcc gggatcgcag cagtgaccca caccgggtcc
    11041 ataccctgaa ttttcgcgac cacgttatct ttggcgatat tcattcccgc gcgcagatca
    11101 aacgcggtga tcacgccatc aatgggcgct ttgagcgtaa agcgagtctg gattttttgc
    11161 gtggcgatca ggcggcgaat atccgcctcc ggcattcccg ccagtcgcag ccgctcaaga
    11221 atgccttcag tctgggtcgc cgtaccgccg gtttcgcgca gcagtaaata ctcactctgc
    11281 gcttccaccc agtcaggaat ggtcaggtcg agaagcggtg tgcccttttg tactttatcg
    11341 cccacggtaa gcggatacac cttgtcgata aacccggcag cgcgagcctg cacaatggca
    11401 tactgatact cgttgtaact gacattcgcc gggaaactct gggcaaaagt cagcggcccg
    11461 cgcgtgacgg tagccgtttt cacccccaga ttctgcgtct gagtcgggtc aatgcgcaca
    11521 ccagacgcag aactctcttc atcggcatat ttcggcacca gatccatatc cataaacggc
    11581 gatttacctg gtttatcgaa ccgcgtattg gggtacatcg ggtcgtacca gaataagatt
    11641 ttacgttctg cggtcgacgt tttttctgcg ggtggttcag cctttgcaaa ccaggtaaaa
    11701 cctgccgcag aaataatacc gcccgcgatc atgctgccga taataagcgc gatttttttc
    11761 atctcattaa acctgggtta ctggctgact ttaatatcct gtaataaaga aaggttgccc
    11821 tgctggacaa aattaaacgc cactttgtcg ccagttttaa tttcactcat tttcgtctgc
    11881 ggagtgatgg taaagcgcat ggtcatctcc ggccagttca cggcagcaat cggatcgtga
    11941 tggatggtga tttttttgct ttccagatca atacccttta ccacgccagt ggcgctaata
    12001 acctgcggtt gtacttcgct catggtttca tgatgatgtt cgttagcctg ggcattaaag
    12061 ccaataacgg taaacagact gaacattgcg acttgcagtg cttttttcat ttattctctc
    12121 ctggagttaa aagtcatact ctaaataatt cgagttgcag gaaggcgaca agcgagtgaa
    12181 tccccaggag cttacataag taagtgactg gggtgagcga acgcagacgc agcacatgca
    12241 acttgaagta tgacgagtat attgctgtca accgccacct aaggcggtat acaaagaaat
    12301 ttcgttaacc tgacgggcat aattcaggtc gagtaaagtt tgtcgggttg caaataaaga
    12361 acgctcggca tccagcactt ccagataact taccgcgccg tgctgatata atgcccgcgc
    12421 ccgttgcaaa gtaatttgca gcgacgccag ataacgctgc tgggcgctga tttgatcgtt
    12481 caggctttga cgtaatgcca gcgcatctgc cacttcttta aaggcgttct ggattttctg
    12541 ttcataattc actaccgact gctgctggcg aatttcggcg atatccagat tggcttggtt
    12601 gcgtccggca ttaaaaatgg ggatctcaat tttaggaata aaattccaca tcccgctgct
    12661 agcgttaaat aatgaagaca gatcgctgct ggcggttgat attccgctgg tcaggctgat
    12721 agacgggaaa aatgccgcac gcgcagcgcc aatattggca ttagccgcca ttaacgcgtg
    12781 ttcagcttcc ataatatccg ggcgctgcaa taagatttgc gacgacaatc ccgccggtaa
    12841 tttaacgctt tgcaggctgt cgctgtttac tgtctgcgct tgcggcagct tgccgtagct
    12901 tcccaataac agttgcaatg cattattcgc ctgcgccagt tccccctgac gtttagcgat
    12961 gtcgctgcgg gtactttcta tcaccccgcg agcctgttcc agcgccagaa cattactgct
    13021 acctgtcaac agttgttttt cgacaaacgc atatgactgc tgataattac gcagcgtttc
    13081 ttcggctatt tgcaattgcg catacgccag ttgctgattg aaatagcttt gcgcgacatt
    13141 agaaaccagc agaatatgca ccgcgcgctg agcttcctca gtggctaaat aattttgtcg
    13201 ctcggcttcg ctcatgttct ttaagcgacc gaaaaaatcg aggtcaaagc tggcgttaag
    13261 gccggtcgaa aactcccgtg tcgtggcggt attcccttta agattaccgc tccagctgcc
    13321 gctgccctcg ccattgagct gtgggtagcg gtcggcatcg gtcagacgat attgcgcccg
    13381 tgcctcctgc actttcagcg tcgccatgcg caaatcccga ttattcacca gcgcctcgct
    13441 aatcagcgtc ttcacctgat tatcaacaaa aaaggtgcgc cagcccgcgt tctgatagtt
    13501 atctgctgcg ttaaccaggc cgttctggct gagtgagaac tgctgcggca cgggcattgc
    13561 cggacgctga taatccggtg ccagtgaaca accggttagc gcaagggcca cacaaaatgg
    13621 cagaagttta caaggagaca taggctcata atttctggtg attttatgcc gccaacttta
    13681 ctcgccaggc tctgattttc cggtgacaga aaaatgacaa aattgtcatt ttgccaataa
    13741 gcgattgcca tctgatcccg ctactctaga attgcccggg caacatgcgg aggaaatatg
    13801 aaactgttga ttgtcgaaga tgaaaagaaa accggagaat acttgaccaa agggttaacc
    13861 gaagccggtt ttgtggtcga tttggccgac aacgggttga atggctacca tctggcgatg
    13921 accggtgatt atgatctgat aatcctcgat attatgctgc cggacgtgaa cggctgggat
    13981 atcgtgcgca tgttgcgctc cgccaataaa gggatgccga ttctgttgct taccgcgctt
    14041 ggcaccattg aacatcgcgt caaggggctg gagttggggg ccgatgacta tctggtgaag
    14101 ccgttcgctt ttgctgaact gctggcgcgg gtgcgcactc tcctgcggcg cggggcggcg
    14161 gtgattatcg aaagtcagtt tcaggttgcc gatctgatgg tcgatctcgt cagccgcaaa
    14221 gtcacccgca gcggcacgcg catcactctg accagtaaag agtttactct gctggagttt
    14281 tttctccgcc atcagggcga agtgctgccc cgctcgctta tcgcctcgca ggtgtgggac
    14341 atgaatttcg acagcgacac taacgccatt gatgtggcgg tgaagcggct acgcggcaaa
    14401 atcgacaacg actttgagcc gaagctgatt cagaccgtgc gcggcgtggg ctatatgctt
    14461 gaggtgccgg atggtcagta agccatttca gcgcccgttt tcgctggcaa cccgtctgac
    14521 cttttttatc agcctggcca ccatcgcggc gtttttcgcc tttgcatgga tcatgatcca
    14581 ctcagtgaaa gtgcattttg ccgagcagga tattaatgat ttaaaagaaa ttagcgccac
    14641 ccttgaacgg gtacttaatc atcctgacga gacgcaagcc cgacgcttaa tgacgctgga
    14701 agatatcgtc agtggttatt ccaacgtgtt gatttccctg gcagatagcc acggcaaaac
    14761 ggtgtatcac tcccccggtg cgccggatat ccgcgagttt acgcgtgacg ccatacccga
    14821 taaagacgct cagggtggcg aggtgtatct cctttccggc ccgacgatta tgatgccagg
    14881 ccacggtcac gggcatatgg aacacagcaa ctggcggatg attaacttgc cggttggccc
    14941 gttggtggac ggcaaaccga tttatacgct ctacatcgcg ctttcgatcg attttcatct
    15001 tcattacata aatgatttga tgaataaact tattatgacc gcatctgtca tcagcatcct
    15061 gatcgtcttt atcgtgctat tggcggtaca taaaggtcac gcgccgatcc gcagcgtcag
    15121 ccgtcaaatc cagaatatca cctcgaaaga tctcgacgtt cgccttgacc cgcagaccgt
    15181 gcccattgag ctggaacagc tggtactgtc gttcaaccat atgatcgagc gtattgagga
    15241 tgtctttacc cgccagtcca atttctcagc ggatatcgct cacgaaattc gcacgccgat
    15301 taccaatctc atcacgcaaa cggaaatcgc cctcagccag tcgcgcagcc agaaggaact
    15361 ggaagatgtg ctctactcta atctcgaaga gctgacgcga atggcgaaaa tggtcagcga
    15421 tatgctgttt ctcgctcagg ccgataacaa ccagctaatc cccgaaaaga aaatgctcaa
    15481 cctggcggat gaagtcggca aagtgttcga ttttttcgag gcgttagcgg aagatcgcgg
    15541 cgtggagttg cggtttgttg gcgacaaatg tcaggttgcg ggcgatccgc tgatgctgcg
    15601 tcgggcgtta agcaacctgc tttctaatgc cctgcgttat acgccaccca gtgaggctat
    15661 tgtagtgcgc tgccagacgg tcaatcatca ggtgcaagtt tccgtcgaaa accccggtac
    15721 gcccattgcg cccgagcact taccgcgatt gtttgaccgt ttctatcgcg ttgacccttc
    15781 ccgccagcga aaaggtgaag gtagcggtat tgggctggcg atagtgaaat cgattgttgt
    15841 cgcgcataaa ggcacggttg cggtaacgtc agatgcgcgg gggacaaggt ttgtgattat
    15901 gctgccggag agagagtgat tattaagttc aaattcaggg cgtggtgcat cacctcctgg
    15961 tattgttgat caacaaatta gagaaaaact tattaaacat atgaaatatc gaaggctttt
    16021 atttttcaaa gtttcacgag atgtttgtat atgaaactta gttaaatctg tgtttcttcc
    16081 aattgtagat caca
//
LOCUS       DAZGSP010000115        16062 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZGSP010000115 DAZGSP010000000
VERSION     DAZGSP010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.116"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..317)
                     /locus_tag="ACHK74_004145"
     CDS             complement(<1..317)
                     /locus_tag="ACHK74_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP0222869.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWA"
     gene            complement(468..665)
                     /locus_tag="ACHK74_004146"
     CDS             complement(468..665)
                     /locus_tag="ACHK74_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP0222870.1"
                     /translation="MLIQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(891..1712)
                     /locus_tag="ACHK74_004147"
     CDS             complement(891..1712)
                     /locus_tag="ACHK74_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP0222871.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESEKQGVPVFRNCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            complement(1709..2083)
                     /locus_tag="ACHK74_004148"
     CDS             complement(1709..2083)
                     /locus_tag="ACHK74_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP0222872.1"
                     /translation="MCIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVAFIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMLEGQVKK"
     gene            complement(2097..3146)
                     /locus_tag="ACHK74_004149"
     CDS             complement(2097..3146)
                     /locus_tag="ACHK74_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0222873.1"
                     /translation="MRVLLRPVLVPELGMVLLKPGRESMSAFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLAVWMIDVIRHAISGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPVEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMKRPKHRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            complement(3148..3426)
                     /locus_tag="ACHK74_004150"
     CDS             complement(3148..3426)
                     /locus_tag="ACHK74_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222874.1"
                     /translation="MAQDTKLHNSDNSAAPASRHGRRSHAFKSDWFQHDPCTEEQAEW
                     LIQCYRRRGYEFQKDLSFDRRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            3562..3819
                     /locus_tag="ACHK74_004151"
     CDS             3562..3819
                     /locus_tag="ACHK74_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFP0222875.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRSGK"
     gene            3825..4124
                     /locus_tag="ACHK74_004152"
     CDS             3825..4124
                     /locus_tag="ACHK74_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0222876.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGNVIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDGRKVFIVGIYHGGQSYETDFL"
     gene            complement(4327..4842)
                     /locus_tag="ACHK74_004153"
     CDS             complement(4327..4842)
                     /locus_tag="ACHK74_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222877.1"
                     /translation="MSEINYQEGHETAGQAKPVAWRYRYVKKDVTDFQGKPWAGDWKY
                     VPTKEDCNDRPNYEIQALFIGPPVPVTSEGLVKAVRFYEQVKRENPPVETGAWKDAVD
                     WVLREACCAAILGKADNPPASGNQVSELAMWVKRLVSQLKKAKPDCKLPDKAMDYLKR
                     NGLINEEDILR"
     gene            complement(4839..5015)
                     /locus_tag="ACHK74_004154"
     CDS             complement(4839..5015)
                     /locus_tag="ACHK74_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222878.1"
                     /translation="MADFGSTKYNVSFEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGDE"
     gene            complement(5008..5190)
                     /locus_tag="ACHK74_004155"
     CDS             complement(5008..5190)
                     /locus_tag="ACHK74_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222879.1"
                     /translation="MRKFKIIIETGIAGGDFEDVFEVDDDATPDEIHDEAKEIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            complement(5338..5643)
                     /locus_tag="ACHK74_004156"
     CDS             complement(5338..5643)
                     /locus_tag="ACHK74_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP0222880.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHRAEEALKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSERN"
     gene            complement(5640..5921)
                     /locus_tag="ACHK74_004157"
     CDS             complement(5640..5921)
                     /locus_tag="ACHK74_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0222881.1"
                     /translation="MILRQCVGTMKVKSIGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGVPRREIAEKFGMKLRAVNNYVYFDRRIQA"
     gene            complement(5954..6715)
                     /locus_tag="ACHK74_004158"
                     /pseudo
     CDS             complement(5954..6715)
                     /locus_tag="ACHK74_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            complement(6749..7210)
                     /locus_tag="ACHK74_004159"
     CDS             complement(6749..7210)
                     /locus_tag="ACHK74_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP0222882.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISESEANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSIPPIRRQLASPKRPQGPTPIELLKQEYERRKAAGFV"
     gene            complement(7203..8240)
                     /locus_tag="ACHK74_004160"
     CDS             complement(7203..8240)
                     /locus_tag="ACHK74_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP0222883.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     LKEDNGNVSQTAKSPAERKRAQREREKLRKYDADCHDESRRVTHLSRQVTTDKDTDTE
                     LNPTHNARMRESAPTGESNGASLQTAEPEFLDGLSEPIGKFSMTTVWQPSPDFRQRAA
                     VWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVS
                     HAASGGTASRAVQKIRAAREQWERENGFISNGNGLEAVGTYGGGVFEPMDPEERGCAV
                     ETLDCSDWRDD"
     gene            complement(8309..8734)
                     /locus_tag="ACHK74_004161"
     CDS             complement(8309..8734)
                     /locus_tag="ACHK74_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP0222884.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTSDAVEKIQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAVAIAWGTLTNSGGQPGNAVVVH"
     gene            complement(8718..8990)
                     /locus_tag="ACHK74_004162"
     CDS             complement(8718..8990)
                     /locus_tag="ACHK74_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0222885.1"
                     /translation="MLDSTRKKIRQKYTQAEIGRYMGGAQQTVWQWFSFGVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVEYQANTQPEAGVIHENQA"
     gene            9099..9500
                     /locus_tag="ACHK74_004163"
     CDS             9099..9500
                     /locus_tag="ACHK74_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0222886.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVTGHPLHWFLLPPEEGEQMFTPNTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            9528..9719
                     /locus_tag="ACHK74_004164"
     CDS             9528..9719
                     /locus_tag="ACHK74_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP0222887.1"
                     /translation="MSTALSPIVSEFETIEQENSYNEWLRAKVASSLADPRPAIPHDE
                     VIAEMENLIAQIAVTNRSE"
     gene            9719..10006
                     /locus_tag="ACHK74_004165"
     CDS             9719..10006
                     /locus_tag="ACHK74_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0222888.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHARRQFPFSI"
     gene            10280..10432
                     /locus_tag="ACHK74_004166"
     CDS             10280..10432
                     /locus_tag="ACHK74_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP0222889.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKKFKTEAGARRW
                     LARNTN"
     gene            10928..11782
                     /locus_tag="ACHK74_004167"
     CDS             10928..11782
                     /locus_tag="ACHK74_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP0222890.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKAGAGRGNPMMTTAHNRAQAVFLC
                     VMHSHIQIMVGRAGQPKGWPVSVVTGISTPVRLTTLMVVESLGGELFKLTIEAAIMAT
                     IPALSHPDVTIENGRAVTTSIAIAEFFGKRHERVLDKIRNLDCSAKFTEHNFVSSEYT
                     DSTSRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEKELRQNNAPSPDKM
                     IHGDGRTLVIRLDEHGNIKFTETVPDGAMVCTLDTFQFYLEKQGWTLVNRSAIKNMTV
                     EQLLKIHC"
     gene            11793..11981
                     /gene="dicB"
                     /locus_tag="ACHK74_004168"
     CDS             11793..11981
                     /gene="dicB"
                     /locus_tag="ACHK74_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP0222891.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLKLGIFGGQQ"
     gene            11978..12166
                     /locus_tag="ACHK74_004169"
     CDS             11978..12166
                     /locus_tag="ACHK74_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP0222892.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYASKESCHQARDEQKISGE
                     CLPLNKVSLYLNNEIPAG"
     gene            12259..14703
                     /locus_tag="ACHK74_004170"
     CDS             12259..14703
                     /locus_tag="ACHK74_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP0222893.1"
                     /translation="MSTDKEEIALYYEAKNDKVRKRLGIKGGFYWRTAKKLSVAISRG
                     VVAMDDAGFDEEDFKKPVRVNLPIVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPS
                     PSVQDKPASTDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREEL
                     QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRALRISRTPSGANAGGGILTDRGEGFVHDDASVE
                     RDVAAGVLARSMDIDIYNPHPAHAKRIEEIVSENKPPFSVFRDKFIAMPGHLDYSRAI
                     VVASVKEAPIGIEATPHRVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTKE
                     GKQDDEEKPQPSGAMADEQATTEAVEPDTTEHNQDTQSMNAQPQINSVDAKYQKLRAE
                     LYEARKNIPPKNPVDADKLLAASHGEFVEGISDPNDPKWVKGIETRDSVNHNQQETEQ
                     KGHKAEQHSPNAQQNEPETKQPEPVAQQEPEKVCTACGQTGGGNCPDCGAVMGDATYQ
                     ETFNEESQDEAREKDPEEMESAGLPNKECTEGDQHANGNNETGETANLLIKVNGHREI
                     TSTSRLWHHLMIDLETMGKNPDAPIASIGAVFFDPQTGEQGPEFSKIIDMGTCGGTVD
                     ISTIEWWLQRSGEARAAILADRIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNVI
                     LRRSYERQEIPCPWRYTNDRDVRTMVALGLVMDFDARNVTTFEGERHNALHDARYQAK
                     YVSAIWQKLFPNQADF"
     gene            14762..14965
                     /locus_tag="ACHK74_004171"
     CDS             14762..14965
                     /locus_tag="ACHK74_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFP0222894.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRNWLIKNGLPFYTNRSGKPIV
                     SRELFTCRNTLPPREAEPDFGAI"
     gene            14965..15990
                     /locus_tag="ACHK74_004172"
     CDS             14965..15990
                     /locus_tag="ACHK74_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0222895.1"
                     /translation="MGRRRKNPEHEKLPPKVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNHHDHAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVL
                     ADSVKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPVPVFIAMEIAYLCAARISDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWTPRLQSVIEKAKQLPKSAYVISNQYGNRYMYKGFNEMWVEARNLAGQIS
                     GIATDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKISPTLDVPLPQN
                     IPTKYSK"
BASE COUNT         4134 a         4201 c         3673 g         4054 t
ORIGIN      
        1 gcccagataa tatggttcag cacactgaag cgttcacgca tcatgatttc gatatcagat
       61 gccaggcgat ggccacagaa caggtaaaga cttccggcag gtttcagcac ccgccagaac
      121 tgcgccagac actggtccag ccacttcagg taatcatcgt cgcccttcca ctggttatcc
      181 cagccctcag gcttcacttt aaagtacggc ggatccgtga ctatcaggtc aacagaattt
      241 tcgggtaacg accggataaa ttccaggcag tcggcgttga ttaactcaca actggatatt
      301 tttacagtgt taagcatgga tcattaagcc tgtctctgat aggctcattc tgcttttgcg
      361 caaagcagtg ggcctgaggt ttgcttgtga tccagacgca tgagcagatg gctggtgagt
      421 gcccctaaca cccaccagcc gcccatttac cacaaataaa aaagccttca tgactgaagg
      481 cgtctgtaac aaccgaactg atagtctgcc agacccgcca taacaagctg ggtcagtatt
      541 aactggcagc gttcgcgtga aaggtaagta ttctgcgcaa tctccccgac tgtcgccggt
      601 tcggtgacgc ttaattcatt aaacaccact ctggcggttt cggtcatatc ctgctgtatc
      661 agcatgtctt tttccctttt ccggttaacg tgacacacca ataactcttg tcaaaaaagc
      721 cagcaagctg aaagaccggt attcaccgcc accagcgcgt ttactgtact ggcgtgattt
      781 cagtcataaa aaaacccgcc tggcgacggg tgtaaaaatc ttctaacgtc aggcataaaa
      841 cgcccatcgt tagggcaaat ttaccacaga ttcgggaaaa atcaacaaag ctatctggtc
      901 acctttttca gttgttgttc tgcccatgct tcttcaatat caaactgcac caccagcgta
      961 tcgtaaaaac gtttaactgt ttttttccat gtatcaagag atatggcatc ggttacatta
     1021 catatggcat taaatgcctc cgttgaaggt aatctttcat agccacgccc accacaacgc
     1081 tggcagtttc tgaaaaccgg cacaccctgt ttttccgact cttcacgatg aatggcaaca
     1141 ccgcgcccac gacaatcttt acaggcggtg gaaacctccc cctttccgcc acactccgga
     1201 caggcaactt ttaccacctc cctgactttt ttccattcct cccagtaaga cggatacacg
     1261 ccttttgtgc actttgccca cactggcggc ttaccatccg gatactggat cttgtttgta
     1321 aaaacctcgc tttcaataaa tttttttccg tgacagcagg ggcactgttt tttgctcgcc
     1381 gcgctacggg cataatcttc aaacgcatac gaagccataa tacgcatcac tgccggtttt
     1441 atttctgccg ggagttttct taacgccgcc acgcgatcac accgactgag tgcatattct
     1501 gtcagcaatt ctgttgcccg ctctctgtca ttcatactaa tgcccatttt cccaaggaac
     1561 gcagaaaacc ccatctcagc ccgattctgt gtcatgccct gcgcggccat cacatcagtg
     1621 atactcagcg catcttttga cgttgaggcc gatgcatcag tcaggccggg ggattttggg
     1681 gagtagtatt tcggtaaatc ttccagtttc attttttgac ctgcccttca agcattatgg
     1741 ggtaaatctt cacccccaga cgtccaccag atactggctg accacgtaca atattgattt
     1801 catcaaactg ttcatcgtcg ataagcagtc ccgcatgcgt cagcgcatcc agcggtgctt
     1861 tcagaatgtt gtccaggtca cgacgacgct tatccggtgg ctctgcaata atttttattg
     1921 ccagccgtcc ggacaggctt aatttcagtc gctgctggcg aacaataaac gccactgccc
     1981 ggcgataacg ctccccggct tttgatacaa aatatgtgct gccacgacgt cgccagtaag
     2041 tattcaccgt cggcgggtaa ggcaaaacaa actctataca catcaatacc ccccttttac
     2101 ccaagcacac cggttgcaaa ggcgtgatca agaaaacgaa aaattaaatc aacctgagaa
     2161 ccatgctttt cttcgaacgc cagcggatcc gcatgaagct cgttatgatg ctcccgacac
     2221 agcggtagcg tgaaaatatc gtgggctttt gttcccattc ccccctgacc atgaccaatc
     2281 aggtgatgcg gatcgtcagc tggcttacca caacacgcac acggctgtgt ctttacccag
     2341 cgcgtgtatt tctcattaac ccaacggcga tgtttaggcc gcttcataaa cgattcagga
     2401 gactccggat caacggcgat actgacaacc gtttttttct gtggtggatt ttgttgctgg
     2461 tggacgtgaa gtggcagcgc aatatttttt gtgcgctgct tcagtatgct gatggctgtc
     2521 tgttctcccg gtacgatgtc actctcacgg tatacggagc ggattttttc caccggtaat
     2581 cccagcgaac gacgcgctac tgcctcaggt agtgcatcca ccacctgatt gcaggccgcc
     2641 caccaggata attcggccag cgataactcc ctctcctgcg taccgcttat tgcgtgacgt
     2701 ataacatcaa tcatccagac tgccagattc tgctgagcaa gttgatcgag tgattctgat
     2761 gtctggtcgc gcagctggtt gtcacagtgc cagcacaaca ccatcgcgcc ggtaccgtaa
     2821 cggtgaatga cggtttcgct gtgatgataa tcgccgtgtg gccactggca ggatttcacg
     2881 tgacgtaata accagtcaga cagtgcacct gcaccacctg ctgcacgaat aacccgctca
     2941 tcgctgaaaa atggcagtaa tgttttatcc tctgccagcg gctggcgaac ggcaggaacg
     3001 actccggacg gcagaccgcg catgtttttc ggttccggct ccaccaatat tctgccgtta
     3061 tggaatgctg acattgattc acggcccggc ttaaggagca ccatcccgag ttccggtacc
     3121 agaacaggtc gaagtaatac ccgcacgtta cctccagata cgctgctgga atgtgcggga
     3181 cggacgcggt gggcgttcgg aataagggag cctgacggag attatccagt gacgacgatc
     3241 gaagctgaga tctttctgaa actcgtaacc acgtctgcgg tagcactgga tcagccattc
     3301 ggcctgttct tcagtgcatg ggtcatgctg gaaccagtca gatttgaatg catgagaacg
     3361 ccgcccgtgc ctgctggcag gggcggcgga gttatccgaa ttgtgtaatt tggtatcttg
     3421 cgccatcggt tgtctctgct ggcgcagcag gtgccagttg ttcaggctgg cgtgcggcaa
     3481 tattgtctct gatttctgtt gtcgtcaaca ggcagcgtgc tatcatcgaa tagtgttcta
     3541 tcctactccg tgaggtttac catgcgtaca acccaacaat tcagcattac attaactaac
     3601 gagatggctg acatggtgcg cgcccgtgtg gcttccggtg cctatgcttc agaaagcgag
     3661 gtcattcgtg aagggcttcg cgcactgaat gagcgcgata aagcaatcga agcgtggtta
     3721 acgcattcag ccgccccctc tcttgattct atccgcgaaa acccaaacaa cggacgctcc
     3781 atttcacagg ttcgcgccgc gattcgatcc gggaagtaat ctgcatgaca tatgaagtca
     3841 tcattactcc tgaggccgaa caacaaataa tcaacctgca cagatatata acggagaaag
     3901 cagggaacgt cattgctgac aattatgcca atgcgcttct tgattatctt gatgggtttt
     3961 ctacattccc gcatcggggc aataaacgcg atgatattcg ccaggggatg cgggtaactc
     4021 atttccgcca cagaacgatt attgcttttg ccgttgatgg cagaaaagtc tttattgtcg
     4081 gtatctatca tggtgggcaa agttatgaaa ccgatttctt ataaactttt acccacatca
     4141 ttccggtgtt agaataaacc gtctgcccct ctcttactgc ggattcgtag gctatataaa
     4201 tcaaagatcc cggctcatgt ttgtggcggg atcttttttc ggcaatttat ccccagcggc
     4261 aaatcgaata caccaccagc gccaccgcca ttgcaattcc taccgttatg aacgcttcag
     4321 gccaggtcat cgtaaaatat cctcctcgtt tatcagtccg tttcgcttca ggtagtccat
     4381 cgccttatcc ggtaatttgc agtccggctt cgcttttttc agttggctga ccagccgttt
     4441 aacccacatt gctaattcgc taacctgatt gccggatgct ggtggattgt cggctttacc
     4501 cagaatggca gcacagcagg cctctctgag cacccagtca acagcatcct tccatgctcc
     4561 tgtttcgact ggcggattct cacgctttac ctgttcataa aagcgcacag ctttaaccag
     4621 tccttctgat gtcaccggga ctggcgggcc gatgaataag gcctgaattt catagttcgg
     4681 cctgtcgtta caatcctctt ttgtcggtac atatttccag tcaccagccc acggcttccc
     4741 ctgaaagtct gtaacgtctt ttttcacgta gcgatatcgc catgcaactg gttttgcctg
     4801 ccctgccgtt tcatgccctt cctgataatt aatctcgctc attcatcgcc ccactcatca
     4861 caatatgctt cgaccggagt ttttcctgct tcataatcat cacgccatgc ttcagcatca
     4921 gcagcactgc caccacgtaa ctctgcatag tccattaaca gttcatgcca ttcttcaaaa
     4981 ctgacgttgt atttagttga accaaaatca gccattttgt tcttcctctt cgtcttttat
     5041 ttcgtgatat gagtaattgc agtagttaaa gaaaatttct tttgcttcgt catgaatttc
     5101 atcaggtgtt gcgtcatcgt ccacttcgaa tacatcctca aaatctccac cggctattcc
     5161 cgtttcaata attattttga actttcgcat ttcactaccg ccctgccggg cggtctcctg
     5221 atgttctgag ggtgcagaaa tccctccggt taaggattaa attttattta cagtgctgat
     5281 tttaattatt cagttctgga ttatgctttc tctttcacct gccgtagttc ctggtaatta
     5341 atttcgctca ctggttgcct cctggaaaat caccgcatgt cccagtttct ctgccagtgc
     5401 cagttctgcc ttagcgcctg ccgaccgctg ccagcctttc agcatgtaaa ttgcatccac
     5461 acaacgaatc attgccatgc aaatatccat gtaatgtggc tgagtcagcc cgtccggaag
     5521 tactgccggg tttaaaacgg tatgcccttc ccgtttcagc gcttcttccg ccctgtgaaa
     5581 cgcctcgcgg ttgaaatttt catatcccgt catcggaccg gcgatataaa ttctcaccct
     5641 cacgcctgaa tcctcctgtc gaaataaacg tagttattca ctgcacgcaa cttcattccg
     5701 aacttttcgg caatttcccg tcggggtaca ccgcgctgat gcagttgccg cgccagctca
     5761 atatcacgct gtgaatattt tgccgacggg tgaaaatcac cacgtaacat catactgatg
     5821 cccagctccc gtgccttcgt tctcactgcc gcttcagttc gtccgataag tgcgccaatg
     5881 cttttcacct tcatagttcc cacacactgg cggagtatca ggatttcagc ccggcaccac
     5941 gtcttccagc cactcaccgc tgctgttctc tggtggcggt aatatcccgg agaatatccc
     6001 ggcacttgtt cagctcccgt agcgcggcgc agactcgctc tcacttctga acctgacctt
     6061 ttgcccggcg cagctcgcgg ttagccacat gcagcgatgg taaaatcaga ccatccggat
     6121 gctttctggt gaacgacggc tgtgactgca ctgtgaccgc cacactttcc gtttttattt
     6181 cttcctgtgt ttccgcttcc cgaactggta acgcaacacc tgctggctga ggaaaggctt
     6241 taccatcggt ttccgctacg gatgcagctt ccggctctgc cggtaaatca gcgcccggta
     6301 tgcagtaacg aaatttaccg ttctgattta cgcgtgccag gcgccccgtt gctgttacga
     6361 ccgccaacgt ggaagcaacc ttgcgaatgc taacaccgaa cttatccgcc agttcctcac
     6421 acgttttagc cccatcctga cagataaact caatcatcat gtccgcggta actttttgtt
     6481 cgacctcccc ggtcagcaca tccggtactt cagattgtgc tggctgctct tcggttaccc
     6541 cggattcacc tttaccagcc agaaaccagg tgtgacccgt tttatcaacg acgccatttc
     6601 ttttcagttc ccacaactcg ttcagcactt cttcacgact gataccaagt cgcgcagcaa
     6661 gttctatgga tgtggctttt cccattgctt gcagtgcgtc aaaaacggtt tccattaaaa
     6721 cgtcctccga caaaatcatt tctcaaattc aaacaaaacc agccgccttc cggcgctcgt
     6781 attcctgttt cagtaattca attggcgttg gcccttgcgg gcgcttcggc gatgcgagtt
     6841 gcctccggat cggcggaata ctcatcccgt taccaacatg ctttgcccat ttcgtcagtt
     6901 gccgttccgc aagtcgtttt aactcaccct gcgtcatctg gcgctcaatc cctctggtac
     6961 gcatttcgag gcagatgtgg tacagcacag gctgtggcca cgggtattta tcactcccgt
     7021 cgtatcgcca ggattcattg cgccagcgcc ggtactcttc catcacggca tccaccgtaa
     7081 gaccaaatgg atttgcctca ctctccgaaa tcagcgcaac aaactcagcc aggtccgggg
     7141 gccacgtttc acccgcccgg cagcggtcca tgcactgacg gcagacctgc cggatttgct
     7201 gttcagtcat cgcgccaatc tgagcaatcc agagtttcga cggcgcagcc ccgttcttct
     7261 gggtccatcg gttcgaatac acctccccca taagttccca cagcttccag gccgtttccg
     7321 ttgctgataa atccgttttc acgttcccac tgttctcgtg ctgcccggat tttctgaact
     7381 gcccgtgatg ccgtgccacc tgatgctgca tggcttaccc ccttgctgac tggttttacc
     7441 tgtgccctga cgtgctgcac gtggcgggca aatttctgct cccactgaac ctgcgtgaaa
     7501 accttcccct ccgccatcca gtaatcccgg aatgcggcaa gctcagcagg tgtaaattcc
     7561 ggctcaggca gagccatacc ccacactgct gcccgttgtc gaaaatccgg cgacggctgc
     7621 cagacagtgg tcatcgaaaa tttcccgatc ggttcgctca ggccgtccag gaattcaggt
     7681 tcggctgtct gcaacgacgc accattcgac tcaccggttg gagcactctc gcgcatgcgc
     7741 gcgttatgtg tggggtttaa ttctgtatct gtatctttat ctgtcgtgac ttgtcgtgac
     7801 agatgcgtga cacgtcgtga ctcatcgtga caatcagcat catatttccg cagcttttcg
     7861 cgctcccgct gcgctctctt gcgctctgcc ggggattttg ccgtttgcga aacgttacca
     7921 ttgtcctctt tcagcacctg acgtttttcc catccggaaa taaggtcacc atccagaacc
     7981 cgcccctgca ttgcatgcaa aattgaatca attacgtctt ccgtcacatc aagcgcactt
     8041 gctaaatctt ccgtcgtgac atcaatgtga ccacgtagtg acacgccgtg acatgtcgtg
     8101 acatttcgtg acgcgctcac cagaagatgg atatacactg ccatcactgt tgcgattggc
     8161 tgtcctgata ccctggcaat cgttcgccac ttggggtcat ttggcatgtc atgccacaat
     8221 ctgagccagg cattagccat actcacctct tctgataccg aactttaccc acgaacttcc
     8281 ggaagaaatc cggtataaat attgttggtc aatgcacaac aactgcatta ccaggctgac
     8341 caccactgtt agtcagggtg ccccaggcga tcgctacagc gacaaaatca tccacatctt
     8401 tcaccagccg atcccgtcgt tcgacgatct cccggtaata ttcagaactg tgactgcgca
     8461 tacgggccac cagcaaaggc ggcatcgcct tttcgatcgc cggtaacagc gcctgaattt
     8521 tttcaacagc atcagacgtg tccttctcca cccagcggaa aattttctgg gtattacgag
     8581 ccagggcttc cggatggctg tcgtcgtaca gttccgggaa cgtcatcccc agctcgaaat
     8641 aagtccgggc tatttcagct gctggaactt tctcaccgtc tggatacgcc caggcattca
     8701 ttgccatgcg gatgtgttca tgcttgattt tcatgaatca cccccgcctc tggttgtgtg
     8761 ttagcctgat actcgacagg taagccgtcg gtcggattag ggtaaatgtc tgggcgaatt
     8821 tcatgcgggg taacctccca cttcattagc tgacatagcg gaattacctg ctttggggga
     8881 acgccaaagc taaaccattg ccaaactgtc tgttgagcgc cccccatata acgacctatt
     8941 tcagcctgag tgtatttctg cctaattttt ttgcgagtgc tatctagcat tttgccctcc
     9001 tctaaaaact ataggcaaag gctacaataa aaaactgtac gcgatcaaca gttttttatt
     9061 gtgatgcttt taacagtatt tacctgtaaa attgaataat gatgagcgcc ctagaagtat
     9121 cgatgtacag aatcagcaag cttcttcagg aaactggatg gagccaggct gagctggccc
     9181 gtagaattgg tgtgacacaa caaactgttc aacaatgggt cagcggtaag gctacaccta
     9241 aagcctcaag tttggataaa ctggttgagg ttacagggca tccgttgcat tggtttttat
     9301 tgcctcctga agagggggag caaatgttca cccctaacac gatgaaaatt ggtcctcgcc
     9361 aacgcgaatt actccaggct tttagtgcgt tccctgagga ggaccaagaa aaaatgcttc
     9421 aagaaatcaa agacaaaaaa aaatcaatgg aagaaaccat tgcccggtgg ctggcggcac
     9481 aaaaaagccg ccgggcgtga ccacagtaca agaagaggag ttatgccatg agtacagccc
     9541 tttctccgat agtttcagaa ttcgaaacta tcgaacaaga aaacagttac aacgaatggt
     9601 tgcgcgctaa agtagcgtca agccttgcag accctcgccc tgcaattcca catgacgagg
     9661 taatagctga aatggaaaat cttattgctc aaattgctgt aactaacagg agcgagtaat
     9721 gttgcccatt ttatggctac catctgctcg cgatgatttg cgtcagatca taacttacat
     9781 cgccaaggag aacccaccgg cagcgcgtag acttaaaata cgtattgaaa catcggtatt
     9841 acctctatct gagcatccgt acctatatcc accaagcgaa cgggtttctg ggttgagaga
     9901 gatagtgacc caccctaact acataatcct gtacagagta gctgcttcaa gcattgagat
     9961 tgtaagcgtg acacatgctc gccgacaatt tcccttctct atctgagctg aactattttc
    10021 atactccctc tttcgaggga tttttttgct taaatgcaca attaaaaact gttgacacaa
    10081 aacagttttt aattgtagat tgcactcacc aacccactcc gccccacaga acgccaggca
    10141 atacttcgag ttacccggca gtggcaaggg gttaagtagc cagcccgagg cgtatgaaca
    10201 tgacggcggg aacactttgt ataacagcgc agcaggtttt tagttccgcg acccggcgtt
    10261 aagggtaaat gaggtcaaca tggatacgct caatcttggc aacaacgaat ctctggtatg
    10321 cggtgtgttc cctaaccagg acggcacgtt taccgcgatg acgtatacca aaagtaaaaa
    10381 gtttaaaacc gaagctggcg cgcgtcgctg gttagccaga aacactaact gattagcacc
    10441 agtaaaaaca ggttgccact ggttaattta ccctgaaaag tcagggtata acacgaaagc
    10501 gcacggcgaa gctctttccc ttagaaggct tgtcgttaga tttcttcgaa cgtgcgcttc
    10561 cggttgtggc aatccgcgaa atggcgcggc ggtaagtatg gctggggttt cctccattgc
    10621 tccagaaaat gcaccgggtt gtcaggttga ccatacgatt aagtgacagc cccgccacaa
    10681 tacccatgtg tagtctttgg tggcatcagt tctactccgt gactgctctg ccaccctttt
    10741 taaagtgaat tttgtgatgc ggtgaatgcg gctaagcgca cgcggaacag ttaaaacaag
    10801 cggtctttta ctggcgtaac agacatcaac taacaatccg gcgttaattg ttaactggtt
    10861 aacgtcacct ggaggcacca ggcactgcat caacaaagtt cacttcggtg atgaagggta
    10921 agagaaaatg ttgaatgtag ctattgaaaa ccagaatggg tggaattata gtgcacctgc
    10981 acctcataaa gcgggtgccg ggcgtggaaa cccgatgatg actactgcgc ataaccgcgc
    11041 tcaggcggtt tttttatgcg taatgcacag ccacattcag attatggtgg ggcgtgcagg
    11101 gcagccgaaa ggctggccgg tttcggtagt caccggtatt tccacccctg tacgtctcac
    11161 cacccttatg gtcgtggaaa gccttggtgg tgagttattt aaactgacta tcgaggctgc
    11221 catcatggct actatccctg ccctttctca ccctgacgta accatcgaaa atggacgcgc
    11281 tgtcactacg tctattgcga tcgctgagtt ctttggtaaa cgccacgaac gagtgttgga
    11341 taaaattcgc aatctggact gttcagcaaa attcactgag cacaattttg tgtcgagcga
    11401 atataccgac tcaaccagtc gcaaactccc aatgtaccaa atcaccaaaa acggcttcgt
    11461 tttcctggtg atgggcttca ccggcaaaaa agccgctgca tttaaagaag cctacatcgc
    11521 tgagttcgat cgcatggaga aagaactgcg ccagaataac gccccgtctc ccgacaaaat
    11581 gattcacggg gacggacgta ccctggttat ccgtctcgac gaacacggca atatcaaatt
    11641 cactgaaacc gttccggacg gcgcaatggt ctgcaccctg gataccttcc agttttatct
    11701 ggagaaacaa ggctggactc ttgtaaaccg gagcgcaatt aaaaatatga ctgtggagca
    11761 attactaaaa attcattgtt gaggacgcga taatggaaac gttattacca aacgttaata
    11821 cgtctgaagg gtgttttgat attggtgttc tgctcagtaa ccgggagttt actgaagatg
    11881 ccattaagat gagaaaatat gagccttatc ttctcaatga taattccata ctctccagaa
    11941 ttgcccttct taaacttggt attttcgggg ggcagcagtg acttctgcat ttgcactggt
    12001 aatgactgtt tttcttataa cgggtgagcc acagaatgtg attaccggaa tttatgccag
    12061 taaagaatcc tgccatcagg caagagacga gcaaaaaatt tccggtgaat gtctcccgtt
    12121 aaacaaagta tcgctgtacc tgaataacga aataccggct ggataacccg ccagccatat
    12181 taacgccata cccgttgatt aagtatgcca gcaatggcag ggattcgtac aaccttaaaa
    12241 tagttatgag gtttatcaat gagcactgat aaagaagaaa ttgcactgta ttacgaagcc
    12301 aaaaatgaca aagtcagaaa acgccttggg attaaaggcg gtttttactg gcgcacagca
    12361 aaaaaattat cggttgcaat atcacggggt gttgtcgcaa tggacgatgc tggatttgac
    12421 gaagaggatt tcaaaaaacc tgttcgcgtg aatttgccca ttgttaatga cctgccgcct
    12481 gaaggtgtgt ttgataccga attctgcaac cgctatgaaa aaggcggaga agatggcatc
    12541 acaatgatat ttatagcgcc ttccccctca gttcaggaca aaccagccag cactgacaat
    12601 accaacgtca atggcgaaga catggctgag attgaggata atatgctcct gccgatttcc
    12661 ggtcaggaac tgcccattcg ctggcttgcg caacatggca gcgaaaaacc ggtaacgcac
    12721 gtttcacggg aagaacttca ggcattacat atcgcacgag ctgaagaact gcctgctgtt
    12781 actgccctgg ctatttccca caacacaaag ctgctcgacc cgctggagat tcgcgacctt
    12841 cacaaactgg tacgcgacac agacaaagtt ttccctaatc ccgttaattc cagtctgggg
    12901 ttaatgactg cttttttcga agcatacctg gacgctgact ataccgatcg aggtctgctg
    12961 acaaaagagt ggatgaaagg aaatcgtgct ttacgcatca gccgcacgcc atccggcgct
    13021 aatgctggcg gaggaattct taccgatcgc ggtgaaggtt ttgtccacga tgatgcgtca
    13081 gtggaacgtg acgttgccgc tggcgttctg gcccgttcaa tggacatcga tatttacaat
    13141 ccacatccgg cacacgccaa acgcattgaa gaaatcgttt cagagaataa gccgcccttt
    13201 tctgtttttc gtgacaaatt catcgccatg cctggtcacc tggattattc ccgcgcgata
    13261 gtggttgcgt ccgtgaaaga agcaccaatt ggtatcgagg ctactcccca ccgtgttacc
    13321 gaatatctga acaaagtact gaccgaaacc gaccatgcca accctgatcc agaaatcgtg
    13381 gatattgcct gcggtcgctc ctctgctcca atgccgcagc gtgtaacaaa agaaggaaaa
    13441 caggatgatg aagaaaaacc gcagccatct ggcgcaatgg cagatgaaca ggcaacgact
    13501 gaagcagtgg aaccggatac aactgaacat aatcaggaca cgcagtcgat gaatgctcag
    13561 ccacagataa attctgttga tgcgaaatat cagaaactgc gtgcagaact ctatgaagcc
    13621 aggaaaaaca ttccacccaa aaatcctgtc gatgcagata aattactggc tgcttctcac
    13681 ggagaatttg ttgaagggat tagcgacccg aatgatccaa aatgggtgaa ggggattgaa
    13741 acccgcgatt ctgtgaacca taaccagcaa gaaacggaac agaaaggcca taaagcggaa
    13801 caacacagtc caaatgcgca acaaaacgag ccagaaacga aacagcctga accagtagcg
    13861 caacaggaac cggaaaaagt ctgcacagcc tgcggtcaga ccggcggcgg caactgtcct
    13921 gactgtggcg cggtgatggg cgacgcaaca taccaggaga cattcaacga agaaagccag
    13981 gatgaagccc gggaaaaaga tccggaggaa atggaaagtg ccggactccc gaacaaggag
    14041 tgcaccgaag gcgatcaaca tgccaatggc aataatgaaa caggcgagac agcaaatctc
    14101 ttaattaagg tgaacggtca tcgtgaaatc acatccacca gcaggttgtg gcaccatctg
    14161 atgattgacc ttgaaacaat gggcaaaaat cctgacgcac caatagcctc aataggcgct
    14221 gtatttttcg atccacaaac cggagagcag gggcctgaat tcagcaaaat aattgatatg
    14281 ggtacatgtg gcggcactgt agacataagc accatcgaat ggtggcttca acgctctggc
    14341 gaagcccgtg ccgccatttt agctgatcga ataccgcttg atgatgcgct tttacaatta
    14401 cgggaattta tagacgaaaa ctccggtgag ttttttgttc aggtctgggg aaatggagcc
    14461 aacttcgaca acgtgatttt acgccgttca tatgaacggc aggagatccc ctgcccgtgg
    14521 cgttacacca atgatcgcga tgtaagaacg atggtcgctc tggggttggt gatggatttc
    14581 gatgcccgca acgtcaccac atttgagggt gaacgccata atgccctgca cgatgcgcgt
    14641 taccaggcaa aatacgtttc agctatctgg caaaaactgt tcccaaatca ggctgatttt
    14701 taatgttcaa ccctgatcgc cgctaaccgc atatagttag cggcggttat gagatatagc
    14761 tatgagcagc ttatttttaa ccgaagatga attgctaata ttaacgggct gcaaatatgc
    14821 aagccaccag cgcaactggt taataaaaaa tgggcttccg ttctatacca atcgtagtgg
    14881 aaaaccgatc gtcagtcggg aactgtttac ctgtagaaac actttaccac cacgtgaggc
    14941 tgaacctgat ttcggtgcga tctaatggga agacgaagga aaaatcccga acacgaaaaa
    15001 ttaccgccaa aggtatatcc caataaatat agttatgtat ggaaaccaac atccagagaa
    15061 tctgtaacct taactgcaat caaggatggt ttagccgcat tatggaaaaa atatgaagaa
    15121 acggttaacc atcatgatca cgcaatgaca tttgggcgtt tgtgggaaaa atttctcgcc
    15181 agcgcctatt acagcgatct tagtcccaga acgcaaaaag attatctgca acatcaaaaa
    15241 aaactgctgg ccgtattcgg taaggtgctg gccgattctg taaaaccaga gcacatcaga
    15301 cgatacatgg acaaaagggg cgagcagagt aaaacgcagg caaaccatga aaaaagcagc
    15361 atgtcacgcg tttacagttg ggggtatgaa cgagggtatg taaaggctaa tccttgtgcc
    15421 ggtgtaagta aattcaaggc caaaaaccga gagcgctatg tgactgacaa agaataccag
    15481 gccgtattaa gcgtggctcc tgtgcctgtt tttatcgcaa tggaaattgc atacctgtgc
    15541 gcagcaagga tttccgatgt gttgtctctg aaatgggaac agatcggaaa cgacgggatc
    15601 tttatccagc aaggtaaaac aggaaagaaa cagataaaag cctggactcc ccgtctgcaa
    15661 tcagttatag aaaaagctaa acagctaccc aagtcagcct atgtaatcag caatcagtac
    15721 ggcaaccgtt acatgtataa aggatttaat gaaatgtggg ttgaggcaag aaatttggct
    15781 ggacaaatat cagggatcgc gacggatttt acattccatg accttaaagc caaggggata
    15841 tcagattacg aaggtagtag ccgggataag caacttttct ctggtcacaa gaccgaagga
    15901 caggtgctaa tctatgatag aaaagtaaag atctcaccca cactggatgt accattaccc
    15961 cagaatattc caacaaaata ttccaagtaa ttccaagtgt gattttcatc attgacttaa
    16021 tgatgtgtaa gtgattgaat tctggcggag agagggggat tt
//
LOCUS       DAZGSP010000116        15901 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZGSP010000116 DAZGSP010000000
VERSION     DAZGSP010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.117"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..324)
                     /locus_tag="ACHK74_004173"
                     /pseudo
     CDS             complement(1..324)
                     /locus_tag="ACHK74_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
     gene            680..1594
                     /gene="sitA"
                     /locus_tag="ACHK74_004174"
     CDS             680..1594
                     /gene="sitA"
                     /locus_tag="ACHK74_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFP0222896.1"
                     /translation="MLSIKKVTMLLGCLVLTCSIAFQASAAEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDAFIKYDPINAQTYQ
                     RNADTYKAKITQTLAPLRKQIAELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            1594..2421
                     /gene="sitB"
                     /locus_tag="ACHK74_004175"
     CDS             1594..2421
                     /gene="sitB"
                     /locus_tag="ACHK74_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFP0222897.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPGGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            2418..3275
                     /gene="sitC"
                     /locus_tag="ACHK74_004176"
     CDS             2418..3275
                     /gene="sitC"
                     /locus_tag="ACHK74_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFP0222898.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQLTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            3272..4129
                     /gene="sitD"
                     /locus_tag="ACHK74_004177"
     CDS             3272..4129
                     /gene="sitD"
                     /locus_tag="ACHK74_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFP0222899.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKSWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQASGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISILVSMTGVWLSFFIDSAPAPTIVVLFAVMFIMTFTVTSINARTKENA
                     EPRDLLSSD"
     gene            complement(4363..4620)
                     /locus_tag="ACHK74_004178"
                     /pseudo
     CDS             complement(4363..4620)
                     /locus_tag="ACHK74_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            4618..5031
                     /locus_tag="ACHK74_004179"
                     /pseudo
     CDS             4618..5031
                     /locus_tag="ACHK74_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
     gene            5372..5752
                     /gene="crcB"
                     /locus_tag="ACHK74_004180"
     CDS             5372..5752
                     /gene="crcB"
                     /locus_tag="ACHK74_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFP0222900.1"
                     /translation="MIKSLFAVIIGGSVGCTLRWLLSTRFNSLFPNLPPGTLVVNLLA
                     GLIIGTALAYFLRQPHLDPFWKLMITTGLCGGLSTFSTFSVEVFALLQAGNYIWALTS
                     VLVHVIGSLIMTALGFFIITILFA"
     gene            complement(6132..7325)
                     /locus_tag="ACHK74_004181"
     CDS             complement(6132..7325)
                     /locus_tag="ACHK74_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0222901.1"
                     /translation="MSSKIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISLTGSKTVVLWALGGYGVSFTLLGLGSVLM
                     ATGAVTTAVGLGILIIARIVYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLVIHPLAPVWMLMAAPVWAVVMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAMLLAAAMSMMQLGLSPALTRQFDTDTTTISQQVAWLLGLSAIAALIAQFVV
                     LRPQRLTPVALLLSAGVLMSSGLAIMLTEQLWLFYLGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLAASHTLGYGLCALLVPLASKTGVSIALIVTALFAAVLFTIVSACIWH
                     YRTIK"
     gene            7461..9185
                     /gene="iucA"
                     /locus_tag="ACHK74_004182"
     CDS             7461..9185
                     /gene="iucA"
                     /locus_tag="ACHK74_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFP0222902.1"
                     /translation="MILPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPAHLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESYAHTQQTIDARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RQEAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQVWCQALFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSVRTLSVKEVERGMRLARLAQTDGWQMLQARFPTFRVMQEDGWAGLRDL
                     NGNIMQESLFSLRENLLLEQPQSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATEWLDTIDEAQAENIFTREQLLRYFPYYLLVNSTFAVTAALGAAGLDSEANLM
                     ARVRTLLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            9186..10133
                     /gene="iucB"
                     /locus_tag="ACHK74_004183"
     CDS             9186..10133
                     /gene="iucB"
                     /locus_tag="ACHK74_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFP0222903.1"
                     /translation="MSGANIVHSGYGLRCEKLDKPLNLGWGLDNSAVLHWPGELPTGW
                     LCDALDQIFIAAPQLSAVVLPWSEWCEEPQALTLFGQVQSDIIHRSAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQIAYLERQLTSKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGVTHYLLLNEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            10133..11875
                     /gene="iucC"
                     /locus_tag="ACHK74_004184"
     CDS             10133..11875
                     /gene="iucC"
                     /locus_tag="ACHK74_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFP0222904.1"
                     /translation="MNHKDWDLVNRRLVAKMLSELEYEQVFHAESQGDDRYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLNADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNEMDIHQLLTAAMDPQEFARFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            11872..13205
                     /gene="iucD"
                     /locus_tag="ACHK74_004185"
                     /pseudo
     CDS             11872..13205
                     /gene="iucD"
                     /locus_tag="ACHK74_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016528830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
     gene            13231..15432
                     /gene="iutA"
                     /locus_tag="ACHK74_004186"
     CDS             13231..15432
                     /gene="iutA"
                     /locus_tag="ACHK74_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFP0222905.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSLGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAAFLQSGYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         3830 a         3997 c         4069 g         4005 t
ORIGIN      
        1 ccatttccgc gcagacgatg acgtcactgc ccggctgtat gcgcgaggtt accgactgcg
       61 gcctgagttt tttaagtgac gtaaaatcgt gttgaggcca acgcccataa tgcgggctgt
      121 tgcccggcat ccaacgccat tcatggccat atcaatgatt ttctggtgcg taccgggttg
      181 agaagcggtg taagtgaact gcagttgcca tgttttacgg cagtgagagc agagatagcg
      241 ctgatgtccg gcggtgcttt tgccgttacg caccaccccg tcagtagctg aacaggaggg
      301 acagctgata gaaacagaag ccactggagc acctcaaaaa caccatcata cactaaatca
      361 gtaagttggc agcatcaccg atcccttcat ttagtgactg cccctgggtt tttcatgctc
      421 tcctgcgtcc tagagaactt gccaataacc tacattattt ttgctttgcc attaccaatc
      481 gtcctgaaca agttcatttt aaagccaaag cgctgcccaa cttttatcct caataccaca
      541 tcgtcagcag gaaataacct aatccctgtg ttgggatttt ataatcgcta atgataatta
      601 tttacatttt atagcttctg ctataaaaca tagtagttgc tatgtttatt ctaattttgc
      661 tcactatagg tactaaatca tgctctcaat aaaaaaagta accatgctct tggggtgcct
      721 agtcctcacc tgctcgatag catttcaggc gagtgcagct gaaaaattca aggtcattac
      781 aacttttacc atcatcgcag atatggccaa aaacgtggct ggagatgctg cagaagtctc
      841 atccataacc aagcctggtg cagaaattca tgagtatcag cctacccctg gcgatattaa
      901 acgtgcgcag ggggcacaac tgattctcgc caatggtatg aatctggaat tgtggttcca
      961 acgcttttac cagcatctca atggggttcc agaagtaatt gtctcttcgg gtgtgacgcc
     1021 agtagggatc accgaagggc cctatgaggg caaacctaac ccccatgcct ggatgtcgcc
     1081 agataatgct ctgatttacg tcgataatat tcgtgatgcg tttataaaat acgatccgat
     1141 aaatgcacaa acctaccaac gtaatgccga tacttataaa gccaagatta cccaaaccct
     1201 tgcccccctg cgtaagcaga ttgcggaact ccctgagaat cagcgatgga tggtcaccag
     1261 tgaaggggct ttttcttatc tcgcacgcga tttggggcta aaagagcttt atctgtggcc
     1321 gattaatgcc gatcaacaag gaacaccgca gcaggtacgt aaggttgttg atatagttaa
     1381 gaaaaatcat atcccggcag tctttagcga gagtacgatt tccgataaac cagcgcgtca
     1441 ggttgcgcgt gaaaccggcg cgcactacgg tggtgtgctc tatgtcgatt ccctgagcac
     1501 agaaaacggc ccggtaccta cgtatatcga ccttctgaag gttactacca gtacgctggt
     1561 acagggaatt aaagccggaa aaagggagaa ataatgatgc aatctgcagg cattgtcgtt
     1621 aatgatgtta ctgtcacctg gcgtaacggg cacacagcac tgcgcgatgc atccttcaca
     1681 gtaccaggcg gatctattgc cgctctggtt ggggtaaacg gttccggaaa atcgacgttg
     1741 tttaaagcga ttatgggatt tgtgcgtctg accagcggga agatatctgt tctgggtatt
     1801 cccacacgac aggcgctaca gaaaaacctg gttgcctacg tccctcagtc agaagaggtt
     1861 gactggtcat ttcctgtact ggtggaagat gtggtgatga tgggccgcta tgggcatatg
     1921 ggtatgttgc gtatcgccaa aaagagggat cgtcagattg tcaccgatgc gctggaacgt
     1981 gtcgatatgg tggattttcg ccatcgacaa atcggcgagc tttccggtgg acaaaagaaa
     2041 cgcgtctttc tggcgagagc gattgcacag cagggtgatg tgatcctgct ggatgaacct
     2101 tttaccggcg ttgatgtgaa aaccgaagcg aaaatcatca gcctgttgag ggagttacgc
     2161 gcggaaggca aaacgatgct tgtctcaacg cacaatctcg gatccgtcac gacattttgc
     2221 gattatacgg tcatggtcaa aggcaccgtc ctggcaagcg ggccgacaga caccactttt
     2281 acagccgaga acctggagct ggcttttagc ggcgtactgc gccatgtcac acttaacggc
     2341 tcggaagaaa gcattattac tgacgatgaa cgcccttttg tggcacatcg accgtcagcg
     2401 gtgcagaggg aagaaagatg aacgtgctac tggaaccctt cagctatgaa tatatgctca
     2461 atgcgatgtg ggtctcggcg atggtcggcg gtctctgcgc tttcctgtca tgctatctga
     2521 tgctaaaagg ctggtcgctg attggcgatg cgctgtcgca ctctattgtc ccgggtgttg
     2581 cgggggcata tatgctgggg cttccgtttt ctctgggagc cttcttttcc ggcggactgg
     2641 cagcgggcag catgctgttt cttaaccaac tcactcgctt aaaagaagat gccatcatcg
     2701 gcctgatctt ctcgtccttt tttggcctgg ggctgtttat ggtgtcattg aacccgacat
     2761 ccgtgaacat tcagaccatc gtcctgggca atattctggc aatcgatccg gcggatatcc
     2821 tgcaactcac tatcattggc attctctcga tcatcgtgct ttttttcaaa tggaaagatc
     2881 ttatggtgac ctttttcgac gagaatcacg cccgtgctat tggattgcat ccgggcagat
     2941 taaaaatcct cttcttcacc ctgctgtcgg tttcgaccgt tgcggcgctg caaacggttg
     3001 gtgcctttct ggtgatctgt ctggtggtga ctcccggtgc aacagcatgg ttgttaaccg
     3061 atcgttttcc ccgcctgctc atgattgctg taactattgg cagtgtgacc agtttcctcg
     3121 gcgcgtgggt cagttacttt ctggacggcg cgacaggtgg aatcatcgtg gtggcgcaga
     3181 cactgctgtt tcttttagca tttgtctttg cgcccaccca tgggcttttg gcaaatcgtc
     3241 ggcgtgcgca taaagcgctg gaggatcgct catgatggca ttattactgg agccactgca
     3301 gttcaccttt atgagtcacg cactgctgat ttcactggta gtttccatac cctgtgcgct
     3361 gctgtcggtc tttctggtcc tgaaaagttg ggcactgatg ggtgacgcga tgagtcatgc
     3421 ggtttttcct ggcatcgtgc ttgcctggat tctggggtta cctctggcta caggggcatt
     3481 cgtcgccgga gtattctgtg cggtggcgac gggatacctg aaagacaaca gccgaatcaa
     3541 gcaagataca gtgatgggga ttgttttttc tggcatgttt gccgcaggtc tgatcttgta
     3601 tattgcagtc aaaccagacg tacatcttga tcatattctt ttcggcgaca tgctcgggat
     3661 aaccatcggc gatataatcc agacggtgat tattgccgga ctggttacac ttgttattag
     3721 cgtaaaatgg cgggattttt tgctgttcag ctttgattat caacaggcgc aggcaagcgg
     3781 tttgcatacg agatggctgc attatgggct gctgtgcatg gtctccctga ccattgtggc
     3841 gacgctgaaa gcggttggta tcattctctc tatttctctt ctcattgcgc ctggtgccat
     3901 tgcggtactc ctgacacaac gctttcatat tgcgctcctg ctggcgactg gcatatcgat
     3961 attggtctca atgactggtg tctggctgtc cttttttatc gacagcgcgc cagctccgac
     4021 aattgttgtc ttattcgcgg tcatgttcat catgacgttt accgttacca gcatcaacgc
     4081 acgcacaaag gaaaatgccg aaccacggga tttgttatca tccgattagc agaacactcg
     4141 gccagcaaca tccgcttatt ggcgatgcgc ttgaacgaat aatgcacaga cttatgattc
     4201 tgataagttt tgttgccaga gagttgctgc ggactggata gcaagtaatg tcgattctca
     4261 ggggtagcaa acagcgcagt aaaatgaggg aagtagtggc aaaacataga gaaaattggg
     4321 gggcgagggc cagtgatgta aaaaaccacg ctaagagttt aattaattgt ttgtattgaa
     4381 gtttaatgat cttaactcac ccttaagaat atcttccggt aacgaagacg tataatgccc
     4441 cagcatattt atatggctat gtatcagcag cgataatctt gccggatgct actcctcagg
     4501 agtttctccc gatttgcgaa gatgtgacaa cgcctcctgc atgtaaagtg tgtttcacag
     4561 cacaaccgca ttggtcacca gccccagagc acctagttga tcttcctgcc cttaacggta
     4621 gctgaagatg actgggctgg atggaaaatc ctgcatggtg cattgggaga gcagatagaa
     4681 ttggttggcg acgatctgtt tgtgaccaac gtaaagtata ttcagcgcgg cattgacgaa
     4741 aaccttgcca attcagcatt gattaaactc aaccagattg gttccctgag tgaaaccttt
     4801 gatgcggtac aactttgcca tgacaacaac tggggcacct ttatctcaca ccgcagtggt
     4861 gagacagtgg acagctttat cgccgatatg acagttgcaa tgcgcgcagg tcatctcaaa
     4921 acgggagctc catgccgggg tgagcgtatt gaaaaatata accagcttat gagaattgag
     4981 gatgaactgg gttcatcggc gcaatttgcc ggaaagtctg cctttaaatg acattattgt
     5041 gccaatcccc caggcttaca cgtcggggtt attctggagg aatgatgaaa gctcacgttt
     5101 ttgttatttc tatgcttact ggcattgtcg tcacctatgc cgtgttactt cttggttgct
     5161 tgttcatcga taaaacgctc cccactgtag atgttgtcat tctgtcactt gtcgtggggg
     5221 cttctgctca gcaattgtcg agggtattaa tgtcaataaa tagaaccttc ccttatcttg
     5281 catgtagtca aaggatataa attaacaata tcagtcaaat gagaacatat tctgatattg
     5341 ttacgataaa aaattatttt atgaggggta catgattaaa tcactttttg ccgtcattat
     5401 cggcggttct gttggatgta ctctgcgctg gttactgtcc acacgcttta acagtctttt
     5461 tccaaatcta cctccaggta cactggtggt gaacctcctt gccgggttaa ttattggtac
     5521 tgcgttggcg tactttttac gacaaccaca ccttgatcct ttttggaaat taatgataac
     5581 aacgggactt tgtggaggtc tgagtacttt ctctacattt tctgttgaag tattcgcatt
     5641 actccaggcg ggaaattata tatgggcttt gacgtctgta cttgttcacg tcattggctc
     5701 cctcatcatg acagctttag gcttttttat cataaccata cttttcgcat gagaattcat
     5761 taacgttaca ggtttatcaa ctaatgtgac gcttaggata gaatttcgct ccatggtatg
     5821 cgtttgcagt cattacagtc tccatgacga ctgtaatgac tgcaaacgca ttacctgaca
     5881 tatcagtata aacaatgaat taacctcttg gcgtattttt tacatcactg gccctcaccc
     5941 cccaaaattc tctctgaaaa attgttgcgg ttaacgttgc caatgtatcc acccatttca
     6001 gaagacgatc tggcatcctg gtggcatgct gcgatgtgct tcaggtgagt gcactgggcg
     6061 gtcagcacag gcggtttcga cagcgaaatt gcacaggacg ttatgccggc tgtgagtaca
     6121 acatcatagc cttacttgat tgtacggtaa tgccaaatac atgcagacac tatggtaaat
     6181 aatacagcgg caaataatgc cgtcacaatc agcgcgattg agacacctgt tttcgacgcc
     6241 agtggcacca acaatgcgca cagcccatag ccaagtgtgt gactggccgc aagccagccc
     6301 gcgcctgcgc cgtcggccag cttatcattc agtaaaagtt gataagcggg tgttgccaga
     6361 gcggccccaa atgacagcac tgcacagccc aggtaaaaca accatagctg ttcagtgagc
     6421 atgatagcca gaccactact catcaacacc ccggcactca ggagcagagc cactggagtc
     6481 aggcgctgtg gacggagtac cacgaactgc gcgataagcg cagctattgc ggacagcccc
     6541 aacaaccacg ccacctgttg gctaatagtg gtggtatcag tatcgaactg gcgagtaagg
     6601 gctggcgaaa gtccaagctg catcatgctc attgccgcag ccagtaacat tgcgcaaagc
     6661 agataaggca ggcaatcccg cttcaggctg acgctcttgc gctccggtgt gggctgtggt
     6721 ggtgtgccgg gcaaccgcag aagcatcacc acagcccaca ccggagctgc catcagcatc
     6781 cacactggcg ccagagggtg aatgaccaac attgccgccg cgcacagcgg cccgaatagc
     6841 ctgccgcagc tcaggccgga gctgatggtt gccagagcgg ccatgcgatt cccttctccc
     6901 gctctctgca atgcccagac ctgacaggct ggcaccattg ctgacacggt cagaccgtag
     6961 acgatccggg cgatgattaa tattcccaac cccaccgctg ttgttacggc accggtagcc
     7021 atcagcacgc ttcccagccc aagcagggta aagcttacgc catatcctcc cagagcccac
     7081 agcaccacag tcttactgcc agtaagggaa atttgctttc cccaccatgg ggaagatggc
     7141 agaaacagca ttgagcccag catcagcagg gcagcccata cagacagact cagatttgtc
     7201 tgtatgacca gaacagggag tacaaccagc agaccgttct gtccgatacc gagaagaccg
     7261 gcactgaacg ccagtggcca gcaggacagg gttttttggg gcgtatcttc gatcttagat
     7321 gacattgtaa tgtttcaaca ccatgtatta attgtgttta tttgtaaaat taatttttct
     7381 gacaataaca tttctcgttg ataatgagaa tcattattga cataattgtt attattttac
     7441 tgtgtaggag ctgtttgatt atgatcctgc cctctgaaaa atccgccaca gatgtggctg
     7501 cgcagtgctt cctgaatgcc ttgattcgtg aaaccaaaga ctggcaactg gcggaatacc
     7561 cgccagacga attgattatc ccgctggacg aacagaagtc gctccatttc agagtggctt
     7621 atttctcccc aacccagcat caccgctttg catttcctgc tcatctggtc acggcatcag
     7681 gcagttatcc tgtcgacttt accactctct cccggcttat tattgataag ctccggcatc
     7741 aactttttct gccagttccc ctctgcgaaa ctttccacca acgcgtgctg gaaagctacg
     7801 cccatacgca acagacaatt gatgcccgcc atgactgggc catcctgcgt gaaaaagcgt
     7861 tgaattttgg cgaggctgag caggcactgc tgacaggaca cgctttccac cctgcaccta
     7921 agtctcatga accgtttaac cgccaggaag ctgaacgata cctgcctgac atggcacctc
     7981 acttcccgct gcggtggttt tcggtggata aaacgcaaat cgctggtgaa agtctgcatc
     8041 ttaaccttca acagcggttg acgcgatttg ccgcagaaaa tgcgccccag ttactcaacg
     8101 aattaagtga caatcaatgg ctgttcccgc tgcacccgtg gcagggagaa tatcttttgc
     8161 agcaagtgtg gtgccaggca ctttttgcta aaggacttat cagagactta ggcgaggccg
     8221 gcacgtcgtg gctgccgacc acctcttccc gctccctcta ctgtgctacc agccgcgata
     8281 tgatcaagtt ctccctgagc gttcggctga ccaactccgt ccgtactctg tctgtgaaag
     8341 aggtggagcg aggaatgcgc ctggcacgtc tggcgcaaac cgacggctgg cagatgctac
     8401 aggcccgctt ccctactttc cgggtaatgc aggaggacgg ctgggccggg ctgcgcgatc
     8461 ttaacggcaa catcatgcag gaaagtttgt tttccctgcg tgaaaacctg ctgctggagc
     8521 agccgcaaag ccagaccaac gttctggttt ccctgactca ggccgcaccg gatggcggtg
     8581 attcgctgct ggtttcggcg gtaaaacgcc tgagcgatcg cctcggtatc actgtgcaac
     8641 aggccgccca cgcatgggtc gatgcttact gtcagcaggt gctgaagcct ctgtttacgg
     8701 ctgaagcgga ttacggcctg gtgctgctgg cgcatcagca aaatattctt gtgcagatgc
     8761 ttggggatct gccggtcgga tttatttacc gtgactgcca gggcagcgcg tttatgcctc
     8821 acgcgacaga gtggctcgat accattgacg aggcgcaggc ggaaaatatt ttcacccgtg
     8881 agcagttgct gcgctatttc ccttattacc tgctggttaa ctccactttt gccgtaactg
     8941 ctgcgctggg tgctgccggg ctggacagcg aagcgaatct gatggctcgt gtacgaacat
     9001 tgctggctga agtgcgtgac caggtgactc ataaaacctg tctcaactat gttctggaaa
     9061 gtccgtactg gaacgtaaaa ggtaactttt tctgttatct gaacgatcat aacgagaaca
     9121 ccatcgttga cccttcggtg atctattttg atttcgctaa cccgctgcag gctcaggagg
     9181 tctgaatgtc tggggcaaac attgttcaca gcggatatgg actgcgctgt gaaaaactcg
     9241 acaagcctct gaatcttggc tgggggctgg acaatagcgc ggtgttgcac tggcccgggg
     9301 agctgccaac agggtggctg tgcgacgcgc tggatcagat atttatcgcc gcaccacaac
     9361 tttcagcagt ggttcttccc tggtccgaat ggtgtgagga gccacaggcg ctgacgcttt
     9421 tcggacaggt acaaagcgac attatccatc gttccgcttt ctggcagtta ccgttatggc
     9481 tgagttctcc ggcaaaccgg gcctccggtg aaatggtttt tgatgcagag cgtgagattt
     9541 atttcccgca gcgccccccc cgtccgcagg gtgaagttta tcgtcgttac gatccacgca
     9601 ttcgcaggat gctgagtttc cgcattgccg atcccgtttc tgatgcagaa cgtttcactc
     9661 gctggatgaa cgatccgcgc gttgagtatt tctgggagca aagtggctca ctggaggtac
     9721 agatcgccta tctggaacgc cagctcacca gtaaacatgc attcccgctg attggttgtt
     9781 tcgacgatcg gccgttcagt tatttcgaaa tctactgggc ggcggaagac cgcattggtc
     9841 gccactattc gtggcagccc tttgaccgtg gcctgcatct gctggttggt gaacagcaat
     9901 ggcgcggagc ccactatgtg caaagctggc tgcgcggggt gacacattac ctgctgctga
     9961 atgagccacg tacgcagcgc acagtactgg agccacgcac cgataaccag cgcctgttcc
    10021 gccatcttga gcctgcggga taccggacaa ttaaagagtt cgacttccca cataaacgct
    10081 cgcgcatggt gatggcggat cgtcatcact tcttcacgga ggtcggtctg taatgaatca
    10141 caaagactgg gatttggtca accgccgcct ggtggcaaaa atgttgtctg agctggagta
    10201 tgagcaggtt ttccacgcgg aatctcaggg cgatgaccgc tactgcatta acctgccggg
    10261 agcacaatgg cgcttcatcg ctgaacgtgg tatctggggc tggctctgga ttgatgctca
    10321 aactctgcgc tgcgcggacg agccagtact ggctcagacg ctgctgatgc agctaaagca
    10381 ggtactgtca atgagcgatg caaccgttgc tgagcatatg caggatttgt atgccacgct
    10441 gctgggcgac ctgcaactac tgaaagcccg tcgcgggctg agcgccagtg acctgattaa
    10501 tcttaatgcc gaccgcctgc aatgcctgct gagcggtcat cctaaattcg tttttaataa
    10561 aggtcgccgt ggctggggta aagaggcgct ggaacgatat gcgccagagt atgccaacac
    10621 cttcagactg cactggctgg cggtaaaacg tgaacatatg atctggcgct gtgataacga
    10681 gatggatatt catcagttgt tgacggccgc aatggatccg caggagtttg cccgcttcag
    10741 tcaggtctgg caggaaaacg gactggatca taactggctg ccgctgccgg tacatccgtg
    10801 gcagtggcag caaaaaatcg ctaccgactt catcgctgat tttgccgaag gcaggatggt
    10861 gtctctcggc gagtttggcg accagtggct cgcccagcag tcgctgcgta ccctgaccaa
    10921 cgccagtcgc cggggcgggc tggatatcaa gctgccgctg accatctaca acacctcatg
    10981 ctaccggggg atccctggca gatacatcgc tgccggacca ctggcttcac gctggctaca
    11041 acaggttttt gcgaccgacg ccaccctagt gcaaagcggc gcagtgatac ttggtgaacc
    11101 ggctgcaggc tatgtctccc atgaaggcta tgccgcgctt gcccgggctc cctatcgcta
    11161 ccaggaaatg cttggtgtta tctggcggga gaatccgtgc cgctggctga aaccggatga
    11221 aagtccggtt ctgatggcaa cactgatgga gtgcgacgaa aacaatcagc cgctggcagg
    11281 cgcatatata gaccgctccg ggctggacgc tgaaacctgg cttacgcaac tgttccgggt
    11341 ggtcgtggtt cctctgtatc acctgctttg ccgctacggt gtcgcgctta ttgcacatgg
    11401 acaaaatata actctcgcca tgaaagaggg ggttccacag cgtgttctgc tgaaagactt
    11461 ccagggcgat atgcgactgg tgaaagaaga gttccccgaa atggactctt tgcctcagga
    11521 ggttcgtgat gttacatccc gcctgagtgc ggactactta atccatgatt tgcagacggg
    11581 tcacttcgtg acagtactgc gttttatttc gccactgatg gttcgtcttg gcgtacctga
    11641 aaggcgattt tatcaactgc tggcagcagt gttgagtgat tacatgaaaa aacatccaca
    11701 aatgtcagag cgttttgcgc ttttctcact tttcaggcca caaatcattc gcgtggtgct
    11761 gaacccggta aaactgacct ggcctgatct ggatggcggc agccgcatgc tgccgaatta
    11821 ccttgaggat ctgcaaaatc cgctgtggct ggtaactcag gaatatgaat catgaaaaaa
    11881 agtgtcgatt ttattggtgt agggacaggg ccatttaatc tcagcattgc tgcgttgtca
    11941 catcagatcg aagaactgga ctgtctcttc tttgatgaac atcctcattt ttcctggcat
    12001 ccgggtatgc tggtaccgga ttgtcatatg cagaccgtct ttctgaaaga tctggtcagt
    12061 gctgttgcac ctacaaatcc ctacagtttt gttaactatc tggtgaagca caaaaagttc
    12121 tatcgcttcc ttacaagcag actacgtaca gtatcccgtg aagagttttc tgactacctc
    12181 cgctgggctg ctgaagatat gaataacctg tatttcagtc ataccgttga aaacattgat
    12241 ttcgataaaa aacgtcgatt gtttctggtg caaaccagcc agggagaata ttttgcccgc
    12301 aatatctgcc ttggtacagg aaaacaacct tatttaccac cctgtgtgaa gcatatgaca
    12361 caatcctgtt tccatgccag tgaaatgaat cttcgtcggc cggaccttag tggaaaacgg
    12421 ataaccgtgg ttggtggagg acagagtggt gcagacctgt tccttaatgc attacgcggg
    12481 gaatggggag aagcggcgga aataaactgg gtctcccggc gtaataattt taacgcactg
    12541 gatgaggctg cttttgctga tgagtatttt acacctgaat atatttcagg cttctccgga
    12601 ctggaggaag atattcgcca tcagttactg gatgagcaga aaatgacatc ggatggcatc
    12661 actgccgatt ctttactgac catttatcgt gagttgtacc accgttttga agttctgaga
    12721 aaaccaagaa atatccgtct gctacccagc cgctcggtaa caactctgga aagtagtggt
    12781 cctggctgga agttgctgat ggagcatcat ctggatcagg gcagggagag cctggaaagt
    12841 gatgtggtga ttttcgccac aggttaccgt tctgcgttgc cacaaatact tccctcactg
    12901 atgcccctga tcaccatgca cgataagaac acctttaaag tgcgtgatga cttcactctg
    12961 gaatggagtg gcccgaaaga gaacaacatc ttcgtggtca acgccagtat gcaaacccat
    13021 ggcatcgccg aaccccagct cagcctgatg gcatggagat ctgcacgtat tcttaatcgc
    13081 gtaatgggac gtgatttatt cgatctcagt atgccgcccg ccctgattca gtggcgcagc
    13141 ggcacctagg gaaaacgcag ccggaggctg cttctttaac tcgctacaca gcatctttgg
    13201 gctgattttt tccgcccgta tggaggaata atgatgataa gcaaaaagta tacgctttgg
    13261 gctctcaacc cactgcttct taccatgatg gcgccagcag tcgctcaaca aaccgatgat
    13321 gaaacgttcg tggtgtctgc caaccgcagc aatcgcaccg tagcggagat ggcgcaaacc
    13381 acctgggtta tcgaaaacgc cgaactggaa cagcagattc agggcggcaa agagcttaaa
    13441 gacgcactgg ctcagctgat ccctggcctt gacgtcagca gccggagccg caccaactac
    13501 ggtatgaatg tgcgtggccg cccgctggtc gtgctggttg acggcgtgcg tctcaactct
    13561 tcacgtaccg acagccgaca actggactct atagatcctt ttaatatcga ccatattgaa
    13621 gtgatctccg gtgcgacgtc cctgtacggc ggcggcagta ccggtggcct gatcaacatc
    13681 gtgaccaaaa aaggccagcc ggaaaccatg atggagtttg aggctggcac caaaagtggc
    13741 tttagcagca gtaaagatca cgatgaacgc attgccggag ctgtctccgg cggaaatgag
    13801 catatctccg gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac
    13861 ggcgatgcca ccttgcttga taacacccag accggcctgc agtactccga tcggctggac
    13921 atcatgggaa ctggtacgct gaacatcgat gaatcccggc agcttcagtt gatcacacag
    13981 tactataaaa gcctgggcga cgacgattac gggcttaatc tcgggaaagg cttctctgcc
    14041 atcagaggga ccagcacgcc attcgtcagt aacgggctga attccgaccg tattcccggc
    14101 actgagcggc atttgatcag cctgcagtac tctgacagcg cttttctggg acaggagctg
    14161 gtcggtcagg tttactaccg cgatgagtcg ttgcgattct acccgttccc gacggtaaat
    14221 gcgaacaaac aggtgacggc tttctcttcg tcacagcagg acaccgacca gtacggcatg
    14281 aaactgactc tgaacagcaa accgatggac ggctggcaaa tcacctgggg gctggatgct
    14341 gatcatgagc gctttacctc caaccagatg ttcttcgacc tggctcaggc aagcgcttcc
    14401 ggagggctga acaacaagaa gatttacacc accgggcgct atccgtcgta tgacatcacc
    14461 aacctggcgg ccttcctgca atcaggctat gacatcaata atctctttac cctcaacggt
    14521 ggcgtacgct atcagtacac tgaaaacaag attgatgatt tcatcggcta cgcgcagcaa
    14581 cggcagattg ccgccgggaa ggctacatcc gccgacgcca ttcctggcgg ctcagtcgat
    14641 tacgacaact tcctgttcaa cgccggtctg ctgatgcaca tcaccgaacg ccagcaggca
    14701 tggctcaact tctcccaggg cgtggagctg ccggacccgg gtaaatacta tggtcgcggc
    14761 atctatggtg ctgcagtgaa cggccatctt cctctaacaa agagtgtgaa cgtcagcgac
    14821 agcaagctgg aaggcgtgaa agtcgattct tatgagctgg gctggcgctt tactggcaat
    14881 aatctgcgta cccaaatcgc ggcctactat tcgatttctg ataagagcgt ggtggcgaat
    14941 aaagatctga ccatcagcgt ggtggacgac aaacgccgta tttacggcgt ggaaggtgcg
    15001 gtggactacc tgattcctga tactgactgg agtaccggag tgaacttcaa cgtgctgaaa
    15061 actgagtcga aagtgaacgg tacctggcag aaatacgatg tgaagacagc aagcccatca
    15121 aaagcgacag cctacattgg ctgggcaccg gacccgtgga gtctgcgcgt gcagagcacc
    15181 acctcctttg acgtgagcga cgcgcagggc tacaaggtcg atggctatac caccgtggat
    15241 ctgctcggca gttatcagct tccggtgggt acactcagct tcagcattga aaacctcttc
    15301 gaccgtgact acaccactgt ctgggggcag cgtgcaccac tgtactacag cccgggttac
    15361 ggcccagcgt cactgtacga ctacaaaggc aggggccgaa cctttggtct gaactactct
    15421 gtgctgttct gaccggtatt cctttacaac aaaggtacgc tgatatcaac atggccgctg
    15481 acagccaagt tgatatcata taatacacga cataatctgt agtcagggag gatagactct
    15541 ttactgacta cagattatgt cgtgttccgt gctcatttcc tcaaaaaaat acaagaaaag
    15601 aattagtatt ctaacaaaaa gtgaaataaa ttgtatcaaa ctccctcttt taatcctgtt
    15661 gagtaaatca gcttttgcaa taggattgaa agagtgtaag tggaatctct tccggatact
    15721 cgttaccacc gtggctagaa tatctacggc tgcgggccag taatagatta agtgataaca
    15781 gctgtctaga aatgtagggg cgtatccact aatcataatc attgtgagat aaatattacg
    15841 gggcatttta taatgaataa aatatactct ctaaaatatt gtcatattac caataccatg
    15901 a
//
LOCUS       DAZGSP010000117        15809 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZGSP010000117 DAZGSP010000000
VERSION     DAZGSP010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.118"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..416)
                     /locus_tag="ACHK74_004187"
     CDS             complement(<1..416)
                     /locus_tag="ACHK74_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP0222906.1"
                     /translation="MKRLQAFKFQLRPGDQQECEMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYRKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYR"
     gene            424..855
                     /gene="tnpA"
                     /locus_tag="ACHK74_004188"
     CDS             424..855
                     /gene="tnpA"
                     /locus_tag="ACHK74_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFP0222907.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRAYFSNVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPG"
     gene            complement(871..1059)
                     /locus_tag="ACHK74_004189"
     CDS             complement(871..1059)
                     /locus_tag="ACHK74_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFP0222908.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(1063..1422)
                     /gene="yeaR"
                     /locus_tag="ACHK74_004190"
     CDS             complement(1063..1422)
                     /gene="yeaR"
                     /locus_tag="ACHK74_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFP0222909.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(1595..2233)
                     /gene="leuE"
                     /locus_tag="ACHK74_004191"
     CDS             complement(1595..2233)
                     /gene="leuE"
                     /locus_tag="ACHK74_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFP0222910.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(2360..3283)
                     /gene="dmlR"
                     /locus_tag="ACHK74_004192"
     CDS             complement(2360..3283)
                     /gene="dmlR"
                     /locus_tag="ACHK74_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFP0222911.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            3386..4471
                     /gene="dmlA"
                     /locus_tag="ACHK74_004193"
     CDS             3386..4471
                     /gene="dmlA"
                     /locus_tag="ACHK74_004193"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFP0222912.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            4722..6332
                     /gene="yeaV"
                     /locus_tag="ACHK74_004194"
     CDS             4722..6332
                     /gene="yeaV"
                     /locus_tag="ACHK74_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFP0222913.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            6364..7488
                     /gene="yeaW"
                     /locus_tag="ACHK74_004195"
     CDS             6364..7488
                     /gene="yeaW"
                     /locus_tag="ACHK74_004195"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFP0222914.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGGGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            7544..8509
                     /gene="yeaX"
                     /locus_tag="ACHK74_004196"
     CDS             7544..8509
                     /gene="yeaX"
                     /locus_tag="ACHK74_004196"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFP0222915.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNHYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8563..9678)
                     /gene="rnd"
                     /locus_tag="ACHK74_004197"
     CDS             complement(8563..9678)
                     /gene="rnd"
                     /locus_tag="ACHK74_004197"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFP0222916.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKVFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(9760..11445)
                     /gene="fadD"
                     /locus_tag="ACHK74_004198"
     CDS             complement(9760..11445)
                     /gene="fadD"
                     /locus_tag="ACHK74_004198"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFP0222917.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(11650..12231)
                     /gene="yeaY"
                     /locus_tag="ACHK74_004199"
     CDS             complement(11650..12231)
                     /gene="yeaY"
                     /locus_tag="ACHK74_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFP0222918.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(12271..12966)
                     /gene="tsaB"
                     /locus_tag="ACHK74_004200"
     CDS             complement(12271..12966)
                     /gene="tsaB"
                     /locus_tag="ACHK74_004200"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFP0222919.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(13024..14934)
                     /gene="yoaA"
                     /locus_tag="ACHK74_004201"
     CDS             complement(13024..14934)
                     /gene="yoaA"
                     /locus_tag="ACHK74_004201"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFP0222920.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            15066..15410
                     /gene="yoaB"
                     /locus_tag="ACHK74_004202"
     CDS             15066..15410
                     /gene="yoaB"
                     /locus_tag="ACHK74_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP0222921.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKHGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         3860 a         4149 c         3871 g         3929 t
ORIGIN      
        1 ttgcggtagc gcatccagcc cagtttcggc agaaaaatac ggctgttttc ctgatcgagc
       61 ttaacgccct gcgggtagcg gaatgcatca ttctgtcccc gctttttgaa tcggggaaaa
      121 gcagcccgct tccggaagaa gtttttgtaa gcccgctcaa ggtctttcag tgactgttgc
      181 aatggctgtg agggagaatc tttaagccat tgcgtttcag tggcattttt ccactcaacc
      241 agccaggaag ccattttcct gtaagggatg tatttattac cggcctcatg attctcattc
      301 tgaagcgcca gagcacgatt gaaaacgaaa cgacatgcgc ctgcgaagcg cctcatttca
      361 cactcctgtt gatcacctgg tcttaactgg aatttaaatg cctgtagtcg tttcacaggc
      421 caaatgatac tttggtctgt gaaaaaagaa accgatatcc ggcgtggaag gcattgtgtt
      481 ttcctgatgc atgttcacct ggtctttgtc accagataca gacgtcagat ttttgaccat
      541 gacgcgacag aaaaactacg cgcttacttt tcaaatgtat gtgctgattt tgaagctgaa
      601 cttgttgaaa tggatggcga accagatcac gtccatttgt taattaacta tcctcccaaa
      661 ctggcgatat ccagtctggt aaacagcctc aaaggcgtat cgggtaggtt actgcgacga
      721 gatcgaccag atattgcagt caggtattac tacaaaggcg ttttgtggag tcctggctat
      781 tttgccagta gctgcggagg tgcgccaata tccgtcatcc gccaatacat tgaacaacag
      841 caaacacctg gttaggtgga aaaccgcgcc ttatatcccc gccctgaagg acggggtttt
      901 acggcgcacc ggataagaac gcacggtatt caccagatct tttatcactt cagccgccac
      961 ttctggcacc agcaaagtca tcggcgtctc tgtttcataa tcgacagaaa cgccattact
     1021 gttattggtg acggtcacgg tatacgttgc tttgcccatg attcatttcc cgttatgaat
     1081 gactttccgt tgttgcgcac cttccatcag gacttcagga gccacgaaga agtcaatgtt
     1141 gaaataagta tcgtcagtca tggcttcaat gttgtgccac ttttctggag ggaacaccgc
     1201 aaactgcccc gcttcgataa ggatcacctg atcaggctct gcactgtgtt catcagcgta
     1261 gccgagatat ttgaccgccc catgcataac ggaaaggcgt gggtaaaccc ccgggcgcgt
     1321 tcctttatca agatgacgtt cgaatattcc ggcaggcgca gtttgtttat tccagaaagg
     1381 cgttgagcgc gtatgaatat aattctgtgg gatttgcagc atcctattcc ctccttcggg
     1441 gaatgcgctg aaaacggttt attccagccg tttcagggta cgcctgataa tttgcatttt
     1501 aaataccatt tattggttac tttttagcac catatcagag aaaaatcagg gaggattaca
     1561 agtgggaagc ccgcgacaac gcgggctgaa tgtatcagga ttgcagcgtc gccagtcggg
     1621 cggcgaaacc cacgaacatc aaaccaatca gtgagttgcc aactttagcc agtttctttt
     1681 tggtacgtat gtactgcgtg acaaaagcac cagaaataat caggaagctc aaatagcaga
     1741 aactcaccag ttccagcgtc gtcgccagaa taaagaatga aattcccgta tgtggggcat
     1801 taacatcgat aaactgtacg aaaaacgaca catagaacaa aatggctttc ggattagtca
     1861 ggctcaaaat taacgcgcgt ttaaaaatgg ccccgtattg gggctcatcg gatttggcct
     1921 cgttattttt acccttcagg gtcgcgtaga gaattttact ccccagatag agcaaataaa
     1981 acgcaccaag ataacgtaca atgttgaata atatcggggt ggtcttaatt aatgtcgcca
     2041 ctccagccca tgccagaaac atcaatactg catcgccaat aaatacaccg caggccgcaa
     2101 gataaccgcc tttcataccg ctactgacgc tatttttgag tacaaacagg gtatttggcc
     2161 ctggcaccaa cacaataaaa atggccccaa ccagataggt ccagtaattc agaaccccgt
     2221 attcagcgaa cacattaacc tctttaatta tctttcgatc atgcgcgatt aaaggtgaat
     2281 atgctaacca atctgtagcg gcttagaaag gagaaaatca ggttttaacc cgatatcaac
     2341 ccgataattg aatcattaat caggcatgct ccagtgaaaa attcgggtag tgctcggcaa
     2401 aatactggcg taaaaattct accgttatcc gcactttcgc tgacgtcgcc agccttgaaa
     2461 cataaacggc ccagacgttc gctggctggt aatattccgg tagcacttgc actaaatgac
     2521 cactggcaat gttttcgcta acatcccacc aggagcgcag agcaatcccc tgcccatcga
     2581 gacaccactg atggacaatt tctccatgat tagacgacag tgggccggtg actttaatcg
     2641 catggtggcc ttctttattg cgtaattgcc agacaccaaa aggatgatcg cgctctttaa
     2701 tgaccaggca cggcaatgcg gaaagatcgg ttaaatgttt cggcgcgcca tgttgcgcaa
     2761 taaattccgg cgacgcgcag agaatgcggt aattagtcgc cagtttgcgg gcgattaaat
     2821 taggggcgat gtcatcgcca atgcgaatat cgagatcaac accttcattg accaaatcga
     2881 ccaaccggtc ttccacatca aaacgtaatt ccagttgcgg atacgcctta gccagcgctg
     2941 ataacgccgg agccaccacc tgtcgtccaa aaccaaagct gctgataata cgcagcatcc
     3001 cctgcggcac ctgacgcacg tcagaaagtt cgtccatcat ctgaccgaca tcctgcaaaa
     3061 tccgctgcgc ccattcataa atccgctctc cttcttcggt aatggtgacg cggcgggtgg
     3121 tgcggtgtaa caacaccacg tttagcgttt gctccagcaa ggcgatgcgc ttgctgacga
     3181 acgccggtga aacgcccagt tcttccgcca cggcggcaaa accagcccga cgagccacca
     3241 gcataaagac gcgcaaatca ttcagcagcg gtaaattatt catgattcgt gttttatgtt
     3301 tcaccagtta cggggattaa ttccttttca gtcaattata ggatggtgat gttgtcaatt
     3361 ttgatggtca ggaagtgaga acccaatgat gaaaacgatg cgtattgctg cgatcccggg
     3421 agacgggatt ggcaaagaag tccttcctga agggattcgc gtgttacagg ctgccgctga
     3481 gcgctggggc ttcgccttga gttttgagca aatggagtgg gcgagctgcg agtattacag
     3541 ccatcacggt aaaatgatgc cggacgactg gcatgagcaa cttagccgtt tcgacgccat
     3601 ctattttggt gccgtcggct ggccggacac cgttcctgac catatttcgt tgtggggttc
     3661 gctgctgaaa tttcgtcgtg aattcgacca gtacgtcaac ctgcgcccgg ttcgtctctt
     3721 tcctggcgtt ccctgcccgc tggcgggaaa acagcctggc gacatcgatt tttacgtggt
     3781 cagggaaaac accgaaggcg aatattcctc gctcggcggt agagtgaatg aaggtacaga
     3841 gcatgaagtc gtcattcagg aatcggtatt tacgcgtcgt ggtgtcgatc gcattttgcg
     3901 ttatgccttc gaacttgcgc aaagccgccc acgtaagacg ctaacttctg ccactaaatc
     3961 aaacggttta gccatcagca tgccgtactg ggatgagcga gtggaagcaa tggccgagaa
     4021 ttacccggag atccgctggg acaagcagca tattgatatt ctctgcgcgc gttttgtgat
     4081 gcagccggaa cgcttcgatg tggtggtggc gtccaattta tttggcgata tcctttccga
     4141 tcttggcccg gcctgcaccg gcaccattgg cattgcccca tccgccaacc tgaatccgga
     4201 acgcactttc ccgtcgctct tcgagccagt ccacggttcc gcgccggata tctacgggaa
     4261 aaacattgct aaccctatcg ccacgatttg ggccggtgca atgatgctcg attttctcgg
     4321 caatggcgat gagcgtttcc agcaagcgca taacggtatt ctggcagcaa ttgaagaagt
     4381 gattgctcac gggccgaaaa cacctgatat gaaaggcaat gccaccacgc cacaggttgc
     4441 cgacgcgatt tgcaaaatta ttttgcgtta aggtcaaacc agtttatttg aaccgcgtca
     4501 ctgacgcggt ttttttattc gttctttgca gtaaataacc tgcgtcattt caccttttat
     4561 tgtttccgtt tcgtgtttta tggctttccg tattcttaat tgtttaattt atgtaacatg
     4621 caaataatca atttgttaaa cacaacatac aactcattac cctgcaaata attacaactg
     4681 gcaaaagcca gagacgggct tttatgccca ggagatgact catgatgagc aatgttaaga
     4741 aaaaagatgt gccgctgata agcatcagcc tggtggccat tcttttcatc gcagctgcat
     4801 taagcctttt cccacaacaa tcggccgacg cggccaacgc aatatacact tttgttacgc
     4861 gtacgttagg ttccgccgta caggtattgg ttttgctggc aatgggactg gtgatttatt
     4921 tagccaccag taaatacggc aatattcgtc ttggcgaagg aaaaccggaa tacagcacgc
     4981 tctcctggct gtttatgttt atttgtgccg gtttaggttc ttctacgctt tattgggggg
     5041 ttgctgaatg ggcctattat tatcaaacgc ctggattaaa tatcgcaccg cgttcacaac
     5101 aggcactcga atttagcgtc ccttactcct tcttccactg gggcatcagc gcctgggcaa
     5161 cttatacgct ggcctcatta atcatggctt atcactttca tgtgcgtaaa aacaaaggtc
     5221 tgagtctttc cggcattatt gccgcgatta ccggcgttcg cccgcaaggc ccatggggaa
     5281 aactggttga tttgatgttc ctgatcgcca ctgtcggcgc actgactatt tcccttgttg
     5341 tcaccgcagc aacctttact cgtggacttt ccgcgctgac cggtttaccc gataacttca
     5401 ccgtgcaggc atttgtgatc ctgctttccg gcggcatttt ttgcctaagc tcgtggattg
     5461 gtatcaacaa cggtttgcaa cgtctgagca aaatggttgg ctggggcgcg ttcctgctgc
     5521 cattactggt gctgattgtc ggcccaaccg aatttattac caacagcatc atcaatgcca
     5581 tcggtctgac cacgcaaaac ttcctgcaaa tgagcttatt caccgatccg cttggcgatg
     5641 gttcatttac ccgcaactgg accgttttct actggctgtg gtggatctca tacacccctg
     5701 gcgtagcaat gtttgtcacc cgcgtttccc gcggtcgtaa gattaaagaa gttatctggg
     5761 gactgatcct cggcagcacc gtcggttgct ggttcttctt cggcgtaatg gaaagctatg
     5821 ccattcatca gtttatcaat ggcgtaatca acgtcccaca ggtgctggaa acactgggcg
     5881 gcgaaacagc tgtacagcaa gttctgatgt cgttgccagc cggtaaattg ttcctcgccg
     5941 catacctggg cgtgatgatt attttccttg cctcgcatat ggatgcggtg gcctacacca
     6001 tggctgcgac cagtacgcgt aatctccagg aaggtgacga tcctgaccgt gggctgcgtc
     6061 ttttctggtg cgtggtgatc actctgatcc cgctttccat cttgtttacc ggtgcttcgc
     6121 tggaaacgat gaaaaccacc gtcgtgctca cagcccttcc cttcctcgtc attttactgg
     6181 tgaaagtcgg cggatttatt cgctggctga aacaggatta cgccgacatt ccggctcatc
     6241 aagttgaaca ttatctcccg cagacaccgg ttgaagccct ggaaaaaaca ccagtgctcc
     6301 ctgcgggaac cgtattcaaa ggcgacaact gagcgccatc atcctaacga taaaggtatc
     6361 cctatgagca atctgagccc tgactttgta ctacccgaaa atttttgcgc taacccgcaa
     6421 gaggcgtgga ccattcctgc ccgtttttat accgatcaga acgcgtttga acacgaaaaa
     6481 gagaacgtct tcgccaaaag ctggatttgc gtcgctcaca gcagcgaact ggcgaatgcc
     6541 aatgattatg tgacgcgtga gatcattggc gaaagcatcg tgctggtacg cggtcgtgat
     6601 aaggttttgc gcgcgttcta taacgtgtgt ccgcaccgtg gtcatcagtt gttgagcggt
     6661 ggaggaaaag caaaaaatgt gattacctgc ccgtatcacg catgggcatt caaactcgat
     6721 ggcaatctgg cccatgcacg taactgcgaa aacgtcgcca atttcgatag cgacaaagcg
     6781 caactggttc cggtgcgtct ggaagaatat gccggattcg tcttcatcaa catggacccc
     6841 aacgccacca gcgtagaaga tcagttaccc ggtctgggcg cgaaagtgct ggaagcctgc
     6901 ccggaagtcc acgatctgaa actggcggcc cgctttacca cccgcacgcc tgccaactgg
     6961 aagaacattg tcgataacta tctcgagtgc tatcactgtg gtccggcgca tccaggtttc
     7021 tccgactccg tacaggttga tcgttactgg cacaccatgc acggtaactg gacgctgcaa
     7081 tacggtttcg ccaaaccgtc cgaacagtcg tttaaatttg aagagggtac ggatgcggca
     7141 ttccacggtt tctggctgtg gccgtgcacg atgctgaacg tcaccccgat caaagggatg
     7201 atgacggtca tttatgaatt cccggtggat tctgaaacta ccctgcaaaa ctacgatatt
     7261 tacttcacca atgaagagtt aaccgacgag caaaaatcgc tgattgagtg gtatcgcgat
     7321 gtgttccgtc cggaagattt acgtctggtt gaaagcgtac agaaagggct gaaatcgcgt
     7381 ggctatcgtg gtcaggggcg catcatggcc gacagtagcg gtagtggcat ttccgaacat
     7441 ggtatcgccc atttccataa tctgctggcg caggtgttta aggactaatg acatcggcgg
     7501 cggtattttc cgccgctggg ctgatttttg atggagtaca gcaatgtcag actatcaaat
     7561 gtttgaagta caggtgagtc aggtggaacc gcttaccgaa caggtgaaac gcttcacgct
     7621 ggtggcaacc gatggcaaac cgctgcctgc gttcaccgga ggaagtcaca tcattgtgca
     7681 gatgagcgat ggtgataacc attacagcaa tgcgtattca ctactgagtt cgccgcatga
     7741 cacctcttgt tatcagattg ccgttcggct ggaggaaaac tcgcgcggcg gttcccgctt
     7801 tttgcatcag caggtaaaag tgggcgatcg gttaacgatt tcaacgccta ataacctgtt
     7861 tgcgctaatt ccctcagcca gaaagcatct gtttatcgcg ggcggtattg gtatcacccc
     7921 tttcctgtcg cacatggcag agctgcaaca cagcgacgtc gactggcagc tacattactg
     7981 ctcgcgaaat ccagaaagtt gcgcatttcg tgatgagcta gtccagcatc cgcaggctga
     8041 gaaagttcac ttgcatcact catcaaccgg aacacgactg gaattagcgc gattattggc
     8101 ggatatcgaa cctggcacac acgtttatac ctgtggtccc gaggcgctaa atgaagcggt
     8161 aagaagtgaa gctgcgcgtc tggacatcgt cgccgatacg ctgcactttg agcaatttgc
     8221 tatcgaagac aaaaccggcg atgcctttac cctggtgctt gcccgttccg gaaaagagtt
     8281 tgtggtgccg gaagagatga ctattttgca ggttattgaa aataataaag ccgcgaaagt
     8341 ggaatgttta tgtcgtgaag gggtatgcgg aacctgcgaa acagcaatac tggaaggtga
     8401 agctgaccat cgggatcagt attttagcga tgaagagcgt gccagccagc aaagtatgtt
     8461 gatctgttgt tcgcgtgcga agggtaaacg cctggtgttg gatttgtagt ttgccaaagc
     8521 cggatgtggc gctgagcgcg cccagtccgg cttcggaaga ttttactgcg gatattcctg
     8581 caataaattg tgtaatgctt ccgccatcag ctcaccacgc cagccggaaa tcagctccgg
     8641 caaattgttc tgcggtttca gtttccagtg ccagttcagc agttggttga tttgccgacg
     8701 cgatgccagc aattcggcgc tgatcttatg cgtttcgctc acgtcagtaa tcagcgactt
     8761 aatcgcttta aacactttac gataaccagg catgtccatc aggttaagca tcggctgcgg
     8821 taaggcctct tccggcaatg tctgcgcttt ttccaccagc gccagcagcg ttttaccgtg
     8881 aaagcggatt tcgctaccgg ataaacccag gctgtccagt tcgcctaaac ttcccggcat
     8941 ataacgcgcg accgaccaca aatgctcttc acgcacgaca aagttcaccg ccagatcacg
     9001 ctctcgcgcc ttgcgcagtc gccagtcggc taacagttgc agacaggcca gttggcgtgt
     9061 gcgtaactgc caggcattgg tgatatcacg ccaggcatct tccggcgcaa cgacttcctg
     9121 acgacgcatt tgcatcaggc ggcattcatc cagcgccgca ggtagccagc cggaggcctc
     9181 cgtttctacc ataagcttgg cggtgatcgg taacagatac cagacatccg ccgctgcgta
     9241 ttcacactga cgttcggtca gcggtctggc cagccagtcg gtgcgcgatt cactcttgtc
     9301 cagcgtaacg ccggaatact cttccaccat ggaagcgaaa ccccatgaca tcgggcgtcc
     9361 gcagaaggca gcaaggattt gcgtgtcaat caagggttgt ggtaattcgc caaagacatt
     9421 gaggaacact tccagatctt cactgcctgc atggagaaat tttgtgatgg acggatcgcg
     9481 caggatcgct ttcagcggtg accagtcggt gatcccgaga ggatcaatta gcgccagatg
     9541 ctcgccatcg aaaagttgaa tcaaccccag ctgcgggtaa taagtgcgcg tacgaacaaa
     9601 ttcagtatcc agggctatcg ccggaaaggc acggacggct tcacacaaag aagccagcgc
     9661 atcgtccgtg gtaatcattt ggtaattcaa attgttttct ctttagtggg cgtcaaaaaa
     9721 aacgccggat taaccggcgt ctgacgactg acttaacact caggctttat tgtccacttt
     9781 gccgcgcgct tcgtcacgta attctcgtcg caaaattttt ccgacgttag atttcggtaa
     9841 ctcatcacga aactccacca gcttcggtac tttgtagccc gtgagctgac ggcggcaaaa
     9901 ggtcaccagt gactcttcgg taagcgatgg atcttttttc actacgaaga ttttcaccgc
     9961 ttcaccactg gagccggaag gtacgccaac agccgcgact tcctgtacgc caggatgctg
    10021 catgacgaca tcttcaatct cgttgggata gacgttaaaa ccggaaacca gaatcatgtc
    10081 ttttttacga tcgacaatgc gcaggaaccc ttcttcatcc atcaccgcga tgtcgccggt
    10141 gtgtaaccag ccatttttga tgatctcatc tgtagcatcc ggacgctgcc agtaacccag
    10201 catcacctgc ggtcctttga cacaaagctc acccggttga cccggtggta cttcattatc
    10261 atcatcatcc accagtttgg cttccgtcga cggcaccggc aaaccgatgc taccactatg
    10321 ataatcaata tcatatgggt taacgctgac cagcggcgca cactcggtaa ggccatagcc
    10381 ttccagcaga tactgtcctg tcagtttcac ccaacgctct gccaccactt gctgcactgg
    10441 catccctccg cctgcggaaa gatgcagact ggagaaatcc agctgctgga actctttatt
    10501 gttcagcaac gcattgaaca aggtgttaac gcccgtgata gcggtaaacg gatatttcgc
    10561 taactctttt accaaccctg gaatatcgcg cgggttagtg ataagcaggt tctgcccacc
    10621 cagttcgata aacagcaggc agttaatggt cagggcaaaa atgtgataca gcggcagcgc
    10681 cgtcaccacc agctctttgc ccggatgcaa cagcggacca taggtcgcgt taacctgttc
    10741 caggttcgcc agcatattgc ggtgagtcag catcgcgcct ttcgccacac cagtggtgcc
    10801 gccggtgtat tgcagaaaag ctaaatcttc cggcaccagt tcgggtttga cgtactgcat
    10861 ccggtagccg ttatgcagtg cgctacgaaa tgaaatggca tctggcagat ggtatttcgg
    10921 caccaaacgc ttgatgtatt taacaacgaa attgactacc gtgccttttg ccgtagatag
    10981 ctgatcgccc atacgggtca gaattacgtg ctgaacggcg gttttatcaa ccactttttc
    11041 cagtgtgtga gcaaagttag acacgataac aatcgccgat gcgccgctat cgttaagctg
    11101 atgctcaagc tcacgcgggg tatacaacgg gttaacgttt acgacgatca tcccggcacg
    11161 caaaatgcca aacagcgcca ccggatattg caataaatta ggcatcatca acgcaacgcg
    11221 atcgcctttc ttcagcccca acccttgttg caaataagcg gcaaacgcgc gactgcgttc
    11281 ttccagcttg cggaaggtca ttacctcccc catattcaca aacgcaggtt gatcggcgta
    11341 gcgcgcgacc gactgctcaa acatatctac cagagattga taacggtcag ggttgatctc
    11401 cgtcggaacg tccgcgggat aacggttaag ccaaaccttc ttcaattctt cacctctaaa
    11461 atgcgtgttc gtcgtcatcg caaccccaaa tgatatacat gccgttaaca taatattaac
    11521 tcatcatacc agcttgataa ttacccaacg aaaaggttgc gaagcgcgtc actatttatt
    11581 tttatcttta ccgtaagaat gcagaaacag cggaccagcc gctgtttctt tttctttaaa
    11641 aacaagcaat tattcagtta caactgtttg tactctcgcg gggccgggat tataccagcc
    11701 ccatccgccg tagccatagg gccagccacg accgccataa aaccatggat caatcggctg
    11761 aggcggcata atcacctgct gggttaaatg ccaacgtttg taacccgtta cttgcatcac
    11821 cataaattta tagggcgtat tgccgatttt gccgtcaacc gcaccagtga ttggcccgac
    11881 taccgtaaca agttgtccac ggaaatccac cgggtccaga aaaccgttca catcggcata
    11941 aatgcgaccg cgagaaggtt ctcccagcgt cggtctggct ccgctgtcca gcggtacggt
    12001 agcaatttcc aggcgggttt tcccttgctg gttttgtacc gcaaccactt tgccaccaaa
    12061 gcgtgcctcc tgaccaacgt acagctgcgg cgcactcatc acccgaacta aatcttgttg
    12121 cggcgtggga ctgctgcctt taatggcgtc cggcacagtg acacaaccgc tcagcattag
    12181 cgcaaacgta cctgccagta tgcctttgat aacattcttt tgaaccgcca tgatgcgact
    12241 cctttttttc tcagggcata ctcttaagat tcattctttg cccggaagtt tcttccatgc
    12301 gacgttgtta cgtaaataaa ccggttcggc atgttccacc gccaccgttt taccctcggc
    12361 aaacatctga cacgcaatcg gcagcatatc ttccgccgct ggcagtaaaa cttctccgtc
    12421 gcgcaaaacc agcccgctct ctttaccgag atccggccag gcttgccagc ccgtccctac
    12481 cgttacccat tcgccggaaa gctgttgcat tcgttcatga acgagttcgg gtttgagtac
    12541 ggcttcggtt tcttcaccgc gccagatacc gttttcatca cgctgatatt cggcccagta
    12601 aacttcgccc attcgcgcgt caatggctgc cagcacgcgg gttgcgccgt ttttgcgcca
    12661 cgccccttgc gccatcgtca ttagcgtgga gacgccaatc atcggtagct ctgcgccaag
    12721 tgccagccct tgcgcaatgc caataccaat gcgcacgccg gtaaagctac cggggccgcg
    12781 cccgtaagcc agagcgttaa tatcagtcag ggaagttccg ctggtggtca ggatatcctg
    12841 caccatcggt aagattcgtt gagtatgttc acgagggcaa agctcaaaat gagcgttgac
    12901 agtaccgtcg ttccacaggg cgacagagca ggcctctgtc gcggtatcga tagccagaat
    12961 tcgcatgggt cttcgtgctt agatcaataa aaaggcgcgc atcataccat actccgtaac
    13021 aaattacctg gaggatggta tcgcaaggaa acgaaccgca cgggcaatgt cacgagtgcg
    13081 tggcgcgggc ggcagactgg cgagaaacgt cgcgccgtaa ggacgcatca ccagccgatt
    13141 gtcacaaatc accagcacgc cacgatcgtc ggcgtcgcga atcagtcgcc ctaccccctg
    13201 tttgagagta atgacggcat ctggtagttg cacttcatcg aacgggtcgc caccgcgcaa
    13261 acgacaatct tccatgcgcg cttttaacag tggatcatcc ggcgaggtaa acggcaactt
    13321 gtcgataatc accaatgaca atgtatcgcc acgcacgtcc accccttccc agaaactact
    13381 ggttgccaca agaagcgcgt taccggcgct gacaaattgc tgcaacagtt gccctttgct
    13441 ggtttccccc tgcaacaaaa cgggaagcgt catggtagcg cggaactgct cggccagatc
    13501 acgcatcatg gcgtgcgagg tacaaagcat aaaacaacga ccgttgttag cttcgatgat
    13561 cggtcgcagc attgccgcta actggcgagc agaacctggc tggttggttt gcggcagatt
    13621 gcgcggcaca cagagtaacg cctggcggct gtaatcaaac gggcttggca atagcaacga
    13681 ctccgcctgt tctatcccaa gccgcgaggt gaaatgatgc agatcgtcgt tcaccgacag
    13741 cgttgctgag gtgaagatcc agctaccagg cttttgcgcc attaactctt tgaatttatc
    13801 cgccacgctg agcggcgtga gagccagagt aaaatggcgc gaagtgcatt cgtaccagta
    13861 gctgtagccc ggctgattga tctcttttag ccgcttcagc cgtgtgcgat acaacgtagc
    13921 gcgctcaaat gccgcatcca gcaaggcgga acgccccagt gacagtttcg ccacgtcata
    13981 acaaagttcc agggtgtcat cgagcagtaa aaatgcccgc tgaatttgcg gattagctaa
    14041 cagctcacgc aggttaccgc gataacctgg ctcaccgagt tgcagacgaa aatcctgcgc
    14101 actctgggca agacgatcag cgcacttttg taactgctgg gtgtctttta attcggtgcg
    14161 gtaggcgatg gtgatgtctt ttgccaggtc gagcagttgt cgactggaga gtgactgacc
    14221 aaaatactgg ctggcaatgt ccggtagctg gtgggcttcg tcgaagatca tgacgtccgc
    14281 ttccgggatc agttcgccaa atccactctc tttaaccacc atatccgcca gaaagagatg
    14341 atggtttacc accaccacat cggcgtccat cgctttttta cgtgctttga ccacaaagca
    14401 atctttatac atcgggcagt cgctgccaag acagttgtcg ttggtgctgg tgaccagcgg
    14461 ccacgcctgt gaatcttccg ccacgctgac gcaggtgctg atatcaccat cgactgtttg
    14521 attagaccaa gagcgcagca ggatcacatc gcttaagatt tgtaccggca gatcgccccc
    14581 cgccagcgcc tgctgttcga gacgttcgag gcagaggtag tttgagcgcc ctttcagcag
    14641 cgccacgttg cccgtatatt tcaatgcctt tgagactgtt ggcaaatcgc ggctgtagag
    14701 ctgatcctgc aacgcttttg agccggtcga gataatgact ttctttttcg cccgcagcgc
    14761 aggagccagg taagcgtagg ttttgcccgt accggttcct gcttccacca ccagcggctg
    14821 gcctttttct atcgcctggg tgacggctac cgccatctgt cgctgtggtt ctcgcggctt
    14881 aaagcctggt atcgctttcg ccagctgacc gtctggtgca aaatcgtccg tcacactacc
    14941 ccctgttgat ttgaacaggg attatgtcag gatgagggcg ctttcgccag ttgaagtggt
    15001 gacggcaacc tcacattgtg gcagtctttg cagcacgaaa atggaagtat aatgaggaca
    15061 aaatgatgac tatcgttcgt atcgatgctg aagcccgctg gtctgatgta gtaatccaca
    15121 acaacacgct ctactacact ggtgtaccgg aaaacctcga cgccgatgct tttgagcaaa
    15181 ccgccaacac gctggcgcag attgacgccg tgctggaaaa acatggcagc aataaatcga
    15241 gcattctgga tgccaccatt ttcctggctg ataaaaacga cttcgcggcg atgaataaag
    15301 cgtgggatgc ctgggttgtg gcgggtcatg cgccggtgcg ctgcacggtg caagcggggc
    15361 tgatgaaccc gaagtataaa gttgaaatta agattgtggc tgcggtgtaa gctttatcga
    15421 agcaaaataa gtcagacgat aatttatcga taatactggt cggttttaca taaatcgacc
    15481 agagattaag atattactgt tgatgctcct gctaattcat tatttatttt tataatttca
    15541 attttagcta taaagctaac tatataaata aacataacca tgccgttgtg ttcgcccttc
    15601 gattagcacc tctggttttc tttcttagct gaacaatctg gttatagcgt gcggactgac
    15661 cgttaatttt ctccatctta ctggataaca caccatgagc tgctaccttt aaaacacctg
    15721 aatgtcaaca ggttaactcg tgccatatcg ttataatccc tttgatatta gatgcaaatt
    15781 aaggtcatat atagccttat cgataacat
//
LOCUS       DAZGSP010000118        15736 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZGSP010000118 DAZGSP010000000
VERSION     DAZGSP010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.119"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            191..433
                     /gene="yjbE"
                     /locus_tag="ACHK74_004203"
     CDS             191..433
                     /gene="yjbE"
                     /locus_tag="ACHK74_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFP0222922.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            547..1185
                     /locus_tag="ACHK74_004204"
     CDS             547..1185
                     /locus_tag="ACHK74_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFP0222923.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1182..1919
                     /locus_tag="ACHK74_004205"
     CDS             1182..1919
                     /locus_tag="ACHK74_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP0222924.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1919..4015
                     /locus_tag="ACHK74_004206"
     CDS             1919..4015
                     /locus_tag="ACHK74_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP0222925.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4062..4340)
                     /locus_tag="ACHK74_004207"
     CDS             complement(4062..4340)
                     /locus_tag="ACHK74_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222926.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4356..4484
                     /locus_tag="ACHK74_004208"
     CDS             4356..4484
                     /locus_tag="ACHK74_004208"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222927.1"
                     /translation="MVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYPAYG"
     gene            4610..5020
                     /gene="psiE"
                     /locus_tag="ACHK74_004209"
     CDS             4610..5020
                     /gene="psiE"
                     /locus_tag="ACHK74_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFP0222928.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5064..6539)
                     /gene="xylE"
                     /locus_tag="ACHK74_004210"
     CDS             complement(5064..6539)
                     /gene="xylE"
                     /locus_tag="ACHK74_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFP0222929.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6911..7801)
                     /gene="malG"
                     /locus_tag="ACHK74_004211"
     CDS             complement(6911..7801)
                     /gene="malG"
                     /locus_tag="ACHK74_004211"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFP0222930.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7816..9360)
                     /gene="malF"
                     /locus_tag="ACHK74_004212"
     CDS             complement(7816..9360)
                     /gene="malF"
                     /locus_tag="ACHK74_004212"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFP0222931.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9514..10704)
                     /gene="malE"
                     /locus_tag="ACHK74_004213"
     CDS             complement(9514..10704)
                     /gene="malE"
                     /locus_tag="ACHK74_004213"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFP0222932.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11069..12184
                     /gene="malK"
                     /locus_tag="ACHK74_004214"
     CDS             11069..12184
                     /gene="malK"
                     /locus_tag="ACHK74_004214"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFP0222933.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12256..13596
                     /gene="lamB"
                     /locus_tag="ACHK74_004215"
     CDS             12256..13596
                     /gene="lamB"
                     /locus_tag="ACHK74_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFP0222934.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13839..14759
                     /gene="malM"
                     /locus_tag="ACHK74_004216"
     CDS             13839..14759
                     /gene="malM"
                     /locus_tag="ACHK74_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFP0222935.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14988..15736
                     /locus_tag="ACHK74_004217"
                     /pseudo
     CDS             14988..15736
                     /locus_tag="ACHK74_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579094.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT         3949 a         3964 c         4074 g         3749 t
ORIGIN      
        1 attttaacat tatgattttt aatgttattt atatataagt tgtagcttat atttgatatt
       61 cattccaatt atcctaaaac cccatctcta attccccgcg ccgtaattcg catgctttag
      121 ttgtgtatac tcgatcccgc ccgaaatgtt tttgggtaaa tctccattca ttcaatgaag
      181 ggaaattgtt atgaaaaaag ttctgtatgg catttttgcc atatctgcgc ttgcggcgac
      241 ttctgcgtgg gctgcacctg tacaggtggg cgaagcggca gggtcggcag caacgtcggt
      301 ttcggcgggg agttcctccg cgaccagcgt cagcaccgta agctcggcgg tgggtgtcgc
      361 gctcgcggca accggtggcg gtgatggttc taataccggg accaccacaa ccacgaccac
      421 cagtacccag taataaagta tgtatcccca aaataatttg agtcattgca tctgtggctt
      481 gaagtatgaa gggatttacc ataaccacac tccggtgtgg ttattctgcc cctctggaga
      541 agagtcgtga agcgacctgc actcattctt atctgcctgc tattacaggc ctgttcagcc
      601 acgactaaag agctgggcaa ttcactgtgg gacagtctgt tcggcacgcc aggcgtacag
      661 ctgacggacg atgatattca aaatatgccc tacgccagcc agtacatgca gcttaatggc
      721 gggccgcaat tgtttgtggt gctggccttc gctgaagacg gacaacaaaa atgggtcact
      781 caggatcaag ccactcttgt cacacaacat ggtcgtctgg tgaagacttt gcttggcggc
      841 gacaacctga ttgaagtgaa taaccttgcc gccgacccgc tgattaaacc cgcacaaatt
      901 gttgatggtg caacatggac gcgcacgatg ggctggaccg agtaccagca ggtacgctac
      961 gccaccgcac gctcagtctt caaatgggat ggcaccgata ccgtcaaggt cggcagcgat
     1021 gaaaccccgg ttcgcgtgct ggacgaagaa gtctccaccg accaggcgcg ctggcataac
     1081 cgctactgga tcgacagcga agggcaaatt cgccagtcgg aacagtatct cggcgcggat
     1141 tatttcccgg tgaaaaccac tctcatcaag gcggcaaaac aatgattaaa caaactattg
     1201 tcgcattgat tttgagtgtg ggggcgtcat cggtcttcgc ggcgggaacc gtcaaggtat
     1261 tcagcaatgg cagcagtgag gccaaaacgc tgacgggcgc agagtattta atcgatctgg
     1321 taggccagcc gcggctggca aacagttggt ggcctggtgc ggtgattagc gaagagctgg
     1381 caacggcggc agcattgcgg cagcagcagg cgttgctgac acggctggca gaactggctg
     1441 cagattccag cgccgacgat gccgccgcga ttaacgcctt acgccagcaa attcaggcgt
     1501 tgaaagtgac gggcaggcaa aaaatcaatc ttgatcctga tatcgtccgc gttgccgaac
     1561 gcggtaaccc accgttgcag ggcaactaca cgctgtgggt cgggccacca ccgtccacgg
     1621 tcacgctgtt cgggcttatc agccgtcctg gcaagcagcc attcactcca ggtcgcgacg
     1681 tggcgagcta tctctctgac cagagcctgc tcagcggtgc ggatcgcagc tacgcgtggg
     1741 tcgtttaccc ggacggacgc acacaaaaag cgccggtggc ttactggaac aagcgtcacg
     1801 tagagccgat gcccggcagc attatttatg ttggcctcgc ggactccgtc tggagtgaga
     1861 cgcctgatgc ccttaacgcc gacattcttc agactctgac gcagcggata ccccaataat
     1921 gaaaaaaaga catctgctta gcttactggc gctgggcatt agcacagctt gctacggcga
     1981 aacatatcct gcgcccattg gtccgtcgca gtcggatttc ggtggcgtag gattattaca
     2041 aacgcccacc gcgcgcatgg cgcgggaagg ggagttgagc ctgaactatc gagataacga
     2101 tcagtaccgt tattactcag cttcagtgca actcttcccg tggctggaaa caacgctgcg
     2161 ctacaccgac gtgcgcaccc ggcagtacag cagcgtcgaa gcgttctctg gcgatcaaac
     2221 gtataaagat aaagccttcg atctcaaact gcgtttgtgg gaagagagtt actggctgcc
     2281 gcaagtggcg gttggtgcgc gggatatcgg cggtacgggg ctgtttgatg cggaatatct
     2341 tgttgccagc aaagcctggg ggccgttcga ttttacgctc ggcctgggct ggggatatct
     2401 gggcaccagc ggtaatgtga aaaatccgct ctgttcagcc agtgataaat attgctatcg
     2461 cgataacagc tacaaacagg cgggatctat cgacggtagc cagatgttcc acggtcctgc
     2521 ctcactgttt ggcggcgtgg aataccagac gccctggcaa ccgctgcgcc tgaaactgga
     2581 gtatgaaggc aataattatc agcaggattt tgccgggaag ctggagcaaa aaagtaagtt
     2641 taacgtcggt gcgatttatc gcgttaccga ttgggccgac gttaacctta gctatgaacg
     2701 tggcaacacc tttatgtttg gcgtcacgct gcgcaccaac tttaacgatc tgcgcccgtc
     2761 ctacatcgat aacgcccgcc cgcaatatca accgcagccg caggatgcca ttttgcagca
     2821 ttcggtggtg gcgaatcagt taacgctgtt gaaatacaat gccggacttg ccgatccgca
     2881 gatccaggcg aaaggcgata cgctgtatgt taccggcgag caggtgaaat atcgtgattc
     2941 gcgcgaaggg atcatccgtg ctaatcggat cgtgatgaat gatctgccgg atgggatcaa
     3001 aacgatccgc gttacggaaa atcgccttaa catgccgcag gtgacgacgg aaaccgatgt
     3061 cgccagcctg aaaaatcatc tcgccggaga gccgttgggc cacgaaacga cgctggcgca
     3121 aaaacgcgtc gagccagtgg ttccgcagtc caccgagcag ggctggtata tcgacaaatc
     3181 acgctttgat ttccatatcg atccggtgct gaaccagtcg gtcggtggcc cggaaaactt
     3241 ttacatgtat cagctgggcg tgatgggaac ggcagatttg tggctgacgg accatctact
     3301 gaccaccggc agcctgtttg caaatcttgc caacaactac gacaagttta actacaccaa
     3361 tccaccgcag gactcgcact taccgcgcgt tcgtacccat gtgcgcgagt atgtgcagaa
     3421 cgatgtctat gtgaataacc tgcaagccaa ctacttccag catctgggca acggcttcta
     3481 cggtcaggtc tacggtggtt atctcgaaac catgtttggc ggtgcggggg cagaagtgtt
     3541 gtatcgcccg ctggacagca actgggcgtt tggtctggat gccaactacg ttaaacagcg
     3601 cgactggcgt agtgcaaaag atatgatgaa attcaccgac tacagcgtga aaaccggaca
     3661 tctgaccgcc tactggacgc catctttcgc tcaggatgtg ttagttaaag ccagcgtcgg
     3721 gcagtatctg gcaggggata aaggcggcac gctggagatc gccaaacgct ttgatagcgg
     3781 cgtggtggtg ggtggctatg ccacgatcac taatgtttca aaagaggagt acggcgaagg
     3841 ggacttcacc aaaggcgtgt atgtctcggt accgttggat ctcttctcgt ctggcccgac
     3901 acgcagccgt gcggcgattg gctggacgcc gctgacgcgt gacggtggtc agcaacttgg
     3961 gcgtaagttc cagttgtatg acatgaccag cgaccgtagc gtcaatttcc gctaagtcat
     4021 gggaaaggtg ccagttttcg caatcactgg caccctcatt cttaatggcc tgttgagcca
     4081 tcaggaacgc agttcacccc cggtttgcga tctggtgtgt aaaggcgaaa acattctgtc
     4141 ttcctgtccg tcgatatcgg ttgcagatct ggttgcctga gcgactgggc catcattggg
     4201 ctggagacat tgctttgcgg taggtcggct ttatcagcag taccgagcgg accagcataa
     4261 gcaggaagaa cagagactga taacatcaaa gcagcaaaat aaggcttcat ttttaccacc
     4321 tttatcaggt tacgtttcat ttgttccaga ggaacatggt cgatttttcg cgcattgctg
     4381 gtggctggga atcacctgaa tgggtgattt ttgaattacc ggctttggtg cggtttgtct
     4441 tgccggatgc gccgccaggc gcggcttatc cggcctacgg gtaggtatat ccggctttgg
     4501 tggaggcgcg ctccaaatcc aggttgaaca aaacatacac aaaaaatata gatctccgtc
     4561 acatttttgc gttatacagg aagctcgcca ctgtgaagga ggtactgcta tgacgtcact
     4621 ctctcgtccg cgcgtggagt ttatctccac cattttgcag accgtactca atcttggcct
     4681 gttgtgcctc ggcctgattt tggttgtctt cctcggcaaa gaaacggtgc atctggcaga
     4741 tgtgctgttc gcgccagaac aaaccagcaa atatgagctg gtagaaggac tggtggttta
     4801 ctttctctat ttcgaattta tcgcgctgat tgtgaaatac tttcagtccg gttttcactt
     4861 cccgctacgt tactttgtct atattgggat caccgccatt gtgcgcttga tcatcgtcga
     4921 tcataaatcg ccactggatg tgttgatcta ctcggctgcg atcctgttac tggtgatcac
     4981 tctgtggttg tgtaattcga aacggctgaa gcgggagtaa aaagacagca cgccgaactg
     5041 gcgcggcgtg ctggacagga agattacagc gtagcagttt gttgtgtttt cttcgtttcc
     5101 ggttcccaga gggcttccag ctcctcaagg gttttacctt tggtttccgg gacaaatttc
     5161 cacataaata gtgctgccag aacgcccata cacccgtaaa tccagtagga gaaaccgttg
     5221 tggaaatggg ccaccagcca ggagtttttg tccatcatcg ggaaggtcca ggagacgaag
     5281 tagttcgcca gccactgggc cgccaccgcg attgccagcg ctttaccacg aatggcattc
     5341 gggaagattt ccgacagcag aacccagcat accggacccc aggacatggc aaaggcggca
     5401 acatagaaca gcatcgacag tagcgccaca atacccggtg cctgagtgta aaacgcggta
     5461 ccgaggctaa acataccgat tgccattccg agcgcgccga taatttgcag tggcttacga
     5521 ccaaatttat ccaccgtcat aattgccaga acggtgaagg tgaggttgat aactccgaca
     5581 ataatggtct gcaacagcgc gatatccgtg ctggccccca gcgttttgaa cacttccggc
     5641 gcgtagtaca acaccacatt gatgccgaca aattgctgga agatggagag cataacgccg
     5701 atgacaatca cgcccacgcc aaacatcagc agacgaccac cggttttgcg gccatgatcc
     5761 agggagtgtt taatttcctg tactgcctga gttgcaagcg tgttgcccat aattttgcgc
     5821 aggatacttt ccgcctgttc ttgcttgccg cgtgacatca gccagcgagg actttctggc
     5881 acggtataca gcagcattaa gaacagcagt gcagggatac attccgaggc aaacatataa
     5941 cgccagccgt cagtattcag ccagctggca tcaccggaac gggcaataaa atagtttacg
     6001 cagtaaacta aaagttgccc gaaaataatc gcaaactggt taaatgaaac cagttttccg
     6061 cgaatatgag ctggagccag ttccgcaata tacattggtg aaagcattga ggctaaacca
     6121 acgccaatac cgccaataat acgataaata acaaattccg ggacataacc tgccagataa
     6181 ataggtactg tgttgtccgg gtttatagag gtaaaaccaa gttctggcca ggcagaacct
     6241 acaccagaaa taaaaaacag gacagcagca atcttaagtg aatcacgacg gccgaagcgg
     6301 ttactgcaat aaccaccgag ggcaccgccg atgatgcaac caatcagagc gctggctacg
     6361 caaaatccta acagggagtt ggcagcggat tcacttaagt tttgtggagc aacaaagacg
     6421 gtattgagtg actcaacagt accggaaata acggcggtgt cgtagccaaa taataaacca
     6481 cctaatgtag cgactaaggt aatcgaaaat atataactgg aattatactg ggtattcatt
     6541 cagacctgcc ttagaccatt ctgatctttt atcagtgcat tgatgttgga attgtcaggc
     6601 agttaaggca attatcattt tttacaatgc gtttacgtaa tgtcttttct gtgatcttaa
     6661 ttgtgataat tatcaaaaat tgatgaaaac aatactattg ccgtgactca gagcacgaaa
     6721 gagaattatc gtaagtggga aaacaaataa cgtaaaaata ataagttcta tgatgggaat
     6781 attaaccggc gaacgattca gattgcagac gaaagaaaaa aaggcgctcc gtggagcgcc
     6841 gaataacagt cacaagttgg gataacgtaa gttgagggtg cagcggcata acattggcag
     6901 aacaacatct ttaacctttc acaccacctg ccgtcaggcc gttgaccagc cagcgttgag
     6961 ccagcaagaa gacgatggtg atcggtaatg cagacatcac ggcagcggcg gcaaagtcac
     7021 cccacaggta gttttgcggg ttgaggtatt gctgcatccc cacggccagg gtgtagctgt
     7081 ttacgtcacg cagtaacagc gacgcgaccg gaacttcagt aatggcagcg ataaacgaca
     7141 ggatgaacac caccgccaga atcggtactg acagcggcaa caggacaagg cggaaggcct
     7201 gccacggtgt cgcaccatcc agcgccgccg cttcttccag cgaactgtcg atggtttcga
     7261 aatagccttt gatggtccag acatgcagcg caatcccacc cagatacgcg aaaattacgc
     7321 cgccgtgagt attcaggcca atgaatggaa tgtactcacc cagacgatca aacaacgcat
     7381 acaacgcgac cagtgaaagt actgccggga acatctggaa aatcagcatt cctttcagca
     7441 gcgtcgcttt gcctggaaag cgcatacggg cgaaagcgta ggcgcaggtg gtagagagcg
     7501 ccacaatgcc aatcgcggaa atcccagcga cctttaccga gttccatagc cacagcagta
     7561 ccgggaatgg cggtggcgta atgcgaccat cagcctgttc cacgctaaaa cctaacgcca
     7621 gtttccagtg atcccaggag atttgctccg ggatcaggct gccggtcgca aagttcccct
     7681 ggcgcagtga gatagcgacg accatcagca gcgggaacat aatcgctgcg ataaaaagta
     7741 gcagtagcag gtgagtaata aacaaacgtg ctttttgcga tttcggttgg accattgcca
     7801 tttttgttat ctcccttaat caaacttcat tcgcgtggct ttcaggttca ctatcgccag
     7861 cgcacccacc agcaggaaga tcagcgtggc aattgctgcc gccagaccga agtcctgacc
     7921 cccgccgcct tcaaaagcga tgcggtaggt gtagttaaca agcaggtcgg tataaccggc
     7981 tggcgtggtc gtgccaagac gatccgggcc gccgttggtt aacagttgaa tcagcacgaa
     8041 gttgttaaag ttaaaggcga agctggcgat catcagcggc gtcagcggtt taatcagcag
     8101 cggcagcgta atcttaaaga agttctggaa cggacctgca ccatccattg ctgaggcttc
     8161 atacaaatcg tccggaatcg ctttcagcaa gcccatgcag aggatcatca tgtacggata
     8221 acccagccag gtattgacga taatcagcat cgtgcgggcg gtggtcggat cgctgaacca
     8281 ggcgggcttc acgccaaaca gcgcgctcaa catcatgttg atttcaccga agctctggtt
     8341 aaacaaccct ttgaaaatca agattgaaat gaatgatggc accgcgtagg gcagaatcag
     8401 caggacgcga tataccgctt tgccacgcaa cgcttcccac tgcaccagac acgccagaac
     8461 catgccgacc gccaccgtta aaaagacggt gatcagtgaa aacaccacgg tccagacgaa
     8521 aatggcgagg aacggtttct gaatgccttc gtcggtgaag acgcgggtaa agtttttcca
     8581 gccggtggtc acggtgtaac cggggcttag cttttcatcc ccccagttgc cgtcggcggt
     8641 aatggactgg taaaagccaa tttggttatt cggacgatat ttcacgccgc tctgattatt
     8701 cgtcaacgtg ccgtcaccgt cgagtgtgta gagcggctgc gtgccagaaa actggcgcag
     8761 ggagctcatc atcactttgt tgccatccgg cagaatggcg gtaatgtcac tcagcgcctg
     8821 acggttctgg gtaatcacgc gcagattcgc gcgttcgcct tcgggctggg cggtcgtttc
     8881 tttcagttgc agtttttgct cgccgccaaa tttaaacgcg tcggagaggt aatttttgcc
     8941 ggtttcgccg tcgctgagcg ccagttgcca ctcatcgccc gccgggtaaa gaccaaagtt
     9001 ataggttttg cctgcttgcc aggagcgatc taacaacact tcctgcgcac gttcaaaagt
     9061 cagctggtta gtgctgctgt agttggtgaa ggcaatggcg atggtgcaga ccagagggaa
     9121 gaggacgaat aatcccattc cagccattcc cgggtaaaca tagcgccagg cgtaggcttt
     9181 acgattggcg aaaatataca gccccgctga actcaatatc agcgtggtaa tggcgaacag
     9241 gtattcccct tgtgcgtaca ttaaaacaac aaggtaaccc accagcaggc cgagcagacc
     9301 tagcactgac catttcagcg cgtcgctttg ccaccaatgt ttctttttaa tgacatccat
     9361 ggggttcttc ctcattccag gacggataag gctttcacgc cttatccgac aacaactgcc
     9421 tgatgcgaca ctgatgcgtc ttatcaggcc tacatacgtt tcaatttcgt aggccggaca
     9481 aggcgttcac gccgcatccg gcatttcaca gcattacttg gtgatacgag tctgcgcgtc
     9541 tttcagggct tcatcgacag tctgacgacc gctggcggcg ttgatcaccg cagtacgcac
     9601 ggcataccag aaagcggaca tctgcgggat gttcggcatg atttcacctt tctgggcgtt
     9661 ttccattgtg gcggcaatac gtggatcttt cgccaactct tcctcgtaag acttcagcgc
     9721 tacggcaccc agcggtttgt ctttattgac cgcttccaga ccttcgtcag tcagcagata
     9781 gttttcgagg aactctttcg ccagctcttt gttcggactg gcggcgttaa tacctgcgct
     9841 cagcacgcca acgaacggtt tagatggttg acccttgaag gtcggcagta ccgttacacc
     9901 ataattcact ttgctggtgt cgatgttgga ccatgcccac gggccgttga tggtcatcgc
     9961 tgtttcgcct ttattaaagg cagcttctgc gatggagtaa tcggtgtctg cattcatgtg
    10021 tttgttttta atcaggtcaa ccaggaaggt cagacccgct ttcgcgccag cgttatccac
    10081 gcccacgtct ttaatgtcgt acttgccgtt ttcatacttg aacgcataac ccccgtcagc
    10141 agcaatcagc ggccaggtga agtacggttc ttgcaggttg aacatcagcg cgctcttacc
    10201 tttcgctttc agttctttat ccagcgccgg gatctcttcc caggtttttg gtgggttcgg
    10261 tagcagatct ttgttataaa tcagcgataa cgcttcaaca gcgatcgggt aagcaatcag
    10321 cttgccgttg taacgtacgg catcccaggt aaacggatac agcttgtcct ggaacgcttt
    10381 gtccggggtg atttcagcca acaggccaga ttgagcgtag ccaccaaagc ggtcgtgcgc
    10441 ccagaagata atgtcagggc catcgccagt tgccgcaacc tgtgggaatt tctcttccag
    10501 tttatccgga tgctcaacgg tgactttaat tccggtatct ttctcgaatt tcttaccgac
    10561 ttcagcgaga ccgttatagc ctttatcgcc gttaatccag attaccagtt taccttcttc
    10621 gattttggcg agagccgagg cggaaaacat catcgtcgtt aatgcggata atgcgaggat
    10681 gcgtgcacct gtttttattt tcataatcta tggtccttgt tggtgaagtg ctcgtgaaaa
    10741 cacctaaacg gactctagtt tctttatacg gcaacctctt tccatcctcc ttgcccctac
    10801 gccccaccgt tgctttgtgt gatctctgtt acagaattgg cggtaatgtg gagatgcgca
    10861 cataaaatcg ccacgatttt tgcaagcaac atcacgaaat tccttacatg acctcggttt
    10921 agttcacaga agccgtgttc tcatcctccc gcctcctccc ccataaaaaa gccagggggt
    10981 ggaggattta agccatctcc tgatgacgca tagtcagccc atcatgaatg ttgctgtcga
    11041 tgacaggttg ttacaaaggg agaagggcat ggcgagcgta cagctgcaaa atgtaacgaa
    11101 agcctggggc gaggtcgtgg tatcgaaaga tatcaatctc gatatccatg aaggtgaatt
    11161 cgtggtgttt gtcggaccgt ctggctgcgg taaatcgact ttactgcgca tgattgccgg
    11221 gcttgagacg atcaccagcg gcgacctgtt catcggtgag aaacggatga atgacactcc
    11281 gccagcagaa cgcggcgttg gtatggtgtt tcagtcttac gcgctctatc cccacctgtc
    11341 agtagcagaa aacatgtcat ttggcctgaa actggctggc gcaaaaaaag aggtgattaa
    11401 ccaacgcgtt aaccaggtgg cggaagtgct gcaactggcg catttgctgg atcgcaaacc
    11461 gaaagcgctc tctggcggtc agcgtcagcg tgtggcgatt ggccgtacgc tggtggccga
    11521 gccaagcgta tttttgctcg atgaaccgct ctccaacctc gatgctgcac tgcgtgtgca
    11581 aatgcgtatc gaaatctccc gtctgcataa acgcctgggc cgcacaatga tttacgtcac
    11641 ccacgatcag gtcgaagcga tgacgctggc cgacaaaatc gtggtgctgg acgccggtcg
    11701 cgtggcgcag gttgggaaac cgctggagct gtaccactat ccggcagacc gttttgtcgc
    11761 cggatttatc ggttcgccaa agatgaactt cctgccggta aaagtgaccg ccaccgcaat
    11821 cgatcaagtg caggtggagc tgccgatgcc aaatcgtcag caagtctggc tgccagttga
    11881 aagccgtgat gtccaggttg gagccaatat gtcgctgggt attcgcccgg aacatctact
    11941 gccgagtgat atcgctgacg tcatccttga gggtgaagtt caggtcgtcg agcaactcgg
    12001 caacgaaacc caaatccata tccagatccc ttccattcgt caaaacctgg tgtaccgcca
    12061 gaacgacgtg gtgttggtag aagaaggtgc cacattcgct atcggcctgc cgccagagcg
    12121 ttgccatctg ttccgtgagg atggcactgc atgtcgtcga ctgcataagg agccgggcgt
    12181 ttaagcaccc cacaaaacac acaaagcctg tcacaggtga tgtgaaaaaa gaaaagcaat
    12241 gactcaggag atagaatgat gattactctg cgcaaacttc ctctggcggt tgccgtcgca
    12301 gcgggcgtaa tgtctgctca ggcaatggct gttgatttcc acggctatgc acgttccggt
    12361 attggctgga caggtagcgg cggtgaacaa cagtgtttcc agactaccgg tgctcaaagt
    12421 aaataccgtc ttggcaacga atgtgaaact tatgctgaat taaaattggg tcaggaagtg
    12481 tggaaagagg gcgataagag cttctatttc gacactaacg tggcctattc cgtcgcgcaa
    12541 cagaatgact gggaagctac cgatccggcc ttccgtgaag caaacgtgca gggtaaaaac
    12601 ctgatcgaat ggctgccagg ttccaccatc tgggcaggta agcgcttcta ccaacgtcat
    12661 gacgttcata tgatcgactt ctactactgg gatatttctg gtcctggtgc cggtctggaa
    12721 aacatcgatg ttggcttcgg taaactctct ctggcagcaa cccgctcctc tgaagctggt
    12781 ggttcttcct cttttgccag caacaatatt tatgactata ccaacgaaac cgcgaacgac
    12841 gttttcgatg tgcgtttagc gcagatggaa atcaacccgg gcggcacatt agaactgggt
    12901 gtcgactacg gtcgtgccaa cctgcgtgat aactatcgtc tggttgatgg cgcatcgaaa
    12961 gacggctggt tattcactgc tgaacatact cagagtgtcc tgaagggctt taacaagttt
    13021 gttgttcagt acgctactga ctcgatgacc tcgcagggta aaggtctgtc gcagggttct
    13081 ggcgtcgcgt ttgataacga aaaatttgcc tacaatatca acaacaacgg tcacatgctg
    13141 cgtatcctcg accacggtgc gatctccatg ggcgacaact gggacatgat gtacgtgggt
    13201 atgtaccagg atatcaactg ggataacgac aacggcacca agtggtggac cgttggtatt
    13261 cgcccgatgt acaagtggac gccaatcatg agcaccgtga tggaaatcgg ctacgacaac
    13321 gtcgaatccc agcgcaccgg cgacaagaac aatcagtaca aaattaccct cgcacaacaa
    13381 tggcaggctg gcgacagcat ctggtcacgc ccggctattc gtgtcttcgc aacctacgcc
    13441 aagtgggatg agaaatgggg ttatgactac aacggcgata gcaaggttaa cccgaactac
    13501 ggcaaagccg ttcctgctga tttcaacggc ggcagcttcg gtcgtggcga cagcgacgag
    13561 tggaccttcg gtgcccagat ggaaatctgg tggtaatagc aaaacctggg ccggataagg
    13621 cgtttacgcc gcattcggca accaacgcct gatgcgacgc ttgcgcgtct tatcaggcct
    13681 acaacggctg tcaaatgtag gccggataag gcgtttacgc cgcatccggc ataaaaacag
    13741 gttgtcatta tctgaaaggg gcgaaagccc ctctgattat cgggtttagc gcgctattgc
    13801 ctggctaccg ctgagctcca gattttgagg tgaaaacaat gaaaatgaat aaaagtctca
    13861 tcgccctctg tttatcagca gggttactgg caagcgcgcc tggaatcagc cttgccgatg
    13921 ttaactacgt accgcaaaac accagcgacg cgccagccat tccatctgct gcgctgcaac
    13981 aactcacctg gacaccggtc gatcaatcta aaacccagac cacccaactg gcgaccggcg
    14041 gccaacaact gaacgttccc ggcatcagtg gtccggttgc tgcgtacagc gtcccggcaa
    14101 acattggcga actgaccctg acgctgacca gcgaagtgaa caaacaaacc agcgtttttg
    14161 cgccgaacgt gctgattctt gatcagaaca tgaccccatc agccttcttc cccagcagtt
    14221 atttcaccta ccaggaacca ggcgtgatga gtgcagatcg gctggaaggc gttatgcgcc
    14281 tgacaccggc gttggggcag caaaaacttt atgttctggt ctttaccacg gaaaaagatc
    14341 tccagcagac gacccaactg ctcgacccgg ctaaagccta tgccaagggc gtcggtaact
    14401 cgatcccgga tatccccgat ccggttgctc gtcataccac cgatggctta ctgaaactga
    14461 aagtgaaaac gaactccagc tccagcgtgt tggtaggacc tttatttggt tcttccgctc
    14521 cagctccggt tacggtaggt aacacggcgg caccagctgt ggctgcaccc gctccggcac
    14581 cggtgaagaa aagcgagccg atgctcaacg acacggaaag ttattttaat accgcgatca
    14641 aaaacgctgt cgcgaaaggt gatgttgata aggcgttaaa actgcttgat gaagctgaac
    14701 gcctgggatc gacatctgcc cgttccacct ttatcagcag tgtaaaaggc aaggggtaat
    14761 tacgccccac agtgctgatt ttgcaacaac tggtgcgtct cctggcgcac ctttttttat
    14821 gcttccttgc tgggatatga gcgatttttt atagtaactc acttcttctt cactaagaat
    14881 atccattatc tcaatgcctt atcagagatt cttttccttt cgccggttat gtctggacat
    14941 tcaggctact tttccaggtt attttatttc tgttatgcag aggttttatg ataagtcata
    15001 tcctaaattc tggcggcaat aactctttga tgaaacatga tgtggtgcaa ggaaataata
    15061 tagtagatct tgatttacta cgtaatttca atggggtgcc aggtttaaac agagataact
    15121 ttatttatat cagcaatatt ttttcaaata taaaaaaacg gaacgaaaaa atcatgcaat
    15181 aaatttgttt cgtgaagtct caatcagtaa tgatactata agtgtaaaat tctacagaaa
    15241 tgaaaaaaat tgaatgcgct tgcaattttc tgatggataa agatgcgcag gggtatatcg
    15301 acctgtctga tttggattta acaagttgtc attttaaagg tgacgttata tcgaaggtgt
    15361 cttttttatc atcaaatcta caacatgtaa cattcgaatg taaagaaatt ggggattgca
    15421 attttactac tgcaatagtt gataatgtca tatttagatg tcgacgttta cacaatgtga
    15481 tttttatcaa agcgagtggt gaatgtgtcg atttcagcaa aaatattctt gatacagttg
    15541 acttctcgca gagtcaactt actcagagta attttcgcga atgtcagatt agaaattcaa
    15601 acttcgataa ttgttatctt tacgcttcgc acttcaccag agcagagttt ctgtctgcca
    15661 aagaaatatc atttattaaa tcgaatatga cagctgttat gtttgatcat gtgcgaatat
    15721 cgacagggaa ttttaa
//
LOCUS       DAZGSP010000119        15009 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZGSP010000119 DAZGSP010000000
VERSION     DAZGSP010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.120"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1335
                     /locus_tag="ACHK74_004218"
     CDS             <1..1335
                     /locus_tag="ACHK74_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0222936.1"
                     /translation="SGTILSDNYNAITLGNGWNTPLGAISFYAARSSSKLNNDTRHEG
                     TSYQVAYNKYLVQTATRFSVAAWRYASQDYRTFSDHLYENDKHYHQSDYDDFYDIGRK
                     NSLSANIMQPLSNNLGNVSLSALWRNYWGRSGNAKDYQFSYSNNWQHISYTFSASQSY
                     DENNKEEERFNLFISIPFYWGDDIAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEH
                     DQLNYGIYVNQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWP
                     GGINITNQLSDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIA
                     NSESETELQGNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQE
                     NNIGVVGQGSRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            1348..2306
                     /locus_tag="ACHK74_004219"
                     /pseudo
     CDS             1348..2306
                     /locus_tag="ACHK74_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(2345..3244)
                     /gene="nhaR"
                     /locus_tag="ACHK74_004220"
     CDS             complement(2345..3244)
                     /gene="nhaR"
                     /locus_tag="ACHK74_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFP0222937.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(3310..4476)
                     /gene="nhaA"
                     /locus_tag="ACHK74_004221"
     CDS             complement(3310..4476)
                     /gene="nhaA"
                     /locus_tag="ACHK74_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFP0222938.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            5062..5214
                     /gene="mokC"
                     /locus_tag="ACHK74_004222"
     CDS             5062..5214
                     /gene="mokC"
                     /locus_tag="ACHK74_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFP0222939.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(5318..6448)
                     /gene="dnaJ"
                     /locus_tag="ACHK74_004223"
     CDS             complement(5318..6448)
                     /gene="dnaJ"
                     /locus_tag="ACHK74_004223"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFP0222940.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(6537..8453)
                     /gene="dnaK"
                     /locus_tag="ACHK74_004224"
     CDS             complement(6537..8453)
                     /gene="dnaK"
                     /locus_tag="ACHK74_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFP0222941.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            8830..9234
                     /gene="yaaI"
                     /locus_tag="ACHK74_004225"
     CDS             8830..9234
                     /gene="yaaI"
                     /locus_tag="ACHK74_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFP0222942.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            9260..9973
                     /gene="msyB"
                     /locus_tag="ACHK74_004226"
     CDS             9260..9973
                     /gene="msyB"
                     /locus_tag="ACHK74_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP0222943.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            10122..10688
                     /gene="satP"
                     /locus_tag="ACHK74_004227"
     CDS             10122..10688
                     /gene="satP"
                     /locus_tag="ACHK74_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFP0222944.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(10723..11310)
                     /gene="mog"
                     /locus_tag="ACHK74_004228"
     CDS             complement(10723..11310)
                     /gene="mog"
                     /locus_tag="ACHK74_004228"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFP0222945.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(11425..12378)
                     /gene="tal"
                     /locus_tag="ACHK74_004229"
     CDS             complement(11425..12378)
                     /gene="tal"
                     /locus_tag="ACHK74_004229"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP0222946.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            12657..14087
                     /gene="yaaJ"
                     /locus_tag="ACHK74_004230"
     CDS             12657..14087
                     /gene="yaaJ"
                     /locus_tag="ACHK74_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFP0222947.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            14157..14933
                     /gene="yaaA"
                     /locus_tag="ACHK74_004231"
     CDS             14157..14933
                     /gene="yaaA"
                     /locus_tag="ACHK74_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFP0222948.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
BASE COUNT         3798 a         3719 c         3560 g         3932 t
ORIGIN      
        1 tcgggtacga ttttatccga taactataat gctataactc tgggaaatgg ttggaataca
       61 ccgctgggtg ccatctcttt ttacgctgcc cgatcgagta gtaagttaaa taatgatacc
      121 agacacgaag gtacgagcta tcaggtcgcc tataataaat atctagtaca aacagccact
      181 cgatttagcg tggccgcctg gcgttatgct tcgcaggatt acaggacatt cagcgaccat
      241 ctttacgaaa atgataaaca ctatcatcag agtgattatg atgattttta tgatattggc
      301 agaaaaaata gcctttctgc taatattatg caacctttat ccaataatct gggaaatgta
      361 tcattaagtg cactttggcg gaattactgg gggcgaagtg gaaatgctaa agattaccaa
      421 ttcagttact ccaataactg gcaacacatt agttatactt tctctgccag ccaatcttat
      481 gatgaaaata ataaagaaga ggagcgtttt aatctgttta tctccattcc tttctactgg
      541 ggggatgata ttgccaaaac acgtcaccaa attaacttat cgaattcgac ctcattttcc
      601 aaagatggct attcctccaa caatactgga attactggca tagccggtga acatgatcag
      661 ttaaattatg gtatatatgt taatcagcaa caacaaaata atgatacctc gcttggtacg
      721 aatttaagct ggagaactcc catcgccata atagatggca gctatagtca ttctaaaaac
      781 gcctggcaaa gtggtggaag tattagtagt ggattagttg tctggcccgg tggtattaat
      841 atcactaacc agttatccga tacatttgca attctggatg cgcctgggtt agaaggtgca
      901 catattaatg gacaaaaata caatcgaaca aacagcaaag gccaggttgt ttacgacctg
      961 ataatacctc atcgtgaaaa ccatctggta cttgatatag caaacagtga aagtgaaaca
     1021 gaattgcagg gcaatcgtca aattattgca ccttaccgtg gagcagtttc ttatgtgcag
     1081 tttacaactg accaacgtaa gccctggtat atacaggcac tgcgtcccga tggttcgcca
     1141 ttaacctttg gctatgacgt actggatctc caggaaaata atattggagt cgttggccag
     1201 ggtagtcgcc tttttattcg cgtagatgaa attcccactg gaataaaagt tgctctcaat
     1261 gatgaacaga acttattctg tactattact tttcaacacg ttatcgatga aaacaaaaca
     1321 tatatatgcc agtaaaagga gtttactatg aagtggcttt tattaatcac cttaagtcta
     1381 tattctttta ttgtgcaaag tgctccctgt gcattgacca atgtcggtga acaacgtgga
     1441 acatatatac tcaagccact gagtatgaaa ggtaatctaa ccgccgaaaa attgaaaaca
     1501 ccggaaatat cattgagtgt acagacttgc aagagacaac caacgccatt aaagttcatt
     1561 ttttaacaca gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc
     1621 cctttgaatc tgacggtaaa aaaatagagc ttccagggaa gtcatcttat aatttagaag
     1681 aaatgctatc tgcgataaat ttcaccattc catattctat cgcatctgta tcagatagtt
     1741 ctaacaccgc agacattttt cctggaactc cttttccttt gttgttcgaa attgaagtga
     1801 gttcatgtac agggaataat actaattgta atagcgtccg ctttaattat aatttaaata
     1861 ctattttaca ggtaaattta atgacctgtg gttttgaaga tcaatccatc gacactggac
     1921 gatttgtact gtctcgtatc aataatgaaa attatcagtt tcatcatata agatttgact
     1981 gcatccaggg cgagcaaggt gaattagttt ttgaacccag tgatgttgaa tattactttg
     2041 aaccagtgac gatacaggag tcaggaacca gcatattaaa aaatgagtta gaaaacagtg
     2101 aggacggtgc gggtcaagta ggctttcaac tctccaatga tggtacgaat gaaatccagt
     2161 atggtaaaag caattactac tcttttcagc atccccatga aggaagtaat cagattcctc
     2221 tatttattcg cccgcgtaca tatggaaata atgtctcaag cgggcaaata atgagccgag
     2281 tcaaaatcgt ggttatgtac aactaatcta tctcccccac cttaactttg gggggaggtg
     2341 ccgattaacg cgccgctgga ctaaaaagcg cagaataatc cgtattgcag attcgctgta
     2401 ccgccgggtg ctgaatcatc cgctcagcaa aaatagcatg gtactcttcc atcacattct
     2461 cgacgcgacc aatttctacg acagttttat cggcataaaa gtcatatgca taaagcgttg
     2521 gggcaacgaa gattgcattg tgcatcgcac caaaagcttt catcaacgcg gcatcatcaa
     2581 actcgccgag gatttctacg tttaatccct gggagttaaa ccagttaagc aatttgcgcc
     2641 ctaacattga acgtcgccca ggaatcaaaa gtcgccgttc ttccagacaa gccgggaacg
     2701 gtttttctgg tggtggattt gtacaccaga aactcacgcc acattcgcca attctcacgg
     2761 agaacaggcc ttcctgctgc gtagagtcta tcggacagtc agaaatgatc atatccagtt
     2821 tatgctgact taattgctcc agcagcattt cgtgggtgga ttcgaagcag cgaagatgaa
     2881 tgggctcgcc ttctaccact gcggcgttaa gtacgctact gaccaggcgt ttggaaagtg
     2941 catcagccac accaacgtca aacaataaat tggattcttt gcgatagttc acaatatcca
     3001 gcatttcctg gcttaaggtg aacattttat cggcatagcg atagaccagt tctcccagct
     3061 cgctgggttc gagaccacgt cccttgcgtt taaataattt gccttgcagg cgctcttcca
     3121 gcgctcgaat ctgtccggta atggtttgtg gagttaaata aagcgcctcc gctgcgccaa
     3181 ccacggaacc ttctttatag acatgccaga agtaatacaa gtggttgtaa ttgatatgag
     3241 acatgctcat ttctctccct gataacaatg aaaagggagc cgtttatggc tccccggtaa
     3301 accgtcctgt caaactgatg gacgcaaacg aacgcgtaac cagctgtatc caattaccgc
     3361 cgaagagata gaaccgacca ggataccgag tttcgcccag ttaatcagtt ctggatctac
     3421 gctaccaaag gccaggctgg caataaagat agacatagta aaaccgatac cgcacaggat
     3481 ccccaccgcc ataatttgct gataagtcgt tccctcaggc agatgcgcca gtttcaaacg
     3541 cagcgccaac cagcagaaca gactaatccc cagcggtttg ccaatcagca agccagcgat
     3601 gatccccaat ggcagaatgg aggtcaagcc atccagcgtg acaccttgca gtgaaacgcc
     3661 agcattagca aatgcaaaca gcggcaaaat cagatacgcc acccacgggt gtaacacatg
     3721 ctccagtcgc ttcgctggag aacgcccatg cttctctttc aaaggaataa agaagccgac
     3781 aattaccccc gccagggttg cgtgaacccc cgatttcaac accgcagtcc acaacactac
     3841 gccaaccaga atatagacgc ccgtgcggcg tacaccacac agattcaata ccgcgagtac
     3901 cgcaattgct acagccgcga cgccaagaga ggccatcgat aagtcattag tgtagaacaa
     3961 tgcgatgata atgatggccc caagatcgtc gataatagcc agagccatca aaaagatctt
     4021 cagcgctaac ggaacacgac ttcccaacag cgccagcaca ccaagtgcaa aggcaatgtc
     4081 agtagccgcc gggatcgccc acccttcgcg ggtaatcgga tcggcatagt taaaggccag
     4141 atagagtaat gccggaacaa tcatcccacc aatagcggcg ataactggaa atgcggcctg
     4201 gcgtaagctg gctagcgatc cttgcatcag ttcacgttta acttccagac cgactaacag
     4261 gaaaaatacc gccatcagcg cgtcatttat ccataacagc atgtttttgt tgatttcgag
     4321 tgaaccaacc cggagctgaa ccggcgtctc cagaaagtcg tgataccatc cactggttgc
     4381 gccgctattg gccataatca tcgccaggat agcggcaatg ataagaataa tgcctcccga
     4441 ggcatcactg ctaaagaatc gatgcagatg tttcactttt tatttctctt tcaggtgaat
     4501 agatcgtttt tactattcta ccccgcacaa atcatcgtta aaatcagatt atagtgtagg
     4561 aataactcgt tttttacgat ccattgaaga atttgcgcca tcagcggtga gatgagtttt
     4621 tgacggctgt aagcgctcac gagtgaccct aagcccagat aatcatagct taaggtgagg
     4681 atttctgagg gattaattag gaaaagtaat aattaatttc ttgtcagtcg gcgttatttg
     4741 ttaggacaat atttcaaaaa taatccagct tgtcgtcaat aattgcgata aagagataaa
     4801 ttaaagttaa ataattaaat cattaaaaaa ttacgccaca aaaatgcgag gcgtcttcag
     4861 gattagaatc gataaaacag aatgggttaa agagagcgat ataatagcgg cgggtgcttg
     4921 aggctatctg tctcaggcat tagctgaacg gcagatagag aaaagccccg agtgatattt
     4981 tactatcaac ccgaggcccc ctatatgctg aacacatgta gagtgcctct tactgaccgt
     5041 aaggtcaagg agaagagagc aatgaagcag cataaggcga tgattgtcgc cctgatcgtc
     5101 atctgtatca ccgccgtagt ggcggcgctg gtaacgagaa aagacctctg tgaggttcac
     5161 atccgaactg gccagacgga ggttgctgtt ttcacggctt acgaatccga gtaagtgcaa
     5221 cggcggggag tgatccccgc cacttttcag gtgctcgcat atcttcaacg caccctaatt
     5281 ttacccaagc ctgcccacgg gcaggctttt ggggaggtta gcgggtcagg tcgtcaaaaa
     5341 acttcttcac accatcaaag aagctctttg agcgcgggct gttgtgctca ccggttgggc
     5401 caccgaagct ttcttgcagc tcttgcagca gctgtttctg cttctcgttc aggcctaccg
     5461 gtgtttcgac gacaacgcgg cacagcaaat caccctgtgc gccaccgcgg acagacttga
     5521 cgcctttacc gcgcatacgg aaaagcttac cggtctgggt ttcgccaggc actttcagtt
     5581 tgacgcgacc atcaagggtc ggtacttcga tttcgccacc cagcgccgcc atagcgaagt
     5641 tgatcgggac ttcgcaatag aggttgttgc cttcacgctc gaaaatcggg tgctgtttaa
     5701 cctgaacctg aacgtacaga tcgcctgccg gtgcgccatg ctcgcccgct tcaccttcgc
     5761 ccgcaagacg gatgcggtct ccagtgtcca cccctgccgg gattttaacg gacagcgttt
     5821 tgctgcgctc aacacgacca tgaccatgac atttgttgca cggatctttg atcagcgtac
     5881 cgcggccctg acagtgtgga caggtctgct gtacagcgaa gaatccctga cgcatctgca
     5941 cctgaccaga accatgacag gtcggacagg tctgcggctg tgtacctggt tttgcaccgc
     6001 taccgtggca aacgtcacac tcttccagag tcggaatgcg gatctctttg gtcacgccac
     6061 gtacagcttc ttcgagggtg agctccatgt tatagcgtaa atcagcaccg cgcgccgcac
     6121 gttgacgacc acgtccgccg ccaaaaatat cgccgaaaac gtcaccaaaa atatcgctga
     6181 agtctgcgcc gccgccaaaa ccgccgccgc ccataccgcc ttgctcaaac gcagcatgac
     6241 catactgatc gtatgccgca cgtttttgcg agtcggtcag aacttcataa gcttccttga
     6301 tctctttaaa tttcgcctcg gcctctttgt caccctggtt acggtccggg tggtatttca
     6361 tggccaggcg tttgtaggcc tttttgattt cacgctcttc cgctgttttg gaaacgccta
     6421 aaatctcgta ataatcttgc ttagccatct tttttaaatt gcccctagat gaatgcacgg
     6481 gcggagagga aattcccctt cgcccgtgtc agtataatta cccgtttata gggcgattat
     6541 tttttgtctt tgacttcttc aaattcagcg tcgacaacat cgtcatcttt cgcgttgttt
     6601 gcagaagcat cagcaccggc agtctgctgc tgggcatgtt gctgctgggc gatttccatc
     6661 agtttctggg aaacctgtgc cagctcctgc attttcgctt cgatagcggc tttgtcttca
     6721 cctttcagag cagtttccag tgcagtcagt gcagactcga tagcagtttt gtcgtcagcc
     6781 ggcagtttgt cgcctgcttc ttcaacctgc ttacgggtgc tgtgcagcag atggtcgccc
     6841 tggttgcgag tctgtaccag ctcttcaaac ttacggtcag cttcggcgtt agcttctgcg
     6901 tcgcgtacca ttttctggat ttcatcttcg ttcagaccag aagaagcctt gatggtgatc
     6961 ttctgctctt taccgctgtt tttatctttc gcggaaacgt gcaggatacc gtcagcatcg
     7021 atatcgaagg taacttcgat ctgcggcatg ccgcgcggtg ccgggttgat accatccagg
     7081 ttgaactgac ccagagattt gttatcagcc gcacgtttac gttcaccctg cagcacatgg
     7141 atggttaccg cagactggtt gtcttcagcg gtagagaaca cctggctgtg cttggtcggg
     7201 atagtggtgt ttttcgcgat cagcgtcgtc atcacaccgc ccatggtttc gatacccaga
     7261 gacagcgggg taacgtccag cagcagtacg tctttcacgt caccagtcag aacaccaccc
     7321 tgaacagcag caccgattgc tacagcttcg tccgggttaa cgtctttacg cggctcttta
     7381 ccaaagaact cagcaacttt cttctgaacc attggcatac gagtctgacc accaacgagg
     7441 ataacgtcgt cgatatcaga tacggacagg ccagcgtcct gcagtgcaac tttcagcggc
     7501 tcaatggaac ggtttaccag atcttcaacc aggctttcca gtttcgcacg agtcactttg
     7561 atgttcatgt gtttcggacc ggtcgcatct gcagtgatgt acggcaggtt aacgtcggtc
     7621 tgctgagcgg aagacagttc gattttcgct ttttctgccg cttctttcag gcgctgcatt
     7681 gccagcggat cgttgcgcag gtcaatgccc tgatctttct tgaattcttc aaccaggtag
     7741 ttgatcagac ggctgtcgaa gtcttcacca cccaggtggg tatcaccgtt ggtagccaga
     7801 acttcgaagg ttttttcgcc gtcaacttcg tcgatttcga taatagaaat atcgaaagta
     7861 ccaccaccca ggtcataaac cgcgatagta cggttgccag tacctttgtc cagaccgtaa
     7921 gccagcgcag ctgcggtcgg ttcgttgatg atacgtttta cttccagacc agcgatacgg
     7981 cctgcgtctt tggttgcctg acgctgagca tcgttaaagt atgccggtac ggtgataaca
     8041 gcttcagtta ccggttcacc caagtaatct tcagcggttt tcttcatttt tttcagcact
     8101 tcagcagaga tctgcggcgg tgccattttc tggcctttaa cttcgaccca tgcgtcgccg
     8161 ttatcagcag caataatttt gaacggcatg atggaaacat cacgctgtac ttcttcgtcc
     8221 tggaagcggc ggccaatcag gcgtttaatc gcaaacaggg tgttttgcgg gttcgtcact
     8281 gcctgacgtt tagccggctg accaaccaga gtttcaccat cctgggtata ggcaatgata
     8341 gaaggcgtgg tgcgatcgcc ttcggcgttc tccagcacgc gaggagtggt gccatccata
     8401 atcgctacac aagagttggt agtacccagg tcgataccaa ttattttacc catctaaacg
     8461 tctccactat atattcggtc atcatgtggt tgtgagtctg taataggggc gaaacgtctg
     8521 gtttcaactg cccaatttca tttttttgca gactcactca ctgcggttga ctactaaatg
     8581 gggtcgtaac ccacgtcatc aagggggaga tgcaaaaatt ttttgtgctt ttgcgcagaa
     8641 atttcgcgat atagcaggac aagacgtgaa actgacagca attttattca tataaagaat
     8701 gaatcgcagc caagacattc atcaaaaaat tgtaataaaa agaaaacatt acgcacctga
     8761 atcttctctt tatcagcagt aaactagtgg gtatccatcc ccctacctct ccccactaag
     8821 agaatcctta tgaaatccgt ttttacgatt tccgccagcc tggcgattag tctgatgctg
     8881 tgctgcacgg cgcaggcaaa cgaccataaa atcctcggcg tcattgcgat gccacgtaac
     8941 gaaaccaacg atctggcgct gaaacttcct gtttgccgaa ttgttaaacg catacaactc
     9001 tctgccgacc atggcgattt acagttaagc ggtgcatcgg tttatttcaa agccgcccgt
     9061 agcgccagtc agagcctgaa tattccttca gaaataaaag aagggcaaac cactgactgg
     9121 atcaacatta acagcgataa cgacaataaa cgctgcgtct caaaaatcac cttttccggt
     9181 catacggtga actcatcgga tatggccacg ctgaaaatta tcggcgacga ctaacgccag
     9241 aatatagaag ccacaaaaaa tgaatgttaa ttacctgaat gattcagatc tggattttct
     9301 ccagcattgc agtgaggaac agttggcaaa tttcgcccga ttgctcaccc ataatgaaaa
     9361 aggcaaaact cgcctctcca gcgtactgat gcgcaacgaa ctgtttaaat cgatggaagg
     9421 gcatcccgag caacatcgcc gcaactggca gctgattgct ggagaattac agcattttgg
     9481 tggcgatagt atcgccaaca aactgcgcgg acacggtaaa ttgtatcggg ccattttgct
     9541 cgatgtttca aaacgattga agctgaaagc cgacaaagag atgtctacgt ttgagattga
     9601 gcaacagtta ctggaacaat ttctgcgtaa cacctggaag aatatggacg aggaacataa
     9661 acaggagttt ctgcacgcgg tcgatgcgag ggtgaatgag ctggaagagc tgctgccgct
     9721 gctgatgaaa gacaaattat tggcaaaagg cgtgtcgcat ttgctttcca gccaactgac
     9781 ccgcatttta cgcacccacg cagcaatgag cgtacttggg catggtttgc tgcgcggcgc
     9841 ggggctggga ggccctgtag gtgcggcact aaatggggtt aaagcggtca gcggcagcgc
     9901 ctatcgcgtg acgattccag ccgtactgca aatcgcctgc ctgcgccgga tggttagcgc
     9961 cactcaggtc tgataccgta aaccggggaa aattttcatt ttcccctcaa aagatcgtag
    10021 acactgcccc actggctgat tattatgccg cgccctgaaa acactacagt tattcaggga
    10081 aattatttca ccattcattc gatgatgatt tttgaggaat tatgggcaac actaagttgg
    10141 ctaatccggc accgctgggc ctgatgggct tcggcatgac caccattctg cttaacctgc
    10201 acaacgtggg ttatttcgct ctggacggta ttattcttgc catgggcatt ttctacggcg
    10261 gcatcgcgca aatttttgct ggtctgctgg agtacaaaaa aggcaacact ttcggtttaa
    10321 ccgcattcac ctcttacggt tctttctggc tgacgctggt tgcgattctg ctgatgccga
    10381 aactgggtct gaccgatgcg ccaaatgcac agttccttgg tgtctacctg ggtctgtggg
    10441 gcgtatttac gctgtttatg ttcttcggca cgctgaaagg cgcacgcgtt ctgcaattcg
    10501 ttttctttag cctgaccgtg ctgtttgccc tgctggcgat cggtaacatt gccggtaacg
    10561 ccgcaatcat ccactttgcc ggctggattg ggctgatctg cggtgccagc gcaatctatc
    10621 tggcgatggg tgaagtactg aacgagcagt ttggtcgcac cgttctgccg attggtgaat
    10681 cccactaatt gttttgagat ccccccgctc ggggggattt ttttattcgc taacgtcgcg
    10741 tcttgcactc ttcggtctga atgctgcaac cacttccggt gccgtttcaa cgtatggccc
    10801 ttccagcaac tgaatgcagt acggtacgct ggcaaaaata ccgtgtacca caacgttacc
    10861 cgcagcgtcc ttcacacctt ccagcgtctc tttaatagac ttcggctgac cgggtaagtt
    10921 aaggatcagc gcctgtttgc gaatcacacc aacctgacgc gaaaggatcg cagttggtac
    10981 aaaatgcagg cttatctggc gcatctgttc accaaagcca ggcatctcgc ggtccgctac
    11041 tgccagcgtc gcatcgggcg ttacgtcacg acgcgccggg ccagttccgc ccgtggtgag
    11101 caccagatgg caactcattt catccactag ctcacacaac gtttgctcga tgatcgcctg
    11161 ctcatcgggg attaagcggg tttccagttc aaacggcgtg gttagcgccg atgtcagcca
    11221 ttcttccagc gcagggatgc ctttatcctg ataaacgccg ctggatgcgc gatcagagat
    11281 ggaaactaag ccaatacgta aagtattcat caagattttt ccgtttaaac agtctcgtta
    11341 aacagaatga tacactgcga agggagtgac agacaggctt cagaagaggt agcgtgaccg
    11401 acttcccggt cacgctaaga atgattacag cagatcgccg atcatttttt ccagtttttc
    11461 ctggtcaata gcaaacttac ggataccttc cgccagttta tctactgcca ttggatcctg
    11521 gttgtgctgc cacaggaact cggactcagt gatacgcgcc ggacgcgctt tcacttcgcc
    11581 ggtgtaagac agtttacgtt cgatagcccc ttcgctctca gccagctctt tcagcagtgt
    11641 aggtgcgatg gtaagacggt cgcagcctgc cagttccaga atttcgccga tgttacggaa
    11701 gcttgcgccc ataaccacgg tttcataacc gtgctctttg tagtactggt agatttcaga
    11761 tacagaaacc acgcccggat cttctgccgg agcgtactct ttcttatcag tattcgcttt
    11821 gtaccagtca agaatacggc caacaaatgg cgagatcagg aacacgcctg cttccgcaca
    11881 agcacgagcc tgagcgaagg agaacagcag ggtcaggtta cagttgatgc cttctttttc
    11941 cagctgttct gcagcacgga taccctgcca ggtagaagcc agtttgatca gaatacgatc
    12001 gttgctaata ccagcatcgt tgtagagttt gatcaggcgt tttgctttcg caattgacgc
    12061 ttcggtgtca taggaaagac gcgcatcaac ttcagttgag atacgacccg gaaccagttt
    12121 caggatttcc agaccaatat ttactgccag tttgtcggtc gcgtccacga tctgctgcgc
    12181 gcgatcgttg ctctgctgtt tcgcccaggc gacagcatca tcaatcaact tacggtattc
    12241 cggaatctgc gctgcgttaa gaatgagaga agggttggtt gtggcatcct gcggttgata
    12301 cagcttcatt gccgcgatgt ccccagtgtc ggccactacg gtggtgtact gacgaaggga
    12361 ggtcaatttg tccgtcatga tagtatttct ctttaaacag cttgttaggg ggatgtaacc
    12421 ggtctgccct gatgatatca cgacgcaacg tcagcgcaac cgccgacaag acggttatcg
    12481 ccaggcgaga ctgtttcgga tttctgacac tgcgttgata tcgcccgcca tttttataca
    12541 aaacctcatg tatgctacgc agaagttatc aagtacctcg tagcgtatat acttcttaaa
    12601 caattggtaa cgtttacaca ggaaagtcat cgcgaccggc aataagaggg atatgcatgc
    12661 cagatttttt ctcctttatt aacagcgtcc tttggggatc ggtaatgatt tacctgctct
    12721 tcggcgcagg ttgttggttc acttttcgca ccggatttgt gcagtttcgc tacatccgcc
    12781 agtttggcaa aagtcttaaa aatagcattc atccacagcc aggcggttta acctcatttc
    12841 agtcattgtg taccagtctt gcggcgcgcg tgggtagcgg caacctggcc ggcgttgcgc
    12901 tggcaattac cgccggtgga cctggagccg tcttttggat gtgggttgcc gcgtttatcg
    12961 gcatggcgac ctcgtttgcc gaatgttctc tggcacaact ttataaagaa cgtgacgtca
    13021 atggacagtt tcgtggcgga ccggcatggt atatggcgcg cgggctgggg atgcgttgga
    13081 tgggcgttct gttcgccgtc tttttgctca tcgcctatgg cataattttc agcggagttc
    13141 aggcgaacgc cgttgcccgc gccctaagtt tttcttttga ttttcctccg ctggtgacag
    13201 gcattattct cgctgtcttt gctctgctgg caatcactcg cggtcttcat ggcgtcgccc
    13261 ggctcatgca ggggtttgtc ccgttgatgg cgataatctg ggtgctgacc agcatggtga
    13321 tttgcgtaat aaatatcggg caacttcccc acgtcatttg gtctattttt gagagtgctt
    13381 ttggctggca ggaagcggca ggcggcgcgg cgggatatac cttaagccag gcgattacta
    13441 acggttttca gcgcagtatg ttttccaatg aggcgggaat gggttcgacg ccaaacgcgg
    13501 cagcggcagc ggcgtcctgg cctccgcatc cggcagcgca agggattgtc cagatgattg
    13561 gcatttttat cgacaccctg gtcatctgta cggcaagcgc catgctgata ttactggcgg
    13621 gtaacgacac aacctacatg ccgctggaag gtattcagct tatccagaag gcgatgcggg
    13681 tgctaatggg ttcctggggt gctgagtttg ttaccctcgt ggttattctg tttgccttca
    13741 gctccatcgt tgccaactac atttatgccg aaaacaatct cttcttttta cgcctgaaca
    13801 accctaaagc gatctggtgt ttgcggatct gcaccttcgc aacggtcatc ggcggcacct
    13861 tgctaagcct tccgctgatg tggcaactgg cagatatcat aatggcctgc atggctatta
    13921 ccaatttgac cgccatttta ctgctctcgc ctgtggttca taccattgcc agtgattatc
    13981 tacgccagcg taaactcggc gtgcgcccgg tgtttgatcc gttgcgttat ccggatatcg
    14041 gtcgccagct ttctccggac gcgtgggatg atgtttcgca ggagtaatca caactatcga
    14101 tcaactcatt ctcttttttt gctaaagtcg gcataaattt cctgcaagga ctggatatgc
    14161 tgattcttat ttcacctgcg aaaacgcttg attaccaaag cccgttgacc accacgcgct
    14221 atacgctgcc ggagctgtta gacaattccc agcagttgat ccatgaagcg cggaaactga
    14281 cgcctccgca gattagcacg ctgatgcgca tcagcgacaa actggcgggt atcaacgccg
    14341 ctcgctttca tgactggcag ccagatttca cgccggagaa tgcccgccag gcgattctgg
    14401 cgtttaaagg tgatgtctac accggcttgc aggccgaaac cttcagcgaa gacgatttcg
    14461 attttgccca acagcatttg cgaatgcttt ccggcttgta tggcgtactg cgcccgctcg
    14521 atttaatgca gccttatcgt ctggaaatgg ggatccgtct tgagaatgcc cgagggaaag
    14581 atctgtatca attctgggga gatatcatca ccaacaagct gaacgaggcg ctcgcagcac
    14641 aaggcgataa tgtggtgatt aatctggcgt cagatgagta ttttaaatcg gtgaagccga
    14701 agaaattgaa tgccgagatt atcaagccgg tgttcctcga tgagaagaac ggcaaattta
    14761 agataatcag cttctacgct aagaaagcac gcggtctgat gagtcgtttt attattgaaa
    14821 atcggctgac caaaccagag caactgactg gttttaatag cgaaggttac ttctttgatg
    14881 aagcttcctc cagcaatggc gaactggtgt ttaaacgcta cgagcagcgt taagtttgta
    14941 gtcaatgccg gatgcggcgt gaacgcctta tccggcctac aaaagcatgc agattcaata
    15001 tattgcaga
//
LOCUS       DAZGSP010000120        14874 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZGSP010000120 DAZGSP010000000
VERSION     DAZGSP010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.121"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(614..1150)
                     /gene="yfdR"
                     /locus_tag="ACHK74_004232"
     CDS             complement(614..1150)
                     /gene="yfdR"
                     /locus_tag="ACHK74_004232"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP0222949.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVMPLAPGHA
                     YGMFMERFNELSELRTYA"
     gene            complement(1279..2103)
                     /gene="yfdQ"
                     /locus_tag="ACHK74_004233"
     CDS             complement(1279..2103)
                     /gene="yfdQ"
                     /locus_tag="ACHK74_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFP0222950.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDTIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            complement(2169..2531)
                     /gene="yfdP"
                     /locus_tag="ACHK74_004234"
     CDS             complement(2169..2531)
                     /gene="yfdP"
                     /locus_tag="ACHK74_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFP0222951.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            complement(3311..4003)
                     /locus_tag="ACHK74_004235"
     CDS             complement(3311..4003)
                     /locus_tag="ACHK74_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP0222952.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQALSNIARSLGVDIADLFTSDFKSNTVCKNSTGEDVAQVKDVFRIEMLDVSASAGNG
                     LIQGGDVIDVIHAIEYITDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDIS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            4101..4361
                     /locus_tag="ACHK74_004236"
     CDS             4101..4361
                     /locus_tag="ACHK74_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0222953.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIDL
                     AERLANFFKGEISEMQILYPARFQSSQNRNELKPQEKEVSRG"
     gene            4354..4905
                     /gene="ymfL"
                     /locus_tag="ACHK74_004237"
     CDS             4354..4905
                     /gene="ymfL"
                     /locus_tag="ACHK74_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFP0222954.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAAEWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAAGTHYIADAVAQSAGGVFVSLPDIEEVENADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEQTAMNDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            5051..5146
                     /locus_tag="ACHK74_004238"
                     /pseudo
     CDS             5051..5146
                     /locus_tag="ACHK74_004238"
                     /inference="COORDINATES: protein motif:HMM:NF025339.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            5100..6065
                     /locus_tag="ACHK74_004239"
     CDS             5100..6065
                     /locus_tag="ACHK74_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP0222955.1"
                     /translation="MLFVGSQKHSALSIFAKTTRMSALVLCGNSGVILLSVKDHQHID
                     SAIPGRYTVQAPYKTGAGIGVLKLITEHNRAHAVFSCHEHYYAQIMVGRTGPTSVGPG
                     SVVSGNANPVRLTTSVIGVPCGEFSKKLTTGAVTMTTLPTLSQPEIAIVDGQAVTSSL
                     AVADFFSKRHDDVLKKIRILDCSPEFCARNFAETSILVRQPNGGTRKLPCYQITRDGF
                     AFLAMGFTGKRAARFKEAYINAFNQMEKQLSKPSTLSDAAHNASVLYSHLSVIHKVWL
                     QQLYPMLAKVESPLAISLYDHINDAAAISSLINMTLNPSEVRGRK"
     gene            6062..6286
                     /locus_tag="ACHK74_004240"
     CDS             6062..6286
                     /locus_tag="ACHK74_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0222956.1"
                     /translation="MIRNIFKRFTDQTFRCPRPGQWYTTPAGHVLRVSLVDRECQKVI
                     CEPLGRNYRVSMPLIAFCSGKMFKRLGGVA"
     gene            6283..6582
                     /locus_tag="ACHK74_004241"
     CDS             6283..6582
                     /locus_tag="ACHK74_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0222957.1"
                     /translation="MNCFQFVCGCAFDNPIQRLIMLRVLMSGSSDGEGERVIDHQVLA
                     DFCCCSKQAIFRETLALERAGYLHIRKIATLTIDAKARLQPARGYTILMLRKEVV"
     gene            6579..7562
                     /locus_tag="ACHK74_004242"
     CDS             6579..7562
                     /locus_tag="ACHK74_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP0222958.1"
                     /translation="MSRYAPTPEVMAIGQINISGNVTPATWWKYIRLPSGRPDATAIA
                     LLSEIVYWYRPTEVRDEHTGALLGYRKRFQGDKLQRSYQAFAEQFGFGKRETADALKR
                     LRDAGFITLDLRTVEMLDGVKCSNILFVGINPQAIAAITTPSSVSPESNSNNAISDTA
                     ITLKRNTPRRHNGTGDTPNVDTNTEITTEITTETKNTIDASADASAPARSARQEYSPE
                     FEQAWQEYPKRAGGNSKSAAFKAWKARLNEGVNPETMLEGVKRYAGWVSAMGNSGTQF
                     VKQAVTFFGPDRHFEESWEVPAVSAARREDPYFKASYDNVDYSQIPAGFRG"
     gene            7559..8053
                     /gene="yfdN"
                     /locus_tag="ACHK74_004243"
     CDS             7559..8053
                     /gene="yfdN"
                     /locus_tag="ACHK74_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0222959.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAVSGDEWYLSGN
                     YVGA"
     gene            8053..8706
                     /locus_tag="ACHK74_004244"
     CDS             8053..8706
                     /locus_tag="ACHK74_004244"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFP0222960.1"
                     /translation="MSNKYCQELVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCTAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            8703..9029
                     /locus_tag="ACHK74_004245"
     CDS             8703..9029
                     /locus_tag="ACHK74_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFP0222961.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTVVKDDDSEAVGIIRSLLAGEENARLRAAHWLH
                     ERGLKV"
     gene            9026..9421
                     /locus_tag="ACHK74_004246"
     CDS             9026..9421
                     /locus_tag="ACHK74_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP0222962.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKASCKLATR"
     gene            9584..10399
                     /locus_tag="ACHK74_004247"
     CDS             9584..10399
                     /locus_tag="ACHK74_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFP0222963.1"
                     /translation="MNQLLVIDGVSVRQYFESNYCLNDLQKAALLAAGENRSSRSLEV
                     HEFMRRPETKALVELLEEETTGDSRSIPVITIQGRNGGTYVCKELVYAYAMWISPAFS
                     LKVIRTFDALHNSSPEETTSDKIKSGVILLESAAKTLNLSNSSKLGAYQKLSKVAGLP
                     ELMPIYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYS
                     PTAINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            10407..11396
                     /locus_tag="ACHK74_004248"
     CDS             10407..11396
                     /locus_tag="ACHK74_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0222964.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPNLVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            11410..12162
                     /locus_tag="ACHK74_004249"
     CDS             11410..12162
                     /locus_tag="ACHK74_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP0222965.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAESKARCERCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGAPVYKICGRCNGNRFSRLPTTLARHRVQKLIPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEEQLRKVTR"
     gene            12311..12387
                     /locus_tag="ACHK74_004250"
     tRNA            12311..12387
                     /locus_tag="ACHK74_004250"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12345..12347,aa:Arg,seq:tcg)
     gene            12401..12477
                     /locus_tag="ACHK74_004251"
     tRNA            12401..12477
                     /locus_tag="ACHK74_004251"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12435..12437,aa:Arg,seq:tct)
     gene            12569..13528
                     /gene="stxA2b"
                     /locus_tag="ACHK74_004252"
     CDS             12569..13528
                     /gene="stxA2b"
                     /locus_tag="ACHK74_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFP0222966.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRSVNE
                     EIQPECQITGDRPVIRLNNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            13541..13804
                     /gene="stxB2b"
                     /locus_tag="ACHK74_004253"
     CDS             13541..13804
                     /gene="stxB2b"
                     /locus_tag="ACHK74_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFP0222967.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
     gene            14323..>14874
                     /locus_tag="ACHK74_004254"
     CDS             14323..>14874
                     /locus_tag="ACHK74_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP0222968.1"
                     /translation="MTFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVLVKPSDGEGTVISTTSEPEYYFVVVLAGQSNSMAYGEGLPLP
                     ETYDRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQ
                     YGTVGQGLHIAKKLLPFIPANAGI"
BASE COUNT         3727 a         3443 c         3839 g         3865 t
ORIGIN      
        1 cgttaatttt caatatgaaa ggaataatga gtatttctgc ttttactcta aagttgttcc
       61 gccgaaattt catcatttct ttgtgttgga tttttctgat ggcaagataa atattgatag
      121 agaccaggat ctgatgactc agatggcatt gtgcatgggg tattataatt attaatattt
      181 cgcctgatac caactcaaaa tcttttttgt cgcatccatt gtggattaat attcagaggt
      241 gaggagtgtc tgcggaattt atattttgca ggatgagcat acaatacatg atataacatc
      301 tgtttttggc acagagcata tagtcagatt atgtttagtt ctgtgccata gatggatact
      361 atctcacctc ttggctaact ctcattactc aataactcca gcaaatctgt atattttgcg
      421 tgatgcccat ttatttgggc aggatttaat atcaggatct ggaaaatctg gcctgtattt
      481 ctggcctgtc ctcctgttta cgctgttcca gcgaagcacc gtcgatattg aaacgccaca
      541 gaattcggcg acttgttttg ttgtcattaa gttgctcatt gctacccctc cagcggttgt
      601 tcagttgaaa gactcatgcg tatgtgcgta actccgataa ctcgttaaag cgttccataa
      661 acatcccgta ggcatggccc ggtgccagtg gcatcacgtt gaacatctct gttgccggga
      721 taccttccag tactggccag aaagagccat catcaagccc gagatcgcgg cgttcggttg
      781 ccagcatgat gagatcggca tatttcacag gcgtgctcat aaccgggggt aacccgtatt
      841 tctcacggat tacggcgtct attttttctt ccatccgttt atagtcagga agaaggcgtt
      901 tcagtggagc gggaatatcc tggcaatacg cttctgttgc atcatgcatt aacgcttcaa
      961 aagcaaattc ctgcggcacc agctggctgc aaagcaccgc atgctgggcg acactgtaga
     1021 agtgtgaaag atgtcctgca aagcgacaga tatttgaaag ggaaactgcg atatcgttaa
     1081 tcacgatgtc gtctttattt atcctgtcat aataaaaatg cttcccggaa aaagttttaa
     1141 taaatgacat ttcgttctcc actttatatg cgctgcaccg cgttgaattc tgctaaaagg
     1201 aagctctcac catccggtga ttattgagtt aattacgttt ccataaatgc ccccgcaggg
     1261 gcatttgcag taatgaaatc aggcggtgaa agtaccaata aaggtttcta ctttgctgtc
     1321 tttgaatttc tcaacaagca gatcacgaaa ttcgttagcc atttcttcct gcaccgcttc
     1381 cagctgaata atgcgcagaa ccagtacagg acgatcgcca gtgataatgc tgaggcgtaa
     1441 tttaaacgga cgttctttca ggccttcaaa cggaacgcat ttaaattcaa atgccactgg
     1501 cataatgtct ttggttttcg cttcgacaga ctccatcaga gagcgtttgc cgctgaagtc
     1561 attgtcttca aaatcagcgg tctggtttgc ttcaattgtg attttacgga ctgccgcagc
     1621 cgctttggtt gcctgaatgg tgtcaccatt agcatcaaag cccacaaggt agtcggccca
     1681 gtcttcaatc cattctgcca gtgacttctg ggagttacgc tcgccattaa cagacagcag
     1741 ggcagagaac ggtgctgtct ttttcagttt gagagtggcg gtgttatctg cgtgacctgg
     1801 ttcatcaata gtacctaggt taagcacact gacggctcgc atattatcgg catcgataaa
     1861 gcagcgggtg ccttcatctg caagatcttt agaataacgg gtaaagtcat cgatgctggc
     1921 agtggaaagc gcaccacgga aacggaagcg atttaaatta aatttttcca gatcatgaat
     1981 gcggaaattc tcaggcaatg ccacagcatc ggcaccaatc ttactgataa tttcattaac
     2041 accctgagca gaaataaggg catggatttg attaattgcg gttgcgtcta agttctgaga
     2101 cataataagt cctcactata ttaagatatt cagtgatgag ataaataatc agttaattaa
     2161 gaacgatatt aatgacctgc tgcgcggagt tttccgtcag gctcaccggc aagagtcagt
     2221 aattgtccct ggtcttcctg cagaatagtc aggcgaccac cgcgattgac atacatcggc
     2281 gtttcggtgg tgtcttcttc ggaaattttc ccgcggttag tcgggcgaac atatgagagt
     2341 ttgtgtttga ttttcacacg gttctcatca aacggttcga tttccaggtt gagcgagacc
     2401 ttacctttgg ttttcgtgtt catcacaccg gaagcgactt cactgagaac tgcgccgatt
     2461 tttgtttcaa atacgccgcc gtccagctcc ccgataaatg cctgcacatc agtactgcgt
     2521 tcgctagcca ttttgttgct cctcatcata tcgaccctgc aaggtcggtt ggtttctcca
     2581 caaaacagag aagaacacct gcggtggcag ccgcccggat ggattgggtt atgagcccgt
     2641 cgtccggtga tgctcttctc tgttttgtaa aaagagcggt accagccgga agcaagtgta
     2701 caaactggta ctgccaaagc agtggctgtt gtggtgaccg gtgctgatct ccggcttgcg
     2761 gttatttcag actctcacgg gcgtttgatt gccccgccga acagctcttt tccgcaatag
     2821 ctgcaatgtc tttcgcgcat cagcctgcgc attcaccaca acgctgagag cattgccggt
     2881 gtccgaatcg aacggacctt ttccctgccc aaccctccca tctgaatggg actgtctgga
     2941 attgaaccag cacttatgcc ttgctcgtca atgctctcat cgttgcgtcc tggtctcttc
     3001 ccaggcgtca aaccgaatcg ccacgctggt taggcgtctt atcagcatcc tcattgactt
     3061 gcacattccg gctacctggt ttgtttgccc gagcaaggag tggattgtcc tctttaacgt
     3121 ccccagaccg ctaacgacgc atgtgccata cgccgtgtta caaccaaatt ttgttaggac
     3181 cttgtttgtt ggtctggaaa gaaagataaa atgaaattgc gcattatgca agtgtttttg
     3241 ttgcgagatg cgcaatatga tgggtgatga aaagccacct tctggtggct aattgatgag
     3301 gaggtcgggg ttaattgtgt cgcttaaggg tttgtgactg actgattaag accttcccaa
     3361 agaccataaa ccggtgttcg ttttcgctgg taattcccca ttcacggtaa atctggttat
     3421 cagaaatcac cagtagtttg tcaggtatca tttgcagtcg tttgacataa attttatcat
     3481 caaaaccaaa cacatagata ccatccccat caaactgatt gatactgata tcaacgaaga
     3541 tgagatctcc tggctcaatg gttggacaca tactgtcccc acgaacgttg ataactttaa
     3601 tgtgattggc tggtcgtcct ccaaacatcg atacagcatt atcagttatg tattcaatgg
     3661 catgaatcac atcaatgaca tcaccgccct ggataaggcc atttcccgca ctggcactga
     3721 catccagcat ttcaatacgg aatacatcct tcacctgcgc aacatcctca ccagtactgt
     3781 ttttacatac agtattactt ttgaagtctg aggtaaagag atctgcaata tcaacaccta
     3841 agctcctggc aatattactc agggcttgtt cagtgaattg tttctgctta cctgtttcca
     3901 ggcgtgagat attcgccgca tccactccta ttgcttcagc gagatcggcg attttcatgt
     3961 tcttcgcctg gcgaagttgt ctgactcgat ttcctatgtt catgcgttta ttacatttct
     4021 ttattgcgcg ttaagcaaat caacttgcgc aaaatatttg cgtgaaataa tatgcttatc
     4081 acgcaatatg tggaggttat atgcaatcac cattacgaaa tgtgcgtaag gcgcacggat
     4141 ttactttgca gcatgttgct gcgggtgttc aggttaatcc agcgacgctg agtcgtattg
     4201 agagactgga acaaattcca tctatcgatc tggcagagcg tctggccaat ttttttaagg
     4261 gagaaatcag cgaaatgcag attctctatc ctgctcgctt tcaatctagt caaaaccgga
     4321 atgagttaaa accacaggaa aaggaggtaa gccgtgggta agcatcactg gaaagtggaa
     4381 aaacagcctg agtggtacgt gaaagcggtc agaaaaacta tcgcggcgtt gccgggtggt
     4441 tacgctgaag cagctgagtg gctggatgta acagagaacg ctttattcaa tcgccttcgt
     4501 gccgatggcg atcagatttt cccgctggga tgggcaatga ttttacagcg cgcggctggc
     4561 actcactaca ttgcggatgc tgtcgcacag tctgctggtg gggtgtttgt atcgcttcct
     4621 gacattgagg aagtagagaa cgccgatata aaccagcgcc tgctggaagt catcgaacag
     4681 atcgggagtt actcaaagca gattcgttcg gcaatcgaag atggggtagt ggagccacac
     4741 gagcagacag caatgaatga tgagttgtat ctgtcaattt cgaagctcca ggagcatgcg
     4801 gcactggtct acaaaatttt ctgcgctcca gaaaagagtg acgcccgcga gtgtgcagct
     4861 ccgggcgtcg tggcgttttg tgtctgtgga gaaactaacg catgaacagt ttaacggcaa
     4921 ataaccgttt gtcgcaacag ctggtggtca gtgtcgctgc acacctgttg ttacggcatg
     4981 aatgcagatt accaaatcac ctggctgtaa gtaaccacag agaactttac ctgactgtgg
     5041 ggggcgagtt gtgcaggaac ttaaccgctg gtttcgtgac ggaagaggac tttatgttca
     5101 tgttattcgt tgggagccag aaacacagcg cgttatctat cttcgcaaag actacccgca
     5161 tgagtgcttt agtcctttgt ggaaattcag gcgtgatttt gttgagtgtg aaggaccacc
     5221 agcatattga ttctgcaatt ccgggacgtt acactgttca ggcaccttat aaaacgggtg
     5281 ccgggattgg cgtcctgaaa ttgattactg agcataaccg cgctcatgcg gttttttcgt
     5341 gtcatgagca ttactacgcc caaattatgg tggggcgtac agggccgact tcggtcgggc
     5401 cgggttcggt agtctccggt aacgccaacc ctgtacgtct caccacctct gtgattggcg
     5461 tcccatgtgg tgagttttct aaaaaactga ctaccggggc tgtcactatg actactctcc
     5521 caaccctctc tcaacctgaa attgccatcg ttgatggtca ggctgttaca tcttctttgg
     5581 ctgttgctga ctttttctct aaacgtcatg acgatgttct gaaaaagatc cgcattttgg
     5641 attgttctcc agagttttgt gcccgcaatt ttgcggagac atcaattttg gtacgccagc
     5701 ccaacggcgg tactcgcaaa cttccttgct atcaaataac ccgcgacggc ttcgcgttcc
     5761 tggcaatggg cttcactggc aaacgcgccg cccggtttaa agaggcgtac atcaacgcct
     5821 ttaaccagat ggaaaagcag ctttcaaagc catccacgct gagcgatgcc gcacataacg
     5881 ccagcgtgct ttactcccac ctgtcggtaa tccacaaggt ctggctgcaa caactctatc
     5941 ccatgctggc caaagtggaa tctccgctgg ctataagcct gtatgaccat atcaacgatg
     6001 ctgctgcgat ttccagccta atcaatatga cactgaaccc ttcagaggta agggggcgca
     6061 aatgatccgg aatattttca aacggtttac cgatcagact ttccgttgtc ctcgcccggg
     6121 tcagtggtac accacgcctg cagggcatgt tctacgtgtt agcctggttg accgtgaatg
     6181 tcagaaggtg atttgtgaac cgctgggccg taattaccgc gtcagtatgc cgcttatagc
     6241 cttttgctcc ggaaaaatgt ttaagcgtct gggaggtgtg gcgtgaactg ttttcagttt
     6301 gtgtgcggat gtgctttcga taacccgatt cagcgcctga ttatgttgcg tgttttgatg
     6361 tcgggttctt cagacggtga aggcgagaga gttattgatc atcaggtgct tgctgatttc
     6421 tgctgttgtt ctaagcaagc gatattcagg gaaaccctgg cactggaaag agctggttat
     6481 cttcatatcc gaaaaattgc aacgcttact attgatgcaa aagccagact acaacctgcg
     6541 cgtggctaca caattctcat gctgcggaag gaggttgtat gagccgttac gcccccacac
     6601 cggaagttat ggctattggt caaattaata tttccggcaa tgttacacct gcgacctggt
     6661 ggaaatatat tcgactaccc agtgggcgtc cggatgcgac ggctatcgct ctgctttcag
     6721 agatcgttta ctggtaccgc ccgacagagg tcagggatga gcacaccgga gcgttgctgg
     6781 gatatcgcaa gcgttttcag ggcgacaaac tgcaaagaag ctaccaggcg tttgctgagc
     6841 agtttggttt cgggaaaagg gaaaccgcag atgcgctgaa gcgtctgcgc gatgcagggt
     6901 ttattactct ggatttacgc acggtggaaa tgctcgatgg ggtgaaatgc agcaatattt
     6961 tgtttgtcgg gatcaaccca caggcaattg cggccatcac cacaccttct tctgtttcgc
     7021 cagaaagtaa cagcaataat gcaatcagcg atacagctat tacgttaaaa cggaacaccc
     7081 cccgacgtca taacggaaca ggggatacgc cgaatgttga tacaaataca gagattacta
     7141 cagagattac aacggagact aaaaacacta ttgatgcatc cgctgacgcg tctgcgccag
     7201 cgcgttctgc ccgacaggaa tattcaccgg aatttgaaca ggcctggcag gaatatccca
     7261 aacgtgctgg tggcaattca aaatcagcag ccttcaaagc ctggaaagcc cgtttgaacg
     7321 agggggtaaa ccccgaaacc atgctggaag gtgtgaaacg ctacgcgggc tgggtatctg
     7381 cgatgggcaa tagcggcaca caatttgtga aacaggctgt cacgttcttt ggtccggatc
     7441 gtcatttcga agaatcctgg gaagttcctg cggtatctgc agccagacgc gaggacccgt
     7501 acttcaaagc cagttacgac aacgtggact acagccagat cccggcagga ttcagggggt
     7561 gatcatgagt cttttgaatg acgttcagaa attcattgaa gcccatccgg ggtgtacttc
     7621 cggagacatt gcggatgctt ttgcaggtta ctcacggcag cgcgttctgc agtcagcaag
     7681 caagttacgt cagagcgggc gtgtggctca ccgttgtgaa ggagatacac gcagacattt
     7741 cccgcgcctg actgagagag cgcaggaacc ggaaccacaa ccagttcgtg aaaccagacc
     7801 tgtgcgcaat ttctatgtcg gcactaacga tccccgggtg attttgtgcc tgacccgcca
     7861 ggctgaagaa ctggagtcca ggggcttata ccgtcgtgct gcaaccgtgt ggatggcggc
     7921 attccgtgaa agccactccc agccagaacg aaacaatttt ctggcacgtc gtgagcggtg
     7981 cttacggaaa agcagcaagc gcgctgtatc gggtgatgag tggtatctgt cagggaatta
     8041 cgtgggggct taatgagtaa taaatattgc caggagctgg tggagctgcg gaacaaacca
     8101 gcccatgaac tgaaggaagt gggcgatcag tggcgcacgc cggataacat tttctgggga
     8161 attaacaccc tgtttggccc atttgtcctg gatctgttta ctgacggtga taacgccaaa
     8221 tgtaccgcgt attacacggc ggaagacaac gcgctggcgc atgactggtc agaacgtctt
     8281 gcggagctta aaggtgctgc ctttggtaat cccccataca gccgcgccag tcagcatgag
     8341 gggcaataca tcaccggcat gcgttacatc atgaaacatg ccagtgccat gcgtgataaa
     8401 ggcgggcgct atgttttcct gatcaaagct gccaccagcg aagtgtggtg gccggaagat
     8461 gcagatcata ttgcttttat tcgcgggcgt attggttttg aactgcctgc ctggtttatc
     8521 ccgaaagatg agaagcaggt gccgacaggc gctttcttcg ctggtgctat tgctgttttc
     8581 gacaagacct ggaagggacc ggcaatcagc tacatcgggc gtgatgaact tgaggcatgt
     8641 ggtgaggcgt ttctggcgca ggttcgccag caggcggaaa aactggtcag ggagatggcg
     8701 gcatgacgac gttaactcaa tgccagcagc aggtgctgga tatgctgatt tcttatcaga
     8761 aagaacgtgg cttcccgcca accaatcagg aggtggcaac catgctggga taccgttcag
     8821 tgaatgcagc ggtggagcat cttcgcgcac tggagaaaaa aggcgtcatc acgataaagc
     8881 gtggtgtggc ccgggggatc acgcttcata ccgtggtgaa ggacgacgac agcgaggcgg
     8941 tcgggattat ccgctcactg cttgccggtg aggaaaacgc caggctgcgt gcagcccatt
     9001 ggttacatga gagaggcctg aaagtatgaa gctgatcctg ccttttccgc ccagcgtgaa
     9061 cacgtactgg cgacacccca acaaaggggc gtttgctggt aagagcctga taagcgcggc
     9121 ggggcgaaaa ttccagagcg cggcgtgtgc agcaatagtt gagcagttac gtcgtctgcc
     9181 gaaaccaacg tcggcacctg cttcagtgga gatcgtgttg tttcctccgg ataaccggat
     9241 ccgcgatctg gacaactata acaaggcgct gtttgacgcc ctgacccacg cgggggtgtg
     9301 ggaagacgac agtcaggtga aaagaatgct ggtggagtgg ggaccggtta tcccggaggg
     9361 gaaggtcgag atcactatca gtaagtacga aaaagcgagt tgcaaattag caactcggta
     9421 acggaattga gcaacaccct aaatttgggt attacctcgt taaagatact gtatttatga
     9481 acagtgtatc cttgataact attaaaaatc gcagtaagtt catcctgcat caacgaaaag
     9541 ggagtgcagt cccgctcgtg gataaaaatt tgtggagaaa ccaatgaatc agttgcttgt
     9601 aattgatggc gtttctgtgc gccagtactt cgaatctaac tactgtctta acgaccttca
     9661 gaaagctgct cttcttgccg ctggtgagaa tcgctcctcc cgttcgctgg aagttcacga
     9721 gtttatgcgt cgtcctgaaa cgaaggctct tgtggaatta ttggaagaag aaactacggg
     9781 agattcccgt agtattcctg tcatcaccat tcaggggcgc aatggtggga cgtatgtctg
     9841 taaagagctg gtctatgcat atgcaatgtg gatcagcccg gcattcagct taaaagtgat
     9901 acgtactttt gatgcgcttc ataattcatc accagaagaa accacatccg acaaaattaa
     9961 atccggggtc attctgcttg aatcagcagc aaagactcta aatctgtcaa actcctcgaa
    10021 acttggtgca taccagaaat tatcaaaggt agctggtctt cctgaactta tgccgatcta
    10081 tgccattgat gcacctgctg atgcgccaga tggttcaagc cgccctacgc tgtcgctgag
    10141 tgcactgctg aagcagtatg gtatccgcct gacggctaat caggcatatc accagatggc
    10201 gaagctgggg atcgttgaac aacgcgaacg atacagccct accgcgatta acaacatcaa
    10261 aaaattctgg tcgctgaccg cgaaaggctg catgttcggc aagaacatca ccagtcctgc
    10321 aaatccgcgc gagacgcagc cgcatttctt cgaatcccga ttccctgagc tgttaaagct
    10381 gctcgatacc gttcattgag gtgaccgtga gagcactact gacccctgaa atcgccccgc
    10441 gtatggggat cgtattgttc aggccaggtt cagagctgat gcccctgttt atgcaggggc
    10501 gtgtcctgct ggagcctgag ccggagcgtt attcatcttt cgccagtggt gccgttccgg
    10561 cggcatcaca accgctggcg gatgatcctg ccgttcgggc cgtgttccgc aatgaggcag
    10621 tgatccgtcg tgctggtggc gtggaatgtc ttgaaagctg gttacttcgt gaaaaaggct
    10681 gccagtggcc tcattccgac tggcacagcg agaacatgac cacaatgcga cacgctccgg
    10741 gcgcaatccg tctgtgctgg cactgcgata accagctgcg cgatcagttc acggaacggc
    10801 tggaatcaat ggcgacggat aactgtgccc gctgggtgtt gtctgttgtg cgtcgggatc
    10861 tcggttttga tgatagtcac gttgtgacaa tgccggaact gtgctggtgg ctgattcgta
    10921 atgacttggc ggatgcctta ccggaaagtg cagcccgtaa ggcactgaga ttaccaaatc
    10981 ttgttgtgcc gtctgtcacc cgggaaagtg accttgtgcc ttcggttcct gccaccagca
    11041 tcatccagga taaggcaaaa aaggtgctgg cgctgaaagt ggatccggag tcgccggagt
    11101 cttttatgtt acgcccaaaa cgtcgccgct gggttaatga aaagtacacg cgctgggtta
    11161 agacacagcc gtgtgcatgt tgtggaaagc ccgctgatga tccccaccac ctgataggtc
    11221 acggtcaggg tggaatggga acaaaagcgc atgacctctt tgtgttgcct ttgtgtagaa
    11281 agcatcacga cgagctgcat gcggataccg tggcattcga agagaagtat ggctcccagc
    11341 tggagctgat atttcgtttt atcgatcgtg cgctggcaat aggcgtactg gcgtaagtgg
    11401 agaacgagca tgaaccttga agccttacca aaatattact ccccaaaatc tccaaaattg
    11461 agcgatgacg caccggcgac aggctcgggt ggtttaacga ttacagatgt gatggctgcg
    11521 caggggatgg tgcagtcgaa agcaccgctt gggtttgcct tattcctggc aaaagttggt
    11581 gttcaggatc ctcagtttgc gattgaaggt ctgctcaatt acgcgatggc actggataac
    11641 ccgacattga ataaattgag tgaagaaacc cggttacaga tcatccctta ccttgtgaat
    11701 tttgcctttg ctgattattc ccggtctgcg gaaagtaagg ctcgctgtga gcgttgtgct
    11761 ggtactggat ttcataatgt attgcgcgaa gtggtgaaac actccagaag cggggaatct
    11821 gttatcaagg aagagtgggt gaaggaacta tgtcagcatt gccatggtaa gggagaagtc
    11881 agcacagcgt gcagagggtg taagggtaaa ggtattgtcc tggatgaaaa aagaacccgg
    11941 cttcatggcg cgcctgttta taagatttgt gggcgttgca atggaaaccg gtttagccgt
    12001 ttaccaacca cactggcgag gcatcgtgtc cagaagctga taccagacct gacggattat
    12061 cagtggtaca aaggatatgc agatgtcatt gataaactgg ttacaaagtg ctggcaggaa
    12121 gaagcgtatg ctgaagagca attgagaaaa gtgacaagat agataatttc gccgaagatg
    12181 tcgacatgat gcttgcattt ttcaaaaaat atggataaag ttttcccaac gatgggcttt
    12241 gtatgtctac cgttgataag atttaagagc ccgccgctga gcgggttttt gttgcatctc
    12301 ctgttaaata ccgccattag ctcatcagga tagagcgcca gccttcgaag ctggttgcgc
    12361 ggggttcgag tcctcgatgg cggtccatta tcggtacact gcgttgttag ctcagccgga
    12421 cagagcaatt gccttctaag caatcggtca ctggttcgaa tccagtacaa cgcgccataa
    12481 ttatttacca ggctcgcttt tgcgggcctt ttttatatct gcgccgggtc tggtatcgta
    12541 ttacttcagc caaaaggaat acctgtatat gaagtgtata ttgttaaaat gggtactgtg
    12601 cctgttactg ggcttttctt cggtatccta ttcccgggaa tttacgatag acttttcgac
    12661 tcaacaaagt tatgtctctt cgttaaatag tatacggaca gaaatatcga ccccacttga
    12721 acatatatct caggggacca catcggtatc tgttattaac cacaccccac cgggcagtta
    12781 ttttgctgtg gatatacgag ggcttgatgt ctatcaggcg cgttttgacc atcttcgtct
    12841 gattattgag caaaataatt tatatgtggc cggattcgtt aatacggcaa caaatacttt
    12901 ctacagattt tcagattttg cacatatatc agtgcccggt gtgacaactg tttccatgac
    12961 aacggacagc agttatacca ctctgcaacg tgtcgcagcg ctggaacgtt ccggaatgca
    13021 aatcagtcgt cactcactgg tttcatcata tctggcgtta atggagttta gtggaaatgc
    13081 catgaccaga gatgcatcca gagcagttct gcgttttgtc actgtcacag cagaagcctt
    13141 acggttcagg caaatacaga gagaatttcg tctggcactg tctgaaactg ctcctgttta
    13201 tacgatgaca ccggaagaag tggacctcac actgaactgg gggagaatca gcaatgtgct
    13261 tccggagttt cggggagagg gtggtgtcag agtggggcga atatccttta ataatatatc
    13321 agcgatactg ggcacagtgg cggttatact gaattgccat catcaggggg cacgttccgt
    13381 tcgctccgtg aatgaagaga tacaaccaga atgtcagata actggcgaca ggccagttat
    13441 aaggctaaac aatactttat gggaaagtaa taccgcagct gcttttctga atcgcagggc
    13501 tcactcttta aatacatccg gagaataacg ggagttaaat atgaagaaga tatttgtagc
    13561 ggctttattt gcttttgttt ctgttaatgc aatggcagct gattgtgcaa aaggtaaaat
    13621 tgagttctct aagtataatg agaatgatac attcacagta aaagtggccg ggaaagagta
    13681 ctggactaac cgctggaatc tgcaaccgct actgcaaagc gcacagttaa caggaatgac
    13741 ggtaacaatc aaatcaaata cctgtgcgtc aggttcagga tttgctgaag tgcagtttaa
    13801 ttaatatcag aagcattgct ggtttcgtag tgtgcagcaa tgtagttaca gtgtaatcaa
    13861 tgtcacaatt cagtcagttg acggttgcct gtctggctga gcatttgtta aaaaaatcct
    13921 gcatgatgaa tccccctgtg cggaggggca taatgacaga tgtttggttg cgtattgtat
    13981 aggcaagttg cggattctgt ctggtcattg cagaattcac cgggaggcac ccggcatcat
    14041 gctgtataca gagattaggc atatatccag gctcctcatc gcaggagcct ttttacatgc
    14101 aaaaaaagcc cgcgccggga gacgcgggcg gcaaggaata aacaacaaaa cgtgaagtaa
    14161 tatttcagct ggcgaataat acccgacagt aatcactctg cgcaactgcg cggccttttt
    14221 cgtattgcgg gctgtagtct tccttctgtc attgtccttt aacttccgga cctcagcccg
    14281 ctctttatct gattcagtac actatcccgg ccgggaggat tcatgacatt taaacactat
    14341 gacgtggtca gggcggcgtc gccgtcagac cttgcggatg cgcttgctca aaaaattcgt
    14401 gaaggatggc agccatacgg cgggccgttt tcttcgtata cggatgatgg cgcagcactt
    14461 attcaggcga ttgtcgcaga aggtgatgtg agcacacctg tgttggtgaa gccgtcggat
    14521 ggagaaggca cagttatcag caccaccagc gaaccggagt attactttgt tgtggttctg
    14581 gcggggcagt caaacagcat ggcatatggt gaaggccttc cgctgccgga gacatatgac
    14641 cgtccggacc cgcgtattaa gcagctggcg cgccgcagta cggtgacacc gggcggtgcc
    14701 gcctgtaagt ataacgacat tattccggcg gaccattgtc tgcatgatgt gcaggacatg
    14761 agccgtctta accatccgaa agcggacctg tcaaaggggc agtacggaac cgtggggcag
    14821 gggctgcata tcgccaaaaa actgctgccg tttataccgg cgaatgcggg catt
//
LOCUS       DAZGSP010000121        14718 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZGSP010000121 DAZGSP010000000
VERSION     DAZGSP010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.122"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            493..708
                     /gene="essD"
                     /locus_tag="ACHK74_004255"
     CDS             493..708
                     /gene="essD"
                     /locus_tag="ACHK74_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFP0222969.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            708..1205
                     /gene="rrrD"
                     /locus_tag="ACHK74_004256"
     CDS             708..1205
                     /gene="rrrD"
                     /locus_tag="ACHK74_004256"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFP0222970.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETMRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            1422..1604
                     /gene="rzoD"
                     /locus_tag="ACHK74_004257"
     CDS             1422..1604
                     /gene="rzoD"
                     /locus_tag="ACHK74_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFP0222971.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1695..1988)
                     /gene="iss"
                     /locus_tag="ACHK74_004258"
     CDS             complement(1695..1988)
                     /gene="iss"
                     /locus_tag="ACHK74_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFP0222972.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGFLGFITLGIYTPLEARVYCSQ"
     gene            complement(2348..2542)
                     /locus_tag="ACHK74_004259"
     CDS             complement(2348..2542)
                     /locus_tag="ACHK74_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0222973.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
     gene            2931..3476
                     /locus_tag="ACHK74_004260"
     CDS             2931..3476
                     /locus_tag="ACHK74_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFP0222974.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            3451..5376
                     /locus_tag="ACHK74_004261"
     CDS             3451..5376
                     /locus_tag="ACHK74_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP0222975.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQRDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            5373..5579
                     /locus_tag="ACHK74_004262"
     CDS             5373..5579
                     /locus_tag="ACHK74_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFP0222976.1"
                     /translation="MTRQEELAAARAALHDLMTGKWVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            5576..7177
                     /locus_tag="ACHK74_004263"
     CDS             5576..7177
                     /locus_tag="ACHK74_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0222977.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            7158..8477
                     /locus_tag="ACHK74_004264"
     CDS             7158..8477
                     /locus_tag="ACHK74_004264"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP0222978.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            8487..8819
                     /locus_tag="ACHK74_004265"
     CDS             8487..8819
                     /locus_tag="ACHK74_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP0222979.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            8875..9900
                     /locus_tag="ACHK74_004266"
     CDS             8875..9900
                     /locus_tag="ACHK74_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP0222980.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFEPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVEKDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            9940..10338
                     /locus_tag="ACHK74_004267"
     CDS             9940..10338
                     /locus_tag="ACHK74_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP0222981.1"
                     /translation="MTKDELIARLKLLGEQLNRDVSLTGTKEELALRVAELEEELDDS
                     DDTSGQDTPVSPENALTGHENEVVSEPPDTVIQDTADLVTVVALVTLHTDALHATRDE
                     PVDFVLPGTEFRVSAGVAVEMTERGLARMQ"
     gene            10350..10703
                     /locus_tag="ACHK74_004268"
     CDS             10350..10703
                     /locus_tag="ACHK74_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP0222982.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            10715..11293
                     /locus_tag="ACHK74_004269"
     CDS             10715..11293
                     /locus_tag="ACHK74_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0222983.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISLSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARIVLSRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            11290..11685
                     /locus_tag="ACHK74_004270"
     CDS             11290..11685
                     /locus_tag="ACHK74_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP0222984.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            11693..12433
                     /locus_tag="ACHK74_004271"
     CDS             11693..12433
                     /locus_tag="ACHK74_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0222985.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGNGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASASVVKGQSTTLTVAFQPEGVTDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSSNGEFAAVAEITVTAS"
     gene            12449..12871
                     /locus_tag="ACHK74_004272"
     CDS             12449..12871
                     /locus_tag="ACHK74_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP0222986.1"
                     /translation="MFLKTESFEYNGVTVTLSELSALQRIEHLAWLKEQEKKAESSGN
                     LQVSVEDLIRGGAFLVAMSLWHNHPQKTKLPSMNEAITQIEQEVLTTWPTEAIAQAEN
                     VVLRLSGMSEFVVNNAPEQADDAGPTEPVSAGKCSTVS"
     gene            12853..13287
                     /locus_tag="ACHK74_004273"
     CDS             12853..13287
                     /locus_tag="ACHK74_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP0222987.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPTSPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            13280..>14718
                     /locus_tag="ACHK74_004274"
     CDS             13280..>14718
                     /locus_tag="ACHK74_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP0222988.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQS
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWSLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQA"
BASE COUNT         3460 a         3633 c         4370 g         3255 t
ORIGIN      
        1 gtttctcatt taatttgtga agtagatctc tatttttatc tgaacttttt ctatcgaaac
       61 ctatttatgg ctcttatttg aacaaaaata aacctattag ctaatttata ttaatggctg
      121 ttatttatgg ggggggtata attcggtggt ttaatttaaa ttaactaaaa ataacgccgg
      181 aaattattta ttggttattt gttgaggttt tcttatgtat ttgtggtggt gttttgaaca
      241 ctcggtagca ttctcataaa tatcattcag tggtttacgt acgtaaaaaa ttggttatgc
      301 tgttaagagt ggttacttcg tcacacagtt taaacccgcc gtcgagctgg tttttccatt
      361 ttttgagtct cgatattagc tgataactca atacctgagt tattcactga ctccgagtct
      421 gttacgtttc tgcttttttg cgatacgttg tattccctca atttacaccc gctttgtctg
      481 cgaggtgggg ttatgaaatc catggataag ttaacaacgg gtgtcgccta tggcacctca
      541 gcaggtagtg ccggttactg gtttttacag ctgctcgata aagtcacgcc ctcacagtgg
      601 gcagcaatag gtgtgctggg tagcctggta tttggcctgc tgacgtacct gacaaacctt
      661 tatttcaaga ttaaagaaga taagcgcaag gctgcgagag gtgaataatg cctccatcat
      721 tacgaaaagc cgttgctgct gctattggtg gcggagcaat tgctatagca tcagtgttaa
      781 tcactggccc aagtggtaac gatggtctgg aaggtgtcag ctacatacca tacaaagata
      841 ttgttggtgt atggactgta tgtcacgggc atacaggaaa agacatcatg ctcggtaaaa
      901 cgtataccaa agcagaatgc aaagccctcc tgaataaaga ccttgccacg gtcgccagac
      961 aaattaaccc gtacatcaaa gtcgatatac cggaaacaat gcgcggcgct ctttactcat
     1021 tcgtttacaa cgtgggtgct ggcaatttca gaacatcgac gcttcttcgc aaaataaacc
     1081 agggcgatat caaaggcgca tgtgatcagc tacgtcgctg gacatatgct ggcggtaagc
     1141 aatggaaagg tctcatgact cgtcgtgaga ttgagcgtga aatctgtttg tggggtcagc
     1201 aatgaacaga gtaaccgcga ttatctccgc tctggttatc tgcatcatcg tctgcctgtc
     1261 atgggctgtt aatcattacc gtgataacgc cattacctac aaagcccagc gcgacaaaaa
     1321 tgccagagaa ctgaagctgg cgaacgcggc aattactgac atgcagatgc gtcagcgtga
     1381 tgttgctgcg ctcgatgcaa aatacacgaa ggagttagct gatgcgaaag ctgaaaatga
     1441 tgctctgcgt gatgatgttg ccgctggtcg tcgtcggttg cacatcaaag cagtctgtca
     1501 gtcagtgcgt gaagccacca ccgcctccgg cgtggataat gcagcctccc cccgactggc
     1561 agacaccgct gaacgggatt atttcaccct cagagagagg ctgatcacta tgcaaaaaca
     1621 actggaagga acccagaagt atattaatga gcagtgcaga tagagctgcc catatcgatg
     1681 ggcaactcat gcaattattg tgagcaatac acacgcgctt ccagcggagt ataaatgcct
     1741 aaagtaataa aaccgagaaa tccatttacg aatgtttgct gggtttctgt tttaacaaca
     1801 ttttctgcgc cgccacaaat tttggctgca tcaacagttt tctcctgtcc aattcccgaa
     1861 acgaagaaat gatgggtgat ggtttccttt ggtgttactg ctgtcggttt gtttccaaca
     1921 gtaaacgtct gttgagcaca tcctgtaata agcattgcca gagcggcaga aaacaacatt
     1981 tttttcatct tattatcctg cattgttaaa aacggcagaa tcctatgtga caacaattaa
     2041 acgatagtta aatggattga tgaaaattaa aactatatag gtggatgctc agcctattgg
     2101 aggagggggg cactcagaat cctgtggaat gaaataaacc gctctatctg tccattaccc
     2161 ttttagctgc gctgtatcgt cgccgtattc ccgcattaac catgaccgta gcccgacggg
     2221 gaattccttc tgcgtgagtg tgcgggaata atcaaaaacg atgcacaccg ggttttactg
     2281 tgctgacaga cgcagggtta ccctcatagt cgcttttccg gtgcgatggt ggaagaaacc
     2341 gggatgttca tccatcatca ctttggattg atgtatatgc tctcttttct gacgttagtc
     2401 tccgacggca ggcttcaatg acccaggctg agaaattccc agaccctttt tgctcaagag
     2461 cgatgttaat ttgttcaatc atttggttag gaaagcggat gttgcgggtt gttgttctgc
     2521 gggttctgtt cttcgttgac atgaggttgc cccgtattca gtgtcgctga tttgtattgt
     2581 ctgaagttgt ttttacgtta agttgatgca gatcaattaa tacgatacct gcgtcataat
     2641 tgattatttg acgtggtttg atggcgtaga tgcacgttgt gacatgtaga tgataattat
     2701 tatcattttg cgggtccttt ccggcgatcc gacaggttac ggggcggcga cctcgcgggt
     2761 tttcgctatt tatgaaaatt ttccggttta aggcgtttcc gttcttcttc gtcataactt
     2821 aatgttttta tttaaaatac cctctgaaaa gaaaggaaac gacaggtgct gaaagcgagc
     2881 tttttggcct ctgtcgtttc ctttctctgt ttttgtccgt ggaatgaaca atggaagtca
     2941 acaaaaagca gctggctgac attttcggtg cgagtatccg taccattcag aactggcagg
     3001 aacagggaat gcccgttctg cgaggcggtg gcaagggtaa tgaggtgctt tatgactctg
     3061 ccgccgtcat aaaatggtat gccgaaaggg atgctgaaat tgagaacgaa aagctacgcc
     3121 gggaagttga agaactgcgg caggctagcg agacagatct ccagccaggg actattgagt
     3181 acgaacgcca tcgacttacg cgtgcgcagg ccgacgcaca ggagctgaaa aatgccagag
     3241 actccgctga agtggtggaa accgcattct gtactttcgt gctgtcgcgg atcgcaggtg
     3301 aaattgccag tattctcgac gggatccccc tgtcggtgca gcggcgtttt ccggaactgg
     3361 aaaaccgaca tgttgatttc ctgaaacggg atatcatcaa agccatgaac aaagcagccg
     3421 cgctggatga actgataccg gggttgctga gtgaatatat cgaacagtca ggttaacagg
     3481 ctgcggcatt ttgtccgcgc cgggcttcgc tcactgttca ggccggagcc acagaccgcc
     3541 gttgaatggg cggatgccaa ttactatctc ccaaaagaat ccgcatacca ggaagggcgc
     3601 tgggaaacac tgccctttca gcgggccatc atgaatgcga tgggcagcga ctacatccgt
     3661 gaggtgaatg tggtgaagtc tgcccgtgtc ggttattcca aaatgctgct gggtgtttat
     3721 gcctacttta tagagcataa gcagcgcaac acccttatct ggttgccgac ggatggtgat
     3781 gccgagaact ttatgaaaac ccacgttgag ccgaccatcc gcgatattcc gtcgctgctg
     3841 gcgctggctc cgtggtatgg caaaaagcac cgggataaca cgctcactat gaagcgtttt
     3901 tccaatggtc gtggcttctg gtgcctgggc ggtaaagcgg caaaaaacta ccgtgaaaag
     3961 tcggtggatg tggcgggtta tgatgaactt gctgcctttg atgaggatat tgaacaggaa
     4021 ggctctccga cgttccttgg cgacaaacgt attgaaggct cggtctggcc aaagtccatc
     4081 cgtggctcca cgcccaaagt gagaggcacc tgccagattg agcgtgcagc cagtgaatcc
     4141 ccgcatttta tgcgttttca tgttgcctgc ccgcactgcg gggaggagca gtatcttaaa
     4201 tttggcgaca aagagacgcc gtttggcctc aaatggacgc cggatgaccc ctccagcgtg
     4261 ttttatctct gcgagcataa tgcctgcgtc atccgccagc aggagctgga ctttactgat
     4321 gcccgttata tctgcgaaaa gaccgggatc tggacccgtg atggcattct ctggttttcg
     4381 tcatccggtg aagagattga gccgccggac agtgtgacct ttcacatctg gacggcgtac
     4441 agcccgttca ccacctgggt gcagattgtc aaagactgga tgaagacgaa aggggatacg
     4501 ggaaaacgta aaaccttcgt gaacaccacg ctcggtgaga catgggaagc gaaaattggc
     4561 gaacgtccgg atgctgaggt gatggcggag cggaaagagc attattcagc gcccgttcct
     4621 gaccgtgtgg cttacctgac cgccggtatc gactcccagc gggaccgcta cgaaatgcgc
     4681 gtatggggat ggggaccggg tgaggaaagc tggctgattg accggcagat tattatgggc
     4741 cgccacgacg atgaacagac gctgctgcgt gtggatgagg ccatcaataa aacctatacc
     4801 cgccggaatg gtgcagaaat gtcggtatcc cgtatctgct gggatactgg cgggattgac
     4861 ccgaccattg tgtatgaacg ctcgaaaaag catgggctgt tccgggtgat ccccattaaa
     4921 ggggcatccg tctacggaaa gccagtggcc agcatgccac gtaagcgaaa caaaaacggg
     4981 gtttacctta ccgaaatcgg tacggatacc gcgaaagagc agatttataa ccgcttcaca
     5041 ctgacgccgg aaggggatga accgcttccc ggtgccgttc acttcccgaa taacccggat
     5101 atttttgatc tgaccgaagc gcagcagctg actgctgaag agcaggtcga aaaatgggtg
     5161 gatggcagga aaaaaatact gtgggacagc aaaaagcgac gcaatgaggc gctcgactgc
     5221 ttcgtttatg cgctggcggc gctgcgcatc agtatttccc gctggcagct ggatctcagt
     5281 gcactgctgg cgagcctgca ggaagaggat ggtgcagcaa ccaacaagaa aacactggca
     5341 gattacgccc gtgccttatc cggagaggat gaatgacgcg acaggaagaa cttgccgctg
     5401 cccgtgcggc actgcatgac ctgatgacag gaaaatgggt ggcaacggta cagaaagacg
     5461 gacggcgagt ggagtttacg gccacttccg tgtctgacct gaaaaaatac attgcggagc
     5521 tggaagtgca gaccggcatg acacagcgac gcaggggacc tgcaggattt tatgtatgaa
     5581 aacgtccacc attcccaccc ttctggggcc ggacggcatg acatcgctgc gtgaatatgc
     5641 cggttatcac ggcggtggca gcggatttgg tgggcagttg cgggcgtgga acccaccgag
     5701 tgaaagtgtg gatgcagccc tgctgcccaa ctttacccgt ggcaatgccc gcgcagacga
     5761 tctggtacgc aataacggct atgccgccaa cgccatccag ttgcatcagg atcatatcgt
     5821 cgggtctttt ttccggctca gtcatcgccc aagctggcgc tatctgggca tcggggagga
     5881 agaagcccgt gccttttccc gcgaggttga agcggcatgg aaagagtttg ccgaggatga
     5941 ctgctgctgc attgacgttg agcgaaaacg cacgtttacc atgatgattc gggaaggtgt
     6001 ggccatgcac gcctttaacg gtgaactgtt cgttcaggcc acctgggata ccagtccgtc
     6061 gcggcttttc cggacacagt tccggatggt cagcccgaag cgcatcagca acccgaacaa
     6121 taccggcgac agccggaact gccgtgccgg tgtgcagatt aatgacagcg gtgcggcgct
     6181 gggatattac gtcagcgagg acgggtatcc tggctggatg ccgcagaaat ggacatggat
     6241 accccgtgaa ttacccggcg ggcgcgcctc gttcattcac gtttttgaac ccgtggagga
     6301 cgggcagacc cgcggtgcaa atgtgtttta cagcgtaatg gagcagatga agatgctcga
     6361 cacgctgcag aacacgcagc tgcagagcgc cattgtgaag gcgatgtatg ccgccaccat
     6421 tgaaagtgag ctggatacgc agtcagcgat ggattttatt ctgggcgcga acagtcagga
     6481 gcagcgggaa aggctgacgg gctggattgg tgaaattgcc gcgtattacg ccgcagcacc
     6541 ggtccggctg ggaggcgcaa aagtgcctca cctgatgccg ggtgactcac tgaacctgca
     6601 gacggctcag gacacggata atggctactc cgtttttgag cagtcactgc tgcggtatat
     6661 cgctgccggg ctgggtgtct cgtatgagca gctttcccgg aattacgccc agatgagcta
     6721 ctccacggca cgggccagtg cgaacgagtc gtgggcgtac tttatggggc ggcgaaaatt
     6781 cgtcgcatcc cgtcaggcga gccagatgtt tctgtgctgg ctggaagagg ccatcgttcg
     6841 ccgcgtggtg acgttacctt caaaagcgcg cttcagtttt caggaagccc gcagtgcctg
     6901 ggggaactgt gactggatag gctccggtcg tatggccatc gatggtctga aagaagtaca
     6961 ggaagcggtg atgctgatag aagccggact gagcacctac gagaaagagt gcgcaaaacg
     7021 cggcgacgac tatcaggaaa tttttgccca gcaggtccgt gaaacgatgg agcgccgcgc
     7081 ggctggtctt aaaccgcccg cctgggcggc tgcggcattt gaatccgggc tgcgacaatc
     7141 aacagaggag gagaagagtg acagcagagc tgcgtaatct cccgcatatt gccagcatgg
     7201 cttttaatga gccgctgatg cttgaacccg cctatgcgcg ggttttcttt tgtgcgcttg
     7261 caggccagct tgggatcagc cgcctgacgg atgcagtatc cggcgacagc ctgactgccg
     7321 gagaggcacc cgcggcgctg gcgttatccg gtgatgatga cggaccacga caggcccgca
     7381 gttatcaggt catgaacggc atcgccgtgc tgccggtgtc cggtacgctg gtcagtcgga
     7441 cgcgggcgct gcagccgtat tcgggaatga ccggttacaa cggcattatc gcccgtctgc
     7501 aacaggctgc cagtgacccg atggtggacg gcattctgct cgatatggat acgccaggcg
     7561 gaatggtggc gggggcattt gactgcgctg acatcatcgc ccgtgtgcgt gacataaagc
     7621 cggtatgggc gctggccaat gacatgaact gcagcgcagg tcagctgctt gccagtgccg
     7681 cctcccggcg tctggtcacg cagaccgccc ggacaggctc catcggcgtc atgatggctc
     7741 acagtaatta cggcgctgcg ctggagaaac agggcgtgga aatcacgctg atttacagcg
     7801 gcagccataa ggtggatggc aacccctaca gccatctacc ggatgatgtc cgggaaacac
     7861 tgcagtcccg gatggatgca acccgccgga tgtttgcaca gaaggtgtcg gcatataccg
     7921 gcctgtctgt gcaggctgtg ctggataccg aggctgcagt gtacagcggt caggaggcca
     7981 ttgatgccgg actggctgat gaacttgtta acagtaccga tgcgatcacc gtcatgcgtg
     8041 atgcactgga tgcacgtaaa tcccgtctct caggagggcg aatgaccaaa gagactcaat
     8101 caacaactgt ttcagccact gcttcgcagg ctgacgttac tggcgtggtg caagcgacgg
     8161 agggcgagaa cgccagcgcg gcgcagccgg acgtaaacgc gcagatcacc gcagcggttg
     8221 cggcagaaaa cagtcgcatt atggggatcc tcaactgtga ggaggctcac ggacgtgaag
     8281 aacaggcacg cgtgctggcc gaaacccccg gtatgaccgt ggaaacggcc cgccgcattc
     8341 tggcagctgc accacagagt gcacaggcgc gcagtgacac tgcgctggat cgtctgatgc
     8401 agggggcacc ggcaccgctg gctgcaggta acccggcatc tgatgccgtt aacgatttgc
     8461 tgaacacacc agtgtaaggg atgtttatga cgagcaaaga aacctttacc cattaccagc
     8521 cgctgggcaa cagtgacccg gcacatacgg caaccgcgcc cggcggattg agtgcgaaag
     8581 cgcctgcaat gaccccgctg atgctggata cctccacccg taagctggtt gcgtgggatg
     8641 gcaccaccga cggtgctgcc gttggcattc ttgcggttgc tgctgaccag accagcacca
     8701 cgctgacgtt ctacaagtcc ggcacgttcc gttatgagga tgtgctctgg ccggaggctg
     8761 ccagcgacga gacgaaaaaa cggaccgcgt ttgccggaac ggcaatcagc atcgtttaac
     8821 tttacccttc atcactaaag gccgcctgtg cggctttttt tacgggattt ttttatgtcg
     8881 atgtacacaa ccgcccagct gctggcggca aatgagcaga aatttaagtt tgatccgctg
     8941 tttctgcgtc tctttttccg tgagagctat cccttcacca cggagaaagt ctatctctca
     9001 caaattccgg gactggtaaa catggcgctg tacgtttcgc cgattgtttc cggtgaggtt
     9061 atccgctccc gtggcggctc cacctctgag tttacgccgg gatatgtcaa gccgaagcat
     9121 gaggtgaatc cgcagatgac cctgcgtcgc ctgccggatg aagatccaca gaatctggcg
     9181 gacccggctt accgccgccg tcgcatcatc atgcagaaca tgcgagacga agagctggcc
     9241 attgctcagg tcgaagagat gcaggccgtt tctgccgtgc tcaagggcaa atacaccatg
     9301 accggtgaag ccttcgagcc ggttgaggtg gatatgggcc gcagtgcggc gaataacatc
     9361 acacagtccg gcggcacgga gtggagcaag cgtgacaagt ccacgtatga cccgaccgac
     9421 gatatcgaag cctacgcgct gaacgccagc ggtgtggtga atatcatcgt gtttgatccg
     9481 aaaggctggg cgctgttccg ttccttcaaa gccgtcaagg agaagctgga tacccgtcgc
     9541 ggctctaatt ccgagctgga gacagcggta aaagacctgg gcgaagcggt gtcctataag
     9601 gggatgtatg gcgatacggc gatcgtcgtg tattccggac agtacgtgga aaaagacgtc
     9661 aaaaagaact tcctgccgga caacacgatg gtgctgggga acactcaggc acgcggtctg
     9721 cgcacctatg gctgcattca ggatgcggac gcacagcgcg aaggcattaa cgcctctgcc
     9781 cgctacccga aaaactgggt gaccaccggc gatccggcgc gtgagttcac catgattcag
     9841 tctgcaccgc tgatgctgct ggctgaccct gatgcgttcg tgtccgtaca actggcgtaa
     9901 tcatggccct tcggggccat tttctctgtg gaggagtcca tgacgaaaga tgaactgatt
     9961 gcccgtctta agctgctggg tgagcaactg aaccgtgatg tcagcctgac ggggacgaag
    10021 gaagaactgg cgctccgtgt ggcagagctg gaagaggagc ttgatgactc ggatgacact
    10081 tccggccagg acacgcctgt cagcccggaa aatgcgctga ccgggcatga aaatgaggtg
    10141 gtatcagagc cgccggatac cgtaattcag gatacggctg atctggtcac ggtcgtggca
    10201 ctggtgacgc tgcataccga tgcacttcac gccacacggg atgagcctgt ggattttgtg
    10261 ctgccgggaa cggagttccg tgtctctgcc ggtgtggcag ttgaaatgac agagcgcggc
    10321 ctggccagaa tgcaataacg ggaggcgctg tggctgattt cgataacctg ttcgatgctg
    10381 ccattgcccg cgccgatgaa acgatacgcg ggtacatggg aacgtcagcc accatgacat
    10441 ccggtgagca gtccggcgca gtaatacgtg gtgtttttga tgaccctgaa aatatcagct
    10501 atgccggaca gggcgtgcgc gttgaaggct ccagcccgtc cctgtttgtc cggactgatg
    10561 atgtgcggca actgcggcgt ggagacacgc tgaccatcgg tgaggaaaac ttctggatag
    10621 accgggtttc gccggatgat ggtggaagct gtcatctctg gcttgggcgg ggcgtaccgc
    10681 ctgccgttaa ccgtcgccgc tgaaaggggg atgtatggcc ataaaaggtc ttgagcaggc
    10741 cgttgaaaac ctcagccgta tcagcagaac ggcggtgcct ggtgccgccg caatggccat
    10801 taaccgcgtt gcttcatccg cgatatcgct gtcggcgtca caggttgccc gtgagacaaa
    10861 ggtacgccgg aaactggtaa aggaaagggc caggctgaaa agggccacgg tcaaaaaccc
    10921 gcaggccaga atcaaggtta accgggggga tttgcccgta ataaagctgg gtaacgcgcg
    10981 gattgtcctg tcccgacgca ggcgtcgtaa aaaggggcag cgttcagccc tgaaaggtgg
    11041 cggcagcgtg cttgtggtgg gaaaccgtcg tattcccggc gcgtttattc agcaactgaa
    11101 aaatggccgg tggcatgtca tgcagcgtgt ggccgggaaa aaccgttacc ccattgatgt
    11161 ggtgaaaatc ccgatggcgg tgccgctgac cacggcgttt aaacagaata ttgagcggat
    11221 acggcgtgag cgtcttccga aagagctggg ctatgcgctg cagcatcaac tgagaatggt
    11281 aataaagcga tgaaacatac tgaactccga gcagccgtac tggatgcact ggagaagcat
    11341 gacaccgggg cgacgctttt tgatggtcgc cccgctgttt ttgatgaggc ggattttccg
    11401 gcaattgccg tttatctcac cggcgctgaa tacacgggcg aagagctgga cagcgatacc
    11461 tggcaggcgg agctgcatat tgaagttttc ctgcctgctc aggtgccgga ttcagagctg
    11521 gattcgtgga tggagtcccg gatttatccg gtgatgagcg atatcccggc actgtcagat
    11581 ttgatcacca gtatggtggc cagtggctat gactaccggc gcgacgatga tgcgggcctg
    11641 tggagttcag ccgatctgac gtatgtcatt acctatgaaa tgtgaggacg ctatgcctgt
    11701 accaaatcca acaatgccgg tgaaaggtgc cgggaccaca ctgtgggttt ataaggggaa
    11761 cggtgaccct tatgcgaacc cgctttcaga cgttgactgg tcgcgtctgg caaaagttaa
    11821 agacctgaca cccggcgaac tgaccgctga gtcctatgac gacagctatc tcgatgatga
    11881 agatgcggac tggactgcga ccgggcaggg gcagaaatct gccggagata ccagcttcac
    11941 gctggcgtgg atgcccggag agcaggggca gcaggcgctg ctggcgtggt ttaatgaagg
    12001 tgatacccgt gcctataaaa tccgcttccc gaacggcacg gtcgatgtgt tccgtggctg
    12061 ggtcagcagt atcggtaaag cggtgacggc gaaggaagtg atcacccgca cggtgaaggt
    12121 caccaatgtg ggacgcccgt cgatggctga agatcgcagt acggtgacgg cagcaaccgg
    12181 catgacggta acgccagcca gtgcttccgt agtgaaaggg cagagcacca cgctgaccgt
    12241 ggcattccag ccggaaggtg taaccgacaa gagcttccgt gcggtgtctg cggataaaac
    12301 aaaagccacc gtgtcggtca gtggtatgac catcaccgtg aacggcgttg ctgcaggcaa
    12361 ggtcaacatt ccggttgtat ccagtaatgg tgagtttgct gcggttgcag aaatcaccgt
    12421 caccgccagt taatccggag agtcagcgat gttcctgaaa accgaatcat ttgaatataa
    12481 cggcgtgacc gtcacgcttt ctgaactgtc agccctgcag cgtattgagc atctcgcctg
    12541 gttgaaagag caggaaaaaa aggctgaatc cagtggcaac ctgcaggtgt ctgtagagga
    12601 tcttatcaga ggcggggcgt ttctggtggc gatgtctctg tggcataacc atccgcagaa
    12661 gacaaagctg ccgtccatga atgaagccat tacgcagatt gagcaggaag tgcttaccac
    12721 ctggcccacg gaggcaattg ctcaggctga aaacgtggtg ttacgtctgt ccggaatgtc
    12781 tgagtttgtg gtgaataatg cccctgaaca ggcagatgac gccgggccta cagagcctgt
    12841 ttctgcggga aagtgttcga cggtgagctg agttttgccc tgaaactggc gcgtgagatg
    12901 gggcgacccg actggcgcgc catgcttgcc gggatgtcat ccacggagta tgccgactgg
    12961 caccgctttt acagtaccca ttattttcat gatgttctgc tggatatgca cttttccggg
    13021 ctgacataca ccgtgctcag cctgtttttc agcgatccgg atatgcatcc gctggatttc
    13081 agtctgctga accggcgcga ggctgacgaa gagcctgaag atgatgtgct gatgcagaaa
    13141 gcggcagggc ttgccggagg tgtccgcttt ggcccggacg ggaatgaagt tatccctact
    13201 tccccggatg tggcggacat gacggaggat gacgtaatgc tgatgacagt atcagaaggg
    13261 atcgcaggag gagtccggta tggctgaacc ggtaggcgat ctggtcgttg atttgagtct
    13321 ggatgcggcc agatttgacg agcagatggc cagagtcagg cgtcattttt ccggtacgga
    13381 aagtgatgcg aaaaaaacag cggcagtcgt tgaacagtcg ctgagccgac aggcgctggc
    13441 tgcacagaaa gcggggattt ccgtcgggca gtataaagcc gccatgcgta tgctgcctgc
    13501 acagttcacc gacgtggcca cgcagcttgc aggcgggcaa agtccgtggc tgatcctgct
    13561 gcaacagggt ggtcaggtta aggactcctt cggcgggatg atccccatgt tcagggggct
    13621 tgccggtgcg atcaccctgc cgatggtggg ggccacctcg ctggcggtgg cgaccggtgc
    13681 gctggcgtat gcctggtatc agggcaactc aaccctgtcc gatttcaaca aaacgctggt
    13741 cctttccggc aatcagtcgg ggctgacggc agatcgtatg ctggtcctgt ccagagccgg
    13801 gcaggcggca gggctgacgt ttaaccagac cagcgagtca ctcagcgcac tggttaaggc
    13861 gggggtaagc ggtgaggctc agattgcatc catcagccag agtgtggcgc gtttctcctc
    13921 tgcatccggc gtggaggtgg acaaggtcgc tgaagccttc gggaagctga ccacagaccc
    13981 gacgtcaggg ctgacagcga tggcacgcca gttccataac gtgacggcgg agcagattgc
    14041 gtatgttgct cagttgcagc gttccggcga tgaagccggg gcattgcagg cggcgaacga
    14101 ggccgcgacg aaagggtttg atgaccagac ccgccgcctg aaagagaaca tgggtacgct
    14161 ggagacctgg gcagacagga cagcgcgggc attcaaatcc atgtgggatg cggtgctgga
    14221 tattggtcgt cctgataccg cgcaggagat gctgattaag gcagaggctg cgtttaagaa
    14281 agcggacgac atctggagtc tgcgcaagga tgattatttt gttaacgatg aagcgcgggc
    14341 gcgttactgg gatgatcgtg aaaaggcccg tcttgcgctt gaagccgcca gaaagaaggc
    14401 tgaacagcag agtcaacagg acaaaaatgc gcagcagcag agcgatactg aagcgtcacg
    14461 gctgaaatat accgaagagg cgcagaaggc ttacgaacgg ctgcagacgc cgctggagaa
    14521 atataccgcc cgtcaggaag aactgaacaa ggcactgaag gacgggaaaa ttctgcaggc
    14581 agattacaac acgctgatgg cggcggcgaa aaaggactat gaagcgacgc tgaaaaagcc
    14641 gaaacagtcc ggcgtgaagg tgtctgcggg cgatcgtcag gaagacagtg ctcatgctgc
    14701 cctgctgacg cttcaggc
//
LOCUS       DAZGSP010000122        14685 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZGSP010000122 DAZGSP010000000
VERSION     DAZGSP010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.123"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            47..1198
                     /gene="yiaY"
                     /locus_tag="ACHK74_004275"
     CDS             47..1198
                     /gene="yiaY"
                     /locus_tag="ACHK74_004275"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFP0222989.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1363..2901
                     /gene="aldB"
                     /locus_tag="ACHK74_004276"
     CDS             1363..2901
                     /gene="aldB"
                     /locus_tag="ACHK74_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFP0222990.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            3179..3363
                     /locus_tag="ACHK74_004277"
                     /pseudo
     CDS             3179..3363
                     /locus_tag="ACHK74_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833473.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            3380..3790
                     /locus_tag="ACHK74_004278"
     CDS             3380..3790
                     /locus_tag="ACHK74_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP0222991.1"
                     /translation="MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALV
                     AHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWLLVDINMNQFEGRAERINITMPK
                     RLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGA"
     gene            complement(3862..5826)
                     /locus_tag="ACHK74_004279"
     CDS             complement(3862..5826)
                     /locus_tag="ACHK74_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFP0222992.1"
                     /translation="MNISEVDLRKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDRVFGPDEDKLQGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFTSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPADAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEETSA"
     gene            complement(5837..7237)
                     /locus_tag="ACHK74_004280"
     CDS             complement(5837..7237)
                     /locus_tag="ACHK74_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0222993.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMEILLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            7463..8278
                     /locus_tag="ACHK74_004281"
     CDS             7463..8278
                     /locus_tag="ACHK74_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0222994.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVADPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADAAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            complement(8310..9005)
                     /gene="araD"
                     /locus_tag="ACHK74_004282"
     CDS             complement(8310..9005)
                     /gene="araD"
                     /locus_tag="ACHK74_004282"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP0222995.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIKTFEERGLNP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGDNAYYGQ"
     gene            complement(8999..9859)
                     /locus_tag="ACHK74_004283"
     CDS             complement(8999..9859)
                     /locus_tag="ACHK74_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFP0222996.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMIETGVAIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTQQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(9852..10514)
                     /gene="ulaD"
                     /locus_tag="ACHK74_004284"
     CDS             complement(9852..10514)
                     /gene="ulaD"
                     /locus_tag="ACHK74_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP0222997.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGL
                     SAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHTMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQARGQQWGEADLALMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGA
                     RA"
     gene            complement(10511..12007)
                     /gene="lyxK"
                     /locus_tag="ACHK74_004285"
     CDS             complement(10511..12007)
                     /gene="lyxK"
                     /locus_tag="ACHK74_004285"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFP0222998.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAALTGLKAGTSVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKVG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHTL"
     gene            complement(12011..12997)
                     /gene="yiaO"
                     /locus_tag="ACHK74_004286"
     CDS             complement(12011..12997)
                     /gene="yiaO"
                     /locus_tag="ACHK74_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFP0222999.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPHGADLLKAVDEVQ"
     gene            complement(13010..14287)
                     /gene="yiaN"
                     /locus_tag="ACHK74_004287"
     CDS             complement(13010..14287)
                     /gene="yiaN"
                     /locus_tag="ACHK74_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFP0223000.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            complement(14290..>14685)
                     /gene="yiaM"
                     /locus_tag="ACHK74_004288"
     CDS             complement(14290..>14685)
                     /gene="yiaM"
                     /locus_tag="ACHK74_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFP0223001.1"
                     /translation="RYGFQTSILSVDELSRYLFVWLTFIGAIVAFMDNAHVQVTFLVE
                     KLSPAWQRRVALVTHSLILFICGALAWGATLKTIQDWSDYSPILGLPIGLMYAACLPT
                     SLVIAFFELRHLYQLITRSNSLTSPPQGA"
BASE COUNT         3521 a         3905 c         3937 g         3322 t
ORIGIN      
        1 tcctcagtaa gctgcccacc cttttttaca ctttcaggag tgtgttatgg catcttcaac
       61 tttctttatt ccttctgtga atgtcatcgg cgctgattca ttgactgatg caatgaatat
      121 gatggcagat tatggattta cccgtacctt aattgtcact gacagtatgt taacgaaatt
      181 aggtatggcg ggcgatgtgc aaaaagcact ggaagaacgc aatattttta gcgttattta
      241 tgatggcacc caacctaacc ccaccacgga aaacgtcgcc gcaggtttga aattacttaa
      301 agagaataat tgcgatagcg tgatctcctt aggcggtggt tctccacacg actgcgcaaa
      361 aggtattgcg ctggtggcag ccaatggcgg cgatattcgc gattacgaag gcgttgaccg
      421 ctctgcaaaa ccgcagctgc cgatgatcgc catcaatacc acggcgggta cggcctctga
      481 aatgacccgt ttctgcatca tcactgacga agcgcgtcat atcaaaatgg cgattgttga
      541 taaacatgtc actccgctgc tttctgtcaa tgactcctct ctgatgattg gtatgccgaa
      601 gtcactgacc gccgcaacgg gtatggatgc cttaacgcac gctatcgaag cgtatgtttc
      661 tattgccgcc acgccgatca ctgacgcttg tgcactgaaa gccgtgacca tgattgccga
      721 aaacctgccg ttagccgttg aagatggcag taatgcgaaa gcgcgtgaag caatggctta
      781 tgcccagttc ctcgccggta tggcgttcaa taatgcttct ctgggttatg ttcatgcgat
      841 ggcgcaccag ctgggcggtt tctacaacct gccacacggt gtatgtaacg ccgttttgct
      901 gccgcatgtt caggtattca acagcaaagt cgccgccgca cgtctgcgtg actgtgccgc
      961 tgcaatgggc gtgaacgtga caggtaaaaa cgatgcggaa ggtgctgaag cctgcattaa
     1021 cgccatccgt gaactggcga agaaagtgga tatcccggca ggcctacgcg acctgaacgt
     1081 gaaagaagaa gatttcgcgg ttctggcgac taatgccctg aaagatgcct gtggttttac
     1141 taacccgatc caggcaactc acgaagaaat tgtggcgatt tatcgcgcag cgatgtaatc
     1201 atcatttcca caacggctgg caaattgtca gccgcttttt caactatctc tgtaaccctt
     1261 gcccgtaaat tcgtgatagc tgtcgtaaag ctgttaccga ctggcgaaga tttcgccagt
     1321 cacgtctacc cttgttatac ctcacaccgc aaggagacga tcatgaccaa taatccccct
     1381 tcagcacaga ttaagcccgg cgagtatggt ttccccctca agttaaaagc ccgctatgac
     1441 aactttattg gcggcgaatg ggtagcccct gccgacggcg agtattacca gaatctgacg
     1501 ccggtgaccg ggcagctgct gtgcgaagtg gcgtcttcgg gcaaacgaga catcgatctg
     1561 gcgctggatg ctgcgcacaa agtgaaagat aaatgggcgc acacctcggt gcaggatcgc
     1621 gcggcgattc tgtttaagat tgccgatcga atggaacaaa acctcgagct gttagcgaca
     1681 gctgaaacct gggataacgg caaacccatt cgcgaaacca gtgctgcgga tgtaccgctg
     1741 gcgattgacc atttccgcta tttcgcctcg tgtattcggg cgcaggaagg tgggatcagt
     1801 gaagttgata gcgaaaccgt ggcctatcat ttccatgaac cgttaggcgt ggtggggcag
     1861 attatcccgt ggaacttccc gctgctgatg gcgagctgga aaatggctcc cgcgctggcg
     1921 gcgggcaact gtgtggtgct gaaacccgca cgtcttaccc cgctttctgt actgctgcta
     1981 atggaaattg tcggtgattt actgccgccg ggcgtggtga acgtggtcaa tggcgcaggt
     2041 ggggaaattg gcgaatatct ggcgacctcg aaacgcatcg ccaaagtggc gtttaccggc
     2101 tcaacggaag tgggccaaca aattatgcaa tacgccacgc aaaacattat tccggtgacg
     2161 ctggagctgg gcggcaaatc gccaaatatc ttctttgctg acgtgatgga tgaagaagat
     2221 gcctttttcg ataaagcgct ggaaggcttt gcactgtttg cctttaacca gggcgaagtt
     2281 tgcacctgtc caagccgtgc tttagtgcag gaatctatct acgaacgctt tatggaacgc
     2341 gccatccgcc gtgtcgaaag cattcgtagc ggtaacccgc tcgacagcgt gacgcaaatg
     2401 ggtgcgcagg tttctcacgg gcaactggaa accatcctca actacattga tatcggtaaa
     2461 aaagagggcg cagacgtgct caccggcggg cggcgcaagc tgctggaagg tgaactgaaa
     2521 gacggctact acctcgaacc gacgattctg tttggtcaga acaatatgcg cgtgttccag
     2581 gaagaaattt ttggcccggt gctggcggtg accaccttca aaacgatgga agaagcgctg
     2641 gagctggcga acgacacgca atatggcctg ggcgcaggtg tctggagccg caacggtaat
     2701 ctggcctata agatggggcg cggcattcag gccgggcgcg tgtggaccaa ctgctatcac
     2761 gcttacccgg cacatgcggc gtttggtggc tacaaacaat caggcatcgg tcgtgaaacc
     2821 cacaaaatga tgcttgagca ttaccagcaa accaagtgcc tgctggtgag ctactcggat
     2881 aaaccgttgg ggctgttctg atataagaag ctggtcgcat tgggtattca ttgcctgatg
     2941 cgacgtttgc gcattttacc aattcaaatg tctacgtctt gaatgactga atattgattg
     3001 aacttaatta cgagtctcct cgcatctttt caacaagtta ctgcacaccg ttattctcct
     3061 tccgagcggc attcctgagt caactttatc cccctgacgg cccatataaa tcgcgctatc
     3121 gtgattgtta ttgctaattg tgtgtgtatc gtgtgtataa tcatggaagg gcagggatgt
     3181 gaaaagtgca gacgtgatcg ccatcctgaa gcagcatggc tgggaacata ttcgaacccg
     3241 tggcagtcat catcagttcc gccaccctgt tcatccgggt ctggtgacgg ttccgcaccc
     3301 taaaaagata ttaagcccgg tacgctggcg caaattgggc gtcaggctgg tttaacattt
     3361 taaaatttca ggaggcaata tgttttatcc cgcctatatc cacagtgatc ctgacggcag
     3421 cgccagcggt tttttccctg atgttccagg ctgttatttt gctggtgata cgctggacaa
     3481 cgctttccag gatgcgcgaa gtgcactggt cgcgcacttc gaaaccttat gcgagatgaa
     3541 tgaagagttg ccgcttccag gctctgtgga aacacatctg gtccagcgtg cgcaggactt
     3601 tattggcggg caatggttgc tggtggatat caatatgaat cagttcgaag gtcgggctga
     3661 acgtatcaat attacgatgc caaaaagatt actgaacaag attgatacct acgtgcgcaa
     3721 taacccagac tacgccaaca gaagtgcgtt tctggcggaa gctgcccggc gtgtattgcc
     3781 gggcgcttaa tcccgcgttt cgatggttgg cgggcaaccg caccgatatg ttgatactga
     3841 taagacgcgt taagcgtcgc atcatgcgct ggtttcctca ttcacccaga tccgcatttc
     3901 gccttcaccc cggttggccc agctaaacca cgggataaat gtcagagttt gcgtctggcg
     3961 tttggctggc gcgctgtcgt aatgccacag cggttgctgc tctggattgc tctgttcata
     4021 ccggtaaccc ggagcctgga ttaagatctt atggctaaac aatcccttgc cttcaaatgt
     4081 cgtaaatggc gcatcggcgg gcagccacag attatgcagt gactcgccgt tgtcggcctg
     4141 ttccaggcaa tacaccagcg gaccgcgctg aatcgccact tttccggcga cgtgacgcac
     4201 cagcgggtta ccgtaaacgc gacgtaccgg cattggcaaa gtcagattca gcgtgtcgcc
     4261 ctcctgccat tcgcgggtaa tgtgcaaata ccctttacga atatcctgct cgacctcttc
     4321 cccattcaat atgatctgcg gctgtgtgca ccagtccggc agacgtaaag ccagcgtatg
     4381 acgtaccggc tggggcgatt caaccgcaat cgtcacctgc tcctgccacg gatagttccc
     4441 gctaacccgc aggcgcagcg tgccattttc taccggcact tccatgctgt ttcctgcgta
     4501 tatgttgata tacaacgcat cttcacgcgg cgtgtagaga taatgaccaa tcgaggtcag
     4561 cacgcgggcg atatttggcg gacaacaagc gcaaccaaac caacgctggc ggatcggttt
     4621 aacgtgatcg tagatatggt tgaatttcag cgattttgga tgtacttcca gcggattcac
     4681 atagaagaaa tgtttgccat ccagcgccat gccgccgagc acggtgttgt acagcgcgcg
     4741 ctccatcaca tcggcatatt gactgtcgcc ttccatttcc agcattcgcc gggcgaacat
     4801 catcaggccg atggaagcac aactttcggc gtagaccgtg tcattcggca gatcgtaatc
     4861 gctagtgaac gcttcgccgc tgctttgcga gccaatgccg ccggtaatat ataactgacg
     4921 ctgggccata ttgttccaca gcctcaggca gtcctgacgc ttgctgtcat cgtgacttaa
     4981 acgcgcgaga tgcgcgacgc cggtcatcag gtagacaaaa cgtaccgcgt gaccgatggc
     5041 ggtttgctgt tgcgcaaggg gcaaatgtgc ctggctgtag gctttgtctt tcaccatcca
     5101 cgccgggccg taggtgtgcc agtgcgaggt ctgcccgcgc ttttcatatt cctggtcgta
     5161 atagtgcggt tgcgcaccac gctgttcgac aaaatagttc gtcagcgcca ggtagcgcgg
     5221 ctcttcggtg acttcgtaga gacgcatcag cgccagttca atttccggat gaccgggata
     5281 accctgcagc ttgtcttcat ctggaccaaa tacgcggtcg atatgatcgg ccagacggca
     5341 aaccacctcc agcaagcgtc gttttcccgt ggcctggaag aaggcgactc cggcttcaat
     5401 cagatgacct gcgcagtaaa gttcatgaca ctccgccaga ttgctccagc gttcttcggg
     5461 tgcttttacc gtaaagtaag tattgagata gccgtcttcg cattgggcgg aggcgatcag
     5521 ttcgattacc tcgtcggcgg ttttttccag ttcggcgtcc ggcttctggc acagcgacca
     5581 ggctaccgct tccagccatt tggcgacgtc gctgtcctga aacaccatcc cgtaaaattc
     5641 accctcctga agtccggcag caatgcgaaa gttttcaatc gcatggctgg gttccgcttc
     5701 tgggatacga tcgttcaagg catcccactg ataaggaatc accacgtcgc ggaccagttg
     5761 ttggtactga ccgaggaacg gatcgctgac cgtcagttta cgcagatcga cttccgaaat
     5821 gttcatcttg tgctccttaa ggctgtacat gacgctgacg caggtcggta gctatgcgtg
     5881 acatcatagg attattgagt ttgcaaaagc ggatcgcccc tgccagcagc aggtggaata
     5941 gcgcaggcag taatatttcc atcgccgcga tcccgccaag cgaactggcg gtctggtttt
     6001 ccacgccagg ctggtaggcg acaaaaataa acagcaggct gatgatcccg gcgctggagg
     6061 cccaggccag cttgatgaaa aacagattga aggcgaagtt catgccggaa gaacgtacgc
     6121 gggttttcca ctcgccgtag tcatcggcaa aggccatcaa tgagaagtgc aatggcaatg
     6181 tgaagccaag aatcacgcca ttaccgagga tcaccgccag ccacagcgtt tgataagccg
     6241 ggccggaggg caggaaccac atcaacaccg ccagcgcagc gaggagcagg ttggtgtagt
     6301 aataaatttt gacggtatcg aaacgccgcg ttaacgggct gacaatcacc gagccgataa
     6361 tggaggcgaa ggtgaccatg gtgaagaaca gcgatgtgta gcccgtactg ccttgtaaga
     6421 cgtaggtaat gaagtacata tacccaccgc cgcgaatatt aaagacgtta atcagcagga
     6481 aagacatgac cagcatcagc agcagttggt cgttgttccg aagcccggca agatgttcgc
     6541 gcagggtaaa tttccccatt gtggagagcg gcacccgttc acgaacccag aaaaaacagc
     6601 acaggaacat caccacggca atggcgcaca atacgccgac gcccagttga tagccgcgtg
     6661 cagcgttgcc ctgaccgaag agatctacca tccacggtaa gcctaccgaa accagaaatc
     6721 ccgctacgcc gcagagaacg aatcgccagg actggcagga gatcacttca ttgtggcggg
     6781 tggtcatggt gttgatcagc gcgcagtacg gtacgttgat ggcggtataa ccgactgaaa
     6841 ggagcaggta agtaccgaac gcccaggcga tttttactcc catgctggca tctggcacgg
     6901 tgaacgtcag gatgccgata atgccgatgg gaatggcaat ccatagctgc cacggacgaa
     6961 agcgccccca tcggctttgc gtccggtcgg caatgacgcc catcaccggg tcggagatgg
     7021 catcaaacac gcgcagtgca ataaacagcg tgccaaccag cgccggagtt aatccaaaaa
     7081 cgtcggtgta gaaaaaggtg agaaaattca tgatcaggca agttattacg gtgccgccag
     7141 cgtcacccag gccgtagcct attttttccc gtacagataa cgcatcgtca attttacctt
     7201 tagcgatatc agcggtagta atcggtgtag aggtcataga tattcctcgt gaataggaag
     7261 caatttttag ttttattgaa atgacctgat taattctgcc gtgaacggga atatcaacaa
     7321 ggcgagaaaa gagtaaaaaa aggtgacgat tccgacctga atttaaaagt gtgaaatgga
     7381 tcaggtgaaa ttaatgtttt tgattaataa tcaaaaggta atgtgtttaa tcattaatga
     7441 ataagcggga aattacatat caatgctcga attatccatg acgttaccga ttaaagtgca
     7501 aaatggtgga ttatttattt cccgcggcgt agggaggcat cctgcaaggc ggctggagtc
     7561 atgggaaatt atctttgtcg aaaaaggtcg attaacgatt caggaagaag aacggatatt
     7621 tctggtcgat gcgggagaga gtttattact ctggccgaat cgacaacatg ttggggtgga
     7681 ggattttccc gccgatctca aattttactg gctgcatttt gaggttaaag cgccggatag
     7741 cgacccgcgc tggctcactc atcttagcgt gccgcaacaa acgaaggttg ccgatccgca
     7801 ggcgctggtg gccctgtttc gccagttctt aagcgaacag gaaaagcatc aacgcactcc
     7861 ggcgctggag tttattgtgc tgctgatttt gcagcagctc acccgtgacg cgcgtcttga
     7921 tgaacaggcc gatgccgcag gtaccacgct tgcctggaaa gcgcagaaac tgattcgcac
     7981 gcaatatcac cgcccgcttt ccacggcact tttagcccgt gagctgtact gcaatgccga
     8041 ttatcttggg cgcgtctacc ggcgggtgtt tcatctgacg ttaacagaag caatccaccg
     8101 ccagcgggtg cgcgaagccg aaaagctgtt aattagcgaa gcgcgatcgc tcacggaagt
     8161 ggcggcgttg tgcgggttta atgacgtcgg ctatttccgg cagatttttc gcaagcacac
     8221 cggtttaacg cctgccgcct ggaaacggcg ttacagcaag gaacatatca attcgtagtg
     8281 ccggggcgat gaagccccgg tgtgagggat tactgcccgt aataggcatt gtctccatgc
     8341 ttacgcaggt agtgcttatc cagcagttcg ttttgcatgg cagggagttg cggcgcaagc
     8401 tggcgcgaga acaggcccat ataggcgcac tcttccagca ccacggcgtt atgcaccgca
     8461 tcggcggcat tttttcccca ggcgaacggg ccgtgagaat gcaccagcac cgcaggaatt
     8521 tgtgccgggt tcaacccacg ttcttcgaag gttttaatga tcacttcgcc ggtctggtat
     8581 tcatactcgc cgttgatctc ctctgcggtc atcaggcgcg tgcaggggat ggcaccatag
     8641 aaataatcgg cgtgcgtggt gccccaggcg gggagatcca gcccggcctg tgaccagatg
     8701 gtcgcgtggc gcgagtgggt atgcacaata ccaccgattt cggcatagcg acggtagagc
     8761 gccagatgcg ttggcgtgtc ggacgagggc tttttgctgc cttccaccac tttaccgctg
     8821 gctatctcaa ccaccaccat atcgtcggcg gtcatcacgt cgtactcgac gccggaaggt
     8881 ttgattacca tccattgccg cgtttcgtct accgcgctga cattgcccca ggtgaacgtc
     8941 accagatggt gagcgggaag cgccagattc gccgccagca cgtcggcttt cagttgctct
     9001 aacatataaa tccggcctcc tgcatacgcg cttcaatcca gcgccgcgcc tgaataatct
     9061 ccagcaccgg ctctttggct ttttcggtcc acatctcaat caggaaagaa ccgcgatagt
     9121 tcagttcatg cagcgttttg aagatgccaa cgaaatcgac gcagccttcg ccaaacggca
     9181 cgtcgcggaa ctgtccggga ctttgctcgg taactggctg ggtgtctttc aggtggatcg
     9241 cggcgatacg gtcaatgccc agtttcagtt cggcgggaac atcattgccc caggcgctga
     9301 ggttgccgac gtccgggtag acggtgaacc acggcgaggc gagcatctcg tcccactttt
     9361 tccatttact gatagagttc ataaacgcgg tgtccataat ctccaccgcc agcatgactt
     9421 gtgacgccgc cgcctgttcg accgcccacg ccagcccttc agcaaaacgt tgctgggtgc
     9481 cttcgtcgtg gtcttcgtaa tagacgtcat aacctgccag ctggatggtg cgaatgccga
     9541 gatcgcgcgc caggcgaatc gctttactca tgatttcccg cgcccgttcg cgcactgcct
     9601 cgtcccggct gccaaaggga aaacgacgat gggcagacag gcacattgac ggaatggcaa
     9661 cacccgtttc gatcatcgca gtgaccagcg aagcacgttg tgcggcgctc cagtcaagac
     9721 gtgagagccg ttcgtcggtt tcatccaccg acatttcgac aaaatcaaag ccacagcttt
     9781 ttgccagcac cagccgctcc ggccaggaga gatctttcgc cagcgctttt tcataaattc
     9841 ctaacggatg attacgcacg cgcgcctccc cagatagcat caatttgcgc atggaaatcg
     9901 ccagccacct gcgccggatt tgctgcgcct gccagcgccc gcccggcaat aaacgctttc
     9961 acgcggatat ctttaaacag cggaaggtca gcaggagtaa tcccgccggt aatggaaagc
    10021 tcaaggccga tatcggaaag cgccttcatg agtgccagat cggcttcgcc ccactgttgc
    10081 cctctggcct gtgcatcacg accgcgatga taaatcgcct gccgcacgcc aatacgatgc
    10141 cagtcgcggg cgtcgtccag cgtccagtta ccgaacaact cgatctgaat ttcaccaccg
    10201 cagcgttgcg ccatcgtgtg gcctttttcc accgtcgcga gcggcgcggc gcagatgatg
    10261 gtcatccagt tagcgcctgc gccaaacgcc tgttgcgcga gcgtttcacc agcgtcggcg
    10321 accttccagt cggcaacgat gattttgtcc gggcattgtt cgcgcaaggc tttcaccgcg
    10381 ctaagccctt cgtttaaaca gagaatggtg cccgcttcga cgatatcgac gctgtctttt
    10441 aacagcgtca cgtcgcgctg cgcggcttca agtgatgagt ggtcgagtgc cagttgcaga
    10501 agtggtcggc tcataatgtg tgctccttga tgcgggcgtg aaagccctga agtgcggcaa
    10561 tgagatgctg atagcgttgg tatttttgtt ggtaaagctg atgcgcggtc atatccggca
    10621 gcagggtgcg taccgggtgc tgtaagtcac gttgagcttc gctgaaatcg cgataaactc
    10681 cggtgccgac gcgggcggca agtgccgcgc caaaacagcc ggtttcttcc acctgcggca
    10741 gctcgatacg cagaccgctg acgtccgcca gcatttgcat ccagacatcg gagtgcgccg
    10801 ggccgccagt gacgcgcagg gtgtgcacat cagtaaaacg ttcgcgcatc cggttgaggt
    10861 gggtcatatg gctaaacacc accccttcat agatggcctg caacaggtgc gcgcgggtgt
    10921 gaatggcctg catcccgtag aaaccgctgg tcatctcgag tccggcgttg ctgccgtaca
    10981 ggaacggcag gaaaaagaga tcgccaccga ccttcggcaa gctggcaacg gcctgattga
    11041 tctcatcaaa cgagatttct ccccactgcg cggtaaacca ttcgaggttg ccggaagagg
    11101 tagggctggc ttcgtgaacg ataaattgac catcgttaac gtagcgacca tagacatacg
    11161 gatgcgcttc accgtcacgt aaaccgcggg ttatgccgct ggtcaccgcc caggtgccca
    11221 tcaccgcatt aagggtaaat tcgtcttcga tcccggcaca gagtgcggtg gaaaccacat
    11281 caaacaggcc gccgacaacg gacgtacccg ctttcagacc ggtcagtgcg gctgtctgag
    11341 cggtgatctc cccgcagatt tcggcagaac cgacaacagg cggcagggcg tgattgattt
    11401 cagcgatccc cagccagtcg gtgaggcacg ggtcatattc cccaagactc atgttataga
    11461 ggttggactc ggaaatattg ctctcttcac agcctttgac gccggttaaa caccagcgca
    11521 ggtagtcgtg cgtcatcatc acgcagccaa tttgcgcgta gcgttccggt tcgtgctctt
    11581 tcagccagcg taacagcgac accggatgcc cggtccacaa ggtttgtcgg gtcaacgggt
    11641 agagtttttc cgggatgcca tcttcctgcc agcgacgaac gatttccatc gcccggcgat
    11701 ccgaggacaa aatagcattc ccgagcggct tgtcgttttt atccagcaaa aacaagccct
    11761 ttccctgtgc ggagataccg ataccgacaa tttgttcccc actaacacca gaatgagtaa
    11821 gcagggcgcg aatgacagcc atgcagcatt gccacagttc tgccatatcg cgctctgccc
    11881 aacctggctg cgggcttaat gcgcacagcg gcaggcgctg cacgcctgcc tcccggcctt
    11941 cgcggtcata cagcccggct ttcagccagc taccgccaca atctaacccc agccagtatt
    12001 gcgtcatggt ttattgcacc tcatccacgg ctttcagcag atcagcgcca tgcggcacat
    12061 ctttaacaaa catgtcgcgg acctgagtac ccagcgcgtc gctaaaggct ttgcggtcga
    12121 gatcggtgat gacttccacg cccgcttctt tcatgccgtc gatgattttt tgctgatctt
    12181 cggcaaccag tttgcgctgg tagttacccg cttcctgcgc agatgaaatt agcgcttgct
    12241 ggaactccgg gcttaagcca tcaaacttcg ctttgttgat caccaccaga agcggggaat
    12301 aggcgtggtg cgtcagagaa aggtacttct gcacttcgaa aaattttgct gaccagacga
    12361 cgttgatcgg gtgttcctga gcgtcgatag tgcgggtttc cagcccggta tagacttcgg
    12421 caaacggcat cgggatcggg ttagcgccaa agactttgaa tgcggcgata ttcatcgggc
    12481 tattgttggt gcgaattttc agccctttca ggtcggcggg ggttttaacc ggtgcgcgcg
    12541 agttggtgac atcgcgccag ccgttttccc agtaggccag tacttttagt cctttacctt
    12601 caagtgaggc tttcagatca tcaccgactt tgccgtcgag cgttttatgc gcgtgagcgg
    12661 tatcgcggaa caggaaaggg acatcaagca agttcatcac tggtgataac ccggcaaagt
    12721 tattcgagcc ggacatttcc atatcgatgg tgccgccacg tacgccgctg atcatcgcct
    12781 gcgcgttacc gagggtgctg tccgggaaca attttaattt cagttcgcct ttggtttttt
    12841 cctgtaataa ttcattgaat tttttcgccg caatatgttg cgagtcggtt tgtgatgtct
    12901 cataaccgaa acgtaatgat tgtgccgcca gagaagatgt gctgaatgca gccaggccag
    12961 caatgaataa tgcgtaggtt acagagcgta atttcataat attttccttt taattaatcc
    13021 atttcaaagg gaggatgatg agatcgggaa taaaaacaaa tacgactaat aaggaatata
    13081 aaacgaggac gtaagggaaa acgcctctga ctgcatcatc gaatttgagt tttgccaccc
    13141 ctgaaataac gttaagcaca ttaccgatag gcggtgtaat taagccgatt gagcagttaa
    13201 tgataaacat gacaccgaaa taaatcggat cgattcctgc ctctttaact aaaggcatta
    13261 ataccggggt aagaattaat accgtcggcg ttaaatccat gaccatgccg acaatcagga
    13321 tagccaccat aatgacgata aacagcagac gcggtgaatc gaccaacggt tgcagtaaat
    13381 cagaaaccat catcggcagt tcagcaatgg taatcagcca ggcggaaact tgtgccgagg
    13441 ccaccagaaa cataaccact gaggtggttt tcgccgcgcc aatgagtaca tgccaaagtg
    13501 tggcgaaggt catttcacgg taaataactg tggcgacaaa cagcgcatag aaagcagcaa
    13561 cggccccggc ttcggtaggg gtaaacagac cggagcgaaa cccgccaata atgatgactg
    13621 gaagaaacag cgcccagata ccggagacaa aagagtgcca gatttcctgc atcgttgctt
    13681 tttgctggcg gggtaaatta aggcgactgg cctgccacca ccaggtaagc atcagcgttg
    13741 cgcccatcat catgccaggg gcaatgcccg ccataaacag cttactgatg gataatccgc
    13801 tggaaacgcc gaagataata aacggaatgg aagggggaat aattggcgcg ataatgccgc
    13861 cagaggcaat cagccccgcc gcccggttga ccgggtagtt ggcgctgcgc atcatcggca
    13921 ccagtagagc ggcaacggcg gcggtatccg caacggcaga accagaaagg ctcgccataa
    13981 tcatagccgc cagcacgccg acgtagccca gcccgcctgg tttatgcccc accagtttca
    14041 tcggcaggtc aacaatgcgt tttgacaagc cgcccgcatt catgatttca cccgccagca
    14101 caaagaacgg aatcgccagc agggaaaagc tgtcggctcc gttcaccagc gtttgtgcca
    14161 tgatctggac atcaaacatg tccagccaga acattaacgc cgccccgcac aacaacagtg
    14221 cccaggcaat aggcaaaccg atagcaatac cacccaacag acagcccaga aaaatcagca
    14281 cagccatgat taagctcctt gcggtggcga cgttagagaa ttgctacgcg tgatgagttg
    14341 atataaatga cgcagttcaa agaatgcgat aacgaggctg gtgggcagac aagcggcata
    14401 catcaggccg atgggcaaac cgaggatcgg tgaataatcg ctccagtcct gaattgtttt
    14461 tagcgttgcg ccccaggcca gtgcgccaca aataaataag attaaggaat gggtaaccag
    14521 agcgactcgt cgttgccatg cgggggagag tttctccacc agaaaggtga cctgaacgtg
    14581 ggcgttatcc ataaaagcca caatcgcgcc aataaatgtt aaccagacaa ataaataacg
    14641 tgacaattca tcaacagata aaatgcttgt ctgaaaacca tatct
//
LOCUS       DAZGSP010000123        14466 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZGSP010000123 DAZGSP010000000
VERSION     DAZGSP010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.124"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..172)
                     /locus_tag="ACHK74_004289"
     CDS             complement(<1..172)
                     /locus_tag="ACHK74_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFP0223002.1"
                     /translation="MKKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLPP"
     gene            429..713
                     /locus_tag="ACHK74_004290"
     CDS             429..713
                     /locus_tag="ACHK74_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223003.1"
                     /translation="MNMCDSKYTLISGEGGLRADCADYLFFAVYAIALLMIILWMTAS
                     VIYSIEEYTSQEKYGKSKVYSYFWFFIWFFVFNCAVYSSLKAINIKDIIS"
     gene            complement(706..1593)
                     /locus_tag="ACHK74_004291"
     CDS             complement(706..1593)
                     /locus_tag="ACHK74_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223004.1"
                     /translation="MSTSSFIFAILPGILMICIITSLFWVVIYARGKWTFSSGITVFR
                     YLQQYRWFHFGNISLLSLSVILTTRVYDTEYPLQVYVCIYIPPVMILTSLLTILTIKE
                     ATRTYKTYKSNRLTPVSVIAYAGLGYMFCFTTGIYELSSTRLNPVISDMLMSFRSRLS
                     IMLPVTLAEIAVIYLLLLALHFPDGVSARGFSVTRYLRHNDWFYYINTGNAILALILS
                     TWIYHSSISLPAALFTGALSIFFNTTIPASLSVIEHTPSDTDSKIWDTRKTSPATVLY
                     CSGTGYVIGFIVNPSAAIS"
     gene            complement(1786..2028)
                     /locus_tag="ACHK74_004292"
     CDS             complement(1786..2028)
                     /locus_tag="ACHK74_004292"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFP0223005.1"
                     /translation="MSAEKFRVVIYTNTCEKKVSYIPATEDINIAIMPFQTADNGLII
                     VCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            complement(2109..3803)
                     /locus_tag="ACHK74_004293"
     CDS             complement(2109..3803)
                     /locus_tag="ACHK74_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFP0223006.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTARVHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIDGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTTPDNSEPQQV"
     gene            complement(3830..4450)
                     /locus_tag="ACHK74_004294"
     CDS             complement(3830..4450)
                     /locus_tag="ACHK74_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFP0223007.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            complement(4717..5382)
                     /locus_tag="ACHK74_004295"
     CDS             complement(4717..5382)
                     /locus_tag="ACHK74_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFP0223008.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLES
                     ARQMSAFRGMAEEFTTLRKLLATNTDIPVANTLKSLLESGNNAALYDLYRFRSALAAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            complement(5523..6029)
                     /locus_tag="ACHK74_004296"
     CDS             complement(5523..6029)
                     /locus_tag="ACHK74_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005082529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFP0223009.1"
                     /translation="MNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILK
                     KVRRTDKVCGYRRHIHAVFPGYFFLKADPERHSFTHLRRHSAFLDFVKMAGEIKTVRE
                     DIVQSLMKVYPDPALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHL
                     VSDDGALT"
     gene            6864..7736
                     /gene="repA"
                     /locus_tag="ACHK74_004297"
     CDS             6864..7736
                     /gene="repA"
                     /locus_tag="ACHK74_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFP0223010.1"
                     /translation="MAALNQTSFTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALF
                     DSLDISEEAVASARRSRVEWENKLRKKQGLDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASP"
     gene            8431..8621
                     /locus_tag="ACHK74_004298"
                     /pseudo
     CDS             8431..8621
                     /locus_tag="ACHK74_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8694..8945
                     /locus_tag="ACHK74_004299"
     CDS             8694..8945
                     /locus_tag="ACHK74_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP0223011.1"
                     /translation="MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSNQPLHDVDLDSYL"
     gene            8942..9229
                     /locus_tag="ACHK74_004300"
     CDS             8942..9229
                     /locus_tag="ACHK74_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0223012.1"
                     /translation="MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKREHSNVYNLASERMR"
     gene            9333..9923
                     /locus_tag="ACHK74_004301"
     CDS             9333..9923
                     /locus_tag="ACHK74_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFP0223013.1"
                     /translation="MMKNPLAYFHIATFLLSGKSRNLSEYWKAEVIMRDKFVLHRLIR
                     NGLQRQRGFLMWWRLANEMFISGNKKQRKCAIKIKNALMERYGCDIGLGARIGKGLML
                     PHHAGIVIHGNVDIGENVIIRQNTTIGEKVSDSRENYIVIGDNVDIGAHTCIIGLNVK
                     IGSNVKIGAMSFIMEDVPDNCTYVTRKESRVIIREQ"
     gene            10026..10664
                     /locus_tag="ACHK74_004302"
     CDS             10026..10664
                     /locus_tag="ACHK74_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFP0223014.1"
                     /translation="MGSVTKKPSPVVIIKKKRRILSHQDDAVQAVPDDIKPAPVMEFK
                     TDSLPPEIPATVAGSPEQQKKTGRKKKKKKKNNRWNHEFIRHSLDCVKKTFPQLRAEE
                     GGYHAFKIGISKDVMAFISASPECGLTVKEWNCAVRMITGGYKYLQRISVSGTPRYGL
                     DGKVHGTVTEEEALYAQAKLEKHTQRYNARMKETEDARCNQPSTNCQHRTAI"
     gene            10751..11065
                     /locus_tag="ACHK74_004303"
     CDS             10751..11065
                     /locus_tag="ACHK74_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223015.1"
                     /translation="MITVIISEVDGWREWKHRARTKDAQTAIIRAMNKHFPRSYNFIP
                     DDIDNAPVLFAAVTRTPNVKITGHIWKPMWNRGICWNVKGPPVIITLIQGAAWNSENK
                     PR"
     gene            complement(11312..11452)
                     /locus_tag="ACHK74_004304"
     CDS             complement(11312..11452)
                     /locus_tag="ACHK74_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFP0223016.1"
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGL
                     GG"
     gene            complement(11550..11879)
                     /locus_tag="ACHK74_004305"
     CDS             complement(11550..11879)
                     /locus_tag="ACHK74_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HFP0223017.1"
                     /translation="MTNKLFEHTVLYDSGDAFFELKGNASMKLSPKAAIEVCNEAAKK
                     GLWILGIDGGHWLNPGFRIDSSASWTYDMPEEYKSKIPENNRLAIENIKDDIENGYTA
                     FIITLKM"
     gene            complement(11869..12129)
                     /locus_tag="ACHK74_004306"
     CDS             complement(11869..12129)
                     /locus_tag="ACHK74_004306"
                     /inference="COORDINATES: protein motif:HMM:NF023528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E5-related ribonuclease"
                     /protein_id="HFP0223018.1"
                     /translation="MPPGNFGVSGVQPLSNGATGTSFDKRSPKKTPPDYLGRNDPATV
                     YGSPGKYVVVNDRTGEVTQISDKTDPGWVDDSRIQWGNKNDQ"
     gene            complement(12506..13372)
                     /locus_tag="ACHK74_004307"
     CDS             complement(12506..13372)
                     /locus_tag="ACHK74_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP0223019.1"
                     /translation="MIKGVLFAFLAAFSWGAAIVMSKKGLENMDAGGLFFWQVGSAAM
                     LSWFVLAISRKKLPVTKKSILAYSTGIFEPFLAYTFTLYGLKFISAGITAVIFSLESV
                     FILILSVILFFVKINSPLHFILLLTGAMAGSIMVALPDINDRIDNITGYILIVAGVLS
                     ASFYVVISSRLIVNFEPVTLLTGQLTFSFILACFFIVLTGTSLQLPVESVFLVFLSGI
                     LQYFLAFCFYLYSLRWIPVHIAGAMLYFIPLVALLLSWFFLQEDISFIQGLGVFITIL
                     CVYILNKKYAIE"
     gene            complement(13365..14432)
                     /locus_tag="ACHK74_004308"
     CDS             complement(13365..14432)
                     /locus_tag="ACHK74_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223020.1"
                     /translation="MNKPLHQHINNLPQLPSDFSFGKDIDSIHKFIFKETDDNIIEKE
                     LRKWASVNQPCVFGKLACKKIKGLDFHLSIIKDPSLYTNDSKLFEFLRRERIIFKERA
                     LKGEVSAHLIYFIHQKIAFAKPSKELVDIQKHICSLHMPEYYPVREDIIYTESIPLQE
                     NENVMVYKAGVNIFYSSAHRTRNHDRRIPGGMLISVNAPGHFMRLAIAKGFYKDQEQA
                     LSDIRNMTIQSIGKGGYSHPEKISTTWHSDNKMNRFGCPIHSTNSDYYSGFYHTDVLI
                     PGELTQDSRIIHNVDSNDPMIFNWNVLFYVSLEKFPVDNPYYGEFLGIPVDEEAKFFN
                     PFPPRAFENKPLYNEEITKYD"
BASE COUNT         4081 a         3157 c         3389 g         3839 t
ORIGIN      
        1 gtggcggaag cagacgtttt tgcgtatggg cgataatggc agcgctttcg cgaatttgtc
       61 cgtcgacgaa caccagagcc ggaggcgtgg tgtcaggaac aggcgtggca ttaaatgccg
      121 tacagccgct gagtaatgac ggcagaagga gtgtaagtga tttctttttc atagaataaa
      181 tccggtatgt atcgggttac ggtataagac aggtggctgg taacaggtct tcagataaat
      241 ataatttcat caggatattg atgatgaaca ttcatgttcg gggtgattat gaaaactaaa
      301 acattccggt tccggaaata taaaagaaaa acgtcctgct gcaaatgata gtttccagaa
      361 aacaagtgat attgttacgc aatattatga cggactcttt attattttgc tcggtaggtt
      421 ttgtgtttat gaatatgtgt gattcaaaat atacgttgat ttctggtgag ggtggtttac
      481 gtgctgattg tgccgattat ttattctttg ccgtatatgc aattgcgttg ctaatgataa
      541 ttttatggat gacagcttca gttatttatt ctattgaaga atatacgagt caggaaaaat
      601 acggtaaaag taaagtctat agctacttct ggttttttat ctggtttttt gtttttaact
      661 gtgctgttta ttcttctctg aaggcaataa atataaaaga tataatcagc taatggcagc
      721 agagggattc acgataaaac caatgacgta acctgttccc gagcaataaa ggactgttgc
      781 cggggatgtt tttcgtgtat cccatatctt tgaatctgta tcagatggcg tgtgttcaat
      841 gaccgataaa ctggccggaa tggtcgtgtt aaaaaaaata cttaacgcgc ctgtaaataa
      901 agcagcggga agagaaatgc tggaatgata tatccatgtg gagagtatca gtgccagaat
      961 agcatttcct gtatttatat aataaaacca gtcgttatgg cgcagatacc gggtaacgga
     1021 gaaccccctg gcggacacgc catccgggaa atgcagcgcc agaagcagga gataaataac
     1081 tgctatttca gccagtgtaa caggcagcat aatggaaaga cgtgaccgga atgacatcag
     1141 catgtcactt atgacaggat taagtcgtgt tgaagataat tcgtaaatgc cggttgtaaa
     1201 acagaacata taacccagtc cggcataagc aataacggaa accggcgtca gtctgtttga
     1261 tttgtatgtt ttatacgttc tcgttgcttc tttaatggtt aaaattgtca gcagggatgt
     1321 gagaatcata acgggtggta tatatatgca gacataaacc tgcaaaggat actcagtatc
     1381 gtatacccgg gttgttaata taacggacag actcagtaag gatatatttc cgaaatgaaa
     1441 ccagcgatac tgttgaagat accggaaaac agtgatgcct gaagaaaatg tccatttgcc
     1501 gcgagcataa ataacaaccc agaataaaga agtaatgata catatcatca gtatgccggg
     1561 taatattgca aaaataaatg atgatgtgct cattattaaa tctccatcag catggtgatg
     1621 cttaaatttt tacggttgtg aggtgcgtaa tcaatatcat gtgtagttgt gaataatagc
     1681 gtcagcaaca tatataaaaa taatctgcat tcctgaaaaa tatcgcggtt gtgaaatatt
     1741 taatttttac ccccttttat gggggatgaa aagcaggctg tcaggctata actgaacgcg
     1801 gtttctggat tttggtgcta atgtcacatc tttccattca cagctgcttc cgggtatacg
     1861 ctctttgcgt cgtattatac gggagttctg acacacaata attaagccat tgtctgccgt
     1921 ctgaaatggc attatagcga tgtttatatc ttctgttgca ggaatataac tgactttctt
     1981 ttcacatgta ttggtgtata tgacaactct gaatttttct gcggacattt tcaatcctct
     2041 tttaaaccag aatatcccgt gagggcattc tttgtgaaaa agatgccgga aaaattccgg
     2101 cacggggatt aaacctgttg tggttctgag ttatccggag tggttaccgg ttcgctgaca
     2161 tctggaacct tttccggggc aggcatgacc agtggacgtg tcagggctgt aagtccttca
     2221 ccggataaac ccacctcttt cagcagggag tcgatgagcg gagcctgtgt acggtaagcc
     2281 agagcggcgg ttactgcctg ttcagcgaga tttccgccac ttatgtcggt tgttgctgtt
     2341 ttaccgcggg taacaccgcc attcagacca tccacctgaa tgattttaat accgtcaatg
     2401 gatttcattg gttctgtgga cttctctatg acggcaggta atgcatccag cagggcaaga
     2461 cggaagcgga ggctggtctg ctcatccgaa agcacgttaa tggcatcgtt cagtgcgcgc
     2521 tgtgcttctg cttctgccag acctttctga cgggtggcct ccgccagttc aataattgct
     2581 gctgcctgga gttcggctgc ttctttttca gcctgtgcgc gaaccgtcag ttcaaccgct
     2641 ttcgtttcag cttcctgagc agcagcaatc agggcaacct gtttggtacg gtcggcctcc
     2701 gcagtctggc gggtggtttc cacatcctgc tgggcctgta cggcctcagc cagtgcttta
     2761 cttgcccggg cttctgcctg tgactgctct tccgattttg ccgccacggc aatggctctg
     2821 gcctggctgg cgatttcggt tgcctgctgt tgttcaattt cccgtatggc cacttcccgt
     2881 tcggcttcga cctggcgggt acgcactatc tgctgtcgtt cgatttcggc ttcctctatc
     2941 ttacgttcag ccagaattcg cgcggattct gcttcacgac gtcgttcagc ttcaatagca
     3001 gcaatactgg cactctgctc tgccgtacgg gtttttaccc gttgttgttg ctccagagtc
     3061 atgaaggctt cctgctgttc aatctccaga cgtcgggaga gtgcatcccg gtttttttcc
     3121 ctgatggcca cttcaacatc ctgctcaact tcgttgcgtt cgcggcgacg acgttctgtt
     3181 tcctgggtca gaagggtcag gccttcagca tcaaaggcat tgtcaggatt aaaatgtacc
     3241 cgtgcagtct ggttgaaact tgtcagcgac acactttcaa gctccagacc gtttttggat
     3301 aagtcttccg caacggtgtt ctgcacaccc tgaacaaagt tctcgcgggc atcctgtaaa
     3361 tcctgcatgc tcattctggc cgccgtggcc cgcagggcat caacaaattt atcctcgacc
     3421 agactacgca gatcttcagg tgtgagcgtg cgttgtccca gcgtctgggc tgcagtggat
     3481 attccttcgg ctgatggttt tacccgaaga aaaaaggcaa cggcaacatc aacacgcata
     3541 cggtcgcggg ttatgagact ttcagccgct gcacgactga cttccagctt cagggtgttc
     3601 atgttgacgg gaattgtttc gtgaaaaatg ggcataacaa ttgcaccgcc actcattacc
     3661 actttttggc ctcccagacc tgtgcggaca aaggcctgct ctgccgatgc ccggcggtac
     3721 aggcgggcaa atatcagccc gataacgaac agggtgagga ccagcgctat aacggtaaac
     3781 aaccatcccg gaagattaaa catagtgaga acctctgtat atgtcgggat taaagaacat
     3841 ccggccaggg attcagttcg cccagccagc gggaaccgga aagacggcag acaataatca
     3901 ctttgtctcc tcgtctgaat tctttcccgg cctccggctc cagcaacagg taatgaacct
     3961 gcccgaagcg gtctgtgaag cgaccttcac agggggaacc cgccactgcg tgataaccgg
     4021 tgatggtggc catacagccg atgaatgcct cctcggtgag cgccgttgtt tcatcacggg
     4081 gtaaccaggg ggcaatcagt ctgccggtgc ggtgtacgag aggacatgtc agcagcaggc
     4141 aaagtggtgc aaggagaatg ttcggggcag gagcctgcag cagatgaata ataagaccct
     4201 gcaggagaat gccggatata ccaaaaaaac ctgccagcat acagaggacg atcagcaggg
     4261 ggatccgtcc gatgttcagc cagtccagcg cctctcctgt cagcccctcc ggtaaatcag
     4321 gtgtatccat agctgaagag agggagtgtc cgcagagcag ggcgataagt tcagcgatac
     4381 cagtaagcag aacaaaagtc agggctgcca gatagggtgc gttatacgcg gtaagtaacg
     4441 tcagtgtcat ttttcctcct gtggcaggtc agtcaaacaa cattatggcg tgaagcttcc
     4501 gcttctttaa ttgtcagaag aagcatatcg tcagtcatca gaagacaagg ggatgtctgc
     4561 aggaggggat gataacaaca gaagcctcac gccggaatgc gctttatgac aaccggagag
     4621 gacggataat aaaaaatggg acatccacat cagcaaaccg cgatttgcga aaaaaagtca
     4681 gcggagagtg gctgccgttg tggctgtccc ggtatttcag tgtaacaggt gattgctgtg
     4741 atctatgccc cattcggctt tcggtaaacc tgaatgcccc acatgaagaa tgcgtgacag
     4801 catgtttctg ccgctgccgt tcaggctgca ttcgcgtccg tgcgcgagta taaaaccttc
     4861 atcccggaat atctcttcca cttctgaaga ttcgagggat gccggatgaa ttaatgaaaa
     4921 tgccaccggt ctgagcattc tgcccgtggt ggaaaaggtg tctctggtac tgttcagctg
     4981 caggatatga cgtttacctg tttgcgcacg ggaaagtgtc tcactcagca gctggtcgtt
     5041 ccatgcgcac gtgccaatct gccatcccag acttctgcat gcggcaagag ccgagcggaa
     5101 gcggtacagg tcgtataatg ctgcgttatt gccactttcc agcagtgatt tcagcgtatt
     5161 tgctaccggt atgtccgtat tggtggccag cagttttcgc agagtcgtaa actcttctgc
     5221 cattcctctg aatgcgctca tctgacgcgc agattcaagc cagttcagca ggtaacgccg
     5281 gcgacgccgt tcgttgttta tgttgtccgg ggcggtggag agtgcttcga gcatattctc
     5341 cagtaacaga atgacagggt tttcctggga tagaatggtc ataaaggcgt cctgaaaaaa
     5401 tgcccgacag ccgctctccg tacaccgtaa ggtgtcaccg gtgccgggca ttgtggaaac
     5461 agaagggaaa aaaatcccgg ccataaaggc cgggaaaagt cgtcaaatta agacagcaat
     5521 tttcatgtca acgctccgtc atcagatacc aggtgcagga gcagggcgat cctcgactcc
     5581 ggcagaggct gtgcatcaag ccggaggagg tactggtaac gcgcttttgt cagccagagg
     5641 gtggaggcag catccagctc ctccctggct gccggattga gcgcaggatc cgggtacact
     5701 ttcatcaaag actgaacgat atcctccctg acggttttga tttcaccggc cattttcacg
     5761 aagtccagaa aggcactgtg acggcgaagg tgagtgaagc tgtggcgttc ggggtctgct
     5821 ttcaggaaaa agtaaccggg aaacacggcg tgtatatgtc gacggtaacc acagacttta
     5881 tccgtgcggc ggaccttttt cagtatcagg ggtgtccagg gaaggacgtg ttgttcggaa
     5941 agccaggaaa agagatgttc gcggtttttg ccggcaggga tgtactgtgc cagataccag
     6001 ttgcgttgat gtaattcact gatattcaaa atccggaacg ctccgcgaaa atcgatcccc
     6061 gcaggggggc ttcgatgttg ttgtgaacgg cgaatacata aagcatgtgt cggaaacagc
     6121 ggctgaccct cgttcagccc ggcctgcagt tctgacaggc acatgattta cgcactcttg
     6181 taagccttgt ggacggtttc gaggcgggcg aaaaccgaca actcctgcaa acaggattaa
     6241 tggctgataa atatattaca taaggatgta tctgtggcaa gagcgaagat aagcagttaa
     6301 atagatcgtt atatttgata aaacagcatg ttatgctgat ttgtgtgcgg tgtctggagg
     6361 attagatcgt ttaaaaactt taggcgatct cttctactaa aaatgatgaa aaacacagca
     6421 taaaagatcg tttgtggaat tgtctatttt tttgacatag ttcaaaaaaa cgacgttttt
     6481 tagggggtta atgagaggcc tggtaaatca gagaaatcgt gtcggctttt caaagcggtg
     6541 gaaaaggggt atattgcgga tcgttattca gtggcttttg ggatcctcgc ggtccggaaa
     6601 gccagaaaac ggcagaatgc gccataaggc attcaggatg tatggcagaa acgacggcgg
     6661 ttggccgggg ccggaaggct gaaaaagttt cagaagacca taaaggaaaa cccccactat
     6721 ctttcgtcga actttggcgg gctcaagaaa gatagtgggg gcctcacgaa tacgggataa
     6781 gtatatatga aaccgtgtca gagattcaac cctttgcagt gcattaacgc acggcaatct
     6841 gttcccgcca taagcgacag cttgtggcgg ccctgaacca gacctcattt actgaaagca
     6901 cataccgtca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaagat gccggaacgc
     6961 tgaagttctg cgaaaaactg atggaaaagg cggtgggctt cacctcccgt tttgattttg
     7021 ccgcgcatgt ggcgtatgcc cgttcgcgcg gcctgcgccg gcgtatgccg ccggtgctgc
     7081 gccgccgggc gattgatgcg ctgctgcagg ggctgtgttt ccactatgat ccgctggcca
     7141 accgcgtcca gtgctccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     7201 aggccgggaa actctccatc acccgtgcca cccgtgccct gaagttcctg tctgagctgg
     7261 ggctgataac ctaccagacg gaatacgacc cgacaattgg ctgtaacatc ccgaccgaca
     7321 tcacgttcac gccggcactg tttgactcac ttgatatctc agaagaggca gtggcttccg
     7381 cacgccgcag ccgcgtggaa tgggaaaaca agctgcgcaa aaagcagggg ctggatgcgc
     7441 tgggtatgga tgaactgata gcgagagcct ggcgttttgt gcgcgagcgt ttccgtagtt
     7501 atcaggcaga gcttaagtcc cacggcatga aacgtgcccg tgcgcgccgc gatgcaggca
     7561 ggacccgtca ggacattgtc accctggtga aacggcagct gacacgtgaa atcgcggaag
     7621 ggcgtttccg gggaagcctg gaggccgtga agcgcgaaat cgaccgtcgc gtgaaggaac
     7681 gcatgataat gtcgcggaac aacaactaca cacggctggc cacagcctca ccctgagttt
     7741 gacctcatct gaataatccg gcctgcgccg gaggcttccg cacgtctgaa ggccggcgac
     7801 gcacaaaaaa gcagcgcagc acacaaaaaa caactttacc atccaccttc aggtgcatcc
     7861 ggttccccct atttttgatg caaaacacgt ctcactgaag gggaattccg tttatccaca
     7921 tttaactgca agggacttct ccataaggtt acaaccgttc atgctataaa gcgccagccg
     7981 ctggcgttac agggtgcaat gtttctttta aaccccagta ctgtatttcc tttaaactac
     8041 ttaattactt ccatttaaaa agaaaaccta ttcaatgcct gtcctgtgga cagacagata
     8101 tgcacctccc acgctaacgc gcgggccccg aacctcaccg ttctgaaacc acagcaaaaa
     8161 gacatcagga ataaaaacac cacacaaacg cagcaccgtg cccatccctc ataactgaaa
     8221 agcgccgccg cccccgcccg aagggcggga acaacatcgc ttttaattat gaatgttgta
     8281 actaaacatc ttcatcgctg tcagtcttct ggctggaagt attgcgtaca cgctcgtaag
     8341 cggccctgac ggcccgctaa cgcggagata cgccccgact tcgggtaaac cctcgtcggg
     8401 accactccga ccgcgcacag aagctctgtc atggctgaaa gcgggtatgg cttggcaggg
     8461 ctggggatgg gtaaggtgaa atctatcaat cagtaccggc tgacgccggg cttcggcggt
     8521 ttactcctgt gccatatgct acaacggagt gccgccttcc atgccgctca cgcggcatca
     8581 gaacaataaa accagcatta aatgtatttg caaaagtatg agtaatacag acttgttaac
     8641 cggtcctttc agatgtatct ttaagacttg ttaaacagtc tcaaaggtaa gttatgccta
     8701 acatcattct gtctgacacc agtgcaagtg tcagtgaact gaaaaaaaat cctatggcta
     8761 cagtcagtgc cggagatggt tatcctgtcg ccattcttaa ccggaatcag ccggcattct
     8821 actgtgtgcc tgcggaactc tatgaaagga tgcttgatgc gctggatgac caggaactgg
     8881 tgaaactggt tactgaacgc agcaatcagc cactgcatga tgtggacctg gacagttatt
     8941 tatgacttat acagtaaaat tcagggatga tgcgctcaaa gaatggctga aactggataa
     9001 atccattcaa cagcagtttg cgaaaaaact aaagaaatgc agcgaaaatc ctcatattcc
     9061 ttccgcaaaa ctgcgtggat taaaagactg ctacaaaatc aaacttcgcg catcggggtt
     9121 tcgtctggtc tatcaagtta ttgatgacat gctaattatt gcggttgtcg cggtaggtaa
     9181 acgtgaacat agtaacgttt ataatctcgc tagtgagaga atgagatagt tatctttaaa
     9241 gctgactgat attactttaa aagtatttct gatagttatg ttattttatt ccacaataca
     9301 gaatattttc tttattcttt aaggtcgttt ttatgatgaa aaatccatta gcttattttc
     9361 atattgccac ctttttattg tcaggtaaaa gtcgtaatct gtcagaatac tggaaagccg
     9421 aagtcatcat gcgggataag tttgtcttac accgcctgat aagaaacggg ttgcagagac
     9481 aacgaggctt tctgatgtgg tggcgcctgg ccaatgagat gtttatctct ggaaacaaaa
     9541 aacaaaggaa atgtgcgata aagataaaaa atgcacttat ggaacggtat ggatgtgata
     9601 tcggcctggg cgcgcggatt ggtaaaggcc tcatgttacc tcatcatgca ggaatcgtca
     9661 ttcatggtaa tgttgatatc ggtgagaatg tcattatacg gcagaataca accattggtg
     9721 aaaaggtttc tgacagtagg gaaaactata ttgttattgg cgataatgtc gatattggtg
     9781 cccatacatg tattattgga ttaaatgtga aaatcggaag taatgttaaa atcggggcaa
     9841 tgtcttttat tatggaagat gtacctgata actgtactta tgtcaccagg aaggaatcca
     9901 gagtgataat tcgcgaacag taatgaaata catgttatca ttcttctgtt ggtgactttg
     9961 ccgactcaga tatatactat ctgtatgtcg tgcattatat attaacgata cgcagaggtg
    10021 tgtatatggg cagtgttaca aagaaaccgt cacctgttgt tattattaag aaaaaaagac
    10081 gaattctttc tcatcaggat gatgccgtac aggcagttcc tgatgatata aaaccagcgc
    10141 cagtaatgga atttaaaacc gactcattac ctcctgaaat accagccaca gtggccggtt
    10201 ccccggaaca acagaaaaaa acaggacgta agaagaagaa aaaaaagaag aacaatagat
    10261 ggaatcatga gtttatccga cacagtctgg actgtgttaa aaaaacattc cctcagttac
    10321 gggctgaaga ggggggatat catgcattta aaataggcat ctcaaaggat gtcatggctt
    10381 ttatttcggc cagtcctgag tgtggactaa ctgtaaaaga atggaattgt gctgtaagga
    10441 tgattactgg cggttacaaa tatctgcagc gtatatcagt ctcaggtact ccgcgttatg
    10501 gtctggatgg caaagttcat ggaactgtaa cggaggaaga agcgctgtat gcgcaggcaa
    10561 aacttgaaaa acacacgcag cgttataacg ccaggatgaa agagacagaa gatgctcgtt
    10621 gcaaccaacc atcgacaaac tgtcaacacc ggacagcaat ttgaaggacg cctgcattca
    10681 taacggggca taattatgtg aagtgctgtc ccgctatgga atcagaattg agtaacgaca
    10741 cgaggttttg atgattactg ttattatttc tgaggtggat ggctggcgtg aatggaaaca
    10801 ccgcgcccga acaaaggatg cacagacagc catcatccgg gctatgaata aacattttcc
    10861 acgatcttac aattttatcc ctgatgatat tgataatgca cctgtattat ttgcagctgt
    10921 aacacgtaca ccgaacgtga aaataaccgg tcatatctgg aaacctatgt ggaacagggg
    10981 aatttgctgg aacgtcaaag gtccgccagt aatcatcacg ctcatccagg gggccgcttg
    11041 gaattcggaa aataaaccac gttaagacta ttttttagac aaatgacttt caggaatcgc
    11101 taggatggcg ggacaacagc tgttttggtg tccgaaatcg tacggtaaga ggaaaattat
    11161 cgtcttaaaa atcgattagt agacaatgcc gtccgttaag tgccaatttt cgattaaaaa
    11221 ggcaccgttt tgatggcgtt tttctatgtc cattatgttt tgatatatca gacagttact
    11281 tcactaacgt acgttttcgt tccattggac ttcagccccc taatccggtc agttcagctg
    11341 aggatgacgg ggatacagtc ccgccctgaa catcgcggat atagtttgcc tgacatgcag
    11401 caagaataat ggctgcaaga agcaataaaa taatccctgt tatttttttc atactgttcc
    11461 ttgtatatat ggactcaggc attcatccat aaaaacctga aatccaaagg ttgaaatcat
    11521 ccatgaaaca taaaattcta taacactatt tacatcttta acgtgataat gaaagcagtg
    11581 tatccattct caatatcatc tttaatattt tcaatagcca atctattatt ttcagggatt
    11641 tttgatttgt attcctccgg catatcatat gtccatgatg ctgaactatc tatcctgaat
    11701 ccaggattca gccaatgccc accatcaatg cccaaaatcc ataagccttt tttcgctgct
    11761 tcattacaaa cttctattgc agcttttggt gataacttca tagaagcatt tccttttaat
    11821 tcaaaaaagg catcaccact atcatataac accgtatgtt caaataattt attggtcatt
    11881 tttatttccc cattgaattc tcgaatcgtc cacccaaccc ggatctgtct tatcactaat
    11941 ctgagtaacc tcaccagtac gatcattgac aacaacatat ttaccaggag aaccatacac
    12001 tgttgctgga tcatttcgac ctaaatagtc tggaggtgtt ttcttcggac tacgcttatc
    12061 aaaagatgta ccagttgcac cgttagatag gggttgtacg ccggaaacgc cgaaatttcc
    12121 gggaggcaca aattttatgc tcggcaacct gtacgtaaga ccgccgttaa ccggacagca
    12181 aagaaatagc tgtttagagc gaaagaatca ctgcccgtaa cgtatcacat agctggtacg
    12241 gataaattct cacttactgc accataaacg tactcgtcaa caaccgccaa acatgcttca
    12301 aaaatcaaca aattcgctgt atcggaaatt tcggcgtttc cggcgtataa ccccctataa
    12361 atatattgca gcagccaaag tcggataaaa accacattca agaaattacg gtttcatcct
    12421 ttgttcagca gttgaaaccg taagtttctg ttcaaatgac ccgctgatcc atattgtacg
    12481 tttcccaaac gcagccctaa taaagtcatt cgatggcgta tttcttattg agaatatata
    12541 cgcataaaat agttataaaa acgcctagtc cctgaataaa tgatatatct tcctgcagga
    12601 agaaccagga caacagcaat gctaccagag gaataaaata gagcattgct cctgctatgt
    12661 gaacgggaat ccagcgtagg gaataaagat agaaacaaaa agccaaaaaa tattgtaaaa
    12721 ttcctgacaa aaacacaaga aatacagatt ctacaggcag ttgcagggat gtgccagtta
    12781 gaacaataaa aaaacaagca agaataaaag agaaagtcaa ctgcccggtt aacaacgtaa
    12841 caggctcaaa attcacaata agtcgtgatg agataacaac ataaaaggat gctgagagta
    12901 cacctgcaac aataagtata tatcctgtaa tattatcaat gcggtcattg atatcaggta
    12961 gggcaaccat tatactgcct gccattgctc cagtaagcaa taatataaag tgtaatggtg
    13021 agttaatttt aacgaaaaat aatataacag atagtataag aataaatact gactcaagtg
    13081 aaaaaataac ggctgttata ccagcagaaa taaatttcag gccatagagt gtgaatgtat
    13141 acgccagaaa tggctcaaaa attcctgttg aataggcaag gattgatttt tttgttaccg
    13201 ggagcttttt tctgctaatt gcgagaacaa accatgataa cattgcagca ctaccaactt
    13261 gccagaaaaa caatcctccg gcatccatgt tttcgagacc ttttttgctc atcactattg
    13321 ctgctcccca tgaaaaagca gcaagaaatg caaacagaac tcccttaatc atatttagtt
    13381 atctcctcat tataaagagg tttattttca aacgctcttg gtggaaatgg attaaaaaac
    13441 tttgcttctt catctacagg aataccaaga aattctccat aataaggatt atcaacagga
    13501 aacttttcca gtgagacata aaacaataca ttccagttaa aaatcatagg gtcattgcta
    13561 tcaacattat ggattattct ggagtcctga gtcagttctc cgggaattaa tacatctgta
    13621 tgataaaagc ctgaatagta atcactatta gtagaatgta tagggcagcc aaagcgattc
    13681 attttattat ctgagtgcca tgtggttgat attttttctg gatgagaata tcctccttta
    13741 cctatggatt gaatagtcat attacggata tcagataaag cttgctcctg atctttatag
    13801 aatccttttg caattgccag tctcataaaa tgtccaggtg catttacaga tataagcatt
    13861 ccaccgggga ttcgtcggtc atgatttcgg gttctgtgag cagaagagta aaaaatatta
    13921 actccagctt tatataccat cacgttttca ttttcctgca atggtatgct ttctgtgtag
    13981 attatatctt ctcttacagg ataatactca ggcatatgta aggaacatat atgtttctgt
    14041 atatcaacaa gttctttaga gggttttgca aaagcaattt tctgatgtat aaagtagata
    14101 agatgagcac ttacttcccc tttaagagct cgttctttga agataatccg ttctctacgt
    14161 aaaaactcga agagtttact atcattagta tacaatgatg gatcttttat tattgataag
    14221 tgaaaatcaa ggcctttgat tttcttacat gcaagtttac caaaaacaca tggttgattt
    14281 actgatgccc attttcttag ctctttttca attatattat catctgtctc tttgaatata
    14341 aatttatgta tagaatcaat atcctttcca aaggaaaaat cagaaggtaa ttgtggtaag
    14401 ttgttaatat gttgatgaag tggtttattc atattcacta tatatcctca catagtaaaa
    14461 cttaca
//
LOCUS       DAZGSP010000124        14216 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZGSP010000124 DAZGSP010000000
VERSION     DAZGSP010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.125"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..81
                     /locus_tag="ACHK74_004309"
     CDS             <1..81
                     /locus_tag="ACHK74_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="catalyzes the formation of GDP and D-mannose from
                     GDP-D-mannose; also hydrolyzes GDP-glucose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFP0223021.1"
                     /translation="LLASNDVHANSRAYFLAEKRAGVPGL"
     gene            78..1301
                     /gene="wcaI"
                     /locus_tag="ACHK74_004310"
     CDS             78..1301
                     /gene="wcaI"
                     /locus_tag="ACHK74_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFP0223022.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            1304..2740
                     /gene="cpsB"
                     /locus_tag="ACHK74_004311"
     CDS             1304..2740
                     /gene="cpsB"
                     /locus_tag="ACHK74_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFP0223023.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            2933..4303
                     /gene="cpsG"
                     /locus_tag="ACHK74_004312"
                     /gene_synonym="manB"
     CDS             2933..4303
                     /gene="cpsG"
                     /locus_tag="ACHK74_004312"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFP0223024.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLCDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKEKTLGELVRDRMVAFPASGEINSKLAQPAEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            4358..5752
                     /gene="wcaJ"
                     /locus_tag="ACHK74_004313"
     CDS             4358..5752
                     /gene="wcaJ"
                     /locus_tag="ACHK74_004313"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFP0223025.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            5754..7232
                     /gene="wzxC"
                     /locus_tag="ACHK74_004314"
     CDS             5754..7232
                     /gene="wzxC"
                     /locus_tag="ACHK74_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFP0223026.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGASVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRINFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIATGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            7412..8692
                     /gene="wcaK"
                     /locus_tag="ACHK74_004315"
     CDS             7412..8692
                     /gene="wcaK"
                     /locus_tag="ACHK74_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFP0223027.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKHSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            8689..9909
                     /gene="wcaL"
                     /locus_tag="ACHK74_004316"
     CDS             8689..9909
                     /gene="wcaL"
                     /locus_tag="ACHK74_004316"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFP0223028.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKP
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            9920..11314
                     /gene="wcaM"
                     /locus_tag="ACHK74_004317"
     CDS             9920..11314
                     /gene="wcaM"
                     /locus_tag="ACHK74_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFP0223029.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGHTVVVPAGLVCDNINTGIFIPSGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            11472..12467
                     /locus_tag="ACHK74_004318"
     CDS             11472..12467
                     /locus_tag="ACHK74_004318"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFP0223030.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            12710..13603
                     /gene="galF"
                     /locus_tag="ACHK74_004319"
     CDS             12710..13603
                     /gene="galF"
                     /locus_tag="ACHK74_004319"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFP0223031.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            13986..>14216
                     /locus_tag="ACHK74_004320"
     CDS             13986..>14216
                     /locus_tag="ACHK74_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFP0223032.1"
                     /translation="MIKILHIHLSSKISGAQRVSLDEMKTLSNHYSQYMVCSKEGDFT
                     QEADRIGVKTYVIETLVREISPLKDFYSLIKLY"
BASE COUNT         3502 a         3482 c         3881 g         3351 t
ORIGIN      
        1 ctgctcgcca gtaacgatgt tcacgctaac agccgcgcct attttctcgc tgagaagcgt
       61 gccggagtac ccggattatg aaaatactgg tctacggcat taactactcg ccggagttaa
      121 ccggtatcgg caaatacacc ggcgagatgg tggaatggct ggcggcacaa ggtcatgagg
      181 tgcgggttat taccgcaccg ccttactacc cgcagtggca ggtgggtgag aactattccg
      241 cctggcgcta caaacgagaa gagggggccg ccacggtgtg gcgctgcccg ctgtatgtgc
      301 caaaacagcc gagcaccctg aaacgcctgt tgcatctcgg cagttttgcc gtcagcagtt
      361 tctttccact gatggcgcaa cgtcgctgga agccggatcg cattatcggc gtagtgccaa
      421 cgctgttttg cacgccggga atgcgcctgc tggcgaagct ctctggtgcg cgtaccgtgc
      481 tgcatattca ggattacgaa gtggacgcca tgctggggct gggccttgcc ggaaaaggca
      541 aaggcggcaa agtggcacag ctggcaacgg cgttcgaacg tagcggactg cataacgtcg
      601 ataacgtttc cacgatttcg cgttcgatga tgaataaagc catcgaaaaa ggcgtggcgg
      661 cggataacgt catcttcttc cccaactggt cggaaattgc ccgttttcaa catgttgcag
      721 acgccgatgt tgatgccctt cgtaaccagc ttggcctgcc ggataacaaa aaaatcattc
      781 tttactccgg caatattggt gaaaagcagg ggctggaaaa cgttattgaa gctgccgatc
      841 gtctgcgcga tgaaccgctg atttttgcca ttgtcgggca gggcggcggc aaagcgcggc
      901 tggaaaaaat ggcgcagcag cgtggactgc gcaacatgca atttttcccg ctgcaatcgt
      961 atgacgcttt acccgcactg ctgaagatgg gcgattgcca tctggtggtg caaaaacgcg
     1021 gcgcggcaga tgccgtattg ccgtcgaaac tgaccaatat tctggcggta ggcggtaacg
     1081 cggtgattac cgcagaggcc cacacagaac tggggcagct ttgcgaaacc tttccgggca
     1141 ttgcggtttg cgtagaaccg gaatcggtcg aggcgctggt ggcggggatc cgtcaggcgc
     1201 tcctgctgcc caaacacaac acggtggcac gtgaatatgc cgaacgcaca ctcgataaag
     1261 agaacgtgtt acgtcaattt ataaatgata ttcggggata attatggcgc agtcgaaact
     1321 ctatccagtt gtgatggcag gtggctccgg tagccgctta tggccgcttt cccgcgtact
     1381 ttaccccaag cagtttttat gcctgaaagg cgatctcacc atgctgcaaa ccaccatctg
     1441 ccgcctgaac ggcgtggagt gcgaaagccc ggtggtgatt tgcaatgagc agcaccgctt
     1501 tattgtcgcg gaacagctgc gtcaactgaa caaactcacc gagaacatta ttctcgaacc
     1561 ggcagggcgt aacactgcac ctgccattgc actggcggcg ctggcggcaa aacgtcatag
     1621 cccggagagc gacccgttaa tgctggtatt ggcggcggat catgtgattg ccgatgaaga
     1681 cgcgttccgt gccgccgtgc gtaatgccat gccatatgcc gaagcgggca agctggtgac
     1741 cttcggcatt gtgccggatc taccagaaac cggttatggc tacattcgtc gcggtgaagt
     1801 gtctgcgggt gagcaggata cggtggcctt tgaagtggcg cagtttgtcg aaaaaccgaa
     1861 tctggaaacc gcccaggcct atgtggcaag cggcgaatat tactggaaca gcggtatgtt
     1921 cctgttccgc gccggacgct atctcgaaga actgaaaaaa tatcgcccgg atattctcga
     1981 tgcctgtgaa aaagcgatga gcgccgtcga tccggatctt gattttattc gtgtggatga
     2041 agaagcgttt ctcgcctgcc cggaagagtc ggtggattac gcggtcatgg aacgtacggc
     2101 agatgctgtt gtggtgccga tggatgcggg ctggagcgat gttggttcct ggtcttcatt
     2161 atgggagatc agcgcccaca ccgccgaggg caacgtttgc cacggcgatg tgattaatca
     2221 caaaactgaa aacagctatg tgtacgccga atctggcctg gtcaccaccg tcggggtgaa
     2281 agatctggta gtggtgcaga ccaaagatgc ggtgctgatt gccgaccgta acgcggtgca
     2341 ggatgtgaaa aaagtggtcg agcagatcaa agccgatggt cgccatgagc atcgggtgca
     2401 tcgcgaagtg tatcgtccgt ggggcaaata tgactctatc gacgcgggcg accgctacca
     2461 ggtgaaacgc atcaccgtga aaccgggcga gggcttgtcg gtacagatgc accatcaccg
     2521 cgcggaacac tgggtagtgg tcgcgggaac ggcaaaagtc accattgatg gtgatatcaa
     2581 actgcttggt gaaaacgagt ccatttatat tccgctgggg gcgacgcact gcctggaaaa
     2641 tccggggaaa attccgctcg atttaattga agtgcgctcc ggctcttatc tcgaagagga
     2701 tgatgtggtg cgcttcgcgg atcgctacgg acgggtgtaa acgtcgcatc aggcgctgtt
     2761 tgtcggatgc ggcgtgaacg ccttatccga cctacggttc gggtttgtag tcctgataag
     2821 acgcggcagc gtcgcatcag gcaatgaatg cgaaaccgag atgtaaataa cgacaaaaac
     2881 aaaattggcc gtttcggtca gggccaacta ttgcctgaaa aagggtaacg acatgaaaaa
     2941 attaacctgc tttaaagcct atgatattcg cgggaaatta ggcgaagaac tgaatgaaga
     3001 tatcgcctgg cgcattggtc gcgcctatgg cgaatttctc aaaccgaaaa ccattgtgtt
     3061 aggcggtgat gtccgtctca ccagcgaaac cttaaaactg gcgctggcaa aaggtttaca
     3121 ggatgcgggc gttgacgtgc tggatatcgg tatgtccggc accgaagaga tctatttcgc
     3181 cacgttccat ctcggcgtgg atggcggcat tgaagtcacc gccagccata atccgatgga
     3241 ttataacggc atgaagctgg tgcgcgaagg ggctcgcccg atcagcggtg ataccggact
     3301 gcgcgacgtc cagcgtctgg ctgaagccaa cgactttcct cccgtcgatg aaaccaaacg
     3361 cggtcgctat cagcaaatca acctgtgtga cgcttacgtt gatcacctgt tcggttatat
     3421 caatgtcaaa aatctcacgc cgctcaagct ggtgatcaac tccgggaatg gcgcagcggg
     3481 tccggtggtg gacgccattg aagcccgctt taaagccctc ggcgcgcccg tggaattaat
     3541 caaagtacac aacacgccgg acggcaattt ccccaacggt attcctaacc cgctgctacc
     3601 ggaatgtcgc gacgacaccc gcaatgcggt catcaaacac ggcgcggata tgggcattgc
     3661 ctttgatggc gattttgacc gctgtttcct gtttgacgaa aaagggcagt ttatcgaggg
     3721 ctattacatt gtcggcctgc tggcagaagc attcctcgaa aaaaatcccg gcgcgaagat
     3781 catccacgat ccacgtctct cctggaacac cgttgatgtg gtgactgccg caggtggcac
     3841 gccggtaatg tcgaaaaccg gacacgcctt tattaaagaa cgtatgcgca aggaggacgc
     3901 catctacggt ggcgaaatga gcgctcacca ttacttccgt gatttcgctt actgcgacag
     3961 cggcatgatc ccgtggctac tggtagccga actggtgtgc ctgaaagaga aaacgctggg
     4021 cgaactggta cgcgaccgga tggtggcgtt tccggcaagc ggtgagatca acagcaaact
     4081 ggcgcaaccc gctgaggcga ttaaccgcgt ggaacagcat tttagccgtg aggcgctggc
     4141 ggtggatcgt accgatggca tcagtatgac ctttgccgac tggcgtttta acctgcgctc
     4201 ctccaacacc gaaccggtgg tgcggttgaa tgtggaatcg cgcggtgatg tgccgctgat
     4261 ggaagcgcga acgcgaactc tgctgacgtt gctgaacgag taatgtcgga tcttccctta
     4321 ccccactgcg ggtaaggggc taataacagg aacaacgatg acaaatctaa aaaagcgcga
     4381 gcgagcgaaa accaatgcat cgttaatctc tatggtgcaa cgcttttcag atatcaccat
     4441 catgtttgcc ggactatggc tggtttgcga agtgagcggc ctgtcattcc tctacatgca
     4501 cctgttggtg gcactgatta cgctggtggt gttccagatg ctgggcggca tcaccgattt
     4561 ttatcgctca tggcgcggtg ttcgggcagc gacagaattt gctctgttgc tacaaaactg
     4621 gacgttaagc gtgattttca gcgccggact ggtggcgttc aacaatgatt tcgacacgca
     4681 actgaaaatc tggctggcgt ggtatgggct gaccagcatc ggactggtgg tttgccgttc
     4741 atgtattcgt attggggcgg gctggctgcg taatcatggc tataacaagc gcatggtcgc
     4801 cgtagcgggg gatttagccg ccggacaaat gctgatggag agcttccgta atcagccgtg
     4861 gttagggttt gaagtggtgg gggtttacca cgacccgaaa ccgggcggcg tttctaacga
     4921 ctgggcgggt aacctgcaac agctggtcga ggatgctaaa gcgggtaaga ttcataacgt
     4981 ctatatcgcg atgcaaatgt gcgacggcgc gcgagtgaaa aaactggtcc atcaactggc
     5041 ggacaccacc tgttcggtgc tgctgatccc cgatgtcttt accttcaaca ttctccattc
     5101 acgtctcgaa gagatgaacg gcgttccggt ggtgccgctg tatgacacgc cgctttccgg
     5161 ggttaaccgc ctgctaaaac gtgcggaaga cattgtgctg gcgacgctga tcttgctgct
     5221 gatctccccg gtgctgtgct gtattgcgct ggcggtgaaa ctcagttcac ctgggccggt
     5281 tattttccgc cagactcgct atggcatgga tggcaagccg atcaaagtgt ggaagttccg
     5341 ttccatgaaa gtgatggaga acgacaaagt ggtgactcag gcgacgcaga acgatccgcg
     5401 cgtcaccaaa gtggggaact ttctgcgccg cacctcgctg gatgaactgc cgcagtttat
     5461 caatgtgctg accgggggga tgtcgattgt cggtccacgt ccgcacgcag tagcgcataa
     5521 cgaacagtat cgccagctca ttgaaggcta catgctgcgc cacaaagtga aaccgggcat
     5581 taccggctgg gcgcagatta atggctggcg cggcgaaacc gacacgctgg agaaaatgga
     5641 aaaacgcgtc gagttcgacc ttgagtacat ccgcgaatgg agcgtctggt tcgatatcaa
     5701 aatcgttttc ctgacggtat tcaaaggctt cgttaacaaa gcggcatatt gatatgagct
     5761 tacgtgaaaa aaccatcagc ggcgcgaagt ggtcggcgat tgctacggtg atcatcatcg
     5821 gcctcgggct ggtacagatg accgtgctgg cgcggattat cgacaaccac cagttcggtt
     5881 tgcttaccgt gtcgctggtg atcatcgcgc tggcagatac gctttctgac ttcggtatcg
     5941 ctaactcgat tattcagcga aaagaaatca gtcaccttga actcaccacg ttgtactggc
     6001 tgaacgtcgg gctggggatc gtggtgtgcg tggcggtgtt tttgttgagt gatctcatcg
     6061 gcgacgtgct gaataacccg gacctggcac cgttgattaa aacattgtcg ctggcgtttg
     6121 tggtgatccc ccacgggcaa cagttccgcg cgttgatgca gaaggagctg gagttcaaca
     6181 aaatcggcat gatcgaaacc agcgcggtgc tggcgggctt cacttttacg gtggttagcg
     6241 cccatttctg gccgctggcg atgaccgcga tcctcggtta tctggtcaat agtgcggtga
     6301 gaacgctgct gtttggctac tttggtcgaa aaatttatcg ccccggtctg catttctcgc
     6361 tggcgtcagt tgcgccgaac ttacgctttg gtgcctggct gacggcggac agcatcatca
     6421 actatctcaa caccaacctt tcaacgctgg tgctggcgcg tattctcggc gcgagcgtgg
     6481 caggggggta caacctggcg tacaacgtgg ccgttgtgcc accgatgaag ctgaacccaa
     6541 tcatcacccg tgtgttgttt ccggcgtttg ccaaaattca ggacgacacc gaaaagctgc
     6601 gtattaactt ctacaaactg ctgtcggtgg tggggattat caactttccg gcgctgctgg
     6661 ggctgatggt ggtgtcgaat aacttcgtac cgctggtatt cggcgaaaag tggaacagca
     6721 ttattccggt gctgcaattg ctgtgtattg tgggtctgct gcgctccgtg ggtaacccga
     6781 ttggttcgct gctgatggcg aaagcgcggg tcgacatcag ctttaaattc aacgtattca
     6841 aaacctttct gtttattccg gcgattgtta ttggtggtca gatggcgggc gcaatcggcg
     6901 tcacgctcgg tttcctgctg gtgcagatta tcaacaccat tctgagttac ttcgtgatga
     6961 ttaaaccggt tcttggttcc agttatcgcc agtacatcct gagtttgtgg ctgccgtttt
     7021 acctctcgct gccgacgctg gtggttagtt atgcgctggg cattgtgctg aaagggcaac
     7081 tggcgctggg gatgctgctg gcggtgcaaa tagccacggg tgtgctggcg tttgtggtga
     7141 tgattgtgct gtcgcgccat ccgctggtgg tggaagtaaa gcgtcagttt tgtcgcagcg
     7201 aaaaaatgaa aatgctttta cgggcggggt gagttgttgt tttcggttcc gacaacggtt
     7261 gccgggtgca gcgtaaaacg ccttatccat cctacggttc tgtgctcggg acctgtaggc
     7321 ctgataagac gcgtcagcgt cgcatcaggc aaaaagcggc aacgaacaaa acaaacgcag
     7381 cctcacctgg catcaaattt atgaggaaaa aatgaaatta cttattctgg gcaaccacac
     7441 ttgcggcaac cgcggcgaca gcgccatcct gcgcggctta cttgatgcca tcaacatcct
     7501 caatccacac gccgaagtgg acgtgatgag ccgctatccg gtcagttctt cctggctgct
     7561 caaccgcccg gtaatgggtg atccgctgtt ccttcaaatg aaacaacaca acagcgcggc
     7621 gggcgttgtc gggcgcgtta aaaaagtcct ccgtcgccgc tatcagcacc aggtattgct
     7681 ctcacgcgtc accgacactg gcaagctgcg caatatcgcc atcgcccagg gattcaccga
     7741 cttcgtgcgc ctgctgtcag gttacgacgc cattatccag gtcggcggat cgttttttgt
     7801 cgatctctac ggcgtgccgc agtttgaaca cgcgctttgc acatttatgg cgaaaaagcc
     7861 gctgtttatg attggtcaca gcgtcggccc cttccaggat gagcaattta accaactggc
     7921 gaactacgtt tttggtcact gcgacgcgct gatcctgcgc gaatcggtca gccttgatct
     7981 gatgaaacac agcaatatca ccaccgcaaa agtggaacat ggcgtcgata ccgcgtggct
     8041 ggtcgatcac cacaccgaag acttcaccgc cagctatgcc gtccaacact ggctggacgt
     8101 tgccgcacaa cagaaaacgg tggcaattac cctgcgcgaa ctggcaccgt tcgacaaacg
     8161 cctcggcacc actcagcaag cgtatgaaaa agcctttgcc ggggtggtca atcgcattct
     8221 cgacgaaggg tatcaggtga ttgcgctctc cacctgtacg ggcatcgaca gctataacaa
     8281 agacgaccgc atggtggcgc tcaacctgcg ccagcacatc agcgatcctg cccgttatca
     8341 cgtagtgatg gatgaactca acgatctgga aatgggcaaa attctcggtg cctgtgaact
     8401 caccgtcggt acgcgcctgc actccgccat tatctcgatg aattttgcta ctccggcaat
     8461 tgccatcaac tacgaacata aatccgccgg gattatgcaa caactggggc tgccagagat
     8521 ggcaattgat attcgtcatt tattagacgg cagcctgcaa gcgatggttg cggatacctt
     8581 aggccagctt ccggcgctga atgcgcgact tagtgaagcc gtcagtcgtg agcgtcagac
     8641 aggaatgcag atggtgcagt ccgtactgga acgcatcggg gaggtgaaat gaaggtcggc
     8701 ttcttcttat tgaaatttcc gctgtcgtcg gaaaccttcg tcctcaacca aatcactgcg
     8761 tttattgata tgggctttga ggtggagatt gtcgcgctgc aaaaaggcga cacacaaaac
     8821 acccacgcgg catggacgaa atacaacctt gccgccagaa cccgctggtt acaggacgaa
     8881 cctacgggca aagtggcgaa actgcgccac cgagccagtc agaccttgcg cggcattcat
     8941 cgtaaaaata cctggcaggc gctcaacctc aaacgctatg gtgccgagtc gcggaacctg
     9001 attttgtctg ccatttgcgg ccaggtcgca acaccatttt atgccgatgt ctttatcgct
     9061 cattttggcc ctgcgggggt aaccgcagca aaactacgcg aactgggtgt cattcgcggc
     9121 aaaattgcca ctatcttcca cggtattgat atctccagtc gggaagtgct caaccactac
     9181 actcccgaat atcaacaact gtttcgccgt ggcgacctga tgttaccgat aagcgatttg
     9241 tgggccggaa ggctgcaaaa aatgggctgc ccgagggaaa aaattgccgt atcgcgtatg
     9301 ggcgtggaca tgacgcgttt tagcccgcgt ccggtgaaac cgcccgcaac gccgctggaa
     9361 atcatttccg tcgcacgctt aaccgagaaa aaaggcctgc atgtggcgat cgaagcctgc
     9421 cgtcagttga aagagcaggg cgtggcattt cgctatcgca tcctcggcat tggcccgtgg
     9481 gaacgacgcc tgcgcacgct catcgaacaa tatcaactgg aagatgtggt ggagatgccg
     9541 ggctttaaac cgagccatga agtgaaagcg atgctcgacg atgcggatgt cttcctcttg
     9601 ccatcggtta caggtgcgga cggcgatatg gaaggcattc cggtggcgct gatggaagcg
     9661 atggcggtcg gcattccggt tgtttctact ctacatagcg gaataccgga actggtggag
     9721 gccgataaat ccggctggct ggtgcctgag aacgatgctc gcgcactggc gcaacgcctg
     9781 gcggcattta gccaactgga caccgacgaa ctggctccgg tcgtcaaacg tgcacgcgaa
     9841 aaagtcgaac acgattttaa ccagcaggtg attaatcgag aactcgccag cttgctgcag
     9901 gctttataga ggagaacgta tgccatttaa aaaactctcc cgacgcacct tcctgacggc
     9961 aagctccgcg cttgccttcc tccatacacc tttcgcccgt gcacttcctg cccggcaaag
    10021 cgttaacatt aacgactaca accctcacga ctggatcgcc tcatttaaac aagccttcag
    10081 cgaagggcat acggtcgtcg tgcctgccgg attggtttgt gacaatatca acaccggcat
    10141 cttcattcct tctggcaaaa cgttacacat ccttggaagc ctgcgcggca acggcagagg
    10201 gcgatttgtc ttacaggatg gcagccaggt gacaggggag gagggcggca gtatgcataa
    10261 catcaccttg gatgtgcgcg gctctgactg caccatcaaa gggctggcga tgagcggctt
    10321 tggcccggta acgcagattt atattggtgg caaaaacaaa cgggtcatgc gcaacctgac
    10381 catcgataac ctcactgtca gccacgctaa ttacgccatc ttacgccagg gatttcataa
    10441 ccagattatc ggtgccaaca tcaccaactg taagttcagt gatttacagg gcgacgccat
    10501 tgaatggaac gtggcaatta acgacagtga tattttgatc tctgaccatg tcatcgagcg
    10561 catcaactgt accaacggca aaatcaactg gggaatcggc ataggccttg cggggagtac
    10621 ttatgataat aactacccgg aagaccaggc agtgaaaaac tttgtcgtgg cgaatatcac
    10681 gggatcggat tgtcggcagt tgatccatgt tgaaaatggt aaacattttg ttattcgtaa
    10741 tatcaaagcc cgcaatatca cgccggattt cagtaagaaa gcaggtattg ataacgccac
    10801 agtcgctatt tacggttgtg acaatttcgt gattgataat attgaaatga ttaatagcgc
    10861 cgggatgtta atcggctatg gggtaattaa aggcaaatat ctctcgatac cacaaaattt
    10921 ccgagtgaat aatattcagc tggataatac ccaccttgct tataaattgc gcggcatcca
    10981 aatctccgcc gggaatgctg tctcctttgt ggcactgact aacattgaga tgaagcgtgc
    11041 gtcgcttgag ctacacaata aaccgcaaca cctttttatg cgaaatatca atgtgatgca
    11101 ggaatcctca gttggaccag cattgagcat gaacttcgac atgcgcaaag acgttcgcgg
    11161 cgtctttatg gcgaaaaaag aaacactgct gtctcttgca aatgttcatg cggtgaatga
    11221 aagagggcaa agctccgtcg atatcgacag gattaatcac catattgtta atgtggaaaa
    11281 gattaacttt agattgccgg aacggaggga gtagatttgc gaccattcat aaacttactg
    11341 gatataatgt tctaaaagat tataatcctc acactaactg ttaaagctgg ggctgggcag
    11401 cattgactac tcagggaagg tactcatctt tatcttgtgt ttctttcgta acaaccagac
    11461 taaagtgatt tatgaacgat aacgttttgc tcataggagc ttccggattc gtaggaaccc
    11521 gactacttga aacggcaatt gctgacttta atatcaagaa cctggacaaa cagcagagcc
    11581 acttttatcc agaaatcaca cagattggcg atgttcgtga tcaacaggca ctcgaccagg
    11641 cgttagccgg ttttgacact gttgtactac tggcagcgga acaccgcgat gacgtcagcc
    11701 ctacttctct ctattatgat gtcaacgttc agggcacccg caatgtgctg gcggccatgg
    11761 aaaaaaatgg cgttaaaaat atcatcttta ccagttccgt tgctgtttat ggtttgaaca
    11821 aacacaaccc tgacgaaaac catccacacg accctttcaa ccactacggc aaaagtaagt
    11881 ggcaggcaga ggaagtgctg cgtgaatggt ataacaaagc accaacagaa cgttcattaa
    11941 ccatcatccg tcctaccgtt atcttcggtg aacgcaaccg cggtaacgtc tataacttgc
    12001 tgaaacagat cgctggcggc aagtttatga tggtgggcgc agggactaac tataagtcca
    12061 tggcttatgt tggaaacatt gttgagttta tcaagtacaa actgaagaat gttgccgcag
    12121 gttatgaggt ttataattac gttgataagc cagatctgaa catgaaccag ttggttgctg
    12181 aagttgaaca aagtctgaac aaaaagatcc cttctatgca cttgccttac ccactaggaa
    12241 tgttgggtgg atattgcttt gatatcctga gcaaaattac gggcaaaaaa tacgctgtca
    12301 gctctgtgcg cgtgaaaaaa ttctgcgcaa caacacagtt tgacgcaacg aaagtgcatt
    12361 cttcaggttt tgtggcaccg tatacgctgt cgcaaggtct ggatcgaact ctgcagtatg
    12421 aattcgttca tgccaaaaaa gacgacataa cgtttgtttc tgagtaatgc agacgagtca
    12481 gagaaaaaag gaccaggttg gactacgatt attcggaaag aggtactgat taaattatca
    12541 gtatgcggaa ttccgagtaa tccgagtaac ccagatgctg atggtgtgta gtgttatcct
    12601 aaggtttaag caaagttatg cggaactaag ctttggtata tagccataac gtttggctaa
    12661 cctatggcaa taatttacgc actatcagcc acttagaggt aggaaaagaa tgacgaattt
    12721 aaaagcagtt ataccggtag caggtcttgg gatgcatatg ttgcctgcca ctaaggcgat
    12781 tcccaaagag atgctaccga tcgtcgacaa gccaatgatt cagtacattg ttgacgagat
    12841 tgtggctgca gggatcaaag aaatcctcct ggtaactcac gcgtccaaga acgcagtcga
    12901 aaaccacttc gacacctctt atgaattaga atctctcctg gaacagcgcg tgaagcgtca
    12961 actgctggcg gaagtacaat ctatctgtcc gccgggcgtg accattatga acgtgcgtca
    13021 gggtgaacct ttaggtttgg gccactccat tttgtgtgca cgccccgcca ttggcgacaa
    13081 cccatttgtc gtggtgctgc cggacgttgt tatcgatgat gccagcgccg acccgctgcg
    13141 ctacaacctt gctgccatga ttgcgcgctt caatgaaacg ggccgcagcc aggtgctggc
    13201 aaaacgtatg ccgggtgacc tctctgaata ctctgtcatc cagaccaaag agccgctgga
    13261 ccgtgaaggc aaagtcagcc gcattgttga atttatcgaa aaaccggatc agccgcagac
    13321 gctggactca gacatcatgg ccgtaggtcg ttatgtgctt tctgccgata tttggccgga
    13381 actggagcgc actcagcctg gtgcatgggg acgtattcag ctgactgatg ctattgccga
    13441 actggcgaaa aaacagtccg ttgatgcaat gctgatgact ggtgacagct acgactgcgg
    13501 taaaaaaatg ggctatatgc aggcgtttgt gaagtatgga ctgcgcaacc tgaaagaagg
    13561 ggcgaagttc cgtaaaggca ttgagaagct gttaagcgaa taatgaaaat ctgaccggat
    13621 gtaacggttg ataagaaaat tataacggca gtgaggattc gtggcgaaag taatttgttg
    13681 cgaatattcc tgccgttgtt ttatataaat aatcagaata acaacgagtt agcaatagga
    13741 ttttagtcaa agttttccag gattttcctt gtttccagag cggattggta agacaattag
    13801 cgtttgaatt tttcgggttt tgcgcgagtg ggtaacgctc gtcacatcgt aggtatgcat
    13861 gcagtgctct ggtagctgta aagccagggg cggtagcgtg tttatttcat attggtgcag
    13921 ctagtttacc attatataat tgtaagttaa tcacttcgaa tgtgcatttt caggaattag
    13981 atctaatgat taaaatattg catatacatc ttagtagtaa aattagcgga gcccaaagag
    14041 tttctttaga tgaaatgaaa acgttgagta atcattactc acagtatatg gtttgctcca
    14101 aagaaggcga ttttactcaa gaagcggatc gtattggagt aaaaacttat gtaatagaaa
    14161 ccttagtcag agaaatttca cctctaaaag acttttattc attaataaaa ttatat
//
LOCUS       DAZGSP010000125        14182 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZGSP010000125 DAZGSP010000000
VERSION     DAZGSP010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.126"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..224)
                     /locus_tag="ACHK74_004321"
     CDS             complement(<1..224)
                     /locus_tag="ACHK74_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223033.1"
                     /translation="MNISYVNSNKTTSLPVELDALNNKDISYAKDFSYAKDFFLYIET
                     QLKIAKDFCRPGEEVSSSIASKVFHAFIDLV"
     gene            626..1339
                     /gene="aphA"
                     /locus_tag="ACHK74_004322"
     CDS             626..1339
                     /gene="aphA"
                     /locus_tag="ACHK74_004322"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFP0223034.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPVT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            1450..1866
                     /gene="yjbQ"
                     /locus_tag="ACHK74_004323"
     CDS             1450..1866
                     /gene="yjbQ"
                     /locus_tag="ACHK74_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFP0223035.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            1870..2226
                     /gene="yjbR"
                     /locus_tag="ACHK74_004324"
     CDS             1870..2226
                     /gene="yjbR"
                     /locus_tag="ACHK74_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP0223036.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(2261..5083)
                     /gene="uvrA"
                     /locus_tag="ACHK74_004325"
     CDS             complement(2261..5083)
                     /gene="uvrA"
                     /locus_tag="ACHK74_004325"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP0223037.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            5337..5873
                     /gene="ssb1"
                     /locus_tag="ACHK74_004326"
     CDS             5337..5873
                     /gene="ssb1"
                     /locus_tag="ACHK74_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFP0223038.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(5972..6253)
                     /gene="yjcB"
                     /locus_tag="ACHK74_004327"
     CDS             complement(5972..6253)
                     /gene="yjcB"
                     /locus_tag="ACHK74_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFP0223039.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            6683..8269
                     /gene="pdeC"
                     /locus_tag="ACHK74_004328"
                     /pseudo
     CDS             6683..8269
                     /gene="pdeC"
                     /locus_tag="ACHK74_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            complement(8272..8595)
                     /gene="soxS"
                     /locus_tag="ACHK74_004329"
     CDS             complement(8272..8595)
                     /gene="soxS"
                     /locus_tag="ACHK74_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFP0223040.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            8681..9145
                     /gene="soxR"
                     /locus_tag="ACHK74_004330"
     CDS             8681..9145
                     /gene="soxR"
                     /locus_tag="ACHK74_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFP0223041.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            9691..11040
                     /gene="ghxP"
                     /locus_tag="ACHK74_004331"
                     /gene_synonym="yjcD"
     CDS             9691..11040
                     /gene="ghxP"
                     /locus_tag="ACHK74_004331"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFP0223042.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            11192..12841
                     /gene="yjcE"
                     /locus_tag="ACHK74_004332"
     CDS             11192..12841
                     /gene="yjcE"
                     /locus_tag="ACHK74_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFP0223043.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(12995..>14182)
                     /locus_tag="ACHK74_004333"
     CDS             complement(12995..>14182)
                     /locus_tag="ACHK74_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP0223044.1"
                     /translation="YSKHHKNTLIDNKALSLFKMDDHEKVIGLIQKMKRIYDSLPSGK
                     ITKETDRKIHKHFIDIALYANNKCDDRITRRVYLSKEKEVSIKVVYFINNVAVHNNTI
                     EIPQTVNGGYDFSHLSLKGIVIKDEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIME
                     KILFDNCILDDSNFAQIKMADGTLNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAY
                     MAEVNLYKVIAPYVNLFKADLSFSKLDLINFEHADLSRVNLNKAILQSINLVDSKLFC
                     TWLTNTFLEMVICTGSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASV
                     QGMGISILRGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQ
                     HAD"
BASE COUNT         3410 a         3386 c         3598 g         3788 t
ORIGIN      
        1 actaaatcaa taaatgcgtg aaaaacttta cttgcaatag aacttgatac ttcttctcca
       61 ggtctacaaa aatcttttgc aatttttaac tgtgtttcta tgtataaaaa gaaatcttta
      121 gcataagaaa aatctttggc ataagagata tctttattat ttagtgcatc tagctcaaca
      181 ggtaaagatg tcgttttgtt gctattcaca tatgatatat tcatatcatc atctccatcg
      241 ggagtaataa aattagtcac cattgtagga tagggttctg gtcaatcagg aatcatcttt
      301 ataaacttcg attgtttttg taatgctgta tcattaagtt cattaaatcg tacagcagat
      361 aaatgttcta tcaaatttcg ctcatttgca gaggattcat cataataaac gtaaaattaa
      421 tgtatcctta catcgagtaa taaacatttt ttatacaaaa aaagacagga acgtatttac
      481 tgggttaaac ataatcatcc tgcttttcat cacaaaaacc gcagataatc cttcctttcc
      541 ccggcagctg gcgttatggt cagatggttt ttgcaacaaa tctcacaata aaaagtttca
      601 acatactgac tatttaggga aaaatatgcg caagatcaca caggcaatca gtgccgtttg
      661 cttattgttc gctctaaaca gttccgctgt tgccctggcc tcatctcctt caccgcttaa
      721 ccctgggact aacgttgcca ggcttgctga acaggcacca attcattggg tttcggtcgc
      781 acaaattgaa aatagcctcg cagggcgtcc gccaatggcg gtggggtttg atatcgatga
      841 cacggtactt ttttccagcc cgggcttctg gcgcggcaaa aaaaccttct caccagaaag
      901 cgaagattat ctgaaaaatc ctgtgttctg ggaaaaaatg aacaatggct gggatgaatt
      961 cagcattcca aaagaggtcg ctcgccagct gattgatatg catgtacgcc gcggtgacgc
     1021 gatcttcttt gtgactggtc gtagcccgac gaaaacagaa acggtttcaa aaacgctggc
     1081 ggataatttt catattcctg tcaccaacat gaatccggtg atctttgcgg gcgataaacc
     1141 agggcaaaat acaaaatcgc aatggctgca ggataaaaat atccgaattt tttatggcga
     1201 ttccgataac gatattaccg ccgcacgcga tgtcggcgct cgtggtatcc gcattctgcg
     1261 cgcctccaac tctacctaca aacccttgcc acaagcgggt gcgtttggtg aagaggtgat
     1321 cgtcaattca gaatactgac agagcgggag agcgtgacgc tctcccgaat gctgtttttt
     1381 taatcacacc tttatccttt cgctgtcttg ctgcaaactg attaagagag ttttatcaag
     1441 gagcagcaca tgtggtatca aaagacgctc acgcttagcg ccaaatctcg tgggtttcat
     1501 ctggtaacgg atgaaattct gaatcagctg gctgatatgc cgcgcgttaa catcggctta
     1561 ctgcatctgt tgctgcaaca tacctccgcc tctctgacac ttaatgagaa ctgcgatccc
     1621 accgtacgcc acgacatgga gcgttttttc ctccgcaccg ttcccgacaa cggaaattat
     1681 gagcatgact atgagggagc agacgatatg ccttctcata tcaaatcctc aatgctggga
     1741 acatcgcttg tattgccggt acataaaggg cgtattcaga ccggcacctg gcaaggcatc
     1801 tggctggggg aacatcgcat ccacggcgga tcgcgtcgca tcatcgcgac actacaaggg
     1861 gagtaaaaaa tgaccatttc ggagttgcta caatattgca tggcaaaacc aggcgcagaa
     1921 cagagcgtgc ataacgactg gaaagcgacg cagatcaaag tggaagatgt actgtttgcg
     1981 atggtgaaag aagtagaaaa tcgcccagct gtttcgctga aaaccagccc ggagctggcg
     2041 gagctgctac gtcagcagca cagcgatgtg cgtcctagcc gccatctgaa taaagcgcac
     2101 tggagcaccg tgtatctcga cggttcgctg ccggattcgc aaatctatta tctggtggat
     2161 gcgtcttatc agcaggcagt gaatttacta ccggaagaaa agcgtaaatt gctggtacaa
     2221 ctctgaaagg aaaaggccgc tcagaaagcg gccttaacga ttacagcatc ggcttgagga
     2281 agcgtgccgt atgcgaagct tcgcactccg cgacggtttc tggcgtaccg gagacgagga
     2341 tttcgccgcc gccactgccg ccttctggtc ccaggtcgac aatccagtca gcggttttga
     2401 tcacgtcgag attgtgctca atcaccacaa tggtgttgcc ctgatcgcgc agtttatgca
     2461 gtacgtcgag cagttgctga atatcggcga agtgcagacc ggtggtcggc tcgtcgagaa
     2521 tatacagcgt ctgcccggtg ccgcgttttg acagctcacg cgccagcttc acgcgctggg
     2581 cttcaccacc agaaagtgtg gttgcggact gccccaggcg aatgtacgtc aggccaacgt
     2641 ccatcaacgt ttgcagctta cgcgccagtg caggtacggc atcaaagaac tcacgcgcct
     2701 cttcgatggt catatccagc acttcgtgga tggttttgcc tttgtactta atttccagcg
     2761 tttcacggtt atagcgttta cctttacact gatcgcacgg cacgtagata tccggcagga
     2821 agtgcatctc tactttgatc acaccgtcgc cctgacaggc ttcgcagcgc ccgccacgga
     2881 cgttaaagct gaaacgtcct ggcgtataac cacgcgcacg ggattccggt acgcccgcaa
     2941 acagttcgcg cacaggcgta aacacgccgg tataggtcgc cgggttagaa cgtggagtac
     3001 gaccaattgg gctttggtcg atatcgatca ctttatcgaa atgctccagc ccctgaatat
     3061 cgcgatacgg tgctggttcg gcgatggtcg ccccattcaa ctggcgttgg gcaatcggga
     3121 acagtgtgtc gttaatcagc gtcgatttac cggaacctga aaccccggtg atgcaggtaa
     3181 acagacccac cggcagcgtc agcgtcacgt ccttcaggtt gttgccgcgt gcgcctgtca
     3241 gcttcagcac tttttccgga ttcgccggaa cgcgtttctt cggcacttca atcttgcgtt
     3301 tgccgctcat gtactgcccg gtcaacgact ccggcaccgc cataatcgct tccagcggac
     3361 cttctgcgac cacttcaccg ccgtgaacac ctgcgcccgg gccaatgtcg atcacatggt
     3421 cagcggcgcg aattgcgtct tcgtcgtgct ccaccacaat cacggtatta ccgagatcgc
     3481 gcagatggat aagcgtaccc aacaggcgct cgttatcgcg ctggtgcagg ccgatagatg
     3541 gctcgtccag cacgtacata acgccaacca ggcccgcacc aatctggctc gccagacgga
     3601 tacgctgggc ttcaccgccg gaaagcgttt ctgccgagcg ggaaagcgtc aggtaattca
     3661 ggccgacgtt aacgaggaat ttcaaacgat cgccgatctc tttaaggatt ttttccgcaa
     3721 tcttcgcccg ttgaccagcg agtttgagat tgttgaagaa ttccatcgca tgaccgatgc
     3781 tcatgtcgga gatagcaggc agcggcgtat tctcgacata aacgtggcgc gcttcccgac
     3841 gcagacgcgt cccttcgcag ctggcgcatg ggcgattgct gataaacttg gctaattctt
     3901 cacgtaccgc actggattct gtctctttat aacggcgctc catattgtgc agcacgcctt
     3961 cgaacggatg acgacggatg gaggtatcgc cacgatcgtt catgtatttg aattcaatgt
     4021 tttctttgcc agaaccgtac aacaccactt tatgcacgtt cgcgctcagg ctgccccacg
     4081 gcgcttcgac gtcgaactta tagtgatctg ccagcgattt cagcatctgg aagtaataga
     4141 agttgcggcg atcccagcca cggatcgcac cgccagccag tgacagctcg gggttttgga
     4201 tcacgcggtc aggatcgaaa tattgctgta cgccaaggcc gtcacaggtc gggcaggcac
     4261 ctgccgggtt gttaaacgaa aacagtcgcg gctccagttc acgcatactg tagccgcaaa
     4321 ttgggcaggc gaagttggcg gagaacagca gctcttccgc tttcgggtcg tccatatccg
     4381 ccactaccgc tgtaccaccg gaaagctcca gcgcggtttc aaacgactcc gcaagacgtt
     4441 gggtaagatc gtcacgcacc ttgaagcgat caaccaccac ttcaatggta tgtttctttt
     4501 gcagttccag tttcggcgga tcggaaagat cgcagacttc gccatcaata cgagcacgga
     4561 tgtaaccctg gcttgccagg ttctccagcg ttttggtgtg ttcgcctttg cgctctttaa
     4621 tgattggcgc gagcagcatc agacgcttgc cttccggctg cgacagcacg ttatccacca
     4681 tctggctgac ggtttgcgcc gccagcggga cgtcgtggtc cggacagcgc ggttcgccga
     4741 cgcgggcgaa caacaaacgc aaatagtcgt ggatttcggt gattgtcccc accgtagaac
     4801 gcgggttatg agacgtcgat ttctgctcaa ttgagatggc aggagaaagc ccctcaatat
     4861 gatcgacgtc cggcttttcc atcagtgaca gaaactgccg cgcgtaggcg gaaagggatt
     4921 caacgtaacg gcgctgccct tcggcatata aggtgtcgaa agcgagcgag gatttgccag
     4981 aacccgaaag cccggtcacg acaatgagct tgtcgcgggg gataacgagg ttgatgtttt
     5041 tgagattatg ggtgcgggcg ccccgaactt cgatcttatc cattcacctt tcccggatta
     5101 aacgcttttt tgcccggtgg catggtgcta ccggcgatca caaacggtta attatgacac
     5161 aaattgacct gaatgaatat acagtattgg aatgcattac ccggagtgtt gtgtaacaat
     5221 gtctggccag gtttgtttcc cggaaccgag gtcacaacat agtaaaagcg ctattggtaa
     5281 tggtacaatc gcgcgtttac acttattcag aacgattttt ttcaggagac acgaacatgg
     5341 ccagcagagg cgtaaacaag gttattctcg ttggtaatct gggtcaggac ccggaagtac
     5401 gctacatgcc aaatggtggc gcagttgcca acattacgct ggctacttcc gaatcctggc
     5461 gtgataaagc gaccggcgag atgaaagaac agactgaatg gcaccgcgtt gtgctgttcg
     5521 gcaaactggc agaagtggcg agcgaatatc tgcgtaaagg ttctcaggtt tatatcgaag
     5581 gtcagctgcg tacccgtaaa tggaccgatc aatccggtca ggatcgctac accacagaag
     5641 tcgtggtgaa cgttggcggc accatgcaga tgctgggtgg tcgtcagggt ggtggcgctc
     5701 cggcaggtgg caatatcggt ggtggtcagc cgcagggcgg ttggggtcag cctcagcagc
     5761 cgcagggtgg caatcagttc agcggcggcg cgcagtctcg cccgcagcag tccgctccgg
     5821 cagcgccgtc taacgagccg ccgatggact ttgatgatga cattccgttc tgatttgtca
     5881 ttaaaacaat aggttatatt gttttaaggt ggatgattaa agcatctgcc agccataaaa
     5941 aagaagcctc cgttatggag gcttctacgt atcaggtcaa aatcattggc cattgtggcg
     6001 gtgtctgatt catctcttct atcatcaccg actcgatgtt gtgccagata gcggtgatgt
     6061 ccgccattgt gatgccaagc aaccccattg cgaaccaaca ggcggaaaca acgcccagac
     6121 cgctgctgat caccgcaagc ccgacataat cagaccgacg aaaacggatg ttgagtgtgc
     6181 tggccagaaa catcattacg gcactaagaa gttgccagcg aagaagaacc acgccagtgg
     6241 tgagggtagc catcaaacaa ttcctctgaa aagagccgat gccctggaca gcgcggcgtt
     6301 gttcacggga ggcgtggtac actctggcta tcgcggggct tgcagaacac aaaaatgaaa
     6361 cactctgttt gtttcattaa ttttgtgaac tatatcacaa ttgattgttt gttagccata
     6421 ttaggccgtg acttttattg ctgtacagat tatgtggttt ttcagtggca ttaagggcat
     6481 atcttcccgc cgcctctgca ttcctgtagg aaattaattt tgaatatcaa tgaattattt
     6541 tcatccaggt gacgattaga aaggtatcaa tttcaaatca ggcaaaagtg ctatttatac
     6601 cgtaagattt atctaaagac gtcggtaccc agggttttca ccttgcaatg gccgggtata
     6661 aacaggcagg aaattgatag caatgagtca tcgtgcacga caccaattac tggcgttgcc
     6721 gggcattatc tttttagttc tctttcccat cattctttcg ctatggattg ccttcctttg
     6781 ggcaaaatca gaagtgaata atcagctccg aacctttgct caactggcac tggataaatc
     6841 cgagctggtc attcgccagg cagatttagt gagcgatgca gctgaacgct atcaggggca
     6901 agtttgcact ccagcccatc aaaagcgaat gttgaatatt attcgtggct atctttatat
     6961 taatgaattg atctatgccc gtgataacca ttttttatgc tcatcgctga tagcgcctgt
     7021 aaacggctat acgattgcac cggccgatta taagcgtgaa cctaacgttt ctatctatta
     7081 ttaccgcgat acgccttttt tctctggcta taaaatgacc tatatgcagc ggggaaatta
     7141 tgtggcggtt atcaaccctc tcttctggag tgaagtgatg tctgatgatc cgacattgca
     7201 atggggtgtg tatgatacgg tgacgaaaac ctttttctcg ttaagcaaag aggcctcggc
     7261 agcaacgttt tctccgctga ttcatttgaa ggatttaacc gtacaaagaa atggctattt
     7321 atatgcgaca gtttattcga caaaacgccc aattgcagcc attgttgcga cttcatatca
     7381 acgtcttata acccattttt ataatcatct tatttttgcg ttgcccgccg gtattttggg
     7441 gagtcttgtt ctgctattac tctggctacg tattcgacaa aactatttat ctcccaaacg
     7501 taaattgcaa cgcgccctcg aaaaacatta actttgtctt tattaccagc caataatcga
     7561 tatcaaaaca gaaaaatgta tcggcgctga agcgttgtta cgttggcttg gtgagcaggg
     7621 gcaaataatg aatccggcag agtttattcc gctggcagaa aaggagggga tgatagaaca
     7681 gataactgat tatgttattg ataatgtctt ccgtgatctg ggcgcgtact tggcaacaca
     7741 tgccgatcgc tatgtttcta ttaacctgtc ggcctccgat tttcatacgt cacggttgat
     7801 agcgcgaatc aatcagaaaa cagagcaata cgcggtgcgt ccgcagcaaa ttaaatttga
     7861 agtgactgag catgcatttc ttgatgtcga aaaaatgacg ccgattattc tggctttccg
     7921 ccaggctggt tacgaagtgg caattgatga ttttggtatt ggctactcta acttgcataa
     7981 ccttaaatca ttgaatgtcg atattttgaa aatcgacaaa tcgtttgttg aaacgctgac
     8041 cacccacaaa accagtcatt tgattgcgga acacatcatc gagctggcgc acagcctggg
     8101 gttaaaaacg atcgctgaag gcgtcgaaac tgaggagcag gttaactggc tgcgcaaacg
     8161 cggcgtgcgc tattgccagg gatggttttt tgcgaaggcg atgccgccgc aggtgtttat
     8221 gcaatggatg gagcaattac ccgcgcggga gttaacgcgc gggcaataaa actacaggcg
     8281 gtggcgataa tcgctgggag tgcgatcaaa ctgccgacgg aaaacgcggg agaaggtctg
     8341 ctgcgagaca taacccaggt ccattgcgat atcaaaaatc ggacgctcgg tggtgcgcaa
     8401 ctcaacggcg gccagtaaca ggcgacgttg gcgaatgtaa tcgccaagcg tctgatgcgt
     8461 caccgtgcgg aacattcgtt gcaagtacca ctttgaatag cctgattttt ttgcgactac
     8521 atcaatgtta agcggctggt caatatgctc gtcaatccat gcgataagat cctgaataat
     8581 tttctgatgg gacataaatc tgcctctttt cagtgttcag ttcgttaatt catctgttgg
     8641 ggagtataat tcctcaagtt aacttgaggt aaagcgattt atggaaaaga aattaccccg
     8701 cattaaagcg ctgctaaccc ccggcgaagt ggcgaaacgc agcggtgtgg cggtatcggc
     8761 gctgcatttc tatgaaagta aagggttgat taccagtatc cgtaacagcg gcaatcagcg
     8821 gcgatataaa cgtgatgtgt tgcgatatgt tgcaattatc aaaattgctc agcgtattgg
     8881 cattccgctg gcgaccattg gtgaagcgtt tggcgtgttg cccgaagggc atacgttaag
     8941 tgcgaaagag tggaaacagc tttcgtccca atggcgagaa gagttggatc ggcgcattca
     9001 taccttagta gcgctgcgtg acgaactgga cggatgtatt ggttgtggct gcctttcgcg
     9061 cagtgattgc ccgttgcgta acccgggcga ccgcttagga gaagaaggta ccggcgcacg
     9121 cttgctggaa gatgaacaaa actaaagcgc cacaagggcg ctttagtttg ttttccggtc
     9181 tttgtctttc tctctatccc gctggtacac aggagggttt cccccgacgt caacacaccc
     9241 cattcgagca cgtggtggag gttccggttg gtgttgatgc tttaattgta tgtcaccggc
     9301 atttcttcgc cagtgtaaaa gtatactttt tatccgcaat atttttgtca tctcagacga
     9361 ttttttatcg caatcctgaa cggtatacgg ctcgataacg ctgcaatctt gcgcaccgac
     9421 gataacgttt gcgcatcaat tgcctggttt ttcatcgtca agacaataaa agagaaaaaa
     9481 gcagcaaact tcggttgaaa aagccgctat gatcgccgga taatcgtttg ctttttttac
     9541 cacccgtttt gtatgcgcgg agctaaacgt ttgctttttt gcgacgcagt aaattgtcgc
     9601 aaacctggag caggaagata acgtttcgct ggcaggggat tgtccgccac gcatcttgac
     9661 gaaaattaaa ctctcagggg atgttttctt atgtctacgc catcagcgcg taccggcggt
     9721 tcactcgacg cctggtttaa aatttcacaa cgtggaagca ctgtccgtca ggaagtggtt
     9781 gccgggttaa caacgtttct ggcgatggtc tactcggtca tcgtcgttcc aggtatgttg
     9841 ggtaaagcag gcttcccgcc tgcggcagtt ttcgttgcaa cctgtctggt tgccggactc
     9901 ggttctatcg tgatgggtct gtgggctaat ctgccgttgg cgattggttg cgccatctcc
     9961 ctgacggcgt ttaccgcatt cagcctggtg ctggggcaac atattagcgt acctgtcgcg
    10021 ctgggtgccg tgttcctgat gggggtgctg tttacggtaa tttctgccac gggtatccgt
    10081 agctggattt tgcgcaactt gccgcacggt gtggcgcacg gcacggggat cggtatcggt
    10141 ctgttcctgc tgctcattgc cgctaatggt gtcggtctgg tgattaaaaa cccgcttgat
    10201 ggtctgcccg ttgcgctggg tgatttcgcg accttcccgg tgattatgtc actggtaggt
    10261 ctggcggtga tcatcggcct ggaaaaactg aaagtccctg gtggcattct gctgaccatt
    10321 atcggtatct caattgtcgg tttgatcttc gatcctaacg tccatttctc cggcgttttc
    10381 gccatgcctt cattgagcga tgaaaacggc aattcactga ttggcagcct ggacattatg
    10441 ggcgcgctga atcctgtagt cctgccaagc gttctggcgc tggtgatgac ggcagtattt
    10501 gatgccaccg gaactatccg tgccgtcgcc ggccaggcga acctgctgga taaagatggg
    10561 cagatcatcg acggtgggaa agcactgacc actgactcca tgagcagcgt tttctctggc
    10621 ctggtgggtg ctgctccggc agcggtatac atcgagtctg cggcgggtac ggcggcgggc
    10681 ggtaaaaccg gtttgacggc tatcaccgtt ggcgtgctgt tcctgctgat tctgttcctc
    10741 tctccgctct cttacctcgt tccggggtat gcaacggctc cggcgctgat gtacgttggc
    10801 ctgctgatgc tgagcaacgt ggcgaaaatc gactttgctg attttgttga tgcgatggcg
    10861 ggtctggtta cggcggtatt catcgtgctg acctgtaaca tcgtaacagg catcatgatc
    10921 ggcttcgcga ctctggtgat tggtcgtctg gtttccggcg aatggcgcaa gttgaacatc
    10981 ggtacggtcg ttatcgccgt ggcgctggtg accttctatg cgggtggctg ggctatctaa
    11041 tctttcatcg gattctgaaa acgggtggca atggctgccc gtttttattt tctccgcaca
    11101 ttgtgtgagc ttttgcgata ttctgaaaaa atgagaattc aggcataacg tctttctcag
    11161 atcataaaaa aacatcgcaa acagggaacg catggaaatc ttcttcacca tactgataat
    11221 gaccctcgtg gtctcgctgt ccggggtggt cactcgtgtc atgccctttc agatcccgct
    11281 tccgcttatg caaatcgcca tcggtgcgct actggcgtgg ccgacgtttg gtttgcatgt
    11341 ggagtttgat cctgaactct ttttagtctt gtttatcccg ccgttgctgt tcgctgatgg
    11401 ctggaaaacg ccgacccgtg aatttctcga acatggtcga gagattttcg gcctcgcgct
    11461 ggcgctggtg gtggtcaccg tggtcggcat tggcttcctt atttactggg tggtgccggg
    11521 cattccgctg atccccgcct ttgcgctggc ggcggtactt tctccgaccg atgctgtggc
    11581 gctctccggg attgttggcg aagggcgcat cccgaaaaaa atcatgggca ttttgcaggg
    11641 cgaagcgttg atgaatgacg cctccggcct ggtgtcgttg aagtttgccg tggcagtggc
    11701 gatggggacg atgatcttca ccgtcggcgg tgcgacggtc gaatttatga aagtagccat
    11761 tggtggtatt cttgccggtt ttgtggtgag ctggctgtat ggtcgctcgc tgcgattcct
    11821 cagccgctgg ggcggtgatg aacccgcgac gcagatcgtt ctgctgttct tgctgccatt
    11881 cgcttcttat ctgattgccg aacatattgg cgtttccggc atcctcgctg cggttgccgc
    11941 cgggatgacc atcacccgct ccggtgtgat gcgccgtgcg ccgctggcaa tgcgcctgcg
    12001 cgcaaacagc acctgggcga tgctggaatt tgtctttaac ggcatggtgt tcctgctgtt
    12061 aggtctgcag ctgccgggta ttctggagac gtcgctgatg gcggcagaaa tcgaccctaa
    12121 cgtcgaaatc tggatgctgt ttaccgatat tattctgata tatgcggcgc tgatgctggt
    12181 ccgtttcggc tggctgtgga cgatgaaaaa gttcagcaac cgcttcctga agaagaagcc
    12241 gatggagttt ggttcgtgga ccacacgaga aatcctgatc gcgtctttcg ccggggtgcg
    12301 tggggcgatc actctggccg gtgtgctctc tatcccgctg ctcttgccgg atggtaacgt
    12361 cttcccggcg cgctatgagc tggtgttcct ggcggctggc gtcattctct tctcgctgtt
    12421 tgtcggcgtg gtgatgttgc ctattctgct acaacacatt gaagtcgcgg accattcgca
    12481 acaattgaaa gaagaacgta ttgcgcgagc ggcaacggca gaagtggcga ttgtggcgat
    12541 ccagaaaatg gaggagcgtc tggcggcgga taccgaagag aatatcgata accagctgct
    12601 tacggaggtc agttctcgcg tcattggtaa cctgcgtcgt cgcgccgatg gacgtaacga
    12661 cgttgaaagt tccgtgcagg aagagaacct tgagcgtcgc ttccgtctgg cggcattgcg
    12721 ttctgaacgt gctgaactgt accacctgcg cgccacgcgg gagatcagca acgaaacgct
    12781 gcaaaaatta ctgcacgatc tcgatttgct tgaagcgttg ctaattgagg aaaatcagta
    12841 agccgaaagc gacgtaacca ggcccgaggt aatctgaaaa aggtgcaatc ttttgcacct
    12901 ttaacaggtt agctcgggct aacattcatg attctaaaac aaataagtta tctctcttgt
    12961 aaagtcaatt cattggctgt tcttcgactt aacctcaatc tgcgtgttgc ataatatctg
    13021 cagtaatctt catagcgact gcattaatat tatcctcagt ttgtgacatg ccagtatgag
    13081 aggtacaatc ttcttcaaag aatttctgta gtgttaccag ggtatcacta tcaatgggga
    13141 tattttcctc cccacggaga atagaaatgc ccattccctg gacgctagcc tcatcaaaat
    13201 taacccgata gagacgggta ttaaacatac aggcttttgt taaaatagaa cagttgaaat
    13261 ggcagttgct taaattggca ttattaaaat taacattagc catattagag ccggtacata
    13321 taaccatttc gaggaatgta tttgttagcc acgtacaaaa gagtttgcta tcaactaagt
    13381 ttatactctg gaggattgct ttgttcagat tgacgcgaga cagatcagca tgttcaaagt
    13441 taattaaatc gagtttagag aaactaaggt cggctttaaa taaattaaca tatggcgcta
    13501 ttactttata aagatttacc tcagccatgt acgccatata aaaatttgaa tagtcaagaa
    13561 aggtattttt aatattggct ctattcattg ctgcattgta gaattgaaca tgcatagcgg
    13621 agcatgcatt tagagttccg tcggccattt ttatctgagc gaaatttgag tcatcgagaa
    13681 tacaattatc aaaaagtatt ttttccatta tggcgtaata aaaattcgtt ttatacatat
    13741 tacagtcctg gaaaatagcg ttttgtagtc tgcaacctgc aaaattcgaa ttggataaat
    13801 cttcatcttt aatcacgata cctttcaggc taaggtgtga aaaatcgtaa ccaccattta
    13861 ctgtctgtgg aatttcgata gtattattat ggacggcgac attatttata aaatatacca
    13921 ccttaatgga tacttccttt tctttactaa ggtaaactct tctcgtaatt ctatcgtcac
    13981 atttattatt tgcatataaa gctatatcta taaaatgttt atgtattttc ctgtccgttt
    14041 ccttcgtgat ttttcctgat ggtaaactat cataaattct tttcattttc tgaatcaaac
    14101 ctatcacttt ttcatgatca tccatcttga aaagagagag ggctttattg tcaattaacg
    14161 tatttttatg atgtttgctg ta
//
LOCUS       DAZGSP010000126        14153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZGSP010000126 DAZGSP010000000
VERSION     DAZGSP010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.127"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..643)
                     /locus_tag="ACHK74_004334"
     CDS             complement(<1..643)
                     /locus_tag="ACHK74_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFP0223045.1"
                     /translation="MSVMHYLQDKTFQDIVKNLVLPLLSGIIIPLVKWFVQHYGYAPN
                     IRKYRFEKIPVSEKESILARIDKLTKEPTTKNTLVRIKYCYEQMGIYLPIWCCNKLIC
                     FISDRNVSSVDNRLHCFLKYSFVGMFSDGKFTVNTRRVHKGYRMIAVFAVFSLCVQFT
                     GGIFTTMPFLSGGNTVLFMLFSLVYFIMIFLTVIFTCNLINEIRLAVQFGRLFE"
     gene            complement(645..2243)
                     /gene="tssA"
                     /locus_tag="ACHK74_004335"
     CDS             complement(645..2243)
                     /gene="tssA"
                     /locus_tag="ACHK74_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFP0223046.1"
                     /translation="MASIHSLLSACQTTPRDVAEPAQVRIALWDKWLAPVTPDNPAGD
                     DAGYDDDFQQMREEVNKLSGADAGIVSQLAEKLLTTRTKDIRVATWYIWARLRQDGEK
                     GLADGLELLTGLLQRFGEHLHPQRSRARKAALEWLCSARILDSLSLYPEVVKADTLRI
                     AGALWLAEQTFTDEASAPVLNGLYQALENRLMKAGGVDAVVPQEAAAPAPTVTSGSVM
                     ALSAITSGQELLSQARVLAKYLRDQPEGWLAAHRLMKSVRHDTLHQLPPLSADGRTRI
                     APPGPDRRASLKRLYLQQNWLSLLEQCDDMFARGASHLWLDLQWYIHQALLQTGKENY
                     AAIIQYDLKGLLLRLPGLETLAFNDGMPFADDVTLSWIQQQVMECGERWAEEPSVTIT
                     AAPGDNDILSLEPEALQIADNEGTEAALSWLQARPGIQSDRSNWLLRLLMARVAEQTG
                     KNDLALHLLVELDERATRLTLSQWEPELVFEVKARRLKLLRMKSAKTESDRVRLQPDM
                     EHLLAGLIAIDAARAAVLCNPGSS"
     gene            complement(2243..5632)
                     /locus_tag="ACHK74_004336"
     CDS             complement(2243..5632)
                     /locus_tag="ACHK74_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFP0223047.1"
                     /translation="MNKLACLSGRFGRPGIVFIGVAALWWLITRYGAFLGAETRRDQI
                     LLLILLSLGLLFVCYLPVMKKYVQELTYRRRARKEQRLPDDEERLAQTPPRYVTVQDI
                     RHTLRRQYGRFWGRKIRILLITGTASEVELLTPGLTEQFWQEEQGTLLLWGGEPSQPE
                     NADWLAALRRLRYRPADGIVWVTSGLSETLSAPLTEDALDRVSRAVSSCCERLGWRLP
                     LYVWSLQESPDERGRITQPVGCLLPAECSSDKLKAQLQAMLPGLVAQGIQQICCAPRY
                     YFLLSLAERFRRNIDAVVEPLSVLLRPYRQLLLAGIVFSPATVGGERSVRHRWRMDNR
                     WEALPETVQQLPVRLQPSRTGHNWRRSLAVMAAILMMAQGTGMVVSFLANRSLVAEVQ
                     EQIRPAQNQQLSPAERLQALLNLQKSLARLQYREEHGAPWYLRAGMNQNADLLAVVMP
                     LYAQNAHLLLRDAAAAHLEQQLRTFIRLPPDSPQRGKMAKAAYDQLRLYLMLTQPQHM
                     EPAWFSRTLMREWPQRDGVSAVFWQANGPTLLAYYASGIITHPQWKLTADEELVSQSR
                     TLLLRHLGTQNSDAMLYQKMLARVAHQFADMRLTDMTGDTDVSRLFFTDEVVPGMFTR
                     QAWEEAVLPSIDTVINERREEMDWVLTDGRQKAPSPVSPEALRQRLTTRYFADFGNAW
                     LNFLNSLHLRKAQTLSDVTEQLTLMADVRQSPLVALMNTLAVQGRTGQPREAVTDSLV
                     KSARNLLSQEKQPVAVPESRLHGPLATTFGPVLALMDNQNNSADMLNLQTYLTRVTQV
                     RLRLQQIAGSSDPQAMMQMLAQTVLQGKSVDLTDTRDYGSLTAAGLGQEWYGFGQTVF
                     VRPMEQAWQQVLTPAAESLNARWRTAVVDGWNNAFSGRYPFKNVSSDASLPLLAKYLN
                     TDTGRIARFLQNNLSGVLHSEGSRWVPDTINTRGLTFNPAFLKAINTLSEIADVAFTT
                     GNAGLHFELRPGTAAGVMQTTLITDNQKLIYVNQMPVWKRFTWPADTEAPGASLSWVS
                     TQAGTRQYADLPGSWGLIRLLEMARRKAASGVASGWSLSWQAQDGRMLNYTLRTEAGE
                     GPLVLLKLRNFVLPETVFELSGTSAFTGNDEDAGDSVEETD"
     gene            complement(5625..6773)
                     /locus_tag="ACHK74_004337"
     CDS             complement(5625..6773)
                     /locus_tag="ACHK74_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFP0223048.1"
                     /translation="MGWSRTKSLSTELPHKPSMMLWFMTGAFMAVVGALLFIIRASEY
                     VKALNDFSIWWLALTPPGGWFFLFCLRHWQWSNQMDEHLFLKKEGEYAQKQWESWAER
                     YLVITASCVYLPDKITVATLCDELPLQYGLVKKIDYLSDSGHKVEASLRVLLREITDK
                     FCQLPAVLPVNVTLITDQPDSEIRSAFVSAWEVLFPQRVVPDNIEVTPDFSMGWVDER
                     LKQPVLTVDLILVIQLNGGNAYSDGLAALLLTSDDVAQKYNLPHPARLLRPMSLDINK
                     FNDEFTLFLETQTAACRTARVLGDCYHWEKIAAPLMTIGNQYGAGWEPSGRMLLEKWC
                     GIPGPAAPWLLTALAADLVCLGNDSLLTLFSSGEEHFISTVTSGNQNE"
     gene            complement(6777..7043)
                     /locus_tag="ACHK74_004338"
     CDS             complement(6777..7043)
                     /locus_tag="ACHK74_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFP0223049.1"
                     /translation="MSKGFVLLGDNTTHGGKVISASSTMIVDGKKVALVGDMVSCPVS
                     GHGTNPIVEGSPEWISDGRAVVIDGCKCLCGCKVISSAPECAIG"
     gene            complement(7074..7751)
                     /gene="tli1"
                     /locus_tag="ACHK74_004339"
     CDS             complement(7074..7751)
                     /gene="tli1"
                     /locus_tag="ACHK74_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity phospholipase A1-binding
                     lipoprotein Tli1-EAEC"
                     /protein_id="HFP0223050.1"
                     /translation="MTRDKIIFIAPLLALLTGCNSVSSQYADSDKTEWGYKKLPYDEW
                     KFLFIYPKALPALATQALMVDGEGYKTTYQYLDNTEPSQVSVGRWNDHLGATESYYNK
                     GKALPLMLRFCWDSIIDKKSYETLIFFKKGTWEQMVTPYTDNRWNETYYRSTMIIGLA
                     PEGKVRVWLGDRGNPVVPQLDAKITTVSGDKMNMCKGVTKHPDGYVYYGDTPDFIKGK
                     IYPYGNW"
     gene            complement(7771..9453)
                     /gene="tle1"
                     /locus_tag="ACHK74_004340"
     CDS             complement(7771..9453)
                     /gene="tle1"
                     /locus_tag="ACHK74_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector phospholipase Tle1-EAEC"
                     /protein_id="HFP0223051.1"
                     /translation="MTKYQGYDVTDATHKTSIHNDWKVVVAKKKPARGVTLTIGIFFD
                     GTGNNRENTASRLMKFNECSAARQGVNQKDAQSCEDFLEEINKNNISNGSYRGYYSNI
                     HWLNILYHPDQVLKKDQTSAQIKTYISGIGTAAGEADSVIGMGLGTSILDIFEGVVTK
                     TDEAMERITQALSEFMGFNLNPDFCIAKIQFDVFGFSRGAAAARHFANRVMEQDPAIA
                     RAIAKGLRGDFYDGKPSGEVRFLGLFDTVAAIGGISNFFDINGRSNPGVKLELRPSIA
                     KKVFQITAMNEYRYNFSLNSIKGMWPELALPGAHSDIGGGYNPVGSPLQENESLFLSC
                     PAFEIVSDDTRETDTRVYRNAEQARKMLMTLPALKHILPHGKLTTKIRSVGVNNSNQR
                     RAGVIQKQVGAAVFFERMAVPNDWANVCLRVMLDAAQEAGVLFAPIDPKNPDMNLSPE
                     LIPFVDKAIAQGKAVRLGKEPQAFTEEELYIIGKYTHCSANWNIESDGNLWVDPTTGE
                     IFIHRFGPKGNKAFVFPNKPNDRWIRSVWYMDDQQRLNDNAVKNTKVMMSGV"
     gene            complement(9450..11969)
                     /locus_tag="ACHK74_004341"
     CDS             complement(9450..11969)
                     /locus_tag="ACHK74_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP0223052.1"
                     /translation="MSLTDTLSKTISASLNRYHLIIPSCPSPLDVEHFSGREEMSQTY
                     YYIINFTSTDNDLDAGLLLRKSATFTMGTGGLKEQVSQKVVHGVITDFRRISGSADQA
                     KYQVILEPFIKLLDRQHRSHRFFVNKSVPEVVTEVLQEHGLKGWEFEFRLKKTYPKRE
                     QINQYQESDLQFIQRLLAEVGIFYFFTLQPDTQTEIVHFGDSQAALTFDKTLAINSPS
                     GMNDNRADSVWGLNVTHNVAEASVTTKAYNHREAQYLLQSAPADMTHGDGDGINYGEV
                     YHYHFRHLERGDKIDPVPETANFYARLGHERYLAEQVRITGNSTDATLAPAQVLTITD
                     SLPPTLPALLRNPVLLTCVGFSASRKDALQVVLKGVPYSEVICWRPPLLPRPKITGTM
                     TARVTSAKEGDIYAWQDASGMYRVKFDADRDDKNPGQESMPVRLAKPYSGDAYGFHFP
                     LIQGTEVAIAFEEGDPDRPYIAHALHDSRHVDHVTDKNGTRNVIRTPANNKLRMEDKR
                     GEEHIKISTEFGGKTQLNLGHNVNASRKLRGEGAELRTDDWISIRGGKGIFISADMQP
                     QAQGKMLDMNEAIRQLEQALSLARSMAKAATAANATQGDISCQQRLNSSLTDLTAPGM
                     LLHAPDGIGMVSARALRIASGSESVGIMSGDNTDISAGQSFTVVAEGAVSLLSRNQGM
                     QLLAAKGRVNIQAQSDDLSMSSQQNLDIQSSEGKVTVSANQELILACGGAYIKLSGGN
                     IELGCPGQILLKSTNMQKMGPTSLDIASVEMPRGFGGGFILTDEAGVPQPSTPYRLTT
                     AEGDILQGITDENGKTAPVNTSIPSVVKVEFGKVKIHGETE"
     gene            complement(12272..12913)
                     /locus_tag="ACHK74_004342"
     CDS             complement(12272..12913)
                     /locus_tag="ACHK74_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223053.1"
                     /translation="MDSAKEIARDMLEAVNINGDGFLGAVGKGLISLPVSLGYMGYDF
                     IDTGHRRENQNDKFRMARLVEKGIFSRDIIGKIISICLEDFVSRINMEKTGSIVKNVS
                     GDLAGRMAFAELTGVKLGEVLVSRGLAAFFAGSFAGLLLSVGAETSRAIYTSRFLKDR
                     NPVLHDKLKAQGDLDLLYFLVEDIVRPFERACIVADRNSAEFDRICEYFFGGL"
     gene            complement(12900..>14153)
                     /locus_tag="ACHK74_004343"
     CDS             complement(12900..>14153)
                     /locus_tag="ACHK74_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0223054.1"
                     /translation="GERLSAIEQEENELMTARDDLEQQYARECELTGELLESRSDISR
                     QSETLHLQQALHDIQQNQPLLSVDVDVRTVAGVVADWTGVPLSSLMKDEQMELLHLEK
                     DIGRRVVGQDVALESIAQRLRAAKTGLTSGNGPQGVFLLVGPSGVGKTETALALADVM
                     YGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGILTEAVRKRPYSVVLLDEVE
                     KAHRDVLNLFYQVFDRGFMRDGEGREIDFRNTVILMTSNLGSDLLMQQLSEKPETTES
                     ELHELIRPLLRDHFQPALLARFQTVIYRPLTQSAMRIIVEMKLAQVCERLHCHYGLST
                     SVDERVFDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSYLAARQHPTYLSLTWN
                     DEDGIVIAFDGINSDYPQESIYGQC"
BASE COUNT         3654 a         3926 c         3062 g         3511 t
ORIGIN      
        1 cctcaaacaa tcgcccgaac tgaacggcaa gccggatttc attaatcaga ttacatgtga
       61 atataactgt cagaaatatc atgataaaat aaacgagcga aaataacata aataaaactg
      121 tattaccccc cgacagaaat ggcatggttg taaatatccc tcccgtaaac tgaacacata
      181 aagaaaacac cgcaaacact gctatcatcc ggtaaccttt atgcacacga cgagtattaa
      241 cagtaaattt gccatctgaa aacataccga caaaagaata tttaagaaaa caatgcagtc
      301 tgttatccac tgaactgaca tttctgtcgg atataaaaca aatcagctta ttacaacacc
      361 atatcggaag atatatcccc atctgttcat aacagtattt tattcttacc agtgtatttt
      421 tcgttgttgg ctcttttgtc agcttgtcaa tacgggcaag aatggactcc ttttcagata
      481 caggaatttt ctcaaatctg tatttcctga tattcggcgc atagccataa tgctgcacaa
      541 accatttgac aagaggaata ataatgcccg acagaagcgg taacaccaga tttttaacta
      601 tatcctgaaa tgttttgtcc tgaagataat gcatgacaga catctcatga gctccccgga
      661 ttgcacaaca cagcagctct ggcagcatca atggcgatga gcccggcaag aagatgttcc
      721 atatcaggct gcagacgcac cctgtcactt tcagtttttg cggacttcat acgtaacagt
      781 ttcaggcggc gggcttttac ctcaaatacc agttcaggtt cccattgtga cagggtcagc
      841 cgggttgcac gttcatccag ctccacaagc agatgcaggg caaggtcatt ttttccagtc
      901 tgttcggcaa cacgggccat caacaggcgc agtagccagt tgctgcggtc agactgaata
      961 ccgggacgtg cctgtaacca gcttaaagcg gcttctgttc cttcattatc cgcaatctgc
     1021 agggcttccg gctcaagaga taaaatgtcg ttatctccgg gagctgccgt tatcgttaca
     1081 gaaggttctt ccgcccagcg ttcaccacac tccatcacct gctgctgtat ccagcttagc
     1141 gtcacgtcat cggcaaacgg catgccgtcg ttaaaagcca gtgtctcaag ccccggcagg
     1201 cgcagaagca atcctttcag gtcatactga ataatggcag cataattttc ttttccggtc
     1261 tgcaacagcg cctgatgaat ataccactgc aggtccagcc acagatgact ggctccccgg
     1321 gcaaacatat cgtcacactg ttcaagcagt gacaaccagt tctgctgcag atacaaccgt
     1381 ttcagtgacg cacgccggtc cggccccggt ggggcaatac gggtccgtcc atcagcagat
     1441 aacggcggta actgatgcag cgtgtcatgc cggacgcttt tcatcagtcg atgagccgcc
     1501 agccagccct caggctggtc acgcagatat tttgccagaa cacgggcctg tgacagcagt
     1561 tcctgtccgg aggtgatggc agataatgcc atgacgctgc cggatgttac cgtcggtgcc
     1621 ggcgcggcag cctcctgagg caccactgca tcaacaccac cggccttcat caggcgattt
     1681 tcaagtgcct gatataaccc gttcagcacc ggggcggaag cctcatccgt aaaggtctgt
     1741 tctgccagcc acagcgcccc ggcaatgcgc agggtatccg ccttcaccac ctccggatac
     1801 agagacagac tgtccagtat gcgggcgctg cataaccact ccagcgccgc cttcctggcc
     1861 cgtgaccgtt gtggatgcag gtgctctcca aaacgctgca acaaaccggt cagcagctcc
     1921 agaccgtcgg ccagcccttt ttcaccatcc tgccgcagac gggcccagat ataccaggtc
     1981 gccacccgga tatctttggt tctggtggtc agtaattttt ctgcaagctg gctgacaatt
     2041 ccggcatcgg ctccggagag tttattgacc tcttcacgca tctgctgaaa atcatcatcg
     2101 taccccgcat catcacctgc gggattatct ggcgtcacgg gagcaagcca tttatcccac
     2161 agcgcaatac gcacctgagc cggttcagcc acatcccggg gtgtcgtctg gcatgcactg
     2221 aggagcgaat gaatggaagc catcagtcag tctcctccac ggaatccccg gcatcttcgt
     2281 cattgcctgt aaacgctgac gtaccggaga gttcgaaaac cgtttccggc agaacaaaat
     2341 tgcggagttt cagcaataca agcggccctt cccccgcttc agttcgcagt gtgtaattca
     2401 gcatacgacc gtcctgcgcc tgccagctca ggctccagcc actggcaacg cccgatgcgg
     2461 ctttccgtcg ggccatctca agcagacgta tcagccccca gcttcccggc aggtctgcat
     2521 actgacgggt accggcctga gtgcttaccc agcttaaact ggcgccaggt gcttccgtat
     2581 cagccggcca ggtaaatcgc ttccataccg gcatctgatt aacataaatc agtttctgat
     2641 tatcggttat cagcgtcgtc tgcatcacac cggcagcagt tcccgggcgc agctcaaaat
     2701 gcagccccgc gttacctgtg gtgaacgcca catccgctat ttcactcagg gtattgatgg
     2761 ctttcagaaa cgcagggttg aatgtcagtc cccgggtatt aatggtatca ggtacccagc
     2821 gactgccttc actgtgcaac acaccactca ggttattctg caggaaacgg gcaatacgtc
     2881 ccgtgtccgt attcaggtat tttgccagca ggggaagcga agcatcgctg ctgacatttt
     2941 taaacggata acgcccggaa aatgcattat tccagccatc caccaccgca gtacgccacc
     3001 gggcattcag actttctgcc gcgggcgtaa gaacctgctg ccacgcctgc tccatcgggc
     3061 gaacaaagac cgtctgtccg aagccatacc actcctgtcc gagtccggca gcggtcaggc
     3121 taccgtaatc ccgggtatcg gttaaatcaa ccgatttccc ctgcaatact gtctgtgcca
     3181 gcatctgcat catggcctgc gggtcagaag agccggcaat ctgttgcaga cgcagacgaa
     3241 cctgtgtcac ccgggtcaga tatgtctgca gattcagcat atccgcactg ttgttctgat
     3301 tatccatcag tgcaagcacc ggcccgaaag tcgtcgccag aggaccgtga agccggcttt
     3361 ccggtactgc cactggctgt ttttcctgtg aaagcaggtt tctggcagat ttaaccagtg
     3421 aatcagtaac cgcttcccgg ggctgaccag tacgcccctg aaccgccagc gtattcatca
     3481 gtgcaaccag gggggactgg cgcacatcag ccatcagcgt cagttgctct gtcacatcag
     3541 acagcgtctg agctttacgc aagtgcagac tgttcagaaa attcagccag gcattaccaa
     3601 aatcagcaaa atagcgcgtt gtcaggcgct gtctgagtgc ttccggcgat accggtgacg
     3661 gtgccttctg cctgccatcc gtcagcaccc agtccatttc ttcacgacgc tcattaatca
     3721 cggtgtctat cgacggcaat acggcctctt cccaggcctg gcgggtaaac ataccgggaa
     3781 ccacttcatc cgtaaaaaag aggcgggaga catcagtatc accggtcata tccgtcagac
     3841 gcatatccgc aaactgatgt gccacccgtg ccagcatttt ctgatacagc atggcatcgc
     3901 tgttctgtgt tcccagatgg cgaagcagca gagttctgct ctggctgacc agctcttcat
     3961 cagccgtcag cttccactga ggatgagtaa taatcccgga ggcataatac gccagcaacg
     4021 tcgggccatt agcctgccag aaaacggcac tcacaccgtc acgctgtggc cattcccgca
     4081 tgagcgtacg actgaaccag gcgggctcca tatgttgcgg ttgcgtcagc atcagataca
     4141 gtctgagctg gtcatatgcg gcttttgcca ttttcccccg ctgcgggctg tccggaggaa
     4201 gacgaataaa tgtcctgagc tgctgctcaa gatgagcagc agcggcatca cgcagtaaaa
     4261 gatgtgcatt ttgtgcataa aggggcatta cgaccgcgag caggtctgca ttctgattca
     4321 taccggcccg caggtaccat ggcgccccgt gctcctcccg gtactgaagg cgggccaggg
     4381 atttctgcag attcagcaac gcctgcagac gttctgcagg agacagttgc tggttctgag
     4441 ccggacgaat ctgttcctgt acttcagcaa ccagactccg gtttgccaga aaggaaacca
     4501 ccattcctgt tccctgagcc atcatcagta tggccgccat aacggccaga cttctgcgcc
     4561 agttatgacc tgtgcgggaa ggctgtagcc ggacaggtaa ttgctgcact gtctccggca
     4621 acgcctccca gcggttatcc attcgccagc gatgtctgac acttcgttca ccgccgacag
     4681 ttgccggact gaaaacaata cccgccagca acaactgacg gtatggacgg agtaatacgg
     4741 ataatggctc caccacagca tcaatatttc gtctgaatcg ctctgccagc gacaacaaaa
     4801 aataataccg aggggcacag catatctgct gtatcccctg agcaacaagc ccggggagca
     4861 tggcctgtag ttgtgctttc agtttgtcag aactacattc tgctggcaga aggcatccca
     4921 cgggctgagt aatacgccct cgctcatccg gactctcctg cagtgaccag acatacagcg
     4981 gcagacgcca gccaaggcgc tcacaacatg aactgaccgc acgggaaacc ctgtcaaggg
     5041 catcttctgt cagtggtgcg gataatgttt cactcagtcc ggaagttacc cagacaatgc
     5101 catcagcagg ccggtaacgc agacggcgta acgcagccag ccagtctgcg ttttccggct
     5161 gcgagggctc tcctccccag agaagcaatg tcccctgctc ttcctgccag aactgttctg
     5221 tcaggcccgg ggtcagcaac tcaacctcag aggcagtacc tgttatcaac agaatgcgta
     5281 ttttacgtcc ccagaatctg ccatactggc gccgcagagt atgacgaata tcctgaacag
     5341 taacatagcg cggtggcgtc tgagccagtc gctcttcatc gtcaggtaac ctttgctcct
     5401 tacgggcccg acgtcgatat gtcagttcct gaacatattt tttcatcaca ggcaggtaac
     5461 agacaaagag caaccccaga ctcagcaata tcagcagtag tatttgatcc cggcgtgttt
     5521 ctgcccccag aaatgctcca tatcgggtta tcagccacca gagcgcagcc acaccaataa
     5581 atacaatccc cggtcgtcca aagcgaccag acagacaggc cagtttattc attctggttt
     5641 cctgatgtaa cagtactgat aaaatgttct tctccggatg agaataaagt caggagagaa
     5701 tcattaccaa gacagaccag atcggctgcc agagcagtaa gcagccacgg agcagcaggt
     5761 cccgggatac cgcaccattt ttccagtaac atacgtccgg acggttccca tccggcacca
     5821 tactggtttc caatagtcat cagcggtgca gcaatctttt cccagtgata acagtccccc
     5881 agtacacgag cggtacgaca ggcagctgtc tgggtttcaa ggaaaagtgt gaattcatca
     5941 ttaaacttgt tgatatccag cgacatcggg cgtagcagac gggctggatg cggcaggtta
     6001 tatttttgcg caacatcatc gctggtcaac aaaagtgcag ccagtccgtc agagtatgca
     6061 tttcccccat taagctgtat taccagtatc aaatccacag tcagcactgg ttgtttcaga
     6121 cgctcatcaa cccaccccat ggaaaagtcg ggagtaacct cgatattgtc aggaacaaca
     6181 cgttggggaa ataacacttc ccatgcagaa acaaaagcag atctgatttc agaatcaggc
     6241 tgatcggtta tcagcgtcac atttacaggg agaacggcag gtaactgaca aaatttatcc
     6301 gttatctccc taagcaagac acgtaaagag gcttcaacct tatgcccact gtcagaaaga
     6361 taatctattt tctttacaag gccgtactgc agaggcaatt catcacaaag agtagctacc
     6421 gttattttat caggcaaata aacacaactg gcagtaataa caagatacct ttcggcccag
     6481 gattcccact gtttctgagc atactccccc tcttttttaa gaaacaaatg ttcatccatc
     6541 tggttgctcc actgccagtg tcgcaggcaa aacaggaaaa accagccccc cggaggagtc
     6601 aatgcaagcc accagatact gaaatcattt aatgctttca catattctga tgcccggata
     6661 ataaacaaca gagcaccaac aacagccata aatgcccctg tcataaacca caacatcatt
     6721 gatggtttat gaggtaattc tgttgataat gattttgttc tgctccatcc catgggttat
     6781 cctattgcac attccggtgc actggatata accttacacc cacacagaca tttacatcca
     6841 tcaatcacaa cagctcttcc atcagatatc cattcaggag agccctccac aataggatta
     6901 gtaccatgtc cggaaacagg acaactaacc atatctccga caagagctac ttttttacca
     6961 tcgacaatca tggttgaaga tgccgaaata actttaccac catgtgtggt attatcacca
     7021 agcaatacaa aacctttaga cataaaactc tcaaaaataa attttacttt gagtcaccag
     7081 ttaccgtagg ggtaaatttt ccctttgata aaatctggtg tatcaccgta ataaacatat
     7141 ccatctggat gtttagtaac ccccttacac atattcattt tatcacccga tactgtagta
     7201 attttagcat ccaactgagg taccaccgga ttaccgcgat ctcccagcca gactcttacc
     7261 ttcccttcag gagccagacc aataatcatc gttgaacgat aataggtttc gttccagcgg
     7321 ttgtctgtgt aaggcgtaac catctgctcc caggtccctt ttttaaaaaa aatcagagtt
     7381 tcataactct ttttatcaat tattgaatcc cagcaaaaac gtaacatcag aggtaatgct
     7441 ttccccttgt tataatacga ctcagtcgcg cccagatggt cattccagcg ccctacactg
     7501 acctgactcg gctcggtatt atcaagatac tgataagtcg tcttataacc ttccccgtcc
     7561 accatcaatg cctgagtggc cagcgcaggc agagcctttg gataaataaa gagaaatttc
     7621 cattcatcat agggtaattt tttataaccc cattcagtct tatccgaatc agcatactgt
     7681 gagctaacag aattacaccc agttaacaat gccaataatg gtgcgataaa aatgatttta
     7741 tctctggtca tgtattgtca tcctgtagcg ttaaacacca gacatcataa ctttagtatt
     7801 ttttactgcg ttatcattca atcgctgctg atcatccatg taccatacag aacgaatcca
     7861 gcgatcattt ggtttattcg gaaaaacgaa cgccttatta cctttaggac caaagcgatg
     7921 gataaaaatc tctcccgttg taggatccac ccataaatta ccatcagact caatattcca
     7981 gttggcagaa caatgggtgt attttccgat aatatacaac tcttcctcag taaaagcctg
     8041 aggctctttc cccaaacgta ccgcttttcc ctgagcaatg gctttatcaa caaaaggaat
     8101 taattcagga gataaattca tatctggatt tttggggtca attggagcaa acaatacccc
     8161 tgcttcctga gcagcatcca gcatcacacg caggcaaaca ttagcccagt cattaggtac
     8221 tgccatacgc tcaaaaaata ctgcggctcc cacctgcttt tgtatcacgc cagcccgacg
     8281 ttgattgctg ttgttcacac ctacggaacg aattttggtg gttagttttc catgaggcaa
     8341 aatatgtttt aaggctggca gagtcatcaa catttttctg gcttgctctg cattacggta
     8401 tactcgcgta tccgtttctc tggtatcgtc actgacaatt tcaaatgccg ggcaactcag
     8461 aaacagactt tcattttcct gcagaggtga acctaccgga ttgtaaccac ctccaatatc
     8521 tgaatgcgcc cctggtaaag ccagttcggg ccacatacct ttaatactgt tcaggctgaa
     8581 attatatcgg tattcattca ttgcagtaat ctggaaaact ttcttcgcga tggaaggacg
     8641 caattccagc tttactcccg ggttgctacg tccattaata tcgaagaaat tactgattcc
     8701 accaatagct gcgacagtat caaaaagccc cagaaaacgg acttcacccg aaggtttacc
     8761 atcataaaaa tcgcctctga gccctttagc aatagctctg gctatagcag gatcctgttc
     8821 cataacccga ttagcaaagt gccttgccgc cgctgctccc cggctaaaac caaaaacatc
     8881 aaactggatt tttgcgatac aaaaatcagg atttaaattg aaccccataa attctgataa
     8941 cgcctgggtg attcgttcca ttgcctcgtc ggttttcgtc accacacctt caaatatatc
     9001 cagaatactg gtgcctaatc ccatgccaat gactgaatct gcttcaccag ccgcagtccc
     9061 tatcccggaa atgtaagtct taatttgagc tgacgtctgg tcttttttaa gtacctgatc
     9121 tggatgatag agtatattaa gccagtgaat attggaataa tatccacgat aactgccatt
     9181 cgagatattg ttcttgttaa tttcttcaag aaaatcctca cagctttgtg catctttttg
     9241 atttaccccc tgtctggctg cactacattc attaaatttc atcagacgag atgctgtatt
     9301 ctcccgattg tttccggtac catcgaaaaa tataccaatg gttaaagtca caccccgcgc
     9361 cggttttttc tttgcaacaa caactttcca gtcattatga atactggttt tatgggtggc
     9421 atctgttacg tcatatccct gatattttgt cattctgttt ctccatgaat ttttaccttc
     9481 ccaaactcaa cttttactac tgacggaatg gaggtgttga ctggcgctgt tttaccattt
     9541 tcatcggtga tcccctgtag aatatcacct tcagccgttg ttaatcgata aggcgtcgaa
     9601 ggttgcggca ctcccgcttc atcggtaaga ataaaaccgc caccaaaccc tcgcggcatt
     9661 tcaacagacg caatatcaag acttgttggt cccatcttct gcatattcgt gctttttaac
     9721 aggatctgtc ccgggcatcc cagctcaata tttccaccgc ttagtttgat ataagctcca
     9781 ccacatgcca gtatcagttc ctgattagca gaaacagtca ctttgccctc agaactttga
     9841 atatcaagat tctgttggga actcattgac aaatcgtcac tttgagcctg aatatttacc
     9901 cgcccttttg ctgctaacag ttgcatcccc tggtttctgc tcaacaggct aacagcacct
     9961 tctgctacaa ctgtaaatga ctgcccggca ctaatatcag tgttatctcc cgacattatg
    10021 cccacgcttt cactacccga agcaattcgt aacgcccgtg cactaaccat gccaatccca
    10081 tcaggagcat gcaacagcat tcccggcgca gtgagatctg ttagcgatga attcaaccgt
    10141 tgctgacaac ttatatctcc ctgagtagca tttgcagcag ttgcggcctt cgccatactt
    10201 cgggccagag ataatgcctg ctccagttgg cggatagctt cattcatatc cagcatcttt
    10261 ccttgtgcct gaggctgcat atctgcactg ataaatattc cttttcctcc ccggatactt
    10321 atccagtcat cagtcctcag ctcagcgcct tctcccctga gtttccggga ggcattaaca
    10381 ttatgaccaa gattaagttg tgtcttaccg ccaaactcgg tactgatctt gatatgctcc
    10441 tcaccgcgtt tgtcctccat ccgcaacttg ttatttgccg gcgtacggat aacattgcgc
    10501 gtaccgttct tatcggtaac gtgatcaaca tgacgtgaat catgcagggc atgcgcgata
    10561 taggggcgat ccggatcacc ttcctcaaag gcaatagcca cttccgttcc ctgaatcagc
    10621 ggaaaatgaa aaccataagc atcaccactg tagggtttgg ccagacgtac cggcatgctt
    10681 tcctgtcccg gatttttatc atcacggtca gcatcaaatt tcacccggta catgcctgat
    10741 gcatcctgcc aggcgtagat atccccttct tttgcactgg tcacacgggc cgtcatggta
    10801 ccggtgattt tcggacgagg aagtaacggt ggtcgccagc agataacttc actgtaagga
    10861 acgcccttca gtacaacctg cagggcatcc ttgcgacttg cagaaaaacc gacacaggta
    10921 agcaacaccg gattgcgcag cagcgcaggc aacgtcggcg gaagactgtc ggtgatagtc
    10981 agaacctgag caggcgccag cgtggcgtct gtgctgttgc cggttatccg tacctgctcc
    11041 gccagataac gttcatgacc aaggcgggca taaaaattgg ccgtctccgg caccgggtcg
    11101 attttatccc cccgttccag atgacggaaa tggtagtggt acacctcacc atagttaatt
    11161 ccgtcaccat caccatgcgt catgtctgcc ggggcggact gcaacagata ttgtgcttca
    11221 cgatggttat aagcctttgt ggtgacactg gcctctgcca cattgtgcgt cacattcagc
    11281 ccccacactg aatccgctct gttatcattc attccggacg gactgtttat cgccagcgtc
    11341 ttatcaaacg tcagcgcagc ctggctgtca ccaaaatgca ctatttccgt ctgcgtgtcc
    11401 ggttgcaggg tgaaaaaata aaaaatcccc acttcagcca gcaaacgctg aataaattgc
    11461 aggtcacttt cctgatactg gttaatttgt tcacgcttcg gatacgtttt tttgagacgg
    11521 aactcaaact cccagccttt caggccgtgc tcctggagaa cctctgtcac cacctccggc
    11581 accgatttgt taacgaaaaa tcggtgagaa cgatgctgcc tgtccagtag tttaataaat
    11641 ggttccagta tcacctggta ttttgcctgg tcggcggagc ccgatatacg acgaaaatct
    11701 gtaatgacac catgtactac tttctggctc acctgctcct taagccctcc cgtacccatt
    11761 gtaaatgtgg ctgatttacg cagcagcagc ccggcatcca ggtcgttgtc agtactggta
    11821 aaattgatga tgtaataata ggtctgactc atctcctccc tgccgctgaa atgctcaaca
    11881 tccagtggtg acgggcagga gggaataatt aaatggtaac gattcaggga agcagagata
    11941 gtttttgaaa gtgtatctgt cagactcata ttgttaactc catgtctgta ccggtattga
    12001 actgcaatta tataatgatg gccgttaagc tttattttca gagagaatta ctgataatca
    12061 tcccattcat cgtaaatata tgcatatgca aatcgataca tcagataacc gacaaaaaat
    12121 aacgcaacag agagccctcc ccacagatat ttaaattcag cagatgagag aaaaaagaac
    12181 cagactgtaa atacaatacc cgacgcacct aaccccatac ctgcgccacg gataaatcgg
    12241 gaaagcatta taagtaatgt tctttttatc ttcataaacc accgaaaaaa tattcgcata
    12301 tccggtcaaa ctcagccgaa tttcggtctg cgacaataca ggctcgttca aacgggcgca
    12361 caatatcttc aacaagaaag tacaaaagat ccaaatcccc ctgagctttc agcttatcat
    12421 gcaacaccgg atttctgtct ttcagaaaac gggatgtgta aatcgcacgc gatgtctctg
    12481 cgccaacaga taataacaac ccggcaaaac tccctgcaaa aaaagcagcc agccctctgg
    12541 ataccagaac ctctcccagt ttaaccccgg tcaattcagc aaatgccatt ctacctgcca
    12601 gatctcctga aacgttcttc acaatggagc ctgttttttc catattgatc cgggatacaa
    12661 agtcttcaag acaaatactg ataattttcc caataatatc acggctgaaa atgccttttt
    12721 ctaccagtct ggccattctg aatttgtcat tctggttttc ccgcctatgc ccggtatcta
    12781 taaaatcata tcccatataa ccgagactga ctggcaaaga tataagtccc ttcccgacgg
    12841 cccccagaaa cccatcccca ttaatattta cggcctccag catatcccgg gcaatttctt
    12901 tagcactgtc catatatgct ttcctgcgga taatcgctat tgattccatc aaacgctatc
    12961 acaataccat cctcatcatt ccacgtcaga gacaggtacg tcggatgctg tctggcagca
    13021 agatagctga gcaactgccg gctcagtacc ggcagcagtt gctggttcag cagactctcc
    13081 acattccggg cacccgtatc cggcagcagg caggctgatg tcagggcatc gaacacacgt
    13141 tcatcaaccg atgtgctcag tccataatgg cagtgcaggc gttcacagac ctgtgcaagc
    13201 ttcatctcca caatgatgcg catggcagac tgtgtcagcg gacggtaaat cacggtctga
    13261 aagcgagcca gcagggcagg ctggaagtgg tcgcgcagca gtggacggat aagctcatgc
    13321 agctccgatt ccgttgtctc cggcttttcg ctcagttgct gcatcagaag gtcactgccc
    13381 aggttggagg tcatcagtat gaccgtatta cggaagtcaa tttcacgccc ctcaccgtcg
    13441 cgcataaagc cccggtcaaa cacctggtag aacagattca gcacatcccg gtgggccttt
    13501 tccacttcat ccagcagcac cacactgtac ggacgcttac gtacagcttc cgtcagaatc
    13561 cccccctgac cgtatcccac gtacccgggc ggtgaaccct taagctggga aaccgtatgg
    13621 ggctcctggt attccgacag attgatggta atcagtgatt tttcgccgcc gtacatcacg
    13681 tctgccagcg ccagtgccgt ttcggttttt cccacaccac tgggaccgac cagcaggaac
    13741 accccctggg ggccgttacc ggatgtcaga cccgttttcg ccgcacgcag acgctgcgca
    13801 atggattcca gtgccacgtc ctgtccgacc acccgtctgc cgatatcctt ttccagatgc
    13861 agcagttcca tctgttcgtc cttcatcagg gaagataacg gcaccccggt ccagtccgcg
    13921 accacaccgg caacggtgcg tacgtcaaca tccacactga gcaggggctg attctgctga
    13981 atgtcgtgca gtgcctgctg cagatgcagt gtctcactct gacgggaaat atcgctgcga
    14041 ctttcaagta actcaccggt gagttcgcac tcccgggcgt actgctgctc caggtcatca
    14101 cgtgctgtca tcagctcgtt ctcttcctgc tcaatggcag acagacgttc gcc
//
LOCUS       DAZGSP010000127        14110 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZGSP010000127 DAZGSP010000000
VERSION     DAZGSP010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.128"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(578..1039)
                     /locus_tag="ACHK74_004344"
                     /pseudo
     CDS             complement(578..1039)
                     /locus_tag="ACHK74_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1347..1460
                     /locus_tag="ACHK74_004345"
     CDS             1347..1460
                     /locus_tag="ACHK74_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP0223055.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGN"
     gene            complement(1704..2408)
                     /locus_tag="ACHK74_004346"
     CDS             complement(1704..2408)
                     /locus_tag="ACHK74_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HFP0223056.1"
                     /translation="MRRLPLMVGVIVGVMLAVGCHDRRAGVKTLLGDGMDQWGQKLPY
                     DHWQFNFFYPKNLPALVTMVYLEDGDVRESIFRQLDAARPSQSSVDTWSQRVGSFSAS
                     FNIGKALPVRMTVCWDSVIDKKAYETEIWFSRDTWQQMLTAYPDTYKPGKTYYRDNMI
                     IGLAPGGAVRIWLEDNGEPVVLQHPARQFTLTGDDMLICKNVPNQIDFSYIKANGYDP
                     FIRDFIKEKPYPYGHW"
     gene            complement(2419..4110)
                     /locus_tag="ACHK74_004347"
     CDS             complement(2419..4110)
                     /locus_tag="ACHK74_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HFP0223057.1"
                     /translation="MVEEDEKNVPGDTCYYRYEIIGDSTHRTSIHNSFKTIQCRRVPV
                     RGITLTVGVFFDGTGNNRANADDLRLAYAHCANLVDEERARACEKYEKRARAGLGNAS
                     WQGGHTNISRLFDLYQQSDELNDSETEAQVKAYISGIGTADGESDSILGKALGSSLIR
                     QFEGVVTKTDEALEKISAELTRFIKLNRDKVAIAKVQFDVFGFSRGAAAARHFANRVM
                     NQDAAIAAAIDKGTDLTGHHGKLAGEVRFLGLFDTVAAIGSLLNFYDVNGRSNPGVNL
                     ELRPSVAQKVFQISAMHECRYNFSLNSIAGMWPELALPGAHSDIGGGYNAGEDEISLL
                     TMPDFEVTRDSADEMQTEVYRKAEKMRQVLYSLPALKYLMPHGEVETKMISWPLVNQN
                     KARASIFEKKVGAAVFMTRRDVPNDWEKVSLRVMLDAAQDAGAVFTPILSSNKELALP
                     SELLPLCDKAIAQGRAVRRGAEPEGFTAEDMRTIGRYLHCSANWNIDSDLSLWVSPSS
                     GEVFLRNNYAPTDERSFVWPMAPGKGWVRTVWRMDDQQQWEERARVNADALDGLF"
     gene            complement(6298..6492)
                     /locus_tag="ACHK74_004348"
     CDS             complement(6298..6492)
                     /locus_tag="ACHK74_004348"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFP0223058.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAK
                     GENIGWCIARAEYCKKTGRF"
     gene            complement(6750..7218)
                     /locus_tag="ACHK74_004349"
                     /pseudo
     CDS             complement(6750..7218)
                     /locus_tag="ACHK74_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562850.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7378..7632
                     /locus_tag="ACHK74_004350"
     CDS             7378..7632
                     /locus_tag="ACHK74_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HFP0223059.1"
                     /translation="MKITTLSSRELNQDVTRAKRATNYGPVFITDRGKTAHVLLSIEE
                     YRKLTQQRRTLIDILAMPDADAVEFEPPRITVSLKPAEFL"
     gene            7632..8048
                     /locus_tag="ACHK74_004351"
     CDS             7632..8048
                     /locus_tag="ACHK74_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFP0223060.1"
                     /translation="MYILDTNVISELRKARTGRIDNNVAKWALHTDAADFFISVISIM
                     ELEQGILQVERRDMRQGIMLRSWMDQHVLPAFSDKILPVDTAVALRCSRLHVPDRRSE
                     RDALIAATALVHGMTVVTRNVGDFELTGVSIINPWA"
     gene            complement(8358..8561)
                     /locus_tag="ACHK74_004352"
     CDS             complement(8358..8561)
                     /locus_tag="ACHK74_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223061.1"
                     /translation="MPELPHFAPAPVPCPASALASQGGVELLTFGRLYRLTPGFGGFT
                     PLPYTAWSAAFHVAGAAYHGDDI"
     gene            8946..9308
                     /locus_tag="ACHK74_004353"
                     /pseudo
     CDS             8946..9308
                     /locus_tag="ACHK74_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            9312..9667
                     /locus_tag="ACHK74_004354"
                     /pseudo
     CDS             9312..9667
                     /locus_tag="ACHK74_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
     gene            9949..11088
                     /locus_tag="ACHK74_004355"
     CDS             9949..11088
                     /locus_tag="ACHK74_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HFP0223062.1"
                     /translation="MNKIHANYYLQDSTARQIVQRTMGIIPYSVNVMNEHGMIIASGE
                     ASRLHQRHEGAVLALTENRVVEIDSATANKLKGVKPGINLPINFRDQLIGVLGITGEP
                     GEVKAYAQLVKMASELIIEQMALLEQKQWDKRYREELINQLILRDPPPDSLNAVVSWL
                     GINLQLPRVVVIMELQQPDRDALHNLMEYFEYRARDHLVTFSDFNDLIILKPISLKNG
                     VWDKSLELSDLQRLKHYAQSCGFSRLIVGGYFSGENGLHRSFQTAKSTQSMAKRLKLK
                     HQFIFHDDYALASLLDGLSGTWQAEELSKIWRNLVAKDTKGMLQKTLQNYFDHNCDLS
                     QTATGLHIHVNTLRYRLQRVEDITGIQINNLKQLFWLYIGMELQR"
     gene            11208..12476
                     /locus_tag="ACHK74_004356"
     CDS             11208..12476
                     /locus_tag="ACHK74_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004090684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFP0223063.1"
                     /translation="MVSITATGAIIALVVAIVLILRKVSPAYGMIVGALVGGLVGGAD
                     LVQTVTLMVTGAQGITNAVLRILAAGVLAGVLIESGAANTIAETVVKKVGESRALLAL
                     AIATMILTAVGVFIDVAVITVAPIALSIARRAGLSKLAILLAMIGGGKAGNVMSPNPN
                     AIAVSDTFHVPLTSVMMTGIIPGLFGLIVAYFLAKCLSQKGLMVMPDEMISHDNHGKQ
                     PGFLAAISAPLVAIVLLALRPVAGIVIDPLIALPVGGLAGAILMGRFKQSNAFMMSGL
                     TRMAPVAIMLLGTGTLAGIIANSELKDVLIHGLTSSGLPSYLLAPVSGAIMSMATAST
                     TAGTAVAAGVFSQTLLGLGVSALAGSAMIHAGATVLDHLPHGSFFHATGGSVGMLIHE
                     RLKLLPYETLVGFVVALISTLMFGVFGLAG"
     gene            12484..13629
                     /locus_tag="ACHK74_004357"
     CDS             12484..13629
                     /locus_tag="ACHK74_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP0223064.1"
                     /translation="MKIVIAPDSFKESLSAMQVATAIEQGFREIYPEAEYVKLPMADG
                     GEGTVESMVEATEGHYLFQNVTGPLGTPVQARWGMLGDGKTAIVEMASASGLHHVPPE
                     CRNPMLTTSYGTGELILAALDHGVERIILGIGGSATNDGGAGMLQALGVAMLDTNGKS
                     LPFGGGALSNLALIDASGCDARLKNVSVTVACDVNNPLCGATGASAIFGPQKGATPEM
                     VASLEAALTNFGIRLQDATGRNVMTTPGAGAAGGMGAALLGILNAELKPGVEIVIDAL
                     HLDSLVADASLVITGEGRLDSQSISGKTPIGVARCAKRYQKPVIALAGGLTTDHQIVH
                     EHGLDAVFSVLTQIATLPEALQDAEYNLRVSARNIASVWKLSQQQMR"
BASE COUNT         3456 a         3680 c         3369 g         3605 t
ORIGIN      
        1 ctgacgcacc ggcgaaggca ataaaaatga gatacatttc acttcgcagc aaaaaacgaa
       61 attctacaat ttttctgatt ttactgacag aaaagcctga gagttaaatt taaataactt
      121 aaaagattat gtggtataat caggttcata caggcctgat tattttacgg ggatgttaac
      181 tctttcaaat caacagtcta cggagaagca atgtggttat ggtcaataaa tgttcactga
      241 tttctttcac ggtaataccc ggggcacttc ttgtcctctg gtaacaaagg taactgtaca
      301 ttcgtctttg caactgacag tgcatcacgg ttttgaagta cctgctgctg ttgttttttt
      361 actcagaaag cgcagttact ctttatattt tcccagccta tctcttattt taatatacat
      421 ccactgtctg gcgttttaag gggctggctc actggccaag ttgacattaa tcatcattcc
      481 tgtctttgtc agcggagact tctgctgtca gttttttcat ccatacagag tgacattaat
      541 tatccgtaag cgcaccgtga aggacgtggg gtaaaaatta gtttacagat agagtgacgt
      601 tccagggcaa caactctttc acgcggttgg ctggccaggt gttgatgaca ctgatcacgt
      661 ggcgtagcca cgtttccggc tcgattccgt taagtttgca gctaccgatc aggctgtaca
      721 tcactgccgc actctcgcct cctccatcag agccggagaa catgtagtta cgccgcccca
      781 gtgcaactac ccggagggcg ttttcacaca gattattgtc gatctccacc cagccattgc
      841 ggcagtattc gttcagggcg tcccattgct tcagcagata ggtgaacgct ttcgccgtat
      901 ccgagtgacg cgacagcgtg ctcatctgcc cctggagcca ctcatacaac gactgcatta
      961 gcggtaccgt tctggctttt ctgaccgcca gtcgctcttc tgccggactg ccgcggatct
     1021 cagactcgat agcgtacagt gcctcgatag ggctgcgttt gggaaacgta caatattgta
     1081 cggttgcgct attgcggtag ggtgtactcc aacgctatac ggtatttggc agacctttag
     1141 ggacatgaga taacctaaat gcaataagag aaaagcaata acttaaaggc gatagctgat
     1201 cacgatgaat caatcagtta cttatccgca tcgattaaaa aatgctccta acgtacaata
     1261 attccgtttc ccaaaccgac ccctctcata gctcacgctg ttggtatctc agttcggtgt
     1321 aggtcgttcg ctccaagctg ggctgtatgc acgaaccccc cgttcagccc gaccgctgcg
     1381 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta acgccactgg
     1441 cagcagccat tggtaactaa acaaggtgga ttccgatatt agagtcttga agtgatcacc
     1501 caactacggc tacactgaaa gaacagtatt cggtatctgc gctccaccaa gccagttacc
     1561 acagtcgatt agaagaggtc gaagacttct aatcggacgg aaaaagccgc cgcaaggcgg
     1621 ctttttccgt ttacaagccg aagattacgc gcagcttaaa aggatctcaa gaagatcatt
     1681 ttaattttgc tgatcgttgc tccctaccag tgaccatacg gatagggctt ttccttgata
     1741 aaatcgcgga taaatggatc gtaaccatta gccttgatgt aactgaaatc aatctggttg
     1801 ggaacgtttt tgcatatcag catatcatct cccgtcagag tgaactgccg ggcaggatgt
     1861 tggagtacaa caggctcgcc attatcctcc agccagatcc gcacggcccc tcccggtgcc
     1921 aatccgataa tcatattgtc ccgataatac gtttttcccg gcttataggt atcaggatag
     1981 gcagtcaaca tctgctgcca cgtatcccgg ctgaaccaga tctccgtctc atatgccttt
     2041 ttatcgataa ccgaatccca acacaccgtc atcctgaccg gcagtgcctt gccaatgtta
     2101 aaactggctg aaaaactgcc tacgcgctgg ctccatgtat ccacactact ctgacttggt
     2161 ctggctgcat caagctgccg aaatatgctt tcccgtacat caccgtcctc cagatacacc
     2221 attgtcacca gtgctggcag gtttttcggg taaaaaaaat taaactgcca gtggtcataa
     2281 ggcagcttct gcccccattg atccataccg tcgcccaaca gcgtcttcac gccagcccta
     2341 cggtcatggc agccgacagc cagcattacg cccactatca cgcccaccat cagcggtaat
     2401 cgtctcattc ctgcctcctc agaacagtcc gtccagcgca tccgcgttta cccgcgcccg
     2461 ctcttcccac tgctgctggt cgtccatccg ccacaccgtc cgcacccagc ctttgcccgg
     2521 tgccatcggc cacacgaacg agcgttcgtc agtcggagca tagttatttc gcagaaaaac
     2581 ttctcccgag gacggactca cccacaggct caagtctgag tcgatgttcc agttcgccga
     2641 gcaatgcagg taacgcccga tggtccgcat atcctccgcc gtgaagccct caggctccgc
     2701 gccacgccgg acagcacgcc cctgagctat ggctttgtca cacaggggaa gcagttctga
     2761 cggcaacgcc agctctttgt tactgctgag tatcggagta aacacagccc cggcgtcttg
     2821 tgccgcatcc agcatcaccc gcaaactcac cttctcccag tcgttcggca catccctgcg
     2881 cgtcataaac actgccgcgc ccactttctt ctcaaaaata gacgcacgcg ccttgttctg
     2941 attgaccagt ggccacgaaa tcattttcgt ctccacctca ccgtgcggca tcaggtactt
     3001 cagcgccggc agtgagtaca gcacctgacg catcttctcc gccttacggt acacctccgt
     3061 ctgcatttca tccgcgctgt cgcgcgtcac ctcaaaatcc ggcatcgtca gcagggagat
     3121 ttcatcctct cctgcgttat agcccccgcc aatgtccgag tgcgcccccg gcagcgccag
     3181 ctccggccac atccccgcga tgctgttcag gctgaagtta taccgacact catgcatcgc
     3241 gctaatctga aacaccttct gcgccactga aggacgcagt tcgagattca ccccgggatt
     3301 gctgcgtccg ttaacgtcgt agaagttcaa caggctcccg atagccgcca ctgtgtcaaa
     3361 cagccctaaa aagcgcacct ctcccgccag tttcccgtgg tgcccggtca ggtctgtccc
     3421 tttgtctatt gccgccgcta tcgccgcatc ctggttcatc acccggttgg caaaatgccg
     3481 tgcagccgcc gcgccgcggc tgaatccgaa cacgtcaaac tgcaccttcg caatcgccac
     3541 tttgtcacga ttaagtttga taaagcgggt cagttctgcg ctgatcttct ccagtgcttc
     3601 atcggtcttt gtcaccaccc cttcaaactg tcggataagg ctgctgccca gcgctttacc
     3661 cagtatcgaa tcactttctc cgtctgccgt cccgatgccg ctgatatagg ctttgacctg
     3721 cgcctcagtc tcgctgtcgt taagttcatc gctttgctga taaagatcga acagccggga
     3781 aatattagtg tggccgccct gccagcttgc attacccagc ccagctctgg cgcgcttttc
     3841 atacttctca catgccctag cccgctcctc gtcaaccaga ttggcacagt gggcatatgc
     3901 cagccgtaga tcgtctgcgt tcgcccggtt attccccgtc ccgtcaaaga acacccccac
     3961 cgtcagcgtt atccccctga ccggcacccg ccgacactgg atggttttaa aactgttgtg
     4021 tatgctggtt cgatgggtac tgtccccaat aatttcatac cggtaataac aggtatcgcc
     4081 cggcacattt ttttcatctt cttcgaccat aaaaatcctt atttatctga aagataaaat
     4141 cagtagcaaa atccttcggg acttttttac atctgcctgt tagacgtatt taccataatc
     4201 ccttaattgt acgcaccgca gaaacgcgct cagcgcgatt ctggcgcaaa aaccgcaaaa
     4261 atagatgttt tcccccgggt aaaccggaaa aatgcgtcag gattgttttc atggcactgc
     4321 atgactatgc atgaaactga atggcgatcg gtttgggcgc gcctgatgcc cataaggcgc
     4381 attttcggac gttttcagcc ctgatgagaa gaaatcagac agtagttaca gacgagtcgt
     4441 gagcgattca ccccgggagt catcagcgag tcatccagta tttttcctcg cgactctctg
     4501 gcgactcgct ttttctgaac accagagaga cagtgtgttg agtcatcgat aaatcaccga
     4561 cgactcgttg ccgaatcatc cagtagtcgc cgacaactcg cttttgtata aatctgaata
     4621 aaaaaatata tttttcaaac tataacaaca tgatttaaaa agcaaatcag aaaaaagttg
     4681 gttttgcgtg gggggtgggc atccggggca tgagaacaga ctcgcgtttt tctggagaaa
     4741 ctgcggggat ttttgattaa acaatactca ccgcagagcg gaattttatg caacgccggc
     4801 tgtgcggcac agggattttt aatccccagc atgtcagagg gtgcggatga cggttcccgt
     4861 ctggcgtttt catgggcgcc ggttgtcaaa aaataccagc gtgatcggtc gaaggataag
     4921 cgcagattaa tcagtgatga ttgagcgcga atctgacaac aagtatagcg acacagcaac
     4981 aaaaaagacg ccagaaacgc gctcagaggc tttgtaacgc gattttttac atgaggcaat
     5041 ggtttcgctt tgaggcgttc agccgtgttc agggcgcgat tctggtacat acctgactgt
     5101 aatcatccct acgaactact ctttgcacca gaaacgcgca ggagtgtttt agcgtgtctc
     5161 cggcgtgatg tcgagttgtg cggtaatgag tgagcgaaaa aaatcccctg gattacaggg
     5221 ggaacccatg atcagacttt gaagtttatt tgttatttga tggaatgagc acgaatcccg
     5281 gagatgacgt tcaggtggca ggaaacgcgc tcagaagctt tctaacgcac ttttttgcat
     5341 ctggcaatgg gtttcatatg ttttggtggg ttcaggctgt cagagacgtt ttgagcgcgt
     5401 ttctggcgct tgctgctact gccaaaccat ccccatggcc agttgatgcc agaaacgcgc
     5461 aggaattttg tagcgcgtct ccggtccctg atcaatgaat tccctgtcat taacatgcgt
     5521 ttacccggaa acgcgccctg atgttctatc gtatcgcggg aacggtgtcg agggtgtcgg
     5581 ggcaacgccc tgaccaggtg gtaatcgtat agccgtgcgt gcgcggttat acgattacac
     5641 atcctgtccc ggaatcagga ttgtttttcg cgcacaactg cggcaaattc atcccgttgc
     5701 ggttgcaacg atgggtatat caaaactacg aaaattattc aggcatttgc tacccccctt
     5761 gccctggcct ccaccagtca cgggggattt tttcgcctgt cctgattgtc ttttgccgat
     5821 ggttacggca gattggaaaa gagcgcattt gtggtgctct ctccaaaata atatttctca
     5881 ttactcaaac tctgatggct tccccctgta atccaggggg attttttaat ttatcgctat
     5941 gcttcgtaat tcattcagaa tacagacagt atggttaatc agtttttcat cattttttcg
     6001 gtgtttgccc tgtcctgcat cagaacgcta aaaaaacagg attggaatct ctttatgacg
     6061 aatcctccat ttcaggattt tttatgtaac gtctactaca tgcgatccgt tggtgaaccg
     6121 ttagttatat attggttata tgttagttat atcttagtta tacttttgct gcgcgtcagt
     6181 tacagaggcg ttttgagggt aaattctgga ggcacgtgac cgactgtata caaaatgctg
     6241 gcgcattttg atacgtcgac gtgccaaagg ggacgcccct ttgaaacccc gccagaatta
     6301 aaagcggcct gtctttttac agtattcagc tctggctata caccaaccta tattttctcc
     6361 tttggcataa tgaagcgcat cattccattt tttccatgct ccaatgtaat caccttcacg
     6421 ctccagtctg acagcttcgt ttgcgggtga aaaatattta ttttctaaat aaattgcttt
     6481 gtcctgattc atattcgtaa tttatatttt tatttgaatt aaaaaagagc gagcccatcg
     6541 ggttaataca tattggcatg actgttgata tatcagtcca acagcacgct cgtaatcgtt
     6601 ttaacgtgat tttcgcaaca tggcaatccg ggttcatctg tccggtaatc aataaaaacc
     6661 gtgcgaaaaa cgggacagga tgtggccatt aatgctaatg tatgcataca ttagcattaa
     6721 tggccctgac aaataccagg catccggtct caggatgcct tgattcttgc ctggtagatg
     6781 cgctttaccc gttcaatttc tgccagcgtt gcggaccagt gtagttcggc ggcatgacga
     6841 tatctggcag ccattttttc caccatctcc ggattgctgt ccgcgctgcg gtgctgacgc
     6901 cccttctccc caaaaacttc aacaaccagc cactggcttc ctgttctgcc gaacaatgcc
     6961 ttgcacaaac gtctcacagt cagcggggtt aaatctggat cgggatcgta ggcagtggta
     7021 gcaaaaataa agcgcagcat ttctgatgtc ttgtaacgct ttacggcagc tgccagacgt
     7081 gcggcgcgtt ggggtgcttc ccagcggtcg tcactgtaat tagcaacttc agccagataa
     7141 tccttttcaa ggttttcagc cgtgtaacac ctgccgccta aaaaaaaacc gcatggcctc
     7201 cctggttttg tctctcacat atcatccctc attaatgcta atgtatgcat acattagcat
     7261 taatatctga cagcaacaag cggcgaagac tacccctaat caagtacatt tcttgcattg
     7321 tactatttca cgccctacaa tgtagtaacg tacctatata catcaaaagg caacagcatg
     7381 aagattacaa ccctatccag tcgggaactt aatcaagatg taactcgagc caaacgcgcc
     7441 acgaattatg gtccagtatt tattactgat cggggtaaaa ccgcacatgt acttcttagt
     7501 atcgaagaat accgaaaatt gacgcaacaa cgacgaactc tcattgacat tctggcgatg
     7561 cccgatgctg atgcagttga atttgaaccg cctcgtatta cggttagttt aaaacctgcg
     7621 gagtttttat agtgtatatt cttgatacta atgttatctc tgagctacgg aaggctcgga
     7681 caggacgtat agataataat gttgcaaaat gggcattgca tacagatgcg gcagatttct
     7741 ttatttctgt tatttccata atggaacttg aacagggaat attacaagtt gaacgtcgag
     7801 atatgcgaca agggattatg ctacgttctt ggatggatca gcatgtgtta ccagcgtttt
     7861 ctgataagat acttcctgta gatactgcgg ttgcattacg ttgttctcgt ttacatgttc
     7921 ccgacaggcg cagtgaacga gacgcattga tcgcagcaac ggcgctggta catgggatga
     7981 cggttgtaac cagaaatgtt ggtgattttg aattaaccgg agtatcaatt attaatccct
     8041 gggcttgata tcgcttccct tcttctaggg gtccgcttgg aaaacggaaa ttttcccacg
     8101 ctaagagaat tgagttgaac gagtggttac atccaagcca gctaactcac tgaacgtaaa
     8161 ggctgtacga aatgttccga ccgtccggat tcctgacagt catttggcta aaaaatagtc
     8221 ttaacgtggt ttattttccg aattccaggc tgaccccata aaagcatcct tcagttcagc
     8281 gtcgattctt aaactcatat taacctgtgc cattcatcac ctcgtcatta ccacgtatat
     8341 acacagtata taattattca gatatcgtca ccatgatatg ccgcgccagc aacatggaag
     8401 gcggcactcc atgctgtata tggcagagga gtaaaaccgc cgaatcccgg cgtaagccgg
     8461 tacagtcgtc cgaatgtcag tagttctact cccccctgcg aggccagtgc tgacgcggga
     8521 catggaacgg gtgctggagc gaagtgggga agctccggta tactggccaa acctacacgc
     8581 ctccttccgt ctccgggaag atgctggctt cgtaccctgc gtgcgtctga tctgtagctg
     8641 gccgtggcac ggctacggac agattctgct ccgcaacagt atcggcgtgc ctgacctcct
     8701 gaaagtcccg taacgcattt tctcacctcc catgccctga cgctattgag cgcgttacag
     8761 aacaatttaa gtcatttaac gagcctccaa cggctcgatc gaaataaatc aaatcgtaat
     8821 cgtcaacgat atatgagagg agaccccgct cgccgcaaat cgatgaccga acgtagtgag
     8881 cgaatcgacg aggaagcgga agagaacctg tacgaaaatg gagcaattac gccccaccgt
     8941 caagaaatga gctggctctc cacggcgcgc catcatagac agatgtggct ctgcaactgc
     9001 gccatgcgat cggagaccga ccagtaatta tctttaatgc ccggttcgac acccgcattc
     9061 tgaaacagac tgctgccgca catagcgatc cggctgactg gctggaagaa ctgacagtat
     9121 attgtgcgat ggaactggca gcaggatatt atggggccac caaccgctat ggcacgattt
     9181 ctctggcctg tgctgccagc aggccggact gacatgggaa gggctggcac actcagctga
     9241 tcgccgacac acggatgacg tcaggggtgg taaacgctat tactgcatat catcaggaat
     9301 tgctgcagct tatgtactgc gataaccctg aactcaacta tgcaaacctg aaatccctgc
     9361 aaaaacagct ggatgaaatg tatacctgca tccgtaataa tcagatgatg ctcccaaact
     9421 acggaaaaat gcgccggtat ggtgaaccgg tttcaacaac gtttgtagag tcgacaatca
     9481 acgaagtgat cgccagacga tgacccaaaa gcaacagatg cagtggggta gggaaggtac
     9541 gcattatttg ttacagacca gaaccgctgt cctgaataat taactgcagg acaaattcgc
     9601 cagttggtac ccgggttgca cgatagacgc tcagagtgga ggaaagttat ccgccatggc
     9661 agcataacta cttccccagt tttttcagct ctcccttacc aatatcctac gacgatcaca
     9721 tcgaaaaaaa aacacggaac aggcgaacag aaatataatt aattatttga gtttaaagga
     9781 aaaaagagga tacaagcttt ttgttgtatt tttatttgta taaaccgaca aaaaagaggc
     9841 catacttccc gctttctttg tctgtttaca cggaacattt caccactgga tacgctaata
     9901 gatcggatat gagcacatat tgcgcccgcc tttcatgcag agcataaaat gaacaagatt
     9961 cacgctaatt attacctgca agattctacc gcacgccaga tagttcaacg cacaatgggg
    10021 attattccgt actcggtaaa tgtgatgaac gagcatggga tgatcatcgc ttccggggaa
    10081 gcctcacgtt tacatcaacg ccatgaaggt gcggtactcg cattgaccga aaaccgggtc
    10141 gtcgaaattg attccgcgac cgccaataag cttaaaggcg ttaagcccgg aatcaattta
    10201 ccgattaatt ttcgcgacca gctgattggt gtactgggca ttaccggcga gcctggtgaa
    10261 gtgaaggcat atgcacagtt ggtgaagatg gcttcggaat taatcattga acaaatggct
    10321 ctcctcgagc aaaaacagtg ggataagcgt taccgcgaag agctgattaa tcagctgatt
    10381 ttacgcgatc cgccaccgga ctcactgaat gcggtggtct catggttggg aattaatctc
    10441 cagcttccgc gggtggtggt gattatggaa ctgcaacaac ctgatcgcga cgcactgcat
    10501 aatctgatgg agtattttga atatcgcgcc cgcgaccatt tggtcacgtt ttccgacttt
    10561 aatgatctga taatcttaaa acctatctcg ctaaaaaacg gcgtctggga taaatcgttg
    10621 gaattaagcg atctgcagag acttaaacat tacgcccagt cctgtggttt ttcccgactg
    10681 attgtcggcg gctatttctc tggagaaaac ggcctgcatc gctcttttca gaccgcgaaa
    10741 tccacccagt ccatggcgaa aagactgaag ttgaagcatc agttcatttt tcatgacgac
    10801 tatgcgctgg catcattatt agatgggctt tctggtacct ggcaagcgga agaattatcc
    10861 aaaatctggc gcaatctggt tgccaaggac accaaaggta tgctgcaaaa aacgctgcag
    10921 aattattttg atcataattg tgatctttcg caaacagcga ctggcttgca tattcatgtc
    10981 aatacattac gctatcgact gcagagagtg gaagatatta caggaataca aatcaataac
    11041 ttaaagcagt tattctggct gtatatcggc atggagttac aacgctgaac tgtatttccc
    11101 tccaaatttt tcgccgtctg acgtcgtatt tttagccgat acacacaagt ctaacgcggc
    11161 tgctttctcc atactgcccc ctgttatttt catggagaaa gcagaagatg gtttcaataa
    11221 cagcaactgg cgccatcatc gcactcgtgg tggcgatcgt tttaatttta cgcaaagtct
    11281 cgccggctta cggcatgatt gtcggtgcgc tggtaggcgg tctggtcggc ggcgccgatc
    11341 tggtgcaaac cgtgacgcta atggtcactg gcgcgcaagg cattaccaat gccgtgctgc
    11401 gcattctcgc agcaggtgta ctggccggcg tgttgataga atccggcgcg gcaaacacca
    11461 tcgctgaaac tgtggtgaaa aaagtggggg aaagccgggc gcttttagcc ttagctattg
    11521 cgacgatgat tctgaccgcc gtcggagtat ttatcgacgt ggcggttatc actgttgcgc
    11581 cgattgccct gtctattgcc cgtcgtgccg gtttgtcgaa actcgccatt ctgctggcga
    11641 tgattggcgg tggtaaagcg ggtaatgtta tgtcgcctaa ccccaatgct atcgccgttt
    11701 cggacacctt tcatgttccc ctcacctccg tgatgatgac cgggataatc cccggtctgt
    11761 tcggcctgat cgtggcttat ttcctggcaa aatgtctcag ccagaaaggc ctgatggtga
    11821 tgcccgacga aatgatttca cacgacaacc acggcaagca gccgggattt cttgccgcta
    11881 tcagcgcgcc gctcgtcgcc attgtgctgc tggcgctacg acctgttgcg ggtatcgtca
    11941 tcgatccgct gattgccctg cccgtcggtg ggttagccgg tgcgatcctt atgggcaggt
    12001 ttaagcaaag taacgccttt atgatgtccg ggcttacgcg catggcgccg gtagccatca
    12061 tgctgctcgg taccggtacg ctcgccggga ttatcgccaa ctcggaactg aaagatgtgc
    12121 tcattcatgg actgacctct tccggtctgc cttcttatct tctggcccct gtttccggcg
    12181 cgatcatgtc catggcaaca gcctccacca cggcgggaac cgctgttgcc gccggggtat
    12241 tcagccagac gctgctgggg cttggcgtat cagcccttgc cggttccgcc atgattcatg
    12301 ctggcgcaac cgtacttgat cacctgcctc acggcagctt cttccacgcc acgggcggca
    12361 gtgtcggcat gctgatccat gaacggttaa aactgctgcc ttatgaaacc cttgtcggtt
    12421 ttgtggtcgc cctgatttcc acactgatgt ttggcgtctt cggacttgcc gggtaaagac
    12481 acgatgaaaa tagtcattgc accagattca tttaaagaaa gtttatcggc catgcaggtg
    12541 gccaccgcta ttgagcaggg atttcgtgaa atttatcccg aggccgagta tgtcaaattg
    12601 ccgatggctg acggcggtga gggtaccgtc gaatcaatgg ttgaagcaac cgaaggccat
    12661 tatctgttcc agaatgtcac cggaccgctg gggacgccag tacaggcgcg ctggggtatg
    12721 ctgggagatg ggaaaacggc gatcgttgaa atggcctccg catccggact acatcatgtt
    12781 cctccagaat gccgtaaccc tatgcttacc accagttatg gcacaggcga actgatcctt
    12841 gccgcactgg accacggcgt tgagcgaatt attctcggga ttggcggtag cgccaccaat
    12901 gacggcggcg caggtatgct acaggcactt ggtgtggcga tgcttgatac aaacgggaaa
    12961 tcgcttcctt tcggcggcgg cgcgctaagc aacctggcgc tcatcgatgc ctcaggctgc
    13021 gacgctcgac tgaaaaacgt ttctgtcacc gtcgcctgcg acgttaacaa tccactctgt
    13081 ggcgcaacag gagcgtcagc catcttcggc ccgcaaaaag gagcaacacc agaaatggtt
    13141 gcctccttag aagctgcgct gaccaacttc ggtatacgac ttcaggacgc aaccggacga
    13201 aacgtgatga cgacgcctgg cgcaggtgcc gctggcggca tgggcgcggc gctgttaggg
    13261 attctgaatg cggagttaaa gccgggagtg gagattgtta ttgacgcgct gcatctcgat
    13321 agcctggtcg cggatgccag cctggtaatc accggtgaag gtcgtcttga tagccagagc
    13381 atcagtggta aaacaccgat tggtgtcgca cgctgcgcta agcggtatca gaaaccggtg
    13441 atcgcactgg cgggcggctt aacgaccgac caccagatag tgcatgagca cgggctggac
    13501 gcggtgttct ccgtgctgac gcaaatcgcc actctgccgg aagcgctaca ggatgcggaa
    13561 tacaacctcc gcgtttcggc acgcaatatt gccagcgtct ggaaattatc acagcagcag
    13621 atgcgctaat cttcagtcag gccggcaagc agtagctggc ctggatattc tttctcacca
    13681 ggcccatgct aaaacagcct gcttcagtca tcccacgcaa ggcactagca tggcaaaaaa
    13741 agaggggagt agtggatccc aaaagacggt taagcggtta aattgaatgg tcaataaggg
    13801 ggctgtttgg gaaacgaatc tatggtcact cccgtttttg caacaccgat tttgacgata
    13861 agtgggcttg cttgaatcta tccggcgtct gaatgggatt ttattcccgc gcctcgatga
    13921 gttccgcgcc tgatgaacct caagaaaata tacggcttca atgagccttt ccgtttcaca
    13981 ggttcctcaa caggccggtg ggccgttcat atcatcaata ccgtgatgct gatactccgt
    14041 acccaccgga accgcacctg atgcggttcc ggcttaaggg ctgtcaggac tcgcttttgg
    14101 ctgccatcaa
//
LOCUS       DAZGSP010000128        13917 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZGSP010000128 DAZGSP010000000
VERSION     DAZGSP010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.129"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            207..2651
                     /gene="dmsA"
                     /locus_tag="ACHK74_004358"
     CDS             207..2651
                     /gene="dmsA"
                     /locus_tag="ACHK74_004358"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFP0223065.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            2662..3279
                     /gene="dmsB"
                     /locus_tag="ACHK74_004359"
     CDS             2662..3279
                     /gene="dmsB"
                     /locus_tag="ACHK74_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFP0223066.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            3281..4144
                     /gene="dmsC"
                     /locus_tag="ACHK74_004360"
     CDS             3281..4144
                     /gene="dmsC"
                     /locus_tag="ACHK74_004360"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFP0223067.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(4180..4806)
                     /gene="ycaC"
                     /locus_tag="ACHK74_004361"
     CDS             complement(4180..4806)
                     /gene="ycaC"
                     /locus_tag="ACHK74_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFP0223068.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDTPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            5120..6268
                     /gene="ycaD"
                     /locus_tag="ACHK74_004362"
     CDS             5120..6268
                     /gene="ycaD"
                     /locus_tag="ACHK74_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0223069.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            6478..7908
                     /gene="ycaM"
                     /locus_tag="ACHK74_004363"
     CDS             6478..7908
                     /gene="ycaM"
                     /locus_tag="ACHK74_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP0223070.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(7909..8817)
                     /gene="ycaN"
                     /locus_tag="ACHK74_004364"
     CDS             complement(7909..8817)
                     /gene="ycaN"
                     /locus_tag="ACHK74_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0223071.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            8917..9507
                     /gene="ycaK"
                     /locus_tag="ACHK74_004365"
     CDS             8917..9507
                     /gene="ycaK"
                     /locus_tag="ACHK74_004365"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFP0223072.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(9589..10329)
                     /gene="pflA"
                     /locus_tag="ACHK74_004366"
     CDS             complement(9589..10329)
                     /gene="pflA"
                     /locus_tag="ACHK74_004366"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFP0223073.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(10521..12803)
                     /gene="pflB"
                     /locus_tag="ACHK74_004367"
     CDS             complement(10521..12803)
                     /gene="pflB"
                     /locus_tag="ACHK74_004367"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP0223074.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMARMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(12858..13715)
                     /gene="focA"
                     /locus_tag="ACHK74_004368"
     CDS             complement(12858..13715)
                     /gene="focA"
                     /locus_tag="ACHK74_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFP0223075.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         3397 a         3263 c         3576 g         3681 t
ORIGIN      
        1 cgggcgcttt ttttgtctcc ctttgatacc gaacaataat tactcctcac ttacacgtaa
       61 tactactttc gagtgaaaat ctacctatct ctttgatttt caaattattc gatgtataca
      121 agcctatata gcgagctgct atagaaataa ttacacaata cggtttgtta ctggaatcaa
      181 tcgtgagcaa gcttgagtga gccattatga aaacgaaaat ccctgatgcg gtattggctg
      241 ctgaggtgag tcgccgtggt ttggtaaaaa cgacagcgat cggcggcctg gcaatggcca
      301 gcagcgcatt aacattacct tttagtcgga ttgcgcacgc tgtcgatagc gccattccaa
      361 caaaatcaga cgaaaaggtt atctggagcg cctgtacagt taactgtggt agtcgctgcc
      421 cgctacgtat gcacgtcgtg gacggtgaaa tcaaatatgt cgaaacggac aataccggcg
      481 atgacaatta cgacggcctg caccaggttc gcgcctgcct gcgtgggcgt tccatgcgtc
      541 gccgtgtcta caatccggac cgcctgaaat atccgatgaa acgagtcggg gcgcgcggtg
      601 aaggcaaatt cgagcgcatt agctgggaag aagcctacga catcatcgcg accaatatgc
      661 agcgcctgat caaagagtac ggcaacgagt ctatctatct gaactatggc accggtacgc
      721 tgggcggcac catgacccgc tcctggccgc cgggaaatac cctggtcgcg cggctgatga
      781 actgctgcgg cggctatctg aaccattacg gcgactactc ctccgcgcaa attgcggaag
      841 gtttgaacta tacctacggc ggctgggcag atggcaacag cccgtcggat atcgaaaaca
      901 gtaagctggt agtgctgttt ggtaataacc ctggcgaaac gcgaatgagt ggcggtgggg
      961 tgacttacta tcttgaacag gcacgccaga aatctaatgc ccgcatgatc atcatcgatc
     1021 cgcgctatac cgacaccggt gccgggcgcg aagatgagtg gatccctatt cgtccgggaa
     1081 cagatgccgc actggttaac ggtctggcgt acgtcatgat cactgaaaac ctggtggatc
     1141 aggcattcct cgataaatat tgcgttggct acgatgagaa aaccctgcca gccagtgcgc
     1201 cgaaaaatgg ccactataaa gcttatattc tgggtgaagg gccagatggc gtggctaaaa
     1261 caccggaatg ggcctcacaa atcaccggtg ttccggcaga caaaatcatc aaactggctc
     1321 gtgaaatcgg cagtaccaaa ccggcgttta tcagccaggg atggggcccg cagcgtcacg
     1381 ctaacggtga aatcgcaacc cgtgctatct cgatgctggc gattctgacc ggtaacgttg
     1441 gtattaacgg aggcaacagc ggcgcgcgtg aaggttcata cagcttaccg tttgtccgta
     1501 tgccgacctt ggaaaacccg atccagacca gcatttcgat gtttatgtgg accgatgcca
     1561 ttgaacgtgg cccggaaatg acagcgctgc gtgatggtgt acgcgggaaa gataagctgg
     1621 atgtgccgat caaaatgatc tggaactatg ccggtaactg cctgattaac cagcattctg
     1681 aaatcaaccg tacccatgaa atccttcagg atgataagaa gtgcgagctg attgtggtta
     1741 tcgactgcca catgacctca tcggcgaaat atgctgacat cctgctgcct gactgcaccg
     1801 cttccgaaca gatggacttt gcactggatg catcctgcgg gaatatgtct tacgtgattt
     1861 tcaacgatca ggtgattaaa ccgcgctttg aatgtaagac catctatgaa atgaccagcg
     1921 aactggcaaa acgtcttggc gttgagcaac agtttactga aggccgtaca caggaagagt
     1981 ggatgcggca tctgtatgcc cagtcgcggg aagcgattcc tgaactgcca acgtttgaag
     2041 agttccgcaa gcaggggatc tttaaaaagc gcgacccaca agggcaccac gttgcttata
     2101 aagctttccg tgaagatcct caggcaaatc cactgaccac gccatcgggc aaaattgaga
     2161 tttattcgca ggcgctggct gacattgccg ctacctggga attgccagaa ggcgatgtaa
     2221 tcgatccact gccgatctac acgccgggct ttgaaagtta tcaggatccg ttgaacaaac
     2281 agtatccgct gcagcttacg ggtttccact ataaatctcg cgttcactca acttacggca
     2341 acgttgatgt gctgaaagca gcttgccgtc aggaaatgtg gatcaacccg cttgatgcgc
     2401 aaaaacgcgg tatcaacaac ggcgataaag tcaggatctt taacgatcgt ggtgaggttc
     2461 atattgaggc gaaagtgacg ccacgaatga tgccgggtgt ggtcgcactg ggtgaaggtg
     2521 cctggtatga cccggataca aaacgtgtcg ataagggcgg ttgtatcaac gtactgacca
     2581 ctcaacgtcc gtctcctctc gctaagggga atccgtcaca tacaaacctt gttcaggttg
     2641 aaaaggtgta aggagtaacc gatgacaacc cagtatggat tttttattga ttccagccgt
     2701 tgcaccggtt gcaaaacctg cgagctggcc tgtaaagact acaaagattt gacgccagaa
     2761 gtcagcttcc gccgcattta tgaatatgct ggcggcgact ggcaggaaga taacggtgtc
     2821 tggcaccaga acgtgtttgc ctactatctg tcgatttcat gtaaccactg cgaagatccg
     2881 gcttgtacta aagtctgccc gagcggtgcg atgcataaac gtgaagatgg ttttgttgtg
     2941 gtcgatgaag atgtgtgcat tggctgccgc tactgccata tggcttgccc gtatggcgca
     3001 ccgcaatata acgaaacgaa aggccatatg accaaatgcg atggttgtta tgaccgtgtt
     3061 gcggagggta aaaagccgat ctgtgttgaa tcttgtccgc tgcgggcgct ggatttcggc
     3121 cctatcgacg agctgcgtaa aaaacatggc gatctggcgg ccgttgcgcc gttgccgcga
     3181 gctcacttta ccaaaccgaa tattgtgatc aaacccaatg ccaatagccg cccgaccggg
     3241 gataccactg gctatctggc aaacccgaag gaggtgtgag atgggaagtg gatggcatga
     3301 atggccgctg atgatcttca cggtcttcgg gcaatgtgta gcaggtggtt ttatcgttct
     3361 ggctttggcg ctgctcaaag gcgacctgcg agcagaagcc cagcagcgtg ttatcgcctg
     3421 catgtttggt ttatgggtgc tgatgggcat tggctttatc gcctctatgc tccatcttgg
     3481 ttcaccaatg cgcgctttta actcgctcaa ccgggtaggg gcttcagcac tcagtaacga
     3541 aatcgccagc ggttcgatct tttttgccgt aggcggcatc ggctggttgc tggcaatgct
     3601 gaaaaagctg tcaccggcat tgcgtacgct gtggctgata gtgacgatgg ttcttggcgt
     3661 catctttgtc tggatgatgg tgcgtgtgta taacagcatt gataccgttc cgacctggta
     3721 cagcatctgg acgccgatgg gcttcttcct gacgatgttt atgggcggcc cgttactggg
     3781 ttacctgttg ttgagtctgg ctggcgtcga tggttgggcg atgcgtctgc tgccagcaat
     3841 ttctgtactg gcactggtag tgagtggcgt ggtgtcagtg atgcagggcg cagagctggc
     3901 gactattcat agttctgtgc agcaggccgc agcgctggtg ccggactatg gtgcgctgat
     3961 gtcctggcgg atcgtgcttt tggccgttgc cctgtgcttg tggattgcac cacagctaaa
     4021 aggttatcag cctgcggttc cgctactatc cgtctcattc attctgcttc tggcagggga
     4081 attaatcggt cgcggcgtat tctatggttt gcatatgacc gtggggatgg ccgtcgcaag
     4141 ctaatcagta acaaccgggg tttcggcccc ggttctcttt tatttctgct tcgttaacgt
     4201 gtcataactg gtcatcaaat tacgataatc aggaatgtgg ttagagaaca acgtcgccag
     4261 tccttcaata tcattacgcc agtcgcgatg cagctcgcac gccacgccga accatgtcat
     4321 caattgcgca ccagcttgcg acatgcgatc ccatgccgaa tgccgggtaa tttcattaaa
     4381 ggtccctgac gcgtcagtca cgacaaagac gtcaaaccct tcttcaatgg ctgaaagcgc
     4441 cgggaatgcc acgcaaactt cggttaccac accggcaata attaactgtt ttttacctgt
     4501 cgctttgaca gcttttacaa aatcttcgtt atcccaggcg ttaatatttc ccgggcgagc
     4561 aatgtaaggt gtatcgggga attgtgcttt cagttctgga actaatgggc cgttaggacc
     4621 ggtttcaaaa ctggtggtaa gaatagttgg taagttgaaa tacttagcca ggtcacccag
     4681 cgccagcacg ttatttttaa acttatcagg ttcgatatcc cgtacaaggg aaagtaaacc
     4741 agcctggtga tcaacaagca aaacggcagc atcattttta tcaagacgaa catacggttt
     4801 ggtcatcctc ttctcctttc gagagtcagt ggtacgtctg aaatcgatca gacgcgatgc
     4861 attgctctga aagcatagac gggaaatatg agtttgctgt gaccatgaaa tttttcgact
     4921 gaacgaagcg tatgaaattt gtgttagttc aataaaaaca atcagatgag actaatcatc
     4981 tcttcagcat tagcgttatg aatgagtaat ataaatcgat aataccgccg ctgggatatc
     5041 gcgtattttc acccattgac aatgtttttg gcggtggcat gatgcgcatg aaatttgaac
     5101 ttcctcacgg ttttaattca tgtccacgta tacccggcct gtcatgcttt tgctgtctgg
     5161 cctgcttttg ttgactctgg cgattgcggt gttaaataca ctcgtgccgc tttggctcgc
     5221 ccaggaacac atgtccacat ggcaggtagg cgttgtcagc tcatcctatt ttaccggcaa
     5281 ccttgtcggt acattgctga cagggtatgt cattaagcgc attggcttta accgcagcta
     5341 ttatctggcc tccttcattt ttgccgctgg ctgtgccggc cttggcctga tgattggatt
     5401 ctggagctgg ttggcttggc gttttgtcgc gggcgtcggc tgtgccatga tttgggtggt
     5461 tgttgagagc gcgctgatgt gcagtgggac gtcacgtaac cgtgggcgtt tgcttgctgc
     5521 gtatatgatg gtttattacg tgggaacgtt tttaggccag ttactggtca gcaaagtttc
     5581 aaccgagctg atgtccgtat tgccgtgggt tacaggtttg acgttggcag ggatcttacc
     5641 gctgttgttt acgcgtgtgc tgaatcagca ggctgaaaac catgattcga cgtcaattac
     5701 gtcaatgcta aaactccgtc aggcgcggct tggcgtgaat ggctgcatta tctcaggaat
     5761 cgttctggga tctctatatg gcctgatgcc gctgtacctc aatcacaaag gggtgagcaa
     5821 tgccagcatt ggtttctgga tggcggtact ggtcagtgcg ggtatccttg gacaatggcc
     5881 gattggacgt ctggcggata agtttggtcg actgctggtg ttgcgtgttc aggtctttgt
     5941 cgtcattctc ggcagtatcg cgatgcttag ccaggcggcg atggccccag cgttattcat
     6001 cctcggtgcc gctggcttta cgctatatcc ggtggcgatg gcatgggctt gcgagaaagt
     6061 tgaacatcat caactggtgg cgatgaacca ggccttactg ttgagctata ctgtgggaag
     6121 tctgcttggc ccgtcattta ccgctatgct aatgcagaat ttctccgata atttattgtt
     6181 tatcatgatc gccagcgtat cgtttatcta tttgctgatg ctgctgcgca acgccggtca
     6241 tacgccgaaa cccgttgctc acgtgtaaat gaattcaagc agagtgtgaa cttactgttt
     6301 cacactctgc ttttttgttt cttctatctg acttgcttta ttccaaattt tattcgttta
     6361 aaaataaaat gtgcagcagg ttataatttt gcatttcgct atttccgcac ttcttatttg
     6421 ccgcgcataa tccctcgttt tacagatgcc cctttaattt tggcgaagga tttgtctatg
     6481 gctgggaatg ttcaggaaaa acagctgcga tggtacaaca ttgcgctgat gtcttttatc
     6541 actgtctggg gttttggcaa cgttgttaat aactacgcca accaggggct ggtggttgtt
     6601 ttttcatggg tgtttatctt tgcactctat ttcacacctt atgcgctaat tgttggtcag
     6661 ttaggctcga ccttcaaaga tgggaagggc ggggtcagta cctggattaa acacacgatg
     6721 ggacccggac tggcttatct cgccgcgtgg acctactggg tggtgcatat tccctatctg
     6781 gcacaaaaac cccaggcaat tctgattgcg ctcggttggg cgatgaaagg cgacggttcg
     6841 ctaatcaaag aatattcagt cgtagcgtta caggggttaa cgctggtgct gtttatcttc
     6901 tttatgtggg ttgcttcacg cggtatgaaa tcgctgaaaa tcgtcggttc tgtggcaggg
     6961 attgcgatgt ttgttatgtc gctcctgtat gtggcgatgg cggtaaccgc gcctgcgatt
     7021 actgaagtgc atattgcgac cacaaacatt acctgggaaa cgttcattcc tcatatcgac
     7081 tttacctaca ttaccactat ttcaatgctg gttttcgcgg ttggcggagc agagaagatt
     7141 tctccttacg ttaatcaaac gcgcaaccca ggaaaagaat ttccaaaagg gatgctatgc
     7201 ctggcggtga tggttgcggt ttgtgccatt ctgggctcac tggcgatggg gatgatgttt
     7261 gactcgcgta atatcccgga tgacttaatg actaacggtc aatattacgc ctttcagaag
     7321 ctgggcgagt attacaacat gggtaatact ttaatggtga tttacgccat tgcgaatacc
     7381 ctgggacaag tagctgcgct ggtattctcg attgatgccc cgcttaaagt gctattaggt
     7441 gatgctgaca gcaaatatat tccagccagt ttatgtcgta ccaacgcttc tggtacgccc
     7501 gttaatggct attttctgac cctggtgctg gtggcaattc tgattatgct gccgactctc
     7561 ggcatcggtg atatgaacaa tctctataaa tggctgttga atcttaactc ggtagtgatg
     7621 ccgctgcgtt atttatgggt atttgttgca tttattgcag tcgttcgcct ggcgcagaaa
     7681 tataaaccag aatatgtctt tattcgtaat aagcctctgg caatgaccgt cgggatttgg
     7741 tgttttgcct ttaccgcctt tgcctgtttg acggggatct tcccgaaaat ggaagccttc
     7801 actgcagagt ggaccttcca gttggcgctg aatgttgcaa cgccgtttgt gctggtagga
     7861 ttaggactga ttttcccgct gctggcgcgt aaagcgaata gtaaataatt attgtggtcg
     7921 gtgacgctga ctctggcaga tctgacccgt ttttacataa tcgagaaatg cgcgcaagcc
     7981 gcaggacata tactggcggt ttggataata gatctggaag cctggacgct ctgtgctcca
     8041 gtctcccagt acacattcta aacgaccagt atcaaggtac tccttgatct gttcatagag
     8101 cagatacccg ataccagcac ccatcaaaac ggcctccagt tcagcatcaa catcatccag
     8161 aatgatattt cctgcgacag caatttccag ctcctttgca aattgccagt ggaaaggttt
     8221 gccgctggga taacgaaaaa caacacattg atgattaagc agatcatgag gatggcgggg
     8281 tttgccatat cttgcaaagt actctggcgt tgctgctaca cataatttca ctggtgggcc
     8341 gatcgccacg ctgatcatat ctttttcgac gatgccacta agtcgaacgc cagcgtcaaa
     8401 gccctgttgg acgatatcga caagagaatc gtcagttgtg agctcgactt taatatcagg
     8461 atattcacgt gtgaaaccaa ccagtaaagg cattaaaaat atacgtgcag ctacccgggc
     8521 ggcattgatt ttgagcgttc ctgtcggtgt cagacgaaaa tcattcatct catctaacat
     8581 gatctgaatt tcgtcgaaag ctgggcgcaa tcgttcatag agattagaac ccgcttccgt
     8641 taacgatacg cttcgggttg tccgattgaa taagcgaatt ttaagacgtt gttccagtgt
     8701 tttaatacta tggctaatgg cggacgagga taagcctaat tcatcgcccg ctgcacgaaa
     8761 gctttgatta cgggccacgg caaagaaagt ggcaaagtca gacatattca tccgcattag
     8821 tgaaaatcct tcattaactc atcctgaatc atcacgttta caggattatg ccgcctgcgt
     8881 aaagtgcgct ccagaactta acgtggaggt aaaattatgc agtctgaacg tatttatttg
     8941 gtatgggccc atcctcgtca tgattcattg accgcacata ttgctgatgc gatccatcag
     9001 cgggcaatgg agcggaaaat acaggtgacg gaactcgatt tatatcggcg taatttcaac
     9061 ccggtgatga cgtcggaaga tgaaccagac tggaagaata tggataaacg ttattctcca
     9121 gaggttcatc agctttattc agagctgctt gagcatgaca cgttagtggt ggtttttcct
     9181 ctctggtggt acagcttccc ggcaatgcta aaaggatata ttgacagagt atggaataat
     9241 gggctggctt atggagatgg gcacaaatta ccattcaata aagttcgttg ggtggcgctg
     9301 gttggaggag acaaagaatc atttgtccag atgggctggg aaaaaaatat aagcgattat
     9361 ttaaaaaata tgtgcagtta tcttggtatt gaagatgccg atgtcacttt cttgtgtaat
     9421 acagtggtat tcgatgggga agaacttcac gcgagctatt atcagtcgtt attatctcag
     9481 gtacgggata tggtagatgc actataagat gtgttaaaaa cgctgtagca gaatgaagcg
     9541 cggaataaaa aagcggcaac tcaataaagt tgccgcttta cggggaaatt agaacattac
     9601 cttatgaccg tactgctcaa gaatgccttt cacgcgttcc atggtctctt tcttcggtgg
     9661 tttaacaccg tcgagtttgt actcttcacc cattgccacc catttgtgtt tgcccagctc
     9721 gtggtagggg agaagctcga ttttctcaac gttgcccata tcacgggtaa attcaccgag
     9781 gcgatgcgct gaatcgtcat cgtcagacca gcctgggaca acaacgtagc ggatccacac
     9841 cttcacattt ttgttcgcca gatatttggc gaactccagc gtgcggtgat tggaaactcc
     9901 aaccagattt tggtggatct cgtcgttcat ctgtttgaga tcgagcatta ccaggtcggt
     9961 tacttccagc agttcatcaa tcaccggatc gtaacgacga acaaaaccgt tggtgtccag
    10021 acaggtatga atgccttctt ttttgcaggc gcggaaccag tcacgaacaa actcagcttg
    10081 taggattgcc tcaccgccgg atgcggtaac gccgccgccg gaagcgttca taaagtggcg
    10141 ataggtcacc acctctttca ttaaatcttc aacggtaact tctttaccgc catgcgtatc
    10201 ccaggtgtcg cggttatgac aatacaggca gcgcatcagg cagccctgga aaaaggtgat
    10261 aaagcgaata cctgggccgt ctacggttcc acaggattca aaggagtgaa tgcgaccaat
    10321 aactgacatt gcggtgtttc tccagatgtg gcccatctga ggccgtgttg gtgcgcagct
    10381 cgaaggctac gtcgagtctg ttttggcagt caccttaaag tatagatagc tgacaaaaaa
    10441 ggctctcgtg ctaaaaaagg ccccactttc gtggagcctt tattgtacgc tttttactgt
    10501 acgatttcag tcaaatctaa ttacatagat tgagtgaagg tacgagtaat aacgtcctgc
    10561 tgctgttctt tagtcagcga gttgaaacgt actgcgtagc cagatacacg gatggtcagc
    10621 tgcggatatt tttccgggtt ttccatcgcg tcgagcagca tttcacggtt catcacgtta
    10681 acgttcaggt gctgaccacc ttcgatggat gcttcgtggt ggaagtaacc atccatcaga
    10741 ccagccaggt tggtcttacg aacttcgtcg tctttaccca gtgcgttcgg aacgatagag
    10801 aaggtgtagg agataccatc tttagcgtaa gcaaacggca gtttagcaac ggaagtcaga
    10861 gacgctacag cacctttctg gtcacgaccg tgcatcgggt tagcacccgg tccgaacggc
    10921 gcgccagcac gacggccgtc tggggtgtta ccagttttct taccatacac aacgttagaa
    10981 gtgatggtca gaacagactg agtcgggata gcgtcacggt aggtgtgcag tttctgaatt
    11041 ttcttcatga aacgttctac caggtcaaca gccaggtcat ctacacgcgg atcattgtta
    11101 ccaaactgcg ggtattcgcc ttcgatttcg aagtcgatag ccagaccgtc ttcgtcacga
    11161 atcggtttaa ctttcgcata tttgattgca gacagggagt cagcagcaac ggacagacca
    11221 gcgataccac acgccatagt gcggataacg tcacggtcgt gcagcgccat cagagaggct
    11281 tcgtagctgt acttgtcgtg catgtagtgg atgatgttca gtgcagtgat gtactgttta
    11341 gccagccagt ccatgaagtg atccatgcgc gccatcactt catcatagtt caggacatcg
    11401 cctttgatcg gttcagactt cggaccaacc tgcattttca gtttttcgtc aacgccgccg
    11461 ttgattgcgt acagcatggt tttcgccagg tttgcacgcg caccgaagaa ctgcatttgt
    11521 ttaccaacga tcatcgggct tacgcagcaa gcaatagcgt agtcatcgtt gttgaagtcc
    11581 ggacgcatca ggtcatcgtt ctcatactgc agagaagagg tgtcgatgga cactttagcg
    11641 gcgaatttct tgaagttcag cggcagtttt tcagaccaca gaatggtcat gttcggttcc
    11701 ggagacggac ccatggtgta cagggtgttc aggaaacgga agctgttttt ggtaaccagg
    11761 gtacgaccgt cgaggcccat accaccgata gattcggttg cccagatcgg gtcgccagag
    11821 aacagttcat cgtactccgg agtacgcagg aagcgaacca tacgcagttt catgaccagg
    11881 tggtcaacca tttcctgcgc ttcttgttcg gtgatcttgc cagctttcag gtcacgttcg
    11941 atgtacacat ccaggaaggt ggaggtacga ccgaaggaca ttgcagcacc gttctgagac
    12001 ttaacagcag ccaggtagcc gaagtaagtc cactggatag cttcctgagc gttggtagcc
    12061 ggaccagaga tgtcgtagcc gtatttcgca gccatttctt tcatctgacc cagagcgcgg
    12121 tgctgttcag cgatttcttc gcgcagacgg atagtctgtt ccaggtttac gccgttttcc
    12181 agatcagcct gcagagaagt gaactgtgcc agtttgtctt tcatcaggta gtcgataccg
    12241 tacagcgcaa cgcgacggta gtcaccgatg atacggccac ggccatatgc atctggcaga
    12301 ccggtcagaa caccagattt acggcaacgc aggatgtccg gagtgtaaac gtcgaacacg
    12361 ccctggttgt gagttttacg gtattcagtg aagatttttt tgatcatcgg atccagttcg
    12421 cggttgtacg ctttgcagga accttcgatc attttgatac caccgaacgg gataagagca
    12481 cgtttcagcg gagcttcagt ctgcagacca acgattttct caagctgctt gttgatgtag
    12541 ccagcgtcgt gagaggtgat ggtggaagca acagcggtgt caaagtcaac tggcgcgtga
    12601 gtgcggtttt ccagtttaac gccttccatt actttgtccc acagggtggt ggtcgcttca
    12661 gtagcgccag ccaggaagga ctcgtcaccc tcgtacggag tgtagttttt ctgaatgaag
    12721 tcacggacgt ttacttcatt ctgccagtca cctttggtaa aaccttccca ggctgtggct
    12781 aacttttcat taagctcgga catgtaacac ctaccttctt aagtggattt tttatttact
    12841 gcgtacttcg acaaccatta atggtggtcg ttttcacgca ggtaaatgac ccagtatgtc
    12901 aacccaacca acaaaccacc accgataatg ttgccgatcg taaccggaat caggttatca
    12961 gtgatgaaat tcatcacggt caggtgagaa aaattttccg gtgcagaacc gactgcggtc
    13021 caaaattccg gggatgcgaa gtcgcggatt acaataccca tcgggatcat aaacatgttt
    13081 gcgatactgt gctcaaaacc gctggcaaca aacatcgcga ccggcagcac cataatgaac
    13141 gctttgtcca tcaggctgcg gccagaataa ctcatccata ctgccagaca taccatcagg
    13201 tttgccagga taccaagaca gacggcctca ataaaagtat ggtgcacttt gtggtcggcg
    13261 gtttgtagga cgtttagtcc ccattgacca tttgcggtca tatactcgcc ggaaagccac
    13321 attaaaagta caaacagcag tgcgccgacc aggttgccaa aatagacatt tagccagttt
    13381 ttcgccaact gaccccaggt gatgcgccca ctcgccttag caacaacaat caacacggtg
    13441 gaagtaaaga gatcggctcc gcagacaaca caaagaatca gccccagaga gaagcaaatg
    13501 ccgccaacca gttttgccat gccgaagggc attgtgcctg tgccagtggt tgctgtgata
    13561 tagaagacga atgcgattga gatgaaaaca ccggcggtaa tcgccagata gaaagtctta
    13621 agcggatgtt tcgttgcttt atagacaccc gcctcttcgg ccactttggc cattgcagca
    13681 ggaagtaaaa gatcaaaagg gttgtcagct ttcacactaa ctctctcttt attaagtcgg
    13741 cgacgagata ctaacaaagc attatagatg agaaattgat atagatcata tctcgcctgg
    13801 cttataggcc cgtaactcgc atggttttta tgcaaatacg gagtaaatat ttgattatcc
    13861 aaataaaaat aaattttaaa aattaacaaa tgagttgaat tttttccgca tcctccg
//
LOCUS       DAZGSP010000129        13828 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZGSP010000129 DAZGSP010000000
VERSION     DAZGSP010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.130"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..283
                     /gene="yoaC"
                     /locus_tag="ACHK74_004369"
     CDS             <1..283
                     /gene="yoaC"
                     /locus_tag="ACHK74_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFP0223076.1"
                     /translation="KALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPASAADIT
                     ARADQEGWNPGFTEKMVGWAKKMESGERTVIKNPEYFSTYMQEELKALV"
     gene            complement(403..582)
                     /gene="yoaH"
                     /locus_tag="ACHK74_004370"
     CDS             complement(403..582)
                     /gene="yoaH"
                     /locus_tag="ACHK74_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFP0223077.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            656..2017
                     /gene="pabB"
                     /locus_tag="ACHK74_004371"
     CDS             656..2017
                     /gene="pabB"
                     /locus_tag="ACHK74_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFP0223078.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2021..2599
                     /gene="nudL"
                     /locus_tag="ACHK74_004372"
     CDS             2021..2599
                     /gene="nudL"
                     /locus_tag="ACHK74_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFP0223079.1"
                     /translation="MEYRSLTLDYFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2783..4147
                     /gene="sdaA"
                     /locus_tag="ACHK74_004373"
     CDS             2783..4147
                     /gene="sdaA"
                     /locus_tag="ACHK74_004373"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP0223080.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4278..5876
                     /gene="pdeD"
                     /locus_tag="ACHK74_004374"
     CDS             4278..5876
                     /gene="pdeD"
                     /locus_tag="ACHK74_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0223081.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5880..7436)
                     /gene="yoaE"
                     /locus_tag="ACHK74_004375"
     CDS             complement(5880..7436)
                     /gene="yoaE"
                     /locus_tag="ACHK74_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFP0223082.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLSVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7424..7582)
                     /gene="yoaL"
                     /locus_tag="ACHK74_004376"
     CDS             complement(7424..7582)
                     /gene="yoaL"
                     /locus_tag="ACHK74_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFP0223083.1"
                     /translation="MDRHRRHFSIRPFNACLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            7899..8870
                     /gene="manX"
                     /locus_tag="ACHK74_004377"
     CDS             7899..8870
                     /gene="manX"
                     /locus_tag="ACHK74_004377"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFP0223084.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8933..9733
                     /gene="manY"
                     /locus_tag="ACHK74_004378"
     CDS             8933..9733
                     /gene="manY"
                     /locus_tag="ACHK74_004378"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFP0223085.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9746..10597
                     /gene="manZ"
                     /locus_tag="ACHK74_004379"
     CDS             9746..10597
                     /gene="manZ"
                     /locus_tag="ACHK74_004379"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFP0223086.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10652..11110
                     /gene="yobD"
                     /locus_tag="ACHK74_004380"
     CDS             10652..11110
                     /gene="yobD"
                     /locus_tag="ACHK74_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFP0223087.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            11539..12105
                     /gene="mntP"
                     /locus_tag="ACHK74_004381"
     CDS             11539..12105
                     /gene="mntP"
                     /locus_tag="ACHK74_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFP0223088.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATTLAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(12102..12911)
                     /gene="rlmA"
                     /locus_tag="ACHK74_004382"
     CDS             complement(12102..12911)
                     /gene="rlmA"
                     /locus_tag="ACHK74_004382"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFP0223089.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(13077..13286)
                     /gene="cspE"
                     /locus_tag="ACHK74_004383"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(13077..13286)
                     /gene="cspE"
                     /locus_tag="ACHK74_004383"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP0223090.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(13299..13442)
                     /gene="yobF"
                     /locus_tag="ACHK74_004384"
     CDS             complement(13299..13442)
                     /gene="yobF"
                     /locus_tag="ACHK74_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFP0223091.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
BASE COUNT         3416 a         3465 c         3439 g         3508 t
ORIGIN      
        1 taaagccctg gatttcattg gtgccatgga tgtatcagcg ccaacaccaa gttcgatgaa
       61 tgaaagcaca gcgaagggaa tctttaaata tttaaaagaa ctgggagtac ccgcgagtgc
      121 cgctgatatt acggcgcgag ctgaccagga aggctggaac cctgggttca cggaaaaaat
      181 ggttggatgg gcaaaaaaaa tggagtctgg tgaacgcact gtaattaaaa atcctgaata
      241 cttttcaaca tatatgcagg aagaactaaa agcactggtc tgagttaaat ttatatcagc
      301 ataaatgggt caggacgctt ttaatcacat ataaaaagcg tcctttttct cacaatcaaa
      361 tgatcatcgg cttctataac gaaatcgata cggattagtg tgttactcgt cttcatcctc
      421 aaaacgcgcc acgattcgct cgccggagtg gttggcacgc agctcttctg ccaccagcgc
      481 gattgcctgg ccgctgctca tcccttgtgc catcagttcc tggatccgct cgacagcttt
      541 ttgctgctgt tcatgggtga gtgaaggtaa acctgcaaac attgttaact cctgctaaat
      601 tgttggcgct aattatttca tgctacccgg cacatagcca gtagagtcag gactgatgaa
      661 gacgttatct cccgctgtga ttactttacc ctggcgtcag gacgccgctg aattttattt
      721 ctcccgctta agccacctgc cgtgggcgat gcttttacac tccggctatg ccgatcatcc
      781 gtatagccgc tttgatattg tggtcgccga tccgatttgc actttaacca ctttcggtaa
      841 agaaaccgtt gttagtgaaa gcgaaaaacg cacaacgacc actgatgacc cgctacaggt
      901 gctccagcag gtgctggatc gcgcagacat tcgcccaacg cataacgaag atttgccatt
      961 tcagggcggc gcactggggt tgtttggcta cgatctgggc cgccgttttg agtcactgcc
     1021 agaaattgcg gaacaagata tcgttctgcc ggatatggca gtgggtatct acgattgggc
     1081 gctcattgtc gaccaccagc gtcatacagt ttctttgctg agtcataatg atgtcaatgc
     1141 ccgtcgggcc tggctggaaa gccagcaatt ctcgccgcag gaagatttca cgctcacttc
     1201 cgactggcaa tccaatatga cccgcgagca gtacggcgaa aaatttcgcc aggtacagga
     1261 atatctgcac agcggtgatt gctatcaggt gaatctcgcc cagcgttttc atgcgaccta
     1321 ttctggcgat gaatggcagg cattccttca gcttaatcag gccaaccgcg cgccatttag
     1381 cgctttttta cgtcttgaac agggtgcaat tttaagcctt tcgccagagc ggtttattct
     1441 ttgtgataat agtgaaatcc agacccgccc gattaaaggc acgctaccac gcctgcccga
     1501 tcctcaggaa gatagcaaac aagcagaaaa actggcgaac tcagcgaaag atcgtgccga
     1561 aaatctgatg attgtcgatt taatgcgtaa tgatatcggt cgtgttgccg tagccggttc
     1621 ggtaaaagta ccagagctct tcgtggtgga acccttccct gccgtgcatc atctggtcag
     1681 cactataacg gcgcgactac cagaacagtt acacgccagc gatctgctgc gcgcagcttt
     1741 tcctggtggc tcaataaccg gggctccgaa agtacgggct atggaaatta tcgacgaact
     1801 ggaaccgcag cgacgtaatg cctggtgcgg cagcattggc tatttgagct tttgcggcaa
     1861 catggatacc agcattacta tccgcacgct gactgccatt aacggacaaa tatactgctc
     1921 tgcgggcggt ggaattgtcg ccgatagcca ggaagaagcg gaatatcagg aaacttttga
     1981 taaagttaat aagatattac gccaactgga gaagtaagac gtggaatacc gtagcctgac
     2041 gcttgattat tttttatctc gctttcaact tttgcgcccg caaattaacc gggaaaccct
     2101 aaatcatcgt caggctgccg tgttaatccc catcgtccgt cgaccgcaac cggggttgtt
     2161 gctgactcag cgttcaattc atctgcgtaa acacgctgga caagtggcat tccctggagg
     2221 tgcagtcgat gacacggacg catcagttat cgccgccgcg ctgcgcgaag ctgaagaaga
     2281 ggtcgctata ccgccttccg ccgttgaagt tatcggcgtg ctgccgcccg tcgatagcgt
     2341 aactggctat caggtaaccc cagtggtcgg cattatcccg cccgatctgc cgtatcgcgc
     2401 cagtgaagat gaagtctcgg cggtgtttga aatgccgctc gcccaggcat tacatctggg
     2461 tcgttatcac cctttagata tctaccgccg tggcgattca catcgggtat ggctgtcctg
     2521 gtacgaacag tattttgtat ggggaatgac cgcaggcata attcgtgagc tggcgctgca
     2581 aattggtgtg aaaccctgac tatacttatc tttacatcta caaaacacta cttgagacaa
     2641 tcatcgcaat attagttaaa tcgcggtttt tgattagttt aattcatgtg aatagttaag
     2701 ccagtcgccg cgttccctct tacactatgc gctgttatta gttcgttact ggaagtccag
     2761 tcaccttgtc aggagtatta tcgtgattag tctattcgac atgtttaagg tggggattgg
     2821 tccctcatct tcccataccg tagggcctat gaaggcgggt aaacagttcg tcgatgatct
     2881 ggtcgaaaaa ggcttactgg atagcgttac tcgcgttgcc gtggacgttt atggttcact
     2941 gtcgctgacg ggtaaaggcc accacaccga tatcgccatt attatgggtc ttgcaggtaa
     3001 cgaacctgcc accgtggata tcgacagtat tcccggtttt attcgcgacg tagaagagcg
     3061 cgaacgtctg ctgctggcac agggacggca tgaagtggat ttcccgcgcg acaacgggat
     3121 gcgttttcat aacggcaacc tgccgctgca tgaaaacggt atgcaaatcc acgcctataa
     3181 cggcgatgaa gtcgtctaca gcaaaactta ttattccatc ggcggcggtt ttatcgtcga
     3241 tgaagaacac tttggtcagg atgctgccaa cgaagtgagc gtgccgtatc cgttcaaatc
     3301 tgccaccgaa ctgctcgcgt actgtaatga aaccggctat tcgctgtctg gtctcgctat
     3361 gcagaacgaa ctggcgctgc acagcaagaa agagatcgac gagtatttcg cgcatgtctg
     3421 gcaaaccatg caggcatgta tcgatcgcgg gatgaacacc gaaggtgtac tgccaggccc
     3481 gctgcgcgtg ccacgtcgtg cgtctgccct gcgccggatg ttagtttcca gcgataaact
     3541 gtctaacgat ccgatgaatg tcattgactg ggtaaacatg tttgcgctgg cagttaacga
     3601 agaaaacgcc gccggtggtc gtgtggtaac tgcgccaacc aacggtgcct gcggtatcgt
     3661 tccggcagtg ctggcttact atgaccactt tattgaatcg gtcagcccgg acatctatac
     3721 ccgttacttt atggcagcgg gcgcgattgg tgcattgtat aaaatgaacg cctctatttc
     3781 cggtgcggaa gttggttgcc agggcgaagt gggtgttgcc tgttcaatgg ctgctgcggg
     3841 tcttgcagaa ctgctgggcg gtagcccgga acaggtttgc gtggcggcgg aaattggcat
     3901 ggaacacaac cttggtttaa cctgcgaccc ggttgcaggg caggttcagg tgccgtgcat
     3961 tgagcgtaat gccattgcct ctgtgaaggc gattaacgcc gcacggatgg ctctgcgccg
     4021 caccagtgca ccgcgcgtct cgctggataa ggtcatcgaa acgatgtacg aaaccggtaa
     4081 ggacatgaac gccaaatacc gcgaaacctc acgcggtggt ctggcaatca aagtccagtg
     4141 tgactaatac ttcttactcg cccatctgca acggatgggc gaatttatac ccgctttctc
     4201 gtctgctgta atattcccca ctacacttcc actgttgcgt caggcgtttg tcgccatacg
     4261 cttacagggt ggcccgcatg caaaaagcac aacggatcat taaaacctat cgccgtaatc
     4321 gaatgattgt ttgtacgatt tgcgcactcg ttacgctcgc ttcgaccctg agcgtgcgat
     4381 ttatttcaca gcgtaactta aatcaacaac gggtagtaca attcgccaat cacgctgtag
     4441 aggaattaga taaagtactg cttcccctac aggcaggtag cgaagtcttg cttccgctga
     4501 ttggtctgcc ctgctctgtc gcccatttgc cattacgtaa acaggcggca aaactccaaa
     4561 ctgtgcgatc cattggcctg gtgcaagacg gcacactcta ttgctccagc atttttggtt
     4621 atcgcaatgt gcccgtcgtg gacattctgg ctgaacttcc tgcaccgcaa ccacttttac
     4681 gcctgacgat cgaccgtgcc ctgattaaag gcagtccggt tttgattcaa tggacgcctg
     4741 cagcgggcag tagcaatgct ggggtcatgg agatgattaa catcgactta ctgacggcaa
     4801 tgctccttga gccacaactg ccgcaaatca gtagcgccag cctgacggtg gacaaacggc
     4861 atttgctcta tggtaatggg ctggtagatt cccttccgca acctgaagac aatgaaaact
     4921 atcaggtttc ttcgcaacgc tttcctttta ccattaacgt taatggtccg ggggctacgg
     4981 cgctggcatg gcactatctt ccaacacaat taccgctggc ggtgctgcta agtttactgg
     5041 tgggctacat cgcctggctg gcgaccgctt accggatgag cttttcccgc gaaatcaatc
     5101 tgggcctggc gcaacatgag ttcgaattgt tctgtcagcc tttgcttaat gcgcgcagcc
     5161 agcaatgtat tggtgtagag attttgctgc gctggaacaa tccgcgtcag ggctggattt
     5221 caccggatgt gtttattcct atcgcggaag aacatcattt aattgtgcca ctgacccgct
     5281 atgtgatggc agaaaccatt cgtcagcgcc atgttttccc gatgagtagt cagtttcatg
     5341 ttggcattaa cgtcgcaccc agccattttc gccgtggtgt actgataaaa gatctcaatc
     5401 agtactggtt tagcgctcac ccgattcagc aactaatcct cgaaatcacc gaacgcgatg
     5461 ccttactgga tgttgattat cggattgccc gcgagctaca tcgtaaaaac gtcaaactgg
     5521 cgattgatga cttcggcacc ggcaacagtt cattttcctg gcttgaaaca ttacgtcctg
     5581 acgtgctgaa aattgataag tcatttaccg cagctatagg ttctgacgcg gttaactcga
     5641 cggtgaccga tatcatcatc gcgctggggc aaagactgaa tattgaactg gtggcggagg
     5701 gcgtggaaac gcaagaacag gcgaagtatt tgcgccgtca tggcgtgcat attttgcaag
     5761 ggtatttgta cgcacagccg atgccgctac gtgattttcc caaatggctg gcgggcagcc
     5821 aaccgccgcc cgcccggcat aatggacata tcacgcccat tatgccgtta cgttaacgct
     5881 tactcatctt catcgtgcgc cggttgctct ttaacaatgc gaaccagatc aacacgataa
     5941 tcattggctt caatgatggt gatatgcagt ggccctacat caatcacatc gcccacacgg
     6001 ggaatgtgac catttgccga gatcaccagg cccgcgaccg tcgcgatatc atcgtcatcg
     6061 gcaaggtgct caacatcaag cgcctgctgc aaggcatgca aatctgtacc gccttttacc
     6121 agccagccgt caccatcggt aatgatttcc ggcgtttcgt cagcgtccgg gaattcaccc
     6181 gcaatggctt ccagcacatc cagcggcgtg accaagcctt gtaccacacc aaactcgttg
     6241 gtcacgataa caaagctccc gcgagcgcga cgcagcacgc ccagcaggtt gatcggatcg
     6301 agggtttccg ggacgataat cgccggagac gccgaagcaa tcgccgccac atcaacgccc
     6361 tcttccagcg ccaccagcag ttctttagca cgtacgattc cgatgatttc atccagttca
     6421 ccgcgacata ccgggaacag gctgtgcggt gaggagagca gctgctcgcg gatttcatcg
     6481 accccgagat tagcgtcaac ccagcttatt tcaccgcgcg gcgtcatgat cccgcgcaga
     6541 gaacgcgacg ccagcgtcag tacgccgtta atcatgtaac gttcttcttc ggcaaatgca
     6601 ccttccggga tcggcatcgg catcgggtta tcggcatcgt gctgaacatt ggcctgacgt
     6661 ttcccgccca tcaaacgcag gatggcatcg gcagtacgcg ctcgcagcgg caaagtcgac
     6721 tggtgacgaa taaagttgcg acgcgcaatc tggttaaaca cttcgatgat gatcgagaag
     6781 ccaatcgcgg catacaggta acctttcgga atgtggaaac cgaaaccttc tgccaccaga
     6841 ctcagaccaa tcattaacag gaagctcaga cagagcacca ccaccgtggg gtgctggtta
     6901 acgaatcgcg tcagcggttt ggatgccagc aacataaccg ccatcgcaat cactaccgcc
     6961 gccatcatca ccgacagatg gttaaccatc cctactgcag taattaccgc atccaacgag
     7021 aagacggcgt caaggatgac aatctgtgtg acgaccaccc agaaactggc gtagccttta
     7081 ccgtggccgg aatcatgatc gcggttttcc agccgttcat gcagttcggt tgttgctttg
     7141 aacagcaaga atatcccccc gaacaacata atcaggtcgc gtccggagaa ggagaaatcc
     7201 atgacggtaa atagcggttt ggtcagcgtg accatccatg aaatcagcga cagcagcccc
     7261 agacgcataa tcagcgccag tgataacccc agcaaacgcg ctttatcgcg ttgttttggc
     7321 ggcagtttgt cagcaagaat ggcgatgaag accaggttat cgatacccag cacaatttcg
     7381 agaacaacaa gcgtgagtag ccccgcccaa attgaggggt ccattaagaa ttccatgaca
     7441 agctcctgct taaggaatag ctattcgacg ccagaaataa tgcaggcgta acgacaaaat
     7501 gcaaacgaaa ggtgcggcat agagagccag aaaggcaggc gttaaaaggc ctgatgctga
     7561 aatgacgtcg gtgacgatcc atactgcggg ctactgccct atactccatg gttgttaaac
     7621 gggagttaaa catatcagag acgcctctga tttggcaaag atttaccttc ctttgcaaac
     7681 gaatgtgaca aggatatttt acctttcgaa atttctgcta atcgaaagtt aaattacgga
     7741 tcttcatcac ataaaataat tttttcgata tctaaaataa atcgcgaaac gcaggggttt
     7801 ttggttgtag cccttatctg aatcgattcg attgtggacg acgattcaaa aatacatctg
     7861 gcacgttgag gtgttaacga taataaagga ggtagcaagt gaccattgct attgttatag
     7921 gcacacatgg ttgggctgca gagcagttgc ttaaaacggc agaaatgctg ttaggcgagc
     7981 aggaaaacgt cggctggatc gatttcgttc caggtgaaaa tgccgaaacg ctgattgaaa
     8041 agtacaacgc tcagttggca aaactcgaca ccactaaagg cgtgctgttt ctcgttgata
     8101 catggggagg cagcccgttc aatgctgcca gccgcattgt cgtcgacaaa gagcattatg
     8161 aagtcattgc aggcgttaac attccaatgc tcgtggaaac gttaatggcc cgtgatgatg
     8221 acccaagctt tgatgaactg gtggcactgg cagtagaaac aggccgtgaa ggcgtgaaag
     8281 ctctgaaagc caaaccggtt gaaaaagccg cgccagcacc cgctgccgca gcaccaaaag
     8341 cggctccaac tccggcaaaa ccaatggggc caaacgacta catggttatt ggccttgcgc
     8401 gtatcgacga ccgtctgatt cacggtcagg tcgccacccg ctggaccaaa gaaaccaatg
     8461 tctcccgtat tattgttgtt agtgatgaag tggctgcgga taccgttcgt aagacactgc
     8521 tcacccaggt tgcacctccg ggcgtaacag cacacgtagt tgatgttgcc aaaatgattc
     8581 gcgtctacaa caacccgaaa tatgctggcg aacgcgtaat gctgttattt accaacccaa
     8641 cagatgtaga gcgtctcgtt gaaggcggcg tgaaaatcac ctctgttaac gtcggtggta
     8701 tggcattccg tcagggtaaa acccaggtga ataacgcggt ttcggttgat gaaaaagata
     8761 tcgaggcgtt caagaaactg aatgcgcgcg gtattgagct ggaagtccgt aaggtttcca
     8821 ccgatccgaa actgaaaatg atggatctga tcagcaaaat cgataagtaa cgtattgtgt
     8881 tgattatcac tcagttttca cacttaagtc ttacgtaaac aggagaagta caatggagat
     8941 taccactctt caaattgtgc tggtatttat cgtagcctgt atcgcaggta tgggatcaat
     9001 cctcgatgaa tttcagtttc accgtccgct aatcgcgtgt accctggtgg gtatcgttct
     9061 tggggatatg aaaaccggta ttattatcgg tggtacgctg gaaatgatcg cgctgggctg
     9121 gatgaacatc ggtgctgcag ttgcgcctga cgccgctctg gcttctatca tttctaccat
     9181 tctggttatc gcaggtcatc agagcattgg tgcaggtatc gcactggcaa tccctctggc
     9241 cgctgcgggc caggtactga ccatcatcgt tcgtactatt accgttgctt tccagcacgc
     9301 tgcggataag gctgctgata acggcaacct gacagcgatt tcctggatcc acgtttcttc
     9361 tctgttcctg caagcaatgc gtgtggctat tccggccgtc atcgttgcgc tgtctgttgg
     9421 taccagcgaa gtacagaaca tgctgaatgc gattccggaa gtggtgacca atggtctgaa
     9481 tatcgccggt ggcatgatcg tggtggttgg ttatgcgatg gttatcaaca tgatgcgtgc
     9541 tggctacctg atgccgttct tctacctcgg cttcgtaacc gcagcattca ccaactttaa
     9601 cctggttgct ctgggtgtga ttggtactgt tatggcagtg ctctacatcc aacttagccc
     9661 gaaatacaac cgcgtagccg gtgcgcctgc tcaggcagct ggtaacaacg atctcgataa
     9721 cgaactggac taacaggtga gcgaaatggt tgatacaact caaactacca ccgagaaaaa
     9781 actcactcaa agtgatattc gtggcgtctt cctgcgttct aacctcttcc agggttcatg
     9841 gaacttcgaa cgtatgcagg cactgggttt ctgcttctct atggtaccgg caattcgtcg
     9901 cctctaccct gagaacaacg aagctcgtaa acaagctatt cgccgtcacc tggagttctt
     9961 taacacccag ccgttcgtgg ctgcgccgat tctcggcgta accctggcgc tggaagaaca
    10021 gcgtgctaat ggcgcagaga tcgacgacgg tgctatcaac ggtatcaaag tcggtttgat
    10081 ggggccgctg gctggtgtag gcgacccgat cttctgggga accgtacgtc cggtatttgc
    10141 agcactgggt gccggtatcg cgatgagcgg cagcctgtta ggtccgctgc tgttcttcat
    10201 cctgtttaac ctggtgcgtc tggcaacccg ttactacggc gtagcgtatg gttactccaa
    10261 aggtatcgat atcgttaaag atatgggtgg tggcttcctg caaaaactga cggaaggggc
    10321 gtctatcctc ggcctgtttg tcatgggggc attggttaac aagtggacac atgtcaacat
    10381 cccgctggtt gtctctcgca ttactgacca gacgggcaaa gaacacgtta ctactgtcca
    10441 gactattctg gaccagttaa tgccaggcct ggtaccactg ctgctgacct ttgcttgtat
    10501 gtggctactg cgcaaaaaag ttaacccgct gtggatcatc gttggcttct tcgtcatcgg
    10561 tatcgctggt tacgcttgcg gcctgctggg actgtaagac tgttgtacac taccggggcc
    10621 ttttggcccc gtttttttat ctggaggatt aatgacaatc acggacctgg tactgattct
    10681 tttcatcgcc gcactcctgg ccttcgcgat ctacgatcag ttcatcatgc cccgccgtaa
    10741 cggccccacc ctgctggcaa ttcctttgct ccggcgtggt cgcatcgata gcgttatctt
    10801 cgtcggattg attgtcattc ttatctataa caacgtcacg aatcatggtg cgttaataac
    10861 gacatggtta ttaagcgcac tggctctgat gggtttttat atattctgga tccgcattcc
    10921 gaagatcatc tttaaacaaa aaggtttttt cttcgccaat gtctggattg aatatagccg
    10981 aatcaaagcg atgaacttgt cggaagatgg cgtgttggtg atgcaattag aacagcgtcg
    11041 gctgttaatc cgcgttcgaa atatcgacga tctggaaaaa atttataagc ttctcgtttc
    11101 aactcaataa gttatgaatt tagccaaagc tatgtttagt gtatttttaa taatcagaca
    11161 tagcttaggc tatattacct cttcccttat ttgttattta ttttaacgtt tcattgatat
    11221 ataaatctaa atgaaaatcg ttatcaataa agcaatgaaa taatatattc caacagttgt
    11281 tttatattct caaaatatgt taaggttgcg ccctcatttg gggagtagcc gatttccaga
    11341 ttccggaaat gtacgtgtca acatactcgt tgcaaaacgt ggcacgtacg gactgaatac
    11401 tttcagtcag gcgagaccat atgcacatca atcgctatgc ctgcatgaac gcgaaaacca
    11461 tcacggcttg ttgttcatgt atgcatgttt gctgggggcg atgatgtgtt ttatggatac
    11521 cccggtcagg acattgtcat gaatatcact gctactgttc ttcttgcgtt tggtatgtcg
    11581 atggatgcat ttgctgcatc aatcggtaaa ggtgccaccc tccataaacc gaaattttct
    11641 gaagcattgc gaaccggcct tatttttggt gccgtcgaaa ccctgacgcc gctgatcggc
    11701 tggggaatgg gcatgttagc cagccggttt gtccttgaat ggaaccactg gattgcgttt
    11761 gtgctgctga tattcctcgg cgggcgaatg attattgagg gttttcgtgg cgcagatgat
    11821 gaagatgaag agccgcgccg tcggcacggt ttctggctac tggtaaccac cgcgattgcc
    11881 accagcctgg atgccatggc tgtgggtgtt ggtcttgctt tcctgcaggt caacattatc
    11941 gcgaccacat tggccattgg ttgtgcaacc ttgattatgt cgacattagg gatgatggtt
    12001 ggtcgcttta tcggctcaat tattgggaaa aaagcggaaa ttctcggcgg gctggtgctg
    12061 atcggcatcg gcgtccagat cctctggacg cacttccacg gttaataaga acgctgccag
    12121 aggtgaatat taaagtccgt ctggcagtcg aacacttctt ttgctgccag tgtttgccag
    12181 acttctggct tcgcacgcca ggcaaacggc gtcatctgca ataatgcgac ggcttcatca
    12241 ccgcgaagac gcatcggata acacaactca tcactctgct gtaatgtaaa accttccagt
    12301 tgttctgcat gaggtgcatg aagatgtact tcattgtaaa tcagcccctt cagctccatc
    12361 aaatgtcgcg gtcccggcgt ggcagtaatg acccagccgc cgggcttcac tactcgtgct
    12421 aattcttctg ctttacacgg cgcgtaaata cgtattatgg cgtccatact ggtatcggaa
    12481 aacggcaaac ggtggctgga agcgacacaa agagtgacct gcggatagcg tttcgccgcc
    12541 gcttttatcg ctaccttcga gacatccagg ccaaacgtgg tgatttcggg caacacatcg
    12601 gcaaatgcgt gtgtgtaata cccttcacca cagccaatat ccagcaccgc cgtggccttt
    12661 tcatcaagcc gttcccgcag ttgggcgaca attgcatcac gcagcggctg ataatgtccg
    12721 gcatctaaga atgcgcggcg tgcttgtatc atttccgcgc tgtcgcccgg atcacgagac
    12781 cgtttatgct gaacgggcag cagattgaca tacccttctt tcgccatatc aaactgatgt
    12841 cgctggggac agatatagct gtttttttca cgcgaaagag gctgatggca aagtggacag
    12901 gaaaacgaca tgacaactcc ggcaggatac taaaggccgc aagtgtaacg cgaatcacgc
    12961 ggcgggcaaa tatccgaacg ccaggagcga agataaagca tagtaaaaag cctcgcattc
    13021 gacgaggctt tatagcgaga ttgaagcgta ttcacacttc agatcagtgg attcgatcag
    13081 atagctgtta cgttaacagc tgccggacct ttctggccgt cctgaatttc gaactcaacg
    13141 ttctgacctt cagccagagt tttgaagcca ttaccctgga tagcggagaa gtgtacgaac
    13201 acatctttgc tgccatcagc cggagtaatg aagccaaaac ctttagactc gttgaaccac
    13261 ttaacctgac ctttaatctt tgccatttga aaaattcctt agattgtttt cttcgcccgc
    13321 aggcataaca tagataaaac tgacacatta ctgcatgagg caccaatata aggctcggca
    13381 gagaagcggt attcaacgtc aacgtgttta ctcaggactt ctttactgaa aatgccacac
    13441 ataaacagaa ctgtacctcg tttaacccga aatctgttat cacatacaac gttaattatg
    13501 gcaagccatt tttaaacatg tctcgatcag acgcacaaat ctcgatacgt cttccacttt
    13561 ttttgcacac ttatgcaacg gaatacgcgc cgatatatca ttgtgcttaa ccttgccagt
    13621 tcaggcagat acttaaactg gcgtattttc taacatagtt caataagttc agttgttcca
    13681 ggccagtggg agaagttatt acatagtgcg tgcaatatca cattttttgc tatgcaatga
    13741 ataaaaagtt atatcacttt ttctcataaa acagtcagtt aacggctatc aattacccta
    13801 aagagaagtc aatccccaaa gggattga
//
LOCUS       DAZGSP010000130        13752 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZGSP010000130 DAZGSP010000000
VERSION     DAZGSP010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.131"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(66..3320)
                     /locus_tag="ACHK74_004385"
     CDS             complement(66..3320)
                     /locus_tag="ACHK74_004385"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFP0223092.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LHHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKTFCRWLANGFLFKRDDRQQ
                     KDLYIELLDTRHLPAALCQIFDAEDVPLQQAAELLPSYINPPLNLIKIVNQLKISGKD
                     NQSRIPDGILYINGLPLVVFEFKSAVREQDASIGNAWRQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKARHEVKICCRYPQYYAARKLYYSIKQARKPFGSGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTIVPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERSNIICISDEAHRSQVNLDQKVIVDKESGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTEAVQDEITVRIVY
                     EGRAAKVILDSSKLEEVEKYYEECANAGTNEWQIDESKKATATMNAVLGDEDRLKALA
                     EDFAKHYEKRVAEGSTVKGKAMFVCASREIAWDFYRQLKAIRPAWFEVKQAPDGVFLT
                     EQEQKELPPSEMVKMVMTRGKDDDEALYDLLGTKEYRKELDKQFKNAKSNFKIAIVVD
                     MWLTGFDVPELDTIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQAL
                     AMYSRIDATNFEDIQQSVTEVKNHLDLLGQVFYDFDSRDYFSGEPQAQLSCLNRAAEF
                     VLRTQKVERRFMGLVKRMKAAYDVCCGSEALSQTERDHIHYYLAVRSIVFKLTKGDAP
                     DVTQMNARVREMIAEALKADGVEEIYFLGDKKAESIDIFDEDYLARINKIKLPATKIQ
                     LLQKLLEKAISDFRKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEMTD
                     IIYDIKTEMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVDNT
                     SKYPDWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKKNHMS"
     gene            complement(3415..4794)
                     /locus_tag="ACHK74_004386"
     CDS             complement(3415..4794)
                     /locus_tag="ACHK74_004386"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP0223093.1"
                     /translation="MGSNYIEMRLEDCMDAIIDYRGKTPKKVDNGIPLITAKIIKNGR
                     IHEVNEFIAIDDYDDWMVRGLPLEGDVVLTTEAPLGEVAQLDARKVALAQRVITLRGK
                     KGILENDYLLYLLQSSFVQNQLDGRASGSTVTGIKQSELREIILRLPPVSLQKSISHQ
                     LKCLDKKIDLNNKINKTLEQMSQTLFKSWFVDFDPVIDNALDAGNPIQETLQTRAKLR
                     QKVRNSADFKPLPAEIRSLFPSEFEETELGWVPKGWNISPAGEEFIIKGGSTPSTANE
                     DFWINGTIHWTSPKDLTGNQSKVLLDTAKKITPLGLTKISSGLLPVNTVLMSSRAPIG
                     YLAISKVPMAINQGYIAIPSAKHLSPEYTIYWLDNIMDDIKGMAGGTTFAEISKSAFK
                     TIKLLIPSSDIITEFSRITRNHFDMVVQNTKEIDSLTNLRDTLLPKLISGELSLEDLP
                     DLSTDTEAA"
     gene            complement(4784..6403)
                     /locus_tag="ACHK74_004387"
     CDS             complement(4784..6403)
                     /locus_tag="ACHK74_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP0223094.1"
                     /translation="MARIPTKKAKAKKGFEETLWDAANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFEARRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNAKQDDIAV
                     RIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPADTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTKDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTAEDIATIADTYHAWRSTPEELA
                     ARIARGDRKLEKYEDQAGFCKVAPLQDIKNNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMQQAEELDRAIRQNLEALGYGE"
     gene            complement(6740..7378)
                     /locus_tag="ACHK74_004388"
     CDS             complement(6740..7378)
                     /locus_tag="ACHK74_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0223095.1"
                     /translation="MRTRIDYLADKYSFTELNERPHLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLIRTPQQIAALREEWDISQKNVLFNDKRFGCVYSLKS
                     SLSGVPDTYRYHLSHRIRRVVANENTSLPYQQIAREVKAPRERLKYALETGLQVTALD
                     GLFWFGSQRIAADIQRLRKSGMRITTSAISVYDSITATRRLFPVYRIVANNK"
     gene            complement(7467..7976)
                     /locus_tag="ACHK74_004389"
                     /pseudo
     CDS             complement(7467..7976)
                     /locus_tag="ACHK74_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8229..8431)
                     /locus_tag="ACHK74_004390"
                     /pseudo
     CDS             complement(8229..8431)
                     /locus_tag="ACHK74_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245245.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
     gene            complement(9072..9497)
                     /locus_tag="ACHK74_004391"
     CDS             complement(9072..9497)
                     /locus_tag="ACHK74_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP0223096.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRHCFCLSCRGRLILQTG
                     AQGAWFEHDLHALTAQQKTDCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFLDCGGAST"
     gene            complement(9494..9877)
                     /locus_tag="ACHK74_004392"
     CDS             complement(9494..9877)
                     /locus_tag="ACHK74_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP0223097.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDALPVVRKASWYCRQC
                     HHDYYGERYCTHCQTGRFSEEGVAE"
     gene            complement(10250..10849)
                     /locus_tag="ACHK74_004393"
     CDS             complement(10250..10849)
                     /locus_tag="ACHK74_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP0223098.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
     gene            12105..12896
                     /locus_tag="ACHK74_004394"
     CDS             12105..12896
                     /locus_tag="ACHK74_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223099.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQKYP
                     DVYCWVPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            13282..13488
                     /locus_tag="ACHK74_004395"
     CDS             13282..13488
                     /locus_tag="ACHK74_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0223100.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKDGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            13574..>13752
                     /locus_tag="ACHK74_004396"
     CDS             13574..>13752
                     /locus_tag="ACHK74_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223101.1"
                     /translation="MQKLENRCELLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAP"
BASE COUNT         3537 a         3284 c         3119 g         3812 t
ORIGIN      
        1 ctattttggc tcttacaaaa tagataaaga aggagtatct gatgagatac tccattatga
       61 cagactcaac tcatgtgatt tttcttaaag ttctctgctt gcgccagtac ccccatataa
      121 acatcatcat tcgctactgg cgggaactta tgcttgtgta gaagcagaat aagttcaact
      181 ttcagtttcg ctttaatatc atcgcgttta ctccagtcag gatatttcga tgtgttgtca
      241 accacgcttt tcatctcttt tgccagcgac agcatttttt catcgtcata ggtgaactga
      301 tatttatcgc gcatatgagc aagaatgtcg aagaacgctt tttcttcaat atcaatacct
      361 aaatcggccc aggtgcccat ttctgtttta atatcataga taatatcggt catttcctga
      421 ctgaatgtat cgaattcttc accgttgagt acatcatctt ctcgccgctc attataacga
      481 tctataatcg cctggaagcg gcgggtgaag ttaatccctt gcaactggtt cactttcctg
      541 aagtcgctga tcgctttttc cagtaatttt tgtaatagct ggatcttcgt tgccggaagt
      601 ttgatcttgt taattcgcgc cagataatct tcgtcaaaaa tatcgatgga ttccgctttt
      661 ttatcgccaa gaaaataaat ttcttctacg ccatcagctt ttagcgcttc tgcaatcatt
      721 tcacgaacgc gtgcattcat ctgggtaaca tccggtgcgt cacctttcgt cagtttgaaa
      781 acaattgaac gaacagcaag ataatagtga atatgatcac gttctgtctg tgatagtgct
      841 tcactgccgc agcagacgtc gtaggcggct ttcatgcgtt taaccagtcc cataaaacga
      901 cgttcaactt tctgggtacg cagaacgaat tccgccgcgc ggttgaggca ggataattgc
      961 gcttgtggct caccactaaa ataatcccga ctgtcaaagt cgtaaaagac ttgccccaac
     1021 aaatcgagat ggtttttaac ttctgtcacc gattgctgaa tatcttcaaa gttggtggca
     1081 tcaatgcggg aatacattgc cagtgcctgg ttcatctgac ttttaatgcc gatgtagtcc
     1141 accactaacc ctttgctttt gccttccagt ttacggttaa cgcgagaaat agtctggata
     1201 aggttatgtt tttgtaaggg cttatcaata tagatggtat ccagttcagg aacatcaaaa
     1261 ccggtcagcc acatatcaac aacaatggca attttgaaat tcgatttagc gtttttgaac
     1321 tgcttatcca gctctttgcg atattctttt gtgcccagta aatcataaag cgcctcgtcg
     1381 tcatctttac cgcgcgtcat gaccatcttc accatttcag aaggcggtaa ctctttttgc
     1441 tcctgttctg tcaggaagac gccatcgggg gcttgcttca cttcaaacca ggcagggcga
     1501 atagctttaa gctggcggta gaaatcccag gcaatttcac ggctggcgca aacaaacatg
     1561 gctttgcctt ttacggtgga accttcggct acgcgttttt cataatgttt ggcaaaatct
     1621 tccgcgaggg cttttaatcg atcttcatca cccagaaccg cattcatggt tgcggtggct
     1681 tttttgcttt cgtcgatttg ccactcattg gtgcccgcgt ttgcgcactc ttcgtaatac
     1741 ttttcgactt cctccagttt gctggagtcc aggatcactt tagccgcacg gccttcgtac
     1801 acgatgcgta cagtgatttc atcctgaacg gcttcggtca tggtgtagct gtcgatcacc
     1861 tcaccgaaga catcgagcgt cgcgtcaatc ggtgtgccgg taaagccaac ataggtggcg
     1921 tttggcagtg aatcgtgcag gtatttcgca aagccataag ttttgcgcac ttttccgctt
     1981 tctttatcga cgatgacttt ctggtcgagg ttaacctggc tgcgatgtgc ttcgtccgag
     2041 atgcaaatga tattgctgcg ttcagaaagg agttcggtgt cttcggtgaa tttatggatc
     2101 gttgtcagga atacaccgcc actattgcgt cccgccagtt gattacgcaa atcttcacgg
     2161 ctggtaacag gaacgatggt gtcgtcgcca atgtaatttt tggcgttgca catttgcgca
     2221 gaaagctgat cgtccagatc ggtgcggtcg gtgatcaaaa caatggtcgg gctggcaaac
     2281 tccacgcttt tcattaacag gcgggttaaa aattgcatgg tgtagctttt accgcagccc
     2341 gttgcgccaa agtaggtacc gcctttaccg ctaccgaaag gtttacgcgc ttgcttgatg
     2401 ctgtaataga gtttgcgggc ggcatagtac tgcggatatc ggcagcaaat ttttacttcg
     2461 tgcctggctt tatccgggaa gcagataaag tttttaatca catccagcag acgtaccgga
     2521 tgaaacagcc cctgaatcat tgagtgcaat gatggaattc cgtcctgttc acggttttca
     2581 ttaccggtga ctttgcgcca tgagtaaaag tattcatagg gcgcaaacag gttgcccatc
     2641 cggttattaa ctccatcgct aataatgcag agcgcgttgt agataaacag ttgtggaata
     2701 tcccggcgat agcgtttgca gagttgtctc caggcattgc caatactggc atcctgctcg
     2761 cgcaccgcac ttttaaattc aaagacgacc agtggcagac cgttgatata gagaatgccg
     2821 tcaggaatac gactctgatt atctttgccg gagattttaa gctgattaac aatcttaatc
     2881 aggttaagtg gcgggttaat ataggagggc agaagttccg ccgcctgttg caacgggaca
     2941 tcttcggcat caaatatctg gcacagtgcg gcaggtagat gccgggtgtc gagcaattca
     3001 atatagagat ctttttgttg ccgatcgtcg cgtttgaaca gaaaaccatt tgccagccag
     3061 cgacaaaatg ttttgttgct ttcataaaga tcggaagccg gaagcgtggt gaactgtttg
     3121 atcagtcgct gaatctcttc ttcagtaatg ccatcaggct ggtagcgtgc cgctaaataa
     3181 tgatggagat cgtcttcgat aatgacctga tcgagactcg aacgtgggac attatcgccg
     3241 atcaggtgtt gatatccctg atcctgtaac agttcaatga tcgcttgttc taattttgct
     3301 tcactgaaag agagactcat atcgcgctcc aaccctggct aatttctggt aagcattccc
     3361 gtatcagtag aaaaccttac cagaaagccc ctgattttac aggggcaaaa tgcgttatgc
     3421 agcttctgta tcggtgctga gatccggaag atcttccagg gatagctcac cggagatgag
     3481 tttcgggagc agggtgtcgc ggaggttggt tagtgagtct atctcttttg tattttgaac
     3541 aaccatatca aagtggttgc gagttatacg tgaaaactcg gtaattatat ccgaagaagg
     3601 aataagtaat ttaattgttt taaaagctga tttacttatt tctgcaaaag tcgttcctcc
     3661 ggccatacct ttgatatcat ccataatatt gtcaagccag tagatggtat actctggtga
     3721 taaatgttta gcagaaggaa ttgcaatata tccttgattt atagccattg gtactttact
     3781 gatcgctaaa tatccaatag gagctcgtga tgacatcaat actgtattta caggtaaaag
     3841 ccctgatgat attttggtta gtcctaatgg cgtaatcttt ttagccgtat ctagcaaaac
     3901 tttactttga tttccagtca aatcttttgg tgaagtccag tgaatagttc cattaatcca
     3961 aaaatcttca ttggctgtac ttggtgttga accaccttta ataataaact cttcaccagc
     4021 cggggatata ttccagccct ttggcaccca acccaactcc gtttcttcaa attcacttgg
     4081 gaaaagcgag cggatttccg ccggaagtgg tttaaaatct gcactattac gtactttttg
     4141 acgcaacttg gcgcgagttt gcagggtttc ctggattgga tttcctgcat ccagagcgtt
     4201 atcaatcacc ggatcaaaat ccacaaacca ggatttgaac agggtttggg acatttgttc
     4261 taaggtttta ttaattttat tatttaaatc aattttttta tcaagacact ttaattgatg
     4321 agaaattgat ttttgtaaag agactggagg cagtcttagt attatttctc gaagttcaga
     4381 ctgtttaata cctgtaacag tgcttccgga ggctcttcca tcaagctggt tttggacaaa
     4441 agaagactgc aatagatata aaaggtaatc attttctagg attccttttt taccccttag
     4501 agtaataact ctctgagcca atgctacttt gcgtgcatct aattgtgcta cctcacctag
     4561 tggtgcctct gtagttagca ctacgtcgcc ttccaatggt aacccacgaa ccatccagtc
     4621 atcataatca tcaatagcaa taaattcatt tacttcgtga attctgccat ttttaattat
     4681 ttttgccgtg attaagggga tgccgttatc aacttttttt ggggttttac ccctgtaatc
     4741 aataatagcg tccatacaat cttcaagacg catttctata taattactcc ccataaccca
     4801 gcgcctccag attctggcga atcgcacgat ccagttcttc cgcctgctgc atctgctcaa
     4861 acaacgtctt cgacaattcg cgcatttttg tctcaaatgc cacgccgtcc tcttcctgct
     4921 cggctgcacc cacatagcgg cccggcgtca gcacgtagtc gttattttta atatcttgca
     4981 ggggcgcaac tttgcagaag cctgcctggt cttcatattt ttccagcttt ctatcaccac
     5041 gcgcaatccg tgcagccagt tcttctggcg tactgcgcca ggcatggtag gtatcggcta
     5101 ttgtggcaat atcttccgcc gttaattctt tagttgtgcg gctaatcatg gtgccgaggt
     5161 tacgcgcatc aataaacagc gtctcgccct gacgatcacg ataacctttg gccggatcgg
     5221 cagccttcga tttggtcata aaccataaac acaccgggat ctgcgtggtg taaaacaact
     5281 gacctggcag agcaatcatg cagtcgatca gatcattttc gatcatctgt gcacggatct
     5341 cgccttcacc gctggtgtta gaactcatcg aaccgtttgc cagtacaaaa cctgcggtac
     5401 cgttagccga cagcttggag agcatatgca aaatccagcc gtagttggcg ttaccggttg
     5461 gcggcatacg ataaccggca aaacgtgggt ctttggttaa ttcggcttca ttgcgccagt
     5521 ctttcaggtt gaacggcggg ttcgccagaa tgtagtcggc tttcaggtcc gggtgctggt
     5581 cgctaaagaa ggtgtctgcc gggcgttcgc cgaggttagc tgaaaggccg cgaatggcga
     5641 ggttcatttt tgccagttta tacgtcgtgg ctgtcaattc ctgaccatac agcgcgatat
     5701 cacgactttt accctgatgg ctctcgacaa atttcaccga ctgcacgaac atccccgccg
     5761 aaccgcagca cgggtcataa attttgccct gaaagggttc gagcatttca gttaacagcg
     5821 taaccacaca tttgggcgtg tagaactcac ccccgccttt gccttcagtt gcggcaaact
     5881 taccgaggaa gtattcataa acgcgtccga ccaggtcttc tttcgataac gtttcaacgt
     5941 cagtctcgtg tgccagcgtt tcgatgttgt cgatggtatc aatcagtgat gccagttttt
     6001 tggtttccag attctgacgg ctgaagtagt tgtctggcag cgcacctttc agggttgggt
     6061 tacgtttctc aatggtcgag agggcggtgt caatacggac cgcaatatcg tcctgtttgg
     6121 cattttgttt gataaacgac cagcgcgcct cttccggcag gtagaaaatg ttgtcctgct
     6181 ggtagaacac ttccatctca aggaagtctg cttgcccatc ggcaatcatt tttttgcggc
     6241 gtgcttcaaa cttatcgctg atgaatttca ggaatacgag gctcaacacc acgtgcttgt
     6301 attcggagga ctcaacgctg ccgcgaagct gatttgcagc atcccataat gtttcttcaa
     6361 accctttttt tgctttagct tttttggttg gtattctggc catactgaat ccggttttct
     6421 gaacagactg gctgttattg taacgagtaa gccataaaag tgtgcatttt actctcgatg
     6481 agcattaact tcttgctgcg ttagcttgtt gcttttgttg gagtatcgcg actcaatcca
     6541 gagattagcc agaggtgagg aaccccgggt aagcactccc ggaaaaccat cgtgtaaagt
     6601 tcgcctccct gttcgataga tgattattgt ggattttccc cttactctca cttcacgtat
     6661 ccacaatccc acttctgcgc tgttcagtct gctctggtga gtcatttgca atggtaatgg
     6721 agaaactcag cattaccgtt tatttattat tcgcgacaat ccggtatacc gggaacaatc
     6781 tccgggttgc ggtaatagag tcatacacgc ttatggcaga agtggtaatc ctcataccag
     6841 attttctcag tctctgtata tctgcagcaa tgcgctggct accgaaccag aacaggccgt
     6901 ccagtgcggt cacctgcaat cccgtctcca gcgcatactt cagtcgttcc cggggggctt
     6961 tcacttcccg ggcaatctgc tgatatggca atgatgtgtt ttcattcgca accacacgac
     7021 gaatacggtg agacagatga taccggtatg tatccggtac accagaaaga ctggacttca
     7081 ggctgtacac gcaaccaaac cgtttatcgt taaacagcac atttttctgg ctgatgtccc
     7141 attcttcacg caacgctgca atctgttgtg gtgtacggat aagcagcaac cgaaatggca
     7201 gttcaaaact ggtgacgtaa tccacattca acagggcaat ccgcagccgt tcttctggcc
     7261 cggcctctgt ctgccgacac tcctccagaa catcctgcca ctgccggcga aggtgtggac
     7321 gttcattcag ttcagtaaaa ctgtatttat ccgcaagata gtcaattcgt gtacgcatac
     7381 tgaaaggaat tccgtataaa gattcagcag acaaaacttt atcagccagt aaaaattaac
     7441 ggaagcattg atattcctcc caacaatcac cggatgattg ttgcaataac ctgcgacata
     7501 aaggactgaa cagccgtttt taacgccacg tattgctctg atgaaccagg ccgggcaacc
     7561 cgaaaacatc cttccacccg gcattgtccg cctgcgctta tcacctgcca ggtctgttgc
     7621 agtaaatcca ccagttgcgc acattcttca tcagccatga cgccgactcg cggatggcaa
     7681 atactcgtct ggctgagcag caacagacat cgtatctgtg ctccttcatg ttgttcccgc
     7741 aaccagcgta ataccaccgg cgctgatgga ctgaccgtgt gattatgctc cgtaatacgt
     7801 tcgtgtaccc gtcggtgaaa ggaactacag aatgtctgaa tctgttgacc atagattcct
     7861 tctgtcgaat ggagcgtgaa gtggattgcc agcagacgag gccagtgatc caccgcctac
     7921 tgaacaaaac gccggatttc ccccggctct gaaattaagg cttcggttat ttgcactatt
     7981 ttatctctgt ttaatttggt taagccggtg aagacgcatc aacaaaagca cgcttcgata
     8041 caaacagctg tgactggcga tacgaaagaa atgacgaatc agtcaggatg acaaactgct
     8101 ggctgaccgg aggggacaca ggaagattca cgggggggcc agcaccaggg acaggggccg
     8161 acgccacaat accagcgctg acatctggca cattgccagc gtaccggtat cacaacacgt
     8221 ttcatacttc tgccctccat gattcgttgc tgcatccact gctccacttc acttttgtac
     8281 ttgcggctgc tgcgccacgg tttgatgggt ttagagaaat ggtcttcact gaatcagcat
     8341 gtaaaaccct ttgtcgggcc ggtgcgtcag caccaatgat ataagacagt aaaatgagca
     8401 cgctgtcgtg atctgtgatt gaggtcaaca tgctcagaat gtcccctgtg agattgatgc
     8461 gatacaggag aatgtcggcg tgatttcagc gtcgtcctgc tggtgtgata tcgcaaatat
     8521 gccaatgaga taacgggatt cctgattgcg tacaccacga cgagcgcagg aaaaatcact
     8581 gaacgctctt tgaatctatc ttcgctgtca ggccaccaat gaaccccgtg ccacattccg
     8641 ggggcttctg gtgctgcgcg cttcgtccag tgacacaaag cgcttacatt ggctatgaca
     8701 tccatcatca actgatccag aacaggggca tcgaaatatg cctctctgac cgttatattc
     8761 cggcgtatcg ggagaaggtc agaagtaccg gtcatcagat cgggcaggat attaatcaat
     8821 gcagcccccg ggtttcatcc tcctgaggca ggcaaaggct gagacgacgc ccccgtgtgg
     8881 ttgattgaca ccaccgtgcg acttcgtccc accactcaca atcaatctcc cgggttttat
     8941 tcaggtaagc ctgcagggcg gtggacatcc ggtgtcgcac cgtggtgtca cgaacggggt
     9001 tggtgcgaaa caccggataa aatgcaggtt gcgatacctc tgaacgccct gcgcagagcg
     9061 gaaattttgg attaagtact cgcacctccg cagtccagaa acaagtcggg ctggtagctg
     9121 taaacagact tcgtacatac cgctctgcaa taaatccccg taccacaggc ctcacagaac
     9181 ttcttcccat gaaaaaactg ctcacacatc acacaatgcc actccagcac gaccggtaat
     9241 ggcgataaaa acatcgccat atcctcaatg taagggtggg acttttccgg attcagcacc
     9301 acgcagtccg tcttctgttg cgcggtcagg gcatgtaaat cgtgctcaaa ccacgccccc
     9361 tgagcacctg tctgcaaaat caaccggcca cgacaggaaa ggcagaaaca atgccggata
     9421 tttctgctga gcgcagagtc ggcactgata atgtgctcac ccggcgtgat ccccagcccc
     9481 gtttttatac cgttcattct gctaccccct cctcactgaa acgcccggtc tggcagtgtg
     9541 tgcagtaccg ctccccataa taatcgtggt gacattgtct gcagtaccag ctggctttac
     9601 gtaccacggg taaggcatcc ggtacgaatt gctgcagacg tttaatcaac cgtatttctc
     9661 tgcgttccgg cctgacataa gggcactcat gaccgtgctc cgtcagccct tcgtcagcgt
     9721 gttcaaacca gggccgttca gtgtcgtatt gcgggtggta tctgagtgca ctgccgcaaa
     9781 ggtggcaggt gtagctatcg taaggtgcag tctgtgcggt acgggcaccg gtcagacgtc
     9841 cgttgccatc aagtgcgaga aaagattttg cgtacatagt atatgttcct cagccgccag
     9901 acgacacgca ggcaacagcc gtcccggtac gggcagcgtg gtcagggtgt gaatggcggt
     9961 tcagttaaaa tctggactgg agtttgggta aagcaggctg gttgctgtta cagcactgtg
    10021 gttgtcacac aatggcccac tgacgtaaaa aacagtcagt aagcagagat ggagattggg
    10081 ggatgtgctg tatcggttgc cggaaggaat ggtaaagaat gtgtcatggt taaatctccg
    10141 tatggttcat acggaatgac cacgccgtaa aaaatgcgca gtgctgctga agtggccgcc
    10201 ggaaccgaca gcaaagatta gcgccagccc gtacttgtcg ttcaggggtt tacaccagag
    10261 cacatatccc cggcaggggg ctgtccagtc gtggtcgacc ccggttactt ttttcaggaa
    10321 gcccgctgca tttcaggatg atgccacatt ctttctgttc ccgtttggcc aggtagctga
    10381 tggcatagcg taaatcattc accgctttgt gatctgaata gtgaatgacg tggttaatgt
    10441 ttaccggata atttttatta aaccggcacc agtggtagta accattaccc tcggtaaccc
    10501 gcctccagat atctcccatc aatctggata ccagatacga actcctgtgg ctctggccgt
    10561 tcagatagcc cacaaaatga atgtgaagcc ctttacgttc tgtatattcc atcacccagg
    10621 catacccgac cagtttctct cccacctcgt taatcagttg cagcatctcc gcgcacatgg
    10681 cgtacctgca ggcacatctg tagctgaggg tgttttttcg gtaagcaaaa tccattctga
    10741 acggtaacag tttcgaatag accccgaata atgaatccag atgctggtta aagtgctcca
    10801 ggagctgggg agccatttcg taagcgggat tggtgttata agtttgcatg tttcttcctc
    10861 gtgttctgta acacaggaag aagcgttgcc gtaatttttg aagaagatgt tctcaggtag
    10921 tagggtgaac gtgtttcctc cggtaacgac ttccacgggt atcagaacca ggcagagaac
    10981 agcatacgta acagccatac agcgcatata acgcctgaag ctctggtaac cagactgcag
    11041 cggaaaggcc tttataacgc gtgtgggcaa tcactttttc cagtgaacaa cagcggaact
    11101 tacgcaggtt ttaacttcca ttatcgttat tttgtactgt gattaacgtc agaatggcct
    11161 gacattatcc cgaaagtctt tccacttgct gtttctccgg taataaaaca ttttaaatgc
    11221 tgaaggtata aagccataac cagatattgt aattaacgta gcattacaca tatcaacgta
    11281 ccagataagt aacagaggta caggattgaa caacacgccg gaagactgtt taatgacatg
    11341 aggcatactc ttttacgagt aagattatgt attaccacta atactcatca ggtgagttgc
    11401 aggagcagat tattaacaga aatccttcaa acacagttat ccaggatatt aacacgggtg
    11461 aagccatact cttggctata aagatgatga cgggatcaga atagttatta atccatacaa
    11521 caaaatacag aaaacaacag aaaaaaaata acagacatta cttaacaaag aacattacag
    11581 tcagtgtgta cattacagat ttacattcag gttttacggg actacattac agcagagagg
    11641 ggtaagaaca gagttacaag tactactaca gagaatatca ctgcacagat acttctatga
    11701 gtatatcttt atgagagatc cgtctacagg attgggttat aaggtgtatt tacaaggtat
    11761 aattacagag ttcatttgca tgaccatact tctataaata atactaatta accttaatta
    11821 ttttttaatg gtacatgaac tacctgtagc tgtaacgata aacaaatcat ccaaaacgaa
    11881 aaatactggt tatcacagtt cagagaactc gtatgagtgt ccccctgagc aatgcttcat
    11941 tttctgtggt tctgtgtgat agagttatac tgagtacagt ggcagtaaca ctgctttcca
    12001 tcacttgagg ataaaattca ttaaaaaatt aattgtatga attaacaggt ttttttatga
    12061 aatgttatgc atctcaacca gtatgattaa cagaaaactt catcatgaca acacgtataa
    12121 cgttcagtat gaccagtgat gagacgctgc gaattgtgga tgagtactgt catactcaca
    12181 aactttcgcg ctctaaggta atcaacgcat tactcagcgc cacagcacct gtccttaatg
    12241 atattaactg ttattatcaa cttgcaggcg aactgcagtc acgcttatta aatggagtgt
    12301 atcaacggga tcttcctcgc aaacgcaatg tcgtcagtgc agagaagtat tgtctggaaa
    12361 tctgggaaaa caaacttttc accagaagaa tcctggagtt tgacagttca aatggtgttc
    12421 tgtacgcgct gaaacataaa agacactatc gccgggataa gatgattgga agggttgaaa
    12481 gtcggtgtat caaagacatt tgcgaatacc agatgcagct atccggggag aagacgaaat
    12541 atgcctgttt tatttatatc gaaagaacaa tatacaatca cgataatcca tctgataaaa
    12601 tacctgtaaa agcggctgtt ggtaatgccg tcatcctgct ggcaaaggat gtgatatata
    12661 acgagtactt ttttgatttg cggcagtcat tttttgtgag cgttacagat ctgatggtct
    12721 ctggcgcaaa aggtattccg gagacacaga aatatccgga cgtttattgc tgggtcccgc
    12781 ttttctccat aaattccggc gtgctcatta cccctgtata taaaattgac cctctgaaac
    12841 ccgtaacggt aaaaaaaccg gataagatca ctgttgtttg taattacaga gaataatttc
    12901 agccttctac cttcaccgcg acagtgttta cctctgcttt atcttccgtt cagtaaatac
    12961 aaaccaccgc tctggcaggt acagggcgtg gtttatctcc gatactcccc tgtgcagtaa
    13021 taatcatcac tgaaaatact gattccgtca cggcaaaacg ccatcccata ttccggcaac
    13081 acaaaatcat cttccgttta taccggcgtc actgacaaaa cagacaacct gtattgcctg
    13141 tacacaacag tttctctcct ggcaggaata ttcccctact cattttttac gtttctcccc
    13201 ggttgttatg ccggtgaagg tggtgcgtcg ttttcatcac caacgccggt tgagattaac
    13261 aacatccgga ggaacattct catgaatacc ccagtttcac tgatggatga ccagatggtc
    13321 gacatgtcgt ttatcactca gctgaccggc ctgaccgata agtggtttta taaactcatc
    13381 aaggatggag cctttccgcc ccccatcaaa ctgggccgta gctcccgctg gctgaaaagc
    13441 gaagtggaag cctggctgca ggcgcgtatt gcacagtccc gtccgtaatt cctgaccgtt
    13501 atccgttcca ccgcagcaaa ggcatgatcc ggcccccgga ggcattttgc tgcgcgctaa
    13561 aggagagaat attatgcaaa aacttgaaaa ccggtgtgag ctgctgctca tccagcatca
    13621 gaaatggatg acaagcgtga cgaggcttat cgtcgctcat ggaatggggt ctccgcatct
    13681 tcatggttat caccgtctga ccctggcaca cttttttctg ccggaaaaag gctccgtcat
    13741 ttcggtggcc cc
//
LOCUS       DAZGSP010000131        13497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZGSP010000131 DAZGSP010000000
VERSION     DAZGSP010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.132"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            118..948
                     /gene="yjiC"
                     /locus_tag="ACHK74_004397"
     CDS             118..948
                     /gene="yjiC"
                     /locus_tag="ACHK74_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFP0223102.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDNELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1089..1862)
                     /gene="uxuR"
                     /locus_tag="ACHK74_004398"
     CDS             complement(1089..1862)
                     /gene="uxuR"
                     /locus_tag="ACHK74_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFP0223103.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2077..3537)
                     /gene="uxuB"
                     /locus_tag="ACHK74_004399"
     CDS             complement(2077..3537)
                     /gene="uxuB"
                     /locus_tag="ACHK74_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFP0223104.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFSLMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3618..4802)
                     /gene="uxuA"
                     /locus_tag="ACHK74_004400"
     CDS             complement(3618..4802)
                     /gene="uxuA"
                     /locus_tag="ACHK74_004400"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP0223105.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5142..6485
                     /gene="gntP"
                     /locus_tag="ACHK74_004401"
     CDS             5142..6485
                     /gene="gntP"
                     /locus_tag="ACHK74_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFP0223106.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6728..7630)
                     /gene="fimH"
                     /locus_tag="ACHK74_004402"
     CDS             complement(6728..7630)
                     /gene="fimH"
                     /locus_tag="ACHK74_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFP0223107.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YVRTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7650..8093)
                     /locus_tag="ACHK74_004403"
     CDS             complement(7650..8093)
                     /locus_tag="ACHK74_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0223108.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(8166..8696)
                     /gene="fimF"
                     /locus_tag="ACHK74_004404"
     CDS             complement(8166..8696)
                     /gene="fimF"
                     /locus_tag="ACHK74_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFP0223109.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8707..11343)
                     /gene="fimD"
                     /locus_tag="ACHK74_004405"
     CDS             complement(8707..11343)
                     /gene="fimD"
                     /locus_tag="ACHK74_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFP0223110.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHHLAGFFVRLFVACAFVAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNMASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIITVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCI
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11410..12135)
                     /gene="fimC"
                     /locus_tag="ACHK74_004406"
     CDS             complement(11410..12135)
                     /gene="fimC"
                     /locus_tag="ACHK74_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFP0223111.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKRENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12172..12711)
                     /gene="fimI"
                     /locus_tag="ACHK74_004407"
     CDS             complement(12172..12711)
                     /gene="fimI"
                     /locus_tag="ACHK74_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0223112.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12776..13324)
                     /gene="fimA"
                     /locus_tag="ACHK74_004408"
     CDS             complement(12776..13324)
                     /gene="fimA"
                     /locus_tag="ACHK74_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP0223113.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATHTDVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         3281 a         3489 c         3261 g         3466 t
ORIGIN      
        1 agagttgata tcactacaac agaaacatgc atgtcatttg tttacttctc tttttactac
       61 agaaaaattt atctattttt ctaaacatca gtgaaatact cctaaaaggt aactattatg
      121 tctatgccat taagcaatgc ccttcagagt caaattatta cagataatca cttccttcat
      181 cacccaaaag tcgacaatga gttaacacgt aaatatgaac gagctcgcct ggatacggaa
      241 aatatctatc ttctgcctct tgctcgtggc aataaccata attacgatgg aaaaagcgtt
      301 gtcgaaatta gaaaacttga tattagtaaa gagtcctggc catttaatta tgtgacagaa
      361 gcatgcagag aatccgatgg tattacaaca acaggacgta tgctctatcg aaacttgaaa
      421 atcacctcag ctttagacga aatatatggt ggtatatgta aaaaggcgca tgctgctaca
      481 gaattagcag aaggtttacg gctaaattta tttatgaaga gccctttcga tcctgttgag
      541 gactatacag ttcatgaaat aacattaggc cctggttgca atgttccagg ttacgccgga
      601 accacgatag gatatatttc aacccttcca gtttctcagg caaaaagatg gaccaatgag
      661 cagccaagaa ttgatatcta tattgatcag ataataactg tctcaggggt agcaaattct
      721 tccggattcg cccttgccgc attacttaat gctaatattg gaatgggaaa tgacccaata
      781 attggtatag aagcctatcc cggtaccgcg gagatccatg caaaaatggg atataacgtt
      841 atccccggtg atgaagatgc gccactcaaa cgcatgactc tgcaaccgtc atctcttcca
      901 gaactgtttg aattaaaaaa tggtgaatgg aattatattg gtaaatagcc actgagaaga
      961 atgagtacta atattcaggt tatacagata tttaaacaat accctgcaat ttacaaagta
     1021 atactcattg cgacttctta atttaagaaa gcgcaaggaa cgtttaccct tgcgcttatt
     1081 ataataagtc aggcgtcgac tttatccagc ggccatgaat caaacagata gccgtcaaaa
     1141 taaatatcgt caacgttcga gaattccagc agacgttgct taacgttttc cagatgctgc
     1201 cacattgcca gcttcgccgc tcgggcatct tttttgatta acgcggcgag gatctgtttg
     1261 tgatcgccca accactcttt gcgatacagg ctgtcatcca gatggctgtg caactggatc
     1321 cacattgggt tgttttcccg ccactgccag gactgacgga acagctccac cagcatgctg
     1381 ttatgcgttg cttcggcaat agcgagatgg aactgcatgt caccgctttc gctgctgccc
     1441 ggcgcactgg aagccagttc acgctcttcc agttgcaatg cctgacgcat tttgacgata
     1501 tcttcgcggg tagcctgcag ggcggcaaac tcggcgatgt tactttccag taactgacgc
     1561 gcctgtaaca gctcaaaagg acccgcatca ttgcagacgt tggcatccgg actgtctgtg
     1621 ttgtggctgc ctgagctatc aagaacatag ataccggcac cccggcgcac ttccaccagc
     1681 cctttgatct ccagcatgat cagcgcttca cgtaccaccg tccgcgtgac atcaagcatt
     1741 tctgcaattt cacgctccgg cggcagccgt tcgccagggt tgtacggcgt ctttatgatc
     1801 agatcgcgga tcatcgcccc gacttcctgg taaggtcttt gcgcagaggt ggcagatttc
     1861 atggtgttgt gtccgttagg tgcggttaat caaatttttg ccggatgcgg cgtgaacgcc
     1921 ttatccggcc tacaacgggc aattttacgg cccccgttaa tctcaaacta taccattagc
     1981 actggaagtt ggcacgtaca ggtgggcaaa aaaacgaaaa ctggtcaacc aaatatcagt
     2041 ttgattgacc agtaagtctg tagggtaatc agttagttac agcgcagcca cacactcgcg
     2101 cgcaccgcgt tcgcacagct gctgatatgc cgccgtcact gcgccaacaa agtcggcatt
     2161 ctgcggcaga tcatcggcaa aaataccgct caggcccagc agcgctttca cgcggtctgc
     2221 gccctgatac tgcgcgttga tcttctggaa ctccgccagc atcgggtcga ccacgtcaat
     2281 ggcattaccc tgctcatcca cgccctgggt gtaacgcatc cagccagcca cgcccagcgc
     2341 caggtgacgc cagctgccgc cgttttgcag gtgcagacgc accgggtcca gcagacgctg
     2401 cggtaacttc tggctgccgt ccatcgcaat ctgccaggta cggtgacgca gagacgggtt
     2461 gctgaaacgc tcgatcagca acgtcgcata ggcgttcagg tccgtacctt ccggcatcga
     2521 cagcgttggc gcttgttcct gcatcatcag ggagaaggcc gctttgcgat aagccgggtt
     2581 agtcatggtg tcggcaatgg tttcatagcc gccgaggtaa ccgaggtacg ccagaaaaga
     2641 gtggctgccg ttcagcatac gcagcttcat catttcgaac ggcacaacgt ctgcaacgaa
     2701 ctgtgcgccc actttatccc agtccgggcg accattaacg aagttatctt caatcaccca
     2761 ctgacggaac ggttcgcagg caatggcgca cgggtcgtaa acacccagct ggtcagcaat
     2821 ttcctgtaag gtttctggcg tcgccgctgg aacgatgcgg tcaaccatgg tgcatgggaa
     2881 ggtgacgttc tcttcaatcc atgccgccag ctgcggatcg cgtgcctgag ccagtcccag
     2941 taccgcgacc ttcgccacat gaccgttttc acgcacgtta tcgcaggaca tcaccgtaaa
     3001 cgctttcaac cctttttcac gacgcagacg caaggcttcg acgatgtaac caatcgcgga
     3061 cttcggcgca gtcgggtttt ccagatcgtg cttgatcagt gggttattga gatccagctg
     3121 accgcttgcc gcatcagcgc agtagccttt ttccgtgacc gtcagggaga caatcgccgt
     3181 ctgcggacgc gccatcgcgt tgagaatacc ttcgcaaccg tcgatttccg gatgcagcgc
     3241 ttctttcatc gaaccgataa ttttcagctc ggtgctctct gcgccttttt ccgctacggt
     3301 gtacagcagt tgctgttttt tcaggttttc gatcagcacg cggtcgttgc ctggcatcag
     3361 gttaacttcg cagatgcccc agtcgctgtc ggtgctttcc agcagatgat gggtatacag
     3421 cgcctggtgc gcgcggtgaa acgccccgca accgagatgc acaatgcgtg attccagacg
     3481 agaatgatcc catgacgggc gggcaaccgg cagattgctg tcaacaatag tagtcatcgt
     3541 aaactccaga atccgtcttc aaagagtgac ggcaaggaag aaggaaccgg cacggcgcgc
     3601 agccatgccg gtggatatta acggctaaag aaagcgcgct ggatcgccag ttcgacaccg
     3661 cgaacttcgg ccaggccttt cagacgacca attgcggagt aacctgggtt ggttttcttc
     3721 ttcaggtcgt ccagcatctg atgaccgtgg tccggacgca tcgggatcag gtcttctttg
     3781 ccttccgctt tacgacggtg ttcttcttca acaatcgctt tcaccacttc gtacatatca
     3841 acgtcaccgt tcaggtgcgc cgcttcgtgg aaggttttcg ggttatcttc acgcatggtg
     3901 gagcgcagat gggtgaagta aatacgcgga ccgaactgct tgatcatatc aaccagatcg
     3961 ttgtcagcac gcacgccgta ggaaccggtg cacatggtaa aaccgtttgc catgctgttt
     4021 acggtatcaa ccatccactg catatcttca atggtggaaa caatgcgcgg caggccgagg
     4081 atcgggcgcg gcggatcgtc cgggtgaaca gccatacgca cgccaacttc ttcggcaacc
     4141 ggaataatcg ctttcaggaa gacggcaaag ttttcgcgca gtttggcttt gtcgatatct
     4201 ttgtacagct ccaggtgctt acggaactgg tcgagggtgt acccttcttc cgcgcccggc
     4261 agaccagcga tgatgttacg ggtcagacgc gctttgtctt cgtcgctcat agtggcgaag
     4321 cgttcagctg cctgagcaat ttcttcttcg gtgtaatccg cttccgcgcc tggacgtttc
     4381 aggatatgca tttcgaatgc agcgaattcg atctggtcga agcgcagagc tttggagccg
     4441 tctggcagca cgtattcgag gtcagtacgg gtccagtcga gcaccggcat gaagttgtag
     4501 cacacggtgc gaatgccaca ctgcgccagg ttgcgcagag tctgctgata gttagcgatc
     4561 cactgctcat agttgccagt gtgggttttg atatcttcgt gaattggtac gctttctacg
     4621 acagaccaca ccaggcctgc gtcttcaacg attgccttgc gtttgaggat ctcttctacg
     4681 gaccatactt cgccgttcgg gatatggtgc agcgcggtaa ccacgccagt tgcgcccgcc
     4741 tgacggacat cagctaaaga aaccggatcg tttgggccgt accagcgcca ggtctgttcc
     4801 atgatttttc ctctttcaca tagtgggttc cacagcaaag agacgcggaa ccctggagtt
     4861 gtttaggtat ccggatgaac aaccagaccg cagccgtggc gttgcatcgg atgagatggc
     4921 gtataagttc taccttttgg taagacatgt caacccgaat tctgaaattg gttaaccaca
     4981 tcacaagaat tttactctcc tggttgttgc tgcaaattgc agcaaaaggt tatcgatggt
     5041 tattgatcag aggatgtgac attcatcgca acaatggttg accaatttac ataacatgtc
     5101 gcccaaaata acaacggttc aaccaggtaa ggtaaaacgt tatgcatgtg cttaacattc
     5161 tctgggtagt attcggcatt ggcctgatgc tggtactgaa tttgaagttc aaaatcaatt
     5221 caatggtggc tttgttggtg gcggcgctgt ccgtcgggat gctggcgggc atggatttga
     5281 tgtcgctgct gcacactatg aaagcgggct tcggcaacac gctgggggaa ctggctatca
     5341 tcgtggtgtt cggtgcggtc atcggtaaat tgatggtcga ctccggcgcg gctcaccaga
     5401 tagcgcatac gctgctggcg cgtctcggtc tgcgctatgt acagctgtcg gtgattatca
     5461 tcggcttgat ttttggtctg gcgatgttct atgaagtggc ctttatcatg ttagcgccgc
     5521 tggttattgt tattgccgcc gaagctaaaa ttccgttcct gaaactggcg atcccggcag
     5581 tagcagctgc cactaccgca cattcactgt tcccaccgca gccgggtccg gtggcgctgg
     5641 tgaatgctta tggcgcggat atggggatgg tttatatcta tggcgtactg gtgacgatcc
     5701 caagtgtaat ctgcgcaggt ctgatcctgc cgaagttcct cggcaatctt gagcgcccaa
     5761 cgccatcatt cctgaaagca gatcaaccgg tagatatgaa taatctgccc tctttcggcg
     5821 tttcgattct ggtgccgctg atcccggcga tcattatgat ctctaccacc atcgccaata
     5881 tctggctggt aaaagatacc cctgcctggg aagtggttaa ctttatcggt tcctcgccga
     5941 ttgcaatgtt tattgcgatg gtggttgcat tcgtactctt tggcaccgca cgtggtcatg
     6001 acatgcagtg ggtgatgaac gcttttgaaa gcgcggtgaa gagtattgca atggtgattc
     6061 tgatcatcgg tgcgggtggc gtgctgaagc agaccatcat cgacaccggc attggcgaca
     6121 ccatcggcat gttgatgtcc cacggcaata tctcgcccta catcatggca tggctgatca
     6181 ctgtgctaat tcgtctggcg acgggtcagg gtgtcgtttc ggcgatgacc gccgccggga
     6241 ttataagtgc tgcaatccta gatccagcaa ctggtcagct ggttggcgtg aatccggcgc
     6301 tgctggtact ggcgacggct gcgggttcca acaccctcac ccacattaac gatgcatctt
     6361 tctggttgtt caaaggttac tttgacctgt cagtaaaaga cacgttgaaa acctggggtc
     6421 tgctggagct ggtcaactcc gtggttgggc tgattattgt gctgattatt agcatggtag
     6481 cgtaaaagga caccagagcc tgccaatggc aggctctgac tgatgacaaa gtctaaaaat
     6541 gtgcccagac agtcctctcg cccctctggg gagagggtta gggtgagggg aaaagaccag
     6601 caaagtgcgc tttgtttatg ccagatgcga cgctaacgcg tcttatctgg cctacaaagg
     6661 gctaacgtgc aggtttttag cttcaggtaa tattgcgtac ctgcattagc aatgccctgt
     6721 gatttcttta ttgataaaca aaagtcacgc caataatcga ttgcacattc cctgcagtca
     6781 cctgccctcc ggtacgtacg taatttgccg ttaatcccag acttaccgcc gaagtcccta
     6841 ctgctcctaa cgataccgtg ttattcgctg gaataatcgt accgttgcgc gtcaactgta
     6901 cgccgacgcc ctgcgctggt gaaaacgacg cggtattggt gaaaatcgag ttgcccgcat
     6961 ctgcggttgt gccggagagg taatacccca ggttttggct tttcgcacaa taaacggtaa
     7021 gaggaattgg cactgaacca gggtagtccg gcagagtaac ggtgacatca cgagcagaaa
     7081 catcgcagcc gccagtaggc accaccacat cattattggc gtaaatattc cacacaaact
     7141 ggaaatcatc gctgttatag ttgttggtct gtcgcaaaat aagcacggca attaatgagc
     7201 cagctttaat cgccaccccg cccgcactgc tcacaggcgt caaataaagc gccaccggcc
     7261 acggcttatc cgttctcgaa ttataaacaa cccgcggcgt ttcgctggtg gtcggaaatg
     7321 gatagctact gccactatat tttacggtcc cggaaaaatt agataacacg ccgccataag
     7381 ccgagcctcg ttgcagtgtg acatagtctg taatggtttc cggataatcg ttatggcaaa
     7441 agatttgcgt cgaaagatct acgaccaggt tttgccccac attcacggca ggcgcaaggt
     7501 ttacataaac attagcgctg ccaccgccaa tagggatagc tgtaccattg gcggttttac
     7561 aggcgaatga ccaggcattt accgaccagc ccatcagcag tacagcaaac agggtaataa
     7621 ctcgtttcat tacaatcatc tcttcgggtt cagctgtagg tataggtgat gctaatcact
     7681 gcctgaatgg ttccctgagt ggctccgcca tttactgtca atgctctgac ctgtaacggg
     7741 aagtgcgctg attgtgagga atcatccacc tgaactgttt tggttgcgcc agtattcaat
     7801 gtgttgccac tgtcatcctg tagctctaac tggatgtttt gcgcggtccc ctggttttta
     7861 taatatccgg tactgtcggc tgccccgctg aagctggcag tgaccctcga cgttcccacc
     7921 ggacaattag tcaactcaag cgcaacatca tgccaggccg atgccgcccc ggcagacatc
     7981 agactgaaag aataaagatc gccgagatca accgtggcat tggtggtgga aactgtgcac
     8041 ggtttggcga cgaccttacc gttcaccgtg atggtgacat cggctgcctg tatcgtcgca
     8101 cttgcgagcg ccaatattgc cgccaataca tacccacgtt tgcaccattt catgagcatc
     8161 tccagttact gatattcaag agtgaaggta gccgtggcat tgatatgccc cgcagtgaca
     8221 ggcacctgtg tcgccattag ccgggcgtaa aaattcaacg tatttggttt acccggcgtc
     8281 agggtcgtcc acgaaatcgc ggacgatgga gcattaaggg gtatctgatt ttgctgctca
     8341 ttcagaagct gtattcccag tcccgaagcc gctgataccg tattttcaag tgcaagcagg
     8401 ttggcattgt ggctatctgc aacgccggta aacccaacct ttacggcaga aacggcgtta
     8461 ccacagggtg acagcaaaat acgaaatgga acgacaggag tcgttgcgcc aatgttgtta
     8521 aattgcttcg ccgcgttttc catcagatca acagtaaaat tggttgattc agcggccaca
     8581 ctacagccgt tatctctgac atagccgcgg atagtaatcg tgctatccgc agccaaaacg
     8641 cgacttaccg ccagccacaa aaaagcgcac agaagataaa aaggtttgtt tctcatcacg
     8701 ccccccttaa cgacattcag ctgatagctg ggttaataac tgctgctgac tctctggtgg
     8761 cagttgataa ttggcgatac agtgagcatt ttcctcttct ccccatttca cctgaacttt
     8821 tcccgctaaa ggcattccgc tgaggtaaac ctgaccatta tccgcaacaa tgccgctact
     8881 ctggctactc tctgatgtca ccatcgcccc aaacggcagc ggcttattat tgtgggttag
     8941 cgtcatgagc agttttatcc caacgcgcgc tttaaactct gctcgcacga tcgccccacg
     9001 agtgggaaca acgttagcga ccgcgttatc taaatcgacg ttatcagcca gggtattggt
     9061 atccagcgcc actctatttt cccgatattc agtggcataa ggcagcacgg cataaccacg
     9121 ccagtcggta cgcacccccg tctggttttc gacttttgca tcttttgcgc caggcgcttt
     9181 aacaagcacc accgtatcgt ttaacggctg ccccagcgtt acgccattgg catgagccag
     9241 taccccaccg ctgactccgt aatagagctg cttaatatca tcactatggc tgtaaccgat
     9301 attggcattg ccgtaaccac cgcgataatt cagcgtggcg tagcctgtgc ttccgctatt
     9361 accatcgcct cccccggcat agccggtttg cacgctatag ctgaggttgt tgtcttccag
     9421 caacgtaccg tatacaccag ccagattggt cattcgaccg ttgagatcgt gtgacatgct
     9481 gtagctggca ctggcatgtc gccactgaga tttactgtca gaacgcagcc agtggctgaa
     9541 aggaatattg acgttaagcg ctaacatctg atcacgtcct ttttgccagg cgtttttcgt
     9601 caggctatag ctgagcgtcc agttgatatc ttcgaacgca gtatttaatc cagcctggaa
     9661 ttgctcatcg acattactcg ttccccaata agtttgatgg ctaccactca aatacagtgt
     9721 tgatgagcgc ccgagttgct gagtaacggt gagttgtaat ttcccgcgtt tgttataagc
     9781 gaggttgtaa tagtcggtga atttcggctt aacctgaata actccgtcct gtgtttcgat
     9841 gttgtagcca ttcattcgac tgtatgttgt atcagcgaaa ttaaaatatc cgctggtcga
     9901 ataacggtaa cccactaact gaatattcgt gcctgactca ttgagcgatt tgttatagag
     9961 aaaacgcacc gattgtccgt catgctgact gtcatcggga agtgtggaat tagcctgcgt
    10021 catatccaca gacagagcgc ccagtgcccc catgtttttc ccgataccga aattaaaagc
    10081 acgataacga tccgccagtt gcgttccacc atatattgtc cagccagccg gaaggccgtg
    10141 gagtaatgta ctctggaaaa agcggggttt ttcctgctgc gcatttccac tacggtattc
    10201 tcctgccgta atggaataac gagtatgccc ttcacgttgc aaaagcggga ctgacgaata
    10261 gggtacggta ataatctgcg tgctgccgtc agcctctttg atcgttacct gcaagtcacc
    10321 actattacct gcggcataga tatcgttgat ggtaaaaggc cccggtggca ccgtactatt
    10381 ataaatgtca tacccatttt gtttaatagt gacctgtgca gtaccacgag caataccgtg
    10441 gatcaccggg gcaaatcctc tttggctatc gggtaacata ttgtcatctg aggccaattg
    10501 tgcgccgcga aagttaatac catcgaaaat atcgccctga gtataaccat cacccagcgt
    10561 cagccgggaa cgtaacggta ttatgtctcg ctcaagccag gtattgatat gctgccattt
    10621 atttttgcta cctgatgatc tgtcgctact gttataactc caggtggtat tgtcgcgtaa
    10681 acgccacgca ccaatattta acccactctg taggtttaaa tatgcataat ggctgttacc
    10741 cccaatccga ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat
    10801 accgggatcc cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg
    10861 agggatcgtc aggttgagtc gctgctgacc aacatctaaa tgcgcagtag cgtcctggac
    10921 cattgtggtt aatggcacac aggcatcatc cgcgaggaga ttcataccgg cgacagaagc
    10981 catattcagc cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc
    11041 actgtcgccc gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat
    11101 atcgacgcga tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc
    11161 cacagcctgg ggatcatccg ctaaaaagcg cgggttaaaa tagagttcgg cagatgacaa
    11221 aggtgcctgt gcgacaaaag cacaggcaac aaagagccgg acaaaaaaac cagccaaatg
    11281 atgcttacga atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat tcagatatga
    11341 cattattcct ccggtttaaa atcacccggc agtcaattct ttttattcag gcgaaaaatt
    11401 ccccctgcgt tattccatta cgcccgtcat tttgggggta agtgcgccat aatcatttat
    11461 tgttcggtaa gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc tttcgcccat
    11521 tggaggcacc aatgcatttt caagaacccg ggttccggca ttcaactctg ttaccgtcag
    11581 gtaatagggt gtcgggttaa tcagcgtcag ggaattcgcg ctacgacgaa atcttaattt
    11641 ttctgcggcc tgatcgggtg gcaacgctaa tttagccgga cgatagtaca gtttaatgcg
    11701 gctgataatt gcgagctgta gcgtattctc agtcaatttt gatttatcca ttgacggaat
    11761 cgctttaaca ttcatccaga ataaactttc ccgatcctgt ggcaattggt tattagttgc
    11821 atcaagaata cgcaaggtat tctctctttt tcccttcatc gcaaacagag gaggcgtcac
    11881 gataaaacga ccatccttta caccatcggc attttccacc catgattgaa ttaaataggt
    11941 actattttca tcattatttg tcacggcaag ttgcacttgt ttttgccctg ccggataaat
    12001 tacgcgagtc gcacctaagg ccactcccgc ttcagcgcgt ccggcaacca tcattgccat
    12061 gaacatcagg atacctgcca gcaagcagaa tgttatttcc tgcgatttcc ttacattgac
    12121 gtttttatta ctcactgtcc tgttcctgtt atcacattat ctgctgaaca attactgata
    12181 ggttaaagag aaccaggcct gggcattggc gatgccgcca gtaacccgac gcccggtagc
    12241 acgatatttg gcgatgaaat gtagcgaagt agagcctgag taaagccgtt tccagtttgc
    12301 tggaggacga ttaatcggta cgaggtttcc ttcatcatca aacaacgcta cgccaatatt
    12361 ggtggctatc cctggcccct ctcccacgga aagcacatcc ggatttttac catccgcgac
    12421 accgtgaaac gccacaccca cacgttcact caccaccgtg ctacattccc gtaaatgaat
    12481 aacaaaaggc accggtgcgc tatcttcccc taccgcatga aaccggttac tgctgatttg
    12541 ccccatattg accgtcattt gtttatcacc ggcttcaatc cggcaagttt ccgcaataat
    12601 gacgccctga aattgcatat ttccgccggg cagcgtggta ttccatttat ttccggccag
    12661 agcaaacatt ggcagcaacg acgctaatag aaataatctc ttccttttca tcgttatttt
    12721 tatcgcacaa gggtgggcat ccctgcccgt aatgacgtcc ctgaacctgg gtaggttatt
    12781 gatactgaac cttgaaggtc gcatccgcat tagcagcacc cggggttgcg gcaccggttg
    12841 caaaataacg cgcctggaac ggaatggtgt tggttccgtt attcagggtt gtttctgaac
    12901 taaatgtcgc accgtccagc gccagcgcag cacccgttct gtccaggatc tgcacaccaa
    12961 cgtttgttgc gctacccgca gctgaactct gcagagccag tacatcagta tgcgtagcat
    13021 caattgccgt acctaagaag gcaacagcgg cttttgtggc aacagtggta tcgcaatcat
    13081 tcagctgaat gttaaaacca acggcagagc tggttgctcc agcctgcttc aggctagcgg
    13141 tacgaacctg gcccaactga acggtttgat caacagagcc tgcatcaact gcgcaagcgg
    13201 cgttaacaac ttccccttta aagtgaacgg tcccaccatt taccgtcgta gtatcggcca
    13261 gagccgctgc ggaactgagg gacagagccg acagaacaac gattgccaga gttttaattt
    13321 tcatgctgct ttcctttaaa aaactatttc taaatcgaca tgggcagtcg ttctgtacac
    13381 tttgttttgt caacgagttt gactgccaac actgcacagt tttcccccaa aagatgaaac
    13441 atttggggcc attttgactc atagaggaaa gcatcgcgca caaacttttt cagttta
//
LOCUS       DAZGSP010000132        13280 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZGSP010000132 DAZGSP010000000
VERSION     DAZGSP010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.133"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..392)
                     /locus_tag="ACHK74_004409"
     CDS             complement(<1..392)
                     /locus_tag="ACHK74_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HFP0223114.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVA"
     gene            complement(605..4828)
                     /gene="rpoC"
                     /locus_tag="ACHK74_004410"
     CDS             complement(605..4828)
                     /gene="rpoC"
                     /locus_tag="ACHK74_004410"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFP0223115.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(4905..8933)
                     /gene="rpoB"
                     /locus_tag="ACHK74_004411"
     CDS             complement(4905..8933)
                     /gene="rpoB"
                     /locus_tag="ACHK74_004411"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP0223116.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            complement(9253..9618)
                     /gene="rplL"
                     /locus_tag="ACHK74_004412"
     CDS             complement(9253..9618)
                     /gene="rplL"
                     /locus_tag="ACHK74_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFP0223117.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            complement(9685..10182)
                     /gene="rplJ"
                     /locus_tag="ACHK74_004413"
     CDS             complement(9685..10182)
                     /gene="rplJ"
                     /locus_tag="ACHK74_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFP0223118.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKDAA"
     gene            complement(10595..11299)
                     /gene="rplA"
                     /locus_tag="ACHK74_004414"
     CDS             complement(10595..11299)
                     /gene="rplA"
                     /locus_tag="ACHK74_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFP0223119.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            complement(11303..11731)
                     /gene="rplK"
                     /locus_tag="ACHK74_004415"
     CDS             complement(11303..11731)
                     /gene="rplK"
                     /locus_tag="ACHK74_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFP0223120.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            complement(11890..12435)
                     /gene="nusG"
                     /locus_tag="ACHK74_004416"
     CDS             complement(11890..12435)
                     /gene="nusG"
                     /locus_tag="ACHK74_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFP0223121.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            complement(12437..12820)
                     /gene="secE"
                     /locus_tag="ACHK74_004417"
     CDS             complement(12437..12820)
                     /gene="secE"
                     /locus_tag="ACHK74_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFP0223122.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            complement(13050..>13280)
                     /gene="tuf"
                     /locus_tag="ACHK74_004418"
     CDS             complement(13050..>13280)
                     /gene="tuf"
                     /locus_tag="ACHK74_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP0223123.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
BASE COUNT         3086 a         3604 c         3270 g         3320 t
ORIGIN      
        1 aacgcaacaa agtaaaatgc cttatctcct ccgtctaagt ccacctcaat cacggcatag
       61 ctaccatacc cggcatgaat aacgttgctt tctggcagcg ccaaaatagt tctttttaag
      121 tgtgtgcgcg ctaagttata acggtgctgg cagaagggac gagattcttt aggcgcgtgg
      181 tacattaacg attgtttttc ttcgttcgtc tgatgttcat aatcttttgt gaagcagtga
      241 agagagtagg taacccaaaa gcgatactct aaaggctctt tgccctcatg atgctgaacg
      301 tagacagcct gatgagcatc aagaaatgat aaatcacgaa tatcaccatc cggatgtcca
      361 aaaggtcgcc acttttccac ttcctgtttc attctcgttc cttaagaaag ttacacagga
      421 atagtatcga atccttgccg agtttgactc aattagttca gtcagtttca ggatattagt
      481 catctctaca ttgattatga gtattcagaa attccttaaa tattctgaca aatgctcttt
      541 ccctaaactc cccccataaa aaaacccgcc gaagcgggtt tttacgttat ttgcggatta
      601 acgattactc gttatcagaa ccgcccagac ctgcgttcag cagttctgcc aggctggcag
      661 atgcgtcttc tgcagtcacc tgcggtgcag ccggagcttc acccgcagca cggcgacgca
      721 tacgatcctg gtggtacgcg taaccggtac ctgccgggat cagacgaccc acgataacgt
      781 tctctttcag gccgcgcagt tcgtcgcgtt tgcccgcaac ggctgcttcg gtcagcacgc
      841 gagtggtctc ctggaacgat gccgcggaga tgaaggactc ggttgccaga gacgctttgg
      901 tgatacccag cagatcgcgg gagtaagttg cacccacttt gccgttcgct tccagttcgc
      961 ggtttgcgat cttgacgcga gagtattcaa cctgttcgcc ttccaggaag tcggagctgc
     1021 ccgcgttaac gatggtagct ttacgcagca tctgacgaac gataacttcg atgtgtttat
     1081 cgttaatctt aacgccctgc agacggtata cgtcctgtac ttcgttaacg atgtaacgag
     1141 tcacagcatg aacaccacgc agacgcagaa tgtcgtgcgg cgcttccgga ccgtcggaaa
     1201 ttacgtcacc acgttctaca cgttcacctt cgaacacgtt gagctgacgc catttcggaa
     1261 tcatctcttc gtacggatcg ctaccgtcta ccggggtgat aaccagacga cgtttacctt
     1321 tggtttcttt accgaaggaa acgataccgc tgatttcagc caggattgcc ggctctttcg
     1381 gacgacgtgc ttcgaacagg tccgcaacgc gcggcagacc accggtgatg tccttggtac
     1441 cgccggattc ctgcggaata cgcgccaggg tgtcaccaga gctgatctgt acgccatctt
     1501 ccagctgaac aatcgcttta cccggcagga agtactgcgc aggcatatcg gtacctggga
     1561 tcagaacgtc gttaccctga gcatcaacga ttttcagtgc cggacgcaga tctttaccac
     1621 ctgctgtacg ttctgcggaa tccagaacca ccagcgaaga cagaccggtc aattcgtctg
     1681 tctgacgagt aatggtctga ccgtcgatca tgtcagtaaa gcgtacaaaa ccgcttactt
     1741 cggtgataac cggcatggtg tgcgggtccc agtttgcaac ggtttcgccg ccagcaacct
     1801 gttcgccatc gcctttcgcc agtaccgcac cgtaaggtac tttgtagctt tctttggtac
     1861 gaccgaattc gtcgatcagt ttcagttcag tattacggga agtgataacc agtttaccgc
     1921 tggagttcac aaccgacttc acgttgctga gcttgatgct acctttgttt ttcacctgga
     1981 tgctggattc agcagccgca cgagatgccg caccaccgat gtggaacgta cgcatggtca
     2041 gctgtgtacc cggttcaccg atggactgtg ccgcgataac accgattgct tcacctttgt
     2101 tgatgatgtg gccacgcgcc aggtcacgac cgtagcagtg cgcacataca ccaaagtcgg
     2161 tgtcacaaga tacaacagaa cgtactttta ccgcgtcgac agagttctct tccagcaggt
     2221 cacaccactg ttcgtgcagc agcgtgttgc gcggaacgag gatatcagca gtacccggct
     2281 tcagaacgtc ttcagcagtt acacgaccca gtacgcgatc gcgcagcggc tctttaacgt
     2341 caccaccctc gataaccgga gtcatcatga taccttcatg ggtaccacaa tcgtcttcgg
     2401 taaccaccag gtcctgcgcc acgtcaacca gacgacgagt caggtaaccg gagttcgcag
     2461 ttttcagtgc ggtatccgcc agacctttac gagcaccgtg ggtggagatg aagtactgga
     2521 gtacgttcag accttcacgg aagttcgcgg tgattggcgt ttcgatgatg gagccatccg
     2581 gcttcgccat cagaccacgc ataccagcaa gctgacgaat ctgtgccgca gaaccacgcg
     2641 caccggagtc ggccatcatg tagatgctgt tgaaggaaac ctgcttctct tcctgaccgt
     2701 cacggttaat cacggtttca gtttgcaggt tatccatcat cgctttggat acacgatcgt
     2761 tcgccgcagc ccagatatcg ataactttgt tgtagcgttc gcccgcagtt accagaccag
     2821 actggaactg ctcctgaatt tcagcaactt ctgcttctgc ctcggagatg atttcgtgtt
     2881 tcttctccgg gatgaccatg tcatcgatac caacagatgc accagaacgc gctgcatagg
     2941 cgaagccggt gtacatgatc tggtccgcaa aaataacggt cggtttcaga ccgagaatgc
     3001 ggtagcaggt gttcagcatt ttggagattg cttttttacc cagcgcctgg ttgacgatgg
     3061 agtaaggcag acctttcggt acaatcatcc acagaatggc acggccaaca gtcgtgtctt
     3121 tcaggctggt tttcgctact aattcaccgt ttgcatcttt ttcatactcg gtgatacgca
     3181 ctttaacgcg cgcatgcaga gaagccagac cagagcgata cagacgttct gcttctttcg
     3241 ggccagtcag caccatgcct tcgcctttgg cgttaacaca gtcacgggtc atatagtaca
     3301 gacccagaac aacgtcctga gacggaacga tgattggttc gccgttcgcc ggggacagga
     3361 tgttgttggt agacatcatc agcgcacgcg cttccagctg ggcttccagc gtcagcggta
     3421 cgtgaacagc catctggtca ccatcgaagt cggcgttata tgccgcacaa accagcgggt
     3481 gcagctggat agctttacct tcgatcagta ccggttcaaa tgcctggata cccagacggt
     3541 gcagagtcgg tgcacggttc agcagtaccg ggtgttcgcg gataacttcg tccaggatat
     3601 cccaaacgac agcttcttcg cgctcaacca ttttcttcgc agctttaatg gtggtagcaa
     3661 gaccacgcag ttccagcttg ccgtagatga acggtttgaa cagctccagc gccattttct
     3721 tcggcagacc gcactgatgc agacgcaggt atggacctac ggtgattaca gaacgaccgg
     3781 agtagtcaac acgcttaccg agcaggttct gacggaaacg accctgttta cctttgatca
     3841 tgtcggccaa agatttcaga ggacgcttgt tagaaccggt gatcgcacga ccgcgacgac
     3901 cgttatccag cagggcgtct accgcttcct gcagcatacg tttttcgtta cgtacgatga
     3961 tgtccggcgc agccagatcc agcagacgtt tcagacggtt gttacggtta atgacgcgac
     4021 gatacagatc gttcaggtca gaagtcgcga aacgaccacc atccagcgga accagcggac
     4081 gcagatctgg cggcagtacc ggcagaacgg tcaggatcat ccactctggt ttgttaccag
     4141 actgaacgaa cgcttccagc agtttgatac gcttggtcag ctttttacgc ttggtttcgg
     4201 agttggtttc gttcagctct tcacgcagct gttcgcactc ttgctccaga tccatgctct
     4261 tcagcagagc ctggattgct tccgcaccca tcttcgcgtc gaattcgtca ccgaactctt
     4321 ccagcgcgtc cagatactgc tcttcagtca ggatctgctg acgttccagg ttggtcatac
     4381 cgccttcgat aaccacatag gattcaaagt acagtacgcg ttcgatatcg cgcagcggca
     4441 tatcgagcag cagaccgata cgggacggca gcgatttcag gaaccagatg tgcgcagtcg
     4501 gggaagccag ttcgatgtgg cccatacgct cacggcgtac tttagtctgg gtcacttcaa
     4561 cgccgcactt ctcacagatg acgccacggt gtttcaggcg cttgtactta ccgcacaggc
     4621 actcgtaatc ttttaccggc ccaaagatac gggcgcagaa aaggccgtca cgttctggtt
     4681 tgaacgtacg gtagttgatg gtttccggct ttttaacttc accgaaagac catgaacgga
     4741 tcatgtctgg cgaagccaga gcaattttga tcgcatcaaa ctcttcggtt ttagtctgcg
     4801 ctttcagaaa ctttaataaa tctttcacgg atttgctccc gtcggagtta gcacaatccg
     4861 ctgccgggtt ttaccccgac agcagtgacc tgtttgagcg agaattactc gtcttccagt
     4921 tcgatgttga tacccagcga acgaatctct ttcaacaata cgttgaagga ttctggcatg
     4981 cccggctcca tctgatggtt gccgtccacg atgtttttat acatcttggt acgaccgttc
     5041 acgtcatcag acttaacggt gagcatttcc tgcagggtgt atgctgcgcc gtatgcttcc
     5101 agcgcccaca cttccatctc cccgaaacgc tgaccaccga actgtgcctt accacccagc
     5161 ggctgctgag taaccaggct gtaagaaccg gtggaacgcg cgtgcatctt gtcgtcgacc
     5221 aggtggttca gtttcagcat gtacatgtaa ccaacggtta ccggacgctc gaactgttca
     5281 ccagtgcgac catcgtacag gcggatctga ccggaagtcg gcaggtcgcc aagtttcagc
     5341 agctctttaa tttctgcttc tttcgcaccg tcgaacaccg gcgttgcgat tggcatacct
     5401 ttgcgcaggt tttcagccag acgcataact tcttcatcgc tgaaggtact caggtcaact
     5461 ttctgacgaa cgtcagcgcc cagatcgtac gcacgctgga tgaattcgcg cagtttcgcg
     5521 acttcttgct gctgtttcag catggcgttg atcttgtcgc cgataccttt cgcagccata
     5581 cccaggtggg tttcgaggat ctgaccgatg ttcatacgag acggtacgcc cagcgggttc
     5641 agtacgatgt ctaccggcgt accgttttca tcgtaaggca tatcttcgat cgggttgatc
     5701 ttagaaatta cacccttgtt accgtgacga cctgccatct tgtcaccagg ctggatacgg
     5761 cgtttaaccg ccagatatac cttaacaatc ttcagcacgc ccggtgccag atcgtcgccc
     5821 tgggtgattt tgcggcgttt cgcttcgagt ttcttctcga actcgtgttt cagttcgtca
     5881 tactgctcag ccagctgttc cagctgattt tgtttctctt cgtctgtcag gcctagctcc
     5941 agccagcgat cgcgcggcag tttgtcgagc ttctcagctt caacgccacc ggctaccagc
     6001 acagcacgga tacggctgaa cagacccgct tcgaggatct gcagttcttc agacaggtct
     6061 ttcttcgcct gtttgagctg catttcttcg atttccagcg cacgtttgtc tttttctacg
     6121 ccatcgcgag taaagacctg aacgtcgata accgtaccgg atacaccgtt tggtacgcgc
     6181 agagaagagt ctttaacgtc agaggctttc tcaccgaaga tcgcacgcag cagtttttct
     6241 tctggggtca gctgagtttc acctttcggc gttaccttac caaccagaat gtcgccaccg
     6301 gtcacttccg caccaatgta aacgataccg gattcatcca gtttggagag cgcagcttca
     6361 cccacgttcg ggatgtcagc ggtgatctct tccggcccca gcttggtgtc acgggacaca
     6421 cacgccagtt cctgaatgtg gatggtggtg aaacggtctt cctgaacaac acgctcggat
     6481 acgaggatgg agtcttcgaa gttgtaacca ttccacggca tgaacgctac gcgcatgttc
     6541 tgaccaagcg ccagttcacc gaggtcggtg gacggaccgt ctgccagcac gtcgccacgt
     6601 tcaaccggtt cacccagaga cacacacggc atctggttga tacaggtgtt ctggttagaa
     6661 cgggtgtatt tggtcaggtt gtagatgtcg atacctgctt cacccggata catctcgtct
     6721 tcgttaactt tgataacgat acgggaagca tccacgtact gaacgacacc accacgttta
     6781 gctaccgcag ttacaccgga gtcaacggca acagcacgtt ccataccagt accaaccagc
     6841 ggcttatcag cgcgcagagt cggaacggcc tgacgttgca tgttcgcacc catcaatgca
     6901 cggttggcgt catcgtgttc caggaacggg atcagggacg caccgacgga taccacctgc
     6961 tgggtggata cgtccatgta gtcaacctgg tcgcggctga acaagctgga ttcgcctttg
     7021 ctacggcaag ttaccaggtc ttctacgaag tggccttctt catccaggtt ggagttcgcc
     7081 tgggcgataa cgtagttgcc ttcttcgata gcagacaggt agtgaatttc gtcagttaca
     7141 acaccgtcgg tcactttacg atacggagtc tcaaggaagc cgtattcgtt agtctgtgcg
     7201 tacacggaca gagagttgat cagaccgatg ttcggacctt caggggtttc gattggacat
     7261 acgcgaccgt agtgagtcgg gtgtacgtct cgaacttcga agcctgcacg ttcacgggtc
     7321 agaccgcctg ggccgagtgc ggagatacga cgtttgtgcg taatctcaga cagcgggttg
     7381 ttctggtcca taaactgaga cagctggctg gaaccgaaga actctttcac tgctgcggaa
     7441 atcggcttgg cgttgatcat atcctgaggc atcagggtat ccagatcgcc cagagacaga
     7501 cgctctttca ccgcacgctc tacacgtacc aggccaacgc ggaactggtt ttccgccatt
     7561 tcgccaacgg aacggatacg acggttgccg aggtggtcga tatcatcgac ttcgccttta
     7621 ccgttacgga tatcgatgag ctttttcata acatcaatga tgtcgtcttt gctcaggata
     7681 ccggaacctt cgatttcttc gcgcagcaga gaacggttga acttcatacg accaaccgca
     7741 gacaagtcat aacggtcttc ggagaagaac aggttctcga acaggctttc agctgcttca
     7801 cgagtcggcg gctcgccagg gcgcatcatg cggtagattt ctaccagtgc gctcagacgg
     7861 tcgttagttg ggtcgacacg taaggtttca gagatatatg ggccgtgatc cagatcgttg
     7921 gtgaacagcg tttcgatacg cttgtgacca gactggctca gcttagccag cagatccagg
     7981 ctcagctcca tgttcgctgc gcagatcagc tcgccggtag actcatcaat atagtcttta
     8041 gcaaccactt tacctgcgat gtactcaacc gggacttcga tcagtttgac gtcgtctttt
     8101 tccagctggc gaatgtggcg cgcagtgata cggcggcctt tttctacgta cactttaccg
     8161 ttagcttcga tgtcaaaaga tgcggtttca ccacgcaggc gttccggcac cagttccatc
     8221 tgcagcttgt tatcacggat ttcaaagata actttttcaa agaacaggtc gaggatctgc
     8281 tctgtggtgt agttcagggc gcgcagaatg atggtcgcag gcagtttacg gcgacggtcg
     8341 atacgtacga acaggttgtc cttcggatcg aattcgaagt ccagccagga accacggtaa
     8401 gggatgatac gcgcgttata cagcacttta cccgaagagt gggttttacc tttgtcggag
     8461 tcaaagaaga cgcccggact acggtgcagc tgggaaacga taacacgctc agtaccgttg
     8521 ataacaaagg taccgttgtc tgtcatgagc ggaatttcgc ccatgtagac ttcttgttct
     8581 ttaatgtctt ttacggtgcc ttccggcgct tcgcgctcat agatcaccag acgcagttta
     8641 acgcgcagcg gtgcggaata ggtcacgcca cggatttgac attcctggac gtcaaacacc
     8701 ggttcgccaa ggcggtagct gacgtattgc agctcggaat taccgctgta gctctgaatc
     8761 gggaatacgg aacggaaagc agcttccaga ccatactgcc cttcaggatc ttgctcgata
     8821 aatttctgaa acgagtcaag ctggatagaa aggagataag gtacatccag aacttgtgga
     8881 cgtttaccaa aatccttacg aatacgtttt ttctcggtat aggagtaaac catagggttc
     8941 ctcagctcgc tgacaagtcg acccatctgt ccattgagcg gacagtttgt gcaacactat
     9001 tttgttgacc ggaaaatgga acactttccg caatgcctgt tgctatcacg cttaaaccat
     9061 ttcattgcga tttacacaga acggacgtcc tgtcgcagta tattaagtcg tcgatagaaa
     9121 caagcattga aaggcacagc agtagtcaaa cagtgtgaaa cgctactggc gccttacagc
     9181 gcaaaaaggc tggtgactaa aaagtcacca gccatcagcc tgatttctca ggctgcaacc
     9241 ggaagggttg gcttatttaa cttcaacttc agcgccagct tcttccagag cttttttcag
     9301 tgcttctgcg tcgtctttgc tcacgccttc tttcagagca gccggtgcag attctaccag
     9361 gtctttagct tctttcagac ccaggccagt tgcgccacgt actgctttga taacagcaac
     9421 tttgttagcg ccagcagctt tcagaattac gtcgaattca gttttttctt cagcagcttc
     9481 aaccgggcca gcagctacag ctacagcagc agcagcggaa acaccgaatt tttcttccat
     9541 tgcagagatc agttctacaa cgtccattac agacatagct gcaactgctt caatgatttg
     9601 atctttagtg atagacattt aaattgttcc tgaatatcag aataagttta tacgtaagcg
     9661 aatgcgttaa aaagataact gcgattaagc agcgtctttc gcatcgcgta cagcagccag
     9721 agtacgaacc agtttgccag ccgaagcttc tttcatggtt gccatcaggc gtgcaattgc
     9781 ttcttcgtag gtcggcagag ttgccaggcg gtcgatctga gacgccggga tcagctcacc
     9841 ttcaaaggca gcggctttga cctcaaattt tgcattcgct ttcgcgaact ctttgaacag
     9901 acgagcagca gcgcccgggt gttccataga gtatgcaatc agggtcggac caacaaacgc
     9961 gtctttcagg cactcgaacg gagtaccttc aacagcacgg cgcagcaggg tgttacgaac
    10021 aacacgcatg tatacgccag cttcgcgacc tgctttacgc agttcagtca ttttatctac
    10081 agttacgcca cgggaatccg caactactgc agacagcgcg cctttggcta cttcgctgac
    10141 ttcagcaaca atcgcttgtt tgtcttgaag atttaaagcc attagctttg ctcctggatg
    10201 tttgccagag aaaatctctg gaactcactt cactcctcca aacggagaga gcgtcttaat
    10261 tacggtgagc agaaacaagc cagaatataa aagaataatc ttagcgttct gtcaccgtct
    10321 acgcagggga ttaagtttct tgcgaaactc ctgcggtctt cgacggaggc ctggataggc
    10381 caggctccaa cgaacaaatc ttttctatcc gtcgctattc aacttgtggc ggcgttggct
    10441 tccttcattc gcaccagtca catagttagc tatgctcctg gcgactcact cagttgccgc
    10501 cttgccacaa cccgaatatc tcggatatac gtctgcatta agcattatac gtgggggtaa
    10561 gattgtagac aaaatcaccg cccacgtaaa ggcattagtt tacagaagcg ctcaggccag
    10621 cctggtcaac tgcaacacct gcacccatgg tggtggagat gctaactttc ttgatgtaca
    10681 cgcctttcgc ctgagtcggt tttgcttttt tcagcgcaac cagcagagct tccaggtttt
    10741 ctttcagttt gtcagcgtca aagtccactt taccgatggt ggtgtggatg atgccgtttt
    10801 tgtcgttacg gtaacgaacc tggccagctt tagcgttttt aaccgcttca gcaacgttcg
    10861 gtgttacagt acccactttc gggtttggca tcaggccgcg cggacccaga acctggccca
    10921 gctggccaac aacgcgcatt gcatccggag aagcaataac aacgtcaaag ttcatttcgc
    10981 ctttcttgat ctggtcagcc agatcttcca tacctaccag ttctgcgcct gcagctttag
    11041 cagcttcagc gtttgcacct tgggtaaata cggctacgcg aacggaacgg ccagtaccgt
    11101 gcggcagtac agttgcacca cgtacgttct ggtcagattt acgagcgtcg atgccgaggt
    11161 taacagctac gtccacgctt tctacgaatt tagcagtcgc cagctctttc agcagtgcga
    11221 tagcttcgtt gatgtcgtac tgtttggttg catcaacttt ctcgcggata acacgcatgc
    11281 gcttggtcag tttagccatt tcttagtcct ccactaccag gcccatggaa cgtgcagtac
    11341 cttcgatgga gcgagtcatc gcttcaatgt cggcaccagt catgtcggca gctttggtct
    11401 gcgcgatttc ctgcagctga gcgcgggaaa ttttacccac tttgtctttg ttcggcttac
    11461 cggaaccaga cttgatacca gccgcttttt tcagcagaac tgctgccggc ggggtcttgg
    11521 taacgaaagt gaaagaacgg tcagcgtaaa cggtgattac taccggaatc ggcagacctt
    11581 tttcgatgga atcagttttt gcgttgaacg ctttgcagaa ttccatgatg tttacgccct
    11641 gctgacccag agccggacct actggcggac tcgggttagc cataccagct gcaacctgca
    11701 gcttgacata ggcttgtact ttcttagcca ttataaattc ctcaagttgg gtaataacgc
    11761 ctctgagagg ctccccgtga tataaaaaat cgttttacgg gcaaggccca taaaaacaaa
    11821 aggcgcgaaa ttgtattcca atctcacgcc ttgtgcaacg attaaatcgc cgcttttttg
    11881 atcgctgggt taggcttttt caacctggct gaagtccagc tctaccgggg tcgcacgacc
    11941 gaagatagaa acagacactt tcagacgaga tttctcgtaa tccacttctt caacaacacc
    12001 gttgaagtca gcgaacggac catcattaac acggaccatt tcacccggtt caaacagcgt
    12061 tttcggacgc ggcttatcac caacctgctg caggcggttc ataatcgcat cgacttcttt
    12121 atcgctgatt ggcgcaggac gatcggaagt accgccgatg aagcccatca cacgcggtac
    12181 gctgcgcacc aggtgccagc tcgcgtcgtt catcaccatc tgaacgagga cgtagccagg
    12241 gaagaattta cgttcgcttt tgcgacgctg accgccacgg atttcaacca cttcttcggt
    12301 tggtaccatg acttcaccaa acaaatcttc catgttgtgt aatttgatat gctcacgcag
    12361 cgacgttgct acgcggcctt caaaaccgga aaacgcctga acgacgtacc agcgcttttt
    12421 aggagcttca gacatctcag aacctcaggc cagtgataaa ggataccagg cgaaccagaa
    12481 taccatccag tccccacagg atcagtgaca ttactgcggt aaccgcagcc acaatcagcg
    12541 tggtgtgcaa tgtttcctgg cgagtcggcc aaatgacctt acggacttcg gtacgcgctt
    12601 cacgggcaaa agcaacggta gctttacctt ttgtcgttaa cagcgcgaca ccacccgctg
    12661 cagcaatcag aattactacg gccagcgcac gcagcggcag cataatgtcg cgataaaggt
    12721 agttgccgac aatcgccacc aggagcaatg ccaccacaac gacccacttc atcgcttcca
    12781 ggccgcgccc gcttccttga gcttcggtat tcgcactcat aaaccaacct gtcagaagtt
    12841 ttctacaaac attttcaccc cgcgatcgcg aggcaaacca aatcgaaacg cgtattagct
    12901 tttcggatta tacgccctca acagagcctg tctcagcaat gattatgaca aagaaaatca
    12961 ctgatgagcc aggttctggt acgaaagcgt gcaaaaaggg catcaaatga tgccctttta
    13021 gtgcgcattg cgtcaaatgt tatcggcaat tagctcagaa cttttgctac aacgcccgcg
    13081 ccaacggtac ggccgccttc acggattgcg aaacgcagac cgtcgtccat cgcgatcggg
    13141 tggatcaggg taacaaccat tttgatgttg tcgcccggca ttaccatctc tacgccttcc
    13201 ggcagttcga tggtaccagt cacgtcagta gtacggaagt agaactgcgg acggtagcct
    13261 ttgaagaacg gagtatgacg
//
LOCUS       DAZGSP010000133        13240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZGSP010000133 DAZGSP010000000
VERSION     DAZGSP010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.134"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..297
                     /locus_tag="ACHK74_004419"
     CDS             <1..297
                     /locus_tag="ACHK74_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HFP0223124.1"
                     /translation="QGFIRFHIPLWVRRTVTMLPSFIVILMGLDPTRILVMSQVLLSF
                     GIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWVIVVLVVALNIWLLVGTALGL"
     gene            complement(438..764)
                     /gene="ypeC"
                     /locus_tag="ACHK74_004420"
     CDS             complement(438..764)
                     /gene="ypeC"
                     /locus_tag="ACHK74_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFP0223125.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(879..2135)
                     /locus_tag="ACHK74_004421"
     CDS             complement(879..2135)
                     /locus_tag="ACHK74_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFP0223126.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASALQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            2339..3304
                     /gene="glk"
                     /locus_tag="ACHK74_004422"
     CDS             2339..3304
                     /gene="glk"
                     /locus_tag="ACHK74_004422"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFP0223127.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            3523..3849
                     /locus_tag="ACHK74_004423"
     CDS             3523..3849
                     /locus_tag="ACHK74_004423"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFP0223128.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAVGIIKEIEEM
                     IASEQQ"
     gene            3871..5118
                     /locus_tag="ACHK74_004424"
     CDS             3871..5118
                     /locus_tag="ACHK74_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP0223129.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            5133..6218
                     /gene="ypdF"
                     /locus_tag="ACHK74_004425"
     CDS             5133..6218
                     /gene="ypdF"
                     /locus_tag="ACHK74_004425"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP0223130.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            6218..7255
                     /gene="ypdE"
                     /locus_tag="ACHK74_004426"
     CDS             6218..7255
                     /gene="ypdE"
                     /locus_tag="ACHK74_004426"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP0223131.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            7280..9775
                     /gene="ptsP"
                     /locus_tag="ACHK74_004427"
     CDS             7280..9775
                     /gene="ptsP"
                     /locus_tag="ACHK74_004427"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP0223132.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQVRRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9778..10635)
                     /locus_tag="ACHK74_004428"
     CDS             complement(9778..10635)
                     /locus_tag="ACHK74_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0223133.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(10648..11382)
                     /gene="ypdB"
                     /locus_tag="ACHK74_004429"
     CDS             complement(10648..11382)
                     /gene="ypdB"
                     /locus_tag="ACHK74_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFP0223134.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(11397..13094)
                     /gene="ypdA"
                     /locus_tag="ACHK74_004430"
     CDS             complement(11397..13094)
                     /gene="ypdA"
                     /locus_tag="ACHK74_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFP0223135.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT         3118 a         3393 c         3605 g         3124 t
ORIGIN      
        1 cagggattca ttcgcttcca tatcccgctg tgggtgcgtc gtacagtcac catgttgccg
       61 tcatttattg tcattctgat gggattagat ccgacacgga ttctggttat gagtcaggtg
      121 ctgttaagtt ttggtatcgc cctggcgctg gttccactgc tgattttcac cagtgacagc
      181 aagttgatgg gcgatctggt gaacagcaaa cgcgtaaaac agacaggctg ggtgattgta
      241 gtgctggtcg tggcgctgaa tatctggttg ttggtgggga cggcgctggg attgtagttg
      301 aatgagcgtc gcatctggca ctattggcgg atgcggcgta aacgccttat ccgccctacg
      361 cgttaaagag ccggtttgta ggcctgataa gactcgctag cgtcgcatca ggcattgtgt
      421 tccattggcg aatgtgttta atggcggtgt ccatgtccgc ggccttttcc gcgatgatcg
      481 tcacgatcgc gccagccttc acgatagcca cgctcatacg ctttacgctt atcccagcca
      541 cgatgatagc cattatcatg acgccaccag cggtttttac gccactcata attgcgatgc
      601 cagtagtcac ggtcgcgcca gtgaccaccg tcccagtaat taccgtaatg atcgcgatcg
      661 ccaatttgta atttgattga tggcagtagg gtgatttcac ctgcgtttgc tgcaagcggg
      721 gtaaatgcca tcagggcggc cgccagaaac agtgacctga acattgttat tctccttcac
      781 gatcgaagcc gtcagcggcc tgttaacgca atattacggg gaggtaaagc cccgtatcat
      841 cgccataact cttaaatcat aaaggagagc attttttgct aaacgttatc gtggggtggc
      901 tgttgcttcg gacgattgac catcatcatc ggcttacctg ctaataacag ccatgccgga
      961 agcatgacga tacagagcag cggtagcaat gtggtattgg gtacaacgac cgccgccata
     1021 aaaagactta accagccatc gcgtgttacc accagcacga tgccgagaat agcgcaagaa
     1081 acggttattg ccgctggtac ggcgggtacg tgctcatgca gcatcaaccc taatgccacg
     1141 ccgacaaaca ctgccgggaa gattcgccca ccgcgaaagc cactggcggc agcaacgacc
     1201 agggcggcaa gtttaattac cgccagcaaa aagtaatcgc tggtgctgaa agcctgattt
     1261 gccaccatct gctgcatctc atccagccct ttaaacagcg aaactggtcc accaataacc
     1321 cccagaatac cgagaataaa tccgccaata cccagcacga gcaccggatt tttcatttga
     1381 ttcatcatcg cgtgcaaccg tggtaagcac catacggcga ccatccctgc tgcgatggca
     1441 atcgccgcga caattgcacc gctgagaata tcggtcattt ccatctgtcc gtaatgcgca
     1501 atgggcagtg aaaaatgggg atggaaaaat aatccggtag taagtgcacc agctgctgcc
     1561 gccattaatg gcgcaaaaag acgatcccat agcggaactt cactactgcc atttaaggtt
     1621 tgcgaaaata tcaacgccgc cgcaacaggt gtaccaaaca gcgcgccgat ggttccggca
     1681 gaggctaaaa tagtccactc cattcggttg acgcgcggta acagacgagc gccaatggca
     1741 accgcaaggg cgatattgac ggtcatgatc ggatgttccg gccccaggct gacgccgcca
     1801 gcaagaccga gaattaatgc tacgataagt cctggtagcg cagagggcgg aactggtgcg
     1861 ccgatcagcg gttcacaggc ggggtctggt ccggcatgac cctggctgaa acggataacc
     1921 aaccccaccg caatacccgt tagcgttaat acaccgatga tccagagagg tgaatcctgg
     1981 gctatcccca gagttcccgg cagtcgttgc cagagcaaat tctgtaatgc cgaggcgatt
     2041 ttcatcacca caatcagaat aagactggac gcaatcccaa ttgccacggc ggggagcgat
     2101 aataacaaca tggttctggc tcgcggatgg agcatgttct attccttatg cggggtcaga
     2161 tacttagttt gcccagcttg caaaaaggca tcgctgcaat tggtgctgaa acgataaagt
     2221 aaatgtgtga cccagatcga tatttacagg gagcctgcct ttccggcgtt gttgttatgc
     2281 ccccaggtat ttacagtgtg agaaagaatt attttgactt tagcggagca gttgaagaat
     2341 gacaaagtat gcattagtcg gtgatgtggg cggcaccaac gcacgtcttg ctctgtgtga
     2401 tattgccagt ggtgaaatct cgcaggctaa gacctattca gggcttgatt accccagcct
     2461 cgaagcggtc attcgcgttt atcttgaaga acataaggtc gaggtgaaag acggctgtat
     2521 tgccatcgct tgcccaatta ccggtgactg ggtggcaatg accaaccata cctgggcgtt
     2581 ctcaattgcc gaaatgaaaa agaatctcgg ttttagccat ctggaaatta ttaacgattt
     2641 taccgctgta tcgatggcga tcccgatgct gaaaaaagag catctgattc agtttggtgg
     2701 cgcagaaccg gtagaaggta agcctattgc ggtttacggt gccggaacgg ggcttggggt
     2761 tgcgcatctg gtccatgtcg ataagcgttg ggtaagcttg ccgggcgaag gcggtcacgt
     2821 tgattttgcg ccgaatagtg aagaagaggc cattatcctc gaaatactgc gtgcggaaat
     2881 tggccatgtt tcggcggaac gcgtgctttc tggccctggg ctggtgaatt tgtatcgcgc
     2941 aattgtgaaa gctgacaacc gcctgccaga aaatctcaag ccaaaagata ttaccgaacg
     3001 tgcgctggct gatagctgca ccgattgccg ccgcgcgttg tcgctgtttt gcgtcattat
     3061 gggccgtttt ggcggcaatc tggcgctcaa tctcgggaca tttggcggcg tgtttattgc
     3121 gggcggtatc gtgccgcgct tccttgagtt cttcaaagcc tctggtttcc gtgccgcatt
     3181 tgaagataaa gggcgcttta aagaatatgt ccatgatatt ccggtgtatc tcatcgtcca
     3241 tgacaatccg ggtcttctcg gttccggtgc acatttacgc cagaccttag gtcacattct
     3301 gtaaatcctt cctgttatat cgggaggtaa ctctcccgat aatcttttaa atcatacagt
     3361 taattcaatt tttctttgtg tcccctcaca aggccgacct gcgtcacact tccatacagc
     3421 gggattaatt ctccagtaaa tgcattattt gtctggtaac ggcgatttgt tttgcacgtt
     3481 cataatttca ctcgtcaact atcactgaac gaggaattaa cgatgagtaa gaaactgatt
     3541 gccttatgtg cctgcccgat gggcctggct cacactttta tggccgctca ggcgctggaa
     3601 gaagcggcgg tagaagccgg ttatgaagtg aaaattgaaa cccagggcgc ggacggcatc
     3661 cagaatcgcc tgacggcgca ggatatcgcc gaagcgacca tcatcatcca ttccgtggca
     3721 gttaccccgg aagataacga acgtttcgaa tcacgcgacg tttatgaaat cactttgcag
     3781 gacgcaatta aaaacgctgt aggcatcatc aaagaaatcg aagagatgat tgcctctgaa
     3841 caacagtaat attcgacagg gataattgtt atggccatta aaaaacgcag tgcaaccgtt
     3901 gtgcctggcg catccggtgc ggcagcggca gttaagaatc cgcaggcctc taaaagcagc
     3961 ttctggggtg aactcccgca gcatgtgatg tcagggattt cacgcatggt gccgacctta
     4021 attatgggcg gtgtgatcct cgctttcagc cagctgattg cttatagctg gcttaaaatt
     4081 cctgctgaga tcggcatcat ggatgctctt aatagcggga aattctccgg tttcgacctc
     4141 tccttactga aatttgcctg gctgtcgcag tccttcggcg gcgtgttgtt tggctttgct
     4201 attccgatgt ttgccgcttt tgtggcgaac tctatcggcg gcaaactggc gttcccggct
     4261 ggttttatcg gcggcttgat gtctacccag ccgacgcaac tgctgaactt cgatcccagc
     4321 actatgcaat gggcgacctc ttcgccggtg ccgtccacct tcattggtgc gctgattata
     4381 tctatcgttg ctggttacct tgtgaaatgg atgaaccaga aaatccagtt accagatttc
     4441 ctgttagcgt tcaaaaccac atttttgcta ccgattcttt ccgccatttt tgtcatgctg
     4501 gcgatgtact atgtcatcac cccatttggt ggctggatca acggcggtat ccgtaccgtg
     4561 ctgactgccg caggtgagaa gggcgcgctg atgtatgcaa tggggattgc cgcggcgacc
     4621 gcaatcgacc ttggtggccc aatcaacaaa gcggcaggtt tcgttgcctt cagctttacc
     4681 accgaccacg tattaccggt taccgcgcgt tctatcgcta tcgttattcc gccgattggt
     4741 ctggggctgg cgaccattat cgaccgtcgt ttaaccggca aacgcctgtt caacgctcaa
     4801 ctttatccgc agggtaagac cgccatgttc ctggcgttta tggggatcag tgagggtgcg
     4861 attccgtttg cgctggaaag ccccatcacc gccattccgt cgtatatggt cggcgctatt
     4921 gtcggctcaa ccgccgctgt ctggctgggc gcagtgcaat ggttcccgga gtccgctatc
     4981 tgggcatggc cgctggtcac taacctgggc gtctatatgg cggggatcgc gctgggggca
     5041 gtcataactg cgctgatggt ggtgttcctg cgtctgatga tgttccgtaa aggcaaattg
     5101 ttaatcgata gcctgtaaga aggaaaatcc agatgacatt actcgcttcg ctgcgcgact
     5161 ggcttaaggc gcaacaactg gatgcagtgc ttctctcctc acggcagaac aaacagccgc
     5221 atctggggat ctccaccgga tcaggctatg tggttattag ccgtgaaagt gcgcacattc
     5281 tggtggattc gcgctattac gttgaggtgg aagcccgtgc gcaaggctac cagctgcatt
     5341 tgcttgacgc gacgaacacg cttaccacta tcgtcaatca aatcattgcc gatgagcagt
     5401 tgcaaacgct cggttttgaa ggccagcagg tgagttggga aaccgcgcac cgctggaagt
     5461 ctgaactcaa tgcgaaactg gttagcgcca cgccggatgt gctgcggcaa atcaaaacgc
     5521 cagaggaggt ggagataatc cgcctcgcct gtgggattgc cgatcgcgga gcggagcata
     5581 ttcgccgctt tattcaggcg gggatgagcg agcgcgagat agccgctgaa ctggagtggt
     5641 ttatgcgcca gcagggcgca gaaaaagcct cttttgacac cattgtcgcc agcggctggc
     5701 gcggggcgct gccgcacggc aaagccagcg acaagattgt tgcagcgggc gagtttgtca
     5761 ctctcgattt tggtgcgctg tatcagggct actgctctga tatgacgcgc accttgctgg
     5821 tgaatggcga aggggtgagc gccgaatctc acccgctgtt tgacgtctat cagattgtcc
     5881 tgcaggcaca gctcgcggca atctctgcaa ttcgccccgg cgtgcgctgc cagcaggttg
     5941 acgatgccgc gcgccaagtg attaccgagg caggttttag ccactatttc ggtcataaca
     6001 ccggtcatgc tatcggcatt gaagttcatg aagatccgcg tttttcaccg cgggacacca
     6061 cgacgctaca gccaggcatg ttactgaccg tggagccggg gatttatttg ccagggcaag
     6121 ggggcgtgcg catcgaagat gttgtgctgg ttaccccgca aggcgcagaa gtgctctacg
     6181 ccatgccgaa aacagtgttg ctcacgggag aggcataatg gatttatcgc tattaaaagc
     6241 gttgagcgag gcagatgcga tcgcctcctc ggaacaggaa gtgcggcaga tcctgctgga
     6301 agaagcggat cgcctgcaaa aagaagtgcg atttgatggt ctgggatcgg tgctgatccg
     6361 cctcaatgaa tcgacaggtc cgaaggtgat gatctgcgcc catatggacg aagtgggatt
     6421 tatggtgcgt agcatctccc gcgaaggggc aattgatgtg ctgccggttg gcaacgtgcg
     6481 catggctgcc cgccagctgc agccggtgcg catcaccacc cgtgaagagt gcaaaattcc
     6541 aggcctgctt gacggcgacc ggcaggggaa tgacgtcagc gccatgcgcg tggacattgg
     6601 tgcgcgctcg tatgacgaag tgatgcaggc gggaattcgt ccaggcgatc gcgtcacctt
     6661 tgatacaact tttcaggttc tccctcacca gcgggtgatg gggaaagcct ttgatgatcg
     6721 cctcggttgc tatctgctgg tgacgttact acgtgagctg cacgatgccg aactgcctgc
     6781 ggaggtgtgg ctggtggcaa gttccagcga agaggtggga ttacgcggtg ggcaaaccgc
     6841 tacccgcgcg gtgtcgccgg acgtcgccat tgtgctggat accgcctgct gggcgaaaaa
     6901 ctttgattat ggcgcggcta accatcgcca gattggtaac ggcccgatgc tggtgttaag
     6961 cgacaagtca ctgattgcgc cgccaaaact taccgcctgg atcgaaaccg tggcggctga
     7021 aattggcgta ccgttgcagg cagatatgtt cagcaacggc ggcacggacg gcggagcggt
     7081 gcacttaacc ggtactggcg tacccacagt ggtaatgggg cctgccaccc gccacggaca
     7141 ttgcgcggca tcgattgccg attgccgcga cattttgcag atggagcaac ttttatctgc
     7201 ccttattcaa cgtcttacgc gtgagacggt tgttcaactg acggatttca gatgatctcc
     7261 tgattaaccc ggagcggtta tgttaacgat tcaatttctc tgtcctttgc ctaacggtct
     7321 acatgctcgt ccggcgtggg aacttaaaga acagtgcagc cagtggcaaa gcgaaatcac
     7381 ctttttcaac catcgccaga acgcaaaggc cgatgctaaa agctcgctgg cgttgattgg
     7441 aactggcacc ttatttaatg acagttgcag cctgaacatt agcggcagcg atgaagagca
     7501 ggtgcggcgc gtgctggaag agtacatcct ggtgcgcttt atcgacagcg acagcgttca
     7561 gccgacgcag gcagaactga cggcgcatcc gctgccgcgt tcattaagcc gccttaaccc
     7621 ggatttactg tacggcaatg tgctggcaag cggcgtcggc gtgggtacgc tgaccctgtt
     7681 acaaagcgac agcctcgaca gttatcgggc aatccccgcc agtgcgcaag attccacccg
     7741 gctggagcac agcctggcaa cgcttgccga gcaactgaac cagcaattgc gtgagcgtga
     7801 cggcgaaagc aaaactatcc tcagcgccca tttgtcgctg attcaggatg atgaattcgc
     7861 gggcaatatc cgtcgtctga tgacagaaca gcatcaaggg ctgggggcgg cgatcatcag
     7921 caatatggag caggtttgcg ccaaactttc tgcttctgcc agtgattatc tgcgcgaacg
     7981 tgttagcgac attcgcgata ttagcgaaca gttgctgcat atcacctggc cggaactgaa
     8041 gccgcgcaac aatctggtgc tggaaaaacc gactattctg gtagccgagg atttaacccc
     8101 aagccagttt ttgagcctcg atttaaaaaa tcttgcaggc atgattctgg agaaaactgg
     8161 gcgcacctcg cataccctga ttctggcccg cgcctcggcg atcccggtac tgagcggcct
     8221 gccgctggat gcgattgccc gttatgccgg gcaaccggcg gtgcttgacg cccagtgcgg
     8281 cgtgctggcg attaacccga atgacgcggt gagcggttat tatcaggtcg cacagacgct
     8341 ggcggataaa cgccaaaaac aacaggcgca ggccgccgcg cagctggcct attcccgtga
     8401 taacaagcgt attgatattg cggcgaatat cggcaccgct ctggaagcgc caggcgcgtt
     8461 tgccaacggc gcggaaggcg ttggactgtt ccgtactgaa atgctctata tggatcgcga
     8521 cagcgcgccg gacgagcagg agcaatttga agcctaccag caggtgctac tggcagcggg
     8581 cgacaagccg attatcttcc gcacaatgga catcggtggc gataaaagca ttccttatct
     8641 gaatattccc caggaagaga acccgttcct cggctatcgc gcggtacgta tttacccgga
     8701 atttgctggc ctgttccgca ctcaactacg ggcgattttg cgcgccgcca gttttggcaa
     8761 cgcccagttg atgatcccga tggtccacag tctcgatcag atcttatggg taaagggcga
     8821 gatccaaaaa gcgatcgttg agcttaagcg cgatggcctg cgtcatgcag agacgattac
     8881 gctggggatc atggtggaag tgccgtcggt gtgctacatc atcgatcact tctgcgatga
     8941 ggtcgatttc ttcagtatcg gctccaacga tatgacccag tatctgtatg cggtcgatcg
     9001 taataacccg cgcgtatcgc cgctgtataa cccgattacg ccatcgttcc tgcgcatgtt
     9061 gcagcagatt gttaccactg cgcatcagcg gggcaaatgg gtaggcattt gcggtgaact
     9121 gggcggtgaa agccgttatc tgccgctact gcttgggctg ggcctggatg agctgagtat
     9181 gagtagcccg cgtattccgg cggtgaaaag ccagcttcgt caactggata gcgaggcgtg
     9241 tcgggaactg gcgcgtcagg catgtgaatg ccgcagtgcg caggaaattg aagcgttact
     9301 caccgccttt acgccggaag aagacgttcg cccactgctg gcgctggaga atatctttgt
     9361 tgatcaggat tttagcaata aagagcaggc gatccagttc ctgtgcggca acctcggcgt
     9421 taacgggcgc actgaacatc cgtttgagct ggaagaagac gtctggcagc gggaagagat
     9481 tgttaccacc ggcgttggtt ttggcgtagc gatcccgcac accaaatctc agtggatccg
     9541 tcattccagt atcagcattg cccggctggc gaaaccgatt gactggcagt cagaaatggg
     9601 cgaagtcgaa ctggtgatca tgctgacgct gggtgctaac gaagggatga atcatgtgaa
     9661 agtcttctcg cagctggcgc gtaaactggt gaataaaaac ttccgccagt cgttgtttgc
     9721 cgcgcaagat gcacaaagta tcctgacgct gctggaaaca gaattaacct tctgacgtta
     9781 gccctgaaaa cgggcgctgt actctcccgg cgtcagacca aactgacgcc ggaaaacgcg
     9841 acaaaaatag tcgctatccg gaaaaccgca acgctgtgcc acttcatgaa ttgacagatg
     9901 atatttctgc aaaatcatcc tcgccttcgc cattcgcacc caacgcacat actcgataaa
     9961 acgcatcgtt ccatgctgag caaacagttt tgacagatga ttgggcgtga tattaaaaaa
    10021 ctgcgccacg ctctcgcggg tgagcggctg ggcataatta tcctgaaccc agttacaaat
    10081 gctgtgatag agaaaatcgc cgcgcggctg cgaattgccc ggcggcatat tcaccacgtt
    10141 acggcaaaga tgcagcaaac ttaacaccag tggctgaatg atattctgct ccagcggcga
    10201 acggctaaga tgggtgaggg cggtaagcat cgcttcacct tcaccgcgtt gcaggctggg
    10261 aagctggatc tggcgagcag gatgcaacaa cgacgtggtg cggctatcgt aaaacgataa
    10321 cccaagccag gtcggtgcga acaccaggct taacagcatc accggtttgt tgttgatcgg
    10381 taaattagcc gcgcgtgccg gaataaacag catttctcct tccaccattc gctgctgctt
    10441 tgcttccagc aggttgccgt attcgccgcg cagcacgata tccagacggg gaaaatcaat
    10501 acataaactg cccaccggta acgaggcagg ctggctggca aaccagacgc gccccagtag
    10561 ttgcgggtta agcaccaggc cgctgaacag atcggcgaaa aattgttgat cggcgggcaa
    10621 gcctggtgct ttcatgaaag ttcccgatta aagatgcatt aactggcgaa attctttcac
    10681 tttgctgcgg ctgaccggca cttcaaaatc cagatctttc agtcgcagaa tgtaggtgtt
    10741 attaaaccac ggttcgattt cgcgtatttt gttcagattg acacaaaatg agcgatggca
    10801 gcggaaaaaa tgcgacggcg gcagtttgct gcaaaattcg gtaatgttca tcggcattac
    10861 gtaggattca cgccgcgtat agacaaaggt cattttctcg tgcgcttcgg cgtaatagat
    10921 atcgttaatt ggcgtgacga ttattcgctc atctttcacc agattaatgg tgtcattctc
    10981 acgcgttacc gtcgcggcag gcgtactgct ggtctgctgt tgttgccagg ccgcttccag
    11041 tttttgcagc atcccggtaa tacgtgactc ctgatacggt ttgagaatgt agtcaaacgc
    11101 ctccagttca aacgcttcta ccgcatgttc tttccacgcg gtgatgaaca caataaacgg
    11161 tttatgggcg aactggctga tgttttgcgc cagcaacacg ccatccagcg acggaatatt
    11221 gatatccaga aaaatggcgt cgacgcggtt atgctgcaaa aacttcaaca cgtccagacc
    11281 gtcgtcaaag gtgccgacaa tctccatctg gctgtgctct ttaattagcc agctcagttc
    11341 ctgttgtgcc aggaattcgt cttcaacaat gatgactttc acaatatcac tccggctcaa
    11401 agcaataacg tagcctgtga ggcgactggg gtgcgttggt taggaatgta aaacgcaatt
    11461 tccgttcctg gttccaggcg gcggatatgc agcccctcgc catacaataa cttcacgcga
    11521 tgatgaacat tcagcaggcc aattttattg cccggcattt cattcgcttc gacccgctca
    11581 atcacctttg gatcgatgcc gtgcccggta tcccgcaccg caatgcgtac ccgattgccg
    11641 cactctgcaa cgctgatggt gacaacgcct ttacctttgc aaggctgaat accgtggaca
    11701 atggcattct ccaccaacgg ctggatcagc aggctgggaa tgcagcaatt cacctcttca
    11761 tcaatatcat agataaccgt cagcttgtcg ccaaagcggg cctgctcaat ggcaatatag
    11821 tctttaattt gatacagctc ttttttgata tcgatttgct catcgtcttt taattcaatg
    11881 ttatagcgca gataacgcga cagattaaag atcagctggc gagcagtatc cggattcaga
    11941 cggattgatg atgaaatagc gttcaaagcg ttaaacagga aatggggatt aattttgctt
    12001 tgcagggcgc gaagctctgc cttatttgcc atttcacgta gctgctcggc gcgtgaaacc
    12061 tccagttggg tggagatgat ttgcgacaga ccgaccgcca tttcctgtaa tgacgaggtg
    12121 atctgatgcg cgtggcagta gtaaattttc agcgttccgg tcacaacgcc tttctcccac
    12181 aatgggatca ccagcatgga atgaatctct ggtgtgcggt gggcttcatc attgttttta
    12241 atgatgattt ttccgtaatt catcgcctga cgggtagtcg ggctaatgaa gtcatcgcca
    12301 ttctgatagt tatgttcacc cacgccaaca taggccagca catgatcggt attggtaatc
    12361 gccaccgcat cggcgtgaat gtcatcgcga ataatttcac aaactttgcg taatgactcg
    12421 ctattgacat ggcgaaacag aggtagcgtt ttgttggcga tatccagcgc cagcttggcc
    12481 tgccgcgcgg cgctggcctc tttttcgccc tcaacgcttt gcaccagaag cacaataaag
    12541 ccgatacaga cgctaccgag gatcattgga atgccgattt tagagacgat atcgatcccc
    12601 aacgcggtag ttggtgccca gacaattacc agaatcatgg tcaacgtctc acacaacatg
    12661 ccgccgagaa taccgacgcg ccagcgctgt gctttgggga ttttcaggtt tatccagcct
    12721 gatatacaac ccgccagaat gctggtgata aagcagggga tcgccgtcac gccgccgata
    12781 tcaattaaat accggtgaat acccgcaatc acgccggtaa tgatgcctac ccacgggcca
    12841 aacagaatcc cgccggacat caccgcgata atacgtacgt tcaccagcga gccttctacg
    12901 ggaacgccgg accaggtgct gaacagggcg aacagcgaga aaatggcggt aacggcgagc
    12961 aattcttttg gggagtgcgc cgacttgtgc aacagttcgc gaaacaggcg gatacggatg
    13021 agaaagaaca ggcagataag cattaacgct gcccgatcga agaccgccag cagcatgttg
    13081 aatatttcgt gcacgggtga ctctgaataa ggattaaacc tctatgatag gtaacctgaa
    13141 ggctgatgac cagcaggccg tttttgagga aaaaccataa atttaaaagg ggattgaaag
    13201 ataaatggtg tattagcaat gtatctcttg ataatgcact
//
LOCUS       DAZGSP010000134        13126 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZGSP010000134 DAZGSP010000000
VERSION     DAZGSP010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.135"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            231..1340
                     /gene="livK"
                     /locus_tag="ACHK74_004431"
     CDS             231..1340
                     /gene="livK"
                     /locus_tag="ACHK74_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFP0223136.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1388..2314
                     /gene="livH"
                     /locus_tag="ACHK74_004432"
     CDS             1388..2314
                     /gene="livH"
                     /locus_tag="ACHK74_004432"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFP0223137.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2311..3588
                     /gene="livM"
                     /locus_tag="ACHK74_004433"
     CDS             2311..3588
                     /gene="livM"
                     /locus_tag="ACHK74_004433"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFP0223138.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3585..4352
                     /gene="livG"
                     /locus_tag="ACHK74_004434"
     CDS             3585..4352
                     /gene="livG"
                     /locus_tag="ACHK74_004434"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFP0223139.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4354..5067
                     /gene="livF"
                     /locus_tag="ACHK74_004435"
     CDS             4354..5067
                     /gene="livF"
                     /locus_tag="ACHK74_004435"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFP0223140.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5463..6779
                     /gene="ugpB"
                     /locus_tag="ACHK74_004436"
     CDS             5463..6779
                     /gene="ugpB"
                     /locus_tag="ACHK74_004436"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFP0223141.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     MDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKNKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6877..7764
                     /gene="ugpA"
                     /locus_tag="ACHK74_004437"
     CDS             6877..7764
                     /gene="ugpA"
                     /locus_tag="ACHK74_004437"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFP0223142.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7761..8606
                     /gene="ugpE"
                     /locus_tag="ACHK74_004438"
     CDS             7761..8606
                     /gene="ugpE"
                     /locus_tag="ACHK74_004438"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFP0223143.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLQMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8608..9678
                     /gene="ugpC"
                     /locus_tag="ACHK74_004439"
     CDS             8608..9678
                     /gene="ugpC"
                     /locus_tag="ACHK74_004439"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFP0223144.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9675..10418
                     /gene="ugpQ"
                     /locus_tag="ACHK74_004440"
     CDS             9675..10418
                     /gene="ugpQ"
                     /locus_tag="ACHK74_004440"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP0223145.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTMRLGCVSLHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10405..10845)
                     /locus_tag="ACHK74_004441"
     CDS             complement(10405..10845)
                     /locus_tag="ACHK74_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFP0223146.1"
                     /translation="MKRLLILTALLPFAGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10966..12699
                     /gene="ggt"
                     /locus_tag="ACHK74_004442"
     CDS             10966..12699
                     /gene="ggt"
                     /locus_tag="ACHK74_004442"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFP0223147.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPVSYGVEEDVFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRTKD
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            complement(12737..13021)
                     /locus_tag="ACHK74_004443"
     CDS             complement(12737..13021)
                     /locus_tag="ACHK74_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFP0223148.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2921 a         3329 c         3753 g         3123 t
ORIGIN      
        1 ttattttatg ctgacaacgg cacttttttc tgtttatcta tcaataaatt cagaatatta
       61 tctgttctta atcgactgaa aaatagggat tttaatcgct attatcacaa aatactgcgc
      121 taaaccctta atcagacagg caaaaacagt gcagtataaa aaaagaacag tctgatttgt
      181 taacacataa aaacaaagca acacaacatc acgaatgggg atttttgact atgaaacgga
      241 atgcgaaaac tatcatcgca gggatgattg cactggcaat ttcacacacc gctatggctg
      301 acgatattaa agtcgccgtt gtcggcgcga tgtccggccc gattgcccag tggggcgata
      361 tggaatttaa cggcgcgcgt caggcgatta aagacattaa tgccaaaggg ggaattaagg
      421 gcgacaagct ggtgggcgtg gaatatgacg acgcctgcga cccgaaacaa gccgttgcgg
      481 tcgccaacaa aatcgttaac gacggcatta aatacgttat tggtcatctg tgttcttctt
      541 ctacccagcc tgcgtcagat atctatgaag atgaaggtat tctgatgatc tcgccgggag
      601 caaccaaccc ggagctgacc caacgcggtt atcaacacat tatgcgtact gccgggctgg
      661 actcttccca ggggccaacg gcggcaaaat acattcttga gacggtgaag ccccagcgca
      721 tcgccatcat tcacgacaaa caacagtatg gcgaagggct ggcacgttcg gtgcaggacg
      781 ggctgaaagc ggctaacgcc aatgttgttt tcttcgacgg cattaccgct ggcgagaaag
      841 atttctccgc gctgatcgcc cgcctgaaaa aagaaaacat cgacttcgtt tactacggcg
      901 gttactaccc ggaaatgggg cagatgctgc gccaggcccg ttccgttggc ctgaaaaccc
      961 agtttatggg gccggaaggt gtgggtaatg cgtcgttgtc gaatattgcc ggtgatgccg
     1021 ccgaaggcat gttggtcact atgccaaaac gctatgacca ggatccggca aaccagggca
     1081 tcgttgatgc gctgaaagca gacaagaaag atccgtccgg gccttatgtc tggatcacct
     1141 acgcggcggt gcaatctctg gcaactgccc ttgagcgtac cggcagcgat gagccgctgg
     1201 cgctggtgaa agatttaaaa gctaacggtg caaacaccgt gattgggccg ctgaactggg
     1261 atgaaaaagg cgatcttaag ggatttgatt ttggtgtctt ccagtggcac gccgacggtt
     1321 catccacggc agccaagtga tcatcccacc gcccgtgaaa tgcgggcggg tttagaaagg
     1381 ttaccttatg tctgagcagt ttttgtattt cttgcagcag atgtttaacg gcgtcacgct
     1441 gggcagtacc tatgcgctga tagccatcgg ctacaccatg gtttacggca ttatcggcat
     1501 gataaacttc gcccacggcg aggtttatat gattggcagc tacgtctcat ttatgatcat
     1561 cgccgcgctg atgatgatgg gcattgatac cggctggctg ctggtggcgg cgggattcgt
     1621 cggcgcaatc gtcattgcca gcgcctacgg ctggagtatc gaacgggtgg cctatcgccc
     1681 ggtgcgtaat tctaagcgcc tgattgcgct catctcagca atcggtatgt ccatcttcct
     1741 gcaaaactac gtcagcctga ctgaaggttc gcgcgacgtg gcgctgccga gcctgtttaa
     1801 cgggcagtgg gtggtgggcc atagcgaaaa cttctctgcc tctattacca ccatgcaggc
     1861 ggtgatctgg attgtcacct tcctcgccat gctggcgctg acgattttca ttcgctattc
     1921 ccgcatgggg cgcgcctgtc gtgcctgcgc ggaagatctg aaaatggcga gcctgcttgg
     1981 catcaacacc gaccgggtga ttgcgctgac ctttgtgatt ggcgcagcga tggcggcggt
     2041 ggcaggtgtg ctgctcggtc agttctacgg cgtcattaac ccctacatcg gctttatggc
     2101 cgggatgaaa gcctttaccg ctgcggtgct cggtgggatt ggcagcattc cgggagcgat
     2161 gattggcggg ctgattctgg ggattgcgga ggcgctctct tctgcctatc tgagtacgga
     2221 atataaggat gtggtttcat ttgccctgct gattctggtg ctgctggtga tgccgaccgg
     2281 tattctgggt cgcccggagg tagagaaagt atgaaaccga tgcatattgc aatggcgttg
     2341 ctctctgccg cgatgttttt tgtcctggcg ggcgtcttta tgggcgtaca actggagctg
     2401 gatggcacca aactggtggt cgacacggct tcagacatcc gttggcagtg ggtgtttatc
     2461 ggcacggcgg tggtcttttt cttccagctt ttgcgaccgg ctttccagaa agggttgaaa
     2521 agcgtttccg gaccgaagtt tattctgccc gccattgatg gctccacggt gaagcagaaa
     2581 ctattcctcg tggcgctgct ggtgcttgcg gtggcgtggc cgtttatggt gtcgcgtggg
     2641 acggtggata tcgccaccct gaccatgatc tacattatcc tcggcctcgg gctgaacgtg
     2701 gttgttggtc tttctggtct gctggtgctg gggtacggcg gcttttacgc catcggcgct
     2761 tacacctttg cgctgctcaa tcactattat ggtctgggct tctggacctg tctgccgatt
     2821 gccgggttaa tggcggcagc ggcgggcttc ctgctcggtt ttccggtgct gcgtctacgc
     2881 ggtgactatc tggcgatcgt taccctcggt ttcggcgaaa ttgtgcgcat attgctgctc
     2941 aataacaccg aaattaccgg cggcccgaac ggaatcagtc agatcccgaa accgactttc
     3001 ttcggccttg agttcagccg taccgctcgt gaaggcggct gggacacgtt cagtaatttc
     3061 tttggcctga aatacgatcc ctccgatcgc gtcatcttcc tgtatctggt ggcgttgctg
     3121 ctggtggtgc taagcctgtt tgtcattaac cgcctgctgc ggatgccgct ggggcgtgcg
     3181 tgggaagcat tgcgtgaaga tgaaattgcc tgccgttcgc tgggcttaag cccgcgtcgt
     3241 atcaagctga ccgcctttac catcagcgcc gcgtttgccg gttttgccgg aacgctgttt
     3301 gctgcgcgcc agggctttgt cagcccggaa tccttcacct ttgctgaatc ggcgtttgta
     3361 ctggcgatag tggtgctcgg cggtatggga tcgcagtttg cggtgattct ggcggcaatt
     3421 ttgctggtgg tatcgcgcga attgatgcgt gatttcaacg aatacagcat gttaatgctc
     3481 ggtggtttga tggtgctgat gatgatctgg cgtccgcagg gcttgctgcc catgacgcgc
     3541 ccgcaactga agctgaaaaa cggcgcagcg aaaggagagc aggcatgagt cagccattat
     3601 tatctgtgaa cggcctgatg atgcgcttcg gcggcctgct ggcggtgaac aacgtcaatc
     3661 ttgaactgta cccgcaggag atcgtctcgt taatcggccc taacggtgcc ggaaaaacca
     3721 cggtttttaa ctgcctgacc ggtttctaca aacccaccgg cggcaccatt ttactgcgcg
     3781 atcagcatct tgaaggttta ccggggcagc aaattgcccg catgggcgtg gtgcgcacct
     3841 tccagcatgt gcgtctgttc cgtgaaatga cggtaattga aaacctgctg gtggcgcagc
     3901 atcaacaact gaaaaccggg ctgttctctg gcctgttgaa aacgccttct ttccgtcgcg
     3961 cccagagcga agcgctcgac cgcgccgcca cctggcttga gcgcattggt ttgctggaac
     4021 acgccaaccg tcaggcgagt aacctggcct atggtgacca acgccgtctg gagattgccc
     4081 gctgcatggt gacgcagccg gagattttaa tgctcgacga accggcggca ggtcttaacc
     4141 caaaagaaac caaagagctg gatgagctga ttgccgaact gcgcaatcat cacaacacca
     4201 ctatcttgtt gattgaacac gatatgaagc tggtgatggg gatttcagac cgaatttatg
     4261 tggtcaatca ggggacgcca ctggcaaacg gtacgccgga gcaaatccgt aataacccgg
     4321 acgtgatccg tgcctattta ggtgaggcat aagatggaaa aagtcatgtt gtcctttgac
     4381 aaagtcagcg cccactacgg caaaatccag gcgctgcatg aggtgagcct gcatatcaat
     4441 cagggcgaga ttgtcaccct cattggcgcg aacggggcgg ggaaaaccac attgctcggc
     4501 acgttatgcg gcgatccgcg cgctaccagc gggcgaattg tgtttgatga taaagacatt
     4561 accgactggc agacggcgaa aatcatgcgc gaagcggtag cgattgtccc ggaagggcgt
     4621 cgcgtcttct cgcggatgac ggtggaagag aacctggcga tgggcggctt ttttgccgaa
     4681 cgcgaccagt tccaggagcg cataaagtgg gtgtatgaac tgtttccacg tctgcatgag
     4741 cgccgtgttc agcgggcagg caccatgtcc ggcggtgagc aacaaatgct ggcgattggt
     4801 cgcgcgctga tgagcaaccc gcgtttgctg ctacttgatg agccatcgct cggtcttgcg
     4861 ccgattatca tccagcaaat tttcgacacc atcgagcagc tgcgcgagca ggggatgact
     4921 atcttcctcg tcgagcagaa cgccaaccag gcgctaaagc tggcggatcg cggctacgtg
     4981 ctggaaaacg gccatgtggt gctctccgat accggcgacg cgctgctggc gaacgaagca
     5041 gtgagaagtg cgtatttagg cgggtaataa cacgttgatt gataaggagt caaaagactc
     5101 ctttgagaca ggtgacaaat gtaaaattgc ctgatgcgct gcgcttatca ggcctactgg
     5161 gtgagttgca atatattgaa tttgcacgat cttgtaggcc ggataaggcg tttacgccgc
     5221 atccggcatg aaacgatgag taatctgtag agtttgattc agaccttcta ttttccgctt
     5281 atccgtgccc catctcccat tttccctcac ccacgccgtc accgccttgt catctttctg
     5341 acaccttact atcttacaaa tgtaacaaaa aagttatttt tctgtaattc gagcatgtca
     5401 tgttaccccg cgagcataaa acgcgtgaat tcgcgcattc ggtacaacaa gagagataaa
     5461 cgatgaaacc gttacattat acagcttcag cactggcgct cggactggcg ttaatgggga
     5521 atgcacaggc agtgacgacc attccgttct ggcattctat ggaaggggaa ctgggtaaag
     5581 aggtggattc tctggcccaa cgttttaacg ccgaaaatcc ggattacaaa attgtaccga
     5641 cctataaagg caactacgaa cagaatttaa gcgcggggat tgccgcattt cgtaccggca
     5701 acgctccggc tattttgcag gtttatgaag ttggcaccgc caccatgatg gcatcgaaag
     5761 ccattaaacc ggtatatgac gtgtttaaag aggcggggat tcaattcgat gagtcgcagt
     5821 ttgtgccgac ggtttccggt tactactccg acagcaaaac gggccactta ctctcccagc
     5881 cattcaacag ctcgaccccc gttctctatt acaacaaaga cgccttcaag aaagcaggat
     5941 tagacccgga acaaccgccg aaaacctggc aggatctggc ggactatgcc gcgaaactga
     6001 aagcctccgg tatgaagtgc ggctacgcca gcggctggca gggctggatc caactggaaa
     6061 actttagcgc ctggaacggt ctgccgtttg ccagcaaaaa caacggcttt gacggcatgg
     6121 acgcggtgct ggagttcaac aagccggagc aggtgaaaca catcgccatg ctcgaggaga
     6181 tgaacaagaa gggcgacttc agctacgtcg gtcgtaagga tgaatccacc gagaagttct
     6241 ataacggtga ttgcgcgatg accaccgcct cttccggttc tctcgctaat attcgtgagt
     6301 acgccaaatt taactatggc gtaggcatga tgccttacga cgccgatgcg aaagatgcac
     6361 cgcaaaacgc cattatcggc ggggccagcc tgtgggtgat gcagggcaaa aataaagaaa
     6421 cgtataccgg cgtggcgaag ttcctcgact tcctcgcgaa gccagaaaac gctgccgagt
     6481 ggcatcagaa aaccggttat ctgccaatca ccaaagcagc gtatgacctg acccgtgagc
     6541 agggctttta tgagaaaaac ccaggggcgg ataccgcgac gcgtcagatg ctgaacaagc
     6601 cgccgttgcc gttcaccaaa gggctgcgtc tgggcaacat gccgcagatc cgcgtgattg
     6661 tggatgaaga gctggagagc gtgtggaccg gtaagaagac accacagcag gcactggata
     6721 ccgccgttga gcgtggaaat cagttgctgc gccgctttga gaaatcgacg aagtcttaat
     6781 cagtgtaatg tcggatgcgt ttcgcttatc tgacctggca tcgcgtgtag gccggataag
     6841 cgaagcgcat ccggcacagt tcaggaatta accgtaatgt catcatcccg tccggtgttc
     6901 cgctcgcgct ggctgcctta tctgctggtc gcgccgcagc tcatcatcac cgttatcttt
     6961 tttatctggc ctgcgggcga agcgttgtgg tactcgctac aaagcgtcga tccgtttggt
     7021 ttctccagcc agtttgtcgg cctggataac ttcgtcgcgc tgtttcatga cagctactat
     7081 ctcgactcct tctggacgac gataaaattc agcacctttg tcaccgtcag cggtttgctg
     7141 gtgtcgctgt tcttcgcggc gctggtggag tacatcgtgc gcggcagccg tttctatcaa
     7201 accttaatgt tgctgcctta cgccgtggct cccgccgttg ccgccgtatt gtggatcttc
     7261 ctgtttaacc ccggtcgcgg gctgatcacc cattttctcg cggagttcgg ctacgactgg
     7321 aaccacgcgc aaaacagcgg tcaggcaatg tttctggtgg tgtttgcctc agtatggaag
     7381 caaatcagct acaacttcct gttcttctat gccgcgctgc aatccattcc ccgttcgttg
     7441 atcgaagccg cagccatcga cggtgcaggg ccgattcgcc gcttctttaa gattgcgctg
     7501 ccgcttatcg ccccggtgag tttcttcctg ctggtagtga acctggtgta tgccttcttc
     7561 gacaccttcc cggtgatcga cgccgccacg tccggcgggc cggttcaggc caccacgacg
     7621 ctgatttata agatctaccg cgaaggtttt accggactgg atctggcttc gtctgccgca
     7681 cagtcggtgg tgttgatgtt cctcgtcatc gtgctgacgg tggtgcagtt ccgctatgtt
     7741 gaaagcaagg tgcgttacca atgattgaga accgtccgtg gctgacgata ttcagccata
     7801 ccatgctgat cctcgggatc gcggtgatcc tcttcccgct gtacgtggcg tttgtcgcgg
     7861 cgacgctgga taaacaggcc gtctatgccg cgccgatgac gctcatcccc ggcacacatc
     7921 tgctggaaaa catccacaac atctgggtga acggggtagg cacgaatagc gcgccgttct
     7981 ggcggatgtt gcttaacagc tttgtgatgg cgttcagcat tacgctcggc aaaattaccg
     8041 tctcgatgct ctcggcattt gccattgtct ggtttcgttt tccgctacgt aacctcttct
     8101 tctggatgat ttttatcacc ctgatgctgc cggttgaagt acgtatcttc ccgacggtgg
     8161 aagtcatcgc caacctgcag atgctcgaca gctacgccgg tttaacgctg ccgctgatgg
     8221 cctcggcgac cgctactttc ctgttccgcc agttctttat gacgctgccg gatgagctgg
     8281 tggaagccgc gcggatcgac ggcgcatcgc caatgcgctt cttttgcgac atcgtttttc
     8341 cgctctccaa aactaatctg gcggcgctgt ttgtgatcac ctttatctac ggctggaatc
     8401 agtatttgtg gccgttgttg attattaccg acgttgatct cggcaccacc gtggcaggga
     8461 tcaaagggat gatcgccact ggcgaaggca ccacggaatg gaactcggta atggcggcga
     8521 tgttgttaac gcttatccct ccggtggtga ttgttttagt gatgcagcgt gccttcgtgc
     8581 gcggcctggt cgatagtgag aaataagatg gcaggactga aattacaggc agtaaccaaa
     8641 agctgggatg gcaaaaccca ggtgattaaa ccactgaccc ttgatgtggc ggatggcgaa
     8701 tttatcgtga tggtcgggcc gtctggctgc gggaaatcga cgctgctgcg catggttgcc
     8761 gggctggagc gggtgacaga aggcgatatc tggatcaacg accagcgcgt gactgaaatg
     8821 gagccaaaag atcgcgggat tgcgatggtg ttccagaatt acgcgcttta tccgcatatg
     8881 agcgtagagg agaacatggc gtgggggctg aaaattcgcg gcatgggcaa gcagcaaatt
     8941 gccgagcgcg ttaaagaagc ggcgcgcatt ctggaactgg acggtctgct taagcgtcgc
     9001 ccgcgtgagc tatccggcgg tcagcgccag cgtgtggcga tgggccgcgc gattgtgcgc
     9061 gatccggcgg tgttcctgtt tgatgagccg ctctctaacc tcgatgccaa gctgcgcgtg
     9121 cagatgcgtc ttgaactgca acagctgcac cgtcgcctga aaacgacttc actctacgtt
     9181 actcacgatc aggttgaagc gatgacgctc gctcagcgag taatggtgat gaacggcggc
     9241 gttgccgaac agattggcac accagttgaa gtctacgaaa agcccgccag cctgtttgtg
     9301 gcgagtttta tcggcagtcc ggcgatgaac ctgctgacag gccgcgtgaa taatgaaggc
     9361 acgcatttcg aactggacgg cggtatcgtg ctaccgctaa acggtggcta ccgtcagtat
     9421 gccgggcgta aaatgactct cggcattcgc ccggaacata tagcgctaag ctcgcaggca
     9481 gaaggtggtg tgccgctggt gatggacacg ctggagatcc tcggcgcaga taacctggcg
     9541 cacggacgct ggggcgagca gaagctggtg gtgcgactgg cgcatcagga gcgcccgacg
     9601 gcaggcagca cgctgtggct gcatctggcg gaaaatcagc tgcatctttt tgatggtgaa
     9661 acaggacaac gagtatgagt aactggcctt atccccgcat cgtcgctcat cgtggcggcg
     9721 gtaagctggc cccggaaaac accctggcgg caatcgacgt cggggcaaaa tacggtcata
     9781 agatgatcga atttgacgcg aagttatcga aagatggcga gatcttcctg ctccatgacg
     9841 acaatctcga acgcaccagc aacggctggg gcgtcgcggg tgaactgaac tggcaggatt
     9901 tactgcgcgt ggatgcgggc agttggtaca gcaaagcgtt taaaggtgag ccgctgccgt
     9961 tgctttcgca ggtggcggaa cgctgtcgcg aacacgggat gatggcgaat atcgaaatca
    10021 aacccaccac tggcactgga ccattaacgg gcaaaatggt ggcgctggcg gcacgccaac
    10081 tgtgggccgg tatgacgccg ccgctgctgt catcgtttga gattgatgct ttagaagccg
    10141 cacaattggc ggcaccggaa ctgccgcgtg gtttattgct ggatgagtgg cgcgacgact
    10201 ggcgcgaact gaccatgcgg ctgggctgcg tctctcttca tctcaatcat aagttgctcg
    10261 ataaagcgcg agtgatgcag ttgaaagacg ccggactgcg gattctggtg tataccgtca
    10321 acaaacccca gcgcgcggca gagttgctgc gctggggcgt ggattgcatc tgtaccgatg
    10381 cgattgatgt aattggtccg aactttacgg cccaatagtt ttcaacggaa tgtcaggctg
    10441 cggcgtgctg ggctgattca gcatgtcgcc gtttctcctc tccggcagca tatgctgctg
    10501 attcaacgaa ctatccggat tacggttgct gtttaacatc ccgccgttgg tgttgggcag
    10561 catttgctgc cgggcgggat ttcgctcccc cggctgtgac tgcaacaccc gctgagaatt
    10621 attgtttatc tggttttcta aatgctgctg ttgcaactga gtttgcgttt tcagttgctg
    10681 attcagcatc cctttttgct ggatttgctg cgtttgcatt tgggtttgca tccgctgctg
    10741 actggggatc tgataccccg gctggttagg gttattcaga gtattaatgg gctgtgcaaa
    10801 gccggcaaac ggcaggagtg ccgtaagaat cagaagtcgt ttcatcgcgt atcctcctct
    10861 gaagatattc tttaagttta ctcgcttccc ggcaaaacga tgattaattc agagttatat
    10921 accaggctta gctggggttg ccccacacat aactggagaa taacgatgat aaaaccgacg
    10981 tttttacgcc gggtggccat tgctgctctg ctctcaggaa gttgttttag cgccgccgcc
    11041 gcgcctcctg tttcttatgg cgtggaggaa gatgtctttc atccggtacg cgcgaaacag
    11101 gggatggtgg cgtctgtaga tgctactgcc actcaggtgg gggtggatat cctcaaggag
    11161 ggcgggaatg ccgttgatgc tgccgtggcg gtgggctacg cgctggcggt aacgcatccg
    11221 caggcgggga atctgggcgg cggtggtttt atgctgatcc gcactaaaga tggcaacacc
    11281 actgctattg atttccgtga gatggcaccc gctaaagcga cccgcgatat gttccttgat
    11341 gatcaaggca acccggacag caaaaaatca ctcacttcgc atctggcctc tggcacgccc
    11401 ggtacggtag caggtttctc gctggcgttg gataaatacg gcaccatgcc gctgaataaa
    11461 gtcgtgcagc ccgcttttaa acttgcacgc gatggtttta tcgttaacga cgcgctggct
    11521 gacgatctca aaacctacgg tagcgaagtg ttgccgaatc acgaaaacag caaagctatc
    11581 ttctggaaag agggcgaacc gctgaaaaag ggcgacaagc tggtgcaggc gaatctggca
    11641 aagagcctgg agatgattgc cgaaaacggc ccggacgaat tctacaaagg cacgatagcg
    11701 gaacagattg cccaggagat gcagaaaaac ggtggcttga tcaccaaaga agatttagcg
    11761 gcgtataaag cggtcgaacg cacaccgata agcggcgatt atcgcgggta tcaggtttac
    11821 tccatgccac cgccatcttc cggcgggatc catatcgtac aaatcctcaa tattttggaa
    11881 aacttcgata tgaagaaata cggctttggc agcgccgatg cgatgcaaat catggcagaa
    11941 gcggagaaat atgcctacgc cgaccgctcg gaatatcttg gcgacccgga ttttgtcaaa
    12001 gtcccgtggc aggcactgac caataaagcc tatgccaaat ccattgccga tcaaattgat
    12061 atcaacaaag cgaagccgtc cagcgaaatc cgtcccggca agcttgcgcc ttatgagagt
    12121 aatcaaacta cccattattc ggtggtggat aaagacggta acgcggtggc ggtgacctat
    12181 acgctgaaca ccaccttcgg tacgggtatt gtcgcgggcg agagcggtat tctgcttaat
    12241 aaccagatgg atgatttctc cgccaaaccg ggcgtgccga acgtttacgg gctggtgggc
    12301 ggtgatgcca acgccgtcgg gccgaataaa cgtccactgt cgtcgatgtc gccgactatt
    12361 gtagtgaaag acggtaaaac ctggctggtt accggaagcc caggtgggag tcggattatc
    12421 accactgtgc tgcaaatggt ggtgaacagc atcgattatg gcatgaacgt cgccgaagcg
    12481 accaatgcac cgcgtttcca ccatcagtgg ttgccggacg agctgcgtgt cgaaaaaggg
    12541 tttagcccgg atacgctcaa gctgctggaa gcaaaaggtc agaaagtggc gctgaaagag
    12601 gcgatgggca gcacacaaag tattatggtt gggcctgacg gtgagttgta cggtgcatct
    12661 gacccgcgct cggtggatga tttaacggcg gggtactaag gttagcggcc ctcttcgtgg
    12721 gaagagggct attttgtcag ggcaagccga aggtagcctt ttttatttcg taatcctgta
    12781 gatattcttc cagcggcttt gccgttccca gagaatgaat ttcaccactg tctttatcaa
    12841 taataaaagg tgcgttacca gctaagcgcg cggcctcatc tccagtttcg agaaattctc
    12901 gtgcttcgaa acagaaatac cagccctggc taaagcgtcc atgtagagta atgacgaccg
    12961 ggagatctgc atcatcaagg taatggttcg ctttcgcgaa tgcgtcgtga taagtaatca
    13021 taattataat aaatattatt gttgagtgtt atattattat tcctgtgtga tacattgagc
    13081 aaagacgcgt tcatcttcgt caaagatatt attaaatgct ggtttt
//
LOCUS       DAZGSP010000135        13031 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZGSP010000135 DAZGSP010000000
VERSION     DAZGSP010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.136"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..512
                     /locus_tag="ACHK74_004444"
     CDS             <1..512
                     /locus_tag="ACHK74_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFP0223149.1"
                     /translation="NGDDFRFLHPDFNYIYQNYGDDFVTHTAKFSGETVERAIERAIV
                     SKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMIKTTSAALSWESTNERYSKDKEAG
                     NIARKVDKNHHDIVTGLLAENARKVFASNLSDKFAVYSREKMIFSSQAATNDDIATLI
                     QNEISGNTQ"
     gene            complement(548..1339)
                     /locus_tag="ACHK74_004445"
     CDS             complement(548..1339)
                     /locus_tag="ACHK74_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFP0223150.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVIYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(1345..2472)
                     /locus_tag="ACHK74_004446"
     CDS             complement(1345..2472)
                     /locus_tag="ACHK74_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HFP0223151.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILLEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
     gene            2978..3961
                     /locus_tag="ACHK74_004447"
     CDS             2978..3961
                     /locus_tag="ACHK74_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFP0223152.1"
                     /translation="MPEKYSPDDQSSTIDPYLITSVRQTLEEQGAALQNLSKQLDSGQ
                     YQCVLNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMIT
                     PCDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            4033..5181
                     /locus_tag="ACHK74_004448"
     CDS             4033..5181
                     /locus_tag="ACHK74_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFP0223153.1"
                     /translation="MLIKVKSAVSWMRAHLSAISLADIQKHLVKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            5205..6881
                     /locus_tag="ACHK74_004449"
     CDS             5205..6881
                     /locus_tag="ACHK74_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HFP0223154.1"
                     /translation="MKLFKSILLIAACHAAQASAAIDINADPNLTGAALLTGILNGQQ
                     SDTQNMSGFDNTQPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNTLVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYASTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLKKVRPNNMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            6891..7631
                     /gene="kdsB"
                     /locus_tag="ACHK74_004450"
     CDS             6891..7631
                     /gene="kdsB"
                     /locus_tag="ACHK74_004450"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFP0223155.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDQRVEKAVQAFGGKAIMTRKDHESGTDRLVEVMHTVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAEEATEPSTVKVVVNTRQDALYFSRSPIP
                     YPRNAEKVRYLKHVGIYAYRRDVLQNYSQLPESMQEQAESLEQLRLMSAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            7628..9655
                     /locus_tag="ACHK74_004451"
     CDS             7628..9655
                     /locus_tag="ACHK74_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFP0223156.1"
                     /translation="MIGIYSPGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLGVNGEPPLSLVVDDCGIYYDASKPSAL
                     EKLVQDKAGNTALISQAREAMHTIVTGDLSKYNLAPAFVADESERSDIVLVVDQTFND
                     MSVTYGNAGPHEFAAMLEAAMAENPQAEIWVKVHPDVLEGKKTGYFADLRATQRVRLI
                     AENVSPQSLLRHVSQVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSATLEELFAAAYLRYCRYIDPQTGAVSALFTVLQWLQLQRRHLQQRNGYLWAP
                     GLTLWKSAILKPFLRTATNRLSFSRRCTAASACVVWGVKGEQQWRAEAQRKSLPLWRM
                     EDGFLRSSGLGSDLLLPLSLVLDKRGIYYDATRPSDLEVLINHSQLTLAQQMRAEKLR
                     QRVVESKLSKYNLGADFSLPAEAQDKKIILVPGQVEDDASIKTGTVSIKSTLELLRTV
                     RERNPHAYIIYKPHPDVLVGNRKGNIPTELIAELADYQALDADIIQCIQHADEVHTMT
                     SLSGFEALLHGKQVHCYGLPFYAGWGLTVDEHHCPRREQKLTLADLIYQALIVYPTYI
                     HPTRLQPITVEEAVEYLIQTPRKPLFITRKKAGRVIRYYRKLIMFCKVRFG"
     gene            9690..10889
                     /locus_tag="ACHK74_004452"
     CDS             9690..10889
                     /locus_tag="ACHK74_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFP0223157.1"
                     /translation="MQGNALTILLSGKKYLLLQGPMGPFFSDVAEWLESLGRNAVNVV
                     FNGGDCFYCRHRHYQAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKYWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLSRDPDFYRKLPDMPAPHVENLKPSTM
                     KRIGHAMWYYLMGWHYRHEFPLYRHHKSFSLWYEARCWVRAYWRKQLYKVTQRKVLPR
                     LMNELDQLYYLAVLQVYNDSQIRNHSNYNDVRDYINEVIYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLGGRVIYVHDLPMPELLRHAKAVVTINSTVGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDMKLFKKFRGYILMKTQLNAVYYGIF
                     NFRKITN"
     gene            11707..12483
                     /locus_tag="ACHK74_004453"
     CDS             11707..12483
                     /locus_tag="ACHK74_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0223158.1"
                     /translation="MKRSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPAAHLL
                     VLLGIFGYIMHRTMPGISFPVFLLNGLIPFFIFNSISNRSVEAIDANQGLFNYRPVKP
                     MDTIIARTLLETLIYIIVYVLLMLIVWMSGEYFEIISLLQLVLVWTLLIIISCSVGLI
                     FMVVGKTFPEMQKILPIFLKPLYFISCIMFPLHSIPQQYWTYLLWNPLVHVVELSREA
                     VMPGYISEGVSLNYLAMFTLVTLFIGLALYRTREEAMLTS"
     gene            12480..>13031
                     /locus_tag="ACHK74_004454"
     CDS             12480..>13031
                     /locus_tag="ACHK74_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0223159.1"
                     /translation="MIKIENMTKSYRTPTGRHYVFKDLNIELPSGKSVAFIGRNGAGK
                     STLLRMIGGIDRPDSGKIITNKTISWPVGLAGGFQGSLTGRENVKFVARLYAKPEELK
                     EKIEFVEEFAELGKYFDMPIKTYSSGMRSRLGFGLSMAFKFDYYIVDEVTAVGDARFK
                     EKCAHYFKERHKESSFLMVSHSLN"
BASE COUNT         3457 a         3034 c         3150 g         3390 t
ORIGIN      
        1 ttaacggtga tgattttcga tttctacacc ctgattttaa ttatatctac caaaattatg
       61 gggatgattt tgttacccat acagcgaaat tttccggcga aacggtcgaa cgtgcaattg
      121 agagagcgat agtcagtaaa ctcaacattg ttgtcgaagg aacatttcgt aatgccgcga
      181 cgccgttaca aacattaaaa aaactcaaag atgcaggcta ccaaacagaa gtcatgataa
      241 aaacaacctc agcggcatta agctgggaaa gcaccaatga acggtatagt aaggacaaag
      301 aggcgggaaa tattgcccga aaagtggata aaaaccacca tgatatcgtc accggtttgc
      361 tggcagaaaa tgcccgcaag gtgtttgctt cgaacctgtc agataagttt gcggtgtatt
      421 cgcgtgagaa gatgatattt agttcacaag cggcaacgaa tgacgatatt gcaacactta
      481 tacagaatga aatcagcggg aatacacaat aataataaga gctgatggcg tgaaataacg
      541 ccgggcttta tgcccggcgc attaccgtct ggtacattaa gctatccagt tccttaacgt
      601 ccccttctct gctatccggt aacagcggtg gaatttcccc acgcgccagt tccgcacgaa
      661 taaacgcatg taacaatgga cggcgcggac catattccgc ttctcctgcg cgctgtttac
      721 gttccagtaa ttcgtctatt tctgcacgta acggcgcatc aagttcgctt cccgccagta
      781 actcagcaaa gcgcattggc ggtacgcctt tacccgcttc gacccagcgc acggccagca
      841 gcggacgcag cacgtaaaag tattttttca gtcgaacttc gtcgccctgc aaatatccgg
      901 ggaagttttt ctgcgccatc gaatagtaat gccagcgcgc acgcagcgga gaaaaccaca
      961 ttgggaccat tgccttaaac gccgttatcg tttcttcatc ttgttgataa atcaccggag
     1021 aatccagcca ttctatcagc gtcgggttcg cgcctttcaa taatcccagc gccttacgcc
     1081 actcccaacc ggaaacatct aactcgtcgt cgatgggcag ctcaataaca tcgcgcggtg
     1141 actctactcg cagataccat tccagcgggt gaacatacaa aaaccgtaca tcgtagtcgc
     1201 tgtcgggcga ggcaaatccc cagccgcggc tccctgattc acaggcgtac agtactttca
     1261 catcataccg ccgttcaatt tcttttaact gctgcgatac cctttcgcgc atcgcagcac
     1321 tgactccatt taatggcatg ttactcaccc tttgatatta accaacccat gcagaacctg
     1381 atccagaccg ccaaatacag agatgttatc gatccgctca gccactctct ccagcgtttc
     1441 tagctctttt aaacgcaacg ctaccggatt gttctccatg actttggcgg tgttcagtaa
     1501 cgagcgcgtc gctgccgttt cttcacgtcg gcgaatcaca ttcgcctgcg cagatttctc
     1561 tgcctcaacc agctgcgcga ggatattttt catatcacca ggcaggacga tatctttcac
     1621 ccctaatgag gcaatctcca taccaaatgg caacatgcgc gatttcacct gttcgctgac
     1681 aacgtcatca atgacctgtt tatcttccag cagttcatcc agcgtacgag tacctaccgc
     1741 ttcacgcagg gcaaattgca attcccgata caaatgatca atcggcttcg ttaactgact
     1801 aaatgccagc aacacgtcgc tgtagcgcca gttagcggcg aggttaatcc gcagattgac
     1861 cttatctttc gttaagattt cttgcccgct gacttccagt acctgcaagc gggtgtcaac
     1921 cacttcagcg tcaaccagat ggttaatttt ccagtatgcg gtcagccccg gcggtaacag
     1981 cgcctgcgtt tcaccatcga ttttcagcac gcctacatgc caggccggaa cctgcaccgt
     2041 tagtattgca tcgcggcctt taactgcccc tttgcgccgt ggttgcaata ccgcattcat
     2101 cacctccgtt tgtacctgga cttgctgcgt attcatccgc accagtgtca aatcatcttc
     2161 tacacgccag tacaaacggc gcgttgatgg cggcagaatc tccagcaaaa taccgtccat
     2221 atacaacgcc ccggcttcaa tctccgacaa gtctgccacc aggcaatatt tctccaccca
     2281 atcaggctga aagcggcgca aataatccgc cagcacgtcg ggaacttcgc tgccatcgag
     2341 cgtaatcagc aagacttctg gcgtatttaa ccagggtaaa agatgctcgc ctgcatgtag
     2401 cactttgtag taatcgccgt ttctggagag taatgccagc tgatcttttc ttacgcttat
     2461 ttttttaatc attatctatt ccttgcttaa cagccgtcgc ggaagcagac acgaagcgac
     2521 aggggaattc ccttttgcct gttacccggc atctttatcg gcgacgttat cggggatcgc
     2581 tgactgcgca atccctcact ttttatttaa taagttacaa ccagtattca tttcaggaat
     2641 gaatgtggga atcgaaccca taattggcgt caaaggctga cggaacacgc ccggaaaaca
     2701 gcctggccgt tcctggtgtg ccgacggggt ttgaccccct ttgtaattca gtttcaacgc
     2761 gtacgagata ttgcgagaag cgtgccagaa ttgagaaaaa attattaacc aaatgaaaaa
     2821 taaagattta ataaattatt tttgtttgta gactgtgtta tatgctttat ggaaaagtag
     2881 tattttgatt tatgtgaaga tgagctgaac tcgatttatc agaggcaagt tgtctttatg
     2941 ttataagtgt tttaacttta tcggaagtat aatttatatg ccagaaaaat attcacctga
     3001 tgatcagagc agtactattg atccatattt aattacttct gttcgtcaga ccttggaaga
     3061 acaaggtgca gcattacaaa acttgtctaa acaactggat tccgggcagt accagtgtgt
     3121 ccttaatttg ataatgaact gtaaagggca cgttattctt tcgggaatgg gtaaatcagg
     3181 gcatgtcggt cgtaaaatgt cagcgacgct ggcctctacc ggtaccccga gtttctttat
     3241 tcatcctgca gaagcgttcc atggcgatct gggtatgatt acgccctgcg atcttttgat
     3301 ccttatttct gccagcggtg aaacggatga aatcctcaag ctagttcctt cactgaaaaa
     3361 tttcggcaac cgaattatcg ccattaccaa taatggaaat tccacgctgg cgaaaaatgc
     3421 tgatgccgtg ctggaactcc atatggcgaa tgaaacctgc ccgaataatc ttgcaccaac
     3481 aacgtctacc accctgacca tggcgatcgg cgatgcgctg gcgattgcca tgatccacca
     3541 acgcaaattt atgccgaatg attttgcgcg ctatcacccg ggcggttcat taggtcgtcg
     3601 cctgctgacc cgcgttgctg atgtcatgca gcatgatgtt cctgcggtac agctggatgc
     3661 gtcatttaaa accgtgattc aacgtatcac cagcggatgc cagggaatgg tgatggtaga
     3721 agacgcagaa ggtgggctgg cgggcattat aaccgacggt gacctgcgtc gctttatgga
     3781 aaaagaggat tctctgacat ccgctacggc tgcgcagatg atgacacgtg aaccgctgac
     3841 gctaccggaa gacactatga tcattgaagc ggaagaaaaa atgcaaaagc accgcgtctc
     3901 aacgttattg gtgaccaaca aggcaaataa agtcactggc cttgtgcgca ttttcgacta
     3961 attaagcaac ggggtttgag aggttagtct ctcattgagt ttgcgcccca ctgaaacttt
     4021 gataatcgtt acatgttgat aaaagtgaag tctgccgtat cctggatgcg tgctcatctg
     4081 tctgccatct cactggcaga tatccaaaaa cacctggtga aaatcatcat tctggcaccg
     4141 atggcggtgc tgctgatcta tctggctatc ttcagccagc ctcgctatat gagcgagtcg
     4201 aaagtcgcca ttaaacgctc ggatgattta aacagcggca gcctgaattt tggtctgctt
     4261 ctgggtgcct ctaaccccag ttccgcagaa gatgcgttgt atctgaaaga gtacatcaac
     4321 tcgccggata tgctggcggc gctggataaa caactaaatt ttcgtgaagc gttcagccac
     4381 agcgggctcg attttcttaa tcatcttagc aaggatgaaa ccgcagaagg cttcctgaag
     4441 tactacaagg accgtatcaa cgtctcgtat gacgataaaa ccggattact gaatattcag
     4501 acgcagggct ttagcccgga gtttgcgctt aagtttaacc agaccgtgct gaaagagtca
     4561 gagcgcttta tcaatgagat gtcacatcgc atcgcgcgtg accagcttgc ctttgcagaa
     4621 acggagatgg aaaaggcacg ccagcgtctg gacgccagca aagcggaatt gctctcttat
     4681 caggacaaca acaacgttct ggatccacag gcacaggcac aggcggcgag cacgttagtc
     4741 aatacgctga tgggccagaa gatccagatg gaagcggatc tgcgtaactt gctgacgtat
     4801 ctgcgtgagg acgccccgca agtggtgagt gcgcgtaatg cgattcagtc attacaggca
     4861 caaatcgatg aagaaaaaag caaaatcact gcaccacagg gtgacaagct aaaccgtatg
     4921 gcggtggatt ttgaagaaat caaatcaaaa gttgagttca acaccgagct ctacaaactg
     4981 accctgacct ccattgaaaa gacccgtgta gaagcggctc gtaagctcaa ggtgctgtca
     5041 gtgatcagtt cgccacagtt gccgcaggaa tcgtcttttc caaatatccc ttatttgatc
     5101 gcctgctggt tactggtgtg ctgcctgctg ttcggcaccc tgaaactgtt gctggctgtc
     5161 attgaagatc accgagacta acgctgtcgc tgaatgagtt tgtgatgaaa ttatttaaat
     5221 caattttact gattgccgcc tgtcacgcgg cgcaggccag cgcggccatt gatattaacg
     5281 ctgacccaaa ccttacagga gccgcgctgc ttaccggtat tctgaacggg caacagtcgg
     5341 atacgcaaaa catgagcggc ttcgacaata cccagccgcc cgcaccgccg gtggtaatga
     5401 gccgcatgtt tggtgctcaa cttttcaacg gcaccagcgc ggatagcggt gcgacggtag
     5461 gattcaaccc tgactatatt ctgaatccgg gtgatagcat tcaggttcgc ttgtggggtg
     5521 cgttcacctt tgatggtgcg ttacaggttg atcccaaagg taatattttc ctgccgaacg
     5581 ttggtccggt gaaagttgct ggcgtcagta atagtcagtt aaataccctg gtcacatcca
     5641 aagtgaagga agtgtaccag tccaacgtca acgtatacgc ctccttatta caggcgcagc
     5701 cagtaaaagt gtacgtgacc ggatttgtgc gtaatccagg tctgtatggc ggagtgacgt
     5761 ctgattcgtt actcaattat ctgatcaagg ctggcggcgt tgatccagag cgcggaagtt
     5821 acgttgatat tgtggtcaag cgcggtaacc gcgtgcgctc caacgtcaac ctgtacgact
     5881 tcctgctgaa cggcaaactg gggctttcgc agttcgctga tggtgacacc atcatcgtcg
     5941 ggccgcgtca gcatactttc agcgttcagg gcgatgtctt taacagctac gactttgagt
     6001 tccgcgaaag cagcattccc gtaacggaag cattgagctg ggcgcgccct aagcctggcg
     6061 cgactcacat tacgattatg cgtaaacagg ggctgcaaaa acgcagcgaa tactatccga
     6121 tcagctctgc gccaggccgt atgttgcaga acggcgatac cttaatagtg agcactgacc
     6181 gctatgccag caccattcag gtacgggttg aaggtgcgca ctccggtgaa catgccatgg
     6241 tactgcctta tggttccact atgcgtgcgg ttctgaaaaa agtccgcccg aacaacatgt
     6301 cgcagatgaa cgcggttcag ctttatcgcc catcagtggc tcagcgtcag aaagagatgc
     6361 tgaatctctc gctgcaaaag ctggaggaag cgtcactttc tgcccagtca tccactaaag
     6421 aagaagccag cctgcgaatg caggaagcgc aactgatcag ccgctttgtg gcgaaagcac
     6481 gcactgtggt tccgaaaggt gaagtgatcc tcaatgaatc caatattgat tctgttctgc
     6541 ttgaagatgg cgacgtcatc aatattccgg agaaaacatc gctggttatg gttcatggtg
     6601 aggtgctgtt cccgaacgca gtgagctggc agaagggtat gaccaccgag gattacatcg
     6661 agaaatgtgg tggcctgacg cagaaatcgg gtaacgccag aattatcgtc attcgtcaga
     6721 acggtgctgc cgtcaacgca gaagatgtgg attcactcaa accgggcgat gaaattatgg
     6781 ttctgccgaa atatgaatcg aaaaatattg aagttacccg tggtatttcc accatcctct
     6841 atcagctggc ggtgggtgca aaagtgattc tgtctttgta aggagttgaa atgagcaaag
     6901 ctgttattgt catcccggct cgttatggct cctcgcgcct gccgggtaag ccactgctcg
     6961 atattgttgg taaaccgatg atccagcatg tttacgagcg tgcgttacag gtggcgggcg
     7021 ttgcggaagt ttgggtggca acagacgatc agcgtgttga aaaggccgtg caggcatttg
     7081 gcgggaaagc catcatgacg cgcaaagatc atgaatccgg caccgatcgg ctggtcgagg
     7141 tgatgcatac ggtggaagca gatatctaca ttaacctgca gggcgacgaa ccaatgattc
     7201 gcccgcggga tgtagaaacg ctgttacaag gaatgcgaga cgatcccgcg ttgccagtgg
     7261 caacgctatg ccacgctatt tctgccgaag aagcgaccga gccaagcacg gtaaaagtgg
     7321 ttgtgaatac ccgccaggat gcgctttatt tcagccgttc gccgattccg tatccgcgta
     7381 atgctgaaaa agtgcgctac ctgaaacacg ttggtattta cgcttatcgt cgcgatgtgc
     7441 tgcaaaacta cagccagtta ccggagtcca tgcaggagca ggcagaatca ctggagcagc
     7501 tgcggttgat gagcgcgggg atcaacatcc gtacatttga ggttgctgca accggtccgg
     7561 gcgtcgacac cccagcatgc ctggaaaaag tgcgcgccct gatggcacag gaactggctg
     7621 aaaacgcatg attggcattt actcgcctgg catctggcgt attccgcatc tggagaaatt
     7681 tctggcgcaa ccgtgccaga aactttctct gctgcgccct gttccgcaag aagttgatgc
     7741 tatcgccgtg tggggacatc gtcccagcgc ggcgaaacca gtcgccatcg ccaaagcagc
     7801 gggaaaaccc gtcattcgtc tggaagatgg atttgtgcgt tcgctggatc ttggcgtcaa
     7861 tggcgagccg ccgctttctc tggtggtgga tgattgtggc atttactacg atgccagcaa
     7921 gccttcagca ctggagaaac tggtacagga taaagccgga aatacagctc tgataagcca
     7981 ggccagagaa gcgatgcaca ccatcgtgac cggggatttg tcgaaatata acctggcacc
     8041 tgcgtttgtg gctgatgagt cagaacgttc agacatcgtt ctggttgtcg atcagacatt
     8101 taatgatatg tcagtgacgt atggcaatgc tggcccgcat gagtttgctg ccatgctgga
     8161 agccgcgatg gcggaaaatc ctcaagccga aatttgggtg aaggtgcatc cggatgtcct
     8221 ggaaggaaag aaaacaggtt atttcgctga tctgcgcgcc acgcaacgag tacgtttgat
     8281 tgccgagaat gtcagcccgc agtcgctgtt gcgacacgtt tcccaggttt acgtcgtgac
     8341 atcccaatac ggctttgaag ccttgctggc aggaaaaccg gtgacatgct tcggccagcc
     8401 ctggtatgca ggctggggct taacggacga tcgtcatccg cagtcggcgt tgttatctgc
     8461 ccgacgcggt tcagccacac tggaagaact ttttgccgct gcatacctgc gttactgtcg
     8521 ctatatcgat ccgcaaacgg gagcagtaag cgctctgttt accgtgctgc aatggctgca
     8581 attacaacgc cgacatctgc aacagcgtaa tggctattta tgggcgccag gcttaacgct
     8641 gtggaagtca gcgatcctga aacctttctt gcgaacagcg acaaaccggc tgagtttttc
     8701 acgtcgttgt actgcggcga gcgcctgcgt ggtatggggt gtaaagggag aacagcaatg
     8761 gcgagccgaa gcgcaacgaa aatcactgcc gttatggcga atggaagatg gttttctgcg
     8821 ttcatccgga cttggctctg accttttgct gccgctatcg ttggtgctgg ataaacgcgg
     8881 gatctactat gacgccacgc gccccagcga cctggaagtg ctgattaatc acagccagtt
     8941 aacactggcg cagcagatgc gagctgagaa gttgcggcaa cgggtggttg aaagcaaact
     9001 gagcaaatac aacctggggg ccgatttctc tctgcctgcc gaagcccaag ataaaaaaat
     9061 catcctggtg ccgggtcagg tagaagacga tgcctcaatt aaaacaggca ctgtgtcgat
     9121 taagagcacc cttgagttat tacgcacagt acgcgagcgc aatccgcacg cttacattat
     9181 ttataaaccg cacccggatg tactggtggg gaatcgcaag ggcaatattc cgacagaact
     9241 aattgctgaa ctcgctgatt atcaggcact ggacgcagat attattcaat gcattcagca
     9301 cgcagatgaa gtgcacacca tgacatcatt gtccgggttt gaagcgttat tacatggcaa
     9361 gcaagttcat tgttacggcc tgcccttcta tgccggttgg ggtttaaccg ttgatgaaca
     9421 tcactgcccg cgccgcgaac aaaaattaac gttagcagat ttgatctatc aggcgttgat
     9481 tgtttatcca acctatatcc acccaacacg gctacaacct attacggttg aagaggcggt
     9541 ggaatatttg atccagacgc cgcgcaagcc gctgtttatt accagaaaaa aagcggggcg
     9601 agtaatacgt tattaccgca aattaattat gttctgcaag gtcagatttg gctaaacaat
     9661 ttcatagttg actattacgc aaattattta tgcaaggtaa tgcactaacc attttattat
     9721 ctggtaaaaa atatctgcta ttgcagggac cgatgggacc ctttttcagt gatgtagccg
     9781 agtggctgga gtcattaggc cgtaacgctg tgaatgttgt attcaatgga ggggattgtt
     9841 tttactgtcg ccaccgacat tatcaggctt actaccagac accgaaagag tttcccggtt
     9901 ggttacggga tctccaccgg caatatgact ttgataccat cctctgcttt ggtgactgcc
     9961 gcccattaca caaggaagca aaatattggg caaagtcgaa aggtatccgc tttctggcat
    10021 ttgaggaagg atatttacgc ccgcagttta ttaccgttga agaaggcgga gttaacgcat
    10081 attcatcgct atcgcgcgat ccggattttt atcgtaagtt accagatatg cctgcgccgc
    10141 atgttgagaa cttaaaacct tcaacgatga aacgtatagg ccatgcgatg tggtattatc
    10201 tgatgggctg gcattaccgc catgagttcc ctctctaccg ccaccataaa tcgttttccc
    10261 tctggtatga ggctcgttgc tgggttcgtg cgtactggcg caagcaactt tacaaagtaa
    10321 cacagcgtaa ggtattgccg aggttaatga atgagctgga ccaactttat tatcttgccg
    10381 ttttgcaggt gtataatgat agccagattc gtaaccacag taattataac gatgtacgtg
    10441 actatattaa tgaagtcata tactcatttt cgcgtaaagc tcctaaagag agttatttgg
    10501 tgatcaaaca tcatccgatg gatcgtggtc acagactcta tcgaccatta attaagcggt
    10561 tgagtaagga atatggctta ggtgggcgag tcatttatgt gcacgatctc ccgatgccgg
    10621 aactattacg ccatgcaaaa gcggtggtga caattaacag tacggtgggg atctctgcac
    10681 tgattcataa caaaccactc aaagtgatgg gcaatgccct ctacgacatc aagggcttga
    10741 catatcaagg gcatttacac cagttctggc aggctgattt taaaccagat atgaaactgt
    10801 ttaagaagtt tcgtggatat atattgatga agacacagct taatgcagtt tattatggga
    10861 tttttaattt tagaaaaata acgaattaat aaaacatcta taatacaaac aacggttgtt
    10921 gtatattatt tattgaattc aatcattttt atacttaatg tttttgtaaa taataagttg
    10981 actgtgttgt actatattaa tataggcgtt tatattcata tctttaagag taagctatta
    11041 gatgtaaatt actttggtca cgtccttcac agcagaaaca gaatgtcatg aaagataaat
    11101 ttacgcagtc acttgatcaa ctgcttagtt ttgcgtctta aaggattgta actatggcaa
    11161 ctgtggagcg aaaaatcatt gggcatggga aaagtgcggt catatagaaa agtgctctct
    11221 attcagcatc tcatggttta tcagcgaaga cagaaaaaaa attataacga tgcgcaggaa
    11281 tttgtggagg tttgctaata tagagcgatt cgagcatccc tcgttaaatc tctcgaaccg
    11341 cagtatattc agacattttt aaaatgcgcc agatgattac gacagaaaga acacaacata
    11401 taattcatgt tcgctgtcct cttgctgaat acggaattgt cttaaataaa ggtgctacac
    11461 attatggtga gagctaccac ggtttcctga ggatgcagaa aatggattta ccgatatcgt
    11521 gcaattttgt atcggtagta tgtccgactc atgtctcatg gataatgaac catatattat
    11581 aactgcgggt agaaaaatgc gcatctaacg actttgcctg gacgtgtcga ttaactgttc
    11641 ctgggtttgc tacttttgtc agtcagacga taaaagtgag cataaatata taggattaca
    11701 tatcaagtga aaagaagtgg atttgaagtt cagaaggtga cagtagaggc attatttcta
    11761 cgagaaatac gaacacgttt tggtaagttt cgtctgggat atctgtgggc aatcctagaa
    11821 cccgctgctc atttgctggt gctgttaggt atttttggct atattatgca tcgtacgatg
    11881 ccaggtatct cgttcccggt atttttactt aatggtttga ttcccttttt tatctttaac
    11941 agtatcagta atcggtctgt ggaagctatt gatgcaaacc aggggctttt taattatcgg
    12001 ccagtaaagc ccatggatac aataatagcc agaaccctac ttgagacact aatttacatt
    12061 atagtttatg tgttgcttat gcttattgtc tggatgtcag gagaatattt tgaaataata
    12121 agcttattac aactggtact tgtatggact cttctgatta ttatttcatg tagtgtcgga
    12181 ttaatattta tggtcgttgg taaaactttc cctgaaatgc aaaagattct accgatattt
    12241 cttaaaccgc tgtatttcat ttcctgtatc atgttccctc tacattcgat cccacagcaa
    12301 tactggacat atctactctg gaacccatta gtccatgtcg tagagttaag tcgtgaggca
    12361 gttatgccgg gctatatcag cgaaggcgtg agtctgaact acctggcaat gttcactctg
    12421 gtcaccctgt tcatcggtct ggcgctatac cgaacgcgtg aagaggcaat gctgacatca
    12481 tgattaagat tgagaatatg acgaagtcat atcgcacacc aacaggtcga cactatgtgt
    12541 ttaaggattt aaatatcgaa ctcccttcag gaaaaagtgt tgcctttatt ggtcgtaatg
    12601 gtgcagggaa atcaacttta ctgagaatga ttggcggtat tgaccgcccc gatagcggaa
    12661 agatcatcac caataaaacg atatcatggc ccgttggcct cgccggtgga ttccagggaa
    12721 gtttaaccgg acgcgaaaac gtaaaatttg ttgcgcggtt atacgcaaag ccagaagaac
    12781 tgaaagagaa aattgagttt gtcgaagaat ttgccgaact cggtaagtac tttgatatgc
    12841 cgatcaaaac ttactcatct ggtatgcgat ctcgcctggg ttttggttta agcatggcat
    12901 ttaagtttga ttattatatt gtcgatgaag taacggcagt cggtgatgct aggtttaaag
    12961 aaaaatgtgc acattacttt aaagaaaggc ataaagagtc tagtttttta atggtttctc
    13021 atagtttaaa t
//
LOCUS       DAZGSP010000136        12928 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZGSP010000136 DAZGSP010000000
VERSION     DAZGSP010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.137"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            274..537
                     /gene="ykgM"
                     /locus_tag="ACHK74_004455"
     CDS             274..537
                     /gene="ykgM"
                     /locus_tag="ACHK74_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP0223160.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            537..677
                     /gene="ykgO"
                     /locus_tag="ACHK74_004456"
     CDS             537..677
                     /gene="ykgO"
                     /locus_tag="ACHK74_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFP0223161.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(712..937)
                     /gene="ykgL"
                     /locus_tag="ACHK74_004457"
                     /pseudo
     CDS             complement(712..937)
                     /gene="ykgL"
                     /locus_tag="ACHK74_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
     gene            1760..2302
                     /gene="ecpR"
                     /locus_tag="ACHK74_004458"
                     /gene_synonym="matA"
     CDS             1760..2302
                     /gene="ecpR"
                     /locus_tag="ACHK74_004458"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFP0223162.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            2377..2964
                     /gene="ecpA"
                     /locus_tag="ACHK74_004459"
                     /gene_synonym="matB"
     CDS             2377..2964
                     /gene="ecpA"
                     /locus_tag="ACHK74_004459"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFP0223163.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            3024..3692
                     /gene="ecpB"
                     /locus_tag="ACHK74_004460"
                     /gene_synonym="yagY"
     CDS             3024..3692
                     /gene="ecpB"
                     /locus_tag="ACHK74_004460"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFP0223164.1"
                     /translation="MKKHLLLLALLLSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            3718..6243
                     /gene="ecpC"
                     /locus_tag="ACHK74_004461"
                     /gene_synonym="yagX"
     CDS             3718..6243
                     /gene="ecpC"
                     /locus_tag="ACHK74_004461"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFP0223165.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDHGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGIYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGPFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            6233..7876
                     /gene="ecpD"
                     /locus_tag="ACHK74_004462"
     CDS             6233..7876
                     /gene="ecpD"
                     /locus_tag="ACHK74_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFP0223166.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            7845..8555
                     /gene="ecpE"
                     /locus_tag="ACHK74_004463"
     CDS             7845..8555
                     /gene="ecpE"
                     /locus_tag="ACHK74_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFP0223167.1"
                     /translation="MFRRRGVTLTKALLTAVCMLATPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPECDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            8868..9197
                     /gene="ykgJ"
                     /locus_tag="ACHK74_004464"
     CDS             8868..9197
                     /gene="ykgJ"
                     /locus_tag="ACHK74_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP0223168.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(9445..10059)
                     /gene="yagU"
                     /locus_tag="ACHK74_004465"
     CDS             complement(9445..10059)
                     /gene="yagU"
                     /locus_tag="ACHK74_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFP0223169.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            10477..11166
                     /gene="paoA"
                     /locus_tag="ACHK74_004466"
     CDS             10477..11166
                     /gene="paoA"
                     /locus_tag="ACHK74_004466"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFP0223170.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEIITIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            11163..12119
                     /gene="paoB"
                     /locus_tag="ACHK74_004467"
     CDS             11163..12119
                     /gene="paoB"
                     /locus_tag="ACHK74_004467"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFP0223171.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIHPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDTLFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            12116..>12928
                     /gene="paoC"
                     /locus_tag="ACHK74_004468"
     CDS             12116..>12928
                     /gene="paoC"
                     /locus_tag="ACHK74_004468"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFP0223172.1"
                     /translation="MKFDKPAGENTIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLP"
BASE COUNT         3398 a         3320 c         3224 g         2986 t
ORIGIN      
        1 acacgctgtc aggtaatcaa tcatactgcg ataaatcatc ggccagtaaa gtggagataa
       61 cctccattgt cgaaaaatcc atactctcag cgaaaccatc atcaatcact catccaggcg
      121 tttatgggag cgtcgccaac ggctgctaac aatgccagac ttccccgttg gggaaattcc
      181 acataccaaa aatgggcaca gggattgggt gttgaaatga tccggatgag catgtatctt
      241 tatggttatg ttataacata acaggtaaaa atgatgaagc ccaatatcca tcctgagtat
      301 cgtactgtgg tgttccacga caccagtgtt gatgagtact ttaaaatcgg ctcgactatc
      361 aaaacagacc gtgagattga gctggatggc gtaacgtatc catacgtgac aattgatgtc
      421 tcttcgaaat cgcacccgtt ctatacaggg aagctgagaa cagtggcatc agaaggaaat
      481 gttgcacggt tcacccaacg ttttggtcgt tttgttagca cgaaaaaggg ggcatgatga
      541 aagtccttaa ctctctgcgt accgcaaaag aacgccatcc agactgtcag attgtgaagc
      601 gaaaaggacg gctgtatgtg atttgtaaat ctaatccacg ttttaaggcc gttcagggtc
      661 gtaagaaaaa acgttgattt aaaattcgac gaattaacga tatttgtctg attaataatc
      721 agaccggatt aatgttggtg tatttattac accagcatta atttttctgg ggatgtatct
      781 tcctgttcat ttaaggtcaa ctgcctgacg tttctctccg aatattccat tatattaatg
      841 ttgacttgtt gaccagcttc gccctgtatg ctggcatcaa ccctctttta gactgaacac
      901 gccactcagt ttcctccctt tgcgacgcag cctgcattta cactcaaact gttaagatga
      961 taaatgtggt aaatctgttg gtactgacat aaaaacgttt acgccacagg aacggcctga
     1021 tccaccggta accccatcgc cgacgttcga gtgccagtta gagtaacgcg cacagatgac
     1081 tgaatgcagt gccctggcaa agaggccatc gttcctgtga caactggcag tcttcgttta
     1141 acttcactta atttggctct tagggggctt accggacaga tgacgtactt atacctgttt
     1201 aatttttcat cacttattgg gatgaacacc cataaccatt ttgtgcggca tgttaatcca
     1261 ttaaaacacc ttactgattg gcaaatcatc tttaattatg actcatgata gttttatatt
     1321 ctatttctta tcatttaaac ttgtttttta ctagtccatt acacaacaca ttaagactat
     1381 tcctaacacc tcagggcaaa gttcctggct aatataaaat gcaagtaaga attgaacgtt
     1441 atattgccaa taaccttatg aaactgaatg tctttttctt cttatcaaaa aagcaatatt
     1501 ttcagttttt ctaaatattg acttaaccat tgaattcctt ttctgttcac atattgacac
     1561 tcatcaggaa aaaaacataa atttaaacct aatcgaaata attaaaactt aatctcgttt
     1621 aacctatatt gatatgtgct acgtatctta tttacttccg atttactaaa gaaactgaat
     1681 gtacctgtaa aaattacagg tttggaaagt agtgacatgg caaaatgatt acagcaggga
     1741 ctatgaggtt aaaaaccata tggaatgtca aaaccgttct gataaataca tctggtctcc
     1801 ccatgacgcc tacttctata aaggactatc tgaactgatt gtggatatcg acagattaat
     1861 ttatctatcg ctggagaaaa tcagaaaaga tttcgtgttt atcaatctca atacggattc
     1921 tttaactgaa tttataaacc gtgataatga gtggttatcc gcggtaaagg ggaaacaggt
     1981 cgtattgatt gcggccagaa agtcagaagc cttagcaaat tattggtatt acaacagcaa
     2041 tattaggggc gtggtatacg ctggactgag tcgtgatatt agaaaagaac tggcctatgt
     2101 gattaatggc aggttcctga gaaaagatat taagaaagat aaaatcactg accgagaaat
     2161 ggaaattatc cgcatgacgg ctcagggaat gctgcctaaa tcgattgcca gaattgaaaa
     2221 ttgtagtgta aagacagtgt atacccatcg gcgtaatgca gaggccaagc tgtactcaaa
     2281 aatatataag ttggttcagt aaactccagg caagttagtt ttaaaaaatg actcactggg
     2341 acatcacgtc ctcaattcaa ctcgggaaga aatgcaatga aaaaaaaggt tctggcaata
     2401 gctctggtaa cggtgtttac cggcatgggt gtggcgcagg ctgctgacgt aacagctcag
     2461 gctgtagcga cctggtcggc aacagccaaa aaagacacca ccagtaagct ggttgtgacg
     2521 ccactcggta gcctggcgtt ccagtatgcc gaaggcatta aaggttttaa ctcacagaaa
     2581 ggtctatttg acgtggctat cgagggtgac tcaacggcta ccgcctttaa actgacctca
     2641 cgtcttatca ccaacacctt aacccagttg gatacctcag gttctacact gaatgtgggc
     2701 gtggattata acggcgcggc agtcgaaaaa actggcgata ccgtgatgat cgataccgcc
     2761 aacggcgtac tgggcggcaa ccttagcccg ctggctaacg gttacaatgc cagcaatcgt
     2821 accaccgcac aggatggttt cactttctcc atcatcagcg gcaccaccaa tggtaccacc
     2881 gcagtaaccg attacagcac tctaccggaa ggcatctgga gcggcgacgt tagcgtacag
     2941 ttcgacgcga cctggaccag ttaatctctc tgatgtacca gcaggggtag cccccccctg
     3001 ctttattcct ggacggaatt gttatgaaaa agcaccttct gcttctcgct ctgctgttat
     3061 ccggaatatc tccggcccag gcgctggatg tcggcgatat atcatcgttt atgaacagtg
     3121 acagcagcac gctgagcaaa acgatcaaaa acagtaccga cagtggtcgc ctcatcaata
     3181 tccgtctcga acggctctct tcaccgcttg acgacgggca ggttatcgca atggacaagc
     3241 cggatgagtt gctactcact cccgccagct tgctgctacc cgcccaagcc agcgaagtga
     3301 tccgcttctt ctataaggga ccagcagatg aaaaagagcg ctactaccgc attgtctggt
     3361 ttgatcaggc cctcagtgat gcgcagcgcg ataatgccaa ccgcagcgct gtggccactg
     3421 cttccgcccg catcggcacc attctggtcg tcgcaccccg ccaggcaaac taccactttc
     3481 agtacgccaa cggctccctg acaaatacag gaaatgcgac gctgcggatc ctcgcttacg
     3541 gcccctgcct gaaagccgcc aatggtaagg agtgtaaaga gaattactac ctgatgccgg
     3601 gcaagtcgcg tcgttttacc cgcgtggaca cggcggataa caaaggacgg gttgcacttt
     3661 ggcagggtga taagttcatt cccgtgaaat agatagctgt gcagatggat aacgacaatg
     3721 cctttacgac ggttctcccc aggactgaaa gcccagtttg ccttcggcat ggtctttttg
     3781 ttcgttcagc ccgatgccag cgctgctgac ataagtgcgc agcaaatagg tggggtgatt
     3841 attccgcagg ccttcagtca ggcgctccag gacggcatga gcgtcccgct ttatattcat
     3901 ctcgccggta gccagggtcg ccaggacgat cagcgaatcg gcagcgcttt tatctggttg
     3961 gatcatggac agctacgcat ccggaaaata cagctggaag agagtgaaga taacgccagt
     4021 gtcagcgaac aaactcgaca gcagctgatg gctctggcga acgccccgtt caatgaggcc
     4081 cttaccatcc ccctgactga caacgcgcag ctggatctca gcttgcgcca actgctgctg
     4141 cagctggtgg tcaagcgcga agcgctgggc actgtactac gctcacgtag cgaagacatc
     4201 gggcagtcca gtgttaacac cctcagcagt aatctgagct ataacttcgg catctataac
     4261 aaccagttgc gtaacggcgg gagcaacaca tccagctatc tgtcgctgaa taacgttact
     4321 gcactgcgcg aacatcatgt ggtgctcgac ggctcgctgt acgggatcgg tagcggtcaa
     4381 caggacagtg aattatataa agcgatgtat gaacgcgatt ttgccggtca ccgatttgcc
     4441 ggtggaatgc tcgacacctg gaacttgcag tccttagggc cgatgaccgc catttcagca
     4501 gggaagattt acggcctttc ctggggaaac caggccagct ccaccatctt cgacagcagc
     4561 cagtcagcca cgccagtgat cgccttttta ccggcggcgg gcgaagtaca tctcacccgt
     4621 gatgggcggt tactaagcgt tcagaacttc accatgggca atcatgaagt ggatacccgg
     4681 ggtctaccgt acggtattta cgatgtggaa gttgaggtga tcgttaacgg tcgcgtgatc
     4741 agcaaacgca ctcaacgggt caataagctg tttagccggg ggcgcggcgt cggtgcacca
     4801 ctggcgtggc agatatgggg cggtagcttt catatggatc gctggtcgga aaacgggaaa
     4861 aagacgcgac cagctaaaga gagttggctg gcaggtgcct cgacctccgg ctcactgagt
     4921 acgcttagct gggcggcaac gggatatgga tacgataatc aggcggtggg tgaaacccgt
     4981 ctgacgctgc cgcttggggg agcgatcaac gttaacctgc aaaatatgct ggccagtgac
     5041 agctcatgga gcaacatcgc cagcatcagc gccactctac cgggaggctt tagttcgctg
     5101 tgggttaacc aggaaaaaac ccgcattggc aatcaattgc gacgtagcga tgccgacaac
     5161 cgtgcaatcg gcggcacact caacctgaac tcactgtggt cgaagctggg cacattcagc
     5221 atcagctaca atgatgaccg ccgttacaac agccattatt acacggcaga ttactatcaa
     5281 aatgtctaca gcggtccctt tggttcgctt ggcctgcggg ccggtattca gcgctataac
     5341 aacggcgaca gcaacgccaa tacagggaaa tatatcgctc tcgatctctc gctaccactg
     5401 ggcaactggt ttagcgcagg gatgactcat caaaacggct acaccatggc aaacctgtca
     5461 gcacgcaagc agtttgatga agggaccatt cgcactgttg gtgccaatct gtcacgagcc
     5521 atctccggcg ataccggtga tgacaaaacc ctcagcggtg gggcgtatgc acagttcgac
     5581 gctcgttacg ccagcggaac gctgaacgtc aatagcgcgg cggacggcta cgtcaatacc
     5641 aatttaaccg ccaatggcag cgtcggctgg cagggtaaaa acattgctgc cagcgggcgg
     5701 actgatggca acgctggggt gatattcaac accgggctgg aggacgacgg tcagatcagc
     5761 gccaaaatca acgggcggat tttcccgctt aacggcaagc gtaactatct cccgctctct
     5821 ccctatggaa gatatgaggt ggagttacag aacagcaaaa actcactcga cagttacgat
     5881 atcgtcagcg gccgcaaaag tcatctgact ctctatccag gcaatgtcgc tgtcattgag
     5941 ccagaggtga agcagatggt taccgtctcc ggccgtatcc gtgcggaaga cggcacactg
     6001 ctggctaacg cacggattaa caaccatatc ggccgaaccc gaaccgatga aaacggcgag
     6061 tttgtcatgg atgtggacaa gaaatacccc actatcgatt ttcgctacag tggcaataaa
     6121 acctgcgaag tggcactgga actcaaccag gcgcgcggtg ccgtctgggt cggagatgtg
     6181 gtctgcagcg gcctctcatc gtgggcggcg gtgacgcaga caggagaaga gaatgagagt
     6241 taacctactg atagcgatga taatctttgc gctaatctgg cctgcaaccg cgctcagagc
     6301 tgcagtgagc aaaacaacct gggcggatgc accggcacgc gagtttgtgt ttgtcgaaaa
     6361 caactcagac gacaactttt tcgtcactcc tggcggggcg ctggatccgc gcctgaccgg
     6421 tgccaaccgc tggaccggtt taaaatacaa tggttcagga accatctatc agcaaagcct
     6481 cggctacatt gataacggtt acaacaccgg cctttatacc aactggaagt ttgatatgtg
     6541 gctggaaaat tcaccagttt catctccttt aactggcttg cgctgcatca actggtacgc
     6601 tgggtgtaat atgaccacca gtcttatcct gccgcaaacc accgacgcca gtggatttta
     6661 tggcgcgacg gtgaccagcg gcggcgcgaa gtggatgcac ggcatgttgt cagacgcgtt
     6721 ttaccagtat ctgcaacaaa tgcccgtcgg cagcagcttt acaatgacca tcaatgcctg
     6781 ccagacctct gtgaactatg acgccagcag cggcgcacgc tgtaaggatc aggcctccgg
     6841 caactggtat gttcgcaacg tcacccatac gaaagcagca aatctacggt tgataaatac
     6901 ccactcgctg gcggaagtat ttatcaacag cgacggagta ccgactctgg gcgaagggaa
     6961 cgccgactgc cggacgcaaa ccatcggcag ccgttcagga ttaagttgta agatggttaa
     7021 ctataccctg caaacaaacg gactcagcaa cacctcaatc catatattcc cggcgatcgc
     7081 caactcgtcg ttagcctcgg ccgtcggggc gtacgatatg cagttcagtc tgaatggcag
     7141 ttcatggaaa ccggtgagca atactgccta ttactacacc ttcaacgaga tgaagagcgc
     7201 agactcgatc tatgttttct tctcgagcaa cttctttaag cagatggtga acctcgggat
     7261 cagcgatatc aacaccaaag atctattcaa ctttcgcttt cagaacacca catcaccgga
     7321 gtctggctgg tatgaatttt ctacctccaa cacgctgatt atcaaacccc gtgatttcag
     7381 catcagtatt atctccgatg aatatactca gacaccgtcg cgggagggat atgttggcag
     7441 cggcgagtcg gcactcgatt tcggctatat cgtaaccacc agcggtaaaa cagctgccga
     7501 cgaagtgctg atcaaggtga ccggacccgc gcaggtgatt ggcgggcgct cctattgtgt
     7561 cttcagctcc gatgacggta aggcgaaagt accgttcccg gcgacgcttt cctttattac
     7621 ccgcaacgga gctacaaaaa cctacgatgc cgggtgcgat gatagctggc gtgatatgac
     7681 cgatgcgctg tggctgacca caccgtggac tgatatctct ggcgaagtgg ggcagatgga
     7741 taagaccaca gtcaaatttt cgattccaat ggataacgcc atttcgctgc gtacggtaga
     7801 tgataacggc tggtttggcg aagtcagcgc ttcaggagaa attcatgttc aggcgacgtg
     7861 gcgtaacatt aactaaggcc ctgctgacag cggtctgtat gctggcgaca cctttgacac
     7921 aggcgatttc ggtcggcaat ctgacatttt cgctgccgtc cgagactgac tttgtcagca
     7981 aacgtgtagt gaataacaac aaaagcgcac ggatataccg tattgccatc agtgctattg
     8041 atagcccggg cagcagtgaa ttgcgcaccc gaccggtgga tggtgaactg cttttcgccc
     8101 cccgccagct agcgttgcag gctggtgaga gcgagtattt taaattttac tatcatgggc
     8161 cacgggataa ccgcgagcgc tactaccggg tctcatttcg cgaggtcccc actcgtaacc
     8221 agacaagacg tagcccaacc ggcggcgagg tcagcacgga gccggtggtg gtgatggata
     8281 ccattctggt agtacgacca cgtcaggttc agtttaaatg gtccttcgac aaggtgacag
     8341 gaacggtaag taacactggc aacacatggt ttaagctact gattaagcca gaatgtgatt
     8401 cgaccgaaga ggaaggcgat gcctggtatc tacgtccggg agacgtggtt catcagcctg
     8461 agttacgtca gccggggaat cattatctgg tctataacga caaattcatt aagattagcg
     8521 attcctgtcc ggctaagccc ccttcggcgg actaagactt atccatcggt cgagggaaga
     8581 attccacctc agagccccaa aaatcgttta aatgatgtgg aagcgatcgt caatatcacc
     8641 tgtaacgatg ggctttctca tatgcacacc atagtgttca ggagacacgg aaaggtatca
     8701 ggcagcctgc taacgagtgg tttaatcacg aattagtacg taaaatcggt aacggctgga
     8761 aatcattcaa tactcacact atcgaaagtt ccccagccaa ccgcggtacg ttcttacata
     8821 cgatgtaccg ctgttctctt tacgattttt agctgtactg gtgaattatg agcaatctga
     8881 atccatgcat gacgtgtggt gcctgttgtg catttttccg cgtctctttt tactgggccg
     8941 aagccgacga tgctggcggc actattcctg ccaggctcac tgaacaaata tccccttttc
     9001 accgatgcat gagcggtacc aatcagaaaa acccccgatg tattgccctt gcaggaaccc
     9061 cgggcaaaaa tgcctgctgc acgatatata aaaatcgatc gtccacatgc agagaatttg
     9121 ccatgtctgg tgagaacgga gtcgtcaatg aggcttgcaa tcgtgcaagg gctaaatacg
     9181 ggctgacacc gttataaaca tacaataatt aattgcactg ccccgccagc gataatagcg
     9241 gggcttcgtt tttcaggggt aacaaaaccc gatacttctt ctatttgcca gcaacaatgc
     9301 ctctcttctg tagcgttctt cggatctaca tcatcctgaa gtagcgcgat atactcactg
     9361 actatctgaa tcagaatata aaaagcaatg tttttaacct ataaaaatgg cgctgtattt
     9421 gcgccatttt tatcattcaa tgcattatct gtttgagcct aaagggatct cagggtctgg
     9481 ctcatgagta attctgtttc gtaaatctct gcgaataatt tcaatagacc agaaccagac
     9541 taaatgtcca aaaatttcag aaacattctc ataccacggg agatcaaaca gaggtggcgt
     9601 cagtcccatg agagggaatg aaatcatatg aacaaaaagt tgggctaaag cacctgccag
     9661 taagccctgc cagagtttaa tttttggaaa tacttcagcg accacacaat aaccgacagc
     9721 aaacactatc gaaaagataa tgtgcgtaac accaacccag ttaaagacat gcccggcaaa
     9781 ggtataaaca gccgcattgg gatctgtcag ccccaaccag tctcgaagaa aaatatacgg
     9841 tggattgaga aaattacgcg agcaatcaat ttggcctgca gccctgatta atgattccgg
     9901 gccacacgct gcattaaaca tatccaccgg gctacgtggc ggcaatggaa cttcagcccc
     9961 ccacttcacg aatgcggaaa caacgccagc aatcagccca atgaatgcag caagaccata
    10021 acgtctgcgg ttcggtggag tttgttcaaa tatattcata tctaccctgc ttgtaccatt
    10081 atgttataca cctcttcagg agtattcata aaacaaggca aatgtaaaga actgtattgt
    10141 tttgtataac aagatagttt cctaatcgcc aatgaatata agctccatca tttctcccta
    10201 tttttatatt agaagtgaca gagatttgca gggtgatgca gagctgaaaa tcacaggttt
    10261 ccttattggt ttttgcatcg tacaactaaa gcaataaacc agctcccatc atcatcaaca
    10321 tcccggcgaa catgaattta ttagccgggc tcatagctgc attcgcgttt taagaataat
    10381 cctcctgctg tcgccgacta tgcttaacgt ttaaaaaagc atcagcactc tcgcaacgca
    10441 ctcttatttt cccctttaga ataccggagg cctggtatga gcaaccaagg cgaatacccc
    10501 gaagataatc gggttgggaa gcacgagccg cacgatttaa gtttgacccg tcgcgatctg
    10561 attaaagtga gcgccgcaac agcggcgacc gccgtggttt atcctcattc tacgctggcg
    10621 gcaagcgttc cggcagctac acccgcgcca gagataatgc ccctgacact gaaggtgaac
    10681 ggcaaaaccg agcagcttga ggtggatacc cgaaccacgc tactggacgc tttgcgtgaa
    10741 aatctgcatt tgatcggtac caagaaaggt tgcgatcacg gacagtgcgg agcctgtacc
    10801 gtgctggtca atggtcgcag gcttaatgcc tgcctgacgc ttgcagtcat gcatcagggg
    10861 gccgagatca tcaccattga aggcctgggt tcgccagata atcttcaccc catgcaggcg
    10921 gcctttatca agcatgatgg cttccagtgc ggctactgca cctccgggca aatttgctca
    10981 tcagtagcgg tgctaaaaga gattcaggac ggcattccca gtcacgtcac ggtcgatttg
    11041 gtttccgctc cagaaacaac tgccgatgag atccgtgaac gtatgagcgg caacatctgt
    11101 cgctgtggtg catacgctaa catccttgcc gccattgaag atgctgcggg ggagataaaa
    11161 tcatgaaggc gtttacctat gaacgagtga acaccccagc cgaggcggca cttagcgctc
    11221 agcgcgtacc cggcgcaaaa tttatcgcgg gcgggaccaa tctgctggac ctgatgaagc
    11281 tggaaattga aacgcccacc caccttatcg atgtgaacgg cctcgggctc gataagattg
    11341 aagtgaccga cgcgggtggg ctgcgcatcg gcgcactggt acggaacacc gacctggcgg
    11401 ctcacgagcg cgtgcgtcgt gattacgcgg tactctcccg cgccctgctc gctggcgcat
    11461 ctggtcagtt acgtaatcag gcaaccaccg caggtaatct gctccagcgc acgcgctgcc
    11521 cctattttta cgacaccaat cagccctgca ataagcgcct gcccgggagc ggctgcgcgg
    11581 cgcttgaagg ctttagccgt cagcacgcgg tggtaggcgt aagtgaagcc tgcatagcca
    11641 cccatccgag cgatatggcg gtcgcaatgc ggttgctgga tgcggtggtg gaaaccatca
    11701 cgccggaggg aaagactcgc agtatcacac tggctgattt ttatcaccca ccggggaaaa
    11761 cgccgcacat tgaaaccgcc ctgcttcccg gtgagcttat cgttgcggtg acgttacctc
    11821 caccgctcgg cggaaaacat atctaccgta aggtgcgcga tcgcgcctcc tacgcctttg
    11881 ccctggtatc ggtcgcggcg attattcacc ctgacggcag cgggcgcgtc gcgctgggcg
    11941 gagtagcaca taagccctgg cgcattgagg ctgcggatgc tcagctatcc cagggggcgc
    12001 aggccgtata tgacacgctg ttcgccagcg cccatcccac cgctgaaaac acctttaaac
    12061 tcctgttggc gaagcgaacg cttgcctccg tactggctga agcgagggca caggcatgaa
    12121 atttgataaa cccgcagggg aaaacacgat cgatcagctg aaggttgtcg gtcgtcccca
    12181 tgaccgcatc gacggaccgc tgaaaactac cggcacggca cgctacgcct acgaatggca
    12241 tgaagaagcc cccaacgccg cctatggcta tatcgtcggt tccgccattg ccaaaggacg
    12301 cctcaccgcc cttgatacgg acgccgcgca aaaagcgccg ggcgtactgg ctgtcattac
    12361 cgccagtaac gccggggcac tcggcaaagg cgacaaaaac accgccagac tgttaggcgg
    12421 ccccactatt gagcactatc atcaggccat tgcgctggta gtggccgaga ccttcgaaca
    12481 ggcgcgagcg gcggcctcgc tggtgcaggc acactatcgc cgtaataaag gagcttactc
    12541 cctggcggac gaaaaacagg ccgtcaatca gccgccggaa gacacgcccg acaaaaacgt
    12601 cggtgacttt gacggggctt tcacctccgc tgcggtgaag attgatgcta cctacacgac
    12661 cccggaccag agccatatgg cgatggagcc gcatgcctcg atggccgtct gggatggaaa
    12721 taagcttacc ctctggacct caaatcagat gattgactgg tgccgcaccg atctggcaaa
    12781 aacgctgaaa gttcccgtgg agaatgtgcg tattatctcc ccgtatatcg gcggagggtt
    12841 tggcggcaag ctgttcctga gaagcgatgc gctgctggcg gccctcgccg cccgagcggt
    12901 gaaacgtccg gttaaagtga tgctcccc
//
LOCUS       DAZGSP010000137        12432 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZGSP010000137 DAZGSP010000000
VERSION     DAZGSP010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.138"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            939..2423
                     /locus_tag="ACHK74_004469"
     CDS             939..2423
                     /locus_tag="ACHK74_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFP0223173.1"
                     /translation="MSFGQLCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(2745..3347)
                     /locus_tag="ACHK74_004470"
     CDS             complement(2745..3347)
                     /locus_tag="ACHK74_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223174.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(3434..3640)
                     /locus_tag="ACHK74_004471"
     CDS             complement(3434..3640)
                     /locus_tag="ACHK74_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0223175.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            4391..4540
                     /locus_tag="ACHK74_004472"
     CDS             4391..4540
                     /locus_tag="ACHK74_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015704109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFP0223176.1"
                     /translation="MEKVISHTRYKLTPAEMEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVT"
     gene            complement(4886..5083)
                     /locus_tag="ACHK74_004473"
     CDS             complement(4886..5083)
                     /locus_tag="ACHK74_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223177.1"
                     /translation="MVIRKTVCRDCDNAIPHNTECCPGYVSADPFDYYRNTDRLLCLL
                     TLLPVLILAVVSGVSVFVLLQ"
     gene            5287..5856
                     /locus_tag="ACHK74_004474"
     CDS             5287..5856
                     /locus_tag="ACHK74_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFP0223178.1"
                     /translation="MTYKYNPFWQQRIRETVLHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKRREGKRVHPTTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRDPGSLAGMIKQAWCSALGVDAGCYDTLAHFPVRPAVWLERDDDTGFQ
                     QVLARADYLAKESTKAYGTGERNFGCSRS"
     gene            6115..6498
                     /locus_tag="ACHK74_004475"
     CDS             6115..6498
                     /locus_tag="ACHK74_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP0223179.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHGHECPYVRPERREIRLIKRLQQFVPDALPVVRKASWYCRQC
                     HHDYYGEQYCTHCQTGRFSEEVVAG"
     gene            6495..6920
                     /locus_tag="ACHK74_004476"
     CDS             6495..6920
                     /locus_tag="ACHK74_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP0223180.1"
                     /translation="MNGIKMGLGITPGEHIISANSALSRNIRHCFCLSCRGRLILQTD
                     AQGAWFEHDLHALSAQQKAACVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYRYQPDLYQDCGGAST"
     gene            7169..7366
                     /locus_tag="ACHK74_004477"
     CDS             7169..7366
                     /locus_tag="ACHK74_004477"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP0223181.1"
                     /translation="MHQDDFCIVNPGGCIIHFPANGRYILDIHDPDFSEQYQRLLERL
                     DYLKKPGTKASGQRNSGYSGF"
     gene            7394..7960
                     /locus_tag="ACHK74_004478"
     CDS             7394..7960
                     /locus_tag="ACHK74_004478"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP0223182.1"
                     /translation="MYTESNVKLSCNALWQKCVLATLYRAAATHPEIKLLQFKLIPGQ
                     GINKAIPSLMADFTGELHRLISEYLQCKRRKDQSVEPTPLYYLWSREAVANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQAWGKVLSEDNSITHLVVYPEVLSLVFDAANTYNA
                     EPGVLEQTARAYIESYGAIERHLGCSDF"
     gene            8016..8280
                     /locus_tag="ACHK74_004479"
                     /pseudo
     CDS             8016..8280
                     /locus_tag="ACHK74_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991605.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8346..8543
                     /locus_tag="ACHK74_004480"
     CDS             8346..8543
                     /locus_tag="ACHK74_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0223183.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            9273..10397
                     /locus_tag="ACHK74_004481"
     CDS             9273..10397
                     /locus_tag="ACHK74_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFP0223184.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLGPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
     gene            complement(10539..11813)
                     /locus_tag="ACHK74_004482"
     CDS             complement(10539..11813)
                     /locus_tag="ACHK74_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFP0223185.1"
                     /translation="MIPMKNYLFQTPEDICVQLKKMTHPVTIRTTDIANFWHYLESAT
                     LPVITKSTTTENREVTFLWRSEKALQGVYLLLNRVTDKKDVKKGLMTHIPSTDIWMLT
                     LVLPASYRGSYSFIEIPTDMTQKDIFQLGSRFSPLPGKPDPFNKTAEINIRGFGESVL
                     SLDMAPEQKEWDDTSHKCTGILSTLHSFVAGYQRRIRLYFPLNPTSVPLGLLVLPDAE
                     TWFDRIDITRALDMAITTGRITPMAIMGIDNINESDRMNILGGNKELIFDIAENLIPQ
                     LYRDYPDIVWAGRSNTVLAGQSLGGVTALMAAIYASTTFGTIISHSPSMWWNPDQGSP
                     ILFTENNTSWVSEQILSAPPKDVNIRLGVGSLEGTTVSHVQRLHHSLIAAGLESNLTV
                     YTGGHDYAWWRGAIIDELANYNCRKVSNNNSV"
BASE COUNT         3256 a         2655 c         3053 g         3468 t
ORIGIN      
        1 gatgtcagtg ggagatatta aatttacgtt tataattatc ttacttcttc tgtgtgtttt
       61 ttataagagt gcacacgtaa tgatatctgt tgataccatg tggttaagtg tttcccttcc
      121 tggtcatgcg taatgattaa atgcttttgt gacaccattc ttccggtctg aatatttatc
      181 ccgttctttt ttatttgtgc agtgatactg tcggaagaga attcagtaat gtgaggtgtc
      241 ctggtcagga taatatctgc tgagatatta ttagttgagt gtgtttttac tgagcaaaag
      301 ctggctttta attcctgtac tgaaacgctg tcgctcgtcc ggggttaact gaaactggat
      361 gatgatgtaa aatgaaggct gtttttgtgt ttgtcgaaga agtgttattt taatccattc
      421 tcatcattgg ctggcgatta tacagaagta tttgaagaaa aaatggtgca ttttatctgc
      481 gtcagcaggg aggaagtatt ttattgatgt atcagtatta aatggttttt gttatttata
      541 aatatgtcat cctgtccata gaatattgta ctcagcattc agtgcaggaa ctgtagtctt
      601 ctttatttta ttcttcttca ggaggggggg attcctgaag gcgttattcc cggtgttctg
      661 tttatgattc ttcccggcag gaaaccggta tctgcaatac ccgggatgat gctttttgtt
      721 cgtacgtggt tgtgtaaaat ttgaatagtg gatgtgctct gaagggaagg aggggacaca
      781 cagattcatt ttgtctttct tcagcagcaa aacacactgt ggttattatt tgactataaa
      841 atcaacaaaa atttttacct catattgatt tgactgctat ttttgctatt aatagccaga
      901 attgcagtgc ttttatatct gtgtgtagtg aggttactat gtcattcggt cagctttgtg
      961 atgagtttcc cttgagttcg ctgcctccac tcattcgtga tgccgttatt gaagctcagc
     1021 aaattacgca ggctcccctt gggcttgtgg cagcgtctgc cctgggcgct gtttcacttg
     1081 tctgccagaa tctgattgat gtctgcagac tgaatacact gcgggggccg gtctctcttt
     1141 ttttcctgac gcttgccgag tccggagaac gaaaaaccgc agtggacaaa ttattaatga
     1201 agcccctgta ccagcaggag atgcagttat attccagata caaaagtgaa ctggctgtct
     1261 ggaaaaacaa ggaagagctt ttaaaggctc agaaaaaagc attgttgtca aaactgaata
     1321 aagaactccg caaaggcgcg gatgagtcag aaaccctcag acaactggaa gtacttcaga
     1381 aaaacagtgc ggaagaacct gtgaggtata aattcatttt taatgatgcc acgactgcgg
     1441 caattaagaa tcagctttgt ggtaaatggc gttcagtggg tattatgtca gatgaagccg
     1501 gtattatatt tgatggatat accctgtctg agttgccttt tattaacaaa atgtgggatg
     1561 gctctgtatt gtctgtggac agaaagaatg aacccgaaca gatgattgaa aatgccagaa
     1621 tgacgttatc cctcatggtg caacccggtc tttttgaccg atatatggag cgaaaaggtt
     1681 ctgttgccag ggacagtggt tttcttgccc gttgtcttat cagtaaacct gcaaccacgc
     1741 aggggaaacg ctttattaac ggagctgtga ttccgggagg atcattaacg gcatttcatg
     1801 aacgattaat ggaactggcc agaggcagta ttgaaaaatc cagtgaggat gaacggtatt
     1861 gtctgcattt ttcgcctgaa gcgcaaaaaa tatttattga gcactataat gttctggagc
     1921 aggatttatc cccttccgga cccttaagcc cgtttcgggg acatgtttca aaaaaaacag
     1981 aaaacattgc aagaattgca gcgttatttc agtatttcag ctatggtgaa gggaaaatct
     2041 ctgccgatat catgacgtct gctgtcgtaa taagttcgtg gtacacagat gaatataaaa
     2101 aattatttgc gttacctgat gaatctgaac tgcagcagaa ggacgccgag gagctgttcg
     2161 actggctcat tgaggagtgc cgcggagaat gcccgccccg tgtcaggaaa aattatattc
     2221 tccagtgtgg cccgggccgg ttcagaaaca ggaaaaaact gaacgcattg ctgaacattc
     2281 ttgaatctca gttcaggctt tctgtcgtgc cggaaggaaa aaccatgtat gttctgcttc
     2341 cccagatagc cagtttaaag ttgtctgatg tctctggtat ttttacctcg ggttatcact
     2401 acaataagct gagagcgaag taaccggtga gcagtttttc tccattatgc ttcagactta
     2461 gatgattgat tattatgggg atttcatcac cgatattcag atgtatgaat atcggtgatg
     2521 aactaaagct caggagctgc gtggtggtga tgaatcaaca ggcatataac tgatacccgc
     2581 cggtcagaag ttccagcctg cgactttcct gtctgcgtgc ccgcagtccg gtgttcaggt
     2641 gcagcgtgtg acagaacgca cggagagcga aacggcaggc agacaggccg ttcagtcagc
     2701 cctggcaccc tgcccggata ccagcttccc tgaaacaact ggcatcaggt gcgcttatcg
     2761 tcaaccgttg tgcggtatgc caggagcacg tactgatcca tcagatccgt cagtgctgcg
     2821 ctctgcttct gacggttgat gatccattcc tccagctctt tttctgattt ccgtttcaga
     2881 ttctccgtcc agtcgtcaag gcagtttccg gtcattaaat caagccagca aagccagacc
     2941 agtttgaggc gcaactccgt gccaccaaac tgacggaaga ggctgatcag tttatttgcc
     3001 agtgaacgct gcctcagacg ttcacattca gacaggagaa tgccttccag catcggatga
     3061 tgaaccagca gatgttcact ggtgcataaa ttcactatca gatcatcatc caggggcggc
     3121 gtggtccgtt tgtccggacc ataaatcagc cggtgcagac tctggggtac gatggcaatc
     3181 tccggttttt caccattgaa tgacacccac ccgggcgtca gattatggaa gtagtagata
     3241 ttcggatgac acacgcccca ggatacggcg tgccgggtga agccggtcag ccatttctgg
     3301 tagctttcgt ggagcagttc gtaacgggtc tgatactgtg gtttcatttt catattcctc
     3361 acgggggata acaggcagca gaacgccgcc ggcaggccgg agactgcgtc tgctgcagat
     3421 gaacggttaa gaattacgga cgggactgtg caatacgcgc ctgcagccag gcttccactt
     3481 cgcttttcag ccatcgggag ctgcggccca ttttgatggg cgcaggaaag tcaccatcct
     3541 tgatgagttt gtaaaaccac ttatcggtca ggccggttag ttgagtgata aatgccatgt
     3601 cgaccatctg gtcatccatc agtgaaactg gggtgatcat gagaatgttc ctccggatgt
     3661 tgttaaacca gccggtgtgg tcatgaaaac aacgcaccac cttcaccggc ataacaaccg
     3721 gggagaaacg taaaaaataa tcgggaatgt gaccgccagg agataaacgg ggaggttacg
     3781 gtggctgagc cgtaccggta tgccgggtgg ttgttacgaa acgatgcttc cgcacgcgcg
     3841 cgcgataaag aagggatgat tttcgtgagg aagaggggaa aactggcgtt ctgataattg
     3901 cagagttatt tacggggcgg ttacggtggg gcagcccgtg ccttgtgggc agggacaggt
     3961 atcaccgatg ggataagact gtctgattgg tattataaat atataagaga gatcaaacac
     4021 atgagatcaa atacatgagt tcatatatgt gaaataaaca cggtgagtgt gatcagtggg
     4081 catgatgaga acgcagtagc aacagactgc gctatccctg atagctggcg tataacctgc
     4141 gtatataaaa cgtaccagca acgcacgtga acagagtgca ttaagcatcc gtcgttgaca
     4201 ccgacctggt gagtgacagt ctgagagacc ggttaagtgg tggtacggga gtaatgagtt
     4261 tggggagggt accagaatgt cagtctggta cggtatgctg acagtatcac gttaattgca
     4321 gcataaagtg atgagaggct aatggaagtt aaaaccagag cagttccgtc gttgttcatc
     4381 ccgggagaca ctggaaaaag tgatttccca cacgcgttat aagcttactc cagcggagat
     4441 ggaagccttc aactctgcgg tcgatcaccg actggcagaa ctgacaatga acaagcttta
     4501 cgatcgtgtt cccgcttcag tctggaaata tgtcacctga acgttcggta atcacagagg
     4561 tattgcgcca ggtgttatct gctctgtgtg attctttttg ggttttctca ggttgttaaa
     4621 agctgaatat gttcctgttg tcgggaacca ggataatacc gggaacgtta agcggtatag
     4681 cgacaacaaa gaattcgaaa tcaaaaggga atggtgccgg aggcgtgggc ggtaatgggc
     4741 cgattttcgg gcattaccga cctctgctga ggttttctcg tatttatcgg ggagctggtg
     4801 cgttactgtc actgtctggc acagcaaacg cagaggtggc ggggcgctct gctccgctga
     4861 ctccggctaa tctctcccct tttacctact gcaacagtac aaatacactg acgcctgaaa
     4921 cgacagccag aatcagcaca ggcagtaacg tcagtagaca aagcagacga tcggtattcc
     4981 ggtaataatc gaagggatct gctgacacgt agcccgggca acattccgta ttatgaggga
     5041 tggcgttatc acaatccctg cacaccgttt tgcgtatcac catcacgtcc tcctcacaat
     5101 cttatccgta accggctttt tgcattaaaa aaacaacgct gaaaattcag ccgtcagtcc
     5161 ggagacgaca gtcgggttat ctcagagtgc ctgagatatt gcctggttgg agatcacttc
     5221 agggactctg cgcatttttt acggcgtggt taatccgtat gaacgaaacc atacggagat
     5281 ttaaccatga cctacaaata caaccccttc tggcagcaac gtattcgtga gacggtgttg
     5341 cacgcactgg atgttcatcc ccgtctgacg gcattgcggg ttgatctgcg tctcccagat
     5401 gtaccggcag caacggacgc ggctgtgata tcccgcttca tcaatgccct gaaagcccga
     5461 atcgacgctt accagaagcg taagcgccgt gaaggtaaac gcgtgcatcc cacaacactg
     5521 cattacgcct gggcccggga gttcggggag ctaaaaggca aaaaacacta tcacctgctg
     5581 ctgctggtca accgggatac ctggtgtcgt gcaggtgatt accgcgatcc gggatcactg
     5641 gccgggatga ttaaacaggc ctggtgcagt gccctgggag tggatgccgg atgttatgac
     5701 acgctggcac attttccggt caggccggcg gtgtggctgg aacgcgatga tgacaccggc
     5761 tttcagcagg tgctggcgcg tgctgactat ctggcgaagg agagtaccaa agcttacggc
     5821 accggtgagc gtaattttgg ctgtagccgg agttgagcac gactggcgcg ccaatacccg
     5881 gcaacccaaa agtatgtgtt gttgtctggc ggcaacgatg tatccactcg cccactgact
     5941 gttttttacg tcagagttcc attgtgtgac aaccacagtg ctgtaacagc aaccagcctg
     6001 ttttacccaa actccagtcc agattttaac tgaaccgcca tttacaccct gagcacactg
     6061 cccgtaccgg gacggctgtt gcctgcgtgt cgtctggcgc tgaggaatat gacgatgtac
     6121 gcaaaatcct ttattgcatt tgatggcaac ggacgtctga cgggcgcccg tactgcacag
     6181 gccgcacctt atgctcacta cacctgccac ctttgcggca gtgcactcag ataccacccg
     6241 caatacgaca ctgaacgtcc ctggtttgaa cacactgacg acgggctgac tgagcacggt
     6301 catgagtgcc cttatgtcag accggaacgc agagaaatac gtttgattaa acgtctgcag
     6361 caattcgtac cggatgcctt acccgtagta cgtaaagcca gctggtactg cagacaatgt
     6421 caccacgatt attatgggga gcagtactgc acacactgcc agaccgggcg cttcagtgag
     6481 gaggtggtag caggatgaac ggcataaaaa tggggctggg gatcacgccg ggtgagcaca
     6541 ttatcagtgc caactctgcg ctcagcagaa atatccggca ttgtttctgc ctttcctgtc
     6601 gtggccggtt gattttgcag acagatgctc agggggcgtg gtttgagcat gatttacatg
     6661 ccctgagcgc gcaacagaag gcggcctgcg tggtgctgaa tccggaaaag tcccaccctt
     6721 acattgagga tatggcgatg tttctatcgc cattaccggt cgtgctggag tggcattgtg
     6781 tgatgtgtga gcagtttttt catgggaaga aattctgtga ggcctgtggt acggggattt
     6841 attgcagagc ggtatgtacg aagtctgttt acaggtacca gccagatttg taccaggact
     6901 gcggaggtgc gagtacttaa tccaaaatat ccgctctgcg cagggcgttc agaggtattg
     6961 ccagcctgca ttttatccgg tgtttcgcac ctgccccgtt cgtgacacaa aggcgcgaca
     7021 ccggatatcc gcagtcctgc ttacctgaat aaaacccggg gtcatgattg tgtgctcgtg
     7081 gaacgaaatc gcaccgtgat cgcggattcg ccgaatctgc actgtggtgt caataaacca
     7141 cacgcgagag tcctctccgt attgatcagt gcatcaggac gatttctgca ttgttaatcc
     7201 agggggctgc attattcatt tccctgctaa tggacgttac atcctggata ttcatgatcc
     7261 tgatttctct gagcagtatc agcgcttatt ggagcgtctg gattatctga agaagcccgg
     7321 tactaaagca tcaggccagc gtaactctgg ctacagcggt ttttgacctg ttgtaactgt
     7381 aaggtgattt attatgtata cagaatcaaa cgttaagctt tcctgtaacg ccctctggca
     7441 aaagtgtgtc cttgcaactc tgtatcgggc tgcagccaca catcctgaaa ttaaattatt
     7501 acaatttaaa ttaataccag ggcagggtat taataaggcc attccctctc tgatggctga
     7561 ttttactggt gaactgcata gactcatttc tgaatatttg cagtgtaagc gacgtaaaga
     7621 tcaatctgtt gagcccaccc ctttatatta tctctggagt agggaagctg tggctaacga
     7681 taccactcct tatcttatgg tgctgctact taataacgat gtctggtgta agagtcgtga
     7741 ggtggtggtt ctaagaaatc tgattgagca ggcgtgggga aaagtattga gtgaagataa
     7801 ctccattaca catctggttg tgtatcctga agtgttgtcg ttggtatttg atgcagctaa
     7861 cacatacaac gctgagcctg gtgtactgga acagacggcc cgggcttaca ttgaatccta
     7921 tggtgccatt gaacgtcatc ttgggtgtag tgacttttaa cgtcaggtga cgcattgtca
     7981 cttcagacct gctacctgta cgccggaata taatggtcag agaggcatat ttcgacgccc
     8041 ctaatctggg gcgtttgatt caggatgtct ggaacaatcc gggctttgtg tcaccgggcg
     8101 catcaaagct gtaccacccg ggatgtggcg ctggatcgtc atttaccgga ctgcgaagac
     8161 atacttaaag aggtgatatg ggcgttcagt gattttgtcc gcgataatcg tggtgtgtat
     8221 gatccggaag cccgttaccc cgccggtaat ccctggtatc ccgtaatagg ccagttctga
     8281 aagcacagct cagcagattc cccgcattcg cacaatcttc ataaatctca caggacattc
     8341 tgaccatgtt gactacaaca agccacgaca gcgtatttct gcgtgccgat aattccctga
     8401 tcgacatgaa ctatatcacc agtttcaccg gtatgaccga caaatggttt tacaagttga
     8461 tcagtgaagg ccatttccct aaacccatca aactggggcg cagcagccgc tggtacaaaa
     8521 gtgaagtgga gcagtggaag tgaagtagag cagtggatgt agcaacgaat cgaggaatcc
     8581 tggggggcag cagcatgaaa cgtgttgtaa tgccggtacg ctggcaatgt gcaaaatgcc
     8641 agcgctggta ttgtggaacc cggccctgcc cttggtgctg gcgatattcc cgttcatctt
     8701 cctgctgata ccttccgttc agcagcgggt tgttatccct tcctgactga ttcgtcattc
     8761 cattcttatt gattagaact ggcattacac cggtgccggc gtgctttcct gcgtgtctgc
     8821 gccggttcag taaattagaa aagaaacatt tgtcagcgcc agtgatataa ggcgtaacgg
     8881 ctaacaatct tggcgttaat acattccgag caaataaccg aagcgttact tttagtgacg
     8941 gggttatcca atacttttct agtcagtgtt tagtatttat gcaagcgata taagtagtca
     9001 ccgcttagac tattgacatt catgatgcct gttaattcaa atttcaggta tgaacagtca
     9061 tttattgttg tgatactgat tccgctagtt tagcataact gtcctgcaaa tcatggttca
     9121 aaagagaaag ctcctacaaa ctgtatcctg tatctctacc agaagataat atacttggtt
     9181 atttttgtgc ataagctcac ataaaaataa tattaattat gttttcttag agatagaaaa
     9241 ataaacaggt aagcagaaga cggacttttt taatgcgaaa acgtattctt tttattggcc
     9301 caccgctgta cggtttgtta tacccattga tttctctggc tcaggccttt cgtgtaattg
     9361 gacatgatgt agtaattagt agcgctggca aattcgcgaa taaagcagca gaagctggac
     9421 tggttgtttt tgatgcagtt ccaggtttag attcagaggc tggatatcgc catcaggaag
     9481 agttgaggaa aaaaagtaat attattggtc atttctcttt ttttagcgat gaaatggcag
     9541 ataacctcat cgattttgca ggaaaatgga ggccagattt aatagtctat ccccctcttg
     9601 gtccggcagg cccattggtt gctgctaaat atagaattcc ttcagtgatg ctggctgttg
     9661 gattcgcgca tacatctgcc catattcaga tgttaaaccg ttctttaagc aatgcttaca
     9721 ggcggcatgg agtcagcggt ccactatgtg atttagcatg gattgatgtt gctcccccaa
     9781 gtatgagcat tcttaaaaat gctggagaac cggttatctc aatgagatat attccttata
     9841 acggaggtgc tgtaaaggaa acatggtggg acagggattc tgatcggaaa cgtttactta
     9901 tcagccttgg cactgtaaaa ccaatggttg atggtctgga gctgatttca tgggttatgg
     9961 attctgcaaa tgaagttgat gctgatatta ttttgcaact tgcaataaat gctcgtactg
    10021 gtttacgaaa actaccatca aatgtacgtc tggttgactg gatacctatg ggggtattcc
    10081 ttaatggagc tgatggattt attcatcacg gtggcgcagg taataccctg acagcgctgt
    10141 atagtggaat accacagatt gtgtttggcg aaggtgcaga tcgctctgtt aatgcagaaa
    10201 ttgttgcgaa gcgtgggtgt gggattattc cggacaagca tggactgact agtgatttgg
    10261 taaatcgcct gctttatgat gattcactac gcttctgttc agatcaggta gccgctgaaa
    10321 tggctgaaca acccagtcct gcagagattg cagaggtttt gatgagaaaa ttaaaaaaca
    10381 acgggaaaca attgtagcgt attgctgaaa ttttcaacga gaaatgttat gagtaatata
    10441 atgtccttca tcacataaat gaaggacatc actaaataaa tgagatagtc tcccataata
    10501 atattgcaga aagagttatc ggacgataaa aagatatttt acacagagtt attatttgat
    10561 accttcctgc aattataatt tgctaattca tcaataattg ctccgcgcca ccaagcataa
    10621 tcgtgaccac cggtatagac agtgaggtta ctttccaaac cagctgcgat taacgaatga
    10681 tgcaatcgct gaacatgtga gacggttgta ccttctaaag aaccgactcc aagtcgaata
    10741 tttacatctt tcggaggcgc tgaaagtatc tgctcactta cccaggaggt attattctca
    10801 gtaaacaaga tcgggctgcc ctggtcaggg ttccaccaca ttgaaggtga gtgactaatg
    10861 attgtaccaa atgttgtcga cgcatatata gctgccatca gtgctgtcac tccaccgagg
    10921 ctctgcccgg ccagtacagt attagagcga ccagcccata cgatatccgg atagtctctg
    10981 tataactggg gtatcagatt ttccgctata tcaaagatga gttctttatt gcctcccagt
    11041 atattcatac gatcagattc attaatattg tctatcccca taattgccat tggcgtgata
    11101 cgaccagtgg tgatggccat atctaatgcc cgggtaatat ctatccggtc aaaccatgtt
    11161 tcagcatcag gtaacacaag taatccaaga ggtactgatg ttggattcag gggaaaatat
    11221 aaacgaatcc ggcgttgata tcctgcaaca aaggaatgta atgttgaaag aatacctgta
    11281 catttatggg aagtatcatc ccattccttt tgttcaggag ccatatcaag agaaaggact
    11341 gattctccga atcctcgtat atttatttct gctgttttgt taaatggatc aggtttaccg
    11401 ggtaatggag agaagcgact tcctagttga aatatgtctt tttgtgtcat atctgtggga
    11461 atttctataa atgagtatga gccccgatat gaagctggta acaccagtgt cagcatccag
    11521 atatctgtcg aagggatatg agtcattagt ccttttttga cgtctttttt atctgttaca
    11581 cgattcagga gaagatatac gccttgcagt gctttctctg agcgccacag aaatgtaacc
    11641 tcccgatttt ctgtagtggt gctttttgtg atcactggaa gtgttgctga ctcaagatag
    11701 tgccagaaat tagcaatatc tgttgttctt attgtgacag gatgtgtcat tttttttaac
    11761 tgtacacaaa tatcttcggg tgtctggaaa agatagtttt tcataggtat catcttaaat
    11821 taatccaagt gtaagaataa tacatcaaat gacgttcatt caagggatga tgagtgactt
    11881 tccggaatta aatctgttgt attgatgata aatataaaac aggtttaata aaatttttgc
    11941 tttatactgt agatttcacg tatgtaagta atatatgaaa aggctctcgt taagatgtct
    12001 ggtacaaaat gacagatcat atgcctttgt aaagagacga cagaagtaga gtcttacagg
    12061 cacatcataa ataccctccg gccatgccgg agggtattta tttaactgcc gctgtttgca
    12121 ctggatgtgg aactactcat actataagaa ggtccacttc cccccggata catgatgatg
    12181 gatggagtac ccggagccca gcatgctcca ttagcatcaa tatatgttcc catatctttt
    12241 cctcgtagat ctattacatt agtagaacta ggtaatccat taagattcat ccctcctttc
    12301 acgaaatcaa gcatgtgatc tgatatttct ctcattttaa agacctcctg ctcatttatg
    12361 ttcagatatt aatttggtgt tgcaaccatt tattatcaca gaattcagaa tatcattcag
    12421 ttttattttt gt
//
LOCUS       DAZGSP010000138        12126 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZGSP010000138 DAZGSP010000000
VERSION     DAZGSP010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.139"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            315..1085
                     /locus_tag="ACHK74_004483"
     CDS             315..1085
                     /locus_tag="ACHK74_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFP0223186.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1076..1549
                     /locus_tag="ACHK74_004484"
     CDS             1076..1549
                     /locus_tag="ACHK74_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFP0223187.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1656..2195
                     /gene="dnaT"
                     /locus_tag="ACHK74_004485"
     CDS             1656..2195
                     /gene="dnaT"
                     /locus_tag="ACHK74_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFP0223188.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2198..2935
                     /gene="dnaC"
                     /locus_tag="ACHK74_004486"
     CDS             2198..2935
                     /gene="dnaC"
                     /locus_tag="ACHK74_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFP0223189.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2984..3478
                     /gene="yjjA"
                     /locus_tag="ACHK74_004487"
     CDS             2984..3478
                     /gene="yjjA"
                     /locus_tag="ACHK74_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFP0223190.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQMLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3732..6023
                     /gene="opgB"
                     /locus_tag="ACHK74_004488"
     CDS             3732..6023
                     /gene="opgB"
                     /locus_tag="ACHK74_004488"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFP0223191.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRHR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTMWKGKTAFKDTVIDMARYKSNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6162..7184)
                     /gene="lgoD"
                     /locus_tag="ACHK74_004489"
     CDS             complement(6162..7184)
                     /gene="lgoD"
                     /locus_tag="ACHK74_004489"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFP0223192.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7323..8237
                     /locus_tag="ACHK74_004490"
     CDS             7323..8237
                     /locus_tag="ACHK74_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFP0223193.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8452..9813
                     /locus_tag="ACHK74_004491"
     CDS             8452..9813
                     /locus_tag="ACHK74_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0223194.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITDFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9862..11517)
                     /gene="tsr"
                     /locus_tag="ACHK74_004492"
     CDS             complement(9862..11517)
                     /gene="tsr"
                     /locus_tag="ACHK74_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFP0223195.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(11636..12082)
                     /gene="hpaR"
                     /locus_tag="ACHK74_004493"
     CDS             complement(11636..12082)
                     /gene="hpaR"
                     /locus_tag="ACHK74_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFP0223196.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
BASE COUNT         2952 a         3196 c         2949 g         3029 t
ORIGIN      
        1 gaaaaatcac acaaccaata tttattcaat gaaatctgat ggttttaact tgttaacatt
       61 aaaaaacgtg cccggttatc tattatcctg attcgtaaag gccaaacaat aaattcatta
      121 tagtgataat attcatgtga tagtttgttt gcggcgagag ataattcgct ttttatcacc
      181 gagcttgtcc cgataaccag gattaatcag cgcaattttg atcttaacct accgcaaaac
      241 aaccgtgctt catgatacaa atggattctc attatctgaa caatacgcag cacgtctatg
      301 acaaagggcg agttatgcaa actgagcaac agcgagccgt aacgcggctt tgtatccagt
      361 gtggattatt tcttttgcaa catggtgcgg aaagcgcgtt ggttgatgag ctttcctcac
      421 gactgggtcg ggcgctggga atggacagcg ttgaaagttc tatctcttcg aacgccatag
      481 tgctgacaac cattaaagat gggcaatgcc tgacatcgac acgtaaaaat cacgatcgcg
      541 gcattaatat gcatgtggtg actgaagtcc agcacattgt gattctggcg gagcaccatc
      601 tgctggatta caaaggcgta gagaaacgat ttagccaaat tcagccatta cgttacccaa
      661 gatggctggt agccttaatg gttggccttt cttgcgcctg tttctgtaaa ctcaataacg
      721 gtggctggga tggtgccgtc atcaccttct ttgccagtac ggccgcgatg tatatccgcc
      781 agctgctggc acaacgtcat cttcatccac agatcaactt ttgccttacc gctttcgccg
      841 ccaccaccat ttccggattg cttttgcaac tccccacttt cagcaatacc cccaccattg
      901 cgatggccgc cagcgttctg ctgctggtgc cgggctttcc gttgattaat gccgtcgccg
      961 atatgtttaa aggccacatc aataccggac tggcacgctg ggcgatcgcc agtctgctga
     1021 cactggctac ctgcgtcggc gtagtgatgg cactgacgat ttgggggcta cgcggatggg
     1081 tgtgatcgaa tttctgttag cgttggcgca ggatatgatc ctcgccgcca ttcctgcggt
     1141 cggctttgcg atggtgttca acgttcccgt tcgggcgtta cgctggtgtg cgctgcttgg
     1201 cgcgattggt catggttcac gaatgatctt aatgaccagc gggctgaata ttgagtggtc
     1261 aacttttatg gcttctatgc tggtcggcac cattggtatt caatggtcgc gctggtatct
     1321 ggcgcatccg aaagtgttta ccgtggcggc cgttatccct atgttcccgg gcatatcggc
     1381 ttataccgca atgatttcgg cggtaaaaat cagccagtta ggttacagcg aaccgttgat
     1441 gattaccctg ttaaccaact ttcttacagc ttcatcgatt gttggtgcgt tatccatcgg
     1501 tctttccatt cctggattat ggttgtaccg caagcgccct cgcgtataaa attgcctcgc
     1561 ttaagcacac ggatgagaga cagcctcctc tcctccgtgt gttactataa aagttatctc
     1621 ccttctcgtt catcgttcca tatttgagaa acagtatgtc ttccagagtt ttgaccccgg
     1681 acgtcgttgg tattgacgcc ctggtacacg atcaccaaac cgttctggca aaagctgaag
     1741 gcggtgtggt tgccgtattt gctaacaatg ccccggcgtt ttatgccgtc acgcctgcac
     1801 gcctggctga actgctggcg ctggaagaaa agctggcgcg tccgggaagc gatgtcgctc
     1861 tggacgatca actctatcag gaaccgcaag ccgctcccgt tgctgtcccc atggggaaat
     1921 tcgccatgta tccggactgg caacccgatg ccgactttat ccgcctggcg gcgctatggg
     1981 gcgtggcgct aagagagccg gtgaccaccg aagaactggc ctcattcatt gcctactggc
     2041 aggcggaagg taaagtcttt caccatgtgc agtggcaaca aaaactggcg cgcagcctgc
     2101 aaatcggtcg tgccagcaac ggcggactgc cgaaacgaga tgtgaatacg gtcagcgaac
     2161 ctgacagcca aattccacca ggattcagag ggtaacgatg aaaaacgttg gcgacctgat
     2221 gcaacgcctg caaaaaatga tgcctgccca tatcaaacct gcattcaaaa cgggtgaaga
     2281 gctactggcc tggcagaaag aacaaggagc gatccgctcc gccgctctcg aacgtgaaaa
     2341 tcgggcgatg aaaatgcagc gcacctttaa ccgctccggt attcggccac tgcatcagaa
     2401 ctgctccttt gagaactatc gcgttgagtg tgaagggcag atgaatgcgt taagcaaagc
     2461 gcgccagtac gtcgaagagt ttgacggcaa catcgccagc ttcatctttt ctggtaagcc
     2521 aggaaccggc aaaaaccatc tggcggcggc aatctgcaac gagctgctgc tacgcggtaa
     2581 atccgtattg atcatcaccg tggccgatat tatgtcggcg atgaaagata ccttcaggaa
     2641 tagcggtacc agtgaagaac aactgcttaa cgatctgagc aacgttgatc tgctggtgat
     2701 cgatgagatc ggcgtgcaga ccgaatcgaa atacgaaaaa gtgatcatca accagatcgt
     2761 cgatcgccgc tcttcttcca aacgcccaac cgggatgctg accaacagta atatggaaga
     2821 gatgaccaag ttgctgggcg aacgcgtgat ggaccgtatg cgcctgggta acagtttgtg
     2881 ggtgatcttc aactgggata gctaccgtag ccgggtaaca ggtaaagagt attaatcccc
     2941 gtttctggcg gtatactgcg acacaattcc accttgagta atgatgatga aaactgttaa
     3001 acatcttctg tgctgtgcca ttgccgccag cgcattaatt tccaccgggg tgcatgctgc
     3061 gtcctggaaa gatgcgctct ccagcgccgc cagcgaactt ggcaaccaaa acagcacgac
     3121 acaggaaggc ggttggtcgc tcgcgtcatt aactaacttg cttagcagcg gaaaccaggc
     3181 cttaagcgca gataacatga acaatgccgc aggcattctg caatactgcg cgaagcaaaa
     3241 gctggcttcg gtaaccgatg ccgaaaacat caagaaccag atgctggaaa agctgggcct
     3301 gaacagtgaa gagcaaaaag aagacaccaa ctatctggac ggcattcagg gtttgctgaa
     3361 aaccaaagat ggtcagcaac tcaatctgga taacatcgga acgactccgc tggcagaaaa
     3421 ggtgaaaacc aaagcctgcg atctggtgtt aaagcagggg ctgaacttca tttcctgaac
     3481 cgcgaaacgc cgcgttttag ctgttacccg gctgacgcgg ctctctcctg cccttttccc
     3541 tgctcctgga cggttttccc cctgttacac gggaagcgac cgcgatcaaa aaagtctgtt
     3601 tataaactaa ttcctaacaa catcgcattt ttttgacact agaattgcag caatatggcg
     3661 acgccattac attgtgcttc tcaaaaagct taaacgttgg ctaaacggcc cggtttagtg
     3721 aggatcgccc gttgtcagaa ctactctctt tcgccctttt tctcgcctct gtgctgattt
     3781 acgcatggaa agcgggacgt aacacctggt ggtttgcagc cacattaacg gtgctggggc
     3841 tatttgtcgt tttaaatatc accctgtttg ccagcgacta ttttactggc gatggtatta
     3901 acgacgcggt tctctatacc ttaaccaaca gcctgaccgg tgctggcgtc agcaaataca
     3961 tcctgccggg tatcggcatt gtgctggggc tgacagcggt gttcggtgcg ctgggctgga
     4021 tcctgcgccg tcatcgccat catccgcacc attttggtta cagcctgctg gcgctcttac
     4081 tggcgctggg ttcggtggac gccagcccgg catttcgtca gataacggaa ctggtgaaat
     4141 cccagtcacg cgatggcgac ccggactttg cggcttatta taaagagccg tcgaaaacta
     4201 tccctgaccc gaaactcaac ctggtttata tctacggcga aagtctcgag cggacctatt
     4261 ttgataacga agctttcccg gatctcacgc ctgaactggg cgcgttgaaa aatgaaggcc
     4321 tggatttcag ccacacgcag cagctgccag gaacggatta cacgattgcg ggcatggtgg
     4381 cttctcagtg cggcataccg ctgtttgccc cctttgaagg caacgcctcc gcctctgtct
     4441 ccagcttctt cccgcagaac atctgtctgg gcgatatcct gaaaaactcg ggttatcaga
     4501 actatttcgt gcagggcgcg aatctgcgtt ttgccggtaa agatgtgttc ctgaagtcgc
     4561 atggcttcga ccacttatac ggctcagaag agctgaaaag cgtggtggcc gacccgcact
     4621 atcgcaacga ctggggattc tacgacgata ccgttctcga tgaagcgtgg aaaaagtttg
     4681 aagagctttc ccgctcaggt cagcgattct cactgtttac cctgacagtc gatacccatc
     4741 acccggatgg ttttatctcg cgtacctgta accgcaaaaa atatgatttt gacggtaagc
     4801 cgaaccagtc attcagcgcg gtaagttgca gccaggagaa tatcgcggcg tttatcaaca
     4861 aaatcaaagc gtcaccgtgg tttaaagata ccgtcatcgt cgtctcttct gaccatttag
     4921 cgatgaacaa cacggcgtgg aaatacctta ataagcagga tcgcaataac ctgttttttg
     4981 tcattcgtgg cgacaagccg cagcaagaga cgctggcagt gaagcgtaac acgatggata
     5041 acggcgcgac ggtgctggac attctcggtg gcgataacta tctcggactt ggtcgtagca
     5101 gtttatccgg gcagtcgatg tcggaaatct tcctcaatat caaagagaaa acattggcgt
     5161 ggaagccgga tatcatccgc ctgtggaaat tccctaaaga gatgaaagag ttcactatcg
     5221 accagcagaa aaacatgatt gccttctcgg gtagccattt ccgtttgccg ctgctgttgc
     5281 gggtttcaga caagcgcgta gaaccgctgc cggaaagtga atactcagca ccgctgcgtt
     5341 tccagctggc cgatttcgct ccacgcgaca atttcgtctg ggttgaccgt tgctacaaga
     5401 tggcacaact ctgggctccg gaactggcac tctccaccga ctggtgtgtc tcgcaggggc
     5461 agcttggcgg tcagcaaatt gtccagcatg ttgataaaac aatgtggaag ggcaaaacgg
     5521 catttaaaga tacggtcatc gacatggcgc gttacaaaag caatgtcgat acgctgaaaa
     5581 ttgttgataa cgatattcgc tacaaagccg acagtttcat cttcaacgtc gccggtgcgc
     5641 cagaagaggt gaaacagttt agcgggattt cccgtccgga gtcgtggggc cgctggtcca
     5701 acgctcagct gggcgatgaa gtaaaaatcg agtacaagca tccgctgccg aagaaatttg
     5761 acctggtgat taccgccaaa gcatacggca ataacgccag ccgtcctatt ccggtacgcg
     5821 taggcaatga agaacaaact cttgtgctgg gcaatgaagt aaccaccacc acactgcatt
     5881 tcgataaccc aaccgatgcc gacacactgg taattgtgcc gccggaacct gtctcaacca
     5941 acgaagggaa tatcctcgga cactcgccgc gtaagctcgg gatcggcatg gtggaaatta
     6001 aagttgtgga acgtgaaggg taatttctga ctgatgtgta ggtcgcataa ggtgcgacct
     6061 acacaccttc tccggcctac aaaacatcat tgagaaacag aaaaaaacag cgcccaacac
     6121 ttccccggac gctgcatggc aactttaata atctcgcctt atcagaaagt aattacaccc
     6181 ttaatcaact cacgattgtt aatcacatcg cgctcgtagg tttctgccag cgtggcgaac
     6241 ggatagcgat gggttaacat catgtcggca gtgattttcc cttctgccat cagtcgaccg
     6301 actttagcga aatcttccgg cgtggcgttg cggctgccca tcatcgtcgt ttctttttta
     6361 tggaattccg gatcggaaaa ctgcaactca cctttaaaca ggccgacaaa taccaccgtg
     6421 ccgccgtgac gaatcagatt cacggtgtta ttcatcgcat gttgattacc tgtcgcgtcg
     6481 atcactttct gcgccagcga accaccaaac tgcgcccgta gctgcgcgtc aaaatcttcg
     6541 gctgacgggt ccagtaaagg taattccaga cgcgttgcca catgttcacg gcgcgccgga
     6601 ctggtatccg ccaccaccac ctgtgcgcca tcggctttag cgattgccgc cgcgcccaga
     6661 ccgattggcc ccgccccgac caccagcacc tgctcgccgg gagcaatggc tgcgcgacgc
     6721 accgcatgag cgctaatagc gaaaggttca atcaatgctg ccgcctgcgg gtcaataccg
     6781 tctgcgggca aaatgttcgc caccggcacg ctcaaatact cactaaaacc gccatcctga
     6841 tgcacgccaa tgactgaaat tttttcacag caattggtac gcccgctttt acacgccggg
     6901 cattgctgac aggcaacata agggatcaca gcaacttgct gaccattttt aagatcagca
     6961 atatttttac ccagcccaac aatctcccca catatttcat ggcctaaaac acgtggataa
     7021 ctaaaaaatg gttgatttcc accccaggca tgaatatcgg taccgcaaat cccgacagac
     7081 ttaattttta ttaatgcttc attgtcaccc ggaataggta tctcgcgttg tttccagact
     7141 aattctttcg gctgctggca aattaaaaca ttcatcgtag acatgattcg gctctccatt
     7201 ttttgttgcc ttagatatca atgaaaatga aaaatggcag cggaacggaa actctgtttt
     7261 gtgaaaacac acgcataaaa atgttttaaa tcgggtttta atggactcac aaggaggaaa
     7321 atatgagtcg ttcacaaaat ttacgccaca atgtgattaa ccaggtcatt gatgatatgg
     7381 cgcggggtca tatcccttcc ccacttccat cgcaaagtgc gctggcggaa atgtacaaca
     7441 tcagccgcac cacagtgcgt catattctca gccatttacg cgaatgcggc gtcttgacgc
     7501 aggttggaaa cgactatgtt attgcgcgta aacctgacca cgatgacggt ttcgcctgta
     7561 ctaccgcttc aatgagtgaa caaaacaaag tgtttgagca ggcttttttc acgatgataa
     7621 accagcgcca gttacgccca ggggaaactt tttctgaact gcaactggct cgggccgcgg
     7681 gtgtcagccc ggtggttgta agagaatatc ttttaaaatt cggccgttat aatcttattc
     7741 aaagcgaaaa gcgcggccag tggagcatga agcaattcga tcaatcctac gccgagcagc
     7801 tgtttgagct acgcgaaatg ctcgaaacac attcacttca gcactttctc aacctgcctg
     7861 atcatgatcc ccgctggctg caggcgaaaa ccatgctcga acgtcatcgg ctactgcgtg
     7921 acaacatcgg caatagcttt cgcatgttct cgcaacttga cagagatttt cactccttac
     7981 tgctttcagc tgccgacaat atcttttttg atcaatcgct tgagatcatc tcagtcatct
     8041 ttcattttca ctatcaatgg gacgaaagcg atcttaaaca gcgcaatatc attgccgtcg
     8101 atgagcatat gaccattctc agcgctctga tttgccgcag tgaccttgat gccacccttg
     8161 cgctccgtaa ccatttgaat tcagctaaac aatcaatgat tcgatccatt aatgagaaca
     8221 cgcgttatgc tcattaagta cggattaaaa acagcaaact attagcgggt gcacggtttt
     8281 atcaaaagat aaataccggg aaattcccgc ttacctatgc tcacaatcag tcgactaaaa
     8341 atgtttttgt gagcggtagt aaagtcctaa aactttaacc tgccgttaat gtcaaccttg
     8401 cgtaatgcac aattacgtaa acaattaaaa ttataaatac aggagtccgg cgtggaaaaa
     8461 gaaaatatca ccctcgatcc gcgttcttca tttactccat cttcgtcggc agatattccc
     8521 gtgccaccag atggattagt tcaacgcagt acccgaatta aacgcattca aaccaccgcc
     8581 atgttgttat tattttttgc ggcggtaatc aattatctcg accgcagttc gctgtcggta
     8641 gcaaatttaa cgattcgtga agaattggga ttaagtgcca ccgaaatcgg cgctttgctc
     8701 tccgtgtttt cactcgctta cgggattgcg caacttcctt gcggcccact attggatcgt
     8761 aaaggcccac gcctgatgct gggactgggg atgttcttct ggtcactgtt ccaggcaatg
     8821 tctggcatgg tgcacaactt tacgcagttc gtgttggtgc gtatcggtat ggggattggt
     8881 gaagcgccga tgaacccatg cggtgtaaaa gtcattaacg actggttcaa catcaaagag
     8941 cgcggacgcc cgatgggctt cttcaacgca gcttctacca ttggcgttgc cgtaagccca
     9001 ccgattctgg cggcgatgat gctggtgatg ggctggcgcg ggatgtttat taccattggt
     9061 gtactgggga tttttctcgc catcggctgg tatatgctct atcgcaaccg cgagcacgta
     9121 gaactgactg ccgttgaaca agcttatctc aatgcaggta gcgtcaatgc ccgccgagat
     9181 ccgctcagtt ttgccgaatg gcgcagcctg ttccgtaacc gtacaatgtg gggaatgatg
     9241 ctcggattca gtggcatcaa ctacactgcg tggctgtatc tggcctggct tcctggttac
     9301 ctgcaaacag cctataacct ggatttaaaa agcacagggt tgatggcggc tatccctttc
     9361 ctgtttgggg ctgccgggat gctggtcaac ggttacgtta ctgactggct ggtcaaaggg
     9421 ggaatggctc cgattaaaag ccgtaagatc tgcattattg ccgggatgtt ctgttctgcc
     9481 gcctttacgc tggtcgtacc acaagcgaca acatccatga cggcggttct gctgattggc
     9541 atggcactgt tctgtattca ctttgccgga acatcctgct ggggcttgat ccacgtcgca
     9601 gttgcttctc gcatgactgc gtcggtgggc agtatccaga actttgccag cttcatctgc
     9661 gcctcttttg cgccgatcat tactgatttt attgttgata ccacccactc attccgtctg
     9721 gcactaatca tctgcggttg cgtcaccgca gcgggggcac tggcgtacat cttcctggtt
     9781 cgtcagccga tcaacgaccc acgtaaagat taatctcctt atgcccgata acattttgct
     9841 tatcgggcat tttcatggcg attaaaatgt ttcccagttc tcctcgctat ctgccacggc
     9901 cattttacgc ggcgcagctg gcgtcacggt ttttaccaca gccgatgttt cacgctgctg
     9961 ttgctgaatc cggaacactg ccactgcttc ggtcaggcga ctggcctgct cttccagcgc
    10021 ggcggcggcg gcggcagatt cttccaccag cgcggcgttc tgttgagtta cccggtccat
    10081 ctcagcaacc gctaagccaa cctgatcgat accacggctc tgctcatcag aagcagaagc
    10141 tatttcgccc ataatgtccg tcacgcgggt cacagcactg acaatctccg ccattgtttc
    10201 cccggcgctt tcgaccagtg tagagccaac atccactttc cccaccgagt cttcaataag
    10261 gcttttaatt tcacgagccg cctgagcgct acgctgggcc agattacgca cttctcccgc
    10321 aaccaccgca aaaccgcgcc cttgctcacc cgcacgcgct gcttcaaccg ccgcgttcaa
    10381 agccagaata ttggtctgga aggcaatgcc gtcaattacg ctgataatat cggcgatttt
    10441 ctgcgaactg gtggagatat cgcgcatagt ctgcaccacg ttatctacca ctttgccgcc
    10501 ccgttgcgcc gtttcagaag cacttaatgc cagatggctg gcctggcgcg cattctcggc
    10561 gttctgtttc accgttgcgg tcagttgctc catgctggct gccgtctctt ccagcgaagc
    10621 ggcctgttgc tcggtgcgcg aagagagatc gttattgccg gtagcgattt cgctggcacc
    10681 gctatagatg gcattggccc cgttgcgcac atcaccgacg gtacgcatca gctctccctg
    10741 catatggcgc aaactctctg ccagttgccc catctcatta gagccatcca cctcaatcgg
    10801 tttcaccaga tcgccgcctg caatatgacg gatgctgtca atcaggcgat tcattggcgc
    10861 taccagcgag gctttaatac cgaaccagac ggcgaagatg accgccagta cgacgatcat
    10921 cacgcccacc agaatccaca tcgcctggct gtaggaggca ttgttatcgc tgacggcgat
    10981 atcatagagc cgatcgtttt gctccatgta agccacatac tgcttctcga aaccgtcttg
    11041 atatccctgg gtcggctgat caaagaactc gttgattttg cctgcaccta acagttggat
    11101 cagctccgcc agcgcattgt gataaatatc gtaattacgt ttgatctctg ccgctgcggc
    11161 ggtgctctga cgcgggtcac gcggcaacgc ttcgtaatcc gcccagtttt tttccgcctg
    11221 tttcagcgaa atactggcac tctgcatcag ctcagcaacg gttgaaccgc taccaatatt
    11281 attctgatcc atcatgtagc ggatacccgc gcggttgagg gtgttacgcg tctgcaacaa
    11341 cgcgacccag ctgccattca gcgtggattg ctgctggcga atggtttgta aaacagtgaa
    11401 attttctttg tcattcttta aggcattaaa gaacagaccg cctgatgtca gttgtaaaag
    11461 gccaaaaacg gccaaaacca gcagtaagct ggtcacgatc ttgatacgtt ttaacatggt
    11521 ttctctttcc tgtggaccga tgcaagattt tcggcctgga aagcaaaaac tttatgaaaa
    11581 ggtgtaaccc gccatccatg gaaaaaccag gcgctggtta tcagatacta aaaagttatt
    11641 cattatcgcc cgggatggct tcttgtcggg aattgcccag agcaataaat tcctctaaca
    11701 gatgggttaa ctgttgcatt ttctcggcag taaactgagc ttcaatctgc cgataggctt
    11761 cttcaatctg tgtctgagcg cggttataca gcgcctgccc ctctttggtt agcgagatat
    11821 agagcttccg ctgatcattg atcggcttta atcgcaacac taaaccgtcg cgttccatcc
    11881 gcgtaaggat cccggtcagg cttgggcgca aaatgcaggc gcgatacgcc agatcgtgaa
    11941 aatccattga tgggctttcc gccagaatac gcacgatgcg ccactgttgc tcagtcaaat
    12001 tatgccgttt aacgattggg cgaaaataac tcatcgccgc ttctcgcgcc tggagcaacg
    12061 caatggttag agagtcgtgc attatctttc ccctgaaaaa ttcaaaatca ttaatataga
    12121 aacagt
//
LOCUS       DAZGSP010000139        12019 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZGSP010000139 DAZGSP010000000
VERSION     DAZGSP010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.140"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..367
                     /locus_tag="ACHK74_004494"
     CDS             <1..367
                     /locus_tag="ACHK74_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFP0223197.1"
                     /translation="NVSGKNNCPTMQLMLFNLFSPALKLNTGLAILSPGAFEVHSDGI
                     DVDNELFHYPIKKAYTPYNIHTYKTEEVVNQRNIKVKNMTLDEINNTYCNNDYYNQAI
                     REEPIDLLDRSFSSSSWPF"
     gene            691..2079
                     /gene="narU"
                     /locus_tag="ACHK74_004495"
     CDS             691..2079
                     /gene="narU"
                     /locus_tag="ACHK74_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFP0223198.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAILGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2161..5901
                     /gene="narZ"
                     /locus_tag="ACHK74_004496"
     CDS             2161..5901
                     /gene="narZ"
                     /locus_tag="ACHK74_004496"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFP0223199.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSSSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5898..7442
                     /gene="narH"
                     /locus_tag="ACHK74_004497"
     CDS             5898..7442
                     /gene="narH"
                     /locus_tag="ACHK74_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP0223200.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVMEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7442..8137
                     /gene="narW"
                     /locus_tag="ACHK74_004498"
     CDS             7442..8137
                     /gene="narW"
                     /locus_tag="ACHK74_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP0223201.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            8134..8814
                     /gene="narI"
                     /locus_tag="ACHK74_004499"
     CDS             8134..8814
                     /gene="narI"
                     /locus_tag="ACHK74_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP0223202.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFVPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLVQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8893..9786
                     /locus_tag="ACHK74_004500"
     CDS             8893..9786
                     /locus_tag="ACHK74_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFP0223203.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9882..10727)
                     /gene="nhoA"
                     /locus_tag="ACHK74_004501"
     CDS             complement(9882..10727)
                     /gene="nhoA"
                     /locus_tag="ACHK74_004501"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFP0223204.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVVSLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10900..11469
                     /locus_tag="ACHK74_004502"
     CDS             10900..11469
                     /locus_tag="ACHK74_004502"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFP0223205.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11473..11700)
                     /gene="pptA"
                     /locus_tag="ACHK74_004503"
     CDS             complement(11473..11700)
                     /gene="pptA"
                     /locus_tag="ACHK74_004503"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFP0223206.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11799..>12019)
                     /locus_tag="ACHK74_004504"
     CDS             complement(11799..>12019)
                     /locus_tag="ACHK74_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0223207.1"
                     /translation="NKLHWRLDVVMNEDDCKIRKGNAAELFSGIRHIAINILTNDKVF
                     KAGLRRKMRKAAMDRNYLASVLAGRRLS"
BASE COUNT         2866 a         3109 c         3121 g         2923 t
ORIGIN      
        1 aaatgtttct gggaagaaca attgccctac tatgcagctc atgttattta atttgttttc
       61 cccagcactt aagcttaata ctggcctggc aattctttcg cctggtgcat ttgaagttca
      121 ctctgacgga atagatgtgg ataacgaatt gtttcactat cctattaaaa aagcatatac
      181 cccatataat atacacactt acaagacaga ggaagttgta aaccagagga atataaaagt
      241 taaaaacatg accttagatg aaataaacaa tacttactgt aataacgatt attacaatca
      301 ggcaataaga gaggaaccga tagaccttct ggacagatcg ttttcctcca gttcatggcc
      361 tttttagtga ataaatcatc actaattcta taagtgtctg gtcttgttga taatatagtt
      421 aatatgtaac cctttagatt cacgatctga agggttgaaa catgacatac gttcaaagat
      481 taacccccgt ttgaaggtta gcttgttagt tacatttagt aacacatatc tggctactta
      541 atccccattc tgcccccgcg gttacgcttt cttatctatt ctttcaatca gcacttcacc
      601 caattcatcc ttaattctaa taactggcta ttgcgcctta cctcctgttt tgtctttttt
      661 cattaccaat gtgtgcatgt gaggaacaat atggcactgc aaaatgagaa aaatagtcgt
      721 tatcttttac gcgactggaa accagaaaat ccggccttct gggaaaataa aggaaaacat
      781 attgctcgaa gaaacctctg gatatcagtc agttgtctac ttcttgcctt ctgtgtctgg
      841 atgctattta gcgcagttac cgttaatctc aataaaatcg gttttaattt tactaccgat
      901 caactctttt tattaaccgc attaccctcc gtttctggcg cattattgcg tgttccctac
      961 tcctttatgg tgcctatatt cggtggacgc cgatggacgg tttttagtac tgcaatcctg
     1021 attattcctt gcgtctggct cggaattgcc gtgcaaaatc cgaatactcc ttttgggata
     1081 tttatcgtta tcgctttgct atgcggtttt gcaggtgcaa actttgcttc gagcatgggc
     1141 aatatcagtt tcttctttcc aaaagccaaa caagggagcg ctcttgggat taatggcgga
     1201 ttaggaaact taggtgtaag tgtaatgcag ctggttgcac cgctggtcat ttttgtacct
     1261 gtatttgcca ttctcggcgt caatggcgta ccgcaggccg acggttcggt gatgtcgctg
     1321 gcgaatgccg catggatttg ggtaccgcta ctggcgattg ccacgatcgc cgcatggtca
     1381 gggatgaatg atatcgccag ttcacgcgcc tcaattgccg accagctccc tgtcttacaa
     1441 cgcctgcatc tctggctgct gagcctgctt taccttgcca ccttcggttc gtttatcggt
     1501 ttttctgcgg gttttgccat gctggcaaaa acccagttcc cggatgtgaa tattctgcgc
     1561 ctggcgttct ttggcccatt tatcggtgcc atcgcgcggt cggttggtgg tgctatttcc
     1621 gataagttcg gcggcgtgcg ggtgacgttg atcaacttta tttttatggc gattttcagt
     1681 gccctgctgt tccttacctt accgggcaca ggctccggta atttcatcgc cttttacgcc
     1741 gtatttatgg ggctgtttct gactgcgggt ctgggaagtg gttctacttt ccagatgatc
     1801 gccgtcatct ttcgccagat aaccatttat cgggtaaaga tgaaaggcgg tagtgatgag
     1861 caagctcaga aagaagccgt caccgaaacg gcggcggctc tgggctttat ctcagccatt
     1921 ggcgcagtgg gcggcttttt tattccgcag gcgtttggca tgtcgctcaa tatgaccggc
     1981 tctccggtcg gcgcgatgaa agtgttttta atcttctaca tcgtttgtgt gctgctgacc
     2041 tggctggttt atggtcggcg gaagttcagc caaaaataag agatcgatgc ctgatgtaca
     2101 accgtgtcgc atccgacaag gcatcagatc ccaattcatt cctggagcag gagtcatgtc
     2161 atgagtaaac ttttggatcg ctttcgctac ttcaaacaaa agggcgaaac ctttgccgat
     2221 ggtcacggac aggtgatgca tagcaaccgc gactgggagg acagctatcg ccagcgttgg
     2281 cagttcgaca aaatcgtgcg ttccacccac ggtgttaact gtacaggctc ctgtagctgg
     2341 aaaatctacg ttaaaaatgg tctggtgacc tgggaaatcc aacagaccga ctacccgcgc
     2401 actcgccctg acctgcccaa tcatgaacct cgcggctgcc cgcgtggcgc aagttactcc
     2461 tggtatcttt acagcgctaa ccgcctgaaa tacccgctca ttcgtaaacg actgattgaa
     2521 ctgtggcgcg aagccctcaa gcaacacagc gatccggtac tggcgtgggc atcgattatg
     2581 aacgatccgc aaaagtgcct gagctacaaa caagtgcgtg ggcgcggcgg gtttatccgc
     2641 tccaactggc aggaactaaa ccagctgatt gccgccgcta acgtctggac catcaaaacc
     2701 tacggcccgg atcgcgttgc cggtttctcg ccgatcccgg cgatgtcgat ggtttcttac
     2761 gccgccggaa cgcgttatct gtcgctgctt ggcggcacct gtttaagttt ctacgactgg
     2821 tattgcgacc tgccgcccgc ctcgccgatg acctggggcg agcaaaccga cgtaccggaa
     2881 tctgccgact ggtataactc cagctacatc atcgcctggg ggtctaacgt accgcagaca
     2941 cgtacgccgg acgcccactt ctttaccgaa gtacgctaca aaggcactaa aaccatcgcc
     3001 attacccctg actactctga agtggccaaa ttgtgcgacc agtggctggc accgaaacaa
     3061 ggcactgata gcgccctggc gatggcaatg ggccatgtga ttttaaaaga gtttcatctc
     3121 gataatccca gcgactactt tatcaactac tgccgccgct acagcgacat gccgatgctg
     3181 gtaatgctgg agcctcgcga cgatggtagc tacgttcccg ggcgcatgat ccgcgcatct
     3241 gacctggtgg atggactggg cgaaagcaac aatccgcagt ggaaaaccgt agcagttaat
     3301 accgcaggtg aattggtagt gccgaacggt tcgattggtt tccgctgggg agaaaaaggc
     3361 aaatggaatc tggaatccat tgccgccggt acggaaaccg aattgtcgtt aaccctgctc
     3421 ggtcaacatg acgctgttgc aggcgtggcc ttcccctact ttggcggcat tgaaaatccg
     3481 cattttcgca gcgtaaaaca caatccggtg ctggtgcgcc aattgcccgt taaaaacctg
     3541 acgttagtcg atggcaacac ctgtccggtg gtcagcgttt atgatttggt actggcgaat
     3601 tacggcctcg atcgcgggct ggaagatgaa aacagtgcga aagattacgc tgaaatcaaa
     3661 ccgtacaccc cagcctgggg tgagcaaatt accggcgtgc cgcgccagta tattgaaacc
     3721 atcgcccgtg aatttgccga taccgcccat aaaacgcatg ggcgctcgat gattatcctc
     3781 ggcgcaggtg ttaaccactg gtatcacatg gacatgaact accgcgggat gatcaatatg
     3841 ctgatcttct gcggttgtgt cgggcaaagc ggtggcggct gggcacacta tgtcggccag
     3901 gaaaaactgc gcccacaaac cggctggttg ccactggcct ttgcgctcga ctggaaccga
     3961 ccaccgcgcc aaatgaacag cacctcgttt ttctacaatc attccagcca atggcgctat
     4021 gaaaaagtct ctgctcagga gttactttca ccgctcgccg atgccagtaa gtacagcggt
     4081 catctgattg atttcaacgt tcgcgccgaa cgtatgggct ggctaccttc tgcgccgcag
     4141 ctggggcgta acccgctcgg gattaaagct gaagccgaca aggccggatt atcccccaca
     4201 gaatttaccg cccaggcgct gaaatcgggc gatttacgta tggcctgcga acaaccagat
     4261 agcagcagca atcatccgcg taatttgttt gtctggcgct ctaacctgct tggctcctcc
     4321 ggcaaaggcc acgagtatat gcagaagtat ctgctgggga ccgaaagcgg gattcagggc
     4381 gaggaactcg gtgccagcga cgggatcaaa ccggaagaag tcgagtggca aactgcagcg
     4441 attgaaggca agctcgacct gctggtgacg ctcgacttcc gcatgtccag tacctgcctg
     4501 ttctccgata tcgttctgcc caccgccacc tggtacgaaa aagacgatat gaacacctcg
     4561 gatatgcatc cgtttattca tccgctttct gcggcggtcg atccggcgtg ggaatcacgc
     4621 agcgactggg aaatctacaa aggtatcgcc aaagcatttt cgcaagtgtg cgtgggtcat
     4681 cttggcaaag aaaccgacgt ggtattacaa cccctactgc atgactctcc ggcagagctc
     4741 tcacagccgt gtgaagtgct cgactggcgc aaaggcgaat gcgatctgat tccaggcaaa
     4801 accgcgccga atattgtggc ggtggagcgc gactaccctg ctacgtatga acgctttacc
     4861 tcgctcgggc cattgatgga caaacttggc aatggcggta aagggatttc gtggaatacc
     4921 caggatgaaa tcgatttcct cggcaaactc aattacacca agcgtgatgg cccggcacag
     4981 gggcgtccgc tgattgacac cgccattgac gcttcagaag tgattctggc actggcaccg
     5041 gaaaccaacg gtcatgttgc agtcaaagcg tggcaggcgc tgggcgagat caccgggcgc
     5101 gaacataccc atctggcgct gcacaaagag gacgagaaga ttcgctttcg cgatattcag
     5161 gcgcagccgc gtaaaattat ctccagcccc acctggtccg gtctggaaag cgatcacgta
     5221 tcctataacg cgggatacac caacgttcat gagttaattc cgtggcgcac gctgtcggga
     5281 cgccagcagc tctatcagga tcatccgtgg atgcgtgctt ttggtgaaag cctggtggct
     5341 tatcgcccgc ctatcgacac tcgtagcgtc agtgagatgc gccagatacc gccaaacggc
     5401 ttcccggaaa aagcacttaa cttcctgacg ccgcaccaga aatggggcat tcactcaacc
     5461 tacagtgaaa acctgctaat gctgacgctc tctcgcggtg gaccgattgt ctggatcagc
     5521 gaaacagatg cccgagaact gaccattgtc gataacgact gggtggaagt attcaacgcc
     5581 aacggcgcgc tgactgcccg cgcggtggtc agccaacgtg taccgccggg catgaccatg
     5641 atgtatcacg cccaggaacg cattatgaat attcctggtt cggaagtaac tggcatgcgc
     5701 ggcggcattc ataactcggt cacccgcgtt tgcccgaaac caacgcatat gattggcggt
     5761 tacgcgcagc tggcctgggg ctttaactac tacggcaccg tcggatcgaa ccgcgatgag
     5821 ttcatcatga tccgcaagat gaagaacgtt aactggctgg atgatgaagg tcgcgatcag
     5881 gtacaggagg cgaaaaaatg aaaatccgtt cacaagtcgg catggtgctt aacctcgaca
     5941 aatgtatcgg ctgccatacc tgttcggtga cctgtaaaaa cgtctggacc gggcgcgaag
     6001 gcatggagta cgcatggttt aacaacgtcg aaaccaaacc gggcattggt tatccgaaaa
     6061 actgggaaga tcaggaagag tggcaaggcg gctgggtccg cgatgtgaat ggcaagatcc
     6121 gtccgcgtct gggcagcaag atgggcgtaa taaccaaaat cttcgccaac ccggtggtgc
     6181 cgcagattga tgattactac gaacctttca ccttcgacta cgaacatttg catagcgcac
     6241 cggaaggcaa acatatccct actgctcgcc cgcgttcact gattgacggc aaacggatgg
     6301 acaaagtgat ctggggccca aactgggaag aactgctggg cggcgagttt gaaaaacgtg
     6361 cccgcgaccg caacttcgag gccatgcaaa aggagatgta cgggcagttt gaaaacacct
     6421 tcatgatgta cctgccgcgc ctgtgcgaac actgcctcaa tcccagttgc gtggcgacct
     6481 gcccaagcgg cgctatctac aaacgcgaag aagacggcat tgtgctgatt gatcaggata
     6541 aatgccgtgg ctggcgtttg tgcattagcg gttgtccgta caaaaaaatc tacttcaact
     6601 ggaaaagcgg caagtcagaa aaatgtatct tctgttatcc gcgaattgag tcaggccaac
     6661 ccaccgtgtg ctcagaaacc tgcgtgggtc gcatccggta tctgggcgtg ctgctttacg
     6721 acgccgaccg catcgaggaa gcggcgagca ccgagcgcga agttgacctc tatgaacgtc
     6781 agtgcgaagt gttcctcgat ccacacgatc cctcagtgat cgaggaagcc ctgaaacaag
     6841 gtattccaca aaacgtgatg gaagctgccc agcgttcgcc tgtctacaaa atggcgatgg
     6901 actggaaact ggcgctaccg ctgcaccctg aatatcgcac cctgccgatg gtctggtacg
     6961 ttcctccgct gtcgccgatt cagtcctacg cagatgcggg cggtttgccg aaaagcgaag
     7021 gcgtgctgcc cgccatcgaa agcctgcgta ttccggtgca atatctcgcc aatatgttga
     7081 gtgccggcga taccggtccg gtactgcggg cgctgaaacg gatgatggcg atgcgccact
     7141 atatgcgttc acaaaccgtg gaaggcgtta ctgatactcg tgccatcgac gaagtaggcc
     7201 tgagcgtcgc ccaggtcgaa gagatgtatc gttacctcgc cattgccaac tatgaagatc
     7261 gttttgtcat cccgacgagc catcgggaaa tggcgggcga tgccttcgca gaacgcaacg
     7321 gctgcggttt tacctttggc gacggttgtc acggctcgga cagcaaattc aacctgttca
     7381 acagtagccg tatcgatgcc atcaacatca ccgaagtgcg cgacaaagcg gagggcgaat
     7441 aatgcagatc ctcaaagtga tcggcctgtt gatggagtat ccggacgagc tgttgtggga
     7501 atgcaaggag gacgcgctgg cgttgatccg ccgcgacgcg ccgatgctta cggatttcac
     7561 ccacaacctg cttaacgcgc cgctgctgga taaacaggcc gaatggtgcg aagtgtttga
     7621 ccgcgggcgc accacgtcgc tgctgctgtt cgaacatgtt catgccgagt cccgcgatcg
     7681 cggccaggca atggtggacc tgctggcgga gtatgaaaag gtcggcctgc agctggattg
     7741 tcgggaactg cccgattatc taccgctgta tctggagtat ttaagcgtac tgcctgacga
     7801 tcaggcgaaa gaaggattgc tcaatgttgc gccgatcctc gccctgcttg gcggtcgctt
     7861 aaaacaacgc gaggcaccgt ggtacgcgtt gtttgatgct ctgctgcaac tggcgggaag
     7921 tatactttca agtgacagtg tcacaaaaca agtaaacagc gaagagcgcg atgacacccg
     7981 ccaggcgctt gatgcagtgt gggaagagga acaggtgaag tttattgaag ataacgccac
     8041 ggcgtgtgac agctcgccgc ttaatcaata tcagcgacgc tttagccagg atgtcgcgcc
     8101 gcaatatgtc gacatcagtg cgggaggtgg gaaatgattc agtatctgaa cgtctttttt
     8161 tacgatatct acccgtacat ttgtgcgacg gtgtttttcc tcggcagctg gctgcgctac
     8221 gactacggcc agtacacctg gcgcgcctcc tcaagtcaaa tgctcgataa acgcgggatg
     8281 gtgatatggt cgaatctgtt ccatatcggc attttgggga ttttcttcgg gcacctgttc
     8341 ggcatgttaa cgccgcactg gatgtacgcg tggtttgtgc cagtggcagc gaaacaactc
     8401 atggcgatgg tgctcggcgg tatttgcggt gtgttgacgt tgattggcgg cgcaggactg
     8461 ctgtggcgca ggctgacaaa tcagcgcgtg cgtgccactt ccactacacc ggatatcatc
     8521 atcatgagca ttttgctggt ccagtgtctg ttggggctaa gtaccatacc gttttcagca
     8581 cagtatcctg atggtagcga aatgatgaag ctggtgggtt gggcgcaaag cattgtcact
     8641 ttccgtggtg gatcgtcaga gatgcttaac ggtgtagcgt tcgttttccg cctgcatctg
     8701 gtgttgggaa tgaccatctt cctgctcttc ccgttcaccc gactggtgca tgtgtggagc
     8761 gcgccgtttg agtactttac tcgtcgatat caaattgtgc gttcgcggcg ataatttcac
     8821 cctggccgga tagcccttcg ctctccggcc acttgcaggc tatgatagtt ctccagtcac
     8881 aggagaacgt taatgaaacc gcaggtttac cacgtcgatg cttttacctc tcaaccattt
     8941 cgcggcaatt ctgccggtgt tgttttcccc gccgataatc ttagcgaagc gcaaatgcag
     9001 cttatcgccc gcgaattagg tcattcggaa actgcttttc tgctgcacag cgacgacagc
     9061 gatgtgcgca tccgctactt tacgccaaca gttgaagtgc cgatttgcgg tcacgcgaca
     9121 gtagctgcgc actatgtacg tgcgaaggtg ttaggtttag gaaattgcac ggtctggcaa
     9181 acatcgctgg caggaaaaca tcgcgtgact atcgaaaagc acaacgatga ttatcgtatt
     9241 tcgctggaac aaggtacgcc gggctttgaa ccgccgctgg agggtgaaac acgtgaggcg
     9301 attatcaacg cgcttcatct cactgaggac gatattctgc caggcttgcc aattcaggtg
     9361 gcaacaacag gacactcaaa agtgatgatc ccgttgaaac cagaagtgga tatcgacgcc
     9421 ctttcgcctg atcttgctgc gctgagcgct atcagcaaac agattggctg caatggtttc
     9481 ttcccgttcc agatccgccc aggcaaaaac gaaaccgatg gtcgcatgtt ctcgcctgcg
     9541 attggtattg tggaagatcc ggtgactggc aatgccaatg gcccgatggg tgcatggttg
     9601 gtacatcaca acgtattgcc ccacgatggc aaggtgctgc gtgttaaagg ccatcagggg
     9661 cgcgcattgg ggcgtgacgg cgtgattgaa gtgacggtga caattcgtga taaccaaccg
     9721 gaaaaagtca ccatttcagg cacagcggtg attttgtttc atgccgaatg ggcaattgaa
     9781 atctgaaatc gttgcctgat gcgcttcgcc tgtcaggtct acaaccgggc taatgtatgc
     9841 cggataaggc gtttacgccg catccggcaa cctgacataa attattttcc cgcctccgga
     9901 tgcgtatcaa acgccgccat caccagcgct aactcatcca cagtaaagcc atgtttcgca
     9961 tcatccacgc ccagaccaaa ctgttcctgc atcacggcat ataacgacac cacatccggt
    10021 agatttcgct gctccaccgc gtgcccattt tcataatggg taaaatgaaa attggtcagc
    10081 gttagcttgc caccgtccgg caaatggcgg cacatcagca aatgatggcg aaaatgcgac
    10141 tgcggccagt gcgccgacca gaaattgccc atcacataat cgctttgttg ctgctcgcag
    10201 agatcaaaac ggtacatcga ctgccaatgc tgatgatgat taaactgcaa cacccaatca
    10261 tcaccctcct gcaacaaccg atactctccg tgtggcgtgg tctgcacgag atcggaaact
    10321 aaacgaatcg gcgcggttag cgtctgccca ccgaaaccga catcagcaat ccatttttcc
    10381 tcttccagtt ccaccaacag caaacgatgg gtgcgcggcg gtaatgctgg cggatttgat
    10441 aacactacgc gccctaacaa gctgcgaacg ttaaacccca gctcgcgtaa cacccgctca
    10501 aacacgccat tctgctcaaa acagtaaccg ccacgacggg ctatcaccag tttctcttcc
    10561 agcgattgat catcaagctg tatttccctc ggcagcaaaa cgtcaaggtt ttcaaacgga
    10621 atggtgcaat tgtgtttcag gtgcaatgca cgaagcgtat caatattgac cgcagcagct
    10681 cccgaccagt taatacgggc aaaatagtga ttcagaatgg gcgtcatcag gcgtttccct
    10741 tagtggtttt ctcaacttat aacctggaat agacgccgca ccggctattt tgtgcgatcg
    10801 cggacatgca tcacgtttgc aaatcgccaa tcccgttaat ttcgttactt attgcgttac
    10861 ggactacgct taaaaaaacc ttcttcgcga ggcgatacca tgagccgatt catccccata
    10921 gaattacacc atgccagccg tctattaaat catggcccaa ctgtcatgat caccagtttc
    10981 gatgagcaat cccagcggcg taacattatg gctgcagcct ggtcaatgcc ggtggagttt
    11041 gaaccgccac gcgtggcgat tgtggtagat aaatcgacat ggaccaggga gttgattgaa
    11101 cgtaacggta agtttggcat cgttatcccg ggcgttgcag caactaactg gacgtgggca
    11161 gtgggaagcg tgtcgggtcg tgaagaagat aaatttaatt gctatggcat tccggtggtg
    11221 aaaggtccgg tgcttggttt gccacttgtt gaagagaaat gcctggcgtg gatggagtgc
    11281 cgcctgctac ctgcgacttc tgcgcaacaa aaatacgaca cgctgtttgg cgaggtggta
    11341 tcagcagcgg cagacgcacg ggtatttgtc gaaggccgct ggcagtttga tgatgataag
    11401 ctcaatacgt tgcatcattt aggtgctggc acgtttgtta ccagcggcaa gcgagttacg
    11461 gcgggttaag cattacatgc tgtaaccggg tttctttatc aaagcctcca tttggggcgc
    11521 gatttcggca tcccagatag cttgccaaga ttctggttga atctgctgta gagcaatgct
    11581 tatcgagcta tctttactgt tcagatggcg aataataacg tcggtaatat ctgcagcaag
    11641 tgctgctttt tgttgttcgt ccagttcacg cggaaaacat ttaatgtcga tgtgcggcat
    11701 gattgtgctc cttttgaaag atgtcagtaa aattaacaca cattgtgaat gtcgtgtgtc
    11761 cggcaatgcg tttttacgac aataatgcca gggcaagatt acgaaagcct tctccccgca
    11821 aggactgacg ccaggtagtt tctgtccatg gctgcttttc gcatcttacg tcttaaccct
    11881 gccttgaata ccttatcatt cgtcaaaata ttaatagcaa tgtgccgtat ccctgaaaat
    11941 aattctgctg catttccttt tcttattttg cagtcgtctt cattcattac cacgtccaga
    12001 cgccagtgca gcttattct
//
LOCUS       DAZGSP010000140        11333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZGSP010000140 DAZGSP010000000
VERSION     DAZGSP010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.141"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..425)
                     /locus_tag="ACHK74_004505"
     CDS             complement(<1..425)
                     /locus_tag="ACHK74_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HFP0223208.1"
                     /translation="MQNDDSVTKITWEQLLEEYFFSKNLRPDTEWSYCKVVRVFREFI
                     GDILPGEVTVRDVQRYRRYVLKEQKRREVTWNNKVAHLRAIMGFGIKMKLLPQTENPL
                     VEASVASTEKKKKTLSDNQMLRVYLVVQRFAEAEEQNMV"
     gene            complement(493..1974)
                     /locus_tag="ACHK74_004506"
     CDS             complement(493..1974)
                     /locus_tag="ACHK74_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HFP0223209.1"
                     /translation="MLERLKRLFPVKTECSDVRTDIPGETVPEGYFTPLPVDVLVSTP
                     FRKRCLKQLWENSSMPVDMYQRLCLDPVHQLLSVSQNLPATAEGRWGYTGGFGDLTLQ
                     FTTYAVRLARGRMFPPGATAEEQAAQGSVWNMVVFWSALFYHLPLLAELEGEQADGRC
                     WQPGISVPEGVYRFRFRKMPCQGTEGRQLAALMAGQLLPGEAGAWLATVPGALQNLAG
                     AVWHQHPEMALIRTVLQEAMEEVVSPFAAGDVAEVPVAEVVPVLLQSAAISAESVIQP
                     LPVVQSPVEDAENEGRTAEQEVDEGNETDADTGMLLTLFSAADLSDEGGQIASGVPPD
                     KESGIELPEESGCVTENQGNRIPETVEKTAEFSLTEDSRVESEFIRWLKEGLRDGSLS
                     VNSVDSNVHVVSGYVFLCAPGIFYRFLKESGSGQDRRYVQSLFEREGIHKVRSGERFF
                     QARLYSDAEHSGRYKMVSGYLVKGRFLYGNITVPGDSPLIVFG"
     gene            complement(1967..3457)
                     /locus_tag="ACHK74_004507"
     CDS             complement(1967..3457)
                     /locus_tag="ACHK74_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP0223210.1"
                     /translation="MSFNPTDEQYAIINWQGQQLVVNAFSGTGKTSTLVQFARASPDS
                     RMLYLAYNRAIRDEAERKFPFNVECRTSHQLAYARVGRHYRHRLVPGLRITDVARKLN
                     TRYWALARVAGTGLNHFICSADAVPGLHHLPDKDEMRGVPPADALRAVQLLWNEMSNP
                     GGTFPVTHDTYLKLFQLSGADLSHRWDTILFDEAQDANPVTTALVLGQKCRVVLVGDC
                     YQQIYRFRGANNALSHPALKNADRLWLTQSFRFGPAVARMANLLLQREGETREVKGCG
                     GDDEVLLKCHAREHLQGHYTVLSRTVAGVIATALMAAMKGQKVYWVGGIEGYRTGELE
                     DLYWFQVDMPERMHSDRLRRDYRNFEEYKYIAKSTKDVEMNQSLRLLELCFPLLKKLE
                     LLRQYTVTNEQDADITVSTAHRSKGLEWPVVMLDEDFQDITDPLMTEAERHDETCLLY
                     VAVTRALRTLVLNELMHQLAEEQQKTEGLRGTGYDTSGRVQENGHA"
     gene            3689..5209
                     /locus_tag="ACHK74_004508"
     CDS             3689..5209
                     /locus_tag="ACHK74_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP0223211.1"
                     /translation="MSEVKKHVSYSLTVAAMLSASIVCQTAHADINTPNITGEKNNTT
                     TRFSGNIYGSENTVTSESNSLVVGNSNSVEGGYNNIVIGNSSTVKASGLIRKVGEDIV
                     LDGYSVSLGNATRINGDSSVAIGLTSSVTGNDSVALGSHSSANGNNIVSVGSDTLKRR
                     ITNLAQGKDDHDAVNLMQMSSAVNREAGERIKSTNNLQNQLNTLSGQVITETRARTES
                     DAATLAAARAHSDENDKRTLVSANTYADQKETDINNRTDGLLTTERQARTAGDTATLA
                     AARTHSDENDKRTLVSANTYADQKETAINNRTDGLLATERQARTAGDTATLAAARTHS
                     DKNNKRTLISANTYADQKETAINNRTDGLLTTERQARIAGDAATLATAAAYSDAGDSL
                     TLKQATAYSDISTRRALSEANRYTDNKFRNLSNKIERAEKRLNAGIAGVTAISSIPYV
                     AENSFSYGIGVGNYQNANAVAAGIQYKTSRNTNIRLNISWDSSHNTAIGAGVAGGW"
     gene            complement(5413..5487)
                     /locus_tag="ACHK74_004509"
     CDS             complement(5413..5487)
                     /locus_tag="ACHK74_004509"
                     /inference="COORDINATES: protein motif:HMM:NF013968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223212.1"
                     /translation="MLCFVWMVRDSLCGFTREIRGLWQ"
     gene            complement(6135..6377)
                     /locus_tag="ACHK74_004510"
     CDS             complement(6135..6377)
                     /locus_tag="ACHK74_004510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223213.1"
                     /translation="MMMARDITAYHLNSVHISARRFPEIFQWATGMQTKNLPLTNSLL
                     QNDYAESIFVFNGERDVSEMPLPEEILLTGNAESER"
     gene            6617..7894
                     /locus_tag="ACHK74_004511"
     CDS             6617..7894
                     /locus_tag="ACHK74_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP0223214.1"
                     /translation="MRKISVSYICKSLFLYCSASLPVLSANGIPDKMIVELGDNSAAK
                     ANFAVAVGRYSQANAEKSISLGASARAIENNSTALGANATTLKPFSVALGAGSVADRG
                     AGIEAYMPETKRWNPGDVVKSRMSDKQLNRLMEIDKIISAYNSQGTLSAEETKQLEEV
                     KKERADMFATYMATHDAVSVGVGATTTGRHAVTRQITSVAAGSVDTDAVNVAQLRDLQ
                     TVTEKADAALDKRITNNTSAITKNLQVVNSNKEEISNINKTTKILDERVTANTQTAEE
                     HKLLISENSKQISTNTLGIRENTQRIRDEGQARIRGDRETLRAATGYTDMRVNGLERN
                     TNKRFAQLKEDIDKNRKRADAGIAGAMAMTGIPSVPGKKVSFGMAMAGYRSEGAVAAG
                     FHFNTSENSAVKVNAAWDTENGAGVSAGMALGW"
     gene            complement(8324..8899)
                     /gene="dolP"
                     /locus_tag="ACHK74_004512"
     CDS             complement(8324..8899)
                     /gene="dolP"
                     /locus_tag="ACHK74_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFP0223215.1"
                     /translation="MRLLYSAVVLISALLLQGCVAATVVGTAAVGTKAATDPRSVGTQ
                     VDDEVLELRVSSALSKDEQIRKEARISEIAYQGRVLLIGQSPDRELSARARQIALGVD
                     GVQEVYNEIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLG
                     LVTEQEGRAAADIASRVSGVKRVTTAFSCIR"
     gene            complement(9245..9472)
                     /locus_tag="ACHK74_004513"
     CDS             complement(9245..9472)
                     /locus_tag="ACHK74_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HFP0223216.1"
                     /translation="MFPEYRELISALKGNHKRFDSLFEKHNKLDHEIKRIEDEFGSAY
                     STKVVRLKLEKLEIKREIQKILEDESEKGER"
     gene            complement(10144..11235)
                     /locus_tag="ACHK74_004514"
     CDS             complement(10144..11235)
                     /locus_tag="ACHK74_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP0223217.1"
                     /translation="MITCVFIFTAILLAVITFIPVSGNTSWVVRVWDFPRLQILLLCT
                     LNVFLSVVFLDSFAELFVVIISLICVAYQLVWIIPYTVIGKKQVKEIKDSDGFPGLSI
                     LVFNVLMTNKKYMPLLELINKEKPDILVLLETNLWWEQKLSPLDEVFPYSLKCPKENL
                     YGMHVYSRMILSDAEIQYLVDKEIPSMHMIVHMPEGQRIRLHCLHPVPPSPTESDEST
                     DRDSELLMVGKSVADSELPVILAGDLNDVAWSATTRLFMKISGLLDPRRGRGFFSTFH
                     SHYFFLRWPLDHVFHSQHFLLKSLRCLPDMGSDHFPVMAELVYAPAHGMQQESLEKEK
                     SDEVLAQKKMDMTDSTAEDVHTPGGQKKS"
BASE COUNT         3212 a         2922 c         2390 g         2809 t
ORIGIN      
        1 ttcaccatgt tctgctcttc cgcttctgca aacctctgca caaccaggta aaccctgagc
       61 atctgattgt cactcagtgt ctttttcttt ttttcagtac tcgcaactga tgcttccacc
      121 aacgggtttt ctgtctgagg cagcagtttc atcttgatcc cgaatcccat aattgcccgc
      181 aagtgggcca ccttgttatt ccaggtcacc tccctgcgct tctgctcttt caggacataa
      241 cgccggtatc gctgaacatc acggacagtc acctcccccg gcaaaatatc accgataaat
      301 tccctgaata ccctgacaac tttgcagtag ctccactctg tatccggccg aagattcttt
      361 gaaaaaaagt actcttccag cagttgttcc caggtgattt ttgtgacgga atcgtcattc
      421 tgcattatat attccccttg tttactgtgc tcccgcactc tccggtcaaa ttctgcacat
      481 catcgccttt actcacccga aaacaataag cgggctgtct ccgggaaccg taatattccc
      541 gtacagaaac ctgcccttaa ccagatatcc cgaaaccatt ttgtaacgtc cggaatgttc
      601 tgcgtcacta taaagacggg cctgaaaaaa acgctctcct gaacgtacct tatggatacc
      661 ttcccgttca aagagcgact gaacataacg acgatcctgc ccggaaccac tctccttcag
      721 aaaacgataa aaaatccccg gggcacacag aaaaacatat ccggaaacaa catgaacatt
      781 tgaatcaacg ctgttaaccg aaagtgaacc atccctcagc ccctctttta accatctgat
      841 aaattcactc tctaccctgc tatcctctgt cagggaaaac tctgccgttt tttccactgt
      901 ttccgggata cggtttccct gattttctgt tacacatccg ctctcttccg gtaactcaat
      961 accgctttct ttatccggcg gcacgcctga cgctatctgc cctccctcat ccgacaaatc
     1021 agcagccgaa aacagcgtaa ggagcattcc ggtatcagca tcagtttcgt tcccctcatc
     1081 cacctcctgt tccgctgtcc ggccctcatt ttcggcatcc tccactggcg actgtacaac
     1141 cggcaacggc tgaatgacgc tttctgctga aattgctgct gactgcagca gtaccggaac
     1201 aacctcagcc accggaactt cggccacatc tcctgcagcg aagggactca cgacttcctc
     1261 catagcttcc tgtaatactg tacgtatcag cgccatttca ggatgctgat gccagactgc
     1321 tccggcaaga ttctgtaatg cccccgggac agtggcaagc caggctcccg cctctccggg
     1381 cagcagttgt cctgccatca gggctgccag ttgccgtccc tccgttccct ggcagggcat
     1441 cttcctgaac cggaaccggt atacgccctc cggtacagat atccccggct gccagcatct
     1501 gccgtcggcc tgttcgcctt ccagttccgc cagcaacggc aggtgataaa acagagccga
     1561 ccagaacacc accatgttcc acacactccc ctgcgcggcc tgctcttctg ctgtcgctcc
     1621 cggtggaaac attctgcctc tggccagccg gacagcatac gtcgtgaact gcagggtcag
     1681 gtcaccaaaa ccaccggtgt aaccccagcg accttctgct gtggccggca gattctgact
     1741 gacagacaac aactgatgca caggatcaag acaaaggcgc tgatacatat ccaccggcat
     1801 ggaactgttt tcccataact gtttcagaca ccttttcctg aacggtgtgg ataccagtac
     1861 atcaaccggc aatggtgtaa aatagccctc cggcaccgtt tccccgggaa tatccgtccg
     1921 gacatcagaa cattctgtct tcacaggaaa taaccttttc aggcgttcaa gcatgcccat
     1981 tctcctgaac ccgacctgaa gtgtcgtacc cggtcccccg cagaccttca gttttctgct
     2041 gttcttctgc cagttgatgc atcagctcat tcagtaccag cgtcctgagg gcccgggtga
     2101 cagccacata taacagacag gtttcatcat gacgctcagc ctccgtcatc agcggatccg
     2161 tgatatcctg gaaatcctca tccagcatta caaccggcca ctccagcccc ttgctccggt
     2221 gtgccgttga caccgtgata tctgcatcct gctcattcgt taccgtatac tgccggagaa
     2281 gttccagttt tttcagtaac ggaaaacaca actccagaag ccgaagggac tgattcatct
     2341 ccacatcctt cgtggatttt gcaatatatt tatattcttc aaaattcctg tagtcccggc
     2401 gcagcctgtc tgaatgcatc cgttccggca tatcaacctg gaaccagtag agatcctcaa
     2461 gttccccggt cctgtaacct tcgattcccc ccacccagta aaccttttgc cctttcattg
     2521 ccgccatcag cgcagtagca atcacacccg cgacagtacg actgagtacc gtgtaatgcc
     2581 cctgcagatg ttcacgcgca tggcatttca gcaatacctc atcatcacca ccacaacctt
     2641 tcacctctcg tgtttcacct tcacgctgca gaagcagatt tgccatacga gccaccgccg
     2701 gaccaaaacg gaaactctgt gtcagccaca accggtccgc attcttcagt gcaggatggg
     2761 aaagcgcatt attcgcaccc cgaaagcggt aaatctgttg ataacaatct ccaacaagga
     2821 ccacccgaca cttctgcccc agaaccagag cagtggtcac cggattcgca tcctgcgcct
     2881 catcaaacag gatggtgtcc catcggtgag acaggtctgc accggacaac tggaagagtt
     2941 tcagataagt atcgtgcgtc accgggaacg tccccccggg gttgctcatt tcgttccaga
     3001 gcaactgcac cgcccgaagc gcatctgctg gcggaacacc ccgcatctca tccttatccg
     3061 gcaggtgatg caggcccggt accgcatcgg cagaacagat aaagtgattc agccccgtcc
     3121 ctgccacccg tgccagagcc cagtatcgtg tattcagctt tctcgccaca tcagtaatac
     3181 gtaatcccgg aaccagacga tgacggtagt gtcttccaac ccgggcatac gccagctgat
     3241 gtgacgttct gcattccacg ttaaagggaa atttacgctc agcctcatca cgtattgccc
     3301 ggttataggc aagatagagc atacggctgt ccggactggc ccgggcaaac tgcaccagcg
     3361 tactcgtctt ccctgtgccg gaaaacgcat tcacaaccag ttgctgcccc tgccagttaa
     3421 taatggcgta ctgttcatct gtgggattaa aagacatgca ttctcttctc cggaaaaaca
     3481 tttccgtaat ctgatgcatc aggggtatgc ccaccacaaa taactctgta cagcaacaga
     3541 aaaatttccc tggtcatatg atgaattaat catcgccatc acaccggata cttctcatcc
     3601 tgtcctcaga aaggcaggtg gtctcctgtc tgccgcgctt atccaggata ccgggacctg
     3661 aaaggtcata atctggcagg aaaaaataat gtccgaagta aaaaaacacg tttcttattc
     3721 attaacagtt gcagctatgc tgtcagcctc catcgtctgt cagacagcac atgctgatat
     3781 caacacaccg aacatcaccg gagaaaaaaa caatacaaca acacgattct ccggaaatat
     3841 ttacggcagt gaaaatactg tcacatcaga gtcaaacagt ctggttgtgg gtaacagtaa
     3901 ttccgtggag ggagggtata acaacattgt tattggtaat tcctccacgg tcaaagcttc
     3961 cggtttgatc agaaaagtgg gcgaagatat tgtgctggat ggttacagcg tcagcctcgg
     4021 taatgccacc agaattaacg gtgattcatc tgttgccata ggtctgacat cgtccgtaac
     4081 cgggaacgac tccgtggcac tgggcagtca ttccagtgct aacggaaaca atatcgtatc
     4141 cgtcggctct gatacactga aacgccgcat cacaaatctg gcacagggga aagatgacca
     4201 tgatgcggta aatctgatgc agatgagcag tgctgttaac cgggaagctg gagaaagaat
     4261 aaaatcaaca aacaacctgc agaatcagct taataccctc tctggtcagg tcataactga
     4321 aaccagagcc cgaacagaaa gtgatgcagc aacgctggct gcagccagag cccacagcga
     4381 tgaaaacgac aaaagaacac tggtcagtgc gaatacctat gctgaccaga aagaaaccga
     4441 catcaataac cgtactgacg gattactgac gaccgaacgc caggcacgca ctgcgggcga
     4501 tactgcaact ctggctgctg ccagaacgca cagcgatgaa aacgacaaaa gaacactggt
     4561 cagtgcaaat acttatgctg accagaaaga aaccgccatc aataaccgta ctgacggatt
     4621 actggctaca gaacgccagg cacgcactgc gggcgatact gcaactctgg ctgctgccag
     4681 aacgcacagc gataaaaaca acaaaagaac actgatcagt gcgaatacct atgctgacca
     4741 gaaagaaacc gccatcaata accgtactga cggattactg acgaccgaac gccaggcacg
     4801 catcgcaggt gatgccgcaa ctctggcaac agcagcggca tacagtgacg ccggggattc
     4861 actgacactg aaacaggcaa cagcctacag cgatatcagt acccgccggg cactgtcaga
     4921 ggccaatcgt tataccgaca acaaattcag aaacctgagc aataaaattg aacgggcaga
     4981 aaaaagactt aatgcaggca ttgccggagt gacagcaata tcctccatcc cctatgtggc
     5041 tgaaaattct ttttcatacg ggatcggcgt gggtaactat cagaacgcta atgctgtggc
     5101 cgcaggcatt cagtataaaa catcacgtaa taccaatata cgcctcaaca tctcctggga
     5161 ctcatctcat aacaccgcga ttggtgcagg ggttgccggc ggctggtaaa taatcttgcg
     5221 tacatacggc attatggtat tccgtaatca cattaattca gtacgcaaca cagccccatc
     5281 cggaacagac cactggactg agcgggataa tcaaggaaaa aggacgcgca gtgtggtcag
     5341 ccttcacaac aatccggcgg cagatttcct gccgccatgt tgcccgtaaa catttaagtt
     5401 cgcaggctaa cattattgcc acaagccccg tatttccctg gtgaatccgc aaagcgagtc
     5461 cctcaccatc cagacaaaac acagtatcgt cgattaataa tcactaaaag cgttcgtcgt
     5521 agcatcataa cccctgaatg aacacatccc tgtttgcaag gattctgttg ttctgatatc
     5581 gacgagtagc tgcaactctg tatccgccct tattgcagtc agtgcagtca ggccacgatg
     5641 cgattcgctg acctgagcct tattctccag gcggacttta tgcaatgctc ccctgaaatc
     5701 ttccattatt ttacatgtac aggcatcata ttaaatatgg aaataataac gaacggaaaa
     5761 gggacagaat gaatatcaaa gcaccagtat aaaccaggat gttttgagca atttcttact
     5821 ctcctgaata cacactgtaa atcatcagaa actgcactag ccatgctaag tacactctgc
     5881 tacgaactga tgaggaagtg cggtatcaat tacttggaca gcctgatcaa cggagaggta
     5941 tatctattat ctatgtaatt acagggggac tgatatcagc gaaaaagcag gtcagcatat
     6001 tcactgcccc tggtctgaac tgaaacaacg tcagccagaa ataatcctat aatgcctcat
     6061 ataagtggat aacttttcat ccgtcgttga catcatacac atgaaacatt gataacagtc
     6121 atgaatacac cgaattatct ttctgactca gcgtttccgg tcagtagtat ttcttccggt
     6181 aaaggcattt ccgaaacgtc acgttcgcca ttaaatacaa aaatactttc tgcgtaatca
     6241 ttctgtaaca aagaattagt taacggcaga ttttttgttt gcataccggt ggcccactga
     6301 aaaatctcag ggaaacgacg agcactgatg tgtacagaat tgaggtggta cgcagtaata
     6361 tctcttgcca tcatcataag atggcacatc atatcccgaa cgcaaacctg gcacatgatg
     6421 aacagacgga aggctctgca ggacttctgg ataattatac acgatagcca gttactgctg
     6481 acctggattt attatcatac cacattattt atttcaaaaa ataaatttta aattatttat
     6541 ttttacttgt atataaatat ccaacgatgg ttctgtttgt attatgtata ctatataaaa
     6601 gagatggtga attcaaatgc gtaaaatcag tgtcagttat atatgcaaat cattatttct
     6661 ctactgctca gcttctttac ctgtcttatc tgcaaatggt attcctgata aaatgatcgt
     6721 ggaacttggt gataacagtg cggcaaaagc taatttcgct gttgctgttg gtcgatattc
     6781 acaggcaaat gcagaaaaaa gtatttcttt aggtgcatca gcaagagcta ttgaaaataa
     6841 ctctaccgcg ttaggagcaa acgcaacaac attgaaacca ttctctgtcg ctctgggcgc
     6901 aggaagtgtt gctgatcgtg gcgctggtat tgaagcatat atgccagaga caaagcgctg
     6961 gaatccaggg gatgtagtaa aatccaggat gtctgacaag cagcttaaca ggcttatgga
     7021 aatcgataag ataatatccg cttataattc acaagggacg ctgtctgctg aagagactaa
     7081 acaactggag gaagtgaaaa aagagcgggc tgatatgttt gcgacatata tggcaacgca
     7141 cgatgctgta tctgtaggtg ttggtgcaac gacaacagga cgacatgctg ttacaagaca
     7201 gataactagc gttgcagcag gttctgtcga tacggatgct gttaacgttg cacagctcag
     7261 ggatttgcag actgtgacag aaaaggcaga tgcagcgctt gataaacgca taacaaacaa
     7321 caccagtgcg ataactaaaa atctgcaggt ggttaacagt aataaagagg aaatcagtaa
     7381 cattaataaa acgacaaaaa ttctggatga aagagtgaca gccaatacac agacagcaga
     7441 agaacataag ctgcttattt cggagaacag caaacaaatc agtaccaaca ctcttggtat
     7501 cagagagaat acacaacgga tccgggatga aggacaggct cgaatccggg gagacaggga
     7561 aacactgcgt gcagcgaccg gttataccga catgcgggta aacgggctgg agcgtaatac
     7621 gaataaacgt tttgcgcagc ttaaagagga tatagacaaa aacagaaaac gtgcagatgc
     7681 aggtattgca ggtgcgatgg caatgacggg gataccgtcc gtacccggga aaaaggtctc
     7741 atttggtatg gccatggccg gataccggag tgaaggtgct gttgctgccg gcttccattt
     7801 taatacaagt gaaaacagtg ctgttaaagt gaatgcagcc tgggacactg aaaatggagc
     7861 tggcgtttca gccgggatgg ctctgggatg gtaatgcttc cgggccagaa gccgttcgca
     7921 gatattgtta tccaggggac actgcaccac cattcaggaa gcgattcagt ttatcgcatc
     7981 ggctcaggat gtaattaata cggacaatct ggctccagca ccggagccag acgtacacgt
     8041 gtcaacgcca cgctctttga cctgtatgga taaaagctca gattatttac cagcagcgac
     8101 atccggagag caccgggatt ttatctgtga gtgaacatac ccgggcaaaa aaatcccccg
     8161 aattcagggg ggtgtcaaag aggaattatg tatattggtt attataatga gcacgtctcc
     8221 ggtattccgc gacgaatgtt cacgtggtgg catgaacggc ccggaaacgt gctcttctga
     8281 tttttaacgt aaatttgcac taaaggcaac tcagatacgg ccttcaccgt atacaggaga
     8341 atgccgtcgt gacccgcttc acgccactca ccctactggc aatatctgct gccgctcgcc
     8401 cttcctgctc tgtaaccagg cccagcaaaa agacttcgct gttttctgtc accaccttga
     8461 cactggatga tttgacacgg gcacttgtga gtaactgaga acgaattttt gtcgtgagcc
     8521 aggcatcacc agccattatg accggactga cgggagtccc ctgccggatt tcattataga
     8581 cctcctgaac accatcgaca ccgagtgcta tctgacgggc acgggcagat aattcacggt
     8641 ccggagactg accgatcagc agcacgcgtc cctgatatgc aatctcactg atgcgtgctt
     8701 cttttctgat ttgttcatct tttgaaagtg cactgctgac cctcagctcc agcacttcat
     8761 catccacctg cgttccgaca ctgcgtgggt cggttgctgc ctttgtcccg acagcggctg
     8821 tacccaccac agttgcagca acgcatccct gtaacaataa ggctgaaatc aggacaacag
     8881 cagaatataa taaacgcata tctcccccgg aatttatcgt acaaaaagtt taccttcagt
     8941 gaaacaaaac aagctacgga ctgttattct cttccggcac agccggaaac agcggatacg
     9001 ttacgtgaca tatctgtatc tgccagacgg aaatgtttat gacggatatg gatgtactga
     9061 aatttaatat tgttcatgtt tttgcacgaa aacgttgata gcgtagtaaa gattctgttt
     9121 tttaactacc tggtttgctg aacaggatct ccttttcgtt atacacaggc taaaaggcac
     9181 cgtggccccc cggtgccttt ttcataaaac agagaaaaca cggctttgaa tataccgttt
     9241 atgattaccg ttcgcccttt tctgactcat cttcaaggat tttctggatt tctcttttga
     9301 tctcaagttt ttcaagcttc agcctgacca ctttcgtcga atacgctgaa ccaaattcat
     9361 cttcaattct ttttatttcg tgatcaagtt tattatgctt ttcaaataaa gaatcaaagc
     9421 gtttatgatt tcctttcaat gctgaaatca gttctctgta ctccgggaac attgttttta
     9481 tctcctttca tcaacactgt aacaatacat ataaacttta aatttattaa agattctcac
     9541 catcattcgc agtgaataat gccgcatctc atattcatat cctgcagaga aaacccctcc
     9601 gaagaggggc gagtgtagcg ttaatggagt gttatgtagc atggtgccgg gtgcctcccg
     9661 gtgaacagac tgttcgtgat accagtcagc aacacaggaa attaatggta tcaccccacc
     9721 gcacaggggg attcaccatg cctgcctttt ttagcaaatt catcagaaac cggacaacat
     9781 gctcatttat gcaatgaggt gcttttcaca tttatataaa atcattataa cgataatcct
     9841 aaaaatcaat gtaattatca tcttcagtaa ctttctgtat cacgccatac ggaaattgat
     9901 ttttgacggt aaagatacaa ggggaagtat gtctgtaagg agtaccctga ataatggaca
     9961 ggcatgcatc gtggctgttc ccctgaaatt atcagtacaa ccagtcagct cataatccct
    10021 gtctcttttt ccggggagcc ctcaggggga ctttccctta tccccccagt ttctggagag
    10081 acgtaaaaaa gaattaatca tatgtcaggt atctgaatat gtcccgtaat taatatattt
    10141 acgtcaggat tttttctgcc cgccaggagt atgaacatct tccgccgttg agtctgtcat
    10201 atccattttt ttctgtgcca gtacttcatc tgatttttct ttttccagac tctcctgctg
    10261 catcccgtga gccggagcat atacaagctc agccatcact ggaaaatgat ctgaacccat
    10321 atccggtaaa cagcgtaaac ttttaagcag aaaatgctgg ctgtgaaata catggtcaag
    10381 cggccatcgc aggaaaaaat aatggctgtg aaacgtgcta aaaaaaccac gtccccggcg
    10441 tggatccagc agtccactta ttttcatgaa taaacgagtg gtcgcagacc acgcaacatc
    10501 attcagatcc cccgctaata ttacaggtaa ttcagaatct gcaacacttt tcccgaccat
    10561 taaaagttct gagtcacgat cagttgactc atcactttca gtcgggctcg gtggaacagg
    10621 atgaagacaa tgtaatctta ttctctgacc ttccggcata tgaactatca tatgcatgga
    10681 gggaatttcc ttgtccacta aatactgtat ttctgcatca cttaaaatca tccttgaata
    10741 tacatgcatg ccatataaat tttctttcgg gcatttcagg gagtatggaa acacttcatc
    10801 cagaggtgat agtttctgtt cccaccataa gtttgtttca agaagaacaa gtatatcagg
    10861 tttttcttta tttataagtt caagcaaagg catatatttt ttattggtca taaggacatt
    10921 gaacaccagt atacttaaac ccggaaatcc atcagagtcc tttatttcct ttacctgctt
    10981 cttgccaatt actgtataag gaattatcca taccaactgg taagcgacac aaattaaact
    11041 tattatcaca acaaacaact ctgcaaatga gtcaagaaaa acaacagaca gaaaaacatt
    11101 caaagtacac agcaaaagta tctgtagtcg gggaaaatcc cataccctga caacccatga
    11161 tgtattaccg gaaacaggga taaaagttat gactgccaga aggatagcag taaaaataaa
    11221 aacacaagtt atcacaaatc actccttgtt ctgaaccggc acacaaaact gtcatatacg
    11281 tttcaaaagt aaaaacacac tgctgccaca agattcacga cgtaaccggg cag
//
LOCUS       DAZGSP010000141        11324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZGSP010000141 DAZGSP010000000
VERSION     DAZGSP010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.142"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            90..224
                     /locus_tag="ACHK74_004515"
     CDS             90..224
                     /locus_tag="ACHK74_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFP0223218.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            203..307
                     /gene="speFL"
                     /locus_tag="ACHK74_004516"
     CDS             203..307
                     /gene="speFL"
                     /locus_tag="ACHK74_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFP0223219.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            618..2816
                     /gene="speF"
                     /locus_tag="ACHK74_004517"
     CDS             618..2816
                     /gene="speF"
                     /locus_tag="ACHK74_004517"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFP0223220.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2813..4132
                     /gene="potE"
                     /locus_tag="ACHK74_004518"
     CDS             2813..4132
                     /gene="potE"
                     /locus_tag="ACHK74_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFP0223221.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4188..5828)
                     /gene="pgm"
                     /locus_tag="ACHK74_004519"
     CDS             complement(4188..5828)
                     /gene="pgm"
                     /locus_tag="ACHK74_004519"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFP0223222.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILLHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5854..6399)
                     /gene="seqA"
                     /locus_tag="ACHK74_004520"
     CDS             complement(5854..6399)
                     /gene="seqA"
                     /locus_tag="ACHK74_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFP0223223.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSASSQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVRGTI"
     gene            6584..7348
                     /gene="ybfF"
                     /locus_tag="ACHK74_004521"
     CDS             6584..7348
                     /gene="ybfF"
                     /locus_tag="ACHK74_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP0223224.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7488..7781
                     /gene="ybfE"
                     /locus_tag="ACHK74_004522"
     CDS             7488..7781
                     /gene="ybfE"
                     /locus_tag="ACHK74_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFP0223225.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7921..8451
                     /gene="fldA"
                     /locus_tag="ACHK74_004523"
     CDS             7921..8451
                     /gene="fldA"
                     /locus_tag="ACHK74_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFP0223226.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8661..8747
                     /gene="uof"
                     /locus_tag="ACHK74_004524"
     CDS             8661..8747
                     /gene="uof"
                     /locus_tag="ACHK74_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFP0223227.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8740..9186
                     /gene="fur"
                     /locus_tag="ACHK74_004525"
     CDS             8740..9186
                     /gene="fur"
                     /locus_tag="ACHK74_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFP0223228.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9270..9596)
                     /gene="chiQ"
                     /locus_tag="ACHK74_004526"
     CDS             complement(9270..9596)
                     /gene="chiQ"
                     /locus_tag="ACHK74_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFP0223229.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9646..11052)
                     /gene="chiP"
                     /locus_tag="ACHK74_004527"
     CDS             complement(9646..11052)
                     /gene="chiP"
                     /locus_tag="ACHK74_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFP0223230.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2762 a         2781 c         2809 g         2972 t
ORIGIN      
        1 caggtttaat ttcttaacgc aaatataaaa cacaaaatta caacatttaa ataacaaaac
       61 agcatctaat atggcgctat tcataattaa tgatttatta tttggggtat gatcgttttt
      121 tgttgatctt cttcacagat tatagccatt tcatggatag aataactcta ccttcaactg
      181 acacagcaag aggtaaaggt aaatggaaaa taataaccgc ttaatgcctc atataaggcg
      241 gacaacccat atcatgaagt ttgcccatcg taatagtttt gactttcatt tctttaatgc
      301 ccgctagtct tctgactaaa gggcacccca acgtacaggt ctccctgact ttaagcatta
      361 caggttaata cctgtattcc tcggtgctca tatactgcta acccttttaa actctaaata
      421 attcgagtcg cagcacttgc aacttgaggt atgacgagta tagccagtta ccgggctggt
      481 ctgggttatt gcatctgcaa aaagcaaact actgatttat ttatcagcgg tggagctttg
      541 ctttttttcc ggcgtgatcg atttctcctt tgagaaattg aggacctgct attacctgaa
      601 ataaagagat gaacaaaatg tcagaattaa aaattgccgt tagtcgttct tgcccggatt
      661 gtttttccac tcatcgtgca tgcgtgaata tagacgaaag taattatatt gacgttgccg
      721 ccattatttt atcagtcagt gatgttgaac gtggaaaact cgatgaaata gacgctactg
      781 gctatgacat tcctgttttt attgcaacgg aaaatgaaga acgtgtaccc gcagaatatc
      841 tgccacgtat ttctggtgtc ttcgaacatt gcgaatcacg taaagagttt tatggacgtc
      901 agttagaaac cgctgccagc cattatgaaa cgcaactgcg cccgcccttc ttccgcgcac
      961 tggtcgatta tgttaatcag ggcaatagtg cttttgactg ccctggtcat caggggggtg
     1021 agtttttccg tcgccatccg gcaggcaatc agttcgtgga atactttggc gaaatgctct
     1081 tccgctctga cttgtgtaat gctgacgtag caatgggcga tctgctgatt catgaaggtg
     1141 cgccatgtat tgcacaacag catgcggcta aagtctttaa tgccgataaa acctatttcg
     1201 ttttaaacgg tacttcatct tctaataaag tggtcttaaa cgcattgctg acgccgggcg
     1261 atctggtgtt gtttgaccgc aataaccata aatctaacca ccacggtgcg ttgctacagg
     1321 ctggcgcgac accggtttat ctggaaaccg cacgtaaccc gtatggtttt atcggtggta
     1381 ttgacgctca ctgttttgaa gaaagttacc tgcgtgagct gattacagaa gttgcaccgc
     1441 agcgggcaaa agaggctcgt cctttccgcc tcgccgttat tcagctaggc acttacgacg
     1501 gtacgattta taacgcccgt caggtggtgg ataaaattgg tcatctgtgt gactacatcc
     1561 tctttgactc cgcatgggtc ggctatgaac agtttattcc gatgatggct gactgttcgc
     1621 cgctgttgct ggatcttaat gagaacgatc cgggtattct ggttacgcaa tccgtacata
     1681 aacagcaggc tggtttttct caaacttcac aaatccataa aaaagacagc cacatcaaag
     1741 ggcaacagcg ttatgtaccg cacaaacgca tgaacaacgc ctttatgatg cacgcctcca
     1801 ccagcccgtt ctatccgctg tttgccgcac tggatatcaa cgccaaaatg catgaaggtg
     1861 tcagcggtcg taatatgtgg atggattgtg tggtaaatgg cattaatgcc cgcaaactga
     1921 tcctcgataa ctgtcagcat attcgtccgt tcgtacctga actggtggat ggtaaaccct
     1981 ggcagtcgta tgaaacagcg caaattgcgg ttgatctgcg cttcttccag tttgtaccag
     2041 gggaacactg gcattctttt gaaggctatg cagagaatca atactttgtc gatccttgca
     2101 aactgttgct gacaacccca ggtattgatg cacgtaacgg cgaatatgaa gcgttcggtg
     2161 tacccgcgac gattcttgct aacttcctgc gcgaaaatgg cgtagtgccg gaaaaatgcg
     2221 atcttaactc catcctcttc ctgctgactc cggcagaaga tatggccaag cttcagcaac
     2281 ttgttgccct gctggtacgc ttcgaaaaac tgcttgagtc cgacgcgcca ttagcagaag
     2341 tgctgccttc catctacaaa cagcatgaag agcgctacgc cggttatacc ctgcgtcagt
     2401 tgtgtcaaga aatgcatgat ttgtatgccc gtcacaacgt gaaacaactg caaaaagaga
     2461 tgttccgtaa ggaacacttc ccacgcgtaa gcatgaatcc gcaagaagcc aactacgcct
     2521 atttacgcgg tgaagtggag ctggttcgtc tgccggatgc agaaggccgt atcgctgccg
     2581 aaggtgcgct tccttatcct ccgggtgtgc tgtgcgttgt tccgggtgaa atctggggtg
     2641 gtgctgttct gcgttacttc agcgctctgg aagaagggat caacctgttg ccaggttttg
     2701 caccggagct tcagggtgtc tatatcgaag aacatgatgg tcgtaagcaa gtttggtgct
     2761 atgtcatcaa gcctcgtgat gcgcaaagca ccctgttgaa aggggaaaaa ttatgagtca
     2821 ggctaaatcg aacaaaatgg gcgtcgttca gttaaccata ctgacgatgg tcaacatgat
     2881 gggctccggt atcatcatgc tgccgacaaa gcttgccgaa gttgggacaa tttcaattat
     2941 ctcctggctg gtgacagccg taggctcaat ggcactggca tgggcattcg caaaatgcgg
     3001 tatgttcagc cgtaaatcag gtggtatggg cggttatgcc gagtatgctt tcggtaagtc
     3061 cggcaatttt atggcgaact acacctacgg tgtctcgctg ctgattgcta acgtcgcgat
     3121 tgctatttcg gcggttggtt acggcaccga actgctcggc gcgagtttgt cgccagtgca
     3181 gattggtctt gcgaccatcg gagtgctgtg gatttgtacc gtggctaact ttggtggtgc
     3241 gcgcattacc gggcaaatca gtagcattac tgtgtggggg gtcattattc cggtggtagg
     3301 cctgtgcatt atcggctggt tctggtttag cccgacgctt tacgttgatt cctggaatcc
     3361 gcatcatgca ccgttcttca gtgcagtagg ttcttccatc gctatgacgc tgtgggcttt
     3421 tctcggtctg gagtctgcgt gtgcgaatac tgatgtagtg gaaaacccgg aacgtaatgt
     3481 gccaatcgcg gtactcggcg gtacgttagg tgcggcggtg atttatatcg tctccaccaa
     3541 cgtgattgca gggattgtgc ctaatatgga gctggcaaat tcaacggcac catttggcct
     3601 ggccttcgca cagatgttca caccagaagt gggtaaagtc attatggcgc tgatggtgat
     3661 gtcctgctgc ggttcgctgc ttggctggca gttcaccatt gcccaggtgt ttaaatcttc
     3721 atctgatgaa ggctacttcc ctaaaatttt ctcccgtgta accaaagtgg atgcaccggt
     3781 gcagggaatg ttgaccattg tgattatcca gagcgggttg gcgctgatga cgattagccc
     3841 gtcgctaaac agtcagttca acgtgctggt taacctggcc gtggtgacca atatcattcc
     3901 gtatattctg tcaatggcgg cattagtcat tattcagaag gtcgctaatg tgcccccctc
     3961 aaaagcgaaa gttgcaaact ttgttgcttt tgttggcgcg atgtatagct tttatgcgct
     4021 gtactcatcc ggggaagaag ccatgctgta cggttccatc gtgaccttcc ttggttggac
     4081 actgtatggt ctggtatcac cacgctttga actgaaaaat aaacacggtt aaaaaagcaa
     4141 agtaaaaaag ggcgatcttg cgaccgccct ttttttatta aatgtgttta cgcgtttttc
     4201 agaacttcgc taacaatctc aaccgcttct ttctcaatct gcttgcgatg ttcttcaccg
     4261 aggaagcttt cgcagtagat cttatatgcg tcttccgtgc ctgacggacg cgcggcgaac
     4321 cagccgttgt cagtcatcac tttcagaccg ccgatagaag caccattgcc cggtgccgcc
     4381 gtcaggcgcg cggtgatcgg gtcgcctgcc agggtgctgg cgctcaccat ttccggagac
     4441 agcttagaca gcgccgcttt ttgtgcggaa gtcgcagctg cctgcaaacg gttgtagctc
     4501 ggcgcaccaa agcgttttgc cagttcgttg tagtgttcct gcgggttctt accggtgacc
     4561 gcggtgattt ccgccgccag cagacacatg atgatgccgt ctttgtcggt ggaccacggc
     4621 gtgccgtcga aacgcaggaa ggaagcccct gcgctctctt cgccgccaaa accgaagctg
     4681 ccgtcgaaca gaccatcaac aaaccatttg aaacctaccg gtacttctac cagcttacgg
     4741 cccaagtcat tgaccacacg gtcgatcatc gcagaagaaa ccagcgtttt accgacagca
     4801 acatctttgc cccactgcgg acgatgctgg aacaggtagt tgatcgccac cgccaggtag
     4861 tggttcggat tcatcaaacc tgccggagtg acgataccgt gacggtcata atccgggtcg
     4921 ttagcaaacg ccagatcgaa cttatcacgc agtgccagca ggcccgccat cgcacactcg
     4981 gaggagcagt ccatacggat cgcgccgtct ttatcaaggt gcataaagcg gaaggtttga
     5041 tcgacctgat cgttaacgat ggtcaggttg aggttgtaat actcgccaat acgcttccag
     5101 tattcgatac cggaaccgcc cagcggatca acgcccagcg tcaggcccgc tttctgaatt
     5161 gccgccatat caacgatatc ggccagacct tccacaaacg gctgcaccag atcctgctct
     5221 tttacatgac cggatgccat cgcttcgtcg agggagatac gcttcacgcc tttcaggcca
     5281 tcggccatca gtgcgttggc cctgtcttcc accactttgg tgacgttagt atcagccggg
     5341 ccaccatttg gcggattgta cttgatacca ccatcttccg gcgggttatg ggacggtgta
     5401 atcacgatac cgtctgccag cgggccacct tttttattgt gaagcaggat ggcattggaa
     5461 acggcaggcg tcggagtgaa gccattgttt tcctgcacaa tgacatcaac gccgttcgct
     5521 gccagcactt ccagtacgga aatgaacgca ggctcggaca gggcgtgagt atctttaccc
     5581 acatagcaag ggccagtgat gccatttttc gcacgttctt cagcaattgc ctgagcgatc
     5641 gccagaatgt gcggctcgtt aaagctgtgg cgcgctgcac tgccacggtg accggaagta
     5701 ccgaatttca ccgcgtgctc cgcattccct gcttctggtt tcagtacata atattgcgcc
     5761 gtcagttggg cgacgttaat caaatcactc tgttgtgcag gttggcctgc acgattgtgg
     5821 attgccattg ctttgtcctt tgtctgcaac gttttagata gttccgcgaa ccttctcaat
     5881 caattccgcc gggaattgca tcgactgcat gatgtgttcg atcatgctgc atttacggcc
     5941 ggtgttggtg ttggtgatca cccaatacgg cgtgcctggc acatgtttcg gcttggtctg
     6001 attaccattt ttcagcagcg tttgttcatc tgccgcaaag taaacgcgtg tacgaccgtg
     6061 caacgattcc gttgcttcgg caaacgcctg ggcgtcaaga gaatatagtg tagacaacag
     6121 cagcataaag cgattgaccg ctcgcttttg ctctgcgtat tcgtccgaaa gcagaagttc
     6181 acgcattgcg cgaaccttgt ctttaatcgt tttgaccggc ttcgcttcga cgatagcagg
     6241 tgacgcaacg cgaacctctt tcgtcaccgg agcagcaggc tgtgatgagg cggaaaattt
     6301 caacatacgc cgtaaaatgt cggatgcgct ctcgccgata tgcttagtgt ggctggcaat
     6361 atagctgtag agttcatcat caacttcaat cgttttcatc ttaatccagt gcagtgtctt
     6421 agctgaatac aagtcgccag gagtatatga ataaatcccg gcagcggata gcgtcaagcc
     6481 tggcttgcag aaaaactcgg aataaacctt atttgttgca gccctgtggc acaatggtca
     6541 acataatacc ctaacccgac aacgcgaaaa aaagaacttt gccatgaaat tgaatatccg
     6601 cgcgcaaact gcacaaaacc agcacaataa ttctcccatc gttcttgtcc atggtctgtt
     6661 tggtagcctc gacaaccttg gcgtactggc tcgcgatctg gtaaacgatc acaatatcat
     6721 ccaggttgat atgcgtaacc acggtctttc accgagagat ccggtaatga attacccggc
     6781 gatggcgcag gatcttgttg atactctgga tgcacagcag atcgacaaag caacatttat
     6841 cggtcactcc atgggcggta aagcggtaat ggcacttact gcactggccc ccgatcgcat
     6901 cgataaactg gtggcgatcg atatcgcacc ggtcgactat cacgtacgcc gtcatgatga
     6961 gattttcgcg gctatcaacg cggtcagtga atcggacgca caaactcgcc agcaagcagc
     7021 agcaataatg cgccagcatc ttaatgaaga aggggtgatt cagtttctgt tgaaatcttt
     7081 tgttgacggg gagtggcgct ttaacgtgcc ggtattgtgg gatcagtatc cgcatattgt
     7141 gggttgggag aaaatcccgg catgggatca ccctgccctg tttatccctg gcggcaattc
     7201 tccgtatgtt agcgagcagt accgtgatga tttactggca caatttccac aggcacgggc
     7261 gcatgtgatt gcaggcgcgg gtcactgggt ccatgctgaa aaaccggatg cggtattacg
     7321 cgctatccgt cgctatctca atgattaacg caactgatta aaaacccagc gtccgcgtcg
     7381 ctttccgcgc gtgggcgttg gcgtgactca tcgcgctgat gtattatggc gcgctatcca
     7441 tccgggcaga ggcctggctg attgtttccc ccgaagtcac caagatcatg gccaaagaac
     7501 aaacggaccg tacgacatta gatctgttcg cgcacgagcg tcgaccggga cgaccgaaaa
     7561 ctaatccgct ttcgcgcgat gaacagctgc gtattaataa acgcaaccag ctaaaacgcg
     7621 acaaagtacg tggccttaag cgtgtcgaac tgaagctgaa cgcggaagct gtcgaggcgc
     7681 tgaacgagct ggcggagtcg cgcaatatga gccgtagcga actgatcgaa gagatgttaa
     7741 tgcagcaact ggcggcgctg cgtagtcagg gcatcgttta aatttccact ttcatgtagc
     7801 acagtgtgca gtcctgctcg tttgctgatt ccgcaagact gcctgttctg ctatgattgc
     7861 ctttatccgt gggcaatttt ccacccccat ttcaataagt ttcaagaggt tatttcactc
     7921 atggctatca ctggcatctt tttcggcagc gacaccggta ataccgaaaa tatcgcaaaa
     7981 atgattcaaa aacagcttgg taaagacgtt gccgatgtcc atgacattgc aaaaagcagc
     8041 aaagaagatc tggaagctta tgacattctg ctgctgggca tcccaacctg gtattacggc
     8101 gaagcgcagt gtgactggga tgacttcttc ccgactctcg aagagattga tttcaacggc
     8161 aaactggttg cgctgtttgg ttgtggtgac caggaagatt acgccgaata tttctgcgac
     8221 gcattgggca ccatccgcga catcattgaa ccgcgcggtg caaccatcgt tggtcactgg
     8281 ccaactgcgg gctatcattt cgaagcatca aaaggtctgg cagatgacga ccactttgtc
     8341 ggtctggcta tcgacgaaga ccgtcagccg gaactgaccg ctgaacgtgt agaaaaatgg
     8401 gttaaacaga tttctgaaga gttgcatctc gacgaaattc ttaatgcctg atgtgatgcg
     8461 gcgtagactc atgtctacgc cgtattaata gataatgcca atcaaaataa ttgctacaaa
     8521 tttgtaactt ttgctgttgt acctgtacaa tgtcccggtg ttcaagtggc cttgccgttg
     8581 taaatgtaag ctgtgccacg tttttattaa caatatttgc cagggacttg tggttttcat
     8641 ttaggcgtgg caattctata atgatacgca ttatctcaag agcaaattct gtcacttctt
     8701 ctaatgaagt gaaccgctta gtaacaggac agattccgca tgactgataa caataccgcc
     8761 ctaaagaaag ctggcctgaa agtaacgctt ccacgtttaa aaatcctgga agttcttcag
     8821 gagccggaca accatcacgt cagtgcggaa gatttataca aacgtctgat cgatatgggt
     8881 gaagaaattg gtctggctac ggtatatcgc gtactgaacc agtttgacga cgctggtatc
     8941 gtcacccgcc acaattttga aggcggtaaa tccgtatttg aactgacaca gcaacatcac
     9001 cacgatcacc tgatttgcct cgactgcggc aaggttatcg aatttagtga tgattccatc
     9061 gaagcgcgtc agcgtgaaat tgccgcaaaa catggcattc gcctgactaa ccacagtctc
     9121 tatctttacg gtcactgtgc cgaaggcgat tgccgcgaag atgagcacgc gcacgaaggc
     9181 aaataagcca gcctgaacga gaaaagccaa cctgcgggtt ggctttttta tacaagggaa
     9241 ataagaaaag cgagagttac agctctcact tatttgttat tactgcgtat ttcctgccag
     9301 accttatcac aatcggcttt caccgcctga tcatttccgg tttgcgttgc acgcagacac
     9361 tgctgataat ccagtacgcg tacactttcc tggtcggcaa actgctgatg ttgtttctct
     9421 ttcttcagca cgtttaacac gctctggcag gcttcaattt tttctggcga accttgtgcg
     9481 gtgttgatac aggcgctgta tgcctctttc agacgactgt cttcctgtgg cgcggttgat
     9541 tgcgcacaag ctaccagccc cgatgccatt atggcgatga gaatcagttt tttcatagca
     9601 aattcctcaa acgtgcggca ggcacaaaac ctgccgcgtc gggcatcaga agatggtgaa
     9661 tggtgcgatt accataaatt tcacgtcacg ctcatcctgg aagatgttac cgtaaccgcc
     9721 gccccagctt gggatgtcag agtggttgtc gtattcggtg aagtgcagtt tgaacatcgt
     9781 gcctttggcg cgaccgtcct gaatggtgta gaccgcatcc aggctgtagg cagactcttc
     9841 aatagtccgg tttttgtcgt agtacgcatc cggattgctc tgccaggtcg caggtttagc
     9901 atcccatgcg taaacgtagg aagcgccgat ggcgaagcct ggaagattcc agtttttcag
     9961 gtcatacatc gcaccgaaga agaccgcttt ttcgccgttg gcgttgaagt cggaacggtt
    10021 atcccaccag atatccaggc ggccgttgga ggaagcgtag gttggagtca tacgttgcag
    10081 gaagtatccc tgctgaccgt cagccttaac ccaggtgcct tcgaggcgca aatccactac
    10141 gtcagccgcc cggtaaccaa aggtcaacgc ttgcagccag gcggtgccgt cataaaggtc
    10201 gttgacgctg cgatcgtcaa ctttatcgcg cgtaccatag aactggtagc tggtggttaa
    10261 cgggctaccg gcgatatcaa atttgtagct ggctttggca aaatattgat cgatataccc
    10321 ttccgcctga ccaaatgccg cttccagtac gaagttattt ttgaagtcgt atttcgcccc
    10381 aagggagtgc agataatcaa ctttggtggt tttatcgttc tgataaaact catccatttc
    10441 cagatgccac ggcgctttgt attcgttggt ccacatgtag gagaaactca aggcaccagc
    10501 atcgccgtaa tcaaaattcg ccccggcttc cgccccctga taagtacctg gcataaagct
    10561 ccagtgcggc gctaacagcg tttgaccagt tggctgaatg taacctgccc tcgcccaaac
    10621 cggaccgtat ttaaatttgg ccgcagcttt atacaggctt ataccgcttt tgtcgccgga
    10681 ccagtcttcg tcataggctt tattactttt tgaaaacgcg atttcgttcg ggtgggagct
    10741 gtcgccgttt tccgccattt caatcgccgt aaacgcagca atatcaaggc cgaacatatc
    10801 agcagcataa ccggactgaa aatcgaggtt ggcattccag gtggagtgag aaaggttggt
    10861 tttgtatttg tcgccgtcgg taacatcttt acggtcgcgt tcacgctgcc agtaatatat
    10921 accgccggtt aaggttgaat cgtcgatgaa tccttcggcg cgagcctccg gcattgccat
    10981 aaagcccgac attgctgtaa caccggcgat agccagcgcc agcgtactac gtttgccact
    11041 aaacgtacgc atgggttaat cctctttgac gtataaattg ctgcaccaaa ggtgaacagc
    11101 gaaaaaataa aaattaaaaa agtagcgttg agaaaagtct cgctgactac aggaacagac
    11161 tatttttcgc gacgcgaatt atcaatctct ttctgtaacc agaatgtaag aacatgagca
    11221 accgcacata ttttgatgat ttttgttaca aggcttaagt ttgcggttga aaggcttgca
    11281 gaagaataaa agcgtgaatt tccagcaaga tgaaagcggt taca
//
LOCUS       DAZGSP010000142        11252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZGSP010000142 DAZGSP010000000
VERSION     DAZGSP010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.143"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            152..628
                     /locus_tag="ACHK74_004528"
     CDS             152..628
                     /locus_tag="ACHK74_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFP0223231.1"
                     /translation="MDGELKNMKLNINQLAALSGLHRQTVAARMADVPLAPGSNEKKK
                     LYLLTDLITSLLEKPPSSEDEDMDPHARKAWYQSERERLKFQHETVQLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
     gene            625..2748
                     /locus_tag="ACHK74_004529"
     CDS             625..2748
                     /locus_tag="ACHK74_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP0223232.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPGRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQTARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEESLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAG
                     VTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQERSGETVSPELTAGAEK
                     RRRRKKKLRTERAEDNPWITSGGWL"
     gene            2745..2957
                     /locus_tag="ACHK74_004530"
     CDS             2745..2957
                     /locus_tag="ACHK74_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223233.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            2957..4459
                     /locus_tag="ACHK74_004531"
     CDS             2957..4459
                     /locus_tag="ACHK74_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0223234.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVAMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            4449..6428
                     /locus_tag="ACHK74_004532"
     CDS             4449..6428
                     /locus_tag="ACHK74_004532"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFP0223235.1"
                     /translation="MKNKSWFRMQAGGPGDADIYIYDEIGFWGVTAKQFVSELNALGD
                     ITHINLHINSPGGDVFEGIAIFNSLKNQGATITVYVDGVAASMASVIAMAGDTVIMPE
                     NAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTGKTTDEIAAMLADETW
                     MSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNMITPPRNSAPRDTTVT
                     IPAPAVTEPSPVPAVSDEATIRACVMAEQKARMSGINDLFAMFGGRYQTLQAQCVADP
                     DCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIRQAMLARAGFENVEKD
                     NAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDFGNILLDVSNKGLIHG
                     WEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEYKYITTSDRKETIALA
                     TYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKVLTDNPKLSDGKALFH
                     ADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVPVALETVANQTIKSAS
                     VKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTDTIEVAWLDGVDTPYI
                     DQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            6515..6841
                     /locus_tag="ACHK74_004533"
     CDS             6515..6841
                     /locus_tag="ACHK74_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFP0223236.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            6834..7115
                     /locus_tag="ACHK74_004534"
     CDS             6834..7115
                     /locus_tag="ACHK74_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFP0223237.1"
                     /translation="MSDLFTRMCCRMDVATVRMMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            7118..7741
                     /locus_tag="ACHK74_004535"
     CDS             7118..7741
                     /locus_tag="ACHK74_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0223238.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKIPTAGPLAETFERERDRMYREKLPAQMMTAMAHQLRLVLKRK"
     gene            7754..8152
                     /gene="gpU"
                     /locus_tag="ACHK74_004536"
     CDS             7754..8152
                     /gene="gpU"
                     /locus_tag="ACHK74_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFP0223239.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKITYEM"
     gene            8160..8909
                     /locus_tag="ACHK74_004537"
     CDS             8160..8909
                     /locus_tag="ACHK74_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0223240.1"
                     /translation="MATPNPLEPVKGSGTTLWVYTGTGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SETRAYKILFPNGTVDVFRGWVSAIGKAVTAKEVITRTVKITNIGRPSLAEDQGDITP
                     VTGITVTPPTGNVAKGQNITLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKG
                     VAAGKAQIPVVTGNGEFAAVAEITVTDVAAG"
     gene            8929..9360
                     /locus_tag="ACHK74_004538"
     CDS             8929..9360
                     /locus_tag="ACHK74_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP0223241.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEEAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTASPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNALTDDDFSAGKSSTAS"
     gene            9387..9791
                     /locus_tag="ACHK74_004539"
     CDS             9387..9791
                     /locus_tag="ACHK74_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP0223242.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFHDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRREEAQTERPDEDDMLMRKAAGLSGGVRFGADGKEI
                     VTVSDDMRSSTEDEAMLMMVSEGIAGGVRYGG"
     gene            9781..>11252
                     /locus_tag="ACHK74_004540"
     CDS             9781..>11252
                     /locus_tag="ACHK74_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP0223243.1"
                     /translation="MAGNFADLTAVLTLDSTRFSEEAARVKKELGETSDLADLMAGRV
                     SQSFKKQAAAVEQGLSRQALAAQKAGMSVGQYKAAMRMLPMQFTDVATQLAGGQNPWL
                     ILLQQGGQVKDSFGGMIPMFRGLAGAISLPMVGVTSLAVATGALAYAWYQGDSTLSAF
                     NKTLVLSGNQSGLTAERMLTLSRAGQAAGLTFNQAGESLAALVSAGVRGGEQFDAINQ
                     SVARFASASGVEVDKVAEAFGKLTTDPTSGLIAMARQFRNVTAEQIAYVAQLQRSGDE
                     AGALQAANEAATKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQE
                     MLIKAEAAFKKADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQD
                     KNAQQQSDTEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTL
                     MAAAKKDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQA"
BASE COUNT         2608 a         2689 c         3551 g         2404 t
ORIGIN      
        1 catcatgatg gtaacttgtt gtttttaatg ctgttagtat taaaaagatg atgattgtgg
       61 ttgttttttg ttcgacatct ttatatggcg gcatttcttt acaaaaaaaa cagagtcact
      121 tctgttctgc ggtttatgtg gagggatgta aatggacggc gagctgaaaa atatgaagtt
      181 aaatattaat caactggcag ccctttcagg tctgcaccgg cagactgttg ccgccagaat
      241 ggcggatgtt cctcttgcac caggcagtaa tgaaaagaaa aaactgtatc tcctgacgga
      301 tttgataact tcgttgctgg aaaaaccacc atcttccgaa gatgaggata tggatcctca
      361 cgctcgtaag gcatggtatc agtccgagcg cgagcgtctt aaatttcagc atgaaactgt
      421 tcagcttgtg ccagtcagtg atgtcaggcg gtccttttct gtcgtggtga aagcgatagt
      481 tcaggtactg gaaacctggc ctgaccggct ggagagggac agggggtgga ctgcatcaca
      541 actgaatgaa gtacagattg tggttgatga gatccgcgac acactggaaa aggcagtcat
      601 tgactgttgt gatgaggccg atatgtgaat caggtgaacg agagccatag ccgcgcatcc
      661 gatatctggc gcgaagtggc ctcgctgttt cgcccacctg gccggttacc agtagcggaa
      721 gccatcaggc gttatatgcg ggtaccacgg ggagccaata cttccggtcc gtgggagtca
      781 tcgctgacgc cctatatgat agaccccatt aatacattat cagcccgtga atatgacgcg
      841 gtggtgtttg tcggaccggc gcgaaccggg aaaaccgaag ggctgattga tggctggatt
      901 gtgtacggca tcatctgtga tccggcggat atgctggtgg tgcagatgac cgagacgaag
      961 gcgcgtgagc attccagaac gcgtctttcc aggacgtttc gccacagtcc ggaggtcagc
     1021 aagcgcctca gtccttcccg taatgacaac aacgtccacg ataaaatgtt tcttgacggc
     1081 tcattcctga aaattggctg gccgtcgatc accgtatttt cctcctcgga ttaccgtcgt
     1141 gtggcgctga cggattatga ccgttttcct gagaatgtcg acggtgaagg ggatgccttc
     1201 acgctggcct caaagcgtac caccaccttt atgtcctcgg ggatgaccct ggtcgagagt
     1261 tcaccggggc gggatatcac tgacaccaaa tggcgttgtg gtggagcaca tgaggcaccg
     1321 ccaacaacgg ggatcctgtc actgtataac cggggagacc gccgccggtg gtactggccg
     1381 tgtccgcact gcggggaata ttttcagccg gtgatggata acatgaccgg ttaccggaat
     1441 aaccctgatt ttgtggctgc cgggcagacg gcccgcctga tgtgtccgca ctgccgcggc
     1501 ctgattgctc cggaacagaa gcgtgaactg aataaccagg ggatctggct gcgggaaggc
     1561 gaacgggcag cggcggatgg cagtatcacc ggaacccccc gaaattccag aatagcaagt
     1621 ttctggatgg aggggccggc tgcggcgttt cagacctggg aacaactgat ttttaagctg
     1681 ctggcggcag aagaagagta tgagcgaacc ggcagtgaag agagcctgaa agcggtggtg
     1741 aacaccgata tcggacgacc ctatctgccc cgttcagcca cggaacagcg taaaagtgaa
     1801 ctgcttgaac agcgtgccga gccgtttccc aggcgatctg tgccggatgg tgtgcgtttt
     1861 attgaggcaa cggttgacgt acagggcggt aaaaatcgcc gttttgttgt gcagatcacc
     1921 ggatacggag agcaggggga acgctggatt gttgatcgct acaacatccg gcattcactg
     1981 cgctgcagtc ccaacggtga aagtctgccg gttgatccgg cggcatatcc ggaggactgg
     2041 gatttgttgc tgacggatgt gttccataaa acatggccgc tggcttctga tccggatgtg
     2101 cgcatgcgtc tgatggccat ggcggtggat acgggagggg aagccggggt gacagataac
     2161 gcctatcgtt tctggcgtcg ttgccggagt gacggactgg gcaacagggt gtttctgttc
     2221 aagggggatg gtcttcgccg tgacaggctg attaaccgta ccttcccgga taataccggc
     2281 agaagtgccc gccgtgccag agccagtggc gatgtcgcgc tgtggctggt tcagacggat
     2341 gcgtttaagg accgtgtaaa taatgccctg tggcgtgaca caccagggcc gaattatatc
     2401 cactttcccg actggctggg gcggtggttt tacgatgagc tgacctatga agagcgcggc
     2461 agtgacggaa aatggcgaaa accgggcagg ggtgctaacg aggcgtttga cctgctggtt
     2521 tatgcggatg cgcttgctgt tctgcatggt tatgaaaaga tccgctggcc ctccgcaccg
     2581 gactgggcac agcgggaaac gtggctcgtc ttcccgcagg agcgttctgg tgaaacggta
     2641 tccccggaac tgacggccgg ggcagaaaaa cgccgtcgcc ggaagaaaaa actgcggacg
     2701 gagcgtgcgg aagataatcc atggataaca tcaggaggct ggttgtgagc acagaagaag
     2761 ccagagaaat gattcagcgg taccgtgaag cggaaatggc cgtactggag gggaagtctg
     2821 tcaccttcaa cgggcagcaa ctgacgctgg aaagcctttc tcagatccgc gccggacgtc
     2881 aggagtggga acgcaggctt gccgcgatgg tgagccgcag gcggggaaaa ccgggattta
     2941 aactggcgag gttttaatgg caattattga tgatgtgata ggcgtgtttt cccccggatg
     3001 gaaagcagcc agactgcgtt caagggcgtt aatcatggcc tatgaggcgg tgaaaccgac
     3061 ccggacacat aaagcccggc gggaaaatcg ctctgctgat cagctcagta aatacggtgc
     3121 ggtttccctg cgggagcagg cccgttttct ggatatcaat catgacctgg tgattggtgt
     3181 gtttgacaag ctggaagagc gggtgattgg tgccagggga attattgtgg agcctcagcc
     3241 attacgaaaa aacggggaaa tggcggcaga gctggctgcg gatatccgcc ggttgtgggc
     3301 tgaatggtcc gtgagtccgg atgtgacagg gcagtatacc cgtcccgtgc ttgaacgttt
     3361 actgctgcgg acctggctgc gggatggtga agtgtttgcg cagatggtca gtggtgcggg
     3421 aaacggtctg gaacggacgg cgggagtgcc attctggctt gaggcgatgg agccggattt
     3481 tgttcccatg cgcactgatg aatccgccgg actgaatcag ggggtttttc ttgatgagtg
     3541 gggaagaccg aaaaaatatc tggtttataa aaattatccg gtcagcggcc ggcagagtga
     3601 tacgaaagaa atcgctgccg gaaaaatgat ccacctgaag ttcactcgtc gtctgcatca
     3661 gacgcgaggc tcatccatgt tatcgggggt gctgatgcgg atcagtgccc ttaaagagta
     3721 tgaggatgcg gaactgacag cggcgcgtat tgctgcggcg ctgggactgt atatccgtaa
     3781 aggtgacgga caggactatg aagatccggg gatcaaagaa accgagcggg aagtccatat
     3841 caccccgggt attatttatg acgatttgcg caagggcgag gatatcggca tggtcaaatc
     3901 tgaccgtccc aatcccaacc ttgaaacttt ccgcaacggc cagttgcgtg cagtggcagc
     3961 tggcagtcgt ctgagttttt ccagtgcggc gcgtaactat aacggcacct acagcgccca
     4021 gcggcaggag ctggtcgagt ccacggatgg ttacctgatc ctgcaggact gttttattgg
     4081 tgcggtaacc cgcccggtgt accggacatg gctgaatatg gtggttgcgg caggtctgct
     4141 gaaaattccg gcggatgtgg cgatgaaaac gctatataac gcgacgtatt ccggtccggt
     4201 gatgccgtgg atcgacccgg ttaaggaagc tgaagcctgg agaattcaga tccggggtgg
     4261 tgcagcgaca gaatctgact gggtgcgtgc cggcgggcgc aatccggatg aggtcaaacg
     4321 tcgccgcaag gctgaaattg atgaaaacag cagactgggg ctggtctttg atactgaccc
     4381 cgtcaacgac aaaggaggca acagtgccgg aactgaacga cagtatcagc gtgacaccga
     4441 aagccagcat gaagaataaa tcctggttca ggatgcaggc tggggggccg ggtgacgcgg
     4501 atatttatat ttatgacgag attggtttct ggggagttac cgcgaagcag tttgtcagcg
     4561 aactgaatgc tctgggtgat atcacccaca ttaatctcca catcaactca ccgggtggcg
     4621 atgtctttga aggcatcgcc atttttaatt ccctgaaaaa tcagggggcg accattaccg
     4681 tgtatgtgga tggcgttgcc gcctcgatgg catctgtgat tgcgatggcc ggtgatacgg
     4741 tcattatgcc ggaaaatgcc ttcatgatga tccataagcc atggggattc agtggcgggg
     4801 atgctgagga tatgcgcagt tatgctgatt tgctggataa agtcgaatcg gtactgttgc
     4861 cagcctatgc gcagaaaacc ggaaaaacca ccgatgaaat tgccgccatg ctggcggatg
     4921 aaacctggat gtccggtgcc gaatgtctgg cacacggatt tgctgaccag gtgacacccg
     4981 ctgttgaggc aatggcatgt attcagtcaa aacgtacaga ggaatttaaa aagatgccgg
     5041 aatccatccg aaacatgatt actccgccac gcaacagtgc cccgcgtgat accacagtga
     5101 caatccctgc accggcggta acagaaccat caccggtacc agcagtgtct gatgaggcga
     5161 ccattcgcgc ctgcgttatg gctgagcaga aagcacgcat gtcaggcatt aacgatctgt
     5221 ttgccatgtt cggcggtcgc tatcagacgc ttcaggcaca gtgcgtggct gatcctgact
     5281 gttcgctgga aatggcccgt gaacgtctgc tgaatgaaat gggcaaggag tcctcgccga
     5341 ccaacaaaaa cacaccggcc catatttatg ccggaaacgg caattttgtg ggggacggga
     5401 tccgccaggc gatgctggcc cgtgccggat ttgaaaatgt cgagaaggat aacgcctata
     5461 acgggatgac cctgcgtgaa tgggctcgca tgtcactgac ggagcgcggt attggggtgg
     5521 ccagttataa ccccatgcag atggtcgggc tggcgctgac gcacagcacc tctgattttg
     5581 gcaatattct gctggatgtg tcgaacaagg ggctgatcca tggctgggag gaatcagaag
     5641 aaaccttcca gaagtggacc cgtaagggac gcctgtcaga cttcaaaaca gcgtatcgcg
     5701 tggggatggg cggttttggt tctctgcgcc aggttcgtga gggggcggag tataaataca
     5761 tcaccacctc agatcgcaag gagaccattg cactggccac ttacggggag attttctcca
     5821 tcacccgcca ggccattatc aatgatgatc tgaatatgct ggtggacgtg ccgatgaaga
     5881 tggggcgtgc ggcgaaggca acgattggtg acctggtcta caaggtgctg acggataacc
     5941 cgaaactgtc tgacggtaag gcgctgttcc atgccgatca caaaaatatt gccaccgggg
     6001 ggatctccgt ttccggactg gatgcggccc gtcagatgat gcgcctgcag aaagaaggcg
     6061 atcgcgccct gaatatccgt ccggccttta tgctggtacc ggtggcactg gagacggtgg
     6121 cgaaccagac catcaaatcg gccagtgtga aaggggcgga tgcaaacgcc ggtgtcatta
     6181 accccatcca gaactttgct gaggtgattg cagaagcgcg tcttgatgcg gcagatccga
     6241 aaacctggta tctggcggcg gcacagggca ctgacaccat tgaagtggcc tggctggatg
     6301 gtgtggacac gccatacatt gatcagcagg aaggtttcac cactgacggt attgccacaa
     6361 aaatccgtat tgatgccgga gtggcaccac ttgactggcg cgggctggtg cgttcgtcgg
     6421 tggcctgata accgcgttat cacaatcact gcccgaaagg gcttttttat gcctgaaaaa
     6481 cagccccaca ggggctgtcc ggagaaacag cattatggcg aaaaattttg tacaggacgg
     6541 taccaccatt gaactggtga atgccggaga tcagaccatc ctgagcggtg ctgcggtggt
     6601 ggtcggcagt atggtggccg tggccattac cgatattcct gccggtgagg ccggtgacgg
     6661 ttttgccgaa ggcgtgttcc tgctgcccaa acagtctgct gacgacattc agtccggcgc
     6721 ggtggtttat ctgaaggacg gggttgtgca gctggctgca gacggtgcgg tggcagcggg
     6781 agtagcctgg gaaaatgctc ctgcaaacag cgccactgtg gcggtaaaaa tcaatgtctg
     6841 atctgtttac gcgaatgtgt tgccggatgg acgtggcgac cgttcggatg atgggcaaac
     6901 aggcggagat taacggcgtc gtgtacgacg tgatgccgga ggaagagtcc gcggagatgg
     6961 gggcgctttc gggcagccag ttgtcactgg tggtgttttc agcccggtac cgtccggccc
     7021 gtcatgatgt tgttgtgttt gcggggcgca cactgacggt gacccgttat gacacgtaca
     7081 acggtaaacc ccggattttt gtcgaacagg aatgagtatg gcaataaaag gtctggcgca
     7141 ggccatgaaa aatctggatg caattgatcg ccgtgccgtt ccccgggcct ctgccacgac
     7201 actgaaccgc gtggcggggg ccattattgc gaaaacggcc tcttcagttg ccagggagct
     7261 ggccgttccc cgtcgtctta tccgtgcccg catccggtta agtccggcac gaccggataa
     7321 ggtttacgca aaggtttaca tcaataccgg caacctgccc gccatcaaac tgggggaggc
     7381 ccgcgttcga ctttcccgca gaaaacggag aaagaaagga cagcgtgcgg ccctgaaagg
     7441 gggcggcagt gtgctgattg tggggaaaag acggatcccg gacgccttta tcacccggct
     7501 ggctaacgga cgctggcatg tgatgcagcg tatgccgtgg gcatcatcgt ccaccggcgc
     7561 ggacagcaaa gggaggccga aacgccaccg tctgccgatc gaagtggtga agattccgac
     7621 tgccggaccg ctggcagaaa cctttgaacg tgaacgggac cggatgtacc gggaaaaatt
     7681 accggcgcag atgatgacgg ccatggcgca tcagttacgc ctggtgctga aacgaaaatg
     7741 acagggaggg tgtatgaaac accgtgaaat acgggcggca gttctgtctg ccctgaaaga
     7801 aaatatttct gagagggtga gctggtttga cggtcgtccg gtttttattg atgaacagga
     7861 actgcctgct gttgctgttt acctgacaga tgcgtctgct gctgacgagt tcgttgatga
     7921 gggaacctgg gaggcgacac tgcatattga ggtttttctc agggcaaaag aaccggactc
     7981 ggcactggat atgtggatgg aagaaaaaat tcttcctgcg ctggaggcag ttcccgggct
     8041 cagtgcatta ctgctgaaga tgaatcttca ggggtatgac taccgccggg atgatgagtt
     8101 tatgatgtgg ggatcggcag atctcctgtg gaaaattacc tacgagatgt gaggacgata
     8161 tggcaacacc aaatcctctt gagccggtga aaggttccgg taccacactg tgggtgtaca
     8221 ccggcactgg tgatgcttat gcaaacccgt tgtcagacga tgactggcag cgactggcga
     8281 aggtgaagga tctgaccccc ggcgagatga cggcggaatc ctacgatgat aactatctgg
     8341 atgatgagga tgctgactgg gtatccaccg ggcaggggca gaaatctgcc ggtgacacca
     8401 gttttacgct ggcctggaag ccgggtgaga aagggcagcg cgatttgatt gcctggtttg
     8461 acagcagtga gacccgggcc tacaaaattc tcttcccgaa cggtacggtg gatgtgttcc
     8521 gtggctgggt gagcgccatt ggtaaagcgg tgaccgccaa agaggtgatc acccgtacgg
     8581 taaaaatcac caatatcggt cgtccgtcgc tggcggaaga tcagggggac atcacaccgg
     8641 tcaccggtat taccgtgacg ccaccaacgg gcaatgtggc aaaaggtcag aatatcaccc
     8701 tgaccgtggc cgttcagccg gaaggcgcga cggataaaac gttccgcgca acctcagcga
     8761 atcagaattt cgccaccatt accgtgaagg ggaacacgat cacggtgaaa ggtgttgcgg
     8821 caggtaaagc acagatccct gtggtcaccg gcaatggtga gtttgcggcg gtggcggaaa
     8881 ttaccgtcac ggatgttgca gcgggttaag ctgagggagt gataaagcat gtttctgaaa
     8941 acagaacaat ttgaatataa cggtgtgtct gtcacgcttt ctgagctgtc tgcgctgcag
     9001 cgtattgagc atcttgccct cctgaaacgg cgggcagaag aggctgaagc cagcggtaac
     9061 ctgcaggtga gcgtggagga ccttgtcaga accggggcgt ttctggtggc gatgtccctg
     9121 tggcataacc atccacagaa aacggcgtca ccgtcaatga atgaggccgt gatgaagata
     9181 gagcaggaag tgctcaccac ctggcctgcc gatgccattg cccgggcgga agacgttgtg
     9241 ttgtgcctgt ccgggatgat cgaagctgtt cgtccggata ctgatattac tgaagtggcg
     9301 aaaaataacg cgctgactga tgatgatttt tctgcgggaa agtcttcgac ggcgagctga
     9361 actttgccct cagactggcg cgtgagatgg ggagacccga ctggcgcgcc atgcttgccg
     9421 ggatgacatc caccgaatat gccgactggc accgttttta ccgcacgcat tattttcacg
     9481 atactcagct ggatatgcat ttttccgggc tgacgtacgc cgtactcagc ctgttttttt
     9541 gcgatccgga tatgcatccc tcggatttca gtctgcttgt cccccggcgt gaggaagcgc
     9601 agacggagag gccggatgag gacgatatgc tgatgcggaa agcggcagga ctttctggtg
     9661 gtgtccgctt tggggctgac gggaaggaaa tcgttacggt cagtgatgac atgcggagca
     9721 gcacagagga tgaagccatg ctgatgatgg tgtctgaggg aattgcagga ggtgtacgct
     9781 atggcgggta attttgccga tctgacagct gttcttacac tggattcaac ccgtttttct
     9841 gaagaggctg cacgggtaaa gaaagaactg ggtgaaacca gtgaccttgc ggatttgatg
     9901 gccgggcgtg tcagccagtc ttttaagaaa caggccgctg ccgttgaaca ggggctgagc
     9961 cgtcaggccc tggctgcaca aaaagccggg atgtccgtcg ggcagtataa agcggccatg
    10021 cgtatgctac ccatgcagtt caccgacgtg gccacgcagc ttgccggtgg tcagaatccc
    10081 tggctcatcc tgctgcaaca gggcggtcag gtgaaggact cattcggcgg gatgatcccc
    10141 atgttccggg ggcttgccgg tgcgatcagc ctgccgatgg tcggggtcac ctcgctggcg
    10201 gtggcgaccg gtgcgctggc gtacgcctgg taccaggggg attccacgct ttcagcgttt
    10261 aataaaaccc tggttctttc cggtaatcag tcaggactga cggcagagcg catgctgacg
    10321 ctctccagag ccgggcaggc ggcagggctg acgtttaacc aggcgggaga gtcactggca
    10381 gccctggtca gtgccggtgt gcgtggtggt gaacagtttg atgccattaa ccagagtgtg
    10441 gcgcgttttg cgtctgcctc cggtgtggag gtggacaagg ttgcagaggc tttcggaaaa
    10501 ctgaccaccg acccgacgtc ggggctgatt gcgatggcac gccagttccg taacgtgacg
    10561 gcagagcaga ttgcgtatgt tgctcagttg cagcgttccg gcgatgaagc cggggcattg
    10621 caggcggcga acgaggccgc cacgaaaggg tttgatgacc agacccgccg cctgaaagag
    10681 aacatgggca cgctggagac ctgggcagac aggactgcgc gggcattcaa atccatgtgg
    10741 gatgcggtgc tggatattgg tcgtcctgat accgcgcagg agatgctgat taaggcagag
    10801 gctgcgttta agaaagcaga cgacatctgg aatctgcgca aggatgatta ttttgttaac
    10861 gatgaagcgc gggcgcgtta ctgggatgat cgtgaaaagg cccgtcttgc gcttgaagcc
    10921 gcccgaaaga aggctgagca gcagactcaa caggacaaaa atgcgcagca gcagagcgat
    10981 accgaagcgt cacggctgaa atataccgaa gaggcgcaga aggcttacga acggctgcag
    11041 acgccgctgg agaaatatac cgcccgtcag gaagaactga acaaggcact gaaagacggg
    11101 aaaatcctgc aggcggatta caacacgctg atggcggcgg cgaaaaagga ttatgaagcg
    11161 acgctgaaaa agccgaaaca gtccggcgtg aaggtgtctg cgggcgatcg tcaggaagac
    11221 agtgctcatg ctgccctgct gacgcttcag gc
//
LOCUS       DAZGSP010000143        11080 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZGSP010000143 DAZGSP010000000
VERSION     DAZGSP010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.144"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..545)
                     /gene="fcl"
                     /locus_tag="ACHK74_004541"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(<1..545)
                     /gene="fcl"
                     /locus_tag="ACHK74_004541"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFP0223244.1"
                     /translation="MSKQRIFIAGHRGMVGSAITRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIP"
     gene            complement(548..1669)
                     /gene="gmd"
                     /locus_tag="ACHK74_004542"
     CDS             complement(548..1669)
                     /gene="gmd"
                     /locus_tag="ACHK74_004542"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFP0223245.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLKEMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(1696..2244)
                     /gene="wcaF"
                     /locus_tag="ACHK74_004543"
     CDS             complement(1696..2244)
                     /gene="wcaF"
                     /locus_tag="ACHK74_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFP0223246.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATLFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(2260..3006)
                     /gene="wcaE"
                     /locus_tag="ACHK74_004544"
     CDS             complement(2260..3006)
                     /gene="wcaE"
                     /locus_tag="ACHK74_004544"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFP0223247.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNSIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            complement(3017..4234)
                     /gene="wcaD"
                     /locus_tag="ACHK74_004545"
     CDS             complement(3017..4234)
                     /gene="wcaD"
                     /locus_tag="ACHK74_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFP0223248.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGVGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(4209..5426)
                     /gene="wcaC"
                     /locus_tag="ACHK74_004546"
     CDS             complement(4209..5426)
                     /gene="wcaC"
                     /locus_tag="ACHK74_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFP0223249.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAGFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(5423..5911)
                     /gene="wcaB"
                     /locus_tag="ACHK74_004547"
     CDS             complement(5423..5911)
                     /gene="wcaB"
                     /locus_tag="ACHK74_004547"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFP0223250.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     AENMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(5914..6753)
                     /gene="wcaA"
                     /locus_tag="ACHK74_004548"
     CDS             complement(5914..6753)
                     /gene="wcaA"
                     /locus_tag="ACHK74_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFP0223251.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(6931..9093)
                     /gene="wzc"
                     /locus_tag="ACHK74_004549"
     CDS             complement(6931..9093)
                     /gene="wzc"
                     /locus_tag="ACHK74_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFP0223252.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(9096..9539)
                     /gene="wzb"
                     /locus_tag="ACHK74_004550"
     CDS             complement(9096..9539)
                     /gene="wzb"
                     /locus_tag="ACHK74_004550"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFP0223253.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(9545..10684)
                     /gene="wza"
                     /locus_tag="ACHK74_004551"
     CDS             complement(9545..10684)
                     /gene="wza"
                     /locus_tag="ACHK74_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFP0223254.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
BASE COUNT         2635 a         2897 c         2743 g         2805 t
ORIGIN      
        1 gggatcacat gcgagttact cggatggaag ttgtcgtgtg gcccgtacag gttggttggc
       61 atgaccgagc ggtaatcgcg tccgtactgg cggttgtaag attcgcacaa tttgatcccg
      121 gcgattttgg caatggcata aggctcgttg gtcggctcca gcgtaccctg caacagctcg
      181 ctttctgcca tcggctgttt tgccagtttc gggtagatac aggacgatcc gagaaacagc
      241 agtttgttca cgtcgttctg atgcgcggcg tgaatgatgt tgctctcaat catcatgttc
      301 tggtagatga aatccgccgg ataggtgttg ttggcaacaa tgccgcccac tttcgccgcc
      361 gccagataga cctggtcaat gcgttcgctg gcaaagaaat catgcaccgc gcggctgtcc
      421 agcaggttca gctcgtcgcg ggtgcgtaat accagttcca catcaccgcg ctgttcgagc
      481 tgccgcgtga tggcggaacc gaccatccca cgatggccag cgataaaaat gcgttgttta
      541 ctcatgctta tgactccagc gcgatcgcca cgtcgtagcc gtgagatttc agcagagagt
      601 gttttttcgc cgcttcgagg tcattagcca ccatttcaga caccatctct ttgagggtga
      661 tttccggttt ccagcccagt ttttcgtgcg ctttggtcgg gtcgccgagc agcgtttcaa
      721 cttccgccgg acggaagtaa cgcgggtcaa cggcgataat cacatcaccc ggtttaacgc
      781 ccggcgcgtc atgcccggtg acggaaacca caatgccctt ctcttcaaca cccgtgcctt
      841 caaagcgcag tttgatgccc agctgtgccg ctgccatttc cacgaactga cgcacggagt
      901 actgaacgcc ggtcgcgata acgaaatctt ccggctgttc ctgttgcagc atcatccact
      961 gcatttttac gtagtctttg gcgtggcccc agtcacgcag ggaatccata ttgccgaggt
     1021 acaggcacga ctccagcccc tgggcgatgt tggcgattgc gcgggttatt ttgcgggtaa
     1081 cgaaggtttc gccacggcgc ggggattcat ggttgaagag aataccgttg caggcgtaca
     1141 tgccgtagga ttcgcggtag ttaacggtga tccagtaggc gtacagtttg gcgaccgcat
     1201 acggagatcg cgggtagaac ggcgtggttt ctttctgcgg aatttcctgc accagaccat
     1261 acagttcaga ggtggaagcc tgatagaaac gggttttctt ttccagaccg aggaagcgga
     1321 tcgcctcgag caggcgcagc gtacccatcg cgtcgacgtc agcggtatat tccggtgact
     1381 caaaagaaac cgcaacgtgg ctcattgccc ccaggttgta cacttcatcc ggctgtactt
     1441 cacgcaaaat gcgcgtcagg ttggaggtat cactcaggtc gccataatgc agatggaatt
     1501 ttgggttgca ggtgtgcgga tcctgataaa tgtgatccac gcgctcggtg ttgaatgacg
     1561 atgcacgacg cttaatacca tgcacctcgt aacctttttc cagcagaaac tctgccaggt
     1621 aagaaccgtc ttgtccggtt acaccggtga tgagagcgac ttttgacatg tcttattcct
     1681 ctgtattttt tgaatttatt cagtttcaac gcgttcgcgt atcaccactg cgggattccc
     1741 ccggcaaacc acatttgccg gaagcgattt aaaaacactg cttcgcgccc ccacgacggt
     1801 gccgtcgccg attgtgacgc ctggggcaac aaagacatcg gttgccagcc agcatttctc
     1861 gccaatcaca ataggcgtgg cgttaatggt gaaatgttga cttgcatggt cgtggctacc
     1921 ggtgcataaa taactttttt gcgatatcac cgaatgtgcg ccaatggtta tttcaccgag
     1981 ggtatataaa ttgacctcat cgccgaccca cgcgtaatca cctaaggtta atttccacgg
     2041 ataggtaatt tttactgacg gacgaataac tacgttttta cctatttttg ccccgaataa
     2101 acgtaataaa aatgcccgcc agcgatacaa tacttgtggc gaccaggcaa ataatgttgc
     2161 ctgtactgcc caccacaatt gcactttaat agcgttgccg ccccggaacc ctttcggcac
     2221 cgagaaaccg cttaaatctt gcatcgtttt cccttatatt cagcttttgt tatataaggc
     2281 tttcgtcttt gaggtagtac gttggcgtaa atgccaggat aattctgccc agaagccagg
     2341 cacatgtaat atttgccgtt ggactttttt ggcgtcagca cacaattcca tattattggt
     2401 ggtagatacc ccacccatag aaaattcaga caccaggcca ttgagttttt taaatgcata
     2461 acctgcttta tacattttgg ctgccagcgc gtaatcagaa gaaactttat attccaggtc
     2521 ataacgccat tttttcaagc cggataccgg gaaaaatatc gcctgatggc tggcgggcag
     2581 gctgtgataa atataccaac ccggtttggc gctacgttta attttatgcc cgtcgccaaa
     2641 atccagcagc gcatcgccgg tgatcatcat gttatctttt tgcattttta acttacggac
     2701 aaaatttgcg gcatcctgat gaaaaatatc gcccgaattg agaaacaacg cgaacttgcc
     2761 ttgtgccatc gcaatgccct tgttcatggc gtcgtagata ccgttatctg gctcgctgac
     2821 aaagcgtagg ttatagatac tattgagatt ttccagatac tcacgggtac cgtcgttgga
     2881 accgccatcg acaacaatcc attcgaagct gatatcgtcc gcctgcgcca gatgcgccag
     2941 cgaggcatgt gttttgacta tcccttcgag gttacgaaac gcgacagtga ttatgctaag
     3001 caacatgttc ttattcttac ctcgtaatat ttaacgcttt tcgcaaaata aacggacaga
     3061 cgattaaaaa tgcatattcc gggctaaata tcgaaccggt aaaaaacagc gataccggag
     3121 taaaaagata taattgcacg cgaaaattgc ggttatcacc aaaagcgttg atcatcattt
     3181 ttatcacttt ccccatgtac cacagcgata aaaacaccgc gaaccaggaa aaataaataa
     3241 tcagcagata caaaccattg tctatggttt ttccgacatc cgcaccgtta aatattccga
     3301 atgatgcgac atattcataa agtgagccaa atctgactac accgtcaata tgggtcaaag
     3361 aataaccgac catcaccagc ggaccgacaa tacgataata cgaagacgat ccttccgtac
     3421 ctaaatcgcc cagacgggtg gaaatataag gaaacgcgat taccacacca accaggaata
     3481 cagccagaga aatcaatgct aacggtaact ttttcttaat cgcctcttta ttcagatatt
     3541 ggaatgccca ctccagcaaa taaaacagga taaaggtcat aacccctgaa aacgatccgg
     3601 ataatattat ccctgcgaga atcatagcat ctgttttagg cgttttgata ccaaactgtt
     3661 tgatgctgag ccaaattgag attaatgcca gagcgaaaaa tgccggttcg aaataaagtg
     3721 ctgtcgtgcg cttgccgccg aatttaatga aattcagtac atagctgttg ctataaatca
     3781 gatatttcga aatcgactcc ataatactgc tgccaccggt gaggataatt tgcgccatct
     3841 ccaccgccgc cagcgccacc accagcccga ccaccagata aaagaaacgt aatatcttgc
     3901 gatggttgtg tggcgaaatc gttttaaagc gaatactcca caccatgcca ataatgatca
     3961 caatatagac aaacagcatg gttgaagtga cgtatttact ggcatccaac gactgaccaa
     4021 acagatagtt gaacgccgtg agccccgcgc caacccctaa ggcaatcatc aattttttaa
     4081 cgctgatgcg ttctaaaaac agcagtaaca agacaggtaa aaaagtgacg atagtgatgg
     4141 ggaaactttc accaagctgg gcgattttga cgttaaccag caggtagatc agcggcagca
     4201 gcaggtagct acagattctg atagaagttg acatactcct ccagcatctg ttgtccactg
     4261 taggcggcgc ggctacgttg gctgaacccg gccaacgtgg taccaaatat cgcctgcgcg
     4321 atttccggtt tgcttaattg caccagttgc agcacctctt cttcgctgac ggttttaccg
     4381 ccggattttt gcaacacttc acgcgccgcg tcgctatggg tggcaatcac cggcacgcca
     4441 atcgatagcg cctcacacaa aatcagcggg tagttatcga cgcgagaact gaacaccagc
     4501 gcatccatct gattgagcgc gctcatcaac ttgcgcttgt cagtttcaaa gccgtgatta
     4561 accacgttgc cagcggtgaa cggcgagaac ttaccaaagg tatgcagttc aattttgtcg
     4621 cccagcgcca tcatctcacg caccagttgc tggttagtct tgccgtcgta acgcaggtca
     4681 tgcgccacca ccgcgatttt cggcttaccc tgggtttcgc gcaccggagg cagatccgcc
     4741 agaatcgctt cggttgccat atcaataccg ttattgataa tccggcaacg ccctggaccg
     4801 tacaggctat tgaaagcgtc agccacatgc tggctgggtg aaataaactg acagcccagc
     4861 gccagcatct cacggaataa ctggcgtttg cccgccacca gctggtgcgc ccgatcaatc
     4921 ttcaccggcg gataattatt taaggtcggg catttctggc agcccgtttt ccagccttca
     4981 caaccgtcgg taaaggcgca gcgcccggta acgctccagt ggtcatgcag cgtccagacc
     5041 agagtgacat ccggtttgtg gtttttcacc ttttcgcaaa agcgcaccac gctcttaaga
     5101 tttagccagt agctgtgcag cacatgaaaa tgcaggacca ccggacccgg tgtgcgagta
     5161 atggtgcgat ataactcatt gaaattgcca aacagatcgc gattaaacag acgaaacagg
     5221 gcaatattcg ccattgcggt catccgcggc gtatgtttga ttacctgcgg atagttctga
     5281 tggctgacgc tctctttgcc gcctttgccg tagccgtaga caaaatgtga cgccagcccc
     5341 tgttgcagcg cacgctggtg gagatctaac gccacacctg ccgccccgcc ttccgccagt
     5401 cgcacattaa attgcagaat attcatttaa ttaccttcac tcgcgctttt tctcccacca
     5461 ccagcgcgtt gtccgggaca gagtcgagca ccacgctgcc cgcgcccacg gtgacgttgt
     5521 taccaagcgt gatatcacca agaataatga cgttggcacc gagttcgacg ccgttgccaa
     5581 tgtgtggaca tgccatgttt tcggcaccac gattgccgat agtgacgccg tggcgaatgg
     5641 taaaatcatc ccccgctact acgtttttat tgatcactac ggcgtaaccg tgatggatag
     5701 taaagcggcg gccaatggtc gcggcggcct ggatttcata accgaaaaag cattcggtga
     5761 taatgcgata cagcaccagc agcggggccg cccacagatt gttgaggacg ttctttttgc
     5821 gccacaccga acaaaaatga gcaacacgat aggcaagaac catgcagcac gggcgtaaac
     5881 tccagctgtt ggcacgcaga tcttccagca tctttagcgc ccccgaatac catcagccag
     5941 acgtttgccg ttacgcaccg acagcagcgt cagcaaagtg cgccaggtca tacgtttatt
     6001 gcggatctgg tagagggtaa acagctgata ttttttgctt gcacggtcaa atttatcttt
     6061 gtgcttgcgg taaaagtgga agtacccgga gaattttttc ggtgacgagg tgatctgcat
     6121 ttcaccgtga ttgatatgca ggatctgcgt cgcctcttcc actttccacg gttcgccata
     6181 ctccaccacc atccgcagga agatgtcgta atcctgtgct gctttcagtt cggtatcgaa
     6241 caagcactct ttgaaacgcc acgcccaggt aaaaacctga ttgccaatga tattgcgttt
     6301 atagaacagg cggcgtgagt acggcgattt gggatacagt ggtaagcttg ccggttggga
     6361 ataaacttct ccctggcata cgtagtcgtt agcgtacaaa aaagcgtgag tgaccagttg
     6421 ctgtttatgg gcgaggaaga cgctcaggcg gttgggcgtc cattcatcat cgtcatcgat
     6481 cccggtaatg tattcccctt gcgccagcat aatcgcctgg ttacgtaccg cgcacgcccc
     6541 gctgttaatg tcgttgtgaa tgtaggtaat acgcggatcg ttgagggcag tgacgtactg
     6601 ttgcagttgt tcccaggagg tggagcaatc atccacgatg atcatctccc agttgctgta
     6661 gtcctggcgc aggaccgatt ttatcgcccg aatcgccagt tgttggcggt tccaggtcgg
     6721 catatagatt gaaatcagcg gattgtcttt cataggttgt tctcccggag tgtcgagatg
     6781 ttctgccggt ttataagctt gtttacgggt tctgttgccg gatgcggcgt gagcgcctta
     6841 tccggcctac ggagctggtt tgcttgccgg atgcggcgtg aaggtaggtc ggataagatg
     6901 cgtcagcatc gcatccgaca gcggatgtcg ttatttcgca tccgacttat attcgtattc
     6961 gtaatagcca taatcctgat acgcgctggc gcggcggaag atggagttca gaatcacccc
     7021 tttcaccgga ataccatttt gctcaaagcg gctcagactg gtttccactt ctttcaatgt
     7081 gttgaccgca taacgcgcca ccattaacgt ggttccaaca tgacgaccaa caattgccgc
     7141 atcggtcact gccagaatcg gcggcgtatc aatcaacacc aggtcatagt ttttactcgc
     7201 ccagttcacc agttcggcaa agcgttcgct catcaacagt tcagaaggat ttggcggtac
     7261 ctgaccgcgc gggatcaggt caaatttggc aatagaggtc ggtttagctg cggtagtaat
     7321 atcgccctgg ccaatcagaa tttccgacag gccattgacg ttattggtac ccaacagctc
     7381 gtgggtgtag cctttgcgca tatcgcagtc gatcaacaac acccgtttat tggtctgact
     7441 gatcaccgcc gccaggttgg cgcagacaaa ggttttaccg attgacgggc tcacccctgt
     7501 catcatcaac acattgttct gcgcctgcat catcgcgaag tgcagactgg tacgcagact
     7561 gcggatggct tcaatcgcca gatcggttgg attccccacc gccagtagct ggctctgttt
     7621 atagcgttta atccctttga tggttttgac gctatcgcgc gctttctgcc attccgacaa
     7681 cgggatgctg gcatagacgc tgataccatg ttcttccagc acctgcgggc tttcgatgcc
     7741 gcgattaaac aacgagcgca gcagcacacc cacgatagag agcatcaggc cgaggataat
     7801 tgcgccgagg ataatcagtc ctttcttcgg tttcagcacg ccaggctggg tgattgccgg
     7861 gtcaacaatg cgcacatcgc cgacggtgct ggcttcggtg attttcagct cctgctcttt
     7921 attcagcagt tgcatataga cctgctgacc agactcgaca tcgcgggtca gacggacaat
     7981 ttcctgctgg gttttcggca tcgccgttac gcgaccatta agtttggctt tttcgtcttc
     8041 cagcgcctga cgtttctcca gcagcgtgcg gtacgcgggg tgaactttgg tgtacagctt
     8101 ggagatttcc gcctctttaa aggtcagttc gttcaactgc gcgtcgatgt tcaccatcga
     8161 atcgagcacc gctttcgctt ccagcggcag atcaacagaa tctttatcct gacggaaggc
     8221 attcagtttg ttttcggcaa catcgaggcg gctacgtact tccggtaact gttgcgcgag
     8281 gaaagcgagg cttttcgacg cttccgccga tttgcgctca atattttgtt cctgatagtt
     8341 acgggcgatg ctgttaagaa tgtcgcggat ctgttcgcga tcttcaccgg tataagtcag
     8401 gctcagtacg cctgcgtctt tgccgttctc cgttaccgtc aggctgtttt gcagttggtt
     8461 gatcatcccc aacgtggagt atttggtgac ggtaaactca ctgcccgggc gggcgtgaat
     8521 ggcttcaacc atcagcgtga cgccttcttt tttcagcatc tggcccgctt gcccacgggc
     8581 gctaaagccg ccatcactgc tcagagtgta gtttttgtca tccagcacat taagcgtaaa
     8641 cacctggtcc gccatctctt tcgggcggtt aaaggtggtc actttcaccg tctcgttctg
     8701 acgtcccatc aggcgatccc aacccgcacc gaaaatcggg aacgtgtttt tgctcaccgc
     8761 aatatcgagg tctagatcgt ccaccgtttt accaagcacc agacgcgaac gaatcaactg
     8821 gatctcggcg tccgatgcag gcggtttgtt ggctaatgcc gagccaatgt cctgcactaa
     8881 cgaattgccg ctgttttgtt cgatttgtac cagtgcgtcg gcactataaa tcggcgtggc
     8941 gaagaaagtg taaaccacag cacagagggc aaatacagcg gtgatgccaa tcacccacca
     9001 gcgcgcttca atgacggtgc cgaccaggcg accaatatcg atttcatcac tgcccgttac
     9061 cggagcggca tgttgtttta ctttttctgt cattcttata cctgctctgc gttcaatgcc
     9121 tgcgcccact ggcgggcaga ccgttcaagt aatgtgtaca ccgctgcaaa cgtttcccgg
     9181 cttttgcgat acggatcggg gatttcacat tcgttatccc agtgaccaaa cagcatcact
     9241 ttgccgcgca tctcaggtgc catctcgcat aagcgttcga tatggcgctt ttccatggtc
     9301 aaaatcaggt catagttgcg acacaggcgg cggctgattt gccgtgcaca gtgaccttcc
     9361 agagacagtt gatgttctgc ggcgacgctg atagcggtag gatcagcgcc cttaccgacc
     9421 agcgcgccga gtccagcgga ctccactttc agctccgggt gataacgttg cagtaagcgt
     9481 tccgccgtcg gggaacggca aatattgccg acacagacaa ctaagatgtt gttaaacatg
     9541 acgattacca gttatgaatg tcgctggctg tatccgtcat gtaacggaca ccgctaatag
     9601 ttggcagcaa ctgattgatc agacggttcc agcgggaaac cggggcggtg gtaacataca
     9661 ccacgtcata aggttgcagg cggaattctg tcgccatcac cagcgacgtc gcatcggaca
     9721 tatccagctg gtagatattg gcaatcttgc cattacgccc gccctcgcct ttcagcggac
     9781 gaatgacaaa gatgccgctg gcgttggagg tggtcatgtc gataccttcg gcattgccca
     9841 gggcttcagt cagggtcatg ccgctaaagt ccattttcag ggtgctctgt ttcttcactt
     9901 cacccatcac aaacactttc agatcatcat tacgcggcac gtagagaata tcgccgggat
     9961 aaagcagtcg gttctgattg aggtcgccgt tttgcatcag cgcctgcaaa gaaatgcgtt
    10021 cttcacgacc attgtgtgtt agcaccacgt tgcgccagtc agcggtgtcg gtcaggccac
    10081 ctgcggcgtt gatggcgtcg agaatggtca gtggtacgtt ggtgatcgcc tgttgaccgg
    10141 atttattcac ctgtccggag atataggcct tttgtgagcg gaaggcggcg atattaacgt
    10201 ccacctgcgg gtcagcgatg tacgtcgcta agcgcccggt aatatcactg cggatttcag
    10261 cgagcgtttt cccgactacg tgaaccttgc cgatatacgg gtaaaacata gtgccgtcag
    10321 gctgtaccca gttgccggtg tcactggagc tgcggtactg accggctggc gtggtgagtt
    10381 ccgggtgatc ccatacggtg acattcagaa cgtcgcccgg cccgacgcga tactggtaat
    10441 tcgcgatctc actttccagc gtcatattgg ggcgcgctac gttgggtcgt ggacgtaatt
    10501 gatcaatcag gcgcggggtc aacggataaa cattcaccat tttgtcgaga tcgaaatcag
    10561 cgtcctgctg tttgatgacg tctttgccca tcgtcgacat attgctgccc ggaagtactg
    10621 tgcaaccgct tatcaaggtt actgacacca ataatggcat caatttcatt ttggatttca
    10681 tcattgttta tttatcactt tggcagagta attatcctgt gcactattaa tagcaatgtc
    10741 gccatgcaca tttaccttgc agttaattga atacaaattt aactggcatc agtcctaaaa
    10801 aaattgattt catccgcagg ctattgacag aataattcag actggtcttt caggcatcca
    10861 gacacgctac cgcccctggc tttttagcta ccaatacact gatttagctt aatttttcaa
    10921 accctctctg catacaatcg ttgatgagaa agggttattg cggaaattaa cttccgaata
    10981 taaggtgaca ttatggtaat tgaatattgg ctctccaata atgcaggagg aagtgttaca
    11041 gctaacggaa tagcaggcaa ggtaacaatt cggcaattgg
//
LOCUS       DAZGSP010000144        10785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZGSP010000144 DAZGSP010000000
VERSION     DAZGSP010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.145"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1816)
                     /gene="selB"
                     /locus_tag="ACHK74_004552"
     CDS             complement(<1..1816)
                     /gene="selB"
                     /locus_tag="ACHK74_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFP0223255.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLL"
     gene            complement(1813..3204)
                     /gene="selA"
                     /locus_tag="ACHK74_004553"
     CDS             complement(1813..3204)
                     /gene="selA"
                     /locus_tag="ACHK74_004553"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFP0223256.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3302..3910)
                     /locus_tag="ACHK74_004554"
     CDS             complement(3302..3910)
                     /locus_tag="ACHK74_004554"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFP0223257.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(3981..5117)
                     /locus_tag="ACHK74_004555"
     CDS             complement(3981..5117)
                     /locus_tag="ACHK74_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP0223258.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5120..5482)
                     /locus_tag="ACHK74_004556"
     CDS             complement(5120..5482)
                     /locus_tag="ACHK74_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFP0223259.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQSERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6019..7932
                     /gene="mtlA"
                     /locus_tag="ACHK74_004557"
     CDS             6019..7932
                     /gene="mtlA"
                     /locus_tag="ACHK74_004557"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFP0223260.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETMAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTANEEKVKDSLKDSFDDSSANLFKLGTENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            8027..9175
                     /gene="mtlD"
                     /locus_tag="ACHK74_004558"
     CDS             8027..9175
                     /gene="mtlD"
                     /locus_tag="ACHK74_004558"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP0223261.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9175..9762
                     /gene="mtlR"
                     /locus_tag="ACHK74_004559"
     CDS             9175..9762
                     /gene="mtlR"
                     /locus_tag="ACHK74_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFP0223262.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(9774..9983)
                     /gene="yibT"
                     /locus_tag="ACHK74_004560"
     CDS             complement(9774..9983)
                     /gene="yibT"
                     /locus_tag="ACHK74_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFP0223263.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            10268..10630
                     /locus_tag="ACHK74_004561"
     CDS             10268..10630
                     /locus_tag="ACHK74_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFP0223264.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         2595 a         2912 c         2771 g         2507 t
ORIGIN      
        1 gcaataaatg atcatttcca cgacgacgcg taaagccaat gcggtcaaaa tattccagaa
       61 tctgaattgc cagctttcgg cctacgccta agcgatcgcg gaaatccgcc gcgcaggttg
      121 aaccacactc ctgatcgaga tcgcggatca tattggcaaa ttcgacaatc cgatcgttac
      181 ggtaataacg atctttgacg atcgcggtaa ttattccttg ctgcgccgcc tggcgtagcg
      241 tcaggcgcat tgcctgctcg tcggtacccg tctcttttgc cagatcacgc acccaccacg
      301 gctcgtcacc aaacagcggc tctgcttttt gccagatggc ctgctgctct tcgctgaagc
      361 ccgctttgtg atccggcaga tgcagccaac cgtgatgact gaggatgtcg ccgctttcgc
      421 gcatcttttc aatcagcaac agtactagcg cttcatcttc cattggcaac gccatacgtc
      481 gcagacgttc gcgcccaggc ccaggttcat cgcgatgttg ctcatgataa gtcgctaatg
      541 tgtcgagaat tttccgttgc cagcgagcgg caaccggcgc attcaacaag ctataaccag
      601 cctgaatata gccaggctgt tgcagcaatt cgcgcatccc ttcgccgttg agctggcgcg
      661 cccaggcgaa atccgcaagg ttaaccgcgc cgcgttccag atgaacagat aacgcatcgg
      721 catcgctctg cgcccgtgca agagacgcca gccattgcag atattccggc ttacgtttac
      781 cgcgacgcgg cgggttaagc atcaccacac gcgcccccgc cagcgtgttg cgggccgaga
      841 tatcgcgcag cactaagcga tcgttatccg ccagccacag cggcgtgtcg aacaccagct
      901 cggcaaggtt atcttccagc agcgaaacgc gtcccgtaac gtggctggcg gcgtggtgaa
      961 tatgcagcgg ctgccactgg gtcagcggtg tatgggtttg aagctcgaca atcacccgtg
     1021 tgaacggctc tggcggcaca tcggcaagca gccagtcgcc acggttaatc tgctcttttt
     1081 ccgcatcacc cgcgatgtta agcgcgatac gctgcccggc atgggcggtt tctgttggct
     1141 ggttttgcgc atgcagcgca cgtacacgca tcggtttatt tacaccagtc agccagagtg
     1201 aatcgcctac cttcacttcc ccgcttaacg ccgtaccggt gacgaccagc ccggcacctt
     1261 ttacggtaaa tgcgcggtca atagcgaggc ggaaactatg ttggctggcg tgctcgcgtt
     1321 ccggcaactg aagcagatgc tcgcgcaggg catcaattcc ccgaccttcg gttgctgcgg
     1381 tgataaacag ttttgcctca gcaaaaccgt attcccgcag cacctccttt acctggcgtt
     1441 caacctcatt aacacgcgct tcgtccacgc gatcggcttt ggtcagcgcc actgtcagca
     1501 tcgggttacc ggtcagctgc aaaatcgcca gatgctcacg ggtctgtgcc atcacgccgt
     1561 catcgcacgc caccaccaac agcgcgtgat cgataccacc aacgcccgcc agcatgttgg
     1621 aaagaaactt ttcatgaccg ggtacgtcga taaaaccagg cacgcgacca tccggctgcg
     1681 gccagtaggc atagccgaga tcgatggtca tgccgcgctt tttttcttcc ggcagacggt
     1741 cagcatttac gccagtaatc gcctgcaata aggttgtttt gccgtggtca acgtgtccgg
     1801 cagtcgcaat aatcatttca acaacatctc caaaaaccgt tgctcatctt caaggcagcg
     1861 taaatccagc cacaatcgtc cgtcataaat acgaccaatc accggcactg gcaattcacg
     1921 ccagcgggcg gctaatgact caaggtggct accgcgtcca tcatggggtg taaacgttaa
     1981 tgccgcgctc ggcaggcgat caaccggcag cgaaccactg ccaatctgcg aaagacatgg
     2041 cataacctgt accgcaaact ccgcgccgta atgtgcggca aggggggcct gtaaacgttg
     2101 tgcctggatt tgaatgacct ctgcgctgcg ggtaagcagg cgcagggtcg gtaatttttc
     2161 actcagagct tcagggtgta aataaagacg caacgtggct tccagcgccg cgagggtcat
     2221 tttatccgcg cgtaatgcac gcttcagcgg gtggctttgc aggcgggcga tcatctcttt
     2281 tttaccaaca ataattcctg cctgcggccc gcctaacaac ttgtcgccgg agaaactcac
     2341 cagactgacg cccgccgcaa tcaactcctg cggcattggc tctttcggca aaccgtactg
     2401 gctaagatcg accagcgagc cactgcctaa atcagtcact acgggaacat ccagctcttt
     2461 gccgagcgcc accagttccg cttcatctat cgctttggtg aacccctgaa tgctgtagtt
     2521 actggtatgt actttcatca acagtgcggt attttcattc accgcctgac gataatcatt
     2581 cgcgtgcgtg cggttggtgg tccctacttc gtgtagggtg cagcctgcct gacgcataac
     2641 atcgggaata cgaaacgcgc cgccaatctc caccagttcg ccgcgagaaa ccaccacctc
     2701 tttcccgctg gcagtggccg ccaacatcaa taacaccgcc gccgcattgt tattaacgat
     2761 acaggcatct tccgcccccg taatgcggca cagcaactgc gccagcgcac gatcgcgatg
     2821 tccgcgtcca gcgtcgtcca gatcgtactc gagggtcact ggcgaacgca tagcctgcgc
     2881 aacggcttcc accgcggctt ccgcctgtaa agctcgccca aggttggtat gcagcacggt
     2941 tcccgtcagg ttgatcaccg gacgcagcgc gctctgcgct tctttcgtca accgggcatc
     3001 gacttcttgc gcccagtttt cacaccacgc aggcagcgtc tggctgccac gaatcacttc
     3061 tcgcgcttcg tcgagcatct gacgcaacaa ttccaccacg cgggtgtgac cataagtatc
     3121 acgcaaagaa aggaaggagc tatcgcgcaa taagcgatca atagccggaa gttgactata
     3181 gagggaacgc gtttcggttg tcataggaaa cctggctgat caaggccctc tcacacggag
     3241 aagggcgttt aacataacca cggattgtaa cgtgagatgg gtcaggagga catatcgcgc
     3301 atcaagcctt tggcggttcg gtgcgagcaa aactttcgcg gctaaacagg ttttcgacca
     3361 gtttgactaa gtgtgggcga tcgacacacc agcctggcgc aacgcggcgg aaattgagat
     3421 atccgacggc acaggcaatg gcgatagtcg ccagattgac cgtatcggtt ttgagtgtgc
     3481 catcgaccag atatccttcc agcacatcca gactgcggtt gattttctcc cgctggcgta
     3541 acaattcatc ttcagactgc tgcgccgctg gacgcgcctg ttcacgcacc gataccagcc
     3601 cggcatccat aatgccatcc gccagtgcct caattttgcg cacccgcaac gactccagcg
     3661 gatcgcgcgg caacatcgcc ggagcgacat tcattaattc aatgtattca gcgatgatcg
     3721 gcgaatcaaa ccagcattcg ccctcttcgg tcaccagcac cggcactttt cctaacgggt
     3781 taaattgcgc cacgccgttg tccgcgttat agggcagttc attaataaat tcgaaagtta
     3841 tgcccttttc taacaacaga atagaaagtt tgcgtacaaa cgggctggtg tagctaccga
     3901 cgagtttcat gccgagtcct ttgtgcgagg aaaaatatca gtatggctca ggctcacgca
     3961 aaacacaggt gatataagtc tcaatgatcc aaataaagat aatgcatcca gctggtcatt
     4021 cttagcagca ctttccgcat caccgaaaca tggctgaaat ggtcggagta aactgccacc
     4081 tgggcgtaga tgccgtcggg taaggcatcg atatcatcgt taggatccag ttcaatggtt
     4141 cccagcacac cgtccgtgcc gggcacgacc gttaatgatt gcaatacccc ctgcgcctga
     4201 taagaaccgc ctggcacgac aggtaaaata ctggtcagtt tgccgtggaa cacctgccca
     4261 ggtagcgcgt taaacaccac ttccgcatca tcgccaggtt tcagacgtaa cagcgagttt
     4321 tgccgaaatt gggcgacaat ttgccgtttt tgctcgggga tgaaaaccat caccggacgc
     4381 aacggcaagg cagctgcgta tgtacctggg cggatcagta cctgagtaac gtagccattg
     4441 ctcggcgcgc gaatgacagt ctgctcaagg ttatattttg cttcagtaag ttgcgctctt
     4501 aagctcacaa tctgcgattg ctcgccgtta accatactgt cgagctggct ctggatctgc
     4561 gcctgctccg ccaccgagcc tttcaccagc gcatcctgcg caaggaaatt ttgccgcgca
     4621 tcgtcgatgt cacgttccga gaacggattc accgccgcct ggctgccttt caggtaacgt
     4681 tgataatttt taaacagacg gtcgcgctcc gctgaaacct gggtggtgtt ggcctgcgct
     4741 tcagtgagct gcgcgcgcag cgtctttata ttatgcgtcg ccgtcatcag gtcagcctga
     4801 agtctgtcaa ctcgcgcctg gtaacgaacc gggtcgagct taaaaagcac ctcgcccttt
     4861 tgaataagct gattattctt gtcagtgact tcagtaacaa ttcccgtcac ctgtggcgtg
     4921 atagggatcg ctatcactgc cttttgcgcg gtaaaagtgt aagggtggtt gtagttcatc
     4981 aacaaaatca aaccactcac cagaaacacg cctcccagcg ccgccgtcgc cagcgtccac
     5041 tgatttaccg gaatgcggaa gattttaaag accgcccacg ccagcgccac gtaagttaaa
     5101 acaatcaata gatccataat tagatctccg ggaacgtgga atagtcagtc tttttctcgg
     5161 cggcgagttg atgctccagc cgggaaattc ggtcagaaag tgaggcgatt tccgggtcag
     5221 tcgctttctc ctgcgacgca acatgtgact gcatacccca gccacgctcc gattgataga
     5281 gcgtcgccca gatccacaga aacggccaga taacatgcag agtaaacagg ctcacccagc
     5341 ccgccgtatg aatagcgtcg gcatggggat ggttgcgctt tttggcaatc agataaggga
     5401 tgtcatgtat cgcgatgatt ccataaaaaa tcaccagaaa gacgaagatc agcactccca
     5461 acgcgaaata gtttagaaac atatttgcct cggattcacg tttatcagtg ttgttttttg
     5521 gctggcagcc agaagggagt caggctgata ttttgacaat aatccgggtt cgcgattctc
     5581 gccataacac caaagaataa tttttagagg tgatgagttg cttagttaca taacgattgt
     5641 atgacgaagg cataacatgc tgtagatcac atcaggtgaa cgccgtaaga aaatatcttg
     5701 tgattcagat cacaaagatt caacaaacca tcaaaacaaa aatgtgacat tactcacact
     5761 taaatgccat ttttagcgaa aatcgccgtc ttgttgcttt tttacacaag cgttttgtga
     5821 tgagtatcac gtcaattacc tctctacccc ctatatttat gtgactgata tcacacaaaa
     5881 ggccgtcgac tggacagtta accgattcag tgccagattt cgcagtatct acaaggtccg
     5941 gctacctctg ccgccacatt aacaaaaaac ctcgggcttc cagcctgcgc gacagcaaac
     6001 ataagaaggg gtgtttttat gtcatccgat attaagatca aagtgcaaag ctttggtcgt
     6061 ttcctcagca acatggtgat gccaaatatc ggcgcgttta tcgcgtgggg tatcatcacc
     6121 gcgttattta ttccaacagg gtggttaccg aacgagacga tggcgaagct ggtcgggccg
     6181 atgatcactt atctcctgcc gctgctgatc ggttataccg gtggtaagct ggtaggcggc
     6241 gaacgtggcg gcgtagtcgg tgccatcacc accatgggcg ttatcgtcgg cgcagatatg
     6301 ccgatgttcc tcggttcgat gattgcaggt ccgctgggcg gctggtgcat taagcacttc
     6361 gaccgctggg tagacggtaa gatcaaatcc ggttttgaga tgctggtgaa taacttctcc
     6421 gcaggcatca tcgggatgat cctcgccatt ctggcatttc tcggcattgg cccgattgtt
     6481 gaagccctgt ccaaaatgct ggctgcgggc gttaacttca tggttgtcca tgacatgctg
     6541 ccgctggcgt ctatctttgt tgaaccggcg aaaatcctgt tcctcaacaa cgccattaac
     6601 cacggtatct tctcgccgct gggtattcag cagtcccatg aactgggtaa atccatcttc
     6661 ttcctgattg aagctaaccc aggtccgggt atgggcgtgc tgctggcgta catgttcttt
     6721 ggtcgtggta gcgctaaaca gtctgcgggc ggtgcggcaa tcatccactt cctgggtggt
     6781 atccacgaaa tttacttccc gtatgtgctg atgaatccgc gtctgatcct ggccgttatc
     6841 ctcggcggta tgactggcgt gttcacgctg actatcctgg gcggtggtct ggtttctccg
     6901 gcatctccgg gttctatcct tgctgtactg gcgatgacac caaaaggtgc ttatttcgct
     6961 aacatcgcgg gtgtgtgtgc ggcgatggca gtctccttcg ttgtctctgc tattttgctg
     7021 aaaaccagca aagtgaaaga agaagatgat attgaagcag caactcgtcg tatgcaggac
     7081 atgaaagctg agtctaaagg tgcatctccg ctgtctgcgg gcgatgtaac taacgacctg
     7141 agccacgtac gtaaaatcat cgttgcctgt gacgccggta tggggtccag tgcgatgggc
     7201 gcaggcgtgc tgcgtaagaa aattcaggat gcaggtctgt cgcagatttc ggttactaac
     7261 agcgcgatca acaacctgcc accagatgtg gacctggtca tcactcaccg tgacctgacc
     7321 gaacgcgcta tgcgccaggt tccgcaggca cagcatattt cgctgaccaa cttcctcgac
     7381 agcggcctgt acaccagcct gaccgaacgt ctggttgctg cccaacgcca cacggcaaac
     7441 gaagagaaag taaaagacag cctgaaagac agctttgacg attccagtgc taacctgttc
     7501 aagctaggca cggagaacat cttcctcggt cgcaaagcgg ccaccaaaga agaagcgatt
     7561 cgttttgctg gcgagcagct ggtgaaaggc ggttacgttg aaccggagta cgttcaggcg
     7621 atgctggatc gtgaaaaact gaccccgact tatctgggtg agtctatcgc ggtgccacac
     7681 ggtacggttg aagcgaaaga tcgcgtactg aaaacgggcg tggtgttctg ccagtacccg
     7741 gaaggcgtgc gcttcggtga agaagaagat gacattgccc gtctggtgat tggtattgct
     7801 gcccgtaaca acgagcacat tcaggttatc accagcctga ccaatgcact ggatgatgag
     7861 tccgtcatcg agcgtctggc acacaccacc agcgtggatg aagtgctgga actgctggca
     7921 ggtcgtaagt aatccaatcc taccctctcc tcacggagag ggtttgggtg agggaaaagc
     7981 ctcaccccag ccctctcggg taaaaacatt gatgaaggtt aatactatga aagcattaca
     8041 ttttggcgca ggtaatatcg gtcgtggctt tatcggtaaa ctgctggcag acgcgggtat
     8101 ccaactgacg tttgccgatg tcaatcaggt ggtacttgat gccctgaatg cccgtcatag
     8161 ctatcaggtt catgtggtcg gtgaaaccga gcaggtagat accgtttccg gcgtcgatgc
     8221 tgtcagcagc attggtgatg atgtcgttga tctgattgct caggttgatt tagtcactac
     8281 cgccgttggc ccggttgtgc tggaacgtat tgctccggct atcgccaaag ggctggtgaa
     8341 acgtaaagaa caaggtaatg aatccccgct gaacatcatc gcctgtgaaa acatggtacg
     8401 cggtaccacg cagctgaaag gccatgtgat gaacgccctg ccggaagacg ccaaagcgtg
     8461 ggtagaagaa cacgttggct ttgtcgattc cgccgttgac cgcatcgtac cgccttcggc
     8521 ttcggcaact aacgatccgc tggaagtgac ggtagaaact ttcagcgaat ggattgtcga
     8581 taaaacgcag ttcaaaggcg cactgccgaa catcccaggc atggagttaa ccgacaacct
     8641 gatggcattt gtcgaacgta aactcttcac cctgaacacg ggtcatgcta taaccgcgta
     8701 cctcggaaaa ctggccggtc atcagaccat tcgtgacgct attctcgacg agaaaatccg
     8761 cgcggtggta aaaggtgcga tggaagaaag tggtgcggta ctgatcaagc gctacggctt
     8821 tgacgcagac aaacatgcgg cgtacatcca gaaaatcctc ggtcgttttg agaacccgta
     8881 tctgaaagat gatgtagagc gcgtaggccg tcagccgctg cgtaaactga gtgctggcga
     8941 ccgtctgatc aagccactgc tcggtacgct ggaatacagc cttccgcaca agaatctgat
     9001 tcaggggatt gctggtgcaa tgcacttccg cagtgaagat gatccgcagg ctcaggaact
     9061 ggcagcactg atcgctgaca aaggtccgca ggcggcgctg gcacagattt ccggtcttga
     9121 tgccaacagc gaggttgtat ccgaggcggt aaccgcttat aaagcaatgc aataatggtg
     9181 gaccaggcgc aggacaccct gcgcccgaat aacagattgt cagatatgca ggcaacaatg
     9241 gaacaaaccc aggcctttga aaaccgtgtg cttgagcgtc tgaatgctgg caaaaccgtg
     9301 cgaagctttc tgatcaccgc cgtcgagctt ttgaccgagg cggtaaatct tctggtgctt
     9361 caggtattcc gcaaagacga ttacgcggtg aagtatgctg tagaaccgtt actcgacggc
     9421 gacggtccgc tgggcgatct ttctgtgcgt ttaaaactca tttacgggtt gggcgtcatt
     9481 aaccgccagg aatacgaaga tgcggaactg ctgatggcat tgcgtgaaga actaaatcac
     9541 gacggcaacg agtacgcctt taccgacgac gaaatacttg gaccctttgg tgaactgcac
     9601 tgcgtggcgg cgttaccacc gccgccacag tttgaaccag cagactccag tttgtatgca
     9661 atgcaaattc agcgctatca acaggctgtg cgatcaacaa tggtcctttc actgactgag
     9721 ctgatttcca gaatcagctt aaaaaaagcc tttcaaaagt aagcaacgtc tgcttactgc
     9781 ccctctacct gcttcggccg ataaagccga cgataatact ccagacgttg tagatataac
     9841 ggcacgcttt catcgggtat tccggacgga atcgcgttac ggggaggaag ttttttcaga
     9901 tactcccgga acgcctggct tgatgccatg aaatctacgg ctttatcgat aagaagcgga
     9961 acgttttcac ctaatttgcc catgatgata tctccgtatt accccgcgcc gggaatgcgc
    10021 ggccgccaat tttagtttag gttcccggta aagatgacat taaggaaaac gtgctgaatc
    10081 ctcaataatc atccgctgct gtttattatc ttttttctat agatttctta attaatcaat
    10141 aaattgcgta gtttttaagc atgttaatga ttacgttaca gaactattgc aatgtcatca
    10201 gttgtgaaca aagcacctgg tcgcgcatac taggggctat aaatttatct ttatcagaag
    10261 ccatcacatg aaagaagtcg aaaaaaacga aatcaaacgt ctcagcgatc gtctggacgc
    10321 catccgccac cagcaggccg atctgtcgct ggttgaagcc gcagacaaat atgccgagct
    10381 ggaaaaagag aaagccacgc tggaagcaga aattgctcgc ctgcgtgaag ttcatagcca
    10441 aaaactgagt aaagaagcac agaagctgat gaagatgccg tttcagcgcg caattaccaa
    10501 aaaagagcag gctgatatgg gcaagctgaa gaaaagtgtt cgcggactgg tggttgtgca
    10561 cccaatgacc gcgctgggcc gtgaaatggg cctgcaggag atgactggat tctctaagac
    10621 tgcgttttaa gcgcctcaga cgactggttc gatcatatca acccgcatct ggcttgcctt
    10681 agctggtcaa tgcggggact ctcctgtcta ctcgcctaat taccaaacaa ttcactccct
    10741 ttacttcgat ttaccgctgg ttgatattct gaaaatgcat tttta
//
LOCUS       DAZGSP010000145        10726 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZGSP010000145 DAZGSP010000000
VERSION     DAZGSP010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.146"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            264..605
                     /locus_tag="ACHK74_004562"
     CDS             264..605
                     /locus_tag="ACHK74_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFP0223265.1"
                     /translation="MFSFPASSCRAGRLAGAAGMLFLLSFALPAQAAEKDELATVMRQ
                     LDHVQAALERARTASLQASSQGERYFFDYLRARADLQTIRDGIDHYLAPSRAQPRSSA
                     AVTGDYRRERP"
     gene            605..847
                     /locus_tag="ACHK74_004563"
     CDS             605..847
                     /locus_tag="ACHK74_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFP0223266.1"
                     /translation="MAMTALQNTAFRAASGEVDLSVLHLITLGFLLAVVFLWAAWSLT
                     DAWAGWRSGDVRDGVFGWFMFRLVLLLICSIWMFAG"
     gene            924..1238
                     /locus_tag="ACHK74_004564"
     CDS             924..1238
                     /locus_tag="ACHK74_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFP0223267.1"
                     /translation="MAVFLCTGITPALADLPQIEPPTSGGGGGLFGQIKGYLQDGVVL
                     FGLVLSAVAFVKVAEAALSTFSEVRDGKAGWGQFGAIVVVGVVLLVAIIWLLGKSASI
                     IL"
     gene            1251..1613
                     /locus_tag="ACHK74_004565"
     CDS             1251..1613
                     /locus_tag="ACHK74_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFP0223268.1"
                     /translation="MQTIRFLPDRLNVEPAVFRGFTTPELGLAALTGAVAGVIWPLPL
                     IPLLGWVIIPTGMLLTPLLLVWFGGVWISRLKRGKPDNWLWQHLAKKSSAWGGGRKTF
                     IREAHGWSLRRTRSRGKK"
     gene            1610..2272
                     /locus_tag="ACHK74_004566"
     CDS             1610..2272
                     /locus_tag="ACHK74_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFP0223269.1"
                     /translation="MSRFRHALRDRDQHILTLRIFSGVLAGILMLVCIGWMTAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAFYIFQQLNAWPKNGEVDYPAKIHALSAYLTP
                     SCREILESDARRRSDAGELLERVRVVYEIPEHGYGGRYNKNPVTVEGRDNWQVQLDLV
                     ADEYFHAEPVKRALVRYPIKVVRMEGDPERNPFGLALDCYSSTPQRLEAASPAAGKGD
                     NQ"
     gene            2272..3180
                     /locus_tag="ACHK74_004567"
     CDS             2272..3180
                     /locus_tag="ACHK74_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFP0223270.1"
                     /translation="MNGAERCLYRSIVMVLSLLVLPLSARADELMKWERIPLPVTLMA
                     GQERVIFADKNVRVGFLPALNGKLRVQSTGGAVYLKAESVFPQTRLQLQDVESGEIIL
                     LDVTAAEKGPTEPVRIVYEGSISTVSQAGTAASARSAEHKKVVPGGDDGSQALRQPVK
                     YAEPLPVLLTRYAAQSLYAPLRTLEAVPGIRPVNTHLPGKITTLYPSEPLTVTPLAAW
                     AAGGRAVVALKLTSTVSRKIILDPRALQGKFIAATFQHCWLGAAGTPEDTTTLYVVTA
                     ERPEQAFIAEPASRRIPSRGEVKHAD"
     gene            3170..4657
                     /locus_tag="ACHK74_004568"
     CDS             3170..4657
                     /locus_tag="ACHK74_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFP0223271.1"
                     /translation="MQIKSNTIVKYVVPCVVVAGILTGLKSCGGDTGEPSSAESSGTL
                     AGLSPDELKALGVEGDTPEDTLRTLIGRMNQVRERQLVLDKQNEKLLKENEMLRKRGT
                     DVSGQVSEAVAGLQKNYEQSQRQFRNEQNALMTQVQSLTEQLKRQQESADAGAGGQES
                     DIPAGLGLDGLGSVTGSAASPGTDGLMWVTPADQVQDEASSVGDKSGPRFPVSFLDDN
                     PLTRQKAAYEQQVKGYSNEHQKASTRAVYTLPENSTLAGSRAMTALLGRVPVNGTVTD
                     PYPFKILVGKDNLTANGIELPDVEGAVVSGTASGDWTLSCVRGQVNSITFVFADGTVR
                     TLPSSDVNGSSNAQAKNNSSTGGIGWISDDDGIPCISGIRKSNASTYLPTIFALSAAG
                     AAGDALSEGQYTSQNNVNGITSTLTGSAGEAALGKALSGGMNETAEWVKQRYGQTFDA
                     IYVPPGQTLAVHITRQLAIDYEDQGRRVRYDFTLPGEVHGTGGLD"
     gene            4693..5085
                     /locus_tag="ACHK74_004569"
     CDS             4693..5085
                     /locus_tag="ACHK74_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFP0223272.1"
                     /translation="MTVMVAVFILSGCSTSKEELLPPGEQSMLEIWQGKGSGGHSRHM
                     TAARDTLRRPLTMPEQQADAHGAISYSRTQESEISQQFPRLPNPDMVMYVFPHLASGT
                     SPVPGYSTVFPFYSRTQYAMPGERTEAL"
     gene            5223..7919
                     /locus_tag="ACHK74_004570"
     CDS             5223..7919
                     /locus_tag="ACHK74_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFP0223273.1"
                     /translation="MTRQDETKVYHACPSVIDFLPWVEYLDEEQCLLLDDGVSVGAVY
                     EVSPAATEGRTAERLEQIRDTVEDALQDSFDEYDSHPWVVQFFCQDENDVDTYVDQLR
                     GYVKPHAEGSSFTEAWLREMERHLKGIARPEGLFRDTLVTGQPWRGQQRRTRMVIYRW
                     IGKNNHDSMPPVAMLNQVCSRVVGALGGAGVRCTRMNGQQVHGWLLRLFNPRPEWVDR
                     DILYRMASRAEPQETPEGMMPVMTDFAESLWFTPPVSDPENGVWWLDGLPHAAVVVEK
                     LRTPPEPGTITGEQARGEKTVNALMDTFPEGTVLCMTIVVQPQDTLEERFTRLSKNAV
                     GENSESLRAREDVATVKKYLGNRHKLYRAGITFLLRAEDMESLKRRRVELTTVLLGAG
                     LQPVRPEFDVGPLNSWLRALPMCFDPDTDKKQWYTRLMWVQHLAGLLPVTGRETGTGH
                     PGFSFFNRGGDVLTFDPLNKLDRTQNAHLLLFGPTGAGKSATLCGSLSQIMAVHRPRL
                     FIAEAGNSFGLLADYFESLGLSVNKISVKPGTGVCLPPFADAHQLVEQGETLQSVDEH
                     SLPDLDEDEGDEEEEKRDILGEMEISARMMITGGDPKEEAALKRADRAMIREALLMAT
                     HTTYREGRQMLPVDLQSALWEISRDTQRNDVRRAKAAEMAESLGMFTQPGSFEAELFN
                     REGKLWPEADVTLIDLGHLAREGYEAQMALTMVSMTNMINNIAERDQYLGRDILFVVD
                     EAHIVTVNPLLSPYMTKVVKMWRKLGAWLWLATQNLKDYPDVSEKMLNMAEWWVCLTM
                     PPEEVNNIARFKSLTEEQKAVLLSASKLSGCYTEGVVLAKRTEALFRVVPPSLYLALG
                     MTEKEEKAERRALMKVHQCSELEAAFHVARKLDGLRGLAGRE"
     gene            7924..8301
                     /locus_tag="ACHK74_004571"
     CDS             7924..8301
                     /locus_tag="ACHK74_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP0223274.1"
                     /translation="MLCLKYPEPEVVSAVHPAGSVFVLPPQGAPGISCTTRDNLERLR
                     GHLAAQLGRVECIRCQPQRVGLNSSVAVMLEGQQGQYVHILLTVSGHESWPSEEEYIH
                     PRWYISVTDAADLFYLLLWLGEG"
     gene            8700..8888
                     /locus_tag="ACHK74_004572"
     CDS             8700..8888
                     /locus_tag="ACHK74_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFP0223275.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDEKMTQAECEKLFAPAKEP
                     GRYIGVRVEDFRCEVVKA"
     gene            9274..9702
                     /locus_tag="ACHK74_004573"
     CDS             9274..9702
                     /locus_tag="ACHK74_004573"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223276.1"
                     /translation="MLKKTILALCISAPFLVAGCDDATKENVSKKAADIKEAAQTQLS
                     DVKDAAEDVKMQAAGKVAEVSEAAQSGLSEVKDAVEEVKTQAAGKVAEVSEAAQSGLS
                     EVKDAVEEVKTQAAGKVAEVSEATQSGLSDVKDTAPEKGG"
     gene            9916..10548
                     /locus_tag="ACHK74_004574"
     CDS             9916..10548
                     /locus_tag="ACHK74_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP0223277.1"
                     /translation="MLLIAGHLRPAFQAHTGRFLIADLSEEVVRRYLRFCTPLSGMAV
                     SPSSLLLQIRPQMSVLAECLIRQLITDEPSPVYFRDAVAFSSLALFEEHPDFHALLTG
                     CIRSCTVRVKNIISADLSRPWMLGEVASLLYISESLLKKKLQEELTTFSELLLKERMQ
                     YAAQLLSAGENRISRVAELCGYASASYFISVFRRYYGITPNQWLPVMQPD"
BASE COUNT         2369 a         2479 c         3424 g         2454 t
ORIGIN      
        1 aaatcattca ggctggaata aatttatttg aacagaatct ggaacagcga gatgcagaaa
       61 ctggctatgc tgacagcccc cagaacatag tttattgttg cccgtattgc ccgtattgcc
      121 cgtattgccc gggttgtttg ccttcatcca caccagtgta ttctcccgta aaaattttct
      181 gttttgcaaa tagagtttat cgctgatcca gtcccctgcc ttacgcatcc tgtacgcact
      241 gtttaactct gacggaggtg cgtatgtttt cttttccggc atcatcctgc cgtgccggcc
      301 gtctggccgg tgccgcgggg atgctgtttc ttttgtcctt tgcattacct gcgcaggctg
      361 ccgaaaagga tgaactggcc actgtaatgc gccagcttga ccacgtgcag gcagcgctgg
      421 agcgggccag aacggcctct ctgcaggctt cttcacaggg ggaacgctac ttttttgatt
      481 atttgcgtgc ccgtgcagat ctgcagacca ttcgtgacgg tattgaccat tatctggccc
      541 cttcccgggc gcaaccccgg tcttccgcag ccgtgaccgg tgactaccgg cgggagcgtc
      601 cctgatggcg atgacggccc tgcagaatac ggccttccgg gctgcttcag gtgaagtgga
      661 tctcagtgtg ctgcatctca tcacgctggg atttctgctg gcagtcgtgt tcctctgggc
      721 agcgtggagt ctgacggatg cctgggcagg ctggcgtagc ggtgatgtcc gggatggtgt
      781 gttcggctgg tttatgttcc gactcgtcct gctgctgatc tgcagtatct ggatgtttgc
      841 gggttgattt cagtgtcttg tttaagtttc aggagaaaat aatgcataaa aaaatgcgca
      901 tctgtcgcca ggcctggtat gccatggcgg tgtttctgtg taccgggatc acacctgctc
      961 tggctgattt gcctcagata gagccgccaa catccggtgg aggtggtgga cttttcggac
     1021 agatcaaagg ctatctccag gatggtgttg tgctgtttgg tcttgtgctc agtgcagttg
     1081 cttttgtgaa ggtggcggaa gcggcgcttt ccaccttctc agaggttcgt gacgggaagg
     1141 ccggatgggg gcagtttggt gccatcgtgg tggttggtgt tgttctgctg gtcgccatta
     1201 tctggctgct gggcaagtcg gcctccatca ttctgtaagg agacatcccc atgcagacca
     1261 ttcgttttct gcccgatcgt ctgaacgtgg agcctgcggt ttttcgtggt tttaccacac
     1321 cggaactggg gctggcggcc ctgacaggcg ctgtggcagg ggtgatctgg ccgttaccgc
     1381 tgattccgct acttggctgg gtgattatcc cgacagggat gttgctgact cccctgctgc
     1441 tggtctggtt cggtggcgtc tggatcagcc ggctgaagcg gggaaaacct gacaactggc
     1501 tctggcagca tctggcgaag aaaagcagtg catggggtgg tggccggaaa acatttatcc
     1561 gggaggcgca tggctggtcc ctgcgccgta cccgtagcag ggggaaaaaa tgagtcgttt
     1621 tcgtcatgct ctgcgggatc gcgaccagca tatcctgaca ctgcgtattt tcagcggtgt
     1681 gctggccgga attctgatgc tggtctgtat cgggtggatg acggcaccgg acagactgac
     1741 aattcacaat ccgccggatc tgcgttccgg cagtacccgt ccctggtggg aagtcccgcc
     1801 gtcaacggtt tattcctttg ccttttatat ctttcagcaa ctgaatgcct ggccgaagaa
     1861 cggagaggtg gactacccgg cgaaaattca tgcgctctcc gcctacctga cgccatcctg
     1921 tcgggagatt ctggagagtg atgcccgccg tcgcagtgat gcgggggagc tgctggagcg
     1981 tgtgcgcgtg gtgtatgaaa ttccggaaca tggctacggg ggcaggtaca acaaaaatcc
     2041 ggtgacggtg gaagggcggg ataactggca ggtacagctg gatctggtgg cagatgagta
     2101 ttttcatgct gagccggtaa aacgggcact tgtccgttat ccgataaagg tggtgcgcat
     2161 ggagggggat cctgaacgta atccgttcgg actggcgctg gactgctaca gcagtacacc
     2221 acagcggctg gaagcagcct ccccggcagc cgggaagggg gataatcagt gatgaacgga
     2281 gctgaacgtt gtttataccg tagtattgtg atggtcctgt cactgctggt tttaccactg
     2341 agcgcacggg ctgatgagct gatgaaatgg gagcgcattc ccttacccgt taccctgatg
     2401 gcaggacagg agcgggttat ttttgcagat aaaaatgtgc gggtgggctt cctgccagca
     2461 ctgaacggaa agttgagggt acagagtacc ggtggggcag tctatctgaa agcagagagt
     2521 gtttttccgc agacgcgtct gcaactgcag gatgtggaga gcggagaaat tattctgctg
     2581 gatgtgacag cagcagaaaa agggcccaca gagcccgtac ggattgtgta tgagggaagc
     2641 atcagtacgg tcagtcaggc tggcacagcg gcgtctgcac gctctgcaga acacaaaaag
     2701 gtggtacccg gcggggatga cggctcgcag gccctccgtc agccggtgaa gtatgcagag
     2761 cctttacccg ttctgttgac ccgctatgcg gcacagtcgc tgtacgcacc gttacggacg
     2821 ctggaagcgg taccgggtat ccggccggtt aatacacatc tgcccgggaa aatcaccaca
     2881 ctctatccgt cagagccact gacagtcacg ccactggcag cctgggctgc gggtgggcgt
     2941 gcagttgttg cactgaaact gacgagtacg gtaagccgca aaattatcct tgatccgcgg
     3001 gcacttcagg gaaaattcat tgccgcgact ttccagcact gctggctggg tgcagcaggt
     3061 acccctgagg ataccaccac gctctatgtg gtgacggcag aacggccgga acaggcgttt
     3121 atcgcggagc ctgcctcccg tcgcattcct tcacgggggg aggtgaagca tgcagattaa
     3181 atccaatacg atcgtgaagt atgtggtgcc ctgtgtggtg gtggcgggga tcctgacggg
     3241 attaaaaagc tgtggaggag ataccgggga gccctcgtct gcggaaagct caggaacgct
     3301 ggccggactg tcaccggatg aactgaaggc cctgggcgtg gaaggagaca cacctgaaga
     3361 taccctgcgc acactgattg ggcgtatgaa tcaggtgagg gagcggcaac tggtactgga
     3421 caaacagaat gaaaagttgc tgaaagaaaa tgaaatgctg cgcaagcggg gcacggatgt
     3481 ttccggtcag gtcagtgagg cagtggcagg attgcagaaa aattatgagc agtcgcagcg
     3541 ccagttccgg aatgaacaga acgcgttaat gacacaggtt cagtcactga cagagcaact
     3601 gaagcgtcag caggagagtg ctgacgccgg cgccggagga caggagagcg atattccagc
     3661 aggtctgggc cttgacggac tggggagcgt gacaggatcg gcagcctcac cgggaacgga
     3721 tggtctgatg tgggtgacac cggccgatca ggtgcaggat gaagcgtcgt cggtggggga
     3781 taagagcgga ccgcgttttc cagtctcttt tctggatgac aatccactga cccgtcagaa
     3841 agcggcttat gaacagcagg tgaagggata cagcaatgaa catcagaagg cgagcacccg
     3901 ggcagtgtat accctgccgg agaactcgac gctggcaggc agccgggcga tgacagcgct
     3961 tctgggacgg gttccggtga acggaaccgt gacggatccc tatccgttca agatcctggt
     4021 gggtaaggat aacctgacgg caaacggaat agagctgccg gatgtggaag gtgcagtggt
     4081 gtccggtact gcgagtggtg actggacgct ctcctgtgtc cgtgggcagg tgaacagcat
     4141 cacgtttgtt tttgccgacg ggacagtgcg tacgctgccg tcatcagacg tgaacggcag
     4201 cagtaacgca caggcaaaaa ataacagcag tacaggaggc attggctgga tttcggatga
     4261 tgacgggatc ccgtgtatca gcggaatccg taaatccaat gcctccacgt atctgccgac
     4321 aatatttgcc ctgtctgcag ccggcgctgc gggtgatgca ctgagtgagg ggcagtacac
     4381 ctcacagaat aacgtcaatg gcatcacatc aacactgacc ggcagtgccg gagaggcggc
     4441 acttggaaaa gccctgtccg gcgggatgaa tgaaaccgcg gaatgggtga aacagcgata
     4501 cgggcagacg tttgatgcca tttatgttcc cccggggcag acgctggcgg tgcatatcac
     4561 gcgtcaactg gccattgatt atgaagatca ggggcgccgg gtacgttacg acttcactct
     4621 gccgggggaa gtccacggta ccggtggtct ggactgatgt aaggagatcc cgtgaaacga
     4681 cagttatctg taatgaccgt catggtggcg gtatttattc tgtccggctg ctcgacatcg
     4741 aaagaggagc ttctgcctcc gggtgaacag agtatgctgg agatctggca ggggaaagga
     4801 agcggaggac attcgcggca catgacagcg gccagggaca ccctgcggcg tccgctgaca
     4861 atgccggagc agcaggcaga tgcgcacgga gccataagct acagccggac acaggaaagt
     4921 gagatcagtc agcagtttcc gcggctgccc aatcctgaca tggtgatgta tgtttttccg
     4981 catctggctt ccggtacgtc accggttccg ggatacagca cagtatttcc tttttacagc
     5041 cggacgcagt atgccatgcc gggtgaacga acggaggcgt tgtgatgcga ttttccctgt
     5101 ttacagacac aaaaaaacgg gtttcttccc cggtgtcgca gcatgacgga gaagcggtat
     5161 ctccggatat gaaagaaaga gtggcccctc aggtacgaaa gccactgtca aggccgggga
     5221 agatgacccg acaggatgag acgaaggttt accatgcctg tccgtcggtg attgattttc
     5281 tgccatgggt ggaatatctg gacgaagagc agtgcctgct gctggatgat ggggtgtcgg
     5341 ttggtgccgt gtatgaggtg agtccggccg ctacggaagg gcggaccgca gagcgtctgg
     5401 agcaaatccg ggatacggtg gaggatgcgc tgcaggacag ttttgatgaa tatgactcac
     5461 atccctgggt ggtgcagttc ttctgtcagg atgaaaacga cgtggacacg tatgttgacc
     5521 agctcagggg gtacgtgaaa cctcatgcag aaggctcctc atttacggag gcctggctga
     5581 gggagatgga gcgccatctg aaggggattg cccgaccgga aggtctgttc agggatacgc
     5641 tggtcactgg tcagccatgg cgtggtcagc agcggcgcac gaggatggtg atttaccggt
     5701 ggatcgggaa aaacaaccac gattcgatgc cgccggtggc aatgctgaac caggtctgtt
     5761 cgcgggtcgt gggggcgctg ggtggcgcag gcgtccgctg tacacggatg aacgggcagc
     5821 aggtgcatgg ctggctgctg aggctgttca atccccggcc ggaatgggtg gacagggata
     5881 ttctgtaccg tatggcatcc cgtgcagaac cgcaggagac accggaggga atgatgccgg
     5941 tgatgaccga ttttgcagaa agcctctggt tcaccccacc ggtatctgat ccggaaaacg
     6001 gcgtgtggtg gctggacggg cttccgcatg ccgctgttgt ggtggagaaa ctgcgcacac
     6061 caccggagcc gggaaccatc acaggggaac aggcccgggg cgaaaagaca gtgaatgccc
     6121 tgatggacac ctttccggag ggaacggtgc tgtgtatgac aattgtggtc cagccacagg
     6181 acacactgga agagcgcttc acgaggttgt cgaaaaatgc ggtcggggag aacagtgaat
     6241 ccctgcgtgc ccgggaggat gtggcgaccg tgaagaaata tctgggaaac cggcacaagc
     6301 tctaccgtgc cgggatcacg tttctgctgc gggcagagga tatggagagt ctgaagcgca
     6361 gacgggttga actgacgacg gtgctgctgg gcgcgggctt acagccagtg cgcccggagt
     6421 ttgatgtcgg accgctgaac agctggctgc gcgcgctgcc catgtgtttt gatcctgata
     6481 cggataaaaa acagtggtac acgcgtctga tgtgggtaca gcatctggca gggctcctgc
     6541 cggtgacggg aagggaaacc ggtacggggc atcccggatt cagtttcttt aaccgtggcg
     6601 gggatgtgct gacctttgat ccgctgaaca agctggaccg gacccagaat gcacatctgc
     6661 tgcttttcgg gcctaccgga gccggtaaat cggccacgct gtgtggttcg ttgtcgcaga
     6721 taatggcagt tcatcgtccc cgtctgttta tcgctgaggc cgggaactcg tttggtctgc
     6781 tggcggatta ttttgagagt ctcgggcttt cggttaataa aatcagtgtc aaaccgggaa
     6841 cgggggtgtg tcttcctcca tttgcggatg ctcatcaact ggtggagcag ggagagacgc
     6901 tgcagagtgt ggatgagcat tcactgcccg atcttgatga agatgagggg gatgaagagg
     6961 aagagaaacg cgacattctg ggggagatgg aaatctctgc ccgcatgatg atcaccggag
     7021 gggatccgaa agaagaagct gcgctcaaac gtgcggacag ggcgatgatc cgtgaggcac
     7081 tgctgatggc cacgcacacc acgtaccggg aggggcgtca gatgctgccg gtggatctgc
     7141 agagtgcgtt gtgggaaatt tcgcgggaca ctcagcgtaa tgatgttcgc cgggctaaag
     7201 ctgctgagat ggcagagtcg ctggggatgt tcacccagcc gggcagtttt gaagcggagc
     7261 tgtttaaccg ggaaggaaaa ctgtggccgg aagcggatgt gaccctgatt gatctggggc
     7321 atcttgcccg tgaaggttat gaagcccaga tggcactgac catggtgtca atgaccaata
     7381 tgattaacaa cattgccgag cgcgatcagt atctgggacg ggatattctg tttgtggtgg
     7441 atgaggcgca tatcgtgacg gtaaacccgc ttttgtcccc gtatatgacc aaagtggtga
     7501 aaatgtggcg caagctgggg gcctggctgt ggctggccac gcagaacctg aaggattatc
     7561 cggatgtctc ggagaagatg ctgaatatgg cagagtggtg ggtgtgcctg accatgccgc
     7621 cggaggaagt gaacaatatt gcccgtttta aatccctgac ggaggagcag aaggcggtac
     7681 tgctgtcagc cagtaaactg tccggctgct atacggaggg ggtggtactg gcgaagcgca
     7741 cagaagcgtt gttccgggtt gtgccaccga gtctgtatct ggctctgggg atgacggaga
     7801 aagaagagaa ggctgaacgc cgcgccctga tgaaggtgca ccagtgcagc gaactggaag
     7861 cagcgtttca tgtggcacga aagctggacg ggctacgtgg tctggctggc agggagtgag
     7921 actatgctgt gtctgaagta tccggaaccg gaagtggtgt ctgctgttca ccccgccgga
     7981 agtgtgtttg ttctgccgcc acagggtgcg ccggggataa gttgcaccac acgggataat
     8041 cttgaacgtc ttcgcgggca tctggcggcg caactggggc gggtggagtg tatccgttgc
     8101 cagccacaga gggtgggtct gaacagcagt gtggcggtga tgctggaagg tcagcagggt
     8161 cagtatgtgc atattctgct tactgtgagt gggcatgaga gctggccgtc ggaggaggag
     8221 tatattcatc cccgctggta tatatcagtg actgatgcgg cggatttgtt ctatctgctg
     8281 ttgtggctgg gagaggggtg atatgaggag cgatatcgat tttccgggac cactgtatcc
     8341 atcgctgtca gatcaaattg ccagtgaacc tgtgctgttt ctggcgggag ctgtgggtgt
     8401 ggctgtgttt tcatgggggc ttgcgaattt ttcatggatg cttgcgaatg tactggcccg
     8461 gatgctgtca gggttgtact cccgtgtcag gaggagggta aagaactctg aaaaatagcg
     8521 ggagagaggt gctgccctga tgccttcgtt gtattctgcg acgctgttgg tgcccgtatt
     8581 ttgtgcgggt accgtacaga atcaccgcat gagccattgc tgagacgctc actttttttc
     8641 gcatgcatta ccggagtgat ggctcagccg gtgattttgt tgttgagatg agagccagta
     8701 tgggaaagcg atataaaata actgcgacaa taatcaaacc aggcaatatg ccggttcagt
     8761 ggctgcgtta ccaggatgaa aaaatgactc aggctgaatg tgaaaaactg tttgcgcccg
     8821 caaaagagcc gggacggtat attggcgtca gggtggagga cttccggtgt gaagtggtga
     8881 aggcctgagt atgaaaataa aaaacctccg gcggagtcgg agggggcaaa aacagatagt
     8941 cccggagtgt atctgtgtaa atggtgttat tggtataata atggtggtaa aaaccacggg
     9001 agaatttatt cattttattc gtcatgaaat caacggtaat agtgtgtttc tgtgacggga
     9061 ataaaaaaat acagcacagc cgatgacccg gtgctgaaaa caggttaaat gtagtgggca
     9121 catttccggt aatatcagga acagcatgtt atggttataa aagcatatac cgggagtgtg
     9181 ccctgccact gtgatgttct ggttgtcttt tgaccatttt ccggggacga aatataagct
     9241 gttctgcgtt aacacttaat aatgagagat tgtatgctga aaaaaacaat tctggcgtta
     9301 tgtatttccg caccatttct ggttgcgggc tgtgatgatg cgacaaagga gaatgtaagt
     9361 aagaaggcgg cagatataaa ggaagctgcg cagactcaac tcagtgatgt gaaggatgcc
     9421 gcggaggacg tgaaaatgca ggctgccgga aaggtggcag aggtttctga agcggctcag
     9481 tccgggctca gcgaggtgaa ggatgccgtg gaggaagtga aaacacaggc agccggaaag
     9541 gtggcagagg tctctgaagc ggctcagtcc gggctcagcg aggtgaagga tgccgtggag
     9601 gaagtaaaaa cacaggcagc cggaaaggtg gcagaggtct ctgaagcgac tcagtccggt
     9661 ctcagtgatg tgaaggatac cgctcctgaa aaggggggct gaaatatccg taacggagga
     9721 gtaaaagagc gccgtcggtg cagttttttt cagcacccgg tgacacggaa ttttatatac
     9781 ggggcatgtc tgtctcttta ataaatcaac atatttaccg gatatccgat gccctgtacc
     9841 tgcgctgtcc ttatggctct caaacagaca atgctttgtc tggagcacag gaactctgtc
     9901 atacctgccg gcacaatgct tctgattgcc gggcatctgc gccctgcttt tcaggctcat
     9961 accggacgtt tccttatcgc tgacctcagt gaggaggtgg tgaggcggta tctgcggttc
    10021 tgcacgccat tatccggtat ggcagtcagc ccgtcgtctt tgcttttaca gatccggcca
    10081 cagatgtccg tgctggcgga atgtctgatc agacaactta ttacagatga accatcgccg
    10141 gtgtacttca gggatgcggt ggctttttcc agtctggcgc tgtttgagga acatccggat
    10201 ttccatgccc tgctcacggg gtgtatccgg agctgcactg tccgggtgaa gaatattatc
    10261 agtgctgatt tgtcccgtcc atggatgctg ggtgaagtgg ccagtctgtt atatataagt
    10321 gagagtttac tgaagaaaaa gcttcaggaa gagctgacga ccttcagtga gcttcttctg
    10381 aaagagagaa tgcagtatgc cgctcagctt ttgtctgcgg gagaaaaccg tatttcccgg
    10441 gtggcagagt tgtgtggcta tgccagtgct tcttatttta tttctgtgtt caggcgttat
    10501 tatggtatta ccccgaatca gtggcttcct gtgatgcaac cggattaatt tgattcagat
    10561 gcagagtgtg cgttattcag aggaagaggc ttaattttta tcatccggtt ttgaatttat
    10621 tgctgattgt ccctttgcct ttgcgcacgc tgtcagatac cagttgtctg acggagtcgc
    10681 atcatgtacc gactgttact gtattccttc ctgatactac cggtgg
//
LOCUS       DAZGSP010000146        10693 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZGSP010000146 DAZGSP010000000
VERSION     DAZGSP010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.147"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1201
                     /locus_tag="ACHK74_004575"
     CDS             <1..1201
                     /locus_tag="ACHK74_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFP0223278.1"
                     /translation="GTGQAQACLMNIIGTASQDGVLHIYIGGRRYLLAVTKGQKGKEL
                     IDALVSVINADADAPFSAYAGQVADNASGLKGSMGINARFTGECSVHDIRVNYHDGET
                     TPGGLTIELGYPKTKATNPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWG
                     PAKMSDGVLWMAHTGTFGEVQAFGAKRNDFLATCTFIPKAPEPDYLWAAAVCATCAPS
                     LGADPARPLQTLALPSRMAPAPADRLTREERNSLLWGGIATVSVAAGDVVQIERQVTM
                     YRQNVYGESDPSYLDVETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTP
                     EIMKLQLISLGEEWVELGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFLA
                     AQIRFIL"
     gene            1210..1575
                     /locus_tag="ACHK74_004576"
     CDS             1210..1575
                     /locus_tag="ACHK74_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFP0223279.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISVKFASATSTRVQ"
     gene            1590..2066
                     /locus_tag="ACHK74_004577"
     CDS             1590..2066
                     /locus_tag="ACHK74_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFP0223280.1"
                     /translation="MTTRKKKTAVSEAAVMGAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAVSGSD"
     gene            2193..4268
                     /locus_tag="ACHK74_004578"
     CDS             2193..4268
                     /locus_tag="ACHK74_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFP0223281.1"
                     /translation="MARNLRASLIVDLLGNISAKSRQWSQELGAFSRSGQAGLGGLGN
                     AARRAGQETDVLGSRMQRTLAGVRGSIRHVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNRGFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWGLAGVMQ
                     EYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQMYARGSIQAADAN
                     ILATYGINAYRVLADRLGVDQKVVRKLGEKGLLGPDSIRLLFQTLAEQARGAQKDAMN
                     SWSGLTAMMGDVWDQFARDVMDSGPFEKLKGNLRGFLSWVDSAKSDGSYHSLAESTAS
                     AMNQGFEYARDAVTGFYQAIRKVRDTLQALRDAGYGDALDRIGQGAQTAAKYLLYMYL
                     ATRALKMARAVGTGIIRPGAALLGYGLSAAAFLTSPFRRSPPAGTPPGTSQGRGQRFI
                     NFLTGVNPAAVQPVLVTNWPTGGLSGTGATDTSSGGRQGRGRRKRGPGRGRPVTPPLP
                     PSPVPPSPSSGGAGFWGRMMGRAGGLLSSVGSRMGLGRFSGFFRSAGGLAGRLGGGAL
                     WAGAMAAPVLLDGSASATDKGEAVGSLAGSIAGGALGAAAGPVGIAIGSTVGSYLGNY
                     LGGWLTEAWQKLRGSDDESSGQAVQKASARVELVAPEGWQARSIDIDDTSGHGLDVNV
                     WNGGNYGLW"
     gene            4255..5604
                     /locus_tag="ACHK74_004579"
     CDS             4255..5604
                     /locus_tag="ACHK74_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFP0223282.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRREYPLRETGGADD
                     LGPKLAEFTFSVLVMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRYN
                     AAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGQVFETLEAGWTVISDGM
                     HDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASSF
                     AGLFSALITLPSLPSLSSGGLNTRPGGSIGRTTSAVSQGMPQLYRTLSSLRYVLDEQD
                     NPQTLIGLTPAAQKNIRLIRAVMQSAAVVAQAQTVGKLLDQVLSQETLPDSDAAHRTW
                     PVWLESSVDLQRINRDLSEALERQVMTLSGQGYTATALTLRDARLALTEDLNTRGVQL
                     PGATVVTVRTTEPALVTLYRATGNSTGWQRFVRRNGIVDPLFIPGGHSVEVISEQQG"
     gene            5588..6712
                     /locus_tag="ACHK74_004580"
     CDS             5588..6712
                     /locus_tag="ACHK74_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFP0223283.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRTLEHIASDLCAPFGVTVRWQVNDATAARPFSTFTLENSETVADALTRAARHR
                     GVLVTSNAAGELVFTQAGSQRGDTLTLGENLLDLDHNVDHRLRHSEYRVRGHGRGGGH
                     AGDALTAGTLAAPVGTVTDSAIHRYRPKIVLADHAVDADGARQRAVREMRRAVARSVR
                     LTATVRHWFRENGQLWDINLLTAVTAPCTGVEERDLLVCQVEFSLDANHGETTRLILA
                     PRDGFIVPAEPGNSGSGNAGDVDAFVRAQMKKQGIKFNDE"
     gene            6702..7316
                     /locus_tag="ACHK74_004581"
     CDS             6702..7316
                     /locus_tag="ACHK74_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFP0223284.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTMDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEAHFTGNVTVDKNLHVKGNV
                     SIDGTGRSQGTFTMSEAVIAGITYSGHVHHDNGEGSKTGGPENG"
     gene            7309..7746
                     /locus_tag="ACHK74_004582"
     CDS             7309..7746
                     /locus_tag="ACHK74_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFP0223285.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPVGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GAATSVVCHAMRVGHARLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            7746..8828
                     /locus_tag="ACHK74_004583"
     CDS             7746..8828
                     /locus_tag="ACHK74_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFP0223286.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FEVRSPLKSVMAGDTELLTVGEITWL"
     gene            8819..9379
                     /locus_tag="ACHK74_004584"
     CDS             8819..9379
                     /locus_tag="ACHK74_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFP0223287.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEETI"
     gene            9379..10290
                     /locus_tag="ACHK74_004585"
     CDS             9379..10290
                     /locus_tag="ACHK74_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0223288.1"
                     /translation="MFHVDNNSGVANMPALAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARSYLGLGALATKNSLGPGDVNALAKDQNLADLENAGTARNNLDVYSKSEGDNRYLRR
                     EQNGADIPDKGAFIDNVGLRETVNKAADALPSGGTAVAANRLATPRNINGVPFDGTQD
                     INITSGMTQSTADGRYVQNVQLGAQSYHSPGGNEISWNYSAPSGCMLSGINVQETGSR
                     SADNIGGVYYRPVQIYINNAWRTVSSV"
     gene            complement(10325..>10693)
                     /locus_tag="ACHK74_004586"
     CDS             complement(10325..>10693)
                     /locus_tag="ACHK74_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0223289.1"
                     /translation="IICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISK
                     VPVNWKTVAEKRRSSLLQEANETVDDWKTELKLDMISDENKLQLTRWMAYIRQLKEMH
                     FNDIASEGHYQAIPWPEKPE"
BASE COUNT         2374 a         2723 c         3252 g         2344 t
ORIGIN      
        1 cggcaccggt caggctcagg catgtctcat gaatataatt ggcaccgcct ctcaggatgg
       61 tgttctgcac atttatatcg gcggtcggcg ttatctgctg gctgtcacga aagggcagaa
      121 gggtaaggag cttattgacg ctctggtctc tgttattaat gccgatgctg atgcgccctt
      181 cagtgcttat gcaggtcagg tagcagataa cgccagcgga ctcaagggaa gtatgggaat
      241 taatgcccgt tttaccggtg aatgttccgt acatgatatc cgtgttaatt atcatgacgg
      301 agaaaccaca ccgggtggac tgaccattga acttgggtat cccaaaacaa aagccactaa
      361 cccggatatc acccgaagcg tggcgggtat gggcgatcgc cagtacaact atgtcgtcat
      421 gccctataag gatgatgcaa acctgaaaat catcagtgat gagcttctga aacgctgggg
      481 accggccaaa atgtctgacg gtgtgctgtg gatggcgcat accggcacgt ttggtgaggt
      541 gcaggcattc ggggcaaaac gcaatgattt tctggcgacc tgcacattca ttcccaaagc
      601 ccctgaaccg gattacctct gggcagcggc ggtctgcgcc acctgtgcgc cgtctctggg
      661 ggcagatcct gcgcgtccgc ttcagacgct ggcgcttccg tcacgcatgg ccccggcccc
      721 ggccgatcgt ctgacgcgtg aagagcgtaa cagtctgctg tggggaggga ttgccacggt
      781 cagtgtggct gccggtgatg tggttcagat cgaacgtcag gtgaccatgt atcgccagaa
      841 cgtgtacggc gaaagtgatc ccagctatct ggatgtggaa accatctaca ccctctcctg
      901 gctgcgttat tccctgcgta cttttatcac acaacgtttt ccgcgccata aactggctga
      961 tgacgggacg cccgttcgtg ccggtcagaa tatcgtgacg ccggagatca tgaagcttca
     1021 gttaatttcg ctgggtgaag agtgggtgga actcgggctg gtcgaaaatc tggacacctt
     1081 taaaaagaat ctgcttgtgg agcgtaatgc gtcagatcga aaccgtcttg acgtgttatg
     1141 cacgccggat ctggttaacc agttccgctt cctggcagca cagatccgtt tcattttgtg
     1201 aggtaaggca tgagcggaaa acagtatcag ggcacggcca ctatccgtgt gaacggtcag
     1261 gaatacgaga cgcttgaggg ggctacattt tctccgtcag gttttgaacg tgaagtggtg
     1321 aaaggcgcga aagtctatgg ttaccgccag aaaccacgtg aagcgacgct ggactgcaaa
     1381 tttccggcag gcggagaagg ttcaccagcc gccgatgaaa tcaacaactg gactgctgtc
     1441 actatcgagt ttgtggcgga taccggcgaa gtccacatga tgacgaaggc ctggagcagt
     1501 gaaccggcct cgcttgacgg tggcggggag atctccgtga agttcgccag cgccaccagt
     1561 acccgtgttc agtgatcagg aaaaataaaa tgaccacacg taagaaaaaa acggcagttt
     1621 ccgaggcagc cgtgatgggg gcaatccggg aagcacttga gggggctgat ccacgcactg
     1681 ccgggctgac agagcaactg gcaaagggat atgtggatct gcttgatggt ctgccgtttg
     1741 gtgagacccg tgaatatcgt gtcacgttcc gggaactgac agcgaaagac agtattgacg
     1801 cagaagcaga agccgagcgc gtggtggaga caaacaatgg cccgatgctg atagcgtctc
     1861 cgtcgttacg cggtgttgca ctgctgcgcc gtcagattgc cgcagtggga gacattgaag
     1921 gtccgttgtc accccgtcag attggacagt taagcgagcg cgatctctcc cgcctgatgg
     1981 cagcggtcag cctgctggat accgcgcttg ccggaaagct ggcagctgat cgggggcgat
     2041 caggcgcagt gtcgggatca gattgaagaa gcggcaatta tcctggggat ggtgacaaaa
     2101 agtggcccgg aatgggcgct taacctcccc ctgtcgcaac tttaccggca ctgccgacag
     2161 acagaaaaaa tcatcagaac gaagcagtaa gtatggcccg aaatctcaga gcatctctga
     2221 tagttgattt gctcggcaat atctcagcca aatcccgcca gtggtctcag gagctggggg
     2281 cattctcacg ctccggccag gcggggctgg gtggtctggg aaatgcggcc cgccgtgccg
     2341 ggcaggaaac agatgtactc ggaagcagga tgcagcgcac gctggccggg gtgcggggca
     2401 gtatccgcca tgtcacttct gattttgacc gtcttcaggg cagtattacc ggcaccatcg
     2461 ggcggatcag caatctttac ggaatgctgg ccggtggtgc tgctgtatat ggctttaacc
     2521 ggggatttat tcgcccggca gcggagatgg aaacctatat gattcgcctg aactcccttt
     2581 acaaagggga tcgggcgaaa actgatgatg tccgcaggtg ggcaatacag aatgcgaagg
     2641 aaaccacctg ggggctggct ggcgtcatgc aggaatatac gtccagtctg ggctttggta
     2701 tgagtgacag ggaggcccga aattttgtca ccatgcttca ggatcagggt gccgtgggcg
     2761 gctggtcact gtccgatgca cagggggcat ccctgcaact gaaacagatg tatgcaagag
     2821 gcagtattca ggcagcggat gcaaatattc ttgccaccta cggcattaat gcctaccgcg
     2881 tgctggctga tcgtctgggt gtggaccaga aagtggtcag aaaacttggt gaaaaagggc
     2941 tgcttgggcc ggacagtatt cgcctgttat ttcagacgct ggcggaacag gccaggggag
     3001 cacaaaagga cgcaatgaat tcctggagtg gcctgacggc catgatgggt gacgtctggg
     3061 atcagtttgc ccgggatgtg atggacagtg ggccatttga aaaactgaag ggtaatctca
     3121 ggggatttct ttcatgggtt gacagtgcca aatctgatgg cagttaccat tccctggcgg
     3181 aaagcacggc atcagccatg aaccagggct ttgagtatgc ccgtgatgca gtgacagggt
     3241 tttatcaggc gatcagaaag gtacgcgaca cgcttcaggc tctgcgggat gcgggctatg
     3301 gtgatgcgct ggatcgcatt ggtcaggggg cacagaccgc cgcgaaatac ctgctgtaca
     3361 tgtatctcgc cacccgtgcc ctgaaaatgg cgcgggctgt cggaacgggg attatacgtc
     3421 cgggtgctgc attgctggga tacggattat cagcggcggc cttcctgacc tcacctttcc
     3481 ggcgctctcc tccggccgga acaccaccag gcacatctca gggacgaggg caacgtttta
     3541 taaactttct taccggggta aatccggcag ccgttcagcc agtacttgta acaaactggc
     3601 ccacaggagg actgtccggc accggcgcga ccgatacatc ctcaggcgga cgtcaggggc
     3661 ggggccgcag gaaacgcgga ccggggcgcg gaagaccggt cacgcctccc ctgccaccgt
     3721 ccccagtgcc accgtctccc tcttctggtg gggcagggtt ctgggggcgc atgatggggc
     3781 gcgccggtgg gttactttcg tctgtcggta gccgcatggg gctggggcgt ttctccggct
     3841 ttttccgcag tgccggtgga ctggccggcc ggctgggtgg aggtgccctg tgggctggcg
     3901 cgatggcagc cccggttctg ctggatggca gcgccagtgc cacagacaag ggagaagcgg
     3961 tcggctctct tgcaggcagt attgccggtg gtgcgctggg ggcggcagcc ggacctgtgg
     4021 ggattgccat tggttctacg gtgggcagct atctcggtaa ctatctgggg ggatggctga
     4081 ctgaagcctg gcagaaatta cggggcagtg atgatgaaag cagtggacag gcggtacaaa
     4141 aagcctctgc ccgtgtggag ctggttgccc cggaagggtg gcaggctcgc agtattgata
     4201 ttgatgacac gtccggtcac ggactggatg tgaatgtctg gaacggaggg aattatggcc
     4261 tctggtgacg gtcgtggtgc gttccgtggt gtgccgttcc tcgtctggcg tgagcagcgc
     4321 gaacgtggtg ggcgaaacat tgtccgtcgg gaatatccgt tacgggaaac cggtggtgca
     4381 gacgatctgg gacctaaact ggctgaattt acgttcagtg tacttgtgat gggggatgat
     4441 gttcagacac agcgaaatcg tctgcgtgat gcccttcgtg ctcccggtgc cggggagctg
     4501 cttcacccgg attacggcac gttaaatgtg ctgataaaca gctttgaaag tcgttataac
     4561 gccgctgaac agggcgtggt tgagttcacc attaatgtca cgccggtaag tgatgatacc
     4621 gcacctgctg tcacacagga cacggccgcc attctggagc agaagagtac caccgcgctc
     4681 gggcaggttt ttgaaaccct ggaagcgggc tggacggtga tctctgacgg aatgcacgat
     4741 gtccaggcaa tgaccgaaac catcagtgat aaggtgtccg ccctggaaaa tgccgtttcc
     4801 ggcatgggga ttgtacagga tatcagtgcc tttactgcca cgttcacggc gctgaaaggg
     4861 aatgccacgg cattactgac agcaccttcc cgcatggcct catcttttgc ggggcttttc
     4921 agtgccctga tcaccctgcc atcgctgccg tcactgtctt caggaggcct gaatacccgg
     4981 cccggtggca gtatcggccg gacgacatcc gccgtctctc agggaatgcc gcaactgtac
     5041 aggacattat cttccctgcg ttacgtgctg gatgagcaag ataatccgca gactctcatc
     5101 ggtctgacac cggcagcaca aaagaatatt cgtctgatac gggcggtaat gcagagtgct
     5161 gccgtggtgg cccaggcaca gaccgtggga aaactgctgg atcaggttct cagtcaggaa
     5221 accctgccgg acagtgacgc agcccaccgt acctggcctg tctggctgga aagttcagtt
     5281 gatcttcagc gcattaaccg tgacttaagc gaagcgctcg aacggcaggt gatgacactg
     5341 tccgggcagg ggtataccgc cacggcgctg acgcttcgtg atgccagact ggcactgaca
     5401 gaggatctga atacacgggg agttcagcta cccggtgcaa cagtagtgac tgtacgtacc
     5461 accgagcctg cactggtgac cctgtaccgt gccaccggga acagtaccgg ctggcaacgt
     5521 tttgtgcgcc gtaacggtat tgttgatccg ctgttcattc ccggaggcca ttcagtggag
     5581 gtgattagtg agcagcaggg ttgaactgta tctgggcggt gagatttttt ccggctggct
     5641 gacggtgagt gttcgtcgct ctcttgaaca tctggcgggc tcctttgaac tgggggtaat
     5701 gatgcccggt gtacgccttc cgtcatccgt ccgtgccggt cagtctctgg aattgcgcat
     5761 tgacggtcag cctgtgatca ctggctggct ggatcaggtc cggcagcgca tcagcgccac
     5821 gcgttttcag atcacgctca gcggacggga taaaaccggt gacctggtgg actgttcagc
     5881 cattcatccg ggcagccagt ggaggaaccg cacgctggag cacattgctt cagatttgtg
     5941 tgctccgttc ggggtcacgg tgcgctggca ggtaaatgat gcaacggcag cccggccctt
     6001 ttccaccttc acactggaaa actcagaaac cgtggcagat gcgctgacac gggccgcgag
     6061 acacagaggc gtcctggtaa ccagtaatgc cgccggtgaa ctggttttca ctcaggccgg
     6121 cagtcagcgt ggcgacacgc tgacgctggg cgaaaatctg ctggatttgg atcacaacgt
     6181 ggatcaccgt ctgcgccaca gtgaataccg tgtacggggg cacgggcgtg gtggtggtca
     6241 tgccggggat gcactgacag ccggaacgct ggccgcaccc gttggtacgg tgacagacag
     6301 tgccatccac cgttacagac cgaaaattgt gctggcggat catgctgttg atgcagacgg
     6361 tgcacgccag agggctgtcc gggaaatgcg ccgggcggtt gcccgctctg tgcgcctgac
     6421 agccaccgtg cggcactggt ttcgggagaa cggccagttg tgggatatca acctgctgac
     6481 ggctgtcacg gctccctgca ccggagtgga agagcgtgat cttcttgtct gtcaggtgga
     6541 gttttcgctg gatgcaaatc acggcgaaac cacccgtctg attctggcac cccgtgacgg
     6601 ctttattgtt ccggcagagc cgggaaacag cggaagcgga aatgcgggtg acgtggacgc
     6661 cttcgtgcgg gcacagatga aaaaacaggg gattaaattc aatgatgaat gacgaagtca
     6721 tcagccgcct tctggccccc gtaatgcggg gtgttcgtct gctgttcggg cgtggtgtac
     6781 tgaccggcac aatggacacg ctgaaaatcc agaatgtgca aatcaccggt atggatggtg
     6841 aaacctttga tgacgttgaa cgcccccagc agtacgggca aatcagcgtc cccctgcctg
     6901 gtgcggaagt ttttctggcc tgtgctggcg gacaacggga tcaggccgtg gtgcttgtgg
     6961 tggaagaccg ccgcagtcgc ccgaccggac ttaccgccgg agataccggc gtgtatcacc
     7021 atgaggggca ccgtatacgt ctgacaaaga acggccggat cattgtgacc tgtaagacgc
     7081 tggagattta cgccgatgaa ggtgttcagg tggatacacc ggaggctcac tttaccggta
     7141 atgtcacagt ggataagaac ctgcatgtca aaggcaatgt gtccattgac ggcaccggaa
     7201 gatcacaggg gacgttcacg atgtccgaag cggttattgc cgggatcacc tattcaggtc
     7261 atgtgcatca cgataacggt gaaggcagca aaacgggagg accagagaat ggctgatatt
     7321 gctgtcgtct gggatcaggg ttgcggttcc ctgcaactga acggcgcaga tcttctgacg
     7381 gataacagcc tgctgactgc ggtcattatt tcactgttta cggacaggcg ggcgctggat
     7441 tccgatgaaa tccctgacgg cacccgtgac cgtcggggat ggtggggaga cagtttccgg
     7501 gagcgccccg ttggctcccg tctctggctg ttaagccgtg aaaagacgct gtcctccgtg
     7561 gtcagccgtg cacaggccta tgctgatgaa gcgctggcgt ggctgcataa aagcggtgct
     7621 gccacatccg tggtatgtca tgccatgcgt gtggggcatg ctcgcctttc gctttccgtg
     7681 aaaatcaccc tgccggacgg aagcagacat ccgatgattt tttatgctga tatgaagggg
     7741 gaatgatgcc ttatcagccc ttaccactgg cgcaactgat cacacagaca caacaggata
     7801 tcagccagcg cctgcccggt tcgcagccgg gcgtgaatga aaccaccctg aatgccattg
     7861 cgtatgcact cgcgggtctg tcagcacagg agcatgaaca tctggcctgg atctcccggc
     7921 agataattcc gacagaagct gatgaagccg aactcctgaa acactgcgca ttctggggtg
     7981 tcatccgtaa accggcttcc cgcgctgacg gaccggtaca actgatgctg accacggatg
     8041 cagggatcac ggaaggcgta ctccttcagc gaagcgatgg tgttgtgtac cgcatcaccg
     8101 gctctgcgac cggaaaagcc ggaacactga atgttaatgt ggaggcggaa agtgcggggc
     8161 gcgctggaaa taccccgacc ggaacccgcc tgtcctttat cacgccacag gcgggcatca
     8221 accagacagc cacggtcacc ggcacgggac tcaccggtgg tgcggatgtg gaaacggtgc
     8281 cggagttgct gtccaggctg gtattccggg tacagaaccc gccatcaggg ggaacacagt
     8341 atgattttga acgctgggca cgggaagtac cgggcgtgac gcgggcatgg tgtaagcctg
     8401 aatggcctga ggcgggtagt gttggtgtga cttttgttca ggataataac cctgacattt
     8461 tccccggaga aggtgatgtg aagcgggtgg cggattatat ccgcagtcat gatgatccgg
     8521 cgacgggcca gcccgttggt cagccacttg ggccgacaat cagcgtgttt aagctgacca
     8581 ataagccggt ggcgtttgag atcaggattg tacccaaaac gccggaaaat caggctgccg
     8641 taaaacaggc attaacggac ctgctttata acgaatcgcg gccgggtgga cttgtattgc
     8701 cttcatcatt ctggcgggct gttgcagggg tgaaaggact ggaggatttt gaagttcgca
     8761 gcccgctgaa gtccgtgatg gccggagata cagagttgct gaccgtgggg gaaatcacat
     8821 ggctgtaacc ctgaccccgc atcagcgtgc cctgttgcag ttgctgcctg acgggctggc
     8881 atgggataag cggccgtcat ccgttcttgc ggctttgtgc ctgggcctca gtcattccac
     8941 ggagcgtgtt tcctggaccg gtaaccagat gctggcagaa cgttttcctg attcatcccg
     9001 tctgctgctg gaagactggg agcgttatct ggggttaccg gaatgtgata tgaccggcgc
     9061 aaccattcag gagcgtcagc gttatgccgg gaataaatac cggatgaaac cctctcttaa
     9121 ccgtgaattt tatatccggt ttgcggcaga gtttggttat gaaatagata ttcagccatc
     9181 accggattca cagtgggtca gtattgtcac gattaacagt gaaaccggct accggaatat
     9241 gaatgtgctg gatgatattc tcaccccgct gcgtatttat gagggtggtg cgctggaatg
     9301 tattctgaac cgttataagc ctgcatggca gacgtttatt tacgtgtatg caaacagcca
     9361 tgaagaggag actatttaat gtttcatgtt gataataatt ccggcgtggc gaatatgcct
     9421 gcgctggcac cggcgcagag taataccacc acctggttta ccgaaggtga cggacaaaaa
     9481 ggtatcagct ggattggtca ggactggctg aatattctcc aggccgaact gctgaatatt
     9541 ctggctgaag ccagtattca gccggataag gcgcagttaa accagcttac gctgtccatt
     9601 aaagccatta tcgctgcgaa tgccttttcc cggaaaaaca acctgaaaga aattgctgat
     9661 gccggtgcgg aagcccagcg tcttgcccgt agttatcttg gtcttggggc gcttgccaca
     9721 aaaaacagtc ttggtcccgg tgacgttaat gctctggcga aggatcagaa ccttgccgac
     9781 ctggagaatg cgggaaccgc ccgtaataat ctggatgttt acagcaaaag cgaaggtgat
     9841 aaccgttacc tgcgcaggga gcagaacggc gcagacattc cggataaagg cgcttttatc
     9901 gataacgtcg gtttacggga aacggtaaat aaggctgctg atgccctgcc atcgggtgga
     9961 accgccgtgg cagcaaacag actcgccacc ccaagaaata ttaatggcgt tccttttgac
    10021 ggaacgcagg atatcaacat cacgtcagga atgacgcagt caaccgcaga tggccggtat
    10081 gtacagaatg ttcagcttgg agcacagagc tatcattcac ccggaggcaa tgaaatatca
    10141 tggaattaca gcgcaccttc cggttgtatg ctttccggta ttaacgtgca ggaaaccggc
    10201 tcccggtctg cggacaatat cggtggggtc tattatcgcc cggttcagat ttatattaat
    10261 aatgcctgga gaacggtaag ttcagtgtaa accacagaaa agggtgctga atgcaccctc
    10321 tgaattattc cggtttttcc ggccagggaa tcgcctggta atgcccctca ctggcaatat
    10381 cattaaagtg catttcttta agctgtcgga tatacgccat ccagcgcgtc agctgtaatt
    10441 tattttcatc gctaatcatg tccagtttta attctgtttt ccagtcatcc acggtttcgt
    10501 tggcttcctg gagcagcgat gagcgacgct tttcagcaac cgtcttccag tttaccggca
    10561 cctttgatat caggccatca gaaaactgcc agttaccgtc aatattcaca ccttccggca
    10621 gttcgtccac ttcaacaaca gtaaaacctg ccggatataa agcagagaca tcttcagcaa
    10681 cagagcagat aat
//
LOCUS       DAZGSP010000147        10685 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZGSP010000147 DAZGSP010000000
VERSION     DAZGSP010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.148"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1204
                     /locus_tag="ACHK74_004587"
     CDS             <1..1204
                     /locus_tag="ACHK74_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFP0223290.1"
                     /translation="GTGQALSAAMNLTGTVTRDGIVYACVGGRRYTLPAPKGKKGKEL
                     TDELAALINADPDAPFTASSGAGSGDNGAGLKGSLGITARFTGECSVHDVRLNYHDGE
                     ATPEGIQVAIAYPKQKAANPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRW
                     GPAKMSDGVLWLAHTGTFGEVQAFGAKRNDFLATCTSIPKAPEPDYLWAAAVCATCAP
                     SLGADPARPLQTLALPSRMAPAPADRLTREERNSLLWGGIATVSVAAGDVLQIERQVT
                     MYRQNVYGESDPSYLDVETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVT
                     PEIMKLQLISLGEEWVELGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFL
                     AAQIRFIL"
     gene            1213..1578
                     /locus_tag="ACHK74_004588"
     CDS             1213..1578
                     /locus_tag="ACHK74_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFP0223291.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISAKFASATSTRVQ"
     gene            1593..2069
                     /locus_tag="ACHK74_004589"
     CDS             1593..2069
                     /locus_tag="ACHK74_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFP0223292.1"
                     /translation="MTTRKKKNAVSDEAVIEAIRDALEDGDPRTAGLAEQLAKGYVDL
                     LDGLPFGESREYRVTFRELTAKDSIDAETDAERLMETRNGPVLVASPALRGIALLRRQ
                     IAAVGDIQGPLSQRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRAGAVSGAD"
     gene            2197..4251
                     /locus_tag="ACHK74_004590"
     CDS             2197..4251
                     /locus_tag="ACHK74_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFP0223293.1"
                     /translation="MAKNLKASLIVDLLGNVSAKSRQWSQELGAFSRVGRTGLGGIGN
                     AARRAGQETELVGSRMQRTLAGVRGSIRTVTSDFDRLQGSITGTLGRLSNLYGMLAGG
                     AAVYGFNKAFIRPAAEMENYILRLNAINHGDTAKTEAVKAWAVQNAKETTWGLAGVMQ
                     EYASSRGFGMSDREARRFITMLQDQGGYHGWSLSDAQGASLQLKQMFARQSIQAADAS
                     ILTGYGINVYQLLADKLGVNQKTIREKGEKGKLGPDSIRLLFQVMAEQAKGAQKNAMN
                     SWTGMTSMMGDVWDGFAREVMAKGPFDSLKKSLKGFLDYADAAQKSGLQDKLATQTAS
                     ALNQGFEYARDAATGFYRAIQKVRETLQALRDAGYGDALDRIGQGAQTAAKYLLYMYL
                     ATRALKAVRAIGLGALRPGAALLGYGLSTAAVLTSPFRKPQTTVPGTQPGRGQRFMNF
                     LTGVNPAAVQPVLVTNWPAGGLASGGGDVVVSGDGKTVRSRKKRGPGRGRGVTTVVTA
                     GEQLAESAGKQGFFGRMMSRAGGLLSAAGSRMGLGRFAGLFRGAGRLGGGALWAGAMA
                     APVLLDSSASAVDKAGAVGSLAGSIAGGALGAAAGPVGVAIGSTVGSYLGDYLGGWLT
                     EAWQKLRGSDENGGQATAKTAARVELVAPEGWRARSIDVDDTAQHGLDVNVWNGGNYG
                     LY"
     gene            4238..5596
                     /locus_tag="ACHK74_004591"
     CDS             4238..5596
                     /locus_tag="ACHK74_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HFP0223294.1"
                     /translation="MASTDTPGRGSFRGVPFLVYQEQRERGGRNIVRREYPLRESGGA
                     DDLGPKLPEFTFTALVIGNDLQTQRSRLRDALRAPGAGELMHPDYGTLNVLINSFESR
                     YNASEQGTVEFTINVIPASDDTAPAVAEDTAAVLEQKSGSAMNQLFNTLSDGWTVISD
                     GLHDVQAMTDTISDKIDALENAVSGMGIVQDISAFTASFTALKGNAAALINSPRRMAE
                     SLAGMFAVLTGLPGDPSLSLTGKAATPSGSLTTNRDSMTEQAMPQLYRTLSSLRYTLS
                     GQDDPQILIGLTPAAQKNISLLRTVMQSATLVSQAQTVGKLLDQVVRQSATLVQDTDR
                     EGGLAWLESSADVQRINRDLSDALEQQVLDLSAQGHTGTALALRDARLALTEDLTTRS
                     VHLPGASQVMVRTTEPALVTLYRATGNSRRWQYFVRRNNIPDPVFVPGGRSVEVISEQ
                     HD"
     gene            5580..6704
                     /locus_tag="ACHK74_004592"
     CDS             5580..6704
                     /locus_tag="ACHK74_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFP0223295.1"
                     /translation="MSSMIELYIGGSIFSGWLTVSVRRSLEHLAGSFELGLMLPGERI
                     PSALRAGQSLTLRINGQTVISGWLDQVSQRISATRHQISISGRDKTGDLVDCAAIHPG
                     SQWRNRTLAQIAADLCAPFGIAVRWQVNDDTTARPFSSFTLENSETVADALTRAARHR
                     GVLVTSNADGDLVFTQAGNQQTDRLVLGENLLDADYNMDWRGRYSEYRVRGHGRGGGK
                     RGDSESAARLAAPVGIISDEQIGRYRPKIILADQQTDTSGARQRALREMRRAVARSER
                     FSATVRGWFRDDGRLWDVNLLTGVSALRFGIEQTELLVCQVEFLLDEQNGEVTRLVLA
                     PRDGFIVPAEPDSKGRGGSGDDVDAFIRQQMKKQGISFDE"
     gene            6694..7308
                     /locus_tag="ACHK74_004593"
     CDS             6694..7308
                     /locus_tag="ACHK74_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFP0223296.1"
                     /translation="MMNDEVFSRLIAPVTRGIRLLFGRGVLTGTHDELKMQNVQLTGM
                     DGETFDDVERPQQYGQISVPLPGAETFFACLGGQRDQTVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEATFTGNVTVDKNLHIKGNL
                     TIDGTGKSQGTFTMSEAVIAGITYSGHVHHDNGEGSKTGAPENG"
     gene            7301..7738
                     /locus_tag="ACHK74_004594"
     CDS             7301..7738
                     /locus_tag="ACHK74_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFP0223297.1"
                     /translation="MADIAIVWDQGCGSLQLNGADLLTDDSLMTAVLISLFTDRRALT
                     SDEIPDGTRDRRGWWGDSFRPRPIGSRLWLLSREKTLASVISRARAYADEALGWLSQD
                     GVASSVVCHAERVGHARLALSVRITLPDGSVRPMIFYADLKGE"
     gene            7738..8820
                     /locus_tag="ACHK74_004595"
     CDS             7738..8820
                     /locus_tag="ACHK74_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFP0223298.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYAQAG
                     LSAQEHEHLAWIARQIIPTEADEAELLKHCAFWGVMRKPASRGDGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITSSLTGKAGTLNVSVEAESAGRAGNAPAGTKLTFVTPQAGINQ
                     TATVTGTGITGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCRP
                     EWPQAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTVTVFK
                     LTNKPVPFSIRIIPKTPENQAAVKQALTDLLYNESRPGGLVLASSFWRAVAGVKNLED
                     FEVRSPLTSVQAGDSELLTVGEITWL"
     gene            8811..9371
                     /locus_tag="ACHK74_004596"
     CDS             8811..9371
                     /locus_tag="ACHK74_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFP0223299.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     AWTGKQLLAERFPDSSRLLLEDWERYLGLPECDMAGATITERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEENI"
     gene            9371..10282
                     /locus_tag="ACHK74_004597"
     CDS             9371..10282
                     /locus_tag="ACHK74_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0223300.1"
                     /translation="MFHVDNNSGVANMPELAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGADAQRL
                     ARGYLGLGTLATKNSLGPGDVNALAKVQNLADLENKGTARNNLDVYSKSEGDNRYLRR
                     EQHGADIPNKGAFIDNLGLRETVNRAANALPSNGTAVAANKLSAARKINGVAFDGTKD
                     ITITSGMTQATADGRYVRNVQLGAQSRHSPAGNEVTWDYKTPAGCMLSGIHVQDTGSR
                     SADNIGGVYYRPVQIYINNVWRTVSSV"
     gene            complement(10317..>10685)
                     /locus_tag="ACHK74_004598"
     CDS             complement(10317..>10685)
                     /locus_tag="ACHK74_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0223301.1"
                     /translation="IICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISK
                     VPVNWNTVAENRRSSLLQEANGTVDDWKTELKLDMISDENKLKLTRWMAYIRQLKEMH
                     LNDIASERHYQAISWPEKPE"
BASE COUNT         2347 a         2793 c         3278 g         2267 t
ORIGIN      
        1 tggcaccgga caggcgttat ccgccgccat gaatctgacc ggaacggtca cccgtgacgg
       61 aattgtttat gcctgcgtgg gggggcggcg ttatacactc ccggcaccaa aaggtaagaa
      121 aggcaaagaa ctgaccgacg agctggccgc gctgatcaat gccgatcctg acgcgccctt
      181 cacggcctcc tctggtgcag gaagtggtga taatggcgca ggtctgaaag ggtctctggg
      241 gatcaccgcc cgctttaccg gtgaatgctc cgttcatgat gtgcgcctga actatcacga
      301 cggggaagca accccggaag gaatacaggt cgctatagcc tatccgaaac aaaaagcggc
      361 taacccggat atcacccgaa gcgtggcggg tatgggcgat cgccagtaca actatgtcgt
      421 catgccctat aaggatgatg caaacctgaa aatcatcagt gatgagcttc tgaaacgctg
      481 gggaccggcc aaaatgtctg acggcgtgct gtggctggca cataccggca cgtttggtga
      541 ggtgcaggca ttcggggcaa aacgcaatga ttttctggcg acctgcacat ccattcccaa
      601 agcccctgaa ccggattacc tctgggccgc ggcggtctgc gccacctgtg caccctctct
      661 gggagcagat cctgcgcgtc cgcttcagac gctggcgctt ccgtcacgca tggccccggc
      721 tccggccgat cgtctgacgc gtgaagagcg taacagtctg ttgtggggag ggattgccac
      781 ggtcagtgtg gctgccggtg atgtgcttca gatcgaacgt caggtgacca tgtatcgcca
      841 gaacgtgtac ggcgaaagtg atcccagcta tctggatgtg gaaaccatct acaccctctc
      901 ctggctgcgt tattccctgc gtacttttat cacacaacgt tttccgcgcc ataaactggc
      961 tgatgacggg acgcccgttc gtgccggtca gaatatcgtg acaccggaga tcatgaagct
     1021 tcagttaatt tcgctgggtg aagagtgggt ggaactcggg ctggtcgaaa atctggacac
     1081 ttttaaaaag aatctgcttg tggagcgtaa tgcgtcagat cgaaaccgcc ttgacgtgtt
     1141 atgcacgccg gatctggtta accagttccg cttcctggca gcacagatcc gtttcatttt
     1201 gtgaggtaag gcatgagcgg aaaacagtat cagggcacgg ccactatccg tgtgaacggt
     1261 caggaatacg agacgcttga gggggctaca ttttctccgt caggttttga acgtgaagtg
     1321 gtgaaaggcg cgaaagtcta tggttaccgc cagaaaccac gtgaagcgac gctggactgc
     1381 aaatttccgg caggcggaga aggctcacca gccgctgatg aaatcaacaa ctggactgcg
     1441 gtcaccatcg agtttgtggc ggataccggc gaagtccaca tgatgacgaa ggcctggagc
     1501 agtgaaccgg cctcgcttga cggtggcggg gagatctccg cgaagttcgc cagtgccacc
     1561 agtacccgtg tccagtaatc aggaaaaatg caatgaccac acgaaagaaa aaaaacgcgg
     1621 tttctgacga agccgttatt gaggctatcc gtgatgcact tgaggacggc gatccacgca
     1681 ccgccggtct ggcagaacaa ctggcgaaag gctatgtgga tctgcttgat ggtctgccat
     1741 tcggtgagtc ccgtgaatac cgcgtcacct tccgcgaact gacggcgaaa gacagcattg
     1801 atgcggaaac cgatgctgaa cgtcttatgg aaacccgaaa cggcccggtt ctggtggcat
     1861 caccggccct gcgcggtatt gcgctgctgc gtcgccagat tgccgctgtc ggtgacattc
     1921 aggggccgtt gtcacaacgc cagattgggc agttaagcga gcgcgatctc tcccgtctga
     1981 tggcagcggt cagcctgctg gataccgcgc ttgccggaaa gctggcggct gatcgggggc
     2041 gagcaggcgc agtgtcgggc gcagattgaa gaagcggcaa tagtgctggg gatggtgacc
     2101 agaagcggcc cggaatgggc gcttaacctc cccttatcgc aactttaccg gcactgccaa
     2161 caaacggaaa aaattctcag aacgaagaag taatccatgg caaaaaacct gaaagcctcc
     2221 ctgattgtcg atttactcgg caatgtttcc gccaaatccc gccagtggtc acaggagctg
     2281 ggggcgttct cccgcgtcgg caggacgggg ctgggtggta ttggaaatgc agcccgccgt
     2341 gccggtcagg agacagagct tgtcggctcc cgtatgcagc gtacgcttgc cggtgtccgt
     2401 ggcagtatcc gcacggtaac gtcggatttt gaccgtcttc agggcagtat taccggcacg
     2461 ctgggacgtc tcagcaacct ttacgggatg ctggcaggag gggctgcggt ctacggtttt
     2521 aataaagcct ttatccgccc ggccgcagag atggagaact atattctgcg tctcaatgcc
     2581 attaatcatg gcgatacggc aaaaacggaa gcagtgaaag cctgggcggt acagaacgca
     2641 aaggaaacca cctggggact ggcgggggtt atgcaggaat acgcctccag tcgcgggttc
     2701 ggtatgagtg acagggaagc ccgccgcttt attaccatgc ttcaggatca gggcggctat
     2761 cacggctggt cactgtcaga tgcacagggg gcgtccctgc aactcaaaca gatgtttgcc
     2821 cgccagagta tccaggccgc ggacgccagt atcctgaccg gatacgggat aaacgtttac
     2881 cagttgctgg ccgataaact gggtgtaaac cagaagacca tccgtgagaa aggcgagaaa
     2941 ggaaaactgg gaccggacag cattcggctg ttatttcagg tgatggccga acaggcaaaa
     3001 ggtgcccaga aaaacgccat gaactcatgg acagggatga catccatgat gggcgacgtc
     3061 tgggacggat ttgcccgtga agtaatggca aaagggccat ttgacagcct gaaaaagagc
     3121 ctgaaaggct tcctggatta tgccgatgcg gcacagaaaa gcggcttaca ggacaagctg
     3181 gccacacaaa cagcctcagc cctgaatcag ggctttgagt atgccagaga cgcagcgacg
     3241 ggtttttacc gggccattca gaaagtcagg gaaacgcttc aggcattgcg tgacgctggt
     3301 tatggcgatg cgctggatcg cattggtcag ggcgcacaga ccgccgcgaa atatctcctg
     3361 tatatgtatc tcgccacccg tgcgctgaag gctgtccgcg ccataggtct tggggcactg
     3421 cgaccggggg cggcattgtt ggggtacggg ttatccacgg ccgctgtgct gacctcgcct
     3481 tttcgtaaac cgcagaccac cgttccgggc acgcagccag gacgcggcca gcgttttatg
     3541 aactttctta ccggggtaaa tcctgctgcc gttcagcccg tactggtcac caactggcct
     3601 gcgggtggtc tggcatctgg tggcggggat gtggtggtca gcggtgacgg caaaaccgtg
     3661 cgcagccgta aaaaacgcgg ccccggacgt gggcggggtg tcacgaccgt tgtgactgcc
     3721 ggtgagcaac tggcagagag cgccggtaaa cagggcttct tcggtcgtat gatgagccgt
     3781 gccggtggtc tgctttcggc tgccggaagc cgcatggggt tggggcgttt tgccgggctt
     3841 ttccgtggtg ccggtcggct gggcggtggt gccctgtggg ccggggccat ggcggctcct
     3901 gtactgctgg acagcagtgc cagtgccgtt gataaagccg gtgccgtggg ttcccttgcc
     3961 ggaagtatcg ccggtggtgc gctgggggct gccgccggtc cggtcggggt tgccattggc
     4021 tccacggtgg gcagttatct cggtgattat ctggggggat ggctgaccga agcctggcag
     4081 aaattacggg gcagtgatga aaatggcgga caggccaccg cgaaaaccgc cgcacgggtg
     4141 gagcttgtgg cccctgaagg ctggcgggca cgcagtattg atgtggatga taccgcacag
     4201 catggtcttg atgtgaacgt ctggaacgga gggaactatg gcctctactg acacgccggg
     4261 gcgtggctcc ttccgtggtg tgccgttcct ggtttatcag gagcagcggg aacggggcgg
     4321 acgtaacatt gtgcgccgtg aatatccgct gcgtgaaagc ggcggtgcgg atgatttggg
     4381 gccaaaactc ccggagttta cattcacggc tcttgtgatc ggcaatgacc ttcagacgca
     4441 gcgaagccgc ctgcgggatg cgctgcgggc acccggtgcc ggtgaactga tgcacccgga
     4501 ttacggcacc ttaaatgtgc tgataaacag ctttgaaagc cgttataacg ccagcgaaca
     4561 gggaacggtt gagttcacga tcaacgtgat ccccgccagt gacgataccg caccggcggt
     4621 ggctgaagac acggctgccg ttctggagca gaaaagcggt tcagcaatga atcagttgtt
     4681 taacacgctt tcagacggct ggacagtgat ttcagacggc ctgcatgatg tgcaggccat
     4741 gaccgacacc atcagcgata aaattgatgc gctggagaat gccgtttcgg gtatggggat
     4801 tgtgcaggat atcagtgcct ttacggccag tttcactgcc ctgaaaggga atgctgccgc
     4861 cctgattaat tcgccccgtc gcatggctga atctctggcc ggaatgtttg cggtgctgac
     4921 gggactgccc ggcgatcctt ccctgtcact gaccgggaag gccgcaacgc cgtccggcag
     4981 cctgaccaca aaccgtgaca gcatgacaga gcaggcaatg ccgcaactgt accgcacact
     5041 ttcttccctg cgttataccc tcagcgggca ggatgatccg cagatcctga ttggtctgac
     5101 gcctgcggca cagaaaaata tcagtcttct gcggacagtc atgcagagcg caaccctggt
     5161 gtcacaggcg cagacggtag gaaaactgct ggatcaggtc gtccggcaga gtgcgacgct
     5221 cgttcaggat actgaccggg agggcggact ggcctggctg gaaagttctg ccgatgtgca
     5281 gcggatcaac cgggatttga gtgatgccct ggaacaacag gtgctggatt tgtccgcaca
     5341 gggccatacc ggaaccgccc ttgcattacg tgatgccaga cttgcgctga cagaggattt
     5401 aacaacgcgt agcgtgcatc ttcccggcgc ttctcaggtc atggtgcgaa ccaccgaacc
     5461 ggcactggtc acgctgtacc gtgccaccgg caacagccgt cgctggcagt attttgtccg
     5521 caggaacaac atccctgatc cggtttttgt tcctggtggt cgcagtgtgg aggtgatcag
     5581 tgagcagcat gattgagctg tatattggcg gcagtatttt ttccggctgg ctgacggtca
     5641 gtgttcgtcg ctcgctggag catctggccg ggtcgtttga actggggctg atgctgcccg
     5701 gtgagcgcat cccctcagcc ctgcgggccg gtcagtccct gacgctcaga attaacgggc
     5761 agacggtcat cagtggctgg ctggatcagg tcagtcagcg gatcagtgca acccgtcatc
     5821 agatcagcat cagcggtcgg gataaaaccg gcgacctggt ggactgtgcc gccatccatc
     5881 ccggcagcca gtggcgaaac cggacgctgg cacagattgc cgcagattta tgtgcgccct
     5941 tcggaatagc cgtgcgctgg caggttaacg acgacacgac tgcacgcccc ttcagctctt
     6001 ttaccctgga aaactcagaa accgtggcgg atgcgctgac ccgtgcggcc cggcatcgtg
     6061 gggtgctggt gaccagtaat gctgacggcg atctggtgtt cactcaggca ggaaatcagc
     6121 agacggacag actggtgctg ggggaaaacc tgcttgatgc cgattacaac atggactggc
     6181 gaggacgata cagcgaatac cgtgtccggg ggcatggacg cggtgggggt aaacggggag
     6241 acagcgaatc cgccgcccgg ctggcggccc cagtgggcat catcagtgat gagcagatcg
     6301 gtcgctaccg gccgaaaatc atcctcgccg atcaacagac agacacctcc ggtgcacggc
     6361 agcgcgccct gcgggagatg cgccgtgcgg ttgcccgttc agaacggttt tccgccaccg
     6421 tgcgtggctg gttccgggat gatggccggt tatgggatgt caatctgctg accggtgttt
     6481 cggccctgcg tttcggtatc gaacagactg aacttctggt ctgccaggtg gagtttttac
     6541 tggatgagca gaacggggaa gtcacccggc tggtactggc accgcgtgac ggctttatcg
     6601 ttccggcaga gccggacagt aagggtcgtg gtggttccgg tgatgatgtt gatgccttta
     6661 ttcgccagca gatgaaaaaa caggggatca gctttgatga atgatgaagt gttcagccgc
     6721 ctgattgccc cggtaacgcg cggcattcgc ctgctgtttg gccgcggcgt tctgaccggc
     6781 acacatgacg aactgaaaat gcagaatgtg cagctcaccg gcatggacgg cgaaaccttt
     6841 gatgatgtgg agcgtcccca gcagtacggg cagatcagcg ttcccctgcc gggtgcggaa
     6901 accttttttg cctgtctggg cgggcagcgt gatcagaccg tggtgcttgt ggtggaagac
     6961 cgccgcagtc gcccgaccgg actcactgcc ggagataccg gcgtgtatca ccatgagggg
     7021 caccgcatac gtctgacaaa gaacggtcgg atcattgtga cctgtaagac gctggaaatt
     7081 tacgccgatg aaggtgttca ggtggataca ccggaagcca ccttcacggg caatgtcacg
     7141 gtggataaaa acctgcatat caaaggtaat ctgaccattg atggcacagg aaaatcacag
     7201 ggtacgttca cgatgtccga ggctgttatt gccgggatca cctattccgg ccatgtacat
     7261 catgacaacg gtgaaggcag taagacagga gcgccggaga atggctgata ttgcaattgt
     7321 gtgggatcag ggatgcggtt cgctgcaact gaacggtgca gacctgctga cagatgacag
     7381 tctgatgaca gcggttctga tttcactgtt cactgaccgc cgggcactga cgtcggatga
     7441 aatccctgat ggcacacgtg accgccgggg atggtgggga gacagttttc gcccgcgccc
     7501 cattggctcc cgtctgtggc ttctcagtcg ggaaaaaacg ctggcctccg tgataagccg
     7561 tgcccgtgcc tacgcggatg aggcgctggg ctggctcagt caggatggcg tggcgtcatc
     7621 cgtggtctgt catgcagaac gtgtggggca cgcgcggctg gcgctttcgg tgcgcataac
     7681 cctgcctgat ggttcggtaa gacccatgat tttttatgct gatcttaagg gggagtaatg
     7741 ccttatcagc ctttaccgct ggcacagtta atcacgcaga cacagcagga tatcagccag
     7801 cgtcttcccg gttcgcagcc gggcgtgaat gaaaccaccc tgaatgccat tgcgtatgct
     7861 caggcaggat tgtctgcaca ggagcatgaa catctggcct ggattgcacg tcagatcatc
     7921 ccgacagaag ccgatgaagc cgagttgctg aagcactgcg ctttctgggg tgtcatgcgt
     7981 aaacctgcct cacggggtga cgggccggtt cagctgatgc tgaccacgga tgcggggatc
     8041 acggaaggtg tgcttctcca gcgcagtgac ggcgtggttt accgcatcac ctcctcactg
     8101 accggtaaag ccggtacgct gaatgtcagt gtggaggccg aaagtgccgg tcgtgcggga
     8161 aatgccccgg cagggacgaa actgacgttt gtcactccgc aggcggggat caaccagacg
     8221 gccacggtga ccggaacagg gatcaccggt ggtgcggatg tggaaacggt gccggagctg
     8281 ctttcccgcc tggttttccg ggtgcagaat ccgccgtccg gcggtacaca gtacgatttt
     8341 gaacgctggg cgcgtgaagt gccgggggtg acgcgggcat ggtgccgccc ggaatggccg
     8401 caggcgggca gcgtgggggt gacgttcgtt caggacaaca acccggacat ttttcccggt
     8461 gaaggtgatg tgaaacgtgt ggcggattat atccgcagtc atgatgatcc ggcgaccggc
     8521 cagcctgtcg gtcagcctct ggggccgacg gtgaccgtgt ttaaactgac caataagcca
     8581 gtgcctttca gtatcaggat catcccgaaa acaccggaga atcaggcggc agtaaaacag
     8641 gcactgaccg acctgttata caacgaatcc cgccccggcg gtctggttct ggcgtcgtct
     8701 ttctggcgtg ccgtggcggg ggtgaaaaat ctggaggatt ttgaagtgcg cagcccgctg
     8761 acgtcggttc aggcgggaga cagcgagctg ctgacagtgg gggaaatcac atggctgtaa
     8821 ccctcactcc gcatcagcgc gccctgttgc agttgctgcc tgacggactg gcctgggaca
     8881 agcggccgtc atccgtactg gctgcgttat gccttggcct cagccattcc accgaacgtg
     8941 ttgcatggac gggaaaacag ctgctggcag aacgtttccc tgacagttcc cgtctgcttc
     9001 tggaagactg ggagcgttat ctcggccttc ccgagtgtga tatggccgga gccaccatca
     9061 cagaacgaca gcgttatgcc gggaataaat accggatgaa gccttcgctg aaccgtgaat
     9121 tttatatcag gtttgcggca gagtttggtt atgaaataga tattcagcca tcaccggatt
     9181 cacagtgggt cagtattgtc acgataaaca gtgaaaccgg ctaccggaat atgaatgtgc
     9241 tggatgatat tctcacgccg ctgcgtattt atgagggtgg tgcgctggaa tgtattctga
     9301 accgttataa gcctgcatgg cagacgttta tttacgtgta tgcaaacagt cacgaagagg
     9361 agaatattta atgttccatg ttgataataa ttccggcgtg gcgaatatgc ctgaactggc
     9421 tccggcgcag agtaatacca ccacctggtt taccgagggg gacggacaga aaggtatcag
     9481 ctggattggt caggactggc tgaatattct ccaggccgaa ctgctgaata ttctggctga
     9541 agccagtatt cagccggata aggcgcagtt aaaccagctt acgctgtcca ttaaagccat
     9601 tatcgctgcg aatgcttttt cccggaaaaa taacctgaaa gaaattgctg atgccggtgc
     9661 ggatgctcag cgtctggccc gtggttatct tggtctgggg acgcttgcca caaaaaacag
     9721 tcttggtccc ggtgacgtta atgccctggc gaaggttcag aatctcgccg acctggagaa
     9781 taagggaacc gcccgtaata atctggatgt ttacagcaag agcgaaggtg ataaccgtta
     9841 cctgcgcagg gagcagcacg gcgcagacat tccgaataaa ggggctttta tcgataacct
     9901 cggtttacgg gaaacggtaa acagggctgc aaatgccctg ccatcgaatg gtacagccgt
     9961 agcggcaaac aagctgtctg cggcacgtaa aattaatggt gttgcgttcg atggcacaaa
    10021 agacatcact attacatccg gtatgaccca ggctacagca gacggcagat atgttcgtaa
    10081 tgtgcaactt ggtgctcaga gccgccactc tcctgcgggt aatgaagtca cctgggatta
    10141 taaaacacca gcaggttgta tgctttccgg tattcatgta caggataccg gctcccggtc
    10201 tgcggataat atcggtgggg tctattatcg cccggttcag atttatatta ataatgtctg
    10261 gagaacggtg agttcagtgt aaaccacaga aaagggtgct gaatgcaccc tctgaattat
    10321 tccggttttt ccggccagga aatcgcctgg taatgcctct cgctggcaat atcattaagg
    10381 tgcatttctt taagctgtcg gatatacgcc atccagcgcg tcagctttaa tttattttca
    10441 tcgctaatca tgtccagttt taattctgtt ttccagtcat ccacggttcc gttggcctcc
    10501 tggagcagcg atgaacggcg attttcagca accgtattcc agtttaccgg cacctttgat
    10561 atcaggccat cagaaaactg ccagttaccg tcaatattca caccttccgg cagttcgtcc
    10621 acttcaacaa cagtaaaacc tgccggatat aaagcagaga catcttcagc aacagagcag
    10681 ataat
//
LOCUS       DAZGSP010000148        10628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZGSP010000148 DAZGSP010000000
VERSION     DAZGSP010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.149"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            112..513
                     /locus_tag="ACHK74_004599"
     CDS             112..513
                     /locus_tag="ACHK74_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223302.1"
                     /translation="MDKENSSIPERWFIASGVAVILLTLLAFMSFPEPDVSMNRYYSW
                     LMNVLILSVTGVMYVALSVVMVMLYFLYERNSRLLYLSVLALIIAAGIWLLRFLDDFL
                     MTPAFHVDLFVLWVLLCFLYIRCKNEMSIPE"
     gene            579..860
                     /gene="traQ"
                     /locus_tag="ACHK74_004600"
     CDS             579..860
                     /gene="traQ"
                     /locus_tag="ACHK74_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001732192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFP0223303.1"
                     /translation="MSKYKFSLPRLDITGVWVFSLGLWFHIVARLVYRKPWMAFFLAE
                     LIAAILVLFGAYQILDTWIARVSREEREALEARQAAILAAEKESRHVSH"
     gene            847..1401
                     /gene="trbB"
                     /locus_tag="ACHK74_004601"
     CDS             847..1401
                     /gene="trbB"
                     /locus_tag="ACHK74_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFP0223304.1"
                     /translation="MSLTKSLLFTLLLSAAAVQASTRDEIERLWNPQGMAAQPAQPAA
                     DTSARTDKTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYG
                     FSVFPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGA
                     TDAAGFMARMDTVLQMYGGKTGAK"
     gene            1391..1705
                     /gene="trbJ"
                     /locus_tag="ACHK74_004602"
     CDS             1391..1705
                     /gene="trbJ"
                     /locus_tag="ACHK74_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFP0223305.1"
                     /translation="MRNKQVVLLIAGISGVATGIIVSLNIPFIRQGLFYPASPVEIVV
                     SLCLTFSVSVVFFVGAIVGWILVSEIYYSRMTGLNESSEISEETYNERKQKESGIQNQ
                     KN"
     gene            1659..2051
                     /gene="trbF"
                     /locus_tag="ACHK74_004603"
     CDS             1659..2051
                     /gene="trbF"
                     /locus_tag="ACHK74_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFP0223306.1"
                     /translation="MKENKKNQEFKIRKIKRDIERSCDNAIKYFWLFVVFFAAGLIVR
                     NVMHDFFSAGIDSWKADPELNNFRYMWNTLMYVIPIMLYALAAGFLAAASLSPLCEII
                     FGGVRIFLLKRRMRRENTLREGSNNASH"
     gene            2038..3411
                     /gene="traH"
                     /locus_tag="ACHK74_004604"
     CDS             2038..3411
                     /gene="traH"
                     /locus_tag="ACHK74_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFP0223307.1"
                     /translation="MLRIKPLFILCAALLTVTPAASADVNSDMNQFFNKLGFATNTTQ
                     AGVWQGQAAGYAYGGSLYARNQVRNIQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAMGYFFDLALQTTVPEIKTAKDFLQKMANDINSMNLSSCQVAQGIVGG
                     LFPQTQESQQKVCQDIAGESNIFADWAASRQGCTVGGQSSAVRDKASDKDKERVLKNI
                     NIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDIVRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTISKDRALKSQIAKLLTSIQNKALSDSPLAENEKTF
                     IQSTTIPVFRYLVDPLMLGVSNSVLYQLTDYIGYDIMLQYIQELLQQARAMISTGNYP
                     QAVLDSVLDNLNQAQQQIAIFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGGVQ"
     gene            3408..6230
                     /gene="traG"
                     /locus_tag="ACHK74_004605"
     CDS             3408..6230
                     /gene="traG"
                     /locus_tag="ACHK74_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFP0223308.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVQVHRVDNVPVGLAMPL
                     SLTTRIGHAMVAGYEMVFAQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMVADDPYMVIFSRPSPLRGVYDSNNNFVSCKDASVTLK
                     DKLNLDTKTGGKTWHYYVQQLFGGRPDPDLLFRELLSDSYSYFYGASQSASQIMRKNV
                     TINALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSVGHVIMRNLPMVQTILTG
                     ITIGIFPLLVLAAAFNKMTLPVLKGYVFALMWLQTWPLLYAILNSAMTFYAKQNGAPV
                     VLSEVSQIQLKYSDLASTAGYLSAMIPPLSWMMVRGLGAGFSSVYSHFASSSITPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGSMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMARESMNKAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTSGADSTMSAQDSMMASRMRSAVESYAKAHNISDEQATQELASRSTRASAGMY
                     GDAHAEWGIKPKILGVGGGAGVRGGGRAGIDWSDDDAHQASSGSRASHDARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQGIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSILGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSA
                     QERMPGADSPQELMKRAKEYQDKHKQ"
     gene            6246..6743
                     /locus_tag="ACHK74_004606"
     CDS             6246..6743
                     /locus_tag="ACHK74_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein"
                     /protein_id="HFP0223309.1"
                     /translation="MMMKNSAHIALITVIQLIACYLADWGSAETVFILCFIVLWQGLF
                     IWLFSQIRKKRHVSDEFKFSTGVWYITMPVSSLLSPLLSLMVFIIGTLYELRRVSGCV
                     SVREWGGNQLKNQYDGGEKLEFGFVEYTPTTYYNPSTGYPMHGGFDSAGNTFGTRWQD
                     YDDRR"
     gene            6775..7506
                     /gene="traT"
                     /locus_tag="ACHK74_004607"
     CDS             6775..7506
                     /gene="traT"
                     /locus_tag="ACHK74_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFP0223310.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPSSERTVFLQIKNTSDKDMSGLQAKISDAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQSWLNSGYEGAIAGAALGTGITGYNSSSAGAALGVGIATGLIGMAADAMVE
                     DINYTMITDVQIAERTTSKVRTDNVAALRQGTSGAKVQTSTSTGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            7566..7826
                     /locus_tag="ACHK74_004608"
     CDS             7566..7826
                     /locus_tag="ACHK74_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223311.1"
                     /translation="MNRAESRLSQVLFLLGYDSEKYPEHVCRELLKELYRFADATGDS
                     ELAIACQRYLTALEPFSAYEIGVSATEYLPVSEYPTGRRRRK"
     gene            7994..8725
                     /locus_tag="ACHK74_004609"
     CDS             7994..8725
                     /locus_tag="ACHK74_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223312.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTATTKDSLYSNNI
                     DTDLLVVIGGLVVILFLMMLVMYFINSLRHKELTEILTKISFPEKSGSNPLKPEEGRC
                     NLLSLCVLAPVFCTPKKLAEVSRQIIVTSAQEIAWTPGSSRIDGEQEENITWSLSSQT
                     HADKYDLINKTSRVSPDSGHFYEPGTALDYRERVRLFIQIQFSHESDWSEILRLTDIV
                     LEKVSSGRSAGGYHHRYTGYTWFVL"
     gene            8781..>10628
                     /gene="traD"
                     /locus_tag="ACHK74_004610"
     CDS             8781..>10628
                     /gene="traD"
                     /locus_tag="ACHK74_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFP0223313.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYARKRGDMVVI
                     YDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTYLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHSGESFTIRDWMRGVREDKQNGWLFISSNADTHA
                     SLKPVVSMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQA"
BASE COUNT         2682 a         2425 c         2829 g         2692 t
ORIGIN      
        1 tgaactcatt tacagagtaa aagacaatca gagataactt ttttctgtcc ccacacctga
       61 tggtgaatgg cttttaattc atgttgtttg ttaagtatgg ttttattctg tatggataaa
      121 gaaaatagta gtattcctga aagatggttt atcgcgtctg gtgttgcggt tattttactg
      181 accttactgg cttttatgag ttttccggag ccggatgtca gtatgaatcg ctattatagc
      241 tggttgatga atgtgttaat tctgagtgtg accggggtaa tgtatgtggc gctgtctgtt
      301 gtaatggtaa tgctttattt tctttacgag cgtaacagca ggttattgta cttgtctgtg
      361 cttgccctaa tcattgcagc aggtatctgg cttctcagat ttctggatga ttttttaatg
      421 acgccagcat ttcatgttga tctgtttgtt ttatgggtgc tcctctgttt tttgtacatt
      481 cgctgtaaaa atgaaatgag tataccggaa taataatcag tcggtttttt atttgccatc
      541 ctgctgagtg tggaagatat tacaaacagg atatatttat gagtaaatat aaattctctt
      601 taccccgcct tgatattaca ggtgtatggg tgttctccct ggggctctgg tttcatattg
      661 ttgcccgtct ggtttacaga aagccctgga tggctttttt tctggcagag ctgattgcgg
      721 ccattctcgt tctgtttggc gcataccaga tactggatac atggatagcc cgcgtcagcc
      781 gggaagagcg tgaggcactg gaagcccgtc aggccgccat tcttgctgca gaaaaggaga
      841 gcagacatgt ctctcactaa atcactgctg ttcaccctgt tactgtcagc cgctgccgtg
      901 caggcctcca cccgggatga aatagagcga ctctggaatc cgcagggtat ggccgcacag
      961 cctgcacaac cggcagcaga tacgtcagcc aggacagaca agacagaaaa gccggctccc
     1021 cgctggttcc gcctcagcaa cggcaggcag gttaacctgg ccgactggaa ggtggtgctg
     1081 tttatgcagg ggcattgccc ttactgtcac cagtttgacc cggtactgaa gcagctggca
     1141 cagcagtacg gcttttccgt ttttccctac accctggatg gtcaggggga taccgccttt
     1201 ccggaagcat taccggtgcc accggacgtg atgcagacct tcttcccgaa tattccggtg
     1261 gccacaccga ccaccttcct ggtcaacgtc aacacgcttg aggcattacc gcttttacag
     1321 ggggcaacgg atgccgccgg ttttatggcg cggatggata ccgttctgca gatgtacgga
     1381 gggaaaacag gtgcgaaata agcaggtggt gttacttatt gccggaatat ccggcgtcgc
     1441 gacgggaata atcgtcagcc tgaatatccc ctttatccgt caggggcttt tttatcccgc
     1501 cagccctgtg gaaattgtcg tttcgctgtg tcttaccttt tctgtttctg ttgtgttttt
     1561 tgtaggggca attgtgggat ggattttggt gtctgaaata tattacagcc gcatgaccgg
     1621 tctgaatgaa tcatcagaaa tatcagagga aacttataat gaaagaaaac aaaaagaatc
     1681 aggaattcaa aatcagaaaa attaaacggg atattgagcg aagttgtgat aacgccataa
     1741 aatacttctg gctttttgtg gttttctttg ctgccggatt gattgtaagg aacgttatgc
     1801 atgatttttt cagtgccggt attgattcgt ggaaagctga cccggaatta aataattttc
     1861 gttatatgtg gaacaccctg atgtatgtca ttccgattat gttatatgcg cttgcagctg
     1921 gttttcttgc tgcggcttct ctgtcgccgt tgtgcgaaat aatatttggg ggtgtccgga
     1981 tattcctgct gaaacgccgt atgcgccggg agaacacatt aagggagggc agcaataatg
     2041 cttcgcatta agcctctttt tattctttgt gccgccttac tgacggtcac acctgctgcg
     2101 tcagcggatg tgaacagcga catgaaccag ttcttcaaca agctgggctt tgccaccaat
     2161 accacccagg ccggagtctg gcaggggcag gctgccgggt atgcctatgg cggttctctg
     2221 tatgcccgta atcaggtcag aaatatccag cttatctcca tgacactgcc cgacatcaat
     2281 gcagggtgtg gtggcatcga tgcctatctc ggctcattca gttttattaa tggcgagcag
     2341 ctgcagcgtt ttgttaagca gattatgagt aacgcgatgg ggtacttttt tgaccttgcc
     2401 ctgcagacaa cggtgcccga aatcaaaacc gcgaaagact tcctgcagaa gatggcgaat
     2461 gacattaaca gtatgaacct cagtagctgt caggtggccc aggggatagt tggcgggttg
     2521 tttcctcaga cgcaggagtc gcagcagaag gtctgccagg atattgcagg tgagagcaat
     2581 atcttcgctg actgggccgc ttcccgtcag gggtgcacgg tcggagggca gtcttctgcg
     2641 gtgcgtgaca aggccagcga taaggacaag gagcgggtac tgaaaaacat caatatcatc
     2701 tgggactcgc tgtccaaaaa caggatgctt gatggtaaca aagagctgaa agagttcatt
     2761 atgacgctca caggtacgct gatttttggt gaagacagtg agattacccc tcttcctgca
     2821 cgaaccactg accgcgatat tgtccgagcg attatggaag ggggtaccgc gaaaatttat
     2881 cactgcaatg attcggataa gtgcctcaaa gtggtggccg atgcgaatgt gaccatcagc
     2941 aaggacaggg cgctcaagtc acagatagcg aaactgctca ccagtattca gaacaaggcg
     3001 ctcagtgatt ctccgctggc tgagaacgaa aaaaccttca ttcagagcac caccattcct
     3061 gttttcagat atctggttga cccactgatg ctgggcgtat ccaacagtgt tctctaccag
     3121 ctgacggact atatcggtta cgacatcatg ctccagtaca ttcaggaact gcttcagcag
     3181 gcccgggcga tgatttccac cgggaactac ccgcaggcgg tcctggacag tgtgctcgat
     3241 aaccttaatc aggcgcagca acagattgcc attttccaga gccaggtgca ggtccagcag
     3301 gatgcattgc tggtcgttga ccgtcagatg agctacatgc gccagcagtt gtctgcccgt
     3361 atgctcagcc gttaccagaa taattatcac ttcgggggag gcgtacagtg aacgaggtct
     3421 atgtcatcgc cggtggggaa tggttacgga acaacctgaa tgccatcgct gcctttatga
     3481 gtacccggac gtgggattcc atcgaaaaaa ttgcgctcac attatctgtt ctcgcagtgg
     3541 ctgtgatgtg ggtacaacgg cacaacgtga tggatttgct gggatgggtt gccgtgtttg
     3601 tacttatcag tctgctggtt aatgtccgca catcggtgca gattattgat aacagtgacc
     3661 tggttcaggt ccatcgtgtg gataatgtac cggtcgggct ggcgatgcca ctttcactga
     3721 cgacccgtat cgggcatgcg atggtggccg gctatgagat ggttttcgct cagccggaca
     3781 gcgccaccta cagcaagaca ggcatgctgt tcggggcgaa cctgattgtg aaaagcaccg
     3841 atttcctgtc ccggaatccg gaaatcatca atctgtttca ggactacgtc cagaactgcg
     3901 tgctgggtga tatttacctg aaccacaaat acacgctgga agacctgatg gtggctgatg
     3961 acccctatat ggtgattttt tcccggccca gcccgctgcg gggggtatat gacagtaata
     4021 ataattttgt cagctgcaag gatgcgtcgg tcacgctgaa agacaagctg aatcttgata
     4081 caaagaccgg aggcaagacc tggcattatt atgtgcagca gttatttggt ggcagaccgg
     4141 accctgacct gttattcagg gaactgctca gtgacagtta cagctatttc tacggggcca
     4201 gccagtcggc cagtcagatt atgcgtaaga atgtgacaat aaatgcactg aaagagggta
     4261 tcaccagtaa tgcggcccgt aacggtgata cggccagcct ggtcagtctg gccaccacct
     4321 catcaatgga gaaacagaga ctggcgcacg tctctgtcgg acatgtaata atgcgaaacc
     4381 tgccgatggt ccagaccatt ctgaccggaa taaccattgg tattttcccg cttctggtac
     4441 tggctgctgc atttaataaa atgacactgc cggtgctgaa gggctatgtg tttgccctta
     4501 tgtggctgca gacatggcct ctgctgtacg ccattctgaa cagtgccatg acattctatg
     4561 cgaagcagaa cggtgccccg gtggtgttgt cggaagtctc tcagatacag ctgaaatact
     4621 ccgacctggc atcaaccgca ggttatctct cagcgatgat cccccctctt tcgtggatga
     4681 tggtacgagg ccttggtgcc ggattttcca gcgtatacag ccactttgcg tcttcttcca
     4741 tcacgccaac cgccagtgcg gcaggcagtg tggttgacgg taattactcc tacggcaaca
     4801 tgcagacgga aaacgtgaac ggcttcagct ggagcaccaa cagcaccacg tcgtttggtc
     4861 agatgatgta ccagactggc agtggcgcaa ccgccacaca gacccgtgac ggcagtatgg
     4921 tgatggacgc cagtggggca atgtcccgtt tgccggttgg tattaatgca acgcgtcaga
     4981 ttgcagcagc gcaacaggaa atggcccggg agtcgatgaa taaggcggaa agtgccctgc
     5041 atggtttcag cagcagtatt gccagtgcct ggaacacact cagccagttt ggctctaacc
     5101 ggggaagcag tgattcagtc accagtggcg ctgacagcac gatgagcgcg caggactcca
     5161 tgatggccag ccgtatgcgc agcgcagtgg aaagctatgc gaaggcgcat aatatcagtg
     5221 atgagcaggc gactcaggag ctggcatcca gaagtacgag agcttctgcc gggatgtatg
     5281 gagatgctca tgctgaatgg gggattaaac ctaaaattct tggtgttggt ggtggcgcag
     5341 gtgtcagagg tggtggtcgg gcaggtattg actggagcga tgatgatgcg catcaggcca
     5401 gcagtggttc acgggccagt catgatgccc gtcatgatat cgatgccagg gctacacaag
     5461 actttaaaga ggcaagcgat tactttacca gtcgcaaggt cagtgaatcc ggcagccata
     5521 ctgacaataa tgccgattcc agggtggacc aactgtctgc agccctgaac tcagcgaaac
     5581 agagttacga ccagtacacg acgaatatga cccgcagcca tgaatatgct gaaatggcct
     5641 cccgtactga aagcatgagt gggcagatga gtgaagacct gtcacaacag tttgcacagt
     5701 atgtgatgaa acacgcgccg caggatgcag aagctattct gacaaacacc agttcaccgg
     5761 agattgcaga acgtcgtcgg gcaatggcct ggtcttttgt tcaggaacag gtacagcctg
     5821 gtgttgataa cgcctggcgt gaatcccgtg gcgatatcgg taaaggaatg gagagcgtac
     5881 cttcgggcgg tggcagccag gatattattg cagaccatca ggggcatcag ggtattattg
     5941 agcaaagaac gcaggacagt aatatccgta atgatgtaaa acatcaggtt gataatatgg
     6001 tcacagaata ccgtggcaat atcggagata ctcagaacag catcctcggc gaggaaaata
     6061 ttgtaagagg gcagtattct gaactgcaaa atcatcataa aacagaggca ttatctcaga
     6121 ataataaata taatgaagaa aagtcggctc aggaaagaat gcctggtgct gacagcccac
     6181 aggaactgat gaaaagagca aaggagtatc aggataagca caagcaataa tattgtatgg
     6241 gggagatgat gatgaaaaat tcagctcata ttgctctgat aacggtgata cagctcattg
     6301 cctgttatct ggctgattgg gggagcgctg aaacagtttt tatcctgtgc tttattgtac
     6361 tatggcaggg attgtttata tggttgtttt ctcaaatacg aaaaaaacgg catgtgtcgg
     6421 atgagtttaa gtttagtaca ggcgtttggt atataacgat gccagtatct tccttactct
     6481 ctccgttatt aagcctgatg gtttttatca ttggtacact atacgaatta aggcgagtta
     6541 gtggttgtgt aagtgtaaga gaatggggcg ggaatcaact taaaaaccaa tatgatgggg
     6601 gtgaaaagtt agagttcggg ttcgttgaat acaccccaac tacctactat aacccttcaa
     6661 ccggatatcc tatgcatgga ggatttgatt cagcaggaaa tacttttggg actcgttggc
     6721 aggactatga tgataggcga taagaaaact gtagcttgta ataaaggtaa tttgatgaaa
     6781 acaaaaaaat tgatgatggt tgcactggtc agttccactc tggccctttc agggtgtggt
     6841 gcgatgagca cagcaatcaa gaagcgtaat cttgaagtca aaactcagat gagtgaaacc
     6901 atctggctgg agccttccag tgaacgcacc gttttcctgc aaatcaaaaa cacatctgac
     6961 aaggatatga gcggattgca ggcaaaaatc agtgatgccg ttaaagccaa aggctaccag
     7021 gttgtgacct cacctgacaa ggcatactac tggatccagg ccaatgtcct gaaggcagac
     7081 aaaatggatt tgcgggagtc tcagagctgg ctgaacagcg gttatgaagg tgccattgcc
     7141 ggtgcagcat tgggcaccgg catcacagga tataactcca gctctgccgg tgcggcgctg
     7201 ggcgtaggga ttgccacagg gttgattggt atggctgccg atgcaatggt ggaggatatc
     7261 aactacacca tgatcacgga tgtgcagatt gcagagcgga caacatcgaa agtcagaact
     7321 gataatgttg cagctctccg tcagggaacc tccggtgcaa aagtgcagac cagcacatca
     7381 accggaaatc aacataaata ccagacccgt gtggtatcca atgccaacaa ggttaacctg
     7441 aaatttgaag aagcgaagcc agtactggaa gaccagctgg ctaaatccat cgcgaacatc
     7501 ctgtaaatta cccatccggc ccggacagat tgtaccgggc cgatattcct gccccggatg
     7561 aatacatgaa ccgtgcagaa tctcgtctct cgcaggtact ctttctactg ggctatgaca
     7621 gtgaaaagta tcctgagcat gtatgtcggg agttattaaa agaactttac cggtttgctg
     7681 atgcaaccgg agattctgaa ctggccatag cctgccagag atacctgaca gcactggaac
     7741 cgttctctgc atatgagatc ggcgtaagcg caacagaata tcttcctgtg agtgaatatc
     7801 cgaccggacg tcgcagaaga aaatagctga cgcctcgcgt tgctcgttgt ccaaccccgg
     7861 aaagtaacaa aataaatttt gtcacttccc ggcgtttccg aaataaatat gagaacactg
     7921 aacggttgtg caaattcttt gtgcgaccgt gaagtgttgc cggcgaagcc ggaagcttcc
     7981 ggagaaagtc attatgacat cagacaatat cgctgaatat gttggtgaga ataatcaatt
     8041 tactgagaag cctgtaactg aatacccccc ggtgatacag gataatacag cgaccactaa
     8101 agactctctg tacagcaata atattgacac tgacctttta gtggttatcg gagggcttgt
     8161 tgttatattg tttctcatga tgcttgtcat gtactttatt aattctctca ggcataagga
     8221 attaactgaa atactgacaa aaatatcttt tcctgagaaa tccggtagca atccgcttaa
     8281 gccggaagag ggccgctgta atttgttgtc cctgtgtgtg ctggccccgg tattctgtac
     8341 ccccaaaaag ctggcagaag tctccagaca gatcatcgtg accagtgcgc aggaaattgc
     8401 ctggacgccc gggagtagcc gtattgatgg tgagcaggaa gaaaatatta cctggtctct
     8461 gtcgtcacag actcatgccg ataaatacga tctcatcaat aaaacgtccc gtgtttctcc
     8521 tgacagtggc cacttttatg aaccgggcac ggctctggat tatcgtgaac gggtacgcct
     8581 ttttattcag atccagtttt cacacgagag tgactggtct gaaatattgc gcctgactga
     8641 tatcgtgctg gaaaaagttt cttccggcag gtcagcaggc ggctatcacc accggtatac
     8701 agggtataca tggtttgttc tgtgaatgta ttccacaccg ctcttttctg ctctgtacct
     8761 gactttaccg gagtttcatc atgagtttta acgcaaagga tatgacccag ggcggtcaga
     8821 ttgcgtccat gcgtatccgc atgttcagcc agatcgccaa tatcatgctt tactgcctgt
     8881 ttattttttt ctggatactc gttggtctgg ttttatgggt aaaaataagc tggcagacgt
     8941 ttgtgaacgg ctgtatttac tggtggtgta ccacgctgga aggcatgcgg gatttaatca
     9001 ggtcccagcc ggtctatgag atccagtatt acggcaaaac cttccggatg aacgcggctc
     9061 aggtactgca tgataaatac atgatctggt gcggtgagca gctatggtct gcattcgttc
     9121 tggcgtctgt cgtggccctg gttatctgcc tgatcacctt ctttgttgtc tcctggattc
     9181 tggggcgtca gggtaaacag cagagcgaaa atgaagtcac aggtggtcgt cagctgactg
     9241 acaatccgaa agacgttgcc cggatgctga aaaaagacgg caaggactcc gatatccgga
     9301 ttggcgacct gccgattatc cgggattctg aaatccagaa cttttgtctg cacggaacag
     9361 tatcgacggg taagtcagag gttatccgac gtcttgccaa ctatgcccgt aaacgtggtg
     9421 atatggtggt gatttatgac cgttcgtgtg agtttgtcaa aagttactat gatccctcca
     9481 ttgataagat cctgaatccg ctggatgcac gctgtgccgc ctgggattta tggaaggagt
     9541 gtctgacaca gccggatttt gataatgtcg cgaacacgct gatcccgatg ggcacaaaag
     9601 aggacccgtt ctggcagggc tccgggcgca ccatctttgc ggaagcggcg tacctgatgc
     9661 gtaatgaccc caaccgcagc tacagcaaac tggtggatac actgctttcc atcaaaattg
     9721 aaaaactgcg tacctatctg cgtaactcac cggcggctaa cctagtggaa gagaaaatcg
     9781 agaaaacggc gatttccatc cgtgctgtgc tgacaaacta cgtgaaagcc atccgttacc
     9841 tgcaggggat tgagcacagc ggtgagtcct tcactattcg tgactggatg cgtggtgtcc
     9901 gggaagataa gcaaaacggc tggttgttta tctcgtcgaa tgccgacacc catgcctccc
     9961 tgaagccggt ggtttccatg tggctgtcca ttgccattcg tggtctgctg gcaatgggag
    10021 aaaaccgtaa ccgtcgtgtg tggtttttct gtgatgagtt acccacgctg cataaactgc
    10081 cggatctggt ggagatcctg ccggaagccc gtaagttcgg tggctgttat gtgtttggta
    10141 ttcagtccta tgcccagctg gaagatatct acggtgagaa agcggcggcc acgctgtttg
    10201 atgtgctgaa tacccgagcc tttttccgct ctccgagtca taagattgca gagttcgctg
    10261 ccggtgaaat tggtgaaaaa gagcacctga aagccagcga gcagtattcc tacggtgcag
    10321 acccggtacg tgacggggta tcaaccggta aggagatgga gcgccagacg ctggtcagtt
    10381 attccgacat tcagtctctg ccggatctga cctgttatgt caccctgccc ggaccgtatc
    10441 cggcagtaaa actctccctg aaatatcagg cacgaccgaa ggttgctccg gagtttattc
    10501 cgcgtgacat caatccggaa atggagaacc gtctgagtgc cgtacttgct gcacgggaag
    10561 cagaagggcg ccagatggcc agcctcttcg aaccggaggt tgcctccgga gaagacgtga
    10621 ctcaggct
//
LOCUS       DAZGSP010000149        10575 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZGSP010000149 DAZGSP010000000
VERSION     DAZGSP010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.150"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            168..1535
                     /gene="dcuC"
                     /locus_tag="ACHK74_004611"
     CDS             168..1535
                     /gene="dcuC"
                     /locus_tag="ACHK74_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFP0223314.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1578..2075)
                     /gene="sspB"
                     /locus_tag="ACHK74_004612"
     CDS             complement(1578..2075)
                     /gene="sspB"
                     /locus_tag="ACHK74_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFP0223315.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2081..2719)
                     /gene="sspA"
                     /locus_tag="ACHK74_004613"
     CDS             complement(2081..2719)
                     /gene="sspA"
                     /locus_tag="ACHK74_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFP0223316.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3114..3506)
                     /gene="rpsI"
                     /locus_tag="ACHK74_004614"
     CDS             complement(3114..3506)
                     /gene="rpsI"
                     /locus_tag="ACHK74_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP0223317.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3522..3950)
                     /gene="rplM"
                     /locus_tag="ACHK74_004615"
     CDS             complement(3522..3950)
                     /gene="rplM"
                     /locus_tag="ACHK74_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFP0223318.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4169..5296)
                     /gene="zapE"
                     /locus_tag="ACHK74_004616"
     CDS             complement(4169..5296)
                     /gene="zapE"
                     /locus_tag="ACHK74_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFP0223319.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5490..5888
                     /gene="zapG"
                     /locus_tag="ACHK74_004617"
     CDS             5490..5888
                     /gene="zapG"
                     /locus_tag="ACHK74_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFP0223320.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            6042..7409
                     /gene="degQ"
                     /locus_tag="ACHK74_004618"
     CDS             6042..7409
                     /gene="degQ"
                     /locus_tag="ACHK74_004618"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFP0223321.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7499..8566
                     /gene="degS"
                     /locus_tag="ACHK74_004619"
     CDS             7499..8566
                     /gene="degS"
                     /locus_tag="ACHK74_004619"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFP0223322.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8629..9567)
                     /gene="mdh"
                     /locus_tag="ACHK74_004620"
     CDS             complement(8629..9567)
                     /gene="mdh"
                     /locus_tag="ACHK74_004620"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP0223323.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            10002..10472
                     /gene="argR"
                     /locus_tag="ACHK74_004621"
     CDS             10002..10472
                     /gene="argR"
                     /locus_tag="ACHK74_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFP0223324.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2598 a         2681 c         2633 g         2663 t
ORIGIN      
        1 ttcttacaaa ataatcataa gcgcatattt tttaatgaaa aatcacctca cctacaatta
       61 aaaacacgac atccgcacca taaatagact tgcaaaaaat ataacatcat tgttttcaat
      121 ctgccgttta tgggattgac cgttttcttt tgacacggag ttcaacaatg ttcggcataa
      181 ttatatctgt catcgtatta attacgatgg gctatttgat cctgaaaaac tacaaacctc
      241 aggtggtgct ggctgccgca ggtatcttcc tgatgatgtg cggtgtctgg ttagggttcg
      301 gtggtgtact cgatcccgcc aaaagcagcg gctacttgat cgtcgatatt tataatgaaa
      361 tcctgcgcat gctgtccaac cgcattgccg gattggggct atcgattatg gcggtgggcg
      421 gttatgcccg ctacatggag cgcatagggg ccagtcgcgc gatggtgagc ttgttaagcc
      481 gcccgttaaa actcattcgc tcgccgtata ttattctgtc ggcaacttac gtcatcggtc
      541 aaatcatggc gcagtttatt accagcgcct ccggtctggg tatgttgctg atggtcacct
      601 tatttccgac actggtgagt ctgggagtaa gtcgtctctc tgcggtggcg gttatcgcaa
      661 ccacaatgtc cattgagtgg ggaattctgg aaacgaactc catttttgca gcccaggtcg
      721 cgggaatgaa aattgccact tacttcttcc actaccagct tccggtcgcc tcttgcgtca
      781 ttatctcggt ggcgatctcc cactttttcg tgcaacgcgc ttttgacaaa aaagataaaa
      841 atatcaatca cgaacaagca gagcaaaaag ctctcgataa tgtcccgccg ctctattacg
      901 ccattttacc tgtgatgccg ttaatcctga tgctcggctc gctgttcctc gcccacgtcg
      961 ggctgatgca gtcagaactg catctggtgg tggtgatgtt actgagtttg acggtgacga
     1021 tgtttgttga gttcttccgc aagcataact tgcgcgaaac aatggacgat gtgcaggcgt
     1081 tttttgacgg catgggtacg cagtttgcca acgtggtaac gctggtggtc gcgggtgaaa
     1141 tatttgcgaa aggcttaacg acgattggca ctgttgatgc ggttatcagg ggtgcggagc
     1201 attctggtct gggcggtatt ggcgtgatga ttattatggc gctagtcatt gccatttgtg
     1261 ccattgtgat gggctctggc aatgcgccgt ttatgtcatt tgccagtctt attccgaata
     1321 tcgcagccgg actacatgta ccagcggttg taatgattat gccgatgcat tttgccacga
     1381 cgctagcgcg cgcggtttcg ccgattactg cggtggtggt cgttacgtca ggaattgcag
     1441 gcgtttcgcc ttttgcggtg gtgaagcgaa cagcgatccc catggcagtc ggtttcgtgg
     1501 tgaatatgat tgccacaatc acgctatttt attaagccat taaaagacaa aacaggccac
     1561 cagggcctgt ttttgtatta cttcacaacg cgtaatgccg gtcgaccacc gcgtggtggc
     1621 tgcggaggtt catcgtcagg atgagtgtca tcatcgtgat ctggcttgtc gccatcaata
     1681 accgacataa cggtttcgtt gtctgccgat gcctcttcat cattcatgat gctggtatct
     1741 tcatcgtagg cagcttcagg ctcaaacatt gtgcctgcgc cattttcacg ggcgtagata
     1801 gccagcacgg cagccagcgg cacagaaacc tgacgcggaa tgccgccaaa gcgcgcgtta
     1861 aagcgcacct catcattcgc cagttccaga ttgccgacag cacgcggcgc aatgttgagt
     1921 acgatttgtc cgtcacgcgc atattccata ggaacctgca cgccagggag cgtcacatcc
     1981 accaccaggt gcggcgtgag ctggttatcc agcaaccact catagaatgc acgcagcaga
     2041 tagggacgac gtggtgttag ctgtgacaaa tccatacaga ttaactccgg cccagacgca
     2101 tttcacgttc tgcttcagtt aaagaagcaa ggaaagagtc acgctcaaag acgcgggtca
     2161 tatagccttt cagctctttc gcacccgggc cgctgaactc gatgcccagt tgcggcagac
     2221 gccacagcag cggagcaaga tagcaatcga ctaggctgaa ctcatcgctc aggaagtacg
     2281 gcttctgacc gaagaccggc gcaatcgcca gcagttcttc gcgcagttgc ttacgtgcgg
     2341 catctgcttc agaagctgaa ccgttgatga tggtgttcat cagcgtgtac cagtcttttt
     2401 cgatacgatg catgtacaga cggctttcac cgcgagctac cgggtaaaca ggcatcagtg
     2461 gcggatgcgg gaaacgctca tccagatatt ccataatgat gcgagattcc cacagggtca
     2521 gctcacgatc caccagggtc ggaacgctct gattcgggtt gaggtcaatc agatcctgag
     2581 gcggattgtc cttttccacg tgttcgatct cgaaacttac acctttctca gccagcacaa
     2641 tgcggacctg atggctatag atgtcagtag gaccggaaaa cagcgtcatt accgaacgtt
     2701 tgttggcagc gacagccatg aaaacctcca ggtatagtca gaatttttac tgctaccagc
     2761 caccaggtgg ccagtcagaa gttgtgttac ccagtaagga acgactctct ttgttcgaaa
     2821 atcaaacaaa aaatgagcaa tacccgacat ttgggcagaa aattggatga tagtttacca
     2881 gattttgcga cctttgtggt gagtcgatgc cggaaatggg gaaaaagaga tgcgctttag
     2941 tctgaaatag ttgacttagt cccttattgg cgatgtggtt tttgttttgc ctgtctgtca
     3001 gacggtagca aaaagcaact ttccaatttt tacgctgatt cagaatttaa ccataaaaaa
     3061 acccgccgaa gcgggttttt tcgcaaattg ttttctgccg gagcagaagc caattaacgt
     3121 ttggagaact gcggacgacg acgtgcttta cgcagaccga ctttcttacg ttcaacctga
     3181 cgagcgtcac gagtaacgaa gccagcttta cgcagttcag aacgcaggga ctcgtcgtat
     3241 tccatcagag cgcgggtgat accgtgacgg atcgcaccag cctgaccaga gataccacca
     3301 cctttaacgg tgatgtacag gtccagtttc tcaaccatgt cgaccagttc cagcggctga
     3361 cgaactacca tgcgagcagt ttcacgaccg aagtactgtt ccagagaacg ttggttgatt
     3421 acgattttac cgttgcccgg tttgatgaaa acgcgagctg cggaactttt gcggcgacca
     3481 gtgccgtagt attgattttc agccattgcc tataatcccg attagatgtc aagaacttgc
     3541 ggttgctgtg ccgcgtggtt gtgctcgtta cccgcgtaaa ctttcagttt acggaacata
     3601 gcacgaccca gcgggccttt tggcaacatg cctttaaccg cgatttcaat cacacgctca
     3661 ggacggcgag caatcatctc ttcaaaggtc gcttgtttga taccaccgat gtggccggtg
     3721 tggtgatagt acactttgtc agtacgcttg ttgccggtta cagcaacttt gtcagcgttc
     3781 agaacgatga tgtaatcacc ggtatctacg tgcggagtgt attccgcttt gtgcttaccg
     3841 cgcaggcgac gagccagttc agtagccaga cggcccagag ttttaccggt cgcgtcaaca
     3901 acataccagt cgcgttttac ggtttctggt ttagctgtaa aagttttcat taaaagctta
     3961 cccaataaat agttacacgt tggtgaacac ccaaacgtct tcaattgttg aggttcacac
     4021 gacaaagtcc ggcaaaccta ccccttcgaa tagcctatgc cagcacacaa aaagttttgg
     4081 gaaaaaaact ttcttgtaac gtggggtcgc aggattatag agaagtcggg gtcaaagatc
     4141 gacccctttt tgtgatttgt gacaggtttt aacccgccaa atgctcgcgc ttcagatact
     4201 cttcgctttg catctcttgc agacgtgaca ggcaacgctg gaactcaaac ttcagccgat
     4261 cgccctgata aatttcatac agcggcactt ctgcactcac cactaatttg acatggcgct
     4321 cgtaaaactc atccaccagc gcaataaagc gccgcgcttc gctctccatc aaccgcgtca
     4381 taactggtac atcaaacaac atgaccgtat gaaagagacg tgagagcgca atatagtcat
     4441 gctgactgcg ggcgtcgacg cacagcgtag taaaagagac cgccagcgtc tggttctcga
     4501 cgcccattgt cgccaatggc cgatggttga tttctaacgt cggtgaattt tctcgtttcg
     4561 cccccgccag cgccaaccat agtttatcca tctgcgcccg ggtttcatcg ttgagtggcg
     4621 aaagccacag atgcgcctga gtgagtgtac gcagtcgata atcaacacca gcgtccacgt
     4681 tcattacatc acaatgctgt ttaatggcat cgattgcagg cagaaaacgc gcacgttgca
     4741 ggccatttcg ataaagttca tccggcggaa tatttgacgt cgctaccagg gtaatgccgc
     4801 gggcgaacag ggctttcatc agaccgccaa gtagcatggc gtcggtaata tcagaaacaa
     4861 aaaattcgtc aaaacagagc acgtcagttt cggctttaaa gcgatcggca ataatttcca
     4921 gcggatcggt ctgcccctgt aaggcagtta gctcttcgtg cacccgcagc ataaaacggt
     4981 gaaagtgcag gcgctgtttc cgctctcccg gcaggctttg atagaaaagg tccatcagcc
     5041 aggttttccc gcgtcctaca ccgccccaca tatataagcc acgcactggc gtatgctttg
     5101 tgtcttcgcg tttaccccac agcttaccga cccgcgccat tagcccactc gtcctggggg
     5161 ctggtggcct gctattgatg agttcttgat aaataatttc caggcggctg acggcctctt
     5221 tttgaacgtc gtcgggttga tggctgcctt cattgagcgc cttcaggtat tgcgatgttg
     5281 gggtaacgct ttgcatgatc ttattgttat tccttgaata atcggtgcgc cgttgttcac
     5341 ggttgacgaa aaaaaggccg ttctacacta cgcgatatgc agtcgggatt ccacttctgt
     5401 ggaattaacg gttatagtgg cataatcagc cgcaggcatg gagcctgaag ccaacaccct
     5461 acggaaacaa aagacaacgg gagatgttca tgacctggga atatgcgcta attgggttag
     5521 tcgtcggcat cattattggt gctgtggcca tgcgttttgg taatcgtaaa ctacgccagc
     5581 aacaggcgtt gcagtacgaa ctggaaaaga ataaagctga actggacgag tatcgcgaag
     5641 agctggttag ccactttgcc cgcagcgcgg aattactgga taccatggcg cacgactatc
     5701 gccagctgta tcagcacatg gcgaaaagct ccagcagcct gctgccggaa ctgtctgctg
     5761 aagcaaaccc gttccgtaat cgtctggcag agtctgaagc cagcaacgat caggcaccgg
     5821 tgcagatgcc tcgcgactat tctgaaggtg catccggcct gctgcgtact ggcgcgaagc
     5881 gcgactaatt tatttttcgg gcgcagccat tgcgccctcc tcttctctcc ctccccgact
     5941 atcatttaat ctggtgtctc attgttagcc gtctgaaaat tcaataacat caaactgttt
     6001 tgaatctctt ttcttatcat tcaggtacga gagcaggaat aatgaaaaaa caaacccagc
     6061 tgttgagtgc attagcgtta agtgtcgggt taactctctc ggcgtcattt caggccgtcg
     6121 cgtcgattcc aggccaggtt gccgatcagg cccctctccc cagtctggct ccaatgctgg
     6181 aaaaagtgct tccggcagtg gtgagcgtac gggtggaagg aacggccagt cagggacaga
     6241 aaatcccgga agaattcaaa aagttttttg gtgatgattt accggatcaa cctgcacaac
     6301 ccttcgaagg tttaggttcc ggtgtcatca tcaacgccag taaaggctat gtgctgacca
     6361 acaaccatgt gattaatcag gcacagaaaa tcagtattca gctcaatgat gggcgcgagt
     6421 ttgatgcaaa actgattggt agcgatgacc agagcgatat cgccctgtta caaattcaaa
     6481 acccgagcaa attaacgcaa atcgctattg ccgactccga taaattgcgc gtcggtgatt
     6541 ttgccgtagc ggtcggtaac ccatttggcc ttgggcaaac cgccacctct ggcattgttt
     6601 ccgcattagg ccgcagcggg ttgaatcttg aaggtctgga aaactttatc cagacagatg
     6661 cttccattaa ccgcggtaac tccggcggtg cactattaaa ccttaacggt gagttaattg
     6721 gcatcaacac tgcaatcctt gcgcctggcg gcgggagcgt cgggattgga tttgccatcc
     6781 ccagtaatat ggcgcgaaca ctggcgcagc agcttatcga ctttggtgaa atcaaacgcg
     6841 gtttgttagg catcaaaggc accgagatga gtgccgatat cgccaaagcc ttcaaccttg
     6901 acgtgcagcg tggcgcgttt gtcagcgaag tgttgccagg ttctggctcg gcaaaagcgg
     6961 gcgtcaaagc gggcgatatt attaccagcc tcaacggcaa accgctgaat agctttgctg
     7021 agttgcgctc tcgtatcgcg accaccgagc cgggcacgaa agtgaagctt ggcctgctgc
     7081 gtaacggcaa gccactggaa gtagaagtga cgctcgatac cagcacctct tcgtcggcca
     7141 gcgctgaaat gatcacgcca gcgctggaag gtgcaacgtt gagcgatggt cagctaaaag
     7201 atggcggcaa aggtattaag atcgatgaag ttgtcaaagg aagcccagct gctcaggctg
     7261 gcttgcaaaa agacgatgtg atcattggcg tcaaccgcga tcgggtgaac tcgattgctg
     7321 aaatgcgtaa agtgctggcg gcaaaaccgg ccatcatcgc cctgcaaatt gtacgcggca
     7381 atgaaagcat ctatctgctg atgcgttaat gtcgtaaacc gggcatcagg cttacgtgtg
     7441 atgtccggtt aactcgtggt atgctgctgc cgttcccttt tttaatgacg cctccatcat
     7501 gtttgtgaag ctcttacgtt ccgttgcgat tggattaatt gtcggcgcta ttctgctggt
     7561 tgccatgcct tcgctgcgca gccttaaccc gctttccact ccgcaatttg acagtaccga
     7621 tgagacgcct gccagttata atctggcggt tcgccgcgcc gcgccagcgg tggttaacgt
     7681 ttacaaccgt ggtttgaaca ccaactctca caaccagctt gagatccgca ccctgggatc
     7741 cggtgtaatc atggatcaac gcggttatat catcaccaat aaacacgtca tcaacgacgc
     7801 cgatcagatc atcgtcgcct tacaggatgg acgtgtattt gaagcattgc tggtgggatc
     7861 tgactctcta accgatctgg cggtacttaa aattaatgcc actggcggtt tacctaccat
     7921 tccgatcaat gcacgtcgcg taccgcacat tggcgacgtg gtactggcga tcggtaaccc
     7981 gtacaacctc gggcagacca ttacccaggg gattattagt gctacgggtc gaatcggtct
     8041 gaacccgacc gggcggcaaa acttcctgca aaccgatgct tccattaacc acggtaactc
     8101 tggcggcgcg ctggtgaact cgctgggaga actgatgggc attaacacgc tgtcgtttga
     8161 taagagtaac gatggcgaaa cgccggaagg tatcggcttt gcgattcctt tccagttagc
     8221 aaccaaaatt atggataagc tgatccgcga tggtcgcgtg atccgcggct acattggtat
     8281 cggcggacgc gagatcgcac cactgcacgc gcagggcggt ggtatagatc aactgcaagg
     8341 gatcgtggtt aatgaagtgt cacctgacgg cccggcggcg aatgcgggta ttcaggtcaa
     8401 cgatctgatt atttcggtgg ataacaaacc ggccatctct gctctggaga cgatggatca
     8461 ggtggcagaa attcgccctg gttcggtgat cccggttgta gtgatgcgtg atgataagca
     8521 gttaacgctg caggtcacca ttcaggaata tccggcaacc aattaagtcg agtgctcaaa
     8581 acaaaaaacc ggagtctgtg ctccggtttt ttattattcg ctaattaatt acttattaac
     8641 gaactcttcg cccagggcga tatctttctt cagcgtatcc agcatacctt ccagtgcgct
     8701 ctgttcaaat gcgctcaggg taccgataga tttacgctct tccacgccgt ttttacccag
     8761 cagcagcggt tgagagaaga aacgggcgta ctgaccgtcg ccttcaacgt aggcacattc
     8821 gacaacgcct tgttcgccct gcagtgcacg aaccagagac agaccaaaac gtgcagctgc
     8881 ctggcccata gacagggttg cagacccacc accggctttc gcttcaacca cttcagtacc
     8941 tgcgttctgg atacgtttgg tcagatcagc cacttcctgc tcggtaaaac taacgccagg
     9001 aacctgtgac agcagcggca gaatggtaac accagagtga ccgccaataa ccggcacttc
     9061 aacttcgcct ggctgtttgc ctttcagttc cgcaacaaaa gtgttggaac gaatgatatc
     9121 cagcgtggta acgccgaaca gtttgttttt gtcataaaca ccggcttttt tcagcacttc
     9181 agcagcaatc gcaactgtgg tgttaaccgg gttagtgata ataccaatgc acgctttcgg
     9241 gcaggttttc gcaacttgct gtaccaggtt tttcacgatg ccggcgttaa cgttaaacag
     9301 gtcggaacga tccatacccg gtttacgcgc tacacctgca gagataagaa cgacatctgc
     9361 gccttccagc gccggagtcg catcttcacc agaaaaacct ttgattttca cagcagtagg
     9421 gatatggctc agatcgacag ccacaccggg agtcactgga gcgatatcat acagagagag
     9481 ttctgaacct gaaggcagtt gggtttttaa cagtagtgca agcgcctggc caataccgcc
     9541 agcagcgccg aggactgcga ctttcatcct aaactcctta ttatattgat aaactaagat
     9601 atgttgctcc gctgccgcga ccttaatcca caaaattgcc gtttacaatg accacatctc
     9661 aagaatgtgt agtcacgcaa gtttagcgtt tatgcattta attgccgtaa tcaggaacct
     9721 aacttacgta attaacagtc gcttatgcat tagcgcaaca ttccaacagg tggtgacaat
     9781 ataccctacc gttcagccaa aacaacatca atttgataac aattaattta cttttaagca
     9841 gaatttgcat gccgtgacgc aggcatgttt ctcaataacg aaatttgata aaatcccgct
     9901 ctttcataac attatttcag ccttcttcag ggctgactgt ttgcataaaa attcatctgt
     9961 atgcacaata atgttgtatc aaccaccata tcgggtgact tatgcgaagc tcggctaagc
    10021 aagaagaact agttaaagca tttaaagcat tacttaaaga agagaaattt agctcccagg
    10081 gcgaaatcgt cgccgcgttg caggagcaag gctttgacaa tattaatcag tctaaagtct
    10141 cgcggatgtt gaccaagttt ggtgctgtac gtacacgcaa tgccaaaatg gaaatggttt
    10201 actgcctgcc agctgaactg ggtgtaccaa ctacctccag tccattgaag aatctggtgc
    10261 tggatatcga ctacaacgat gcagttgtcg tgattcatac cagccctggc gcggcgcagt
    10321 taattgctcg cctgctggac tcactgggca aagcagaagg tattctgggc accatcgctg
    10381 gcgatgacac catctttact acccctgcta acggtttcac cgtcaaagac ctgtacgaag
    10441 cgattttaga gctgttcgac caggagcttt aatctctgcc ccgtcgcttc tggcggcggg
    10501 gaaaatgttg cttatccctc tcattcccct tctttctcct gcgatacatt taacgaatag
    10561 tgcgttttac cgcga
//
LOCUS       DAZGSP010000150        10337 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZGSP010000150 DAZGSP010000000
VERSION     DAZGSP010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.151"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..282
                     /gene="tssJ"
                     /locus_tag="ACHK74_004622"
     CDS             <1..282
                     /gene="tssJ"
                     /locus_tag="ACHK74_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFP0223325.1"
                     /translation="ADYQALFTGDSEILAGDIIAQKDVWMQPGGSVTVDMPLDDAAKF
                     TGVAAMFLEPDRKKNTWRVVLSRDELEPDTPRLIEVSGNTLTLLPVKDK"
     gene            285..716
                     /gene="tssE"
                     /locus_tag="ACHK74_004623"
     CDS             285..716
                     /gene="tssE"
                     /locus_tag="ACHK74_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP0223326.1"
                     /translation="MPRPSLYEILYGNFAGGLELNQVSAEEQVILSVLDNMQRILNTR
                     AGSLKHLPDYGLPDMTTVLQGMPGTAHQLMRVLSDVLLKYEPRIKRVNVTMQEQTQPG
                     ELHYVIDAELKDAGLVRYGTTFIPEGRVLLRHLKQQRYVQT"
     gene            974..1978
                     /locus_tag="ACHK74_004624"
     CDS             974..1978
                     /locus_tag="ACHK74_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP0223327.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARRISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTEPLQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPTGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQPGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFMMRLERQHGKLAEWVREQLNKKHSNVVACALANKLARIAWVITTQQKEYQA
                     "
     gene            2325..2627
                     /locus_tag="ACHK74_004625"
     CDS             2325..2627
                     /locus_tag="ACHK74_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223328.1"
                     /translation="MSHCTSDGRFVCNDGSLSQSKRFCSGYGVAPARRQVKSPVATKP
                     VQKKKSAAVRKVSQPLVAEKNEVVPVTEPRKPTCAPLYMASKPGFTHLPICSGNQY"
     gene            2824..4025
                     /locus_tag="ACHK74_004626"
                     /pseudo
     CDS             2824..4025
                     /locus_tag="ACHK74_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            4260..6005
                     /locus_tag="ACHK74_004627"
     CDS             4260..6005
                     /locus_tag="ACHK74_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP0223329.1"
                     /translation="MTPGERRVASRLESFLNDDCLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVSTLRQVNQEQVTLETDGLLKSEINPLVQVRRYACDTVNALPANPCL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKTLAGNDENAVEKIFPSAQTICQDEMTQS
                     VLPEVFQQKIAGMFTTGFRTRVTPRMRDILRAHLFPEVTVKQNSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIEARGL
                     ASRVTVSHFHSWCASMVKRHGIKVTADRKEYPDKCFSALEEAVNSGKITRTGYDAVLV
                     DEGHDFDSRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNPKRILHFAYAFSREYFEKHQNREIPLVQPQAGGEEGTEPEILRCASERDE
                     ARQVADWLEKRHALCGHWSDMAVLCPTERSAQQLQDVMTLRGVPFATCFDREGKKTYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPVLYVAFTRATRELLVT
                     CYRENSISRHLEDFA"
     gene            6071..6376
                     /locus_tag="ACHK74_004628"
     CDS             6071..6376
                     /locus_tag="ACHK74_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223330.1"
                     /translation="MNPLFNDIQMRLFYLNHSPYSWHWNVRFRPQEAVYIGSDTCHIT
                     ITCNQSGFHLTRDGQRLFTERYIRNLNELLPVLKRRWDVTPAIIRAVEYLSRVPVLH"
     gene            6431..6745
                     /locus_tag="ACHK74_004629"
                     /pseudo
     CDS             6431..6745
                     /locus_tag="ACHK74_004629"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            7070..7219
                     /locus_tag="ACHK74_004630"
                     /pseudo
     CDS             7070..7219
                     /locus_tag="ACHK74_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7394..7828
                     /locus_tag="ACHK74_004631"
                     /pseudo
     CDS             7394..7828
                     /locus_tag="ACHK74_004631"
                     /inference="COORDINATES: protein motif:HMM:NF023902.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            7971..8060
                     /locus_tag="ACHK74_004632"
                     /pseudo
     CDS             7971..8060
                     /locus_tag="ACHK74_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8465..>10337
                     /locus_tag="ACHK74_004633"
     CDS             8465..>10337
                     /locus_tag="ACHK74_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA helicase domain-containing protein"
                     /protein_id="HFP0223331.1"
                     /translation="MAKMIPAFGPIDNNSYGEKTVYTLLKEGLSDDFTVIHSLPWLSA
                     ATRNLGVKLPPSGEIDFLVLHPVYGVLALEVKSGIYRVEGTVFVHIKTKKHVDIVKQT
                     RNNVHGLARWLGGATTLRLRIGYGFIFPDSYFDDKIINAAMVDASVKPFRGIFVDKSQ
                     LPEMAKHTIDIMQYWKHALGNNELGDERVQLIIKSVCPEFDGTPDWGIRIIYDNKLWL
                     RLTHEQIKVIELLSEYQRVVVTGWPGTGKTLIGIEFARRLVKGQKKVLFITFNNLLSE
                     YIRQQTPESLCDVFTWHKLCHQARNKLSLSPESPAGWFESGCCEDLLNALNKNKLRDY
                     DALIIDEAQALHSSWLETLSCWFENKRIIAFCDETQTFSFEKGTNYADLQRIINPQFS
                     FNLSVVLRCPRAVTDYLIAVSPASYQITSPRNNEPDTLKELVVTDPLSTLLNEILQLK
                     ESSVLPDEITVLVPTDLVKNSLQNDMRTLGVNIETVSRFRGMESSVVITLYSESMEDA
                     QLFCAYSRATTAFIAIYDSEKLAWREKNKFLHKLVNRKEVQRAINEAKHNSSSSVLMA
                     GFFREGELKLSTVKLAWSKHFSSWLVEFDERNSPEEMWIDYLYSYLPYPIIYWFRDSC
                     "
BASE COUNT         2821 a         2180 c         2657 g         2679 t
ORIGIN      
        1 gcggattatc aggcgctttt tacgggggac agtgaaattc tggcaggcga tatcattgca
       61 cagaaagatg tatggatgca gccaggtggc tcagtaaccg tggatatgcc tctggatgat
      121 gcggcgaaat ttaccggtgt ggcagcaatg tttctggagc ctgaccggaa gaaaaatacc
      181 tggcgggtgg tgctgagccg ggatgagctt gagccggaca cgccccgtct tattgaagta
      241 tccggtaata ccctgacgct gttaccggtg aaggataaat agtgatgccg cgtccttccc
      301 tttatgaaat tctctatggc aatttcgctg gcgggctgga gttaaaccag gtcagtgcgg
      361 aagagcaggt tattctttcg gtgctcgata acatgcagcg tattctgaat acccgggccg
      421 gaagccttaa acatctgccg gattatggtc tgccggatat gacaaccgtt ctgcagggaa
      481 tgccgggaac ggcgcatcaa ctgatgcgtg ttctttcaga tgttctgctg aagtatgagc
      541 cacgtattaa gcgtgtcaac gtgacgatgc aggagcagac tcagcctggt gagttgcatt
      601 atgtcattga tgcagaactt aaagatgccg ggctggttcg ttacggaaca acatttatac
      661 cggagggcag ggtgttgttg cgccatctga aacagcagcg gtacgttcag acgtgagccg
      721 gagcagggag ggcagaacag aaataaacag tgatggatat atgtcaggtg tgaatgtgac
      781 attattccgg cttatgacaa cgctggcgga atgtttcatc atcctgataa ctctggcata
      841 tcagcaataa caatgtatga gtttttaaat gaagaaaatt atctgttttc tgttcgtggg
      901 gctaatgaga tggtcacccc caccttaaca gcagtacgct gttaaggtga atccctttaa
      961 cggagagacc atcatgcaga acatcacact tattggtatc gatctcggaa agcactcctt
     1021 ccacattcat tgtcaagaca agtctggtaa ggctcttctg cgtaaaaaat ttacccgtac
     1081 taaactgatg cagtttctgg cgacatgtcc gccctcagtt gttgtaatgg aagcctgtgc
     1141 gggtgcacac tttatggctc gtcgtatctc tgattttggg catgaaacta agctaatttc
     1201 tccacagttc gttcgcccgt ttgtcaaaag taataagaat gattttgttg atgcagaagc
     1261 catatgcgaa gcggcttcac gaccatcaat gcgttttgtc cagccaagaa ctgagcccct
     1321 gcaggccatg agggcgttac accgggtccg agaatcactc atcagagata aggtgaaaac
     1381 aaccaaccag atccacggtt tcttgctgga atttggaatc agcctgccga caggtgatgc
     1441 tgtaataaaa cggttgtctc tcgtgttggc tgaacacgaa atccccgaat atctgagccg
     1501 ccttttggtg aggttgcata cccactacct ttaccttgtt gagcaaatag cagagctgga
     1561 atcagaatta agtcagtcaa tcaacgctga tgataccgct cagcgcatta tgacaattcc
     1621 tggtgttggg ccgattactg ccagcttact atcgtcgcaa cctggtgacg ggaaacagtt
     1681 ttcatgcagc agagattttg cggcttccac agggctcgtt ccccggcagt atagcacggg
     1741 aggaaaatcg actctgttgg gtatcagtaa gcgaggcgat aaaaatttgc gccggttact
     1801 ggttcagtgt gcccgctcat tcatgatgcg gcttgaacgc cagcatggaa aacttgccga
     1861 atgggttcgg gagcaactca acaagaagca ttcgaatgtg gttgcctgcg ctctggcaaa
     1921 taaactggct cgaattgcct gggtaatcac tactcagcaa aaagaatatc aggcttgata
     1981 taaatgctga tataactcaa ttggcttatt gtaaacaata agatacaaag ttgcctgtct
     2041 ggttttgcga agactgatta ttgatgacat gaacggcaaa tcgacctgat taaaaaccta
     2101 ataaataaaa tggctcactg aagccgaggg gctttttagg attatcaggt gcgaaactca
     2161 tcggggcgca ggcatcctga cctacagaga cgccggatag atttaagcaa gcccaccatt
     2221 aattaaaaaa tcagtgttgc aaaaacgggg gcgaccatag attattttcg gttcagacag
     2281 atgctgcgcg gggaagaaaa ccctgctcgg gtgctaaagg tggtatttca cactgcacgt
     2341 cagacggacg ttttgtctgc aatgacggaa gtctgagtca gtcgaagcgc ttttgttccg
     2401 gttatggcgt ggcacccgcc agacgtcagg tgaaatcacc agtcgccact aagccggttc
     2461 agaagaaaaa atcagcggct gtccggaagg tgtcacaacc gttagtggct gagaaaaatg
     2521 aggttgtacc ggtcactgaa ccccgtaaac ccacctgtgc gccgctttat atggccagca
     2581 aacccggatt tacgcatctg cccatttgtt ctggtaatca gtattaatct gtcactgaaa
     2641 tagcagagga atctgtgctc aggctgcagt tcatcctgta cttgcgggca tcataaagtc
     2701 ggacaacctt ccggatataa atatttccgg agagcgttaa tctgagatat tcagttactg
     2761 agaaggaaaa taaaggagag tcatggcagg aataaaaaag ataataatgc agcgggattt
     2821 gtcctgattt tagtttgcgt tattctctgg gggatttatg tggcggtcag ggcgctgata
     2881 aatcttaatg agcgctttat tgatgcagta tcgagtccgg caggtattat tggtttgttt
     2941 tttggtttgc ttactgtatt tgcaatttta cttcgttttt ttatttaccg gagactcaga
     3001 aagaaaacag cagcctttga gcaggccgtt tcagagctgg ttcagagaga gcggggtttt
     3061 aatgaaactg tcaatgccac gatagcccgg ggaatacgac aggaaaagga gcaacttgcc
     3121 agacgccgtg aggaatttca taccgccaga cagaaggcca gccgtgcaat gcaacggatt
     3181 gttgattccg cctggaaatt taaggcgaag actctgctgg ctggtgtaac gattaataac
     3241 tggcaaagca aatatgacca gcttcgtaag gaaagagagg cttacgctgc tgtcagtgag
     3301 aaaatagcct ttcttaacct ggaagataat tctgactggg agagtgtcag gcaacagttt
     3361 ctggacaaag tggcattgct ggaaaagcgc aggaagagaa ggaatatcag gcagagctta
     3421 agcgtcagat acgtgaagaa aaagagcgtc aggatgaact tgaccggcgc cagcgggaag
     3481 cggaagagga agaacggcgt cttgctgagc agcagaaact gattgaggag gcgctgcgcg
     3541 cagcagaagg ggctcacagg gaggagttag agaaacaacg cctggagctg gaacagaaaa
     3601 ttcaggaagc gcatgcgcag tatgaacgtg ctaaatccat ggctcagctt acgaaacagg
     3661 ggcatgttta tatcatttcg aatattggtt cgtttggtga ggatgtcttt aaaattggca
     3721 tgacccgacg gctggaaccc atggacaggg tcaaagagtt aagtggtgct tctgttccgt
     3781 ttgattttga tgttcacgcc atgatttcct gtgatgatgc gcctgcgctg gaaaaaacac
     3841 ttcatgacag tctggaaaaa taccgcatta acaggattaa cctgcgtaaa gaatttttcc
     3901 gcgtgaagct tgagaaaatc attaatgaag tggagcgtca ccacgggcag atggaatatg
     3961 tggctgaccc ggcggcgctg cagtacctgc agagtctgga atacgcagaa aatgaagcag
     4021 cctgacagtc agtgttctga ttttaccccg gccggatata acagaaggga tatctgcaga
     4081 taccctgcag catggttcac agtattctgg tcatgttaat gattctgttt tttatttctg
     4141 aataaagcga cacccacgat ataacatccg tggatgtcgg tggtgattat tttttgttca
     4201 cagattaagg tgcgtttatg gcgaaactga taaatgtatc ggcctccact gaggagagaa
     4261 tgacgcctgg ggagaggagg gtggcttcac gactggagag ctttctgaat gatgactgcc
     4321 ttgtctggta cgatattccg gtgggacgga aaaaccggca tccggatttt gtcattattg
     4381 acccggagaa cggactggta tttctggagg tcaaggactg gacggtttcc acgctgcgcc
     4441 aggtaaatca ggagcaggtc acgctggaaa ccgacggtct tctgaaatca gaaatcaatc
     4501 cgctggtaca ggttcgtcgt tatgcctgtg atacggttaa tgccttacct gcaaacccgt
     4561 gcttacggca gaatgacggt cagtataagg gacggctgaa cctggcctgg gcgtatggtg
     4621 tggtgtttac ccgcattacc cgtcagcaac tgaagacact ggcagggaat gatgagaatg
     4681 cggtggaaaa aatttttcct tcagcacaga caatctgcca ggatgaaatg acgcagtctg
     4741 ttttacctga ggtattccag caaaaaattg ccgggatgtt tactacggga ttcagaaccc
     4801 gggtgacacc ccggatgaga gatattctgc gcgcccatct gtttcctgaa gtgaccgtga
     4861 aacagaactc acaaatcaaa atcatggata ttcagcagga gattctggcc agaaatattg
     4921 gtgacgggca tcgtgtcatt catggtgtgg ccggttccgg aaaaaccatg attcttttgt
     4981 tccgttgcct ttacctggct gaaaccacac ccggaaagat tctggtgttg tgctacaaca
     5041 ttaccctggc cagctatctg cgggaatgca ttgaagccag agggttggcg tcacgggtta
     5101 cggtatccca ttttcacagc tggtgtgcct caatggtaaa acgccacggt attaaggtga
     5161 cggccgacag aaaagaatat cctgataaat gcttcagtgc actggaagag gccgtgaaca
     5221 gcggaaaaat cacccgaacc ggatatgatg ctgtactggt ggatgagggg catgattttg
     5281 acagtcgctg gctggccctg attgcccgtc tgtttgataa cgcatcccgt tcactgctcc
     5341 tgatgtacga tgacgcccag tccatttacc ggcgggagcg ggccctgaac ttttctctgg
     5401 ccagtgtggg gattcaggca cagggacgca cttctgtcct gccggtcaat tatcgtaatc
     5461 cgaaacgcat tctgcacttt gcttatgcat tctcgcgcga gtattttgaa aaacatcaga
     5521 accgtgaaat accgctcgtt cagccacagg cgggcggtga agagggtacg gagccggaaa
     5581 tcctgcgttg tgcttctgaa cgtgatgaag ccagacaagt tgcagactgg ctggagaagc
     5641 gccatgccct gtgtggtcac tggagtgata tggctgtgct gtgtccgaca gagcgttcag
     5701 cgcaacaatt gcaggacgtt atgacactac gcggggtgcc ttttgccacc tgttttgaca
     5761 gggaaggcaa gaagacatac tcgcgacgta aggatgtggt gcatttactg acgtaccaga
     5821 gcagcaaggg gctggagttc ccgtatgtgg cggttatcaa tgcgtctttc gttcactctg
     5881 gtgcagcgga tgagtctgaa gtgattccgg tgctttatgt ggcgttcacc cgggcaaccc
     5941 gtgaattact ggtgacctgt taccgtgaaa acagtatcag tcgtcacctt gaggattttg
     6001 cataataccg gattatttct ttattccgga ctttcatcat actgttatta tcaggtttat
     6061 ttaattcgcc atgaacccgt tatttaatga tattcagatg cgtctttttt atctgaacca
     6121 ctctccgtac tcctggcact ggaatgtcag attcagaccc caggaagctg tctatattgg
     6181 cagcgatacc tgtcatatca ccattacctg taatcagtcc ggttttcatt taaccaggga
     6241 cggacaacgg ctgttcactg aacgttatat cagaaacctt aatgagctgt taccggttct
     6301 gaaacggcgg tgggatgtga caccggcaat catccgtgcc gtggaatacc tttcccgggt
     6361 cccggtatta cactgacatt cttttcgtca ctgttaatca tgctttaata cagtaacacc
     6421 gaatctgccg atgacttctg aagtgaaact gaaaaccggt ggtgacccca ggtcattacc
     6481 ggattatgct gccctgcgtg atgaaatcag caaactgacc cacccggcac gtccggatgt
     6541 ggactggcgt tacgtggaaa cgctctgcct gcgtctgtat gaacacaatg gcgtggaatt
     6601 acagacggca tcctggtaca ccatcgcccg gatgcataca acagggttaa gtggactgaa
     6661 tgaagggctg gctctgattg tggcgctgac ccggcatcac tggtcagtga tgtggccgct
     6721 gaatacccac gcccggctgg aaattattac cggtctgttc aaccgtctgc aaaaaacgct
     6781 tcgggccatg cctccggacg accgtgacaa cctgcccctg ctgtatcaga ccgaaacatt
     6841 cctgaaggca ctgagtgaca cacttgcccg gcacgaactt aaacagggca gtaaagtggc
     6901 tttaccggaa gccatggtga agggatatat cacccgcctg gaaaatcagt ctgtgcaggg
     6961 cgaagcgtcg tctccggtaa cgcttccggc tcaggcactc cggtctgatg ctccggatgt
     7021 gcaggaacat cagaccctgc cgcactcccg tctggtgtat gttgtttctg aaataaaagc
     7081 agagcccaca ccctcccttc agaaatcccc gcccatgttt ctgaagccgt ttttggctgg
     7141 tgtctgtgcg gcattgctgg ctgtcagcgt ggccattccg ggatggcagt ttctgacaca
     7201 gccatcaccg gcggagcagc aactcagggc gctgctgaca cccccaccgg atgtgctttc
     7261 ggcagaccag atagcacagc tcagcagaac acgctcactg ttaaatcata cgtcagagtg
     7321 gatacccctt tccggtcaac agataacgcg actgacagaa ttacccctgg catacaacct
     7381 gcagcgcagt gcgcagctgc tgcagcaact gagggtactt ttccccgata atccccgggt
     7441 acaggaaatg gtggataact ggcagaagag tgtaaggagc cgtgcgctcc cggaagaggc
     7501 gatgacgggc tggaacgaag gcatgacccg cctacagcag ctggctgagc gcctgaaccg
     7561 tctggatgaa cagcggggaa aatacatgac agtcagtgaa ctgaaaacag aggtgtttgg
     7621 catcatgcag gcttttaacc ggcatatccc ggcagaagag cagttacgtc gctataatga
     7681 agtccgcaac cagaatggca gtgaacaaca gcaaaaactg acgcaggatg ccctgacgga
     7741 actgttgaac cgctactggt ttctgagaca tggagatgca ggtaagaaga cgataaataa
     7801 taaacaggat aaccagatta atatctgaca gattttattg ccattattta tccagaagcg
     7861 atgatgccat taatgcaaaa tgacatctga attgatcaca gattggacca atctgtgatc
     7921 aaatagaaca gaatacgcag atgataaagc ggctttccct cgaagattca caacgtcatc
     7981 tggcagaata tgggtgagag ttgcaggctg atgcgtacaa agcttacgat gcgttgtatg
     8041 aaactggccg ggtgaaagat gtcatccgta cggtgcgcgc cagactaaac aggcatcatg
     8101 atggaaaagt cgggtatcat tcacaggcat atttgtcaga atggtagcgt cagcgggttt
     8161 tgattacacc agttttgaag ctcatacttc ctatgaggca ctgcagaaaa cgaaagtgtc
     8221 gtttaggcgc tgggggaggg gttaaaaaag taacggcaga agtcaccctg aagttgaggc
     8281 agtcaactac tgttagctgg ctgctgcatg agagtgtgga gggagattgt atatattggt
     8341 tagtcagagt ctgcttcgga ataagtatga gtaattttta aaatgggcgg aagttatttt
     8401 taattactgg gctatgtaac aaatgcagat tcctgtcaga aacatgttat aaggaaatag
     8461 taatatggcc aaaatgattc cggcatttgg acccattgat aataatagtt atggggaaaa
     8521 aacagtctat acactgttaa aagaaggtct gtccgatgat ttcacagtga ttcatagtct
     8581 cccgtggtta agtgcggcga cacgaaattt aggggtgaaa ttacctccga gtggagaaat
     8641 tgattttctt gttctccatc ctgtatatgg tgttttggcg ttagaggtaa agagtggaat
     8701 atatcgcgta gaaggtactg tttttgtaca tataaaaaca aaaaaacatg tagatatagt
     8761 gaagcaaaca cgtaacaatg tccatggttt agcccgttgg ttagggggag caacaacgtt
     8821 acggttgcgt attggttatg gttttatttt tcctgatagt tatttcgatg ataaaattat
     8881 aaatgcagca atggtagatg cgtctgttaa accattcagg ggaatatttg ttgataaaag
     8941 ccaactcccg gaaatggcaa agcatactat tgatataatg caatactgga aacatgcatt
     9001 gggaaataat gagttggggg atgaacgggt tcaactaata ataaaatctg tatgtcctga
     9061 gtttgatggt actcctgact ggggaatccg tattatttat gataataaat tatggttaag
     9121 gctcacacat gaacaaatca aggtcattga acttctgtca gaatatcaaa gagttgttgt
     9181 aaccgggtgg ccaggaacag gaaagaccct gattggaatt gagtttgcac ggcggcttgt
     9241 taaaggccaa aaaaaggttt tatttattac tttcaataac cttctttcag aatatattcg
     9301 gcaacaaact ccggagtcat tatgcgacgt ttttacctgg cacaaacttt gtcatcaggc
     9361 tcgaaacaaa ttatccctgt cccccgagtc tccggcaggc tggtttgaat caggatgctg
     9421 cgaagatttg cttaatgcat taaataaaaa taaactaaga gattatgatg ctttaatcat
     9481 tgatgaagca caggcgttac attcttcgtg gctggagact ttatcttgtt ggttcgaaaa
     9541 taaaaggatt atcgcgtttt gtgatgaaac tcaaacattt tcctttgaaa aaggaacaaa
     9601 ttacgctgat cttcagcgaa taataaatcc ccaattttct tttaacttat ctgttgtgtt
     9661 aagatgtcct agagccgtta ctgattattt aatagctgtc agccctgcct cataccaaat
     9721 tacttcccct aggaataacg aaccggatac gctaaaagaa ttagttgtta cagatccact
     9781 ctccacattg cttaatgaaa ttttacaatt gaaagaaagt tctgtactgc ctgatgagat
     9841 tactgtcctt gttcctacgg atttagtgaa aaattccctc cagaatgata tgagaacttt
     9901 gggggtgaat attgaaacag tatctcgttt tcgaggtatg gaatcatctg tcgtgattac
     9961 attgtattcc gaaagcatgg aggatgcaca gttattttgt gcttattcaa gagctacaac
    10021 tgcttttatt gcaatttatg attcggagaa attagcatgg agagaaaaaa ataaattttt
    10081 gcataaactg gttaacagaa aggaggttca gagagcaatt aatgaagcaa agcataattc
    10141 ttcctccagt gttcttatgg ccggtttttt ccgcgaagga gagttaaaac ttagtactgt
    10201 aaaattagca tggtcgaagc acttttcttc gtggcttgtt gaatttgatg aaagaaattc
    10261 tccagaagaa atgtggatag attatttgta ttcctatttg ccttacccga ttatatattg
    10321 gttcagagat tcatgta
//
LOCUS       DAZGSP010000151        10306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZGSP010000151 DAZGSP010000000
VERSION     DAZGSP010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.152"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..183)
                     /locus_tag="ACHK74_004634"
     CDS             complement(<1..183)
                     /locus_tag="ACHK74_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0223332.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSD"
     gene            complement(183..3209)
                     /locus_tag="ACHK74_004635"
     CDS             complement(183..3209)
                     /locus_tag="ACHK74_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0223333.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASVVAQNTAAAKKSASDAGTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSVAATSAGAAKTSETNAAASEQSA
                     ATSASTAITKASEAATSARDAAASKEAAKSSETNASLSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIVQLSSTTNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNALFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTTYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGLIPDMRSWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGNHTH
                     SVTGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHT
                     HSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            complement(3274..3873)
                     /locus_tag="ACHK74_004636"
     CDS             complement(3274..3873)
                     /locus_tag="ACHK74_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP0223334.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMVG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIMGVGYKF"
     gene            complement(3940..7338)
                     /locus_tag="ACHK74_004637"
     CDS             complement(3940..7338)
                     /locus_tag="ACHK74_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0223335.1"
                     /translation="MGKGGSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLNSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDLSGTVNGVTPPAVQHL
                     TVEVTADSGEYQVLACWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVESVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWAVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            complement(7399..7971)
                     /locus_tag="ACHK74_004638"
     CDS             complement(7399..7971)
                     /locus_tag="ACHK74_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0223336.1"
                     /translation="MKTGAEAIRALATQLPVFRQKLSDGWYQVRIAGRDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGIFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(7968..8711)
                     /locus_tag="ACHK74_004639"
     CDS             complement(7968..8711)
                     /locus_tag="ACHK74_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP0223337.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGICNDLAAASTFV"
     gene            complement(8717..9415)
                     /locus_tag="ACHK74_004640"
     CDS             complement(8717..9415)
                     /locus_tag="ACHK74_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0223338.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGLELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(9415..9744)
                     /locus_tag="ACHK74_004641"
     CDS             complement(9415..9744)
                     /locus_tag="ACHK74_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0223339.1"
                     /translation="MKTFRWKVKPGMDVASSPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(9741..>10306)
                     /locus_tag="ACHK74_004642"
     CDS             complement(9741..>10306)
                     /locus_tag="ACHK74_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0223340.1"
                     /translation="AAMLTGSEQNCRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGG
                     AVGGGASASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGV
                     GNLYRLMRGYATGGYVGTPGSLADSRSQASGTFEQNNHVVINNDGTNGQIGPQALKAV
                     YDVARKAAMDVVTGQMRDGGLFSGGGR"
BASE COUNT         2074 a         3130 c         2642 g         2460 t
ORIGIN      
        1 atcactgttg aaaacagcca caaagccagc aggaatatct ggtggtgcaa tatcggtact
       61 gtttgctggc agacctgtat gaggcggaat atatgcgtca ccttcaccaa taaattcatt
      121 agttccggcc agcagattat aaatttttat ggtccgtgct tgttcactca ttctgaatgc
      181 cattatgcaa gcctcacaat atagttaaat gcgatgtttt tgacagtgtt ttccgcgtta
      241 ccagcagcgt taacggtgat ggtgtgtcca tgtgagccaa tcgcaaccga gtgcgtatga
      301 gcaccaatac cgacagtatg tgcgtgtgca cctgcagatg cagcggtgcc gctgacacta
      361 tgagtgtgcg ctcctgcagc acttgtattc acactggccg cagacactac cttatgtact
      421 gacccatttt gtgaccactg gctgactgct gatgcgcctg tgacactatg agtatggttt
      481 ccggcacttg cggctgtacc gctcacactg tgcgtatgcg ccccggtgtt attcgtggat
      541 ttagtgccgt aatcaaacga cgatgtggtt ttcgtcccca aatccgtact ggatgcgctg
      601 gcgctgtggg tgtgcgattt aatgccgtcc tgttcctgag acaatacggc ccgaccactg
      661 gcaggtttgc ccttaatcgt ccagctacgc atatcaggga tcaggcctga cggataagcg
      721 gctgcaagtt tcgggtatgc agatttgtca aaagtctgcc cctgcatcag ggcataacca
      781 gacggaacgg tatctgatgg ccacgggatt ggtgcaccga ctggataaaa ctctgcagga
      841 ggatgagccg aggtgtaaag ctgcgcccac ggcgaccagt ttgcgtcggt cgtatcccgt
      901 cgtgaacgaa taaatgccgg agcatgagca ccgcttgtac cactccagcc gatgagtaac
      961 tcaccttcgc caacggctgt catccctttc aggtgaatga tatttccata cgttgttgga
     1021 tatccgttgt tatacacctc gtataactca agacctgctg ccccctgcgt attgtctgtc
     1081 agcgcggcta cccgaccttt tgaagccaga ttaactgatg atactgctgt tccacctgac
     1141 ggtagcgccc cgatctctga tgccgttggc ttatttctgg agttatagtc ccttcgccag
     1201 ccaggtgaat aatctgttcc gtgattaata taggtaaaca gggcgttggt tgttccgcca
     1261 ccgctggatg tggtcggagt ggtaatgcgg atcgtcatcg ctgactttat ccccattact
     1321 tcaatgacag ctccggcgag atgaatatta ccgcagccag tatcagtaat gattttatta
     1381 ttgccataag accaggaacc cttgcacatc cagtatggat ggttaaatgc tccctgagaa
     1441 tccagccact cgataaactg tgcagtcgtc cagtttcctg ttgttgtgct tactgaccca
     1501 ccgaaggcac ggcaggcacc aatatttttc gtaaaggtgt ctttgccagg gatatccgca
     1561 ccgttctgat ctttctgcag acgtttctca gcattgtcat tggctgcttt tactgccttt
     1621 ggcgttgccg ccagcgtttc agacgtgctg ttggtcgtgc tgcttagttg gactatccct
     1681 ttctgtgctg tcgttgcatc ctgtgctgtg tatttcccgt tagccaggtc atacgcggcc
     1741 ttaactgctt ttggcgttgc cgccagtgac tcggaagtgc tgttggtcgc actgctgagc
     1801 tgtactatcc cctttttcgt cgtgctcgca tcctcaagcg ccacggcgga tgcaatatcc
     1861 tctgcccgtt ttgccgctgt ctcagcgcgc gttgctgcag attccgccgt acttttgctc
     1921 tgagctgctg ccgtcgcact accagctgcc tctgtcgcct ttgtggatgc cgtcgtggcg
     1981 ctgcccttcg ctgctgacgc ctgtctggtc gcctcatctt ttgaagcaga cgccgatgat
     2041 gccgatgacg ccgccgaact ggcggacgat gccgctgccg tttttgagga ttctgcacgg
     2101 gtttccgacg ctttcgcgtt cgtttcggat gtcttcgctg cggaagctga cctcgctgct
     2161 gccgtggcct gctcagtggc ttcgccagcc ttcgttgtgg ctgttgaagc ggatgatgcg
     2221 gcgctttctg ccgattttcc ggcggcggtg gcactggctg aggcctgccc ggcacttgtt
     2281 gacgcggcac tggcagacga cgcagccgct gtttttgagc ctgccgcagc tgaggcgctc
     2341 tgtcccgctg ccgtctcaga agacctggcg ttcgtctcgg acgtttttgc cgccttcgcg
     2401 gaatttcctg ccgccgttgc cgaggaagct gcgctactgg cgctcaagga tgcgttcgtt
     2461 tctgatgatt tcgctgcctc ttttgaggcc gccgcatccc gggctgaggt ggcagcttct
     2521 gatgctttcg tgatcgcggt ggatgcagaa gtggcggctg attgttctga cgctgcggca
     2581 ttcgtttctg acgttttcgc cgcaccggca ctggtagctg ccacgctttt tgaggactct
     2641 gcagcggcag cactttttga ggcttcagtg gcctttgttg atgccgttcc tgcgctggaa
     2701 gacgctgact gagccgacga agcggcctgt ccggctgacg tgctggctgc acgtgctgaa
     2761 tctgcagcat cagtcgcatg ggttgccgcc tcacgggcag atgtgccggc atcactggct
     2821 gacttcttcg cggctgccgt gttctgtgcc accacggacg cgttacgcgc tacctcttcc
     2881 accatcagtt caaaacggcg cagtgcctca ggacgggcat catcctccgt catggcaccg
     2941 agaaaatcat tcagcgtacc gggtcgggaa tcttcataca cggtgatggt cccggcatgt
     3001 gacggcggga atccttccac caacagaata acgctgtact gaccgtactc aacgtccatg
     3061 ctgtaacgcc cggcttcatc cggattttct gaggccagcg tgttcaccac caccgtggtg
     3121 ctgttacgtt ttgctttcag ctggattgtg cagttctgta ccggttttcc tgtgccgtct
     3181 ttcagtacac ctgaaatctt tactgccata ttcaccccac aaaaaagccc gcctgaaccg
     3241 gcgggctgtc ataacactgt gttacctggc taatcagaac ttataaccga cacccatgat
     3301 gaaaccgtca gtgcgccagt cgccactgcc ggagccttca taagcaatat caatggccac
     3361 ggattcggtc gggttaaact gcacgccagc tccccacgcc agagacgtgt tgctgtggcg
     3421 accgtcatca cttccggtca gcacgtcgtg cgttttcccc ttgttgtcag ttacgcggag
     3481 ataatccccg gagaaagtcg acacacggct gtaagccaca cccgccatcg catacgcgct
     3541 gaaccattca ttcacgcgca cagacggccc caccatcacg ctgaaccagc ggttacgaac
     3601 ggaatcttca tgccagcggg tatcgctgta acgggtcagc tggcgattct tgtctcctgc
     3661 atagctgaat gacgtcacca gccccagtgt gtccgtaaac tcataacggt atttcacgtt
     3721 aatcccgttc agatcatcgc tgccgggaac gttcgtcgag gcatgaagat accccgcgct
     3781 cagcgtggac tgatgttcag acgcccatgc aggcgcaccg gatacggcca gacaaatggc
     3841 tgcggacaaa atggcggcat aaagtttacg cataattacc tctcgctttt ctgcaataaa
     3901 aaaggcgtca tttctgacgc ccgttatggg ttataaaatt cagctgatac tgatacctgc
     3961 tgtggatttc ttcatcacca caaccagcag atcgctgata ctggttgttg gtgtccagtt
     4021 attcgctcct gatgaagata cggtgaatgt cagtgtcagt gtcccctgtc cggcaggcat
     4081 atctataact gaagaaaata cgccctgagc atccgtcgtg gactgattaa aaatctcctg
     4141 accattgcgg gtcactctta accggcaggt tgaataccag tatgactgtt ggttattact
     4201 gttgaaattc tcatgcttac caccgcggaa taacactggc ggtatcatga cctgccggtc
     4261 aaatttctga tcatcactga ttcttaccgt gatggtgccg ctggcataac tgttcgtgcg
     4321 ggggaaagac ttgctgaccg ttttgacaat atcgccttca atctggttgg ctgacagttt
     4381 ccccttaatc tgacagttct cattaatcgt gacgttgttg agcgtccctg agttcgcatt
     4441 cacactgcca ctgatatccg catttttagc ggtcagcttt ccgtctgatg tcagggaaaa
     4501 taccggagga ctgccaccgc tggtaatggt gggtgccgtc aggcgtttca ggaacacgtc
     4561 gttcatgaat atctggttgc cctgcgccac aaacatcggc gtttcattcc cgtttgccgg
     4621 gtcaataaac gcgatacggt tagcggcaac cagaaactgg ctcagtttgc cttcctccgt
     4681 gtcctccata ctgaggccaa tacccgcgac ataatgtttg ccgtctttgg tctgctcaat
     4741 tttgacagcc cacatggcat tccatttatc gttggcatcc ttccactctt tcgaaaactc
     4801 atccagtctg ctggcgttat cctccgtcag atcgactttt tccagcagct ctttaccgag
     4861 atgggattcg gttatcttgc ctttgaaaaa atccaggtaa ccttccgcat catcgctcgc
     4921 ccgaccgacg gactccacga atgccgattt gccaacggtg ttcacactgc ggatataaaa
     4981 gtaataatca tggcccggtt taatattgat actggcggct atccagtaca gccccgtgcc
     5041 aaggtagcgg gctgtggttt caacctgcct gatatcggta atccgcgttt ccgagaacca
     5101 gaactcaaat tgtaccgtcg gatcataaac cgcaagatgc ggcgtggcgg ttatctgaaa
     5161 atagcccggt gtcagctcaa tccgagacgg cgctgccggt gcggcaatcc ggaacgatac
     5221 cgatgccgga tcgccctgct gtccccacgc atttaccgcc cggacagtca gcctgtagtt
     5281 ccccagcgcc agttgcgtga agcggtatgt ggtttccgtc gtccttgccg tactgaccag
     5341 ccgctcactg ccatcgtccg ctgccacggt caggcgaagc aggaagctca cccccttcac
     5401 caccttcggc gtgtcccagc acgccagcac ctgatattcc ccgctgtctg cggtgacttc
     5461 gacagtcagg tgctgcaccg cgggcggcgt gacgccattc accgtgccgc tcaagtcgcc
     5521 gtcaaagtgc gccccgttat ccacgatggc ttctttttcg ggtacatgct gcacggcggt
     5581 aatagcatac gtgccgtcgt cgttctcacg gatactcacg cagcggaaca ggcgctggcg
     5641 caacgtcggc aacttcagcc cccatacgct gtatccggca acgccgtcag gaacacggct
     5701 cacttttacc ttcacgccgt cggtgacgga ctggacctcc acgctgaccg gattgccact
     5761 tccgtcaacc aggcttatca gcgtggtacc ggaggatggc agcgtgattt cacggtcgag
     5821 cgtcagcgtc cgggtctggc tgttcaccgc cagcacgcgt ccgccgatgc tgatccccgc
     5881 atagtcatca tcgcagattt caataacatc acccggcaca tggcgaagcc cttcggcacc
     5941 cacgctgaaa tccacggtct gcgtctccag cagctccgtt ttaatcagcc acagcccggc
     6001 gcggtgtgcc tgcccccggc tggtacagcc aaaggcatcc atcttcgtga cgttacgacc
     6061 gtaacggaga atggcctgcg tgtcctccac aagctctgtc gccgtctccc agccgttatc
     6121 cgggtcaatc cagttcacct caacggcatt atggcggtcc ttgagggcgc tgaagctgta
     6181 gcggaacggc gcgccatcat ccggcatcac cacattactg cggttatagg tccacacctt
     6241 atccgacggt cggtcctgca cgaacgtcag cgtctgcccg ttccataccg gcatacagcg
     6301 catcgccgag cagaaatcgc tgagaacatc ccacgcctta cgctgtgtgg tcaggtaggc
     6361 attacaggtg atgcgcggct ccgtgccgcc aaagccgtcc ggcactgact ggtcgcagta
     6421 ctggccgatg acatacagcg cccatttatc cacatccgcc gcaccaagac gtttccccat
     6481 gccgtagcgc ggatgggtca gcatatccca cagacaccag gccatgttgt tgctgtatgc
     6541 cggtttaaac gttccgtccc agataccgct gtattgccgc gtctgcgggt tataattcga
     6601 cggcacctgc agaatacgcc cgcgcagatg ataattacgg ctcacctgct ggctgccgaa
     6661 ctgctccgag tccacctgca cgccgaccag tgccgtgttc gggtagcact gtttcacatc
     6721 gataatttcg gtgtatgacg accagagcgt tttgttctgc agctggtctg tggtgctgtc
     6781 cggcgtcatc ctgcgcatcc ggatactgaa cgggcgcggc ggcaggttat ccaccaccac
     6841 cgaggccaga tactgtgaag tggttttacc cttaatggtg atgtcctttt ccgtcaccca
     6901 gccaccgtta cgttgtatct gaaccagcag gcggacttcc gatggattcc tgtccccctt
     6961 tgaggtggtt tccaccagtg cctgcacgcc gaaagtaaaa cgcagacggt caatgtttgc
     7021 cgacgtgatg gtccgggtga tcggcgtgtc atatttcact tctgtaccga gcaccgtctc
     7081 ggagccggag gattcaaatc cctccggcgg tgtctgctcc tgctcaccgg cccggaacac
     7141 caccgtgacg ccggatatat tggtattccc ctcactgttc agcaccggcg tactgttcag
     7201 cagcacgctt tttaatccat ccaccggacc ttcaaccggc ccttcgctga tggcatcgat
     7261 cacactcagc agctgcgtgg acttcaggtt gtccttcgct tcgcgcgggg tatgcccctt
     7321 actgccgcct ttacccattc gtcatgctcc ataaacgata aaaccgcccg gaggcggttt
     7381 cacataaaac attttgcatc agcgaccaat caccacaacc tgaccaccat ccccttcgtc
     7441 tgccgtgctg atctcctgag aaaccacgcg tgaccccacg cgcatttcac cgtacagaac
     7501 cggcagaaca ttgccctggg caaccatgtt atccagtgag gagaaatagg tgttctgttt
     7561 gccgttatcc gttgtctgtg tacggggagt tctggctttc ggtgccagca tctgcgccac
     7621 accaccgagc accatactgg caccgagaga aaacaggatg ccggtcatac ctccggcccc
     7681 aatggctgcc ccccatgctg caagggtggc tccggcggta aagaatgatc cggcaatggc
     7741 ggcagccccc aggacaatct ggaatatgcc acctgacttg gccccggcga ctctgggaac
     7801 aatatgaatc acagcgccat caggcagagt ctcatgtaac tgcgccgtta acccggacgt
     7861 gctgacgtcc cgcccggcaa tccgtacctg ataccagccg tcgctcagtt tctgacgaaa
     7921 caccgggagc tgtgtggcca gtgcgcggat ggcttcagcc cccgttttca cacgaaggtc
     7981 gatgcggcgg ccaaatcgtt gcaaatcccc gtaaaggcag atgcgcgcca tgcccgatga
     8041 cgccagaggg agtgtgtgcg tcgctgccat ttgtcggtat acctctctcg tttgctcagt
     8101 tgttcaggaa tatggtgcag cagctcgcca tcaccacagt aaatggcggc atgattcggc
     8161 accgatgaac caaaacagca cagcagcaca tcgcccggtt gtgctgatga caacggcacc
     8221 tgatacagcc ctgtggcctc cagattatcc agatagagat tctgaccgtg acgccaccag
     8281 tcatcctcgc gatgaaaatc cggcatctca atccccgcca gatgataagc atcccggaac
     8341 agcgtgtaac agtccgtcac cccgtgctca aagcgccgcc cggtgagatg cggcacacag
     8401 cggaacttat gaatcgtccc ccggcagacc agccaccacg gcaaatcact ctgcacctgc
     8461 agccgccggt cggcctcact cagccagggc agaccaccgg ggtggctgtg gaccagcgcc
     8521 acaatctcac cctgcatttc tgcctgcagc cagtcttccg gcgacatacg gaaatacgcc
     8581 tccggctcac cggagatatt cacgcagggg aaatatcttt ccccctccgg cgtgcttacc
     8641 acgaagccgc acgactccgc tggcgcacat cgccgggcgt gcgccagaat cgctgattct
     8701 gtctgtgtca tgggatttac tgcgaaagtt tgttaatgga aaggaagccg ccaaagttgc
     8761 cgacgttatt gcggaactta caaccgctca ggcatttgct gcatttatcc ttcgtgatat
     8821 cggacgtcgg ctggtcatat tcatccgcga ccgccggacc gtgataaccg cactcatcgc
     8881 cgcgataggt ccaggtgcag gtgttggcca gcatgatacg tcccggaaaa acagcgccat
     8941 ccgtttccgt cggcgtggac agtacaaagg aggcactcac cgcgctcagt tcgctgcact
     9001 gctcgatgcg ccagcggctg atcacctcct gctccggatc ggcgtcgctg tttccgttga
     9061 cgaagttcac cgcatccaga aaacgggcgt aaaccttacg ccggaccacc gttccgccga
     9121 ccaaactctg cagatcttcc gccatcccgg tgaccatacc gtacaggtta gaaaccgtca
     9181 gcgtggggcg cgtactggtg cctttgccat tcagttcaag accactcccc tgaatgggat
     9241 atggctgata ctgtcgcccc tgccaggtga ccggctcacc tttttcgttc tgctcattac
     9301 agaaaaaata acgttctcca ccgacctctg tcaggtcgat ttcccatagc accacgctgg
     9361 ccgactgctc cgcacgggtg cattcattca gtgtttcctg ccggatatcc tgcatcagtt
     9421 caccacctgt tcaaactctg cgctgaactc aacacgcagc atactgaccc gcgacgacca
     9481 ttttgcgcag gtcaccttta tctgccgcca ctcataaggc ggcgtccaca gaaaggcttt
     9541 ccagcccccg tgctcttcca gaaacgactc cagtaccgtg gcctcctcac gggggacaga
     9601 aagcgtcacg ctgtacgttt tcaggttggc attcagcccg gcaggcgctc gctgggaata
     9661 gccatcacca aagcgcacct ttcttacaga aggggacgaa gccacatcca taccgggttt
     9721 cactttccag cggaaggttt tcatcgtcca cctccggaga acagaccacc atcgcgcatc
     9781 tgcccggtca caacatccat tgccgcctta cgggctacgt cataaacagc cttcagtgcc
     9841 tgtggcccta tctgaccgtt cgtgccgtcg ttgttaatca ccacatggtt attctgctca
     9901 aacgtcccgg acgcctgcga ccggctgtca gccagactgc ccggtgtacc gacataacca
     9961 ccggtggcat agccgcgcat cagccggtag agattcccca cgccaatccg gctggttgcc
    10021 tccttcgtga agacaaattc accacggtga acaatccccg ctggctcata tttgccgccg
    10081 gttcctgtga atcctccggt tgcaaaatgg aatttcgccg cagcggcctg aatggctgta
    10141 ccgcctgacg cggatgcgcc gccaccaaca gccccgccaa tggcgctgcc gatactcccg
    10201 acaatcccca ccattgcctg cttaagcaga atttctgtca tcatggacag cacggaacgg
    10261 gtgaagctgc gacagttctg ctcactgccg gtcagcatcg ctgcca
//
LOCUS       DAZGSP010000152        10217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZGSP010000152 DAZGSP010000000
VERSION     DAZGSP010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.153"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            29..1711
                     /gene="ydeN"
                     /locus_tag="ACHK74_004643"
     CDS             29..1711
                     /gene="ydeN"
                     /locus_tag="ACHK74_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP0223341.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1763..2920
                     /gene="ydeM"
                     /locus_tag="ACHK74_004644"
     CDS             1763..2920
                     /gene="ydeM"
                     /locus_tag="ACHK74_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP0223342.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKNLFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2886..3008)
                     /locus_tag="ACHK74_004645"
     CDS             complement(2886..3008)
                     /locus_tag="ACHK74_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223343.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKGRVINYSPFVLQHP"
     gene            3211..4896
                     /gene="yddA"
                     /locus_tag="ACHK74_004646"
     CDS             3211..4896
                     /gene="yddA"
                     /locus_tag="ACHK74_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFP0223344.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDIFSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
     gene            4934..7306
                     /gene="yddB"
                     /locus_tag="ACHK74_004647"
     CDS             4934..7306
                     /gene="yddB"
                     /locus_tag="ACHK74_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFP0223345.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7363..10146
                     /gene="pqqL"
                     /locus_tag="ACHK74_004648"
     CDS             7363..10146
                     /gene="pqqL"
                     /locus_tag="ACHK74_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP0223346.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         2998 a         2256 c         2250 g         2713 t
ORIGIN      
        1 attaagtaca ataagaagag gaacatttat gaagtctgca ttaaagaaaa gtgtcgtaag
       61 tacctcgata tctttgatac tggcatctgg tatggctgca tttgctgctc atgcggcaga
      121 tgatgtaaag ctgaaagcaa ccaaaacaaa cgttgctttc tcagacttta cgccgactga
      181 atacagtacc aaaggaaagc caaatattat cgtactgacc atggatgatc ttggttatgg
      241 acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc gtgaagttgt
      301 cgatacctac aaaataggga tagataaagc cattgaagct gcgcaaaaat caacgccgac
      361 gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg cgcacggtgt
      421 ttccggcccc tcccgcgccg caataatgac cggtcgagcg cccgcccgct ttggtgtcta
      481 ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc ctgaattatt
      541 ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa aaatcagtaa
      601 tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca ccacattttc
      661 tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat tccacgctgc
      721 aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg tccccgcaaa
      781 aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc gtgccaaaac
      841 acttgaccag ccatttatgc tttacctggc ttataatgct ccgcacctgc caaatgataa
      901 tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag cagataacta
      961 ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac aactgaagaa
     1021 aaacggacag tatgacaata caattattct ctttacctcc gataatggtg cggttatcga
     1081 tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct atcctggcgg
     1141 tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta attatgacaa
     1201 gctgatttcc gcaatggatt tctacccgac agctcttgat gcagccgata tcagcattcc
     1261 aaaagacctt aagctggatg gcgtttcctt gctgccctgg ttgcaagata agaaacaagg
     1321 cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg acgaggaaaa
     1381 tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg attacccgca
     1441 taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata acgattattc
     1501 gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg atctacagca
     1561 aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag gcgtggtaag
     1621 agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga agtttaacaa
     1681 tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg aattttccgc
     1741 cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt ttcaatgtaa
     1801 tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc atgaaaaatg
     1861 gatggatgac agtactctga aagagttcat caaacaatat atcgcagcgt ctggcaatca
     1921 ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt ttttccgtaa
     1981 agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaatg cattacaaac
     2041 gaatggcatt ttattgaata atgaatggtg tgcttttctc aaagaacatg agtttctggt
     2101 tggtatctcg atcgatggcc cccaggagct acatgaccgt tacagacgca gtaattcagg
     2161 taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat atcaagttga
     2221 gtttaatacg ttaaccgtca ttaataacgt taatgtccat taccctcttg aggtttatca
     2281 ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctcg aaaccgggac
     2341 gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg atttttctgt
     2401 gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg ttaaaaacga
     2461 tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt tggggaatgg
     2521 gcataccagt tgtattttcc aggagtcctg caaggataat ctggttgttg aaagtaatgg
     2581 agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa acattaataa
     2641 atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac ggatttcagc
     2701 gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta agcatcgtat
     2761 tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa accttttttc
     2821 aaccatggta ccttatatga acgccatggt ggagttagct aagaacagag taccgcttta
     2881 tcacattatg gatgttgcaa aacaaatgga gaataattaa taacacgccc ttttagctcc
     2941 attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc tgtcgggtgt
     3001 ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg tcagggcatt
     3061 agcaataccc ttgattatca atagcataac cataactaat gaaaatgaaa tatttcttta
     3121 tatttcatat tgttaagtga tacttaaata aatttcatca gtaaatcatt gccatagata
     3181 atcattggat ggcacaatca attttcgcta atgataacca ttcccattac gttttgtatg
     3241 ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa taataaaact
     3301 tcagtgttgt taatcattat tatcctcgcc atgatccttg gcgtggttaa aattcaggtc
     3361 tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga aaccgataag
     3421 ctttggcagc ttgtcctttg gttccctgcc ctgctgggga tctttgtatt aatatccgta
     3481 aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt aacagattac
     3541 taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta cggcgagcat
     3601 aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact gatcagcaaa
     3661 acgctcagcc tttcatttgg cttcatccag tcacttagca tgctgatcac ctttaccgtt
     3721 attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga atggaatatc
     3781 caggggtata tggtctatac cgttgtgctc attgtgatcg gtggaacttt atttacccat
     3841 aaggttggta agcgtattcg tccgcttaat gtggaaaaac aacgtagcga agcgacattt
     3901 cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag caatgctgag
     3961 tctctacaac gccaggagtt gagcgacaat tttcatacca ttaaagagaa ctggcatcgt
     4021 ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg ctcgcttagc
     4081 gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa tctgggcgga
     4141 ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg gtttatttat
     4201 aaatatgatg aacttgctga actggctgcg gttatcgatc gcttgtatga gttccatcaa
     4261 ctcactgaac agcgccctac gaataagcct aaaaattgcc aacatgcggt acaagtggct
     4321 aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa ctttcatgtt
     4381 tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac cacactgctt
     4441 aaaacattat cccactgctg gccgtggttt aaaggtgata ttttttctcc tgctgacagt
     4501 tggtatgtgt cacaaacacc gttaatcaaa accggcttac tgaaagagat tatttgtaaa
     4561 gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca ggttggtctg
     4621 gggaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct ttccagcggc
     4681 gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg gatatttctt
     4741 gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg tttagtgcgt
     4801 gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt ctggaacctg
     4861 gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc ctgctgtacg
     4921 gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg gcgctgatgt
     4981 ggcgcaggcc gtcgatgaca aaaacatgta catgcatttt tttgaagaga tgacggtcta
     5041 tgctcctgtc cctgtacccg taaacggcaa cacgcattat accagtgaaa gcatcgagcg
     5101 tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg cggtacgcat
     5161 ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga aaatctctat
     5221 tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg caactaataa
     5281 cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg ggatgtcaca
     5341 ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca gttttgtgcc
     5401 ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac gcttcaacgc
     5461 tgatgatagc aaggtgaaat tgggttatcg cactacgcgt tcggactggt taacatcgca
     5521 tatcgatgag aataacaaga gcgcatttaa tcaaggctct tcaggaagta cttattactc
     5581 cccagatttt aaaaagaact tttatacctt gtcgtttaat caggaactcg ctgataactt
     5641 cggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg attatgtttc
     5701 gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata ctgcattgag
     5761 caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa aatataccgg
     5821 ctccagtcgt gattataata ccagcacctt cccgcagtct gatcgcgaaa tgggtaataa
     5881 atcctacggt ctggcatggg atatggatac gcagctcgca tgggccaaac tgcgtaccac
     5941 cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct ggtacaccga
     6001 actttcatgt acatatggtg atattacagg gcgttgcacc cgtggcggat taggacacat
     6061 ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa aattcgccgt
     6121 gggtaatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg atgcgtggac
     6181 tgaacgccat aaccagtctg aatcctatgt gattaatgct gccggaaaga aaactaacca
     6241 taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc tgtatatggc
     6301 ggatcgcatt agctggcgta atgtgtcatt aatgcccggt gtgcgttatg actatgacaa
     6361 ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata tttttgctga
     6421 tcaaacctca atgattacag caggttataa ccgttactat ggcgggaata ttcttgatat
     6481 gggattacgt gatatccgca atagctggac ggaatcggta tcaggcaata aaaccctgac
     6541 gcgttatcag gatttgaaaa cgccttataa cgatgaactg gcaatgggat tgcagcagaa
     6601 aatcggtaag aacgttattg cgcgcgcaaa ctatgtttac cgtgaagcgc atgatcaaat
     6661 cagcaaaagc agtcgtaccg acagcgcgac taaaaccacc attactgaat ataacaacga
     6721 cggcaaaaca aaaacgcatt cgttcaacct cagttttgaa ctggccgaac ccctgcatat
     6781 cagccaggta gatattaacc cgcaaattgt ctttagctat atcaagagca agggcaactt
     6841 gtcgttaaac aatggttatg aggagagcaa taccggtgat aaccaggtgg tttataacgg
     6901 taatctggtt tcttacgata gcgttccagt ggcagatttt aataacccat taaagatctc
     6961 cttaaacatg gatttcacgc atcaaccgag cgggttagtg tgggcgaata cgctggcctg
     7021 gcaagaagcg cgtaaagctc gcattatcct tggtaagaca aatgcgcaat acatcagcga
     7081 atattcagat tacaagcagt atgttgacga aaaactggat agcagcctga cctgggacac
     7141 ccgcttgtcc tggacgccac aatttctgaa acaacaaaac ctgacgatca gtgccgatat
     7201 tctcaatgta ctggatagca aaaccgctgt tgatacaacg aataccggtg tggcgaccta
     7261 cgccagtggc cgtactttct ggcttgatgt cagcatgaaa ttttaatagt tcggatttta
     7321 cttaaccggg taaattcgcc cggttttcgc atggagataa ttatgagaaa cctctgtttc
     7381 ttactgacgt tagtggcaac tctgttgctc cccgggcggc tgattgccgc cgccttaccg
     7441 caggatgaaa agttaattac cgggcaactg gacaatggct tgcgatatat gatttatccg
     7501 catgctcaac caaaggatca ggtaaattta tggctgcaaa ttcataccgg ttcattgcag
     7561 gaagaagaca atgagcgcgg cgtggctcat tttgtagaac atatgatgtt taacggcaca
     7621 aaaacatggc cgggtaataa agtcatcgaa acatttgagt caatgggcct gcgttttggt
     7681 cgcgatgtta atgcctatac cagctatgac gaaacggtgt atcaggtgag tttgccgact
     7741 acgcagaaac aaaatctgca acaagtgatg gcaatcttca gtgaatggag taatgccgca
     7801 acctttgaaa aactcgaagt agacgctgaa cgtggcgtaa ttactgagga atggcgtgcc
     7861 catcaggatg cgaaatggcg cacctctcag gcgcgccgcc ctttcctgct ggctaatacc
     7921 cgtaatttag accgtgaacc tatcggcctg atggatactg tcgccacggt cacaccggca
     7981 caattgcgcc aattttatca acgctggtat caaccaaata atatgacctt tatcgtggtc
     8041 ggcgatatcg acagtaaaga agcgctggcg ctgataaagg ataatttaag taagcttccg
     8101 gctaacaaag cagctgaaaa tcgcgtctgg ccgacaaaag ccgaaaacca cctgcgcttt
     8161 aatatcatta atgataaaga aaaccgggtg aacggcatcg cactctatta tcgcctgcca
     8221 atggtacaag tgaacgatga gcaaagcttt atcgaacaag ctgaatggag catgttagtt
     8281 cagctgttca atcaacgtct gcaggaacgc atacagtcgg gcgagttgaa gactatttct
     8341 ggcggcactg cgcgcagcgt taaaattgca cccgattatc agtcgctgtt tttccgtgta
     8401 aatgcacgag acgataatat gcaggatgct gcgaatgcat taatggcaga gttggcaacc
     8461 attgatcagc atggtttttc tgctgaagaa ctcgatgatg tcaaatctac ccgcctcacc
     8521 tggctgaaaa atgcggttga tcagcaagct gaacgtgatt tacgtatgct gaccagtcgc
     8581 ctggcatcca gctcattaaa taatacgccg ttcttgtcgc cggaagagac atatcaactt
     8641 tcgaaacgtc tgtggcagca aattaccgtg caaagtctgg cggaaaaatg gcagcagtta
     8701 agaaagaacc aggacgcatt ttgggagcaa atggtaaaca atgagcttgc cgccaaaaaa
     8761 gcattgtctc ctgcggctat cctggcgctg gaaaaagagt acgccaacaa aaagctggcg
     8821 gcttacatct tcccaggcag aaatttatcg ttaacagtag acgctgaccc acaggcggaa
     8881 attagcagca aagaaacgct ggctgagaat ctgacatcat taacactttc caatggtgcc
     8941 agggttattc tggcaaaatc cgcgggtgaa gagcaaaagc tacaaattac tgccgtatcg
     9001 aataaaggcg atttaagttt ccctgcgcag caaaaatcac ttatcgcgct ggcaaataaa
     9061 gcagtaagcg gaagcggcgt tggtgagctc tcctcttcca gcctgaaacg ctggagtgcg
     9121 gaaaattcgg taaccatgag cagtaaagtc agtggcatga atacgttgct ctccgttagc
     9181 gcgcggacta ataaccctga acctggtttt cagttgatta accagcgaat cacccacagc
     9241 acgattaacg acaatatttg ggcatcgcta caaaatgctc aaattcaggc gttgaaaacg
     9301 ctcgaccagc gtccagcgga gaaattcgcc cagcagatgt atgagacgcg ctatgctgat
     9361 gaccgcacga aattactgca agaaaatcag attgtacagt ttactgccgc agatgcgctg
     9421 gctgccgatc gccagttgtt ttcatctcca gcggatatca cgtttgtcat tgtcggtaat
     9481 gtctcagaag acaaactcgt ggcgttaatt acgcgttact taggatcaat caaacactct
     9541 gattcgccat tagccgcagg taaaccatta actcgcgcga cggacaacgc atcggttact
     9601 gtaaaagaac aaaatgaacc tgtggcacag gtttcacagt ggaagcgtta tgattcccgg
     9661 acacctgtta atctggcgac gcgtatggcg ctcgatgctt ttaacgtcgc actggcaaaa
     9721 gatctacgtg ttaatattcg tgaacaggca tctggagcat acagcgtttc ttctcgcctc
     9781 tcggttgatc ctcaggccaa agatatcagt catttgctgg cttttacttg tcaaccagaa
     9841 cgacatgatg aactgttaac gttagcgaat gaagtgatgg ttaagcgtct ggctaaaggg
     9901 atcagtgagc aagaactgaa tgaataccag caaaacgttc agcgcagcct cgatatccaa
     9961 cagcgtagcg ttcaacaatt agcgaacact attgtaaata gtcttattca atatgacgat
    10021 cctgcagcat ggactgagca ggagcaattg ttgaaacaaa tgacggtaga gaatgttaac
    10081 actgccgtta aacaatatct ttctcatccg gtaaatactt ataccggagt attattgcca
    10141 aaataataac agccccgtca acacatcgtt ggcggggatt ttagcaatat tcgctatttt
    10201 tatgtaataa ttttata
//
LOCUS       DAZGSP010000153        10086 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZGSP010000153 DAZGSP010000000
VERSION     DAZGSP010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.154"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            370..1347
                     /gene="glaH"
                     /locus_tag="ACHK74_004649"
     CDS             370..1347
                     /gene="glaH"
                     /locus_tag="ACHK74_004649"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFP0223347.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1367..2635
                     /gene="lhgO"
                     /locus_tag="ACHK74_004650"
     CDS             1367..2635
                     /gene="lhgO"
                     /locus_tag="ACHK74_004650"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFP0223348.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAMELREREPNITGLGGIFVPSSGIVSYRNVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2658..4106
                     /gene="gabD"
                     /locus_tag="ACHK74_004651"
     CDS             2658..4106
                     /gene="gabD"
                     /locus_tag="ACHK74_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP0223349.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALSSKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLENGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4120..5400
                     /gene="gabT"
                     /locus_tag="ACHK74_004652"
     CDS             4120..5400
                     /gene="gabT"
                     /locus_tag="ACHK74_004652"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFP0223350.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGNFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5711..7111
                     /gene="gabP"
                     /locus_tag="ACHK74_004653"
     CDS             5711..7111
                     /gene="gabP"
                     /locus_tag="ACHK74_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFP0223351.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPALMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7132..7794
                     /gene="csiR"
                     /locus_tag="ACHK74_004654"
     CDS             7132..7794
                     /gene="csiR"
                     /locus_tag="ACHK74_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFP0223352.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7795..8244)
                     /gene="kbp"
                     /locus_tag="ACHK74_004655"
     CDS             complement(7795..8244)
                     /gene="kbp"
                     /locus_tag="ACHK74_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFP0223353.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8328..8486)
                     /gene="yqaE"
                     /locus_tag="ACHK74_004656"
     CDS             complement(8328..8486)
                     /gene="yqaE"
                     /locus_tag="ACHK74_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFP0223354.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8669..8968
                     /gene="ygaV"
                     /locus_tag="ACHK74_004657"
     CDS             8669..8968
                     /gene="ygaV"
                     /locus_tag="ACHK74_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP0223355.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8978..9502
                     /gene="ygaP"
                     /locus_tag="ACHK74_004658"
     CDS             8978..9502
                     /gene="ygaP"
                     /locus_tag="ACHK74_004658"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFP0223356.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRHEQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPFPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9549..9953)
                     /gene="stpA"
                     /locus_tag="ACHK74_004659"
     CDS             complement(9549..9953)
                     /gene="stpA"
                     /locus_tag="ACHK74_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFP0223357.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2333 a         2583 c         2729 g         2441 t
ORIGIN      
        1 aacaggagaa ccgccgaact atctctattt ataatttaat gttatatctg ccccgataaa
       61 acggggcaga taatatgttt agtttactaa cggtcatttt gcagtgaagc catttactgt
      121 tttttatcga ccagataatc tgttctctaa tgttaactcc ccctaacctg ttgctttagt
      181 tattcatttc ctgtctcact ttgccttaat accctacgtt aaatgttact aatttattgc
      241 ttttgatcac aataagaaaa caatatgtcg cttttgtgcg catttttcag aaatgtagat
      301 atttttagat tatggctacg aaatgagcat cgccatgtca ccctacatct cataagagga
      361 tcgcttctga tgaatgcact gaccgccgta caaaataacg ctgtcgattc aggccaggac
      421 tatagcggat tcactctcat cccgtcggcg caatccccgc gtctgctgga actcaccttc
      481 accgaacaga cgaccaaaca gtttctcgaa caggttgccg aatggcccgt gcaggcgctg
      541 gagtacaaat cgtttctgcg ttttcgggta ggcaaaattc ttgacgatct gtgtgcgaat
      601 cagctgcaac cattgctgtt gaagaccctg ctaaaccgcg ctgaaggtgc gctgttgatc
      661 aatgcggtgg gtatcgatga tgtggcgcag gcagatgaga tggtgaagct ggcaacggcg
      721 gtagcgcatc tgattggtcg ctcaaatttc gatgcgatga gcggtcagta ttacgcgcgt
      781 ttcgtggtga aaaatgtcga taactcagac agctatctgc gtcagccgca ccgcgtgatg
      841 gaactgcaca acgacggcac gtatgtcgaa gagatcaccg attacgtgct gatgatgaaa
      901 atcgatgagc aaaacatgca gggtggaaat tcgttgctgc tgcatctcga tgactgggaa
      961 catctggacc actatttccg ccatccactg gcgcgtcgcc cgatgcgctt tgccgcgccg
     1021 ccgagcaaaa acgtcagcaa agatgttttc catccagtgt tcgacgttga tcaacagggt
     1081 cgcccggtga tgcgctatat cgaccagttc gtccagccaa aagattttga agaaggcgtg
     1141 tggttgagcg agctttccga tgccattgaa accagcaaag gcattctttc tgtacccgtt
     1201 cccgttggca aattcctgtt gataaacaac ctgttctggc tgcacggtcg cgaccgcttt
     1261 actccgcacc cggatctgcg ccgtgaactg atgcgtcagc gtggctattt cgcttacgcc
     1321 actcaccact accagacgca tcagtaagcg caaaggaatt gagcggatgt atgattttgt
     1381 gattattggc ggcggcatca tcggcatgtc gaccgccatg caactgattg atgtttatcc
     1441 ggatgcccgc attgcgttgc tggaaaaaga gtccggcccg gcctgtcacc agacgggcca
     1501 caacagcggc gtgatccatg ccggggttta ttacacgccc ggtagcctga aggcgcagtt
     1561 ttgcctggcg ggaaaccgcg ccaccaaagc cttttgcgat caaaacggca ttcgctatga
     1621 caactgcggc aagatgctgg tcgccacctc tgaactcgaa atggaacgga tgcgcgcgtt
     1681 gtgggaacgc acggcggcaa acggtatcga gcgcgagtgg ttaaacgcca tggaactgcg
     1741 cgagcgcgaa ccgaatatca ccgggcttgg cggcattttt gtgccatcca gcggcattgt
     1801 cagctaccgc aacgtgacgg cggcaatggc aaaaatcttc caggccagag gcggtgagat
     1861 tatctataac gccgaagtca gcggcctcag tgagcataaa aacggcgtgg tgatacgtac
     1921 ccgccagggc ggcgaatatg aagcatcgac gctgattagc tgttccgggc tgatggctga
     1981 ccggctggtg aaaatgctcg gcctcgaacc gggctttatt atctgcccgt tccgcggcga
     2041 gtatttccgc cttgcgccgg agcataacca gattgttaac catctgattt accctattcc
     2101 cgacccggca atgccgtttt tgggcgtgca tctcacccga atgatcgatg gcagcgtgac
     2161 cgtcgggcca aacgcggtgc tggcttttaa acgcgaaggc taccgcaagc gcgacttctc
     2221 gtttagcgac acgctggaaa ttttaggctc gtcggggatt cgccgggtgc tgcaaaacca
     2281 tctacgctca ggactgggcg agatgaaaaa ctcgctgtgc aaaagcggct atctgcggct
     2341 ggtgcaaaag tattgtcccc ggctttcgtt aagcgatctc cagccctggc ccgccggtgt
     2401 gcgggcgcag gcggtatcgc cggatggcaa gttgattgac gattttctgt ttgtcaccac
     2461 cccgcgcacg atccacacct gcaatgcgcc ctccccggca gcgacatcag caattcctat
     2521 tggtgcgcat attgtcagca aggtacaaac gctgttggca agccagagta accccggacg
     2581 cacgctgcga gcggcacgta gtgtggatgc cttacacgcc gcatttaatc aataaccttt
     2641 gaaaacagga cgtaacaatg aaacttaacg acagtaactt attccgccag caggcgttga
     2701 ttaacgggga atggctggac gccaacaatg gtgaagtcat cgacgtcacc aatccggcga
     2761 acggcgacaa gctgggtagc gtgccgaaaa tgggcgctga tgaaacccgc gccgctatcg
     2821 acgccgccaa ccgcgccctg cccgcctggc gtgcgctcac cgccaaagaa cgcgccaaca
     2881 ttctgcgcaa ctggttcaat ttgatgatgg agcatcagga cgatttagcg cgcctgatga
     2941 ccctagaaca gggcaaaccg ctggctgaag cgaaaggcga aattagctac gccgcctcct
     3001 ttattgagtg gtttgctgaa gaaggcaaac gcatttatgg cgacaccatt cccggtcatc
     3061 aggccgataa gcgcctgatt gttatcaagc agccgattgg cgttaccgca gctatcacgc
     3121 cgtggaactt cccggcggcg atgattaccc gcaaagccgg tccggcgctg gcggcaggct
     3181 gcaccatggt gctgaaaccc gccagtcaga cgccgttctc tgcgctggcg ctggcggagc
     3241 tggcgattcg cgcgggcatt ccggctgggg tatttaacgt ggtcaccggt tcggcgggcg
     3301 cagtcggtaa cgaactgacc agcaacccgc tggtgcgcaa actgtcgttt accggttcga
     3361 ccgaaattgg ccgccagtta atggaacagt gcgcaaaaga catcaaaaaa gtgtcgctgg
     3421 agctgggcgg caacgcgccg tttatcgtct ttgacgatgc cgatctcgat aaagccgtgg
     3481 aaggtgcgct gtcctcgaaa ttccgcaacg ccgggcaaac ctgcgtctgc gccaaccgtt
     3541 tatacgtgca ggacggcgtg tatgaccgct ttgccgaaaa attacagcag gcggtgagca
     3601 aactgcacat cggcgacggg ctggagaatg gcgtcaccat cgggccgctg atcgatgaaa
     3661 aagcggtagc aaaagtggaa gagcatattg ccgatgcgct ggagaaaggc gcgcgcgtgg
     3721 tttgcggagg taaagcgcac gaacgcggcg gcaacttctt ccagccaacc attctggtgg
     3781 acgttccggc caacgccaaa gtgtcgaaag aagagacgtt cggccccctc gccccgctgt
     3841 tccgctttaa agatgaagcc gatgtgattg cgcaagccaa tgacaccgag tttggccttg
     3901 ccgcctattt ctacgcccgt gatttaagcc gcgtcttccg cgtgggcgaa gcgctggagt
     3961 acggcatcgt cggcatcaat accggcatta tttccaatga agtggccccg ttcggcggca
     4021 tcaaagcctc gggtctgggt cgtgaaggtt cgaagtatgg catcgaagat tacttagaaa
     4081 tcaaatatat gtgcatcggt ctttaactgg agaacgcgaa tgagcagcaa taaagagtta
     4141 atgcagcgcc gcagtcaggc gattccccgt ggcgttgggc aaattcaccc cattttcgct
     4201 gaccgcgcgg aaaactgccg ggtgtgggac gttgaaggcc gtgagtatct tgatttcgcg
     4261 ggcgggattg cggtgctcaa taccgggcat ctgcatccga aagtggtggc tgcggtggaa
     4321 gcgcagttga aaaaactgtc gcacacctgc ttccaggtgc tggcctacga gccgtatctg
     4381 gagctgtgcg agattatgaa tcagaaggtg ccaggcaatt ttgccaagaa aacgctgctg
     4441 gtcaccacag gttctgaagc ggtggaaaac gcggtgaaaa tcgcccgcgc cgccaccaaa
     4501 cgtagcggca ccatcgcttt tagcggcgcg tatcacgggc gcacgcatta cacgctggcg
     4561 ctgaccggca aggtgaatcc gtactctgcg ggcatggggc tgatgccggg tcatgtttat
     4621 cgcgcgcttt atccttgccc gctgcacggc ataagcgagg atgacgctat cgccagcatc
     4681 caccggatct tcaaaaatga tgccgcgccg gaagatatcg ccgccatcgt gattgagccg
     4741 gttcagggcg aaggcggttt ctacgccgcg tcgccagcct ttatgcagcg tttacgcgct
     4801 ctgtgtgacg agcacgggat catgctgatt gccgatgaag tgcagagcgg cgcggggcgt
     4861 accggcacgc tgtttgcgat ggagcagatg ggcgttgcgc cggatcttac cacctttgcg
     4921 aaatcgatcg cgggcggctt cccgctggcg ggcgtcaccg ggcgcgcgga agtgatggat
     4981 gccgtcgctc caggcggtct gggcggcacc tatgcgggta acccgattgc ctgcgtggct
     5041 gcgctggaag tgttgaaggt gtttgagcag gaaaatctgc tgcaaaaagc caacgatctg
     5101 gggcagaagt tgaaagacgg attgctggcg atagccgaaa aacacccgga gatcggcgac
     5161 gtacgcgggc tgggggcgat gatcgccatt gagctgtttg aagacggcga tcacaacaag
     5221 ccggacgcca aactcaccgc cgagatcgtg gcacgcgccc gcgataaagg cctgattctt
     5281 ctctcctgcg gcccgtatta caacgtgctg cgcatccttg taccgctcac cattgaagac
     5341 gctcagatcc gtcagggtct ggagatcatc agccagtgtt ttgctgaggc gaagcagtag
     5401 ctatgtccgt ttgtaagagt taaacgttgc taagggaatg ataacgatgc agtaaaaaat
     5461 aataacgatg aggcgatcga caaagttaaa aacgactgcg gactgtcccc tcgcccctct
     5521 ggggagaggg ttagggtgag gggtaatcac gcggctacac cctcaccccg gccctctccc
     5581 taagggagag ggagaaaacc ctcccgcttt cgtttttaag attttgttga ccgtctcaat
     5641 taaacgcgtg acccggcgcg ggaaatgtcg gggcgctctc ccaagtgaca cactttcgag
     5701 aggattcagg atggggcaat catcgcaacc acatgagtta ggcggcgggc tgaagtcacg
     5761 ccacgtcacc atgttgtcta ttgccggtgt tatcggcgca agtctgtttg tcggttccag
     5821 cgtcgccatc gccgaagcgg gcccggcggt attactggcc tatctgttcg ccggattact
     5881 ggtggttatg attatgcgaa tgctggcgga aatggcggtt gccacgcccg ataccggttc
     5941 gttttccacc tatgccgata aagccattgg ccgctgggcg ggttatacca tcggctggct
     6001 gtactggtgg ttttgggtac tggttatccc gctggaagcc aacatcgccg ctatgatcct
     6061 gcactcgtgg gttccaggca ttcccatctg gttattttcc ctcgtcatta ccctcgcctt
     6121 aactggcagt aatttattaa gcgttaaaaa ctacggcgaa tttgagttct ggctggcgct
     6181 gtgcaaagtc atcgctatcc tggcctttat tttccttggt gcagtcgcaa ttagcggttt
     6241 ttacccttat gccgaagtga gcgggatctc aagattgtgg gatagcggcg gctttatgcc
     6301 caacggcttc ggtgcggtat taagcgcgat gttgatcacc atgttctcgt ttatgggcgc
     6361 agaaattgtc accattgccg ccgcggaatc cgacacgccg gaaaaacata ttgtccgcgc
     6421 taccaactcg gttatctggc gtatttctat cttctatttg tgctctattt ttgtcgtagt
     6481 ggcgttaata ccgtggaata tgccggggct gaaagccgtt ggttcttatc gctcggttct
     6541 ggaattgctc aatattcccc atgcgaaatt aatcatggac tgcgtgatat tactttccgt
     6601 aaccagctgt ctgaactcgg cgctgtatac cgcgtcaagg atgctctact ccttaagccg
     6661 tcgcggtgat gcgcccgcgc taatgggcaa aatcaaccgc agtaaaaccc cgtatgtggc
     6721 ggtgttactc tccaccggag cggcattttt aacggtggtg gtgaactatt acgcacctgc
     6781 gaaagtgttt aaattcctga tagacagctc cggcgctatc gccctgctgg tttatttagt
     6841 catcgccgtt tcacagttgc ggatgcgcaa aattctgcga gcagaaggaa gcgaaattcg
     6901 cttgcgcatg tggctttatc cctggctcac ctggctggtc atcggcttta ttacctttgt
     6961 gttggtagtg atgctattcc gtccggcgca acagttagaa gtgatctcca ccggcttatt
     7021 agcgataggg attatctgta ccgtgccaat tatggctcgc tggaaaaagc tggtattgtg
     7081 gcaaaaaaca cccgttcata atacgcgctg ataatgatca ggagtcacac catgaccatt
     7141 acgtctctgg atggctatcg ctggctgaag aacgatatta ttcgcggtaa ttttcaaccg
     7201 gatgaaaaat tacggatgag tttgctgaca tcgcgttatg cacttggcgt tgggccgttg
     7261 cgggaagcac tttctcaact ggtggcggaa cggctggtca cggtggtgaa tcaaaaaggg
     7321 tatcgggtgg cgtctatgtc agagcaggag ctgctcgata ttttcgacgc ccgcgccaat
     7381 atggaagcga tgctggtcag tctggcgatt gcccgcggtg gcgatgaatg ggaggcagac
     7441 gttctcgcaa aagcgcatct gctcagtaag ctggaagcct gtgacgccag cgagaaaatg
     7501 cttgatgagt gggatctgcg tcatcaggcg tttcatacag caattgtggc gggctgtggt
     7561 tctcactatt tgctgcaaat gcgtgaacgg ttgtttgatc tggcggcgcg ctatcgattt
     7621 atctggctgc ggcgaacggt gctttcggta gaaatgctgg aggataaaca cgatcagcac
     7681 cagaccctga ctgcggcggt actggcgcga gataccgcgc gcgccagtga gttaatgcgc
     7741 cagcatttac tgacgccaat tcccattatc cagcaggcga tggctggcaa ttaactactc
     7801 ttccggaata cgcaacactt gccccggata aattttatcc gggcttttta gcatcggttt
     7861 attcgcttcg aagattttat tgtacagatt agcgttaccg tagacctgtt tggaaatggc
     7921 actcagagtg tcgccagact taacggtata aaactggctg gcagtggctg gtgtcgccgt
     7981 tttcacctga tcatcgacac tggcaatacc ggaaatattc ccaaccgcaa caaggatttt
     8041 ctccttcgcc tcctgactca ggccgtcacc agtgaccgtc gctttgccgt cggcaatttg
     8101 aatgttcact ttatcggcat caggtatacc ggttttgctc agatgctcct gcaccttctt
     8161 cgcctgatcg tctttatcgt gctgacctgt aaccgcgtcc cagagttttt ctccggcatc
     8221 tttcacaaaa ttgaacagac ccatagctac ctcattcgtt aacggaaaca ctcagaaagt
     8281 gtagcagagg aacgcggcgc gttaacgccg cggggatgag cacaggttta atcgcgggtt
     8341 tgcacccaga acgcgtgaat caaaccgggg atatacccaa gcagtgtcaa cagaatatta
     8401 ataatgaacg cccaaccgaa ccctttaccg agcagcacgc cgagcggcgg cagaatgatg
     8461 gtgatgacga ttctccagaa acccatatgt actccctata agaaaattac tcattgttta
     8521 aaaagagatt ttatctctta agcgtagtaa ttttagcgga gcctgccagt ttcagcttac
     8581 gcttttactc tcatttacgc tgctgaggct ggtatatgtt gcatttttag tttagagttt
     8641 acttaattta gaaaatactt aaataaatat gactgaactc gcgcaattac aggccagtgc
     8701 cgaacaggca gcggccttat tgaaagcaat gagccaccct aaacgattgc tgattctgtg
     8761 catgcttagc ggttcccccg gcaccagcgc gggagagctg acgcgcatta ccggactgag
     8821 tgcctctgcg acatcacagc atctcgctcg tatgcgggac gaagggctta tcgacagcca
     8881 acgggatgcc caacgcattc tatattccat taaaaatgag gcggtaaatg ccattatcgc
     8941 caccctgaaa aatgtctatt gtccgtaagg agtcactatg gctttgacaa ccatttcgcc
     9001 gcatgatgca caagaattaa tcgcacgcgg cgcaaagtta atcgatattc gtgatgctga
     9061 tgaatatctt cgtgaacata ttcctgaagc agatctggct ccattatccg tgctggaaca
     9121 gtcaggtctt ccggctaaat tacgtcacga gcaaattata ttccactgcc aggcaggtaa
     9181 acgcaccagt aataatgccg ataaattagc agcgattgcc gcccccgcag aaatattttt
     9241 actcgaagat gggattgatg gctggaaaag agctggattg ccagtagcgg taaataaatc
     9301 tcaacccttt ccgttaatgc gccaggtgca gatcgctgcg ggtggtttaa tattaatcgg
     9361 cgttgtactg ggttataccg taaatagcgg tttcttctta ttaagtggct ttgttggtgc
     9421 cgggttactg tttgcaggaa tcagcggttt ttgtggaatg gcaaggttgt tagataagat
     9481 gccgtggaac caacgagctt gagaagcgac gccggacgcg ccctagcagc gacatccggc
     9541 ctcagtaatt agatcaggaa atcgtcgaga gatttacctt ctgccagcgc ctgagcaatt
     9601 ggcttcggtg tacggccctg accggtccag gttttagttt caccgttaac atcggtgaat
     9661 ttatatttcg ccggacgcgg ctggcgtttt ttaccagcgc gtggtgcagc agcagagcta
     9721 ttacctaata actcttccgg gttaattccg tcagccttca tcagctccag ccaggtgcta
     9781 attttttcct gacgctcagc cagttcacgc tgctgctgtt cttcttcttc acgtctttct
     9841 ttagtgacaa ccctgaattt ttcgagcatt tcttcaagaa cgtcaatgga gaattcgcga
     9901 gccatcgcac ggagagtgcg aatgttattt aaactttgta acattacgga cataataaag
     9961 aaaacctttt aacgccaaaa caaaaaagta tttcgtcgcg tagtgtaagc gatcacttat
    10021 tatatttcca caaaatttaa tctatttaca gtcctgcgcg agattatttc agctgttatt
    10081 ttctga
//
LOCUS       DAZGSP010000154         9751 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZGSP010000154 DAZGSP010000000
VERSION     DAZGSP010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.155"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..180
                     /locus_tag="ACHK74_004660"
     CDS             <1..180
                     /locus_tag="ACHK74_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223358.1"
                     /translation="LLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAGTPIE
                     AGPFDTVVLFKINYN"
     gene            332..1378
                     /gene="abrB"
                     /locus_tag="ACHK74_004661"
     CDS             332..1378
                     /gene="abrB"
                     /locus_tag="ACHK74_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP0223359.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRFPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(1375..2166)
                     /gene="nei"
                     /locus_tag="ACHK74_004662"
     CDS             complement(1375..2166)
                     /gene="nei"
                     /locus_tag="ACHK74_004662"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFP0223360.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(2202..2936)
                     /gene="pxpA"
                     /locus_tag="ACHK74_004663"
     CDS             complement(2202..2936)
                     /gene="pxpA"
                     /locus_tag="ACHK74_004663"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFP0223361.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(2926..3858)
                     /gene="pxpC"
                     /locus_tag="ACHK74_004664"
     CDS             complement(2926..3858)
                     /gene="pxpC"
                     /locus_tag="ACHK74_004664"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFP0223362.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(3852..4508)
                     /gene="pxpB"
                     /locus_tag="ACHK74_004665"
     CDS             complement(3852..4508)
                     /gene="pxpB"
                     /locus_tag="ACHK74_004665"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFP0223363.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(4531..5274)
                     /gene="ybgI"
                     /locus_tag="ACHK74_004666"
     CDS             complement(4531..5274)
                     /gene="ybgI"
                     /locus_tag="ACHK74_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFP0223364.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVLRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            5545..7026
                     /gene="dtpD"
                     /locus_tag="ACHK74_004667"
     CDS             5545..7026
                     /gene="dtpD"
                     /locus_tag="ACHK74_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFP0223365.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSSMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(7068..8486)
                     /gene="phrB"
                     /locus_tag="ACHK74_004668"
     CDS             complement(7068..8486)
                     /gene="phrB"
                     /locus_tag="ACHK74_004668"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFP0223366.1"
                     /translation="MITHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(8483..8992)
                     /gene="ybgA"
                     /locus_tag="ACHK74_004669"
     CDS             complement(8483..8992)
                     /gene="ybgA"
                     /locus_tag="ACHK74_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFP0223367.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(9134..9751)
                     /locus_tag="ACHK74_004670"
                     /pseudo
     CDS             complement(9134..9751)
                     /locus_tag="ACHK74_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         2203 a         2694 c         2402 g         2452 t
ORIGIN      
        1 ttgctggatg aaaacgcctc gccttatacc ttcaataaat atgctgaata cgccgatttc
       61 accagcgaca tgttggtcta cgaaaaaacc tatacggctg aactttcgtc cattgcaggt
      121 acccccatcg aggctgggcc cttcgataca gtggtgcttt ttaaaattaa ctataactga
      181 tttgccatga tgccctttgc agccggaaag ggcatctctt tcagaaaatt gctgattttc
      241 cccttttcct tgattgctgt tattttttcc tggcccggct ttctttatgc tgtatcgata
      301 caccaaagag aaaataatga gggagcgaag gatgccagtt ttgcagtggg gaatgttatg
      361 tgtgctatca cttctccttt ctattggttt cctcgcggta catctcccgg cggcgctatt
      421 acttgggccg atgatcgccg ggatcatctt cagtatgcgc ggaattactc tgcaacttcc
      481 ccgctccgct tttctcgccg cgcaggccat tcttggctgc atgattgcgc aaaacctcac
      541 tggttcgatt ctcaccacgc tggccgccaa ctggccgatc gtgcttgcta ttttactggt
      601 gacgctgctt tccagcgcca tcgtgggttg gttattggtg cgctatagct cattgcctgg
      661 aaataccggt gcctggggtt cttctcctgg cggcgcggcg gcaatggtcg ctatggcgca
      721 agattacggc gcagatattc gcctggttgc atttatgcaa tatctgcgag tgctgttcgt
      781 cgcgggggcc gctgttttgg tcacccgcat gatgctgggt gataacgctg aagcggttaa
      841 ccagcaaatc gtctggttcc cgccagtgag cattaatctc ctgcttacga ttttgctcgc
      901 ggtcgttgcc ggtacggcgg gatgcttgct acgttttcct tccggcacga tgctcatccc
      961 gatgctggcg ggcgcagtgc tccagtctgg tcagctcatc accatcgaac tcccggaatg
     1021 gctgcttgcg atggcgtata tggcaattgg ctggcggatt ggtcttggtt tcgataaaca
     1081 aatcttactg cgggcactac gcccgctacc gcaaatcctg ctgtcgattt ttgctctgct
     1141 ggctatttgt gcgggtatgg cgtgggggct gacccggttt atgcacattg attttatgac
     1201 tgcctacctc gccaccagtc ccggcgggct tgataccgtg gcggtcatcg ccgcagggag
     1261 caatgccgat atggcgctta ttatggcgat gcaaaccctg cgcctgttca gtattttgct
     1321 gacggggcca gccattgcgc ggtttatttc aacctatgcg ccgaagcggt cggcctagtg
     1381 ctggcagcct ggacaccagt aaaacgggcg cgaggacaac gtggttttct caatgatgcc
     1441 gccacagcgt tcgcacagtt cgccatcacg atgaaaaacc ttaaagcgaa acagcgcccc
     1501 atgatgctta ttctcatcca cctgcccccg cgtagcgtaa gaaaaccgag gaatatccag
     1561 taacgcgtgt gcgagtgcat ccagttgcgc cgcattgagg tctttcgctt tatgatttcc
     1621 agtcaaccca acctgccaga ggatctccac ccgcaaataa ttgccaagtc ctgccagaaa
     1681 cgcctgatcg agcagtaatc cagcaaactg acggttacga aagcgcggtg acaataaccg
     1741 ttcctttacc acctccggcg tcagattcgg atccagcaca tcgggaccaa cgcgttgtaa
     1801 aaacggatgc gtggtcagtt gttccggcct caacatctca atatccgagg cgctataaag
     1861 cagaatggtt ttgtcagccg tttgcagttt tacccgcaat actcgcgtgg tctgcggctc
     1921 ttcgccggta tcaaccacgc gccagacgcc gtaaagctga ttatggctgt agagcgttaa
     1981 gtcgttggaa aaatgagtta acaacgcctt accacgcgtt tccacatggg taacgtgttg
     2041 accgataagt tgtgattgat aagtttttaa ctgcgggaag gcaaaccaga catcagttag
     2101 tggtttgcct ttgatcgccg cctccaggtt atccgctgca cggcggatct ccgggccttc
     2161 aggcatggtg atatcctttt ttcattcttt attaatcggg gttatgctgc gacaacaatc
     2221 tccttttcgg caaatgtaga gcgtagtcgg cgggcgaacg ccagtgcgtg ctcgccgtcg
     2281 ccatgcaggc agactgtttg cgccgtgacc gttgcccatt cgccggtgat acttttgact
     2341 ctgccgtgtt gcaccatctc tagcgtttgc gccagcgcct gttcttcgtt ttcaatcaac
     2401 gcgcctggct gacttcgcgg caccagcgag ccgtcagcct gataaccgcg atcggcaaac
     2461 acttcctcgc gcgttgtcag gccatattgc ttgcctgcac gaatcagctc gcttcctgcc
     2521 agcccaacga gaaccaatgc tggatcgcaa gcgtataccg ctctggcgat ggcgtctgcc
     2581 agttgtgctt ctttcgccgc ctggttgtac aacatgccgt gcggtttgac atgacgcatt
     2641 acgccgcctt gcgcacgggc aatcgttgcc agcgcgccaa tttgatacag cgtctgggcg
     2701 taaacggttt ctggcggcag ctgcatggcg ctgcgaccaa aattttccct gtcgggaaaa
     2761 ctcgggtgag cgccaatcgc gacaccattt tttattgctt cacgcacgca agcctgcatg
     2821 gtttgggcat cgcctgcatg aaatccacag gcaatattgg cagaggaaac cagcgttaat
     2881 agctctgcgt cgctggcgca gccttcgccc agatcggcgt tcaggtcaat tttcattgtg
     2941 tagccgccac gctaattgtt cgaaataacg ttgttgatct tgccgcgctt tcagtgcctc
     3001 ttccagtgaa cactggacaa aatgaatcgg ctgaccgagc ggaatttgcg ccagatggta
     3061 catatcagcc tcaatgatac aggcaatacg cgggtaacca ccggtggtct gtgcgtcgtt
     3121 catcaacaca ataggctgcc cgttatgcgg cacctgcacc acacccggta acaaaccgtg
     3181 agataacagt tcgcgatcgg tggtgcgttt taaaatttgc ccctgtaagc gatagcccat
     3241 gcggttactt tgcgggctaa gctgccaggg cgaacgccag aatgcatcct gcgaggcgcg
     3301 atcgaactca tgatattccg gccccggcaa ggcgcgaatg cggttgcccc acagcagctg
     3361 tttaacgccc tgcgcttcca taaaatcacg cttcgatttg ccaatcggga gtcggtcacc
     3421 atccttcagt aaacggcctt ccagcccgcc aatccccacg ttgagatcgg tgctgcatga
     3481 ccccattacc ggcggaacat caataccacc cgcgaccgcc agataactgc gcatcccgtg
     3541 ctgcgggcgt ttaagcgtta aacgctggcc tgctttcatc ggcaatcgcc agccggtcca
     3601 gacggcatta tcatccagcc gcgcttcgca accggcccct gtcagagcaa accacccatc
     3661 agtttcgaac tcaaccgtta actgaccgag cgtgatctcc agcgcggggg cattggcgtc
     3721 attacccacc agcaggttag caatgcgcag cgcaggcata tccagtgcgc cgcagtggct
     3781 gatacccgac tggcgaaaac cgtgacgacc gccatcctgc acagtggtat acatgcccgc
     3841 acgaataatc ttcagcatac tccctccttc tgcggtacaa agcgcacgct gtctcccgga
     3901 cgtaataaga tgggttcgtc acgcgccgga tcaaacaggc tgagtgaggt atgaccaatc
     3961 aactgccagc cacccggcgt tgccagcgga taaacaccag tctgcggccc gccgatcccg
     4021 acagaacctg ccggaacgag caagcgcggt tcagcgcgcc gtggcgtgtg tagttgttcc
     4081 ggcaaactcc cgagatacgg gaagcccggt tgaaaaccta aaaaccagac cacgtattcc
     4141 acggaggagt gcaattcaac aacctgtttt tcgctcaacc cgcaatgcgc cgcgaccacc
     4201 gccaaatccg gtccgcctgc accaccgtaa accaccggaa tttcaataaa gcgagactca
     4261 ggttccaggg cctcgctctc ctcccaccag cgttgcaaac gctctatggc atccagcgcc
     4321 agcgactcag gattacgcaa aatcaccgtg atattgttca tgccgggaat ggcttcaacc
     4381 acattcggca tatccaccag acgctgcgcc agtcgccaga tccgtttctg gctagccagc
     4441 gtcaccggcg gttccagttc cagcactacc gccgtttcac ctatcagata acaacgcgct
     4501 cgttgcactt tcgatccctc tgattattcg ttatgcagga ttaggaatat caataaaggt
     4561 cacatcaaga tcggtatttt cattcagcca ctcgctcaat gcgcgaatac caccacgttc
     4621 agtggcatgg tgacctgcag cataaaaatg caatccctgc tcgcgggctg aatgaatggt
     4681 ctgttcagaa acttcgccag taataaaagc atccacgcca aaacgcgcgg cgctatcgat
     4741 aaaactttgc ccgccgcccg tgcaccaggc gacgcgcagg accacctcag gtccggtatc
     4801 gccacaccat aacggcttac gtcccagacg cgcttcaatc caggaggcta gttccagtcc
     4861 cggcacaggc atggtcagct ctccccacgg caccaacggc tcaatttcgc ccatgaccgt
     4921 gatccccagt aacgccgcca gctgtgcgtt attgcccagc tcaggatgtg cgtcaagcgg
     4981 caaatgccag ccatacaggt tgatatcatt cgccagcaac gtttttaaac ggttacgctt
     5041 cattccgcga atgaccggag actctccttt ccagaagtag ccatgatgca caatgaccgc
     5101 atccgcgccc agacgcactg cctcatcgag caaagcctgg ctggcggtga caccggtaac
     5161 aattttttgc accgtctctt tgccttccac ctgcaaacca ttcggcgcgt aatcactaat
     5221 cgccgcgctg ttcagttttt cgttaatcag ttgttccagt tcggtgtttt tcatcttcgc
     5281 gccttccgta ttctgatagc tacaccgtta acatagcgtt acctcgccag ttcgtcgata
     5341 gccaatcttt tgacatatta agaaacaaac ccgacgccaa aagacgcaaa ttgtttatat
     5401 catcacgctt cagctaatgt atatttatgc aatcagacag catattgcat aaataatcag
     5461 atctgtatca aagttttaac tttaaggaaa cacaagcacg ccgcgcaact attacgtgac
     5521 gttgccgtca attgtggaat tattatgaat aaacacgcat cacagccgcg cgctatttac
     5581 tatgtcgttg cgctgcaaat ctgggaatat tttagctttt acggcatgcg tgccctgctg
     5641 attctctatc tcaccaatca actaaaatac aacgatactc acgcctacga gttatttagc
     5701 gcctactgtt cgctggtgta tgtcacgcca atcctcggtg gctttttggc ggataaagtt
     5761 ctcggcaatc gcatggcggt gatgctgggg gcgttattga tggcgatcgg tcatgtggtg
     5821 ttgggtgcca gtgagatcca tccgtcattc ctctatctgt ccctggcgat aatcgtttgt
     5881 ggctatggtc tgtttaaatc caatgtcagt tgcctgctcg gcgagctgta tgagccaacc
     5941 gatccgcgtc gtgatggcgg tttctcgctg atgtatgcag cgggtaacgt ggggtctatt
     6001 atcgcaccta ttgcctgtgg ctacgcccag gaagagtaca gctgggcgat gggctttggc
     6061 ctggcggcgg tgggtatgat cgcgggtctg gtcattttct tatgtggcaa tcgtcatttc
     6121 acacataccc gcggcgttaa taaaaaagta ctgcgtgcga caaactttct cctgcctaac
     6181 tggggatggc tgctggttct gctggtggca acgcctgcgc tgattaccgt attgttctgg
     6241 aaagagtggt cggtatacgc cttaattgtc gcgactatta ttggcctggg tgtactggca
     6301 aaaatttatc gcaaagcaga aaaccagaaa cagcgtaagg agctggggct gattgtgacg
     6361 ctcaccttct tcagtatgtt gttctgggcc ttcgcacaac agggcggcag ctcgattagc
     6421 ctttatatcg accgcttcgt taaccgcgat atgtttggtt ataccgttcc gactgctatg
     6481 ttccagtcga ttaatgcctt cgcagttatg ctgtgcggtg tgttcctggc gtgggtggtt
     6541 aaagaaagcg tcgcaggtaa tcgtaccgtg cgcatctggg gaaaatttgc tcttggtctc
     6601 ggcctgatga gcgccgggtt ctgcattctg accttaagcg cccgctggtc ctcaatgtat
     6661 ggtcactctt ctctgccact gatggtatta ggcctggcgg tgatgggctt tgcggaactg
     6721 tttatcgacc cggttgccat gtcgcaaatt acgcgcattg aaatccccgg tgtgaccggc
     6781 gtattaaccg gcatttatat gctgctttcc ggcgcgattg cgaactatct cgcgggcgtg
     6841 attgccgatc agacatcgca ggcttcgttt gatgcttccg gggcgatcaa ctactccatc
     6901 aatgcatata ttgaagtatt tgatcaaatt acctggggcg cactggcgtg tgtaggactg
     6961 gtactgatga tttggctgta tcaggcgctg aaattcagaa accgcgcgct ggcgctggaa
     7021 tcttaatagg ccagcaccat acctgataaa acgctcgctg cgttttatca ggctcctttc
     7081 ctcgcttctt cgtacgctgc cagcgttcgc agccttgcct ctttgtgatc aactatcggt
     7141 tgcggataat ccagcatcac acctgctttc tgcgcccact tccacggctc atgcaccgct
     7201 ttccctggta catcgcgcag ctccggtaac catcggcgga taaactcgcc ctcacggtca
     7261 aatttctcgc cctgagttat cggattgaaa atacgaaaat acggcgcagc atcagtaccc
     7321 gtagaagccg cccactgcca gccaccgtta ttagctgcca aatcaccatc aatcagctgc
     7381 gacatgaaat atcgctcgcc ttcgcgccag tcgatcaaca aatctttcac cagaaaactg
     7441 gctgtaatca tccgtagccg gttatgcatc cagccagtac tgttaagctg acgcatagcg
     7501 gcatcaacaa tcgggtatcc cgttttgcct ttctgccagg cctgtaaatg tgcgggattg
     7561 ctttgccact gcacacgatc cgtccaggca ataaacggac aatgtttaca caacgagggg
     7621 taatacgtca tcagatgacg gtagaactcg cgccagatca gctcactaag ccagacacta
     7681 ccggccccac cgtccagcgc ctgcggctgt tccgccaaca agcgatgcaa acactggcga
     7741 ggcgataacc cgcccgttgc caggctggcg gacaaacgac tggtgccttc cactgccgga
     7801 aaatctcgtt gttgctcata ttctccggca ccgttctggc aaaactggcg taattgcgca
     7861 atcgccgctt tttcttccac cgggaaatgc gcagtatcga aagactgacg aggataattc
     7921 agcgtaatgg atggcgcggg ctctatcgat ccgctactac gaacttttgg cgcagcgaca
     7981 cactccggca tcccttcccg cagccgtttc agccaggcat tcttaaaagg cgtaaagact
     8041 ttgtacatct cgtgattacc ggtcatcacc gcgccaggcg gcaggatcac gctgtcatca
     8101 aatccttcac acaccacgtt acgcagcgct ctttcaactt ccacatcccg cgcccgctca
     8161 ttcacttcat actgatagtt ataaaacagg tgggtaacgc tgttttccgc gcacacctgt
     8221 ttaactattt cgacactggc gacaaagtca tccacttcac ggaacaataa aggaatacct
     8281 ttttccgcaa gcgctatttg tagcccattc agttgagcat tgataagttc agcctgacgc
     8341 ggcgacatgt tatgcgtcgc ccactggcgt ggtgtagcga tatataacgc cagcacgcgt
     8401 gcagacgaat tgcggcaggc ggcagccagt gcgagattat cgtgcagacg taaatcctgg
     8461 cgaaaccaga ccagatgggt aatcataaaa ctcctgaatg gcgcaagtta atcacacgcg
     8521 gccaaagttc gaaataacgc tgccctgaaa gccaggcgtc aggataaagc gccatatagt
     8581 gtttgatacg catcagcggc gcaagaagtg gttgctcgcc acggcgataa ctgtcgataa
     8641 gcgccgccaa ctgctggcgt tctgtggaat caatatgcgg gcgaaaataa ccctgaacgt
     8701 gcatcaaaac gttggtgtga tcgcgcgggt tggcgggatg agaaagcaaa acaattacgc
     8761 gttggtagta ctggttgtaa aaatcatcca gattttgcca ctggtgaata tcagccacga
     8821 acgggccaag tttgcggtac tccggctgag aatgcgccag aaagacgagt ttatagcggc
     8881 tgtgataatc gagaagcgcc ccgcgcgtta agccatgttc tttcagtcga tgtaactcat
     8941 gaagggcgaa gatacggatt agggtgctgt catcaaatcg ttgctgattc attactgctc
     9001 ctgaacggtt tacaggagta agtgtagacg ttgagaaatg atatttgtga agttaatcct
     9061 gttgtagcag gacaaattta cgaaagcctg ctccccgcaa ggactgacgt caggtagttt
     9121 ctaaccatag ctgcccttta tattcaaacg tgaaatcaat aagctcatca gggacatcgc
     9181 aaacaatatg aagacgggtt tcttctctgc cgtgactctt ttcactaatt gcgtaactgt
     9241 catgcttggg attatttaat tctttcagcg gaaatttttc ctcgaaggct ttatttagcc
     9301 gcccctggtt tccttttaca gcgaataaat aatcacctcc ctgtttttgt atcttctctg
     9361 caatatcttt ctggcaaccc atcgcatcgg ttgtgatgat ttttccttta atatccagca
     9421 tgttaagaag ttcagggata gctgtgatct cattggattt cttatccgtc ttgatctgcc
     9481 cgatgaccag actgtgcatt gttgagaacg cactaatgac atgaatcgct cccctgcggc
     9541 gactcttgtc ataagagtgc cggagcgttt ttccatcaat tgcaatgacg tctttatcat
     9601 ttgatgaatg gcaatcacgc atccagttaa taaagcactc gtgaaatttt gcaggactga
     9661 tacaggatac aactctggca atagtatcgt gaacaggaat accattttca aaatcaccat
     9721 attgcttcaa gaaatcgaga tgtgtttccc c
//
LOCUS       DAZGSP010000155         9468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZGSP010000155 DAZGSP010000000
VERSION     DAZGSP010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.156"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            405..686
                     /gene="drpB"
                     /locus_tag="ACHK74_004671"
     CDS             405..686
                     /gene="drpB"
                     /locus_tag="ACHK74_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFP0223368.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
     gene            726..1421
                     /gene="yedJ"
                     /locus_tag="ACHK74_004672"
     CDS             726..1421
                     /gene="yedJ"
                     /locus_tag="ACHK74_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFP0223369.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTVRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            1488..2906
                     /gene="dcm"
                     /locus_tag="ACHK74_004673"
     CDS             1488..2906
                     /gene="dcm"
                     /locus_tag="ACHK74_004673"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFP0223370.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2887..3357
                     /gene="vsr"
                     /locus_tag="ACHK74_004674"
     CDS             2887..3357
                     /gene="vsr"
                     /locus_tag="ACHK74_004674"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFP0223371.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3346..4266)
                     /gene="yedA"
                     /locus_tag="ACHK74_004675"
     CDS             complement(3346..4266)
                     /gene="yedA"
                     /locus_tag="ACHK74_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFP0223372.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAAVIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4439..5356
                     /gene="yedI"
                     /locus_tag="ACHK74_004676"
     CDS             4439..5356
                     /gene="yedI"
                     /locus_tag="ACHK74_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFP0223373.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            5435..5617
                     /gene="yodC"
                     /locus_tag="ACHK74_004677"
     CDS             5435..5617
                     /gene="yodC"
                     /locus_tag="ACHK74_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFP0223374.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            5788..7482
                     /gene="dgcQ"
                     /locus_tag="ACHK74_004678"
     CDS             5788..7482
                     /gene="dgcQ"
                     /locus_tag="ACHK74_004678"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFP0223375.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWKIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(7479..8294)
                     /gene="yedP"
                     /locus_tag="ACHK74_004679"
     CDS             complement(7479..8294)
                     /gene="yedP"
                     /locus_tag="ACHK74_004679"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFP0223376.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIVENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(8592..8819)
                     /gene="yodD"
                     /locus_tag="ACHK74_004680"
     CDS             complement(8592..8819)
                     /gene="yodD"
                     /locus_tag="ACHK74_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFP0223377.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            8982..9170
                     /gene="dsrB"
                     /locus_tag="ACHK74_004681"
     CDS             8982..9170
                     /gene="dsrB"
                     /locus_tag="ACHK74_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFP0223378.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(9214..>9468)
                     /gene="rcsA"
                     /locus_tag="ACHK74_004682"
     CDS             complement(9214..>9468)
                     /gene="rcsA"
                     /locus_tag="ACHK74_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFP0223379.1"
                     /translation="ETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISDQMNIKAK
                     TVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
BASE COUNT         2348 a         2284 c         2540 g         2296 t
ORIGIN      
        1 tagggggtat caatgattgt tgcgccaaac ggctggtgtg atcaggcgca cattaatgaa
       61 ggcataaaaa aaagccggca tcatgccggc ccaaaaaata taaacaagac gataaaaggc
      121 taataacgga ggcatcatga cacagaaaaa taaaaaggta taattgtatt cagtgctaat
      181 atattgatta aatgaattaa taaagccgat ctcaaagata aataaaatcg ctcaaaacga
      241 tttttcctgt attcactgcc gttgcgcaaa aattatctat ttgttcaaaa aatgagtgag
      301 tcttgactgg ctcatccaat gtggaaaaat gtgactttta tcacataata gtactaagtc
      361 tgaattttcc gggttatctc aaaatggaat acggttcgac aaagatggaa gagagactct
      421 ctcgcagccc tggcggcaaa ctggctttgt gggctttcta tacatggtgt ggctatttcg
      481 tctgggcaat ggcgcgttac atctgggtga tgagccggat ccccgatgcc cccgtgtcgg
      541 gatttgaaag cgatttaggc tctaccgcgg gaaagtggtt aggggcactg gtcggatttt
      601 tatttatggc tctggtcggg gcattgttag gcagcattgc ctggtacacc cgacctcgtc
      661 ccgcacgtag cagacgctat gaataaaaac tgctaaggtg gcgactcttt ggcaacggag
      721 cgcgtatgga cttacaacac tggcaggcac agttcgaaaa ctggttgaaa aaccatcatc
      781 agcaccagga cgcggcccat gacgtgtgtc attttcgccg cgtctgggct acggcacaga
      841 agctggcggc agatgacgat gtcgatatgc tggtgatttt aaccgcctgt tattttcacg
      901 atattgtgag cctggcgaaa aatcatccgc aaaggcagcg ttcttcaatc ctggcggcag
      961 aagagacgcg tcgcctgctc cgtgaagagt tcgtgcaatt tccggcggag aaaattgagg
     1021 ccgtttgtca tgcaattgcc gctcacagtt tcagcgcgca aattgccccc ttaacaacgg
     1081 aagctaaaat cgtccaggat gcggatcggc tggaagccct gggagcgatt ggcctggcgc
     1141 gcgtgtttgc cgtttcaggg gcattgggcg tggcgctgtt tgatggcgaa gatccgttcg
     1201 cacagcatcg cccgcttgat gataaacgct acgctctgga tcatttccag actaagttgc
     1261 tgaagctacc gcaaaccatg caaaccgtaa gaggcaagca gctggcgcag cacaacgcgc
     1321 attttttagt cgagtttatg gcgaagctca gtgccgaact ggcgggggag aatgaaggtg
     1381 tcgatcacaa ggtgatagat gcgttttcac ccgcgggcta agcgcgtggc cctaaatggc
     1441 tgtaattatg ttaacctgtc ggccatctca gatggccggt taaatctatg caggaaaata
     1501 tatcagtaac cgattcatac agcaccggga atgccgcaca ggcaatgctg gagaaactgc
     1561 tgcaaattta tgatgttaaa acgctggtgg cgcagcttaa tggtgtgggt gagaatcact
     1621 ggagcgcggc aattttaaaa cgtgcgctgg cgaatgactc ggcatggcac cgtttaagtg
     1681 agaaagagtt cgcccatctg caaacgttgt tacccaaacc accggcacat catccgcatt
     1741 atgcgtttcg ctttatcgat ctatttgccg gaattggcgg catccgtcgc ggttttgaat
     1801 cgattggcgg acagtgcgtg tttaccagcg aatggaacaa acatgcggta cgcacttata
     1861 aagccaatca ttattgcgac ccggcgacgc atcattttaa tgaagatatc cgcgacatca
     1921 ccctcagcca taaagaaggc gtgagtgatg aggcggcggc ggaacatatt cgtcaacaca
     1981 ttcctgaaca cgatgtttta ctggccggtt tcccttgtca gccattttcg ctggctggcg
     2041 tatcgaaaaa gaactcgctc gggcgggcgc acggttttgc ctgcgatacc cagggcacgc
     2101 tgttttttga tgtggtgcgc attatcgacg cgcgtcgtcc ggcgatgttt gtgctcgaaa
     2161 acgtcaaaaa cctgaaaagt cacgaccagg gtaaaacgtt ccgcatcatc atgcagacgc
     2221 tggacgaact gggctatgac gtggctgatg cagaagataa cgggccggac gatccgaaaa
     2281 tcatcgatgg taaacatttt ctgccgcagc accgtgaacg catcgtgctg gtgggttttc
     2341 gtcgcgatct taatctgaaa gccgatttta ctctgcgtga tatcagcgaa tgtttccctg
     2401 cacagcgagt gacgctggcg cagctgctgg acccgatggt cgaggcgaaa tatatcctga
     2461 cgccggtgct gtggaagtac ctctatcgat atgcgaaaaa acatcaggcg cgcggtaacg
     2521 gcttcggtta tggaatggtt tatccgaaca atccgcaaag cgtcacgcgt acgctgtctg
     2581 cgcgttatta caaagatggc gcggaaattt taatcgatcg tggctgggat atggccacgg
     2641 gtgagaaaga ctttgacgat ccgctgaatc agcaacatcg tccacgtcgg ttaacgcctc
     2701 gggaatgcgc gcgcttaatg ggttttgaag cgccgggaga agcgaaattc cgcattccgg
     2761 tttcggacac tcaggcctat cgccagttcg gtaactcggt ggtcgtgccg gtctttgccg
     2821 cggtggcaaa actgcttgag ccaaaaatca aacaggcggt ggcgttgcgt cagcaagagg
     2881 cacaacatgg ccgacgttca cgataaggcc actcgcagca aaaatatgcg cgcgattgcc
     2941 acgcgtgata cggcgataga gaagcgcctc gccagtctgt taaccgggca gggcctggcg
     3001 tttcgcgttc aggacgccag tctgcccgga cgcccggatt ttgtcgttga tgaatatcgc
     3061 tgcgtgatat ttacccacgg ctgcttctgg catcatcatc actgctatct gtttaaagtg
     3121 cctgcgactc gaaccgagtt ctggctggag aagataggta aaaatgttga acgcgatcgc
     3181 cgcgatatca gtcgtttgca ggaactcggc tggcgcgtac tgattgtctg ggagtgtgca
     3241 ttacgtgggc gcgagaagct gacggatgaa gcgcttaccg agcgtctgga agagtggatc
     3301 tgcggcgaag gtgccagcgc gcagatcgac acgcagggga ttcatttact cgcttgatgc
     3361 gtcctgaata actggcgcaa ctacgggttt tgccgggaag aggtattttc ccaacgtgac
     3421 cagtaccacc gcgaagacaa ttacgccgag cgccagccat tcaatcttcg acagcgtttc
     3481 tccacccagt cccgtaccca gcaagaccgc gaccaccggg ttaacgtagg cgtagctggt
     3541 ggcgagagcc ggactgacgt tgcggattaa atacatataa gcgttaatgg cgataatcga
     3601 accaaacagc gccagatagc caaccgcaag gaagcctgaa agggaaggga gcgccgtcag
     3661 tttttcaccc gcaatcatcg acgcgatcat taacaccacg cctgccgcca gcatctcaat
     3721 cgcacccgcc atcatcccta caggtaaggt aatgcgcgag ccataaaccg agccaaacgc
     3781 ccagctaatc gagccgatta aaatcagaat cgcgccccac ggattgccgc ttaaatttcc
     3841 accgctattg agcatgatga ttccggcaag cccaatggcg atacccaccc attccagttt
     3901 gcgcgtttta atgccaaaca ggcggctgaa gcacagggta aagaggggca cggttgcaac
     3961 cacaacggcg gcgatgccgg aaggaacatt ttgatgctcg gcaaccgtca ccatgccatt
     4021 accgacagcc agcaataaca ggccaatcac cgcggcattg agcagcggac gtagcggggg
     4081 gagtttgtgt ccgcgcagta gcaaaaatgc cagcaataaa ataccggctg ccaggaatcg
     4141 aacgcccgcc atcattaacg gaggccagct ttccacgcca atccgaatga caaaataggt
     4201 tgagccccaa atgatataca acgcaaacag cgcgccaaaa agcggtaaca actggcggaa
     4261 acgcataatc cctcacggtg gaaataaaaa ggtggttcat agtaaacgtg aaaatcattc
     4321 tgctggcgag agatataatt gcacttgatt gttaaaaaaa tgttgacctg tgaagcaagt
     4381 atcagagctg ccgtttttgc ttcatactta cacccttcaa caataaaaat gagagggaat
     4441 gcttttggcc gggagtagtt tactgacgtt gctcgatgat atcgccacac tactggacga
     4501 tatctccgtg atgggcaaac tggcggcgaa gaaaaccgcc ggtgtattag gggatgactt
     4561 atcgctcaat gcgcaacaag tttcaggcgt gcgggccaac cgggaacttc ccgtggtctg
     4621 gggcgtggcg aaaggatcgc tgattaataa agtgattctg gtgccgctgg cgctgatcat
     4681 cagtgcgttt atcccgtggg cgattacgcc tctgttgatg attggtggcg cgtttctctg
     4741 ctttgaagga gtagagaaag tgctgcatat gctggaggcg cgtaaacata aagaagatcc
     4801 ggcgcagagc cagcagcgtc tggagaagct ggcggcgcag gatccgctga agtttgaaaa
     4861 ggacaaaata aaaggggcga ttcgtaccga ttttatattg tctgccgaaa tcgtcgccat
     4921 cacgctgggg attgtggccg aagcgccgtt gcttaatcag gtgctggtgc tttcaggcat
     4981 cgcgctggta gtgaccgtgg gcgtctacgg tctggtaggg gttatcgtta agattgatga
     5041 cctggggtat tggctggcgg aaaaatccag cgcgctgatg caggcattag gtaaagggtt
     5101 attgattatc gcgccctggt tgatgaaagc gttatcgatt gtcggcacgc tggcgatgtt
     5161 cctcgtcggc ggcgggattg tggtacatgg tattgcgccg ttgcatcacg ccattgaaca
     5221 tttcgccggg cagcaaagtg cagtggtggc gatgatatta ccgactgttt taaatctgat
     5281 tcttggattt atcatcggcg gcatcgtggt gctgggagtg aaagccgtag cgaaaatacg
     5341 cggtcaggca cattaatact cgcacaggct attatgcaaa attcgccatc ttcaactaag
     5401 gtgaacaagt ttcactcgaa aaaaggaggc gagtatgagc tttatggtta gtgaggaagt
     5461 tacggttaaa gagggcggcc cgcggatgat tgtcaccgga tactccagcg gtatggttga
     5521 gtgtcgttgg tatgacggtt acggggtcaa gcgggaagct tttcatgaaa ccgagcttgt
     5581 tccgggggag gggagtcgtt ctgcggaaga agtttgaaag ggtatcatta acaccaggcc
     5641 ggatcacatc cggcctggtg tgccaatgcc tgcaatgaat gatagtcgcg tcttatcatg
     5701 ccaccattat aaaattatcg ctgttttgcg gtacgctatc gccaggttat gccagaatca
     5761 taaaaaagca ggttgggagt cgtcagggtg cagcacgaga caaaaatgga aaaccagagc
     5821 tggttgaaaa aactcgcacg ccgcctgggg cctggtcatg tcgttaatct ctgctttatc
     5881 gtggtattgc ttttttccac cttgctcacc tggcgtgaag tagtggtgct ggaagatgcc
     5941 tatatctcca gccagcgtaa tcatctggaa aacgtagcca acgcgctcga taagcatttg
     6001 cagtataacg tcgacaaact gatctttttg cgtaatggca tgcgcgaagc tctcgtagcg
     6061 ccactggatt tcacctctct gcgtgatgct gtaaccgagt tcgaacagca tcgcgacgag
     6121 cacgcctgga aaatcgaact caaccgacga cgcaccctgc cagtcaatgg cgtatcggat
     6181 gcattagtca gcgagggaaa tctcctgtct cgcgaaaatg aaagcctcga caatgaaatt
     6241 accgctgcac tggaagttgg ttacttgctg cgactggcgc acaactcctc gtcgatggtt
     6301 gaacaggcga tgtatgtctc gcgtgccgga ttttacgttt cgacgcagcc gaccttgttt
     6361 acgcgcaatg ttccaacgcg ttattacggc tatgtcaccc aaccctggtt tatcggccat
     6421 tcacaacgag aaaatcgtca ccgcgcggta cgctggttca cttcgcaacc ggaacacgcc
     6481 agcaatactg aaccgcaggt taccgtcagt gttccggtag acagtaataa ctactggtat
     6541 ggcgtgctgg ggatgagtat tcccgtacgt actatgcagc aatttttaag aaacgccatc
     6601 gataaaaacc tcgatggtga gtatcagctc tatgacagta agctgagatt tttgacctct
     6661 tccaatcctg accatccaac agggaatatt tttgatcctc gtgaactggc cttgctggcg
     6721 caggcgatgg aacatgacac gcggggcggc attcgtatgg acagtcgcta tgttagctgg
     6781 gaacgtctgg accatttcga cggtgtgctg gtgcgcgtcc atacgctaag cgaaggcgtg
     6841 cgcggcgatt tcggcagtat cagcattgca ttaaccctgc tgtgggcgct ctttaccacc
     6901 atgttactca tctcctggta tgtgattcgc cggatggtta gcaacatgta tgttctgcaa
     6961 agctcgttgc agtggcaggc gtggcacgac accttaaccc gtttatataa tcgtggcgca
     7021 ctgtttgaaa aagcccgtcc gctcgcgaaa ttgtgtcaga cgcaccaaca tcctttttct
     7081 gtcattcagg tcgatcttga ccattttaaa gcgattaatg accgctttgg tcatcaggcg
     7141 ggcgaccgtg ttctttctca tgctgccgga ttaattagca gttccttgcg tgcgcaggac
     7201 gttgccgggc gggtcggtgg tgaggagttt tgtgtgattc tgccaggcgc gagtctgacg
     7261 gaggctgcgg aagtcgcaga acgtattcgc ctgaagttaa atgaaaaaga gatgttgatt
     7321 gctaagagta cgacgatacg catcagtgcc tcgttggggg taagtagcag cgaggaaacc
     7381 ggtgattatg attttgaaca actccagtca ctggccgacc gtcggcttta tctcgctaaa
     7441 caggctgggc gtaatcgggt attcgcgagc gataacgctt aacgggcgga gaaaaaatgg
     7501 tccagccctt cacgccatcc ttccggtcct tcacgctgcg ttcgccagac gcgggccgga
     7561 tcctcatcat gcagatgcac cccttcacgg tttagccctt tcacaatcac cgcgtaatcc
     7621 attacctcca gtaagggcgc atcgtttggc ccatcgccca ggccaagtgt ggttgggcgt
     7681 ttgcctgaca attgttgata ggtcgcgata atccagttgg cagcctgatc ttttccggca
     7741 gaggcatcca ggacgtgcca gaagcgcgca ccttgcataa actgtaagcc cagttcgttc
     7801 agacgagcgg taaattgtgc catacgctcg tcactgtcgc gccagattag cgttaccgac
     7861 gcctcatgaa gctgcgtcag cgccgcctgg ctacggctta atcccgtcca ttcggctatg
     7921 gttgcatcgt cgacatcatc gaaagtcgtg aatttaaaat gttctttctc gcgtagcgta
     7981 tttaaaacct ggctgatttc gccatggcta atacctgaga tgatgcgtgg aaaaccgtct
     8041 atatcctgcc attgttcagc aagctggatc actgcgccat tttcgacaat cagtggtaac
     8101 ccttgtagcc ccaacgtttt ttgcaagtac agcatttccg ctgaagtttt actgctacag
     8161 agaatgacgg gaacattcgc ttcgcgtaaa cggctgagcc agggggcagc cggttgccag
     8221 tcataactat gactgtccag cagggtgcca tcaagatcgc taaaaaccag tagtggttgt
     8281 tgaattgaaa acatggtcgc gcagttctcc ccttaccagg atgattctca aaacagcaca
     8341 aataatcaaa gatgattttt tcgggagcga tcccgttgac gccagagcca ccaaggggtg
     8401 acgcacgtca catttctatt cataagtagc gttaatcatt catatcgcga atattttctt
     8461 gtcagcgaaa aaaattgcgg ataaggtgat gaacacatca gatttcctgg tgtaacgaat
     8521 tttttaagtg cttcttgctt aagcaagttt catcccgacc ccctcagggt cgggattttt
     8581 ttattgtgca ttcaacgatt cacttcagag acgctgaagt catgaatatc cagttcgaag
     8641 gcatccgcca attcacgcca tgtatgatat tcacgtccat cgacgctaac gtgttcagaa
     8701 tcgttctggc tgcgatgaac ttcgctttcg ctaatttcat tgatcgccgc cagcagggca
     8761 tcgacatcga cgctgacctc acgttttgcg gtatcgctgt actcttttgc ggttttcatc
     8821 atctttcctc gcaaccgtta ctctgttgtt gccgccagtc gttaagtccg cctggcatct
     8881 cttaagtata gaccgtcgag aaaatcagca ctcgcgcgcg cctggcgctg ccagaaaaac
     8941 gaaattgttc tacactggca caaagccaca ggaggaaaac gatgaaggtg aatgatcggg
     9001 taacagtcaa aacggatggc ggtccgcgtc gtcctggcgt ggtactggca gttgaggagt
     9061 ttagtgaagg cacaatgtac ctggtttcgc tggaagacta cccgctcggc atctggttct
     9121 ttaatgaagc agggcatcag gacggtatct ttgtggagaa agcagagtaa tcttattcag
     9181 cctgactggt gggaaaccac cagtcagaat gtgttagcgc atgttgacaa aaataccatt
     9241 agtcacatta tccgtcagtc ggacgacatg gtagataacc tgtttattat gcgttttgat
     9301 cttacgttta atattacctt tatgcgatga aacggtcttg gctttgatat tcatttggtc
     9361 agagatttga atcgttccct gacctgccat ccacattcgc aacatactcg attcggttcg
     9421 gctcaatgat aacgtcggca tatttaaaaa agaggttatc gttgtctc
//
LOCUS       DAZGSP010000156         9170 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZGSP010000156 DAZGSP010000000
VERSION     DAZGSP010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.157"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACHK74_004683"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACHK74_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFP0223380.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTGRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="ACHK74_004684"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="ACHK74_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFP0223381.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHK74_004685"
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHK74_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFP0223382.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLCTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5797..6906)
                     /gene="mdtE"
                     /locus_tag="ACHK74_004686"
     CDS             complement(5797..6906)
                     /gene="mdtE"
                     /locus_tag="ACHK74_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFP0223383.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7014..7292
                     /locus_tag="ACHK74_004687"
     CDS             7014..7292
                     /locus_tag="ACHK74_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223384.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="ACHK74_004688"
     CDS             complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="ACHK74_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFP0223385.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8619..>9170)
                     /gene="hdeD"
                     /locus_tag="ACHK74_004689"
     CDS             complement(8619..>9170)
                     /gene="hdeD"
                     /locus_tag="ACHK74_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFP0223386.1"
                     /translation="TILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFVSGDILST
                     VVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPELGIFAIAA
                     FIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVSVTLVSTL
                     VGIELIFSAASLFSFASLFVKQQ"
BASE COUNT         2467 a         2120 c         2119 g         2464 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgga aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact ggccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgcagtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagttttttc gctaagaaac
     1501 agtctggcaa catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagca ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacacag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgcg ccgctcataa aggccatcgg cataaacact gcggaaagaa caacagcaat
     4441 accgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggtggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 tagccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgct ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcagaatatt actgctcgac ttatcaacgc taatcccctg
     5401 ctgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtct ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atacattctg
     6061 gcggctacct tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg
     6121 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga
     6181 gaatttcagc gtgccggtct ggctgtagcg tttaccattt tccagattga gctgtactgg
     6241 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgtaagaa
     6301 atcctgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
     6361 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
     6421 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
     6481 cgctttggcg acggtgacat tggcttctgc ttcattcaac tgggtgcgcg cggtgtcgta
     6541 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
     6601 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
     6661 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
     6721 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
     6781 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac
     6841 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
     6901 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
     6961 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
     7021 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     7081 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     7141 gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat aaagtcgttt
     7201 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     7261 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     7321 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     7381 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     7441 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     7501 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     7561 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     7621 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     7681 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     7741 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     7801 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     7861 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     7921 tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     7981 acgcactcca tatattgatc ctaagtaaga atcctggttg ttatcaattt gtaaatatca
     8041 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     8101 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     8161 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     8221 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     8281 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     8341 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     8401 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     8461 aaaaaaatta gatgaaattc agtaggttga aatgatcact agcaggtaat tatttcaatg
     8521 atagtgcgca atttatctac aacactgcgt agcggagaga gtattaatcg gatcatagtc
     8581 acatcaagtg actatgatcc gggtgacaac cgggataatt attgctgctt aacgaacaaa
     8641 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     8701 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     8761 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     8821 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     8881 atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     8941 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     9001 agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     9061 acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc aataaactgg
     9121 attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt
//
LOCUS       DAZGSP010000157         9099 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZGSP010000157 DAZGSP010000000
VERSION     DAZGSP010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.158"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1316
                     /locus_tag="ACHK74_004690"
     CDS             <1..1316
                     /locus_tag="ACHK74_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFP0223387.1"
                     /translation="AQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALF
                     FLNAVIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKS
                     NDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNL
                     SSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIAL
                     LCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVH
                     DVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIV
                     DIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPE
                     QMTISIGTFFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            1497..2987
                     /gene="dgcJ"
                     /locus_tag="ACHK74_004691"
     CDS             1497..2987
                     /gene="dgcJ"
                     /locus_tag="ACHK74_004691"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFP0223388.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            3030..3533
                     /gene="yeaK"
                     /locus_tag="ACHK74_004692"
     CDS             3030..3533
                     /gene="yeaK"
                     /locus_tag="ACHK74_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFP0223389.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3534..3638)
                     /gene="yoaI"
                     /locus_tag="ACHK74_004693"
     CDS             complement(3534..3638)
                     /gene="yoaI"
                     /locus_tag="ACHK74_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFP0223390.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3808..4254
                     /gene="yeaL"
                     /locus_tag="ACHK74_004694"
     CDS             3808..4254
                     /gene="yeaL"
                     /locus_tag="ACHK74_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFP0223391.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4211..5032)
                     /gene="nimR"
                     /locus_tag="ACHK74_004695"
     CDS             complement(4211..5032)
                     /gene="nimR"
                     /locus_tag="ACHK74_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFP0223392.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            5129..6310
                     /gene="nimT"
                     /locus_tag="ACHK74_004696"
     CDS             5129..6310
                     /gene="nimT"
                     /locus_tag="ACHK74_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFP0223393.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6365..6712
                     /gene="yeaO"
                     /locus_tag="ACHK74_004697"
     CDS             6365..6712
                     /gene="yeaO"
                     /locus_tag="ACHK74_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFP0223394.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6734..6988)
                     /gene="yoaF"
                     /locus_tag="ACHK74_004698"
     CDS             complement(6734..6988)
                     /gene="yoaF"
                     /locus_tag="ACHK74_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFP0223395.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRYHPAGEK"
     gene            7171..8196
                     /gene="dgcP"
                     /locus_tag="ACHK74_004699"
     CDS             7171..8196
                     /gene="dgcP"
                     /locus_tag="ACHK74_004699"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFP0223396.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMQEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            8229..8327
                     /gene="yoaK"
                     /locus_tag="ACHK74_004700"
     CDS             8229..8327
                     /gene="yoaK"
                     /locus_tag="ACHK74_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFP0223397.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8330..8404
                     /gene="yoaJ"
                     /locus_tag="ACHK74_004701"
     CDS             8330..8404
                     /gene="yoaJ"
                     /locus_tag="ACHK74_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFP0223398.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8463..8711)
                     /gene="yeaQ"
                     /locus_tag="ACHK74_004702"
     CDS             complement(8463..8711)
                     /gene="yeaQ"
                     /locus_tag="ACHK74_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP0223399.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8939..>9099)
                     /locus_tag="ACHK74_004703"
     CDS             complement(8939..>9099)
                     /locus_tag="ACHK74_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP0223400.1"
                     /translation="FRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEP
                     TEMIQATA"
BASE COUNT         2267 a         2180 c         2111 g         2541 t
ORIGIN      
        1 aagctcaaag cttttttatt ttcagtttat tgctgacgtt aattttattt tgcatcacca
       61 cattatataa cgaaaacaca aatgtaaaac tcatcccaca gatgaattac ctgatggttg
      121 ttgtggcttt gtttttcctt aacgccgtca tttttctttt catgttaatg aaatatttca
      181 ctaacaaaca aattttacca acactcattt taagccttgc atttttaagt ggccttatct
      241 atttagttga aaccattgta attatccata aaccaattaa cggcagtaca ctgatccaga
      301 caaagtcgaa tgatgtttct attttctata ttttccgcca actcagtttt atttgtttaa
      361 cctcgctggc actcttttgt tatggaaaag acaacatcct tgacaacaat aagaaaaaaa
      421 cgggaatcct gttgctggcg ctgattcctt ttttagtttt tccccttctg gcacacaatc
      481 tgagcagtta taacgctgac tattctttgt atgtcgtcga ctactgtccg gacaaccata
      541 ctgcgacctg gggaatcaac tatacaaaaa tattggtttg tctgtgggca tttttactgt
      601 tctttattat catgcgtaca cgattagcca gcgaactatg gccgttaata gcattattat
      661 gtctggcatc gctatgctgc aacttacttc tactgactct ggatgagtat aattacacca
      721 tctggtatat cagtcgcggg attgaagttt ccagtaaact gtttgttgtg tcttttctga
      781 tttataacat ttttcaggaa ctgcaactct ccagcaaact ggcagttcat gatgtgctga
      841 ccaatattta taatcggcgc tactttttca acagcgtaga gtcattattg tcgcgacctg
      901 ttgttaagga cttctgtgtc atgctggttg atattaatca gttcaaacgc atcaatgccc
      961 aatggggaca tcgtgtgggt gataaagtgc tggtttcaat tgtcgatatt atccagcaaa
     1021 gcatccgccc tgatgatatt ttagcgcgac tggagggtga ggtgtttggc ttgctattta
     1081 ccgaactcaa tagtgcccag gcaaaaatca ttgcggaacg tatgcgtaaa aatgtcgaac
     1141 tcctgaccgg ctttagtaac agatatgatg ttcctgaaca aatgaccatc agtattggca
     1201 cgtttttttc aacgggtgac acgcgtaata tctcgcttgt catgacggaa gcagataaag
     1261 ccttacgcga agcgaaaagc gaggggggca acaaagtgat tattcatcat atttaagtgc
     1321 aaaaatattc agagccatgc tttttgcgtg gcttttgcat acaatttatt actattaccc
     1381 ctaaatttcc cctccccagc ggcgcagaga tgagtataat tagcgcccct gtgccaggcc
     1441 gcaatcgaac tttatctggt tttctcgttt cactaaccga aggagtgcca tttatcatga
     1501 aattgcacca tagaatgctc cggcatttta tcgccgcaag tgtcattgtg ctgacatctt
     1561 ccttccttat ttttgaactt gtcgccagcg acagagcaat gagtgcctat ctgcgctata
     1621 tcgtgcagaa agcagactcc tcctttcttt atgataagta tcagaatcag agtattgccg
     1681 cgcatgtgat gcgcgctctc gctgctgagc agtcggaagt gtcgccagaa cagcggcgcg
     1741 ccatctgcga ggcttttgag tctgccaata atacccatgg cttaaacctg actgcccata
     1801 aatacccggg cttacgcggc acactacaaa ccgcatccac tgactgcgac acaattgtgg
     1861 aagctgcagc actattaccc gcttttgatc aggcagtgga aggcaaccgc caccaggatg
     1921 attacggttc aggtcttggg atggccgaag agaaatttca ctattatctc gatctcaatg
     1981 accgctatgt ctatttttat gagccggtta atgttgaata ctttgcgatg aataactggt
     2041 ccttcctgca gtcaggaagt attggtatcg atcgcaaaga tattgaaaag gtatttaccg
     2101 ggcgtaccgt attgtcgagc atttaccagg atcagcgtac taaacagaac gtgatgagtt
     2161 tgctgacgcc ggtgtatgtt gcagggcagc taaaagggat tgtgctgctg gatattaaca
     2221 aaaacaattt gcggaatatc ttttacactc atgaccgccc tctcctctgg cgttttctca
     2281 atgtcacgct aaccgatacc gattcggggc gcgacattat catcaaccag agcgaagata
     2341 atctgttcca gtatgtcagt tacgtccatg acttaccggg cggcattcgt gtctcgttat
     2401 ccattgatat tctttacttt atcacgtctt cgtggaaaag cgttctgttc tggattttga
     2461 cggcgttaat tttgctgaat atggtccgga tgcacttccg tttataccaa aatgtgtcgc
     2521 gagaaaatat tagtgatgcg atgactggac tgtataatcg caaaatttta acccctgaac
     2581 tggagcagcg gttgcagaaa ctggtgcaat ccggttcttc ggtgatgttt attgcaattg
     2641 acatggacaa gttaaagcaa ataaatgaca ccctcggtca tcaggagggg gatttagcga
     2701 ttacgttatt agctcaggcg attaaacaat cgattcgtaa aagtgattat gccatccgac
     2761 tcggtggcga tgaattctgc atcattcttg tcgattcgac gccgcaaatt gcagcacaac
     2821 tgcctgaacg tatcgaaaaa cgtctgcaac atatcgcgcc gcagaaagaa atcggcttct
     2881 cttccggtat ttacgcgatg aaagaaaacg atacgttaca tgatgcgtat aaagcttccg
     2941 atgagcgttt atatgtcaat aagcagaaca aaaacagccg ttcatgataa ccttctgtgg
     3001 ttgtttgctt gtaatctcag gagcgtgaaa tgactgaaat ggctaaagga agcgtgacgc
     3061 atcagcggtt aatcgcttta ttatcacaag aaggtgctaa cttccgcgtc gttacccatg
     3121 aagcggtagg taaatgtgaa gcggtgtcag aaattcgtgg caccgctcta ggccagggtg
     3181 ctaaggcact ggtatgtaaa gtcaaaggca atggcgtaaa tcaacatgtt ctggcgatcc
     3241 tcgctgccga tcaacaggcc gatctgagcc aactcgccag ccatataggc ggattacgcg
     3301 cttcgttggc cagcccggcg gaagtcgatg aactcacagg ctgtgtcttc ggcgcgatcc
     3361 cccctttcag cttccatcca aaactcaaac tggttgccga ccctctactc ttcgaacgat
     3421 ttgatgaaat cgccttcaat gcagggatgc tggataaatc cgttattttg aaaaccacgg
     3481 attatttgcg cattgcgcaa ccagaactgc tcaatttccg ccgcactgcg taactagcct
     3541 gtccgttcaa taagcagaac ggacaaaacc agcacaacag caatggcaaa aaacgatgac
     3601 gtgataatca gtgtctcgac gaacatttga tcgttcatag catgcccccg ggagatagct
     3661 gttttcacgt tattgttagc gtgcacaaat ggcagtttga tgacagttcg cctgattttt
     3721 tatgcaaaaa acgtaaagat tttctacttc cttcctgcag caagcgtaaa gtaagcaggc
     3781 ttattatttt ttggcaagga aaccacgatg tttgatgtca ctttgctgat cctgctcggg
     3841 ttagctgcgc tgggctttat cagtcataac accactgtcg ccgtttcaat tctggtgttg
     3901 atcattgtcc gcgtcacacc gttaagcacc tttttcccgt ggattgaaaa acaggggcta
     3961 agtatcggga taatcattct gacgattggc gttatggccc ccatcgccag cgggacgcta
     4021 ccgccctcaa ccttgattca ctcgtttctg aactggaaat cgctggtggc aattgcagta
     4081 ggcgttattg tctcctggct gggtgggcgc ggcgtgacct taatgggcag ccagccgcaa
     4141 ctggtcgccg ggctgctggt aggcactgtt ttaggcgtgg cgctgtttcg cggcgtaccg
     4201 gtcggcccgc ttattgccgc gggtctggtt tcgctgattg tggggaaaca gtagttaatc
     4261 ccgcaatata tcggcctggc gtttgcccca ggcctttctt aaacatagta ataaaggccg
     4321 ttgttgaatc ataccccagc gtatgagcaa ctttctgtac agtatcgcct ttcactaatc
     4381 cctgaagtgc cataatcagc tgtaactgct gacgccactg gcgaaaactc aaccccgtct
     4441 cttttactat cagtcgcgca agattgcgtt cactcatcgc aaaaaagcca gcccattgcc
     4501 ctaatgaccc ccactcgacg ggccctttcg ccatcatctc caccatcgtg cggattttag
     4561 gatgagaaga aacgggcagg tgcagctgtt gttgtggctg ctgagggagt tcatcaaaca
     4621 ggacctgaat taatctgcgg gtcatcggct cagcacgctg agtcgtcgtc ctgtttgcca
     4681 gggttaaaat caattcccgg cataagggcg atattttcaa tgtgcaacat gttgtcggca
     4741 ttgtcactgc gctgggttcg ataaacagaa aacagagttc agcgttagct gtcacttgat
     4801 tgctatgttc caccccgccc ggtatccaaa ccgcatattg cggcggcacc atccacaaag
     4861 cattttccac cgtacaggta attgcaccat gtaatgctag tatcagctgt cctttgcggt
     4921 gctgatgtac aggactggtt aactcatccg ttccggcgtg aatacaaaat gcgaccgcgg
     4981 cttcatggtg tcggtcgggt tcatagccat tgagattcaa cctgtgcatc attttgtccg
     5041 aatttagcga taatttgtca ttttagcttg attcaacata acaataaaaa cggtaaggta
     5101 cagcctcgtt tgtaacaatg agaagcatat gacctgttca acttcattaa gcggcaaaaa
     5161 caggattgtc cttatcgctg gcattctgat gattgccact acattacgcg tcaccttcac
     5221 cggcgcagca ccgttactcg atacgattcg ttccgcttac tcgctgacga cagcgcaaac
     5281 cggcttatta accaccctgc cattattggc ctttgcgcta atctcacctt tggctgcccc
     5341 ggtggcgcga cgttttggta tggaacgtag cctgtttgcc gcgttacttt tgatctgtgc
     5401 aggtatcgca attcgttctc tcccttcgcc ttacttatta tttggcggta cagcggtcat
     5461 tggcggtggg attgcattag gcaatgtctt actgccagga ttaattaaac gcgatttccc
     5521 tcattccgtc gccagactta ccggcgcata ttccctgaca atgggagctg cagcggcact
     5581 gggatcggct atggtcgtgc cgctggcttt gaacggtttt ggctggcaag gcgcgttgtt
     5641 catgctgatg tgttttcctc tgctggctct ttttttatgg ctgccacagt ggcgaagcca
     5701 acaacatgca aatttgagta cctcgcgcgc cttacatact cggggtatct ggcgttcgcc
     5761 gcttgcctgg caggtcacat tgtttcttgg gatcaactca ctggtctatt acgtgattat
     5821 tggctggctt ccggcgatcc tcatcagtcg cggctatagc gaagcacagg cgggttcact
     5881 gcatggtttg ctgcaactag ccacagcagc tcccggtttg ctgatccctc ttttcttaca
     5941 tcatgtgaaa gatcagcgtg gtattgcagc gttcgttgcc ttgatgtgcg cagtgggcgc
     6001 tgttgggctc tgctttatgc cagcgcacgc gatcacctgg actctgcttt tcggttttgg
     6061 ttccggcgca acaatgatac tggggttgac gttcattggt cttcgggcaa gttctgcgca
     6121 tcaggcggcg gcactctcgg ggatggcaca atccgtcggg tatttgctgg cagcctgtgg
     6181 gccgccgctg atgggtaaaa tacacgatgc taacggtgac tggtccgtac cactgatggg
     6241 tgttgccata ctttcactac tgatggcgat tttcggactt tgcgccggga gagacaaaga
     6301 aattcgctaa tatccggtgc tatagtgacg tgacaaatca tgcgtgaaag ggagaacaaa
     6361 cacgatgaat attcagtgca aacgcgttta tgatccggct gaacagagcg atggttatcg
     6421 cgtactggtc gaccgcctct ggccgcgcgg tatcaaaaaa accgatttag cccttgatga
     6481 gtgggataaa gaaatcacgc cgtcaacgga actgcgcaaa gcctttcacg gcgaagtcgt
     6541 cgattttgca acctttcgcg agcaatatct tgcagaactg gcgcaacacg agcaagaagg
     6601 aaagcggctg gcggacatcg ccaaaaaaca gcccctgacc ctgctctacg cagcaaaaaa
     6661 caccacgcag aaccatgcgc tggtgctggc cgactggcta cgtagcttgt gattttagta
     6721 cagcatccgg cggttatttt tcaccagccg gatggtaccg tcgccacaat gcccattcat
     6781 caatcgtttc accgcccggt aatttgcaat tgttgctgac cccttgcgct gtctgcactg
     6841 gaatgagcgt cccgcccttc tgctggcaat agaccgacgc cggatttgcc ataccaatct
     6901 gcggcggttt aggtgcttct ggctgagaag gggttgaaca accagccagg accagcaagc
     6961 aaggcagaac aaaactgata attttcattt attgatctca catatttatc cagacttaga
     7021 gtatcgcggt atcgttttgt tttgcagcac tatttttatt acattcactc aaatcatatt
     7081 acgccttgtt tcatctttgt tgatgatgtt ttatcatgcc tgcaaagatt aaataatcag
     7141 catttacccg ccgtatcctg gagttgtttc gtgtcagatc agattatcgc ccgcgtctcg
     7201 caatcccttg ccaaagaaca gtcactagaa agtctggtcc gacagcttct ggagatgcag
     7261 gaaatggtca ctgatatgga atcaacctac ctgaccaaag tggatgtcga agcgcgcctg
     7321 cagcatatta tgtttgcccg taacagccag aaaatgcaca tcccggagaa ttttaccgtc
     7381 tcgtgggatt actcgttatg caaacgcgct attgatgaaa actgcttttt cagcgatgaa
     7441 gtccctgacc gttggggcga ctgtattgcg gcacgcaatc ttggaatcac cacatttctg
     7501 agtacaccaa ttcacttacc ggatggatca ttctatggca cgctttgcgc cgccagcagt
     7561 gagaagcgcc agtggagtga acgcgcggaa caggttttac agttattcgc cggactgatt
     7621 gcacaatata ttcaaaaaga ggcgttggtt gaacagctgc gcgaagccaa tgccgcgctg
     7681 attgcgcaat cgtataccga ctcgttaacc gggctaccga atcggcgggc gatttttgaa
     7741 aatctgacga cgctgttttc tctcgcccgg catcttaacc ataagataat gatcgcgttt
     7801 atcgatctgg ataacttcaa attaatcaat gatcgttttg gtcataatag tggcgatctg
     7861 tttctcattc aggttggcga gcgccttaat acgctccagc aaaatggcga agttattggt
     7921 cgtctcggcg gtgatgagtt tttggttgtt tcactaaata acgagaatgc ggatatttcg
     7981 tcgctgcgag aacgtattca acagcaaata cgtggagaat atcacttagg tgatgttgat
     8041 ttgtattatc ccggtgccag tcttggcata gtagaagtcg atcctgaaac aaccgatgca
     8101 gacagtgccc tgcatgctgc cgatattgcg atgtatcagg agaaaaaaca caaacagaaa
     8161 acaccttttg tcgcgcatcc agcgctacat tcctgaggcg tattcacatc cttttgattg
     8221 gtgataacat gcgaatcggt attatttttc cggttgtaat ctttattaca gcggtcgtat
     8281 ttttagcatg gttttttatt ggcggctatg ctgccccggg agcataaaga tgaaaaaaac
     8341 aacgattatt atgatgggtg tggcgattat tgtcgtactc ggcactgagc tgggatggtg
     8401 gtaacgtcac ctctaaaaaa tagcaaaggc tgcctgtgtg cagcctttgt gcaatttaag
     8461 cgttaacttt taatcttcct gtagataaat agcacgacaa tcgcaccaat aacggcaacc
     8521 acgaagctgc caaaattgaa gccatcgact ttaccaaagc caaacagcgt gctgatccat
     8581 ccgccaacta cggcaccgac tatccccagc aggatagtca taaagaatcc accaccatct
     8641 ttacctggca tgatccactt cgccagaata ccggcaataa gcccaaaaat aatccatgac
     8701 agaatgccca ttgtttcctc acttatctgt tttgcattag cgggttagtc gctgataaaa
     8761 agcatagcac aacatcggga gggcaagatt tgtgacgagc atcacggagg atttttttgc
     8821 ggatggcaca gaaattgcgc catcaacgat cagtgataat taccaaccac aaacatcatg
     8881 ttcgttttcc gtgtcttgac atcctccacg ccctgaatga cgaggatccc tgctacgttt
     8941 aggctgtcgc ctgaatcatt tcggtgggtt cctgcttcaa cgagcggcct gactgcacca
     9001 tctctccaca ggcaagaacg gcgtgccccg ccgctaaaat gttacgagcg ccgtttacat
     9061 cggcgttcgc tgtatatcca catacctggc atctgaatt
//
LOCUS       DAZGSP010000158         8531 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZGSP010000158 DAZGSP010000000
VERSION     DAZGSP010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.159"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..688
                     /locus_tag="ACHK74_004704"
     CDS             <1..688
                     /locus_tag="ACHK74_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFP0223401.1"
                     /translation="VKLVMQLKILNIVNKNFIENMKKTFSHCPYIKDPIIRSYIHSGE
                     DNKFDDFMRQHRFSKVDFDTQQMIHFINRFNMNKGLIDKNNNFFIQLIDQALRSTDDM
                     IKANAWYLYKEWIRSDDVSPIFIETEEKLRTFNTNKLTRNDNIFILFSSVDDGPVMVV
                     SSQRLHDMLNPTKDTNWNSTYIYKSRHEMLPVNLTQETLFSSKSHGKYALFPIFTASW
                     RAHRIMNKGV"
     gene            911..1408
                     /gene="ubiC"
                     /locus_tag="ACHK74_004705"
     CDS             911..1408
                     /gene="ubiC"
                     /locus_tag="ACHK74_004705"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFP0223402.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1421..2293
                     /gene="ubiA"
                     /locus_tag="ACHK74_004706"
     CDS             1421..2293
                     /gene="ubiA"
                     /locus_tag="ACHK74_004706"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFP0223403.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2448..4871)
                     /gene="plsB"
                     /locus_tag="ACHK74_004707"
     CDS             complement(2448..4871)
                     /gene="plsB"
                     /locus_tag="ACHK74_004707"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFP0223404.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            5042..5410
                     /gene="dgkA"
                     /locus_tag="ACHK74_004708"
     CDS             5042..5410
                     /gene="dgkA"
                     /locus_tag="ACHK74_004708"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP0223405.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5520..6128
                     /gene="lexA"
                     /locus_tag="ACHK74_004709"
     CDS             5520..6128
                     /gene="lexA"
                     /locus_tag="ACHK74_004709"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFP0223406.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6201..7526
                     /gene="dinF"
                     /locus_tag="ACHK74_004710"
     CDS             6201..7526
                     /gene="dinF"
                     /locus_tag="ACHK74_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFP0223407.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEILKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQELADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7642..7851
                     /gene="yjbJ"
                     /locus_tag="ACHK74_004711"
     CDS             7642..7851
                     /gene="yjbJ"
                     /locus_tag="ACHK74_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP0223408.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7893..8408)
                     /gene="zur"
                     /locus_tag="ACHK74_004712"
     CDS             complement(7893..8408)
                     /gene="zur"
                     /locus_tag="ACHK74_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFP0223409.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1976 a         2050 c         2298 g         2207 t
ORIGIN      
        1 agtaaaactg gttatgcaat taaaaattct taatattgtt aataaaaact ttattgagaa
       61 tatgaagaaa acatttagcc attgtcctta tataaaagat ccaattatac gcagttatat
      121 ccattctggt gaagataaca agttcgatga ttttatgcgt caacatcgat tcagcaaggt
      181 ggatttcgat acccaacaga tgatccattt tattaacagg tttaatatga ataaagggct
      241 gattgataaa aataacaatt tttttatcca acttatcgat caggccttac gatcaacgga
      301 tgatatgatc aaagcaaatg cctggtatct ttataaagag tggattcgta gtgatgatgt
      361 ttcacctata tttatagaaa ctgaagaaaa gttaagaacc tttaacacta ataaattaac
      421 acgaaacgat aatatattta tcctgttctc ctctgtcgat gatgggcctg ttatggtggt
      481 aagctcccag cgcttacatg atatgttgaa tcctacaaaa gataccaatt ggaattccac
      541 gtatatctac aaatccagac atgagatgtt gcctgttaat cttactcagg aaacactttt
      601 cagctccaaa tctcatggta aatatgcgct tttccccatt tttactgcga gttggcgagc
      661 tcatcgtata atgaataagg gtgtttaagt aaaggaaaac atcaccgttc ctggcatcct
      721 gggcggtgat gcccctatgg ttgccctctc cagcgcgggc atcggcaaag cgtaaggttc
      781 aacagcgttt taccacttca tgcgattgtt gcgtttttgt tgcgtattag atcacttaat
      841 ttgctttaca tctcccgtaa acacttttct gcgatacaat gcctttacgt tatgtaacgg
      901 agagttcggc atgtcacacc ccgcgttaac gcaactgcgt gcgctgcgct attttaaaga
      961 gatccctgcc ctggaaccgc aactgctcga ctggctgttg ctggaggatt ccatgacaaa
     1021 acgttttgaa cagcagggaa aaacggtaag cgtgacgatg atccgcgaag ggtttgtcga
     1081 gcagaatgaa atccccgaag aactgccgct gctgccgaaa gagtctcgtt actggttacg
     1141 tgaaattttg ttatgtgccg atggtgaacc gtggcttgcc ggtcgtaccg tcgttcctgt
     1201 gtcaacgtta agcgggccgg agctggcgtt acaaaaattg ggtaaaacgc cgttaggacg
     1261 ctatctgttc acatcatcga cattaacccg ggactttatt gagataggcc gtgatgccgg
     1321 actgtggggg cgacgttccc gcctgcgatt aagcggtaaa ccgctgttgc taacagaact
     1381 gtttttaccg gcgtcaccgt tgtactaaga ggaaaaaaat atggagtgga gtctgacgca
     1441 gaataagctg ctggcgtttc atcgcttaat gcgtacggat aagccaattg gcgcgttact
     1501 gctgctctgg ccaacattat gggcgttgtg ggtggcgaca ccgggcgttc ctcagctctg
     1561 gatcctggcg gtatttgtcg cgggtgtctg gctgatgcgc gctgctggat gtgtggtgaa
     1621 tgattatgct gaccgaaagt ttgatggtca tgttaagcgc acggcgaacc gaccacttcc
     1681 cagcggcgcg gtaacagaga aagaggcgcg cgcgctgttt gtagtgctgg tactgatttc
     1741 gtttttactg gtactgacgc tgaatacgat gaccattctg ttgtcgattg ccgctctggc
     1801 gttggcgtgg gtgtacccgt ttatgaagcg gtatacccat ctcccgcaag tggtgctggg
     1861 cgcggcgttt ggctggtcga ttccaatggc ttttgccgct gtgagtgagt cggtgccact
     1921 gagttgctgg ttaatgttcc tcgccaatat tctctgggcg gtggcttacg acacgcagta
     1981 tgcgatggtt gaccgcgatg atgatgtgaa gattggcatt aaatccacgg caatcctgtt
     2041 cggccaatac gataaattga ttattggtat tttgcagatt ggcgtactgg cactgatggc
     2101 gatcatcggt gagttaaatg gcttaggctg gggatattac tggtcaattg tggtggctgg
     2161 cgcgctgttt gtttatcaac aaaaactgat tgccaaccgc gagcgtgaag cctgctttaa
     2221 agcatttatg aataataact atgttggtct ggtactgttt ttaggactgg caatgagtta
     2281 ctggcatttc tgatgatgta aaaaagccgg atgatcatcc ggctttcttc tgggttgcct
     2341 tatgcgcggc gcttctcagg cctacacaac acatcgcaat ttattgaatt tgcagattat
     2401 ggaaggccgg ataaggcgtt ttcgccgcat ccggcaattc tctctgatta cccttcgccc
     2461 tgcgtcgcac tctcaatcgt caaacgcacg tctgatgtaa tcaactccgc cagcaactga
     2521 taaaccttca tcgtttctgc cggttcggcg tcgccgctat cgctgatata cccttcatca
     2581 cgcagcgtca gcaccagaga actgaacacc gccttgtcga agaactccgg cgcgttgatg
     2641 ccgtgcagca cggagagacg ttgcgcgacg gtgcggctct ctttctccag cgtaccgcgg
     2701 ttgatcgacg ggttggcact caacaaccag aaggtgatgg cataacgttg cagcgtttcg
     2761 cgcgcgcctg cggccagcag ctgtagggtg cgagaatgcg ccgggttgat atgcaactca
     2821 tcatcttgca gggtaatcag cccctgacgt tgcatctcat ttgccagcgc atcaataacg
     2881 tccggcaact cgtcgcgatc ccagcgcagg aacagctccg ctttcagcat tgggtaaagc
     2941 acattgacgt gctccatcaa tacgtcgcgg gagatgtggc gatgctgggt gacaattgcc
     3001 gccatcagcg aaggcagcac caacatatgc gcaatgttgt tgcgatagta ggtcatcagc
     3061 accgcttgct cgcgtggcag aatgatgatg tcgccgatag tgtctttctc gacttcaaac
     3121 ttgttcattt gcagcgcgtg atcgataagc tcgctggcgc tggctgaagg aacggtagag
     3181 tccgtggagt aaggtacgtt gcgcatcaga tccaggtagc agttgagttg ctcggttaac
     3241 tgctcgcggg tgagtgagcg ctgacgtgat gccagtagcg cagtacagca caggttcatg
     3301 gcgtttgccg cgcctgcgtt gttaatgcgt accatcagat cggcagcaat attattgacc
     3361 gtcggcgtta accaggccgg acgcaccgct tcgatgggat cgatagattc acgccagtct
     3421 ggtacgtgct ggttaaggta ggtcatcaac ggcattggtt caccgaagtt gacgtaaccc
     3481 tgaccgagat tacgcagctt gcttaaaccg cgcagcatct gcggcaggct ctctttctct
     3541 ttcgtcgcgc cgcgcagttc tttggcgtaa gtacccactt ccatgacgtg ctcataaccg
     3601 atatagatcg gaatcagcgt aatcggacgc gtgccgccac gcagcatcgc ctgaatggtc
     3661 atcgacagcg taccggtttt cggatccagc aaacgccccg tgcgggaacg accgccttcc
     3721 acgaagtact cgacggaata accacggctg aacagttcgc cgagatactc acggaaaacg
     3781 gtggaataaa gtttattgcc tttaaaagta cggcgaataa agaacgcccc cagacggcgg
     3841 aaaatcggcc cggccggcca gaagttcagg ttgatcccgg cggcgatatg cggcggcacc
     3901 agcccctggt ggtacagcac gtaagaaagt agcaggtagt ccatgtgact gcggtggcaa
     3961 ggcacataca ccagctcatg accgtcgtgg gccagctggc gaacgcgctc ggcgttatgg
     4021 acgttgatgc cctgataaag tcggttccag gtgaagccca gaatgcggtc agtcaggcgg
     4081 atcatctcgt aagagaaatt cgccgcaatc tcttccatca gcgcaatcgc gttctgctgc
     4141 gctttttcat gggagatttt tttgctgcgc gcttcatctt ctaccgcttt ggcaatggcg
     4201 cgggaggcga gcagcttatt aaacagatcc tgacgagcag gcagacgtgg gcccacagca
     4261 gccagacgtt gacgggcaaa gtgcatacgc gccacgcgcg ccagtttctg agcgatagtt
     4321 ttatctgtgc cgtgttcatc ggccatgcgg cgcagagaaa ctgacggcga gaaacgcaca
     4381 aaactgtcgc gaccgagcca cagtacggcg aaaaattttt gtacgccgtt aagcatacgc
     4441 agcggcgggt ttacttcgcc tttttcacgc cccggcgcgc gaccaaacat caccgatact
     4501 ggcaccatct gcacatccag atttgggttg ctgcggtgca aatcaagata gtcgtggaac
     4561 agcttaatag actcttcttt cggcgtgtaa taggtgaaca cacgcggccc gccgtgaatg
     4621 aacacatagc gcggcagtag cgtgccgtcg atttccagcg gctctaacgg gtcaggcaag
     4681 tcatgagcca gacactgggc gcgcaacgtc agcaaatctg ctttcgagtt gtacggtaaa
     4741 acgtacataa ttggacgaga ggtatccagc cccagttccg gggcaggatc tgccggaata
     4801 gacttgcttt ttaccaggat gcttaatggt aaattcagta atttgtagta aattcgtggc
     4861 cagccggaca taaacgatgt aaagcctctg gttaataatg caaatgcgcg gcaaggatag
     4921 cagaaagtca tgggaaattc tgtggtatcc gctcatgttt cgcgcggcgt tacgcaaacc
     4981 cgaatcatcg gatttaacgg tacactgata ttgacgctca taatgtaaaa aggttctttc
     5041 aatggccaat aataccactg gattcacccg aattatcaaa gctgctggct attcctggaa
     5101 aggtttacgc gctgcatgga tcaacgaagc ggcattccgt caggaaggcg tagcggtatt
     5161 gttggcggtg gtcatcgcct gctggctgga tgtggacgca attacccgcg tgctgcttat
     5221 cagctccgtg atgctggtga tgattgtgga aatcctcaat agcgccatcg aagcagtggt
     5281 tgaccgaatt ggctctgaat accatgagct ttccggacgc gcaaaagata tgggatccgc
     5341 cgcggtgctg attgccatta tcgtcgccgt gattacctgg tgcatcctgt tatggtcgca
     5401 ttttggataa cccttccaga tttcgataaa tctctggttt attgtgcagt ttatggttcc
     5461 aaaatcgcct tttgctgtat atactcacag cataactgta tatacaccca gggggcggaa
     5521 tgaaagcgtt aacggccagg caacaagagg tgtttgatct catccgtgat cacatcagcc
     5581 agacaggtat gccgccgacg cgtgcggaaa tcgcgcagcg tttggggttc cgttccccaa
     5641 acgcggctga agaacatctg aaggcgctgg cacgcaaagg cgttattgaa attgtttccg
     5701 gcgcatcacg cgggattcgt ctgttgcagg aagaggaaga agggttgccg ctggtaggtc
     5761 gtgtggctgc cggtgaaccg cttctggcgc aacagcatat tgaaggtcat tatcaggtcg
     5821 atccttcctt gttcaagccg aatgctgatt tcctgctgcg cgttagcggg atgtcgatga
     5881 aagatatcgg cattatggat ggtgacttgc tggcagtgca taaaactcag gatgtacgta
     5941 acggtcaggt cgttgtcgca cgtattgatg acgaagttac cgttaagcgc ctgaaaaaac
     6001 agggcaataa agtcgaactg ttgccagaaa atagcgagtt taaaccaatt gtcgttgacc
     6061 ttcgtcagca gagcttcacc attgaagggc tggcggttgg ggttattcgc aacggcgact
     6121 ggctgtaaca tatctctgag accgcgatgc cgccaggcgt cgcggtttgt ttttcatctc
     6181 tcttcatcag gcttgtctgc atggcattcc tcacttcatc tgataaagca ctctggcatc
     6241 tcgccttacc catgattttc tccaatatca ccgttccgtt gctgggactg gtcgatacgg
     6301 cggtaattgg tcatcttgat agcccggttt atttgggcgg cgtggcggtt ggtgcaacgg
     6361 cgaccagctt tctctttatg ctgttgctgt ttttacgcat gagcaccacc gggctgactg
     6421 cgcaggctta tggtgccaaa aatcctcagg cattagcccg tgcgctggtg caaccgttgc
     6481 tgttggcatt gggggctggg gcgttaattg cgctgctgcg tacacctatt atcgatctgg
     6541 cgctgcatat tgttggcggt agtgaagcag tcctggaaca ggcgcggcgc tttcttgaaa
     6601 tccgctggtt aagcgcaccg gcgtcgctgg cgaatctggt attactcggt tggttactcg
     6661 gcgtgcaata tgcccgtgcg ccagtaattt tgttagtggt cggcaatatc ctcaacattg
     6721 tgctggatgt ctggctggtg atggggctgc atatgaacgt gcagggcgcg gcgctggcga
     6781 cggttattgc ggaatatgca acattgctga ttggtctgct aatggtgcgt aaaatcctca
     6841 aactacgcgg aatttccggc gaaatactga aaactgcctg gcgaggaaac ttccgtcgct
     6901 tgctggcgct taaccgcgat atcatgctgc gttcgctgtt gttgcaactc tgtttcggcg
     6961 cgatcaccgt acttggtgcg cgactgggga gtgacattat cgctgttaac gcggttctga
     7021 tgacgctact cacctttacc gcctatgcgc tggatggttt tgcctacgcg gttgaagcac
     7081 attccggtca ggcatacggt gcgcgcgacg gtagccagtt gctggatgtc tggcgggcag
     7141 cgtgccgcca gtcggggatc gtagcgctac tgttttcggt ggtttatttg ctggcagggg
     7201 aacacatcat tgcgttactg acgtcgttaa cccagattca ggagctggcc gaccgctatc
     7261 ttatctggca ggtgattttg ccgttggttg gcgtctggtg ttatctgctg gacggcatgt
     7321 ttataggcgc aacgcgtgcc gccgaaatgc gtaacagtat ggcggtggcc gccgcaggtt
     7381 ttgcgctgac gctccttacg ctgccgtggc tgggtaatca tggtttgtgg ctggcattaa
     7441 ccgtctttct ggcgttgcgc gggctttctc tggcggctat ctggcggcgt cactggcgca
     7501 acggtacctg gtttgccgca acgtgacggt taaaaattct gaataaataa tcctaagcca
     7561 aattgctgac tacacttaat ctcacgttca gaagaaaagt gaacgtactc tcattcacaa
     7621 cctaacgatg aggtcttgat tatgaataaa gatgaagccg gcggtaactg gaaacagttt
     7681 aaaggtaaag tgaaagagca atggggcaaa ctgaccgatg atgatatgac gatcattgaa
     7741 ggtaaacgtg atcaactggt cggtaaaatc caggaacgtt atggttatca gaaagatcag
     7801 gcagaaaaag aggtcgtgga ttgggaaacc cgcaatgaat atcgctggta attaatccct
     7861 cctgcccgac gtgtacaagg atgtacgccc tcttaacgcg gtttcttttt cacctgcaca
     7921 gagtgatcat gctggcactg ttcaggatga cgacacgctt ccacttccac gcatgccgca
     7981 cagagcccat gtgcttcaat cacattatgc cgcagggcaa accccatttt tgccgccagc
     8041 gtatgcataa tgtcttccac gccttctgca cactcttctt tcaccgcgcc gcagcgatcg
     8101 catataaaca tggctgacgt atgcgtgggc tggtcgaaca gatgacagag cacataactg
     8161 ttggtggatt ccaccttatg cacaaaacct tgctcaagca gaaaatccag cgcgcgataa
     8221 accgttggcg gcttggcttg cggttcagct tcgcgcagta aatcaagcag atcataagcg
     8281 ctgatagcgc catcctgcag actcatcagg cgcaacactt ccaggcgctg tggggtcagg
     8341 cgcacattac gctgcgcgca gattttttca gcctgcgcta ataactcctg cgttgtggtc
     8401 ttttccatcg ggcacctcaa agtgggggtt aagaatgtcc ttactttacc atgttccagg
     8461 aaaaacaccg agaaccctta ttgttgccgt aatgttgatt ttctgttttg taggtaaggt
     8521 gttgtgttgc c
//
LOCUS       DAZGSP010000159         8518 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZGSP010000159 DAZGSP010000000
VERSION     DAZGSP010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.160"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..779
                     /locus_tag="ACHK74_004713"
     CDS             <1..779
                     /locus_tag="ACHK74_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP0223410.1"
                     /translation="SRFMQSLQQVLNKNEFQLLLGCHEYNQLKEAEILMTLLQGNPAA
                     LVIFGSQLADKTHQILEKTNIPTINVVGSPFSPAQITIETAFFEASHKLTEHMLEQGY
                     KNIGFIGAHMDNRLQRQQLNGWHKAMLSYYQNSDLVITMPGAASLQLGRYALNEILLR
                     QPELDAVICSHEEIALGIMFECQRRLLKIPGNIAVACLDGSDSCDQTHPTLTSIRIDY
                     KKMGTETGKLLIGLLNNNHDESEESRIVQFNYQIELRQST"
     gene            982..1662
                     /locus_tag="ACHK74_004714"
                     /pseudo
     CDS             982..1662
                     /locus_tag="ACHK74_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803817.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1679..1987
                     /locus_tag="ACHK74_004715"
                     /pseudo
     CDS             1679..1987
                     /locus_tag="ACHK74_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(2090..3196)
                     /locus_tag="ACHK74_004716"
                     /pseudo
     CDS             complement(2090..3196)
                     /locus_tag="ACHK74_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3962..6010
                     /gene="ireA"
                     /locus_tag="ACHK74_004717"
     CDS             3962..6010
                     /gene="ireA"
                     /locus_tag="ACHK74_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HFP0223411.1"
                     /translation="MKNKYIIAPGIAVICSTVISSGYASSDKKEDTLVVTASGFTQQL
                     RNAPASVSVITSEQLQKKPVSDLVDAVKDVEGISITGGNEKPDISIRGLSGDYTLILV
                     DGRRQSGRESRPNGSGGFEAGFIPPVEAIERIEVIRGPMSSLYGSDAIGGVINIITKP
                     VNNQTWDGVLGLGGIIQEHGKFGNSTTNDFYLSGPLVKDKLGLQLYGGMNYRKEDSIS
                     QGTPAKDNKNITATLQFTPTESQKFVFEYGKNNQEHTLTPGDSLDAWTMRGNLKQPNS
                     KRETHNSRSHWVAAWNAQGEILHPEIAVYQEKVIREVKSGKKDKYNHWDLNYESRKPE
                     ITNTIIDAKVTAFLPENVLTIGGQFQHAELRDDSATGKKTTETQSVSVKQKAVFIENE
                     YAATDSLALTGGLRLDNHEIYGSYWNPRLYAVYNLTDNLTLKGGIAKAFRAPSIREVS
                     PGFGTLTQGGASIMYGNRDLKPETSVTEEIGIIYNNDSGFSASATLFNTDFKNKLTSY
                     DIGTKDPVTGLNTFIYDNVGEANIRGVELATQIPVYDKWRVSANYTFTDSRRESDDES
                     LNGKSLKGEPLERTPRHAANAKLEWDYTQDITFYSSLNYTGKQIWAAQRNGAKVPRVR
                     NGFTSMDIGMNYQFLPGTLINFAVLNVTDRKSEDIDTIDGNWQVDEGRRYWANVRISF
                     "
     gene            6684..>8518
                     /locus_tag="ACHK74_004718"
     CDS             6684..>8518
                     /locus_tag="ACHK74_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP0223412.1"
                     /translation="MNLRKKHICIAISLVLNPASISQVSAQADTNKLTVEVDETSLGH
                     DVYGDDWKSIEIGTTKFTAKDESKGINFDHKSPVAEGRNSIAIGTSAEAGGDISTKEG
                     LYTGESIAIGAKTKATKEQSIAIGGDTKATGWGAIVIGGDDLTPLHGKTYGREKIENN
                     YLASEAKGDGSIALGSRTFAKGELSVSLGSVSSAEGMASNAIGATAEAKGDMATAVGF
                     NALANAGNALAVGSQSSANKNDALAVGSEANAAQNETVALGHGATVTVAKGNVSIGAD
                     SRDKQAQKVTEAKVRTKRGAEVTYSGFAGNNAASVVSVGDAGKERQIVNVGAGAVSEK
                     STDAINGSQLYAIAKTLKDDLDAINPTWTLKDDKGGSATINGGKDNKNGNTLTVKGDG
                     DIAVTVNDKGMTIDGTTLKNEIKNDAIKIKGGDGKTLTKSLGDNVEITGKGPIKTQVA
                     GDKLEISAETASLPVNGGNGKITVNNGDGDKLVTAKNIADAINQSGWKIKADKDTGGV
                     IEGNGQGTETLIKPGDMVTLKAGKNLSVKQSNGDFTFSVIDTPSFSSVQLNGKGNNGS
                     VTLSSEGNKLTLSGGNGAGQNGVVISGVASGLEGKELSTLTNTDK"
BASE COUNT         2489 a         1750 c         2022 g         2257 t
ORIGIN      
        1 gctcccgctt tatgcagtcg ttgcagcagg tgcttaataa aaatgaattc cagctactgc
       61 ttgggtgtca tgagtataat cagctcaaag aagctgaaat cctgatgacg cttcttcaag
      121 gtaacccggc ggcgttagtt atttttggtt ctcagttggc tgataaaact caccagatac
      181 tcgagaaaac aaatatacct accattaacg ttgtcggttc ccctttcagt ccggctcaaa
      241 ttaccatcga aacagcattt tttgaagcat cacataaact gaccgaacat atgctggaac
      301 agggttataa gaatatcgga tttatcggtg cgcatatgga caaccgcctg caacgtcagc
      361 aactgaacgg ctggcataaa gcaatgctga gctattacca gaactctgat cttgttatca
      421 ccatgccggg tgccgcaagt ctgcaattgg ggcgttacgc attaaacgag atattactgc
      481 gacaaccaga gctggatgct gtgatttgca gccacgaaga gattgcgctg ggtatcatgt
      541 ttgagtgcca gcggcgtttg ttaaaaatcc ctggaaatat cgctgtagct tgtcttgacg
      601 gttcagacag ttgcgatcaa acacacccaa ccttgacatc catccgtatt gactacaaaa
      661 aaatggggac agagacgggt aagctgctga ttggactatt gaataataat catgatgaat
      721 cagaagagtc gcgtatcgtg caattcaact accagataga attacgtcag agtacatgag
      781 ctcaaaaggg gattattgca aagttagcta ttgtggtagg gatagccgtt tccggctatt
      841 ccccgcgcag agccggatgt gtgctgctaa cgcatcctgt caacgacgga tgaaaagtta
      901 tccacttata tctccaccaa cggcccaata ttgtaccgtt cgtgacacat ccatttctac
      961 ttcaggtatt tggcgtagta gcgggtgcta aaaatttgcc gcaaaaccgc aatatcagcc
     1021 cgcagaggcg ctctccagat tatccattcc tatattatct ctgcaccgga tgaattactc
     1081 gaacaactca gaaatatgac acatatgcaa cttatcagga ctctgggatc ctggaggcct
     1141 gatgccagtg aatacagtaa tgttaccaac gtttatcgca ttacattgaa atcccttgtt
     1201 cgacgctatt tcgagtttca tgatgaaatc accaacttgg atgtcatgat tgcggcaatt
     1261 gttgatgaac tggcacctga actgattaaa cgtaatgctg ttggatacga aagcgctttg
     1321 cagttgttga tctctgccgg agacaatccc taacggttaa gaaccgaatc aggttttgcg
     1381 gcgttgtgcg gtgtcagcca gtttccccta tcttctggaa aaacgaacca ttaccgactt
     1441 aatcggggtg gggagcgtgt tgcaaatagt gcactttaca tcatcactat aggatgttta
     1501 caaactgacg ataaaacaaa ggaatatgtc tccaggcgag tagccgaagg acatacaaaa
     1561 atggaagcaa tacgctgtct gaagcgctat atctcacgcg aagtttatac attactacga
     1621 aatcaaaaca ggcatgttaa ctgcatcccg ataacgtctt gactcttaga agggcgccca
     1681 aggcagtcat tattcgagca ggcagttccg gcagttattg tgtcgatacc agatcaggca
     1741 gagtatgagc cggcgcggaa aatgctggga taacagctca atggaacgct tcttcaggag
     1801 tctgaagaac gaatggatgc cggtggtggg ttacgtaagc ttcagcgagg cagctcactc
     1861 cataacggac gatatcgttg gatattacag cgcactaaga ccgcacgaat ataacagtgg
     1921 gttatcccca aacgaatcgg aaaatcgata ctggaaaaac tctaactcgg tggccagttt
     1981 ttgttgacca cttcagccat cgccagacgc taacatgagt tactcttgtt ctgagctacg
     2041 tcaccttcag ggaactggtt atcctcattc tttatttgtg acattgtggt tattgtgcga
     2101 caaacgtggc tatcccgccc catatcccag ataaaaccac ctgctcacgt gcgacagcga
     2161 caattgtaat attgacattc tttcctttgg cctgaagttt tcgagacctc ttgcaaagcc
     2221 tgagttgagc atcccatgct ctgtcaatga cggggcgggg taaattttcc tgattttttg
     2281 tattgccgga ctgatgcgtg tcggatgccg gtaactccac gctgcctcaa ccaatagctt
     2341 tctggcatag ctgtttccgg ctttggtaat gctgccctga tgtctgcttc cacccgatga
     2401 atattctgat ggcgtgatac caaaccatga catcagttgt cgtggatgtt caaaacgggt
     2461 cagatcaccc agttcagaaa ttaatccaac cgcagtaata aactgaatcc ctcgcatggc
     2521 ttgctgcgcc tctactaccg gatacagcct ccattcggta acggcctcct tcgggcggat
     2581 tcaagccgtt cacattgtgc ctgccggtct tctattgtac aacggtgttc gtcaaaagct
     2641 aattgtcgcc agggactttc gaatgagtat ttgctgagcc agtgtcggtg tgcagggccc
     2701 caatctgctc gcccgacata atggacccca tgaaccagaa gaaacgattt cagacgttgc
     2761 cttgcatgcc gtaaatcatc gcgggcagat gcccatgcct gggccaaatc ccggaatgcc
     2821 tcatcttcaa cgccggatac gtagacagca gaaagatctc ctgcacgcag tgaacgcaca
     2881 agtctgatgg catcacggtg tcggttttac cccgctcccc cggttttttc gggataagtg
     2941 agggggcgca gaccatgcag tcaaaaccgg atttcaccag ccgacgataa agtccataac
     3001 cacagggtcc cgctttataa acgatactga cctgcgatga ctttgacctg atacgttgat
     3061 aaagattatg aatgtcagta ggtgatgtgc taattttacc catcagttca acaggttctg
     3121 aattgatagc ataggagaca gtaatcgatt ctttgtgaac atctaattcg acgtacaaca
     3181 tgttattgtt gttcatgctg gcctccgtgg tggaaattcc aggggggtag caacagctca
     3241 cccatgtggc tctgacaacg atgttaaccc gcattaaata tcccacgttg gacagcatct
     3301 gtctcacgga aagtcatact gccttacgtg catatttcac cttacgcctt cactgtctgt
     3361 catcggaagt actgatttat attggggctt tgcacttact gtcattcacg ggaaaagatg
     3421 gtggtgaaca gtacgatact gtcttggctg ttgccgaggg aagattcagg agtggtaagg
     3481 gggttagtgg tgtaagatga gtctggagct ctgactggtc agtgacgcga aactcaatct
     3541 gccaggataa actccccctt gcttttgcgc agaagcagtg ggccttggtt cgcttatgtc
     3601 ctgcatctac aagcgaatga ctgaccggag actcccatat ctggtcagtc gcccattttt
     3661 attttccacc acatcggaca agcataccgt aataaacagc accagtctcc tgttcactct
     3721 tgcttgattt actggaatgc tgtttatgga gatatccggt tgaaagattt tacctcttcg
     3781 acaggactat aaattataca gtaaggagtc cctcgtagaa aggggctgat tctcatcaag
     3841 tcggacaggt tcaaagtgtt acctgaatga gctaaatgtt atttaccttc ctttctgttc
     3901 attgtaaatt tttcttgcta acgcaaatca ttatcattac gattttagaa aagggaaatt
     3961 tatgaagaac aaatatatca ttgctccggg cattgccgtg atatgttcta cagtcatatc
     4021 atcaggttat gccagttctg ataaaaaaga agatacgctt gttgttactg cctccggctt
     4081 cactcagcaa ctcagaaatg caccggccag tgtttcagtc attacttcag agcaactgca
     4141 aaaaaaaccg gtttcagacc tggtcgatgc ggtaaaagat gttgaaggga ttagtatcac
     4201 cggtgggaat gaaaaaccgg atatcagtat acgtgggtta agtggcgatt atacattgat
     4261 tctggtcgat ggacgacgtc agagtggtcg ggaatccaga ccaaacggca gcggcggttt
     4321 tgaagccgga tttatccctc ctgtggaagc aattgagcgt attgaagtta tacgtggccc
     4381 tatgtcttcc ctgtatggtt ccgatgctat cggaggagtt attaatatca taaccaaacc
     4441 tgttaataac cagacatggg atggcgtact tgggcttggg ggaattattc aggaacatgg
     4501 gaaatttggt aactcaacca cgaatgactt ctacctgtca ggcccattgg ttaaggataa
     4561 acttggtctt cagttatatg gaggaatgaa ctatcgcaag gaagatagta tctctcaggg
     4621 aacaccagca aaagataata agaatataac ggcaacactc cagtttactc cgactgaaag
     4681 ccagaagttt gtttttgaat acggaaaaaa taaccaggaa catacattaa cacctggtga
     4741 ttctcttgat gcctggacta tgcggggaaa tcttaaacaa ccaaacagta aaagagaaac
     4801 acataactca cgtagtcact gggtagcagc atggaatgcc cagggcgaaa tactgcatcc
     4861 tgaaattgct gtttatcagg agaaagttat tcgtgaggtt aaatctggta aaaaagataa
     4921 atataatcat tgggatctta attacgagtc aagaaaaccg gaaataacca acacgatcat
     4981 agatgcaaaa gtgacggcat ttctgccgga aaatgtgctg accatcggag gtcaatttca
     5041 gcatgcagaa ctccgtgatg actcagccac aggtaaaaaa acgacagaaa cacagtctgt
     5101 ttcagttaaa cagaaagctg tttttataga aaatgaatat gctgcaacgg attctctcgc
     5161 cctgactgga gggctgcgcc tcgataacca tgaaatctat ggcagttact ggaatccaag
     5221 attgtatgct gtttataacc tgaccgataa tctcactctc aaaggaggga tcgcaaaagc
     5281 atttcgggcc ccttcgattc gtgaggtcag tcctggattt ggaacactga cgcagggtgg
     5341 tgcctctatt atgtatggaa acagggacct gaaaccggag accagtgtaa ccgaagagat
     5401 cggtattatt tataataatg acagtggttt ttcagcaagc gcgactctgt ttaatacaga
     5461 ttttaaaaat aagctgacca gttacgatat aggcacaaaa gatccagtca ctgggctaaa
     5521 cacttttatt tatgataatg tgggtgaggc aaatatcaga ggtgtggagc ttgcaactca
     5581 gattcctgtg tatgataaat ggcgtgtatc tgcaaactat acattcactg actcccgtcg
     5641 agaaagcgat gacgaaagtc tcaatggcaa gtcgctgaaa ggggaacctc tggaaagaac
     5701 tcccagacat gcagccaatg caaaactgga atgggattac actcaggata ttacatttta
     5761 ttcatctctg aattatacag gaaaacaaat ctgggcagca caaagaaatg gtgccaaggt
     5821 tccccgtgtt cgtaatggat tcacttctat ggatattggt atgaattacc agttcctgcc
     5881 aggcacgctg attaattttg ccgtacttaa cgtcacagac agaaagagcg aagatatcga
     5941 taccattgat ggtaactggc aggtcgatga aggacgtcgt tactgggcta atgtaagaat
     6001 atccttctga gagaatatag ataacctgag gccgtgcgcc tcaggtacca gtatagtaat
     6061 cagtaacgtc agcttccagg ttgtaatgct tttaattttg ataaaaataa acttaataaa
     6121 ttacatttgt taaatattga taattttaat ttggttttat tactccagtt atactcacac
     6181 tctatcttta cacaattcat caccattaca cctccatagc ctgttgattt aaaagattta
     6241 atttcctgtg aaacaatcag gcctgtatga acctgattat accacataat cttttaactt
     6301 atttaaattt aactctcagg cttttctgtc agtaaaatca gaaagaattg caggatttcg
     6361 tttttttgct gcgaagtgaa atgtatctca tttttactgc cttcgccagt gcgtcaggca
     6421 atggcaggta gtgtacctgc attctgctgt aacgcgtaat gtcgggtagt ttacttaaac
     6481 ttaatatgtt ttatgttcgc attattataa atcactttat tgccttttgt ctgcttttta
     6541 atggttcccg ttttgttcac ttctggcttt ataaaaaatg gatgctgaag aaatgaaggc
     6601 agtccacctt acataatatt catgcatgat ttttatgcta ttccgactga gtggaagaaa
     6661 ataaaggaga aaagagggta tatatgaacc tcagaaaaaa acatatctgt atagccatat
     6721 cacttgttct taatccggca tccatatcac aggtcagtgc tcaggcagat acaaataagt
     6781 taactgttga agttgatgaa accagccttg gccatgatgt ttatggcgat gactggaaga
     6841 gcattgaaat aggtacaacg aaatttacag caaaagatga atcaaaggga attaatttcg
     6901 accataaatc accggtggct gaaggacgta actctatcgc gattggtacc agtgcagagg
     6961 ctggaggtga cattagcacg aaggaaggtt tgtacacagg cgagtctatt gcgataggag
     7021 cgaaaacgaa agcaactaaa gaacagtcaa ttgcaatagg tggcgatacc aaagctaccg
     7081 gatggggggc tattgttatt ggtggggatg atttaactcc tttgcatggt aagacttatg
     7141 gtcgggaaaa aatagagaat aactatctgg catcggaagc gaagggggat ggcagtattg
     7201 cgctgggcag cagaactttt gctaaaggcg aattgtcagt atcgcttggt tcggtgagtt
     7261 ctgctgaggg gatggcctcg aatgcaattg gagccacggc ggaagctaaa ggggatatgg
     7321 cgactgcggt tggttttaat gcgctggcta atgccggtaa tgctctggcc gttggtagtc
     7381 agtcctctgc aaataaaaac gatgctcttg ctgttggttc agaggcaaat gccgctcaga
     7441 atgaaacggt tgccctgggg catggagcaa ccgttactgt cgcaaagggg aacgtgtcca
     7501 tcggcgctga ctccagggat aagcaggcac aaaaagtaac agaggctaag gtcagaacta
     7561 aaagaggcgc ggaagtcaca tatagtggct ttgccggaaa taacgcagca tcagtagtat
     7621 ctgttggtga tgcgggtaaa gagcgtcaga ttgttaacgt gggggcgggt gctgtatctg
     7681 agaaaagtac agatgccatt aacggtagtc agttatatgc gatagcgaag acactgaaag
     7741 atgaccttga tgccattaat ccgacgtgga cgctaaagga tgataaggga ggttctgcga
     7801 ctattaatgg tgggaaggat aataagaatg gtaacacttt aactgttaaa ggtgatggag
     7861 atattgcagt tactgttaat gataaaggaa tgacgattga cggaacaacg ctgaaaaatg
     7921 aaataaaaaa tgatgccatc aaaattaaag gtggagatgg taagacgctg acgaagagtc
     7981 ttggtgacaa tgtcgaaatc actggcaaag gcccaataaa aacccaggtg gctggagata
     8041 aactggagat ttcagcagag acagcttcgt tgcctgtaaa tggtggtaac gggaagatta
     8101 cagtaaacaa tggtgatggc gataaacttg ttactgcgaa aaacattgct gatgccataa
     8161 accagtctgg ctggaaaatt aaagcggata aagataccgg tggtgttatc gagggaaatg
     8221 gtcagggaac agaaacactg ataaaacccg gtgatatggt tacgctgaaa gccggtaaaa
     8281 atctcagcgt gaaacaaagt aacggtgatt ttacattctc tgttattgat actccgtcat
     8341 tcagcagtgt tcagctgaat ggaaaaggaa ataacggaag tgtaacgctc tcatccgaag
     8401 gtaataagct gacattgtca ggcggtaatg gtgccggaca aaatggtgtt gttatcagtg
     8461 gtgtggcaag tggactggaa ggtaaggaac tttcgacgtt aacgaatact gataaaga
//
LOCUS       DAZGSP010000160         8461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZGSP010000160 DAZGSP010000000
VERSION     DAZGSP010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.161"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            84..974
                     /gene="alsR"
                     /locus_tag="ACHK74_004719"
     CDS             84..974
                     /gene="alsR"
                     /locus_tag="ACHK74_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose utilization transcriptional regulator
                     AlsR"
                     /protein_id="HFP0223413.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            1033..1968
                     /gene="alsB"
                     /locus_tag="ACHK74_004720"
     CDS             1033..1968
                     /gene="alsB"
                     /locus_tag="ACHK74_004720"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HFP0223414.1"
                     /translation="MNKYLKYFSSTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKILVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            2095..3627
                     /gene="alsA"
                     /locus_tag="ACHK74_004721"
     CDS             2095..3627
                     /gene="alsA"
                     /locus_tag="ACHK74_004721"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HFP0223415.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKPDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHETVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCCPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            3606..4586
                     /gene="alsC"
                     /locus_tag="ACHK74_004722"
     CDS             3606..4586
                     /gene="alsC"
                     /locus_tag="ACHK74_004722"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HFP0223416.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGV
                     DPFLAAMIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            4597..5292
                     /gene="alsE"
                     /locus_tag="ACHK74_004723"
     CDS             4597..5292
                     /gene="alsE"
                     /locus_tag="ACHK74_004723"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HFP0223417.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLATKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHDMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            5276..6205
                     /gene="alsK"
                     /locus_tag="ACHK74_004724"
     CDS             5276..6205
                     /gene="alsK"
                     /locus_tag="ACHK74_004724"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0019316 - D-allose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HFP0223418.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPS
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPHQVV
                     RFIAASSSDFNGAQGAAILAHQHFLPQSCAKAP"
     gene            6478..>8461
                     /gene="yjcS"
                     /locus_tag="ACHK74_004725"
     CDS             6478..>8461
                     /gene="yjcS"
                     /locus_tag="ACHK74_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl/aryl-sulfatase"
                     /protein_id="HFP0223419.1"
                     /translation="MNNSRLFHLSRIIIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         2208 a         2055 c         2143 g         2055 t
ORIGIN      
        1 tttttttgtg aagtagccag catcttttct gttcttgctg tggtgatata gtggcgtctt
       61 caattcaagg acaagagaac gtgatgagcc agtcagagtt tgattcagcg cttccgaacg
      121 gtatagggtt agcgccttac ctgcgaatga aacaggaagg aatgacagaa aatgaaagcc
      181 gcatcgtgga gtggttactc aaacccggta acctgagttg tgcacccgca attaaagatg
      241 tcgcagaagc tctggcggta tctgaagcga tgatagttaa ggtatcaaag ctgctggggt
      301 ttagcggctt tcgtaactta cgcagtgcgc tggaagatta tttttctcag tcagaacaag
      361 tattgccttc cgagttggct tttgatgaag cgccgcagga tgtggtgaat aaggtattta
      421 acatcacttt acgcaccatt atggaaggtc agtcgatcgt caacgttgat gagatccacc
      481 gtgccgcccg ctttttctat caggccagac agcgggattt atacggtgcc ggaggatcaa
      541 atgctatctg tgctgatgta cagcacaagt tcttgcgcat tggcgtacgc tgtcaggcct
      601 atccggacgc tcacatcatg atgatgtccg cttcgttgtt acaggaagga gatgttgtgc
      661 tggtagtgac ccattccggg cgaaccagtg atgtaaaagc ggccgtagaa ctggcaaaaa
      721 agaacggggc aaagattatt tgtataacgc atagctacca ttcaccaata gcgaaactgg
      781 ccgattatat tatttgctca ccagccccgg aaacgccgtt attaggtcgt aatgcctcgg
      841 caagaatatt acaactaact ttgctggacg ctttttttgt ctctgtcgcc cagctcaaca
      901 ttgaacaagc taatattaat atgcaaaaaa ccggcgcaat tgttgatttc ttctcaccag
      961 gcgcgctgaa ataagaaaaa ggtaattccg ccatcgcgga gttacctgtc cccttaccgt
     1021 gagaacaata ttatgaataa atatctgaaa tatttcagca gcacactcgt gggcttaatg
     1081 ttgtcaacca gcgcttttgc tgccgccgaa tatgctgtcg tattgaaaac cctctccaac
     1141 ccattttggg tagatatgaa aaaaggcatt gaagatgaag caaaaacact gggcgtcagc
     1201 gttgatattt ttgcctctcc ttcagaaggc gattttcaat ctcaattgca gttatttgaa
     1261 gatctcagta ataaaaatta caaaggtatc gccttcgctc cattatcctc agtgaatctg
     1321 gtcatgcctg tcgcccgcgc atggaaaaaa ggcatttatc tggttaatct cgatgaaaaa
     1381 atcgacatgg ataatctgaa aaaagctggc ggcaatgtgg aaggttttgt caccaccgat
     1441 aacgttgctg tcggggcgaa aggcgcgtcg ttcattattg acaaattggg tgctgaaggt
     1501 ggtgaagtcg caatcattga gggtaaagcc ggtaacgcct ccggtgaagc gcgtcgtaat
     1561 ggtgccaccg aagccttcaa aaaagcaagc cagatcaagc ttgtcgccag ccagcctgcc
     1621 gactgggacc gcattaaagc actggatgtc gccactaacg tgttgcaacg taatccgaat
     1681 attaaagcga tctattgcgc gaatgacacg atggcaatgg gtgttgctca ggcagtcgca
     1741 aacgccggaa aaacgggcaa aatattggtc gtcggtacag atggaattcc ggaagcccgc
     1801 aaaatggtgg aagccggaca aatgaccgcg acggttgccc agaacccggc agatattggc
     1861 gcaacgggtc tgaagctgat ggttgacgct gagaaatccg gcaaggttat tccgctggat
     1921 aaagcaccgg aatttaaact ggtcgattca atcctggtca ctcaataaaa ctgaatgatg
     1981 ccggatgatg atgctaatga agtgtcttat ccggccaaca aaataacctg aacgtagacc
     2041 tgataaacgt agccatcagg caataatacg atatttcgtg cagagaggtt atttatggcc
     2101 acgccatata tatcgatggc ggggattggc aagtcctttg gtccggttca cgcattaaag
     2161 tcggttaatt taacggttta tcctggtgaa atacatgcat tactaggaga aaatggcgcg
     2221 ggtaaatcca cgctaatgaa ggttttatcc ggaatacatg agccgaccaa aggcaccatt
     2281 actattaata acattaacta taacaagccg gatcataaat tagcggcaca actcggtatc
     2341 gggattattt atcaggaact cagcgttatt gatgaattaa ccgtactgga aaatttatat
     2401 attggtcgtc atctgacgaa aaaaatctgt ggcgtcaata ttatcgactg gcgagaaatg
     2461 cgtgtccgcg ccgccatgat gttattacgc gtgggcttga aagttgattt agatgagaaa
     2521 gtggcgaatt tatctatcag ccacaagcag atgctggaaa ttgccaaaac gctgatgctc
     2581 gatgccaaag tcatcatcat ggatgaaccc acctcctcac tcaccaataa agaggtggac
     2641 tatctgtttc tgatcatgaa tcagttgcgt aaagagggta cggccatcgt ctatatctcg
     2701 cataagttgg cggaaattcg ccgtatttgc gaccgctata cggtgatgaa agacggcagc
     2761 agcgtttgca gcggcatggt gagcgatgtg tcaaatgacg atatcgtccg tctgatggta
     2821 ggccgcgaat tgcaaaaccg ttttaacgcg atgaaggaga atgtcagcaa ccttgcgcac
     2881 gaaacggttt ttgaggtgcg caacgtcacc agccgtgaca gaaaaaaggt ccgggatatc
     2941 tcatttagcg tctgccgggg agaaatatta ggctttgccg ggctggtcgg ttccggacgt
     3001 actgaactga tgaattgcct gtttggcgtt gataaacgcg ctggcggaga aatccgtctt
     3061 aatggcaaag atatctctcc acgttccccc ctggatgccg tgaaaaaagg gatggcttac
     3121 atcactgaaa gccgccggga taacggtttt ttccccaact tttccatcgc tcagaacatg
     3181 gcgatcagcc gcagtctgaa agacggcggc tataaaggcg cgatgggctt gtttcatgaa
     3241 gttgacgagc aacgtaccgc tgaaaatcaa cgcgaactgc tggcgctgaa atgtcattcg
     3301 gtaaaccaga atatcaccga actctccggg ggaaatcagc agaaagtcct gatctccaaa
     3361 tggctgtgct gttgcccgga agtgattatt ttcgatgaac ctacccgcgg catcgacgtt
     3421 ggcgcgaaag ccgaaattta caaagtgatg cgccaactgg cggacgacgg aaaagtcatc
     3481 ctgatggtgt catctgaact acctgaaatt atcaccgtct gcgaccgcat cgccgtgttc
     3541 tgcgaaggac gactgacgca aatcctgacg aatcgcgatg acatgagcga agaggagatt
     3601 atggcatggg ctttaccaca agagtaaaaa gcgaagcgag cgagaagaaa ccgttcaact
     3661 ttgcgctgtt ctgggataaa tacggcacct tttttatcct ggcgatcatc gtcgccattt
     3721 ttggttcgct gtcgccagaa tattttctga ccaccaataa tattacccag atttttgttc
     3781 aaagctccgt gacggtattg atcggcatgg gcgagttttt cgctatcctg gtcgctggta
     3841 tcgacctctc ggttggcgcg attctggcgc tttccggtat ggtgaccgcc aaactgatgt
     3901 tggcaggtgt tgacccgttt ctcgcagcga tgattggcgg tgtactggtt ggcggcgcac
     3961 tgggggcgat caacggctgc ctggtcaact ggacggggct acacccgttc atcatcaccc
     4021 ttggcaccaa cgcgattttc cgtgggatca cgctggtgat ctccgatgcc aactcggtat
     4081 acggcttctc ctttgacttc gtgaacttct ttgccgccag cgtaattggg atacctgtcc
     4141 ccgttatctt ctcgctaatt gtcgcgctca tcctttggtt tctgacaacg cgtatgcggc
     4201 tcgggcgcaa catctacgca ctgggcggca acaaaaattc ggcgttctat tccgggattg
     4261 acgtgaaatt ccacatcctg gtggtgttta tcatctccgg tgtttgtgca ggtctggcag
     4321 gcgtcgtctc aactgcacga ctcggtgccg cagaaccgct tgccggtatg ggttttgaaa
     4381 cctatgccat tgccagcgcc atcattggcg gcaccagttt cttcggcggc aaggggcgca
     4441 ttttctctgt ggtgattggc gggttgatca tcggcaccat caacaacggt ctgaatattt
     4501 tgcaggtaca aacctattac caactggtgg tgatgggcgg attaattatc gcggctgtcg
     4561 cccttgaccg tcttatcagt aagtaaggaa ttgaacatga aaatctcccc ctcgttaatg
     4621 tgtatggatc tgctgaaatt taaagaacag atcgaattta tcgacagcca tgccgattac
     4681 ttccacatcg atatcatgga cggtcacttt gtccccaatc tgacactctc accgttcttc
     4741 gtaagtcagg ttaaaaaact ggcaactaaa ccgctcgact gtcatctgat ggtgacgcgg
     4801 ccgcaggatt acattgctca actggcacgt gcgggagcag attttatcac tctgcatccg
     4861 gaaaccatca acggccaggc gttccgcctg attgatgaaa tccgccgtca tgacatgaaa
     4921 gtggggctga tccttaatcc ggagacgcca gttgaggcca tgaaatacta tatccataag
     4981 gccgataaaa ttacggtcat gactgtcgat cccggctttg ccggacaacc gttcattcct
     5041 gaaatgctgg ataaacttgc cgaactgaag gcatggcgtg aacgagaagg tctggagtac
     5101 gaaattgagg tggacggttc ctgcaaccag gcaacttacg aaaaactaat ggcggcagga
     5161 gcggatgtct ttatcgtcgg cacttccggc ctgtttaatc atgcggaaaa tatcgacgaa
     5221 gcatggagaa ttatgaccgc gcagattctg gctgcaaaaa gcgaggtaca gcctcatgca
     5281 aaaacagcat aacgtcgtag cgggcgtgga tatgggggca acgcatatcc gcttttgtct
     5341 gcggacagca gaaggtgaaa cgctacactg cgaaaaaaag cggaccgcag aagtcattgc
     5401 tcccagcctg gtgtcgggta tcggcgaaat gattgacgag caactcaggc gctttaacgc
     5461 tcgctgtcat ggtctggtga tgggatttcc ggcgctggtc agtaaagata aacgcaccat
     5521 aatttctacg cctaacctgc cgttaacagc ggcggattta tatgatctcg ccgataagct
     5581 cgaaaatacg ctgaattgtc cggttgagtt ttcccgcgac gttaacctgc aactctcctg
     5641 ggacgtagta gaaaaccgcc ttacgcaaca actggttctg gcggcctatc tcggtacggg
     5701 gatggggttc gcagtgtgga tgaacggtgc gccgtggacg ggtgcacacg gtgtggcagg
     5761 cgaactgggt catatccccc tgggagatat gacccaacac tgcgcgtgtg gcaatcctgg
     5821 gtgcctggaa accaattgct ctggaatggc gctaagacgc tggtacgaac aacagccccg
     5881 aaattaccca ttgagcgacc ttttcgtcca tgcggaaaac gcccctttcg tccagagtct
     5941 gcttgaaaac gcggcacggg ccattgccac cagcattaat ctgttcgatc ccgatgcggt
     6001 gattctgggc ggtggcgtga tggatatgcc cgccttccca cgcgagactc tcattgccat
     6061 gacccaaaag tacctgcgcc gtccactgcc gcatcaggtc gtgcgcttta ttgccgcctc
     6121 atcttctgac tttaatggcg ctcagggtgc agcaatattg gcgcatcaac attttttgcc
     6181 acagtcctgt gctaaagccc catgagtaaa gcgttggcac agtagcgcaa tgtcccgctg
     6241 tgccaacgac aggtacaata tatataataa acacataaaa cggctcatat tattttctgc
     6301 aatgatagtt ttactgtaac ttttcctctt cagcacaaat gcctaataaa atggatcgtt
     6361 cagctcacac ttccacttat taaagtcgca aattcccaat tatgataacc ccagctttaa
     6421 atgacgtatt ttgcatggat tacatattta cgacataaat gataaatgga gatttatatg
     6481 aataactctc ggttattcca tttgagcaga attattattg cgttaactgc cgccagcggc
     6541 atgatggtaa ataccgctaa cgcgaaagag gaagcgaaag ccgccactca atatacccaa
     6601 caggttaatc agaattacgc caaatcatta ccgtttagcg atcgtcagga ttttgacgac
     6661 gctcagcgtg gatttatcgc tccgctgctg gatgaaggta ttctgcgcga tgctaatggc
     6721 aaaccatatt atcgcgggga agattataaa tttgatatca atgcgcccgc gcctgaaacc
     6781 gttaacccta gcctgtggcg tcagtcgcag ttaaacggta tttccggcct gtttaaagtc
     6841 accgacagaa tgtaccaggt tcgcggtcag gatatctcca acatcacctt tatcgaaggt
     6901 gataccggga ttattgtcat cgacccgctg gtgacgccac cgagcgcaaa agccgccctt
     6961 gacctttact tccaaaatcg cccgcaaaaa ccgattgttg cggttattta tacccacagc
     7021 cataccgacc actatggcgg cgtgaaaggc attatctctg aagccgatgt gaaatccggt
     7081 aaagtgcagg ttatcgcccc tgctggcttt atggacgaag ccatcagcga aaacgtactg
     7141 gcgggtaata ttatgagccg ccgtgcactt tactcctacg gcctgctgct ggcgcataac
     7201 cctcagggta acatcggcaa tggtcttggc gtaacgctgg catcgggcta cccgagcatc
     7261 atcgcaccga acaaaaccat caccaaaacc ggtgagaaga tgattatcga cggcctggag
     7321 tttgacttcc tgatgacccc aggtagcgaa gcaccagccg aaatgcactt ctatattccg
     7381 gccctgaaag cgctgtgtac cgccgagaac gccacgcata ccctgcacaa cttctacact
     7441 ctgcgcggcg cgaaaacccg cgacaccagc aagtggaccg agtatctgaa cgaaacgctg
     7501 gatatgtggg gtaacgacgc ggaagtcctg tttatgccgc acacctggcc ggtctggggc
     7561 aataagcata tcaatgatta tattggtaaa tatcgcgata ctatcaagta cattcacgac
     7621 cagaccctgc acctggcgaa ccagggctac accatgaatg aaatcggcga catgattaaa
     7681 ctgccgcctg cacttgccaa taactgggcc agccgtggct attacggttc tgtcagccac
     7741 aacgcccgcg cggtgtataa cttctatctt ggctattacg acggtaaccc ggctaacctg
     7801 catccgtatg gtcaggtgga gatgggtaaa cgttacgtgc aggcgctggg cggttctgcc
     7861 cgtgtcatca acctggcgca agaagcgaac aagcaaggtg attaccgctg gtcggcagaa
     7921 ctgctgaaac aggtgattgc cgccaacccg ggtgaccagg tcgcgaagaa tctgcaagcg
     7981 aataactttg aacagctggg ctatcaggcc gagtccgcca cctggcgcgg tttctacctg
     8041 accggcgcga aagagctgcg cgaaggggtg cataagttca gccacggcac caccggttcc
     8101 ccggacacca ttcgcgggat gtcggtcgaa atgctgttcg actttatgtc cgttcgcctc
     8161 gatagcgcga aagccgcggg taaaaatatc agcctgaact tcaatatgag caatggcgat
     8221 aacctcaacc tgacgctgaa cgatagcgtg cttaactacc gtaaaacact gcaatcccaa
     8281 gctgacgcct ctttctacat cagccgtgaa gatctgcacg ccgtgctgac cgggcaagcc
     8341 aaaatggcgg atctggtaaa agcgaagaaa gccaaaatta ttggcaatgg cgcgaaactg
     8401 gaagaaatta tcgcctgtct ggataatttc gatttgtggg tgaatatcgt aaccccaaat
     8461 t
//
LOCUS       DAZGSP010000161         8443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZGSP010000161 DAZGSP010000000
VERSION     DAZGSP010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.162"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(104..180)
                     /locus_tag="ACHK74_004726"
     tRNA            complement(104..180)
                     /locus_tag="ACHK74_004726"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Asp,seq:gtc)
     gene            complement(313..1044)
                     /gene="dnaQ"
                     /locus_tag="ACHK74_004727"
     CDS             complement(313..1044)
                     /gene="dnaQ"
                     /locus_tag="ACHK74_004727"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFP0223420.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1109..1576
                     /gene="rnhA"
                     /locus_tag="ACHK74_004728"
     CDS             1109..1576
                     /gene="rnhA"
                     /locus_tag="ACHK74_004728"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFP0223421.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHSWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1573..2295)
                     /locus_tag="ACHK74_004729"
     CDS             complement(1573..2295)
                     /locus_tag="ACHK74_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP0223422.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALEHQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQYRKPQA"
     gene            2329..3084
                     /gene="gloB"
                     /locus_tag="ACHK74_004730"
     CDS             2329..3084
                     /gene="gloB"
                     /locus_tag="ACHK74_004730"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFP0223423.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3156..4514
                     /gene="mltD"
                     /locus_tag="ACHK74_004731"
     CDS             3156..4514
                     /gene="mltD"
                     /locus_tag="ACHK74_004731"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFP0223424.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4562..5332)
                     /locus_tag="ACHK74_004732"
     CDS             complement(4562..5332)
                     /locus_tag="ACHK74_004732"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP0223425.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(5410..6210)
                     /locus_tag="ACHK74_004733"
     CDS             complement(5410..6210)
                     /locus_tag="ACHK74_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP0223426.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6451..7365
                     /gene="yafC"
                     /locus_tag="ACHK74_004734"
     CDS             6451..7365
                     /gene="yafC"
                     /locus_tag="ACHK74_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFP0223427.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMASAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7362..8165)
                     /gene="dkgB"
                     /locus_tag="ACHK74_004735"
     CDS             complement(7362..8165)
                     /gene="dkgB"
                     /locus_tag="ACHK74_004735"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFP0223428.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKTIAAVGAENIATNQIELSPYLQ
                     NRKVVVWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8328..8404)
                     /locus_tag="ACHK74_004736"
     tRNA            complement(8328..8404)
                     /locus_tag="ACHK74_004736"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8368..8370),aa:Asp,seq:gtc)
BASE COUNT         2251 a         2032 c         2048 g         2112 t
ORIGIN      
        1 ccagaataaa atcattgaac agagaaatgg tggaattcaa atacaaaaaa accgcaaaat
       61 taaaaatctt gcggctctct gaactcattt tcatgagtga atttggcgga acggacggga
      121 ctcgaacccg cgaccccctg cgtgacaggc aggtattcta accgactgaa ctaccgctcc
      181 gcgttgtgtt ccgttgggaa cgaggcgaat attacggatt gcctcaccct tcgtcaacgg
      241 tttttctcat cttttgaatc gtttgctgca aaaatcgccc aagccgctat ttttagcgcc
      301 ttttacaggt atttatgccc gccagaggca gcttccgccc ttcttctcca ccagatcaag
      361 acgggcttca tgagctgcaa gctcttcatc tgtcgcaaaa acaacgcgta acttacttgc
      421 ctgacgtaca atgcgctgaa ttgttgcttc accttgttgc tgctgtgtct ctccttccat
      481 cgcaaaagcc atcgacgttt gaccaccggt catcgccaga taaacttccg caaggatctg
      541 tgcatcgagt aatgccccgt gtagcgttcg tttactgtta tctatttcgt agcgggcaca
      601 taacgcatcg aggctgttgc gcttaccagg aaacattttc ctcgccaccg caaggctatc
      661 ggtgacctta cagaaggtat tggtcttcgg aatatcgcgc ttaagcaacg aaaactcgta
      721 gtccataaag ccgatatcga acgctgcgtt atggatcacc aactccgcgc cgcggatata
      781 gtccatgaac tcatcggcta cttcggcaaa cgtgggctta tcgagcaaaa attcatcggc
      841 aataccatgt acgccaaagg cttccggatc caccagccga tcgggtttga gataaacatg
      901 gaagttattg cccgtcaggc gacggttcac cacttcaacg gcaccaatct caatgatctt
      961 gtggccttca tagtgcgcac caatctggtt cataccggtg gtttcggtat cgagaacgat
     1021 ctggcgtgta attgcagtgc tcatagcggt catttatgtc agacttatcg ttttacagtt
     1081 cgattcaatt acaggaagtc taccagagat gcttaaacag gtagaaattt tcaccgatgg
     1141 ttcgtgtctg ggcaatccag gacctggggg ttacggcgct attttacgct atcgcggacg
     1201 cgagaaaacc tttagcgcag gctacacccg caccaccaac aaccgtatgg agttgatggc
     1261 cgctattgtc gcgctggagg cgttaaaaga acattgcgaa gtcattttga gtaccgacag
     1321 ccagtatgtc cgccagggga tcactcagtg gatccatagc tggaaaaaac gtggctggaa
     1381 aaccgcagac aaaaaaccag taaaaaatgt cgatctctgg caacgtcttg atgctgcatt
     1441 ggggcagcat caaatcaaat gggaatgggt taaaggccat gccggacacc cggaaaacga
     1501 acgctgtgat gaactggccc gtgctgcggc aatgaatccc acactggaag atacaggcta
     1561 tcaagtcgaa gtttaagcct gtggtttacg atattgccgg gtggctccaa ccgcctggcg
     1621 aattcgtggc ttgtttttac tctgtttcat cggatttagc gttaaaggaa tagtccgttt
     1681 ccgggcaaca ataagttgta agcaaccaag cgcaggaata tgtgcattca atagttttcc
     1741 accgtgtttg ttccacggga gaacgtggaa acggctggcg tgcagcactt caaaattcaa
     1801 caaagagagc cagtccagtt gccgcatcag agtaaacatc cggctgttat agggcgaggt
     1861 tttgcgcaat accggcacaa gtttgcgtaa tcccattaaa ctgatgggat tgaagccact
     1921 aatcaccagc cagccatcat caatcaatac ccgatcggct tcacgcaata aacgatgcgg
     1981 atcggtgcac cacggcaatg tatgtgccag tagacaaaca tcaacggatt tatcggcaaa
     2041 aggaagatga agtgggtccg cctgtacctg gacgggcatt ccttgcgcag aaacattcac
     2101 ttgatgagaa accgcgcacg cttcgcaatt gatttctgcg cttaaattgc caatcttaag
     2161 cagatgaaaa ccatacattt tagtgaacca cgggttgagc tggtgctcca gcgccttgcg
     2221 ataaagctct ccccagggca aatcgcccca gcaatcagga gccacgacag tttgagggac
     2281 tcttgccggt ttcatcacaa ccttccgttt cacactaaga ggtaatctat gaatcttaac
     2341 agtattcccg cctttgatga caattacatc tgggttttga atgatgaagc aggtcgctgc
     2401 ctgattgtcg atcccggaga cgcagagcca gtattaaacg ccattaccgc caataactgg
     2461 caaccggagg ccatatttct cacccaccat catcacgatc acgttggcgg cgtaaaagaa
     2521 ctggtggaaa agtttccgca aattgtggtg tatggtccac aagagacaca agataaggga
     2581 acaacacagg tagtcaaaga tggcgaaact gccttcgttt tggggcatga atttagtgta
     2641 attgccacac cgggtcacac tttaggacat atctgttact tcagtaaacc ttatctattt
     2701 tgcggcgaca cgctgttttc tggtgggtgt ggtaggttgt ttgaagggac agcatcacaa
     2761 atgtatcaat cacttaataa gttaagtgcg ttacctgacg atacattggt atgttgtgct
     2821 catgaatata cgttatcaaa tatgaagttt gctttgagta ttcttccgca cgatttgtcc
     2881 ataaatgatt attatcgtaa agttaaggag ttacgggcaa aaaatcaaat aacactaccc
     2941 gtaattctga aaaatgagcg gcaaattaat gtttttttaa gaacagaaga tattgattta
     3001 attaatgtaa ttaatgaaga aacattattg caacaacctg aagaacgttt tgcatggtta
     3061 aggtcaaaga aagataggtt ctgataaaac tttcttgtca tcggctccgt tcgccgttat
     3121 gatcggtcgt cttttaagca actattgaca cacacatgaa ggcaaaagcg atattactcg
     3181 cctctgtcct gctcgtgggt tgccagagta ccggcaacgt tcaacagcac gcacagagtc
     3241 tttctgcagc tggtcaaggg gaagcagcaa agtttacaag tcaggcacga tggatggacg
     3301 atgggacgtc tatcgcgcca gatggtgact tgtgggcttt cattggcgac gagctaaaga
     3361 tgggaattcc ggaaaatgac cggattcgcg aacagaaaca gaaatattta cgcaataaga
     3421 gctatctcca cgatgtaact ttacgggcag agccgtatat gtactggata gccgggcaag
     3481 ttaaaaaacg taacatgcct atggaactgg tactactacc catagtggag agcgcttttg
     3541 atcctcacgc aacgtctggc gccaatgccg caggcatctg gcagatcatt ccgagcacgg
     3601 ggcgcaatta tggtttgaaa cagacccgca attatgacgc gcgtcgcgat gttgttgctt
     3661 caacaactgc cgcgctgaac atgatgcagc gtctgaacaa gatgtttgac ggcgactggc
     3721 ttctgaccgt agcggcttat aacagcggcg aaggtcgggt catgaaggca attaaaacga
     3781 acaaagcgcg tgggaaatcc acggacttct ggtcgttacc gttgccgcag gaaacgaagc
     3841 agtacgtgcc taaaatgctg gcattgagtg atattctcaa aaacagcaag cgttatggcg
     3901 tacgtctgcc aacgaccgat gaaagccgtg ctctggcgcg tgtgcacctg agcagcccgg
     3961 ttgaaatggc gaaggttgca gatatggcgg ggatttccgt cagcaagctg aagacattca
     4021 acgctggcgt gaaaggctcc acgctgggcg caagtggtcc gcaatacgtg atggtgccaa
     4081 agaagcatgc agatcaactg cgtgaatctc tcgcttcagg cgaaattgct gctgtacagt
     4141 cgacgctggt tgccgacaat acgccgctta acagccgtgt ttacaccgta cgctctggcg
     4201 acacgctttc aagtatcgct tcacgtctcg gcgtaagcac caaagatttg cagcagtgga
     4261 acaaactgcg cggatctaag ctgaagccag gccaaagttt gacgattggt gcaggcagta
     4321 gcgcacagcg actggcaaac aacagcgata gcattacgta tcgtgtgcgc aaaggcgatt
     4381 cgctttcaag cattgctaaa cgccacggcg tgaacatcaa agatgtgatg cgctggaaca
     4441 gcgacactgc gaatctgcaa ccaggcgaca agctgacgtt gtttgtgaaa aacaacaaca
     4501 tgccagattc ctgacaaacc agataataaa aaggcaccga ttcccccggt gcctttttta
     4561 tttatgctgc tttatgtgct tctaccatga tgatatcact ggtgaaagag ccatcattct
     4621 gcaaggcaaa atacgttctc acctctgtcg atgcgctctg ttggtaaatg cgaatagcgt
     4681 ctactaacgc ttctggcgta cgcattctcg cgacccatga agaaaattcc agcggtaact
     4741 tatctgtaat taaattatca actatcagat tggcttcatt gattaacctc aaccactcac
     4801 cgctggcgta gtttcgtacg tgagaggtat cgcgtaatgc ttctaccgtc tgtaaccaga
     4861 tgtcgcgcac tggatgaccc ggagacatta cgtccatcac aatcatccta ccgccaggtt
     4921 tcaatatcct attcacttct cgcagtgctg caccaacatc atgccagtga tgggcagaat
     4981 aacggctgat aacaatatca aatgcgttat cggcaaatgg cagactttcg gcataaccct
     5041 ggcgggtggt gatatttttc agttgccggg cttcggcagc ttgtgccacg acatccagca
     5101 tttgggcaga taagtcatac gccaccaccg cgctcacgtt ctgcgcagcg acaaagctgg
     5161 catgccctgc tccgcagccc atatcaagta cacttgcatc aggataatca gccagacgca
     5221 ccgccaggcg ctgtaaatct cgcccggatg catgtacggt actggttaaa tactcactgg
     5281 cctgcgagct aaactgcttt tctacgtggt catggtggga ttgtgttgtc attgcgctgt
     5341 ccttttgttg tttgagtata aaaggacagc gccaaacgtt ggtctgccct gtggcagacc
     5401 tgacatacct tatttatcag gcttgccggg actgaattca acgagtagcg gattgtgatc
     5461 ggaagcgcgc gtaaccaata cagaagcttc gctgacgttc agaccacggt agaaaacaaa
     5521 atcgagcggg cggccaaacg cccgacggcg ctgatcatcg gtaaaacgca cctggcgcag
     5581 cgacatttcc cgcgcaaagc gatataacgc gttcatcctt ctacggctcc aggcattgaa
     5641 atctcctgcc ataatgaccg ggccgctgtg gtgagctatc tgatcaccaa taggaagtaa
     5701 ctgcttacta tagacatcca cgcccagact gaagttgacg gcgtgtatat taaccaccat
     5761 caacaggcgg gtgtcaggta atggatagac cgtcaccagt gccgacttcg ccagacgcaa
     5821 aatgggttct cgttcacgta acgggcagca atacactgga tgcgccgccg aaagggtcat
     5881 tacgccagaa ggatgttgtg gcagtacgaa agcgggtacc tgatcggcgg caagatagtt
     5941 agcggtcgca aactgtacta actctggcgt tgtctgcgct tcctgcaata acaccagatg
     6001 tgcatctttg ccgtagttct ttaataccga caaccattca gcgcgttgct gtttgtatat
     6061 gttccacacc aggatccgaa tacgctcttc ggtacttaac ggttctccgg gtggtaatgc
     6121 ctggccgatg ctcgcaaaag accccggcgg taagatcctt tccgcaggtt gtccggcaac
     6181 atagcgcatg gcataggtgt tttttcgcac ttttgacttt gaaacctcta ttacgtcaac
     6241 ccttccgcac tcgggagggt tctactgtta tagggatttt agctcactct ttcaacgagc
     6301 aattgtatat tgttatgtaa gcaagtgctt acaccggtca gaattttgct cattatggag
     6361 aacattaaat cgcatgacat atcacgcatc ctgatggttt aatgacaaat aaattcaaaa
     6421 tatctcttct attttgcaac aggagcaaaa atgaaagcca cgtcggaaga actcgccatt
     6481 tttgtttcgg tcgtcgaaag cggcagcttt agccgggcag cggaacaatt agggcaagca
     6541 aactcagcgg taagccgggc ggtgaaaaag ctggagatga aacttggcgt tagcctgctt
     6601 aatcggacca cgcgacaact tagcctgacg gaagaaggcg agcgttattt tcgtcgcgta
     6661 cagtcaattt tgcaggagat ggcatcggca gaatcagaaa ttatggagac gcgtaataca
     6721 ccgcgtggac tgttacggat cgatgccgca actccagtgg tgctgcactt tctgatgccg
     6781 ttaattaagc ctttccgtga acgctatccg gaagtcactt tgtcgctagt ctcctccgaa
     6841 acgattatta atttgatcga aagaaaagtg gatgtcgcga tacgcgctgg tacgttaacg
     6901 gattccagct tacgtgccag gccgttattt aacagttatc gaaaaattat cgcctccccc
     6961 gattatattt cccgctacgg gaagccagaa acgatcgacg atttaaagca acatatttgc
     7021 ctgggattca ctgaacccgc ttccctcaat acctggccga tagcccgtag cgatggacaa
     7081 ttacatgagg tgaagtacgg tttgtcatcc aatagtgggg aaacactgaa acagctttgc
     7141 ctgagtggca acgggattgc gtgtttgtcc gactacatga tcgacaaaga aatcgctcgc
     7201 ggagaattgg tggagttaat ggcagataaa gtgttgccag tggaaatgcc attcagtgca
     7261 gtctattaca gcgaccgtgc ggtaagtacg cgcatccggg cttttatcga tttccttagc
     7321 gagcatgtaa aaacagctcc cggaggagct gtcagagagg cttaatccca ttcaggagcc
     7381 agaccttccg ggctaaccag gcggtcgttg caatccagtg cggcgatcgc ttttttatct
     7441 tcggcatcaa gctgtaaatt ttgtgcctta agattacttt ccaggttttc acgtttagta
     7501 gaagaaggaa ttactgagta accttccccc atagcccacg ccagaatcac ttgtgccgga
     7561 gtcgcattgt gtttagctgc gatacgagca ataacctcat ctttcagggc cttaccatac
     7621 gccagcgtca tataggaagt aatatggatg ccgtgctgtt tagcccagac aaccacttta
     7681 cggttttgca gataaggaga gagttcaatc tggttagtag cgatgttttc agcgccaaca
     7741 gcagcaatcg ttttttccat caacgggatc gtgaagttgg aaataccgat ctcacgtgtc
     7801 agaccttgtt ttttggcttc cagcagcgcc tgcataaact cttcaacaga gacttcatcg
     7861 tttggtgacg gccagtggat tagcgtcaga tcaacataat cggtacgcaa tttttgcagg
     7921 ctctctttca gacttgggat caatttgtct ttgctgagat tttcaatcca gattttagtg
     7981 gtgatgtaga gttcatgacg tggcacgcca ctttctgcaa tcgcctgacc tactgcggct
     8041 tcgttatcat agatttgtgc ggtatcaatt gcgcgataac caagttcaag cgccgttttc
     8101 acagatgaaa taacaacgtc gtctttcaga cggaaagtac ctaaaccaaa tgcagggata
     8161 gccataatat gcctctttta atgctatttt cgttaactga ggtgattatg ggcttcagac
     8221 tgttgaggaa aaagagcgta aaatgcagag gatttttgcg attctggcaa taatagatat
     8281 acagacgaaa aaaaacctcg agcgttaact cgaggcttct taataagtgg cggaacggac
     8341 gggactcgaa cccgcgaccc cctgcgtgac aggcaggtat tctaaccgac tgaactaccg
     8401 ctccaccgaa ttcttttaca accaccggtt ttatgaccgg ctt
//
LOCUS       DAZGSP010000162         8335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZGSP010000162 DAZGSP010000000
VERSION     DAZGSP010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.163"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(77..283)
                     /locus_tag="ACHK74_004737"
     CDS             complement(77..283)
                     /locus_tag="ACHK74_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFP0223429.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            596..685
                     /gene="kdpF"
                     /locus_tag="ACHK74_004738"
     CDS             596..685
                     /gene="kdpF"
                     /locus_tag="ACHK74_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFP0223430.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            685..2358
                     /gene="kdpA"
                     /locus_tag="ACHK74_004739"
     CDS             685..2358
                     /gene="kdpA"
                     /locus_tag="ACHK74_004739"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFP0223431.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2381..4429
                     /gene="kdpB"
                     /locus_tag="ACHK74_004740"
     CDS             2381..4429
                     /gene="kdpB"
                     /locus_tag="ACHK74_004740"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFP0223432.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRQMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4438..5010
                     /gene="kdpC"
                     /locus_tag="ACHK74_004741"
     CDS             4438..5010
                     /gene="kdpC"
                     /locus_tag="ACHK74_004741"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFP0223433.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVKLNLALDKLDE"
     gene            5003..7687
                     /gene="kdpD"
                     /locus_tag="ACHK74_004742"
     CDS             5003..7687
                     /gene="kdpD"
                     /locus_tag="ACHK74_004742"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFP0223434.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7684..>8335
                     /gene="kdpE"
                     /locus_tag="ACHK74_004743"
     CDS             7684..>8335
                     /gene="kdpE"
                     /locus_tag="ACHK74_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFP0223435.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITET"
BASE COUNT         1724 a         2181 c         2439 g         1991 t
ORIGIN      
        1 cctctgaaca tcttcctgat acaaaaaacc gccagcgttt ccgctggcgg ttttcaatgt
       61 attcaaaact cagcaatcaa taaaaatcac cagttgcctt ttcggcctga tccatccaca
      121 ccggtttatc gctggttttc gaccagacgc gatgcaggta gctgtaaaag cgggcgcggt
      181 ctttccagaa aagcatgaaa ggcagcgcca gaacgcccgc tgcaaccgca taagtacggc
      241 gtaaaaagat aagccatgca ggatattctc tgtagagttc catacttttc tccccctttt
      301 gtatctaccc ggtgaatggc accggaaaaa tgaatttgtt tatctgatga aaatagtacc
      361 gccttttgtg taattttact actcatccga ccacttattt ttgcttattg atggtttatt
      421 tacattcatc ctgtaattaa gttacacaaa agttaaatta atactaaaca ttagttaaat
      481 catggctttt gccattttta tacttttttt acaccccgcc cgcagatttt tgcgaaatct
      541 ttgcagccag aattctaccc ttccggtatc acttttaggc cactggaggt gcactgtgag
      601 tgcaggcgtg ataaccggcg tattgctggt gtttttatta ctgggttatc tggtttatgc
      661 cctgatcaat gcggaggcgt tctgatggct gcgcaagggt tcttactgat cgccacgttt
      721 ttactggtgt taatggtgct ggcgcgtcct ttaggcagcg ggctggcgcg gctgattaat
      781 gacattcctc ttcccggtac aacgggcgtt gagcgcgtac tttttcgcgc acttggcgtc
      841 tctgaccgtg agatgaactg gaagcaatat ctttgtgcca ttctcggcct gaacatgctg
      901 gggctggcgg tgctgttttt tatgttgctc ggtcagcact atctgccgct taatccacag
      961 cagttgccgg ggctgtcgtg ggatctggcg ctgaataccg ccgtcagctt tgtcacgaat
     1021 accaactggc aatcttacag cggtgaaacc acgttgagct atttcagcca gatggctggc
     1081 ttaacggtgc aaaactttct ttctgccgcc agcgggattg cggtgatttt tgccctcatc
     1141 cgtgcgttta cccgccagag catgagcaca ctcgggaatg cctgggtcga tctgctacgc
     1201 atcacgttat gggtgctcgt ccctgtggcg ttgttgatcg cactgttttt tattcaacaa
     1261 ggtgcgctgc aaaactttca gccttatcag gctgtgaata ccgttgaagg agcgcaacag
     1321 ctgttaccca tggggcctgt agcttctcag gaagcgatca agatgctcgg tactaacggc
     1381 ggtggcttct ttaatgccaa ctcgtcgcat ccgtttgaaa acccaaccgc actgaccaac
     1441 ttcgtgcaga tgctggcgat cttcttgatc ccaacggcgc tgtgctttgc ctttggtgaa
     1501 gtgacgggcg atcgccgcca ggggcgcatg ttgctgtggg cgatgtcagt gatttttgtc
     1561 atctgcgtag gcgtggtgat gtgggcagaa gttcagggta atcctcatct gctggcgctc
     1621 ggcgcagaca gcagcatcaa tatggaaggt aaagagagcc gtttcggcgt gctggtcagt
     1681 agcctgtttg cggtcgtgac tacggcggct tcctgtggcg cggtgattgc gatgcatgat
     1741 tcgtttaccg ctctcggtgg catggtgccg atgtggctga tgcaaattgg tgaagtggtg
     1801 ttcggcggtg tcggttctgg tctttacggc atgatgctgt ttgtcctgct ggcggtgttt
     1861 attgccgggc tgatgattgg tcgtacaccg gaatatctgg gtaaaaaaat cgacgtacgc
     1921 gagatgaaac tgactgcact ggcgattctg gttaccccga cgctggtgct gatgggcgcg
     1981 gcgctggcaa tgatgaccga cgccggacgt agcgccatgc tcaaccctgg cccgcatggt
     2041 tttagcgaag tgctgtacgc cgtgtcatcc gccgctaaca acaacggcag cgcctttgcc
     2101 ggattaagcg ccaactcccc gttctggaac tgtttactgg cgttctgcat gtttgtcggt
     2161 cgcttcgggg tgattatccc ggtgatggca attgccggtt cgctggtgag taaaaagagc
     2221 caacccgcca gctccggcac gctgccaacg cacggcccgc tgtttgttgg cctgttaatc
     2281 ggcaccgtgt tgctggttgg cgcactgacc tttatccctg ccctggcgct tggtccggtg
     2341 gcggaatatc tctcctgatg atattgagtg agcactgaat atgagtcgta aacaactggc
     2401 gctattcgaa ccaacacttg tcgttcaggc gctgaaagaa gcggtgaaaa aattaaaccc
     2461 gcaggcgcaa tggcgcaatc cggtgatgtt tatcgtctgg atcggcagtc tgctgaccac
     2521 ctgtattagc atcgcgatgg caagcggtgc gatgcctggc aatgcgctgt ttagcgcggc
     2581 aattagcggc tggctgtggg tcaccgtact gttcgctaat ttcgccgagg cgctggcaga
     2641 aggccgcagt aaagcgcagg ccaacagtct taaaggggtg aaaaaaactg cctttgcccg
     2701 caagctgcgt gagccgaaat atggcgctgc ggcggacaaa gttcctgccg atcaactgcg
     2761 taaaggtgac atcgtgctgg tagaagctgg cgatattatc ccctgcgatg gtgaagtcat
     2821 tgaaggcggc gcatcggtcg atgaaagcgc catcaccggg gaatcggcac cggtgatccg
     2881 tgaatccggc ggcgattttg cctccgtcac cggcggcacg cgtattcttt ctgactggct
     2941 ggtgattgag tgtagcgtta accccggtga gacatttctg gatcggatga tcgcgatggt
     3001 ggaaggcgca cagcgacgca aaacaccgaa cgagattgcc ctgactatcc tgctgattgc
     3061 tctgaccatc gtctttttac tggcaaccgc cacgctgtgg ccgttttccg cgtggggcgg
     3121 taatgccgtg agtgtaacgg tactggtggc gctgctggtc tgtctgatcc caaccactat
     3181 tggcggcctg ttgtcagcga tcggcgtcgc cgggatgagc cggatgctag gcgcgaatgt
     3241 gattgccacc agcggacgtg cagttgaagc ggcaggtgac gttgacgttc tgctactgga
     3301 taaaaccggc accatcacac tcggtaaccg tcaggcgtcg gagtttatcc ccgcgcaggg
     3361 cgtggatgaa aaaacgctgg ctgacgccgc acaactggct tcgctggctg atgaaacgcc
     3421 ggaaggccgc agtattgtga tcctcgccaa gcagcgtttt aacctgcgcg agcgcgatgt
     3481 gcagtcgctc catgccacct ttgtaccgtt tactgcgcaa agccggatga gcgggatcaa
     3541 catcgacaac cgcatgatcc gtaaaggttc tgtcgatgcc attcgtcgcc atgttgaggc
     3601 taacggtggt cacttcccta ccgatgttga tcaaaaagtc gatcaggttg cgcgtcaggg
     3661 agccacgccg ctggtggtgg tggaaggttc tcgtgtgctg ggcgttattg cgctgaaaga
     3721 tatcgtcaaa ggcggtatta aagagcgctt cgcgcaactg cgccaaatgg gcattaaaac
     3781 ggtgatgatt accggcgata accgtctgac tgccgccgcc attgctgcgg aagcgggcgt
     3841 cgatgatttt ctcgccgaag cgacaccgga ggccaagctg gcattgattc gtcagtatca
     3901 ggcggaaggc cgtttggtgg cgatgaccgg cgacggcacc aacgatgctc cggcgctggc
     3961 gcaggcagat gtcgcggtgg cgatgaactc cggtactcag gcggcgaaag aagcgggcaa
     4021 tatggtcgat ctcgactcta acccgaccaa gttgatcgag gtggtacata ttggcaaaca
     4081 gatgctgatg acccgtggct cgctgaccac cttcagcatt gccaacgatg tggcgaaata
     4141 cttcgccatt attccggcgg cgttcgcggc aacgtatccg cagttaaatg cgctgaacat
     4201 catgcgcctt cattcgcccg actccgccat tctcagtgcg gtcattttca acgccttgat
     4261 tatcgtcttt ttgattcctc tggcgttaaa aggcgtgagt tataaaccgc ttaccgcttc
     4321 tgccatgttg cgccgtaact tatggattta cggtctgggt gggctgctgg tgccgtttat
     4381 tggtatcaaa gtcattgatt tactgctgac cgtttgcggt ctggtgtgag gtttaccatg
     4441 cgtggattac gtccggcatt atcgacattt atctttctgt tattgattac tggcggcgtt
     4501 tacccgctgc tgaccaccgt gctggggcaa tggtggtttc cctggcaggc caacggttcg
     4561 ttgatccgtg aaggtgatac agtgcggggt tcggcattaa tcgggcagaa ttttaccggc
     4621 aacggctatt ttcatggtcg cccgtcggca acggcagaaa tgccctataa tccacaggct
     4681 tctggcggga gcaatctggc ggtcagtaac cctgagctgg ataaactaat agccgcacgc
     4741 gttgctgcat tacgggccgc taacccggat gccagcgcga gcattccggt tgaactggtg
     4801 acggcatcgg caagcgggct ggacaataat atcaccccgc aagcggcggc ctggcagatc
     4861 ccacgcatcg cgaaagcgcg taatctcagc gttgaacagc tcacgcaact gatcgcaaaa
     4921 tacagccaac aaccactggt gaaatatatc ggccagccgg tcgtcaacat tgtcaaactc
     4981 aatctggcgc tggataaact tgatgaataa cgaaccctta cgtcccgacc ccgatcgtct
     5041 gctggaacaa actgccgcgc cgcatcgggg gaagctgaaa gttttcttcg gtgcctgcgc
     5101 aggcgtcggg aagacctggg cgatgctggc agaagcccag cgactgcggg cgcaagggct
     5161 ggatattgtg gttggcgtgg tagaaaccca cgggcggaaa gataccgccg ccatgctgga
     5221 agggctggct gttctgccgc caaaacgcct ggcgtaccgt gggcggcata tcagcgagtt
     5281 tgatctcgat gccgccctcg cccgccgtcc ggcgctgatc ttaatggacg aactggcgca
     5341 cagtaatgcg cctggttctc gtcatcccaa acgctggcag gatattgaag agctgctgga
     5401 agcaggcatt gatgttttta ctaccgtcaa cgttcaacat ctggaaagcc tgaatgatgt
     5461 ggtcagcggc gtcaccggaa ttcaggtacg ggaaaccgtg cccgatcctt ttttcgatgc
     5521 cgccgacgac gtagtgctgg tggacttgcc cccggacgat ctgcgccagc ggctgaaaga
     5581 aggcaaagtc tatattgccg ggcaggcgga gcgcgccatt gaacattttt tccgcaaagg
     5641 caatctgatc gccctgcgtg aactggcact gcgccgtact gccgatcgcg tagatgagca
     5701 aatgcgcgcc tggcgggggc atcctggtga agagaaagtg tggcacacgc gcgacgcgat
     5761 ccttttatgc atcgggcata acaccggcag tgagaaactg gtccgcgcgg cggcgcggtt
     5821 ggcctcgcgc ctgggtagcg tctggcacgc ggtgtatgtt gaaacccctg ccctgcatcg
     5881 cttaccggaa aaaaaacgtc gggcaattct cagcgcctta cgtctggcgc aggagctggg
     5941 cgcggagacg gctacacttt ctgatccagc ggaagaaaaa gcagtagtgc gttatgcccg
     6001 tgaacataat ctcggcaaga ttattctcgg tcgcccggcc tcgcgccgct ggtggcgtcg
     6061 ggaaacgttt gctgaccgac tggcgcgcat cgcccccgat ctcgatcagg tgctggtcgc
     6121 gcttgatgaa ccacccgccc gcacgattaa caacgcgccg gatagccgct cttttaaaga
     6181 caagtggcgg gtacagattc agggatgcgt ggttgccgcc gcgttatgcg ccgttatcac
     6241 tttaattgcc atgcagtggc tgatggcgtt tgatgccgcc aacctggtga tgctgtatct
     6301 gcttggcgtg gtggtggtgg cgctatttta tggacgctgg ccttcggtgg ttgctaccgt
     6361 cattaatgtg gtgagtttcg atctcttttt tatcgcccca cgcggcacgc tcgctgtctc
     6421 tgatgtgcaa tatctgctga ccttcgcggt gatgttaacc gtcgggctgg tgatcgggaa
     6481 ccttactgcg ggtgtgcgtt atcaggcgcg ggtagcccgt taccgcgagc aacgcacacg
     6541 gcacttatat gaaatgtcga aagctctggc ggtgggccgc agtccgcagg acatcgccgc
     6601 caccagcgaa caatttattg cctccacgtt tcatgcccgc agtcaggtgt tgttgcccga
     6661 tgacaacggt aaattgcagc cgttaacaca tccgcaagga atgacgccat gggacgatgc
     6721 catcgcgcag tggagttttg ataaaggcct gcctgcgggt gcgggcaccg acacgttacc
     6781 cggcgtgccg taccagattt tgccgctaaa aagcggcgag aaaacctacg gactggtggt
     6841 ggtggaaccg gggaatctgc gtcagttgat gatcccggag cagcagcgcc tgctagagac
     6901 gtttacgctg ttagtcgcca atgcgtttga gcggctgacg ctgaccgcca gtgaagaaca
     6961 ggcgcggatg gcaagcgaac gtgaacagat ccgcaacgcc ctgctggcgg cgctttcgca
     7021 tgatttacgc acgccgctta cggtgctgtt tggtcaggca gaaatcttaa cgctcgatct
     7081 ggcaagcgaa ggatcgcccc acgcccgcca ggccagcgag attcgtcagc atgtgctgaa
     7141 cactacccga ctggtgaata atctactgga tatggcgcga attcagtccg gcggctttaa
     7201 tttgaagaaa gagtggttaa cgctggaaga agtagtcggc agcgcgctgc aaatgctgga
     7261 accgggttta tcgtcgccca tcaatctttc tctgccagaa ccgctgacct taatccacgt
     7321 tgacgggcca ctctttgaac gggtgctgat taatctgctg gagaacgcgg tgaaatatgc
     7381 gggtgcgcag gccgaaattg gtatcgatgc ccacgttgag ggcgaaaatc tacaactgga
     7441 tgtctgggat aacggccccg gtcttccgcc aggccaggag cagacgatat ttgataagtt
     7501 tgctcgcggg aataaagagt cggcagtacc gggggtaggg cttggactgg caatttgtcg
     7561 ggcgatagtg gatgtacacg ggggcactat taccgcgttc aaccgaccgg aaggtggtgc
     7621 ctgttttcgt gttacacttc cccagcaaac cgctcctgaa cttgaagact ttcatgagga
     7681 tatgtgacaa acgttctgat tgttgaagat gaacaggcta ttcgtcgctt tctgcgcacg
     7741 gcgctggagg gcgacgggat gcgcgtctat gaggccgaaa cgctgcaacg cggcttgctg
     7801 gaagcggcta cccgtaagcc agatttgatt attctcgatc tcggcctgcc cgatggtgat
     7861 gggattgagt ttatccgcga cctgcgccag tggagcgcgg tgccggtgat tgtgctttcc
     7921 gcacgcagcg aagagagcga caaaatcgcc gcgctggatg ccggagcgga tgattatctg
     7981 agtaagccgt ttggcattgg cgaattgcag gcccgtctgc gcgtcgcttt acgccgccac
     8041 tccgccaccg ccgcgcccga tccgctggtg aaattttccg atgttaccgt cgatttagcc
     8101 gcccgcgtga ttcaccgggg tgaggaagag gtgcatctca cgccgattga gttccgcctg
     8161 ctggcggtgc tgctgaataa tgccgggaaa gtgctcaccc agcgccagtt actcaatcag
     8221 gtgtgggggc caaacgcggt cgaacacagt cactatttgc gtatttatat gggacatctg
     8281 cgacaaaaac tggaacagga tcccgctcgc ccccgccatt tcattactga aaccg
//
LOCUS       DAZGSP010000163         8107 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZGSP010000163 DAZGSP010000000
VERSION     DAZGSP010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.164"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..275)
                     /locus_tag="ACHK74_004744"
     CDS             complement(<1..275)
                     /locus_tag="ACHK74_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFP0223436.1"
                     /translation="MSRFIQGDCVRVMATFPGNAVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVV"
     gene            complement(352..588)
                     /locus_tag="ACHK74_004745"
     CDS             complement(352..588)
                     /locus_tag="ACHK74_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223437.1"
                     /translation="MRRLTRRGRNAACGQCPASGWARDGRFYRSRLASVTVYASPSPF
                     SDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRTR"
     gene            489..710
                     /locus_tag="ACHK74_004746"
     CDS             489..710
                     /locus_tag="ACHK74_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HFP0223438.1"
                     /translation="MPAGSGKSARPVPSRTRDTDHRQRSCRAASAGAVKRGARRHPLP
                     EKKRAVARQSPSYFIHRNAPPCRLSTPAR"
     gene            complement(661..1562)
                     /locus_tag="ACHK74_004747"
                     /pseudo
     CDS             complement(661..1562)
                     /locus_tag="ACHK74_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
     gene            complement(2214..3185)
                     /locus_tag="ACHK74_004748"
     CDS             complement(2214..3185)
                     /locus_tag="ACHK74_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HFP0223439.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(3185..4351)
                     /gene="sopA"
                     /locus_tag="ACHK74_004749"
     CDS             complement(3185..4351)
                     /gene="sopA"
                     /locus_tag="ACHK74_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HFP0223440.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(4939..5694)
                     /gene="repE"
                     /locus_tag="ACHK74_004750"
     CDS             complement(4939..5694)
                     /gene="repE"
                     /locus_tag="ACHK74_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HFP0223441.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD
                     QIRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            complement(6394..7200)
                     /locus_tag="ACHK74_004751"
     CDS             complement(6394..7200)
                     /locus_tag="ACHK74_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP0223442.1"
                     /translation="MSGSVIHSQSAAMVPAVYSAGQSAQLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
     gene            complement(7201..7506)
                     /gene="ccdB"
                     /locus_tag="ACHK74_004752"
     CDS             complement(7201..7506)
                     /gene="ccdB"
                     /locus_tag="ACHK74_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFP0223443.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(7508..7726)
                     /gene="ccdA"
                     /locus_tag="ACHK74_004753"
     CDS             complement(7508..7726)
                     /gene="ccdA"
                     /locus_tag="ACHK74_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFP0223444.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTAMQNEARRLRAERW
                     QAENQEGMAEVARFIEMNGSFADENRDW"
BASE COUNT         2147 a         2142 c         1889 g         1929 t
ORIGIN      
        1 accacgctga atcccgcatt tttccaggcg gccataaagc gatcgacacg gttccagccg
       61 tagaagctga ccatcagcgc gtcttttttg agtacgcggt acatttcatt acaggccggt
      121 tgcagccatt catcggtttt atcgccggcg atggtacgcc cctgacggtc acggaaaccg
      181 acgagatatg gcgggtcggt gaggatgaaa tcgacagcat tgcccggaaa cgtggccata
      241 acacgcacgc agtcaccctg gataaaacgg gacataacag cactccttct cttacagagg
      301 gcaccgggag ggcgcccctg tggcgcccgt tccccgcggc ctgtgggctg ttcaccgcgt
      361 ccggtaacgg gtcaggccga ccgtggaata tcgcagccgt cgtctttttg acggcgagaa
      421 tatgccgcga aagcggcttg aagggcgttc atcagaaaag ggcgacgggc tggcgtacac
      481 tgtcaccgat gccagccggg agcggtaaaa gcgcccgtcc cgtgcccagc cggacgcggg
      541 acactgacca caggcagcgt tcctgccgcg ccgcgtcagc cggcgcagta aaaagaggag
      601 cccggcgaca tcctttaccg gaaaagaaac gggcggttgc ccgccagtct ccgtcatact
      661 tcatccaccg taatgcgcca ccttgtcgac tatccactcc ggcgcggtga cttccggcag
      721 ctcatcatca tctgtcgggg aagaccattc cagttccccc cacagcacat caacaagctc
      781 tccacgctca tatcgctgcc agccacagaa attgcagccc tgctcggcgt accagtgctc
      841 cagcgtgcaa ctgaaacggc ggcttaatac tgcaataacg tcgctttccg gctgacacca
      901 cggcgtgtcg aaatcaacct gaatgaagtt gtctccctga ctgctgatat tcacctcata
      961 cccacaaggc cccttcacgc catactgttc cgtataccag tgatatgcag aaggaacacc
     1021 gttcagaaca ccaccgttaa agccattcac ttcaacatcc aggcgagtga acagaaccgt
     1081 cagcaggtca aacggcatgg tttttccggc aggacgttgt cacacagacg gttccaccac
     1141 accgatacat cctcgttact ccagatgtcg caccagtcac ctctttttgg ggtgaaaaga
     1201 gcggtaatgc tttccctggc atcatccggt aattcttccc agcgacgccg gccagtaccg
     1261 ctctgcagcc agagttcatg cagcatcagg cagttttgct catccagaag tacaccgtcc
     1321 tgcaggtgtg tcagccagcg ggtgaacgcg atattctccg gcgatacaac gccacgcccg
     1381 gcagccgtca gtccggggca tggttcaaac cgcacatctt cagtggtctg cagaagtccg
     1441 gcacttccgg ccagaaacag ctgaatacct tcatttgtgg cgcggcgata aagtggtgtg
     1501 actgcaccgc tggccagtcg tttaatctca gcgatctgtg ccggttcacc agaaaaatac
     1561 atacgattcg agcaccagtt tggcatggga attctcctct cacatgcggc ctccatcagg
     1621 ccgtgccgca cctgtaaccg ggcgaccgca tacgaatgca ggtgcggccc cgaagccgca
     1681 gcgcgtggag gaggaacgac gaaacgtgac cgggtgagga ggccggcgac agcgacacac
     1741 ttgcatcgga tgcagcccgg ttaacgtgcc ggcacggcct gggtaaccag gtattttgtc
     1801 cacataaccg tgcgcaaaat gttgtggata agcaggacac agcagcaatc cacagcaggc
     1861 atacaaccgc acaccgaggt tactccgttc tacaggttac gacgacatgt caatacttgc
     1921 ccttgacagg cattgatgga atcgtagtct cacgctgata gtctgatcga caatacaagt
     1981 gggaccgtgg tcccagaccg ataatcagac cgacaacacg agtgggatcg tggtcccaga
     2041 ctaataatca gaccgacgat acgagtggga ccgtggtccc agactaataa tcagaccgac
     2101 gatacaagtg gaacagtggg cccagagaga atattcaggc cagttatgct ttctggcctg
     2161 taacaaagga cattaagtaa agacagataa acgtagacta aaacgtggtc gcatcagggt
     2221 gctggctttt caagttcctt aagaatggcc tcaattttct ctatacactc agttggaaca
     2281 cgagacctgt ccaggttaag caccatttta tcgcccttat acaatactgt cgctccagga
     2341 gcaaactgat gtcgtgagct taaactagtt cttgatgcag atgacgtttt aagcacagaa
     2401 gttaaaagag tgataacttc ttcagcttca aatatcaccc cagctttttt ctgctcatga
     2461 aggttagatg cctgctgctt aagtaattcc tctttatctg taaaggcttt ttgaagtgca
     2521 tcacctgacc gggcagatag ttcaccgggg tgagaaaaaa gagcaacaac tgatttaggc
     2581 aatttggctg tgttgataca gcgggtaata atcttacgtg aaatattttc cgcatcagcc
     2641 agcgcagaaa tatttccagc aaattcattc tgcaatcggc ttgcataacg ctgaccacgt
     2701 tcataagcac ttgttgggcg ataatcgtta cccaatctgg ataatgcagc catctgctca
     2761 tcatccagct cgccaaccag aacacgataa tcactttcgg taagtgcagc agctttacga
     2821 cggcgactcc catcggcaat ttctatgaca ccagatactc ttcgaccgaa cgccggtgtc
     2881 tgttgaccag tcagtagaaa agaagggatg agatcatcca gtgcgtcctc agtaagcagc
     2941 tcctggtcac gttcattacc tgaccatacc cgagaggtct tctcaacact atcaccccgg
     3001 agcacttcaa gagtaaactt cacatcccga ccacatacag gcaaagtaat ggcattaccg
     3061 cgagccatta ctcctacgcg cgcaattaac gaatccacca tcggggcagc tggtgtcgat
     3121 aacgaagtat cttcaaccgg ttgagtattg agcgtatgtt ttggaataac aggcgcacgc
     3181 ttcattatct aatctcccag cgtggtttaa tcagacgatc gaaaatttca ttgcagacag
     3241 gttcccaaat agaaagagca tttctccagg caccagttga agagcgttga tcaatggcct
     3301 gttcaaaaac agttctcatc cggatctgac ctttaccaac ttcatccgtt tcacgtacaa
     3361 cattttttag aaccatgctt ccccaggcat cccgaatttg ctcctccatc cacggggact
     3421 gagagccatt actattgctg tatttggtaa gcaaaatacg tacatcaggc tcgaaccctt
     3481 taagatcaac gttcttgagc agatcacgaa gcatatcgaa aaactgcagt gcggaggtgt
     3541 agtcaaacaa ctcagcaggc gtgggaacaa tcagcacatc agcagcacat acgacattaa
     3601 tcgtgccgat acccaggtta ggcgcgctgt caataactat gacatcatag tcatgagcaa
     3661 cagtttcaat ggccagtcgg agcatcaggt gtggatcggt gggcagttta ccttcatcaa
     3721 atttgcccat taactcagtt tcaatacggt gcagagccag acaggaagga ataatgtcaa
     3781 gccccggcca gcaagtgggc tttattgcat aagtgacatc gtccttttcc ccaagataga
     3841 aaggcaggag agtgtcttct gcatgaatat gaagatctgg tacccatccg tgatacattg
     3901 aggctgttcc ctgggggtcg ttaccttcca cgagcaaaac acgtagcccc ttcagagcca
     3961 gatcctgagc aagatgaaca gaaactgagg ttttgtaaac gccaccttta tgggcagcaa
     4021 ccccgatcac cggtgggaat acgtcttcag cacgtcgcaa tcgcgtacca aacacatcac
     4081 gcatatgatt aatttgttca attgtataac caacacgttg ctcaacccgt cctcgaattt
     4141 ccatatccgg gtgcggtagt cgccctgctt tctcggcatc tctgatagcc tgagaagaaa
     4201 ccccaactaa atccgctgct tcacctattc tccagcgccg ggttattttc ctcgcttccg
     4261 ggctgtcatc attaaactgt gcaatggcga tagccttcgt catttcatga ccagcgttta
     4321 tgcactggtt aagtgtttcc atgagtttca ttctgaacat cctttaatca ttgctttgcg
     4381 tttttttatt aaatcttgca atttactgca aagcaacaac aaaatcgcaa agtcatcaaa
     4441 aaaccgcaaa gttgtttaaa ataagagcaa cactacaaaa ggagataaga agagcacata
     4501 cctcagtcac ttattatcac tagcgctcgc cgcagccgtg taaccgagca tagcgagcga
     4561 actggcgagg aagcaaagaa gaactgttct gtcagatagc tcttacgctc agcgcaagaa
     4621 gaaatatcca ccgtgggaaa aactccaggt agaggtacac acgcggatag ccaattcaga
     4681 gtaataaact gtgataatca accctcatca atgatgacga actaaccccc gatatcaggt
     4741 cacatgacga agggaaagag aaggaaatca actgtgacaa actgccctca aatttggctt
     4801 ccttaaaaat tacagttcaa aaagtatgag aaaatccatg caggctgaag gaaacagcaa
     4861 aactgtgaca aattaccctc agtaggtcag aataaatgtg acgaaccacc ctcaaatctg
     4921 tgacagataa ccctcagact atcctgtcgt catggaagtg atatcgcgga aggaaaatac
     4981 gatatgagtc gtctggcggc ctttcttttt ctcaatgtat gagaggcgca ttggagttct
     5041 gctgttgatc tcattaacac agacctgcag gaagcggcgg cggaagtcag gcatacgctg
     5101 gtaactttga ggcagctggt aacgctctat gatccagtcg attttcagag agacgatgcc
     5161 tgagccatcc ggcttacgat actgacacag ggattcgtat aaacgcatgg catacggatt
     5221 ggtgatttct tttgtttcac taagccgaaa ctgcgtaaac cggttctgta acccgataaa
     5281 gaagggaatg agatatgggt tgatatgtac actgtaaagc cctctggatg gactgtgcgc
     5341 acgtttgata aaccagggaa aagattcata gcctttttca tcgccggcat cctcttcagg
     5401 gcgataaaaa accacttcct tccccgcgaa actcttcaat gcctgccgta tatccttact
     5461 ggcttccgca gaggtcaatc cgaatatttc agcatattta gcaacatgga tctcgcagat
     5521 accgtcatgt tcctgtaggg tgccatcaga ttttctgatc tggtcaacga acagatacag
     5581 catacgtttt tgatcccggg agagactata tgccgcctca gtgaggtcgt ttgactggac
     5641 gattcgcggg ctatttttac gtttcttgtg attgataacc gctgtttccg ccatgacaga
     5701 tccatgtgaa gtgtgacaag tttttagatt gtcacactaa ataaaaaaga gtcaataagc
     5761 agggataact ttgtgaaaaa acagcttctt ctgagggcaa tttgtcacaa caccttctct
     5821 agaaccagca tggataaagg cctacaaggc gctctaaaaa agaagatcta aaaactataa
     5881 aaaaaataat tataaaaata tccccgtgga taagtggata accccaaggg aagttttttc
     5941 aggcatcgtg tgtaagcaga atatataagt gctgttccct ggtgcttcct cgctcactcg
     6001 agggcttcgc cctgtcgctc gactgcggcg agcactactg gctgtaaaag gacagaccaa
     6061 acatcatggt tctgtgttca ttaggttgtt ctgtccattg ctgacataat ccgctccact
     6121 tcaacgtaac accgcacgaa gatttctatt gttcctgaag gcatattcaa atcgttttcg
     6181 ttaccgcttg caggcatcat gacagaacac tacttcctat aaacgctaca caggctcctg
     6241 agattaataa tgcggatctc tacgataatg ggagattttc ccgactgtct cgttcgcttc
     6301 tcagtggata acagccagct tctctgttta acagacaaaa acagcatatc cactcagttc
     6361 cacatttcca tataaaggcc aaggcattta ttctcaggat aattgtttca gcatcgcaac
     6421 cgcatcagac tccggcatcg caaactgcac ccggtgccgg gcagccacat ccagcgcaaa
     6481 aaccttcgtg tagacttccg ttgaactgat ggacttatgt cccatcaggc tttgcagaac
     6541 tttcagcggt ataccggcat acagcatgtg catcgcatag gaatggcgga acgtatgtgg
     6601 tgtgaccgga acagagaacg tcacaccgtc agcagcagcg gcggcaaccg cctccccaat
     6661 ccaggtcctg accgttctgt ccgtcacttc ccagatccgc gctttctctg tccttcctgt
     6721 gcgacggtta cgccgctcca tgggtatttt cagtgttgcc accatcgtct gcagctggct
     6781 gacgtaccag gagtcagaga gcggaaccag ccggtgagtc tgctgaccgg cgggcattct
     6841 ccccgccgtc ctggcagctt tttcggtccg ttgtttcagg gtcgcaagct gcacaaacgg
     6901 atacggaggc gtgagcgaaa aatccccccg cgtcagcgcc agtgcttcat taatacgcgc
     6961 gccggtgttc cacagagtgg ccagcagcat cttgcggcgc agatccggga cataatgaag
     7021 cagggcactc acctcgggag ccagcagata tttcggcagt tcatcatgaa ccatcgacat
     7081 ctggcggagt gccagagctg ccggataatc aatggcaaca ggaagttgtg cagactgtcc
     7141 ggcagaatac actgccggta ccatggctgc agactggctg tgtataacgg agcctgacat
     7201 ttatattccc cagaacatca ggttaatggc atttttgatg tcattttccc ggtggctgag
     7261 atcagccact tcttccccga taacggagac cggcacactg gccatatcgg tggtcatcat
     7321 gcgccagctt tcatccccga catgcaccac cgggtaaagt tcacgggaga ctttatctga
     7381 cagcaggcgg gcactggcca gggggatcac catccgtcgc ccgggcgtgt caataatgtc
     7441 actctggaca tccacaaaca gacgataacg gctctctctt ttataggtgt aaaccttaaa
     7501 ctgcatttca ccagtccctg ttctcgtcgg caaaagagcc attcatttca ataaacctgg
     7561 cgacctctgc catcccttcc tgattttcag cctgccagcg ttcagcacgc agtcgccggg
     7621 cttcgttctg catggccgta ctgaccagac cggagatatt cacgtcatac gccttgagca
     7681 gctgatagct gtcgctgtca actgtcactg taatacgctg cttcatatca cacctctttt
     7741 taacatactt ctggtatgca tatcagtata tattcttatg ccgcaaaaat cagcgcgtta
     7801 atacacatac tgttatcttg cttttagtaa gccttattta ttttaccttg cattatgtta
     7861 accaataaaa attaaaatca gacttataaa aacaaagcgt aattatcgaa ggaccatttc
     7921 cggcagatcc gatttttctg ttattgacgg gtttccggaa gtgtaccacg ctgaggaaat
     7981 catcatatcg ctgttttccg cccctgcgtg gcgcatcaca ggtcagggaa ataacacaac
     8041 atatagtgtc atgagcagcc gaatgaagca ctacatgttg tacttacagg aggtggcgtt
     8101 gtgtggg
//
LOCUS       DAZGSP010000164         8047 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZGSP010000164 DAZGSP010000000
VERSION     DAZGSP010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.165"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            40..567
                     /locus_tag="ACHK74_004754"
     CDS             40..567
                     /locus_tag="ACHK74_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFP0223445.1"
                     /translation="MSNVSGIGDAYYWSVFKIAEAFGLHRDTVKKRLLAANTPVAATI
                     RGNPVYALQHVGPALFSVKHEAADSVHDPSRMEPKERKDWYQSENERIKLEKEQRKLI
                     PVDEVVIVYSSMRKAVVQVLETIPDVLERDCALTPQAVGVVQQAIDDLRYTLQEKSYE
                     ACAAELIPDEEGESL"
     gene            576..1565
                     /locus_tag="ACHK74_004755"
                     /pseudo
     CDS             576..1565
                     /locus_tag="ACHK74_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            1730..2968
                     /locus_tag="ACHK74_004756"
     CDS             1730..2968
                     /locus_tag="ACHK74_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0223446.1"
                     /translation="MSVKPLTVTEVKGMKPREKDYAVYDGFGLLLNVSKAGGKVWRFR
                     YSHPITKKRQTYTIGRFPEFSLAEAREVRDELRRMIARGVDPVTEKKNRKIEMSLKNL
                     QTFEAVANAWFAFKKGSELRKPTLYNIEYEVYKYLVPFFGKYSIEKITAPVAINALDA
                     VSDKNALQKKLISRLNEIMNYAVNCGTLKTNPLLKIKTAFTGKKNKSLAALPVERLPE
                     FLSWWDSVPHTYQIAHNALLFQILTMVRPGEAIKAEWSEIDFDSGLWIIPAHKMKCHR
                     EHVVPLSSQAISILRTMQEIKRGRYVFFSSRTKDAPMGRNTIKTPIAASKFKGIVTLH
                     GFRSMWSTLLNEEGFNPDVIEAALAHKSGDKIRDVYNRTTYLEQRKIMMQWVGDFFDD
                     ARKGVINRSGGMKGLRVVNG"
     gene            2982..3185
                     /locus_tag="ACHK74_004757"
     CDS             2982..3185
                     /locus_tag="ACHK74_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0223447.1"
                     /translation="MNTNEDILFTKDVMKILRYGAMSAFINFWKDENNGFPQPFRIGR
                     RHTWHRRDVEAWLDKQREQANPH"
     gene            3245..3823
                     /locus_tag="ACHK74_004758"
     CDS             3245..3823
                     /locus_tag="ACHK74_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFP0223448.1"
                     /translation="MNKNIAVTGKGDARHVKKFCDFRDLVVLRFDSVNVRVVYLNGDP
                     WFVAKDVCAALELTNSRTALQMLDDDEKGVNLTYTLGGNQNMSIISESGFYKLIARSR
                     KATTQGTFAHRFSNWVFRNVIPGIRKTGAYGIPWGALQDFSRRKEQYQISASEKGRAL
                     QACKRKKRELEEEEKRLIREYQPEFYFGERIQ"
     gene            complement(4216..4443)
                     /locus_tag="ACHK74_004759"
     CDS             complement(4216..4443)
                     /locus_tag="ACHK74_004759"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223449.1"
                     /translation="MTNNTLSPIQDTQTQDDEIIRQRQSEACARVEEELTRTKIPPPA
                     PRLMPPEKFALEDFVEKYPRRLKATKNRPPG"
     gene            complement(4501..4680)
                     /locus_tag="ACHK74_004760"
     CDS             complement(4501..4680)
                     /locus_tag="ACHK74_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0223450.1"
                     /translation="MERDHINNKSQKLQARAPHEVVEAMEQVKETGESTAQFIVTAMR
                     GEIKRRQRRKAKESE"
     gene            4877..5080
                     /locus_tag="ACHK74_004761"
     CDS             4877..5080
                     /locus_tag="ACHK74_004761"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0223451.1"
                     /translation="MRNHITHPQGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RQQSPAGCVMVFAARIRQGVCHA"
     gene            5073..5393
                     /locus_tag="ACHK74_004762"
     CDS             5073..5393
                     /locus_tag="ACHK74_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223452.1"
                     /translation="MPDMTNYQYLINPHFNCEHDIAKKVYSAADGATDNISMAVSSIG
                     SLMWYASENKEYDEKAMRIDMGNIGLLLAMLGQFDISLRCTIENAAEALNAIKKANTD
                     LNRG"
     gene            5400..5699
                     /locus_tag="ACHK74_004763"
     CDS             5400..5699
                     /locus_tag="ACHK74_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0223453.1"
                     /translation="MRTYLSGLTASGYAHPQIIPGAIYLDKNGNRVTVKELMFDRVYF
                     IRDGYSFLSSLNVEIFISRFRREIPLSRNNHVSCMDVDKKLQELKNMIAAWREQK"
     gene            5696..7513
                     /locus_tag="ACHK74_004764"
     CDS             5696..7513
                     /locus_tag="ACHK74_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFP0223454.1"
                     /translation="MKKAPNLKHQPRDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDS
                     VPPVWLGEQQLAELDNLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAAAGVRDADYY
                     PEGMHSQKRENWREYLERERAEQAEKKKVVELPVKKKERLKDDNASSLALNQMGASQR
                     GEVLLAHYGGELAIHADSDTVHYYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAIK
                     SAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGDFREHNKNDWLLIASELPFSPPAEG
                     ETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSGKSV
                     MAEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGG
                     DKVAIDPKHKAPYSTRIQAVVLAVNNNSMSFSDRSGGISRRRVIFNFSEVVPENERDP
                     MLAEKIEGELAVVIRHLLTRFSDQDEAKRLLYEQQKSEEALVIKREGDSLVDFCGYLM
                     SSVMCDGLLVGNAEIIPFSPRRYLYHAYLAYMRAHGFGKPVTLTRFGKDMPGAMAEYG
                     REYMKRKTKHGLRSNVTLTEDSEDWMPSCASVTNDDGKN"
     gene            7801..8046
                     /locus_tag="ACHK74_004765"
     CDS             7801..8046
                     /locus_tag="ACHK74_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223455.1"
                     /translation="MTTKHAKKPQSHALDLTEHWLRVAIKIIDRNAGEGYAKAHPELI
                     SAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVES"
BASE COUNT         2249 a         1665 c         2093 g         2040 t
ORIGIN      
        1 taaataaaca tgattttcac ctgccagatg gagttgctta tgtcaaatgt gagcgggatc
       61 ggtgatgctt attactggag tgtttttaaa atcgctgagg cctttgggct tcaccgggac
      121 acagtaaaaa aacggctcct cgcggccaac actcctgtgg ctgcgactat cagggggaac
      181 cccgtttacg ccctgcagca tgtcgggcca gccctgttta gtgtgaagca tgaggcagca
      241 gactctgttc atgatccatc ccgtatggag ccgaaagaga gaaaggactg gtaccaatct
      301 gaaaatgaaa ggatcaagct ggaaaaggag cagcgaaaac tcatccccgt tgatgaagta
      361 gtcatcgtct attcgtccat gagaaaggct gtcgtccagg ttctggagac aattccggat
      421 gttcttgaac gcgattgcgc cctgactcct caggccgtcg gcgttgtaca gcaggccatt
      481 gatgacctgc gatacactct tcaggaaaaa tcctacgagg cttgtgctgc tgaattaatt
      541 cctgatgagg aaggagagag tctctaggag gaataatggg tttttcatca gcccgaaatt
      601 tgggaaggga catatcggca ggattttccc caccacgtcg catgccgatt tcggaggctg
      661 ttaaaaaatt catgcgtgtt cccaaggggg ctggtaactc ggtgccatgg gatcctgaac
      721 tgacacccta catcattgag cccatgaact gcctggcatc gcgtgaatac gatgcggtga
      781 tttttgttgg tcctgcgcga acagggaaga ccattggtct gatcgatgga tggattgtct
      841 ataccatcgt ttgcgatcct tcggacatgc tcgttgtgca gatgaccgaa gataaggccc
      901 gcgagcattc taaaaagcgc ctcgacagaa cgttcagaag cagtgcggcg gtaaagaaaa
      961 gaatgagtcc acgtcgtaac gacaataatg tccatgataa gacgttcagg gatggctcgt
     1021 tccttaaaat tggttggccc tcggtcaaca ttatgtcgtc gtcggattac cggtttgtcg
     1081 ccttaaccga ttacgaccgt tttccggaga atatcgacag cgagggtgat ggtttctccc
     1141 tggcctcaaa acgtaccacc acatttatgt ccgccgggat gactctggtg gagagctcgc
     1201 cgggacgtga catctgcgac agcaaatggc gacgtaagtc gcctcatgaa gcgccaccga
     1261 cgactggtat tctttccctt tacaatcgtg gtgaccgccg ccgctggtac tggtcatgtc
     1321 cgcactgcgg tgaatatttt cagccagcca tggatgccat gaccggctac cgtaatgaac
     1381 cggatccctt taaagccagt gaggcggcgt atctactttg cccgcactgc agcggcatta
     1441 tcactgcgga gaaaaagcgt gagctcaata gtgcaggagt ctggttgcgt gaaggtcagg
     1501 tcattgatcg taacggcaac gtttccggtg aaccgcgccg ctcccgtatc gccagtttct
     1561 ggatgtaggg tacatttctt acctgtttgt atgttctggt gtcgtttcat agtcttttca
     1621 atgagttgtg attttatgag tttcctctct ttgcttgata atgagttagt ttatcgcttg
     1681 ttattggctt gaatggacta catgacggac taaaaaatga gggcgataga tgtcggtaaa
     1741 gccattaacc gtgactgaag ttaaggggat gaaaccacgt gaaaaggact atgccgttta
     1801 tgatgggttc ggtttattgc tgaatgtgag taaagccggt gggaaagtgt ggcgtttccg
     1861 ttatagccat ccgataacga agaaacggca gacatacacg ataggacgtt ttcctgaatt
     1921 ctcactcgcg gaagcacggg aagtacgtga tgaacttcgg cgaatgattg cacgtggagt
     1981 tgatccagtg acggagaaga aaaatcgtaa aattgagatg tcactaaaaa atctacagac
     2041 atttgaagct gttgctaatg catggttcgc ttttaaaaag ggatctgaat tgcggaaacc
     2101 tacgctgtat aatatcgaat atgaagtata caaatatctt gttcctttct ttggtaagta
     2161 cagtatagaa aaaattacag caccagtagc tattaatgct ctggatgccg tatccgataa
     2221 gaatgcgttg caaaaaaaat taatatcaag attaaatgaa attatgaatt atgctgtaaa
     2281 ttgtggaaca ttgaaaacaa atccattact taagataaag actgcattca caggaaagaa
     2341 aaataaatca ttagcagcac tacctgttga aagattgcct gaatttctga gctggtggga
     2401 tagtgtgcct catacctatc aaatagctca taatgcactt ttattccaga tattgacaat
     2461 ggtcaggcca ggtgaggcga ttaaagcaga gtggtcagag attgattttg attctggctt
     2521 gtggattatc cccgcgcata aaatgaaatg ccatcgtgaa catgttgttc ccttgtcatc
     2581 acaggctatt agtatcctca gaacaatgca ggaaataaaa agagggcgtt atgtgttttt
     2641 ttcctccaga acaaaagatg cgcctatggg gaggaatact atcaagaccc caattgctgc
     2701 cagcaagttc aaagggattg taacgttaca tggttttcgt tcaatgtgga gtacgctttt
     2761 aaatgaggag ggatttaacc ccgatgtaat cgaggctgca ttggcgcata aaagtggtga
     2821 taaaataaga gatgtttata atagaactac ttatctagaa cagcgtaaga tcatgatgca
     2881 atgggtcggt gatttttttg atgatgcgag aaaaggggta attaatagat ccggtggtat
     2941 gaaaggttta agagtagtaa atggttgagg aggttcagca aatgaatacc aatgaagata
     3001 ttttatttac taaagacgta atgaaaatct tgcgatatgg agcaatgagt gcattcatca
     3061 atttctggaa agatgagaat aatggttttc ctcagccgtt cagaattgga cgacgacata
     3121 cctggcaccg tagagatgta gaagcatggt tagataaaca acgagaacaa gccaatcccc
     3181 actaataata tctttcatac cccgcgtgca atgcggggtt ttttgtatgt gaggtaaaaa
     3241 agaaatgaat aaaaatattg ccgtgacggg caagggtgac gcacgtcatg taaaaaaatt
     3301 ctgtgatttt cgtgatctgg tcgttctgcg ctttgatagt gtgaacgttc gcgtggtgta
     3361 tctgaacggc gatccgtggt ttgttgcaaa ggatgtctgc gctgcgctgg aactgaccaa
     3421 ttcgcgtacg gcgttgcaga tgcttgatga tgatgaaaag ggagtaaatt taacttacac
     3481 cctaggagga aatcagaata tgagcattat ctctgagtca ggtttctaca aactaatagc
     3541 ccgtagccgc aaagcaacga cgcaaggcac attcgcccat cgtttcagta actgggtatt
     3601 cagaaatgtg ataccgggta tcagaaaaac gggggcttat ggtatcccgt ggggtgcatt
     3661 acaggatttt tcccgccgta aagagcaata tcagataagt gccagcgaga aggggagggc
     3721 gctacaggca tgtaagcgca aaaaacgtga actggaggaa gaagaaaaaa ggctgatacg
     3781 tgaatatcag cctgagtttt actttggtga gcgtattcag taaccacacg cggtgctgat
     3841 tatacggtac atcgtgttaa ccgagaagct acccaccagc aaggcaaaat cttctgctaa
     3901 aaaatgacat atggcccgtc gtccggaaag catgaaattt tacaaaaatg gaaaatgaag
     3961 atttttattg tgctggtggg taaaaacaaa aagcgccccg ttgccggagc gcccttgcga
     4021 acaattaacc tgctgcgaaa aaattggatc agtgcagggg aattatatca actgtgtgaa
     4081 gaagcgccac aattgccgga taacaggcaa agaaaaggcc acctgtcacg gtggcccttc
     4141 gacacaaatt tcgcgttatc cccaacgcat gagcatcgcc gacaatgcca catttacggc
     4201 tggtggacaa ggctatcagc caggcggtcg gtttttagtg gcttttaacc gccgtgggta
     4261 tttctcgaca aaatcttcaa gggcaaattt ttctggtggc attaagcgcg gcgctggtgg
     4321 tggtattttt gttctggtta gttcttcctc aactctggcg caggcttctg actgcctttg
     4381 ccggatgatt tcatcatctt gcgtttgcgt gtcttgtatt ggtgatagtg tgttgttggt
     4441 catggtactg ccctgtaaag caatgcgccg tagtacctca caccacggcg ctgatagtga
     4501 ttattctgat tctttggcct tacggcgctg gcggcgtttg atctcgcctc gcatggctgt
     4561 aacgataaat tgcgcagtgc tctcgcctgt ttcttttact tgctccatag cctcaacaac
     4621 ttcatgcggg gcgcgagcct gtaatttctg tgatttgtta ttgatatggt ctctttccat
     4681 ttctggttcc ctttgtgatt actggaatcc agtatacacg aaaaagacca aaacaaaacg
     4741 cttgaagtgg attccactta gtgattatac tggaatccag ctgagtgttt atcactcaat
     4801 gcaaacgagg ctgaaaagga ttgccgtcct ttcgcagcct ctgaccacca acgatagcga
     4861 aagtatcgag gtagctatga gaaatcatat cacacacccg caagggcggg actcgcacaa
     4921 cctgaataaa tacatctggc gttttatcgc cctgagcact gcacaaccgc gcgtgattac
     4981 cattgaggcc accagcgaac aggaagcacg ccagcaatcc ccggctggct gcgtgatggt
     5041 attcgccgcc cgtattcgtc agggggtgtg ccatgcctga catgaccaat taccagtacc
     5101 tgattaatcc gcattttaac tgtgagcatg atattgctaa aaaggtttat tccgctgcgg
     5161 atggggctac tgacaatata tcaatggctg tttcgtcaat tggtagcctg atgtggtatg
     5221 cgtcagaaaa taaggaatat gacgaaaagg ccatgcgcat tgatatgggt aatatcggtt
     5281 tgttactggc aatgcttgga cagtttgata tttcgttacg gtgcaccatt gaaaatgccg
     5341 cagaagcatt aaatgctata aagaaagcga atactgattt aaatcgggga taaataatca
     5401 tgagaacata tttatctggc ttgactgcca gcggttatgc acacccccaa attatccccg
     5461 gcgctattta tctggataag aacggtaaca gagtaacggt aaaagaactg atgtttgacc
     5521 gtgtgtattt tatccgtgat ggctattcat ttcttagttc gctgaacgtg gagatcttta
     5581 ttagcagatt caggcgggaa atcccgcttt ccagaaataa ccatgtgtca tgtatggatg
     5641 ttgataaaaa actacaggaa ctaaaaaaca tgattgccgc gtggagagag cagaaatgaa
     5701 aaaagcgcca aatttaaaac accagccacg tgacaaaatg acggaagtca tcatttttgc
     5761 gggtagtgat gcgtgggcac atgcgaagca gtggcaggaa caggacgggc gactggctgg
     5821 cgacagcgtg cctcctgtct ggcttggaga gcaacaactt gccgaactgg acaacctgca
     5881 aatcgtaccg gacggacgct atcgcgtgcg tctctaccag gcggggttat tgcgtccggg
     5941 gcttgttaat accatcgggc agaaactggc agcggcaggt gtcagggatg ctgattatta
     6001 ccctgaagga atgcacagcc agaaacggga gaactggcgc gaatatctgg aacgtgaacg
     6061 ggcagagcag gcggaaaaga aaaaggtagt tgaactgcct gtaaagaaaa aagagcggct
     6121 aaaagacgat aacgcttcat cactggcgct taaccagatg ggagcaagtc aacgcggcga
     6181 agttctcctg gcacattatg gcggtgaact ggcgattcat gctgactctg acactgttca
     6241 ctattacaac ggcgttgtat gggagccagt acaggataaa gaattacagc gagctatggc
     6301 acagattttc attgatgcgg agatcagcta ttcgcagaac gccattaaat cggcggtcga
     6361 taccatgaag ttaagtttgc ctgtaatggg gaatacagcc cgtaacctga ttggattcag
     6421 taacggtgtt tttgataccc ggacaggcga ttttcgggag cataacaaaa acgactggtt
     6481 gttaattgcc agtgaattac ctttcagccc accagcagag ggggaaacgc tggcaacaca
     6541 tgcgccgaat ttctggaagt ggttacgccg ttcggtggct gagaatgacc gcaaggcgga
     6601 tcgcgtactg gctgcattat tcatggtgct ggcgaaccgg tacgactggc agttattcat
     6661 tgaggtaaca ggtccagggg gaagtggtaa aagcgtgatg gcggagattt gcaccatgct
     6721 ggcgggtaag gccaatacgg tatcggcaag catgaaggcg ctggaagatg ccagggaacg
     6781 cgcgttagtg gttggctttt cgctgattat catgccggat atgacccgtt acgctggtga
     6841 tggcgcagga attaaggcca ttacaggcgg tgacaaggtg gcaatcgacc cgaagcataa
     6901 agccccctac tcaacacgca ttcaggcggt agtgttggcg gtgaacaata actccatgtc
     6961 attcagtgac cgcagcgggg ggatttcacg tcgtcgggtg atattcaatt tttcggaggt
     7021 tgtaccggaa aacgaacgcg accccatgct ggcagaaaaa atagaaggag agctggcggt
     7081 tgtgattcgc cacctgctta ctcgtttttc tgaccaggac gaagctaaac gcctgctgta
     7141 tgagcagcag aaatcagaag aagctctggt gataaaacgc gagggcgatt cgctggtgga
     7201 cttctgcggc tatctcatgt cgtcggtaat gtgtgatggt ctgttagtgg gtaatgccga
     7261 aattattccg ttcagtccgc gcaggtatct ttatcacgcc tatctggcat atatgagggc
     7321 acacggattc ggtaaacctg taacactgac gcgcttcggt aaagatatgc cgggggcaat
     7381 ggcggaatat ggcagggagt atatgaaacg gaaaacgaag cacggtttgc gttcaaatgt
     7441 gaccctgaca gaggattcag aagactggat gccatcatgt gcatcggtca caaatgacga
     7501 tggaaaaaat taaacttatg gaataactgt tcaccactgt tcaccctgca ataaatgtct
     7561 tttatatcag tatattatag ggtgaacagt tatttatgaa ctgttcacca aactattcac
     7621 tgttcacctt tttgattctt tattgagctt taagggtgaa cagtggtgaa cagttggtga
     7681 atagtttttg tgaaactgtt caccccttaa cattatgaat aaaaagggaa aattgcaaaa
     7741 ggtgaacagg tgaagggttg aaatgcaaaa attttatttt attgctgtga ggtaaagcct
     7801 gtgacaacga agcacgccaa aaaaccacaa tctcacgccc ttgatttgac agaacactgg
     7861 ctgagggtgg cgataaaaat catcgaccgc aacgccgggg aaggatacgc gaaagcacat
     7921 cccgaactga taagcgcatt catgaccaca gcggcggcaa actttgccac gctgacagaa
     7981 cgggagattg ccgaagcgga acaggtaaca accatcaacg ttaaaaccgg agaggtggaa
     8041 tcatgac
//
LOCUS       DAZGSP010000165         8021 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZGSP010000165 DAZGSP010000000
VERSION     DAZGSP010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.166"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..459
                     /locus_tag="ACHK74_004766"
     CDS             <1..459
                     /locus_tag="ACHK74_004766"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP0223456.1"
                     /translation="LPNESDYLKLQALFARVAEEKHQRGELEKPHHQLLETYTSLNRQ
                     YAELQSEYKHLRRYFGVTAQVPYTDVWTHKPVQYYPGKHPCEKPAEMLQQIISASSRP
                     GDLVADFFMGSGSTVKAALALGRRATGVELETERFEQTVREVQDLVSQNG"
     gene            500..575
                     /locus_tag="ACHK74_004767"
     tRNA            500..575
                     /locus_tag="ACHK74_004767"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:533..535,aa:Ile,seq:cat)
     gene            585..661
                     /locus_tag="ACHK74_004768"
     tRNA            585..661
                     /locus_tag="ACHK74_004768"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Arg,seq:tcg)
     gene            940..1368
                     /gene="tolA"
                     /locus_tag="ACHK74_004769"
     CDS             940..1368
                     /gene="tolA"
                     /locus_tag="ACHK74_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFP0223457.1"
                     /translation="MYCRAVPVLVLILASLTTGCTKDVSTNNYDASLYHSDKLIKSQN
                     LSSSERTPDASEEIRQYAIQVRKAIEEQLKDASKYSGKECSLRMYMAPNGLLLQVKRE
                     SGDPDLCREAMNAVKNADIPSPPSSGVYKAFRNGVLDFKL"
     gene            1837..2001
                     /locus_tag="ACHK74_004770"
                     /pseudo
     CDS             1837..2001
                     /locus_tag="ACHK74_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483513.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
     gene            2065..2790
                     /locus_tag="ACHK74_004771"
     CDS             2065..2790
                     /locus_tag="ACHK74_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0223458.1"
                     /translation="MDVACSVVLIRYPIDIFLEKEKVSLDAGSVMLVARNIRGLFCAY
                     ADRVKMADISNSVVNQYLERECELTEYTPTKMPLYLMSDIYNTELAEALITRHSESSD
                     TLKDFSVITAFSCISLFATDRRLPLFLSGAVNNISCKVRAIIQTDISAGWMLGVIALQ
                     LHMSESLLKRKLKDEGYSFSRLLLEERMRVAVNLVCFQRECGQAIAEKCGYSSKSYFI
                     SVFHQYYGAPPESYAYLQKADVI"
     gene            complement(4055..4140)
                     /locus_tag="ACHK74_004772"
                     /pseudo
     CDS             complement(4055..4140)
                     /locus_tag="ACHK74_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4407..6371
                     /locus_tag="ACHK74_004773"
     CDS             4407..6371
                     /locus_tag="ACHK74_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP0223459.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDAGGAPENSARWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFTVVWMQGEFDFDGTPGNHAAQFGALVDKFRADLTD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSTWARRGIISDRLATAIL
                     VHAGRTAEFITGKQPDMVKPTVPSGEGPEREAEAPVSNRTLMSLLASGEDLASQGWRY
                     YHKPAGGDSVNKNIAEAVVSDAGATGGKALQLNKPENHIWFLEHDAAGQGAELLKKGG
                     RVSVRFKLPGSLVPNQFALGIYWQLSSLPEGVTLSGEGNDMLMSFFLQTDTTNLNAMH
                     HRKPNAKLDTFGVFDNGWHTLAFEFAGNNSIQVTPVLDEKRGAAFTLVKSPASGAADK
                     LQLTDISKSATYTLLIDSIAVEVNSTDTAA"
     gene            6506..6685
                     /locus_tag="ACHK74_004774"
     CDS             6506..6685
                     /locus_tag="ACHK74_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0223460.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            6726..6998
                     /locus_tag="ACHK74_004775"
     CDS             6726..6998
                     /locus_tag="ACHK74_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP0223461.1"
                     /translation="MSEITSLVTAEAVKDVLRSEEVLSALKQKLRHKLEARLDAEVDA
                     ILDELLGVQAEPATEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            7074..7289
                     /locus_tag="ACHK74_004776"
     CDS             7074..7289
                     /locus_tag="ACHK74_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP0223462.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            7293..7631
                     /locus_tag="ACHK74_004777"
     CDS             7293..7631
                     /locus_tag="ACHK74_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFP0223463.1"
                     /translation="MNKQYELVVKGINNYPDKITVTVALETGGYTSLLLPNVVIDLDR
                     VEGAPLEFYEAEAKKKAKQFFMDIAAGLCEGNEPLLEKRPIILEAQNVLITYKGKLPG
                     RITDSLKVRI"
     gene            7668..>8021
                     /locus_tag="ACHK74_004778"
     CDS             7668..>8021
                     /locus_tag="ACHK74_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFP0223464.1"
                     /translation="MNTKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKGKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKC"
BASE COUNT         2051 a         1695 c         2266 g         2009 t
ORIGIN      
        1 ctaccgaacg aaagcgatta tctgaaatta caggcgctgt ttgcccgggt ggcagaagag
       61 aagcatcagc ggggtgaact ggaaaagccc caccaccagc tgctggagac gtatacttca
      121 ctgaaccggc agtatgcgga actgcagagt gaatataagc atctgcggcg gtattttggc
      181 gtgacggcgc aggtgccgta cacggatgtg tggacgcata aaccggtgca gtactatccc
      241 gggaaacatc cgtgcgaaaa gccggcagaa atgctgcagc agataatcag cgcgagtagt
      301 cgtccggggg acctggttgc agattttttc atggggtcgg gttcgacagt taaagctgca
      361 ctggcactgg gccgtcgtgc aactggcgtt gagctggaga ctgaacgttt tgagcagacg
      421 gtcagggaag tacaggattt agtcagtcag aatggatgat attgcaggat tagttacgta
      481 ccgttattat cctgctcccg gccctttagc tcagtggtga gagcgagcga ctcataatcg
      541 ccaggtcgct ggttcaaatc cagcaagggc caccatatca cataccgcca ttagctcatc
      601 gggatagagc gccagccttc gaagctggct gcgcggggtt cgagtcctcg atggcggtcc
      661 attatctgca ttatgcgttg ttggttctga tgagtgaatc attgtctttt gagcaacagg
      721 ctatcatata agatagcctc agacttcctt gtttattctc gccggatgct tcgtagatat
      781 ttgcaggcat ctgtatgcag gggtattatt gcggcatata tcatccggaa aaataaaaac
      841 tacatgctga tccggagatg atagttacaa gtcctcccgg atcccgcata tttactatat
      901 tttatagata attgttcttg ttaatatgga gatggataaa tgtattgccg tgctgtaccg
      961 gttcttgttt tgatattagc cagtctgaca actggctgca caaaagatgt atcgacaaat
     1021 aattatgatg cttctctgta tcactcagat aaattaataa agtcgcaaaa tttgagctca
     1081 tcggaacgta cccctgatgc cagtgaagaa atccggcagt atgccattca ggtcaggaaa
     1141 gccatagaag aacagttaaa ggatgcgagt aagtattcag gaaaagagtg ttcactgaga
     1201 atgtatatgg ccccgaatgg tcttctgcta caggttaaaa gagaaagcgg tgatcccgat
     1261 ttatgtcgtg aagcgatgaa tgcagtaaag aatgctgata taccatcacc accgtcttca
     1321 ggagtatata aagcatttag aaatggcgtg ctggatttta aactctgaca ggggggagaa
     1381 gattttctcc tgtcctgatg attttggggc gctggaaaga tattgtgctg tgaagtgtta
     1441 aatccctcac aattcagtaa gttgacagtt gcctgtcaga ctgagcattt gttaaaaaaa
     1501 tttcgcatgg tgaatccccc tgtgcggagg ggcgactggt gaacggtatg atctctttga
     1561 tgatcgtaag cgagaatacg cgggtttggt gtcaccgggc tgaactcacc gggaggcacc
     1621 cggcaccatg ctcatggtga tacagaaatg cggcttcagc ccctctccgg aggggctttc
     1681 ttatggacaa aaaaagcccg cgctgggaga cgcgggcggc aaggaataaa caataaaacg
     1741 tgaagtaata cttcaactgg cgaataatac ccgacagtaa tcactctgcg caatagcgtg
     1801 gcctttttcg aattgcgggc tgtagtctcc cttctgccgg gtgtcctggt tgcagatttc
     1861 ttcatggggt agggttcggc agttaaagca gcgatggcgc tggggcgtcg tgcaactggc
     1921 gttgagctgg ataccgggcg ttttgaacag actgcgcagg aaatcaggga tgtatttagc
     1981 gggagcggtt tgcagaagta atctcccaaa atgccatttt tggaatatac tggtattaag
     2041 tctttttgct aaggttaatt caaaatggat gttgcctgct ctgtggtcct gattcgctat
     2101 ccgatagata tttttcttga aaaagaaaaa gtttcactcg atgccggaag tgttatgctt
     2161 gttgccagaa atataagagg gcttttctgt gcttatgccg acagagtgaa aatggcagat
     2221 ataagtaaca gcgtcgtcaa tcagtatctg gaaagggagt gtgaactgac ggagtataca
     2281 ccgacaaaaa tgcctcttta tttgatgtct gatatttaca atacggaact ggcggaggct
     2341 ttaataaccc ggcatagtga atccagtgat actttgaagg atttctctgt tataaccgca
     2401 ttttcatgta tctccttatt tgcaacagac agacgtctgc cattattttt gtccggtgct
     2461 gtaaataata tcagctgtaa agtaagggct ataattcaga ccgacatatc ggcaggctgg
     2521 atgcttggtg tgattgcatt acaattgcat atgagcgaaa gcttgttgaa gcgaaaactt
     2581 aaggatgaag gttatagctt tagtcggttg ttgctggagg agcgaatgcg tgttgctgtt
     2641 aatcttgtat gtttccagcg agagtgtgga caagcgatag cggaaaaatg tggttactca
     2701 agtaagtcct acttcatttc tgtatttcac cagtattatg gtgcaccacc tgagagctat
     2761 gcatatttgc agaaagcaga cgttatctga tgttttttca tcaggcatat attgattgac
     2821 tgtattagat atagtctgaa tgtgttcagc ataaaaagtt ataatcattt ggtgtttgcg
     2881 tgaatattga tgagaaaatc agatgtagcc aattgttaca gcgtattgaa gccgtcaatg
     2941 tggaacgaac aaaggtcttc agtcgtctga cagttttatt ttgtacccct gatcgacgtt
     3001 caggtcagga gatggttctt ctggatgtag atgcacttcg gaacgtctgt gaggagttca
     3061 ccgcggctaa ttcggagtta ctgagtcttg ttcaggagta caacagaatt gcaggtagta
     3121 acggttttga tgaaatcaaa attatatccc gtggataaaa catatcctgt atatttatgt
     3181 tgttacatac aggcgctttt agtatctgcc gcacggcagt tgtacttcag aataagatgc
     3241 ttttgtgact ggtggtgttg tatctggggt tagccaggag gaggttcaga gagtccggtg
     3301 gtaattatgt gtatgcagtg gttgttgctg taccgtgggc aagttctgga actggcttcg
     3361 tccattgtag atgattgtta gctggatatc agttacctca tggaaatgtt aaatgactca
     3421 caattcggtt gatggactat tgtctgatcg atggaagtct tgtaaaaacg agccagcata
     3481 gtgaatcccc ctgtgcggag gggcgtctga catggtctcc atagcgatgg tcacaatacg
     3541 cagattcagt agtcaggctg aatctaccgg gaggcacccg gcactatgct gcttatggtg
     3601 tatacatagc gatcggcccc tctccggagg ggtttttttt gtgcgggaaa aggctgtgtt
     3661 gttaagtgtt aaattcctca cagttcagat ggttgatgct tgcctgtctg agggggggtt
     3721 gttaaaaaag ttctgcatgg tgaatccccc tgtgcggagg ggcgactggt gtgatttgca
     3781 tcttttctgt tgtaggcaat gcaaacgcgg gttcagggac accagactgg actcaccggg
     3841 aggcacccgg caccatgttc atgatgatac agatacgcag tgtcagcccc tctcctgagg
     3901 ggctttttta tgcaggaaaa acgctgtgtt gtgaaatgtt aaattcctca cagttcagat
     3961 ggttgatgct tgcctgtctt acggggagtt gttaaaaaag ttctgcatgg tgaatccccc
     4021 tgagcggagg ggcatatcag cgcaggtgtt tctactctat cctttctgtg cgggttcagg
     4081 tgctgatact gaactcaccg ggaggcaccc ggcaccatgc agtatacaga gattaggcat
     4141 acatccaggc tcctcatcgc aggagccttt ttacatgtaa aaaaaaaacc gctcctggga
     4201 agagcggttg gcaaggaagc aaggaataaa taacaaaacg tgaagtaatt tattcagcac
     4261 cctaataata ccttagagta atcaccttgc gcaactgtaa gggcgtattt cttttttgcg
     4321 ggctgttttt ctgtgtgact tttgtgtttc cggaggtcag cccgtgcctt ctctgactca
     4381 gaacattatc ccggccggga ggattcatgg catttaaaca ctacgatgtg gtcagggcgg
     4441 catcgccgtc agaccttgct gaacgactga cacaaaaact gaaggagggt tggcagccat
     4501 ttggcagtcc ggtggccatc acgccttata ccctgatgca ggccattgcg gcggaaggtg
     4561 atgtcaccac accagtggcg gtgaccggta atgagggtaa ggcggtggct gtcagtgcca
     4621 ccagcgaccc ggagtattac tttgttgtgg ttctggcagg gcagtcaaat ggcatgtcgt
     4681 atggtgaagg tcttccgctg ccggagacat atgaccgtcc ggacccgcgt attaagcagc
     4741 tggcgcgccg cagtacggtg acaccgggcg gtgcagcatg caaatataac gacatcattc
     4801 cggcggacca ttgtctgcat gatgtgcagg acatgagccg tcttaaccat ccgaaagcgg
     4861 acctgtcaaa agggcagtac ggtaccgtgg ggcaggggct gcatatcgcc aaaaaactgc
     4921 tgccgtttat accggcgaat gcgggcattc tgctggttcc gtgctgtcgt ggtggttcag
     4981 cgttcaccac cggagctgat ggcacataca gtgacgctgg cggtgccccg gagaattcag
     5041 cgcgctgggg tgtggacaag ccgctgtata aggaccttat cggtcgaaca aaagcagcac
     5101 tgaagaagaa cccgaaaaat gtgctgttta ccgtggtgtg gatgcagggg gaatttgatt
     5161 ttgacggcac gcccggaaat cacgcagcac agtttggtgc gctggttgat aaattccgtg
     5221 cagacctgac ggatatggca ggtcagtgcg ttggtggctc tgctggcggt gttccctgga
     5281 tatgcgggga cacgacgtat ttctggaagc agaagaacga atccacgtac cagacggtgt
     5341 acggcagcta taaaaacaaa acggaaaaga atatccattt cgtaccgttc atgacggatg
     5401 agaacggggt gaatgtgccg acgaacaaac cggaagaaga cccggacatt ccgggtatcg
     5461 ggtattacgg ttcgaaatgg cgtgacagct cagccacctg gacgtcacag gacagggcga
     5521 gccatttcag cacctgggca cgccggggga ttatttccga ccgtctggca acggcgattc
     5581 tggtgcacgc cgggagaacc gctgaattca ttaccggaaa acagcctgat atggtgaagc
     5641 ccaccgtacc ttccggtgaa ggtccggaga gagaggcgga agccccggtc agtaaccgaa
     5701 ccctgatgag tctgctggca tccggcgaag acctggcatc acagggctgg cgctattatc
     5761 acaaaccggc gggcggagac agtgttaaca aaaacattgc tgaagcggtg gtcagtgatg
     5821 cgggggctac gggaggtaag gccctgcaac tgaacaaacc ggaaaaccac atctggtttc
     5881 tggagcatga tgcagccgga cagggtgcgg agttactgaa gaagggcgga cgtgtgagcg
     5941 tacggtttaa gttgccgggt tcactggtgc cgaatcagtt tgccctgggc atttactggc
     6001 agttgtcgtc cctgccggag ggggtgacgc tgtcaggaga aggtaacgat atgctgatgt
     6061 ctttcttcct gcagacggat acgacgaacc tgaacgcgat gcatcacagg aagccgaatg
     6121 cgaagctgga tacattcggg gtctttgata acggatggca cacgctggct tttgagtttg
     6181 ccggaaacaa cagcattcag gtgacaccag tactggatga gaaacgggga gcggcgttca
     6241 cactggtgaa gtcaccggca tcgggggcgg cggacaaact gcaactgacc gatatatcaa
     6301 aatcggcgac gtatacgctg ctgattgaca gcattgcggt ggaagtgaac agcacagaca
     6361 ctgcggcatg ataaaaaaag ccgccagtta ccggaatgga ctggcggtgg taatacccat
     6421 ggagaaatat aaaggaacga tactttcgtg ctctgttttt ttaaatgaaa acagttctta
     6481 ttgtcaacaa taacggtaag aaattatgac ttttattcat caggtgatgc tgtacttctg
     6541 tacggcagtc tgtgtgctgt atctcctttc gggtggatac cgggccatgc gtgacttctg
     6601 gcgcagacag attgacaaaa gggccgctga gaaaatcagc gccagtcagt cagccggaag
     6661 caaacccgaa gagccgctca tttagcggca actttcttaa tcacaccttt cgacgagaaa
     6721 atcccatgtc agaaattaca tccctggtca ctgctgaagc agtgaaggac gtcctgcgct
     6781 ctgaagaagt cctgagcgca ctgaaacaga aactccgcca taagctggaa gcacgtcttg
     6841 atgcagaagt ggatgccatt ctggatgagc tgcttggtgt acaggcagag ccagcgactg
     6901 aagcgggaga taccaccgca gagagcggtg aagttcagcc tgaatcaccg gtcgccgatg
     6961 cgactgaacc gcaacccgaa tcggtcatga tgctgtaacg ggggtcaggg ccatcagtaa
     7021 actgctgctg gcctttttca tgttgtgagc ttccggattg cgggagacgg ggtatgtacc
     7081 agatggaaaa aatcacaaca ggtgtgtcat acaccacgtc agcggtggga acgggctact
     7141 ggttcctgca gttgctggac agggtttccc cgtctcagtg ggcggcaata ggcgtgctgg
     7201 ggagtctgct gtttggtctg ctgacatatc tgacgaacct gtatttcaaa attaaagaag
     7261 accggcgtaa ggcggcgcgg ggagagtaga cgatgaataa acaatacgaa ctggttgtta
     7321 aagggataaa taattacccg gataagatta ctgttactgt ggcgctggaa acgggtgggt
     7381 atacgtcact gttgttgcca aatgtggtga ttgatcttga tcgtgttgaa ggtgccccgc
     7441 tggagtttta cgaagctgag gcgaaaaaga aggcgaagca gtttttcatg gatattgctg
     7501 ccgggttatg tgaagggaac gaaccgttgc tggaaaagcg ccccataatt ttagaggcgc
     7561 aaaatgtgtt gataacctac aaaggaaagc taccgggaag aattactgat tctctgaaag
     7621 tgcgtattta gtgggcctgg gacagcggct gaatatttaa tatatccatg aacacaaaaa
     7681 taaaatacgg cctgtcggct gccgttctgg cgttgattgc cgcaggggct tctgcgcctg
     7741 aaatcctcga ccagtttctt gacgaaaaag aaggtaacca cactacggca taccgtgatg
     7801 gtgcggggat ctggaccatc tgccgtggag ccacccgggt ggatggtaag cctgttattc
     7861 ctggcatgaa gctgtcgaag gggaaatgcg accgggttaa cgccattgag cgtgataagg
     7921 cgctggcatg ggtggagaaa aacatcaaag tgccactgac tgaaccccag aaagcgggta
     7981 ttgcgtcatt ctgtccttac aacattggcc cgggtaagtg t
//
LOCUS       DAZGSP010000166         7991 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZGSP010000166 DAZGSP010000000
VERSION     DAZGSP010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.167"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(181..1374)
                     /gene="tyrB"
                     /locus_tag="ACHK74_004779"
     CDS             complement(181..1374)
                     /gene="tyrB"
                     /locus_tag="ACHK74_004779"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFP0223465.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1628..2707)
                     /gene="alr"
                     /locus_tag="ACHK74_004780"
     CDS             complement(1628..2707)
                     /gene="alr"
                     /locus_tag="ACHK74_004780"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFP0223466.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2760..4175)
                     /gene="dnaB"
                     /locus_tag="ACHK74_004781"
     CDS             complement(2760..4175)
                     /gene="dnaB"
                     /locus_tag="ACHK74_004781"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP0223467.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4258..5241
                     /gene="qorA"
                     /locus_tag="ACHK74_004782"
     CDS             4258..5241
                     /gene="qorA"
                     /locus_tag="ACHK74_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFP0223468.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5407..5649)
                     /gene="pspG"
                     /locus_tag="ACHK74_004783"
     CDS             complement(5407..5649)
                     /gene="pspG"
                     /locus_tag="ACHK74_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFP0223469.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(5783..6820)
                     /gene="dusA"
                     /locus_tag="ACHK74_004784"
     CDS             complement(5783..6820)
                     /gene="dusA"
                     /locus_tag="ACHK74_004784"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFP0223470.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(7183..7890)
                     /gene="yjbM"
                     /locus_tag="ACHK74_004785"
     CDS             complement(7183..7890)
                     /gene="yjbM"
                     /locus_tag="ACHK74_004785"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFP0223471.1"
                     /translation="MWVNKYIDDCTDEDLNDRNFIASVVDRAIFHFAINSICNPGDNK
                     DATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(7914..>7991)
                     /gene="yjbL"
                     /locus_tag="ACHK74_004786"
     CDS             complement(7914..>7991)
                     /gene="yjbL"
                     /locus_tag="ACHK74_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFP0223472.1"
                     /translation="IIAKIDLFIQRITHNLWHARKKMCF"
BASE COUNT         2082 a         2063 c         1887 g         1959 t
ORIGIN      
        1 aaaaaaacag gataaggaat tccagataat taggataagc ttaacaataa aagaactaat
       61 aacgtttatt atctctcacg tagaacgatg gcatcaaaaa aatcatctat aaagaaagag
      121 tgaaagcgac gacagtttaa tttcactgca ggctgggtag ctccagcctg ctttcctgca
      181 ttacatcacc gcagcaaacg cctttgccac acgttgtaca tttgccgtat ttaacccggc
      241 gacacacatg cgaccgctgg cgatgagata gacaccaaat tcttcacgta gtcggtcaac
      301 ctgagcggca cttaaaccgg tataactgaa catgccgcgc tgattaagca gataatcgaa
      361 attgcgttct ggcatctctg tgcttaatac cttcaccaat tcctgacgca ttgccaaaat
      421 gcgagtacgc atctcttcta cttccgccag ccagctggct ttcaatgcct cgtcattcag
      481 cactgcagcc accacctgcg caccaaaatt cggcgggctg gagtagttgc ggcgaactgt
      541 tgctttcaat tgccccagta cgcggccagc ggcttcggca tcttcacaca gaacagaaag
      601 tccgccgacg cgctcgccgt aaagggagaa aattttcgag aacgaattgc tcaccagagc
      661 gggtaatcca gcgctggcaa tggcgcgaat agcgtaggca tcctcttcca taccggcacc
      721 aaatccttga taggcaatat cgaggaatgg aataagctcg cgggctttga gaatttcaat
      781 caccgcatcc cactgatcat tagtgagatc ggcacccgtt gggttgtggc aacatggatg
      841 cagcaacaca atactgcggg caggtaatgt tttcagcgtc gccaacaggt cattaaagcg
      901 cacgccgtta gtcgcttcgt cataccaggg gtaagtactc acttcgaatc cagccccggc
      961 gaatattgct acgtggtttt cccaggtagg atcgctgacc cagacgcctg attccgggaa
     1021 gtagcgtttc aggaaatccg cgcccacttt caatgcccct gagccgccaa gggtttgaat
     1081 ggttgctacg cgctgttgtt gcagtactgg atggtccgca ccaaacagca gcggcgcaat
     1141 ggcatggcga tagctgttaa gcccttccat cggtaaataa agcgaagcgc catgaggctg
     1201 cgcattcagg cgcgcttccg cctccgccac ggcttgcagt tgtggaataa ttccgtcttc
     1261 gttgtagtac agaccgatac ttaaattcac tttgtcgctg cgagggtctt ctttaaaacg
     1321 ctccataagc gtaagaatcg ggtcgccagc gtaggcgtca actttttgaa acacgcgatg
     1381 gttctccagg tttacgggca ggtggttaaa acacaataaa ccggaagaag gcgaagatcg
     1441 agtggatgtt caggagcgaa cggcaattag caacagagtg agactcatga caaacgtaca
     1501 tccgccagag gcacgacctt tataagagca tgaaagaaca tcaacttatt gaatttttta
     1561 ggattttatt ggccggataa ggcattcacg ccgcatccgg cacagacaat caaatattac
     1621 agaacgatta atccacgtat ttcatcgcga cccttgaagt caggcgcgta ataagttcgt
     1681 aagcgcttac tttcgtcatt tcagcgatac gttctacggg caaaccttcg ccccataaaa
     1741 tgaccggatc cccggctttg tcctgcgctt gtggacctaa gtctacgcag atcatatcca
     1801 tcgccacgcg cccgacaatc ggtacttcgc gaccgttcac cagcactggc gtaccggacg
     1861 gcgcggcgcg cggataacca tcgccatagc ccatcgcgac tacgccaaga cgggtatcac
     1921 gttcgcttac ccaggttcca ccataaccaa caggttctcc ggctttatgc tcacgcacgg
     1981 caatcaggct ggaggttagt gacatcactg gctgacagcc aaaatcggca ccggtggagc
     2041 gatcttccag cggcgagacg ccataaagaa tgatgcccgg gcgcacccag tcaaaatgcg
     2101 actgtggcca cagcagaatg ccacccgatg cggcaatgga acgttgacca ggtttgcctt
     2161 cgcaaaaggt attaaagata gcgagttgtt tctcggttgc gccacatttt ggttcatccg
     2221 cgcgcgcaaa atggctgacg atattcaccg gctgacgaac gtttttgcac tgggtcaggc
     2281 gatgataaaa cgcctcagcc tgttccggcc ttacgcccag acggtgcata ccggtatcga
     2341 gtttcatcca gacggtaacc ggctcgtcca ggctagcctc ttccagcgca gccagctgtt
     2401 cttcgttatg cacggcggta tgaaaatgtt gcgcagaaat cgtcggcaga tctctggcat
     2461 caaaaaagcc ttcgagtaac agtacaggtt tggtgattcc ccccgcacgc agtcgcagag
     2521 cttcttcgag acgggctacg ccaaaggcgt cagcatcggg gagcgttcgc gcggtctcaa
     2581 gaagaccgtg accataagcg ttcgctttca ccaccgcaac cattttactg gcaggggcca
     2641 gttcacgaag acgttgcagg ttgtgtcgca gagcgcggcg gttaatcaca acagttgccg
     2701 cttgcatttg tgttccttga taagtgtttg ctttaattac ctaattcata aaataattat
     2761 tattcgtcgt cgtactgcgg ccccgcatag ttgtcgaagc gcgaccattg accgttaaag
     2821 gtcaggcgta ccgtcccgat tgggccgtta cgttgtttac cgataataat ttccgcgatg
     2881 ccttttaaat cactgttttc gtgatacacc tcatcacgat agataaacat gatcaagtcc
     2941 gcatcctgct cgatagagcc agattcacgc aggtcggagt tgaccgggcg tttgtcggca
     3001 cgttgttcca gagaacggtt caactgggac agcgccacca ccggcacgtt cagttctttc
     3061 gccagtgctt tcagcgagcg ggagatttct gcaatttcca gcgtacggtt atcggaaagc
     3121 gccggtacgc gcatcagttg caggtagtcg atcatgataa gcccgatgcc gccgtgttca
     3181 cgggcaatac ggcgtgcgcg ggaacgcact tccgttggcg tcaggccgga ggagtcatcg
     3241 atatagatat tgcgtttttc gagcaaaata cccatggtgc cggaaatgcg cgcccagtct
     3301 tcatcatcga gctgcccggt acggatttta gtctggtcaa cgcgcgacag cgacgccaga
     3361 gaacgcatca taatctgttc tgaaggcatc tccagcgaga agataagtac cggtttatcc
     3421 tgcaacatcg ccgcgttttc gacgaggttc atcgcaaatg ttgttttacc catcgacgga
     3481 cgcgcggcga cgatgatcaa atccgacggc tgcaagccag cggttttttt gttgagatcg
     3541 tcataaccgg tgtttactcc ggtaacgcca tcgtgtggct gctgaaacaa ctgctcaata
     3601 cgcgccacgg ttgcgtcgag cacatcggcg atgttcttcg gcccttcgtc tttgtttgca
     3661 cgactttcag caattttaaa gacgcgggat tcagcaaggt ccagcagatc ttcgctggtg
     3721 cgcccctgcg gatcaaaacc ggcttcagca atctcattcg caaccgaaat catctcacgg
     3781 acaacggcac gttcacgcac gatgtccgca taggcactga tgttcgccgc acttggcgta
     3841 ttttttgaca gctctgccag ataagcaaaa ccaccgacgc tatcgagttg cccctggcgt
     3901 tccagcgatt ccgcaagggt aatcagatcg ataggactac cgctttcctg caaacgcgcc
     3961 atttcagtaa agatatggcg gtgtgggcgg gtgtaaaaat cgtctgccac cacacgctcg
     4021 gctacatcgt cccagcgttc gttatccagc attaaaccgc ccaacaccga ctgctccgct
     4081 tcgatcgagt gcggaggcac tttcagcccg gcaacttgtg gatcgcgttc gcggggttca
     4141 gcctgctgtt tgttgaaggg tttatttcct gccatagtga atggagttac cgagataaag
     4201 aatgggtcga aactttacca tatgaagcag accctgacga tacgttctgg aggacacatg
     4261 gcaacacgaa ttgaatttca caagcacggt ggcccggaag tacttcaagc cgtagagttc
     4321 actcctgccg atccggcaga gaatgaaatc caggtcgaaa ataaagccat cggcatcaat
     4381 tttatcgaca cgtatatccg cagcggcctt tacccgccgc catcactacc cagcggatta
     4441 ggtaccgaag cggcaggcat cgtgagtaaa gtcggcagtg gtgtaaagca tattaaggca
     4501 ggcgatcgtg ttgtctatgc gcagtcggcg ttaggcgctt acagctctgt gcataacatt
     4561 aatgcggata aagcggcgat tctgcctgcg gcaatttctt ttgaacaagc tgcagcatct
     4621 ttcctgaaag gcttaacggt ttattatctg ctgcgcaaaa cctatgaaat taaacccgat
     4681 gaacagttcc tgttccacgc agcggctggc ggcgttggct taattgcctg ccagtgggca
     4741 aaagccctgg gcgcgaaact tatcggcacc gtaggaaccg cgcaaaaagc gcagagcgcg
     4801 ctaaaagccg gcgcgtggca ggttattaac tatcgtgaag agaatctggt cgagcgattg
     4861 aaagagatca ccggcggtaa gaaagtgcgc gtagtgtacg attccgtggg cagagacacc
     4921 tgggaacggt cgctggattg cctgcaacgc cgcggcttaa tggtcagttt tggcaactca
     4981 tcaggtgcgg ttaccggtgt gaacttaggc attctcaatc aaaaaggctc gttgtatgtg
     5041 acacgccctt ccctgcaagg ctatatcacc acgcgggagg aattaaccga ggccagtaat
     5101 gaactgttct ctttgattgc cagcggtgtg attaaggtcg atgtcgccga gcagcagaaa
     5161 tatccgctga aggatgcgca gcgtgcgcat gagattctgg aaagccgggc gacgcaaggt
     5221 tccagcctgc tgattccata aaagaaatag ggcttccacc tgggaagccc tttcttttta
     5281 tagttcggct gtatgtaggg tacagcacga tgaatctgtt agaggcgcaa tagtgacaga
     5341 tttgattatc aattcctatt ttgttctaag gataaaacct taggttgtga tcatccgcac
     5401 aatcccttag taacgccagc ggtcataacg ctgatatttc ggcatttttg gtgctttaat
     5461 cgccttaata acccacacca ccgcaatcgc cagtagtaac cacggcagca acttaatcat
     5521 caatgccagc ataccgccga ggaacataat ggccgtcgcc acaaccagcg cggcgataat
     5581 gcccagcaac gaaacgccgg tgaccatcag catgacaaaa aagccaatca caaaaagtag
     5641 ttccagcatg atgctctccc aaatatgaaa tctcttgctg gcattacaag aatcatgcca
     5701 aaaataatct attgatttaa cagcaaaacg ccccgcgacg gtgcgcaggg cgtggtgaat
     5761 ttgattactt tttggtgaaa agttaacgct tatccgccac cagtttgagc gcgtgttcca
     5821 gcacgttaat gtcagcaccc gctttatggg cattttcact taaataacgc cgccactgcc
     5881 gcgcgccagg aataccctgg aacaaaccca gcatatgccg ggtaatatgg ccgagatacg
     5941 tcccctggct gagttcacgc tcaatgtacg gatacatggc gcgcactacc gccaccggat
     6001 cggcatcggt atccgaggaa ccaaagatct cccggtctac cgccgccaga atacccggat
     6061 tctgatacgc ctcgcgcccg accatcacgc catccatatg ttgcaggtgt gctttggcct
     6121 cttccagcga cttgatacca ccgttaatcg acattgtcag atgcggaaag tcacgcttca
     6181 gttgatacac acgcggataa tcgagcggcg ggatttcacg gttttctttc gggcttaacc
     6241 ccgaaagcca ggctttacgt gcgtggatga taaacatctc acactcgcct ttgccggaaa
     6301 cggtgttgat gaaatcgcag agaaattcat agctgtcctg gtcatcgatg ccaatacgcg
     6361 ttttcaccgt cactggaatc gacaccacat cgcgcatcgc tttcacgcag tcggcaacca
     6421 gctgcgcatt acccatcaga cacgcaccaa acatgccgtt ctgcacccgg tcagacgggc
     6481 agccgacatt caggttgatc tcatcatatc cacgcgcttc tgccagcttc gcacactgtg
     6541 ccagcgccgc cggatcgcta ccgccgagtt gcaacgctac cggatgttct tcttcactgt
     6601 acgccagata atcaccttta ccgtgaataa tcgcccctgt ggtcaccatt tcggtataaa
     6661 gcaacgtatt gcgggaaagc agacgcaaga aatagcggca atgtctgtcc gtccagtcga
     6721 gcattggtgc aacgctaaac cgaccactcc agtgaacgtc agttttttca ggcattgcgc
     6781 tggtttggtt gattttttgc atttcagaat taccgtgcat tttcaaatgt agagattatt
     6841 ttatcaatat atcatggggt tatgttattc agcttcactg ttcaggaggc tcaatagcgg
     6901 ggtactatac cataacaaca ggaagcgcct gtctcattgc aaaagaaaat tgagatcctc
     6961 tcaaggcatg aagctctcac gaagtgatgg aaataatctt attagccgtt aactttgtta
     7021 aggccaatga taaacaatcc aggttcgacg ataaataaaa aatcacacat taaactctgg
     7081 tgatatatct ccctgctaat agcattgata gagaaaaaag aacccaataa atattgggtc
     7141 cttttatata atgccttcca tactatcgaa gaacttcaca tattatttct ccgatttaac
     7201 cccgaacaaa tcataaatta atttagaagt gtctgtaagt attttaatct cttcctttga
     7261 ggttgggtca aacgacttcg cgaagctaat taattgtggt gcagcatctc gcattttgct
     7321 taaaatatca ggatcgagcg ttccttcatt caccagatgt gacatcttat ccagatagcc
     7381 atcaaaattc atttctcccc catcagccag acgcttcatc tctcccaggt acttcttcat
     7441 atcacgttga gataattttt caaactgagc tttcaggtag ctctcattat tttcattaat
     7501 atacactgtt tctgataaat ctgcgtaggc actagaatat gaaagtgaaa ttaacagcga
     7561 agcaaataat tttaagctgt tgtcatcatg ttcctggcag gcattaacaa acgttaaaaa
     7621 accagaacct attgcttgaa aatgtatatt cgctaaaggt tcattatcct tagattcctc
     7681 ataaaatagc tgaaccgtgg agggaaggcc attcttagtt tctacatcaa aagtacattg
     7741 ttcaatggga gtcgcatctt tattatcccc aggattacat atactattaa tcgcgaaatg
     7801 aaaaatagcc cggtcaacaa ctgatgcaat aaagttacga tcgtttaaat cctcatcagt
     7861 gcaatcgtca atatacttat tgacccacat tttgagatca tttatttgct caattaaaaa
     7921 cacatttttt tacgtgcatg ccataaatta tgcgtgattc gttgaataaa caaatctatt
     7981 tttgcaataa t
//
LOCUS       DAZGSP010000167         7837 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZGSP010000167 DAZGSP010000000
VERSION     DAZGSP010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.168"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..255)
                     /locus_tag="ACHK74_004787"
     CDS             complement(<1..255)
                     /locus_tag="ACHK74_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0223473.1"
                     /translation="MNLIQQLAGIIEGNLNSPLSVNVLAERSGYSERWLYKLFQDVTG
                     ISVAQYIRRRKLTLAALLLRHTSRRITDISLMYEFSSPQAF"
     gene            406..1068
                     /locus_tag="ACHK74_004788"
     CDS             406..1068
                     /locus_tag="ACHK74_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase"
                     /protein_id="HFP0223474.1"
                     /translation="MSSRKFLTHHEVSLLLRAVLKGRHPERDACMILLAFIHGLRVSE
                     MLSLRLADLELESRKLCVRRLKNGFSTVHPLLTEEVQCIRNWLKARETIKLPAAEEER
                     DWLFISRSGRPLTRQRFYNLLAEAGRNAGLAVAVHPHMLRHGCGYALADNGIDTRLIQ
                     DYLGHRNIRHTVIYTASNSARFERLWKPSKRAKKQLFDPNCKLPVLMLQMLKKTGYAN
                     NN"
     gene            1435..1899
                     /locus_tag="ACHK74_004789"
     CDS             1435..1899
                     /locus_tag="ACHK74_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiF/GrlA family transcriptional regulator"
                     /protein_id="HFP0223475.1"
                     /translation="MPERRRRIKRHFFCPECVQDMQARPLYLIVTFWGMRTGRLLTVE
                     AVSREFHITQRTAKNILYYIEHEGRNHIRSRRFIQNEPTTGRRRALRILDIQDPQEIP
                     ASSGAELDVQLEPVAPAAKQSLRPLTKFQKLRQWMVSRKQNEQAPLELTEYG"
     gene            1896..2513
                     /locus_tag="ACHK74_004790"
     CDS             1896..2513
                     /locus_tag="ACHK74_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP0223476.1"
                     /translation="MKQIFLMSQCQYTRLGLFAVMESAGQHAVQVISVETPEQIVSHI
                     LSDGHLPGKQRVIVVDLTCHESSVTVRALWFLWNLSVLYSERHELRGTPCILFGHQEG
                     RNNIRYPFTWISPSQNFHQLKNIFLRILAVPSRYISSGYGFQRLSANERQVINALLGG
                     ETVSDIAMRMQTHYSNVCYYRQKAIFKIGLKNRNDIAWIMNRKFL"
     gene            2835..4055
                     /locus_tag="ACHK74_004791"
     CDS             2835..4055
                     /locus_tag="ACHK74_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter strand-loop-strand
                     O-heptosyltransferase"
                     /protein_id="HFP0223477.1"
                     /translation="MSTLKNTFFIPPPDTPTQAGPENIFYDFNDGARVLLPEGKWHVR
                     LLDADSENILFCCDVDKGWVTSSKKYFVRFRIQVFRQGEETPLLDQTLKLKDRPVLIS
                     FPTGTLGDLLGWFPYAERFQSLHKCRLECTMAQDIIDLLAPQYPQIQFSTPDKPRTVA
                     PYATYRVGLYFGGDTNNQPVDFRKVGFHRSAGYILGVDPREAPVRLDLSAPRVIQEPY
                     VCIATQSTCQAKYWNNGTGWSEVIAHLKSLGYRVLCIDRDAHYGQGFVWNHIPWGAED
                     FTGKLPLQERVNLLRHASFFIGLPSGLSWLAWATGIPVVLISGFSLPNSEFYTPWRIF
                     NSHGCYGCWDDTSLNFDHHDFLWCPRHKNTDRQFECTRLITGAQVNGVINKLHRSLTE
                     QGVEDTLEKGVSNE"
     gene            4048..7770
                     /gene="tibA"
                     /locus_tag="ACHK74_004792"
                     /gene_synonym="tib"
     CDS             4048..7770
                     /gene="tibA"
                     /locus_tag="ACHK74_004792"
                     /gene_synonym="tib"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003658957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/invasin glycoprotein
                     TibA"
                     /protein_id="HFP0223478.1"
                     /translation="MNKVYNTVWNESTGMWVVTSELTRKGGQRPRQIKRTVLAGLVAG
                     LLLPSQPVLAAAYDNQTIGSGGTSSSMSLGTGDTATNTTINDGGFQNVNSGGSATSTV
                     INSGGSQLVSSGGSATDTSINKDGLQIVSSGASATNTTIVDFGKQHVSGGSATDTTIN
                     RNGRQYISSGGSATKTTVNSGGRQHVYKNGNVTETIIMDGGRLQVEAGGSASKVTQNS
                     GGAIVTNTGADLSGTNAKGSFSIGGGSAVNMLLENDGYLTVYSGHQASDTTVGSSGTL
                     KVRSGGNAIETTIISGGMLQVEAGGSASKVTQNSGGAVVTNTSADLSGTNAKGNFSIA
                     GGSASNMLLENGGSLTVLNGHQATGTTINSGGDMMVEGIATDTTINSNGLLHVSSGGS
                     VTGTIVNNGGRENINSGGSATSTTLNSGGIQAVSSGGVATSTLVNSGGSQYVYSGGNA
                     LNASVNDGGRQFVSKGGIAIATSINSGGRQYVRIGGSATDTVINNTGRQFISSGGSTT
                     NTTINSGGGQYLYAGNATKTTVNNGGSQYVSSGGSATDTTVNSGGRQNVYIDGNVTET
                     TITSGGMLQVEAGGSASKVTQNSGGAVVTNTSAVVSGTNDNGSFSIAGGSAANMLLEN
                     GGYLTVFDGHQASDTMVGSDGTLDVRSGGVLYGTTTLTDKGTLVGDVVTNEGNLYYLN
                     NSAATFAGTLTGTGTLTQEGGGTRFSGLLSQDGGIFLKSGGAMTMDALQAKANVTTQS
                     GTTLTLDNGTILTGNVAGDNTGAGDMAVKGASVWHLDGDSTVGALTLDNGTVDFRPSV
                     TTRLTPAFQAVSLTLGSLSGSGTFRMNTDIASHTGDMLNVAGNASGNFVLDIKNTGLE
                     PVSAGAPLQVVQTGGGDAAFTLKGGKVDAGTWEYGLSKENTNWYLKVDTPPTNPDAGN
                     PGTGNPDAGKPDAGTSSSPVRRTTKSADAVLGMATAPAYVFNSELDNLRFRHGDVMQN
                     TRAPGGVWGRYTGSDNRISGGASSGYTLTQNGFETGADMVFDLSDSSLAVGTFFSYSD
                     NSIKHARGGKSNVDSTGGGLYATWFDNDGYYVDGVLKYNRFNNELRTWMSDGTAVKGD
                     YSQNGFGGSLEAGRTFSLNENAWAQPYVRTTAFRADEKDIRLNNGMKASIGATKSLQA
                     EAGVKLGMTLDVTGKEVKPYLSAAVSHEFSDNNKVRINDTYDFRNDISGTTGKYGLGV
                     NAQLTPNAGVWAEARYENGKQTESPITGSVGFRINF"
BASE COUNT         1997 a         1844 c         2121 g         1875 t
ORIGIN      
        1 aaaagcctgg ggcgaactga actcatacat tagtgatatg tctgttatcc ggcgactggt
       61 atggcgcaaa agtaaagcgg caagtgttag ttttctgcga cgtatgtact gcgctacact
      121 gatccctgtt acatcctgaa acagtttgta caaccagcgt tctgaatagc ctgaacgttc
      181 agcaagaacg tttactgaca gaggactgtt cagattccct tcgataattc ccgccagttg
      241 ctggattaaa ttcatttgat atacctgtgg gtccatgcca gttgactgtt cttaagagca
      301 atcggagttt tattctgctt tcctgacttt agcacgttta gccatagcct gatgggggca
      361 tcgtgtcact cttgaccgct ctgtttcatc tgaccggagc tattaatgtc ttcccgaaaa
      421 tttcttactc accatgaagt cagccttttg ttacgggcgg tgctgaaggg gcgccacccg
      481 gaacgtgatg cctgcatgat tcttcttgct tttatccacg gactcagggt cagcgaaatg
      541 ctttcgttac gtcttgctga ccttgagctc gagtcccgaa agctgtgcgt tcggcgcctg
      601 aaaaacggct tcagtaccgt gcatccctta ctgactgaag aagtacaatg tatcaggaac
      661 tggctcaaag cccgggaaac tataaaattg cctgcagcag aagaagagag ggattggcta
      721 tttatttcca gatcagggcg tccgcttacc cgtcaacgtt tttataactt gctggctgaa
      781 gcaggaagaa atgccgggct ggctgttgct gtccatccac atatgctgag acatgggtgt
      841 ggttacgcgt tggcagataa cggcatcgat acacgcctga ttcaggatta cctgggacac
      901 cggaatatcc gacatacggt gatttataca gcttcaaatt cggcccgctt tgaacggtta
      961 tggaagccgt caaaaagggc gaaaaagcaa ctttttgacc caaactgtaa actgcctgta
     1021 ttgatgttac aaatgttaaa aaaaacaggt tatgcaaaca acaattaatt aaaaatcgtt
     1081 tgatctaaat caataaaact cccgtatcaa atcccttctg taacaatatg gcattgatgt
     1141 ctgtcagttt tctgaatttt ccaaaactcc attaacagta tgaatggcca aaaacaccac
     1201 acacctaaga aaataaaacc atacaaaagc caaaatctag ttttttaatc tattgttgtc
     1261 aaaatgggtc attttgacaa caacagttcc tcttcttgtg cacctgtatt gcgcgttact
     1321 ctgcactgat aacccgacac atccggcaac agatgtttgt tgtaattact ggggggaggg
     1381 atgtcagtgc ttcttaattc atacttctga cggagagata ttcaggtggc gggtatgcct
     1441 gagagacgca gacgaataaa gagacatttt ttctgtcctg agtgtgttca ggacatgcag
     1501 gctcgaccac tgtatctcat cgtgaccttc tgggggatgc gaaccggcag gttactgaca
     1561 gttgaggcgg tcagccggga gtttcatata acccagagaa cagcaaaaaa tattctgtat
     1621 tacattgaac acgagggacg aaaccatatc cgcagcaggc gctttataca aaacgaaccg
     1681 acaactggac gaagacgcgc actgcggatc ctggatatac aggatccgca ggaaatcccg
     1741 gccagttcag gagctgaact ggatgttcag ttggaacctg ttgcccccgc agcaaagcag
     1801 tcactcaggc cactgacaaa atttcagaaa ctacgtcagt ggatggtatc gagaaaacag
     1861 aatgaacagg ctcctctgga gctgacagaa tacggatgaa acagatattt ttgatgagtc
     1921 agtgtcagta cacccgcctc gggctttttg cagtgatgga aagtgccggg caacatgctg
     1981 ttcaggttat ttccgtggaa acaccggagc agattgtgtc gcatattctg tctgacggac
     2041 acctcccggg gaaacagcgt gtcattgtgg tagatctgac ctgccatgag tcatcagtga
     2101 ctgtgagggc gctgtggttt ttgtggaatc tgtcagtcct ttattctgaa cgccacgaac
     2161 tgagagggac tccatgcatt ttatttggac atcaggaagg gcggaataat atcagatatc
     2221 cttttacctg gatttctccg tcacaaaact ttcatcaatt gaaaaatatt tttctgcgca
     2281 ttctggccgt cccttctcgc tatataagta gtgggtatgg atttcagcgt ttaagtgcta
     2341 atgagcgaca ggtgataaat gcacttcttg gcggggaaac tgtaagtgat attgcaatgc
     2401 gtatgcagac acattacagc aatgtgtgtt actaccgaca aaaggcaata tttaaaatcg
     2461 gtctgaaaaa ccggaatgat attgcatgga ttatgaacag gaagtttttg taagcactgt
     2521 aacggtacgt actgaccgga tacacttccg aaattgataa gtctttccgc acgtaaactg
     2581 taaactaact cctgcgcgta gccggaagtt atgcagagag catttcatcg gcaacgacta
     2641 aagtcgttat aactataatg gaatgcgcga tgtgtgacat cgtaagaatg taatatgtga
     2701 gctcctctct gctattacag aagaatatca gaaccgtgcg atgggtgtta ctcatcataa
     2761 gaaagatact tcagttatat ttttagcgta ctcatcgtgc ttttcaacgc ttatatattt
     2821 ttgaaggtga aaatatgtca acgctgaaga atacattttt tatccctcca cctgatacgc
     2881 cgacgcaggc agggcctgaa aatatttttt atgactttaa tgatggtgca cgtgtattgc
     2941 ttcctgaagg gaaatggcat gtgcgtctgc tggatgcgga ttcagaaaat attcttttct
     3001 gctgcgatgt ggataaaggc tgggtaacca gcagtaagaa atattttgtg cgctttcgta
     3061 ttcaggtatt ccgtcagggg gaagaaacgc ccctgctgga tcaaaccctg aaattaaaag
     3121 accggcctgt actaatttca ttcccgactg ggacgctggg cgatttgctc ggctggtttc
     3181 cttatgcgga acgcttccag tccctgcata agtgccgtct ggaatgcacg atggcgcagg
     3241 acattattga tctgctggcg cctcagtatc cgcagataca gttttccacg ccggataagc
     3301 cgcgcactgt agcgccttat gccacctatc gtgtggggct ctatttcggt ggcgatacaa
     3361 acaatcagcc tgtggatttc cgcaaggtcg gtttccaccg ctctgcgggc tacattctgg
     3421 gcgttgaccc gcgtgaagcc cccgtccggc ttgacctgtc tgccccccgg gttatccagg
     3481 agccttatgt gtgcatcgcc acgcagtcca cctgccaggc gaaatactgg aacaacggca
     3541 ccggctggag tgaggttatc gcacacctga aatcactggg ttaccgggtg ctgtgcattg
     3601 accgggatgc ccactatggc cagggctttg tctggaatca catcccgtgg ggtgcagagg
     3661 actttaccgg caagcttccg ctgcaggaga gggtgaacct actgcggcat gccagtttct
     3721 tcattggcct gccaagcggc ttgtcatggc tggcctgggc aaccggtatt ccggtggtgc
     3781 tgataagcgg cttcagcctg cctaacagcg agttttatac cccgtggcgg atattcaact
     3841 cccatggctg ttatgggtgc tgggacgata catcgctgaa ttttgaccac cacgattttc
     3901 tgtggtgccc gagacataaa aacactgacc ggcaatttga atgtactcgg ctgataactg
     3961 gcgctcaggt caatggcgtc atcaataaac ttcaccgttc tttaactgaa cagggtgttg
     4021 aggacactct ggagaaagga gtaagcaatg aataaggttt ataacactgt ctggaatgaa
     4081 tccaccggga tgtgggtcgt tacctcagaa ctgacccgta aaggaggtca gcgcccccgg
     4141 caaatcaagc gtaccgtgct ggcagggctg gttgctggtt tgcttttgcc gtcacagcct
     4201 gtactggcgg cagcctatga caatcagaca attggaagtg gtggaacaag ctcatccatg
     4261 tccctgggta cgggtgatac ggcgacaaat accaccatta acgatggagg gtttcagaat
     4321 gtcaacagcg gcggtagtgc cacgagtacc gtcattaata gtggtgggag ccaactagta
     4381 tccagtggcg gtagtgctac ggacaccagt attaataaag atgggcttca gattgtatcc
     4441 agcggagcaa gtgccacaaa tacaaccatt gttgacttcg ggaagcagca tgtcagcggc
     4501 ggaagtgcca cggacaccac tattaacagg aatggacgcc aatatatctc cagtggtggc
     4561 agtgccacta aaacaaccgt taacagtggc gggcgtcagc atgtttataa aaacggcaat
     4621 gtgacagaaa cgatcattat ggacggcggc aggctgcagg tggaagccgg tggctcagcc
     4681 tctaaggtta ctcagaacag tggcggtgct atcgttacta ataccggtgc agacctgagt
     4741 ggtaccaacg ctaaaggcag cttcagtata ggtggcggca gtgcagtcaa tatgctgctg
     4801 gagaacgatg gctacctgac ggtttacagt ggtcatcagg ccagcgatac aaccgttggc
     4861 agcagcggga cgctgaaagt ccgcagcggc ggaaatgcca tagaaacgac gattatcagc
     4921 ggcggcatgc tgcaggtgga agccggtggc tcagcctcta aggttactca gaacagtggc
     4981 ggtgctgtcg ttactaatac cagtgcagac ctgagtggta ccaacgctaa aggcaatttc
     5041 agcatcgctg gcggcagtgc ttccaatatg cttctggaga atggtggctc attaactgta
     5101 ctcaatgggc atcaggccac aggtaccact attaacagtg gtggagatat gatggtcgag
     5161 ggcatcgcta ctgacacaac cattaacagc aatggcttac tgcatgtatc cagcggcggt
     5221 agtgtcactg gcaccatcgt taataacggt ggccgcgaga atatcaattc aggcggcagt
     5281 gccacgagca ctacccttaa cagtggtggt atccaggctg tctccagcgg cggtgttgct
     5341 acgagcaccc tcgttaacag cggagggagc caatatgtct acagcggcgg caatgcgttg
     5401 aacgcttctg ttaatgacgg tgggcgtcag tttgtctcca aaggcggtat cgccattgct
     5461 acttctatta atagcggcgg tcgccagtat gtcagaatcg gcggcagtgc cacggacacc
     5521 gttattaaca atactgggcg ccagtttatc tccagcggcg gcagtaccac taacactaca
     5581 attaattccg gtggggggca gtatctctac gccggaaatg ccacgaaaac tactgttaac
     5641 aacggcggga gccagtacgt ctccagtgga ggcagcgcca ctgacacaac cgttaacagt
     5701 ggtggacgtc agaatgttta catcgacgga aatgtcacag aaacgactat tacaagcggc
     5761 ggcatgctac aggtggaggc cggtggttca gcctctaaag ttacccagaa cagtggtggg
     5821 gctgtcgtca ccaataccag tgcagtcgtg agtggtacca acgataacgg cagcttcagt
     5881 atcgctggcg gcagtgcagc caatatgctg ctggaaaatg gtggatacct gacggtgttt
     5941 gacggtcatc aggccagcga tacgatggtt ggcagtgacg gtacgctgga tgttcgcagc
     6001 ggcggtgtgc tctacggcac cacaaccctg actgataaag ggacgttggt cggtgacgta
     6061 gtcacaaacg aaggcaacct gtattacctc aacaacagtg ctgcgacctt cgcaggcacc
     6121 ctgacgggga ccggcaccct gacgcaggaa ggcggtggca cccgcttcag cggcctgttg
     6181 tctcaggacg gtgggatttt ccttaagagt ggcggtgcca tgacgatgga tgcacttcag
     6241 gctaaggcta atgtgacgac acagtcaggc accaccctga cgctggataa cgggaccatc
     6301 ctgacgggga acgttgcggg agacaacacc ggtgcaggtg atatggcggt taaaggtgcg
     6361 tctgtctggc atctcgacgg cgactccact gtcggggcat taacgctgga taacgggact
     6421 gttgatttcc gtccgtcagt aaccactcgt ctgacgccag ccttccaggc ggtgtcgctg
     6481 acgctcggga gcctgtcggg tagcgggacg ttccggatga acacggatat cgcatcacat
     6541 accggagata tgctgaatgt cgcgggcaat gccagcggta actttgtgct ggatatcaaa
     6601 aacaccggtc tggagccagt atctgctggt gcgccgcttc aggtggtgca gacaggtggc
     6661 ggtgatgccg catttaccct gaaaggcggg aaagtcgatg ccggtacctg ggaatacggc
     6721 ctgagcaagg aaaataccaa ctggtatctg aaggttgata ctccgccgac taacccggat
     6781 gcaggtaacc caggtacagg taaccctgac gcaggtaagc cagatgcagg tacatcgtcg
     6841 tctccggtgc gtcgcacaac aaaatcggcc gacgcagtac tgggcatggc gacagcaccg
     6901 gcatacgtct tcaacagcga gctggataat ctgcgtttcc gtcatggtga tgtgatgcag
     6961 aatactcgtg caccaggggg cgtatggggc cgttataccg ggtcagacaa ccgcattagc
     7021 ggcggggcca gctcgggcta taccctgacc cagaacggtt ttgaaaccgg cgccgatatg
     7081 gtctttgacc tgagtgacag cagtttagcg gtaggtactt tcttctctta cagcgacaac
     7141 agcattaaac atgcccgtgg aggaaagagt aacgttgaca gcactggtgg tggtctgtac
     7201 gcgacctggt ttgataatga cggctactac gtggacggtg tgctgaaata caaccgcttt
     7261 aataatgagc tgcgtacctg gatgagtgac ggcacggcgg tgaagggcga ttacagccag
     7321 aacggtttcg gcggtagcct cgaagcgggc agaaccttca gtctgaatga gaatgcatgg
     7381 gcgcagccat acgtgcggac gacggcattc cgggcagatg agaaggatat ccgactgaat
     7441 aacggcatga aagccagtat cggtgcgacc aaatccttgc aggctgaggc tggcgtgaag
     7501 ctggggatga ccttggacgt tacaggaaaa gaggtcaaac cgtacctgtc ggcggcggtg
     7561 agtcatgaat tttcggacaa caacaaagtc cgtatcaatg acacctatga cttcaggaac
     7621 gatatctccg ggacgaccgg gaaatacggg ctgggtgtca atgctcaact gaccccaaat
     7681 gctggcgtct gggctgaagc tcgttatgaa aacggtaagc agaccgagtc accaataact
     7741 ggtagcgttg gtttccgaat caacttctga tgaatgggca gggatacgta ttagattttc
     7801 gatgtgaatc cctgcctgct ttcagtaaag attaatg
//
LOCUS       DAZGSP010000168         7739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZGSP010000168 DAZGSP010000000
VERSION     DAZGSP010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.169"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..215)
                     /locus_tag="ACHK74_004793"
     CDS             complement(<1..215)
                     /locus_tag="ACHK74_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP0223479.1"
                     /translation="MLKNLLNTEVVQVVEQAKDWREAVAISCRPLIENGSIEPRYVDA
                     IYRSHDTIGPYYVVGPGIAMPHARPEEG"
     gene            486..1517
                     /locus_tag="ACHK74_004794"
     CDS             486..1517
                     /locus_tag="ACHK74_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0223480.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSHTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKETEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVVGSPF
                     SPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQ
                     NTDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEITLGIMFECQRRLLKIPG
                     NIAVACLDGSDNCDQTHPTLTSIRIDYKKMGTETGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
     gene            1914..2183
                     /locus_tag="ACHK74_004795"
                     /pseudo
     CDS             1914..2183
                     /locus_tag="ACHK74_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167450.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2415..2513)
                     /locus_tag="ACHK74_004796"
                     /pseudo
     CDS             complement(2415..2513)
                     /locus_tag="ACHK74_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase IV"
     regulatory      2634..2822
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2887..4731
                     /gene="btuB"
                     /locus_tag="ACHK74_004797"
     CDS             2887..4731
                     /gene="btuB"
                     /locus_tag="ACHK74_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFP0223481.1"
                     /translation="MIKKYWFLMVYSVTAFSVWAQDTPLDTLVVTANRFQEPLSTVLA
                     PVTIVTREDIDRWQVSSVNDVLRRLPGVAISQHGGEGQLSTIFVRGTNSNHTLVLIDG
                     VRLNLAGVSGAADLSQFPVALVQRIEYIRGPRSAIYGSDAIGGVINIITSRENTGTEI
                     SVGWGSNSYQHYDISTHQQLGENTRVTLLGDYTYTRGFDAVAYGSTGMQPQSDRDGFL
                     SKTFYGKLEHNLSDTWSGFVRGYGYNNRTKYDAWYSPGSPLIDTRKLYSQSWDAGLRY
                     AGETLQSQLVSSYSHSKDYNYDPHYGRYDTSVNLDDMKQYNLQWTNSVTVGHGNVGAG
                     IDWQKQSTTPGTGYLPKGYDQRNTGIYLTGLQKLGDFTLEGAVRNDDNSQFERHTTWQ
                     SSAGWEFIEGYRFIASYGTAFKAPNLGQLYGMYGNPNLAPEKSKQWEGAFEGLTGGVN
                     WRISGYRNDISEMINYNPHTLRYYNDGKVHVKGIEATVNFDTGALTHTVSYDYTDARN
                     ALTDKPLERRPKLQVKYQLDWQVFDFDWGITYQYMGSRYDSDYSSWPYKSVKMGGVSL
                     WDVAVAYPVTPHLIVRGKIANLFNKDYETGYGYQAAGREYILSGSYTF"
     gene            4745..4936
                     /locus_tag="ACHK74_004798"
                     /pseudo
     CDS             4745..4936
                     /locus_tag="ACHK74_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5077..7164)
                     /gene="iha"
                     /locus_tag="ACHK74_004799"
     CDS             complement(5077..7164)
                     /gene="iha"
                     /locus_tag="ACHK74_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFP0223482.1"
                     /translation="MRITTLASVVIPCLGFSASSMAAEDVMIVSASGYEKKLTNAAAS
                     VSVISQEELQSSQYHDLAEALRAVEGVDVESGTGKTGGLEISIRGMPASYTLILIDGV
                     RQGGSGDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITKKNA
                     DKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQIRGSTQQRQGSSVTSLS
                     DTSATRIPYPTESQNYNLGARIDWKATEQDVLWFDMDTTRQRYDNQDGQLGSLTGGYD
                     HTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKSDKWGLAGKPREL
                     KESNLILNSLLLTPLGESHLLTVGGEYQSSSMKDGVVLASTGETFRQKSWSVFAEDEW
                     HLTDVLALTAGSRYEHHDQFGGHFSPRAYLVWDVTDAWTLKGGVTTGYKAPTMGQLHK
                     GISGVSGQGRTNLLGNPELKPEESVSYEAGVYYDNLSGLNANITGFITDFSNKIVSYS
                     IDDNTNSYTNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTQSEQRDGDNKGDPLSYT
                     PEHMVNAKLNWQITEEVASWLGARYRGKTPRFTRNYSSLSAVQKKVYDEKGEYLKAWT
                     VVDAGLSWKVTDALTLNAAVNNLLNKDYSDVSLYSADKSTLYAGDYFQTGSSTTGYVI
                     PERNYWMSLNYQF"
     gene            7103..7285
                     /locus_tag="ACHK74_004800"
     CDS             7103..7285
                     /locus_tag="ACHK74_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223483.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     IYLLQKMNITDKTKYK"
BASE COUNT         2091 a         1807 c         1792 g         2049 t
ORIGIN      
        1 ccctcttccg ggcgcgcatg aggcatcgca ataccagggc caaccacata gtacggaccg
       61 atagtgtcgt gcgagcgata gatcgcatca acataacgcg gttcgataga accgttttca
      121 atcaacggcc ggcatgaaat cgccacggct tcccgccagt ctttggcctg ttccacgacc
      181 tgaacgacct ctgtatttaa tagatttttt aacatgcgac tgataactcc cgatgataat
      241 taacaggaga gtagatattt tacgcattaa ttaatgtgat gattgtcgca tttgatagcg
      301 ctatctgata acgctaccaa tctttgtgat agcgctatca tttctctgtc gccatgcttt
      361 ggctatattc ggttcctgca tatacaatgc aacaggatat ttgctaccgt gggcaaaaac
      421 tggaaagagt aatggtaaaa taaaagtaat gaaaacagct tgttgtaatt atccatggag
      481 tcgaaatgga aaaaacacgt aagcgccgtg gtaccggacg ggtgacgctg caggaggtcg
      541 ctaactttgc aggtgtcgga accatgaccg tttcccgcgc gctgagaaca ccggaacagg
      601 tttctgacaa gctgagggaa aagatagaac aggccgtaga agagttgggt tatattccca
      661 acagaactgc tggcgccctt gcatctggtc acagtcatac agtcgccgta ctggttcctt
      721 ccctgacaga taaagccagc tcccgcttta tgcagtcgtt gcagcaggtg cttaataaaa
      781 atgaattcca gctactgctt gggtgtcatg agtataatca gctcaaagaa actgaaattc
      841 tgatgacgct tcttcaaggc aacccggcgg cgttagttat ttttggttct cagttggctg
      901 ataaaactca ccagatactc gagaaaacaa atatacctac cattaacgtt gtcggttccc
      961 ctttcagtcc ggctcaaatt accatcgaaa cagcattttt tgaagcatca cataaactga
     1021 ccgaacatct gctggagcag ggttataaga atatcggatt tatcggtgcg catatggaca
     1081 accgcctgca acgtcagcaa ctgaacggct ggcataaagc aatgctgagc cattaccaga
     1141 acactgatct tgttatcacc atgccggatg ccgcaagtct gcaattgggg cgttacgcat
     1201 taaacgagat attactgcga caaccagagc tggatgctgt gatttgcagc cacgaagaga
     1261 ttacgctggg tataatgttt gagtgccagc ggcgtttgtt aaaaatccct ggaaatatcg
     1321 ctgtagcttg tcttgacggt tcagacaatt gcgatcaaac acacccaact ttgacatcca
     1381 tccgtattga ctataaaaaa atggggacag agacgggtaa gctgctgatt ggactattga
     1441 ataataatca tgatgaatca gaagagtcgc gtatcgtgca attcaactac cagatagaat
     1501 tacgtcagag tacatgagcc taaaagagca ctattacaaa gttagctatt gtggtaggga
     1561 tagccgtttc cggctattcc ctgcgcagtt ccggatgtat gctgctaacg catccattcc
     1621 tacttcaggt atttggcgta gaagcggatg ctaaaaactt gccgcaaaac cgcaatatca
     1681 gcctgcagta tcgctctcca gattatccat tcctatattg atgacaacga caggtaagac
     1741 cgtgacctga taaacctgaa taagctcctg gacataaagt ccgtaatggg aataaggcga
     1801 gagtcagcga atctcatcga ggccagcagc actggctgca ataaggatat agagcagcaa
     1861 tctaccgtca acgtcaaaaa cacggaagcc ttgcaaacgg gatgcgttca tataaacaac
     1921 atggagcagc aacagtacga tgcctgttgt tgctcagtca ggaccttttc tgccatgcgc
     1981 gagccagcgt cacgattgat aaataatgtt cgcaactgat tactttactg caacagacgt
     2041 ggttggtaat aagcactggc ggacaatgca ggatggaagg ctgttttcag gttgtccgtt
     2101 caggtacgtt agatcagttc gttgcgctaa agacaactgt tcagtccctt atgttacccg
     2161 ttatggcaac cattgcccgg tgataaccgg gcgtgtcatc tgtgcatccg ttgattcgtg
     2221 tggactgata aagttttgtc tgctgaatct ttatacggaa tgctcttcag tatgcgtaag
     2281 ataatttgtt ttttggagga ttgtcgacaa tcagagggta aacaaaagaa tggttgaatg
     2341 tggatgaggt tcatattcat tgattcagat tttttgaaga atcaccacat gggatgtcca
     2401 tgtggttgta taactcaggg ccagcgctta ataatgatgg atgtattaat gccgccaaag
     2461 gcaaagttgt tactctgcaa aaacacacaa tcaaccttgc gggcttcatg caactaattg
     2521 agcatatatg ttttattctt attatggtcg ccgataaaat attatgtgta ttagatccga
     2581 gtgttcatgc attcctattt ttgagtcatg tatggacagt aacttgttac aaccagtaat
     2641 atccagttgt cggtcctgcg agttaatagg gaatccagtg caaatccgaa actgacacgc
     2701 agcggtaagg aaaggtgcga tgattgcgta atgtggacac ggtcatccgg tgggtggtca
     2761 tctctttgta tcttcagatg cccttctagg tctgaagacc tgtcggttaa cgtcgcatct
     2821 ggttctcatc atcgcttact atcgatggta actgcgatat tcttcttctg ttgtggatgc
     2881 gttacaatga ttaaaaaata ttggtttcta atggtgtact ccgtcacagc cttttctgtc
     2941 tgggcacagg atacccctct ggatactctc gttgttactg ctaaccgttt tcaggaacca
     3001 ttaagtaccg tgcttgctcc tgtaactatt gtgacacgag aagatataga tcgctggcaa
     3061 gtttcttctg taaatgatgt tctgcgtcgt ttgcctggcg ttgcaatttc tcagcatggg
     3121 ggagaagggc agctctcaac aatttttgtt cgtgggacaa actctaacca cactctggta
     3181 ttgattgatg gagtgcgcct gaatttagcg ggtgtcagtg gagctgcaga cctgagtcag
     3241 tttcccgtcg ctttagtgca gcgtatagaa tatatccgtg ggccacgttc agcaatttat
     3301 ggttcagatg ccattggcgg agtgataaat attattacat cccgtgagaa tactggcact
     3361 gaaatttccg taggatgggg aagtaatagt tatcaacatt acgatatatc cactcatcag
     3421 caacttgggg aaaatacccg tgtaacactt cttggcgatt atacctatac tagggggttt
     3481 gatgctgttg cctatggcag tacagggatg caaccacagt cagacagaga cggtttttta
     3541 agtaaaacat tctatggcaa acttgaacat aacttatctg atacgtggag cggatttgtt
     3601 cgtggctacg gctataataa ccgaactaaa tatgacgcct ggtactctcc cggttctcca
     3661 ctgattgata ctcgaaaact ttatagtcaa agctgggatg caggtcttcg ctacgctggt
     3721 gaaacactgc aatctcaact tgtttccagt tatagccata gtaaagatta caactacgat
     3781 ccacattatg gacgctatga cacatcagtt aacctggatg atatgaaaca atacaatctc
     3841 cagtggacaa atagcgtcac agtcggccac ggtaatgttg gcgctggtat cgactggcaa
     3901 aaacaatcca ctactcctgg aactggatat ctgccaaaag gttacgatca gcgtaacacg
     3961 gggatttatc tgacaggact gcaaaaactt ggcgatttta cgttagaagg cgcggtgcgt
     4021 aatgacgaca actcgcaatt tgaacgccac acaacatggc aatccagtgc gggatgggag
     4081 tttattgagg gttatcgctt tatcgcttcc tatggaactg ctttcaaagc gccaaatctg
     4141 gggcagctat atggtatgta tggtaatccg aatctggctc cggaaaagag taaacagtgg
     4201 gaaggggcat ttgaaggttt aactgggggc gtaaattggc gtatttcagg ataccgtaat
     4261 gatatcagtg aaatgattaa ctataatcct catacccttc gttattacaa cgacggaaaa
     4321 gtacacgtta aaggaattga ggctacagta aactttgata caggagcact gacgcatact
     4381 gttagttacg attatacgga tgccagaaat gccttaactg acaaaccatt ggaacgtcgt
     4441 ccaaaactgc aagtgaagta tcaacttgac tggcaagtat tcgattttga ctgggggata
     4501 acttatcaat atatgggatc gcgatacgat tcggactact catcctggcc ctataagtca
     4561 gtcaaaatgg gcggtgtgag tttatgggat gttgcggttg cgtatccggt cactcctcac
     4621 ctgatagttc gcggtaaaat agctaatctg ttcaacaaag attatgagac tggttatggc
     4681 tatcaggcag caggacggga atatatattg tctggcagct atactttctg aaatctgccc
     4741 caccgagtcg ctaaacagcg tgatccgcca tgcgatcaaa aagcgtaaag tgttcccgac
     4801 agacgaggcg gtgaaaaaag ttgtgtggct ggcaatccag tctgcgtccc agaaatggac
     4861 gatgccgttg aaggactggc gtatggaaat gagccgcttt attatcaagt tcagtgaccg
     4921 cctggacggt cacttctggg aaaaggcatt tacacagaat cctgaacagg ctcggtcact
     4981 tcgaccagac accgtgcgtc atgtaacgtt gccactgttc cgccaggagt tacagtggca
     5041 acgtatgcta ccgtcagtga tggcgttttg ttgttatcag aactgatagt tcagcgacat
     5101 ccagtaattt cgctcaggta tcacatatcc cgttgttgat gatcccgtct ggaagtaatc
     5161 accggcataa agcgtactct tatcggcact gtacaggctc acgtcactgt aatccttgtt
     5221 gagcaggtta ttcaccgcag cattcagcgt cagggcatcc gtcaccttcc acgacagacc
     5281 tgcatccacc accgtccagg ctttcagata ttctcctttc tcatcataca ctttcttctg
     5341 tacagcgctc agcgacgaat aattccgggt gaaacgtggt gttttcccgc gataacgggc
     5401 acccagccat gatgctacct cttcggtgat ctgccagttc agtttcgcat tcaccatgtg
     5461 ttcaggggta taactcagcg gatcaccttt gttatcacca tcacgttgtt cactttgggt
     5521 ccaggtgtaa ttcaacgaca gagtgacatc ctctgaccac agcggcaatg tgccggcgaa
     5581 ttccacacca tgcaagcggg cctttccgct gtttgtatag ctattggtgt tatcatcaat
     5641 ggaataagag acaatcttgt tggagaagtc tgttataaag cctgttatat tggcattcag
     5701 accggagagg ttatcgtaat atacccccgc ctcataactt acactctctt ccggcttcag
     5761 ttcggggtta ccgagtagat tagtacgtcc ctgcccggat acaccgctga tccccttatg
     5821 taactgtccc atggtggggg ctttatatcc cgtggtcaca ccgcctttca gcgtccaggc
     5881 atcggtcacg tcccagacca gatatgcacg cggactgaag tgtcccccga attgatcatg
     5941 atgttcatag cgactgcctg cagtcagcgc aagtacatcc gtgagatgcc actcatcctc
     6001 agcaaatact gaccagcttt tctgtcggaa agtttcacct gtgctggcaa ggacaacccc
     6061 atctttcatg gacgaactct gatactctcc ccccacagta agcagatgag attctcccag
     6121 aggggtaagc agcaatgaat tcaggataag gttagattcc ttaagctccc gcggtttacc
     6181 ggcaagcccc catttatcgc tcttcagaac actacgtaca agctcacgac ctttattttc
     6241 cgtctcgttc cagttcagat acgatttcca ggtaccgaag gtgaaagtat gatcatagcc
     6301 agccgaaatt ttgtttcgct catagcgcag agtatggtca tatccccctg tcaggctacc
     6361 cagttgacca tcctggttat cgtaacgctg tcgggtggta tccatatcaa accagagcac
     6421 atcctgctcc gtagccttcc agtcaatacg cgcaccaaga ttataattct gtgactccgt
     6481 gggataagga atacgcgtgg ctgatgtgtc gctcagtgat gtgaccgatg agccctgacg
     6541 ctgctgtgtg ctgccgcgga tctgcagact gacagaatca tccacaagag ggccactgct
     6601 ccagaaatta aactggctgc tgttgcccca tttgttactc tcctgcagat tcagcccggc
     6661 gttgacggaa gagagccatt tgtctgcatt ctttttggta atgatattca ccacaccacc
     6721 catcgcatca gagccataca gtgtggacat cggccccctg ataacctcaa tacgctcaat
     6781 cgcggccaga ggaggcatga accctgtatt catggcagaa aaaccgttag gcgtcacgtc
     6841 cccgctcccg ccctgacgga caccatcaat cagtatcagc gtgtaactgg cgggcattcc
     6901 acggatgctg atttccagcc caccggtttt acccgtacca ctttcaacat ccacaccctc
     6961 cacagctctc agtgcctccg ccagatcgtg gtactggctg gactgcaatt cctcctgact
     7021 gattacggaa acactggcgg ctgcgttggt cagttttttc tcatagccgg atgccgagac
     7081 aatcatcaca tcctctgctg ccatgctgct ggctgaaaac ccgagacagg gaatgacaac
     7141 ggaagccaga gtggttattc gcatattact aatctccgat catgttaatc ggcaagacaa
     7201 tacacaaata caaacaataa tgagaataat ttatatttac cttttacaaa aaatgaatat
     7261 tactgacaaa actaaatata aataatgact gcctcggcat gccagttaac agccatccgc
     7321 aatggcgtgt tcgattttgc gaataaaaaa tggttactgt agaagcggat tttcaccacc
     7381 tgtcaaccat gaatggtcag tggagtgaga cgactggtat gatttggtcg atgacttctt
     7441 ccggtgaata cggacagaaa cattgaaata ttgtaagaga gaacgttcag aattctgtgt
     7501 cacgtgaaaa tactacagcg ttatcacctt attcagtcga ccttcaaaat aaacgtctaa
     7561 ccgtgacaat caccatttgg attgcccact ggagaggcga acgattgcct cgacggcatt
     7621 tataatgcca gcccacattg ttggcgctga caatactaag gacaaacgat gagggagaac
     7681 cgtcttatac cactagcgct gacaccggca caccttcccc gtgaaataca ccggctgga
//
LOCUS       DAZGSP010000169         7688 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZGSP010000169 DAZGSP010000000
VERSION     DAZGSP010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.170"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            258..2495
                     /gene="nrfB"
                     /locus_tag="ACHK74_004801"
     CDS             258..2495
                     /gene="nrfB"
                     /locus_tag="ACHK74_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFP0223484.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2482..5454
                     /gene="nfrA"
                     /locus_tag="ACHK74_004802"
     CDS             2482..5454
                     /gene="nfrA"
                     /locus_tag="ACHK74_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFP0223485.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDASAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5455..6345
                     /locus_tag="ACHK74_004803"
     CDS             5455..6345
                     /locus_tag="ACHK74_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFP0223486.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6528..7289
                     /gene="envY"
                     /locus_tag="ACHK74_004804"
     CDS             6528..7289
                     /gene="envY"
                     /locus_tag="ACHK74_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFP0223487.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRVLLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
BASE COUNT         1912 a         2027 c         1959 g         1790 t
ORIGIN      
        1 ctgctcacct cctccaggca cttccactac atgatgacct gatcggtgcc accactcctc
       61 ttcactatga cgttaacctc ccctccttga caccacgctt ttgtctacat tctacgcaaa
      121 cggcaacttt tataaccagt gtaaaaataa cgtgccgcaa tagtcatacc tattaatggt
      181 aaaaagctgt cacaattcat aaaaaacctt aatatacgcc accctaaaca taaccagcgt
      241 taatgtaagg tttttgtgtg gactggcttc ttgatgtttt tgcaacctgg ctctacggct
      301 taaaagtaat cgcgataacg ttagcggtca tcatgttcat cagcgggctg gacgattttt
      361 ttattgatgt cgtctactgg gtacgccgca ttaaacgcaa gttgagtgtt tatcgccgct
      421 acccgcgaat gagttaccgc gaactgtata aaccagatga aaaaccgtta gcgattatgg
      481 ttccggcgtg gaatgaaacg ggcgtcatcg gcaatatggc cgagctggcg gcgaccacgc
      541 tcgactacga aaactatcat atctttgttg gcacctaccc caacgacccc gatactcaac
      601 gtgatgttga cgaagtgtgc gctcgctttc cgaacgtgca taaggtagtc tgcgcgcgtc
      661 ctggtcccac cagcaaagcc gactgtctga acaacgtgct ggacgccatc acccagtttg
      721 aacgtagcgc caatttcgct tttgctggtt ttattctgca tgacgccgaa gatgtgattt
      781 caccgatgga attgcgtctg ttcaactatc tggtcgagcg taaagatctg attcagatcc
      841 cggtgtatcc gttcgaacgc gaatggacgc acttcaccag catgacttac attgatgagt
      901 tttcagagct gcatggcaaa gatgttccgg tgcgtgaagc cctcgccgga caagtgccca
      961 gcgcaggcgt cggcacctgt ttcagccgcc gcgccgtgac cgctctgtta gctgacggtg
     1021 acggtattgc tttcgacgtg cagagtctga ctgaagatta cgacattggt ttccgcctga
     1081 aagagaaagg tatgacggaa atttttgtcc gttttccggt ggtggacgaa gccaaagaac
     1141 gcgagcagcg taaattttta cagcacgcac ggacgtcaaa catgatctgc gtgcgcgaat
     1201 atttccccga taccttttcg actgcagttc gacaaaaatc tcgctggatc atcggcattg
     1261 ttttccaggg ctttaaaacc cacaaatgga cctccagcct gacgctgaac tactttctct
     1321 ggcgcgaccg caaaggggca atcagtaact ttgtcagctt cctcgcgatg ctggtgatgc
     1381 tccagctttt gctgttgctg gcgtatgaaa gtttgtggcc cgatgcctgg catttccttt
     1441 ctatttttag cggcagcgca tggttaatga ccctgctgtg gctaaacttt ggcttgatgg
     1501 ttaaccgcat cgtgcagcgg gtgattttcg tcactggcta ctacggtctg acgcaggggc
     1561 tgttatctgt cctgcgtctt ttctggggca acctgattaa cttcatggcc aactggcgcg
     1621 cgctaaaaca ggtacttcaa cacggcgatc cacgtcgcgt cgcgtgggat aaaacaacgc
     1681 atgacttccc cagcgtgact ggcgataccc gctcgttgcg cccgttaggc caaattctgc
     1741 tggaaaatca ggtcatcact gaagaacaac tcgatacagc actgcgtaat cgcgtcgaag
     1801 gtctacgctt gggcggttca atgctgatgc aggggctgat tagcgccgag cagctggcac
     1861 aggcgctggc agagcaaaac ggcgtggcgt gggaatccat cgatgcctgg cagatcccgt
     1921 cctcgctgat tgccgaaatg ccggcctccg tagcgctgca ttatgcagta ctgccgctgc
     1981 gtctggaaaa tgatgagtta attgtcggca gtgaagatgg tattgacccg gtttcgctgg
     2041 cggccctgac gcgtaaagtc ggacgcaaag tgcgttacgt cattgttctg cggggacaaa
     2101 ttgtcacggg gttacgtcac tggtatgcac gccgacgcgg tcacgatccg cgggcaatgt
     2161 tgtacaatgc ggttcagcat cagtggctca cggaacagca ggccggtgaa atctggcggc
     2221 aatatgtgcc gcatcagttc ctattcgccg aaatactgac cacgctcggt catattaatc
     2281 gttcagcaat taacgtgttg ttattgcgcc atgaacgcag ttctctgccg ctcggcaagt
     2341 ttttggtcac cgaaggcgtt atcagccagg aaacgttgga tcgcgtcctg acaattcaac
     2401 gcgaattaca agtttcgatg caatcactat tactcaaagc aggtttaaac acagaacagg
     2461 ttgcgcaact ggagtccgaa aatgaaggag aataacctta atcgcgtcat cggatggtct
     2521 ggtttactgc tgacgtcttt attgagtacc agcgcactcg cagacaatat cggcaccagc
     2581 gcagaagagc tggggctgag cgattatcgc cattttgtta tttatccccg tctcgataag
     2641 gcgctgaagg cacagaaaaa taacgacgaa gcaaccgcca tccgcgaatt tgaatatata
     2701 caccagcagg tgccggataa tattccgctg actttatacc ttgcggaagc ctatcgccat
     2761 tttggtcatg atgaccgggc gcggctgttg cttgaggatc aactgaaacg tcacccagga
     2821 gatgcccgac ttgagcgcag tctggcggct attccggttg aagtgaaaag cgttacgact
     2881 gttgaagaac tgcttgccca gcaaaaagcg tgcgacgctg cgccgaccct gcgttgtcgc
     2941 agtgaagtcg ggcagaatgc cctgcggctg gcacaattac ctgtcgccag agcgcaactg
     3001 aacgatgcga cgtttgctgc atcgccagaa ggaaaaacgc tgcgaaccga tctgctgcaa
     3061 cgggcaatct acctgaaaca atggtcccag gcagatacgc tatacaatga agcacgccag
     3121 cagaacacat taagcgcggc agaacgccgt cagtggtttg acgtgcttct tgccgggcag
     3181 ctggacgatc ggatcctggc actgcaatca caggggatct tcaccgatcc tcagtcatat
     3241 attacttacg cgaccgcgct ggcttatcgt ggcgaaaaag cacgcctcca gcattatctc
     3301 aatgaaaata agccgctgtt taccacggac gcacaagaga aaagttggct ctatctgtta
     3361 tctaaataca gcgccaaccc cgttcaggca ttggcgaatt atacggtgca gtttgccgat
     3421 aaccgccagt atgttgttgg cgcgacgcta ccggtgctgt taaaagaagg tcagtacgac
     3481 gcagcgcaaa aactgctcgc caccctcccc gccaatgaaa tgcttgagga gcgttatgct
     3541 gtcagcgtgg cgacccgtaa caaggctgaa tctctgcgtc tggcacgatt gctgtatcag
     3601 caagaaccgg caaatcttac ccgcctggat caactgacct ggcaactgat gcagaacgag
     3661 cagtcacgcg aagctgccga tttattgctg caacgctatc ctttccaggg cgatgcgcgt
     3721 gtcagccaga ctttaatggc gcgactggcg tctctgctgg aaagtcatcc ttacctggca
     3781 acgccggcga aggtggcgat tttatcgaaa cccttaccgc tggcggagca acgtcagtgg
     3841 caaagtcagt tgccgggtat tgcagataat tgcccggcaa tagttcgctt gctgggcgat
     3901 atgtcgcctt cctacgatgc ttccgcctgg aaccgtctgg caaagtgtta tcgggacacg
     3961 ctacccggtg tggcgctgta tgcatggctt caggccgaac aacgacaacc gaacgcctgg
     4021 caacatcgtg cggtagccta tcaggcgtat caggttgagg actacgccac cgcactggcg
     4081 gcctggcaga aaatcagtct tcacgacatg agcaatgagg atctgcttgc tgctgccaat
     4141 accgcccagg cggcaggaaa tggtgcggct cgcgatcgct ggcttcagca ggcagaacaa
     4201 cgtgggctgg gaaacaatgc cctctactgg tggctgcatg cgcaacgtta cattcctggt
     4261 caaccggaac tcgcactgaa cgatctcacg cgctcaatca atattgcgcc ttctgccaac
     4321 gcttacgttg cgcgggcgac aatttatcgc caacgtcata atgtcccggc ggcggtgagt
     4381 gatttgcgcg ccgctctgga actggaaccg aataatagca acacccaggc agcgctcggt
     4441 tacgccttgt gggatagcgg tgatatcgca cagtcgcggg aaatgctcga acaggcgcat
     4501 aaagggctac cggacgatcc ggcacttatc cgacaactgg cctacgtgaa ccagcgtctg
     4561 gatgacatgc ctgcgacgca gcactacgcc cggctggtga ttgatgacat tgataatcag
     4621 gcgctgataa ccccactgac cccagagcaa aatcagcaac gcttcaattt ccgccgtttg
     4681 catgaggagg tcggtcgccg ctggacgttc agtttcgatt cttccatcgg cttgcgttcc
     4741 ggcgcaatga gtaccgctaa caataatgtc ggcggcgcag cgccagggaa aagctatcgt
     4801 agctacggac aactggaagc cgagtaccgc atcggacgca atatgctgct ggaaggcgac
     4861 ctgctctcag tttatagccg cgtctttgcc gataccggag aaaacggggt gatgatgccg
     4921 gtgaaaaatc cgatgtccgg caccggtctg cgctggaagc cgctgcgcga tcagatcttt
     4981 ttcctcgccg tcgaacagca gttgccgctg aacggccaaa atggcgcatc cgataccatg
     5041 ctgcgcgcca gcgcctcatt ctttaatggc ggcaaataca gcgacgaatg gcacccgaac
     5101 ggttcaggct ggtttgccca aaacctgtac ctcgatgcgg cgcaatatat ccgccaggat
     5161 attcaggcgt ggacggcaga ttatcgcgtc agctggcatc agaaggtagc taacggacag
     5221 actattgagc cttacgctca cgttcaggac aacggctatc gtgataaagg cactcagggc
     5281 gcgcagcttg gcggagtcgg agtccgctgg aatatctgga ccggcgagac gcactacgac
     5341 gcctggccgc acaaagtcag tctcggcgtc gagtatcaac atacctttaa ggcgattaat
     5401 caacgtaacg gagagcgcaa caacgcgttt ctcaccattg gagtgcactg gtaaatgcgt
     5461 aagttcattt tcgtattgct gacactgctt ttggtcagcc ctttttcctt tgcgatgaaa
     5521 ggtattatct ggcaaccaca aaaccgagat agtcaggtta ccgataccca gtggcagggg
     5581 ctgatgagtc agttacgttt gcaaggcttc gataccctgg ttttgcaatg gacccgttac
     5641 ggcgatgcat tcacccagcc agaacagcgc gcgttattgt ttaagcgtgc cgcagctgcg
     5701 caacaggctg gcctgaagct gattgtcggg ctgaacgccg atccggaatt ttttatgcac
     5761 cagaaacagt cgtccgcagc gctggaaagc tatcttaatc gcctgctggc tgccgatctc
     5821 cagcaagcca gattatggag cgccgcgcct ggcgtaacgc cggatggctg gtacatcagc
     5881 gcggaaattg acgacctgaa ctggcgcagc gaagctgccc gtcagccttt gctaacatgg
     5941 ttaaacaacg cgcagcggct gattagcgat gtttcagcaa aaccggttta tatcagtagt
     6001 tttttcgccg gaaacatgtc gcccgatggc tatcgccaac tgctggaaca agttaaagca
     6061 accggcgtta atgtctgggt acaggatggc agcggcgtgg ataaacttac cgctgaacag
     6121 cgtgaacgtt atctacaggc cagcgccgat tgccaaagtc ccgcccctgc cagcggcatt
     6181 gtttatgaac tgtttgtcgc cggcaaaggc aaaaccttta cagcgaaacc gaaaccggat
     6241 gcagagatcg cctcgctgtt agcgaaacgt tcctcttgcg gtaaagacac tctctatttc
     6301 tctctgcgct atttgcccgt cgcgcacggc attctcgagt attaaatctc ctccaggtaa
     6361 gtcgggtacg acctggctta cctctttcgc tctcgtcaga attccatcaa gattaattgc
     6421 taaaaagcgt gctaaagaaa gaagatcgtt atcggcgaac gggccgatca ctcatgcagg
     6481 aagcatagat cctttgagcg gagtttctga tatctgagga gtgcgaaatg caattgagca
     6541 gcagtgaacc ttgcgtggtg atcctgaccg aaaaagaggt agaggtaagc gtcaataacc
     6601 atgctacgtt tacccttccg aaaaactacc tggccgcctt cgcgtgcaac aataacgtca
     6661 ttgaactctc aacgttaaat cacgtattaa tcacccacat caaccgtaac atcatcaacg
     6721 attatctgtt gtttttaaat aagaacttaa cctgtgtaaa gccctggtcg cggctggcaa
     6781 ccccggttat tgcttgtcat agccgtacac cggaagtgtt ccggctagcc gccaaccaca
     6841 gcaagcagca acccagcaaa ccctgcgaag cggagttgac gcgcgtattg ctttttaccg
     6901 tattgtctaa ctttcttgag caatcgcggt ttattgccct actgatgtat atcttacgca
     6961 gcagcgtccg cgacagcgtc tgccgcatta ttcaaagcga tattcagcat tactggaatc
     7021 tgcgaattgt cgccagttcg ctatgtttaa gccccagcct gctcaaaaag aaattaaaaa
     7081 acgaaaatac cagctatagc cagattgtca cagagtgtcg tatgcgttac gccgtacaga
     7141 tgttattgat ggataacaaa aatatcactc aggtggcgca attatgtggc tatagcagca
     7201 cgtcatactt tatctctgtt tttaaggcgt tttacggcct gacaccgttg aattatctcg
     7261 ccaaacagcg acaaaaagtg atgtggtgaa gggcaaagcg gaaacggata agacgggcat
     7321 aaatgaagaa gaaatggcgc gccctgcagg attcgaacct gcggcccacg acttagaagt
     7381 tcctagaacg acattttaag tcaacaactt accgcgccat ctctgcgctc acacgtccca
     7441 ctacctcaaa acatgtaaag ccttgcaagc cattgtgagg ccttatgtgt ctcagttttg
     7501 tccctctttt ttgtactaaa aaacatagca attgaggata aacctcatgc tattttcgct
     7561 tatatgcctc taaaggcatg gcgcttaaat agacaaaaac accacaaaag cataaaaaaa
     7621 ccacacagta aaaccgcaat ataaaacaat aacagataat taaaccaaaa acagatagac
     7681 gcattgtg
//
LOCUS       DAZGSP010000170         7627 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZGSP010000170 DAZGSP010000000
VERSION     DAZGSP010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.171"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            25..552
                     /locus_tag="ACHK74_004805"
                     /pseudo
     CDS             25..552
                     /locus_tag="ACHK74_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            549..1535
                     /gene="ycdU"
                     /locus_tag="ACHK74_004806"
     CDS             549..1535
                     /gene="ycdU"
                     /locus_tag="ACHK74_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFP0223488.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(1728..1815)
                     /locus_tag="ACHK74_004807"
     tRNA            complement(1728..1815)
                     /locus_tag="ACHK74_004807"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1779..1781),aa:Ser,seq:gga)
     gene            2049..2987
                     /gene="ghrA"
                     /locus_tag="ACHK74_004808"
     CDS             2049..2987
                     /gene="ghrA"
                     /locus_tag="ACHK74_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFP0223489.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            3042..3779
                     /gene="ycdX"
                     /locus_tag="ACHK74_004809"
     CDS             3042..3779
                     /gene="ycdX"
                     /locus_tag="ACHK74_004809"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFP0223490.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3803..4357
                     /gene="ycdY"
                     /locus_tag="ACHK74_004810"
     CDS             3803..4357
                     /gene="ycdY"
                     /locus_tag="ACHK74_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFP0223491.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            4459..4950
                     /gene="ycdZ"
                     /locus_tag="ACHK74_004811"
     CDS             4459..4950
                     /gene="ycdZ"
                     /locus_tag="ACHK74_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFP0223492.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(5014..5847)
                     /gene="csgG"
                     /locus_tag="ACHK74_004812"
     CDS             complement(5014..5847)
                     /gene="csgG"
                     /locus_tag="ACHK74_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFP0223493.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(5874..6290)
                     /gene="csgF"
                     /locus_tag="ACHK74_004813"
     CDS             complement(5874..6290)
                     /gene="csgF"
                     /locus_tag="ACHK74_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFP0223494.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(6315..6704)
                     /gene="csgE"
                     /locus_tag="ACHK74_004814"
     CDS             complement(6315..6704)
                     /gene="csgE"
                     /locus_tag="ACHK74_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFP0223495.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(6709..7359)
                     /gene="csgD"
                     /locus_tag="ACHK74_004815"
     CDS             complement(6709..7359)
                     /gene="csgD"
                     /locus_tag="ACHK74_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFP0223496.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         1918 a         1676 c         1863 g         2170 t
ORIGIN      
        1 tccactattg ctgggtaaga tcaggcggtt aaaggcatca accgcgaaca actgcagacg
       61 atgattacgg aatcaggcat tgcgctggat attagctgtg ataacacgcc gcgccagcag
      121 gttattgggg gaacgcaggc tgcgttgaat gaatttgcca cgctgttgat ggccgctgga
      181 tacgaaccgg ttaaactcgg tgtgagcggt gcctggcaca cgcgtttgat ggaagacggc
      241 gttcaggcaa tgcgcgatta tcttgccgga ctggatatcg cttcgccaga gcatcaggtt
      301 ctgatgaacg taacagcaaa aagcgaggtc gctccgtcaa ttatcaaaga aaatctctca
      361 ttacacttaa ctcatacggt gaaatggact gaatctttcg atacatttct gaatatgcca
      421 accccggtag ctttccttga aataagtaat aagccctatt tggggaatat gttaaatgat
      481 tttgctggcg tggatcagca acgagttatg cattgtcgta aagcatttag tgatgcaaag
      541 gtattcaaat gattcctgat tatttaactt ttattcgctt tcaggataaa cgaaatctga
      601 tatacattta tgctattgga cttattctga taggctttta ttggaagaat gcagggttta
      661 cttttccatc agaggatatt ggtgtagtta gtgggattct ggccctggtg ctgtataatt
      721 ttatttttga tctcaaggcg tactgggctt ataaatgcgt cacgaagaat atcgattttt
      781 cgtggtttaa gaaaaagcag aaccacaaaa tagaattatt tcttacacaa cctctggtgg
      841 caggatttct gtcgttaatc atgttgagtg caatgagttg ggggctatac cagcttctac
      901 cctcgttata tgcgctgttc ctgatttcgt tacttgggcc gttggtcatc tttctgctgt
      961 ttcggatgat ccgcaccagt tatgtcaagc aggtcgctat ttcagtagcg aaaaaagtaa
     1021 aatataaaag tctgactcgc tatgtgctgc tttcggtgtg catctcaacg gttgttaacc
     1081 tgcttactat cagcccgttg cgtaacagtg attcttttgt gacagagggg cagtggttaa
     1141 cgtttaaatc gataattgca ttgctcattc tttgtggcgt agtgttggcg attaatctgt
     1201 tttttctgcg cttctccaag cggtacgctt ttctgggcag gctttttttg caggaaatcg
     1261 atctgttttt ctccagtgaa aatgcgttgt cgaccttttt tgccaagccg ctttggcttc
     1321 ggttattcat attgctggtt attgaaatga tgtggattac gctggtgtcg gtattggcaa
     1381 cgcttgtgga atggcggatt tggtttgaag cctatttttt actctgctat gtaccgtgct
     1441 taatttacta ttttttccat tgtcgattcc tctggcataa cgattttatg atggcatgtg
     1501 acatgtattt ccgttggggg cattttaata agtgaggaag ttacaggaag tgaccagata
     1561 acgcatatat gttctctact atcttgcgca ttttgattgt tgactgagta accagacagt
     1621 tgatgtgcac aatttcccct cgccctaaca gacgtgggcg ggggcacccg gtgtgtgcaa
     1681 tatacgaaaa aaaagcccgt actttcgtac gggctcttct ttaaatatgg cggtgagggg
     1741 gggattcgaa cccccgatac gttgccgtat acacactttc caggcgtgct ccttcagcca
     1801 ctcggacacc tcaccaaatt gtcgttcctg tcttgctgga acgggcgcta atttagggaa
     1861 atcatgacct gaggtcaaca aactttttga aaaaatcgcg cgtttattca aacttcaatc
     1921 aatgtgtggt tttagtaagc gaaatctgct ttttttgcca ccgaccacgg atttgttatg
     1981 ctggtggcct ttgtagatca taacgataag tgcgaataaa tttcgcacaa cgcttttcgg
     2041 gagtcagtat ggatatcatc ttttatcacc caacgttcga tacccaatgg tggattgagg
     2101 cactgcgcaa agctattcct caggcaagag tcagagcatg gaaaagcgga gataatgact
     2161 ctgctgatta tgctttagtc tggcatcctc ctgttgaaat gctggcaggg cgcgatctta
     2221 aagcggtgtt cgcactcggg gccggtgttg attctatttt gagcaagcta caggcacacc
     2281 ctgaaatgct gaacccttct gttccacttt ttcgcctgga agataccggt atgggcgagc
     2341 aaatgcagga atatgctgtc agtcaggtgc tgcattggtt tcgacgtttt gacgattatc
     2401 gcatccagca aaatagttcg cattggcaac cgctgcctga atatcatcgg gaagatttta
     2461 ccatcggcat tttgggcgca ggcgtactgg gcagtaaagt tgctcagagt ctgcaaacct
     2521 ggcgctttcc gctgcgttgc tggagtcgaa cccgtaaatc gtggcctggc gtgcaaagct
     2581 ttgccggacg ggaagaactg tctgcatttc tgagccaatg tcgggtattg attaatttgt
     2641 taccgaatac ccctgaaacc gtcggcatta ttaatcaaca attactcgaa aaattaccgg
     2701 atggcgcgta tctcctcaac ctggcgcgtg gtgttcatgt tgtggaagat gacctgctcg
     2761 cggcgctgga tagcggcaaa gttaaaggcg caatgttgga tgtttttaat cgtgaaccct
     2821 taccgcctga aagtccgctc tggcaacatc cacgcgtgac gataacacca catgtcgccg
     2881 cgattacccg tcccgctgaa gctgtggagt acatttctcg caccattgcc cagctcgaaa
     2941 aaggggagag ggtctgcggg caagtcgacc gcgcacgcgg ctactaataa agcatcagga
     3001 ttcctgctat ccttggcggg aattgaatac aggagagagt tatgtatccc gtcgaccttc
     3061 atatgcatac cgttgccagc acacatgcat atagcacatt gagtgattac attgcccagg
     3121 ccaaacaaaa gggcattaaa ctttttgcga tcaccgatca tggcccggat atggaagatg
     3181 ctccgcatca ctggcacttc attaacatgc gtatctggcc gcgagtggtt gatggggtag
     3241 ggatcctgcg cggcatcgaa gctaacatta aaaatgttga tggtgaaatt gattgcagcg
     3301 gtaaaatgtt tgactcgctg gatctgatta ttgccggttt tcatgagccg gtttttgcac
     3361 cacatgacaa agcgaccaat acacaagcga tgatcgccac tatcgccagc ggcaatgtgc
     3421 atataataag ccatcccgga aatcccaaat atgaaataga tgtgaaagcc gttgctgaag
     3481 cagccgcgaa acatcaggtg gcgctggaaa tcaataattc ttcattttta cactcacgta
     3541 agggcagtga agacaactgt cgtgcggtag ccgcagcggt acgtgatgct ggaggttggg
     3601 tggcattagg ctcggattct cacaccgcgt ttaccatggg ggaatttgaa gagtgtctta
     3661 aaatcctcga cgcggtagat tttccgccag agcgcatttt gaatgtttcg ccgcgccgct
     3721 tactgaactt ccttgaatct cgcggtatgg caccgattgc ggaatttgca gacctttaat
     3781 tactagtaat ggaaatatgt taatgaacga gttttctatc ctctgtcgtg tgctgggttc
     3841 gctctattac cgccaaccgc aagatccttt actggtgccg ctgtttaccc tgattcgtga
     3901 ggggaaactg gctgcgaact ggccactgga gcaggatgag ttactgacgc gtttacagaa
     3961 aagctgtgat atggcgcaag tctctgccga ttacaatgcg ttgtttatcg gcgatgaatg
     4021 tgccgtgccg ccatatcgta gcgcatgggt tgaggacgca acggaagcgg aagtgcgcgc
     4081 ttttctttcc gaacgaggga tgccactggc ggatacgcca gccgatcaca tcggcacatt
     4141 gctgcttgcg gcttcctggc tggaagatca gtcaacggaa gatgagagcg aagcactgga
     4201 aacactgttc agtgagtatc tgttaccctg gtgtggtgcg ttcctcggca aagtggaggc
     4261 ccatgcaacc acgcctttct ggcgcaccat ggcaccgcta acccgtgatg ccattagtgc
     4321 aatgtgggac gagctggaag aagattctga agagtaaatg tgattatcgt cactcataac
     4381 tgcaacagat aatttattgt tgcataaaat gtgtgctcga tctcattcat ggccgcgttt
     4441 tctgctatca tgcgcggcat gaacatactt ctctctattg caatcacaac aggcattctc
     4501 tccggtatct ggggatgggt ggctgtttcc ctgggcttac taagctgggc gggcttcctg
     4561 ggctgtacgg cctactttgc ctgcccgcaa ggtgggctga aagggctggc gatctccgct
     4621 gcaaccctgc ttagtggcgt ggtgtgggcg atggtcatta tttacggtag tgcactggca
     4681 ccacatctgg aaattctcgg ttatgtcata accggtattg tggcgtttct gatgtgtatt
     4741 caggccaaac agctgctgct ttcatttgtt ccggggacgt ttataggcgc atgcgcgacg
     4801 tttgccgggc agggtgactg gaaactggtg ttaccttcgc tggcgcttgg gctggtattt
     4861 ggttacgcaa tgaaaaacag cggcctgtgg ctggcggcgc gtagtgcaaa gacggctcac
     4921 cgtgagcagg aaatcaaaaa taaagcgtga ggggcactca cgctttcgct taaacagtaa
     4981 aatgccggat gataattccg gcttttttat ctgtcaggat tccggtggaa ccgacatatg
     5041 gcggtatttc accagaatgt cgttctgccg ttctgctttg ttctgcaaat cccacagacc
     5101 acggtcgata ccatcattaa tcaggaaaat gacccctgtt tcgatagccg acatcagaca
     5161 cagcataacg ggttcgtttg aggtgtaacc cacttcccct tccagcaagc gctggtagtc
     5221 aataaagcgg aaaaccccgg cctgaacttc ataggaaagt atcgtcttac tggtgttcac
     5281 cgaggagagg atctcaccgg tactcacatt gacgacgcgc aggttcacgg caatctgatc
     5341 gagctggtat tgcgtgtctg caccgatccc aaaatatctt gccccaacac cgcccgattt
     5401 aacgttgctt tcataaccga taatcgaacc ttcaaccata atatttgccg ccgttaaaga
     5461 ttgcagcggg attcggttat taatggcaac cgtgccgttt tcttgtgccg cacgaataat
     5521 cttgcgctcg ttaagcaggt tttgtaagcc ctggcgctcc agcggtataa accagcgaga
     5581 atctttcaat gccgtgacca gcattgccgt ggcgctttgc ggaacagcag tggagaagtt
     5641 acttgccggg tagggtttaa attgcccggt ttcgtcctga atgttgtata ccgaaacaaa
     5701 gatcttaccc gtcggcgctg gcagatgggt caaatctttg tagctctgag ctcgaggcat
     5761 taatgtcggt ctggcggctt ctttaggcgg ggcggttaag catccgctca gtaacatgac
     5821 ggcaaccaaa agaaataagc gctgcatgat tattttcctt atgaagctgg ggcttaaaaa
     5881 tcggttgagt tattttgtaa acccgaaacc tggatggtcg aggtttgtcc ggttttacga
     5941 tctgtcacgt tcaactgcaa ttgaccatcg cggttagcaa tatcaacaat ataatcgttg
     6001 gtcaccatgc ggcccggttt accggtatta atattcgaca gtagcccacc taaaatttgt
     6061 gactggatgg cctgagtaaa gttatctaac gctgagggtg tttcaatacc aaagtcatcg
     6121 ttatagctcg gatctttata agagttttgg gcctgagcgc tatttaataa aaaagcgcca
     6181 ttatttgggt taccaccaaa gtttggatta cggaactgga aagtcatggt tccagcccaa
     6241 cttaatggcg aaataagcat gagtagaact actgcatgtt tgacacgcat tgcagcctcc
     6301 gaacaatttt ttatttagaa ttcatcatgc gccaaatcgc ccgtacttaa taacgcctga
     6361 tttatctggc gacgatttag tgcttcttca gtttgaatca gtgcaaagac gacagttttc
     6421 tcgaagtctc ttttcaacgg aaataaaaaa gtctggaaaa taacgtcctg attgaccgtt
     6481 atagtgatcc agcttcccca tcgtgcactg ggcctttcat taatcgttaa gttacccgta
     6541 tagtcacttt cccatttatc actaaaggct cggtaaaaat catggccaat agatgaaaca
     6601 gtatggtcag ttaacaatcc cgggacttct acctcaacgg cgtgaagatt ccctgcggcg
     6661 aacagaaatt ctgctgccac aatccagcgt aaataacgtt tcatggcttt atcgcctgag
     6721 gttatcgttt gcccaggaaa ccgcttgtgt ccggtttttt acggctatct tcttgaaaag
     6781 attataaaga tgcgttttaa ccgtattttc gctgatgaac aacgaacgag cgatctcgtt
     6841 attagacgcg ccgatacgca gcttattcag gatctctttt tcccgatgag taaggagggc
     6901 tgattccgtg ctgttataac gatagttacc agaatgtgta atcaggtaac tggcaagctt
     6961 ttgcgtaaag tagcattcgc cgcgcaggac gccttgcaac ccattgacaa cacgttcttg
     7021 atcctccatg gcataaaaaa cgccgttgat atgaggccag ttttcaatgt cgcggtacgg
     7081 gtaatcttca ggcgtattta gcaacaatat tttgatattg ttgtttttcc tgctcaaagt
     7141 atcctgccaa taatggataa gctttttatc tgcttccatc atatccagaa gaataataga
     7201 gcctgaagat atatcgtcca gagaacgttg aatattatgt aattttcctg taattgccag
     7261 cgattgttta aggtgctgca agagagctgt cgcttgcaaa gacggtttag tgatcaacaa
     7321 taatgtatga ccatgaatac tatggacttc attaaacatg atgaaacccc gcttttttta
     7381 ttgatcgcac acctgacagc tgcctctaaa atagaagcac cagaagtact gacagatgtt
     7441 gcactgctgt gtgtagtaat aaatcagccc taaatgggta aaatataaaa ctaatggatt
     7501 acatctgatt tcaatctagc cattacaaat cttaaatcaa gtgttaaaca tgtaactaaa
     7561 tgtaactcgt tatattaaaa tgttaatctt aaggttttat taagtttaga aatgatagaa
     7621 aagttgt
//
LOCUS       DAZGSP010000171         7623 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZGSP010000171 DAZGSP010000000
VERSION     DAZGSP010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.172"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            213..857
                     /locus_tag="ACHK74_004816"
     CDS             213..857
                     /locus_tag="ACHK74_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP0223497.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            944..1252
                     /locus_tag="ACHK74_004817"
     CDS             944..1252
                     /locus_tag="ACHK74_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HFP0223498.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATVPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            1664..2644
                     /locus_tag="ACHK74_004818"
     CDS             1664..2644
                     /locus_tag="ACHK74_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFP0223499.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGGDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            2637..3053
                     /locus_tag="ACHK74_004819"
     CDS             2637..3053
                     /locus_tag="ACHK74_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFP0223500.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(3055..4329)
                     /gene="umuC"
                     /locus_tag="ACHK74_004820"
     CDS             complement(3055..4329)
                     /gene="umuC"
                     /locus_tag="ACHK74_004820"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HFP0223501.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMHLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVESYSIDE
                     MFIDLREVNRCISPEVFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLTQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPHKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(4329..4766)
                     /gene="umuD"
                     /locus_tag="ACHK74_004821"
     CDS             complement(4329..4766)
                     /gene="umuD"
                     /locus_tag="ACHK74_004821"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFP0223502.1"
                     /translation="MSTVYHRPAAPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(4763..5011)
                     /locus_tag="ACHK74_004822"
     CDS             complement(4763..5011)
                     /locus_tag="ACHK74_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP0223503.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            5295..5537
                     /locus_tag="ACHK74_004823"
     CDS             5295..5537
                     /locus_tag="ACHK74_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223504.1"
                     /translation="MNYDGDGRATARFFSGKGCRRAPLFTAPADVARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRE"
     gene            5635..6651
                     /locus_tag="ACHK74_004824"
     CDS             5635..6651
                     /locus_tag="ACHK74_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISShdy1 family transposase"
                     /protein_id="HFP0223505.1"
                     /translation="MTVVTVGIDLAKNVFAVHGVNAAGKAVLVKPLVRRAKLLELIAT
                     LPACLIGIEACTGAHYWAREFQKMGHTVRIMAPQLVSPYRMGGRHGKNDAADAAAICE
                     AVTRPNMRFVPIKSVEQQGQLFVHRAHQGYVSERTALINRIRGLLSELGIVLPQAFKA
                     FLSGVAQVLEDLPGYCNQVIGDLLNELYHIEEKIKNSEKLIQQLARDNSAAQLLMQFR
                     GIGPMTATAIVASIGNGHDFKNGRQFSAWLGLTPRQQSSGGKARLGRITKAGDSYLRT
                     LLVLGARSVLLGAAKNDDPVSRWALQLKERRGYGRALVAIAAKNARMCWAMLHLRDEF
                     IFPA"
     gene            7198..7272
                     /locus_tag="ACHK74_004825"
                     /pseudo
     CDS             7198..7272
                     /locus_tag="ACHK74_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7349..>7623
                     /locus_tag="ACHK74_004826"
     CDS             7349..>7623
                     /locus_tag="ACHK74_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFP0223506.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRFGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWHRIDRFMAAWKRAGFSVA"
BASE COUNT         1903 a         1703 c         1863 g         2154 t
ORIGIN      
        1 gatgcggtgt ctatgctcaa aagaattcct taagctctga cttttttaaa tccccttagg
       61 atatctcatg gatatattat cggtatttca tattctgttg acatgcagga ccggtataga
      121 gtaaaaaaaa ctctattgag tacctcttgg atatccgaaa ggtgtctttt gggttcttta
      181 tgggtattac atgtggattc tggagataca ctatgcctgt tattgctaat acacatccca
      241 aaggtggtgt gggtaagaca acttcatccg taaacattgt tggtgaaatg aagtctgata
      301 cagttgatct ggatactcat accgggcttt ctatcattct ggggctgaga cctgaaggaa
      361 aagaaatttc cgtgaaagta cccaaaacag tggatgagtt aatcgaaatt atgactccct
      421 acaagaacag cgataagacg ttacttattg actgtggggg atttgactca gatcttaccc
      481 gtactgcgat tgcctttgca gactgcgtta ttgtcccttc aaaagactcc ctgactgagc
      541 gtattggttt gatgcatttt gatggagtac tggacgaaat cagctctatc atggggaccg
      601 atataactgc tcacctgtat ctctgtaaag ttaatcctaa caagaagaaa ttccctaagc
      661 ttgatgcaat cctgccatcg ttcaaacatc tgaagctgat gaagagccgc atttctgctc
      721 gtgcagaatt tgaggatgtc attgagactg ggatgggaat cactgagtct gttcatgggc
      781 gctattcggc aggaggtaag gaagttattg cccttatcga agaaatcaat catctgattg
      841 aaaataacaa acagtaaagt gtatctattg ggtatccata tagtatcctt tgggctttta
      901 atggatatct tttgaatatc taacaaacat cctgggttat ctcatgagca aagcgaaatt
      961 tagtgcacag gctgaaaaac tgaaaaactt ggtggattct gccagattac aaacttcttc
     1021 ttctgccgta acagcaacag tcccgtctca gccaaatgaa ggtgcagagg aagtttcagg
     1081 tcgtcccgtg gccgccagga cgaaggctaa gaatcttaag gctataccgc tgtcgtattt
     1141 tgaagctcat gcggaactta aagcgaccag taaaacatct ctggatttta gctcatatat
     1201 tattgaggct ataagagaga agcttgaacg tgatggtgca ataggtcaat aaactatcag
     1261 ataccttaag ggtatctgat gtgtgtcttt taaatgcgtt atagggattc cttgtgtacc
     1321 taaagagtcc ctattagaca tctttctgta tatggtgaca tgattcgtca ctgctttcct
     1381 ttcctctcag tttcaccatt tcattccctt cccttaacct gaaccatgat ttcaaagatg
     1441 tttttatatt gcatttatgt gttgcatata acattcataa tgtttttgta tctatgattt
     1501 ctctggtctt aaggttgcat gtgcaatata catgcaattt attgtgtttt tatgttgctt
     1561 tcaccctttc agaaagacca tctttccatt aaaattgaat atacattgca tgtatgaagt
     1621 gtgtttgttt tttctatttg tttatgagca acggagactg gttatgaaaa tctttattga
     1681 tgatggttct accaacatta aacttgcctg gttggaggat ggtgacgtga aaacgttaat
     1741 cagccctaac agctttaaac cggaatggtc ttttagttta ctggatgatg ctgccccggc
     1801 taattacgag atcgacggcg agaagttttc atttgatcca ttaagtgctg atgctgtcgt
     1861 aacgactgaa acacgctatc agtacagtga tgtgaatgtt gtggccattc agcatgcgct
     1921 acagcagacc ggcctgaaag cgcagcctgt tgatgtcatc gtcacattgc cgatttctga
     1981 ataccttgac gccaacaatc agaaaaataa gcaaaacatt gaaagaaaga aaaaaaatgt
     2041 gatgcgtgaa gtacgggtgc agggggggga tgcatttgtt attcgcagtg tttccgttct
     2101 tcctgaaagt attcccgctg gtttcagtgt tcttgcgggg cttgaggatg atgaatccct
     2161 tttgatcgta gatctcggtg gtacaactct tgatgtgtcg catgttcgta gcaaaatgac
     2221 agggattaca aaaacctggt gtgatccgaa tatcggcgtg tccctgatta catccggggt
     2281 taaggaacag atggctgtac atgctaatac aagagtgagc tcattccagg ctgacaatat
     2341 cattgtgcac cgtaatgaac ctgactacct ttcccgtcgc atttataatg cggaacagcg
     2401 tgagtctatc attaatgtta tcaatgaacg gcagaagttg cttattaaac gagttaatga
     2461 tgtcatcagc cgtttcaccg actatacaca tgtaatgtgt gttggtggtg gggctgaaat
     2521 tgtggccgag gctgtgaaga acctgactaa ggttcctgat gagcgctttt atctgagctc
     2581 atccccgcag tttgatttag tcatgggaat gataaaaatg aaaggtggtg tgactaatga
     2641 gtgatgaaaa taaatccaga cgctgctcat ttgaactttt cccggatgaa cggacaggag
     2701 ataagatagc agatgagctc attgcaaatg aaaaactgaa ggagcgggga cgtttcatgc
     2761 gcgcgatgtt agttactggt gcggcgtttg cagctataga taaacgtctt cctttgctga
     2821 tatcagagtt actgacagaa aatacgactc tggatgatat taataaggtg atttccagcg
     2881 taataccagg tgctttttcg gttgaaaaga aattgcttga attactggaa aaacaatctg
     2941 gtctccatac gtctgtggat tgcagtactc ctcttacaga gcagagtctg tcgagaaatg
     3001 acggtgaaga ccagaccaga agaaacgctg aaaatatgtt cggagatgac tgaactaacc
     3061 cagcctggcc aggggaatat ctcgccattt agttgtataa cactgactca acatttcccg
     3121 tttcatttgc cattcagggg cggttcctcg ccccgcaaac cacactttcc cttttcccga
     3181 ctggttcagt tcatcgagtg ttttcattaa cttttcgctg tttttatggg gctggatctc
     3241 atcaaataat cccggctgcg ctatacccga tggtgtgaaa tcagccagca tgactcctgc
     3301 cttcatatag cggtaccctt cacgccagac atggtttaag gctctgcatg cggcggcaat
     3361 aatgtcccgg ctgtcctgtg tgggcaatgg aagcttttcc acagcggcat tgctgtaaca
     3421 gggttctttt actgcaaagg gggatgtccg tacaaatgtc gtcacctgcc ggcaatactg
     3481 acgctcccca cgtagtttct ctgcagcccg ctctgcatac tgaacaacag cctggtgcat
     3541 ggcatctttg tctgtgattc gttcaccaaa actgcgacta cagacaatct gctgttttgc
     3601 cggtggtgct tcttccaggg atatgcagga ctcgccgttg agttcgcgta ccgtacgctc
     3661 aagaatgacg ctgaagtttt tccggatgaa tgccgtgtta gcctgcgtca gctgcagtgc
     3721 tgtgttaata cccagcgcat tcagcttttc cgtcagtctg cgtcctactc cccagacttc
     3781 accaactggc tgcagcccca gtagcttcaa gatccgatta cggttttctg ccgtcagcgc
     3841 gaccacaccg gaaaactgtg gccattgctt tgttgcccac tgtgcacttt tagccagcgt
     3901 ttttgtaggt gcaatgccca cccccatggt gagtcctgtc cagctcttta cctgttccct
     3961 gagctgatga ccaaaaacct ccggagagat gcaacggttc acctcccgca aatcaatgaa
     4021 catttcatca attgagtagg actcaactgc gggagaaagc gactccagaa cagccataac
     4081 ccgttggctc atgctgtggt acagcgcata attgctggaa aatacatgta ttttcttctc
     4141 caggtgcatt tgtctgacct gaaaccaggg ctgccccatt ctgatgccaa gggcttttgc
     4201 ctccgggctg cgcgcgatca cacagccatc gttattgctg agtacgatga ccggttcgtt
     4261 acgaaggtcc gggcggaaaa ctttttcaca tgaggcgtag aaactgttga tatcagccag
     4321 tgcaaacatc agcgtaactc ccgcgtcctg tgtatcacgt gagtgacaac accaaaaata
     4381 cagatgtttt ccggatacag tgtgcggaac tccgggctgt ctgaaaccgg ctccagggcc
     4441 gggcgtgggc gcaacagcag tcgtttgacg gtgaactcac cgtcgatctc agcgataacg
     4501 atgtccccgt gttgtggttt ttcggccctg tccactacca gcagatcacc attctgcacg
     4561 ccagcctggt tcatcgattc accgctggcg cgcagaaaga aggtggctgc aggtctgctg
     4621 atgcaatagc tgttcagatc cagttcctgc tcagcataat cagtggcagg agaaggaaaa
     4681 ccggcctggc aacgatcggc aaacaacggt cgcacgtaac tgtcatcgcc tgaaggggca
     4741 gccggacggt gataaacggt actcatgctg caggcagcca gctgtcgtcc tgccagattt
     4801 cttccagcag agacaggata cgttctttat ccttgtcgtt gcgggctcct gtgacagata
     4861 cagacggggc gctgccctgg tctatacgca gccagatttc cgggtagtgt ggtttcagac
     4921 gttgttcgat ttcattctgt agcgcctgaa gtgtgcctga tttgaggttt tttgtgctct
     4981 ggcggtcgaa aagaatttca atgcggatca tgttctcatc tcccttttct tttgctgtat
     5041 gtatatacag cattctaata actgaatcca cacacagtca aatgtcatta ttgtgactaa
     5101 aaaacgctca attctctgag agtgtggcta tatgcctgct gtgggctgct gtgtccagct
     5161 tatccacagc attttgtgca cggttctgtg gacaaaatac ctggttaccc aggccgtgcc
     5221 ggcacgttaa ccgggctgca tccgatgcaa gtgtgtcgct gtcgccggcc tcctcacccg
     5281 gtcacttacg gtggatgaat tatgacggag acgggcgggc aaccgcccgt ttcttttccg
     5341 gtaaaggatg tcgccgtgct cctcttttta ctgcgccggc tgacgtggcg cggcataaac
     5401 gctgcctgtg gtcagtgtcc cgcgtccggc gggcacggga cgggcgcttt taccgctccc
     5461 ggctggcgtc ggtgacagtg tacgccagcc cgtcgccctt ttctgatgaa cgcccttcaa
     5521 gccgctttcg cgaatgaaag ggacatctta ttcggtcttt acgctgggga gttactttgg
     5581 aagactgcgt caacacgatg ataagcgtac aaagccattg ttaagggatc ctctatgact
     5641 gttgttactg tcggtattga tttagctaaa aatgtgttcg ctgtgcatgg cgttaatgct
     5701 gcaggaaaag ctgttttagt taagcctctt gtgcgtcggg ctaaattgct tgaattgatt
     5761 gcaacacttc cagcatgtct gattggaatt gaagcatgta caggtgcaca ttactgggca
     5821 agagagttcc agaaaatggg gcatacagtt cgaattatgg cgccgcaact ggtttctcca
     5881 taccgtatgg gtggccgtca tgggaaaaat gatgctgcag atgccgctgc catttgtgaa
     5941 gctgttaccc gtcctaatat gcggtttgtt cccataaaat cagttgagca gcaagggcaa
     6001 ttatttgtac acagagctca tcagggatat gtcagtgaga gaacagcatt gattaatcgc
     6061 atccggggat tgctcagtga gttaggtatt gttctcccac aggcgtttaa agcgttttta
     6121 tctggggtag ctcaggtcct tgaggatttg cccggttact gtaatcaggt tattggtgat
     6181 cttcttaatg agctttatca catagaagaa aagattaaaa acagtgaaaa actcattcag
     6241 caacttgcca gagataactc agcagcacaa ctactaatgc aattcagggg gataggcccg
     6301 atgactgcaa cagcaatcgt tgccagcata ggtaatggtc atgatttcaa gaacggacgt
     6361 cagtttagcg cctggctggg gctaacacca agacaacaaa gttccggggg gaaagcacgg
     6421 ttagggagaa taactaaagc cggcgacagc tatctcagaa cacttcttgt tttgggggcg
     6481 agatctgtac tgttgggagc agctaaaaat gatgatccgg ttagtcgatg ggccctgcaa
     6541 ttaaaagaaa ggcgaggata tggacgagcg cttgttgcga tagcagcaaa aaatgcacgt
     6601 atgtgctggg caatgttgca cctgagagat gaattcatat ttccagccta gtctactgat
     6661 tattaatgag tttatgaaat tgatttcctt ccttgcatga cgactgcgcg atgatgattt
     6721 aaaaggtttg gacctgcgcc atggtttact cgagatatgc aagaggctgc catgtttatg
     6781 tggatatctg tttcgcggat ggagtccatg gcgtgcgtct ttcgtcaggg ttcgttgcta
     6841 acaacagcac gttaataacc gtttataggc tcgctgtccg taacctttta ctctgactgt
     6901 gcaagttatg tgcagaaatc aatctcatga tagataaaag aattattcca ctgttgcacc
     6961 tggagtgttc ggtgctcgct ttgctgcgct ccgcctcaat ccagttgcaa cagcgtttaa
     7021 ttaaaagcat taaggcttgt gtcattgcgt acttgagcta tgcaggagat ttttattggt
     7081 tctctgtttc gcggatgaag tctctggcat gagcctttct ctcataaaaa tcattctatt
     7141 tcatatagat atattaattt catcatcatc gttgactcta caacagatgt ccttataggc
     7201 atagccttgc cgtcaaaaag acgacggctg cggtattcca cggtcggcct gacccgttac
     7261 cggacgcggt gaacagccca caggcagcgg ggaacgggca ccacaggggt gccctcctgg
     7321 tgccttctgt aagagaagga gtgttgttat gtcccgtttt gtcctcggta actgcatcga
     7381 tgttatggcc cgtattcctg ataacgccat tgatttcata ctgactgacc cgccgtacct
     7441 cgtcggtttt cgtgaccgtt tcgggcgcac catcgctggc gataaaaccg atgaatggct
     7501 gcaaccggcc tgtaatgaaa tgtaccgcgt actcaaaaaa gacgcgctga tggtcagctt
     7561 ctacggctgg caccgtattg accgctttat ggcggcctgg aaacgcgccg gattcagcgt
     7621 ggc
//
LOCUS       DAZGSP010000172         7540 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZGSP010000172 DAZGSP010000000
VERSION     DAZGSP010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.173"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            444..1682
                     /locus_tag="ACHK74_004827"
     CDS             444..1682
                     /locus_tag="ACHK74_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004172012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFP0223507.1"
                     /translation="MEVFMKDVSILNEMIFSASTENELQEKFKDIYHRINNESEYDDS
                     GSVEFLPLPRLNSRSERSEVQDIISVVIESGFFTSGPYIGKVEETLRLFYGAHTCIAT
                     SSGTDALKIALKSVGVEPGDEVIVPLNSFAATENAVMAVGAVPVFANIDSSFNMLPDE
                     VDRLRTSKTKAVLPVCLYGSCMYIDAIHRIARKADIPVIVDAAQCFGIRSLINHCDLL
                     ALSFNPFKNIGSLGKSGAVLTLSPELARLARQYSYHGFAEGKKNIKAQNWGLNSCMDN
                     LQAATLSVKLHHFENNAKKRCLLAARYHLLLSDLSHNIILPTETHQNTWHLFPILLCK
                     GERDSLFAFAREKGVELDIYYPVLSHCGEHPLATGYSRREQFSDSEKIHSALLHLPLH
                     NHMSLQEQNIVIEVLHDYFK"
     gene            1666..2496
                     /locus_tag="ACHK74_004828"
     CDS             1666..2496
                     /locus_tag="ACHK74_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HFP0223508.1"
                     /translation="MTILNKDWPVEFPFPTVIRTVVCSDLDETYIPSDSDKKKLSGIV
                     QLESYITSHAEKKGILAGWITGTNLDSVWRKSRGYISRSPHFICCSLGTEFYWVKNGR
                     LIPSETWAERISRSGYSRQNVNCLVKILMEKEIPLLKQPEDYQGPFKVSYYYPESPSI
                     ASDFATIQELADERRIRVVFTRCNPAAGDPSGCYDVEFIPLCCGKDQAVSFLMEETGL
                     PKEAIIAFGDSANDFAMFAQAGSGYLVGNADPFAVSQYGNRLNSAYCHGILSVLKELQ
                     "
     gene            2496..3512
                     /locus_tag="ACHK74_004829"
     CDS             2496..3512
                     /locus_tag="ACHK74_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP0223509.1"
                     /translation="MIIGIVGCGGIARAHVNALCLNPLVTGLALYDVNPDKVRQLSEI
                     SSLPVTNCQDLEQLAGVSQGFIICTPNHLHVSVAEEVLRYKQLPFVCEKPLSTDLNSA
                     RYLQEIAPKGSIVSFNYRYNPVIDMIMRLRNERKLGELHFFSAEFNKNSALTRHHLTW
                     RDSAQQSKSSGALGDLSCHLLDLFCFIGESPVVVHGIKTVKGTRVGTKSDGQVEVDDN
                     GYVMGSSEKGAYFRVHASKSETDQNLGLHIQLVFENGEIRYSTHHENRLLLILFNDTN
                     TETIGFDALKRLPDPPRELPFWSDSFIHLHDDWCALIKYGHSSPKLADLSSGLHIQEI
                     IEAF"
     gene            3514..3783
                     /locus_tag="ACHK74_004830"
     CDS             3514..3783
                     /locus_tag="ACHK74_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HFP0223510.1"
                     /translation="MDTILLTGLFAAFFTTFAFAPQSIKTIRTRNTEGISVVMYIMFL
                     TGVISWIAYGIMRSDFAVLIANIVTLFLAAPVLVITLINRRKKHV"
     gene            3780..5311
                     /locus_tag="ACHK74_004831"
                     /pseudo
     CDS             3780..5311
                     /locus_tag="ACHK74_004831"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
     gene            complement(5344..5796)
                     /locus_tag="ACHK74_004832"
     CDS             complement(5344..5796)
                     /locus_tag="ACHK74_004832"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFP0223511.1"
                     /translation="MKFMECAVRDVIYGTNVRIVKPVNIYECELRDNVFVGPFVEIQK
                     GCVIGSGSRIQSHTFICENVTLGENCFIGHNVTFANDLFRSGAPDPSPDNWISIILGD
                     SVTVGSGAIILSPYICSGAVIGAGAVVVKPVENKGIYAGNPARLIRIL"
     gene            6125..6382
                     /locus_tag="ACHK74_004833"
     CDS             6125..6382
                     /locus_tag="ACHK74_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223512.1"
                     /translation="MDEERVFSLSYEQLTRFAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPENDR"
     gene            6382..6972
                     /locus_tag="ACHK74_004834"
     CDS             6382..6972
                     /locus_tag="ACHK74_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223513.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLIPAAGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGIEVRVQINESAAP"
     gene            complement(6992..7339)
                     /locus_tag="ACHK74_004835"
     CDS             complement(6992..7339)
                     /locus_tag="ACHK74_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFP0223514.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLLPLPF
                     MPFWQVTVLMGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLCMASFHWW
                     RRKVNRLPPWDDV"
BASE COUNT         2026 a         1827 c         1773 g         1914 t
ORIGIN      
        1 gggttttcgg atctctggag cagaaaagaa aattattgcc agataatcac aaaaaatggt
       61 ggattattgt gatgaagtga ttattgcaat cagaacaata actggaattg taaagatcat
      121 attcatcaat aaatagcaga gtcacagatc tgctgaaagc caaaccatga agatactacc
      181 cgagtccgct tctggaagat ccatttcaaa tatccggata cacaggcaac tgtggcgacg
      241 attgtaatga cattaaccat atcggcttcc ctgaagcatt ggttatggca accacgttat
      301 caatatcttt ccatcatttt cagtaaatat cactaacaga ccgatattac cggacctgta
      361 agttatttgt gactgatatc accaatttgt aaatcaaatg tgaaaatatg gtttatctgt
      421 gctttatttt tccccgacat aaaatggagg tctttatgaa agatgtcagt attttgaatg
      481 aaatgatctt cagcgcatca acagaaaatg aactacagga aaaatttaaa gatatatatc
      541 accgtatcaa caacgagagt gaatatgatg attctggttc agtcgaattt ctgccacttc
      601 caagactgaa ttcccgttct gaacgctcag aagtacagga catcatttca gttgtaattg
      661 aaagcggatt ttttaccagt ggcccctata ttggaaaagt agaagagaca ttaagattat
      721 tttatggtgc tcatacctgt attgccacca gtagcggcac cgatgcgtta aaaatagctc
      781 ttaaatctgt tggagtggaa cccggagatg aggtcatcgt tccgctgaac tcctttgcag
      841 ctacggagaa tgcagtgatg gccgtgggag ccgttccggt cttcgctaat attgacagtt
      901 ctttcaatat gcttccggat gaagtggatc gcctcaggac ttcaaagaca aaagccgtgt
      961 tgccggtctg cctttatggc tcatgcatgt acattgatgc cattcaccgg attgcccgca
     1021 aagccgacat tccagttatc gtggatgcag cacagtgctt tggtatcagg tcactcatca
     1081 accactgtga tctgctggcg ttgagcttta atccattcaa gaatattggt tctcttggta
     1141 aatccggtgc agttctaacc ctttctcctg aactggcacg cctggcaagg caatattcat
     1201 atcacggatt tgctgaaggt aaaaagaata ttaaggccca gaactgggga ttaaacagct
     1261 gtatggataa tctgcaggct gccacgctta gtgtcaaact tcaccatttt gaaaataatg
     1321 caaaaaaacg ctgccttctt gcagcccgtt accatctttt actgtctgac ctgtcacata
     1381 acataatact tccgacagag acccatcaga atacatggca ccttttccct attctgctct
     1441 gcaaaggcga acgtgattct ctgttcgcct ttgctcgtga aaagggagtg gagttagata
     1501 tttactatcc cgtcctttca cattgcggag agcatccatt ggcaaccgga tattcccgtc
     1561 gagagcagtt cagtgattct gaaaaaatac attctgcatt acttcatctg ccgctgcata
     1621 accatatgtc gctccaggag caaaacatcg ttatcgaggt actgcatgac tattttaaat
     1681 aaagactggc ccgtcgaatt cccgttcccg acagtaatcc gcactgtggt gtgcagcgat
     1741 cttgatgaaa cttatattcc ttcggacagc gataaaaaaa aactgagtgg gatcgttcag
     1801 ctggaatcct atattacatc ccatgctgaa aaaaagggga ttctcgctgg ctggatcaca
     1861 ggaacaaatc tggattcagt ctggcgaaaa tccaggggat atatatcgcg aagccctcac
     1921 tttatatgtt gcagtctcgg caccgaattt tactgggtga aaaatggtcg tctcatcccc
     1981 tctgaaacct gggcagaaag aataagccgt tcaggctaca gtcgtcagaa tgtcaactgt
     2041 ctggttaaga ttctgatgga aaaagagatc ccccttctga aacaaccaga ggattatcag
     2101 ggccccttta aagtgagcta ttactaccct gagagcccat ctattgccag cgattttgcc
     2161 accatccagg aactggctga cgagcgacga attcgtgttg tgtttacccg ctgtaacccg
     2221 gcggctggcg atccctctgg ctgttatgac gttgaattta tccccctctg ttgtggcaag
     2281 gatcaggccg tgtcttttct gatggaggaa accgggctgc cgaaagaagc cattattgcc
     2341 tttggtgaca gtgcgaatga ttttgccatg tttgcacagg ccggaagtgg ctatctggtg
     2401 ggtaatgccg acccgtttgc agtcagccag tacggaaaca gactgaacag tgcttactgc
     2461 cacgggatac tgagtgttct taaggagcta cagtgatgat tatcggaatt gtcggctgtg
     2521 gggggattgc ccgtgcccat gtcaacgcac tttgtctcaa tccgctagta actggtctgg
     2581 ccctgtacga tgtgaatcca gacaaagtcc gacagctcag cgaaataagc tccctgccgg
     2641 taacaaactg ccaggatctg gagcagcttg ccggagtcag tcaagggttc attatctgca
     2701 caccaaacca tctccacgtt agcgtggcag aagaagtgct tcgctacaaa cagctcccgt
     2761 ttgtgtgtga aaaacccctc tcaacagatc tcaactctgc acgatatcta caggagattg
     2821 ctccaaaagg tagtatcgtg tcttttaact accgatataa ccccgtcatc gacatgataa
     2881 tgcgtctgag aaatgagagg aaattaggtg aactgcattt cttctcagca gaatttaata
     2941 aaaattctgc actgacccgc catcacctga cctggcgaga ctccgcacaa cagagcaaaa
     3001 gcagcggtgc tctgggcgat ctatcctgtc atctgcttga tttattttgt tttatagggg
     3061 aaagcccggt agtggttcat gggatcaaaa cggtaaaggg gacacgggtg ggtacaaaat
     3121 cagatggtca ggttgaagtg gatgataacg gctatgtaat gggcagttca gaaaagggag
     3181 cttatttcag agttcatgcc agtaaatcgg aaacagatca gaatttggga ctgcatatac
     3241 agcttgtatt tgaaaatggt gaaatcagat attcaaccca ccatgaaaat cgcctgctcc
     3301 tgattctatt taatgatacg aatacggaaa caattggctt cgacgctctg aaacgcttgc
     3361 ctgatccacc acgggagctt ccattctggt ctgattcgtt tattcatctt catgacgact
     3421 ggtgtgctct tattaaatat ggtcactcgt ctcctaagct ggcggacctg tcatccggcc
     3481 ttcatataca ggaaataatt gaggcattct gatatggata caattctttt aaccgggctg
     3541 tttgctgcat ttttcacaac gtttgcgttc gctccccaga gcataaaaac tatcaggaca
     3601 cgcaataccg aaggtatctc cgttgttatg tatatcatgt tcctgacagg tgtaatctca
     3661 tggatagcgt atggcattat gcgatctgat tttgccgtac tgatcgccaa tattgtcaca
     3721 ctgtttctgg ctgcaccagt gcttgtaata acacttatca accgcaggaa aaaacacgta
     3781 tgaatattgc aatcatcggc gcgggtcctg ccggcatcat ttctgcccgt aatgcgatta
     3841 aagcaggtca ctccgtggtt ctgtttgaga agaatacccg aattgggggg atctggaccc
     3901 cctggagtgg tggcgcttac cgtaatgcct gtatgcagaa ctcccgttat acatttcatt
     3961 atactggctt tccccctggc gatatcgatg aatttccagg agtggaacag gtattcagat
     4021 atctttcggc cgtagccgga gaggatgccc tccgtgagtc gacccgactg aatactgaag
     4081 ttgtttcact cagaaaagac gccggacact gggtgatccg ctgcgcttct gaaggaaaag
     4141 acacggaaga catttttgac cgggtcatca ttgcaacagg tgaactctgg caaccccgcc
     4201 ggcccccctg ccaggtgagg aaaacttctc cggaacgttg atcacgtcga gaaattatca
     4261 ggagccagag gcatttaaag gaaaaaatat cctcatcatt ggcggcggtg tcagcggtgc
     4321 ggatattgcc tcagaccttg ttccctttgc cagaagcgta agtctgtccg tcaaaaagat
     4381 gggactttat ctgccgagac aattcccgac tggcccgaat gacatgatgc actcctatct
     4441 gggcaggtgt ctgctgagcc aaatgaatta tgaagatttt atcggttatc tcgacaccat
     4501 gatgcctgac tacatgcagg cctaccgagc atccggtctg ttgcccgaca tggcgaacaa
     4561 taacgcggta catgttaacg aaaaaatcat accgaatgta gccgccggtc tgataaaagt
     4621 taaacccctg gcagagcgtt tcaccggaga aggcgctatt gagttcgtcg acgcatctca
     4681 ggaaaaatat gatgtaatca taacctgtac aggctatgaa atgcctgatt actcgttcat
     4741 tcagggattt gacagaacac agctctatga gcactttttc tggacagagg acccttctct
     4801 ggccgtcatt aatcccccgg tggataccgc cggtttcggg gctgcgtttc cctattttga
     4861 tatcatcagc cagtgggtaa tgaacgtatt ctccgggaag acttcccttc cggaaaaaga
     4921 agctatgcgc aaatggtgtg ctgagcacat ggcttcactt catgttaagc gattttatga
     4981 cagctggctg gaaaccatcc ggattgggct gctgtcaggt cttctcccgg acccggcccg
     5041 cgacttttcc cgatactgga acatcatcag cagtatggtc aaacctgcct atctggccac
     5101 tcctcccgca tttccggagc atggtatgat ggacagcctg tttgatttcc ggatcgccag
     5161 aatacgcata ctgtcagggc tgggaaacga tgctctcggt tatttactga aaaaaggtga
     5221 tatcactgac gcagaatacc gggcggccct ggaaatcgat ccccggcagt cgatttctgt
     5281 acacctgccg tattcacaga cctacctctg ataaaaaggg ccagtaacgg cccttttctt
     5341 ttttcataat attctgatca gtcttgccgg gtttcctgca taaatgcctt tgttctcaac
     5401 cggttttact acaactgccc ccgcaccgat aacagctccg ctgcatatat acggagacag
     5461 aataatggct ccactgccaa cagtaaccga atcccccagg ataatgctga tccagttatc
     5521 cggtgacgga tctggtgctc ctgaacgaaa cagatcgttg gcgaacgtca cgttatgacc
     5581 aatgaagcag ttctccccca gtgttacgtt ctcacaaata aacgtgtgcg actgtatccg
     5641 acttccacta ccaatgacac agcctttctg tatctcaacg aaggggccga caaacacatt
     5701 gtccctgagt tcacattcat aaatgttgac cggtttgaca atacggacat tggtcccata
     5761 aatcacatcc cggacagcac attccataaa tttcatactc acacgtcctt taaatacaga
     5821 tgacacttgc cggaattata cagcccagat tcagttgcca tgtttcagaa tagcttataa
     5881 gccgcacttt taccgatact cagcaacaaa tcaggtctgg atccacttca gccggaaacc
     5941 ggatctgcgc ggcgggaaac gggcaggtga tatgataatt aagcaacaca cataaattca
     6001 tagcagacat gattttaatc atcagtaatg aaataatatc tgatattatc agaaatcgat
     6061 aacacaaaaa tcatatagaa tttgattttt acctgtcaga acattatcag atggagcgcg
     6121 caggatggat gaagaaaggg tgttttcact cagctatgaa caactgaccc ggtttgcgga
     6181 aaaacgcatc cgggaatgca atctggacag ccagggcgcg atttatctct gcgagtcagc
     6241 caaagccgga gccgtgctga tcttctggca tgaactggcc ataaatggct atgcctcaat
     6301 gaatgccata aagagacagg agctcattga cgccgacttt cagcgcctca gaaacctcat
     6361 ctggccggag aatgacaggt aatgacccaa agccgccgac cgtcgccact gcagcggaga
     6421 gtgctgattg tgctggctgc ccttgatgaa aaacggccag gccccgtgct gacgcgggac
     6481 atagaacggg tgctggagcg aagcggggaa gccccggtct acgggccaaa cctgcgcgcc
     6541 tcctgccgtc gtctggagga tgccggctgg ctgcgtaccc tgcgcgctcc gaacctgcag
     6601 ctggcagtgg agctgacgga cgccggccgg gccgtggcac agcctctgct gctggcagaa
     6661 caggaccgcc tccgggcaga acagcgtgcg gcagaagtcg tcgtactgcc gctgatcccg
     6721 gcagcaggtc tgcccgcaga cggcacttct gccactgatc tggcggtgga gctgaacggc
     6781 ataacgtatc aggcctgccg tggggatttt gtggtgcgtc tcgacggcag cacctgtctg
     6841 cagttatgga ataaagaagg cagggtggta cgccgggagg gcgatccgct ggaagtggcg
     6901 cagtggctgc aggcctgtca tgacgcagga atagaagttc gcgtacagat aaatgaaagt
     6961 gccgctccgt aagaacacgc ctgtgcacgg tttacacatc atcccatggt ggcagccggt
     7021 taactttcct gcgccaccag tggaaagagg ccatgcacag tccgaacaaa aaaccccccg
     7081 tgatacttat aataatggcc tcacctgcca ccattcctga cggcccccag tagataaacc
     7141 acattgcaca tccccaggaa atgccccata agccccccat cagcaccgtg acctgccaga
     7201 acggcataaa gggtaatggc agaagccgga tacccagccg ccagagaata cgcagaagag
     7261 gaggggcata attgctccgc cacatctttt tgctgtccat cagggcaatg gcccgggctt
     7321 ttttctgctc aaaggtcaca ggagcacctc cgttccatga tgtaaacgtg acacaactta
     7381 ttgttattga ttatcattat catgatgtgg ttatgttatc acgcaccggt acacgataac
     7441 aataaaggag aaaccagatg aaaccggata tatctgtaaa ttattttctg aaaagccatc
     7501 cttcggatga attattttat tataaagttt catatatctg
//
LOCUS       DAZGSP010000173         7436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZGSP010000173 DAZGSP010000000
VERSION     DAZGSP010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.174"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            280..795
                     /gene="ehxC"
                     /locus_tag="ACHK74_004836"
                     /gene_synonym="hlyC"
     CDS             280..795
                     /gene="ehxC"
                     /locus_tag="ACHK74_004836"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFP0223515.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWLSGERKWFIDWIAPFGHNMEL
                     YKHMRKKYPYELFRAIRLDEYSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
     gene            797..3793
                     /gene="ehxA"
                     /locus_tag="ACHK74_004837"
     CDS             797..3793
                     /gene="ehxA"
                     /locus_tag="ACHK74_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFP0223516.1"
                     /translation="MTVNKIKNIFNNATSTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADDLGIEIHRTERDDTAIANQFFGTAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGGTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PNLGPLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAIADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVADKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFVTPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSNLIQFVEKDNKHYQARIISELGDKDDIVYSGAGSSEVFAGEGHD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDRDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVI
                     TDKDGREIKADKIPHNNNERSGYIKASNIASEKNMVNITSVADDINKIISSVSGFDSG
                     DERLASLYNLSLHQNNTHSTTLTTTV"
     gene            3843..5963
                     /gene="ehxB"
                     /locus_tag="ACHK74_004838"
                     /gene_synonym="hlyB"
     CDS             3843..5963
                     /gene="ehxB"
                     /locus_tag="ACHK74_004838"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFP0223517.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELGAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFITELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            5967..7406
                     /gene="ehxD"
                     /locus_tag="ACHK74_004839"
     CDS             5967..7406
                     /gene="ehxD"
                     /locus_tag="ACHK74_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFP0223518.1"
                     /translation="MRFYMKGLWDLICRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVIWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGAFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETINESFRER"
BASE COUNT         2392 a         1148 c         1645 g         2251 t
ORIGIN      
        1 ttaataggtg ggttttactt tgctgcatcc tgtttgatat ttctttgatg agaagtttgt
       61 cgcagggtga ttcatctgaa tgttataata taacctatct aggtgtgcat aagaatacat
      121 agctaatgta ttactgtttc taatgttgtt tttttgtagg agggattctc cttgcggtag
      181 ctttttgttt ttagatgctt attgcttaaa gaatataatt tttatgcttt tatattgatt
      241 tctttacatt tactgagttt tcttatttat ttaaacatta tgaaatctaa tgcttttgac
      301 gtcatgggga aggttgcatg gctatgggcc tgttctcctc tgcataaaaa atggccattg
      361 tctgtttttg ctattaatgt catacctgct attcagacaa atcaatttgc tctcttaata
      421 aaggatgagc ttcctgtagc tttttgtagc tgggccagct tagatctgga gtgtgaggta
      481 aaatatataa atgatgtaac atctttgtat gcaaaagact ggttgtcggg agaaagaaag
      541 tggtttattg actggatagc tccatttgga cacaacatgg agttatataa acacatgagg
      601 aaaaagtatc cttatgagtt attcagagca ataaggttag atgagtacag taaaacaggg
      661 aaaatagctg aatttcatgg aggggggata gataaaaaac tggcatcaaa aatattcagg
      721 caataccatc atgaacttat gtctgaagta aaaaacagac aagattttaa ttttaatatt
      781 gagaaagaaa actaatatga cagtaaataa aataaagaac attttcaaca atgcgacatc
      841 gactacaaaa tcagcattca atacagcatc atcaagcgta cgttccgctg gaaaaaaact
      901 catattatta atacctgata attatgaagc tcagggcgtg ggtattaatg agttggtcaa
      961 agctgctgat gatcttggaa tagaaataca ccgtactgaa cgagatgata cagcgattgc
     1021 aaaccagttt tttggtacag cagaaaaagt tgtaggatta actgaacgtg gtgttgcaat
     1081 attcgcacca caacttgaca aacttctgca gaagtatcag aaagttggga gtaaaatagg
     1141 aggaaccgct gaaaatgtag gtaataatct gggaaaagcc ggaacagttc tctcagcact
     1201 acagaatttt acggggattg ctttatctgg catggctctt gatgaattgc tgagaaaaca
     1261 acgggaagga gaggatataa gtcagaatga tattgccaaa agtagtattg aacttattaa
     1321 tcagcttgta gatacagtat caagtataaa cagtaccgtt gattcatttt ctgagcagct
     1381 taaccagctt ggctcatttt tatccagtaa acctcgctta agttctgttg gtggaaaatt
     1441 acaaaattta ccgaacctgg gccccctggg ggatgggctg gatgttgtct ccggaattct
     1501 ttctgctgta tcagcaagct ttattctggg aaacagtgac gcacatacag gaacaaaagc
     1561 tgcagcgggt atcgaactga caactcaggt tcttggaaat gttggtaaag ctgtttcgca
     1621 atatattctg gctcagagaa tggcacaggg attatcgaca acagctgcaa gtgcgggtct
     1681 gatcacatcg gctgttatgc tggctatcag tcctctttct ttcctggcta ttgcagataa
     1741 atttgagcga gctaagcagc ttgaatcata ttctgaacga tttaaaaaat tgaattatga
     1801 aggggatgct ttactcgcag cctttcataa agaaaccgga gctatagatg cagccctgac
     1861 aacaataaat actgtcctga gttctgtatc tgcgggagtt agtgccgcct ccagtgcatc
     1921 cctcataggg gccccgataa gcatgctggt gagtgcatta accggtacga tatctggcat
     1981 tctggaagca tcaaaacagg ctatgtttga gcacgttgca gataaattcg ctgcccggat
     2041 caatgaatgg gaaaaggagc atggcaaaaa ttattttgag aatggctatg acgcaagaca
     2101 tgctgcgttt ttagaagatt ctctgtcttt gcttgctgat ttttctcgtc agcatgcagt
     2161 agaaagagct gtcgcaatca cccagcaaca ttgggatgag aagatcggtg aacttgcagg
     2221 tataacccgt aatgctgatc gcagtcagag tggtaaggca tatattaatt atctggagaa
     2281 tggagggctt ttagaggctc aaccgaagga gtttacacaa caagtttttg atcctcaaaa
     2341 agggactata gacctttcaa caggtaatgt atcaagtgtt ttgacatttg taacaccaac
     2401 atttacccca ggagaagaag tcagagaaag aaaacagagt ggtaaatatg aatatatgac
     2461 atctcttatt gtaaatggta aggatacatg gtctgtaaaa ggcataaaaa atcataaagg
     2521 tgtatatgat tattcaaatt tgattcagtt tgttgaaaag gataacaaac actatcaggc
     2581 gagaataatt tctgagctcg gagataaaga cgatatagtt tattctgggg caggctcatc
     2641 agaagtattt gctggtgaag gtcatgatac cgtatcttat aataagacgg atgttggtaa
     2701 actaacaatt gatgcaacag gagcatcaaa acctggtgaa tatatagttt caaaaaatat
     2761 gtatggtgac gtgaaggtat tgcaggaagt cgttaaggaa caggaggtgt cagtagggaa
     2821 gcgaacagag aaaatacaat atcgtgattt tgaattcaga accggtggaa ttccttatga
     2881 tgtgatagat aatcttcatt ctgttgaaga actcattggc ggaaaacatg atgatgaatt
     2941 caaaggcggt aagtttaatg atatattcca tggcgcagat gggaacgatt atatcgaagg
     3001 taattatggt aatgatcgac tatacggcga tgatagggat gattatatat ccggaggaca
     3061 gggagacgac cagttatttg gtggtagtgg aaacgataaa ttgagtggag gggatggtaa
     3121 taattatctg acaggaggaa gcggtaatga tgagcttcag gcacacggag cttataatat
     3181 tctgtcaggt ggtactggtg atgataaact ttatggtggt ggtggtattg accttctgga
     3241 tggaggggaa ggtaatgact atctgaatgg tggttttggt aatgatattt atgtttatag
     3301 gcaaaactat ggtcatcata caattgcaga tgaaggaggt aaaggagatc gtctgcactt
     3361 atctgatatt agctttgatg atatcgcatt taagagagtt ggaaatgatc ttatcatgaa
     3421 taaagccatt aatggtgtac tttcatttaa tgagtcaaat gatgtcaatg ggataacatt
     3481 taaaaactgg tttgcgaaag atgcctcagg agcagataat catcttgttg aggttataac
     3541 agataaagat ggtcgtgaga taaaagctga taagatacct cataataata atgaacggtc
     3601 aggttatata aaagccagta atatagcatc tgaaaaaaac atggttaata tcaccagtgt
     3661 tgccgatgat attaacaaga ttatttcttc agtttcaggg ttcgattcag gtgatgaacg
     3721 attagcatct ttatataatt tatccttaca tcaaaacaac acacattcaa caactttaac
     3781 gacaactgtc tgagactaaa agcggaggtg atggccattc caggaggaat ggtcatcgtt
     3841 atataatgag taaatgtagt tctcataata gtctgtatgc actgatattg cttgcacaat
     3901 atcataatat aactgtcaat gctgaaacta taaggcatca gtataatacc cacacacaag
     3961 attttggggt gactgaatgg ttactggcag cgaaatccat tggcttaaaa gcaaaatatg
     4021 tagaaaaaca tttttccaga ttgtcaataa tttctttacc tgcgttgata tggcgggatg
     4081 acggtaagca ttatatatta tctcgtgtta ctaaagattc atcacgctat cttgtttatg
     4141 atccagaaca gcatcagtca ctaactttta gtcgggatga gttcgaaaaa ctgtatcagg
     4201 gaaaagtcat tctggttacg tcaagagcaa cagtagtcgg agagttagct aaatttgatt
     4261 tttcctggtt tatcccctct gttgtgaaat acaggaggat tttacttgag gtgttaactg
     4321 tttctgcttt tattcagttt cttgcgttaa taacacctct tttttttcag gttgtaatgg
     4381 ataaggtttt agttcaccgg gggttttcaa cgttaaatat tatcacaata gcatttatta
     4441 tagtgatact ttttgaagtg atattaaccg gagccagaac ttatattttc tctcatacta
     4501 caagtcgtat tgacgtcgaa ctgggtgcta agttattcag acatttgctt gcattgcctg
     4561 tttcatattt tgaaaatcgc agggtcggag agaccgttgc cagagtaagg gaactggagc
     4621 aaattcgtaa ttttttaacc ggacaagcgt tgacatcagt tcttgatctg tttttttctg
     4681 taatattttt ttgtgtcatg tggtattaca gtcctcaatt aacactggtt atattattgt
     4741 cactaccttg ttatgttata tggtcattgt ttatatcacc cttattacgt cgacgtcttg
     4801 atgataagtt tctcaggaat gcagaaaatc aagcttttct tgtcgaaacg gtaacagcaa
     4861 taaatacaat taaatccatg gcagtatcgc cacagatgat tgctacatgg gataaacaac
     4921 tggccgggta tgttgcttcc agtttcaggg taaatctggt tgcaatgaca gggcagcagg
     4981 ggatacagct gatacagaaa agtgtaatgg taattagtct atggatggga gcacatcttg
     5041 tcatatcggg agagataagc atcgggcagt taatcgcgtt taatatgctt gcaggtcagg
     5101 tcattgctcc tgttatcaga ctggctcatc tttggcagga ttttcagcaa gttggtattt
     5161 ctgttgagcg ccttggggat gtattaaata caccggtaga gaaaaagtca ggcagaaata
     5221 tactgccgga aattcagggg gatatcgaat ttaaaaatgt cagatttcgg tattcttctg
     5281 acggtaatgt tattttgaat aatattaatt tatacatatc aaagggggat gttatcggta
     5341 tagttggccg ttctggttca ggaaaaagta cattaacaaa actgcttcag cgcttttata
     5401 taccagagac cggacagatt ttaattgatg ggcatgattt atcacttgca gatccagaat
     5461 ggttacgacg ccagattggt gttgtattac aggaaaatat actattaaat cgtagtatta
     5521 tcgataatat tacattagct tctccggctg tatctatgga acaggctatt gaggcagcca
     5581 gacttgcagg tgcccatgat tttattacag aactaaaaga agggtacaat actattgttg
     5641 gagaacaggg cgttggtctt tcaggagggc agcgccaacg gatcgcaata gcccgggctc
     5701 ttgttacaaa tcctcgaatt cttatttttg atgaagcaac cagtgctctt gattatgagt
     5761 cggaaaatat aataatgaaa aatatgtcaa gaatatgtaa gaacagaacc gtaattatta
     5821 ttgcacacag gttgtcaact gtaaaaaatg caaacagaat aattgttatg gataatggat
     5881 ttatttctga agatggcaca cataaagagc ttatctccaa aaaagacagt ttatatgcat
     5941 atttatatca gttgcaggca taacgtatgc gcttttacat gaaaggttta tgggatttga
     6001 tttgccgtta taagacggtt ttttctgatg tctggaaaat tcgtcacaca cttgatgctc
     6061 ctgtgagaga aaaagatgag tatgcatttc ttcctgctca ccttgaactc attgaaacac
     6121 cagtatccag acgttctcat tttgttatat ggagtatttt attatttgta attatatctc
     6181 ttctcttatc tgttcttggg aaagttgaag tggtctcagt agcaaatggt aagtttactc
     6241 atagtggaag aagcaaagaa ataaaaccga tagagaatgc gattgtagaa aaaataatgg
     6301 tgaaagatgg cgcttttgta aaaaaaaatg atccattagt tgaattgacg gtgcctggtg
     6361 ttgaatctga tattttaaaa tcagaagcat ctttgttgta tgaaaaaaca gaacaatatc
     6421 gctacgcaat cctttctgaa tcaatacaac ggaatgagct tcctgaaata aggataacag
     6481 attttcctgg cggagaagat aatgcaggag gtgaacattt tcagagggtt agctcattaa
     6541 taaaagagca attcatgaca tggcagaaca gaaaaaatca aaagcagctc acattaaata
     6601 aaaaaatagt tgaacgggat gcagcgcttg ctcgtgttag cctgtatgag catcaggtat
     6661 cacaggaagg aagaaaactt aatgatttta agtatttgtt gaataaaaaa gctgtttctc
     6721 aacattcagt tatggagcag gaaaatagct atattcaggc aaaaaatgaa catgcagtct
     6781 ggcttgcaca ggtttctcaa cttgaaaaag aaatagaact tgtgcgggaa gaactggcac
     6841 tggagacgaa tatcttcaga agtgaaatta tcgagaagca cagaaaatca acagataaca
     6901 ttgtgttact ggagcatgag cttgaaaaaa acaggcagag aaaagcatca tcttttatta
     6961 aagctcctgt gagtggtact gttcaggagt taaatataca tacagaaggt ggtgttgtca
     7021 caacagcaga aacgctgatg attattgtcc ctgataatga tattctcgaa gtaacagcat
     7081 ctgtacttaa caaggatatc ggttttatac aacctggaca ggaggtcgtt attaaagtag
     7141 atgcatatcc atatacacgt catggttacc ttacagggaa agtaaaaaat ataactgcag
     7201 attctgtttc tgttccggat acaggacttg tatttaacgt gattatatct gttgatcgga
     7261 atgatataca gggagaaaga aaaaaaattc ctgttacagc tggaatgact gttatggcag
     7321 aaataaaaac aggagtgcgt agtgttatca gctatcttct tagcccatta aaggaaacta
     7381 ttaatgaaag tttccgtgaa cgttagtata taaataacat attggctttt tatata
//
LOCUS       DAZGSP010000174         7334 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZGSP010000174 DAZGSP010000000
VERSION     DAZGSP010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.175"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..357
                     /locus_tag="ACHK74_004840"
     CDS             <1..357
                     /locus_tag="ACHK74_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFP0223519.1"
                     /translation="QLYPQVQWVQSFADERCGCLGVVYQASNFDYVGSHESTFYELDG
                     EWYHEICRNAIKRGGQRGEHLRANIDRASVHKFRQFRYIRFLNKRARKRLNTKLFKVQ
                     PYPKPQTVKTGLKEIG"
     gene            complement(485..4207)
                     /locus_tag="ACHK74_004841"
                     /pseudo
     CDS             complement(485..4207)
                     /locus_tag="ACHK74_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element core protein"
     gene            complement(4283..6424)
                     /locus_tag="ACHK74_004842"
                     /pseudo
     CDS             complement(4283..6424)
                     /locus_tag="ACHK74_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
     gene            complement(6634..7152)
                     /locus_tag="ACHK74_004843"
     CDS             complement(6634..7152)
                     /locus_tag="ACHK74_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP0223520.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
BASE COUNT         1377 a         2343 c         1920 g         1694 t
ORIGIN      
        1 cagctttatc cgcaggtaca gtgggttcag tcgtttgcag atgagcgttg tggctgtctt
       61 ggtgtcgtgt atcaggcaag caattttgat tatgtaggca gccatgaatc aacattctat
      121 gaacttgatg gcgagtggta tcacgagatt tgcaggaatg ccattaaacg aggcggacaa
      181 cgaggtgaac atctgagggc taatatcgac agagccagtg ttcataagtt ccgacagttt
      241 cgttacatcc ggtttctgaa caagagggcc agaaaacgcc tcaacacaaa gctattcaaa
      301 gtccagcctt acccaaaacc acaaactgtt aaaaccggtt tgaaagagat tgggtgagtg
      361 cttaaaaact cattctaaaa cgcaaaggaa aagcgccgat ttagtatcaa ataagttgcg
      421 tatcggcgtt tttcagcggt atcaaatgag atgcaaatcg gtatcaaaaa aatcgccgcg
      481 ctataagatt ccattcacct ctcagcccta tcgggtcctg ggtgatatat ctcccctgca
      541 acggatcata gtaccggttg cggttgtagt acagccccga ctcctcatca tactgctgtc
      601 ccggcagacg gagtgactgc tgaaggtgct gcgcgctttc ctcgcccagc aggtttcccc
      661 actcgtcata ctccccctgc cacgccgttt ccccttccgg gctgataagc gccagcggca
      721 gcccacggtg gtcacagtgg taaagatgaa gtttcctctc cgggatgtat tccgcctcca
      781 gttgcgcggc catctgttcc gccgtcagcc cgcactgcgc aagccactgc tggctttctt
      841 cactcacgct gccctgccgc agttcccgct ccagcctccc cagcatcacc gccagctccg
      901 ccggtagcgt cacacccgtg tcctcctgca acacctccgc caggctacgg tgccgcgcct
      961 tcgcctgttc accattctct gtttcgattc tcaggagcgg cgtgaagctt cccggctggt
     1021 ataccgtctg gattcttgtc tgttgggtct gtacagtggt cagcctgtca ccgtcccacc
     1081 cgtaccaggt ctcctccggt ttacgcgaca gcgacatcca ccccgtcagg tcacgctccc
     1141 gccgccacac ccgttttccc gtccggcggc ccagcgggtc atagaggtag cggctctcca
     1201 cctgcggctc accgtgctgt atccgcgtgt agaacaccag acggtgctgg ctgtcgtagt
     1261 ggtagtggtg ggtccgctcg tcgtgcatcc gtatcacccc ttccgggatg cggtccgtct
     1321 tctccgccag cctgccgtat tcatcgtagc ggtagacata gtgcgcatct tccgcgatgc
     1381 ggttatccgg ccacgccgtg agggtgctgt ccgggtgcag ttccgggtcc ggcagccggt
     1441 ttcctgccgg gtccgtcgca tacgggatat cgatatccag gttcgctgcc gtagtgtgga
     1501 cgcccgtcag ccttcccgtg ccgctgtaac ggtactcccg gcactcctgc gggccgctga
     1561 tgcgcaccag ttgtccgtta tcgttccacg tatagtcaca gtcaagctgc ggaaggttca
     1621 ggtgcctgct ctgtagctgc cctgtgaggg tgtacgccgt ggcctgttcg taccccgctg
     1681 tactgcccgc cccgccgaag ctacgggccg tctcgcggtg cagccggtcc cgcgtgtact
     1741 cgaccagcgg cgttccgccc agcttcatcc ccgcaagata accgctgcca tacgtcagcc
     1801 actccaccgg cggcagaccg tccggctcct gacgggtcgc cagcccctgt tcgctgtacg
     1861 catgccccgt ctcatgctcc cacagcatct cccctgtctc cgggttctcc accgtctgcc
     1921 gttcacccgt caggcggcct ttatcgtcat agccgtagtg gaccgacacg cggtggcctt
     1981 cacaggtatg gctgagggtg gttaaccacc cgtgctcatc atactgccac tgctctgccg
     2041 ggtcgccgtt caccgtccgg tgcgtgatgc ggtccgacgc atcgtagtgc cagagggtga
     2101 taagcccctc gtcttcactc tgcgtgagtt ttccggtcag gtcatagtgg taacgttgcg
     2161 tccggccgtc aaaaccgcgc tgttcagtca gcctgtccac cgggtcatac cggaacgtgg
     2221 actggctgcc gttctcgttg gtcagcacgg tgatgcgccc ggcagcgtca taccccatgc
     2281 tgcgcgtaag accgccttcc gtcacactca ccggacgccc gcgtttatca tattcgatgg
     2341 tgctgcgttt accgtccggt gatatcgttg ctgtcaggtc gcctgcggcg ctgtactcat
     2401 aacggatttc acgcccctgc gcatccttct gactgaccag ttgcccacgc gggttataac
     2461 tgctgtaagt gctgatgcct tcctcccggt gaacggcgat ttgttgaccg taccggtcgt
     2521 actcataccg cgtcgtgtac cccgagcagt ccgtgaaggc cagcagttga ccgtaacggc
     2581 tccatgccat ctgttttgta ctgcccgtcg cgtcctgtat ccctgtcggc agctcactcg
     2641 ccgggtcatc atagctgtac cgcgttgtct caccgctgcg cgaggtctcc gccgtcagcc
     2701 ttcccttctc atcatactcc cggctgctgc gaagcccgtc cgggtacgtc accgacgtca
     2761 cctgccgctg gctgttatag ccataccgta ccgtcctgcc gtccggcccc gtcaccgccg
     2821 tcaccgcacc cgacgccata tgcaggctgt actccgtccg ccgccccgcc gcatccgtct
     2881 gtgccttcag cctccccgcc tcatcatact cgctgcgggt aatgctcccg tccgcatgtt
     2941 ccttcttcac cacccgcttc aggccaccct cgccttccgt gtacagcacc tcccgccggt
     3001 tcaggctgtc cgtgatgatt acccggctct ctccgtactc aaagcggtag tccagcccct
     3061 ccgggttgac ctgctccgtc acccgaccgg tatcatcata ccggtagcag ctctccggcc
     3121 tccccgcata atggtgcgcc accatccgcc cggcgtgctc cgcatcataa gtaaacccgc
     3181 gcacctgcgt tccgctgcgg tcatacaccg cccgcagttc accaccggcc gtgtacgtgt
     3241 agcgcgccag cggcgcagtc ggctgttcat ccgggtatgc cgggtcgtgt gtcagccata
     3301 ccgcctccag ccggataccg ttatccgtgc cgtatcctgt accggcgggc agcgtgtcgg
     3361 ggaaaaccag agaagaggaa gcagaacggg gaccggcagg ggaagataaa gacgtggcgc
     3421 gctgtttacg gaatacttcc gcccgctgtg cctgtgtggt cagcaccagg tgaaaacggc
     3481 ggcccgcgcc gtccgtcacc cccgtcaccg cgcccgccac atcaccctcc gccgcccggt
     3541 gaaaggccag cgtccgcccg aagccgtcca ccacgcccgt cagcacccgg tatgcgggcg
     3601 gctccggcgg cagcacctcg tccgcccccg gcacccgctc cggccagctg agtatccacc
     3661 acggcccctg aaggctgttt gtcgccaggt acacatgcgg actcaggcga acatcttccg
     3721 gcagcacctg ccagagcgcg cttagcggct gcgaactgtg ctgcgccgct actccgcccc
     3781 gcgccagcca gagtgactcg ctgcggctgt agcttatctc gccgggaaac agcggctcaa
     3841 agtgaatgct cctgccgccg ctgtcgttga gtatcaggcc ttcatcgcgt atctgtaagc
     3901 ggatatcgaa gggcgctttc cagccgggac caaacacccc caccggcgcg ggtgttctgg
     3961 tccggtagct gctgtaggcg cgggaaagaa taaacggcag cgggccgggc agcgcaaggt
     4021 ccgtctcgcc cggcagcacc ttcgcaccca gcagcgggtt caccgggtta gcataggtaa
     4081 tccctcccgg acataccgaa caggccactc ccgtcggcgc acctatcagc acccccgctg
     4141 aaccctgcac aatatccagt cccttgcggg tcatatcacc ctgccgcgcc gccggttttc
     4201 cgcccatgaa tcaattcctt ctgcttttaa tatggtttat ctggtcactg caccagttcc
     4261 gttttaattt tttgcacctg actcaaaatt gccgcatatc cggcattccg gggggtaaat
     4321 ctttcggcac aggcgcatcc ggtacaggtt gtgtggcacc acagttccag ttaatcactg
     4381 gcgcatcacc gttcacatcc gactcccctt ccagatgaat gtgcgtacca ttgagaaata
     4441 tgcttccgtc cttcgtcagc accagccttg ccttaccaca gcagagttca agatgctcac
     4501 cggcagaata aactgctgtt tgtccggtgt tttccttcat tgtcttgccc accgagaagg
     4561 tgacgttatt ccccacattg acgctgtagc ccattcccac catcagtgat ttatgcagcc
     4621 ctacctgtga ggactgcaac aacgccaccg aggtattcat aatgccgcct accgtcgtct
     4681 ggtaagccac acccaccgtc agcgcacgaa tgatccccac cttttcaacc tggtttgatc
     4741 ccactttgat aatctggtta ctgcccaccg tttccacctg gttgacacca accgtctgta
     4801 tctgattaac gccaatgttc tggatctagt tattggtaac ggtgatcgcc tggttgttac
     4861 ctattttttc ggcatggttg tttatcacat cagtggtgcg gttattcagc acctcggtgt
     4921 tcatgttctt ctgcgcgtgg atgtagacct gttctttccc tgtcgcatcg tcaaacttca
     4981 gttcattaaa cccgctgccc ttataggttt tcgagcggat cgtcatctgc gtcttcgtcc
     5041 ccggcaggct gccgggggtg cggttttcct ggtggtaggt acgccccata atgatcggct
     5101 ggtccggatc gccgttgagg aagtccacaa tgacctcctg acccacacgc ggtatcgcca
     5161 ggttaccaaa gccggtgctt gcccacgcct gtgccacacg gatccagcat gaactttcct
     5221 ggtttgacgg gttatagcgg tcccagttaa atttcacccg cacgcggcca tgttcatcac
     5281 agaagatttc ctcgcctgcc ggtcccgtca cgacggcgct ctgcgggccg tccaccagcg
     5341 gtttcagcaa cggctgtggc cgccatgtcc ggtctgccgg gattaccgca aagtggttat
     5401 ccagcgtggt gcctgaaccc ctgcgtcccg gtaccgcctg cggctgttcg ccgtgaagat
     5461 cgctcgccac cacctgccat tcccggttca ggttcgcctg cggatgcccc gtcagcacaa
     5521 ttcgccgtcc cggccatatc tccggcgaac ggcttgttcc gcgcgccact tctgcgttgt
     5581 tgcgccagcc atccatctgc cagcgggcaa agttctgccc gtgggcaccc ttgaaacgtc
     5641 cggggtagtc atacacttca tactgtgtgc gctggtaatc ctggtactgg ccttcctgat
     5701 caaaacgccc tgcccagccg ggtcgtttaa aggtgtagtc tttggtcacc acggaagaag
     5761 ggcggatttg tgcgctgtag cgaaactggc tgatgcagag ggtgctcacc tcggtacggg
     5821 tgttcggatt ccaggggatc tcaaaggact ccggcagata acgcacggta tcgcacagga
     5881 ccaggctctg gtcggtactt ttttgcgcgt gctcctcata aaagaagatg ccttcctccg
     5941 ccgccatccg gcacaggaaa tcgtaatcag tctcaccgta ctggacacaa aactcacggg
     6001 aaggatgtgg ctcgctgaac agcgggctcc actcggtcac cccgttttcc tgcaatatcg
     6061 tgccgaggat gctttcgatg tcctcattct ggaagatacg gaagttctgg cgcagccctg
     6121 tgcgccacag cggtgggcac accttcatgc tgtacagcat ctggtttttg tcgttctccc
     6181 ccagttcaaa ccaggtcacc atacctttca cccggcgctg tacgtcatcg ccctgccata
     6241 tcgtcaggta ggccattttg tcgaggatct gctggaattc aagggagaga aactgctggc
     6301 tgaccagaga gagatcgagg gaaaaaagcg aagagagtga ctggttcaga tgaaaggaga
     6361 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag cgtgaaacgt aatccggttg
     6421 acatagcaac ctccggggtt aaggaaaaac agacagttcg ctgttaaagc ggctgtgaca
     6481 gtacagtgtt acgcttgcgt tcaggcacgc taccgccgaa gtttccggca gccgccatct
     6541 cccgtgaacg taacccctta ctgacaacct ttctttctac ggcgtaatac aaaaacagca
     6601 tccggcctga tagccggatg ctgttggctt aacttatgct tccagcggtg cgcgccagtc
     6661 gtcggcaccg gaggtgccag atttgacgtg ttcccattca attttgcggt aggccagtga
     6721 aacttcgagc aactgggtga actcgcgttt cgccgggtcc tggcaatgcg gcatatgcag
     6781 tttcatatcg acaatcgtcg aatcagtcag gcgggtagtg aaataatgct cctgtttgcc
     6841 ttccacagag gtacgccacc agtgaagttc agtggttggc agcatttcgc cggaggccag
     6901 tgcgttgtac atcagcggaa ccgctttgtt cagcgccacg gtaaaaatga aaggcttgtg
     6961 agcacgctga cccgcaggct gaccggactg aggatcggtc gggacagtaa cgttatgcag
     7021 gaattcctgc accagcatct cgtcttcgtg cccctgcaca tagatgttgc cgacagaatc
     7081 agcggtaaat gcacctgcag taatatttcc ctgggtctga ccagtgatgg agatataaca
     7141 cggtgttggc atttgtaaac tccttgttaa cgtggttaat tactacttac aatgacctgc
     7201 cgttaatccc agacaggcag atgcagctca cttcacggaa ccgcatcatt tttcagtatt
     7261 ttttacatac aaaccgagct agaatttacg gttgtcgtaa aaactatttt taaaatcatt
     7321 aaatctctta ttca
//
LOCUS       DAZGSP010000175         7319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZGSP010000175 DAZGSP010000000
VERSION     DAZGSP010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.176"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(35..859)
                     /locus_tag="ACHK74_004844"
     CDS             complement(35..859)
                     /locus_tag="ACHK74_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFP0223521.1"
                     /translation="MDKPKAYCRLFLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(913..2631)
                     /gene="proS"
                     /locus_tag="ACHK74_004845"
     CDS             complement(913..2631)
                     /gene="proS"
                     /locus_tag="ACHK74_004845"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFP0223522.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2743..3450)
                     /gene="tsaA"
                     /locus_tag="ACHK74_004846"
     CDS             complement(2743..3450)
                     /gene="tsaA"
                     /locus_tag="ACHK74_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFP0223523.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(3447..3851)
                     /gene="rcsF"
                     /locus_tag="ACHK74_004847"
     CDS             complement(3447..3851)
                     /gene="rcsF"
                     /locus_tag="ACHK74_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFP0223524.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(3969..4784)
                     /gene="metQ"
                     /locus_tag="ACHK74_004848"
     CDS             complement(3969..4784)
                     /gene="metQ"
                     /locus_tag="ACHK74_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFP0223525.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4824..5477)
                     /locus_tag="ACHK74_004849"
     CDS             complement(4824..5477)
                     /locus_tag="ACHK74_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFP0223526.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(5470..6501)
                     /gene="metN"
                     /locus_tag="ACHK74_004850"
     CDS             complement(5470..6501)
                     /gene="metN"
                     /locus_tag="ACHK74_004850"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFP0223527.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            6689..7261
                     /gene="gmhB"
                     /locus_tag="ACHK74_004851"
     CDS             6689..7261
                     /gene="gmhB"
                     /locus_tag="ACHK74_004851"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFP0223528.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT         1800 a         1893 c         1854 g         1772 t
ORIGIN      
        1 cgcatccggc attttgtgca cgaggctggc tttatcactg agtcagccca acaaagcgac
       61 ccgccttgat ataaatccca ggcttcaggt gcccaacata ctgtaactgc gtttctggct
      121 taaacgcaga gacatcacca atacgcatat gcatcagatc ggcagggcta atccagcccg
      181 attgcgccag tgtaagcgga tgcccggctt tggcaaatgc atcagtgaca aattccgaac
      241 aaaaccacga ctttttgtct ccttcgccca cactgcttaa ctgcgctttc gccaggccgc
      301 tgacgcactg ttggcgaaaa tcctcggaga acggattcag tgagcacatc tggcgagtca
      361 ccataaaggg aataaattcg acaatgccgc gatagttata accgctatct ttgattttat
      421 tggcaaacgc ggtgatatct gtggcttgtt gcggggtaag atccgggact cgtaagacga
      481 aaagcttatc actatgcttc atcgcttttt taagggaaac aatctggacg ccagcgcctg
      541 tcgcttctgc aacgttatta tcacccagat agattgcaac gtgactcaca gaggaagtgc
      601 tgaagacgcg gattccaaat gaggttaccc caaggcttga ggagaacagc aaatcgccgg
      661 gtttgagatc aggtgccgtt atttctttta ttgattgttc ggtgaaagag ctttgatgct
      721 ggaatttgac agcccatgtt tttgcctcgg catctactgc tgtggctgat gggtctggct
      781 ggctgatatc aacggtacaa gccgaaagta aaagaaagct ggggagaaac aggcggcagt
      841 acgcctttgg tttatccatt ttatacaatc catgtaaaaa aagggccctg aaattcagga
      901 ccctttctgg catcagcctt taatctgttt caccagatat tcgacgatgt caccagtctt
      961 aattaactgt ttctcgccgt tgcgacgata tttatattcg atatcgtcgt tgtcgaggtt
     1021 acggtcgccc agcacaatag tgtgcggaat accgatcagt tccatatcag caaacatcac
     1081 gcccggacgc tctttgcggt catccagcag cacttcgata ccttgtgcac gcagttcgct
     1141 gtacagtttc tcagcaagct cttgtacgcg gaaggatttg tgcatgttca tcggcagaat
     1201 cgccacctgg aacggcgcga tagcgtcagg ccatacgatg cctcgttcgt cgtagttctg
     1261 ctcaatcgcc gcagctacca cacgcgttac cccgataccg tagcaaccca tcgtcaggat
     1321 ttggttacgg ccatcttcac cctgtacgga ggctttcagt gcttcggagt acttggtacc
     1381 cagctggaag atgtgaccaa cttcgatacc acgtttgatc agcagcgtac cctggccatc
     1441 cgggcttgga tcgccagcca ccacgttacg gatatctgca acttccgggg tagcgacatc
     1501 gcgatcccag ttgatgccga agtagtgttt accatcgatg ttagcaccag cagcgaaatc
     1561 actcatcgcc gcaacggtac ggtcaatcac caccggaatc ggcatgttta ccggacccag
     1621 tgaacccgga ccggctttaa ccacggcacg aatttcttct tcggtcgcga aagtcagcgg
     1681 gcttgcaacc tgcggcagtt tttctgcttt aacttcgttc agctcgtgat caccgcgcac
     1741 cagcagcgca accagcggga agctactgcc ttcaaccgct ttaaccagca gagtcttaac
     1801 cgttttctca atcggcagat tgaactgttc aaccagttcc gcgatggttt tcgcgttcgg
     1861 cgtatcaacc agcgtcattt cctgggtagc agcagcgcgc ggttctttcg gcgcgatagc
     1921 ttctgccagt tcaatgttcg ctgcatagtc agaggtgtcg gagaagacca catcgtcttc
     1981 accgctctgc gccagcacct ggaattcgtg agaggcgctg ccgccgatag aaccggtgtc
     2041 ggcttgtacg gcgcggaaat ccagccccat gcggctgaag attttgctgt aggccgcata
     2101 cattgcatcg taggtttcct gcagggattc ctgagaagta tggaaagagt aagcatcttt
     2161 catcaggaat tcgcgggaac gcatgacgcc gaaacgcgga cgcacttcgt cgcggaactt
     2221 ggtctggatc tgatagaagt tcagcggcag ctgtttgtaa gagctaagct cgttacgaat
     2281 caggtcagtg ataacttctt catgagttgg gccgagtacg aacggacgct cgccacggtc
     2341 aacaaaacgc agcagttccg gaccgtactg ttcccaacga ccactctctt gccacaaatc
     2401 agctggctga accaccggca tcgacacctc gatcgcaccg gcgttgttca tctcttcacg
     2461 cacgatgttt tcgacttttt tcagaacgcg cacgccggtc ggcagccagg tatataaccc
     2521 ggaggccagc ttgcggatca tcccggcgcg cagcatcagc tgatggctga tcacctcggc
     2581 gtcggcaggt gtctccttga gagtggagag caggtattgg ctagtacgca tgttgttacg
     2641 gttccagttg gaaggtagaa caggctcaag gcgagcctgg gacaaaaaaa agtgatttag
     2701 tttaccagtg caaaagaaat gtcaaaagag aagggcgtga atttaacgcg gttccagcgc
     2761 aaagacttca aaacctgcgt cggtgacgcg ccagcgaacg ttaaaatcat gtagccagac
     2821 ggcataggtt ttgcccgttt cctcaccttt acgataggcc gggcgtgggt cctgcgccag
     2881 tacttcgcgg ataaacagcg ttaactgcgg ataacgcttc tccagcgtca aaagctgctt
     2941 ttcgacctct gcggtaaaac tcaccgccat ctctgccgct ggcgcacttt gcgcatagct
     3001 ggcactggca tcgggaagcg attcggcaaa ggggagatac ggtttgatat ccactaccgg
     3061 cgtaccatcg accagatcca gactgccgag cttcagaatc acgctgtctt tatggcaaac
     3121 aacctctttc agctctacca gcgacatacc aattgggtta gggcggaaag tagagcgtgt
     3181 tgcgaaaacc cccattctgg cgttaccgcc gaggcgcggt ggacgcacag tcggacgcca
     3241 gccgccttcc atcgtttgat gaaagacgaa aaggatccat aaatggctga acgcttccag
     3301 gccgcgaacg gcgtcggcct ggttgtaggg agcaatgaga tgcagttcgc cgttggcgct
     3361 ttttaccaga cctggctggc gcggaacggc gaatttttct ttatagggcg agcgaataac
     3421 gcctatttgc tcgaactgga aactgctcat ttcgccgtaa tgttaagcgc agagccgata
     3481 catacagcct gacgatagca gcctggcgta ccgctggtga cttcgcagct atgcagtaat
     3541 accgcattgg ctttcatttt agaggcgttg atttgcatcc gcttacgcgc ggttggaatg
     3601 ctcggcggag agtcctgatt agaggcctgg caagagtcgc cactgacttc accgagatcg
     3661 cggaacggtt tgccgactaa ttcttctgca ttggtataaa ttcggaccgg cgtggcgcgc
     3721 ggcgctttcg gttttgcagg ctccgctttc ggctggggtg cagtgctttg aacgggttcg
     3781 acaggggatc tgcttaacat ggaacagccg cttagcatga gtgctactaa acagatcggt
     3841 aaagcacgca tagtatttcc tcaatgtatg atcaaaacgt caatattgaa tcaggagctt
     3901 gtaaaaatga caagacgggc aagcgcccgt cctgaatgat attacaaatt gtggaaacag
     3961 cctaaaaatt accagccttt aacagcgccg ccgttaaaca ctttgtttgc tgcttcgtaa
     4021 acttcatcag actgataagc ctggacgaat ttcttcacgt tctcggcgtc tttgttatct
     4081 tcacgcgtta cgatcaggtt tacgtacggg gagtctttat cttcgacaaa gataccgtct
     4141 ttcgctggag tcaggccaat ctggctggca taggtggtat tgataactgc cagagcgatt
     4201 tgcgcgtcgt ccagagatcg aggcagctgc ggtgcttcca gctcaacaat tttcagattt
     4261 tttgggttct caacaacatc aagaacggtc ggcagcaggc caacgccatc tttcagtttg
     4321 atcaagccca ctttttgcag cagcagcagt gaacgaccaa gattagttgg atcgtttggc
     4381 acggcaacct gcgaaccatc ctgcagttca tccagtgatt tgattttctt ggagtaacca
     4441 gcaatcggat aaacaaaagt gttgcctact gcgaccagtt tgtagccacg atctttcagt
     4501 tgctgatcaa ggtacggttt atgctggaag gcgttggcgt cgatatcgcc tttgctcaat
     4561 gcttcgtttg gcagaacata gtcgttgaag gttaccagct caacgtccag gccatatttg
     4621 tctttcgcaa ctttctgcgc gacttctgct acctgctgtt cggcaccaac aatcacgccg
     4681 actttaatgt gatttggatc tttttcatcc tgaccgcagc ctaccagtgc cagtgatcca
     4741 atcagggctc ccactgccgc aaaggttttg aatttgaacg ccatacctta ttccttcttc
     4801 aattatttat gttgtgttga acgttacttg cgagtgacag cccggacgat gcggtcgcct
     4861 gcgaactgaa ttaaataaac cagaatgacc agcaatacca gtaccgtatt catcaccgtc
     4921 gcgttatagc cgatgtagcc atactgatag ccaatctgac ctaaaccacc ggcaccgact
     4981 gcaccaccca tcgcggaata accgaccagg gtaatcaggg tgatagttgc cgcattcacc
     5041 agacccggca atgcttccgg taacagcact ttacggacga tctgcatcgg cgtggcaccc
     5101 attgcacggg aagcttcaat taacccggtt gggatctcca gcagagcgtt ctcgaccata
     5161 cgggcaataa acggtgctgc accaacggtt aacggaacaa tcgctgcctg caaaccaatc
     5221 gatgtaccaa caataacgcg ggtaaacgga atcatccata ccagcaagat aatgaacggg
     5281 atagaacgga aaatgttcac aatcgcagaa acggtacgat acagcttcgc gttagcaata
     5341 atttgccccg gacgcgtgac ataaagcaga acgccaaccg gcagaccaat cacaaagcca
     5401 aaaaaaccgg atacgaaggt cattgccagc gtttcccata cgccacgaac cagcagccac
     5461 atcatcggct cagacataac ccagtacctc tacttttaca tggtgttcct gcagccaggc
     5521 aatggcggct tgcgtatctt gttgtgtgcc gtgcatttca gtcagcatga tgccgaactt
     5581 aacgccaccg gcgtagtcca tctgcgcgct aataatgttg ttgttgacgt tgaaacgacg
     5641 cgcggtttca gaaagcagtg gggcatcgac cgactgaccg gtaaactcca gacgcaacat
     5701 cggcacgcag tcagtaaatg gctccgcttg cagacgttcc tgataatctt ccgggatatc
     5761 cagatgcagg gtcgactgaa taaacttctg cgccagcggc gttttcggat gcgaaaacac
     5821 ttcacttacc gtgtcctgct cgatcagttc gccattgctg atgaccgcca cgcaatcaca
     5881 aatgcgcttt acaacgtcca tttcgtgggt gatcaacaga atcgtcaaac ccagacggcg
     5941 gttgatgtct ttcagcagtt cgagaataga acgtgtcgtt gccgggtcca gcgcgctggt
     6001 ggcttcatca cacagcaata ctttgggatt gctggctaat gcacgggcaa tcgccacacg
     6061 ttgtttctgc ccaccggaaa gattcgacgg gtagctatca tgcttatcgc caagaccaac
     6121 caatgacagc aattccgtca cgcgacgttt gatctcgtct ttcggtgtgt tgtccagctc
     6181 cagcggcaga gccacgttgc caaaaacagt acgcgaagag agcaggttaa aatgctggaa
     6241 aatcatacca atctggcggc gagctttggt caactcggat tctgacagcg tggtcagttc
     6301 ctggccatcg accagcacgc taccctcggt tgggcgctcc agcaggttta cacaacgtat
     6361 aagcgtactc ttacccgcgc ctgaggcacc gataacgcca taaatttgtc cagctggcac
     6421 atgcaggctg acgttgttca acgcctggat ggtgcgggtg ccctggtgga acactttggt
     6481 gatattcgaa agttttatca ttgattattt attatcgtca ttaagttagt cgtggcatct
     6541 cgaatgcctg aaacgggcaa cgccgtcaat gaaatggatg ttaaggcatc cagacgtcta
     6601 aatcaatcag gtttatgcga agagcacttt cttgcaggtc gaaacatgcg atactagcgt
     6661 cacatgcctt attaaggagc tataaaaggt ggcaaagagc gtacccgcaa tttttcttga
     6721 ccgtgatggc accattaatg tcgatcacgg ctatgtccat gagatcgaca actttgaatt
     6781 tatcgacggt gttattgacg ccatgcgcga gctaaaaaaa atgggctttg cgctggtggt
     6841 agtaaccaac cagtctggca ttgctcgcgg taaatttacc gaagcacagt ttgaaacgct
     6901 gaccgagtgg atggactggt cgctggcgga ccgagatgtc gatctggatg gtatctatta
     6961 ttgcccgcat catccgcagg gtagtgttga agagtttcgc caggtctgcg actgccgtaa
     7021 accacatccg gggatgcttt tgtcagcacg cgattatttg catattgata tggccgcttc
     7081 ttatatggtg ggcgataaat tagaagatat gcaggcagcg gctgcggcga acgtgggaac
     7141 aaaagtgctg gtgcgtacgg gtaaacctat tacgcctgaa gcagaaaacg cggcggattg
     7201 ggtgttaaat agcctggcag acctgccgca agcgataaaa aagcagcaaa aaccggcata
     7261 atgattaaaa gatgagcaat tgaaataaaa atgcattttt ccacttgtct tcctgagac
//
LOCUS       DAZGSP010000176         7294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZGSP010000176 DAZGSP010000000
VERSION     DAZGSP010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.177"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(433..918)
                     /gene="sixA"
                     /locus_tag="ACHK74_004852"
     CDS             complement(433..918)
                     /gene="sixA"
                     /locus_tag="ACHK74_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFP0223529.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(1121..3265)
                     /gene="fadJ"
                     /locus_tag="ACHK74_004853"
     CDS             complement(1121..3265)
                     /gene="fadJ"
                     /locus_tag="ACHK74_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFP0223530.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRSSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYDSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3265..4575)
                     /gene="fadI"
                     /locus_tag="ACHK74_004854"
     CDS             complement(3265..4575)
                     /gene="fadI"
                     /locus_tag="ACHK74_004854"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFP0223531.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQGTAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELHRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4755..5039)
                     /gene="yfcZ"
                     /locus_tag="ACHK74_004855"
     CDS             complement(4755..5039)
                     /gene="yfcZ"
                     /locus_tag="ACHK74_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFP0223532.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5411..6751
                     /gene="fadL"
                     /locus_tag="ACHK74_004856"
     CDS             5411..6751
                     /gene="fadL"
                     /locus_tag="ACHK74_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFP0223533.1"
                     /translation="MSQKTLFTKSALAVAVALISTQVWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            7116..>7294
                     /locus_tag="ACHK74_004857"
     CDS             7116..>7294
                     /locus_tag="ACHK74_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223534.1"
                     /translation="MLPSISINNTSATYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRV"
BASE COUNT         1902 a         1908 c         1679 g         1805 t
ORIGIN      
        1 taaggacatc cttaaaaaac acggaataag tatttagaaa aaatagaaag tatatcaata
       61 atgaatatca acagcttaaa aaacaaacaa tgaacatata atgcgattgg catttatcca
      121 cttttgacaa tataatatat aaacactata gccagaattt aatcaagttt caattacatc
      181 ctgcctgatt attcaactct attatctgta ttaataaatg acaatgagtg tcaaacagac
      241 attattcatt tcaacatgta actttgggca aaatcaagat acgcgcagaa gaataatttc
      301 cgaatgaatg tagaaagtgt tttatttata gataaggaga gtcttcgata gtagtgaggt
      361 tgacagatat ccgctgcctg atgagcaatt atgcccaatg aatctacctc attgggcata
      421 atttggaaca catcagatag cttttgccat cttcagatta cacgggctca tctgccagtt
      481 gaatgtaccg tttccgcttt catcaagcgt tacgctggca atggccgagg tggtaaacat
      541 aggcggcgtt tcgcccggac ataactcggc aaccagatac ccgactaacg gtaagtgaga
      601 gatcaccagt accgaagcaa caccttcatt ggtcaacgcc tgcaaatagg cactgaccag
      661 accaacatcg ccgcagggcg ttaactccgg cagaacttct gcactggaag gcaggttcag
      721 acaatcccct acttcttcca gtgtttgctc ggctcgcagg aacgggctca ccagaacgcg
      781 ttcgatttcc actttttgac ctttcagcca gttcgccatc aggcgagatt cgtcacaacc
      841 attagtggtc agaggacgaa ccgaatcact ggcggcatcg agggctgcgt cgccgtgacg
      901 catgataaaa acttgcatat tgcaccgctt ttgttaacca gattcgcccg ttttctttca
      961 tctgaaacca gagaaagaaa acggtggccg gcattgtgcc ttatccattc accgaatgaa
     1021 acgctgtttt ttacctcaat ggcgtaagta tagtcaatat gcgtttacat tttgaccaac
     1081 actccgccat tcagcgcgga ttcatatagc tttgaccttc ttattgcagg tcagttgcag
     1141 ttgttttcca aaaactttcc ccacgcgcgc ccatctcgac caaacgctcg caaggggtaa
     1201 aacgggaatc atactgcgtg gcaagtcgtt gcattattgc aaccacttcg cccgcgccga
     1261 gagaatcgat atagcggaac ggtccaccga gaaatggcgg aaaaccaatg ccaaataccg
     1321 cgccaatatc cccgtcacgc acgctacgga taacctgctc atcaacacaa cgtactgctt
     1381 cattcagcat caacatcaca caccgttcag caacctgcgg tgcggagagt cgcccctgcc
     1441 cttgtgcgcc aatgagcggg taaatggcag gatcgacctg ttttttgctt ttacgccctt
     1501 tctgaccata aagatagaaa ccccgaccat tttttctgcc tttgcgatcg tcgttcaaaa
     1561 ttgaagaaac aacatttgca ggcgcgctaa aacgttctcc ataagcggct tccagtacag
     1621 gaataatttt agtcccggtg tcgattccta cctcatccaa aagttggatt gggcctaccg
     1681 gaaaaccaaa tttcactagc gcagcatcaa tgtgctctac ccgttcacct tcggtcagca
     1741 tgcggatagc ttcattaatg taaggcgcta agatgcgatt gacgtaaaac ccggctttgt
     1801 cacgcacgac aatgggcgtt ttgccctgtt ttttcgccag ttttaccgta gtggcgatgg
     1861 tttgcgctga tgtacccgca tgaggaataa tctccaccag cggcattttt tccaccggac
     1921 tgaagaaatg caggccgata acttgctcag gtcgtgtggc gtgagcggcg atatcaccaa
     1981 tcggcaaaga tgacgtattt gaagcaaaga tggtatgagc ggcgcaattt tgctccactt
     2041 ccgccaccat ctgttgtttt aattcgagat tttcaaacac cgcttcaata atcagatcgc
     2101 gatgggcaaa gccgcgatag tccgtcgttc cggagattaa tgccagctgt ttgtcacgtt
     2161 cgctggcttt gagatgacga cggcgaactt tgccctccag ctgatcccaa ctgtacttca
     2221 gcgcatgatt tatgccctgc gggttgatat ctttaattct gaccggaagc cccgctttac
     2281 aagcagtgac ataagcaata ccgccgccca tcaagccacc acctaaaatt cccacgctgt
     2341 ttaatggcgc aggcggtgca tcactaccgg gatctttctt cacttccgta ctggcaaaaa
     2401 agatactacg caaagcttgc gattgtggcg tcatcgccag ttcgccaaac gcccgagctt
     2461 cggcgtcata accgctgctg gtgccctgcg ctaatcccgt ttcaacaacc tccaggatgc
     2521 gttctgtcgc cggataattg ccttgagttt tgtgttctgt tttcttgccg accattttga
     2581 acagcagcgc acgacctaac ggccccgcca gaatacgctc gcgtacaggt agagggcggg
     2641 aagatgagcg atccttcttc gccagctcaa cagcggcttc cagcaaaatg gagtgcggaa
     2701 caacgtcatc caccagcccc agctttaatg cctgtttcgc ccgaagttgt tttccggtga
     2761 gaatcatctc taatgctgta ctgacgccta tcagacgcgg taaacgctgg gtgccgcctg
     2821 aaccgggtaa caatccaagt tgtacttcag gcaaaccgag caccgtttta ggatcgtcag
     2881 tacaaacgcg accgtggcac gccagcgcca gctccagccc accgcccagg caagcgccat
     2941 gaatagccgc gataaccgga atgggcaaag catgaatctc cgccatcaac tgttggccct
     3001 gccgcgccag tgcttccgct tcttgcgccg ttttgcagtt gccgatcatg ttgatgtctg
     3061 cgccagcaat gaagttgtcc ggtttagcgg agacaaacac cacgcctcgc aactctttgt
     3121 tttcacggag ttgcttaata atggcgcgca cctgcgaggc aaactccgcc ttcagggtat
     3181 tcattttctc acccggtacg tcgatggtga taacggcaat gttgtccaga cgaacattaa
     3241 gggtaaacgc tgatgtcatt tccattattc cgcctccaga accattgccg cgccaagccc
     3301 accggcagca caggcggtaa ctaaaccaaa tccaccgccg cgacggtgaa gttcatgcaa
     3361 tgtctgggta atcatccgcg cgccggtcgc cgcgaaggga tgcccgtaag caatcgaacc
     3421 gccaagcacg ttaaatttgc tatcgtccac ttcgccagtg gcatgtgcac gccccagtac
     3481 ttcacgagca aaacgttcgc tgcccagcaa ctgaatattc gccagtgtct gagcggcaaa
     3541 ggcttcgtgc atatcgatca atgtcagatc gctcatcgtc aaaccggcac gctccagcgc
     3601 cagcggtgtt gaccaggccg gaccgagcaa catgtcctgc cagacatcaa tcgcagtaaa
     3661 tgcgtagctg cgcagatacc ccagcggcac cagccctaat tctttcgctc gggattcagt
     3721 catcaggatc accgctgccg cgccatcggt cagcggcgta ctgtttgccg ccgttaccgt
     3781 tccgtgttta cgatcaaacg ccggacgcag ctttgcgtaa tcggcaagcg aggaattacc
     3841 gcgaatattg ttgtcttcga caagcggttg tttataagga gggataaagg cagtcatcac
     3901 ctcttctttg agttttccgt ctgaccatgc ctgagcggca cgctgatgcg aacggtgcgc
     3961 taatgcatct tgctgttctc gggtgatgcc gtaggttttc gccatttgtt cggcggtgtc
     4021 gcccatccgc aagccagtag aatattctgc taccgcaggc ggtacaggca ttaagtcgcg
     4081 caaacgcagg cgagagaaga gtttcaggcg ctggctcatg gttcgagctt tgttgacatc
     4141 aaccagcacg cgcgccagtt ttttactgac gccaattggc aatacggaag aggaatctgc
     4201 cccaccggca atccccgctc ggatagttcc cgccatcagg ctttctgcga cgtttgcaac
     4261 cgcctggaaa ctggtagcgc aagcgcggct gacgctgtaa gcatcggtat gcacattcat
     4321 tccggtaccg agaacaattt cacgcgcaat gttgggggct tcaggcattt gtacgacctg
     4381 accaaagacc agctgttcaa tcacttcagc ggggatctcg ctgcgtgcca gcagttcgcc
     4441 taccaccatc ttccctaaat caaccgcggg aatgccatga aaagccgtcc cctggcgggc
     4501 aaaaggcgta cgtaaaccgc taacaatggc gatacgatcg ccctggcggg taaccagcgg
     4561 taaaacctga cccataacac tcccctgtaa aaaaataaat aaagtggtct gacctgatca
     4621 tagtcttaac cattttttta catttagcca agcggagaaa aaagaaagtg ggagctatga
     4681 cacagtgaag acaggagaaa gaaaagaaaa cgcccctgcc agcaactgac aggggcgaac
     4741 gtacccgaga ggaattaacg cagacccagc tggaagatca gcatttcagc ttcgcaggca
     4801 aaagtgaaat cgatatccag gcgcacaccg tcagactctt cagtgaaagt cggggagatt
     4861 ttgcacggtt cggattccac gctacgggct ttttcagtca gcgccgccag cgtttgttct
     4921 gcttctgcgc ggtttgcaaa cacgcggctg taagacgcgg tgcagtcgga gttgtccata
     4981 atggtgccaa catccataca gcagcaaacc ggggtttcat cagcactaca tttactcatc
     5041 gttgatttcc tctgtatgtg cacccaaggt gccagataaa cgttgtggat attttacgct
     5101 tccggaaagt gctgctccag ttgttaattc tgcaaaatcg gataagtgac cgaaatcaca
     5161 cttaaaaatg atctaaaaca aaattcaccc gaatccgcga gtgcgcaacc tccaaatttt
     5221 gccagctgga tcgcgtttct tagatcatat ttgaaaaaag atggaaacat acttgcaaca
     5281 ttccagctgg tccgacctat actctcgcca ctggtctgat ttctaagatg tacctcagac
     5341 cctacacttc gcgctcctgt tacagcacgt aacatagttt gtataaaaat aaatcattga
     5401 ggttatggtc atgagccaga aaaccctgtt tacaaagtct gctctcgcag tcgcagtggc
     5461 acttatctcc acccaggtct ggtcggcagg ctttcagtta aacgaatttt cttcctctgg
     5521 cctgggccgg gcttattcag gggaaggcgc aattgccgat gatgcaggta acgttagccg
     5581 taaccccgca ttgattacca tgtttgaccg cccgacattt tctgcgggtg cggtttatat
     5641 tgacccggat gtgaatatca gcggaacgtc tccgtctggt cgtagcctga aagccgataa
     5701 catcgcgcct acggcatggg ttccgaacat gcactttgtt gcaccgatta acgaccaatt
     5761 tggttggggc gcttctatta cctctaacta tggtctggca acagagttta atgatactta
     5821 tgcaggcggc tctgtcgggg gtacaactga ccttgaaacc atgaacctga acttaagcgg
     5881 tgcgtatcgc ttaaataatg catggagctt tggtcttggt ttcaacgccg tctacgctcg
     5941 cgcgaaaatt gaacgtttcg caggcgatct ggggcagttg gttgctggtc agattatgca
     6001 atctcctgcc gggcaaactc ctcaagggca agcattggca gctaccgcca acggtatcga
     6061 cagtaatacc aaaatcgctc atctgaacgg taaccagtgg ggctttggct ggaacgccgg
     6121 aatcctgtat gaactggata aaaataaccg ctatgcactg acctaccgtt ctgaagtgaa
     6181 aattgacttc aaaggtaact acagcagcga tcttaatcgt gcgtttaata actacggttt
     6241 gccaattcct accgcgacag gtggcgcaac gcaatcgggt tatctgacgc tgaacctgcc
     6301 tgaaatgtgg gaagtgtcag gttataaccg tgttgatcca cagtgggcga ttcactatag
     6361 cctggcttac accagctgga gtcagttcca gcagctgaaa gcgacctcaa ccagtggcga
     6421 cacgctgttc cagaaacatg aaggctttaa agatgcttac cgcatcgcgt tgggtaccac
     6481 ttattactac gatgataact ggaccttccg taccggtatc gcctttgatg acagcccagt
     6541 tccggctcag aatcgttcta tctccattcc ggaccaggac cgtttctggc tgagtgcagg
     6601 tacgacttac gcatttaata aagatgcttc agtcgacgtt ggtgtttctt atatgcacgg
     6661 tcagagcgtg aaaattaacg aaggcccata ccagttcgag tctgaaggta aagcctggct
     6721 gttcggtact aactttaact acgcgttctg ataacgcgtt cgcctggata aagtcacctg
     6781 catagcaggt gactttaact cccccacttc accgaaagta gtgtcctcat tgcttacctc
     6841 ctgagttttg caaacagcct gttggcagct tgccctttca aatcaataag cggtatccac
     6901 agacaaacct gaagggaaag gcatattttc aggcgttctg ctcgtccttc tcaaagagtt
     6961 tacttttctg catttccagg atactccccc ccctggctat tgtgcgctca tacactcaaa
     7021 ttaaagatag gttctaaata aatgagcgtt ttttgatagt ctatttcatt aggtaatata
     7081 tatttgtaac aaatcaatca aaatggaata aaatcatgct accatctatt tcaatcaaca
     7141 ataccagcgc aacttaccca gaatccatta atgaaaataa caatgatgaa attaatggat
     7201 tagtacaaga gttcaaaaac ctttttaatg gtaaggaagg aataagcacc tgtattaaac
     7261 atctacttga gcttataaaa aacgccatac gagt
//
LOCUS       DAZGSP010000177         7276 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZGSP010000177 DAZGSP010000000
VERSION     DAZGSP010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.178"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1353
                     /gene="paoC"
                     /locus_tag="ACHK74_004858"
     CDS             <1..1353
                     /gene="paoC"
                     /locus_tag="ACHK74_004858"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFP0223535.1"
                     /translation="PATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELL
                     YAGANRHTGLRLATLDLPEGNAMRASGEAPGLMALEIAIDELAEKAGIDPVEFRILND
                     TQVDPADPTRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNN
                     LLEKSGARVHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSF
                     PVSAGSGGQWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLH
                     EATAGGRLTAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRI
                     LNPKTARSQVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIF
                     LDDTDPISSPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV
                     "
     gene            1363..2319
                     /gene="paoD"
                     /locus_tag="ACHK74_004859"
     CDS             1363..2319
                     /gene="paoD"
                     /locus_tag="ACHK74_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFP0223536.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIHGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPALQAAREAKPFYLGALGSHRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEIASVRLHHEEDSCLPPSS"
     gene            2370..2708
                     /locus_tag="ACHK74_004860"
                     /pseudo
     CDS             2370..2708
                     /locus_tag="ACHK74_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(3029..3397)
                     /locus_tag="ACHK74_004861"
     CDS             complement(3029..3397)
                     /locus_tag="ACHK74_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP0223537.1"
                     /translation="MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFS
                     DETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGFTSQVQAPYLTATDILQARKACG
                     LMSRCSYRDVSNIVLSRSRL"
     gene            complement(3418..3776)
                     /locus_tag="ACHK74_004862"
                     /pseudo
     CDS             complement(3418..3776)
                     /locus_tag="ACHK74_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
     gene            complement(3827..4471)
                     /locus_tag="ACHK74_004863"
     CDS             complement(3827..4471)
                     /locus_tag="ACHK74_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HFP0223538.1"
                     /translation="MSRIITTTVYTLHELSSTAQEKARDWYRQHHADSNWYENVYEDF
                     REVCGIFGIDLRQRVFRLSNGRFMEEPCIWFSGFCGQGDGACFEGRWHWQPAAPRKIR
                     EYAPQNRELHRISDALQVVQKRNFWQLQTEIHHRGRDCHPYSMDITVTRNSPTGQAMT
                     TDAEAAVSEALRDLAFWLYRQLENEYDWLTSDAAVDEALLINEYTFTEAGLRAG"
     gene            complement(4490..4711)
                     /locus_tag="ACHK74_004864"
     CDS             complement(4490..4711)
                     /locus_tag="ACHK74_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP0223539.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAGAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(4780..5256)
                     /gene="radC"
                     /locus_tag="ACHK74_004865"
     CDS             complement(4780..5256)
                     /gene="radC"
                     /locus_tag="ACHK74_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP0223540.1"
                     /translation="MQQLSFLPGEMTPGERSLIHRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(5272..5745)
                     /locus_tag="ACHK74_004866"
     CDS             complement(5272..5745)
                     /locus_tag="ACHK74_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0223541.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPESAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(5839..6084)
                     /locus_tag="ACHK74_004867"
     CDS             complement(5839..6084)
                     /locus_tag="ACHK74_004867"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223542.1"
                     /translation="MKHIPEGVIQVPESPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     EISLCRLSTGNGIWPSVCDRNGYAYGCPEFQPNIIHP"
     gene            complement(6084..6902)
                     /locus_tag="ACHK74_004868"
     CDS             complement(6084..6902)
                     /locus_tag="ACHK74_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP0223543.1"
                     /translation="MRLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRDGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(7081..7212)
                     /locus_tag="ACHK74_004869"
     CDS             complement(7081..7212)
                     /locus_tag="ACHK74_004869"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFP0223544.1"
                     /translation="MAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIRNAEGQRR"
BASE COUNT         1623 a         1958 c         2050 g         1645 t
ORIGIN      
        1 cccgccaccc ttcagcactt gcgtatcggt gccgaccaga gcgggaaaat caccgctatc
       61 tcacatgaaa gctggtctgg aaacctgccc ggcggcacgc cggaaacggc ggtacagcaa
      121 agcgaattac tctacgccgg ggcgaatcgt cataccggcc tgcggctcgc cacgcttgat
      181 ttgccggaag ggaacgccat gcgtgcgtcc ggcgaagccc ccggtctgat ggcgctcgaa
      241 atcgcgatcg acgaactggc ggaaaaagcg ggcatcgatc ccgtcgagtt tcgcatcctg
      301 aatgacactc aggttgaccc cgccgacccg acgcgctgct tctctcgccg tcagcttatc
      361 gagtgcttgc gcaccggagc ggataaattt ggctggaagc agcgcaacgc cacacccgga
      421 caggtgcgcg acggggagtg gctagtcggc cacggtgttg cggcgggctt tcgcaataat
      481 ctgctggaaa aatcgggtgc tcgggttcac ctcgaacaaa acggcaccgt taccgtagaa
      541 acggacatga ccgacattgg caccggcagc tacaccattc tggcccagac ggcagcggaa
      601 atgcttggcg taccgctgga gcaggttgcg gttcacctcg gcgattccag tttcccggtt
      661 tctgcgggtt ctggtggaca atggggcgcg aatacctcca cctccggcgt ttacgccgcc
      721 tgtatgaagc ttcgcgaaat gattgcctcg gcagtcgggt ttgatcctga gcagtcgcag
      781 tttgccgacg gcaagattac caacggtacc cgaagcgcca cgctacatga agccaccgca
      841 ggcggcagac tgacagcgga agagagcatt gaattcggaa cactgagcaa agagtaccag
      901 cagtcgacct ttgccgggca ttttgtggag gtcggcgtgc atagcgcgac gggagaagtt
      961 cgggtccggc gtatgctcgc tgtgtgtgct gcaggacgca tcctgaatcc gaaaactgcg
     1021 cgcagccagg tcattggcgc aatgactatg ggcatgggcg cggcactgat ggaggagctg
     1081 gcggtggatg accgtttggg ctacttcgtt aatcacgata tggcggggta tgaggtgccg
     1141 gtccatgcgg atatcccaaa acaggaggtg attttcctgg atgataccga ccccatatcc
     1201 tccccgatga aggccaaagg tgtcggtgag ttgggcctgt gcggcgtgag cgcggctatc
     1261 gccaacgcgg tgtataacgc caccggtatt cgggtacggg attatcccat cactctggat
     1321 aagctgctcg ataagctgcc ggatgtggtt taaggagaaa caatgtcata cccgcttttt
     1381 gataaagacg aacactggca taagccagag caggcgtttc tcaccgatga ccaccggacc
     1441 attctgcgct tcgccgtaga ggcgctaatg tccggtaaag gagcggtgct ggtgacgctg
     1501 gtggagatac acggcggcgc ggcgcgcccg ctcggggcgc agatggtggt tcgcgaagat
     1561 ggtcgttact gcggttttgt ctctggcggc tgcgtggagg ccgctgccgc ttttgaagcg
     1621 ctggagatga tgggctcagg ccgcgatcgc gaaattcgct atggcgaagg ttcgccgtgg
     1681 tttgacatcg ttctgccctg cggcggtggg atcacgctta cgctccataa actacgctcg
     1741 gcacagcctc tgctcgccgt gctgaaccgt ctggaacaga gaaaaccggc ggggctgcgc
     1801 tacgatccgc aagcacaatc gttggtgtgc ctgcccacgc aaacccgaac gggctggaat
     1861 ctcaatggct ttgaggtggg gttcaggcca tgcgtcaggc tgatgattta cggacgttct
     1921 cttgaggcgc aggcaaccgc gagtcttgca gcagccacag gctatgacag ccatatcttc
     1981 gatctttttc cggcctcagc cagcgctcag atcgataccg acacggcggt cattttgctg
     2041 tgccatgatc tcaaccggga gctgccagca ttgcaggccg cgcgagaagc aaaacccttt
     2101 tatctcggcg cattgggcag ccatcgaacc cacacgttac gtctgcaaaa gctccacgag
     2161 ctgggatggt ccagggagga gacagcgcaa atccgggccc ccgtcgggat attccccaaa
     2221 gcccgggatg cgcatactct ggcactctcc gtgctggcag aaatcgcctc tgtacgtctc
     2281 catcatgagg aggattcatg cctgcccccg tcgtcctgat ccttgcggcc gggcgtggag
     2341 agcgctttct cgcctccggg ggaaataccc ataagtgtat cggctggcgt cagtccccgg
     2401 aggttgcgcc ttatcgctgg ccatttgaag aaaacgggag aactttcgac cttgcgattg
     2461 aaccgcagat tacgactaat gatctgcgtc tgatgttgag gctggctctt gccggcggag
     2521 gaataacaat tgccactcag gaaactttca ggccatatat tgaaagcggt aagcttgtat
     2581 cgctgcttga tgactttctt ccacaatttc cgggcttcta tctgtatttc ccacagcgtc
     2641 gcaatattgc accaaagctc cgcgccctga ttgactacgt caaagaatgg cggcagcaat
     2701 tggtttaaat agctgcacct gcactgcctg atgtcagaac agtattttga tgaattgcca
     2761 aggttacaat ggcacaaatg cggcacagga ggaaacgtgg tgtatttaaa tatggggtaa
     2821 ctctttgatt ttaatggtgc gataatagga acaaaacaca tcacatatac ctattgataa
     2881 atataactat tttcttattt catctttata aatgccccct tttatgcccc caccttgttt
     2941 ttcatgcgac tcgatgagac tttcatcgag tcgttaatct catcaaattc ttcacgtatc
     3001 gtggatacct tcgcagatct ccatcccctc acaaccgtga tcggctaaga acaatattac
     3061 ttacatctct gtaagaacac cggctcatca acccgcacgc tttcctggct tgaagaatgt
     3121 ctgtcgcagt caaatatggt gcttgtacct gagaggtaaa tccattgcga tcaatacgaa
     3181 ctaactcata tttctctacc agaaagttaa cggcattagc cagagtgata ccggcatcaa
     3241 tatgctcctt tataacggtt tcatcgctaa aaggtgtgtc gcttagcatt aggccatagt
     3301 gctgctcaag caaacgagta agtaacattt gccagactgc aacgggcgac ggacagggct
     3361 tcgccgccgg agaaattgta gcaggtagag tgttcatgct tgctctcgtg aaagtaatta
     3421 gagctgagtg ggataaatgg cgatgtatac atagccgcaa ctaccaaggg tatcggcttc
     3481 gcaggtgaaa ccgttgtggt acagggtgaa acagtgggca tggcggggat tcatttcacc
     3541 agtggtcagc atcgattcca tctgactgat aaaatgtgga aatacgacat ccagcttcag
     3601 gctttcggca tcactaaact taccggtgat acttgcccgg tcagccaaat agtacagtcg
     3661 gttgccctcc tgcaccagac gggtgccaga acagggcgtc acggtgtagg gtagccccac
     3721 caggggcggt cgttgttgtc gtcgggatgc gttgtcccgg agagtgtgtc tggcacgata
     3781 aaatcctcac aaaaaatcgg ttaagaacgg ttttaacaat gacaggtcag ccggcgcgta
     3841 gaccggcttc ggtaaaggtg tattcgttga tgagcagggc ctcatccaca gcggcatctg
     3901 aggtcagcca gtcgtactcg ttttccagtt gacggtacag ccagaaggcc aggtcgcgca
     3961 gggcttcgct gactgcggct tccgcatcgg ttgtcattgc ctgaccagtc ggactgttcc
     4021 gggtaaccgt gatatccatg ctgtacggat ggcagtcgcg tccccggtgg tggatttcag
     4081 tctgcagttg ccagaagtta cgtttctgca ccacctgcag ggcatccgag atacgatgaa
     4141 gctcacggtt ctgcggggca tattccctga ttttccgggg tgccgctggc tgccagtgcc
     4201 agcgtccttc aaaacaggct ccgtccccct ggccgcagaa tccgctgaac cagatgcacg
     4261 gctcttccat aaacctgccg ttgctgagcc ggaaaacgcg ctggcggagg tcaattccga
     4321 aaatgccaca gacctccctg aagtcttcat acacgttttc ataccagttg ctgtcagcat
     4381 gatgctgacg gtaccagtcg cgggcttttt cctgtgcggt ggaggatagc tcatgcagtg
     4441 tatacacggt ggtggtgata attctgctca tcagaatgcc cttactgact cagttcagag
     4501 tgacgctcat cagccggaca tacgggccaa aactgtcctt acggcgttca gcaaacacgg
     4561 ccagcacacc gggaatatcc tgcacttcac gaccggtata cgccccggca ctgccgtgcc
     4621 agcggtactt accggtgcag aacggaaaaa gacgggatgc cggatgctgt tggtgaatac
     4681 gcatggcttc accacgggtg atgattttca tgatgtgata cctctgaaga cagaagataa
     4741 aagtgaaaac aggtatgatg tggttgtgac ggtgacgggt taaagcagac cgtgttcggc
     4801 aaaggagaaa acctggctgc caccgactat cagatggtcc ggcacccgga tatccaccag
     4861 ggccagtgcc tgtaccagac gttcagtgat aagccggtca gccttactgg gtgtgacttc
     4921 gccggacggg tgattgtgtg ccagtaccac ggcagcggca ttgtggtaca gggcgcgttt
     4981 aatcacttcc cggggatgga cttccgtgcg gttgatggtg ccggtgaaga gggtttcacc
     5041 ggcaatcagc tgattctggt tgttcagata cagcacccgg aattcttcac gctccagtcc
     5101 tgccatgttc agaatcagcc attcccgtgc cgcacgggtg gaggtgaagg ccacgccggg
     5161 ttcatgaaga tggcggtcca gggttttcag ggcccgatga atgaggctgc gctcgccggg
     5221 cgtcatctct ccgggcagaa aggaaagctg ctgcattgtc cctgcctcca ttcagtcgat
     5281 gatgcgcata atggcgctgc attccggatg ctgcagggcg taatcccgca gccggtaata
     5341 atggaccgtc atggcataat tctccgtacg acaggcatga tgactgtacg tcatcagaca
     5401 ggcggcaata ccggcggatt ccgggctcat ttcagcgcgg ttaccgttca tggcattgaa
     5461 cagcacccat gtttcgtcat catcgtcatc cggttcgggt gccataaatg ccccgccatt
     5521 gttcagggta tacagattcc agataccacc gcagtagtct tcacacagac ggtccatcca
     5581 gccgaagata cggggctcca gggtcaccca ctgtggaatg aggccaaaat gctgcggcca
     5641 gaaactgatg cgctgttcat cagggactat ggtggcaacc agttgttgca ggccagtctc
     5701 cggtgcacag gccgaagagg tagtgttctg agacaatgtt ttcatgtatg aaatccttgt
     5761 aatgtgaata aatgagaggg agtaaaagtc gggagctggc aacatcacgt tcatcactga
     5821 ccgtgagacc cggtaatgtc agggatgaat gatattcggc tgaaattccg gacagccgta
     5881 tgcgtaaccg ttccggtcac agacggatgg ccagatgcca ttgcctgtac tcagtctgca
     5941 cagactgatt tctccgggac ggtaaagcag acgggtacac caggcgcaca ggtcatcatc
     6001 agaaatggaa cgacaccccg gcagcgcttc cggggcgtcg ggggattctg gtacctgaat
     6061 cacgccttca gggatatgtt tcatcagcgc atactctcca gcagcgtttc tgccatcacc
     6121 cacaatgcgc ggttgagctt aatatcggtg tcgatgctgt gaatggcacg ggtgtggata
     6181 cgttttcctt ttgcactgcg accggaaatc ccgcctttca gcatattctc ctggatggtc
     6241 tgataagcac tccacaggtc cttaccgtaa tcctcccggc gtcgtggcgt cagaatgtcg
     6301 gcggtggtga cgggctgatg ttcgtcacca taacggtaag tcagtgccgc ctgtgccagc
     6361 gcctggcgtg ccggtggcgg caggaccagc gactgcatgg catcacgttt ctcttcaatc
     6421 cggtcaaaaa cccccaccac ctcgtaagcc ccttcaatga ctctgtccac cacatttccc
     6481 cggtgcggaa cacgcacttc ccccagagac tgaccacaga cgcatccgtt ctggcagacg
     6541 aaccggaagt aacccggcag catctggtag ctggaggtac cgtcatggga gttgagcaga
     6601 atgatttcag ggacatgttc tccgtttatt tccccgtcac gccgcagacg cagcatgtgt
     6661 tttgtgtatc cccggcggcc cgggtcgcgc acacgggtct ggcaggcgaa aaagggctgg
     6721 aagccttccc gctgcagact ttccagtacg gtgatggtag ggatgtatgt atagttctgg
     6781 cttcgggacg tgtgtttatc ttctccgaaa atccccggca catggtgcat cagctcttca
     6841 tgtgtcagcg gacggtcacg gcgtatctgg ttcgcataac caaaacgact gactaatcgc
     6901 atactttgct ccttatcggt ggttaagatt tactggtgca ataaatgaaa aaagccgcgt
     6961 ctcccggaga agacgcggcc tgatggatga aatcaatgag gtttattgtc tgagaagccc
     7021 tccactggcg agccacgcat taagctgttt tccggcatca gcttcagagt tccggcagcg
     7081 tcagcgtcgc tgaccttccg cgttgcggat aacccgacgg aaatgcattc ccgggtcatc
     7141 ctggataaac acattgcggt aagcggttat gacagcaacc gcctgcccgt atgagaaaga
     7201 tccttcagcc aggacatact ctgtgtgtaa cccggcatat ctggtttctc ctgataaata
     7261 gcctctgcca caacgt
//
LOCUS       DAZGSP010000178         7167 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZGSP010000178 DAZGSP010000000
VERSION     DAZGSP010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.179"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            115..1449
                     /gene="adeQ"
                     /locus_tag="ACHK74_004870"
                     /gene_synonym="yicO"
     CDS             115..1449
                     /gene="adeQ"
                     /locus_tag="ACHK74_004870"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFP0223545.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1502..1954
                     /gene="yicN"
                     /locus_tag="ACHK74_004871"
     CDS             1502..1954
                     /gene="yicN"
                     /locus_tag="ACHK74_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFP0223546.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2165..3355
                     /gene="nepI"
                     /locus_tag="ACHK74_004872"
     CDS             2165..3355
                     /gene="nepI"
                     /locus_tag="ACHK74_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFP0223547.1"
                     /translation="MSEFIAENRGADAINRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3396..3689)
                     /gene="yicS"
                     /locus_tag="ACHK74_004873"
     CDS             complement(3396..3689)
                     /gene="yicS"
                     /locus_tag="ACHK74_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFP0223548.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3911..4729
                     /gene="nlpA"
                     /locus_tag="ACHK74_004874"
     CDS             3911..4729
                     /gene="nlpA"
                     /locus_tag="ACHK74_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFP0223549.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKITTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4733..5656)
                     /gene="yicL"
                     /locus_tag="ACHK74_004875"
     CDS             complement(4733..5656)
                     /gene="yicL"
                     /locus_tag="ACHK74_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFP0223550.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5767..6951)
                     /gene="setC"
                     /locus_tag="ACHK74_004876"
     CDS             complement(5767..6951)
                     /gene="setC"
                     /locus_tag="ACHK74_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFP0223551.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSSHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         1811 a         1605 c         1800 g         1951 t
ORIGIN      
        1 attattaaag aatatccatt aatgtgcaat tgaaatgtga taattatcac tgaaccgttg
       61 ttgtcatttg ttttacaaaa gcaagggatt aaatctcctc caatggacaa aaaaatgaat
      121 aatgacaata ccgattacgt gagtaatgaa tcagggacgc tttcgcgatt atttaaacta
      181 cctcagcatg ggaccaccgt ccgcacagaa ttgattgcgg ggatgaccac ttttttaacc
      241 atggtgtaca tcgtttttgt gaacccgcaa atcctcggcg cggcacaaat ggacccgaaa
      301 gtggtgtttg ttaccacctg tttgattgcc ggtatcggca gtattgcgat ggggatattt
      361 gctaacttac ccgtggcgct ggctccggca atggggctga acgccttctt tgccttcgtg
      421 gtcgtggggg cgatgggcat ctcctggcag accgggatgg gcgcaatatt ctggggcgca
      481 gttggactat ttttgctcac gctgtttcgt atccggtact ggatgatctc caacattccc
      541 ttaagtttac gtattggtat caccagcgga attggattat ttatcgcctt aatgggatta
      601 aaaaatactg gcgttattgt cgccaataaa gacacgctgg tgatgattgg cgatttaagt
      661 tctcacggcg tgttgttagg tattttaggg ttttttatta taaccgtgtt gtcatcacgt
      721 cattttcatg ccgcggtgct ggtttctatt gtggtgacgt cttgctgtgg attatttttc
      781 ggtgatgttc attttagcgg cgtctattcc attccgcctg atattagcgg cgtcattggt
      841 gaagtagatt tgagcggcgc gttaacactt gaactcgccg gtatcatttt ctcctttatg
      901 ctgatcaacc tatttgattc atcaggaaca ttaattggtg taactgataa agcgggctta
      961 atagatagta acggtaaatt ccccaatatg aataaggcgc tgtatgttga tagcgtcagt
     1021 tcggtggcgg gggcgtttat cggcacctcg tctgttactg cctatattga aagtacttct
     1081 ggtgtggcag tcggtggtcg cacggggctg actgcggttg tggttggcgt tatgttcctg
     1141 ttagttatgt tcttctcacc gctggtggcg atggttcctc cttacgcaac cgccggagcg
     1201 ttaatctttg ttggcgtgct gatgacttcg agcctggcgc gcgttaactg ggatgatttt
     1261 accgaatcgg tgcctgcgtt tattaccacg gtgatgatgc cctttacttt ctcgatcacc
     1321 gaagggattg cactcggctt tatgtcgtac tgcatcatga aagtgtgcac cgggcgctgg
     1381 cgcgatctga acctgtgtgt ggtggtggtc gcagctctgt ttgcactgaa gattattctg
     1441 gtggattagt aactggcgag ggtttccaga tatcatgagt tctgattacg caggagaact
     1501 catgatctgg ataatgctcg ccacgctggc ggtagtgttt gtggttggtt ttcgggtgct
     1561 gacatccggg gccagaaaag cgattcgccg tctcagtgat cggctgaata tcgatgtcgt
     1621 acccgtggag tcgatggtcg atcaaatggg aaagtcagcc ggtgacgaat ttttacgtta
     1681 tttgcatcgc ccggatgagt cgcacctgca aaacgccgcg caggtgttgc tcatctggca
     1741 aattgtcatt gtcgatggta gcgaacagaa cctgctgcaa tggcatcgga ttttacaaaa
     1801 agctcgcctc gccgcgccga ttaccgacgc tcaggtcagg ctggcgctgg gttttctgcg
     1861 cgaaaccgaa cctgaaatgc aggatattaa tgcttttcag atgcgctata acgcgttctt
     1921 tcagcctgcc gagggcgttc actggttgca ttgaaaccgt ttcgtcttat cagcctacag
     1981 accgcgcctg acctgtcggg tggataagac gttcacgccg catccggtaa tctttgccct
     2041 gttccgtttc gatcttaact ccagctcaag caacaccgta aaagttgcaa aaacgttaaa
     2101 tgcgtcacac atttcaatgt taaagtggtc ggcttttccc ctgaaacatg ccacgggtaa
     2161 caccatgagt gaatttattg ccgaaaaccg cggcgcggat gccatcaacc gaccgaactg
     2221 gtcagccgtt ttctcggtgg cgttttgtgt cgcctgtctg attatcgttg agtttttgcc
     2281 cgtcagtttg ttgacgccaa tggcccagga tttaggcatt tcggaagggg ttgccgggca
     2341 atcggtgacc gtgaccgcct ttgtggcaat gtttgccagt ttgtttatta cccagacaat
     2401 tcaggccact gaccgccgct acgttgttat tttgtttgcc gttttgctga cgctctcctg
     2461 cttgctggtt tcctttgcta actcattcag tttgctttta atcggtcgtg cctgtctggg
     2521 gctggcgctg ggcgggttct gggcgatgtc ggcgtcgctg accatgcgtc tggtgccgcc
     2581 gcgtacggtg ccgaaggcgc tgtcggtgat cttcggcgcg gtttctattg cgctggtgat
     2641 tgccgcgccg ttgggcagtt ttttaggcga gcttatcggt tggcgcaatg tctttaatgc
     2701 ggcggcggtg atgggcgtgc tgtgtatttt ctggattatc aaatcattgc cttcactgcc
     2761 aggcgaaccc tcgcatcaga aacaaaatac tttccgctta ttacaacgtc cgggtgtgat
     2821 ggcagggatg atcgccatct tcatgtcttt cgccgggcag tttgctttct tcacatatat
     2881 tcgcccggtg tatatgaacc tggcgggatt cagcgtggat ggcttaacgc tggtgctgtt
     2941 gagttttggc attgccagct ttatcggtac gtcgctttcg tcattcattc ttaaacgttc
     3001 ggtaaaactg gccttagcag gcgcgccgtt aatactggct gtgagtgcgt tggtgctgac
     3061 gttgtgggga agcgataaaa tcgttgctac cggcgtggcg attatctggg ggctaacttt
     3121 tgcattggtt cccgtcggct ggtcaacgtg gatcacccgc tcgctggccg atcaggcaga
     3181 aaaagccggg tctattcagg tggcggttat tcagcttgct aatacctgtg gcgcggcaat
     3241 cggcggttat gcgctggata atattggtct gacttcgccg ttgatgttgt ccggcacatt
     3301 gatgttgctg actgcattgt tggttactgc aaaggtgaaa atgaagaaat cctgatatca
     3361 ggtaaagaag cgttggtcag tagttgttgc tgttcttaca tatccgggca ttctacctta
     3421 cccaacgctt cccaataatt gctctgatta ttacgttcca tcgccacgat ggcctcgcgt
     3481 atatgttgtt tattgttttc atctgacaac attaactttt cccacgcttc atctgataat
     3541 gacgcctgtg gcgtgcaaat tttacgtaaa tcttgtaata ccgtggtttt ctcttttgcg
     3601 gactgcagtg agctaaaagg cgactcggcg tatacgatgc ctggcatagc gaaaaaagtg
     3661 aaaataagca gtagtgtcgt tggtttcatg gattcaacct tttacaaatg tgtatcgaat
     3721 gaagcataag tgtagtttgc tttcatcgct ctcatcatgc tgtacgaaac gcgcttgcgt
     3781 ggtcagtaag aagtgccaga ctttatattc cacttttatt cctttttatt cttatcgata
     3841 gcgtttcgtt ttttaaaccg cagcgacctt accgctatag tcaggtaatc attaataaaa
     3901 ggataaaaaa atgaaactga caacacatca tctacggaca ggggccgcat tattgctggc
     3961 cggaattctg ctggcaggtt gcgaccagag tagcagcgat gcaaaacaca ttaaagttgg
     4021 cgttataaat ggcgcagaac aagatgtcgc ggaagtcgct aaaaaggtgg caaaagagaa
     4081 atatggcctc gatgttgaac tggtaggatt tagcggttcg ttgctaccga acgatgcgac
     4141 aaatcatggc gaacttgatg cgaatgtctt ccagcatcga ccctttcttg aacaagataa
     4201 tcaggcgcat ggttataaac tggtagcggt gggaaatacg tttgtcttcc ctatggcggg
     4261 ttattccaaa aaaatcacaa ccgtggcgca aataaaagaa ggcgcgacag tggcgatccc
     4321 caacgacccg accaaccttg gtcgcgcact tttactgttg caaaaagaga agctaattac
     4381 tctgaaagag ggaaaagggt tattacctac cgcgctggat ataaccgata atcctcgtca
     4441 tttgcagatt atggaactcg agggggcgca gcttccgcgc gtactggatg atcccaaagt
     4501 tgatgtagcg attatcagta ccacttacat tcagcagaca gggctttctc cggtgcacga
     4561 cagcgtattt attgaagata agaattcgcc gtatgtgaat attttggtgg cacgggaaga
     4621 taataagaat gcagaaaacg tgaaggaatt tctgcaatct tatcaatcac ccgaagtcgc
     4681 taaagcggca gaaaccattt ttaacggtgg cgcggtgcct ggctggtaat tctcactcat
     4741 gccgcgccgg acgatttatt tttctggcgc ggcgacggga atccattgaa atcaaaatca
     4801 ccgatgacag aatcagcagc gttcccagcc agtccggtaa tgtgaacgtg atccccagca
     4861 acagcaaaga gagtagcgcg ctacttaatg gttctgcaca gctcaaaatg ctggcttttg
     4921 gaccgccaat taattgtgct cctttcaggt acagactaaa tgtcagggac gtaccaatga
     4981 ccaccaaata aaaaaacgcc agaatcaaac tgccgttaac gacaaagttt gttccttgtc
     5041 tggcataaaa aggcaacaga atcagaccgc caatcagcat actccagccg acgactggta
     5101 acgtgccata gcgggcaatt agcgttgagg gataggtggt atagaatgca gcagcaaatg
     5161 ccgaggcaat gccccagaac aacgcggcag gagagatcga taatgacgtc ggattaccgt
     5221 gtgtcaccaa taaaaaagtc ccgaccagcg atgtcaaaat agcgcagaaa accagaatgc
     5281 ccgggcgcga tttacgcacc agtgagaacc aggcgacgat aatcgtcggt gagaggaatt
     5341 gcagcaccgt tgccgtggct gcgttcgatt tttcgatggt tagcaaaaaa gtgagctgta
     5401 cagttagcgc gccaaccacg gaaaaaatca gcaggctaat ggcatcttta tgattgttaa
     5461 taatagaaaa gattttatcg ccatgaacaa atgacagcgt cagtagaatc aaaccggcga
     5521 atatcaaacg cgtcatagtc aaaaactgcg acgacatctg gctttgctcc atgatgtatt
     5581 gcgcgcagac ccctgaactt ccccacaaca cggcggcaat cagaacgttc agcatcccct
     5641 ttctggtgga acccattttt ccctctgcat actgttttgt cgtttatgta tgcaggcatg
     5701 atagcaaaaa tggcgaggat ggctctgttt gcgccacgcc gatttttctt agcccagact
     5761 ggtgagctaa atatctttaa taaacagcag gcaaatcatc gcaataccca acatccctat
     5821 cgccagccag aacaacgcat ggtagctcca gatttcaacc ataattccgt caacagagcc
     5881 ggcgataatc cagccgacgc gtgaagtatt tgcatataac gttgtcgcag agcctatttt
     5941 ttcaggcatc aagtcctgaa aataaagcat gccgatacca cagagaatac caaggaagat
     6001 ggcattaaga atttgcaatt ccagctcaac cgccggagtc gtcgccatga gtacgctggc
     6061 gtaaaaacac attccactca cgatagcaat gagcattaat agtcgcttgc caatacgttt
     6121 catgtaatag cctgcgatta acatcatcgg aatttccaga ccggcagcga taccaatcat
     6181 ttctccagcc agtttatcgg ttagatgcag ttcatcaata ataaatagcg gcatatttat
     6241 catgtagaga ttattcgccg cccacatcat tgaacagacc acaaaaagta gccgcgtatc
     6301 ccgcttcctg tgggaggagg gtaaaatttc tacgggttgg gtaacgacag gaatatttct
     6361 ttgtattgat ggcaaaaaca accagactat cagtccgcaa acaacaaatg cgatggcagc
     6421 ggtgagatac atcactttaa aactaaatcc cattgccagt tcataagcga gcggtggccc
     6481 gataacccag gcaagcgaga tctgagcacg taaaaatgta ctgaacatga ccgtctcacg
     6541 gcctgttctg tcggcgtgtt cacgggcgag ggcgaacatt tgcgggtttg cggtggaagc
     6601 aaaactactc agaagtacgc ccgttgagag gagaatgaag tagttgcgat tccacgcaaa
     6661 aagcgtgcag gccagcactc caaataagca acatagcaga atcagtaatt tacggtcgcc
     6721 ttgtttatcg gagtgccttg ccagaaattg actgaccaga attcccataa tagcgctacc
     6781 ggtgaagaaa aaacctacca ttataggacg ggctttcagt tcatctgcga ggaatatact
     6841 tagggtagga gtctgaagag cgcccgcaat acccgtcaga aaggcgacaa gtaaaaatgc
     6901 cgcggcagtg agatcaagta tttttgatgg agtggtagcc gttttttgca ttcttctgaa
     6961 tcaccgggcg aaaaaatata actgagttac aaataagtac cagtcgaatg agtattgtat
     7021 taagaatttt tgagtatcgc tgatacaacc agaaagcaat gctagcgcaa tattataggg
     7081 ccagcaattg agattattag caaaagcatg ttaagaatta attaccgaat ttatatatta
     7141 attatagtaa ggcaatgagc tattttt
//
LOCUS       DAZGSP010000179         7053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZGSP010000179 DAZGSP010000000
VERSION     DAZGSP010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.180"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..125
                     /gene="mchI"
                     /locus_tag="ACHK74_004877"
     CDS             <1..125
                     /gene="mchI"
                     /locus_tag="ACHK74_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="microcin H47 immunity protein MchI"
                     /protein_id="HFP0223552.1"
                     /translation="VGEGNSYVDVFLSFIIFLGFIELIHGIRRILVWSGWKNGS"
     gene            164..391
                     /gene="mchB"
                     /locus_tag="ACHK74_004878"
     CDS             164..391
                     /gene="mchB"
                     /locus_tag="ACHK74_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47"
                     /protein_id="HFP0223553.1"
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALAGIPGGPLG
                     VVVGAVSAGLTTAIGSTVGSGSASSSAGGGS"
     gene            663..2213
                     /locus_tag="ACHK74_004879"
     CDS             663..2213
                     /locus_tag="ACHK74_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
                     /protein_id="HFP0223554.1"
                     /translation="MSHQCSLSELNENLVPFTARQIKASLIWCAEDVRNPDELQNACS
                     YIIDPGSTASAKVFHAERYGGSGIQRNGGGARCGFDGNYQVKGIGSNPLVGEGTDERH
                     SNGALGAVHAIYEALWGEVLAQILPYSAVRVRAVLLTDLYTEKAFERSGRKSRRALLV
                     REPVVRPAHFERAPYFQVKPEYSSLLIHDACRVRSVIHKLPGYLPVPPEEIDAEARTD
                     PRIYCIEGLCELARREAWQMAFCRTRFLRLTTSPSNIAMDGRLMDFNGLSCLFPGDSP
                     ADFGYKLRLAELAKEPMVLMQGLSDLCLYIGKYMFDPDFTLAARLKVEEIFQKTFHEA
                     CYYCYLELLGIPGEFITQKEIPDILKELVNSFVALLNQYCEKSHAQDIVNQDGSPLQK
                     LVVTLIHHRHNQKQALNSSIKNDVHFTVAQQCFSQAIHWLTQGSTRRQINASLLLKEI
                     EHHTMKRLQPREELRKENMCEKIAILLDNHGDDPLFLQEAISDMKNFMLKFSRDAFGY
                     LEPIRNTV"
     gene            2239..2691
                     /locus_tag="ACHK74_004880"
     CDS             2239..2691
                     /locus_tag="ACHK74_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFP0223555.1"
                     /translation="MSYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFLFDSRGFPLAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWI
                     LDFCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTYIPERLHKT"
     gene            2844..4118
                     /locus_tag="ACHK74_004881"
     CDS             2844..4118
                     /locus_tag="ACHK74_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP0223556.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSAT
                     VKTISRTPASTQEMLTYKGAPQNTPGDSVPWYKVIAMPEKQIIRYDEKYLPLENGMKA
                     ESTLFLEKRRIYQWMLSPFYDMKHSATGPLND"
     gene            4111..6213
                     /gene="mchF"
                     /locus_tag="ACHK74_004882"
     CDS             4111..6213
                     /gene="mchF"
                     /locus_tag="ACHK74_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
                     /protein_id="HFP0223557.1"
                     /translation="MTNGNFRQIINQLDMRWRRRVPVIHQTETAECGLACLAMICGHF
                     GKNIDLISLRRKFNLSARGANLAGINGIAEQLGMVTRALSLELDELGALKMPCILHWD
                     FSHFVVLVSVKRNRYVLHDPARGRRYLGREEMSRYFTGIALEVWPGSEFLAETQQTRI
                     SLRSLINSIYGIKRTLAKIFCLSVVIEAINLVMPVGTQLVMDHAIPAGDRGLLTLISA
                     GLMFFILLRAAVSMLRAWSSLVMSTLINIQWQSGLFNHLLRLPLAFFERRKLGDIQSR
                     FGSLDTLRATFTTCVVGAIMDSIMVVGVFVMMLLYGGYLTWIVLGFTMVYVLIRLVTY
                     GYYRQISEETLVRGARASSYFMESLYGIATVKIQGMAGIRGTHWLNLKIDAINSGIKL
                     TRMDLLFGGINTFVAACDQVAILWLGASLVIDNQMTIGMFVAFGSFRGQFSDRVASLT
                     SFLLQLRIMSLHNERIADIALHEKEEKKPEIEIVADMSPVSLETTDLSYRYDSQSAQV
                     FSGLNLSVAPGESVAITGASGAGKTTLMKVLCGLFEPDSGKVLVNGTDIRQLGINNYH
                     RMIACVMQDDRLFSGSIRENICGFAEETDDEWMTECAKASHIHDVIMKMPMGYETLIG
                     ELGEGLSGGQKQRIFIARALYRKPGILFMDEATSSLDTESERFVNAAIKKMNITRVII
                     AHRETTLRTVDRIFRITN"
     gene            complement(6274..6597)
                     /locus_tag="ACHK74_004883"
     CDS             complement(6274..6597)
                     /locus_tag="ACHK74_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223558.1"
                     /translation="MRNITTDELALIHGAGLDKDASVAIGTAAGGFLGKATKIPGADI
                     AGSAIGGYIGGVIADGSNRTITIPSNGINYNPGIGLGSFNPNYNGGLLNSGFNSSTSS
                     SSSGS"
BASE COUNT         2044 a         1287 c         1656 g         2066 t
ORIGIN      
        1 ttgttggcga agggaattct tatgttgacg tttttctaag ctttataata tttcttggtt
       61 ttattgagct gattcatggg attcgaagga ttctggtctg gtcaggctgg aaaaacggaa
      121 gttaaatatg atggagttta tatgcagttt atatgcagtt tatatgcgag aaataacaga
      181 atcacagtta agatatattt ccggggcggg aggtgcgcca gcgacttcag ctaatgctgc
      241 aggtgctgca gctattgttg gagctctcgc cggaatacct ggtggtccac ttggggttgt
      301 agttggagcc gtatctgccg gtttgacaac agcaattggc tcgaccgtgg gaagtggtag
      361 tgccagttct tctgctggtg gcggtagcta attatatttt tatttatttt acaggcactt
      421 tttcggtagt acctgtaaaa tatatccttt ttgtttaaaa cagatagaag atccatttat
      481 aagtgtgttt ttatgttcaa cactttactg atgaagatat gatttacagt cgttgataaa
      541 taatttttta atttatgtgt aattaataga gttttactgt aataaggaag aagctgtcag
      601 gacacttact tgctctttta tgaacactct tttatgaaaa ttgaaacgga gaatactgcc
      661 agatgagtca tcagtgttca ctttctgaac tgaatgaaaa cttggtgcct ttcactgcca
      721 ggcagatcaa ggcctcatta atctggtgtg cagaggatgt cagaaatcca gacgagctgc
      781 aaaatgcctg cagttatatt atcgatcctg gcagtacagc ttctgccaaa gtgttccatg
      841 cagagcgcta tggtggcagt ggtattcagc gtaatggagg tggtgcacgt tgtgggtttg
      901 atggtaacta ccaggttaaa ggaataggaa gtaatccgtt ggttggcgaa ggtactgacg
      961 aacgtcattc taatggtgca ctcggcgctg ttcatgcaat atatgaggct ttgtggggag
     1021 aagtactggc tcaaatatta ccttatagtg ctgtgcgggt tcgggcggtt ttacttacag
     1081 atctctatac tgaaaaggca tttgagcgct ccggtaggaa atcacgaaga gccctgttgg
     1141 tacgtgagcc tgttgttcgt ccggcgcatt ttgaacgggc accttacttc caagtaaaac
     1201 cggagtattc cagtctgtta attcacgatg cctgtcgggt tagatctgtg atccacaagc
     1261 tgccaggata tctacctgta ccaccggaag aaattgatgc tgaagcacga actgatcccc
     1321 ggatttattg cattgagggg ttatgtgaac tggcacgtcg tgaggcctgg caaatggcat
     1381 tttgtcgaac acgtttcctg agattgacaa cttctccttc taatattgca atggatggca
     1441 gattaatgga ttttaacgga ctcagttgtc tgtttccggg agattcccca gctgattttg
     1501 ggtataaact aagattagct gaactggcaa aagaaccgat ggtacttatg caagggctgt
     1561 ctgatctctg cttgtatatc ggaaaatata tgtttgaccc tgacttcact cttgcagccc
     1621 gtttgaaggt tgaggagata tttcagaaaa cttttcatga agcatgttat tactgttatc
     1681 tagaactgtt gggtattcct ggagaattta taacacaaaa agagatacct gatatattga
     1741 aagaactggt taacagtttt gttgcattac tcaatcaata ctgcgagaaa tcacatgccc
     1801 aagatattgt caatcaggat ggttcaccat tgcaaaagtt ggttgtgacg ctaatccatc
     1861 ataggcataa tcaaaagcag gcactgaata gtagcatcaa gaatgatgtt catttcaccg
     1921 ttgcacaaca gtgtttttcc caggccatcc actggctgac gcaaggcagt accagacgtc
     1981 agataaatgc ctcattactc ctgaaagaaa ttgaacatca taccatgaaa aggctgcaac
     2041 ccagggaaga gctgaggaaa gaaaatatgt gcgaaaaaat tgccatcctg ctggataatc
     2101 atggcgatga tccccttttt ttacaagaag caatttctga tatgaaaaat tttatgctta
     2161 agttttccag agatgcattt ggatatcttg aaccgataag aaacacagtg taaaaaatat
     2221 ataagggaaa tagtaatcat gtcttatata agggaaacca tcagaggaaa agatgaatgg
     2281 actgtttatg aacagatcgg ttttgcggtc agttgtatgc tctacaatcg taattacagt
     2341 ctgtatccgg tgttaaccat tcaatactgg actgaatatg cgatacagca taatcagatt
     2401 aaattcctgt ttgattcacg aggttttcca ctggcgtata ttacctgggc atatcttgag
     2461 gctgatacag aagcgcgcct gctcagggat ccagaattca ggttgcatcc gtctgaatgg
     2521 aatgaagatg gaaggatttg gattctggat ttctgttgta aaccaggctt tggtcgaaaa
     2581 gttattgact atctcataca gcttcagcca tggggagaag gagaagtacg atggttaagc
     2641 aggcgaaaga aaattgtgac atacatccct gagcggctgc ataaaacgta gtacctctga
     2701 agatacagag ataattgtaa attacggggt aaatgcatcg ctgatactat tttgacagga
     2761 ctctgtattt tctggctttg attatagcat ttcgcttaac cagtgaaggt attacaggaa
     2821 aagtgtgagt aagaggagct attttgtttc gtcaggatgc tttagaaaac agaaaaatga
     2881 agtggcaggg acgggcaata ttacttcccg gaataccact atggttaatc atgctgggaa
     2941 gcatagtgtt tattacggca tttctgatgt tcattattgt tggtacctat agccgccgtg
     3001 ttaatgtcag tggtgaggtc acaacctggc caagagctgt caatatatat tcaggtgtac
     3061 agggatttgt tgtcaggcag tttgttcatg aaggacagtt gataaaaaaa ggggatcctg
     3121 tttatctgat tgacatcagt aaaagtacac gtagtggtat tgtcactgat aatcatcgtc
     3181 gggatataga aaaccagctg gttcgtgtgg acaacattat ttcccgtctg gaagaaagta
     3241 aaaaaataac gctggatacc ctggaaaaac aacgcctgca atacacagat gcgtttcgtc
     3301 gctcatcaga tattatacag cgtgcagagg aagggataaa aataatgaaa aacaatatgg
     3361 agaattacag aaactatcag gcaaaagggc tgattaataa agatcagtta actaaccagg
     3421 tggcattata ttatcagcaa caaaacaatc ttctcagcct gagcggacag aacgaacaga
     3481 atgccctgca gataaccact ctggagagtc agattcagac tcaggctgca gattttgata
     3541 accgtatcta ccagatggaa ctgcaacggt acgagttaca gaaagaactg gttaacactg
     3601 atgtggaggg cgaaatcatt atccgggcgt tgactgacgg gaaagttgac tccctgagtg
     3661 tcactgtcgg gcaaatggtc aataccggag acagccttct gcaggttatt cctgagaaca
     3721 ttgaaaacta ttatcttatt ctctgggtcc cgaatgatgc tgttccttat atttcggctg
     3781 gtgacaaagt gaatattcgt tatgaggcct ttccggcaga aaaatttggg cagttctctg
     3841 ctacggttaa aactatatcc aggactcctg cgtcaacaca ggaaatgttg acctataagg
     3901 gagcaccaca gaatacgccg ggcgactctg ttccctggta taaagtcatt gcgatgcctg
     3961 aaaagcagat tatcagatat gacgaaaaat acctccctct ggaaaatgga atgaaagccg
     4021 aaagtacact atttctggaa aaaaggcgta tttaccagtg gatgctttct cctttctatg
     4081 acatgaaaca cagtgcaaca ggaccgctca atgactaacg ggaatttcag acaaattata
     4141 aatcagcttg atatgcgctg gcgacgtcgt gttccggtta ttcatcagac ggagaccgct
     4201 gaatgtggac tggcctgcct ggcaatgata tgcggtcatt ttggtaagaa tattgacctg
     4261 atatctcttc gccggaagtt taatctctcg gcccgtggag caaaccttgc aggaatcaat
     4321 ggaatagcgg agcagctggg gatggtcacc cgggctcttt cactggagct ggatgaactt
     4381 ggtgccctca aaatgccgtg tattctccac tgggatttca gtcacttcgt cgtgctggtc
     4441 agcgtaaagc gtaaccgtta tgtactgcat gatccggcca gaggcagaag atatctcggt
     4501 cgggaggaaa tgagccggta ttttacgggc attgcacttg aggtctggcc cggaagtgaa
     4561 ttcctggcgg aaacccagca gacccgcata agtctccgtt cactgattaa cagtatttac
     4621 ggtattaaaa gaacactggc gaaaattttc tgtctgtcag ttgtaattga agcaatcaat
     4681 ctggtaatgc cggtgggaac tcagctggtt atggatcatg cgattccggc gggggacaga
     4741 gggctgctga cgcttatttc tgctggcctg atgttcttta tattgctcag ggccgcggtg
     4801 agtatgctgc gtgcatggtc ctcactggtt atgagcacgc tcatcaatat acagtggcag
     4861 tcgggtctgt ttaaccatct tctcagactg ccgctggcgt tttttgaacg ccgtaaatta
     4921 ggtgatatcc agtcgcgttt tggctccctt gacactttga gggccacctt tactacctgt
     4981 gttgttgggg caatcatgga cagtattatg gttgtgggtg tttttgtgat gatgctgtta
     5041 tatggaggat atcttacctg gatagtgctc ggttttacca tggtttacgt tcttattcgt
     5101 ctggtgacat acggctatta ccggcaaata tcggaagaaa ctcttgtcag gggggcccgg
     5161 gccagctcct attttatgga aagcctgtat ggtattgcca cggtaaaaat ccagggtatg
     5221 gctgggattc ggggaacaca ctggcttaac ctgaaaatag atgcgatcaa ttcaggtatt
     5281 aagttaacca ggatggattt gctcttcggg gggataaata cttttgttgc cgcctgtgat
     5341 caggtggcga ttttatggct gggtgcaagc cttgtgatcg ataatcagat gacaataggg
     5401 atgtttgtgg catttggttc ttttcgtggg cagttttcgg atcgggttgc ttcgctgacc
     5461 agttttcttc ttcaactgag aataatgagt ctgcataatg agcgcattgc agatattgca
     5521 ctacatgaaa aggaagaaaa gaaaccggaa attgaaatcg ttgctgacat gagcccggtt
     5581 tcactggaaa ccactgattt aagctaccgg tatgacagcc agtcagcaca ggtattcagt
     5641 ggtctgaatt tgtctgtggc tccgggagaa agtgtggcta taactggtgc ctccggtgcc
     5701 ggaaaaacca cattaatgaa agtattatgt ggactgtttg aaccagatag tggaaaagta
     5761 ctggttaatg gcacggatat acgtcaactt ggaataaata attatcaccg tatgatagcc
     5821 tgtgttatgc aggacgaccg gctattttca ggatcaattc gtgaaaatat ctgtgggttt
     5881 gcagaagaaa cagacgacga atggatgaca gaatgtgcca aagcaagtca tattcatgat
     5941 gtgataatga aaatgccaat ggggtatgaa acgttaatag gtgaactggg ggaaggtctt
     6001 tccggcggtc aaaaacagcg tatattcatt gcccgagctt tataccggaa acctggaata
     6061 ttatttatgg atgaggctac aagttctctt gatacagaaa gtgaacgttt cgtgaatgct
     6121 gctataaaaa agatgaatat cacccgggtg attattgcac atagagagac aactttaaga
     6181 acagttgatc ggatttttag aattacaaat taggataata tatcactaaa agcagcattg
     6241 ctgcataata agctatgctg ctttagaaat taatcaactt ccactactag aagaggatgt
     6301 tgatgagtta aatcctgagt ttaataaacc tccattgtag tttggattaa aacttccaag
     6361 tcctataccc ggattatagt taataccgtt tgatggtatt gttattgtcc tatttgaacc
     6421 atcagcaata actcctccaa tatatcctcc aattgcactt cctgctatat ccgcaccagg
     6481 gatttttgtt gctttaccta aaaaaccacc agccgcagta cctattgcaa cggatgcatc
     6541 tttatctaat cctgcaccat gtattaaagc aagttcatcg gttgttatat ttctcatgtt
     6601 atttcctctt tttattaaag aaagataaaa caaataaaaa cagtacactc gcaagtaacg
     6661 aaagaattat tttctttatg aaaagataaa gggttaatgt gtctaagtct gaatttataa
     6721 gttgtattat aaagaacaac gagaaataaa aaaatagttt cattattaaa ccatccctat
     6781 acatgagtct ttgagtgctt atgaaaaact tagaaataaa gactatagaa accaaggtag
     6841 caaaagtttt tatttatccg aatattttta cttcttgctg tttgtatatc ttttttattg
     6901 ttgtaaaata gttcgcgcaa tagataaaat aataggaata ccaattaatt ataccaattg
     6961 cttttactgc aaaccgtgat ttaaatcaca tttatataga tgtgttataa gtttcaagtc
     7021 tctttctacg agaaaaggcg aagatatttg ttc
//
LOCUS       DAZGSP010000180         7006 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZGSP010000180 DAZGSP010000000
VERSION     DAZGSP010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.181"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..255
                     /gene="yafQ"
                     /locus_tag="ACHK74_004884"
     CDS             <1..255
                     /gene="yafQ"
                     /locus_tag="ACHK74_004884"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFP0223559.1"
                     /translation="SGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPL
                     QGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            411..1151
                     /gene="dpaA"
                     /locus_tag="ACHK74_004885"
     CDS             411..1151
                     /gene="dpaA"
                     /locus_tag="ACHK74_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFP0223560.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1122..1889)
                     /gene="yafJ"
                     /locus_tag="ACHK74_004886"
     CDS             complement(1122..1889)
                     /gene="yafJ"
                     /locus_tag="ACHK74_004886"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFP0223561.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2095..2673)
                     /gene="lpcA"
                     /locus_tag="ACHK74_004887"
     CDS             complement(2095..2673)
                     /gene="lpcA"
                     /locus_tag="ACHK74_004887"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFP0223562.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2913..5357
                     /gene="fadE"
                     /locus_tag="ACHK74_004888"
     CDS             2913..5357
                     /gene="fadE"
                     /locus_tag="ACHK74_004888"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFP0223563.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5400..5846)
                     /gene="ivy"
                     /locus_tag="ACHK74_004889"
     CDS             complement(5400..5846)
                     /gene="ivy"
                     /locus_tag="ACHK74_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFP0223564.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6027..6794
                     /gene="yafV"
                     /locus_tag="ACHK74_004890"
     CDS             6027..6794
                     /gene="yafV"
                     /locus_tag="ACHK74_004890"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFP0223565.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRL"
     gene            complement(6915..>7006)
                     /locus_tag="ACHK74_004891"
     CDS             complement(6915..>7006)
                     /locus_tag="ACHK74_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223566.1"
                     /translation="LNTCYIEEVLASCSELGFRTDKMKNLTQI"
BASE COUNT         1644 a         1760 c         1885 g         1717 t
ORIGIN      
        1 tcgggacaat tttcaaagga tgtaaaactt gcacaaaagc gtcataagga tatgaataaa
       61 ttgaaatatc ttatgacgct tcttatcaat aatgctttac cacttccagc tgtttataaa
      121 gaccacccgc tgcaaggttc atggaaaggt tatcgcgatg ctcatgtcga accggactgg
      181 atcctgattt acaaacttac cgataaactt ttacgatttg agagaactgg aactcacgcg
      241 gcgctctttg ggtaaagcac gctgccgatg aaatttgtaa accctgcgcc gacaggcttg
      301 cgtaaaactc ttttggtgat aatgtgagcc ttccaaatcc agactattcc gatttgggat
      361 ctattttgcc gtcctggcgt gtgtaaccgt tttatcaagg aataagcagt atgcgtaaaa
      421 tcgcattaat tcttgcgatg ttgttgatcc cgtgcgtttc gtttgccggt ttgctgggca
      481 gcagtagctc gaccacgcca gtgagcaaag agtataagca gcagttgatg ggatcccctg
      541 tctacatcca gatcttcaag gaagaacgta cgctcgatct ctacgtcaaa atgggcgagc
      601 aatatcagct gctcgacagt tataaaatct gtaaatattc cggcggctta gggccaaaac
      661 agcgtcaggg cgatttcaaa agcccggaag ggttttatag cgtccagcgt aatcagttaa
      721 aaccagacag ccgttactac aaagcgatta atattggttt ccccaatgcc tatgaccgtg
      781 cgcatggtta tgaagggaaa tacctgatga tccacggcga ttgtgtttcc atcggctgct
      841 acgcaatgac caatcagggt attgatgaga tcttccagtt cgttactggt gcgctggtgt
      901 ttggtcagcc gagcgtgcag gtgagtattt acccgttccg catgaccgac gccaatatga
      961 agcgccataa atattccaac tttaaggact tctgggagca actgaagccg ggttacgact
     1021 actttgagca aacccgtaag ccaccgaccg tttctgtggt caatggtcgt tacgtggtca
     1081 gcaagccgtt aagccacgaa gttgtgcagc cacaactggc atcaaactac acgctccccg
     1141 aggcaaaata agcgccattc gcctggcata atcttttgcc aggtttcatt gcccgtcagc
     1201 ggctgtgttg caatcaccgt gaccacatca ttcggtgtgg tctgcgagct gaagtcgatt
     1261 tccacatcct gatccagcag cgttgccacg ccaaacggcg cgcggcgggt gatccagtgt
     1321 aaattagtcg agcaatacgc catcacatag cgcccgtcag aaagcagcat gttgaaaacg
     1381 cccttctgcc gcagttcatc cgccagtgag gcgatatatt taaacactgc cgccatgttg
     1441 cccggtgtgc gcgggtaacg ctgcgttaat ttatgcagga gccagcagaa ggctttttcg
     1501 ctatcggttt caccgaccgg gcggaagttg ccggtttcca gtgatttgta gcccgtcagt
     1561 tgtccgttat gggcataagt ccagttacgc ccccataact cgcgggtaaa tgggtgagta
     1621 ttttccagcg ccacctcgcc ccgattagcc tggcgaatat gagccaccac cgaacaggat
     1681 tttatcgggt agtcctggac aagtttggcg atgggggaat taaagctggg ttgtggatct
     1741 ttaaatgtgc gacagccttt accttcgtaa aaggtaatgc cccagccatc tttatgtggc
     1801 ccggttccac caccacgctg tacaagcccg gtgaaactaa agcagatatc ggtagggacg
     1861 ttggcgctca tcccgagcaa ttcgcacatg ggtaacctcc acaacagcgg gagcgcatgg
     1921 cccccggcga agattgattt aagaattttc aggtcggatg cggcgtgaac gcattattcg
     1981 gcctacggtt cgcgtacaga tttgtaggca tgataagacg cgtcagcgtc gcatcaggca
     2041 tcggtgcaca attgccggat gcggcgtaaa cgtcttatcc ggcctacgcc agacttactt
     2101 aaccatctct ttttcaatca actggatcag gatatggatc actttaatgt gaatctcctg
     2161 aatgcggtcg gcataaccaa agtgcggtac gcgaatttcg atatccgccg tgccagccat
     2221 tttgccgccg tctttaccgg tcagggtgat cactttcatt cccttctcac gcgccgctgc
     2281 gatcgctttg atcacgtttg cagagttacc ggaggtggag atccccagca gtacatcgcc
     2341 ttcgcgacct accgcttcaa cgtaacggga gaaaatatca ttgaaaccga aatcgttacc
     2401 gacgcatgaa atatgactaa cgtcagaaat agcaatcgcc gggtagcccg gacggttttc
     2461 acggtagcga ccggtcaact cttcggcaaa gtgcatagcg tcgcaatggg aaccgccgtt
     2521 gccgcaggaa agcactttgc caccggcttt aaagctgtct gctaacagga ccgccgcgcg
     2581 ctgaatggcg tgaatattgg cgtcatcttt taaaaagtta gccagcgttt ccgccgcttc
     2641 gttcagttcg ttacgaataa gatcctggta catgaggata tccttcagca taaatgtaat
     2701 agacaaaatg cagtgtaccg gataccgcca aaagcgagaa gtacgggcag gtgctatgac
     2761 caggactttt tgacctgaag tgcggataaa aacagcaaca atgtgagctt tgttgtaatt
     2821 atattgtaaa cacattgcta aatgttttta catccactac aaccatatca tcacaagtgg
     2881 tcagacctcc tacaagtaag gggcttttcg ttatgatgat tttgagtatt ctcgctacgg
     2941 ttgtcctgct cggcgcgttg ttctatcacc gcgtgagctt atttatcagc agtctgattt
     3001 tgctcgcctg gacagccgcc ctcggcgttg ctggtctgtg gtcggcgtgg gtactggtgc
     3061 ctctggccat tatcctcgtg ccatttaact ttgcgcctat gcgtaagtcg atgatttccg
     3121 cgccggtatt tcgcggtttc cgtaaggtga tgccgccgat gtcgcgcact gagaaagaag
     3181 cgattgatgc gggcaccacc tggtgggagg gcgacttgtt ccagggcaag ccggactgga
     3241 aaaagctgca taactatccg cagccgcgcc tgaccgcaga agagcaagcg tttctcgacg
     3301 gtccggtaga agaagcctgc cggatggcga atgatttcca gatcacccat gagctggctg
     3361 atttgccgcc ggagttgtgg gcgtacctta aagagcatcg tttcttcgcg atgatcatca
     3421 aaaaagagta cggcgggctg gagttctcgg cttatgccca gtctcgcgtg ctgcaaaaac
     3481 tctccggcgt gagcgggatc ctggcgatta ccgtcggcgt gccgaactca ttaggcccgg
     3541 gcgagctgct gcaacattac ggcactgacg agcagaaaaa tcactatctg ccgcgtctgg
     3601 cgcgtggtca ggagatccct tgctttgcac tgaccagccc ggaagcgggt tccgatgcag
     3661 gcgctatccc ggataccggg attgtctgca tgggcgaatg gcagggccag caggtgctgg
     3721 ggatgcgtct gacctggaac aaacgctaca ttacgctggc accgattgcg accgtgcttg
     3781 ggctggcgtt taaactctcc gacccggaga aattgctggg tggcgcagaa gatttaggta
     3841 ttacctgtgc gctgatccca accaccacgc cgggcgtgga aattggtcgt cgccacttcc
     3901 cgctgaacgt gccgttccag aatggaccga cgcgcggtaa agatgtcttc gtaccgatcg
     3961 attacattat cggcgggccg aaaatggccg ggcaaggctg gcggatgctg gtggagtgcc
     4021 tctcggtagg ccgcggcatc accctgcctt ccaactcaac cggcggcgtg aaatcagtag
     4081 cgctggcaac gggcgcgtat gctcacattc gccgtcagtt caaaatctct atcggtaaga
     4141 tggaagggat tgaagagccg ctggcgcgta ttgccggtaa tgcctacgtg atggatgctg
     4201 cggcatcgct gattacctac ggcattatgc tcggcgaaaa acctgccgtg ctgtcggcta
     4261 tcgttaagta tcactgtacc caccgcgggc agcagtcgat tattgatgcg atggatatca
     4321 ctggtggcaa aggcattatg ctcgggcaga gcaacttcct ggcgcgtgct taccagggcg
     4381 caccgattgc catcaccgtt gaaggggcca acattctgac ccgcagcatg atgatcttcg
     4441 gacaaggagc gattcgttgc catccgtacg tgctggaaga gatggaagca gcgaagaaca
     4501 atgacgtcaa cgcgttcgat aaactgttgt tcaaacatat cggtcacgtc ggtagcaaca
     4561 aagttcgcag cttctggctg ggcctgacgc gcggtttaac cagcagcacg ccaaccggcg
     4621 atgccactaa acgctactat cagcacctga accgcctgag cgccaacctc gccctgcttt
     4681 ctgatgtctc gatggctgtg ctgggcggca gcctgaaacg tcgtgagcgc atctcggcac
     4741 gtctggggga tattttaagc cagctttacc tcgcctctgc cgttctgaag cgttatgacg
     4801 acgaaggccg taatgaagcc gacctgccgc tggtgcactg gggcgtacaa gatgcgctgt
     4861 atcaggctga acaggcgatg gatgatttac tgcaaaactt cccgaaccgc gtggttgccg
     4921 ggctgctgaa tgtggtgatc ttcccgaccg gacgtcatta tctggcacct tctgacaagc
     4981 tggatcataa agtggcgaag attttacaag tgccgaacgc cacccgttcc cgcattggtc
     5041 gcggtcagta cctgacgccg agcgagcata atccggttgg cttgctggaa gaggcgctgg
     5101 tggatgtgat tgccgccgac ccaattcatc agcggatctg taaagagctg ggtaaaaacc
     5161 tgccgtttac ccgtctggat gaactggcgc acaacgcgct ggcgaagggg ctgattgata
     5221 aagatgaagc cgctattctg gtgaaagctg aagaaagccg tctgcgcagt attaacgttg
     5281 atgactttga tccggaagag ctggcgacga agccggtaaa gttgccggag aaagtgcgga
     5341 aagttgaagc cgcgtaattg aataacggag ccgaaaggct ccgtttcttt atccgctaat
     5401 tatttaaaat taaagccatc cggatggttt tccaggctgc cggtcaacgc cgcgaacagc
     5461 accgttttac catcaatcga aagcgcatcg ttcacattca gccaggtgag tttctcttgc
     5521 gacgttttct catcaatagt cgagaacagc cccgtcatct gattagattt ctcggaccac
     5581 atcacagcga tacgttgcga gccacagtca tgcggtttgc acgcgctcat cacctgatac
     5641 gtctcatctc ccaacgttac ggtttgtgcg ggagtataag taccgccttt catcacccag
     5701 gcaggcagct tatgcccttg taccatctga ttaaatgcag ctttggtggt ttcgcccttt
     5761 gcaaggctgc taatggttaa atcatcctgc gccattgcac tggtggcgat gaccagagcg
     5821 gcgactgtcg ttattgcctt aaacatcatt cctcccgagc ttatcctgcc catgttatta
     5881 accttctaaa gcctggcgat aacacgataa tttgcaaatc agatatttcc aatatcagca
     5941 tttgttatca atttattctg cgctaaggca actgcctgat aaggtgttaa agttataaaa
     6001 cccttcgcat aaaggagttg tacaccgtgc ctggtttgaa gattacgctt ttgcagcaac
     6061 cactggtgtg gatggatggc cctgccaacc tgcgtcattt tgatcgtcaa ctggaaggta
     6121 ttaccgggcg cgatgtgatc gttctaccgg agatgtttac cagcggcttt gccatggaag
     6181 cggcagcttc gtcgctagca caagatgacg tagtgaactg gatgacagct aaggcgcagc
     6241 agtgcaatgc gctgattgca ggcagtgttg cattacaaac ggagtctggt tcggttaacc
     6301 gctttttgct ggttgagccg ggcggcacgg tacattttta tgataagcgt catctgttcc
     6361 gcatggcaga tgagcatcta cattataaag cgggcaatgc gcgagtgatt gtggaatggc
     6421 gcggctggcg tattttgccg ctggtgtgct acgacttacg ttttcctgtg tggtcgcgca
     6481 atctcaacga ttatgacctc gccctgtacg tcgccaactg gcctgctccg cgctctctgc
     6541 actggcaggc attgctgacg gcccgcgcga ttgagaatca ggcgtatgtg gcgggatgca
     6601 atcgcgtcgg cagcgatggc aacggctgcc attatcgcgg tgacagccgg gtgattaatc
     6661 cacaaggcga gattatcgct actgccgacg cgcatcaggc aacgcgcatt gatgccgagc
     6721 tgtcgatggc ggtgttaagg gaatatcgcg ataagtttcc ggcatggcgg gatgcggatg
     6781 agtttaggtt gtagtgagtg ggggctgtat ttagggcggg gaattcagaa agggatgttt
     6841 agtgtgctga gcgagagtta aataataaaa aactgggaag atttaataat ccccccgcag
     6901 ggcaagagca tgaattaaat ttgtgttaaa tttttcattt tgtcagttcg aaacccaagc
     6961 tctgagcagg atgcaagcac ttcttctata taacatgtgt ttaacg
//
LOCUS       DAZGSP010000181         6846 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZGSP010000181 DAZGSP010000000
VERSION     DAZGSP010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.182"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..488
                     /locus_tag="ACHK74_004892"
     CDS             <1..488
                     /locus_tag="ACHK74_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP0223567.1"
                     /translation="IANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSKSAAGTVSQP
                     GRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRCAYCGHTAKE
                     NRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQAT
                     A"
     gene            complement(528..1742)
                     /gene="ydcO"
                     /locus_tag="ACHK74_004893"
     CDS             complement(528..1742)
                     /gene="ydcO"
                     /locus_tag="ACHK74_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFP0223568.1"
                     /translation="MLRCLYAITYEVTMRLFSIPPPTLLAGFLAVLIGYASSGAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            1795..2331
                     /gene="sutR"
                     /locus_tag="ACHK74_004894"
     CDS             1795..2331
                     /gene="sutR"
                     /locus_tag="ACHK74_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFP0223569.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2404..4365
                     /gene="rlhA"
                     /locus_tag="ACHK74_004895"
     CDS             2404..4365
                     /gene="rlhA"
                     /locus_tag="ACHK74_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFP0223570.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTRENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     HRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(4457..4687)
                     /gene="yncJ"
                     /locus_tag="ACHK74_004896"
     CDS             complement(4457..4687)
                     /gene="yncJ"
                     /locus_tag="ACHK74_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFP0223571.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4909..5085
                     /gene="hicA"
                     /locus_tag="ACHK74_004897"
     CDS             4909..5085
                     /gene="hicA"
                     /locus_tag="ACHK74_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFP0223572.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            5131..5547
                     /gene="hicB"
                     /locus_tag="ACHK74_004898"
     CDS             5131..5547
                     /gene="hicB"
                     /locus_tag="ACHK74_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFP0223573.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5626..>6846
                     /locus_tag="ACHK74_004899"
     CDS             5626..>6846
                     /locus_tag="ACHK74_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP0223574.1"
                     /translation="MKKYQQLAEQLREQIATGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKEPVIPVTRDEAVDINTYIFDMLQASRE
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTELGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPE"
BASE COUNT         1776 a         1720 c         1732 g         1618 t
ORIGIN      
        1 gtatcgcaaa tatccgcagg gactaccttc acaaagtcac aacggccgtc agcaaaaacc
       61 acgcaatgat agtcattgag gatttgaagg tcagcaacat gtcaaagtca gcagcgggta
      121 cggtcagcca gccggggcgc aatgtccggg caaaatcagg tttaaaccgt tcgatactgg
      181 atcagggctg gtatgaaatg cgccgccagc ttgagtacaa gcagctctgg cgtggcggtc
      241 aggtgcttgc tgttccgcca gcgtatacaa gccagcgttg cgcgtactgt ggtcatacag
      301 cgaaagagaa ccgcctgtca caaagtaaat tcagatgcca ggtatgtgga tatacagcga
      361 acgccgatgt aaatggcgct cgtaacattt tagcggcggg gcacgccgtt cttgcctgtg
      421 gagagatggt gcagtcaggc cgcccgttga agcaggaacc caccgaaatg attcaggcga
      481 cagcctgaac gtagcaggga tccacgtcct tcagggcgtg gaggatgtca atatcggttt
      541 ctgtcagcga ttaaattcaa caccacgtaa caaacgcctc cggcaattaa tccccagaac
      601 gcagaaccaa tcccgaccag cgtcaatcca cttgccgtta ccagaaatgc caccaccgcc
      661 gcgtctcgct cacgctcatt atgcagcgcc tgatacaaac tgccgctaat ggtacttaac
      721 agcgccagac ctgccagcat ctggatccaa cttacgggca gggcagccat catcccggta
      781 atagcactac caaacagacc tgcgatcaaa taaaaaatgc ctgcaacggc agctgccagc
      841 caacgttgat ctttatccgg atgcgcttcc gggctttggc aaatagccgc ggtgattgcc
      901 gcaataccga cggaataaac gccgaaaggg gaaaaaacca gtgccagcaa tccagtaaat
      961 acaattaatg gcgaaacagg agccgaatac ccggctgctt tcattgctgc gatacccggt
     1021 gcgttttgcg atgccatcgt caccagaaaa agggggagtg caacgctcag gctgtgagca
     1081 aacgaaaaat caggggtaat ataagtgggg agaacgggtt taaagacaac atcagttgtg
     1141 acaacgtcac cttgcgcgat aacgatcacg atcccaataa tcatcgcggc aattaccgca
     1201 tagcgcggcg caacggcctt ggttgccagc cataccagca acatacttcc acacaacgta
     1261 aattgaccgt ccagactggc aaacgcctgt aaaccaaagc gtaataaaat cccggcaagc
     1321 attgccgccg caagcgagtg cggaataatg cgcatcagac gagcaaagag tcccgttatg
     1381 ccgcagagga ctattaacgc gttggtgaca ataaaaacgc cgatggcttc gttaagtgtt
     1441 agtccctgca atccggtgac caacaaagcc gcgccaggcg ttgaccatgc ggtgagaaca
     1501 ggtacgcgat accataatgt cagagtcagc gtactgacgc ccattgccag ccccagcgcc
     1561 gtcatccagc cagagatttg tgcagtggtg gctccggcga caatcgctgc ttgccagatt
     1621 attgcccctg aactggcgta gccaattaat accgccagaa accccgccag tagcgtgggt
     1681 ggaggaatag aaaacagacg catagtcacc tcgtacgtta tagcgtacag gcaccgtagc
     1741 atttgtccgt tataacgcac aagtgataaa cttccgtttt gccggaggag tcgcatggaa
     1801 aatctcgctc gctttttatc caccacactt aaacaactac gccagcagcg cggctggagt
     1861 ctttcgcgac tggcagaagc gacgggcgtt tctaaagcaa tgttagggca gattgagcgt
     1921 aatgagtcca gcccgacggt cgcgacatta tggaaaattg ccaccgggtt gaatgtgcca
     1981 ttttcgacat ttatttctcc gccacagtcc gctacgcctt cagtttatga tccacaacag
     2041 caggcgatgg tgatcacttc gctgtttcct tatgatccac aactctgctt tgaacacttc
     2101 tcgattcaga tggcacccgg tgcaataagc gaatcgacgc cccatgagaa gggggtaatc
     2161 gaacatgtcg tcgtcattga tggacaactt gatctgtgcg ttgatgggga atggcagacc
     2221 cttaattgtg gggaaggcgt tcgatttgct gcagacgtca cgcatatcta tcgtaatggt
     2281 ggggagcaaa ccgtacattt tcattccctc atccattacc cgcgcagtta agcgggaaaa
     2341 ctatttcgca acgtcgcgct tgtggctaaa atagccgcca tttttcagct actggataag
     2401 aatgtgaccg tatcttctca tcgacttgaa ctgttaagcc cggcacgcga tgccgccatt
     2461 gcccgcgaag ctattttgca cggtgccgat gctgtttata tcggcggccc tggttttggt
     2521 gcccgtcata atgccagtaa tagcctgaaa gatattgccg agctggtgcc gtttgcccat
     2581 cgttatggtg caaaaatttt cgtcacgctt aacaccattt tgcatgatga tgagctggaa
     2641 cccgcgcaac ggctgattac tgacctctac cagaccggtg tcgatgcgct gattgttcag
     2701 gatatgggga ttctggaact tgatattccg ccgattgaac tgcacgccag tacgcagtgc
     2761 gacattcgta cagttgaaaa agcgaagttc ctctctgatg ttggcttcac gcagattgtg
     2821 ctggcgcgag agctgaatct tgatcagatc cgcgcgattc accaggctac ggacgcgacc
     2881 attgaattct ttattcatgg ggcactgtgc gtggcctatt cgggtcagtg ctacatttct
     2941 catgcgcaaa cagggcgtag cgccaaccgt ggcgattgct cgcaggcgtg ccgtttgcca
     3001 tacacattga aagacgatca ggggcgggtg gtttcctatg aaaaacatct gctgtcgatg
     3061 aaagataacg atcagacagc caacctcggc gcgctgattg atgctggcgt acgctccttc
     3121 aagattgaag ggcgttacaa agatatgagc tacgtgaaga atatcaccgc ccattatcgc
     3181 cagatgctgg atgccattat tgaagaacgt ggcgatctga cgcgtgcttc atcaggtcgt
     3241 actgaacatt tctttgttcc atcgacggaa aagactttcc accgtggtag cacagattat
     3301 tttgtgaatg cccgtaaagg cgatattggc gcgttcgatt cgccgaaatt tatcggcctg
     3361 ccggtgggtg aagtattgaa agtggcgaaa gatcatctcg atgttgccgt caccgagcca
     3421 ctggcaaatg gtgatggcct gaacgtgatg attaaacgtg aagtcatcgg ttttcgtgcc
     3481 aatacggtcg agaaaaccag agaaaatcag taccgcgtct ggcccaatga aatgccagca
     3541 gatttgcaca aaattcgtcc acatcaccca ctaaaccgta atcttgatca taactggcag
     3601 caggcactga caaaaacctc cagcgaacgt cgggtggcgg tagacattga attgggcggc
     3661 tggcaggaac aactgattct gaccctcacc agtgaagagg gtgtcagcat cacgcatacg
     3721 ctggacgggc agttcgacga agccaataac gccgaaaaag caatgaacaa tctgaaggat
     3781 ggtctggcaa aactggggca aaccctctat tacgcccgcg atgtgcaaat taatttgccg
     3841 ggggcgctgt ttgtaccaaa cagtctgtta aaccagttcc gccgtgaagc tgctgacatg
     3901 ctggatgctg cgcgtcttgc cagttaccat cgcggcagcc gtaaaccggt tgctgatcct
     3961 gcgccggttt atccgcaaac gcatctgagt ttcctcgcga acgtatacaa ccagaaagcg
     4021 cgtgaatttt atcatcgcta tggtgtgcag ctgattgacg cggcgtatga ggcacatgaa
     4081 gagaagggcg aagtcccggt gatgatcacc aagcattgtc tgcgctttgc ctttaatctg
     4141 tgcccgaaac aggcgaaagg caatatcaaa agctggaagg cgacgccaat gcaactggtt
     4201 aacggcgatg aagtattaac gctaaagttt gattgccgcc catgcgagat gcacgtcatt
     4261 ggcaaaatca aaaatcacat actgaaaatg ccgttaccgg gaagcgtagt ggcatccgta
     4321 agtccggatg agctgctgaa aacattgcct aagcgaaaag ggtaaaacgc cagttttctg
     4381 gttactcaca actcattgaa tctgcatgat attgcctgcc gggtaaggtg tcacgccgca
     4441 tccggcaaca aatgactcaa cgttgtcccg cttctggttt gcgcgatttt tgccagtaaa
     4501 aatgctcgcg caaaccttcc gccgactctt ccgccacagc acgcaattca tcgctgtccg
     4561 cttcatgacg cagctgatga tccacattct ttacccacac aaattcatgt cctttgtgcc
     4621 ctgccatgag ttgtcctgag aacagcgcac acgttaataa gacaaccgat aacgccttcg
     4681 taaacatcct gccacctttt tcttaccttt tgccgctatg atgccgatcg tttcttgagg
     4741 ttattattca gttttgcaaa ttagcgcaaa gaaattctgg aatcttcctt cctgattttg
     4801 catggcattc tgccgttgcg gcgatttagt gctattttcg agcaaattac acacggaggt
     4861 aaacactaat gtttactttg ttgatatact cagcggcagg gaggcgatgt gaaacaaagc
     4921 gagttcagac gttggctcga atctcagggc gtcgatgtag cgaatggcag caaccatttg
     4981 aaactcaggt ttcatgggag gcgcagtgtc atgccgcgtc acccctgcga tgagattaaa
     5041 gaaccattgc gtaaagcaat cctgaaacaa ctcggtttga gttaatcgcc aattaaaaag
     5101 gttaatgaca tgcgagggac agtcgaaatt atgcgttatc ccgtcactct tacacccgcg
     5161 ccggaaggcg gttatatggt ttcttttgtg gatatccctg aagcgttgac gcagggcgaa
     5221 actgtcgctg aagcgatgga agcggcaaaa gatgctttac tgaccgcatt tgatttttat
     5281 tttgaagata acgagcttat cccgttacct tcgccattaa atagtcatga tcactttatt
     5341 gaagtacctt tgagcgtcgc ctctaaagta ttgctgttaa atgctttttt acagtcagaa
     5401 atcactcagc aagagttagc caggcgaatt ggcaaaccta aacaggagat tactcgccta
     5461 tttaatttgc accatgcgac aaaaatcgac gccgtccagc ttgcggcaaa ggcgcttggc
     5521 aaagagttat cgctggtgat ggtttaatta cagttaacga aaagttgtca tttttaacaa
     5581 ctgatataga ctgccgaatc atctgcacat aattacgatt cgataatgaa aaaataccag
     5641 cagcttgcag aacaattacg cgagcagatt gctacgggta tctggcaacc cggcgatcgt
     5701 ttgccttcgt tgcgtgacca ggtggcgctt tcaggcatga gctttatgac tgtcagccat
     5761 gcctatcagt tgctcgaaag tcagggatat attatcgcac gaccgcagtc gggttattac
     5821 gttgcgccac aggcaataaa aatgccgaaa gagccagtca ttccagtcac tcgagatgaa
     5881 gcagtcgata tcaacactta tatttttgat atgttgcagg ccagtcgcga accgtcggtc
     5941 gttccgtttg cctcggcctt tcccgacccg cgacttttcc ccctccaaca actaaaccgc
     6001 tcgctggcgc aggtaagcaa aaccgccacg gcgatgagcg tgattgaaaa cttaccgcca
     6061 ggaaacgcag aactgcgtca ggctattgct cgtcgctatg ccttacaggg catcaccatt
     6121 tctcctgatg aaattgtcat tactgccggg gcgttagagg cattaaacct cagtttgcaa
     6181 gcggtaactg aactgggcga ttgggtgata gtagagaatc cttgtttcta tggcgcgttg
     6241 caggcgctgg aacggctacg gctgaaagcg ttatcggtgg cgacggatgt taaagaaggg
     6301 atagatcttc aggcgttgga actggcgttg caggagtatc cggtgaaagc gtgctggctg
     6361 atgactaata gccagaatcc actcggattt accttaacgc cgcaaaaaaa agcacaactg
     6421 gtggcgttgc tcaatcagta caacgtaacg ctgattgaag atgacgttta cagcgaactt
     6481 tattttggac gggaaaaacc gctgcctgcg aaagcgtggg atcgccacga tggcgttttg
     6541 cattgctctt cgttttcgaa atgtctggtg cctggttttc gtattggttg ggtcgccgcc
     6601 ggaaaacatg cacgtaaaat tcaacgattg cagttgatga gtacgctttc caccagctca
     6661 ccgatgcagc ttgcgctggt ggattacctt tccacgcgcc gatacgacgc ccatcttcgt
     6721 cgcctgcgtc gccagcttgc ggaacgtaaa caacgtgcct ggcaggcact gctgcgttat
     6781 ctgcctgcgg aagtgaaaat tcatcataat gacagtggtt actttctctg gctggagctc
     6841 cccgag
//
LOCUS       DAZGSP010000182         6761 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZGSP010000182 DAZGSP010000000
VERSION     DAZGSP010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.183"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1918)
                     /locus_tag="ACHK74_004900"
     CDS             complement(<1..1918)
                     /locus_tag="ACHK74_004900"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HFP0223575.1"
                     /translation="MTTAITSLKHKYPHAPAEEQLPYWVEILLVDEQGDAVSDMPWKV
                     ESHHPGDGIIKQFTYTGRSGSDGLIRIDMPHGLELKLTLDADSLAKEMEKRPLRVGRD
                     AEMDSLIRQLAEDKGYIWHYAVIGELCKVTPSIKLESGEGWPPYHFPHGKSFKGFIIR
                     TNELERRHVIEICPFRAWELVLHHQKDYSIANGINLGVAATLAYADDNALSKSSITNF
                     FINQCQDLSRLPRLYKDKSSWNTLVRDVPYSERYSPPVFMDTSIDSSSKEDKDNQQGT
                     ISTKNDVSNVKADGDTQLYYVYNSDKIIIAWRGTESLFDAGTDIAFNPVKTESCDVNK
                     TQCAALVPAGKVHNGFWSGYLRLGRVFNNELDELLTLIKSRDLFVCGHSLGGALALIH
                     SAALKLEKPLLYTYGMPRTFTRNAIMELSDITHFRHINDNDPIPAVPMEANLDNEWYK
                     LWGLLGGTLGFFWSLGELMAYQLVAWGDCFWHHGNTVAFLTVTQSREWKEYKRNLPSP
                     AGGITIRKRLPVKAKLYLVPVLAEQEMQEAGQKQKEFQASLTKTDLTRFFPQGGNPER
                     GVNINIFEHFMTSYMPYMYNKLLELIDKAGIVEKRTFTEHLYNIDLFKMQMEENKGCI
                     PDKEFSRNKIFLDIE"
     gene            complement(1921..4452)
                     /locus_tag="ACHK74_004901"
                     /pseudo
     CDS             complement(1921..4452)
                     /locus_tag="ACHK74_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054501.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
     gene            complement(4439..4981)
                     /locus_tag="ACHK74_004902"
     CDS             complement(4439..4981)
                     /locus_tag="ACHK74_004902"
                     /inference="COORDINATES: protein motif:HMM:NF045617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative T6SS immunity periplasmic lipoprotein"
                     /protein_id="HFP0223576.1"
                     /translation="MVKIRAFRYRSALLLCLTLLTGCPGRGDYYRFDETVTVSTRGDN
                     VCFSVPEPEDYQPVSVSIAPRGTRFRERQITSEPSLRIVNGQLCIPPSFCLFPDKGQF
                     IVRSVLYSERDKDTPRIMVAGIEIADGCIFDIPLNDMEVVRPYGELKSSNVQTDQSER
                     HGPCKYPFRAVRGVINHESH"
     gene            complement(4975..5541)
                     /locus_tag="ACHK74_004903"
     CDS             complement(4975..5541)
                     /locus_tag="ACHK74_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFP0223577.1"
                     /translation="MTGYGRNQDAGAGKNQREEGSLRLLTPGEIALARLVFRSTIPYE
                     KVWIHHDSFLPFGLQNKQTAMVPDGKIYFREHYRDDYSLSVPFYQHMFIHEMAHVWQR
                     EKGMNVIGRGMFSWAVNYRYVLDGRLLSEYPMEQQAQIIADHFTLQAEGYGTWCDMRR
                     DGDITLDGNMSEYVIRSLYTSTLRGFPW"
     gene            complement(5538..>6761)
                     /locus_tag="ACHK74_004904"
     CDS             complement(5538..>6761)
                     /locus_tag="ACHK74_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0223578.1"
                     /translation="GERLSAIEQEENVLMTARDDLEQQYARECELTGELLESRSDISR
                     QSETLHLQQALHDIQQNQPLLSVDVDVRTVAGVVADWTGVPLSSLMKDEQTELLHLEK
                     DIGRRVVGQDVALGSIAQRLRAAKTGLTSGNGPQGVFLLVGPSGVGKTETALALADVM
                     YGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGILTEAVRKRPYSVVLLDEVE
                     KAHRDVLNLFYQVFDRGFMRDGEGREIDFRNTVILMTSNLGSDLLMQQLSEKPETTES
                     ELHELIRPRLRDHFQPALLARFQTVIYRPLTQSAMRTIVEMKLAQVCERLHCHYGLST
                     SVDERVYDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSHMAAKQKPQALALAWS
                     DEDGMVIELRQECAL"
BASE COUNT         1703 a         1788 c         1449 g         1821 t
ORIGIN      
        1 tttcaatatc aaggaatatt ttattcctgg aaaattcttt atccggtatg caacctttat
       61 tttcttccat ttgcattttg aacaagtcaa tgttataaag atgttcggtg aatgtacgtt
      121 tttcaactat cccagcttta tcaattaatt caagcaactt attgtacata taaggcatat
      181 atgacgtcat aaagtgctca aaaatattta tgttcactcc tcgctcagga ttaccacctt
      241 gtggaaaaaa acgagtcaaa tctgtttttg ttagtgacgc ctgaaattct ttctgtttct
      301 gacctgcctc ctgcatttcc tgttctgcca atacaggtac taagtaaagc ttagctttaa
      361 cggggagtcg ttttctgata gtaatccccc ctgcagggct tggcaggttt cttttatatt
      421 ctttccattc ccgactctga gtgaccgtaa gaaaagctac tgtattaccg tgatgccaaa
      481 aacagtctcc ccaggccacc aactgataag ccatgagttc acccaatgac caaaagaaac
      541 ccagggtacc accaagcaat ccccaaagtt tgtaccactc attatccaga ttagcttcca
      601 tgggaactgc tggaatcgga tcattatcat taatatgtcg aaaatgtgta atatccgata
      661 actccattat ggcatttcga gtaaaggtcc gaggcattcc gtaagtataa agcagaggtt
      721 tctctaattt taatgctgca gaatgaatta atgccagagc tccccctaaa ctatgtccac
      781 aaacaaacaa atcccttgat ttaattaacg ttaaaagctc atcaagttca ttattaaata
      841 ctcttccaag tctgagatat ccactccaaa acccattatg cacttttcct gcaggcacta
      901 atgctgcaca ttgtgtttta tttacatcac atgactctgt ttttactgga ttgaatgcaa
      961 tgtctgtacc cgcatcaaac aaactttctg tacctcgcca agcaataatt atcttatctg
     1021 aattataaac ataataaagc tgcgtatcac catccgcctt tacatttgaa acatcattct
     1081 tagtagaaat agttccctgc tgattatctt tatcctcttt agatgaggaa tctattgacg
     1141 tatccataaa tacgggtggt gaataacgtt cactataagg cacatcccgg accagcgtat
     1201 tccaggatga cttatcttta taaaggcgag gcagacggga aagatcctga cactggttaa
     1261 taaaaaaatt cgtaattgaa gatttactta aagcattatc atccgcataa gcgagtgttg
     1321 ctgcaacgcc aagattaata ccatttgcaa tggaataatc tttttgatgg tgcagaacaa
     1381 gttcccatgc cctgaaaggg catatttcaa taacatgtcg cctttcaagc tcatttgttc
     1441 tgataataaa tcccttaaat gacttgccgt gaggaaaatg ataaggaggc cagccctccc
     1501 cactttcgag cttaattgat ggagtaacct tacaaagctc accaataaca gcataatgcc
     1561 agatatatcc tttatcttct gctaattgcc gaatgagtga atccatttct gcatccctgc
     1621 caacccgaag agggcgtttc tccatctctt ttgccagtga atcagcgtcc agtgtaagtt
     1681 tcaactcaag accatgtggc atatcaattc ttataagacc atccgaaccg cttcgcccgg
     1741 tatatgtaaa ctgcttaatt attccatcac cagggtggtg actctccact ttccatggca
     1801 tgtctgatac agcatcccct tgttcatcca caagcagtat ttcaacccaa tagggtaatt
     1861 gttcttctgc cggagcatga ggatatttgt gtttaagaga agtaattgca gtagtcatga
     1921 ttattcttct tccttgttct ctgatacatt acgcgggtat tcaatcttta tctgagaggg
     1981 cattgatgta aaaattgatg atgttttacc ttctgaatca gaaataccct catacacttc
     2041 cccttcactt gttgtgatcc tgtatgttgt aaaaggttta atttttccac tctgacgtga
     2101 agtcgtaatg aacgtttctc catatccttc aggaaatgaa accggaggcg tatttaaaga
     2161 tgcaggacct gtttgatcta catttgtagc cttgagcaga atgttaccgg gacatcccag
     2221 ttcaatattg cctccactca atttgatata tgcaccatta caattcaata taagttcttt
     2281 agcagcatta attgaaatac tgttctcggt actgctgacc gtcatatccc tgagggatga
     2341 aagattgagc tcatccccct gagcctgtat atctacttta ccgtcccccg cgaatagctg
     2401 cataccagca tcacgggcaa ataaacttat cgtatctgta gccgcaacag tgaaggattc
     2461 gcctgtactg atatctgtat ttcttccgga catcagaccg atactttgtt ctcctgacgc
     2521 cagacgaacg gtctgtgggc taagaatacc tattccagaa ggtgcatgtg ccagtaatcc
     2581 tggcattttc aggtatccca gcgcactatt aagcttcttc tgaccgtcaa tatccgatgg
     2641 cgtagctttt gctcctttta ctgccgcctg caaatttcgg gcaagtgtca gagcttgggt
     2701 caattgctca attgccgcat ccatttccag cattttactt ccgacatcag gctgagtatc
     2761 tgcggtcagt aatatcccct tccctccccg cacgctgacc cagtcgtttg tacgcagctc
     2821 tgcaccttcc ccccgaagtt cccgggaggc attaacgtta tggccaagat taagttgtgt
     2881 cttaccgcca tactcggtac tgagcttgat atgctcctca ccgcgtttgt cctccatccg
     2941 caacttgtta tttgccggcg tacggataac attgcgcgta ccgttcttat cggtaacgtg
     3001 atcaacatga cgtgaatcat gcagggcatg cgctatatag ggacgatccg gatcaccttc
     3061 ctcaaaagca atggccactt ccgttccctg aatcagcgga aaatgaaaac cataagcatc
     3121 accactgtag ggtttggcca gacgtaccgg catactttcc tgtcccggat ttttatcatc
     3181 acggtcagca tcaaatttca cccggtacat gcctgaagca tcctgccagg cgtagatatc
     3241 cccttctttt gcactggtca cacgggccgt catggtcccg gtgattttcg gacgagtaag
     3301 taacggtggt cgccagcaga caatctcgct gtaaggaaca gcactcagca tcacctgtag
     3361 cgcagatttc cggctaccgg tgaacagaag ctacgtgagc agaaccggct cctgtaacgg
     3421 tgcaggcaac actggtggag tactgtcagt aatagtcagc acctgtgccg gtaacagact
     3481 ggcatcggtg cttttacctg tgatacgtgt ttgctcagcc agaaaacgct cgtgatcgag
     3541 ccgggcccag aagttagccg tctccggcgc agggtcaatt ttatcgcccc tttctgtatg
     3601 gcgtggccgg taatgataaa cctcgccata actggtgtcg taaccttctc cacgggtcat
     3661 gtccgccggt acggacatca gtatgttctg ggcctcgcgg tggttatagt cgttcgtgat
     3721 aacacggctt tccaccaccc ggtgctccac attcaatgcc catactgagt ctgtgccact
     3781 gtcactcata ccggacgggc tgttaacggg cagtgtttta tcaaatatca acgccgcctg
     3841 ggcgtcaccg aagtggatca cctctgtctg cgcatccggg tgcaacgtaa agaagtaaaa
     3901 aatgcccact tctgccagca ggcgctggat aaatctcaga tcgctttcct gatactggtt
     3961 aatctgttcg cgcttcggat acgtcctttt gaggctgaac tcatactccc agcctttcag
     4021 cccgtgttcc gtcaggattt gctccaccac ctcaggcact gatttattaa cgaaaaaacg
     4081 gtgtgtacgg aactgtttat ccagcaggga aatgaaaggt ttaagtgtga tccggtattg
     4141 tgcctggtcc tcagagccag aaatacgtct gaaatcagta ataacaccgt gcactttttt
     4201 ctgttcagtc aggctttcca gtaatccggt tcccattatc agggttgcgg atttacgcag
     4261 gaaccaggca gcatcaggat acctgtccgt actggtaaaa atgatgtcat accggtaaat
     4321 cttacttatg ccttcagttc cgctgaaatc ttcaacatcc agcgatgacg ggcaggaagg
     4381 tatatccagc ctgtagcggt tcagggttcc caccccggat aaaacatttt gcagggagtc
     4441 agtgagattc atgatttatc actccacgaa cagctctgaa agggtatttg cagggaccat
     4501 gacgttcgct ctggtcagtc tgaacattac tgcttttcag ctcaccgtat ggcctcacga
     4561 cctccatgtc attaagcggg atatcgaaaa tacacccgtc ggcaatctct atgccggcaa
     4621 ccatgatgcg cggtgtgtcc ttatcgcgct cagagtacag aacagacctg acaatgaact
     4681 ggcctttatc aggaaaaaga cagaatgaag gcggaatgca cagctggccg ttcacgatac
     4741 gaagcgaggg ttcagatgta atttgccgct ccctgaatcg ggttccccgt ggagcgatgg
     4801 aaacgctgac tggctggtag tcctctggct ccgggacact gaaacacaca ttgtcgcctc
     4861 ttgtgctgac cgttactgtt tcatcaaacc gataataatc cccgcgtccg gggcatcctg
     4921 tcagtaacgt cagacagagc agtaaagcag aacgataacg aaaggctctt atttttacca
     4981 tgggaacccc cgcaacgtgc tggtatacag gctgcgaata acatactcag acatatttcc
     5041 gtccagtgtg atatcaccgt cccttctcat atcacaccat gtcccgtatc cttcagcctg
     5101 caaagtaaaa tgatcagcaa taatttgtgc ctgctgctcc atgggatatt cgcttaacag
     5161 acgcccgtcc agaacataac ggtaattcac cgcccaactg aacatccccc gaccgatgac
     5221 attcattccc ttttcacgct gccagacatg cgccatttca tgaataaaca tatgctgata
     5281 aaagggaacg ctgagtgaat aatcatcccg atagtgctca cggaaataaa tcttaccatc
     5341 ggggaccatg gcagtttgct tattctgaag accaaaaggc agaaaactgt catggtggat
     5401 ccagaccttt tcataaggga ttgtcgacct gaataccagc ctcgccagcg caatctctcc
     5461 cggcgtcagt aatcgcaatg agccttcttc cctttgattt ttacctgcgc cagcgtcctg
     5521 atttctgcca tagcctgtca taacgcgcat tcctgccgca gctcaatcac cataccgtct
     5581 tcgtcactcc atgccagagc cagcgcctgt ggtttctgct tcgcggccat atggctcagc
     5641 aactgccggc tcagtaccgg cagcagctgc tggttcagca gactctccac attccgggcc
     5701 cccgtatccg gcagcaggca ggctgatgtc agggcatcgt acacacgttc atcaaccgat
     5761 gtgctcagtc cataatggca gtgcaggcgt tcgcagacct gtgcaagctt catctccaca
     5821 atggtgcgca tggcagactg cgtcagcgga cggtaaatca cggtctgaaa gcgagccagc
     5881 agggcaggct ggaagtggtc gcgcagccgt ggacggataa gctcatgcag ctccgattcc
     5941 gttgtctccg gcttttcgct cagttgctgc atcagaaggt cactgcccag attggaggtc
     6001 atcagaatga ccgtattacg gaagtcaatt tcacgcccct caccgtcgcg cataaagccc
     6061 cggtcaaaca cctggtagaa cagattcagc acatcccggt gggccttttc cacttcatcc
     6121 agcagcacca cactgtacgg acgcttacgt acagcttccg tcagaatacc cccctgaccg
     6181 tatcccacgt acccgggcgg tgaaccctta agctgggaaa ccgtatgggg ttcctggtat
     6241 tccgacagat tgatggtaat cagtgatttt tcgccgccgt acatcacatc tgccagcgcc
     6301 agtgccgttt cggtttttcc cacaccactg ggaccgacca gcaggaacac gccctggggg
     6361 ccgttaccgg atgtcagacc cgttttcgcc gcacgcagac gctgcgcaat ggatcccagt
     6421 gccacgtcct gtccgaccac ccgtctgccg atatcctttt ccagatgcag cagttccgtc
     6481 tgttcgtcct tcatcaggga agataacggc accccggtcc agtccgcgac cacaccggca
     6541 acggtgcgta cgtcaacatc cacactgagc aggggctgat tctgctgaat gtcgtgcagt
     6601 gcctgctgca gatgcagtgt ctcactctga cgggaaatat cgctgcgact ttcaagtaac
     6661 tcaccggtga gttcgcactc ccgggcgtac tgctgctcca ggtcatcacg tgctgtcatc
     6721 agcacattct cttcctgctc aatggcagac agacgttcgc c
//
LOCUS       DAZGSP010000183         6746 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZGSP010000183 DAZGSP010000000
VERSION     DAZGSP010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.184"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(179..1108)
                     /gene="fdhE"
                     /locus_tag="ACHK74_004905"
     CDS             complement(179..1108)
                     /gene="fdhE"
                     /locus_tag="ACHK74_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFP0223579.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1105..1740)
                     /gene="fdoI"
                     /locus_tag="ACHK74_004906"
     CDS             complement(1105..1740)
                     /gene="fdoI"
                     /locus_tag="ACHK74_004906"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP0223580.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1737..2639)
                     /gene="fdoH"
                     /locus_tag="ACHK74_004907"
     CDS             complement(1737..2639)
                     /gene="fdoH"
                     /locus_tag="ACHK74_004907"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFP0223581.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2652..5702)
                     /gene="fdnG"
                     /locus_tag="ACHK74_004908"
     CDS             complement(2652..5702)
                     /gene="fdnG"
                     /locus_tag="ACHK74_004908"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5115..5117),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP0223582.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            5896..6729
                     /gene="fdhD"
                     /locus_tag="ACHK74_004909"
     CDS             5896..6729
                     /gene="fdhD"
                     /locus_tag="ACHK74_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFP0223583.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1463 a         1834 c         1838 g         1611 t
ORIGIN      
        1 tcttacgctt gtcatcagtg acataccaat aaccttatcg atgtgttgga gggaagaacg
       61 aggcaaacag gcactggcgg catagcttga ggtggtaacg cggaatacgg gagatggtgt
      121 cgtcacaccc tatgggctgt aatccgggcg acctgcgcca cccggaaaac gtcagagatt
      181 actccccttc acccgggaac aggaacgggt tgatggaact gcgggcgtag ccttcttgct
      241 ccattcgcgc gtccagtacc agagaggcga ggtcatctgc cacggcttca actttcggtt
      301 ctttttcctg ataaagaatc ttcaggtaag tgccgcagtc atcgcagctt tcggctttaa
      361 tcgcggcctg ttcgtcatcc agcgaccagt aatgcagttt gccgctctgc tcgcagttgc
      421 tgcattttac gcgcactacg tgccattcgg tttcacacag gttgcagtgc aggtaacgca
      481 gaccctgagt ggtgccaatt tgcaccatgc tggacaccgg catagaacca caaaccgggc
      541 aatattgacg ttgctcgccg tattcagcgc gggctttgcc ggggatcaga ttggccatct
      601 gcgcccagta gagcgacagt gcagcccaga taaacggcgc tttatcgctg ctgacggatg
      661 agaaatcaga ggcaaacagt gcgctggcca tatcttccag ctcctgagtt gatgccttct
      721 ccagattctc aatcactgcc agcgccgggc cgctcatttc aggtttcagc tcagcaatca
      781 gcgccatcag cagcttttgc cagtgcttat cacgcggcag aacgtgaata tccagcgggg
      841 gcttgccctg tgcgctggct tctttaatgc gcgcggtcag atccatctcc agcggatggt
      901 cgtacagcac cacttcctgg gcgtgggcga taagcgcagc aaagcgcagg taatcaccca
      961 gcggattatt ttctgccagc tcgcgcagac gctcggcgcg gcggttgtat aaattcttga
     1021 gccgagggaa caataatggc ggaatcatat ccgccgtacg tttctcgctc gaacccagct
     1081 catcttgcgg gattatgcga atactcattc agctttcttt tccgttgtct tgcggacctc
     1141 acggtaccag cgcgggtgat gtttcttcgc ccaggcgctg gtaacccatc cttccaccat
     1201 ggcggtaatc gtgcctttca cccaaagggc ggcgtagata tgcaccatga taaccacaat
     1261 taacgccact gcggcaaatg aatgcagcat taacgcgaat cggatcaccg ggattgagaa
     1321 agcaggcgca aaataaggac gccagataat cacgccgctc accagcagca gaaccaggaa
     1381 aataatcgcc gcccagaaaa cgcatttctg accgaagtta taacgcccgg tgtcacctac
     1441 ttcctcgttg acgacgatct tacgaatatt cttcgcccaa aagatatcat cccgattgat
     1501 taggttgtga tgccagtaac ggaaaaacat gatgatgaac gaggcaaaca taaccacgcc
     1561 gacaaacggg tgcagaattc gcgccagctg cggtgtgccc atgatttgca tcaaccagtt
     1621 gaaggacggg aacaaaaagc ccagcccgct caccgccgcc aggatgaagc agaaggcggt
     1681 gatccagtgg ttgatacgtt ccggcgcggt gtagcgcacg atggtgtcac gtcgtttcat
     1741 ttgcgctcct cgtctttctc ttcgtgcaga ttattctctt cctcatccgc acggttcgga
     1801 ccgacaccca cgtagtggaa gatactggcc gcaaaggtag ccgcaaagcc aacagccgcg
     1861 agcggtttcc agatgccttt ccagaatttc acggtttcgc tgatttccgg gttctccggc
     1921 aagccatgat acagatttgg cttgtcagca tggtgcagca cgtacatgac gtgtgtacca
     1981 ccgacgcctg ccggatcgta cagacccgca ttgtcgtaac cgcgggtttt cagctcagca
     2041 acgcgctcgc tcgccagcgt tttcatcgac tctttcgtac cgaagtgaat cgcgcccgtt
     2101 gggcaggtct tcacgcaggc tggttcttgc ccaaccacca cgcggtcaac gcacagcgta
     2161 catttgtaga cgcggttgtc ttccgggttg aggcgcggaa tgtcgaacgg acagcccgca
     2221 atgcaataac cgcagccaat gcactgctcg gactggaagt cgacgatacc gttggcatac
     2281 tgaataattg caccttccgc cgggcacgct ttcaggcagc ctggatcgga acagtgcata
     2341 cagccgtctt tgcggatcag ccattccagt ttgtcgttct gctccacttc cgagaagcgc
     2401 atcaccgtcc acgatttggc gcttaaatca ttggggttgt cgtacacccc aatattattg
     2461 ccgacggtat cgcgaatgtc gttccactct gaacacgcca cctgacaggc tttacagccg
     2521 atacaggtgg tgacgtcgat gagtttcgcc acttcttcct ggaagtcccg cgcctgaggc
     2581 gcgggggtca gaccgttagt cgcggaacga cgaatgatat cttgagattg ataagccata
     2641 tgtcgtctcc gttacacctt ttccacattc acaaggaagg acttaaactc cggcgtctgc
     2701 gtgttcgcat caccaacgaa tggcgttaac gtattggcaa taaagccttt tttagctacg
     2761 ccttcataac cccagtgaat cggaataccg atggtatcga tatctttgcc gttcgctttc
     2821 agcgtacgaa tacgtttggt caccaccgct ttggctttga tatagccacg gttggaggag
     2881 actttcacgg tatcgccctg ggcaatgcca agtttattcg ccagcgactc cccgatttcc
     2941 acaaactgct ctggttgcaa aatcgcgttc aacagcgcgt gtttggtcca gtagtggaag
     3001 tgctcggtca gacgataggt ggttccgaca tacgggaact tatcggcttt acccaatgct
     3061 tcggcgtcgt ctttaaagat acgtgcagcc gggttcgaga taacgtttgg atgcagcggg
     3121 ttagttccca gcggcgtttc aaacggctcg tagtgttccg ggaacggacc ttccgccatc
     3181 ttatcgaggg caaacagacg ccccatgcct tcctgctgca tgataaacgg cccgacgccg
     3241 ctacccggag gcgctgcgct gtagtccgga atatcccagc cggtccactt agtgccgtcc
     3301 catttcagca actgacgctt cggatcccac gggttaccct gcggatctgc ggaggcgcgg
     3361 ttatacagaa tgcggcggtt aagcggccat gcccatgccc agcccagcgt gttaccgagg
     3421 ccagacggat cggcgttatc acgacgcgcc atctggttgc cttccggcgt ccagctaccg
     3481 gcgaaaatcc agcagccaca ggaggttgta ccgtcatcgc gcaattgggc gaacgagcta
     3541 agttgttggc ctttcttgac gataaccgca ccggttgccg gatcggtaat atcggccagc
     3601 gccttaccgt tgctctccat cgctacttct tccgacttcg gctcatgcgg aatggcgtag
     3661 ttccaggtca tgttcagcac ctggtccggg ttcgcgccac cctgttcggc atacatcttg
     3721 cgcaagcgca ggaagatacc ggagaggatc tcgccatcag tcagcgcaat ccccggggcg
     3781 tccgcacctt tccagtgcca ctgcaaccag cgacctgagt taacgattga accgttctct
     3841 tctgcgaagc aggtcgatgg cagacggaac acttcggtct ggatcttcga cgagtcaact
     3901 tcgttcagct caccgtggtt ctgccagaag ttagaggttt cagtgttcag tgggtcgatg
     3961 gtgacgagga acttcagttt cgacagacaa ccgatcactt tgtttttgtt cgggaatgag
     4021 gcaacagggt taaagccctg gcagatatag ccattgacct tgccctcttt catcatctcg
     4081 aagtactgca ggacgtcgta gcctttatcc cacttcggca accagtcaaa gccccagcta
     4141 ttttccgccg tcgctttatc accaaagaag gccttcatca tagagacgaa gaatttcggg
     4201 tagttgcccc agtagtttac ctggccttcc agcagcggtt ttggcgtgtt ggcggtaagg
     4261 taggtttgca gatcggtctg cttctcgctt ggcagcgtca tgtaacctgg cagactctgc
     4321 gacagtagcc ccaggtccgt cagcccctga atattggagt gaccgcgcag ggcgttaacg
     4381 ccgccgccgg ccatccccat attgccgagc agcagctgga tcatcgccat cgtacgaatg
     4441 ttttgcgcac caacggagtg ttgcgtccag ccgagggcat acaggaacga ggcagtttta
     4501 tcgtgagcac tggtttctgc gatgtattcg cagactttca ggaacgcgtc ttttggcgta
     4561 ccacagatgt tttcaaccac atctggcgtg tagcgggaaa cgtgctgttt cagcaagttc
     4621 cacacgcagc gcggatgttg cagcgtggta tcgcgtttgg cgaagccgtt ttcgtccagt
     4681 tcataagtcc aggaggattt atcgtacttg cgtttttccg cgtcgtagcc ggtgaacagg
     4741 ccatcttcaa agccgtaatc ctcacgcact atcaggctgg cgttggtata ggcttcggtg
     4801 tattcgcggt tgaatttttc attgttcagc aggtacagca atacgcctga caggaaagca
     4861 atgtcagtac cggaacgaat aggggcatag tagtcagcta ccgccgccgt acgcgtaaag
     4921 cgaggatcga tcacaatcag cttcgcgccg ttgtgaattt tggcttccat cgcccagcgg
     4981 aacccgaccg ggtgagcttc agcggcgtta ccgcccatca ccacgacgag gttggcgttc
     5041 ttgatgtcga cccagtggtt ggtcatcgca ccgcgaccaa atgttggagc aagacttgct
     5101 accgttggtc cgtgtcagac acgcgcctgg ttgtcgaccg cgagcatacc cagcgcgcgg
     5161 gagaattttt gcgttaaata gccggtttcg ttactcgacg cggaagcaca cagcatcccg
     5221 gtggagagcc agcggttaac agtcacgcct tcggcgtttt gcgcaatgta gttagcatcg
     5281 cggtcttctt tcatcagttt ggcgatgcga tcaaacgcct cttcccaact gatttgctgc
     5341 catttatcag aacctggcgc acggtattcc ggaaacttca gacggctttc ggagtggatg
     5401 aaatccacca ggccagcgcc tttcggacaa agtgcaccgc ggttgaccgg gtgatccgga
     5461 tcgccttcga tatggaagat agatgctttg gcgttttttg ctccgtcacc gaggctgtac
     5521 atcaacagcc cacagcctac ggaacaatag gtgcaggtat tacgggtttc gcgggtgcgc
     5581 agcagtttat actgccgggt ttccgcgagc gctacgctgg gtgcaaaacc cagtgccgct
     5641 gccgtggtgc ctgccatacc gccagcgcag atcttaaaga actgccttct gctgacctgc
     5701 atggattgct ccttgtttcg acattgtcac gtcccattta cattcgcttg ctgcgtgtgc
     5761 agggagtggg agttattttt ctttgcggaa ggggccgcaa aggtccagaa ttggctcaat
     5821 ttccctccat ccaggaaggg tttgtaacag aataccataa tgttggtgtg tgtgttctta
     5881 tctggttaag agaaagtgaa aaaaacacag caaaaagaaa tcgaaaatgt gacaaatatc
     5941 acaggtgttc gtcaaattga gttatggcgg cgtgatgatt tacaacaccc acggttagat
     6001 gaggtcgcgg aagaagttcc cgttgcgctg gtctacaacg gcatttcgca tgtggtgatg
     6061 atggcgtcgc ccaaagacct tgagtacttt gcgctcggtt tttcgctttc cgaggggata
     6121 atagaaagtc cgcgcgatat cttcggcatg gatgtcgttc cttcctgtaa tggtcttgaa
     6181 gtacaaattg agctttccag ccgccgcttt atggggttga aggagcgccg ccgggcgctg
     6241 gcgggacgta cgggctgtgg cgtatgcggc gtggagcaac ttaatgacat cggaaaaccg
     6301 gtgcagccgc taccgttcac ccagacgttt gatctcaaca aactggatga tgcattacgt
     6361 catctcaacg attttcagcc agtggggcaa ctgactggtt gtactcacgc cgctgcctgg
     6421 atgttgccat ctggcgaact ggtcggcggg catgaagacg tgggtcgcca tgtggcgctg
     6481 gacaaactgt taggccgccg gtcacaagaa ggggaaagct ggcagcaagg tgcggtactg
     6541 gtttccagtc gtgccagtta tgaaatggtg caaaagtcgg cgatgtgcgg cgtagagatt
     6601 ttgtttgcgg tgtctgccgc gaccacgctt gctgtagaag tggctgagcg ttgtaatctg
     6661 acgctggtgg gtttttgtaa gccgggtcgg gcaacggttt atacccatcc gcagcgtttg
     6721 agcaattaat taaaataagt cctgcg
//
LOCUS       DAZGSP010000184         6744 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZGSP010000184 DAZGSP010000000
VERSION     DAZGSP010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.185"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..205
                     /locus_tag="ACHK74_004910"
     CDS             <1..205
                     /locus_tag="ACHK74_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP0223584.1"
                     /translation="KKICRAEGATEEDDNKLVREFERLTEHPDGSDLIYYPRDDREDS
                     PEGIVKEIKEWRAANGKSGFKQG"
     gene            267..410
                     /locus_tag="ACHK74_004911"
     CDS             267..410
                     /locus_tag="ACHK74_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFP0223585.1"
                     /translation="MKKITGIILLLLAVIILSACQANYIRDVQGGTVSPSSTAEVTGL
                     ATQ"
     gene            905..1819
                     /locus_tag="ACHK74_004912"
     CDS             905..1819
                     /locus_tag="ACHK74_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP0223586.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAIERALCEKLGADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASARRSIDKHYGMGRNCYLFEMTRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPV
                     PLSPAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQATRGRKGGSKSKRGTVAT
                     SARTLKPWEKLGISRAWYYQLKKRGLVE"
     gene            complement(2044..2310)
                     /locus_tag="ACHK74_004913"
     CDS             complement(2044..2310)
                     /locus_tag="ACHK74_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HFP0223587.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRR
                     PEEAQLRAFLLQFLRKEVTRQADVNRIQPLINELEKLPKPPAKP"
     gene            2489..4708
                     /gene="mobQ"
                     /locus_tag="ACHK74_004914"
     CDS             2489..4708
                     /gene="mobQ"
                     /locus_tag="ACHK74_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFP0223588.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTR
                     KRGVDDAVILTPANVPSWCSDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAR
                     EAVLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNERAL
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETE
                     RGEQNRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASA
                     DTLREFIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKA
                     RPVASLLSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFET
                     QWNTWIAPGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETAL
                     VGVITELGRAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGA
                     VMVSDIREGMPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNF
                     YFSDDRRPVASPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVG
                     FVRREGGFCPVLVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQF
                     RLKGHHDRLLAAVHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
     gene            4941..6686
                     /gene="col"
                     /locus_tag="ACHK74_004915"
     CDS             4941..6686
                     /gene="col"
                     /locus_tag="ACHK74_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="HFP0223589.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENN
                     PWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGTGG
                     LAVSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLP
                     ADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPG
                     VFTASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPK
                     DSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQAN
                     EDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFERFAHDPMAGGHRMWQM
                     AGLKAQRAQTDVNNKQAAFDAAAKEKADADAALSAAQERRKQKENKEKDAKDKLDKES
                     KRNKPGKATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRKKFWEEV
                     SKDPDLSKQFKGSNKTNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMNNI
                     RVTTPKRHIDIHRGK"
BASE COUNT         1732 a         1556 c         1900 g         1556 t
ORIGIN      
        1 aaaaaaaata tgtagagctg aaggggctac tgaagaggat gacaataaat tagtgagaga
       61 gtttgagcga ttaactgagc acccagatgg ttcagatctg atttattatc ctcgcgatga
      121 cagggaagat agtcctgaag ggattgtcaa ggaaattaaa gaatggcgag ctgctaacgg
      181 taagtcagga tttaaacagg gctgaaatat gaatgccggt tgtttatgga tgaatggctg
      241 gcattctttc acaacaagga gtcgttatga aaaaaataac agggattatt ttattgcttc
      301 ttgcagtcat tattctgtct gcatgtcagg caaactatat ccgggatgtt cagggcggga
      361 ccgtatctcc gtcatcaaca gctgaagtga ccggattagc aacgcagtaa cccgaaatcc
      421 tctttgacaa aaacaaagcg tgtcaggctg attctgatgc gctttttttt gaaatgtcac
      481 aaaaattcca tgtgggagat gggatctaaa atccccgtgc agaactttcc atccaggggg
      541 agaaaacttg tcgttttgag ccgttcggtg ttcagaacgc acgaaaccga tcgcgcatcg
      601 ctttcgtgca tagttatgca acgcgctaaa aacgattctg acgcattttt tatgtttacc
      661 ctggtgtttc cttgtctttt tgcgtttttt gcgtcagaaa gcgattgagg gcgttttaag
      721 gggtacgtac aacgggagtt atggtaaatg gatcgggttt tcgggaaggt tcgacaggat
      781 ttgccgttgg gtgtagtgta agcgactgaa aaacaaacgc ccctgaaatt cgtgtctctc
      841 cgccaagatt gtcacgagaa tacaggggcg aggtcggtcg ataacctgga aggaatcgta
      901 acacatgagc gccgcgcttc aatacttcga agaaaattta ccccaccgtc cctatcacac
      961 ggatgatctc gcttttggtc ttcgcatctc cggcaaaggg cgtgcgcttc ttgcgcggta
     1021 catccagcag aaccagcctc atgcgcagtt ctggctggtt tttgacgttg accgcgaggg
     1081 ggctgcgatt gactggagcg accggaacgc ccccgcgccg aacatcaccg taaaaaatcc
     1141 cgtgaacggt catgctcacc tgctctatgc actcaacatc gccgtgagaa ccgcgcctga
     1201 tgcgtcggtt aaggcactga agtacgctgc cgcgatagag cgtgcgctgt gtgagaaact
     1261 tggtgcagat gtgaactaca gcggactgat ttgcaaaaat ccgttccacc tggaatggct
     1321 ggtgatggag tggcgcgagg aagcctatac cctcgatgaa ctggctgatt atctcgattt
     1381 gagcgcctca gcgcgccgta gcatcgataa acattacggg atggggcgaa actgctacct
     1441 gttcgaaatg acgcgcaaat gggcttacag ggcgattcgt cagggctggc ctgtattctc
     1501 acaatggctt gatgccgtga tccagcgtgt cgaaatgtac aacgcatctc ttcccgttcc
     1561 gctttctccg gctgaatgtc gggctattgg caagagcata gcgaaataca cgcacaggaa
     1621 ctttacgccg gaaactttcg cacagtatgt ggctgatacg cacacgccag aaattcaggc
     1681 tacacgcggt cgcaagggcg gttctaagtc taagcgcggc acagtagcta catcagcacg
     1741 cacgctgaaa ccttgggaga aattagggat cagtcgcgcc tggtattacc aactgaaaaa
     1801 acgaggtctc gtagagtaga ccaaataagc ctatatcaga taacagcgcc tttttggcgc
     1861 ttttttgagc agcttggttt gttgctattt ccctcgttga atcccgcaat ggcgcggctt
     1921 tccgcatgat tgaggtggta gcgctcgccg cagtctcatg accgagcgta gcgagcgaat
     1981 gagcgaggaa gcgcaaaggt gtcggtggtg catgtggcac ttacgcgccg gggtttctcc
     2041 gttctacggt ttcgcaggtg gtttgggtag cttctccagc tcgttaatca gcggttgtat
     2101 ccggttcaca tcggcctgtc ttgtgacttc ctttcgcagg aactggagca ggaacgcacg
     2161 cagttgcgct tcttccggcc tccgtaccct cgccagcatg gcagccccca caatgacttt
     2221 ttgcgccgtg tccaggctcc ggctcttcgc cttcaggcgc tgtaatctgg cctcagcttc
     2281 ggcaatcttc tgttcgagtg ttctgctcat ttcgtgactc cgtgcgcggt gaaaaatcgc
     2341 attttagcgc gtcactggta gttgcaaaac tgaactggca taatgcacgg cacatcacga
     2401 agtgcgcact tatacaatct ccacttcgtt tcgattgtgt gcggtctgcg acgctaaaag
     2461 aaaacggcaa aaaggcatta cggcagaaat ggcgatttat catctcagca tgaaaatcat
     2521 ttcgcgaaaa aacggctaca gtgccgttgc ttctgctgcc taccgttccg gctctgtcat
     2581 acccgatgac cgtaccggat taatccacga ttacacccgt aaacgcggcg ttgatgatgc
     2641 ggtcattctc acccctgcga atgtaccgtc ctggtgtagt gaccgttccg ttctctggaa
     2701 tgcggtcgag aaagccgaac agcgccggaa ctcccagctg gcaagggaga ttgaactcgc
     2761 cattccccgt gagatttccc gcgaggccgc acgggaggcc gttctcgctt ttgtccggga
     2821 aaactttgtc agtcggggca tgattgccga tgtggcgttt catcacatgg accggaccaa
     2881 tccccatgcg cacatcatgc tgaccacgag agctgtcggg gaaacaggat ttgcaggaaa
     2941 ggtcagggac tggaacgagc gggcactcgc cgagacgtgg cgcgcatcat gggctgacca
     3001 tgcgaacaga gcgcttgcga acgccggcta ccaggaagag atagaccatc gttcatacga
     3061 gcgtcaggga ctggagaaag cgccgggcct tcacctcgga aaggctgcct gtgcgatgga
     3121 aaaacggggc atggaaacag aacgcggtga gcagaaccgc ctgattaaca gccttaacct
     3181 ggaaatacag gtttcccgca caaggctggc agtcatccag gtacaggaga acgtgcggaa
     3241 acgggaactc agcgatgccg cccgtcgtgc agcggaagcc cttaacctga ccattcccgc
     3301 tgcgaatgcc tcagcggata ccctgcggga attcattgcc acgctgccgc aggaatgcgg
     3361 gaacgcgtgg gagatgaccc cggagttcct ggcgatgagc gggaaggtga acgacatcga
     3421 acgtgagggg aatgcgctgc tgaaagagca ggccattctc gaaaaggaga tgaccggact
     3481 caaaaaagca cgccctgtcg cgtcccttct gtcagagatt cccctgatga catgggctga
     3541 accggaatac cgcaaaagac aactccgttt ctggaaactc gggaaacaga ttgaatctct
     3601 tcgccgcacc tacagggccg tgaaagaacg ggacattccc gcccgtcgtc aggcctttga
     3661 aacgcagtgg aatacgtgga ttgcgccggg aatggcagag ctgaaagaaa aactgtcagc
     3721 acgggaagcg gagcggcgca gggaggaagc cgaagcggaa gcgcgccgga aggaacagga
     3781 gcatgaggcg cggctgaaac gtcatgataa ccaccgtctg agccgtgaaa cggcattagt
     3841 cggggttatt acggagctgg ggcgtgccag agagccggga acgggcagga taacccgcta
     3901 catgatgttg agtaacagag ccggagaatt cacggtatgg ggtgatgagc tggcgcatta
     3961 cccccagagt gttcatgacc cggtgaatgt ttacctgtcg ccaggcgggg ctgtgatggt
     4021 ctcggatata cgcgagggaa tgccagaatc tcatgagacg atggcgcggc ctgagcgtgt
     4081 gagaatgtat tccggtgcga cggtccggca tgtactggaa cagatgcgcc aggggtggcc
     4141 ctcttacggt tttccggcgc tgccgcatca ctggccggat aatttttatt tcagcgacga
     4201 ccgcaggccc gtagcctctc cgctgccgtc agcgcaccgg gtggatatca ccgctgatgc
     4261 tgtaccggaa caactcatgc ctgttgtatt ttcgagagag ggaaacagca ggacgctgaa
     4321 tctgttgtta tgcaaagggc cggaggaaat gcttgtcggg tttgttcgcc gggaaggagg
     4381 gttttgcccc gttcttgtgc ttccgtcgcc ggattacagt catctgatag tcagcaccat
     4441 cacggagaac ggggtatgcc tggcaggtta cggagaagct ataaaccatg atgaggatac
     4501 tccgtaccca ccggaaccgc acctgatgca gttccggctc aaaggccatc atgacaggct
     4561 tttggctgct gtccataaac cggaagagat gccggattat ctcttccgtc aactcggttt
     4621 taatcagacc tggcatgagt ggaagcggga cgaacagcac aggcaacaac aacgccgccc
     4681 cgggcacttc cggggcatga gtatgtgata tccggggctg caccccggac cccgccaaca
     4741 catcacgggc cacaaaattt tttgtggccc gctctgcgtt ttctaagtgt tatccctcct
     4801 gatttctaaa aaattttccg cctgaacttg acagaaaaaa cgatgacgag tactttttga
     4861 tctgtacata aaaccagtgg ttttatgtac agtattaatc atgtaattaa ttgttttaac
     4921 gcttaaaaga gggaattttt atgagcggtg gcgatggacg cggccataac acgggcgcgc
     4981 atagcacaag tggtaacatt aatggtggcc cgaccgggct tggtgtaggt ggtggtgctt
     5041 ctgatggctc cggatggagt tcggaaaata acccgtgggg tggtggttcc ggtagcggca
     5101 ttcactgggg tggtggttcc ggtcatggta atggcggggg gaatggtaat tccggtggtg
     5161 gttcgggaac aggcggtaat ctgtcagcag tagctgcgcc agtggcattt ggttttccgg
     5221 cactttccac tcctggaact ggcggtctgg cggtcagtat ttcagcggga gcattatcgg
     5281 cagctattgc tgatattatg gctgccctga aaggaccgtt taaatttggt ctttgggggg
     5341 tggctttata tggtgtactg ccatcacaaa tagcgaaaga tgaccccaat atgatgtcaa
     5401 agattgtgac gtcattaccc gcagatgata ttactgaatc acctgtcagt tcattacctc
     5461 tcgataaggc aacagtaaac gtaaatgttc gtgttgttga tgatgtaaaa gacgaacgac
     5521 agaatatttc ggttgtttca ggtgttccga tgagtgttcc ggtggttgat gcaaaaccta
     5581 ccgaacgtcc aggtgttttt acggcatcaa ttccaggtgc acctgttctg aatatttcag
     5641 ttaataacag tacgccagca gtacagacat taagcccagg tgttacaaat aatactgata
     5701 aggatgttcg cccggcagga tttactcagg gtggtaatac cagggatgca gttattcgat
     5761 tcccgaagga cagcggtcat aatgccgtat atgtttcagt gagtgatgtt cttagtcctg
     5821 accaggtaaa acaacgtcag gatgaagaaa atcgccgtca gcaggaatgg gatgctacgc
     5881 atccggttga agcggctgag cgaaattatg aacgcgcgcg tgcagagctg aatcaggcaa
     5941 atgaagatgt tgccagaaat caggagcgac aggccaaagc tgttcaggtt tataattcgc
     6001 gtaaaagcga acttgatgca gcgaataaaa ctcttgctga tgcaatagct gaaataaaac
     6061 aatttgaacg atttgcacat gacccaatgg ctggcggtca cagaatgtgg caaatggccg
     6121 ggcttaaagc tcagcgggcg cagacggatg taaataataa gcaggctgca tttgatgctg
     6181 ctgcaaaaga gaaggcagat gctgatgctg cattaagtgc cgcgcaggag cgccgcaaac
     6241 agaaggaaaa taaagaaaag gacgctaagg ataaattaga taaggagagt aaacggaata
     6301 agccagggaa ggcgacaggt aaaggtaaac cagttggtga taaatggctg gatgatgcag
     6361 gtaaagattc aggagcgcca attccagatc gcattgctga taagttgcgt gataaagaat
     6421 ttaaaaactt tgacgatttc cggaagaaat tctgggaaga agtgtcaaaa gatcccgatc
     6481 ttagtaagca atttaaaggc agtaataaga cgaacattca aaagggaaaa gcaccttttg
     6541 caaggaagaa agaccaagta ggtggtaggg aacgctttga attacatcat gataaaccaa
     6601 tcagtcagga tggtggtgtc tatgatatga ataatatcag agtgaccaca cctaagcgac
     6661 atattgatat tcatcggggt aagtaaaaat ggaactgaaa catagtatta gtgattatac
     6721 cgaggctgaa tttctggagt ttgt
//
LOCUS       DAZGSP010000185         6686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZGSP010000185 DAZGSP010000000
VERSION     DAZGSP010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.186"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1244
                     /locus_tag="ACHK74_004916"
     CDS             <1..1244
                     /locus_tag="ACHK74_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP0223590.1"
                     /translation="WCSIVYLLNNMTVNDDANDVAVTESYKPVWDWKISQFNVSDIKF
                     ETMINPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFATGHLRIMAEGEN
                     FLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDIVLESLQPYLVINKDAVT
                     PCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNCNGNYMQNFTRM
                     KPLDEDTYAGIKDYPVCFYDPKYKIMANHHGITFDEQRKRYIIGPITVSSDEMTNALD
                     PVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGI
                     NQVVAHNGDGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSY
                     QHEKNRNGLMPLIRRRTMLQL"
     gene            1434..1547
                     /gene="yhiY"
                     /locus_tag="ACHK74_004917"
     CDS             1434..1547
                     /gene="yhiY"
                     /locus_tag="ACHK74_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFP0223591.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1610..2677
                     /gene="yhiI"
                     /locus_tag="ACHK74_004918"
     CDS             1610..2677
                     /gene="yhiI"
                     /locus_tag="ACHK74_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP0223592.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQISASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2674..5409
                     /gene="rbbA"
                     /locus_tag="ACHK74_004919"
     CDS             2674..5409
                     /gene="rbbA"
                     /locus_tag="ACHK74_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFP0223593.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDITHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5409..6533
                     /gene="yhhJ"
                     /locus_tag="ACHK74_004920"
     CDS             5409..6533
                     /gene="yhhJ"
                     /locus_tag="ACHK74_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0223594.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
BASE COUNT         1578 a         1650 c         1849 g         1609 t
ORIGIN      
        1 attggtgcag cattgtttat ttacttaata atatgacagt aaatgatgat gctaatgatg
       61 ttgcagttac tgagtcgtac aaacccgtct gggactggaa aatatcgcaa ttcaatgtat
      121 ctgatattaa attcgaaaca atgataaacc cccagttcgc tgacagaacc tatttctcca
      181 attgttcacc tgttgatccc acaagtacca gaccaaccta ttttggcgat accgatggct
      241 cagtcggtgc agttttgtac gcgctttttg caacgggaca tttgagaatc atggctgaag
      301 gagaaaattt ccttagccag ttgcttaata tagaagatga agttctcaac gtattgctaa
      361 gggagaactt taatgagcaa cttgacacta atgttaatac aataatcagt attctgaaca
      421 gaagagatat agttctagaa agtcttcagc cttatttagt tattaataaa gacgctgtga
      481 cgccatgtac gtttcttggc gaccaaaccg gcgatcgatt tagtaatatc tgtggcgatc
      541 aatttatcat cgatttgtta aaacgcataa tgagtatcaa tgaaaatgtt catgtattag
      601 ctggaaatca tgaaacgaat tgcaatggta actatatgca gaacttcacg cgtatgaaac
      661 cacttgatga ggatacctac gctgggataa aagattatcc tgtatgtttc tatgacccca
      721 aatacaaaat aatggcaaat catcatggga taacttttga tgagcagcga aaacgttaca
      781 ttataggacc gataacagtc tctagtgatg aaatgaccaa tgctctcgat ccggtcgaat
      841 tagcggcaat tatcaataaa aagcaccatg ctgtaattaa tggcaaaaag tttaaaaata
      901 gccgggctat ctcttgtcga tcctttaatc gttatttttc tgtttcaaca gattacagac
      961 ctaaacttga agcactatta gcatgttcac aaatgttagg tataaatcaa gttgttgcac
     1021 ataacggaga tggtggaaga gaacgcatag gcgaaacggg gacagtttta ggtcttaacg
     1081 cccgtgacag taaacatgct ggaagaatgt tcagcatgca taattgccaa attaaccctg
     1141 gtgcagggcc tgagataacc actccctgga agtcttacca gcatgaaaaa aacagaaatg
     1201 gacttatgcc tttgattaga aggcgcacaa tgttacagct ctgaaccata gacaacatga
     1261 atattaatgt tactatctgg ttgagactgt tgataaaacg taaaaaggaa tgctctgttc
     1321 ctgcaactga acaggcgtga aacgctatcc aacaggatgg ataccgtttt gtcagccagt
     1381 tttatagatc acacttatga gtatgcgtgt ttcatttacg gccaggaatg ctgatgatga
     1441 caacattact acccgttttc actaagcctt ccccgttggc gctcaatgct ctgcgcgctg
     1501 gtcgtatttg tcgtttcctt cttatcccgg atggaagaat ccgctgaacg tcgttcggcg
     1561 gcagttgtaa cacgcgtcgt ttgcttacag ggattatggc gggtcgatta tggataagag
     1621 taagcgccat ctggcgtggt gggttgtcgg gttactggcg gtggcggcta tcgtggcgtg
     1681 gtggctgttg cgcccggcag gtgtgccgga aggctttgct gtcagtaatg ggcgcattga
     1741 agcgacggaa gtggatattg ccagcaaaat tgccgggcgt atcgacacca ttctggtgaa
     1801 agaaggccag tttgttcgcg aaggtgaagt gctggcgaag atggatactc gcgtgttgca
     1861 ggaacagcga ctggaagcca tcgcgcaaat caaagaggca caaagcgccg ttgctgccgc
     1921 gcaggctttg ctggagcaac gacaaagcga aactcgtgcc gcacagtcgc tggttaatca
     1981 acgccaggca gaactggact ccgtagcaaa acgtcatacg cgttcccgtt cactggccca
     2041 acgaggggct atttctgcgc aacagctgga tgacgatcgc gccgccgctg agagcgcccg
     2101 agctgcgctg gaatcggcga aagctcagat atcggcttct aaagcggcta tagaagcggc
     2161 acgcaccaat atcattcagg cgcaaacccg cgtcgaagcg gcacaagcca ctgaacggcg
     2221 cattgccgca gatatcgatg acagcgaact gaaagccccg cgtgacggac gcgtgcagta
     2281 tcgggttgcc gagccaggcg aagtgctggc ggcaggcggt cgggtgctga atatggtcga
     2341 tctcagcgac gtctatatga ctttcttcct gccaaccgaa caggcgggca cgctgaaact
     2401 gggcggtgaa gcccggctga tcctcgatgc cgcgccagat ctgcgtattc cggcgaccat
     2461 cagttttgtc gccagtgtcg cccagttcac gccaaaaacc gtcgaaacca gcgatgaacg
     2521 gctgaaactg atgttccgcg tcaaagcgcg tatcccaccg gaattactcc agcagcatct
     2581 ggaatatgtc aaaaccggtt tgccgggcgt agcgtgggtg cgggtgaatg aagaacttcc
     2641 gtggcctgac gacctcgtgg tgaggttgcc gcaatgacgc atctggaact ggttcccgtc
     2701 ccgcctgtcg cgcaactggc gggcgtgagc cagcattatg gaaaaaccgt tgcgctgaac
     2761 aatatcactc tcgatattcc ggcccgctgt atggtcgggc tgattggccc ggacggcgtc
     2821 gggaagtcga gcttgttgtc gttgatttcc ggtgcccgcg tcattgaaca gggcaatgtg
     2881 atggtgctgg gcggcgatat gcgcgacccg aagcatcgcc gcgacgtctg cccgcgcatc
     2941 gcctggatgc cgcaggggct gggcaaaaat ctctaccaca ccttgtcggt gtatgaaaac
     3001 gtcgattttt tcgctcgcct gttcggtcac gacaaagcgg agcgggaagt gcgaattaat
     3061 gagctgctga ccagcaccgg gttagcaccg tttcgcgatc gtccggcagg gaaactctcc
     3121 ggcgggatga agcaaaaact ggggctgtgc tgtgcgttaa tccacgaccc ggaactgttg
     3181 attcttgatg agccaacaac gggggttgac ccgctctccc gcgcccagtt ctgggatctg
     3241 atcgacagta ttcgccagcg gcagagcaat atgagcgtgc tggttgccac cgcctatatg
     3301 gaagaggccg aacgcttcga ctggttggtg gcgatgaatg ccggagaagt gctggcaacc
     3361 ggcagcgccg aagagttgcg gcagcaaacg caaagcgcca cgctggaaga agcatttata
     3421 aatctgttac cgcaagcaca acgccaggcg catcaggcgg tagtgatccc accgtatcaa
     3481 cctgaaaacg cagagattgc catcgaagcg cgcgatctga ccatgcgttt tggttccttc
     3541 gttgccgttg atcacgtcaa tttccgcatt ccacgcgggg agatttttgg attccttggt
     3601 tcgaacggct gcggtaaatc caccaccatg aaaatgctta ccggactgct gcccgccagc
     3661 gaaggtgagg cgtggctgtt cgggcaaccg gttgatccaa aagatatcga tacccgccgt
     3721 cgggtaggct atatgtcgca ggcgttttcg ctctataacg aactcaccgt gcggcaaaac
     3781 cttgagttac atgcccgttt gtttcacatc ccggaagcgg aaattcccgc gcgagtggct
     3841 gaaatgagcg agcgttttaa gctcaacgac gttgaagatg ttctgccgga gtcattgccg
     3901 ctcggcattc gccagcggct ttcgctggcg gtggcggtga ttcatcgccc ggagatgtta
     3961 atcctcgatg aacctacttc tggcgtcgat ccggtggcga gggatatgtt ctggcagttg
     4021 atggtcgatc tctcgcgcca ggacaaagtg actatcttca tctccaccca ctttatgaac
     4081 gaagcggaac gttgcgaccg catctcactg atgcacgccg gaaaagtgct tgccagcggt
     4141 acaccgcagg aactggttga gaaacgcgga gccgccagtc tggaagaggc atttatcgcc
     4201 tatttgcagg aagcggcagg gcagagcaac gaagccgaag cgccgcccgt ggtacacgac
     4261 atcacccacg cgccgcgtca gggatttagc ctgcgccgtc tgtttagcta cagccgccgc
     4321 gaagcactgg aactgcgccg cgatccggtc cgttcgacgc tggcgctgat gggaacggtg
     4381 atcctgatgc tgataatggg ttacggcatc agtatggatg tggaaaacct gcgctttgcg
     4441 gtgctcgacc gcgaccagac cgtcagtagc caggcgtgga cgctcaatct ctccggttcc
     4501 cgttacttta tcgaacagcc gccgctcacc agttatgacg agcttgatcg tcggatgcgt
     4561 gcgggcgata tcacggtggc aattgagatc cctcccaatt tcgggcgcga tatcgcgcgt
     4621 ggtacgcctg tggaactcgg cgtctggatt gacggagcga tgcccagtcg tgccgaaacg
     4681 gtaaaaggtt acgtgcaggc aatgcaccag agctggttac aggatgtggc gagccgacaa
     4741 tccacacccg ccagccaaag cgggctgatg aacattgaga cgcgttatcg ctataacccg
     4801 gacgtaaaaa gcctgccagc gattgttccg gcggtgatcc cgcttctgct gatgatgatc
     4861 ccgtcaatgc taagcgccct tagcgtggtg cgggaaaaag agcttgggtc gattatcaac
     4921 ctttacgtga cccccaccac gcgtagtgaa tttttgcttg gtaaacagtt gccatacatc
     4981 gcgctgggga tgctgaactt tttcctgctc tgcggcctgt cggtgtttgt gtttggcgta
     5041 ccgcataaag gcagtttcct gacgctcacc ctggcggcgc tgctgtatat catcattgcc
     5101 accggaatgg ggctgctgat ctccaccttt atgaaaagcc agattgccgc cattttcgga
     5161 acggcgatta tcacgttgat cccggcgaca cagttttccg ggatgatcga tccggtagct
     5221 tcgctggaag ggcctggacg ttggatcggc gaggtttacc cgaccagtca ttttctgact
     5281 atcgcccgcg ggacgttctc gaaagcgctg gatctgactg atttgtggca actttttatc
     5341 ccgttgctga tagccatccc gctggtgatg ggcttaagta tcctgctgct gaaaaaacag
     5401 gagggatgat gcgccattta cgcaatattt ttaatctggg tatcaaagag ttgcgcagtc
     5461 tgctcggtga taaagcgatg ctgacgctga ttgtcttctc gtttacggtg tcggtgtatt
     5521 cgtcagcgac cgttacgcca ggatcgttga acctcgcgcc gatcgccatt gccgatatgg
     5581 atcaatcgca gttatcgaac cggatcgtta acagcttcta tcgtccgtgg tttttgccac
     5641 cggagatgat caccgccgat gagatggatg ccggactgga cgccggacgc tataccttcg
     5701 cgataaatat tccgcctaat tttcagcgtg atgtcctcgc cggacgccag ccagatattc
     5761 aggtgaacgt cgatgccacg cgcatgagcc aggcatttac cggcaatggg tatatccaga
     5821 atattatcaa cggtgaagtg aacagctttg tcgcgcgcta ccgtgataac agcgaaccgt
     5881 tggtatcgct ggaaacccgg atgcgcttta acccgaacct tgatcccgcg tggtttggcg
     5941 gggtgatggc aatcatcaac aacattacca tgctggcgat tgtattgacc ggatcggcgc
     6001 tgatccgcga gcgtgaacac ggcacggtgg aacacttgtt ggtgatgccg ataacgccgt
     6061 ttgagatcat gatggcaaag gtctggtcga tggggctggt ggtgctggtg gtgtcgggat
     6121 tatcactggt gctgatggtg aaaggcgtgc tgggtgtacc gattgaaggc tcgatcccgc
     6181 tgtttatgct gggcgtggcg ctcagtctgt ttgccaccac gtcaatcggc atttttatgg
     6241 ggacgatagc gcgttcaatg ccgcaactgg ggctgctggt gattctggtg ctgctaccgt
     6301 tgcaaatgct ttccggtgga tccacgccgc gcgaaagtat gccgcagatg gtgcaggaca
     6361 ttatgctgac catgccgacg acacactttg ttagcctcgc gcaggccatc ctctaccggg
     6421 gtgccggatt cgaaatcgtc tggccgcagt tcctgacgct gatggcaatt ggcggcgcgt
     6481 ttttcaccat tgcactgctg cgattcagga agacgattgg gacaatggcg taaatacaat
     6541 tgcccgatgc gtaagcttat agggactacg catcggtgca atttattgaa ttttagttgt
     6601 ttttaggccg gataaggcgt ttatgaactg acccccaaaa cctggacagt ttcagttaac
     6661 cggctttttg atactcagtc ctgtat
//
LOCUS       DAZGSP010000186         6641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZGSP010000186 DAZGSP010000000
VERSION     DAZGSP010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.187"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            191..754
                     /locus_tag="ACHK74_004921"
     CDS             191..754
                     /locus_tag="ACHK74_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0223595.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            836..3481
                     /gene="yfcU"
                     /locus_tag="ACHK74_004922"
                     /pseudo
     CDS             836..3481
                     /gene="yfcU"
                     /locus_tag="ACHK74_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            3501..4253
                     /locus_tag="ACHK74_004923"
     CDS             3501..4253
                     /locus_tag="ACHK74_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0223596.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTPYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            4228..4776
                     /locus_tag="ACHK74_004924"
     CDS             4228..4776
                     /locus_tag="ACHK74_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0223597.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAIDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            4773..5243
                     /locus_tag="ACHK74_004925"
     CDS             4773..5243
                     /locus_tag="ACHK74_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0223598.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            5240..5764
                     /locus_tag="ACHK74_004926"
     CDS             5240..5764
                     /locus_tag="ACHK74_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0223599.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            5751..6623
                     /locus_tag="ACHK74_004927"
     CDS             5751..6623
                     /locus_tag="ACHK74_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFP0223600.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
BASE COUNT         1759 a         1488 c         1682 g         1712 t
ORIGIN      
        1 taaaattaaa ttgcatcagg ggtgtggaaa catcactatg cggccccaca ccaacgaata
       61 caattttcta cgacctgttc tttttggatc gcatgccgta ttcaatggct gggtataaac
      121 attgggtatt gactgaatca atgtattgta atgtttatat ttttaattat ttatagttta
      181 aggacaaata atgagtaagt ttgttaaaac agctattgct gcggcaatgg tgatgggcgt
      241 tttcacctct acggcaacaa tcgctgcggg taacaacggt actgcacgtt tttacggcac
      301 cattgaagac tcagtgtgtt ctatcgttcc ggacgatcac aaactggaag ttgatatggg
      361 tgatatcggt gccgaaaaac tgaaaaataa cggcaccacc acgccgaaaa gtttccagat
      421 tcgtctgcaa gattgcgtat ttgatactca ggaaacaatg accactacct ttactggtac
      481 cgtttcttct gcaaatagcg gcaattatta caccattttc aataccgata ccggtgcggc
      541 atttaacaat gttagcctgg cgatcggtga ctctctgggt acctcttaca aaagcggcat
      601 gggtattgac cagaaaatag tgaaagacac ttctaccaat aaaggcaaag cgaagcagac
      661 actgaacttt aaagcctggc tggtcggcgc agctgatgcg ccagatctgg gtaatttcga
      721 agcaaatacc accttccaga ttacttacct gtaaggcata cccccatagc gacactgcta
      781 tgggggattt aaatggattt tgaccgaaca agaataaaaa cggatagaaa cgtgtatgcc
      841 tgaccattct ctttttcgat tacggattct tccgtggtgc attgcgctgg caatgtcagg
      901 gagttatagc agtgtctggg ctgaagacga cattcagttt gattcccgtt ttctggaatt
      961 aaaaggcgac acaaaaattg atctgaagcg tttttccagc cagggatatg ttgagcccgg
     1021 aaaatacaat ttacaggttc aactaaataa acagccattg gcggaagagt acgatattta
     1081 ctggtatgct ggtgaagatg acgcgagcaa aagctatgct tgtctgacac cggaactggt
     1141 agcgcagttt ggtttaaaag aagacgtggc gaaaaatctt caatggagcc acgatgctaa
     1201 atgcctgaaa tccggtcaac tggaaggcat ggaaattaag gctgatttaa gccagtccgc
     1261 attagtcatt tcactaccgc aggcttacct cgagtatacc tggcccgact gggatccgcc
     1321 ttcacgttgg gatgacggca tttccgggat cgtcgcggac tacagcatca acgcacaaac
     1381 ccggcatgaa gaaaatggcg gtgatgatag taacgagatc agcggcaacg ggacggtcgg
     1441 ggttaacctg gggccgtggc gtatgcgtgc tgactggcag actaactatc aacatactcg
     1501 cagtaatgat gatgacgatg aattcagcgg cgatgaaact caaaaaaaat gggagtggag
     1561 tcgctactat gcctggcggg cgttaccatc attaaaagcc aaactggcgc tgggcgagga
     1621 ttacctcaga tccgatattt ttgatggctt taactatgtt ggtggcagtg tcagtactga
     1681 cgatcaaatg ttgcctccca atctgcgcgg ctacgcgcca gacatttccg gcgtggcaca
     1741 caccacagca aaagtgaccg tcagccagat ggggcgtgtg atttacgaaa cgcaggttcc
     1801 ggctggaccg tttcgtattc aggatcttgg tgattctgtc tccggtacgt tgcatattcg
     1861 cattgaagaa cagaacggcc aggtgcagga atatgacatc agcaccgcct cgatgccata
     1921 cctcactcgc ccaggtcagg ttcgctataa gatcatgatg ggccgtccgc aagagtgggg
     1981 acaccatgtc gagggtgaat ttttttctgg tgctgaagct tcctggggga tcgctaacgg
     2041 ctggtcgtta tatggcggcg cactgggaga tgaaaactat cagtctgcgg cgcttggcgt
     2101 cggtcgcgat ttgtctacat tcggctcggt cgcgtttgat gttactcact cgcacaccaa
     2161 actggataaa gacaccgctt atggcaaagg ttcgctggac ggtaactcct tccgtgtgag
     2221 ttattccaaa gactttgacc agctcaacag ccgcgttact tttgctggat atcgcttctc
     2281 ggaagagaac tttatgacca tgagcgagta tctggatgcc agtgacggcg gaatggtacg
     2341 cacgggcaac gacaaagaga tgtacaccgc cacttataac tagaacttcc gcgatgcggg
     2401 tgtttcggtt tatctcaact atacccgcca tacctactgg gatcgcgagg agcagacaaa
     2461 ctacaacatc atgctctcgc actatttcaa tatgggcagt attcgtaatg tcagcatctc
     2521 gatgactggc taccgttacg agtatgacaa ccaggccgac aaaggcatgt acatttcgct
     2581 cagtatgccg tggggcgaca acagtaccgt tagctataac ggtaactatg gcagtgggac
     2641 ggacagcagt caggtcggtt atttcagccg tgtcgatgac gcgactcact atcagttgaa
     2701 cgtcggcacc agtgacaaac acaccagcgt tgacggctat tacagccatg atggttcgct
     2761 ggcgcaggtt gacctcagtg cgaactacca tgaagggcaa tacacctctg cgggcttgtc
     2821 gttacagggc ggcgcaacgc ttactgccca cggtggcgca cttcaccgta cccagaatat
     2881 gggcgagaca cgcttgttga ttgatgccga tggcgttgcc gatgttccgg tagaaggtaa
     2941 cggtgctgct gtttatacca atatgtttgg taaagccgtc gtttctgacg tcaataacta
     3001 ttaccgcaat caggcgtata tcgacctcaa caaactgccg gaaaacgccg aagcaaccca
     3061 gtcggtggta caagccacgc taactgaagg ggcgattggc taccgcaaat ttgccgtcat
     3121 cagtggtcaa aaagcgatgg cggtgctgcg tttacaagac ggcagccatc ctccgtttgg
     3181 cgcagaagta aaaaatgata acgagcagac agtgggcctt gtcgatgatg acggcaatgt
     3241 ttatctggct ggggtgaaac ctggcgaaca catgagtgtg ttctggagtg gtgttgcgca
     3301 ttgcgatatc aacctgccgg acccgctgcc tgccgatctg tttaacggct tgttactgcc
     3361 atgccagcat aaaggcaacg tcgcgccggt tgttcctgat gacataaagc ctgtcattca
     3421 ggagcagacg caacaggtga cacccaccaa tccccctgtt tctgtttcag ctaaccaata
     3481 acgtgattaa ggaatgatcc atgtctgatt tactttgctc tgccaaattg ggtgctacga
     3541 cgctagcgtt actgcttagc gctgccagtt tgtctgccca ggcttctgta acgccagatc
     3601 gtacccgttt gatatttaat gaaagcgaca aatcaatcag cgtgacgttg cgcaataacg
     3661 atccgaaact gccttatctg gcccaaagct ggatcgaaga tgaaaagggt aacaagatca
     3721 gttcaccgtt aactgttctg ccaccggtgc aacgcatcga ttcaatgatg aacgggcagg
     3781 taaaagtgca gggaatgccg gatatcaaca aattgcctgc cgatcgtgaa agcctgttct
     3841 acttcaacgt gcgtgagatc ccgccaaaat caaacaaagc caacacgctg caaatagcac
     3901 tacaaacgcg tattaagttg ttctggcgac cgaaggcgct ggagaacgtc agcatgaaaa
     3961 acccctggca atataaggtt actttaaccc gcaacggtca ggaatttacc gtcaataacc
     4021 cgacaccgta ttacgtcatt ttcagtaatg ccagcacaca gaaaaatggc aatccggcag
     4081 ccggtttctc gccaatagtg atgtcgccga aaacctctgc accgctgaat gtgaagatgg
     4141 gtagcgtgcc ggtactgacc tatgtcaacg actatggcgc tcggatgccg ctctttttca
     4201 cttgcaacgg taacagttgc caggtcgatg aagagcagag ccgcaaaggc tgaggccggg
     4261 agagccagta tgaaaggatc ttgttctgtc tgggcggtaa ctgggttact gatatgttgt
     4321 gttggtagcg gctttgcagt tgcagcgagt aagtcagtaa gtatgaattt acgggtgctt
     4381 gtcgatgccc ctcctccttg tacggtaaat ggtgcggcag ttgagtttgg taatgtattc
     4441 attaataaga tcaatggggt tgattataaa cgtcccattg actactcgct tgtttgtaac
     4501 aatctggcaa tagatgacct gcgtctacag atgcaggcga caacggtcgt gattaacggt
     4561 gaaacagtta tcagcacggg gattcctggg tttggtattc gggttcagaa aagtagtgat
     4621 cacacaatac tcgacttaac ctctggctca tggttgccat ttaatttttc ctcgggagtt
     4681 cccgttctgg aagctgtgcc ggtgaaacag agtggtacaa cgttagcggc agctgaattt
     4741 aacgccagtg ccacaattgt ggtggattac caatgaaacg gatctcgctt attttgctgt
     4801 ggggcttttg ctcgatggca ctctcaaacg tctcttttca cggttattta gtgcagccac
     4861 ctaactgcac gattagcaac gctcaaacca ttgaaataac ttttcaggat gtgcttattg
     4921 atgacattaa tggcagcaat tatgagcaaa ccgtgccgta ttccattacc tgcgatacgg
     4981 cggttcgcga cccattaatg gaaatgacct tgagctggag tggcacacca agcgattttg
     5041 ataatgctgc agtaagcagc aatatcactg gtttaggcat tcagctcaaa caggcagggc
     5101 agagttttac gatcaatacg ccacttgtgg tgaatgaaac cgatttaccg gtgttaacgg
     5161 ctgtgccagt aaaaaagagt ggtgtcgttc tgccagaggc ggactttgag gcctgggcca
     5221 cgttacaggt agattaccaa tgaagaaaaa gcgtacgttg ttttttatta gtagcctgat
     5281 gttgctgggc agtggaacca caatagcagg agataatttg cactttaccg gcaatttaat
     5341 cagtaaatcg tgtacgccag tgattaatgg tagccagttg gcagaagtac attttcctgc
     5401 cattgccgcc agcgatttaa tgaaccttgg gcagtcagag cgagtgccat tggtgtttca
     5461 attgaaagat tgtcatagtt ctacattgtt taatgtcaaa gtaacgttga caggaacaga
     5521 agatagtgct ctacccggct ttctggcctt tgactcctcg tcatcagcat ccggcgctgg
     5581 gattggtatt gaaactgcgg caggcacatc ggtgccaatc aacaatacaa caggcgtcac
     5641 gctcccgctg aatcagggaa ataacagcct aaattttaat acctggttac aggcaaagag
     5701 cgggcgagat gtaacgtccg gagactttag tgccacagtg acggcaacct ttgagtattt
     5761 ctgaaatgaa aaaatggacc ataattttcg cgagcctaat gctgctcgtt ctgagtgttg
     5821 tcggcgcatc taagagttat gctggagata aacttatgag tgcgagtttt gacagtgtga
     5881 gagtttatta tgccatggac aaggtaaccg gtgctgtagc gagcagtgtt ttcaatgtta
     5941 cggtgataac gccaaaggag gttgcatatg ggaaatatga ttcttttgcg tataaaggcg
     6001 atacgttgcg ggttatttcc tggtctgggt cgggttcagc tccaactttg gtactgactg
     6061 actttgatac aattaataac tcaaattgcc ctggcataga tacgaaaatt tttcgttgtg
     6121 catatatgac gtttaaaatt accgttgcat ctgatgatta tggttgccct tggatcgcat
     6181 ccttttattc ctatacagat ttacccggat ttggaagcta tacggcccct acagtgcata
     6241 acacaatatg cccaacgata ccggtcgctt catacgatat ctcctggagt gaaaattatg
     6301 tcagccataa taaggcgtta cgaattcagt ccacggggtc aacagtgacc accacgcttt
     6361 cgacctactt gatggaaggc ggcagactct gtgatggcag taatttttct gataatgatg
     6421 gcagaggagc atactgccgc gcggtatcag aattgttaac ctttacctct tacggttgtg
     6481 ataaatcaac ggtaacggtt acgccaactc gtcatccggt aacggataaa gtactacacg
     6541 atattgtggt gaatgtgaat accagtagtg ggcaaccgat tgattcgacg tgtcgttttc
     6601 agtatgtatt gaatgaactg tagttcgtag tattgctctg a
//
LOCUS       DAZGSP010000187         6497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZGSP010000187 DAZGSP010000000
VERSION     DAZGSP010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.188"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..123
                     /locus_tag="ACHK74_004928"
                     /pseudo
     CDS             1..123
                     /locus_tag="ACHK74_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(435..935)
                     /locus_tag="ACHK74_004929"
     CDS             complement(435..935)
                     /locus_tag="ACHK74_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP0223601.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDETDPELMFQDYEGFPGNMASECHINWAWVEGFRRARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(1400..1996)
                     /locus_tag="ACHK74_004930"
     CDS             complement(1400..1996)
                     /locus_tag="ACHK74_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223602.1"
                     /translation="MMKSDEQYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLAWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVSITDVLR"
     gene            complement(1993..2712)
                     /locus_tag="ACHK74_004931"
     CDS             complement(1993..2712)
                     /locus_tag="ACHK74_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFP0223603.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            complement(2709..3143)
                     /gene="psiB"
                     /locus_tag="ACHK74_004932"
     CDS             complement(2709..3143)
                     /gene="psiB"
                     /locus_tag="ACHK74_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFP0223604.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(3198..5156)
                     /locus_tag="ACHK74_004933"
     CDS             complement(3198..5156)
                     /locus_tag="ACHK74_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP0223605.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHTLPGERYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHIREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRTGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKQQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCTPRPQTDATEHTTNLADAA"
     gene            complement(5222..5455)
                     /locus_tag="ACHK74_004934"
     CDS             complement(5222..5455)
                     /locus_tag="ACHK74_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFP0223606.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEHFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            complement(5513..6040)
                     /locus_tag="ACHK74_004935"
     CDS             complement(5513..6040)
                     /locus_tag="ACHK74_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0223607.1"
                     /translation="MSARGINKVIVVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMRVQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQVQPQPQQNGQSQSADATKKGSAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            6377..>6497
                     /locus_tag="ACHK74_004936"
     CDS             6377..>6497
                     /locus_tag="ACHK74_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223608.1"
                     /translation="MRRSFLTHRQVCRERRSRRDRGGATGTVAASPGFHASDRS"
BASE COUNT         1371 a         2017 c         1760 g         1349 t
ORIGIN      
        1 ggaagggcag acaactgcgc catcaggcgc agtttcctta cacaccgtca ggtgtgcagg
       61 gagcgcaggt cacggcgcga cagaggcggc gcgaccggaa cggtcgctgc aggcccgggt
      121 tttcacgcat cggaccgtag cggcagcccg aaaccgcgcc agcgggttcg gcggagactg
      181 gcgggtgcgc cagcagctgc ctgtcggagt cgggcctgac gaccggatgc cgccaggcag
      241 ccgggagccg aaggtgtccc cgcccccggg agtcagaaca acagaaacga caaaacagaa
      301 aaatgcctca caccatgacg ggcaggaagg gcagcgccgc aggcgcggcc ctcctgtatt
      361 tcaggcaggc gtaagcctgc cagggttttc cggcggcccg gcaggccata cccccgccgg
      421 agcgggggag taaatcaccg acggaacaca tgaccgtcaa taaaggtgaa ggagtccagg
      481 aataaatccc gcgcatacgc ctcatagtca aaatagagcg ccaccgtctc cggcacgtca
      541 gccagcaggc cggtatcact gacgaactca accgcaaaag cctcctcact gtcagcctcg
      601 ccccaccagg catcgcggaa gctgtcaaaa tccgtctcac cggtatcatc cacccagaga
      661 cgataagcct cttcgcagcc ttcatcccgt gcccggcgga agccttcaac ccaggcccag
      721 ttgatatgac attcagaggc catattcccc gggaaaccct cataatcctg aaacatcagt
      781 tccggatcgg tttcatcctg gtgaagggca cggcaggcgg cgaaaaaatc gcgttcatca
      841 tcaaacgtgg tcaggtcaaa ccagcggccg gcgatgcttc cgcagttata tttgtgccag
      901 gttccaacgt atacagcagg tgcaacaaca gacattcctg aatcctccat agtcacatgt
      961 tctgcgacac cgggcggtgc cgtgcctcca tctttgccgg gcctgcaggg atgcaagggc
     1021 gcaagggaag ccgcagcccg acgcgcacgg ctgacgggag ttggagggtg agaccgcgaa
     1081 atctgagcgg gtgaacgacc ggaaaatcac ggcagacggg aagaagggac cgggtgaccg
     1141 cagcggttca tcttaccttg catcccgccc gcagaagccc ggctacgatg gaggcacggc
     1201 acagcccgca cacgacgctg aggccacata actgaaaacc cacaaggcca tgccccgcat
     1261 ggccgggtaa aagcggagcc gccggcgacg tccttattcc ggtgagagca gaggacacgc
     1321 cgcatgacct ccagaccacc cctgatatga aacgatcatg accggcatgt ccctgcccct
     1381 caagcatacc tccactggtt caccgaagaa catccgtgat gctcacaagt tttctgtcgc
     1441 gcagccagtc ctgcgcttca cgcaccacac aactgtccgg tttcaccgct ttcagagaag
     1501 atgccagtcg cttgatcaac atcaccagtt cccgtgtctg tatgtccggc attgtctccg
     1561 gcggagcaga gggactctca tttccgacgc ccatcccatg cgcttccagt gtggcacgga
     1621 caaaccaggc agcatcgacc agttgctggc gaatccaggc cagatcggca tttctgtcac
     1681 gggctgccat tcgctcaccc gacagcgcct tgctgatatg gctgcgcacc agattatcca
     1741 ggagttcctg cgcttcttcc ggtgaaaagc cacctggtat ctctgtgcgc aacagtcctt
     1801 tcctgtacag tagctgcagc agatcaatct gcgaccgcac tgcaactatc agtacataac
     1861 ggacaatatt cgcactggcg gtggtttctg tatcattcag cagttcctcc ggatcattac
     1921 caccggtatt gcagagcaac ggcaacagag gtcgcatcca cacgggaacc tgatattgtt
     1981 cgtctgattt catcatattg cgttctccag cttgttcggg atcatccact gctcaagtgc
     2041 acgtacctgt accggtgcac cacgaccggc ctcaccggct tcaaaaatca gccgccagag
     2101 tggttcctcc tgccagaaaa accggtcaaa tgatgacagt gatggaaaac gcccgcacca
     2161 cccccagaaa atcgtttcca gatcatgctc ctcaacaaca ccagaccagc gggacaacca
     2221 gcttccgacc gtggcgggag tatgaaacgc aaggtcaatc tgcgcctgag caaaacatgt
     2281 ctggtattcc agttcccgtc gttctgcctc acgttcccgc tggcggctgt atttctgctc
     2341 tgtcagcgtg gttttctgac gacgacgctc accttcacga aaaggaacgg acgggaacag
     2401 acgatgcccc gcatcggatg gcaggggaag aacacagact tcaccaaaac tttcaatcag
     2461 tttatccacg gcagccagcc acaggagacg gttgccatga aattcctcct tcgtcagcgc
     2521 aggggcaaaa aagcggagat cggtcagcga aatccggcga ctgccattca ggcagcgaaa
     2581 gaaagcgctg gcataaggcc atgctgaaag tgtgcgtccc tgacgacgac gttgttcggt
     2641 gacagccaca gcctgcatgg cagcctgttg ttctgcgctg agggggatca gtgcccgtga
     2701 acgtgcgctc atgcctgacc tccttctgcc atcaaaatat gaatgatgtc gttaacactg
     2761 tatccctgcg catcaagtgt cgcggcgagc gccagcgtgt gactgatcgc gacaggcgtg
     2821 aagatatgtt gcacctgaac gacggcgaaa ggctgtccac caccattcac cagaaccagc
     2881 atccagagtt gagagacgtc tcccggcgaa cataatgcca ggtggaaacg ttcgtgggca
     2941 ggcgtgaaac gaacctggac ggggaaaaag cccccgaact cactgccata ctcgccattc
     3001 atcatccagt tagccggtgc gtccacttca cgcatcacct cgtgagtgag attacggcgc
     3061 atatcgcttc ccgcagcacg gatatcttca tattcctgtg cgttcatgga ctgcagggca
     3121 ttcagggtca gttctgtttt catcacggta tctcccttgc tgccgccccg tctccggcgg
     3181 ggcggtgtgg ttgttcatca ggcggcatca gccaggttag tggtgtgttc agtggcatct
     3241 gtctgtggac gtggtgtaca catccagcca ggcacccagc ggttgtcttt catatggaac
     3301 tctgcatgtt cagccgcatc gcctttcttc atcttctccg cgtcccgtgc ggcaccggac
     3361 agtcctgcct cattcagggc tgcgataatc tgctgctttt tcaggtgtcc gaagaagttt
     3421 gcttttgtcg gctgccacca gtcgcgcatg tgaaaaccga tggcgctctc cagcgtgtcc
     3481 agcgggctgc gtgacgtgtg accacactcg cgggtctgaa cgccgtggat gctgcacgcg
     3541 gtgcagaaac tgagcaggga taacagcacc tcctgactga gtgaaaggaa cgtcgtcatg
     3601 tcccgtgacc atccctccgg taacagggcc gcaagacggg ctttttctgc catcatcgcc
     3661 atgaacgcgg caccttcctt ccccgatggc gcatcgctgg tcagcgaata atgtttacat
     3721 tccaggctga tttgtgctgg tttactgtac gctccgctgc caaacacatt caggcagagc
     3781 gtccatgcca gcagtgccag agatttgtcc ggttgctgca tgagtgccgc ctggactgcc
     3841 agcgtgcgtt cggaagacat tttcgtcagc agtggcagac tgatttcctc cactgatgct
     3901 ttctcctgca cctgttccgt gcggtcagcg tcgtcagccg cgtcatcttc actgcgcaac
     3961 tgcacaccac gctggacaca cacattaccg taacgccagg acaccaccac accgcttccg
     4021 gtacgcatct ccggcgtcca cgccctgacc ttcgccatgc aacgcatcag cttcatttct
     4081 gcttccagca ggtcggattc ctcacactgg ttttccagtg cgtcgtaacg tgtcatcagt
     4141 tcgttcaggc gttcttcttc tgcctccgtc agcaccgctt ccggctccgg cagacaacgg
     4201 tatgttccgg catcctcacg gcactcaccg acaggctcca tacgtccggc gcaccattcc
     4261 cagccttcgg cttcccgaat gtgttcagcg acagcctgga gtttttccag cagggcggca
     4321 tcgagcgcca cgcagtccac atagccaccc tcgtcgtcgc tgaacaggtc agtgcgcagt
     4381 tcatctggcg agaaggcatc agcccccacg aagcggaatt tactgttccc cgccaccgcc
     4441 acttcacttt cggtcaccag acgacgaatg gtctgcactt ccggtttacc gccccatcct
     4501 gactggcagg cggcttcaaa cacctgtacc tgacgcgcgg tgtcgttctc cagcgccagc
     4561 gcctgacagt gttctgtggt gatgcggtct tctgccagcg catcaaggat gacaggtgca
     4621 aggtctgcca gtttcagcat tcgctgaacg tggcggggtg aatagcccag caaatcaccg
     4681 atttgtgcag gtgttttgcc ttcctgcgcc attgcacgga atccggcaat ctgttcggca
     4741 gggtgcatat cccgacgatg accgttttca gtcattgatg cggcagtcgc cagttcctgc
     4801 ggaataattt tcacgcggac aggccagtca gccggaatga ttccacgctc tgccagcatg
     4861 ttgagtgctg ccagtctgcg accacctgcg gcgacaccgt agcgctcacc aggcagggta
     4921 tgcacgacca gattctgcaa caggccaacg cccttaatgg actcagccag ttcgctgacg
     4981 gactccgcag aatacggcac cgtgcgcaca ttcagtggtg acttaatcag cgaggccagc
     5041 ggcacgctca cttcctccgt ctgcgccagc agggcagaca ggactgtttc ctgcgttttt
     5101 gcaggtttac ggctggattt acgctccgtt tttgtcttag actctgtaac tgacatagtt
     5161 agtacctccc aaaacggtaa taatgattgt ctgccggacg cgttcccgtc cggcacctct
     5221 cttacttaag aatgccgaaa tcacggatga catggttcat ccagtacccg gcaccgtcct
     5281 caaaattcca ggtacgccag accattgttc cgttattacg gacaaccagg cgaaaatgtt
     5341 ctccgtggtc atcctcgata aagacgttcc ggtactgtgc ggcaacggct tcggcatgtt
     5401 tgcgggtaaa aggaccgtca ggcagcccct gaagtattct gaaatattcg ctcatcctca
     5461 tatctccggt gatgccccgc gcagggcggg gcggttatca cagtcaggcc agtcagaacg
     5521 ggatatcgtc tgaaaacccg taatcctccc cctccggcgg ttgcggctga ggctcgggct
     5581 gcgcggcctt acgtccacgg cctttcgttt tcgcgctccc ttttttcgtc gcgtcagcac
     5641 tctgtgactg accattctgc tgaggctgcg gttgcacctg agcgttctgc tgtggtgcac
     5701 tccccagcat ctgcatggtg cccgtggtct taacaagaat ttcagtgacg taacgggtga
     5761 taccgttatc ttcccagcta cgggttcgga gttgcccctc gatatagacc tgcgcgccct
     5821 tgcgcagata ttcacctgcc acttccgcga gcttgccgaa cagcaccacg cggtgccatt
     5881 ccgtctgcac ccgcatctcc cccgtctgtt tgtcgcgcca ggtttctgac gtggcgacct
     5941 gcaggtttgc cactgcgccc ccgttgggga tgtaacggac ttccggatct ttacccagac
     6001 gaccaaccac gatgaccttg ttgataccac gtgcactcat aaaaacctcc tgactgaaaa
     6061 gatgtgagat aaaccggata ccggcgacgg gacgttcatg tgccccttca gaggagcgaa
     6121 agctcctcct gccgtccact tcacaggggt ttctccccgc agtggacctt tccgatgggg
     6181 gcccgtttac cgcctcacgg gacagctgtc cgggggcgga cgcaaaattt tttgccgggt
     6241 tcatggctaa aaattttgtg gccggtgcat ctcaccctgg acagatggaa cgggaggggg
     6301 ttacgtctgc cccccggaaa gtccacagcg ggaaacaggc cgcagaggtg gcggcaggag
     6361 ggaagacagc tgcgccatca ggcgcagttt cctcacacac cgtcaggtgt gcagggagcg
     6421 caggtcacgg cgcgacagag gcggcgcgac cggaacggtc gctgcaagcc cgggttttca
     6481 cgcatcggac cgtagcg
//
LOCUS       DAZGSP010000188         6203 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZGSP010000188 DAZGSP010000000
VERSION     DAZGSP010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.189"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..76
                     /locus_tag="ACHK74_004937"
     CDS             <1..76
                     /locus_tag="ACHK74_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223609.1"
                     /translation="WAVARKREKHELELMIDEYLLYAC"
     gene            386..1545
                     /gene="istA"
                     /locus_tag="ACHK74_004938"
                     /pseudo
     CDS             386..1545
                     /gene="istA"
                     /locus_tag="ACHK74_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1751..2545
                     /gene="istB"
                     /locus_tag="ACHK74_004939"
     CDS             1751..2545
                     /gene="istB"
                     /locus_tag="ACHK74_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFP0223610.1"
                     /translation="MHELEVLLSRLKMEHLSYRVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPRVKTLEQFDFTFQPGIDRKVVRELAGLAFLERSEYVIL
                     LGPPGVGKTHLAIAPGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAILE
                     RLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
     gene            2656..2799
                     /locus_tag="ACHK74_004940"
                     /pseudo
     CDS             2656..2799
                     /locus_tag="ACHK74_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3968..4987
                     /locus_tag="ACHK74_004941"
     CDS             3968..4987
                     /locus_tag="ACHK74_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0223611.1"
                     /translation="MNNEQMLIKCRDPRKCRFYAEYLNHKYPNEFPHFAPDNPQNCIR
                     NVNLFLKEKYCNVSNPPFGTFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTLYLSKEDHTNFPEMNHNSSGIKNGIDVRKRRTVSDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNEKSIYLEELRQAWLKKKSTCQKFKWLLPDDDEMC
                     QWFWTRLKQKQDETSPVHKSVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNAKEMLDELRTVYGLRTGEFFEMLITEKYIE
                     HKRRK"
     gene            5097..5969
                     /locus_tag="ACHK74_004942"
     CDS             5097..5969
                     /locus_tag="ACHK74_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP0223612.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRQILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
BASE COUNT         1848 a         1448 c         1418 g         1489 t
ORIGIN      
        1 ctgggctgtg gcgcgcaaac gggaaaagca cgagctggaa ctgatgattg acgagtacct
       61 gttatacgcc tgttgattca taacgttccc ggggttcctt cctcatcgtt acacacagcc
      121 ggacggctat ctatggctgt ccgccaaatg accatcgccg catttcgttc tgctgaaaac
      181 ggtacgtagt cagtgatacc ggaatttccg gagaggatcg caggtatatt atttctgtgc
      241 aattcatggt tattcacatt ggatgtagag attgctgcat ccaatgttag cgccagtgat
      301 ataagacggt aattcaccat ttggattgtc cgctccaccc aaaatgttgt ttccttaagg
      361 ttctcacacc agaaaggaca tcaacatgct gagcagagag gacttttaca tgataaagca
      421 aatgcgccag cagggcgcgt acattgtcga tattgcgact cagattggtt gctctgaacg
      481 aacggtcaga cgttacctca aataccctga accgccagcc agaaaaaacg ccacaaaatg
      541 gttaagctga aaccgtttat ggattacatc gacatgcgcc tggcagagaa tgtccggaat
      601 agcgaggtta tcttcgcgga gattaaggca atgggtgata cgggcggacg ttccatgctg
      661 cgttactaca tccagcccaa acgtaaaatg cgtccgtcaa aaagaacagt tcgcttcgaa
      721 actcagcctg gataccagct ccagcacgac tggggcgaag ttgaggtgga gattgccggg
      781 caacggtgca aagttaactt tgcggttaat acgctggggt tctcccgccg cttccatgtc
      841 ttcgccgcac caaaacagga tgctgagcat acctacgaat cactggttcg cgccttccgc
      901 tacttcggtg gttgtgtgaa aacggtgctg gttgataacc agaaggctgc tgtgctgaag
      961 aataacaacg ggaaagtcgt gttcaactcc ggattcctgt tgctggccgc ccactataac
     1021 ttcctgccac aggcatgccg tccacgcagg gccagaacaa aaggtaaggt tgagcggatg
     1081 gtgaaatacc tcagggagaa cttcttcgtc cggtaccgca ggttcgacag cttcactcat
     1141 gtcaatcaac aactggagca atggatagcc gatgtggctg acaaacggga gcttcgccag
     1201 ttcaaagaaa cgccggaaca gcgcttcgcg ctggagcagg aacatctgca gccgttaccg
     1261 gatacggact ttgataccag ttacttcgac atccgccatg tgtcctggga cagctatatc
     1321 gaggttggcg gtaatcgtta cagcattccc gaagcgctgt gtggtcagcc ggtatcgata
     1381 cgaatatcgc tggatgacga gttacggctc tacagtaatg agaaactggt ggcctcacat
     1441 cgcctctgtt cggcatcgtc tggctggcag accgtgccgg agcatcacgc cccgctctgg
     1501 cagcaggtca gtcaggtgga acatcggcca ctgagaatga gatgaacgcc tcccaccatt
     1561 aaatccggag cacaaaggta gatggcggga aaatacagga aggtgtggag cctcgcagat
     1621 ttccggcatc atagtgccca atacgggatg cataagactc cctcagactg gagactccac
     1681 aaataaaata tacaccggtt ggcgttgata tcgcaaaaca cctgattcag gcctatgagg
     1741 aactgttgtg atgcatgaac tggaagtact gctgagtcgc ctgaaaatgg agcatctgag
     1801 ttatcgcgtt gaaagcctgc tggaacaggc agctaaaaaa gagctgaact accgggagtt
     1861 cctgtgcatg gcgctacagc aggaatggaa cggcaggcat cagcgcggta tggagtcccg
     1921 gctgaagcag gctcgtctgc cgcgggtcaa aacgctggag cagttcgact ttaccttcca
     1981 gccaggcatc gatcgtaagg tcgtccggga actggcaggt ctggcgttcc tggagcgcag
     2041 cgaatacgtg atcctgctgg gacctccggg tgtaggaaaa acgcatctgg ccatagctcc
     2101 tggcgtgaaa gcggtggatg cgggacatcg ggtactgttt atgccactgg acagactgat
     2161 cgcgacactg atgaaagcga aacaggaaaa ccggctggag cgtcagctac aacaactgag
     2221 ttatgcccgg gtgttgatcc tggatgaaat aggctatctg ccgatgaaca gagaagccag
     2281 cctgttcttc cggctactga accgtcgata tgaaaaagcg agcattatac tgacgtcaaa
     2341 caaagggttc gcagactggg gagaaatgtt cggagatcat gtgctggcaa cagcgatcct
     2401 ggagcggctg ctacatcact caaccacgct gaatatcaaa ggagagagtt accggttaaa
     2461 agagaaacgt aaagcaggag tgctgaccaa aaacacaacg ccaatcagtg atgatgaaat
     2521 ggtggaaagc ggacagcatc agtaacgaaa gtatcttagc gggcatgaaa atggcaaata
     2581 ggttaaaaat cgtggcgttg acaccaatgc tattggaaag agattaacca gcaaatggtt
     2641 cctcaaatcg tattgatatt tacccggcgt attgagcacc accatctgac actccgttcc
     2701 cgcatcaaac gactggaccg aaaaacgatt tacttttcac gtgctgtgga aattcacgaa
     2761 aaagttattg ggtcattcat cgaaaaacac atggtctgat gactggagtc atcctccaga
     2821 aatatacgtc aactgaacaa taacgatttt ttatgggatt ccatacactt ttccatattc
     2881 ctctgaaaat cagtcatcag ctcaccaaca cctcttctga aatttgtatt aaacttattg
     2941 cttaaataga tgatataaca tctcttccat tgcactgaat cagcgaaaac tgccgtttta
     3001 acaatcttaa taatatcatt tatcggttaa ctttatcagt aaacaatgga atatcacact
     3061 gatgaaatga acattcagag taaaatgata agatttacat aaacgccatt gctgaatatt
     3121 atttatttca tgcagataaa cgataaaata caaaatagta tatttcttaa tgtaaaagtc
     3181 aattattcat ctccactggc gagcacggtc agcacacaaa caaagtgagt cctcctgctt
     3241 aatcattgcg tatcagaaac taaaaaagaa aaaattctgc agacaactta acaatcttca
     3301 tcacatttcc tgaagacaga catatccaca aacggtagtc agcgttgaat taacaagtct
     3361 tacagagcta tacgtggctt ataaagccac tcaatgttca tcattacccg actacttatc
     3421 tggaatacca ccagtataaa ctcatcaata aaaacacctg ttgttaaaat aagaatactg
     3481 tcagcagtaa attattaaaa caacatctgt ccacactatc tgttctttta acgccaacgg
     3541 cagcgaaaat agtgcatcac agccttcttt tttctttcgc caccactcag acaaacacaa
     3601 cactcacacc cacaccataa aacagaatca aaacataaaa ttaacaataa cagtcaaaag
     3661 gaaaacacag aatattgagg tggactgtca ccaggacagc gcatacgacg catgtgatca
     3721 gcacatcccg ttcttcgctg gcatccccaa aatgcagaac aaacaatcat aaccttatga
     3781 atataaaaga aaatatgatt tttcaccatg aaaaaaacat caatgtatct ctgtataaaa
     3841 ataatgataa cattacataa atatttaaaa acataaataa acagcaacaa aaacatttga
     3901 taatcactta gtgattaaat acaattaaga cgattttgtt ataagagagg caccatggag
     3961 tgagataatg aataacgaac agatgcttat caaatgcaga gatccccgta aatgtcggtt
     4021 ttatgccgaa tatctgaatc acaaataccc taatgaattc cctcactttg cgcctgataa
     4081 cccacaaaac tgcatcagga atgttaattt atttttaaag gaaaagtatt gtaatgtttc
     4141 aaatcccccc tttggtactt ttggtactac tgaagaagaa gcactcaata acgaaattaa
     4201 tgagcttgaa attattgccg ggcaggcaat actgcctgaa gagagttttc tctggttgaa
     4261 aactgacgaa cttgcgactt tctttacctg gacaactctt tatctgtcga aggaagacca
     4321 tacaaatttc ccggaaatga accataattc atcaggaata aaaaacggaa ttgatgtcag
     4381 aaaaagacga acagttagcg atgaaacatt aaacacttac aaaaaaagca atcttccgga
     4441 taacccatca tcccataacg aacgaataaa agtaatcatt tcatattttg actcctgcca
     4501 ttctttaatt aataaaaatg aaaaaagcat atacctcgag gaactcaggc aggcgtggct
     4561 taaaaagaaa agcacctgtc aaaagtttaa gtggctcctt cctgacgatg atgaaatgtg
     4621 tcagtggttc tggacaagac tgaaacaaaa acaggatgaa acatctccgg tacataaatc
     4681 cgtgacccga tggttcatac cgtcctcaac gaaagaatat tatcttgcct ctctcgtcgc
     4741 ctttgcttta tggaaagagg cacctgatac cattgaactg ttcagaacac gaataaatca
     4801 tgcctggcat cagaaaaaac aaaaggacaa atatcggagt gatggtaaaa aggccattaa
     4861 tatacatata agaagcaatg caaaagaaat gcttgatgaa ctcagaactg tttacggtct
     4921 gagaacagga gagttttttg aaatgctgat tacagaaaaa tatattgaac acaaacgaag
     4981 gaagtaattc attacctgtt acattctaaa taatctcaga cagatattat ctccgtggat
     5041 cccccgcctc ctttccggtg cgcggggttt tgtctttttt caccgggaat acatgtatga
     5101 atccgtctga tgccattgag gcaattgaaa aaccgctttc ctccctgcct tactcacttt
     5161 cccgtcacat cctggaacat ctgcgtaaac tcacccgtca cgaacccgtg attggcatta
     5221 tgggtaaaag cggggccggt aaatcctcac tctgtaatgc actgtttcag ggggaggtca
     5281 ccccggtcag tgatgttcac gccggcaccc gggaagtgca gcgcttccgt ctgagtggcc
     5341 atggtcacag catggttatc actgacctgc ccggggtggg cgagagccgg gacagggatg
     5401 cagagtatga agccctgtac cgtgacattc tgcctgaact ggacctggta ctgtggctga
     5461 ttaaagccga tgaccgtgcc ctgtctgtgg atgagtattt ctggcgacag atcctgcagt
     5521 gcggacatca gcaggtgctg tttgtggtga cgcaggccga caaaacggag ccctgccatg
     5581 aatgggatat ggccggcatt cagccttctc ccgcacaggc acagaacatt cgggagaaaa
     5641 cagatgcggt attccggctg ttccggcccg tacatccggt tgtggccgta tcggcccgca
     5701 ccggctggga actggatacg ctggtcagtg cgctcatgac agcacttccc gaccatgccg
     5761 ccagtcccct gatgacccgc ctgcaggacg agctgcgcac ggagtctgtc cgctctcagg
     5821 cccgtgaaca gtttaccggt gcggtggacc ggatatttga cacagcggag agcgtctgtg
     5881 ttgcctctgt tgtccgtacg gccctgcgcg ctgttcgtga caccgtggtc tctgttgccc
     5941 gcgcggtatg gaactggatt ttcttctgaa cctgttgtgg atgatgccct ccctgcctct
     6001 gagcctgctc acaaaagcgc tgttttcgtt actgtctctc ttgtccgtgc aatagctgga
     6061 taatagaata aaacgatcga tatctatttt atcgatcgtt tatatcgatc gttatgctaa
     6121 taataactcc tgttagcaac gtgcgcagat agacacagac atgagattca gggaacaaca
     6181 gagccacacg tcagaaactg ccg
//
LOCUS       DAZGSP010000189         6188 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZGSP010000189 DAZGSP010000000
VERSION     DAZGSP010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.190"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1551)
                     /locus_tag="ACHK74_004943"
     CDS             complement(<1..1551)
                     /locus_tag="ACHK74_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020315086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4209 domain-containing protein"
                     /protein_id="HFP0223613.1"
                     /translation="MDDINISLDELNTIDLSPLIQSKLPVTCFDITKYLSSLGDGRAE
                     IRLLTHICGFHFRPENPQVPFGPCFQSSQGRSAIPDDINGLSLEVLSQFCPTIELPEL
                     QARIADTLWVRKIGGIRFPLLAVRAYYASSIAIMTSQGTWVSALERLERALRLCCFFR
                     KHTDFRDEFDQLSAHLLAEYERTREQGDSPYPLRLLQLDIDCKVSEPSFIAQELLFLT
                     KNYLAQKLFSFAVDACKTAIPIANRCCDRDTQFEFWRLLADTHLEESKFQDGGMISAA
                     CMQNAIEALANIPGTRIERLALYEEMRDYQIESRHQMSILQSPPQDISEIVHQAKSRV
                     VGRDLFDMVFRLAMLVCRPTSIERLKAQAIEQKANSIAWMFGSTHIDHEGMTLARIPA
                     GLGIDDANGAVIWPIMMTRMRIDHELAVAGQIIPATDEITMKYPISEAFFRDMFINHP
                     FIPFGHEEFFIKGMVCGFNGDFMTACHVLIPQLENSLRYVAKIKGEEPSQLHGDGSQE
                     RNGLKGLLD"
     gene            2226..2381
                     /gene="hokD"
                     /locus_tag="ACHK74_004944"
     CDS             2226..2381
                     /gene="hokD"
                     /locus_tag="ACHK74_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP0223614.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            2598..2849
                     /gene="rem"
                     /locus_tag="ACHK74_004945"
     CDS             2598..2849
                     /gene="rem"
                     /locus_tag="ACHK74_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFP0223615.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVNGRVTDGFILREGEFVTSLQALKEIGIKAGFLAFAEE"
     gene            2916..3194
                     /locus_tag="ACHK74_004946"
     CDS             2916..3194
                     /locus_tag="ACHK74_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223616.1"
                     /translation="MARNVKYYNSDNSPVLVCTHERYSHAFKSEWFQHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            3196..4245
                     /gene="ydfU"
                     /locus_tag="ACHK74_004947"
     CDS             3196..4245
                     /gene="ydfU"
                     /locus_tag="ACHK74_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0223617.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQSLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKTPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEEKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            4259..5011
                     /gene="ydfT"
                     /locus_tag="ACHK74_004948"
     CDS             4259..5011
                     /gene="ydfT"
                     /locus_tag="ACHK74_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP0223618.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDAQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(5186..5388)
                     /gene="cspF"
                     /locus_tag="ACHK74_004949"
                     /pseudo
     CDS             complement(5186..5388)
                     /gene="cspF"
                     /locus_tag="ACHK74_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
     gene            5687..5902
                     /gene="cspB"
                     /locus_tag="ACHK74_004950"
     CDS             5687..5902
                     /gene="cspB"
                     /locus_tag="ACHK74_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HFP0223619.1"
                     /translation="MSNKMTGLVKWFNAEKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAANVIITD"
BASE COUNT         1717 a         1329 c         1504 g         1638 t
ORIGIN      
        1 atctagtaat cccttgagtc catttcgctc ttgcgaaccg tccccgtgaa gctgcgatgg
       61 ttcctcacct ttaattttag ccacatatct cagactattt tcgagctggg gaatcaacac
      121 gtggcatgcc gtcataaaat ctccattgaa accgcaaacc atacccttga tgaaaaactc
      181 ttcatgccca aacgggatga agggatgatt gatgaacata tcgcgaaaaa acgcctccga
      241 gattggatat ttcattgtga tctcatctgt agcaggaata atttgcccag ctactgccaa
      301 ctcatggtcg atacgcattc tcgtcatcat tatgggccaa ataactgccc catttgcatc
      361 atcaatcccc aatcctgcgg gtatgcgagc aagtgtcatt ccctcatgat caatatgtgt
      421 tgatccaaac atccaagcta tgctgttggc cttttgttct attgcttgag ccttaagtct
      481 ctcaatgctg gttggccgac agaccagcat cgcaagtctg aaaaccatat caaacaaatc
      541 tcttccgact acccgactct tggcttgatg gacaatctca ctaatatctt gagggggaga
      601 ctgaaggata gacatctggt gacgggattc gatttggtaa tcacgcattt cttcatacag
      661 cgcaagacgt tctattcggg ttcctggaat attagccagt gcttcaatag cattctgcat
      721 gcaggctgcc gaaatcatac cgccatcttg gaattttgac tcttccagat gtgtatcggc
      781 aagaagccgc cagaattcaa actgtgtatc tctgtcacag cacctattcg caatcgggat
      841 tgcggtttta caggcatcga cggcaaatga aaataatttt tgagccagat agttttttgt
      901 cagaaacagg agttcctggg caataaaaga tggctcactc actttacagt cgatatcgag
      961 ttgcaggagt cgcagcggat aaggtgaatc gccttgttct ctggtacgtt catactctgc
     1021 tagtagatgt gccgaaagct gatcaaattc atccctgaaa tcagtatgct ttcgaaaaaa
     1081 acaacacagc ctaagcgcac gctctaggcg ttctaaagca ctaacccacg tcccttgcga
     1141 tgtcataata gctatactgg acgcataata agcacggact gccagcaaag gaaaacggat
     1201 tccaccaatt ttacgaaccc aaagtgtatc agcaatacga gcttgaagtt cgggtagctc
     1261 tatcgtcggg caaaattgag aaagtacctc cagcgacaac ccgttaatgt catccggaat
     1321 tgcagatcta ccttggctgc tttgaaagca cggaccaaat ggtacctgtg gattttcggg
     1381 acgaaagtga aaaccgcaaa tatgggttag cagtcttatc tccgctcttc catcacctag
     1441 gctagaaaga tactttgtga tatcaaagca ggtaactggc agcttactct ggattaaggg
     1501 ggataaatca atagtgttaa gttcgtcgag gctgatatta atatcgtcca tgtcgtcacc
     1561 ttgatgattt gttaatgcgg gggcaacatt aataaatgta cttgagaccg caatttgtcg
     1621 atggagccaa catttataac tatgtgcttc taccctctga atttttacat gtgaattgct
     1681 agcttgaata gctctatcat tatatacgtg gcactaaatt atggctgttt ttcgctcaaa
     1741 gcggactaga aggttagctt gtgtcggact ttgcgtatta aaagaagtgc tggtggtgac
     1801 tagtggttgt gccccatttc cacagaaaaa atcagagaaa ctatacccaa tagttgtatt
     1861 gaatcactga cgagacagcc tcatattcat caggactggt ctacgtccaa tacaggaggt
     1921 tgtggtgctg gttctcaaat gtgcgctggc tattacggct gtaatggcga tttattgtct
     1981 tgctattgtt cttatggatc gcctttctga ctgatttcac attggcgagg taacggtagt
     2041 taagtagaat ggctgcgggt gcttgaggct atctgcctcg ggcatgaaca ccaacggcag
     2101 atagagaaaa gccccagtta acattacgcg tcctgcaaga cgcttaacat taatctgagg
     2161 ccaatttcat gctagacata tgtaggttag cctcttacgt gccgaaaggc aaggagaagc
     2221 aggctatgaa gcagcaaaag gcgatgttaa tcgccctgat cgtcatctgt ttaaccgtca
     2281 tagtgacggc actggtaacg aggaaagacc tctgcgaggt acgaatccga accggccaga
     2341 cggaggtcgc tgtcttcaca gcttacgaac ctgaggagta agaaacccgg cgagggagaa
     2401 atccctcgcc acctctgatg tggcaggcat cctcaacgca cccgcactta acccgcttcg
     2461 gcgggttttt gtttttattt tcaacgcgtt tgaagttctg gacggtgccg gaatagaatc
     2521 aaaaatactt aagtagcgcg cagggataag agggatggtc ccttaaaggg gagagctaat
     2581 tatccggaag gattctgatg atgaacatcg aagaattgcg taaaattttt tgtgaagatg
     2641 gcctctatgc tgtgtgcgtt gaaaatggaa atcttgttag tcattaccgc attatgtgtt
     2701 tgcgaaagaa tggggctgcg ttaattaatt ttgtgaatgg tcgagtgaca gacggattta
     2761 tcttgcgcga aggtgagttt gtcacttcat tacaggcact gaaagagatc ggaataaaag
     2821 caggcttttt agcttttgca gaagaataaa ctcatctaca atcttgcgcg gggctgaact
     2881 cccgctgagt aacaccgtgc caccggagaa aaccgatggc acgcaacgta aaatattaca
     2941 attctgataa ttcgcccgtt cttgtctgca cgcacgagcg gtattctcac gcattcaagt
     3001 ctgaatggtt ccaacaccct ccatgcactg aagagcaggc tgaatggata attcagtgtt
     3061 accgcaggcg cggatacgag gttaagaaag cccttagcct cgactaccgt cactggataa
     3121 tctcagtcag attgccttac tccgaacgcc caccacgtgc gtcccgcact ttccagcaac
     3181 ggatctggag gtaacgtgcg ggtattactt agacctgttc tggtgcctga gcttgggctg
     3241 gtggtcctta agccgggccg tgaatccata cagatatttc ataatcctcg agtgctggtg
     3301 gaaccggaac caaaaagcat gcgtaatctg ccatccggag tcgttcctgc cgttcgccag
     3361 tcgctggcgg aagacaaaac attgctgccg ttttttagta acgaacgggt gattcgtgct
     3421 gctggcggtg ttggcgcatt gtctgactgg ctattacgtc atgttacatc ctgccagtgg
     3481 cctaatggcg attaccatca ctctgaaaca gtcattcacc gttatggtac cggcgcaatg
     3541 gtgttgtgtt ggcactgcga caaccaactg cgtgaccaga catcggaatc actggagctg
     3601 cttgctcaac aaaatctgac agcatgggtg attgacgtca tccgtcacgc aataagcggt
     3661 acgcaggagc gggaattatc tttggctgaa ttatcctggt gggcggtctg caatcaggtg
     3721 gtggatgcac tacctgaggc tgtatcgcgt cgttcgctgg gattaccagc ggaaaaaatc
     3781 tgctcggtgt accgcgaaag cgacatcgta ccgggagagc agaccgccac cagcatattg
     3841 aaacaacgca caaaaaatct tgcaccgttg ccttacgccc accagcaaca aaaaacacca
     3901 caggaaaaga cggtggtaag catcaccgtt gatccagagt ctccggaatc tttcatgaag
     3961 ctgcctaaac gtcgccgctg ggttaaggag aaatacacac gttgggttaa gacacagccg
     4021 tgtgcttgct gcggtatgcc agccgacgat ccgcatcatc tgattggtca cgggcagggc
     4081 ggaatgggaa caaaagcaca tgatctcttt gtgttgcctt tgtgcagaaa gcatcacaac
     4141 gagctgcata cggatacagt ggcatttgaa gagaagtatg gctcccaact ggagctgata
     4201 tttcgtttta tcgatcgcgc gctggcaatt ggcgtactgg cgtaagtgga gaacgagcat
     4261 gaaccttgaa gccttaccaa aatattactc cccaaaatct ccaaaattga gcgatgacgc
     4321 tccagcgaca ggcaccggtt gtttaacaat tacggatgta atggcagcgc aggggatggt
     4381 gcagtcgaaa gcaccacttg ggttggcctt atttctggca aaagttggtg ttcaggacgc
     4441 tcagtttgcg attgaaggcc tgctaaatta cgcgatggca ctggataacc cgacattgaa
     4501 caaattgagt gaagaaatcc ggttacagat tattccttac ctcgtgaatt ttgcctttgc
     4561 tgattactcc aggtctgcgg caagtaaggc tcgctgtgag cattgttcag gtacgggatt
     4621 ttataatgta ttgcgcgaag tggtgaaaca ctacagacgc ggggaatctg taatcaagga
     4681 agaatgggtg aaggaactat gtcagcattg ccatggtaag ggcgaagtca gcacagcgtg
     4741 cagagggtgt aagggtaaag ggattgttct ggatgaaaaa agaacccggt ttcatggcgt
     4801 accggtatat aagatttgtg ggcgttgtaa tggaaaccgg tttagtcgtt taccgaccac
     4861 gctggcacga cgtcatgtcc agaagctggt accagacctg accgattatc agtggtataa
     4921 ggggtatgcg gacgtcattg gtaaactggt aacaaagtgc tggcaggaag aagcatacgc
     4981 ggaagcgcaa ttgaggaagg tgacgagata aatgattttt gctgaagatg gcgacatgat
     5041 gtttgcattt ttcaaaaaac atggataaga ttttctcaac gatgggcttt gtgtatccga
     5101 cgtttagaaa aaagtagaaa acccgcttat aagcgggttt ttgtgcttta aatggggcaa
     5161 tagagatatt gaatctcatc ccgggataaa cattggcagt tgaaggtcca cgcgaaccat
     5221 ttatccagca aaattccacg cgtaatcctg tggtaatttc ttctgcatct cgaagattga
     5281 gagctgaaac gtgaagctgg gcatcgatac gccatcggat gggaatataa gacctttgct
     5341 gcttttgtag tcaaagtttt tgacaattcc tgtcatttta ggggacagaa aaactcctta
     5401 atactgataa cctggtgcac catacacacg ttcctggaga aaactacttt tttgatagga
     5461 ttgaaggtgg ctggatgtct aaaataaaca ttgcttcata tgttcaacta tgagttaatg
     5521 actgcgtcgg tttgaagaac agacgatata cgaagtagtt tactaaagca gttctcattt
     5581 caggtgttat tcacttattc cttctttgag tctctccaat taagtacgaa gtcgtttctg
     5641 ttatgcaaac catttatgcc gaaaggctca agttaaggaa tgtagaatgt caaataaaat
     5701 gactggttta gtaaaatggt ttaacgctga aaaaggtttc ggctttattt ctcctgttga
     5761 tggtagtaaa gatgtgtttg tgcatttttc tgcgattcag aatgataatt atcgaacctt
     5821 atttgaaggt caaaaggtta ccttctctat agagagtggt gctaaaggtc ctgcagcagc
     5881 aaatgtcatc attactgatt aaaattcatc gctcgtctgt atacgataac gaagaaggct
     5941 gatgcctgag tagagatacg gacagagtag tgaatattgg atctctttaa taaaaagtaa
     6001 ggaggtccaa tacatgaaac aatggctagc atatttggca aaatcttaat caggaaaagt
     6061 atgctaacca ttgtggtgaa gtgcaggttt gctgcatgaa tagtttcaca gcagaagcta
     6121 actgctggca tcgcaaaaca aagtgcgtaa gtggatgact cccacaaaaa gcaccacaat
     6181 ctcaaacc
//
LOCUS       DAZGSP010000190         5986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZGSP010000190 DAZGSP010000000
VERSION     DAZGSP010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.192"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(112..2832)
                     /locus_tag="ACHK74_004951"
     CDS             complement(112..2832)
                     /locus_tag="ACHK74_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFP0223620.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     HSIGSQPVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYGLIQCRSLIEEMIADGDF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAAADIFERVKPECRYNPELAASDEVEPGFRRDPEIRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRT
                     TNADRCVVLCEPGGTPLFNNVAKLEARLQKNGSVHFRDTRTGKNVCTDYGDRVVFYHH
                     TDRNELAEKLNLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFT
                     ISRPDVDLHRQRSEEYYHEYIRQQTRLSESHDENYSSRQENTWVPPSPGM"
     gene            complement(2844..3176)
                     /gene="mobA"
                     /locus_tag="ACHK74_004952"
     CDS             complement(2844..3176)
                     /gene="mobA"
                     /locus_tag="ACHK74_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFP0223621.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            3409..3744
                     /locus_tag="ACHK74_004953"
     CDS             3409..3744
                     /locus_tag="ACHK74_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFP0223622.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKTSRRSSSKTAPKKTDSAR
                     KDGIDMNNN"
     gene            complement(3829..4677)
                     /locus_tag="ACHK74_004954"
     CDS             complement(3829..4677)
                     /locus_tag="ACHK74_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP0223623.1"
                     /translation="MNQTLPTADLNTDGTTDVIPSVAIDRIIAQRNEGISLFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVWRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRS
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            5026..5222
                     /locus_tag="ACHK74_004955"
                     /pseudo
     CDS             5026..5222
                     /locus_tag="ACHK74_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5256..5507
                     /locus_tag="ACHK74_004956"
     CDS             5256..5507
                     /locus_tag="ACHK74_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP0223624.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(5538..5807)
                     /locus_tag="ACHK74_004957"
     CDS             complement(5538..5807)
                     /locus_tag="ACHK74_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223625.1"
                     /translation="MRASQPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKEGFKQG
                     FKQGALEVAREIACRLRDIGWPPERIQEVTGLSGEELKKLFPDEQ"
     gene            complement(5807..>5986)
                     /locus_tag="ACHK74_004958"
     CDS             complement(5807..>5986)
                     /locus_tag="ACHK74_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0223626.1"
                     /translation="ERLPQHEDKLMTIAERLEQKGALEKALAIARQLQKMGMTPEQIK
                     QATGLSEAELKKVIH"
BASE COUNT         1459 a         1645 c         1359 g         1523 t
ORIGIN      
        1 ggcagcataa aaatttccct caataaaaat caagcttttt caacgggcta atctgacata
       61 tagctctgat gaattcaatt cactggattt tgcaccatca tgtatccgga cttacattcc
      121 aggtgacggc ggcacccacg tattctcctg ccgtgagctg taattctcat catggctctc
      181 cgataaacgt gtctgctgcc tgatatattc gtggtaatac tcttcactgc gctggcggtg
      241 taaatccacg tccggacgac ttatggtgaa gtgtccattt ccggttattc ctgctgcatt
      301 gtgccaggcc accatttctg caaacacatc attaaactgc tgatatgaac cttccggttc
      361 aaaaccaagc tttccgttgc ggctgaacag aactggcgca attaaattaa gcttctctgc
      421 cagttcattt cggtccgtgt gatggtagaa cacaacccga tctccgtaat ccgtacagac
      481 attttttcct gtccgcgtat ccctgaaatg aacacttcca tttttctgaa gacgagcttc
      541 aagttttgca acattattaa aaagtggcgt tcctccgggt tcgcagagta cgacacaacg
      601 atcagcatta gttgtgcgtc ttttatcctt attacgcgag cgacgatcac gataatccca
      661 gccacgtaac tgcgatactg cagccctgtc tccctgagcc gcctgttgtt ccacccactg
      721 ccggtaggac aacggatacc acttaccctc cgcaatcagt gacaaccgct cttccttcac
      781 actctccttc agcctgatca gcgcctgcat acgctgcact tccgcaatat ggtaatgcag
      841 cttacgcaat tgtggatccc ggaactgcac acggatatac gccttgcgcc gacggcaggc
      901 agcatgaatt tcccgtaacc gttctccgta acgtaagtcg ggtttacgcc agtgctcctt
      961 ccatgcaaga taacgcgcac gcaaatcttc ccgcgcagct gcacgggctt cacggcgctc
     1021 acgacgaatc tcaggatcgc gccggaagcc gggttccact tcatcactgg cagcaagttc
     1081 cggattgtaa cgacattcag gcttcacccg ttcgaaaata tctgcagccg caatttcaaa
     1141 tggcccggcc tgaggctcgg cacgggataa cgtcaaatca ggatggatac tgctggcctt
     1201 caccggtgtc agctcatgat taaacgcatc cacaatcaca agaccatgat gctgtttctg
     1261 cagcataagc cctttacgcg caaacatctc atgacatgcc tgccagctga aatccccgtc
     1321 agctatcatc tcttcaatca ggctcctgca ctgaataagg ccgtatcccc acagactttc
     1381 cttatcagaa aacagttcac gctttatagc cccctgcgga cctgtgccca tccgcatacg
     1441 ataccattcg taatcactga caggctgaga gccaatactg tgtccccgga atcgttctcc
     1501 gggtataacc tgagtaaaaa tatccttcgg tacaggctgc catccaccat ccagatcctg
     1561 catataatcc agcgtcatta acggccacac actgtctgca cgtactggtg tctgttgttt
     1621 atcaaatcca tcatgcagta ccagatgccc gtgctggatc cccatccata atccagcctt
     1681 tgccagagtt ctgtgcacat ccaggcaact ttccagctca ccatttctcg ccatttccgg
     1741 taagcgttcg gcaaaatgac ggcaggcgta ctgtttcaga ctctccgttt ctgccacttt
     1801 ttccggccgg atatcaacgt ttatggcttc cggatcataa cgaccgggtg tccccacctg
     1861 actgaaaata tctgtcggta caggctgata ttcccccagt ttccgcccca gtttttcagc
     1921 tgtccatgac ggtccgaacg aactgagtgc aacaccttcc cgcgctctgt cccagccatc
     1981 cttcaccacc agttcccctt cctgcatcgt gatatacaga ccatcactgg ccagccgttt
     2041 atgcaaagat aaccagtcct gtgcaggctc ttcacgcagc ccggcaatcg acatctgagc
     2101 aatatattcc ctgaacgtct gctttttccc gcgtcgccag gcattacggc gctcacgtac
     2161 cagcgctgtt ttgcgcacaa tacggttgcc gggtgcatgc acaaagcagc cattatccgg
     2221 cgcaaatccg tgcttaagtt caagttcacg acatgcacgg ctgagttttt cctggctcca
     2281 ggaaagccag ttaatataac cagtttcagg atgtacacga ttcacggcaa catgcacatg
     2341 cagattatcc gtgtccgtat gaacggcagc cacatactga tgttttgaga gttcaagaga
     2401 tttcagtgtg tgacgcacac agtcaaaaag ctgctcagga cgcgggcttt catgcgcagg
     2461 ccaggaaaga atgtagtgga aaacggggtc tgtatcatta cgggaaaaaa cagctttgtc
     2521 cgccttgtac tgcatctctt cagcagctgt ttccagtgaa ttgcagttat ggaaacaggt
     2581 tacgccatag aaatttaccc actgactgcc gtctttcatc acatcaatca gggagacaaa
     2641 tttttcgtta cgcaggcgtg ttgcataatc aacaagccgt ctgaaacggt tgcggtgagg
     2701 gacatcactc ttgatttcgc tgtcttctgt gagctcattt tcctgcacat catcccgcac
     2761 agacgtgtac gcaatcaggt cttcaaagga agacctgccg tcacgtcttt tgtgcggaat
     2821 aatggcattc atcagcagtt atctcagcgt cccattaagt ctcttcgcag agcattgaca
     2881 tattcagtaa tggcaacaag aacctctgca tactccttat caccagtgcg ttttccctcc
     2941 acaaacagat gcttctgaag gcggccaaga cgcataatct catcaatcat acggtcgtca
     3001 gtacggcagt taaccacctt atccagtgaa gagcttcgta taaatgttga aaccgtctgt
     3061 ccacttgact ccgctttttc tcttacgatt tcagcctcat cctccgtgaa tctcgccgtg
     3121 aattttacaa ttctcttcct gttctcactg cccgttcggg ttttcttctc actcatggtc
     3181 tgctccttac cggggtgtcg gggcaaagcc ctgaccaggt ggggaatgtc tgagtgcgcg
     3241 tgcgcggtcc gacattccca catcctgtcc cgtttttcag gccattatag cctgttattt
     3301 ttgtgcatca tagtcttatg cagacggcag aggcagggcg cagccctgcc aggaggcagt
     3361 ttttgatgat aaaatcagac ttcaacatga aacggagaga aattgccaat ggcttctaaa
     3421 aaattttact cagatgatga catccagctg gcaaaagctg ctttatcaga attgccggac
     3481 ctcaccgctc aacggaaaac attgcgtgat tttcttgatg ccattcgtga tgacatcatt
     3541 gtcctggtaa ggacaaaggg ttatacactg gcagatgttc gtgacacgct caaaaatgca
     3601 gggtatgaag taggagagaa ggcacttcgt gacattattc gggaagcaga atcgaaaaaa
     3661 acatcccgcc gttcttcctc taaaacagca cctaaaaaga cagattcagc caggaaagat
     3721 ggcatcgata tgaacaacaa ttgatgtgac aatgtcacat cgtattctga gattatgatg
     3781 attacagaga aaaagccctg gcataaatac cagggctttt ctgtttaatc agactcgtgg
     3841 cggcaatgca ccaggataat gccgtgcaat aatatcattc agtttttcaa caaggtcagg
     3901 acgcttaaag acaatgtgta cggttccttt ctggtagcca cgcaccgtaa aatattcccc
     3961 ttctaacagc ttaccgacat tgtctccgtt cccgagaaat tcaccatact gcgcaccgat
     4021 gctgctccgg aagtcaggaa cgttgcgccc ctccagcaga taaaacggac gtaccaggtc
     4081 atccagtgcc tcccgtccgc tgcagtccag cgtcacacgc ccgttactcc agcggtacag
     4141 caggttttca atgatgattt tcttgcccag acggcaggga ttattggttc tgtaatccca
     4201 tgacaatttc cgccagacat caatcagtcc ctgttcaaat gtctgcatct tactggcatt
     4261 cagatggcga aaagttgcca gtacattgtc cagggttatt tcaggacagg tgtcgctcat
     4321 cagctggctg ttccactcat cacgctgaca tgaactcatg aaggtgtaca tccccgtatc
     4381 cgtcatcagg cggtcccaga ttttccggtc ggcaagacgg gtgatattcc ttttcgcttc
     4441 ttccggttta tccatgcatc gaacggagtc agtcacgcag tcttcaaacc cgtagagaaa
     4501 aatgtcaccg gaagcatcga gcagaatcct gcgtgctgtc gccaggcatt ccatcgcctg
     4561 catgaacagt gaaatacctt cgttacgctg cgcgatgatg cggtcgatag ccacagacgg
     4621 aataacatct gtcgtgccgt cagtattcag gtcagcagtg ggtaaagtct gattcatcac
     4681 attgtctcct catcgtcacg actgccgggt cagcctggtc atttcccgga ggagcgaagc
     4741 tccttctgcc gtcctggtct tcgtggattt ccccgcagtg gacctttccg acgggggacg
     4801 ctcaccacgt cacggggcag ttgtccgggg gcagacgaaa aagtttttga cgcctgcggc
     4861 aaaaagtttt gtggctggta catctctccc ctggacgact gaaacgggat gtggtcgtgt
     4921 atgtaccccc ggaaagtccg tctgcggaga acaggccgca gtgcggcggc agacaggcag
     4981 agacagctgc gccatcaggc gcagtgacct tacacaccgt caggtgtgca gggagcgccg
     5041 catccggcgc gactgaagaa aaacgatggc acaacaatgg catgtggtgg tcactgacgc
     5101 accactcccg cacaggtgaa aacagagaaa accgcacgac atctacaata aaaaaagtga
     5161 aatttcttca ctttttcaca cttatttatc gcgctgattt ggtgattatg atgataaaat
     5221 aaagtgaagt tttttcattt tttcaaaggt gttttatgac cggctgggaa ctgcgaatct
     5281 ggcgaaaaag catgctctgg tcacgcgaaa aagcggccag agaatttggc gtgacgcaga
     5341 gaacatggca cgcctgggaa aatgcggaac aggtggacgt caccgtctgg aggaccaccc
     5401 aggcactttc agtccgtgac ctgctgcctc acatgcaggg gatgcgcaag gcagatatca
     5461 tcaggcgtct ggaaaatgaa ctgggggaaa cggcagggaa tgtgtgagcg tgattcacag
     5521 cctggctgaa tgccaggcta ctgctcatca ggaaacagct ttttcagttc ttcaccggaa
     5581 agtcccgtga cctcctgaat ccgttccggc ggccagccta tatcccgaag ccggcaggct
     5641 atttcccgcg ccacttcaag agctccttgt ttaaaacctt gtttaaaacc ttctttaaaa
     5701 ccctcgtcga aacctttctg tataagacgt tctgcaatcg tcatgctgtt tgctctgctc
     5761 ctgctgacaa aaaaacccgg cattttgccg ggctgtgatg ctctcatcag tgaatgactt
     5821 ttttcagttc agcttcggaa agtccggtgg cctgcttaat ctgctcaggt gtcatcccca
     5881 ttttctgaag ctgacgcgca atggccaggg ctttttcaag cgcacctttc tgctcaagac
     5941 gttctgcgat tgtcatcagt ttatcctcat gctgcggcag gcgctc
//
LOCUS       DAZGSP010000191         5880 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZGSP010000191 DAZGSP010000000
VERSION     DAZGSP010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.193"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..252
                     /locus_tag="ACHK74_004959"
     CDS             <1..252
                     /locus_tag="ACHK74_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0223627.1"
                     /translation="ERLPQHEDKLMTIAERLEQQGIQKGIQQGIQQGIQQGIQQGIQK
                     GRMEEKQDIARQLQKTGMPSAQICQITGLSEAELKNITH"
     gene            complement(554..1741)
                     /locus_tag="ACHK74_004960"
                     /pseudo
     CDS             complement(554..1741)
                     /locus_tag="ACHK74_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(1741..2106)
                     /locus_tag="ACHK74_004961"
     CDS             complement(1741..2106)
                     /locus_tag="ACHK74_004961"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223628.1"
                     /translation="MASSAKPRKRKPAPQRSKLPRYVVKLHDDDFFDEEDAEALRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVPPPF"
     gene            2197..2730
                     /locus_tag="ACHK74_004962"
                     /pseudo
     CDS             2197..2730
                     /locus_tag="ACHK74_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3143..4645
                     /locus_tag="ACHK74_004963"
     CDS             3143..4645
                     /locus_tag="ACHK74_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223629.1"
                     /translation="MKKFYQFRDEQRKELEQHDFYSLISSDCIALKDKLLFAPVMAHF
                     IMNFRDMNKWVIRFDNNDNEYKSVINGGTIEDETHSRLFLEDWRKLYIDDKLNWKASD
                     VIYWLFISREMECFRKFGIDFMRLCVDDGGEPILRYSHSESGETCGNIFFSKISPIAD
                     QVANHLGISLRYFGTFHLNLENGHVWKSEGVFENIELSPDSYKKMATLSKRMFDIFEG
                     IHDSFYNYLSSYVLNGSHPSFFESLPVGKNVAPIYPEFVIENKSHNDGRHIEHINNYL
                     EKISSHEFFKWLINTSIDPQLKLKSFIPLWIVDIMGYRDINKYVFTYEQPESESEKII
                     NDYALHLSEHSRLFYHDWKSLQLDDMLRWSASDTLEFIFLNADMDMHRENIVKFSLFG
                     LKHRDPVIRFWFMMILELSGKEFFSHVGDIALQVESKYNIYLPYLCGRHATENEHEAY
                     NNMYEHFMVKEISHEQSDLIIQITDMVMRSLLNNLDISYRYVVNNLLAAR"
     gene            5431..5526
                     /gene="dqlB"
                     /locus_tag="ACHK74_004964"
     CDS             5431..5526
                     /gene="dqlB"
                     /locus_tag="ACHK74_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFP0223630.1"
                     /translation="MINLLIIVLRAVVAVANALIAILELIRQFID"
BASE COUNT         1680 a         1295 c         1335 g         1570 t
ORIGIN      
        1 gagcgtctgc cgcagcatga ggataaactg atgactattg ccgaacgtct tgagcagcaa
       61 ggaatacaga aaggaataca gcagggaata cagcagggaa tacagcaggg aatacagcag
      121 ggaatacaga aaggccgcat ggaggagaag caggatattg cgcgtcagct tcagaaaacg
      181 ggaatgccat ctgcacaaat ctgtcagatc accggacttt ccgaagctga actgaagaac
      241 atcactcact gatgagaacc acaaagcccg gcaaaatgcc gggttttttt gtcagcagga
      301 gcagagcaaa cagtatgacg attgcagaac gtcttataca gaaaggtttt gaaaaaggtg
      361 ctcttgatcg ataggaattt aaaacccaaa aagattaaaa aaacacccaa aaacggatgt
      421 ttattcaaca ctgcctgcgt gccatgagtg caccgccgtc aattaaatgg atcttatcga
      481 ttgatactgc atatcactga tggtttttta ctccattccc ctgaaaatgg acgcaacttc
      541 cccgatgcgg ctctcagccg cacacccgct gttgcgccag tggctcatgc atcaggacca
      601 gctctgtcag acggtagccc cgcttcagcc ccgtaaaacg catctgacat ccgcacagga
      661 tgcacttcag cgggtcaacc ttcagtaacc gctgatacat ccctctccac ctgatttgca
      721 tcgccgtctt tctcaccgtc tccgttatga cctacaccac ttcttccagt aaccggcgct
      781 tcaccggact caggaaaccg tagtacctca ccatacggaa ccccttatcc gccacatgcc
      841 aggagaacct ttccataaac tcatcaccac tcatcaccag gtattcttcc cgttttgtcc
      901 ggtgactgtt gtaacgcaga ccaatttcat cctgaccagc atagtgctcc agacggctca
      961 tcggcaccgg cggttttttc aggtaagagc cgaagtacac cgccacatgt gtggcgttat
     1021 ccatcacccg ggatacgttg acattccagc cccgccggta atgactgtcc aggaagcgat
     1081 tccagtcccg tctgctgctt ccttcaactg ccagcgcatc cggtatcacc agctccgggt
     1141 atttccgtga cagcaaccgt gttatccggt aacgccacat gctcatcacc ttacgggcgt
     1201 aaaaatgaag gtttttccag gtgtggtctg gtgtcacgcc gccggccgtt gtcgacaggt
     1261 gaatgtgcgg atgccactgc tggtcacgtc cccatgtgtg gatcaccgtg aatatccccg
     1321 gcaccacatc tgcctggagg cagatttcct gtatcacatc cgcagcaatg cggctcatct
     1381 ctgccagtaa ccaccggttg tggaacacca gggaccagta ctggcaggga agtgtgaaca
     1441 caatatgctg ccacggacag tcgggaacca gactcagcag atactgtatc cactgtgcgc
     1501 cagccttcac tccgcagtgc gggcaggagc gacttttaca ccggaagcag accttttttg
     1561 tgtggcaaca gtccggagat gaacagcacc actgtgtgta ccccatcagc gtggttccgc
     1621 acgccatgat ttttgtcacc gactcaatga ccaccggacg cactgaacct tccggttgtt
     1681 tctccagcca gttaagccag cggtttccct gctgaaaaat gtcggcaaaa cggggaagca
     1741 tcagaagggc ggggggactc cgtccggcca gtgaactgtg ccacactccg ggcaatacat
     1801 gccgccggcg ctgataccgg caagaatggt cgcaaactcc cgctccgtgc agcgggctat
     1861 ttcaggatac ccttcgtcat caacacgaac aaaccagaag accagctttt tgtttccggc
     1921 atccacaaag aagggaatat tcaggtctgc gcagcactca acggcatcgt caaaattatc
     1981 aaagcgcaga gcttctgcgt cttcttcgtc aaagaagtca tcgtcgtgaa gcttcacgac
     2041 atagcgggga agtttgcttc tttgaggggc aggttttcgt ttacggggtt tagctgaact
     2101 ggccatataa ctaactgaaa gacaatgata ttgtctgttt ttataacggt aatttcaggc
     2161 catgacaagc cgcagccgcc aggctgccta ctcgggcggc agttcgcagt gacagagcca
     2221 aatcaggtat ggtgcggcga cgtgacgtac atctggacgg ggaaacgttg ggcatacctt
     2281 gccgttgttc tcgacctgtt tgcaaggaaa ccggtaggtt gggcaatgtc gttctctccg
     2341 gacagcagac tgaccatcaa agcgctgaaa atggcctggg aaatccgcag taaaccagcc
     2401 ggggtaatgt tccacagcga tcagggcagc cactatacaa gcaggcagtt ccggcagtta
     2461 ctgtggcgtt accagatcaa acagagtctg agtcgacgag gaaattgctg ggataacagc
     2521 ccgatggagc gcttcttcag gagtctgaaa aacgagtgga taccggtgac gggttacatg
     2581 aacttcagcg atgctgccca tgaaataacg gactatatcg ttgggtatta caacgcgctc
     2641 aggccgcacg aatataacgg tgggttgcca ccaaatgaat cggaaaaccg atactggaaa
     2701 aactctaaag cggtggccag tttttgttga ccactacagg ggaagatagt gcaactcacc
     2761 gatgatgaaa aaagtggacg aagagacgat aactacactc ccatctaggg atgctttttt
     2821 tgtggtactg tagtgtggga gtaagttcgg gtttcatact gaatgaggcg attgcgagct
     2881 gcctttcatc gaaaactaat gtccattgtc attgtattgt ctgactctat ctcgtaacaa
     2941 gtctttgaca caaagtttta tatttttatc aaagtggaca tgaggtgaat taaatttgtg
     3001 atctttttca aataatgtgc tcttgatagt agattctggt tagtgatatc tattaacaat
     3061 atattaaatt tcttttgtat tatcgctgat aaaactactg tattggtatt ctatatatta
     3121 ttgagtaaca ggagatatca ctatgaagaa attttatcaa tttagggatg aacagcgaaa
     3181 ggagcttgaa caacatgatt tttatagttt gatttcttca gattgtatag cgttgaaaga
     3241 caaactatta tttgctcctg ttatggctca tttcataatg aacttcagag acatgaataa
     3301 atgggttatc agatttgata acaatgataa tgaatataaa tctgttataa acggtggaac
     3361 aatcgaggat gaaacacatt caagattgtt tctggaagac tggaggaagc tatatataga
     3421 tgacaaactt aactggaaag caagtgatgt tatatactgg ttgtttatta gtcgagaaat
     3481 ggagtgtttc cgaaaatttg gtattgattt tatgaggctt tgtgtagatg atggaggaga
     3541 accaatactt cgatattctc actccgagtc aggagaaact tgcggtaata tattcttttc
     3601 aaaaattagt cctattgctg atcaagttgc caatcatttg ggaatatcac tccgttattt
     3661 tggaacattt caccttaatc ttgaaaatgg acatgtatgg aagtcagaag gtgtttttga
     3721 aaatatagag ttgtcaccag attcttataa gaaaatggct actctctcaa agagaatgtt
     3781 tgacatattt gaagggattc atgactcttt ttataattac ctgtccagtt atgttcttaa
     3841 tggaagtcat ccgtcatttt ttgaatcatt acctgtaggg aaaaatgttg cacctatata
     3901 ccctgagttt gtgatagaaa acaaaagcca taacgatggt agacatattg aacatataaa
     3961 caattacctg gaaaaaatat cgagtcatga gttctttaaa tggctgatta acacctcaat
     4021 agaccctcaa ttgaaattga aaagtttcat acctctttgg attgttgata ttatggggta
     4081 tagagatatt aataaatatg tttttacata tgaacagcct gaatcagaaa gtgaaaagat
     4141 tataaatgat tatgcattac acctgtcaga gcatagccgt ttattttatc atgactggaa
     4201 gtcacttcaa cttgatgata tgttacgttg gagtgccagt gatactcttg agtttatttt
     4261 tcttaatgca gatatggata tgcatagaga aaatatagtt aagttttctt tgttcggatt
     4321 aaaacacaga gatcctgtta tcaggttctg gtttatgatg atactggagt taagtggaaa
     4381 agaatttttc tctcatgttg gagatatagc tttacaggtg gaaagtaaat ataatattta
     4441 tctcccatat ttatgtggac gccatgcaac agaaaatgag catgaagcat acaataatat
     4501 gtatgagcat tttatggtaa aggaaattag ccatgaacaa agtgatctaa taatacaaat
     4561 tacagacatg gttatgcggt cattattgaa taatttggat atctcatatc gatatgtagt
     4621 gaataattta ttggctgctc gttagaagaa cagcattggt atgactctga tgctccttaa
     4681 aatcccactt ttcagagtgg gatttgttta atgcccagaa cagggggaat ttccattcat
     4741 gcccaaaaaa tattttgatg ccctcccgcc aattctcgcg ttcagtcctt cctgaaaaac
     4801 ctcagaattc acgtagcgca tcgccgctac tgcatccatt aaaaaactga cagttttttt
     4861 aatcatctcg tggactcaac ttactgaaaa aatgggattt atgaaaatgc caccgtcggc
     4921 atcaagcagt tggtcagcgc aaccggctgc atcgactgca acaaaaaaac tgaaagagaa
     4981 agcccctcat atgagggaga aaaaaaggaa gcgcagtcaa cgagaggcgt aaaaaaaccc
     5041 gactggtgag gtcgggtttt aatacggttc cggttctgag ttggtggctc tgaccggagg
     5101 aagtcactct acacctaacg tttacatttt atgcactcag ccgcagtatt gcaactaact
     5161 gtggttaagg caccacattt agttgtgtat cgtgggtttt gtatacctta taatcatgaa
     5221 gttagccgtg tggtgtggct tcttcctctg cccttcccgt ttatgcaggt atgaacgatt
     5281 ttaaagtcct ggctgaacaa acaggtggtg ctgtgtttag tgctctggca ggaggaagtc
     5341 actctgcacc taacaatgcg agagaaccgt tgtgacaggc agtgtaagag caagcaacgg
     5401 tgatttcctc cagttttact ggagaaacgg atgattaatc tgttaatcat cgttctcagg
     5461 gctgtggtcg ccgttgcaaa cgcgctgatt gcaattctgg aactgattcg ccagttcatc
     5521 gactgatgac agcggaaacg taactaaggg caggaagtga accgcttcct gccctttaac
     5581 gtactgtgac atttttcaaa aaaactcacc aatattctcc tcggcagaaa tgacataact
     5641 cactgtaaaa taaaaaatca tctaaacgct ggctttgcat cgagcgcgtt ggccaacgct
     5701 tttatcagct gcgaccgcac cagataattt gaacaagatt tcccgcatta cctggacata
     5761 caggttattt tgtaggctgc gctcctccaa gccagttacc tcggttcaac gagcgccaac
     5821 tccataaacc tttgaaaaac cgcccaccaa ggcggttttt tcgttcagcc agacgacaca
//
LOCUS       DAZGSP010000192         5701 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZGSP010000192 DAZGSP010000000
VERSION     DAZGSP010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.194"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(309..557)
                     /locus_tag="ACHK74_004965"
     CDS             complement(309..557)
                     /locus_tag="ACHK74_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP0223631.1"
                     /translation="MRIEICIAKEKICKMPKGSVPALQQEMLRRVGKRYDDVDVIVKS
                     ASNDGLTILRAVDKESAQEFVQETLQEVWETADDWFIR"
     gene            776..2362
                     /gene="prfC"
                     /locus_tag="ACHK74_004966"
     CDS             776..2362
                     /gene="prfC"
                     /locus_tag="ACHK74_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFP0223632.1"
                     /translation="MTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGT
                     VKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTL
                     TAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENE
                     LKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAV
                     GEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAP
                     APMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVR
                     TAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNF
                     APELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVS
                     RLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSMV
                     NLRLAQERYPDVQFHQTREH"
     gene            2755..3360
                     /gene="osmY"
                     /locus_tag="ACHK74_004967"
     CDS             2755..3360
                     /gene="osmY"
                     /locus_tag="ACHK74_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFP0223633.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            3487..3648
                     /locus_tag="ACHK74_004968"
     CDS             3487..3648
                     /locus_tag="ACHK74_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFP0223634.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            3770..4843
                     /locus_tag="ACHK74_004969"
     CDS             3770..4843
                     /locus_tag="ACHK74_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFP0223635.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            4840..5622
                     /locus_tag="ACHK74_004970"
     CDS             4840..5622
                     /locus_tag="ACHK74_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0223636.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEACLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVLDVLCELRPEPEDEIAEVLLNNTYAVFSVSG"
BASE COUNT         1412 a         1388 c         1516 g         1385 t
ORIGIN      
        1 ataaacacga agacggtgaa cacctttctg tatatgggac ttggtaaaaa cggtcttaag
       61 tacaggagtg cgaagcacct ggttggacgt gcgtagccgg aatgcatatt cgtgaagtcg
      121 ggtaatgaga gttgaacggt ggcagtgaga atggagcagg aaaaaatcag atgcggaaag
      181 cgaaaataca cagcgggtgc gatgtgcttc atgctgtttg tatggctggg tggtgtggta
      241 acactctgtg tacttgctgc actgataaca caataaaaga ggggagctgc cagctccccg
      301 tgctgatgtc accggataaa ccagtcatcc gctgtttccc agacttcctg cagcgtttcc
      361 tgtacaaatt cttgcgcgga ttctttatcc acggctctta aaattgtgag tccatcatta
      421 ctggcagatt tcacaatcac atctacatca tcataacgct taccgacacg tcgtagcatt
      481 tcctgttgca gggcaggaac agagcctttt ggcattttgc agattttttc tttagcgata
      541 cagatctcaa tacgcataac acctcctgtc actgtgtttg tatacagtgt tatttttaac
      601 tgtatgaata aacagtgtca atagagcaaa gattgctgca tatacacatg ttctttggct
      661 acaaatacac ctactgatca tgttctgcta tgctctgata cctccaaaat gtacaatcag
      721 cggcatgaat ttttctttgt acatatgatt gtacataatc aaagagacaa caattatgac
      781 aagccttttg gccgcagagg tagcgaaaag aagaaccttt gcaattattt ctcacccgga
      841 cgccggtaaa actaccatca ccgagaaggt gctgctgttc ggacaggcca ttcagaccgc
      901 tggtacagta aaaggccgtg gttccaacca gcacgctaag tcggactgga tggagatgga
      961 aaagcagcgt gggatctcca ttactacgtc tgtgatgcag tttccgtatc acgattgcct
     1021 ggttaacctg ctcgacaccc cggggcacga agacttctcg gaagatacct atcgtaccct
     1081 gacagcggtg gactgctgcc tgatggttat cgacgctgca aaaggtgttg aagatcgtac
     1141 ccgtaagctg atggaagtta cccgtctgcg cgacacgccg atcctcacct ttatgaacaa
     1201 acttgaccgt gatatccgcg acccgatgga actgctcgat gaagttgaga acgagctgaa
     1261 aatcggctgc gcaccgatca cctggccgat tggctgcggc aagctgttta aaggcgttta
     1321 ccacctttat aaagacgaaa cctatcttta tcagagcggt aaaggccaca cgattcagga
     1381 agtccgtatt gttaaagggc tgaataaccc ggatctcgac gctgcggttg gtgaagatct
     1441 ggcacagcag ctacgtgacg aactggaact ggtgaaaggc gcgtctaacg agttcgacaa
     1501 agaattgttc cttgcgggcg aaatcactcc ggtattcttc ggtactgcgc tgggtaactt
     1561 cggcgttgat catatgctgg atggcctggt ggagtgggct cctgcgccga tgccgcgtca
     1621 gactgatacc cgtaccgtag aggcgagcga agacaaattt accggcttcg tatttaaaat
     1681 tcaggccaac atggacccga aacaccgcga ccgcgtggcg tttatgcgcg tagtatccgg
     1741 taaatatgaa aaaggcatga agctgcgcca ggtgcgtact gcgaaagatg tggttatctc
     1801 cgacgcgctg acctttatgg cgggtgaccg ttcgcacgtt gaagaagcgt atccgggcga
     1861 tatcctcggc ctgcacaacc acggcactat tcagatcggc gacaccttta cccagggtga
     1921 gatgatgaag ttcaccggta ttccgaactt cgcaccagaa ctgttccgtc gtatccgcct
     1981 gaaagatccg ctgaagcaaa aacagctgct caaagggctg gtacagcttt ccgaagaggg
     2041 cgcggtgcag gtgttccgtc cgatctccaa caacgatttg atcgttggtg ctgttggtgt
     2101 gctgcagttt gatgtggtgg tatcgcgcct gaagagcgaa tacaacgttg aagcagtata
     2161 tgaatcagtc aacgttgcca ctgcccgctg ggtagaatgt gcggacgcga agaaattcga
     2221 agagttcaag cgtaagaacg aaagccaact ggcgcttgat ggcggcgata acctcgctta
     2281 catcgctacc agcatggtca acctgcgcct ggcgcaggaa cgttatcccg acgttcagtt
     2341 ccaccagacc cgcgagcatt aattcttgcc tccagggcgc ggtagccgct gcgccctgtc
     2401 aatttccctt ccttattagc cgcttacgga atgttcttaa aacattcact tttgcttatg
     2461 ttttcgctga tatcccgagc ggtttcaaaa ttgtgatcta tatttaacaa agtaatgaca
     2521 tttctgacgg cgttaaatac cgttcaatgc gtagatatca gtatctaaag ccgtcgattg
     2581 tcattctacc gatattaata actgattcag aggctgtaat ggtcgttatt catcacccat
     2641 cgcttttgtg atggcgacca ttgacttctg tagagggtga agtctctccc tattcagcaa
     2701 tgcaacctcg tgttgccagg ctcaaattac gagcaaacat acaggaataa atcgatgact
     2761 atgacaagac tgaagatttc gaaaactctg ctggctgtaa tgttgacctc tgccgtcgcg
     2821 accggctctg cctacgcgga aaacaacgca cagactacca atgaaagcgc agggcaaaaa
     2881 gtcgatagct ctatgaataa agtcggtaat ttcatggatg acagcgccat caccgcgaaa
     2941 gtgaaggcgg cgctggtgga tcatgacaac atcaagagca ccgatatctc tgtcaaaacc
     3001 gatcaaaaag tcgtgaccct gagcggtttc gttgaaagcc aggctcaggc cgaagaggca
     3061 gtgaaagtgg cgaaaggcgt tgaaggggtg acctctgtca gcgacaaact gcacgttcgc
     3121 gacgctaaag aaggctcggt gaagggctac gcgggtgaca ccgccaccac cagtgaaatc
     3181 aaagccaaac tgctggcgga cgatatcgtc ccttcccgtc atgtgaaagt tgaaaccacc
     3241 gacggcgtgg ttcagctctc cggtaccgtc gattcccagg cacagagcga ccgtgctgaa
     3301 agtatcgcca aagcggtaga tggtgtgaaa agcgttaaaa atgatctgaa aactaagtaa
     3361 ttcgtcgtaa ttcgtcctcc cgaactttgt cgggaggcgc gatgtgcacc acactaaaaa
     3421 tatcgcattg agtgattttc acgctcatat caagcggtga cattaactat ggtaaaggag
     3481 acgcttatgt ttcgttgggg catcatattt ctggttatcg cgttaatcgc cgccgcactt
     3541 gggtttggtg gtctggccgg taccgctgca ggcgcagcta aaattgtctt tgtcgtcggg
     3601 attattctgt tcctggtgag tttgttcatg ggccgaaaac gaccctagat ttcagaccat
     3661 taagaaaatt ttatccaaag ccagtccagc ggactggctt ttgcggtttt agcgaataat
     3721 aaattgcgtt actttgtcat tctttcacaa caaaaacagg aaggcagagg tggggcagcg
     3781 aatacctgta acgcttggta atattgcgcc gttgtcgcta aggccgttcc agcctggacg
     3841 aatagctctg gtgtgcgaag gcggcggaca gcgtggaatt ttcacggctg gcgtgctgga
     3901 tgagtttatg cgcgcgcagt ttaatccttt cgatctttat ctcggcacat ctgccggggc
     3961 gcagaacctc tcggcgttta tctgcaatca gcccggttac gcgcgcaaag tcatcatgcg
     4021 ctataccaca aaacgcgaat ttttcgatcc attgcgcttt gtccgtggag gaaatcttat
     4081 cgatctcgac tggctggtgg aggccaccgc aagtcagatg ccgttgcaaa tggataccgc
     4141 cgcgcggttg tttgacagcg gcaaatcgtt ttatatgtgc gcctgtcgtc aggatgacta
     4201 cgcgccgaat tactttttac caaccaaaca aaactggctg gatgtgattc gcgcctccag
     4261 tgcgatacct ggcttttatc gtagcggagt gtcgctggaa ggcattaact acctggatgg
     4321 cgggatcagt gatgcgattc cggttaaaga ggcggcaagg cagggcgcta aaacgttggt
     4381 cgtcattcgc actgtgccgt cacaaatgta ctacacgccg cagtggttca aacgcatgga
     4441 acgctggctg ggtgacagta gcctgcagcc gctggtcaat ctggtgcagc atcatgaaac
     4501 cagctatcgt gacattcagc aatttattga gaaaccaccg ggcaagctgc ggatattcga
     4561 aatttatccg ccgaagccat tacatagtat cgcgcttggc agtcggattc cggcgctgcg
     4621 cgaagactat aaacttgggc gtttatgcgg tcgttatttc ctcgccacgg tcggtaaatt
     4681 attgactgaa aaagcgccgc ttacccgcca tctggtgcca gtggtgacgc cggaatcgat
     4741 tgtcattccg cctgcgccag tcgccaacga tacgctggtt gccgaagtga gcgatgctcc
     4801 gcaggcgaac gacccgacat ttaacaatga ggatctggct tgatttgccg ttttatcgac
     4861 acccactgcc attttgattt cccgccgttt agtggcgatg aagaggcctg cctgcaacgc
     4921 gcggcacaag cgggtgtagg caagatcatt gttccggcga cagaggcgga aaattttgcc
     4981 cgtgtgcagg cgttagcgga aaaatatcaa ccgctgtatg ccgcattggg cttgcatcct
     5041 ggtatgttgg aaaaacatag cgatgtgtct cttgatcagc tacagcaggc gctggaaagg
     5101 cgtccggcga aggtggtggc ggtgggggag atcggtctgg atctcttcgg cgacgatccg
     5161 caatttgaga ggcagcagtg gttacttgac gaacaactga aactggcgaa acgctacgat
     5221 ctgccggtga tcctgcattc acggcgcaca cacgacaaac tggcgatgca tcttaaacgc
     5281 cacgatttac cgcgcactgg cgtggttcac ggtttttccg gcagcctgca acaggccgaa
     5341 cggtttgtac agctgggcta caaaatcggc gtaggcggta ctatcaccta tccacgcgcc
     5401 agtaaaaccc gcgatgtcat cgcaaaatta ccgctggcat cgttgttgct ggaaaccgac
     5461 gcgccggata tgccgctcaa cggctttcag gggcagccta accgaccgga acaggctgtg
     5521 cgggtgttgg acgtgctttg cgagttgcgc ccggaaccag aagatgagat tgccgaagtg
     5581 ttgcttaata atacgtatgc ggtgtttagc gttagtgggt agtttgccct tcgtaggcag
     5641 catccggcag tcggcgcata atgcctgatg cgacgcttgt cgcgtcttat catgcctaca
     5701 g
//
LOCUS       DAZGSP010000193         5683 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZGSP010000193 DAZGSP010000000
VERSION     DAZGSP010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.195"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(9..1505)
                     /locus_tag="ACHK74_004971"
     CDS             complement(9..1505)
                     /locus_tag="ACHK74_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="HFP0223637.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGS
                     GNGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPEAGGNSDV
                     QGGYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVG
                     SEKAKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDRLPPNVRKFDVD
                     VEQFHYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNE
                     EPQRQNQAKKAAYDVFYSFSMNRDRIQSDVLNKAAELISDMGNKIGDYLGESYKSLAR
                     EIADDIKNFQGKTIRSYDDAMESLNKILSNPSFKVNRADRDALVNAWRSRDAQDIANK
                     LGNISKAFKAADVIMKIEKVKEKSIEGYETGNWGPLMLEVESWVLSGMAASVAMTLLS
                     AIIGSFTLPALALTAINIAGIMGISYLASYIDASVAEKINNKLVHFTH"
     gene            1744..2034
                     /locus_tag="ACHK74_004972"
     CDS             1744..2034
                     /locus_tag="ACHK74_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223638.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLA
                     DQRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
     gene            complement(2026..2304)
                     /locus_tag="ACHK74_004973"
     CDS             complement(2026..2304)
                     /locus_tag="ACHK74_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223639.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNAR
                     QKMFSNRLKIQLQIDELRASLAGTRDAQKREDLRKKLESLERQKKSLR"
     gene            2425..2847
                     /locus_tag="ACHK74_004974"
     CDS             2425..2847
                     /locus_tag="ACHK74_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFP0223640.1"
                     /translation="MRSAVARLLLIPLITATIVLTGCTPKTSLERHTRHYVYASDDGF
                     DPNFYTQKADTIRMMLPFFQQFRDMGVKDKAAGVSAETAQQRIKEFHSEKFFHSLRST
                     TTFAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
     gene            3245..4363
                     /locus_tag="ACHK74_004975"
     CDS             3245..4363
                     /locus_tag="ACHK74_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0223641.1"
                     /translation="MTKLYIYLVLAASLSALSTDMIVPALPELQKAFHADYSLIQLSI
                     SGFLIVFAFSQLISGTIGDYFGKVRVMTICLIIFLIGSIVCFMANDLNMLLLGRILQA
                     IGAGAGPVISKAIAKESFPPLKLKRALSDISSTSAIIPLIAPLAGALILDYYNWNVIF
                     IVMGAFSILTILLSPKETNKQEKKTHQDNVKFVTKDFICGTILVSLMLSSLFCYISIS
                     PALFMSDYGLNTFHYSVVFSLSVLFFIIGNQLSKIEKISNPWVLFPLNAISVTPFLIL
                     NDAFIICVIGAMIFNLTLGAYYPIANFISLQINGSRTGLAASITGFTQTVSAGTISFL
                     SVKISDNGVFLGTTLSISCLILALLSMFVTIIIGNNKK"
     gene            4360..5583
                     /locus_tag="ACHK74_004976"
     CDS             4360..5583
                     /locus_tag="ACHK74_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP0223642.1"
                     /translation="MNWQKKKIERSIKYQTRVFNEHINELVITRRNGKVITVNDGREM
                     IEFVSCSYLGLETHENLKHAIANAIERFGVQLSVARTRVKVDLFEQLEKRLNKIMNGA
                     HTLTFNAVTPCHLAVLPLLASGTLPNYKFNKKPYFILEKTAHATLQINRGLLQQFGET
                     IRVDFQHKNDIEEAFKYASINNMVPISISDSVGSMGGVAPVEFIVRMANRFDGYAYID
                     DAHGMSIYGKNGSGYVMSCLDNKLDDRIIIAGSLSKAFGSHGGFIACNYPDTINFIKT
                     YGTTYAFGGPPSLPGIAACIAAADLHLDGTVSLRQNDLQQVIKYFDDKFNETEVINKG
                     TTMPIRGLLIGNEEKAITICKKMHDRGFATTVAMYPTVEEGKAILRCAISAIHTRENI
                     DSLHKNFIECINEQG"
BASE COUNT         1784 a         1223 c         1004 g         1672 t
ORIGIN      
        1 tttttctatc aatgagtaaa atgaaccaat ttattattta ttttttcagc cacacttgca
       61 tcaatatatg atgcaaggta actaattccc ataatacctg caatatttat tgcagttaat
      121 gctaacgccg gtagtgtgaa tgatccaatt atagcactaa gcaatgtcat ggcaacgctt
      181 gcggccatac cactgagaac ccaagactca acctccaaca tcagcggccc ccagtttcca
      241 gtttcatatc cctcgatgct tttctccttg accttctcaa tcttcataat tacatcggca
      301 gctttgaaag ccttactgat attccccagt ttatttgcta tatcctgagc atcacgactt
      361 cgccaggcgt tcaccagtgc atcacggtct gctctgttaa ctttaaaact aggattagac
      421 aaaattttat ttaatgactc cattgcatca tcgtagctac ggatggtttt cccctggaaa
      481 tttttgatat catcagcaat ttctcttgct aacgacttat aagattcacc aagataatca
      541 ccgatcttgt tacccatatc agatataagt tcagctgctt tatttagcac atcactctga
      601 atccggtcac ggttcatact gaaagaataa aatacatcat aagcagcttt tttagcttga
      661 ttctgtcgtt gaggctcttc attattaaat cctaatgcct cgtatatctc ttccggggtc
      721 ttatggtcga ctttatccat aataccagcc ttagcttttt cactccctac ataaggacga
      781 acagctgttc tagttacaga taaaacatta ccataatcat caagcaccac taaatagtgg
      841 aactgttcaa catcaacatc aaattttctc acattaggag gcaaacgatc ccacgcatct
      901 tcagcctgtt gttttgcttt ttcctgccgc tgagattcat ctttattaaa acctaacgcc
      961 tcatatatct cctcaggtgt tttatgattg attttttcca taatacccgc cttagctttt
     1021 tcactcccta cgtaaggacg gacacctgtt cgaagaatac cgatagctct accattatca
     1081 ttaagcgtta cacgatattt aaaaccatcg atactcacat caatctctcg cggtaagttg
     1141 tttttatccc acttacgagc aacttcatca ttaggatttt caggaatata gcccccctga
     1201 acatcactat ttccgccagc ctcaggaact ccatatacag gcgtttttac cagaccattt
     1261 gtaatggtaa tgcgcacctg cccattatca tcaacataag gcggcacatc cggattattg
     1321 gttttactcc atccccaccc cgtctgactt ccatgttcac cacctccatt accagagcct
     1381 gaaccaccac cccagtgaac cccactatta ctattattat ttccaccttg ctgattcaga
     1441 ttcgcccctg ttccccccat agactcacca gcagttgggc catatccact tagttcttta
     1501 gccataaatt cctctttgat aattaaaaca ataaattaaa aacaacatac tgtacatata
     1561 accactggtt ttatgtacag caaaacctac tactcaccat tgtccatgtc aagagcatgg
     1621 attttcattt ttgcaataag gatcacacta tggggaggca ggcattgaga acgtcgaaac
     1681 agaacaccgg agcaaatcag gatgagatat aaaactgttg gatcatgaaa aacggagaac
     1741 gatgtgagca aatcaccccg ccataaactg aacaaaacag acaaacgact tctcgacacc
     1801 cttgttgctg ccggatatga gcatgacaaa gcccgtgacc tcatccagaa acaggtttac
     1861 acgctgacac tggctgatca gcgccatgtg gtcagtgaaa tcagtaacgg tgtgaatccc
     1921 acccaggctt actcggcggt ataccaggca agacgcattc gcctcgcccg taaatatctg
     1981 aacggtaaaa aggttatgga agaaaccggg gaaaatacgc ccccatcagc gtaaggattt
     2041 cttttgccgc tccagagact ccagtttttt acgcaaatcc tctctttttt gggcatctct
     2101 ggtgccagcc agagatgccc tcaactcatc gatctgaagt tgtatcttca gtctgttact
     2161 gaacattttc tgtctggcat taacatccgc aacaatgcca ttttttgtct tctcggcctt
     2221 ttgttgaaaa acattgctgt ccgcatgact ggcaaccgaa gcagaaagaa cactaaaaag
     2281 caggactggc acacattttt tcacgggatt attcctgact cattgaccat caaatcacat
     2341 tggggtaaac cgacgtatga taagagatac tcttcggaaa tataactccc tgagtatcaa
     2401 gattaaaaac gcaaggagat gtttatgaga tctgccgttg ccaggctgct tctgatacct
     2461 ctgataacag caacaatcgt tcttacagga tgcacaccaa agaccagcct ggaacgacat
     2521 acccggcatt atgtttatgc ttcagatgat ggatttgatc ctaacttcta cacccagaaa
     2581 gcagacacca tacgtatgat gctcccgttc tttcagcagt tccgggatat gggggtgaaa
     2641 gacaaagcag ccggagtatc agcagaaacg gcacagcaac gtataaaaga attccactca
     2701 gaaaaatttt ttcactcact ccggagcaca acaacctttg ctggcagaaa atacacaaac
     2761 agcgatatgc cgtcgccgaa aaaaatgaaa ctaatggcag acaccatttc tgcggtttat
     2821 ctcgatggat acgagggcag acagtaaggg gatttaccat aatcccttaa ttgtacgtac
     2881 cgctgaaacg ctctcatacg cgttctgacg caaaaacgca aaaagacaag gaaaccccag
     2941 gctaaaccga aaaaatgcgt cagaatcgtt ttcaggtact tgcataacta ttcacaaaag
     3001 cgatgcgcga tcgatttcga ctattccgac ggacgaacag gttaaaatga taagtttcct
     3061 ccctccggat ggaaggttca gcacggggat ttttaccccc ttccatcaac ctgaatatgc
     3121 atctgggagt acccattagt ttctactcaa acactatcgt agtgctatct caaaatataa
     3181 aacataccga tggcgtaagc attttaaata tgataaataa gaatattttt atcagggatt
     3241 aattatgaca aaactatata tttaccttgt tcttgctgca tcattatcgg cattatcaac
     3301 tgacatgatt gtgcctgcgt taccagaact acagaaagca tttcatgctg actactcatt
     3361 aattcaacta agtattagtg gatttcttat tgtatttgca tttagtcaac tgatatcagg
     3421 aaccataggt gattatttcg gaaaagtacg cgtaatgact atatgcttaa ttatatttct
     3481 tattggcagt attgtatgtt ttatggccaa tgatttaaat atgcttcttt tgggtagaat
     3541 tctacaggca ataggtgcag gtgccggacc tgtaatatca aaagccattg ctaaagagtc
     3601 ttttccacca ctaaagctaa aaagagcact ttctgacata tcatcaacaa gtgcgataat
     3661 accattaata gcacctctgg ccggagcatt aatacttgat tattataact ggaatgtaat
     3721 atttattgta atgggtgcat ttagcattct aaccatactc ctgtcaccta aagagactaa
     3781 taaacaggag aaaaaaacac atcaagataa tgtaaaattt gtgactaaag atttcatctg
     3841 cgggacaata ctcgtttcac taatgctaag ctcactattt tgctacatat caatatcacc
     3901 tgcactattt atgagtgatt atggattaaa tacatttcac tactctgttg ttttttccct
     3961 gtcagttttg tttttcatca ttggaaacca attgtcaaaa atcgaaaaaa tatcaaaccc
     4021 atgggtctta tttcccctaa atgcaatatc agttacacct tttcttatat taaatgatgc
     4081 ttttataata tgtgttattg gagcaatgat tttcaattta actcttggcg catactatcc
     4141 aatagctaat ttcatctcat tgcagataaa cggttcacga actggtttag cagcatcaat
     4201 tacaggcttt actcaaacag taagcgcagg gacaatcagt ttcctttcag taaaaataag
     4261 cgataatgga gtttttttgg gaaccacttt aagtattagt tgtcttattc ttgcattatt
     4321 gtcaatgttc gtaactatta ttatagggaa taataaaaaa tgaattggca aaagaaaaag
     4381 atagaacgtt caatcaaata ccaaacaaga gtatttaacg aacatataaa tgaactagtg
     4441 atcaccagaa gaaatggtaa ggtaatcact gttaatgatg ggagggagat gattgagttt
     4501 gtgtcatgct cttatcttgg actggaaaca catgaaaatc taaaacatgc aatcgctaat
     4561 gcaattgaaa gatttggagt ccaactttcg gtcgccagaa cgcgcgtaaa agtagattta
     4621 ttcgagcaat tggaaaagag attaaataaa ataatgaatg gggcgcacac attaacattt
     4681 aatgcggtta ctccatgcca tttagctgta ctcccacttc ttgcatcagg aacactacct
     4741 aattacaagt ttaataaaaa accatatttt attttagaga aaacagcgca tgcaacattg
     4801 caaataaata gaggactatt acaacagttc ggtgaaacaa tcagggttga ctttcaacat
     4861 aaaaatgata tagaagaagc gtttaaatat gcttcaataa ataatatggt accaatatct
     4921 atatctgata gtgtcggttc gatgggtggg gtggctcctg tcgaatttat tgttagaatg
     4981 gctaacagat ttgatgggta tgcttacatt gatgatgctc atggcatgtc aatatatggg
     5041 aaaaatggct ccggatatgt aatgtcttgt ctggataaca aacttgatga cagaattatt
     5101 atcgctggtt cattatctaa agcgtttggc tctcatggtg gatttattgc atgcaattat
     5161 cctgacacca taaactttat aaaaacgtat ggaactacct atgcttttgg tggtccacca
     5221 tcattaccag gaattgctgc atgtatcgca gcagcagatt tacacctcga tggcacagta
     5281 tccttgcgtc aaaatgactt acaacaagtt ataaaatact ttgatgacaa atttaatgaa
     5341 accgaagtca taaacaaagg gactacaatg ccaatacgtg gattgttaat tggcaatgaa
     5401 gaaaaagcta taacgatatg caaaaaaatg catgatcgtg gttttgctac aaccgttgct
     5461 atgtatccta cagtggaaga gggaaaggca attctacgtt gtgcaatatc agctattcat
     5521 acccgagaaa atatagatag cctacataaa aactttatag aatgtattaa cgaacagggt
     5581 tagtaaatat catggcctct ttatagagag gccatctatt tcattttttt atggcgtata
     5641 gctgtccctg acattacata ctcatgcccc ggtagtgtcc ggg
//
LOCUS       DAZGSP010000194         5548 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZGSP010000194 DAZGSP010000000
VERSION     DAZGSP010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.196"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..357
                     /locus_tag="ACHK74_004977"
     CDS             <1..357
                     /locus_tag="ACHK74_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFP0223643.1"
                     /translation="QLYPQVQWVQSFADERCGCLGVVYQASNFDYVGSHESTFYELDG
                     EWYHEICRNAIKRGGQRGEHLRANIDRASVHKFRQFRYIRFLNKRARKRLNTKLFKVQ
                     PYPKPQTVKTGLKESE"
     gene            350..1645
                     /locus_tag="ACHK74_004978"
     CDS             350..1645
                     /locus_tag="ACHK74_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HFP0223644.1"
                     /translation="MSERLKTHSKTQKKSADLVSNKLRIGVFQRYQMKCKSVSKKSPR
                     YNKQLRAANKVTNVLDTELTSARAAERKAQQTAQQGMKEEERELCASHFPVVTLEQLP
                     DIRDLERQHERGIQHEIERVKAELHRLNIELTKRMSEAKRVDTGALVEAGADLDDITV
                     YLQRLQELTEEALPEKLNRFLDYLNRSSDDGVTQLLSHIEHEVLVIEERLNELNETMF
                     RVDFQPYRYLRLDTKKVVHESLRTLEKAQRQLNAARFVDDNGESYYKALQILVAQLRD
                     ACERNRTLGAKALLDPRFRLEFAVSVMDRQSGNVIESRTGSQGGSGGEKEIIASYVLT
                     ASLSYALCPAGSRYPLFGTIILDEAFSRSSHAVAGRIIAALREFGLHAVFITPNKEMR
                     LLRDHTRSAIVVHRRGQNSNMASLSWEELERHYQRRGNA"
     gene            1647..2795
                     /locus_tag="ACHK74_004979"
     CDS             1647..2795
                     /locus_tag="ACHK74_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3322 domain-containing protein"
                     /protein_id="HFP0223645.1"
                     /translation="MYSPDELREKLARQWDSAKLRAERLLSPGNWPLCLPIGKPSTKI
                     FAEQTQRVLQHVQRWRQVAVGHVEWEAVSYRASDTPVLMPLRWILNSPSEWINAAADP
                     VVSREFRLLEGIIEQVNPIFHPLLVKHRSLWRHKDPQGVISAATLACRLEPGCAKGLP
                     LRLLSGQGVDTKFIENNISLLTRLLDVRFSGEASEQGLTTFLDAFDESSHWVLVVPLS
                     PGLLPFKKCRVTTAELAETTLPASQVLVVENEQCLHHLPALSDTIAILGCGLDVQWLS
                     SSVLDEKRVAYWGDMDSWGLLMLARARRCRPTLDALLMNRELFEQYASHSAVPEPVIA
                     KEAVPDGLLNEEADFYRYLTRLPCGRLEQEFLPVGIAEEVLLRWGKES"
     gene            complement(3349..4191)
                     /locus_tag="ACHK74_004980"
     CDS             complement(3349..4191)
                     /locus_tag="ACHK74_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP0223646.1"
                     /translation="MSDITVSRPEVVNWHTDVICSTSILHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQNFHCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLMRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHVQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPG
                     ALAI"
     gene            complement(4276..4473)
                     /locus_tag="ACHK74_004981"
     CDS             complement(4276..4473)
                     /locus_tag="ACHK74_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP0223647.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLHQNR"
     gene            complement(4485..4973)
                     /locus_tag="ACHK74_004982"
     CDS             complement(4485..4973)
                     /locus_tag="ACHK74_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP0223648.1"
                     /translation="MKLTLTLEADSVNVQTLNMGRIVVDVDGVNLAELINVVCDNGHS
                     LRVVDESDRVSTDCTPPFAALTGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(4970..5347)
                     /locus_tag="ACHK74_004983"
     CDS             complement(4970..5347)
                     /locus_tag="ACHK74_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP0223649.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFNACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITQGKHPEAKQ"
     gene            complement(5394..>5548)
                     /locus_tag="ACHK74_004984"
     CDS             complement(5394..>5548)
                     /locus_tag="ACHK74_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP0223650.1"
                     /translation="LMLTSGELNPRNQHTVTLYAKGLTCEADTLGSCGYVYLAVYPAQ
                     APATTS"
BASE COUNT         1371 a         1253 c         1457 g         1467 t
ORIGIN      
        1 cagctttatc cgcaggtaca gtgggttcag tcgtttgcag atgagcgttg tggctgtctt
       61 ggtgtcgtgt atcaggcaag caattttgat tatgtaggca gccacgaatc aacattctat
      121 gaacttgatg gcgagtggta tcacgagatt tgcaggaatg ccattaaacg aggcggacag
      181 cgaggtgaac atctgagggc taatatcgac agagccagtg ttcataaatt ccgtcagttt
      241 cgttacatcc ggtttctgaa caagagggcc agaaaacgcc tcaacacaaa gctattcaaa
      301 gtccagcctt acccaaaacc acaaacagtt aaaactggtt taaaagagag tgagtgaacg
      361 cttaaaaact cattctaaaa cccaaaagaa aagcgccgat ttagtatcaa ataagttgcg
      421 tatcggcgtt tttcagcggt atcaaatgaa atgcaaatcg gtatcaaaaa aatcgccgcg
      481 ctataataag cagctcagag ctgccaataa agtcactaac gtgctggata cagaactcac
      541 atcagcccgt gccgcggaac gtaaggccca gcaaactgca caacaaggta tgaaggaaga
      601 agagcgagag ttgtgcgcgt cacattttcc agtggtgact ttggagcaat tgcctgacat
      661 tcgcgattta gaacgtcaac atgagcgggg aattcagcat gaaattgaaa gagttaaagc
      721 tgaactacat cgactgaaca ttgagttgac caaacgtatg tctgaagcca aacgtgtgga
      781 taccggggcg ctcgttgagg caggggctga tttggacgat attactgttt atttacagcg
      841 tttgcaggaa ctgaccgaag aagccctgcc ggaaaagctc aatcgcttcc ttgattatct
      901 taaccgttca tccgatgacg gcgtaacaca actgcttagc catattgagc atgaggtgct
      961 ggtgattgag gagcgcctca atgaactcaa tgaaactatg ttcagggttg attttcagcc
     1021 gtatcgctac ctgcgtctgg ataccaaaaa agttgtccat gaaagcctgc gcacgcttga
     1081 aaaagcacag cgtcagctaa atgccgcccg atttgttgat gataatggcg agagctatta
     1141 taaggctttg cagatactgg tggcgcaatt gcgggatgct tgtgaacgaa accgtaccct
     1201 gggagcaaaa gcgctactgg atccccgttt tcgactggag ttcgccgtat cggtgatgga
     1261 tcgtcagagc ggaaatgtga ttgagtctcg taccggctca cagggtggta gcggtggaga
     1321 aaaagagatt atcgcttctt acgtgttaac agcttcactt agctacgcac tttgtcctgc
     1381 ggggagccgt tatccgcttt tcggtaccat tatccttgat gaagccttct cccgcagttc
     1441 tcatgcggtg gcgggtagga tcattgccgc gcttagagaa tttggcttac atgcagtatt
     1501 tatcaccccc aataaggaaa tgcgcctgct gcgtgaccat actcgttcgg cgattgttgt
     1561 ccaccggcgc ggtcagaatt ccaatatggc ttctctgagc tgggaagagc ttgaacgaca
     1621 ctaccagcga cgggggaacg cgtaggatgt attccccaga tgaattgcgt gaaaagcttg
     1681 cccggcagtg ggatagcgca aaacttcggg ctgaacgctt actgtccccc ggtaactggc
     1741 cgctctgcct gcctattggt aaaccttcca cgaaaatatt tgcagagcag actcagcgcg
     1801 ttttgcagca cgtacagcga tggaggcaag ttgcggttgg gcacgtggaa tgggaagcgg
     1861 tgagttatcg ggcaagtgat acaccagtat taatgccgtt gcgctggatt ctgaatagcc
     1921 cttctgagtg gatcaacgcc gctgcggatc cggttgtgag ccgggaattt cgcttgctgg
     1981 aaggaattat tgaacaagtt aatcccattt ttcacccgct gttggtaaag catcgctctc
     2041 tctggcggca caaagatccg cagggcgtca tcagcgccgc cacgctggcc tgccgattag
     2101 agccgggctg cgcaaaaggg ctgccgttaa gattgctttc cgggcaagga gtggatacca
     2161 aatttatcga gaataatatt tcgttattaa cccgactgtt agacgtgcgg ttttcaggag
     2221 aagccagcga gcagggatta acaacttttc ttgatgcttt tgatgagtcc agtcactggg
     2281 ttttagttgt accattatca cccggcttac ttccttttaa aaaatgccgg gtaacaacgg
     2341 cggaactggc agaaacaaca ttacctgcgt cgcaagtact ggtggttgaa aatgagcagt
     2401 gtctgcatca tcttccagca ttatctgaca ctatcgcgat tttaggctgt ggcctggacg
     2461 tgcaatggct atccagttct gttctggatg aaaaacgcgt tgcttattgg ggggacatgg
     2521 atagctgggg attactgatg ctggcgagag ccaggcgctg tcgtccgacg cttgatgccc
     2581 tgctcatgaa ccgtgagttg tttgagcagt atgccagtca tagcgccgtt ccagaaccgg
     2641 tcatagcgaa ggaagctgta cctgatggct tgcttaacga agaggccgat ttttatcgct
     2701 acttaacccg tttaccgtgt ggtcgtctgg agcaggagtt tttgccagtg gggattgcgg
     2761 aagaggtgct tctcaggtgg ggaaaagaga gttaaaaaag tcatgcttgc tctggaaggt
     2821 tatttacgcg gtattttgta gtggttgttg cgaaagtctg cgttgatggt cctgatacct
     2881 taccgggtag agggactttc catcagtcag tttagatact gtggcaactg tcgcctttag
     2941 gtggacaaat gcataaatta ttaaactatt aaatttcttg ttttcaggca gcagagagaa
     3001 ggtatatcag agggtactga ggtgtataaa cgtcacgaac atccgtgaaa aggcgaaaaa
     3061 aaccagccaa tagataatgc tttgcaatgg ccagaaatct accgtagcca gaaagtgaaa
     3121 aagccccgct ttgcagcgag gccttatgaa tagtggtgcc cggactcgga atcgaagatt
     3181 tcatttatca tattgatatt aatctcgttt atttggtttg atgaaaaatg taccctctct
     3241 tgtaccctcg gtcccttctg gtggttttta tgagaacaag tagatcaaga tttttgtcat
     3301 cagaacacgt gtatttttgt gggttttatt cctgagtttt attccttttt atatcgctaa
     3361 cgctccggga tagtgcttag caataatatc attaagcctg tcaaccagct ctggtttcct
     3421 gaacgtgatg tgcgcggagc ccttcttaaa gtatctgatg ctgaacatct catcttcata
     3481 gtcctcttta ccctgaacag actggacgtg atcatccaga tgaatagtga tgttttcccg
     3541 gttgtcgggg atcggtttgc cgctgaacag gtgtagcatt ctttccagat cagcaagtcg
     3601 atctgcctgt tgcccggtga taagatgaaa gccccaccgg tcccacctca tcaggttgtt
     3661 gacgataatt ttactgccaa atttgcaggg gcaattggtt ttgtaattcc agctcaaccc
     3721 tctgaagacg ttgatcaccc cccgctcaaa cacctcatcc ttattctggt gcagttgttc
     3781 aaatgtgctc agaatgttcg cttcactgat ttccggaaaa ttatcgtatt ccagtgaccg
     3841 gtaccatgta tcacgggcct gtgcatccat taaggaaagc atcccggaac gttgcatcag
     3901 gtcacgccag atttcgcgat cgagattgcg tgtgatggct ttcattgcgg tttcaggttt
     3961 ctccatcagc cagcaaccgc agtgaaaatt ctgtttcatg gcccagtcca gtgcggtttt
     4021 accgccaatg ctttctgtca ttgctgagat ttcagccagt tgccggataa gtgtgtcgat
     4081 ttgcagcagt gtactctttc gtacagccag aatgtggagg atactggttg agcagataac
     4141 gtccgtatgc cagttgacaa cttccggacg ggagactgtg atatcagaca taatcgatat
     4201 tcctttttgc agataaatga aaacgccagc cctgttcagg ctggcgtcag tggaatgaag
     4261 tctggtgagt gagagtcacc ggttctggtg cagaagctgc aactggcgca gtgtacggat
     4321 atcttccctg gcctgttcaa tgcagggcag cagtgctgcc gaatccgttt tatcctcatc
     4381 gttgtcaaag ataattcccg attcgcagtc gatattgtcc tgcagccacg caatcagaat
     4441 atccagcgct gtttccgtgg ttaatgattt catgttgttt ctggttacca gttgaaagtg
     4501 ggtaaacccg gcaggcattc agcgctggca tccagatgaa tgagactgac gccataacgt
     4561 cgggtaagtg tgacaaccag acggcggaac gtttttgaca gccccaggcg tttaagccgc
     4621 agaaccgggt atgaccacgc atcggtacgt aacagatatc cgctacctgt gaaatgaatc
     4681 cattctgatt cgccggtgtc attcgtctga tgtgacagcg agtacagcca ggcgttgtcc
     4741 tttgcagtga tatgcgcggt actgcagcgt atgccggtca gggcagcaaa tggtggcgtg
     4801 cagtctgttg agacccggtc agattcatca acaacacgaa gggagtggcc attgtcgcag
     4861 accacgttaa tcagttcagc gagattaaca ccatcgacgt caacgacaat tcgtcccatg
     4921 ttcagcgtct gcacgttaac gctgtctgct tccagcgtca gagtcagttt cattgtttcg
     4981 cctccggatg cttcccctgg gtaatgttat ttaccgttct gtaattgtcg cgggtcatca
     5041 ggccggtcgc cctgcgagcc cggaggatat cgatgctgtt tattaactga gaacgggtac
     5101 aggcattgaa ttccggctgg tcggtacgca ccagtgcgta tttttcaaca agaaagttca
     5161 ctgcatcgca cagtgaaatg ccagcttcaa tatgctgctc aatcacacgt tcatcggcaa
     5221 acggtgtgtc attcagtgtg aggccataat gctggtccag cagtcggatg agcagtgtct
     5281 gccagatggt gacgggagac gggcagcgag atacctcccg tacatgtgtg tcggataatg
     5341 ttttcatctt aagttctctg taactggtag tgctaaaggt gagagcttcg gggttatgag
     5401 gtggttgcgg gtgcctgtgc cggataaaca gccagataaa cgtaaccaca ggagccgagg
     5461 gtgtcggctt cgcaggtcag cccttttgca tacagcgtga cggtatgctg atttcgggga
     5521 ttcagttcac cgctggtgag catgagtt
//
LOCUS       DAZGSP010000195         5443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZGSP010000195 DAZGSP010000000
VERSION     DAZGSP010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.197"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(165..317)
                     /locus_tag="ACHK74_004985"
                     /pseudo
     CDS             complement(165..317)
                     /locus_tag="ACHK74_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
     gene            654..2210
                     /locus_tag="ACHK74_004986"
     CDS             654..2210
                     /locus_tag="ACHK74_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012884439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFP0223651.1"
                     /translation="MHDYLLFLLGLTPLLAMIILILKVKMPIHYSVLSTLLLTLILGG
                     VFWQTPVMDMGSAVVYGAIKGLWPIVIVILAAIYSYNLMNATKSMDILRNVLASVSDD
                     KRIQVLLISWCFGGFLEAAAGYGTAVAIPIGILIALGFNPLKAAIASLVANTVPTAFG
                     AVGIPVSVLAEQVNLPVTTLSGTIILQLALFNILLPFVIVTIIGGGMKAIRGVGLITL
                     MCGISTLIPQYLVAVNLGAELPAFAGSLVSLIVIVFMAKNRKEKTEPEFLIESSADSA
                     SALSYSRGALLRACSIYILIFLFILLCSPLFPTIKSAVSQIASSLTFSLSNGSNLSMK
                     IEWIATPGVLIIIASVIGGLIQKASLSCLLNVFVSTVKQLKNSIIAISAIVAMATVMD
                     VCGLIGTLAQSLVDLTGGAYLFIAPVIGALGTFVTGSDTNSNILFGKLQTTAAEKLHV
                     DPLWLAAANTSGATGGKMISPQSIAIAVSATRMDGQGSVIMSGTLKYCFAYIVILGLK
                     VGLVYHLFMA"
     gene            2260..2979
                     /locus_tag="ACHK74_004987"
     CDS             2260..2979
                     /locus_tag="ACHK74_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFP0223652.1"
                     /translation="MNVNFFVTCIGDAFKSRMAQKSVLLLEQLGCNVHFPEKQGCCGQ
                     PAINGGYVRDAIPGMKNLISSLEENDWPVVSPAGSCTYTIKNYARYLSEEPEWAARAE
                     KVAERLHDLTSFIVNHLGITDVGAKLNGRAVYHPSCSLSRKLGVTREPLLLLENVQGL
                     ELLPFHAQETCCGFGGTFSVKMAEISGEMVKEKVGHIMDVKPDFLIGADASCLLNIGG
                     RLNREGHPVKVMHIAEVLMHR"
     gene            2990..4405
                     /locus_tag="ACHK74_004988"
     CDS             2990..4405
                     /locus_tag="ACHK74_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFP0223653.1"
                     /translation="MSLKTSDIRFKERIRIQIQDPIMRKAVANAQERIGTNRQKMVDE
                     LGHWEEWRSRAEQIRRHVLENLDAYLYQLSEKVTANGGHVYFARTREDATSYILKVAK
                     EKNARKVVKSKSMVTEEIGMNAVLQKAGIPVIETDLGEYILQLAHEPPSHVVVPAIHK
                     DRHQIRRVLHEHLGYDGPETPEAMTLFIREKIRQDFLSAEVGVTGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFQEVDVLITMLARSAVGARLTGYNTWLTG
                     PREEGHVDGPEEFHLVIVDNGRSEILGSEFSDVLRCIRCGACINTCPAYRQIGGHGYG
                     SIYPGPIGAVISPLLGGYEDFKDLPYACSLCTACNGVCPVKIPLATLIQKHRQVMAES
                     EITPLTERRITRAFNYINSHPALWKVGMVAGAKTARLFIRKGKTPISVGAIKEWTEAR
                     DLPSPDGESFRSWFKRHKKEG"
     gene            4409..5107
                     /locus_tag="ACHK74_004989"
     CDS             4409..5107
                     /locus_tag="ACHK74_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFP0223654.1"
                     /translation="MENREEFLVAIARKLGRPVRHIPEAMPEPVNTLATTRLTELTSD
                     QRCEAFIKFASEVMLAECVLVSPENAPSTALDICWKFGSGPVIISNDQRLVDTGITPL
                     LQKEMDAALWDPEKGDENIHLAEKAKTGVVFAEYGLTESGGIVLFSAPERGRSVSLLP
                     EASLFVLRKSTILPRVAQLAQKLHDMVQEGQRMPSCINLIGGPSSTADIELIKVVGVH
                     GPVHATYLIIDDCD"
BASE COUNT         1463 a         1172 c         1306 g         1502 t
ORIGIN      
        1 tccaactata taacatattt tttgtcatgt tttttataaa actttaaatt aattgcaaaa
       61 tggcatctga atcgatcatc gtttggcatt cgaatcgatc atgatttggc tctcttgctc
      121 cggctttatc cacttcctct cagacgacag tcagtgtcct gctctgactg aacctctgca
      181 ccgttgttac gtaccagcgt ttgtttatct attagagtca cttcagtagg cacatcagat
      241 gccaacgcaa cattccctgc cataagcgca gccagaacgc ctgttacaac taaactattc
      301 ctggtgatat tgctcattct ctttctccat ttgtcgagga tttgggcgtt gattttcgac
      361 gagctccagt tgcaactcca gtcttaaggt tatatcggga tagcacacaa gtgttacacg
      421 cataaacaaa aaatacccaa aaagtattat ttcataacat ttattcatgg aagataacaa
      481 tgaaacgatt cagctatatt ttttaaacta aaaaatactt tataagaaca tcccaatttt
      541 ggaggcgata ctcattctcc ccctgataaa taaacactct cgtccgacag ggcgtggaaa
      601 ctatcaataa ctacagtaat gatgctgact ttgttttcgt ttaaggaaat gacatgcacg
      661 attacctgct ttttttactg ggacttaccc cccttctggc gatgatcatt ttgattctga
      721 aagtcaaaat gcccatacac tattctgtgc ttagcactct cctgctcacg ctaatccttg
      781 gaggggtttt ctggcagacg ccagttatgg atatgggatc tgctgtggta tacggcgcaa
      841 tcaaaggttt gtggccgatt gtgattgtca tccttgctgc catttacagc tataacctga
      901 tgaatgcgac aaaaagcatg gatattctga gaaatgttct ggcgagcgtt agtgacgata
      961 aacgcatcca ggttcttctt atctcatggt gttttggcgg atttcttgag gcggccgccg
     1021 gatacggtac agcggtagca atccccatag gtatactcat tgcactgggc tttaacccac
     1081 taaaagcagc gattgcatca ctggttgcta atacagtccc tacagcgttt ggggcagtcg
     1141 gtatacctgt ctccgtgctt gctgaacaag ttaatttacc tgtaaccaca ctcagcggga
     1201 cgattattct tcagctggca ctgttcaaca ttctgctccc cttcgttatc gtcacgatta
     1261 tcggtggagg tatgaaagct atacgaggcg tagggttaat taccctgatg tgcggtatta
     1321 gcacattgat acctcagtat ctggtggctg ttaatcttgg cgcagagctc ccggctttcg
     1381 caggaagtct tgtcagtctg atcgttatag tctttatggc aaaaaaccgt aaagaaaaaa
     1441 cagaacctga atttcttatt gagagttcag cagatagcgc cagcgcactt tcatactctc
     1501 gtggggcgtt gttacgtgcg tgctcaatct acattctgat ctttctgttc attctgcttt
     1561 gctctccact gttcccgacg ataaaaagtg cagtgtctca gattgcctcg tcactcacct
     1621 tctctctgag taacggcagc aatctgtcga tgaaaattga atggatcgca acccccggtg
     1681 ttctgattat cattgccagt gttattggcg gcttgataca gaaagcatcc ctgtcatgcc
     1741 tgttaaacgt atttgtcagc acggtaaaac aacttaaaaa ctccattatt gccatttctg
     1801 cgattgttgc catggcaacg gtaatggatg tctgtgggtt gattggcact ctggcacaga
     1861 gccttgtgga tctgactggt ggggcctacc tcttcattgc tccggttatc ggcgctcttg
     1921 gtacatttgt tactggtagt gacacaaact ccaacattct gtttggtaaa ttgcagacta
     1981 ctgcggctga aaaattgcat gtggacccgc tctggctggc tgccgcaaat acatctggcg
     2041 caacaggcgg caaaatgatt tctcctcaga gtattgctat cgctgtttca gccacccgta
     2101 tggatggtca gggcagcgta atcatgtcag gaacactgaa gtactgcttt gcttatattg
     2161 ttattcttgg actgaaagta ggccttgtct accacctgtt catggcctga gaaaaaacaa
     2221 caaaaggacg tggaatatta atcaacagga gaaatttctg tgaatgttaa ctttttcgtc
     2281 acctgcattg gtgatgcgtt taaatcccgg atggcacaaa agagtgtgct gttactggag
     2341 caattgggtt gcaatgtcca tttccccgaa aagcagggat gttgtggtca gccagcgata
     2401 aatggcggtt atgttcgtga tgccatcccg ggcatgaaaa atctcatatc ttcgctggaa
     2461 gagaatgact ggccggtagt atctcctgcc gggtcatgca cttacacgat aaaaaattac
     2521 gctcgctatc tcagtgaaga acctgagtgg gcggcacgag ctgaaaaagt ggctgaacgc
     2581 ctgcatgatt tgacatcatt tatagtaaat catcttggca tcacagatgt tggggcaaaa
     2641 ctcaatggtc gcgctgtgta ccatccctcc tgcagccttt cccgtaagct gggtgtaacc
     2701 agagagccgc ttttgctact tgagaatgta caggggcttg agctactgcc gtttcacgct
     2761 caggaaacgt gctgcgggtt tggcggcaca ttctctgtaa aaatggcaga gatctctggt
     2821 gaaatggtga aagagaaagt cgggcatatt atggatgtta aacctgattt tcttatcggg
     2881 gctgacgcca gttgcctgct aaacatcggc ggtcggctta accgtgaagg gcatcctgta
     2941 aaagtgatgc atattgctga agtcctgatg cacagatgag gtaaacagaa tgtctttgaa
     3001 aacaagtgat atcaggttta aagagcgtat ccgtattcag attcaggacc cgatcatgcg
     3061 aaaagctgtg gctaacgcac aggagcgcat agggactaat cgacagaaaa tggttgatga
     3121 actgggacac tgggaggaat ggcgttcacg agctgaacag attcgtcggc atgttctgga
     3181 aaatcttgat gcttatttgt atcaattgtc cgaaaaggtt actgcgaatg gcgggcatgt
     3241 atattttgcc cgtaccagag aggatgcaac cagttacata ctgaaggtgg cgaaagaaaa
     3301 aaacgccagg aaggttgtta aatccaaatc aatggtgact gaggaaattg gcatgaatgc
     3361 cgttcttcag aaagccggga tacccgtgat tgaaacggac cttggggaat atattcttca
     3421 gctagcacat gagccaccat cgcatgtggt tgtgccggca atacataaag atcgccatca
     3481 aatccgcaga gttttacatg aacatctggg gtatgacggg cctgaaactc cagaagcaat
     3541 gacattgttc atccgtgaga aaatcagaca agattttctg agtgctgaag ttggcgtaac
     3601 ggggtgtaac tttgccgttg cagaaacagg gtcagtctgc cttgttacaa acgaaggcaa
     3661 cgcacggatg tgcaccacat tacctaaaac acatattgct gttatgggta tggagcgtat
     3721 tgctccgaca ttccaggagg tggatgttct gattaccatg cttgcaagga gtgctgtcgg
     3781 ggcgagactg acaggttata acacctggct tacaggcccc agagaagaag gacatgttga
     3841 tggtccggaa gagttccatc tcgttattgt tgacaatggg cgttcagaga ttctcggctc
     3901 agaattcagc gatgtattgc gatgtatccg ctgcggtgcc tgcattaata cctgcccggc
     3961 ttatcgtcag attggtgggc atggttatgg ttcaatctac ccggggccaa tcggcgcggt
     4021 tatttcccca ttgcttggtg gatatgaaga ctttaaggat ttaccctatg cctgctcatt
     4081 gtgcactgcc tgcaatgggg tatgtcctgt aaaaatacct ctggcaacgc taatacaaaa
     4141 acatcgccag gttatggcag agtctgaaat cacaccatta acggaacgcc gtataaccag
     4201 agcattcaat tacataaaca gccatccggc gttgtggaag gtaggtatgg ttgctggggc
     4261 caaaacagcc cgtctgttca tcaggaaagg aaaaacgccg attagtgttg gagcgataaa
     4321 agaatggact gaagccagag acctgccatc acctgatgga gaaagcttcc gtagttggtt
     4381 caagcgacat aagaaggagg ggtaatttat ggaaaacaga gaggaatttc tcgttgcgat
     4441 cgccagaaag cttggcagac cagtaagaca tattcccgaa gcaatgccgg agcctgtaaa
     4501 tacgctggct acgacacgtt taacagagtt gacatcagac caacgctgtg aggcgtttat
     4561 taaatttgcc tcggaagtga tgcttgccga gtgtgttctg gtgtcgcctg agaacgcacc
     4621 atcaacagcc ttagacatct gttggaagtt cggttccggg ccagtcatta tcagcaacga
     4681 tcaacgactg gtcgatacag ggattacgcc attgctacaa aaagaaatgg acgctgctct
     4741 gtgggatccg gagaagggcg atgaaaatat ccatcttgct gaaaaggcaa aaactggtgt
     4801 cgttttcgct gagtatgggc tgacagaatc aggaggcatt gtcttattct ccgcaccaga
     4861 acgtgggcgc tcagtaagcc ttttgcctga agcatcatta tttgttttac gtaaaagcac
     4921 cattctcccc agagtggcgc agcttgcgca aaaacttcat gatatggttc aggaaggaca
     4981 acggatgccg tcctgtataa atctgattgg aggtccaagt tctactgcag atatagaact
     5041 gatcaaagtt gttggcgtac atggtcctgt acatgcaacc tatttgatca ttgatgattg
     5101 cgattaacga ttgctaataa aaaaatggcc ttaaggtgat atttcgtctt gtgaaaaggc
     5161 aacaatagac taaaggttag gcataattgt gtttctttat gcctccgcag caatttgtgg
     5221 aggctttttt gcttactcca aacagtttgg cttcttgaaa aagtcatatc ccaaacctat
     5281 tgacgcttat aaaaaagtaa agaatggtga gtaagcgtaa actgaccacc gtatgtagcc
     5341 atgatgccag tattggtaac gtaggtgcac ccctatctat ttttgtagaa ccggaggaaa
     5401 cagaccagat agtttaaatg agctggttat acgtaacata att
//
LOCUS       DAZGSP010000196         5350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZGSP010000196 DAZGSP010000000
VERSION     DAZGSP010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.198"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            210..1082
                     /locus_tag="ACHK74_004990"
     CDS             210..1082
                     /locus_tag="ACHK74_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP0223655.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            1410..4529
                     /gene="agn43"
                     /locus_tag="ACHK74_004991"
                     /gene_synonym="flu"
     CDS             1410..4529
                     /gene="agn43"
                     /locus_tag="ACHK74_004991"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFP0223656.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKGAGVAVALSLAA
                     VTSVPALAADTVVQAGETVNGGTLANHDNQIVFGMTNGMTISTGLEYGPDNEANTGGQ
                     WVQDGGTANKTTVTSGGLQRVNPGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQ
                     IVKEGGLADFTTVNQKGKLQVNAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSG
                     AAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAG
                     TTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGTLTGNGRVEKSGSGTLTVSNTTLT
                     QKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIP
                     DNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNA
                     DRLVIDGGRATGKTILNMVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNRLQ
                     AGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRIVAGSRSHQTGVNGE
                     NNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLESDLLRTEVAGMSLTTGVYG
                     AAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLTHTSSGLWADIVAQGTRHSMKASSD
                     NNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVRFGHGS
                     AQHVRAGFRLGCHNDMTFGEGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSSRGD
                     MRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYN
                     GQATLNVTF"
     gene            4650..>5350
                     /locus_tag="ACHK74_004992"
     CDS             4650..>5350
                     /locus_tag="ACHK74_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223657.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALPFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTSAIRQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLT"
BASE COUNT         1240 a         1448 c         1518 g         1144 t
ORIGIN      
        1 ctttacggag aaatcagcct gctgtttaac aataacggcg tattttcatg gaaggagatc
       61 acccagtaac ggataagtcc tgatctcctt catgttaata cgcaacaatc ccatcgattc
      121 aaaacaattt cagacagata ttatctccgt gaatcccccg ccacctttcc gtgcgcgggg
      181 ttttgtcttt tttcaccggg aatacatgta tgaatccgtc tgatgccatt gaggcaattg
      241 aaaaaccgct ctcctccctg ccttactcgc tttcccgtca catcctggaa catctgcgca
      301 aactcacccg tcacgaaccc gtgattggca ttatgggtaa aagcggggcc ggtaaatcct
      361 cactctgtaa tgcactgttt cagggggagg tcaccccggt cagtgatgtt cacgccggca
      421 cccgggaagt acggcgcttc cgtctgagtg gccacggtca cagcatggtt atcactgacc
      481 tgcccggggt gggcgagagc cgggacaggg atgcagagta tgaggccctg taccgtgaca
      541 ttctgcctga actggacctg gtactgtggc tgattaaagc cgatgaccgt gccctgtctg
      601 tggatgagta tttctggcga cacatcctgc accggggaca tcagcgggta ctgtttgtgg
      661 tgacgcaggc cgacaaaacg gagccctgcc atgaatggga tatggccggt attcagcctt
      721 ctcctgcaca ggcacagaat attcgcgaaa aaacggaggc ggtattccgt ctgttccggc
      781 ccgtacatcc ggttgtggcc gtatcggccc gcaccggctg ggaactggat acgctggtca
      841 gtgcgctcat gacagcgctt cccgaccatg ccgccagtcc cctgatgacc cgactgcagg
      901 acgagctgcg cacggaatct gtccgctctc aggcccgtga acagtttacc ggtgcggtgg
      961 accggatatt tgacacagcg gagagtgtct gtgttgcctc tgttgtccgt acggccctgc
     1021 gcgctgttcg tgacacggtg gtctctgttg cccgtgcggt atggaactgg attttcttct
     1081 gaacctgtcg tgactgatgc cctccctgac tctgaatctg ctcacaaaag cgctgttttc
     1141 gttactgtct ctcttgttcg tgcaatagct caacaataga ataaaacgat cgatatctat
     1201 tttatcgatc gtttatatcg atcgataagc taataataac ctttgtcagt aacatgcaca
     1261 gatacgtaca gaaagacatt cagggaacaa cagaaccaca attcagaaac tcccacagcc
     1321 ggacctccgg cactgtaacc ctttacctgc cggtatccac gtttgtgggt accggctttt
     1381 ttattcaccc tcaatctaag gaaatgctga tgaaacgaca tctgaacacc agctacagac
     1441 tggtatggaa tcacattacg ggtaccctgg tggtggcttc cgaactggca cgctcacggg
     1501 gaaaaggcgc cggtgtggcg gttgctctgt ctcttgctgc tgtcacgtca gtcccggcac
     1561 tggctgctga cacggttgta caggcgggag aaaccgtgaa tggcggaaca ctggcaaatc
     1621 atgacaacca gattgtcttc ggtatgacca acggaatgac catcagtacc gggctggagt
     1681 acgggccgga caatgaggcc aataccggcg gtcaatgggt acaggatggc ggtacagcca
     1741 acaaaacgac tgtcaccagt ggtggtcttc agagagtgaa tcccggcgga agtgtttcag
     1801 acacggttat cagtgccgga ggcggacaga gccttcaggg gcaggcagtg aacaccactc
     1861 tgaacggcgg tgagcagtgg gtacatgaag gcgggattgc aacgggtacc gtcattaatg
     1921 agaagggctg gcaggccgtc aaatccggcg caatggcaac cgacacggtt gtgaataccg
     1981 gcgcggaagg gggaccggat gcagaaaatg gtgataccgg gcagtttgtt cgcggaaatg
     2041 ccgtacgtac cactatcaat aaaaatggtc gtcagattgt ggctgctgaa ggaacagcaa
     2101 ataccactgt ggtttatgcc ggcggcgacc agacggtaca cgggcatgcg ctggatacca
     2161 cactgaatgg cggttaccag tatgtgcaca acggaggcac agcctctgac acggttgtaa
     2221 acagtgacgg ctggcagatt gtcaaggaag gtggtctggc ggatttcacc accgttaacc
     2281 agaaaggcaa actgcaggtg aacgccggtg gtacagccac gaatgtcacc ctgaagcagg
     2341 gcggcgcact ggttaccagt acggctgcaa ccgttaccgg cataaaccgc ctgggagcat
     2401 tctctgttgt ggagggtaaa gctgataatg tcgtactgga aaatggcgga cgcctagatg
     2461 tgctgaccgg acacacagcc actaataccc gcgtggatga tggcggaacg ctggatgtcc
     2521 gcaacggtgg taccgccacc accgtatcca tgggaaatgg cggtgtactg ctggccgatt
     2581 ccggtgccgc tgtcagtggt acacggagcg acggaaaggc attcagtatc ggaggcggtc
     2641 aggcggatgc cctgatgctg gaaaaaggca gttcattcac gctgaacgcc ggtgatacgg
     2701 ccacggatac cacggtaaat ggcggactgt tcaccgccag gggcggcaca ctggcgggca
     2761 ccaccacgct gaataacggc gccatactta ccctttccgg gaagacggtg aacaacgata
     2821 ccctgaccat ccgtgaaggc gatgcactcc tgcagggagg cactcttacc ggtaacggca
     2881 gggtggaaaa atcaggaagt ggcacactca ctgtcagcaa caccacactc acccagaaaa
     2941 ccgtcaacct gaatgaaggc acgctgacgc tgaacgacag taccgtcacc acggatgtca
     3001 ttgctcagcg cggtacagcc ctgaagctga ccggcagcac tgtgctgaac ggtgccattg
     3061 accccacgaa tgtcactctc gcctccggtg ccacctggaa tatccctgat aacgccacgg
     3121 tgcagtcggt ggtggatgac ctcagccatg ccggacagat tcatttcacc tccacccgca
     3181 cagggaagtt cgtaccggca accctgaaag tgaaaaacct gaacgggcag aatggcacca
     3241 tcagcctgcg tgtacgcccg gatatggcac agaacaatgc tgacagactg gtcattgacg
     3301 gcggcagggc aaccggaaaa accatcctga acatggtgaa cgccggcaac agtgcgtcgg
     3361 ggctggcgac cagcggtaag ggtattcagg tggtggaagc cattaacggt gccaccacgg
     3421 aggaaggggc ctttgtccag gggaacaggc tgcaggccgg ggcctttaac tactccctca
     3481 accgggacag tgatgagagc tggtatctgc gcagtgaaaa tgcttatcgt gcagaagtcc
     3541 ccctgtatgc ctccatgctg acacaggcaa tggactatga ccggattgtg gcaggctccc
     3601 gcagccatca gaccggtgta aatggtgaaa acaacagcgt ccgtctcagc attcagggcg
     3661 gtcatctcgg tcacgataac aatggcggta ttgcccgtgg ggccacgccg gaaagcagcg
     3721 gcagctatgg cttcgtccgt ctggagagtg acctgctcag aacagaggtc gccggtatgt
     3781 cactgacgac aggagtgtat ggtgctgcag gccattcttc cgttgatgtt aaggatgatg
     3841 acggctcccg tgccggcacg gtccgggatg atgccggcag cctgggcgga tacctgaatc
     3901 tgacacatac atcctccggc ctgtgggctg acattgtggc acagggaacc cgccacagca
     3961 tgaaagcgtc atcggacaat aacgacttcc gcgtccgggg ctggggctgg ctgggctcac
     4021 tggaaaccgg tctgcccttc agtatcactg acaacctgat gctggagcca caactgcagt
     4081 acacctggca gggactctcc ctggatgacg gccaggataa cgccggttat gtgaggttcg
     4141 ggcatggcag tgcacaacat gtgcgtgccg gcttccgtct gggctgccac aacgatatga
     4201 cctttggtga aggcacctca tcccgtgccc ccctgcgtga cagtgcaaaa cacagtgtgc
     4261 gtgaactgcc ggtgaactgg tgggtacagc cttccgttat ccgcaccttc agctcccggg
     4321 gtgatatgcg tgtggggacc tccaccgcag gcagcgggat gacgttctca ccctcacaga
     4381 atggcacttc actggatctg caggcaggac tggaagcccg tgtccgggaa aatatcaccc
     4441 tgggcgttca ggccggttat gcccacagcg tcagcggcag cagcgctgaa ggctataacg
     4501 gtcaggccac gctgaatgtg accttctgac agaaccatcg cctctttgtg gtcccggtca
     4561 tcatgatcgg gacccggacc ggcgcaacgg atattcaacg ccacattcgc tggcattaac
     4621 aataacatga tattcatcac ggagtgacta tgttacagat agtcggcgcg ctgatcctgc
     4681 tgatagcagg atttgccatt cttcgccttt tgttcagagc attaatcagc acggcttctg
     4741 cgctggcagg gctcatattg ctgtgtctgt tcggcccggc tttactggct ggctatatca
     4801 ctgaacgcat aacccggtta ttccatattc gctggctggc aggcgtattt ctgacgattg
     4861 ccggaatgat catcagcttc atgtggggac ttgatggtaa acatatagca ctggaggctc
     4921 atacctttga ctctgtaaaa tttattctga ctaccgctct cgccgctggt ctgctggctc
     4981 ttcccgtgca gataagaaca attcagcaga acgggctcac accagaagat atcagcaagg
     5041 aaattaacgg gtattactgc tgtttttata ctgccttttt ccttatggcg tgttctgcat
     5101 acgcaccatt gatcgcattg ccgttcgata tttcaccctc actgatgtgg tggggcgggt
     5161 tgttgtactg gctggctgca ttagtgacgc tgctatgggc ggccagccag atccaggcgc
     5221 tgaaaaaact gaccagtgcc atccgccaga cactggaaga acaaccggtg ctcaacagta
     5281 aatcgtggct gagcagtttg caaaacgatt acagccttcc tgaaacgttg acggagcgca
     5341 tctggctcac
//
LOCUS       DAZGSP010000197         5319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZGSP010000197 DAZGSP010000000
VERSION     DAZGSP010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.199"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            167..682
                     /locus_tag="ACHK74_004993"
     CDS             167..682
                     /locus_tag="ACHK74_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP0223658.1"
                     /translation="MRFICDNSYFLEGVKEYINPGKFIISGMQTDNVVIISSSRISEI
                     VGFLYKNNISDMNTLYFSSPEALRFLWGVVDSPVYDIRHLTERCSVNDISHLYRAFMS
                     VEKLTLSGRQVNVLMMLLYGSNGTEGARYLGMSEKTFSTHKQNLIKRLRVHNEVELLY
                     KGMLLVRKGRL"
     gene            757..945
                     /locus_tag="ACHK74_004994"
     CDS             757..945
                     /locus_tag="ACHK74_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFP0223659.1"
                     /translation="MRLLIAFLLPWLTFFTIGRPLTGIFCLVLQITLLGWIPATIWAV
                     YALSQYKTEQKIREATGK"
     gene            1059..1937
                     /gene="mscS"
                     /locus_tag="ACHK74_004995"
     CDS             1059..1937
                     /gene="mscS"
                     /locus_tag="ACHK74_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFP0223660.1"
                     /translation="MDASGEAVSWIVRNQDILLGYAVNLVAAVVIIIIGGIAARMISG
                     VLNNLMLGRNIDATVAQFLSVLIRHAIIAFTLIAALGRVGVQTASVIAVLGAAGLAVG
                     LALQGSLSNLAAGILLVMFRPFRAGEYADLGGVAGTVQNVHIFSTTMRTLDGKIVVIP
                     NGKIIAGEIVNFSREPERRNEFIISVSYDADIDRVKQVLTDIVMSEERVLKNREITVR
                     LNELGESSMNFVVRVWSRRDDLQSVYWDVLERIKREFDSEGISFPYPQMDIHLVRSGK
                     QAESVTHNSRSVSSRQ"
     gene            2063..3283
                     /locus_tag="ACHK74_004996"
     CDS             2063..3283
                     /locus_tag="ACHK74_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFP0223661.1"
                     /translation="MPKKIMTGIDVFDAAMERIEWVFDTFSSVCLSFSGGKDSTVLFH
                     LMANIARRKKRRFSVLFVDWEAQYQCTIEHVTRLREMYDDITDTFFWVALPLTTVNGV
                     SQVQPEWVCWEDGAEWVRQPPEDAITRASFFPFYQNAMTFEEFIPAFNHWFAREKGMV
                     AAILTGVRADESLNRFMGLVSGRKLRYAEDKPWTTASAEGFYYTMYPLYDWKTRDVWI
                     YHARTRAAYNSLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPDTWGRMCARV
                     SGAVGGALYANESGAYFALRKQITKPVHHTWQSYAMFLLESMPEKTAEHYKNKIAVYL
                     RWWQSHGWSDGIPDEQDNDQGCKDIPSWRRICKTLLKNDFWCRMLAFSPNKPRHYERY
                     LARMREKRKEWGIL"
     gene            3512..5257
                     /locus_tag="ACHK74_004997"
     CDS             3512..5257
                     /locus_tag="ACHK74_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP0223662.1"
                     /translation="MTAGEKRVASRLEAFMSNECLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVHTLHQVNHEQVILETNGILKSEINPLVQVRRYACDTVNALPADSRL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKALAGNDENAVERIFPSAQTICQDDMTQS
                     VLPDIFRQKIAGMFTMGFRTRVTPQVRDILRAHLFPEMTVRQHSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIESRGL
                     KSRVTVFHFHSWCASMIKRHGIQVTAGGKDYPEKCFSALEDAVNSGTITDTGYDAVLV
                     DEGHDFESRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNTKRILHFAYAFSREYFEKHQNREIPFVQPQAGGEEGTEPEILRCASESDE
                     AVQVVGWLEKRYTLCGHWSDMAVLCPAEFSVKHLKEVMTQRGIPYATCFDSEGKKKYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPALYVAFTRATRELLVT
                     CYRENSISRHLEDFV"
BASE COUNT         1334 a         1005 c         1432 g         1548 t
ORIGIN      
        1 aggtgtcagt gttaaatatc tctgcagcac cgcagaccgg taaaaagccg gtctgatgaa
       61 ggctgttgcg acgggaattt ctgtgcatgc tgtgtcacgg gcatcccggt gctccggata
      121 attaatgtta tgtggtcagg cgtgataaat ttcatgtgga acgggtatgc gttttatttg
      181 tgataacagt tattttctgg agggagtgaa ggagtatatt aatcccggta aattcattat
      241 cagcggcatg cagacagata atgtggtgat aatatcttct tcccggatat ctgaaattgt
      301 cggctttctg tataagaata atataagtga tatgaacacc ctttattttt cttctccgga
      361 agcgctccgt tttctctggg gagtggttga cagtccggtt tatgatatca gacatcttac
      421 tgagcgatgt tcagtaaacg atatcagtca tctgtaccgc gcattcatgt cggtggagaa
      481 gctgaccctg tcaggccggc aggtaaatgt actgatgatg ctgctttatg gcagtaacgg
      541 aacagagggg gcacgatatc ttggcatgtc agagaaaaca ttctccactc ataagcagaa
      601 tctgataaag agattacggg tacataatga agttgagttg ttgtacaagg ggatgcttct
      661 ggtcagaaaa gggaggttgt gaagcaggac agcttatttc cggtaatgtt aaacgcagcc
      721 tctgattcgt taatttcata atcataagga gtttttatgc gtctgttgat tgcttttctt
      781 cttccctggc tgactttttt caccatcggc cggcctctga cggggatttt ttgtctggtg
      841 ttacagataa cgctgctggg ctggataccg gcaacaatat gggccgtgta tgcgctttcg
      901 cagtataaga cggaacagaa aatccgcgaa gccacgggga aatgatgact gcagaagagc
      961 gggttgtcat agctgtgtat atgatgcggg ataaaggccc ggagattcct gaaaataaaa
     1021 tggttcgtgg aatataaaac agggaaaaac cggatgtgat ggacgcttct ggcgaggcgg
     1081 tttcatggat tgtccgtaat caggatatat tactcggtta cgccgtgaat cttgtggcag
     1141 cagtggttat tattattatc gggggtatcg ctgcacggat gatttctggt gtgctgaata
     1201 atctgatgct gggcaggaat attgatgcca cagtggcgca gttcctttcc gtgctgatac
     1261 gtcatgccat tatagccttt acgctgattg ctgccctggg acgtgtgggg gttcagacag
     1321 cttccgtaat tgcagtcctg ggggctgcag gtcttgctgt tggacttgcc ctgcaggggt
     1381 cattgtcaaa ccttgcagct ggtattcttc tcgtgatgtt ccgtcctttc agggctggtg
     1441 aatatgctga cctgggtggt gttgccggga ctgtgcagaa tgtacatata ttctcaacca
     1501 cgatgcgtac actggatggt aaaattgttg tcattccgaa cgggaagatt attgccggag
     1561 agattgttaa tttctccagg gagcctgaac gacgaaatga gtttatcatc agtgtttcat
     1621 atgatgcaga tattgaccgt gtgaaacaag tcctgacaga tattgtgatg tcagaagaga
     1681 gggttctgaa gaacagggaa atcactgttc gcctgaatga gcttggggag tcatccatga
     1741 attttgtagt acgggtatgg agcaggcgtg atgaccttca gtctgtatac tgggatgtac
     1801 tggagagaat taagcgggag tttgattcag aagggataag ttttccatat ccacaaatgg
     1861 atatccatct tgttcgctcc gggaagcagg cagagtcggt aacccataac agtcgctcag
     1921 tcagttccag acagtaaaat gaaaagcgca ttttatttgt gaaagatgaa cagcctgaat
     1981 ctgtcaggct gttttcattg tgtttttatg gtcatttttg gtatatgtac tgtatatgat
     2041 gtcatctgta taaggatata aaatgccaaa gaaaataatg acagggattg atgtttttga
     2101 cgctgccatg gagcgtattg aatgggtgtt tgatacgttt tcatcagttt gtctttcttt
     2161 ttccggcgga aaagactcca ctgtcctgtt tcacctgatg gcgaatattg cccgccgaaa
     2221 aaaacgccgt ttttctgtac tgtttgttga ctgggaggct cagtatcagt gtaccattga
     2281 gcatgtcacc aggttgaggg aaatgtacga tgatatcaca gatacttttt tctgggtggc
     2341 gctgcctctg acgaccgtta atggagtgtc tcaggtacag cctgagtggg tatgttggga
     2401 ggacggggct gaatgggttc gccagccacc agaagatgcc ataaccagag cgtctttttt
     2461 tcctttttat cagaatgcga tgacatttga agagtttatt ccggcattta atcattggtt
     2521 tgcccgggaa aaagggatgg ttgctgcgat tcttactggt gttcgcgctg atgagtcact
     2581 gaatcgtttt atgggactgg tatccggacg taagctccgt tatgcggagg ataaaccctg
     2641 gaccacggca tcggctgagg gattttacta caccatgtat cctttgtacg actggaaaac
     2701 aagagatgta tggatttacc atgcgagaac acgtgctgcc tataactctc tttatgacct
     2761 gatgtacagg gctggagttc ctcttcgcaa tatgcgggtg tgtgagcctt ttggaccgga
     2821 gcaacgtaag gggttgtggc tttatcatgt actggagccg gatacatggg gcagaatgtg
     2881 tgctcgcgta tcaggtgctg tagggggagc gctctatgca aatgaaagtg gtgcttattt
     2941 tgctttgagg aaacagataa caaaaccagt tcaccataca tggcagagtt atgcaatgtt
     3001 tctgctggag tcaatgcctg agaaaacggc ggagcattac aagaataaga ttgctgttta
     3061 tcttcggtgg tggcagtctc atggatggtc ggatggcatc cctgatgagc aggataatga
     3121 ccagggatgt aaagatattc cctcctggcg tcgcatctgt aagacgttac tgaaaaatga
     3181 tttctggtgt cggatgctgg cgtttagtcc gaataagccc cgacattatg agcgctacct
     3241 ggccagaatg cgtgaaaaaa ggaaagagtg gggaatattg taaattatat ttaaacatcc
     3301 gaaatatgac ggttaatcca ggatagtagc taaaaaataa gatttcttgc tgatttatcc
     3361 ctgtttgata tgtaaaatct gaggggctta taagcaacgg atatactagt gctacaatat
     3421 atgataagtt ttatatatta aatgttcatc atctgtcagg ggctgtttta tggctaaact
     3481 gataaatata tcaggtgtta ccgaggagcg gatgactgcc ggggaaaagc gggttgcatc
     3541 ccgtcttgag gcatttatga gtaatgagtg tctggtctgg tacgatattc cggtgggacg
     3601 gaaaaaccga catcctgatt ttgtcattat tgacccggag aatggtctgg tatttcttga
     3661 agtcaaggac tggaccgttc acacgttgca tcaggtaaat cacgaacagg tcattcttga
     3721 aactaacggt attcttaaat cagaaatcaa tccgctggta caggttcgtc gttatgcctg
     3781 tgataccgta aatgctttac ctgcagactc acgtttgcga cagaatgatg gtcagtataa
     3841 ggggcgtctg aatctggcct gggcgtatgg tgttgtgttt acccgtatta cccgccagca
     3901 actgaaggct ctggctggga atgatgaaaa tgctgtggaa aggattttcc cttcagcgca
     3961 gacaatctgt caggatgata tgactcagtc tgttttacct gatatattcc gccaaaaaat
     4021 tgccgggatg tttacgatgg gattcagaac ccgggtgaca ccacaggtta gagatattct
     4081 gcgtgctcat ctgtttcctg aaatgaccgt cagacagcac tcacaaatca aaataatgga
     4141 tattcagcag gagattctgg ccagaaatat tggtgatggg catcgtgtta ttcatggggt
     4201 agccggctct ggaaaaacaa tgattctttt gttccgttgt ctttacttgg ctgaaactac
     4261 ccctggtaag attctggtgc tatgttacaa cattactctg gccagctacc tgcgggaatg
     4321 tattgagtcc aggggactga agtcacgggt tacggtattc cattttcata gttggtgcgc
     4381 ctcaatgata aagcgccatg gaattcaggt gactgctgga ggaaaggatt acccggagaa
     4441 atgcttcagt gcgctggaag atgccgtgaa cagcggaaca attactgata ctggatacga
     4501 tgccgtactt gtggatgagg ggcatgattt tgagagtcgc tggctggctc tgatagcccg
     4561 cctgtttgat aacgcatccc gttcactgct cctgatgtac gatgacgcac agtccattta
     4621 cagacgggaa cgggctctga acttttcgct tgccagtgtg gggattcagg cgcagggacg
     4681 tacttccgtc ctgccggtaa attaccgtaa tacgaaacgc attctgcact ttgcttatgc
     4741 attctcccgt gagtattttg agaagcatca gaaccgtgaa ataccgtttg ttcagccaca
     4801 ggcgggcggt gaagagggca cagagccgga aattctgcgt tgtgcttctg agagtgatga
     4861 ggcagtgcag gttgtgggct ggctggagaa acgctataca ctgtgcggtc actggagtga
     4921 tatggctgtt ttgtgtcctg cagagttttc agtgaaacat ctgaaagagg tcatgacaca
     4981 acgcgggata ccttatgcca cctgctttga cagtgaaggt aagaagaaat attcgcgacg
     5041 caaggatgtt gtgcatttac tgacctacca gagcagtaag ggactggagt ttccgtatgt
     5101 tgcggttatc aatgcttctt tcgttcactc aggtgctgcg gatgagtctg aagttattcc
     5161 ggcgctttat gttgcgttca cccgggctac acgggaatta ctggtgacct gttatcgtga
     5221 aaacagtatc agtcgtcatc ttgaggattt tgtataagtt atgttttatt attgctgata
     5281 tgtaatcatt cgttttttga taatctaaga tgggtattt
//
LOCUS       DAZGSP010000198         5069 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZGSP010000198 DAZGSP010000000
VERSION     DAZGSP010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.200"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..179)
                     /locus_tag="ACHK74_004998"
     CDS             complement(<1..179)
                     /locus_tag="ACHK74_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223663.1"
                     /translation="MQKLENRCELLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGTIISVAP"
     gene            complement(265..471)
                     /locus_tag="ACHK74_004999"
     CDS             complement(265..471)
                     /locus_tag="ACHK74_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0223664.1"
                     /translation="MNTPVSLMADQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVETWLQARIAQSRP"
     gene            1328..1552
                     /gene="hha"
                     /locus_tag="ACHK74_005000"
     CDS             1328..1552
                     /gene="hha"
                     /locus_tag="ACHK74_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFP0223665.1"
                     /translation="MQHKVTRDEWKLKPEQFRRCSSRETLEKVITRTRYKLTPAELEA
                     FNSAVDHRLAELTMNKLYDRVPASVWKYVT"
     gene            complement(1884..2081)
                     /locus_tag="ACHK74_005001"
     CDS             complement(1884..2081)
                     /locus_tag="ACHK74_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223666.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGAVDPFGYYRKTDRLLCLL
                     TLLLVLILVTVSGVSVFVLLQ"
     gene            2280..2849
                     /locus_tag="ACHK74_005002"
     CDS             2280..2849
                     /locus_tag="ACHK74_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFP0223667.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGRYDTLAHFPDKPAVWLERDDETGFQ
                     QVLERSGYLAKEHTKARGTGERNFGCSRS"
     gene            3110..3493
                     /locus_tag="ACHK74_005003"
     CDS             3110..3493
                     /locus_tag="ACHK74_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP0223668.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHGHECPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGRFSEEGGAE"
     gene            3490..3903
                     /locus_tag="ACHK74_005004"
     CDS             3490..3903
                     /locus_tag="ACHK74_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP0223669.1"
                     /translation="MNGLKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCERLF
                     HGKKFCEACGTGIYCRAVCTKSVYRYQPDLLRNPE"
     gene            3909..4088
                     /locus_tag="ACHK74_005005"
     CDS             3909..4088
                     /locus_tag="ACHK74_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223670.1"
                     /translation="MQHNCLITPDTPLPDPDVVRRFVLRTVVQWLCLLMLLFMRHSDR
                     GDIDKHHLRANSYTE"
     gene            4209..4832
                     /locus_tag="ACHK74_005006"
     CDS             4209..4832
                     /locus_tag="ACHK74_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0223671.1"
                     /translation="MRTRIDYLADKYSFTELNELPRLHQQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVIGNENTSSPYQQIAREVKAPRERLKHALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKSGMPVVTTTAEVHDNLTGTTRKVPAYHL"
     gene            4921..>5069
                     /locus_tag="ACHK74_005007"
     CDS             4921..>5069
                     /locus_tag="ACHK74_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223672.1"
                     /translation="MARSDYDIVNLSLEHELNEWLAEIGYAGLVDNRNRLAEVVTRKL
                     QDSFY"
BASE COUNT         1238 a         1230 c         1412 g         1189 t
ORIGIN      
        1 ggtgccactg agatgatagt gcctttttct ggcagaaaaa agtgtgccag ggtcaggcgg
       61 tgataaccat gaagatgcgg agaccccatt ccatgagcga cgataagcct cgtcacgctt
      121 gtcatccatt tctgatgctg gatgagcagc agctcacacc ggttttcaag tttttgcata
      181 atattctctc ctttagcgcg cagcaaaatg cctccggggg ccggatcatg cctttgctgc
      241 ggttgaacgg ataacggtca ggaattacgg acgggactgt gcaatacgcg cctgcagcca
      301 ggtttccact tcacttttca gccagcggga actgcggccc agtttgatgg gggcaggaaa
      361 ggcaccatcc ttgatgagct tgtaaaacca tttatcggtc aggccggtca gttgagtgat
      421 aaatgccatg tcgaccatct ggtcagccat cagtgaaact ggggtattca tgaaaatgtt
      481 cctccggatg ttgttaatct caaccggcgt tggtgatgaa aacaacgcac caccttcacc
      541 ggcataacaa ccggggagaa acgtaaaaaa taatcgggaa tgtgaccgtc aggagataaa
      601 cggggggatt gcggtggctg agccgtaccg gtatgtcggg tgattgttgc gaaacgatgc
      661 ttccgcacgc gcacgggagg actttcgtga gacaagggga aaactggcgt tctgataatt
      721 gcagagttgt ttttgaaggc ggttgtggtg catcagcctg tgacctgtgg gcaggcgaca
      781 ggtatcactg gtgggataag actgtctgat tggtattata aatatataag agagctcaaa
      841 cacatgagtt catatatgtg aaataaatct gtgagtgtga tcagcagacc agtctaccca
      901 gtagcaacag attgcgttgt actcacaggg ttatccctga taactggcgt ataacctgcg
      961 tacataaaac gtacctgcaa tgcacctgaa cagagtgcat taagcatccg cctgagcggt
     1021 aaacgtatcc gatctggtga gtgacagtct gagagcattc tcagtaatcg cccccttgta
     1081 tcctggaaat tggttaagag cgattagatt tggcaaggtg tatgcatcca gcagcttaac
     1141 acctcagcta actggctggt taccttcagg ttgtcgcaat ggcatcggac atccggtgtc
     1201 ggataactaa acgtagcgtt aaggcgatat tgctgagaga ccggttaagt ggtggcacgg
     1261 gagtaatgag tttggggagg gtaccagaat gccagtcagg tgcggtatgc tggcagtatc
     1321 acgttgattg cagcataaag taacgagaga cgaatggaag ttaaaaccag agcagttccg
     1381 tcgttgttca tcccgggaga cgctggaaaa agtgattacc cgcacacgtt ataaacttac
     1441 ccctgcggag ctggaagcct ttaactctgc ggtcgatcac cggctggcag aactgacaat
     1501 gaacaaactt tacgatcgcg tgccggcttc cgtctggaaa tatgtcacct gaatcttgaa
     1561 tccgcgctac gatgactctg gtgttatctg tgcaccggaa gatcgggaac attaagcagt
     1621 ataacggcaa caaagaattc gaaatcaaaa gggaatggtg tacagagggg caggcgttca
     1681 ggcattaccg gcctgtatct tctgaagggg actcgtattt gtcggggctg agtattccgc
     1741 tcgattcgat cacggattgg cagaaacctg actgtgattg attcaggtac cattttgcag
     1801 ggatccggtg cgttactgtc tggcacaaca aacgcagcgg gtcgctctgc cccgccgatt
     1861 cctgataatt cctcccctgt aacctactgc aacagtacaa atacactgac gcctgaaacg
     1921 gtaaccagaa tcagcaccag cagtaacgtc agtaaacaga gcagacgatc ggtcttccgg
     1981 taataaccga aggggtcgac agcgccacaa tacgggcaac acactgtatt atgagtaatt
     2041 gcgtttccac agtccctgca cttctttttg cgtatcacga tcgctccctc tccacgcctt
     2101 atccgtaacc tgttttttac attaaaaaaa caacgccggg aattcaatcg tcagtccgga
     2161 gacggccgtt cgggttatca cagagtgcct gagacagtgt cctgccggag gtcacctcag
     2221 gggctctgcg cattttttac gacgtggcta ttccgtatga accatacgga gatttaacca
     2281 tgacctacaa atacaacccc ttctggcagc aacgtattcg tgagacggtg cggcacgcac
     2341 tggatgttca tccccgcctg acggcacttc gggttgatct gcgtctcccg gatgtgccgg
     2401 cagcaacgga cgcggctgtg atatcccgct tcatcaatgc cctgaaagcc cgaatcgacg
     2461 cttatcagaa acgtaagcat cgggaaggta aacgcgtgca ttcctcgacc ctgcattacg
     2521 cctgggcccg ggagttcggg gagcttaaag gcaaaaaaca ctatcacctg ctgctgctgg
     2581 tcaaccggga tacctggtgt cgtgccggcg attaccgcgc tccggggtca ctggccggga
     2641 tgattaaaca ggcctggtgc agtgccctgg gagtggatgc cggacgttat gacacgctgg
     2701 cacattttcc tgacaagcca gcggtgtggc tggaacgcga tgatgaaacc ggctttcagc
     2761 aggtgctgga acgttctggc tatctggcga aggagcatac caaagctcgc ggcaccggtg
     2821 agcgcaattt tggctgtagc cggagttgaa cacgacaggc gcaccaatac ccggcaaacc
     2881 aaaagtatgg gttgttgtct ggcggcaacg atgtatccac tcgcccactg actgtttttt
     2941 acgtcagtgg gccattgtgt gacaaccaca gtgctgtaac agcaaccagc ctgctttacc
     3001 caaactccag tccagatttt aactgaaccg ccattcacac cctgaccacg ctgcccgtac
     3061 cgggacggct gttgcctgcg tgtcgtctgg cggctgagga acatatacta tgtacgcaaa
     3121 atcctttctc gctcttgatg gcaacggacg tctgacgggg gcccgtaccg cacagactgc
     3181 gccttacgac cgctacacct gccacctttg cggcagtgca ctcagatacc acccgcaata
     3241 cgacactgaa cgtccctggt ttgaacacac tgacgacggg ctgacggagc acggtcatga
     3301 gtgcccttat gtcagaccgg agcgcaggga gatacaactg attaaacgtc tgcagcaatt
     3361 cgtaccggat gccttacccg tagtacgtaa agccagctgg cactgcaggc aatgtcacca
     3421 cgattattat ggggagcggt actgcacaca ctgccagacc gggcgcttca gtgaggaggg
     3481 gggagcagaa tgaacggtct aaaaacgggg ctggggatca cgccgggtga gcacattatc
     3541 agtgccgact ctgcgctcag cagaaatatc cggcagtgtt tctgcctttc ctgtcatggc
     3601 cggttgattc tgcagacaga tgctcagggg gcgtggtttg agcacgattt acatgccctg
     3661 agcgagcaac agaaggcgga ctgtgtggtg ctgaatccgg agaagtccca cccttacatt
     3721 gaggatatgg cgatgttttt atcgccatta ccggtcgtgc tggagtggca ttgtgtgatg
     3781 tgtgagaggc tttttcatgg gaagaagttc tgtgaggcct gtggtacggg gatttattgc
     3841 agagcggtat gtacaaagtc tgtttacagg taccagccag atttgctccg gaacccggag
     3901 tgaggaggat gcaacataat tgtttaatca cgccagacac gcccctgccg gatccggatg
     3961 tggtccggcg ttttgttctg cggacggtgg ttcaatggct gtgtctgctg atgctcctct
     4021 ttatgcgaca ttcagacagg ggagacatcg ataaacatca tctgcgggca aatagttaca
     4081 cagaatgatc attgctaccg gttatggcaa aaattggtga tgaccaggtg aaccatcggt
     4141 gctttcgttg attcttatgg actgataaag ttttgtcagc tgaatcttta tacggaattc
     4201 ttttcagtat gcgtacacga attgactatc ttgcggataa atacagtttt actgaactga
     4261 atgaacttcc acgccttcac cagcagtggc aggatgttct ggaggagtgc cggcagacag
     4321 aggccgggcc ggaagaacgg ctgcgtattg cactgctgaa tgtggattac gtcaccagtt
     4381 ttgaactgcc ttttcgcttg ttgcttaccc gtacaccaca actgattgcc gcgctgcgtg
     4441 aagaatgggg catcagccag aaaaatgtgg tgttcaatga taaacgattt ggctgcgtgt
     4501 acagcctgaa ggccagcctt tccggtgtgc cggatacata ccggtatcat ctgtctcatc
     4561 gtattcgccg ggtgattggg aatgaaaaca catcatcgcc ataccagcag attgcccggg
     4621 aagtgaaagc gccccgtgaa cggctgaagc atgcgctgga ggcggggttg ctggtgactg
     4681 cactggacgg cctgttctgg ttcggcagtc agcgtattgc ggctgatgtc ctgaggctga
     4741 gaaagagcgg aatgccggtg gtgacaacga ccgcggaggt gcatgataat ctgacgggaa
     4801 caacccgcaa agtaccggcc taccatctct gattcgacca tcagggggaa tattgctctg
     4861 gcagcagcag tacgacattc tttatccccg agcctgaaaa catgtgctaa ggaaacggga
     4921 atggcaaggt ccgattacga tattgtcaat ctgtctctgg aacatgaact gaatgagtgg
     4981 ctggcagaga taggttatgc tggactggtg gataaccgga accgcctggc agaggtggtt
     5041 acccggaaat tgcaggacag cttttatat
//
LOCUS       DAZGSP010000199         5044 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZGSP010000199 DAZGSP010000000
VERSION     DAZGSP010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.201"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(170..325)
                     /locus_tag="ACHK74_005008"
     CDS             complement(170..325)
                     /locus_tag="ACHK74_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0223673.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYEFEK"
     gene            complement(525..950)
                     /locus_tag="ACHK74_005009"
     CDS             complement(525..950)
                     /locus_tag="ACHK74_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP0223674.1"
                     /translation="MRDIQQVLSRWGAWVVNNHESVAWSGVAAGFKGVIPSKVKSRPQ
                     CTDDDALIISNCMAQLNVNNSDLHDFLYDYYVFGMTLMGLGRKHERSDCWAGRVLQKA
                     EGVIEGMLIIQGIKLEMDGYVERERSGAQASQFSGRTGN"
     gene            complement(1029..1892)
                     /locus_tag="ACHK74_005010"
     CDS             complement(1029..1892)
                     /locus_tag="ACHK74_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0223675.1"
                     /translation="MRTDNQVHKALFTIPTAAYSAVPANIKPLPEQRRITGHKQTDAY
                     LWILEVIHLNEAVHLDAAEAALEKLKITPEEASERYGRYLQEINVDPFQIAFATIGMD
                     NPAQAIRNARENIKKAASVRATFGSYEAALDDVEAERIIRTSPKFIDDYYWGWTAAEK
                     KAGSIDGVRSNEIDDQRRAYVDGYRDVLPEPHTLSDVVREFIYWDWLYEMRQTAGRET
                     GDKYGFTGEHHESVYDRQFWLENLLGKIKPVTRDEAVEVCRWFLASGKDEYMEDNGSA
                     VILNLVGECEQ"
     gene            complement(1892..2860)
                     /locus_tag="ACHK74_005011"
     CDS             complement(1892..2860)
                     /locus_tag="ACHK74_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFP0223676.1"
                     /translation="MKTAEAAKGHWPEILKHYGLPPVTGKKHFKGECPVCGARGKFRI
                     DDRDGTGTWICVCGSGDGMKLIALTQKRAFHEICAEIDRITGNEYRRDKPPVICTSES
                     LRSRVQRRFSALTALQGTSGAEYLRSRGIFSLPVEGVRFNSQQNYNGRMFQSLYALAT
                     DDKGELCYLHQTLLDGAKKADIGISARRLKSLQDDNYLDHARSVAIRMFPVASTLGIA
                     EGIETALSAHQIYKVNTWATINSGFMKKFRVPAGVTHLIIFADRDEYSATGMAAACEC
                     AHANLQARNDIQRVSVRWPDHDDFNNMLMNGDQVRELVFYRKKAVA"
     gene            complement(2862..4520)
                     /locus_tag="ACHK74_005012"
     CDS             complement(2862..4520)
                     /locus_tag="ACHK74_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP0223677.1"
                     /translation="MLNIKPNFAQERALNMLRHDWKTYQSFMMYMPTGSGKTGLAAFI
                     TAGLVSRGMRVLFVVPYTILINQTAQRFVQYGLSGDEISFIWRDHPNNDPSRLIQIAS
                     ADTLIRRDFPQNIDLLIIDEAHLRKRRILTEIERLVAEGNVKVIGLSGTPFSSFLGRY
                     YQRLLKPTTIGELIQRGELSNYEFYAPTKPDLEGVKTKVSMEYGRDYDESQLAEIMCG
                     ADLVGDIVDNWLCNGRDLPTVAFCVNKTHASFVTMQFNKAGINAEVMVAETPHDERQL
                     MISRFETGATKIIVSVGVLVAGFDSDVRCIIYARPTKSEIRWLQAIGRGLRTAPGKES
                     CLIFDHSGTVHRLGFPENIEYDELPGKNDGMQVSSSRGNEIREEKLPKECPGCHFMKP
                     AGVYVCPKCGFKPLAGEDVNTDTRRNIRKLSKDEKIYTKSDKQAWWSQIKFYQRQRAS
                     LGRPVSDGWCAHTFREKFGEWPDGLSSFPMEITPEVSNYIRHKFIRFARGRSRAEKMT
                     EKSPEALSLSLLHGVRSGVPEGSESWQIMQAKQQLQKNRNSLGQ"
     gene            complement(4554..4922)
                     /locus_tag="ACHK74_005013"
     CDS             complement(4554..4922)
                     /locus_tag="ACHK74_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223678.1"
                     /translation="MSAELKLVLINLLEEEFGHGMDKVTFDYVLQKKIKSLGCELQRC
                     FTVRLKGDRKGFIDLLVISPDGQRCAVEVDNRSPRQRSLMKIRALPEGISGFVFLRDG
                     KHPQRYIADGVDVIRATRFK"
BASE COUNT         1193 a         1403 c         1104 g         1344 t
ORIGIN      
        1 catcctttta tgaaaatcaa cgttttttga gcactcgcac acaaaaaatt accgcatggg
       61 ttgcaacgac aacctgcatt ttgcggagca tatgacaaat aaagtacggg tgcgttgagg
      121 atgcctgaca catcagaggt ggcgggagat tactcccccg ccaggtctct tacttctcaa
      181 attcgtagtc tacgaagaca gcgacctccg tctggccggt tctgatccgt acctcacaga
      241 ggtctttcct cgttaccagt gccgtcacaa tgacggttaa acagatgacg atcagggcga
      301 ttaacatcgc cttttgctgc ttcatagcct gcttctcctt gccttccggc acgtaagagg
      361 ctaacctgca tgtgttcagc atgaaattgg cctcagatta atgttaagcg tcttgcagga
      421 cgcgtaatgt taactggggc ttttctccgt ctgccttacg gcggcgtgcc cgaggcagac
      481 accctcaagc acccgcagtc attctacagt aaaaccccgc gctttcaatt tcccgtacgt
      541 ccggaaaact gactggcctg tgcccctgat cgttcacgct caacgtatcc gtccatttcc
      601 agttttattc cctgaataat caacatcccc tcaataacac cttctgcttt ttgcagtacc
      661 cgcccggccc agcaatctga gcgctcatgc ttacggccca gtcccataag cgtcatcccg
      721 aacacataat aatcatacag aaaatcatgc aaatcgctgt tgttgacgtt cagttgcgcc
      781 atgcaattgc tgataatcag cgcatcatca tcggtacact gaggccgtga tttaaccttt
      841 gatgggatca cgcccttaaa tccggcagcg acgccggacc atgccacact ctcatgatta
      901 tttaccaccc atgcacccca tcgggacaga acctgttgga tatcacgcat tattctgtac
      961 tccgctgtaa ccatactttt tctgatttga atgctcaggg ggaaaatgtc tactggatat
     1021 ctccggcttc attgctcaca ctccccaacc agattgagaa tgaccgctga accattgtct
     1081 tccatgtact catcttttcc gcttgccaga aaccagcgac acacttcaac cgcttcatca
     1141 cgcgtcacag gctttatttt tcccagcaaa ttttcgagcc agaactgccg atcatatact
     1201 gactcatgat gttcacctgt gaaaccgtat ttgtcccctg tttctctccc cgctgtctgg
     1261 cgcatctcat aaagccagtc ccagtaaata aactcacgaa caacgtctga caatgtatga
     1321 ggctctggca gtacatcacg atagccatca acatatgcac gacgctgatc atcaatttca
     1381 tttgagcgaa caccgtcaat acttccggct tttttctctg ccgcagtcca gccccagtag
     1441 taatcgtcga taaatttcgg ggaggtgcga attatccgct cagcctccac atcgtcgagt
     1501 gctgcctcat agctgccaaa tgtggccctg actgatgcgg cttttttgat attctcacgg
     1561 gcattcctga tcgcctgcgc cgggttatcc atgcctatgg ttgcgaaagc aatctggaaa
     1621 ggatcaacat taatctcctg caaataacgt ccataccgtt cactcgcttc ttccggcgtt
     1681 attttgagtt tctccagtgc tgcttcggct gcgtccagat gtacggcctc gttcaggtga
     1741 ataacctcca gaatccagag ataagcatca gtctgtttat gtccggtgat cctcctttgt
     1801 tctggcagag gtttgatatt tgccggaacg gcgctgtacg ctgccgtcgg gatagtgaat
     1861 aaagctttat gtacctgatt gtccgtacgc atcacgcaac cgcctttttt ctgtaaaaaa
     1921 ccagttcccg aacctgatcg ccgttcatga gcatattgtt gaaatcatca tgatcaggcc
     1981 accggacact tacccgctga atgtcatttc gggcctggag atttgcatgt gcgcattcac
     2041 aggccgcagc cattccggta gcactgtatt catcgcgatc ggcaaaaata atcagatggg
     2101 tgactcccgc cggtacgcgg aactttttca taaaaccact gttaatggtt gcccaggtat
     2161 ttactttgta aatctgatgt gcggacaacg cggtttcgat accttcagct atacccagtg
     2221 tactggctac cggaaacatc ctgatagcaa ctgaacgggc atggtccaga tagttatcgt
     2281 cctgtaaaga tttgagcctt ctggcgctga taccaatgtc tgctttttta gctccatcaa
     2341 gcaatgtctg atgcaggtaa cacagttctc ccttgtcatc ggtcgccagg gcataaagag
     2401 actggaacat gcgcccgtta taattttgct ggctgttgaa gcgaactccc tcaaccggaa
     2461 gactgaatat gccacgggaa cgaagatatt cagcaccaga tgttccctgt agcgccgtga
     2521 gcgctgaaaa tcgacgctga acgcgcgatc tcaggctttc cgacgtacag atcacaggcg
     2581 gtttatctct ccggtattca ttccccgtga tgcggtctat ttcagcgcaa atttcatgaa
     2641 acgctctttt ttgtgtcaga gcgatcagtt tcatgccgtc accgctacca caaacacaga
     2701 tccacgttcc ggtgccatca cggtcatcaa tacggaactt acctctggca ccacaaaccg
     2761 gacattcccc tttgaagtgc tttttacccg tgactggcgg cagtccgtaa tgttttaaaa
     2821 tttcaggcca gtggcctttc gctgcttctg ctgttttcat cttactgacc cagactgttt
     2881 ctgtttttct gaagttgttg ctttgcctgc atgatctgcc acgactcact gccttccggc
     2941 accccagacc ggacaccatg aagcagggac agagaaagcg cttcggggga tttttccgtc
     3001 atcttctctg cccggctacg ccctctggca aaccggatga atttgtgtct gatgtaatta
     3061 ctgacctccg gggtgatttc catcggaaaa ctgctcagcc cgtcaggcca ttcgccgaac
     3121 ttctcccgga aagtgtgagc gcaccatcca tcgctgaccg gtcgccccag agaggcacgc
     3181 tgacgctgat agaatttgat ctgactccac caggcctgtt tgtcgctttt ggtgtaaatt
     3241 ttttcgtcct tgctgagttt cctgatattg cggcgggtat ctgtgttcac atcctcacca
     3301 gccagcggtt taaatccgca tttcggacag acataaacac ctgccggttt catgaaatga
     3361 caccccggac attctttcgg cagtttttct tcccgaattt cgttaccacg tgatgatgac
     3421 acctgcatcc cgtcgttttt gccgggcagt tcgtcatact caatattttc aggaaatccc
     3481 aggcgatgaa ccgtaccgga atggtcgaaa atcaggcatg actccttgcc tggcgctgta
     3541 cgcaatcctc tcccaatcgc ctgaagccag cgaatctctg attttgtcgg gcgtgcgtaa
     3601 atgatgcacc ggacatcact gtcaaatccc gccaccagaa cgccaacgct gacaatgatt
     3661 ttcgtcgctc ccgtttcaaa acgactgatc attaactgac gctcatcatg tggcgtttca
     3721 gcaaccatca cctctgcgtt aattccggct ttgttgaact gcatggtcac gaagctggca
     3781 tgcgttttat tgacgcagaa cgccactgtc ggtaaatccc ttccgttaca cagccagtta
     3841 tcgacaatat cgccaaccag atccgcgccg cacataatct ctgccagttg tgattcgtcg
     3901 taatccctgc cgtactccat tgagactttc gtttttacac cttccagatc cggtttcgtg
     3961 ggcgcataaa attcgtaatt gctcagttcg ccacgctgga tcagttcccc tatagtggtc
     4021 ggcttgagaa gacgctggta ataacgcccc aggaacgatg aaaagggtgt accggacaac
     4081 ccaatcacct tcacatttcc ctctgcaacc agtcgttcga tttccgtgag gatgcggcgc
     4141 ttacggagat gcgcttcatc aataatcagc aggtcgatat tctgaggaaa atcacggcga
     4201 atcagcgtat ccgcactggc aatctgaatc agtcgtgacg ggtcgttatt gggatgatca
     4261 cgccagataa agctgatttc atccccggaa agcccgtact gaacaaagcg ctgtgctgtc
     4321 tggttgatca aaatggtata aggaacgaca aacagaacgc gcatgccacg gcttaccaga
     4381 ccagcagtga tgaacgccgc caggccagtt ttaccactcc ctgtaggcat gtacatcatg
     4441 aaggactgat atgttttcca gtcatgacgc aacatattca gcgcacgttc ctgagcaaaa
     4501 ttcggcttga tattcagcat ggtattctcc cgatgacagg gctgatacag tgattactta
     4561 aaccgggtcg cccggatgac atccacacca tcggcgatat aacgctgtgg atgtttgcca
     4621 tcacggagaa aaacaaatcc cgaaatgcct tcaggaagcg ctcgaatctt catcagtgaa
     4681 cgctgacgag gcgatcggtt atccacctca actgcgcaac gctgaccatc gggagaaata
     4741 accagaagat cgataaatcc cttgcgatca cctttcagcc tgacagtgaa acagcgttgc
     4801 aactcacagc ccagggattt gattttcttt tgcagaacgt agtcgaatgt gaccttgtcc
     4861 attccatgtc cgaattcctc ttccagaagg ttgattagta ccagtttcag ttcagcactc
     4921 atattttaac catgatgtac tagtttgctg atgcagccgt tttttcaggg gtgcccccct
     4981 ttagatcgag atctacctaa cctctgtacc cgtctgttgg aagatcccgt tccagtgctt
     5041 cgca
//
LOCUS       DAZGSP010000200         4974 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZGSP010000200 DAZGSP010000000
VERSION     DAZGSP010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.202"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(344..1375)
                     /gene="rsmC"
                     /locus_tag="ACHK74_005014"
     CDS             complement(344..1375)
                     /gene="rsmC"
                     /locus_tag="ACHK74_005014"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFP0223679.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFIVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1478..1891
                     /gene="holD"
                     /locus_tag="ACHK74_005015"
     CDS             1478..1891
                     /gene="holD"
                     /locus_tag="ACHK74_005015"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFP0223680.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1860..2306
                     /gene="rimI"
                     /locus_tag="ACHK74_005016"
     CDS             1860..2306
                     /gene="rimI"
                     /locus_tag="ACHK74_005016"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP0223681.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2321..2998
                     /gene="yjjG"
                     /locus_tag="ACHK74_005017"
     CDS             2321..2998
                     /gene="yjjG"
                     /locus_tag="ACHK74_005017"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFP0223682.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3383..4618
                     /locus_tag="ACHK74_005018"
     CDS             3383..4618
                     /locus_tag="ACHK74_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0223683.1"
                     /translation="MALSDAWLRSVVGKKRDKVLVKSDRDGLSVRVSPKGRVVFQYRY
                     QWAGKGERLDIGTYPATGLKEAREEVIRLRGELELNRNPRLVKQAEKRKATEAMTVES
                     VIRAWYEAYCVKNKKGSEQILRSFELHLFSKIGNIPHDAATLHDWLEVLEPLSTKTPA
                     IVDRLLINAKQAHVWAYKRKLIETRPLSDITGKDMDIRKGQKKRFLTHDEINILYAAI
                     DGSRMVPKYRAFIKLLLHFGCRSSELITARVDDFDFINKVWTVPPERHKTGDITGEPL
                     KRPIIEPVEELIKYVISMNNGSDMLFTKEGSREPVGRTSLQSLPYNLMQFAWRRLGYQ
                     FPHWSLHDLRRTARTNFSDLTAPHIAEIMLGHKLPGVWQVYDKSDYLEEQRKAYWAWW
                     ERVESIVTCTDLISNGQFA"
BASE COUNT         1264 a         1191 c         1269 g         1250 t
ORIGIN      
        1 gggggacttg aacccccacg tccgtaagga cactaacacc tgaagctagc gcgtctacca
       61 attccgccac cttcgcatac catcaattct taaaaagaat tgctaccacg gaggcgcatt
      121 ctagtggttt tcagcttttc gtcaatagtt aattatcgcc ggaggtgtaa ttgctggaaa
      181 aatgtccatc aggaaactag cgtgcaggtt tggtaggcat gcgggggcag atgccagatg
      241 cgacgctggc gcgtcttatc tggcctacga agggctaacg tgcaggtttt gtaggtcgga
      301 taaggcgttc acgccgcatc cgacacggta ttcggcgaga taattaacct ttcttcgcct
      361 ggcgggtcat aatggcgcga tacaccttga aacgcccggt ttgcgcaatc acttcatgga
      421 agccaaatgt ctcatccagc acgtccgggt aaggcaggaa ggcgttcgct acaattcgca
      481 gctcgccgcc gctattaaga tgacgcaccg cgccgcgaat cagcgtttgc gccgcatcca
      541 ggctggtttg catcccatcg tggaacggcg ggttggagat gatcatatca aaacgacctt
      601 tcacctcgga aaagacgttg ctggcaaaga cttcaccttc aacacagttg gccgcaagtg
      661 ttgcgcggct ggcttctacc gctggcgcag agacatcgca caaggtgaga cgaattttcg
      721 gcgaatggcg cgcaaaggca actgaaagca cccccgcgcc acagccgaca tccagcactt
      781 tacctttcgt gtgcggagtt aacgtcgaga gcagcaactg gctaccgaca tccagaccgt
      841 cgcggctaaa cacgccaggc agcgttttga ccgtcaggcc atcgacgctg tattcgcccc
      901 agaatttatc ggcatcaaat accggctgtt tttccagacg accaaaatag aggccacagc
      961 gacgggcgct gtcgacttta ttcaacggcg cataatcagc cagcatctgc tcggcgctgc
     1021 gcacgccgct gcgattctcg ccaacgataa aaatatctgt ccccactggc agcagagaaa
     1081 gtaaattcat caactggaac tgggcttccg gtttgttctt cggccagtag taaatcagcg
     1141 tatcgcaatc tgcgacgtca tccgccgtgg cgaccagact gaaacgggcg ttatccccca
     1201 tctggcggct taatacctgc cagtggtgga attgctgggt atgagcacgg ctggccgcgg
     1261 tatctaaacg cgcgggcagg tcatcctgta agtctccggc aaacagaata cggctttgtt
     1321 cgaaatcatc actgtgacgc agcaagactt cacttgccgg ggtaaatgca gacatggaat
     1381 gctcctcaat tgatactggc ggcgattata gccatatgtt ggcgcggtat cgacgaattt
     1441 gctatatttg cgcccctgac aacaggagcg attcgctatg acatcccgac gagactggca
     1501 gttacagcaa ctgggcatta cccagtggtc gctgcgtcgc cctggcgcgt tgcaggggga
     1561 gattgccatt gcgatcccgg cacacgtccg tctggtgatg gtggcaaacg atcttcccgc
     1621 cctgaccgat cctttagtga gcgatgttct gcgcgcatta accgtcagcc ccgatcaagt
     1681 gctgcaactg acgccagaaa aaatcgcgat gctgccgcaa ggcagtcgct gcaacagctg
     1741 gcggttgggt actgacgaac cgctatcact ggaaggcgct caggtggcat caccggcgct
     1801 caccgaatta cgggcaaacc caacggcacg cgccgcgtta tggcaacaga tttgcacata
     1861 tgaacacgat ttcttccctc gaaacgactg atttaccagc ggcttaccac attgaacaac
     1921 gcgcccacgc ctttccgtgg agtgaaaaaa cctttgccag caaccagggc gagcgttatc
     1981 tcaactttca gttaacgcaa aacggcaaaa tggcggcgtt tgcgattacg caagtggtgc
     2041 tggatgaagc tacattgttc aatattgcgg tcgatcctga ctatcagcgt cagggattgg
     2101 gaagggtgct gctggaacat ctgatcgacg aactggaaaa acgcggcgtg gcgacactat
     2161 ggctggaagt ccgtgcctca aatgctgccg ccattgccct gtacgaaagt ttaggcttta
     2221 acgaggcgac gattcgccgc aattactacc ccaccacgga cggtcgcgaa gacgccatca
     2281 tcatggcgtt gccaatcagt atgtaagaca aggtggaata atgaagtggg actggatttt
     2341 ctttgatgcc gatgaaacgc tgtttacctt tgactcattc accggcctgc agcggatgtt
     2401 tcttgattac agcgtcacct ttaccgctga agattttcag gactatcagg ccgttaacaa
     2461 gccactgtgg gtggattatc aaaacggcgc gatcacttca ttacagcttc agcacgggcg
     2521 ttttgagagc tgggccgaac ggctgaaagt tgaagcaggc ttgcttaacg atgcctttat
     2581 taatgcgatg gcggaaatct gtacgccgtt gccgggcgcg gtttctctgc ttaacgccat
     2641 tcgtggcaac gctaaaatcg gcatcatcac caacggtttt agcgccttgc agcaagtgcg
     2701 tctggaacgc acgggcctgc gtgattactt cgatttgctg gtgatttccg aagaagttgg
     2761 cgttgccaaa ccgaataaga aaattttcga ttatgcgctg gaacaggcgg gcaatcctga
     2821 ccgttcacgc gtgctgatgg ttggcgacac tgccgagtcc gatattctcg gtggcatcaa
     2881 cgccgggctg gcgacctgct ggctgaatgc gcaccatcgc gagcaaccag aaggcatcgc
     2941 gcccacctgg accgtttcat cgttgcacga actggagcag ctcctgtgta aacactgatt
     3001 gcctcccccc gttgatgggt aaaatagccg caatttttcg ttttcaacaa gcgcggcgcg
     3061 atgccgctta ctcaagaaga aagaattatg acgttgtctc cttatttgca agaggtggcg
     3121 aagcgccgca cttttgccat tatttctcac cccaataatt aagcctaaat taaagctctt
     3181 ttactttttc aaagtccttt cagttaattg atgttttgtt ttataagtca tttaaataca
     3241 gttagttatg tggcttcttt aattgaaaat tctttcatgt aactttaaat cgatcaggtt
     3301 gctttcatca aaaatctgta catatgcttg tacataatgt acaaagcaac agaggtgttt
     3361 tgcgatttgt acaaggtgag taatggcgct gtctgatgcg tggttgcgtt cagtcgttgg
     3421 aaagaaacgt gataaggttt tggttaaatc cgatcgtgat ggtctgtctg tcagagtatc
     3481 accgaaaggt cgcgtagtgt tccaatatcg ttatcaatgg gcagggaaag gtgagcgcct
     3541 tgatatcgga acttacccgg caactggatt aaaagaggcc agagaagaag ttatccgtct
     3601 ccgtggtgaa ctcgagttaa accgcaatcc acgattagtc aagcaggctg aaaaacgaaa
     3661 agctactgaa gccatgacgg tagagtctgt gatccgtgcc tggtatgaag catattgtgt
     3721 aaaaaataaa aaaggttctg aacagatact ccgctcgttt gagctgcacc tgttctctaa
     3781 aatcgggaat atccctcacg atgcagctac attgcatgat tggttagaag tcctggagcc
     3841 tcttagcacc aagactccag caatagtaga ccgattgcta attaacgcaa agcaggctca
     3901 tgtctgggcg tataagagaa agctcattga aactcgccca ctgtcggata tcacgggtaa
     3961 agatatggat atccgtaaag gtcagaaaaa acggtttctg acacatgatg aaattaacat
     4021 cctttatgct gcgatcgatg gttctcgaat ggttcctaaa taccgagcct tcattaaact
     4081 attgctgcat tttggttgcc gtagttcaga gctaattact gctagggtgg atgattttga
     4141 tttcataaat aaggtatgga ctgtaccacc agaacgacat aagactgggg acataacagg
     4201 cgaaccgcta aagcggccca ttattgaacc ggttgaagag cttataaaat acgttatctc
     4261 tatgaacaac ggttccgata tgctttttac caaggaagga agcagggagc cagttggtcg
     4321 gacatcattg cagtcgctgc cttacaattt aatgcagttc gcatggcgac gtttggggta
     4381 tcaatttcct cattggtctc ttcatgattt gagacgaaca gcacgaacaa acttttctga
     4441 tcttactgct cctcatattg ctgaaataat gctcggtcat aaactgccag gtgtatggca
     4501 agtttatgat aagagcgatt atctagaaga acagcgtaaa gcttactggg catggtggga
     4561 gagggttgaa tcgatcgtta cttgtactga cttaatctcc aacggacagt ttgcgtagcc
     4621 agagcgtaat catatctgcc agtccgctgc ggataccttg tgtactgggt ttttagccag
     4681 actacgttaa ccggtagctt cagtatttgg ggggtcggca ggagtttgat ggtttattgg
     4741 gttactgtgg gactaaggat gtagggatgc caatcaatga attatgaaaa atatcatagc
     4801 acaaacagaa gcaagggttt gcgaaccttc tgctacttaa tttattatgt gcaggtgatt
     4861 tttaatgtga tgaagtgttt tatgactcgc ggaaactttg attttataaa ctctaacgtg
     4921 gttttctcag aagccgataa gtcattgtgg attgatctca ttaacaccga ttgt
//
LOCUS       DAZGSP010000201         4955 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZGSP010000201 DAZGSP010000000
VERSION     DAZGSP010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.203"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            234..1208
                     /gene="hemB"
                     /locus_tag="ACHK74_005019"
     CDS             234..1208
                     /gene="hemB"
                     /locus_tag="ACHK74_005019"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFP0223684.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1314..2165)
                     /gene="tauD"
                     /locus_tag="ACHK74_005020"
     CDS             complement(1314..2165)
                     /gene="tauD"
                     /locus_tag="ACHK74_005020"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFP0223685.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2162..2989)
                     /gene="tauC"
                     /locus_tag="ACHK74_005021"
     CDS             complement(2162..2989)
                     /gene="tauC"
                     /locus_tag="ACHK74_005021"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFP0223686.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2986..3753)
                     /gene="tauB"
                     /locus_tag="ACHK74_005022"
     CDS             complement(2986..3753)
                     /gene="tauB"
                     /locus_tag="ACHK74_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFP0223687.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3766..4728)
                     /gene="tauA"
                     /locus_tag="ACHK74_005023"
     CDS             complement(3766..4728)
                     /gene="tauA"
                     /locus_tag="ACHK74_005023"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0223688.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQRGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1166 a         1412 c         1249 g         1128 t
ORIGIN      
        1 gaattattca atttatagca gcacaaccaa aggccaaaaa ataataacca agagctataa
       61 ataaagatta aaaatatttt cagtgaatat gaaattattg ctatttctat tacaaagatg
      121 tcctggtctc tctttcattc tcttcttgtc aacatcacga caactttcgt aaaacactcc
      181 tgccccgctt caggtatact gtgcacctta attccacaaa catcaggcag accatgacag
      241 acttaatcca acgccctcgt cgcctgcgca aatctcctgc gctgcgcgct atgtttgaag
      301 agacaacact tagccttaac gacctggtgt tgccgatctt tgttgaagaa gaaattgacg
      361 actacaaagc cgttgaagcc atgccaggcg tgatgcgcat tccagagaaa catctggcac
      421 gcgaaattga acgcatcgcc aacgccggta tccgttccgt aatgactttt ggcatctctc
      481 accataccga tgaaaccggc agcgacgcct ggcgggaaga tggactggtg gcgcgtatgt
      541 cgcgcatctg caagcagact gtgccagaaa tgatcgtcat gtcagacact tgtttctgtg
      601 aatacacttc tcacggtcac tgcggtgtgc tgtgcgagca tggcgtcgac aacgacgcga
      661 ctctggaaaa tttaggcaag caagccgtag ttgcagctgc tgcaggcgca gacttcatcg
      721 ccccttccgc cgcgatggac ggccaggtac aggcgattcg tcaggcgctg gacgctgcag
      781 gctttaaaga tacggcgatt atgtcgtatt cgaccaagtt cgcctcttcc ttttatggtc
      841 cgttccgtga agctgccgga agcgcattaa aaggcgaccg caaaagctat cagatgaacc
      901 caatgaaccg tcgcgaggcg attcgtgaat cactgctgga tgaagcccag ggcgcagact
      961 gcctgatggt taaacctgct ggagcatacc tcgacatcgt gcgtgagctg cgtgaacgta
     1021 ctgaattgcc gattggcgcg tatcaggtga gcggtgagta cgcgatgatt aagttcgccg
     1081 cgctggcggg tgctatagat gaagagaaag tcgtgctcga aagcttaggt tcaattaagc
     1141 gtgcgggtgc ggatctgatt ttcagctact ttgcgctgga tttggctgag aagaagattc
     1201 tgcgttaatt tttttcctct cagaaagggg gagttaacct gttgaaagta cggtgaacgc
     1261 cccctcaccc taaccctctc cccaaggggg cgagggaacc gtccgcactc gtatcacccc
     1321 gcccgataaa acggtttatc cccaaggatc gtcgcccgat gcataatccg ccgctgtggc
     1381 aggtaatcgg cattggcata gtgctgagtc acgcggttat cccaaatcgc aatatcattc
     1441 ggttgccagc gccaacgcac ctgaaactcc ggtttagtga tatgggcgaa taaaaagcct
     1501 aacaaggctt cgctctcttt ctcgctgaca tcgacaattc gcgtagtaaa gccttcattc
     1561 acaaacagcg cctgtttacc gctcaccgga tgcgttcgca ccaccggatg tagcaacgga
     1621 ggatttttcg cgaccgcctc gcgccagcgt tgatgttcct cctcggtttt gcggtatttg
     1681 tattccggga acgatttacg gaaatcatgc tccgcacgca gcccgctcag cagctggcgg
     1741 aagggaacag agagcgcctc ataggccgca ataccgctgg tccagagcgt atcaccgccg
     1801 gtcgaaggta actctttagc tgccagaatc gcccctgcgg gtggcgtttc aataaatgtc
     1861 acatcggtat gccagttgtc gttatccggc ggattatcgt tatgggtatc cagcacgatg
     1921 atctcgtcaa ccccttcggc atgagggtaa acagggtgaa tatgcagttc gccaaaacgc
     1981 tgggccagcg cgcgttgctg ctgcggcgta atagtttgat cgcgcagaaa caccacctga
     2041 tggcgcagca ccgcatggta aagctgttca aactgattat cgcttaacgg gcgcgtcagg
     2101 tcggcacccg aaatttgtgc gccgatatac ggccccagcg gggtaatgct cagacgttca
     2161 ctcattgtac ttctccatgc cagggcgtca gacggcgctg taacgcgcgc aaacccagtt
     2221 ctaaaagaaa ggcgataatc gcaatcaccg cgattcccgc cagcaccacg tcagtcgcga
     2281 gaaattcacc cgctgactga accataaatc ctaaaccgcg cgtcgcggca atcagctccg
     2341 ccgctaccag cgtagaccag cccacaccca gaccaatgcg taatccggtg aggatttccg
     2401 gcagcgcgcc gggcaaaatg acaaaccaca gcacctgcgc acggctggca cccagcgact
     2461 gggcggcacg aatgcgaacc tgctgcgcgc ttttcacccc cgccagcgcc gacatcgcca
     2521 ccggtgcaaa aatcgctaaa tagatcagta agatcttcga ggtttcacca ataccaaacc
     2581 agatcaccat cagcggcaaa taagccagcg gcggcacggg acgataaagt tcgattaccg
     2641 gatccagaat gccgcgcacc gtcgggctta gccccatggc gatcccgacc ggaataccga
     2701 tcaccaccgc cgcgagtagc gccagcacaa tgcgcgtcag gctggctgcc agatgctgcc
     2761 acagcgtggc gtccataaat ccctgcggtc cggcaatggt gagcagtttt gccagtactt
     2821 gttgcggcgg cggcaaaaac agcgggctaa tcagttgcag cgccgccacc gtccaccata
     2881 cagtgagtaa aaccgctaac gtgccaatgc ttaaggtcac ctgacgcgag agcggccagc
     2941 gccatttcag ccgccgcgaa tgcagttttt cattaatgag cacactcatg agaacgcctc
     3001 ccgttgctca aatacgcggc ttaaaacata ttcgcgcatg gcgatgaatt gtggatcgga
     3061 cttgatgctg cggctcgact ctcccgcaac aaagcggcga gcaaagttga gcggcagccg
     3121 ctccagcaca cggccagggc cggatgaaag cagaaccagt tcagtcgcca taaacaccgc
     3181 ttcttctata tcgtgggtaa tcaacagcac ctgcttgccc gtctcctgcc agagtttcag
     3241 cagcagagtt tgcatctggt cgcgggtgaa ggcgtccagc gcaccaaacg gttcgtcgag
     3301 taataacagc tggggattcg ccgccagcgc gcgggcaatc cccacccgct gacgttgacc
     3361 accggaaagc tgccagatat agcgtttttc tgcgccttcc agccccactt ttttcagcat
     3421 ctggtgcgcg atttccagtc gctgcatttt ctctatacct gccaattgca ggccgaacgc
     3481 cacgttgtcc tgtacattgc gccacggtag tagcccttca ttctgaaaaa ccaccccgcg
     3541 ctctgctccc ggtccctcaa tacgcttacc cgccagttga atgctgccat gctgataagg
     3601 cacaaaaccg gcaatcagat tcagcagggt ggttttaccg cagccggacg gccccagcac
     3661 caccagtagc tcgccgcttt ccagcgtcag gttgatatct tccagtgccg gtttgccgcc
     3721 ataatcggca tagagatgag agatttgcag catctgcgcc tccttttatt gcacgaagcg
     3781 cgaggtaacg tactggctgt aatcattcgc tacagccggg accttgccct gctctttcaa
     3841 aaactgcgcg gtgtcgatga ttgctttgtt caccggtccg gtcagttctg ccgtttgttg
     3901 ctgcggcgtc agataggtat ttcccttcac cagccccgga acgtcacctt caggcacgcc
     3961 gcttaaacgc gccagtttgc tgatgttttc cggctgtttc agccacgcgt ctgggttagc
     4021 aatgtacggt tgctgagcat cgatggcgct tttagcgaac gctttcacga cctcaggatg
     4081 tttctcggca aaatctttgc gcaccaccca gacgtccagc gttggcgcac ccctctgccc
     4141 gacctgttca gaatcggtca acaccttgcc gtctttttcc agggcgttaa ccgccggtgc
     4201 ccagacataa gcaccatcaa tatctccccg ctgccaggca gcgataatcg cgggcggctg
     4261 caggttcaca atctccactt gcccgggttt aatgccccag tgtttcagcg ccgccagcag
     4321 gctgtagtgg gtggtagaga taaacggtac ggctatgcgt ttgccaatca gatcttccgg
     4381 tttgctgata gttttcttta ccaccagcgc ttcggagtta cccagttttg acgccagcaa
     4441 gaaaacttca atcggcacct gttggctggc tgcaaccgct aacgggctgg aaccgaggtt
     4501 gccgatttgc acatcgccag aagccagcgc ccgcacgatg ctggctccgc tgtcaaactt
     4561 acgccagtcc acggttgctc cgctttcttt agcaaaggtg ttgtcggcct gagccacttt
     4621 cgccggttcg gctgaggttt gatacgccac ggtgacgttc accgcctgtg cctgaaaagc
     4681 gatgaatgcc agtgcggcaa gaagtgtgtt acgcgatgaa attgccatga ttgtctgctc
     4741 ccctgtcttg ttatgggagc agtattcagg aataaaaaca ttcattaaaa gaattagtcg
     4801 ttatcgcaca gatgatttta ttcttagcaa aaaaacggct aaggaaagat taaatatgca
     4861 attactccgt tctaacaatt gggaaaatta ctacacttca ttttagtgat gcataaacaa
     4921 ccacattacg agatttatta cttcattatt tctat
//
LOCUS       DAZGSP010000202         4869 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZGSP010000202 DAZGSP010000000
VERSION     DAZGSP010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.204"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            184..351
                     /gene="rmf"
                     /locus_tag="ACHK74_005024"
     CDS             184..351
                     /gene="rmf"
                     /locus_tag="ACHK74_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFP0223689.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(421..939)
                     /gene="fabA"
                     /locus_tag="ACHK74_005025"
     CDS             complement(421..939)
                     /gene="fabA"
                     /locus_tag="ACHK74_005025"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFP0223690.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(1008..2768)
                     /locus_tag="ACHK74_005026"
     CDS             complement(1008..2768)
                     /locus_tag="ACHK74_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0223691.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDGSRPLPVSVPSMPLRLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            2954..3406
                     /gene="matP"
                     /locus_tag="ACHK74_005027"
     CDS             2954..3406
                     /gene="matP"
                     /locus_tag="ACHK74_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFP0223692.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(3482..4522)
                     /gene="ompA"
                     /locus_tag="ACHK74_005028"
     CDS             complement(3482..4522)
                     /gene="ompA"
                     /locus_tag="ACHK74_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFP0223693.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
BASE COUNT         1255 a         1232 c         1212 g         1170 t
ORIGIN      
        1 aaaatttgtt cctcttcaca ttttttgtac aaccgacatg cccgtgtagc tcacaaatat
       61 gacagtggcg tgaattttgc gcattgacgg cagttatgat tcgcggtatt gcttaactgt
      121 gattgcacat ttagtaatca ctgttttctt ttccaccaga aaccagtatg agggaaacga
      181 ggcatgaaga gacaaaaacg agatcgcctg gaacgggcac atcaacgtgg ttatcaggcc
      241 ggcatcgccg gacgctcaaa agaaatgtgt ccctatcaga cgctgaatca aaggtcacaa
      301 tggctgggag gctggcgaga agccatggcg gacagggtag taatggcctg attctgtctc
      361 tttaaaaaga aacctccgca ttgcggaggt ttcgcctttt gatactctgt ctgattataa
      421 tcagaaggca gacgtatcct ggaacagacc gactttcagg tcgctggcgg tatagataag
      481 acgaccatca accagcactt cgccatccgc caggcccata atcagacgac ggttaacaat
      541 gcgtttaaag tgaatacggt aggtcacttt tttcgctgtc ggcagtacct gaccagtgaa
      601 tttcacttcg ccaacgccca gcgcgcggcc tttaccttcg ccgcccagcc agccgaggta
      661 gaaccctacc agctgccaca ttgcgtccag gcccaggcat cccggcataa ccggatcgcc
      721 aataaagtgg catccgaaga accacagatc cggattgata tccagttctg cttcaacata
      781 ccctttgtcg aagttaccac ccgtttcggt cattttgacc acacggtcca tcatcagcat
      841 gttcggtgct ggcaattgcg ggcctttagc gccaaacagt tcaccgcgac cagaggcaag
      901 aaggtcttct tttgtatagg attcgcgttt atctaccatg ttctctgtaa gccttatttt
      961 attgaagcac gcaggatagc taacacgtgt acgctgaaca agtccgatca gttcggaata
     1021 aaccagttca gccaacgtaa tggccatgga aaacggtgac gtccttcctg ttgcgatgct
     1081 tgcgcgatac gttcctggat ggtttgcatc agcgtcgttt ggccttcgcc atcccacacc
     1141 agatttaata ataacggcag tgcgtcagtc acatcgtcta ctgcccagat ggtgaatttg
     1201 ccttcttcta ccgctttcac cagttcactg tgaagactta aatggcgaac gttagctgtg
     1261 gggataatga caccttgttt cccggttaac tcacgttgct ggcaaatagc aaagaagcct
     1321 tcgattttct catttaaacc accgaccggc tgggcgcgac cgaactgatc gactgaacct
     1381 gtgatagcga tactctgatt caccggcaca tcggcgaggg cgcttatcag ggcgcagagt
     1441 tcagccatcg aggcactatc tccatcaact tcactgtatg actgctcaaa tgtcagcgat
     1501 gctgagaagg ggatctgttg ctcaagctgt agttccgaca tcaggaacgc ttgcatgatc
     1561 atcatccctt tcgcatggat attgccgcca agctccgctt tgcgttcgat gtcggtgaat
     1621 tcaccatcgc caatatgcac aacgcagcta atgcgagaag gttcgccaaa agcgcgtgga
     1681 tgacccggaa attcaatgac cgaaagggcg ttaatttgcc cgatgcgttc gccttcggtt
     1741 tcaatcagga tttgctcctg aaggatctca tcctgcatac gttcagcgag gaaaccttcg
     1801 cgccattcac gctgctgcag cattaagttt agctgctcgc cggagaaggt gtcgccatca
     1861 cacagggagg cgacctcttt acactggcgg aggatccact gcgggctaag cggaagtgtt
     1921 tcttgttcac cggtgtagcg tgctgcttcg cggataagta ccggccaggc atccgctccc
     1981 ggtgcaggta agtgattatg tctggcggta aatgtcaccc agcgacacca ctgggttact
     2041 gactccgcat cgacaatctg cagagtatct tcaaattcgc tataaatagc ctgctctgaa
     2101 agctctggct ccatctcctg gaaatcagcc aatgattcgc gttcgcctac cagaatgact
     2161 ttcagcctca atggcatcga aggcacagag acggggagag ggcgcgaccc atcaaacgca
     2221 acccagtcaa aacgctcgcg gttaacgata tttttcagcc gcatccacag cagaggttgc
     2281 gccagcagtg tacgcaaaga gatgatgaga ataccgccat ttgcctgatg caccagacca
     2341 ggctgcaggg taatgtcgcc attaaactgg cgcaggcagc caaagagttg ctccgcttct
     2401 acccagtcgg cagcgacaac ttgcgttaaa gtcgcaaaat tatcatctgc actcactgcg
     2461 tgacgtaagc ggatggagtg gccggaaacc tcatagtgac cgcccaccag ttggcctgca
     2521 tcgctttgta gcgtacgcgc ggcattggca ataagattca gatactcaga ctcttccggg
     2581 gccttcgcca gcataaaaga ggaggatgct cgcgtatgca gcaactgctc cagcgcaaat
     2641 tgcagccgcg gttgagtatc actgaataaa ggatcgtttt cgtcaatcaa atgtggctga
     2701 gcaaatattt cctgatagct atcggtatca ggaaccaggt cacgccatgc aagtttcgta
     2761 atggtcaaag ttgatgtttt ttagtctgtt gtcaaagccg cgattatacc gtaaccggca
     2821 ctacagcaca cgtagaaagc accgacaata ctcctggcat gggcgttaaa gctcacagga
     2881 tggagattct tttcttcact ggcctaaaaa gctgatattc tgtaaagagt tacacggtaa
     2941 cattgagatc gctatgaaat atcaacaact tgaaaatctt gaaagcggtt ggaaatggaa
     3001 gtatctggtt aagaagcatc gcgaagggga gttaatcacc cgttacatag aagccagtgc
     3061 cgcccaggaa gccgtcgatg tgttgctctc gctggaaaat gaacccgtgc tggtaaatgg
     3121 ctggattgac aagcatatga atccggaact ggtcaatcgc atgaagcaaa ccattcgggc
     3181 aagacgaaag cgccatttta atgcggaaca ccagcatacg cgcaaaaaat cgatcgatct
     3241 ggaatttatt gtctggcaac gtctggctgg tcttgcgcag cgtcgcggta aaacgctttc
     3301 tgaaacgatt gttcagctga ttgaagatgc ggaaaacaaa gagaaatacg cgaataaaat
     3361 gtcttctctg aagcaggatc tgcaggcatt gctgggtaag gaataactga cgaaagtcag
     3421 tttaatttac taaaggcaaa aaaaaccccg cagcagcggg gtttttctac cagacgagaa
     3481 cttaagcctg cggctgagtt acaacgtctt tgataccttt aacttcgatc tctacgcgac
     3541 gatccggagc caggcagtcg atcagtgcag cacgctgttt cacgttgtca caggtgttgc
     3601 cagtaaccgg gttggattcg cccataccac gtgcggagat cttgtctgcc gggatacctt
     3661 tggagatcag gtaatcaaca acagactgag cacggcgctc ggacagaccc tggttgtaag
     3721 cgtcagaacc gatgcggtcg gtgtaaccca gaacaactac ggaaccgtct ttcggatcca
     3781 ggttgctcag ctggctgtac agctgatcca gagcagcctg accttccggt ttcagggttg
     3841 ctttgttgaa gttgaacaga acgtcagact tcagagtgaa gtgcttggtc tgtacttccg
     3901 gtgccggagc tggagccgga gcaactactg gagctgcttc gccctgaccg aaacggtagg
     3961 aaacacccag gctcagcatg ccgttgtccg gacgagtgcc gatggtgtgt gcgtcaccga
     4021 tgttgttggt ccactggtat tccagacggg tagcgatttc aggagtgatc gcgtactcaa
     4081 caccgccagc gaagaccgga gaaacgccgg tgtcgtggtt tttaccataa acgttggatt
     4141 tagtgtctgc acgccatacc ataccaccca gacgagtgta gatgtccagg tcgtcagtga
     4201 ttgggtaacc cagtttagcg gtcagttgaa cgccctgagc tttgtatgca ccgttttcaa
     4261 cgctgccttt gtacggcata cgacctaacc agtcgtaacc catttcaaag ccaacatacg
     4321 ggttaacctg gtaaccacca aaagcaccag cgcccagttg gttttcatgg gtcgggccat
     4381 tgttgttgat gaaaccagtg tcatggtact gggaccagcc cagtttagca ccagtgtacc
     4441 aggtgttatc tttcggagcg gcctgcgcta cggtagcgaa accagccagt gccactgcaa
     4501 tcgcgatagc tgtctttttc attttttgcg cctcgttatc atccaaaata cgccatgaat
     4561 atctccaacg agataacacg gttaaatcct tcaccggggg atctgctcaa tattaactct
     4621 accgatatct tcggcttatg ccgagcaccc ctggcgatgt aaagtctaca acgtagttga
     4681 aaacttacaa gtgtgaactc cgtcaggcat atgaaaaaaa agtcttgtat aaggtatgtt
     4741 taatcttttt tgtcagcgac aatttacaga agagaatcgc ggaaaccgct tcagacaagc
     4801 ctccgcaagg aaaattagtc acgactgaaa gcattggctg ggcgacaaaa aaagttccag
     4861 gattaatcc
//
LOCUS       DAZGSP010000203         4857 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZGSP010000203 DAZGSP010000000
VERSION     DAZGSP010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.205"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(164..677)
                     /locus_tag="ACHK74_005029"
                     /pseudo
     CDS             complement(164..677)
                     /locus_tag="ACHK74_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
     gene            complement(738..842)
                     /locus_tag="ACHK74_005030"
                     /pseudo
     CDS             complement(738..842)
                     /locus_tag="ACHK74_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(844..1167)
                     /locus_tag="ACHK74_005031"
                     /pseudo
     CDS             complement(844..1167)
                     /locus_tag="ACHK74_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1911..2888
                     /locus_tag="ACHK74_005032"
     CDS             1911..2888
                     /locus_tag="ACHK74_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP0223694.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3167..3907)
                     /locus_tag="ACHK74_005033"
     CDS             complement(3167..3907)
                     /locus_tag="ACHK74_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0223695.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLNGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(4028..4249)
                     /locus_tag="ACHK74_005034"
     CDS             complement(4028..4249)
                     /locus_tag="ACHK74_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFP0223696.1"
                     /translation="MWILNVFQVHKSVRIYIAHFSVFNCHLHPSIDRSLASWYYPKNG
                     KPALSPHHDWLKTGSQRQNAPLRIWFYPQ"
     gene            complement(4334..4702)
                     /locus_tag="ACHK74_005035"
     CDS             complement(4334..4702)
                     /locus_tag="ACHK74_005035"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP0223697.1"
                     /translation="MVLALLSRADNFFLSPTELSEKLDISRTNITRVFDSLEKFGFIR
                     RMESKEDRRSKNIYLTPDGDLLLQKITRIYGRYLKKYGATLQMIKSRYLSNQQKDVIS
                     KYFSVISHVIMHINPEFLFL"
     gene            complement(4713..>4857)
                     /locus_tag="ACHK74_005036"
     CDS             complement(4713..>4857)
                     /locus_tag="ACHK74_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0223698.1"
                     /translation="LLNKVIIYQQQNKQSYPAQEHLLLQLCMRVTKKLTDNINSSLKG
                     GYK"
BASE COUNT         1223 a         1173 c         1141 g         1320 t
ORIGIN      
        1 gctttttcgg ttgaaaagaa attgattgaa ttactggaaa aacaatctgg tctccatacg
       61 tctgtggatt gcagtactcc ccttacagag cagagtctgt cgagaaatga cggtgaagac
      121 cagaccagaa gaaacgctga aaatatgttc ggagatgact gaactaaccc agcctggcca
      181 ggggaatatc tcgccattta gttgtataac actgactcaa catttcccgt ttcatttgcc
      241 attcaggggc ggttcctcgc cccgcaaacc acactttccc ttttcccgac tggttcagtt
      301 catcgagtgt tttcattaac ttttcactgt ttttacgggg ctggatttca tcaaataatc
      361 ccggctgcgc tatacccgat ggtgtgaaat cagccagcat gactcctgcc ttcatatagc
      421 ggtacccttc acgccagaca tggtttaagg ctctgcatgc ggcggcaata atgtcccggc
      481 tgtcctgtgt gggcaatgaa agcttttcca cagcggcatt gctgtaacag ggttcagggg
      541 gatgtccgta caaatgtcgt cacctgccgg caatactgac gctccccacg tagtttctct
      601 gcggcccgct ctgcatactg aacaacagcc tggtgcatgg catctttgtc tgtgattcgt
      661 tcaccaaaac tgcgactatg tcaacgccac gatgtttgac cgttatttgc cattttcatg
      721 cccgctaaat actttcgtta ctgatgctgt ccgctttgca ccatttcatc atcactgatt
      781 ggcgttgtgt ttttggtcag cactccagct ttacgtttct cttttaaccg gtaactctct
      841 ccttgttctt tttgacggac gcattttacg tttgggctgg atgtagtaac gcagcatgga
      901 acgtccgccc gtataaccca ttgccttaat ctccgcgaag ataacctcgc tattccagac
      961 attctctgcc aggcgcatgt cgatgtaatc cataaacggt ttcagcttaa ccattttgtg
     1021 gcgggtcttt ctggctggcg gttcagggta tttgaggtag cgtctgaccg tccgttcaga
     1081 gcaaccaatc tgagccgcaa tatcgacaat gtacgcgccc tgctggcgca tttgctttat
     1141 catgtaaaag tcctctctgc tcagcatgtt gatgtccttt ctggtgtgag aaccttaagg
     1201 aaacaacatg ttgggtggag cggacaatcc aaatggtgaa ttaccgtctt atatcactgg
     1261 cgctgacacc tgtagtttgt atacacaatc cctggcattg aaacacgcat atgaggtgta
     1321 cttcggaata tgaccgtgcg ctcaggaagt acggcagatt ccgtcaaaaa ttacctgtaa
     1381 agacagactt cagaaaaaac agtgctcgcc gcagaccggt gaccgggcga agcccgcgaa
     1441 ccggcgagga agcgcaatgg atctgtagtt acagtgagca ggtacgccct gaagccttca
     1501 taatcggtag tgtatgctgt ttttctggaa tgatttatcc acatatccag aggcccgatc
     1561 cagtaaacag atccatgaat gatcaacaaa ggatccatta aagatcccca tatcgctgtc
     1621 aaccttgtca ctcatgggcc gggaccacgt tcacataagc ggtggcatgt tatggataaa
     1681 ctgtaacagg ttcatgataa gctgtattca gtaatgcata tactgaagta agttaatgac
     1741 ataaactatg gtcagtacgt cagactcagt tgttaaatac aggctgcagg tttttattca
     1801 gtcagttagc ggagttctga cacacgattt tctgtttatt cttttactgt ccacaggctg
     1861 gaggctttct ggaaaacgaa aattcagaca tcaaaaaact gttcggcgag gtggataagt
     1921 cgtccggtga gctggtgaca ctgacaccaa acaataacaa caccgtacaa cctgtggcgc
     1981 tgatgcgtct gggcgttttt gtaccgaccc ttaaatcact gaagaacagt aaaaaaaata
     2041 cactgtcacg tactgatgcc acggaagagc tgacacgtct ttccctggcc cgtgctgagg
     2101 gattcgataa ggttgagatc accggccccc gcctggatat ggataacgat ttcaagacct
     2161 gggtggggat cattcattcc tttgcccgcc ataacgtgat tggtgacaaa gttgaactgc
     2221 cttttgttga gtttgcaaaa ctgtgtggta taccttcaag ccagtcatcc cgcaggctgc
     2281 gtgagcgcat cagcccttcc ctgaagcgca ttgccggtac cgtgatttcg ttttcccgta
     2341 ccgatgagaa gcacacccgg gaatatatca cccatctggt acagtcagcc tactacgata
     2401 ctgagcggga tattgttcag ttacaggctg atccccgcct gtttgaactg taccagtttg
     2461 acagaaaagt ccttctccag cttaaggcga ttaatgccct gaagcgacgg gagtccgccc
     2521 aggcactcta cacctttata gagagcctgc cccgggatcc ggcaccgata tcgctggcgc
     2581 ggctgcgtgc acgcctcaat ctgaagtctc ctgtattttc ccagaaccag acggtcagac
     2641 gggcaatgga gcagttgcgc gagattggat atcttgatta cacggagatc cagcgggggc
     2701 ggacaaaatt cttctgtatt cactaccggc gtccccggtt aaaagcgccg aatgatgaga
     2761 gtaaggaaaa tccgttgcca ccttcacctg cggaaaaagt cagtccggag atggcggaga
     2821 agcttgccct gcttgagaaa ctgggcatca cgctggatga cctggaaaaa ctcttcaaat
     2881 cccgctgaac ataaactgta gtcagtgaag agtgctcctt tactgactac agcttatatt
     2941 atcaggtgca gtgagtggtc tgctcactgc agtttatatt cagtttcatg cagtgctgcc
     3001 tgtggctgag ctgccatctg cctgtccctt acgtgagtca ccccgtaacc tgatgctgag
     3061 gcattgctcc cttcataaaa catggtttac tcactacagc ttatctacct gctccagctt
     3121 atgttatgtc tgttctgctg accactgctt atataaggaa gtgccgttac ttcaggggag
     3181 gcagtgtacg caggatctcc gcagcatccc gcccgtcacc tgtgaaaggc actgccagcg
     3241 tggctgccat atccagtgca aacactctgg tataaacctc catagaacgt ggatccctgt
     3301 gaccagccag tgcctggatg actttccggg gctggcggtg atagagcatg tgcatgatat
     3361 agctgtgccg gaaggtgtgt ggtgtgaccg gaatcgaaaa gtgtactccg tcagcttcgg
     3421 cccgtctgac agcctgcttc agccagttgc gcatggtttc gtcggtcacg gcccataatg
     3481 gttcacgacg acggggccgg gtggtgatca tccagctttc catctgcctg acatagctta
     3541 tatctgtcag cggcaccagt cgcacttcat cttttggcgg gcgtccgcgt cgcgcacgca
     3601 ctttttcgga caggatccgc acaaacggtc ttactccatt caggtcaaat gattccggcg
     3661 tcagcatccg ggcttcgcca atacgcattc cggtattcca gagggtggcg aacagcatat
     3721 ggtgacgctg atccggcata tagaaaagaa gggcactcac ttccggggcc agcaggtagg
     3781 ccggtgtggc ccctgtggaa gtggccattc ttctcaggga gagcgctgtt gcaaaatcca
     3841 cccccggcgc aatgggtaac agggagacgc gttctggtga attctgcagg ggaatgacat
     3901 tgttcatgag ttatttttca cgtagcacac gcactgttta tggatacagg tattctgacc
     3961 tgaacgcccg gatccttcca cgacaataaa tcgcagcgcg cggatccttt tgtttgatta
     4021 tcacgcatta ttgcgggtaa aaccaaatac gtaaaggcgc attttgccgc tgggatcccg
     4081 tcttaagcca gtcatggtgc gggctgaggg ctggtttacc gtttttgggg taataccagg
     4141 acgctaatga tctatcaatg gatggatgta agtgacaatt gaaaactgaa aaatgcgcta
     4201 tgtagattct gacagattta tgcacctgaa atacgttcaa aatccacata gcgtaactct
     4261 gattttcctt accagaaaaa agagggggaa taaccagtat ctcccccccc acacacaatt
     4321 gcagaataat aaattataaa aataagaact caggatttat atgcatgatt acatgtgata
     4381 ttactgagaa atattttgag ataacatctt tttgctgatt acttaaatat cttgacttta
     4441 tcatctgtaa ggttgctcca tattttttta gatagcgtcc atatatcctt gtaatcttct
     4501 gtaataacaa atcaccatct ggagtcaggt aaatattctt actacgtcga tcttccttac
     4561 tttccatacg cctaataaaa ccaaattttt ctagagaatc aaaaactctg gtgatatttg
     4621 ttctggaaat atctaatttt tcactgagtt cagttggaga taagaaaaaa ttatcagctc
     4681 tggataagag ggccagaacc ataagctttg tatcatttat accccccctt cagggatgaa
     4741 ttaatattat cagtaagttt tttggtaaca cgcatacaaa gttgtaaaag gagatgctcc
     4801 tgagctggat atgattgctt attctgttgt tgatagataa taactttatt aagtaat
//
LOCUS       DAZGSP010000204         4836 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZGSP010000204 DAZGSP010000000
VERSION     DAZGSP010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.206"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            57..1754
                     /locus_tag="ACHK74_005037"
     CDS             57..1754
                     /locus_tag="ACHK74_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFP0223699.1"
                     /translation="MEHQLNKKLLNSSISLILASGLMMSTANAADDVKLKATKTNVAF
                     ADFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDDSSFDPKTMDNRNVVDTYKIGIDKAI
                     EAAKKSTPTLAALMDEGVKFTNGYVAHGVSGPSRAAILTARAPARFGVYSNSDAQDGI
                     PLEETFLSELLQNHGYYTAAIGKWHLSKISNVGVPEEKQTRDYHDNFTTFSAEEWQPQ
                     NRGFNYFMGYHASGVSLYNSPSLFRNRENIKAEGYISDQLTDEAIGVIDRASKLEQPF
                     MIYLAYSAPHLPNDQPAPKNYQEKFNTGSKTADNYYASIYSVDQGVKKILNTLKEKGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQAGYKGQPYPGGMHTPMFMWWNGKLKTGDYDKL
                     VSSMDFYPTALDAADIKIPASLKLDGVSLLPYVQKGADKKSAAGEPHKYLSWITSYSH
                     WWDEENTAFWNGYHKFVRKESDDYPHNPHTEDLSQFAYTVRSNDYSLVYTYAENKLAL
                     YDINDLQQTKDLSASHPDVVKNMKAVMIDFITSSQKPISDINQAKFENIKKSLTNVK"
     gene            1823..2080
                     /locus_tag="ACHK74_005038"
     CDS             1823..2080
                     /locus_tag="ACHK74_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223700.1"
                     /translation="MNKHENKKYCSSDEIWCTQRKELIKSVLETKKASDLEKQSTDIA
                     QQYLSETIQFSIDDFIKNIKRKVKSRGSRPPAKRKNTHKGT"
     gene            2301..2516
                     /locus_tag="ACHK74_005039"
     CDS             2301..2516
                     /locus_tag="ACHK74_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0223701.1"
                     /translation="MSQQKITGIDLAKTNFYLFSINAHGKPAGRIKLSRNQLLNWLVQ
                     QPKMTVAMEARGHPITGPGKSGNSTMM"
     gene            2690..2947
                     /locus_tag="ACHK74_005040"
     CDS             2690..2947
                     /locus_tag="ACHK74_005040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223702.1"
                     /translation="MERTQLINHVRGLLAEYGIVFSKGATELRQKLPALLEDAENELT
                     DTMKTLLHRQYIRLITLDNELEWYDSELKNMSAKILCANAC"
     gene            2929..3120
                     /locus_tag="ACHK74_005041"
     CDS             2929..3120
                     /locus_tag="ACHK74_005041"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0223703.1"
                     /translation="MCKRLLAIPRFSPLVSQAIKSWMGDGKQFRRGRDASATLGLVPR
                     QFSTGGKQVLLGITKFGEM"
     gene            3262..4281
                     /locus_tag="ACHK74_005042"
     CDS             3262..4281
                     /locus_tag="ACHK74_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP0223704.1"
                     /translation="MKYTPVGVDIAKHLIQVHFIDEHTGEVIDKQLRRQDFLMFFCNR
                     EPCLIGMEACRGSQHWGRELTKLGHKVRLLQAHFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKDIAVKTEEQQSVLVLHRSRRQLVKFRTMQINSLHGTLLEFGKTIHKGRAAMEREL
                     PEALERMKERLPPYLITVLENQYNRLNELDSLIEDIEKKLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMREASAFKSGREFAAYVGLVPKQTGSGGKIRLLEISKRGNTYLRAL
                     FIHSARAAALLPKEPGPWITELKKRRPTSVAIVAMANKLARTVWAIAAHGSKYDKNHV
                     SIRSY"
BASE COUNT         1531 a         1014 c         1089 g         1202 t
ORIGIN      
        1 atcagaggct tttattatta ataaatgttc gtgatgcaac tcagaaggaa taccatatgg
       61 aacatcagtt aaataaaaaa ctgcttaact catcaatatc acttatatta gcatctggtc
      121 tgatgatgag tactgcaaat gcagcagatg atgttaagct caaagcaaca aaaaccaatg
      181 ttgcctttgc ggacttcacg ccgacggaat acagcactaa aggtaagcca aatatcattg
      241 ttttaacaat ggatgattta gggtatggac aattgccttt tgatgatagt tcatttgacc
      301 ctaaaacgat ggataaccga aatgttgttg atacatacaa aataggtatt gataaagcaa
      361 ttgaagctgc aaaaaaatcc actccaacat tagcggccct gatggacgaa ggtgtaaaat
      421 tcactaacgg ctatgttgca cacggtgtct ctggcccttc acgtgccgca atcctgacgg
      481 cgcgagctcc tgctcgcttc ggtgtatatt caaattctga tgcacaggat ggtatccctc
      541 tggaagaaac attcctgtct gaattacttc agaatcatgg ttattacact gcagcaatcg
      601 gtaagtggca cttatcaaaa atcagcaacg taggtgtgcc tgaagaaaaa caaacccgtg
      661 actaccacga taacttcacc accttctccg cagaagaatg gcaacctcag aatcgtggct
      721 tcaactactt catgggttat cacgcatccg gcgtttcact ttataactcc ccgtcattgt
      781 tccgtaaccg tgaaaacatt aaagctgaag ggtacattag tgatcaatta actgatgaag
      841 caattggtgt aattgaccgt gcaagcaaac tggaacagcc atttatgatt tatctggcat
      901 acagtgcacc acacctgcct aacgatcagc ctgcgcctaa aaactatcag gagaaattca
      961 acactggtag caagactgct gataactatt atgcgtcaat ttactccgtt gaccaggggg
     1021 ttaaaaagat cctgaataca cttaaggaaa aaggtcagta cgacaacacc atcattctgt
     1081 tcacctctga taacggtgct gtaattgatg gtccactgcc actgaatggt gcgcaggctg
     1141 gttacaaagg ccaaccgtat ccgggtggta tgcatacacc tatgttcatg tggtggaatg
     1201 gcaagctgaa aacgggtgat tatgacaaac tggtttcatc aatggacttc tatccgaccg
     1261 ctttagatgc cgcagatatc aagatccctg cttctctgaa actagatggt gtatcattat
     1321 tgccatatgt acagaaagga gctgataaga aatctgcagc tggcgaacct cacaaatatc
     1381 tgtcctggat tacctcttat tctcactggt gggacgaaga gaacactgca ttctggaacg
     1441 gatatcataa attcgtgaga aaagagtctg atgattatcc gcacaaccca catacagaag
     1501 acttaagcca gtttgcatac actgtaagaa gcaacgatta ttctctggtt tacacctatg
     1561 cagagaataa actagctctg tacgatatca acgatctgca acaaactaaa gatctctctg
     1621 catcccatcc tgatgtagtt aagaatatga aggctgttat gattgacttc atcacgtcaa
     1681 gtcaaaaacc gatcagtgat atcaaccaag ccaaatttga gaatataaaa aaatctttaa
     1741 ctaacgtgaa atgataataa gcagggagta gatatatcta ctccctggat atagggaaat
     1801 aaatcagtta gagcaataaa taatgaacaa acacgaaaat aaaaaatact gttcatctga
     1861 cgagatttgg tgtacacaac gtaaggagtt aatcaaatct gttcttgaaa caaagaaagc
     1921 ttctgattta gaaaaacaga gtacagatat agctcaacag tatttatcag agacgattca
     1981 gttttctatt gatgatttta tcaaaaatat aaaaagaaaa gttaaatccc gaggttccag
     2041 gcctcccgcc aaacgtaaaa acactcacaa gggaacatag attcaatgcg tttatttaga
     2101 aggtctgtct tctttttaat attaacttat taattggtgc tattcatata attctttaaa
     2161 cagcgaactt atagccagga ttcaatagat atagtaacag gaacatcagt aagcgatgag
     2221 atggtcttcc ccctccttca cgacaacctt taattacgtc atgatggata aactttaacc
     2281 acaaaagtaa gagggatacc atgtctcaac agaaaatcac cggcatcgat ctggctaaaa
     2341 cgaactttta tcttttcagt atcaatgctc atggcaaacc cgcaggaagg atcaaacttt
     2401 ctcgcaatca actgcttaac tggctggtgc agcagcctaa aatgactgtt gcgatggagg
     2461 ctcgcgggca tcccatcact gggccaggga aatcaggaaa ctcgaccatg atgtaatatt
     2521 tcttccggcg caacatgtta aagcttatca gcgacgccag aaaaatgatt atgacgacgc
     2581 tcaggcaata gctgaggcct gccaacatgg aactatacgc cccgtaccaa taaaaactct
     2641 ggagtaacag gacgtccaga cttttttgaa aatgcgccgg ttggtttcga tggaaaggac
     2701 acaactgata aatcacgttc gtggacttct tgctgaatat ggcattgtgt tcagtaaagg
     2761 cgcaacagaa ttacgccaaa aattaccggc attacttgag gatgcggaaa acgaactcac
     2821 tgataccatg aagacgttat tgcatcggca gtatatacgg ttgattacac tagataatga
     2881 actggagtgg tatgattctg aactgaaaaa tatgtccgcc aagatcctat gtgcaaacgc
     2941 ttgttagcca ttcctagatt cagccctctg gttagtcagg caataaaaag ctggatgggg
     3001 gatggaaaac agtttagacg aggaagagat gcatctgcga ctctgggact ggttccccga
     3061 cagttcagca caggtgggaa gcaggttctt ctgggtataa caaaatttgg agaaatgtaa
     3121 tgaacacatc ccaccccatt aagtcaggtg ctctgtgatg gatgaaggga aatactggaa
     3181 ggtgtggagc ctcgcagatt tccggcatca tagtgcccaa tacaggatgc ataaagctcc
     3241 ctcagactgg agactccaca aatgaaatat acaccggttg gcgttgatat cgcaaaacat
     3301 ctgattcagg ttcatttcat cgacgagcac acaggtgaag tgattgataa gcaactacgt
     3361 cgtcaggatt ttctgatgtt cttctgtaat cgggaaccct gcctgattgg tatggaggcc
     3421 tgtagaggct ctcagcactg ggggcgtgaa ctgacaaagc tggggcataa agtccggttg
     3481 ttacaggccc actttgttaa ggcattcgtc atgggaaata agaatgatgt gatggatgcc
     3541 cgggctatct ggatggctgt tcagcagcca ggtaaggaca tcgcggttaa aacagaagaa
     3601 caacagtcag tgctggtttt gcaccgttcc cgcagacaat tggttaagtt ccgcaccatg
     3661 cagattaatt ctctgcacgg cacgctccta gaatttggta aaaccattca caaaggtcgg
     3721 gcagcgatgg agcgagagct ccctgaagca ctggagcgga tgaaagagag actgccgccg
     3781 tatctcatta ctgtgctgga aaaccagtac aaccggctga atgagctgga ctcgctgata
     3841 gaggatattg aaaaaaagct taccagcgtg gcgagacaga atgaaacctg taagcggttg
     3901 ctggatattc ccggcgttgg gccacttatt gcaacggcag cggtggccac catgagggaa
     3961 gcatcagcgt ttaaatcagg gcgagagttc gcggcttatg ttggtctggt gcccaaacag
     4021 actggctcag gagggaaaat ccgcctgctg gagataagca aacgtggtaa tacatatctg
     4081 cgagccttgt ttattcatag cgcaagggcg gcagcattac tgcccaaaga acctggtccg
     4141 tggataaccg aactgaaaaa acgtcgtcca accagcgtgg caatcgtcgc catggcaaac
     4201 aaactagcac gaacagtatg ggcgatagca gctcatggaa gtaaatacga caagaatcat
     4261 gtcagtatca gatcatatta atcgctgata ccgataaata ttaactttta atgaaaggat
     4321 gaatactgaa aggtaactct ggcggccata gtgatgacaa cgacaggtaa gaccgggact
     4381 cactaaacct gaatattgtg ttgagctaaa agctcgctgg agaaatgagg tgtgagtcag
     4441 cgaattacat aggggctcgc agcttcatgg ctgcaaaaaa gccggatata aagctgcaac
     4501 ctacccgtca ctgtcaaggc aatggatgcc ttgcaacggg atgtgttcat ataatcaata
     4561 tgtcagatct atgctgatac agggtgccag agcggtattg ttcagagcct cagagaaaac
     4621 tgattcactt cgtgtgtggg ttaattgtat tcgggaaaag cgtggcttca accaggcagt
     4681 tgttgctctt gcaaataaac tccttcggat cgcctgggca attatcgccc gcaatgagat
     4741 gtatttgccc gcagcaaaac aaaatcaata atctacgtaa aatcagtagg agtaaagctt
     4801 accctgaaat tgcaaagaat gcagaatgat gaaaaa
//
LOCUS       DAZGSP010000205         4829 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZGSP010000205 DAZGSP010000000
VERSION     DAZGSP010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.207"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..374)
                     /locus_tag="ACHK74_005043"
     CDS             complement(<1..374)
                     /locus_tag="ACHK74_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223705.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKTHRAKIVEFD
                     KLDNDSQIDNDFGLHIIYFLQHGHWKVNDRSHQMEKVWFYNSEPSIDIQEYNRFADNT
                     TDTFIFTIIPDNNHVLKLSSPIT"
     gene            757..1809
                     /locus_tag="ACHK74_005044"
     CDS             757..1809
                     /locus_tag="ACHK74_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFP0223706.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(2266..3399)
                     /locus_tag="ACHK74_005045"
                     /pseudo
     CDS             complement(2266..3399)
                     /locus_tag="ACHK74_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558235.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3691..4242
                     /locus_tag="ACHK74_005046"
     CDS             3691..4242
                     /locus_tag="ACHK74_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0223707.1"
                     /translation="MRKYITNSEWALFFNAITGTRNEIRDKALLQITYIHGLRVSELI
                     DLRLGDFDMTEKIVFIRRLKNGLSTSHPLQPETIELLQRWFLIRKRYTKGSQDDYLFL
                     SSHGKKISRQWIYKLCARYSQRAGLRIKVHPHMLRHACGYALANQGLDTRLIQDYLGH
                     RNIHHTVLYTASNPGRFNRVWRS"
     gene            complement(4239..>4829)
                     /locus_tag="ACHK74_005047"
     CDS             complement(4239..>4829)
                     /locus_tag="ACHK74_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP0223708.1"
                     /translation="FSRAFRQQFKVSPVQYRNADAWDMQYAQPVLYRTTPCTKFRMIE
                     IAEGNHPSVPNLKMPVNLGMDFIHTQNGDHFIYNRNLFESIISIYGPVNEMSDFMISG
                     EMIPSRETDSELIYTFKEINSFSLPETLLPDGLYASQEFTGTYKEIFDFHIYDAMGSL
                     CINKCVMKRGPIYTLFHRTDNKNIMDMQFLIPCVKI"
BASE COUNT         1349 a         1024 c          989 g         1467 t
ORIGIN      
        1 gtaatgggag acgataattt taacacatgg ttattgtctg gtataattgt aaagataaac
       61 gtatcagtag tattgtcggc gaatctatta tattcttgaa tatctataga aggttcacta
      121 ttataaaacc atactttctc catctggtga ctacgatcat ttaccttcca gtgaccatgt
      181 tgcaggaaat agatgatatg caggccaaaa tcgttatcta tttgcgaatc attatccagt
      241 ttatcaaatt caactatctt tgcacgatgt gttttatcaa ccttgccatg gtgttgatga
      301 ctgttaactt tatctgaatg cttatcttga tggtttgaag agaagtgctt aaatatattg
      361 tgaaatactt tcataaataa cctgtaatat ttttacttgt tagtaacaaa ataaacaata
      421 catgtctgaa agacatattt acgatgatac aaagcaataa tacttatatg ttcgatgatt
      481 ttaagcaccg agactatccg gtcttttact aaataatgga aaacaatcca tccctggaag
      541 ataattgtaa aaaaacaaca gaataagaat taatcttagg ataaatattt atttatcatg
      601 gccttttaac cggtaaaaaa tggcactcgg cacagtaaac cccgctatag ttatgctatg
      661 catttgtctt taatgatatc tcgtcgttat attaacaggc tgaaattatc attaatgcac
      721 atttcattat tagcagggat acctgaggag tttaaagtga acatatatat cgggtggctt
      781 ttcaaattaa tccctttgat tatgggatta atttgcatcg cgttaggtgg ctttgtgctg
      841 gaaagttcag ggcaaagcga gtatttcgtc gcgggtcatg tgctgatttc tctggcggcc
      901 atatgcctgg cattattcac taccgcattt attatcattt cgcagctcac gcgcggcgtt
      961 aatacgtttt acaatacatt gttccccatt attggctatg cggggtcaat tatcaccatg
     1021 atatggggtt gggcactgtt agcaggcaat gatgtgatgg cagacgagtt tgtcgccggc
     1081 catgttattt tcggcgttgg tatgattgcc gcctgtgtat cgacggtggc agcgtcatcc
     1141 ggtcactttc tgctcattcc caaaaatgca gcggggagca agagcgatgg aacgccggta
     1201 caggcttatt cttcattaat cggtaattgc ctcattgccg ttcccgtttt actcaccctg
     1261 ctcggtttca tttggtctat tacgctgtta cgtagtgctg acataactcc gcattatgtc
     1321 gcgggtcacg tattgcttgg gttaaccgca atctgtgcct gtctaattgg ccttgttgcc
     1381 acaattgtcc atcaaacacg taatacgttt tcaactaaag aacactggct gtggtgttat
     1441 tgggttattt ttctcggctc aatcacggta ctgcagggga tatacgtctt agtcagttcc
     1501 gatgcaagcg cccgactggc tcccggcatt attcttattt gcctcggaat gatctgttac
     1561 agcatattct caaaagtctg gctactggca ctggtatgga gacgtacctg ttcgttagcc
     1621 aacagaatac cgatgattcc cgtcttcacc tgcctgtttt gccttttcct ggcatcgttt
     1681 cttgcggaaa tggcgcagac cgacatggga tattttattc cttcgcgggt tttggtcggt
     1741 ttaggagcgg tatgctttac gttgttctca atcgtttcaa tattagaagc aggttctgct
     1801 aaaaaataat tgcaacgtac cggataaaac cagcgttgac catttgcgta acgctggttt
     1861 ttcttaggca tcatgaaata acgcagcatt aatgcatagt ggttaaggat aaaaaaacaa
     1921 caaagttact gtttttttca acctgttcat attttataaa gatctggcca ctgggtgagt
     1981 tttcaatcac ctttccttcc accttatatt aagcctggag ggtttcagtt cacgggctca
     2041 ttagaaaata atcacaagat agccactatc aaaattagct catttttaat gcggctctaa
     2101 taattttcat ctttaggtaa caggtcagga cgacttcgct gcaattgtgt agaaccaata
     2161 aagttgtatc ctttaagtag aaaagacttt aaagttgcac caattattct atttaactaa
     2221 aaaaacataa agtcaagcac atataacttg tagtggatca caaaatcagg acaccgggat
     2281 aacctgtttc cacttttctt catgtaagca aggcggtaaa ccaccgttgt tcgtgtgagg
     2341 tcgataaacg ttgtaataac cggtttatat ctcgtaccgc atggctaaaa tcaccataac
     2401 cacctttcgg aagccattca cttttaaggc tgcgaaagac tctttccatc agcgaattat
     2461 ccaggcaatt ccctctgcga ctcatacttt gcattacccc ataacgccag agtaactttc
     2521 tgtatttatt gcttttatac tgaacacatt gctctgaatc aaacagcagg cggtcatcac
     2581 gcggtcgtgt ttccagtgca ttacgcaaag ccctgcacac caactcagca tcagcggtta
     2641 atgggagggc tgaaccgata atccgacgtg aatataaatc aacaacgagc gcgagataac
     2701 accatttgtc ctgcaggcga ataaaactga tgtcgccgca ccagaccgcg tctggtgcgg
     2761 cggggttaaa ctgccagttc agtatatttg gcaatggcgg acttttgtct tcgtttactc
     2821 ggtagtgatg tttaaccggc tgtcgacctg tcagcccgca ttcccgcatc agccgtcgtg
     2881 ccagccaccg gcctgcatca acgcaactct ggcgcaacat ctgactgaat gctaggctac
     2941 cggctgcgcc acgactgaga gcatggaaag ccctcacccg acttcgtaat tcaatttttt
     3001 gcacgcttca cctgcgtgta ataaacgcta cggtgaatac cgaataaatg acaaagaacc
     3061 cgcactggcc actttacttt cagccgtgtg attagcgcga cagcttaccg gggattttgc
     3121 ttatcaacac ggcagcctac tttagtgttt ctttttccat ctcagggcgc tttatctgcg
     3181 ctttaagctg ctgaatttcg cgttgttcag gggtaatagc atttcaatac cctgaagttc
     3241 ctgcttatac aaccgtatcc atttacgcaa atggtcaggg ttgagctcga gtgccagcgc
     3301 gacttatctg acatcacgct ggtatttaac cgcctgctcg atagcttcaa gcttgaactc
     3361 cggggaaaag gtacgtttag tccgacgagt tttgatcatg catcacctca ttttcactgt
     3421 tttaacatta acaggatttc gaggtgtcct gaattaccga tccactacac accaacgaac
     3481 gaccatcgtg tgaagaagtt attgccctgg atgtaaactg atttttccac cacgtctcag
     3541 tataggggct tacttattgt ctttgaagac caagcaagat aacgtcaata cggcgttcgg
     3601 gtaacgctta caacacccac ttccgaaact gataagtctt ccttttatct aaaaatgtaa
     3661 aatttacata taactctgac aggaggaaat atgcgtaaat acatcaccaa cagcgaatgg
     3721 gcgctctttt ttaatgctat aacgggtacg cgcaatgaaa tccgtgataa agcattgctg
     3781 caaataactt acattcatgg acttcgcgtc agtgaattga tagaccttag actgggagat
     3841 tttgatatga ctgaaaaaat tgtttttatt aggcggctga aaaacggatt atcaacttca
     3901 catccattgc agccagaaac aatagaacta ttgcaacgct ggttcctgat cagaaaaaga
     3961 tacactaaag gtagtcagga tgattatctt tttctgtcaa gccatgggaa aaaaatttcc
     4021 agacaatgga tttataaact ctgcgcacga tatagtcaac gtgcgggtct gagaattaag
     4081 gtgcatccac acatgttgcg tcacgcctgt ggttatgctc ttgctaatca gggactggat
     4141 actcgtctga ttcaggatta tctgggacac aggaacattc atcatactgt actgtataca
     4201 gccagtaatc ctggtcgttt taatcgggtc tggcgaagtt aaatttttac gcaaggaatg
     4261 aggaattgca tatccataat atttttatta tctgttctgt gaaatagtgt atatataggt
     4321 cctcttttca taacacattt attaatacac agcgacccca tggcatcata tatatgaaaa
     4381 tcaaaaatct ctttgtatgt cccagtaaat tcctggctgg catataaacc atcaggaaga
     4441 agcgtctctg gcagggaaaa actgtttatt tctttaaaag tataaattag ttcagaatcc
     4501 gtttctctgg agggtatcat ttcgcctgaa atcataaagt cagacatttc attaacagga
     4561 ccataaattg atattatgga ctcaaaaagg ttacggttat aaataaaatg atcgccgttc
     4621 tgggtatgga taaagtccat tcccagattc acaggcattt taaggtttgg aacagaagga
     4681 tgattgccct cagctatctc aatcattctg aattttgtgc acggtgtggt tctgtacaat
     4741 acgggctggg catactgcat atcccaggca tccgcattgc gatactgcac tggtgacacc
     4801 ttgaactgtt gcctgaaagc acgggaaaa
//
LOCUS       DAZGSP010000206         4813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZGSP010000206 DAZGSP010000000
VERSION     DAZGSP010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.208"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            38..1153
                     /locus_tag="ACHK74_005048"
                     /pseudo
     CDS             38..1153
                     /locus_tag="ACHK74_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
     gene            1150..1362
                     /locus_tag="ACHK74_005049"
     CDS             1150..1362
                     /locus_tag="ACHK74_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223709.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            1362..2870
                     /locus_tag="ACHK74_005050"
     CDS             1362..2870
                     /locus_tag="ACHK74_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0223710.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLAP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSELTAARIAAALGMYIRKG
                     DGQSYEPDGNGRKENERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRNLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            2815..3957
                     /locus_tag="ACHK74_005051"
                     /pseudo
     CDS             2815..3957
                     /locus_tag="ACHK74_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
     gene            3995..4813
                     /locus_tag="ACHK74_005052"
                     /pseudo
     CDS             3995..4813
                     /locus_tag="ACHK74_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1156 a         1162 c         1423 g         1072 t
ORIGIN      
        1 tttataatca tttagttacc ttttatttac ttcttcttgg atggaagggc cagctgctgc
       61 gtatcagacc tgggcgcaac tggtttacaa attactgact gcagaacagg agtatgaagc
      121 gacaggaagc gaagaaacac tcagggcggt tatcaacacc gactggggat tgccttatct
      181 tcctcgtgcc agcatggagc aacgaaaaag tgaactgctt gagcagcggg cagagccagt
      241 tccttcccgc agtgtgccgg atggcgttaa tttccttgtg gcgacagtgg atgtgcaggc
      301 gggacgtcat cgccgttttg tggttcaggt aacgggctat ggcagtcgtg gcgaacgctg
      361 gattattgat cgttacaaca tcacgcagtc attgcgcagt gactgcgacg gggagagcca
      421 gcgaattgat ccggccagct atccggaaga ctgggatgtc ctgctgacgg atgtttttca
      481 taaaagctgg ccgctggcct ccgatccttc tcaacaaatg cgactgatgg caatggcggt
      541 ggactccggc ggtgaagacg gggtcactga taatgcctat aaattctggc gtcgttgccg
      601 tcgtgatggc cttggtaaac gtatttacct gtttaagggt gacagcatcc ggcgcgcaaa
      661 actgatcagc cgtacattcc ctgataacac cggacgaacg ggccgccggg cgcaggccgc
      721 aggtgatgtg ccgctctggc ttcttcagac ggatgccctg aaagaccggg tgaataacgc
      781 gttatggcgt gactcgccag gtccaggcta tgtgcatttc cctgactggc tggggagctg
      841 gttttacgac gaactgacgt atgaagagcg gagcagtgac gggaaatgga gtaagccggg
      901 tcgcggtgcc aacgaagctt ttgacctgat ggtgtatgcc gaggctctgg tcattctgca
      961 tggatacgaa aagatccgct ggccggatgc accggagtgg gcgagccggg agacctggct
     1021 ggagtgtgtc caggacagta ccgaaccgtc atcctcaccg gaaccggtat ccacgcctgt
     1081 taaaaaacaa aaacggaaga aaacagtaac tgacgatgtt aacccctggc tgacttccgg
     1141 aggatggtta tgaaccagaa tgatatcgaa gccatgattc agcgttatac ggaagctgaa
     1201 atggcggtgc tggacggaaa atccgtcacc tttaatggtc agcagatgac catggaaaac
     1261 ttatctgaga tccggcaggg acggcaggag tgggagcgcc gccttgcggc tctgattaca
     1321 cgacgacggg ggcatcccgg gtaccggctg gcgaggttct gatggcaatc cttgatgatg
     1381 tgattggcgt tttttcacca ggatggaaag cggcaaggct gcgttcccgt gcggtgatcc
     1441 aggcttatga ggccgtaaaa acgacgcgga cacacaaagc ccgacgggag aaccgaactg
     1501 ccgaccagtt aagccagtac ggggccgtgt cgttacgtga gcaggcccgt taccttgata
     1561 acaaccacga tctggttatt ggtgtatttg acaagctgga agaacgggtg gtggggaaaa
     1621 acgggattat tgtcgagcca catccggtat tacgcaatgg ggccattgcc cgtgacctgg
     1681 ctgcggagat tcgcacccga tggagtgaat ggtctgtcag cccggaagtc accgggcagt
     1741 ttacccgtcc gatgctggaa cgtctgatgc ttcgtacctg gctgcgcgat ggtgaggtgt
     1801 ttgcccagat ggtttccggg cgcataaaca gcctggcgcc ttctgccggt gttcatttct
     1861 ggctggaggc gctcgagccg gactttattc ccatgaccag tgatgagagc aacaggctga
     1921 atcagggcgt gtttgttgat gactgggggc gtcccgaaaa atatctggtg tataaaagcc
     1981 gtcccgtatc cggacggcag atggaaacca aagaagtgga tgcagagcga atgctgcatc
     2041 ttaaatttgt tcgccgtctg caccagatgc gcgggacgtc tttgttgtcc ggtgtgctga
     2101 tccgcctcag tgccctgaaa gaatatgaag attctgagct gactgcagca aggatcgccg
     2161 ctgctctggg gatgtacatc cggaaaggcg acgggcagag ctatgaaccg gatggtaatg
     2221 gcagaaagga gaatgaacgt gagcttacca ttcagccggg cattatttac gacgatctga
     2281 aacccggcga agaaatcgga atggtgaagt cggatcgccc caatcctaac cttgaaactt
     2341 ttcgtaatgg tcagttgcgt gccgtggcgg cgggcagtcg tctgagtttt tccagtacag
     2401 cgcgcaacta taacggcact tacagcgccc agcgtcagga gctggttgaa tccactgatg
     2461 gctacctgat cctgcaggac tggtttattg gtgccgtcac ccgcccgatg tatcgtgcat
     2521 ggctgaaaca ggctgtggca tccggtgtta tcaggctacc ccgcgatctt gaccgttctt
     2581 cactgtatac cgcggtgtat tccggaccag tgatgccgtg gattgaccct gttaaggagg
     2641 ctgaggcctg gaaaatccag attcgtggtg gagcggcgac agaatcagac tgggtacgtg
     2701 ctggtggtcg taatccggat gatgtcaaac gtcggcgcaa ggccgaaatt gatgaaaacc
     2761 gcaatctgga tctggtattt gataccgatc cggccagtga taaaggaggc agtagtgccg
     2821 caacgaaacg acaggagccg cagcacaccg acgaccagtc cgaagaataa ttcctggttc
     2881 aggatgcagg ctggtcacca gagtgacgcg gatatttata tttatgacga gattggtttc
     2941 tggggtgtta cagcgaagca gtttatcagt gatctgaatg cactgggcga tatcacccac
     3001 attaatctcc atattaattc accgggtggc gatgtctttg aaggcatcgc catttttaat
     3061 gcgctgaaaa cacatggtgc gtccattacc gtttatgtcg acggtgtggc ggcgtcaatg
     3121 gcgtcggtca ttgcgatggt aggaaacccg gtcattatgc cggaaaacac cttcatgatg
     3181 attcataaac catttggctt tacgggcggt gatgcggagg acatgcgcac ctatgccgac
     3241 ctgctcgata gagttgaggc ggttctgtta cccgcttatg cacagaaaac cgggaaaacc
     3301 accgatgaaa ttgctgccat gctggcggat gagacctgga tgtccggtgc cgaatgtctg
     3361 gcacagggat ttgctgatca ggtgacacca gccgttaagg caatggcatg tattcagtca
     3421 aaacgtacag aggaatttaa aaagatgccg gaatccattc gaaatatgat tactccgcca
     3481 cgcaacagtg ctccacgcgt acaggatgat ggacctgcag cctcccggac gccagtgcag
     3541 gcagcagcac ccgtggtgga tgaaaacagc atccgtgcgc aggtcctggc agagcaaaaa
     3601 gcgcgtgtaa acggtattaa tgatctgttt gccatgtttg gcgggcgtta tcagtcgttg
     3661 caggctcagt gtcttgccga tcctgaatgt tcgctggagc aggcccgcga aaagctgttg
     3721 aacgagatgg ggcgcgagtc cacgccatcc aataaaaata ccccggctca tatttatgcc
     3781 ggtaacggta attttgtggg ggacgggatc cgccaggcgc tgatggcgcg cgccgggttt
     3841 gaaaaaactg aacgtgataa tgtctacaac gggatgaccc tgcgtgaata tgcccgtatg
     3901 tcactgactg aacgtggtat tggtgtttcc agttataacc cgatgcagat ggtcggtgtg
     3961 tagtggcaca ctaaatttgg ccacctgatg aaaggtgata ttctcaccac aacacaaaac
     4021 aggtgactta atgaacaaga aaaccaaacg aaccttcacc cctgagttca ggctggaatg
     4081 tgcacagctg attgttgata agggctactc atatcgacag gccagtgaag cgatgaatgt
     4141 cggttcaacc acgcttgaga gctgggtacg ccagctcagg cgagagcgcc agggtattac
     4201 gccctctgcc acacccatta ctccagacca gcaacgtatc cgcgagctgg aaaagcaagt
     4261 tcgccgtctg gaggaacaaa atacgatatt aaaaaaggct accgcgctct taatgtccga
     4321 ctcgctgaac ggttcacgat agccgccaga ctaagtgaca gccacacggt tgtcagcctg
     4381 tgttctgctc tggaaataca ccgcagcagt taccggtact ggcgaaaacg acgcgatacg
     4441 gttaatccgg cacgagtcag gttgtgcagc gaaatacgcc gggcgtggaa ccaaagtcgg
     4501 ggctctgcgg gcgcgcgcac tctggctgaa atgctgactc aaaacggcgt cccgatgagc
     4561 cgttaccgtg ccggacgtct gatgaaatat ctgaacctga gcagttgtca gcccggaaaa
     4621 catcagtata aaaatgcccg tcaggagcat accagcctgc cgaatctgct tgagcgtcag
     4681 ttcgcagtac cggagccaga ccgggtatgg tgtggagata ttacgtatct ctgggcagga
     4741 aatcgctggt gctatctggc ggttgttatg gatctttttg cccgcagggt tatcggctgg
     4801 agtctgtcag cgc
//
LOCUS       DAZGSP010000207         4740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZGSP010000207 DAZGSP010000000
VERSION     DAZGSP010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.209"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            26..592
                     /locus_tag="ACHK74_005053"
     CDS             26..592
                     /locus_tag="ACHK74_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0223711.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            643..3102
                     /locus_tag="ACHK74_005054"
     CDS             643..3102
                     /locus_tag="ACHK74_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0223712.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGSLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3117..3848
                     /locus_tag="ACHK74_005055"
     CDS             3117..3848
                     /locus_tag="ACHK74_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0223713.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3845..>4740
                     /locus_tag="ACHK74_005056"
     CDS             3845..>4740
                     /locus_tag="ACHK74_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFP0223714.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDE"
BASE COUNT         1300 a         1169 c         1111 g         1160 t
ORIGIN      
        1 ttcttttctg ttaaaggact ccgttatgtt taaaggacaa aaaacattgg ccgcactggc
       61 cgtatctctg ctgttcacag cacctgttta tgcggctgat gaaggatccg gtgaaattca
      121 ctttaaaggt gaagttattg aagcaccgtg tgaaatacat caggatgata ttgataaaga
      181 ggttgaactc ggtcaggtga ccaccagcca cattaatcag tcacatcaca gcgatgccgt
      241 tgctgtcgac ctgcgcttag tcaactgtga tctggaaaac tccagcaacg gttccggtgg
      301 caagatttct aaagttgcag tcacctttga tagctcagcg aaaaccaccg gcgcagatcc
      361 gattctcaac aacaccagca cgggtgaagc caccggcgtc ggcgtacgtt taatgaataa
      421 agatcaaagc aatatcgttt taggcaccgc tactccagat atcgacctgg ctccgacctc
      481 cagcgaacaa acgctgaatt tctttgcctg gatggaacaa attgatcagg ctacacctgt
      541 aacgccaggc gcagttacgg cgaatgcaac ctacgtgctg gattataaat aagtattact
      601 ttggcggcgc tatcgccgcc tttcataaaa tatttatcag gtatggacac cgtgaatatt
      661 tatcgactct cttttgtatc ctgcctggtc gtggcgatgc cttgcgcatt ggcggtcgaa
      721 ttcaacctta atgttctcga taaatcaatg cgcgaccgca ttgatatttc attattaaag
      781 gaaaaaggag tcattgctcc cggtgagtat tttgttagcg ttgcggtaaa taataaccaa
      841 atcagtaacg ggcaaaagat taactggcac aaaaatgacg ataaaaccat tccgtgcatc
      901 aatgatttac tggtcgataa atttggctta aaacctgaag tccgtcagtc gttaccattg
      961 ataaatcagt gcgtcgattt tagctcccga cctgaaatgc tcttcaattt cgatcaagcc
     1021 aatcagcaac taaatatcac cattccgcaa gcctggctgg cgtggcactc agaaaactgg
     1081 accccaccct ccacatggaa agaaggtgtc gccggtgtcc tgatggatta caacttgttt
     1141 gcaagcagct accgcccaca ggatggcagc agcagcacta atcttaacgc ctatggaaca
     1201 gccggaatca acacgggggc atggcgctta cgcagtgatt atcagttgaa tcagactgat
     1261 agcgatgata accatgaaca gtcaggcgaa atatcacgta cctatctttt tcgcccgtta
     1321 ccgcaattag gttcaaaatt aaccctcggc gaaacggatt ttagttccaa tattttcgac
     1381 ggtttttctt ataccggcgc ggcactggca agtgacgatc gaatgttgcc atgggaacta
     1441 cgcggctacg ccccacaaat tagcggtatt gcacagacca atgccacggt gacgatcagt
     1501 caatcaggcc gcgtcattta ccagaaaaaa gtcccaccag gcccatttat cattgacgac
     1561 cttaatcagt ctgttcaggg cacactggat gtcaaagtga cggaagaaga tggtcgggtg
     1621 aacaatttcc aggtttcggc agcatcgacg cccttcctga ctcgtcaggg acaggttcgc
     1681 tataaactgg ccgcgggtca gccacggccc tccatgtcac atcaaactga aaatgaaacc
     1741 ttttttagca atgaagtttc ctgggggatg ctgtcaaaca cctcgctgta cggcagcctg
     1801 ctgctttctg gtgatgacta ccattctgcc gcaataggta tcgggcaaaa tatgctgtgg
     1861 cttggcgcgc tgtcgtttga tgtcacgtgg gccagtagtc actttgatac tcagcaggac
     1921 gagcggggct taagctaccg ttttaattac agcaaacaag tggatgccac caacagcacg
     1981 atttccctcg ccgcttatcg tttctccgat cgtcattttc acagctacgc caactatctg
     2041 gatcacaaat acaacgacag cgatgcgcag gacgaaaaac agacgatcag cttatctgtg
     2101 ggccaaccga ttaccccact aaacctcaat ctttacgcca acctgctaca tcaaacctgg
     2161 tggaatgcag acgcctccac gaccgccaac atcacggcag gttttaatgt tgatattggt
     2221 gactggagag atatctctat ttcgacgtca ttcaatacaa cccattacga agataaagat
     2281 cgcgacaacc agatttacct gtcgatttcg ctccccttcg gtaacggtgg tcgggttggc
     2341 tatgacatgc aaaacagtag ccacagcacc acacaccgca tgtcgtggaa cgatacgctg
     2401 gatgaacgta atagctgggg catgtctgcc ggactgcaat ccgaccgtcc ggacaatgga
     2461 gcccaggtga gcggtaacta tcagcacctg agttcagcgg gtgagtggga tatttctggt
     2521 acctatgccg ccaatgatta cagttccgtc agcagcagct ggagcggttc tttcaccgca
     2581 acccaatatg gtgcagcgtt tcatcgccgc agctccacca atgaaccacg cctgatggtc
     2641 agcaccgatg gcgtggcaga tattccggtt cagggcaatc tcgactacac caaccatttt
     2701 ggcattgcgg tggtgccgtt gatttccagt taccagcctt ccaccgtggc ggtgaacatg
     2761 aatgacttac ccgacggcgt aacagttgca gaaaacgtta tcaaagaaac gtggattgaa
     2821 ggcgcgatag gttacaaatc actggcttcc cgttccggta aagacgttaa cgtcatcatt
     2881 cgcaacgcca gcggtcagtt tcctcccctc ggagcggata tccgccagga tgacagcggc
     2941 attagcgtgg ggatggttgg cgaggaagga catgcctggt taagcggagt cgctgaaaat
     3001 caaaagttta ccgtggtctg gggtgatagc cagcattgct cgctccatct tcctgaacat
     3061 atggaagaca ccgcaaatcg cctgatttta ccttgtcatt aattaaggaa atgaaaatga
     3121 catttatgaa aggactaccc cttttgttgt tggttgccag tctgtgcagc cacgctgcac
     3181 tacaacccga tcgcactcgt attgtgttta acgctaatga taaagctacc agtttgcggg
     3241 tggataatcg tagcgataaa ctcccctacc tcgcttattc ctggctcgaa aatgaaaaag
     3301 gggagaaaag cgatgatctt ctggtcgccc taccgccgat acaacgtctg gaaccgaaag
     3361 caacaacgca ggtacggata gtaaagcaag cctcgaccac aaaattaccg ggcgatcgcg
     3421 aaacgctatt tttctacaat atgcgtgaaa ttcctcccgc cccggaaaaa aacagcgacc
     3481 atgccgttct gcaagtcgcc attcagagtc gtatcaaggt gttctggcga ccggctgcat
     3541 tacgcaagaa agcaggagaa aaggtcgaac tgcagttaca ggtcagccag cagggcaacc
     3601 aactgacact gaaaaatcct accgcgtatt atctgaccat tgcctacctg ggacgaaatg
     3661 aaaaaggcgt tctccccggc ttcaaaaccg tgatggttgc gccttttagt acggtcaaca
     3721 caaacaccgg aaattatagc ggcagccagt tttatctcgg ttatatggat gattacggcg
     3781 cgttacgcat gacgacgctc aactgcagcg gacaatgcca tttacaagcc gtggaggcga
     3841 agaaatgagt gctggcaagg gagtgttgct cgtcatctgt ctgttatttc tgccgttgaa
     3901 gtctgcgctt gccctgaact gctactttgg ttcatccggt ggctcggtag aaaaatcaga
     3961 agcgattcaa ccttttgctg taccaggcaa tgccaaactc ggcgataaga tttgggaatc
     4021 tgacgatatt aaaattcccg tctattgtga caacaatacc aacggcaatt ttgaaagtga
     4081 gcatgtttat gcctgggtaa acccctaccc gggggtgcag gatcgctatt atcaactcgg
     4141 cgtaacttac aatggcgttg attatgacgc caaccaggga aaaagccgca tcgacactaa
     4201 ccagtgtatt gacagtaaga acattgacat ttatacccct gagcagatta tcgcgatggg
     4261 atggcagaac aaaatttgct cgggtgatcc cgctaatatt cacatgtcac gtacctttct
     4321 cgctcgcatg cggttatacg tcaaaatacg agaaatgccg ccgcatgatt atcaaagtac
     4381 gcttagcgac tatatcgtcg tgcaatttga cggtgccggt agcgttaacg aagatcctac
     4441 tgcccaaaac ctgaaatatc atattaccgg tctggaaaac attcgcgtgc tggattgcag
     4501 cgtcaatttt tccatttcac cggaaacaca ggtgattgat tttggtaaat ttaatttgct
     4561 ggatattcgt cgccacacaa tgtcgaaaac attcagtatt aagacgacaa aatcacaaaa
     4621 tgaccaatgc accgacggat ttaaagtcag ttcctccttt tataccgaag aaacgttggt
     4681 tgaagaagat aaagcattgt tgattggtaa tggtctgaag ctacgtttgc tggatgaaaa
//
LOCUS       DAZGSP010000208         4700 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZGSP010000208 DAZGSP010000000
VERSION     DAZGSP010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.210"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..80
                     /locus_tag="ACHK74_005057"
     CDS             <1..80
                     /locus_tag="ACHK74_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP0223715.1"
                     /translation="IGFANTTFSDADFGALGILLGIIVR"
     gene            complement(296..580)
                     /locus_tag="ACHK74_005058"
     CDS             complement(296..580)
                     /locus_tag="ACHK74_005058"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0223716.1"
                     /translation="MEQKTLSAEPRRSFSNEFKLQVVKLALQPRVSVARIAREHDIND
                     NLLFKWLRLWLIKGRISRRLPVRNSSGIGVELLPVEMTLDERPYPVFYAR"
     gene            complement(711..3392)
                     /locus_tag="ACHK74_005059"
                     /pseudo
     CDS             complement(711..3392)
                     /locus_tag="ACHK74_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
     gene            complement(4066..>4700)
                     /locus_tag="ACHK74_005060"
     CDS             complement(4066..>4700)
                     /locus_tag="ACHK74_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFP0223717.1"
                     /translation="VSVVIPGWQFLTQPSPAEQQLRALLAPLPDVLSADQMAQLSRTP
                     SLLNHASEWITLSGQQITRLTELPPAYNLQRSAQLLQQLRGLFPDNPRVQEMVDNWQK
                     SVRSRALPEEAMTGWNEGMTRLQQLAERLNRLDEQRGKYMTVSELKTEVFGIMQAFNR
                     HIPAEEQLRRYDEARNQNGSEQQQKQAEMALNQLINRYQMEHTGNQERQP"
BASE COUNT         1268 a         1130 c          925 g         1377 t
ORIGIN      
        1 acattggttt tgctaacacc accttcagtg atgctgactt tggcgcactg ggtattttgt
       61 tagggattat cgttcgctaa tattttgatg acattaatat cctccaggat ttcaggaacg
      121 tggaggatat taattcctct tattttctcc atttttcttt cttccagtca ggactgcatc
      181 tgattaatcg taagcgctcc gggaagtacg tcgcgtaagg attattttac agctgagaag
      241 tttcagtgca gcagttcatg cacacggttc gacggctagt cattgatctt cataattacc
      301 tcgcgtaaaa aacggggtaa ggccgctcat ccagcgtcat ctccacaggt aataattcaa
      361 cgccaatgcc agaagagttc cttaccggaa gacgccgtga tatacgccct ttgatcagcc
      421 agagcctgag ccatttgaac agaagattat cattgatatc atgctcccgg gcaatacggg
      481 caacagaaac ccttggttgt aaagccagtt taaccacttg aagtttaaac tcatttgaaa
      541 atgacctgcg gggttcagct gataatgttt tttgttccat aacaggcatc cactagtttg
      601 aaaaggtggg cacctacgtt accaatactg gcttaatggc tacatacgac ggtcggttta
      661 cgcttaccag aaaactaata gtgctatatg caatttttaa tattcagact ttagcctgac
      721 aggcctgaca aaaacgcgcc caacttccct tctatatcac tgtctatagt acgtaatctt
      781 agcaatggca gaccacattt cttcagtata ctgtttttaa gttcatcacg ttcagcctag
      841 acactggtca gatgatagcc tccatccact tcaataaccc ctaatggagt cttcccgacc
      901 ttatagtaaa caacaaagtc acaactggcc cgattacgca tgaataacag ttcacggtga
      961 gtaaaatctc cccgctccag caacaccaac tgattcagcg ctatttgtga atgaaacatc
     1021 atgctccgat aactatcctg cgacaggata tcccgcaaca ggcaggccac aatctgttct
     1081 gacttaaagc gtgaatcatc tgaattcaat ctactattca gtcgttccag tgatttgtca
     1141 tactcactgt acagcaaatc aaatgctgat atcaccgggc tctcgaaaat ctctccgtca
     1201 tcagcgtagt atctgatgta acgaatcagt gccgcaatat gtccagcgtg gcgttcaaat
     1261 acatcattac cagtcaccaa cgtaaatttg tttcgggccc ttgaaacagc gacattcacc
     1321 agttcaggat tatcaacaaa cgcgatattc cgactttgct ggctgctgcg tttcttatcc
     1381 agcacagtgg aaaacacaat ttcatcgcac tcccgtccct gaaatttatg caccgtcgat
     1441 ttcacaaaat cagcgggcag cactgtttca gccagattaa cctgtgcatt atatggggcg
     1501 atatatcccc tgctactttc ctcatcccag taatgtcctt ccagagattc aagctccctg
     1561 agatttgaaa aattacgggt atggtttcct tttgccgtga tgatcagaga taatgaggct
     1621 tcacctgaat caagcgttag cggtatcaga gcattatcat aaaactgctt attacaaaat
     1681 tgaatgattt taggatgaca gcgataatgc tctttaagaa gagttactgg caccatattc
     1741 ctgaacaaca tacaaaccga atcaagtaga ctatatttct cacaattata gtattctgcc
     1801 ggtggagatg ggctgttcgg gagtaataca ggaacatgtg gaagttgctt tcgatcccct
     1861 acaacgatta cattacgggc acatcctagt cccagaatgc ccggaacaat atcctgctgt
     1921 gaggcttcat caataatcac ataatcaaga agagccccct taccgataga gttaataata
     1981 gaatgcgtgc tgctaccaat aaccggaaaa cgctttataa aacggttaaa ttcatccctg
     2041 tatgtctctg ccgagaatga atttcttgtt gaaatatttc ttcgcagatg ttgttttaaa
     2101 taaagcacag accatgatgt caggcgctcc agtaaaattt taaaatcaga ttcctttaat
     2161 actttctcat attcattcaa agaaatttgt ttttcccgga tggatttttc ataataagaa
     2221 agctgaagag aagtaaatag tgccagcctt ctctccggaa tcaccaatgg tttgactttc
     2281 agtatcctga agtttaacaa caaatcaatt ctgtttctga atccaatccg cctgtcagaa
     2341 aggtaatgaa tataagccat caggtcagtt gtttttcgtg aggaaagtcg gtatttgtga
     2401 gaaaaaagct cctccggtcg aagattctga ctctgccatt gctgcaggta tttatactca
     2461 acactcagtt cattaatttc tgcttttaac gaagcaacct ggttgatagc attcaggtgt
     2521 tttttcactt gttgaagtac atcatcaatc atatttgcag acggtgagtc aggtaagact
     2581 tcctcagaac gcgaaatcga cgttgaaaaa aactgttgtc tgttctccgt acttcccagt
     2641 cgtgccacca catatcccag tcgttgtttg tccatttttt cataaacatt actgacagct
     2701 gagttattat tggacaaaat tgccactgtt ttattctgaa ttaatatatt ggcaacaata
     2761 ttgagaatag tctgtgtttt acctgtccca ggtggtcctt caataactga aatctgtgag
     2821 gaaaatgccc gttcaaccgc cattaactga ctctcattca ggccaaacgg aaaaatcaac
     2881 ccccatggcg aatcacgttt tttactctgt cctgaacagt atgcatgcaa ggcggtatcc
     2941 ggatgaggaa ctattttctt tatctgtctg aggatattct catcgatatt tttattatta
     3001 cctcgagcat aaaaaacacg ttcgtcagcc accctgcaca agtaattaaa cacggcatgc
     3061 tctgtaaatg ctgaacactg ttgaaagtca agtccggtcg ttttcatgat gtagggtcgt
     3121 tcgctctcac ggtaataaat agcagcatat ttatcaccat agataataac ccgttcagcc
     3181 cgggatgtta cagcgttccc tttcctttcg aggaaaacat ttcgcaaaat tacagttggc
     3241 gttatctgac atacagaaag cggacgagta tattttttcc cgctgcgaaa atgacaggtc
     3301 agcataacct ctccgtcatc atttccagcc cacagagtcc agtcggtaat tttatgagtc
     3361 ttattttcac cgtcaacaca aatcgtgacc atattctcac tcagtaatta tcatttaact
     3421 tacggaaaat tgttatgaaa ctcccgatat ttacccggta aaatacttgt cattgtttga
     3481 acccctccct aaaaaatgac caggcatttg ataacccggt gtatttctcc cgatatgagc
     3541 tccggaggat tttccacaac tccgggtgtg ttaacatatt cccgccggcc atatatacat
     3601 tgagttcagt cataacagtc aataccagga ttacaggaac gcgatgctga tacgctggtt
     3661 attgaatact ggtagcccct aactactaat tcatcacgat actctgtact tactgttttt
     3721 tacaaccatt cccccaggta aaagagtcca tttatcatgc ccctctttaa cgcctgccag
     3781 cataatttaa gaatggatac cgtgggtgga attaacaata cctgctcagg ccaatgccat
     3841 gtaaccgcct cactgaaaaa gccagatcat ctaaccaggt acagaccaac atcgcaaaaa
     3901 ttaccatgta gcacgaactg ctgtcatatt tctgttcagg tatgccgcta tttcgcgggt
     3961 tttcttcacg ccataatgcg tttcaaggta gcgaacatct tcgattgtcc atggtgtgcg
     4021 cgctcttccg ggggtatttt ccatttccat gttttatctc agccattatg gctgcctctc
     4081 ctgattgcct gtgtgttcca tctgataacg attaatcagc tgattcagtg ccatttcagc
     4141 ctgtttttgc tgctgttcac tgccattctg attacgggct tcatcatagc gacgtaactg
     4201 ctcttctgcc gggatatgcc ggttaaaagc ctgcatgatg ccaaacacct ccgttttcag
     4261 ttcactgacc gtcatgtatt ttccccgctg ttcatccaga cggttcaggc gctcagccag
     4321 ctgctgtaag cgggtcatgc cttcgttcca gcccgtcatc gcctcttccg gaagcgctcg
     4381 gctccttacg ctcttctgcc agttatccac catttcctga acacggggat tgtcggggaa
     4441 aagtcccctc agttgctgca gcagctgcgc actgcgttgc aggttgtatg ccggcggtag
     4501 ttctgtcagt cgcgttatct gctgaccgga aagggttatc cactctgacg catgatttaa
     4561 cagtgagggt gttctgctga gttgcgccat ctggtctgct gaaagcacat ccggtagtgg
     4621 tgccagcagc gccctgagtt gctgctccgc cggtgatggc tgtgtcagaa actgccatcc
     4681 cggaatgacc acgctgacag
//
LOCUS       DAZGSP010000209         4615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZGSP010000209 DAZGSP010000000
VERSION     DAZGSP010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.211"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..439)
                     /locus_tag="ACHK74_005061"
     CDS             complement(<1..439)
                     /locus_tag="ACHK74_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP0223718.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEEL"
     gene            complement(554..629)
                     /locus_tag="ACHK74_005062"
     tRNA            complement(554..629)
                     /locus_tag="ACHK74_005062"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(594..596),aa:Thr,seq:ggt)
     gene            complement(636..710)
                     /locus_tag="ACHK74_005063"
     tRNA            complement(636..710)
                     /locus_tag="ACHK74_005063"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(675..677),aa:Gly,seq:tcc)
     gene            complement(827..911)
                     /locus_tag="ACHK74_005064"
     tRNA            complement(827..911)
                     /locus_tag="ACHK74_005064"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(875..877),aa:Tyr,seq:gta)
     gene            complement(920..995)
                     /locus_tag="ACHK74_005065"
     tRNA            complement(920..995)
                     /locus_tag="ACHK74_005065"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(960..962),aa:Thr,seq:tgt)
     gene            1357..2307
                     /gene="coaA"
                     /locus_tag="ACHK74_005066"
     CDS             1357..2307
                     /gene="coaA"
                     /locus_tag="ACHK74_005066"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP0223719.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2336..3301)
                     /gene="birA"
                     /locus_tag="ACHK74_005067"
     CDS             complement(2336..3301)
                     /gene="birA"
                     /locus_tag="ACHK74_005067"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFP0223720.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3298..4326)
                     /gene="murB"
                     /locus_tag="ACHK74_005068"
     CDS             complement(3298..4326)
                     /gene="murB"
                     /locus_tag="ACHK74_005068"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFP0223721.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1169 a         1166 c         1051 g         1229 t
ORIGIN      
        1 gcagctcttc gtcatcaacc atgtcgcatt tgttcaggaa cacgatgatg tacggaacgc
       61 ctacctgacg acccagcagg atgtgctcac gagtctgcgg catcgggccg tcagtcgcag
      121 caactaccag gatcgcgccg tccatctgag cagcaccggt gatcatgttt ttaacatagt
      181 cggcgtgccc cgggcagtct acgtgtgcgt agtgacgggt cggggtgtcg tattcaacgt
      241 gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg atctggtcga
      301 atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca gcggtcagcg
      361 ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt tttgtacgtt
      421 caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg tgatatcacc
      481 acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga aaaaacaggg
      541 aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc cttaccaagg
      601 gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg gaatcgaacc
      661 cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc atcctggaac
      721 tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt attcgagccg
      781 gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt ggtgggggaa
      841 ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt tggccgctcg
      901 ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac ctactgatta
      961 caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca ttctatggag
     1021 acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca cattttatgc
     1081 gacacgatga agaaacagcc gaacggaggg cagattagca cactttttca acatcattgt
     1141 gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat atctgtcaca
     1201 ttgcttttca acgatagctt cctggcagag attttttctt attattcctc cccatctggt
     1261 gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg caaatatcgc
     1321 tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga gcaaacgtta
     1381 atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga ttccgtacct
     1441 atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga tctctcgtta
     1501 gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta tataagctcg
     1561 aatctgcgcc gtcaggcagt tctggaacag tttcttggta ctaacgggca acgcattcct
     1621 tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc ccgtgtattg
     1681 caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac tacagatggc
     1741 ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa aggcttcccg
     1801 gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc tggcgtgcca
     1861 aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga tggagataaa
     1921 acggttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca gagcgggatg
     1981 gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt ttcgatatat
     2041 gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct gaaattccgc
     2101 gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt aactaaagaa
     2161 gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa cttaaagcaa
     2221 aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc taatcatgcg
     2281 gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc ctttcttatt
     2341 tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt ccatcctgct
     2401 caagtaataa agccccctgt ttatctattc cgcgtgaaat gccaaatatt tctttatcac
     2461 caatgataag tttcactggg cgattaataa aattatccag cttttcccag cgcgacagat
     2521 aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca cgtattagca
     2581 tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg atccacccct
     2641 gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct ccaatgacta
     2701 tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc agcttgcgat
     2761 cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc agcttgcgta
     2821 atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc gccgggcctt
     2881 gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa aaccatttcc
     2941 gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg cccgatttaa
     3001 gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc agcacggcta
     3061 cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg atgggctcag
     3121 gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca cgcagtgtct
     3181 gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac tgctcgccag
     3241 agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg ttatccttca
     3301 tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga cttcaggctc
     3361 aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca gttgcaccac
     3421 atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac ggtgcaccgc
     3481 agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac ctgctgccag
     3541 ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg ccagtaatgc
     3601 tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat tgccattcac
     3661 ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata cttgctgtgg
     3721 cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag gttgccactc
     3781 ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt catgtttaaa
     3841 aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt gcttgcctgt
     3901 cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc cataagcacc
     3961 aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat tttccagacc
     4021 aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc cggctcctac
     4081 atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc ggttgatgat
     4141 caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca gaataagaac
     4201 gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt cgtcttcggc
     4261 acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt ttaaggagtg
     4321 gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg tggggggatg
     4381 gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat
     4441 ggccttctgc ttaatttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc
     4501 gggccgttgc ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
     4561 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tttgactgag ccttt
//
LOCUS       DAZGSP010000210         4361 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZGSP010000210 DAZGSP010000000
VERSION     DAZGSP010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.212"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            276..1208
                     /gene="rfaD"
                     /locus_tag="ACHK74_005069"
     CDS             276..1208
                     /gene="rfaD"
                     /locus_tag="ACHK74_005069"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFP0223722.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1218..2264
                     /gene="rfaF"
                     /locus_tag="ACHK74_005070"
     CDS             1218..2264
                     /gene="rfaF"
                     /locus_tag="ACHK74_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFP0223723.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLYKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2268..3260
                     /gene="rfaC"
                     /locus_tag="ACHK74_005071"
     CDS             2268..3260
                     /gene="rfaC"
                     /locus_tag="ACHK74_005071"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFP0223724.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3257..>4361
                     /gene="rfaL"
                     /locus_tag="ACHK74_005072"
     CDS             3257..>4361
                     /gene="rfaL"
                     /locus_tag="ACHK74_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFP0223725.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTTKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIIL"
BASE COUNT         1161 a          978 c         1054 g         1168 t
ORIGIN      
        1 gaattatatc ataaatatta acagaatgta tactgtatat tatccattca aatgcttccg
       61 ttatttatca tacatcggaa tattgatact aaagcactat tcacatgcaa aaccaacatc
      121 cgccatgaag gactagctaa aacccaaact agtttgttgc aattagcatc cttgcacctc
      181 tatgtaaagg gctgaaggga ttcggatgtg atggtatgat tacagacatt cgtgtctgag
      241 attgtctctg actccataat tcgaaggtta cagttatgat catcgttacc ggcggcgcgg
      301 gctttatcgg cagcaacatc gttaaggccc tgaatgataa aggcatcacc gatattctgg
      361 tggtggacaa cctgaaagac ggcaccaagt ttgtgaacct ggtggatctg gatatcgcgg
      421 actatatgga taaggaagac ttcctgatcc agattatggc tggcgaagag ttcggcgatg
      481 tcgaagcgat tttccacgaa ggcgcatgct cttccaccac cgagtgggat ggcaagtata
      541 tgatggataa caactatcaa tactccaaag agctgctgca ctactgtctg gagcgcgaaa
      601 tcccgttcct gtatgcatct tccgcggcca cctacggcgg acgcacctcc gactttattg
      661 aatcccgcga gtacgaaaaa ccgttgaacg tctacggtta ctcaaaattc ctgtttgatg
      721 aatatgttcg tcaaatcctg cctgaagcga actcgcagat tgttggcttc cgttatttca
      781 acgtttatgg accgcgtgaa ggccataaag gcagcatggc gagcgtcgct ttccatctca
      841 acacccagct taacaacggt gaatcaccga agctgtttga aggtagcgag aacttcaaac
      901 gcgacttcgt ctatgtgggc gacgtggctg atgtgaatct gtggttcctg gaaaatggcg
      961 tttccggcat cttcaatctc ggtactggtc gtgcggaatc cttccaggct gtagctgatg
     1021 ctacgctggc ttatcacaag aaaggccaga tcgaatacat tccgttcccg gataaactga
     1081 aaggccgcta tcaggcgttc actcaggcag atctgacgaa tctgcgcgcg gcgggttacg
     1141 acaaaccgtt caaaaccgtt gctgaaggtg taacggaata catggcctgg ctgaatcgcg
     1201 acgcataaga gctctgcatg aaaatactgg tgatcggccc gtcttgggtt ggcgacatga
     1261 tgatgtcgca aagtctctat cgcacgctcc aggcgcgcta tccccaggcg ataatcgatg
     1321 tgatggcacc ggcatggtgc cgtccattat tatcgcggat gccggaagtt aacgaagcta
     1381 ttcctatgcc tctcggtcac ggagcgctgg aaatcggcga acgccgcaaa ctgggtcata
     1441 gcctgcgtga aaagcgctac gaccgcgcct acgtcttacc caactccttc aaatctgcat
     1501 tagtgccttt cttcgcgggt attcctcatc gcaccggctg gcgcggcgag atgcgctacg
     1561 gtttactcaa cgatgtacgc gtgctctata aagaagcctg gccgctaatg gtggaacgct
     1621 atatagcgct ggcctatgac aaaggcatta tgcgcacagc acaagatctg ccgcagccat
     1681 tgttatggcc gcagttgcag gtgagcgaag gtgaaaaatc atatacctgt aatcaatttt
     1741 cgctttcatc agaacgtccg atgattggtt tttgcccggg tgcggagttt ggtccggcaa
     1801 aacgctggcc acactaccac tatgcggagc tggcaaagca gctgattgat gaaggttatc
     1861 aggtggttct gtttggctcg gcgaaagatc atgaagcagg caatgagatt cttgccgctt
     1921 taaataccga gcagcaggca tggtgtcgga acctggcggg ggaaacacag cttgatcaag
     1981 cggttatcct gattgcagcc tgtaaagcca ttgtcactaa cgattctggc ctgatgcacg
     2041 ttgcggcggc gctcaatcgt ccgctggttg ccctgtatgg tccaagtagc ccggacttca
     2101 caccgccgct atcccataaa gcgcgcgtga tccgtctgat taccggctat cacaaagtgc
     2161 gtaaaggtga cgctgcggag ggttatcacc agagcttgat cgacattact ccccagcgcg
     2221 tactggaaga actcaacgcg ctattgttac aagaggaagc ctgacggatg cgggttttga
     2281 tcgttaaaac atcgtcgatg ggcgatgttc tccatacgtt gcccgcactc actgatgccc
     2341 agcaggcaat cccagggatt aagtttgact gggtggtgga agaagggttc gcacagattc
     2401 cttcctggca cgctgccgtt gagcgagtta ttcctgtggc aatacgtcgc tggcgtaaag
     2461 cctggttctc ggcccccata aaagctgaac gcaaagcgtt tcgtgaagcg ctacaagcag
     2521 agaactatga cgcagttatc gacgctcagg ggctggtaaa aagcgcggca ctggtgacac
     2581 gtctggcgca tggcgtaaag catggattgg actggcaaac cgctcgcgaa cctttagcca
     2641 gcctgtttta caattgtaag catcatattg caaaacagca gcacgccgta gaacgcaccc
     2701 gcgaactgtt tgccaaaagt ttgggctata gcaaaccgca aacccagggc gattatgcta
     2761 tcgcacagca ttttctgacg aacctgccta cagatgctgg cgaatatgcc gtatttcttc
     2821 atgcgacgac ccgtgatgat aaacactggc cggaagaaca ctggcgagaa ttgattggtt
     2881 tactggctga ttcaggaata cggattaaac ttccgtgggg cgcgccgcat gaggaagaac
     2941 gggcgaaacg actggcggaa ggatttgctt atgttgaagt attgccgaag atgagtctgg
     3001 aaggcgttgc ccgcgtgctg gccggggcta aatttgtagt gtcggtggat acggggttaa
     3061 gccatttaac ggcggcactg gatagaccca atatcacggt ttatggacca accgatccgg
     3121 gattaattgg tgggtatggg aagaatcaag tagagtgtcg ttctacaagt atgtctcttg
     3181 cagatttgcc agctcaaacc gtttttcaaa atttaaatct tgaaataata accaataagt
     3241 tgacatcgga gataagatga cctcaacatt atttttctct ctcgagaaaa aaaactggat
     3301 agcgtactgg aacagagctc tcgtattctt attcattacc acctattttt tgggtgggat
     3361 tacaaggtat aaacatctta ttgttattct tatgacaata acgacaatcg tctatctctg
     3421 caaacggcca aaacactatc tctcactatt taaaacattt ctttttggta gtgttgccat
     3481 attaactatt gctgcattgc tgtcacttct tcaatcccct gatgcaggtg ctagcatgaa
     3541 ggaaattttc aaagctatta ttgagaatac tttactatgc acaatagcaa taccgatcat
     3601 attgagagac gagaaaagag aagatgtcga aaaaatcgtt ttcttctcat ttattagtgc
     3661 gttgggctta cgctgttttt ctgaattgat tacctattat aaggactatc aacaagggat
     3721 aatgccattc gcagattata gacaccgtag catttctgac tcgatggtct ttttattccc
     3781 tgcattgtta aatctctggc ttatcaaatc agcaaaatac cgcatttctt ttgtggttct
     3841 aagcgttatt tttatttttc tgatattagg aactttatcc agaggggcct ggctttccgt
     3901 gttagtcatt ggattaatat ggattctgat gtttaaacaa tggaagttac tattagtagg
     3961 agtaatggtt gccatcattg cattgtcggt tattttcaca cataaggaga tgaccacaaa
     4021 gctaacgtat aaacttcaac aaactaatag ttcttatcgc tatgcaaatg gtactcaagg
     4081 cagcgcactc gatctaatat tagaaaatcc tgttattggt tatggttacg gtaacgttgc
     4141 atataaagat gtctataata aacgtgtcat tgattatcca gaatggacct ttagacaatc
     4201 aatagggcca cataattttg cgctattcat ctggtttggc actggtttat tagggctggt
     4261 aagtcttatg atgctatact gtgcaatatt gaaagagtgt ataaaaaatg gcgtcaagaa
     4321 taaatatcgc tcaccatata atgcatatta tataatctta c
//
LOCUS       DAZGSP010000211         4322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZGSP010000211 DAZGSP010000000
VERSION     DAZGSP010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.213"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            51..1004
                     /gene="ompT"
                     /locus_tag="ACHK74_005073"
     CDS             51..1004
                     /gene="ompT"
                     /locus_tag="ACHK74_005073"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFP0223726.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1191..2675
                     /locus_tag="ACHK74_005074"
     CDS             1191..2675
                     /locus_tag="ACHK74_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0223727.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
     gene            complement(2858..3070)
                     /locus_tag="ACHK74_005075"
                     /pseudo
     CDS             complement(2858..3070)
                     /locus_tag="ACHK74_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            3220..3888
                     /locus_tag="ACHK74_005076"
     CDS             3220..3888
                     /locus_tag="ACHK74_005076"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP0223728.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(3833..3970)
                     /locus_tag="ACHK74_005077"
     CDS             complement(3833..3970)
                     /locus_tag="ACHK74_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFP0223729.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(3943..>4322)
                     /locus_tag="ACHK74_005078"
     CDS             complement(3943..>4322)
                     /locus_tag="ACHK74_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0223730.1"
                     /translation="DHRGKTVYDVASGDALFISELGPLPENVTWLSPEGEFQKWNGTA
                     WVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEETSLLEAWKKYR
                     VLLNRVDTSTAPDIEWPTNPVRE"
BASE COUNT         1322 a          956 c          861 g         1183 t
ORIGIN      
        1 atataaaaaa tacatattca atcattaaaa cgattgaatg gagaactttt atgcgggcga
       61 aacttctggg aatagtcctg acaaccccta ttgcgatcag ctcttttgct tctaccgaga
      121 ctatatcgtt tactcctgac aacataaatg cggacattag tcttggaact ctgagcggaa
      181 aaacaaaaga gcgcgtttat ctagctgaag aaggaggccg aaaagtcagt caactcgact
      241 ggaaattcaa taacgctgca attattaaag gtgcaattaa ttgggatttg atgccccaga
      301 tatctattgg ggctgcgggc tggactactc tcggcagccg aggtggcaat atggtcgatc
      361 aggactggat ggattccagt aaccccggaa cctggacaga tgaaagtagg caccctgata
      421 cacaactcaa ttatgccaac gaatttgatc tgaatatcaa aggctggctc ctcaacgaac
      481 ccaattaccg cctgggactc atggccggat atcaggaaag ccgttatagc tttacagcca
      541 gaggtggttc ctatatctac agttctgagg agggattcag agatgatatc ggctccttcc
      601 cgaatggaga aagagcaatc ggctacaaac aacgttttaa aatgccctac attggcttga
      661 ctggaagtta tcgttatgaa gattttgagc taggtggcac atttaaatac agcggctggg
      721 tggaagcatc tgataacgat gagcactatg acccaggaaa aagaatcact tatcgcagta
      781 aagtcaaaga ccaaaattac tattctgttg cagtcaatgc aggttattac gtaacaccta
      841 acgcaaaagt ttatgttgaa ggcgcatgga atcgggttac gaataaaaaa ggtaatactt
      901 cactttatga tcacaatgat aacacttcag actacagcaa aaatggtgca ggcatagaaa
      961 actataactt catcactact gctggtctta agtacacctt ttaacgaagt taaccagatt
     1021 ttctccccgg tggtattgta taaccccggg gaatttgttt acagttacaa gtagacaata
     1081 tccactaatt ttgtaagcat ttaacctatg tcataacgtg taatctctta ccgcaacaca
     1141 agacctcaat atattcaaat cttatcttta ttccgatatt cagagacaca atgcgaaaaa
     1201 taattactca tttcaaagtt gttttaacgt tacttctacc agtaaccgta tctgcccagc
     1261 agatacagtg gcaatcctgt atggccagtc aattcaacca ctggtttggt gaggaaaaac
     1321 cgtctcctga cttactatgt ggttatttgt ctgttccatt aaaatataca gacacaggcg
     1381 gagatgcttc ttatgaaaaa aaatcacaag tcaaactagc gttgacaaaa ttgccggcaa
     1441 aaagcaagca taaaggaagt atcctgataa taagtggtgg tcccgggtta ccaggcataa
     1501 atccttatat taactttgac tggccagtca caaatcttcg tgagtcatgg gatattattg
     1561 gatttgatcc tcgaggcgtc ggacagtcca ctccgacaat aaactgccgg caatcagata
     1621 cagagactca ggaaaacata actgaaaagc aacaagtatt aaataaaatt aatgcctgta
     1681 tccataatac cggagccgaa gtcattcgcc atatcggctc taacgaggct gtatacgata
     1741 ttgatcgtat taggcaagcc ttgggggata aacaactgac agccgtggcg tattcgtatg
     1801 gaactcaaat tgcagcctta tatgcagaac gttttcccta caacgtaaga tctatcgttc
     1861 ttgatggagt cgtcgatatc gatgacctgg aggacaactt cacatggcaa ctcaaacagg
     1921 cacagagtta tcaggaaacg tttgatcgct ttgcatcctg gtgtgcgcgt acaaaaagtt
     1981 gcccgctttc ttcagacaga gataaggcaa taactcagtt ccatgagcta ttatcaaaat
     2041 tacatcacaa acctttatta gacagtaagg gagaaaatat atcttcagat gaactcatat
     2101 cattaacaac agaccttctg ctatggcgtt catcatggcc aacccttgca actgccatac
     2161 gccagttctc tcaggggatt gtcagtaatg aaattgaaac tgcgctcagt gctccgatag
     2221 cctcagaaga gtcaagcgat gcttcggggg taatcctctg tgtagatcag ggggatgagc
     2281 aattaacacc agaagagcga aaatcccgaa aagacgctct tgcgaatgcc ttcccggcta
     2341 ttaactttga caatggacgt tccgattcac ctgatttttg tgaattatgg ccaatacata
     2401 gcgacctgaa caaaactcgc ctgaaaaata ctgttctgcc ctctggttta ctgtttgtag
     2461 cacacaaata cgacccaaca acgccctgga ttaatgcccg taagatggca gagaaatttt
     2521 ccagcccgtt actaacaata aatggtgatg ggcatacatt agctctcacc ggagttaatt
     2581 tatgtgtaga taaagcagtt gtacatcacc tgatcactcc acaaaaaata gaaaatatat
     2641 actgcccagg aaattctgaa gcagaaatac aataatttca aaatattccg ctatttgctc
     2701 ccaatgcaaa acatatattg cattgggagc atattcatat ttttgttatt cttgtatgaa
     2761 atcgtctcat gtacacggtc ctttttcgcc gaaaactgat attactttaa tcaaaagttg
     2821 acgtgccaga ttcgaagtaa atttataaaa tatcaaatca atatacattt tgcaccttcg
     2881 agatagtaat ggctgttact gtaacatgag ttacaaacag cttcataccc agagctactg
     2941 atgtatcaac acgattcaac aatacccgat atttattcca tgcctccagc aacgatcttt
     3001 cttcctccgt tgcgatttcc agatctacag catcctgcag tggcgcaata tactcactga
     3061 attcctggat gtagaactgt gtggtgacgg tcttccagcc attcggctcc tgctgtatcg
     3121 aagcatacca ggctatttca atatcgctat gctgcggcag catttaaccc cttgtaattc
     3181 atcgccataa ttgatttaat tcacaaataa aactataaca tggtgaaatt aatgaaaaaa
     3241 aacacagatg atggggctaa aatttacaca ccacttaccc taaagcttta tgactggtgg
     3301 gttttgggag tatcaaatcg gcttgcatgg ggatgtccta caaaggaaca ccttcttcca
     3361 cactttctgg aacatttagg taacaaccat ctggatattg gtgttggaac tgggttttac
     3421 cttactcacg tacctgagag tagtctgata tctttaatgg atttgaacga agctagcctg
     3481 aacgcggcat ctacaagggc tggggaatca aaaattaaac ataaaattag ccatgatgtt
     3541 tttgaacctt atcccgcggc gttacatggt caatttgatt ccatttccat gttttacctt
     3601 cttcactgcc tgcctggaaa tatatctaca aaaagctgtg taatacgcaa tgcggcgcag
     3661 gccttaactg acgatggaac tctatacgga gccacaattc ttggcgatgg agttgtgcac
     3721 aatagcttcg gtcaaaaact gatgcgtatt tacaatcaga aaggcatctt ttcaaacaca
     3781 aaagattccg aagaaggctt aacacatata ctctcagagc atttcgagaa tgttaaaacc
     3841 aaggttcaag gtactgtagt aatgttttcc gcttcaggga aaaaatagca tccaaccgca
     3901 gcacgttctt gcttaagacg tgctgcggca taatcccaat gattactccc tgacagggtt
     3961 cgtaggccac tcaatatcag gtgcagttga tgtatcaaca cggttcagca acacccgata
     4021 ctttttccag gcttccagca atgaggtttc ttcctccgtt gcgatttcca gatctacagc
     4081 atcctgaagt ggcgcaatat gctcactggc tacctgcatc aggctgtttt ttgtttcttc
     4141 cgcctctcgg atccggaaca gtttttctgc ttctgcatct ttcacccagg ctgtgccgtt
     4201 ccacttctga aactcccctt ccggcgataa ccaggtaaca ttttccggta acggaccgag
     4261 ttcagaaata aataacgcgt cccctgacgc tacgtcataa accgttttac cccgatggtc
     4321 tt
//
LOCUS       DAZGSP010000212         4195 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZGSP010000212 DAZGSP010000000
VERSION     DAZGSP010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.214"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..845
                     /gene="yibB"
                     /locus_tag="ACHK74_005079"
     CDS             <1..845
                     /gene="yibB"
                     /locus_tag="ACHK74_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFP0223731.1"
                     /translation="TIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLAALENE
                     MIIFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRLEPRQL
                     KNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFP
                     FDESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLVLESQK
                     ITLNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIF
                     LSRK"
     gene            1120..2316
                     /gene="kbl"
                     /locus_tag="ACHK74_005080"
     CDS             1120..2316
                     /gene="kbl"
                     /locus_tag="ACHK74_005080"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFP0223732.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            2326..3351
                     /gene="tdh"
                     /locus_tag="ACHK74_005081"
     CDS             2326..3351
                     /gene="tdh"
                     /locus_tag="ACHK74_005081"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFP0223733.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            3590..>4195
                     /gene="waaH"
                     /locus_tag="ACHK74_005082"
     CDS             3590..>4195
                     /gene="waaH"
                     /locus_tag="ACHK74_005082"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFP0223734.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNA"
BASE COUNT         1064 a          906 c         1080 g         1145 t
ORIGIN      
        1 ccacaattat cactgcatat tttgacattg gtagagggga ttggactgct aataaagggt
       61 tccgtgaaaa actagctcgt tcagttgatg tttattttag ttactttgag cgtctggccg
      121 cacttgaaaa tgagatgatt attttcactt ctcccgattt gaaaccaagg gttgaagcga
      181 ttcgcaacgg aaaaccaaca acggttatcg ttatcgatat aaaaaagaaa tttagatata
      241 tcagaagtcg tatcgaaaaa attcaaaaag atgagtcgtt tacaaataga cttgagcctc
      301 gacaattaaa aaacccagag tactggtcac cagagtatgt tttggtatgt aaccttaagg
      361 cctattttgt aaataaagct atcaacatgg gcttagtgaa aacgcctctt gttgcgtgga
      421 tagattttgg ctattgccgt aagccaaatg taactcgagg attgaaaatt tgggatttcc
      481 catttgatga aagtaagatg catcttttca ctataaaaaa agggcttact gtcacatctc
      541 aacaacaggt ttttgatttc atgattggca atcatgtata tattattggg ggcgcaattg
      601 ttggttcaca gcataaatgg aaagaattct ataaattagt tctggaaagt caaaaaataa
      661 cactaaataa taacattgtg gacgatgacc agggcatttt tgtcatgtgc tattacaaac
      721 gccccgatct cttcaaccta aactatctgg ggagaggaaa atggttcgat ttgtttcgct
      781 gcttcaggag taacacttta ggggcaaaaa tgcaggcgct gagaattttc ctttcaagaa
      841 aataggtttc cctccattag tggatagcgt tttgatacca actgagatag gaaaaatgat
      901 gctttggcat cattttttct ttttatatca atgcagtaaa atcaatcatc attatgccca
      961 gccaaaactg tacagttttt ctactatctg taggtgttga gagacatcaa gttacacgtt
     1021 atttatcctg aattttgcag aagtgttaac gcgtcatctc gtcgcgacct ataagtttgg
     1081 gtaatatgtg ctggaatttg ccctgtctgg agaatcgcaa tgcgtggaga attttatcag
     1141 cagttaacca acgatctgga aaccgcacgg gcggaagggt tgtttaaaga agagcgcatt
     1201 attacgtctg cgcagcaagc agatatcact gtggctgatg gaagccacgt cattaacttt
     1261 tgtgccaaca actatctcgg gctggcgaat catcctgatc tgattgcggc ggcaaaggcg
     1321 ggaatggatt ctcacggttt cggcatggct tcggtgcgtt ttatttgcgg cactcaggac
     1381 agccataaag agcttgaaca aaaactggcg gccttcctgg ggatggaaga tgcgattctc
     1441 tactcttcct gctttgatgc taacggtggc ctgtttgaaa cgctgctggg cgcggaagac
     1501 gccattatct ccgacgcact gaaccacgcg tctattattg atggtgtgcg tctgtgtaaa
     1561 gctaaacgct atcgctatgc caacaacgat atgcaggagc ttgaagctcg tctgaaagaa
     1621 gcgcgtgaag ccggtgcgcg tcatgtgctg attgccaccg atggtgtatt ctcgatggac
     1681 ggcgtgattg ccaatctgaa gggcgtttgc gatctggcag ataaatacga tgccctggtg
     1741 atggtagacg actcccacgc ggtcggtttt gtcggtgaaa atggtcgtgg ttcccatgaa
     1801 tactgcgatg tgatgggccg ggtcgatatt atcaccggta cgcttggtaa agcgctgggc
     1861 ggggcttctg gtggttatac cgcggcgcgc aaagaggtgg ttgagtggct gcgccagcgt
     1921 tctcgtccgt acctgttctc caactcgttg gcaccggcca ttgttgccgc gtccatcaaa
     1981 gtactggaga tggtcgaagc gggcagcgaa ctgcgtgacc gtctgtgggc gaacgcgcgc
     2041 cagttccgtg agcaaatgtc ggcggcgggc tttaccctgg cgggagccga tcatgccatt
     2101 attccggtca tgcttggcga tgcagtagtg gcgcagaaat ttgcccgtga gctgcaaaaa
     2161 gagggcattt acgtcaccgg tttcttctat ccggtcgttc cgaaaggtca ggcgcgtatt
     2221 cgtacccaga tgtctgcggc gcatacccct gagcaaatta cgcgtgcagt agaagcattc
     2281 acgcgtattg gtaaacaact gggcgttatc gcctgaggat gtgagatgaa agcgttatcc
     2341 aaactgaaag cggaagaggg catctggatg accgacgttc ctgtaccgga actcgggcat
     2401 aacgatctgc tgattaaaat ccgtaaaaca gccatctgcg ggactgacgt tcacatctat
     2461 aactgggatg agtggtcgca aaaaaccatc ccggtgccga tggtcgtagg ccatgaatat
     2521 gtcggtgaag tggtaggtat tggtcaggaa gtgaaaggct tcaaaatcgg tgatcgcgtt
     2581 tctggcgaag gccatatcac ctgcggtcac tgtcgcaact gtcgtggcgg tcgtacccat
     2641 ctgtgccgca acaccattgg tgtcggtgtt aaccgtcctg gctgcttcgc cgaatatctg
     2701 gtgattccgg cgttcaacgc tttcaaaatc cccgacaata tctccgacga cctggcttcc
     2761 atttttgatc ccttcggtaa cgccgtgcat accgcgctgt cgttcgatct ggtgggcgaa
     2821 gatgtgctgg tttctggtgc aggtccgata ggtattatgg ctgcggcggt ggcgaaacac
     2881 gttggtgcac gcaatgtggt gatcaccgat gttaacgaat accgccttga gctggcgcgc
     2941 aaaatgggta tcacccgtgc ggtcaacgtc gctaaagaaa accttaatga tgtgatggca
     3001 gaactgggca tgaccgaagg ctttgatgtc ggtctggaaa tgtccggtgc gccgccagcg
     3061 tttcgtacca tgctcgacac catgaaccac ggcggtcgca ttgcgatgct gggtattcca
     3121 ccgtccgata tgtctatcga ctggaccaaa gtaatcttta aaggcttgtt cattaaaggt
     3181 atttacggtc gtgagatgtt tgaaacctgg tacaagatgg cggcgctgat tcagtcaggt
     3241 ctggatctct cgccgatcat tacccatcgt ttctctatcg atgatttcca gaagggcttt
     3301 gacgctatgc gttcgggcca gtccgggaaa gtaattctga gttgggatta acacgaacaa
     3361 gggctggtat tccagccctt ttgcctgagg ataatccgtt aaatatgtaa aatcctgtca
     3421 gtgtaataaa gatatcgtaa ttgcgctgat ctcttatata gctgctctca ttatctctct
     3481 accctgaagt gactctctca cctgtaaaaa taatatctca caggcttaat agtttcttaa
     3541 tacaaagcct gtaaaacgtc aggataactt cagaggtcgt cggtaattta tgatgaacag
     3601 caccaataaa cttagtgtta ttattccgtt atataatgcg ggcgatgatt tccgcacttg
     3661 tatggaatct ttaattacgc aaacctggac tgctctggaa atcattatta ttaacgatgg
     3721 ttcaacggat aattctgttg aaatagcaaa gcattacgca gaaaactatc cgcacgttcg
     3781 tttgttgcat caggcgaatg ctggcgcatc ggtagcgcgt aatcgtggga tcgaggtggc
     3841 gacgggcaaa tatgtcgctt ttgtcgatgc tgacgatgag gtctatccca ccatgtacga
     3901 aacgctgatg accatggcgt tagaggatga cctcgacgtg gcgcagtgca acgctgactg
     3961 gtgttttcgt gaaacgggag aaacctggca atccatcccc agcgatcgcc ttcgctcaac
     4021 cggcgtatta accggcccgg actggctgcg gatggggctt tcttcgcgcc gttggactca
     4081 cgttgtctgg atgggggttt atcgccgtga tgttattgtt aaaaataaca ttaaatttat
     4141 tgccggatta catcatcagg atattgtctg gacaacagaa ttcatgttta acgcg
//
LOCUS       DAZGSP010000213         4141 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZGSP010000213 DAZGSP010000000
VERSION     DAZGSP010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.215"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..904)
                     /locus_tag="ACHK74_005083"
     CDS             complement(<1..904)
                     /locus_tag="ACHK74_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP0223735.1"
                     /translation="MKINRHGITLSLISFLIAGAPAFAQDDSSWASPGTASASETSST
                     AVGAWARAGKAGTALGSASYAHAPFSTSVGMSAYVNPKASNGVAVGSWAKADALNSVA
                     LGTGAQATHRYSVALGQQSGTTRENEVYIGYDSGVTNPASPRVPDKTRVLGGVSDGTR
                     DTDAATVGQLNRKADEVYSDVSGRIAAEALKARDHTDTVAAENRENIIRNTVAINRNT
                     RGLLSQRDVLETHEERLNSQQQQINTGSTVAVDSHGYVTRGEGTGERITVQEGLVRTQ
                     EMATENRAAVSRNRQVGERNSRAIA"
     gene            complement(1166..3034)
                     /locus_tag="ACHK74_005084"
     CDS             complement(1166..3034)
                     /locus_tag="ACHK74_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223736.1"
                     /translation="MGLFNYKSYASTQIRELVSGSYNLAIYSNTGKVLGLGSFLASRL
                     FAKHPDRVDYSTDVLNEGWRDITVEELRLSSEYVDSRGFYRFPCPVTGNPASGFGPQL
                     KVVGLFDAQQNLQKIAISFCGTNDICDIVDYTQLNNGKALSEAENLLQSIRDFAVSNG
                     LSGEDVIVTGYSLGGGLTNIMARLSESLADGFYANSDYIAWASPLVFDGDGKVLNIGF
                     ENDPVFRVLGDESGFWEAVKAMGPGLTTPDYEYDSSPDNIVLYTDLYGSFLWNIIPFS
                     VLALPLGSWSAHFAGSKTDAFERILGSQFYDYMSPDSTVIVDEQSGWTSWYSWVHDKG
                     DDSRERPVFIIGNDGNNLLAGGQGGDYIEGAGGNDKIEPGKGANRVDGGSGYDTLLLS
                     DVMDDWNIYRVSDGTLFFHSERGAGLTEATGIEGISFSGDWRSKLSPYQIEEEQITDH
                     RYLLKWRNKNVDYGKHTEGTNGDDSLFGNIIFGLDGNDILHSAGGEGVSLLHGGRGND
                     YLHGTSGNDELYGAEGNDWLYGGGGNDYLYGGTGHDVFVFDAACEGNSFIMDFNKHKS
                     EEDFLFLSKDIVTSTDEFISCLKQTGNNVEAGIAGGRIYFVNTALEDINHNSVYIV"
     gene            complement(3098..>4141)
                     /locus_tag="ACHK74_005085"
     CDS             complement(3098..>4141)
                     /locus_tag="ACHK74_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223737.1"
                     /translation="FNNAKVHLEKALNTVIQPNDDLYAVYKLAFGKPVGNPEESTTTW
                     REVLNQNGVSGFSVDKNYGGEFRYTYNGLEGSDIISTGYSIGGTARTMISRNDMVINT
                     NGGDDIIAVGMDVGRSYSAGYTDKKYLTSTGDGDDILIVGLGNNNVGIHLKDDASLRI
                     SDRDDVISDGELLLPSSSYDHGSGGIISSTTITMGDGDDTLLALGYERFGNAIVNTDI
                     DLGAGNDTIHVNGDVKAVSIKGGSGMDILAISHGTLSTKDFSGFEVIDLGDNNGRVNI
                     IASDLVKSGVDAIEGGTLKIIGSSNSGVNLDGDDWKSDGVQSEDGVNYNVYTHEAAPD
                     IKILIDEHITNVM"
BASE COUNT         1226 a         1090 c          701 g         1124 t
ORIGIN      
        1 aagcaatggc gcgactgttg cgttcaccca cctgacggtt acggcttacc gcagcacggt
       61 tttctgtggc catttcctgt gtacgtacca gcccttcctg tacagtaatt cgttcaccgg
      121 ttccctcacc gcgcgtcaca taaccatgac tgtccacagc aacggtactt ccggtattta
      181 tctgctgttg ctggctgttc aggcgctctt catgcgtctc cagcacgtct ctctgggaaa
      241 gcaaccccct ggtgttacgg tttatggcca ccgtgttccg gataatattc tccctgttct
      301 ctgcagcaac cgtatcggta tggtcacgcg ccttaagtgc ttccgctgca atccgtccac
      361 tgacgtcact gtacacctca tccgccttcc ggttcagttg cccgacagtg gctgcatccg
      421 tatcccgtgt accatcactg acaccaccga gaacccgggt tttatccggt acacgcggac
      481 tggccgggtt tgtgacacct gagtcatatc cgatgtagac ttcattttca cgggttgtgc
      541 cggactgctg gccaagagcc actgaatatc tgtgcgttgc ctgcgcaccc gtacccagtg
      601 ccaccgaatt taaagcatca gcctttgccc agctacctac tgccacacca ttagacgcct
      661 tcggatttac gtaagctgac atccccacgg atgtggaaaa cggcgcatgc gcatacgatg
      721 cagagccaag agctgtccct gcctttccag ccctcgccca tgcacctaca gccgtggagc
      781 tggtctctga tgcagaggct gtgcccggag acgcccagga actgtcatcc tgcgcaaacg
      841 ccggagcacc ggcaatcaga aaggaaataa gcgataaagt aataccgtgt cgatttattt
      901 tcataaacaa cctcattgat atacacaata tactgacaca gagttaaatt aatatataat
      961 caggagaaaa gtgaaagagc acaggtgcga aaatgaatta atctgagcgg atttaacaat
     1021 aaatgaagtg caatgaatta aaattaaaaa caaaaatcac ttgcagtata ttttttgcga
     1081 cacccatcgc aaccgcacac aaaaccaaac gtacaatgca ttgattttaa acaaaaaaac
     1141 aaaacagaaa ctcaacacat aaaaatcaga caatataaac agaattgtga tttatatcct
     1201 caagcgctgt attaacgaaa tatatccgtc ctcctgcaat acctgcctca acattatttc
     1261 cggtttgttt aagacatgaa ataaactcat ctgtcgatgt aacaatgtcc tttgataaaa
     1321 acagaaaatc ttcttcactc ttatgtttat taaaatccat gataaatgaa ttcccttcac
     1381 aagcagcatc aaagacgaag acatcatgac cagtaccacc atacagataa tcattacctc
     1441 ccccaccata taaccagtcg ttaccttctg ccccgtacaa ttcatcatta ccagaagtcc
     1501 catgaagata atcattcccg cgacctccgt gtaaaagact tactccttct ccccctgcac
     1561 tatgtaatat atcatttccg tcaagaccaa aaataatatt tccgaacaga ctatcatctc
     1621 cgtttgtacc ttctgtatgt tttccgtaat caacattttt attacgccac ttcagaagat
     1681 acctgtggtc tgtaatctgc tcttcttcta tctgataagg gcttagttta ctcctccagt
     1741 caccagaaaa agagatacct tcaatacctg ttgcctcagt cagaccggct ccccgttctg
     1801 aatggaaaaa cagcgtccca tcactgaccc tgtatatatt ccagtcatcc attacatcgc
     1861 taagaagcag cgtgtcatac ccactgccac catcaaccct gtttgctccc ttacctggtt
     1921 caatcttatc attccctcca gccccctcta tatagtcacc accctgcccg cctgccagca
     1981 gattattgcc atcattaccg atgatgaata caggtctttc acgactgtca tcccccttgt
     2041 catgaaccca tgaataccag gatgtccagc cactctgctc atccacaatg actgtactgt
     2101 ccggtgacat gtagtcgtaa aactgagacc ctaaaattct ttcgaaggcg tcagtttttg
     2161 aacccgcaaa atgcgcactc cagcttccca gcgggagtgc aagcacagaa aaggggataa
     2221 tattccagag gaatgaacca taaaggtctg tgtataaaac aatattatca ggagaagagt
     2281 catattcata atccggggtt gtcaagcccg gccccattgc tttaactgcc tcccagaacc
     2341 cggattcatc tcccagcaca cgaaataccg gatcgttttc aaaaccgata tttaatacct
     2401 tcccatcccc gtcaaatacc agcggggaag cccaggctat atagtcagaa ttagcataaa
     2461 aaccatcagc cagagactca ctgagtctgg ccataatgtt agtcaacccg ccccccaggc
     2521 tatagcctgt gacaataaca tcttcaccac ttaaaccatt actgactgca aagtctctga
     2581 tactttgaag caggttttct gcttcagaca gcgcttttcc attattgagc tgtgtataat
     2641 caacaatatc gcagatatca tttgttccac agaatgatat tgcaattttc tgtagattct
     2701 gttgagcatc aaacaaccca acaactttca actgaggacc aaatcctgac gcagggtttc
     2761 ctgtaaccgg acagggaaag cgataaaaac ctcttgaatc gacatattcc gaagataacc
     2821 ggagttcttc cactgtaata tcacgccagc cttcattaag aacatcggta gaataatcaa
     2881 ctctatctgg gtgctttgca aatagcctgg atgccagaaa acttcccaat ccaagaacct
     2941 ttccagtgtt ggaatatata gcaagattat aagagccaga aacaagctct ctaatttgtg
     3001 ttgacgcata agatttgtaa ttaaataacc ccatacaaac accttgatgt tgaaaaaaac
     3061 tggatagaaa gagccccata ggggctcttt tataagatta cattacattc gtaatgtgtt
     3121 catcgattaa tattttaatg tctggcgctg cctcatgtgt atacacatta tagttaacac
     3181 catcttcaga ttgaactcca tcagacttcc agtcatctcc atctaagttt acacccgagt
     3241 tagatgaccc aataatttta agtgtgcctc cctctatagc atcaactcca cttttcacaa
     3301 ggtcagatgc aattatattc acccttccat tattatcacc aaggtcaata acttcaaacc
     3361 cagagaaatc tttagttgaa agggttccat gactaatggc cagaatatcc ataccagagc
     3421 cacctttaat acttaccgct ttcacatcac cattaacatg aatagtgtca tttcctgcac
     3481 ctaagtcaat atcggtatta acaatagcat taccaaatct ctcatagcct aaagcaagaa
     3541 gtgtatcatc accatcaccc atagttatgg tggtagacga tataataccg ccactaccat
     3601 gatcatatga acttgatggt aataacagtt ctccatcact aatcacatca tctctatcac
     3661 taatacgtaa acttgcatca tctttcagat gtataccaac attattatta cctaggccga
     3721 caatcagaat gtcatcacca tctcccgttg atgtcaaata ttttttgtca gtatagccag
     3781 cactatagct tctaccaaca tccataccaa cagcaataat atcatcgcca ccgttagtat
     3841 taataaccat gtcattacga cttatcattg ttctggcagt gccaccaata gaatagcctg
     3901 ttgatatgat atcgctgccc tccaatccat tataagtgta tcggaattct ccaccataat
     3961 tcttatcaac actgaagcca gaaacaccat tttgattaag aacttcacgc catgtagtag
     4021 tgctttcttc tggattacct acaggcttac caaaagcaag tttatataca gcataaaggt
     4081 catcatttgg ctgaatgaca gtatttagtg ccttttccaa atggactttt gcattattaa
     4141 a
//
LOCUS       DAZGSP010000214         4126 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZGSP010000214 DAZGSP010000000
VERSION     DAZGSP010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.216"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..353)
                     /locus_tag="ACHK74_005086"
     CDS             complement(<1..353)
                     /locus_tag="ACHK74_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin"
                     /protein_id="HFP0223738.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPG"
     gene            complement(498..1847)
                     /gene="lysC"
                     /locus_tag="ACHK74_005087"
     CDS             complement(498..1847)
                     /gene="lysC"
                     /locus_tag="ACHK74_005087"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFP0223739.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     regulatory      complement(1939..2135)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2372..4021
                     /gene="pgi"
                     /locus_tag="ACHK74_005088"
     CDS             2372..4021
                     /gene="pgi"
                     /locus_tag="ACHK74_005088"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFP0223740.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRD"
BASE COUNT         1059 a         1056 c         1035 g          976 t
ORIGIN      
        1 ccgggttgcc cacctaaaac aaaaccaatg ggatgctgtt gcggtgtgac ttcatgattt
       61 gtcagacgct gccaaataga atcaaaagat gctttatttt ctgaatgcgt agtattgttg
      121 aattcatgct gatattgagt tcttacctgc tgaaggagaa tcttaccttg tgtaaaatca
      181 acaagttcag cgggggtaga ataaagataa tcgcgatagc tgactaatcg cttgagagca
      241 tcgcctgaat cctgatgcga cgaaactgag caattatcaa tgatatttga taaaaacgcg
      301 atgcactgac tggctccgtt taaaagtgca tctttttcca ttaaagtagc cattaacact
      361 cctgttaaat gatatttttc ggtgattata atactgttct acaggagtga ttttgctttt
      421 cagatattat ttaatctatg ttttcagtga ggatggcgta taacaatcgg tgagcgttat
      481 acgccagtag ggaaatatta ctcaaacaaa ttaaaatgca gcttctgcac cacctgctcg
      541 gcatcttcgc cgggcaccag gaagcacagg ttatggctgg atgcgccgta acaaatcatg
      601 cgaatgttga acggttccag tacgccgaat acctctttgc caacgccgca ggcttttgac
      661 aggtcattgc caatcaacgc gaccagcgcc agaccttctt ctacttccac ccggcacagt
      721 gcggaaagct ccatcagcag agattgcgtc agcaacgtat cgccagtgga ggttgaaccg
      781 gtggtatcaa gggttaatgc cacgctcact tctgacgtgg tgattaagtc taccgaaata
      841 ttatgccgcg cgaggatgcc gaaaacttcc gcgaggaagc cgcgagaatg cagcatattc
      901 aggctgtgca aagtgagcag agtctgattg cgacgaagcg ccagcgcgcg gaacagcggc
      961 ggattttcag ttttattgca caccagcgta ccacctgcgc gtgggtcttt gctggagccg
     1021 acaaagaccg ggatatcgct gcgtactgcg ggtagcaacg ttgccggatg cagtactttt
     1081 gcaccaaaag ttgccatctc tgccgcttcg gcaaacgcga tttcatcaat gcgttttgct
     1141 gcggaaacta cgcgtggatc ggtggtgtag atgcccggga cgtcggtcca gatatcaaca
     1201 cgagatgcgt gtaaagcctc cgccagcaag gctgccgtat aatcgctgcc tccacggcca
     1261 agcgtcgttg tacgaccttt attttcgcta ccgataaatc cctgggtgat cactaagccg
     1321 tcattgaggc gtgggagcag ctgcagcgcg gccagttccg ccagcgcggc tacatctggc
     1381 tctgcacgac caaatcggtc gttggtacgc atcactttac gtacatcaaa ccactgtgcc
     1441 tgaacatcgc gttcgcgcag gatttcgaca aacagcaggg tcgacatcag ctcgccgtgg
     1501 ctgaccagct catctgtcag cgccggagac gttgccagcg ccgccgcttc tgccagaaca
     1561 gtaatgttct ccagcagacg ttcaatctct tcacggataa cgttcgggta acgcagacgt
     1621 tccagaatgg caaactggat gttgcgaata gcgtcgagtt tttcgaatcg ctcgccaggt
     1681 tccagccctt ccgctaaagc gaccagcaga ttagtgatac cagcagaagc cgagaggact
     1741 actaaacgca cgttggcatc agaaagcaca atatcagcgc tgcggttcat ggcgtcaaaa
     1801 tcagctacgc tggtaccgcc aaatttggag acaacaattt cagacataac tacctcgtgt
     1861 caggggatcc attttcagcc ttggcacaag ggaagagcgg aagacgggtg ggcgcagagc
     1921 gatacttcgc tactattttc acccagaagt gctccaccac ttgcgaaacg cccgactgcg
     1981 aacgcttctg gtgacaaccc aggggattca gcccctgtag ccgatgatga acgtggccag
     2041 ccgttcaatc acctcggcga tgcaccccct caggtgttat cacaggactg gctcctccaa
     2101 caccgttact tgggcaacgc gcctcttctg gcctgcgcta gcgcaggtag tacatttata
     2161 aataaagggt gagcggggcg gttgtcaacg acggggtcat gcggattttt catccactcc
     2221 tggcggtcag tagttcagct aataaatgct tcactgcgct aagggtttac actcaacatt
     2281 acgctaacgg cactaaaacc atcacatttt tctgtgactg gcgctacaat cttccaaagt
     2341 cacaattctc aaaatcagaa gagtatcgct aatgaaaaac atcaatccaa cgcagaccgc
     2401 tgcctggcag gcactacaga aacacttcga tgaaatgaaa gacgttacga tcgccgatct
     2461 ttttgctaaa gacggcgatc gtttttctaa gttctccgca accttcggcg atcagatgct
     2521 ggtggattac tccaaaaacc gcatcactga agagacgctg gcgaaattac aagatctggc
     2581 gaaagagtgc gatctggcgg gcgcgattaa gtcgatgttc tctggcgaga agatcaaccg
     2641 cactgaaaac cgcgctgtgc tgcacgtagc gctgcgtaac cgtagcaata ccccgatttt
     2701 ggttgatggc aaagacgtaa tgccggaagt caacgcggtg ctggagaaga tgaaaacctt
     2761 ctcggaagcg attatctccg gtgagtggaa gggttatacc ggcaaagcaa tcactgacgt
     2821 agtgaacatc gggatcggcg gttctgacct cggcccgtac atggtgaccg aagcactgcg
     2881 tccgtacaaa aaccacctga acatgcactt tgtttctaac gtcgatggga ctcacatcgc
     2941 ggaagtgctg aaaaaagtaa acccggaaac cacgctgttc ctggtagcat ctaaaacctt
     3001 caccactcag gaaaccatga ccaacgccca tagcgcgcgt gactggttcc tgaaagcggc
     3061 aggtgatgaa aaacacgttg cgaaacactt tgcggcgctc tcaaccaatg ccaaagccgt
     3121 tggcgagttt ggtattgata ctgccaacat gttcgagttc tgggactggg tcggcggccg
     3181 ttactctttg tggtcagcga ttggcctgtc gattgttctc tccatcggct ttgataactt
     3241 cgttgaactg ctttctggcg cacacgcgat ggacaagcat ttctccacca cgcctgccga
     3301 gaaaaacctg cctgtactgc tggcgctgat tggcatctgg tacaacaatt tctttggtgc
     3361 ggaaactgag gcgattctgc cgtatgacca gtatatgcac cgtttcgcgg cgtacttcca
     3421 gcagggcaat atggagtcca acggtaagta tgttgaccgt aacggtaacg ttgtggatta
     3481 ccagactggc ccgattatct ggggtgaacc aggcactaac ggtcagcacg cgttctacca
     3541 gctgatccac cagggaacca aaatggtacc gtgcgatttc atcgctccgg ctatcaccca
     3601 taacccgctc tctgatcacc accagaaact gctgtctaac ttctttgctc agactgaagc
     3661 gctggcgttt ggtaaatccc gcgaagtggt tgagcaggaa tatcgcgatc agggtaaaga
     3721 tccggcaacg cttgactacg tggtgccgtt caaagtgttc gaaggtaacc gcccgaccaa
     3781 ctccatcctg ctgcgtgaaa tcactccgtt cagcctgggg gcgttgattg cgctgtatga
     3841 gcacaaaatc tttactcagg gcgtgatcct gaacatcttc accttcgacc agtggggcgt
     3901 ggagctgggt aaacaactgg caaaccgtat tctgccagag ctgaaagatg ataaagaaat
     3961 cagcagccac gatagctcga ccaatggtct gattaaccgc tataaagcgt ggcgcgatta
     4021 atttaaaaac cttaataaca atgccgacgt tatgtcggca ttgttttatc agataaatcc
     4081 ccttgtctgt gatttaacgg aaatcatacc gtgaggttaa tcctaa
//
LOCUS       DAZGSP010000215         4018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZGSP010000215 DAZGSP010000000
VERSION     DAZGSP010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.217"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..739)
                     /locus_tag="ACHK74_005089"
     CDS             complement(<1..739)
                     /locus_tag="ACHK74_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFP0223741.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSND
                     KDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDE"
     gene            complement(881..2788)
                     /locus_tag="ACHK74_005090"
                     /pseudo
     CDS             complement(881..2788)
                     /locus_tag="ACHK74_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
     gene            complement(2808..3269)
                     /locus_tag="ACHK74_005091"
     CDS             complement(2808..3269)
                     /locus_tag="ACHK74_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFP0223742.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(3297..>4018)
                     /locus_tag="ACHK74_005092"
     CDS             complement(3297..>4018)
                     /locus_tag="ACHK74_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP0223743.1"
                     /translation="DRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNG
                     DPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIH
                     AQKNMDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSE
                     DVFQDRGTIAGSVHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGE
                     STVIRLSKDGITIVGGSVFIN"
BASE COUNT          834 a         1128 c          995 g         1061 t
ORIGIN      
        1 gttcatcagg gacatcgcaa acaatatgaa gacggatttc ttctctgccg tgactctttt
       61 cactgattgc gtaactgtca tgctctggat tatttaattc tttcagcgga aatttttcct
      121 caaaggcttt atttagccgc ccctggtttc cttttaccgc gaataaataa tcacctccct
      181 gtttttgtat cttctctgca atatctttct ggcaacccat cgcatcagtt gtgatgattt
      241 ttcctttaat atccagcatg ttaagaagtt ctgggatagc tgtaatctca ttagatttct
      301 catccgtctt gatctgtccg atgaccagac tgtgcattgt tgagaacgca ctaatgacat
      361 gaatcgctcc cctgcggcga ctcttgtcat aagagtgccg gagcgttttt ccatcaattg
      421 caatgacgtc tttatcattt gatgaatggc agtcacgcat ccagttaata aagcactcgt
      481 gaaattttgc aggactgata caggatacaa ctctggcaat ggtatcgtga acaggaatac
      541 cattttcaaa atcaccatat tgcttcaaaa aatcgagatg tgtttcccca aaatcctcta
      601 tatcttccca accttctgca ccagaaataa cggcgcaaat agtcaacagt agaatatccg
      661 ataatttatg ttccactttc caggcttgtc tgtaatcggg aataatagaa atatgttcca
      721 tcaatttttt aagttccatt ttgttctcct taattatgta aggagtattt gatcatgtat
      781 aagcaataaa aaacagcttc aggtaatgag gaatatctca atttttaaac ataaaatgtg
      841 aattatttag tacaaaaaac cggcgttaca tgatctttcc ctggctccac gtctggtcgt
      901 tatcggacca gcggatgatc cggtccgcag cgtcgtactc ataaaacagg tggtagtcca
      961 gccgcgcatc cgcttctgtc agccgccccc gcgcatcctg ttcgtaggtc gccagcgtct
     1021 gtatgccgtt atccgtacgg tgaatggctt tcaggtagcc gctggcgtgg tacgtcagtt
     1081 taagccggat cccgtcggtg tgactgacac ccgtgatccg gtactggctg tcgcgctcaa
     1141 acgtgatgcg gttgctggcg gtgtcgctca tcccgcccag catgtacatc cggtcattgg
     1201 ggctgtgtgc caggcggtca tcgctggtat cgtcagtcac cgggtggcgc agacgcattc
     1261 ccggaaaggc gtcataaaag aagcggctgc tcagagcatc cctgtcgaac agctccagat
     1321 aatcatcgcg gcggcgcaga atgtactggg cgtgatacgg gttaaccgtc gtatcactgt
     1381 caccgtcgaa ggcaaatctc agcgtggctc cctggctgat gatatccacc cggtttccct
     1441 gttcacgcac ccaggcgtat tcgctccagg agtcgctcca gcccacgccc gtcaggccat
     1501 gcagcggcag tacagaactg tggaaacggg tgaaggagag ggggagggtc tggcccagcg
     1561 taaagtcggt acgttcgtcg cagtatgcgc cggttaccgg atcgacagga tctgttaccc
     1621 atctgatgac tttattggca atacttttta acgacttacc tgttttcccc agcaacgcgc
     1681 cgagctttgc gcccagcagc ttcccgcttt tccccagcag gtttcccagt ttgccgagtc
     1741 tcgccagatt cttcagtttt cccagcagtc ccgcaggcgg cagaagatag ctggctatcg
     1801 ttattccccc caggatcatt ctctgccacg gcgggaactc cggctggata tccagatagg
     1861 tcacctgctc accgccaata aagacgtccg gcgaacctcc cacgatcacc gcactgcact
     1921 ccagtttgtc gcccttgcgc gcggcaggct gattgttgat aaagacgttg ctgctgccct
     1981 gggctatcct cggtggtttg ttctccttgt cgcagagcgc cgtgtcctca ccggcttccg
     2041 ggaaatccac ctcttttttg atttcgccac gcccgcccac catggtggca aactccatta
     2101 ataccggacc ggcgggaaac agaagcgatg cgccggcgct gacgcgatcc ataaaaccgg
     2161 cattgctgtc cgtcagggtg ataacagcgt tcccccaggc cttcgccagt ggtactgctt
     2221 tttccgctgc cgattcaagc aggtcagtgg caatatcacc ccatgagggt tccccctgtg
     2281 ggacttcagg tattgctcca ggtgggggag gagggggaag ggtgaccgct gcacgtgcgg
     2341 ccagctcgcc gttaattttt acgttttcag aaccggtatt gatggcgccg tccgtgtcac
     2401 aaaagctgtt tatccatgat tcaagctggt tctgtttctc ctccacataa tcactcgcct
     2461 tatagctggc atacatcagc gcaggcacca gaaccagcgc ataagggccg gtggcacagg
     2521 cggccataat caggcaacct tctgcgatgg tcactatcgc ccccagcacg gcccctgcca
     2581 gcgcccccaa aaaactcttg tgctgaattt catcgcccag cctggcggcg ggggggccgg
     2641 acttttttga tgccagcatc tgtgcaatgg ctttgtcgtt ggctgcccgt ttcatctggc
     2701 tggcgaccat cgcctgctgt gacagcattc gcattgccag cgtaccgcct gcggtcgctc
     2761 cctgtggccc gcctggtcct tcactcatgt gttatccctc gtttatgtca gcgcggctgg
     2821 tccttttgga tgtcattctc cggtggcaac ggggtgaagc tctttatcac ctccagcagc
     2881 atcgtctttt gactgttctt cagggataag gaggtgctga aagtgaaggt cagcagcgta
     2941 tctccacgcc gctggaatac ggctatctgg ctaatccgcc cctgattccc ctcatcttcc
     3001 ggaccgttcc aggaataatc cagcagccag gccggggcct ctgccagagt gatttcccgg
     3061 caggcatgtt cggtgtaccc atcaagtttg ccctggtaca gcgccagcgt ttcctggtac
     3121 agctctggtg tcagggttcc ctgtggaacg gcttcgcggg taatgaccag gttgatgcca
     3181 ctgttatctg tggacaccag gacctgcatt gactgatcct gccattgaga cggcagttga
     3241 atgtggcctt cattaaaagt gaactgcata aaatactccc tgataatctg agtgaatcag
     3301 ttgatgaaaa cagaaccgcc cacgatggtg atgccatcct tcgacagcct gattaccgtc
     3361 gattccccga cctgtagctg aatgccatcc ggcgtcagcg caattttgct ttcctccacg
     3421 gccagcaaaa tttccttaat cgccgtataa gcctgtatac cgtcgccttc gccaccattg
     3481 tttacagcgt caacatgcac cgaaccggca atcgtgcctc tgtcctgaaa gacatcctca
     3541 gataaagacc ctttcagtac tccggtgatc ttgtggcctt tttccaccgt aagaaggcgg
     3601 ttaccttcct ggatcgtgac cgtctggtca ttttttacgg tttcgcggtg atcgtgtttt
     3661 acggtggtgg tacggtcgtt gagcacttcg gtatccatgt tcttctgcgc gtggatgtag
     3721 acctgttctc tccctgtcgc atcatcaaac ttcagctcat taaatccgct gcccatgtac
     3781 gttttggagc ggatggtcat ctgcgtcttt gttcccggca ggctgccggg ggtgcggttt
     3841 tcctggtggt aggtgcgccc cataatgatc ggctggtccg gatcgccgtt gaggaagtct
     3901 acaatcacct cctgacccac acgcggtatc gccaggtggc caaaaccggt gcctgcccac
     3961 gcctgtgcca cacggatcca gcacgaactg tcttggtctg ccgggttata acggtccc
//
LOCUS       DAZGSP010000216         3918 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZGSP010000216 DAZGSP010000000
VERSION     DAZGSP010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.218"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..359)
                     /locus_tag="ACHK74_005093"
     CDS             complement(<1..359)
                     /locus_tag="ACHK74_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFP0223744.1"
                     /translation="MGNRKQVTSRIISTPELIRYNDNIVGYGSRELRVETISCWLARL
                     VIVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNSGARVVTGTQNREYMELN
                     RLWLHDCMPRNSESRAI"
     gene            complement(313..513)
                     /locus_tag="ACHK74_005094"
     CDS             complement(313..513)
                     /locus_tag="ACHK74_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0223745.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSQSATEHP
                     TYTRKSCRGEQKASHIPYHQHA"
     gene            628..1091
                     /locus_tag="ACHK74_005095"
                     /pseudo
     CDS             628..1091
                     /locus_tag="ACHK74_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1130..1375)
                     /locus_tag="ACHK74_005096"
     CDS             complement(1130..1375)
                     /locus_tag="ACHK74_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP0223746.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGRSPAENSTENEPQPVEQPEPVQPGTDGTMM"
     gene            complement(1411..1590)
                     /locus_tag="ACHK74_005097"
     CDS             complement(1411..1590)
                     /locus_tag="ACHK74_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0223747.1"
                     /translation="MTYINVCLLYFCTVVCCLLLVSGGYKIIRNYFRNKIDAAAEEKI
                     KSGAVMPQPKDSGQI"
     gene            complement(1648..3705)
                     /locus_tag="ACHK74_005098"
     CDS             complement(1648..3705)
                     /locus_tag="ACHK74_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP0223748.1"
                     /translation="MNIMTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMT
                     FADCLISFNTRDCLWVRPVSGDPNVEITGAGIGAVIPLSADVAGTTEPSDWDNAETHT
                     RPAGNETASSSPSWYYVIVIAGQSNASSYGEGLPLPDSYDRPDPRIMQLARRNTQTPG
                     GIPCKYNEIIPADHCLHDVQNMSLLNHPKADLKKGQYGCVGQGLHIAKKVLPVIPADA
                     GILLVPCARGGSAFTTGAVGSFDPASGAAEASLRWGVDTPLYQDLVSRTKAALEANPK
                     NVLLSVVWIQGEGDLKATPAQHGAQFTAMVKKFRADLGAFSGQCIGGSSDSVPWLCGD
                     TTYDWKEKNPAAYETIYSGYKNRASENIHFVPLMHDEFGNKTATNLPEHDPDIASAGY
                     YGAESRGDGNWTTASRATHFSSWARRFLVSDRFASAILKYAGLTSAFIAGVAPDGEAL
                     PQPAPGGKPEAPTGSQPGSSESPQPTSPSLRTLLSLKASAGEASAQGWKITDGTIQLT
                     DEGKALTVSKQSGKTWTLTYGTDAAGELLKHGGQLTCKFRLSGEVKEGQFALGIYLHT
                     DTVPEGVTMSGTGTPFLMAYYLQTTGGQLNLMHHKTGGNTKLGSFGAYNNQWHTLVLK
                     FTAGSAQVTPVLDGTPGQAFQMVKDALSPAKDTLTLTDVTKGATYGVSFESVVLEVNT
                     PAA"
     gene            complement(3796..>3918)
                     /locus_tag="ACHK74_005099"
     CDS             complement(3796..>3918)
                     /locus_tag="ACHK74_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0223749.1"
                     /translation="QYEQVGRLLAGGVSRRQVALIFDIGLSTLYRYYPASISEK"
BASE COUNT          993 a          990 c          900 g         1035 t
ORIGIN      
        1 ctgattgccc ttgattcgga gtttctcggc atacagtcat gcagccatag ccggttaagc
       61 tccatatatt ctcggttctg cgtacccgtt acgacgcgtg caccactgtt tgggttaagg
      121 gcgtaacccc attgcattac gccaacaagt tcccgttctg aaaatatgcc cagatgaagg
      181 taggagttat ttacgaaacg gtggctgtaa tgtttattga cgatgaccag tctggccagc
      241 cagcaactta ttgtctcaac ccgcaactca cgggagccat aaccaacgat attgtcgtta
      301 tagcgaataa gttcaggcgt gctgatgata cgggatgtga cttgctttct gttccccacg
      361 acaggatttc cttgtgtatg tggggtgctc tgtggcgctc tgagatgtga tttgattgag
      421 ggttttacag cgcggacatt ttacttccag ataactgaaa ctggcccgtg ccagtagctt
      481 attgcagtgt cggcaccgta tgtttcgata catggccgca taacctcctg ctttgttgtt
      541 gatgcctttc tcatgcaatc gatcgacaaa aacgatcgat tcgatttgtt taattgatac
      601 aacaaataga ttgaaaagtc atcaacaatg attcagggag gtgattattt acttaaagaa
      661 aacgaagagt tagcgagtga aaacgttcat aaaatgatca ttttattgac tttttatcca
      721 gtgaaggtag gatcgcgcgt atatgtagta tgtttcaaat caacaggata ctacataaaa
      781 gggaaaccca gcatagctgg aactatgctg ggtcaatctg taaaaacttg catcaatgct
      841 ttacttcgag ctcgtgtcgt atcctatgat attccccggt ctcctgtaaa gcagagatgc
      901 ataaaaagga gatctttatc atggataaag agcctgtttg ccctgcctgt aagactccct
      961 tacagctcat ctatcgccgt tctcgatctc aaaatggcaa gacatacaaa ccaaaaaatg
     1021 cttcctgttt caggatgctg atatgtcctg cctgttctcc ccggagggaa aatacacaaa
     1081 acgtgcatta acaaattcac ccggcaggga aacctgccgg tcggtattgt tacatcatag
     1141 taccgtcagt tccgggctga accggctctg gttgctctac gggttgaggc tcattttccg
     1201 tgctgttttc agccggagaa cgaccaacca ttcgggccag cacttcatca acatatgcgt
     1261 cgatttgtgc ctcaaaatcc ttgcgaacct gcgccttcag cacgcggttc acttcttcag
     1321 aataaagtgc ctgtttgaca agctcttcag taaccgtgcc tttaatctct ggcatgaata
     1381 ttctcctttc cttttctggt tcacaaaaaa ttaaatctga ccggagtctt tcggctgcgg
     1441 cattacagca ccgcttttta tcttttcctc ggcagctgca tcaattttat ttcgaaaata
     1501 attgcgaata attttataac caccgctgac aagcaacaga caacaaacca ccgtgcagaa
     1561 atacagcagg cagacgttta tatatgtcat ataagcctca ctcttattaa ctgatgttac
     1621 agccggataa attcccggcc gtcacgatta tgccgcaggg gtattaacct caaggacaac
     1681 gctctcaaaa gacacgccat aagttgcccc tttggtgaca tctgtcagtg tcagcgtatc
     1741 tttggcaggg gaaagtgcat ctttcaccat ctggaatgcc tggcccggag tgccatccag
     1801 cacaggcgtt acctgggcac ttcccgcagt aaacttcagg acaagtgtat gccactggtt
     1861 attgtaggct ccaaatgatc ccagtttcgt attcccccct gttttatggt gcatcagatt
     1921 taactggccg cctgttgtct ggagataata cgccatcagg aacggtgtgc ctgtaccact
     1981 catggttaca ccttccggga ccgtatcggt gtggaggtaa atacccagag caaactggcc
     2041 ttcttttact tcacctgaca gacggaattt acaggtcaac tgaccaccat gcttcagaag
     2101 ttcaccggct gcatcagttc cataggtaag cgtccaggtt tttccgcttt gtttggatac
     2161 ggtcagtgct ttaccttcgt ccgtcagctg gattgtacca tctgtaattt tccatccctg
     2221 agccgaggct tcccctgctg atgctttaag tgaaagcagc gtacggagtg acggggatgt
     2281 cggctggggg gactcactgc ttcctggctg actacctgtc ggcgcttccg gtttcccacc
     2341 cggcgcgggc tgaggcaggg cttcaccatc aggggccaca ccggcaataa atgcagaggt
     2401 cagacctgcg tatttaagaa ttgctgatgc aaaacgatct gagaccagaa aacggcgcgc
     2461 ccaggagcta aaatgggttg ccctggatgc tgttgtccag ttgccatctc cccgtgattc
     2521 cgcaccataa tacccggcag aagcgatatc cgggtcgtgc tccggcaggt tggtcgcggt
     2581 tttattaccg aactcatcgt gcatcagtgg aacaaagtga atgttctcgg aagcccggtt
     2641 tttatatccg ctataaatgg tttcatatgc cgccgggttc ttctctttcc agtcataggt
     2701 tgtgtcacca cataaccacg gcacactgtc tgatgaacca ccaatacact gaccggagaa
     2761 agcaccaaga tccgcacgga attttttcac catcgcagta aactgggcac catgctgggc
     2821 cggggtcgct ttaaggtctc cttccccctg aatccatacg acagataaca gcacattctt
     2881 tggattcgcc tcaagtgccg ctttcgttcg actgacaaga tcctgataca gtggggtatc
     2941 cactccccag cgtagtgacg cctcggctgc accacttgcc ggatcgaatg agccaactgc
     3001 gccggttgta aaagcagagc cgccacgggc acagggaacc agtaaaatcc cggcatcagc
     3061 aggaatcacc ggtaatactt ttttcgcaat atgaagtccc tgacccacac aaccatactg
     3121 cccttttttc aggtctgctt ttggatggtt cagcagactc atattctgaa catcatgaag
     3181 gcagtgatct gctggaatga tttcattgta tttgcaggga atacctccgg gggtttgtgt
     3241 attcctgcgt gccagctgca taatacgtgg atcaggacga tcataagaat ccggcagggg
     3301 cagcccttca ccataagatg aagcattgga ctgaccggct ataacaatca cgtaatacca
     3361 ggaaggggag ctggaagccg tttcatttcc tgccggacgg gtatgtgttt cagcattatc
     3421 ccagtctgac ggttcagtag taccggcaac atcagcactt aacggaatga cagcaccaat
     3481 acccgctccg gtaatttcca cgttcggatc accggatact ggccttaccc acaggcaatc
     3541 ccgtgtatta aaactgatga gacagtcagc aaatgtcatt cctcctttcg tttcagccgg
     3601 aggaaaggga gcctcaagaa atgccgctgt accaccggaa actttaacgg taaagtttcc
     3661 gggcattgat gatagttgaa cccatttgga cgtcataata ttcatcctgt tcctgaataa
     3721 acagaattat catatccaca gaccgataaa cgccattttg cgttaattca gtaaaggccc
     3781 aacaaaggga gcgtcttatt tttccgaaat gctcgcagga taataacgat aaagggttga
     3841 aaggccgata tcaaaaataa gtgccacctg tcttcggctg acacccccgg ccagtaaacg
     3901 cccgacctgc tcgtattg
//
LOCUS       DAZGSP010000217         3708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZGSP010000217 DAZGSP010000000
VERSION     DAZGSP010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.219"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            137..322
                     /locus_tag="ACHK74_005100"
     CDS             137..322
                     /locus_tag="ACHK74_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223750.1"
                     /translation="MFISCVPSKATPLILRAFASLLAATAVPFDGRAFAALFTVSRKP
                     RFQGCRISALKGLSTSF"
     gene            336..896
                     /locus_tag="ACHK74_005101"
     CDS             336..896
                     /locus_tag="ACHK74_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0223751.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYYPASVSEK"
     gene            996..3035
                     /locus_tag="ACHK74_005102"
     CDS             996..3035
                     /locus_tag="ACHK74_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP0223752.1"
                     /translation="MTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMTFAD
                     CLISFNTRDCLWVRPVSGDPSVEITGAGIGAVIPLSADVAGTAEPSDWDNAETHTRPS
                     GNETASSSPSWYYVVVLAGQSNGMAYGEGLPLPETYDRPEPRIMQLARRSTVTPGGKA
                     CQYNDIILADHCLHDVQDMSGKNHPKADVAKGQYGTVGQGLHIAKKLLPFIPADAGIL
                     LVPCCRGGSAFTAGADGTYSDSTGASEDSARWGVDKPLYKDLISRTKAALAKNPKNRL
                     LAVVWMQGEFDIDAKPTEHSALFLAMVEKFRADLAEQAEQCTGGSAAGVPWICGDTTY
                     FWKQKNEQAYQAIYDGYKNKTDKNIHFVPLMTDENGANVPTNNPAEDPDIESIGYYGS
                     TWRNSAATWTSADRASHFSSWARRGIISDRLATAVLTHAGRTTVKADVPSSETEAPVP
                     SPSETEAVTTTLLSYRVSESEGNLKAQGWEPAGGKAEIISDAGGTGGKAMKLTKETGK
                     SSWYLDHDAGTGAELLKNGGLISCRFKVPGDLVANQYVMALYWPVSSLPQGVTLTGDA
                     GNNLLASFYIQTDAKDLNVMYHNAKVATNNQKLGTFGAFDNEWHTLAFRFAGNNSLQV
                     IPVIDGQDGAAFTLTQSPVGTFPVDKLRVTDITKNATYPVLIDSIVVEVKKAVTE"
     gene            3182..3364
                     /locus_tag="ACHK74_005103"
     CDS             3182..3364
                     /locus_tag="ACHK74_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0223753.1"
                     /translation="MTFVHTMLLYFCAVVSALYLVSGGYKVIRNYIRRKIDDAAAEKL
                     SKTAQAPSSPNDPTPL"
     gene            3400..3645
                     /locus_tag="ACHK74_005104"
     CDS             3400..3645
                     /locus_tag="ACHK74_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP0223754.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGHSPAENSTENEPQAVEQPEPVQPGTDGTMM"
BASE COUNT          977 a          854 c          990 g          887 t
ORIGIN      
        1 agtcttaaaa ccggtaatca catttcctga tgacagggag taattatatt ccagtccgcc
       61 gcttaatgcc tgcgctccca gtcttacccc tgtaacataa cgctgattgg ccgttgattc
      121 cggcattcct gacgtaatgt ttatatcctg cgtgccgtca aaggcgacac cattgattct
      181 gcgggcattt gccagcctgc ttgctgcaac agccgtaccg tttgatggca gggcattcgc
      241 ggccctgttt acagtatccc gtaaaccgag gtttcaggga tgccgtatat ctgctttaaa
      301 aggcctttcc acctcttttt aaggaaggta aatacatgct gattggctat atacgcgtat
      361 caacaaatga ccagaacaca ggtttacaaa gaaatgcgct ggaatgtgca ggatgtgagc
      421 agatttttga agacaaaatg agtggtaaaa cgacacagcg gccgggactg aagcgggcac
      481 tgaagaaact aaaacccggt gacacgctga tggtctggaa actggacagg ctggggcgca
      541 gtatgcgcca ccttgttgcg ctgacagatg aattacagca acaggggata aattttcgta
      601 gtcttactga caacattgat acgtcaaccc ccatggggcg cttctttttt cacataatgg
      661 gtgcgctggc agaaatggag cgtgaattaa ttgtcgaacg gacacgggct ggtcttgcag
      721 ctgcccgtga aaggggacga accggcggca gaccccgccg tctcacgcag gaacaatacg
      781 agcaggtcgg gcgtttactg gccgggggtg tcagtcgaag acaggtggca cttatttttg
      841 atatcggtct ttcaaccctt tatcgttatt atcctgcgag cgtttcggaa aaataagacg
      901 ctctctttgt tgggccttta ctgaattaac gcaaaatggc gtttatcggt ctgtggatat
      961 gataattcag tttattcagg aacaggctga ttattatgac gtccaaatgg gttcaactat
     1021 catcaatgcc cggaaacttt actgttaaag tttccggtgg tacagcggca tttcttgagg
     1081 ctccctttcc tccggccgaa acaaaaggag gaatgacatt tgctgactgt ctcatcagtt
     1141 ttaatacacg ggattgcctg tgggtaaggc cagtatccgg tgatccgagt gtggaaatta
     1201 ccggagcggg tattggtgcg gtcattccgt taagtgctga tgttgccggt actgctgaac
     1261 cgtcagactg ggataatgct gaaacacata cccgtccgtc aggaaatgaa actgcttcca
     1321 gctccccttc ctggtattac gtggtggttc ttgctggtca gtcaaacggt atggcttacg
     1381 gtgaaggtct gccactgccg gagacatatg accgccccga accgcgtatt atgcagttag
     1441 cccgtcgcag tacagtcacg ccgggaggta aagcctgtca gtataacgac atcattctgg
     1501 ccgaccactg tctgcatgat gtgcaggata tgagtggaaa aaatcacccg aaagcggatg
     1561 tggctaaagg gcaatatggc accgtgggtc aggggcttca cattgccaaa aaactgctgc
     1621 cgtttattcc ggcagatgcg gggattctgc tggttccctg ctgccgtggt ggttctgcat
     1681 tcacggcggg tgctgacggc acgtacagtg acagcacggg tgcttcagag gactcagccc
     1741 gctggggtgt ggataagccg ctgtataagg acttaatcag ccggacaaaa gcggcactgg
     1801 cgaagaaccc gaaaaaccgc ctgcttgctg tggtgtggat gcagggcgag tttgatattg
     1861 atgcgaagcc gacggagcat tctgcgctgt ttctggcgat ggtggaaaaa ttccgcgcag
     1921 acctggctga acaggcggaa cagtgtaccg gtggcagtgc tgctggcgtt ccgtggattt
     1981 gtggtgatac cacgtatttc tggaaacaga aaaacgaaca ggcataccag gcaatctatg
     2041 acggctacaa aaataaaaca gataaaaaca ttcatttcgt cccattaatg acagatgaaa
     2101 atggcgcgaa tgtacccacc aacaatccgg cagaagaccc ggatattgaa tccattggtt
     2161 attacggctc aacgtggcgt aacagtgccg ccacctggac atctgcggac cgggccagcc
     2221 atttcagttc atgggcacgt cgtgggatta tttccgaccg tctggccacg gcagttctga
     2281 ctcatgcggg gcgaaccaca gtaaaagccg atgttccatc ttctgaaact gaagcaccag
     2341 tgccgtcacc ttcagaaact gaagcagtaa ccacaacact gctgtcttac cgtgtcagtg
     2401 agtcagaagg aaatctgaaa gcccagggct gggagccggc tggcggtaaa gctgaaatta
     2461 tcagtgatgc aggaggcaca ggtggtaagg caatgaaact gaccaaggaa acaggtaaat
     2521 catcctggta tctggatcat gatgccggta ctggtgctga actgctgaaa aatggtggtt
     2581 taatcagttg tcgttttaaa gtaccgggcg acctggtggc aaaccagtat gtcatggcgc
     2641 tttactggcc ggtttcctct ctgccgcagg gtgtcaccct gacaggggat gcagggaata
     2701 atctgctggc atcgttctac atccagacag acgcaaaaga cctgaatgtg atgtaccaca
     2761 atgcaaaagt ggcaacaaat aaccagaaac tgggaacctt tggtgcattt gataatgaat
     2821 ggcatacgct ggccttccgt tttgcaggaa ataacagcct tcaggttatt ccggttattg
     2881 atggtcagga tggtgccgcg tttaccctga cgcagtcacc agtaggaact ttcccggtgg
     2941 acaaactccg cgtaacggat atcacaaaaa atgcaactta cccggtgctg attgacagca
     3001 ttgtggtgga agtgaaaaaa gcggtaacag aataaaaaaa tcccgccggt catgatatgg
     3061 ctggcgggag ctacctaacc cataaaggaa aataaaaata ctcccgaagg agtggttatc
     3121 agtcgcttcg atgtttttac ccgataacga attatgtagt caatatcaaa ggtaaggtta
     3181 tatgacattc gttcatacaa tgctgcttta tttctgcgca gtggtcagtg cgttatatct
     3241 ggtgagcggc ggttataagg tcatcagaaa ttatattcgc cgtaaaattg atgatgcggc
     3301 agctgaaaaa ctcagtaaaa cagcacaggc accgtcatcc ccaaacgacc cgaccccgct
     3361 ctgataacgg ggcaatatac ctgcaaaagg agaatattca tgccagagat taaaggcacg
     3421 gttactgaag agcttgtcaa acaggcactt tattctgaag aagtgaaccg cgtgctgaag
     3481 gcgcaggttc gcaaggattt tgaggcacaa atcgacgcat atgttgatga agtgctggcc
     3541 cgaatggttg gccattctcc ggctgaaaac agcacggaga atgagcctca agccgtagag
     3601 caaccagagc cggttcagcc cggaactgac ggtactatga tgtaacaata ccgaccggca
     3661 ggtttccctg ccgggtgaat ttgttaatgc acgttttgtg tactttcc
//
LOCUS       DAZGSP010000218         3700 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZGSP010000218 DAZGSP010000000
VERSION     DAZGSP010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.220"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            109..345
                     /gene="ycgZ"
                     /locus_tag="ACHK74_005105"
     CDS             109..345
                     /gene="ycgZ"
                     /locus_tag="ACHK74_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFP0223755.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            388..660
                     /locus_tag="ACHK74_005106"
     CDS             388..660
                     /locus_tag="ACHK74_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFP0223756.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            689..955
                     /gene="ariR"
                     /locus_tag="ACHK74_005107"
                     /gene_synonym="ymgB"
     CDS             689..955
                     /gene="ariR"
                     /locus_tag="ACHK74_005107"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFP0223757.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            1068..1316
                     /gene="ymgC"
                     /locus_tag="ACHK74_005108"
     CDS             1068..1316
                     /gene="ymgC"
                     /locus_tag="ACHK74_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFP0223758.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            1648..3171
                     /locus_tag="ACHK74_005109"
     CDS             1648..3171
                     /locus_tag="ACHK74_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0223759.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            3303..3521
                     /gene="ymgF"
                     /locus_tag="ACHK74_005110"
     CDS             3303..3521
                     /gene="ymgF"
                     /locus_tag="ACHK74_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFP0223760.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
BASE COUNT         1073 a          711 c          782 g         1134 t
ORIGIN      
        1 tggcactaat tgcaaaaaat taatttatca ttctgtacac atatttcgta caagtttgct
       61 attgttacct cacttaacat tgattaacat ttttaacaga ggcgtagcat gcatcaaaat
      121 tcagtgactt tagattctgc gggagcaatc actcgttact ttgcaaaagc taacttgcct
      181 actcagcagg aaactctcgg ggaaattgtg actgagattt tgaaagatgg acgtaatctg
      241 agtcgaaagt cgctttgtgc caaacttctt tgccgactgg aacacgcgac tggggaagag
      301 gaacagaaac attataatgc actaattggg ttgctttttg aataatgcaa cagcctgatt
      361 atttaacagg ttagctatga agtcgttatg aagacatctg ataatgaacg tataaaatat
      421 gaaattactg gccaggcggt gatccagata ctgcgcatga agataaattt ttcattgcag
      481 actcttatta agcaattact cgtaatgaaa tcggctgagg aagatgcttt ccgacgcgat
      541 ttaattgaca gcataattcg tgattttagc aatagtgatt caggagggcc aaaccgaaga
      601 acagcgacag ctgacaataa aagtatgttc aatggtaaga aaataaacag aatacattaa
      661 aatttcataa gtaagatgag aggttaccat gcttgaagat actacaattc ataatgcaat
      721 aactgataaa gcgttagcaa gttactttcg cagttcgggt aatttgttag aagaagagtc
      781 agcagtgtta gggcaggctg tcaccaattt aatgctttca ggcgataatg ttaataataa
      841 aaatattatc ttaagtctga tacactccct ggaaacaaca agtgatattc tcaaagctga
      901 tgtgattaga aaaacactgg aaatcgtgtt gcgatacaca gctgatgata tgtaacttct
      961 aattattaag tataagttta tagaaaactc attcatctta tttttgtctg tcgctttaga
     1021 ctatacacag cataatttta ttgggttaat atttctacga ggctgacatg aataattcaa
     1081 tcccagagag atttattttt caatgtgctt tgtttaaaaa tctcgagagg gaggtgttca
     1141 tgacgcatgg gtatgttgat agtcatatta ttgatcaggc tttacgcttg cgtttaaaag
     1201 atgaaaccag tgtgattctt tctgatctct atctgcaaat attgcagtac attgaaatgc
     1261 ataagactac actaacggat atcattatta atgacaggga atccgtgctc tcttagaact
     1321 tgagtaaggt tttagaataa acatcagctg tatcaccatg ctgatgcaaa gtgagcattt
     1381 caggcgttat gctttcttat tatgtccgca atatcaggtg tcaagaatgg agagttctcg
     1441 ctctccattc ttgacgcctg atatcccgcc taacttattt tgtactgcct acattatgct
     1501 caataattaa tgggttagat caagatggag aaaattttat cttcggcgtg gatatttctt
     1561 atgctctcta ccatgctctc tgtagatgcc agcgcgagcg aatgaatggc attttcatgt
     1621 attaaaccct ttggccggga caagcaaatg cgcaatacac tcatacccat cctcgttgcg
     1681 atatgcttgt ttattaccgg tgtcgctatt ttgaacatcc agctctggta ttccgcaaaa
     1741 gcagagtacc tggcgggagc gagatatgcc gccaacaata tcaatcatat acttgaagaa
     1801 gcgtcacaag cgactcaaac agcggttaac attgccggga aggaatgcaa cctcgaggag
     1861 caatatcagc ttggcactga agcagctctg aaacctcacc tgcgcacaat catcattctc
     1921 aaacagggaa tagtctggtg tacatccctg cctgggaata gggtcctgtt gtctcgtatt
     1981 cctgttttcc cggacagtaa tttactgttg gctccagcaa tcgacaccgt aaatagatta
     2041 cctatcctgc tctatcagaa ccaatttgca gatacgcgca ttttggttac gataagcgat
     2101 cagcatattc gtggggcact taatgtaccc ttgaaagggg taaggtatgt attacgcgtg
     2161 gcggatgaca ttattggacc aacgggtgat gtgatgacgc ttaatggaca ttatccctat
     2221 accgagaagg ttcactccac aaaatatcat ttcactatta tctttaaccc gccgccactc
     2281 tttagcttct acagacttat cgataaaggc tttgggattt taatatttat tctgttaatc
     2341 gcctgcgccg ctgccttcct gcttgataga tatttcaata aaagcgcaac gcctgaagag
     2401 atcctgcgac gggcaataaa taatggggag atcgtccctt tttaccaacc tgtggtaaat
     2461 ggtcgggaag ggacattgcg gggagttgag gtgttagccc gctggaaaca acctcacggt
     2521 ggatatatat cacccgcggc atttattcca cttgctgaaa aatccggatt aatcgttccg
     2581 cttacgcaaa gcctgatgaa tcaggttgcc agacagatga acgctatcgc gagtaaattg
     2641 ccggaaggtt ttcatattgg aattaatttt agcgcttcgc atattatttc gccgacgttt
     2701 gtcgacgagt gtttaaattt ccgtgacagt tttacccgcc gcgatttaaa ccttgttctg
     2761 gaagtcaccg agcgtgagcc attgaatgtt gatgaaagtc tggttcagcg gttgaacatt
     2821 ctgcatgaaa atggttttgt catcgcgctg gatgatttcg gtactggcta ctcagggctt
     2881 tcttatcttc atgacctgca tattgattat atcaaaattg atcacagttt cgttggccgc
     2941 gtaaacgcag acccagaatc aacccgaatt ctggattgtg tattggatct ggcgcgtaaa
     3001 ctttcgatca gtatcgtcgc tgaaggtgtc gaaacgaaag aacaacttga ctatctgaac
     3061 caaaataata tcacatttca gcagggttat tatttctata aacctgttac atacatcgac
     3121 ctggtcaaaa ttatcctttc taaaccgaag gtgaaggttg tggttgagtg aaaattgatc
     3181 agtaaggcca tagtgcggtg taattataga cagctaatta gctcgttgcc tcttgttact
     3241 attgttcatt attttgtttg ctataattgt ttgaaagttt tgacaggatt gccattagta
     3301 gcatgaacaa tagtaataat ctggattatt tcactctcta tatcatcttt tccattgcat
     3361 ttatgctgat caccctcctg gtcatcctta ttgcaaaacc cagtaccggg ctgggagaag
     3421 tgcttgtgac gataaatttg cttaatgccc ttgtttggct ggcgatcaat ctggttaatc
     3481 gattaaggga aagactcgtc agccacaggg atcagcaata atctttcagt ttctcactgt
     3541 cagtatgcgg ctgaatgggt tgctggcagt gaatgcctgg atcattgaag taaaggaatt
     3601 attgcgcaaa tagttgtcaa ccctggtgtt atcacagttg tttttatatg tcattgaaat
     3661 aatttttatt gcaactatca atgattgtga tgtcgaagag
//
LOCUS       DAZGSP010000219         3685 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZGSP010000219 DAZGSP010000000
VERSION     DAZGSP010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.221"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            158..388
                     /gene="vapB"
                     /locus_tag="ACHK74_005111"
     CDS             158..388
                     /gene="vapB"
                     /locus_tag="ACHK74_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HFP0223761.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLELEKADPDFMTEREDVVSDEGRVNL"
     gene            385..801
                     /locus_tag="ACHK74_005112"
     CDS             385..801
                     /locus_tag="ACHK74_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFP0223762.1"
                     /translation="MNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRAAVDATTEIKVALRLAGTPIGPN
                     DTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR"
     gene            876..2441
                     /locus_tag="ACHK74_005113"
     CDS             876..2441
                     /locus_tag="ACHK74_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0223763.1"
                     /translation="MPLITRLMLQNFKKFPELDLRFSNDRNILVGDNESGKSTILLAL
                     DLVLSDSRHRVEALGVESLLSQSAVRHFQEGERRADQLPVLTADVFLSNGGEPDLNGR
                     QNLAGIDADGLRMRIAPMMEEYGQDIHHVLQQDPDNFPYEYYSVQFSTFSGGHFAGFR
                     RYLRHLFLDSARIDNEHAAQEYTRTVYSVNVPVADRYRLENSYRQQKMHFCARHLSAI
                     NDTLKTYQFGVRSGAKSGLEANLDITEDGISIRHRGKGRQCFIKTEFALQRHQQQGGE
                     IHALLLEEPENHLSHVSMKRLVNQLATERQTQVFIATHSSHISSRLDLRKAILLGATR
                     PVLMNELSAETAAFFMKAPDNNVLEFALARRVLLVEGDAEFILIEAFYHRLYGRAPED
                     DGVHIIAIGGTSFRRYLELARLLENRVAALRDNDGNYQQNCDERYADVLCSRSRVFAD
                     HDNSRSTFEICLYQDNADLCDALFRGTRRTLTVQDYMLANKAEAAFQLLQLHAEKLTV
                     PDYIQEALAWIRE"
     gene            2426..3448
                     /locus_tag="ACHK74_005114"
     CDS             2426..3448
                     /locus_tag="ACHK74_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HFP0223764.1"
                     /translation="MDKRVIFAVAGSGKTTLLIRRLREDRRTLILTFTVNNEAHLRAQ
                     IIRRFGYIPYGIRVMTWFEFLHGFCFRPFLQEQLSSRGLSFNQPPSRIPRTNIRHYQD
                     PAGRLYHRRLAHLLTARGLLPDIRTRLARYYDELFVDEVQDFAGHDFNFLLELCRAEI
                     SVLCCGDFYQHTFDTSRDGNVNATLHEDITRYEARFRAAGIMVDCETLSRTWRCSATV
                     CEFITGQLNIRISAHGTHTTQIEIVTDEARSAALHADNTMIKLFYREHHRYGCHSMNW
                     GGSKGLDHFQDVCIVMGANHWMRLIQQKLAALPPSSRNRLYVACSRARGNIYFIPESH
                     LRRFRN"
BASE COUNT          881 a          977 c         1004 g          823 t
ORIGIN      
        1 cagaaaccgg atctgcacgg ggaaagagag aaaataacat aggctgaaca tatccatatg
       61 cctgacatct gcctatacta aacatatgca cacacccggt gcaatgaaca tcattccgga
      121 tatgcatcac atatccacac ttacccggga gagaatcatg agaaccgtat ccatttttaa
      181 aaatggcaac aaccgtgcta tccgccttcc acgcgatctg gattttgagg gggtgagcga
      241 gctggagatc gtccgggaag gggacagcat cattctgcgc cccgtccggc cgacctgggg
      301 ctcattcctg gagctagaaa aggccgatcc ggactttatg acggagcgcg aggacgttgt
      361 cagcgacgaa ggacgagtta acctgtgaac aagatctata tgctcgacac gaacatctgc
      421 tcgttcatca tgcgcgagca acctgaagca ttgcttaagc acctggagca gtcggtgctg
      481 cgcggtcacc gtatcgtggt ctcggccatc acctactccg agatgcgctt cggtgccacc
      541 ggtccgaagg cctcgccacg ccacgtgcaa ctggttgacg cgttctgtga gaggctcgat
      601 gccgtcctgc cgtgggaccg cgccgcggtg gatgcgacca cggagattaa ggtggcactg
      661 cgtctcgccg gcacgccgat cggtccgaac gacacggcga tagccggaca cgccatcgct
      721 gcctgtgcca ttctggtgac gaataatgtg agagagtttg agcgggtacc gggtcttgtg
      781 ctggaagact gggtaagata acgctgtaaa acaaaacagg cggtcaggcg cggccggccg
      841 ctctttcagg cagcggtata taaaggaata aaaaaatgcc actgatcacg cggcttatgc
      901 tgcaaaactt caaaaagttt ccggaactgg acctgcgctt cagcaacgat cgtaacattc
      961 tggttgggga caatgagtcc gggaaaagca ctattctgct ggctcttgac cttgtgctga
     1021 gtgacagcag gcatcgggtt gaggcgctgg gggtggaatc gctcctgtca cagtcagcag
     1081 tcaggcattt tcaggaaggt gagcgccgtg ccgatcagtt acccgtgctg accgctgacg
     1141 ttttcctgag taatggggga gagcctgacc tgaacggcag gcaaaatctg gcgggtatcg
     1201 atgcggatgg tctcagaatg cgcattgctc ccatgatgga ggaatacggt caggatatac
     1261 accatgtttt gcagcaggat cctgataatt ttccctatga atactattcc gtgcagttca
     1321 gcacattctc cggtggtcat ttcgcaggct tcagacgcta tctgcgccat ctgttcttag
     1381 acagtgcacg cattgataac gagcatgccg cacaggagta tacgcgcaca gtttacagcg
     1441 tcaatgtgcc ggtggccgat cggtaccggc ttgaaaacag ttaccggcaa cagaaaatgc
     1501 acttctgcgc ccggcatctg tccgccataa acgacacgct gaaaacgtat cagtttggcg
     1561 tgcgttccgg cgctaaatca ggtcttgagg cgaacctgga cattactgag gacggtattt
     1621 ccattcgtca ccgcggaaaa ggaagacagt gtttcatcaa aacggagttt gcgctgcagc
     1681 ggcatcagca gcagggggga gagatccacg cgctgttgct tgaggagccg gagaatcatc
     1741 tcagccatgt cagtatgaag aggctggtta atcagcttgc cacggaacgg cagacgcagg
     1801 tgtttattgc cacacacagt agccatatct cctcccgcct cgacctacgt aaggctattc
     1861 tcctcggagc tacccggccg gtactgatga atgaactttc cgctgagact gccgccttct
     1921 tcatgaaagc accggataat aacgtgctgg aatttgcgct ggccagacgc gtgctgctcg
     1981 tggaggggga tgctgagttt attctgatcg aggcctttta tcaccgcctg tatggcaggg
     2041 caccggagga tgacggggtt cacatcatcg ctatcggcgg aacgagtttc cgtcgttatc
     2101 ttgaactggc ccgtcttctg gaaaatcgtg tcgcggccct tcgcgacaat gacggcaact
     2161 atcagcaaaa ttgcgatgaa cgctatgctg acgtgctttg ttcccgctcc cgggtatttg
     2221 ctgaccacga caacagccgc tccacatttg aaatctgcct gtatcaggat aatgccgacc
     2281 tgtgtgatgc gcttttccgc ggaacccgcc ggacactcac ggttcaggat tacatgctgg
     2341 ccaataaggc agaagcagca ttccagctgt tacagttgca cgccgaaaag ctgaccgttc
     2401 ctgattatat ccaggaggca ctggcatgga taagagagtg atatttgcgg ttgccggctc
     2461 gggtaaaacc acgctgctga tacgtcggtt aagagaggac cggcggacgc tgatcctgac
     2521 cttcaccgta aataatgaag cacatctgcg ggcgcagatt atcagacgct ttggctacat
     2581 accgtacggg atccgtgtga tgacctggtt tgagtttctg cacggtttct gttttcgccc
     2641 tttcctgcag gagcaactgt catcgcgtgg tctgagcttc aaccagcccc cttccagaat
     2701 accgcgcacg aatatccggc attatcagga tcccgccgga cggctgtatc accggcggct
     2761 cgcacacctg ctgacagccc gggggttgct gccagatatc cgtacccgac ttgcccgtta
     2821 ctacgatgag ttgttcgttg atgaagtcca ggactttgcc ggccatgact ttaattttct
     2881 gctggagcta tgccgcgcgg aaatctcagt gctgtgctgc ggggattttt atcagcatac
     2941 ctttgacaca agccgcgacg gtaacgtcaa tgctacgctg catgaagaca taacccgata
     3001 cgaggctcgc ttcagggctg cgggtatcat ggtggattgt gagacgctca gccggacatg
     3061 gcgctgttca gctacagtgt gtgagttcat taccggacaa ctgaacattc gcatttcggc
     3121 tcatggaaca cataccaccc agattgaaat cgtcacggat gaagcgcgtt cagcggccct
     3181 gcacgcggac aacacgatga tcaagctctt ctatcgcgaa catcaccgtt acggatgcca
     3241 ctcaatgaac tgggggggca gtaaaggact cgaccacttt caggacgtct gcatcgtgat
     3301 gggggccaac cactggatgc gcctcatcca acagaagctg gcagcacttc caccatcatc
     3361 ccgtaacagg ctatacgtgg cctgctcacg ggcacggggc aacatctatt ttattccgga
     3421 aagccatctt cggagattca gaaactaagt cagcggcttc tcaactgggc acaagccggt
     3481 aaaaagtagt caacgacaaa acttcaggcc gaaaaatgag gctccggttt cgccggaaac
     3541 cggacctgcg tggagggaaa cccgcagatg atatgatatt tatcattaat cgcataataa
     3601 atcctggcct gatatttaca ggcagagaaa taaatcattc gatatatgat tttatcgtca
     3661 tcagccatta accccccagg aagcg
//
LOCUS       DAZGSP010000220         3536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZGSP010000220 DAZGSP010000000
VERSION     DAZGSP010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.222"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1109)
                     /locus_tag="ACHK74_005115"
     CDS             complement(<1..1109)
                     /locus_tag="ACHK74_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFP0223765.1"
                     /translation="MRKLSMVVCSVVSALMLCEATQAAEIYNKDGNKLDFYGRVKARH
                     YISDGSMDGDRTFARLGFKGETQINESLTGFGQWEYQFLGFKTESEVRNDKNRLAFAG
                     IRHNILGSLDYGRNYGVAYDVGALTDVLPDFGGDSWTYSDNYMTGRTTGVLTWRNTDF
                     FGLVDGLTFSAQYQGKNDRDNLLEANGDGYGFSVSYDYEGFGIVAAYTNADRTSLQEK
                     EGRKRFMTSKDSEGKEVITPRWIAGGKHAEFAGVGVKYNDNDLYLAADYSETRNMTPF
                     GSAGIADKARNIEVVAQYMFDFGLRPSLAYVQSKGMDVKGGIVNYGDQSLVEYIDVGA
                     NYFLNKNMSLLIDYKINLIDESEFTKKAGVATDNIL"
     gene            1785..1993
                     /locus_tag="ACHK74_005116"
                     /pseudo
     CDS             1785..1993
                     /locus_tag="ACHK74_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1980..2169
                     /gene="tnpB"
                     /locus_tag="ACHK74_005117"
                     /pseudo
     CDS             1980..2169
                     /gene="tnpB"
                     /locus_tag="ACHK74_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(2172..2360)
                     /locus_tag="ACHK74_005118"
     CDS             complement(2172..2360)
                     /locus_tag="ACHK74_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFP0223766.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDSEMTFAECEKLFAPAKEP
                     GRYIRVRVEDFRCEVVKA"
     gene            2895..3036
                     /locus_tag="ACHK74_005119"
                     /pseudo
     CDS             2895..3036
                     /locus_tag="ACHK74_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            3259..>3536
                     /locus_tag="ACHK74_005120"
     CDS             3259..>3536
                     /locus_tag="ACHK74_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223767.1"
                     /translation="MIVCYVILVNRPFETEIAGKKIFFQKNDVLLTDNISQSFFLRHP
                     GNVTELRIDECIVRKYMESADRIQHSDSAVSVPFIKTTFSHIALLHNV"
BASE COUNT          978 a          831 c          691 g         1036 t
ORIGIN      
        1 agtatattat cggttgcaac tccagccttt ttggtaaatt cgctttcatc aataaggtta
       61 attttgtaat caataagaag tgacatgttt ttgttcagaa agtagtttgc gccaacatca
      121 atatactcaa caagagactg atcgccatag tttactattc cacctttaac atccatccct
      181 ttggactgaa cgtaagccag tgaaggacgc aggccgaaat caaacatata ctgagccaca
      241 acttcaatat tccgtgcttt atctgcgatg cctgcgctac caaatggagt catatttcgg
      301 gtttcagagt agtcagctgc cagatacagg tcattatcat tatacttgac cccaacacct
      361 gcaaactccg catgttttcc cccggcaatc cagcgaggcg taataacttc ttttccttct
      421 gaatctttcg atgtcatgaa acgtttacgg ccttcttttt cctgcagact ggttctgtcg
      481 gcattagtgt aagcggcaac gattccaaaa ccttcatagt catagcttac ggagaaacca
      541 tatccatcac cattagcttc aagtaaatta tcacggtcat ttttaccctg atattgagca
      601 gaaaaagtaa gtccatcaac aagcccgaag aaatctgtat tgcgccaggt cagaactccg
      661 gttgttctgc cggtcatata gttatcagag taagtccagc tgtcaccacc aaagtccgga
      721 agtacgtcag tcagagcccc cacatcatag gcaacaccat agtttcgccc ataatcaagt
      781 gaaccgagta tattgtggcg aatacctgca aatgccagac gatttttatc gttgcgtact
      841 tcactttctg tcttaaaccc caggaactgg tattcccact gaccaaagcc agtcaggctc
      901 tcattaattt gtgtttcgcc tttgaaacca agacgtgcaa atgttctgtc gccatccata
      961 gaaccatcag agatgtaatg acgggcttta acgcgaccat aaaaatccag tttattaccg
     1021 tctttattat agatttctgc tgcctgtgtt gcctcacaca gcattaatgc agaaacgaca
     1081 gagcacacaa ccatacttaa ttttcgcata aaaaaatccc ctgtgcacat ttttagtatg
     1141 gttttaaaaa ttaaactatg gcgaaaaaat tatcactatg caaagatgta acaaaatatt
     1201 gctcagtgat ctatattgtt gtttttattt gaggttttat attttctagt gtatttgact
     1261 tttctttctc aagtgttatt tcttgacctt tatcacagaa ccatctccct ggatatcccc
     1321 atcacattgc atgaaaagca agttttacca atcttgattt tcagtgttaa ataagattaa
     1381 taactgtaat tctgcaaaaa aaagaacacc atatgtagta tgactacgta ttccactctg
     1441 ttcgttcacg gactggcatc gaagtctgag gtgtactggc aatagcggat acagtcattt
     1501 gttctttttt taagcattca tccgatgata cttttcctga atgctaccgg agtggtattc
     1561 ccctgccgtg agggagggcg ctgacggtta taaaaatcct gatatatacc cattcccgta
     1621 tgacagcgat ggcgtcacat ctctgttcta ccatcttcac agaaatattt cgttcattag
     1681 cattccggta ttcgtataac caccttcggt ggagaaagaa taaacaatcc ctggaatgcg
     1741 atgagcgctt gaccagaaaa gcatcatatt gcttcatggc gcatcagcga ccacaggatg
     1801 ccgcaaaagc cgaaaaacgg accagagcgc ctgcaatcat tcctcgcctc agtatctgtt
     1861 gcccatggag tggccgataa cagatcattg tcggatgagc cttatcccct tccttcccgg
     1921 tggtatccgg atgtgctgcc agccttctca gttaaccgat gttttccggg ctttttgcca
     1981 tactgaggac tgataacgta ttcctggtcg taaaacccgt caatatgcgc cggggcatcg
     2041 acaaaactga cacagtacgt ccggaaagaa cttaacgctg catggcacga caatgctgcc
     2101 tttgtcttta ccaacaaggc tcgttcccgc ataaaagttc tacgccggga taaacatggc
     2161 gtatggctgc gtcaggcctt cactacttca caccggaagt cttccaccct gacacgaata
     2221 taccgtccgg gctctttcgc aggtgcaaac agtttttcac attcggcgaa tgtcatttct
     2281 gaatcctggt aacgcaacca ctgaaccggc atattaccgg gtttgattat tgtcgcagtt
     2341 attttatatc gtttccccat atcgcctctc atcctgacaa caaaattacc ggctgagcca
     2401 tcactccggt aaagcatgcg aaaaaaagtg agcgtctcag caatggctca tgcgatgatt
     2461 ctgtacgggc accgatggca cctcagaata caacaaagat atcagagtaa catgtctctc
     2521 tcgttatttt tcagagttct tgacaccctc cttctgccac attgtcaacg ccagtggaga
     2581 cttctgctgt cagttttttc atccatacag agtgacatta attatcccga acaaaacgaa
     2641 atatcctgaa tatatttatt ttctcctggt gccggagaca tgatgatgta caggctcagg
     2701 tgcggtgtga atgtaaaatg gtatgttaat tattcatttc tgaatgcaaa ctgaatgcaa
     2761 acttattccg aattgaattc tcctgacacc acatgataaa aatgaggata taataaaata
     2821 cttctcttct ggatgtgcca ttacctgccg ttgctgtaat acggcgggta ttttttactg
     2881 acatatcggg aagccagcag acgttcgtag ctacatcccc gaaagtggca cactgcaaac
     2941 tataatacga gtaaaacatc gatcaccgta gctgatgtgt tcaacgatcg tgcactagcg
     3001 ttctgcgatt ctcagggact gcctgttctg agaatcggcg agtccgcgat cggatacgat
     3061 gcgatttcgc agtgactcag ggattgcatc tgaaaaccct ccggctatgt cggtaaatat
     3121 ttatttctat gaaccgtacg attcagatag cattcgcccg gccccgtaag aataatctcc
     3181 ggacaaataa gatttttcct gaaaaagcgt ctagtctttt gatatcgttg tttttatgct
     3241 aatcagggag tatgtgtgat gattgtctgt tacgttattc ttgttaaccg tccgtttgaa
     3301 acagagattg ccgggaaaaa gatatttttt cagaaaaatg acgtcctgtt gacagataac
     3361 atcagccagt cctttttcct gcgccatccc ggtaatgtta ctgagctccg gattgatgaa
     3421 tgtattgtca gaaaatatat ggaaagtgcc gacaggatcc agcactctga tagtgctgtt
     3481 tctgttccgt tcattaaaac aaccttctct cacatcgctc tgttacataa tgtcat
//
LOCUS       DAZGSP010000221         3516 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZGSP010000221 DAZGSP010000000
VERSION     DAZGSP010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.223"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            171..386
                     /locus_tag="ACHK74_005121"
                     /pseudo
     CDS             171..386
                     /locus_tag="ACHK74_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            561..2531
                     /locus_tag="ACHK74_005122"
     CDS             561..2531
                     /locus_tag="ACHK74_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HFP0223768.1"
                     /translation="MYMNVIRTVICTLIILPVGLQAATSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVITNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGVRQLSDMAKSSGTYLDPALIKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGVSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVSITDSNKAGASLRLYQNNTTEPGNPTLTHGDNGLRDRKTAQNDV
                     QFWYQYSPADNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSNPVNIIVGSRYDR
                     YKSFNPRAGKLKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKGFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            2550..3341
                     /locus_tag="ACHK74_005123"
     CDS             2550..3341
                     /locus_tag="ACHK74_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFP0223769.1"
                     /translation="MMKNTGYILALCLTASEHALAHDVWITGKQAENNITAEIGYGHN
                     FPSKGTIPDRRNFFENPRLYNGKEIITLKPTSTDYVYKTESAGNGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGDELEIIPLS
                     DISRFGEDVKLKVLYKTSPLAGATLELDSVSYLKSSRHTHAAEHKHSAHKAELTFVSN
                     EDGIITVPSLHVGQWLAKVKNKKVFPDKSLCDETVDVATLSFSRN"
BASE COUNT         1040 a          803 c          827 g          846 t
ORIGIN      
        1 aaaaacaggt cagaccgacg ttacgaaaac ctgcaagatg agccaggcca gtaaggccga
       61 gtaagcgata aggtcggagc gcgcgaatgt ctcatcaagg ccagaagcaa taacttcatg
      121 aacaaaccgg atatacgaaa gtaaacctgt ccctatcata gttgctgtac tcttgcaata
      181 cgggggaaga ccatctcatc gcttacgaac aattcgtgaa aacgatgaag aaagactaca
      241 tcgcgtgcat gccgaaatct aatgtaagaa cggcattgca taatcttgca gtggagatcg
      301 ggtattacaa tgaaaaccat ccgcatagtg cgttaggtta tctcccacca agggaatatc
      361 aacgtcagcg aataacgtca ccttagatgc agtagttgtc aggaaatagt gactcaaggg
      421 caggcttacc atatacactc ttagtggttc gactgggtat tagaatagct attaacagat
      481 tagcactgaa cactgcgtaa tagtggttgc acaaacaaaa tgttatgtta taacattgct
      541 gtattgacat atattttcga atgtatatga acgtaatcag aactgtcatt tgtacattaa
      601 tcatacttcc ggtggggtta caggcagcga caagtcattc ttctatggct aaagatacaa
      661 tcaccgttgt cgcgacagga aatcagaaca cggtatttga aacgccgtcg atggtcagtg
      721 tcatcacgaa tgacacgcca tggagtcaga atgcggtcac atcagccggt atgctgaaag
      781 gtgttgcggg tctcagccag actggtgcag gacggaccaa tgggcagacc tttaatctac
      841 gcggctatga caaaagcggg gtacttgttc ttgttgacgg cgtccgtcaa ctcagtgaca
      901 tggcaaaaag cagtggcact tatctggatc cggcactcat caaacgcatc gaagttgtcc
      961 gcggaccaaa ctccagtctg tacggcagtg gcgggctggg gggggtggtg gacttcagaa
     1021 ctgccgatgc tgcagatttt cttccccccg gagagacaaa tggtgtaagt ctgtggggaa
     1081 atatcgccag tggtgaccac agcacaggct cggggctcac ctggttcggt aaaaccggaa
     1141 aaacagatgc gcttctttct gtcattatgc gtaaaagagg aaatatctat cagagtgatg
     1201 gtgagcgtgc acctaacaag gaaaaacctg cagccctgtt tgcgaaaggc tctgtcagta
     1261 taacagacag taacaaagca ggtgccagct tgcgtctcta ccagaataac accactgaac
     1321 cgggcaatcc cactctgaca catggtgaca acggcctgcg tgacagaaaa acagcacaaa
     1381 atgacgtgca gttctggtac cagtactccc ctgcggataa cagcctcatc aatgtaaagt
     1441 caacgttata tctcagtgat atcactatca agacaaacgg tcacaacaaa acagcagaat
     1501 ggcgaaacaa cagaacctcc ggtgttaatg ttgtcaacag gagccatact ctgatttttc
     1561 cgggagccca tcagttaagt tatggcgccg aatattaccg tcagcagcag aagccggaag
     1621 gctctgccac actatatccg gaaggaaaca ttgactttac atcgttgtat ttccaggatg
     1681 aaatgacaat gaaaagcaac ccggttaaca ttatcgtcgg ttcccgttat gaccggtaca
     1741 agagcttcaa tccccgagcc ggaaaactga aagccgaacg cctgtccccc agggcggcga
     1801 tttcagtctc accaacagac tggctgatga tgtacggctc catatcctct gcattccgtg
     1861 cccccacaat ggcagaaatg tacagggatg atgtacattt ttaccgcaag ggtaaaccca
     1921 attactgggt acctaacctt aatctgaaac cagaaaataa cattacccgt gagattggcg
     1981 caggtattca actggacggc ctgcttacag acaatgaccg gctgcagttg aaaggcggat
     2041 atttcggaac ggatgccaga aactatattg ccacgcgcgt ggatatgaaa cggatgcgtt
     2101 cttattctta taatgtatcc cgggcccgta tctggggatg ggatatgcag ggtaattacc
     2161 agtctgatta ttttgactgg atgctttctt ataaccgcac ggaaagtatg gatgccagta
     2221 gcagggaatg gctgggctcc ggcaatcctg acacactcat cagtgacatc agtatacctg
     2281 ttggtcatgg aggagtttct gccggatggc gtgctgaact ttcggcagca gccacgcatg
     2341 tgaaaaaagg cgatccccat caggctggct atgccgtaca ctccttttca ctgtcttata
     2401 agcctgtaag tgttaaaggc tttgaggcat cagtgactct ggataatgcc ttcaacaagc
     2461 ttgccatgaa tggcaaaggt gtgccgcttt caggcagaac tgtcagtctt tatacccgtt
     2521 atcagtggta acctcgcagg aaataaatca tgatgaaaaa tacaggctat atcttagctc
     2581 tttgtctgac agcatcggag catgccctgg cccacgatgt ctggattacc ggtaaacagg
     2641 cagagaacaa cattaccgca gagattggtt atggtcataa tttcccctca aagggcacaa
     2701 ttcctgacag aaggaatttc tttgaaaatc cccggcttta taacgggaaa gagataataa
     2761 cgctgaagcc aacgtccacg gattatgtct ataaaactga gtctgcaggc aacggtaata
     2821 gctacgttct gtcaacgaaa atgaaaccgg gatactggtc gagaacctcg tcaggatgga
     2881 agcctgtcag tcgggagggc agaaatgatg tagcatactg tgagtttgtg actaaatatg
     2941 caaaatcttt tattcctggt gaacagcaga tgccagcaca actctatcag tctccaacag
     3001 gggatgagct tgaaatcatt ccattatccg atatcagtcg tttcggtgaa gatgtgaagc
     3061 tgaaagttct gtataaaaca tccccgcttg ccggagctac tctggagctt gactctgtca
     3121 gttatctgaa atcatcccgt catacccatg cagctgagca taaacattcg gctcataaag
     3181 cagaacttac ctttgtaagt aatgaggacg gtatcatcac tgtaccttcc cttcatgtcg
     3241 ggcaatggct ggctaaagtc aaaaataaga aagtatttcc ggacaagagt ctgtgtgatg
     3301 aaactgtcga tgtggcaacc ttaagcttct cccgaaatta ataatactat tcctgccctg
     3361 atttatgggc agggtattat caatacgaga atagcctacc gacatccgtg taaaaaacca
     3421 cataatgatt tttcatatcc atttccagat atacttatgt aaagatcacc tccaaaaaag
     3481 cgtaatagta ataaaaatcc ctgaagcacc ctgttc
//
LOCUS       DAZGSP010000222         3485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZGSP010000222 DAZGSP010000000
VERSION     DAZGSP010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.224"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2208
                     /gene="agn43"
                     /locus_tag="ACHK74_005124"
                     /gene_synonym="flu"
     CDS             <1..2208
                     /gene="agn43"
                     /locus_tag="ACHK74_005124"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFP0223770.1"
                     /translation="DVTQNTGGALVTSTAATVTGTNRLGAFSVVAGKADNVVLENGGR
                     LDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLADSGAAVSGTRSDGKAFS
                     IGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAGTTTLNNGAILTLSG
                     KTVNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLTQKAVNLNEGTLTLN
                     DSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIPDNATVQSVVDNLSH
                     AGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNADRLVIDGGRATGKT
                     ILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNRLQAGAFNYSLNRDSDE
                     SWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLG
                     HDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGIYGAAGHSSVDVKDDDA
                     SRAGTVRDDAGSLGGYLNLTHTSSGLWADIVALGTRHSMKASTDNNDFRARGWGWLGS
                     LETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFGYGSAQHVRAGFRLGSHN
                     DMTFGEGTSSRDTLRDSTKHGVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTF
                     SPSRNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            2329..>3485
                     /locus_tag="ACHK74_005125"
     CDS             2329..>3485
                     /locus_tag="ACHK74_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223771.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLNGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANG"
BASE COUNT          807 a          955 c          988 g          735 t
ORIGIN      
        1 gatgtcaccc agaacacggg cggtgcactg gttaccagta cagctgcaac cgtcaccggt
       61 acaaaccgcc tgggagcatt ctccgttgtg gcgggtaagg ctgataatgt cgtactggaa
      121 aatggcggac gtctggatgt gctgagcgga cacacagcca ctaatacccg tgtggatgat
      181 ggcggaacgc tggatgtccg caacggtggt gccgccacca ccgtatccat ggggaatggc
      241 ggtgtactgc tggccgattc cggtgccgct gtcagtggta cccggagcga cggaaaggca
      301 ttcagtatcg gaggcggtca ggcggatgcc ctgatgctgg aaaaaggcag ttcattcacg
      361 ctgaacgccg gtgatacggc cacggatacc acggtaaatg gcggactgtt caccgccagg
      421 ggcggcacac tggcgggcac caccacgctg aataacggcg ccatacttac cctttccggg
      481 aagacggtga acaacgatac cctgaccatc cgtgaaggcg atgcactcct gcagggaggc
      541 tctctcaccg gtaacggcag cgtggaaaaa tcaggaagtg gcacactcac tgtcagcaac
      601 accacactca cccagaaagc cgtcaacctg aatgaaggca cgctgacgct gaacgacagt
      661 accgtcacca cggatgtcat tgctcagcgc ggtacagccc tgaagctgac cggcagcact
      721 gtgctgaacg gtgccattga ccccacgaat gtcactctcg cctccggtgc cacctggaat
      781 atccccgata acgccacggt gcagtcggtg gtggataacc tcagccatgc cggacagatt
      841 catttcacct ccacccgcac agggaagttc gtaccggcaa ccctgaaagt gaaaaacctg
      901 aacggacaga atggcaccat cagcctgcgt gtacgcccgg atatggcaca gaacaatgct
      961 gacagactgg tcattgacgg tggcagggca accggaaaaa ccatcctgaa cctggtgaac
     1021 gctggcaaca gtgcgtcggg gctggcgacc agcggtaagg gtattcaggt ggtggaagcc
     1081 attaacggtg ccaccacgga ggaaggggcc tttgtccagg ggaacaggct gcaggccggt
     1141 gcctttaact actccctcaa ccgggacagt gatgagagct ggtatctgcg cagtgaaaat
     1201 gcttatcgtg cagaagtccc cctgtatgcc tccatgctga cacaggcaat ggactatgac
     1261 cggattctgg caggctcacg cagccatcag accggtgtaa acggtgaaaa taacagcgtc
     1321 cgtctcagca ttcagggcgg ccatctcggt cacgataaca acggcggtat tgcccgtggg
     1381 gccacgccgg aaagcagcgg cagctatggc ttcgtccgcc tggagggtga cctgctgaga
     1441 acagaggttg ccggtatgtc tgtgaccgca gggatatatg gtgctgcagg ccattcttcc
     1501 gttgatgtta aggatgatga cgcctcccgt gccggcacgg tccgggatga tgccggcagc
     1561 cttggcggat acctgaatct gacacataca tcctccggcc tgtgggctga cattgtggcc
     1621 ctgggaaccc gccacagcat gaaagcgtca acggacaata acgacttccg cgcccggggg
     1681 tggggctggc tgggttcgct ggaaaccggt ctgcccttca gtatcactga caatctgatg
     1741 ctggagccgc aactgcagta cacctggcag ggactctccc tggatgacgg ccaggataac
     1801 gccggttatg tgaagttcgg gtatggcagt gcacaacatg tgcgtgccgg tttccgtctg
     1861 ggcagccaca acgatatgac ctttggtgaa ggcacctcat cccgtgacac cctgcgcgac
     1921 agtacaaaac acggtgtgag tgaactgccg gtgaactggt gggtacaacc ttctgttatc
     1981 cgcaccttca gctcccgggg ggacatgagc atgggtacag ctgcagccgg cagtaacatg
     2041 acgttctcac cgtcccggaa tggcacgtca ctggacctgc aggccggact ggaagcccgt
     2101 gtccgggaaa atatcaccct gggcgttcag gccggttatg cccacagcgt cagcggcagc
     2161 agcgctgaag ggtataacgg tcaggccaca ctgaatgtga ccttctgaca gaaccatcgc
     2221 ctctctgtgg tcccggtcat catgaccggg acccggaccg gcgcaacgga tcttcaacgc
     2281 cacattcgct ggcattaaca ataacatgat attcatcacg gagtgactat gttacagata
     2341 gtcggcgcgc tgatcctgct gatcgcagga tttgccattc ttcgcctttt gttcagagca
     2401 ttaatcagca cggcttctgc gctggcaggg ctcatattgc tgtgtctgtt cggcccggcc
     2461 ttactggctg gctatatcac cgaacgcata acccggttgt tccatattcg ctggctggca
     2521 ggcgtatttc tgacgattgc cggaatgatc atcagcttca tgtggggact tgatggtaaa
     2581 catatcgcgc tggaggctca cacctttgac tctgtgaaat ttattctgac caccgctctc
     2641 gccggtggtc tgctggctgt tcccctgcag atcaaaaaca ttcagcagaa cgggatcaca
     2701 ccagaagata tcagcaagga aattaacggg tattactgct gtttttatac tgcctttttc
     2761 cttatggcgt gttctgcatg cgcaccattg atcgcgttac agtacgatat ttcaccgtca
     2821 ctgatgtggt ggggcgggtt gttgtactgg ctggctgcat tagtaacgct gctatgggcg
     2881 gccagccaga tccaggcgct gaaaaaactg acctgtgcca tcagccagac actggaagaa
     2941 caaccggtgc tcaacagtaa atcgtggctg accagtttgc aaaacgatta cagccttcct
     3001 gactcactga cggagcgcat ctggctgacg ctcatttctc aacggatttc ccggggagag
     3061 ctgagggaat ttgaactggc agacggaaac tggttactga acaatgcctg gtatgaaaga
     3121 aacatggcag ggtttaacga acagttgaaa gagaacctgt cattcacacc tgatgaactg
     3181 aaaacgctct tccggaaccg cctgaattta tcaccggaag cgaatgacga ttttctcgat
     3241 cgttgcctga acggcggtga ctggtatccc ttttcagaag gtcgccgttt tgtatcattc
     3301 catcacgtgg atgagcttcg tatctgtgcc tcctgcgggc tgacagaagt acatcatgcc
     3361 ccggaaaatc ataagccgga tccggaatgg tactgctcct ctctttgtcg cgaaacagaa
     3421 acactgtgtc aggaaattta tgaacgccct tacaacagct ttatttccga tgcaacggcg
     3481 aatgg
//
LOCUS       DAZGSP010000223         3470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZGSP010000223 DAZGSP010000000
VERSION     DAZGSP010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.225"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            144..1355
                     /gene="bluF"
                     /locus_tag="ACHK74_005126"
     CDS             144..1355
                     /gene="bluF"
                     /locus_tag="ACHK74_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFP0223772.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            1560..2291
                     /gene="bluR"
                     /locus_tag="ACHK74_005127"
     CDS             1560..2291
                     /gene="bluR"
                     /locus_tag="ACHK74_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFP0223773.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2512..2916
                     /gene="ycgX"
                     /locus_tag="ACHK74_005128"
     CDS             2512..2916
                     /gene="ycgX"
                     /locus_tag="ACHK74_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFP0223774.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2969..3079
                     /locus_tag="ACHK74_005129"
     CDS             2969..3079
                     /locus_tag="ACHK74_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223775.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHHANTIKLIFEM"
     gene            3315..3359
                     /locus_tag="ACHK74_005130"
     CDS             3315..3359
                     /locus_tag="ACHK74_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223776.1"
                     /translation="MFSKLAQSSIKAMF"
BASE COUNT         1043 a          708 c          707 g         1012 t
ORIGIN      
        1 agttttttcg ccaactagtg ttcaactttt tccatttttt atcaatgtaa agaaacggtt
       61 atgttttggt attaaaagaa agacacaaga agatatgtct gttaccgtct tactctcgcc
      121 tcacccatta ccctggattg ttaatgctta ccacccttat ttatcgtagc catatacgtg
      181 acgacgaacc tgtcaaaaaa atcgaagaaa tggtttcgat agcaaatcgc aggaacatgc
      241 agtctgacgt aacagggatc ttactgttta atggttctca ttttttccag cttctggaag
      301 gtccggaaga acaggttaaa atgatatatc gggctatatg ccaggatcca cggcactata
      361 atattgttga gctgctgtgc gattacgcgc ctgctcgccg ttttggcaaa gcgggaatgg
      421 aattatttga tttgcgcctg cacgagcgag atgacgtttt acaggccgta ttcgacaaag
      481 gcacatcaaa atttcagcta acttatgatg acagagcgct acaatttttt cgtacttttg
      541 tccttgcaac cgaacaatca acctatttcg agatccctgc cgaagactcc tggcttttta
      601 tcgctgacgg atctgataaa gaacttgatt cctgtgccct ttcaccaact ataaacgacc
      661 actttgcctt tcatcctatt gtcgatccct tatcgcggcg gataatcgct tttgaagcca
      721 ttgtgcaaaa aaatgaagat agcccatcag ccatagcggt tgggcagcgt aaagacgggg
      781 aaatctacac agcggatctc aaaagtaagg cgcttgcatt cacgatggca cacgcacttg
      841 agctcggtga taaaatgatt tcaatcaatc tattacctat gaccctggtt aacgaacctg
      901 acgcagtctc ttttttactt aatgaaataa aggccaatgc tctggtgcct gaacaaatca
      961 tcgttgaatt tactgaaagt gaagtcatat ctcggtttga tgagtttgcc gaagcgatta
     1021 aatcgctaaa ggctgctggt atcagtgtag caattgatca ttttggcgca ggttttgctg
     1081 gtttgttact cctgtcacgc ttccagcctg acagaattaa aatcagtcag gaattgatta
     1141 ccaatgttca taaaagtggg ccacggcagg caattattca ggcgatcata aaatgctgta
     1201 catcacttga aattcaagtc agtgctatgg gcgtggcaac accagaagag tggatgtggc
     1261 ttgaatccgc aggaattgag atgtttcagg gagatctgtt tgctaaagct aaattgaatg
     1321 gtatcccttc catagcgtgg ccagagaaaa aataattttc agcacattct ttcacatgat
     1381 ttcagtaaat catcgtgaaa atgaatacgt tacgtttaaa tttaatccac attaaaaata
     1441 atgtttcatc caatttagct gaaatcttat ttatactttg tacaatttta gcgtatattg
     1501 tacaaagtat tattgggtcg tgtacaggcg acggagattt gtgaccgcaa ggaggaattg
     1561 tggcttatta cagcattggt gatgttgctg aacgttgcgg gattaatcct gtcactctcc
     1621 gggcctggca acgccgctac ggtttgttaa aaccacagcg cagtgaaggc ggacaccgac
     1681 tctttgatga agaagacata caacgcatcg aagagatcaa gcgttggata agtaatggcg
     1741 tccctgtagg caaagttaaa gcattactgg aaaccaccag ccaggatacg gaagatgact
     1801 ggagccgcct gcaagaagag atgatgtcaa ttcttcgcat ggctaatcct gccaaactac
     1861 gcgcgagaat tatttcactg ggtcgagagt acccagtaga tcaattgatt aatcatgttt
     1921 accttcctgt tcgccagcgt ctcgtgcttg atcacaacac ctcccgcatt atgagcagta
     1981 tgtttgacgg cgcattaatt gaatacgcag caacctcgct tttcgaaatg cgccgtaagc
     2041 ccggtaaaga agccattctg atggcgtgga atgttgaaga gagagcacga ttgtggctgg
     2101 aagcatggcg tttatcattg tcaggatggc acatttctgt tcttgctgat cccattgaat
     2161 cgccgcgccc ggaactgttc ccgacgcaaa cattgattgt ctggacaggc atggcaccaa
     2221 cgagaaggca aaacgaactt ttacaacatt ggggtgagca aggttataaa gtcatctttc
     2281 atgcccccta atttcacctt tatcccgccc cttagacaga atgttataat ctgaacccac
     2341 aacagcgttt gtaccggagc tggtccgttg caaaatgctc accgggagaa ttcagatatt
     2401 ccctctatca cactctgtac atatcacagc caaaggacag accagattga agtggtaaaa
     2461 ccgatcgtgc acactacgca gtctcacaac taacaagagg ttattatcct gatggatcag
     2521 gtcgttgttt ttcaaaagat gtttgagcaa gtgagaaaag agcaaaactt ctcctggttt
     2581 tattcagaat taaaacatca ccgtattgca cattacattt attatctggc tacggataac
     2641 atcagaatta ttactcacga tgacacggtt ttgttattaa gaggaaccag gaacctgtta
     2701 aaagttagta cgaccaagaa ccctgctaaa ataaaagagg ccgcattgct tcatatttgc
     2761 ggaaaatcta catttcggga atactgttca acactagcag gcgcgggcgt tttccggtgg
     2821 gttactgatg ttaatcataa caaacgcagt tactacgcca ttgataatac gcttttatat
     2881 attgaagatg tagaaaataa taaaccatta atctagctaa agttggatgc ttaagaaatg
     2941 cttcataatt cagtaaggca ttagcataat gggaataaaa gtacagagac tatccctatg
     3001 gatgataaat actgtccttt tattgctacc cataaataat caccatgcca atactatcaa
     3061 attgatattt gaaatgtgat cacttgactt tctatacgtt attttataac ggttaatata
     3121 tttataaaaa caacgggtgt gccatacgcc cgtttcaata cttaacgcac atgtgttttg
     3181 gtttagtcat catccagtta tatgtatttt agccaggaac aggttaaacc tttcctatat
     3241 aactcaaaaa ttgaaacctt attctcatgt catgcttata ttcattatta tcgttatata
     3301 aaaaggcaac cataatgttt agcaaattgg cacaaagtag cataaaggct atgttttaat
     3361 tacaggatgt tcagtcattt gaatgtataa cattatagct aaacaaatct aaaacgaagt
     3421 gaataattta ttgttttcac taaatctcat tttgtttaac atccattgag
//
LOCUS       DAZGSP010000224         3385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZGSP010000224 DAZGSP010000000
VERSION     DAZGSP010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.226"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            59..352
                     /locus_tag="ACHK74_005131"
     CDS             59..352
                     /locus_tag="ACHK74_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFP0223777.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
     gene            complement(413..1533)
                     /locus_tag="ACHK74_005132"
                     /pseudo
     CDS             complement(413..1533)
                     /locus_tag="ACHK74_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(1542..2309)
                     /gene="sctT"
                     /locus_tag="ACHK74_005133"
     CDS             complement(1542..2309)
                     /gene="sctT"
                     /locus_tag="ACHK74_005133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFP0223778.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALFQHYHYELSTLNEIDIALFAAREIIIGLFIACLLSSPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTNGGLNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGFLARYASQLNAFAISLTV
                     KSALAFLILIIYFGPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(2311..2571)
                     /locus_tag="ACHK74_005134"
     CDS             complement(2311..2571)
                     /locus_tag="ACHK74_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP0223779.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQRVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(2581..3246)
                     /locus_tag="ACHK74_005135"
     CDS             complement(2581..3246)
                     /locus_tag="ACHK74_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP0223780.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTYFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYYNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVIISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(3236..>3385)
                     /locus_tag="ACHK74_005136"
     CDS             complement(3236..>3385)
                     /locus_tag="ACHK74_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFP0223781.1"
                     /translation="RIISLLPESEKNIEMRVNGALTGYGELVEVDDKLGVEIHSWLSG
                     HNNVK"
BASE COUNT         1156 a          666 c          547 g         1016 t
ORIGIN      
        1 ctctgtgaat gggataaaca ttattttaaa ctccaatctt gcgtaagaga aagttgatat
       61 gtcattagta ggatataata gtaacatcat aataaccatc gcgctgatat cgttgctctc
      121 tgcatgcacc agagaaaatt cattaaagga gaatcaaaat aaaagaagtg gcgaatccca
      181 agcgatagta gatgcaagac agttctttag cgaacacccg gaatatatat catcagcaga
      241 actggagcaa aagctatatc gcgaatttgc acaggttact acagttccag cttataaaga
      301 gatgagtatg tatcaattgc ttatcatttc acaagatcgt ttaactcatt aaataaggat
      361 ctacacgctt tttgcgtgta gattcttata aatttctgac tacgacagat gtttaatttt
      421 ttaaattatc gtctttattc tcgatttttg tgtcttcacc ctcaatgaat ttatcttctg
      481 aagagagctg ttcatcttga acaggttgcc cagcattctc cacatcttca agccacagca
      541 gaagacgtaa tatttcatct atattttcga agctaacata atcgtagcga cgatgggtag
      601 cataaatttt tcgtgctaat tttttatctg tgataattgg tattccgatt tcctttgcat
      661 atttccttac gcctaaggcc acctcattag ttactcttac agaaatcaat ggaataggtg
      721 aaagatgtgg cttaaagtat atccctattg caatgtgagt agggttggaa atcatcaaac
      781 gactattact gacatcagac ttcaattgct cagaaagaat ttcctgatgc gctctctgcg
      841 tttagactta atttctggat ttccttcttg ctccttgtat tctcttttaa cttcttgttt
      901 atccattttc atatctttca taaataaaaa atattcagcg ataaaatcaa aaattaagac
      961 aattatcata ctgccgagac aatacagtat gagaaggaat agcatctctc cccaatctga
     1021 taataaagat atgatatctc catcaagagt tttaaaaagc agtgatttat gattactcca
     1081 aaatactttg atcgccaatg caaaaaaaat aatataaaga attgctttaa caaattcttt
     1141 tacggttttt aagccaaata tacgctttaa gccatttact ggattcaatg aatcaaactt
     1201 aaatttaaca gcttcagttg ctaattgcat tttactttga aaccaggaga caagtattgt
     1261 tgtgaacatg caaaccagca aaaaaggaat gatcgttttc aaccaaccaa cccccatagc
     1321 tttaaaatag tcccaaatat ctggtttagc gttatgatca aggatgtatt caagaatcga
     1381 cataatgtga tgcacatcaa aaacatatcc gagatataaa gtacccacaa gcattattac
     1441 agagatggtt aagtctctac tttttagaat ttgccctttt ttagaagcat cctggagttt
     1501 cttttgtgtt ggcttttcag ttttattggc catgaaacac tctatttctc aatgtatagt
     1561 tgcaattgtt ctgggaaaaa ggataacggc attactcgtt cagccagtat tgggccaaaa
     1621 taaataatta atattaaaaa tgccagcgca cttttgacag tcagtgaaat ggcaaaagca
     1681 tttagttgtg aagcataacg tgccagaaat cccaggacag cctcaccgcc cagcataacg
     1741 gcaatcacgg ggcttgcgta aacgatagta tgagtcatta tgttattaat atatgagaat
     1801 aaaggctcta atttaggaaa attaaaattg cctgaaggcc ataaattata actttgccaa
     1861 agagtttcca gaataaagtt caatccaccg ttagtaagat aaaccgctgc agagaatagg
     1921 ttaaataatc ttgctaattc cgaagtatcg acaccagtag caggatccaa agtactactt
     1981 aacgttgcac cacgttggtt atcgataaaa ctacctatag caaggaaaat ccaaaacggg
     2041 gatgataata agcatgcaat aaacagccct atgataattt ctctggcagc aaacaaagcg
     2101 atatcgattt catttaaggt cgataattca tagtggtaat gctgaaataa tgcacatgat
     2161 accactatta ttattggatg acggactact gttggtatat tcccactggc gaaaaatggt
     2221 aacaaataga aagttggtgc caatcggcaa aaaccgagcg ctgtagcagc cagcagacta
     2281 tgcaactgat agagtatcgc ctcccccatc ctatcctctc gcgaatgcta aattcagcat
     2341 ttcgatacca aacgaatata atttttcacc ataccatccg ctcattaaaa aaaagcaaat
     2401 acttacgcat aataatttga cgccaaatgg caacgtctgt tcttgtaact gtgttaccct
     2461 ttgaaaaagc cctaccaata aaccaacaaa tgtcgcaact gcgataggtc ctgcagacat
     2521 aactaaaatc agatacagcg ccctattccc tgcaaaaaca atatcgtcca tattttttcc
     2581 tcagggatta atggataggt caaaatattg taaaatgagt ccttttgata acattgtcca
     2641 accgtccata gcgacaaata gaattaattt tatcggtgta gatatgatta ccgggctcat
     2701 catcatcata cctaacgtta acagaacgct ggatataacc agatccacta cgacaaaggg
     2761 taaatagata taaaaaccta taataaatgc actttttatc tcactcaatg catatgcggg
     2821 cagtaacgag aaaattgaaa tattatcatc atcgattatc tcttcattgt cttcagaact
     2881 attcaccttc tgaatctttt caaaaaagct aaccaactca ggttcagaat attttatcaa
     2941 atatgattta taaccactca tgcctgtctc aacaaaatta acaaccgacg ctacattatt
     3001 gaatgaaaga ttctcattct gactgttata atatatttcc ttcccaaccg gcatcattac
     3061 aaacattgac aatagcaaag caaccccatt aagtgtcata tttgatggca cttgctgaag
     3121 ccccagcgcg tttcggacaa taacaaaaac aatcgagaat ttaataaaat atgtcccgga
     3181 tgctattata aacggcaata gtgtaaataa tgatagtata gctatcagcg aaatactatt
     3241 tgacattatt atgccctgat aaccaagaat gaatctctac gcccaattta tcatccactt
     3301 caacaagttc gccatagcca gttagtgcgc catttacacg catttctata tttttctcag
     3361 attcaggaag cagagatatt attct
//
LOCUS       DAZGSP010000225         3349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZGSP010000225 DAZGSP010000000
VERSION     DAZGSP010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.227"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            5..1102
                     /locus_tag="ACHK74_005137"
     CDS             5..1102
                     /locus_tag="ACHK74_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFP0223782.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRT
                     DTQVNAGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKA
                     QNFEAVAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVD
                     YKINLLDKNDFTKALGVSTDDIVAVGLVYQF"
     gene            complement(1292..1675)
                     /gene="quuD"
                     /locus_tag="ACHK74_005138"
     CDS             complement(1292..1675)
                     /gene="quuD"
                     /locus_tag="ACHK74_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFP0223783.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(1761..1901)
                     /gene="ylcG"
                     /locus_tag="ACHK74_005139"
     CDS             complement(1761..1901)
                     /gene="ylcG"
                     /locus_tag="ACHK74_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFP0223784.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(1898..2260)
                     /gene="rusA"
                     /locus_tag="ACHK74_005140"
     CDS             complement(1898..2260)
                     /gene="rusA"
                     /locus_tag="ACHK74_005140"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFP0223785.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2257..2547)
                     /gene="ybcO"
                     /locus_tag="ACHK74_005141"
     CDS             complement(2257..2547)
                     /gene="ybcO"
                     /locus_tag="ACHK74_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFP0223786.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNSETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2540..2710)
                     /gene="ninE"
                     /locus_tag="ACHK74_005142"
     CDS             complement(2540..2710)
                     /gene="ninE"
                     /locus_tag="ACHK74_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP0223787.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPEPKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2710..3165)
                     /gene="ybcN"
                     /locus_tag="ACHK74_005143"
     CDS             complement(2710..3165)
                     /gene="ybcN"
                     /locus_tag="ACHK74_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFP0223788.1"
                     /translation="MNLPQDGIKLHRGNFTAIGRQIQPYLEEGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLINRGKTFATPAWVKDALKHTYLGYETKDLVDVVTGDITTI
                     QSLRHTSDLDIGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(3162..3263)
                     /locus_tag="ACHK74_005144"
     CDS             complement(3162..3263)
                     /locus_tag="ACHK74_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223789.1"
                     /translation="MRTHNPNSLLPPQMQKCTCDILYPAFDLCGGEA"
BASE COUNT          879 a          811 c          751 g          908 t
ORIGIN      
        1 cacgatgaaa aaattaacag tggcaatttc tgctgtagct gcatcagtac tgatggcgat
       61 gtctgctcag gcagctgaaa tttataataa agacagtaac aagctggatc tgtacggaaa
      121 agttaatgcc aagcactact tttcctctaa cgatgcagat gatggtgata ctacttatgt
      181 tcgtctgggc ttcaaaggcg aaacccaaat caacgatcag ctgactggtt tcggtcagtg
      241 ggaatatgaa ttcaaaggca accgcgctga atctcaaggt tcctccaaag acaaaacccg
      301 tcttgcattt gcaggcctga aattcggtga ctacggctca atcgattacg gccgtaacta
      361 cggtgtagca tacgacatcg gtgcgtggac tgacgttctg ccagaattcg gtggcgatac
      421 ctggacccaa acagatgtgt tcatgactgg tcgcaccact ggtgttgcaa cctatcgtaa
      481 caacgacttc tttggtctgg ttgatggtct gaactttgct gctcagtacc aaggcaaaaa
      541 cgatcgtagc gatttcgata actacaccga aggtaacggt gatggcttcg gtttctctgc
      601 tacctatgaa tacgaagggt tcggtatcgg tgcaacttat gcgaaatctg atcgtaccga
      661 cactcaagtt aatgcaggga aagttcttcc tgaagtattt gcttccggta aaaatgcaga
      721 agtttgggcc gcaggtctga aatatgacgc taacaacatt tacttggcca ctacctattc
      781 tgaaacccag aatatgactg tatttgctga tcacttcgtt gctaataaag ctcaaaactt
      841 cgaagctgtt gcacaatatc agttcgattt cggtctgcgt ccgtccgttg cttacctgca
      901 atctaaaggt aaggatcttg gagtatgggg cgatcaggac ttagtcaaat atgttgatgt
      961 aggtgcaacc tattacttca acaaaaatat gtctactttc gttgattaca aaatcaacct
     1021 gcttgacaaa aatgacttca ctaaagcact cggtgtaagc actgatgaca tcgttgctgt
     1081 aggtctggtt taccagttct aatctgatta cgaaaaagat atgttgcggg aggctttgcc
     1141 tccccaacat ataagtggct ccctcaagcc acttccttta ggagcacaac cttgcttcta
     1201 actatataaa ccttctgtta tatattaccc tttatttttg ggggcgttgc aacgccccat
     1261 ttttaataat ttttagtaaa caattggcat attaattaga gttattaaca acgatatcca
     1321 tctctaaccg gatatctaat gccattaaca tcccttcaat tatgccctca gccttctgta
     1381 accttttccc gatataacca tcagagcagc aatgcttacc tgccagtgac atgaatgtca
     1441 taccgactac ataataatct actaataaat cgtgcaaatc gctgttgttc tttttcagac
     1501 gggccatgca cccgcaaatg atcatcgcgt catcgtcaca acattgcggg cgagatttta
     1561 cttttgaagg aattaatccc ttaaaaccgg cggcaatgga cgaccaggtc acatcttcat
     1621 gattattagc cgcccacgct ccccaacgct caagaaccat ctgaatatca cgcatcaact
     1681 tactccacaa aaatcagacc agaacgccaa ttacaagcaa aaatcaacaa aacagtatta
     1741 gttgattgtt atctctgact tcatactcct gctcctgtca gggttttggc gtaattcttc
     1801 agtattcggt aatcggtcaa aacagaaccg gggaaacgat ataagcgcag acgcccccag
     1861 cggtggcgaa gaagttctgc catattaaac tcaaacatca ttcattcccc atttcggtga
     1921 tggtcagttc cagcctccca cctttggtaa caggcatctt cacaacgcgg taatcaacga
     1981 cctgagcatc atccagccag aaacctgctt tagtgagtgc gtcaaaagcg gctttttgca
     2041 gattatccag gtcacggcga cggcgatccg gcatgtggca ctcaatgcgg attttcacag
     2101 gcatagccag gccgatatcc agcattgcgt ttttaatgat tcgggcgacg ttatcgcggt
     2161 atgcctgccc ctctgcgctg acgtgcgtgc gcccgcgatt atggcggtaa tagcgattat
     2221 tgctcggagg ccagggtaat gtgatactgt aggtattcac gccttaataa ccccctcttt
     2281 cagccagata acctgtgttc tcgccatacc ttccagcgcg cattcttttg catatgcagc
     2341 atcgacaaaa tgtgtgcggc ggtcgatttc gtcgtggcag gcagaacatg caatggtggc
     2401 aatcaggtct ggcggtttga taccggtacc gcacaatcca gccagccgga tatgtgccag
     2461 tacagacgtt tcagaattgc cattacatac gccagggatt cttacctggc attcccgacc
     2521 acgcgctgct tttctcaaat cagccatgat tcctccttgc tgccagtcgc aaccattttt
     2581 tatcaaccag gctagcggta tatccgagca gtgttggtat ttcggatggc ttcggctcag
     2641 gcttacgctt acgacgattt gatactctgt agatgtgtcc gttcatgaca cgaataagcg
     2701 gtgtagccat tacgcctcct gcttgtcacg gagcagctgg aactcgcagc tctgcggaat
     2761 agtcaggtgg cagccaatat tcaccgccca ggcttcaacc ttacacagga agacatacat
     2821 ctctccgata tcaagatcgg aggtatggcg taacgactgg atagtggtga tatcaccggt
     2881 tacgacatca accaggtctt tggtttcata accgagatat gtgtgtttga gagcatcttt
     2941 tacccaagct ggagtggcga acgttttacc cctgttgatg aggtattcac tgatttcgct
     3001 gtaccacatg tggctgagtg cattctggga aagactgcgt ttctcacgcc acggtttaag
     3061 caccatgcga aagcatttgc cctcctccag ataaggctgg atctgccgac cgatagcggt
     3121 gaagttaccg cgatgtaatt tgatgccatc ttgtgggaga ttcacgcttc acctccgcag
     3181 aggtcaaacg ctggatacaa tatatcgcag gtgcatttct gcatctgtgg agggagaaga
     3241 gagtttggat tgtgtgtgcg cataaacgtc cccgtttagc gcagaagtca ccggagttgt
     3301 tcaggctccg atgacatgat tatggcgagt tgattatcgc aaatcaaag
//
LOCUS       DAZGSP010000226         3348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZGSP010000226 DAZGSP010000000
VERSION     DAZGSP010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.228"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..912
                     /locus_tag="ACHK74_005145"
     CDS             <1..912
                     /locus_tag="ACHK74_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFP0223790.1"
                     /translation="AWLKEKGAFDQPYWMMKASLPAGFNKVITDVGPGKLNLGGCVIE
                     VMGTYEAAIVRVTIGEYGATGFINGTVCTCTVYGDTQYFHWRVDYSTKNKPDTVSQRD
                     ASTTQKGVVQLSSDTDSNDETKAATPKAVKAAMDVANEAKTKAEEAAAGGGVPGPKGE
                     KGDTGPAGPAGPQGPKGDTGAAGPAGAQGPKGDKGDPGEAGPAGLAGPKGERGEPGPM
                     GPPGKDGATSLVSGLRIGGKKEVAHHEVITFPSLDGAQPTIPGMNIVPDGAVILNIRS
                     QAYKITAVTYGFLQVHNGSQWVTIKTE"
     gene            976..1647
                     /locus_tag="ACHK74_005146"
     CDS             976..1647
                     /locus_tag="ACHK74_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFP0223791.1"
                     /translation="MIIKNLKHYTPEKPDVPGAMYLKSEDGQDWYECQSLFSAETLKL
                     VYDSNGIITSISKDVSMLWPVNQSVAEVADTEENRKADISGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVPVTAEELIALSEAAEQAMFTKGMEIHIRQRTMKKELEKLTSADEILAYRV
                     GWAQE"
     gene            2022..3173
                     /locus_tag="ACHK74_005147"
     CDS             2022..3173
                     /locus_tag="ACHK74_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP0223792.1"
                     /translation="MDKSLSRSVISVVIGGILFGVSAGVLAQDASENKLIASGSIPEN
                     TDLSASARADGEIALAVGTGASADKEIATSVGSWSAARGQQSVALGGGTYAYADASTA
                     LGSIAFVDNTATYGTAAGNRAKVDKDATEGTALGAKATVTNKNSVALGANSRTTRDNE
                     VYIGYEAEPGKAYKTRVLGGLSDGTRPSDAATVRQVDRVKDSVEQLAQDTNTRLVVEA
                     KKSREYTDSRTTVGVNSDGTLTRAEGASKTLAVNDGLVALSGRTDRIDATVGSVDRRV
                     TKNTQAIQSTSRQLQEHNTRLNSHQRQINENHEEMKRAAAQSAALAGLFQPYSVGKFN
                     ATAAVGGYSDKQAVAVGVGYRFNEQTAAKAGVAASDGDVSWNAGVNFEF"
BASE COUNT          925 a          672 c          994 g          757 t
ORIGIN      
        1 gcctggctga aagaaaaagg ggcatttgac cagccttact ggatgatgaa ggcatcactg
       61 cctgcgggat tcaataaagt catcacggat gtcgggccgg gaaaactcaa tctgggtggc
      121 tgtgtcattg aggtgatggg aacgtatgag gctgccatag tccgggtcac cattggcgaa
      181 tacggtgcaa cggggtttat taatggcacg gtctgtacct gtacggttta cggcgacaca
      241 cagtatttcc actggcgggt ggattacagc acaaaaaaca aaccggatac ggtcagccag
      301 cgggatgcca gcacgacgca gaaaggtgtg gtgcagttaa gcagtgatac tgacagtaat
      361 gacgaaacaa aggcagccac gccgaaggcc gtgaaggcgg caatggatgt ggcaaatgaa
      421 gcgaaaacaa aggcagaaga ggctgcagca ggaggtggtg ttcccggtcc gaaaggagag
      481 aaaggggaca cggggccagc aggtccagca ggaccgcagg gacctaaagg ggatacggga
      541 gctgctggcc cggcaggcgc acaggggcca aaaggtgaca aaggcgatcc gggagaagca
      601 ggtccagcag gtctggcagg tcccaaaggt gagcgcggtg aaccgggacc gatggggcct
      661 ccggggaaag atggcgcaac ttcgctggtt agtgggctgc gtatcggagg gaagaaagaa
      721 gtcgcacacc atgaggtgat tactttcccg tcactggatg gagcacaacc aacgattccc
      781 ggcatgaata tagtccctga tggtgctgta attctgaata tccggtcaca ggcttacaaa
      841 attactgctg taacttatgg ctttcttcag gttcacaatg gaagccagtg ggtaacgatt
      901 aaaacggaat aacgtgactc acactggttg ttgcgttcca ttatgcccaa ccgggcatta
      961 aatacggaga ataatatgat tattaaaaac ctgaagcact atacccctga aaaacctgat
     1021 gtgccgggtg cgatgtacct gaaatcagag gatggtcagg actggtatga gtgtcagtcg
     1081 ttattttcag cggagacgct gaagctggtt tatgacagta acggtataat caccagcata
     1141 agtaaggatg tctctatgct gtggccggta aaccagagtg tggcagaggt tgcagatacg
     1201 gaagaaaacc gcaaagctga catctcgggg cgctggggtt ttgacgggga gaaaatcacg
     1261 gacctgctga ccgcggagaa agcgcgcggg atgaagggcg atgaaattaa cgcctggcgt
     1321 aatgcgatgg aagcggcgaa ctacacgttt gagcacaatg ggcgtaaatg ggactacggg
     1381 aagtcaacgc agacgcgtct tgagccgtcg gtggctgcag cgaaagcggg gaaactgccg
     1441 gaggcgtttt tctggacgga tgcggaaaac aatgatgtgc cggtgacagc agaagagctt
     1501 attgcgctga gtgaagcggc agagcaggcg atgttcacta aagggatgga aatccacatt
     1561 cgccagcgca ccatgaagaa ggagctggaa aagctgacca gtgcggatga gatcctggca
     1621 tacagggttg gctgggcaca ggagtaattg gtggaaaaga gggattgtcc ctttctgttt
     1681 ttactgaaag agtcgtaata attacgcagg gaaatatcat ggctgaacta aacagttaaa
     1741 ttttgatgat attgtgatgt tgctcagaaa ttacagtgta agcagatgtg aaatattcag
     1801 cggcatgaga tcagtttttt atttagtatt gctctgcctt gtgctccagt atttggagta
     1861 aaggtgtaaa aaatacacta agatatttcc taaaaattaa aattaaattt gtggggtttt
     1921 ttaggaatat cctcatgggt gccgaaatac atcagatttt ctttattcat ccgatgtatt
     1981 tcggtttgtt tgtatacaaa taaattcatg aggaatcata catggataag tctttatcac
     2041 gaagtgttat ttccgttgtt attggcggta ttttatttgg tgtaagcgct ggcgttctgg
     2101 ctcaggatgc atcggagaat aagcttatcg cctcagggag tatcccagaa aatacagatc
     2161 tttctgcttc agctcgcgcg gatggagaga ttgcgctagc tgttggtaca ggggccagcg
     2221 cggataaaga aattgcaaca tcagtgggat catggtctgc agcacgagga cagcaaagtg
     2281 tggctttagg tgggggaaca tatgcttatg cagatgcatc tacagccctg ggaagcattg
     2341 catttgttga taacacggct acatatggca cggcagcagg taaccgagcc aaagttgata
     2401 aagatgcaac agaaggtact gcgttagggg caaaggcaac agtaacaaat aaaaacagtg
     2461 ttgcattagg agctaattca cgcacaaccc gtgacaatga ggtttatatc ggttatgaag
     2521 cagagccggg taaggcttat aaaacccggg tgcttggtgg tctgagtgac ggtactcgtc
     2581 cctcagatgc tgcaaccgtt cgtcaggttg accgtgtaaa agacagtgtt gagcaactgg
     2641 ctcaggatac gaatacccgc cttgtggttg aagcgaaaaa gtctcgtgaa tatacagact
     2701 ccagaacgac agttggtgtt aactccgacg gaaccctgac ccgtgcagaa ggagccagca
     2761 aaaccctggc tgttaatgat gggcttgtag ctttgtccgg cagaacagac cgtattgatg
     2821 ctactgttgg tagtgttgac cgccgggtta ctaaaaatac acaggctatt cagtccactt
     2881 cccgtcagtt gcaggagcac aacacacgtc tgaacagtca tcagcgtcag attaatgaaa
     2941 accacgaaga gatgaaacgt gcggcagccc agagcgcagc gctggcaggt cttttccagc
     3001 cgtacagtgt gggtaaattc aacgccactg ctgccgttgg tggttacagt gacaaacagg
     3061 ctgttgccgt gggtgttggt taccgtttca atgagcagac agcagcgaaa gcaggtgtgg
     3121 cagcgagcga tggcgatgta tcctggaacg ctggtgtgaa ctttgagttt taattgctga
     3181 cagtgacatg aagcatgacg gcaggcgtgg agttttgtct gccgtttttt ctcatgtgag
     3241 catgaaacag gtggcagcga tgaaagtgat tctggcgacc cgcaaccgtt atctggagta
     3301 tggcctgcag cagatgcttg aggggtacag gataatactg gcgaggga
//
LOCUS       DAZGSP010000227         3206 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZGSP010000227 DAZGSP010000000
VERSION     DAZGSP010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.229"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            152..241
                     /locus_tag="ACHK74_005148"
     CDS             152..241
                     /locus_tag="ACHK74_005148"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFP0223793.1"
                     /translation="MTAYRNVFIQDDPGMHFRRVIRNAEGQRR"
     gene            419..802
                     /locus_tag="ACHK74_005149"
                     /pseudo
     CDS             419..802
                     /locus_tag="ACHK74_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
     gene            1312..3090
                     /locus_tag="ACHK74_005150"
     CDS             1312..3090
                     /locus_tag="ACHK74_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HFP0223794.1"
                     /translation="MRNARTILGIIRKRGAEGKSLDQIYRMLFNPELYIAAYAKIGSN
                     KGAMTKGVTDETVDGMSHKKIHDIISLLRNERYKFSPARRIYIKKKNGKMRPLGLPTW
                     SDKLLQEVMRMILEAYYEPQFSQFSHGFRPQRGCHTALTVIAKTWTASTWFIEGDIKG
                     CFDNIDHEILLHILREQIKDNRFIRLIEKLLYSGYMEEWKCHKTWSGTPQGGIISPLL
                     SNIYLNKLDQYVEHVLLPKYNKGKSRARNREYEVQNGIEHYNRKRGRLDISRAARKKK
                     QTLPSKDPFDPHYRRLRYVRYADDFLLGFSGPKSEAEEIKKEITLFLQNNLSLEVSQE
                     KTLITHGRTDKARFLGYDIQVGQCDTFRNRKKRRSVNGDIRLLVPADVVREHSKRYFL
                     KGRVRRRPELLEESDYTIVHNFQTRFRGLANYYQLACDRSKKLSRLKGIMEHSLMLTL
                     ANKHKTWVSRIYQKYGTRCMMTNGKSYKCLQIRIDRVNKAPLTATWGGISLARNKNYR
                     NIVLVDEKDLLFYGRVERLKAILASECAICGSTQNIQVHHIRALKDLAKRGRTPTASQ
                     RRMAARRRKTLVVCHQCHVAIHQGKL"
BASE COUNT          935 a          719 c          814 g          738 t
ORIGIN      
        1 gttaactgat ccctgaagtc agtttcatgc ttcagcctct gccacaacgt atggcagagg
       61 ctatttatca ggagaaacca gatatgccgg gttacacaca gagtatgtcc tggctgaagg
      121 atctttctca tacgggcagg cggttgctgt cataaccgct taccgcaatg tgtttatcca
      181 ggatgacccg ggaatgcatt tccgtcgggt tatccgcaac gcggaaggtc agcgacgctg
      241 acgctgccgg aactctgaag ctgatgccgg aaaacagctt aatgcgtggc tcgccagtgg
      301 agggcttctc agacaataaa cgtcattgat ttcgtccatc aggccgcgtc ttctccggga
      361 gacgcggctt tttcatttat tgcaccagta aatcttaacc accgataagg agcaaagtat
      421 gcgattagtc agtcgttttg gttatgctgc aaaccagata cgccgtgacc gtccgctgac
      481 acatgaagag ctgatgcacc atgtgccggg gattttcggg gaagagaaac acacatcccg
      541 aagccagaac tatacgtaca tccccaccat caccgtactg gaaagtctgc agcgggaagg
      601 cttccagccc tttttcgcct gccagacccg tgtgcgcgac ccgggccgcc ggggatacac
      661 aaaacacatg ctgcgtctgc ggcgggccgg agagataaac ggagaacatg tccctgaaat
      721 tattctgctc aactcccatg acggtacctc cagctaccag atgctgccgg gttacttccg
      781 gttcgtctgc cagaacgggt gcgccgtgtc ggcgtggtaa gggattcctg attcaatcag
      841 gagtggtcgg aatgaaatac cactgagtca acgccttacc tgaggctgaa aggtcaaggg
      901 gacaatagca tggcggtaaa ccggagaggg ccagaaaacg tgtatggctg aactgttccg
      961 ggagactcgc gcaatatggt gaaagctaca ctgcctgaac cctaataacc aagcgggata
     1021 aggcactccc cgtcactacc acgtttaaga gtgatgataa ccggatcaga cgaaatgccg
     1081 ttggtcgtcc caatcattcc ccggttaagc cgtacccagt gagggtacca aggttatgag
     1141 tagggcacct aagttgcgct gttacgttcc ttaccgtgta aacacgggat tgtctaaggg
     1201 gcgcgagccc tagggcaacg gagccaccgt agtagtccgg gtccgggaaa gccggataca
     1261 tggcgaaggg tggcaggtta caacgatgtt taaaattgag aggtacgcga gatgcgaaac
     1321 gccagaacaa tattaggcat catccgcaag agaggggctg aaggtaaatc gctggatcag
     1381 atttatcgga tgctttttaa cccggagtta tatattgctg cctacgcaaa aatcggtagc
     1441 aacaaagggg caatgacaaa aggagttacc gacgaaaccg ttgatggaat gtcgcacaaa
     1501 aagatccatg acattatcag tcttctgcgt aatgagagat ataagttctc cccagccaga
     1561 aggatctata ttaagaaaaa gaacgggaaa atgcgtcctc ttggcctgcc aacatggagt
     1621 gataagctgc tccaggaggt catgcgtatg atccttgagg cctattacga gcctcagttc
     1681 agccagttct ctcacggttt tcgcccacag cgtggttgtc atacagcact gacagtcatc
     1741 gccaaaacat ggacggcttc cacatggttt attgaagggg atattaaagg gtgtttcgat
     1801 aatattgatc acgaaatatt gctgcatata cttcgggaac aaattaaaga taatcgattc
     1861 atccggttaa ttgagaaact gctttactcc ggatatatgg aggaatggaa atgccataaa
     1921 acctggagtg gaacacctca gggtggaata ataagcccgt tgctctctaa tatatacctg
     1981 aataaacttg accagtacgt ggaacacgtg ttattgccta aatataacaa gggtaaatcc
     2041 agagcaagga atcgagagta tgaagttcag aatggtattg aacattacaa tcgtaaaagg
     2101 gggagactgg atatatccag agcggcacgt aagaagaaac aaacgttgcc gtcaaaagac
     2161 ccttttgacc cgcattatcg gcggttgcga tatgtgagat atgcagacga cttcctgctc
     2221 ggattctctg gcccaaaatc agaagctgag gaaatcaaaa aggaaataac cctgtttctt
     2281 cagaataatc tgagtcttga agtttctcag gagaaaacgc ttattaccca tggaagaaca
     2341 gataaagccc gttttcttgg atacgatatt caggttggtc aatgtgatac gttccgtaac
     2401 agaaagaaaa gacgcagtgt caatggcgac atccggctac ttgttcccgc cgatgtagtg
     2461 agagaacaca gcaaacgtta ctttcttaaa ggacgagtaa ggagacgacc ggaattactg
     2521 gaggagagtg attacactat cgttcataat ttccagaccc gttttcgtgg tttagctaac
     2581 tattatcaac tagcctgtga cagaagtaaa aaattatctc gtctgaaagg catcatggaa
     2641 cattccctaa tgttaactct ggctaacaaa cataaaacgt gggttagccg gatctatcaa
     2701 aaatatggaa cacgatgcat gatgactaat ggtaaatctt acaaatgtct acagataaga
     2761 atagatagag ttaataaagc accattgact gcaacatggg gcggaatatc tctggcccgt
     2821 aacaaaaatt accgaaatat tgtgttagtt gatgagaaag acttgctctt ttatgggcgc
     2881 gttgagcgac tgaaagccat actggcttct gaatgtgcaa tatgtggctc aacccaaaat
     2941 atacaggttc accacattcg ggcactgaaa gatcttgcga aacggggaag gacgcccaca
     3001 gcatcacaaa gaaggatggc agcaaggcga cgtaagacac tggttgtatg tcaccaatgc
     3061 catgtcgcga ttcatcaggg aaaactctaa ttaccttgcg gatttgttgt aactggagag
     3121 ccggatgcga tgaaagtcgc acgtccggtt cggagagggg ctgacggaaa agtgccaata
     3181 cagcaactcg tcggcagcct acccta
//
LOCUS       DAZGSP010000228         3185 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZGSP010000228 DAZGSP010000000
VERSION     DAZGSP010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.230"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(91..618)
                     /gene="mobB"
                     /locus_tag="ACHK74_005151"
     CDS             complement(91..618)
                     /gene="mobB"
                     /locus_tag="ACHK74_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFP0223795.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(600..1184)
                     /gene="mobA"
                     /locus_tag="ACHK74_005152"
     CDS             complement(600..1184)
                     /gene="mobA"
                     /locus_tag="ACHK74_005152"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFP0223796.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     AQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1254..1523
                     /locus_tag="ACHK74_005153"
     CDS             1254..1523
                     /locus_tag="ACHK74_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFP0223797.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1600..2586
                     /gene="srkA"
                     /locus_tag="ACHK74_005154"
     CDS             1600..2586
                     /gene="srkA"
                     /locus_tag="ACHK74_005154"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFP0223798.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2603..>3185
                     /gene="dsbA"
                     /locus_tag="ACHK74_005155"
     CDS             2603..>3185
                     /gene="dsbA"
                     /locus_tag="ACHK74_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFP0223799.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFV"
BASE COUNT          797 a          771 c          780 g          837 t
ORIGIN      
        1 gaaggccatc cgtcaggatg gcctttttgc attggcgcag aaaaaaatgc cggatgcgac
       61 gctggcgcgt cttatccagc ctactcttgc ttatccgttc tgcttttgca tccactccac
      121 cacaaaatca gccagcccct caacatcatt aatatccagt aatgcgacat caagattaag
      181 cggcacatca ctggctacag caataacatg cctgtctatc actaattctt caggtcgatg
      241 tccggctcca tcgcgaaaca gcacaatctt tgcgatctct tcatgcttaa acccttcgac
      301 cagaatcaaa tccagctttg aggtatccat tcgacttgcg agaaactgta gatccagctc
      361 ttcttcgtct ggtgtttccg tcatcaaggc ccatcgttgc tggctggcaa cgatggtttg
      421 tgccgcgcca gccttgcgca gctcatagct atctttgcct ggcttatcaa catccatatc
      481 atggtgcgta tgcttaatca gccctggacg gatccctctg gcgcataatg ccgggatcag
      541 ttgtttcaaa agcgtagttt ttccggtacc actccacgcg gcaaaggcga gtaacggtat
      601 catcgttttt cctgccatcg ggcaagctcc tctggcgtat tcacgttaat aaatgcatct
      661 ttattatcgc tgaaatcaac cgcatgaccg ccagccagac gcataaatgc cattacccgg
      721 cgttctcctg cttgcagata ttccagtaat aaaggctcaa ttgcgcggtt taccagagca
      781 atagtcgggt gatcgcgttc accgtcgtgg acccacacga caggcgcatc tttgcgctga
      841 tgattaagcc gggctgctaa atcctcggga atataaggag tatcgcacgg acaaaacaaa
      901 aaccactcgc ctgcttcctg ctgcattact gaaagcattc ctgccagagg gcctgggtaa
      961 tccgccagtg aatcttcaat cactttcaga ccgcttgcct gatagatttc ctgatgacga
     1021 ttagcattaa ccacgacgtg agagagctgc gccataagcg cgtcagcgac atgttgccat
     1081 aatggtttgc cgtttaattc cagcaatcct ttatctacgc cgcccattcg tctggcttta
     1141 ccgcctgcca gcacaacgcc tgttatcgtc gtcatcagat tcaccgatat cgcctctttt
     1201 attgtgggat tgaccctgct aacgtgtctg tctcaagagt aaggagcatt actatgaaat
     1261 gtaaacgtct gaatgaagtt attgaactcc tccagccagc ctggcaaaaa gagccagacc
     1321 ttaacctgtt gcaatttttg cagaaactgg cgaaagagtc aggttttgac ggcgaactgg
     1381 cggatttgac ggatgacatt ctgatctatc acctgaaaat gcgtgattcc gcgaaagatg
     1441 cggtgatccc gggtttgcag aaagattatg aagaagactt caaaacggcg ctgttacgcg
     1501 ctcgcggcgt aattaaagag taaaagcttg taagcggtgc caccaaaatc atcgtgaaat
     1561 gatatccttc atcattcgta atgttttccg gatgatggga tgaacaacag cgcttttact
     1621 ttccagacac tacacccgga taccatcatg gatgctctat ttgagcaagg gatccgggtg
     1681 gattccggtc ttaccccgct taacagctat gaaaaccgtg tctatcaatt tcaggacgaa
     1741 gatcgtcgac gttttgtcgt caaattttat cgccctgaac gttggacagc cgatcaaatc
     1801 ctcgaagaac atcaatttgc gttgcagttg gtaaatgatg aagttccggt cgcagcacct
     1861 gtggccttta acggtcagac tttattgaat catcagggat tttatttcgc tgtttttcca
     1921 agcgtcggtg gtcgccagtt cgaagctgat aatatcgatc agatggaagc ggttgggcgt
     1981 tatttagggc gtatgcacca gacggggcgc aaacagcttt ttatccatcg cccgaccatc
     2041 ggtctgaatg aatatctcat tgagccacgc aagctgtttg aggacgctac actgatacct
     2101 tccgggttga aagcggcatt cctgaaagcg acagatgagc tgatcgccgc cgttacagca
     2161 cactggcggg aagatttcac cgttctgcgg ctacatggag actgccacgc cgggaatatt
     2221 ctctggcgcg atggtccaat gtttgttgat ctggatgatg cacgtaatgg tccagctatt
     2281 caggatttgt ggatgttgct caatggcgat aaagccgagc agcggatgca actggaaact
     2341 attattgaag cttatgaaga atttagcgag ttcgacaccg ctgaaatcgg actgattgaa
     2401 cctttacgcg ccatgcgttt ggtttattat cttgcctggc taatgcggcg ttgggctgat
     2461 cccgcgttcc cgaaaaattt tccgtggtta accggggaag attactggct gcgacagacg
     2521 gcgactttta tagaacaggc aaaagttcta caagaacccc ctttgcaatt aacacctatg
     2581 tattaatcgg agagagtaga tcatgaaaaa gatttggctg gcgctggctg gtttagtttt
     2641 agcgtttagc gcatcggcgg cgcagtatga agatggtaaa cagtacacta ccctggaaaa
     2701 accggtagct ggcgcgccgc aagtgctgga gtttttctct ttcttctgcc cgcactgcta
     2761 tcagtttgaa gaagttctgc atatttctga taatgtgaag aaaaaactgc cggaaggcgt
     2821 gaagatgact aaataccacg tcaacttcat gggtggtgac ctgggcaaag atctgactca
     2881 ggcatgggct gtggcgatgg cgctgggcgt ggaagacaaa gtgactgttc cgctgtttga
     2941 aggcgtacag aaaacccaga ccattcgttc agcatctgat atccgcgatg tatttatcaa
     3001 cgcaggtatt aaaggtgaag agtacgacgc ggcgtggaac agcttcgtgg tgaaatctct
     3061 ggtcgctcag caggaaaaag ctgcagctga cgtgcaattg cgtggtgttc cggcgatgtt
     3121 tgttaacggt aaatatcagc tgaatccgca gggcatggat accagcaata tggatgtttt
     3181 tgttc
//
LOCUS       DAZGSP010000229         3154 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZGSP010000229 DAZGSP010000000
VERSION     DAZGSP010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.231"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..748
                     /locus_tag="ACHK74_005156"
     CDS             <1..748
                     /locus_tag="ACHK74_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0223800.1"
                     /translation="GGGFSDNYTHVQVGADKKHELDGLDLFTGVTMTYTDSNAGSGTF
                     SGKTKSVGAGLYASALFDSGAYIDLIGKYVHHDNEYTASFAGLGTKDYSSHSWYAGAE
                     VGYRYHVTEDAWIEPQAELVYGAVSGKQFSWKDQGMSLSMKDKDYNPLIGRTGVDVGK
                     SFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGGKDGRMLMSVGLNAEVRD
                     NIRFGLEFEKSAFGKYNVDNAVNASFRYSF"
     gene            complement(1171..1329)
                     /locus_tag="ACHK74_005157"
                     /pseudo
     CDS             complement(1171..1329)
                     /locus_tag="ACHK74_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
     gene            1478..1603
                     /locus_tag="ACHK74_005158"
                     /pseudo
     CDS             1478..1603
                     /locus_tag="ACHK74_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(1663..2169)
                     /gene="yciE"
                     /locus_tag="ACHK74_005159"
     CDS             complement(1663..2169)
                     /gene="yciE"
                     /locus_tag="ACHK74_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP0223801.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(2215..2715)
                     /gene="yciF"
                     /locus_tag="ACHK74_005160"
     CDS             complement(2215..2715)
                     /gene="yciF"
                     /locus_tag="ACHK74_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP0223802.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(2801..2980)
                     /gene="yciG"
                     /locus_tag="ACHK74_005161"
     CDS             complement(2801..2980)
                     /gene="yciG"
                     /locus_tag="ACHK74_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP0223803.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
BASE COUNT          836 a          665 c          718 g          935 t
ORIGIN      
        1 cggcggtggc ttcagtgaca actacacaca cgttcaggtg ggtgcggata aaaaacacga
       61 actggacggt ctggacctct tcaccggggt gaccatgaca tataccgaca gcaacgccgg
      121 cagcggcacc ttcagtggta aaacgaagtc agtgggtgcg ggtctgtatg cttcagccct
      181 gtttgattcc ggggcctata tcgacctgat tggtaagtat gttcaccatg ataatgagta
      241 tacggcgtcc tttgccggac tcggcacaaa agactacagc tctcattcct ggtatgccgg
      301 tgctgaagtg ggttaccgct atcatgtcac agaggatgcc tggattgagc cacaggcgga
      361 actggtctat ggtgctgtat ccggtaaaca gttcagctgg aaggaccagg gaatgagcct
      421 gtctatgaag gacaaggact acaatccgct gattggccgt actggtgtgg atgtgggtaa
      481 atcgttctcc ggtaaggact ggaaagtgac ggcacgtgct ggtcttggct atcagtttga
      541 cctgctggcg aacggagaaa cggtactgcg tgatgcatcc ggagagaagc gtattaaggg
      601 cgggaaagac ggtcgcatgc tgatgtctgt gggtctgaac gcagaggttc gtgacaacat
      661 ccgttttggt ctggagtttg agaaatcagc ctttggtaag tacaacgttg ataatgctgt
      721 caacgccagc ttccgttatt cattctgacg gttgttactg aaggggatta tggcctgacg
      781 gctccctgta aataaagaca ggcacagaag attgtgagtt acttagtgtt ccaccaaaaa
      841 gtaaggtcgc aactctgtgc ctgcttcgta agtgtacagt gagggccata tggtcataac
      901 tgaagcatcc gacgatgtgg tgtctaccca aaaagcgacc tgtgaatatt aggctggtta
      961 agtgcgtatt ttcatggaag gagatcaccc aatagccccg atctccttta tgttaatacg
     1021 cattaagcgg gtaaaacagg aagattaaga aagggagagt ttttcgactc cccaaccaag
     1081 agaagcagct tataactacc agatagttgt tatgtcatac gtgacggata atgtcataaa
     1141 aaatatgtca ttgccgtgat tataatcgcg ttattggata aagcagtctt cagcggtttc
     1201 ccaggcttcc tgcagcgttt cctgtacaaa ttcgtgcgcg gattctttat ttacaactct
     1261 taaaatggtg agtccatcat tgctggtggt tctgacaatc acgtccacat ggtcatatcc
     1321 gttatttatg gaatgactat aaggggggat aagcctctgc aaaacaaaaa aagactttaa
     1381 caaaatcaat cagttaaaaa cacagaaaaa cggtatgaaa aatcaccata aaagtttgtt
     1441 tttcctttta taaatagctg cttatcacat tttttataat ctaccagtgt ggtacatgga
     1501 tatcgatacc accgccaatt ataagctagg cggtgcacag caacacgata gcgtacgcct
     1561 cgatccgtgg gtgtttatgt tctcagcagg atatcgtttt taattccgca caaaaacgac
     1621 cccgtaatat acggggtcaa taaggacatg gtataaaggg tattatttct tcgcttctac
     1681 gccatcagtt tcagaacgaa ttaaaaattt ctcagttgtt tgcggaatat gctgaatcag
     1741 ccagtcggcc atttgctttt cctcatttaa aatcgcttcg atgatcggaa ttgagactgt
     1801 atcaccggca ttttttgctg ctgctaatag tgaggtgtaa caggcgattt caaattgctc
     1861 gaagacatat ccgctaatag agcctttaac tatttcatca gaagggaata taccaccgat
     1921 tgactgccca agcgcagcca ttttactcat ggaatcttta atgactgaac gtgaaatgtc
     1981 attacgatca agaatagttt ccagttgaac aatctggttt ttggtttcac taagatgttg
     2041 ttcaatacga gcgcgtagtt caggataatt atctatacga ctggccatgg attcaagcat
     2101 agattcggct tgcttttcca ttgcgtgggc gtcacgtaac cagtcatgat aatgttcaat
     2161 acgattcatg aaaatgtcct ctttataaaa tgactaaaag ttaaaattca tatttcaggc
     2221 tttattttcg gctttcttat ttacgttatt aagggccaga tcagtcagtt tgatgtcggt
     2281 ggccttttct tcttccaggg tttctttcag aagcttcgct gctttacggt aacctaattg
     2341 ttcagccagc gtcgctaatg tcccgtaact ggcaatctca taatgctcga ctttctgtgc
     2401 tgcggcaatc agtgcggcat cacgcacttc gtttttctcg gtactttcga tgacctcatt
     2461 agcttcttca ataagacctt ccattgccac acatttcatg cgcttaattt tcaggttcga
     2521 ttcggactcc acaacttgat caatacgttc aatctgtcca tgagtttcct cgaggtgcgc
     2581 atgaaaagcc tgacttaatt tttcatttga tgttgctctt gcgagttttg ccagtgcccg
     2641 ggttaattgt ttttctgcgc tgtaggtatc tgaaagcagg tgaataaata catcttcaat
     2701 ggtcttcata ttcatatttt tctccagtga aatcactgcg agccatacgg cccgcagatg
     2761 caattatctt tatcagctga tattaataat taacggtgaa tcaggattta cctgatttat
     2821 taccaccgct ttgttgaccg ccttttttac ccgcttcaga tgcgcgttgc ggatcatttt
     2881 taaaattacc gccgctatgc tgaccgcctt tacggcctgc gtcggatgcc ttctcacggt
     2941 cttcggcgaa atttcctgaa ccaccacgat gttcggccat gttatttctc ccgttgcgtt
     3001 gcattgtttc attaatatga gtgttgtgtg tcgacactca ttaaaattag tcgctaatga
     3061 gaattagtca aattaagcgc aacgagaaga tagagggaaa atatattttg aggaacattc
     3121 tggatatatt aacaattacc tgaggaataa gtga
//
LOCUS       DAZGSP010000230         3123 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZGSP010000230 DAZGSP010000000
VERSION     DAZGSP010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.232"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..659)
                     /locus_tag="ACHK74_005162"
     CDS             complement(<1..659)
                     /locus_tag="ACHK74_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFP0223804.1"
                     /translation="MEITKNKNATIKKQSSVGCFSMFTLSFSITFIPVFLTNRQELIS
                     RGLLVPALFLAEFLILVPLYFIFFRKREGLGRGSFRLDVFFTLFSLIMIIQLFVPYLL
                     AVRKTEEWMISQMSFSGAILWINIFSSVLLVPVYEEIVFRGCLFNSFKFWFNDNIYIS
                     AIVTSVIFSALHLQYTDFRTFLMLFLISLVLISAKVKSNGLLMPILLHMAMNAVITGI
                     Q"
     gene            complement(759..1217)
                     /locus_tag="ACHK74_005163"
     CDS             complement(759..1217)
                     /locus_tag="ACHK74_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223805.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIIFLIILLVCILSLITTIK"
     gene            1675..2184
                     /locus_tag="ACHK74_005164"
     CDS             1675..2184
                     /locus_tag="ACHK74_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223806.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
     gene            2273..2896
                     /locus_tag="ACHK74_005165"
     CDS             2273..2896
                     /locus_tag="ACHK74_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0223807.1"
                     /translation="MRTRIDYLADKYSFTELNELPRLHQQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWDISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVIGNENTSSPYQQIAREVKAPRERLKHALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKSGMPVVTTTAEVHDNLTGTTRKVPAYHL"
     gene            2985..>3123
                     /locus_tag="ACHK74_005166"
     CDS             2985..>3123
                     /locus_tag="ACHK74_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223808.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGQADNRNRVAEIVTRKL
                     QD"
BASE COUNT          921 a          601 c          709 g          892 t
ORIGIN      
        1 tattgtattc ctgttatgac tgcattcatc gccatgtgaa gtaatatggg cattaacagg
       61 ccattcgact ttacttttgc actgataaga actaacgata tcagaaagag catcaaaaat
      121 gtacgaaagt cagtatattg tagatgtaat gcagaaaata tgactgatgt tacaatagca
      181 gagatgtaaa tattgtcatt aaaccagaat ttaaaagagt taaaaagaca ccctcgaaat
      241 acaatctctt catatacagg aacaagaagt acggaagaaa atatattaat ccataaaatg
      301 gccccggaaa aagacatttg cgagatcatc cattcttccg ttttcctcac tgccagcaga
      361 taagggacaa ataactgaat aatcattatt aaagagaaaa gagtaaaaaa aacatccagt
      421 cgaaaagaac ctcttcctaa tccttctctt tttcggaaga aaataaaata cagtggaaca
      481 agaattagaa attcagcaag aaataatgct ggaacaagaa gtcccctgga tataagttcc
      541 tgcctgtttg taaggaatac aggaataaag gtaatagaaa atgataaggt aaacattgaa
      601 aagcagccga ctgatgactg tttttttata gttgcatttt tattttttgt tatttccatt
      661 tatattccct tacttgtcgt ttaaaattct tctatataac aggaagaggt aaccatagaa
      721 tgaacgttta ttttcctgta tcagaacagc cataactttt attttatagt tgttatcagt
      781 gaaagaatgc aaactaatag aataatgaga aaaataatgg agaaaaagcg acggaccgga
      841 cgccatagtt catattctcg tgtatcagcc tcacgtatca acctttctga aatggtttcg
      901 gtttcttttc ctcgcaccag aatatgtctg taatatttcc tcatgacata taccgaaaac
      961 tgaaaccaga ttaatatgga tggacacaca acacataaac ttctgatgat atcacccggg
     1021 aaagtatctt gggcgggtaa aagaaagtca tatactctgg ataaaatcat catcgagata
     1081 aatgagacgc cagcacctat ccagtattcg atatgagaag atgctgcatt atatgcgtca
     1141 gtaaaatttt tctttcttaa tgaaggtgga agtgctgaaa gttcaggaat gcgttttctt
     1201 gtccagtata ttttcatata gtaactcata taaaaagtgt tctactttac tatgatgatg
     1261 acgatttata aaaggcttat aatgactact tctcctgtca ttctcgaatt tcacgataat
     1321 gatagagata atcattatca attgatagtg tcagtaattt tgacttacat cacactttca
     1381 tggtttaatc aggtgattga tttaatgaaa agagtgtgtt tgtggatgta ttgtgtatat
     1441 gtcttaccgg gcatctgttc ttacattaat taatatattg ctatttaagt attggtagta
     1501 ttgtcagtaa cattcttttg gttaatttag ttattattcc tcactgatta attgtccttc
     1561 cgttcttatt gtttgtaact tgtgtttgta tcgtaccttt caggtgttga tgcgcgcttt
     1621 actgtgtctg caccggcttg acaaaattca acaggggttg aaaaggaaca tttcgtgcaa
     1681 ataaccgaag ccttaatttc agagccgggg gaaatccggc gttttgttca gcaggcggtg
     1741 gaccactggc caaatctgtt ggcatttcac ttcacgctct attcggcaga aggaatctat
     1801 ggtcaacaga ttcagacatt ttgtagttcc tttcaccgac gggtacacga acgtattacg
     1861 gagcataatc acacggtcag cccatcagcg ccggtggtat tacgctggtt gcgggaacaa
     1921 catgaaggag cacagatacg atgtctgttg ctgctcagcc agacgagtat ttgccatccg
     1981 cgagtcggcg tcatggctga tgaagaatgt gcgcaactgg tggatttact gcaacagacc
     2041 tggtcggtga taagtgctgg cggacaatgc cgggtggaaa gatgttttcg ggttgcccgg
     2101 cctggttcat cagggcaata cgtggcgtta aaaacggctg ttcagtcctt tatgtcgcag
     2161 gttattgcaa caatcatccg gtgattgttg ggaggaatat caatgcttcc gttaattttt
     2221 actggctgat aaagttttgt ctgctgaatc tttatacgga attcctttca gtatgcgtac
     2281 acgaattgac tatcttgcgg ataaatacag ttttactgaa ctgaatgaac ttccacgcct
     2341 tcaccagcag tggcaggatg ttctggagga gtgccggcag acagaggccg ggccggaaga
     2401 acggctgcgt attgcactgc tgaatgtgga ttacgtcacc agttttgaac tgccttttcg
     2461 cttgttgctt acccggacac cacaactgat tgccgcgctg cgtgaagaat gggatatcag
     2521 ccagaaaaat gtggtgttca acgataaacg ctttggctgc gtgtacagcc tgaaggccag
     2581 cctttccggt gtgccggata cataccggta tcatctgtct catcgtattc gccgggtgat
     2641 tgggaatgaa aacacatcat cgccatacca gcagattgcc cgggaagtga aagcgccccg
     2701 tgaacggctg aagcatgcgc tggaggcggg gttgctggtg actgcactgg acggcctgtt
     2761 ctggttcggc agtcagcgta ttgcggctga tgtcctgagg ttgagaaaga gcggaatgcc
     2821 ggtggtgaca acgaccgcgg aggtgcatga taatctgacg ggaacaaccc gcaaagtacc
     2881 ggcctaccat ctctgattcg accatcaggg ggaatattgc tctggcagca gcagtacgac
     2941 attctttatc cccgagcctg aaaacatgtg ctaaggaaac gggaatggca aggtccgatt
     3001 acgatattat caatctgtct ccggaacatg aactgaatga gtggctggca gagagaggtt
     3061 atgccggaca ggcggataac cgtaaccggg tggcagaaat tgttacccgc aaattgcagg
     3121 aca
//
LOCUS       DAZGSP010000231         3047 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZGSP010000231 DAZGSP010000000
VERSION     DAZGSP010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.233"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            94..726
                     /gene="comR"
                     /locus_tag="ACHK74_005167"
     CDS             94..726
                     /gene="comR"
                     /locus_tag="ACHK74_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFP0223809.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(788..1327)
                     /locus_tag="ACHK74_005168"
     CDS             complement(788..1327)
                     /locus_tag="ACHK74_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP0223810.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1554..2858)
                     /gene="ndh"
                     /locus_tag="ACHK74_005169"
     CDS             complement(1554..2858)
                     /gene="ndh"
                     /locus_tag="ACHK74_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFP0223811.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          750 a          807 c          738 g          752 t
ORIGIN      
        1 aaagcaatta tttttatggg atctggactg gtgaacggag gtgattattt ttataataac
       61 ctttcattca cagtcaccag gtacaacggc atcatggcaa ctgactcaac acaatgtgta
      121 aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg ccgcgcttga taaggccatg
      181 aaattgttct ggcaacacgg ttatgaagcg acttctcttg cggacctcgt cgaagcgacc
      241 ggagccaaag cgcccacgct atacgcggaa tttaccaaca aagaggggtt atttcgtgcc
      301 gttctcgacc gctatatcga tcgttttgcc gctaagcatg aagcacagct gttttgtgaa
      361 gagaaaagcg tggagtctgc gctggctgac tattttgctg ccatcgccaa ctgctttacc
      421 agcaaagaca ccccggcagg ctgcttcatg atcaacaact gcaccaccct ctccccagat
      481 tcaggagata tcgccaatac gttgaaatca cgccatgcga tgcaagagcg cactttgcag
      541 cagtttttat gtcaacgaca agcgcgcggg gaaatcccga cccactgtga cgtgacacat
      601 ctggcagaat tccttaattg tattattcag gggatgtcga tcagcgcacg cgaaggtgca
      661 tcgctggaaa aactaatgca gattgccgga acgactttgc gtttatggcc cgaactggtg
      721 aaataagtcg caaaaaaagc ccctcagcct gagcgaatga ggggccaaat tgcagagtac
      781 agccttgtta tactttgtta ttcaaaatca gttgcccatt actatctagc gggatctgcg
      841 tacccggatc gcggtccatg cggattttgc cctgctgatc gccaatctta taggtcacat
      901 cataaccgag cattttttct gacttgtcat acaccgtttt acaacgctgt tgcgtagtcg
      961 tgtaagtatc gctttcctgg agagagccct ggatctggtt accggcatat ccaccaccca
     1021 gcgcccccac aacagtggcg acatctttac cacgaccacc accaaactga tgccctatca
     1081 cgccgccagc aacagcgccg agcaccgacc cggtaatgcg attttcatcc tgcaccggtc
     1141 gacgatgggt cactgtgacg ttgcgacact cctgacgcgg tgttttaacc gtttccttga
     1201 ttggggttgc agaaacaacc tgagcgtatt gcgggccccg ttcaaacacg ttcagactgg
     1261 ccactgccgc tacgcccagc gcagctgcga caccaatccc gatacccgcc aacattgatt
     1321 tattcacggg aacacctcct tctttgcagt tatgccaatc atgcaagcga aggagaaaag
     1381 cagctatcag acagaagatg aaaagcgcgg gaatggctgg aaatacttaa ggattcggag
     1441 gagtctgaag gaatgcctga tgcgacgctt aacgcgtctt atcaggccta ccgctcgtac
     1501 catttgtcca gggcggatga ggcgtttatg ccacatccgc cagtgtacgt cgattaatgc
     1561 aacttcaaac gcggacggat aacgcggtta atactcccca ccagcatcat taatccggtt
     1621 ttaaagtaac catgcagcgc aatctgatgc attcggtata gcgagatata tacaaagcgc
     1681 gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt tacccatcag gctaccgacg
     1741 gtggagaagt tcgacagcga taccagcgaa ccatgatctt tatactgata atttttcagc
     1801 ggcttaccgt tcatctgcgc cagtatgttg ttcattgcgc aagtcgccat ctggtgtgca
     1861 gcctgagcac gcggcggaac aaagccccct tccggacgcg ggcatgacgc gcagtcgcca
     1921 atagcgtaaa tgtctggatc gcgggtggtt tgcagcgtcg gttccaccac cagctggttg
     1981 atacggttag tttcaagacc accgatatct ttcaggaagt ctggcgcttt gatcccggct
     2041 gcccacacca tcagatcagc ctcaatatat tcgccatctt tagtgtgcag gccgccttca
     2101 tcagcactgg tgaccatggt ttgcgtcagc acgcgaacgc caagtttcgt tagctcgttg
     2161 tgggccgcag cagagatacg tggcggtaat gcaggcaaaa tacgttctcc cgcttctacc
     2221 agcgttacgt tcagggcttc gttggtcagg cctttgtaac cgtagctgtg cagttgcttg
     2281 accgcgttgt gcaattcagc ggagagttct acacccgtcg cgccgccgcc gacaatcgca
     2341 atgttcactt tgccatttgc gcccaggttg gcggagtatt tcaggaagag attcagcatc
     2401 tcctggtgaa agcgacgcgc ctggtgcggg ttatcgagga aaatgcagtt ctctttgaca
     2461 cctggcgtat tgaaatcgtt agaggtgcta cccagcgcca ttaccagggt gtcataggcg
     2521 attttacgtt ccggaaccag cagttcacct ttctcatcgc gcagttctgc aatagtgatt
     2581 gttttcgctt cacgatcaat atcaatgacg gaacccagct ggaactggaa accatgattg
     2641 cgcgcatggg ccagatagct caacgcatcg acgccttcat caagcgagcc agtcgccact
     2701 tcgtgcagca gcggtttcca caggtggctg tggttacgat cgaccagcgt aattttggct
     2761 tttttcttac gtcccagctt atgccccagc tgtgttgcca tttccagccc accagcaccg
     2821 ccgccgacaa tcacaatctt tttcaatggc gtagtcaacg tgaccccctt aaatttatta
     2881 accaattgtt aattaatggt tattaacata gctcttaatt aacaacaggt tacagcaatg
     2941 aaaatgttct tcgctattga gaataacatg aatggtgcat tggtcatacc aaaattgatg
     3001 ttaatcaagt tttgttgctg aaaagatagg cgactttacc gggtgag
//
LOCUS       DAZGSP010000232         3029 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZGSP010000232 DAZGSP010000000
VERSION     DAZGSP010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.234"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..701)
                     /locus_tag="ACHK74_005170"
     CDS             complement(<1..701)
                     /locus_tag="ACHK74_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223812.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFVLLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRSIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAIRQTQEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLT"
     gene            complement(822..>3029)
                     /gene="agn43"
                     /locus_tag="ACHK74_005171"
                     /gene_synonym="flu"
     CDS             complement(822..>3029)
                     /gene="agn43"
                     /locus_tag="ACHK74_005171"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFP0223813.1"
                     /translation="DVTQNTGGALVTSTAATVTGTNRLGAFSVVAGKADNVVLENGGR
                     LDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLADSGAAVSGTRSDGKAFS
                     IGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAGTTTLNNGATLTLSG
                     KTVNNDTLTIREGDALLQGGALTGNGRVEKSGSGTLTVSNTTLTQKTVNLNEGTLTLN
                     DSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSGATWNIPDNATVQSVVDDLSH
                     AGQIHFTSARTGKFVPATLQVKNLNGQNGTISLRVRPDIAQNNADRLVIDGGRATGKT
                     ILNLVNAGNSGTGLATTGKGIQVVEAINGATTEEGAFVQGNRLQAGAFNYTLNRDSDE
                     SWYLRSEERYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLG
                     HDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDG
                     SRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKASSGNNDFRARGRGWLGS
                     LETGLPFSITDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHN
                     DMTFGEGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSSRGDMRVGTSTAGSGMTF
                     SPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSINGSSAEGYSSQATLNVTF"
BASE COUNT          626 a          885 c          842 g          676 t
ORIGIN      
        1 gtcagccaga tgcgctccgt cagcgtttca ggaaggctgt aatcgttttg caaactgctc
       61 agccacgatt tactgttgag caccggttgt tcttcctgtg tctggcggat ggcactggtc
      121 agtcttttca gcgcctggat ctggctggcc gcccatagca gcgtcactaa tgcagccagc
      181 cagtacaaca acccgcccca ccacatcagt gagggtgaaa tatcgaactg caatgcgatc
      241 aatggtgcgt atgcagaaca cgccataagg aaaaaagcag tataaaaaca gcagtaatac
      301 ccgttaattt ccttgctgat atcttcaggt gtgagcccgt tctgctgaat gcttcttatc
      361 tgcacgggaa gagccagcag accagcggcg agagcggtgg tcagaataaa ttttacagag
      421 tcaaaagtat gagcctccag tgcgatatgt ttaccatcaa gtccccacat gaagctgatg
      481 atcattccgg caatcgtcag aaatacgcct gccagccagc gaatatggaa taaccgggtt
      541 atgcgttcag tgatatagcc agccagtaaa gccgggccga acagacacag caatacgaat
      601 cctgccagcg cagacgccgt gctggttaat gctctgaaca aaaggcgaag aatggcaaat
      661 cctgcgatca gcaggatcag cgcgccgact atctgtaaca tagtcactcc gtgatgaata
      721 tcatgttatt gtgaatgcca gtgaatgtgg cactgaaacg ttttcccccg tccgggtccc
      781 ggtcatgatg accgggacca cagagaggca atgcagaatt atcagaaggt cacattcagc
      841 gtggcctgac tgctataacc ttcagcgctg ctgccgttga tgctgtgggc ataaccggcc
      901 tgaacgccca gggtgatatt ttcccggaca cgggcttcca gtccggcctg cagatccagt
      961 gaagtgccat tctgtgaggg agagaacgtc atcccgctgc ctgcggtgga ggtccccaca
     1021 cgcatatcac cccgggagct gaaggtgcgg ataacggaag gctgcaccca ccagttcacc
     1081 ggcagttcac gcacactgtg ttttgcactg tcacgcaggg gggcacggga tgaggtgcct
     1141 tcaccaaagg tcatatcgtt gtggctgccc agacggaaac cggcacgcac atgctgtgca
     1201 ctgccatgcc cgaacttcac ataaccggcg ttgtccttac cgtcatccag cgaaagcccc
     1261 tgccaggtgt actgcagccg tggctccagc atcaggttgt cagtgatact gaagggcaga
     1321 ccggtttcca gtgagcccag ccagccccgg ccccgtgcgc ggaagtcgtt attgcccgat
     1381 gacgctttca tactgtggcg ggttccctgt gccacaatgt cagcccacag gccggaggag
     1441 gtgtgtgtca ggttcatata tccgcccagg ctgccggcat catcccggac cgtgccggcg
     1501 cgggagccgt catcatcctt aacatcaacg gaagaatggc ctgcagcacc atacactccg
     1561 gtcgtcagtg acataccggc gacctctgtt ctgagcaggt caccctccag acggacgaag
     1621 ccatagctgc cgctgctttc cggcgtggcc ccacgggcaa taccgccgtt gttatcgtga
     1681 ccgagatggc cgccctgaat gctgagacgg acgctgttat tttcaccgtt tacaccggtc
     1741 tgatggctgc gggagcctgc cagaatccgg tcatagtcca ttgcctgtgt cagcatggag
     1801 gcgtacaggg ggacttcagc acgataacgt tcttcactgc gcagatacca gctctcatca
     1861 ctgtcccggt tgagggtgta gttaaaggcc ccggcctgca gcctgttccc ctggacaaaa
     1921 gccccttcct ccgtggtggc accgttaatg gcctcaacca cctgaatacc cttaccggtg
     1981 gtcgccagcc ccgtaccact gttgccggcg ttcaccagat tcaggatggt ttttccggtt
     2041 gccctgccac cgtcaatgac cagtctgtca gcattgttct gcgctatatc cgggcgtaca
     2101 cgcaggctga tggtgccatt ctgcccgttc aggtttttca cctgcagggt tgccggtacg
     2161 aacttccctg tgcgggcgga ggtgaaatga atctgtcctg catggctgag gtcatccacc
     2221 accgactgaa ccgtggcgtt atcggggata ttccaggtgg caccggatgt cagggtgaca
     2281 ttcgtggggt caatggcacc gttcagcact gtgctgccgg tcagcttcag ggccgtgccg
     2341 cgctgagcga tgacatccgt ggtgacggta ctgtcgttca gcgtcagcgt gccttcattc
     2401 aggttgacgg ttttctgggt gagtgtggtg ttgctgacag tgagtgtgcc acttccggat
     2461 ttttccaccc tgccgttacc ggtaagagcg cctccctgca ggagtgcatc accttcacgg
     2521 atggtcaggg tatcgttatt caccgttttc ccggaaaggg taagcgtggc accgttattc
     2581 agcgtggtgg tgcccgccag tgtgccgccc ctggcggtga acagtccgcc atttaccgtg
     2641 gtatccgtgg ccgtatcacc ggcgttcagt gtgaaggaac tgcctttttc cagcatcagg
     2701 gcatccgcct gaccgcctcc gatactgaat gcctttccgt cgctccgtgt accactgaca
     2761 gcggcaccgg aatcggccag cagtacaccg ccattcccca tggatacggt ggtggcggca
     2821 ccaccgttgc ggacatccag cgttccgcca tcatccacac gggtattagt ggctgtgtgt
     2881 ccgctcagca catccagacg tccgccattt tccagtacga cattatcagc cttacccgcc
     2941 acaacggaga atgctcccag gcggtttgtg ccggtgacgg ttgcagctgt actggtaacc
     3001 agtgcaccgc ccgtgttctg ggtgacatc
//
LOCUS       DAZGSP010000233         3000 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZGSP010000233 DAZGSP010000000
VERSION     DAZGSP010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.235"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..183)
                     /locus_tag="ACHK74_005172"
     CDS             complement(<1..183)
                     /locus_tag="ACHK74_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0223814.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSD"
     gene            complement(183..>3000)
                     /locus_tag="ACHK74_005173"
     CDS             complement(183..>3000)
                     /locus_tag="ACHK74_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFP0223815.1"
                     /translation="YSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGA
                     MTEDDARPEALRRFELMVEEVARNASAVAQNTAAAKKSASDAGTSAREAATHATDAAG
                     SARAASTSAGQAATSAQEASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSE
                     TNAAASQKSAATSASTATTKASEAATSARDAAASKEAAKSSETNASTSASSAASSATA
                     AGNSVKAAKTSETNARSSETAAEQSASAAADSETAAASSAREASIKAEEAAVSATAAG
                     KSAESAASSASTATTKAGEATEQASAAARSASAAKTSETNAKASETSAESSKTAAASS
                     ASSAASSASSASASKDEATRQASAARGSATTASTKATEAAGSAAAAAQSKSTAESAAT
                     RAETAAKRAEDIASAVALEDASTTKKGIVQLSSATNSTSESQAATPKAVKAAYDLANG
                     KYTAQDATTAQKGIVQLSSATNSTSESQAATPKAVKAAYDLANGKYTAQDATTAQKGI
                     VQLSSATNSTSETLAATPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLN
                     STTMSFSGGAGWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGN
                     PKGITGALWQRTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHT
                     SPAYSANKPEGLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAY
                     PKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTS
                     SFDYGTKSTNNTGAHTHSLSGSTNAAGNHSHRDGRRFNPSVFKDTYQYGYTSSGQNTW
                     GVQGSVGMSTGWLANTSTDGNHSHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTI
                     TVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT          575 a          823 c          793 g          809 t
ORIGIN      
        1 atcactgttg aaaacagcca caaagccagc aggaatatct ggtggtgcaa tatcggtact
       61 gtttgctggc agacctgtat gaggcggaat atatgcgtca ccttcaccaa taaattcatt
      121 agttccggcc agcagattat aaatttttat ggtccgtggt tgttcactca ttctgaatgc
      181 cattatgcaa gcctcacaat atagttaaat gcgatgtttt tgacggtgtt ttccgcgtta
      241 ccagcagcgt taacggtgat ggtgtgtcca tgtgaaccaa tcgcaacgga gtgcgtatga
      301 gcaccaatac cgacagtatg cgcgtgtgca cctgcagatg ctgctgtgcc ggacagtgaa
      361 tggctatgat taccatctgt actggtattc gctaaccacc ccgtagacat acctactgag
      421 ccttgtacac cccaggtatt ttgacctgag cttgtataac catattgata agtatcttta
      481 aaaacactgg ggttaaatcg acggccatct ctatggctgt gattaccagc tgcattcgtg
      541 ctgccactta aactatgggt atgcgcacca gtgttattcg tggatttagt gccgtaatca
      601 aacgacgatg tggttttcgt ccccaaatcc gtactggatg cgctggcgct gtgggtatgc
      661 gatttaatgc cgtcctgttc ctgagacaat acggcccgac cactggcagg tttgccctta
      721 atcgtccagc cacgcatatc agggatcacg cctgacggat aagcggctgc aagtttcggg
      781 tatgcagatt tgtcaaaagt ctgcccctgc atcagggcat agccagacgg aacggtatct
      841 gatggccacg ggattggtgc gccaggcgga taaaactgct ctgatggcgt atagagtgaa
      901 taaactgtac cgtccgttaa cccttccggc ttattagcag aatatgctgg tgacgtatga
      961 atcgtcacgc tggcattact ggtataatcc cattgaatat ttacaccagt cgcataattt
     1021 ccgattgcaa cgtaaatatc gtaagtatca ccagatgtat tgacccaggc aaaatttgta
     1081 aaccctgtcg atgtgcgctg ccataaagca ccagtaatcc ccttcggatt accattacct
     1141 gcacgtaaaa caagttcaga tatacctgcc tgttgaggtg accccacgtt aaatccagcg
     1201 ccaccaatca acgtaattga aacaacagaa ctcgcctgtg gcatggttac cgttgctaat
     1261 ttgaaccaac cagcaccacc gctgaatgac attgttgttg agttaagcgt accaatatct
     1321 ttcggcgtca gtgttatatc cgctgaaagc gccttaccat tcaccttacg ggcagaaggt
     1381 acccgaccat tcgcattgtc attagctgct ttcactgctt tcggtgtcgc agcaagcgtt
     1441 tcagatgtgc tgttggttgc actactgagc tggacaattc ctttttgtgc tgtcgtagcg
     1501 tcctgtgcgg tgtatttccc gttagccagg tcatacgcgg ccttaacggc ttttggcgtt
     1561 gccgcctgtg actcggaagt gctgttagtc gcactgctga gctgaactat ccctttctgt
     1621 gctgtcgttg catcctgtgc ggtgtatttc ccgttagcca ggtcatacgc ggccttaacg
     1681 gcttttggcg ttgccgcctg tgactcggaa gtgctgttgg tcgcactgct gagctgtact
     1741 atcccctttt tcgtcgtgct cgcatcctca agcgccacgg cggatgcaat atcctctgcc
     1801 cgttttgctg ctgtctcggc gcgcgttgcc gcggattccg ccgtactttt gctctgtgct
     1861 gccgctgccg cactgccagc agcctctgtc gccttcgtgg atgccgtcgt ggcgctgccc
     1921 ctcgctgctg acgcctgtct ggtcgcctca tcttttgaag cagacgcaga tgatgccgat
     1981 gacgccgccg aactggcgga cgatgcggca gccgtttttg aggattctgc gctggtttcc
     2041 gacgctttcg cgttcgtttc ggatgtcttc gctgcggaag cagacctcgc tgctgcgctg
     2101 gcctgttcag tggcttcgcc agccttcgtt gtggctgttg aagcggatga tgcggcgctt
     2161 tctgccgatt ttccggcggc ggtggcactg actgctgcct cctctgcctt aatggacgcc
     2221 tcacgggccg atgatgcagc tgctgtctca gagtctgccg cagctgaggc gctctgttcc
     2281 gctgccgttt cagaagacct ggcattcgtc tcggacgttt ttgccgcctt cacggaattt
     2341 cctgccgccg ttgccgagga ggctgcactg ctggcgcttg tggaggcgtt cgtttctgat
     2401 gattttgccg cctctttgga ggccgccgca tctctggctg aagtggcggc ttctgacgct
     2461 ttcgtcgtcg cagtggatgc agaagtggct gctgattttt gtgacgctgc agcattcgtt
     2521 tctgacgttt tcgccgcacc ggcactggtg gccgccgcgc tttttgagga ctctgcagcg
     2581 gcagcacttt ttgatgcttc agtggccttt gttgatgccg ttcctgcgct ggaagacgcc
     2641 tcctgagccg aagtcgcggc ctgtccggct gacgtgctgg ctgcacgcgc tgagcctgca
     2701 gcatcagtcg catgggttgc cgcctcacgg gcagatgtgc cggcatcact ggctgacttc
     2761 ttcgcggctg ccgtgttctg tgccaccgcg gacgcgttac gcgccacctc ttccaccatc
     2821 agttcaaaac ggcgcagagc ctccggacgg gcatcatcct ccgtcatggc accgagaaaa
     2881 tcattcagcg taccgggtcg ggaatcttca tacacggtga tggtcccggc atgtgacggc
     2941 gggaatcctt ccaccaacag aataacgctg tactgaccgt actcaacgtc catgctgtaa
//
LOCUS       DAZGSP010000234         2985 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZGSP010000234 DAZGSP010000000
VERSION     DAZGSP010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.236"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            27..248
                     /locus_tag="ACHK74_005174"
     CDS             27..248
                     /locus_tag="ACHK74_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223816.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDACFKD"
     gene            229..696
                     /locus_tag="ACHK74_005175"
     CDS             229..696
                     /locus_tag="ACHK74_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFP0223817.1"
                     /translation="MRASKIEKETRMYGTCETLCRALAAKYPGDTPLMLVIWSPEEIQ
                     ALADGMDIALTDHEIRTVLARLEDIPEDQRTESGISSGVAMEIINNVRENRQVTVPAE
                     LLASLIQTAEQALWKREWAARDHGLAVPECVTRRQAVVNQVRILLKNNTHEND"
     gene            741..1511
                     /locus_tag="ACHK74_005176"
     CDS             741..1511
                     /locus_tag="ACHK74_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223818.1"
                     /translation="MNETLNALIYRHASNLLLAQGWPEETDVDQRNPKYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVHDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVSECVEHILASGLVCDVLR
                     IPDEPSRRWFDRDILREVVLDARDEIRSMADALAKIRK"
     gene            1927..2352
                     /locus_tag="ACHK74_005177"
     CDS             1927..2352
                     /locus_tag="ACHK74_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0223819.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            2399..2818
                     /locus_tag="ACHK74_005178"
     CDS             2399..2818
                     /locus_tag="ACHK74_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFP0223820.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIARDWNLSD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRVMRQALDGENG"
BASE COUNT          633 a          784 c          926 g          642 t
ORIGIN      
        1 actggtttat gccggaggct gcgtaaatga actatgccgg acacgaaaaa ctgcgcgccg
       61 aagtggcgga ggtggccaat gccatgtgcg acctgcgtac aaccatgaat gagatggagc
      121 agcggtacag ctttaatgcc gacaccctgc cggaacgtct ggtgcgtcag acgctgtttc
      181 gcgcaaaccg cctcctgatg gaggcatata ccgaaattct ggaactggat gcgtgcttca
      241 aagattgaga aggagacgag aatgtacgga acatgcgaaa cgctctgccg ggcgctggca
      301 gcaaagtatc cgggagatac gccgctgatg ctggttatct ggtccccgga agaaattcag
      361 gccctcgctg acggaatgga tattgccctg accgatcatg aaatcaggac tgtcctggcg
      421 cgcctggagg acatcccgga agaccagcgg actgaatccg gtatttcttc cggcgtggcg
      481 atggagatca tcaataacgt gagagaaaac cgccaggtga ccgttccggc tgaactgctg
      541 gcgtccctga ttcagaccgc tgaacaggca ttgtggaaac gtgaatgggc cgcccgggat
      601 catggcctcg ccgtcccgga atgcgtcacc cgccgtcagg cggtggttaa tcaggtccgc
      661 atcctgctga aaaacaacac acacgaaaac gactgatgtt atcgccgcct ccgggcggcg
      721 atacagggag ttcatgaatt atgaatgaaa cgctgaatgc actgatttat cgccacgcca
      781 gcaacctgtt gctggcgcag ggctggccgg aagaaacgga tgttgaccag cgtaacccga
      841 agtatccggg ctggatcagc atttacgtgc ggctggatgc gccccggctg gcgacgttac
      901 ttatcaaccg tcacggcggc gtactgccgc cgctcctggc ctctgccatt cagaaactga
      961 ccggaaccgg ggcggaactt gttctgtccg gcagtcagtg gcagtcgctg ccggtacttc
     1021 cggcagacgg aacgcaggtg tctttcccgt atgccggaga atggctgacc gaagacgaaa
     1081 tcagggccgt tcttgatgcg gtgcacgatg cggtacgcag tgtcagttgc cgggtggcag
     1141 aagatgcgcg gcgtatccgg gcggcgctta ccacaaccgg tcagacgttg ctgacccgcc
     1201 agacgcgccg ctttcgcctg gttgtgaagg aaagcgatca cccctgctgg ctcgatgaag
     1261 acgacgaaaa cctgcccgtg gtgctcgatg ccattctgaa ccggggagca cgtttttcct
     1321 ctgtggaaat gtacctggtc agtgagtgtg tggagcatat cctggccagt ggcctggtct
     1381 gtgatgtgct gcgtataccg gatgaaccgt cccgtcgctg gtttgacagg gatattctgc
     1441 gggaggttgt tctggatgca cgggatgaaa tccgcagcat ggcggatgcc ctggcaaaaa
     1501 tccggaaatg atgacgctgg cggggaaaaa tctccgcctt ttcctgccgg tccccggaca
     1561 gagcacaaag gatgtcgccg ccggctccgc tttacccggc catgcggggc atggccttgt
     1621 gggttttcag ttatgtggcc tcagcgtcgt gtgcgggctg tgccgtgcct ccatcgtagc
     1681 cgggcttctg cgggcgggtt gcaaggtaag gtgaaccgct gcggtcaccc ggtccctcct
     1741 tcccgtctgc cgtgattttc cggtcgttca cccgctcagg tttcgcggtc tcaccctcca
     1801 actcccgtca gccgtgtgcg tcaggctgcg gcttcccttg cgcccttgca accctgcagg
     1861 cccggcaaag atggaggcac ggcaccgccc ggtgtcgcgg aatatgtgaa caacggagga
     1921 aatcccatgc agtatgcgaa acctgtcacc ctgaacgttg aagagtgcga ccgcctttct
     1981 tttctgcctt acctgtttgg caacgatttt ctgtatgccg aggcgtatgt gtacgcgctg
     2041 gcgcaaaaaa tgatgccgga atatcaggga ggcttctggc atttcatccg cctgccggac
     2101 ggtggcggtt acatgatgcc ggatggcgac cgttttcatc tggtcaacgg tgaaaactgg
     2161 tttgaccgca ctgtgagtgc tgatgccgca ggtatcatcc tgacttccct tgtgattaac
     2221 cgccagttgt ggctgtacca cgacagcggt gatgcgggac tgacacacct gtaccggatg
     2281 cgcgatgcgc agttgtggag ccacatcgaa tttcaccccg aatgcaatgc gatttacgcg
     2341 gcactggact gattaacgga cggggcggcg atgccgcccc tgtaaaggag acgagaacat
     2401 gtactgtact gttaaagaaa ttatccgcga tgtactggat acagacgtgc cggacagtga
     2461 atgtgttttt gccgtggtgc tgacccgcgg ggatgtgcgc cacatagccc gggactggaa
     2521 cctgtcagac gatgagctgg aaaccgtcat gcagcggctg gacgatgctt ttgtatatgg
     2581 cgcatgtgac agagttgttt ccgatattgt caatgaactg atggaggaaa agcgcgtaaa
     2641 ccgtcttgta acagtccctg cggtgctgct ggaaaaagtg atggtgatgg ctggtagtga
     2701 aatttatcgc ctccatgctg tagggagcga gaacgggggc gacggtgacg catttgtcag
     2761 ggaagaacgt gagattatgc gggtaatgcg tcaggcgctg gacggggaga acgggtaatg
     2821 aatatcagca cggaaacccg cgaaattctg cgtaattaca gggccgtgat taatgcgcgg
     2881 cgtcgtgaaa tggggcagaa accgctcacc accgcgcaga ttgttgatga aatctgtgat
     2941 tttgtggcga atcagcaggc ggttttcctc ggtggtcact acatc
//
LOCUS       DAZGSP010000235         2920 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZGSP010000235 DAZGSP010000000
VERSION     DAZGSP010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.237"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            194..919
                     /locus_tag="ACHK74_005179"
     CDS             194..919
                     /locus_tag="ACHK74_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0223821.1"
                     /translation="MLPGCCKNGIVISKILVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            877..1554
                     /gene="bglJ"
                     /locus_tag="ACHK74_005180"
     CDS             877..1554
                     /gene="bglJ"
                     /locus_tag="ACHK74_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFP0223822.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1592..2380)
                     /gene="fhuF"
                     /locus_tag="ACHK74_005181"
     CDS             complement(1592..2380)
                     /gene="fhuF"
                     /locus_tag="ACHK74_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFP0223823.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMERL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2521..2757
                     /locus_tag="ACHK74_005182"
     CDS             2521..2757
                     /locus_tag="ACHK74_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFP0223824.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTS
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            complement(2796..2882)
                     /locus_tag="ACHK74_005183"
     tRNA            complement(2796..2882)
                     /locus_tag="ACHK74_005183"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2846..2848),aa:Leu,seq:cag)
BASE COUNT          769 a          634 c          730 g          787 t
ORIGIN      
        1 ttcaccaggt tatcatttga tgctaacctt ctgacacatg cagtggagtt gttgtgcagc
       61 aggagtatgc tgatatgaaa gtgaatgcta aggataattt attcgcttaa tctattaatt
      121 tgctggaaac atttaaggaa tgaatatcct gaataaacca tcatgatttt tagacagtgg
      181 atgtggagga aatatgttgc caggatgctg caaaaatgga attgttatca gtaaaatact
      241 tgttatgcaa gcagggttaa aagaggtcat gaggactcac ttccctgaat atgaaataat
      301 atccagcgcc tctgcggagg accttacctt attacaatta cgtcgttccg gattagtcat
      361 tgctgattta gccggtgaaa gtgaagatcc tcgttctgtt tgtgaacatt attattcttt
      421 aatctcacaa tatcgggaaa ttcactgggt tttcatggtg tcacgctcct ggtattccca
      481 ggcagtagaa ttgctcatgt gccctacggc gacgttattg tctgatgttg aacccattga
      541 gaatctggtc aagaccgtac gttccggcaa tacgcacgca gagcgtatca gcgccatgct
      601 gacctctccg gcaatgaatg aaactcatga ttttagctat cgctccgtca ttctcactct
      661 ttcagagcgc aaggtactgc ggctattagg taaaggatgg ggcatcaacc agatagcttc
      721 gttgcttaag aaaagtaata aaactatcag cgcccaaaaa aacagtgcga tgcgtcgact
      781 ggcaattcac agcaacgctg aaatgtatgc atggataaat agcgcgcagg gtgcaagaga
      841 acttaacttg ccttctgttt atggagatgc cgcagaatgg aacacagccg aattaagaag
      901 agaaatgtcg cactcataga aaaatgcgtc atgagcagta tcggtattga gagtttattc
      961 agaaagtttg cgggtaaccc ttataagctc catacctata ccagtcagga gtcatttcag
     1021 gatgccatgt cgcggatctc atttgcggcg gtcatttttt ctttttctgc catgagaagt
     1081 gagcgcagag agggattatc ttgcctgact gaactggcga ttaagtttcc gcgtacccgg
     1141 cgtttagtta ttgcggatga tgatattgaa gctcggctga ttggttcatt gtcgccatca
     1201 ccgctggacg gtgtattaag taaagcgtca acgctggaga tttttcatca ggaactcttt
     1261 ttgtcattaa atggtgtacg tcaggcgacc gaccgactga acaatcagtg gtacattaac
     1321 caaagccgga cgctaagccc gacggagaga gaaatattgc gctttatgtc gcgtggctac
     1381 tcaatgacac aaattgccga gcagcttaaa cgcaatatca aaacgatccg tgcacataaa
     1441 tttaatgtga tgtcgaaact gggcgtcagt tctgatgcag ggttgttgga ggccgcagat
     1501 attctgttat gtatgcggca ttgcgaagca agtaatgtgt tgcatcccta ttaatccgca
     1561 tgatgccggg tttacttccc cggcagtgct ttcatttcag cgtacaatcg ccacattgct
     1621 gcacatccgg taagcgataa cgctggcagc aggtccggcg caccagcagg ccgtcgcgta
     1681 gcaccacggt acgccagagt ggattatctt caccggtagt aagcgttttc tcaaaaaaga
     1741 gggcatggcg cagcgattca accgtcgcct cgccgagcag ttgcttcatc tcagtaagat
     1801 accagttgat caaataaccg gtattactcc agataagttt gccgttgatc tctccggtcg
     1861 cttctagtgc ctgcacaacc ggaaccagcg cctggctgat taacctttcc attcgctgct
     1921 gcggcgaatg tggtgttgcg tttttatctt cacacacatc gacccagaaa caggcggcac
     1981 ggccggtttc gtgaaactca gcatggaaat gttccggcga cacatctaat gccttttcct
     2041 gcgtcagtag cgccagcatt aatggtggca ccatcaggcc gatataccat tgtgcccata
     2101 gtgagatcag cggtttgttc tcgcggatca tcgttggttg gttgcgatag atatgatcgg
     2161 aatagaccgc gagtagagaa cttagcgcat tcggtgatga ccattgcgcc agcgtcatgg
     2221 cgttaagcgg agcgggttca tccaggcgga taaactccag caaatgttca cgatgttcgg
     2281 cgatcgtcgc acgtacggct tgtgcaagcc cagcatcctg tggctggaga tgcgttcgcc
     2341 agataacatc ttcatagagc ggtgcggaac gataggccat aatcgggata gtaatctaaa
     2401 tgataatgat tgctaatcct agcgataggt ttacccgata gcaagggatt tctctggctt
     2461 gcaaatgata aaaattatca tatgatattg gttatcatta tcaatgaaag agatgaaacc
     2521 atgttgcaac gtacgctggg cagtggctgg ggagtgttgc tgccaggatt gctgattgca
     2581 gggctgatgt atgcggattt atcaccggat cagtggcgga ttgtcattct gatgggatta
     2641 gtattgacgt cggtgatgct gtatcacaaa cagttacggc attacgtttt gctaccatcg
     2701 tgcctggcgc ttattgctgc catcatgctg atgataatga atttgaatca gggatgaaaa
     2761 atcaaggaag aaacaagaaa ggaagtaaag ataattggtg cgaggggggg gacttgaacc
     2821 cccacgtccg taaggacact aacacctgaa gctagcgcgt ctaccaattc cgccaccttc
     2881 gcacagtcat cttacttttt ttgatatcgc ctcgtttggt
//
LOCUS       DAZGSP010000236         2887 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZGSP010000236 DAZGSP010000000
VERSION     DAZGSP010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.238"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>2887
                     /locus_tag="ACHK74_005184"
     CDS             <1..>2887
                     /locus_tag="ACHK74_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0223825.1"
                     /translation="YLQFADDYTVTKSNDSSWSGGGIIVNYGTTVKWGINGVSGDDLH
                     KVGDGTLIINGTGKNEGGLKIGAGTVILEQKAKNNDSTAFSSINISGGNSRVKLSGDN
                     QIIPDNVSWGFRGGYLDINGKDTEFSRLQAVDYGAAIINSSTDKSLLTLNLSPLKKDE
                     IAVSVKALDMNAIFQGGHGTAGDLYKTTFYGPTQYYLLKKPKFGSVLMGSLKNTSEWQ
                     FAGTDLNQAVDMAKNNKLTSSAQASYLYHGKLLGNMDIVIPELTGNDILTLDGSVSIS
                     GDMSKQDGALIFQGHPVIHAGQTVSASQSDWENREFSLNNLNLNNADFSLSRNAFMNG
                     NIRAVNQSTVIIGGDTVFTDKNDGTGNDVISVEGKSAAAGTSSYTGHITLEQKSALDI
                     RDNFRGGVTSEDSHINVSSSSVLFSDASSFINSSLNIHKGGALTAQGGLFTSGSIDIG
                     DASLLLTGTPVNSDDAAFLPTINMADGGFKLMSDSSVLKARDQASVVGDIISDKQATI
                     SFGTESGKEGILSEKASRGLAVGLLSGFNTAYRGAIHAPSASATMNNTWWQLTGDSSL
                     RSLKNTGSMPYFTGSAANKAFHTLTVDELTTNGTAYAMRTDLKNADKLVVNQKLSGKD
                     NILLVDFLNKPTGEKLDIELVSAPGNSSKDVFKGSEQAIGFSNVTPVITAKDAGDKTT
                     WNLTGYRMAENPAATQSASGLASVGYKSFLSEVNNLNKRMGDLRDINGEAGAWARIMS
                     GTGSAGGGFSDNHTHVQVGVDKKHELDGLDLFTGFTVTHTDSSASADAFKGKTKSVGA
                     GLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYSTHSWYVGAEAGYRCHVTE
                     DTWIEPQAELVYGAVSGKQFAWKDQGMHLSMRDRDYNPLIGRTGVDVGKSFSGKDWKV
                     TARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSVGLNAEI"
BASE COUNT          825 a          597 c          746 g          719 t
ORIGIN      
        1 gatatcttca gtttgctgat gattatacag taacaaagag caatgacagt tcatggtccg
       61 gaggaggcat tattgtcaat tatggtacaa ctgtaaaatg gggtataaac ggggtttccg
      121 gagatgacct tcataaagtt ggggacggaa cgcttattat taatggaacc gggaaaaatg
      181 aagggggact aaagattggg gctggtactg taatccttga acaaaaagcg aaaaacaacg
      241 attcaacggc tttttcttcc attaacatca gtgggggaaa ctcgcgcgtt aaactttcag
      301 gagataatca gattatacct gataatgttt cctggggatt ccggggggga tatttagata
      361 taaacggaaa agacactgag ttctcccggt tacaggctgt tgattatggg gctgcaatta
      421 ttaacagcag cacagacaag tcattattga cgttaaatct gtcaccattg aaaaaagatg
      481 aaatagctgt aagcgtgaag gcgttagata tgaacgccat ctttcagggg ggacacggga
      541 cagccggaga tttgtataaa acaacttttt acggtccgac acagtattat ctgctgaaaa
      601 agcccaaatt tggcagtgtg ctgatggggt cactgaaaaa caccagcgaa tggcaatttg
      661 caggaacaga cctgaatcag gccgttgata tggcaaagaa taataaactg acatcaagtg
      721 cacaggcttc atatctttat cacggtaagc tcctggggaa tatggatatt gtcattccgg
      781 agcttacagg caatgatatt ttaacgcttg atggctctgt cagtatatcc ggggatatgt
      841 caaaacagga cggtgctctt atcttccagg gacacccggt tattcatgca gggcaaactg
      901 tttctgcatc gcagagtgac tgggagaaca gggagttctc actcaacaat ctgaatctta
      961 ataatgcgga cttcagtctg tcccgtaatg catttatgaa cgggaatatc agggccgtta
     1021 accagagcac tgttattatc ggcggagata cagtctttac tgataaaaat gacggaacag
     1081 gtaatgatgt catcagtgtt gaagggaaat ctgctgccgc aggaacatcc tcctatacag
     1141 ggcatatcac tctggagcaa aaatcagcac tggatatccg cgataatttt cgtggcgggg
     1201 ttacgtctga agacagtcat atcaatgttt cttcatcttc agtcctgttc tcagatgcat
     1261 cgtcatttat aaacagctcc ctgaatattc ataaaggagg tgcgctgacc gctcagggag
     1321 ggctgtttac aagtggaagc attgatattg gtgacgcttc ccttctgctt accggtacac
     1381 cagtgaattc agatgatgct gcttttttac cgaccatcaa tatggctgat ggcggattta
     1441 aactgatgtc tgattcatca gtactgaaag ccagagacca ggcatctgtt gttggtgata
     1501 ttatttctga taaacaggcc acaatcagct tcggaactga atcaggtaaa gagggcatat
     1561 tgtctgagaa ggcatcccgg ggactcgcgg taggattact gagtggtttt aatacggcat
     1621 accgcggtgc aattcatgcc ccgtcagcat ctgccactat gaacaacacc tggtggcaac
     1681 tgacaggaga ctcctcactt cgctcgttaa aaaataccgg aagcatgcca tattttacag
     1741 gaagtgcagc gaataaagca ttccatacac tgacggttga tgagctgacg acgaatggca
     1801 ctgcgtatgc catgcgtacg gacctgaaaa atgcggataa gctggtagta aaccaaaagc
     1861 tgtcaggtaa ggacaatatt ctgctggttg attttctgaa caaacccacc ggagaaaaac
     1921 tggatattga actggtgagt gcaccgggga acagcagtaa ggatgttttt aaaggaagtg
     1981 aacaggcaat aggtttcagc aatgtcacac ctgttattac agctaaagac gccggagata
     2041 aaacaacatg gaacctgacc gggtacagga tggcagaaaa tcccgccgca acccaaagtg
     2101 cctcaggcct tgcatctgtg gggtacaaat catttttgag tgaggtcaac aacctgaata
     2161 aacgtatggg tgacctgcgt gacatcaatg gtgaagctgg cgcatgggca cgtatcatga
     2221 gcggaaccgg ctctgccggt ggtggtttca gtgacaacca cacacatgtt caggtcggtg
     2281 tcgacaaaaa acatgagctg gacggactgg atttgtttac cggcttcact gtcacacaca
     2341 ctgacagcag tgcctctgct gatgctttca aaggtaaaac aaaatctgtg ggggccggac
     2401 tctatgcttc cgccatgttt gattccggtg cctatatcga cctgattggt aagtatgttc
     2461 atcatgataa tgagtacacc gcaacctttg ccggactcgg aacccgtgat tacagtacgc
     2521 attcatggta tgtcggtgct gaagcaggct accgctgtca tgtcactgag gatacctgga
     2581 ttgagccaca ggcagaactg gtttacggtg ctgtatccgg taaacagttt gcatggaagg
     2641 accaggggat gcatctgtcc atgagggaca gggactacaa tccgctgatt ggtcgtaccg
     2701 gtgtggatgt gggtaaatcc ttctcaggta aggactggaa agtgacagcc cgtgccggtc
     2761 tgggctacca gttcgacctg ctggctaacg gcgaaactgt attgcgggac gcatcaggtg
     2821 aaaaacgtat caaaggtgaa aaggacagcc gtatgctgat gtccgttggc ctgaatgcag
     2881 aaatcag
//
LOCUS       DAZGSP010000237         2868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZGSP010000237 DAZGSP010000000
VERSION     DAZGSP010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.239"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..457)
                     /locus_tag="ACHK74_005185"
     CDS             complement(<1..457)
                     /locus_tag="ACHK74_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP0223826.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVKPSDGEGTVISATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKAD"
     gene            719..1054
                     /locus_tag="ACHK74_005186"
     CDS             719..1054
                     /locus_tag="ACHK74_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFP0223827.1"
                     /translation="MKWIVIDTVIQPSCGISFSAIWSKIKLIIWYQSDVFLPPESIFT
                     LTHTGIVLNNKVLPVTIYNVVPFNKRFWDLIKNSQECPANSDNVLKECFNNHCILQIC
                     PYGLKQQCP"
     gene            complement(1356..1520)
                     /locus_tag="ACHK74_005187"
     CDS             complement(1356..1520)
                     /locus_tag="ACHK74_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFP0223828.1"
                     /translation="MTVKLRLAAVALLLFLVVMVDFTSRIMSVLADGVLVVGIVVVLF
                     PLVKKDMPGS"
     gene            complement(1517..1948)
                     /locus_tag="ACHK74_005188"
     CDS             complement(1517..1948)
                     /locus_tag="ACHK74_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
                     /protein_id="HFP0223829.1"
                     /translation="MFGIFKKKTRRAAAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATITGQLDTNFKIGRRAALREIEDVKHDTRE
                     AEDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
     gene            complement(2118..2194)
                     /locus_tag="ACHK74_005189"
     tRNA            complement(2118..2194)
                     /locus_tag="ACHK74_005189"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2158..2160),aa:Arg,seq:tct)
     gene            complement(2208..2284)
                     /locus_tag="ACHK74_005190"
     tRNA            complement(2208..2284)
                     /locus_tag="ACHK74_005190"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2248..2250),aa:Arg,seq:tcg)
     gene            complement(2294..2369)
                     /locus_tag="ACHK74_005191"
     tRNA            complement(2294..2369)
                     /locus_tag="ACHK74_005191"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2334..2336),aa:Ile,seq:cat)
     gene            complement(2410..>2868)
                     /locus_tag="ACHK74_005192"
     CDS             complement(2410..>2868)
                     /locus_tag="ACHK74_005192"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP0223830.1"
                     /translation="LPNESDYLKLQALFARVAEEKHQRGELEKPHHQLLETYTSLNRQ
                     YAELQSEYKHLRRYFGVTAQVPYTDVWTHKPVQYYPGKHPCEKPAEMLQQIISASSRP
                     GDLVADFFMGSGSTVKAAMALGRRATGVELETERFEQTVREVQDLASQNG"
BASE COUNT          763 a          798 c          608 g          699 t
ORIGIN      
        1 ggtccgcttt cggatggtta agacggctca tgtcctgcac atcatgcaga caatggtccg
       61 ccggaataat gtcgttatac ttacaggcgg caccgcccgg tgtcaccgta ctgcggcgcg
      121 ccagctgctt aatacgcggg tccggacggt catatgtctc cggcagcgga aggccttcac
      181 catacgacat gccgtttgac tgccctgcca gaaccacaac aaagtaatac tccgggtcac
      241 tggtggcgct gattactgtg ccttctccat ccgacggctt caccaccaca ggtgtggtga
      301 catcaccttc cgccgcaatg gcctgcatca gggtataagg cgtgatggcg acagggctgc
      361 caaatggctg ccacccctcc ttcagttttt gagtcagtcg ctccgcaagg tctgacggcg
      421 acgccgccct gaccacgtca tagtgtttaa atgccatggt tctttccacc atattaaaaa
      481 caattcttta aaatacctga catgaaatac agaaaaaaaa acacaaaacc ataccttaaa
      541 tgaaaacctc atcataaagc aggtatgcat ggataaacta caagacggga tataaaaacc
      601 tgtatttaaa taaacaataa acaacatcag aaaaataatt ctgctctatg gtttacattc
      661 aaaattatca tttatacttt tcagaacatc accagcacag cataaataag gaggctaaat
      721 gaagtggatt gtgattgata cagttatcca gccatcatgc ggaatatctt tttcagccat
      781 atggagtaaa ataaaattaa taatctggta tcaatcggat gttttcttac ctcctgaaag
      841 tatatttacg ttgactcaca caggtatcgt acttaataac aaagtgctac ctgtaaccat
      901 ttacaacgtt gtaccattca ataaaagatt ctgggattta atcaaaaaca gccaggaatg
      961 tcctgcaaat tcagataacg tattgaaaga atgctttaat aatcattgca ttctgcaaat
     1021 atgcccttac ggactaaaac aacagtgtcc ataattagtt tactcatatc ttacaaaatt
     1081 catataaaag cccctccgga gaggggctaa agccgcgtat ctgtatcatc atgcgcatga
     1141 tgccgggtgc ctcccggtga gttcagcccg gtgccaccaa acccgcgcgt ttactttcct
     1201 gggtatcaaa gataacacct aatgcaccag tcgcccctcc gctcaggggg attcaccatg
     1261 cctgtttctt ttaacaaact ccccgcaaaa cagacaactg tcaaccgtct gaattgtgag
     1321 acatttaaaa ttttcggggc atgactgata cccggctaac tacctggcat gtcctttttc
     1381 accaaaggaa aaagcaccac cacaataccg accaccagca ctccatccgc cagcaccgac
     1441 atgatcctgc tggtgaaatc caccatcacc accagaaaca gcaggagtgc cacagcggcc
     1501 agacgcagtt ttaccgtcac aggtggttct ccagtcgcag gccaagaaca ccagcaatct
     1561 cttccagaac cttacgctct tccggctcaa tttcaccatc tgcttcagca atggccaccg
     1621 ccacatccag cacatcttcc gcttcacgcg tatcgtgttt cacatcttca atttcacgca
     1681 acgccgctcg acgaccaatt ttaaagttgg tgtcaagctg acctgtgatc gtggcactga
     1741 tagcattaat ttctgacgta aacgcggaca acgctggctg gttacgcaag acctgctcga
     1801 tcttcgcttt ctctgaagcc tcacattcac catctgcata ggctaccagg taggcagcat
     1861 taataaccgc ctgtgccaga tcacgtttct caaacttttt aatttccgct gctgctctgc
     1921 gggttttctt tttgaaaata ccaaacatcg tgacgttcct ttaggtgggt gagccaacgc
     1981 ccgggagcga tctgcccaca gagaaagtca cactgaccac tccgtaagct cacccccgaa
     2041 aggctctgtg gttgatatgc gccgggcgtg gcgcggatac aaaaaaggcc cgcaaaagcg
     2101 agcctggtaa ataaatatgg cgcgttgtac tggattcgaa ccagtgaccg attgcttaga
     2161 aggcaattgc tctgtccggc tgagctaaca acgctgaata ccgataatgg accgccatcg
     2221 aggactcgaa ccccgcgcaa ccagcttcga aggctgacgc tctatcccga tgagctaatg
     2281 gcggtatgtg atatggtggc ccttgctgga tttgaaccag cgacctggcg attatgagtc
     2341 gctcgctctc accactgagc taaagggccg agtgcaggat aataacggta cgtaactaat
     2401 cctgctatat catccgttct gactggctaa atcctgaact tccctgaccg tctgctcaaa
     2461 acgttcagtc tccagctcaa caccagttgc acgacgcccc agtgccatcg cggctttgac
     2521 tgtcgaaccc gaccccatga aaaaatctgc aaccaggtcc cccggacgac tgctcgcgct
     2581 gattatctgc tgcagcattt ctgccggttt ttcgcacgga tgtttcccgg gatagtactg
     2641 caccggttta tgcgtccaca catcggtgta cggcacctgc gccgtcacgc caaaataccg
     2701 ccgcagatgc ttatattcac tctgcagttc cgcatactgc cggttcagtg aagtatacgt
     2761 ctccagcagc tggtggtggg gcttctccag ttcaccccgc tgatgcttct cttctgccac
     2821 ccgggcaaac agcgcctgta atttcagata atcactttcg ttcggcag
//
LOCUS       DAZGSP010000238         2847 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZGSP010000238 DAZGSP010000000
VERSION     DAZGSP010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.240"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..289)
                     /locus_tag="ACHK74_005193"
     CDS             complement(<1..289)
                     /locus_tag="ACHK74_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP0223831.1"
                     /translation="MTVRISGVLKDGTGKPVPGCTIELKARRTTETVIVTTVAYGQPG
                     ETGSYSMDVEPGLYRVTLNTEGYAPSYVGDILVKADSAPGTLNKFLMDLEDA"
     gene            complement(433..573)
                     /locus_tag="ACHK74_005194"
     CDS             complement(433..573)
                     /locus_tag="ACHK74_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0223832.1"
                     /translation="MSQKPLKTTVICITVVLIIWITHTSLCEFRFRIAGAEIAAFLQC
                     KQ"
     gene            complement(775..>2847)
                     /locus_tag="ACHK74_005195"
     CDS             complement(775..>2847)
                     /locus_tag="ACHK74_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP0223833.1"
                     /translation="GRILSIDAASRTLTLDREVTLPETGTSTVNLINGSGKPVRVDIT
                     AQPAPDRIQVSILPDGVATYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPE
                     KEAIVDNGARFEPMSGSLNSVIPPAVQHLTVEVSASDGQYLALAKWDTPRVVKGVRFS
                     LRLTSGSGENSRLVTSAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTTFRIN
                     APAAPDSIELTSGYYQITAVPVLAVYDPTVQSEFWFSEKRITDMAQVETFARYLGTGS
                     QWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLDFFRGEIGKTHLA
                     QGMWELIDNSQLDDEMAEMKTTITETRNEITQTVSKTLEDQSATIQQIQRVQTDTNND
                     LAALYMLKVQKTKNGIPYVAGIGAGIEDADGQPLSNILLQADRIAMINPENGNTTPLF
                     VAQGNQLFMNDVFLKRLFAVSITSSGNPPTFSLTPEGRLTARNADISGHISANSGTLN
                     NVTIAENCVVKGTISADRIIGDIVKGYSFEPRDGTLHITNHKYGEGYVIFKAEVSLAD
                     DVSFDRKLILLPFFFYSGTLTIEINGRVVIKAEPRIADTGFSGRFDRNGNAVNTYTNL
                     GDTVSGVWDVVAGGGDTRITVTMRVEGGPGNMGDVEGRKRELVRWRYDAFTLMVAKST
                     DSGFTRRS"
BASE COUNT          570 a          876 c          702 g          699 t
ORIGIN      
        1 gtgcatcctc caggtccatc agaaatttat tcagcgttcc cggtgccgaa tccgccttca
       61 ccagaatgtc acccacatat gacggcgcgt acccttccgt gttcagcgtc acccggtaca
      121 accccggctc aacatccata ctgtaactgc cggtttcccc cggctgacca tacgccaccg
      181 tggtgacaat caccgtctcc gttgtgcggc gcgctttcag ctctatcgtg catcccggta
      241 ccggttttcc cgtaccatcc ttcagcacac ccgatattct gactgtcatg gatttccccc
      301 ataaaaaaaa ccgcagtacc ggtttcccgg cctgcggtaa aatttgcggt ttgttggtgt
      361 taaaacggtg ccatccggct gaccaccctc agcaaccggt cggcggggga tattctcccc
      421 cgccacggtt tcttactgct tacactgtaa gaacgccgca atctccgcgc ccgctatccg
      481 gaaccggaac tcgcacagtg aagtgtgggt gatccagata atgagcacta ccgtgataca
      541 aatcacggtg gtttttaacg gtttttgcga cataaacgct tgctcctttt acggagaggc
      601 gctaaccttt cacttgctta ggatgaatgg tcaggcctcg ggttaaacat gaatgtttgt
      661 ccggggcctt ttcacatccg gccttcaggt gttccctccg gccatcagcc aaaggcaccc
      721 gcgcatactg tacggttttt gtctccttcc ggcaatccgt attccggctg cctgtcacga
      781 tctccgggta aaaccggaat ctgtcgattt cgccaccatg agagtaaagg cgtcatatct
      841 ccaccgcacc agctctctct ttctcccttc cacatccccc atattgccag gaccaccttc
      901 aactctcatt gtcactgtaa tccgtgtatc tccccctcct gccaccacat cccatactcc
      961 tgaaacggtg tccccgaggt tggtgtacgt gttaacagca ttgccatttc tgtcaaaacg
     1021 acctgaaaaa cccgtatcag caattctcgg ttctgcctta atgacaactc ttccgttaat
     1081 ctctatcgtc agtgttcctg aatagaaaaa gaagggaagg agtatcagct tgcgatcaaa
     1141 cgaaacatca tcagcaagag atacctccgc cttaaagatg acataaccct ctccatactt
     1201 gtggttcgta atatggagcg tgccatcacg aggttcgaag gaatagccct tgacgatatc
     1261 accgatgatt ctgtctgcgc taatggttcc tttaacaacg cagttttctg caatggtcac
     1321 gttattcagc gtgcccgagt tcgcactgat atgtccgctg atgtcagcat tgcgggccgt
     1381 cagcctgccc tccggcgtca gggagaacgt cgggggattg ccggacgagg tgatgcttac
     1441 tgcaaacagt cgcttcagga acacatcgtt catgaacagc tgattcccct gcgccacaaa
     1501 cagcggcgtg gtgttgccgt tctccgggtt aatcatcgcg atacggtccg cctgcagcag
     1561 tatattgctc aggggctggc catcagcatc ctcaatcccc gcacctatac cggcaacata
     1621 cggaatgccg ttttttgttt tctgcacctt cagcatgtac agcgcagcca ggtcgttatt
     1681 tgtgtctgtc tgcacccgct gtatctgctg tatggtggcg ctctggtctt ccagggtttt
     1741 actgaccgtc tgcgtgattt cattgcgggt ttctgtgatg gtggtcttca tctccgccat
     1801 ctcatcgtca agctggctgt tatcaatcag ctcccacatc ccctgtgcca gatgtgtctt
     1861 cccgatttct cctctgaaaa agtccagata cccttccgca tcattgctcg cccggccact
     1921 ggcttccaca aacgcagatt tccccaccag gttgacgctg cgcacataaa accagaaatc
     1981 cttccccggc ttaatgtgcg ggccggagac actccactgg ctgcctgtcc ccagataacg
     2041 ggcaaaggtt tccacctgtg ccatgtccgt gatgcgtttt tctgagaacc agaattcaga
     2101 ctgtaccgtc gggtcataca ccgccagcac cggtactgct gttatctgat aataccccga
     2161 cgtcagctca atgctgtccg gtgctgccgg ggcgttaatc cggaacgtgg tggtggcagg
     2221 ttcgccctgc tggccgtagc tgtttatcgc cctgaccgtc agggtgtatt cccccagagg
     2281 caggccactg aaacggtgct ccgtgtcggc agtgatggcg ctggtcacca ggcggctgtt
     2341 ttcaccactg ccactggtca ggcgcagact gaagcgcacg cccttcacca cccgcggcgt
     2401 gtcccatttc gccagcgcca gatactggcc gtctgaggca ctcacctcca ccgtgaggtg
     2461 ctgcactgcc ggcgggatga cgctgttcag tgagccggac atcggctcaa agcgggcccc
     2521 gttatccacg atggcttctt tttccggtac gtgctgcact gccgtgatgg caaaggtgcc
     2581 gtccgtgttt tcccggatgg aaacacagcg gaacaggcga cgacgcagtg acggcaggga
     2641 gagtccccac acaccgtatg tcgccacgcc atcaggcaga atgctgacct gtatccggtc
     2701 aggggcgggt tgtgcagtga tgtccacgcg caccggctta ccgctgccgt taatcaggtt
     2761 caccgtcgat gtccctgttt ccggcagtgt cacctcacgg tccagcgtca gggtacggct
     2821 ggcggcatcg atggacagga tacgtcc
//
LOCUS       DAZGSP010000239         2844 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZGSP010000239 DAZGSP010000000
VERSION     DAZGSP010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.241"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            95..1543
                     /locus_tag="ACHK74_005196"
     CDS             95..1543
                     /locus_tag="ACHK74_005196"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HFP0223834.1"
                     /translation="MSEVPLIAVVMAGGTGSRLWPLSREHYPKQFLQLSGESSLLQST
                     LLRLSTLSCETPLVITNEQHRFVVAEQLRQIDQLRDNIILEPCGRNTAPAIALSAFTA
                     LKRNEQQDPILLILAADHIISKADVFCDAIQKSKTVAEQGNIVTFGIIPEYAETGYGY
                     IKKGDPFKILPNITHDKFYHVQQFIEKPNHSTAEEYVSTGEYLWNSGMFMFKASVYLE
                     ELKKHRPDIYEICEQTASLSYNDLDFIRLPKHIFQDCPSESIDFAVMEKTKNCIVRPV
                     DIGWSDVGSWHSLWDISDKTQDGDVCKGDILTYNTKNNYIYSESALVAAVGVEDIVIV
                     QTKDAILVSKKTEVQDVKKIVEMLKEHERSEYISHREEFRPWGKFDAIDQGERYKVKK
                     IVVKPGEGLSLRMHHHRSEHWIVLSGTAKVTLNDKTVLLAANESIYIPLGATYSLENP
                     GIIPLNLIEVSSGDYLGEDDIVRQKERYRIDD"
     gene            1547..>2844
                     /gene="cpsG"
                     /locus_tag="ACHK74_005197"
                     /gene_synonym="manB"
     CDS             1547..>2844
                     /gene="cpsG"
                     /locus_tag="ACHK74_005197"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFP0223835.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQITLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDVIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLADPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRL"
BASE COUNT          821 a          615 c          680 g          728 t
ORIGIN      
        1 ctgctcgcca gtgaaaatgt tcacgctaac agccgtgttt attttaataa tgatccacgt
       61 gcaatcattg gcttaaataa aaaggaagtc aaaaatgtct gaagtacctt tgattgctgt
      121 cgtaatggct ggtggtacgg ggagccgcct ttggcctctg tctagagaac actatcctaa
      181 gcaatttttg caactatcag gtgagagttc tttattgcaa tcaactttat tacgactgtc
      241 aactctttcc tgcgaaactc cattagtaat aacaaatgag caacatcgct ttgtggttgc
      301 agaacaatta cgtcaaattg atcagttaag agataatatt attcttgagc catgtggaag
      361 aaatacggct cccgccatag ctctttcagc atttacagct ttgaagcgta atgaacaaca
      421 ggatcctatt ttgttgattc tcgcagctga ccatattata agcaaagcgg atgttttttg
      481 tgacgctatt caaaaatcga aaactgtagc tgaacagggg aatattgtaa cctttgggat
      541 tatacctgaa tatgcggaaa caggttatgg ttatataaaa aaaggagatc cttttaaaat
      601 tttaccaaat attactcatg ataagtttta tcatgtccaa caatttattg aaaaacctaa
      661 ccattcgacg gcagaagagt atgtttccac aggagaatat ctctggaata gtggaatgtt
      721 tatgtttaaa gcatcagtat atttagagga attgaaaaag cacaggcctg atatatacga
      781 aatctgtgag cagacagctt cattatccta taacgatctt gatttcatac gattgcctaa
      841 acatattttc caagattgtc cgtcggaatc aattgatttt gcggttatgg aaaaaacaaa
      901 aaattgcatt gttcgtccag tagacatcgg gtggagtgat gtaggctctt ggcattcttt
      961 gtgggatatt agtgataaaa cgcaggatgg agatgtctgt aaaggtgata ttctaactta
     1021 taacactaaa aacaattata tatattctga atcagcccta gtggctgctg taggtgtaga
     1081 agatatagtt attgttcaaa caaaagatgc tattctcgtg tccaaaaaaa ctgaggttca
     1141 ggatgtaaaa aaaatagtag aaatgcttaa ggaacatgag cgctcagagt atatatcgca
     1201 tcgagaggag tttcggcctt ggggaaaatt tgatgcgata gatcagggag aacgatataa
     1261 agttaaaaag atagttgtga aacccggcga aggactatcc ttacgaatgc accatcatcg
     1321 ttctgagcat tggattgttc tttccggcac agcaaaagta actttaaacg ataaaacagt
     1381 attgcttgct gcaaatgaat caatctacat tcctctagga gcaacgtata gtcttgaaaa
     1441 tccaggaata atccctctga atcttattga ggttagttca ggcgattatt taggtgagga
     1501 tgatattgta cggcaaaaag aacgctacag aatagatgac taatatatga aaaaattaac
     1561 ctgctttaaa gcctatgata ttcgcgggaa attaggcgaa gaactgaatg aagatatcgc
     1621 ctggcgcatt ggtcgcgcct atggcgaatt tctcaaaccg aaaaccattg tattaggcgg
     1681 tgatgtccgc ctcaccagcg aaaccttaaa actggcgctg gcaaaaggtt tacaggatgc
     1741 gggcgtcgat gtgctggata ttggcatgtc cggcaccgaa gagatctatt tcgccacgtt
     1801 ccatctcggc gtggatggcg gcattgaagt taccgccagc cataacccga tggattataa
     1861 cggcatgaaa ctggtgcgcg aaggggctcg cccgatcagc ggtgataccg gactgcgcga
     1921 cgtccagcgt ctggcagaag ccaacgactt cccccccgtc gatgaaacca aacgcggtcg
     1981 ctatcagcaa attaccctgc gtgacgctta cgttgatcac ctgttcggtt atatcaatgt
     2041 caaaaacctc acgccgctca agctggtgat caactccggg aacggcgcag cgggtccggt
     2101 ggtggacgtt atcgaagccc gctttaaagc cctcggcgca ccggtggaat tgatcaaagt
     2161 gcacaacacg ccggacggta atttccccaa cggtattcct aacccgctac tgccagaatg
     2221 ccgcgacgac acccgcaatg cggtcatcaa acacggcgcg gatatgggca ttgcctttga
     2281 cggtgatttt gatcgctgct tcctgtttga cgaaaaaggg cagtttattg agggctacta
     2341 cattgtcggc ctgctggcag aagcattcct cgaaaaaaat cccggcgcga agatcatcca
     2401 cgatccacgt ctctcctgga acaccgttga tgtggtgacc gccgcgggcg gcactccggt
     2461 gatgtcgaaa accggacacg cctttattaa agaacgtatg cgcaaggaag acgccatcta
     2521 cggtggcgaa atgagcgccc accattactt ccgtgatttc gcttactgcg acagcggcat
     2581 gatcccatgg ctgctggtcg ccgaactggt atgcctgaaa ggaaaaacgc tgggcgaact
     2641 ggtgcgcgac cggatggcag catttccggc aagcggtgag atcaacagca aactggcgga
     2701 ccccgttgag gcgattaacc gcgtggaaca gcattttagc cgtgaggcgc tggcggtgga
     2761 tcgcaccgat ggcatcagta tgacctttgc cgactggcgc tttaacctgc gctcctccaa
     2821 caccgaaccg gtggtgcgcc tgaa
//
LOCUS       DAZGSP010000240         2799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZGSP010000240 DAZGSP010000000
VERSION     DAZGSP010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.242"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            7..639
                     /locus_tag="ACHK74_005198"
                     /pseudo
     CDS             7..639
                     /locus_tag="ACHK74_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
     gene            complement(658..1161)
                     /locus_tag="ACHK74_005199"
     CDS             complement(658..1161)
                     /locus_tag="ACHK74_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFP0223836.1"
                     /translation="MRSPKVKFLTIFTLSILITKMSFASSACFNEAGTMFRIEPNLIK
                     AIALVESNLKKDSIGKNRDKKNNIKSFDYGLMQINQMHIPMLKKRGIIKDERDLLDNP
                     CLNIKIGTEILYKHFSRCGMTWQCLGTYNAGFAMDNQKKRLQYAQKIYIVYTRLNELD
                     NRKALAK"
     gene            complement(1246..1464)
                     /gene="ygeI"
                     /locus_tag="ACHK74_005200"
     CDS             complement(1246..1464)
                     /gene="ygeI"
                     /locus_tag="ACHK74_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeI"
                     /protein_id="HFP0223837.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDKDQIQAF
                     IVSTWMASFQNDMYSEDNPISPYHKIEW"
     gene            complement(1631..>2799)
                     /gene="ygeH"
                     /locus_tag="ACHK74_005201"
     CDS             complement(1631..>2799)
                     /gene="ygeH"
                     /locus_tag="ACHK74_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFP0223838.1"
                     /translation="LTRCIYSLRCIFEKIGYDRCIETIYRKGYRFSGQVFKTKINEDN
                     TSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAATNFCNDHISQNS
                     FLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDELHNTSQFIIENI
                     LQTVHNPQRSVRLTKQDQGYKNHYLSDEMLAGKKELYEFTPESIYRAMTIFDGLQNKS
                     DIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDITTVDGKILAIMGL
                     ITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVNFHNEKIEEARICIDKSLQLEPR
                     RRKAVVIKECVDMYVPNPLKNNMKLYYKETESESHRVIIDNILKLKQLTRICMR"
BASE COUNT          858 a          446 c          505 g          990 t
ORIGIN      
        1 gaatatatgg gaaaaattaa aattgtagtt tcagatcagc agccgtttat gattgatggg
       61 ataattggat ttctcggaca ttatcctgat ttatatgagg ttgttggggg ctataaagat
      121 ctgaagaaag ctatagcgga gtgtaacaaa tcttcagcct aaatatttat tttaggagaa
      181 tttgcaggtg gaatgatggg gtccgaactc gtaaaatggg ttaaatcgca taagatagat
      241 gctcatatta ttacatttgt agcaaagatg ccgtatattg attcaataaa attgcttgaa
      301 gcaggtgcga aaggatgcgt atggaaaacc agtcacccgg cgaaactaaa tcgtgctatt
      361 gattcgatta gtaatggtta cacttatttt gatagtgtac atatggattg tgaaaaaata
      421 tcttccaggt attcctctga taatcaactt acaaatcgtg agtcagaaat attacaactc
      481 atagctgatg gtaaaacaaa caaagaaatc gctaatttct tgcagttaag caggaaaaca
      541 gtcgaaactc atagactcaa tatcatgaag aaattagatg ttcacagtgg tatcgagttg
      601 atcaaaacag ctttacgtat gggtgtgtgc actatataaa aaaaagaggc tttaggctca
      661 ttttgctaaa gccttacgat tgtctagttc attaagcctg gtatatacaa tatatatttt
      721 ttgggcatat tgcagtcgct ttttttggtt atccattgca aaacccgcat tataagtccc
      781 caggcattgc cacgtcattc cacaacggga aaaatgctta tataaaatct cggttcctat
      841 tttaatgttc aggcagggat tatctaacaa atctcgttca tcttttatta ttccacgttt
      901 ttttaacatg ggaatgtgca tctggtttat ctgcattaaa ccataatcaa aactctttat
      961 attgtttttc ttatctctgt tcttccctat actatccttt tttagattgc tttcaacaag
     1021 agcgatagct ttaatcaaat ttggttcgat tctgaacatt gttcctgctt cattaaagca
     1081 agcgctactg gcgaaagaca tttttgtaat taatatagaa agcgtgaaga tggttaagaa
     1141 tttaactttt ggtgagcgca tatatcttta tgagtcagga ttgtataaaa ggaacgagat
     1201 actattataa acagtatctc gttaataatt tatttggtca atcgtctacc attcaatttt
     1261 atggtaaggt gagataggat tatcttctga atacatatca ttttgaaaag acgccatcca
     1321 tgttgaaact ataaatgctt gtatttgatc tttatcagta gcactggtgt tgatgtgttt
     1381 atctaatgaa acgacttcat cacccgttgc attcgctata tttgaacttt gagataaatt
     1441 attgataccg attggattag tcataaagct ccctttttaa ttgtccaggc caatggtctt
     1501 tacattatac ttttaactct agagcatttt aaatatcttg caataaggta aatatcttag
     1561 atattttgat ctttgttgtt taaataacaa gttgcataca tctaaataat aaaattgcgt
     1621 caataaaaat ttatcgcata caaattctcg tcagctgctt taatttcaaa atgttgtcaa
     1681 ttataactcg atggctttca ctctcagttt ctttatagta gagtttcatg ttgtttttga
     1741 gcgggttagg cacatacata tctacacatt cttttatcac aactgctttt cgtcttctgg
     1801 gttcgagttg tagtgatttg tctatacaaa tccttgcctc ttcaattttt tcattatgaa
     1861 agttgacaag tgccctatag tagtagagag aggctatatc agttgagtgt atcttagcct
     1921 gttcaaataa gatatgagat acttttgctt gtccagacag accagttatc agtcccataa
     1981 tagctaaaat ttttccatcg acagtggtta tgtctgatac ataatctaaa agctctaatg
     2041 ctttttgagc agcaagttca agttcacttt ttccatgaag tgccaaagac atatggcatt
     2101 ccgctagaag gcaataacat tctgttttta gcgtctgtat atcactttta ttttgtaatc
     2161 catcaaatat agtcatggcc ctgtaaatgc tttcaggggt gaactcgtaa agttctttct
     2221 ttccggctaa catttcatct gataaataat gattcttata tccttggtcc tgcttagtta
     2281 atcttacaga tcgttgtgga ttatgtactg tttgaaggat attttctata ataaattgtg
     2341 atgtattatg tagttcatca acaggtagat gattactggc gatgaggaaa aggtttttag
     2401 cgtcgatcaa ctcaatgtat aaagtgttca ctgcattatt ctggtttatt cttcctgtaa
     2461 cgaaataatc tggcttgaat ctgctcaaga atgaattttg agatatgtga tcattacaaa
     2521 aatttgtcgc agccatcgga taggtataga gaccatcgat ttttttattt gaaatgattt
     2581 gcactaattc ctgattaagt attaatggat ccagtgtatt caatgaagta gtgaaaggga
     2641 atatagctat ggaatagtct gaagtattat cttcattaat tttagttttg aaaacctgcc
     2701 cgctgaaacg ataacctttc cggtagattg tttctatgca acgatcatag ccaatttttt
     2761 caaaaatgca gcgcaaagaa tagatacatc ttgtcaggg
//
LOCUS       DAZGSP010000241         2763 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZGSP010000241 DAZGSP010000000
VERSION     DAZGSP010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.243"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..289)
                     /locus_tag="ACHK74_005202"
     CDS             complement(<1..289)
                     /locus_tag="ACHK74_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP0223839.1"
                     /translation="MTVRISGVLKDGTGKAVPGCTIELKARRTTETVIVTTVAYGQPG
                     ETGSYSMDVEPGLYRVTLNTEGYAPSYVGDILVKADSAPGTLNKFLMDLEDA"
     gene            complement(432..572)
                     /locus_tag="ACHK74_005203"
     CDS             complement(432..572)
                     /locus_tag="ACHK74_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0223840.1"
                     /translation="MSQKPLKTTVICITVVLIIWITHTSLCEFRFRIAGAEIAAFLQC
                     KQ"
     gene            complement(772..>2763)
                     /locus_tag="ACHK74_005204"
     CDS             complement(772..>2763)
                     /locus_tag="ACHK74_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP0223841.1"
                     /translation="GRILSIDAASRTLTLDREVTLPEAGTSTVNLINGSGKPVRVDIT
                     AHPAPDRIQVSALPDGVETYGVWGLSLPLLRRRLFRCVSIRENTDGTFAITAVQHVPE
                     KEAIVDNGASFEPLSGSLNSVIPPAVQHLTVEVSASDGQYLALAKWDTPRVVKGVRFS
                     LRLTSGSGENSRLVTTAITADTEHRFSGLPPGEYTLTVRAINSYGRQGEPATTTFRIN
                     APAAPASIELTPGYYQITAVPVLAVYDPTVQYEFWFSEKRITDMAQVETSARYLGTGS
                     QWSVSDPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLGLFREKIGKLHLA
                     QGMWELIDNSQLDDEMAEMKTTITETRNEITQTVSKTLEDQSATIQQIQRVQTDTNND
                     LAALYMLKVQKTKNGIPYVAGIGAGIEDADGQPLSNILLQADRIAMINPENGNTTPLF
                     VAQGNQLFMNDVFLKRLFAVSITSSGNPPTFSLTPEGRLTARNADISGHISANSGTLN
                     NVVIAENCTINGTLKAENIIGDLVKCAGVAFPVDGSYLANGTRTLTVYDDHSFDRQII
                     IPPIIYVGSKQESRTSNDIWTECFLHVDQNGRRIYSGRSVTEPGIFSGIIDMPAGHGH
                     ITLSFTVSSRRQNGSFGSSRISNLQAIVVKKNSAGISIR"
BASE COUNT          538 a          848 c          709 g          668 t
ORIGIN      
        1 gtgcgtcttc caggtccatc agaaatttat tcagcgttcc cggtgcagaa tccgctttca
       61 ccagaatgtc acccacatat gacggcgcgt acccttccgt gttcagcgtc acccggtata
      121 acccgggctc aacatccata ctgtaactgc cggtttcccc cggctgacca tacgccaccg
      181 tggtgacaat caccgtctcc gttgtacggc gcgctttcag ctctatcgtg catcccggta
      241 ccgcttttcc cgtaccatcc ttcagcacac ccgatattct gactgtcatg gatttccccc
      301 ataaaaaaac cgcagtaccg gtttcccggc ctgcggtaaa atttgtggtt tgttggtgct
      361 aaaacggtgc cgtccggctg accaccctca gcaaccggtc ggcgggggat attctccccc
      421 gccacggttt cttactgctt acactgtaag aacgccgcaa tctccgcgcc cgctatccgg
      481 aaccggaact cgcacagtga agtgtgggtg atccagataa tgagcactac cgtgatacaa
      541 atcacggtgg tttttaacgg tttttgcgac ataaacgctt gctcctttta cggagaggcg
      601 ctaaccttcc acttgttgaa ggtaggaacg tcaggcctcg ggttaaacat gaatgtttgt
      661 ccggggcctt cgtctttccg gccttcaggt gttccctccg gccatcagcc aaaggcaccc
      721 gcgcataatg tacggttttt gtctccttcc ggcaatcccg gggcgcgatg ttcagcggat
      781 gctgatcccc gcgctgtttt tcttcaccac tatcgcctga aggttactga tacgtgaact
      841 gccaaaactc ccgttctgac gtcgtgaact tacggtaaaa ctcagggtga tatgaccatg
      901 accggctggc atatcgatga tcccgctgaa aattcccggc tctgtcactg acctgccgga
      961 ataaatccgg cgtccgttct gatcaacatg caggaagcac tctgtccaga tgtcattact
     1021 ggtacgggat tcctgttttg acccgacata gattatcggc gggattataa tctgccggtc
     1081 aaagctgtga tcgtcataca ccgtcagcgt tcgtgtaccg ttcgcaaggt aactaccatc
     1141 caccggaaaa gccacccctg cacatttcac aagatcacca ataatgttct ccgctttcag
     1201 cgtgccattt atcgtacagt tctccgctat cacgacatta ttgagcgtgc ccgagttcgc
     1261 actgatatgt ccgctgatgt cagcattgcg ggccgtcagc ctgccctccg gcgtcaggga
     1321 gaacgtcggg ggattgccgg acgaggtgat gctcactgca aacagtcgct tcaggaacac
     1381 atcgttcatg aacagctgat tcccctgcgc cacaaacagc ggcgtggtgt tgccgttctc
     1441 cgggttaatc atcgcgatac ggtccgcctg cagcagtata ttgctcaggg gctgaccatc
     1501 agcatcctca atccccgcac ctataccggc aacatacgga atgccgtttt tcgttttctg
     1561 caccttcagc atgtacagcg cagccaggtc gttatttgtg tctgtctgca cccgctgtat
     1621 ctgctgtatg gtggcactct ggtcttccag ggttttactg accgtctgcg tgatttcatt
     1681 gcgggtttcg gtgatggtgg tcttcatctc cgccatctca tcgtcaagct ggctgttatc
     1741 aatcagctcc cacatcccct gtgccagatg cagttttcct atcttttccc gaaacagccc
     1801 cagataccct tccgcatcat tgctggcccg gccactggct tccacaaacg cagatttccc
     1861 caccaggttg acgctgcgca cataaaacca gaaatccttc cccggcttaa tgtgcgggtc
     1921 ggagacactc cactggctgc ctgtccccag ataacgggca gaggtttcca cctgtgccat
     1981 gtccgtgatg cgtttttctg agaaccagaa ttcatactgt accgtcgggt catacaccgc
     2041 cagcaccggt actgctgtta tctgataata ccccggcgtc agctcaatgc tggccggtgc
     2101 tgcaggcgca ttaatccgga atgtggtggt ggcaggttcg ccctgccggc cgtagctgtt
     2161 tatcgccctg accgtcaggg tgtattcccc gggcggcagg ccactgaaac ggtgctccgt
     2221 gtcggcagtg atggcggtgg tcaccaggcg gctgttttca ccactgccac tggtcaggcg
     2281 cagactgaag cgcacgccct tcaccacccg cggcgtgtcc catttcgcca gcgccagata
     2341 ctggccgtct gaggcactca cctccaccgt gaggtgctgc acagccggcg ggataacgct
     2401 gttcagtgag ccggagagcg gctcaaagct ggccccgtta tccacgatgg cttctttttc
     2461 cggtacgtgc tgcactgccg tgatggcaaa ggtgccgtcc gtgttttccc ggatggaaac
     2521 acagcggaac aggcgacgac gcagtaacgg cagggagagt ccccacacac cgtatgtctc
     2581 cacgccatcc ggcagggcgc tgacctgtat ccggtccggg gcggggtgtg cagtgatgtc
     2641 cacgcgcacc ggcttaccgc tgccgttaat caggttcacc gtcgatgtcc ctgcttccgg
     2701 cagtgtcacc tcacggtcca gcgtcagggt acggctggcg gcatcgatgg acaggatacg
     2761 tcc
//
LOCUS       DAZGSP010000242         2723 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZGSP010000242 DAZGSP010000000
VERSION     DAZGSP010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.244"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(59..1249)
                     /gene="aspC"
                     /locus_tag="ACHK74_005205"
     CDS             complement(59..1249)
                     /gene="aspC"
                     /locus_tag="ACHK74_005205"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFP0223842.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(1434..2522)
                     /gene="ompF"
                     /locus_tag="ACHK74_005206"
     CDS             complement(1434..2522)
                     /gene="ompF"
                     /locus_tag="ACHK74_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFP0223843.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT          688 a          666 c          648 g          721 t
ORIGIN      
        1 tgttgcgttt gtcatcagtc tcagcccgct tttcagcggg cttcattgtt tttaatgctt
       61 acagcactgc cacaatcgct tcgcacagcg gagccatgtt atctggtgtc atcccggcca
      121 catttacgcg accagaagca accgcatata cgccaaactc ttcgcgcaga cgcagcactt
      181 gttcttttgt caggccactg aaggagaaca tgccgttctg tttgatgata aagctgaagt
      241 cgcggtttgc gcctttttcc tgcagcgtat tgacgaacaa ctgacgcata cgctgaatac
      301 gctggcgcat atcagtcagc tcttgttccc aaatcgcacg taacgcatcg ttgctcagga
      361 tggtggcaac aacagaagcg ccgtgtgctg gtgggttaga gtagttagcg cgaatcgccg
      421 ctttcatttg gctgaatgcg cgatcaacgg tttcactgtc ggcagcaacc agagtacaag
      481 cgccaacacg ctcgttgtac aggccaaagt ttttagagta ggaactggca acaatcagct
      541 ctttatgcat agccgcgaaa gcgcgcagtc cttcagcatc ttcttccaga ccacgggcaa
      601 aaccctggta agcgaagtca aacagcggta accagccttt ctcaacggag agttgtgcca
      661 gtgtctgcca ttgttccagc gtagggtcga taccggttgg gttatggcag cagccatgga
      721 acagcactac gtcgccagcc tgagcttcat tcaggctgtt aatcagtgca tcgaagtcaa
      781 gagtgtgatt ttccgcatca taataagcgt attcacgaac ttccagacct gcagagttaa
      841 agacgctctt atggttcggc cagcttgggt tgctcaccca cactcgctta acgctggtat
      901 tttttgccag gaaatcggca gctacgcgta gtgcgccagt gccccccgga gtctgtgccg
      961 tgcgagcacg tttgtcattg atcagggcgc tacctttacc aaacagcagt tcctgagtgc
     1021 agcgaccaaa ttcagggatg ccgtcaatgc cgaggtaatt tttggtggtt tcattttcga
     1081 gcagatactg ttcagccttt ttcacgctgg tcagtaccgg ggttttgccc gtctcatctt
     1141 tatagacacc aatcccgagg ttaattttgc cgggacgttc atcggcacga aacagatcgg
     1201 ccaggcccag aatcgggtcg gcaggagcgg cggtaatgtt ctcaaacatg acgaggttcc
     1261 attatggtta cagaagggaa gtccgctatc agggtaacgg gagatttaca aaattccaac
     1321 tattactgat gaaaacgcag gctgtttttg caagacgtga gattgctctg gaaggtataa
     1381 aaaaaacagg accaaagtcc tgttttttcg gcatttaaca aagaggtgtg ctattagaac
     1441 tggtaaacga tacccacagc aacggtgtcg tctgaaccta cgcccagttt gttgtcagaa
     1501 tcgatctggt tgatgatgta gtcaacatag gtggacatgt ttttgttgaa gtagtaggtt
     1561 gcgcccactt caaagtagtt caccagatca acatcaccga taccttctac gtctttcgct
     1621 ttagatttgg tgtaagcgat ggacggacgc agaccgaaat cgaactggta ttgcgcaact
     1681 aacagaacgt cttgcgtttt gttggcgaag ccgctggtgt ttgtaaattt attagtgatc
     1741 ggcgtagcgt tacgggtttc accgtagttc gctgccaggt agatgttgtt cgcgtcgtac
     1801 ttcagaccag tagcccactg ttcagctttt ttaccgttgc caagaggttg agcttcttgc
     1861 aggttggtac ggtcagctgc accataagca ccaacgatac caaagccttc gtattcgtag
     1921 ctgatagaac cgccaacacc gtcgccgtta gaacggcgtg cagtgtcacg ctcgttttta
     1981 cccaggtact gaacagcgaa gttcaggcca tcaaccagac caaagaagtt ggagttacga
     2041 taggtagcaa cgccgccaac acgaccaacg aagaagtcat cgctgtatgc agtatcacca
     2101 ccaaattctg gcagcatatc ggtgtaaccc agtgcatcat aaaccacacc gtagttacgg
     2161 ccgtaatcga aagaaccaac gtcagcgtat ttaagacccg cgaatgccag acgcgttttg
     2221 ttaccagttt gagcgtcagc gccttcagag ttgttaccct ggaagttata ttcccactga
     2281 ccataaccgg tcagatcgga attgatttga gtttcccctt taaaaccaag acgggcatag
     2341 gtcatgtcgc cattgccacc gtaactgttt tcaccgttac ccttggaaaa ataatgcaga
     2401 ccaacagctt taccgtacag atctactttg ttgccatctt tgttatagat ttctgcagcg
     2461 tttgcagtac ctgctactaa cagagcaggg acgatcactg ccagaatatt gcgcttcatc
     2521 attatttatt accctcatgg ttttttttat gacacctgcc actgccgtca ataagttctg
     2581 tcaataaaaa tttacggaac tattgatgag agtttggtgt ctttatgtgt ctgcaggcat
     2641 ctttccattc aaactaacgt ttcgctaccg tgaaagtgct acaaagataa agatttagtt
     2701 tcaaaaagaa aaaatatgta acc
//
LOCUS       DAZGSP010000243         2709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZGSP010000243 DAZGSP010000000
VERSION     DAZGSP010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.245"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1122
                     /locus_tag="ACHK74_005207"
     CDS             <1..1122
                     /locus_tag="ACHK74_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP0223844.1"
                     /translation="KLNNAVNWVQRRWDGYTASVARSSIRVKNTSNVIICFSTAVQQL
                     NTVLLVILGTYLIHSDDPHNRITMGALIAAVILSGRALAPLGQIASLATRFQQARLAM
                     QGVNDIVRRPVERESQTHYLTVKDIQGEFRFSDVSFSYKPGLPDALCGLSLCIRPGER
                     VGIIGSIGCGKSTLLKMMVGLYRQNRGQITLDGVAMQQLDPCFVRDKVFLLEQNPRLF
                     LGTLRENLQLSRLPGSLSDVDMINAAGRFGFDRFIRHHPRGLDMQTGEGGQGLSGGQR
                     QMVALTRMVLGSPRVVLLDEPTTGLDLQTESVVLKGLSHWSRGRTMVVVTHRLQVLNI
                     VDRIVVMNEGRVMMDGPRDEILKKLSGKQQSGESRAITS"
     gene            1182..2429
                     /locus_tag="ACHK74_005208"
     CDS             1182..2429
                     /locus_tag="ACHK74_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFP0223845.1"
                     /translation="MTEKTERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKSLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDNVPEDLRERESTV
                     YFLRKTELAQSIAGLKQSKALLDREIAMTRPIVREGAMSEVELLRMQRQSAELQLQMD
                     EKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSRLRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKRLPVIPGMIASVDIKT
                     GRKTVFQYLIKPITRMKQALQER"
BASE COUNT          717 a          556 c          785 g          651 t
ORIGIN      
        1 aagctgaata atgcagttaa ctgggtccag cgccgctggg atggatatac agccagtgtc
       61 gcccgctcgt ccatacgggt aaaaaatacc agtaatgtca taatctgctt ttccacagca
      121 gttcagcaac tgaatactgt tctgctggtc attctgggga cgtaccttat tcacagtgat
      181 gacccacata accgaatcac catgggggca ctgattgccg cagttattct ttccggtcgt
      241 gcgctggcac cgcttggtca gatagcatca ctggccaccc gttttcagca ggcccgcctg
      301 gccatgcagg gggtgaatga cattgtccgc cgtccggtgg agcgggaaag tcagacacat
      361 tatctgacag tgaaggatat ccagggagag tttcgtttca gtgatgtctc cttttcttat
      421 aagcccggat tacccgacgc attgtgcggt ctgtcgctct gtatccggcc cggagagcgg
      481 gtgggtatta ttggctcaat aggttgtggt aaatcgacgc tgctgaaaat gatggtgggg
      541 ctgtaccgtc agaacagggg acaaattact cttgacgggg ttgcgatgca gcaactggac
      601 ccctgttttg tccgggataa agtgtttctt ctggaacaga atccccggct ttttctggga
      661 actctgcgtg aaaatctgca gctttcgcgt cttccgggaa gcctgtcgga tgtggatatg
      721 attaatgcag ccggacgttt cgggtttgac cgttttatcc ggcatcatcc ccgcggactt
      781 gatatgcaga caggggaagg cggtcaggga ctgtcaggag gacagaggca gatggttgcc
      841 ctgacgagaa tggtactggg ttctccccgg gtggtactgc ttgatgagcc cacgacaggt
      901 ctggacctgc agactgagtc tgttgttctg aaaggtctga gccactggag ccgtggacgc
      961 acaatggtgg tggtgacaca tcgtctgcag gttctgaata ttgttgaccg tattgtcgtg
     1021 atgaatgaag ggcgggtaat gatggacggt ccccgtgatg aaatactgaa aaaactgtca
     1081 gggaaacagc agagtgggga gagcagagcg ataacttcat gagcgtcaac cggataagaa
     1141 aaaagagcag atatgtttaa cagtttgtgg accgcaggga tatgacagaa aaaacggaac
     1201 ggaataaagc gtcgtcagag gtggtcaggc agtcagacct ggcgctgttg acagatgtac
     1261 aggcagccct tcagcaggaa aagcatcatg cccttttcct gatggtgatt tttctgctgg
     1321 tactggtagt gacgtttgtt atctgggcat ggaacagtcc tctggatgaa gtgacccgag
     1381 gtcaggggag tattattccc ggcagcaggg agcaggttat acagaccctt gacccgggta
     1441 tactgaaatc acttgaggtc agggaaggcg atattgttga gaaaggtcag gtactgctga
     1501 cactggatga tacccggagc tctgccatgc tgagggaaag tgaagcccgg gtgaataacc
     1561 tggaggccgt cagggcaaga ctccgtgcag aagcttattc cgaatcactg acatttccgg
     1621 ataacgttcc tgaggatttg cgggagcggg aaagtacggt ctattttctc cggaagacgg
     1681 agctggcgca gagcattgcc ggtctgaagc agagtaaggc gctgctggac agggaaatag
     1741 caatgacgcg gccaattgtg cgtgaagggg caatgtcgga ggtcgaactg ttacgtatgc
     1801 agcgtcagtc agcggagctg cagttacaga tggatgagaa acagaataaa tacctgacgg
     1861 aagccggtgc tgaactggtg aagacagaag cagagcttgc tcaggcaaaa gagaatatgg
     1921 ctggcagagc cgacccggtt gagcgttcac gtctcagggc ccccctgaga ggtatcgtaa
     1981 aaaatatacg ggtaaacaca ctgggagggg tggtttctgc aggacaggac ataatggaaa
     2041 ttattccgct ggaagaccag ttgctgattg aagcttatat aaatccacgg gatgttgcct
     2101 atgtcagaac agggatgccg gcacttgtaa aactgacggc ttatgactat gccatttatg
     2161 gtggtcttga cggcgtggtg acactggtaa gtccggatac tttacgtgac cagaaacggc
     2221 cgggagattt aaaactggac ccgaatgaag cttattaccg ggtactggtt accaccagta
     2281 ataattatct gacagacagg aacggaaaaa gattgccagt tattcctggc atgatagcca
     2341 gcgtggatat aaaaaccggg cgtaaaacgg tatttcagta tctgattaaa ccgataacca
     2401 gaatgaagca ggcacttcag gaaagataat aaaaaggtct tccggggaaa gtattttaaa
     2461 tcaattttaa acgggcagaa tgatttcact ttattctgcc cgggtcattg cgcatgcctg
     2521 tatgtgattt ggggctgaag ataatataaa tgttatatct gtcaggggaa aataaatctg
     2581 aaaaaagcgg aatgattaag gaggtccggt cagagaaata aattatctgt aatctgattc
     2641 gtttttattt aatgactccc tttagcggat aaaacataag agcatactgg ttttattaac
     2701 ctgaggata
//
LOCUS       DAZGSP010000244         2650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZGSP010000244 DAZGSP010000000
VERSION     DAZGSP010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.246"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..220
                     /locus_tag="ACHK74_005209"
     CDS             <1..220
                     /locus_tag="ACHK74_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223846.1"
                     /translation="ELDERATRLTLSQWEPELVFEVKARRLKLLRMKSAKTESDRVRL
                     QPDMEHLLAGLIAIDAARAAVLCNSGSS"
     gene            354..2117
                     /gene="tssF"
                     /locus_tag="ACHK74_005210"
     CDS             354..2117
                     /gene="tssF"
                     /locus_tag="ACHK74_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP0223847.1"
                     /translation="MDDLTLRYFDAEMRYLREAGKAFAQAHPDRAAMLDLDKAGTPDP
                     CVERLFEGFAFSMGRLRQKIDDDLPELTESLVSMLWPHYLRTIPSLSVVALTPRLSVM
                     KMAETVPAGLEVTSRPVGPGNTVCRYRTTRAIPLNPLAVEKVVMTTEPDGRSVLKIGF
                     DSSELADWSQVDLHRLSLYLAAEAPVSSTLHLMMTKRLAALYLRLPGNDERIRIDGWF
                     SPGGFAEEDRLWPKGDSAFSGYQLLLEYFTFREKFMFVHLNGLENVSLPAGISGFDLE
                     VVLSQSWPADLPVTDDALCLHCVPVINLFTLEADPLIINGLESEYLLRPKRLQDGYTE
                     IYSVDAVTGSGRTGSAEYVPFTSFRHRGGMLRHDAPERYYHTRVKRGVTGMYDTWLIL
                     GGQRWEADRMPERETLSLRITGTNGQLPRRALQSTLLDRCEQVLQTPVSVHNLCKPTL
                     PVYPPTEDCFHWRVMSHLGTGFLNMLSSAEVLRGTLALYNWRDDELNHRRLDAILAVQ
                     HHRIQRFEKGFLLRGLDVEVTLDGNGFAGEGDIHLFGEMLNRFLALYADMNQFNQLTL
                     IVQPEGKCIRWKENHNPRLPG"
     gene            2072..>2650
                     /gene="tssG"
                     /locus_tag="ACHK74_005211"
     CDS             2072..>2650
                     /gene="tssG"
                     /locus_tag="ACHK74_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP0223848.1"
                     /translation="MHPVERKSQSAPARLITRYRKQLPYINFYRFCQLLEQSQPDQPP
                     IGSGWQARQEAVRFCPYPGMGFPASEIKDAVIPEESHLPPTVQVTFMGLYGVTSPLPA
                     HYISDIAQQREGHEAAADFLDIFSHRLITQYYRIWRKYSYPATFEPGGQDKTSQYLLG
                     LARLGIPGCAQNIATPVSRFLALLPLMLLPGRT"
BASE COUNT          576 a          641 c          756 g          677 t
ORIGIN      
        1 ggagctggat gaacgtgcaa cccggctgac cctgtcacaa tgggaacctg aactggtatt
       61 tgaggtaaaa gcccgccgcc tgaaactgtt acgtatgaag tccgcaaaaa ctgaaagtga
      121 cagggtgcgt ctgcagcctg atatggaaca tcttcttgcc gggctcatcg ccattgatgc
      181 tgccagagct gctgtgttgt gcaattcggg aagttcatga gatgtctgaa ttatattcat
      241 gtgtaatctg attaatgaaa tcctgcctgc agttcagttc ggacgattgt ttgaaatatg
      301 gctgaagtca gagcgcgagt ctccggaaca gttagcttta ttctgagaag ataatggacg
      361 atttaaccct gcgttatttt gacgctgaaa tgcgttatct gcgtgaagcc ggaaaggcgt
      421 ttgcacaggc tcatcccgat cgggcggcga tgctggatct cgataaagcg ggtacgcctg
      481 atccctgcgt ggaacgcctg tttgaaggtt tcgccttttc catgggacgg ctgcgccaga
      541 aaatcgatga tgatttgccg gagcttactg aaagtctggt cagtatgctc tggcctcatt
      601 acctgcgtac tatcccgtcc ctgtcggtgg tggcactgac tccccggtta tccgtcatga
      661 aaatggctga aaccgtacca gccggactgg aagtgacttc ccgccctgtt ggccccggaa
      721 atacagtctg tcgttaccgt accacccggg ccataccact gaatccactg gcagtggaga
      781 aggtcgttat gactacggag ccggacgggc gctcagtgct gaaaataggt tttgacagca
      841 gtgaactggc tgactggtct caggtcgatt tgcatcgtct gagcctgtat ctggcagcag
      901 aagccccggt cagcagcacc cttcatctga tgatgacaaa gcgtctggct gcgctgtatc
      961 tgaggctgcc gggaaatgat gagcggatcc gcattgacgg atggttttca cccggtggtt
     1021 ttgcagaaga agatcggctg tggccgaaag gtgacagtgc tttcagtggg tatcagttat
     1081 tgctggaata tttcaccttt cgggaaaagt ttatgtttgt tcatctgaac ggactggaga
     1141 atgtttctct gccggcaggt atttccggat tcgatcttga ggtggtgctg agtcagtcct
     1201 ggccggcaga tttacctgtg acagacgatg ccctgtgtct gcattgtgtg ccggtgatta
     1261 acctttttac gcttgaagcc gatccactca tcatcaatgg gctggagagc gaatacctgc
     1321 tgcgcccgaa gcgtctgcag gatgggtata cggagatata ttcagtggat gcggtgacgg
     1381 gctccgggcg gaccggcagt gcagagtacg tgccttttac cagcttccgt caccgtgggg
     1441 gaatgctgcg ccatgatgca cccgaacgtt attatcatac ccgggtgaaa cggggtgtca
     1501 cggggatgta tgacacatgg cttatccttg gcggtcagcg gtgggaggcg gatcggatgc
     1561 cggagcggga aacgctttca ttacgtatta caggtaccaa tggtcagctt ccccgtcgtg
     1621 cgttgcagag tacgctgctt gatcggtgtg aacaggtact gcagaccccg gtcagtgtac
     1681 ataatctttg taaacccacg ctaccggttt atccgccgac agaagattgt tttcactggc
     1741 gggtgatgag tcatctgggg acaggatttc ttaacatgct gagcagtgcg gaagtgctgc
     1801 gtggcacgct ggcgctttat aactggcggg acgatgaact taatcaccgt cggcttgatg
     1861 ccattctggc tgtacagcat catcgtattc agcgttttga aaagggtttt ctgctccggg
     1921 ggcttgatgt ggaggtgact cttgacggca acgggtttgc cggagaggga gatattcacc
     1981 tgtttggcga aatgctcaac cgtttcctgg ccctgtacgc cgatatgaat cagttcaatc
     2041 agctcacact gattgttcag cctgaaggaa aatgcatccg gtggaaagaa aatcacaatc
     2101 cgcgcctgcc cggttaatta cccgttaccg gaaacagctt ccgtatatta atttttaccg
     2161 cttctgtcag ttgctggagc aaagccagcc agaccagcct cccattggca gtggctggca
     2221 ggcccgtcag gaagcggtcc gcttttgtcc atacccggga atggggtttc ctgcgagtga
     2281 aataaaagat gctgtaattc ctgaagaatc ccatctgcca cccactgtac aggtgacgtt
     2341 tatgggactt tacggggtca cgtctccgtt gccggcacac tatatcagtg atattgcaca
     2401 gcagcgggaa ggccatgagg ccgcagcgga ttttctcgat atattcagtc atcgtctgat
     2461 cacccagtat taccgtatct ggcgtaagta ttcgtacccg gccacctttg agccgggtgg
     2521 gcaggataaa acgtctcagt atctgcttgg cctggccaga ctggggatcc ccggatgtgc
     2581 acagaatatt gccacgccgg tatcgcgttt tctggcgtta ttgcccctga tgttgttacc
     2641 cggaagaaca
//
LOCUS       DAZGSP010000245         2650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZGSP010000245 DAZGSP010000000
VERSION     DAZGSP010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.247"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(464..670)
                     /locus_tag="ACHK74_005212"
     CDS             complement(464..670)
                     /locus_tag="ACHK74_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223849.1"
                     /translation="MNKEQSADELSLDLIRVKNMLNSTISMSYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNSKDYSLVVD"
     gene            971..1381
                     /locus_tag="ACHK74_005213"
     CDS             971..1381
                     /locus_tag="ACHK74_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFP0223850.1"
                     /translation="MAQAAIFKEIFDQVRKDLNCELFYSELKRHNVSLYIYYLATDNI
                     HIVLENDNIVLVKGLKKVVNVKFSRNKHLIETSYNKLKSKEITFQQYRENLAKAGVFR
                     WVTNIQEHQRYYYAFDNSLLFTESIQKTTQILPR"
     gene            complement(1533..1706)
                     /gene="gnsB"
                     /locus_tag="ACHK74_005214"
     CDS             complement(1533..1706)
                     /gene="gnsB"
                     /locus_tag="ACHK74_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFP0223851.1"
                     /translation="MNIENLKTKAEADISEYITKKIIELKKKTGKEVTSIQFTAREKM
                     TGLESYDVKINLI"
     gene            complement(1878..2108)
                     /gene="ynfN"
                     /locus_tag="ACHK74_005215"
     CDS             complement(1878..2108)
                     /gene="ynfN"
                     /locus_tag="ACHK74_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HFP0223852.1"
                     /translation="MKKIIVFFNSEPAVVVPAMTGVNTIMREYPNGEKTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAIRLLS"
     gene            complement(2113..2178)
                     /gene="ynfR"
                     /locus_tag="ACHK74_005216"
     CDS             complement(2113..2178)
                     /gene="ynfR"
                     /locus_tag="ACHK74_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YnfR"
                     /protein_id="HFP0223853.1"
                     /translation="MKAPSGAFLLGIYSMDTHILR"
     gene            complement(2181..2369)
                     /gene="ynfQ"
                     /locus_tag="ACHK74_005217"
     CDS             complement(2181..2369)
                     /gene="ynfQ"
                     /locus_tag="ACHK74_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP0223854.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            complement(2380..2592)
                     /gene="cspI"
                     /locus_tag="ACHK74_005218"
     CDS             complement(2380..2592)
                     /gene="cspI"
                     /locus_tag="ACHK74_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HFP0223855.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT          862 a          554 c          454 g          780 t
ORIGIN      
        1 taacttttaa ttatcaataa attaccaaac attctgccgc cgccatggaa atgcaaaaac
       61 tagccttttt ccgcgacgct cccgccccgt ggcaggggcc cccaccggga ggacccgaca
      121 gcctgacagc ctgacagccg tgatgagcat ctgatacagc gctttacata atggcatagg
      181 aataattcag aaggacatca cagcatgccc acacaaatta gtgtgagtgt cctgtttctt
      241 cctacgcaca ggactgacga gcatgagggg aaaatatgcg aaccataaat gcctcatctt
      301 cagcaatgca accggcatcc gaacaggacg atattgtaaa ttcacctaat tacgaggaca
      361 ttgcagtagt tgaattgcag ctctgtatta gcctgacagt gacagaatgc gatattgact
      421 ctgtcacagg tgaaatagtt tgaatgatta gcagttatgg tgctcagtca accaccaggg
      481 aataatcctt cgaattctta tcgtgcttca ccaacgctgc ctcaattgcc ctgaatgctt
      541 ccagagacac ctgatgttct atacatgcaa ttacaacatc cgggtaactc atagaaatgg
      601 tgctattaag catatttttt acacgaatca gatccaacga gagttcatca gcagattgtt
      661 ctttattcat tttgtcgctc catgcgtttg ctcgtcatct agcggttgaa atattacttc
      721 aaatcttact gcataaaaga ggaaaagtat gaaagtttga gtacatcgac cttacataca
      781 tctgtcggtt gcatatccct cctggatgcc agcaaggctc aattttgtta cgcaatcaac
      841 actattcatc aaaaacaggc ttaatatttg acataaatca tcaacaaaac acaaagaggt
      901 cagaccagat tgaaacaata aacacgataa tgctaactac gcgccatcgt atcacatgga
      961 aggtttacca atggctcagg ctgccatttt taaagaaata ttcgatcaag tgcgtaaaga
     1021 tttaaactgt gaattatttt attctgagct aaaacgtcac aatgtctcac tttatattta
     1081 ctatttagcc acagataata ttcacattgt gttagaaaac gacaacatag tgttagtaaa
     1141 aggactcaaa aaggttgtaa atgttaaatt ctccagaaac aaacatctta tagagacctc
     1201 ctataataag ttgaaatcaa aagaaatcac atttcaacaa tacagggaaa atcttgctaa
     1261 agcaggagtt ttccgatggg ttacaaatat ccaggaacac caaagatatt actatgcctt
     1321 tgataactca ttactattta ctgaaagcat ccagaaaact acacagatct taccacgcta
     1381 aaccataacg tccggcttct ctcactcctg agccggactg cattggttta ataaaaacca
     1441 tcaacaattg tgatttagat attcggaacc attcaaatat aacaaaaccc cgtaaaaacg
     1501 aggtttatgg ataaatttta ttattgaata catcagatta aattaatctt gacatcatag
     1561 ctttcaagac ccgtcatttt ttcccgtgcg gtaaactgaa tactggtaac ttctttcccg
     1621 gtctttttct taagttcaat aatttttttt gttatatatt cagaaatatc tgcttctgct
     1681 tttgttttta agttttcaat attcatcatt tcctctttta gtctgttatg actttccagt
     1741 tacacagtaa gtcgattata tggtgcaaac gtgtaaaaga taagatgaaa catcgcaata
     1801 atcaacatac gatagtctaa attttacaca aacagacaaa gagaattttc ctgaattatc
     1861 aatacaatag catcaaatca actcaagagc cttattgctg cttccagaat ttcttctgaa
     1921 gtaacatgtc gatccgcggc tacataaatg actttatgat ctccggtcag agatggaaac
     1981 cctgcggcca ttacagtaag gtgtgttttt tcgccatttg gatattcacg catgatggtg
     2041 ttaactccag tcatcgctgg cactaccact gctggttcag agttaaaaaa aactatgatt
     2101 tttttcatga tgttaccgta gtatgtgagt atccatcgaa tagataccaa gcaaaaaagc
     2161 tcccgaagga gccttcattt tcactttttt aaatccaacg acagacggct ggcatttaag
     2221 tattgtgaaa tattatcaaa tgtaatcatc attgatttac aaaagataca ttttgccccg
     2281 aaaggattca tgtcagaaac atcaaaagat gatgttctat actgggaacc atgacaacac
     2341 gggcatctaa agtgaatatg gtttgtaata ttgtctacct caaagcgcca ctacatgaac
     2401 agcggcagga cctttaggtc cgttctcaat accaaattca acttcctgat tctcagttaa
     2461 tgttttgaaa tcgttgctct gaattgctga gaaatggaca aacacatctt tgctgccatc
     2521 tttcggcgtg atgaaaccaa aacctttttc agggttaaac catttcacta aaccagtcat
     2581 tttgttagac ataattatta ccttttgaag aaattagccc ttgggcagaa tggtccgaaa
     2641 aaaaatatca
//
LOCUS       DAZGSP010000246         2557 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZGSP010000246 DAZGSP010000000
VERSION     DAZGSP010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.248"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1002)
                     /locus_tag="ACHK74_005219"
     CDS             complement(<1..1002)
                     /locus_tag="ACHK74_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223856.1"
                     /translation="MLKSFCLNFDGMYLSIVSFLSEKFNLKRVGKMELESKEDYEETT
                     HHNVASSVVTATHDNLDGREFFELCSNPALVFSFETHKKFNELPVDSKESPLDLFIAN
                     TKQLHLLRSGDISNTLSHLLLLGYVSAAESYLRALIRNLINHDEYIQSIVSDKAVSYA
                     AAQHHKKELLPEALLEDFSLASPYNVFETLKDIIGMKGQRPIEMMKCSSEFMKVCELR
                     HCCVHRFGKLGSKNAIRLGLAEHMKHLEKPIILNNDDLEQIAFIVENFIRTLNNTVFK
                     FIINRTVENKNKEKGGERLYDSEWTWVFEKDISRYEKYYAIFSAKNDTLPGLSLQDSY
                     "
     gene            complement(980..>2557)
                     /locus_tag="ACHK74_005220"
     CDS             complement(980..>2557)
                     /locus_tag="ACHK74_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP0223857.1"
                     /translation="SPGIKLIVGPRGCGKTHLMRYAWVECKNDDNAPLPLYVSFNKYF
                     KLEPLLKNKPNAIDLFHKWVLAKILLSAYEIACDIEDSEYLDLSTILIADKEFLINLI
                     VRLEQGLSPSLEQENIVQLISVSSVISSLSSLCDWLERKRVIILLDDAAISLTPEYLY
                     EFFDIVRSLKTSKIALKASVYPGTTEYGPRFHVAHDAETIDAWISVQHPNYSSVMDAI
                     AQARFPGYDGIPDDLKELFKFSAFGIPRSFLMMLRDYQTTLSQTTQQKFNKIIERYVE
                     LRLSEYSSLKDKLPRLFDIVSLGESLLLKVVELLKESNSQYKEEKQVIIGIEKNDNVY
                     FSRLTKLLIETGLFYNLPDISHGDGRTYERYIPHYALLIKERVFNEGSRGFSPSQIIQ
                     KILRKNAKHPVRRNISSLFNAEQLARLKLTLPPCNNCKTPRLTDNQKFCHHCGNQLID
                     ESAYNQCMSIPLSKVPHLTSWQMQKLSGLEKVKTIGDVLSLQDPGSELRKIHRIGPKI
                     ANKIMDKVLLHVEEFLS"
BASE COUNT          809 a          477 c          406 g          865 t
ORIGIN      
        1 atatgaatcc tgtaatgata atccgggaag ggtatcgttt ttggcactaa atattgcata
       61 atacttttcg tatcgactta tatctttttc aaacacccat gtccattcag aatcatatag
      121 tctttcccct cctttttcct tatttttgtt ctcaactgta cgattaatta taaatttgaa
      181 aacggtatta ttcaaagttc taataaaatt ctcaacaata aatgcgattt gctcgagatc
      241 atcattattg agtattatcg gtttttctaa gtgcttcata tgttcagcca atcctagtcg
      301 gattgcattt ttcgaaccca acttaccaaa tcgatgaaca caacaatgcc ttaactcgca
      361 aactttcatg aattcgcttg agcatttcat catttcgatt ggtcgttgtc ctttcattcc
      421 aataatgtcc tttaatgttt caaaaacatt ataagggctt gccaatgaaa agtcctcaag
      481 taaagcttct ggtaataact cctttttatg gtgttgtgcc gctgcataac taacggcctt
      541 atcagaaact atagactgta tatattcatc atgattaatt agatttctga taagagctcg
      601 caaataactt tcggctgcag acacataacc taataacaac agatggctta atgtgtttga
      661 aatatcacca gatcttaaca agtgaagttg ctttgtatta gctataaaaa gatctaacgg
      721 ggattccttg ctatcaacag gtaactcatt aaattttttg tgtgtctcaa acgaaaaaac
      781 tagagctgga ttactacata gttcaaaaaa ctctcttcca tctaagttat catgggttgc
      841 agtgacaact gaggacgcca cattatgatg agtggtctct tcataatcct cttttgattc
      901 tagttccatt ttcccgactc tttttaaatt aaacttttca gataagaaag acacgattga
      961 caagtacatg ccatcaaaat taagacaaaa actcttcaac atgtaataac actttgtcca
     1021 taattttatt ggcaattttg ggtcctattc tgtgaatttt tcttaactca ctcccagggt
     1081 cttgtaaaga caatacatct cctatagttt ttactttttc cagcccagat agtttttgca
     1141 tttgccaaga tgttagatgt ggtacttttg acagtggaat agacatgcac tgattatagg
     1201 ccgactcatc aatcaattga ttcccacaat gatggcaaaa tttttgattg tcagtcaatc
     1261 gcggagtctt gcagttattg catggaggta aagttagttt aagtctcgca agttgctcag
     1321 cattgaataa tgatgaaatg tttcttctga caggatgctt agcattcttt cttagtattt
     1381 tttgaataat ctgagaagga gaaaaacctc tacttccttc gttaaacact ctttccttta
     1441 ttaataaagc ataatgaggt atatatcttt cataagtacg cccatcacca tgagaaatat
     1501 caggaagatt ataaaaaagc cctgtttcaa ttaatagctt agtgagtcga gaaaaataaa
     1561 cattatcatt tttctctata ccgattatga cttgtttctc ttctttatat tgggagttac
     1621 tttccttgag taattcgact acttttaata aaagtgattc acctaatgaa acaatgtcaa
     1681 acaatcgagg tagcttatct tttagtgaac tgtattcgct gagtctaagc tcaacatacc
     1741 tttcaattat tttattaaat ttttgctgag ttgtctgact taaagttgtt tgataatctc
     1801 ttaacatcat caaaaaagaa cgtggaatac caaatgctga gaatttaaat aactctttca
     1861 agtcatccgg aatgccatca tacccaggaa accttgcctg cgctatagca tccatcactg
     1921 aggaataatt agggtgctgt accgaaatcc aagcatcaat tgtttcagca tcatgagcaa
     1981 catgaaacct tgggccatat tcggttgttc caggataaac cgatgccttt aaagctattt
     2041 ttgaggtttt tagactacgt acaatatcaa aaaactcgta aagatattca ggagttaagc
     2101 ttattgctgc atcgtctaat aaaataatga ctcttttacg ttcaagccaa tcacatagtg
     2161 aactcaacga ggatatgaca gaagaaacac tgatcaattg aacaatgttt tcttgttcta
     2221 atgatggcga aagaccctgc tcaagcctta cgattaaatt aattaggaac tctttatccg
     2281 ctatgagtat cgttgacaaa tcaagatatt cagaatcttc aatatcgcaa gcaatttcat
     2341 aagcagagag tagtatcttt gctaataccc atttatgaaa gagatcaatt gcatttggtt
     2401 tattttttaa aagcggctct aacttaaaat atttattaaa agaaacatag agtggtaatg
     2461 gtgcgttatc atcgttttta cattcaaccc aagcataacg catcaaatgt gttttaccac
     2521 atcccctagg accaactata agtttgatgc ctgggga
//
LOCUS       DAZGSP010000247         2554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZGSP010000247 DAZGSP010000000
VERSION     DAZGSP010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.249"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1044)
                     /locus_tag="ACHK74_005221"
     CDS             complement(<1..1044)
                     /locus_tag="ACHK74_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP0223858.1"
                     /translation="MNTSEKQLNNFAVVCGVALRGLLRPLPVTTVEWADQNYYLPPES
                     SYLAGRWKTLPFQVAIMNCMGNDRIRTINLIKSARVGYTKMLMGVIGYFIEHKNRNSL
                     LFQPTDSAAEDFMKSHVESTLRDVPCLKILSPWLGRKHRDNTLTLKRFTSRVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSAAGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALK"
     gene            complement(1016..1564)
                     /locus_tag="ACHK74_005222"
     CDS             complement(1016..1564)
                     /locus_tag="ACHK74_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP0223859.1"
                     /translation="MNVNKKKLAEIFNVDIRTIDRWQTQGLPQISGGGKGVEVIFDSA
                     KAIEWYAQREADIENEKLRKEVEDLRAASESDLQPGTIDYERYRLTKAQADAQELKNA
                     RDSGQVIDTGFCLFALGRLAQEISAILDSIPLSMQRQFPALTPAMLDFLKTDIAKAAN
                     RCASTAEKLPEMLDEYLREAAK"
     gene            complement(2067..2252)
                     /locus_tag="ACHK74_005223"
     CDS             complement(2067..2252)
                     /locus_tag="ACHK74_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP0223860.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPVQAPCVKPPPPPAWIMQPA
                     PDWQTQLNGIISPSESG"
BASE COUNT          634 a          682 c          602 g          636 t
ORIGIN      
        1 tttcagtgca tccagccagt catagactat ctgtacccag gtggtgaacg gactgtacgc
       61 cgtccagata tggaaagtga tggagcgcgg cggcggaatt tcattacccg cagcgctgaa
      121 aaacgtcagg ccgtcacggg tccacatgcc cgtgttttca cagatccacc gcccgttgct
      181 ctggtcaagc tcagactgat ggatcacgca gccatgatgt tcacagagat agaaaacgct
      241 ttcggggctg tccttctccc atttaaggcc aaaaggcgtg gattcatcgc caaatttcag
      301 atactgctcc tccccacagt gcgggcaggg cacataaaaa cgcatgaaat gtgccgactc
      361 gttggccgct ttttcgatct ggcaggagcc ttttattttt ggcgtcgagc cgcgaatgga
      421 ttttggccat accgagccct caatacgctt atcccccagc agggttggcg agccctcttt
      481 ttcgacatcc ggctcgaatg aggaaagttc gtcatagcag accacatcca ctgatttttc
      541 acggtagttt ttggcggcag caccacccag gcaccagaac cccacacggg acgtgaatct
      601 ttttaatgtc agtgtgttat cccggtgctt acgccccagc caaggggaaa gaattttcag
      661 acagggaaca tcccgtaatg ttgactccac atgagacttc ataaaatctt cagcggcaga
      721 atccgtaggc tgaaacagaa gactgttacg gtttttatgc tcaataaagt aaccaataac
      781 gcccatcagc attttggtgt aaccgactcg agctgatttt ataagattaa ttgtgcggat
      841 ccggtcatta cccatgcagt tcattattgc cacctgaaag ggcaacgtct tccaccgccc
      901 agcaagataa gaggactccg ggggcaaata ataattttga tcggcccatt caacggtggt
      961 tacaggcagt gggcgtaaaa gcccacgaag tgccactcca caaacaacag caaagttatt
     1021 tagctgcttc tctgaggtat tcatccagca tctccggtaa cttctctgcc gttgatgcgc
     1081 atcggtttgc cgcctttgct atatcggttt tcaggaaatc aagcatagcg ggcgtcagtg
     1141 ccggaaactg tctctgcatg gacaatggaa tggagtcaag aatggcagag atctcctgtg
     1201 ccagtcttcc cagagcaaaa aggcaaaacc ctgtatcaat gacctgccca ctgtcgcggg
     1261 catttttcag ttcctgtgca tctgcctgtg ccttcgtcag tcggtaacgt tcgtaatcaa
     1321 tggtgccggg ctgaaggtct gattcgctgg ccgccctcaa atcctcgacc tctttacgga
     1381 gtttttcatt ttcaatatca gcttcgcgct gagcatacca ttcgatcgcc ttcgcagaat
     1441 caaagatgac ctcaacacct tttcctccac cagatatctg agggagcccc tgagtttgcc
     1501 agcgatcaat agtgcgtata tcgacattaa agatttcagc aagcttcttt ttattaacat
     1561 tcattcagca attccttatc aaaaatcagt tacgacatga aatgccaaaa aagacagatt
     1621 tttcagcgtt ctgatgtcgt ttctttcttg atgattattt ctttaaaaac aataggttaa
     1681 caacaagaag aactgacatg ctttttcccg aaaaattttc ataaatagcg aaaacccgcg
     1741 aggtcgccgc cccgtaacgg gtccatatgc cggaaaggac ccgagaaaat gataatggtt
     1801 atcagttgca acaaatccag tttcttccac catcgcaccg gacaggcgac tatgagggga
     1861 caacgccgcg ctccgttaac gcggtaaacc ccggtgtgta tcgtttttga ttatccccgc
     1921 acactctcgc agaggagtct ccctgtcggg ctgcggtctc tgttaatacg ggaatacggc
     1981 gacgatacgg cgcatcagca aaacttattt caggcactga gtgcggatat attcctgcgc
     2041 cccttccagc tgcttctgca tcatcatcaa ccgctctctg agggtgaaat aatcccgttc
     2101 aactgtgtct gccagtcggg ggccggttgc attatccacg ccggaggtgg tgggggcttc
     2161 acgcacggag cctggacagg tggcgttgat ccgcaggcgc ttacgaccag cggcaacatc
     2221 agcacgcaga gtttcatttt cagctctcgc atcggctaat tccctcgagt attttgcatc
     2281 gagcgcagca acatcgcgct ggcgctgctg catatcagta atggttgcgt ttgccagctc
     2341 cagctctctg gcttttttat cgcgctgctc tttgtaggtg atggcgttat cgcggtaatg
     2401 attcagcccc agactaagcg caccacaggc caccagcagg gcaatgatga ccacacacag
     2461 aacgcggttc atttcaccac cagcgtatct gaccgatgaa ataaccgggg gccataatca
     2521 caaacaccag ccagataagg atgaacttcc aggt
//
LOCUS       DAZGSP010000248         2498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZGSP010000248 DAZGSP010000000
VERSION     DAZGSP010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.250"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2051
                     /locus_tag="ACHK74_005224"
     CDS             <1..2051
                     /locus_tag="ACHK74_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP0223861.1"
                     /translation="KSRKSVKSVEPEITELANSWLKSYRLDYKLQNASLNGEIDNALD
                     AYFSKNGGAGGNRPDVKLLVKDKYGKQYPVLIEYKGYKDRLIRLGSNGIIENKDARKE
                     WNFKNINGYAVNGAVHYANALLQFTNYPDIIAIGMTGWRDDGTGELHHEIGVWYVSKN
                     NLGAGQKVGEFTDLSFLADKNVDGFLNKIKLLNLPPEELEKIKASKEEEIDTRLSRLN
                     NDIYQNEKGLGESDRVYLVVATVIATLGIPGKLAPLDKKELTSSTEEDLRDGDIIFRK
                     IRNFLRLKAVPETKREMILRSLQNTLWTENINKPVNGESQLKRVFVKVVDDLGEYYKI
                     GLTTDFTGKLFNEMYRWLGFTQDKLNDVVLTPPYVATLLARLARVNKDSYVWDFATGS
                     AGLLVAAMNEMLIDARENIHSPNELQLKEAQIKAEQLLGLEVLSSIYMLAILNMILMG
                     DGSSNILNKDSLADFDGKYGFGKTGEKFPADAFILNPPYSAKGNGMIFVQKALSMMDK
                     GYAAVIIQSSAGTGKATEYNKKILKENTLLASIKMPADLFIGKSSVQTYIYVFQVKIP
                     HNAKQAVKFIDFSNDGYARSNRKKARNNLVDADRAKERYQEVVDLVHFGKGCLNIFTE
                     DEYFEGTIDPDSGEDWNQTRPVDARPTLEDFKKTVGDYLAWEVSQLLKKQGENNFAGK
                     "
     gene            2120..>2498
                     /locus_tag="ACHK74_005225"
     CDS             2120..>2498
                     /locus_tag="ACHK74_005225"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP0223862.1"
                     /translation="MSTGSLISSGRLKPGKIKRVSAKSDQNGIIGEFDTEFMDDARHY
                     ENFISVNFFGDAFYHPYCASVEMKVHVLTLKSSKSDFNAKRGLYVASMINKALKGRFN
                     YGQQLSSSKLRDGEYFISLPVKNN"
BASE COUNT          773 a          458 c          605 g          662 t
ORIGIN      
        1 gtaaatcaag aaagtctgta aaatctgttg agccggaaat taccgaactg gcgaactcat
       61 ggcttaaatc ttacaggctt gattacaagt tgcagaatgc atcacttaac ggagagatcg
      121 ataacgcact ggacgcttat ttttcgaaaa atggcggcgc aggcggtaat cgtcccgacg
      181 taaaactcct tgttaaggac aaatacggca agcagtatcc cgttctcatc gagtataaag
      241 gctacaagga caggcttatc agacttggca gcaacggtat tattgagaac aaagatgcca
      301 gaaaagaatg gaattttaaa aatattaatg gatacgcggt gaatggcgca gtccattatg
      361 ccaacgcact tcttcagttc accaattacc ctgatattat tgctattggt atgaccggct
      421 ggcgcgatga tggtacaggc gaactgcatc atgaaatcgg cgtgtggtac gtatcaaaga
      481 ataatttagg agccggacag aaggttggtg aattcaccga cttatcgttc cttgctgaca
      541 aaaatgttga tggatttctc aacaagataa agctgcttaa tcttcctccc gaagaactgg
      601 aaaaaatcaa ggccagcaaa gaggaagaga tagacacgcg actttcccgt ctgaataacg
      661 atatttacca gaatgaaaag gggcttggtg aaagcgatcg tgtttacctt gttgtggcta
      721 ccgttattgc aacgttgggg attccgggaa agttagcacc gctggataaa aaagaactga
      781 cctcttcaac ggaggaggac ttgcgtgatg gtgatattat tttcaggaaa ataaggaatt
      841 ttttaaggct gaaagctgtt cctgaaacaa aaagagaaat gattttgcgc tcgttgcaaa
      901 atacgctgtg gactgaaaac attaacaaac cagtcaacgg tgaaagccag ttaaagcgcg
      961 tgtttgttaa agttgttgat gatcttgggg agtattacaa aattggactg accaccgatt
     1021 ttacgggcaa actgtttaat gagatgtatc gctggcttgg atttacgcag gacaagctca
     1081 atgacgtcgt tctgacacca ccttatgttg ccacattgct ggcacgactc gccagagtaa
     1141 acaaagattc ctatgtgtgg gattttgcta caggttctgc gggattgctt gtggctgcaa
     1201 tgaatgaaat gctcattgac gccagagaaa atattcactc accgaacgaa ctacaactta
     1261 aggaagcgca aatcaaggct gaacagcttc ttggccttga ggtgttatcc agtatttata
     1321 tgctggccat cctgaatatg attttgatgg gggatggtag ctcgaatatc ctcaataaag
     1381 actcgctggc cgattttgac ggcaagtatg gatttgggaa aacaggagaa aaattccctg
     1441 cggacgcatt tattctcaat cctccgtatt cagctaaagg taacggcatg attttcgtgc
     1501 agaaggcgct gtcgatgatg gataaaggct atgctgcagt gattatccag agttcagctg
     1561 gcacaggaaa ggctacggag tacaacaaaa aaatactcaa ggaaaatacc ttgctggcaa
     1621 gcatcaaaat gcctgcggat ctgtttattg gtaaatcaag tgttcagacg tacatttacg
     1681 tttttcaggt aaaaattccg cataacgcga aacaggctgt taagtttatc gacttttcca
     1741 atgacggcta cgctcgctct aatcgcaaaa aggcaagaaa caatcttgtt gatgccgatc
     1801 gtgcaaaaga gcgttatcag gaggttgtgg atctggttca cttcgggaaa ggttgcctta
     1861 atatctttac ggaggatgaa tattttgagg gaacaatcga tcctgatagt ggtgaggact
     1921 ggaatcagac caggccagta gatgctaggc caacgctgga ggatttcaaa aagacggtag
     1981 gtgattacct tgcatgggag gtctcgcaat tactgaaaaa acaaggtgaa aacaattttg
     2041 cgggaaagta aattccccgc tcagcgataa attaagaagc gttgagtggg gagagttcag
     2101 gattggcgat ttgttctatg tgagcacagg ctcgcttatc tcctcaggaa gattaaaacc
     2161 aggcaaaatc aaacgtgttt cagcaaagag cgatcaaaac ggtatcatcg gagagtttga
     2221 taccgagttt atggatgatg caaggcatta tgaaaatttt ataagcgtta atttttttgg
     2281 agatgctttt tatcatcctt attgtgccag cgtagaaatg aaggtgcatg ttcttacatt
     2341 aaaatcaagt aagtccgact ttaatgcaaa gcgcggactg tatgttgcca gtatgattaa
     2401 taaagcttta aaggggcgtt ttaattatgg tcaacagtta tcgagttcca aactcagaga
     2461 tggagaatat ttcatctcat taccagtgaa aaataatg
//
LOCUS       DAZGSP010000249         2490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZGSP010000249 DAZGSP010000000
VERSION     DAZGSP010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.251"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            65..490
                     /locus_tag="ACHK74_005226"
     CDS             65..490
                     /locus_tag="ACHK74_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0223863.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGIELLPVEITPGEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            487..837
                     /gene="tnpB"
                     /locus_tag="ACHK74_005227"
     CDS             487..837
                     /gene="tnpB"
                     /locus_tag="ACHK74_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP0223864.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGELVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNSLTML"
     gene            868..2481
                     /locus_tag="ACHK74_005228"
     CDS             868..2481
                     /locus_tag="ACHK74_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFP0223865.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDEQLSLVEETEAFLRSAL
                     ARAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPEHLPREINRLEPEESCCPECGGELDYLGEISAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGVELSRALLSNWVDACCQLMTPLNDALYRYVMNTFKLHTDDTPVKVLA
                     PGRKKAKTGYIWTYVRDDRNAGSPEPPAVWFAYSPDHQGKHPEQHLRPFRGILQADAF
                     NGYDRLFSAEREGGALTEAGCWAHARRKIHDVYISTKSATAEEALKLIGELYVIEHEI
                     RGLPVSERLAVRQMQSKALLTSLHKLMQEKEHTLSKKCRLRDAFRYIRKHWVALCNFC
                     DNGLAEVDNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LSHILSVLPEWPSNRVDELLPWNVVLTNK"
BASE COUNT          636 a          629 c          695 g          530 t
ORIGIN      
        1 tatgtagcca tcagacgaga attggtaact tagacgccca tctgacacag acggacatct
       61 aagtatggaa ttacaggact ggcgaaaaga acctcgtaaa aactattcga atgaattcaa
      121 acttcgtatg gtggaactgg catcacaacc cggagcttgt gttgcacaga ttgcacgtga
      181 aaatggcgtc aatgataatg ttattttcaa atggctcagg ctctggcaga acgaagggcg
      241 tgtttcgcgg cgtcttccgg taacgacctc ttctgacact ggcattgaat tattacctgt
      301 agagataacg ccgggtgaac agaaagaacc tgtggcggcc attgcgccgt ctttatccac
      361 ttccactcag accagagtca gtgccagctc ctgcaaggtg gagttccgtc acggtaacat
      421 gacgctggaa aatccatcgc cagagctgct cacggtgttg atccgtgaac tgaccgggag
      481 gggaagatga tctcactccc atcaggtacc cgcatctggc tcgttgccgg cgttaccgat
      541 atgcgtaaat ccttcaacgg actgggagaa ctggtacaac atgtgctgaa tgataatccc
      601 ttctccggtc acctgtttat cttccgtggc cgacggggtg acaccgttaa aattctttgg
      661 gccgatgctg atggtctgtg cctgttcacc aaacgcctgg aggaaggcca gtttatctgg
      721 cctgcggtac gtgacggcaa ggtatccatt acccgttcgc aactggcaat gctcctcgat
      781 aagctggact ggcgtcagcc aaaaacatcc cgccttaact cactgacaat gttgtaaaaa
      841 actcctgacc gcattataaa aacggtcatg agtcagaaat acctcattcg catcgcagag
      901 ctggaaaggt tgctctctga gcaggctgaa gccctccgtc agaaagacga gcaactgagt
      961 ctggttgaag agacggaggc cttcctgcgc tctgcactgg cacgtgccga agaaaagatc
     1021 gaagaagatg aacgggaaat agaacatctg cgggctcaga tagaaaaact gcgccggatg
     1081 ctgttcggta cccgttctga aaaactgcgt cgcgaggttg aacaggctga ggccctgctg
     1141 aagcaacgcg aacaggacag cgatcgttac agtgggcgtg aggatgaccc gcaggttccc
     1201 cgccagctgc gacagtcacg ccatcgtcgt ccgttaccgg agcatctgcc ccgcgaaata
     1261 aaccgcctgg agcctgaaga aagctgttgc ccggagtgtg gcggtgagct ggattatctg
     1321 ggggaaatca gtgcagaaca actggaactg gtgagcagcg ccctgaaagt gatccgcaca
     1381 gaacgggtaa aaaaagcctg tacaaaatgt gactgtattg ttgaagcacc ggcgccgtcc
     1441 cgcccgatag agcgtggtat cgcgggcccc ggattacttg cccgcgtgtt aacgggaaaa
     1501 tactgcgaac acctgccact gtatcgtcag agtgaaatct ttgcccgcca gggcgtcgaa
     1561 ctgagccggg ccttactctc caactgggtt gatgcgtgtt gccagttaat gacgccgctg
     1621 aatgatgctc tgtaccgtta cgtgatgaat accttcaagc ttcacactga cgacacaccg
     1681 gtaaaggtac tggcaccggg caggaagaag gcgaaaacag gatatatctg gacgtatgtc
     1741 cgggatgacc ggaatgccgg ttcgccagaa cccccggcgg tctggttcgc ctactcaccg
     1801 gatcatcagg gtaaacatcc ggagcagcac cttcgtccct tccggggtat cctgcaggca
     1861 gatgcgttta atggttacga tcggctgttc agtgccgaac gtgaaggcgg cgcgttgacg
     1921 gaagcaggat gctgggctca tgcgcgacgc aaaatccacg atgtatatat cagtaccaaa
     1981 agcgcgacag cggaagaagc cctgaaacta atcggtgaac tgtacgtcat cgagcacgaa
     2041 atacgcgggt tgccggtgtc tgaacgcctg gcggtcaggc aaatgcagag taaagcgcta
     2101 ctgacgtccc tgcataagct gatgcaggag aaagaacaca cattatcgaa aaaatgtcgt
     2161 ctgagagatg cgttccggta tatcaggaag cactgggttg cgttgtgcaa cttctgtgat
     2221 aacggtctgg cggaagtgga taataatgcc gcggaaagag cgcttcgtgc agtctgtctc
     2281 ggaaagaaaa actttatgtt cttcggcagc gatcacggcg gcgagcgtgg agcactgttg
     2341 tacgggctga tcggcacctg ccgtctgaac ggtatcgatc cggaagcgta tctgagccat
     2401 attctgagcg tactgccgga atggccttcc aaccgggttg acgaactcct gccatggaac
     2461 gtagtactca ccaataaata agcgtcaata
//
LOCUS       DAZGSP010000250         2487 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZGSP010000250 DAZGSP010000000
VERSION     DAZGSP010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.252"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            97..2364
                     /gene="adiA"
                     /locus_tag="ACHK74_005229"
     CDS             97..2364
                     /gene="adiA"
                     /locus_tag="ACHK74_005229"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFP0223866.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRKQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT          631 a          671 c          613 g          572 t
ORIGIN      
        1 ttccaaaaat gtttgttttt cacgcgcttt acagcccgaa aaggccggaa gatacttgcc
       61 cgcaacgaag attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc
      121 gagtttctcc atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta
      181 agccagcaaa atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc
      241 tcttcaaacg aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa
      301 catcaaaacg tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc
      361 ttcctgttgg gcgatcggga aaaagccctc gccgcaatgg accgggatct gctggagctt
      421 gtcgatgaat tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgccgtt
      481 gccgcgatga cccgctaccg caagcagctg ttgccgccac tgttcagcgc gctgatgaaa
      541 tatagtgaca tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt
      601 accaaaacac ccgccggacg tttctaccat gactactatg gtgaaaatct gtttcgcacc
      661 gacatgggca tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc
      721 gaaagcgaaa aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc
      781 ggtacttccg gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg
      841 gtcgttgacc gtaattgcca taaatccatc gaacaaggct tgatgctgac aggcgcgaaa
      901 ccagtctaca tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag
      961 gaaatgcaac ctgaaacctt gcagaagaaa atcagtgaaa gcccgctgac taaagacaaa
     1021 gctgggcaaa aaccgtctta ctgcgtggtg accaactgca cctatgacgg cgtgtgttat
     1081 aacgctaaag aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa
     1141 gcctggtacg gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc
     1201 gaacctggcg atcacaacgg tcctaccgtt ttcgccaccc actccaccca caaactgctg
     1261 aatgcgctgt cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc
     1321 tcccgcttca accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc
     1381 gcatccaacg acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacacaa
     1441 gaagtgattg acgaagcggt tgattttcgt caggcgatgg cgcggctgta taaagagttc
     1501 accgctgacg gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa
     1561 accggcaaaa cctatgactt tgctgacgca ccaacaaaac tgctgaccac cgttcaggac
     1621 tgctgggtaa tgcatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg
     1681 agtatgctcg acccgattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa
     1741 ctggaagaaa ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt
     1801 gtgcctaccc gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt
     1861 gggaaatggg ggactctggt taacaccctt tgctccttca aacgccacta tgacgccaac
     1921 acaccgctgg cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac
     1981 atggggattc acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc
     2041 gcacggttga acgaagccta ttccggcctg ccggtggcgg aaatcacccc gcgtgaagcg
     2101 tacaacgcga ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc
     2161 atcgcggcaa actcagttat cccatatccg ccaggaatcc cgatgctgct gtctggtgaa
     2221 aacttcggcg ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac
     2281 caccatttcc ctggatttga acacgaaact gaagggactg aaattattga cggtatttac
     2341 cacgttatgt gcgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac
     2401 attacgccgc ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca
     2461 aacaaaaatg tcatactttt tgcgcgg
//
LOCUS       DAZGSP010000251         2479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZGSP010000251 DAZGSP010000000
VERSION     DAZGSP010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.253"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..330
                     /locus_tag="ACHK74_005230"
                     /pseudo
     CDS             1..330
                     /locus_tag="ACHK74_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332514.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(621..1094)
                     /locus_tag="ACHK74_005231"
     CDS             complement(621..1094)
                     /locus_tag="ACHK74_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223867.1"
                     /translation="MKVILATRNRYLEYGLQQMLEGYRVILAREFFIPENRKSVPTHD
                     ESWVIICDAMVSRLMRCMFQGRRYLQLDAEEITGRLETDRKIRNGDWEQNTYARPLTM
                     SEMVVMFGYVYRESKPCHLAREMGIHTKTVNTFLYMGLGKNGLRYRSVKHLVGRA"
     gene            complement(1182..2453)
                     /locus_tag="ACHK74_005232"
     CDS             complement(1182..2453)
                     /locus_tag="ACHK74_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFP0223868.1"
                     /translation="MNKKFSKTILSSVVAGLLLVSSGGVAKEIDIGGKYKVILTEETG
                     SARIVDINTKKDLFDGIINAKTGTVTTVNKKEIQSAIDSFYAANPKINKDDPNIKQMV
                     AQAAGNSGRYTVSLPSGLPSLTTENIFNLKVTDIEKIKSNIDGVNSVITSKTAKDYNI
                     AISNGMSSEAALTAAKDGNGALLQEFNRLGTNVNDLKNATTFALDENGEITYEKGVES
                     IPVKDVVAEVKADTTIRKNKDGSYTLDQTAPDTVRVNAAIVDLDKNLKDRTTVGVNSD
                     GSLTTAEGATKTVAVSEGLVSLSGRTGRIDAAVGGIDTRVTKNTQTIQNHSRQLQEHN
                     ARLNSQQRQIRENHEEMKRAAAQSAALAGLFQPYSVGKFNATAALGGYSDKQAMAVGV
                     GYRFNEQTAAKAGIAASDGDVSYNVGVNFEF"
BASE COUNT          620 a          657 c          479 g          723 t
ORIGIN      
        1 aacaatccta atccatgcgt gatgtgtggt gcctgttgtg catttttccg cgtctctttt
       61 tactgagctg aagcagatga tgctggcggt accatccccg taacactgac agaacagatt
      121 tctccgtacc atcgatgcat gcagagcact aaccagaaaa atcctcgctg tgtggtcctg
      181 agcggtcaac cgagagtcaa tgcctgctac agcatttatg aaaatcgctc atccacctgc
      241 agggaatttc ctatgtccgg caacaatggt gtggttaatg aagcatgtaa ccgtgcgcga
      301 gcgaaatacg acctaacccc actttactaa acgcgatcgg tatcgctgtc atgacatatt
      361 ttgtatgcca ttattctgac gacttaacaa ctcacttatt gtaatcaagt ttctttcttc
      421 tggtcgggga gctgtgcagc tccccttttt tattgcatca ttaaggcagc cactgcacag
      481 agcgttacca ccgtacccag ccacacaaaa agcatgaaac acatcgctcc tgctgtgtag
      541 cgcttcttta cgtatttcag atttgcctgt ttcatgacaa tttaccagat tattcttcac
      601 ccccggataa caaaacgcgg ctatgcccgc cccacaagat gcttcacact tctgtacctc
      661 aggccgtttt taccaagccc catatacaga aaggtgttca ctgtcttcgt gtgaattccc
      721 atttcacggg caagatgaca cggctttgac tcccggtata cataaccaaa catcaccacc
      781 atctctgaca tcgtcagcgg acgtgcgtat gtgttctgtt cccagtcacc gttacggatt
      841 ttcctgtccg tttccagacg ccctgtaatc tcctccgcat ccagttgaag ataacggcgt
      901 ccctgaaaca tacagcgcat cagacgactc accatagcat cacagataat cacccaggac
      961 tcatcatggg tcggtacact cttcctgttc tccgggataa aaaattccct cgccagtatc
     1021 accctgtaac cttccagcat ctgttgcaga ccgtactcca gataacggtt acgggtcgcc
     1081 agaatcactt tcatcgcagc cccctgtttc atgctcacat gagaaaaaaa cggcaggcca
     1141 aatcccctgc ctgccgtcat gcttcatgtc actgtccgca attaaaactc gaagttcaca
     1201 ccaacgttgt aggatacatc accgtcgctg gctgcaatac ctgctttggc tgctgtctgc
     1261 tcgttaaagc ggtaacccac accaacggcc atagcctgtt tgtcactgta accacccagt
     1321 gctgccgtgg cgttgaattt acccacactg tacggctgga acaaacctgc cagtgccgca
     1381 ctctgtgcag cagcacgttt catttcttcg tggttttcac gaatctgacg ctgctgactg
     1441 ttcagccgcg cgttatgctc ctgcagctgg cgggagtgat tctggatagt ctgagtgttt
     1501 ttggtgacac gggtgtcaat gccaccaacc gcagcatcaa tacggccagt tcttcccgac
     1561 agggaaacaa ggccttcact taccgcaaca gtttttgtgg ctccttcagc agtggtcagg
     1621 ctgccgtctg aattcacgcc aacagtggtt ctgtctttca gatttttatc cagatcaaca
     1681 attgcagcgt tcacacgaac agtatcaggc gcggtctgat ccagggtata gctgccatct
     1741 ttgttcttac gaattgttgt atcggctttt acctctgcaa ccacatcctt aacaggaatg
     1801 ctttccactc ctttttcata ggtaatttca ccattttcat caagagcaaa tgtagtggca
     1861 tttttcaggt catttacatt tgtgccgaga cggttgaatt cctgtaacag cgctccatta
     1921 ccatctttag ccgctgttag cgcagcttct gaactcatgc cgttactgat agcaatattg
     1981 taatcttttg cagttttact ggtgataact gaattaacac catcaatatt agattttatt
     2041 ttttcaatat cagtcacttt tagattgaaa atattttcag tcgttaacga agggagccca
     2101 ctgggtaaag agactgtata acgccccgaa ttaccagcag cctgagccac catctgttta
     2161 atgtttggat catctttatt tatttttggg ttagcggcat aaaaactatc tatagcagat
     2221 tgaatctctt ttttgtttac agtagtaaca gtgcctgttt tcgcattaat aattccatca
     2281 aataaatctt ttttagtatt tatatccaca atccttgccg aacctgtttc ctctgtcaga
     2341 ataactttat atttaccacc aatatcaatc tcttttgcaa cacccccact acttaccagc
     2401 aacagtccgg caacaacaga agaaagaata gtcttagaga attttttgtt catctggtat
     2461 accctatgaa atgatttgt
//
LOCUS       DAZGSP010000252         2367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZGSP010000252 DAZGSP010000000
VERSION     DAZGSP010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.254"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..487
                     /locus_tag="ACHK74_005233"
     CDS             <1..487
                     /locus_tag="ACHK74_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223869.1"
                     /translation="CKKMVPFYSDDNCLLCNKKNEELSPTRDEINKIREFDVTISQDN
                     LNSVTQRLLPTPLAAVRARRVAFENPYRNKVLSESLPAGNLLYRSALAFIQALIALVP
                     SKKILDLDKITDALRRRYVHLEQLEYAELRNALACALSTCYNRKYLIKVAKGKYLPVD
                     D"
     gene            709..915
                     /locus_tag="ACHK74_005234"
     CDS             709..915
                     /locus_tag="ACHK74_005234"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223870.1"
                     /translation="MEQGLLPENEKITSVDVEDWTPERRFSVVLETATLSEMELADYY
                     RRMGLFVEQVKEWRAISIQAHEKN"
     gene            1088..1252
                     /locus_tag="ACHK74_005235"
     CDS             1088..1252
                     /locus_tag="ACHK74_005235"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0223871.1"
                     /translation="MERRKIIALVREAVSNGARKSQACRCIGISIRTLQRWTDGNVIT
                     TDVSVNGAPY"
     gene            complement(1256..1723)
                     /locus_tag="ACHK74_005236"
                     /pseudo
     CDS             complement(1256..1723)
                     /locus_tag="ACHK74_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1850..1936
                     /locus_tag="ACHK74_005237"
     CDS             1850..1936
                     /locus_tag="ACHK74_005237"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0223872.1"
                     /translation="MVKLALQPGASVARIAQEHDINDNLLFK"
     gene            complement(2288..>2367)
                     /locus_tag="ACHK74_005238"
     CDS             complement(2288..>2367)
                     /locus_tag="ACHK74_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP0223873.1"
                     /translation="IGFANTTFSDADFGALGILLGIIVR"
BASE COUNT          697 a          527 c          535 g          608 t
ORIGIN      
        1 atgtaaaaag atggtgcctt tttattctga tgataattgt ttgctttgta ataagaagaa
       61 tgaagagttg tcaccaacca gagatgaaat taataaaatt cgcgagtttg atgtaacaat
      121 atcccaggac aatcttaatt cagttactca acgattgtta cccacaccat tagctgcggt
      181 aagagcccga agagttgcat ttgaaaatcc atatagaaat aaagtactaa gtgaatcatt
      241 acctgctgga aaccttcttt acagaagtgc attagctttt atacaggcct tgattgcatt
      301 agtcccatca aagaaaatac tggacttgga taagataaca gatgctctgc gtaggcggta
      361 tgttcatctt gagcaattag aatatgccga attaagaaat gctttagctt gtgcattgtc
      421 aacttgttat aatagaaaat atctcattaa agttgcaaaa ggaaaatatt tacctgttga
      481 cgactaggtg cgtattaccg tgaaggagat cggtgagtaa ccccctgtgt cagcatagtt
      541 gtcgcctcaa ttttttaagg agataacaat gccaggacaa ccaataccaa gtgagctcaa
      601 agagaaggac tgatatggct tttaccacca tactgctggt cacatcgtca gatagcccgt
      661 aagctaaaga tcagtgcctc agttgtatca aaatggagat atgagttagt ggagcaaggg
      721 cttttacctg aaaatgaaaa gattacctcc gttgatgttg aagactggac accggagcgt
      781 cgattctcag ttgttcttga gacggctacg ctgtcagaaa tggagcttgc tgattattac
      841 cgacgcatgg gattgtttgt tgaacaggtt aaagaatggc gggctatctc aattcaggcg
      901 catgaaaaaa attgacggaa aatattcgaa cagacagggc attgcgtgac gcaaaaaata
      961 agttacgaga actgtagaaa gagcttaagc gtaaagataa agctctggcg gaagctgcgg
     1021 ccctgcttat attgagggaa aagttcaatg ccctctggga caacagcgag gaagactgac
     1081 ccctctcctg gagcggcgta aaattattgc ccttgtccgc gaggccgtct ccaacggcgc
     1141 ccggaaaagt caggcttgta ggtgtatcgg tatttcaatt cggacacttc agcgctggac
     1201 tgatggcaac gttattacca cggatgtaag cgtcaacgga gcaccgtatt gacgcttatt
     1261 tattggtgag tactacgttc catggcagga gttcatcaac tcggttggaa ggccattccg
     1321 gcagtacgct caggataagg cgcagatacg cttccggatc gataccgttc agacggcagg
     1381 tgccgatcag cccgtacaac agtgctccac gctcgccgcc gtgatcgctg ccaaagaaca
     1441 taaagttttt ctttccgaga cagactgcac gaagcgctct ttccgcagca ttattatccg
     1501 cctccgccag accgtcatca ctgtaataac agagggcatc ccactgattc agtacatagc
     1561 tgaacgcttc gcccagtctg gattttttcg acagcgtacc attcttctcc accatccatt
     1621 catgcagcga cgtcagtaac actttgcttc gctgctgcct gacggaaaga cgctctgact
     1681 ccggtaatcc ccgtatttcg taagcgtaaa ccgaccgccg tatgtagcca ttaagccagt
     1741 attggtaacg taggtgccca ccttttcaaa ctagtggacg cctgttatgg agcaaaaaac
     1801 attatccgca gaaccccgca gatcattttc aaataatttt aaacttcaaa tggttaaact
     1861 ggctttgcaa ccaggagcct ctgttgcccg tatcgcccag gaacacgata tcaatgataa
     1921 cctcctgttc aaatagctca gactctggca aaacgaaggg cgtatatctc ggcgtcttcc
     1981 ggtaacaacc tcttctgaca ctggagttga attattacct gtggaggtga cgctggatga
     2041 gcggccttac cccgtttttt acgcgaggta attatgaaga tcaatgacta gccgtcgaac
     2101 cgtgtgcatg aactgctgca ctgaaacttc tcagctgtaa aataatcctt acgctacgta
     2161 cttctcggag cgcttacgat taatcggatg cagtcctgac tggaagaaag aaaaatggga
     2221 aaaaataaga ggaattaata tcctccacgt tcctgaaatc ctggaggata ttaatgtcat
     2281 caaaacatta gcgaacgata atccctaaca aaatacccag tgcgccaaag tcagcatcac
     2341 tgaaggtggt gttagcaaaa ccaatat
//
LOCUS       DAZGSP010000253         2362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZGSP010000253 DAZGSP010000000
VERSION     DAZGSP010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.255"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            235..567
                     /gene="hdeA"
                     /locus_tag="ACHK74_005239"
     CDS             235..567
                     /gene="hdeA"
                     /locus_tag="ACHK74_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFP0223874.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            683..1009
                     /gene="hdeB"
                     /locus_tag="ACHK74_005240"
     CDS             683..1009
                     /gene="hdeB"
                     /locus_tag="ACHK74_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFP0223875.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            1073..1720
                     /locus_tag="ACHK74_005241"
     CDS             1073..1720
                     /locus_tag="ACHK74_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFP0223876.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1762..2292)
                     /locus_tag="ACHK74_005242"
     CDS             complement(1762..2292)
                     /locus_tag="ACHK74_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP0223877.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
BASE COUNT          713 a          457 c          518 g          674 t
ORIGIN      
        1 agaagaaaat cccctgctat caatctatgc caaaaacgcg tctaagaatg cagtcgattt
       61 aataaaaatt tcctaattac agtatctgat gcatctgtaa ctcattgtat tgaaataaaa
      121 atatctgatt ttgatatttt ctatcaacat gacatataca gaaaaccagg ttataacctc
      181 agtgtcgaaa ttgattcgtg acggctcttt cactttatag ttgaggatat tacgatgaaa
      241 aaagtattag gcgttattct tggtggtctg cttcttctgc cagttgtaag caatgcagcg
      301 gatgcgcaaa aagcagctga taacaaaaaa ccggtcaact cctggacctg tgaagatttc
      361 ctggctgtgg acgaatcctt ccagccaact gcagttggtt ttgctgaagc gctgaacaac
      421 aaagataaac cagaagatgc ggttttagat gttcagggta ttgcaaccgt aaccccagct
      481 atcgttcagg cttgtactca ggataaacaa gccaacttta aagataaagt taaaggcgaa
      541 tgggacaaaa ttaagaaaga tatgtaattc cgggaatgcg ttacatcgta cttccttgca
      601 tattgaacag gccggaatat cttctttaaa agcagctatt cctcctgttc atatataatc
      661 tctatattga atgggttaca aaatgaatat ttcatctctc cgtaaagcgt ttatttttat
      721 gggcgctgta gcggctttgt cactggtgaa cgcacaatct gcgctggcag ccaatgaatc
      781 cgctaaagat atgacctgcc aggaatttat tgatctgaat ccaaaagcaa tgaccccggt
      841 tgcatggtgg atgctgcatg aagaaacagt atataaaggt ggcgataccg ttactttaaa
      901 tgaaaccgat ctcactcaaa ttcctaaagt gatcgaatac tgtaagaaaa acccgcagaa
      961 aaatttgtat accttcaaaa atcaagcatc taatgacttg ccgaattaat gaggtacaag
     1021 taaaaaggag tagcaagttg agccatcttg ctgctccttt ttgcattttt atatgacagc
     1081 agaatttatt atacgtctta tactcgcggc aattgcctgt ggcgctattg gcatggaaag
     1141 gcaaatgcgc ggcaaaggag cagggttacg cacacatgta ttaattggca tgggaagcgc
     1201 cctgtttatg attgtttcga aatatggttt tgctgacgtg ctgtctttag atcacgtcgg
     1261 actcgacccc agccgtattg ctgctcaggt ggtgacgggc gtcgggttta tcggtgcagg
     1321 taacattctc gttcgtaacc aaaatattgt aggtctgacg acggcagcgg atatctgggt
     1381 gaccgccgcc ataggtatgg ttattggcag cggtatgtac gaactgggta tttatggttc
     1441 agtgatgacc ttgttggtgc tggaagtctt ccatcaatta accttccgcc tgatgaacaa
     1501 aaattaccat ttacagctga cattagtgaa cggtaatacg gtatcaatgc tcgactggtt
     1561 taagcagcaa aaaataaaaa cggatttagt ttcgttgcag gaaaatgaag atcatgaagt
     1621 ggtcgccatt gatatccagc ttcatgccac aacgtcgata gaagacctgt tgcgattatt
     1681 aaaaggtatg gcaggggtga aaggcgtttc aattagctaa tctgtgtggc agcgtagcca
     1741 gactcaccgt aagcctgaaa ttcacaccag ataatcaata tgctgatggc gtaccagctc
     1801 attgatccgc ttaagattga ggatcatcat gatattgtgt ttatggcagt agaacgtttt
     1861 tagcgatatt ttcagtttct ccagaatatc ttccgttgtc atttgattcg caatgcagct
     1921 cagaacctga tgttgcttgc gcgaaagtga cacagccgct tcaggcacag agcagtaact
     1981 gccattgatg agtgcgacaa aatcaatgat agtgatagtc cgcggaacaa aggtcaccgg
     2041 cactttcccc aggcagcgtt taatattaaa gggggagaaa ataatcacat ggacaggttt
     2101 taatcgttca atttgagtga gaaaattaga atgaaataca ttattcatta atgtatcaat
     2161 aatgacgaag gcattctgat gcaacattac gtcgatctct tcttcgttct gtatatgaac
     2221 gacattacct ttactcagaa tgtttttcat cgcggtgaag aacatcgtat ccctggtaat
     2281 tataagaaac ataaccgact cctggtgcgg acattaactt tataatcagt ttgccaaagg
     2341 taattaacag gacagggctt at
//
LOCUS       DAZGSP010000254         2356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZGSP010000254 DAZGSP010000000
VERSION     DAZGSP010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.256"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2117
                     /locus_tag="ACHK74_005243"
     CDS             <1..2117
                     /locus_tag="ACHK74_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP0223878.1"
                     /translation="TLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGHRV
                     AVHYGYDDKGRLTGERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEWLT
                     YGSGYLAGMKLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQSQH
                     LNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYATDP
                     AGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDDER
                     THHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRKRDLTGWMSLSRK
                     PEMTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEKLQ
                     QEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEY
                     TPARKVHFYHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQ
                     HDEESGLYYNRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQL
                     LYDVWHDDSYGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHAG
                     IGGGINAAITYSETKSLPTSGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVGI
                     GAGVDASVMTLACRTWQECWVN"
     gene            2129..2314
                     /locus_tag="ACHK74_005244"
     CDS             2129..2314
                     /locus_tag="ACHK74_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223879.1"
                     /translation="MKKYIFLVLFLLCGCSPVDIEPARQEVNIFRGLYQTGKYSDIYK
                     ITSSDFQTATSEKNLST"
BASE COUNT          603 a          578 c          718 g          457 t
ORIGIN      
        1 tcaccctctg gtactacgat gaatcggacc gtatcactca ccgcacggtg aacggcgaac
       61 cggcagagca gtggcagtat gatgaccacg gctggctgac agacatcagc cacctgagcg
      121 aaggccaccg tgtcgctgtc cactacggct acgacgataa gggccgcctg accggcgaac
      181 ggcagacggt ggagaacccg gagacggggg aactgctgtg gcagcatgag acaacacacg
      241 cctacaacga acaggggctg gcaaaccgcg tcacgccgga cagcctgccg ccggtggagt
      301 ggctgaccta cggcagcggt tatctggcgg gcatgaagct tggcgacacg ccgctgctgg
      361 agtacacgcg cgaccgcatg caccgggaga cggtgcgcag ctttggcagc atggcaggca
      421 gtaatgccgc atataaactg accagcacat acacccccgc aggccagtta cagagccagc
      481 acctgaacag cctggtgtat gaccgtgact acgggtggaa tgacaacggc gacctggtgc
      541 gcatcagcgg cccgcgacag acgcgggaat acggctacag cgccacgggc aggctggaga
      601 gcgtgcgtac cctcgcaccg gacctggaca tccgcatccc gtatgccacc gacccggcgg
      661 gaaaccgcct gccggacccg gagctgcacc cggacagtac cctcacggcg tggccggata
      721 accgcatcgc agaggatgcg cactatgttt accactatga tgaatacggc agactgacgg
      781 agaagacgga ccttatcccg gcgggtgtta tccggacgga cgacgagcgg acccaccact
      841 accactacga cagccagcac cgtctggtgt tctacacgcg gatacagcat ggcgagccac
      901 tggtcgagag ccgctacctc tacgacccgc tgggacggcg aatggcgaaa cgggtatggc
      961 ggcggaagcg tgacctgacg gggtggatgt cgctgtcgcg taaaccggag atgacgtggt
     1021 acgggtggga cggcgaccgg ctgacgacgg tacagaccga caccacgcgt atccagacgg
     1081 tatatcagcc ggggagcttc gcgccgctca tccgcatcga aacagacaac ggcgagcggg
     1141 agaaagcgca gcgccgcagc ctggcggaga agctgcagca ggaagggagc gaggacgggc
     1201 acggcgtggt atttccggct gaactggtgc ggctgctgga caggctggag gaagaaatcc
     1261 gggcagaccg cgtgagcagt gaaagccggg cgtggcttgc gcagtgcggg ctgacggtgg
     1321 agcaactggc cagacaggtg gagccggaat acacaccggc gcgaaaagtt catttttacc
     1381 actgcgacca ccggggcctg ccgctggcgc tcatcagcga agacggcaat acggcgtggc
     1441 gcggggagta tgatgaatgg ggcaaccagc ttaatgaaga gaacccgtat tacctgcacc
     1501 agccataccg tctgccgggg cagcagcatg atgaggaatc agggctgtac tataaccgga
     1561 accggtacta tgacccgcta caggggaggt atattacaca agaccccatt gggctggcgg
     1621 ggggatggaa cctgtataat tacccactga atccgataat aaggatggat cctttgggtt
     1681 tgtataattt atatcaatta ttatatgatg tttggcatga tgattcatat ggaacatcat
     1741 caattgatat tactggcagt ggagatctaa tatcattagg tggtcatgca ggacttggcg
     1801 ttgcgtttgc taaaaagaaa ggtgaaatgt tatctgatat ttgtatttat gctacagcat
     1861 gcggacatgc aggaattggt ggtgggataa atgcggctat cacatattct gagaccaagt
     1921 ctttacctac atcgggagtc agcaattcag taggtgtaac ggttggcggc ggagttgggg
     1981 ggcattttgc gtatacttat gtagtggatg ttgataatcc agaatcatcg acagaatctg
     2041 ttggtatcgg tgcaggtgtt gacgcttcag ttatgactct ggcttgtaga acgtggcaag
     2101 aatgctgggt caattaacag gaggaaatat gaaaaaatat atatttttag ttttattttt
     2161 attgtgtggt tgttctccag tggacataga gccagctaga caagaagtta atatctttcg
     2221 tggtttatat cagacaggga aatacagtga tatatataaa atcacttcaa gtgattttca
     2281 gactgccaca tctgaaaaaa atttatcgac ataatgaacg aagcaaaaga aaaagatctt
     2341 gggacttata aaaaat
//
LOCUS       DAZGSP010000255         2285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZGSP010000255 DAZGSP010000000
VERSION     DAZGSP010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.257"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(362..1187)
                     /locus_tag="ACHK74_005245"
                     /pseudo
     CDS             complement(362..1187)
                     /locus_tag="ACHK74_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1338..2111)
                     /locus_tag="ACHK74_005246"
     CDS             complement(1338..2111)
                     /locus_tag="ACHK74_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP0223880.1"
                     /translation="MLFSRMMMTGVLSLMLAGCGNLSHVTDEGTTEEVVWPSPEKVMF
                     NHDGTQKGSWPLPEKLRQIKAGMNKDQIYQLAGRPHFAEGMSGVREWDYLFNFTGPEG
                     DYTCQYKILFDRNMDARSFYWKPENCLPEAETAEYRESVPGDILFDFDSWTLTERGRD
                     ALSRVAEKFQAGHVVGVKIEGFTDHLGGQAYNRLLSEKRAESVRGWFIARGFRAERIH
                     ATGRGYDAPVVFCPDVEGEALKECLRPNRRVEITGQSVR"
     gene            complement(2136..2285)
                     /locus_tag="ACHK74_005247"
     CDS             complement(2136..2285)
                     /locus_tag="ACHK74_005247"
                     /inference="COORDINATES: protein motif:HMM:NF015831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFP0223881.1"
                     /translation="MGVGGYGGESAIAIGVSRISDNGKMIMKLNAGQNTRGNFSVGAG
                     VGWQW"
BASE COUNT          566 a          624 c          463 g          632 t
ORIGIN      
        1 aatcatcaca acaaagggaa aggtctgtag ataaagagtg ggatggactt cttacttcat
       61 atcacataat tgatattttt tatcctgcaa actccaacgg cattatgtat gacttagcct
      121 caggcaatta tcacaggcaa aaaaacaata tattgtaagc ttcatagaat ttggaacttt
      181 atataataaa taattctgag ttttttatca ccattatatt aacaaaatta atgtattcca
      241 gagatttcac attagtctcc aatttttcag aagaatctat ttacctggca ggaaaagtca
      301 tctggtgttt cgccggagtg aggggtacag gcaatagcgg acataaccat ttgttctttt
      361 tctaagcagc catctgataa tattttatcc tgaaggctgt cggggagata ttccccagac
      421 gagagtgacg acgctgacga ttgtagaaaa tctcaatgta ttcccgtatt actgagatgg
      481 cctcatcccg gcttttaaaa cgatagtggc tcagactttc atttttcagc gttccccaga
      541 agctttccat cggagcattg tcgtaacagt taactttacg cgacattgat attttcagac
      601 caaactgctc ctgtatggcc ctgtaatcgt atgcgcagta ctgtgaacca cgatcggagt
      661 ggtggattag cccggtaggt gagcgctggt tcctgagcgc cataaacagg gctttacccg
      721 tcaactattt cgtcatgcgc tctcccatgg cgtaaccgac aattttgctc gtataaacat
      781 ctttgacgcc agcgatatac agccatcctt ccggggggca acatacgtta tatctgcaac
      841 caagacctga ttaggtgctg taggcgtaaa gttctgattc aggagattcg gcgcaaccgg
      901 cagattgtga ccagagttcg tgttcgccct gaacttgcgt ttctgcttac agtgcagcct
      961 cagctcctta cgaagacgtg ccagtctgtc acgaccgacg ataattccgt tatctgccag
     1021 ctccgtctgg agcagctagg ttccatatgt ttcgcgtgtg cggatatgtg ccaccttaat
     1081 ctccagtttt agccgctcat cactttgttt tctgtctgag ggtcatgctg tactcagtcg
     1141 taataaccga tcctgtatac accaagtacc cgacacatcg cttcaataca taatcgcatc
     1201 tgaatcgttc accgattcgc ggcatttttc aaggtagttg tcgccattgt tcgactaact
     1261 gatcgacatt acagcctaga gcctagctgg gggctgtgca caaaaaagcc ccggacattc
     1321 cggggctaca ctgcatatta acgaacgctc tgtccggtta tttcaaccct gcggttgggg
     1381 cgcagacatt ccttcagtgc ttcgccctcc acatccgggc agaacaccac tggtgcatca
     1441 tatccacggc ccgttgcatg tatcctttca gccctgaatc cccgggcaat aaaccatccc
     1501 ctgactgatt ccgcccgttt ctctgacagg agccggttat acgcctgccc gccaagatgg
     1561 tccgtaaatc cttctatctt caccccgaca acatgacccg cctgaaactt ctctgccaca
     1621 cgggaaagtg catcacgccc cctctctgtc agagtccatg aatcaaaatc aaataatata
     1681 tcaccgggga ctgactccct gtattctgct gtctctgcct ccggcagaca gttttccggc
     1741 ttccagtaga aagagcgggc atccatgttc cggtcaaaca gtatcttgta ctggcaggta
     1801 taatccccct ccggtccggt aaagttgaac aggtaatccc attcccttac accagacatc
     1861 ccttcagcaa agtgtggtct ccccgccagc tgataaatct ggtccttatt catacccgcc
     1921 tttatctgac gtaacttttc tggcagtggc cacgaacctt tctgtgttcc gtcatgatta
     1981 aacatcacct tctccggaga aggccatacc acttcttccg tcgttccttc atccgtgaca
     2041 tgactcagat ttccacatcc tgcaagcatc agggacagca cacccgtcat catcattcga
     2101 ctaaataaca ttacatttct ccttgctttt ccctgttacc actgccagcc cacaccggca
     2161 ccgacggaga aattaccccg ggtgttctga cctgcattca gtttcattat catcttaccg
     2221 ttatcggaga ttcgggacac acctattgca atggcgcttt caccaccata tccaccgaca
     2281 cccag
//
LOCUS       DAZGSP010000256         2245 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZGSP010000256 DAZGSP010000000
VERSION     DAZGSP010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.258"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..183)
                     /locus_tag="ACHK74_005248"
     CDS             complement(<1..183)
                     /locus_tag="ACHK74_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFP0223882.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYQPCVNISAEPE
                     AYFRITPEDWLQAQMQG"
     gene            complement(194..892)
                     /locus_tag="ACHK74_005249"
     CDS             complement(194..892)
                     /locus_tag="ACHK74_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0223883.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSALTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(1102..>2245)
                     /gene="ompC"
                     /locus_tag="ACHK74_005250"
     CDS             complement(1102..>2245)
                     /gene="ompC"
                     /locus_tag="ACHK74_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP0223884.1"
                     /translation="TVALSAVAVAVMFAAGAQAAEVYNKDGNKLDLYGRATALHYFSD
                     DKGNDGDQTYARLGFKGETQINDQLTGFGQWEYQFSGNKTESEGSAGNKTRLAFAGLR
                     FADVGTIDYGRNYGIAYDVGSYTDVLPEFGGDGWTQTDNFMTARTSGVLTYRNTDFFG
                     LVDGLNFAAQYQGKNERDDLQKANGDGYGFSASYEFDGFGFVAAYTKSDRTDKQVQGL
                     NGTADVLDPASGKKVGEKAVDSGSVAKGKHAEFWGTGLKYDANNLYLAAVYSETQNMT
                     TFGDQGVADKAQNIEAVVQYQFDFGLRPSLAYLQSRGQDVMVGGVNHGDQDLVKYIDV
                     GATYYFNKNMSTYVDYKINLIDESEFTRKAGVATDNIVAVGMTYQF"
BASE COUNT          586 a          673 c          467 g          519 t
ORIGIN      
        1 cccctgcatc tgtgcctgca gccagtcttc cggtgtaata cgaaaatacg cctccggctc
       61 tgcggagata ttcacgcagg gctggtaccg ttcgccctcc ggggtgctta tcacgaagcc
      121 gcacgactcc gccggcgcac accgccgggc atgcgccaga atcgctgatt cagtctgtgt
      181 cataaagcgg gatttactgc gaaagtttat taatggaaag gaaaccgccg aaattagcca
      241 ccatgccgcg catctcacac ccgcgcatgc atttactgca tctgtccttc cggatatccg
      301 tggtggggtt gtcgaactca tccgccactg ccggaccgtt atacccgcat tcatcgcccc
      361 ggtaatccca catacaggtg ttcgccagca tgatgcgacc gggaaacagc gcaccgtccg
      421 tctccgtcgg tgtcgccagc acaaacgagg ccgtcatggc cgtcagcgct gacatctgct
      481 ccaccaccca ccggtccgtc agctcctgct ccgggtcggc ctccggattc cccgccacaa
      541 aattcaccgc atccagaaaa cgcgcataca cccggcggcg gaccaccgtg gcacccacca
      601 ggctctgcaa atcctccgcc atcccggtga caaggccgaa aagattggag accgtcagcg
      661 acgggcgggc actgctgccc tttccgttca tctcaaagcc gctgccctca atcgggtacg
      721 cctgatattc acgcccctgc caggtcaccg gctccccttt ttcattcagc tcattgcaga
      781 aaaaataccg ctcaccgccc tgcaccgtca ggtcgatttc ccagagcacc acccgcggtg
      841 actgctctga cttaaccgac tcgttcaggc tttcttcgtg aatatcctgc atatatccgc
      901 ccataaaaaa ggggcgcaga cgcgccccga acaataaaag tccaatttaa aataaaacca
      961 ataaaggtaa taacagaaag ttaaatcagc acatgccaga tggcaattgc tgatcgcagt
     1021 gaccaatgaa aactgtgttt tcattgatac aggccgcctg taagggcaga cggcccgtat
     1081 ttattaactt atcagcaata atcagaactg ataagtcata cccacagcaa cgatattatc
     1141 tgtcgcaaca ccggctttcc gggtaaattc actttcatca atcaggttaa ttttataatc
     1201 aacataggtg gacatattct tgttaaagta ataagtcgca ccgacatcaa tatatttaac
     1261 cagatcctga tcgccatggt tcacaccacc aaccataaca tcctgtccgc gggactgaag
     1321 gtaggcaagg gacgggcgca gaccaaaatc aaactgatac tgaacaacgg cttcgatatt
     1381 ctgagcttta tccgcaacac cttgatcacc aaaggtcgtc atattctggg tttcagaata
     1441 cactgctgcc agatacaggt tattggcatc atatttaaga ccagttcccc agaattcagc
     1501 atgtttacct tttgccacac tacctgagtc aactgctttt tctccaacct ttttacctga
     1561 tgcaggatca agaacatctg cagtaccatt caacccctga acctgcttat cagtacgatc
     1621 tgacttggta tacgcagcga caaaaccaaa accatcaaac tcatagctgg ccgagaaacc
     1681 atacccgtcg ccattagcct tctgaaggtc atcacgctca tttttgccct gatactgcgc
     1741 cgcaaaattc agaccatcaa ccagtccgaa gaagtctgta ttacggtatg tcaggacacc
     1801 ggaagttcgg gccgtcataa agttatcggt ctgcgtccag ccatcaccac caaactcagg
     1861 cagtacgtca gtatatgatc cgacatcgta agcaatacca tagttacgtc cgtaatcaat
     1921 ggtaccaaca tctgcaaatc tcagacctgc aaatgccaga cgggttttat ttcccgcgga
     1981 accttcagat tccgttttgt taccagagaa ctggtattcc cactgaccaa atccggtcag
     2041 ttgatcatta atctgcgttt cgcctttaaa gccgagacga gcataagtct ggtctccgtc
     2101 attaccttta tcatccgaga agtaatgcag agcggttgca cgcccgtaaa gatccagttt
     2161 attaccatct ttattataaa cttctgctgc ctgcgccccc gcagcaaaca tcactgccac
     2221 tgctacagca gaaagtgcca ctgtc
//
LOCUS       DAZGSP010000257         2225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZGSP010000257 DAZGSP010000000
VERSION     DAZGSP010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.259"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(110..1108)
                     /locus_tag="ACHK74_005251"
     CDS             complement(110..1108)
                     /locus_tag="ACHK74_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFP0223885.1"
                     /translation="MKNPELKNLTDYSPADKPWDEHRSFSDDVGGIYLRAAGFEHYGE
                     RVGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPK
                     IVTEHPGARWLFLTLTVRNCSIDALGETLTSMNAGFKRLKERKEFRHVLGWVRTTEVT
                     RGKDGSVHPHFHTLLMVPPSMLSGREYVKHARWVELWRDCLRVAYDPNVDIRVVKSRK
                     PKDDESLPCVVAEQLRGAVVETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGAL
                     KNVLQLERETNEDLIMADGMAEGTDDGSRLAFSWVPIVKKYQRDRAKDKRQLSGED"
BASE COUNT          547 a          647 c          540 g          491 t
ORIGIN      
        1 aaaaaaaacg ggcaaaacct tgcccgtttc tgaaaatcac gttagaacgc ttctgagcgc
       61 gtttctgaat attctcccag tgcatgaata gttcgcgcct gaaacgcgct caatcctcgc
      121 cacttagctg acgtttatct ttcgcccgat cgcgctggta ttttttgaca atcggcaccc
      181 acgaaaacgc cagacgggaa ccgtcatctg taccctctgc catgccatcc gccatgatta
      241 aatcctcgtt cgtttcccgc tccagttgca gaacgttctt aagcgctccc cccgtagcaa
      301 caaaacgacg cttgtgtacc tgccgtgtta attcgaggaa ccattcagga tcagccagca
      361 tatcagcagg ctttgtggaa tatttcagag tttcaaccac cgcgccgcgc agctgttccg
      421 caacaacaca aggtaaagat tcatcgtctt ttggtttccg tgacttcacc actctgatat
      481 ccacattcgg gtcatacgcc acccgcagac aatcacgcca cagttccacc caacgggcat
      541 gtttcacata ctcacggccg gacaacatcg aagggggaac catcagcaga gtgtgaaaat
      601 ggggatgtac gctaccatcc ttgccgcgcg ttacttccgt ggtacgaacc catcccaaca
      661 catggcgaaa ttccttacgc tctttcaacc gcttaaaccc cgcattcatc gaggtaagcg
      721 tctcaccaag cgcgtcaatg gaacagttgc gcactgtcag tgtgagaaac aaccagcgcg
      781 cgccgggatg ttccgtcaca atctttggca gggactgata gaaacgggct tgccacatca
      841 tggaacgcct ccactgacag atcggacagt gccggacgcg acagaaccgt gcatcacgca
      901 accgcaggcg cgtttctccg gtctctgtgt ttgttgacca cccaaaccgc aaaataccac
      961 tacaagcccc gacgcgttcg ccgtagtgct caaatccggc agcacgcaga taaataccgc
     1021 ccacatcatc ggaaaagctt cggtgttcat cccacggttt atctgctgga gagtaatccg
     1081 tcagattctt aagttcagga tttttcatgt ctgccgtccc cttgcgagcg acagacgaaa
     1141 ttgcgtgtta tcattcctgt atcacctgta ccaattgtcg tcaaatcagt tcatgttcta
     1201 ggtggtcgcg cccctggagt ggtggaagac tcgcaggggc taatttataa ccactcccga
     1261 caaaatactc tcaaacctat ttacacgcaa atccgcgctc aggccagata tggcaaggga
     1321 tagaggcgat ccaaaaaatc gcatttatcc cttaagtata aatacaagga aacgcgcgcg
     1381 aagatagcag caaaatcgcc tgttttttgt tcgattcccc gagggcaaaa acaacaaccg
     1441 cgaaagcgtt ttcccgacaa aacacacaca ggcttttcca ccgattttgt gaacagcaaa
     1501 aaccgtcttg caggtggttg gtctcactcc gtgacaccaa ccaccatccc cagtgttttt
     1561 cccgtgccgt cgtcaatcac aaaatcggaa ccccggaggg gcccccgcga ttttctgttt
     1621 gacgagttcg tgaacggtga gcgggacgcg gaattttttc gcgtgccgtg aggcgttggc
     1681 ggggtgtcgg ggtgcagccc tgaccaggtg gggaatgtct gagtgcgcgt gcgcggtccg
     1741 acattcccac atcctgtccc gaaaattccc gccgtttctc ggttttttga gcgtcgcaga
     1801 ccgcacgaca aacgaaacga agtgtagttt gtatgagtgc gcacttcgtg agattccccg
     1861 actgccagtt tgtcgatcgc gcggaacagc tgtggcacga aaacggcaga atgatttgct
     1921 gtccggttgc cagaactgcg cgcttacgtg gagggcggcg cggcgcaaaa actgccgaca
     1981 ttgcgccagt gttccggtga cacaaacccg cagaaatcgg gacaggatgt gtaatcgtat
     2041 aaccgcgcac gcacgccgat acgattacct cctggtcagg gctgcacccc gacaccctcg
     2101 acaacctgcc cgggacacgt taaaacgaca tctgcgcgtt tctggtgagc gaaaagctgt
     2161 cagccgtgga ttgccgtagc agagacccgg aaacgcgctt aaaacgcttc tgagcgcaga
     2221 gaaac
//
LOCUS       DAZGSP010000258         2156 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZGSP010000258 DAZGSP010000000
VERSION     DAZGSP010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.260"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..992)
                     /gene="subA"
                     /locus_tag="ACHK74_005252"
     CDS             complement(<1..992)
                     /gene="subA"
                     /locus_tag="ACHK74_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFP0223886.1"
                     /translation="MLKILWPHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKESEALYIHGTAMASLIASRHG
                     VYGVYPHALISSRRVIPDGVQDSWIRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWTELLSRMGRNNERLIVAAVGNDGADIRKLSAQQRIWPAAYHPVSSMNKKQDPVIRV
                     AALAQYRKGETPVLHGGGITGSRFGNGWVDIAAPGQNITFLRPDGKTGTGSGTSEATA
                     IVSGVLAAMVSCNPRATATELKRTLLESADKYPSLADKVTEGRVLNAEKAISMF"
BASE COUNT          608 a          495 c          460 g          593 t
ORIGIN      
        1 cagaacatgc taattgcttt ttctgcatta agaaccctcc cttccgtcac cttatctgca
       61 agtgaggggt atttgtctgc agactccagc agtgtacgct tcagttctgt cgctgttgca
      121 cggggattac atgagaccat cgctgccaga acgccggaaa ctatagctgt tgcttccgag
      181 gttccactac ctgtccccgt ttttccatct ggtctgagga atgtaatatt ctgtcccggt
      241 gcagcaatat caacccagcc gtttccgaac cgacttccgg tgatccctcc accatgcaat
      301 acaggtgttt ctcctttccg gtactgtgcc agagctgcca ctcttatcac aggatcctgc
      361 tttttattca tactgctgac cggatggtaa gccgcaggcc atattctctg ctgagcactc
      421 agttttcgta tatctgcgcc atcattaccc actgccgcaa caattaatcg ctcattattc
      481 cggcccatac gggaaagcag ttcggtccag accgatgcgg aagatatccc cttctggcct
      541 cccgatatat taatgatttt ctcttctcct ggtgcaagaa aaacatttga cataatgctt
      601 tcagttgctc taatccatga gtcctgtaca ccgtcaggaa taacccttct gctggatatc
      661 agtgcatgag gataaacacc ataaacccca tgacgtgagg caatgagtga agccatggca
      721 gtaccatgaa tatataaagc ttcagactct tttaccggga atggcgaacc atcctgagta
      781 aaactgaatt tggcaaattc gctgtcgctg aggcctccta caaatgccac tccggaatca
      841 acaacagata cgacaacagg ggtattcttg tctgtgagcg acatggttgt ttctgtcagg
      901 ccaattgcat caaaatacca gggtttttct gccctgacag acacagatat aaaaagcagg
      961 aacagaatat gcggccataa aatcttaagc acaatatcct ccagtcagta caaataaaca
     1021 gtattgcagg tcaatatctt acaggaacat cacatctgaa atatcctgtt ttcgacaaaa
     1081 gataaacaat cacaaccaat aaagcgagtg aagcccgtct caaaaacatt aatttttata
     1141 tttatgtatc caatgaatta acgctcacct tcacggtaat atgcatctgg atttctgcaa
     1201 caggtcagcg ataagatgct ctgcctcctc cttcagagca gaagtaaata cagccatgat
     1261 ggataaaact taaccagaaa aaataagagg aggcaagtaa gttgcctggc atgtttatga
     1321 caataaaaag ttccgtcagg catcatggcg aacaggatat cacagcgccg ttttaccggg
     1381 gcaatcagtg aatggtggtg tggttcattg gtaagccccg gatatcaggt cactgagtat
     1441 attcatgaat gaccgttaaa ccggggatgc gagctgctgc ccgggaaagt cgaactgacc
     1501 ggtcaataga tatcaatacg gggcagtcag tccacttaca ggtatctgac aaggacacct
     1561 tctgagcgtg caatgtggac gggtaataca acgatggtag caggctggtc agcaatcagc
     1621 agtatatgcc catatttcct gaggccggat atctgtttcc tgagcttgtt ttcgacgttt
     1681 gctaatattt tgtcgaacta attatcagaa cgattaatga taacagtgtg gcagggatct
     1741 ttaccgatat caacgtaaat aatgacctta acaggtgcgt aattgctgga ttttgccatg
     1801 ctgttaccct gttgatacgg gttaattaaa taactttggg gaagcaggta ccggcgttca
     1861 ctaccgctct ccctgataat gtatcctgca tgcctgataa cttttgccgg gctaatgtaa
     1921 aataaccctg aaatatatta agttgattaa cagactggtg atggaaaaaa caggcattat
     1981 ttattatata aaaatccgga tttgatgcgg cttgcgatat attatttttt gcctgtgatg
     2041 ggagcctgag gttgaattat atataatgca actggaggtt gcgggataaa gtatatcaat
     2101 tatgtgacat gaagcagaaa gttcctcccc ctcttatcca cagacctttc cctttg
//
LOCUS       DAZGSP010000259         2141 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZGSP010000259 DAZGSP010000000
VERSION     DAZGSP010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.261"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..587)
                     /locus_tag="ACHK74_005253"
     CDS             complement(<1..587)
                     /locus_tag="ACHK74_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0223887.1"
                     /translation="MWVTAKECVGLPDLPMMEHNIRHRLTKAAAGNAAMLRKRKGTKA
                     IEFNIDCLPSGAQQVLRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRQVADARIAIVSEVMKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAA"
     gene            complement(598..864)
                     /locus_tag="ACHK74_005254"
     CDS             complement(598..864)
                     /locus_tag="ACHK74_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0223888.1"
                     /translation="MRVSRQASNNLFVREGCDWSAKAVYAALENKGTDLPELERELGL
                     KSGSMRNVFYRQCRKYEAAIAEKIGVEPAVIWPSRYQVDAREVA"
     gene            1021..1632
                     /locus_tag="ACHK74_005255"
     CDS             1021..1632
                     /locus_tag="ACHK74_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0223889.1"
                     /translation="MDKAEKTVSSAERFIDMEGINRFSERLAMAMGDMTNVELATLCG
                     LSEATVRNYRKGKNFPPLDKLALIAKACNCSLEWLATGKNEDECGKIENNCDSGSGID
                     QALSSLSPQEKQLILTFIRREGTNNLVRLAAMNTSTISQDAVESIIDALPLRPVLKNA
                     IKIGMAGSEETDKEILRVIEKYNSSNNATQESGAVTVKKNVVG"
BASE COUNT          591 a          468 c          466 g          616 t
ORIGIN      
        1 gcggcgttct gcgccttgtt aaaggctgca atccagcgtt tcagcgtgat ttcactcaac
       61 gcacggctgt ttcccttttt ggcgttcgcc gtctcaacca ttgccacaat gcgctcgtcc
      121 agctgcgaac gtgccaggtt gtcaacgata aaccggatag ctttcgcaca gctgaaaccg
      181 ggttgttgcg cgactttcat cacttcgctg acgatcgcga ttcgtgcatc agccacctgg
      241 cgctggtttt cagtcagggc attaaggcgt tcaaccatca gttgtggtga tccgcgatat
      301 gcctccaccg catcaaccac ggcaggcgag cgtctggcct ttgtcaccac cggagccggt
      361 gattcatcgg ctttttgcgt catcagttga agggcataac gttcacgcaa tacttgttgc
      421 gcgccagaag gcaaacaatc gatattaaat tcgattgctt ttgtcccctt acgtttgcgt
      481 aacatggctg catttcccgc agctgctttt gtgagtcgat ggcgaatgtt atgttccatc
      541 attggtaaat caggaagacc aacacattct tttgctgtta cccacataca tcccccatca
      601 agccacttct ctggcgtcca cctggtatct gctgggccag attacagcag gttcaacccc
      661 tattttttcg gcgatggcgg cttcgtattt acggcactgg cggtaaaaca cattgcgcat
      721 tgatccgctt tttaatccta actccctttc cagttcaggg aggtctgtgc ctttgttttc
      781 aagggctgca tataccgctt tagcagacca gtcacacccc tctcgaacaa acaaattatt
      841 cgatgcttgt ctggataccc tcattgtgtg atcctctcat gtttacctga accgataatc
      901 aaaaccggtt atcgatttag gtaaatcata atcgcaaaat aattcgatat caacggtttt
      961 ttgcgagcat atcgaaatta aatgcgatat cgataatttc tttgttttat agggggttat
     1021 atggataagg cagaaaaaac cgtatccagt gcggaacggt ttattgatat ggaaggaata
     1081 aaccgatttt cagaacgctt agctatggct atgggagaca tgacaaacgt cgaactggcg
     1141 accctttgtg ggctatctga agccacagta agaaattaca ggaaggggaa aaacttccct
     1201 cctttggata aactggcctt aatagctaaa gcctgtaatt gttcactcga atggttggcc
     1261 acaggcaaga acgaagatga atgcggaaaa atcgaaaata attgcgattc aggttcgggt
     1321 attgatcagg cgcttagctc tctaagcccc caagagaagc aattaatcct tacattcatc
     1381 aggcgagaag gtactaacaa ccttgtcagg ctggcagcaa tgaatacatc tacaatcagc
     1441 caggatgcgg tagaaagcat cattgatgcc ttgccattac gtcccgtact aaaaaatgca
     1501 ataaagatag gaatggcggg aagtgaagaa acagacaaag agattttgcg cgttattgag
     1561 aagtacaact cgagcaataa cgccacccag gaatcagggg cagtgacagt aaagaaaaat
     1621 gttgttggtt aagcgccatc gctatcatga ggtggtgctg taaatacata tagagatcat
     1681 atgactcatc ccaagataat cacgctattg aatagtaatt catgatgatt atcatactga
     1741 ttatagttag atcgattatg tgagcatttt ggcagtttga agatgttttg aagaagattt
     1801 taagggtaaa ttagcagtat caaatgtgtc gccgtttttt gatcttttga ctatttgttg
     1861 agcagtcggt atcaaaaaga gttttccgtc cgaactatac atcacctgtt ttatttcagc
     1921 atcttacagg tgatttcacc tcctttcact aaaacccttg tcggtatcaa atgattcacc
     1981 tggttacaat ctgtataaat atccattaaa ccctgttcgg tttatagatt ctttgggttt
     2041 aaaattccat gttaacggtg atccaagtga ttttaatcag gctgttgagt atttaaaaca
     2101 agattcacaa atgaaagaaa ctatagattt tttatcaagc t
//
LOCUS       DAZGSP010000260         2140 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZGSP010000260 DAZGSP010000000
VERSION     DAZGSP010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.262"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2111
                     /locus_tag="ACHK74_005256"
     CDS             <1..2111
                     /locus_tag="ACHK74_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP0223890.1"
                     /translation="TLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSHTSEGHRV
                     SVHYGYDDKGRLTGERQTVENPETGELLWQHETKHAYNEQGLANRVTPDSLPPVEWLT
                     YGSGYLAGMKLGGTPLVEYTRDRLHRETVRSFGSRAGSNAAYELTSTYTPAGQLQSQH
                     LNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPGLDIRIPYATDP
                     AGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDRIPTGVIRTDDER
                     THHYHYDSQHRLVFHTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRK
                     PEETWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQCRSLAEKLQ
                     QEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSDESRAWLAQCGLTVEQLARQVEPEY
                     TPARKVHFYHCDHRGLPLALISEDGNTVWSAEYDEWGNQLNEENPYYLYQPYRLPGQQ
                     YDEESGLDYNRHRYNDPLQGRYITQDPIGLAGGWSLYAYPLNPVNGIDPLGLSPADVA
                     LIRRKDQLNHQRAWDILSDTYEDMKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKG
                     LGYEKEIRDYGLNLFGMYGRKVKLSHSEMIEDNKKDLAVNDHGLTCPSTTDCSDRCSD
                     YINPEHKKTIKALQDAGYLK"
BASE COUNT          552 a          543 c          674 g          371 t
ORIGIN      
        1 tcaccctctg gcactacgat gcgtcggacc gcatcacgca ccggacggtg aacggcgacc
       61 cggcagagca gtggcagtat gatgagcacg ggtggttaac caccctcagc cataccagtg
      121 aaggccaccg cgtgtcggtc cactacgggt acgacgataa gggccgcctg acgggcgaac
      181 ggcagacggt ggagaacccg gagacggggg aactgctgtg gcagcatgag acgaaacacg
      241 cctacaacga acaggggctg gcaaaccgcg tcacgccgga cagcctgccg ccggtggagt
      301 ggctgaccta cggcagcggt tacctggcgg gcatgaagct gggcgggacg ccgctggtcg
      361 agtatacgcg ggacaggctg caccgtgaga cggtgcgcag cttcggcagc agggcaggca
      421 gtaatgccgc atatgaactg accagcacat acacccccgc aggccagtta cagagccagc
      481 acctgaacag cctggtgtat gaccgtgact acgggtggaa tgacaacggc gacctggtgc
      541 gcatcagcgg cccgcgacag acgcgggaat acggctacag tgccacgggc aggctggaga
      601 gcgtgcgcac cctcgcaccg ggcctggaca tccgcatccc gtatgccacc gacccggcgg
      661 gaaaccgcct gccggacccg gagctacacc cggacagcac gctcacggcg tggccggata
      721 accgcatcgc agaggatgcg cactatgttt accactatga tgaatacggc agactgacgg
      781 agaagacgga ccgcatcccg acgggtgtga tacggacgga cgacgagcgg acccaccact
      841 accactacga cagccagcac cgcctggtgt tccacacgcg gatacagcat ggcgagccac
      901 tggtcgagag ccgctacctc tacgacccgc tgggacggcg aatggcgaaa cgggtatggc
      961 ggcgggagcg tgacctgacg gggtggatgt cgctgtcgcg taaaccggag gagacatggt
     1021 acggctggga cggcgacagg ctgacgacgg tacagaccga caccacgcgt atccagacgg
     1081 tataccagcc ggggagcttc gcgccgctca tccgcatcga aacagacaac ggtgaacggg
     1141 agaaagcgca gtgccgcagc ctggcggaga agttacagca ggaagggagc gaagacggtc
     1201 acggtgtggt gttcccggct gaactggtgc ggctgctgga cagactggag gaagaaatcc
     1261 gggcagaccg cgtgagtgac gaaagccggg cgtggcttgc gcagtgcggg ctgacggtgg
     1321 agcaactggc cagacaggtg gagccggaat acacaccggc gcgaaaagtt catttttacc
     1381 actgcgacca ccggggcctg ccgctggcgc tcatcagcga agacggcaat actgtgtgga
     1441 gtgcagaata cgatgaatgg ggcaaccagc tgaatgaaga gaacccgtat tacctgtatc
     1501 agccataccg tctgccaggg cagcagtacg atgaggagtc ggggctggat tataaccgtc
     1561 accggtacaa cgatccgttg caggggcggt atatcaccca ggacccgatt gggctggcgg
     1621 ggggatggag tctgtatgcg tatccgctga atccggtgaa tggtattgat ccattagggt
     1681 taagtcccgc agatgtagcg ctaataagaa gaaaagatca actaaaccat caaagagcat
     1741 gggatatatt atctgatact tatgaagata tgaagagatt aaatttaggt gggactgatc
     1801 aatttttcca ttgtatggca ttttgtcgag tgtctaaatt aaatgacgct ggtgttagcc
     1861 gatcggcgaa agggctgggt tatgaaaaag agattagaga ttacgggtta aatctgttcg
     1921 gtatgtacgg cagaaaagta aagctatccc attctgaaat gattgaagat aataaaaaag
     1981 acttggctgt aaatgaccat gggttgacat gtccatcaac aacagattgc tcagatagat
     2041 gtagtgatta tattaatcca gagcataaaa aaacgataaa ggctttacaa gatgctggct
     2101 atctcaagta atctatcaaa gatgataata tttatttttg
//
LOCUS       DAZGSP010000261         2121 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZGSP010000261 DAZGSP010000000
VERSION     DAZGSP010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.263"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..234)
                     /locus_tag="ACHK74_005257"
     CDS             complement(<1..234)
                     /locus_tag="ACHK74_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP0223891.1"
                     /translation="MHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVVAG
                     LVLWYQWRCLSSRRDIPENAGDDGLPPEYFQGHV"
     gene            complement(237..890)
                     /gene="tssL"
                     /locus_tag="ACHK74_005258"
     CDS             complement(237..890)
                     /gene="tssL"
                     /locus_tag="ACHK74_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFP0223892.1"
                     /translation="MNKPVISRAEQIFYPGWLMVCQLRSGQPVEDGKALYRRACQLVK
                     QAREELAEAGFSQENSEIMLYAFCALLDESVLNREKTDDGWHTWQQDPLQAHFFGTLN
                     AGEELWERIREQLKLPAPDVAVLTCLCRTLQLGFTGQYRSQDDERREDVIRALTARVP
                     AFTFAQDAPVVVRAPGYRAGRTMYWLSWVAGIMTLAGLWCVLSSVLADQVARLTGQG"
     gene            complement(887..>2121)
                     /gene="tssK"
                     /locus_tag="ACHK74_005259"
     CDS             complement(887..>2121)
                     /gene="tssK"
                     /locus_tag="ACHK74_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP0223893.1"
                     /translation="MGLAHPWGVVAAEFDDSLLPLSRLNATRLIVRFPDGTLIDTERA
                     DNLPPVCDLSTVSDRSLVDIVLALPLLNANGGNLDDGRESERPRRWKSERVNVQELAG
                     HEQSEVAVLRHNLTLRMAHQENAAWLTCPVTRLVRDAQGQWCRDPRFIPPLLTLSASP
                     SLMTELAELLHHLQARRQRLMSMRRENNARLADFAVADVSLFWLLNALNSAEPVLKEL
                     LDMPYRHPELLYRELARLAGSLLTFSLEHNVDAVPAYRHETPENVFPPLLSLLNQLLE
                     ASLPSRVVFIELKQNGVMWEGALHDARLREGADFWLSVRSSMPGHELQTKFPQLCKAG
                     SPDDVSEVVNVALSGVIIRPVTHVPAAIPLRLENQYFALDLSTDAARAMLDAGRCTFY
                     TPASLGDVKLELFAVLRT"
BASE COUNT          537 a          659 c          535 g          390 t
ORIGIN      
        1 cacatgcccc tgaaaatact caggcggcag accgtcatcg ccggcattct ccgggatatc
       61 acgacgggaa gacagacaac gccactgata ccacagcaca agccccgcca ccacgactga
      121 aaccagggtc agtattatct ggtttactgc agacagtggc aggaatcccg aaatcagcca
      181 cagtgtcagg aacagactca gtattgtcag ccagagacgg gaacgtacgt gcatggttat
      241 ccctgcccgg taagccgtgc cacctggtct gcgagcacag aagaaagcac acaccagaga
      301 ccagccagcg tcataatacc cgccacccat gacaaccagt acatcgttct gccagcacgg
      361 taccccggtg cacgcaccac caccggcgca tcctgtgcga aagtaaatgc aggaacacgc
      421 gctgtcagtg cgcgtattac atcttcgcga cgctcatcat cctgcgaccg gtactgaccg
      481 gtaaaaccga gctgaagcgt acggcagagg catgtcagta ccgccacatc cggcgctggt
      541 aatttcagtt gctcacgaat acgctcccag agctcttcac cggcattgag cgtaccaaaa
      601 aaatgagcct gcagcgggtc ctgctgccag gtgtgccagc catcgtctgt tttttcacgg
      661 ttcagcacac tctcgtccag cagggcgcag aaggcataca acatgatttc actgttttcc
      721 tgactgaatc ctgcttcggc cagctcttca cgcgcctgtt tcaccagctg acaggcacgg
      781 cgatacaggg ccttaccatc ctcaaccggt tgtccgctgc gcagctggca gaccatcagc
      841 cagccgggat aaaaaatctg ttcagcccgg gagataacag gtttattcat gtccgcagta
      901 ccgcaaaaag ttccagtttc acatccccca gcgatgccgg ggtgtagaaa gtacaacgcc
      961 ccgcgtccag catcgcccgg gccgcatccg tgcttaaatc cagcgcgaaa tactggtttt
     1021 ccagccggag cggaatggcc gccggtacat gcgtaaccgg acggataatg accccgctca
     1081 gcgccacatt caccacctca gacacatcat cggggctccc ggctttgcat aactgcggaa
     1141 acttcgtctg cagttcatgc ccgggcatgg aagagcgcac tgaaagccag aaatccgccc
     1201 cttcgcgcag acgcgcatcg tgtaatgccc cctcccacat cacaccattt tgtttcagtt
     1261 caatgaaaac cacacgggaa ggcaggcttg cctccagcag ctggttcagc aaagaaagca
     1321 aaggtgggaa aacattttca ggtgtttcat gacggtatgc tggaaccgca tccacattat
     1381 gctcaagcga gaatgtgagc agggagcctg ccagccgggc aagctccctg tacaacagtt
     1441 ccgggtggcg gtaaggcatg tcaagcaact ctttcagaac aggctccgca ctgttcaggg
     1501 cattcagcaa ccagaacaac gacacgtccg ccactgcaaa atccgcaaga cgggcattgt
     1561 tctcccgacg catggacatc agtcgctggc gacgcgcctg caggtgatga agcaactccg
     1621 caagctcagt catcagcgaa ggactggcag acagcgtcag cagaggggga ataaaacgcg
     1681 ggtccctgca ccactgtccc tgtgcatcac gaacaagacg ggtaacggga caggtcagcc
     1741 aggcagcatt ttcctgatga gccatgcgca gagtcaggtt atgccgcaat accgccactt
     1801 cgctttgctc atgcccggcc agctcctgca cattcaccct ttctgatttc cagcgacgcg
     1861 gacgctcgct ctcacggcca tcatcaaggt tgccaccgtt agcattcagt aacggtagcg
     1921 ccagcacaat atccaccagg gaacgatcag aaaccgtcga taaatcacag accggaggca
     1981 gattatccgc acgttccgta tcaataagcg ttccgtccgg gaaacgaaca atcagacggg
     2041 tggcattcaa ccgtgaaaga ggtaacagag agtcatcaaa ctctgcggcc acaacgcccc
     2101 agggatgtgc cagccccatt c
//
LOCUS       DAZGSP010000262         2121 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZGSP010000262 DAZGSP010000000
VERSION     DAZGSP010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.264"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..234)
                     /locus_tag="ACHK74_005260"
     CDS             complement(<1..234)
                     /locus_tag="ACHK74_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP0223894.1"
                     /translation="MHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVAAG
                     LVLWYQWRCLSSRRDIPENAGDDGLPPEYFQGHV"
     gene            complement(237..890)
                     /gene="tssL"
                     /locus_tag="ACHK74_005261"
     CDS             complement(237..890)
                     /gene="tssL"
                     /locus_tag="ACHK74_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFP0223895.1"
                     /translation="MNKPVISRAEQIFYPGWLMVCQLRSGQPVEDGKALYRRACQLVT
                     QAREELAEAEFSQESSEIMLYAFCALLDESVLNREKTDDGWHTWQQDPLQAHFFGTLN
                     AGEELWERIREQLKLPAPDVAVLTCLCRTLQLGFTGQYRSQDDERREDVIRALTARVP
                     AFTFAQDAPVVVRAPGYRVGRTMYWLSWVAGIVTLAGLWCVLSSVLADQVARLTGQG"
     gene            complement(887..>2121)
                     /gene="tssK"
                     /locus_tag="ACHK74_005262"
     CDS             complement(887..>2121)
                     /gene="tssK"
                     /locus_tag="ACHK74_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP0223896.1"
                     /translation="MGLAHPWGVVAAEFDDSLLPLSRLNATRLIVRFPDGTLIDTERA
                     DNLPPVCDLSTVSERSLVDIVLALPLLNANGGNLDDGSESERPRRWKSERVNVQELAG
                     HEQSEVAVLRHNLTLRMAHQENAAWLTCPVTRLVRDAQGQWCRDPRFIPPLLTLSASP
                     SLMTELAELLHHLQARRQRLMSMRRENNARLADFAVADVSLFWLLNALNSAEPVLKEL
                     LDMPYRHPELLYRELARLAGSLLTFSLEHNVDAVPAYRHETPENVFPPLLSLLNQLLE
                     ASLPSRVVFIELKQNGVMWEGALHDARLREGADFWLSVRSSIPGHELQTKFPQLCKAG
                     SPDDVSEVVNVALSGVIIRPVTHVPAAIPLRLENQYFALDLSTDAARAMLDAGRCTFY
                     TPASLGDVKLELFAVLRT"
BASE COUNT          541 a          663 c          528 g          389 t
ORIGIN      
        1 cacatgcccc tgaaaatact caggcggcag accgtcatcg ccggcattct ccgggatatc
       61 acgacgggaa gacagacaac gccactgata ccacagcaca agccccgccg ccacaactga
      121 aaccagggtc agtattatct ggtttactgc agacagtggc aggaatcccg aaatcagcca
      181 cagtgtcagg aacagactca gtattgtcag ccagagacgg gaacgtacgt gcatggttat
      241 ccctgcccgg taagccgtgc cacctggtct gcgagcacag aagaaagcac acaccagaga
      301 ccagccagcg tcacaatacc cgccacccat gacaaccagt acatcgttct gccaacacgg
      361 taacccggtg cacgcaccac caccggcgca tcctgtgcga aagtaaatgc aggaacacgc
      421 gctgtcagtg cgcgaattac atcttcgcga cgctcatcat cctgcgaccg gtactgaccg
      481 gtaaaaccga gctgaagcgt acggcagagg catgtcagta ccgccacatc cggcgcaggt
      541 aatttcagtt gctcacgaat acgctcccag agctcttctc cggcattgag cgtaccaaaa
      601 aaatgagcct gcagcgggtc ctgctgccag gtgtgccagc catcgtctgt tttttcacgg
      661 ttcagcacac tctcgtccag cagggcgcag aaggcataca acatgatttc actgctttcc
      721 tgactgaatt ctgcttcagc cagctcttca cgcgcctgcg tcaccagctg acaggcacgg
      781 cgatacaggg ccttaccatc ctcaaccggt tgtccgctgc gcagctggca gaccatcagc
      841 cagccgggat aaaaaatctg ttcagcccgg gagataacag gtttattcat gtccgcagta
      901 ccgcaaaaag ttccagtttc acatccccca gcgatgccgg ggtgtagaaa gtacaacgcc
      961 ccgcgtccag catcgcccgg gctgcatccg tacttaaatc cagcgcgaaa tactggtttt
     1021 ccagccggag cggaatggcc gccggtacat gcgtaaccgg acggataatg accccgctca
     1081 gcgccacatt caccacctca gacacatcat cggggctccc ggctttgcat aactgcggaa
     1141 acttcgtctg cagttcatgc ccgggtatgg aagagcgcac tgaaagccag aaatccgccc
     1201 cttcgcgcaa acgcgcatca tgtaatgccc cctcccacat cacaccattt tgtttcagtt
     1261 caatgaaaac cacacgggaa ggcaggcttg cctccagcag ctggttcagc aaagaaagca
     1321 agggtggaaa cacattttca ggtgtttcat gacggtatgc cggaaccgca tccacattat
     1381 gctcaagcga gaatgtgagc agggagcctg ccagccgggc aagctccctg tacaatagtt
     1441 ccgggtggcg gtaaggcatg tcaagcaact ctttcagaac aggctccgca ctgttcaggg
     1501 cattcagcaa ccagaacaac gacacgtccg ccactgcaaa atccgcaaga cgggcattgt
     1561 tctcccgacg catggacatc agtcgctggc gacgcgcctg caggtgatga agcaactccg
     1621 caagctcagt catcagcgaa ggactggcag acagcgtcag cagaggggga ataaaacgcg
     1681 ggtccctgca ccactgtccc tgtgcatctc gaacaagacg ggtaacagga caggtcagcc
     1741 aggcagcatt ttcctgatga gccatgcgca gagtcaggtt atgccgcaat accgccactt
     1801 cgctttgctc atgcccggcc agctcctgca cattcaccct ttctgatttc cagcgacgcg
     1861 gacgctcgct ctcactgcca tcatcaaggt tgccaccgtt agcattcagt aacggtagcg
     1921 ccagcacaat atccaccagg gaacgctcag aaaccgtcga taaatcacag accggaggca
     1981 gattatccgc acgttccgta tcaataagcg ttccgtccgg gaaacgaaca atcagacggg
     2041 tggcattcaa ccgtgaaagc ggtaacagag agtcatcaaa ctctgcagcc accacgcccc
     2101 agggatgcgc cagccccatt c
//
LOCUS       DAZGSP010000263         2105 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZGSP010000263 DAZGSP010000000
VERSION     DAZGSP010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.265"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2101
                     /locus_tag="ACHK74_005263"
     CDS             <1..2101
                     /locus_tag="ACHK74_005263"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223897.1"
                     /translation="PVSVMAKAQKGSVINSITFTDSLTDETDDKHDFTNTGDLKGSTP
                     SIMTFPYGESDDRYTTNYVGNVQSSKTKFINLATGLTNDTTPTILISLDKELNNNQHI
                     EITRYKVDVDNDNILYEVVDEIIPSEHVDIKGKNIIVKDQLEHTYSQYYKYEFVIKDN
                     VDGKESVTSEKEFYFLLDTDVEAFDIHKIDKTKDNILFSGTGENNTQVMIKYKTERGE
                     EKNIKVVVDDTGKYEINLNGWDIKDADGAEVKIVDSAGNVKSGNLYNIARLYVDMNTN
                     KAITLDSAYNIIGSQKEGTDPAKALIMSDDNDWVYIGGGISPHIGDQSPGSDNNIDMA
                     GGDDILSSVGAVLDGANINMGDGDDKLYTQDGFASSGTRNIIMGDGNDVISVDNSFGG
                     KNTISLGEGNNLFIVGNYVNSIAENDITAGSGDDRIEIGTNLDGKNKIDLGDGDNTIQ
                     VGGYITNSHTITGNSGDDIIYVATNIDGSGSFNLGDGNNNFIVGGYIQGKNTIEMGSG
                     DDTVSVSTRIADNVKIQLNAGDDSVYAGGLLNKAIVDLGDGDDVVTLSGISDNGKRNN
                     MEELVSTNAMLTGGEGNDTLKINGSFKLLNMKNISGFETIDLGESSENHLDVGIKSDM
                     LDISSSSGVKIFTIMGGAGNTVDLGKANITSHNSVEQGNYADSWYKGDTVDGYTTYTP
                     VGDKSVELHIQQDILVI"
BASE COUNT          719 a          279 c          477 g          630 t
ORIGIN      
        1 cccagtcagt gtgatggcaa aagcacagaa aggtagcgtc attaattcca ttaccttcac
       61 tgatagttta actgatgaaa ccgatgataa acatgatttt actaatacag gtgatctgaa
      121 gggcagtacg ccttctatca tgacatttcc gtatggtgaa tcagatgata ggtatacgac
      181 taactatgtt ggtaatgttc agtcctcaaa aacaaaattt ataaatcttg caacagggtt
      241 aacgaatgat actacgccaa ctatattaat atctcttgat aaggagctga ataataatca
      301 acatattgaa attacacgtt ataaagttga tgttgataac gataatatat tgtatgaagt
      361 agttgatgag ataatcccta gtgaacatgt ggatattaaa ggaaaaaata ttattgttaa
      421 agatcaactg gagcatacct atagtcaata ttataaatat gagtttgtca taaaagataa
      481 tgttgatggt aaagaatctg tgacttcgga gaaagagttt tacttcctgt tagatacaga
      541 tgttgaagca tttgatattc acaaaattga taaaactaaa gacaatatat tattttccgg
      601 tacaggtgaa aataatacgc aagtgatgat taaatataaa acagaacgtg gggaggaaaa
      661 aaatataaaa gtagttgttg atgacacagg aaagtatgaa ataaatctaa atggctggga
      721 catcaaggat gcagatggcg ctgaagtaaa aattgttgat tctgctggta acgttaaatc
      781 aggaaactta tataatatcg cgcgtttgta tgtggatatg aatacaaaca aagctataac
      841 gctggattcg gcttataata taattggctc tcagaaagaa ggaaccgatc ctgctaaagc
      901 tcttatcatg tcagatgata atgactgggt gtatattggt ggagggatat ctccccatat
      961 aggggatcag agtccaggct ctgacaacaa cattgatatg gctggcggtg atgatattct
     1021 gtcatctgtt ggtgcagttt tggatggagc caatattaat atgggtgatg gagatgataa
     1081 actatatacg caggatggtt tcgcttcttc aggtacccga aatattatta tgggagacgg
     1141 gaatgacgtt atttctgttg ataattcatt tggtggaaaa aataccattt ctttaggaga
     1201 gggaaacaac ttatttattg ttggtaacta tgtaaatagt attgctgaaa atgatattac
     1261 ggctggtagt ggtgatgaca gaatagaaat tggtacaaat ctggatggta aaaacaaaat
     1321 tgatcttggg gatggtgaca acactattca ggttggagga tacataacca actcacatac
     1381 cattactggg aatagtggtg atgatattat ctatgtcgct acaaatatag atggttctgg
     1441 ctcatttaat ttaggtgacg gtaataacaa ctttatagtc ggaggatata tccaggggaa
     1501 aaacacaatt gagatgggtt ccggtgatga tacggtttct gtttccactc gtattgcaga
     1561 taatgttaaa atccaactta atgccgggga tgacagtgtg tatgcaggtg gattgttaaa
     1621 caaggcaatt gttgacctcg gggatgggga tgatgtcgtc acattgagtg gaatatcaga
     1681 taatggtaag cgtaataata tggaggagtt agtatccact aatgcaatgc ttacaggagg
     1741 ggaaggaaac gatactctga agattaatgg ttctttcaaa ttgttaaata tgaaaaatat
     1801 atctgggttt gaaactattg atcttgggga aagttcagag aatcatctgg atgtcggtat
     1861 aaaatcggat atgctggata tttcatcgtc atctggggtg aaaattttta ctatcatggg
     1921 tggggcggga aatacagttg atttaggtaa ggcaaatata acgtctcaca attcagtaga
     1981 acaaggaaat tatgctgatt catggtataa aggagatact gtggacggat acacaacgta
     2041 tactcctgta ggtgataaat ctgttgaact ccatatccaa caggatatat tagtaatata
     2101 atttt
//
LOCUS       DAZGSP010000264         2040 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZGSP010000264 DAZGSP010000000
VERSION     DAZGSP010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.266"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            353..1843
                     /locus_tag="ACHK74_005264"
     CDS             353..1843
                     /locus_tag="ACHK74_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFP0223898.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTTWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEALQKNRGEKPVRYKFI
                     FNDATTAAIKDQLCGQWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGGSLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLPELNACYSQKLIRWHEQSFGL"
     gene            complement(1965..>2040)
                     /locus_tag="ACHK74_005265"
     CDS             complement(1965..>2040)
                     /locus_tag="ACHK74_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223899.1"
                     /translation="WAVARKWEKRELELMIDEYLLYAC"
BASE COUNT          551 a          438 c          494 g          557 t
ORIGIN      
        1 aggaactgtc atcttcttta ttttattctt cttcaggagg gctgttttct gaaagcgtta
       61 ctccggggaa actgtttatg tttattcccg gcaggaaacc cgtacctgca ataccctgga
      121 tgatgctttt tgctggtacg ttgttgtgtg aaaattaatc aacatgtgtg ctccggagag
      181 cgcccgtaga cgcacagatt cattctgaca ttctttagcg acaaaatatc gagtggttat
      241 tatttaacct gaaaatcgac agaaattttt atcctgtatt gattctgctg ctatttttgc
      301 tattagtagc aatagttgcg gtactttatg ttctgtgtgt tgtgaggtca ttatgccata
      361 ctttcagtat cctgatgagt ttcccctgag ctcgctgcct ccactcattc gtgatgccgt
      421 tattgaagct cagcaaatca cgcaggctcc cctggggctt gtggcagcat ctgccctggg
      481 cgctgtttca cttgtctgcc agaatctgat tgatgtctgc agactgaata cactacgggg
      541 gccggtttct cttttcttac tgacgcttgc tgagtccgga gaacgaaaaa ccgcagtgga
      601 caaattatta atggagcccc tgtaccagca ggagatgctg ttatattcca gacacaaaaa
      661 tgaactgacg acatggaaaa ataaggaaga gcttttaaag gcgcagaaaa aagcattgtt
      721 gtcaaaactg aataaagaac tccgcaaagg cgcggatgag tcagaaacgc tcagacaact
      781 ggaagcgctt cagaaaaaca gaggggaaaa acctgtaaga tataaattca tttttaatga
      841 tgccacgaca gcagcaatta aagatcagct ttgtggtcag tggcgttcag tgggtatcat
      901 gtctgatgaa gccggtataa tatttgatgg gtataccctg tctgagctgc cttttattaa
      961 taaaatgtgg gatggctccg tattgtcggt ggacagaaag aatgaacccg aacagatgat
     1021 tgaaaatgcc agaatgacat tatccctcat ggtacaaccc ggtctttttg accgatatat
     1081 ggagcgaaaa ggttctgttg ccagggacag tggttttctt gcccgttgtc taatcagtaa
     1141 acctgccacc acgcagggga aacgctttat taacggagct gtaaccccgg gaggatcatt
     1201 aacggcattt catgaacgat taatggaact ggccagaggc agtattgaaa aatccagtaa
     1261 ggatgaacgt tattgtctgc atttttctcc tgaagcgcaa aaaatattta ttgatcacta
     1321 taatgttctg gagcaggatt tgtctccttc cggaccctta agccagtttc gggggcatgt
     1381 ttcaaaaaaa acagaaaaca ttgcgagaat tgctgcgtta ttgcagtatt tcagtcaggg
     1441 tgaagggaaa atctctgccg atatcatgac gtctgctgtc gtaataagtt cgtggtacac
     1501 agatgaatat aaaaaattat ttgcgttacc tgatgaatct gaactgcagc tgcaggacgc
     1561 ccgggagctg ctcgactggc tcattgagga gtgccgcgga gaatgcccgc cccgtgtcag
     1621 gaaaaattat attctccagt gtggcccggg ccggttcagg aacaggaaaa agctgaacgt
     1681 cctgctgaac attcttgcat ctcaactcag gctctctgtc gtgcaggaag gaaaaaccac
     1741 ctatgtcctg ctttccgaaa taaacaatat cacgctgcct gaactgaatg cgtgctacag
     1801 ccagaaactg ataagatggc atgagcaatc ctttggctta taatgttccc tgttgctgag
     1861 acggattatt cagtggtggt atcgtagtat gcccgtcacc ccccgcttct gcatgagggt
     1921 gacggaggaa aagcaagtgt gcggaggccc gggagcgtta tgaatcaaca ggcgtataac
     1981 aggtactcgt caatcatcag ttccagctcg cgcttttccc atttgcgcgc cacagcccag
//
LOCUS       DAZGSP010000265         2006 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZGSP010000265 DAZGSP010000000
VERSION     DAZGSP010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.267"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..394
                     /locus_tag="ACHK74_005266"
     CDS             <1..394
                     /locus_tag="ACHK74_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFP0223900.1"
                     /translation="ATFEASESFFSDSKDSGMEYISGKQRYRDSLTSASGSMKRPKRM
                     LVTGYCCRRCKGLALSRTSRRLSQEVTERFYVCTDPGCGLVFKTLQTITRFIVRPVTP
                     DELAERLHEKQELPPVRLKTQSYSLRLE"
     gene            468..1040
                     /locus_tag="ACHK74_005267"
     CDS             468..1040
                     /locus_tag="ACHK74_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFP0223901.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTVLAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDVA
                     SVDDSREKFTPAQNMIFSRKSAQLASHQSV"
     gene            1350..1661
                     /locus_tag="ACHK74_005268"
     CDS             1350..1661
                     /locus_tag="ACHK74_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008807100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223902.1"
                     /translation="MRDKFIDAIHSCLKIQLTFYSKEDNATITRLTAPMDFGPSRRAH
                     DKSDRFHFWDYESDKKSHVLSLQPEAIVSLVVIPQNFHPQEFVSWTPNWFIARDWGQY
                     S"
BASE COUNT          572 a          460 c          455 g          519 t
ORIGIN      
        1 tgcgacattt gaagcgagtg aaagcttttt ctctgacagt aaagattcag gaatggaata
       61 catttcaggc aaacagagat accgcgattc actgacgtca gcctccggca gtatgaaacg
      121 cccgaaaaga atgcttgtta ccggatattg ttgtcggaga tgtaaaggcc ttgcactgtc
      181 aagaacatcg cggcgtctgt ctcaggaagt caccgagcgt ttttatgtgt gcacggatcc
      241 gggctgtggt ctggtgttta aaacgcttca gaccatcacc cgcttcattg tccgcccggt
      301 cacgccggac gaactggcag aacgcctgca tgaaaaacag gaactgccgc cagtacggtt
      361 aaaaacacaa tcatattcgc tgcgtctgga atgagggctg ccggttaacc ccggccgtcg
      421 ccgcacaccg tatttttatt cttcagcatg atgagaaaga gataacgatg gaaagcacag
      481 ccttacagca ggcctttgac acctgtcaga ataacaaagc agcatggctg caacgcaaaa
      541 atgagctggc agcggccgaa caggaatatc tgcggcttct gtcaggagaa ggcagaaatg
      601 tcagccgcct ggacgaatta cgcaatatta tcgaagtcag aaaatggcag gtgaatcagg
      661 ccgccggtcg ttatattcgt tcgcatgaag ccgttcagca catcagcatc cgcgaccggc
      721 tgaatgattt tatgcagcag cacggcacag tactggcggc cgcactggca ccggagctga
      781 tgggctacag tgagctgacg gccattgccc gaaactgtgc catacagcgt gccacagatg
      841 ccctgcgtga agccctcctg tcctggcttg cgaagggtga aaaaattaat tattccgcac
      901 aggatagcga cattttaacg accatcggat tcaggcctga cgtggcttcg gtggatgaca
      961 gccgtgaaaa attcacccct gcgcagaaca tgattttttc gcgtaaaagt gcgcaactgg
     1021 catcacatca gtctgtgtaa aattccccga aaatccgcct gtttttactg aaaaaagcca
     1081 tgcatcgata aggtgcatgg ctttgcatgc gttttcctgc ctcattttct gtataccagg
     1141 ccacgcccgg cacggcctga gcgtgtcagt gcaactgcat taaaaccgcc ccgcaaagcg
     1201 ggcgggcgag gcggggaaag cactgcgcgc aaggctgata acataaataa aatacaatga
     1261 actcattacg ttttattaaa tgccgcaatg atcacacaca ataatctatc aatagaggat
     1321 attaatttag taactacaaa ggagaacgta tgcgcgataa attcattgat gccattcata
     1381 gttgtttaaa aattcagcta actttttatt caaaagaaga taacgcgaca atcacaagat
     1441 taactgcgcc tatggacttc ggtcctagtc gtagagctca tgataaaagt gacagattcc
     1501 atttttggga ttacgaaagc gataaaaaaa gtcatgttct cagtttgcaa cctgaagcga
     1561 ttgtatctct agttgtaatc cctcaaaact ttcatccgca agagtttgtt agctggacgc
     1621 caaactggtt tattgccaga gattggggcc agtactctta aatacatgag gctacatacg
     1681 tagcctttta aatggaatct ccagggaata cgcgcgtaca ttacccgcct ccaaaagccc
     1741 acactttaaa aacgcaggca tcattatgat ttaattgatt tttattatct tctttgatgc
     1801 ttaaattgat gttatactgt ttttatatac agataccagg gcagataagg aggataatat
     1861 gttaaataag cttgccaaat atcttcttac tgccagctca gttgctccgg tattttttac
     1921 cttagctttt ctttcatgta tatcaaaaca ctataaattt atgattgctt acttatcatt
     1981 gtgtgcaatt atacttttac tgtgtg
//
LOCUS       DAZGSP010000266         1945 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZGSP010000266 DAZGSP010000000
VERSION     DAZGSP010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.268"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..72
                     /locus_tag="ACHK74_005269"
     CDS             <1..72
                     /locus_tag="ACHK74_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFP0223903.1"
                     /translation="LLTYLTNLYFKIKEDRRKAARGD"
     gene            76..633
                     /locus_tag="ACHK74_005270"
     CDS             76..633
                     /locus_tag="ACHK74_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223904.1"
                     /translation="MNHEEMNQRFSRLENEIAELNKKLSTLMPSEDEKKRRDEQFAAF
                     DDYCRKVMSRNLAECFSIHNDNFSELEWECNRPSFVVSGDGVKITISENGKVTPPSHQ
                     HSEELIEFAIDYLKNNKKQGLMQRIGRCMGYLQVAAEIEALASGADKDSVVREALLRD
                     FDHPPFKKVPAYWLHPGLTYLKVRI"
     gene            670..1203
                     /locus_tag="ACHK74_005271"
     CDS             670..1203
                     /locus_tag="ACHK74_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0223905.1"
                     /translation="MNTKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GVGIWTICRGATRVDGNPVIPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFTSTFYKRINAGDRRGVCEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1722..1907
                     /locus_tag="ACHK74_005272"
     CDS             1722..1907
                     /locus_tag="ACHK74_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP0223906.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
BASE COUNT          520 a          408 c          552 g          465 t
ORIGIN      
        1 ctgctgacat atctgacgaa cctgtatttc aagattaaag aagaccggcg taaggcggca
       61 cggggagatt aggtgatgaa ccatgaagaa atgaatcagc gcttcagtcg tctggaaaat
      121 gaaattgctg aactgaataa aaaactgtcg acgctgatgc cttctgaaga tgaaaaaaaa
      181 cgccgcgatg agcagtttgc tgcgtttgac gattattgtc ggaaagtgat gagcagaaat
      241 ctcgcagagt gtttcagtat tcataatgat aatttcagtg agctggaatg ggagtgtaac
      301 cggccatcct ttgttgtatc cggtgatggt gtgaaaataa ccatctcaga aaatgggaaa
      361 gtaacacctc catcgcacca gcacagtgag gagctcattg aatttgccat tgattacctg
      421 aagaacaata aaaaacaggg gctgatgcag cgcattggtc gttgcatggg gtatctgcag
      481 gtagccgctg agattgaagc gctggccagt ggtgcggaca aggattcagt tgtgcgggag
      541 gcacttcttc gtgattttga tcatccgccc tttaaaaaag tgccggctta ctggcttcat
      601 cccggactga cttatctgaa agtgcgtatt tagtgggcct gggacagcgg ctgaatattt
      661 aatatatcca tgaacacaaa aatcaaatac ggcctgtcgg ctgccgttct ggcgttgatt
      721 gccgcagggg cttctgcgcc tgaaatcctc gaccagtttc tggatgaaaa agaaggtaac
      781 cacaccacgg cataccgtga tggtgtgggt atctggacca tctgccgtgg agccacccgg
      841 gtggatggta atcctgtgat tcctggcatg aagctgtcga aggaaaaatg cgaccaggtt
      901 aacgccattg aacgggataa ggcgctggca tgggtggaga aaaacatcag agtgccactg
      961 actgaacccc agaaagcggg tattgcgtca ttctgtcctt acaacattgg cccgggtaag
     1021 tgtttcacgt cgacgtttta taaacgaatt aatgcaggag atcgcagggg agtgtgtgaa
     1081 gcgattcgct ggtggattaa ggacggtggc agagactgcc gtattcgctc aaataactgt
     1141 tatggtcagg tatcccgtcg tgaccaggag agcgcgctgg cgtgctgggg aatcgacaga
     1201 taagcagaat attttgctga aaaatgacgt tggtcaacgc ggacggataa cacgaaatcc
     1261 tgcgaactgg caaaacctaa gtgaataaaa gtaaaaaaac cgtttgttag cagcaggtgg
     1321 ggttttgtgt ttcctgactc cggaaaagtc aaaggagaaa gtgtgtttga ttttagcaaa
     1381 ctgattcggg agatttgagt gatggctgaa aaattatcta cctggaagtt cattcttatc
     1441 tggctggtgt ttgtgattat ggtctccggt tatttcatcg gtcagatacg ctggtggtga
     1501 aatgaaccgc gttctgtgtg tggtcatcat tgccctgctg gtggcctgtg gtgcgcttag
     1561 tctggggctg aatcattacc gtgataacgc catcacctac aaagcgcagc gcgataaaaa
     1621 agccagagag ctggagctgg caaacgcaac cattactgat atgcaggtgc gccagcgcga
     1681 tgttgctgcg ctcgatgcaa aatactcgag ggaattagct gatgcgagag ctgaaaatga
     1741 aactctgcgt gctgatgttg ccgctggtcg taagcgcctg cggatcaacg ccacctgctc
     1801 cggtaccgtg cgtgaagcca ccggcacctc cggcgtggat aatgcaaccg gcccccgact
     1861 ggcagacacc gctgaacggg attatttcat cctcagagaa cggttgatga caatgcagaa
     1921 gcagctggaa ggggcacagg actat
//
LOCUS       DAZGSP010000267         1932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZGSP010000267 DAZGSP010000000
VERSION     DAZGSP010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.269"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            467..>1932
                     /locus_tag="ACHK74_005273"
     CDS             467..>1932
                     /locus_tag="ACHK74_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFP0223907.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSSPALASVVGNTLPWQTYRDFAENKGAFHAGATNIPLYGKNGAVSGRLDK
                     APMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVRFGYADDTTYRLVDRNEHWRDF
                     HTPRLNKLVTEVAPVSVTDAGTGKGVYQNRSRYPVFYRMGSGTQYTGAASGALTRIAD
                     AYVWRTGGTVGSPVISDWSLVSNPGNLYQGVNGPLASYGTSGDSGSPLFAWDAVKKQW
                     VFVAVLHGYAGENGKTNWFTVIPAGDVNNTIKQDNSGTVVPAVAGGDIVWKYSQGSGE
                     GTLSQSGQVWTMNGFRGGSLNDGKDITFGGKGTVVLKDDVVQGAGSLTFNGDYTVRPE
                     GNQTWVGGGIIVNDGHRVDWMVNGLAGDALHKIGKGTLVVAGSGENPGTLNTGDGTVI
                     LAQKADAAGRVRAFSEVSIVSGRPVVVLQDSHQIEGDR"
BASE COUNT          460 a          375 c          624 g          473 t
ORIGIN      
        1 taaacacgaa gacggtgaac acctttctgt atatgggact tggtaaaaac ggtcttaagt
       61 acaggagtgc gaagcacctg gttggacgtg cgtagccgga atgcatattc gtgagtcggg
      121 taatgagagt tgaacggtgg cagtgagaat ggaacagaaa aaaatcagat gcggaaggcg
      181 gaaatacaca gcgggtgcga tgtgcttcat gctgtttgta tggctgggtg gtgtggtgac
      241 attctgtgtg ctggccgctc tgataatgag gtgaaaacgg aagagagttt acttccttaa
      301 ttcgaacttt ctgataactg agaacttgtt aaatgctcgt gggtttatac atttaaatca
      361 attggttaat gctgacttca tctttatcag tcaaagtttg ttttagtatg atgattgtgc
      421 ccactgttta ttgttgtccg ggagaaaata cagggaattc cagggaatga acaaaatata
      481 ctcactcaga gttaatgaca gaaaagaact ggttgctgta ccggaaacgg tggggggcag
      541 gaaaaagtcg tccggcattc ccggtgcggg gatgctttcc cgtcttcttc tggcctcagg
      601 agccgttgcc ggcgttctgt tttcttctcc tgcgctggca tccgttgtcg ggaatactct
      661 gccatggcag acctacaggg attttgctga aaacaaaggg gcgtttcatg ccggggccac
      721 aaatattcct ctttatggta agaatggggc tgtcagtggg cggctggaca aggctccgat
      781 gatggatttc agtgttgtgg accagatact gggggtggca acactgattt ctccgcagta
      841 tgtggcgggg gttaagcata acggcagcta caatacggtg cgttttggtt atgcggacga
      901 tacgacatat cgccttgttg accgtaatga gcactggcgt gattttcata ctccccggct
      961 gaacaaactg gtgacagaag ttgccccggt gagcgtcacg gatgccggaa ccggcaaggg
     1021 ggtataccag aaccgaagtc gttatccggt gttttatcgt atggggagtg gaactcagta
     1081 caccggagct gcttccggtg cgctgaccag aatagcggat gcttacgtct ggaggactgg
     1141 cgggacggtg ggaagtccgg tcatttctga ctggagtctg gtgtcaaacc cgggaaatct
     1201 ttaccagggt gttaatggtc cgctggcgtc ttacgggaca tcaggagaca gtggctcacc
     1261 gctttttgcc tgggatgcgg tgaagaagca gtgggtattt gttgctgtac ttcatggtta
     1321 tgccggtgag aacgggaaga cgaactggtt tacggtgatt cctgcagggg atgtgaataa
     1381 cacgataaag caggacaatt caggcacagt tgtgccggca gttgccggtg gtgacattgt
     1441 gtggaagtac agtcagggaa gcggagaggg aaccctttct cagagcgggc aggtatggac
     1501 gatgaacggc ttcaggggag ggagtctgaa tgacgggaaa gatatcacct ttgggggtaa
     1561 gggcacggtt gttctgaagg atgatgttgt ccagggggcg ggttcgctca cgtttaatgg
     1621 tgattatacg gtcagaccgg aagggaatca gacctgggtg ggcgggggaa tcatcgtgaa
     1681 cgacgggcac cgtgttgact ggatggtgaa cggacttgct ggagacgcac ttcataaaat
     1741 tggcaaggga acactggtgg tggccggcag tggtgaaaat cctggcaccc tgaatacggg
     1801 ggacggaacg gtcattctgg cacagaaggc tgatgctgca ggcagggtcc gggcattcag
     1861 tgaagtcagt attgtcagcg gacgaccagt tgtggtgctg caggacagtc atcagattga
     1921 aggggaccgc at
//
LOCUS       DAZGSP010000268         1923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZGSP010000268 DAZGSP010000000
VERSION     DAZGSP010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.272"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            21..1183
                     /locus_tag="ACHK74_005274"
     CDS             join(21..282,282..1183)
                     /locus_tag="ACHK74_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HFP0223908.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
     gene            1335..>1923
                     /locus_tag="ACHK74_005275"
     CDS             1335..>1923
                     /locus_tag="ACHK74_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223909.1"
                     /translation="MIFRDSLRVLPYGRVDNDFFQIEERRSWNAGRYYWSNRRIFGYI
                     GITQSSNKELKDKSGREGFIRNQAARELKTIISNLLTELADRFFGSRSDDRKELLEQV
                     KREKELRKSAQQQARKSTQKSFSEALKNQTPVLDASLEAVKRLKTKLDKTDGSLDLNY
                     LKIIDSDLTNLDALRSEIKTPIKPPKLGMYEEKYRD"
BASE COUNT          557 a          420 c          491 g          455 t
ORIGIN      
        1 ctcagtcaga ggtgactcac atgacaaaaa cagtatcaac cagtaaaaaa ccccgtaaac
       61 agcattcgcc tgaatttcgc agtgaagccc tgaagcttgc tgaacgcatc ggtgttactg
      121 ccgcagcccg tgaactcagc ctgtatgaat cacagctcta caactggcgc agtaaacagc
      181 aaaatcagca gacgtcttct gaacgtgaac tggagatgtc taccgagatt gcacgtctca
      241 aacgccagct ggcagaacgg gatgaagagc tggctatcct ccaaaaggcc gcgacatact
      301 tcgcgaagcg cctgaaatga agtatgtctt tattgaaaaa catcaggctg agttcagcat
      361 caaagcaatg tgccgcgtgc tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg
      421 gcggacaagg ataagcacgc gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc
      481 ggcttttacc cggtcaaaac agcgttacgg tgccccacgc ctgacggatg aactgcgtgc
      541 tcagggttac ccctttaacg taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag
      601 ggcaaaggcc tcccggaagt tcagcccggt cagctaccgc gcacacggcc tgcctgtgtc
      661 agaaaatctg ttggagcagg atttttacgc cagtggcccg aaccagaagt gggcaggaga
      721 catcacgtac ttacgtacag atgaaggctg gctgtatctg gcagtggtca ttgacctgtg
      781 gtcacgtgcc gttattggct ggtcaatgtc gccacgcatg acggcgcaac tggcctgcga
      841 tgccctgcag atggcgctgt ggcggcgtaa gaggccccgg aacgttatcg ttcacacgga
      901 ccgtggaggc cagtactgtt cagcagatta tcaggcgcaa ctgaagcggc ataatctgcg
      961 tggaagtatg agcgcaaaag gttgctgcta cgataatgcc tgcgtggaaa gcttctttca
     1021 ttcgctgaaa gtggaatgta tccatggaga acactttatc agccgggaaa taatgcgggc
     1081 aacggtgttt aattatatcg aatgtgatta caatcggtgg cggcggcaca gttggtgtgg
     1141 cggcctcagt ccggaacaat ttgaaaacaa gaacctcgct taggcctgtg tccatattac
     1201 gtgggtagga tcacgcaggg gtcggcccat ttgagcttca gataggaacg tttgaattcc
     1261 tttcacaaaa tacatcacat actgaacgtg aacatttcca ctttgatctt aaggccaaaa
     1321 agtatgcagg actaatgatt tttagggata gcttgagagt attaccttat ggtcgagtag
     1381 ataatgattt cttccagata gaagaaagac gttcatggaa tgcagggcga tattattggt
     1441 ctaatagaag gatttttggt tatattggaa ttactcaatc cagtaataaa gagctgaaag
     1501 ataagtcggg gagagaggga ttcataagaa accaggctgc aagagaactg aaaactatta
     1561 tatctaattt gttaactgaa cttgctgata gattttttgg ttcacgttct gatgaccgta
     1621 aagagctttt agaacaagtt aagcgtgaaa aagagttaag aaaatctgct caacaacaag
     1681 ctcgaaaatc aacacaaaaa agtttttcag aagctttgaa gaatcagaca ccagttcttg
     1741 atgcttcctt ggaggctgtt aaaaggctga aaactaagct tgataaaact gatggttcgt
     1801 tagatttaaa ctatcttaaa attatagaca gtgatcttac aaacttagat gcgttgcgca
     1861 gtgaaattaa aacgcctatc aaacctccaa aacttggaat gtatgaagag aaatatagag
     1921 act
//
LOCUS       DAZGSP010000269         1922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZGSP010000269 DAZGSP010000000
VERSION     DAZGSP010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.273"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            818..1690
                     /locus_tag="ACHK74_005276"
     CDS             818..1690
                     /locus_tag="ACHK74_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP0223910.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
BASE COUNT          520 a          505 c          421 g          476 t
ORIGIN      
        1 ccctccctgc cggcacagat aaaatgcacc attttttctt caaatacttc tgtataatcg
       61 ccagccaatg atgagaatgg ataaaaacaa cacttcttcg acaaacacaa aaaccagcct
      121 tcattttcac atcatcatcc agttttaatt aaaccctgac gagcgacagt atttcagttc
      181 aggaattaaa agccagctat tactcagtaa aaacaccacc aactaataat atctcagcag
      241 atatttcctg accaggatga cacatttatt aaagattttt aacaggacac ttcacattac
      301 tgaacgctat tccgacagta tcactacaca aataaaaaag ggaactggat aaatattcag
      361 gccggaagaa tggtgtcaca aaagcattta atcattacgc atgaccagga agggaaacac
      421 ttaacaacat ggtatcaaca gatatcatta cgtgcgcact cttataaaaa tacacagcag
      481 aagcaagata actctaaacg taaatttaat atctcccact gacatctccc ccacaataca
      541 ctgaaaaata ctcacagcag taaacacaga cacacctgat aatcatgcag ccgataaata
      601 ttttcccctt tacggagaaa tcagcctgct gtttaacaat aacggcgtat tttcatggaa
      661 ggagatcacc cagtaacgga taactcctgc cctccttcat gttaatacgc aacatcccca
      721 tcgattccaa acaatttcag acagatatta tctccgtgga tcccccgcca cctttccggt
      781 gcgcggggtt ttgtcttttt tcaccgggaa tacatgtatg aatccgtctg atgccattga
      841 ggcaattgaa aaaccccttt cttccctgcc ttactcgctt tcacgtcaca tcctggaaca
      901 tctgcgcaaa ctcaccagtc acgaacccgt gattggcatt atgggtaaaa gcggggccgg
      961 taaatcttca ctctgtaatg cactgtttca gggggaggtc acaccggtca gtgatgttca
     1021 cgccggcacc cgggaagtgc agcgcttccg tctgagtggt cacggtcaca gcatggttat
     1081 cactgacctg cccggggtgg gcgagagccg ggacagggat gcagagtatg aggccctgta
     1141 ccgtgacatt ctgcctgaac ttgacctggt actgtggctg attaaagccg atgaccgtgc
     1201 cctgtctgtg gatgagtatt tctggcgaca catcctgcag tgcggacatc agcaggtgct
     1261 gtttgtggtg acgcaggccg acaaaacgga gccctgccat gaatgggata tggccggtat
     1321 tcagccttct cctgcacagg cacagaatat tcgcgaaaaa acggaggcgg tattccgtct
     1381 gttccggccc gtacatccgg ttgtggccgt atcggcccgc accggctggg aactggatac
     1441 gctggtcagt gcgctcatga cagcgcttcc cgaccatgcc gccagtcccc tgatgacccg
     1501 actgcaggac gggctgcgca cggaatctgt ccgctctcag gcccgtgaac agtttaccgg
     1561 tgcggtggac cggatatttg acacagcgga gagcgtctgt gttgcctctg ttgtccgtac
     1621 ggccctgcgc gctgttcgtg acacggtggt ctctgttgcc cgtgcggtat ggaactggat
     1681 tttcttctga acctgtcgtg gatgatgtcc tccctgcctc tgggtctgct cacaaaagcg
     1741 ctgttttcgt tactgtctct cttgtccgtg caatagcttg acaatagaat aaaacgatcg
     1801 atatctattt tatcgatcgt ttatatcgat cgataggcta ataataacct ttgtcagtaa
     1861 catgcacaga tacgtacaga aatacattca gggaacaaca gaaccacacg tcagaaactc
     1921 cc
//
LOCUS       DAZGSP010000270         1915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZGSP010000270 DAZGSP010000000
VERSION     DAZGSP010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.274"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            112..783
                     /gene="queE"
                     /locus_tag="ACHK74_005277"
     CDS             112..783
                     /gene="queE"
                     /locus_tag="ACHK74_005277"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFP0223911.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     repeat_region   1125..1761
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1125..1151
                     /rpt_unit_seq="gttccccgcgccagcggggataaaccg"
BASE COUNT          515 a          445 c          477 g          478 t
ORIGIN      
        1 gatatgtttc gcatttttct ttaaatctgc ctgtaacaga atgttaataa gacatgattg
       61 ttgtggcagg atctgcaata attaacaatt atccacttca cagagaatgc tatgcagtac
      121 ccgattaacg agatgttcca gaccctgcaa ggtgagggtt actttaccgg cgtccccgcc
      181 atttttattc gtttacaggg atgcccggtt ggctgtgcct ggtgcgacac caaacacacc
      241 tgggaaaagc ttgaggatcg ggaagtctcc cttttcagca ttctggcgaa gaccaaagag
      301 agtgataagt ggggggcggc gagcagtgaa gatttgctgg ctgtcattgg tcggcaggga
      361 tataccgcgc ggcatgtggt gattacgggt ggtgagcctt gcattcatga tttgctgcca
      421 ctgactgacc tgctcgaaaa gaacggtttt agctgccaga tcgaaaccag tggaactcat
      481 gaagtacgct gcacaccgaa tacctgggtt accgtatcgc caaagctgaa catgcgcggc
      541 ggctatgacg tgttgtcaca ggcactggag cgagccaacg aaatcaagca tccagtgggg
      601 cgcgtacgtg atattgaagc actggatgaa ctactggcaa cgctgaccga tgataaaccg
      661 cgagtcattg cactgcagcc gattagccaa aaggatgatg ccacacgttt gtgcattgaa
      721 acctgcattg cgcgtaattg gcgtttgtcg atgcaaacac ataaatatct aaatattgcc
      781 tgattaaaca tttataagcg ttataaatgg gtggaacctg taaagacttc tactcattta
      841 tattgtttgt cgcctctgaa aactcctcca ttttacccat ccagggctaa tcattagcat
      901 tctctacaaa ttctgtggca ttaatttttc gctggagtga aaattattgc ggtaaagttt
      961 ggtagatttt agtttgtata gagttatttt aaatatttac ctttttaatc aatggattaa
     1021 gtgctcttta acataatgga tgtgttgttt gtgtgatact ataaagttgg tagattgtga
     1081 ctggcttaaa aaatcattaa ttaataatag gttatgttta gagtgttccc cgcgccagcg
     1141 gggataaacc gcgtagtgct gctgtatcga tgcctgatgc gccgtgttcc ccgcgccagc
     1201 ggggataaac cgaattcggg ccacacgcgc gcccgttcat gcgagtgttc cccgcgccag
     1261 cggggataaa ccgcgcaccg agggggaaaa ctccggcgca ggaatgtgtt ccccgcgcca
     1321 gcggggataa accgaacacc tacgcaaacg agcagtggct aaaagggtgt tccccgcgcc
     1381 agcggggata aaccgatgtc gccagaacaa aaacaaaaaa cattatagag ttccccgcgc
     1441 cagcggggat aaaccgttat cgcaccgtgt taaaccggca gaaaaacaga gttccccgcg
     1501 ccagcgggga taaaccgcgt gccttcgact ttgcggatca cgtcgtcttg agttccccgc
     1561 gccagcgggg ataaaccgac ggggacgaat cacagcagcg ttatcgtgat gagttccccg
     1621 cgccagcggg gataaaccga aaccggacga gggcgcgcta aaacgtgatt agagttcccc
     1681 gcgccagcgg ggataaaccg cctctcaatt tgtaacgtct gcaatgtcag gcgagttccc
     1741 cgcgccagcg gggataaacc accatataac ccgttatctc tttctcaagt ttttatatta
     1801 gcagtacttg taataagcaa catatccacg taacacctca tgttcaaaat agttctccat
     1861 gccagagagg ttcacaatta tcgatacaaa aaattaaatt taatcaaagt gttat
//
LOCUS       DAZGSP010000271         1905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZGSP010000271 DAZGSP010000000
VERSION     DAZGSP010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.275"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..548
                     /locus_tag="ACHK74_005278"
     CDS             <1..548
                     /locus_tag="ACHK74_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFP0223912.1"
                     /translation="ALEKNQPGKPVRVINPDDESIRIFSWLADNFPDLQYFKLQLLDA
                     SNLRLTVSKQRNAITQQLIDNLIKGLLQTMPYASNISIAVLDDNVLESQAIETLSAIG
                     LSYEKYKTANNVYFNIIGTLSDSELNKINNYVDEYYKQWGKQYVRFNVNLKNQDTNNS
                     SFSYGGNRFEKSQGSNWTFQE"
     gene            562..802
                     /locus_tag="ACHK74_005279"
                     /pseudo
     CDS             562..802
                     /locus_tag="ACHK74_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            822..1153
                     /gene="sctI"
                     /locus_tag="ACHK74_005280"
                     /pseudo
     CDS             822..1153
                     /gene="sctI"
                     /locus_tag="ACHK74_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            1150..1884
                     /locus_tag="ACHK74_005281"
     CDS             1150..1884
                     /locus_tag="ACHK74_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFP0223913.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSSASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLA
                     LISVATCWLLWKYRAILTNLVRLKIK"
BASE COUNT          653 a          309 c          380 g          563 t
ORIGIN      
        1 aagcactgga aaaaaatcaa ccaggaaagc cagtacgggt gataaatcct gatgatgaat
       61 cgatacgaat tttttcatgg cttgctgata atttccctga tttacaatat tttaaacttc
      121 aactattaga tgccagtaat ctaagattga cagtgagtaa gcaacggaac gctatcacac
      181 agcaactaat cgataatctt attaaaggat tactgcaaac tatgccatat gccagcaata
      241 taagtattgc ggtattagat gataatgtat tggaaagtca ggctattgaa acgttgtcag
      301 cgataggtct ttcttatgaa aaatataaaa cagctaacaa tgtgtatttc aatatcattg
      361 gtacgttaag tgacagtgaa ttaaataaaa ttaataacta tgttgacgaa tattataaac
      421 aatggggtaa acaatatgta agatttaatg tgaatttgaa aaatcaggac acaaataata
      481 gttcatttag ctacggaggt aaccgattcg agaagtctca aggtagcaac tggacatttc
      541 aggaataatt gaggaggact tatggcagat tggaatggtt atattatgga tatcagcaat
      601 caatttgatc agggcgttga tgatctgaac cagcaagttg aaaaagcgtt ggaggattta
      661 gccaaccaat ccctccgaca cgaaatttct tgccgaatat caaagtgcat tagctgagta
      721 tacattatat cgaaatgcgc aatctaacgt tgtaaaagcg tataaagatc ttgattcagc
      781 aatcatacaa aacttccgct aaactggata aggattttat tatgtcagta agcaatatgc
      841 cacctattga tagagctgag caatcaactg ctcatgaaat acagcaagcg aaagtgattg
      901 atttaaatga ccgggttctc aatcttgaca acccggatga taaaatgatc tccgcttttg
      961 ccaactatgc tgtacaaact gaaaattggc agcaaaatgc gttacaagca ttaaggtctg
     1021 ataaagaggg gttaactcct gaaaaattat tagtcttgca ggatcatgtg cttaattata
     1081 atgtcgaggt ttcattggtg gaacattagc aagaaagatt gtcgctgcag ttgagacgtt
     1141 gacccgctca tgaaatatat atcattatta ttgtttatcc tgctgttgtg cggctgcaag
     1201 cagcaggaat tattgaacca tcttgatcaa cagcaagcta atgatgtcct tgctgtttta
     1261 caaagacata atattaatgc tgaaaagaag gatcaaggca aaacaggatt ttcaatattt
     1321 gtcgaaccaa cggattttgc ttctgcggtg gattggctaa aaatctacaa tctaccgggt
     1381 aagcctgata ttcaaatatc gcagatgttc cctgcagatg cactcgtttc atctccacga
     1441 gcggaaaaag caaggctata ttctgctatt gagcagcggt tagagcaatc tctcaaaatt
     1501 atggatggca ttatttcttc gagagtacat gttagttacg atgttgataa tggcgatagt
     1561 ggtaaaactg ctttaccaat tcatatttct gtacttgcag tatatgaaaa agatatcaac
     1621 ccagaaataa aaataaatga tatcaagaga tttattgtta atagctctgc aagcgtacaa
     1681 tatgaaaata tttctgttgt attgtcaaaa cgaagagaca ttattgagca agcaccaaca
     1741 tatgaaatta gcgaacctgt ttttgcttat gataaggcta tgccggtttc tatcttactt
     1801 gcattaatat ccgtagcgac ttgctggctg ttatggaagt atagggccat tctaactaat
     1861 ttagtcaggt tgaaaattaa gtagacatat gagagcgtta tgaat
//
LOCUS       DAZGSP010000272         1900 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZGSP010000272 DAZGSP010000000
VERSION     DAZGSP010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.276"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            78..>1900
                     /locus_tag="ACHK74_005282"
     CDS             78..>1900
                     /locus_tag="ACHK74_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP0223914.1"
                     /translation="MNLRKKHICIAISLVLNPFPASLAAQNTNVVEVDETDKGHQVYG
                     DNWKSIEIGTTKEVKKKDSTDLNFDHNSPVAKGHNSIAIGTSAEAGGEIKDEKGFKTG
                     ESIAIGAKAKATKEQSIAIGGDTKATGWGAIVIGGDDLTPLHGDRFGDKKIPNDYSSS
                     EASGDGSIVVGSRSTAAGDLSVSLGSISSANGTAANALGATAEANGDMATAVGFNALA
                     NASNALAVGSQASAGGEDSLAIGSQSTTGKKSTVALGQGATASAAEGNVAIGADSVDK
                     AATPVSGATIKLKNGGTIEYRGFAGDKAASVVSVGDAGKERQIVNVGAGAVSEKSTDA
                     INGSQLYAIAKTLKDDLDAINPTWKLKDSNGGSKEVGSKNGGTTGNEVTVTGKGDIDV
                     TVDGNGLSIDGTKLAENIKKDAIKIKTGDGKTLTKKLGEEVEFNDAGPIKTKINNNKL
                     EITAETASFTPDGNGGNGKITVSNGDDDKLVTAKKIADAINQSGWKIKAGSENGGKVE
                     GDNKEELINPGDTVTLKAGKNLSVKQSNGDFTFSVVDTPSFSSVQLNGKGNNGSVMLS
                     SEDNKLTLSGGNGAGQNGVVISGVASGLNNKDLAGLKADDA"
BASE COUNT          611 a          327 c          525 g          437 t
ORIGIN      
        1 ccttacataa tattcatgca tgatttttat gctattccga ctgagtggaa gaaaataaag
       61 gagaaaagag gatatatatg aacctcagaa aaaaacatat ttgtatagcc atatcacttg
      121 ttcttaatcc gttccctgca tcactggcag cacaaaacac taatgtagtt gaagttgatg
      181 aaacagataa aggtcaccaa gtttatggtg acaactggaa aagtattgaa ataggtacaa
      241 cgaaggaagt aaagaaaaag gattcaaccg accttaattt tgaccataac tccccggtag
      301 cgaaagggca taactccatt gcgattggta ccagtgcaga agcaggaggt gaaattaaag
      361 acgaaaaagg ttttaagaca ggagaatcca tagctattgg tgccaaggcg aaagcaacta
      421 aagaacagtc aattgcaata ggtggcgata ccaaagctac cggatggggg gctattgtta
      481 ttggtggcga tgatttaacc cctttgcatg gtgacagatt cggtgataaa aaaattccga
      541 atgattactc gtcatctgaa gcatcaggag acggaagtat tgttgtgggc tcaaggagca
      601 cagctgcagg agacctgtct gtgtccctgg gctcaataag ctctgccaac gggacggctg
      661 ctaatgcact gggagccacg gcggaagcta acggggatat ggcgactgcg gttggtttta
      721 atgcgctggc taatgccagt aatgcgctgg ctgttggtag tcaggcctct gccggcggag
      781 aggattccct ggctataggg agtcagagta cgacgggcaa aaagtccacg gttgctctgg
      841 ggcagggagc tacagcctct gccgcggagg ggaacgtggc catcggtgct gattcagtgg
      901 acaaggcagc aactccggtt tccggcgcta ccattaagtt aaagaatggc gggactattg
      961 aatacagggg ctttgccgga gataaggctg catcagtagt atctgttggt gatgcgggta
     1021 aagagcgtca gattgttaac gtgggggcgg gtgctgtatc tgagaaaagt acagatgcca
     1081 ttaacggtag tcagttatat gcgatagcga agacactgaa agatgacctt gatgccatta
     1141 atccgacgtg gaagttaaaa gacagtaacg gtggaagtaa agaagtcggc agtaaaaatg
     1201 gcgggactac aggtaatgaa gtgaccgtca ccggtaaagg ggatattgat gttactgttg
     1261 atggaaatgg gctgagtatt gatggcacaa agcttgctga aaacatcaaa aaagatgcca
     1321 tcaagattaa aacgggagat ggtaaaacac taacgaaaaa acttggtgaa gaagttgagt
     1381 ttaatgatgc cggaccaata aaaacaaaaa ttaataataa caaacttgag ataactgccg
     1441 aaacagccag ttttacgcct gatggaaatg gtggtaacgg gaagattaca gtaagcaatg
     1501 gtgatgacga taagcttgtt actgcgaaaa agattgctga tgccataaac cagtctggct
     1561 ggaaaattaa agctggcagt gaaaatggtg ggaaagttga gggggataat aaagaggagc
     1621 taattaatcc cggtgataca gttacactga aagccggtaa aaatctcagc gtgaaacaaa
     1681 gtaacggtga ttttacattc tctgttgttg atactccgtc attcagcagt gttcagctga
     1741 atggaaaagg aaataacgga agtgtaatgc tctcatccga agataataag ctgacattgt
     1801 caggcggtaa tggtgccgga caaaatggtg ttgttatcag tggtgtggca agtggattga
     1861 ataataagga cctggcaggc ctgaaagcag acgatgcgga
//
LOCUS       DAZGSP010000273         1882 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZGSP010000273 DAZGSP010000000
VERSION     DAZGSP010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.277"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..827)
                     /locus_tag="ACHK74_005283"
     CDS             complement(<1..827)
                     /locus_tag="ACHK74_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HFP0223915.1"
                     /translation="MENPAALLRRLNHYCARALEGAASLCQTRAHAEITPEHWLLKLL
                     EQGEGDLTVLGRRYDWDMDAIWQSLLGWLDNQPRSVRRRPQLAPSLNALLKQAWMVAS
                     LQGEEHIRSVHLLGALTENPHLVRCDGLWPLLTLSHSQLQRLSPLLDAQSDECPERLQ
                     DAEPVLPQGDNVTFIGRPVGADTAGIPSGDLPPVLQSALDKFTRDITASAREGKIDPV
                     SGRDTEIRQMVDILSRRRKNNPILVGDPGVGKTALVEGLALRIAEGNVPESLRPVTLR
                     "
     gene            complement(996..1487)
                     /gene="hcp"
                     /locus_tag="ACHK74_005284"
     CDS             complement(996..1487)
                     /gene="hcp"
                     /locus_tag="ACHK74_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system effector Hcp"
                     /protein_id="HFP0223916.1"
                     /translation="MAIPVYLWLQDDGGADIKGSVDVKDREGSIEVVAQEHCLYIPTD
                     NNTGKLTGTRIHTPFLFTKEIDSSSPYLYKAVTTGQTLKSAEFKWYRINDAGQEVEYF
                     NTKLENVKVVKVNPVMHDIKNPAYEKHNHLEQVELRYEKITWTYKDGNIIHSDAWNER
                     TTA"
     gene            complement(1522..>1882)
                     /locus_tag="ACHK74_005285"
     CDS             complement(1522..>1882)
                     /locus_tag="ACHK74_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP0223917.1"
                     /translation="LFDAGKWTLKPGATKWLVNALVDIKAKAGWLIVVSGHTDNTGDP
                     QRNQALSLKRAEAVRDWMRDTGDIPQSCFAVQGYGESRPVAPNETAEGRARNRRVEIS
                     LVPQADACRLPDITRAP"
BASE COUNT          394 a          581 c          484 g          423 t
ORIGIN      
        1 cgcagggtga cgggtctgag tgattccggt acgttacctt ccgcaatacg cagggcgagc
       61 ccttccacca gagccgtttt ccccacaccg gggtctccca ccagaatagg gttgttcttg
      121 cggcggcggg agagaatatc caccatctga cggatttccg tatcgcgtcc cgataccggg
      181 tcaattttcc cctctctcgc gctggccgtg atgtcccggg tgaatttatc cagagcactc
      241 tgtaacacag gcggcaggtc acctgacggt atacccgccg tatccgcacc gacgggacgc
      301 ccgataaagg tcacattgtc tccctgaggc agcacaggct ctgcatcctg taatctctcc
      361 ggacactcat cagactgcgc atccagcagc ggggacagac gctgcaactg gctatgactc
      421 agtgtcagca gcggccacag cccgtcacag cgaaccaggt gcggattttc cgtcagggca
      481 cccagcagat gcacgctgcg gatatgttct tctccctgta atgaggccac catccaggcc
      541 tgtttcagca gggcattcag ggacggcgca agctgcgggc gcctgcgtac gctacggggc
      601 tggttatcca gccagccgag cagcgactgc catatcgcgt ccatatccca gtcataacgc
      661 cggcccagca cggtaaggtc tccttcgccc tgctccagca gtttcagtaa ccagtgctcc
      721 ggggtgattt ccgcatgggc ccgggtctgg caaagggagg ctgcgccttc cagtgcacgg
      781 gcacagtaat gattaagacg gcgtaacaga gctgccggat tttccatgtt attcactcac
      841 tgtcagaaga ccttcagaca cgctaccgcc cttcgccata acccgggcgc atcaggtctg
      901 tgttcaggat tgagaattct ttcttcagca cccgccaaca gatactgaag aaaaaagaaa
      961 aatgacggac aggataccct gcccgtccgg gagaattacg cagtggtacg ttcattccac
     1021 gcgtcagaat gaatgatgtt accgtcctta taagtccagg tgattttctc gtagcgcagc
     1081 tccacctgct caaggtggtt atgcttctcg taggcgggat tcttgatgtc gtgcatgacc
     1141 gggttaacct tcaccacctt cacgttctca agcttcgtat tgaaatactc cacttcctga
     1201 ccggcatcat tgattctgta ccacttaaat tcagcagact tcagggtctg ccccgtggtc
     1261 accgccttgt aaaggtaggg gctggaggaa tcaatttcct tggtaaacag gaacggcgtg
     1321 tgaatacggg taccggtcag cttaccggta ttgttatccg tcggaatgta aaggcagtgc
     1381 tcctgagcca ccacttcaat actgccctca cggtccttca catccacgga gcctttaata
     1441 tccgctccgc catcatcctg cagccacaga taaacaggaa ttgccataaa gttactccct
     1501 ctcgttgtct tacgcgccct gttacggcgc ccgggtgatg tccggcagac gacaggcatc
     1561 ggcctgcggt accagactga tttcaacacg gcggttacgc gctcgccctt ccgccgtctc
     1621 attcggagcc acgggacgac tctcaccata tccctgtacg gcaaaacagc tttgagggat
     1681 atcgcccgta tcccgcatcc agtcgcgaac ggcctctgcg cgcttcagtg acagggcctg
     1741 gttgcgctgc gggtcgcccg tattgtccgt gtgaccactg accacaatca gccagcccgc
     1801 ttttgcctta atatccacca gggcattcac cagccatttt gtggcgcccg gtttcagcgt
     1861 ccatttaccg gcatcaaaca gc
//
LOCUS       DAZGSP010000274         1845 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZGSP010000274 DAZGSP010000000
VERSION     DAZGSP010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.278"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1845)
                     /gene="espI"
                     /locus_tag="ACHK74_005286"
     CDS             complement(<1..>1845)
                     /gene="espI"
                     /locus_tag="ACHK74_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease autotransporter EspI"
                     /protein_id="HFP0223918.1"
                     /translation="RLMTVSLVLSVTLSALPGKASTVSAEIPYQTFRDFAENKGVFTP
                     GVTGIEINDNNGNKVGVLDVPMLDFSSLSRDGHTTLIHPGYVVSAKHGGLQSVSSATF
                     GYDQIYKIVDNNLAGIDFSAPRLNKLVTEVIPADIQGKDKFNNNRYTAFYRAGVGSQY
                     IRYANGTDKLLQAYTPEKAYLTGGTVGKPYYTHYNGMKMISANPGNTFDKNQGPLASY
                     GQSGDSGSPLYAWDNIDKKWVLAGVTLHNYGVKGARNDWLLIPHDFISQKLQDDLKPI
                     IVASPEENILRWEFDRSRGTGTLSQGEKIFSMTGSVNGNANTGNNLVFSGNEGKIELV
                     SSVEQGAGYLQFDKDYTVLTNNNSTWTGAGIIVGDEANVKWGVNGIAGDNLHKVGSGT
                     LTVNGHGENKGGLKVGDGVVVLEQQPDANQKQQAFSHINIASGRATVKLNGANQVDAD
                     NISWGYRGGKLDLNGYDFTFSRLQAADYGAEISNDNQTDKSIVTLSLSPLKAEEINVV
                     VNNINIMGGTGKPGDLYYTTFDGNYYLLKSNRYGSALFGALNNQSEWQRLGKDKEKAI
                     GLYTQMKMQESAPLSYIYHGKITGNTSVEIPKLAGNDILTLDGSVSISG"
BASE COUNT          509 a          440 c          322 g          574 t
ORIGIN      
        1 cctgatatac tgacagagcc atcaagcgtt aaaatatcat tgcctgccag tttggggatt
       61 tccacactgg tattaccggt tatttttcca tgatatatat atgataaagg agcgctttcc
      121 tgcattttca tctgagtata taacccaatt gctttttctt tatccttacc cagcctttgc
      181 cattcgctct gattattcag cgcgccaaac aaagcgctgc catatcggtt acttttcaac
      241 agataataat ttccgtcaaa ggtcgtataa tacagatcac ctggtttgcc tgtccccccc
      301 attatattta tattattaac aaccacattt atttcttctg ctttcagagg agataacgaa
      361 agtgtgacta tggatttatc tgtctgatta tcgttgctga tttcagcacc ataatctgca
      421 gcctgaagac gggaaaaggt aaaatcatac ccatttaaat ccagtttccc accccgatat
      481 ccccagctga tattatctgc atctacctgg tttgccccgt taagtttaac tgttgcccga
      541 ccactggcta tattgatatg gctaaacgcc tgttgtttct ggtttgcatc tggctgttgc
      601 tcaagaacaa caacgccgtc cccaacttta aggccacctt tattctcacc atgaccatta
      661 acagttaatg tcccggaacc aactttatgc agattatcac cggcaatgcc attaactccc
      721 catttgacat ttgcctcgtc accgacaata attccggcac cagtccatgt actgttatta
      781 tttgtcagta cagtgtagtc tttatcaaac tgaagatatc cggctccctg ctccacactg
      841 gataccagtt cgattttccc ttcattacct gagaacacaa gattgttccc ggtatttgca
      901 tttccgttta cactaccagt catggaaaaa atcttctctc cctgactgag agtacctgta
      961 cctctcgaac gatcaaattc ccagcgtaag atattctcct ccggagaagc aacaataatt
     1021 ggtttaaggt catcctgtaa tttttgactg ataaagtcat gaggtatcag gagccagtca
     1081 tttcgtgcac cttttactcc ataattatgc agagtaactc cagctaatac ccattttttg
     1141 tcaatgttat cccaggcata taacggcgaa ccactgtctc cactctgtcc ataactggca
     1201 agaggtccct ggtttttatc aaaggtattt cccgggtttg ctgaaatcat cttcataccg
     1261 ttatagtgag tataataagg tttccccact gttccgccgg tcagataagc cttttctgga
     1321 gtgtaagcct gcagtagttt atctgtgcca tttgcataac ggatatattg agagccaacg
     1381 cccgcacggt aaaaagccgt ataccggtta ttattaaatt tatcctttcc ctgtatatct
     1441 gcgggaatta cttctgtaac aagcttattt aatcgtgggg cagaaaaatc tataccagca
     1501 aggttattat caactatttt atatatctgg tcataaccaa aagttgctga tgaaacactt
     1561 tgtaaaccac catgtttagc cgatacaaca taaccaggat gaatcaatgt ggtatgacca
     1621 tcacgagaaa gactggaaaa atcaagcatg ggaacatcaa gaaccccaac tttatttcca
     1681 ttgttgtcgt ttatttcaat tcctgtcact ccgggggtaa acacaccttt attttcagca
     1741 aagtcacgaa aagtctgata tggtatttct gcgctgaccg ttgatgcttt acccggtaaa
     1801 gcagaaagag tcactgataa taccagggaa accgtcatta atctt
//
LOCUS       DAZGSP010000275         1814 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZGSP010000275 DAZGSP010000000
VERSION     DAZGSP010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.279"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            198..1361
                     /locus_tag="ACHK74_005287"
     CDS             198..1361
                     /locus_tag="ACHK74_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFP0223919.1"
                     /translation="MEARAEATSARSRVEQARSAHWPVVTATGSKLLSQSHRYDYDYD
                     SEDILPGIRGEMNLFASGAIEADVSRSESEAEYYRYKVDEAGEETIRSFVSLYLDALR
                     EKQSIAVLEQSLSRHNAILNDLNTISIHDTGRESEFVQAEARRLMVRQQINTRTRVLK
                     TTLGKLSTWTTNPVTEADLENPFSRMTESRLLTDFTQTPQKGNPSWLASQADVESKKA
                     ALKAQELARYPRVDLTGSVTRDDRQIGVSLSWDLFNRNASYGVSEKAAQIAAATGRLD
                     SVARMIDETGRLSLITVRQSRMEMETLRRQEQASARVVDFYRLQFRVARKTLIELLNA
                     ESELYSVGLSRVQTEDQMLHGMLDYLYSQGMLLKWSGVNLSGAEEKVTGQKDR"
     gene            1366..>1814
                     /locus_tag="ACHK74_005288"
     CDS             1366..>1814
                     /locus_tag="ACHK74_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFP0223920.1"
                     /translation="MKSIFEGLSQVTALLGTPVSAETLAAGTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRRAPEDIPSLAVPVLLLLNAQEAIVVVRIEGAGLARQYHVLQPGGLR
                     KTLSHDELMAVYSGYCWFVQPQATQDRRSELPEYDMTGAWFWRVLWR"
BASE COUNT          429 a          424 c          496 g          465 t
ORIGIN      
        1 ttccgatgtc tctgcatttc aattttactc aatattttac ccggttgcat catgatgcct
       61 gattatattt cccgcgtgtc ccggaggttc gggtgaagag aatattatct ttctgttttc
      121 ttttctctgt ccacaacgtg ttcgcttctc cactgccggc cctcctgagg gatgctctca
      181 ttcatgatcc atcagtgatg gaggcaaggg cagaagccac ctctgcccgt tcccgtgtgg
      241 agcaggctcg ttcagcacac tggccggtcg tcacagcaac aggcagtaaa ctcctttccc
      301 agagtcaccg ctacgactat gactacgact ctgaagatat tttgcccggt atccgcggtg
      361 aaatgaatct gtttgcttcg ggagctattg aggcggatgt cagccggagt gagtcggaag
      421 ccgagtatta ccgttataaa gttgatgagg ctggcgagga aacaattcgc tcctttgttt
      481 cattatatct tgatgcgcta agggaaaagc aatccattgc ggtacttgaa cagagtctct
      541 cccggcataa cgcaattctt aatgatctta acaccatcag tattcacgat accgggcggg
      601 aatctgaatt tgttcaggcc gaagcccgca ggctgatggt ccggcagcag ataaataccc
      661 gcacccgagt gctcaaaacc acgctgggaa aactttcgac ctggaccaca aatccggtaa
      721 ccgaagccga tcttgaaaat cctttttccc ggatgacaga gtccagattg ttaactgatt
      781 ttacgcagac gcctcagaaa gggaatccgt cgtggcttgc cagtcaggct gatgtagaga
      841 gtaaaaaagc ggcgctgaaa gcgcaggagc ttgcccggta tccccgggtg gatttaacgg
      901 gatcagtgac ccgggatgac aggcagatag gtgtcagtct gtcctgggac ctgttcaacc
      961 gtaatgccag ttatggtgtc agtgaaaagg ccgcgcagat agctgcagct accggacgac
     1021 tggactctgt tgcccgaatg attgatgaaa ccggacgatt atcgctgata acggtcagac
     1081 aaagtcggat ggaaatggaa acgctcagac gtcaggagca ggcctctgcc agagttgtgg
     1141 acttttatcg tcttcagttc cgggtggcaa gaaaaacact gattgaatta ctgaatgcag
     1201 aaagcgaact gtacagtgtc gggctttccc gggttcagac ggaggatcag atgctccacg
     1261 gaatgctgga ttatctgtat tcccagggaa tgctcctgaa atggagcgga gtgaatcttt
     1321 ctggtgcaga agaaaaagta acagggcaga aggacagata aataagtgaa gagtattttt
     1381 gaaggattat cacaggtaac ggcattactg ggtacacctg tatctgctga gacgctggct
     1441 gccggtactg tccggacaga tgtctccggg attgattttc gttctgttgg cgagtttctt
     1501 cgcagtgaag ggtttgataa tcatctttcc cggcgggctc cggaagatat tccttctctt
     1561 gcggttcctg tgcttctttt actgaatgca caggaagcca ttgtggtggt gcgtattgag
     1621 ggtgccggcc tggcccggca gtatcatgta ctccagcccg ggggactccg taagacactt
     1681 tctcacgatg aattaatggc tgtttattca ggttactgct ggtttgttca gccacaggca
     1741 acacaggatc gccgttcgga actgccggag tatgacatga ccggggcatg gttctggcgg
     1801 gtactgtggc ggtt
//
LOCUS       DAZGSP010000276         1802 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZGSP010000276 DAZGSP010000000
VERSION     DAZGSP010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.280"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..579)
                     /gene="tssG"
                     /locus_tag="ACHK74_005289"
     CDS             complement(<1..579)
                     /gene="tssG"
                     /locus_tag="ACHK74_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP0223921.1"
                     /translation="MHPVERKSQSAPARLITRYRKQLPYINFYRFCQLLEQSQPDQPP
                     IGSGWQARQEAVRFCPYPGMGFPASEIKDAVIPEESHLPPTVHVTFMGLYGVTSPLPA
                     HYISDIAQQREGHEAAADFLDIFSHRLITQYYRIWRKYSYPATFEAGGQDKTSQYLLG
                     LARLGIPGCAQNIATPVSRFLALLPLMLLPGRT"
     gene            complement(534..>1802)
                     /gene="tssF"
                     /locus_tag="ACHK74_005290"
     CDS             complement(534..>1802)
                     /gene="tssF"
                     /locus_tag="ACHK74_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP0223922.1"
                     /translation="ADWSQVDLHRLSLYLAAEAPVSSTLHLMMTKRLAALYLRLPGND
                     ERIRIDGWFSPGGFAEEDRLWPKGDSAFSGYQLLLEYFTFREKFMFVHLNGLENISLP
                     AGISGFDIEVVLSQSWPADLPVTDDALCLHCVPVINLFTLEADPLIINGLESEYLLRP
                     KRLQDGYTEIYSVDAVAGSGRTGSAEYVPFTSFRHRGGMLRHDAPERYYHTRVKRGVT
                     GMYDTWLILGGQRWEVDRMPERETLSLRITGTNGQLPRRALQSTLLDRCEQVLQTPVS
                     VCNLCKPTLPVYPPTEDRFHWRVMSHLGTGFLNMLSSAEVLRGTLALYNWRDDELNHR
                     RLDAILAVQHHRIQRFEKGFLLRGLDVEVTLDGNGFAGEGDIHLFGEMLNRFLALYAD
                     MNQFNQLTLIVQPEGKCIRWKENHNPRLPG"
BASE COUNT          472 a          515 c          428 g          387 t
ORIGIN      
        1 tgttcttccg ggtaacaaca tcaggggcaa taacgccaga aaacgcgata ccggcgtggc
       61 aatattctgt gcacatccgg ggatccccag tctggccaga ccgagcagat actgggatgt
      121 tttatcctgc ccccccgcct caaaggtggc cgggtacgaa tatttgcgcc agatacggta
      181 atactgggtg atcagacgat gactgaatat atcgagaaaa tccgctgcgg cttcatggcc
      241 ttcccgctgc tgtgcaatat cactgatata gtgagccggc aacggagacg tgaccccgta
      301 aagtcccata aacgtcacat gtacagtggg tggcagatgg gattcttcag gaattacagc
      361 atcttttatt tcactcgcag gaaaccccat tcccgggtat ggacaaaagc gaaccgcttc
      421 ctgacgggcc tgccagccac tgccaatggg aggctgatct ggctggcttt gttccagtaa
      481 ctgacagaag cggtaaaaat taatatacgg aagctgtttc cggtaacggg taattaaccg
      541 ggcaggcgcg gattgtgatt ttctttccac cggatgcatt ttccttcagg ctgaacaatc
      601 agtgtgagct gattgaactg attcatatcg gcgtacaggg ccaggaaacg gttgagcatt
      661 tcgccaaaca ggtgaatatc tccctctccg gcaaacccgt tgccatcaag agttacctcc
      721 acatcaagcc cccggagcag aaaacccttt tcaaaacgct gaatacgatg atgctgtaca
      781 gccagaatgg catcaagccg acggtgatta agttcatcgt cccgccagtt ataaagcgcc
      841 agcgtgccac gcagcacttc cgcactgctc agcatgttaa gaaatcctgt ccccagatga
      901 ctcatcaccc gccagtgaaa acgatcttct gtcggcggat aaaccggcag cgtgggttta
      961 caaagattac atacactgac cggggtctgc agtacctgtt cacaccgatc aagcagcgta
     1021 ctctgcaacg cacgacgggg aagctgacca ttggtacctg taatacgtaa tgaaagcgtt
     1081 tcccgctccg gcatccgatc cacctcccac cgctgaccgc caaggataag ccatgtgtca
     1141 tacatccccg tgacaccccg cttcacccgg gtatgataat aacgttcggg tgcatcatgg
     1201 cgcagcattc ccccacgatg acggaagctg gtaaaaggca cgtactctgc actgccggtt
     1261 cgcccggagc ccgccaccgc atccactgaa tatatctccg tatatccatc ctgcagacgc
     1321 ttcgggcgca gcagatattc gctctccagc ccattgatga tgagtggatc ggcttcaagc
     1381 gtaaaaaggt taatcaccgg cacacaatgc agacacaggg catcatctgt cacaggtaaa
     1441 tctgccggcc aggactgact cagcaccacc tcaatatcaa atccggaaat acctgccggc
     1501 aaagaaatat tctccagtcc gttcagatga acaaacataa atttttcccg aaaggtgaaa
     1561 tattccagca ataactgata cccactgaaa gcactgtcac ctttcggcca cagccgatct
     1621 tcttctgcaa aaccaccggg tgaaaaccat ccgtcaatgc ggatccgctc atcatttccc
     1681 ggcagcctca gatacagcgc agccagacgc tttgtcatca tcagatgaag ggtgctgctg
     1741 accggggctt ctgctgccag atacaggctc agacgatgca aatcgacctg agaccagtca
     1801 gc
//
LOCUS       DAZGSP010000277         1778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZGSP010000277 DAZGSP010000000
VERSION     DAZGSP010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.281"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..183)
                     /locus_tag="ACHK74_005291"
     CDS             complement(<1..183)
                     /locus_tag="ACHK74_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFP0223923.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYQPCVNISAEPE
                     AYFRITPEDWLQAQMQG"
     gene            complement(194..892)
                     /locus_tag="ACHK74_005292"
     CDS             complement(194..892)
                     /locus_tag="ACHK74_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0223924.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMA
                     VNFGGFLSINKLSQ"
     gene            complement(892..1221)
                     /locus_tag="ACHK74_005293"
     CDS             complement(892..1221)
                     /locus_tag="ACHK74_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0223925.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(1218..>1778)
                     /locus_tag="ACHK74_005294"
     CDS             complement(1218..>1778)
                     /locus_tag="ACHK74_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0223926.1"
                     /translation="AMLTGSEQNWRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGA
                     FGGGASASSGTAIEAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVG
                     NLYRLMRGYAEGGYVGGAGSPAQMRRAEGINFNQNNHVVIQNDGINGQAGPQLMKAVY
                     DMARKGAQDELRLQLRDGGMLSGSGR"
BASE COUNT          369 a          643 c          404 g          362 t
ORIGIN      
        1 cccctgcatc tgtgcctgca gccagtcttc cggtgtaata cgaaaatacg cctccggctc
       61 tgcggagata ttcacgcagg gctggtaccg ttcgccctcc ggggtgctta tcacgaagcc
      121 gcacgactcc gctggcgcac accgccgggc atgcgccaga atcgctgatt cagtctgtgt
      181 cataaaccgg gatttactgc gaaagtttat tgatggaaag gaaaccgcca aaattgaccg
      241 ccatgccgcg catctcacac ccgcgcatgc atttactgca tctgtccttc cggatatccg
      301 tggtagggtt gtcgaactca tccgccactg ccggaccgtt atacccgcac tcatcgcccc
      361 ggtaatccca catacaggtg ttcgccagca tgatgcgacc gggaaacagc gcaccgtccg
      421 tctccgtcgg tgtcgccagc acaaacgagg ccgtcatggc cgtcagcgat gacatctgct
      481 ccaccaccca ccggtccgtc agctcctgct ccgggtctgc ctcaggattg cctgccacaa
      541 aattcaccgc atccagaaaa cgcgcataca cccggcgacg gaccaccgtg gcacccacca
      601 ggctctgcaa atcctccgcc atcccggtga ccagcccgaa aagattggac actgtcagcg
      661 acgggcgggc actgctgccc tttccgttca tctcaaagcc gctgccctca atcgggtacg
      721 cctgatattc acgcccctgc caggtcactg gctccccttt ttcattcagc tcattgcaga
      781 aaaaataccg ctcaccgccc tgcaccgtca ggtcgatttc ccagagcacc acccgcggtg
      841 actgctctga cttaaccgac tcgttaagac tttcttcgtg aatatcctgc atcagttcac
      901 cacctgctta aactccgcgc tgaactcaac gcgcaacatc ccgacccgcg cagaccaccc
      961 ggcacaggtc acctttatct gccggtatgc atagggtggc ttccacaaaa atgccttcca
     1021 gcccccgtgc tctgccagga acgcttccag atgccgggcc tcctcccggg tcacggaaag
     1081 cgtcaccctg tatgttttca ggtcagcatt cagccctgcc gccatacgct gtgagtaccc
     1141 gtcaccaaaa cgcacttcac gcaccgacgg ctgcgagttc acctccatat ccggcttcac
     1201 tttccagcga aaggttttca tcgcccgctc cctgataaca taccgccatc acgcaactgc
     1261 agccggagtt catcctgcgc ccccttgcgg gccatgtcat acaccgcctt catcagctgc
     1321 ggccccgcct gtccgttgat accgtcgttc tgaatcacca cgtgattgtt ctgattaaaa
     1381 ttaatacctt ccgcccgccg catctgcgcc ggacttccgg caccacccac ataaccacct
     1441 tccgcatagc cgcgcatcag gcggtaaaga ttccccacac ctatccggct ggttgcctcc
     1501 ttcgtgaaga caaactcccc gcggtgaact atccccgcag gctcatattt gccgcccgtc
     1561 cccgtaaatc ctccggtcgc gaaatggaag ttcgccgccg cagcctcaat ggccgtcccc
     1621 gaggaagcag atgcaccacc accgaaagca ccgccaatgg cgctgccgat actcccgaca
     1681 atccccacca ttgcctgctt aagcagaatt tctgtcatca tggacagcac ggaacgggtg
     1741 aagctgcgcc agttctgctc actgccggtc agcatcgc
//
LOCUS       DAZGSP010000278         1774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZGSP010000278 DAZGSP010000000
VERSION     DAZGSP010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.282"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            240..674
                     /locus_tag="ACHK74_005295"
     CDS             240..674
                     /locus_tag="ACHK74_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFP0223927.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            767..1033
                     /locus_tag="ACHK74_005296"
     CDS             767..1033
                     /locus_tag="ACHK74_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223928.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            1098..>1774
                     /locus_tag="ACHK74_005297"
     CDS             1098..>1774
                     /locus_tag="ACHK74_005297"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFP0223929.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFEDPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDTVSP"
BASE COUNT          400 a          506 c          507 g          361 t
ORIGIN      
        1 aacacgtgtc agccgtccag gggggagatg taccagccac aaaacttttt gccgcaggcg
       61 tcaaaaactt ttgcgtctgc ccccggacaa ctgccccgtg ccgtggctgt cgctgcccca
      121 tcggaaaggt ccactgcggg gagaaacccc gaagaagtgg acggcagaag gggctttcgc
      181 ccctccgggg gagcaaccgg tcggcccggc gacctgattc agcagaagga gacgttttca
      241 tgacagtcag cagcacgatt tccgtttttt gccgcgacgg ggtgttccgt accgtttact
      301 gccacctgca cggtgagccg acctggaacg gtcgcatcct gcatacccac tatgccaccg
      361 gtcagcaggc cgaagccctg gttgaacacg gtgatatccg ttgcctcggt ccccgttgcg
      421 acaaacccgc cgggcatacg cttcagaacc cggtggatgg tgtgacggct tattacggac
      481 gtgacagtgg tttccggatg gacagtgagg cgcgtgagta ccgttctttc atggaggcta
      541 ttgccacgga aagcacagaa gaggtgcgct tccattatgt gttcatcgac ggctactgga
      601 aggtgatgta ccgcacgccg gaaggctgga agatgaaagc gctcgcgctg gcactgcgtc
      661 gctgtccgga atgaaaaaaa gcagggcgat aaaccctgcc tttctccgcc ggcgcttccc
      721 cgtacggaag tccggcatct cattcactaa ctatggagat tatgccatga gaccatcaat
      781 tatcttcgca accgccgagt atgtaaagcg tctgcgtgaa gagtgcctgc gggagaacaa
      841 acccctgcac cgccataccc gcttcagacg ccaggaactg gcaccggatg agattaaccc
      901 ggacgtcctg gcgatgggcg gtcatatcgc cagacgctgc agtgagcaga agcgggtacg
      961 tatccccgcc atgaaagtca gcgaatgggg ccacctgctc cgcgcgcttg aaattgagcg
     1021 ggtctgccac tgattaaccc ttgccgtctg cctgtgcagg cggcaggata cgttcagaca
     1081 tctcaccagg aggaaccgtg agtaagaaga aaaacaccac aacgcccacg ccgcatgatg
     1141 ccgcgttccg gtcgttcctg gcgaatcccg acgtcgccag agattttctg gaactgcatc
     1201 ttccggcgga gtaccggcag ttgtgcgacc tgtccacgct gaagctggaa cccgccacct
     1261 ttgttgagcc ggacctgcat cagtacgcca gcgatatcct ctggagtgtg aaaaccaccg
     1321 ggggggaaga cggctatgtt tatacgctca tcgagcacca gagcaccgaa aatctgtaca
     1381 tgcctttccg catgttacgt tacagtgtgg cggcgatgca gagacatctg gagcagcaca
     1441 aaacgttgcc actggtcatt ccggtgctgt tctatcacgg tgagcgcagc ccgtacccgt
     1501 acagcatgaa ctggctggac tgttttgagg atccggcact tgcggctaaa atatacacaa
     1561 agccgtttcc gctggttgat atcactgtcg ttgatgacaa tgaaatcatg aaccatcgcc
     1621 ggatggccgc actgacgctg ctgatgaagc atatccgcca tcgtgacatg atggagctgc
     1681 tggacaaact cccgcaggtc atggtggaaa tttcagacga gcaggtgcgt gttctgattc
     1741 attacatcgt taacgcaggg gacactgtat cacc
//
LOCUS       DAZGSP010000279         1773 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZGSP010000279 DAZGSP010000000
VERSION     DAZGSP010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.283"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..340)
                     /locus_tag="ACHK74_005298"
     CDS             complement(<1..340)
                     /locus_tag="ACHK74_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223930.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSDMRAHILRR
                     DNNYQFIVTIN"
     gene            complement(602..1773)
                     /locus_tag="ACHK74_005299"
                     /pseudo
     CDS             complement(602..1773)
                     /locus_tag="ACHK74_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
BASE COUNT          547 a          343 c          276 g          607 t
ORIGIN      
        1 tattaattgt cacaataaac tgataattat tatccctacg tagaatatga gcgcgcatgt
       61 ccgatagcat actatatttt tctgaatgat tcgtttgtat ggtaaatgat attttgtcat
      121 ctcgaataga ttgttggaaa cgttctattt gccaatcatc aattttttcc acaacttcac
      181 cgagcattgt cctggcaata cttgatatat catcacctat accatttgca atatacccaa
      241 acagttcttt agtgaagtta gtaacatgct cttgcaccat atcatttgtt gcgattgtcg
      301 ttgtactgtc gttagtgcct gccacagttc caattttcat cttttacctc atataattca
      361 ccgcgaggaa tgcatagtta acatttaatc ttatgacata cacctcatgt tcgatggaaa
      421 aataattata ttttgcacaa atcattcagc cttaaagcat aatatgaaat ttcttatctc
      481 atagcagaga aagtccctaa agattgtaga ggcgtcatca gaacatgcct ctacaatcga
      541 ttaagaaaac ctatgaaata ctggcaggat taaaaagata taccacagca ctcatggagt
      601 tttaatgtgg ttgaaaacaa ctaagccctg ggacaccttt tttatcacca cgttcgttag
      661 gagctatctt ccaggaattt agccccatgg tataattatc aattttttga tgctgatcta
      721 cccctaatcc ataatggcca tatttctgtt tagttttttt gagcgtctgt ccacatttga
      781 agatatcttc aggttttggt ctgaaagcag tagtgttatt aaaataattg tcatatacag
      841 gtgtggatgc tatagcattg cttctaatta gatggataaa acgatcatcc atgtgttctt
      901 tatctttttt attaaaaata ttggctaatt cctcaggatc agtaggattt ttcatctgat
      961 taaatggaac ctgcaaagca ccgagacaat aacatttttt tcttcatctc ttattattcc
     1021 gtgatgactg gttaaaactt gcctttctga gtcataatta caaacatcga gcgttttaat
     1081 taagttatac gtatcaccgg cagagagttt atgatttgct agtcgtgcag tatagttacc
     1141 attaaaattg atttcatgat ttccagccag aacgacgaca tttttattaa tacggctatc
     1201 tttattaccc tccatatttc gcatagaatt tagcaaagta aggatatatt tatcaccaaa
     1261 aatagtacta aatctatcgc ctgtatgatc cccaaggaag atacaagggg ttatcgcatc
     1321 gtcctttaaa aaaacatgtt gatgcaactc attcaagatc atgtctctcg tatttaataa
     1381 atcgtagagc actttattat ttttatgttt gtatgcagat gaagccatct cttcgtgttt
     1441 taataattca cataataaac tccaaccctt ttccctgatg cctatatgcc ctgaaacgag
     1501 caatgcataa agtacagcgc ctactcttcc atctgtatcg ccaaaataag ccgtgttggc
     1561 attttgtggg ttaatcacac cacactgttt atagaagtct atttttgaaa acacaggttt
     1621 aacatagcta tcaaactttt ccttagtaaa cgtctcttta gagacatccc aacgccagtc
     1681 aggtttgaaa ttaccactaa gaagagtaaa gaaatttctc gctatgatag aaagcccatc
     1741 aactttataa attatatctg atttatctcc aga
//
LOCUS       DAZGSP010000280         1765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZGSP010000280 DAZGSP010000000
VERSION     DAZGSP010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.284"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            93..1217
                     /gene="gmd"
                     /locus_tag="ACHK74_005300"
     CDS             93..1217
                     /gene="gmd"
                     /locus_tag="ACHK74_005300"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFP0223931.1"
                     /translation="MDKNKIALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTE
                     RVDHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPE
                     YTADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYA
                     VAKLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCL
                     YLGNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAEQVGIKL
                     RFEGVGVNEKGIVVSVTGKDAPAVKSGDIIVKVDPRYFRPAEVETLLGDPTKAHEKLG
                     WKPEITLREMVSEMVANDLEAAKKHSLLKSHGFNVSLSQE"
     gene            1221..>1765
                     /gene="fcl"
                     /locus_tag="ACHK74_005301"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             1221..>1765
                     /gene="fcl"
                     /locus_tag="ACHK74_005301"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFP0223932.1"
                     /translation="MFLQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIP"
BASE COUNT          460 a          450 c          445 g          410 t
ORIGIN      
        1 tggcagtagt taaggatttt gagttatcag aacgtacaaa gatattagaa tcaatttaca
       61 ctaataatta ttttttataa gtgaggttat ttatggacaa aaataaaatt gcactcatca
      121 ccggtgtaac cggacaagat ggttcttacc tggcagagtt tctgctggaa aaaggttacg
      181 aggtgcatgg tattaagcgt cgcgcatcgt cattcaacac cgagcgcgtg gatcacattt
      241 atcaggatcc gcacacctgc aacccgaaat tccatctgca ttatggcgac ctgagtgata
      301 cctctaacct gacgcgcatt ttgcgtgaag tacagccgga tgaagtgtac aacctgggcg
      361 caatgagcca cgttgcggtc tcttttgagt caccagaata taccgctgac gtcgacgcga
      421 tgggtacgct gcgcctgctg gaggcgatcc gcttcctcgg tctggaaaag aaaacccgtt
      481 tctatcaggc ttccacctct gaactgtatg gtctggtgca ggaaattccg cagaaagaaa
      541 ccacgccgtt ctacccgcga tctccgtatg cggtcgccaa actgtacgcc tactggatca
      601 ccgttaacta ccgcgaatcc tacggcatgt acgcttgtaa cggtattctc ttcaaccatg
      661 aatccccgcg ccgcggtgaa actttcgtta cccgcaaaat cactcgcgca atcgccaaca
      721 tcgcccaggg gctggagtcg tgcctgtacc tcggcaatat ggattccctg cgtgactggg
      781 gccatgccaa agactacgta aaaatgcagt ggatgatgct gcaacaggaa cagccggaag
      841 atttcgttat tgccaccggc gttcagtact ccgtgcgtca gttcgtggaa atggcagcag
      901 agcaagtcgg aatcaaattg cgctttgaag gtgtcggcgt aaatgagaag ggaatcgttg
      961 tttctgttac tggtaaggat gccccagccg ttaaatccgg agatataatt gttaaggttg
     1021 acccgcgcta tttccgtccg gcagaagtag aaacgctgct cggcgacccg accaaagcgc
     1081 acgaaaaact gggctggaaa ccggaaatca ccctcagaga gatggtgtct gaaatggtgg
     1141 ctaatgacct cgaagcggcg aaaaaacact ctctgctgaa atctcatgga tttaatgtta
     1201 gtttatctca ggaataacta atgtttttac aaagaatttt tattgctggc catcgcggga
     1261 tggtcggttc cgccatcagg cggcagctcg aacagcgcgg tgatgtggaa ctggtattac
     1321 gcacccgcga cgaactgaac ctgctggaca gccgcgccgt gcatgatttc tttgccagcg
     1381 aacgtattga ccaggtctat ctggcggcgg cgaaagtggg cggcattgtt gccaacaaca
     1441 cgtatccggc ggatttcatc taccagaaca tgatgattga gagcaacatc attcacgccg
     1501 cgcatcagaa cgacgtgaac aaactgctgt ttctcggatc gtcctgtatc tacccgaaac
     1561 tggcaaaaca gccgatggca gaaagcgagt tattgcaggg cacgctggag ccgaccaacg
     1621 agccttatgc cattgccaaa atcgccggga tcaaactgtg cgaatcttac aaccgccagt
     1681 acggacgcga ttaccgctca gtcatgccga ccaacctgta tggcccgcat gacaacttcc
     1741 acccgagtaa ttcgcatgtg atccc
//
LOCUS       DAZGSP010000281         1739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZGSP010000281 DAZGSP010000000
VERSION     DAZGSP010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.285"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1038
                     /locus_tag="ACHK74_005302"
     CDS             <1..1038
                     /locus_tag="ACHK74_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP0223933.1"
                     /translation="PLIRVETATGEQAKTQRRSLADTLQQSGGEDGGSVVFPPVLVQM
                     LDRLESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLA
                     LVSTEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQ
                     GRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPS
                     KLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDSQ
                     YPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAK
                     MKQRTSRGIRK"
     gene            1035..1604
                     /gene="ybfC"
                     /locus_tag="ACHK74_005303"
     CDS             1035..1604
                     /gene="ybfC"
                     /locus_tag="ACHK74_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFP0223934.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIKIQHELIRSEEK"
BASE COUNT          535 a          364 c          424 g          416 t
ORIGIN      
        1 ccactcatca gggttgaaac cgccaccggt gagcaggcga aaacgcagcg ccgcagccta
       61 gcggataccc ttcagcagtc cggcggcgaa gacggtggca gtgtggtgtt cccgccggtg
      121 ctggtgcaga tgctcgaccg gctggaaagt gaaatcctgg ctgaccgggt gagtgaggaa
      181 agccgccgct ggctggcatc gtgcggcctg acggtggagc agatgcaaaa ccagatggac
      241 ccggtgtaca cgccggcgcg aaaaatccac ctgtaccact gcgaccatcg cggcctgccg
      301 ctggcgcttg tcagcacgga aggggcaaca gaatggtgcg cagaatacga tgaatggggc
      361 aacctgctga atgaagagaa cccgcatcag ctgcagcagc ttatccgcct gccggggcag
      421 cagtatgatg aggagtccgg cctgtattac aaccgccacc gctattatga cccgctgcag
      481 gggaggtata tcactcagga tccgattggg ctgaaggggg gatggaattt ttatcagtat
      541 ccgctgaatc cggttcagta tatagattca atgggactgg catcaaaata tggacactta
      601 aataatggcg gatatggagc gagacccaac aaaccgccta cgcccgatcc aagtaaattg
      661 ccggacatag cgaaacaatt aagactgcca tatcctattg accaggccag tagtgcgcct
      721 aatgttttca aaacattctt cagagcatta agcccttacg actacacact gtattgcagg
      781 aagtgggtaa aaccaaatct gacttgtacg ccacaggatg attcccagta tccagggatg
      841 gatacaaaga cagcaagtga ttacctgcca cagacaaatt ggccaacaac tcaattacca
      901 ccaggatata cttgtgcaga accctattta ttcccagaca ttaataaacc cgatgggcca
      961 gcaacagcag ggatagatga tttgggtgaa attttagcta agatgaaaca gagaacatcg
     1021 agaggaataa gaaaatgaaa agagttttgt tctttttgct gatgatattt gttagttttg
     1081 gtgttatagc tgattgcgaa atacaagcta aagatcatga ttgttttact attttcgcta
     1141 agggtactat tttttccgcg tttcctgtct taaataataa agccatgtgg agatggtatc
     1201 agaatgagga catcggtgag tattattggc aaacagaact gggtacatgt aaaaataata
     1261 aattcacacc aagcggggcg agactcttaa ttcgcgtcgg gtcactacgc cttaatgaaa
     1321 atcatgccat caaaggaacg ttgcaggagt tgataaatac agcagaaaaa acggcgtttc
     1381 tcggcgatcg gtttagaagt tatataagag cgggtatcta tcaaaaaaag agcagtgatc
     1441 cagttcagtt gttggcagtc cttgataatt caatcatggt aaaatatttt aaagatgaga
     1501 aaccaactta tgctcgaatg acagcacatc tcccgaacaa aaatgagtct tacgaatgtt
     1561 taataaagat acagcatgaa ctaattcgta gtgaagagaa ataaaatctc ttcctgagaa
     1621 aacaaattaa tcattgtgaa aaattatata actcatggaa taggtgttta ttttgcgttc
     1681 catttgcagg gaaagatcac gtaacgctac ttttttgtac taaataattc gaattttaa
//
LOCUS       DAZGSP010000282         1731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZGSP010000282 DAZGSP010000000
VERSION     DAZGSP010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.286"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            165..380
                     /gene="essD"
                     /locus_tag="ACHK74_005304"
     CDS             165..380
                     /gene="essD"
                     /locus_tag="ACHK74_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFP0223935.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            385..696
                     /locus_tag="ACHK74_005305"
     CDS             385..696
                     /locus_tag="ACHK74_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFP0223936.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     I"
     gene            693..1226
                     /locus_tag="ACHK74_005306"
     CDS             693..1226
                     /locus_tag="ACHK74_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0223937.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            1223..1721
                     /locus_tag="ACHK74_005307"
                     /pseudo
     CDS             1223..1721
                     /locus_tag="ACHK74_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
BASE COUNT          496 a          350 c          441 g          444 t
ORIGIN      
        1 gtattattat tttatatatg actttctttc aaaataatta ctcacatttt taatgtgtat
       61 gtttctttag cgccgttgag aacaacgtgt gctgtcaaaa ctaccccgta gactccgatc
      121 tttttcaaac atattgcacc atccgtgtac atcggggtga ggatatgaaa tcaatggata
      181 agttaacaac aggtgttgcc tatggcacat cggcgggtaa tgctggtttc tgggcattac
      241 agttactcga taaagtaact ccgtcacagt gggctgcaat cggtgtgctg ggtagcctgg
      301 tttttggcct gctgacgtat ctgacaaatc tttatttcaa gattaaagaa gacaggcgta
      361 aggctgcgag aggagagtaa tccaatgact caagactatg aactggttgt gaaaggagtc
      421 cgtaattttg agaataaagt tacggtaact gtagccttac aggacaagga acgctttgac
      481 ggtgaaattt ttgacctgga tgtcgccatg gaccgtgttg aaggagctgc gctggagttt
      541 tatgaggcag cagccagaag gagcgtccgg caagtcttcc tggaagtagc agaaaaattg
      601 tcagaaaaag ttgagtctta tctgcagcat cagtactcct ttaagattga aaatcctgcc
      661 aataagcacg agcgtcctca tcataaatat atatgaacac aaaaatcaga tacggcctgt
      721 cggctgccgt tctggcgctg attggtgctg gcgcatctgc tcctcagata cttgaccagt
      781 ttctggacga aaaagaaggt aaccacacaa tggcataccg cgatggttct ggcatatgga
      841 ccatctgtcg gggtgccaca gtggtggatg gaaaaaccgt ttttcccaat atgaaactgt
      901 cgaaggaaaa atgcgaccag gtcaacgcca ttgagcgtga taaggcgctg gcatgggtgg
      961 agcgcaatat taaagtacca ctgaccgaac cacaaaaagc gggtatcgcg tcattttgtc
     1021 cctataacat tggccccggt aagtgtttcc cgtcgacgtt ttataagcgg ctgaatgctg
     1081 gtgatcgtaa aggtgcatgc gaagcgattc gctggtggat taaggatggc ggacgcgatt
     1141 gccgcattcg ttcaaataac tgttacggtc aggttattcg tcgtgaccag gagagcgcat
     1201 taacctgctg ggggatagaa cagtgaatca gatattcatg gtgatttttc tcgtgttgtc
     1261 aggatttatc gtcggaaatg tctggagcga cagaggatgg caaaaaaaat gggcggaacg
     1321 tgatgctgcc gcattatcac aagaggtaaa tgctcaattt gctgctcgaa taattgaaca
     1381 ggggcgaact atagcccgtg atgaggctgt taaagatgca caacagaaat ctgctgaaat
     1441 ttctgccagg gctgcttatc tgtctgatag tgttaaccag ttgcgtgccg aagcaaaaaa
     1501 aatatgccat acgccttgac gcagcgaagc ataccgcaga tcttgccgct gccgtcagag
     1561 gcaaaacaac caaaaccgcc gaaggaatgc tcaccaacat gctcggagat attgcagcag
     1621 aagctcagct ttatgctgaa attgctgacg aacgctacat cgcaggagtg acttgtcaac
     1681 agatctatga atctttaaga gataaaaagc atcaaatgta gggtaatatt a
//
LOCUS       DAZGSP010000283         1715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZGSP010000283 DAZGSP010000000
VERSION     DAZGSP010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.287"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..576
                     /locus_tag="ACHK74_005308"
     CDS             <1..576
                     /locus_tag="ACHK74_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0223938.1"
                     /translation="AWLKEKGAFDQPYWMMKASLLAEFNKVITDVGPGKLNLGGCAIE
                     VMGTYNAAIVRVTIGEYGGDGFLNGTVCTCTVYGDTQRFHWRVDYSTKNKPTTASLTV
                     NGWERDEVTGRLRQWGSIEVSEDDGKLMTQHSIRFPVAFPVAALNAQVSAVGDPNVIK
                     MYTLSNPSLVSVTLITVRGCYGKFSWEAIGY"
     gene            586..810
                     /locus_tag="ACHK74_005309"
     CDS             586..810
                     /locus_tag="ACHK74_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0223939.1"
                     /translation="MEQYVFSPSENMFYPLSLRPVYEAAGRWPEDGIVVDYVVYKVFA
                     ADAAPAGMKRGVGAEKMPVWVPVSEEGTET"
     gene            810..1475
                     /locus_tag="ACHK74_005310"
     CDS             810..1475
                     /locus_tag="ACHK74_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFP0223940.1"
                     /translation="MHIRNFSYYTPAEPDVAGAMYLKSEDGQDWYECQALFSPETLKV
                     VYDSRGVITGYGKDTALLWPVNQSVAEVPDTPESRKIDLSGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVPVTAEELIALSEAAEQAMFTKGMEIHIRQRTMKKELEKLTSADEILAYRV
                     DWK"
BASE COUNT          458 a          343 c          542 g          372 t
ORIGIN      
        1 gcctggctga aagaaaaggg ggcatttgac cagccttact ggatgatgaa ggcatcactg
       61 cttgcggaat ttaataaggt catcacggat gtcggaccgg gaaaactcaa tctgggaggc
      121 tgcgccattg aggtgatggg gacgtataac gcagccatag tccgggtcac catcggcgaa
      181 tacggtggcg atggttttct gaacggcacg gtctgtacct gtacagttta cggagacaca
      241 caacgttttc actggcgggt ggattacagc acaaaaaaca agcccacaac agcaagcctg
      301 acagtaaatg gctgggagcg tgatgaagta accggtcgtt tgcggcaatg gggcagtatt
      361 gaggtaagtg aggatgatgg taagcttatg acacaacact ctatccgttt tcctgtcgct
      421 tttccggttg ctgctctgaa tgctcaggtg tctgcagtgg gagaccccaa cgtcattaag
      481 atgtataccc tgtcgaatcc gtcattagtg tctgtgactc tgataacagt caggggatgc
      541 tacggcaagt tttcatggga agcgatcgga tactgagggc attgtatgga acagtatgtt
      601 ttttcaccgt cagagaatat gttttatccc ctgtccctgc ggccggttta tgaggcagca
      661 gggcgctggc cggaagacgg tattgtggtg gattatgtgg tgtataaagt ctttgcagcg
      721 gatgctgcac cggcaggtat gaagcggggc gtcggtgcgg aaaaaatgcc ggtctgggtg
      781 ccggtatcgg aggagggcac ggaaacatga tgcatatcag aaatttttcg tattacaccc
      841 cggcagagcc ggatgttgca ggcgcgatgt acctgaagtc tgaggatggt caggactggt
      901 atgagtgtca ggcgttgttt tcgccggaga cgctgaaggt ggtgtatgac agccggggtg
      961 tgattacggg atacggtaag gacactgcgt tactgtggcc ggtgaaccag agtgtggcag
     1021 aagtgccgga taccccggag agccggaaaa ttgacctgag cgggcgctgg ggctttgacg
     1081 gggagaaaat cacggacctg ctgaccgcgg agaaagcgcg agggatgaag ggcgatgaaa
     1141 taaacgcgtg gcgtaatgcg atggaagcgg cgaactacac gtttgagcac aatgggcgta
     1201 aatgggacta cgggaagtca acgcagacgc gtcttgagcc gtcggtggct gcagcgaaag
     1261 cggggaaact gccggaggcg tttttctgga cggatgcgga aaacaatgat gtgccggtga
     1321 cagcagaaga gcttatagcg ctgagtgaag cggcagagca ggcgatgttc actaaaggga
     1381 tggaaatcca cattcgccag cgcaccatga agaaggagct ggaaaagctg accagtgcgg
     1441 atgagatact ggcatacagg gtggactgga aataaaaaga aggggagcgg atgctcccct
     1501 gatatctgag tagtatgctg tttatcgtta tcattcatca aaagaatatt accgtatggc
     1561 acggcagcag aacatgaaat ttgtctcaga actgcaaatc agcattacgg gtagtcaggc
     1621 agataacaca acgggataaa aaaacagcaa aatgcattga taaaaaattt ttacaggtag
     1681 gttaccgggc gttatgtgac gcatatcaaa aacag
//
LOCUS       DAZGSP010000284         1622 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZGSP010000284 DAZGSP010000000
VERSION     DAZGSP010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.288"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..282
                     /gene="tssJ"
                     /locus_tag="ACHK74_005311"
     CDS             <1..282
                     /gene="tssJ"
                     /locus_tag="ACHK74_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFP0223941.1"
                     /translation="ADYQALFTGDSEVLAGDIIAQKDVWLQPGGSVAVDMPLDDAAKF
                     TGVAAMFLEPDRKKNTWRVVLSLDELEPDTPRLIEVSGNTLTLLPVKDK"
     gene            285..734
                     /gene="tssE"
                     /locus_tag="ACHK74_005312"
     CDS             285..734
                     /gene="tssE"
                     /locus_tag="ACHK74_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP0223942.1"
                     /translation="MPRPSLYEILYGNFAGGLELNQVSAEEQVILSVLDNMQRILNTR
                     AGSLKHLPDYGLPDMTSILQGMPGTAHQLMRVLSDVLLKYEPRIKRVGVTMQEQSQPG
                     ELHYVIDAELKDAGLVRYGTTFMPEGRVLLRYLKQQQFVDNVMTFRG"
     gene            787..1092
                     /locus_tag="ACHK74_005313"
     CDS             787..1092
                     /locus_tag="ACHK74_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223943.1"
                     /translation="MNPLFNDIQMRLFYLNHAPYSWHWNVRFRPQEAVYIGNDACHIT
                     ITCNQSGFHLTRDGQRLFTERYIRTLSELLAVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            1147..>1622
                     /locus_tag="ACHK74_005314"
     CDS             1147..>1622
                     /locus_tag="ACHK74_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFP0223944.1"
                     /translation="MTSEVKLKTGNDPRSLPDYAALRDEISKLTHPARPDVDWRYVET
                     LCLRLYEHNGVELQTASWYTIARMHTTGLSGLNEGLALIVALTRHHWPVMWPLNTHAR
                     LEIITGLFNRLQKTLRAMPPDDRDNLPLLYQTETFLKALSDTLARHELKQSSKVALP"
BASE COUNT          395 a          376 c          446 g          405 t
ORIGIN      
        1 gcagattatc aggcgctttt tacgggggac agtgaagttc tggcaggtga tatcattgca
       61 cagaaagatg tatggctgca gccaggtggt tcagtggccg tggatatgcc tctggatgat
      121 gcggcgaaat ttaccggtgt ggcagcaatg tttctggagc ctgaccggaa gaaaaatacc
      181 tggcgagtgg tgctgagcct ggatgagctt gagccggaca cgccccgtct tattgaagta
      241 tccggtaaca ccctgacgct gttaccggtg aaggataaat agtgatgccg cgtccttccc
      301 tttatgaaat tctctatggc aatttcgctg gcgggctgga gttaaaccag gtcagtgcgg
      361 aagagcaggt tattctttcg gtgctcgata acatgcagcg tattctgaat acccgggccg
      421 gaagccttaa acatcttccg gattatggtc tgccggatat gacaagcatt ctgcagggaa
      481 tgccgggaac ggcgcatcaa ctgatgcgtg tgctttcaga tgttctgctg aagtatgagc
      541 cacgtattaa gcgtgtcggg gtgacgatgc aggaacagag tcagcctggt gagttgcatt
      601 atgtcattga tgcagaactt aaagatgcag gactggttcg ttacggaaca acttttatgc
      661 cggagggcag ggtgttactg cgatatctga agcaacagca gtttgtggat aatgtgatga
      721 ctttcagggg gtgatttatg gttcccctta tccgtgtgct taacagacat aaacgagggc
      781 tgaatgatga acccgttatt taatgacatc cagatgcgcc ttttttatct gaatcatgca
      841 ccgtattcct ggcactggaa tgtcagattc aggccacagg aagccgtata tattggtaat
      901 gatgcctgcc atattaccat tacctgtaat cagtccggtt ttcatttaac cagggacggg
      961 cagcgactgt tcactgaacg ttatatcaga acactcagtg agttattggc ggttctgaag
     1021 cggcggtggg atgtgacacc ggcaatcatc cgtgcggtgg aatacctttc ccgggtaccg
     1081 gtatcacact gacgctcttt ccgggaatat taattatgat ttgatacagc aacactgaat
     1141 ctgccgatga cttctgaagt aaaactgaaa acaggtaatg acccccggtc attaccggat
     1201 tatgctgccc tgcgcgatga aatcagcaag ctgactcacc cggcacgtcc ggatgtggac
     1261 tggcgttatg tggagacgct ctgcctgcgt ctgtatgaac acaatggcgt ggaattacag
     1321 acggcatcct ggtacaccat cgcccggatg catacaacag ggttaagtgg cctgaatgaa
     1381 gggctggctc tgattgtggc gctgacccgg catcactggc cagtgatgtg gccgctgaat
     1441 acccacgccc ggctggaaat tattaccggt ctgttcaacc gtctgcaaaa aacgcttcgg
     1501 gccatgcctc cggacgaccg tgacaacctg cccctgctgt atcagaccga aacattcctg
     1561 aaggcactga gtgacacact tgcccggcac gaacttaaac agagcagtaa agtggcttta
     1621 cc
//
LOCUS       DAZGSP010000285         1585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZGSP010000285 DAZGSP010000000
VERSION     DAZGSP010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.289"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..330
                     /locus_tag="ACHK74_005315"
     CDS             <1..330
                     /locus_tag="ACHK74_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0223945.1"
                     /translation="EVTAIAEKIRLLDKELRRASVSLKTLKSKGVNSFSDFYAIDLTS
                     KNGRELCRTLAYKTFEKIIINTDNKSCDIYFMNGIVFKHYPLMKVISAQQAISALKYM
                     VDGEVYF"
     gene            442..765
                     /locus_tag="ACHK74_005316"
     CDS             442..765
                     /locus_tag="ACHK74_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFP0223946.1"
                     /translation="MDAGVLVLAVQQSPITKQFTDNELCTLAWLWRAGNVMLITYQNV
                     TPLLQVAEHREAGRFTSIEQEYPQILNKAQAILARETAHVKFRPWQDDKWSRVLPHLR
                     SDRLQ"
     gene            1227..1583
                     /locus_tag="ACHK74_005317"
     CDS             1227..1583
                     /locus_tag="ACHK74_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223947.1"
                     /translation="MDTIITWMGDRLLGWITNKSDLRQRAITGLTPAICSTILYTEKL
                     KRGEPNNPNEEEKLYRLWYEASSQVVDFDRELAKRCLDKSEYWLHSELYSPEKVGELN
                     ISLVGMKATLEGIKHN"
BASE COUNT          502 a          297 c          353 g          433 t
ORIGIN      
        1 gaagttaccg ctatagcaga gaaaataaga ctattagata aggaattaag aagggcttcg
       61 gtatcattga aaactttgaa gagtaaaggt gtaaattcat tcagtgattt ttatgctatt
      121 gacttaacca gtaaaaatgg acgagagtta tgccgtacac ttgcctataa aacattcgaa
      181 aaaatcataa ttaatacgga taataaaagc tgtgatatct attttatgaa tggcattgtt
      241 tttaaacact atcctttaat gaaagtaata tccgcccagc aggcgataag tgctctcaaa
      301 tatatggttg atggtgaggt ttatttttga gtaataatca ctttttcaac cgtgctatag
      361 taagaaagtt aggtaagtac aataaaatta tctatcctga acgaagcgtc ctgagctatg
      421 gttttactat agggactgcc aatggatgct ggcgttctcg ttctagcagt tcaacaatcc
      481 ccaatcacaa aacaattcac tgataacgaa ctttgcacac tcgcctggtt atggcgagca
      541 gggaatgtga tgttaattac ctaccagaac gttactcctc ttcttcaggt tgcggagcat
      601 cgtgaagctg gtcgcttcac ttctatcgag caagaatatc cccagatact caataaggca
      661 caggcaatcc ttgccagaga aacggcacat gtaaaattcc ggccgtggca ggatgataag
      721 tggagtcgag ttttgccgca tttacgttcg gatcgattac agtagattcc aattaacaat
      781 gagaaacaca tagccacccc gtggtattga aaccatataa tgttggattt gaaaacagat
      841 ctttttccat gtattaataa ctacatcccc gcgagtgatt caaaaaggag ggcccaattt
      901 tgtccgagtt tttgtattcc cccgcatgcc gctgcggagc actacatctg agtgtctgac
      961 taggggataa aattagactg gatagtgaga agaaagtggc gtgctaggct gtgccgagtg
     1021 ctaccagtac accctggggg tgtgcagctt tcgccgagac tgtagtgggt atcggttaat
     1081 gcacgaaaaa ccgagaggtc agacaaccaa tttgccgtag gattgtttcc ggtgcgatac
     1141 cggtgtacta actgaaagca atgcgaaaaa gcataaactc ggtccttcag tcgccctaca
     1201 cactatttac taagaagggc taaagcatgg atacaattat cacatggatg ggagatcgtc
     1261 tgttggggtg gataacaaac aaatccgatc tgcggcagag ggcaatcact ggattaactc
     1321 ctgcgatttg ttcaacaatt ttatatactg aaaaattaaa acgtggtgag cctaataacc
     1381 caaacgaaga agaaaaactt tataggcttt ggtatgaagc gtcttcccaa gtcgtagact
     1441 ttgatcgaga gctggctaaa agatgtttag ataaatcaga atattggctt cattctgaat
     1501 tatatagccc tgagaaagtt ggagagctaa acatttcttt ggttggtatg aaggcaacgc
     1561 ttgaaggaat aaagcacaat taaaa
//
LOCUS       DAZGSP010000286         1554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZGSP010000286 DAZGSP010000000
VERSION     DAZGSP010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.290"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..310
                     /locus_tag="ACHK74_005318"
     CDS             <1..310
                     /locus_tag="ACHK74_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HFP0223948.1"
                     /translation="YNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHDWAN
                     YASADYFRNIYNISPWRNIPYKKAVKKHEYKEKYKHLLYQKKFLDGVFTAIKYNVMKG
                     "
     gene            319..1461
                     /locus_tag="ACHK74_005319"
     CDS             319..1461
                     /locus_tag="ACHK74_005319"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFP0223949.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          524 a          249 c          294 g          487 t
ORIGIN      
        1 atataattgt atttatacta taaagtcaga atttgaagaa aaaaatagtg aatattacac
       61 ccggtttatt aatgatgaca ctgtcttcat acattatact ggtataacta agccatggca
      121 tgattgggcg aactacgcct ctgcagatta ttttcgtaat atttataata tatcaccatg
      181 gagaaatata ccttataaaa aagctgttaa aaaacatgag tacaaagaaa aatataaaca
      241 cttgctttac cagaaaaaat ttctcgatgg tgtttttaca gcaattaaat ataatgttat
      301 gaaaggttaa tatacgagat ggttgataaa ataatattta cggttactcc tattttttca
      361 attcctcctc gcggagcggc tgctgttgaa acgtggatgt atcaggttgc tcagcgtacc
      421 aattttccaa atcgtattgt ttgtatcaaa aatccaggat actcaaatta cacctttgtt
      481 aatgataatt gtagtattca tagggttgga ttcagtcgca tatataagcg gttatttcaa
      541 aaatggacaa gacttgatcc tcttccatat tcacagagaa tattgaatat tgcacatgat
      601 tttcctataa cgaaagaaag tgttattgta gttcataata gcatgaagct atacactcaa
      661 attcgtaagc gagctcctca ggcaagagtg gttatacata tgcataatgc atttgaacca
      721 aaattgcttg agcaaaatgt taaaatgata gtgcctagtt tatacttgaa aaagtattac
      781 caatcttatt tagcaaatgc agatattgag attgtaccaa atggaattga tttagaaacc
      841 tatcaatcta attttcagcc gatcactaga agcgaactta atatctcccc tgaagagaaa
      901 attatttttt atgctgggcg tatcgtccct gacaaaggca tattgctgtt gatgcaatca
      961 ttcgaaaaat tggccgctgc tcacaaaaat ttaaaattag ttgtgattgg tgattataca
     1021 gaaatgagta aaagtgacaa aggtgcatat caacgcaatg tcagggaaat tgcaaaacgt
     1081 ttgaaggatc gttgcattat gttaggaagc ataccgcctg aaaagatgca ttgttattac
     1141 ccactggcag atttagtcgt cattccttcc cagttccagg aaccattttg catggtggca
     1201 attgaagcta tgggtgctgg taagcctgtt ttagtgagta cgcgtggggg gatgacggaa
     1261 tttgtcaaag aaaatactac gggattccat ttaaaagaac ctatgactgc agactccata
     1321 agcagcgata ttttaaaaac gttagcgaat cctgaattaa cagcagttgc aaagcaaggt
     1381 caggactttg tgtttgaaca ctacagttgg gatggcgtaa ctcaaagatt cgaagaggtt
     1441 attcataatt ggtttgaata aataaaaagg ccgcattatg cagccttttt tattttttac
     1501 ttgtttttca tcgctaataa taagccggcg taaacgccta ataaatttgg ttca
//
LOCUS       DAZGSP010000287         1548 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZGSP010000287 DAZGSP010000000
VERSION     DAZGSP010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.291"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(348..506)
                     /locus_tag="ACHK74_005320"
     CDS             complement(348..506)
                     /locus_tag="ACHK74_005320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223950.1"
                     /translation="MSAFEGLSGDSSPKEQQRDHRQHARGDPARRKSMTERSERIDEE
                     AEENRKPH"
     gene            complement(583..873)
                     /locus_tag="ACHK74_005321"
     CDS             complement(583..873)
                     /locus_tag="ACHK74_005321"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223951.1"
                     /translation="MIVYPALLFCRLKILCVFLLPVLQKKPSRHGSGSALSATGNRLG
                     RTPDPHRCNTDGLRQPTNRMAACSVQMGFAGGWRGSWRPESPWLGVSARLTS"
     gene            complement(921..1397)
                     /locus_tag="ACHK74_005322"
     CDS             complement(921..1397)
                     /locus_tag="ACHK74_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0223952.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          354 a          410 c          386 g          398 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctccatcgtg tttttgccgt ccgttcaccg ttaaaacgcc
      241 tcagaaatcg ggacaggatg tgtaatcgta taaccgcgca cgcacgccga tacgattacc
      301 acctggtcag ggcttcaccc cgacaccccg catcagtgcg taagtgctca atgtggcttc
      361 cggttctctt ccgcttcctc gtcgattcgc tcgctacgct cggtcatcga cttgcggcga
      421 gcgggatcac ctctcgcatg ttgtcggtga tcccgttgtt gttctttcgg ggatgagtcg
      481 ccagatagcc cctcaaacgc gctcacaagc tctcagacgc acgaaacggg ttttagatgc
      541 ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaggcgcgc
      601 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcccc ctgcaaagcc
      661 catttgcacg gaacaggcag ccatgcggtt ggtaggttgt cgtagaccgt cggtgttgca
      721 gcgatggggg tctggggttc tccccagacg gtttccggta gcgctcaagg cgctgccgga
      781 accgtggcgg ctcggttttt tctggagaac ggggagcaaa aacacgcaaa ggattttgag
      841 gcggcaaaag agcaatgcag gatagacaat catggtcaga acgttagcac gtagcccctg
      901 attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcggcg ctgaatatca
      961 ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt agtgttgtat
     1021 cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc aacggggatt
     1081 ctaactaatg cattttttga ttctaagtaa ttgagccaac gaataattgt gcttctgcta
     1141 actccaagtt tttcagctag gaattgctga tccgcaacga cagcgccaca cgtaggatcg
     1201 atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct atccttcagc
     1261 agctctcgaa cgcgttccca accttttggg gatacctgtg taaaccttga atttttcttt
     1321 gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt cagtcgtttt
     1381 tctgaatcaa taaacatgat ttttccctct gaaaatccca tgcactaacg ttggttcaaa
     1441 tctaagccat catgacacat ctgtgcaaca ccttgttgcg tgttgtcgca tcctggatgc
     1501 gacaacgttg tgtcaccctg gatgcgacag gtaaataaaa aacacact
//
LOCUS       DAZGSP010000288         1531 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZGSP010000288 DAZGSP010000000
VERSION     DAZGSP010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.292"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..242)
                     /locus_tag="ACHK74_005323"
     CDS             complement(<1..242)
                     /locus_tag="ACHK74_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011094339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223953.1"
                     /translation="MMKIFKLSTSQPRSPHLWQVAGLKHSTGMSLLGQIREGLDGRVA
                     QLIIDWAKISQADLCKMSGIPPKAFSRSLRTRFSAT"
BASE COUNT          462 a          337 c          299 g          433 t
ORIGIN      
        1 tgtgtggcac tgaatcgagt tctcaggctg cggctgaaag cttttggcgg aataccggac
       61 attttgcata agtctgcttg cgatatcttc gcccaatcaa tgattagttg agcaaccctg
      121 ccatccaggc cttctctaat ctgcccaaga agagacatac cagtcgagtg ttttaaccca
      181 gctacttgcc ataggtgtgg cgacctgggc tgtgatgttg acagtttaaa tattttcatc
      241 attacccgcc ttccgataat tcaggctttt caaaataaat attatattaa aagatcagtc
      301 atatatacga catgctgata tcactggcac tgattctaat atccagtatt taaacagttt
      361 ttcttaagac gaaggtactg tttacccgga acctgcagaa caattaatga aaactactgg
      421 tagaattaaa atgtaagggc aatttttatt atccaactaa tagttttttt gactaaaagg
      481 ttgattggcg gccttatccc cttctgaaag cactatcaaa gggagcaata cgatggttta
      541 ttgttgctcc tgatatgtct gacgggatcc tatggcacag gaaagagatc catgtcaaaa
      601 aacatcgaat gaactgacca catcaagcgg aggatagcat tataaccata tgattacatt
      661 caccttctac tcctccgcac accagaaatt atgccacatt tccacatctt aatttcggcc
      721 gacttaccac cattggtgtg tgattgctac gtagtatcca gtatacccca cagacttagc
      781 atgcgttgca atttaaactg gttccatgaa tctccagaca gttggtatca cttacctgag
      841 tgatagcctt aacactcgtt tttaaaccag cagctagaac ccgatatgat tgtgtcgagg
      901 gtcgcatgtc agcatgatgt gctgccagtc agctgttcct gcggcgttaa ctgaatcagg
      961 aagacagcct cactgccgtt gccgctggtg aactcgttgc gtccgcgtag gggcagtcat
     1021 cgacgatgaa gaaaatcgga gagctaaagc tcctgccggg caagaaaaac atgagaaact
     1081 ggttactgaa aaggtcgttg aatatggctg gcaaaaaaaa tagattatgc acatgccctt
     1141 attaccccgg aacggcgaat tagacttatc aggctttgtc acgagatctc acatgcacaa
     1201 ctgcatacca tggcccatcc ttcgaagaac tagtcggaac gtctgttaaa ccttcggcaa
     1261 ttcagtttta aaattgatag cagttaatcc cactaaatgg tttacccgtt taattatgtc
     1321 agcaaaaaca aaaagttact tttcatgggt atggataaag tcttaaatgc gatgttttac
     1381 attaaaaagc ggacacctcc catcgataaa ttgtctgcaa tctccacaaa ccctgctttt
     1441 caacttaaga aaatgaacat taaaagaaaa atttggaagt cccacacttt cgtaactcgc
     1501 atacacaaat tcatttttta taaaattgtt a
//
LOCUS       DAZGSP010000289         1518 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZGSP010000289 DAZGSP010000000
VERSION     DAZGSP010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.293"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1518
                     /locus_tag="ACHK74_005324"
     CDS             <1..>1518
                     /locus_tag="ACHK74_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223954.1"
                     /translation="DELRICASCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQDIY
                     ERSYTGFISDATANGLILMKLPETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNA
                     RILGDNNARNGADRLVSGTEIQTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVP
                     RDQYAGAVETMKNKIREGKVPGVTDPEEASRLIRRGHLTYTQARNITRFGTIESVTYD
                     IAEGSVVSLAAGGISFALTASVFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQL
                     HRLSVVQGMLKHIDFSTASPTVRLALQKGTGAGNISALNKVMKGSLVTSLALVAVTTG
                     PDMIKMLRGRISGTQFIRNLAVASSCVAGGAVGSVAGGILFSPLGPFGALTGRVVGGV
                     LGGIIASAVSGKIAGALVEEDRVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVI
                     DQNALEIIFAAGIQQRAATNMLIKPLVVSIIRQRPVMEYDASHLGNMVNRLEETL"
BASE COUNT          368 a          374 c          432 g          344 t
ORIGIN      
        1 tggatgagct tcgtatctgt gcctcctgcg ggctgacaga agtacatcat gccccggaaa
       61 atcataagcc ggatccggaa tggtactgct cctctctttg tcgcgaaaca gaaacactgt
      121 gtcaggacat ttatgaacgt tcttacaccg gttttatttc cgatgcaacg gcgaatggtt
      181 tgattctcat gaaactgccg gaaacctgga gtacaaatga gaaaatgttt gcttccggag
      241 ggcagggaca tgggtttgcc gctgaacggg gaaaccatat tgtcgacaga gtccgtctga
      301 aaaacgcacg gatcctcggt gataataatg ccagaaatgg agcagacaga ctggtcagcg
      361 gaacagaaat ccagacgaaa tattgttcaa ctgcagcccg tagcgtcggt gcggcattcg
      421 acggacagaa cggacagtat cgttacatgg gaaatcatgg tcccatgcaa ctggaagtcc
      481 cccgtgatca gtatgccggc gctgtggaaa ccatgaagaa taagatccgc gaaggtaaag
      541 tacccggtgt aaccgatccc gaagaagcgt cccggctgat tcgtcgggga catctgactt
      601 atacccaggc ccgtaatatc acccggttcg ggaccatcga atcggtcact tatgatattg
      661 ccgaggggtc ggttgtcagt ctggcggccg gagggatcag ttttgccctg acggcatcgg
      721 tcttctggct cagcaccggc gatcgcgatg ctgccctgca gacagctgct gtccaggcag
      781 gaaaaacctt cacccgcaca ctggctgtct acgtcacaac ccagcaactt caccggctca
      841 gtgttgttca gggtatgctg aagcatattg atttttcgac ggccagcccg actgtccgac
      901 tggctcttca gaaggggacc ggtgcaggaa atatcagtgc cctgaacaaa gtgatgaagg
      961 ggtcgctggt gacatctctg gcactggtag ctgtcacaac cggccctgac atgatcaaaa
     1021 tgttgcgggg acggatctcc ggtacgcagt tcatcaggaa tcttgccgtg gcatcttcct
     1081 gtgtggcagg tggtgctgtc gggtcagtgg cgggcgggat attgttcagt ccactgggac
     1141 catttggtgc actgacaggg cgtgtggttg gcggtgttct ggggggaata attgcctccg
     1201 ctgtatcagg aaaaattgcc ggagcgctgg ttgaagaaga tcgcgtcaaa attctggcaa
     1261 tgattcagga gcaggtgaca tggcttgccg gcagtttcct gctgaccgga catgagattg
     1321 aaaatctgaa cgcgaatctg gcccgtgtta tcgatcagaa tgctctggag atcattttcg
     1381 ccgccggtat acaacaacgg gccgcgacca acatgttaat caaaccactg gtggtcagta
     1441 tcatcaggca acgccccgtc atggaatatg atgcatccca tctcggcaat atggttaacc
     1501 gactggaaga aacattac
//
LOCUS       DAZGSP010000290         1483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZGSP010000290 DAZGSP010000000
VERSION     DAZGSP010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.294"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..768
                     /locus_tag="ACHK74_005325"
     CDS             <1..768
                     /locus_tag="ACHK74_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFP0223955.1"
                     /translation="LISKEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEES
                     GLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGAC
                     TNGLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTA
                     PNRGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIK
                     SFYLGTPSGNFKAIDNHGKEITNRKGLPNVCRVHGNM"
     gene            740..1122
                     /gene="yhhH"
                     /locus_tag="ACHK74_005326"
                     /pseudo
     CDS             740..1122
                     /gene="yhhH"
                     /locus_tag="ACHK74_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631.; frameshifted;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
     gene            1227..1469
                     /locus_tag="ACHK74_005327"
                     /pseudo
     CDS             1227..1469
                     /locus_tag="ACHK74_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
BASE COUNT          521 a          232 c          344 g          386 t
ORIGIN      
        1 cttatcagta aggaaggggc aacagaatgg tgcgcagaat acgatgagtg gggcaacctg
       61 ctgaatgaag agaacccgca tcagctgcag cagcttatcc gcctgccggg gcagcagtat
      121 gatgaggagt ccggcctgta ttacaaccgc caccgctatt atgacccgct gcaggggcga
      181 tatatcactc aggatccgat tggactgaag gggggatgga acctgtatgg atatcaattg
      241 aatccgatat cagacatcga ccccctgggt ttatctatgt gggaggatgc aaaatcgggg
      301 gcatgtacta atggtctttg cggcacacta tccgctatga taggtccaga taaatttgat
      361 tctatagata gcaccgcata tgacgcctta aataaaataa atagccaatc tatttgcgaa
      421 gataaagagt tcgctggttt aatatgtaag gataatagtg gcagatattt ctcaacagca
      481 cctaaccgag gagaaagaaa aggatcatat ccattcaata gcccttgccc taatggtact
      541 gagaaagtat cagcttatca tactcatggt gcagatagtc atggagaata ttgggacgaa
      601 atattttcag gtaaagatga gaaaatagtt aaaagtaaag ataacaatat caagtcattt
      661 tatttaggta cgcccagtgg taattttaaa gcaatagata accacgggaa ggaaataaca
      721 aacagaaaag gattacctaa tgtctgcaga gttcatggta atatgtaaaa aatattgttt
      781 aggaactgtg tcattgtatc tttgtttgtt tttacataca acacatgggc gcagtgtaat
      841 aataatatta aaataatgcg caagtatgaa agtgaaggta aatataccgt tagaaatttg
      901 gttaaaaata aagctataga attggaatta gctgagatat atgttaagaa tcgttatgga
      961 caggatgccg cagaagaaga aaaaccatac gaaatcactg agttaacaac aagttgggtt
     1021 gttgaaggta ccattcactc agaccaaatt gctggtgggg tttttattat agaaataggc
     1081 aaaaatgatg ggagaattct gaattttggc cacggaaaat aagtatgtaa ggataatcaa
     1141 acatcgtgtg cgctgatggc agagtaggtg gaggactcca gacagtcaaa cgatagaaaa
     1201 agatagcctt tatggaggtt cctgcaatgt caaatacata ccagaaaaga aaggcaagta
     1261 aagagtatgg tttatataat aaatgtaaga aactaaatga tgatgaatta tttcgcttac
     1321 ttgatgatcg caattccttg aaaaggattt catctgccag agtattacag ttaagagggg
     1381 ggcaagacgc tgttagattg gcaattgaat tctgcactga caaaaattat atccgtagag
     1441 atatcggagc atttatactc gggcaaatat aaatttgcaa aaa
//
LOCUS       DAZGSP010000291         1418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZGSP010000291 DAZGSP010000000
VERSION     DAZGSP010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.295"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(105..326)
                     /locus_tag="ACHK74_005328"
     CDS             complement(105..326)
                     /locus_tag="ACHK74_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP0223956.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERHKDSFGPYVRLMSVTLN"
     gene            complement(389..865)
                     /locus_tag="ACHK74_005329"
     CDS             complement(389..865)
                     /locus_tag="ACHK74_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP0223957.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(881..1366)
                     /locus_tag="ACHK74_005330"
     CDS             complement(881..1366)
                     /locus_tag="ACHK74_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0223958.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQSQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDGENWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
BASE COUNT          329 a          356 c          413 g          320 t
ORIGIN      
        1 gcactgaccg gtttcggtga agatgtatgc attgatgaga atggcctcat ccacagcggc
       61 gtctgaggtc atctggtcgt actcgtttcc cgggtgacgg aacatcagtt cagggtgacg
      121 ctcatcagcc ggacatacgg gccaaaactg tccttatggc gttcagcaaa cacggccagc
      181 acaccgggaa tatcctgcac ttcacgaccg gtatacgctt cagtgctgcc gtgccagcgg
      241 tatttgccgg tacagaaagg aaaaagacgg gatgccggat gttgttggtg aatacgcatg
      301 gcttcaccac gggtgatgat tttcatagtg tgatacctct gaagacagaa gataaaagtg
      361 aaaacaagcg tgatgtggtt gtgacgggtt aaagcagacc atgttcggca aaggagaaaa
      421 cctggttgcc accgactatc agatggtccg gcacccggat atccaccagg cccagtgcct
      481 gtaccagacg ttccgtgata aggcggtctg ccttgctggg tgtgacttca ccggacgggt
      541 gattgtgcgc cagcaccacg gcagcggcat tgtggtacag ggcgcgttta atcacttccc
      601 ggggatggac ttccgtgcgg ttgatggtgc cggtgaagag ggtttcaccg gcaatcaact
      661 gattctggtt attcagatac agcacccgga attcttcacg ctccagtccc gccatgttca
      721 gaatcagcca ttcccgtgcc gcacgggtgg aggtgaaggc cacgccgggt tcatgaagat
      781 ggcggtccag ggttttcagg gcccgcagaa tgagactgcg ctcaccgggc gtcatctctc
      841 cgggcagaaa ggaaagttgt tgcattgtgc ttctctccat tcagtcgatg atgcgcataa
      901 tggcgctgca ttccggatgc tgcagggcgt aatcccgcaa ccggtaataa tggaccgtca
      961 tggcataaca ctccgtacga caggcatgat gactgtacgt catcagacag gcggcaatgc
     1021 cggcggcttc cgggctcatt tcagcgcggt taccgttcat ggcattgaac agtacccagt
     1081 tttcgccatc atcgtcatcc ggttcggggg ccataaatgc gccaccgttg ttcagtgtgt
     1141 acagattcca gataccaccg cagtagtctt cgcaaagacg gtccatccag ccgaagatac
     1201 ggggctccag ggtcacccac tgtggaatga ggccaaaatg ctgcggccag aagctgatgc
     1261 gctgttcatc agggactatg gtggcaacca gctgagactg gtcattccct gatgcagcgg
     1321 ttacggaaac agaagatgtg gtggaattat gtgaaatggt tttcatgaaa tgactcctta
     1381 aaggacaaat aaatgcgtgg ttttcagggt aatggctg
//
LOCUS       DAZGSP010000292         1348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZGSP010000292 DAZGSP010000000
VERSION     DAZGSP010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.296"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            59..793
                     /gene="nanR"
                     /locus_tag="ACHK74_005331"
     CDS             59..793
                     /gene="nanR"
                     /locus_tag="ACHK74_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFP0223959.1"
                     /translation="MSDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREG
                     EYLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGI
                     VKDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEVY
                     AQYDIEFHSILADIPDNKVLKTIHSAFSEWLIMAKPLEKNKMTYENNKKTHQQHIEIL
                     TAIKEHDCDKAEQLMYNHLYKRIEAS"
     gene            complement(829..>1348)
                     /locus_tag="ACHK74_005332"
     CDS             complement(829..>1348)
                     /locus_tag="ACHK74_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0223960.1"
                     /translation="IRKNADKWHLQRHRIGVLGFSAGGHLASTFVNQICDFKRSSDSK
                     EQEIFAIPDFVGLIYPVISMKDDVTHPDSRKQLLGDNSTSENIIQYSADLNVTSCFPP
                     VFLLHCCDDDLVSIENSLLMYRGLIEKGVSAEMHLYEKGGHGFSVQWATDLPVGGWIK
                     LFMDWMYSHGFK"
BASE COUNT          475 a          279 c          239 g          355 t
ORIGIN      
        1 tattaaaaga aagtgttaaa ttaaaaactc atttgctcta atccatataa atcaatatat
       61 gtccgacaac aaaaaaaccg tgttacctgg gctaacaacg accagaaagc ccattgttac
      121 aaaaaaactt tcagaaatcg ttgccgaaga gcttgaaaga atgatccgcc aggaggagat
      181 aagggaaggt gaatacctgc cttctgaacg tgaacttatg gcattctttg gcgttggaag
      241 accatctgtc agagaggctt tatcatctct cagacataaa gggctaatcc ggtataacaa
      301 gggagaaaaa gccagtgtct gccgtccatc accagaaacc atcattaatg ggttatcagg
      361 cattgttaaa gattttttat ccacagctga tggcatcaag gattttgaaa gactacgcat
      421 cttttttgaa accaaccttg taagatatgc agcaaaaaat gcaacgcctg aggatattca
      481 caggcttgag aaggtgctca gtatccatca taactcactg agaacccccg aagtgtatgc
      541 tcaatatgat attgaatttc atagtatact tgctgatata cctgacaata aagtattaaa
      601 aacaatacac tcagcatttt ctgaatggct gataatggcc aaacctctgg aaaagaataa
      661 aatgacatat gaaaataaca aaaaaaccca tcagcagcat attgaaatac ttacggccat
      721 aaaagagcat gattgtgata aagctgaaca gctaatgtat aatcaccttt ataaacgaat
      781 tgaagcatca tagttactcg acgcttcacc aggtagtcac cataaatatt atttaaatcc
      841 atgagaatac atccagtcca taaacagttt tatccatcct ccaacaggaa gatctgtggc
      901 ccactgaaca ctaaagccat gccctccttt ctcatagaga tgcatttcag cagaaacgcc
      961 tttctctatt aggccacggt acatgagcag actgttttca attgacacaa gatcatcatc
     1021 acaacaatgg agtaaaaaca caggaggaaa acatgatgtg acattaagat ccgcactata
     1081 ttgtattata ttttctgagg tactattgtc ccccagtaat tgcttcctgg agtctggatg
     1141 tgtcacatca tctttcattg atattacagg ataaattaac ccgacaaaat cgggaatagc
     1201 gaaaatctct tgttctttac tgtcagaaga acgtttgaaa tcacaaatct gattcacaaa
     1261 cgttgaggcg agatgccccc cagcagaaaa tcctaataca ccaatacgat gacgctgtag
     1321 atgccattta tcagcattct tacgaata
//
LOCUS       DAZGSP010000293         1336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZGSP010000293 DAZGSP010000000
VERSION     DAZGSP010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.297"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..971
                     /locus_tag="ACHK74_005333"
     CDS             <1..971
                     /locus_tag="ACHK74_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP0223961.1"
                     /translation="NEKIDFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRVKQLARR
                     KTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHIAKKLLP
                     YIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLVARVKVA
                     LEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCVGNSSER
                     VPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEPEEDADD
                     LSVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
BASE COUNT          386 a          257 c          332 g          361 t
ORIGIN      
        1 ttaatgaaaa aatagatttc tggtatgtga tagccctcgc aggacagtct aatggtatgg
       61 cctacggtga aggtattccc ctgccagata cactcgacaa acccgagtcc cgtgtaaaac
      121 aactggctcg tcgaaaaaca atcacaccag gaggaaaaga gtgtaaattc aatgagatta
      181 ttccggcaga tcactgcctg catgatgttc aggatatgag tggatatcac catccggctg
      241 ctgatttgca taaaggggaa tatggttgtg tcggacaggg attgcatata gctaaaaagt
      301 tactgccata catacctgag caagcaggga tattactggt tccttgttgc agaggaggtg
      361 ccgcctttac tgttggagct gaaggtatgt atgtacctga tacaggggca acagctgatg
      421 caatgcggtg gggcaccgga acagcactct atgaggactt agtagcccgg gttaaggtag
      481 ctctggaata taacagaaag aataaactgc tatctgtctg ctggatgcag ggggagtttg
      541 atcttatgtc accagattat gaaaagcatc ctgacttgtt ttatcagatg gtaacttctt
      601 tcaggtctga gttatctgaa tactcatctc agtgtgttgg taattcgtca gaaagagttc
      661 catggttgtg tggtgataca acctggtact ggaaggaatc ttatcaaaaa gaatatgatt
      721 ttatatatgg acattacaga caacggactg atgatgaaat acattttctc agctttcagg
      781 atagtaaccg acatgaatta acgaatgagc cagaagaaga tgccgatgat ttatctgtcg
      841 ggtatctggg gtcatcatgg aggacagaat taagctggac aacgtcacag agaagtacac
      901 actttaattc aatggccaga aggggagtta ttgctgaatg ttatgcgcaa aaaataagaa
      961 attatttgta gacaagttca ggatatatct acagctctga catgattctc tgagagacat
     1021 gacggagtgg tatggagttg tcggtatcaa caccaagata gctggtcgtt accgtttgat
     1081 atcactggtt ctgatactat gtggacggta gtgtgtcacg aaggcacgca ccagggacgt
     1141 gagtccctgt aatatcatcg acagtcccat tatggcatgt ctgcacctac aacgtcattt
     1201 cgaggtcggc tcagtgttca ctcgagtctt atatcactga cgccgacact ccatccagag
     1261 tatctctgga cggaaacatc acctattagt tcttttttct gtcagggcca aaacattcag
     1321 tcgatgtggc gcaggt
//
LOCUS       DAZGSP010000294         1317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZGSP010000294 DAZGSP010000000
VERSION     DAZGSP010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.298"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            9..506
                     /gene="tssB"
                     /locus_tag="ACHK74_005334"
     CDS             9..506
                     /gene="tssB"
                     /locus_tag="ACHK74_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFP0223962.1"
                     /translation="MSSSFQNEIPKARVNLKLDLHTGGGQKKVELPLKLLVAGDFSNG
                     QEERPLSERIKMDVNKNNFNAVLADISPSVRLNVENTLAGDGTEESISLTFRNMNDFT
                     PEQVARQIPRLKAMLAMRSLLRDLKANLLDNATFRKELEKILRDPELSQALRDELSAL
                     VPEQV"
     gene            527..>1317
                     /locus_tag="ACHK74_005335"
     CDS             527..>1317
                     /locus_tag="ACHK74_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP0223963.1"
                     /translation="MLMSVQKENNVAGNAVSETHSGDSVYASLFEKINLNPVSALSAL
                     DIWQDPQAMSEASADERLTAGMQVFMECLAKAGAPVEKLDKALIDHHIAELDYQISRQ
                     LDAVLHHPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQDFEDAPEIIQS
                     GLYLQTYVAEYDTPGGEPIAALISAWEFDASAQDVALLKNISRVAASAHMPFIGSVGP
                     AFFRKDTMEEVAAIKDIGNHFERAEYIKWNAFRETDDARYIGLVMP"
BASE COUNT          332 a          284 c          383 g          318 t
ORIGIN      
        1 agaacgtcat gagcagttcg tttcagaatg agatcccgaa ggcaagggtg aatctgaaat
       61 tagacctaca tacaggtggt gggcagaaga aagtggagct tccgctcaaa ctgctggttg
      121 ccggtgattt cagtaacgga caggaagagc gcccgctgtc agagcgcata aaaatggatg
      181 ttaataaaaa taactttaat gctgttctgg ctgatatctc gccgtcagta cgcctgaatg
      241 tggagaacac acttgccggt gatggtactg aagagagcat cagcctgact ttccgtaata
      301 tgaacgattt cacgccggag caggttgccc gacaaattcc ccgtcttaaa gccatgctgg
      361 ccatgcgcag ccttctgcgg gatctgaagg cgaatctgct ggataacgcc actttccgta
      421 aagaactgga aaaaattctg cgggatcccg aactgagtca ggccttacgt gacgaactga
      481 gtgcactggt gccggaacag gtgtgattaa cggattaaga gagagcatgc tgatgtctgt
      541 acagaaagaa aataatgtgg cgggaaatgc ggtttctgaa acgcattccg gcgacagcgt
      601 atatgcttcc ctgtttgaaa aaattaatct gaatccggta tcagccctga gtgcgctgga
      661 tatctggcag gatccgcagg ccatgtcaga ggcatcagca gatgaacgtc tgaccgcagg
      721 gatgcaggtg tttatggaat gcctggcgaa agcgggagca ccggtggaga aactcgataa
      781 ggcgctgatt gaccatcata ttgcagagct tgattatcag atcagccgtc agctggatgc
      841 cgtgcttcat catccggaat ttcagaaagt ggagtccctg tggcggggcg tgaagtctct
      901 ggtggataaa acggacttcc gccgtaatgt gaaaattgaa ctgctggatc tgtccaaaga
      961 cgatctgcgt caggattttg aggatgcgcc ggaaattatt cagagtggtc tttatctgca
     1021 gacttatgtg gcggagtacg atacgccggg tggtgaaccc attgcagccc tgatttctgc
     1081 ctgggagttt gatgcgtctg cgcaggatgt ggcgctgctg aaaaatattt cccgggtggc
     1141 tgcgtccgcg catatgccat ttattggttc agttggcccg gctttcttcc ggaaggatac
     1201 gatggaagag gtggccgcca ttaaggatat tggcaatcac tttgagcgtg ccgagtacat
     1261 caagtggaat gctttccgtg aaacggatga tgcccgctat attggtctgg tgatgcc
//
LOCUS       DAZGSP010000295         1269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZGSP010000295 DAZGSP010000000
VERSION     DAZGSP010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.299"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..899)
                     /gene="tssI"
                     /locus_tag="ACHK74_005336"
     CDS             complement(<1..899)
                     /gene="tssI"
                     /locus_tag="ACHK74_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFP0223964.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGR"
BASE COUNT          325 a          324 c          323 g          297 t
ORIGIN      
        1 cgtcccggcc atatctccgg cgagcgactc attccccgcg cggtttctgc attgtttcgc
       61 cagccgtcca tctgccagcg ggcaaagttc tgcccgtggg cacccttgaa acgtccgggg
      121 tagtcataca cttcatactg cgtgcgctgg taatcctggt actggccttc ctgatcaaaa
      181 cgccctgccc agccgggtcg tttaaaggtg tagtccttgg tcaccacgga agaagggcgg
      241 atttgtgcgc tgtagcggaa ctggctgatg cagagggtgc tcacctcggt acgggtgttc
      301 ggattccagg ggatctcaaa ggactccggc agataacgca cggtgtcgca caggaccagg
      361 ctctggtcgg tacttttttg cgcgtgctcc tcataaaaga agatgccttc ctccgccgcc
      421 atccggcaca ggaaatcgta atcggtttcg ccgtactgga cacaaaactc acgggaagga
      481 tgcggctcgc tgaacagcgg actccactcg gtcacaccgt tttccttcag gatcgtagcg
      541 aggatgcttt cgatgtcctc attctggaag atacggaagt tctggcgcag ccctgtgcgc
      601 cacagcggcg ggcacacctt catgctgtac agcatctggt ttttgtcgtt ctcccccagt
      661 tcaaaccagg tcaccacacc tttcacccgg cgctgtacgt catcgccctg ccataccgtc
      721 aggtaggcca ttttgtccag cacctgcgca aattcaaggg aaagaaactg ctgactgacc
      781 agagaaaggt cgagggaaaa aagtgaagag agtgactggg tgagatgaaa ggaaaccacc
      841 gcaaacgcgt ccggtggcag gccgtccact tcaagtgtga aacgtaatcc ggttgacata
      901 gcaacctccg tgaaaatgaa taataatccg cacatattgc atgtacaaca tgtcgggcgg
      961 atgagataag cgagtcgaca tctggcgtta ccggggaata tcagaaaaat gtcatggtta
     1021 tgtgatgatt tagtctggga ttttacttca gagaaacggg tgtctgttta gtcaacaaaa
     1081 taatcacaaa cgcaacggat gggttctcga cgttattcac gacaggctcg tacattacag
     1141 cgagtaataa aaaacctgat atgcaaagtc aaaccaatat tcataatgtc tttttattca
     1201 caacaataaa taattcaata acaatcattg aaatgaatga tgatttgtat taggaatata
     1261 cgctaccgc
//
LOCUS       DAZGSP010000296         1268 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZGSP010000296 DAZGSP010000000
VERSION     DAZGSP010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.300"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            34..1247
                     /locus_tag="ACHK74_005337"
     CDS             join(34..325,325..1247)
                     /locus_tag="ACHK74_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP0223965.1"
                     /translation="MTKNTRFSPEVRQRAVRMVIESQNDYDSQWAAISSIAPKIGCTP
                     ETLRTWLRQYERDAGAGDGGLSTTERQRLKELERENRELRRSNDILRQASAYFGEGGV
                     RPPVEKIAPLVDTLRGAHGVGPVCHELAVAPSTYYRHRAHQRHPDKRSQRAQRDDLLK
                     PEIQRVYDENHSVYGVRKVWRQLLREGVTVARCTVERLMKVMGLAGVRRGKKVRTTVS
                     RKEAAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFAYVAFIIDVFAGVIVGWRVSSS
                     MKTTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTQRLKDAELLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHIPPAEAEKA
                     YYASIGNGGLAA"
     misc_feature    315..431
                     /locus_tag="ACHK74_005337"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          287 a          334 c          409 g          238 t
ORIGIN      
        1 gagactaaac tccttgagag agaggtaaac aggatgacta aaaatacgcg cttttcccct
       61 gaggtccgtc agcgggccgt tcgtatggtt atcgaaagcc agaatgacta tgactcgcag
      121 tgggcggcca tcagctccat cgcccctaaa atcggctgta cgcctgagac cctgcgtacc
      181 tggctgcgtc agtatgagcg cgatgccggt gcaggtgacg gcggactgag caccactgaa
      241 cgtcagcgtc tgaaggaact ggagcgtgaa aaccgcgaac tgcgccgcag caacgatatc
      301 ctgcgccagg cttccgcgta ttttgcgaag gcggagttcg accgcctgtg gaaaaaatag
      361 cgcctctggt ggataccctg cgtggtgcgc acggggtcgg accggtatgc cacgagctgg
      421 ctgtcgcccc gtcaacgtat taccggcatc gtgcgcatca acggcatccg gataaacgca
      481 gtcagcgggc acagcgtgat gacctgctga agccggagat acagcgcgtg tacgatgaaa
      541 accacagcgt gtacggtgtg cgcaaggtct ggcgccagtt gttgcgtgag ggggtgaccg
      601 tggccaggtg cacagtagaa cggctgatga aggtgatggg gcttgccggt gtgcgccggg
      661 gtaaaaaggt ccgcaccaca gtcagccgga aagaagccgc tgcgggtgac cgggtgaacc
      721 gccagttcgt ggcagaacgc cccgaccagc tgtgggtggc tgacttcacg tatgtgagca
      781 cctggcaggg cttcgcttac gtggcgttca tcatcgatgt gttcgccggc gtcattgtgg
      841 gctggcgggt ctcatcgtcg atgaaaacga cgttcgtgct ggatgcactg gagcaggcgc
      901 tgtgggcccg tcgcccgtcg ggcaccatcc atcactcaga taaggggtca caatatgtgt
      961 cgctggcata cactcagcgg cttaaggatg cagagttgct ggcgtcaacc ggcagcacag
     1021 gcgactcata tgacaacgcg atggcggaga gcatcaacgg actttataaa gcggaggtga
     1081 tacatcgtaa gagctggaaa aaccgcgccg aagtggaact ggccacactg acgtgggtgg
     1141 actggtacaa taaccgacgg ttgctggaac gactgggtca tatcccgccg gcagaagcgg
     1201 aaaaggcata ttacgcttcc atcggaaatg gtggtctggc agcctgagtt cacagataaa
     1261 acactctc
//
LOCUS       DAZGSP010000297         1266 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZGSP010000297 DAZGSP010000000
VERSION     DAZGSP010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.301"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..380)
                     /locus_tag="ACHK74_005338"
     CDS             complement(<1..380)
                     /locus_tag="ACHK74_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223966.1"
                     /translation="MSPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIEMVTLLNALQPGGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQEISTGNINVN"
     gene            complement(402..941)
                     /gene="yhaB"
                     /locus_tag="ACHK74_005339"
     CDS             complement(402..941)
                     /gene="yhaB"
                     /locus_tag="ACHK74_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFP0223967.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPTHFNKTCIT
                     SDYWQILFFSPDHFNNDFY"
     gene            complement(1197..>1266)
                     /locus_tag="ACHK74_005340"
     CDS             complement(1197..>1266)
                     /locus_tag="ACHK74_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFP0223968.1"
                     /translation="QLINVNFFSSDRTSFCECNSFP"
BASE COUNT          381 a          220 c          242 g          423 t
ORIGIN      
        1 caattaacat tgatattgcc tgtagatatt tcctggtttt ttaacggttc attaatggta
       61 agttcgcagg tattgtccga tcgttgaata atttgggcgt tataacctaa aaaatccaga
      121 cgaagagacg agtcattata aagaattaca ttttgtaagt acgctaattc tttttgaaaa
      181 tcaccagtga taatgcattt tccacctggt tgtaacgcgt taagtaaagt gaccatttca
      241 atagaggtct gttttagttt ttctatacct ggtgctagtg cttcgaggtt atttcctatg
      301 gggctttcag gaaggtcatt catttttcga cggactaaat cactactaat atcattacca
      361 attgatgaaa caggggacat tattttatcc tttataaata atcaataaaa atcattatta
      421 aaatgatcgg gtgaaaaaaa gagaatttgc caataatctg aagtaatgca ggttttatta
      481 aagtgcgtag ggggtgttaa gatggcttct ttttgggtga actttgcgca atacttagaa
      541 atcgtctcct taatacattc cagctctttt ttattgacgg ctgagcaagt acattgttca
      601 ccaaaaggtg caaaagtgcc tacatctaat gtttgtatga tataatcgtc atgttctcct
      661 gtagaaatat ctataatgaa tggactcata ttttccggtt ggattcggat catgacagaa
      721 ggaacatcgc cttgatagaa attcaatgca gtaattgcag ataatactct ccgcagttca
      781 ttacttatac tgtaaatttt attaacgtca ttggtggaga caatttctgc aaactttctt
      841 tttaccgtcc aataatcaat atttctattg tgattgttaa ccactgcgtg cattggcggg
      901 attgaagggt gaaaaatatg tgcaattggg aagcctttca ttgatgcctg ccttgtcatc
      961 atgacaaaca ataatagatg cttattttcc tcgcagggtt ctttcgcact atcaaaaaac
     1021 gctcatttac ccggcgttac agtaaacgaa gcaacattct gtggcctacg acgatcaaaa
     1081 gctctgtgaa aatttccctt gttgtctttg tctgacctta tcgcggcgat cagcaattat
     1141 tcgttttgtg atcctcaaca caaaatgcta ttcgtcgcct atgaggggag aagaatttat
     1201 ggaaaactat tgcattcaca aaatgacgtc ctgtcagaag agaaaaagtt gacgttaatc
     1261 aattgt
//
LOCUS       DAZGSP010000298         1265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZGSP010000298 DAZGSP010000000
VERSION     DAZGSP010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.302"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            63..>1265
                     /locus_tag="ACHK74_005341"
     CDS             join(63..384,384..>1265)
                     /locus_tag="ACHK74_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP0223969.1"
                     /translation="MTGILLGQELRKRKTPQEKIAIIQQTMEPGMTVSHVARLHGIQP
                     SLLFKWKKQYLEGSLTAVAAGEDVVPASELAAAIKQIKELQRLLGKKTMEVEILKEAV
                     EYGQSPKMDSACALVANGQGITLVSRILGVSRAQLAQRMKTSGNKPNKRRRRCDEAAD
                     AEVLARILDIISDMPTYGYRRVWAILRRQSRNKGVPFVNAKRVYRIMSENNLLLHDKP
                     SRPQREHKGRISVKESDQRWCSDGFEFGCDDGAKLRVTFALDCCDREAIDWAASTGGY
                     DKATVQDVMLGAIEKRFGDKGAERPIQWLTDNGSAYTAHETRQFARELNLEPCTTAVS
                     SPQSNGMAERFVKTMKEDYIAFMPKPNVRTALHNLAVAIEHYNETHPHSALGYLSPRE
                     YRRQRVTST"
BASE COUNT          362 a          269 c          353 g          281 t
ORIGIN      
        1 ggatgtatct taaaataaga gataggcttg gatatatgtc taacactagt gctaattttg
       61 agatgactgg aattttgctg gggcaggagc ttcgtaaacg taaaactcct caggaaaaaa
      121 tcgccattat tcagcagaca atggaacccg gtatgacggt gtcccatgtg gcgcgtttgc
      181 atggtattca gcccagcctg ttgtttaagt ggaaaaaaca atatctggaa ggaagtctca
      241 ctgctgttgc tgccggagag gatgtcgttc ctgcctcaga actggctgcc gctataaaac
      301 aaataaaaga gttacagcgc ttgctgggta agaaaaccat ggaagttgag atcctcaaag
      361 aagcggtgga gtatggtcag tcgcgaaaat ggatagcgca tgcgcccttg ttgccaacgg
      421 acaaggaata accctggtaa gccgtattct gggcgtgtcg cgtgcgcaac tggcacaacg
      481 gatgaaaaca tctggtaata agccgaataa acgtcgtcgc agatgtgatg aggctgcgga
      541 tgcggaagtg ttagcacgta tccttgatat catcagcgat atgccgacat atggctatcg
      601 tcgtgtctgg gccattctca gacgacagtc ccgtaacaaa ggagtgccgt tcgtgaatgc
      661 aaagcgcgtt tatcggataa tgagcgaaaa taacctgttg ttacatgaca aaccttcacg
      721 gccgcagcgg gaacataaag gccgaatatc ggtgaaggaa agcgaccagc gctggtgttc
      781 cgatggcttt gagttcggtt gtgatgatgg cgcaaaactg cgtgtcacgt tcgcactgga
      841 ctgttgcgat cgtgaagcca tagactgggc cgcaagcacg ggaggttatg acaaagcaac
      901 ggtgcaggat gtcatgttag gagcaataga aaaacgcttt ggcgataaag gagcggaacg
      961 gccaatccag tggctgactg ataacggttc ggcttataca gctcatgaaa cacggcagtt
     1021 cgccagagag ttgaatctgg agccctgcac gacggcagtc agtagccctc agagcaatgg
     1081 aatggcagaa aggttcgtga aaacgatgaa agaagactac atcgcgttca tgccgaaacc
     1141 gaatgtaaga acggcattgc ataaccttgc agtggcgatc gagcattaca atgaaaccca
     1201 tccgcatagt gcgttaggtt atctctcacc aagggaatat cgacgtcagc gggtaacgtc
     1261 aactt
//
LOCUS       DAZGSP010000299         1251 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZGSP010000299 DAZGSP010000000
VERSION     DAZGSP010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.303"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            183..1163
                     /locus_tag="ACHK74_005342"
     CDS             183..1163
                     /locus_tag="ACHK74_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HFP0223970.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVINPALGKAFAQSEGLRNKTD
                     TVDARMLAEFCRQKRPAAWEAPHPLERALRALVVRHQALTDMHTQELNRTETAREVQR
                     PSIDAHLLWLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGL
                     KDRFAHARQFAAFAGLTPRRYESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEW
                     GRAFRDRLAANGKKGKVILGAMMRKLAQVAYGVLKSGVPFDASRHNPVAA"
BASE COUNT          306 a          309 c          371 g          265 t
ORIGIN      
        1 cctccagtgc agagaaaatc ggccagtttt ctctgcctgc agtccgcatg ccgtatcggg
       61 ccttgggttc taacctgttg cgtagattta tgcagcggac tgcctttctc ccaaagtgat
      121 aaaccggaca gtatcatgga ccggttttcc cggtaatccg tatttgcaag gttggtttca
      181 ctatggaaca tgaacttcat tatatcggta tcgacaccgc taaagagaaa ctggatgtcg
      241 atgtgttgcg tcctgatggt cgtcatcgca ccaaaaaatt cgctaacacc actaaagggc
      301 acgatgagct ggtgagctgg ctgaaaggtc acaagattga ccatgcgcat atctgcatcg
      361 aagcgaccgg cacctatatg gaacctgtcg ctgagtgcct ttacgatgct ggctacatag
      421 tgtcagtcat taatcctgcg ctgggtaaag ctttcgctca gagtgaagga ctgcgtaaca
      481 agactgatac cgtggatgcg cgcatgctgg cagagttctg tcgtcagaag cgccctgcag
      541 cctgggaagc gcctcacccg cttgaacgcg cgttgcgtgc cctggtagtc cgccaccagg
      601 cgctgacaga tatgcacacg caggaactga atcgcactga aacggcgcgg gaagtccaga
      661 gaccgagcat tgatgctcac cttctgtggc ttgaagcaga gctgaagcgt cttgagaagc
      721 agataaaaga cctgacagac gatgatccgg atatgaaaca ccgcaggaaa ctgctggaaa
      781 gcatcccggg tatcggagag aaaacatctg cggtattgct ggcttatatc ggtctgaagg
      841 accgcttcgc ccatgccaga cagttcgccg cttttgcggg tctgacacca cggcgttatg
      901 aatcaggtag cagtgtgaga ggggcgagcc ggatgagtaa ggccggacat gtgtcgcttc
      961 gcagggcgtt gtatatgccc gcaatggtag ccaccagtaa gactgagtgg ggacgggcgt
     1021 tccgcgaccg tctggcggct aatggcaaga aaggaaaggt gattctcggc gcgatgatgc
     1081 gcaagctggc acaggtggcg tatggagtgc tgaagtcagg cgtgccgttc gatgcgtcac
     1141 ggcataatcc ggtagcggcg taaaaatcgc ggaagggatg aaaaaaacag cgcctgacgg
     1201 cgctgtgtct ggcatgcctg caatccggga aaccggacca ggaaaaaact t
//
LOCUS       DAZGSP010000300         1248 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZGSP010000300 DAZGSP010000000
VERSION     DAZGSP010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.304"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..899)
                     /gene="tssI"
                     /locus_tag="ACHK74_005343"
     CDS             complement(<1..899)
                     /gene="tssI"
                     /locus_tag="ACHK74_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFP0223971.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGR"
BASE COUNT          318 a          314 c          317 g          299 t
ORIGIN      
        1 cgtcccggcc atatctccgg cgagcggctc attccccgcg cggtttctgc gttgtttcgc
       61 cagccgtcca tctgccagcg ggcaaagttc tgcccgtggg cgctcttgaa acgtccgggg
      121 tagtcataca cttcatactg cgtgcgctgg tagtcctggt gctggccttc ctgttcaaaa
      181 cgtccggccc agccggggcg tttaaaggtg tagtctttgg tcaccacgga agaagggcgg
      241 atttgtgcgc tgtagcggaa ctggctgatg cagagggtgc tcacctcggt acgggtgttc
      301 gggttccatg ggatttcaaa agattcgggc agatggcgga ctgtgtcgca cagcaccagg
      361 ctctggtcgg tacttttgta agcatgctcc tcataaaaga agatgccttc ctccgccgcc
      421 atccggcaca gaaaatcgta atcagtctca ccgtactgga cacaaaactc acgggaagga
      481 tgcggctcgc tgaacagcgg actccattcg gtcaccccgt tttcctgcaa catcgtgccg
      541 aggatgcttt tgatgtcctc gttctggaag atacggaagt tctggcgcag accggcacgc
      601 cacagcggcg ggtgcacctt catgctgtac agcatctggt ttttgtcgtt ctccccgagt
      661 tcaaaccagg tcaccacgcc tttcacccgg cgctgtactt catcgccctg ccatatcgtc
      721 aggtaggcca ttttgtccag cacctgcgca aattcaaggg agagaaactg ctggctgacc
      781 agggagagat cgagggaaaa gagcgaagag agtgactggt tcagatgaaa ggaaaccacc
      841 gcaaaagcat ccggcggcag gccgtccact tccagtgtga aacgtaatcc ggttgacata
      901 gcaacctccg gtaaaatgaa taatgatccg taccgtacaa ctgcggataa tgtgataagc
      961 gggacgactt cagacgttat tagggaagga acagaaaatg ctatggttat gtgagaattt
     1021 tgtttaaata ttactttaca gaaacgggtg tctgttcagt caaaaaaata atcacaagct
     1081 caacctcttg tttcttggcg ttattcagga caggtacgca tatcacaacg agtaacaaaa
     1141 acgcaaagtt aaaccaatat tcataatata tttttattca cagcaataaa taatccaatt
     1201 aacattatta aaatgaatga ttgcataaga aatatatgct accgctcc
//
LOCUS       DAZGSP010000301         1224 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZGSP010000301 DAZGSP010000000
VERSION     DAZGSP010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.305"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            49..324
                     /gene="yddL"
                     /locus_tag="ACHK74_005344"
                     /pseudo
     CDS             49..324
                     /gene="yddL"
                     /locus_tag="ACHK74_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            363..>1224
                     /gene="yddK"
                     /locus_tag="ACHK74_005345"
     CDS             363..>1224
                     /gene="yddK"
                     /locus_tag="ACHK74_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFP0223972.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNDLFPNLNTLLINNNLLSEIKIFYSNFCNVQTLNAANNQLKYINL
                     DFLTYLPSIKS"
BASE COUNT          437 a          233 c          190 g          364 t
ORIGIN      
        1 aattcgttta gaaaatacgt ttaacgataa ttaaaaggat tctgttaaat gaaattaaaa
       61 atagttgcgg tggttgtaac tggtttgtta gctgcgaacg tagcacacgc tgccgaagtc
      121 tataacaagg atggtaataa actcgacctt tatggcaagg ttaccgctct acgttatttt
      181 actgatgata agcgtgacga tggtgataaa acttatgccc gtctcggctt taaaggagaa
      241 acgcaaatca atgatcaaat gattggtttt ggtcactggg aatatgattt taaaggctat
      301 aacgatgaag ccaacggctc gcgcggcaag aatctcttat tgagttaagt gcccatcaca
      361 atatgatcac tgaccttata ttacacaatc atcccaggat gaaaacaatc actttaaacg
      421 acaaccatat tgcacattta aacgccaaaa acactacaaa actggaatat ttaaacttaa
      481 gcaataacaa tttactgcca accaatgaca ttgatcaact aatatcatca aaacatcttt
      541 ggcatgtatt agttaacggc atcaacaatg atccacttgc acaaatgcag tactggactg
      601 cagtaagaaa tataattgat gacactaatg aagtgaccat tgatttatca ggacttaatt
      661 taaccactca accaccaggg ctgcaaaact tcacctctat caatcttgat aataaccaac
      721 tcacacattt tgatgcaacc aactacgata gactcgtaaa gctaagtctg aatagtaatg
      781 ctcttgagtc aataaatttt cctcaaggca gaaatgtaag tattacacat atatctatga
      841 ataataatgc tctcagaaat attgatatag ataggctttc atcagttact tattttagtg
      901 cggcacataa tcaactagag tttgtgcaat tagaatcttg cgaatggctg caatacctga
      961 atctcagcca taatcaatta actgatattg ttgcaggtaa taaaaatgaa ctcttactgc
     1021 tggatctatc ccataataaa ctaacaagtt tacacaatga cttatttcca aacttgaata
     1081 cgttacttat taacaacaat ttgctttctg aaattaaaat attctatagc aacttctgca
     1141 atgttcagac attaaacgct gctaacaacc agttgaaata tataaatctt gatttcctga
     1201 cttatcttcc atctatcaaa agtt
//
LOCUS       DAZGSP010000302         1203 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZGSP010000302 DAZGSP010000000
VERSION     DAZGSP010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.306"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            175..1194
                     /gene="galE"
                     /locus_tag="ACHK74_005346"
     CDS             175..1194
                     /gene="galE"
                     /locus_tag="ACHK74_005346"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP0223973.1"
                     /translation="MAILVTGGAGYIASHTILSLIEQGNEVIIIDNLSNSYYDSLLKI
                     KEITGCDCLFYKGDILDKKLLLKIFDENNITAVMHFAGAKSVSESVIRPLQYYRNNVS
                     GTFSLVEAMEESGVENLIFSSSATVYGEPKIIPVNESCAIGGTTNPYGTSKLFVENFL
                     RDYSKASPNFKTIVLRYFNPIGAHSSGKIGEDPNGIPNNLMPFICQVAIGKQKTLKIY
                     GNDYPTKDGTGIRDYIHVMDLAEGHVAALNNINQGANYRVYNLGTGIGYSVLELLEAF
                     QKVTTRRVPYVFTKRRSGDIAECWSDPTKAYEELGWKARRGLEDMIRDAWNWQQKNPN
                     GFKKV"
BASE COUNT          386 a          163 c          247 g          407 t
ORIGIN      
        1 gaaagtggtt gcgtattata aattgttcat attgacgttt aagtctttta taactccatg
       61 cttgcatgtt aaaaatattt cgctatatat tatggtaggt ggtttttatc taggcagatt
      121 tgtctattta actctaaatc taaatcgctt aagaaagtat gagtaggagt atttatggct
      181 attctcgtta caggtggtgc tggttatatt gcatcacata ctatactgag tttgatagag
      241 cagggtaatg aagtaattat tattgataat ttatcaaatt catattatga ttcgttattg
      301 aaaataaaag agattactgg atgtgattgt cttttttata aaggagatat ccttgacaaa
      361 aaactcttgc taaaaatatt tgatgaaaat aatatcactg ctgtgatgca ttttgctggt
      421 gcaaagtctg taagtgaatc tgttatcaga cctttgcaat actatcgaaa taatgtctct
      481 ggcacttttt ctttagttga ggcaatggag gagtctggtg tagaaaattt aatatttagc
      541 tcttcagcaa ctgtctatgg tgagccaaag attattcctg tgaatgaaag ttgtgctata
      601 ggtggtacta caaatccgta tggaacgtcc aagttatttg tagagaactt tttgcgggat
      661 tattctaagg catcccctaa ttttaaaact atagtattaa gatattttaa ccctatcggt
      721 gctcactcat ctggaaaaat aggtgaagat cctaacggaa ttccgaataa cttgatgccc
      781 tttatatgcc aggttgctat cggtaaacaa aaaacgctca agatctacgg aaatgattat
      841 cctactaaag atggtactgg gattcgcgat tatattcatg ttatggatct tgctgaaggt
      901 catgttgctg cattaaataa tattaatcaa ggagctaatt atagagttta caaccttgga
      961 acaggaattg gatactctgt gttggaatta ttagaagcat ttcagaaagt tacaactcgg
     1021 agagttccct atgtttttac taaaaggcgt tcaggtgata ttgctgaatg ttggtctgat
     1081 cctaccaaag cgtatgaaga attaggatgg aaagcaaggc gagggttaga agatatgatt
     1141 cgtgatgcat ggaattggca gcaaaaaaat ccaaatggat ttaagaaagt ttaataaaaa
     1201 tga
//
LOCUS       DAZGSP010000303         1168 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZGSP010000303 DAZGSP010000000
VERSION     DAZGSP010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.307"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1168
                     /locus_tag="ACHK74_005347"
     CDS             <1..>1168
                     /locus_tag="ACHK74_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S6"
                     /protein_id="HFP0223974.1"
                     /translation="GGGLYIQGHPVVHAVSSEAVATALRKQGDNSVLTQPVSFAQKDW
                     ESRTFSIGQLKLKGAAFSLSRNATLTGDIDADNATMVLGSDSLYLDMKDGTGSSSAPV
                     KGTSAVGGASGSSTFRGNVNMRHSALTVCDHFTGSITASDSRIAVSSENVRLEGNSRL
                     TSSALTVSDGGRLHVKGGLETDGGVTLDRGTLLVDGGSVRNDVYERLLAWSEERGGLN
                     GSGEYDFMTGAAGLLRGYVRGSAGNVNLQNAAWMMTGNSSVKHLESSGSALYFSRPGG
                     EFHTLTAGSMDISDSVLVMRTDLNNSDQLRVTESLRGKNNLLLVDFTERSDGQKALNI
                     PLVTAPAGTSADVFSVKTRDTGFSHITPVVRAEQGTGGTAWQLNVVQPETAAEPV"
BASE COUNT          252 a          250 c          403 g          263 t
ORIGIN      
        1 ggcgggggac tgtatatcca ggggcatccg gttgttcatg ctgtcagcag tgaggcggtt
       61 gcgactgcat tgcgaaagca gggtgataat tctgttctga cgcagccggt atcatttgct
      121 cagaaggact gggagagcag aacgttcagc atcggtcaac tgaaactgaa gggggcggca
      181 ttcagccttt cccgcaatgc gacactgacg ggagatattg atgctgataa tgccaccatg
      241 gtgctgggaa gtgactcgct atatctggat atgaaggacg gcacggggag cagcagtgcg
      301 ccggttaagg ggacgtctgc cgtgggcggt gcatccggta gcagtacatt tcgcgggaat
      361 gtgaatatgc gtcattctgc cctgacggtc tgcgaccatt ttactggcag tataacggca
      421 tcggacagcc ggatagctgt gagttcagag aatgtgcgtc tggaagggaa ttcccgcctg
      481 acctcttctg cacttactgt cagtgacggc gggcgtttac atgtgaaggg gggactggag
      541 acagatggtg gtgtgacact ggacagagga acccttttgg tggacggggg aagtgtcagg
      601 aatgatgtat atgaacgtct gctggcctgg tctgaggagc gtggcgggct gaacggaagc
      661 ggtgagtatg attttatgac gggggcagct ggtctcctgc ggggttatgt cagagggagt
      721 gcaggtaatg tgaatctgca gaatgcggcc tggatgatga cgggaaactc atccgtgaag
      781 catcttgagt catcgggaag tgctctgtat ttcagcaggc cgggcgggga gttccatacc
      841 ctgacggcag gcagtatgga tatcagcgac agtgttctgg tgatgcggac ggaccttaac
      901 aactcagacc agcttcgggt gacagaatcg ctgcggggta agaataacct gttgttggtt
      961 gattttactg agcgttctga cgggcagaag gcgctgaata tccctctggt gacggcgccg
     1021 gccggtacca gtgcggatgt gttcagtgtg aaaacgcgtg ataccggctt cagtcatatc
     1081 acacctgttg tcagggctga acaggggacg ggcggcacgg cctggcagct gaatgttgtg
     1141 cagccggaaa ctgccgcaga gcctgtgg
//
LOCUS       DAZGSP010000304         1167 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZGSP010000304 DAZGSP010000000
VERSION     DAZGSP010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.308"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..502
                     /locus_tag="ACHK74_005348"
     CDS             <1..502
                     /locus_tag="ACHK74_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0223975.1"
                     /translation="NVNSDNILDPEFEQQCVQMLEQLGKEKIKLILELSERKKLQDTN
                     EVHTIVDKLQKHNVLFALDDFGVEYSTFHYLQDYSVDFIKLDKSFIQQCESNKISEAI
                     VDSIAMLAKRLHIKVVAEGIETIEQAKAMKNKGIEYLQGYLFSPPVSGDVFIHKWILS
                     TEKRSN"
     gene            complement(512..1098)
                     /locus_tag="ACHK74_005349"
                     /pseudo
     CDS             complement(512..1098)
                     /locus_tag="ACHK74_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1078..>1167
                     /locus_tag="ACHK74_005350"
     CDS             1078..>1167
                     /locus_tag="ACHK74_005350"
                     /inference="COORDINATES: protein motif:HMM:NF024169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /protein_id="HFP0223976.1"
                     /translation="MTGYWALSVHENTHKPGVKEQITEMAFNGA"
BASE COUNT          355 a          222 c          242 g          348 t
ORIGIN      
        1 caatgttaat tcagacaata ttctggaccc tgagtttgag cagcaatgtg ttcaaatgct
       61 agaacaactt ggtaaggaaa aaatcaagct tatactggaa ttatcagaaa ggaaaaaact
      121 tcaagataca aatgaagttc ataccatagt tgataaatta caaaaacata atgtcctttt
      181 tgccctcgat gattttggag tagaatattc tacatttcat tatttacagg attactctgt
      241 tgattttatt aagctggata aatctttcat ccaacagtgt gaatcaaata aaatatccga
      301 agcaatagta gatagtatag ctatgttggc aaaacgatta catattaagg tcgttgctga
      361 aggtatcgaa actatagagc aggcaaaagc catgaaaaat aaaggaatag aatatttgca
      421 agggtatctt ttttcaccac ctgtatctgg agatgttttt attcataaat ggattttgtc
      481 aaccgaaaaa aggagcaatt agatgtgtga ttcataggca tcgtttcaat tttctcagtc
      541 caagttcaag cttgtaattc tgttcctgta tatgctcttc attgggcgat agtttgcatg
      601 aacggcatca gagtatggtg ttttctcagc aagttcaaag tagcgttcca tcggagtttt
      661 accgttgtga gcgctatgag gacgttccca gttatagtaa tgctgccatt ctgcctgcag
      721 ttccttcagg tcatcagcag aaagatctac ggtagcgtaa aactcagctt tgtctgtctt
      781 ttgggaacgt tcaacctttc cgttaagatg aggcgacgct ggcttatttg gacgaaactt
      841 gatgccgtat tcttttaatc tttcctgcac tttaacggca aaaaatactc ttccccggtc
      901 tgtctgaaag cgctgtatcg ggaaaggcat ttcttcaata acacaatcaa taaaatccag
      961 tgtgtttgcc gccgtgcgac gagagtagag cctcaggact ctgtagcgag tgcaatcatc
     1021 aatagatgtg tactgatata agccggggcc aattttacag gtatccatct gaacccgatc
     1081 accggttatt gggcgctctc cgttcatgaa aatacgcata agcccggcgt caaagagcaa
     1141 atcaccgaaa tggcgttcaa cggagca
//
LOCUS       DAZGSP010000305         1125 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZGSP010000305 DAZGSP010000000
VERSION     DAZGSP010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.309"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..851
                     /locus_tag="ACHK74_005351"
     CDS             <1..851
                     /locus_tag="ACHK74_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP0223977.1"
                     /translation="QIENVFNLLDSLYVESVDTLTFKYQSIIKGLDRLFEGINLDSAF
                     SLSEEERSYFEEKAKSLNALAQLGISVEIISHELEEMDSMVTRGLNSLPTSVKEHPGF
                     SLALNAHRSLTQQIRFLSPLKISGYQSRQRITGKNIMDYVLKFFGERFERQRITIEFS
                     EEFKQIAITDIPSRIYPVFTNIINNAMYWVSLSNNRLIKIGFVNSLVIIANSGPAIDT
                     DDIPRLFELFYSKRANGHGVGLYLCRENLAVAHHKIWYSEPDEGDNYLIKDGANFVIQ
                     FNGVEF"
     gene            853..>1125
                     /locus_tag="ACHK74_005352"
     CDS             853..>1125
                     /locus_tag="ACHK74_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver domain"
                     /protein_id="HFP0223978.1"
                     /translation="MTVANYNSLVQKTFCENAIRSVVMIDDDFLTYSESIRALNNEVD
                     LDYNKIDSSKRAATLESFFQSKNMICDVDNGSVNFDVDRIRKSDLII"
BASE COUNT          370 a          178 c          211 g          366 t
ORIGIN      
        1 cacaaattga aaatgtgttt aatttgctcg atagtttata tgttgagtca gttgataccc
       61 taactttcaa atatcaatca ataataaaag gtctcgatag attatttgaa ggtataaact
      121 tagattcagc attctcatta tctgaagaag aacgctcata ttttgaagaa aaagctaaaa
      181 gtttaaacgc gcttgcacag ttaggtatta gtgttgagat aatatctcat gaacttgaag
      241 aaatggattc tatggtaacc agaggactaa actctcttcc tacttctgta aaagaacacc
      301 ctggtttttc attggcgtta aatgctcaca gatcgcttac tcaacaaata cgtttcttat
      361 cacctttgaa aatatcaggt tatcaatcca ggcagagaat aactggaaaa aatatcatgg
      421 attatgtcct gaagttcttt ggggagcgtt tcgaacggca acgaataact attgaattta
      481 gtgaagagtt taagcaaatc gcaataacag atataccatc aaggatctat cctgttttta
      541 ctaatattat caacaatgca atgtattggg tcagtctgtc aaataatagg ctcataaaga
      601 ttggttttgt gaattctttg gttatcatag caaattctgg tccggcaatt gataccgatg
      661 atatcccgcg actatttgaa ctattttata gcaaaagagc aaatggacat ggggtaggtc
      721 tgtatctatg tcgagaaaac cttgctgttg cacatcataa aatatggtat tcagaacctg
      781 atgaaggcga taactattta ataaaagatg gcgctaattt tgtgatccag ttcaatggag
      841 tggagttcta atatgacagt ggcaaattat aattctcttg tccagaaaac tttctgcgaa
      901 aatgcaattc gttccgttgt catgattgat gacgattttc tgacgtattc tgaatcaatc
      961 agggcgttga ataacgaagt tgatttagac tacaacaaaa ttgactcatc taaacgagcc
     1021 gctactcttg agagcttttt tcaatctaaa aatatgattt gtgatgttga caatggttct
     1081 gttaatttcg atgtggatcg gattagaaaa tcagatctta ttatt
//
LOCUS       DAZGSP010000306         1124 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZGSP010000306 DAZGSP010000000
VERSION     DAZGSP010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.310"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..438)
                     /locus_tag="ACHK74_005353"
                     /pseudo
     CDS             complement(1..438)
                     /locus_tag="ACHK74_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(452..637)
                     /locus_tag="ACHK74_005354"
     CDS             complement(452..637)
                     /locus_tag="ACHK74_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223979.1"
                     /translation="MFISCVPSKATPLILRAFASLLAATAVPFDGRAFAALFTVSRKP
                     RFQGCRISALKGIFTSF"
     gene            994..>1124
                     /locus_tag="ACHK74_005355"
     CDS             994..>1124
                     /locus_tag="ACHK74_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0223980.1"
                     /translation="MQLINVKRYYPKDKPYGEDVQYFQSEDGRDFYESIPLFTKKYKL
                     "
BASE COUNT          294 a          259 c          265 g          306 t
ORIGIN      
        1 gttcctgcgt gagacgacgg ggtctgccgc cggttcgtcc cctttcacgg gcagctgcaa
       61 gaccagcccg tgtccgttcg acaatcaatt cacgctccat ttctgccagc gcacccatta
      121 tgtgaaaaaa gaagcgcccc atgggggttg acgtatcaat gttgtcagta agactacgaa
      181 aatttatccc ctgttgctgt aattcatctg tcagcgcaac aaggtggcgc atactgcgcc
      241 ccagcctgtc cagtttccag accatcagcg tgtcaccggg ttttagtttc ttcagtgccc
      301 gcttcagtcc cggccgctgc gttgtttttc cgctcatttt atcttcaaaa atcagctcac
      361 atcctgcaca ttccagcgca tttctttgta acctgtgttc tgatcatttg ttgatacgcg
      421 tatatagcca ataagcatgt atttaccttc cttaaaaaga ggtgaaaatg ccttttaaag
      481 cagatatacg gcatccctga aacctcggtt tacgggatac ggtaaacagg gccgcgaatg
      541 ccctgccatc aaacggtacg gctgttgcag caagcaggct ggcaaatgcc cgcagaatca
      601 atggtgtcgc ctttgacggc acgcaggata taaacattac gtcaggaatg ccggaatcaa
      661 cggccaatca gcgttatgtt acaggggtaa gactgggagc gcaggcatta agcggtggac
      721 tggaatataa ttactccctg tcatcaggaa atgtgattac cggttttaag actgacggag
      781 actgggaaat gcgtggtggt gatgaccgtg tttattacag acagatacaa tattgtatta
      841 atgggcattg ggtatccgct gcaagtattt aattaacggg ggcatagcac acagcagcca
      901 gcatctgaaa tatgcgttat ttcagacaag cattgcctgt atatggcgat agtattattc
      961 ctgtttttat cattgattta catggtgatg tttatgcaac tgattaatgt caaacgatat
     1021 tacccgaaag acaaacccta tggtgaggat gttcagtatt tccagagtga ggacggaagg
     1081 gatttttatg aatctatccc gcttttcaca aagaaataca aact
//
LOCUS       DAZGSP010000307         1110 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZGSP010000307 DAZGSP010000000
VERSION     DAZGSP010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.311"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..264
                     /locus_tag="ACHK74_005356"
     CDS             <1..264
                     /locus_tag="ACHK74_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFP0223981.1"
                     /translation="AQITAAAEDAEIHHDYARALILWQHAYHAATLTLNKNLAVAKID
                     FCIKRIRMHEQMSRIIRKTGTDEILFRLSKHHHLYEKKKTKEG"
     gene            complement(652..1074)
                     /gene="subB"
                     /locus_tag="ACHK74_005357"
     CDS             complement(652..1074)
                     /gene="subB"
                     /locus_tag="ACHK74_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HFP0223982.1"
                     /translation="MTIKRFFVCAGVMGCLSLNPAMAEWTGDARDGMFSSVVIDQFHT
                     GQIDNNPYFCIEGKQPGGSSIRACSMKNSSVWGPSFSTLYNQALYFYTTGQLVRIYYE
                     PGVWTYPPFVKALTSNALVGLSTCATSTECFGPDRKKN"
BASE COUNT          331 a          264 c          240 g          275 t
ORIGIN      
        1 gcgcagataa cggcagcagc tgaagatgcg gaaatacatc atgattatgc ccgggcatta
       61 atattgtggc agcatgcata tcatgctgcc acgctgactc tcaacaaaaa cctggcagtt
      121 gctaaaatcg acttctgtat taaacgtatc cggatgcatg aacaaatgag caggataatc
      181 cggaagactg gcacagacga aatactcttc cgtctgagta aacaccatca tttatatgag
      241 aaaaagaaaa caaaagaggg gtgagaaacc cattgtatgg ggaaaacttg ccatgctcac
      301 tgcgggggca tttttgtact ggctgttcta accgggtaac ccgttgatac acatagtaca
      361 ataatgtttt atgcaggctt acgcgccaga ctgccatcag tagtgcattg cgtgccagct
      421 cgcggacagg ggcagtgacc agccaccaca tgagtaagca tcatttcagc gccatcaggc
      481 agctcctgaa actcctgcga gttgaaaaag catcctcctg cattagcaat actggcttgt
      541 cagtgccagt gtgatataag acgtaatgtc aaagttcgtg gtgttgacat atttctgtcg
      601 caaccaaagt atgctcccgc ccagtatata tctgggcagg agcatcattt attagttctt
      661 tttcctgtcg gggccaaaac attctgtcga tgtggcgcag gttgataacc caacaagggc
      721 attggacgtt aatgccttca caaagggcgg atacgtccat accccgggtt cataataaat
      781 cctgaccaac tgacctgttg tgtaaaaata tagtgcctga ttatataatg tggaaaacga
      841 aggcccccag acagatgaat tcttcatcga gcaggctctt atggaagaac caccaggttg
      901 tttcccttca atacaaaaat aagggttatt atctatttgc cctgtatgaa actgatcaat
      961 aacaacgctt gaaaacatgc catcccgggc atcgccagtc cactcagcca tggcaggatt
     1021 aagggataaa catcccataa cacctgcaca tacaaaaaac ctcttaatcg tcatattcac
     1081 ctccggaatt ttacagttct tcactcatcc
//
LOCUS       DAZGSP010000308         1102 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZGSP010000308 DAZGSP010000000
VERSION     DAZGSP010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.312"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(284..955)
                     /locus_tag="ACHK74_005358"
     CDS             complement(284..955)
                     /locus_tag="ACHK74_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFP0223983.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VYNSAGVITGISRVASVLWPVGQSVVEVADTEENRKADISGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKSGKLPEAFFW
                     TDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRV
                     GWGDP"
BASE COUNT          253 a          338 c          208 g          303 t
ORIGIN      
        1 ataatttagg atttatctta cattattctt ggtgtagtca ctcagaacca cgactacacc
       61 accggaacag gcatcagaaa aaactcactc tcatccggca tgctgtgtca catctgctta
      121 cactgtaatt tatgagaaaa atctcaatag cagtaaaaag taactgttca gttaaagtgt
      181 aatatttacc taagtaactt ttcctgtgta ttaccgtgaa ggagcataat tggttattgg
      241 gcgttctcct tccataaaaa tacgcagtgt cctcctttcc agattacgga tcaccccagc
      301 caaccctgta tgccagtatc tcatccgcac tggtcagctt ttccagctct ttcttcatgg
      361 tgcgctgccg gacgtggatt tccatccctt tggtgaacat cgcctgctct gccgcttcac
      421 tcagcgctat aagctcttct gctgtcactt ccacatcatt gttttccgca tccgtccaga
      481 aaaacgcctc cggcagtttc cccgatttcg ctgccgccac cgacggctca agacgcgtct
      541 gcgttgactt cccgtagtcc catttccggc cattgtgctc aaacgtgtag ttcgccgctt
      601 ccatcgcatt acgccaggcg ttaatttcat cgcccttcat ccctcgcgct ttctccgcgg
      661 tcagcaggtc cgtgattttc tccccgtcaa aaccccagcg ccccgagatg tcagctttgc
      721 ggttttcttc cgtatccgcc acctccacca cactctggcc cactggccac agaactgacg
      781 ccaccctgct gatgccggta atgactccgg cactgttata aaccaccttc agcgtctctg
      841 ctgaaaataa cgactgacac tcataccagt cctgaccatc ctctgatttc aggtacatcg
      901 cacccggcac atccggattt tccggcgtgt attgtctgaa attctttatg tgcatctgta
      961 agtcctcccg gttacatgtc tgaaagatga tgcgctttat aatggaaaat actcaaaccc
     1021 cgaagctcct gacgacacat actcacccac gctgactgta cgccactgag aaccatcaaa
     1081 tgtcacctga agcggcctgt aa
//
LOCUS       DAZGSP010000309         1093 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZGSP010000309 DAZGSP010000000
VERSION     DAZGSP010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.313"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..201
                     /locus_tag="ACHK74_005359"
     CDS             <1..201
                     /locus_tag="ACHK74_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP0223984.1"
                     /translation="SSVPNHAAIYCGNGELLHHIPEQLSKRERYSEKWQRRTHSVWRH
                     RHWSASAFTGIYNDLVAASACM"
     gene            198..737
                     /locus_tag="ACHK74_005360"
     CDS             198..737
                     /locus_tag="ACHK74_005360"
                     /inference="COORDINATES: protein motif:HMM:NF018510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0223985.1"
                     /translation="MNTAAEAIRALSLQVPGFRQKLYEGWYQLRIAGRDVSEQELHSR
                     VCEPLNDGDVIHLVPRTEGAKNGGVLQVVAGAVLAVVGYVFSWTGIGAVIGNLGVAMM
                     VGGITQMLTPRAKTPSATTTDNGKRNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQ
                     DISTRDEGGDGKVVVIGRG"
BASE COUNT          286 a          237 c          315 g          255 t
ORIGIN      
        1 tcatcggtgc cgaatcatgc cgccatttac tgtggcaacg gtgaactgct tcaccatata
       61 cctgaacaac tgagtaaacg ggagaggtat tctgaaaaat ggcaacgacg aacgcattct
      121 gtctggcgtc accgccactg gtccgcatct gccttcacgg ggatttacaa cgatttggtc
      181 gccgcatcag cctgtatgtg aacacggcag cggaggccat ccgtgccctg tcgctgcagg
      241 tgccgggatt ccggcagaaa ctgtatgagg gatggtatca gttacgcatt gcgggcaggg
      301 atgtatctga acaggagctt cattccagag tatgtgagcc gttgaatgac ggggatgtta
      361 tccatctggt gccccggacg gagggggcaa aaaacggcgg tgttcttcag gtggtggccg
      421 gtgctgttct ggctgttgtg ggatatgtgt tcagctggac cggtattggt gctgtcatcg
      481 gtaatcttgg ggtggccatg atggtggggg gtatcacgca gatgctcacc cccagggcga
      541 aaacaccgtc tgccacgaca acagataatg gtaaacggaa cacgtatttc tcctcactgg
      601 acaacatgat tgcccagggt aacccgatgc cggtgcctta cggtgaaatg ctggttggtt
      661 cacgacggat atcccaggac atcagcaccc gtgatgaggg cggagatggg aaagtggtgg
      721 ttatcgggcg gggataaaaa taaaaaaatc ccgcagagtt agcggagctg cgggaagaga
      781 tacgaagatt aactgtaagg agttattatt atgtcacagc aaaaaaaacg gtaacgcaga
      841 gaaattataa gcgccactgg gattttgtga aaatgtgaag aatttcagaa attttctgtt
      901 gcggagctga actaaaaaac gctcccgcaa tacacctgga tattaacggg aggaacgaat
      961 agtcacctta aggagttgcg attaattgct tttattgacc gcaggtaatc tacccatccg
     1021 gcaaggtttc ctccacggga atttgccgga aatgtgaaga atttcagaaa ttttattccg
     1081 tcatgacaca ggc
//
LOCUS       DAZGSP010000310         1069 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZGSP010000310 DAZGSP010000000
VERSION     DAZGSP010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.314"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..796)
                     /locus_tag="ACHK74_005361"
     CDS             complement(<1..796)
                     /locus_tag="ACHK74_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008807102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4868 domain-containing protein"
                     /protein_id="HFP0223986.1"
                     /translation="MELFAITDNTVGTRIVKIVTDRPTQNVITQLFNEQKTFFEERYT
                     EGVEFSGGYITSGDEFFVIPDFDDVIAVLDAINNPTTIPPWEPEEISVFNIIALFSGY
                     PEEDGKPATALIQSFDKRQVIDNRRTIFQKPFQASNTFCQSTEHGIVIDNKLTAILAG
                     TELKFKSFHMLRRIFDVDAYFREATNEELTSFSSHEKFSVAPGFDLTTIADSVIRKKV
                     SLINKSRILEDYSVTELRISAAEIGVVLETEKIGELEKIKMPQIRKD"
     gene            complement(805..>1069)
                     /locus_tag="ACHK74_005362"
     CDS             complement(805..>1069)
                     /locus_tag="ACHK74_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223987.1"
                     /translation="LFPLISGPDAFMDIRIASFFAVSLFFYISFSGSYSFNPLLSFWG
                     YKYYEAEDDTGVSFVILSKKPLLKASGNRVNLIKLTDYTYIAI"
BASE COUNT          337 a          193 c          178 g          361 t
ORIGIN      
        1 catcttttct tatttgcggc atttttattt tttctaattc accgattttt tcagtttcta
       61 gaacaacacc aatttcagcc gctgatattc ttaactcagt cacagagtag tcctcaagta
      121 ttcttgattt attaatcaag gaaactttct ttctaataac ggagtcagca attgttgtta
      181 aatcaaaacc cggagctact gaaaattttt catgagaaga aaaacttgtt aattcttcat
      241 tagtggcttc tctgaaataa gcatctacat caaatatcct gcggagcata tggaagcttt
      301 taaactttaa ttctgtacct gcaagaatag ctgttagctt attgtcgatg actataccat
      361 gctcagtaga ctggcaaaag gtattgcttg cctgaaatgg tttttggaaa attgttcttc
      421 tgttatcaat aacttgcctt ttatcgaagc tttggattaa ggcagttgca ggttttccat
      481 cctcttcagg atagcctgaa aacagagcga ttatattgaa gactgatatt tcttccggct
      541 cccatggagg gatagttgtg ggattattaa tagcatctaa tacagcgata acatcatcaa
      601 agtcaggaat gacaaaaaac tcatctccac tagtgatgta gccacctgaa aattctacgc
      661 cttctgtata gcgttcttca aaaaaagttt tttgttcatt gaatagctgt gtgattacat
      721 tttgagtcgg cctgtcagta acaattttta caatgcgtgt gccaactgta ttatctgtta
      781 ttgcaaacaa ttccatattt attcctatat tgctatatat gtgtaatcag taagcttaat
      841 caaattaaca cgattgcctg atgcttttaa tagtggcttt ttcgataata taacaaagct
      901 tacgcctgta tcgtcttcgg cttcataata tttatatccc caaaatgata ataatgggtt
      961 gaaactgtag gaacctgaaa aactgatgta aaaaaataaa ctcacagcaa agaaagaagc
     1021 aatcctaata tccataaatg catctggacc actgattaag ggaaataaa
//
LOCUS       DAZGSP010000311         1066 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZGSP010000311 DAZGSP010000000
VERSION     DAZGSP010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.315"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            229..963
                     /locus_tag="ACHK74_005363"
     CDS             229..963
                     /locus_tag="ACHK74_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HFP0223988.1"
                     /translation="MSDNTIPEYLQPALAQLEKARTAHLENARLMDETVTAIERAEQE
                     KNALAQADGNDADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACDSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSILVQENPLAN
                     TTGHQGYVAPEKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQGAELKARGLLS"
     gene            960..>1066
                     /locus_tag="ACHK74_005364"
     CDS             960..>1066
                     /locus_tag="ACHK74_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFP0223989.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCS"
BASE COUNT          275 a          279 c          295 g          217 t
ORIGIN      
        1 ttcacccttt aacttactgt attaattatc ttttttatta aggtgaacag aggtgaacag
       61 taaaatataa aaaaacaaac agtaagccgg tttttcctgc gaccttttcc tggcttgccg
      121 gtgtgaggat gagtctcctg tgtcagggct ggcacatctg caatgcgtcg tgttgttgtc
      181 cggtgtacgt cacaattttc ttaacctgaa gtgacgagga tccggaaaat gtctgacaac
      241 accatccctg aatatctgca acccgcgctg gcacaactgg aaaaggccag aaccgcccat
      301 cttgagaacg cccgcctgat ggatgagacc gtcacggcca ttgaacgggc agagcaggaa
      361 aaaaatgcgc tggcgcaggc cgacggaaac gacgctgacg actggcgcac ggcctttcgt
      421 gcagccggtg gtgtcctgag cgacgagctg aaacagcgcc acattgagcg cgtggcacgc
      481 cgggagctgg tacaggaata tgacaatctg gccgtggtgc tgaattttga acgcgaacgc
      541 ctgaaagggg cgtgtgacag cacggccacc gcctaccgga aggcacatca tcaccttctg
      601 agtctgtatg cagagcatga gctggaacac gccctgaatg aaacctgtga ggcgcttgtc
      661 cgggcaatgc atctgagcat tctggtacag gaaaatccgc tcgccaacac caccggccat
      721 cagggctacg tcgcaccgga aaaggctgtc atgcagcagg tgaaatcatc gctggaacag
      781 aaaattaaac agatgcaaat cagcctcact ggcgagccgg ttctccggct gaccggactg
      841 tcagcggcaa cactcccgca catggattat gaggtggcag gcacaccggc acagcgcaag
      901 gtgtggcagg acaaaataga ccagcagggt gcagagctta aggccagagg actgctgtca
      961 tgatttactg cccgtcgtgt ggacatgttg ctcacacccg ccgcgcacat ttcatggacg
     1021 atggcaccaa gataatgatt gcacagtgcc ggaatattta ttgctc
//
LOCUS       DAZGSP010000312         1056 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZGSP010000312 DAZGSP010000000
VERSION     DAZGSP010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.316"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..391)
                     /locus_tag="ACHK74_005365"
     CDS             complement(<1..391)
                     /locus_tag="ACHK74_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFP0223990.1"
                     /translation="MQIIIMTRDRYLEYGLMRMLNGYRLTTGRELFDAGKRRLPLPED
                     SYVILCDRNLERLTYCMFCGRRFLVIPVSSVRCLTDIRQAIRRGAWLFGHTARPLTRT
                     EMVVVFGVIFHEYGCTFLADQLGISMKT"
     gene            complement(454..660)
                     /locus_tag="ACHK74_005366"
     CDS             complement(454..660)
                     /locus_tag="ACHK74_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223991.1"
                     /translation="MLNYRISSVQVFTVNGRADRVQVRLECDDAAVFECCLLAEGEGE
                     LKELSLSELEERALMYAADSFRYE"
     gene            complement(788..>1056)
                     /locus_tag="ACHK74_005367"
     CDS             complement(788..>1056)
                     /locus_tag="ACHK74_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFP0223992.1"
                     /translation="RMNSQQRQINENHEEMKRAAAQSAALAGLFQPYSVGKFNATAAV
                     GGYSDRQAVAVGMGYRFNEQAAAKAGTAFSDGDVSWNVGVNWEF"
BASE COUNT          269 a          340 c          218 g          229 t
ORIGIN      
        1 ccgtcttcat gcttatcccc agctggtctg ccagaaaggt acagccgtac tcatggaaaa
       61 taaccccgaa gaccaccacc atctcggtac gggtcagtgg tcttgccgta tgtccgaaca
      121 gccacgcacc acgacggatg gcctgcctga tatctgtcag gcatctcaca gaggaaacag
      181 gaatgacaag aaaacgacgc ccacagaaca tacagtatgt aagcctttcc agattacggt
      241 cacagagaat cacataactg tcttcgggaa gtggcagacg gcgctttccg gcatcaaaca
      301 gttcacggcc tgtcgtgagc ctgtatccgt tcagcatacg catcaggcca tattccagat
      361 atctgtcacg ggtcataata ataatctgca tactctcaga cccttatctc ccggataccg
      421 gctccgttca ccattaccgg tataactgac tcatcattca taacggaaac tgtctgccgc
      481 atacatcagt gcccgctctt ccagctctga cagactcagc tctttcagtt ccccttctcc
      541 ttcagcaaga agacaacatt caaaaacagc tgcatcgtcg cattccagac gaacctgcac
      601 cctgtcagcc cgaccgttta cagtaaatac ctgcactgaa cttatgcgat agttcagcat
      661 atacactctc cctctcagct cctcaggaat accccggaac cgtcagtcat gccaccgggg
      721 agaaaagcag gcagcgaatc atgaccgcat acagaacgct gcccgccccg gtgactcaga
      781 cccgggttca gaactcccag ttgacaccca cattccagga aacatcaccg tcactgaacg
      841 cagtacctgc ctttgccgca gcctgctcgt taaagcgata tcccatgccc accgcaacag
      901 cctgcctgtc actgtacccg cccacagccg ctgtggcgtt aaatttaccc acactgtatg
      961 gctggaaaag accggccagc gcagctgact gggctgctgc ccgcttcatt tcctcatgat
     1021 tctcattaat ctgacgctgc tgactgttca tacgac
//
LOCUS       DAZGSP010000313         1049 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZGSP010000313 DAZGSP010000000
VERSION     DAZGSP010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.317"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1049
                     /locus_tag="ACHK74_005368"
     CDS             <1..>1049
                     /locus_tag="ACHK74_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin"
                     /protein_id="HFP0223993.1"
                     /translation="KEDDTEIRIEGFWEECQPGETQCTAVFNVVGENGQVTEAVLTQD
                     GPVLENFTAGQSGTLSDDDRGGFIWLGGLAFGGGLAAMALAAGGGGGSKHRHENHEAD
                     TTAPSAPALTAGDNGSVSVELPDDANKGDTVEITFVNENGDGQKVTLEKGDNGWTSDN
                     SALIPDSQGDTATIAPDTVKDNSEVTAVAKDPAGNESAPVTVTSKTDGVSDAPVLTIP
                     EAADSVNAEELKDGVQAEVTLPAGTVEGAVITLTVTHPDQSTENVTHNVTGDEVTAGK
                     VSMDIPEDAVVDGQNSVSVSLTQGSNPAKAGNTVEIVVDGQVPGDTNGDGVADTTPVV
                     TIPEATGGVNAKELK"
BASE COUNT          287 a          217 c          328 g          217 t
ORIGIN      
        1 aaggaagatg atacagaaat ccggattgag ggattctggg aagaatgcca gccgggtgaa
       61 acacagtgta cagcagtatt taatgttgtg ggtgaaaatg gtcaggttac tgaagctgtc
      121 ctgacccagg atggtcctgt actggagaat ttcactgcag gacaatccgg gaccttgtca
      181 gatgatgatc gtggcggttt tatatggttg ggcggtctgg catttggtgg ggggcttgct
      241 gctatggctt tggctgcagg tggtggtggt ggcagcaagc acagacatga aaaccatgag
      301 gcagatacaa cagcaccatc agcaccggca ttaacggccg gagataatgg ttcagtttct
      361 gttgaactgc cggatgatgc gaataaaggc gatactgttg aaatcacctt tgtaaatgaa
      421 aatggtgatg gacagaaagt cacactggag aaaggggaca acggctggac atccgacaat
      481 tctgcactga tcccggacag ccagggtgat acagccacaa tcgccccaga cactgttaaa
      541 gataacagtg aagtcacagc agttgctaaa gatccggctg gtaatgaaag tgctccggtc
      601 actgtgacgt caaaaactga cggtgtgtcg gatgctcctg tcctgaccat tccggaagcg
      661 gcggacagtg tgaatgccga agaactgaag gatggcgtac aggcagaagt cacgttgccg
      721 gcaggaacag tggaaggtgc ggtcattaca ctgacagtga ctcatccgga tcaaagcacc
      781 gaaaacgtga cacataacgt caccggagat gaggttactg ccggtaaggt atccatggat
      841 attcctgagg atgctgttgt tgacggtcag aacagtgtga gtgtcagcct gactcagggc
      901 agtaacccgg ctaaagcggg gaataccgtg gagattgtgg tcgacggtca ggttccgggt
      961 gacaccaacg gtgacggagt ggcggatacg acaccggtag tgaccattcc ggaagcgacg
     1021 ggcggagtga atgccaaaga actgaagga
//
LOCUS       DAZGSP010000314         1048 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZGSP010000314 DAZGSP010000000
VERSION     DAZGSP010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.318"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..414)
                     /locus_tag="ACHK74_005369"
     CDS             complement(<1..414)
                     /locus_tag="ACHK74_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFP0223994.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMLS
                     FFGQYKTLIEDLIEHFRYGNGSNFHSQQLNLSFHEK"
     gene            595..747
                     /locus_tag="ACHK74_005370"
                     /pseudo
     CDS             595..747
                     /locus_tag="ACHK74_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(772..>1048)
                     /locus_tag="ACHK74_005371"
     CDS             complement(772..>1048)
                     /locus_tag="ACHK74_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223995.1"
                     /translation="NDINLALSFILVINNTYVNINIGAKRIRDTGCKAIYFVLISLGL
                     TLFSIISTAIEGVRCESLSVQLNVAWILFTTIMLSAIPSAKMSLSAH"
BASE COUNT          337 a          180 c          192 g          339 t
ORIGIN      
        1 tttttcatga aaagataaat ttaattgttg gctatgaaaa ttgctgccat ttccatatct
       61 aaaatgttca atcagatctt cgataagtgt tttatattgt ccaaaaaagg aaagcatttt
      121 tgctagttct ttcatttctg caaataaaat atcagtgcat tcacgttgtg atatttttgt
      181 tccggaactt gaggcactaa aagcaacatt aatagaacct gggccaggga aatggtattt
      241 aataagtctg taaggatcaa cttttgctga gatatcgctt aggccgagtg agagaaagtc
      301 ttgttctacc atatcaccat attgcatatc atcagcagaa aaatcatcga ataaactaat
      361 agtttcgaaa agtgtacagg gaatattaat tggcggaagt aatatgttat tcatttgcat
      421 ttgccttggt tatatttaag ctcaatatca attcgagaac aaatgctagg gtaaaaaaat
      481 atttaaaaaa gaagtaaact tcacattttt tcattacatc tctgaagaga acctcgttaa
      541 tgattggtct tgctcattgt taataacaaa atgttgaatt taccgattag gaaacttttt
      601 cctacgcgga ttgcctccag ctatggcatg aactttgagt ttatgccaga actgaagtgg
      661 ggcttcggct accctggcgc gattatcttt atgatcctcg cgggcctgac atcgtatctg
      721 tactttaagc gtaagaactg gctgtaacta tacgatgtcg ctgtgagtaa gttagtgtgc
      781 tgacagcgac attttagccg agggaatcgc agataacata attgttgtga ataatatcca
      841 tgccacgttg agttggacgc ttaatgactc acatctcacc ccttcaattg ctgtagagat
      901 aatagaaaaa agtgtcaaac ccaatgaaat caatacaaag tagattgcct tacatccagt
      961 atctcttatt ctttttgctc caatattgat atttacatag gtattattaa taaccagaat
     1021 aaaactcaaa gccaggttga tatcattc
//
LOCUS       DAZGSP010000315         1046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZGSP010000315 DAZGSP010000000
VERSION     DAZGSP010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.319"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..201
                     /locus_tag="ACHK74_005372"
     CDS             <1..201
                     /locus_tag="ACHK74_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP0223996.1"
                     /translation="ASVPNHAAIYCGNGELLHHIPEQLSKRERYSEKWQRRTHSVWRH
                     RHWSASAFTGIYNDLVAASACM"
     gene            198..779
                     /locus_tag="ACHK74_005373"
     CDS             198..779
                     /locus_tag="ACHK74_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0223997.1"
                     /translation="MNTAAEAIRALSLQVPGFRCQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEPLSGRAVIHIVPRLAGAGGNGVFQVVLGVAAIVGSFFTGGATLAAWGAALSAGGL
                     TATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPV
                     PYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          247 a          236 c          337 g          226 t
ORIGIN      
        1 gcatcggtgc cgaatcatgc cgccatttac tgtggcaacg gtgaactgct tcaccatata
       61 cctgaacaac tgagtaaacg ggagaggtat tcagagaaat ggcaacgacg aacgcattct
      121 gtctggcgtc accgccactg gtccgcatct gccttcacgg ggatttacaa cgatttggtc
      181 gccgcatcag cctgtatgtg aacacggcag cggaggccat ccgtgccctg tcgctgcagg
      241 tgccgggatt ccgctgtcag atgaacgaag gctggtatca gatacgtatt gccggtgagg
      301 ataccgcgcc ggaggcggtg tatgcccgtc ttcatgagcc actgagcggg agggccgtga
      361 ttcatattgt accgcggctg gcaggggccg ggggaaatgg tgtttttcag gttgtgctgg
      421 gggttgcagc gattgtcggg tcatttttca ctggtggtgc aacactggcg gcatggggcg
      481 cagccctgag tgccggaggg ctgactgcca ccacgatgct gttctcactg ggggccagca
      541 tgattctggg gggtgtggcc cagatgctgg ccccgaaggc aaagacgccg gaatacaaaa
      601 gtacggataa cggtaaacag aacacgtatt tttcgtcact ggacaacatg attgctcagg
      661 gtaacccgat gccggtgcct tacggtgaaa tgctggttgg ttcacgacgg atatcccagg
      721 acatcagcac ccgtgatgag ggtggtgacg ggaaagtggt ggttatcggg cggggatgaa
      781 aataaaaaaa tcccgcagag ttagcggagc tgcgggagag aacgatgaag attaacttta
      841 tggagttatt tttcaggcat caaaaaagta acgcagcgtc attattgcgg ctacaggcaa
      901 ttgccggaaa tgtgaagagt ttcagaaatt ttattccgtc atgacacagg caccctccgg
      961 ggtgcctgtt gtttttgggc ataaacagat tcagacatca gacaggagag ggggacagag
     1021 tgggtaaagg gggcggcaag gggcac
//
LOCUS       DAZGSP010000316         1026 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZGSP010000316 DAZGSP010000000
VERSION     DAZGSP010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.320"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..515
                     /locus_tag="ACHK74_005374"
                     /pseudo
     CDS             1..515
                     /locus_tag="ACHK74_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
     gene            544..729
                     /locus_tag="ACHK74_005375"
     CDS             544..729
                     /locus_tag="ACHK74_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223998.1"
                     /translation="MVSLKESILLETSAKIQSIEVKREEKFIQGYYDGYTKGIIDVMD
                     NFIPLISLLSSELEKKE"
     gene            732..>1026
                     /locus_tag="ACHK74_005376"
     CDS             732..>1026
                     /locus_tag="ACHK74_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001701978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0223999.1"
                     /translation="MINDLKSILLKSSEEVDVFIKIFESWRKKLPAISGPVNLYIPTR
                     FKDKYLEVESYFVDKSIWNVHITFHDDKRFVFFTDQFIAEFSPQEFVDNCEQYL"
BASE COUNT          355 a          152 c          176 g          343 t
ORIGIN      
        1 cttgaataat acacctatta acaatccggt gcttagatct attaccaatg aaatgattgt
       61 tctgcagtac aatctttcag ttgaacattt taatctgaat agttcactta tttactacat
      121 aaacaattgg aatttatttc cattgttctg tttattcagt ggttatcact tttatcgaga
      181 aaggtttgca gaacgcggat tttttataag gttcctgctg tgctaaggga ttatttatca
      241 gctataccgg tgaaaataaa tgaaaaagca cgatacaaac ccggcatcgc tagctatcac
      301 aatatcatca catgtggttt ttcaacgttg tcaccctata taaggcaaca accattggca
      361 atgcagcaac gatttaattt attatttcct gattttgttg atcacataca attgccgtta
      421 ccacttgcat caactctttt agagagaatt acattttatg cgaaaaaaat agagatgagc
      481 ttgataaaat ctcctgcaaa tggtgttgtg attaaaagaa aaatttctga tggattgaaa
      541 taaatagttt ctcttaaaga aagtatcctt cttgaaacca gcgcaaaaat tcagagcatt
      601 gaggtaaaga gagaagaaaa atttattcaa ggttattatg atggctatac aaaaggcatt
      661 attgatgtga tggataattt tatacccttg ataagcttgt tgagctcaga actagaaaaa
      721 aaagaataaa catgataaac gatctgaaga gtatattgtt aaaatcctca gaggaagttg
      781 atgtttttat caaaattttt gaaagttgga gaaaaaaatt accggcaata tcaggtcctg
      841 taaatctcta tatacctacc agattcaaag ataagtatct tgaagtagag tcatattttg
      901 tcgacaaatc aatatggaat gtccatatta catttcatga tgataagcgt tttgtgtttt
      961 ttaccgatca atttattgct gaatttagtc cgcaggaatt tgtggataat tgtgagcaat
     1021 atttaa
//
LOCUS       DAZGSP010000317          994 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZGSP010000317 DAZGSP010000000
VERSION     DAZGSP010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.321"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            130..366
                     /locus_tag="ACHK74_005377"
     CDS             130..366
                     /locus_tag="ACHK74_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224000.1"
                     /translation="MIKDNSKVDDSADRLSFTETLFVAHSILPDLTQTKTKENDCYLL
                     FKKKCHIVHIKSRGILRYVTDAIVSQDLMVVAHV"
     gene            434..679
                     /locus_tag="ACHK74_005378"
     CDS             434..679
                     /locus_tag="ACHK74_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224001.1"
                     /translation="MNCDNNHRNEEFIVTFDKGNKQDNSRRKHDNFPIEVESSVEQET
                     HCITNNKSASGIVTHDYDADYICGCGEIMCPGCGHDL"
BASE COUNT          344 a          175 c          183 g          292 t
ORIGIN      
        1 ggacagtttt ttataactca ttaatcccta cctgttgctt tattttcatt atgaaaagtt
       61 accaaatctt agcacctcag tcaggagagt caggtcatcg ataaagaata ttccagtaga
      121 gtggcgctca tgatcaaaga taacagcaaa gttgatgaca gcgctgaccg actgtcattt
      181 acagaaaccc tgtttgtagc acattccatc ttacctgacc tcacccagac aaagacaaaa
      241 gagaatgatt gttatttact tttcaaaaaa aagtgtcata ttgtccatat aaagtcaaga
      301 ggtattttac gttatgtcac tgatgcaatt gttagtcagg atctcatggt tgtcgctcat
      361 gtataacctg agacttgaac gcacactaat agcactgacg gtcatacatc gggaaatgtg
      421 aggaggcata agaatgaatt gtgataataa tcacagaaat gaagaattca ttgttacctt
      481 tgataaaggc aacaagcaag acaattcaag acgaaaacac gataattttc ctatagaggt
      541 agaatcctcc gtagagcagg agacacactg tatcacaaat aataagtcgg cttccggtat
      601 agtaacacat gactatgatg ccgattatat ttgtggttgt ggtgaaatta tgtgtcctgg
      661 ttgcggtcat gacctataac aaacattttt gcaaactgat acctgatcag gtacaagcct
      721 gttgattatt atgctgaata tttcatgtta taaaaccatc gatataaaca ttaacaagaa
      781 aaaacatata ttgtttatct gaacatttat cgatggaaaa ccaaagggga aagtatgtat
      841 cttacgaaaa agattataat aagtatgatg tttatattac catctgctgc attttcatca
      901 gatccacctc cccttcaaca atcgttagaa aaaacaacct atttttctat aggtatgaat
      961 gggtttatag gctatcagag cgaaggggaa aaat
//
LOCUS       DAZGSP010000318          994 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZGSP010000318 DAZGSP010000000
VERSION     DAZGSP010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.322"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..929
                     /locus_tag="ACHK74_005379"
     CDS             <1..929
                     /locus_tag="ACHK74_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP0224002.1"
                     /translation="DRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNG
                     DPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIH
                     AQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVG
                     SVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNN
                     VTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVN
                     GDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
BASE COUNT          278 a          225 c          288 g          203 t
ORIGIN      
        1 gggaccgtta taacccggca gaccaagaca gttcgtgctg gatccgtgtg gcacaggcgt
       61 gggcaggcac cggttttggc cacctggcga taccgcgtgt gggtcaggag gtgattgtgg
      121 acttcctcaa cggcgatccg gaccagccga ttattatggg gcgtacctac caccaggaaa
      181 accgcacacc cggcagcctg ccggggacga agacgcagat gaccattcgt tcgaaaacct
      241 ataagggcag cgggtttaat gaactgaagt ttgacgatgc gacagggaaa gaacaggtct
      301 acatccacgc gcagaagaac atgaacaccg aggtgctgaa taaccgcacc acggatgtga
      361 taaacaacca tgctgagacc attggcaaca atcagatgat tgcggttacc aacaatcaga
      421 tacagacggt gggcgttaac cagatagaga cggtgggcag taaccagatc attaacgtgg
      481 gttctgttca ggtggaaacg attggacttg ttcgtgcgct gaccgtgggc gtggcgtacc
      541 agacgacggt aggtggcatt atgaacactt cggtggctct gatgcagtcc tcgcagatcg
      601 gtttgcataa atcgttgagg gtcgggctgg gttatgacgt caaagtcgga aataacgtta
      661 ccttcaccgt tggtaaaacg aaaaaggatg ataccgggca gaccgcgatt tactccgccg
      721 gtgagcatct ggagctctgc tgtggtaagg caaggctggt gctgacgaag gacggacaaa
      781 tttttctcaa cggcacaaaa attcatttgc agggtaagga gcaggttaat ggtgactcac
      841 tgttgattaa ctggaactgt gcagcctcga aatccccacc gaagacccct gatgaaaagc
      901 aggatacgcc ggatatgaga gagtactgat cccgtacctg ttcggtattc agtaacaaac
      961 aagcattaaa aataaacaga agaaggaatt gaat
//
LOCUS       DAZGSP010000319          985 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZGSP010000319 DAZGSP010000000
VERSION     DAZGSP010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.323"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(55..879)
                     /locus_tag="ACHK74_005380"
     CDS             complement(55..879)
                     /locus_tag="ACHK74_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224003.1"
                     /translation="MHRSSNLLFLSLMLFSVTGCSFNQLPGTIISRSPDKKLSPPGDT
                     KWVNIEIKNPSPYTKPFPLSVRYISYECQKKRISGFDGSVVSEPGYNVKEIPMQQERG
                     DIWKAKVAMTGGGSCNWTLSAVTLGIEYTDATHLGKGLVPGSAVGVRLAFDGYASRNG
                     QFDFIGGNQFDYYPKYYPFIKKWSDSSQGLMHDKLYLFTEEESIRKIKINFNKNEDVF
                     VNYRPLIDESKKVVMTFPTQKGSGAKYTFEYPDGSVVKSKNINPDFKKIEAISSKR"
BASE COUNT          307 a          223 c          143 g          312 t
ORIGIN      
        1 actccccccc tccgtatact gaaaaaacac atcatcaaaa atgcaaaaaa acattcatct
       61 cttacttgaa atcgcctcta tttttttaaa gtcaggattt atattttttg atttcacaac
      121 actcccatca ggatattcga aagtgtactt cgccccactc cctttttgag ttgggaatgt
      181 cattaccact ttcttacttt catcaattaa aggtcgataa ttcacaaaaa catcttcatt
      241 tttattaaaa tttattttta tctttcgaat actttcttct tccgtaaata gatacagttt
      301 atcatgcatt aaaccctgtg aactatcgct ccatttcttt atgaacggat agtatttagg
      361 atagtaatca aactgattcc caccaataaa atcaaattgc ccatttcgtg aagcataacc
      421 atcaaaagcg agcctcacac caactgcact acccggcaca agacctttac caagatgagt
      481 ggcatcggta tattcaatac ctagcgtaac ggcacttaat gtccaattgc atgaaccacc
      541 accagtcatc gctactttcg ctttccagat atctcctctc tcttgctgca tcggaatttc
      601 cttaacatta tagccgggtt ccgaaacaac agagccatca aatccactta ttcgcttttt
      661 ctgacattca taagagatat atctgacgct taacgggaaa ggttttgtat aaggtgatgg
      721 attttttatt tcgatattaa cccattttgt atctcccgga ggtgatagct ttttatcagg
      781 cgaccggcta attattgtac cgggaagttg attaaaacta caccctgtaa cagaaaataa
      841 catcaaggaa agaaacagca agtttgagct tctatgcatt ttccattacc tcttcactat
      901 acttagcaaa tcgtaaaagt gtatcgttac cggaaggcat ggataatgta gatgaaagag
      961 atacatccaa catgctttca atatc
//
LOCUS       DAZGSP010000320          980 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZGSP010000320 DAZGSP010000000
VERSION     DAZGSP010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.324"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(753..>980)
                     /locus_tag="ACHK74_005381"
     CDS             complement(753..>980)
                     /locus_tag="ACHK74_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0224004.1"
                     /translation="LQEEKVSFSQILLDIRMHNARQLISSKLSVNQIAIRCGYASTPY
                     FISVFREYFGITPHQMMINQSGFIESRIAIK"
BASE COUNT          309 a          197 c          191 g          283 t
ORIGIN      
        1 ttccgcatgg tgatgattta tacacctgca ctgaattttt ctgtatctcc agagctgaat
       61 cgataaacag aagtggtaat aatgaaagtt aatcacataa aaaagaggtt accgccttat
      121 aatgataaga gttcattatc cattccatcc agacaaaacc tgcctgtgta taaaaaataa
      181 gaaccatgtc acaaaaacat tgcattccgg caagttatga cagaattact gaaaaccgtt
      241 atctgtagtt attgtagttt ctgatatatg ctcatccacc cggaaatacg acatggtcct
      301 gactggcgtt cagttcaaag tgtaatttat tattcacaca ctgagactga aaaacggatg
      361 gcgtggatga gtacacagcc tgaagagtat aaaaggaaca gctccggatt gttacccggg
      421 attactatag ttcaccacac aaccgtaaac ctggttgtgt ggtgaaacag taacatatta
      481 agaatattgt gttaccacct gttttctcag gaaacggatt gcaggagagg tatactcatt
      541 catcttcccg gagttttgtt aatcactgca taatcaaacg gaccaataca ttctttgctc
      601 gcatccgtaa aaatgtcgcc acaatatcat cagtcagtca actcattacc atctgttttc
      661 cggggccgta gttactacag tgctccggag tgataaacag aaactgataa tgaagcataa
      721 gaatactttc attatgcgct gactgacata ctttacttaa ttgcgattct gctctcaata
      781 aatccggact gattaatcat catctgatga ggtgttatgc caaaatattc tctgaaaacg
      841 ctgataaaat acggtgtgct ggcataaccg caccgtattg cgatttgatt cactgacagt
      901 ttactggaaa taagttgtcg ggcgttatgc atgcgtatat ccagcaggat ttgggagaaa
      961 gaaacttttt cctcctgaag
//
LOCUS       DAZGSP010000321          979 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZGSP010000321 DAZGSP010000000
VERSION     DAZGSP010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.325"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            173..418
                     /locus_tag="ACHK74_005382"
     CDS             173..418
                     /locus_tag="ACHK74_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013184986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0224005.1"
                     /translation="MSLIEDWSSADIKAALEKRDTNLSRLSRENNLQPRTLGNALRVP
                     YPKGEKIIARAIGVEPQVIWPTRYERRARRKYVGCND"
     gene            399..>979
                     /locus_tag="ACHK74_005383"
     CDS             399..>979
                     /locus_tag="ACHK74_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0224006.1"
                     /translation="MWVAMTELISFSDLPSSLAGLHKKAKREAWKTRLKPGVKGKVLE
                     CEIGALPLTVQQAVRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQLM
                     VERLNALTENQRQVADARIAIVSEVLKVAQQPGFSCAKAIRFIVDNLARSQLDERIVA
                     MVETANAKKGNSRALSEITLKRWIAAFNKAQNAA"
BASE COUNT          305 a          202 c          251 g          221 t
ORIGIN      
        1 aataactcaa taaaaaacaa atagatacag agcaaattta aaaatttgaa attaaccagt
       61 tgatatttac taagttaata tctaacatat caaacataca ctagtgagta actcaataga
      121 gtagcacaca tgagtaataa agtttgccat agaaagtaca cagggggcca ttgtgagtct
      181 tattgaagat tggtcttccg ccgatataaa ggcagcctta gaaaaacgcg ataccaattt
      241 gtcacgttta tcaagggaaa ataatttgca gcctaggact ttgggtaatg ctttgcgagt
      301 tccctaccct aaaggtgaaa agataattgc tagggctata ggtgtagagc cacaggttat
      361 ttggcctact agatatgaac gacgtgcgcg gaggaaatat gtgggttgca atgactgaat
      421 tgatttcatt cagtgaccta cctagttctt tggcaggtct gcacaaaaaa gcaaagaggg
      481 aagcgtggaa aacgcggcta aaaccagggg taaaagggaa agttttagag tgtgaaatag
      541 gagctttgcc tttaactgtt cagcaggcag tacgcgaacg ctatgccctg caactgatga
      601 cgcaaaaagc cgatgaatca ccggctccgg tagtgacaaa ggccaggcgc tcacctgccg
      661 tggttgatgc ggtggaggca tatcgcggat caccacaact gatggtcgaa cgccttaatg
      721 ccctgactga aaaccagcgc caggtggctg atgcacgaat cgcgatcgtc agcgaagttc
      781 tgaaagtcgc gcaacaacct ggtttcagct gtgcgaaggc tatccggttt atcgttgaca
      841 acctggcacg ttcgcagctg gacgagcgca ttgtggcaat ggttgagacg gcgaacgcca
      901 aaaagggaaa cagccgcgcg ttaagtgaaa tcacgctgaa acgctggatt gcggccttta
      961 acaaggcgca gaacgccgc
//
LOCUS       DAZGSP010000322          962 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZGSP010000322 DAZGSP010000000
VERSION     DAZGSP010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.326"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..720
                     /locus_tag="ACHK74_005384"
     CDS             <1..720
                     /locus_tag="ACHK74_005384"
                     /inference="COORDINATES: protein motif:HMM:NF014452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFP0224007.1"
                     /translation="MTVSLVLSVTLSALPGKASTVSAEIPYQTFRDFAENKGVFTPGV
                     TGIEIKDNNGNAVGTLDVPMIDFSSVSRRGSLTLLSQGYGVSAKHGDLGDVNNASFGY
                     DKNNYTVVKNNKHSGLDFSLHRFSKLIAEATPADINISGQLSDSSQYTAFYRAGAGTQ
                     YIKERSGKQTHIPGTFLTGGTVGTPWYSGNNLISSSPGDTYNKSQGPLASYGQMGDSG
                     SPLFAYDSLSEKMVSGWSHPA"
     gene            683..>962
                     /locus_tag="ACHK74_005385"
     CDS             683..>962
                     /locus_tag="ACHK74_005385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224008.1"
                     /translation="MKKWSLAGVTLHNYGVNGYRNNWLLLPEDYIRNIIVADFDPIIS
                     FNKNSKEHMSWTYDAAKGVGRIQQDDQQFVMHGNLNGNLNAGKNLYFTG"
BASE COUNT          293 a          183 c          218 g          268 t
ORIGIN      
        1 atgacggttt ccctggtatt atcagtgact ctttctgctt taccgggtaa agcatcaacg
       61 gtcagtgcag aaataccata tcagactttt cgtgactttg ctgaaaataa aggtgtgttt
      121 acccccggag tgacaggaat tgaaataaaa gataataatg gaaatgcagt tggtacgctt
      181 gatgtgccaa tgattgactt cagcagcgtg tcacggcggg gctcactgac cctgttgtcg
      241 cagggatatg gtgtatcagc aaaacacgga gaccttggtg atgtaaacaa tgccagcttt
      301 ggctatgata aaaataatta tactgtagta aagaacaaca aacattcggg ccttgatttt
      361 tctctgcatc ggttcagcaa actcatcgca gaggctacac ctgctgatat aaatatatct
      421 ggacaactta gtgattcgtc acagtatact gctttttaca gagcaggcgc tggcacacag
      481 tatattaaag aacgctcagg taaacagact catatcccgg gaacattttt aactggtgga
      541 acagtaggca ctccatggta ttcaggaaat aatttaatat caagctctcc aggtgacacc
      601 tataataaat cacagggacc attagccagt tatgggcaga tgggtgacag cgggtcgcca
      661 ttatttgctt atgattctct cagtgaaaaa atggtctctg gctggagtca ccctgcataa
      721 ctatggggtg aacggttaca gaaataactg gttgttatta cctgaggatt atatcagaaa
      781 tattattgta gccgatttcg atcccattat ttcatttaat aaaaacagta aagaacatat
      841 gtcatggaca tatgatgcag ccaaaggcgt gggccgtatt cagcaggatg accaacagtt
      901 tgtgatgcat ggtaacctga atggtaatct gaatgcaggt aaaaatcttt attttacagg
      961 cg
//
LOCUS       DAZGSP010000323          958 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZGSP010000323 DAZGSP010000000
VERSION     DAZGSP010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.327"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            22..958
                     /locus_tag="ACHK74_005386"
                     /pseudo
     CDS             22..958
                     /locus_tag="ACHK74_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          335 a          180 c          183 g          260 t
ORIGIN      
        1 tccactattg ctgggtaaga tcaagataac atctctaagg atgaatggga aaacctccat
       61 gcattaaaag aaaagcgttt aaatggaatg cctaaaacaa cacccaaaag tgaccaggtt
      121 attatgttgc aaaatcaata cgttcgtgaa atgcgaaaat atggcgtacg aggtttacgt
      181 tatgatgcgg caaaacactc aaaacatgaa caaatagaga ggtcgataac cccaccgctt
      241 aaaaattata atgagcggtt acacaatact aatttattta acccaaaata tcaaaaaaaa
      301 gccgttatga attacatgga atacctggta acttgtcagt tggatgaaca acaaatgtca
      361 tcactgcttt atgagagaga tgatttaagt gccattgatt tttctttact catgaaaacg
      421 ataaaagcct tttcatttgg tggagatctc caaacccttg catcaaaacc gggttccaca
      481 atctcaagta tcccatcaga aagacggata ttgattaaca ttaaccacga ttttcctaac
      541 aatggtaatc ttttcaatga ctttctattt aaccatcaac aagatgaaca attagcaatg
      601 gcatatatgg ccgctctccc gttcagcaga cctttagttt acagggatgg ccaggtatta
      661 aaatcaacga ctgaaattaa aaattatgat gggtccacgc gtgtcggcgg tgaggcgtgg
      721 cttaataaag gttgctctac ctatcagcag ctctacaatg aattccacgc attatatata
      781 gataaagcag gaatatggag cgcatttgag ggtgtatttg caactaaaaa cgttctggcc
      841 tttagtcgtg gggattctgt gaacattaat cactctcctc atgatggact agttataata
      901 aataaaggaa acgaagaagt tgaaggtacc tggcctaaca aattgcaacc tggaatat
//
LOCUS       DAZGSP010000324          956 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZGSP010000324 DAZGSP010000000
VERSION     DAZGSP010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.328"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            130..>956
                     /locus_tag="ACHK74_005387"
     CDS             130..>956
                     /locus_tag="ACHK74_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HFP0224009.1"
                     /translation="MENPAALLRRLNHYCARALEGAASLCQTRAHAEITPEHWLLKLL
                     EQGEGDLTVLGRRYDWDMDAIWQSLLGWLDNQPRSVRSRPQLAQSLNALLKQAWMVAS
                     LQGEEHIRSVHLLGALTENPHLVRCDGVWPLLTLSHSQLQRLSPLLDAQSDECPERLQ
                     DAEPVLPQGDNVTFIGRPVGADTAGIPSGDLPPVLQSALDKFTRDITASAREGKIDPV
                     SGRDTEIRQMVDILSRRRKNNPILVGDPGVGKTALVEGLALRIAEGNVPESLRPVTLR
                     "
BASE COUNT          197 a          256 c          305 g          198 t
ORIGIN      
        1 ttttttcttc agtatctgtt ggcgggtgct gaagaaagaa ttctcaatcc tgaacacaga
       61 cctgatgcgc ccgggttatg gcgaagggcg gtagcgtgtc tgaaggtctt ctgacagtga
      121 gtgaataaca tggaaaatcc ggcagccctg ttacgtcgtc ttaatcatta ctgtgcccgt
      181 gcactggaag gcgcagcctc cctttgccag acccgggccc atgcggaaat caccccggag
      241 cactggttac tgaaactgct ggagcagggg gaaggagacc ttaccgtgct gggccggcgt
      301 tatgactggg atatggacgc aatatggcag tcgctgctcg gctggctgga taaccagccc
      361 cgtagcgtac gcagccgccc gcagcttgcg cagtccctga atgccctgct gaaacaggcc
      421 tggatggtgg cctcattaca gggggaagaa catatccgca gcgtgcatct gctgggtgcc
      481 ctgacggaaa atccgcacct ggttcgctgt gacggggtgt ggccgctgct gacactgagt
      541 catagccagt tgcagcgtct gtccccgctg ctggatgcgc agtctgatga gtgtccggag
      601 agattacagg atgcagagcc tgtgctgcct cagggagaca atgtgacctt tatcgggcgt
      661 cccgtcggtg cggatacggc gggtataccg tcaggtgacc tgccgcctgt gttacagagt
      721 gcgctggata aattcacccg ggacatcacg gccagcgcga gagaggggaa aattgacccg
      781 gtatcgggac gcgatacgga aatccgtcag atggtggata ttctctcccg ccgccgcaag
      841 aacaacccga ttctggtggg ggaccctggt gtggggaaaa cggctctggt ggaagggctc
      901 gccctgcgta ttgcggaagg taacgtaccg gaatcactca gacccgtcac cctgcg
//
LOCUS       DAZGSP010000325          955 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZGSP010000325 DAZGSP010000000
VERSION     DAZGSP010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.329"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            229..819
                     /locus_tag="ACHK74_005388"
     CDS             229..819
                     /locus_tag="ACHK74_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0224010.1"
                     /translation="MSRIFAYCRISTLDQTIENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMHVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT          303 a          203 c          198 g          251 t
ORIGIN      
        1 aataacactc aacactattc ttcttccttg agtccgcccg gaactcgaaa aacaaaccga
       61 gttaaagcca tttttcacaa aatcgatttt gggtctcacc aaaattacgg ggttgcatac
      121 gcattcgttt attttcgaac gtgtacatac aaatatgcac aaaaataatc aaaattattt
      181 tctgagatgc attatgatat gaacaccaat ttcgtataga gtctcactat gtctcgaatt
      241 tttgcttact gtcggatatc aacgctggat cagaccatcg aaaatcaacg ccgggaaatc
      301 gaaagtgcag gttttaaaat caaacctcag caaataatcg aagaacacat tagcggctca
      361 gcagcaacca gtgagcgtcc tggttttaac cggttgcttg ctcgcctgaa atgtggtgat
      421 caattgattg tgacaaaact ggatcgcctt ggttgtaatg caatggatat caggaaaaca
      481 gtggaacaac tgaccgaaac aggtatcaga gtgcattgct tagcattggg gggcattgac
      541 ctgaccagtc caacaggaaa aatgatgatg cacgtaattt cagcagtcgc tgaatttgaa
      601 cgagaccttt tacttgaacg cactcattcc gggatagtaa gagcccgcgg cgcagggaaa
      661 cgttttggtc gaccacctgt gttaaatgaa gaacagaaac aggtggtatt cgaacgaatt
      721 aagtcaggtg taagtataag tgccattgcc cgggaattca aaacctcgcg gcaaaccatt
      781 ttaagagcca aagcaaaact tcagacacct gacatataaa aaataatctc ggtgtgagat
      841 gctttacgtc ttccaagccc ccttccttgc cgtaaatgga aagatacatc taattataga
      901 atttatatgt tttaccctac ggcagtgctg gccattcaat atcctgtgca gttga
//
LOCUS       DAZGSP010000326          940 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZGSP010000326 DAZGSP010000000
VERSION     DAZGSP010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.330"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..364)
                     /locus_tag="ACHK74_005389"
     CDS             complement(<1..364)
                     /locus_tag="ACHK74_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFP0224011.1"
                     /translation="MVFMENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVII
                     GNDVSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTFRELTAQEPEDRTL
                     TLNQQIEVLGLKFAVKEKN"
     gene            613..>940
                     /locus_tag="ACHK74_005390"
     CDS             613..>940
                     /locus_tag="ACHK74_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFP0224012.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIRINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEID
                     ILRLCVS"
BASE COUNT          309 a          183 c          134 g          314 t
ORIGIN      
        1 catttttttc ttttacagca aatttgagac ctaaaacttc tatttgctga ttcaatgtta
       61 acgttctatc ttcaggttct tgtgctgtta attctctgaa tgttactacc agatcagggt
      121 cggtagtaat tattatctca aaactacctg taagctcgcc ataaggaacg actattgtgt
      181 tttctggtaa ctcagaaaaa gcatcacttc ttcctaacga aacatcatta ccaatgataa
      241 caagtagacg cccattaagt atttcatact cgcatccatt caatggtccg ctcaacaaac
      301 gaatggcata agattccaat aagtcttgtg ataagaattt atcattattt tccatgaata
      361 ccacccgcta attccataag ctctagttta gagtagtgta acaagacgga aatttcatga
      421 ccaaattatg atgacacaga ataaaaaaat tataattccg agaactattt taaatgcctc
      481 tatgcataac tttatttaca atttaaggta taaaccctat tccctcaatg ttaagcaaat
      541 ataacaatta ctcttatttt aaattttaca cgtaatattt accctaaatg attttagagt
      601 acaggtgggc atatgcaagt cttttccagt gatgtctact tcactgtagg cactaatgca
      661 ttattagcat cccaaaaaga atattacagc gatttagttg cgttggttga tcttggacat
      721 tcgttcgttg ttattgatga gcatcaacat cgaaatctca aaccaaatac agaacctgta
      781 aacatccttt tgagcaacaa tttcatcagg atcaataaga atattacatt atctgattta
      841 acgcatttcc tggtttcaaa tcttcataca caaaatgtgt attccacaca agaaccactt
      901 acccatgatg agatagatat cctgcggtta tgtgtttctt
//
LOCUS       DAZGSP010000327          939 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZGSP010000327 DAZGSP010000000
VERSION     DAZGSP010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.331"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..828
                     /locus_tag="ACHK74_005391"
     CDS             <1..828
                     /locus_tag="ACHK74_005391"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP0224013.1"
                     /translation="ESSNAIDNADVYHYHRPHLESRLKENSVVTIHHDLYDTDPWLDY
                     DKFHPRYMEAKKILCLNSSQQNYLNNKGLMNTEIIPHGYNSDIFCSVGAQKKIKNKVC
                     IGFISKRYGRKVKGEAYLYEIFKRLDSKRYRFIFVGVDRTITAYKASRFGFEVKCYER
                     LPYYCYGDLYKQLNFLLVTSLYEGGPANIPEAIASGTPIISTPVGMVNDYVRDGINGL
                     ILTGDIDNDIDSINNFSQKSKYQKLSSNALEMSGNIMSWQDVMQKTSLVYKELAGYA"
BASE COUNT          349 a          135 c          165 g          290 t
ORIGIN      
        1 gaaagtagca acgctattga taatgctgat gtctatcact atcatcggcc tcatttagaa
       61 agcagactta aggaaaactc agtagtaaca atacatcatg acttgtatga tacagaccca
      121 tggttggatt atgataagtt tcatccccgt tatatggaag caaaaaaaat actgtgcctt
      181 aactcctctc agcaaaatta tttaaacaat aaaggcttaa tgaatactga aattatacca
      241 catggttaca attctgacat cttttgctca gtaggagcgc aaaaaaaaat aaaaaataaa
      301 gtttgcattg gttttatatc aaagagatat ggacgaaaag tgaaaggaga agcttatctt
      361 tatgagattt ttaaaagatt agattctaaa agatacagat tcatatttgt aggagtcgat
      421 agaacaataa cagcatataa agcatcaaga tttggttttg aagtaaaatg ttatgaaagg
      481 cttccctatt attgttacgg tgatttatat aaacaactta attttctttt agtaacgagt
      541 ttgtacgaag gtggaccagc aaatatacct gaagcaattg catccggtac gccaataata
      601 tcaacccctg tgggtatggt aaatgattat gtcagggatg gtattaatgg tcttatatta
      661 actggagata ttgataatga tatcgattct ataaacaatt tctctcaaaa aagtaaatat
      721 caaaaattat caagtaatgc tcttgaaatg tctgggaaca taatgtcttg gcaggacgtt
      781 atgcaaaaaa catcattggt atacaaagag ttagcagggt acgcttaaat gaaaaatata
      841 agtatattat ttgcggacag tatacatttt catgtgcgta attttaaaag tctattcgat
      901 ttaatcgaca ggcataaaat tcaccatacg ttcatacat
//
LOCUS       DAZGSP010000328          931 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZGSP010000328 DAZGSP010000000
VERSION     DAZGSP010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.332"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..337
                     /locus_tag="ACHK74_005392"
     CDS             <1..337
                     /locus_tag="ACHK74_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224014.1"
                     /translation="FNSNNMAIYWNSRASLFCSTELNSKSQSPALGLGHEFAHAQYYL
                     LDKENFMALLSRTDKKYENKEEARVITIIESRAAKTLGECTRGAHRGLPFYRVDGPLQ
                     TMKITGTPE"
     gene            349..798
                     /locus_tag="ACHK74_005393"
     CDS             349..798
                     /locus_tag="ACHK74_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFP0224015.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEISDLYAGKIIDLLQMKYFRKEKYNNIRWGVSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT          314 a          113 c          232 g          272 t
ORIGIN      
        1 atttaattcg aataatatgg cgatatattg gaatagtaga gcatctttat tttgttcaac
       61 tgaattaaac tcgaaatcac aatcgcctgc attaggtttg ggacatgagt ttgctcatgc
      121 gcagtattac cttcttgata aggaaaattt catggctctt ttaagccgaa cagataagaa
      181 atatgagaat aaagaagagg caagggttat tacaataatt gaatcgcgtg cggcgaaaac
      241 tttaggtgag tgtaccagag gagctcatag aggactgcct ttttatcggg tggatggacc
      301 gttgcagacg atgaaaataa cgggtacgcc agaatagtga ggttttttat gatgaaatat
      361 ttgatggttt tattgtctct attttctggt tcggtactgg ggatggggcg agtgaatgaa
      421 ttgtgtggaa tagatagcgt gaaaacgata gagataatta atcttccatc gtatgttacc
      481 acgttagttc cgcttagcaa ggaaggtctt aatgaaatat acagatataa agtggtggtg
      541 aatgaaatta gcgatcttta tgctggtaag ataattgatt tattgcaaat gaagtatttc
      601 agaaaagaaa aatataacaa tataagatgg ggggttagta tcatttcgaa aggtaataat
      661 aaatgtgaaa tttattttga tgcttttggt gaatgtggaa gtgtgaatgg cattaacgtg
      721 tgttttgaaa aaaatgaaat gataggttgg attaaaaaag aaatcccttt gctgtcgcaa
      781 aaaataggtg ggctttaaat ggtagggcaa tattcaaaga cagtataggt aactatttgt
      841 atggccatgt aaggtaaaag aaaaggaata aaccatgagc ggaaaaccgg cggcgcggca
      901 gggcgacatg acccgcaggg aaagatcatc a
//
LOCUS       DAZGSP010000329          927 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZGSP010000329 DAZGSP010000000
VERSION     DAZGSP010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.333"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            72..338
                     /locus_tag="ACHK74_005394"
     CDS             72..338
                     /locus_tag="ACHK74_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFP0224016.1"
                     /translation="MVKGVVLFGDKTTHGGQVISASSNITVNGKKVALVGDMVSCPVS
                     GHGVNPILEGMPQRTCNGRSVVADGCKCQCGCQVISSAINSTIG"
     gene            425..>927
                     /locus_tag="ACHK74_005395"
     CDS             425..>927
                     /locus_tag="ACHK74_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224017.1"
                     /translation="MIIVGILLFIIHASGHVKTLNMLSIWWFSLTPPGIWFLLFLLRC
                     WQWNNQIDKYLFLKKENEYAQMQWEVWAERYLVISASSVMLPGGVTAGAILKSLADTL
                     PSGYLLTKRLKNINTPVTSALASLQLSICQLPAALPVNVTLITDQPDSEIRSAFVSAW
                     EALFPQRV"
BASE COUNT          237 a          161 c          223 g          306 t
ORIGIN      
        1 agtttgtctt aaatagagat gtgcggaagc tttttgtaat aaatttgttc gatgaaaata
       61 taggagggtg tatggttaag ggggttgtat tgtttggtga taaaacaaca catggcggac
      121 aggtaatttc tgcttcatca aatattactg ttaatggtaa gaaggttgcc cttgtagggg
      181 acatggttag ctgtcctgta tccggacatg gcgttaatcc tatactggaa ggaatgccac
      241 agaggacctg caatggacga tctgttgttg ctgatggctg taagtgtcag tgcggttgtc
      301 aggttatttc cagtgctatc aatagcacga taggttaatt atgggctgga aaagaaatcg
      361 gatatctctt ttaatatcac catctgctcc ttccctgata tggtggtcac tgagcggtat
      421 attagtgatt attgttggta tcctattgtt tattattcat gcttctgggc atgtgaaaac
      481 gttgaatatg ttaagtatct ggtggttttc attaactcca cctggaatct ggtttttgtt
      541 atttctttta cgttgttggc agtggaacaa tcagattgat aaatatcttt ttctgaaaaa
      601 agaaaatgaa tatgctcaga tgcagtggga ggtctgggca gagaggtatc tggttatctc
      661 cgcaagcagt gttatgcttc ctggtggtgt gacagccgga gcaattctga aatcactggc
      721 agatacatta ccttccgggt acttgctgac aaaacgctta aagaatatta atacacctgt
      781 aacttctgcc ctggcaagct tgcaactatc tatttgtcag ttacctgccg ctctccctgt
      841 aaatgtgacg ctgataaccg atcagcctga ttctgaaatc agatctgctt ttgtttctgc
      901 atgggaagcg ttatttcccc aacgcgt
//
LOCUS       DAZGSP010000330          919 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZGSP010000330 DAZGSP010000000
VERSION     DAZGSP010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.334"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            167..>919
                     /locus_tag="ACHK74_005396"
     CDS             167..>919
                     /locus_tag="ACHK74_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFP0224018.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGM"
BASE COUNT          251 a          169 c          232 g          267 t
ORIGIN      
        1 atttttaatc tatcaattcg aaagcattag caaattagcc aaattgacac cttcccacct
       61 ccaactaaca ctcaatacag acctctgaca atttaataag tttcattttt aatgtctgac
      121 gactcatctc tttcgtggct gtattgtttg tatatggagc gtttatatga atagaatcta
      181 tcgcgtgata tggaattgca ctctacaggt atttcaggcc tgctcggaat taactcgcag
      241 ggcaggtaaa acatcgacgg ttaatttgcg taaatcctct ggactgacaa cgaaattcag
      301 tagattgacg ctgggtgttt tgctggcact aagcggttca gcgtctggtg caagtctgga
      361 agttgataat gatcagatta ccaatattga tactgatgtt gcttatgatg cctacctggt
      421 tggctggtat ggcactggag tgcttaatat tttggctggc ggtaatgcct ccttaaccac
      481 tattactacc agcgtcattg gcgctaatga ggactcagag ggcaccgtta atgttttggg
      541 tggcacctgg cgattgtatg atagcggaaa taatgcaagg cctttaaatg tgggtcaatc
      601 cggaacgggg acgctgaata ttaaacagaa gggtcacgtc gatggaggct atttaagatt
      661 aggttcttcg acaggaggcg tcgggacggt caatgttgag ggagaggact ctgttctgac
      721 gaccgaatta ttcgaaatag ggagttacgg tacgggctca ttaaatatta cggataaggg
      781 ttacgtcacg agttcaatcg tcgccatttt aggctatcag gcgggcagta atggccaggt
      841 tgtcgttgaa aagggtggcg agtggctaat aaaaaataac gattcctcaa ttgaatttca
      901 aattggtaat caagggatg
//
LOCUS       DAZGSP010000331          918 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZGSP010000331 DAZGSP010000000
VERSION     DAZGSP010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.335"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(45..371)
                     /gene="ybfB"
                     /locus_tag="ACHK74_005397"
     CDS             complement(45..371)
                     /gene="ybfB"
                     /locus_tag="ACHK74_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HFP0224019.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
     gene            complement(371..>918)
                     /locus_tag="ACHK74_005398"
     CDS             complement(371..>918)
                     /locus_tag="ACHK74_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP0224020.1"
                     /translation="YITQDPIGLKGGWNFYQYPLNPISNIDPLGLETLKCIKPLHSMG
                     GTGERSGPDIWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTKEA
                     AGRCDLVETDNSCVENCLKGKFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDSLETC
                     KMKCSGNNIGRFIRFVFTGVM"
BASE COUNT          298 a          207 c          133 g          280 t
ORIGIN      
        1 tcaatgccca ggttacatat aataataaga gcaattaact taatttattt gtttataatg
       61 cgcttactta atacaaacca ttgaatatac cctcctgaca gaaaataaag ccatattatt
      121 atcgccataa tatacttact atcaatcctt acgccaataa tgtctgatac agtccctata
      181 aatattagag caataaagaa tactatgcct gttggaaaac atattaccat cattccataa
      241 gacatgagac taattaactc gctcacatca cgagtggagt ctaatagtga aagtctatgc
      301 atggaaaaga atagtattaa aagcgataaa gccagccata tagctctgaa taaaaaaata
      361 atatatttca ttacatcact ccggtgaata caaatctaat aaaacgtcca atgttatttc
      421 cggagcactt catcttacat gtttctaaag aatcattaga ccagtcttgg cagtttttaa
      481 atagccctaa atttataggt gtgaatatat caggcaatac agaataacgc ggccttacct
      541 ctttaaactt cccttttaaa cagttctcca cacaactatt atcggtttca acgaggtcac
      601 aacggccagc agcttctttt gtatcattac ttgctttacc tggcccccat agaccatctc
      661 cttttgattc tccccgttgg tcttggccac cacaagtata gtccccttta ccatctggga
      721 cacaaagata ttgatgatag aacggattcc cccatatatc tggaccgctt ctttcaccag
      781 ttccgcccat tgaatgcagt ggcttaatgc attttagtgt ttctaatcct aatggatcta
      841 tatttgagat cggattcaac ggatactgat aaaaattcca tccccccttc agcccaatcg
      901 gatcctgagt gatatacc
//
LOCUS       DAZGSP010000332          900 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZGSP010000332 DAZGSP010000000
VERSION     DAZGSP010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.336"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>900
                     /locus_tag="ACHK74_005399"
     CDS             <1..>900
                     /locus_tag="ACHK74_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP0224021.1"
                     /translation="VSVAQKSFGKEMAMIMFFAMVINIMIARFTPWKFIFLTGHHTLF
                     MSMMVAVILATAGMTGITLIAVGSLVVGVAMVFFPAIAHPYMKKVTGSDDVAIGHFST
                     LSYVLAGFIGSKFGNKEHSTEDMNVPKSLLFLRDTPVAISFTMSIIFLVTCLFAGADA
                     VKELSGGKNWFMFSIMQSITFAAGVYIILQGVRMVIAEIVPAFKGISDKLVPNARPAL
                     DCPVVFPYAPNAVLVGFLSSFAAGLIGMFTLYLLNMIVIIPGVVPHFFVGAAAGVFGN
                     ATGGRRGAILGAFAQGLLITFLPV"
BASE COUNT          179 a          202 c          241 g          278 t
ORIGIN      
        1 gtttccgtag cgcagaaaag cttcgggaaa gaaatggcga tgattatgtt ctttgcgatg
       61 gttatcaaca ttatgattgc ccgtttcaca ccgtggaaat ttatcttcct gaccggtcat
      121 cacacgttgt ttatgtcgat gatggtagcg gtaattctgg caacagcagg catgaccggc
      181 attacgctta tagccgttgg ctctttggtt gttggcgtgg caatggtctt tttcccggct
      241 attgcgcatc cgtacatgaa gaaagtgacc ggttctgatg acgttgccat cggccacttc
      301 tcaacattat cttatgtatt ggccggtttc atcggcagca agtttggtaa taaagagcac
      361 tcgactgaag acatgaatgt gccgaaaagt ctgctgttct tgcgcgacac gccggttgct
      421 atctctttta ccatgagcat aattttcctg gtgacgtgcc tgtttgcggg tgcagatgcg
      481 gtgaaagagc ttagcggtgg taaaaactgg ttcatgttct ctatcatgca atccattacc
      541 ttcgcagctg gcgtgtacat cattctgcag ggtgtgcgca tggtgattgc ggagattgtc
      601 ccggcattca aaggtatttc agacaagctg gttccgaatg ccagacctgc tctcgattgc
      661 ccggtcgtct tcccttacgc acctaacgct gtactggttg gtttcctgag tagctttgct
      721 gcaggtttaa tcggtatgtt cacgttgtat ctgctgaaca tgatcgtgat tattcctggt
      781 gtggtgccgc acttcttcgt aggtgcagct gcaggggtat tcggtaatgc aacgggtgga
      841 cgtcgtggtg cgattttggg tgccttcgct cagggcttgc ttattacctt cctgccagta
//
LOCUS       DAZGSP010000333          899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZGSP010000333 DAZGSP010000000
VERSION     DAZGSP010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.337"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..220)
                     /locus_tag="ACHK74_005400"
     CDS             complement(<1..220)
                     /locus_tag="ACHK74_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224022.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKSVNPVKITMGSGLNY
                     IEQESLGGKYLTHDLSIKIADISEEIIQQ"
     gene            414..839
                     /gene="yqeK"
                     /locus_tag="ACHK74_005401"
     CDS             414..839
                     /gene="yqeK"
                     /locus_tag="ACHK74_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFP0224023.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRFVNVNSVNKVNVKSKVKSSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
BASE COUNT          285 a          136 c          162 g          316 t
ORIGIN      
        1 cttgctgaat tatctcttca gaaatatccg ctatctttat tgacaaatca tgtgttagat
       61 attttcctcc aagagattct tgttcaatat aatttaaacc agaccccatt gtgattttaa
      121 ctggattaac ggatttctct tcatttttct tatttttgtt ctcctgatca ggaactaatg
      181 gttgcagtgg taggctatta ttaatatttc gtggcttcat tttacatttc tctcatttga
      241 ttagagcaaa atccgaattc gcagcttctg ataatgtcaa atatcacctt atggagacgt
      301 tacgtagaat gactatattt gttatcggca cattaggggt attaatttta gtattgatgg
      361 aaggtgatta ttgaacagta acatcgagtc acggtaaaaa tgtgaggtat ataatggaca
      421 ttgagttttc gcagattcat gaaatggtct atatgcatga tattgtaaat tcggactcga
      481 aaaagaaacc aaggattccg ttaaaaaaat tccttaatgc tgagaaggtt ctcacgcaaa
      541 caacatcatg ggcattaaat tcaagatttg tgaatgttaa ttctgtcaat aaagtcaacg
      601 taaagagtaa agttaaaagc agttatatct cacgatctgt caatgatgag tttagtttaa
      661 cagatgatga aatcaattct tttaaagaaa cgttagtcct ttcctctatt gattcactga
      721 gtaaactggt tcttaataat cctctttctg tattgtttac atcgactgta cgaaggaata
      781 ataatcgagc taagatgaat gtcgagttcg actcatggat ttgcaccaga tgttgttgat
      841 acatgatatt ttacattgca aggagatgtc taaaatgaaa gatgttgatc aaaactttg
//
LOCUS       DAZGSP010000334          891 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZGSP010000334 DAZGSP010000000
VERSION     DAZGSP010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.338"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            48..833
                     /locus_tag="ACHK74_005402"
     CDS             48..833
                     /locus_tag="ACHK74_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP0224024.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          294 a          182 c          191 g          224 t
ORIGIN      
        1 aatgctaaat attctaatca ttatgacagg cgagggagtg tccaattatg aattcaaaaa
       61 agctttgttg catatgtgtg ttattctcgc tgcttgcagg atgtgcctct gaatcttcta
      121 ttgatgaaaa gaagaaaaaa gctcaagtca cacaaagtaa tattaataaa aacactcccc
      181 agcaactgac agacaaagat ttattcggta atgaaaccac tctggccgta tccgaagagg
      241 atattcaagc tgcgcttgat ggagatgagt tccgcgttcc ccttaattct cctgtaatac
      301 ttgttcaatc cggcaaccgc gcaccggaaa ccattatgca ggaggagatg cgaaaatatt
      361 atactgtttc cacattctct ggtatcccgg acaggcaaaa gcctctgact tgtaacaaaa
      421 acaaagataa aaatgagaac gaggatgttg ccagtgctga gaatatgaac tggatgcagg
      481 cactgcgttt tgtggctgca aaaggacatc agaaagcgat tattgtttac caggatacat
      541 tgcagacagg aaaatatgac tctgcgctga aatcaacagt atggtccgac tataaaaatg
      601 aaaaactcac cgacgctatc tccctgcgct acctggtacg tttcacgctg gtggatgtgg
      661 caacaggtga gtgggctacc tggtcgccgg tgaattatga atataaagtg cttccaccat
      721 tgcccgacaa gaatgaagcc agtacgactg atatgacaga gcagcaaatc atgcaactta
      781 agcaaaagac ctataaagcg atggtaaaag atttggttaa ccgctatcaa taataaatta
      841 tatctgccgc caggaatttc tggcggcaat aatacaaaat tctttgcata a
//
LOCUS       DAZGSP010000335          888 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZGSP010000335 DAZGSP010000000
VERSION     DAZGSP010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.339"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..888
                     /locus_tag="ACHK74_005403"
                     /pseudo
     CDS             1..888
                     /locus_tag="ACHK74_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
BASE COUNT          221 a          252 c          275 g          140 t
ORIGIN      
        1 ggtgtatgac cgtagtggca aacaggtgcg cagctttctt acgatgataa ataccggggc
       61 cggatggtgg cgcaccgtca cacgggccga ccggaaatcc gttaccgtta cgacagcgac
      121 gggcgggtga cagaacagct aaacccggca ggcttaagtt acacgtatca gtatgaaaaa
      181 gaccgcatca ccatcaccga cagcctgaac cgccgtgaag tgctgcacac gcagggcgaa
      241 gccgggctga agcgggtggt gaaaaaggaa cacgcggacg gcagcgtcac gcagagtcag
      301 tttgacgcgg tgggcaggct taaggcacag acggatgccg caggccggac aacagaatac
      361 agcccggatg tggtgacggg cctcaccacg ccggatggca gggcatcggc gttttactat
      421 aaccaccaca gccagttaac gtcagccacc gggcctgacg ggctggaatt gcgccgggaa
      481 tatgatgaat ccggccgtct gattcaggaa actgcccctg acggcgatat cacccgctac
      541 cgttatgata atccacacag tgacctcccc tgcgcaacgg acgatgccac cggcagccgg
      601 aaaaccatga cgtggagccg ttacggccag ttgctgagct tcaccgactg ttccggttat
      661 gtaacccgtt atggccatga ccgcttcggg cagatgacgg cggtgcaccg cgaggaaggg
      721 ctgagccagt accgtgcata cgacagccgt ggacagttaa ttgccgtgaa agacacgcag
      781 ggccatgaaa cgcggtatga atataacgcc gccggtgacc tgaccaccgt cattgccccg
      841 gacggcagca gaaacgggac acagtacgat gcgtggggaa aggccatc
//
LOCUS       DAZGSP010000336          866 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZGSP010000336 DAZGSP010000000
VERSION     DAZGSP010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.340"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>866
                     /locus_tag="ACHK74_005404"
     rRNA            <1..>866
                     /locus_tag="ACHK74_005404"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          244 a          187 c          268 g          167 t
ORIGIN      
        1 tgtgtgttag tggaagcgtc tggaaaggcg cgcgatacag ggtgacagcc ccgtacacaa
       61 aaatgcacat gctgtgagct cgatgagtag ggcgggacac gtggtatcct gtctgaatat
      121 ggggggacca tcctccaagg ctaaatactc ctgactgacc gatagtgaac cagtaccgtg
      181 agggaaaggc gaaaagaacc ccggcgaggg gagtgaaaaa gaacctgaaa ccgtgtacgt
      241 acaagcagtg ggagcacaga gcaatctgtg tgactgcgta ccttttgtat aatgggtcag
      301 cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa ccgagtctta
      361 actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca tgggcaggtt
      421 gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa ttagcggatg
      481 acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct ccccgaaagc
      541 tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc ggcaaggggg
      601 tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta tcacgggaga
      661 cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg ccagctaagg
      721 tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc aggatgttgg
      781 cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt cggcctgcgc
      841 ggaagatgta acggggctaa accatg
//
LOCUS       DAZGSP010000337          865 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZGSP010000337 DAZGSP010000000
VERSION     DAZGSP010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.341"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(246..788)
                     /gene="fimA"
                     /locus_tag="ACHK74_005405"
     CDS             complement(246..788)
                     /gene="fimA"
                     /locus_tag="ACHK74_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP0224025.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          265 a          177 c          168 g          255 t
ORIGIN      
        1 atcaataact ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa
       61 ataagaaata taaagaaatg attgctttgg tttcttacaa cacatcattc ccttttcatt
      121 taataaagcg gcatgataca aagtgaacat gatttcgatt tacattgcca ccataccgct
      181 tcagacaacc ggtacattac tttgttataa gacgtgatat ataattcaaa acaacgtggt
      241 tttgattatt cgtatctcat aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca
      301 cttgtaccgg tgcctttata acgtgcagaa aaatgaagaa cgttggttcc ggggatcaag
      361 ttctggttgg ttgagaagct attcccatca ggtttgagaa ttgcggatgt attatcaagt
      421 atttcaatac cgacacccgt tgctgttgtt gtattggtac tggatgcaat ggccaataaa
      481 ttatcattga ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc ggcaacaaca
      541 ggatcgcagt cgttcaactg aatggtgaat ggaattaatg cagaggtatt accaacagca
      601 ttgaaaatat cggtacgata ttgaccgagt gtgacaacct ggtctgccga gtcagtattc
      661 actgaacagg cagcattcac cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg
      721 gcagcataac tacccgtagc ggcgaatagt gcggcagcca gcgcagacga aataaatctt
      781 aatttcatat atattccttc aatctcattt atcgactcca catccgtata taaccgatta
      841 ctttatttaa gacactgata gtagt
//
LOCUS       DAZGSP010000338          860 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZGSP010000338 DAZGSP010000000
VERSION     DAZGSP010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.342"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>860
                     /locus_tag="ACHK74_005406"
     rRNA            <1..>860
                     /locus_tag="ACHK74_005406"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          236 a          196 c          259 g          169 t
ORIGIN      
        1 cgaatgctga cataagtaac gataaagcgg gtgaaaagcc cgctcgccgg aagaccaagg
       61 gttcctgtcc aacgttaatc ggggcagggt gagtcgaccc ctaaggcgag gccgaaaggc
      121 gtagtcgatg ggaaacaggt taatattcct gtacttggtg ttactgcgaa ggggggacgg
      181 agaaggctat gttggccggg cgacggttgt cccggtttaa gcgtgtaggc tggttttcca
      241 ggcaaatccg gaaaatcaag gctgaggcgt gatgacgagg cactacggtg ctgaagcaac
      301 aaatgccctg cttccaggaa aagcctctaa gcatcaggta acatcaaatc gtaccccaaa
      361 ccgacacagg tggtcaggta gagaatacca aggcgcttga gagaactcgg gtgaaggaac
      421 taggcaaaat ggtgccgtaa cttcgggaga aggcacgctg atatgtaggt gaagtccctc
      481 gcggatggag ctgaaatcag tcgaagatac cagctggctg caactgttta ttaaaaacac
      541 agcactgtgc aaacacgaaa gtggacgtat acggtgtgac gcctgcccgg tgccggaagg
      601 ttaattgatg gggtcagcgc aagcgaagct cttgatcgaa gccccggtaa acggcggccg
      661 taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga cctgcacgaa
      721 tggcgtaatg atggccaggc tgtctccacc cgagactcag tgaaattgaa ctcgctgtga
      781 agatgcagtg tacccgcggc aagacggaaa gaccccgtga acctttacta tagcttgaca
      841 ctgaacattg agccttgatg
//
LOCUS       DAZGSP010000339          852 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZGSP010000339 DAZGSP010000000
VERSION     DAZGSP010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.343"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            29..658
                     /locus_tag="ACHK74_005407"
     CDS             29..658
                     /locus_tag="ACHK74_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFP0224026.1"
                     /translation="MSIEQIINEINVYLNSLKKEDERIDAINKIKIELHRSSPFYTEP
                     VDCVIWIKNNLIEANDYNPNVMAPSEKRLLTHSLLVDGFTQPVVTLAQKGKYTVIDGF
                     HRQMLGKSTTKLEKKLKGYLPVTCIKHDNNFKAYSIASTIRHNRARGKHQIDALSDIV
                     RDLSRMGWDDLRIGSELGMDTDEVLRLKQISGLTELFEEENFSPAWTIK"
BASE COUNT          308 a          153 c          168 g          223 t
ORIGIN      
        1 atatacaaat taaaagaaat agaaacacat gagcattgaa caaatcataa atgaaataaa
       61 tgtatacctt aattctctta agaaagaaga tgaacgcatt gatgcaatta ataaaataaa
      121 gattgagtta cataggagta gtccttttta cacagaacct gttgattgtg ttatatggat
      181 aaaaaacaat ctaattgaag caaacgacta taatcctaac gtcatggctc cctccgaaaa
      241 aaggttatta acacactccc tgttggttga tggattcact caacctgttg tcaccttagc
      301 tcaaaagggg aaatacaccg ttattgacgg atttcacaga caaatgcttg ggaaaagcac
      361 aacaaaacta gagaaaaaac taaaaggata tttacctgtc acatgcatta agcatgataa
      421 taactttaaa gcttactcta tcgcctcgac catcaggcat aacagggcca gaggaaagca
      481 ccagatagac gcgctctccg atatcgtccg agatctttcc cgaatgggat gggatgatct
      541 ccgaataggt agtgaactgg gcatggatac agatgaggta ctgagattaa aacaaataag
      601 tgggttaaca gaactatttg aggaggaaaa ctttagtccg gcatggacaa taaagtaatc
      661 atttaggggg tgactttatc cagtaattac cgactgatat atgatcaccc catgtaatgg
      721 acagctattg acagatcaaa aacatggggg atattaattc aactctgaat actcgctaat
      781 gtagtggtca agtaatattg gccacggttt tacagtaaaa atggtatctg ttctctgact
      841 cttccggcgt ca
//
LOCUS       DAZGSP010000340          851 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZGSP010000340 DAZGSP010000000
VERSION     DAZGSP010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.344"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>851
                     /locus_tag="ACHK74_005408"
     CDS             <1..>851
                     /locus_tag="ACHK74_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0224027.1"
                     /translation="ALGVEVTKAKPVTRTITSANIDRLRVTFGVQSLVETTSKGDRNP
                     SSVRLLIQLERNGHWVTEKDVTINGKTTSQYLTSVILNNLPERPFNIRMVRETADSTT
                     DQLQNRTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQMVVNYHIRGRIIQVPSN
                     YDPEKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQ
                     YCDQTVPDGFGGTEPRMTFNAYLSQQRKVWDVLGDFCSAMRCMPVWNGQTLTFVQDRP
                     SDVVWPYT"
BASE COUNT          186 a          231 c          274 g          160 t
ORIGIN      
        1 gcactgggcg tggaggtgac gaaggcaaag ccggtgacgc gcaccatcac gtcagcgaac
       61 attgaccgtc tgcgggtcac cttcggggtg cagtcactgg tggagaccac ctcaaagggt
      121 gaccgtaatc cctcttctgt ccgcctgctg attcagcttg agcgtaacgg gcactgggtg
      181 acggagaagg atgtcaccat taacggcaag accacctcgc agtacctgac gtcggtgatt
      241 ctgaataatc tccctgagcg cccctttaac atccggatgg tcagggagac ggcggacagc
      301 accacggacc agctgcagaa cagaacgctg tggtcgtcat acaccgaaat catcgatgtg
      361 aaacagtgct acccgaacac ggccattgtg gggctgcagg tggatgcgga gcagttcggt
      421 ggccagcaga tggtggtgaa ctaccatatc cgcggccgca tcattcaggt gccgtcaaac
      481 tatgacccgg aaaaacgcac ctacagcggt atctgggacg ggagtctgaa accggcatac
      541 agcaataacc cggcctggtg cctgtgggac atgctgaccc acccgcgcta cggcatggga
      601 aaacgcctgg gggccgcgga tgtggacaag tgggcactgt atgccatcgg gcagtactgt
      661 gaccagacgg tcccggatgg tttcggggga acagagccgc ggatgacttt caatgcgtac
      721 ctgtcacagc agcgtaaggt gtgggatgtc ctgggggatt tctgctcggc gatgcgctgt
      781 atgccggtat ggaacggcca gacgctgacg ttcgttcagg accgtccgtc ggatgtggtg
      841 tggccgtaca c
//
LOCUS       DAZGSP010000341          847 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZGSP010000341 DAZGSP010000000
VERSION     DAZGSP010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.345"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>847
                     /locus_tag="ACHK74_005409"
     CDS             <1..>847
                     /locus_tag="ACHK74_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0224028.1"
                     /translation="GVEVTKAKPVTRTITSANIDRLRVTFGVQSLVETTSKGDRNPSS
                     VRLLIQLERNGNWVTEKDITINGKTTSQYLTSVILNNLPERPFNIRMVRETADSTTDQ
                     LQNRTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQMVVNYHIRGRIIQVPSNYD
                     PEKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQYC
                     DQTVPDGFGGTEPRMTFNAYLSQQRKVWDVLGDFCSAMRCMPVWNGQTLTFVQDRPSD
                     VVWPYT"
BASE COUNT          185 a          230 c          271 g          161 t
ORIGIN      
        1 tgggcgtgga ggtgacgaag gcaaagccgg tgacgcgcac catcacgtca gcgaacattg
       61 accgtctgcg ggtcaccttc ggggtgcagt cactggtgga gaccacctca aagggtgacc
      121 gtaacccctc ttctgtccgc ctgctgattc agcttgagcg taacggtaac tgggtgacgg
      181 agaaggatat caccattaac ggcaagacta cctcgcagta cctgacgtcg gtgattctga
      241 ataatctccc tgagcgcccc tttaacatcc ggatggtcag ggagacggcg gacagcacca
      301 cggaccagct gcagaacaga acgctgtggt cgtcatacac cgaaatcatc gatgtgaaac
      361 agtgctaccc gaacacggcc attgtggggc tgcaggtgga tgcggagcag ttcggtggcc
      421 agcagatggt ggtgaactac catatccgcg gtcgcatcat tcaggtgccg tcaaactatg
      481 acccggaaaa acgcacctac agcggtatct gggacggcag cctgaaaccg gcatacagca
      541 ataacccggc ctggtgcctg tgggacatgc tgacccaccc gcgctacggc atgggaaaac
      601 gcctgggggc ggcggatgtg gacaagtggg ctctgtatgc catcgggcag tactgcgacc
      661 agacggtccc ggatggtttc gggggcacag agccgcggat gacctttaat gcgtacctgt
      721 cacagcagcg taaggtgtgg gatgtcctgg gggatttctg ctcggcgatg cgctgtatgc
      781 cggtatggaa cggccagacg ctgacgttcg ttcaggaccg tccgtcggat gtggtgtggc
      841 cgtacac
//
LOCUS       DAZGSP010000342          843 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZGSP010000342 DAZGSP010000000
VERSION     DAZGSP010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.346"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>843
                     /locus_tag="ACHK74_005410"
     CDS             <1..>843
                     /locus_tag="ACHK74_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224029.1"
                     /translation="HTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTE
                     YGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRREYDEPGRLVSETSR
                     SGETVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQ
                     MTAVHREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQY
                     DAWGKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSDFSYDALDRLVQQGGFDGRTQ
                     RYHYD"
BASE COUNT          198 a          230 c          296 g          119 t
ORIGIN      
        1 catacagaag gcggggccgg gctgaagcgg gtggtgaaaa aagaactggc ggacggcagc
       61 gtcacgcaca gcgggtatga cgcggcagga aggctcacgg cgcagacgga cgcggcggga
      121 cggcggacag agtacggtct gaatgtggtg tccggcgata tcacggacat caccacaccg
      181 gacgggcggg agacgaaatt ttactataac gacgggaacc agctgacggc ggtggtgtcc
      241 ccggacgggc tggagagccg ccgggaatat gatgaaccgg gcaggctggt atcggagaca
      301 tcgcgcagcg gggagacagt acgctaccgc tacgatgacg cgcacagtga gttaccggcg
      361 acgacaacgg atgcgacggg cagcacccgg cagatgacgt ggagccgcta cgggcagttg
      421 ctggcgttca ctgactgctc gggctaccag acccgctatg aatacgaccg cttcggccag
      481 atgacggcgg ttcaccgcga ggaaggtatc agcctttacc gccactatga caaccgtggc
      541 cggttaacct cggtgaaaga cgcacagggc cgtgaaacgc agtatgagta caacgccgca
      601 ggcgacctga ctgccgttat caccccggac ggcaaccgga gcgagacaca gtacgatgcg
      661 tggggaaagg cggtcagcac cacgcagggc gggctgacgc gcagtatgga gtacgatgct
      721 gccggacgtg tcatcagtct gactaacgag aacggcagcc acagcgactt cagctacgat
      781 gcgctggacc ggttggtaca gcagggcggc tttgacgggc ggacgcaacg ttatcattat
      841 gac
//
LOCUS       DAZGSP010000343          834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZGSP010000343 DAZGSP010000000
VERSION     DAZGSP010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.347"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            35..>834
                     /locus_tag="ACHK74_005411"
     CDS             35..>834
                     /locus_tag="ACHK74_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224030.1"
                     /translation="MSKKITKTLLSSSLVVGLLSVSSFTRANEYSYSALDVLGKHGST
                     YTIVYNSDSDTAYVKWKKVPSGVEGIKDGDIVQGLSKINKDGTVEVTTKDGTSTYRVY
                     DREFHNLMNSLVKQDKENTLGKKEGTQTDLVSDADIRNLFGRIAAISEMETNVGLDEY
                     GRVATSAESIKERVSLQTYLATEAANSAIGLDKNGKLTLNETDIVTPLRINDGVVTIA
                     KDVEEQKEGLAIQKKGLSELSSRTDIIDAATAANTNKIDSRTTVGVNA"
BASE COUNT          274 a          136 c          201 g          223 t
ORIGIN      
        1 atttgtatac agataattta acaggatatc cataatgagt aaaaaaatca caaagacact
       61 tctgtcatct tctctggttg taggtctgtt gagtgttagt tcttttacac gtgcaaatga
      121 atatagctac agcgccttgg atgtgttagg taaacacgga agtacttaca ccattgttta
      181 taatagtgat tctgataccg cttatgttaa atggaaaaaa gtacctagcg gtgttgaggg
      241 gattaaagat ggagatattg tacaaggatt atcaaaaatt aataaagatg ggactgtaga
      301 agttactacc aaggatggca ctagtacata tcgtgtgtat gaccgggagt ttcataacct
      361 aatgaattca ctggtcaagc aggataaaga aaacactctt ggaaagaaag agggtactca
      421 aacagacctt gtatctgatg ctgatattcg gaatcttttt ggcagaattg ctgcgatctc
      481 tgaaatggaa actaatgttg gtctggatga atatgggcga gtggcaacta gtgcagagag
      541 tattaaagaa cgcgtgtctt tgcagactta tctggctaca gaggctgcaa atagcgcaat
      601 tggcctagat aaaaacggta agctaactct gaatgagaca gatattgtaa cgccgttacg
      661 tatcaatgac ggcgttgtca ctatagcgaa agatgtggaa gaacagaaag aagggctggc
      721 gatccagaag aaaggactgt ctgagctttc atcccgcaca gatatcattg atgcagcaac
      781 ggctgcgaat acgaataaaa ttgattcccg aaccaccgtt ggtgtgaatg cgga
//
LOCUS       DAZGSP010000344          807 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZGSP010000344 DAZGSP010000000
VERSION     DAZGSP010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.348"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..81)
                     /locus_tag="ACHK74_005412"
     CDS             complement(<1..81)
                     /locus_tag="ACHK74_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224031.1"
                     /translation="MKIYRPLWEDGAFLMPQQFQQQAAWDV"
     gene            complement(97..>807)
                     /gene="tssC"
                     /locus_tag="ACHK74_005413"
     CDS             complement(97..>807)
                     /gene="tssC"
                     /locus_tag="ACHK74_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP0224032.1"
                     /translation="VRSFNYVEEVKGPDHHKYLWTNASFAFAANMVRSFVTNGWCVQI
                     RGPQAGGAVQDLPIHLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYA
                     CFFSANSTQKPALYDTPDATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRL
                     LELELNNWIRGLVTEMTDPGDELQASHPLRDGKVVVEDIEDNPGFFRVKLYAVPHFQV
                     EGMDVSLSLVSQMPKAKA"
BASE COUNT          201 a          224 c          197 g          185 t
ORIGIN      
        1 aacatcccag gcagcctgct gctgaaactg ctggggcatc agaaaagccc cgtcttccca
       61 cagtggacga taaatcttca tttcccgacg cctgccttac gcttttgcct tcggcatctg
      121 cgaaaccagt gacagactga cgtccatacc ttccacctgg aaatgtggta cagcatagag
      181 tttcacgcgg aaaaatcccg ggttgtcttc gatgtcttcc accaccactt ttccgtcacg
      241 cagcggatgt gaggcctgca gttcatcacc cggatcggtc atctccgtca ccagaccccg
      301 gatccagtta ttcagttcca gttcaagcag acggcgatct ttggtggtac cgatattctc
      361 cctctgaatg atcttcaggt aatgcgcaat gcgggacagc aggaagatat agggaagacg
      421 ggcattaata cggctgttcg ccgttgcatc cggcgtatcg tacagtgccg gtttctgggt
      481 ggagttagcg gagaagaagc aggcataatc ccggttcttg tagtaagaga gcggaataaa
      541 gcccagatta gcgaattcaa actcccgagt ttccggaatc atcacctctg acggaatttt
      601 gacctgatta ccggttccca gatcatacag atggatcggc aaatcctgca ccgcaccgcc
      661 ggcctgagga ccgcggatct gaacacacca gccgttggtg acaaaactgc gcaccatatt
      721 ggccgcaaaa gcaaaagacg catttgtcca gagatactta tgatgatccg gacctttcac
      781 ctcttcaaca tagttaaagc tgcggac
//
LOCUS       DAZGSP010000345          785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZGSP010000345 DAZGSP010000000
VERSION     DAZGSP010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.349"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>785
                     /locus_tag="ACHK74_005414"
     CDS             <1..>785
                     /locus_tag="ACHK74_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224033.1"
                     /translation="GPMQLEVPRDQYAGAVETMRNKIREGKVPGVTDPAEASRLIRRG
                     HLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTASVFWLSTGDRDAALQT
                     AAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPTVRQALQKGTGAGNIS
                     ALNKVMKGTLVTSLVLVAVTTGPDMIKMLRGRISGAQFIRNLAVASSGVAGGAVGSVA
                     GGILFSPLGPFGALTGRVAGGVLGGMIASAVSGKIAGALVEED"
BASE COUNT          161 a          205 c          244 g          175 t
ORIGIN      
        1 atggccccat gcaactggaa gtcccccgtg atcagtatgc cggcgctgtt gaaaccatga
       61 ggaataagat ccgcgaaggt aaagtacccg gtgtaaccga tcccgcagaa gcgtcccggc
      121 tgattcgtcg gggacatctg acttatactc aggcccgtaa tatcacccgg ttcgggacca
      181 tcgaatcggt cacttatgat attgccgagg gttcggttgt cagtctggcg gccggaggga
      241 tcagttttgc cctgacggca tcggtcttct ggctcagcac cggcgatcgc gatgctgccc
      301 tgcagacagc tgctgtccag gcaggaaaaa ccttcacccg cacactggct gtctacgtca
      361 caacccagca actccaccgg ctcagtgttg ttcagggtat gctgaagcat attgattttt
      421 cgacggccag cccgactgtc cggcaggcgc ttcagaaggg gaccggtgca ggaaatatca
      481 gtgccctgaa caaagtgatg aagggtacgc tggtgacatc tctggtactg gtcgctgtca
      541 caaccggccc tgacatgatc aaaatgttgc ggggacggat ctccggtgcg cagttcatca
      601 ggaatcttgc cgtggcatct tccggtgtgg caggtggtgc tgtcgggtca gtggcgggcg
      661 ggatactgtt cagtccactg ggaccatttg gtgcactgac agggcgtgtg gctggcggtg
      721 ttctgggggg aatgattgcc tccgctgtat caggaaaaat tgccggagcg ctggttgaag
      781 aagat
//
LOCUS       DAZGSP010000346          785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZGSP010000346 DAZGSP010000000
VERSION     DAZGSP010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.350"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..596)
                     /locus_tag="ACHK74_005415"
     CDS             complement(<1..596)
                     /locus_tag="ACHK74_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase"
                     /protein_id="HFP0224034.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          238 a          189 c          151 g          207 t
ORIGIN      
        1 caaacctctt ctctttttaa tttttcgttt atgagattat ttctttccca tagtccggca
       61 aaacgagcag cattactggc ggtataacgt acagtatggc gaatatttcg atgcccgaga
      121 taatcctgaa ttaaacgggt atcggcacca cgctccgcca gttcataacc acaagcatgc
      181 cttaacatat gaggatgagt ctgcgttacg gttccagctt caataccggc atcgcgaata
      241 atgcgatagg cctgctggcg agaaagccga ctcccgcggc gagaaataaa tatagcgtcg
      301 gtccggtcag cgcctttcca gttagcacgt tcctgggtcc agcgttccac ggcttcacgc
      361 tcatcaaaac gtaacgggtg aacggtagaa aatccgttct tcagtcggcg aatatttatt
      421 cgaccttcat taaggtcaag atcctgataa tgcagatcaa gcagttcact aatacgcatc
      481 ccatgccgat atgccaacag aataagacaa taatctctgg ctcccgttgc cccgtaacaa
      541 accgcctgca tcatggcctg aacttcttta ccggtaagat aacgacgttt actcacaata
      601 gtagtgctcc taactgagat atatttgaat gaatacctat aggaaacctc aatcggtcaa
      661 aattagccca atagataaaa agataaccac acacgaagtg atgtggctat cagtcaatta
      721 cattaaatat catacaaaaa taaaatatca ctgatgtgac aacatctatt ttcattgatt
      781 tgatt
//
LOCUS       DAZGSP010000347          783 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZGSP010000347 DAZGSP010000000
VERSION     DAZGSP010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.351"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            84..>783
                     /locus_tag="ACHK74_005416"
     CDS             84..>783
                     /locus_tag="ACHK74_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224035.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSIFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPITFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQNLSGFVLTLSRF"
BASE COUNT          304 a          151 c          121 g          207 t
ORIGIN      
        1 tgtgtgtaga agaaaatcca ttcctgttct aattattcaa tttaaaaacc ttagcataaa
       61 acagatgatg aataaggaat gttatgtcaa ttgactttac cccaggtata ataaatacat
      121 atcacggcga tatttataac tgcacaacaa ataccgataa tgttaaaacg ccagacacac
      181 cgaagtggcc ttgtgataat tgggaagaac aacaacccat taattccata ttctctggag
      241 aaggatacaa ccctgaacaa tttgatttag cgcaacaaca actccaacaa attaatgcat
      301 gccacacgaa caccacatat acaaatgcag actactcaaa agttgtggtt caacttgtaa
      361 gtcttattaa taacattgaa acgataagtt cgacaccact tacgcaacag acacaatcaa
      421 tacttaatca gattaataac attagatatg aaaaaaacaa aagtgctgaa tgtcgaatca
      481 ttgttgtcgc taatcctaaa cccgacaagg caatcataac taaaattagc gtagaagagg
      541 gaatacccat aacattcagt gtccaaacca tgttttcaga tactaatttt attgctgaac
      601 aacgagctga cttaccaact aatataaaag atattcaaag cctgtatcaa aaaatgacga
      661 agttatatat tgagcacagt gaaaataaaa acaggatgaa agtctttgcc ggaaccaact
      721 ttattgattt caatatgaca ggacagaacc tgtccgggtt cgttctaacc ttgtcaaggt
      781 ttt
//
LOCUS       DAZGSP010000348          778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZGSP010000348 DAZGSP010000000
VERSION     DAZGSP010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.352"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..377)
                     /gene="gmm"
                     /locus_tag="ACHK74_005417"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(<1..377)
                     /gene="gmm"
                     /locus_tag="ACHK74_005417"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFP0224036.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLILPDEQHDD"
     gene            complement(380..>778)
                     /gene="fcl"
                     /locus_tag="ACHK74_005418"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(380..>778)
                     /gene="fcl"
                     /locus_tag="ACHK74_005418"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFP0224037.1"
                     /translation="EATAQNAPDVVVWGSGTPMREFLHVDDMAAASIHVMELAHEVWL
                     ENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASKPDGTPRKLLDVTRLH
                     QLGWYHEISLEAGLASTYQWFLENQDRFRG"
BASE COUNT          173 a          244 c          196 g          165 t
ORIGIN      
        1 taatcgtcat gctgctcatc cggcagtatc agatcttctt cagcgactct gaagcgaaaa
       61 ccgagcacca catagtgagt ggtgaaatcc gtgccggaga agttatcgtc ataaaagtgc
      121 tgccagacac cgtaaaactg gcctgctgtt atcggcagac gcagccccag ttccgccatc
      181 gtcagccgct cgaatgcggc ttccagcgtt tcgtctttct gcacgcgccc tcccggcaca
      241 aaccagtaac cctgcgccgg gcggttggtt cttttgccaa gcagaaactc gccgcgactg
      301 ttctcgacaa taaagtcgag agagacaagc ggggtggagc gcactaccgt ggcaaagtct
      361 tcctgacgta aaaacatcat tacccccgaa agcggtcttg attctcaagg aaccactggt
      421 aagtgctggc aagccccgct tccagtgaga tttcgtgata ccagccaagc tgatgcaggc
      481 gcgtcacatc cagcagtttg cgcggcgtgc catccggttt gctggcatca aaaaccaccc
      541 ggcctttgta acccaccact ttggcgatgg tttgcgccag ctcgcggata gtgcagtcaa
      601 cgcccgtacc aacgttaatg tgcgacagca tcggctgggt gttctccagc cagacttcat
      661 gcgccagctc catgacatga atgctcgccg ccgccatatc atcgacgtgc aggaattcac
      721 gcatcggtgt accgctgccc cacaccacca catccggtgc attctgtgcc gtcgcctc
//
LOCUS       DAZGSP010000349          760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZGSP010000349 DAZGSP010000000
VERSION     DAZGSP010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.353"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..180
                     /locus_tag="ACHK74_005419"
     CDS             <1..180
                     /locus_tag="ACHK74_005419"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224038.1"
                     /translation="EPIATEGLPLCTDLISPIIGRRSANSQYDRHIDRFNANNQIFIL
                     THIAKSAFIKTYIQE"
     gene            469..678
                     /locus_tag="ACHK74_005420"
     CDS             469..678
                     /locus_tag="ACHK74_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0224039.1"
                     /translation="MSEDIPLPKINQRYRDDHGALVTVTSVEETRVVFMRDGYPHPCM
                     RPMYNFLGKFKPEPREAPPAGNHTA"
     gene            complement(698..>760)
                     /locus_tag="ACHK74_005421"
     CDS             complement(698..>760)
                     /locus_tag="ACHK74_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224040.1"
                     /translation="NTAYSEHPEWFSGLVSGDEN"
BASE COUNT          253 a          168 c          157 g          182 t
ORIGIN      
        1 gagcccatcg ccacagaagg cctccccttg tgcactgatt taatttcacc cattatagga
       61 cgaagatctg caaattctca atatgacaga catatcgatc gttttaatgc caataaccag
      121 atctttattc tgactcatat cgcaaaatca gcatttatca aaacttatat ccaggagtaa
      181 tacaaaaatg cagaaaatta ttccggttag tgattttgat acagatggtt ataccgaatg
      241 attaccagca atgacattca cgaggagtga tgattcggca gcatttaagg gcgtatcaga
      301 aaaagagaag gacatgaaag aggagagcaa taaaagacac ggaaaataaa gaagagagag
      361 aaaatatttc ttaaaaacag aagattaaca aaacacatca ggaggcagag actcctgatt
      421 cagcaatatc acatgaaatt ttagttaact gctaccgttc tgaaatctat gtcagaagat
      481 attccattac cgaaaatcaa tcagcgatac agagatgatc atggtgcgct tgtcaccgtg
      541 accagtgttg aggaaacccg ggtggtgttt atgcgggacg gctatccgca tccctgcatg
      601 cggccgatgt acaactttct gggaaaattc aaacctgaac cccgggaagc cccccctgcc
      661 ggcaatcaca ctgcctgatg gcagcataat ccacttatta attctcatcc ccggagacaa
      721 gcccggaaaa ccactccggg tgttcactgt atgccgtgtt
//
LOCUS       DAZGSP010000350          747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZGSP010000350 DAZGSP010000000
VERSION     DAZGSP010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.354"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..376)
                     /locus_tag="ACHK74_005422"
     CDS             complement(<1..376)
                     /locus_tag="ACHK74_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP0224041.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQN"
     gene            complement(476..709)
                     /locus_tag="ACHK74_005423"
     CDS             complement(476..709)
                     /locus_tag="ACHK74_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFP0224042.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDNPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
BASE COUNT          166 a          193 c          205 g          183 t
ORIGIN      
        1 cgttctggca gacgaacctg aagtaacccg gcagcatctg gtagctggag gtaccgtcat
       61 gagagttgag cagaataatt tcagggacat gttctccgtt tatctctccg gcccgccgca
      121 gacgcagcat gtgttttgtg tatccccggc ggcccgggtc gcgcacacgg gtctggcagg
      181 cgaagaatgg ctgaaagcct tcccgctgca ggctttccag tacggtgatg gtggggatgt
      241 acgtatagtt ctggcttcgg gacgtgtgtt tatcttcccc gaaaatcccc ggcacatagt
      301 gcatcagttc ttcgtgtgtc agcggacggt cacggcgtat ctggttcgca taaccaaaac
      361 gactggctag tcgcataatt tgctccttat cggtggttaa gatttactgg tgtaataaat
      421 gaaaaagcca cgtctcccgg agaagacgcg gcctgatggg tgaaataaat gacgtttatt
      481 gtctgagaag accgtcactg gcgagccgcg tattaagcac ctttccggca tcgggttcag
      541 aattccggca gcgccagcgt cgctgacctt ccgcgttgcg gataacccga cggaaatgca
      601 ttcccgggtt atcctggata aacacattgc ggtaagcggt tatgacagca accgcctgcc
      661 cgtatgagaa agatccttca gccaggacat actctgtgta acccggcata tctggtttct
      721 cctgataaat agcctctgcc atacgtt
//
LOCUS       DAZGSP010000351          745 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZGSP010000351 DAZGSP010000000
VERSION     DAZGSP010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.355"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            151..717
                     /gene="slp"
                     /locus_tag="ACHK74_005424"
     CDS             151..717
                     /gene="slp"
                     /locus_tag="ACHK74_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFP0224043.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          227 a          158 c          164 g          196 t
ORIGIN      
        1 acgccccaaa ataacggaat tgaactaaac agaagggata taaacatcag acaggtttac
       61 gttactatca ggcatatcac ctcagaatca gatgaaaact ataaagaaat atctattatg
      121 gttttaatat ttgttgataa ggatagtaac atgaacatga caaaaggtgc actcatcctc
      181 agcctttcat ttttgcttgc cgcatgtagt tcaattccgc aaaatatcaa aggcaataac
      241 caacctgata ttcaaaaaag ttttgttgct gttcataacc agccggggtt atatgttggt
      301 caacaagcgc gctttggtgg gaaggttatc aacgttatca atggcaaaac ggatacgttg
      361 ttagaaatcg ctgtattacc gttggatagc tatgcgaagc ctgatattga agccaactat
      421 cagggccgac tgctcgccag acaaagcggc ttccttgatc cagtgaacta tcgtaatcac
      481 tttgttacca tcctcggtac cattcagggt gaacaaccag gctttatcaa taaagtcccg
      541 tataacttcc tggaagtgaa tatgcagggc atccaggtgt ggcatttgag agaagtggtt
      601 aataccacct ataacctgtg ggattacggc tatggtgcat tctggccgga accgggctgg
      661 ggtgcgcctt actacaccaa tgcggtgagt caggtaacac ctgagctggt caaataacac
      721 caccgaaaga tgcagatgct ttctc
//
LOCUS       DAZGSP010000352          735 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZGSP010000352 DAZGSP010000000
VERSION     DAZGSP010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.356"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>735)
                     /locus_tag="ACHK74_005425"
     CDS             complement(<1..>735)
                     /locus_tag="ACHK74_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224044.1"
                     /translation="QPDGHTLARLWGALPPDIRLSPHLYLATNSAQGPWWILGWSERV
                     PGAEDVLPAPLPPYRVLTGLADRFGRTLTYRREAAGDLAGEITGVTDGAGREFRLVLT
                     TQAQRAEEARTSSLSSSDSSRPLSASPFPDTLPGTEYGPDRGILLSAVWLMHDPAYPE
                     SLPGAPLARYTYTEAGELLAVYDRSNTQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYD
                     DTGRVVEQLNPAGLSYRYQYEQDRITV"
BASE COUNT          118 a          254 c          239 g          124 t
ORIGIN      
        1 gacggtgatg cggtcctgct catactggta gcggtaactc aggcctgccg ggttcagctg
       61 ctccaccacc cgcccggtat cgtcgtagcg gtagcgcatc tccggcctgc ctgcataacg
      121 gtgcgccacc atccggcccg ggtgctgcgc gtcatacgtg aaagcgcgca cctgcgtatt
      181 gctgcggtca tataccgcca gcagttcacc ggcttccgta tacgtgtacc gcgccagtgg
      241 cgcaccgggc aggctttccg ggtatgccgg gtcgtgcatc agccacaccg ccgaaagaag
      301 gatgcccctg tcggggccgt attcggtacc gggcagtgtg tcggggaacg gtgaggctga
      361 gagagggcgg gaactgtcag aagaagatag cgaagaggtg cgggcctctt ccgcccgctg
      421 tgcctgtgtg gtcagtacca gacggaactc acgcccggca ccgtccgtca cgccggtgat
      481 ttccccggcc aggtcgcctg cggcctcacg ccggtacgtc agcgtccgcc cgaaacggtc
      541 tgccagcccg gtaagcaccc ggtacggcgg cagcggcgct ggcagtacgt cctcagcacc
      601 cggcacccgc tctgaccacc ccagtatcca ccacggcccc tgtgcgctgt tggtcgccag
      661 gtaaagatgc gggcttaacc ggatatccgg cggcagcgcc ccccacagcc gcgccagcgt
      721 gtggccgtcc ggctg
//
LOCUS       DAZGSP010000353          728 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZGSP010000353 DAZGSP010000000
VERSION     DAZGSP010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.357"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>728
                     /locus_tag="ACHK74_005426"
     CDS             <1..>728
                     /locus_tag="ACHK74_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0224045.1"
                     /translation="PGKRQEIKAEDINWLPEFLAQYRQSNGRPMTEAYEDFVAEWQHR
                     HADEPYMLDIMPSYDTIRRAMKKLPEVVKQKGRVTGSEYRQLEGFTRRDWSKMPVNYV
                     WIGDGHGMKLKCAHPVHGRPFAPEVTFVIDGGTRFVVGWSLDLAENVFAVAGAIQHGI
                     RHHGKPFLYYSDNGSGETADILDKEVVGILPRLGINHPTGIAGNPQGRGIIERLNRTL
                     PMRIARKYRTYFGKGADRETLRKT"
BASE COUNT          180 a          190 c          211 g          147 t
ORIGIN      
        1 ccgggtaagc gccaggaaat aaaagccgaa gatattaact ggctgcccga atttctggcg
       61 cagtatcgcc agtcaaacgg ccgaccaatg accgaggctt acgaggattt tgtcgctgaa
      121 tggcagcacc ggcacgctga tgagccttat atgctcgata tcatgccgtc ttatgacacc
      181 attcgccgcg caatgaagaa actgccggaa gtggtgaaac aaaaaggccg ggtgaccggc
      241 agtgaatacc gccagcttga gggattcacg cgccgcgact ggtcaaaaat gccggtgaat
      301 tatgtctgga ttggtgacgg tcacggcatg aagctgaaat gcgcacaccc ggttcacggt
      361 cggccatttg caccggaagt gacctttgtt atcgacggtg gcacgcgctt tgtggtgggc
      421 tggagccttg acctggctga aaatgttttc gccgtagccg gtgccataca gcacggcatt
      481 cgccatcacg gcaaaccgtt tctgtattac tcggataacg gctccgggga aaccgccgac
      541 atcctggata aggaggttgt ggggatactg ccgcgactgg ggattaatca cccgaccggg
      601 attgccggta atccgcaggg acgaggcatt atcgaacggc ttaaccgcac attaccgatg
      661 cgcatagccc gtaaataccg cacctatttc gggaaaggtg cagatcgcga gacgttacgc
      721 aaaaccaa
//
LOCUS       DAZGSP010000354          727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZGSP010000354 DAZGSP010000000
VERSION     DAZGSP010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.358"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>727
                     /locus_tag="ACHK74_005427"
     CDS             <1..>727
                     /locus_tag="ACHK74_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP0224046.1"
                     /translation="QPDGHTLARLWGALPPDIRLSPHLYLATNSAQGPWWILGWSERV
                     PGAEDVLPAPLPPYRELTGLADRFGRTLTYRREAAGDLTGEITGVTDGAGREFRLVLT
                     TQAQRAEEARTSSLSSSDSSRPLSASAFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPE
                     SLPAAPLVRYTYTEAGELLAVYDRSNTQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYD
                     DAGRVVEQLNPAGLSYRYQYEQDR"
BASE COUNT          119 a          233 c          258 g          117 t
ORIGIN      
        1 cagccggacg gccatacgct ggcgcggctg tggggggcgc tgccgccgga tatccggtta
       61 agcccgcatc tttacctggc gaccaacagc gcacaggggc cgtggtggat actcggctgg
      121 tcagagcggg tgccgggtgc tgaggacgtg ctgccagcgc cgctgccgcc gtaccgggag
      181 ctgacggggc tggcggaccg cttcgggcgg acgctgacgt accggcgtga ggccgccggt
      241 gacctgaccg gggaaatcac cggcgtgacg gacggtgccg ggcgggagtt ccgtctggtg
      301 ctgaccacgc aggcgcagcg tgcggaagag gcccgcacct cttcgctatc ttcttctgac
      361 agttcccgcc ctctctcagc ctcagcgttc cccgacacac tgcccggtac cgaatacggc
      421 cccgacaggg gtatccgcct ttcggcggtg tggctgatgc acgacccggc atacccggag
      481 agcctgcccg ctgcgccact ggtgcggtac acatatacgg aagccggtga actgctggcg
      541 gtatatgacc gcagcaatac gcaggtgcgc gctttcacgt atgacgcgca gcacccgggc
      601 cggatggtgg cgcaccgtta tgcaggcagg ccggagatgc gctaccgcta cgacgatgcg
      661 gggcgggtgg tggagcaact taacccggca ggcctgagtt accgctacca gtatgagcag
      721 gatcgca
//
LOCUS       DAZGSP010000355          705 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZGSP010000355 DAZGSP010000000
VERSION     DAZGSP010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.359"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..705
                     /locus_tag="ACHK74_005428"
                     /pseudo
     CDS             1..705
                     /locus_tag="ACHK74_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          230 a          121 c          169 g          185 t
ORIGIN      
        1 gtgcagaagg ctgggaagat atagaggatt ttggggaaac acatcccgat gttttgaagt
       61 gatatggtga ttttgaaaat ggtattcctg ttcacgatat cattgccaga gttgtatcct
      121 gtatcagtcc tgcaaaattt cacgagtgct ttattaactg gatgcgtgac tgccattctt
      181 cagatgataa agacgtcatt gcaattgatg gaaaaacgct ccggcactct tatgacaaga
      241 gtcgtcgcag gggagcgatt catgtcatta gtgcgttctc aacaatgcat agtctggtcc
      301 tcggacagat caagacggat gagaaatcca atgagattac agccattcct gaacttctta
      361 acatgatgga tattaaagga aaaatcatca caactgatgc gatgggctgc cagaaagata
      421 ttgcagaaaa gatacaaaaa cagggaggta attatttatt cgcggtaaaa ggaaaccagg
      481 ggcggctaaa taaagccttt gaggaaaaat ttccgctgaa agaattaaat aatccagagc
      541 atgacagtta cgcaatgagt gaaaagagtc acggtagaga agaaatccgt cttcatattg
      601 tttgcgatgt ccctgatgaa cttattgatt tcacgtttga atggaaaggg ctgaagaaat
      661 tatgcgtggc agtctccttt cggtccataa tagcagaaca aaaga
//
LOCUS       DAZGSP010000356          698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZGSP010000356 DAZGSP010000000
VERSION     DAZGSP010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.360"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            45..>698
                     /locus_tag="ACHK74_005429"
     CDS             45..>698
                     /locus_tag="ACHK74_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0224047.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     "
BASE COUNT          230 a          122 c          154 g          192 t
ORIGIN      
        1 cgatttacca gtaatgtaaa taaattttcg aggagatcat tccagtggga cgtaaatggg
       61 ccaatattgt tgctaaaaaa acggctaaag acggtgcaac gtctaaaatt tatgcaaaat
      121 tcggtgtaga aatctatgct gctgctaaac aaggtgaacc cgatccagaa ttaaacacat
      181 ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt tccaaagcac gttattgata
      241 aagcaattga taaagccaaa ggcggcggag atgaaacgtt cgtgcaggga cgttatgaag
      301 gctttggtcc taatggctca atgattatcg ccgagacatt gacttcaaat gttaaccgta
      361 cgattgctaa cgttcgcaca attttcaata aaaaaggcgg caatatcgga gcggcaggtt
      421 ctgtcagcta tatgtttgac aatacgggtg tgattgtatt taaaggatca gaccctgacc
      481 atatttttga aattttactt gaagctgaag ttgatgttcg tgatgtgact gaagaagaag
      541 gtaacattgt tatttatacc gaacctactg accttcataa aggaatcgcg gctctaaaag
      601 cagctggaat cactgagttc tcaacaacag aattagaaat gattgctcaa tctgaagttg
      661 agctttcccc agaagattta gaaatctttg aagggctt
//
LOCUS       DAZGSP010000357          696 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZGSP010000357 DAZGSP010000000
VERSION     DAZGSP010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.361"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            179..544
                     /locus_tag="ACHK74_005430"
                     /pseudo
     CDS             179..544
                     /locus_tag="ACHK74_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          184 a          192 c          170 g          150 t
ORIGIN      
        1 ggactcctta tccacaaaga acaaacacgg cgcgttcgtc ttcatgaaac accggccgcc
       61 tggtgccaca cacgccggga atgctacagc aaaacgaccg gtcgtcgctg cggcagccct
      121 gacagtctca gcaggctgat tctgagcagt attcagccca tagaatgaga taacccgtat
      181 gaatgatgac agacagacgt ctacggtacc cgatgtatgg aacatttgct ttctggctaa
      241 cggcatgccc gacacatggt tttacaccga tgacgcctcc gtcacaaaag agtgggggcg
      301 ggaatattgt gacaatgtgg aagtgttccc tctgtgctat atgcatgacg gtcccgtttt
      361 ccgggggcgc tatattgatg agctcagcgc cgcacggaac agaatgctcc atatgcacca
      421 catactgccg gaatgaaccc ggtacagaga aaattttcat gacgcagaat gtatccgtga
      481 aattcacgaa gcgcccctgc aggctcccgg cattctgcaa aaatacaaac gcacaaaatc
      541 ataaggctgt tctatgtgga tactgatttt ctggctctca tcccccttta atgtctcctc
      601 cggcgatgcc cggcaaccca cactggccgt tcacagtcag gagttccgta ctgaaaaagc
      661 ctgtcgggat gccttcagcg cgctgaaaaa agaaaa
//
LOCUS       DAZGSP010000358          691 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZGSP010000358 DAZGSP010000000
VERSION     DAZGSP010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.362"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..106
                     /locus_tag="ACHK74_005431"
                     /pseudo
     CDS             1..106
                     /locus_tag="ACHK74_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            204..>691
                     /locus_tag="ACHK74_005432"
     CDS             204..>691
                     /locus_tag="ACHK74_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP0224048.1"
                     /translation="MIVCYVILVNRPFETEIAGKKLFFQKNDVLLTDNISQSFFLRHP
                     GNVTELRIDECIVRKYMESADRIQHSDSAVSVPFIKTTFSHIALLHNVIQNLSLQKTL
                     PQQFSEQMFFACLSIFSTHRGFMFLLFRCINSVALKVRYIICSSPSRAWRVKDISNSL
                     YM"
BASE COUNT          194 a          143 c          143 g          211 t
ORIGIN      
        1 agtcagcgct gaacaactgg aactggtgag cagctccctg aaagtgatca gcacagaacg
       61 ggtaaaaaaa gcctgtaaaa aatgtgacgg taccgttgaa gcgccggtct cgtaagaata
      121 atcctgggca aataagattt ttcctgaaaa agcgtctagt cttttgatat cgttgttttt
      181 atgctaatca gggagtatat gtgatgattg tctgttacgt tattcttgtt aaccgtccgt
      241 ttgaaacaga gattgccggg aaaaagctat tttttcagaa aaatgacgtc ctgttgacag
      301 ataacatcag ccagtccttt ttcctgcgcc atcccggtaa tgttactgag ctccggattg
      361 acgaatgcat tgtcagaaaa tatatggaaa gtgccgacag gatccagcac tctgatagtg
      421 ctgtttctgt tccgttcatt aaaacaacct tctctcacat cgctctgtta cataatgtca
      481 tacagaatct cagtctgcaa aagacattgc cacaacaatt ttctgagcag atgttttttg
      541 cctgcctttc cattttttcc acacacagag gtttcatgtt tctgctattc agatgcataa
      601 actctgttgc gcttaaagtc aggtatatta tctgttccag tccatcacgg gcatggagag
      661 ttaaagatat cagtaattct ctgtacatga g
//
LOCUS       DAZGSP010000359          690 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZGSP010000359 DAZGSP010000000
VERSION     DAZGSP010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.363"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>690
                     /locus_tag="ACHK74_005433"
     CDS             <1..>690
                     /locus_tag="ACHK74_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator receiver domain"
                     /protein_id="HFP0224049.1"
                     /translation="LLQDLKDSDHLNMIVIYTRENLETVWMQISSTLKGALDINSLII
                     DYDNEDVQSYWEDVVLPNLNDNGNKALTRDETIAYIKDSKPCRRIKRLIHDDAVLEDQ
                     KDKNFIAKMIAEYAVSRNAIISSNTSGNVIRGDESGVKWIQCGNIFVSLFHKVQDDHE
                     NDGDRIWQTLNDSLIEWKPSYYQLIKSEIQNAIEAEALSFVNHLANDHYGQAAWLNEI
                     LKSDSPDIRCR"
BASE COUNT          249 a           98 c          137 g          206 t
ORIGIN      
        1 actattacaa gatttgaaag actccgatca tttaaatatg attgtaatat atactagaga
       61 gaatttagaa acggtttgga tgcagatatc atcgactctc aaaggtgctc tggatatcaa
      121 cagcttgatc attgactacg ataatgaaga tgtccaaagt tattgggaag acgttgtatt
      181 accgaactta aatgataatg gtaataaagc tctcacaaga gatgaaacaa tagcctatat
      241 taaagacagt aagccttgta gaagaattaa aagattaata catgatgatg ctgtgttgga
      301 ggatcaaaag gataaaaact tcattgcaaa aatgattgca gaatatgctg tgtctagaaa
      361 tgcaattatt tctagcaaca catctggcaa tgtcattcgg ggtgatgaaa gcggagtaaa
      421 atggattcaa tgtggtaata tctttgtctc cctatttcat aaggttcaag atgatcatga
      481 aaacgatgga gataggattt ggcaaactct caatgattct ctcattgaat ggaaaccatc
      541 ttattatcag ttaataaaat ctgaaattca gaatgcaatc gaagctgagg ctttatcttt
      601 tgtaaatcat ttggctaacg atcattacgg tcaagctgcg tggttaaatg agatattaaa
      661 atcagactcg cctgatatta gatgtagaaa
//
LOCUS       DAZGSP010000360          688 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZGSP010000360 DAZGSP010000000
VERSION     DAZGSP010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.364"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>688
                     /locus_tag="ACHK74_005434"
     CDS             <1..>688
                     /locus_tag="ACHK74_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP0224050.1"
                     /translation="TTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSATGPDGLE
                     MRRKYDEYGRLIQETAPDGDITRYRYDNPHSDLPCATDDATGSRKTMTWSRYGQLLSF
                     TDCSGYVTRYDHDRFGQVTAVHREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAG
                     DLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRY
                     DVLDRLIQETG"
BASE COUNT          170 a          204 c          200 g          114 t
ORIGIN      
        1 ggacaacaga gtacagcccg gatgtggtga cgggcctcat cacgcgcatc accacgccgg
       61 atggcagggc atcggcgttt tactataacc accacagcca gttaacgtca gccaccgggc
      121 cagacgggct ggaaatgcgc cgcaaatatg atgagtacgg acgtctgatt caggaaaccg
      181 cccctgacgg cgatatcacc cgctaccgtt atgataatcc acacagtgac ctcccctgcg
      241 caacggacga tgccaccggc agccggaaaa ccatgacgtg gagccgttac ggtcagttgc
      301 tgagcttcac tgactgttcc ggttatgtaa cccgctatga ccatgaccgt tttggtcagg
      361 tgacggcggt gcaccgcgag gaagggctga gtcagtaccg cgcatacgac agccgtggac
      421 agttaattgc cgtgaaagac acgcagggcc atgaaacgcg gtatgaatac aacgccgccg
      481 gtgacctgac caccgtcatt gccccggacg gcagcagaaa cgggacacag tacgatgcgt
      541 ggggaaaagc catctgtacc acgcagggcg gtctgacgcg cagtatggaa tacgatgctg
      601 ccggacgggt catccgcctg accagtgaaa acggcagcca caccaccttc cgttacgatg
      661 tactcgaccg gctgatacag gaaaccgg
//
LOCUS       DAZGSP010000361          687 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZGSP010000361 DAZGSP010000000
VERSION     DAZGSP010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.365"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..207
                     /locus_tag="ACHK74_005435"
     CDS             <1..207
                     /locus_tag="ACHK74_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP0224051.1"
                     /translation="LTLIPSGVNFDADENDPVKLLIVLAATDSTSHIEAISQLAKLFD
                     NEKDIQAILTAKTTQDILSVIARY"
     gene            223..510
                     /locus_tag="ACHK74_005436"
     CDS             223..510
                     /locus_tag="ACHK74_005436"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP0224052.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLVAQSVAGKIVALENMIDKKAMRERLSVALTELGAL"
     gene            523..>687
                     /locus_tag="ACHK74_005437"
     CDS             523..>687
                     /locus_tag="ACHK74_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFP0224053.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGA"
BASE COUNT          186 a          144 c          178 g          179 t
ORIGIN      
        1 ctgaccctga tcccttcagg tgtgaatttt gatgccgatg aaaacgatcc agtgaaatta
       61 ctgattgtgc tggctgcaac cgacagtacc agccacattg aagcgatttc tcaactcgcc
      121 aagttattcg ataacgaaaa agacatacag gctattttga cggccaaaac gacgcaggac
      181 attttgtctg ttattgcgcg ctattaatcc tgaggaacat ttatgaaaat tacagtggtt
      241 tgcggtaacg gcttaggcac cagcctgatg atggaaatga gcattaaaag cattttgaag
      301 gatttgtccg tcagcgctga tgttgatcat gtcgacctgg gctccgcgaa agggacgccg
      361 agtgatatct atgtcggcac caaagacatt gccgagcagt tagtcgcgca gtctgtcgca
      421 ggcaaaatcg tcgcactgga aaatatgatt gataagaaag ccatgagaga acgtttatcc
      481 gtggcattaa ccgaattagg cgcactgtaa gctgggggcg atatggattt ctttcgtttt
      541 ttgatgagcg atgtactttc cgaacccgca gtgctggtcg gtttaatcgc cctgattggc
      601 ctgattgcac agaaaaaacc ggtaaccgaa tgcattaaag gtaccgtcaa gaccattatg
      661 gggtttgtga ttttaggcgc aggggcg
//
LOCUS       DAZGSP010000362          683 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZGSP010000362 DAZGSP010000000
VERSION     DAZGSP010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.366"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..620)
                     /locus_tag="ACHK74_005438"
     CDS             complement(<1..620)
                     /locus_tag="ACHK74_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFP0224054.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVP"
BASE COUNT          165 a          173 c          166 g          179 t
ORIGIN      
        1 ggcaccacgc cgatggtgtt ttcgtcacag gcttcaatca tgcgtttcgg gtccataaac
       61 aactgaccgg ggcgcatagg gatctcacgc agctccacat cccagtagcg ggcgaattta
      121 tgccagcaga tttgtaccgg accgcacacc aggtttggtt tatccgttgg tttgcctgca
      181 gcttccatac gcttgcgcca acgccatttc atcgccatcc cgccgagcat acaggcctcg
      241 gaagaaccaa tggtgttggt gccaacggcc tgaccatttt tcggcgcagg cgcatgccac
      301 agatcggcaa ccatatttac gcaacgcagg tcgatggctg cggattgcgg atattcttct
      361 ttgtcgatcc agtttttatt gatcgacaaa tccatcaatt tatggacgtt ttcgtcgtcc
      421 caggtctggc agaaagtggc caggttctga cgagcgttgc catcaagata taattcatca
      481 ttgataatct ggaatgcgac atcatcgcgc atttcgtgca gcggaaatcg ttttgactcc
      541 gcgatagtag aaatggcctt tgcgccaaaa cgtgaatcga gtagttctga gcggaaatcc
      601 gttaacagct tctggtccat ttcgaactcc ttaaatttat ttgaaggcaa taaaaaagta
      661 ggatttatcc gcaatggaag cat
//
LOCUS       DAZGSP010000363          673 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZGSP010000363 DAZGSP010000000
VERSION     DAZGSP010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.367"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            367..561
                     /locus_tag="ACHK74_005439"
     CDS             367..561
                     /locus_tag="ACHK74_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224055.1"
                     /translation="MPENNTRKPDKSATVHIDAGTMEKIERYQQFIKDNHPGMPVPTK
                     GQITRSAVEYWYRATLGVWL"
BASE COUNT          215 a          136 c          148 g          174 t
ORIGIN      
        1 ttttgatttt tatggcacca tcaccaccac tataagctat tgatatgttg agaaataaaa
       61 atttttagtg tcgaatcagg ttgttttttg ttcatcaccg gaacgttccc gaaaacattt
      121 acaaaaaaca ggcgcaaaaa aagcgccccc gattgctgtt accggaggcg cttttacacg
      181 acaaaggagt ttttatcgcc aggatgacga gtcttaatac tgcttcaatg gcaaaaatgc
      241 gtcaataact ttgcctttct gagaataatc agaaaaatca taatctgatt ttcaggtata
      301 aaatgattta tctattactt ttatcgatca ataatgatgc ccgttaatca aaacggaggc
      361 ggatttatgc cagagaacaa caccagaaag ccggataaaa gtgccacggt acacatagac
      421 gccggaacta tggagaagat cgaacgctat cagcagttca tcaaagataa tcacccgggt
      481 atgccagtac ccacgaaagg acaaatcaca cgcagcgcgg ttgaatactg gtacagggca
      541 acgttagggg tctggctatg aaaacatggt tttccattaa ggctatggca gatgttgtct
      601 atgtgcgcat ttatgacgag atcggcgggt acggtgtaaa agcatcggca cttactgacg
      661 agatcaacgc gtg
//
LOCUS       DAZGSP010000364          663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZGSP010000364 DAZGSP010000000
VERSION     DAZGSP010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.368"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>663
                     /locus_tag="ACHK74_005440"
     rRNA            <1..>663
                     /locus_tag="ACHK74_005440"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          154 a          146 c          222 g          141 t
ORIGIN      
        1 tggtgggtag tttgactggg gcggtctcct cctaaagagt aacggaggag cacgaaggtt
       61 ggctaatcct ggtcggacat caggaggtta gtgcaatggc ataagccagc ttgactgcga
      121 gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga tccggtggtt ctgaatggaa
      181 gggccatcgc tcaacggata aaaggtactc cggggataac aggctgatac cgcccaagag
      241 ttcatatcga cggcggtgtt tggcacctcg atgtcggctc atcacatcct ggggctgaag
      301 taggtcccaa gggtatggct gttcgccatt taaagtggta cgcgagctgg gtttagaacg
      361 tcgtgagaca gttcggtccc tatctgccgt gggcgctgga gaactgaggg gggctgctcc
      421 tagtacgaga ggaccggagt ggacgcatca ctggtgttcg ggttgtcatg ccaatggcac
      481 tgcccggtag ctaaatgcgg aagagataag tgctgaaagc atctaagcac gaaacttgcc
      541 ccgagatgag ttctccctga ctccttgaga gtcctgaagg aacgttgaag acgacgacgt
      601 tgataggccg ggtgtgtaag cgcagcgatg cgttgagcta accggtacta atgaaccgtg
      661 agg
//
LOCUS       DAZGSP010000365          658 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZGSP010000365 DAZGSP010000000
VERSION     DAZGSP010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.369"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            61..>658
                     /locus_tag="ACHK74_005441"
     CDS             61..>658
                     /locus_tag="ACHK74_005441"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP0224056.1"
                     /translation="MLSWYNRVTDALTDICRQEHFYFRPVAVGFCLTSVAARPGNLWQ
                     QQLTSLTVLPPPAGVSSQALPLPDILLSGIPRHRGLSHWHRLCLQTGAIVGVFLLLAM
                     LASFMNNQRLVRSVGEHLTAWHRMRGYSSESKKTALQRLQADSQLLNDWQQRGVPQRY
                     GLGLYQGMWLIPPVERATGEWIAPLPPRPVIQKVTQTPK"
BASE COUNT          128 a          178 c          193 g          159 t
ORIGIN      
        1 cgggagtgtg atgcagcgtc caggctccgg tgcgtcagtg aactgttatg gctgaatgcc
       61 atgttgtcct ggtacaaccg ggttactgat gccctgacag atatttgccg gcaggagcac
      121 ttttatttcc ggccggttgc cgttggattc tgtctgacgt cggttgcggc ccggcccggt
      181 aatctctggc aacaacaact gacgtcactc actgtactgc caccgcctgc cggcgtgtca
      241 tcacaggcac tgccgttgcc ggatattctg ctgtccggta ttccccgtca tcgtggactc
      301 agtcactggc acagactttg tctccagacg ggggcgattg tcggcgtttt tcttctgctg
      361 gctatgctgg cgtcttttat gaataaccag cgactggttc gcagcgtggg ggaacacctg
      421 acggcctggc accggatgcg cggatattca tctgaatcga aaaaaactgc actgcaacgt
      481 cttcaggctg acagccagtt actgaacgac tggcagcaac gtggtgtgcc gcagcgttac
      541 ggactgggat tgtatcaggg aatgtggctg ataccgccgg ttgagcgagc gactggtgag
      601 tggattgcac cactgcctcc ccgtccggta atacagaaag ttactcagac gccgaaaa
//
LOCUS       DAZGSP010000366          658 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZGSP010000366 DAZGSP010000000
VERSION     DAZGSP010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.370"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>658)
                     /locus_tag="ACHK74_005442"
     CDS             complement(<1..>658)
                     /locus_tag="ACHK74_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP0224057.1"
                     /translation="LACDAASRLRCVSELLWLNAMLPWYNRVTDALTDICRQEHLYFR
                     PVAVGFCLTSVAARPGNIWQQQLTSLTALPPSAGVSSQVLPLPDILLSGIPRHRGLSH
                     WHRLCRQTGLIVGVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRGYSSELKTTALQR
                     LQADSQLLNDWQQHGVPQRYGLGLYQGMWLIPPVERATGEWVAPLPPRPVIQKVTQTP
                     K"
BASE COUNT          161 a          191 c          179 g          127 t
ORIGIN      
        1 ttttcggcgt ctgagtaact ttctgtatca ccggacgggg aggcagtggt gcaacccact
       61 caccagtcgc tcgctcaacc ggcggtatca gccacattcc ctgatacaat cccagtccgt
      121 aacgctgcgg cacaccatgt tgctgccagt cgttcagtaa ctggctgtca gcctgaagac
      181 gttgcagtgc agttgttttc aattcagatg aatatccgcg catccggtgc caggccgtca
      241 ggtgttcccc cacactgcga accagtcgct ggttattcat aaaagacgcc agcatagcca
      301 gcagaagaaa aacaccgaca atcaaccccg tctggcgaca aagtctgtgc cagtgactga
      361 gtcctcgatg acgggggata ccggacagca gaatatccgg caacggcagt acctgtgatg
      421 acacgccggc agacggtggc agtgcagtga gggacgtcag ttgttgttgc cagatattac
      481 cgggtcgggc agcaaccgac gtcagacaga atccgacggc aaccggccgg aaataaaggt
      541 gctcctgccg gcaaatatct gtcagggcat cagtaacccg gttgtaccag ggcaacatgg
      601 cattcagcca taacagttca ctgacgcacc ggagcctgga cgctgcatca cacgccag
//
LOCUS       DAZGSP010000367          657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZGSP010000367 DAZGSP010000000
VERSION     DAZGSP010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.371"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>657
                     /gene="yfdF"
                     /locus_tag="ACHK74_005443"
     CDS             <1..>657
                     /gene="yfdF"
                     /locus_tag="ACHK74_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFP0224058.1"
                     /translation="GSNDTDHITIGIDNQEPIELPANYKDKELVRTIINDNIVEKTHD
                     INNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLFTWYGKDKKG
                     DSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARNASAWFESSK
                     AIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKFRRDNVKEII
                     "
BASE COUNT          246 a          114 c          131 g          166 t
ORIGIN      
        1 ttggctccaa tgatacagac catattacta ttggtatcga caaccaagaa ccaatagaat
       61 tacctgcgaa ctataaagac aaagaactcg tccgtactat cattaatgac aacatagttg
      121 agaagactca tgatatcaat aacaaggaaa tgatcttcag cgcattaaaa gaaatatatg
      181 atggagatcc tggttttatc ttcgataaga tatcacacaa actcagacat acggtaacgg
      241 aatttgatga gagcgggaat agcgaaccaa cggacttatt tacctggtac ggtaaagata
      301 aaaaaggcga ctctctcgct attgtaatta aaaataaaaa cggaaatgat tacttatctc
      361 tcggttacta cgatcaggac gactaccaca ttcaaagagg aattcgtatt aatggtgata
      421 gtctcaccca atattgtagt gaaaaagcca ggaatgcttc agcgtggttt gaaagcagta
      481 aagctatcat ggcagaatca tttgcaacag gttccgatca tcaggttgta aacgagctca
      541 acggggaaag actgagagaa cctaacgaag tttttaaacg tttaggtcga gcaataagat
      601 ataattttca agtggacgat gcaaaattta gacgcgacaa tgtaaaagaa ataattt
//
LOCUS       DAZGSP010000368          655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZGSP010000368 DAZGSP010000000
VERSION     DAZGSP010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.372"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            102..>655
                     /locus_tag="ACHK74_005444"
     CDS             102..>655
                     /locus_tag="ACHK74_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP0224059.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWL"
BASE COUNT          132 a          194 c          201 g          128 t
ORIGIN      
        1 attgttctta tctgacagat atctcactta aggctttctt ataaatctgt agggttttgc
       61 ctggaagcag acaaataacc cgataaaaca aggatgagca gatgagcgga aaaccggcag
      121 cgcgtcaggg cgacatgacg cagtatggcg gtagcattgt ccagggttca gccggggtgc
      181 gcatcggtgc ccccaccggc gtggcctgtt cggtgtgccc cggcggggtg acgtccggcc
      241 atccggtcaa tccgctgctc ggtgcaaagg tccttcccgg tgaaaccgac atcgccctgc
      301 ccgccccgct gccgttcatc ctctcccgca cctacagcag ttaccggaca aaaacgcccg
      361 cgccggtggg gagcctcggc cccggctgga aaatgcctgc ggatatccgc ttacagctgc
      421 gcgataacac actgatactc agtgataacg gcggcagaag cctgtatttt gagcacctgt
      481 ttcccggtga ggacggttac agccgcagcg agtcactgtg gctggtgcgc ggcggcgtgg
      541 cgaaactgga tgaaggtcac cggctggccg cactctggca ggcgctgccg gaagaactcc
      601 gcttaagtcc gcatcgttat ctggcgacaa acagtccgca ggggccgtgg tggct
//
LOCUS       DAZGSP010000369          594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZGSP010000369 DAZGSP010000000
VERSION     DAZGSP010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.373"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            29..520
                     /gene="ygeG"
                     /locus_tag="ACHK74_005445"
     CDS             29..520
                     /gene="ygeG"
                     /locus_tag="ACHK74_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP0224060.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCHPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCKIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
BASE COUNT          220 a           96 c          101 g          177 t
ORIGIN      
        1 atcacgagac acatcacaga ggaatattat gagcacagaa acaattgaaa tattcaataa
       61 tagtgatgaa tgggcaaatc aactaaaaca cgcattatcg aaaggagaaa atctggcatt
      121 actacatggt ttaactcctg atatccttga tagaatatat gcatatgcat tcgactacca
      181 tgaaaagggt aatataacgg acgcagaaat ttattataaa tttttgtgca tttatgcgtt
      241 cgaaaatcat gagtatctaa aagattttgc atcagtatgt caccccaaaa agaaatatca
      301 acaagcatat gacctttaca aactaagtta caattactcc ccgtatgatg actattcagt
      361 tatttatcgt atgggtcaat gtaagattgg ggctaaaaat atcgataacg caatgcaatg
      421 tttctatcac attattaaca attgtgagga tgatagtgtt aagagtaaag cgcaggcata
      481 tattgaactc ttaaacgata attcagaaga taatggctaa gcattacaat catttggtta
      541 aatttgctgg ttatagtgca gttaaccact ataaccagca cctcttatgt tttc
//
LOCUS       DAZGSP010000370          578 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZGSP010000370 DAZGSP010000000
VERSION     DAZGSP010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.374"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>578
                     /locus_tag="ACHK74_005446"
     CDS             <1..>578
                     /locus_tag="ACHK74_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFP0224061.1"
                     /translation="TALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDE
                     LPMKPNGKHYSPAEFRKKCLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNI
                     YYAPELRDEHGRKVLISYDIHDAERITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQ
                     QQRLKGMRKRAEEKIRDAEDEVVNVLEHKPQEP"
BASE COUNT          157 a          138 c          173 g          110 t
ORIGIN      
        1 accgccctgc aacagggcaa acggctgaac gcccggcaac agtcagcgat gcgtgattta
       61 ccgtcctggt ctgaactgat tgatgccatt cgtgatggtg ttgagtggta caacaaccga
      121 ccgcacgatg aattaccgat gaagccgaac ggtaaacatt acagcccggc ggagttcaga
      181 aaaaaatgcc tggcggaaga ggacacggaa attgaatggc tgtccgatgt ggaattgcgg
      241 gacatgttcc ggccgatggt ggaacgccct gtaagacgct gtgaaatacg ctggctgaat
      301 aatatttact acgcgcccga gctgcgtgat gagcatggcc gcaaggtgct tatcagctat
      361 gacattcatg atgccgaacg aattaccgta cgtcgcctgg atggcagcgt gatttgcgag
      421 gcggtatggg acggtaataa acgcgaagcc ttcccggtta gcgcggaata ctacaaacag
      481 cagcaacgcc ttaaaggcat gcgtaaacgc gcagaggaaa aaatccgtga tgccgaggat
      541 gaggttgtca acgtgctgga gcacaagccg caggagcc
//
LOCUS       DAZGSP010000371          578 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZGSP010000371 DAZGSP010000000
VERSION     DAZGSP010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.375"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>578
                     /locus_tag="ACHK74_005447"
     CDS             <1..>578
                     /locus_tag="ACHK74_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFP0224062.1"
                     /translation="TALQQDKRLNARQQSAMRDLPSWPELIDAIRDGVEWYNNRPHSG
                     LPVKPNGKHYSPAEYRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNI
                     YYAPELRDEHGRKVLISYDIHDAERITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQ
                     QQRLKGMRKRAEEKIRDAEDEVVNVLEHKPQEP"
BASE COUNT          153 a          144 c          179 g          102 t
ORIGIN      
        1 actgccctgc aacaggacaa acggctgaac gcccggcagc agtcagcgat gcgtgattta
       61 ccgtcctggc ctgaactgat tgatgccatc cgtgatggtg tcgagtggta caacaaccgg
      121 ccgcacagcg gattgccggt gaaaccgaac ggtaagcatt acagcccggc ggaatacagg
      181 aaaaaacgcc tggcagaaga ggacacggaa attgaatggc tgtccgatgt tgagctgcgg
      241 gatatgttcc ggccgatggt ggaacgcccg gtaagacgct gtgaaatacg ctggctgaat
      301 aatatttact acgcgcccga gctgcgtgat gagcatggcc gtaaggtgct tatcagctat
      361 gacattcatg atgccgaacg aattaccgtg cgtcgcctgg atggcagcgt gatttgcgag
      421 gcggtatggg acggcaataa acgcgaagcc ttcccggtca gcgcggaata ctacaaacag
      481 cagcaacgcc ttaaaggcat gcgtaaacgc gcagaggaaa aaatccgcga tgccgaggat
      541 gaggtggtca acgtgctgga gcacaagccg caggagcc
//
LOCUS       DAZGSP010000372          560 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZGSP010000372 DAZGSP010000000
VERSION     DAZGSP010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.376"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>560
                     /locus_tag="ACHK74_005448"
     CDS             <1..>560
                     /locus_tag="ACHK74_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HFP0224063.1"
                     /translation="MAETYAGRTGRSKQEITAMMEAETWMDGNECKANGFADEVIPAI
                     TAMARIESKRIGDFSNMPEKIKSMISQKTTSGEQERLNGIRELFGTFNGRYNDLAISC
                     LADSECSVENARERLLLAMGKESTPTNKTTPANLYYAYTDNGNITGDAMRQGLNARLG
                     HERAERGNPYAMMSLFDMAQASLTHR"
BASE COUNT          175 a          133 c          142 g          110 t
ORIGIN      
        1 cgatggcgga aacctacgcc ggaagaacgg gcaggagcaa acaggaaatc accgccatga
       61 tggaggcgga aacctggatg gatggcaatg agtgtaaggc taacggcttc gcagatgagg
      121 ttatacccgc gattacagca atggcccgaa ttgaatcaaa acgaatcgga gatttttcaa
      181 atatgccgga aaaaattaaa agcatgatca gccagaaaac taccagtggc gaacaggaac
      241 gacttaacgg catccgtgaa ttgtttggca cgttcaacgg aagatataac gacctggcta
      301 taagttgtct tgctgattca gaatgtagcg ttgagaatgc acgcgaacgc cttttactcg
      361 ctatgggtaa agaatcaacg ccaacaaaca aaaccacccc cgcaaatctt tactacgcgt
      421 acacggataa cggcaacata accggcgatg caatgcgcca ggggcttaat gcgcgtcttg
      481 gtcacgaacg ggccgaacgc ggtaatcctt acgccatgat gagccttttc gatatggcac
      541 aggcatcatt aacccatcgt
//
LOCUS       DAZGSP010000373          556 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZGSP010000373 DAZGSP010000000
VERSION     DAZGSP010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.377"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..88)
                     /locus_tag="ACHK74_005449"
     CDS             complement(<1..88)
                     /locus_tag="ACHK74_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0224064.1"
                     /translation="MSEDIPLPKINQRYRDDHGALVTVTSVEE"
     gene            complement(377..>556)
                     /locus_tag="ACHK74_005450"
     CDS             complement(377..>556)
                     /locus_tag="ACHK74_005450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224065.1"
                     /translation="EPIATEGLPLCTDLISPIIGRRSANSQYDRHIDRFNANYQIFIL
                     IHIAKSAFIKTYIQG"
BASE COUNT          137 a          109 c          106 g          204 t
ORIGIN      
        1 tttcctcaac actggtcacg gtgacaagcg cgccatgatc gtctctgtat cgctgattga
       61 tttttggtaa tggaatatct tctgacatag acttcagaac ggtaaccgtt cactaaattt
      121 tcatgtggta tcactgaatc aggagacgac gtttcctgat gtgcgttgtt aattttctgt
      181 ttttaaaaaa tattttctct ctcttcttta ttttccgtgt cttttattgc tctcctcttt
      241 catgtccttc tctttttctg atatgccctt aaatgctgcc gaatcatcac tcctcgtgaa
      301 tgtcagtgct ggtaatcatt cggtataacc atctgtatca aaagcactaa ccggaataat
      361 tttctgcatt tttgtattac ccctggatat aagttttgat aaatgctgat tttgcgatat
      421 gtatcagaat aaagatctgg taattggcat taaaacgatc gatatgtctg tcatattgag
      481 aatttgcaga tcttcgtcct ataatgggtg aaattaaatc agtgcacaag gggaggcctt
      541 ctgtggcgat gggctc
//
LOCUS       DAZGSP010000374          531 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZGSP010000374 DAZGSP010000000
VERSION     DAZGSP010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.378"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..414)
                     /locus_tag="ACHK74_005451"
     CDS             complement(<1..414)
                     /locus_tag="ACHK74_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP0224066.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSES"
BASE COUNT          117 a          164 c          136 g          114 t
ORIGIN      
        1 tgactcgctg cggctgtaca ccgcctcccc cggcagcagc ggctcaaagt gaatgctccg
       61 cccgccgttg tcgttgagta ccagtgcatc atcgcgtagc tgtaagcgga tatcagaagg
      121 cgctttccag ccggggccaa acactcccac cggcgcgggt gtcctggtcc ggtagctgct
      181 gtaggtgcgg gagagaatga acggcagcgg gccgggcagc gcaaggtccg tctcgccggg
      241 cagcaccttc gcccccagca gcggatttac cgggttgccc gaagtcatcc cgcccggaca
      301 caccgagcac gccacgccgg tgggcgcgcc aattcttaca cctgccgaac cctggacaat
      361 aggaccgcca tactgagtca tatctccctg acgcgccgct ggttttccgc tcatataaag
      421 tgccctttta gttattgttc atatccctgt gttagcaaca aaaaaaataa aatgacacac
      481 aagaccagct aatgtattta tatatttttc acataaactt atttgttctc a
//
LOCUS       DAZGSP010000375          502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZGSP010000375 DAZGSP010000000
VERSION     DAZGSP010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.379"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>502
                     /locus_tag="ACHK74_005452"
     CDS             <1..>502
                     /locus_tag="ACHK74_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP0224067.1"
                     /translation="VPAGSAEGDTVTLTVTKPDGKTETVSHKLTAEEVKDGKANVGIP
                     ADKVTQDGEYTVKAEITDPAGNTSGQGKATQFGVDTVAPSEPALKAENDGSVSATLPD
                     GANKGDKVEVTFTDEEGNEQKVTLEKGDGNWSSDKPELIPDSTDNKVTVPADKVKNNT
                     EVTATAK"
BASE COUNT          162 a          104 c          154 g           82 t
ORIGIN      
        1 gttccggcag gcagtgctga aggtgatact gtgacgctga cggtcaccaa accggacggg
       61 aaaacagaaa cggtcagtca caaacttact gccgaagaag taaaagatgg taaagcgaat
      121 gtgggcattc ctgcggataa agtgacgcag gatggggaat acacagttaa ggctgaaatc
      181 acagacccgg caggcaacac cagtggtcag ggtaaagcta cccagtttgg cgtggatact
      241 gtagctccgt cagaaccagc actgaaggct gagaatgatg gttctgtatc agcgactctg
      301 ccagatggtg cgaacaaagg tgacaaggtg gaagtgacct ttacggatga agagggtaat
      361 gagcagaaag tcacgctgga gaaaggggat ggcaactgga gttccgacaa gcctgaactg
      421 atcccggaca gcaccgataa taaagtgaca gttccggcag acaaagtgaa gaacaacaca
      481 gaggtgacgg caacagcgaa ag
//
LOCUS       DAZGSP010000376          492 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZGSP010000376 DAZGSP010000000
VERSION     DAZGSP010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.380"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>492
                     /locus_tag="ACHK74_005453"
     CDS             <1..>492
                     /locus_tag="ACHK74_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFP0224068.1"
                     /translation="ELEAVAAEILKRAEASAASAEEAKKRAENARGPKGDKGDTGPQG
                     IPGPKGDTGERGPKGERGETGPQGLQGVKGERGEKGEKGEPGGPDATTAQKGIVQLSS
                     ATDSDDETKAATPKAVKAAMDKADGCLEKAKNGDDIPDKVKFLNTVGAARVYGRDIHT
                     GAG"
BASE COUNT          144 a           97 c          200 g           51 t
ORIGIN      
        1 agagctggaa gcggtggccg cggaaatcct gaaacgtgcg gaagcgtcag cggcgagtgc
       61 agaggaagcg aagaaacggg cagagaatgc gaggggaccg aagggggata agggagacac
      121 ggggccacag ggtattcccg ggccaaaagg cgataccggc gagcgggggc caaagggtga
      181 gcgtggtgag acaggaccac aggggcttca gggtgtgaaa ggtgagcggg gagagaaggg
      241 cgaaaaaggt gagccgggag ggccggatgc gacgacggca cagaagggaa ttgtgcagtt
      301 aagcagcgca acggacagtg atgatgaaac gaaggcagcc accccgaaag cggtgaaagc
      361 ggcaatggac aaagcggacg gatgcctgga gaaagcgaaa aacggtgacg atatcccgga
      421 taaggtgaag tttctgaaca ccgtgggagc agccagagta tacgggcggg acattcatac
      481 gggggccggt ga
//
LOCUS       DAZGSP010000377          485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZGSP010000377 DAZGSP010000000
VERSION     DAZGSP010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.381"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>485
                     /locus_tag="ACHK74_005454"
     rRNA            <1..>485
                     /locus_tag="ACHK74_005454"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          121 a          110 c          163 g           91 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaacc
//
LOCUS       DAZGSP010000378          483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZGSP010000378 DAZGSP010000000
VERSION     DAZGSP010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.382"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..474
                     /locus_tag="ACHK74_005455"
     CDS             <1..474
                     /locus_tag="ACHK74_005455"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP0224069.1"
                     /translation="FFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIK
                     TVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGDNPEYA
                     IPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP"
BASE COUNT          145 a           90 c          130 g          118 t
ORIGIN      
        1 ttttttaatg aactgacagt tcaatcagca tcagccaaaa aaacgcctta ttgcgtcatg
       61 attatggata tcgaccactt caaaaaagtc aacgacacct gggggcatcc ggttggcgat
      121 caggtgataa aaacagtggt gaatatcatt gggaaaagca tacgaccaga tgacctttta
      181 gcgcgcgtcg gcggcgaaga gtttggcgtc ttgctgacgg acatcgatac tgaacgtgcg
      241 aaagctttag cggaaaggat tcgggaaaat gttgagcgtt taactggcga taatcctgaa
      301 tacgctatac cacaaaaagt gacgattagt attggcgctg ttgtgactca ggaaaatgcg
      361 ttaaacccga atgagatcta tcgactggct gataacgcac tttacgaggc gaaagagacc
      421 gggcgtaata aggtggttgt gagggatgtg gtgaattttt gtgagtcacc ataaagcggc
      481 att
//
LOCUS       DAZGSP010000379          482 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZGSP010000379 DAZGSP010000000
VERSION     DAZGSP010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.383"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..482
                     /locus_tag="ACHK74_005456"
     rRNA            <1..482
                     /locus_tag="ACHK74_005456"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          118 c          142 g          103 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcacctcc
      481 tt
//
LOCUS       DAZGSP010000380          471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZGSP010000380 DAZGSP010000000
VERSION     DAZGSP010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.384"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(60..344)
                     /locus_tag="ACHK74_005457"
     CDS             complement(60..344)
                     /locus_tag="ACHK74_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP0224070.1"
                     /translation="MSEVKPLVTADAVKDALRSEDVRGALKQKVRQTFEAQIEADVDA
                     ILDTILGEQPGEQPEPEPQPEDITSENGDIQPESPVVDATEAQPEPGTML"
     gene            complement(370..>471)
                     /locus_tag="ACHK74_005458"
     CDS             complement(370..>471)
                     /locus_tag="ACHK74_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0224071.1"
                     /translation="VRDFWRRQIDKRAAEKISASQSAGSKPEEPITP"
BASE COUNT           83 a          130 c          119 g          139 t
ORIGIN      
        1 acggaagctc acaacatgaa aaaggccagc agtactttac tgatggccct gactcaccct
       61 tacagcatgg tgcccggttc gggttgtgct tcagtcgcat caaccaccgg tgattccggt
      121 tgaatatcgc cgttttctga ggtgatatct tccggttgcg gttccggctc tggctgttcg
      181 cctggctgct ctcccagtat cgtatccaga atcgcatcca catctgcttc tatctgcgct
      241 tcaaatgtct ggcggacttt ctgtttcagt gctccacgca catcttctga gcgcagcgcg
      301 tctttcactg catcagcagt gaccaggggt tttacttctg acatagggtt ttctcgttga
      361 aaggggttgt taaggagtaa tgggctcttc gggtttgctt ccggctgact gactggcgct
      421 gattttctct gcggcccttt tatcaatctg cctgcgccag aaatcgcgca c
//
LOCUS       DAZGSP010000381          459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZGSP010000381 DAZGSP010000000
VERSION     DAZGSP010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.385"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            11..>459
                     /locus_tag="ACHK74_005459"
     CDS             11..>459
                     /locus_tag="ACHK74_005459"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin"
                     /protein_id="HFP0224072.1"
                     /translation="MTSKTDGVADAPALTIPEVADSVANAAELKDGLQAEVKLPAGTV
                     EGAVITLTVTHPDKTTRTETHTVSKDEAADGTVSMVVPKGSVVDGQNSVSVSLTQGSN
                     PAKAGNKVEFVVDGQVPGDTNGDGVADVTPAVAIPEATDGVNAKELK"
BASE COUNT          119 a           94 c          152 g           94 t
ORIGIN      
        1 tccggtcact gtgacgtcaa aaactgatgg tgtggcagat gctccggccc tgaccattcc
       61 agaagtggct gatagtgtgg ccaatgcggc tgagctgaaa gatggtttac aggcagaagt
      121 caagttgccg gcaggaacag tggaaggtgc tgtcattaca ctgacagtga ctcatccgga
      181 taaaaccacc agaaccgaga cgcatactgt tagcaaagat gaggctgcag acggtactgt
      241 gtcaatggtt gttccgaagg gaagtgttgt tgacggtcag aacagtgtga gtgtcagcct
      301 gactcagggc agtaacccgg ctaaagcggg gaataaagtg gagtttgtgg ttgacgggca
      361 ggttccgggt gacaccaatg gtgatggtgt ggcggatgtc acaccggcag tggccattcc
      421 ggaagcgacg gacggtgtga atgccaaaga actgaagga
//
LOCUS       DAZGSP010000382          452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZGSP010000382 DAZGSP010000000
VERSION     DAZGSP010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.386"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          155 a           83 c           81 g          133 t
ORIGIN      
        1 aatatatttt atgtatttcg catggtgatg atttatacac ctgcactgaa tttttctgta
       61 tttccagaac tgaatcgata aacagaattg gtaataatga aagttaataa cataaaaaag
      121 aggttaccgc cttataatga taagagttca ttatccattc catcccgaca aaacctgcct
      181 gtgtataaaa aataaggacc atgtcacaaa acattacatt ccggcaagtt atgacagaat
      241 tactgaaaac cgttatctgt agtaattgta gtttctgata tatgctcatc cacccggaaa
      301 tacgacatgg tcctgactgg agttcacttc aaagtgtaat ttattattaa cacactgaga
      361 ctgtaaaacg gatggcgtgg atgcgtacac agtctgaaga gtataaaagg aacagctccg
      421 gattgttacc cgggattact atagttcacc ac
//
LOCUS       DAZGSP010000383          442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZGSP010000383 DAZGSP010000000
VERSION     DAZGSP010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.387"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>442
                     /locus_tag="ACHK74_005460"
     CDS             <1..>442
                     /locus_tag="ACHK74_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFP0224073.1"
                     /translation="GAFLSAIHHQIIQNQLDTETITVSYEFILCQHLIANTEFSYLAL
                     PENYNRLFLPNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIK
                     GYDQILNEKTAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQ"
BASE COUNT          163 a           80 c           78 g          121 t
ORIGIN      
        1 aggcgctttt ttatcggcta ttcatcatca gatcattcaa aatcaactgg atacagaaac
       61 aataactgtt agttatgaat ttatcctctg tcaacatctt atcgcaaata ccgagttcag
      121 ctatttggca ctccctgaaa attataaccg tttgttttta ccaaattcaa aaaaccaaac
      181 aaataatcgt ttcaaaacgt taaacagcaa agctattggc aggctacttg ctgctggtgg
      241 cgtatacaat gggaacattg aaggattcag agatactgcg gaaaaactgg gtggagatgc
      301 aataaaaggc tatgatcaaa tactaaatga aaaaacagcg ggcatagcga tagcaacagc
      361 atctattctt ttaacaaagc gttctaatgt tgatacatat acagaaataa atagttactt
      421 aggcaaactt agaggtcaac aa
//
LOCUS       DAZGSP010000384          441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZGSP010000384 DAZGSP010000000
VERSION     DAZGSP010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.388"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>441
                     /locus_tag="ACHK74_005461"
     CDS             <1..>441
                     /locus_tag="ACHK74_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFP0224074.1"
                     /translation="YITQDPIGLKGGWNLYTYPLSPVNSMDPLGLYEFKSKNIDDIGI
                     FALAMCNGESINENKEYGGLICKKQGEYLPMNPISSNDNDSVDLRNIKCPEGSERVGD
                     YHTHGFYSDDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMEKKE"
BASE COUNT          171 a           52 c           97 g          121 t
ORIGIN      
        1 ggtatatcac tcaggatccg attgggctga aggggggatg gaacctgtat acatatccgc
       61 tgagcccggt gaatagcatg gatccattag gattatatga atttaaatca aaaaatatag
      121 atgatattgg aatatttgca ttggcaatgt gtaatggaga atcaattaac gagaataaag
      181 aatatggtgg actaatatgt aagaagcaag gtgaatattt acccatgaat ccgataagtt
      241 caaatgataa tgatagtgta gacttgcgaa atataaaatg ccctgaaggt tcagagagag
      301 taggcgatta tcacactcac ggtttttact ctgacgataa aggaaataaa gtaacaaaag
      361 aaaatgatgt ttatgatagt ctaaattttt caagcaaaga tttaacgaat tcttatatga
      421 atggaatgga aaaaaaagaa t
//
LOCUS       DAZGSP010000385          437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZGSP010000385 DAZGSP010000000
VERSION     DAZGSP010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.389"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>437
                     /locus_tag="ACHK74_005462"
     CDS             <1..>437
                     /locus_tag="ACHK74_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFP0224075.1"
                     /translation="IPNRSWETLWMLSIGVIAAILFECAAKLIRSYLTDIAGKKADLI
                     ISSALLRRVMAVKMSDRPASSGSYASNLREYESVREFMTSATLLAFVDLPFILLFIGV
                     IGLVGGWLAVVPLTLVPLVILAGILLQRPLSRHINASMRESSQR"
BASE COUNT           84 a           98 c          130 g          125 t
ORIGIN      
        1 atacccaatc gctcctggga aacgttatgg atgctgagta tcggggtgat tgcagccatt
       61 ctgtttgagt gtgctgcaaa gttaatccgg agctatctga cggatattgc gggtaagaag
      121 gcagacctga ttatcagctc agcactgtta aggcgggtga tggcagtgaa aatgtcggac
      181 aggccggcgt cttccggttc atacgccagc aatttgcggg aatatgagtc tgtcagggag
      241 tttatgacca gcgccacgct gctggctttt gtggatttgc cgtttatcct gcttttcatc
      301 ggtgtcatcg ggcttgtggg gggctggctt gctgttgttc ctcttacact ggttccgctg
      361 gtgattctgg caggaatatt actgcagcga ccgctttcgc gtcatattaa tgcctccatg
      421 cgggaatcat cgcagcg
//
LOCUS       DAZGSP010000386          427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZGSP010000386 DAZGSP010000000
VERSION     DAZGSP010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.390"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>427
                     /locus_tag="ACHK74_005463"
     CDS             <1..>427
                     /locus_tag="ACHK74_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224076.1"
                     /translation="GKSASATIAVNAGKGGLTDAAGTTKTRLTYAPDGTLPAEELATM
                     NDGLSFEGDDGKIIHKKLNEKLTIKGGAGDNEDVTDSNIRVDANEKGDGLLVRMTRNL
                     RDLASAVFGTGGQITTVNDKGITITTPAAGGGQGNTVSLT"
BASE COUNT          127 a           85 c          118 g           97 t
ORIGIN      
        1 acggaaagtc tgcgtcagca accattgctg ttaatgcagg caaaggtgga ttaacagatg
       61 ctgccggtac cacgaagaca cgtctgactt atgctccaga tggaacatta ccggcagaag
      121 agctggcgac catgaatgac ggtctttctt ttgagggtga tgacggaaag attattcata
      181 aaaaactgaa tgaaaaactg accatcaaag gtggtgctgg tgataatgag gatgtaacag
      241 acagtaacat tcgtgttgat gcaaatgaaa agggagatgg tcttctcgtc cggatgacca
      301 gaaacctccg tgatttggcc agcgcggttt ttggtacagg tgggcagata acaacagtta
      361 atgataaagg catcacaatc accacaccgg ctgcgggcgg tggtcagggg aatacggttt
      421 cccttac
//
LOCUS       DAZGSP010000387          424 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZGSP010000387 DAZGSP010000000
VERSION     DAZGSP010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.391"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>424
                     /locus_tag="ACHK74_005464"
     CDS             <1..>424
                     /locus_tag="ACHK74_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224077.1"
                     /translation="GKSASATIAVNAGKGGLTDAAGTTKTRLTYTPDGKSPAEELATM
                     NDGLSFEGDDGKIIHKKLNEKLTIKGGAEKNAEVTDGNIRVDNDNGGLLVRMTRNLRD
                     LASAVFGTENGPTTRIDNAGITITTPAAGGGQGNTVSLT"
BASE COUNT          124 a           90 c          120 g           90 t
ORIGIN      
        1 gtggaaagtc tgcgtcagca accattgctg ttaatgcagg caaaggtgga ttaacagatg
       61 ctgccggtac cacgaagaca cgtctgactt ataccccgga tggaaaatca ccggcagaag
      121 agctggcgac catgaatgac ggtctttctt ttgagggtga tgacggaaag attattcata
      181 aaaaactgaa tgaaaaactg accatcaaag gtggtgcaga gaagaatgct gaagtgactg
      241 acggcaatat ccgggttgat aatgataatg gcgggcttct ggtgaggatg accagaaacc
      301 tccgtgattt ggccagcgcg gtttttggta cagaaaacgg cccgacaacc cggattgaca
      361 atgcaggcat cacaatcaca acacctgctg cgggcggtgg tcaggggaat acggtttccc
      421 ttac
//
LOCUS       DAZGSP010000388          419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZGSP010000388 DAZGSP010000000
VERSION     DAZGSP010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.392"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>419
                     /locus_tag="ACHK74_005465"
     CDS             <1..>419
                     /locus_tag="ACHK74_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224078.1"
                     /translation="QGAVSRGQNSIAMGNGAEVTRQAAAGSVAVGSHARAGRANTGTY
                     ALNGQAVAGRTGSDTAVVSFGQPGQERQLQSVAPGVLSANSTDAVNGSQLHATNRQVA
                     GNTQAINTLGNKFSQLSYRVEELNRDIRGVGASAAAM"
BASE COUNT           85 a          104 c          140 g           90 t
ORIGIN      
        1 cagggggccg tttcccgggg acaaaacagc attgccatgg gtaatggtgc tgaagtcacg
       61 cgtcaggctg ctgccggctc tgttgcagtt ggcagccatg cccgtgctgg ccgcgcgaat
      121 accgggactt atgccctgaa cggtcaggct gttgcgggac gtaccggaag tgatactgcg
      181 gtggtttctt ttggtcagcc gggacaggag cgtcagctgc agtcagtggc accgggtgtg
      241 ttgtctgcga attcaacgga tgccgtcaat ggcagtcagt tgcatgcaac taaccgtcag
      301 gttgccggaa atacacaggc gataaacact ctcgggaata aattcagtca gctgagctac
      361 cgggtggaag agcttaaccg ggatatccgt ggtgttggcg catcggctgc ggccatgag
//
LOCUS       DAZGSP010000389          398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZGSP010000389 DAZGSP010000000
VERSION     DAZGSP010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.393"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>398
                     /locus_tag="ACHK74_005466"
     CDS             <1..>398
                     /locus_tag="ACHK74_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="9-O-acetyl-N-acetylneuraminic acid deacetylase"
                     /protein_id="HFP0224079.1"
                     /translation="AKNPKNRLLAVVWMQGEGDAAVGTHAQHPGLFSAMVNQFRTELA
                     GQASQSTGGSASAVPWICGDTTYFWKQRHAEGYASVYGGYKGKESQNIFFVPLMTDEN
                     GANVQTNDPAEDPDLEAVGYYGSKWRNDQKT"
BASE COUNT          103 a           81 c          137 g           77 t
ORIGIN      
        1 gcgaagaacc cgaaaaaccg tctgcttgcg gtggtgtgga tgcagggaga aggtgatgcg
       61 gcagtgggga cgcatgcgca gcatccgggg ctgtttagtg cgatggtgaa tcagttcaga
      121 acggagcttg ccggtcaggc atcacagagc acaggaggca gcgcatcagc ggtaccgtgg
      181 atttgcgggg acacaacgta tttctggaag cagcgtcatg cggaagggta tgcatcagta
      241 tacggagggt ataagggtaa ggagtcgcag aacattttct ttgtaccgtt aatgacggat
      301 gagaatggtg cgaatgtgca gaccaatgac ccggcagaag acccggacct ggaagccgtc
      361 ggttattacg gttcaaagtg gcgtaatgac cagaagac
//
LOCUS       DAZGSP010000390          394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZGSP010000390 DAZGSP010000000
VERSION     DAZGSP010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.394"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>394
                     /locus_tag="ACHK74_005467"
     CDS             <1..>394
                     /locus_tag="ACHK74_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224080.1"
                     /translation="KYTRESCEKRFIYFLGSRKKVRFSTKKQPRYNPFTKNIVFTLLI
                     GKEERKLRIKYSFCTFTQNGVVKVTPNVRVTEKFLYIEYENGNLCVMSVHDFVNGHYI
                     DLGIHTEVHYVGYTRHPHRRPVDFIHRGL"
BASE COUNT          135 a           61 c           81 g          117 t
ORIGIN      
        1 aaaatatacg agggaaagct gtgaaaagag atttatttat ttcttaggtt caagaaaaaa
       61 agtgaggttt tcaaccaaga aacagcctag gtataaccca ttcacaaaaa atatagtatt
      121 cactctgttg attggcaaag aagagcgaaa gcttcggata aaatattcct tttgcacgtt
      181 tacgcagaat ggggtagtga aggtaactcc aaatgttaga gtaactgaga agtttctcta
      241 cattgaatat gaaaatggca atttatgtgt aatgtcagtt catgacttcg taaacggaca
      301 ttatattgat ttaggaattc atacagaagt gcattacgta ggatatacac gtcatccgca
      361 tcggcgccct gttgatttta ttcaccgtgg ctta
//
LOCUS       DAZGSP010000391          389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZGSP010000391 DAZGSP010000000
VERSION     DAZGSP010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.395"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            151..226
                     /locus_tag="ACHK74_005468"
     tRNA            151..226
                     /locus_tag="ACHK74_005468"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:185..187,aa:Glu,seq:ttc)
BASE COUNT          112 a           87 c           90 g          100 t
ORIGIN      
        1 agtgctcaca cagattgtct gatgaaaagt aaatagcaag gcgtttacgc gttgggagtg
       61 aggctgaaga gaataaggcc gttcgctttc tattaatgaa agctcaccct acacgaaaat
      121 atcacgcaac gcgtgataag caattttcgt gtccccttcg tctagaggcc caggacaccg
      181 ccctttcacg gcggtaacag gggttcgaat cccctagggg acgccacttg ctggtttgtg
      241 agtgaaagtc gccgacccca atatctcaaa acagactgtt aagtcttgtt tgagatattt
      301 gctctttaaa aatctggatc aagctgaaaa ttgaaacact gaacaacgaa agttgttcgt
      361 gagtctctca aattttcgca actctgaag
//
LOCUS       DAZGSP010000392          382 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZGSP010000392 DAZGSP010000000
VERSION     DAZGSP010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.396"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>382
                     /locus_tag="ACHK74_005469"
     CDS             <1..>382
                     /locus_tag="ACHK74_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP0224081.1"
                     /translation="AWFEPGQAYRETASGWYLRVDTPEQMRILAERLSYSRPALCGQV
                     VVMLGLLPEQHTSDEAFLSWLRNWQRAVMQCRQWLNTMPPVLVTLMVSGPSPQNTSLN
                     KHSAHWFTLTPDLSGMHIRLTEAGA"
BASE COUNT           83 a          100 c          118 g           81 t
ORIGIN      
        1 atgcgtggtt cgaacccggt caggcatacc gggaaacagc atcgggatgg tatcttcgtg
       61 tggatacgcc ggagcagatg agaatactgg cagagcggtt gtcgtacagc cgtccggccc
      121 tgtgtggcca ggtcgtggtc atgctgggac ttttaccgga gcagcatacc tcagatgaag
      181 ctttcctgtc gtggttgcgg aactggcagc gtgcagtcat gcagtgccgg cagtggctga
      241 ataccatgcc gccggtgctg gtgacattga tggtttcagg gccgtcacca caaaacacat
      301 ccttaaataa gcactctgca cactggttta cactgacacc ggacctgtcg ggaatgcaca
      361 tacgcctgac ggaagccgga gc
//
LOCUS       DAZGSP010000393          374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZGSP010000393 DAZGSP010000000
VERSION     DAZGSP010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.397"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            267..374
                     /locus_tag="ACHK74_005470"
                     /pseudo
     CDS             267..374
                     /locus_tag="ACHK74_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           88 a          106 c           70 g          110 t
ORIGIN      
        1 gaattattta gtacaaaaag tgaacttttg atgatctttc cctaatctca cataggatca
       61 gatctcgtct gatcgttccc cctgcgctca ctaccatggc tctttaccac agcagccggg
      121 gttgtttggg accgacttcc gacagcagat cccggtgggc ctgccaccat ctcttcacaa
      181 gcttatgctt attacatgca aaacgtcccg ttttgcatct ttgtgcctac ggcacaccga
      241 tgatctttcc ctgtgatctt tccctgaatc tgcccctgag aacctgaatc aaacgtctga
      301 ctcgcagaac catctgtttc tcgcgctctg tagaacttca tgaaaaagtc atcggtgctt
      361 ttatcgaaaa atac
//
LOCUS       DAZGSP010000394          370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZGSP010000394 DAZGSP010000000
VERSION     DAZGSP010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.398"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>370
                     /locus_tag="ACHK74_005471"
     CDS             <1..>370
                     /locus_tag="ACHK74_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224082.1"
                     /translation="FWEECQPGETQCTAVFNVVGENGQVTEAVLTQDGPVLENFTAGQ
                     SGTLAEGINNGIIWGGIAFGAGLAAIALAAGGGGGSSRHKRATEGEQKPVQPEGGQEA
                     GKTDVLSTGSTEAGSGSSSD"
BASE COUNT           90 a           72 c          128 g           80 t
ORIGIN      
        1 gattctggga agaatgccag ccgggtgaaa cacagtgtac agccgtgttt aatgttgtgg
       61 gtgaaaatgg tcaggttact gaagctgtcc tgacccagga tggtcctgta ctggagaatt
      121 tcactgcagg gcaaagtggc acactggctg aaggtatcaa taacgggatt atctgggggg
      181 ggattgcttt tggtgcaggg ctggcggcta ttgcactggc tgccggaggc ggtggtggaa
      241 gcagtcgtca taagcgtgca actgaaggtg agcagaagcc ggttcagcct gaaggcggtc
      301 aggaggctgg aaaaacagat gtattatcca caggctcaac tgaggcggga agtggaagct
      361 cttccgacca
//
LOCUS       DAZGSP010000395          365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZGSP010000395 DAZGSP010000000
VERSION     DAZGSP010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.399"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>365)
                     /locus_tag="ACHK74_005472"
     CDS             complement(<1..>365)
                     /locus_tag="ACHK74_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224083.1"
                     /translation="DEVLPAPLPPYRVLTGLVDRFGRTQTFHREAAGEFSGEITGVTD
                     GAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAVWLT
                     HDPEYPENLPAAPLVRYGW"
BASE COUNT           60 a          125 c          118 g           62 t
ORIGIN      
        1 tccagccata gcgcaccagc ggcgcggcag gtaaattctc cgggtattcc gggtcgtgcg
       61 tcagccacac ggcagacaga cggatgccgt tgtcccggcc atattcggtg taacccggca
      121 gggtatcagg aaaagcggac ggttccgtcc cgccggaaat ggcctgctgc cgggcttctt
      181 ctgcccgctg cgcctgcgtg gtcagtacca gccggaagtg acgcccggca ccatccgtca
      241 cgccggtgat ttcgccgctg aattcaccgg cggcttcgcg gtggaacgtc tgtgtgcgcc
      301 cgaagcggtc caccagcccg gtcagtaccc ggtacggcgg cagcggcgca ggcagcacct
      361 catcc
//
LOCUS       DAZGSP010000396          360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZGSP010000396 DAZGSP010000000
VERSION     DAZGSP010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.400"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>360
                     /locus_tag="ACHK74_005473"
     CDS             <1..>360
                     /locus_tag="ACHK74_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224084.1"
                     /translation="WQYDERGWLTDISHISEGHRVTVHYGYDEKGRLTGERQTVHHPQ
                     TEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGYLAGMKLGDTPLVEYTRDR
                     LHRETLRSFGRYELTTA"
BASE COUNT           82 a          108 c          117 g           53 t
ORIGIN      
        1 gtggcagtat gacgaacgcg gctggctgac agacatcagc catatcagcg aagggcaccg
       61 ggtgacggtg cattacgggt atgatgagaa aggccggctg accggtgagc gtcagacggt
      121 gcatcacccg cagacggaag cactgctctg gcagcatgag accagacatg cgtacaacgc
      181 gcaggggctg gcgaaccgct gtataccgga cagcctgccc gccgtggaat ggctgaccta
      241 cggcagcggt tacctggcag gcatgaaact cggcgacaca ccgctggtgg agtacacccg
      301 cgaccgcctg caccgggaaa cgctgcgcag cttcggccgt tatgaactca ccaccgctta
//
LOCUS       DAZGSP010000397          360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZGSP010000397 DAZGSP010000000
VERSION     DAZGSP010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.401"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>360
                     /locus_tag="ACHK74_005474"
     CDS             <1..>360
                     /locus_tag="ACHK74_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224085.1"
                     /translation="WQYDERGWLTDISHISEGHRVAVYYGYDEKGRLTGERQTVHHPE
                     TEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLAYGSGYLAGMKLGDTPLVDFTRDR
                     LHRETLRSFGRYELTTA"
BASE COUNT           80 a          107 c          117 g           56 t
ORIGIN      
        1 gtggcagtat gacgaacgcg gctggctgac agacatcagc catatcagcg aagggcaccg
       61 ggtggcggtg tattatggat atgatgagaa aggccgcctg accggtgagc gccagacggt
      121 gcatcacccg gagacggaag cactgctctg gcagcatgag accagacatg cgtacaacgc
      181 gcagggactg gcgaaccgct gtataccgga cagcctgccc gccgtggaat ggctggccta
      241 cggcagcggt tacctggcag gcatgaagct cggcgacaca ccgctggtgg atttcacccg
      301 cgaccgcctg caccgggaaa cgctgcgcag cttcggccgt tatgaactca ccaccgctta
//
LOCUS       DAZGSP010000398          345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZGSP010000398 DAZGSP010000000
VERSION     DAZGSP010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.402"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            118..>345
                     /locus_tag="ACHK74_005475"
     rRNA            118..>345
                     /locus_tag="ACHK74_005475"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          103 a           68 c           95 g           79 t
ORIGIN      
        1 tgtgtgggca ctcgaagata cggattctta acgtcgcaag acgaaaaatg aataccaagt
       61 ctcaagagtg aacacgtaat tcattacgaa gtttaattct ttgagcatca aacttttaaa
      121 ttgaagagtt tgatcatggc tcagattgaa cgctggcggc aggcctaaca catgcaagtc
      181 gaacggtaac aggaagaagc ttgcttcttt gctgacgagt ggcggacggg tgagtaatgt
      241 ctgggaaact gcctgatgga gggggataac tactggaaac ggtagctaat accgcataac
      301 gtcgcaagac caaagagggg gaccttcggg cctcttgcca tcgga
//
LOCUS       DAZGSP010000399          339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZGSP010000399 DAZGSP010000000
VERSION     DAZGSP010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.403"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>339
                     /locus_tag="ACHK74_005476"
     CDS             <1..>339
                     /locus_tag="ACHK74_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFP0224086.1"
                     /translation="NYTSKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRH
                     HPTEKPVTSLQPLIESFTHPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAG
                     QQRLAAVQRAM"
BASE COUNT           74 a          111 c           91 g           63 t
ORIGIN      
        1 aactacacat ccaaatctgc gtatgtcgcg taccgccacg aatgcgctta catcctggca
       61 aagggcagtc cggcccggcc ccgtaaccct ctgccggatg tgctgggctg gaaatattca
      121 ggcaatcgtc atcacccgac ggaaaagccc gttaccagcc tgcaaccact gattgagagc
      181 ttcacacatc cgaacgcgat tgtgctggac ccgtttgcag gcagcggctc aacctgcgtg
      241 gccgcccttc agtccggacg ccggtatatc ggtatcgagc tgcttgaaca gtatcaccgt
      301 gccggacagc aacgactggc cgccgtgcaa cgggccatg
//
LOCUS       DAZGSP010000400          333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZGSP010000400 DAZGSP010000000
VERSION     DAZGSP010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.404"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..120
                     /locus_tag="ACHK74_005477"
     CDS             <1..120
                     /locus_tag="ACHK74_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP0224087.1"
                     /translation="QAKITTSAARVMTIRLGRYQRVQEHYQRKEAQENGDYRW"
     gene            101..>333
                     /locus_tag="ACHK74_005478"
     CDS             101..>333
                     /locus_tag="ACHK74_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion lipoprotein TssJ"
                     /protein_id="HFP0224088.1"
                     /translation="MAIIAGKAGYGLIIALFSLSLSGCGLTQRVADGTVSATKSLFYR
                     QIKTLHLDIRAREAINTSAAGIPLSVVVRIYQL"
BASE COUNT           79 a           76 c           92 g           86 t
ORIGIN      
        1 caggcaaaga taacgacttc agcggcacgc gtaatgacca tccgtctggg gcgttatcag
       61 cgggttcagg aacattatca acgtaaggag gctcaggaaa atggcgatta tcgctggtaa
      121 ggctggttac ggtctgatta ttgcgttgtt ttccctgtcg ttatcagggt gtggcctgac
      181 gcaaagagtg gcagacggta cggtatctgc cacgaaatcc ctgttttacc gacagataaa
      241 aacacttcac cttgatattc gtgccaggga ggccattaac accagtgctg ccggtattcc
      301 cctgtcggtt gtggtgcgga tttatcagct taa
//
LOCUS       DAZGSP010000401          332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZGSP010000401 DAZGSP010000000
VERSION     DAZGSP010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.405"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..319
                     /locus_tag="ACHK74_005479"
     CDS             <1..319
                     /locus_tag="ACHK74_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0224089.1"
                     /translation="YIKTNIIEHDVIVHIWIYTPLTKVECDVFELLVKGYKIANVAQY
                     RARSLKTVSSQKHQVYKKLGIRNDVTFWIDIILSHHMRIVFCRNGKVIDTEKELLRMF
                     DSH"
BASE COUNT          125 a           42 c           60 g          105 t
ORIGIN      
        1 ttacataaaa actaatataa tagagcatga tgttatagtt catatatgga tatatacgcc
       61 tctgacaaaa gttgaatgcg atgtgttcga gttgctagta aaaggatata agatcgctaa
      121 tgtagctcaa tacagagctc gtagcctcaa aacagtttca tcacaaaaac atcaagtata
      181 taaaaaactt gggatccgta atgatgttac gttctggata gatataattt tatcacatca
      241 catgagaatc gttttttgtc gaaatgggaa agttattgat acagaaaaag agttattaag
      301 aatgtttgat agccattgat tgtaataaat tt
//
LOCUS       DAZGSP010000402          331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZGSP010000402 DAZGSP010000000
VERSION     DAZGSP010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.406"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            24..100
                     /locus_tag="ACHK74_005480"
     tRNA            24..100
                     /locus_tag="ACHK74_005480"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ACHK74_005481"
     tRNA            143..218
                     /locus_tag="ACHK74_005481"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           96 a           71 c           81 g           83 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaagtt ttgctcttta aaaatctgga
      301 tcaagctgaa aattgaaaca ctgaacaacg a
//
LOCUS       DAZGSP010000403          324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZGSP010000403 DAZGSP010000000
VERSION     DAZGSP010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.407"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>324
                     /locus_tag="ACHK74_005482"
     CDS             <1..>324
                     /locus_tag="ACHK74_005482"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001221415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFP0224090.1"
                     /translation="YTSIANNVSIGQGEHKVDAVSTSSMFYDNEFEDLTQNDCIIGND
                     VWLGTGVTVLRGVRIGDGAIIGANAVVTKNIPDYCIAVGVPAKVIKRRLPVNVTEKIK
                     ETKWW"
BASE COUNT          114 a           39 c           75 g           96 t
ORIGIN      
        1 attacacatc tattgcaaat aatgtctcaa ttggtcaagg tgaacataag gttgatgcag
       61 ttagtacaag ttcgatgttc tacgataatg agttcgaaga tttaacacaa aacgattgta
      121 ttataggtaa tgatgtgtgg ctcggtacag gagttactgt attacgagga gtaagaattg
      181 gtgatggtgc tattattggt gctaatgccg ttgtcacaaa aaatattcca gattattgta
      241 ttgcggtagg agtacctgcg aaagtaatca aaagaagatt accagtaaat gttactgaaa
      301 aaataaaaga gactaaatgg tgga
//
LOCUS       DAZGSP010000404          317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZGSP010000404 DAZGSP010000000
VERSION     DAZGSP010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.408"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>317)
                     /locus_tag="ACHK74_005483"
     CDS             complement(<1..>317)
                     /locus_tag="ACHK74_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0224091.1"
                     /translation="DNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTSTELVEDPDAI
                     LRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRHTPGDIIEI
                     CDN"
BASE COUNT           55 a          104 c           87 g           71 t
ORIGIN      
        1 tcgttatcac agatttcaat gatgtcaccg ggcgtgtgcc gcagcccctg tgacccgagc
       61 gtgaaatcca ccgtctgcgt ttccagcagt tctgtcttta tcacccacag tccggcacgg
      121 tgggcctgac cgcggctggt acagccaaac gcatccatct tcagcagatt gcgcccgtag
      181 cgcaggatgg cgtccgggtc ttccaccagt tcagtggaag tctgccagcc gttctgcggg
      241 tcggtgtaat tcacctccac cgccgtgtgc cggtccttca gggcactgaa gctgtagcgg
      301 aaccccacgc cgttatc
//
LOCUS       DAZGSP010000405          308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZGSP010000405 DAZGSP010000000
VERSION     DAZGSP010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.409"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>308)
                     /locus_tag="ACHK74_005484"
     CDS             complement(<1..>308)
                     /locus_tag="ACHK74_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0224092.1"
                     /translation="GGLPWLSEADRRLQIKSALPWWLVCRGEIHRFRCVPHLTGRRFE
                     HGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNLYLDNLADNGFYRVSPSCAQAG
                     "
BASE COUNT           64 a          107 c           85 g           52 t
ORIGIN      
        1 tcgcctgcct gtgcacagga cggagacacc cggtaaaagc cgttgtctgc caggttgtcc
       61 aggtacaggt tctgaccgtt gcgccaccag tcatcctcac gcacaaaatc cggcagcgtt
      121 atccccgcca gatggtatgc atcccggaac agggtgtaac agtccgtcac accgtgttca
      181 aagcgccgtc cggtcaggtg cggcacacag cggaaccggt gaatttcacc ccggcagacc
      241 agccaccagg gcagggcact ctttatctgc agccgccggt ccgcctcgct cagccagggc
      301 agaccacc
//
LOCUS       DAZGSP010000406          300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZGSP010000406 DAZGSP010000000
VERSION     DAZGSP010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.410"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>300
                     /locus_tag="ACHK74_005485"
     CDS             <1..>300
                     /locus_tag="ACHK74_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224093.1"
                     /translation="KEAEAALTQAKADNKITQQEHDDLAAKNDAVTAAKADAAKAVEG
                     LPAGDAKDGLNGRLAKVDGIDVPAVDENGNGKPDAEEAAEAVNAATAKVAEAEAK"
BASE COUNT           91 a           62 c          113 g           34 t
ORIGIN      
        1 aaaaagaagc agaagcggcg ctgactcagg cgaaggctga caataagatc acccagcagg
       61 agcatgatga tcttgcggcg aagaacgatg cggtaacggc ggcgaaagca gatgcggcga
      121 aagcggtgga aggtctgccg gccggagatg cgaaggacgg tctgaacggg cgtctggcta
      181 aagttgacgg tatcgatgta ccggcagtgg atgaaaacgg caacggcaag ccggatgcag
      241 aggaagcagc agaggcggtg aatgcagcga cagccaaggt agcagaagcg gaagcgaagg
//
LOCUS       DAZGSP010000407          294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZGSP010000407 DAZGSP010000000
VERSION     DAZGSP010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.412"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            97..218
                     /locus_tag="ACHK74_005486"
                     /pseudo
     CDS             97..218
                     /locus_tag="ACHK74_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           70 a           61 c           75 g           88 t
ORIGIN      
        1 atttgttagc cagatttctg ttctaatttt ggatatcaga atatgcatgt tatttttcga
       61 atcctccata actgatatat gtgctgttag tggagtacaa gaataagctc gcgttagcag
      121 tctgtcagta ctgcgacagc gttgtctggg agggctttac ccggtcaaaa cagcgttatt
      181 gtgcacccca tgtgacggat gaactgtgtt ctcagggggc attttactgt aaaaaccgtg
      241 ctgcggtcag ccgtaaccgt caggcgggtg aacgaaacag tcgcgccatt gctt
//
LOCUS       DAZGSP010000408          294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZGSP010000408 DAZGSP010000000
VERSION     DAZGSP010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.413"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>294)
                     /locus_tag="ACHK74_005487"
     CDS             complement(<1..>294)
                     /locus_tag="ACHK74_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224094.1"
                     /translation="PNPVEAVSPLPSPVSAPSPAPEASVTDVLTGENAGESGEYRDET
                     RWLLTGYRSTVNSSAVRDAGMLMSMGHRNFINEVNNLNKRMGDLRDINGEAGA"
BASE COUNT           58 a           98 c           73 g           65 t
ORIGIN      
        1 catgcaccgg cctcgccgtt gatgtcccgc aggtcaccca tacgtttgtt caggttgttg
       61 acctcattaa tgaagttacg gtgccccatt gacatcagca tccccgcatc cctgaccgcg
      121 gagctattaa ccgtactccg gtatcctgtc agcagccacc gggtctcatc acggtactca
      181 cctgattccc ccgcattctc tccggtcagt acatccgtca ctgacgcctc cggagccggt
      241 gacggagcag aaaccggtga agggagcggt gatacagctt ccacaggatt cggc
//
LOCUS       DAZGSP010000409          288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZGSP010000409 DAZGSP010000000
VERSION     DAZGSP010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.414"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>288
                     /locus_tag="ACHK74_005488"
     CDS             <1..>288
                     /locus_tag="ACHK74_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224095.1"
                     /translation="ADLKTAIDGVSSSMQGAGFALKDSDDKEVKQSLGQAIKVSGDSN
                     ITTTAITNGDKGLKISLNNDLTVGDKAPGSIKVNGADGKEAVSLNGQDGTIK"
BASE COUNT           87 a           56 c           85 g           60 t
ORIGIN      
        1 gcagacctga aaacggcaat tgatggtgtg tcctccagta tgcagggtgc aggtttcgca
       61 ctgaaagact ctgatgataa ggaagtgaaa cagtctcttg ggcaggcgat taaagtatcc
      121 ggggacagca atatcaccac cacagccatc accaatgggg ataaagggtt aaaaatatcc
      181 ctgaataatg acctgactgt gggggataag gctcccggca gcatcaaagt taacggggct
      241 gatggtaagg aggctgtgag tctgaatggt caggatggca cgattaaa
//
LOCUS       DAZGSP010000410          286 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZGSP010000410 DAZGSP010000000
VERSION     DAZGSP010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.415"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>286
                     /locus_tag="ACHK74_005489"
     CDS             <1..>286
                     /locus_tag="ACHK74_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP0224096.1"
                     /translation="IFGASFTNTRIKNLLCEDIMPGRWTTQLVNKHLGYRYTGVFKTL
                     ASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKELNKFMHDYDKTSSEMR"
BASE COUNT          107 a           51 c           49 g           79 t
ORIGIN      
        1 acatatttgg agcatcattc actaatacca gaataaaaaa tttgctctgt gaagatatta
       61 tgcctggtcg atggaccact caacttgtca ataagcactt aggttacaga tacacaggag
      121 tattcaaaac cctggcaagt attgatgaca aaccgtcccg tttcgaaatt ttgataccat
      181 tggtacaaac tttagttcgc gacaatgtaa agctaaataa tgatgtgtat aaagaactta
      241 ataagtttat gcacgattac gacaaaacat catcagaaat gagaaa
//
LOCUS       DAZGSP010000411          283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZGSP010000411 DAZGSP010000000
VERSION     DAZGSP010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.416"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            54..>283
                     /gene="dinJ"
                     /locus_tag="ACHK74_005490"
     CDS             54..>283
                     /gene="dinJ"
                     /locus_tag="ACHK74_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFP0224097.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDA"
BASE COUNT           84 a           69 c           68 g           62 t
ORIGIN      
        1 tcatttgagc tacaattcaa gctgaataaa tatacagcac aggagatacc ccaatggctg
       61 ctaacgcatt tgttcgcgcc cgaatcgatg aagatctgaa gaatcaggcg gcggacgtac
      121 tggccgggat ggggctgacc atctctgacc tggttcgcat aaccctcaca aaggtcgcgc
      181 gtgaaaaggc attgccgttt gatttacgcg agcctaatca attaaccatt caatcaatca
      241 aaaacagcga agctggcgtt gatgttcata aggccaaaga cgc
//
LOCUS       DAZGSP010000412          280 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZGSP010000412 DAZGSP010000000
VERSION     DAZGSP010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.417"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           74 a           51 c           59 g           96 t
ORIGIN      
        1 ctcctcctca tcagttcgta gcagagtgta ctcagcatgg caagttcagc ttctgatgat
       61 ttacagtgtg tattcaggag ggtaagaaat tgttcaaaaa catcagggtt tatactggtg
      121 ctttgatatt cgttactcat agtatttcct ttcaggtcat tattacttcc ggatttaaca
      181 tgatgtctgt aaccataaaa taatgaacgt gttccgggag tccttgccgt gtagtttccc
      241 gtaaaaatac tgttgtgcat tacatgtcat taggtggaaa
//
LOCUS       DAZGSP010000413          278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZGSP010000413 DAZGSP010000000
VERSION     DAZGSP010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.denovo.418"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>278
                     /locus_tag="ACHK74_005491"
     CDS             <1..>278
                     /locus_tag="ACHK74_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224098.1"
                     /translation="GNQITSVASGLNGTALADAGGDVLNNAANIGDLKNALRDSSAAL
                     VNTGFGLQADDGSKVNTSLGKHITVGGDGQNISTTVQNGSLTVRLADN"
BASE COUNT           69 a           63 c           89 g           57 t
ORIGIN      
        1 ggaaatcaga taaccagtgt ggccagtggt ctgaacggca ctgcgcttgc tgacgccggt
       61 ggagatgttc tgaataatgc ggccaatatc ggtgacctga aaaatgctct ccgtgacagc
      121 agtgcggcac tggtgaatac cggctttggt ctgcaggcgg atgacggaag caaggtgaac
      181 acgtctctcg ggaagcatat cacggttggt ggtgacgggc agaatatcag cacgactgtt
      241 cagaacggga gcctgacggt cagactcgct gataacat
//
LOCUS       DAZGSP010000414        18585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.guided.097, whole genome shotgun sequence.
ACCESSION   DAZGSP010000414 DAZGSP010000000
VERSION     DAZGSP010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.guided.097"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..238
                     /locus_tag="ACHK74_005492"
     CDS             <1..238
                     /locus_tag="ACHK74_005492"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0224099.1"
                     /translation="LGKVVLYQLSYSRVLLNFAFVIFAFLRRYGRRIIREIFPSATPQ
                     KSFFCVFISSAEIISKTFNLASKSRRRKWIPALT"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHK74_005493"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHK74_005493"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFP0224100.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="ACHK74_005494"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="ACHK74_005494"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFP0224101.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="asd"
                     /locus_tag="ACHK74_005495"
     CDS             2040..3008
                     /gene="asd"
                     /locus_tag="ACHK74_005495"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFP0224102.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGAFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="ACHK74_005496"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="ACHK74_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFP0224103.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VIEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFVNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6504..8005)
                     /gene="yjeM"
                     /locus_tag="ACHK74_005497"
                     /pseudo
     CDS             complement(6504..8005)
                     /gene="yjeM"
                     /locus_tag="ACHK74_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(8224..9201)
                     /gene="epmA"
                     /locus_tag="ACHK74_005498"
     CDS             complement(8224..9201)
                     /gene="epmA"
                     /locus_tag="ACHK74_005498"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFP0224104.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9526..11334
                     /gene="frdA"
                     /locus_tag="ACHK74_005499"
     CDS             9526..11334
                     /gene="frdA"
                     /locus_tag="ACHK74_005499"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFP0224105.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11327..12061
                     /gene="frdB"
                     /locus_tag="ACHK74_005500"
     CDS             11327..12061
                     /gene="frdB"
                     /locus_tag="ACHK74_005500"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFP0224106.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12072..12467
                     /gene="frdC"
                     /locus_tag="ACHK74_005501"
     CDS             12072..12467
                     /gene="frdC"
                     /locus_tag="ACHK74_005501"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFP0224107.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12478..12837
                     /gene="frdD"
                     /locus_tag="ACHK74_005502"
     CDS             12478..12837
                     /gene="frdD"
                     /locus_tag="ACHK74_005502"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFP0224108.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            12900..14033
                     /locus_tag="ACHK74_005503"
     CDS             12900..14033
                     /locus_tag="ACHK74_005503"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP0224109.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            14122..14655
                     /gene="blc"
                     /locus_tag="ACHK74_005504"
     CDS             14122..14655
                     /gene="blc"
                     /locus_tag="ACHK74_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP0224110.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14652..14969)
                     /gene="sugE"
                     /locus_tag="ACHK74_005505"
     CDS             complement(14652..14969)
                     /gene="sugE"
                     /locus_tag="ACHK74_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFP0224111.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15151..15297)
                     /gene="ecnB"
                     /locus_tag="ACHK74_005506"
     CDS             complement(15151..15297)
                     /gene="ecnB"
                     /locus_tag="ACHK74_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFP0224112.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15408..15533)
                     /gene="ecnA"
                     /locus_tag="ACHK74_005507"
     CDS             complement(15408..15533)
                     /gene="ecnA"
                     /locus_tag="ACHK74_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFP0224113.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(15585..16151)
                     /gene="efp"
                     /locus_tag="ACHK74_005508"
     CDS             complement(15585..16151)
                     /gene="efp"
                     /locus_tag="ACHK74_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP0224114.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16193..17221
                     /gene="epmB"
                     /locus_tag="ACHK74_005509"
     CDS             16193..17221
                     /gene="epmB"
                     /locus_tag="ACHK74_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFP0224115.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVVSGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            17611..18480
                     /locus_tag="ACHK74_005510"
     CDS             17611..18480
                     /locus_tag="ACHK74_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFP0224116.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT         4681 a         4835 c         4753 g         4316 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc caaaaaagtt ttttttgcgt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagttcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatcc agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagatagcgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaaacgacg gtcctgtccg atgctgttac
      481 cgcaaatcgg cgattttccc gcaggcaccc actgttttaa gaattcgagc gttgccagtt
      541 cagcttcccg atcgcccatc gtgctcgctt tcacgcgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcggactga tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg tgaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcagtgtt gacgcgtccg gatttctacg acggcgtgaa acctattgcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaat aagatagacc
     1381 tgctggacga cgaaggcatg gcgttcgtca acgagcagat ggatatctac cgcaacattg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctaaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg gccagtctgg cgtcggcaaa tccagcctgc
     1561 tgaacgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 gtctcggtca gcacaccacg accgccgcac gactgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaaggaaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgtagg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt cctggtcatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccttg ctggcaggca
     2401 actatctgat ggcggacctg ttccgcaacg gtgcgtttgt gaccacttac ctctccccgc
     2461 gtgactacca ccgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtgc
     2521 cgggcgatct cttctccgtt aaccatctca ccgctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatttgc cttttcgata ccgaatttgg cccaatggcg cagattctgg
     2641 tcggagcgac gattgttggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggtat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgcttaaagg ccaggaaatg ggtcgcttta aactcggttc caccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaacct gccccgcttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccagcccatt ggttgacgac aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcacccagg
     3121 aactggagca ggcaaaagcg gcgaaacccg cgcagccaga agtcatagag gcactccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaaa caatatcagc
     3241 aagttatcga taattacccg aaactctccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgagcc gcgcagcgtg tcgccgggaa tgtctaccga tgcgctgaat caggaaattc
     3361 tccaggtcag cagtcagttg ctggataaga gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattca ctgaatcaac tgccgcaaca gcaaaccgat gcccgccgtc
     3481 agttaaatga gatcgagcgc cgcctgggaa cgcttaccgg caatactccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactccg cacgcctgaa agcgctcgtt gatgaactgg
     3601 aactggcgca gctgtctgcc aataaccgtc aggaattagc gcgcttgcgc tcagagctgg
     3661 cggaaaaaga gagccagcaa ctggatgcgt atttgcaggc cttacgtaat cagttgaata
     3721 gccaacgtca gcttgaggcg gagcgggcgc tggaaagtac cgaattactg gcagaaaaca
     3781 gtgccgattt gccgaaagat atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcgg
     3841 cactgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa cctgctcggt gaagcgctgc gggcgcaggt ggcacggcta ccagaaatgc
     4021 caaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatctgct taataaacag ccgctgctac ggcaaatcca tcaggccgac ggtcagccgc
     4141 tgaccgccga gcaaaaccgt attctggaag cacaactgcg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacgctac tactggaact gaccaagctg aaagtctcca
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc aacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacgattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttacgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgttgccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggcgat ggcgtaacag ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgctac ttttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgggtttc ccgtgggatg cgctactacc tgatgagcat cgggcttatt gtgccactga
     4861 ttatggcgct gatgatgttc gttaacctcg acgaccgcga gttttccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg
     4981 ccgggatccc gctgtatctc aacaaagaag gcagcggcga caacattacc aaccatatgc
     5041 tgtggaacat gatgattggc gcgccactgg tcgccattct ggcgtcggcg gtgggttatc
     5101 tggcaacggc gcaggcactg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcacgtt atccgccgct ggatgctgat ccagcgccgc aggctggcgt
     5221 ttgaccgggc gaagcatcgc cgggcagaga tgttagcgca acgtgcgcgt ggcgaagagg
     5281 aagcgcatca tcacagtagc ccggaaggag caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat cagtgcgcaa tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttctgt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcacc tccacggtac agggcgtaga aagtctggag ccaattaccc
     5521 tcggtgcggt gctgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaacc
     5581 tgcccgcgct gctggaactg gcgattttgc agcacctgga tttaacgcca ggtacgggtt
     5641 acgccatcac caccatcacc aaatatctgc tgatgctgat tggcgggctg gtcggcttct
     5701 caatgattgg tattgagtgg tcgaaattgc agtggctggt tgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggaa attttcgcca actttatctc tggcctgatc atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtaa cgatccgcga tcttactggt agcgtgacaa
     5881 aaattaacac ccgcgccacc accatcagcg actgggaccg taaagagata atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgcg
     6001 tggtgttgac gattccggcc cctgccgatg ccaatagcga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtga tcgacaaccc ggcaccggaa gtcttcctgg
     6121 tggatctgca acaggggatt cagattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc gctacgccat gagatccacc agctaattct ggcaggcttc catgcccacg
     6241 gtatcgatat gccattcccg cccttccaga tgcgtctgga aagcctcaac ggtaagcaaa
     6301 cggggagaac attaacttct gcgggcaaag gtcgtcaggc gggaagtttg taatgcatga
     6361 agctgccaaa tgatgtcaac gtggctcatt tagcagctta acatcatctt cagttatcac
     6421 cggccctgga gagtatcatc catacgcatt acttcctgac aactcatcgc cggagagacg
     6481 atgagttgca ggatttcata tgatcagtcg agagctaact caggtttatt cgccatgcga
     6541 ctgcaatagt tctggtaaat cgccatcgct aacagcgaga agaagacagg gccgccaatc
     6601 atccacaatg tgctgtccca gtctccggct tcaaccacag gttgaatgat ggtgaagacg
     6661 ttcgcaaatg tcaccaccag tacgacaacc actgtcgcaa tcattgccga caaatgcgtt
     6721 ttgaaaatca caaacggtct gtcgagatcc tgacgtgctt taaagaacgg gaaagccagc
     6781 gcgaggaaca ggtaaggaag cgtcatagac acgttcgcca tcagcgtcag cttgttaaag
     6841 aacgccgatg cggtaccgcc accaaacgaa accagcagga tgaagacagt aaccaacccg
     6901 cactgcatcc acatggcgat agaaggcatc cccatcgcat tcaggcgcgt catcggttcc
     6961 ggccacaacg ctttcggcgt cccctggatg atggctttca gcggtgaata gcaaagcgta
     7021 aagaacgcac cggtataagc gaggaacatc gaaagtccgg taatacgcgc aaaccataca
     7081 cccagcgaca atgacgcttc aggtgacaaa tgcagtgcgt tacccagcgt caccccgagg
     7141 ctcttcatca gcacataggt aatattgccg aggttaacgg aaccattact taatacctgc
     7201 tgccagtttg tgctgacacc ccataaaaat attgccagcg aataaccgat tgaaataaca
     7261 atagcggcaa aaacaatacc tttggcaaag ttcttttctg gattttccgt tttatcgacc
     7321 agaccaccaa ccgcttcaat tccgccatag gcaaaaatag caaataccac aaacgatagc
     7381 attgccagac cggactgata acctgggttc ggtgatgcaa ggaaattaat atcctgcgcg
     7441 aaatgcccac catttaataa caaaatagta atgcttataa cagcaatact aaattcagac
     7501 acattactgc aataccgccc accgcagtaa tgcgggcaat tttattaatc ccctttgaag
     7561 caacgacggt gaccagaatc atccatgcta ctgccagtag accaaccacc tgcgtaggct
     7621 ccagtccggc aatacgccag tgctgggtca tgtcgctacc atagaggaat gttgagaacg
     7681 gtacccaaac tttcgcggag gtactcacca tccagatgat ataagaggaa aaccacataa
     7741 acgtaccaat gaaggcaaaa cgtggtccga cactattatt catccaggaa tagataccgc
     7801 cttcttcttt gcgataagca gctcccattt cagccatcat taaggcgaat ggaataaaga
     7861 ataacaatgc agaaaatata taaaagggaa tcgcactata acccattaag taataagccg
     7921 atgggctatt ggcaaatcca aatacggaag taaagatcat caatatgagt cctatcagac
     7981 tcatcttttt tatcgtgtga ggcatgaaac catccttcat ttcagcgcgt gtataacacg
     8041 tgcccactga actgcactgc gcaacattga gcaggcagcg taaaaaccaa tacagaattg
     8101 atacctggat gatatcaaat atccagtctt aaattgtggc ctttaacgga aaaatgaaaa
     8161 catatgctac agaatggcgc ttatcacgcc attcttcgct gttaattcag taatttttca
     8221 gaattatgcc cggtcaacgc taaaggcgat gacttcagcc agtgtctccg cgcccagcgc
     8281 caacatcacc agacgatcaa cacctaatgc cacgccggaa cagtcaggca taccgacttt
     8341 caaggcttca atcagattct ggtcaatggg gtgctgcggc aaaccgcgcg ccgcgcgctt
     8401 acggttatct tgttcaaagc gttgttgctg ctcacgggca tccgtcaatt catggaaacc
     8461 attcgccagc tcaatacctt tataataaac ctcaaagcgt tcagcgaccc gatgatcttc
     8521 ggtactgatt tgcgccagtg atgcctggct ggctggaaag tggtacacaa aggtcggttt
     8581 ttctttgcca atatttggct ctacgccaaa ggtaaacagc aattgtagca gcgtgtcgcg
     8641 gtcttcttcg gtatcagcaa cattgctcaa atccagtttc gctgcgactt cccgcagttg
     8701 cgttttgtcg gcagagagcg ggtcaatttc cagataacgc aagaaagctt gttgataaga
     8761 aaggctttct gctgccgggc agtccagcac ctgttgtaag agatcgtcca cctcgttcat
     8821 caaccggtac atatcatagt gcggtcgata ccactccagc atagtgaact cagggttgtg
     8881 ataacgcccc atctcttcat tacggaagct gcggcacagc tggaataccg gcccacaacc
     8941 ggcaaccagc aggcgtttca tatggtattc cgggctggtc attaaccaga gattcatccc
     9001 ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg accaaatgaa tatcggttac
     9061 cgtcgcctgg ctcatacaag gcgtctccac ctccagcact ccacgatcgg caaagaaacg
     9121 acggatctcc gccataatcg ccgcgcgttt taataagtta ggaatggatg cgctcggctg
     9181 ccaggatgcc gtttcgctca tagttaaatc tccagttttt gacaagggca cgaagtctac
     9241 tcgcaacgcg acggcgagac aaattttacg caggaatcaa acagcggtgg gcagtgacta
     9301 aaaaaagcac gatctgatgg tttagtaatt aaattaatca tcttcagtga taatttagcc
     9361 ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc agactatact
     9421 gttgtaccta taaaggagca gtggaatagc gttcgcagac cgtaactttc aggcacttac
     9481 cctgaagtac ggggctgtgg gataaaaaca atctggagga atgtcgtgca aacctttcaa
     9541 gccgatcttg ccattgtagg cgccggtggc gcgggattac gtgctgcaat tgctgccgcg
     9601 caggcaaatc caaatgcaaa aatcgcacta atctcaaaag tatacccgat gcgtagccat
     9661 accgttgctg cagaaggggg ctccgccgct gtcgcgcagg atcatgacag cttcgaatat
     9721 cactttcacg atacagtagc gggtggcgac tggttgtgtg agcaggatgt cgtggattat
     9781 ttcgtccacc actgcccaac cgaaatgacc caactggaac tgtgggggtg cccatggagc
     9841 cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat cgagcgcacc
     9901 tggttcgccg ccgataagac cggcttccat atgctgcaca cgctgttcca gacctctctg
     9961 caattcccgc agatccagcg ttttgacgaa catttcgtgc tggatattct ggttgatgat
    10021 ggtcatgttc gcggcctggt agcaatgaac atgatggaag gcacgctggt gcagatccgt
    10081 gctaacgcgg tcgttatggc tactggcggt gcgggtcgcg tttatcgtta caacaccaac
    10141 ggcggcatcg ttaccggtga cggtatgggt atggcgctaa gccacggcgt tccgctgcgt
    10201 gacatggaat tcgttcagta tcacccaacc ggtctgccag gttccggtat cctgatgacc
    10261 gaaggctgcc gcggtgaagg cggtattctg gtcaacaaaa atggctaccg ttatctgcaa
    10321 gattacggca tgggcccgga aactccgctg ggcgagccga aaaacaaata tatggaactg
    10381 ggtccacgcg acaaagtttc tcaggccttc tggcacgaat ggcgtaaagg caacaccatc
    10441 tccacgccgc gtggcgatgt ggtttatctc gacctgcgtc acctcggcga gaaaaaactg
    10501 catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt cgatccggtt
    10561 aaagaaccga ttccggtacg tccgaccgca cactacacca tgggcggtat cgaaaccgat
    10621 cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc ctctgttggt
    10681 ctgcacggtg caaaccgtct gggttctaac tccctggcgg aactggtggt cttcggccgt
    10741 ctggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa cgaagcggca
    10801 attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctggttaa ccaggatggc
    10861 ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg caatggaaga aggttgcggt
    10921 atctaccgta cgccggaact gatgcagaaa accatcgaca agctggcaga gctgcaggaa
    10981 cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt tcaacaccga cctgctctac
    11041 accattgaac tgggccacgg tctgaacgtt gctgaatgta tggcgcactc cgcaatggca
    11101 cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccga gcgtgacgac
    11161 gtcaacttcc tcaaacacac cctcgccttc cgcgatgctg atggcacgac tcgcctggag
    11221 tacagcgacg tgaagattac tacgctgccg ccagctaaac gcgtttacgg tggcgaagcg
    11281 gatgcagccg ataaggcgga agcagccaat aagaaggaga aggcgaatgg ctgagatgaa
    11341 aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac cgcatagcgc
    11401 attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg gctacatcaa
    11461 agacaacctg gcaccggacc tgagttaccg ctggtcctgc cgtatggcga tttgtggttc
    11521 ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta
    11581 caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacgcg atctggtggt
    11641 cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca tcggcaactc
    11701 ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcaa agtatcacca
    11761 gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa
    11821 cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg aagatagccg
    11881 cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggcg tatggagctg
    11941 tactttcgtg ggctattgct ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat
    12001 tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga aaccacgcta
    12061 aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg tccacctggt
    12121 ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg gttccggctg
    12181 tgtggttcag cattgaactg attttcgggc tgtttgccct gaaaaatggc ccggaagcct
    12241 gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt gatcattaac ctgatcactc
    12301 tggcggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa gcggccaata
    12361 tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc tgggcggtaa
    12421 ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga gcctgagatg
    12481 attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt cggggccggt
    12541 ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat tctgctgcca
    12601 ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt cgcgcagagc
    12661 ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg tggtttacac
    12721 cgtatgcacc acgcgatgca cgatctgaaa attcacgtgc ctgcgggcaa atgggttttc
    12781 tacggtctgg ctgctatcct gacagttgtc acgctgattg gtatcgttac aatctaacgt
    12841 atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa ccagacctta
    12901 tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttgctccaca tttgctgccc
    12961 ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag caacaaaaga
    13021 tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt acctggggct
    13081 atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag ttaggttcgg
    13141 tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt
    13201 taagcgatcc cacaacaaaa tactggcctg aacttaccgc taaacagtgg aatgggatca
    13261 cactattaca tctcgcaacc tacactgctg gcggcctgcc attgcaggtg ccggatgagg
    13321 tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca tgggcgccag
    13381 gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg gctgtgaagc
    13441 cgtctggttt gagttttgag caggcgatgc aaacacgtgt cttccagcca ctcaaactca
    13501 accatacgtg gattaatgta ccgcccgcag aagaaaagaa ttacgcctgg ggatatcgcg
    13561 aaggcaaggc agttcatgtt tcgccagggg cgttagatgc tgaaacttat ggtgtgaagt
    13621 cgaccattga agatatggcc tgctgggtac gaagcaatat gaatccccgt gatatcaacg
    13681 acaaaacact tcagcaaggg atacaactgg cacaatctcg ctactggcaa accggcgata
    13741 tgtatcaggg cctgggctgg gaaatgctgg actggccggt aaatcctgac agcatcatta
    13801 acggcagtgg caataaaatt gcactggcag cacaccccgt aaaagcgatt acgcccccaa
    13861 ctcctgcagt acgcgcatca tgggtacata aaacaggggc aaccggcgga tttggtagct
    13921 atgtcgcgtt tattccagaa aaagagctgg gtatagtgat gctggctaac aaaaactatc
    13981 ccaatccagc gagagtcgcc gccgcctggc agattctcaa cgctctacag taaaatttca
    14041 tcgggtccga attttcggac cttttctgcg cttttccttg ctgtcatcta cacttagaaa
    14101 aaaaccagta aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc gacagctgca
    14161 tttctggtcg ttgcctgcag ttctcctacg ccgccgcgtg gcgtaacagt agtgaataat
    14221 ttcgacgcca aacgttatct cggtacctgg tatgagattg cccgttttga tcaccgtttt
    14281 gaacgtggac tggaaaaagt caccgcaaca tacagcctgc gtgatgacgg cggcctgaat
    14341 gtcattaata aaggctataa ccctgacaga ggaatgtggc agcagagtga agggaaagcg
    14401 tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt cattctttgg tcctttctat
    14461 ggcggttata acgttattgc actcgatcgg gaataccgcc atgcgctggt ttgcggcccg
    14521 gaccgcgact acctgtggat actctcccgc acgccaacca tttctgacga agtgaaacag
    14581 gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca gtaagtttat ttgggtacag
    14641 cagcccggta gttagtgagt gctgagtttc agaccaataa tccccaatac gattaacgcc
    14701 agactcgcca ggcgcatcgg gttagcggac tcaccgagca gcacaatgcc agtgatagcc
    14761 gcgccgactg cgccaatacc cgtccacacg gcataagccg tccctactgg taacgatttc
    14821 atcgcccagg caagtagcgc catactgaca atcatcgccg tcacggtaat aacactcggc
    14881 gtcaaacgac taaagccgtg ggtatatttc aggccaacgg cccataccac ttccagcaga
    14941 ccagcaataa ctaagataat ccaggacata tcaggctccg gagaattggg gccgtccccg
    15001 gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga tattttgcca
    15061 ggtggcaggg gaatttcaac tattacctta gtgactatcg cagaaaaaac aaacggcacg
    15121 acatacctgt cgtaccgtta ttgcttattg ctattgctgc gctttcgttg ctgcgccaga
    15181 aatcgcgtta ccgccatcag aaatgtcttc accaacgcca cgcgtggtgt tgcaggcagt
    15241 taatactgtt gaaagcacca gaacagaaaa gatcgctgca attgtcttct tcaccataac
    15301 gtcttccttt taggtaatgt tatttatgtt tgcctatagc aaattaataa tagacaacat
    15361 cgccaaaaat gacggaaatc tgatgatttt tcggaagaga agaaaaatta gctggcagcg
    15421 cgagagatgg agttgccgag atgtttgatg tcttcgccga aaccgcgagc ggtgttacag
    15481 cccgtgagca gcgtgctggc aagcaaaaca agaacgataa ggcgtttcat catctctgac
    15541 ctgaataagt gatggtgcag cctgcaggcc gcaccacaac cgcattactt cacgcgagag
    15601 acgtattcac cagagcgggt atccactttg atgacttcgc cgatttgtac gaacagcgga
    15661 accttaacca cagcgccagt agacagggta gccggtttgc cgccagtacc tgcggtatca
    15721 cctttcaggc ccggatcggt atcaacgatt tccagttcaa cgaagttcgg cggagtaacg
    15781 gagatcggct gaccattcca cagagttacg atacactctg cctgatccag cagccattta
    15841 gcgttgtcgc caattgcttt tgcatcagca gacagctgct cgaaagtttc gttgttcatg
    15901 aagtgccaga actcaccgtc gttgtacagg taagtcaggt tcatatcgac aacatcagcg
    15961 ccttcagcgg aatcagtaga tttgaaggtt ttttctacgc gagtaccggt cagcagacga
    16021 cgcagtttaa cgcgagcaaa tgcctggcct ttacccggtt ttacgaattc actcgcttca
    16081 accgcgtaag gttcgccgtc taacatgatt ttaagaccag cacgaaaatc gttgctatag
    16141 tacgttgcca taaggccctc tgaaatttgt taattggtag ctaagccaca aaatggcgca
    16201 tattgtaacc ctaaataccc catccagaga agattggtta acgcaacttg ccgatgttgt
    16261 gaccgatcct gatgaacttc tgcgtctttt gaatatagac gcggacgaaa aactgttggc
    16321 cggacgcagc gccaaaaagc tgtttgccct gcgtgtgccc cgctcattta tcgatcgcat
    16381 ggagaaaggc aatccggacg atcctctttt gcgtcaggta cttacctcgc aagatgagtt
    16441 tgtcgtcgtg tccggattct ccaccgaccc gctggaagaa cagcacagcg tagtgcctgg
    16501 tttgttgcat aaataccaca accgggcgct tttgctggtc aaaggcggct gcgcggtaaa
    16561 ttgccgctat tgcttccgtc gccacttccc ctatgccgaa aatcagggca acaagcgtaa
    16621 ctggcagact gctcttgagt atgttgctgc gcatccggaa ctggacgaga tgattttctc
    16681 cggcggcgat ccgctgatgg cgaaagatca cgagctggac tggttgctca cacaactgga
    16741 agccatcccg catatcaaac gtctgcggat tcacagccgt ctgccgattg tgatcccggc
    16801 acgtatcacc gaggcgctgg ttgaacgctt tgcccgttct acgctgcaaa tcttgctggt
    16861 gaatcacatc aatcatgcca atgaggtaga tgaaacgttc cgtcaggcga tggctaaatt
    16921 gcgccgtgtc ggtgtcaccc tgcttaacca gagcgttctg ttacgtggtg tgaacgataa
    16981 cgcacaaacg ctggcaaacc tgagtaatgc gttgttcgat gccggcgtaa tgccgtatta
    17041 cctgcatgtg ctcgataaag tacagggcgc ggcgcatttt atggtgagtg atgacgaagc
    17101 acggcagatt atgcgtgagt tgctgacact ggtgtcgggt tatctggtgc cgaaactggc
    17161 gcgagaaatc ggcggcgaac ccagcaaaac gccgctggat ctccagctac gccagcagta
    17221 actgattaaa cagggaagtt aaacattagc ctgatgcgct acgcttatca ggcctacatt
    17281 gtgcattgaa attgaatgga tttgcaatga actgtaggcc ggataaggcg tttacgccgc
    17341 atccggcaag agcaacaaac gcaagtacag caacctgtca ttaaactggg aaatttaatc
    17401 tttgcccgtt gcataacctt taattattca tatacattgc tgttgttact ctttatatag
    17461 ccaacaccaa taattaacct ttcttttctg tttatataga gtgagtacgg ctatttatgt
    17521 tattttaaca actattacta ccaagcccat ttatctggct tataaattaa aattaattat
    17581 ttaattgaga aatggttagg gagaacctac atggccataa gcatcaaggg tgttaatacc
    17641 ggtgttattc gcaagagtaa taatttcatt gcactggcgc tgaaaattaa agaacctcgc
    17701 aataaagaat cgttgttctt tatgtctgtc atggaactgc gcgacctgct gattgcgctg
    17761 gaaagtcgta tgcatcaaaa gcataaactg gatgctgctg cacgccttca gtatgagcaa
    17821 gctcgcgata aagtcattaa gaaaatggca gaaaacatcc cagaaattct ggttgatgag
    17881 cttaaaaatg ccgatattaa tcgtcgggtt aacacgctgg aacttaccga taatcaaggc
    17941 gaaaatctta ccttcgtatt aacgctacat gacggtagca aatgtgagtt agtcgtcaat
    18001 gagttgcaga ttgaaatgct ggcgcgagca atcattcatg caattaataa tgcggaaatg
    18061 cgtgaactgg cattacgtat tacctcactg ttagatttcc tgccgttata tgacgttgac
    18121 tgccaggaaa atggcaatct ggaatatgac acctattccc aaccggaatg gaaacataac
    18181 ctgttcgacc attatctggc ggtgctttac cgctttaaag atgaaagtgg caaagaacaa
    18241 tttagcggcg cagtggtgaa aacgcgtgaa gcaacgccgg gtaaagaaat agaggcgatc
    18301 actcgccgaa tgcttgattt cagccaacga ctgaaaaaac tcgcgggtgt gccatgtcag
    18361 gtctatgttc gcaccgtagc ggcgaataac gcccaaccgc taacccagga tcaatgctta
    18421 cgcgcgctgc atcacttacg tgttcaatcc accagcaaaa ctgcaccaca ggcgaaataa
    18481 tctcatcttt gctaatgcga ggctggagcc atccagcctg aaatgactga taacgtgatc
    18541 gttggtcatg ccggatgcgg cgtaaacgcc ttatccggcc tacaa
//
LOCUS       DAZGSP010000415         6109 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.guided.191, whole genome shotgun sequence.
ACCESSION   DAZGSP010000415 DAZGSP010000000
VERSION     DAZGSP010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.guided.191"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            49..1188
                     /locus_tag="ACHK74_005511"
     CDS             49..1188
                     /locus_tag="ACHK74_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFP0224117.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALIGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            1185..1799
                     /gene="prfH"
                     /locus_tag="ACHK74_005512"
     CDS             1185..1799
                     /gene="prfH"
                     /locus_tag="ACHK74_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFP0224118.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1856..3313)
                     /gene="pepD"
                     /locus_tag="ACHK74_005513"
     CDS             complement(1856..3313)
                     /gene="pepD"
                     /locus_tag="ACHK74_005513"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP0224119.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3574..4032
                     /gene="gpt"
                     /locus_tag="ACHK74_005514"
     CDS             3574..4032
                     /gene="gpt"
                     /locus_tag="ACHK74_005514"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP0224120.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4124..5368
                     /gene="frsA"
                     /locus_tag="ACHK74_005515"
     CDS             4124..5368
                     /gene="frsA"
                     /locus_tag="ACHK74_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP0224121.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            5426..5827
                     /gene="crl"
                     /locus_tag="ACHK74_005516"
     CDS             5426..5827
                     /gene="crl"
                     /locus_tag="ACHK74_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP0224122.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5866..>6109)
                     /locus_tag="ACHK74_005517"
     CDS             complement(5866..>6109)
                     /locus_tag="ACHK74_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFP0224123.1"
                     /translation="AQYQFDFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFN
                     KNMSAFVDYKINQLDSDNKLNINNDDIVAVGMTYQF"
BASE COUNT         1492 a         1483 c         1617 g         1517 t
ORIGIN      
        1 gcaacacatg caacttgaag tatgacgagt ctatttacgg aatggtatat gggcaattat
       61 attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg gatcgagagt
      121 ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg cgtagtgggg
      181 atgccagatt tacatcccgg acgcggctat ccgattggcg cagcgttctt ctctgttggt
      241 cgtttttatc cggcactgat cggcaatgat atcggctgcg gtatggcgct atggcaaaca
      301 gatattctca ctcgcaaata caacgccgat aagtttgaaa agcgattatc agatctggat
      361 gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc acagcatccg
      421 tggcgcagct cgcttggctc tatcggtggc ggcaatcatt tcgctgaact gcaacaagtt
      481 gatcaaatca tcaacgctga actgtttgca ctggcaggtc tggatgcaca acatctgcaa
      541 ctgttggttc atagcggctc gcggggggtg gggcagtcta ttttacagcg gcatattgcc
      601 tcgttctcgc atcatggttt gcctcaaggc agtgacgacg cgctacggta tattgcggaa
      661 catgacgatg cgctggcgtt tgcgtgtatt aatcgccaga tgatcgcttt gcgcataatg
      721 caacaagtta aggccacggg aagtccggtt ctggatgtgg cgcataactt tgttagcgcg
      781 tgtcaaatcg gcgatcaaca gggctggttg catcgtaaag gtgccacgcc ggatgacaac
      841 ggtctggtga ttattcccgg ttcacgtggt gattactcct ggattgtaca gcctgttgcg
      901 aacgaaaaaa cgctccactc gctggcgcat ggggcagggc gtaaatgggg gcgcaccgag
      961 tgtaaagggc gtctggcagc gaaatacacg gcgacgcaac tctcgcgtac tgaacttggc
     1021 agtcgggtaa tttgtcgcga taaacaactc atctttgaag aagcgccaca agcctataaa
     1081 tcggctgaaa gcgtggtgca atgtctggtg caggccggtt taattattcc tgtcgcgcgg
     1141 ctgcgtccgg tgctaacgct caaaaacagt ggagggaaaa aaggatgatc ttgctacaac
     1201 tctcctcagc gcaggggccg gaagaatgtt gtctcgcagt gaaaaaggcg ctggacaggc
     1261 taattaaaga agctgcccga caagacgtcg cggtaacggt gttggaaaca gaaacgggcc
     1321 gctactctga cacgctgcgt tcggcgctga tttctctgga tggcgataac gcatgggcat
     1381 taagcgaaag ctggtgtggc actattcagt ggatttgtcc gagtccgtat cggcctcatc
     1441 atgggcgcaa aaactggttt ctgggcattg ggcgttttac cgctgatgag caggaacaat
     1501 cggatgcaat ccgttatgag acgttgcgtt cgtcggggcc gggcggtcaa catgtcaata
     1561 aaaccgactc ggcggtacgc gccacgcatt tggcatccgg tattagcgtg aaggttcagt
     1621 cagagcgtag tcagcatgct aacaagcggc tggcacgatt gctgattgcc tggaagctgg
     1681 agcagcagca acaggaaaat agcgcggtgc tgaaatcgca gcggcgaatg ttccatcacc
     1741 agattgaacg tggcaacccg agacggacat ttacagggat ggcttttatc gaaggataat
     1801 gaaggatgaa actgccggaa ggcgattaaa cgccatccgg cagcaaatca aataattact
     1861 tcgccggaat ttctttcagc agttcagtca gcagcgtcca gtaatgacct acgctttcga
     1921 tgtgaacttg ctcatccgga gagtgtggac cggtgatagt tggcccgata gaaaccatgt
     1981 ccatttccgg atacggtttt ttgaacagac cacattccag gcccgcgtgg ataatctgga
     2041 tgttcggtgt tttgttgaac agacgctgat aggtttcacg taccagatgc atcaccggag
     2101 aattagcgtc cggctgccag ccaggatatg cgcctttcgc ttcggttttc gcgccagcca
     2161 gtttacccag cgaatccagc atgctcacga cgtagtcttt accgctgtcg atcagtgaac
     2221 ggatcagaca gtgaatttct acgttattat cagtcatggt caccacaccg acgttcaggg
     2281 aggtttcgac cacacctttt gccacgtctg agttgcggat cacaccgttc ggggtggcgt
     2341 tcagcagacg aataaaggta tcgcgagatt tcgcaatcag ggcagcttta tcgttcgcta
     2401 cagagtccag caacaaggcc agatttttct ctttctctgc cagctcgttt ttcaggatct
     2461 cctgataggt attcaccaga gatttcagga cgtcgacttt atcagctgcg acagcaatgg
     2521 tcgcaaaggc ttcacgcggg atggcgttac gcagtgtgcc gccgttgaaa tcgataaggc
     2581 gcagatccag ttcttctgca tgacccgcca ggaagcgcac cagcagtttg ttggcattac
     2641 ccaggccaac gtggatttcc ccgccggagt gacccccttt cagacctttt aaggttaact
     2701 tgaaggtttc aaaaccagct ggaaccgctt cacgatctaa atgcaggttg gaggtgaagt
     2761 cgataccccc cgcacaaccc atgtagattt caccttcttc ttcggagtcg gtgttaatca
     2821 gaatatcagc ctgcaaccag ttgctctgta agccgaacgc accgtccata ccggcttctt
     2881 cggtcatggt cagcagcact tccagcgggc cgtgaaccac gttttcgtca gccagaactg
     2941 ccagtgcaga ggccatacca atgccgttat ccgcacccag cgtggtgccg cgcgctttaa
     3001 cccattcgcc atcaatataa ggctggatag gatctttcgt gaagtcatgc acggtgtcgt
     3061 tatttttctg cggcaccata tcgaggtggg cctgtaagac gaccggttta cgattttcca
     3121 tacctgcggt agcaggttta cgaatcagga tattacctac ctgatcgcgt tcgacatgga
     3181 aacctttctc ttttgcccaa ccaacaatgt attcagcgag ttgctcttca tgataggacg
     3241 ggtgaggaat agaacagatt ttggcaaaaa tatcccacag cggctgtgga gataattgag
     3301 acagttcaga cacgttaagt ctccttgtcg atcacccgca aaacagtatt gcaggtcaca
     3361 gggttagcag aaaatgttgt caacacaaga caggcttgcg agatatgttt gagaatacca
     3421 ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg
     3481 gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt
     3541 taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg
     3601 gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg
     3661 aaaggcatta ttgccgtaag ccgtggcggt ctggtaccgg gtgcgttact ggcgcgtgaa
     3721 ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc
     3781 gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac
     3841 ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt
     3901 gtcaccatct ttgcaaaacc ggctggtcgt ccgctggttg atgattatgt tgttgatatc
     3961 ccgcaagata cctggatcga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc
     4021 tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg
     4081 cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct
     4141 gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg
     4201 ctttaatcac ggcgcacaac cgcctgtgca gtcggctctt gatggtaaaa ccatccctca
     4261 ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggtattgacc cacgtgaaat
     4321 cctagacgtc caggcacgta ttgtgatgag cgatgctgaa cgtaccgacg atgatttata
     4381 cgatactgtg attggctacc gtggtggcaa ctggatttat gagtgggcca cccaggcgat
     4441 ggtgtggcaa caaaaagcct gtgcggaaga agatccgcaa ctcagtggtc gacactggct
     4501 gcatgcggct acgttgtaca acattgccgc ctatcctcat ctgaaaggag atgacctggc
     4561 agagcaagcg caggctttat caaaccgcgc ctatgaagag gccgcccagc gtctgccggg
     4621 cacgatgcgg cagatggagt ttactgtacc cggcggtgcg cccatcaccg gctttttgca
     4681 tatgccgaaa ggcgatggcc cgttcccgac agtattaatg tgtggtggtc tggatgcgat
     4741 gcagacggac tattacagcc tgtatgaacg ttattttgcg ccgcgcggca ttgcgatgct
     4801 gactattgat atgccgtcgg tgggcttttc ttcaaaatgg aagctcaccc aggactccag
     4861 cctgttgcat cagcacgtct taaaggcgct gcctaacgta ccgtgggtgg atcacactcg
     4921 cgtcgcggcc tttggtttcc gtttcggcgc taacgttgcc gtgcgtctgg cataccttga
     4981 atcgccgcgt ctgaaagcgg ttgcctgtct tggtccggta gttcataccc tgttgagtga
     5041 ttttaagtgc cagcaacagg tgccggaaat gtatcttgac gttctggcga gtcgtttggg
     5101 gatgcatgat gcttccgacg aagcgttgcg cgtggagttg aatcgctatt cattaaaagt
     5161 gcaaggattg ctgggacgtc gctgcccaac gccaatgtta tcaggctact ggaagaacga
     5221 tccgttcagc ccggaagagg actcacgctt aatcacctca tcatctgctg acggtaaatt
     5281 attagagatc ccatttaacc cggtgtatcg gaattttgac aaaggtcttc aggaaatcac
     5341 cgactggatc gaaaaacgtt tgtgttaaaa atttgctaaa ttttaccaat ttggtaaaac
     5401 agttgcatca caacaggaga ttgcaatgac gttaccgagt ggacacccga agagcagatt
     5461 gatcaaaaaa tttaccgcac taggcccgta tattcgtgaa ggtaagtgcg aagataatcg
     5521 attctttttc gattgtctgg ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga
     5581 gttctggggc tggtggatgg agctggaagc gcaggaatcc cgttttacct acagctacca
     5641 gtttggtctg ttcgataaag caggcgactg gaagagtgtt ccggtaaaag atactgaagt
     5701 ggttgaacga ctggagcaca ccctgcgtga gtttcacgag aagctgcgtg aactgctgac
     5761 gacgctgaat ctgaagctgg aaccggcgga tgattttcgt gacgagccgg tgaagttaac
     5821 ggcgtgagtg aaatgtgccg gatgcatcac atccggcaat atctattaaa actgataggt
     5881 catgccaacc gcgacaatat catcattatt aatattcaat ttgttatcgc tatccagttg
     5941 gttgatttta taatcaacaa acgctgacat atttttgttg aaataatatg tagccccgac
     6001 atcgatataa ttgaccagat cttcatcacc gataccttca atatctttcc ctttcgataa
     6061 gacataaccc agcgatggac gcagaccaaa gtcaaactgg tattgagcg
//
LOCUS       DAZGSP010000416         1927 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.guided.270, whole genome shotgun sequence.
ACCESSION   DAZGSP010000416 DAZGSP010000000
VERSION     DAZGSP010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.guided.270"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            130..1089
                     /gene="stxA2b"
                     /locus_tag="ACHK74_005518"
     CDS             130..1089
                     /gene="stxA2b"
                     /locus_tag="ACHK74_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFP0224124.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRSVNE
                     EIQPECQITGDRPVIRLNNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1102..1365
                     /gene="stxB2b"
                     /locus_tag="ACHK74_005519"
     CDS             1102..1365
                     /gene="stxB2b"
                     /locus_tag="ACHK74_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFP0224125.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          517 a          411 c          463 g          536 t
ORIGIN      
        1 gcaatcggtc actggttcga atccagtaca acgcgccata attatttacc aggctcgctt
       61 ttgcgggcct tttttatatc tgcgccgggt ctggtatcgt attacttcag ccaaaaggaa
      121 tacctgtata tgaagtgtat attgttaaaa tgggtactgt gcctgttact gggcttttct
      181 tcggtatcct attcccggga atttacgata gacttttcga ctcaacaaag ttatgtctct
      241 tcgttaaata gtatacggac agaaatatcg accccacttg aacatatatc tcaggggacc
      301 acatcggtat ctgttattaa ccacacccca ccgggcagtt attttgctgt ggatatacga
      361 gggcttgatg tctatcaggc gcgttttgac catcttcgtc tgattattga gcaaaataat
      421 ttatatgtgg ccggattcgt taatacggca acaaatactt tctacagatt ttcagatttt
      481 gcacatatat cagtgcccgg tgtgacaact gtttccatga caacggacag cagttatacc
      541 actctgcaac gtgtcgcagc gctggaacgt tccggaatgc aaatcagtcg tcactcactg
      601 gtttcatcat atctggcgtt aatggagttt agtggaaatg ccatgaccag agatgcatcc
      661 agagcagttc tgcgttttgt cactgtcaca gcagaagcct tacggttcag gcaaatacag
      721 agagaatttc gtctggcact gtctgaaact gctcctgttt atacgatgac accggaagaa
      781 gtggacctca cactgaactg ggggagaatc agcaatgtgc ttccggagtt tcggggagag
      841 ggtggtgtca gagtggggcg aatatccttt aataatatat cagcgatact gggcacagtg
      901 gcggttatac tgaattgcca tcatcagggg gcacgttccg ttcgctccgt gaatgaagag
      961 atacaaccag aatgtcagat aactggcgac aggccagtta taaggctaaa caatacttta
     1021 tgggaaagta ataccgcagc tgcttttctg aatcgcaggg ctcactcttt aaatacatcc
     1081 ggagaataac gggagttaaa tatgaagaag atatttgtag cggctttatt tgcttttgtt
     1141 tctgttaatg caatggcagc tgattgtgca aaaggtaaaa ttgagttctc taagtataat
     1201 gagaatgata cattcacagt aaaagtggcc gggaaagagt actggactaa ccgctggaat
     1261 ctgcaaccgc tactgcaaag cgcacagtta acaggaatga cggtaacaat caaatcaaat
     1321 acctgtgcgt caggttcagg atttgctgaa gtgcagttta attaatatca gaagcattgc
     1381 tggtttcgta gtgtgcagca atgtagttac agtgtaatca atgtcacaat tcagtcagtt
     1441 gacggttgcc tgtctggctg agcatttgtt aaaaaaatcc tgcatgatga atccccctgt
     1501 gcggaggggc ataatgacag atgtttggtt gcgtattgta taggcaagtt gcggattctg
     1561 tctggtcatt gcagaattca ccgggaggca cccggcatca tgctgtatac agagattagg
     1621 catatatcca ggctcctcat cgcaggagcc tttttacatg caaaaaaagc ccgcgccggg
     1681 agacgcgggc ggcaaggaat aaacaacaaa acgtgaagta atatttcagc tggcgaataa
     1741 tacccgacag taatcactct gcgcaactgc gcggcctttt tcgtattgcg ggctgtagtc
     1801 ttccttctgt cattgtcctt taacttccgg acctcagccc gctctttatc tgattcagta
     1861 cactatcccg gccgggagga ttcatgacat ttaaacacta tgacgtggtc agggcggcgt
     1921 cgccgtc
//
LOCUS       DAZGSP010000417         1927 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1909H49474
            SAMN43267026-rid23075213.guided.271, whole genome shotgun sequence.
ACCESSION   DAZGSP010000417 DAZGSP010000000
VERSION     DAZGSP010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267026
            Sequence Read Archive: SRR30307435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350753.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 91
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 87 of 192
            Pseudo Genes (incomplete)         :: 100 of 192
            Pseudo Genes (internal stop)      :: 45 of 192
            Pseudo Genes (multiple problems)  :: 33 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267026-rid23075213.guided.271"
                     /serotype="O128:H 2"
                     /isolate="1909H49474"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-08"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            130..1089
                     /gene="stxA2b"
                     /locus_tag="ACHK74_005520"
     CDS             130..1089
                     /gene="stxA2b"
                     /locus_tag="ACHK74_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFP0224126.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRSVNE
                     EIQPECQITGDRPVIRLNNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1102..1365
                     /gene="stxB2b"
                     /locus_tag="ACHK74_005521"
     CDS             1102..1365
                     /gene="stxB2b"
                     /locus_tag="ACHK74_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFP0224127.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          516 a          411 c          464 g          536 t
ORIGIN      
        1 gcaatcggtc actggttcga atccagtaca acgcgccata attatttacc aggctcgctt
       61 ttgcgggcct tttttatatc tgcgccgggt ctggtatcgt attacttcag ccaaaaggaa
      121 tacctgtata tgaagtgtat attgttaaaa tgggtactgt gcctgttact gggcttttct
      181 tcggtatcct attcccggga atttacgata gacttttcga ctcaacaaag ttatgtctct
      241 tcgttaaata gtatacggac agaaatatcg accccacttg aacatatatc tcaggggacc
      301 acatcggtat ctgttattaa ccacacccca ccgggcagtt attttgctgt ggatatacga
      361 gggcttgatg tctatcaggc gcgttttgac catcttcgtc tgattattga gcaaaataat
      421 ttatatgtgg ccggattcgt taatacggca acaaatactt tctacagatt ttcagatttt
      481 gcacatatat cagtgcccgg tgtgacaact gtttccatga caacggacag cagttatacc
      541 actctgcaac gtgtcgcagc gctggaacgt tccggaatgc aaatcagtcg tcactcactg
      601 gtttcatcat atctggcgtt aatggagttt agtggaaatg ccatgaccag agatgcatcc
      661 agagcagttc tgcgttttgt cactgtcaca gcagaagcct tacggttcag gcaaatacag
      721 agagaatttc gtctggcact gtctgaaact gctcctgttt atacgatgac accggaagaa
      781 gtggacctca cactgaactg ggggagaatc agcaatgtgc ttccggagtt tcggggagag
      841 ggtggtgtca gagtggggcg aatatccttt aataatatat cagcgatact gggcacagtg
      901 gcggttatac tgaattgcca tcatcagggg gcacgttccg ttcgctccgt gaatgaagag
      961 atacaaccag aatgtcagat aactggcgac aggccagtta taaggctgaa caatacttta
     1021 tgggaaagta ataccgcagc tgcttttctg aatcgcaggg ctcactcttt aaatacatcc
     1081 ggagaataac gggagttaaa tatgaagaag atatttgtag cggctttatt tgcttttgtt
     1141 tctgttaatg caatggcagc tgattgtgca aaaggtaaaa ttgagttctc taagtataat
     1201 gagaatgata cattcacagt aaaagtggcc gggaaagagt actggactaa ccgctggaat
     1261 ctgcaaccgc tactgcaaag cgcacagtta acaggaatga cggtaacaat caaatcaaat
     1321 acctgtgcgt caggttcagg atttgctgaa gtgcagttta attaatatca gaagcattgc
     1381 tggtttcgta gtgtgcagca atgtagttac agtgtaatca atgtcacaat tcagtcagtt
     1441 gacggttgcc tgtctggctg agcatttgtt aaaaaaatcc tgcatgatga atccccctgt
     1501 gcggaggggc ataatgacag atgtttggtt gcgtattgta taggcaagtt gcggattctg
     1561 tctggtcatt gcagaattca ccgggaggca cccggcatca tgctgtatac agagattagg
     1621 catatatcca ggctcctcat cgcaggagcc tttttacatg caaaaaaagc ccgcgccggg
     1681 agacgcgggc ggcaaggaat aaacaacaaa acgtgaagta atatttcagc tggcgaataa
     1741 tacccgacag taatcactct gcgcaactgc gcggcctttt tcgtattgcg ggctgtagtc
     1801 ttccttctgt cattgtcctt taacttccgg acctcagccc gctctttatc tgattcagta
     1861 cactatcccg gccgggagga ttcatgacat ttaaacacta tgacgtggtc agggcggcgt
     1921 cgccgtc
//
